LOCUS       DATMSX010000001        10026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000001 DATMSX010000000
VERSION     DATMSX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VN041_00005"
     CDS             <1..1108
                     /locus_tag="VN041_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWU27460.1"
                     /translation="YGHADYTHTEFEAVGEPGTIFNSDGWEGRADLIQRERNGWNGAV
                     GVQALSRNFEAIGEEAFVPSVKIDELGLYTVQRLDLGNHGFEGGLRYDRRELSGTPIG
                     GASEVTREFDNWSASAAAFIRPTAGLFLGLSLAHNERAPSEVELFADGVHIATAAYET
                     GDLTLNSEKVTTLEGTAHYDRGRFTGDLHVYHSWYNGFIDERPTGDQFYFEEEDEFFP
                     IYRFVQTDAKFYGFELEGAYALWQDGDRKLSLEGAADYVHAQTDFGPAARIPPYSVTG
                     RLAWTSTKFDASAEVRHVGEQDRVANEFELPTDDYTLVNASVAVRPFAQQNVTLFAEA
                     RNLTDEEAREHVSFLKDIAPLPGRNLRVGVAYRF"
     gene            1199..1360
                     /locus_tag="VN041_00010"
     CDS             1199..1360
                     /locus_tag="VN041_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27461.1"
                     /translation="MTDKEPRQVPIGYEDRPDKPTRGAFRITALAIAAAAVVFLTLIA
                     VVVVRSGGI"
     gene            complement(1643..3274)
                     /locus_tag="VN041_00015"
     CDS             complement(1643..3274)
                     /locus_tag="VN041_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU27462.1"
                     /translation="MSIREIATDRRTFLKYVGFGALSVAAVGSLAGCGTKTELKASGA
                     GSVKMPTFLTSEGLTPDLKGTAEGVPSAFFNYPKNPVRSVKTPPLKGETFSAITNIFG
                     PPPNGRDKNPAWQEIEKRLGGRLDITAVSADDFATKINTTLAGNDIPDMILDDGASIP
                     DIIGFLQAKCQDLTKFLSGDAVKDYPNLAAIPSLYWEQTVRDGKIYALPIPRGGTAGL
                     GFYNAKRYADVGVTDTSKIKDADEYLSIMKELTDAKANRWAFGSTSYGLATFFQLFGV
                     PNQWREEKGKMTFQIETDEYLEAISFVAKAAKAGYIMPGSDGWTKSQMVNAFTNGQVA
                     QIYDGLPGYFKADGYAVTVPDSAPFLPFAAGGGKPQANLDNIVFATTMIKKNDDASVK
                     KLLGIANLLAAPFGTEEYLLINYGVEGADYTRDGNGNPVPTANALADTTVPWRYLTAP
                     APALYLPGHDDVIRKTHEAYAAMIPLGVANPAATLFSPTDASKGNTIRQPVSDATQDY
                     ISGRKTLDDVKAAIKAWKSAGGDAIRKEYEKALKA"
     gene            complement(3332..4267)
                     /locus_tag="VN041_00020"
     CDS             complement(3332..4267)
                     /locus_tag="VN041_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU27463.1"
                     /translation="MSTESTTVVRLGRQRARDYRARTRPVWMEKPSFAMQGLKGLVLL
                     LVCVAVIYPFLVVIGTSLSTQQEIAANNGFVLIPMHPTLEAYRTILSGGIVTEALFRS
                     IGITLVGTALSLTLTVMMAYGLSRRIMGGNFFLMTALLTMLFSPGIIPTFLVVKELGL
                     LGTFAALILPTALSAFNLIIIRSFFMNVPSELVEAARLDGAGDIRILWNVMLPLSKAV
                     IAVIGLFYAVGYWNMFFNAMLYVDSDKWPLAMVLRQYVLLGSSIDANTGAEIAAPSQA
                     IQMAVVVISLVPILCVYPFLQKYFTKGVLTGAVKG"
     gene            complement(4257..5273)
                     /locus_tag="VN041_00025"
     CDS             complement(4257..5273)
                     /locus_tag="VN041_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU27464.1"
                     /translation="MVSTPVTSRPKAAADEPARPVTGHARRLDRKGRTRLSFAARFRR
                     DRVMLLLVMPGFLYFVVFHWLPIPGNIIAFEDYQPYLGFVDSVWVGLDNFAVAFSDPA
                     FWYALRNTLVIMVMQLVLFFPVPIALALLLNSIMSPKLKKFVQSVIYLPHFLGWVIIV
                     SLFTQILGPTGLIPNFLDSLGMGRISPMTDPATFPLLVTVQTIWKDAGWGTIMFLAAL
                     ASIDDSLYEAAAIDGAGPWRRTWAVTIPGIMPIVILLLILNLGTALSVGFEQIILQRN
                     NVGAQAGEVLDTYVYFHGIVDGQWGTSAAVGLVKGIVGLFLVLGANKVAHMFGQDGVY
                     SREH"
     gene            5479..6486
                     /locus_tag="VN041_00030"
     CDS             5479..6486
                     /locus_tag="VN041_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWU27465.1"
                     /translation="MSWHPRVAFAMNRDDHLAAFPASLLERLGSIAEVITPLPMEEFD
                     SAASRELLAETEVLITGWGAPRFGLATLAQAPHLRAIVHAAGTVRTFLDPAVYDRGIR
                     VSTCASANAIPVAEFTLASIVFGLKRASRFAERLRRTKAVRDTAGFPPIGTNGVTVGL
                     IGASRVGRETLRMLRTLDVRILLHDPYLSPASAAELGAELVGLDELCRRSDAVSVHAP
                     LTAQTVGMIGTHELSLMRDGTVLVNTARGAIIDTAALTTELVAGRLDAYLDVVHPEPL
                     PADSPLYGLPNVVITPHIAGALGNEVVRLGELAVDEVARFAASDTFDHEVRSADLAIT
                     A"
     gene            6508..7590
                     /locus_tag="VN041_00035"
     CDS             6508..7590
                     /locus_tag="VN041_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU27466.1"
                     /translation="MSSTSEQTRVRGLRRPATVSDVAAAAQVSTATVSRVLNGNYPVA
                     AATRERVMRAVSKLGYAANANARALTQSDTKTIGIIVPELVDPFFGYMVRGLERATQE
                     SGRLAIIATTSFRPGAEIALIDRMRERRVDAVIVVSGGSSDPAYRRQLAQRADALAAM
                     GSRLVLCAHPTLDLPSAARSVAYDTEGGAFSVTEHLIANGHRRIAMVGGESHLPTIGL
                     RRDGYLRALASRGIDIDEDLIRIEGFGRDVGYASTRALMEEHPDVTAIFAVNDTIASG
                     VYDALGDAGLRIPTDVSVVAYDDTPLAANLSPKLTTVHVPLEQLGQEALRAAIDEPDA
                     FSRTPEQGLTLGTYLVHRDSVAMPPR"
     gene            complement(7718..8506)
                     /locus_tag="VN041_00040"
     CDS             complement(7718..8506)
                     /locus_tag="VN041_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU27467.1"
                     /translation="MDWSIQEIARLAGTTSRTLRHYDDIGLLAPSRIAANGYRHYDEA
                     ALVRLQRILLLRELGLGLPQIAEVLGASTGSATQMAEASALQTHLELLRQEQQRIGRQ
                     IASVESTITALTEGKTLMAENMFDGFDHTQYKEEVEERWGRQAYADGDRWWRGLSDDD
                     RAAWKQRVADLNRDWIAAAESGIAPDSDEAQAIARRHVEWLTGVPGTPASAPGGDVKG
                     YVMGLGEMYVADPRFGANYATSDGGSAGAEFVRDALRIYAEANL"
     gene            8681..9520
                     /locus_tag="VN041_00045"
     CDS             8681..9520
                     /locus_tag="VN041_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWU27468.1"
                     /translation="MTEKWLIAPGEERVIDIASASRLKVGLVGGQVDVVAHDEPGIRI
                     EVHGVTIKDLRVESDGTQIEIDHPQIGWDNFLEVFRNFGAGGPKAEISVAVPRAIALT
                     LGVVSAGALVSGIRNDVKLNTVSGDIIADDVTGDLSVNSVSGDVQLRGLTGSVVANSV
                     SGDVAVTGSLRKAVIDTVSGDVLIDAIGDVNTMNVNTVSGGVTVRLDETLPANYALRS
                     MSGKLMVDGIQRSSGGPSNYTGQMGELAGSFVDVRANTVSGGITVLRQARPTVADDPE
                     WEE"
     gene            9520..>10026
                     /locus_tag="VN041_00050"
     CDS             9520..>10026
                     /locus_tag="VN041_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWU27469.1"
                     /translation="MAPAVFSHGDLRLYLLSLLNEAPQHGYGIIQALTDRTGGTYTPS
                     AGTIYPRLAKLEEEGLVTKTVEGRTTIYAITDAGRAELESRSSDLEGIEEGLSDSVRL
                     IASEVRQSVKEAMRSLRADLATAAQEERRAAKKQPRAEGLGDERVTSRENLQRADAAI
                     NAFRAQVRT"
BASE COUNT         1640 a         3399 c         3353 g         1634 t
ORIGIN      
        1 ctacggccat gccgactaca cccacaccga gttcgaggcc gtgggcgagc cgggcaccat
       61 cttcaactcc gacggctggg aaggtcgtgc ggacctgatc cagcgcgaac gcaacggctg
      121 gaacggcgcc gtcggggttc aggccctgtc gcgtaacttc gaggccatcg gcgaggaggc
      181 cttcgtgccc agcgtcaaga tcgacgaact gggcctctac acggtgcagc gcctggacct
      241 cggcaaccat ggcttcgagg gcggcctgcg ctatgaccgg cgtgaactca gcggcacgcc
      301 catcggcggc gcgagcgaag tcacgcgcga attcgacaac tggtcggcgt ccgccgccgc
      361 cttcatccgc ccgaccgccg gcctgttcct gggcctaagc ctggcccaca acgagcgcgc
      421 gcccagcgag gtcgaactgt tcgccgatgg cgttcacatc gccaccgccg cctacgagac
      481 cggcgacctg acgctgaact ccgagaaggt gacgacgctg gaaggcaccg cccactatga
      541 tcgcggccgg ttcaccggcg atctgcacgt ctatcactcc tggtataacg gcttcatcga
      601 cgaacggccg acgggcgacc agttctattt cgaggaagag gacgagttct tccccatcta
      661 tcggttcgtc cagaccgacg cgaagttcta tggcttcgag ctggaaggcg cctatgcgct
      721 ttggcaggac ggcgatcgca aactgtcgct ggaaggcgcg gccgactatg tccacgccca
      781 gacggacttc ggccctgccg cgcggatccc gccctactcc gtcaccggac gtctggcctg
      841 gacatcgacc aagttcgacg ccagcgccga ggttcgccac gtcggagaac aggaccgggt
      901 cgctaacgaa ttcgaactgc cgaccgacga ctacaccttg gtcaacgcct cggtcgcggt
      961 ccgtcccttc gcccagcaga acgtcaccct gttcgccgag gcgcggaacc tgacggacga
     1021 ggaagcccgc gaacacgtgt ccttcctcaa ggacatcgcc cctctgccgg ggcgcaacct
     1081 tcgggtcggc gtcgcctacc gcttctgacg aacatcgaga gccttcggcg cagccttggt
     1141 tgcgccgaag gctcacacgc gtcggcgaac gcgaaccgcc tccgtgcgtt gatcgcccat
     1201 gaccgacaaa gaaccccgcc aagtcccgat tggctacgaa gaccggcccg acaagccgac
     1261 acgcggcgcc tttcgcatca cggctctcgc catagcggcc gcagccgtcg tcttcctgac
     1321 gctgatcgca gtcgtcgtcg tgcgaagcgg cgggatctag gggcccaagg ccttccgacc
     1381 tgtatcgggg cgtggcttgc tgcacgtcct ttccaagcgc ttgaacttta taacagccgg
     1441 cgaagtggcc agcccgcgca aacccgcggc caccggccgt gaggctctac tacggaccgc
     1501 gacagatgat tgacttgaac accgccactt cggccgaact taatggcgtt gaacttttgc
     1561 gtggccatgg gtttgagatt gccggcggtc tccgtccaga gcagacggtc ggacgcgccc
     1621 cggccgggat cgtgctcggc ggtcacgcct tcagcgcctt ctcgtactcc ttgcggatcg
     1681 cgtcgccgcc ggcgctcttc cacgccttga tcgcggcctt gacgtcatcg agggtcttgc
     1741 ggccggagat gtagtcctgc gtcgcgtcgc tgacgggctg cctgatcgtg ttgcccttgg
     1801 acgcgtcggt cgggctgaac agcgtggcgg ccgggttcgc gaccccgagc gggatcatcg
     1861 cggcgtacgc ctcgtgcgtc ttcctgatca cgtcgtcgtg gcccggcagg tacagcgcgg
     1921 gcgccggggc ggtgaggtac ctccacggca cggtcgtgtc ggcgagcgcg ttcgcggtgg
     1981 ggaccgggtt gccgttgccg tcacgcgtgt agtcggcgcc ctccaccccg tagttgatca
     2041 ggaggtactc ctccgtgccg aacggggccg ccagcaggtt ggcgatgccg aggagcttct
     2101 tgaccgacgc gtcgtcgttc ttcttgatca tggtggtcgc gaagacgatg ttgtcgaggt
     2161 tggcctgcgg cttgccgccg cctgcggcga acggcaggaa gggcgcggag tccggcacgg
     2221 tcacagcgta gccgtcggcc ttgaagtacc cggggagccc gtcgtagatc tgcgcgacct
     2281 gtccgttggt gaaggcgttc accatctgcg acttggtcca tccgtcgctg ccgggcatga
     2341 tgtagccggc cttcgctgcc ttcgccacga aggagatcgc ctcgagatac tcgtcggtct
     2401 cgatctggaa ggtcatcttc cccttctcct cgcgccactg gttcgggaca ccgaacagct
     2461 ggaagaaggt ggccaggccg taggacgtcg agccgaacgc ccagcggttc gccttggcgt
     2521 cggtgagctc cttcatgatc gagaggtact cgtcggcgtc cttgatcttg ctggtgtcgg
     2581 tcaccccgac gtccgcgtag cgcttggcgt tgtagaagcc gaggccggcg gtgccgccgc
     2641 gcgggatggg cagcgcgtag atcttgccgt cgcgcaccgt ctgctcccag tacagcgacg
     2701 ggatcgcggc gaggttcggg tagtccttga cggcgtcgcc ggagaggaac ttcgtcaggt
     2761 cctggcactt ggcctgcagg aacccgatga tgtcggggat ggatgcgccg tcgtccagga
     2821 tcatgtccgg gatgtcgttg ccggcgagcg tggtgttgat cttggtcgcg aagtcgtcgg
     2881 cggagacggc ggtgatgtcg agcctgccgc cgaggcgctt ctcgatctcc tgccaagccg
     2941 ggttcttgtc acggccgttc ggcggcggac cgaagatgtt cgtgatggcc gagaacgtct
     3001 cgcccttcag aggcggggtc ttcacgctgc gcaccgggtt cttcgggtag ttgaagaacg
     3061 cggacggcac gccttcggct gtgcccttca ggtccggggt cagcccctcg gatgtcagga
     3121 acgtcggcat cttcacggag ccggcgccgg acgccttcag ctcggtcttc gtgccgcagc
     3181 cggcgaggga gccgacggcg gcgacgctga gtgcgccgaa cccgacgtac ttcaggaacg
     3241 tccgcctgtc ggtggcgatc tctctgattg acatttccga tcctctctga gggaaatcag
     3301 gccggtggcc tcgggtgttc ttcggctggg gtcagccctt gacggcgccg gtgaggacgc
     3361 ccttggtgaa gtacttctgc aggaaggggt acacgcacag gatcggcacc aggctgatca
     3421 cgacgacggc catctggatg gcctggctgg gagcggcgat ctcggcgccg gtgttggcgt
     3481 cgatggatga gccgagcagc acgtactgcc gcagcaccat ggccagcggc cacttgtcgc
     3541 tgtcgacgta gagcatggcg ttgaagaaca tgttccagta gccgacggcg tagaacaggc
     3601 cgatcacggc gatcaccgcc ttcgacagcg gcagcatcac gttccacagg atgcggatgt
     3661 cgcccgcgcc gtccagccgc gccgcctcga cgagctcgga ggggacgttc atgaagaagg
     3721 agcggatgat gatcaggttg aacgccgaga gcgccgtggg caggatgagc gccgcgaagg
     3781 tgccgagcag cccgagctcc ttgaccacga gaaacgtggg gatgatgccg ggtgagaaca
     3841 gcatggtcag cagggcggtc atcaggaaga agttgccgcc catgatccgt cgcgacagcc
     3901 cgtacgccat catcaccgtg agggtcaacg agagcgcggt gccgacgagc gtgatgccga
     3961 tgctgcggaa cagtgcctcg gtgacgatgc cgccggagag gatggtccga tacgcctcga
     4021 gcgtgggatg catggggatc agcacgaagc cgttgttcgc ggcgatctcc tgctgcgtgg
     4081 acaggctcgt gccgatcacg accaggaacg ggtagatcac cgccacgcac accagcagca
     4141 ggacgagtcc cttcagcccc tgcatcgcga agctgggctt ctccatccac accgggcggg
     4201 tgcgggcccg gtagtcgcgg gccctctgcc ttccgagacg gaccaccgtc gtgctctcag
     4261 tgctcacggg agtacacccc atcctgtccg aacatgtggg cgaccttgtt cgcgccgagc
     4321 accaggaaga gaccgacgat gcccttcacg aggcccaccg ccgccgaggt gccccattgg
     4381 ccgtcgacga tgccgtggaa gtagacgtag gtgtccagta cctcgcccgc ctgtgcgccg
     4441 acgttgttgc gctgcaggat gatctgctcg aacccgacgg acagcgccgt gccgaggttg
     4501 aggatcagca gcaggatgac gatgggcatg atgccaggga tcgtcaccgc ccacgtgcgc
     4561 cgccacgggc cggcaccgtc gatggccgcc gcctcgtaga ggctgtcgtc gatgctggcc
     4621 agcgcggcga ggaacatgat cgtgccccag cccgcgtcct tccagatggt ctggacggtg
     4681 acgagcagcg ggaacgtggc ggggtcggtc atcgggctga tccgccccat cccgagggag
     4741 tcgaggaagt tcgggatgag tccggtgggt ccgaggatct gcgtgaacag agagacgatg
     4801 atcacccagc ccaggaagtg cggcaggtag atcacgctct gcacgaactt cttgagcttg
     4861 ggcgacatga tgctgttcag cagcagcgcc agggcgatcg gcacggggaa gaacagcacg
     4921 agctgcatca ccatgatcac gagcgtgttg cgcagggcat accagaacgc cgggtcggag
     4981 aacgcgacgg cgaagttgtc caggccgacc cacacgctgt cgacgaagcc gaggtagggc
     5041 tggtagtcct cgaacgcgat gatgttgccg gggatgggca gccagtggaa gaccacgaag
     5101 tacaggaacc cgggcatcac cagcaggagc atgacgcggt cccgccggaa gcgggcggcg
     5161 aagctcagtc gcgtgcgacc cttgcggtcg agccggcgcg cgtgccccgt gaccgggcgc
     5221 gccggctcgt ccgcggcggc cttcggcctg ctcgtgacgg gtgtcgagac caccgcatcc
     5281 tccgaacgtc gttgtggatg aaacgtcgcc ttagtaagcg attgctatcg caaggtagcc
     5341 cgtcggtgat cgcattgtca acgtcagtcg tgaggtttcc ggagatctag ggcgattcgg
     5401 ccatcctctc tggcatccga agcggaaagc atgatagaaa tcgcttactg tctgtttcca
     5461 tcgttctcgg agatgttcat gagctggcac ccccgcgtcg cgttcgcgat gaaccgcgac
     5521 gaccacctcg cggcgttccc cgcgtcgctc ctcgagcgcc tgggctcgat cgccgaggtg
     5581 atcactcccc tgccgatgga ggagttcgac tccgccgcgt cccgcgagct cctcgcggag
     5641 accgaggtgc tcatcacggg ctggggtgcg ccgcgcttcg ggctcgcgac gctcgcacag
     5701 gccccgcacc tgcgggcgat cgtgcacgcg gcggggaccg tgcgcacctt cctcgaccct
     5761 gccgtgtacg acaggggcat ccgcgtgagc acctgtgcca gcgcgaacgc catccccgtg
     5821 gccgagttca cgctcgcgag catcgtcttc ggactcaagc gcgcctcccg cttcgccgag
     5881 cgactccgtc gcaccaaggc cgtgcgcgac accgccggat tcccgcccat cggcacgaac
     5941 ggcgtgaccg tcggcctgat cggcgcctcg cgagtggggc gggagaccct gcggatgctg
     6001 cgcacgctcg acgtccgcat cctgctgcac gacccctacc tctcgcccgc ctccgccgcc
     6061 gagctcggcg ccgagctggt cggcctggac gagctgtgcc ggcgcagcga tgcggtgagc
     6121 gtgcacgcgc ccctcaccgc acagaccgtc ggcatgatcg gcacgcacga gctgtcgctg
     6181 atgcgcgacg gcaccgtgct cgtgaacacc gcgcgaggtg cgatcatcga caccgccgca
     6241 ctcaccacgg agctggtcgc cggccgactg gacgcgtacc tcgacgtcgt gcaccccgag
     6301 ccgctgcccg ccgattcacc gctgtacgga ctgccgaacg tcgtcatcac cccgcacatc
     6361 gccggcgcgc tcggcaacga ggtcgtgcgg ctcggtgagc tcgccgtcga cgaggtcgcc
     6421 cgcttcgcgg cgagtgatac cttcgaccat gaagtgcggt ccgccgacct ggcgatcacc
     6481 gcatgacgga caacgggatg aggcgccgtg tcttcgacat ctgagcagac tcgcgtccgg
     6541 gggcttcgtc ggccggccac agtcagcgac gtcgccgccg ccgcgcaggt ctccaccgcg
     6601 accgtctcgc gagtgctgaa cggcaactat cccgtcgccg cagccacccg cgagcgggtc
     6661 atgcgagccg tgtccaagct cggatacgcc gccaacgcga acgcgcgcgc gctgacgcag
     6721 tccgacacca agacgatcgg gatcatcgtg cccgagctcg tcgacccgtt cttcggctac
     6781 atggtgcgcg ggctggagcg cgccacccag gagtccggac gcctcgcgat catcgcgacg
     6841 acgtccttcc gccccggcgc cgagatcgct ctgatcgacc ggatgcgcga gcgccgcgtc
     6901 gacgccgtga tcgtcgtcag cggcggcagc agcgaccccg cgtaccggcg ccagttggct
     6961 cagcgcgccg atgctctcgc cgccatgggg agtcggctcg tgctgtgcgc gcatccgacg
     7021 ctggacctgc cgtccgcggc acgctccgtg gcatacgaca ccgagggcgg cgccttctcc
     7081 gtcacggagc acctgatcgc gaacgggcac cggcgcatcg cgatggtcgg cggtgagagc
     7141 cacctcccga ccatcggtct gcgccgcgac ggctacctgc gcgctctcgc gtcgcggggc
     7201 atcgacatcg acgaggatct gatccgcatc gagggcttcg gccgcgacgt cggctacgcc
     7261 agcacgcgtg ccctgatgga ggagcatccg gacgtcacgg cgatcttcgc cgtcaacgac
     7321 accatcgcct cgggcgtgta cgacgccctg ggcgacgcgg gtcttcgcat ccccacggat
     7381 gtgtcggtgg tcgcctacga cgacaccccg ctcgccgcga acctgtcacc caagctcacg
     7441 acggtgcacg ttccgctgga gcagctcggc caggaggcgc tgcgcgccgc gatcgacgag
     7501 ccggacgcgt tctcccggac gcccgagcag ggcctcacgc tcggcaccta cctcgtgcac
     7561 cgcgactccg tcgccatgcc gccgcgctga gccgacggcg ccgccccgat ccgttcggga
     7621 ggggaaaacg cggacgggag gagtcccggc ccgggaatct cctcccgagc gcgcgaactc
     7681 ctcccgaacg tgcggcgaag cgcgatacgg aggcggctca gaggttcgcc tccgcgtaga
     7741 tgcgcagggc gtcgcggacg aactcggcgc cggcggagcc gccgtcgctc gtcgcatagt
     7801 tggcgccgaa gcgcgggtcg gcgacgtaca tctcaccgag gcccatcacg tagcccttca
     7861 cgtcgccgcc cggggcggat gccggggtgc ccggcacgcc cgtgagccac tcgacgtgac
     7921 ggcgggcgat ggcctgcgcc tcatcggagt ccggggcgat gccgctctcg gcggcggcga
     7981 tccagtcgcg gttcaggtcc gcgacgcgct gcttccaggc ggcgcggtcg tcgtcggaga
     8041 ggccgcgcca ccagcggtcg ccgtcggcgt acgcctggcg accccagcgc tcctcgacct
     8101 cctccttgta ctgcgtgtgg tcgaagccgt cgaacatgtt ctcggccatg agagtcttcc
     8161 cttctgtcaa tgcggtgatg gtcgattcga cggacgcgat ctgccgcccg atccgctgct
     8221 gctcctgacg gagcagctcg aggtgggtct gcagagcgga tgcctcggcc atctgggtcg
     8281 ctgagcccgt cgaagcgccg agcacctcgg cgatctgcgg cagaccgagc ccgagctcgc
     8341 gcagcagcag gatgcgctgc aggcgcacga gcgcggcctc gtcgtagtgc cgatagccgt
     8401 tggcggcgat gcgcgagggg gcgagcagac cgatgtcgtc gtaatgacgc agcgtcctgc
     8461 tcgtcgtccc cgcgaggcgg gcgatctcct ggatcgacca gtccatggca tccgctcctc
     8521 tcgttcgtcg atatgtccac ggtagaagtt gacgttacgt caaggtcaag cagaacgttc
     8581 ctgattcttt gctgaacccc ttgacgaact cgagatatat cgtgttactg tctcaatacg
     8641 cgatatatct cgatatcgcc caaccaggaa gagaacgaca atgaccgaga agtggctcat
     8701 cgcccccggc gaggaacgcg tcatcgacat cgcctccgcg tcccggctca aggtcgggct
     8761 cgtcggcgga caggtcgacg tggtcgcaca cgacgaaccc ggcatccgca tcgaggtgca
     8821 cggcgtcacc atcaaggacc tgcgcgtcga gtccgacggc acccagatcg agatcgacca
     8881 ccctcagatc gggtgggaca acttcctcga ggtgttccgc aacttcggcg ccggcggccc
     8941 caaggccgag atcagcgtcg ccgtgccgcg tgcgatcgca ctcacgctcg gcgtggtcag
     9001 tgcgggcgcg ctggtgtccg gcatccgcaa cgacgtcaag ctcaacaccg tctccggcga
     9061 catcatcgcc gacgacgtga ccggcgacct ctcggtgaac tccgtgtccg gtgacgtgca
     9121 gctgcgtggc ctgaccggct ccgtggtcgc gaacagcgtc tcgggcgacg tcgccgtgac
     9181 cggctcgctg cgcaaagctg tcatcgacac cgtctccggt gacgtgctga tcgacgcgat
     9241 cggcgacgtg aacaccatga acgtcaacac cgtctctggc ggcgtgaccg tgcgcctgga
     9301 cgagaccctg ccggcgaact acgccctgcg cagcatgagc ggcaagctca tggtcgacgg
     9361 catccagcgc tccagcggcg ggccgagcaa ctacaccggg cagatgggcg agctcgccgg
     9421 cagcttcgtc gacgtgcgcg cgaacactgt ctcgggcggc atcacggtgc tgcgccaggc
     9481 gcgtccgacc gtcgccgacg acccggaatg ggaggagtga tggcaccggc agtcttctcg
     9541 cacggcgacc tgcggctgta cctgctgtcg ctgctgaacg aggcgccgca gcacggctac
     9601 ggcatcatcc aggcgctgac cgaccgcacc ggcggcacct atacgcccag cgccggcacg
     9661 atctatccgc ggctggcgaa gctggaggag gagggcctgg tcaccaagac cgtcgagggc
     9721 cgcaccacca tctacgccat cacggatgcc ggtcgcgccg agctcgaatc gcgctcgtcc
     9781 gacctggagg gcatcgagga gggcctctcc gactcggtgc gcctcatcgc cagcgaggtg
     9841 cgtcagagcg tgaaggaggc gatgcgcagc ctgcgcgccg acctcgccac cgccgcacag
     9901 gaggagcgcc gcgccgccaa gaagcagccc cgcgcggaag ggctgggcga cgagcgcgtc
     9961 acgagccgcg agaacctgca gcgggcggat gccgcgatca acgcgttccg cgcccaggtg
    10021 cgcacc
//
LOCUS       DATMSX010000002         7468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_132744,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000002 DATMSX010000000
VERSION     DATMSX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132744"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VN041_00055"
     CDS             <1..481
                     /locus_tag="VN041_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27470.1"
                     /translation="AQPAPDASTTPAPAVPDESAPVFTKTPAQMASKGLSFTGLKSIS
                     LVTVPTVDGDRIRVLKISADTITITGFSLSVRPPEHDGLVTDADTMSLKGDVTVYLGS
                     LTATGQDGQTLLTFGLDTPPSLDDVEPGLLHVQMGLVGTIADSISYTNTDQRMVAKK"
     gene            519..1094
                     /locus_tag="VN041_00060"
     CDS             519..1094
                     /locus_tag="VN041_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27471.1"
                     /translation="MSLQVRPATEFDDVAAVVGPRNPASNVCFCLNYRLGSTEARKLR
                     EPDRSAKVRELCRHDPPPGVIAYQGDQPVGWAALHPRRNTSFARNRLIPHVDDLDVWS
                     LWCFRVRPGHRGQGVMHALLDGAVSYARERGAPAIEGYPVDNGGDAVNLTMAYVGTRA
                     LFESAGFAKAADTGSVLDGFPRVLMRLDLSA"
     gene            complement(1084..1566)
                     /locus_tag="VN041_00065"
     CDS             complement(1084..1566)
                     /locus_tag="VN041_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU27472.1"
                     /translation="MATPDFVLALREHIGHAPLPLVGTTAVVFRDEKVLLGKRADNGA
                     WQCIAGIVEPGEEPADAAARECLEEAGVVVSVDRLALVHQLPRITYANGDQVDYTDIV
                     FRCTWVSGDPHPADGELTEVGFYDLAAMGDVDDAHVRKIALAMAEDDPAHFRGGRVKR
                     "
     gene            1640..2230
                     /locus_tag="VN041_00070"
     CDS             1640..2230
                     /locus_tag="VN041_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU27473.1"
                     /translation="MSTDSEIIERSLDEPEAFSGIFERHVRPVGGYIRRRIGADAVDD
                     ALSETFLVAFRRRSSFDSTVGSALPWLLGIATRVVKRHGADEARQWRSFEAAAGAAPL
                     SDGGHDASDERMDAGAALRALAPRIAALAPRDRDALLLYAWGDLTYEQVALALGVPVG
                     TVRSRLNRVRRRLAPPGSHSATRLTWMAKEASDVAH"
     gene            2217..3242
                     /locus_tag="VN041_00075"
     CDS             2217..3242
                     /locus_tag="VN041_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27474.1"
                     /translation="MSLIEQVRRIGADDTDITEENVRTARLALLRETNRARGGRRTRA
                     HHRTLWAGIGVGGLVAAAAVVAMVVGSVLSPSATPSASAAEVLDAAAAKTSGATVLHP
                     APGQYIRISETSTQTLGWHEDPSAPDGGWWESAASDTSAKVRIRSSLYVPADRRHDWI
                     QTWGERFTVLSISGPDAVAAGKVLESEMRDQKTEVLPAGRYADADAPEDGGVGVAPPR
                     NGMQCFYDSMPRDPKKLIAWLDAFEPASDFGCGSPDLADPVGFNLAPADLRETMFHAL
                     ALVDGAKIVKAEGDVTTISFPEASGSSWRDTIDVDTARGLIVGRGYGSSDSWSSRVDM
                     SIVDEAP"
     gene            3310..6828
                     /gene="dnaE"
                     /locus_tag="VN041_00080"
     CDS             3310..6828
                     /gene="dnaE"
                     /locus_tag="VN041_00080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWU27475.1"
                     /translation="MASDSFVHLHVHSEYSMLDGAAKIGAMTQAAAEYGMPAIAVTDH
                     GNTFAAFEFYNAAKNAGIKPIIGLEAYMTPGTHRSDKTRVSWGSPDQKSDDVSGSGAY
                     THMTMWSETTQGMHNLFRISSRSSMEGYYFKPRMDRELLQTYGKGLIATTGCPSGEVQ
                     TRLRLGQYDAARAAAAEFQDIFGKENFFAEIMDHGLSIERRVMTDLLRLAKDLNIPLV
                     ATNDSHYTHQHEADAHEALLCVQSGSTMDDPNRFKFDGDGYYIKTAQEMRQLFRDHPE
                     ACDNTLLIAERCEVEFDTSANYMPKFPVPDGETEDSWLVKEVEAGLHYRYPGGIPDKV
                     RKQAEYELGVILQMGFPGYFLVVADFINWAKDNGIRVGPGRGSGAGSMVAYAMRITDL
                     DPLEHGLIFERFLNPDRVSMPDFDVDFDDRRRGEVIEYVTEKYGSERVSQIVTYGTIK
                     SKQALKDAGRVLGFPFSMGDRLTKAMPPAVMGKDMPLSGMFDSAHPRYKEASEFRALI
                     ETDPEAKTVFDRALGLEGLKRQWGVHAAGVIMSSHPLLDIIPIMKREQDGQIVTQFDY
                     PSCESLGLIKMDFLGLRNLTIISDALDNIRTNRGEELDLEHLALDDRPVYDLLARGDT
                     LGVFQLDSPPLRSLMRLMKPDNFGDISALIALYRPGPMGANSHTNYALRKNGLQEITP
                     IHPELEEPLKDILEESYGLIIYQEQVMAIAQKVAGFSLGQADILRRAMGKKKKSELDK
                     QYEGFSGGMKERGYGEGAIKALWDILLPFSDYAFNKAHSAAYGLVSYWTAYLKAHYPA
                     EYMAALLTSVGDSKDKMALYLNECRRMGIKVLPPDVSESINFFAAVGEDIRFGLGAVR
                     NVGSNVVDGIIQARKDGKFTSFHDFLGKVPLHVANKRTVESLIKAGAFDSMGDTRRAL
                     LEIHEDAVEASVDRKRNEAQGAIGFDFDSLYDDMEEAAPAKVPERPEWTKKDKLAFER
                     EMLGLYVSDHPLAGLEMPLAKHASISIHDLLTSEDLQDGDQVTVAGLVTSVQHRVAKA
                     SGNPYGMITVEDFNGEVTVMFMGKTYTEYQHVLQQDAILAVRGRVSRRDDGLNLHAQS
                     AFAPDVGSFDAAGPLSLQVAEQRATERTMIDLAEVLRRHAGETEVLLRVHRGGSAKVF
                     EVPMPVTVSADLFGDLKSLLGPTCLG"
     gene            6928..>7468
                     /locus_tag="VN041_00085"
     CDS             6928..>7468
                     /locus_tag="VN041_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27476.1"
                     /translation="MSTEHIGTGPEGVTGGSFSEFDGEEWRTDAPAADRDAAADEPAS
                     EDDDAVYYEEQVEPEYGILGFTLRELIIVGAWVVVFVVSFFPVFGGGGSVWTRGIDWI
                     LTIGLPTVAVFLIALRRFSPEGIRRVGSLGIDQFASVASSVAAVAWAQLLWLQSAASV
                     DSGAFLVGWVPIVAQLATLA"
BASE COUNT         1261 a         2507 c         2543 g         1157 t
ORIGIN      
        1 ggcgcagccc gcgccggacg cgagcacgac gccggcgccc gcggtgccgg acgagagcgc
       61 gccggtcttc accaagaccc cggcgcagat ggcatccaag gggctgtcct tcaccgggct
      121 gaagtcgatc agcctggtca ccgtcccgac cgtcgacgga gaccgcatcc gtgtgctgaa
      181 gatcagcgcg gacacgatca cgatcaccgg cttctcgctc tccgtgcgcc cgccggagca
      241 cgacgggctg gtcaccgacg ccgacaccat gtcactcaag ggcgacgtca cggtgtacct
      301 cgggtcgctg accgcgaccg gccaggacgg ccagacgctg ctcaccttcg ggctggacac
      361 gccgccgtcg ctggacgacg tcgaacccgg cctgctgcac gtgcagatgg gcctggtggg
      421 aacgatcgcg gactccatca gctacacgaa caccgaccag cggatggtcg cgaagaagtg
      481 agccggcgga tgtcggtggc gtgcgccaga ctcgcagtat gagtcttcag gtgcgcccgg
      541 cgacggagtt cgacgacgtc gccgccgtcg tcggccccag gaaccccgca tccaacgtgt
      601 gcttctgcct gaactaccgg ctgggcagca ccgaggccag gaagcttcgc gaaccggatc
      661 ggtccgcgaa ggtcagggag ctctgccggc acgatccgcc tccgggagtg atcgcctatc
      721 agggggacca gccggtcggc tgggctgcgc tgcacccccg gcgcaacacg agcttcgccc
      781 gcaaccgcct gatcccgcac gtcgacgacc tggacgtgtg gtcgctgtgg tgcttccgcg
      841 tgcgacccgg gcatcgcggc cagggcgtga tgcatgcgct gctcgacggt gcggtgtcct
      901 atgcgcggga acgcggtgcg cccgcgatcg agggctatcc ggtcgacaac ggcggcgatg
      961 cggtgaacct caccatggcg tacgtgggca cccgcgccct gttcgagagc gcgggattcg
     1021 cgaaggccgc cgacaccgga tcggtgctgg acggcttccc gcgggtactg atgcggctgg
     1081 atctcagcgc ttgacgcgcc cgccgcggaa gtgcgcggga tcgtcctctg ccatcgccag
     1141 tgcgatcttg cgcacatgcg cgtcgtcgac gtcgcccatg gcggcgagat cgtagaagcc
     1201 gacctcggtg agctcgccgt cggccggatg cgggtcgccg gacacccacg tgcaccggaa
     1261 cacgatgtcg gtgtagtcca cctggtcgcc gttggcgtac gtgatcctcg gcagctgatg
     1321 caccagggcc agtcgatcca ccgacacgac gaccccggcc tcctccaggc actcccgtgc
     1381 ggcggcatcc gctggttcct cgccgggctc cacgatgccg gcgatgcact gccaggcgcc
     1441 gttgtcggcg cgcttgccga gcagcacctt ctcgtcgcgg aacaccaccg ctgtggtgcc
     1501 gacgaggggg agcggagcat gtccgatgtg ctcgcgcagc gcgaggacga agtcgggagt
     1561 ggccatgggg tcagcgtatc cggacggggc gatgaaaaga tttcgtgaac cggaagtcag
     1621 gacacgggca atacccattg tgagcacaga cagcgagatc atcgagcggt cgctcgacga
     1681 gcccgaggcg ttctcgggca tcttcgaacg gcacgtcaga ccggtcggcg gctacatccg
     1741 ccgccggatc ggagcggatg ccgtcgacga cgcgttgagt gagacgttcc tcgtggcgtt
     1801 tcgccggcgt tcgtcgttcg acagcacggt ggggtccgcc ctcccctggc tgctcggcat
     1861 cgcgacgcgt gtggtgaaga ggcacggggc cgacgaggcc aggcagtggc gctcgttcga
     1921 ggcggctgcc ggcgccgcgc ccctgagcga cggaggtcac gatgcctccg atgagcggat
     1981 ggacgcgggg gcggcgttgc gggcgctcgc cccgcggatc gccgccctcg ccccgcgcga
     2041 tcgggatgcc ttgctgctgt acgcgtgggg tgatctgaca tacgagcagg tcgcgctcgc
     2101 cttgggtgtt cccgtcggaa ccgtgagatc gcgcctcaat cgcgtccggc gcaggctcgc
     2161 acctccgggt tcgcattccg cgacccgatt gacctggatg gcgaaggagg cgagcgatgt
     2221 cgctcattga gcaggtgcgc aggatcggtg cggatgacac cgacatcacc gaggagaacg
     2281 ttcggacggc gcggctcgcg ctcctgcgcg agacgaaccg cgcgcgcggc gggcgacgga
     2341 ctcgggccca ccatcgaact ctgtgggcgg gtatcggggt gggcgggttg gtcgccgccg
     2401 ccgcggtggt cgccatggtc gtcggatccg tgctctcgcc gtccgcgaca ccgagcgcgt
     2461 cggccgcgga ggtgctggac gccgccgccg ccaagacctc gggcgcgaca gtgctgcacc
     2521 ccgcgcccgg acagtacatc cggatctcgg agacgtccac gcagaccctc gggtggcacg
     2581 aggatccgtc cgcaccggac gggggctggt gggagagcgc cgcgtcggac acctcagcga
     2641 aggtgcggat caggagctcg ctgtacgtgc cggcggaccg gcggcacgac tggatccaga
     2701 cgtggggcga gaggttcacg gtgctgagca tcagcggccc ggatgccgtg gcagcgggga
     2761 aggtgctcga gagcgagatg agggatcaga agaccgaggt gctgcccgcg ggcagatacg
     2821 ccgacgcgga tgcgccggag gacggcggcg tcggagtcgc tcctccccgg aacggcatgc
     2881 agtgcttcta cgactccatg ccccgcgacc cgaagaagct gatcgcctgg ctcgacgcct
     2941 tcgagccggc atccgatttc ggatgcggct ccccggacct cgccgacccg gtggggttca
     3001 acctcgctcc cgccgatctg cgggagacga tgttccacgc gcttgctctc gtcgacggcg
     3061 cgaagatcgt gaaggcggag ggcgatgtga ccacgatctc cttccctgag gcgtccggat
     3121 cgagctggcg cgacacgatc gacgtggaca cggccagggg actgatcgtc ggccgaggct
     3181 acggcagtag cgactcctgg agcagccgcg tcgacatgag catcgtcgac gaggcgccgt
     3241 aggcgcggca tccgtccttc gcggccccgg caccggactc ggtgtcgggg tcgcccgtag
     3301 actcgaagca tggcctccga ctccttcgtg cacctccatg tccacagcga gtactccatg
     3361 ctggacggtg cggcgaagat cggggcgatg acgcaggccg cggccgagta cgggatgcct
     3421 gcgatcgcgg tcaccgacca tgggaacacg ttcgcggcgt tcgagttcta caacgctgcc
     3481 aagaacgcgg gcatcaagcc gatcatcggt ctcgaggcgt acatgacgcc aggtacgcac
     3541 cgcagcgaca agacccgcgt gtcgtgggga tcgccggatc agaagagcga cgatgtctcc
     3601 ggatcgggcg catacaccca catgacgatg tggagcgaga cgacgcaggg catgcacaac
     3661 ctgttccgca tcagctcgcg atcgagcatg gagggctact acttcaagcc ccgcatggac
     3721 cgcgagctgc tgcagaccta cggcaagggg ctcatcgcca ccaccggatg cccgtcgggc
     3781 gaggtgcaga ccagactccg cctcgggcag tacgacgcgg cgcgggccgc tgcggcggag
     3841 ttccaagaca tcttcggcaa agagaacttc ttcgcggaga tcatggacca cgggctctcg
     3901 atcgagcgcc gcgtgatgac cgacctgctc cggctggcca aggacctgaa catcccgctg
     3961 gtcgcgacca acgactcgca ctacacgcac cagcacgagg ccgatgcgca tgaggcgctg
     4021 ctgtgcgtgc agtccggttc gaccatggac gacccgaacc ggttcaagtt cgacggcgac
     4081 gggtactaca tcaagaccgc gcaggagatg cgccagctgt tccgcgacca ccccgaggcg
     4141 tgcgacaaca cgctgctgat cgccgagcgc tgcgaggtcg agttcgacac ctcggcgaac
     4201 tacatgccga agttcccggt tcccgacgga gagaccgagg acagctggct ggtcaaggaa
     4261 gtcgaggcgg gactgcatta ccgatacccg ggcggcatcc ccgacaaggt ccgcaagcag
     4321 gcggagtacg aactcggcgt catcctgcag atgggcttcc ccggctactt cctcgtggtc
     4381 gcggacttca tcaactgggc caaggacaac ggcatccgtg tcggacccgg ccgaggctcg
     4441 ggcgccggtt cgatggtcgc ctacgcgatg aggatcaccg acctcgaccc gctcgagcac
     4501 ggcctcatct tcgagcgctt cctcaacccc gaccgcgtct cgatgcccga cttcgacgtc
     4561 gacttcgacg accgtcgtcg tggcgaggtc atcgagtacg tgaccgagaa gtacggttcc
     4621 gagcgcgtct cgcagatcgt cacctacggc acgatcaagt ccaagcaggc gctgaaggac
     4681 gccggtcgcg tgctcggctt cccgttcagc atgggcgacc gtctcaccaa ggcgatgcct
     4741 cccgccgtga tgggcaagga catgccgctg agcggcatgt tcgactccgc gcacccgcgc
     4801 tacaaggagg ccagcgagtt ccgggccctg atcgagaccg atcccgaggc gaaaacggtc
     4861 ttcgatcgcg cgctgggcct ggaagggctg aagcgccagt ggggtgtgca cgcggcgggt
     4921 gtgatcatgt cctcgcaccc cctgctcgac atcatcccga tcatgaagcg cgagcaggac
     4981 ggccagatcg tcacgcagtt cgactacccg tcctgtgaat cgctcggcct gatcaagatg
     5041 gacttcctgg ggctgcgcaa cctcacgatc atctccgacg cgctcgacaa catccgcacg
     5101 aacagggggg aggagctcga cctcgagcac ctcgcgctgg acgaccgccc tgtgtacgac
     5161 ctgctggccc gtggcgacac gctcggcgtg ttccagctcg acagccctcc gctgcgctcc
     5221 ctcatgcgtc tgatgaagcc cgacaacttc ggtgacatct cggcattgat cgcgctctac
     5281 cggccgggcc ccatgggtgc caactcgcac accaactatg cactgcgcaa gaacgggctg
     5341 caggagatca cgccgatcca cccggagctg gaggaaccgc tcaaggacat cctggaggag
     5401 tcctacggac tgatcatcta tcaggagcag gtcatggcca tcgcgcagaa ggtcgccggg
     5461 ttcagcctcg gccaggcaga catcctgcgt cgcgcgatgg gcaagaagaa gaagtccgag
     5521 ctggacaagc agtacgaggg cttctccggc ggcatgaagg agcgcggtta cggcgaaggc
     5581 gccatcaagg cgctgtggga catcctgctc cccttctcgg actacgcgtt caacaaagcc
     5641 cactcggccg catacgggct cgtctcgtac tggaccgcgt acctcaaggc gcactacccg
     5701 gctgagtaca tggccgcgct gctgaccagt gtcggcgact cgaaggacaa gatggcgctg
     5761 tacctcaacg agtgccgccg gatgggcatc aaggtgctcc cgccggacgt gtccgagtcc
     5821 atcaacttct tcgccgccgt cggcgaggac atccgcttcg gtctcggcgc cgtccgcaac
     5881 gtgggcagca acgtcgtcga cgggatcatc caggcccgca aggacgggaa gttcacgtcc
     5941 ttccacgact tcctcggcaa ggtgccgctg cacgtcgcga acaagcgcac ggtggagtcg
     6001 ctgatcaagg ccggcgcgtt cgactcgatg ggggacaccc gtcgtgccct gctcgagatc
     6061 cacgaggatg ccgtcgaggc ttccgtcgac cgcaagcgca acgaggcgca gggcgcgatc
     6121 ggcttcgact tcgacagcct ctacgacgac atggaggagg ccgccccggc caaggtcccg
     6181 gagcgtccgg agtggaccaa gaaggacaag ctcgcgttcg agcgcgagat gctgggcctg
     6241 tacgtctccg atcacccgct cgccgggctc gagatgccgc tggccaagca cgcgtcgatc
     6301 tccatccacg acctgctcac ctcggaggac ctgcaggacg gcgatcaggt gaccgtcgcc
     6361 ggtctggtca cgagcgtgca gcaccgcgtg gccaaggcca gcggcaaccc gtacggcatg
     6421 atcaccgtcg aggacttcaa cggtgaggtg acggtcatgt tcatgggcaa gacctacacc
     6481 gagtaccagc acgtgctgca gcaggatgcg atcctcgcgg tgcgtggacg ggtctcgcgt
     6541 cgcgacgacg gtctgaacct ccatgcgcag tcggcgttcg cgcccgatgt cggctccttc
     6601 gacgcggcgg ggccgctgtc gctccaggtc gccgagcagc gcgccaccga gcggaccatg
     6661 atcgacctcg cggaggtgct ccgccgccac gccggcgaga ccgaggtgct cctccgcgtg
     6721 catcgcggcg gatccgcgaa ggtgttcgag gtcccgatgc cggtgaccgt ctccgccgac
     6781 ctgttcggcg acctgaagtc gctgctcggc ccgacctgcc tgggctgagc ggcggtcatg
     6841 cgatgacacc gctcccagct tcccccggca ggtctgggta ggatcgtgac tcgcgtgggg
     6901 acgcgaccac gacgaaagga tcgctcgatg agcaccgaac acatcggcac cgggcccgaa
     6961 ggcgtgaccg gcgggagctt ctcggagttc gacggagaag aatggcgcac ggacgccccg
     7021 gccgcagacc gggatgcggc ggcggatgag cccgcctccg aggacgatga cgcggtctac
     7081 tacgaggagc aggtggagcc ggagtacggc atcctcggct tcacgctccg cgagctgatc
     7141 atcgtcggcg cgtgggtcgt ggtcttcgtg gtgtccttct tcccggtgtt cggcggcggc
     7201 ggatcggtgt ggacccgcgg catcgactgg atcctcacga tcggcttgcc cacggtcgcc
     7261 gtgttcctga tcgcccttcg ccgcttctcg cccgagggga tccgacgcgt gggatcgctc
     7321 ggcatcgacc agttcgcctc cgtcgcatcc tccgtcgcgg ccgtcgcctg ggcccagctg
     7381 ctctggctgc agtccgcggc gagcgtcgac tccggcgcgt tcctggtcgg ctgggtgccg
     7441 atcgtggcgc agctggccac gctcgccc
//
LOCUS       DATMSX010000003         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123270,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000003 DATMSX010000000
VERSION     DATMSX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123270"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VN041_00090"
     CDS             <1..337
                     /locus_tag="VN041_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HWU27477.1"
                     /translation="RRLCTKCRRPFDGDAETLRQVGMPEEMIADAQVHTAVGCSACSN
                     TGYRGRVALHEVMTVTDEIEQRLVARATGADLRELALEQGMVALRDDGWVKVSDGITT
                     IEEVLRVSV"
     gene            complement(456..1694)
                     /locus_tag="VN041_00095"
     CDS             complement(456..1694)
                     /locus_tag="VN041_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU27478.1"
                     /translation="MATMQEWQYQAVDPATTQTVKGTMDATSDSAVTAKLRAQGLMPV
                     QVALVEKTGLRREITLPSLNRGVSAKTLAIFARQMSALINAGLPLMRTLNVLADQTSD
                     KRLKAVLTTVSADVESGSSLSASLQRHPLIFPPLMVSIVRVGESGGFLGAALDSIAEN
                     YRKQAELHGKIRSATTYPAVVLVIALIGVLVMVTFIVPVFEKMFAGLGGQLPLPTQIL
                     VTISHNMIWILPLLVVVIVIAWVWWVRNRYTDGYRRVVDPVKLRLPIFGPLITKMAVA
                     RFSRNLSMMLDAGVPIIQALSIVGQASNNWKIEQTVRDIQESIRGGRSFAVPLAKAGV
                     FPPMVSQMVAVGEESGTLAQMLGSIADFYEDEVETATEQLSSLIEPVLIVGLGVIIGG
                     MVISLYLPIFTLYGDIAKQG"
     gene            complement(1694..2827)
                     /locus_tag="VN041_00100"
     CDS             complement(1694..2827)
                     /locus_tag="VN041_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWU27479.1"
                     /translation="MTTLTELLRIGARSGASDVHITADAVPLMRVDGDLVGIPGHPDA
                     VSEDWVRAAAFGLMNTGQRADFERHHEVDLAHAAEGIGRFRVNVFRQLGAIAMALRFI
                     PDRVFTLEELGAPAIARDLALRPRGLVLLTGPTGSGKSTTLTAMVDIINSIRPVHIVT
                     IEDPIEFHHTSRRALIHQREVGSDTNSFAEALRRVLRQDPDVILVGELRDPESIMTAL
                     SAAETGHLVLSTLHTQGAAKSVNRIVDVFPADQQHQIRTQLGDTLQGVISQTLLPRAQ
                     SNGRTIATEVLINNPAVANLIREGQVAQLYTAMQAGATDGMHTLDQDLRRLVGEGAIS
                     AAVARTFATDPASLDGVFVRPTDLDAESWSHYAVEQQLEEWGS"
     gene            complement(2829..3554)
                     /locus_tag="VN041_00105"
     CDS             complement(2829..3554)
                     /locus_tag="VN041_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27480.1"
                     /translation="MTKQLITLIGALISAAVIVVAVLVGVVPLIGGVVSADGQREQVA
                     ATNAGFEKQIASLTTQKANLADIQGAVTQLRGQIPEQQLLNQVFERISRAATDAGVTV
                     TDASRGDLETFVARTGSKDAAAATAAPTPAPTPTPTTGTPIDAVKGTAGQANANAAAT
                     DAANGATPTTGAPAPAASTRQQVQLSIRVSAPDIASTFAFLDGLRAGPRAIAIDTATT
                     TESGGAFEMQITAIAFLQSSGGE"
     gene            complement(3554..4213)
                     /locus_tag="VN041_00110"
     CDS             complement(3554..4213)
                     /locus_tag="VN041_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27481.1"
                     /translation="MSRTAPASTLGIPRVNLLPPSEVARRERETLAGRWIWVVIAAVI
                     LSAALVAGAWLWSQLAQQQLAAEQNRTTTLVQQIGGLSEVSGALAADRDLRAYLAEAM
                     GSDIVWQDVRSSVESALPGGVALIGFELTPGAPAPTKLSDDDAKNAVGLEGTVTLDSP
                     NTLDIASIAERLRTVGAIMLSDANAMVESTTQDGHYTYTIDVSFDQRIYTGRFAAEGK
                     K"
     gene            complement(4213..>4491)
                     /gene="pilM"
                     /locus_tag="VN041_00115"
     CDS             complement(4213..>4491)
                     /gene="pilM"
                     /locus_tag="VN041_00115"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWU27482.1"
                     /translation="VGGRPVADLIARLRSTLSFYLNRPGATAIQQVLLTGGGAAVEGI
                     HTGLAERADAPVRVISLTDVAAVKDAAPVGDLAMNLVGTAGLALGKDS"
BASE COUNT          643 a         1461 c         1687 g          700 t
ORIGIN      
        1 ccgccggctg tgcacgaagt gccgccggcc gttcgacggc gacgccgaga cgctgcgcca
       61 ggtgggcatg ccggaggaga tgatcgcgga tgcccaggtg cacaccgccg tcggatgctc
      121 ggcctgctcg aacacgggct accgcggccg tgtggccctg cacgaggtga tgaccgtgac
      181 cgacgagatc gagcagcgcc tggtggcgcg cgcgaccggc gccgacctgc gcgagctcgc
      241 gctggagcag ggcatggtcg cgctgcgcga cgacggctgg gtgaaggtgt ccgacggcat
      301 caccacgatc gaagaggtgc tgcgcgtctc ggtgtgaggc gctggggcag cggcatccgc
      361 tcgactgcac cccatgccgt cgagtgcacc gcttgccgac gtcgccggcc ggtgcactcg
      421 acgggaagtg gtgttctcgc cgccgcggtc ccgcctcacc cctgcttcgc gatgtctccg
      481 tacagggtga agatcggcag gtagagcgag atcaccatgc cgccgatgat cacacccagg
      541 ccgacgatca gcaccggctc gatgagcgag gacagctgct cggtggcggt ctccacctcg
      601 tcctcgtaga agtcggcgat gctgccgagc atctgcgcga gggtgccgga ctcctcgccg
      661 acggcgacca tctgcgacac catcggcggg aagaccccgg ccttcgccag cggcacggcg
      721 aaggagcgac cgccgcggat cgactcctgg atgtcgcgca cggtctgctc gatcttccag
      781 ttgttcgacg cctggccgac gatcgacagc gcctggatga tcggcacgcc cgcatccagc
      841 atcatcgaca ggttccggct gaaccgggcg acggccatct tggtgatcag cggtccgaag
      901 atcggcaggc gcagcttgac cggatcgacc acgcgccggt agccgtcggt gtagcggttg
      961 cgcacccacc agacccaggc gatcacgatc accacgacca gcaggggcag gatccagatc
     1021 atgttgtgcg agatcgtcac caggatctgg gtgggcagcg gcagctggcc tcccagcccg
     1081 gcgaacatct tctcgaacac cgggacgatg aaggtcacca tcacgagcac gccgatcagg
     1141 gcgatgacga gcacgacggc ggggtaggtg gtcgccgagc ggatcttgcc gtgcagctcg
     1201 gcctgcttcc ggtagttctc ggcgatcgag tcgagggcgg cgccgaggaa tccgccggac
     1261 tcgccgacgc ggacgatcga gaccatcagc ggagggaaga tcagcggatg ccgctgcagc
     1321 gatgccgaca gcgaggagcc gctctcgaca tcggccgaga ccgtggtcag cacggccttg
     1381 agccgcttgt ccgaggtctg atcggcgagc acgttgaggg tgcgcatgag cggaaggccc
     1441 gcgttgatca gagcggacat ctgccgtgcg aagatcgcga gcgtcttcgc cgagacgccg
     1501 cggttcaggc tcggcagggt gatctcccgg cgcagaccgg tcttctccac cagggccacc
     1561 tgcacgggca tgagcccctg cgccctgagc ttggcggtga cggcgctgtc gctggtggca
     1621 tccatcgtgc ccttgaccgt ctgcgtggtg gcgggatcga ccgcctggta ctgccattcc
     1681 tgcatggtgg ccatcaggaa ccccactcct cgagctgctg ctcgaccgcg taatgcgacc
     1741 aggactcggc atccaggtcg gtcggccgca cgaacacgcc gtccagggat gccgggtcgg
     1801 tggcgaaggt gcgggccacg gcggccgaga tcgcgccctc ccccacaaga cgccgcagat
     1861 cctggtcgag ggtgtgcatg ccgtcggtgg ccccggcctg catggccgtg tacagctggg
     1921 cgacctggcc ctcgcggatg agattcgcga cggccgggtt gttgatgagc acctcggtgg
     1981 cgatcgtgcg gccgttcgac tgcgcgcgcg gcagcagcgt ctggctgatg acgccctgca
     2041 gggtgtcgcc cagctgcgtg cggatctggt gctgctggtc ggccgggaac acgtccacga
     2101 tgcggttgac cgacttcgcg gcgccctgcg tgtgcagggt ggagagcacc aggtgcccgg
     2161 tctccgcggc ggagagggcc gtcatgatcg actccgggtc gcggagctcg ccgaccagga
     2221 tcacgtcggg gtcctgccgc agcacgcggc gcagcgcctc ggcgaacgag ttcgtgtcgc
     2281 tgcccacctc gcgctggtgg atgagcgcgc ggcggctggt gtggtggaac tcgatcgggt
     2341 cctcgatggt gacgatgtgc accgggcgga tgctgttgat gatgtcgacc atggcggtga
     2401 gcgtggtcga cttgcccgag ccggtggggc cggtcagcag caccaggccg cgcgggcgga
     2461 gggccaggtc gcgggcgatg gcgggcgcgc cgagctcttc gagtgtgaag acccggtcgg
     2521 ggatgaagcg cagcgccatg gcgatggcgc cgagctgacg gaacacgttc acgcggaacc
     2581 ggccgatgcc ctcggccgcg tgggcgaggt cgacctcgtg gtgccgttcg aagtcggcgc
     2641 gctggccggt gttcatcagc ccgaaggcgg cggcgcgcac ccagtcctcg ctcacggcat
     2701 ccggatgccc ggggatgccg accaggtcgc cgtcgacccg catcaggggg acggcgtcgg
     2761 cggtgatgtg cacatcggat gcgccggagc gggcgccgat gcggagcagc tcggtgaggg
     2821 tggtcatctc actcgcctcc actgctctgg aggaacgcga tcgccgtgat ctgcatctcg
     2881 aacgcgccac ccgactccgt ggtggtcgcg gtgtcgatcg cgatggcgcg cgggcccgcg
     2941 cgcagtccgt cgaggaaggc gaacgtgctg gcgatgtcgg gggccgagac gcggatgctg
     3001 agctgcacct gctggcgggt ggacgctgca ggagcggggg cgcccgtcgt cggggtcgcc
     3061 ccgtttgcgg catccgtcgc cgcggcgttc gcgttcgcct gcccggcggt gcccttcacc
     3121 gcgtcgatcg gcgtcccggt cgtcggggtc ggcgtcggcg ccggggtggg tgctgcggtt
     3181 gcggccgcgg catccttcga cccggtgcgg gccacgaagg tctcgaggtc gccgcggctg
     3241 gcatccgtca cggtgacccc ggcgtcggtc gccgcgcggg agatgcgctc gaacacctgg
     3301 ttgaggagct gctgctcggg gatctgcccg cgcagctgcg tgacggcgcc ctggatgtcg
     3361 gccaggttcg ccttctgcgt ggtgagggag gcgatctgct tctcgaaccc cgcgttcgtc
     3421 gcggcgacct gttcgcgctg gccgtccgcg gagaccacgc cgccgatgag cgggacgacg
     3481 cccacgagca cggcgacgac gatcacggcc gccgagatga gcgccccgat gagggtgatc
     3541 agctgcttgg tcatcacttc ttcccctctg cggcgaatcg tccggtgtag atgcgctgat
     3601 cgaacgagac gtcgatcgtg taggtgtagt ggccgtcctg cgtggtgctc tcgaccatcg
     3661 cgttcgcgtc gctgagcatg atggcgccga cggtgcgcag ccgctcggcg atggacgcga
     3721 tgtcgagggt gttggggctg tcgagcgtga cggtgccttc gagacccacg gcattcttgg
     3781 cgtcgtcgtc actgagcttg gtgggcgcgg gtgcaccggg ggtgagctcg aacccgatca
     3841 gcgcgactcc gccggggaga gcggattcga cggaggagcg cacgtcctgc cagacgatgt
     3901 cggagcccat cgcctcggcg aggtaggcgc gcaggtcgcg gtccgcggcg agcgcgccgc
     3961 tcacctcgga cagtccgccg atctgctgga cgagggtcgt ggtgcggttc tgctcggcgg
     4021 cgagctgctg ctgcgccagc tggctccaca gccaggcgcc ggcgacgagc gccgccgaga
     4081 ggatgacagc ggcgatgacg acccagatcc agcggccggc gagggtctcc cgctcacggc
     4141 gcgcgacctc ggatggcggc agcaggttga cgcgggggat gccgagcgtc gaggcgggtg
     4201 cggtgcggga catcagctgt cctttccgag ggccaggccg gcggtgccca ccaggttcat
     4261 cgcgaggtcg ccgaccggtg cggcatcctt gaccgccgcg acatcggtga gcgagatgac
     4321 ccgcacgggt gcgtcggcgc gctccgcgag gccggtgtgg atgccttcga cggcggcgcc
     4381 ccctccggtc agcagcacct gctggatggc ggtggctccg gggcggttca gatagaagga
     4441 gagcgtgctg cgcaggcggg cgatcaggtc cgcgaccggg cggccgccga c
//
LOCUS       DATMSX010000004         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180107,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000004 DATMSX010000000
VERSION     DATMSX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180107"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VN041_00120"
     CDS             <1..1410
                     /locus_tag="VN041_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWU27483.1"
                     /translation="ALRDDLAARGIDRVVLSGMGGSSLAPEVIAGTAGVELTVLDSTA
                     PAQVLAALDGDLARTVLVVSSKSGTTLETDSHRRAFETAFEDLGLDPAEHIVVVTDPG
                     SALDASASEAGYRVFRADPNVGGRYSALTAFGLVPAGLAGVDISELLDEAEASLLEFA
                     IDSPENPALRLAAAIAGSSSDGTRRDKLGLVSDGTYIEGLPDWIEQLIAESTGKNGTG
                     ILPVVLLPVSPELESRPKDLQIVRFVEDADEFHLRERHPGEILVSGSLGALFLLWEHA
                     TAIAGHLLGINPFDQPDVESAKTAARGLLDARPEPSAPAFVQDGVEVRVSDPALAVSA
                     SIEGVLDALWASLPADGYVSIQAYVNRLELGQLQGLRELAAADSGRPATFGWGPRFLH
                     STGQYHKGGPAQGVFLQILERTDVDLEIPGRPFTFGQLIEAQAAGDAAVLVSHGRPVV
                     TLTVTDAVDDVLTLFTATQ"
     gene            1438..>2865
                     /gene="zwf"
                     /locus_tag="VN041_00125"
     CDS             1438..>2865
                     /gene="zwf"
                     /locus_tag="VN041_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWU27484.1"
                     /translation="MTAPVSRGRNPLRDPDDRRLNRIAGPSALVIFGVTGDLSRKKLM
                     PAVYDLANRGLLPPGFALVGFARRDWEDQDFAQVVYDAVKQHARTEFREETWQQLLQG
                     IRFVQGTFNDPDSFRRLRETVEKLDVERGTMGNHAYYLSIPPKAFPEVTKQLRESGLV
                     GEQHQDDTHWRRVVIEKPFGSDLETARELNRALEAAFPADSIFRIDHYLGKETVQNIL
                     ALRFANELYEPIWNRNYVDHVQITMAEDIGVGGRAGYYDGIGAARDVIQNHLLQLLAL
                     TAMEEPISLSAEHLRAEKEKVLAAVHVPEELSMATARGQYAGGWQGGEKVTGFLDEEG
                     MNPQSTTETYAAIKLEIDTRRWAGVPFYLRTGKRLGRRVTEIAVVFNRAPQHLFGRGN
                     ASELGQNALVIRVQPDEGVTIRFGSKVPGSGSNVRDVTMDFGYGHAFTEASPEAYERL
                     ILDVLLGDPPLFPRHEEVELSWKILD"
BASE COUNT          441 a         1050 c          947 g          427 t
ORIGIN      
        1 gcgctccgcg acgatctcgc ggcgcgcggc atcgaccggg tcgtgctctc cggcatgggc
       61 ggctcgtcgc tcgcacccga ggtgatcgcc ggcacggccg gcgtcgagct gacggtgctg
      121 gactccaccg ctccggcgca ggttctggcc gcgctcgacg gcgacctcgc ccgcaccgtg
      181 ctcgtggtgt cctccaagtc gggcaccacg ctcgagaccg attcgcaccg ccgcgcgttc
      241 gagaccgcgt tcgaggacct gggcctcgac cccgccgagc acatcgtcgt cgtcaccgac
      301 ccgggttccg ccctggacgc atcggcgtcg gaggcgggat accgggtgtt ccgggccgac
      361 ccgaacgtgg gcggacgata ctcggcactg accgccttcg gcctcgtccc cgccgggctc
      421 gccggcgtcg acatctccga gctgctggac gaggccgagg cctcgctgct ggagttcgcg
      481 atcgactccc cggagaaccc cgcgctgcgc ctggccgccg cgatcgccgg ctcgtcatca
      541 gacggcaccc ggcgcgacaa gctcggcctg gtgtctgatg gcacgtacat cgaaggactg
      601 cccgactgga tcgagcagct catcgcggag tccaccggca agaacggcac gggcatcctg
      661 cccgtcgtcc tgctgccggt ctcccccgag ctcgagtccc gcccgaagga cctgcagatc
      721 gtgcggttcg tggaggacgc cgacgaattc cacctgcggg agcgccaccc cggcgagatc
      781 ctggtcagcg gaagcctcgg cgcgctgttc ctgctctggg agcacgccac cgcgatcgcc
      841 ggtcatctgc tgggcatcaa ccccttcgac cagcccgacg tggagtcggc caagaccgcg
      901 gcacgcgggc tgctcgacgc gcgcccggag ccgagtgccc cggcgttcgt gcaggacggc
      961 gtggaggtcc gggtcagcga ccccgcgctg gcggtgtcgg catccatcga aggcgtgctc
     1021 gacgcactct gggcctcgct ccccgccgac gggtacgtct cgatccaggc gtacgtgaac
     1081 cgtctcgagc tggggcagct gcagggactg cgcgagctgg ccgccgccga ctccggtcgc
     1141 cccgccacct tcggctgggg accccggttc ctgcactcga cagggcagta ccacaagggc
     1201 gggcccgcac agggggtgtt cctgcagatc ctcgagcgca ccgacgtcga cctggagatc
     1261 cccggtcgcc ccttcacgtt cggtcagctg atcgaggcgc aggcggcggg cgacgccgcc
     1321 gtgctggtct cgcacggccg ccccgtcgtc acgctcaccg tcacggatgc cgtcgacgac
     1381 gtgctcaccc tcttcaccgc tactcagtga tcccagatct ggagatatcc cccaccgatg
     1441 accgctcccg tctcacgcgg acgcaacccg ctccgcgatc ccgatgaccg tcgtctcaac
     1501 cgcatcgcag gtcccagcgc actggtgatc ttcggcgtga caggtgacct gtcgcgcaag
     1561 aagctcatgc cggccgtgta cgacctcgcc aaccggggtc tgctgccgcc cggcttcgcc
     1621 ctggtcggct tcgcccggcg cgactgggag gaccaggact tcgcccaggt cgtctacgac
     1681 gccgtcaagc agcacgcccg caccgagttc cgcgaggaga cctggcagca gctgctgcag
     1741 ggcatccgct tcgtgcaggg cacgttcaac gacccggact ccttccggcg cctccgcgag
     1801 acggtcgaga agctcgacgt cgagcgcggc acgatgggca accacgccta ctacctctcc
     1861 atcccgccca aggcgttccc cgaggtgacg aagcagctgc gcgagtccgg tctggtcggc
     1921 gagcagcacc aggacgacac gcactggcgg cgggtggtca tcgagaagcc gttcggcagc
     1981 gacctggaga cggcgcgcga gctgaaccgc gctctcgagg ccgccttccc ggccgattcg
     2041 atcttccgca tcgaccacta cctcggcaag gagacggtcc agaacatcct tgcgctgcgc
     2101 ttcgcgaacg agctgtatga gccgatctgg aaccgcaact acgtcgatca tgtgcagatc
     2161 acgatggccg aggacatcgg cgtcggcggg cgtgcggggt actacgacgg catcggcgcc
     2221 gcgcgcgacg tgatccagaa ccacctgctg cagctgctcg cgctcaccgc gatggaggag
     2281 ccgatcagcc tgtccgccga gcacctgcgc gccgagaagg agaaggtcct cgcggccgtg
     2341 cacgtcccgg aggagctctc gatggccacc gcacgcggcc aatacgcggg cggatggcag
     2401 ggcggagaga aggtcaccgg cttcctggac gaggagggca tgaaccccca gtccaccacg
     2461 gagacctacg ccgcgatcaa gctcgagatc gacacccgcc gctgggcggg cgtgccgttc
     2521 tacctgcgca ccggcaagcg tctcggacgt cgagtgaccg agatcgccgt cgtgttcaac
     2581 cgcgcgccgc agcatctctt cggacgtggc aacgcgtcgg agctcgggca gaacgcgctc
     2641 gtgatccggg tgcagcccga cgagggtgtc acgatccgct tcggttccaa ggtgcccggt
     2701 agcggctcga acgtccgcga cgtcacgatg gacttcggct acggccacgc cttcaccgag
     2761 gcgagcccgg aggcctacga gcgcctgatc ctcgacgtgc ttctcggcga cccgccgctg
     2821 ttcccgcggc acgaggaggt cgagctctcc tggaagatcc tcgac
//
LOCUS       DATMSX010000005        18477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_85366,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000005 DATMSX010000000
VERSION     DATMSX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85366"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..843)
                     /locus_tag="VN041_00130"
     CDS             complement(181..843)
                     /locus_tag="VN041_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWU27485.1"
                     /translation="MDIINDLILQAVASPWIALILFAVAAIDGFFPPVPSETVLVAAA
                     AVAGTTGSTSVLLLCAAGALGAMVGDNIAYGIGRAVGTDRFRWMRRPRVASSLDLARR
                     TLSQGGAGLILGARYIPVGRVAVNMTAGALSYPWRRFLLLSAVGGALWAAYSVAIGLL
                     AGQWLKGQPLLSAVIGVVAALAIGVVVDRVAARRRRRTGEPPIPQNADAVAERCAPVM
                     GG"
     gene            995..2593
                     /locus_tag="VN041_00135"
     CDS             995..2593
                     /locus_tag="VN041_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWU27486.1"
                     /translation="MLAVHDLEIRVGARVLMSDVSFRVSDGDKIGLVGRNGAGKTTLT
                     KVLAGDVLPSEGKVTTSGELGYLPQDPRSGDPEMLARTRILDARGLGTLQLGISQASH
                     DMASEDPKIAERAMRKFANLTERFEALGGYAAEAEAASIANNLSLPDRILDQPLKTLS
                     GGQRRRIELARILFSDAGTMILDEPTNHLDADSVVWLREFLKGYKGGLIVISHDVELV
                     GETVNRVFYLDANRQVIDIYNMGWKHYQRQRVADEERRKKERANAEKKATQLQLQAAR
                     FGAKASKAAAAHQMVARAEKLLAGLDDVRQVDRVAKLRFPKPAPCGKTPLMASGLSKS
                     YGSLEIFTDVDLAIDRGSKVVVLGLNGAGKTTLLRILAGVDQPDTGQLEPGHGLKIGY
                     YAQEHENLDVKRSVLENMMSAAPDINETEARKVLGSFLFTGDDVLKPAGVLSGGEKTR
                     LSLATLVVSSANMLLLDEPTNNLDPASREEILGALAHYEGAVVLVSHDPGAVESLNPE
                     RVLILPDGVEDIWSREYQELIELA"
     gene            2792..3646
                     /locus_tag="VN041_00140"
     CDS             2792..3646
                     /locus_tag="VN041_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWU27487.1"
                     /translation="MDSRERAQRAMLTTRALGGVARVLTLQKQHVSRHDIGLALGVGL
                     LQRVRRGWVAEPAADRMLVDAARHGAVLSCVTQAKRRGLWVHEEEPGLHWGVTPGASG
                     GQPEHVHVHWARPLVPRHPDDLEDPVENVLALIADCEPHERALATWESALSKGLVTRE
                     MLERLPLKRVARELLAEAIPFADSGLETYLRMRLAWLGLPLTFQTWIAGHRVDLLIGD
                     RLVVQIDGATHTGAQRSEDIRHDAELRLMGYHVIRVGYRQVMEQWHVVQDLIMRAVAQ
                     GLHRRSRR"
     gene            complement(3720..4283)
                     /locus_tag="VN041_00145"
     CDS             complement(3720..4283)
                     /locus_tag="VN041_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HWU27488.1"
                     /translation="MLQTAGSRLADALRDADLGLRRGVVDLAPASDAWALAFADVHEV
                     LAATAPASVVAIEHIGSTAVPGLDAKPILDIGIAVRPGTPLSSLDGWLTGLGMLLRGD
                     ATDVRPDRMYGYELEPMIRLVNVHVVDADAEDLRRYRAFRDHLRANPADRDAYGALKH
                     ALAARHPDDRLAYIDGKADFITARRGD"
     gene            complement(4320..5135)
                     /locus_tag="VN041_00150"
     CDS             complement(4320..5135)
                     /locus_tag="VN041_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWU27489.1"
                     /translation="MRQRLLRWSGYLVVAILFAIACAFLSNWQFSRNDQKATEIALVE
                     RNYDAKPVALDDVVDAEGRLDARREWHPVQLRGEYLADQQLYVRNRPHGGTSAFEVLV
                     PFRQEDGRVLLIDRGWVPPREDSSPASTPAPPAGTVTVVVRMQPGEQLPASGRSAPEG
                     QVPTISLPAIADQVNPDLITGGYGLLASEKPAPSKALGGFDKPAEDPGPHLSYAIQWI
                     LFAVMGFIFIGYVIRTERIKAKEEAGERAPRPPRRRRDRDADDEDALLDAASR"
     gene            complement(5132..5329)
                     /locus_tag="VN041_00155"
     CDS             complement(5132..5329)
                     /locus_tag="VN041_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27490.1"
                     /translation="MTAQFECARADCRAAATHHIVWRNPRIHSEDRRKIWLACDEHVG
                     FLSDYLRTRDFPVEVFEGLPE"
     gene            complement(5326..5688)
                     /locus_tag="VN041_00160"
     CDS             complement(5326..5688)
                     /locus_tag="VN041_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HWU27491.1"
                     /translation="MKSTRHTPSVTSLPEAPQDEAKRRVRHYAITMGIRTACFILMAV
                     VQPIGWWTFVFAAGAIFLPYIAVVAANAGADSTPTTVESPVMELEADAPETAAEETPK
                     VITIHESGRPTPRRDDAS"
     gene            5812..6522
                     /gene="fabG"
                     /locus_tag="VN041_00165"
     CDS             5812..6522
                     /gene="fabG"
                     /locus_tag="VN041_00165"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWU27492.1"
                     /translation="MSTDRVVLVTGGNRGIGRAIAERFVRDGYRVAVTARSGEGPEGT
                     LTVRADVTDAAAVDAAFTEVEQKLGPVEVVVANAGITKDTLLMRMSEDDFDSVVSTNL
                     GGSFRVVKRASKGMLRARFGRVILISSVVGLYGSAGQTNYAASKSALVGFARSLTREL
                     GGRGITANVVAPGFIETDMTAELPEETQKQYKASIPAGRFATPDEVASAVVWLAGDDS
                     GYISGAVIPVDGGLGMGH"
     gene            complement(6551..7237)
                     /locus_tag="VN041_00170"
     CDS             complement(6551..7237)
                     /locus_tag="VN041_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU27493.1"
                     /translation="MDVILVPGLWLDAASWDAVLPALRAAGHRVHPLTMPGVGEPASE
                     IGIADWVAAVASRVDDFDAPVALVGHSGGGNVVWGATDARPERIARVVFVDSVPPAPG
                     RGISEFEVVDGVVPFPGWDRFPDEDVHDLDDATRARTAPLALSVPARVPTDPISLTDP
                     RRYTVPVTLLMGGLDQAAFEKAISEWGPYAEEFGAIEDAEVIKLGTGHWPQFSKPDVL
                     AQEIVAALDR"
     gene            complement(7305..7940)
                     /gene="serB"
                     /locus_tag="VN041_00175"
     CDS             complement(7305..7940)
                     /gene="serB"
                     /locus_tag="VN041_00175"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HWU27494.1"
                     /translation="MTTARFLVVLDADSTLIRNEVIELLADEAGRRAEVQAATEAAMR
                     GEVDFATSLRSRVAALKGVPTDAFERVRARIEPTPGVRDLTAAVHERGGIVGVVSGGF
                     HEILDHVAPELGVDRWRANRLATADGELIGEVDGAIVDAKAKADSLKQWAAELDVAPH
                     ATIAIGDGANDLLMMSAAGLGIAFNAKPAVRAAAALVVGPQDLSEVIPLLP"
     gene            complement(8006..9247)
                     /locus_tag="VN041_00180"
     CDS             complement(8006..9247)
                     /locus_tag="VN041_00180"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWU27495.1"
                     /translation="MSAPKKVFGIILAGGEGKRLMPLTADRAKPAVPFGGQYRLIDFA
                     ISNLINSGLRQVVVLTQYKSHSLDRHVSQNWRMSALLDSYVTSVPAQQRLGKRWFSGS
                     ADAILQSLNLILDEKPDIVVVIGADHVYRMDFQQMVDAHIASGASATVAGIRQPLALA
                     SQFGVIDADPETGRIREFLEKPTDPTGLSDSPNEVLVSMGNYVFDTDALIAAVEADGE
                     SASSGHDMGGDIVPYFVERGEAGYYDMKQNDVPGSSPRDRSYWRDVGTIDSYFDAHMD
                     LISTLPIFNLYNTAWPIRTQTVNTPPAKFVRDAVGRIGNTIDSIVSAGSVLSGTHLER
                     SVVGVGSLASGGSTITDSVLLDGVLVGAGARVHRAVLDKNVVLEDGATVGVDRERDLE
                     RGFTITDSGITVVGKGLRIAR"
     gene            9294..10478
                     /gene="glgA"
                     /locus_tag="VN041_00185"
     CDS             9294..10478
                     /gene="glgA"
                     /locus_tag="VN041_00185"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HWU27496.1"
                     /translation="MRVDIITKEYPPEIYGGAGVHVAELVAALRSSIDVQVRAFGSDR
                     DEPGTHAYRTPVELAGANAAVQTLGTDLAMVGDVAGADVVHSHTWYANFAGHLASQLH
                     GIPHVLTAHSLEPLRPWKAEQLGGGYAVSSGIEKLAYENAAAIVAVSAGMRADILRSY
                     PQVDPARVRVIHNGIDVERWRPIENPEFLASIGMDPARPSVVFVGRITRQKGLPYLLR
                     AAQLLPPEVQLVLCAGAPDTPEIMAEVQELVRLLQQTRQGVIWIERMLPRDELSAILT
                     AATTFVCPSVYEPLGIVNLEAMACGAAVVGTRTGGIPEVVADGVTGRIVPIEQLQDGT
                     GTPVDPEQFVSDLAAALTEVTGDPERAAEYGAAGRERARTEFSWGAIADTTRALYEEL
                     TA"
     gene            10497..11285
                     /locus_tag="VN041_00190"
     CDS             10497..11285
                     /locus_tag="VN041_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27497.1"
                     /translation="MSSALDFTDVVVRRDGRDIVDHVTWQVSEDQRWVVLGPNGAGKT
                     TLLQLADTLMHPTSGTVTILGETLGRTDVFEIRPRIGFASTAMARRIPRDETVLNTVM
                     TAAYSVMGRWNEDYEGIDERRARRVLADWKLDHLSERLFGTLSDGEQKRVQIARAVMT
                     DPELLLLDEPTASLDLGSREELLQLLSGYAASPTTPAMIMVTHHVEEIPVGFTHVMLL
                     REGGIVAQGPIAETLTAEALSEAFGMPITLSAEGGRYAARAVSA"
     gene            11395..11655
                     /locus_tag="VN041_00195"
     CDS             11395..11655
                     /locus_tag="VN041_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HWU27498.1"
                     /translation="MKTDIHPDYQAVVFRDLGSGETFLTRSTVTSDKTIELDGVEYPV
                     VDVEISSASHPFYTGKQRIMDSAGRVEKFNQRFKNFGGSSQK"
     gene            complement(11706..12011)
                     /locus_tag="VN041_00200"
     CDS             complement(11706..12011)
                     /locus_tag="VN041_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27499.1"
                     /translation="MTSPVATILGWSIIVLLIALPVLLGSIGLVILYSSKPLEQKYLV
                     SGGGVPGGYASGLEAVFAPTAHEAGVELDRMTKRTAPAPVAGDPPWAIDGARIRIDV"
     gene            complement(12071..12775)
                     /locus_tag="VN041_00205"
     CDS             complement(12071..12775)
                     /locus_tag="VN041_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWU27500.1"
                     /translation="MTSTTLPNWLTRVGVFDLETTGVDVTTDRIVTAHVGVLDRHGRE
                     IAARDWLADPGIPIPDGAAAVHGISTEHARTHGRPAGEVVAEISNALRVLFAQGMPIV
                     AYNASYDFSLLAHEAARHDVPVLIDPQPVIDPLVIDKAYDRYRPGKRTLSVVAEHYAV
                     PLDDAHEASADAVAAGRVALALAKEFALPGTAAELHTQQIGWARDQAESLTEYFIKVG
                     RLDPAEALDGSWPVRG"
     gene            12844..13467
                     /locus_tag="VN041_00210"
     CDS             12844..13467
                     /locus_tag="VN041_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWU27501.1"
                     /translation="MTWTVWLSLLLACVVISFTPGAGAINTMSNALTQGWRRSLWGVL
                     GQQAALVVHVVIVAAGVGLLVSRSQLLFDIIRYAGAAYLVYLGIRLILAKPQVETMAT
                     ASRSREHAWSIFRRGFWVNLLNPKAIVFFLAFVPQFIRLDQDPLPQYLILIGTVIVVD
                     VLVMWFFFAAAAKPFRRFTRDARGQRILNSIFGSLFIAVAGLLLFVH"
     gene            13603..14124
                     /locus_tag="VN041_00215"
     CDS             13603..14124
                     /locus_tag="VN041_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWU27502.1"
                     /translation="MTIRNDTIVIAGNIGNDPTKNETRNGKAVINFRVGTSHNYIDQR
                     TGEWVESPTSWYNVSAFGNIAEHAKASLHRGDPVIVVGRLRIRDWEAGDGKKGREAEI
                     TADAIGHDLNWGTSAFVRRQRATAEPVQAPQPSDADAPSDDAPSADEGDAWRIAGLEV
                     LAEDEASKEPAFT"
     gene            14177..14680
                     /locus_tag="VN041_00220"
     CDS             14177..14680
                     /locus_tag="VN041_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27503.1"
                     /translation="MLRGSSVSAPRIRAVATIAGATLIAAALASCSAPAEKPDPAASS
                     AVPSPSTSAAEAPVLVPDGTATDNLALFTAVTAQVWGSDDRARGRAYVDALVAAGFPK
                     DRMQVTGDQTTVGRAAESLQFSVAWGDEECLIGQVGTSTGDPVTAVMPQLASGRCLVG
                     RTRPIDW"
     gene            14789..16468
                     /gene="ettA"
                     /locus_tag="VN041_00225"
     CDS             14789..16468
                     /gene="ettA"
                     /locus_tag="VN041_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWU27504.1"
                     /translation="MAEYIYSMVRARKAVGDKLILDDVTMAFLPGAKIGMVGPNGAGK
                     STILKIMAGLDTPSNGEAKLTPGFSVGILMQEPELDESKTVLENIQDGVAIKPKLDRF
                     NEISALMADPDADFDALLAEMGVLQEEIDAADGWDLDSQLEQAMDALRTPSADAEIAP
                     LSGGEKRRVALAKLLLQKPDLLLLDEPTNHLDAESVLWLEQHLKDYKGAVIAVTHDRY
                     FLDHVAEWIAEVDRGRLLGYEGNYSTYLEQKAERLDIQGKKDAKLAKRLKDELEWVRS
                     SAKGRQTKSKARLARYEEMAAEAERTRKLDFEEIQIPAGPRLGNIVIEAKNLQKGFGD
                     RMLIDGLSFSLPPNGIVGVIGPNGVGKTTLFKTIVGLEPLDGGDLKVGETVKISYVDQ
                     TRANIDPNKTLWEVVSDGLDFITVGKIEIPSRAYVSKFGFKGPDQQKRAGVLSGGERN
                     RLNLALTLKEGGNLLLLDEPTNDLDVETLSSLENALLEFPGCAVVITHDRWFLDRIAT
                     HILAYEGTDENPSQWHWFEGNFEAYEKNKIDRMGLEAARPHRTTHRKLHRD"
     gene            16556..17074
                     /locus_tag="VN041_00230"
     CDS             16556..17074
                     /locus_tag="VN041_00230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWU27505.1"
                     /translation="MTARENAQVPVERILDDGRLHIPIQLRWGDLDAFNHVNNTAMLK
                     LLEEARVRAFWKPAPGEVAPPTAILDSGLSYGLLTLIARQEIEYLAPVPYQRHPLDIQ
                     MWFGKLGGSSIEVCYEVRPDAAAESDVLYARATTVIVLVDAESGRPVRLTPEMRSAWE
                     PLVGVPLEYRRH"
     gene            complement(17106..17990)
                     /locus_tag="VN041_00235"
     CDS             complement(17106..17990)
                     /locus_tag="VN041_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HWU27506.1"
                     /translation="MSTQENDRRSVSQLLQVLDLDASEARTTEDIFTGVSHAMPTGRV
                     YGGQVLSQSLVAAERTLPEDRIVHSMHGYFLRPGDATQGITFAVDRIHDGRSFSTRRV
                     QAFQGGVPIFSMISSFQDEDPGVDHAEPFPEDIPEPESLPSDDELIGAVHPITGRLLA
                     DRPADIRHVTGPLFLEVAGEHVPQQAVWMRMRAPMPDDPRVHRAALAYLSDMTIQESI
                     LRRHGITWQTPGLKVASLDHAMWWHRFGRVDEWMLYVQNSPNARGGRGLAQGRVYTRD
                     GVLMATVAQEIMVRVPEL"
     gene            18009..>18477
                     /locus_tag="VN041_00240"
     CDS             18009..>18477
                     /locus_tag="VN041_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27507.1"
                     /translation="MTRPLVLADADTARDVLTFSGRASKAGADGVRLQATGGVLAVTS
                     AALAPQGLFDQTPTILAMRIVRADPELSCDLVVDSLKEGRNPDELALPDTALSPAWAG
                     IAPPRGGWEHVADLPAGLIAERALWGISAVAHGSPDGAGEDAVRALRARIWGRS"
BASE COUNT         2954 a         6307 c         6443 g         2773 t
ORIGIN      
        1 gacggtgcac ggcgagggcg ggcgccgcgc acacgccggc gccgagaagg aacgcggcgg
       61 gaacagcgac cccgtacagc gcgatctgga tggggacgag cgtcgcatag agcgcgacca
      121 cgagagccca cagggcgacg atgcgccggg aggggcgttc acgggcggcg ctcatatccg
      181 tcatcctccc atcaccggcg cgcaccgttc tgcaacggca tccgcgttct gcgggatcgg
      241 cggttcgcct gtccgcctgc ggcgcctggc cgcgacccga tcgacgacga cgccgatcgc
      301 gagggcggcc accacaccga tcaccgcgct gagcagcggc tgccccttca gccactgccc
      361 ggccagcagt ccgatggcca cgctgtaggc cgcccagagg gcgccgccga ctgccgacag
      421 caggaggaag cgccgccagg ggtagctgag cgcgccggca gtcatgttca ccgccacgcg
      481 accgaccgga atgtagcgag cgccgaggat gaggcctgcg ccgccctggg acaacgtgcg
      541 tcgcgcgagg tcgagcgagg atgccacgcg cggccgccgc atccagcgga agcggtcggt
      601 gcccaccgcg cggccgatgc cgtaggcgat gttgtccccg accatcgcgc ccagcgcccc
      661 cgccgcgcac agcagcagga cgctcgtgct ccccgtcgtg ccggccacgg ctgcggcggc
      721 cacgagcacc gtctcgctcg gcaccggcgg gaagaagccg tcgatcgccg ccaccgcgaa
      781 gaggatcagg gcgatccacg gcgacgcgac cgcctgcagg atgaggtcgt tgatgatgtc
      841 cacctcacga cgctacgaag ccgccccggc cgggcgcatc cctctggagt atcgcccgcg
      901 tcatacctcg gagggggatc gtcccggccc gcgaactata ctgggaggct ggcccatcgg
      961 ccgacacccc cttttctacg aacggatacc cgctgtgctc gccgtgcatg acctcgagat
     1021 ccgcgtgggc gcacgcgtgc tcatgtcgga tgtgtccttc cgcgtctccg acggcgacaa
     1081 gatcggcctc gtgggccgca acggcgccgg gaagaccaca ctgaccaagg tgctcgcggg
     1141 agacgtgctg ccctcggagg gcaaggtcac cacctctggt gagctcggct acctgccgca
     1201 ggacccgcgc tcgggcgacc cggagatgct ggcgcgcacg cgcatcctcg acgcacgcgg
     1261 cctgggcacg ctgcagctgg gcatctcgca ggcctcgcac gacatggcat ccgaggaccc
     1321 gaagatcgcc gagcgcgcca tgcgcaagtt cgcgaacctc accgagcgct tcgaggcgct
     1381 cggaggctac gcggccgagg cggaggcggc gtccatcgcc aacaacctct cgctgcccga
     1441 ccgcatcctc gaccagcccc tgaagaccct ctccggtgga cagcggcgcc gcatcgagct
     1501 cgcgcgcatc ctgttctccg atgccggcac catgatcctc gacgagccga ccaaccacct
     1561 cgacgcggac agtgtcgtgt ggctgcgcga gttcctgaag ggctacaagg gcggcctgat
     1621 cgtgatcagc cacgacgtcg agctcgtcgg cgagaccgtg aaccgggtgt tctacctgga
     1681 cgcgaaccgt caggtgatcg acatctacaa catgggctgg aagcactacc agcgccagcg
     1741 cgtggccgat gaggagcgcc gcaagaagga gcgcgccaac gccgagaaga aggcgaccca
     1801 gctgcagctt caggcggcgc ggttcggggc caaggcgtcc aaggccgccg cagcgcacca
     1861 gatggtcgcg cgcgccgaga agctgctggc aggactggac gacgtgcgcc aggtcgaccg
     1921 ggtggccaag ctacggttcc cgaagccggc gccctgcggc aagacgccgc tgatggcatc
     1981 cggactgtcg aagtcgtacg gctcgctgga gatcttcacg gatgtcgacc tcgcgatcga
     2041 ccgcggctcc aaggtggtcg tgctggggct gaacggtgcg ggcaagacga cgctgctgcg
     2101 catcctcgcc ggggtggatc agccggacac cggtcagctc gagcccgggc acggactcaa
     2161 gatcggctac tacgcgcagg agcacgagaa cctcgatgtg aagcgctcgg tgctggagaa
     2221 catgatgtcg gccgcgcccg acatcaacga gaccgaggca cgcaaggtgc tgggctcgtt
     2281 cctgttcacc ggcgacgacg tgctcaagcc cgcgggcgtg ctctcgggcg gtgagaagac
     2341 ccggctctcg ctggcgaccc tggtcgtgtc gtcggcgaac atgctgctgc tcgacgagcc
     2401 caccaacaac ctcgatcccg cctcccgcga ggagatcctc ggggcgctgg cgcactacga
     2461 gggtgccgtg gtgctggtct cgcacgaccc cggcgccgtg gaatcgctga accccgagcg
     2521 cgtgctgatc ctgcccgacg gcgtcgagga catctggagc cgcgagtacc aggagctcat
     2581 cgagctcgcg tgacctgtcc gggcgatcgt cccggtcgcc gcactgcttc gcaattctgc
     2641 acaactccgc ggagatcgac tgaatctctg cggagttgtg catttctgcg tccctgcact
     2701 gccatccaca accagtggcc cacggtgagt tgtccacggg cgctgggatc gcgcgcgttt
     2761 cggatgctgc gcgacgggca tcctgtgcgt catggacagc agagaacgtg ctcagcgggc
     2821 gatgctgacc acgcgggcgc tcggcggcgt agcgagagtc ctgacgctgc agaagcagca
     2881 tgtcagtcgg catgacatcg gactcgcgct cggtgtcggg ctcctgcagc gggtgcgtcg
     2941 tgggtgggtg gcggagccgg cggcggacag gatgctggtg gatgctgcgc ggcacggtgc
     3001 ggtgctctcc tgcgtcacgc aggccaagcg gcgcggcctc tgggtgcacg aggaggagcc
     3061 gggactgcac tggggagtga cgccaggagc ttccggcggg cagcccgagc acgtgcatgt
     3121 gcactgggcg aggccgttgg tgccgcgaca tccggatgac ctggaggacc ctgtcgagaa
     3181 cgtcctcgcc ctcatagccg attgcgagcc tcatgaacgg gcgcttgcga cgtgggagtc
     3241 tgcgttgagc aaggggctcg tgacccggga gatgctggag cggctgccgc tcaagcgcgt
     3301 ggcccgtgag ctgctggccg aagccatccc gttcgcggac tccggtctgg agacctacct
     3361 gcgcatgcgc cttgcgtggc tgggactgcc actgaccttc cagacatgga tcgccgggca
     3421 tcgggtcgat ctgctgatcg gcgatcggct cgtcgtgcag atcgacggtg cgacgcacac
     3481 tggcgcccag cgcagcgaag acatccggca cgacgccgag ctgcggctca tgggctatca
     3541 cgtcatcagg gtcggctacc gccaggtgat ggagcagtgg cacgtcgttc aggacctgat
     3601 catgcgcgcc gtggcgcagg gactccatcg gaggtcgcgt cgctgactcg cagttctgca
     3661 caatctcgca gaacatggac gattccctgc ggaactgtgc cgaactgcgg agttgtgcgt
     3721 cagtcgccgc gtcgggcggt gatgaagtcg gccttgccgt cgatgtaggc gaggcggtcg
     3781 tcgggatgcc tggcggccag ggcgtgcttg agtgcgccgt aggcatcgcg gtcggcgggg
     3841 ttcgcgcgca ggtgatcgcg gaaggcgcga tagcggcgca ggtcctcggc gtccgcgtcg
     3901 acgacgtgca cgttgaccag gcggatcatg ggctccagct cgtagccgta catccgatcc
     3961 ggtcgcacgt cggtcgcgtc gccgcgcagc agcatgccga ggccggtcag ccagccgtcc
     4021 aatgacgaca ggggagtgcc tggacgcacc gcgatcccga tgtcgaggat cggcttcgcg
     4081 tcgagaccgg gcaccgcggt cgagccgatg tgctcgatcg cgacgacgga cgccggtgcc
     4141 gtcgcggcga ggacctcgtg gacgtccgcg aacgccaggg cccaggcatc cgacgccggc
     4201 gcgagatcca cgactccgcg tctcaacccc aggtccgcgt cgcggagggc atcggcgaga
     4261 cgggagccgg cggtctgcag catccctcca cgctaccggg agccggcggc accgccgctt
     4321 cagcgggacg cggcgtcgag cagggcgtcc tcgtcgtctg cgtcgcggtc gcgacggcga
     4381 cgcggcgggc gaggcgcacg ctcgccggcc tcctccttgg ccttgatcct ctcggtccgg
     4441 atcacgtagc cgatgaagat gaagcccatc accgcgaaca ggatccactg gatcgcgtac
     4501 gagaggtgcg ggccgggatc ctcggcgggc ttgtcgaagc cgccgagcgc cttcgacggc
     4561 gcgggcttct cggatgcgag cagtccgtag cctccggtga tgaggtccgg gttcacctgg
     4621 tcggcgatgg ccggaaggct gatcgtgggg acctgcccct ccggagcgct gcgacctgat
     4681 gccggcagct gctctccggg ctgcatccgc acgaccacgg tgacggtgcc ggccggggga
     4741 gcaggcgtgg atgcgggtga gctgtcctca cggggaggca cccagccgcg gtcgatcagc
     4801 agcacgcgac cgtcctcctg gcggaacggc accagcacct cgaacgcgct cgtgccgccg
     4861 tgcgggcggt tgcgcacgta cagctgctga tccgcgaggt actctccgcg caactggacc
     4921 ggatgccact cgcgccgcgc gtccagccgg ccctcggcat cgaccacgtc gtcgagggcc
     4981 acgggcttcg cgtcgtagtt gcgctccacc agtgcgatct cggtcgcctt ctggtcgttg
     5041 cggctgaact gccagttgga caggaacgcg cacgcgatcg cgaacaggat cgcgacgacc
     5101 aggtagccgc tccagcgcag cagccgctgc ctcactccgg cagcccttcg aagacctcca
     5161 ccgggaagtc ccgcgtacgc agataatcgc tgaggaagcc cacgtgctca tcgcacgcca
     5221 gccagatctt gcgacggtcc tcgctgtgga tccgcgggtt gcgccagacg atgtgatggg
     5281 tcgccgcggc gcggcagtcc gcgcgtgcgc actcgaactg cgccgtcacg atgcgtcgtc
     5341 ccgacgcggg gtgggacggc cgctctcgtg gatcgtgatg accttcggcg tctcctcggc
     5401 cgcggtctcc ggtgcatccg cctcgagttc catcacgggc gactcgaccg tcgtcggggt
     5461 gctgtcggcg ccggcgttcg cggcgaccac ggcgatgtag gggaggaaga tcgcccccgc
     5521 cgcgaacacg aacgtccacc acccgatcgg ctggacgacc gccatgagga tgaagcacgc
     5581 cgtgcggatg cccatggtga tcgcgtaatg gcgcacacga cgcttcgcct cgtcctgcgg
     5641 agcctccggg agcgaggtga cggagggggt gtgtcgggtg cttttcacgg tgactcaagc
     5701 ctacgccgcg cggggcgcga cggatgcccg cgtgaggctc cgaaaggcgg aagagcaggg
     5761 ccacggctcg ataggatggc cagcgttcat cccgacaggc aggagcccac catgagcacg
     5821 gaccgcgtcg tcctcgtcac cggcggaaac cgcggcatcg gccgcgccat cgccgagcgc
     5881 ttcgtccgcg acgggtaccg cgtcgcagtg accgcgcgca gcggcgaggg cccggagggc
     5941 acgctcaccg tgcgtgcgga tgtcacggat gccgcggcgg tcgatgccgc gttcaccgag
     6001 gtggagcaga agctcggccc ggtggaggtc gtggtcgcga acgccggcat cacgaaggac
     6061 accctgctca tgcgcatgag cgaggacgac ttcgacagcg tcgtctcgac gaacctcggc
     6121 ggctcgttcc gcgtcgtcaa gcgcgcctcg aagggcatgc tgcgcgcccg gttcggccgc
     6181 gtcatcctca tctccagcgt ggtcggcctg tacggctcgg ccgggcagac caactacgcc
     6241 gcgtcgaaga gcgcactggt cggcttcgcc cgctcgctga cccgcgagct cggaggccgt
     6301 ggcatcaccg cgaacgtcgt ggcgcccgga ttcatcgaga ccgacatgac ggcggagctg
     6361 cccgaggaga ctcagaagca gtacaaggca agcatccccg ccggccggtt cgccacgccc
     6421 gacgaggtcg cgagcgccgt cgtgtggctc gcgggcgacg actccggtta catctccggt
     6481 gccgtcatcc ccgtcgacgg cggcctgggc atggggcact gaccgccgcg ccgctcatcg
     6541 gctcaaggtc tcagcggtcg agcgccgcga cgatctcctg agcgaggacg tcgggcttgg
     6601 agaactgcgg ccagtggccg gttccgagct tgatgacctc ggcatcctcg atcgcgccga
     6661 actcctcggc gtacggtccc cactcgctga tcgccttctc gaaggcggcc tggtcgagac
     6721 cgcccatcag cagcgtgacg ggaaccgtgt accggcgagg gtcggtcagg ctgatcggat
     6781 cggtcggaac ccgtgccggg acgctcagcg ccagcggagc ggtgcgcgcc ctggtcgcgt
     6841 cgtcgaggtc gtgcacgtcc tcgtccggga agcgatccca gcccgggaac ggtacgacgc
     6901 cgtcgaccac ctcgaactcg ctgatgccgc ggccgggtgc cggcgggacg ctgtcgacga
     6961 acacgacgcg tgcgatccgc tcgggtctgg catccgtcgc gccccacacc acgttgccgc
     7021 cgccggaatg ccccacgagc gcgaccggtg cgtcgaagtc gtcgacccgg gaggcgacgg
     7081 cggcgaccca gtcggcgatg ccgatctcgg aggccggttc accgacgccg ggcatggtca
     7141 gcggatgcac gcgatggcct gccgcgcgca gcgcgggcag caccgcatcc cacgatgcgg
     7201 catcgagcca gagaccggga acaaggatga cgtccatggc ctccaccgta ggcaggggca
     7261 ccgacatcgt cgagtgcgaa cccggaaccg gcctgatccg gggatcaggg gagcagcggg
     7321 atgacctcgg acagatcctg agggccgacc accagcgcgg ccgcggcgcg caccgcgggc
     7381 ttggcgttga aggcgatccc gagacccgcc gcgctcatca tcagcagatc gttcgccccg
     7441 tcgccgatcg cgatggtggc gtgcggtgcg acgtccagct ccgccgccca ctgcttcagc
     7501 gagtcggcct tcgccttcgc atcgacgatc gcaccgtcga cctcgccgat gagctcaccg
     7561 tccgcggtcg cgaggcgatt ggcccgccag cggtccacgc ccagctcggg cgccacatgg
     7621 tcgaggatct cgtggaagcc gcccgagacg acgcccacga tgccgccccg ctcatgcacc
     7681 gccgcggtca ggtcgcgtac cccgggagtg ggctcgatgc gtgcgcgcac ccgttcgaag
     7741 gcatccgtcg ggacgccctt cagtgccgcc acccgggagc gcaggctcgt cgcgaagtcc
     7801 acctcgccgc gcatcgccgc ctccgtcgcc gcctgcactt cggcgcgacg tccggcctcg
     7861 tcggcgagca gttcgatcac ctcgttgcgg atgagggtgg aatcggcatc gaggacgacg
     7921 aggaagcgcg cggtggtcac cgctcgagcg tatcggccgg ccgaggtcat cgacgcccgg
     7981 atgtcggttc agcggcatcc gcggatcagc gcgcgatgcg gaggcccttc ccgaccacgg
     8041 tgataccgga gtccgtgatg gtgaagccgc gctccagatc gcgctcgcga tccacgccga
     8101 ccgtcgcgcc gtcctccagc acgacgttct tgtcgagcac ggctctgtgc acgcgggcgc
     8161 cggctccgac gagcacgccg tccagcagca cggagtcggt gatcgtcgat ccgccggatg
     8221 ccaggctgcc cacgcccacc acgctgcgct ccaggtgcgt gcccgagagc acggagcccg
     8281 ccgacacgat ggagtcgatc gtgttgccga tgcgtcccac cgcgtcgcgc acgaacttcg
     8341 ccggcggcgt gttcacggtc tgggtccgga tgggccaggc cgtgttgtac aggttgaaga
     8401 tcggcagggt ggagatgaga tccatgtgcg cgtcgaagta ggagtcgatc gttcccacgt
     8461 cccgccagta cgagcggtcg cgcggcgagg agcccgggac atcgttctgc ttcatgtcgt
     8521 agtagccggc ctcgccgcgt tcgacgaagt agggcacgat gtcgccgccc atgtcgtgcc
     8581 cggagctggc ggactcgccg tcggcctcca ccgccgcgat gagcgcgtcc gtgtcgaaga
     8641 cgtagttgcc catcgacacc agcacctcgt tgggcgagtc ggagaggccg gtcgggtccg
     8701 tcggcttctc caggaactcg cggatgcgac ccgtctcggg gtcggcgtcg atcacgccga
     8761 actgcgacgc cagagccagc ggctggcgga tgcctgcgac cgtggccgag gcgccggacg
     8821 cgatgtgcgc gtccaccatc tgctggaagt ccatccggta cacgtggtcg gcaccgatga
     8881 cgacgacgat gtccggcttc tcgtccagga tgaggttcag gctctgcagg atggcatccg
     8941 cagacccgga gaaccagcgc ttgccgaggc gctgctgggc gggcaccgac gtgacgtacg
     9001 aatcgagcag ggcggacatc cgccagttct gcgacacatg ccggtcgagg ctgtgggact
     9061 tgtactgcgt cagcacgacg acctgccgca gaccggagtt gatcaggttc gatatggcga
     9121 agtcgatgag ccgatactgc cctccgaacg gcaccgccgg tttggcccga tccgccgtca
     9181 gtggcatcag acgcttgccc tcgccgccgg cgaggatgat cccgaaaacc ttcttgggtg
     9241 ccgacatggg accaccatag agcccgaagc ggcggatgta ctaccgtgca gtcatgcgag
     9301 tcgacatcat caccaaggaa tatccgccgg agatctacgg cggtgccggt gtgcacgtcg
     9361 cagagctcgt cgccgcgctg cgcagcagca tcgacgtcca ggtccgcgcg ttcggatccg
     9421 accgtgatga acccggcaca catgcgtatc gcacccccgt ggagctcgcc ggcgccaacg
     9481 ctgccgtgca gacgctcggc acggatctcg cgatggtcgg ggacgtcgcc ggggcggacg
     9541 tcgtgcacag ccacacctgg tacgcgaact tcgccggcca cctcgcctcg cagctgcacg
     9601 gcatccctca cgtcctcacc gcgcacagcc tcgagccgct gcgtccctgg aaggccgagc
     9661 aactcggcgg cgggtacgcg gtctcgagcg gcatcgagaa gctcgcctac gagaacgccg
     9721 ccgcgatcgt ggcggtcagt gcaggcatgc gcgccgacat cctgcgcagc tatccccagg
     9781 tcgacccggc gcgcgtccgg gtgatccaca acggcatcga cgtggagcgc tggcggccga
     9841 tcgagaaccc ggagttcctg gcgagcatcg gcatggaccc tgcccgcccg tccgtcgtct
     9901 tcgtgggacg catcacgcgg cagaagggcc tgccctacct gttgcgggcc gcgcagctgc
     9961 tgccgcccga ggtgcagctg gtgctgtgcg cgggcgcgcc ggacactccg gagatcatgg
    10021 ccgaggtgca ggagctggtg cggctgctgc agcagaccag gcagggcgtg atctggatcg
    10081 agcggatgct gccgcgtgac gaactgtccg cgatcctgac cgcggccacg acgttcgtgt
    10141 gcccgtccgt gtacgagccg ctgggcatcg tcaacctcga ggccatggcg tgcggagcgg
    10201 ccgtggtcgg gacgcgcacc ggcggcatcc cggaggtggt cgcggacggc gtcacgggca
    10261 ggatcgtgcc gatcgagcag ctgcaggacg gcacaggcac gcccgtggac cccgagcagt
    10321 tcgtctccga cctcgccgcc gcgctgacgg aggtcaccgg tgatcccgaa cgtgccgcgg
    10381 agtatggcgc ggccgggcgc gagcgcgctc gaaccgagtt cagctggggc gcgatcgccg
    10441 acaccacccg cgcgctgtac gaggagctga ccgcctgagc cgataggctg gacgtcatgt
    10501 cgagcgccct ggatttcacc gatgtcgtcg tgcgccgtga cggtcgcgac atcgtcgatc
    10561 acgtcacctg gcaggtctcc gaggatcagc gctgggtggt gctcggcccg aacggggcgg
    10621 gcaagacgac cctgctgcag ctcgccgaca cgctgatgca cccgacgtcc ggaacggtga
    10681 cgatcctggg ggagaccctg ggccgcacgg acgtgttcga gatccgtcct cggattggct
    10741 tcgcgtcgac ggcgatggcc cggcgcattc ctcgcgacga gaccgtgctg aacaccgtta
    10801 tgacggcggc gtactcggtg atgggtcgct ggaacgagga ctacgagggc atcgacgagc
    10861 ggcgtgcccg ccgcgtgctg gccgactgga agctcgatca cctctccgag cgcctgttcg
    10921 gcacgctcag cgacggggag cagaagcgcg tccagatcgc ccgcgcggtg atgaccgacc
    10981 ccgagctgct gctgctcgac gagccgacgg cgagcctcga cctcggctcc cgtgaggagc
    11041 tgctccagct gctgagcggg tacgccgcat ctccgaccac tccggccatg atcatggtga
    11101 cccaccacgt cgaggagatc ccggtgggct tcacacacgt catgctgctg cgtgagggcg
    11161 gcatcgtcgc acagggcccg atcgccgaga cgctgacggc ggaggcgttg tccgaggcgt
    11221 tcggcatgcc gatcacactg agcgccgagg gcggccgcta cgcggctcgc gccgtcagcg
    11281 cctgagcacg gccgcggcga cccgtcgagg agtgctaaag tagacccttg gtgcaatcgc
    11341 accgcagact tccctcgctc ctggcagaat ccagggcacc acgtaaggaa tcccatgaag
    11401 actgacatcc accccgacta ccaggccgtc gttttccgcg acctcggctc gggcgagacc
    11461 ttcctgaccc gttcgacggt caccagcgac aagacgatcg agctcgacgg cgtcgagtac
    11521 cccgtcgtcg acgtcgagat ctcctccgcg tcgcacccgt tctacacggg caagcagcgc
    11581 atcatggact cggccggtcg cgtcgagaag ttcaaccagc gcttcaagaa cttcggcggt
    11641 tccagccaga agtaagccgg attcccgaag gccccgctcc ggcggggcct tcgtcgtttc
    11701 cggactcaga cgtcgatccg gatgcgggca ccgtcgatcg cccacggagg atcgccggcc
    11761 accggcgcag gagctgtgcg cttggtcatc cgatcgagtt cgacacctgc ctcgtgggct
    11821 gtcggcgcga agacggcctc gaggccgctc gcatagccgc ccgggactcc gcctccactg
    11881 acgaggtact tctgctcaag gggcttggac gagtagagga tcaccaggcc gatgctgccg
    11941 agcagaaccg gcagcgcgat caggagcacg atgatgctcc acccgaggat cgtggcgact
    12001 ggtgaggtca tgtcctcacg ctaagcgctg cacggaggcc gcgcatccgc ctcagggctg
    12061 atctcgagcc tcagccccgg acgggccagc tgccgtccag cgcctccgcc gggtcgaggc
    12121 gcccgacctt gatgaagtac tccgtcaggc tctcggcctg atcacgcgcc cagccgatct
    12181 gctgggtgtg gagctcggcc gcggtgccgg ggagggcgaa ctccttcgcg agggccagcg
    12241 cgacgcgccc ggccgccacg gcgtccgcgg aggcctcgtg cgcatcgtcg agcggaacgg
    12301 cgtagtgctc ggccaccacg ctcagggtgc gcttgccggg acggtagcgg tcgtacgcct
    12361 tgtcgatcac gagcgggtcg atgaccggct gcggatcgat cagcaccggg acatcgtggc
    12421 gggccgcctc gtgcgcgagc agggagaagt cgtaggaggc gttgtatgcc acgatcggca
    12481 taccctgggc gaagagcaca cggagggcgt tgctgatctc cgcgacgacc tcgcccgccg
    12541 gccgcccgtg cgtcctggca tgctcggtgc tgatgccgtg cacggccgcg gcgccgtcgg
    12601 ggatggggat gcccgggtcg gcgagccagt ccctcgccgc gatctccctt ccgtgccggt
    12661 cgagcacacc cacgtgcgcc gtgacgatgc gatcggtggt gacgtcgaca ccggtcgtct
    12721 cgaggtcgaa cacgcccact cgggtcagcc agttcgggag cgtcgtcgag gtcatggcct
    12781 caccgtagga cggggcgccg acatccgtcc ggtggcccgc cgggatgccc ctacactgtg
    12841 caggtgacct ggactgtgtg gctctcgctg ctgctggcct gcgtggtgat cagcttcacg
    12901 ccgggggcag gcgcgatcaa caccatgagc aacgcgctca cgcagggctg gcgacgctcg
    12961 ctgtggggag ttctcggcca gcaggcggcc ctcgtcgtgc acgtggtcat cgtcgccgcg
    13021 ggtgtgggcc tgctggtgtc gcggtcgcag ctgctgttcg acatcatccg ctatgcgggc
    13081 gccgcgtacc tggtctatct cggcatccgc ctgatcctcg cgaagcctca ggtggagacg
    13141 atggcgacgg catcgcggtc ccgtgagcac gcctggtcga tcttccggcg cggtttctgg
    13201 gtgaacctcc tcaaccccaa ggcgatcgtg ttcttcctcg cgttcgtgcc gcagttcatc
    13261 cggctcgatc aggacccgct gccgcagtac ctgatcctga tcggcacggt gatcgtcgtg
    13321 gacgtgctcg tgatgtggtt cttcttcgcc gccgccgcga agccgttccg ccgcttcacg
    13381 agggatgccc gcgggcagcg gatcctgaac agcatcttcg gctcgctgtt catcgccgtc
    13441 gccggtctgc tgctgttcgt gcactgacgc atcacggtct tcctgcacag acaccgttct
    13501 cgggaagttc tccacgggcg tcgggaatcg gcctccgggc gtcggatgtc gcgatgcacc
    13561 ctgttctcac ccgggttcga cccggcaaca caggagcagg acatgaccat caggaacgac
    13621 accatcgtga tcgccgggaa catcggcaac gatcccacga agaacgagac ccgcaatggc
    13681 aaggctgtga tcaacttccg ggtcggcacg tcgcacaact acatcgatca gcgcaccggc
    13741 gaatgggtcg agagcccgac cagctggtac aacgtctccg cgttcggcaa catcgccgag
    13801 cacgcgaagg cctcgttgca ccgcggcgat cccgtcatcg tggtcgggcg tctgcggatc
    13861 cgggactggg aggccggcga tgggaagaag ggccgggagg cggagatcac cgccgacgcc
    13921 atcgggcacg acctgaactg ggggaccagc gccttcgtgc gccgccagcg cgcgactgcg
    13981 gagcctgtgc aggcaccgca gccctccgac gccgacgctc catcggacga tgcgcccagc
    14041 gcggacgagg gcgatgcgtg gcgcatcgcg gggctggagg tcctcgccga ggacgaagcc
    14101 tccaaggagc ccgccttcac gtgagggccg agcgcgtcga cggatggccc tcggtgctgc
    14161 gcctagactt ctgagcgtgc ttcgagggtc ttccgtctcc gctccccgca tccgggcggt
    14221 ggcgacgatc gccggagcga cgctcatcgc ggcggctctc gcgtcctgct cggctccggc
    14281 tgagaagccg gatcctgcgg cctcctccgc agtcccctcg ccgtcgacat ctgcggcaga
    14341 ggcgccggtg ctcgtcccgg acgggacggc gacggacaac ctggcgctgt tcaccgcggt
    14401 gaccgcgcag gtctggggtt ccgatgaccg cgcccggggc cgtgcgtacg tcgacgcgct
    14461 cgtcgccgcc ggcttcccga aggatcggat gcaggtcacc ggcgatcaga ccacggtcgg
    14521 gcgcgcggcg gagagcctgc agttctcggt ggcctggggc gacgaggagt gcctgatcgg
    14581 acaggtggga acgtcgacag gcgatccggt gacggcagtc atgccgcagc tcgccagcgg
    14641 ccggtgcctc gtggggcgaa cgcggccgat cgactggtga cggccgctga caggtcggtc
    14701 gcgaagcgca ccggtgaaga cgggtaggct ggtgtgtcgg ctccccggag ccgttccgcg
    14761 tgcccgctga ctgaaggaga gctgcttcgt ggccgagtac atctattcca tggtgcgagc
    14821 ccgcaaggcg gtgggcgaca agctcatcct cgacgacgtc accatggcgt tcctccccgg
    14881 agcgaagatc ggcatggtcg gccccaacgg cgccggaaag tcgaccatcc tgaagatcat
    14941 ggcgggtctc gacaccccgt cgaacggcga ggccaagctg acgccgggct tctcggtggg
    15001 catcctcatg caggagccgg agctcgacga gtccaagacc gtgctggaga acatccagga
    15061 cggcgtggcg atcaagccca agctcgaccg cttcaacgag atctcggccc tgatggccga
    15121 ccccgacgcc gacttcgatg cgctgctcgc cgagatgggc gtgctgcagg aggagatcga
    15181 cgctgccgat ggctgggacc tcgactcgca gctcgagcag gccatggacg cgctgcggac
    15241 gccgtcggcg gatgccgaga tcgcaccgct ctccggtgga gagaagcgcc gcgtcgcact
    15301 ggccaagctg ctgctgcaga agcccgacct gcttcttctc gacgagccca ccaaccacct
    15361 cgacgccgag agcgtgctgt ggctcgagca gcacctgaag gactacaagg gcgccgtgat
    15421 cgccgtcacg cacgaccggt acttcctcga ccacgtcgcc gagtggatcg ccgaagtcga
    15481 ccgcggtcgc ctgctcggct acgagggcaa ctactccacc tacctggagc agaaggccga
    15541 gcgcctcgac atccagggca agaaggacgc caagctcgcc aagcggctga aggacgagct
    15601 cgagtgggtg cgctccagcg ccaagggccg gcagacgaag tccaaggcgc gtctggcgcg
    15661 ctacgaggag atggcggccg aggccgagcg caccaggaag ctcgacttcg aggagatcca
    15721 gatccccgcg ggtccgcgcc tcggcaacat cgtcatcgag gcgaagaacc tgcagaaggg
    15781 cttcggcgac cggatgctga tcgacgggct gagcttcagc ctgccgccga acggcatcgt
    15841 cggcgtgatc ggccccaacg gcgtcggaaa gacgacgctg ttcaagacca tcgtcgggct
    15901 ggagccgctc gacggcggcg acctgaaggt cggcgagacc gtcaagatca gctacgtcga
    15961 ccagacgcgc gccaacatcg accccaacaa gacgctgtgg gaggtcgtct ccgacggcct
    16021 cgacttcatc accgtcggca agatcgagat cccctcgcgc gcctacgtgt cgaagttcgg
    16081 tttcaagggc ccggaccagc agaagagggc cggagtgctc tccggtggtg agcgcaaccg
    16141 cctcaacctg gctctgacgc tgaaggaggg cggtaacctg ctgctcctcg acgagcccac
    16201 caacgacctc gacgtggaga cgctgagctc gctggagaac gccctgctgg agttccccgg
    16261 ctgcgccgtg gtcatcacgc acgaccggtg gttcctggac cgcatcgcca cgcacatcct
    16321 cgcctacgag ggcaccgacg agaacccgtc gcagtggcac tggttcgagg gcaacttcga
    16381 ggcgtacgag aagaacaaga tcgatcggat gggcctggag gcggcgcgcc cgcaccgtac
    16441 cacgcaccgc aagctccacc gtgactgatc cctcgcccca ctcgggacag cgcttcgcgc
    16501 tgccccgagc ctcgggggcg cggggccctc tcgccatcaa cgatccgaga agcgcatgac
    16561 cgcccgcgag aacgcgcaag tgcccgtcga acgcattctg gacgacgggc gtctgcacat
    16621 ccccatccag ttgcgctggg gcgatctcga cgcgttcaat cacgtcaaca acacggcgat
    16681 gctgaagctg ctggaggagg cgcgcgtgcg cgccttctgg aagccggcgc ccggcgaggt
    16741 ggcccctccc acggcgatcc tggactccgg gctgtcctac ggcctgctca cgctcatcgc
    16801 gcgtcaagag atcgagtacc tggcgccggt gccgtaccag cggcatccgc tggacatcca
    16861 gatgtggttc ggcaagctcg gcgggtccag catcgaggtc tgctacgagg tgcgaccgga
    16921 tgccgcagcc gagtcggacg tgctgtatgc gcgcgccacg accgtgatcg tgctcgtgga
    16981 cgccgagtcg ggtcgacccg tgcggctcac gcccgagatg cgctcggcct gggagccgct
    17041 ggtcggagtg ccgctggagt accggcggca ctgagagcgc gctcccgaaa gggcgctgcc
    17101 cggagtcaga gctccggaac ccgcaccatg atctcctgcg cgacggtggc catcagcacg
    17161 ccgtcgcggg tgtagacccg gccctgcgcg aggccgcggc cgccgcgggc gttgggggag
    17221 ttctgcacgt acagcatcca ctcgtcgact cggccgaagc ggtgccacca catggcgtgg
    17281 tcgaggctcg cgaccttcag ccccggcgtc tgccaggtga tgccgtggcg acgcaggatg
    17341 gactcctgga tcgtcatgtc gctgagatac gcgagcgctg cccggtgcac ccgggggtcg
    17401 tcgggcatcg gcgcccgcat ccgcatccag accgcctgct gcggcacatg ctcaccggcc
    17461 acttcgagga acagcggacc ggtcacgtgg cggatgtcgg ccgggcgatc ggccagcagg
    17521 cgtcctgtga tcggatgcac ggcgccgatg agttcgtcgt ccgaaggcag cgactccggt
    17581 tcggggatgt cctcggggaa cggctcggca tggtcgacgc cgggatcctc gtcctggaac
    17641 gacgagatca tcgagaagat cggcacaccg ccctggaagg cctgcacgcg gcgcgtggag
    17701 aaggacctgc cgtcgtggat ccggtcgacg gcgaacgtga tcccctgggt ggcgtcgccg
    17761 gggcgcagga agtagccgtg catggagtgc acgatccggt cctccggaag cgtccgctca
    17821 gcggcgacca gggactgcga gagcacctga cctccgtata cgcggccggt cggcatcgcg
    17881 tgcgagacgc cggtgaagat gtcctcggtg gtgcgggcct cggaggcgtc gagatcgagc
    17941 acctggagga gctggctgac ggaccgacgg tcgttctcct gagtgctcac gcgactagtt
    18001 tagaaggagt gaccagacct ctcgttctcg ccgacgccga caccgcccgc gacgtgctga
    18061 ccttctccgg ccgcgcctcg aaagcagggg ccgacggcgt ccgcctgcag gcgacgggcg
    18121 gcgtgctcgc ggtcacctcc gccgcgctcg ccccgcaggg gctgttcgat cagacgccga
    18181 cgatcctggc gatgcggatc gtgcgcgccg atcccgagct gagctgcgac ctggtcgtgg
    18241 actcactgaa ggaggggcgg aatcccgatg agctggcact gccggacacg gcgctgtccc
    18301 cggcctgggc gggcatcgcc ccgccgcggg gcggatggga gcatgtggcg gatctcccgg
    18361 ccgggctgat cgccgaacgc gcgctgtggg ggatctccgc tgtcgcgcac ggaagtcccg
    18421 acggtgcggg ggaggacgcc gtgcgggccc tccgcgcccg catctggggt agatcgg
//
LOCUS       DATMSX010000006         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_75889,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000006 DATMSX010000000
VERSION     DATMSX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75889"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN041_00245"
     CDS             complement(<1..524)
                     /locus_tag="VN041_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWU27508.1"
                     /translation="MHRAAYTERMSSAFLTDRYELTMLAASLRDGTASRPSVFELFSR
                     RLSGGRRFGVVAGTGRLLSLLREFRFEEDELRYLRDNDVVDAAAVKYLEDYRFTGTIL
                     GYREGELYFPGSPILTVEGTFADAVVLETLALSVLNHDSAVATAASRMSIAAGERPLA
                     EMGSRRAAEHSAVAA"
     gene            585..>1769
                     /locus_tag="VN041_00250"
     CDS             585..>1769
                     /locus_tag="VN041_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU27509.1"
                     /translation="MSVPDDFERREIERLANAVLWPGFLGNEVPDWLADALRDGLAGV
                     VYFAQNLGGDTTALSAEIHRIAPHAMIGIDEEGGSVTRLETTTGSTIPGAAELGALDD
                     VEATRATGYEIGRRVAAIGADVVIAPVADVNTDPRNPVIGVRAFGSDTSLVSRHVAAA
                     VTGIQDAGVAACAKHYPGHGDTHTDSHHDLPRITLDQAEIERVHLPPFTAAVDAGVLS
                     IMTAHIAAPQWGDAPATLNPLVLGRLRAAGFDGVIVTDALDMAAIRESVGIGGGAVRA
                     LAAGADLLCIGNPTNPGDAMLADQDERDYREARDAVVAALRSGELSRERVEEAARRVA
                     VMAAAVRDRGPTAETPFDGDAIADRSVRVDGSFPAFEDAPTTVIDLRRASSLAVDSAA
                     SHV"
BASE COUNT          267 a          638 c          626 g          238 t
ORIGIN      
        1 gccgcgacag cggagtgctc ggcggcacga cgcgagccca tctcggcaag cgggcgctcg
       61 cccgcggcga tgctcatccg ggaggccgcc gtcgcgacgg cggagtcgtg gttgagcacg
      121 ctcagcgcca gggtctccag cacgacggcg tccgcgaagg tgccctccac ggtgaggatg
      181 ggggagccgg ggaagtacag ctcgccctcg cggtagccga ggatcgtgcc ggtgaaccgg
      241 tagtcctcca ggtacttcac cgcagcggcg tccaccacgt cgttgtcgcg cagataccgc
      301 agctcgtcct cttcgaagcg gaactcgcgc agcagcgaga gcagccgtcc cgtgcccgcg
      361 acgaccccga aacggcggcc gccggagagg cgccgggaga acagctcgaa gacggagggc
      421 cggctcgccg tgccgtcgcg gagcgaggcg gcgagcatcg tcagttcgta gcggtcggtg
      481 aggaacgcgg agctcatccg ctcagtgtat gccgcgcggt gcatcgcttc ctgtccggtg
      541 acggatgccg cgcgctgtgc cccgctccgg aatcggggat actgatgtcc gtgcccgatg
      601 acttcgaacg gcgtgagatc gaacgcctcg ccaatgccgt gctctggccg ggattcctcg
      661 gaaacgaggt gccggactgg ctcgcggatg ccctgcggga cggcctcgcc ggagtggtgt
      721 acttcgcgca gaacctcggc ggtgacacca cggccctcag cgccgagatc caccgcatcg
      781 ccccgcacgc gatgatcggc atcgacgagg agggcggcag cgtcacgcgg ctggagacga
      841 ccaccggatc cacgatcccc ggcgccgccg aactgggcgc gctggacgac gtggaggcga
      901 cgcgcgccac ggggtacgag atcgggcgcc gagtggccgc gatcggcgcc gatgtcgtga
      961 tcgcgcccgt cgccgacgtc aacaccgacc cgcgcaaccc ggtgatcggc gtgcgcgctt
     1021 tcgggtcgga cacctccctg gtctcgcgtc acgtggccgc ggccgtcacc ggcatccagg
     1081 atgcaggggt cgccgcctgc gccaagcact atccgggaca cggcgacacc cacaccgact
     1141 cgcaccacga cctgccccgg atcactctcg accaggccga gatcgaacgc gtgcacctgc
     1201 cgcccttcac cgccgcggtg gatgccggag tcctgtcgat catgaccgcc cacatcgccg
     1261 ccccgcagtg gggcgacgcg cctgccaccc tcaaccccct ggtgctcgga cgactgcgcg
     1321 ccgcgggttt cgacggcgtg atcgtgacgg atgcgctcga catggccgcg atccgggaga
     1381 gcgtcgggat cggcggcggg gccgtgcggg cgctcgccgc cggcgccgac ctgctctgca
     1441 tcggaaaccc cacgaaccct ggcgatgcga tgctcgccga ccaggacgaa cgcgactatc
     1501 gcgaggcgcg ggatgccgtg gtggccgccc tgcgatccgg cgagctgtct cgcgagcgcg
     1561 tcgaggaggc tgcacgacga gtggccgtga tggccgcagc tgttcgtgat cgcgggccga
     1621 ccgccgaaac gcccttcgac ggtgacgcga tcgccgaccg ctccgtgcgc gtcgacggat
     1681 cgttccccgc gttcgaggac gcgcccacga cggtgatcga cctgcgccgg gcgtccagcc
     1741 tcgccgtcga cagcgccgcc tcgcacgtc
//
LOCUS       DATMSX010000007         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19089,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000007 DATMSX010000000
VERSION     DATMSX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19089"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..158
                     /locus_tag="VN041_00255"
     CDS             15..158
                     /locus_tag="VN041_00255"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HWU27510.1"
                     /translation="MLGPTADRPIAMGYVATAYSEPGTPLTVTVDRGSLRARSEGEIP
                     EAG"
     gene            250..489
                     /locus_tag="VN041_00260"
     CDS             250..489
                     /locus_tag="VN041_00260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWU27511.1"
                     /translation="MDVVTASNDSPVESLSFEAARDELVRVVAELEQGSPTLEHSLAL
                     WERGEALAARCEEWLLGAKRRLEAARDAASAGEEA"
     gene            486..1127
                     /locus_tag="VN041_00265"
     CDS             486..1127
                     /locus_tag="VN041_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 family protein"
                     /protein_id="HWU27512.1"
                     /translation="MSRKPGIVAELGRPETPQETADRKAESSRVYRSSQTFRNLIAAL
                     IITVAVVAVIVFAVPRGEPTARPAIDVAKIAADVSSTSDRPVIVPELGEFWRVNAAGL
                     DGGATKVWSITLAPAGERERGFIKLAQAFDADVKWAPQVTGGMAPTGTEQIDGVTWDV
                     FDLDDRGTANVTYALGTQAGRDYVLLYGSRSADSTTKLAESLTSQLSDLKEAG"
     gene            1124..1795
                     /locus_tag="VN041_00270"
     CDS             1124..1795
                     /locus_tag="VN041_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWU27513.1"
                     /translation="MTTTLTPAEAWTKLIEGNRRFVAGISEHPNQDAHRRAEIAGGQN
                     PFATFLGCSDSRVAAETLFDVGLGDLFTTRNAGQMVTDSAVASIEFAVAMLDVPLLVV
                     LAHDSCGAVAAAVRGTAIDAEPIPPKIWKLIAPIVPAARRVLKESGGTTVEDIDPEEV
                     GREHLRGTILGLLESSEIISDAVAEGRLAIVGAKYRLTDGSAVPIAQVGLADSDIPSL
                     SKEQK"
     gene            1792..3186
                     /locus_tag="VN041_00275"
     CDS             1792..3186
                     /locus_tag="VN041_00275"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWU27514.1"
                     /translation="MTETEYRIEHDTMGEVRVPVNALYSAQTQRAVENFPISGKGLEP
                     AQIAALARIKKAAALANKDLGTLDGAIADAIAQAADEVATGAHDGEFPIDTYQTGSGT
                     SSNMNMNEVLATLASRILGSAVHPNDHVNASQSSNDVFPTSVHVAVTQALIDDLIPAL
                     DHLAVAFEAKADAWKSVVKSGRTHLMDATPVTLGQEFGGYARQMRLGIERVQAALPRV
                     AEVPLGGTAVGTGINTPLGFPQKVIELLAAETELPITEAKDHFEAQANRDGLVEASGA
                     LRTIAVSLTKINNDLRWMGSGPNTGLAEIHLPDLQPGSSIMPGKVNPVIPEAVLMVCA
                     RVIGNDATIAWGGASGAFELNVAIPVMGTALLESIRLLANATRVLADKTVDGLQANVE
                     RAAALAGMSPSTVTPLNKIIGYEAAAKIAKHAVAKGITVRDAVIDLGYVERGEITLEV
                     LDQKLDLLSMTHAG"
     gene            3355..4209
                     /locus_tag="VN041_00280"
     CDS             3355..4209
                     /locus_tag="VN041_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWU27515.1"
                     /translation="MPDADVMIDPQILITRLGGVARGRQLQEFGVSRMRLSGAVREGH
                     VTRIRDGLFATDAAHPEELAAARHGGALTCSSALRRHGVWVLAEPGRPHVWMGRRGRA
                     YEHDGCHCISHYFRGASAFGVVDVATALIHLHRCEGEEAFFASLESALRLRLISDASR
                     SRIRAALPAHARWLVDLAHEDADSGLESLLRLRMHQLGIRLETQVRIDGVGRVDFVVG
                     GRLIIEADGKDNHAAADRRHKDLVRDAAASALGYETLHFDYAQIIYDWPTVQAAILAA
                     LARLSHRW"
     gene            complement(4405..5712)
                     /locus_tag="VN041_00285"
     CDS             complement(4405..5712)
                     /locus_tag="VN041_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWU27516.1"
                     /translation="MSHDDTALRAYVLDTSVLLSDPQALFRFAEHSIVLPVVVISELE
                     AKRNDPELGYFARRALRHLDELRVEHGRLDFPVEVGEGGTLRVELGTADLSILPAGIR
                     LSDNDSRILATAAQLAQDGQAVTIVSKDLPMRVKAASLGLQAEEYLAEQAIDSGWTGI
                     APLDLSGDEMSDLYESEVGLSEQVRGVPVNTGLIIHSERGSALGRVTGDGEYRLVRGD
                     REVFGLHGRSAEQRIAIDLLLDPEVGIVSLGGRAGTGKSALALCAGLEAVLERQQQKK
                     IIVFRPLFAVGGQELGYLPGDREEKMNPWGQAVYDTLGSVVSGNVLDEVVERGMLEVL
                     PLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRMGQNSRVILTHDVGQRDNLRVGRH
                     DGIASVIETLKGHDLFGHVTLTRSERSAIAALVTELLEGGELS"
BASE COUNT          958 a         2194 c         2002 g          895 t
ORIGIN      
        1 ttgtcacgag cggcctgctc ggcccgacgg ccgaccgccc gatcgccatg ggctacgtgg
       61 ccaccgcgta ttccgaaccc ggcacgccgt tgacggtgac cgtcgatcgc ggctccctac
      121 gcgcccgctc cgagggcgag atccccgagg caggctgacg cccgcgcccc atcggtggtg
      181 cgctgggctg tgtccatcca ccccgggctg cgccctgccc ggacagggcg tccgtgacac
      241 gccggtagga tggatgtcgt gactgcgtcc aacgactccc ccgtcgaatc gctgtcgttc
      301 gaggccgcgc gcgacgaact cgtgcgcgtc gtcgccgaac tcgaacaggg atcccccact
      361 ctcgagcact ccctcgccct ctgggagcgg ggtgaggcgc tggccgcccg ctgcgaggag
      421 tggctgctgg gcgcgaagcg ccgtctcgag gccgcccgcg acgcggcatc cgccggagaa
      481 gaggcatgag ccgcaagccg gggatcgtcg ccgagctcgg tcgcccggag actccgcagg
      541 agacggccga ccgcaaggcg gagtcgagcc gcgtctaccg gtccagccag accttccgca
      601 acctcatcgc cgcgctgatc atcaccgtcg ccgtcgtcgc ggtgatcgtg ttcgccgtcc
      661 cccgcggcga gcccaccgcg cgcccggcca tcgatgtcgc caagatcgcg gccgacgtct
      721 catcgacctc ggatcgcccg gtgatcgtgc ccgagctggg cgagttctgg cgagtgaacg
      781 ccgcagggct ggatggcggc gccaccaagg tgtggagcat caccctcgcc cccgcgggcg
      841 agcgcgagcg cggcttcatc aagctcgcgc aggcgttcga cgccgacgtg aagtgggcac
      901 cgcaggtcac cggggggatg gccccgaccg gcactgagca gatcgacggc gtgacctggg
      961 acgtgttcga tctggacgat cgcggcacgg cgaacgtcac ctacgcgctc ggcacccagg
     1021 ccggccgcga ctacgtgctg ctctacggct cccgctccgc ggactccacc acgaagcttg
     1081 ctgaatcact cacctcgcag ttgagcgacc tgaaggaggc cggatgacca cgacactcac
     1141 ccccgctgag gcctggacga agctgatcga gggcaacagg cgattcgtcg ccggcatctc
     1201 cgagcacccc aaccaggacg cgcaccgccg ggccgagatc gcgggcggac agaacccgtt
     1261 cgccaccttc ctgggctgct ccgactcgcg cgtcgccgcg gagaccctgt tcgacgtcgg
     1321 cctgggcgat ctgttcacca cccgcaacgc cggccagatg gtgacggact cggccgtggc
     1381 gagcatcgag ttcgccgtcg cgatgctcga cgtcccgctg ctggtcgtgc tggctcacga
     1441 ctcctgcggc gcggtcgccg ccgccgtgcg cggcacggcg atcgatgcgg aaccgatccc
     1501 gccgaagatc tggaagctca tcgccccgat cgtccccgcc gcgcgtcgcg tgctgaagga
     1561 gagcggcggc accaccgtcg aggacatcga ccccgaagag gtcgggcgcg agcacctgcg
     1621 cggcaccatc ctcggcctgc tggagtcgtc cgagatcatc agcgacgccg tcgccgaggg
     1681 tcgtctggcc atcgtgggcg ccaagtaccg cctgaccgac ggcagcgccg tccccatcgc
     1741 acaggtcggc ctcgccgact ccgacatccc ctcactctcg aaggagcaga agtgactgag
     1801 accgaatacc gcatcgagca cgacaccatg ggtgaagtgc gtgtccccgt gaacgcgctg
     1861 tacagcgcgc agacgcagcg cgccgtcgag aacttcccga tctcgggcaa gggcctcgag
     1921 cccgcgcaga tcgccgcgct ggctcgcatc aagaaggccg ccgcgctcgc gaacaaggac
     1981 ctcggcaccc tcgacggcgc catcgccgac gcgatcgccc aggccgccga cgaggtggcc
     2041 accggtgcgc acgacggcga gttcccgatc gacacgtacc agaccggctc cggcacctcg
     2101 tcgaacatga acatgaacga ggtgctcgcc acgctcgcct cgcgcatcct cggctccgcg
     2161 gtgcacccca acgaccacgt caacgcgtcg cagtcctcga acgacgtgtt cccgacctcg
     2221 gtgcacgtcg ccgtcacgca ggcgctgatc gacgacctca tcccggctct cgaccacctc
     2281 gccgtcgcgt tcgaggcgaa ggcggatgcc tggaagagcg tcgtcaagtc gggccgcacc
     2341 cacctgatgg acgccacccc ggtcaccctc ggccaggagt tcggcggcta cgcccgccag
     2401 atgcgcctcg gcatcgagcg cgtccaggcg gccctccctc gcgtcgccga ggtcccgctc
     2461 ggcggcaccg ccgtcggcac cggcatcaac accccgctgg gcttcccgca gaaggtcatc
     2521 gagctgctcg ccgccgagac cgagctgccg atcaccgagg ccaaggacca cttcgaggcc
     2581 caggccaacc gcgacggcct ggtcgaggca tccggtgccc tgcgcaccat cgccgtgtcg
     2641 ctgaccaaga tcaacaacga cctgcgctgg atgggctcgg gccccaacac cggcctcgcc
     2701 gagatccacc tgcccgacct gcagccgggt tcgtcgatca tgccgggcaa ggtcaacccg
     2761 gtcatccccg aggccgtgct gatggtgtgc gcccgcgtca tcggcaacga cgccacgatc
     2821 gcgtggggcg gcgcatccgg cgccttcgag ctgaacgtcg ccatccccgt gatgggcacc
     2881 gcgctgctcg agtcgatccg tctgctggcc aacgccaccc gggtgctggc cgacaagacc
     2941 gtcgacggcc tgcaggccaa cgtcgagcgc gccgcggccc tggccggcat gagcccgtcg
     3001 accgtgaccc cgctcaacaa gatcatcggc tacgaggcgg cggcgaagat cgccaagcac
     3061 gccgtcgcca agggcatcac ggtccgcgac gccgtgatcg acctgggcta cgtcgagcgc
     3121 ggcgagatca ccctcgaggt gctcgaccag aagctcgatc tgctgagcat gacccacgcg
     3181 ggctgacgcc cgcgtcggtt ccacttcggg ggccgacacg ccggatttcg gacgtttcca
     3241 gcgagaacgt tcgaaatttg gcgtgtcggc ccccgttgtg tctccttgca caatcacgac
     3301 cgcggggcga gttgtgcaca ggtgccgggt tcgccgcgtc gctgggatga acggatgccc
     3361 gatgctgacg tcatgatcga tccgcagatc ctcatcacgc ggctcggagg agtcgccaga
     3421 ggcaggcagc tccaggagtt cggcgtcagt cggatgcggc tgagcggggc tgtgcgcgag
     3481 ggtcacgtca cgcgcatccg tgacggactc ttcgcgacgg acgccgccca tcctgaagaa
     3541 ctcgcggccg cacgccacgg aggcgccctg acgtgctctt cagcgctgcg tcgtcacgga
     3601 gtgtgggtac tggcggagcc cgggcgaccg cacgtgtgga tgggtcgtcg tggacgcgcg
     3661 tatgagcatg acggatgcca ctgcatcagc cactacttcc gaggcgcatc cgccttcggg
     3721 gtggtcgacg tcgcgaccgc gctgatccat ctgcaccgct gcgagggaga ggaggcgttc
     3781 ttcgcctcac tcgaatccgc actgaggctg cgcctgatct cggatgcgtc acgcagccgc
     3841 atccgcgccg cgcttccggc ccacgcacgt tggctggtcg atcttgccca cgaagacgcc
     3901 gatagcgggc tggagtcgct acttcgactg cggatgcatc agctggggat ccgtcttgag
     3961 acacaggtgc gcatcgacgg cgtcgggcgg gtggacttcg tggtcggcgg acgcctgatc
     4021 atcgaggcgg atggcaaaga caaccacgcc gcggcggacc ggcgtcacaa agacctggtg
     4081 cgggacgccg cggcatccgc tctcggctat gagacgctgc acttcgacta cgcccagatc
     4141 atctacgact ggccgaccgt gcaggccgca atcctcgccg ccctcgcccg cctctcgcac
     4201 cgttggtaac tcgcacctca acgtcgcgcc tgacacacaa tgggggccga cacgccagat
     4261 ttcggacgtt ctcagcgaaa acgtccgaaa tgtggcgtgt cggcccccga agtgcgtacg
     4321 acgtcgtgca tccgttcccg ggtcgtgcag gagtgccccg ctgcggcgga tcgggacgcg
     4381 cccgatccgc cgaggcggat gccgtcagct gagctccccg ccctccagca gctccgtgac
     4441 cagcgccgcg atcgcggagc gctccgaccg ggtgagggtg acgtgcccga acagatcgtg
     4501 ccccttgagc gtctcgatca cgctcgcgat gccatcgtgc cggccgaccc gcaggttgtc
     4561 gcgctgcccc acgtcgtgcg tgaggatgac gcgcgagttc tgccccatcc ggctgagcac
     4621 tgtcagcagc acgttgcgct ccagcgactg cgcctcgtcg acgatcacga aggcgtcgtg
     4681 cagcgagcgc ccccggatgt gcgtgagcgg cagcacctcc agcatcccgc gctccaccac
     4741 ctcgtcgaga acgttgcccg agacgacgga gccgagggtg tcgtacaccg cctggcccca
     4801 cgggttcatc ttctcctcgc gatcgccggg gaggtagccg agctcctgcc cgccgaccgc
     4861 gaacagaggc cggaacacga tgatcttctt ctgctgctgc cgttccagca cggcctccag
     4921 gcccgcgcac agcgccagtg ccgacttgcc ggtaccggct cgcccgccga gcgagacgat
     4981 cccgacctcg gggtcgagca gcaggtcgat cgcgatgcgc tgctcggccg accgtccgtg
     5041 cagtccgaac acctcgcggt caccccgcac caggcggtac tccccgtcgc cggtgacccg
     5101 gccgagagcc gagccgcgct cggagtggat gatcagcccc gtgttgaccg gaaccccgcg
     5161 cacctgctcg ctcaggccga cctcgctctc gtagaggtcg ctcatctcgt cgccggagag
     5221 gtcgagcggc gcgatgccgg tccaacccga atcgatggcc tgctcggcga ggtactcctc
     5281 ggcctgcaga ccgagggatg cagccttgac gcgcatcggc aggtccttgg agacgatcgt
     5341 gacggcctga ccgtcctgcg cgagctgcgc ggccgtggcg aggatgcggc tgtcgttgtc
     5401 gctcagccgg atgccggcgg gcaggatcga caggtcggcg gtgccgagct cgacgcgcag
     5461 cgtgccgccc tcaccgactt cgaccgggaa gtccagccgc ccgtgctcga cccggagctc
     5521 gtcgagatgc cgcagagcgc gtcgcgcgaa gtagccgagc tcggggtcgt tgcgcttcgc
     5581 ctcgagctcc gagatcacca cgaccggcag gacgatcgag tgctccgcga agcggaacag
     5641 ggcctgcggg tcgctgagca gcaccgaggt gtcgagcaca tatgcgcgaa gagccgtgtc
     5701 atcgtgtgac acggctccgg ttgcgacggc cttgcgggtg gactgctgct gcgctgaacg
     5761 tgtggtcacg atccactccc ctatccggga tgatcccggc tctgcagcga gtcgaccagg
     5821 ggccacgagt cgcgatcctg gaggccgact cgatcgggca cggtgtccga tgcctagaag
     5881 ctacgacccg ctcggtcgta cgccgcgaac gacgcgcggg cgtgtcgtat taagaggtga
     5941 tgaacgtccg gtttccggat gccgtgagcg cgtcgtccag catccgcagc gtctccgcac
     6001 cggtgccggc gtgcgcggcg acgcgcacgg ccccgccgcg gacggtgac
//
LOCUS       DATMSX010000008         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19099,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000008 DATMSX010000000
VERSION     DATMSX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19099"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="VN041_00290"
     CDS             complement(<1..1993)
                     /locus_tag="VN041_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HWU27517.1"
                     /translation="MSTPVSASEASVNDLAELADDAVALVKKWLVDSREVPVDAAGQR
                     LAGVLRDPNGLDFTVGFVDGVVRPEDLKVAAAKLKELVPLTPAFLPGIMKGAIGLGGA
                     TATMLPGIVVPSARAVLRQMVRHLIVDARDEKLGAAIKHIRETQGVKLNVNLLGEAIL
                     GKDEAVRRLEGTRRLLLRDDVDYVSIKVSSTVAPHSPWAFDEAVAHAIKALHPLYEIA
                     AKPARGPKKFINLDMEEYKDLDLTIAVFTGILDRPEFKDLEAGIVLQAYLPDALSAMI
                     RLQEWAAERVADGGAPIKVRVVKGANLPMETVDAESHGWALATWSTKQESDASYKAVL
                     DYALRPEHIGNVRIGVAGHNLFDIAMAWLLASKRGVQEGIEFEMLLGMASAQAAVVKR
                     TVGSLLLYTPVVHPDEFDVAIAYLIRRLEEGASHENFMSAVFDLDADPKLFEREKERF
                     LASVAAMPTEVPGPNRTQNRQLPAAPAPRDGFVNTPDTDPSLAANREWADAIRARMVE
                     LKLGNDTVAANTLTTAEQVSERIATAVAAGEAWRALGAAGRAEILHRAGDVLEARRAE
                     LLEVMGSEAGKVIEQGDPEVSEAIDFAHYYAESAKALADVDGAEMHPVGLTVVTPPWN
                     FPVAIPAGSTLSALATGSPVVIKPARQARRSGAVMIEALW"
     gene            2084..>2274
                     /locus_tag="VN041_00295"
     CDS             2084..>2274
                     /locus_tag="VN041_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU27518.1"
                     /translation="MFELRRLRLLHELALRGTIAEVAASLSYSPSTVSQQLSLLEREA
                     GVALLEPDGRRVRLTPQGR"
BASE COUNT          322 a          722 c          872 g          358 t
ORIGIN      
        1 cccacagcgc ctcgatcatg acggcgccgg agcggcgcgc ctgacgggcg ggcttgatga
       61 ccaccggcga accggtggcc agggccgaga gggtggagcc ggcggggatc gcgaccggga
      121 agttccacgg cggggtgacc acggtcaggc cgaccgggtg catctcggcg ccgtcgacgt
      181 cggcgagcgc cttggcgctc tcggcgtagt agtgcgcgaa gtcgatggcc tcggagacct
      241 ccgggtcgcc ctgctcgatg acctttccgg cctcggagcc catgacctcg agcagttcgg
      301 cacggcgcgc ctcgagcacg tcaccggcgc ggtgcaggat ctcggcacga ccggcggcgc
      361 cgagcgctcg ccaggcctcg cccgcggcga cggcggtcgc gatgcgctcg gagacctgct
      421 cggccgtggt gagggtgttc gcggcgacgg tgtcgtttcc gagcttcaac tcgaccatgc
      481 gggcgcggat cgcgtcggcc cactcgcggt tggccgccag cgacgggtcg gtgtcggggg
      541 tgttcacgaa gccgtcgcgc ggcgccggcg cggcgggcag ctgacggttc tgagtgcggt
      601 tcgggccggg cacctcggtc ggcatggcgg cgacggatgc caggaagcgc tccttctcgc
      661 gctcgaacag cttcgggtcg gcgtcgaggt cgaacacggc cgacatgaag ttctcgtggc
      721 tcgcaccctc ctccaggcgg cggatgaggt aggcgatcgc gacgtcgaac tcgtcggggt
      781 gcaccaccgg cgtgtagagc agcagcgagc cgacggtgcg cttcacgacg gcggcctgag
      841 cggatgccat gcccagcagc atctcgaact cgatgccctc ctgcacgccg cgcttggagg
      901 cgagcagcca ggccatcgcg atgtcgaaga ggttgtggcc ggcgacgccg atgcgcacgt
      961 tgccgatgtg ctcggggcgc agggcgtagt cgagcacggc cttgtacgag gcgtcggact
     1021 cctgcttggt cgaccaggtg gccagcgccc agccgtgcga ctcggcgtcg acggtctcca
     1081 tgggcaggtt ggcgcccttc acgacgcgca ccttgatggg ggcgccgccg tcggccacgc
     1141 gctcggcggc ccactcctgc aggcggatca tggccgagag ggcgtcgggc aggtaggcct
     1201 gcagcacgat gccggcttcg aggtccttga actcgggacg gtcgaggatg ccggtgaaga
     1261 ccgcgatcgt gaggtcgagg tccttgtact cctccatgtc gaggttgatg aacttcttgg
     1321 ggccgcgggc gggcttggcg gcgatctcgt agagcgggtg cagcgccttg atggcgtgcg
     1381 cgacggcctc gtcgaacgcc cacgggctgt gcggggcgac ggtggaggag accttgatgg
     1441 agacgtagtc cacgtcgtcg cggagcagca ggcggcgggt gccctccagg cggcggacgg
     1501 cctcgtcctt gccgaggatg gcctcgccga gcaggttgac gttgagcttg acgccctggg
     1561 tctcgcggat gtgcttgatc gcggcgccca gcttctcgtc gcgggcgtcg acgatcaggt
     1621 ggcgcaccat ctggcgcagc acggcgcgtg cggaggggac gacgataccg gggagcatgg
     1681 tggcggtggc gccgcccagg ccgatcgccc ccttcatgat gccggggagg aaggcggggg
     1741 tcagcggcac gagctccttg agcttcgccg cggcgacctt caggtcctcg gggcgcacga
     1801 cgccgtcgac gaagccgacg gtgaagtcca ggccgttggg gtcgcgcagc acgccggcga
     1861 ggcgctggcc ggcggcatcg accggaacct cacggctgtc gaccagccac ttcttgacga
     1921 gggcgacggc gtcgtcggcc agttcggcga ggtcgttgac agatgcttcg gaagcggaca
     1981 caggggtgct catgctccca ggatgccgcg ttcgaacctc tatgaaaagc gatgattgat
     2041 gaaggataat gttcgttgtc atcgatgaat cggagggatg gggatgttcg agctgagacg
     2101 cctgcggctg ctgcacgaac tggcgctgcg cggcacgatc gccgaggtcg ccgcatcgct
     2161 ctcctactcc ccctcgacgg tgtcgcagca gctctcgctg ctggagcgcg aggccggcgt
     2221 ggccctgctc gagccggacg gccggcgggt gcggctgaca ccgcaggggc ggat
//
LOCUS       DATMSX010000009         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_38050,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000009 DATMSX010000000
VERSION     DATMSX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38050"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VN041_00300"
     CDS             <1..372
                     /locus_tag="VN041_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecQ"
                     /protein_id="HWU27519.1"
                     /translation="CVRVLAGWDWDERPVAVVGTPSRSRPQLTGTLARGFAEIGRLPY
                     LGELGFSGGGPTGGPGGNSAFRLAGVWDRFSADDLQVPEGPVLLVDDIADSRWTLTVA
                     ARTLRRAGATAVLPFTLAMRG"
     gene            complement(443..1675)
                     /locus_tag="VN041_00305"
     CDS             complement(443..1675)
                     /locus_tag="VN041_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU27520.1"
                     /translation="MIAPIVVVGGGHGGFHLALALREAGVQTPVILVDARDGLPVQRP
                     PLSKAILANGDATTRGRAQASLPFRPADFYARHDIRLRHATVVEIDRPCHEVRLADGD
                     RIAYSHLVLATGARNRRPDIPGVDLPGVVELRTPEDALHLTGALDDEVDVVVVGGGFI
                     GLEVASAAAARGRRVTVLEALDRVMSRVVSAPISEHFQVLHAARGVEIRTGAFAAAIH
                     GDHRVAEVELADGTRVPAGLVVLGIGILPEDRLARDAGLSVSNGIDVDSTLLTSDPSI
                     SAIGDCAQFPLAGVRRRLESVQNAADHARTVALRLAGSPEPYCAVPWFWSEQFSSKLQ
                     IAGVASGEEDAVVRLDGPRGFSVLHLAGGRLTVVESVDRPADHVAARRLLASGAMVDA
                     DRARSAGFRLTDLLADAE"
     gene            complement(1672..1989)
                     /locus_tag="VN041_00310"
     CDS             complement(1672..1989)
                     /locus_tag="VN041_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWU27521.1"
                     /translation="MPLVTYRLNDDENVIDVPEGTSLMQAAIDGDVDGIVGECGGNAM
                     CATCHVYVLGDAVTRLPAMADDEAEMLQCTASERRENSRLSCQIKMSYALGSLAVELP
                     EEQ"
     gene            complement(1998..2915)
                     /locus_tag="VN041_00315"
     CDS             complement(1998..2915)
                     /locus_tag="VN041_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU27522.1"
                     /translation="MMLVSFGPAGAETPAVLVDEERLMPVAPILASHGLRANDWSELL
                     GIWPAVEPVLREAIARGSESTILRSSVRLGPPVARPATVIAIGFNYSTHGADVLGAPP
                     PVGEPIVFLKPVTALSGPEDPIVLPPETSQLDYEIELAVVIGRGGRRIASADAAQHIA
                     GYMVANDVSARDVAFGPGIEHPLLFQIARAKGFPTFCPTGPWLLTADEVPDASSLGMR
                     LSVNGEVRQRATTAQMTVGVADLIASVSATMELRPGDILLTGTPPGCGFQQDPPSYLR
                     HGDAIEATIDRLGVMRTPVNAESVPELRV"
     gene            complement(2912..4102)
                     /locus_tag="VN041_00320"
     CDS             complement(2912..4102)
                     /locus_tag="VN041_00320"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU27523.1"
                     /translation="MVTRRPARDDASRHLQALPDDRDVQAFWESVEAQLDDLAARLIA
                     EIRTELPSYQALPPQEQNRTVRELLGAMTLSLTTGELPRSERLQMIRASARRRAYYGM
                     PIYDVLAAFHFAHRGLWATFRSSPCATERLLVELVEPMAAWAQAMSQAVVDAYVAEQG
                     GRDPREKEMRSQLFDALDDPARDQTTVDVLRELAYDAEGEFTVVVSRHQAWTRENTAA
                     LQRSCRRYAGVLQIGARAGLAVLISQGMAIDDLASAAREIVGPVALGVGLPRTGLPGI
                     RASIVDARRSLEVAESTDDQVVRFADQWFFATLLHSAPELDPLLSAATASATVHPDLA
                     DAVLAFADAGYSLVGAGERLRLNANSVSYRLGRWKEHTGLDVRDFSDLAASMLAVRRA
                     RSAR"
     gene            complement(4096..4935)
                     /locus_tag="VN041_00325"
     CDS             complement(4096..4935)
                     /locus_tag="VN041_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWU27524.1"
                     /translation="MSGVWGPDGRRAAVSVTFDHLGEAAELQTGQLPADTVVGDHHSV
                     TVELPRLLEMLEAHEHHVTFFVEAWNFDVYPDAVRSIVAAGHEIGWHGWLHEPWYQSS
                     PEQIADALRLSLDAFERLGIRPRGARPPAGLLGGHSLDVLREAGFEYVSLAGSSFGTD
                     DGMPLLSYPWHTVDGSYYIPGFTRLRRPPGDEVVSPAQMLDAYRGVVDETVEAGGCVA
                     FVFHVPWQDDPERIDAIGTLIDTLRADERVWLASGGEIADWLRAHPEDFPKSGHRDEA
                     PAW"
     gene            complement(4932..6161)
                     /locus_tag="VN041_00330"
     CDS             complement(4932..6161)
                     /locus_tag="VN041_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWU27525.1"
                     /translation="MVTLPDTVMLDASDASFAADRYSVYRSLRETRPVAPTMVNGRRS
                     WLITGLAEVERVLKDPTCLVEPEPGAIPAHVGTGAASEFYRYSLPNMDPPTHTRLRKL
                     ASPAFAPRAMKAMRGWVEEIIVSGLDRLAELGDEVDFVHEYAARVPAMIACRLLHAPM
                     SDAETVLQRMPALNAVLSQSDISPQQLADADSAAQFYIDYIGDLVDSLRGTLDDEDAV
                     GALLNAEADGARLSRMELVITLVGFFIASYHTTMVAMTNAVNALLRHPDQYRQLTADP
                     SLAPLAWEETLRFDSPVHFIWRYAGPGLELNGIPIPEGDHLLLGLAAANRDSARFDDP
                     DRYDITRTENKHVAFTAGGHYCLGAPLSRLEGDILLRELPRRLPGMQLREEHLQRLPD
                     LTFPVITRLMVSPRGAR"
     gene            complement(6302..7594)
                     /gene="paaK"
                     /locus_tag="VN041_00335"
     CDS             complement(6302..7594)
                     /gene="paaK"
                     /locus_tag="VN041_00335"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HWU27526.1"
                     /translation="MTAPAADQLDPAERLSRPELEALQLERLQWTVRHAYENVPHYTR
                     KLDEAGVSPDDIRSLADVRLLPFTTKEDLRQNYPFGMFAVPMEQVRRVHASSGTTGRP
                     TVVGYTDADLSNWADLIARSLRASGIRPGMKVHNAYGYGLFTGGLGAHAGIERLGATV
                     IPMSGGQTARQVQLIQDFQPDAILCTPSYLLTIADAMEEAGIDPRSTSLKIAVLGAEP
                     WTNEMRAELERRLDIDALDIYGLSEVMGPGVGNECVETKDGPHIWEDHFLPEVVDENG
                     QPLPDGEIGELVFTSLTKQAFPVLRYRTRDLTRLLPGTARPAMRRMEKVTGRNDDMII
                     LRGVNIFPTQIEELVLGIEQLTPHFVIELTREGRMDAMAVRIERHPSLERDVCEAAAK
                     VLAERIKTHVGSSVAVRLEEPGALPRSEGKYKRVYDLR"
     gene            complement(7632..8021)
                     /gene="paaI"
                     /locus_tag="VN041_00340"
     CDS             complement(7632..8021)
                     /gene="paaI"
                     /locus_tag="VN041_00340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HWU27527.1"
                     /translation="MRAMMQRDAASASLGMVVELDEPGRAVVSMPVREDMLNGFAITH
                     GGLVFTLADTAFAIACNEDERVTVAAGADITFLKSTGAGQTLTATAVKRVVSGRTGLY
                     DVTVTDETGDLVAEFRGRSITTNRAAE"
     gene            complement(8018..>8620)
                     /locus_tag="VN041_00345"
     CDS             complement(8018..>8620)
                     /locus_tag="VN041_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWU27528.1"
                     /translation="GGEELGGIRAVLHHMQRTAVQGSPEMLTALTGVWHAGATAHADG
                     RHPFRKALSELRIGDQIVSASREVTLDDIETFAHFTGDTFYAHMDEKSAAANPFFPGR
                     VAHGYLLVSWAAGLFVDPEPGPVLANYGLENLRFITPVSPGDSIRVELTAKQITPRET
                     DEYGEVRWDAVIRNQKDELVATYDVLTLVSKEPVSAEVSA"
BASE COUNT         1339 a         2881 c         3061 g         1339 t
ORIGIN      
        1 tgcgtgcgcg tgctcgccgg ctgggactgg gacgagcgcc cggtggcagt ggtcgggacg
       61 ccatcgcgtt cgcgtccgca gctcaccggc acgctggcgc gcggtttcgc cgagataggg
      121 cggctgccgt acctgggtga gctcgggttc tcgggcggag gtcccaccgg aggtcccggc
      181 ggcaacagtg cgttccgcct ggccggcgtc tgggatcgct tcagcgccga tgatctgcag
      241 gtgccggagg gtcctgtgct gctggtcgac gacatcgccg acagtcgctg gaccctcacc
      301 gtcgccgccc ggacactgcg ccgtgccggc gcgaccgccg tgctgccgtt cacgctggcg
      361 atgcgggggt agggggactc gagcacggcg aacgcgactg tggatcgctg tccggtccgc
      421 ggacccgacg agacgcccga tctcactccg cgtcggcgag cagatccgtg aggcggaacc
      481 cggcacttcg cgcgcggtcg gcgtcgacca tggcgcccga ggcgagcagc cggcgagccg
      541 ccacgtggtc cgcaggccgg tccacggact ccacgaccgt taggcgcccg cctgcgagat
      601 ggagcacgga gaatcctctc gggccgtcga gacgcacgac cgcgtcctcc tcgcccgacg
      661 ccactccggc gatctggagc ttcgaggaga actgctcgct ccagaaccag gggacagcgc
      721 agtagggctc cggtgagccg gcgaggcgaa gcgcgaccgt gcgcgcgtga tccgcggcgt
      781 tctgcacgga ctcgagtcgg cggcgcacgc ctgcgagtgg gaactgggcg cagtcgccga
      841 tcgccgagat cgacggatcc gaggtgagca gcgtcgaatc gacgtcgatg ccgttggaca
      901 cggacagtcc ggcgtcgcgc gccagccggt cctcgggcag gatgccgatg ccgagcacca
      961 cgagccctgc cgggacccgc gtcccgtcgg cgagctccac ctcggcgaca cggtgatccc
     1021 cgtgaatcgc tgcggcgaag gcgccggtgc ggatctcgac gccgcgcgcc gcatgcagca
     1081 cttggaagtg ctccgagatc ggtgcggaca cgacacgtga catcacccga tcgagggcct
     1141 cgagcaccgt cacccgtcgt ccccgcgctg ccgccgcgga agcgacctcg aggccgatga
     1201 atccgccacc gaccacgacc acgtccacct cgtcgtcgag ggctcccgtg aggtgcagcg
     1261 catcttccgg cgttcgcagc tccacgaccc cgggaaggtc gacaccgggg atgtcgggcc
     1321 gtcgattgcg cgcgccggtg gccagcacga gatgggagta tgcgatccgg tcgccatcgg
     1381 cgaggcgcac ctcgtgacaa gggcggtcga tctccaccac cgtggcgtga cggagccgaa
     1441 tgtcgtgcct ggcatagaag tcggccggcc ggaagggcaa ggacgcctgc gcgcggccgc
     1501 gcgtggtcgc atcgccgttc gcgaggatcg ccttggagag cggaggtcgc tgcacgggaa
     1561 ggccgtcgcg cgcatccacg aggatcacgg gcgtctgcac ccctgcctcc cgcagggcca
     1621 gggcgagatg aaagccgccg tgaccgcctc cgacgaccac gatgggggcg atcactgctc
     1681 ctccggaagc tcgacggcca gagagcccag tgcgtaggac atcttgatct gacagctcaa
     1741 gcggctgttc tcccgtcgct ccgacgcggt gcactgcagc atctcggcct cgtcgtccgc
     1801 catcgcaggc agcctcgtca cggcgtcgcc gaggacgtag acgtggcagg tcgcgcacat
     1861 cgcgttgccg ccgcattcgc cgacgatgcc gtcgacgtca ccgtcgatcg cggcctgcat
     1921 gaggctcgtt ccctcaggaa catcgatcac gttctcgtcg tcgttcagac gataggtcac
     1981 aaggggcaca gcttctctca gactctcaat tcgggaaccg actcggcgtt caccggtgtg
     2041 cgcatcacgc ccagccggtc gatcgtcgcc tcgatggcgt cgccgtgacg gaggtatgac
     2101 ggtgggtcct gctggaaccc gcaccccggc ggagtaccgg tgagcaggat gtctccgggg
     2161 cggagctcca tcgtggcgct caccgaggcg atgaggtccg cgactccgac ggtcatctgc
     2221 gcggtcgtcg cgcgctgtcg gacctcgccg ttcacgctga gccgcatgcc cagggacgag
     2281 gcatccggga cctcgtcggc ggtgagcagc cacggaccgg tcgggcagaa ggtggggaac
     2341 cccttggctc gggcgatctg gaacagaaga ggatgctcga tgcccgggcc gaaggccaca
     2401 tccctcgccg acacgtcatt ggcgaccatg tagccggcga tgtgctgcgc ggcatcggct
     2461 gacgcgatgc gacggccacc gcggccgatg accacggcga gttcgatctc gtagtcgagc
     2521 tgcgatgtct cgggcggcag gacgatggga tcctccggac cactgagtgc agtcacgggc
     2581 ttgaggaaga cgatcggctc gcctaccggc ggcggagctc ccagcacgtc ggcaccgtgc
     2641 gtggagtaat tgaagccgat cgcgatcact gtcgccggac gtgccaccgg cggtccgagt
     2701 cgcaccgacg atcggaggat cgtcgactcg gagccccggg cgatggcctc gcgcagcacg
     2761 ggctcaacgg cgggccagat gccgagcagc tcagaccagt cgttcgcgcg cagaccatgc
     2821 gaagccagga tcggggcgac cggcatcagg cgttcctcgt cgacgagcac ggcgggcgtc
     2881 tcggcacccg cgggcccgaa gctcacgagc atcatcgtgc gctccgtgcg cgcctgacgg
     2941 ccagcatcga ggcagcaaga tcggagaagt cgcgcacgtc gaggccggtg tgctccttcc
     3001 accgtccgag tcggtacgag acggagttcg cgttcaggcg caggcgttcg cccgcgccga
     3061 ccaaggagta gcccgcgtcc gcgaacgcca gcacggcgtc cgcgagatcc gggtgcactg
     3121 tcgcggatgc cgtcgcagcc gagagcagag gatccaactc gggcgccgag tgcagcagag
     3181 tcgcgaaaaa ccactgatcc gcgaaccgca cgacctggtc gtcggtggac tcggcgactt
     3241 cgagtgagcg ccgtgcatcg acgatgctcg cacggatgcc gggcagcccg gtgcgcggga
     3301 ggccgacgcc cagggcgacg ggaccgacga tctcccgcgc ggcggatgcc agatcgtcga
     3361 tggccatgcc ctgcgagatc aacaccgcca gccccgcgcg ggcaccgatc tgcagtacgc
     3421 cggcgtaccg gcggcacgag cgctgcaatg cggccgtgtt ctcgcgggtc caggcctggt
     3481 ggcgggagac gacgacggtg aactcgccct cggcgtcgta ggcgagctcg cgcagaacgt
     3541 ccaccgtggt ctggtcccgg gcggggtcgt cgagagcgtc gaacagctgc gagcgcatct
     3601 ccttctcgcg cggatcacgc ccgccctgct ccgccacgta ggcgtcgacg acggcttggc
     3661 tcatcgcctg ggcccacgcg gccataggct cgaccagctc caccagcagg cgctcggtcg
     3721 cgcacggaga ggagcggaac gtcgcccaca gtccgcgatg tgcgaagtgg aaagcggcca
     3781 ggacgtcata gatgggcatg ccgtagtagg cgcgtctgcg cgcggaggcg cggatcatct
     3841 gcaggcgttc agaccggggc agttcgccgg tggtgaggct gagcgtcatc gcgcccaaca
     3901 gctcgcgcac cgtgcggttc tgctcctgcg gtggcagcgc ctgatatgag ggcagctcgg
     3961 ttcggatctc ggcgatgagg cgtgctgcca gatcgtcgag ctgcgcctcc acgctctccc
     4021 agaaggcctg aacatcccga tcgtccggga gggcctgaag gtgccgtgag gcgtcgtcac
     4081 gggccggtcg acgcgtcacc atgccggagc ctcgtcacgg tgcccggact tcgggaagtc
     4141 ctccgggtgg gcgcgcagcc agtcggcgat ctcgccgcca gaggccagcc agacccgctc
     4201 atcggcgcgg agcgtgtcga tcagggtgcc gatcgcgtcg atcctctcgg ggtcgtcctg
     4261 ccacggcacg tggaacacga aggcgacgca tccgccggcc tccaccgtct cgtccacgac
     4321 gcctctgtat gcgtccagca tctgtgcggg actcaccact tcgtcgcccg gagggcggcg
     4381 aagccgggtg aatcccggaa tgtagtacga gccgtccacg gtgtgccagg ggtacgacag
     4441 caacggcatc ccgtcgtcgg tgccgaagct ggaacccgca agcgagacgt actcgaagcc
     4501 ggcttcgcgc agcacgtcga gcgagtgccc gcccagaagg ccggccggcg ggcgcgcgcc
     4561 gcgcggccgg atgcccagcc gttcgaacgc atccaggctc agacgcagcg catccgcgat
     4621 ctgttccggc gagctctgat accagggctc gtgcagccac ccatgccagc cgatctcgtg
     4681 accggccgcc acgatggagc gcacggcgtc ggggtagaca tcgaaattcc acgcctcgac
     4741 gaagaacgtg acgtgatgct cgtgcgcttc cagcatctcc agcagtcggg gcagttcaac
     4801 ggtcacgctg tggtgatcgc cgaccacggt atccgccgga agctgaccgg tctgcagctc
     4861 ggcggcctcg ccgagatgat cgaaggtgac gctgacagcg gcacggcgcc cgtccggacc
     4921 ccacacgccg ctcatcgcgc acccctcggc gagaccatca gccgggtgat gacggggaat
     4981 gtcaggtcgg ggaggcgctg caggtgctcc tcccggagct gcatgcccgg tagacggcgg
     5041 gggagctcgc gcagcaggat gtcgccttcc agccgggaga gcggagcacc caggcagtag
     5101 tgtccgcctg ccgtgaacgc gacgtgcttg ttctcggtgc gggttatgtc gtagcggtcg
     5161 gggtcgtcga agcgggcgct gtcgcggttg gctgcggcca gccccagaag aaggtggtcg
     5221 ccctcgggga tcgggatgcc gttgagctcg agcccgggac cggcgtagcg ccagatgaag
     5281 tgcacagggg agtcgaagcg gagcgtctcc tcccaggcga gcggcgcgag ggagggatcg
     5341 gcagtgagct gccggtactg gtccggatgg cgcagcagag cgttcacggc attcgtcatg
     5401 gcgaccatcg tcgtgtggta gctggcgatg aagaagccca ccagggtgat gacgagctcc
     5461 atgcgcgaca gtcgcgcgcc atccgcctct gcgttcagca gggccccgac ggcgtcctcg
     5521 tcgtcgagcg tgccgcgcag actgtcgacg aggtcgccga tgtagtcgat atagaactgc
     5581 gcggcggagt ccgcgtccgc cagctgctga ggcgagatgt cgctctgact cagcaccgcg
     5641 ttcagcgcgg gcatccgctg gagcaccgtc tcggcatccg acatgggggc gtgcagcagc
     5701 cggcaggcga tcatggcggg cacgcgcgcg gcatactcgt gaacgaagtc gacctcgtct
     5761 cccagctcgg ccagcctgtc gagacctgac acgatgatct cctccaccca gccgcgcatg
     5821 gccttcatcg cgcgcggcgc gaacgcgggc gaggcgagct tgcgaagcct ggtgtgggtg
     5881 ggcgggtcca tgttgggcag cgagtaccgg tagaactcgg atgccgcgcc cgtgcccaca
     5941 tgcgcgggta tcgctccggg ttcgggctcg acgaggcacg tcgggtcctt gagcacccgc
     6001 tcgacctcgg cgagtccggt gatgagccag gaacgccggc cgttgaccat cgtcggtgcc
     6061 accggtcgcg tctcgcgaag cgatctgtag accgaatagc ggtcggcggc gaacgaggca
     6121 tcggatgcat ccagcatcac ggtgtcgggc agagtgacca tatgcgtctc cacggtgaga
     6181 tcgatgggcg gatcgctcgg gtgcgttccg cgttgtctac atcgagccta ggaagagtgc
     6241 ggtccggttt cctctcgtga acgtcaagat gtcagggtgg atcttgcgcg atccgcaaac
     6301 atcagcgcag gtcgtacacc cgcttgtact tgccctcgct gcgggggagt gctccgggct
     6361 cctccagtcg caccgcgacg ctggagccga cgtgcgtctt gatgcgttcg gccagcacct
     6421 tcgctgcggc ctcgcacacg tcgcgctcca ggctcgggtg ccgttcgatg cgcaccgcca
     6481 tggcgtccat ccggccctcc cgggtcagct cgatgacgaa atgcggggtg agctgctcga
     6541 tgcccagcac gagctcctcg atctgagtgg ggaagatgtt cacgccgcgc aggatgatca
     6601 tgtcgtcgtt gcgcccggtg accttctcca tgcggcgcat cgccggtcgc gcggtcccgg
     6661 gaagcaggcg ggtcaggtct cgtgtgcggt agcgcagcac cgggaacgcc tgcttggtca
     6721 gcgaggtgaa caccagctcg ccgatctcgc cgtctggcag gggctgcccg ttctcgtcga
     6781 ccacctcggg caggaagtgg tcctcccaga tgtgcgggcc gtccttggtc tccacgcact
     6841 cgtttccgac gccagggccc atcacctcgc tcaggccgta gatgtccagc gcatcgatgt
     6901 ccaggcgacg ttccagctcg gcgcgcatct cgttggtcca cggctcggcg ccgagcaccg
     6961 cgatcttcag cgacgtcgac ctcgggtcga tgcccgcctc ctccatcgcg tcggcgatcg
     7021 tgaggagata actgggggtg cagaggatgg catccggctg gaagtcctgg atgagctgca
     7081 cctgtcgcgc ggtctggcct cccgacatcg ggatgaccgt cgcacccagc cgctcgatac
     7141 cggcgtgagc gcccagcccg ccggtgaaca ggccgtatcc gtacgcgttg tgcaccttca
     7201 tgccggggcg gatgccggag gcgcgcagcg atctggcgat gaggtctgcc cagttcgaca
     7261 ggtcggcgtc ggtgtagccg accacggtcg ggcggcccgt cgtgcccgag gaggcgtgca
     7321 cgcggcgcac ctgctccatc ggcacggcga acatgccgaa cgggtagttc tgtcgcaggt
     7381 cctccttggt ggtgaagggc agcagtcgca cgtcggcgag agaccggatg tcgtcgggcg
     7441 agacgccggc ctcgtcgagc ttgcgcgtgt agtgcggcac gttctcgtag gcgtggcgga
     7501 cggtccattg caggcgctcc aactggagcg cctccagctc gggccgggag agtcgctcgg
     7561 cggggtcgag ctggtctgct gcgggtgctg tcatcgtcga catcgggtgc tccggggtga
     7621 gggtcagcgc gtcattcggc ggcgcggttg gtggtgatgg atcggccgcg gaactcggcg
     7681 accaggtcgc cggtctcgtc ggtcaccgtc acgtcgtaca ggccggtgcg tccgctcacg
     7741 acgcgcttga cggcggtcgc cgtcagggtc tggccggcac cggtcgactt gaggaaggtg
     7801 atgtcagcgc cggcggcgac ggtcacgcgc tcgtcctcgt tgcaggcgat cgcgaacgct
     7861 gtgtcggcga gcgtgaacac gagtccgccg tgggtgatgg cgaagccgtt gagcatgtcc
     7921 tcgcgcaccg gcatcgagac gacggcccgc cctggctcgt cgagctccac caccatgccg
     7981 agcgacgccg acgcggcatc ccgctgcatc atcgccctca tgccgacacc tctgccgaga
     8041 ccggctcctt gctcaccagc gtgagcacgt cgtaggtggc gaccagctca tccttctggt
     8101 tgcggatcac ggcatcccag cgcacctcgc cgtactcgtc ggtctcgcgc ggggtgatct
     8161 gcttggcggt caactcgacg cggatgctgt cgccggggga gaccggggtg atgaaccgca
     8221 ggttctccag accgtagttg gcgagaacgg ggccgggctc cgggtcgacg aacaggccgg
     8281 ccgcccacga gacgagcagg tagccgtgtg cgacccggcc ggggaagaac gggttcgccg
     8341 cggccgactt ctcgtccatg tgggcgtaga aggtgtcgcc ggtgaagtgc gcgaaggtct
     8401 cgatgtcgtc cagggtgacc tcacgcgacg ccgacacgat ctggtcgccg atgcgcagct
     8461 ccgagagcgc cttgcggaac ggatgccggc catcggcgtg cgcggtcgca ccggcgtgcc
     8521 acacaccggt cagtgcggtg agcatctcgg gggagccctg caccgcggtg cgctgcatgt
     8581 ggtgcagcac agcgcggatg ccgccgagct cctcgccgcc
//
LOCUS       DATMSX010000010         7611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123362,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000010 DATMSX010000000
VERSION     DATMSX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123362"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2345)
                     /locus_tag="VN041_00350"
     CDS             complement(<1..2345)
                     /locus_tag="VN041_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWU27529.1"
                     /translation="MIDRERHPVDPWRLIETRYDDDGVSETLFSVGNGYLGLRGNHIE
                     GRGAHEHGTFINGLHETWPIRHAEQAYGFAEVGQTIVNAPDAKVMRVYIDDEPLSFDE
                     TEIHEYSRVLDMRTGVLSRTVVWVTPSGKRVRMHDERIVSFDERHLAVLRLEVVVENA
                     DAPVTISCQLLNRQDGAGIYAGDPMASKSEGKAGFDPRKAEKITERVLQPVEYWQDGL
                     RSALSYRVNDSGMSVAVVADHLIETENDYSARTLIEPDIAKNVFRVQAKAGVPIRISK
                     IVSYHSSRGVPPRELVDRCRRSLDRVGVEGVDALFHAQEAWLAAFWERSDVRIAGHDD
                     LQQATRWCLFQLAQAASRADGTGVPAKGLTGSGYSGHYFWDTEIYVLPFLTYTSPQWA
                     KNALRARVLMLPAARRRAAQLNEAGALFPWRTINGEEASAYYAAGTAQYHINADVSYA
                     LGKYVRATGDEEFLRREGADIAVETARLWATLGFWRGTNGHSTFHIHGVTGPDEYTTV
                     VNDNLFTNVMARYNLRLAARVVREMAEQDPSAYAALVERTGLDAGEREAWDRAAEAIH
                     IPFSEALRIHPQDSLFLEREVWDLEGTSPDQRPLLLHYHPLVIYRFQVLKQADVVLAL
                     FLQGNHFTDEEKLADFDYYDPLTTGDSTLSAVVQSIMAAEVGYQDLAREYFEQALFVD
                     LGNLHHNSSDGVHVASAGGVWTALVSGFGGMRDHDGLLSFDPRLPADWPELSYALQWH
                     GSQLQVTVTREALRLEVRSGDPVDFSVRGVAYRASVAEPAVV"
     gene            complement(2380..3519)
                     /locus_tag="VN041_00355"
     CDS             complement(2380..3519)
                     /locus_tag="VN041_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HWU27530.1"
                     /translation="MSAELLIDRRGDALTYRVQDDGVELAHGTVDGVGRPLAHIVHTV
                     RPPVRPGEPAPTVLSIRHRTEQTLADTDAAVAAILRICAEKGPALGETPRVVLATLGG
                     SATPDPSAMAAEAPVPDNRTVPRSPIVSEDLPDLRRAAGVLFDLDGVLTPTAEVHMRA
                     WQAVFDEVLTRWGVTEPYTDADYFAYVDGKKRYDGVASLLRSRNVEIPWGEVTDPPEA
                     ETICGVGNRKNAAFAAALRSEGIAPYPGSLALLEQLRAAEVPMGVVSSSKNAPEVLAS
                     AGIRDFFRIVVDGVVAEHEHLASKPAPDMFREGARMLGVDPSEAIAVEDAVSGVASAA
                     SAGYATVVGVDRGAGADALREAGATCIVDDLARLLSGAEPGGDPH"
     gene            complement(3516..4550)
                     /gene="moaA"
                     /locus_tag="VN041_00360"
     CDS             complement(3516..4550)
                     /gene="moaA"
                     /locus_tag="VN041_00360"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWU27531.1"
                     /translation="MALQLGSRLRDTRGRMLRDLRISVTDRCNFRCVYCMPKEVFGRD
                     HDFLPHAMLLSFEEIVRVAKAGVAHGVHKLRLTGGEPLLRRDIETLIAMLAELRTPEG
                     ERLDIALTTNGSALAYKAQALRDAGLNRVTVSLDSLDDATFRAMNDIDFPLERVLDGI
                     RTAEQVGFDAIKVNTVVKRGTNEGDVLGIAERFRGTGVTVRFIEFMDVGTTNGWNLDQ
                     VVSSAEIIERIGAVHPLEPLQPAYRGETATRWRYLDGAGEIGAISSVTAPFCGTCSRA
                     RISADGKLFTCLFASGGHDLRALLRGGCSDAELEAALAQIWGARDDRYSEMRSADTAV
                     ERIEMSYIGG"
     gene            complement(4600..6900)
                     /locus_tag="VN041_00365"
     CDS             complement(4600..6900)
                     /locus_tag="VN041_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HWU27532.1"
                     /translation="MAAKAPIQDISDDARDAEVGPPKEWAAGVPGVLHSMVPGIREMG
                     VRRALTLLTDMNQKDGFDCMSCAWADPDHRKTFEFCENGAKAVTWEATPVTIPTAFWA
                     QNSISSLLTKSEYWLGMQGRLVEPVYKPQGQDNYRPISWDEAFTLIADRLKAMDSPDE
                     ATFYTSGRTANETAFIYQLMARTLGTNNLPDCSNMCHESTGTALGEVIGIGKSTISYD
                     DFGKADLIIVMGQNPGTNHPRMLTALEEAKDNGAKIVAVNTLHEAGLTRYKNPQRVRG
                     IIGHGTGLADQYLLIRSGGDGWLMKAVSKKVLEAEDAAPGTVLDHAFLDEHCQGLEEF
                     REHVRTLDEDRVLQATGLSQAEIDELADRYLNSERVIITWAMGLTQHRDAVKILKEVM
                     NLLFLRGNIGKPGAGASPIRGHSNVQGDRTMGIWEQMPESFLAALDREFGITVPREHG
                     YDVVKSVQAMREGRTRVWMAMGGNMVAAVSDTHAAEEAFRNVGLNVQVSTKLNRNHLT
                     VGDEAIILPVLGRTEIDRQEAGEQSLSVEDSVCAIHATRGKVEPISDQLKSEVSVVVG
                     IARALWGDGGPIDWAGFEKDYDRIRDHIEHVVPGFDDFNARLREEHGFILPHGPRDSR
                     TFPTTTGKAMVTVNQLEYLECPPGRLLLQSMRAHDQFNTTIYGLDDRYRGIRKSRRVI
                     FVNPDDLRDLGIADGQIVDVHSEYEDGVDRVVHEFKAVSYPTSRGCAATYFPEGNPLV
                     PLGLTAFGSNTPASKGVVVRLEPVTR"
     gene            complement(6985..>7611)
                     /gene="fdhD"
                     /locus_tag="VN041_00370"
     CDS             complement(6985..>7611)
                     /gene="fdhD"
                     /locus_tag="VN041_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWU27533.1"
                     /translation="YALGASAADGGNTYNVLDVTLAPGTPLPDPSRARHSYTTSACGV
                     CGAASIEQVATTSAFDVSADPLVVDAATLADLPNMLRSRQEVFEKTGGLHAAGLYDSA
                     TGELLVVREDVGRHNAVDKVVGWAVLNGRLPLTGTVLQVSGRASFELVQKAVMAGIPM
                     LSAVSAPSSLAVEMAESTGLTLVGFVRGATMNVYSRPERVREAVPAAV"
BASE COUNT         1086 a         2666 c         2625 g         1234 t
ORIGIN      
        1 accacagccg gctcggcgac ggatgcccgg tacgcgaccc cgcgcacgct gaagtccacc
       61 ggatcgcccg agcgcacctc gagtcgcagc gcctcgcgcg tcacggtcac ctgcagctgc
      121 gagccgtgcc actgcagcgc gtacgacagc tcgggccagt cggccggcag gcgcgggtcg
      181 aagctcagca gtccgtcgtg gtcgcgcatg ccaccgaagc cgctgaccag cgcggtccac
      241 acaccgccgg cggacgccac gtgcacgccg tccgaggagt tgtggtgcag gttgcccagg
      301 tcgacgaaca gcgcctgctc gaagtactcg cgcgccagat cctggtatcc gacctccgcc
      361 gccatgatcg actgcaccac ggccgacagt gtcgagtcac cggtggtcag cgggtcgtag
      421 tagtcgaagt cggcgagctt ctcctcatcg gtgaagtggt tgccctgcag gaacagcgcc
      481 agcacgacgt ccgcctgctt gagcacctgg aaccggtaga tcaccagcgg gtggtagtgc
      541 agcagcagcg ggcgctggtc gggcgaggtg ccctcgagat cccacacctc gcgctccagg
      601 aacagcgagt cctgcgggtg gatgcgcagc gcctcgctga acgggatgtg gatcgcctcg
      661 gctgcccgat cccaggcctc gcgctccccg gcatccagac cggtgcgctc caccagcgcg
      721 gcgtaggccg acggatcctg ctcggccatc tcgcgcacca cgcgcgcggc cagccgcagg
      781 ttgtaccgcg ccatcacgtt cgtgaacagg ttgtcgttga cgaccgtggt gtactcgtcc
      841 ggcccggtga cgccgtggat gtggaacgtc gagtggccgt tggtgccacg ccagaagccc
      901 agcgtcgccc acagccgcgc ggtctcgacg gcgatgtcgg ccccctcccg gcgcaggaac
      961 tcctcgtcgc cggtggcgcg cacgtacttg ccgagcgcat agctgacgtc ggcgttgatg
     1021 tggtactgcg cggtgcccgc cgcgtagtac gccgacgcct cctcgccgtt gatcgtgcgc
     1081 cacgggaaca gggcgcccgc ctcgttgagc tgggcggcgc ggcggcgggc ggcgggcagc
     1141 atgagcaccc gggcgcgcag cgcgttcttc gcccactgcg gcgacgtgta ggtgaggaac
     1201 ggcaggacgt agatctcggt gtcccagaag tagtggccgc tgtagcccga gccggtcagg
     1261 cccttcgccg ggacgccggt gccatcggca cgggacgccg cctgcgcgag ctggaacagg
     1321 caccaccggg tggcctgctg caggtcgtcg tggccggcga tgcgcacgtc cgaacgctcc
     1381 cagaacgccg cgagccacgc ctcctgggcg tggaacaggg cgtcgacgcc ctccactccc
     1441 acacggtcca gcgaacggcg gcagcggtcg acgagctcac gcggcggcac gccccgcgag
     1501 ctgtggtagc tgacgatctt gctgatccgg atcggcaccc cggccttcgc ctgcacgcgg
     1561 aacacgttct tggcgatgtc cggctcgatg agcgtgcggg cgctgtagtc gttctcggtc
     1621 tcgatgaggt gatcggccac gaccgcgacg ctcatgcccg agtcgttcac ccggtacgac
     1681 agcgcggagc gcagcccgtc ctgccagtac tccaccggct gcagcacgcg ctcggtgatc
     1741 ttctccgcct tgcgcgggtc gaagcccgcc ttgccctcgg acttcgacgc catcgggtcg
     1801 ccggcgtaga tgcccgcgcc gtcctggcgg ttcagcagct ggcagctgat cgtgacgggg
     1861 gcgtcggcgt tctccacgac gacctcgagg cgcagcaccg ccaggtgccg ctcgtcgaag
     1921 ctgacgatcc gctcgtcatg catgcgcacg cgcttgcccg acggcgtcac ccacaccacg
     1981 gtgcgcgaca gcacgccggt gcgcatgtcg agcacccggg agtactcgtg gatctcggtc
     2041 tcgtcgaagg acagcggctc gtcgtcgatg tagacgcgca tcaccttggc atcaggcgcg
     2101 ttgacgatcg tctgccccac ctcggcgaac ccgtacgcct gctcggcgtg gcggatcggc
     2161 cacgtctcgt gcagaccgtt gatgaacgtg ccgtgctcgt gcgcaccgcg tccctcgatg
     2221 tggttgccgc gcaggccgag atagccgttg ccgaccgaga agagcgtctc ggacacgccg
     2281 tcgtcgtcgt agcgggtctc gatcaggcgc caggggtcca cggggtggcg ttcgcggtcg
     2341 atcatgcgtt ctccgaaggg attctgaagg gtgaggggct cagtgcgggt cgcccccggg
     2401 ttcagcaccg gagagcagac gcgcaagatc atcgacgata caggtggcgc cggcctcgcg
     2461 cagcgcgtcg gcgcctgcgc cgcggtcgac gccgacgacg gtcgcgtaac cggcggatgc
     2521 cgcggatgcc acaccggaga ccgcatcctc cacggcgatc gcctcggagg ggtcgactcc
     2581 cagcatccgg gcgccctcgc ggaacatgtc gggggcgggc ttggacgcga ggtgctcgtg
     2641 ctcggccacg acgccgtcga cgacgatgcg gaagaagtct cggatgccgg cggatgccag
     2701 cacctcgggc gcgttcttcg agctggagac cacgcccatc gggacctcgg cggcgcggag
     2761 ctgctccagc agcgcgagcg aaccggggta cggcgcgatg ccttcgctgc gcagcgcggc
     2821 ggcgaacgcg gcgttcttgc ggtttccgac gccgcagatc gtctccgcct cgggcgggtc
     2881 ggtgacttcg ccccacggga tctcgacgtt gcggctgcgc agcaggctgg ccacgccgtc
     2941 gtagcgcttc ttgccgtcga cgtacgcgaa gtagtcggcg tccgtgtacg gctcggtgac
     3001 gccccagcgg gtcagcactt cgtcgaacac ggcctgccat gcgcgcatgt gcacttcggc
     3061 ggtcggcgtg agcacgccgt ccaggtcgaa cagcactccc gcagcgcggc gcaggtcggg
     3121 caggtcttcg gacacgatgg gactcctggg gacggtgcgg ttgtcgggca cgggtgcttc
     3181 ggcggccatg gcgctcggat ccggtgtggc gctccctcca agcgtagcga gaaccacccg
     3241 cggggtctcc cccaacgcgg ggcccttctc ggcgcagatc cgcaggatcg ccgcgaccgc
     3301 ggcatccgtg tcggcgaggg tctgctccgt gcggtgccgg atgctgagca ccgtcggcgc
     3361 cggttccccc ggacgcaccg gcggacgcac ggtgtggacg atgtgcgcga gcggccggcc
     3421 gaccccgtcg accgtgccgt gcgcgagctc gaccccgtcg tcctgcacgc ggtatgtcag
     3481 cgcgtcaccg cgccggtcga tcagcagctc cgcgctcatc cgccgatgta cgacatctcg
     3541 atgcgctcca ccgcggtgtc cgcggagcgc atctcggagt agcggtcgtc gcgtgcgccc
     3601 cagatctgcg ccagggcggc ctccagctcg gcatccgagc atccgccgcg cagcagcgcg
     3661 cgcaggtcgt gcccgccgga cgcgaacagg caggtgaaca gcttgccgtc ggccgagatg
     3721 cgcgcacggc tgcaggtacc gcagaacggc gccgtgacgc tggagatggc gccgatctcc
     3781 cccgccccgt cgaggtagcg ccagcgcgtc gccgtctcgc cccggtaggc gggctgcaac
     3841 ggctcgagcg gatgcacggc gccgatccgc tcgatgatct cggccgacga caccacctgg
     3901 tcgaggttcc agccgttcgt ggtgccgaca tccatgaact cgatgaagcg cacggtcacc
     3961 ccggtgccgc ggaagcgctc ggcgatgccg agcacgtcgc cctcgttcgt gccgcgcttg
     4021 accaccgtgt tgaccttgat cgcgtcgaag ccgacctgct cggcggtgcg gatgccgtcg
     4081 agcacccgct ccagcgggaa gtcgatgtcg ttcatggcgc ggaacgtcgc gtcgtcgagg
     4141 gagtccagcg agaccgtgac gcgattcagc ccggcgtcgc gcagcgcctg cgccttgtac
     4201 gcgagagccg agccgttggt ggtcagggcg atgtcgaggc gctccccctc gggggtgcgc
     4261 agctcggcca gcatcgcgat cagcgtctcg atgtcgcggc gcagcagcgg ctcgccgccg
     4321 gtcagccgca gcttgtgcac gccgtgtgcg acgcccgcct tcgcgacgcg cacgatctcc
     4381 tcgaagctca gcagcatcgc gtgggggagg aagtcgtggt cgcggccgaa gacctccttg
     4441 ggcatgcagt acacgcagcg gaagttgcag cggtcggtga ccgagatgcg caggtcgcgc
     4501 agcatccgcc cgcgggtgtc ccgcaggcgt gatccgagct gcagtgccat gtcctccacc
     4561 gtagtcgtcg tgtccgtgcc cgcggccgga tcggaccgct cagcgggtga cgggctccag
     4621 ccggacgacc acgcccttcg atgcgggcgt gttgctgccg aacgccgtca ggcccagcgg
     4681 caccagcggg ttcccctccg ggaagtacgt cgcagcgcat ccgcgcgatg tgggatagga
     4741 gacggccttg aactcatgca cgacacggtc gacgccgtcc tcgtactcac tgtgcacatc
     4801 cacgatctgg ccgtcggcga tgccgagatc cctgaggtcg tcggggttca cgaagatcac
     4861 acggcggctc ttccggatgc ctcggtagcg atcgtcgaga ccgtagatcg tcgtgttgaa
     4921 ctggtcgtgc gcgcgcatcg actgcagcag gagccggccc ggagggcact ccaggtactc
     4981 cagttgattc acggtgacca tcgccttgcc ggtcgtcgtc gggaacgtcc gggagtcgcg
     5041 cggcccgtgc gggaggatga acccgtgctc ctcgcgcagc cgcgcgttga agtcgtcgaa
     5101 cccggggacg acatgctcga tgtgatcccg gatccggtcg tagtccttct cgaacccggc
     5161 ccagtcgatc gggccgccat cgccccacag cgcccgcgcg atgccgacga ccaccgagac
     5221 ctccgacttg agctgatcgg agatcggctc gaccttgccc cgggtggcgt ggatcgcgca
     5281 caccgagtcc tccaccgaca gcgactgctc gccggcctcc tgccggtcga tctcggtgcg
     5341 tccgagcacc ggcaggatga tcgcctcgtc ccccacggtg aggtggttgc ggttgagctt
     5401 ggtggagacc tgcacgttga gcccgacgtt gcggaaggcc tcctcggcgg cgtgcgtgtc
     5461 ggagacggcc gcgaccatgt tgccgcccat cgccatccac accctggtcc tgccctcgcg
     5521 catcgcctgc accgacttga ccacgtcgta gccgtgctcg cgcgggaccg tgatgccgaa
     5581 ctcccggtcc agggccgcga ggaagctctc cggcatctgc tcccagatgc ccatggtccg
     5641 gtcgccctgc acattgctgt ggccgcggat cggcgaggcg ccggcacccg gcttgccgat
     5701 gttcccgcgc aggaacagca ggttcatgac ctccttcagg atcttcaccg catcccggtg
     5761 ctgcgtgagc cccatcgccc aggtgatgat gacccgctcg gagttcaggt agcggtcggc
     5821 gagctcgtcg atctcagcct gggagaggcc ggtggcctgc agcaccctgt cctcgtcgag
     5881 ggtcctgacg tgctcacgga actcctccag tccctgacag tgctcgtcga ggaaggcgtg
     5941 gtcgagcacg gtccccggtg cggcgtcctc cgcctcgagg accttcttcg agacggcctt
     6001 catcagccag ccgtcgccgc ccgaccggat gagcaggtac tggtcggcca gacccgttcc
     6061 gtggccgatg atgccgcgca cccgctgcgg gttcttgtag cgggtcagcc ccgcctcgtg
     6121 cagcgtgttg acggcgacga tcttcgcccc gttgtccttg gcctcctcca gcgcggtgag
     6181 catccgcggg tggttggtgc cggggttctg gcccatcacg atgatgaggt cggccttgcc
     6241 gaagtcgtca taggagatcg tgctcttgcc gatgccgatc acctcgccca gcgccgtgcc
     6301 ggtggactca tggcacatgt tcgagcagtc ggggaggttg ttggttccga gggtgcgcgc
     6361 catcagctgg tagatgaagg cggtctcgtt ggcggtgcgg ccgctcgtgt agaaggtcgc
     6421 ctcgtcgggc gagtccatcg ccttcaggcg gtcggcgatg agcgtgaacg cctcatccca
     6481 ggagatcggc cggtagttgt cctggccctg gggcttgtag acgggttcga ccagacgacc
     6541 ctgcatgccc agccagtact ccgacttggt gagcaggctg ctgatggagt tctgcgccca
     6601 gaacgcggtg gggatcgtga cgggcgtggc ctcccaggtg acggccttcg cgccgttctc
     6661 gcagaactcg aacgtcttgc ggtggtcggg atcggcccac gcgcagctca tgcagtcgaa
     6721 gccgtccttc tggttcatgt ccgtgagcag ggtcagcgcg cgccgcacgc ccatctccct
     6781 gatgccgggc accatcgagt gcagcacccc gggcacgcct gcagcccact ccttcggcgg
     6841 gccgacctcc gcgtccctcg cgtcgtcgct gatgtcctgg atcggggcct tcgccgccat
     6901 cacgtgctcc ttcgcctggg tctctctctg gggtatcagc cgcatgccgc ccatgcaagg
     6961 gcctgaggtg cgttcgcggt ccggtcacac ggccgcgggc actgcctcgc gcacgcgctc
     7021 gggacgtgag tagacgttca tggtcgcgcc gcgcacgaag ccgacaaggg tgagcccggt
     7081 ggattcggcc atctcgacgg ccagcgacga cggcgccgat acggccgaca gcatcgggat
     7141 gcctgccatg accgccttct gcaccagttc gaagctcgcc cgtcccgaca cctgcagcac
     7201 ggtgccggtc agcggcaggc gtccgttcag caccgcccat ccgaccacct tgtcgacggc
     7261 gttgtgccgc ccgacgtcct ccctcaccac caggagctcg ccggtcgcgg agtcgtacag
     7321 gcccgccgcg tgcagcccgc ccgtcttctc gaagacctcc tggcgtgagc gcagcatgtt
     7381 cggcaggtcg gcgagggtcg cggcatccac caccagcgga tccgcggaga cgtcgaacgc
     7441 cgaggtggtc gcgacctgct cgatggacgc cgcaccgcac acgccgcagg cgctggtcgt
     7501 gtacgagtgc cgggcccgcg acggatcggg cagcggcgtg ccgggagcca gcgtgacgtc
     7561 caggacgttg taggtgttgc cgccgtcggc cgccgatgcg ccgagggcgt a
//
LOCUS       DATMSX010000011        12232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19131,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000011 DATMSX010000000
VERSION     DATMSX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19131"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..550
                     /gene="groES"
                     /locus_tag="VN041_00375"
     CDS             254..550
                     /gene="groES"
                     /locus_tag="VN041_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWU27534.1"
                     /translation="MSVSIKPLEDRIVIQQVEAEQTTASGLVIPDTAKEKPQEGQVVA
                     VGPGRIDDNGNRVPLDVAVGDRVLYSKYGGTEVKFGADEYLVLSARDVLAVVVR"
     gene            625..1539
                     /gene="rarD"
                     /locus_tag="VN041_00380"
     CDS             625..1539
                     /gene="rarD"
                     /locus_tag="VN041_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HWU27535.1"
                     /translation="MSTKSAGTLYTIGAYLLWGVFPLYFLLLDPTGPFEVVAWRLLLT
                     LVFCILLLAVTRGWEKLRIIWRQPKLLVLTALAGALIYINWQVFVIAAMSGHVVETSL
                     GYFINPIFTVLLGVLVLHERIRMAQWVAIGIAAVAVGVIIVAYGSFPWIALSLTASFG
                     LYGLVKKKIGPSVDAVSGLTLESFWMIPIAAVILVVVAQDGHGITMGTVSPLHTVLLS
                     CAGIATAVPLLLFAAGTRRVDLSLIGMIQFITPIMQFLLGWAVLREPMPAERWIGFIL
                     VWIAIAVFVVDLLVQKGRGRREAAAELV"
     gene            1658..2911
                     /locus_tag="VN041_00385"
     CDS             1658..2911
                     /locus_tag="VN041_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU27536.1"
                     /translation="MNALKGSRPARIFAGIALVSASALVISGCASTPASEKPTDGGKK
                     PAANVTLKMGSLLPSTGTLAFLDPPMEAGVQLAVKQVNDANLGVKIELNSADEGDLDN
                     KAYETSMAKLQSEGITTLVGAASSGVTKLIVDGNTSAGILAISPSNTGVAFAGLSPLY
                     FRTAPSDALQGEVLANTIAQDKHKSLGIIYQNDPYGQGLEESIKKVFESTGGKVVAEA
                     SYNQGDGQFNAQVSQIAAAKPDAVAVVSYDQFVTIAPLLKNAGIDTSSLYLVDGNLVD
                     YPDLPVDLTGSKGTKAGAELPESFIKELNDVWTAKGNGELKSQTYSAEAYDAVILMAL
                     GALQADSNKGKDIADQLVEISGGSGKGEKCKSFADCAKLINDGKKVDYDGFSGDVTFD
                     KDHDPQGAAMGVYEYEKGNTFKRIS"
     gene            complement(2982..3719)
                     /locus_tag="VN041_00390"
     CDS             complement(2982..3719)
                     /locus_tag="VN041_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27537.1"
                     /translation="MTDDVVVELTGVHAGYLPGVNILNGANLVARKGELIGIIGPNGA
                     GKSTLLKAIFGLVDVREGKITVNGESIVGLKADKLVARGVGFVPQTNNVFPSLTIEEN
                     LQMGLYQNPKAYAERLDFVTGIFAELGKRLKQRAGSLSGGERQMVAMSRALMMDPHVL
                     LLDEPSAGLSPVRQDDAFIRVSDINKAGVTTIMVEQNARRCLQICDRGYVLDQGRDAY
                     EGTGRDLLNDPKVIGLYLGTLGQEDAA"
     gene            complement(3716..4723)
                     /locus_tag="VN041_00395"
     CDS             complement(3716..4723)
                     /locus_tag="VN041_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27538.1"
                     /translation="MTGSIRRPKTTGLTKGPAAPGVPKVDPILIADAVQRRFGGLIAV
                     DVDHLEIPRGAITALIGPNGAGKTTLFNLLCGFDRPNTGTWSFDGKNLAGVPSFKVAR
                     MGQVRTFQLTKALSLLTVLENMKLGAPHQTGERFWQSLFPVLWRKQEKEIEDKARGLL
                     TRFKLDAKEKDFAAALSGGQRKLLEMARALMSDPSLVMLDEPMAGVNPALTQSLLDHI
                     LDLKDRGMTVLFVEHDMHMVRHIADWVVVMAEGRVVAEGPPEEVMEDQAVVDAYLGAH
                     QDVDLGVVTGRLSVVDGDAAAASEELAARIREKIEAEAEAELETDTDATSSTGTEEEK
                     S"
     gene            complement(4746..5723)
                     /locus_tag="VN041_00400"
     CDS             complement(4746..5723)
                     /locus_tag="VN041_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU27539.1"
                     /translation="MDFGSIFGNTLAYLLSPVTIAYALAATGLAVHFGYAGLLNFGMA
                     AFMAVGGYGYAISVLSFGLPWWLGMIIGLLGGALFALILGIPTLRLRADYLAIATIAT
                     AEIVRLLFLTELFEKWTNGAGGLSGYNGGFRDINPFPPGQYGFGPWTFNEVGLWNRVF
                     GLIMLAIAILLVWALMRSPWGRVLKGIREDEDAVRSLGKNVFAYKMQALVVGGVIGAL
                     GGIVFVMPSAVVPGSYSTSLTFFLWTVLLLGGAATVFGPTLGAVLFWMVFAFLGNLLP
                     QMSAAGMLPMSSTQADVVRYIIVGVVLMLIVIFRPQGILGDKREMTFVK"
     gene            complement(5726..7075)
                     /locus_tag="VN041_00405"
     CDS             complement(5726..7075)
                     /locus_tag="VN041_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU27540.1"
                     /translation="MGLRTMALRRPQNGLIALLATLLAAFLVLGIPAGSAQAATTPDG
                     QEITQFYFAGNITYKDKPIPDVKLSVKGNGFEGETKTDAEGSWRLYVPKKAEYTIAID
                     EDTLPKGVIVDAAQLPDGVKPVAGTSGSFEAEFGLTGTKIVNLFLGQGERVTVSFLDQ
                     LVNRTVGGINFGLLLGLASMGAALIYGTTRLSNFAHGEMVTWGGVAVVIVCSLLRLPL
                     WLGIAVAVIAGGLLGWAMDAGLWRPLRKRGLGVVQLMIVSIGLSLALRYALQYIIGGA
                     TEQLPGASPAPMTFGPISLSYIDLIGMGVSLVAILGMAYFLTRTRIGKATRAISDNPQ
                     LAAASGIDVDKVIRITWVLSGVLAAVSGILWAYFRPGVKWDMGMQMLLLMFCAITLGG
                     LGSAFGALIGSLIVGIAVEISTLWIPTDLKYASALIALIIVLLVRPQGLFGRRERLG"
     gene            7359..8864
                     /gene="guaB"
                     /locus_tag="VN041_00410"
     CDS             7359..8864
                     /gene="guaB"
                     /locus_tag="VN041_00410"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWU27541.1"
                     /translation="MPQHDPFGFVGLTYDDVLLLPGHTDVIPSEADTSSRITRRISVA
                     TPLLSSAMDTVTEARMAIAMAREGGIGILHRNLSIADQAADVDRVKRSESGMITDPIT
                     TTADATVDEVDQLCAKYRISGLPVVDDEGRLVGIITNRDMRFVSGFERRTTLVKDVMT
                     SENLVTAPVGVAAGEVIALFAQHRVEKLPLIDENGKLAGLITIKDFDKSEKYPLATKD
                     DQGRLRVGAAIGFFGDAWERAEALRDAGVDVLVVDTANGQSQGVIDLVKRLKADPTFE
                     HIDIIGGNVATREGAQALVDAGVDAVKVGVGPGSICTTRIVAGVGVPQVTAVYEASLA
                     AGPAGIPVIADGGLQYSGDIAKALVAGADAVMLGSLLAGTDESPGEIVFQSGKQFKLY
                     RGMGSLGAMQTRGKQTSYSKDRYFQADVPSDDKLIPEGIEGQVPYRGPVSAVAYQLIG
                     GLRQSMFYVGARTIEELKSRGKFVRITSAGLKESHPHDVQIVVEAPNYNKR"
     gene            8962..9237
                     /locus_tag="VN041_00415"
     CDS             8962..9237
                     /locus_tag="VN041_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWU27542.1"
                     /translation="MCRSIHTLHNFEPAATSEEVHAAALQYVRKIAGTTKPSKANQEA
                     FDHAVAEIAHITQHLLNDLVATRPPKNRDEEAAKARAKAVASGRYAA"
     gene            9318..9869
                     /locus_tag="VN041_00420"
     CDS             9318..9869
                     /locus_tag="VN041_00420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWU27543.1"
                     /translation="MTLLNDQFTAIADVTADERAQRLTDAGTTWNERIARDTSNAQLT
                     YKVTGRGVGSVGTEIRAGKHRFLVDEPAALAGDDAAASPVEYALGALISCQVVVFRLY
                     AQALGLTIDDIEVTAEGDLDVRKLFGIDESGRAGFHDVRVKVDITGPNTAEEYERLRA
                     VVEEHCPVLDLFANPVPTSGALV"
     gene            9980..11356
                     /locus_tag="VN041_00425"
     CDS             9980..11356
                     /locus_tag="VN041_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27544.1"
                     /translation="MGSRSNSLSRGQVTRYAIGSLGTGGFATLPGLVLVYYLTDSLGV
                     TALAAGAIVTAAKVWDVLIDPVIGGISDHALAHTGSRRRTMLIGGIGLPIAFILTFAV
                     PAGLGPVPAALWVLVAFMFAATFFSLFQVPYIALPAELTDDYRERTRLLSWRVVLLTV
                     AILLFGAGGPELRAMFPQDPLLGYLVMAIVAAAILGFGMLTSASVAPSRPLLPVRPGA
                     SIAEHYRDGIAVLRESQPFRALLVTFLLQGLATGMMLAAAQYVARWVLGDETAVTQLF
                     VALIAPALLMAPVWKLIADRIGKERAFRLASAVFVLATLVLGAMVWMPGWWILAPVAL
                     AGAAYAGMQTLPMAMLPDVIAYDRTLRAEEGQGSGGANRSGIFGGVWTAGETTGMALG
                     GTVLTIVLAITGYLETTVGKTVEQPESAVVGIAVSFSILPAILMVASMVTLARYHLRE
                     HDIVGTSA"
     gene            11353..>12232
                     /locus_tag="VN041_00430"
     CDS             11353..>12232
                     /locus_tag="VN041_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWU27545.1"
                     /translation="MTGGTTSDILERLRALRAGDAPTHGGRVLSYVYDSGLEELDELA
                     DAAARLARPLNGLDPTVFLSISAMERDVIAFTRRMLHGSRSVVGSVTSGGTESCLLAV
                     KTARDLWREAHPGGGRPRLLTASTVHAAFQKAAHLFDLDWEPVPAGIDGAVRAQDLID
                     RMDDDVALVVVSAPAYPSGALDPVSAVAGAAADRGLSCHVDSCFGGFVLPWWPDLPAW
                     DFRVRGVTSISADLHKFGYAPKGVSLLLHRGRRRHRAQFFATTRWAGYPVVNPTLLGS
                     RSASPLAAGWAITQHLG"
BASE COUNT         1995 a         4182 c         4101 g         1954 t
ORIGIN      
        1 cgcgcagccg cgacacggcg cgcgcgacgt cgccggacga ctcgaccggc ccgacctcgt
       61 cgagcagcgc gaggccgtcg cgggtgagca gggcggtcag ctcggacatc tccacccgac
      121 cagcctacgg cggcccgccc gattggcact cgcattgcat gagtgccagc cgtcggcata
      181 cactggagtt agcaccctca gcatgagagt gctaacagtc ttctgatctc agtcacgaaa
      241 gaagaggtac accgtgtcgg tttccatcaa gccgctcgag gaccgcatcg tcatccagca
      301 ggtcgaggca gagcagacca ccgcaagcgg tctggtcatc cccgacaccg ccaaggagaa
      361 gccccaggag ggccaggtcg tggctgtggg ccccggccgc atcgacgaca acggcaaccg
      421 cgtcccgctc gacgtcgccg ttggcgaccg cgtgctctac agcaagtacg gcggaaccga
      481 ggtcaagttc ggcgccgacg agtacctcgt gctctcggct cgcgacgtcc tcgcggtcgt
      541 cgtccgctga cgctgacgtt ctccagggcc cggatgcatg gcatccgggc ccttcgtcgt
      601 tccaggcgcc actaggcttg atcggtgagc acgaagagcg cggggactct gtacacgatc
      661 ggggcgtacc tgctctgggg cgtcttcccg ctgtacttcc tcctgctcga cccgaccggc
      721 ccgttcgagg tcgtggcatg gcggctgctg ctcacgctcg tgttctgcat cctgctgctg
      781 gccgtgaccc gcggctggga gaagctgcgc atcatctggc gtcagcccaa gctgctggtg
      841 ctcacagcgc tggccggtgc cctgatctac atcaactggc aggtcttcgt gatcgccgcg
      901 atgagcggcc acgtggtcga gacgagcctg ggctatttca tcaaccccat cttcacggta
      961 ctgctgggcg tgctggtgct gcacgagcgc atcaggatgg cccagtgggt cgcgatcggc
     1021 atcgccgcgg tcgcggtggg cgtgatcatc gtcgcctacg gctccttccc gtggatcgcc
     1081 ctgtcgctga cagcgtcctt cgggctttac gggctggtca agaagaagat cggtccttcc
     1141 gtcgacgcgg tcagcggcct cacgctggag tcgttctgga tgatcccgat cgccgcggtc
     1201 atcctcgtcg tcgtggcgca ggacggccac ggcatcacga tgggcaccgt cagcccgctg
     1261 cacaccgtgc tgctgtcctg cgcgggcatc gccaccgcgg tgccgctgct gcttttcgcc
     1321 gccggcacgc ggcgcgtcga tctgtcgctg atcggcatga tccagttcat caccccgatc
     1381 atgcagttcc tgctgggctg ggcagtgctg agggagccca tgcccgccga gcgctggatc
     1441 gggttcatcc tggtgtggat cgcgatcgcc gtgttcgtcg tcgacctgct ggtgcagaag
     1501 gggcgaggcc gccgcgaggc cgccgccgag ctggtctgag gaagccccgg gatcgttaag
     1561 ggaccgagac ccgcgcgagc tcggaccgca tccctgaccg catagggtaa aggcatccga
     1621 aagtggtact ccacgttcag ttacgcaagg gagcaacatg aacgcattga agggctcgcg
     1681 tccggcgagg atcttcgcag ggatcgcgct ggtcagcgca tccgcactgg tgatctccgg
     1741 ctgcgccagc acgccggcat ccgagaagcc gaccgacggt ggcaagaagc cggccgcgaa
     1801 cgtcaccctc aagatgggtt cgctgctgcc gtccaccgga acgctcgcgt tcctcgaccc
     1861 gcccatggag gctggcgtcc agctcgctgt gaagcaggtc aacgacgcga atctcggcgt
     1921 gaagatcgag ctgaactccg ccgacgaggg tgatctcgac aacaaggcgt acgagacctc
     1981 gatggcgaag ctgcagagcg aaggaatcac cacactcgtc ggcgcggcgt cttcgggcgt
     2041 caccaagctg atcgtggacg ggaacacgag cgccggcatc ctagcgatct cgccgtcgaa
     2101 cacgggtgtc gcgttcgcgg gcctgagccc gctgtacttc cgcacggcgc cgagcgacgc
     2161 gctgcagggc gaggtgctgg cgaacacgat cgcccaggac aagcacaagt cgctcgggat
     2221 catctaccag aacgacccgt acggccaggg gctggaggag tccatcaaga aggtgttcga
     2281 gagcaccggc ggcaaggtcg tcgccgaggc gtcgtacaac cagggcgacg gacagttcaa
     2341 cgcccaggtc agccagatcg cggccgccaa gccggatgcc gtggccgtcg tctcctacga
     2401 ccagttcgtc acgatcgcgc cgctgctgaa gaatgccggg atcgacacca gctcgctgta
     2461 cctcgtcgac ggcaacctgg tggactaccc cgacctgccg gtcgacctga ctggctcgaa
     2521 gggcaccaag gccggtgcag agctgccgga gagcttcatc aaggaactca atgacgtgtg
     2581 gacggccaag ggcaacggcg agctcaagtc gcagacctac tcggccgagg cgtatgacgc
     2641 agtcatcctg atggcactgg gggcgctgca ggcggactcg aacaagggca aggacatcgc
     2701 cgaccagctg gtcgagatct cgggcggctc gggcaagggc gagaagtgca agtccttcgc
     2761 cgactgcgcc aagctcatca acgacggcaa gaaggtcgac tacgacggct tctccggtga
     2821 cgtgaccttc gacaaggacc acgatccgca gggcgccgcg atgggcgtct acgagtacga
     2881 gaagggcaac accttcaagc gcatcagctg acgcgcggca gtatgaagag aggtcccgta
     2941 tgctcagcat ccgggacctc tcttctgtca gggcgaagac gtcaggcggc gtcctcctgg
     3001 cccagtgtgc ccaggtacag gccgatcacc ttcgggtcgt tcagcagatc gcgtccggtc
     3061 ccctcgtagg cgtcccggcc ctggtcgagc acgtagccgc ggtcgcagat ctgcaggcag
     3121 cggcgggcgt tctgctcgac catgatggtt gtcacgccgg ccttgttgat gtcggagacg
     3181 cggatgaacg catcgtcttg ccgcacgggg gacaggcccg cggaaggctc gtccagcagc
     3241 agcacgtgag ggtccatcat cagggcgcgg gacatcgcca ccatctggcg ctcgccgccg
     3301 gagagggagc cggcgcgctg cttgaggcgc ttgcccagct cggcgaagat gcccgtgacg
     3361 aagtcgaggc gctcggcgta cgccttcggg ttctggtaca gccccatctg caggttctcc
     3421 tcgatcgtga gggaggggaa cacgttgttg gtctgcggca cgaagcccac gccgcgcgcg
     3481 acgagcttgt cggctttcag gcccacgatg ctctcgccgt tcacggtgat cttgccctcg
     3541 cgcacgtcca ccaggccgaa gatcgccttc agcagcgtgg acttgccggc cccgttcgga
     3601 ccgatgatcc cgatcagctc gcccttgcgt gccaccaggt tcgctccgtt gaggatgttc
     3661 acgcccggca ggtagccggc atggactccg gtgagctcca ccaccacgtc gtcggtcatg
     3721 acttctcctc ctcggtgccg gtgctactgg tggcatccgt atcggtttcc agttcggcct
     3781 ccgcttcggc ctcgatcttc tctcggatgc gggctgcgag ctcctccgag gcggcggcgg
     3841 catctccgtc gacgacggag agccgtcccg tcaccacgcc caggtcgacg tcctgatgcg
     3901 caccgaggta cgcgtctaca acggcctggt cctccatgac ctcctcgggt gggccctcag
     3961 ccaccacacg gccctcggcc atcacgacga cccagtccgc gatgtgccgc accatgtgca
     4021 tgtcgtgctc gacgaagagc acggtcatgc cgcggtcctt gagatcgagg atgtggtcca
     4081 gcagcgactg cgtgagcgcc gggttgacgc cggccatcgg ctcgtcgagc atcaccaggc
     4141 tcgggtcgct catcagagcc cgcgccatct ccagcagctt gcgctgcccg cctgagagcg
     4201 ctgccgcgaa gtccttctcc ttggcgtcga gcttgaagcg cgtgagaagg ccgcgggcct
     4261 tgtcctcgat ctccttctcc tgcttgcgcc agagcaccgg gaacaggctc tgccagaacc
     4321 tctcgccggt ctggtgcggg gcaccaagtt tcatgttctc cagcaccgtc agcagcgaca
     4381 gcgccttggt cagctggaaa gtgcgaacct gtcccatcct ggcaaccttg aacgagggga
     4441 cgccggcgag gttcttcccg tcgaatgacc aggttccggt gttgggcctg tcgaagccgc
     4501 acagcaggtt gaacagcgtc gtcttgccgg cgccgttcgg gccgatgagc gcagtgatgg
     4561 ccccgcgggg gatctcgagg tgatccacat cgaccgcgat caggccgccg aagcgccgct
     4621 gcacggcatc cgcgatgagg atcggatcga ccttagggac gcccggtgcg gccggtccct
     4681 tcgtgaggcc ggtggtcttg gggcgacgga tgctgccggt caccggcgcc gcctcgggag
     4741 tgttctcact tgacaaaggt catctccctc ttgtctccga ggatgccctg ggggcggaag
     4801 atcacgatga gcatcagcac gacgcccacg atgatgtagc gcacgacatc ggcctgggtg
     4861 ctgctcatgg gcagcatgcc tgccgccgac atctgcggga gcaggttgcc gaggaaggcg
     4921 aagaccatcc agaacagcac tgcgccgagc gtcgggccga atacggtcgc ggcgccgccg
     4981 agcagcagga ccgtccacag gaagaacgtc agcgatgtcg agtagctgcc cggcacgacg
     5041 gcgctgggca tgacgaagac gatgccgccg agagcgccga tcacgccgcc gaccacgagc
     5101 gcctgcatct tgtaagcgaa tacgttctta ccgagggagc gcacggcgtc ctcatcctcg
     5161 cggatgccct tgagcacgcg gccccacggg ctgcgcatca gcgcccacac cagcaggatc
     5221 gcgatggcca gcatgatcag gccgaacacg cggttccaca gcccgacctc gttgaacgtc
     5281 cacggaccga agccgtactg gccagggggg aacgggttga tgtctcggaa cccgccgttg
     5341 tagccactga ggccgccggc gccattggtc cacttctcga acagctccgt gaggaacagc
     5401 agacgcacga tctccgccgt cgcgatcgtc gcgatcgcga ggtagtccgc tcgcagccgc
     5461 agggtgggga taccgaggat gagcgcgaag agcgcgccgc cgagcagtcc gatgatcatg
     5521 cccagccacc agggcaggcc gaagctgagc accgagatcg catagccgta tccgcctacg
     5581 gccatgaagg ccgccatgcc gaagttgagc agaccggcgt agccgaagtg cacggccagg
     5641 cccgtcgcgg ccagggcgta tgcgatggtg acgggactga gaaggtaggc gagagtgttc
     5701 ccgaagatgc ttccgaagtc catggtcagc ccagcctttc tctgcgaccg aacaggccct
     5761 gcgggcgcac cagcaggacg atgatgagcg cgatcagcgc gctggcgtac ttgaggtcgg
     5821 tgggaatcca cagggtcgag atctcgacgg cgatgccgac gatcagcgat ccgatcagtg
     5881 cgccgaaggc cgacccgagg ccgccgagcg tgatcgcgca gaacatcagc agcagcatct
     5941 gcatgcccat gtcccacttc acgccgggcc ggaagtacgc ccacaggatg ccggagacgg
     6001 cagcgagcac gccggagagg acccaggtga tccggatgac tttgtcgacg tcgatgccgg
     6061 atgcggcggc gagctgcggg ttgtcggaga tcgccctggt ggccttgccg atgcgcgtgc
     6121 gggtgaggaa gtaggccatg ccgaggatcg ccacgagact gacccccatc ccgatcagat
     6181 cgatgtacga cagcgagatc ggaccgaagg tcatcggtgc cgggctcgcg cccggcagct
     6241 gctcagtggc gccgccgatg atgtactgca gcgcgtagcg cagggcgagg gagaggccga
     6301 tgctcacgat catgagctgc accacgccga gtccccgctt gcgcaggggc ctccagagtc
     6361 cggcatccat cgcccagccg agcaggcctc cggcgatcac cgcgacggcg atcccgagcc
     6421 agagcggcag ccgcaggagc gagcacacga tcaccacggc cacgccgccc caggtgacca
     6481 tctcgccgtg cgcgaagttc gagagcctcg tcgtgccgta gatcagggcg gcgcccatcg
     6541 aggcgagtcc cagcagcaga ccgaagttga tgccgccgac cgtgcggttc accaattgat
     6601 ccaggaacga gaccgtgacc cgttcgccct ggccgaggaa gaggttgacg atcttggtgc
     6661 cggtcagacc gaactccgct tcgaacgacc cgctcgtccc cgcgacgggc ttcacaccgt
     6721 ctggcaactg cgcggcatcg acgatcacgc ccttgggcag cgtgtcctcg tcgattgcga
     6781 tcgtgtactc ggccttcttc ggcacgtaca ggcgccagga accctcggca tccgtcttgg
     6841 tctcgccttc gaagccgttc cccttgaccg agagcttcac gtcgggaatc ggcttgtcct
     6901 tgtacgtgat gttcccggcg aagtagaact gggtgatctc ctggccgtcg ggtgtcgtgg
     6961 cggcctgtgc tgaaccggcg ggtatgccga gcacgaggaa ggcggccaga agcgtcgcaa
     7021 gcaacgcgat cagcccgttc tgggggcgcc gaagcgccat ggttctgagt cccacgcatc
     7081 ctccacatcg agtgctgaag aaccgacacc gggtagtggt cggttctgga cgtcgcactg
     7141 agcgtatccc gctccaggcc gcatccgcac aaccgtgtcc ggatcggaac gaagcccggg
     7201 agtgtcgcac ggccgccgcc tgacagggct ctcggaatat ccgcaggcgt cagacgttta
     7261 gaatcggtac acgacggaca ccgctcccgt cccggctgca gtcgtcccgc gcactctcga
     7321 caatcgatcg cgcccccggc gcagaggagc cagttttcat gccccagcac gacccgttcg
     7381 gtttcgtcgg actcacctac gatgacgtgc tgcttctgcc cgggcacacg gacgtcatcc
     7441 ccagcgaagc ggacacctcc tcccgcatca cgcggcgcat ctcggtcgcg accccgctgc
     7501 tctccagcgc gatggacacg gtcaccgagg cgcgcatggc gatcgccatg gcgcgtgagg
     7561 gcggcatcgg catcctgcac cgcaacctct ccatcgccga ccaggccgcc gacgtcgacc
     7621 gcgtcaagcg cagcgagtcg ggcatgatca ccgacccgat caccacgacc gccgacgcca
     7681 ccgtcgacga ggtcgaccag ctgtgcgcca agtaccgcat ctcgggcttg ccggtcgtcg
     7741 acgacgaggg tcgcctggtg ggcatcatca ccaaccgcga catgcgattc gtctcgggct
     7801 tcgagcgcag gaccaccctg gtcaaggacg tcatgaccag cgagaacctg gtcaccgctc
     7861 ccgtcggcgt cgccgccggc gaggtcatcg ctctgttcgc tcagcaccgc gtcgagaagc
     7921 tgccgctcat cgatgagaac ggcaagctcg ccgggctgat caccatcaag gacttcgaca
     7981 agagcgagaa gtaccccctc gccaccaagg acgaccaggg ccgcctgcgc gtcggcgccg
     8041 cgatcggctt cttcggcgac gcctgggagc gcgccgaggc gctgcgcgac gcgggtgtgg
     8101 acgtgctggt cgtcgacacc gccaacggcc agtcgcaggg cgtgatcgac ctggtcaagc
     8161 gtctgaaggc cgacccgacc ttcgagcaca tcgacatcat cggcggcaac gtcgcgaccc
     8221 gcgagggcgc acaggcgctg gtggacgccg gcgtcgacgc cgtcaaggtg ggcgtgggcc
     8281 cgggctcgat ctgcaccacc cgcatcgtcg ccggagtcgg cgtgccgcag gtgaccgccg
     8341 tgtacgaggc ctcgctggcc gcaggtccgg caggcatccc ggtgatcgcc gacggcggcc
     8401 tgcagtactc gggtgacatc gccaaggcgc tggtcgccgg ggcggacgcc gtcatgctcg
     8461 gctccctgct cgccggcacc gacgagtcgc cgggcgagat cgtcttccag tcgggcaagc
     8521 agttcaagct gtaccgcggc atgggctcgc tcggcgcgat gcagacccgc ggcaagcaga
     8581 cctcgtactc gaaggaccgc tacttccagg ccgacgtccc cagtgacgac aagctcatcc
     8641 ccgagggcat cgagggccag gtgccctacc gcggcccggt ctcggcggtg gcctaccagc
     8701 tgatcggcgg cctgcgccag tcgatgttct acgtcggcgc gcgcaccatc gaggagctca
     8761 agtcgcgcgg aaagttcgtg cgcatcacct cggcgggcct gaaggagtcg cacccgcacg
     8821 acgtgcagat cgtcgtcgag gcgccgaact acaacaagcg ctgagagtgc cctgaagagg
     8881 gcggatgccg cggcatccgc cctcttcgca tgtccgcgag ggggggttcc ggatgtcgga
     8941 cgctccgcgt agcgtgccgg tatgtgccgc agcatccaca cccttcacaa cttcgagccc
     9001 gccgccacct ctgaggaggt gcacgcggca gccctgcagt acgtgcgcaa gatcgccggg
     9061 acgaccaagc cgtccaaggc caaccaggag gcgttcgatc acgccgtcgc cgagatcgcg
     9121 cacatcactc agcacctgct gaacgacctg gtcgccacac gtccgccgaa gaaccgcgac
     9181 gaggaggcgg cgaaggcgcg tgccaaagcc gtcgcgtcgg gacgctacgc ggcctgagca
     9241 ttgcgccgtg tgacgcggcg ttgcggggtg tgacggcacg cgagcgcggc atccggccgc
     9301 cgggctaccg tcgccgcatg acacttctca acgaccagtt caccgccatc gcagacgtca
     9361 ccgctgacga gcgcgcccag cgcctcaccg acgccgggac gacctggaac gagcgcatcg
     9421 cccgcgacac ctccaacgcg cagctgacct ataaggtcac tggtcgcgga gtcggttccg
     9481 tcggcacgga gatccgggcg ggcaagcacc gcttcctcgt cgacgagccc gcggcactcg
     9541 ccggtgatga cgcggccgcc agccccgtcg agtacgcgct cggcgcgctg atctcctgtc
     9601 aggtggtggt gtttcgcctc tacgcgcagg ccctcggcct caccatcgac gacatcgagg
     9661 tcaccgccga gggcgacctc gacgtgcgca agctgttcgg catcgacgag tcgggccgcg
     9721 ccggcttcca tgacgtgcgc gtgaaggtcg acatcacggg cccgaacacg gccgaggagt
     9781 acgagaggct ccgcgccgtc gtcgaggagc actgcccggt gctggacctg ttcgcgaacc
     9841 ccgtcccgac ctcgggcgcg ctggtctgag gcatcccgct cgccgtcgcc tcgcgggtcg
     9901 cgacaggccg cttccgcccg tctgaggcgg cggtccgcga cccgcgagcc cgggccggct
     9961 caccactaga gtgagcggca tgggttcacg gtcgaattcg ctcagccgcg ggcaggtcac
    10021 ccgctacgcg atcggttcgc tcggcacggg cggtttcgcg acgctccccg ggctggtcct
    10081 cgtctattac ctgaccgact cgctgggcgt caccgcgctc gccgccggcg caatcgtgac
    10141 cgccgcgaag gtgtgggacg tgctgatcga tcccgtcatc ggcggcatca gcgatcacgc
    10201 cctcgcccac accggaagcc gccgccgcac catgctcatc ggaggcatcg ggctgccgat
    10261 cgccttcatc ctgaccttcg cggtgcccgc cgggctcgga ccggtgcccg cggcgctgtg
    10321 ggtgctggtg gccttcatgt tcgccgccac cttcttcagc ctgttccagg tgccgtacat
    10381 cgcgctgccc gcagagctca ccgacgacta ccgcgagcgc acccggctgc tcagctggcg
    10441 cgtggtgctg ctgaccgtcg ccatcctgct gttcggagcc ggcggtccgg agctgcgggc
    10501 gatgttcccg caggatccgc tgctgggcta cttggtgatg gcgatcgtcg ccgccgcgat
    10561 cctcggattc gggatgctga cctccgcctc ggtcgctccg agccggccgc tgctgccggt
    10621 gcgaccgggc gcgagcatcg ccgagcacta ccgcgacggc atcgcggtgc tccgcgagag
    10681 ccagccgttc cgggcgctgc tggtgacgtt cctgctgcag ggactggcga cggggatgat
    10741 gctcgcggcc gcgcagtacg tggcccgctg ggttctcggc gacgagacgg cggtgaccca
    10801 gctgttcgtg gcactgatcg cccccgccct tctgatggcg ccggtgtgga agctgatcgc
    10861 cgaccgcatc ggcaaggagc gcgccttccg gctggccagc gcggtgttcg tgctggcgac
    10921 gctcgtgctg ggcgcgatgg tgtggatgcc gggctggtgg atcctcgcgc ccgtcgccct
    10981 cgcgggcgcc gcctatgcgg gcatgcagac gctgccgatg gccatgctgc ccgacgtgat
    11041 cgcctacgac cgcacgctgc gtgcggagga ggggcagggg agcgggggtg cgaaccgctc
    11101 cggcatcttc ggcggcgtgt ggaccgcggg ggagaccacc ggcatggctc tgggcggcac
    11161 ggtgctcacg atcgtgctgg ccatcaccgg ctacctggag accacggtcg gtaagacggt
    11221 cgagcagccc gagtcggccg tggttggcat cgcggtgtcg ttcagcatcc tgccggcgat
    11281 cctgatggtc gcgagcatgg tgaccctcgc ccggtaccac ctgcgtgagc acgacatcgt
    11341 gggcacgtcg gcatgaccgg cggcaccacg agcgacatcc tggaacgcct ccgcgccctg
    11401 cgcgcggggg atgccccgac gcacggcggt cgtgtgctgt cctacgtcta cgactcgggt
    11461 ctcgaggagc tcgacgagct cgcggacgcg gcggcgcgac tcgcccgccc gctgaacgga
    11521 ctcgacccga ccgtcttcct ctccatctcg gcgatggagc gcgacgtcat cgccttcacc
    11581 cggcggatgc tgcacggcag tcgctccgtg gtgggatcgg tcacgagcgg cgggacggag
    11641 agctgcctgc tcgcggtgaa gaccgcgcgc gacctgtggc gcgaagcgca tccgggcggc
    11701 ggtcgcccga ggctgctcac ggcgtcgacc gtgcacgccg ccttccagaa ggccgcgcac
    11761 ctgttcgacc tggactggga gccggtgccg gccgggatcg acggcgccgt gcgtgcgcag
    11821 gatctcatcg accggatgga tgacgatgtc gcgctggtcg tcgtcagcgc accggcgtac
    11881 ccgagcggcg cgctggaccc ggtgtcggcg gtcgcgggcg ccgcggcgga tcgcgggctc
    11941 tcctgtcacg tcgactcctg cttcggaggg ttcgtgctgc cctggtggcc cgatctgccc
    12001 gcgtgggact tccgggtgcg cggcgtcacc agcatctccg ccgacctgca caagttcggc
    12061 tacgcgccca agggcgtctc gttgctgctg catcgggggc gacgccggca ccgggcgcag
    12121 ttcttcgcga ccacgcgctg ggccgggtac ccggtggtga atccgaccct gctcggctcg
    12181 cggtcggcgt ccccgctggc cgcaggctgg gcgatcacgc agcacctggg gt
//
LOCUS       DATMSX010000012         6876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123427,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000012 DATMSX010000000
VERSION     DATMSX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123427"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VN041_00435"
     CDS             complement(<1..312)
                     /locus_tag="VN041_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWU27546.1"
                     /translation="MASDPDDDPRIGDISEGTVLRAILARTSTASHAILGPGDDAAVI
                     SAPSASVVATTDTLVDGPDFRTAWSSGYDLGWKAAAVNLADVAAMGATPTALLVALAV
                     PR"
     gene            336..809
                     /locus_tag="VN041_00440"
     CDS             336..809
                     /locus_tag="VN041_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HWU27547.1"
                     /translation="MLRRLVPVAGVLTLLLLTACSSTVALKPEPDSNNPACAEVTVRL
                     PDAVGELSRRWTDAQATAAYGDPTAVIVACGVDVPGPTATLQCVTLDGIDWLVDDSQA
                     PKMRMTTYGRNPAVQVFVDTKLVGANEVLSNTGLVSGIRTIKATSQCTAPAELPK"
     gene            complement(813..1901)
                     /locus_tag="VN041_00445"
     CDS             complement(813..1901)
                     /locus_tag="VN041_00445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWU27548.1"
                     /translation="MDRQTVVVLFGGRSSEHSISSATAGGVLAAIDRDRYAVIPVGIT
                     REGAFVLEQDDPAKFALDAAKMPEVVDNGTRMRWPEPGGDRTLRVVAPDGSTVDLGEI
                     DVVLPLLHGLHGEDGAIQGFFDVLEVPYAGGGILDSAVCLDKHFTKLALSAAGIAVAP
                     GITVRSAEWDADPESVRERVRALGETVFVKPASAGSSVGVSRVDGGAGLDEAFRIAFD
                     EDEKVLVETRVTGREIEVGVLAGRDGERARASLPGEVVLTSRSFYDFEGKYLGGEGVE
                     IVCPAQVDDELIGRLQETAVRAFEAVDGKGLARVDFFVTSDGQLVINELNTMPGFTPF
                     SMFPKCWMASGMTYGELITELIEAGLRK"
     gene            complement(1801..2943)
                     /locus_tag="VN041_00450"
     CDS             complement(1801..2943)
                     /locus_tag="VN041_00450"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWU27549.1"
                     /translation="MTPRREPSGTGTRTAIVGAGSWGTTFGKILADGGAQVTMWARRP
                     ELAQEIDEAKRNSRYLPGINLPRGMRATADIERALDGVQQVYLSVPSQTLRENLRAFR
                     PLLAAGDFPVVSLMKGVERGTGLRMSQVIEQELDIDPMRIAVASGPNLALEIAREQPT
                     AAVISSRSQETADAVARTARNRYFRSFVNTDVIGTEFGGVLKNLIAVAIGIVDGVGYG
                     ENTKASIITRGLVEMTDFAVANGAQPETLQGLAGLGDLIATCQSPLSRNNTAGRLLGQ
                     GYGIQDVIKQMNQTAEGLASVAPILQLAHEADVYMPIVEQVKMVLDGKMNPRDIAPHL
                     TTDDDTPQEERTNYGQADGGGALRRAFQRAFDQFRNGGRGAGGDRP"
     gene            complement(2933..3682)
                     /locus_tag="VN041_00455"
     CDS             complement(2933..3682)
                     /locus_tag="VN041_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU27550.1"
                     /translation="MAASERSRPSLFWVLAVLVIPPMSLLAKIRVTGAEKLPRQGAFV
                     LAPNHYSELDPLIVAMSVYRLGRLPRFMAKESLFRVPVVGWLLKRTGMIPVSRSASAT
                     AAKQTLKQSAELVEHGRGVIVYPEGTLTRDPDLWPMRGKSGAVRLALSGDLPLIPMAQ
                     WGTQEIMGRYQKGLSLWPLRKPVNVLIGDPVDLSDLRGRASEPAALNEATARLMAAIT
                     ALLEQLRDEKAPATRWNPTDHGQKETGRLDS"
     gene            complement(3687..5051)
                     /gene="murA"
                     /locus_tag="VN041_00460"
     CDS             complement(3687..5051)
                     /gene="murA"
                     /locus_tag="VN041_00460"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWU27551.1"
                     /translation="MTTPLRDAAVSEAPVPAGSVLAIRGGRPLRGRVDVKGAKNLATK
                     AMVAALLGETTSILRDVPDLSDVAVVRSLLEVHGVRVTEGDEPGSLVLDPSLVESAHY
                     EEIDAHAGASRIPILFCGPLLHRLGQAFIPDLGGCRIGDRPIDFHLDALRKFGAIVEK
                     QPSGIRLSAPNGLKGANIHLPYPSVGATEQVLLTAVRAQGQTELRNAAIEPEIMDLIA
                     VLQKMGAIISYEPNRVILIEGVETLRGYDHRAIFDRNEAASWACAALATDGEIFVAGA
                     RQQEMLTFLNVFRKAGGWFDVQEDGILFRREGALKPVVVETDVHPGFMTDWQQPLIVA
                     LTQAQGRSIVHETVYENRFGFTQALVKMGADIVVHPHGLQDGPRRVPRRELEQAAVIT
                     GPTPLHGTDIVVPDLRGGYSHVIAALTAEGESVVSGVDILSRGYEKFLDKLQALGADF
                     DVVR"
     gene            complement(5161..5757)
                     /gene="leuD"
                     /locus_tag="VN041_00465"
     CDS             complement(5161..5757)
                     /gene="leuD"
                     /locus_tag="VN041_00465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWU27552.1"
                     /translation="MEKFTTHTGVAAPLKRSNVDTDQIIPAVFLKRVTKTGFDDALFH
                     EWRKDPEFVLNQGPFQKASVLVAGPDFGTGSSREHAVWALRDYGFRVVLSPRFADIFR
                     GNSGKQGLLAATVDEADIERIWALIDEEPGREITVDLEARTATIGDFQAAIGIDDYTR
                     WRLLEGLDDIGLTLRNEDKIAQFEARRESWRPRTLPVR"
     gene            complement(5763..>6876)
                     /gene="leuC"
                     /locus_tag="VN041_00470"
     CDS             complement(5763..>6876)
                     /gene="leuC"
                     /locus_tag="VN041_00470"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWU27553.1"
                     /translation="FPFLSKRQGIVHVIGPESGATLPGMTVVCGDSHTSTHGAFGALA
                     HGIGTSEVEHVMATQTLPLKPFKTMAINVEGELRPGVTAKDIILAVIAKIGTGGGQGY
                     VLEYRGSAIRALSMEGRMTICNMSIEAGARAGMVAPDETTFAYVKDKPHAPQGQDWED
                     AVAYWRTLPSDPDAVFDAEVDIDASTLEPFVTWGTNPGQGSSLSASVPDPAEITDANE
                     RAAAERALQYMDLTPGTPLKDVKVDAVFMGSCTNSRIEDLRAFASVIEGKKKADGVRV
                     MVVPGSARVRLEAEAEGIDKIVEAFGAEWRFAGCSMCLGMNPDQLAPGERCASTSNRN
                     FEGRQGKGGRTHLVSPLVAAATAIRGTLSSPSDLEA"
BASE COUNT          995 a         2402 c         2388 g         1091 t
ORIGIN      
        1 gcggggcacc gccagcgcga ccagcagcgc cgtgggagtc gcacccatcg ccgccacatc
       61 ggccaggttc acggccgcgg ccttccagcc caggtcgtac ccgctcgacc aggccgtgcg
      121 gaagtccggg ccgtccacca gcgtgtcggt ggtcgcgacg acggacgccg agggcgccga
      181 gatcaccgcg gcgtcgtcgc ccggcccgag gatcgcgtgc gatgcggtcg acgtgcgcgc
      241 gaggatcgct cgcagcaccg tcccctccga gatgtcgccg atgcgcgggt cgtcgtcggg
      301 atcggatgcc atgccgtcaa cggtagcctg gaaacatgct tcgtcgtctc gtcccggtcg
      361 ccggagtgct gaccctcctc ctgctcaccg cctgctcgag cacggtcgcg ctcaagcccg
      421 aacccgactc gaacaacccc gcctgcgcgg aggtgacggt gcgcctcccc gacgcggtcg
      481 gcgagctcag caggcgctgg acggatgccc aggcgacggc cgcctacggc gacccgacgg
      541 ccgtgatcgt cgcgtgcggc gtggacgtcc ccgggccgac cgccacgctg cagtgcgtga
      601 ctctggacgg catcgactgg ctcgtcgacg actcccaggc gccgaagatg cgcatgacca
      661 cctacgggcg gaaccccgcc gtccaggtgt tcgtcgacac gaagctggtg ggcgccaacg
      721 aagtgctctc caacaccggt ctggtcagcg gcatccgcac gatcaaggcg accagccagt
      781 gcaccgcacc ggccgagctt ccgaagtagt ccctacttcc ggagccctgc ctcgatcagc
      841 tcggtgatca gctcgccgta ggtcatgccg gatgccatcc agcacttcgg gaacatggag
      901 aacggcgtga agccgggcat ggtgttcagc tcgttgatca cgagctgccc gtcggaggtc
      961 acgaagaagt cgacgcgggc cagtcccttg ccgtccaccg cctcgaacgc gcgcaccgcg
     1021 gtctcctgca ggcgtccgat cagctcgtcg tcgacctgcg cggggcagac gatctccacg
     1081 ccctcgccgc cgaggtactt gccctcgaag tcgtagaagc tgcgggaggt cagcacgacc
     1141 tcgccgggca gcgatgcccg cgcccgctcg ccgtcgcgtc cggcaaggac gccgacctcg
     1201 atctctcggc cggtcacgcg cgtctcgacg agcaccttct cgtcctcgtc gaacgcgatc
     1261 cggaacgcct cgtccagtcc ggcgccgccg tccacgcggg agactccgac gctcgatccg
     1321 gcactggctg gcttgacgaa cacggtctcg cccagcgcgc gcacacgctc gcgcacgctc
     1381 tcgggatcgg catcccactc cgcgctgcgc acggtgatgc cgggcgccac ggcgatgccc
     1441 gccgccgaca gcgcgagctt ggtgaagtgc ttgtccaggc acaccgcgga atcgaggatg
     1501 ccgccgccgg cgtagggcac ctccagcacg tcgaagaagc cctgtatggc gccgtcctcg
     1561 ccgtgcagac cgtgcaggag cggcagcacc acgtcgatct caccgaggtc gacggtcgac
     1621 ccgtcggggg cgacgacgcg cagcgtgcgg tccccgcccg gctccggcca ccgcatccgg
     1681 gtgccgttgt cgacgacctc gggcatcttc gcggcgtcca gcgcgaactt cgccgggtcg
     1741 tcctgctcca ggacgaacgc gccttcacgg gtgatcccga cggggatcac cgcgtagcgg
     1801 tcacggtcga tcgccgccag caccccgccc gccgttgcgg aactgatcga atgctcgctg
     1861 gaacgcccgc cgaagagcac caccaccgtc tgcctgtcca tagttggtcc tctcctcctg
     1921 gggagtgtcg tcgtcggtgg tcaggtgcgg ggcgatgtcg cgcgggttca tcttcccgtc
     1981 gagcaccatc ttcacctgct ccacgatggg catgtacacg tcggcctcgt gcgcgagctg
     2041 caggatcgga gcgacggatg ccagaccctc ggcggtctgg ttcatctgct tgatcacgtc
     2101 ctggatgccg tatccctggc ccagcaggcg gcccgccgtg ttgttgcggc tgagcggcga
     2161 ctggcaggtg gcgatcaggt cgccgaggcc agccaatccc tggagtgtct cgggctgcgc
     2221 gccgttggcg accgcgaagt cggtcatctc cacgagtccg cgcgtgatga tcgaggcctt
     2281 ggtgttctcg ccgtagccga cgccgtcgac gatgccgatc gccacggcga tgaggttctt
     2341 cagcacgccg ccgaactccg tgccgatcac gtcggtgttc acgaaggagc ggaagtagcg
     2401 gttccgggcg gtgcgggcca cggcatccgc cgtctcctgg ctgcgcgagg agatgacggc
     2461 cgccgtcggc tgctcgcgcg cgatctccag cgccaggttc ggtccggatg ccacggcgat
     2521 gcgcatcgga tcgatgtcga gctcctgctc gatgacctgg ctcatccgca ggcccgtgcc
     2581 gcgctcgacg cccttcatca ggctgaccac cgggaagtcg ccggcggcga gcagcggacg
     2641 gaaggcacgc aggttctcgc gcagcgtctg gctgggcacc gacaggtaca cctgctgcac
     2701 gccgtcgagc gcacgctcga tgtcggcggt cgcacgcatg ccgcgcggca ggttgatgcc
     2761 gggcaggtac cgggagttgc gcttcgcctc gtcgatctcc tgcgcgagtt cggggcgacg
     2821 cgcccacatg gtcacctggg cgcccccatc ggcgaggatc ttgccgaagg tggttcccca
     2881 gctgcccgca ccgacgatcg ccgtgcgggt tcccgtgccg gacggctctc gtctaggagt
     2941 caagacgccc cgtctccttc tgtccgtggt cggtggggtt ccagcgcgtg gccggtgcct
     3001 tctcgtcgcg gagctgctcg aggagcgcgg tgatggcggc catcaggcgc gcggtcgcct
     3061 cgttcagcgc cgcgggctcg gatgcccggc cccgcagatc ggagagatcg accggatcgc
     3121 cgatcagcac gttcaccggc ttgcgcagcg gccagaggct gaggcccttc tggtagcggc
     3181 ccatgatctc ctgcgttccc cattgcgcca tcgggatgag cgggaggtct ccggagagcg
     3241 ccaggcgcac cgcgccggac ttcccgcgca tcggccacag gtcggggtca cgggtcagcg
     3301 tgccctccgg gtagacgatg acgccgcggc cgtgctcgac cagctcggcg gactgcttga
     3361 gagtctgctt cgccgcggtg gccgatgcgc tgcgcgacac cgggatcatg ccggtgcgct
     3421 tgagcagcca gccgaccacg ggcacccgga acaggctctc cttcgccatg aagcgcggca
     3481 gccgtccgag acggtacacg gacatggcca cgatcagcgg gtccagctcc gagtagtggt
     3541 tcggggcgag cacgaaggcg ccctggcggg gcagcttctc cgccccggtg acgcggatct
     3601 tcgccagcag ggacatcggc gggatgacga gcaccgcgag cacccagaag aggctcgggc
     3661 ggctccgctc ggaagcagcc atcagctcac cggacgacgt cgaagtcggc gcccagcgcc
     3721 tgcagcttgt cgaggaactt ctcgtagccg cggctgagga tgtcgacacc ggagacgacg
     3781 gactcgccct cggcggtcaa cgccgcgatc acgtggctgt acccgccgcg caggtcgggc
     3841 accacgatgt cggtgccgtg cagcggcgtg ggacccgtga tgacggcggc ctgctccagc
     3901 tcgcggcgcg gcacgcgacg cggaccgtcc tgcaggccgt gcgggtgcac gacgatgtcg
     3961 gcgcccatct tgaccagggc ctgggtgaac ccgaaccggt tctcgtacac ggtctcgtgc
     4021 acgatcgagc ggccctgcgc ctgggtgagc gcgacgatca gcggctgctg ccagtcggtc
     4081 atgaaaccgg ggtgcacatc ggtctcgacg acgacgggct tgagcgcgcc ctcgcggcgg
     4141 aagaggatgc cgtcctcctg gacgtcgaac cagccgcccg ccttgcggaa cacgttcagg
     4201 aacgtcagca tctcctgctg acgggctccg gcgacgaaga tctcgccgtc ggtggccagc
     4261 gcggcgcacg cccaggacgc ggcctcgttg cggtcgaaga tcgcacggtg gtcgtagccc
     4321 cgcagcgtct ccacgccctc gatgaggatg acgcggttcg gctcgtagga gatgatcgcg
     4381 cccatcttct gcagcacggc gatcaggtcc atgatctccg gctcgatggc cgcgttgcgc
     4441 agctcggtct gcccctgtgc gcgcacggcc gtcagcagaa cctgctcggt ggcgccgacg
     4501 ctcgggtacg ggaggtggat gttcgcgccc ttgagcccgt tgggggccga gaggcggatg
     4561 ccgctgggct gcttctcgac gatcgcgccg aacttgcgca gcgcgtcgag gtggaagtcg
     4621 atcggacggt cgccgatgcg gcatccgccc aagtcgggga tgaacgcctg accgagacgg
     4681 tgcagcagcg ggccgcagaa caggatcggg atgcgggagg cgccggcgtg cgcgtcgatc
     4741 tcctcgtagt gggcggactc gaccaggctc gggtccagca ccagcgaacc gggctcgtcg
     4801 ccctcggtca cgcggacgcc gtgcacctcg agcagcgagc ggacgaccgc cacgtcgctg
     4861 aggtcgggca catcccgcag gatgctggtg gtctccccca gaagggcggc gaccatcgcc
     4921 ttggtggcga ggttcttcgc gcccttcacg tcgacacggc cgcgcaacgg cctgcctccg
     4981 cggatcgcga gcacggatcc ggcgggaacc ggagcctcgg aaactgctgc atcgcggagc
     5041 ggtgtcgtca ttcgggcctc ttcttcgtca ggacaggcgc ggcggtggcc gcgagcctct
     5101 catccaatca cggctggtgc cgtgggggct cttggccccc ggcccaccgc gcgcctcgtc
     5161 ctatcgaaca gggagcgtac gtggccgcca cgactcgcgg cgggcctcga actgcgcgat
     5221 cttgtcttcg ttgcgcagcg tgagcccgat gtcatcgagc ccttcgagga gccgccatct
     5281 agtgtaatcg tcgatcccga tggcggcctg gaagtccccg atcgtggcgg tgcgcgcctc
     5341 gaggtcgacc gtgatctcac ggcccggctc ctcgtcgatg agcgcccaga tccgctcgat
     5401 gtcggcttcg tcgactgtcg ccgccagcag gccctgcttt cccgagtttc cgcggaagat
     5461 gtcggcgaag cgcgggctga gcacgactcg gaagccgtag tcgcgcagtg cccacaccgc
     5521 gtgctcacgg ctggagccgg tgccgaagtc gggcccggcc accaggacgg acgccttctg
     5581 gaacggtccc tggttcagca cgaactccgg gtccttgcgc cactcgtgga agagggcgtc
     5641 gtcaaagccg gtcttggtga cgcgcttgag gaagacggcc gggatgatct ggtcggtgtc
     5701 gacgttggag cgcttcagag gcgcggccac gccggtgtga gtggtgaact tctccatggt
     5761 gctcaggcct ccaggtcgct cgggctggac agggtgccgc ggatggcggt ggcggccgcg
     5821 accagcggcg agaccaggtg cgtgcgtccg cccttgccct gccgcccctc gaagttgcgg
     5881 ttcgaggtcg acgcgcagcg ctcgcccggt gccagctggt cggggttcat gcccaggcac
     5941 atcgagcatc cggcgaaccg ccactcggcg ccgaacgcct cgacgatctt gtcgatgccc
     6001 tcggcctcgg cctcgagccg cacccgagcg gagccgggga cgaccatgac gcgcacgccg
     6061 tcggccttct tcttgccttc gatgaccgac gcgaaggcgc gcaggtcctc gatgcggctg
     6121 ttggtgcacg agcccatgaa caccgcatcg accttgacgt ccttcagcgg cgtgccgggg
     6181 gtcaggtcca tgtactgcag cgcgcgctcg gcagcggcgc gctcgttggc gtcggtgatc
     6241 tcggcggggt cggggacgga agccgacagc gagctgccct gacccgggtt ggtgccccag
     6301 gtgacgaagg gctcgagggt ggaggcgtcg atgtccacct cggcgtcgaa gacggcatcc
     6361 gggtcggagg ggagcgtgcg ccagtacgcg acagcatcct cccagtcctg cccctgcggg
     6421 gcgtgcggct tgtccttcac gtaggcgaac gtggtctcat ccggagcgac catgccggcc
     6481 cgagcccccg cctcgatcga catgttgcag atcgtcatgc ggccctccat cgagagggcg
     6541 cggatcgcgc tgccgcggta ctcgagcacg tagccctgtc ccccgccggt gccgatcttc
     6601 gcgatcacgg cgaggatgat gtccttggcg gtgacgcccg ggcggagctc gccctcgacg
     6661 ttgatcgcca tggtcttgaa cggcttcagc ggcagggtct gcgtggccat cacgtgctcg
     6721 acctcgctgg tgccaatgcc gtgcgccagc gcgccgaacg cgccgtgcgt ggaggtgtgc
     6781 gagtcgccgc acacgaccgt catgccgggc agcgttgcgc cgctttccgg gccgatcacg
     6841 tgcacgatgc cctggcgctt gctcaggaac gggaag
//
LOCUS       DATMSX010000013         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180214,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000013 DATMSX010000000
VERSION     DATMSX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180214"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..769
                     /locus_tag="VN041_00475"
     CDS             179..769
                     /locus_tag="VN041_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27554.1"
                     /translation="MSERSAPDLTVLPRFLAHLDPRDARAAHLARRLLDSGWQVQSVW
                     GPEQMDVWQLSLRRDSVAVRFGIERGISDGVLIGAAEATFNDLIPLSYAVLGWARASG
                     TRLPFEDPDDFRPDYAIYGAQAAEWVRAGEGDAFSRIVAARRHYLLRRHSVDQRLGEV
                     WLAQTKAWGVELLEAAAAPRTGEGGGRAGSAQTHGG"
     gene            complement(771..1223)
                     /locus_tag="VN041_00480"
     CDS             complement(771..1223)
                     /locus_tag="VN041_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU27555.1"
                     /translation="MGIADDAVEIRAQGWRTLAALHGMIEAELERALTAEAGLSVVEY
                     TVLDALNRQDGWHMRMQQLARAAALSPSATTRLVNRLEDRGLLTRILCMDDRRGIYTE
                     LTAAGQKLHDRARPVHDATLERVLAEAAGQPELAPMVDALNREPVTAS"
     gene            1341..2534
                     /locus_tag="VN041_00485"
     CDS             1341..2534
                     /locus_tag="VN041_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27556.1"
                     /translation="MPLGLIALAIGAFGIGLTEFVIMGLLPEVATDFGVSEATAGWLI
                     SGYALAVVVGALLLTALTIRLPRKPLLLGLVVLFIVGNVLTAVADTYTIAMVGRVIAA
                     LDHGAFFGVGSVVASGLVRPEKKAGAIAIMFTGLTAANVLGVPFGTFLGQQLGWRSTF
                     WAISLIGVVAFIGIAALVPAQRGAQQQVSLRAELGAFRSGQVWLSLTVTVLAYGGMFG
                     AFTYIAYTLTEVSGFASSSVPWLLVLFGAGLVVGNWLGGRLADRSIDRTLLAFIGALV
                     VVLVFFGFTAQWQIATIASLFLMGGFGFGTVPGLQSRIMQFAGQAPTLASGANIGAFN
                     VGNALGAWAGGVGIAAGLGYTAPIWIGAGITVAALVVMGIAIATAKAGGTASVSTSPE
                     LAHAN"
     gene            2557..3384
                     /locus_tag="VN041_00490"
     CDS             2557..3384
                     /locus_tag="VN041_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU27557.1"
                     /translation="MSIPTITLNNGIEMPQLGFGVFQVPDDETTAAVAEALRVGYRSI
                     DTAAVYGNEAGVGRAIADSGIDRSELFITSKVWNSDQGFDATLRAYDEALEKLGLEQL
                     DLYLIHWPTPERDLYLDTWRALERIHAEGRVRAIGVSNFQPAHLDRLVAASEVVPVVN
                     QVELHPALQNHDVIAANARHGIATEAWSPLAQGAVLRDPAVVAIAEAHGVSPAQAVLR
                     WHLQQGRIVIPKSVTPSRIAENFDVFGFELTADELGAIDALERDGRTGPHPDEFHAA"
     gene            complement(3445..5007)
                     /locus_tag="VN041_00495"
     CDS             complement(3445..5007)
                     /locus_tag="VN041_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU27558.1"
                     /translation="MPKILIVGGGYAGFYTAWKLEKHLRKGEADVTMVDPLPYMTYQP
                     FLPEVAAGSIEARHSVVAHRRHLKRTNVLTAKVTNINHAQKVATITPPVGEAYEFAYD
                     QIVVTAGAVSRTFPIPGIADNAIGLKTIEEAVAIRDKVMSNFDKAASLPAGPERDRLL
                     TVVVVGGGFAGIEVFAELRSLASSLVGKYPQISFEDTHFHLIEAMGRIMPEVSLKTSE
                     WVLKDLAKRGANVHLDTQVTGAIDGNVELSTGEVIPTDVIVWTAGVMANPTVVRGGDL
                     PVEERGRIQTRADLRVGTPEAFVDGAWAAGDVSAVPDLSGGGVGGFCVPNAQHAVRQA
                     KLLAKNLVAVLRGESPKEYFHKNLGAVAGLGLYNGVFQSGKLALKGFVAWVAHRGYHG
                     LAMPTWERKWRVLWGWWNNLWLGRDIVNLETVQNPRYVFEEFAARPRPAAPAAAPAAP
                     AEKPAPAQVADEKDVPVAKDAASKDAPAKDAAPKSAAAKAPAAKAAAKKAPAKKPAAK
                     KAPAKETADTKA"
     gene            complement(5033..>5281)
                     /locus_tag="VN041_00500"
     CDS             complement(5033..>5281)
                     /locus_tag="VN041_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27559.1"
                     /translation="AESQPEPEPTTAPVTVPPLPAADAPTEAGSPLLPSADSLRYGTL
                     PLIAVTVPGILIALGVTAAARRIRSARVPVRHNPDSEE"
BASE COUNT          774 a         1842 c         1784 g          881 t
ORIGIN      
        1 ttcggacagc ccgcctggtc gaacgacgat gccgacaggt tctggatagc agcggatgca
       61 ccgggcaagg tgttcgtccg cttcgagtac ggcgcggcgg gcgtggtgaa gatctcggtc
      121 acttcgtccg cggggtgagc ccacgattgc accgatccga ctccctacaa tcggcttcat
      181 gtctgagcga tccgctcccg atctgaccgt ccttcccagg ttcctcgccc acctggaccc
      241 ccgggatgca cgggctgcgc atctcgctcg ccggttgctt gattccggtt ggcaggtgca
      301 gtccgtgtgg gggcccgagc agatggatgt ctggcagctc agccttcggc gcgattcggt
      361 cgccgttcgc ttcggcatcg agcggggcat cagcgacgga gttctgatcg gagcagccga
      421 ggccaccttc aacgatctga tccccctgag ttacgcggta ctcggatggg cgcgcgccag
      481 cggaaccagg ctccccttcg aagaccccga cgatttccgg cctgactacg ccatctacgg
      541 cgctcaggca gcggagtggg tgcgagcggg agagggggac gcgttcagcc gcatcgtcgc
      601 ggcccgcagg cattaccttc tgcggcgcca ctcggtcgac cagagactcg gagaggtctg
      661 gctcgcgcag acgaaggcct ggggcgtgga gctgctcgag gccgcggcag cgccccgcac
      721 aggcgaaggc ggcggtcgcg ccggctcagc ccagacgcac ggcgggtgac tcagctcgcg
      781 gtgacgggct cgcggttcag cgcatcgacc atcggcgcca gctcgggctg accggcggcc
      841 tcggcgagga cccgctcgag ggtcgcatcg tgcacggggc gggcacgatc gtgcagcttc
      901 tgacccgcgg cggtcagctc ggtgtagatc ccgcgacggt cgtccatgca gaggatgcgt
      961 gtgagcagcc cgcggtcctc gagccggttg accagacgcg tggtcgcgct cggactcagc
     1021 gctgcggcac gggcgagctg ctgcatccgc atgtgccagc catcctgccg gttcagcgcg
     1081 tcgagcaccg tgtactcgac gaccgacagg ccggcttccg ccgtgagggc gcgttcgagc
     1141 tcggcctcga tcatgccgtg cagcgcggcc agcgtgcgcc agccctgtgc cctgatctcg
     1201 acggcgtcat ccgcgatgcc catgggagct cctctcagcg atagttgctt gcgctggtta
     1261 gttgcgtgtg caatcattgt tcctgcatcc gcaaaatcca gcgtctgcaa ctacttggca
     1321 agtctaacag gagaaccctc atgcctctcg gactcatcgc cctcgccatc ggagcgttcg
     1381 gcatcggtct caccgaattc gtcatcatgg gtctgctgcc ggaggtcgcg accgacttcg
     1441 gcgtcagcga ggcgaccgcg ggctggctga tctccggcta cgccctggcc gtcgtggtcg
     1501 gcgccctgct gctgaccgcg ctcaccatcc gcctcccccg caagcccctg ctgctcggac
     1561 tggtcgtgct gttcatcgtc ggcaacgtgc tcaccgccgt cgccgacacc tacacgatcg
     1621 ccatggtcgg ccgcgtcatc gccgcgctcg accacggggc gttcttcgga gtgggttccg
     1681 tggtcgcctc gggcctggtg cgccccgaga agaaggcggg cgcgatcgcc atcatgttca
     1741 cgggactcac cgcggcgaac gtgctgggcg tgccgttcgg caccttcctc ggccagcagc
     1801 tcggctggcg gtccaccttc tgggccatct ccctgatcgg cgtcgtcgcc ttcatcggca
     1861 tcgccgccct cgtccccgcg cagcgcggag cgcagcagca ggtcagcctg cgcgccgagc
     1921 tcggcgcatt ccgctccggc caggtctggc tgtccctcac cgtgaccgtg ctcgcgtacg
     1981 gcggcatgtt cggcgcattc acctacatcg cgtacacgct caccgaggtc agcggattcg
     2041 cctccagctc ggttccctgg cttctcgtgc tgttcggcgc cggcctggtc gtcggcaact
     2101 ggctgggcgg gcgcctggcc gatcgctcga tcgaccgcac cctgctcgcc ttcatcggcg
     2161 ccctcgtcgt cgtactggtc ttcttcggct tcaccgcgca gtggcagatc gccacgatcg
     2221 cgtcgctgtt cctgatgggc ggattcggct tcgggacggt cccgggcctg cagagccgca
     2281 tcatgcagtt cgcgggccag gccccgacgc tggcatccgg tgccaacatc ggcgccttca
     2341 acgtcggcaa cgccctgggt gcgtgggccg gcggagtcgg catcgcggcc ggcctcggct
     2401 acacggcccc gatctggatc ggcgccggca tcaccgtggc cgccctcgtc gtgatgggca
     2461 tcgccatcgc caccgcgaag gcgggcggta cggcatccgt ctccacctcc cctgaactcg
     2521 cccacgcgaa ctgacccacc tcctcgaaag gaaatcatgt ccatccccac aatcactctg
     2581 aacaacggca tcgagatgcc gcagctcggc ttcggcgtct tccaggtgcc cgatgacgag
     2641 acgactgcgg ccgtcgccga ggcgctgcga gtcggctatc gcagcatcga caccgcggcc
     2701 gtctacggca atgaggccgg cgtgggccgc gccatcgccg actcgggcat cgaccggtcc
     2761 gagctgttca tcacctcgaa ggtctggaac tcggaccagg gcttcgacgc cacgctccgc
     2821 gcctatgacg aggcgctgga gaagctcggc ctcgaacagc tggacctgta cctgatccac
     2881 tggcccacgc cggagcgcga cctctacctg gacacctggc gggcactcga gcggatccac
     2941 gccgaggggc gcgtgcgcgc catcggcgtg tcgaacttcc agcccgcgca cctggaccgc
     3001 ctggtcgcgg catccgaggt cgtcccggtg gtcaaccagg tcgagctgca cccggccctg
     3061 cagaaccacg acgtgatcgc ggcgaacgcc cgtcacggca tcgcgaccga ggcgtggagc
     3121 ccgctcgccc agggcgccgt gctgcgcgac ccggccgtcg tggcgatcgc cgaggcgcac
     3181 ggcgtctccc ccgcacaggc cgtgctgcgc tggcacctgc agcagggccg catcgtgatc
     3241 ccgaagtcgg tgacgccgtc gcgcatcgcg gagaacttcg acgtgttcgg cttcgagctc
     3301 accgctgacg agctgggtgc gatcgacgcg ctcgagcgag acggccgcac cggcccgcac
     3361 ccggacgagt tccacgccgc ctgagcgacg cccgcacagc gaaaggcccg gatccgatcg
     3421 gatccgggcc tttcagcgtc gatgtcacgc cttggtgtcg gcggtctcct tggcgggagc
     3481 cttcttggca gccggcttct tggcgggagc cttcttggcg gcggccttcg cggccggggc
     3541 cttggccgca gcagacttgg gcgccgcgtc cttcgccggc gcatccttcg atgcggcatc
     3601 cttcgcgacc ggaacgtcct tctcgtcggc gacctgcgcc ggagcgggct tctcagcagg
     3661 agcggccggt gccgcagcgg gagccgccgg acggggccga gccgcgaact cctcgaagac
     3721 gtagcgcggg ttctgcacgg tctcgaggtt cacgatgtcg cgaccgagcc acaggttgtt
     3781 ccaccagccc cacagcacgc gccacttgcg ctcccacgtc ggcatcgcga gaccgtggta
     3841 gccacggtgc gcgacccagg cgacgaagcc cttgagcgcg agcttgccgg actggaagac
     3901 accgttgtag aggccgagac cggcgacggc accgaggttc ttgtggaagt actccttcgg
     3961 gctctcaccg cgcaggacgg cgacgaggtt cttcgcgagg agcttcgcct gacgcacggc
     4021 gtgctgggcg ttcggcacgc agaacccgcc gacgccaccg cccgagaggt cgggaacagc
     4081 cgacacgtct ccggcagccc aggctccgtc gacgaacgcc tcgggcgtgc ccacgcgcag
     4141 gtcggcacgg gtctggatgc gtccacgctc ctcgaccggg agatcgccgc cacgcacgac
     4201 ggtggggttc gccatgacac ccgcagtcca gacgatgacg tcggtcggga tcacctcacc
     4261 ggtggagagc tcgacgttgc cgtcgatggc cccggtgacc tgcgtgtcga ggtgcacgtt
     4321 ggcgccgcgc ttggcgaggt ccttgagcac ccactcgctc gtcttcaacg agacctcggg
     4381 catgatgcgc cccatcgcct cgatgaggtg gaagtgcgtg tcctcgaagc tgatctgcgg
     4441 gtacttgccc accagcgagg aggcgagcga acgaagctcc gcgaacacct cgatgcccgc
     4501 gaacccgccg cccacgacca cgacggtcag cagacgatcg cgctcggggc cagccggaag
     4561 ggaagcggcc ttgtcgaagt tcgacatgac cttgtcgcgg atcgcgacgg cttcctcgat
     4621 cgtcttcagc ccgatcgcgt tgtcggcgat gcccgggatc gggaaggtgc gcgagacggc
     4681 acccgccgtg acgacgatct ggtcgtaggc gaactcgtac gcctcaccca cgggcggcgt
     4741 gatcgtggcg accttctgcg cgtggttgat gttcgtcacc ttggcggtga ggacgttcgt
     4801 gcgcttgagg tggcggcggt gggcgacgac cgagtgacgc gcctcgatgg aaccggcggc
     4861 gacctcggga aggaacggct ggtacgtcat gtacggcagc gggtcgacca tcgtcacgtc
     4921 ggcctcaccc ttgcgcaggt gcttctcgag cttccacgcg gtgtagaaac ccgcgtaccc
     4981 tccgccgacg atcaggatct tgggcacagt gctgttctga ggcacagaag aactactcct
     5041 cggagtcggg attgtggcgt acgggaacgc gcgccgatcg gatgcgccgg gcagctgcgg
     5101 tgacgccaag cgctatcagg ataccaggga ccgtgaccgc gatgagcggc agggtaccgt
     5161 agcgcagtga atcggcgctg ggaagcagcg gtgaaccggc ctccgtcggt gcgtcggcgg
     5221 ccggaagcgg cggcacggtc accggtgcgg tggtcggttc cggctccggc tgcgactcgg
     5281 c
//
LOCUS       DATMSX010000014         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_9694,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000014 DATMSX010000000
VERSION     DATMSX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9694"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN041_00505"
     CDS             complement(<1..765)
                     /locus_tag="VN041_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU27560.1"
                     /translation="MTAITRPGKIIAIHLNYVSRADQRGRRPAAPSYFFKPASSVGVS
                     GGTVERPAGTELLAFEGEIALIIGTPARRVSVEDAWSHVASVTASNDLGLYDLRANDK
                     GSNVRSKGGDGYTPLGPELIDAASVDPGNLRVRAWVNGELRQDDSTTGLLFPLPQLVA
                     DLSQHFTLEPGDVILAGTPAGSSVIVPGDVVEIEVDAPDAPGAPSSGRLVTTVTQGEV
                     AFDPALGSLPAVDDLQRTEAWGSREAAGLPETDSEPA"
     gene            895..2310
                     /locus_tag="VN041_00510"
     CDS             895..2310
                     /locus_tag="VN041_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27561.1"
                     /translation="MSGKSEDGFTPTGTIASAADRRRVVVATVVGTTVEWYDFFIYAF
                     AAATVFAALFFEPMGKDFAQIVSFFSVGVSFLFRPLGAFLAGHFGDKIGRRPMLVLTL
                     LLMGGATTLVGVLPTHALIGVWAPILLILLRVIQGISAGGEWGGAVLMAVEHAPKHKR
                     GLFGASPQIGVPIGLLLASMVLALTETIFPGPKPGSDVALADTAFGQIGWRIPFLLSV
                     VLIGVGYWVRRRVSESPVFQEIAERKEETRMPIVQLLRHHLPLVIIAALVFAGNNAVG
                     YMTTGGYVQHYATDPNGPIGLDTGAVFWIVALSGISWLFFTWFAGWISDRIGRRNTYI
                     IGWVLQLIGVFLLFFLTNLGTLGSLAAALVILTIGLGFTYGPQAALYSELFPASIRFS
                     GVSISYAIGAIVGGAFSPLIAQAIFQNTHSTTGITWYLAGMTVIGLLATLLLRDRTGI
                     PLGPENEAEQAKSPIYGMSKA"
     gene            2425..>2805
                     /locus_tag="VN041_00515"
     CDS             2425..>2805
                     /locus_tag="VN041_00515"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HWU27562.1"
                     /translation="MLITEQLHLLLLRPDGRGESSAWSYRAYGEVAAVIVDLALAGRL
                     TVTEEKRPVVHVVSPAPTGHPVLDAALARLAPLSGRRLGSLVSRSALDPKDEVIASLI
                     AQGVLGHGERGFFGLGAERTPEAEP"
BASE COUNT          391 a          961 c          978 g          475 t
ORIGIN      
        1 cgcgggctcg gagtcggtct cggggagccc tgccgcctcg cgcgagcccc aggcctcggt
       61 gcgctgcaga tcgtcgacgg ccggcagcga gccgagggct gggtcgaagg cgacctcgcc
      121 ctgcgtcacc gtcgtgacca ggcgaccaga cgacggtgca ccaggggcat ccggagcatc
      181 cacctcgatc tccacgacat cacccgggac gatcacgctg gatcccgcgg gcgtgccggc
      241 gaggatcacg tcaccgggct cgagggtgaa gtgctgcgag aggtcggcga ccagctgggg
      301 gagcgggaag agcaggcccg tcgtgctgtc gtcctgacgc agctcgccgt tgacccaggc
      361 ccgcacgcgc aggttccccg ggtcgacgga tgccgcatcg atcagctcgg ggccgagcgg
      421 ggtgtacccg tcgccgccct tggatcggac gttggagccc ttgtcgttgg cgcgcaggtc
      481 gtacaggccg aggtcgttgg atgcggtgac ggatgccacg tgcgaccagg cgtcctcgac
      541 ggacacccgg cgagcggggg tgccgatgat cagggcgatc tcaccctcga aggcgagcag
      601 ctcggtgccg gcggggcgtt cgaccgtgcc gccgctgacg ccgacggagc tcgcgggctt
      661 gaagaagtag gagggtgctg cggggcggcg gccgcgctga tcggcgcgcg agacgtagtt
      721 caggtggatc gcgatgatct tgcccggtcg ggtgatcgcc gtcatggatg cgcctcctcg
      781 cgttcgcgca cagggaggat gccttgtgcg tcgtatgcga aatcgtatat catcgtcgca
      841 tcctggggca agcatcacgt ggaatccccg acagtcacct cgaaggagag agacatgagc
      901 ggcaaatcag aggacgggtt caccccgacc ggaaccatcg cgagcgccgc agaccggcgt
      961 cgcgtcgtcg tcgccaccgt ggtcggcacc accgtggagt ggtacgactt cttcatctac
     1021 gcgttcgccg cggccaccgt cttcgcagcc ctgttcttcg agccgatggg caaggacttc
     1081 gcgcagatcg tgtcgttctt cagcgtcggc gtcagcttcc tgttccggcc gctgggcgca
     1141 ttcctggccg ggcacttcgg tgacaagatc ggccgtcgcc cgatgctggt gctcacgctc
     1201 ctgctgatgg gcggcgcgac cacgctcgtc ggcgtgctgc ccacgcacgc gctgatcggc
     1261 gtctgggcgc cgatcctgct gatcctgctg cgcgtgatcc agggcatctc ggccggcggc
     1321 gagtggggcg gcgccgtgct gatggccgtg gagcatgcgc ccaagcacaa gcgcgggctg
     1381 ttcggggcat ccccgcagat cggcgtgccg atcgggctgc tgctggcgtc gatggtgctg
     1441 gcactgaccg agacgatctt ccccggcccg aagccgggtt cggacgtcgc gctcgccgac
     1501 accgccttcg ggcagatcgg ctggcgcatc cccttcctgc tgtcggtcgt gctgatcggc
     1561 gtcggctact gggtgcggcg tcgggtctcg gagagcccgg tgttccagga gatcgccgag
     1621 cgcaaggagg agacgcgcat gccgatcgtc cagctgctgc gccaccacct gccgctcgtg
     1681 atcatcgcgg cactcgtgtt cgcgggcaac aacgccgtcg gctacatgac caccggcggt
     1741 tacgtgcagc actacgccac cgaccccaac gggccgatcg ggctggacac cggcgcggtc
     1801 ttctggatcg tcgcgctgtc ggggatcagc tggctgttct tcacctggtt cgcgggctgg
     1861 atctccgaca ggatcgggcg ccgcaacacc tacatcatcg gctgggtgct gcagctgatc
     1921 ggcgtcttcc tgctgttctt cctgaccaat ctcggtacgc tcggctcgct cgccgcggcg
     1981 ctcgtgatcc tcaccatcgg gctcggattc acatacgggc cgcaggccgc gctgtactcg
     2041 gagctgttcc cggcgtccat ccggttctcc ggcgtctcga tctcgtacgc gatcggcgcc
     2101 atcgtgggcg gcgcgttctc gccgttgatc gctcaggcga tcttccagaa cacgcattcc
     2161 accaccggca tcacctggta cctggccggc atgaccgtga tcggtctcct cgccaccctg
     2221 ctgctgcgcg accgcaccgg catcccgctg gggccggaga acgaggccga gcaggcgaag
     2281 tccccgatct acgggatgtc caaggcctga ggccccggat cgccagccgg aagggcggcc
     2341 ccctgcgggt cgtccttccg gtggaccccg catatccctc tcctgacgga tgccggcggc
     2401 gcgagcgctc catagcgtgg agggatgctg atcaccgagc aactgcatct tctgctgctt
     2461 cgaccggacg ggcgcggcga gagctccgcg tggagctacc gtgcgtacgg cgaggtcgcc
     2521 gccgtcatcg tcgatctcgc gctcgccgga cgactcaccg tcacggagga gaagcgtccc
     2581 gtcgtgcacg tcgtgtcgcc ggcaccaacg gggcatccgg tgctggacgc cgcgctcgcc
     2641 cggctcgccc cgctgagcgg ccgacgactc ggctcgctcg tgtcgcggtc ggcgctggac
     2701 ccgaaggacg aggtgatcgc ctcgctgatc gcacagggcg tgctcggcca tggcgagcgc
     2761 ggcttcttcg gcctcggtgc cgagcgcaca cccgaggccg agccc
//
LOCUS       DATMSX010000015         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_38145,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000015 DATMSX010000000
VERSION     DATMSX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38145"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1940
                     /locus_tag="VN041_00520"
     CDS             <1..>1940
                     /locus_tag="VN041_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWU27563.1"
                     /translation="VESAAEAEQSVESEQPAAEAETSAEAETPAGEKPADAETPAEEK
                     PAEPEQPSVPEPVTAVSLGLLPEVFVSKVSTQLHFYAPEIVPLPASAAHDDEDDERGS
                     AFSRRGSSTRRRRGGDGDEGRDEPRQRQRTVEYITEPKAIKGSTRLEAKKQRRRDGRD
                     AGRRRPVVTEAEFLARRESVDRMMVVRAKNGRTQIGVLEDNVLVEHYVARNQDASLIG
                     NVYLGRVQNVLPSMEAAFVDIGRGRNAVLYSGEVDWDAVETGNQPRRIELALKPGDRV
                     LVQVTKDPVGHKGARLTSQISLPGRYLVYVPGGSMNGISRKLPDNERARLKRILKEVL
                     PESSGVIVRTAAEGATEEQLTRDVQRLTSQWEHIQKLVQTQQAPALLHAEPDLLVKIV
                     RDVFNEDFTRMTIQGDDAHRTISAYLESVAPDLLERVETYTDEVDPFDAYRITEQIEK
                     ALDRKVWLPSGGSLVIDRTEAMTVVDVNTGKFVGSGGNLEETVTKNNLEAAEEIVRQL
                     RLRDIGGIIVVDFIDMVLESNRDLVLRRLVECLSRDRTKHQVAEVTSLGLVQMTRKKL
                     GLGLLETFSEPCEVCAGRGVVVHHDPVVKHRGGPAGSSQGRRSRGSSAPQQAPQAAPA
                     QPAAAANGGTHSITEQIEKALDR"
BASE COUNT          333 a          713 c          646 g          248 t
ORIGIN      
        1 gtcgagagcg ccgcggaggc cgagcagtcc gtcgagtccg agcagcctgc cgctgaggcc
       61 gagacgtccg ccgaggccga gacgcccgcg ggggagaagc ctgccgacgc agagacgccc
      121 gcggaggaga agcccgccga accggagcaa ccgtccgtgc ccgagcccgt caccgcggtg
      181 agcctgggcc tgctgcccga ggtcttcgtc tccaaggtct ccacgcagct gcacttctac
      241 gctcccgaga tcgtcccgct gccggcatcc gccgctcacg acgatgagga cgacgagcga
      301 ggctcggcgt tctctcgccg cggcagcagc actcgtcgcc gccgcggcgg cgacggagac
      361 gagggtcgtg acgagccgcg tcagcgtcag cgcacggtcg agtacatcac cgagccgaag
      421 gccatcaagg gctccacccg cctcgaggcc aagaagcagc gccgccgcga cggtcgcgac
      481 gccggccgcc gccgtccggt ggtcaccgag gccgagttcc tcgcccgccg cgagtccgtc
      541 gaccgcatga tggtcgtgcg cgccaagaac ggccgcaccc agatcggcgt gctcgaggac
      601 aacgtgctcg tcgagcacta cgtcgctcgc aaccaggacg cctcgctgat cggcaacgtc
      661 tacctcggac gcgtgcagaa cgtgctgccg agcatggagg ccgccttcgt cgacatcggc
      721 cgcggccgca atgccgtgct ctactccggc gaggtcgact gggacgccgt ggagacgggc
      781 aaccagccgc gtcgcatcga gctcgccctc aagcccggtg accgcgtgct cgtccaggtc
      841 accaaggacc cggtcggcca caagggcgcc cgcctcacca gccagatctc gctgcccggc
      901 cggtacctgg tgtacgtgcc aggcggctcc atgaacggca tctcgcgcaa gctgcccgac
      961 aacgagcgcg cccgcctgaa gcgcatcctc aaggaggtgc tgccggagtc gtccggcgtg
     1021 atcgtccgca ccgcggccga gggcgccacc gaggagcagc tgacccgcga cgtgcagcgg
     1081 ctcacgtccc agtgggagca catccagaag ctcgtgcaga cccagcaggc gcccgcgctc
     1141 cttcacgctg agcccgacct gctggtcaag atcgtgcgcg acgtcttcaa cgaggacttc
     1201 acccggatga ccatccaggg cgatgacgcg caccgcacca tcagcgccta cctcgagagc
     1261 gtggctcccg acctgctgga gcgggtcgag acgtacaccg acgaggtgga cccgttcgac
     1321 gcgtaccgca tcaccgagca gatcgagaag gccctggacc gcaaggtctg gctgccctcg
     1381 ggcggttcgc tggtcatcga ccgcaccgag gcgatgacgg tggtcgacgt caacaccggc
     1441 aagttcgtcg gctcgggcgg aaacctcgag gagaccgtca ccaagaacaa cctcgaggcc
     1501 gccgaggaga tcgtccgcca gctgcgcctg cgcgacatcg gcggcatcat cgtcgtcgac
     1561 ttcatcgaca tggtgctcga gtccaaccgc gacctcgtgc tgcgccgcct ggtggagtgc
     1621 ctgagccgcg accgcacgaa gcatcaggtc gcggaggtca cctcactcgg tctcgtgcag
     1681 atgacgcgca agaagctcgg cctgggcctg ctggagacgt tcagcgagcc ctgcgaggtg
     1741 tgcgccggcc gcggtgtcgt cgtgcaccac gacccggtcg tcaagcaccg cggcggaccc
     1801 gccggcagca gccagggacg tcgctcgcgc ggctcctccg ctccgcagca ggctccgcag
     1861 gccgctcccg ctcagcccgc tgcagcggcg aacggaggca cccacagcat caccgagcag
     1921 atcgagaagg ccctggaccg
//
LOCUS       DATMSX010000016         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180241,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000016 DATMSX010000000
VERSION     DATMSX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180241"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..1453
                     /locus_tag="VN041_00525"
     CDS             458..1453
                     /locus_tag="VN041_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWU27564.1"
                     /translation="MSNQESNPTESVLEPGALCTDVEVLTPREVPLGGIRAMTVYRTL
                     PQKQRSLVGAWCFLDHYGPDDVADTGGMEVPRHPHTGLATVSWLFTGRIDHLDSNGVA
                     APVLPGELNLMIAGQGITHQEISTPDTTVLHGVQLWYALPESTRFSEHAFAHYAPEPV
                     ELQGVSIRVFIGSLAGSTSPVDTRTPDLLGAELVLEPGAQVVLDVRRDFEHAALAESG
                     SIMVNDTPVRHRELGYTPTGADTMQIAAGPEGARVILLGGVPLGEQIVMWWNFIGRSH
                     DEIVEFRRRYQAELGFEPADPRDAGKPELFGPYAEAQLAPLPAPPMPTIRLRARE"
     gene            complement(1471..1833)
                     /locus_tag="VN041_00530"
     CDS             complement(1471..1833)
                     /locus_tag="VN041_00530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27565.1"
                     /translation="MTVERFTVQDLPAESRYVLIDRGEDGTDSREIGEESYVDVGDER
                     VLFHTGVSEDYAGQGLASKLVRAVVDDVVTAGKSIVPVCPYVAAWMPKHPEYDEHVVA
                     RRADHIAAIRAQEEGREA"
     gene            complement(1830..2303)
                     /locus_tag="VN041_00535"
     CDS             complement(1830..2303)
                     /locus_tag="VN041_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU27566.1"
                     /translation="MRPYLDKSAPDVWKAVSAFSATVKQHAAAAGLSVQESEYIKLRA
                     SQLNACAYCLDVHSRESREAGIVQQKIDLLPAWRESTLYTEREKAVLAVAEAATRMPL
                     SEESKADLAGAAAVLGEQAFAAAEWVAITINAFNRVSILSEHPVRPRGVDGEVLS"
     gene            2436..2993
                     /gene="msrA"
                     /locus_tag="VN041_00540"
     CDS             2436..2993
                     /gene="msrA"
                     /locus_tag="VN041_00540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWU27567.1"
                     /translation="MTSGSSDTGTITRQAGAETAVLAGGCFWGMEDLIRRQPGVLGTR
                     VGYTGGENDHATYRNHPGHAEAVEIVFDPAQTTYRDILAFFFQIHDPSTLNRQGNDIG
                     TSYRSAIFPLDAEQERVARDTIADVDASGLWPGAAVTTIEQPGPFWEAEPEHQDYLIR
                     YPDGYTCHFPRAGWVLPRRDAEASA"
BASE COUNT          487 a         1133 c         1086 g          483 t
ORIGIN      
        1 gcgactgcgg gtgccgcggc tgcggcgctc aggagtccgg agcctgccgt cgcgacggcg
       61 agggcggctc cgccgcggag cagctgtctg cgcgacgctt tcagctcggt cgtggggtcg
      121 gtgtgttcca tcgtgctctc ctcgtgcgtc gttgcaggcc gggaatgtcg gccacccatt
      181 tggtatacca gaccatcggg gcaacgatag cggtgatgac cgctgtcgga aagggcggcg
      241 agcaaatggc ggaggttcgg gtaaagggtc ttcgacgttt ggtatgccaa tttgcgtcct
      301 ccttccgacg ttcgacgggg aggtgctgca ggccatcggc gttcctgtcg gcgtgctgct
      361 ctccgcgatc agcagcgcct gaggccgcgc gacccgaccg cccgcgggac tggctacgct
      421 ggcaggagtc gcgtcacgga tgccgaggag aacccggatg agcaaccagg agtcgaaccc
      481 gacggagagc gtgctggaac cgggcgcgct gtgcaccgac gtcgaggtgc tcacccctcg
      541 tgaggtgcca ctcggcggca tccgcgcgat gaccgtctac cgcaccctgc cgcagaagca
      601 gcgctcgctg gtcggggcgt ggtgcttcct ggatcactac gggccggatg acgtggccga
      661 caccggcggc atggaggtcc cgcggcatcc gcacaccgga ctcgcgaccg tctcgtggct
      721 gttcaccggc cgcatcgacc acctcgactc caacggggtc gctgctcccg tgctccccgg
      781 cgagctcaac ctgatgatcg ccgggcaggg catcacccac caggagatca gcacccccga
      841 cacgaccgtg ctgcacggcg tgcagctctg gtacgcgctg cccgagtcca cgaggttctc
      901 cgagcacgcg ttcgcgcact atgcgcccga accggtggag ctgcagggcg tgagcatccg
      961 cgtgttcatc ggctccctgg cggggtcgac ctcgccggtc gacacccgca cccctgatct
     1021 gctgggagcc gagctggtgc tggagccggg agcgcaggtc gtgctcgacg tgcgtcgtga
     1081 cttcgagcac gccgctctcg ccgagagcgg gtcgatcatg gtcaacgaca cgccggtacg
     1141 gcaccgcgag ctcggctaca cacccaccgg cgccgacacc atgcagatcg cggcagggcc
     1201 cgagggtgcc agggtgatcc tgctcggcgg cgtgccgctg ggggagcaga tcgtgatgtg
     1261 gtggaacttc atcggccgca gccacgacga gatcgtcgag ttccgccgcc gctatcaggc
     1321 cgagctggga ttcgagccgg cggaccccag ggatgccgga aagccggagc tgttcggccc
     1381 gtacgccgag gcgcagctcg ccccgctgcc cgcaccgccg atgcccacca tccggctgcg
     1441 ggcacgggag taggcgcacc gccgcagggg tcaggcctcg cggccctcct cttgcgccct
     1501 gatcgccgcg atgtggtccg cccggcgcgc cacgacgtgc tcgtcgtact ccggatgctt
     1561 cggcatccac gccgcgacgt acgggcacac gggcacgatc gacttacctg ccgtcaccac
     1621 gtcgtcgacg accgcgcgga cgagcttgga tgccaggccc tgacctgcgt agtcctcgga
     1681 tacgccggtg tggaagagca cgcgctcgtc cccgacatcc acgtacgact cctcgccgat
     1741 ctccctggaa tccgtgccgt cctcgccgcg gtcgatcagc acgtagcgcg attcggcggg
     1801 gaggtcctgc acggtgaagc gctcgaccgt catgacagca cctctccgtc gaccccgcgc
     1861 ggacgcaccg ggtgctcact gaggatggag acgcgattga acgcgttgat cgtgatcgcg
     1921 acccactcgg cggcggcgaa tgcctgctcg cccagcaccg cggcggcgcc ggccagatcg
     1981 gccttggact cctcgctcag cggcatccgc gtcgccgcct ccgcgacggc cagcaccgcc
     2041 ttctcgcgct cggtgtagag ggtgctctcg cgccaggccg gcagcaggtc gatcttctgc
     2101 tgcacgatgc cggcctcccg cgactcgcgg gagtgcacat cgaggcagta ggcgcaggcg
     2161 ttcagttgcg acgcgcgcag cttgatgtac tcggactcct gcaccgacag gcctgcggcc
     2221 gccgcgtgct gcttgaccgt cgcggagaag gcgctgaccg ccttccagac gtcgggtgcg
     2281 gacttgtcca gatacggacg catagctctc ctcggatgct cgggatgcgg gccgggcccg
     2341 ctctcagcac caacctagac ggcccggccc tcattcccca gccagggggg agaatgggac
     2401 gggcacaccc gcacgaacac cagaggagca ccgtgatgac cagcggcagc agcgacaccg
     2461 gaacgatcac ccgccaggcg ggagcggaga ccgccgtcct cgcaggaggc tgtttctggg
     2521 gcatggagga cctgatccgc cggcagcccg gcgtgctcgg cacgcgagtc ggctacaccg
     2581 gtggcgagaa cgaccacgcc acctaccgga accaccccgg tcacgccgag gccgtcgaga
     2641 tcgtcttcga cccggctcag acgacctacc gcgacatcct cgcgttcttc ttccagatcc
     2701 atgacccgtc cacgctgaac cggcagggca acgacatcgg caccagctac cgctccgcga
     2761 tcttcccgct ggacgcggag caggagcgcg tggccaggga cacgatcgcc gacgtggacg
     2821 cctcgggact ctggccgggt gccgccgtca cgaccatcga gcagcccggt ccgttctggg
     2881 aggccgaacc cgagcaccag gactatctga tccgctaccc ggacggctac acctgccact
     2941 tcccgcgcgc cggctgggtc ctgccgcgcc gtgacgccga ggccagcgcc tgatccgcgt
     3001 cggacctggt gacggggtct gaggggatgg caggcagcgc ccagcccgat cgcatcgcgg
     3061 tacgcggcgc gcgcgtgcac aacctgcggg gcgtggacgt cgacatcccg ttgcgatcgc
     3121 tggtgggcat cgccggcgtc tccggctcgg gcaagtcgtc gctcgccctc ggcgtgctgt
     3181 acagatcgg
//
LOCUS       DATMSX010000017         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_142355,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000017 DATMSX010000000
VERSION     DATMSX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142355"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1527)
                     /locus_tag="VN041_00545"
     CDS             complement(<1..>1527)
                     /locus_tag="VN041_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27568.1"
                     /translation="EEVDVDKLSMLLVAVLRPDDVEVDKLLTLLFVELRPVDRLVIPL
                     VAVLRPVERLSMPLVAVLRPDEVEVDRLSMLLVAVLKPDDVEVDRLEMLLVAVLKPDD
                     VEVERLSRLLFVELRLVDKLVMPLVAVLKPLDVDVDKLSRLLLVVLRPVDRLEIPLVA
                     VLRPDEVDVDRLEMLLVAVLRPDDVDVDRLLTLLLVVLKPVDRLEIPLVAVLKPLDVE
                     VDKLLRLLFVVLKPVDRLPMSVERPSMRLLVVLRPLDVDVDKLSMLLVAVLKPDDVEV
                     DKLLTLLFVELRLVDKLEIPLVAVLRPDEVDVDRLSMPLVAVLRPDDVEVERLSMLLV
                     AVLKPDEVEVDRLLTLLFVELRLVDKLEIPLVAVLKPDDVEVDRLEIPLVAVLKPLDV
                     DVDKLSRLLLVVLRPDDVDVDRLSMLLVAVLKPDDVEVDKLLTLLFVVLRPVERLEMP
                     LVAVLKPEEVDVDKLSMLLVAVLRPDDVEVDKLPTLLFVVLRPVDRLEIPLVAVLRPD
                     D"
BASE COUNT          308 a          521 c          421 g          277 t
ORIGIN      
        1 gtcgtcgggt ctcagcacgg cgacgagcgg gatctcgagc ctgtccacgg gcctgagcac
       61 cacgaacagc aacgtcggta gcttgtcgac ttcgacgtcg tcgggtctga gcacggccac
      121 gagcagcatc gatagcttgt cgacgtcgac ctcttctggc ttgagcacgg cgaccagtgg
      181 catctcgagc ctctcaacgg gcctgagcac cacgaacagc aacgtcagca gcttgtcgac
      241 ctcgacgtcg tcgggcttga gcacggccac gagcagcatc gacagcctgt cgacatcgac
      301 gtcgtctggc ctgagcacca ccaatagcaa cctggacagc ttgtcgacat ccacgtcgag
      361 cggcttgagc actgcaacca gtggtatttc gagcctgtcg acttcgacgt cgtctggttt
      421 gagcactgcg accagcggta tctccagctt gtccacgagc ctcagctcca cgaacagcaa
      481 cgtgagcagc ctgtcgactt cgacctcgtc gggcttgagc acggcgacga gcagcatcga
      541 cagtctctct acttcgacat cgtcgggtct gagcacggcc acgagcggca tcgacagcct
      601 gtcgacgtct acctcgtccg gcctgagcac cgccaccagc ggtatctcca gcttgtccac
      661 gagcctcagc tccacgaaca gcagcgtgag cagcttgtcg acctcgacgt cgtctggctt
      721 gagcacagcc acgagcagca tcgacagctt gtcgacgtct acgtcgagcg gtctgagtac
      781 gaccaacagc cgcatcgatg gtctctcaac cgacatcggt agcctgtcga ccggtttgag
      841 caccacgaac agcaacctca acagcttgtc gacctcgacg tcgagcggct tgagcacggc
      901 cacgagtggt atctccagcc tgtccaccgg cttgagcacg accaacagca acgtgagcag
      961 cctgtcgacg tccacgtcgt cgggtctgag caccgccacg agcagcatct cgagcctgtc
     1021 gacgtcgacc tcgtctggcc tgagtactgc caccagcggt atctcgagtc tgtctaccgg
     1081 cctgagcacc accaatagca acctggacag cttgtcgaca tccacgtcga gcggcttgag
     1141 cactgcaacc agtggcatca ccagcttgtc cacgagcctc agctccacga acagcaacct
     1201 ggacagtctc tctacttcga catcgtcggg cttgagcacc gccaccagca gcatctcgag
     1261 cctgtcgact tcgacgtcgt ctggcttgag tacagccacg agcagcatcg acagcctgtc
     1321 gacctcgacc tcgtcgggcc tgagcacggc aacgagcggc attgacagcc tctcgaccgg
     1381 cctgagcacg gccaccagcg gtatcacgag cctgtcgacg ggcctgagct ccacgaacag
     1441 caacgtcagc agcttgtcga cctcgacgtc gtcgggtctg agcacggcca cgagcagcat
     1501 tgatagcttg tcgacgtcga cctcttc
//
LOCUS       DATMSX010000018        15923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_310, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000018 DATMSX010000000
VERSION     DATMSX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 15923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15923
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VN041_00550"
     CDS             complement(<1..541)
                     /locus_tag="VN041_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27569.1"
                     /translation="MAHAGFEVSAPADVPMPARVPGPRGVGRLISLLGIALIAFVLWR
                     DTGSSSPWMTVIAVTALVAWLILAALPAGASLWLRLVLLALMVIGGAVASTTTTGVAS
                     AVLIVGIVTVIGSTVLSLAVGFAFVASGLLAIAFCALTAPPMLQVLASIAGIAAGVLI
                     GLARRQARQATERERRTAQE"
     gene            complement(581..1093)
                     /locus_tag="VN041_00555"
     CDS             complement(581..1093)
                     /locus_tag="VN041_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27570.1"
                     /translation="MDISVLTTILISLAVIVWLSIRQTTWRTLDPGRAFRTPLIVMAV
                     GAAMLVASGGMVISAPWIGAVAIELVLGAAVGVVIGLMAHLRPSPDHHGVEARNGIGG
                     LAVWLGLIVARIGLGVAFAALGIVGLQSPSTILILLGVNRLARSVVMLSRARAFAVSQ
                     ADAAPARAGH"
     gene            1240..2625
                     /locus_tag="VN041_00560"
     CDS             1240..2625
                     /locus_tag="VN041_00560"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWU27571.1"
                     /translation="MAEQSRLDKVIALARHRGFVFQAGEIYGGSRSAWDYGPLGTELK
                     ENIRRQWWQTFVRGRGDMVGLDSSIILPKRVWEASGHVATFTDPLVECLSCHKRFRAD
                     NLIEDFEARKGRKAENGLADIPCPNCGNKGQYTEPKAFSGLVKTYLGVVDDESGLHYM
                     RPETAQGIFVNFSNVLTASRKKPPFGIGQVGKAFRNEITPGNFIFRTREFEQMEIEYF
                     VPPAEAQEWFEHWVEACWNWFIDLGIDPENIRQFDVPKEDRAHYSAGTIDVEYRFGFA
                     GKEWGELMGVANRTDYDLSSHTEASGQSLTYFDQASGERYTPYVIEPSFGLTRAMMAF
                     LVDAYQEEEVPNAKGGTDTRTVLKLDPRLAPIKVAVLPLSRNERLSPLARSVADGLRG
                     QWTVDFDDAGAIGRRYRRQDEVGTPFCVTVDFDSLDDNAVTVRDRDTMGQERVSLDDL
                     HAYLAERLRGA"
     gene            2972..3691
                     /locus_tag="VN041_00565"
     CDS             2972..3691
                     /locus_tag="VN041_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27572.1"
                     /translation="MTRAPASMMRPAGLVSALSVLVAWMLVLVEPSDYDGASGLLMLF
                     GVPALCVAVLCIVVATRTGMSGAFGGDRCWLWWPLCVMPVGVLAALAVSVLRSPEYFI
                     GEDSPWMLLWMPFLLAAAMLLGTLVAAFWLGPVVALWITTAAIIRGRAKPSAIVMPLV
                     WLAVGVLILIAAASIHAEGVGRMAYGNLILAFFGLPGAYTITWMPGLWIVRAMVLGLV
                     LIFTVPGWRAQRAERAGVAGR"
     gene            complement(3695..4366)
                     /locus_tag="VN041_00570"
     CDS             complement(3695..4366)
                     /locus_tag="VN041_00570"
                     /inference="COORDINATES: protein motif:HMM:NF018952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HWU27573.1"
                     /translation="MSGLALITIGQAPRVDVTPDIAAALIGIEYAEYGALDLLSAHEI
                     EGLRPQEGESVLVSRLRDGGSAVMSERRALPLVQDAVTRAARDGAEAILVMCTGDLPG
                     LHADVPVYLAERLARGGADALAGDDRLAVLVPKREQSSAVAGHWLHDHGRDVGTGVAD
                     PYSAAPGAFAAAAKPLIADGARWIFLDCIGYSEAMADEIRALGVRVLTARTMAARLVA
                     EVASR"
     gene            complement(4366..5226)
                     /locus_tag="VN041_00575"
     CDS             complement(4366..5226)
                     /locus_tag="VN041_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27574.1"
                     /translation="MTALLQARDIVKEFRRRGTKRSAEPFRAVDGVSFDLEEGATLAI
                     VGESGSGKSTTGRCALRLIEPTSGTVTFEGTELTSLSASEMRRRRSRMQIVFQDTYAS
                     LDPRWTVGRLLAEPLRLHERTDRREETRRVGEMLERVGLQASHAGRYPHEFSGGQRQR
                     IGIARALMLRPKLIVCDEPVSALDVSVQAQVLNLMKDLQDELGLSYLFISHDISVVAF
                     MADDMIVMNKGAVVESGACADIIANPADPYTRELLSSVPIADPKVYADRRTARLRASA
                     DAVARQTEAE"
     gene            complement(5223..6062)
                     /locus_tag="VN041_00580"
     CDS             complement(5223..6062)
                     /locus_tag="VN041_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27575.1"
                     /translation="MDTLLSIEGLTVEVRSRRDTVTPVAGIDFDIARGETVGLVGESG
                     SGKSLTAMSIIRLLPTRAVQMTAGRIVFDGVDLATLREAGMRMLRGKRIGTIFQEPMT
                     ALNPAFTVGFQIAEPLRKHLKLNGRQARTRTLELLEMVGIHRPAEVASSYPHHLSGGM
                     RQRAMIAMAMSCEPALLIADEPTTALDVTTQAQILSLISDLQRDHGTAVLLVTHDLGV
                     VAQACDRVVVMRRGEVLERGDVNDIFDRPQHPYTRALLDSMPSRNVGKDRLPYISEGV
                     LAG"
     gene            complement(6075..7013)
                     /locus_tag="VN041_00585"
     CDS             complement(6075..7013)
                     /locus_tag="VN041_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HWU27576.1"
                     /translation="MLRHVMDVVDILDSPHTSGEAVCAYLRSQAVTDADIRYETITGE
                     NGLSTDFVQIVIPGSEGRRSGGDAPTLGIIGRLGGAGARPERIGFVSDGDGAAAALSA
                     GAKLLQMAARGDVLSGDVIVSTHVTGWAPTEPHHPVPFMGSPVDMSVMNEHEVHAEMD
                     AILSIDTTKGNRTINHRGITISPTVKQGYILPPSEDLVGVLETVTGDRAVIFPLATQD
                     ITPYGNGLHHLNSILQPAVATSSPVVGVAITTVVPVAGCATGASHEVDIELAARFAVE
                     TAKYYGKKEISFHDADEFARIVELYGSATALQTQGS"
     gene            complement(7037..8314)
                     /locus_tag="VN041_00590"
     CDS             complement(7037..8314)
                     /locus_tag="VN041_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWU27577.1"
                     /translation="MTNADWEQTLLQAIDDTADELLELAGLLIRTPSENPDGDCTQVA
                     GVIADHLQDVGVSSELLDAGQGRVSVLAHRGESAEGERHLVFAGHHDVVPVGDTSRWS
                     FPPFAGDVVDGWLRGRGASDMKAGLAGLIHVFGLIHRLNVPLAGRISLLSVPDEETGG
                     RLGADWALDQGVLDGATGGVIAEPAERTHPTIGQKGSNWFRLTIQGRPGHGSLQPLHG
                     TSANLLGARAILALQRLWEMTPDQPAELRQLVADSKTYAEEREGYGPGIGDVFDHVTI
                     NVGTIHGGTSTNVVADTCVIEFDTRVPIGLSREQVLARVREILDEEGIPAEIEPLGFT
                     SEPNWTAPTDPVVETLVGALRDLADPDAQGVLQWASSDARTFRSHGIPVLQYGPADLH
                     TIHGFDERARADDVVLAAKVYALTALRYLGTTH"
     gene            complement(8351..9937)
                     /locus_tag="VN041_00595"
     CDS             complement(8351..9937)
                     /locus_tag="VN041_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU27578.1"
                     /translation="MRSTKVFAALAIAAGVALVASGCVPNANSNANSGGKDAASQSIT
                     IAYSEGGQTLDPAEANDGTSDTLVVSAYDQLVTYGTKRVDGKRVSDTTKIEPMIATKW
                     TANADKTAYTFELRDDVKFQDGKALTAEDVVKSFDHIKASASASFLYQMAGISSVEAQ
                     DDHTVVITLTGPNHLFLQILPMYSFSIIDMDQVDKNGGAKWLATNTAGSGPYKIDSWD
                     PANSAKLTANPDYWGDKPAITTVNLKFIDDASNRLQLVKKGSVDMALELDPKDVVSLD
                     GKDGLIVDSRPSNKVLFFAMNTAIKPFDNEKVRQAITYAIPYKKLLTDVMHEQASPMR
                     SAVPSSMPGFTDEGNSAEYNLEKAKQLLAEAGYPKGFTFDFTLGSGFPDWNDDAVLIQ
                     AELAKIGVTMNIHNMARAQFLDALAGKKVQSYISRWTSFVNDPGYHLGLLLTSKGSSD
                     YNNYSNAQVDQLWDEAAKTSDDAARTKLYEQMQQIINEESPWAYLYEYNIVVTHRDDV
                     QGYTSYPDALIRFFQLSKTK"
     gene            complement(10005..10901)
                     /locus_tag="VN041_00600"
     CDS             complement(10005..10901)
                     /locus_tag="VN041_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27579.1"
                     /translation="MPETTTITVLTREAKSPRAVNAFTLLIRNPSAMVGAAIVLLWTV
                     GGLVTLVWSPYPPNLTGAGPLLAAPSPEHLFGTDNYGRDLFSRVLAGARVSLWTGVIA
                     VSLSVVIGVPVGAIAGYVRGATGMVLMRLMDMLLAFPSLLLAMALAVSFGPGLGSAMI
                     AVGIVGIPEFARIAYGQVTSLRQREFVEASHAIGLVDRVILFRHILPNGVVPIIVRCA
                     LGMGYAILTAAALSFIGLGAQPPLAEWGVMISDARGYIVSGQWWMTVFPGIAIASSIL
                     GFNLFGDGLRDVLDPRLRTSGR"
     gene            complement(10894..11901)
                     /locus_tag="VN041_00605"
     CDS             complement(10894..11901)
                     /locus_tag="VN041_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27580.1"
                     /translation="MKTTLRRVLAMIPTLLGVIVIIFLITRVLPGDPARTIAGENAAP
                     STVAKLRQQMGLDEPIWQQFLNYFLGLFRGDLGFAWHTGHPVAQDFASRFPATVELAL
                     AALLIGILIGVPLGIVGAVRRGLVSDHATRVISLLGISMPLFWLGLLVISFFYANLGW
                     EPAPIGRIGDTVNPPTHVTGLYVLDSIITGDSVALGSSLSHLIWPAMVLSVGTIAIVS
                     RMTRSSMLEVLGQDYIATAKAKGMGPVKVVGAHVLKNAAPTIVTVIGLELGQLLGGAV
                     ITETIFSWPGIGNYVVQSIEATDYAPVQAMTLAAAVIYLIVNLLVDLSQAVFDPKVRD
                     A"
     gene            12058..12864
                     /locus_tag="VN041_00610"
     CDS             12058..12864
                     /locus_tag="VN041_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWU27581.1"
                     /translation="MSSASKSQSAGSPLQTVDRALAVLLSFTARRSEWGVTELAEEFG
                     FDKSSAQRLLAALAARGFLSVDEITHRYRLGPAVWRIASSWERRGGMAMLVNPLLAEL
                     ADATMRTAVFTLADGAYLRCVAAVDGGHVSMRDHPLVGEVYPAHAGATSRAYFAFLPK
                     GQRESLIYRGPLARFSKLTELDPVAIEAEMAKTSEAGWAYSEGEYDVNTRAVAAPVMV
                     GAQPIGSVSVGERKNDPVDDILDHVEAVQETAKKIGMLLSHPTHRKPSGT"
     gene            complement(12887..13639)
                     /locus_tag="VN041_00615"
     CDS             complement(12887..13639)
                     /locus_tag="VN041_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWU27582.1"
                     /translation="MTERLSSVGRALETLLLFDANRHSLSVTELAELLGCSTSTAQRT
                     IATLTEQQFLRRDDITRTYRLGASVMQLARAWRSTASLASIARPVLERLAESTGFNAT
                     FAVPDHAHMRVVLSIDGAEGPLRDYPMASELYPAHAGAISKAYYAQLPHALRARFIGD
                     RPMARFTAETKTDPQQLEEQFLAIRAHGYCYSQGEYDTHVSALAAPVWMGKEPLGAVA
                     LSSLSTLMVEQPLVDALLAATGELGRLLTRES"
     gene            13733..14914
                     /locus_tag="VN041_00620"
     CDS             13733..14914
                     /locus_tag="VN041_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU27583.1"
                     /translation="MALDDQAPHASQQYAYFDTAAAAPPVPEAVQAAVEYLQRTAVDG
                     PYLPSFRREVYEKLEQIRASVAGFLEAAPEEIAFTRNGTESISLVARGIRWQPGDEVI
                     VPDTEMLSNIAIWRMLESDAGIRVVPFAADPDGMLDPERLRAAITPRTRLITFVALSN
                     VTGAVQPVAEICRIAADAGVLTHVNAAQAIGMMPLGFRTWQADFISACSRKGLRGIEG
                     SGVLAARKHLIPGLTPALAGWWNTGLDAGGITLPDTAKRFEAGGPNVPAILALGAAVE
                     VAERRGIDSIHRGVRDLTDAAVRGIRSIPGAAIYGPSDENRLGIIPFNISGVDPRELT
                     DHLESRGIIIEAGHFMATPILNAYGISAMARASVHYFNTIDEIDRLVAEITAFAGKEA
                     A"
     gene            14911..15654
                     /gene="pepE"
                     /locus_tag="VN041_00625"
     CDS             14911..15654
                     /gene="pepE"
                     /locus_tag="VN041_00625"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HWU27584.1"
                     /translation="MIRALLMSSSRKDSLGYLEHAHDQLGLLLGEQEDRAKPTRAVLI
                     PYAAVSTSYEKYEELAKPAFAAHDVELTSIHRMDDPVQAVREAEVIAMAGGNTFALLS
                     RLYEAGLVDEIRERVASGAAAYVGWSAGTNVATPSIRTTNDMPIVEPPSFRALRLVPF
                     QMNPHFIPGKPVGHNGESREERLGEFTDLNPTEEVLALYEGCALWVTGDQARIVGGRE
                     GLVFSHGADVRSIAPEETFALSSIRGAAL"
     gene            15654..>15923
                     /locus_tag="VN041_00630"
     CDS             15654..>15923
                     /locus_tag="VN041_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 family protein"
                     /protein_id="HWU27585.1"
                     /translation="MGMREIAEVTELMDDPRLDGERIAVLLRSAGAADARIEITPIPY
                     EAPEDTSRTCDFVRVVLPGTRGALAGGHAPTLGIVGRLGAQQAQPD"
BASE COUNT         2500 a         5366 c         5519 g         2538 t
ORIGIN      
        1 gctcctgcgc cgtgcgccgc tctcgctcgg tggcctggcg tgcctgccgc cgggcaaggc
       61 cgatcagaac gccggccgcg atcccggcga tcgacgccag cacctgcagc atgggaggtg
      121 cggtcagagc gcagaaagcg atggccagca ggccggatgc cacgaatgcg aaccccaccg
      181 cgagcgacag caccgtcgac ccgatcacgg tgacgatccc gacgatcagc accgccgagg
      241 cgacgcccgt ggtggtggtc gaggccacgg cgcccccgat caccatcagc gccagcagca
      301 cgagccgcaa ccagagcgag gcgcccgccg gcagcgcggc cagaatcagc cacgccacga
      361 gcgccgtgac ggcgattacc gtcatccagg gagacgagga gccggtgtca cgccacagca
      421 cgaacgcgat cagcgcgatc ccgagcaggg agatcaggcg cccgacgccg cgcggtccgg
      481 ggactcgagc cggcatcggg acgtcagcgg gggcgctcac ctcgaatccc gcgtgcgcca
      541 tgcgcccatc attccgcatg cctcccgcga ctgcaccttc tcagtgaccc gctcgcgcag
      601 gagcggcatc cgcctggctg acggcgaacg cgcgagcgcg ggagagcatg accaccgagc
      661 gggcgagccg gttcacgccg agcaggatca ggatcgtgct cggggactgc agcccgacga
      721 ttccgagggc ggcgaacgcg acgcccaggc cgatccgcgc gacgatcagc cccagccaca
      781 cggccagtcc gccgatgccg ttcctcgcct cgaccccgtg gtggtccggg gaggggcgca
      841 ggtgcgccat gaggccgatc acgactccca cggcggcgcc gagcacgagc tcgatcgcga
      901 cggccccgat ccagggggcg ctgatcacca ttccacctga ggccaccagc atcgcggcgc
      961 cgaccgccat cacgatcagc ggtgtgcgaa acgcacggcc cggatcgagc gtccgccagg
     1021 tggtctgacg gatgctcagc cagacgatca cggcaagcga gatgaggatg gtggtgagaa
     1081 cgctgatgtc catgcctcca ctcttcgccg tggccgggcg gaggcggagg gccggcgggt
     1141 ggagatcggg tggagggcag ggttgaagag agccgcggat agaatgagga tgcccgtgat
     1201 cacgggcgtt tcccgcagca tgcgaccatt ggagccaccg tggccgaaca gtcccgtctt
     1261 gacaaggtca tcgccctcgc ccgccaccgc gggttcgtgt tccaagcggg tgagatctac
     1321 ggcggttccc gttcagcatg ggactacggc cccctcggca ccgagctgaa ggagaacatc
     1381 cgccggcagt ggtggcagac cttcgtgcgc ggccgcggcg acatggtggg tctggactcg
     1441 tcgatcatcc ttcccaagcg cgtgtgggag gcgtccggtc acgtcgcgac ctttaccgac
     1501 ccgctggtgg agtgcctcag ctgccacaag cgcttccgtg ccgacaacct catcgaggac
     1561 ttcgaggcgc gcaagggccg caaggccgag aacggcttgg ccgacatccc gtgccccaac
     1621 tgcggcaaca agggtcagta caccgagccc aaggcgttct cgggtctcgt gaagacctac
     1681 ctcggcgtcg tcgacgacga atcgggtctg cactacatgc gccccgagac cgctcagggc
     1741 atcttcgtga acttctcgaa cgtcctcacc gcctcgcgca agaagccgcc gttcggcatc
     1801 ggacaggtcg gcaaggcgtt ccgcaacgag atcacccccg gcaacttcat cttccgcacc
     1861 cgcgagttcg agcagatgga gatcgagtac ttcgtgccgc cggccgaggc gcaggagtgg
     1921 ttcgagcact gggtcgaggc ctgctggaac tggttcatcg acctcggcat cgacccggag
     1981 aacatccgcc agttcgacgt gccgaaggag gaccgcgcgc actactccgc cggcaccatc
     2041 gacgtcgagt accggttcgg cttcgcgggc aaggagtggg gcgagctgat gggcgtcgcc
     2101 aatcgcaccg actacgacct gtccagccac accgaggcgt ccggccagag cctgacgtac
     2161 ttcgaccagg catccggcga gcggtacacc ccgtacgtga tcgagccgtc gttcggtctc
     2221 acccgcgcga tgatggcgtt cctcgtcgac gcgtaccagg aggaggaggt gccgaacgcg
     2281 aagggcggca ccgacacccg caccgtgctg aagctcgacc cgcgtctggc gccgatcaag
     2341 gtcgccgtgc tgccgctgtc gcgcaacgag cgcctctcgc cgctggcccg ctcggtggct
     2401 gacggcctgc gcggtcagtg gaccgtggac ttcgacgacg ccggcgccat cggccgccgc
     2461 taccgtcgtc aggacgaggt cggaaccccg ttctgcgtca cggtcgactt cgactcgctc
     2521 gacgacaatg ccgtgacggt gcgcgaccgc gacaccatgg gccaggagcg cgtgtcgctg
     2581 gacgacctgc acgcctacct cgccgagcgc ctgcgcggcg cctgagatcg gacacacgaa
     2641 ggccccgatt ccccccgcgg gaatcggggc cttcgtctgc gctcgtcagt cgagcgcgcg
     2701 gaggcgagag ctcagaagtc ctcgccctcg gcctgcgagg ggtgcttgtc gaaccacagc
     2761 gggcggccga tgtgcagcag ggcgtcgctg tcgaagaggt cgtgggtgtc ggtgacgttc
     2821 atgagtgctc ctagcgtggg ggtctcaatc cagcatccga tagagaatcg tgctcttgcg
     2881 agggaattct cgcgaaagaa gcgcgaatcc ttcaccttgg gcaaggaccg tgccgcgcta
     2941 gggtgatcgg gtgggggaat cggatgctga ggtgacgcgc gcacctgcgt cgatgatgcg
     3001 cccggcgggg ctggtctccg cgctgtccgt gctggtcgcg tggatgctcg tgctcgtcga
     3061 gccgagcgac tacgacggag cctccggtct gctgatgctg ttcggggtgc cggcgctgtg
     3121 cgtggcggtg ctgtgcatcg tcgtggcgac gcggaccggc atgagcgggg cgttcggcgg
     3181 cgaccggtgc tggctgtggt ggccgctgtg cgtcatgccg gtcggcgtgc tcgccgcgct
     3241 ggcggtgagc gtgctgcgca gccccgagta cttcatcggc gaggactcgc cgtggatgct
     3301 gctgtggatg ccgttcctcc tggccgcggc gatgcttctc ggcaccctgg tggcggcgtt
     3361 ctggctgggc ccggtcgtcg cgctgtggat cacgacggcg gcgatcatcc gcggccgggc
     3421 gaagccgagc gccatcgtca tgccgctggt gtggctggcc gtcggcgtgc tgatcctgat
     3481 cgccgcggca tccatccacg ccgagggcgt cgggcggatg gcctacggca acctgatcct
     3541 ggcattcttc ggcctgcccg gcgcgtacac gatcacctgg atgccgggcc tgtggatcgt
     3601 gcgcgcgatg gtgctcgggc tggtgctgat cttcaccgtc ccaggatggc gcgcacagcg
     3661 ggccgagcgg gctggcgtgg ccggccgctg agcctcagcg gctcgcgacc tcggcgacca
     3721 ggcgcgccgc catcgtccgc gcggtgagca ctcgcacgcc gagtgcgcgg atctcatccg
     3781 ccatcgcctc ggagtagccg atgcagtcca ggaagatcca ccgtgccccg tccgcgatca
     3841 gtggcttcgc ggctgcggcg aacgcgcccg gcgccgcgga gtacgggtcg gcgacaccgg
     3901 taccgacatc gcgtccgtgg tcgtgcagcc agtgtcctgc cacggcggag gactgctcgc
     3961 gcttgggcac cagaacggcc aggcgatcat cgccggcgag agcatcggca ccaccgcggg
     4021 cgagccgctc ggcgaggtag accggcacgt cggcatgcag cccgggcaga tcgcccgtgc
     4081 acatcaccag gatggcttcg gccccgtccc gggcagccct ggtcacggca tcctgcacga
     4141 ggggcagcgc ccgccgctcc gacatgaccg ccgagccccc gtcgcgcaga cgtgagacga
     4201 gcacggactc gccctcctgc ggccgaagcc cctcgatctc gtgcgcggag aggagatcga
     4261 gcgccccgta ctcggcgtat tcgatgccga tcagagctgc ggcgatgtcg ggcgtgacgt
     4321 ccacacgcgg cgcctgcccg atggtgatga gggccagtcc gctcatcact cggcctccgt
     4381 ctgccgggcg acggcatccg cggatgccct gaggcgggcc gtgcgccgat cggcgtagac
     4441 cttcgggtcc gcgatgggaa cggaggagag cagttcgcgt gtgtacggat ccgccgggtt
     4501 cgcgatgatg tccgcacacg cgccggactc gaccaccgcg cccttgttca tcacgatcat
     4561 gtcgtcggcc atgaacgcca ccaccgagat gtcatgcgag atgaacaggt agctcagccc
     4621 cagttcatcc tgcagatcct tcatcaggtt cagcacctgc gcctgcacgg agacgtccag
     4681 cgccgacacc ggctcgtcgc acacgatcag cttggggcgc agcatgagcg cccgggcgat
     4741 gccgatacgc tgccgctgcc caccggagaa ctcgtgcggg tagcgtccgg cgtgcgatgc
     4801 ctgaagcccg acgcgctcga gcatctcccc gacacggcgc gtctcctcac ggcgatcggt
     4861 ccgctcgtgc agccgcagcg gctcggcgag cagccgaccg acggtccatc tcggatccag
     4921 cgaggcgtag gtgtcctgga agacgatctg catgcgcgat ctgcgccgac gcatctcgga
     4981 cgccgacagg gacgtcagct cggtgccttc gaaggtcacg gtgcccgagg tcggctcgat
     5041 cagacgcagc gcgcagcggc cggtcgtcga ctttcccgac cccgactcac ccacgatcgc
     5101 cagcgtcgca ccctcttcga ggtcgaagct cacgccatcg acggcgcgga acggctcagc
     5161 cgatcgcttc gtcccgcgcc ggcggaactc cttcacgatg tcgcgggcct gcagcagtgc
     5221 ggtcatcctg cgagcacccc ctccgagatg tagggaagcc ggtccttgcc gacgttacgc
     5281 gacggcatgg agtccagcag cgcacgtgtg tacggatgct ggggccgatc gaagatgtcg
     5341 ttcacatcac cgcgctccag cacttcgccg cgccgcatga ccaccacgcg gtcgcacgcc
     5401 tgggcgacca ctcccaggtc gtgcgtgacc agcagcacgg cggtgccgtg gtcgcgctgc
     5461 agatcgctga tcagtgacag gatctgcgcc tgcgtggtga cgtcgagcgc tgtggtgggc
     5521 tcgtcggcga tcaacagggc gggctcgcag gacatcgcca tggcgatcat cgcgcgctgc
     5581 ctcatgccgc cggagaggtg gtgcgggtag gagctcgcga cctcggcggg acggtggatg
     5641 ccgaccatct ccagcagctc gagcgtgcgc gtgcgcgcct gccgtccgtt cagcttcagg
     5701 tgcttgcgga gcggctcggc gatctggaac ccgaccgtga acgcaggatt gagcgccgtc
     5761 atgggctcct ggaagatcgt gccgatgcgc ttgccgcgca gcatccgcat tcccgcctcc
     5821 ctgagtgtgg ccaggtcgac gccgtcgaac acgatccgcc ctgcggtcat ctgcacggcg
     5881 cgcgtgggca gcaggcggat gatggacatc gccgtgaggc tcttgccgga tccggactcg
     5941 ccgacgagtc cgacggtctc gccccgcgcg atgtcgaaat cgatccccgc gaccggggtg
     6001 accgtgtcgc ggcgtgagcg gacctcgacg gtcagcccct cgatgctcag cagcgtgtcc
     6061 atcagtcgct ccgcctacga cccctgcgtc tgcagggcag tcgcggatcc gtacagttcg
     6121 acgattcgcg cgaactcatc ggcatcatgg aagctgatct ccttcttgcc gtagtacttc
     6181 gcggtctcga cggcgaatcg ggctgccagc tcgatgtcga cctcgtggct ggcgccggtg
     6241 gcgcatccgg cgaccgggac gaccgtggtg atggccacgc cgacgacggg ggaggaggtc
     6301 gcgacggccg gctgcaggat cgagttgagg tggtgcaggc cgttgccgta gggcgtgatg
     6361 tcctgggtgg ccagcgggaa gatcacggcg cggtcgcccg tcaccgtctc cagcacgccg
     6421 acgagatcct cgctcggcgg caggatgtag ccctgcttca cggtcggaga gatcgtgatg
     6481 ccgcgatggt tgatcgtgcg gttgcccttg gtcgtgtcga tcgagaggat ggcgtccatc
     6541 tcggcgtgga cctcgtgctc gttcatcacg ctcatgtcca caggtgagcc catgaacggc
     6601 accgggtggt ggggctcggt gggtgcccag cccgtgacat gggtggagac gatcacatca
     6661 ccggagagca catcccctcg tgccgccatc tgcagcagct tcgctccggc cgacaatgcg
     6721 gctgccgcgc cgtcgccgtc ggagacgaag ccgatccgct ccggccgtgc gccggcgccg
     6781 cccaggcgtc cgatgatgcc gagcgtgggg gcgtcgcctc cactgcgcct gccctcggag
     6841 ccggggatca cgatctgcac gaaatcggtc gacaggccgt tctcgccggt gatggtctcg
     6901 taccggatgt cggcgtcggt cacggcctgg ctgcgcaggt acgcgcagac cgcctcgccg
     6961 gaggtgtggg gggagtcgag gatgtcgacg acatccataa cgtgacggag catgtcttct
     7021 cttctcggtt cggggatcag tgcgtggtgc ccaggtagcg cagcgccgtg agcgcgtaca
     7081 ccttggcggc caggacgacg tcgtcggcgc gggcgcgttc gtcgaagccg tggatcgtgt
     7141 gcaggtcggc cggtccgtac tggagcaccg ggatgccgtg actgcggaag gttcgggcat
     7201 ccgaggacgc ccactgcagg acgccctgcg cgtcggggtc ggcgaggtcg cgcagcgcac
     7261 cgaccagggt ctcgacgacg gggtcggtgg gagccgtcca gttcggctcg ctcgtgaagc
     7321 cgagcggctc gatctcggcg gggatgccct cctcgtcgag gatctcccgc acccgcgcga
     7381 gcacctgctc gcggctgagc ccgatcggga cacgcgtgtc gaactcgatg acgcaggtgt
     7441 cggcgacgac gttggtggag gtgccgccgt ggatcgtccc gacgttgatc gtgacgtggt
     7501 cgaacacgtc tccgatcccg ggaccgtatc cctcccgctc ctctgcgtag gtcttggagt
     7561 ccgcgaccag ctgccggagc tcggcgggct ggtcgggggt catctcccag agacgctgca
     7621 gggcgaggat cgcgcgtgcg ccgagcaggt tcgcactggt gccgtgcagc ggctggaggc
     7681 tgccgtggcc gggccgaccc tggatcgtca gccggaacca gttgctgccc ttctgcccga
     7741 tcgtcgggtg cgtgcgctcg gccggctcgg cgatgactcc gccggtggcg ccgtcgagga
     7801 caccctggtc cagcgcccag tcggcgccca gacgtccgcc ggtctcctca tcgggcacgg
     7861 agagcaggga gatccggccc gcgagcggaa cgttcagtcg gtggatcagg ccgaacacgt
     7921 ggatgagccc ggcgaggccg gccttcatgt cgctggctcc gcggccgcgc agccagccgt
     7981 ccacgacgtc gccggcgaac ggagggaacg accagcggga cgtgtcgccg acggggacca
     8041 cgtcgtggtg gccggcgaag accaggtgcc gctcgccctc cgcggactcg ccgcgatgcg
     8101 cgagcacgct cacgcgcccc tgaccggcat ccagaagctc cgatgacact ccgacgtcct
     8161 ggagatggtc ggcgatcaca ccggccacct gcgtgcagtc cccgtccgga ttctcgctcg
     8221 gagtgcggat cagcagaccc gccagctcga gcagctcgtc ggcggtgtcg tcgatcgcct
     8281 gcagcagcgt ctgctcccag tcggcgttcg tcatcgtcgt ccttctttcg tggtcagggg
     8341 cgcgtgcgcg tcacttggtc ttgctgagct ggaagaagcg gatcagggcg tccgggtagg
     8401 acgtgtatcc ctgcacgtca tcccggtgcg tgaccacgat gttgtactcg tacaggtacg
     8461 cccacggcga ctcctcgttg atgatctgct gcatctgctc gtacagcttc gtgcgagccg
     8521 cgtcgtcact cgtcttcgcg gcctcgtccc acagctggtc gacctgcgcg ttggagtagt
     8581 tgttgtagtc gctggagccc ttgctggtca gcagcagccc caggtggtac ccggggtcgt
     8641 tgacgaagga cgtccagcgc gagatgtacg actgcacctt cttgccggcg agggcgtcga
     8701 ggaactgcgc gcgggccatg ttgtggatgt tcatcgtcac gccgatcttg gccagttcgg
     8761 cctggatcag tacggcgtcg tcattccagt ccgggaagcc cgagccgagg gtgaagtcga
     8821 acgtgaagcc cttcgggtac ccggcctcgg cgagcagctg cttggccttc tcgaggttgt
     8881 actcggcgga gttgccctcg tcggtgaagc cgggcatcga gctcgggacg gcggagcgca
     8941 tggggctcgc ctgttcgtgc atgacgtcgg tgaggagctt cttgtagggg atcgcgtagg
     9001 tgatggcctg gcggaccttc tcgttgtcga acggcttgat ggcggtgttc atcgcgaaga
     9061 acagcacctt gttgctcggc ctcgagtcca cgatcagacc gtccttgccg tcgaggctca
     9121 cgacgtcctt cgggtccagc tcgagcgcca tgtcgaccga gcccttcttg accagctgga
     9181 ggcggttcga cgcatcatcg atgaacttga ggttcacggt cgtgatcgcc ggcttgtcgc
     9241 cccagtagtc aggattggcg gtcagcttgg ccgagttcgc cgggtcccag ctgtcgatct
     9301 tgtatgggcc gctgcctgcc gtgttggtcg ccagccactt ggcaccgccg ttcttgtcga
     9361 cctgatccat gtcgatgatc gagaacgagt acatcggcag gatctgcagg aacaggtgat
     9421 tcgggccggt cagcgtgatg accaccgtgt ggtcgtcctg tgcctcgacg gacgagatgc
     9481 ccgccatctg gtagaggaag ctcgccgatg cggaggcctt gatgtgatcg aagctcttca
     9541 ccacgtcctc tgccgtgagc gccttgccgt cctggaactt gacgtcgtcg cgcagctcga
     9601 acgtgtaggc ggtcttgtcg gcgttggccg tccacttcgt ggcgatcatc ggctcgatct
     9661 tggtggtgtc ggagacgcgc ttgccgtcga ctcgcttggt cccgtacgtg acgagctggt
     9721 cgtacgccga cacgacgagc gtgtcgctgg tgccgtcgtt ggcctctgcc gggtcgagcg
     9781 tctgcccgcc ctcggagtag gcgatggtga tgctctgcga tgcggcgtcc ttgccgccgg
     9841 agttggcgtt gctgttcgcg ttcgggacgc agcccgaggc gaccagcgcc acgccggcag
     9901 cgatcgcgag tgcagcgaag accttcgtgc ttctcatcgt cgtgatgcct ttcatgtgat
     9961 gtgttcgggt gagggaaaga tgccttgtgg gcggggacgg gggctcagcg tccggaggtg
    10021 cgcagccgcg gatcgagtac gtcgcgcagt ccgtcgccga agaggttgaa gccgaggatg
    10081 gaactggcga tcgcgatgcc ggggaagacc gtcatccacc actgccccga cacgatgtag
    10141 ccacgggcat ccgagatcat gacgccccac tcggccagcg gcggctgtgc gcccaggccg
    10201 atgaagctca gcgcggcggc ggtgaggatc gcatagccca tgcccagcgc gcagcgcacg
    10261 atgatgggga cgacgccgtt gggcaggatg tgccggaaca ggatgacccg gtcgacgagc
    10321 ccgatcgcgt gcgaggcctc gacgaactcg cgctgccgga gcgacgtgac ctgcccgtag
    10381 gcgatccggg cgaactcggg gatgccgacg atgcccaccg cgatcatggc cgagccgagt
    10441 ccgggaccga aggagaccgc cagggccatc gccagcagca gcgaggggaa cgcgagcagc
    10501 atgtccatca ggcgcatcag caccatgccg gtggcgccgc gcacgtaccc cgcgatcgcg
    10561 ccgacgggaa cgccgatgac gaccgagagg gagacggcga tcacgcccgt ccacagggac
    10621 acccgggcgc ccgcgagcac ccgcgagaac aggtcccgac cgtagttgtc ggtgccgaac
    10681 aggtgctccg gcgacggagc ggcgagcagc ggtcccgcgc ccgtcaggtt gggcgggtaa
    10741 ggcgaccaga cgagggtgac gaggccgccg accgtccaga gcaggacgat cgcggcgccc
    10801 accatggcgg acggattgcg gatcaggagc gtgaacgcgt tcacggcacg gggcgacttc
    10861 gcctcgcggg tgagcacggt gatggtcgtc gtctcaggca tcgcgcacct tcgggtcgaa
    10921 gacggcttgg gacaggtcca caagcaggtt gacgatgagg tagatcacgg cggcggccag
    10981 ggtcatcgcc tgcaccggcg cgtagtcggt ggcctcgatc gactgcacga cgtagttgcc
    11041 gatgccgggc cagctgaaga tggtctcggt gatgaccgcg ccgcccagca gctggccgag
    11101 ctcgaggccg atgaccgtga cgatcgtggg ggccgcgttc ttgagcacgt gggcgccgac
    11161 gaccttgacc ggccccatcc ccttcgcctt cgccgtggcg atgtagtcct gcccgaggac
    11221 ctcgagcatc gaggagcggg tcatccggga gacgatcgcg atcgtcccga ccgagagcac
    11281 catggcgggc cagatcaggt gcgacaggga gctgccgagg gcgacggagt cgccggtgat
    11341 gatgctgtcg agcacgtaga gacccgtgac gtgcgtgggc gggttcacgg tgtcgccgat
    11401 gcgcccgatc ggcgccggct cccagccgag gttggcgtag aagaacgaga tgaccaggag
    11461 cccgagccag aacagcggca tcgagatccc cagcagcgag atcacgcggg tcgcgtggtc
    11521 gctcaccagg ccccgccgca cggcgccgac gatgccgagc ggaacgccga tcaggatgcc
    11581 gatgagcagg gccgcgagtg cgagctcgac ggtggcgggg aagcggctcg cgaagtcctg
    11641 cgcgaccgga tggccggtgt gccaggcgaa tccgagatcg ccgcggaaca gtcccaggaa
    11701 gtagttcagg aactgctgcc agatgggctc gtcgaggccc atctgctgcc ggagcttggc
    11761 gaccgtgctc ggtgcggcgt tctcgccggc gatcgtgcga gcgggatctc ccggcagcac
    11821 gcgcgtgatc aggaagatga tcacgatgac gccgagcaga gtcgggatca tcgccagcac
    11881 gcggcgaagc gtcgtcttca cttctcctcc tgcgcggtca tcggatgggg gctgtcttcg
    11941 tcgacatctg agaactatcg ccctgcgccc gagccgcacg caatgacttc ccgttatatg
    12001 caacgctggt gcattcttgg acgaacgcca gtacgtttgt gtcgtcaaag gagagctatg
    12061 agttccgcat cgaaatcaca gagtgccggg tcgcccctgc agaccgtgga tcgcgccctc
    12121 gccgtgctgc tctcgttcac ggcccggcgg tcggagtggg gcgtcaccga actcgctgag
    12181 gagttcggct tcgacaagtc atcggcgcag cgtctgctgg ccgcgctcgc cgcacgcggg
    12241 ttcctctcgg tcgacgagat cacgcaccgc taccggctgg ggcccgcggt ctggcgcatc
    12301 gcatcgagct gggagcgccg cggaggcatg gcgatgctcg tcaacccgct gctcgcggag
    12361 ctggcggatg ccacgatgcg cacggccgtg ttcaccctgg ccgacggtgc gtatctgcgt
    12421 tgtgtcgcgg ccgtcgacgg cggacacgtc tccatgcgag atcatccgct ggtcggcgag
    12481 gtctaccccg cgcatgcggg tgccacgtcg cgcgcctact tcgcgttcct cccgaagggg
    12541 cagcgcgaat ccctgatcta ccgcggaccc ctggcacggt tcagcaagct gaccgagctg
    12601 gatcccgtcg ccatcgaagc cgagatggcg aagacctcgg aagcagggtg ggcgtactcc
    12661 gagggagagt acgacgtcaa cacgcgtgcg gtcgcagcgc cggtcatggt cggcgcccag
    12721 ccgatcggct ccgtgagcgt cggagagcgg aagaacgacc ccgtggacga catcctggac
    12781 cacgtcgagg ccgtgcagga gacggcgaag aagatcggca tgctgctctc gcacccgact
    12841 catcgcaagc cgtccggcac gtgagcccga gggatcgtcc ccggcctcag ctctcgcgag
    12901 tgagcagtcg cccgagttcc cccgtcgcgg ccagcagcgc gtcgaccagc ggctgctcca
    12961 ccatgagcgt cgacagggac gacagcgcca ccgctccgag cggctccttg cccatccaca
    13021 ccggcgccgc cagggccgac acgtgggtgt cgtactcacc ctgcgaatag cagtagccgt
    13081 gggctctgat cgccaggaac tgctcctcga gctgctgcgg gtccgtcttc gtctcggcgg
    13141 tgaaccgcgc catcggacgg tcgccgatga agcgcgcgcg cagggcgtgc ggcagctgcg
    13201 cgtagtacgc cttcgagatc gcgccggcat gcgcggggta cagctcgctc gccatcggat
    13261 agtcccgcag cggtccttcc gcgccgtcga tggacagcac cacgcgcatg tgcgcgtggt
    13321 ccggcaccgc gaacgtcgcg ttgaacccgg tgctctcggc cagtcgctcc agcacgggcc
    13381 gggcgatgct ggccagggag gccgtgctgc gccacgcacg cgccagctgc atgacggagg
    13441 cgcccagtcg atacgtgcgc gtgatgtcat cgcgccgcag gaactgctgc tcggtgagcg
    13501 tcgcgatggt ccgctgtgct gtcgaggtgg agcacccgag cagctcggcg agctccgtga
    13561 cgctcagcga gtgccggttc gcgtcgaaga gcagcagggt ctccagtgcc cggcccacgc
    13621 tgctcagccg ctccgtcatg aggagattat cccgcatagc gggatgagta ttgtccgcgt
    13681 ttccgcgggg gccgaatctg tgttgagccc cccgacgaag aggaccgaga agatggcact
    13741 ggacgatcag gcaccgcacg cgtctcagca gtacgcgtac ttcgacaccg ccgcggcggc
    13801 accgcctgtg cccgaggccg tgcaggccgc cgtcgagtac ctgcagcgca cggccgtcga
    13861 cggtccgtac ctgcccagct tccggcgcga ggtgtacgag aagctcgagc agatccgggc
    13921 atccgtcgcg ggcttcctcg aggccgcccc ggaggagatc gctttcaccc gcaacggcac
    13981 cgaatccatc agcctggtcg cccggggcat ccgctggcag cccggcgacg aggtgatcgt
    14041 gcccgacacc gagatgctca gcaacatcgc catctggcgg atgctcgagt cggacgccgg
    14101 catccgcgtc gtcccgttcg cggccgatcc cgacgggatg ctcgaccccg agcggctgcg
    14161 cgcggccatc actccgcgca cgcgcctcat cacgttcgtg gcgctctcca acgtgaccgg
    14221 cgccgtgcag ccggtcgccg agatctgccg catcgcggcg gatgccggcg tgctcaccca
    14281 cgtgaacgcc gcccaggcca tcggcatgat gccgctgggc ttccgcacct ggcaggcgga
    14341 cttcatctcc gcgtgcagtc gcaaggggct gcgcggcatc gagggctcgg gcgtgctcgc
    14401 cgcgcgcaag cacctgatcc ccgggctcac ccccgcgctg gccggatggt ggaacaccgg
    14461 cctggatgcc ggcggcatca ccctgcccga caccgcgaag cgcttcgaag ccggcggtcc
    14521 gaacgtgccc gccattctcg cgctcggtgc ggccgtcgag gtcgccgagc gacgcggcat
    14581 cgacagcatc caccgcggtg tccgcgacct cacggatgcc gcagtccgcg gaattcgcag
    14641 catccccggc gccgcgatct acggaccctc cgacgagaac cgcctgggga tcatcccctt
    14701 caacatctcc ggcgtcgacc cccgtgagct caccgaccat ctcgagagcc gcggcatcat
    14761 catcgaggcc ggccacttca tggccacccc gatcctgaac gcctacggga tctccgccat
    14821 ggcacgggca tccgtgcact acttcaacac catcgacgag atcgaccgtc tcgtcgccga
    14881 gatcacggcg ttcgccggaa aggaagcagc atgatccgcg cactcctgat gagcagctcg
    14941 cgcaaggaca gcctcggcta cctggagcac gcacacgacc agctcgggct cctgctcggc
    15001 gagcaggagg atcgcgccaa gccgacccgt gccgtcctga tcccctatgc cgccgtgtcg
    15061 acgtcctatg agaagtacga ggagctcgcg aagcccgcgt tcgcagccca cgatgtggag
    15121 ctcacctcga tccaccgcat ggacgacccg gtgcaggccg tgcgcgaggc cgaggtcatc
    15181 gcaatggccg gcggcaacac gttcgctctg ctgagccgtc tctacgaggc cggactggtg
    15241 gacgagatcc gtgaacgcgt cgcgtcgggc gcagcggcct acgtcggctg gagcgcgggt
    15301 accaacgtgg ccacgccgtc catccgcacc accaatgaca tgccgatcgt ggagcctccg
    15361 tcgttccgcg cgctgcgcct ggtgccgttc cagatgaacc cgcacttcat ccccggaaag
    15421 cccgtcggac acaacggtga gagccgcgag gagcgcctcg gcgagttcac cgacctcaac
    15481 ccgaccgaag aggtactggc gctctacgaa ggctgcgccc tgtgggtcac cggtgaccag
    15541 gcgcgcatcg tgggagggcg cgagggtctc gttttcagcc acggtgcgga tgtgcgcagc
    15601 atcgcgccgg aggagacctt cgcgctgtcg tcgatccgcg gagcggcgct ctgatgggca
    15661 tgcgtgagat cgccgaggtc accgaactca tggacgatcc gcggctggac ggcgagcgca
    15721 tcgccgtcct gctgcgcagc gcaggagctg cggatgcccg gatcgagatc actccgatcc
    15781 cgtacgaggc ccctgaggac accagccgca cctgtgactt cgtgcgcgtc gtgcttcccg
    15841 gcacgcgcgg cgcactcgcc ggtggccacg ccccgacgct cggcatcgtc ggacgcctcg
    15901 gcgcccagca ggcgcagccg gac
//
LOCUS       DATMSX010000019         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_47634,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000019 DATMSX010000000
VERSION     DATMSX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47634"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="dnaG"
                     /locus_tag="VN041_00635"
     CDS             complement(<1..1287)
                     /gene="dnaG"
                     /locus_tag="VN041_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWU27586.1"
                     /translation="MPRILQADVEEVKSRTNIADIVGERVALKSAGVGSMKGLCPFHD
                     EKSPSFHVRPQVGYYHCFGCGASGDVYSFLREMDHVSFTEAVERLAGRIGYPLHYEDG
                     GAAPETSGRSRLYAANSAAAEYFRGQLLSPEAESARRFLGERGFDAGAAAHFGVGYAP
                     KGWENMTKALTAQGFTREELTNAGLVSAGQRGVYDRFRGRVIWPIRDVTGQTIGFGAR
                     RLYDDDKGPKYLNTPETPLYKKTQVLYGLDLAKRDIARGDPRRVVVVEGYTDVMACHL
                     AGVTTAIATCGTAFAAEHIKVLRRVMGDDSAAGEVVFTFDGDEAGQKAALRAFSEDSR
                     FTAQTFVAVAPDGLDPCDLRLQRGDAAVRGLMDAKVPMFEFAIDRKLAGYDLATVEGQ
                     VGALRAAAPIVAEIRDELLRPGYERVLARRLGMDPTA"
     gene            complement(1287..2648)
                     /locus_tag="VN041_00640"
     CDS             complement(1287..2648)
                     /locus_tag="VN041_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWU27587.1"
                     /translation="MADPGYGAADAERFFAETHRSERNDFSRDRARVLHSAALRRLAA
                     KTQVLSPASTADFARNRLTHSLEVAQVGRELATALGVSEDVVDTACLSHDLGHPPFGH
                     NGERALNDWASDIGGFEGNAQSLRILTRLEAKVLDDQEHSVGLNLTRASLDATCKYPW
                     TIDNPLPDPGGRLKFGVYPDDEPVFHWMREGAPGRLRCIEAEIMDLSDDIGYSVHDFE
                     DAIVNGYLDVAQLSDPVEHHPLVGRIQQWVGYDYTREELADALYRLTRQPMWLKTFDR
                     SRQDLARLKNLTSDLIGRFARASVAATREAYPGPNLARYSGHVVVPRVVETEIAVLKG
                     IMGQAIVTIDARKGVYKEQRRVLTRLADALWSTDSLWSAGADALEPAFAADFLAATTD
                     GERARVVVDQVASLTDQTAVDWHNRLVGEIDPAEVGIWTPRHARPGAHRRPANRVVIE
                     GAV"
BASE COUNT          382 a          970 c          975 g          422 t
ORIGIN      
        1 ggccgtcggg tccatgccca gccgtcgtgc cagcacgcgc tcgtagccgg gacgcagcag
       61 ttcgtcgcgg atctccgcca cgatgggcgc ggcggcgcgc agggcgccca cctggccctc
      121 cacggtcgcg aggtcgtagc cggcgagctt gcggtcgatc gcgaactcga acatgggcac
      181 cttggcatcc atgagaccgc gcaccgcggc atccccgcgc tgcaggcgca ggtcgcacgg
      241 gtcgagaccg tccggcgcca cggccacgaa cgtctgcgcg gtgaagcggg agtcctccga
      301 gaaggcgcgc agcgcggcct tctgccccgc ctcgtcgccg tcgaacgtga agaccacctc
      361 accggccgcg gagtcatcgc ccatcacccg gcgcagcacc ttgatgtgct cggcggcgaa
      421 cgccgtgccg caggtggcga tggcggtggt cacgcccgcc agatggcacg ccatgacatc
      481 ggtgtagccc tccacgacca ccacgcgacg ggggtctccg cgggcgatgt cgcgcttggc
      541 gaggtcgagc ccgtagagca cctgcgtctt cttgtagagc ggcgtctccg gggtgttcag
      601 gtacttcggg cccttgtcgt cgtcgtagag tcggcgggcg ccgaagccga tggtctgccc
      661 ggtgacgtcg cggatgggcc agatcacccg gccacggaag cgatcgtaga cgccacgctg
      721 ccccgcggag acgagccccg cattggtgag ctcctcgcgg gtgaagccct gcgcggtgag
      781 cgccttggtc atgttctccc agcccttggg cgcgtagccg accccgaagt gcgcggctgc
      841 gcctgcgtcg aagccgcgct cccccaggaa ccgcctggcg ctctcggcct cagggctcag
      901 cagctggccg cggaagtact ccgcggcagc cgagttggcc gcgtacagcc tgctgcggcc
      961 gctggtctcg ggcgccgcac cgccgtcctc gtagtgcagc gggtaaccga tccggcccgc
     1021 gaggcgctcg accgcctcgg tgaagctgac gtgatccatc tcccgcagga acgagtacac
     1081 gtcgcccgag gcgccgcatc cgaagcagtg gtagtacccc acctgcggcc ggacgtggaa
     1141 gctggggctc ttctcgtcgt ggaacgggca cagccctttc atcgacccca cacccgccga
     1201 cttcagcgcg acgcgctcac cgacgatgtc cgcgatgttg gtgcgcgact tgacctcttc
     1261 gacatccgcc tgcaggatcc ggggcatcag acggcccctt cgatgaccac ccggttcgcg
     1321 gggcggcggt gcgctccggg gcgggcgtgg cggggcgtcc agatgccgac ctcggccgga
     1381 tcgatctcgc cgaccagacg gttgtgccag tcgacggcgg tctggtcggt gaggctggcc
     1441 acctggtcca cgaccacccg tgcacgctcg ccatccgtcg tcgccgcgag gaagtcggcg
     1501 gcgaaggcgg gctcgagggc atcggcaccg gccgaccaca gcgagtccgt cgaccacagc
     1561 gcgtctgcga ggcgcgtgag cacgcgacgc tgctccttgt agacgccctt gcgggcgtcg
     1621 atcgtgacga tcgcctgccc catgatgccc ttgagcaccg cgatctcggt ctcgacgacg
     1681 cggggcacga cgacgtgccc gctgtagcga gcgaggttcg gccccgggta cgcctcgcgg
     1741 gtcgccgcga cggatgcgcg ggcgaagcgg ccgatcaggt cgctggtgag gttcttcagc
     1801 cgggccagat cctgccgcga gcggtcgaag gtcttcagcc acatgggctg acgggtcagg
     1861 cgatacagcg cgtccgcgag ctcctcgcgg gtgtagtcgt agccgaccca ctgctggatc
     1921 cgcccgacga gcggatggtg ctcgaccggg tcggacagct gggcgacgtc gaggtagccg
     1981 ttcacgatcg cgtcctcgaa gtcgtgcacc gagtagccga tgtcgtcgct gaggtccatg
     2041 atctcggcct cgatgcagcg caggcgtccc ggcgccccct cgcgcatcca gtggaagacc
     2101 ggctcgtcgt ccggatacac cccgaacttc agccgcccac ccgggtcggg cagcgggttg
     2161 tcgatggtcc acgggtactt gcacgtcgcg tcgaggctcg cccgggtgag gttcagcccg
     2221 acggagtgct cctggtcgtc gagcaccttc gcctcgagtc gggtcaggat gcgcagcgac
     2281 tgcgcgttgc cctcgaaacc gccgatgtcg gaggcccagt cgttcagcgc ccgctcgccg
     2341 ttgtggccga acggcggatg ccccaggtcg tggctcaggc aggcggtgtc gaccacgtcc
     2401 tccgagacgc ccagggcggt cgcgagttcg cgacccacct gcgcgacctc gagcgaatgc
     2461 gtgaggcggt tgcgggcgaa gtccgctgtc gaggccgggc tcagcacctg cgtcttggcg
     2521 gcgagccgcc gcagtgcggc ggagtgcagc acgcgggcgc ggtcgcggga gaagtcgttg
     2581 cgctcggagc ggtgggtctc cgcgaagaag cgctcggcgt cggcggcgcc gtaccccggg
     2641 tcagccacca ctgttctcga tctccgctcc gcgcatcatg tcgctcacgt cggcggcgat
     2701 ctcgcgcgag tccagccagc cgtccgggag cgccgtgcgc ttcggggac
//
LOCUS       DATMSX010000020         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_95035,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000020 DATMSX010000000
VERSION     DATMSX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95035"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VN041_00645"
     CDS             <1..1169
                     /locus_tag="VN041_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWU27588.1"
                     /translation="ARASTAARPAPGRTGRIVGWLLVIVLLVGIALLSMRFIVTSPDL
                     GGTLNPQSPSPSGAKALAELVRDRGVDVEVTDSRAAAASALRPDTTLVLTDPYTLTDK
                     AVNTLLERADRVVLLTSSARMLRLLDLGEDAPLEAGSVDAQCDLPEFADVGDIRAERM
                     FTPSADADGCFRAESGAAAVLRSTEGGRTVTLVEGARLFSNDALAEDGNAALGLALLA
                     QTERVVWYQPSLSDSDISSDQKSLGSLTPEWVTPAILMLLVAGLAAIWWRGRRFGPLV
                     AESLPVTVRASETMQGRARLTAKAADAGHAGDSIRTGTVIRLSARLALGPRASIKEVA
                     DAASDRLGVPRTSLYDLLGGPAPQNDHELIDLARRLAELEAAVENTVHIERNRP"
     gene            1253..2176
                     /locus_tag="VN041_00650"
     CDS             1253..2176
                     /locus_tag="VN041_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU27589.1"
                     /translation="MHRVRAEVDKAVIGQAGTVTGLLVALLARGHVLLEGVPGVAKTL
                     VVRSFARAVGLDTKRVQFTPDLMPGDVTGSLVYDARTGEFEFREGPVFTNILLADEIN
                     RTPPKTQAALLEAMEERQVSADGISRTLPDPFLVAATQNPVEHEGTYTLPEAQLDRFL
                     MKLVVGMPERDAEVSVLRLHADGFSPRLLTGVQPAVSGDEIRAAQDAAARVRVTDDVL
                     GYVVDLARATRLSPSVELGASPRASTALLAAAKAWAWLNASTAVTPDHVQTMLMPVWR
                     HRLQLRPDAQMEGVSADAVLQSVVQQTRVPI"
     gene            2178..>2369
                     /locus_tag="VN041_00655"
     CDS             2178..>2369
                     /locus_tag="VN041_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU27590.1"
                     /translation="MFVTALLAPLVALGIVPIVLLSALGYPAWAVFGAWLLLCALLVG
                     LDVALAASPRAVVVTRQVPS"
BASE COUNT          315 a          863 c          842 g          349 t
ORIGIN      
        1 aagcgcgcgc cagcaccgcg gcacgtccag cgcccgggcg caccggccgc atcgtcggct
       61 ggctgctggt gatcgtgctg ctcgtgggca tcgcgctcct gtccatgcga ttcatcgtca
      121 cgagtcccga cctcggcggc acgctgaacc cgcagagccc cagcccgagc ggggcgaagg
      181 ctctggctga gctggtgcgc gatcgcggcg tggacgtcga ggtcaccgac agccgtgccg
      241 ccgcggcatc cgctctgcgc ccggacacca cgctggtgct gaccgatccg tacaccctca
      301 ccgacaaggc cgtgaacacc ctgctggaac gcgcggaccg cgtcgtgctg ctcaccagca
      361 gcgcccggat gcttcgcctg ctcgacctcg gagaggacgc ccctctcgag gcgggctcgg
      421 tcgacgcgca gtgcgacctg ccggagttcg cggatgtggg cgacatccgc gccgagcgga
      481 tgttcacccc ctccgcggat gccgacggct gcttccgtgc cgagtccggt gcggccgctg
      541 tcctgcgcag caccgaaggg ggccgcacgg tcaccctggt cgagggcgcg cggctgttct
      601 cgaacgacgc gctggccgag gacggcaacg ccgcgctcgg actcgccctg ctcgcgcaga
      661 cggaacgggt cgtctggtat cagccgtcgc tctccgacag cgacatctcc tccgaccaga
      721 agagcctcgg cagcctcacc cccgagtggg tcacgcccgc catcctgatg ctgctcgtcg
      781 caggtctcgc cgccatctgg tggcggggtc gccgcttcgg accgctcgtg gcggagtcgc
      841 tgccggtgac cgtgcgcgcc tccgagacca tgcagggtcg tgcgcgactc acggcgaagg
      901 ccgcggatgc agggcacgcc ggcgactcga tccgcaccgg cacggtcatc cgcctgtcgg
      961 cgcggctggc gctggggccg cgcgcctcga tcaaggaggt cgcggatgcc gcatccgatc
     1021 gcctcggtgt gccgcgcacc tcgctgtacg acctgctcgg cggtccggcg ccgcagaacg
     1081 accacgagct catcgacctc gcccgccgtc tcgcagaact cgaggcagcg gtcgagaaca
     1141 ccgtccacat cgaaaggaac cgcccgtgag cgacgagcag atcccccaca ccccggcccc
     1201 cgacgtccac gccggggcga tcccggcgga cgacgacgcc ctgcgccagg ccatgcaccg
     1261 ggtgcgcgcc gaggtcgaca aggccgtgat cggccaggcg ggcaccgtca cgggtctgct
     1321 ggtcgccctg ctcgcccgcg gccatgtgct gctggaaggc gtgcccggtg tcgccaagac
     1381 gctcgtggtg cgcagcttcg cccgcgcagt ggggctggac accaagcgcg tgcagttcac
     1441 gccggatctc atgcccggcg acgtgacggg gtcgctggtg tacgacgcgc gcaccggcga
     1501 gttcgagttc cgcgaggggc cggtgttcac gaacatcctg ctcgccgacg agatcaaccg
     1561 cacgccgccc aagacccagg ccgcgctgct ggaggccatg gaggagcgcc aggtgtccgc
     1621 cgacggcatc agccgcacgc tgccggaccc gttcctcgtc gcggcgacgc agaacccggt
     1681 cgagcacgag ggcacctaca cgctgcccga ggcgcagctg gaccggttcc tgatgaagct
     1741 cgtcgtcggc atgcccgagc gcgacgccga ggtctcggtg ctgcgtctgc acgccgacgg
     1801 cttctcgccg cgcctgctca ccggcgtgca gccggccgtg agcggcgacg agatccgcgc
     1861 ggcgcaggat gccgcggctc gcgtgcgggt gactgatgac gtcctcggct atgtggtgga
     1921 tctggcgcgc gcgacacggc tgtcgccctc ggtcgagctg ggtgcgagcc cgcgtgcatc
     1981 gaccgcgctg ctcgccgctg cgaaggcatg ggcatggctg aacgcctcca cggcggtcac
     2041 ccccgaccac gtgcagacca tgctgatgcc ggtctggcgc caccgcctgc agttgcgtcc
     2101 cgacgcgcag atggagggcg tgtcggcgga tgccgtgctg cagtccgtcg tgcagcagac
     2161 gcgggtgccg atctgacatg ttcgtgaccg cactgctggc gccgctggtc gcgctgggga
     2221 tcgtgccgat cgtgctgctg agcgcgctcg gctatccggc gtgggcggtg ttcggcgcgt
     2281 ggctgctgct gtgcgcgctt ctggtcgggc tggacgtcgc actcgcggcg agtccgcggg
     2341 cggtcgtggt cacgcgtcag gtgccgtcg
//
LOCUS       DATMSX010000021         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19242,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000021 DATMSX010000000
VERSION     DATMSX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19242"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VN041_00660"
     CDS             complement(<1..180)
                     /locus_tag="VN041_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWU27591.1"
                     /translation="MPAPLEALPLDGAVSAEALFLQLFARAEAAFWLDAGAGAADGWS
                     IIGTGDVESDQQAVRA"
     gene            224..880
                     /locus_tag="VN041_00665"
     CDS             224..880
                     /locus_tag="VN041_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWU27592.1"
                     /translation="MNDFVQWAIETVQSVDPVLRTVLAGVAIMLETSILIGLVVPGDT
                     VVLVAAIGVTSWAEGIALGLAVVVGALIGESLGFWLGRWAGPHIRSSWLGRRIGEQHW
                     ERSERYLRRRGGIAIFLSRFLPVLHSLVPLTVGMSGYAYRRFIAWTLPACVVWTTIYV
                     GLAAGAAGSYHELSQRIHYAGYVFVGAIAVLLIAVLVVKKLIARREERHMQDEPNREA
                     "
     gene            894..1919
                     /locus_tag="VN041_00670"
     CDS             894..1919
                     /locus_tag="VN041_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HWU27593.1"
                     /translation="MSSASAAKIHWFARLEHRFHVWRERRARRRGRSATIVPFPGYGG
                     TGWVRVTGRVLIVPPPPKRARGEGAGVRGWRAFVGIPVSFASVQVHLGGRTHDVVADR
                     GGVVDTVIEAHLEPGWQTFTMSVEGREPVEAAVFVVGDDARFGVVCDVDDTVMVTALP
                     RPFIAAWNSFVLDEHARTPVPGMAVLLEQLRRDHPGAPVIYLSTGAWNIAPTLRRFLS
                     RNLFPAGAMLLTDWGPTHDRWFRSGREHKARNLRRLAAEFPDVRWLLIGDDGQHDEII
                     YSEFLEEHPDSVAGVAIRRLLPAEAVLAGGRATVEHSPDTVPWVSAEDGAGLRDLLGE
                     AGIIDPR"
     gene            complement(1969..>3106)
                     /locus_tag="VN041_00675"
     CDS             complement(1969..>3106)
                     /locus_tag="VN041_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU27594.1"
                     /translation="SRHRRRAREVALRRTWTHDGIISVIVPAHNEAAGIEAAVRSIAA
                     SRRPVEIIVVDDGSTDGTADIVEALGLPQVTVIRQPNGGKPSALNTGIAAARGEIVVM
                     VDGDTVFEQDTVERLIQPFADPAVGAVSGNTKVVNRGGVLGAWQHIEYVVGFNLDRRL
                     FDIAECMPTVPGAIGAFRIETLRRVGGVSDDTLAEDTDLTMAICRDGWRVVYQEDARA
                     WTEAPASLGALWRQRYRWCYGTMQAMWKHRGAVLQKGPAGKLGRRGLGYLLVLQVILP
                     LFAPVVDIFAVYGLLFLDPMRIAVLWLAFLALQLGMAVYAFRMDGESLKPLWTLPLQQ
                     VVYRQMMYLVVIQSLFTALAGTRLRWHRMQRYGTLTVPPAVTTG"
BASE COUNT          441 a         1103 c         1093 g          469 t
ORIGIN      
        1 cgcccgcacg gcctgctgat cgctctcgac gtcacctgtg ccgatgatgc tccagccgtc
       61 cgcggcaccc gctccggcgt ccagccagaa cgccgcctcg gcgcgggcga acagctgcag
      121 gaacagcgct tcggcggaga cggccccgtc caggggcagg gcttcgagcg gggcgggcac
      181 gttcacagcg tagatccctg cgacagcatc tacgctcggt gaggtgaacg acttcgtaca
      241 gtgggcgatc gagacggtgc agtccgtcga cccggtgctg cgcacggtgc tcgcgggcgt
      301 cgcgatcatg ctggagacga gcatcctgat cgggctggtc gtccccggcg acaccgtcgt
      361 gctggtcgcg gcgatcgggg tcacctcgtg ggccgagggc atcgccctcg gactcgccgt
      421 cgtggtcggt gcgctcatcg gcgagtcgct gggcttctgg ctgggcaggt gggcgggacc
      481 gcacatccgc tcctcctggc tgggcagacg catcggcgag cagcactggg aacgatccga
      541 gcgctacctg cgccgccgcg gagggatcgc gatcttcctg tcgcgcttcc tgcccgtgct
      601 gcactcgctc gtacccctca cggtcgggat gagcggctac gcctaccggc ggttcatcgc
      661 ctggacactc cccgcgtgcg tcgtgtggac gacgatctac gtgggtctgg cggcgggcgc
      721 cgccggcagc tatcacgagc tctcgcagcg catccactac gcgggctatg tcttcgtcgg
      781 cgccatcgcg gtgctgctga tcgccgtgct ggtcgtcaag aagctcatcg cccggcgcga
      841 ggagcgccac atgcaggatg agccgaaccg ggaagcgtga aagactggac ccgatgtcct
      901 ctgcctcagc ggccaagatc cactggttcg ctcgcctcga acaccgcttc cacgtctggc
      961 gcgaacgccg cgcccgtcga cgtggccgtt cggcgacgat cgttccgttc cccggctacg
     1021 gcggaaccgg ctgggtgcgt gtcacgggtc gggtgctcat cgtgccgccg ccgccgaagc
     1081 gcgcccgcgg tgagggcgcc ggagtgcgcg gctggcgcgc gttcgtcggc atcccggtga
     1141 gcttcgcctc ggtgcaggtg cacctgggcg gccggaccca cgacgtggtc gccgatcgcg
     1201 gtggcgtggt ggacacggtc atcgaggcgc acctcgagcc cggctggcag acgttcacga
     1261 tgtcggtgga ggggcgcgag cccgtcgagg ccgccgtgtt cgtggtcggc gacgacgcac
     1321 gcttcggcgt ggtgtgcgat gtggacgaca ccgtgatggt gacggcgctc ccccggccgt
     1381 tcatcgcggc ctggaactcc ttcgtgctgg acgagcacgc gcgaaccccg gttccgggca
     1441 tggcggtgct gctcgagcag ctgcgccgcg atcaccccgg cgcgccggtg atctacctgt
     1501 cgaccggagc ctggaacatc gcgccgacgc tccgccggtt cctgagccgc aacctcttcc
     1561 ccgccggcgc catgctgctc accgactggg gccccaccca cgaccgctgg ttccgaagcg
     1621 gacgcgagca caaggcccgg aaccttcgcc gcctggcggc ggagttcccc gatgtgcgct
     1681 ggctgctgat cggcgatgac ggccagcacg acgagatcat ctactccgag ttcctcgagg
     1741 agcacccgga ctccgtcgcc ggtgtcgcga tccggcgcct gctgcccgcc gaggctgtcc
     1801 tggccggcgg tcgtgcgacg gtggagcaca gcccggacac ggtgccgtgg gtgagcgccg
     1861 aggacggcgc aggcctgcgc gatctgctcg gcgaggccgg catcatcgac ccgaggtgat
     1921 ccccggcacg gcaggtccgt ccggaatcag cacgcgggcg cgcgacggtc agcccgtcgt
     1981 caccgcaggc gggaccgtca gcgtgccgta gcgctgcatc cggtgccagc gcagccgggt
     2041 gcccgccagt gcggtgaaca gcgactggat caccaccaga tacatcatct gccggtagac
     2101 gacctgctgc agcggaagcg tccacagcgg cttgagcgac tccccgtcca tgcggaacgc
     2161 gtacaccgcc atgcccagct gcagggcgag gaaggccagc cagagcacag cgatccgcat
     2221 cgggtccagg aagagcagac cgtacaccgc gaagatgtcg acgaccggtg cgaacagcgg
     2281 caggatcacc tgcagcacga gcaggtaccc gagcccccgt cgtccgagct ttccggccgg
     2341 gcccttctgc agcacggcgc cgcggtgctt ccacatcgcc tgcatggtcc cgtagcacca
     2401 gcggtagcgc tgccgccaca gcgccccgag cgaggccgga gcctcggtcc aggcgcgcgc
     2461 gtcctcctgg tacaccacgc gccagccgtc acggcagatc gccattgtga ggtcggtgtc
     2521 ctccgcgagc gtgtcgtcac tcaccccgcc gacgcggcgc agcgtctcga tgcggaacgc
     2581 accgatcgcg cccggcacgg tgggcatgca ctcggcgatg tcgaagagcc ggcggtcgag
     2641 gttgaagccg accacgtact cgatgtgctg ccacgcgccc agcacgccgc cgcggttgac
     2701 gaccttcgtg ttccctgaga ccgcgccgac ggcgggatcc gcgaacggct ggatgaggcg
     2761 ctccacggtg tcctgctcga agaccgtgtc cccgtcgacc atcacgacga tctcgccgcg
     2821 ggcggcggcg atgccggtgt tgagcgccga cggctttccg ccgttgggct gacggatcac
     2881 ggtcacctgc ggcaggccca gcgcctcgac gatgtcggcc gtgccgtcgg tggagccgtc
     2941 gtcgacgacg atgatctcga ccggacgacg ggatgctgcg atcgaccgca cggcggcctc
     3001 gatgcccgcc gcctcgttgt gggcggggac gatgaccgag atgatgccgt cgtgcgtcca
     3061 ggtccggcgc agcgccacct cgcgggcccg gcgccggtgc cgggac
//
LOCUS       DATMSX010000022         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_133011,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000022 DATMSX010000000
VERSION     DATMSX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133011"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VN041_00680"
     CDS             complement(<1..903)
                     /locus_tag="VN041_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWU27595.1"
                     /translation="MLETLTVRDAVELAVVERSGFVESRHAGAAVVLSPEGDIVAQHG
                     NADALILPRSSLKPLQAVASLTAGAALEGEQLALGTASHSGTDRHAEVVREILAAGGL
                     TEDDLGCPPAWPSDAATRREMISDHAAESRIRMNCSGKHALMLRACVATGWPTAGYLD
                     PAHPLQVHIREVVERLTGEKIAHTSVDGCGAPVHAITLTGLARGIHRIGSASERSPFA
                     LHRVAGALVRAVRENPWTIAGPGEPDTIAIETLGVFAKGGAEGVMVMVAPNGATVALK
                     MLDGNGRAATIVAATLLARAGALTD"
     gene            1002..1505
                     /locus_tag="VN041_00685"
     CDS             1002..1505
                     /locus_tag="VN041_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWU27596.1"
                     /translation="MAQHPLGEHRYALTATWTGDRGSGTSGYRDYARDVTISIAGKPD
                     LLASADRPFRGDPGRWNPEDMLIAALAECHLLSYLHACVTAGVVVTSYRDEATGLMRE
                     DGRGGGAFAEVVLHPKVTVADASMIEAAEQAHHQAHDWCFIANSVNFPVRHEATVTVA
                     PRPLAGP"
     gene            complement(1580..2269)
                     /locus_tag="VN041_00690"
     CDS             complement(1580..2269)
                     /locus_tag="VN041_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU27597.1"
                     /translation="MVATPLARIVYRPRIEGRENVPKRGAVILASNHLSFIDSMAIPV
                     AAPRPVHFLAKSSYFEGTGMKGALSREFFTSVGAIPVRRGAGQAALDALDQQRQLLDQ
                     GLAVALYPEGTRSTDGRLYKGRTGVAFLALQTGAPVVPVGLIGTDHVMPVGAKMPSVK
                     ERITVRFGQPIDVSKHGPATSGKARRLATDEIMAAIHALSEQELAGVYNEPPAQNPIE
                     KIKQALPHERR"
     gene            2480..3436
                     /locus_tag="VN041_00695"
     CDS             2480..3436
                     /locus_tag="VN041_00695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWU27598.1"
                     /translation="MRLRPLALLSTIAVSALLLAGCAGSASPSPTSTSDATGGDVCAQ
                     TAEPGAISDGVKVDGQFGTPSKATFTLKQDIKKLQRTVITKGDGATVKKGEFVSYALS
                     AFNTTTGEHIGDAGYPGTEQMLPQNVLANPTLAQVIGCATVGSRLAVAFPTGSNGAES
                     QIYIVDVLGTVPTAAWGEEQAPVDGMPEVKLAKNGEPSISIPKTDPPTELKVSVLKQG
                     DGEAVADGDTTLLQYYGVNWADGKSFDSSWKNGEPISIPGNTYVPGFVQALAGQKVGS
                     QVLVVIPPALGYGDKEQGSIPANSTLVFVIDILATQHAPAAK"
     gene            3508..4590
                     /gene="dxr"
                     /locus_tag="VN041_00700"
     CDS             3508..4590
                     /gene="dxr"
                     /locus_tag="VN041_00700"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWU27599.1"
                     /translation="MRRIIVLGSTGSIGTQALDVIRANPRRFELVGISAGSNAEMMAR
                     QAEEFRLEHTALGAAESEQLVRDVEADVVLNAITGSIGLGPTLAALKSGRTLALANKE
                     SLIVGGALVRAAAERDQIVPVDSEHSAIAQALRSGTAAEVRRLVVTASGGPFRGRTRA
                     ELGDVTPAEALAHPTWDMGRMVTTNSATLVNKGLEVIEAHLLFDVPYDDIEVVVHPQS
                     IVHSMVEFVDGSTIAQASPPDMRLPISLGLDWPNRVGGVGKPLDWRQATSWTFEPLDG
                     EAFPSVGLAKSVGRAGGTFPAVYNAANEQAVDAFHEGRLPFLGIVDTIERVIEAHDAP
                     DDLTLETLAEAETWARAKADALIASA"
BASE COUNT          714 a         1657 c         1622 g          685 t
ORIGIN      
        1 atccgtcagc gcccctgctc gagcgagcag cgtcgcggcg acgatcgtcg cggcacggcc
       61 gttgccgtcg agcatcttca gcgccacggt cgcgccgttc ggggcgacca tgaccatgac
      121 gccctcggca ccgcccttcg cgaacacgcc gagcgtctcg atcgcgatcg tgtccggctc
      181 accgggaccg gcgatcgtcc acgggttctc gcgcacggcg cgcaccaggg cgccggccac
      241 gcgatgcagc gcgaacgggg agcgctccga cgcggagccg atccggtgga tgccgcgggc
      301 gagcccggtc agcgtaatcg catgcaccgg cgcaccacag ccgtcgaccg acgtgtgcgc
      361 gatcttctca ccggtgagcc gttcgacgac ctcgcggatg tgcacctgca gtggatgggc
      421 gggatccagg tatcccgcgg tcggccatcc cgttgccacg caggcgcgga gcatgagcgc
      481 gtgcttgccg gagcagttca tccggatgcg ggactccgcg gcgtgatcgc tgatcatctc
      541 ccgccgggtg gcggcatccg aaggccaggc gggagggcag cccagatcgt cctcggtcag
      601 accaccggcg gcgaggatct cccgcacgac ctcggcgtgt cgatcggtcc cggaatggct
      661 ggccgtgccg agcgcgagct gctcgccctc cagcgccgcg ccggcggtga ggctggccac
      721 cgcctgcagc ggcttgaggc tcgagcgggg gaggatcagg gcatccgcgt tcccgtgctg
      781 ggcgacgatg tcgccttccg gcgacagcac gaccgcggcg cccgcgtggc gggactccac
      841 gaagccgctg cgctccacga cggcgagttc gacagcatca cggacagtaa gggtctccag
      901 caccagacca gcctaatgcg ccgcatcgca cgatcctgct gacgacgtgg cccctgccgc
      961 gggaccgtcc cgtcgctccc ggaagatgaa agactggcgg catggcacag catccgctcg
     1021 gcgagcaccg gtacgcactc accgccacct ggaccggcga ccgcggcagc ggcacgagcg
     1081 gctaccggga ctacgcgcgc gacgtgacga tctcgatcgc cggcaaaccg gacctgctcg
     1141 cctccgccga ccgtccgttc cgcggcgatc ccggccgatg gaatcccgag gacatgctga
     1201 tcgctgcgct cgccgagtgc catctgctct cctacctgca cgcctgcgtc acggcggggg
     1261 tcgtcgtcac ctcgtaccgc gatgaggcga ccgggctcat gcgcgaggac ggccgcggcg
     1321 ggggagcgtt cgccgaggtg gtgctgcatc cgaaggtgac ggtcgccgat gcgtccatga
     1381 tcgaggctgc cgagcaggcc caccaccagg cgcacgactg gtgcttcatc gcgaactcgg
     1441 tgaacttccc ggtccgccat gaggcgacgg tgaccgtcgc cccgcgacct cttgccggtc
     1501 cctgagaccg ggcccacgcg accccgaggc tccgggcctc gcggagcgac agctgaagtg
     1561 gggcgtgggg agcgaagggt cagcgccgct cgtgcggcag cgcctgcttg atcttttcga
     1621 tcgggttctg cgccggcggc tcgttgtaca cgcccgcgag ctcctgctcg ctcagcgcgt
     1681 ggatggcggc catgatctcg tcggtcgcga gtcggcgcgc cttgccgctg gtggcgggac
     1741 cgtgcttgga cacgtcgatg ggctgaccga agcgcaccgt gatgcgctcc ttgaccgacg
     1801 gcatcttcgc acccacgggc atcacatgat ctgtgccgat caggccgacc ggaacgacgg
     1861 gcgcaccggt ctgcagggcg aggaaggcga cgccggtccg gcccttgtac agccggccgt
     1921 cggtggaccg cgtcccctcc gggtagaggg ccaccgcgag gccctgatcg agcagctgcc
     1981 gctgctgatc cagggcgtcg agcgcggcct gaccggcacc gcggcgcacc gggatcgcgc
     2041 cgacggaggt gaagaactcc ctcgagagcg cccccttcat acctgtgccc tcgaagtagc
     2101 tcgacttcgc gaggaagtgc acggggcggg gggcggcgac ggggatcgcc atcgagtcga
     2161 tgaacgagag gtggttgctg gcgaggatca ccgcgccgcg cttcggtacg ttctcgcgcc
     2221 cttcgatgcg gggacggtac acgatccggg cgaggggagt ggccaccatg cgacccaccg
     2281 cgtacttgaa tccggcatgt cggggggtgt gcgattccgg ttcgtgctcg gacccgaccg
     2341 actgctcaga actcatccgt gaagtgtatc gagcgccgca ttccggtcac ggaatgtcct
     2401 cgatgcgtgt tcgttcagcc gcggcaaagc cggatgcgcg aagatggaca cttcccatca
     2461 gcgattccga ggtctcatct tgcgcctgcg tcccctggcc ctcctttcca ccatcgccgt
     2521 ctcggcgctg cttctggccg gctgcgccgg cagcgcgtcg ccgagtccga cctcgacgtc
     2581 cgacgccacg ggcggtgacg tctgcgcgca gaccgctgag ccgggtgcga tctccgacgg
     2641 cgtcaaggtc gacggccagt tcggcacgcc gtcgaaggcc accttcacgc tcaagcagga
     2701 catcaagaag ctgcagcgca cggtgatcac caagggcgac ggggcgaccg tcaagaaggg
     2761 cgagttcgtc tcgtacgccc tcagcgcgtt caacacgacc accggcgagc acatcggtga
     2821 cgcaggctac ccgggcaccg agcagatgct gccgcagaac gttctcgcga accccaccct
     2881 cgcccaggtg atcggctgcg ccacggtcgg ctcgcgcctg gccgtcgctt tcccgaccgg
     2941 ctccaacggc gccgagtccc agatctacat cgtcgatgtg ctcggcaccg tcccgacggc
     3001 tgcgtggggc gaggagcagg cacccgtcga cgggatgccc gaggtcaagc tggccaagaa
     3061 cggcgagccc agcatctcga tcccgaagac cgacccgccc accgagctga aggtgtcggt
     3121 gctcaagcag ggcgacggcg aggcggtcgc cgacggcgac accactctgc tgcagtacta
     3181 cggcgtgaac tgggccgacg gcaagagctt cgactcgtcg tggaagaacg gcgagcccat
     3241 ctccatcccc ggcaacacgt acgtgcccgg tttcgtgcag gctctggccg gtcagaaggt
     3301 cggctcgcag gtgctggtcg tcatcccgcc ggcgctcggc tacggcgaca aggagcaggg
     3361 atcgatcccc gcgaactcga cgctcgtgtt cgtgatcgac atcctggcca cgcagcacgc
     3421 gcccgccgcc aagtaggcat tcctcgcacc gtcagtgcgc cggccccgct cctgcagggc
     3481 cggcgcactc tcgcataggc tggcggcatg cgtcgcatca tcgtcctcgg ctccaccggc
     3541 tccatcggca cgcaggccct ggatgtgatc cgcgccaatc cccgccgctt cgagctggtc
     3601 ggcatctcgg cggggtcgaa cgcggagatg atggcccgtc aggcggagga gttccgcctc
     3661 gagcacacgg cgctcggcgc ggcagagtcg gagcagctgg tccgcgacgt ggaggcggac
     3721 gtcgtgctca acgccatcac gggctccatc gggctcggac cgacgctggc cgcgctgaag
     3781 tccgggcgga ccctcgccct ggccaacaag gaatcactga tcgtcggcgg tgcgctcgtg
     3841 cgcgccgcag ctgagcggga ccagatcgtt ccggtcgact ccgagcactc ggcgatcgcg
     3901 caggcgctgc gatcgggcac cgccgcagag gtgcggcggc tggtcgtcac agcatccggc
     3961 gggcccttcc gaggccgcac cagggcggag ctcggcgacg tgacgcccgc ggaggccctg
     4021 gcgcacccga cctgggacat ggggcggatg gtcaccacca actccgccac cctcgtcaac
     4081 aagggcctgg aggtcatcga ggcgcacctt ctcttcgacg tgccgtacga cgacatcgag
     4141 gtggtcgtgc acccgcagtc gatcgtgcac tcgatggtcg agttcgtcga cggctccacg
     4201 atcgctcagg cgtcgccccc ggacatgcgt ctgccgatct cgctcgggct ggactggccg
     4261 aaccgcgtcg gcggggtggg caagccgctg gactggcggc aggcgaccag ctggacgttc
     4321 gagcccctcg acggcgaggc gttcccgtcc gtcggcctcg cgaagtccgt cggccgcgcg
     4381 ggtggcacgt tccccgcggt ctacaacgcc gccaatgagc aggccgtgga cgccttccac
     4441 gagggccggc tgccgttcct cggcatcgtc gacacgatcg agcgtgtcat cgaggcgcac
     4501 gacgccccgg acgacctcac gctcgagacg ctggcggagg ccgagacgtg ggcgcgcgcc
     4561 aaggcggacg cgctgatcgc ctccgcctga ggcgcgaaca cccgtcgcga ggactgtccc
     4621 cggtcagacc cggtcgtcgt aggggacggg ccagtgcgag tcgggggcgg gccagccg
//
LOCUS       DATMSX010000023         8818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_66659,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000023 DATMSX010000000
VERSION     DATMSX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66659"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VN041_00705"
     CDS             complement(<1..225)
                     /locus_tag="VN041_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix family transcriptional regulator"
                     /protein_id="HWU27600.1"
                     /translation="MSNTALLERPVTAPVRVISAADAAADLPSIRSPRGFALYVGIDE
                     IKAAASGVSLPVLVDALRRTLAELAPNAETH"
     gene            565..873
                     /locus_tag="VN041_00710"
     CDS             565..873
                     /locus_tag="VN041_00710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27601.1"
                     /translation="MTQPAHASGFDLKNEQDAARYTLTRDGALVSVLDYRDDGRTIAL
                     TRAFTIPTFRGNGYAAEIVAGAVAEIEARGDRKVNPVCWYVADWFEANPDKAHLLRGR
                     "
     gene            1030..2745
                     /locus_tag="VN041_00715"
     CDS             1030..2745
                     /locus_tag="VN041_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU27602.1"
                     /translation="MTLTKRSLTATAVLVAGILALGGCAATAEPTTGNAKVDLATMKD
                     EFYKATDPSKSPAAANARTDAFVTTISKPGGVFLPGFYDNGWDGNAVQPIFASLVVID
                     DAGKPVADLADKWEVSDDDLTYTFHLRKGLTFSDGSPLTADDVAFTLTLLNDPQYAGG
                     TDFSDIVIKGTDEYKNGSATSLSGIEVKDPQTIRITTEKPNPLALQTLGGQVISKAYY
                     GKDYQKGQLDYLKALYGKPLGAGPYALDEYVEGQEIRYKANEHYYGGKPEVPRVIFKV
                     VSSDSALQNFQNGDIDHGGFGSDPAVLKQLQGLGFANIRARVVPDIGAIWVNNKKPAL
                     ASTEVRQALYYGLDRQQIVDAKYKGLGQVADVFAAPTQWSFTTEGVTKYAYDPKKAEK
                     LLDDAGWKKGSDGVRQKDGQKLKLSYITTKDDDPIIPIAESTYTKIGIDFVPEVLDSN
                     TAFDRLYNGDYDLAGFRTNGLSDPNDAVSEFGTDDPDINVSGYQNAEVTKLIKKGIST
                     FDEKERQKIYTELYQKLSQDPPIILIDYRKSLSAWNARIEGGDKYVTGSGDAALALAK
                     LKIKN"
     gene            2848..3822
                     /locus_tag="VN041_00720"
     CDS             2848..3822
                     /locus_tag="VN041_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27603.1"
                     /translation="MTRYILKRLAVMVLLLVGVSVLVFFLFALMPGDYYSSNRTLTPE
                     RKAELRALAGLDQPVIVQYFIWLGNMLRGQFGYSLVYNRDVTEVLAPLIWNSFIVAFA
                     AFVLTWLIAIVSGVLASVRQYSWFDKLVTVGLFASLSVPSFFIGLLMIKIFALDLQWL
                     PTGGMLDTASSSTGAARVLEIMRHMVLPVAILTFLGAGSLTRYFRTGMLEVLRADFIR
                     TARAKGLKERSVVFSHALRNALLPAITLLAFELPGLFAGAIITEQIFNWPGVGRIQLE
                     SVQTRDYAVLMTITMLLAALTVLGSFLADILYAVADPRVRLVRAGSRA"
     gene            3819..4793
                     /locus_tag="VN041_00725"
     CDS             3819..4793
                     /locus_tag="VN041_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27604.1"
                     /translation="MSGGGELIVSQAVDPEIGPGQPHPDAGARSGRSGRRRSLWGESF
                     ARLIRMPAASISLATLVFMLLFSFVGPLLTGYTGDELNLPIANHIPTMDHPLGTDEYG
                     RDVLTRLMLAGRISLTIGIASMLLSLLLGAVLGIIAGYYGGIVDSIIMRVADLLMSIP
                     GLPLLIVMAAVLSELKVDPSSRIYIVMLLLSAIGWPSLARLIRGQVLSLREAPYMKAA
                     EVLGLSAASRQFRHLLPNVMPLLIVVATLSTASGILSESALSFLGLGVVPPNASWGNM
                     ISAANNLIDFQQHWWLWVPPGVALLITIAAINVFGDRLRDVFDPRMRK"
     gene            4790..5683
                     /locus_tag="VN041_00730"
     CDS             4790..5683
                     /locus_tag="VN041_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27605.1"
                     /translation="MSAVAAEDALTREDALVHVEELTVRFPVEGGHRTVVDDVSFEVH
                     AGEVLGIVGESGSGKSITSLSLLNLVDDPGEIVSGRVLLDGADVLKMRGTALQRVRGA
                     EIGMIFQEPMRSLNPVFTIGKQLIETIRAHEDVKPADAYDRSVDWLRRVGLPNPERVM
                     RSYPHEMSGGMLQRAMIAVALCCRPRLLIADEPTTALDVTIQAQIIDLLLDLRDETGM
                     AMLFITHDLGVIAEVADSVLVMYGGHVVERGDVFSLFDHPQHPYTDALLKARPMPGQR
                     LDRLPTISDEVRRIAAAGVTA"
     gene            5680..6618
                     /locus_tag="VN041_00735"
     CDS             5680..6618
                     /locus_tag="VN041_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27606.1"
                     /translation="MTGAAAVGEPVAQETVVLDATDVVKHFTSRGEGHGAAKQTVKAV
                     DGVTLRIREGEMFGLVGESGSGKTTFGQSVLRMHEPTSGSVRFRGEEILGLRTRELRR
                     LRPKMQYIFQDPFGALNPRLSVAAAIGEPMRAHGLASRNDERDRVAEVLEQCGMDADA
                     MDRYPHEFSGGQRQRIVIARAMALRPEFVVADEPVSALDVSVQGQIVNLLTDLRDQQR
                     TSFLFISHDLSVVEHMCTSVAIMYRGRIVEQGTRDQIFTDARHPYTRELLAAVPIADP
                     HQRGRRRGRVVTREWDDPDAVRPELVDVGDGHLVAL"
     gene            6640..7104
                     /locus_tag="VN041_00740"
     CDS             6640..7104
                     /locus_tag="VN041_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HWU27607.1"
                     /translation="MKLAEALAVRADLQRRIEQLRSRISDNARYQEGEEPAEDASALL
                     VEADAALDRLRELIRRINATNGRITVGDGTMTDALAARDVLRMRHSLLTDAAAAASGS
                     GQSFMRQMRSELRQFPALPVGELRSRADDVAKELRELDSSIQQANWGNDLDE"
     gene            7353..8507
                     /locus_tag="VN041_00745"
     CDS             7353..8507
                     /locus_tag="VN041_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HWU27608.1"
                     /translation="MRILHTSDWHIGRTFHGHSTLAALADVLDALVVQVRENDIDVVI
                     VAGDVFDSATPAASAYTLLDDALMALHETGATVIMTSGNHDSAARLGFHSRLLRDGIH
                     VLTDPQAIATPVTVHDEHGPVHFFGIPYLEPAIVRSLWPDAALRTQAQVMHHAMGLVR
                     DGMAEHTGRAVAVAHCFAAGVDPTAGLEREVRQGGLDVVPMASFAGPDYVALGHIHGR
                     QQLSERVRYSGAPLHYSFGEQHKERGSWLVELDSDGLASAQWLALPVPRALVTLTGTL
                     DEILSDENATAHADDWVCAIYTDALPQHEPMRRLRERLPFCALVQHQPEGTDAADDRS
                     YSQRLRTAVTDEERIEAFLEHVRAGQGASEREAELIREVLDDRVRAEALV"
     gene            8507..>8818
                     /locus_tag="VN041_00750"
     CDS             8507..>8818
                     /locus_tag="VN041_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU27609.1"
                     /translation="MQLHRLEVEGFGPFRARQVVEFDAFADDGIFLIAGRTGAGKSSI
                     LDAVCFGLYGGVPRYDGGEKRLRSDHSEPDDPTEVVVEFSTVAGRFRVTRSPAYERPA
                     RR"
BASE COUNT         1435 a         2993 c         3010 g         1380 t
ORIGIN      
        1 gtgggtctcg gcgttcgggg cgagctcggc cagcgtgcgg cgcagcgcgt cgacgagcac
       61 gggcaggctc acaccggagg cggccgcctt gatctcgtcg atgccgacgt agagggcgaa
      121 gccgcgaggc gagcggatcg aggggaggtc tgcggcggcg tcggcggcgg agatgacgcg
      181 gacgggggcg gtgacgggac gctcgaggag ggcggtgttc gacatgatga tggtccttgg
      241 aggatgcggg agctgtcggg ctccgggtgc gttgcacgaa atgaccggta tgagccggtg
      301 ggaatgagcg cgtcgggtgg gcgctcagag acctgcctct cagatggaga gacgaaaaca
      361 ggtcaatctg tcttgttgcg ccttagcaac acattcggca acacatgcca acgcgaccgg
      421 gcatcatcat cccggcagtc ctgttcgcct cctgggcgga caaagggctt gcgttggtgg
      481 tcatggggcc gattatttct catcgtgtcc ctctgtgtca aaacgagtca ttccggtcgt
      541 cgtcacacgg cgtaacgttg tggcgtgact cagcccgcgc atgcctccgg attcgacctg
      601 aagaacgagc aggatgccgc tcgctacacg ctcaccagag acggcgccct cgtgagcgtg
      661 ctcgactacc gggacgacgg tcgcaccatc gcactcaccc gtgcgttcac gatcccgaca
      721 ttccgcggga acggctatgc ggccgagatc gtggcgggcg ccgtggccga gatcgaagcc
      781 cgtggcgacc ggaaggtgaa ccccgtctgc tggtacgtcg ccgactggtt cgaggccaat
      841 ccggacaagg cgcatctgct gcgcggacgc tgatcgccgg catccgcccg ccacccgatt
      901 tctggactcg gactcgtaac accccgtcac gggacggccc cggcgggtct ccgccggggt
      961 tagtctcgct gagcacgcaa tcgtgacgct gggggacgca ttcacaagat gcttgagaag
     1021 gaagagcaca tgacgctgac gaagagatcg ctcaccgcca cagcggtgct ggtggcgggg
     1081 atcctcgcac tgggcggctg cgccgccacg gccgagccga ccaccggaaa cgcgaaggtc
     1141 gacctggcca ccatgaagga cgagttctac aaggcgaccg acccgagcaa gagcccggcg
     1201 gcggcgaacg cccgcaccga cgccttcgtc accaccatca gcaagcccgg gggcgtgttc
     1261 ctccccggct tctacgacaa cggctgggac ggcaacgccg tgcagcccat cttcgcctcg
     1321 ctcgtcgtca tcgatgacgc gggcaagcct gtcgccgacc tggccgacaa gtgggaggtc
     1381 tccgacgacg acctcaccta cacgttccac ctgcgcaagg gcctgacctt cagcgacggc
     1441 tcgccgctca ccgcagacga cgtcgcgttc accctcacgc tgttgaacga cccgcagtac
     1501 gccggtggca ccgacttcag cgacatcgtg atcaagggca ccgacgagta caagaacggc
     1561 tccgccacgt cgctgtcggg catcgaggtg aaggacccgc agaccatccg gatcaccacc
     1621 gagaagccca acccgctggc cctgcagacc ctcggcggtc aggtcatctc caaggcgtac
     1681 tacggcaagg actaccagaa ggggcagctc gactacctca aggcgctgta cggcaagccc
     1741 ctcggcgcag gcccgtacgc gctcgacgag tacgtcgagg gccaggagat ccgctacaag
     1801 gccaatgagc actactacgg cggcaagccc gaggttccgc gcgtcatctt caaggtcgtc
     1861 tccagcgaca gcgcgctgca gaacttccag aacggcgaca tcgaccacgg cggcttcggc
     1921 agtgaccccg ccgtgctgaa gcagctgcaa gggctcggct tcgcgaacat ccgcgctcgc
     1981 gtcgtgcccg acatcggcgc gatctgggtg aacaacaaga agcccgcgct ggcttcgacc
     2041 gaggtgcgcc aggcgctgta ctacggcctg gaccgtcagc agatcgtgga cgccaagtac
     2101 aagggcctcg gccaggtggc cgacgtgttc gccgctccca cgcagtggtc gttcaccacc
     2161 gaaggtgtca cgaagtacgc gtacgacccg aagaaggccg agaagctgct cgacgacgcc
     2221 ggctggaaga aaggctcgga cggcgtccgt cagaaggacg gccagaagct caagctgagc
     2281 tacatcacca ccaaggacga cgacccgatc atcccgatcg cggagagcac gtacaccaag
     2341 atcggcatcg acttcgtgcc cgaggtgctc gactcgaaca ccgcgttcga ccggctgtac
     2401 aacggcgact acgacctggc cggcttccgc accaacggcc tgtccgaccc caacgacgcc
     2461 gtcagcgagt tcggcaccga cgaccccgac atcaacgtgt ccggatacca gaacgccgag
     2521 gtcaccaagc tcatcaagaa gggcatctcc accttcgacg agaaggagcg gcagaagatc
     2581 tacaccgagc tctaccagaa gctcagccag gatccgccga tcatcctgat cgactaccgc
     2641 aagtcgctct ccgcctggaa cgcgcgcatc gagggcggcg acaagtacgt caccggcagc
     2701 ggcgacgcgg cgctcgccct ggcgaagctg aagatcaaga actgaggcgc acgcctctag
     2761 gatgcttccg ggacgggccg tggacccgcc ccggaagcat ccgccgtcgt atgcctgccg
     2821 gaacaatgat ctgagcgccc gcctgttatg actcgttaca tcctcaagag actcgccgtc
     2881 atggtgctgc tgctcgtcgg cgtctccgtg ctcgtcttct tcctcttcgc cctcatgccg
     2941 ggcgactact acagctccaa ccgcacgctc acaccggagc gcaaggccga gctgcgcgcg
     3001 ctcgccgggc tcgaccagcc cgtgatcgtg cagtacttca tctggctggg caacatgctg
     3061 cgcgggcagt tcggctactc gctggtgtac aaccgcgatg tcaccgaggt gctggccccg
     3121 ctgatctgga actcgttcat cgtggccttc gcggcgttcg tgctcacctg gctgatcgcc
     3181 atcgtctcgg gcgtgctcgc ctccgtgcgg cagtactcgt ggttcgacaa gctcgtcacg
     3241 gtcggcctgt tcgcgtcgct gtcggtgccc tcgttcttca tcggcctgct gatgatcaag
     3301 atcttcgcgc tcgatctgca gtggctcccc acgggaggga tgctcgacac cgcgagcagt
     3361 tcgacgggcg ccgcccgcgt gctggagatc atgcggcaca tggtgctgcc ggtagcgatc
     3421 ctcaccttcc tgggcgcggg gtcgctgacc cgctacttcc gcaccgggat gctggaggtg
     3481 ctgcgcgccg acttcatccg cacagccagg gcgaagggcc tcaaggagcg ctcggtggtg
     3541 ttctcgcacg ctctgcgcaa tgccctgctg ccggcgatca cactgctggc cttcgagctg
     3601 ccgggcctgt tcgccggcgc catcatcact gagcagatct tcaactggcc gggtgtcggc
     3661 cgcatccagc tggagtccgt gcagacccgc gactacgcgg tgctgatgac catcaccatg
     3721 ctgctcgcgg cgctcaccgt gctcgggagc ttcctggccg acatcctgta cgccgtcgcc
     3781 gacccgaggg tgcggctcgt ccgagcgggg agcagagcat gagcggcggc ggagagctca
     3841 tcgtcagcca ggccgtcgac cccgagatcg ggccgggaca gccgcatccg gatgccgggg
     3901 cacgatcggg ccgcagcggc aggcgccgct ccctgtgggg tgagtcgttc gcacgtctga
     3961 tccgcatgcc cgcggcgagc atcagcctcg cgaccctcgt cttcatgctg ctgttcagct
     4021 tcgtcgggcc gcttctcacc gggtacaccg gcgatgagct gaaccttccg atcgcgaacc
     4081 acatcccgac catggaccat ccgctgggca ccgacgagta cgggcgcgac gtgctgaccc
     4141 ggctcatgct cgcggggcgc atctcgctga ccatcggcat cgcgtccatg ctgctctcgc
     4201 tgctgctggg ggccgtgctc ggcatcatcg cgggctacta cggcggcatc gtcgacagca
     4261 tcatcatgcg cgtcgccgac ctgctgatgt ccatccccgg cctgccgctg ctgatcgtga
     4321 tggccgcggt gctgtccgag ctgaaggtcg acccgtcctc gcgcatctac atcgtcatgc
     4381 tgctgctcag cgccatcggc tggccgtcgc tggcccgcct gatccgcggg caggtgctgt
     4441 cgctgcgcga ggcgccgtac atgaaggcgg cggaggtgct gggactcagc gcggcatccc
     4501 gccagttccg ccacctgctg ccgaacgtca tgccgctgct gatcgtggtg gcgacgctga
     4561 gcacagcatc cggcatcctg tcggagtcgg cgctgagctt cctcggcctc ggcgtggtgc
     4621 ccccgaacgc ctcgtgggga aacatgatca gcgccgccaa caacctgatc gacttccagc
     4681 agcactggtg gctgtgggtg ccgcccggtg tcgcgctgct gatcacgatc gccgcgatca
     4741 acgtgttcgg cgaccggttg agggacgtgt tcgacccgag gatgaggaag tgagcgccgt
     4801 ggccgcggaa gatgctctga cccgcgaaga cgcactggtg cacgtggagg agctgaccgt
     4861 gcgcttcccc gtcgagggcg ggcatcgcac ggtcgtcgac gacgtgtctt tcgaggtgca
     4921 tgccggggag gtgctcggca tcgtcggcga gtccggttcg ggcaagagca tcacctcgct
     4981 gtcgctgctg aacctcgtcg acgacccggg cgagatcgtc tcggggcggg tgctgctgga
     5041 cggcgccgat gtgctgaaga tgcgcggcac cgcgctgcag cgggtgcggg gtgcggagat
     5101 cgggatgatc ttccaggagc cgatgcgctc gctcaacccc gtgttcacga tcggcaagca
     5161 gttgatcgag acgatccgcg cgcatgagga cgtgaagccg gccgacgcct acgaccgctc
     5221 ggtggactgg ctgcggcgcg tcggcctgcc gaaccccgag cgggtgatgc gctcctatcc
     5281 ccacgagatg tcgggtggga tgctgcagcg cgccatgatc gcggtggcgc tgtgctgccg
     5341 cccgcgcctg ctgatcgccg acgagcccac cacggcgctc gatgtcacga tccaggcgca
     5401 gatcatcgac ctgctgctgg acctgcgcga cgagaccggg atggccatgc tgttcatcac
     5461 ccacgacctc ggtgtcatcg ccgaggtcgc cgactccgtg ctggtgatgt acggcgggca
     5521 cgtggtcgag cgcggcgatg tgttctctct gttcgaccat ccgcagcatc cgtacaccga
     5581 cgcgctgctg aaggcgcgac cgatgccggg ccagcgactc gacaggctgc ccaccatctc
     5641 cgatgaggtg cgccggatcg cggcggcggg ggtgacggca tgaccggggc ggccgcagtc
     5701 ggggagcccg tggcgcagga gaccgtggtg ctcgatgcca cggacgtcgt gaagcacttc
     5761 acctcgcgcg gcgagggcca cggcgccgcg aagcagacgg tgaaggccgt cgacggcgtg
     5821 accctgcgga tccgcgaggg cgagatgttc gggctggtcg gggagtccgg cagcggcaag
     5881 accaccttcg gtcagtccgt gctgcggatg cacgagccga ccagcggctc cgtccgattc
     5941 cgcggcgagg agatcctcgg cctgcgcacc cgcgagctgc ggcgcctgcg tccgaagatg
     6001 cagtacatct tccaggatcc tttcggcgcg ctgaacccgc gcctctcggt ggcggcggcg
     6061 atcggcgagc cgatgcgcgc gcacggcctg gcgtcccgga acgacgagcg cgatcgggtc
     6121 gcggaggtgc tggagcagtg cggcatggac gcggatgcca tggaccgcta cccgcacgag
     6181 ttctccggcg ggcagcgtca gcgcatcgtg atcgcccgcg ccatggcgct gcgacccgag
     6241 ttcgtggtcg cggatgagcc ggtctccgcg ctggacgtgt cggtgcaggg gcagatcgtg
     6301 aacctgctga ccgacctgcg cgaccagcag cgcacctcgt tcctgttcat ctcgcacgac
     6361 ctcagcgtgg tggagcacat gtgcacgagc gtggcgatca tgtaccgcgg ccgcatcgtc
     6421 gagcagggca cgagagatca gatcttcacg gacgcccgtc atccgtacac gcgggagctg
     6481 ctggcggccg tgccgatcgc cgacccgcac cagcgcggtc ggcggcgggg gcgcgtcgtg
     6541 acgcgggagt gggacgaccc ggatgccgtg cgcccagagc tggtcgacgt cggcgacggg
     6601 cacctggtcg cactctgagc gatgtgtgag gatggcgagg tgaaactcgc cgaagcactc
     6661 gccgtccgcg ccgatctgca gcgccgcatc gaacagctcc gctcccgcat ctcggacaat
     6721 gcccgctacc aggagggcga ggagcccgcg gaggacgcgt ccgcgctgct cgtcgaagcc
     6781 gatgccgcgc tggaccggct gcgcgagctg atccgccgga tcaacgcgac caacggccgg
     6841 atcacggtgg gcgacggcac gatgacggat gctctggccg cgcgcgacgt gctgcggatg
     6901 cggcactcgc tgctcacaga cgcggcggca gcggcctccg gctccggcca gagcttcatg
     6961 cggcagatgc gctccgagct gcgtcagttc ccggctctcc cggtcggcga gctgcggagc
     7021 cgggcggatg acgtggccaa ggagctgcga gaactggaca gcagcatcca gcaggcgaac
     7081 tgggggaacg acctggacga ataacacgga agtcggtaat cgcgacgaag cgggcacacc
     7141 cccttggtcg gcggggcaag tcgacctgtc ccgggcggag ggcagcccat cacctgcatg
     7201 acgcagcccc gcattccgca ccggtgacag cgcagcgcgc aaggcgcatc accccgtgcc
     7261 ggtcgtcgcg gtgagggcgg gtcaggggat cttccgttgc cgtcccggca cgcctcagcg
     7321 gcgatgtcgg tggccgtccg tacgttggag gcatgcgaat cctgcacacc tccgactggc
     7381 acatcggtcg cacgttccac gggcattcca cgctcgcggc gctggccgac gtgctcgacg
     7441 cgctggtcgt gcaggtgcgt gagaacgaca tcgacgtggt gatcgtcgcc ggcgacgtgt
     7501 tcgactcggc cacacccgct gcatccgcct acacgctgct cgacgacgcc ctgatggcgc
     7561 tgcacgagac gggtgcgacc gtgatcatga ccagcggcaa ccacgactcc gccgcgcgtc
     7621 tcggtttcca ctcccgcctg ctgcgcgacg gcatccacgt gctcaccgac ccgcaggcga
     7681 tcgcgacgcc cgtcaccgtg cacgacgagc acggcccggt gcacttcttc ggcatcccgt
     7741 acctggagcc ggcgatcgtg cgcagtctgt ggccggatgc ggcgctgcgc acgcaggcgc
     7801 aggtgatgca ccacgcgatg gggctggtcc gtgacggcat ggcggagcac acgggccgtg
     7861 cggtcgccgt cgcgcactgc ttcgcggccg gcgtcgaccc caccgccgga ctcgagcggg
     7921 aagtgcgcca gggcggtctc gacgtcgtgc cgatggcgtc gttcgcgggc cccgactacg
     7981 tggcgctcgg tcacatccac gggcgtcagc agctcagcga gcgcgtgcgc tactcgggag
     8041 ccccgctgca ctacagcttc ggcgagcagc acaaggagcg cggatcctgg ctggtcgagc
     8101 tggactccga cgggctggcg agcgcgcagt ggctggcgct gcccgtgccg cgcgcgctcg
     8161 tcaccctcac cggcacgctc gacgagatcc tctccgacga gaacgccacg gcgcacgccg
     8221 atgactgggt gtgcgcgatc tacaccgacg cgcttccgca gcacgagccg atgcgccggc
     8281 tgcgcgagcg gctcccgttc tgcgcgctgg tgcagcacca gcccgagggg acggacgccg
     8341 ccgacgaccg ctcctactcg cagcgcctgc gcacagcggt gacggacgag gagcggatcg
     8401 aggcgttcct cgagcacgtg cgggccgggc agggcgcgtc cgagcgcgag gcggagctga
     8461 tccgtgaggt gctcgacgat cgggtgcgcg ccgaggcgct cgtctgatgc agctgcaccg
     8521 tctggaggtg gagggcttcg ggccgttccg tgcgcgccag gtggtcgagt tcgacgcctt
     8581 cgcagacgac gggatcttcc tgatcgcagg gcgcacgggc gccggcaagt cgtcgatcct
     8641 cgatgctgtg tgcttcggcc tgtacggcgg cgtcccgcgg tacgacggcg gggagaagag
     8701 gctgcgcagc gaccactccg agcccgacga ccccaccgag gtggtggtgg agttcagcac
     8761 ggtcgccggg cgcttccgcg tgacccggtc gcccgcctac gagcggccgg ccaggcgc
//
LOCUS       DATMSX010000024         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_28738,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000024 DATMSX010000000
VERSION     DATMSX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28738"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VN041_00755"
     CDS             <1..304
                     /locus_tag="VN041_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWU27610.1"
                     /translation="VPAAGPAAGADGLATASLIVSRPLDAWIALPGSAKGMVWLNGFL
                     LGRYWERGPQVTLYAPGPLWREGENEIVVLDTDRLGAAVEIRESADYGPREEFVGS"
     gene            449..4072
                     /locus_tag="VN041_00760"
     CDS             449..4072
                     /locus_tag="VN041_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWU27611.1"
                     /translation="MRVPLTHQHGRSPVRRTLRGALAALSAAVVVGALVTPLTASAAE
                     TDDLDIAFGGSLVGTTAYTTVEGETMRGVVRRMNGAEEQIPGGGVTLGGGTQGIRFDA
                     SAFTLADPGATSGFAAELSVTPAASQSDMATLFAAGGNLFVRIQQGELVYGFDSQAGG
                     SWKQNRRTAPVPAGGVEHDLSVQYIRVGTGAEMHVSIDGTALATVTADAPAGIDASAA
                     SSFGFGNDVHPAAGGRGYVGDLHRIRVASGLTAYDPAQNTYQPAPVTKTLMNTGFSGA
                     VDGTAYVPAAGEIVDGALATRGGATVSSGRLVISGAAQAADFTASADPFAGTALDAGF
                     VTELEFTTDVSQKDLATLVAVGGNFSARYRAGGLQYGFSSKASGSWKDHMSTIALPAN
                     GKAHVLSLAYQPLDGGGAEVIAWLDGEELPKVSATERSTRNTGVTSTASFGNEVPAIG
                     GRAFIGSFDRARFALLTETFSAQAFEYQSLKPVVPCADMTGVEPGNYVPISADDCPAN
                     VIAKSTLVRPTPQQLRWQELGLTAFLHFGINTFYDQEWGHGTEDPARFDPTGEVDVDG
                     WMKTLRDSGHRMAILTLKHHDGFVLYPSRYTDYDIAATPWKDGKGDLVREFTDAARKY
                     GMKVGLYMSPADSHEELTGRFGNGSPKSPRTIPTLVQDDDRAGRDLPGFTYDATDYGA
                     YFLNSLYEILTQYGQIDEVWFDGSNGNTEKQEIFDYPAFYDLIGKLQPNAVVAVGGRD
                     VRWVGNESGTARQNEWAPMAVDVPQDGGKIGVVAGGSFEQVGSADTLVSGARAGADAL
                     HWWPTESDMKLTQGWFAHPTDTPKSGPTLLNHYLNTVGRNSVMLLNNPPTTNGSFAPA
                     SVDALASFAAERRKAFGTDLALGVPVETPVGPSAALTDGDRRSAWKGDGAPATFTIDL
                     GTAQTITRVTLSEGVDVAGQSVDGFRIEAQVDGAWKRVGSAGTIGVSRIVTLDAPTTA
                     QRFRLVVDSARGPFALAKLALHPTLDADPGRLTEVWLDCTAERAGDGSKERPFSSLEQ
                     FRETELAPGAAVHLRAGTACADSDTPFWGYGIADAPIVVDVYDGHEPPTFGDRTAREV
                     FQGLEIQGWKIAFRDGEPTLSLGSGTVKVGDEVVVSVADFPASSEVSVGVEGAGRPRG
                     AITTDSQGAGSVVLRIAGLAPGAYQLIATGPEGTRASGTVVVEAGRSAAHRQDGGIEI
                     APA"
     gene            complement(4202..>4631)
                     /locus_tag="VN041_00765"
     CDS             complement(4202..>4631)
                     /locus_tag="VN041_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27612.1"
                     /translation="ADGEAVASAGGVAHGIRPWQDRRTHRTTEGTTMSDQVFIFIAAA
                     NARTSVLWIEILSAAGADPVIAEALEALLARRMADYRTTVQLLIDRGIAPADTDIPGA
                     AAALSFLWSPEGHQQLVLQSGWSMPRYTAWLCDAARRQLA"
BASE COUNT          696 a         1657 c         1603 g          675 t
ORIGIN      
        1 ggttcccgcg gcggggcccg ctgccggagc ggacggcctg gccacggcat ccctcatcgt
       61 ctcgcgcccg ctcgacgcgt ggatcgcgct gcccggctcc gcgaagggca tggtgtggct
      121 gaacggcttc ctgctcggac ggtactggga gcgcggcccg caggtcaccc tgtacgcccc
      181 cgggccgctg tggcgggagg gtgagaacga gatcgtcgtg ctcgacaccg accgactcgg
      241 cgccgccgtc gagatccgcg agagcgccga ctacggcccg cgcgaagagt tcgtcgggtc
      301 gtgatccggt cccgctgacc ggatacatcg ggtcagtgtt cagttcggtg cacgtcgtgt
      361 gccgaaatgc gagattccat tgttgataca tccgatctct gctacgttcg cggtgatccc
      421 catcccctaa cgcaatggag cagaaggaat gcgtgtcccc ctcacacacc agcacgggcg
      481 atcaccggtc cgccgcactc tgcgcggcgc gctcgccgcg ctcagcgccg ccgtcgtggt
      541 cggtgcgctc gtgacccctc tcaccgcctc ggcagctgag accgacgatc tcgacatcgc
      601 cttcggcggc tccctggtcg gtacgaccgc gtacacgacc gtcgagggcg agacgatgcg
      661 cggagtcgtc cgtcgcatga acggtgccga ggagcagatc cccggcggcg gggtgaccct
      721 cggcggtggc acgcagggca tccgcttcga cgcctccgcc ttcacgctcg ccgaccccgg
      781 cgcgaccagc ggcttcgccg ccgagctctc cgtcacaccg gcggcgtcgc agagcgacat
      841 ggccacactc ttcgcagccg gcggcaacct gttcgtccgc atccagcagg gcgagctcgt
      901 gtacggcttc gactcgcagg ccggcggaag ctggaagcag aaccgccgca ccgcgccggt
      961 gccggccggc ggcgtcgagc acgacctctc ggtgcagtac atccgggtgg ggaccggggc
     1021 cgagatgcac gtctcgatcg acggcaccgc cctcgccacg gtcaccgccg atgcccccgc
     1081 gggcatcgac gcatcggccg cgtcgtcctt cgggttcggc aacgacgtgc accccgcagc
     1141 gggaggccgg ggctacgtcg gcgacctgca ccgcatccgt gtcgcgtcgg gtctcaccgc
     1201 ctacgatccc gcgcagaaca cctaccagcc cgcacccgtc acgaagaccc tgatgaacac
     1261 cggattctcg ggtgccgtcg acggcaccgc atacgtgccc gccgcgggtg agatcgtcga
     1321 cggcgcgctc gcgacgcgcg gcggggcgac cgtgtcgtcc gggcgcctcg tgatctccgg
     1381 tgccgcacag gcggctgact tcacggcatc cgccgacccg ttcgcgggaa ccgccctgga
     1441 tgccgggttc gtgaccgagc tcgagttcac gaccgacgtc tcccagaagg acctcgccac
     1501 cctcgtggcc gtcggcggga acttctcggc acgctaccgt gcgggtggac tgcagtacgg
     1561 cttcagctcc aaggcgtcgg gttcgtggaa ggaccacatg agcaccatcg cccttccggc
     1621 gaacgggaag gcgcacgtgc tctcgctggc gtatcagcct ctcgacggcg gcggcgccga
     1681 ggtgatcgcg tggctcgacg gcgaggagct cccgaaggtg agcgccaccg agcgctccac
     1741 acgcaacacg ggcgtcacca gcacggcatc cttcgggaac gaggtgccag cgatcggcgg
     1801 ccgtgcgttc atcggttcct tcgaccgggc caggttcgcc ctgctcactg agaccttcag
     1861 cgcgcaggcg ttcgagtatc agtccctcaa gccggtcgtg ccgtgcgctg acatgacggg
     1921 cgtcgagccg ggcaactacg tgccgatctc ggcggacgac tgccccgcca acgtgatcgc
     1981 caagtccaca ctggtgcggc cgaccccgca gcagctgcgc tggcaggagc tcgggctgac
     2041 ggcgttcctg cacttcggca tcaacacgtt ctacgaccag gagtggggcc acggcaccga
     2101 ggacccggca cgcttcgacc cgaccggaga ggtcgacgtc gacggctgga tgaagacgct
     2161 gcgcgactcc ggtcaccgca tggcgatcct cacgctcaag catcacgacg gcttcgtgct
     2221 gtacccctcg cgctacaccg actacgacat cgcggcgacg ccgtggaagg acggcaaggg
     2281 cgacctggtg cgcgagttca ccgacgccgc gcgcaagtac ggcatgaagg tcgggctcta
     2341 catgtcgccc gccgactcgc acgaggagct caccggccgc ttcggcaacg gcagcccgaa
     2401 gtcgccgcgc acgatcccca ccctcgtgca ggacgatgac cgcgccggac gggacctgcc
     2461 cggcttcacg tacgacgcga ccgactacgg ggcgtacttc ctcaactcgc tgtacgagat
     2521 cctcacccag tacgggcaga tcgacgaggt gtggttcgac ggctccaacg gcaacaccga
     2581 gaagcaggag atcttcgact accccgcctt ctacgatctg atcggcaagc tgcagccgaa
     2641 cgccgtcgtc gcggtcggcg gccgggacgt gcgctgggtc ggcaacgagt cgggcaccgc
     2701 ccgccagaac gagtgggcgc cgatggcggt ggacgtgccg caggacggcg gcaagatcgg
     2761 cgtcgtcgcc ggaggctcgt tcgagcaggt cggttcggcg gacaccctcg tctccggtgc
     2821 cagggccggc gcggatgccc tgcactggtg gcccaccgag tcggacatga agctcacgca
     2881 gggctggttc gcgcacccga ccgacacgcc caagagcggg ccgacgctgc tgaaccacta
     2941 cctgaacacg gtgggccgca actcggtgat gctgctgaac aacccgccga ccacgaacgg
     3001 gtcgttcgcg ccggcctccg tcgatgccct cgcctcgttc gcggccgagc gtcgcaaggc
     3061 cttcgggacc gacctcgccc tcggcgtgcc ggtggagaca cccgtcgggc cgtccgccgc
     3121 gctcactgac ggcgaccgcc gctcggcctg gaagggcgac ggcgcgcccg cgaccttcac
     3181 gatcgatctc ggcaccgccc agacgatcac ccgcgtgacc ctgtcggagg gcgttgacgt
     3241 cgccggccag tccgtcgacg gattccgcat cgaggcgcag gtcgacggtg cctggaagag
     3301 ggtcggatcc gcgggcacca tcggtgtcag ccggatcgtg accctggatg cgccgaccac
     3361 cgcccagcgg ttccgcctgg tcgtcgactc cgcgcgaggg ccgttcgccc tggcgaagct
     3421 ggccctgcac ccgacgctcg acgccgaccc aggccgcctg accgaggtgt ggctggactg
     3481 cacggccgag cgcgcgggtg acggttcgaa ggagcgcccg ttctcgtcgc tggagcagtt
     3541 ccgcgagacc gagctcgcgc ccggcgccgc cgtgcacctg cgtgccggta cggcctgcgc
     3601 cgactccgac acgccgttct ggggctacgg cattgcggat gccccgatcg tcgtggacgt
     3661 gtacgacggt catgagccgc cgacgttcgg tgaccgcacg gcgcgcgagg tgttccaggg
     3721 actggagatc cagggctgga agatcgcctt ccgcgacggg gagccgaccc tctcgctcgg
     3781 atccggcacg gtgaaggtcg gcgacgaggt cgtcgtctcg gtggcggact tccccgcgtc
     3841 ctcggaggta tccgtgggcg tcgagggcgc cggacgcccg cgtggcgcga tcacgacgga
     3901 ttcgcagggg gccggttcgg tcgtgctgcg gatcgctggg ctcgcccctg gcgcatatca
     3961 gctcatcgcg accggcccgg agggcacccg cgcctctggg acggtcgtgg tcgaggccgg
     4021 gcggagcgca gcgcaccggc aggacggcgg aatcgagatc gcgcccgcct gatcggggcg
     4081 gacggagggc gccggatgcc gggacagaaa ccgcatccgg tgcctgtcga tcgctgaagt
     4141 gatgcgcgtc agggcaggcc tcagatcacg gaggcctggc cgctgcaacc gtcaccgcct
     4201 ctcaggcgag ctgacgtcgc gccgcgtcgc acagccaggc cgtgtaccgc ggcatgctcc
     4261 acccggactg cagcacgagc tgctgatgcc cctcgggtga ccagaggaac gacagggcgg
     4321 ccgctgcgcc ggggatgtcg gtgtcggccg gggcgatccc tcggtcgatg agcagctgga
     4381 cggtggttcg gtagtcggcc atgcggcgtg cgagcagcgc ctcgagcgcc tcggcgatga
     4441 cggggtcggc gcctgcggcg ctgaggatct cgatccacag cacgctcgtc cgggcgttcg
     4501 cagcggcgat gaagatgaac acctggtcgg acatcgtcgt cccttccgtc gtgcggtgag
     4561 tcctccgatc ctgccaggga cggatgccgt gggccacccc tcccgccgac gcgaccgcct
     4621 cgccgtcagc c
//
LOCUS       DATMSX010000025         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_151960,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000025 DATMSX010000000
VERSION     DATMSX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151960"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VN041_00770"
     CDS             complement(<1..814)
                     /locus_tag="VN041_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWU27613.1"
                     /translation="MSALPFPASVYAARLERAGALAAEAGIDAVIVGPGPDLRYLLGV
                     EGDTIERLTALVLGPGFEPTIVVPRMELAKVRDTAAGELGVRIADWVDGEDPYALVVD
                     ALPASGGRIAVSDALPALHVIPIGDRMDARLELATPVLREGRMIKDADEVAELRRAGD
                     AIDAVHRRVADWLHAGRTEREVAADIAEAIRAEGHETVEFVIVGSGPNGADPHHEVSD
                     RVIEDGDVVVVDIGGAVPSGYNSDSTRTYVVGSPTAEQAERIAVLVRAQQAAV"
     gene            complement(855..1310)
                     /locus_tag="VN041_00775"
     CDS             complement(855..1310)
                     /locus_tag="VN041_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27614.1"
                     /translation="MAEQDKTVVFSDEERAAMKAASSERRTARSRAKKDPDEVRREGE
                     AEVRAKLADMPDDDRALGEALHAMVGEAAPHLVPRTYYGMPAYANEAGKVVCFYKPRS
                     KFKVRYSTFEFDQAANLDDGAIWPTAYAVTALGAEQLAFLAERVRIAAS"
     gene            1455..1877
                     /locus_tag="VN041_00780"
     CDS             1455..1877
                     /locus_tag="VN041_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU27615.1"
                     /translation="MDLRVAAYAVVTDDEDRILLARWTEGRRVAWTMPGGGLEDGEDP
                     EVAVRRELREETGFHIETTELLGIHSRVIPGSQRVHRERTPLHTIRIVYRAEITGGRL
                     RFEQNGSTDMAAWFTIPEVAELQRVKLVDIALRMAGII"
     gene            1911..3098
                     /locus_tag="VN041_00785"
     CDS             1911..3098
                     /locus_tag="VN041_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU27616.1"
                     /translation="MAEKLSPVSAVRNVRPWGGALSDILIDGATISQVVAASGAPIGD
                     GEIDGRGLLAIPGIVNTHAHIDKSWWGLPWQSYGGVRSTDGRIQHERAHRDELGIPSV
                     ELTSRVLAEFVKHGTTAIRSHVDVDLGLGQRGIEAVREAVAAYEGGIACEIVAFPQDG
                     VLRRPGVLELLADAAKAGAEHIGGLDPAGIDKDPVGQIDAIFALAADHGVGIDIHLHD
                     GGSLGAFQFDLIIDRTQTLGLQGRVNIAHGFAIVDADDARRHDLLQQMGELGISMTTV
                     APLRMKQFGLHEFADAGVRFGFGTDGIRDLWSPYGTGDTMGIAWQFARSSGVVYDDDL
                     RRVVELATSLGAPFAGVEKNDLTAGSRADVVLVDSENTMDVLVRTLPREVTVGGGKLL
                     HQR"
     gene            complement(3102..3587)
                     /locus_tag="VN041_00790"
     CDS             complement(3102..3587)
                     /locus_tag="VN041_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWU27617.1"
                     /translation="MGSEGHERVRAAASARGLEIEIRERPAARSLHEAAELLGLQPSD
                     IVKTLVVKRSDDTYLFALVPGGRSISWPKLRAIVGVNKLRLPEPELALAATGYERGTI
                     VPIGSTTDWPVFADQTIVGRRIAMGAGAHGYSLFVDADDLIRAYGATVADISEPEQPR
                     D"
     gene            complement(3665..4159)
                     /locus_tag="VN041_00795"
     CDS             complement(3665..4159)
                     /locus_tag="VN041_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HWU27618.1"
                     /translation="MNLSRKRKKELRKLQDDAGQLWEAQQVLVGHAADIAREAGRQLG
                     NFNREQVVPVVQDAYSRKVAPTVDRGLKIGKHVVDDKVVPIVGGVVGTALSAWDVANQ
                     KRYDLGIGHRPAPKKKTMGLGSVVALVLGTAAAIGVVVAAWQALRADDELWVADDPLS
                     TPNA"
     gene            4297..4854
                     /locus_tag="VN041_00800"
     CDS             4297..4854
                     /locus_tag="VN041_00800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU27619.1"
                     /translation="MPHASHVATLHTNHGDIVVNLFGDHAPNTVANFVGLADGTKDWT
                     HPATGKPGEGPLYKDVIFHRIIPNFMIQGGDPLGQGVGGPGYNFNDEINMELNFNEPY
                     ILAMANAGLRRNAITGQVEGTNGSQFFITTDPTPWLQGKHTIFGAVADEDSKAVVDAI
                     SAVPTAAGDRPIEPVVLQSIDIVQA"
     gene            4873..5043
                     /locus_tag="VN041_00805"
                     /pseudo
     CDS             4873..5043
                     /locus_tag="VN041_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            complement(5160..5630)
                     /locus_tag="VN041_00810"
                     /pseudo
     CDS             complement(5160..5630)
                     /locus_tag="VN041_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
BASE COUNT          942 a         2033 c         1811 g         1029 t
ORIGIN      
        1 ccaccgcggc ctgctgcgcg cggacgagca ccgcgatgcg ctcggcctgc tccgcggtgg
       61 gcgaaccgac cacatacgtg cgcgtgctgt cggagttgta gccgctcggc accgccccgc
      121 cgatgtcgac cacgaccacg tcgccgtcct cgatcacccg atccgagacc tcgtggtgcg
      181 gatcggcacc gttcgggccg gagccgacga tcacgaactc cacggtctcg tgcccctcag
      241 ccctgatcgc ctcggcgatg tcggccgcca cctcacgctc ggtgcgcccc gcgtgcagcc
      301 aatccgccac ccgacggtgc acggcgtcga tggcgtcccc ggcccggcgc agctccgcga
      361 cctcgtcggc gtccttgatc atgcgaccct cgcgcagcac gggcgtcgcc agctccagcc
      421 gggcgtccat ccgatccccg atcgggatca cgtgcagtgc ggggagggca tccgacaccg
      481 cgatcctccc gcccgacgcg ggcagcgcgt cgacgaccag cgcataggga tcctcgccgt
      541 ccacccagtc ggcgatccgc acgcccagct cgcccgccgc ggtgtcgcgc accttggcga
      601 gctccatgcg cggcacgacg atcgtcggct cgaacccggg acccagcacc agggccgtga
      661 ggcgctcgat cgtgtcgccc tccacgccca gcaggtagcg caggtcgggg ccgggaccca
      721 cgatcacggc gtcgatgccg gcttcggcgg ccagggctcc ggcccgctcg aggcgggcgg
      781 cgtagacgga tgcggggaag ggaagagcac tcacgcgctc cacgctaccg ccgcatccgc
      841 acgcaggaca ggtgtcagga tgcggcgatc cgcacgcgct cggccaggaa ggcgagctgc
      901 tcggcgccca gcgcggtgac cgcatacgcg gtgggccaga tcgccccgtc gtcgaggttc
      961 gcggcctggt cgaactcgaa ggttgagtag cgcaccttga acttggagcg cggcttgtag
     1021 aagcacacca ccttgccggc ttcgttcgcg tacgccggca tgccgtaata cgtgcgcggc
     1081 accagatgcg gggcggcctc cccgaccatc gcatgcagcg cctctccgag cgcccggtcg
     1141 tcgtccggca tgtcggccag cttcgcgcgg acctcggcct cgccctcccg gcggacctcg
     1201 tccggatcct tcttcgcccg cgaacgggcc gtgcggcgct cgctcgacgc cgccttcatc
     1261 gccgcgcgct cctcgtccga gaacaccacg gtcttgtcct gctcagccat gtcggcctcc
     1321 ttcgtgtctg atcccacgct agaagccacg tcgtcaccgc gcttctcgat tcctgatcgg
     1381 atgccgtggg ccgcccggac tccgcatggc gggacgcccg ggatgcacgc gggaaccggc
     1441 gctactgtga atccatggat ctgcgcgtcg ccgcctacgc cgtcgtcacc gacgatgagg
     1501 accgcatcct gctcgcacgc tggaccgagg gacgccgcgt cgcctggacc atgccgggcg
     1561 gaggactcga ggacggcgag gaccccgagg tcgccgtgcg gcgcgagctg cgtgaggaga
     1621 ccggcttcca catcgagacg accgagctgc tcggcatcca ctcccgggtc atccccggct
     1681 ctcagcgcgt gcaccgggag cgcacaccgc tgcacaccat ccgcatcgtg taccgggccg
     1741 agatcacggg cggtcggctg cgcttcgagc agaacgggtc caccgacatg gcggcgtggt
     1801 tcacgatccc cgaggtggcg gagctgcagc gcgtgaagct cgtggacatc gcgttgcgaa
     1861 tggccggaat catctagtca tcagacaacc ggatcggcga gaatggggcc atggccgaaa
     1921 agctctcccc cgtctccgcc gtccgaaacg tccgtccctg gggcggtgcg ctcagcgaca
     1981 tcctcatcga cggcgccacg atctcgcagg tcgtcgcggc gtccggcgct ccgatcggcg
     2041 acggcgagat cgacggccgc ggcctgctcg ccatccccgg catcgtcaac acccacgccc
     2101 acatcgacaa gtcgtggtgg ggcctgccgt ggcagtccta cggcggcgtc cgcagcaccg
     2161 acggacgcat ccagcacgag cgcgcgcacc gggacgagct cggcatcccc agcgtcgagc
     2221 tcacctcgcg cgtgctggcc gaattcgtca agcacggcac caccgccatc cgcagccatg
     2281 tcgatgtcga cctcggcctc ggtcagcgcg gcatcgaggc cgtgcgcgag gccgtcgccg
     2341 cctatgaggg cggaatcgcc tgcgagatcg tcgcgttccc gcaggacggc gtgctgcgcc
     2401 gcccgggggt gctggagctg ctcgcggatg ccgcgaaggc cggcgccgag cacatcggcg
     2461 gcctcgaccc ggccggcatc gacaaggacc cggtcggaca gatcgacgcg atcttcgccc
     2521 tcgccgccga tcacggtgtc ggcatcgaca tccacctgca cgatggcgga tcgctgggcg
     2581 ccttccagtt cgacctcatc atcgaccgca cccagacgct cggtctgcag ggccgcgtca
     2641 acatcgcgca cggcttcgcg atcgtggatg ccgacgacgc gcgccgccac gatctgctgc
     2701 agcagatggg tgagctcggc atctccatga ccaccgtcgc gccgctgcgg atgaagcagt
     2761 tcggcctgca cgagttcgcc gatgccggcg tgcgcttcgg cttcggcacc gacggcatcc
     2821 gcgacctgtg gagcccctac ggcaccggtg acaccatggg catcgcctgg cagttcgcgc
     2881 gcagctccgg cgtcgtctac gacgacgacc ttcgccgcgt cgtcgagctg gcgacctcgc
     2941 tgggcgcgcc gttcgcgggc gtggagaaga acgacctgac cgccggatcg cgagcggatg
     3001 tcgtgctcgt cgactccgag aacaccatgg acgtgctcgt gcgcacgctc ccccgcgagg
     3061 tcaccgtggg tggcgggaag ctgctccacc agcgctgacg gtcagtcgcg gggctgttcg
     3121 ggctcggaga tgtcggcgac cgtggcgccg taggcgcgga tgaggtcgtc ggcgtccacg
     3181 aacaggctgt acccgtgtgc gccggcgccc atggcgatgc gacggccgac gatggtctgg
     3241 tcggcgaaga ccggccagtc ggtggtgctg ccgatgggca cgatcgtccc gcgctcgtag
     3301 cccgtcgcgg ccagcgccag ctcgggttcg ggcagccgca gcttgttcac gccgacgatc
     3361 gcgcgcagct tgggccaaga gatcgaccgc ccgccgggca cgagggcgaa caggtaggtg
     3421 tcgtccgagc gcttgaccac cagcgtcttg acgatgtcgg agggctgcag accgagcagc
     3481 tcggccgcct cgtgcaggct gcgggcagca ggacgctcgc ggatctcgat ctcgagtccg
     3541 cgagcggatg ctgccgcccg gacccgctcg tgcccttcgc tccccacgcc ccactccgat
     3601 cgtcgcttcg cgccgaccgg agcctccggg gcgcgtgggc ccggctcagg ggccgagggg
     3661 gtggtcacgc gttcggcgtc gacagcgggt cgtccgcgac ccacagctcg tcatctgcgc
     3721 gcagcgcctg ccaagcggcc acgaccacgc cgatcgccgc agcggtgccg aggaccagtg
     3781 ccacgacgga gccgagtccc atggtcttct tcttcggtgc cgggcggtgc ccgatgccca
     3841 ggtcgtagcg cttctgattg gcgacgtccc acgccgacag cgcggtgccg acgacgccgc
     3901 ccacgatcgg gacgaccttg tcgtcgacga cgtgcttgcc gatcttcagc ccgcggtcga
     3961 ccgtcggggc gaccttgcgg ctgtaggcgt cctgcacgac cggcacgacc tgctcgcggt
     4021 tgaagttgcc gagctgccgg cccgcctcgc gggcgatgtc cgcggcgtgg ccgacgagca
     4081 cctgctgcgc ctcccacagc tgtcctgcat catcctgaag cttgcgcagt tccttcttgc
     4141 gcttgcggct gaggttcacg ttgctctccc gtcggtcaga gtccgttcgt gttcatcttg
     4201 ccatgcgagg ctggatgcgg gcagggatct ggcgctgacg gcgaacatct gcgatcatgc
     4261 cagcccggtc agaggtcgct ctgcgagaat gtcaccatgc ctcacgcttc tcacgtcgca
     4321 accctgcaca ccaaccacgg cgacatcgtc gtcaacctgt tcggcgacca cgccccgaac
     4381 acggtcgcca acttcgtcgg cctggccgac ggcaccaagg actggacgca ccccgccacg
     4441 ggcaagcccg gcgagggccc gctgtacaag gacgtcatct tccaccgcat catccccaac
     4501 ttcatgatcc agggtggcga cccgctcggt cagggcgtcg gcggccccgg ctacaacttc
     4561 aacgacgaga tcaacatgga gctgaacttc aacgagccct acatcctcgc catggcgaac
     4621 gccggcctgc gccgcaacgc catcaccgga caggtcgagg gcaccaacgg ctcgcagttc
     4681 ttcatcacca ccgacccgac cccctggctg cagggcaagc acacgatctt cggcgccgtc
     4741 gccgacgagg actccaaggc cgtcgtcgac gccatctcgg ccgtgccgac cgccgcgggc
     4801 gaccgcccga tcgagccggt cgtgctgcag tcgatcgaca tcgtccaggc ctgagagcgc
     4861 gagccgatcc ggatgacgaa cccagagttc acgagcaatc gcgacaactt ctgctaccgg
     4921 catccggatc ggcagagctt cgtgctctgc cagcgctgca tgcgcaccat ctgcccggaa
     4981 tgccagacgc agggacccgt cggggtcatc tgcccggagt gcatgaagga gcagcgcaag
     5041 gcggagtccc ccgcccagcg ccgcgcccgt cgcgcctggg gccgcggtcc ggtcgcgatg
     5101 tgtttcataa ttctgtggac aattgatagc ccgactccat ttcctttgaa ttttttggca
     5161 ttatccatcc tgttgaaaat tttaaacatt ttgtgtttat tttcttcagg aagaccgatt
     5221 ccgttgtctg aaattctgta gatcaccatc tcgccctgtt cggtcccttc aatctccacc
     5281 accggttttt cctgttggga agagtatttt attgcgttgt tgatgatgtt caaaaacacc
     5341 tgatggagca ttgttttatc agcaagaact tccggacatg ccttaatgat aacttcactt
     5401 ttcggagttc cgaaagtaat tttggcattt tcacaaatct tttcaatggt ttgcaccgtt
     5461 tcaagttttt caagctggat ttcactgtgt ttagcacggc tgagctgtaa aacatcatgc
     5521 atcatttccg ccatattgtc tatctcttcg atgatagaat taattttatt acggcttttc
     5581 tcagattcgt ctattaaagt tttaagaagc atctgtgcat tcagcttcat caccgtaagc
     5641 ggtgttccca ggtcatgaga aatagtatat gaaaaactgt cgagttcttc atttactttt
     5701 ttaagctcgt cgttgagcct tttaatggta ttatagtctt tgtgtaatgc ttctaaaata
     5761 aggtcccgta ctccctgaag cgccagaata ttcctggaac tccatctttt agaac
//
LOCUS       DATMSX010000026         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19322,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000026 DATMSX010000000
VERSION     DATMSX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19322"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1112)
                     /locus_tag="VN041_00815"
     CDS             complement(213..1112)
                     /locus_tag="VN041_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU27620.1"
                     /translation="MTSTTIAGDARPAVAPRRRIKRKTVQTVVWFIGLSILTVIVLYP
                     LLWLFLATFKPNAEFGTNPGLFPNQPTVDNYGKVMEGIAGVPMWRFFLNSFILAVSAV
                     VGTVLSSALAAYAFARVQFKGLGIFFAAMIGTLLLPFHVVIIPQYIIFNKLGWVDTFI
                     PLILPKFLATSAFFVFLIVQFIRQMPRDMDEAARIDGAGHLRIFWSIILPLIKPALIT
                     CAIFAFIWEWNDFLSPLLYLTSPENYPLPIGLRLYNDASSGGADYGATVTASFVALIP
                     ILLFFIVFQRFLVDGVATQGLKG"
     gene            complement(1109..2104)
                     /locus_tag="VN041_00820"
     CDS             complement(1109..2104)
                     /locus_tag="VN041_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU27621.1"
                     /translation="MSTTATRVIVTEDRSSAHLLRKSRREVDPVRPGQRARVRRETAA
                     GYAFLTPWLIGFLGLTLVPMVYSLYLSFTRYNIFAPPKWIGLDNYIRLFTKDPVFLQS
                     SEITLIYVFVGTPITLAAALGTAMLLNYRDKGSGFFRSAFYAPSLIGASVSIAIVWRA
                     MFATDGPVDSGLQVFGIDLGGWIGNPSMVLPMMILLHVWTFGSTMVIFLAGLKQIPKE
                     LYEAAEMDGAGAWRRFRAVTIPMLSPVMFFNLLLGLIGAFQVFASAYIISNGSGGPAG
                     MTNFITVYLYKRGFSDGQMGYAAAMAWVLLIVVALLAFILFRTQKSWVHYAGDDK"
     gene            complement(2189..3484)
                     /locus_tag="VN041_00825"
     CDS             complement(2189..3484)
                     /locus_tag="VN041_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU27622.1"
                     /translation="MFKKRLAAGTALATAAVLLLAGCSGQAADPKAKATFDPKEKVTL
                     NLAFWGNDVRADLYNQVIAEFNKKYPNIKVNSTFLGFPEFWEKRQTEAAGGGLPDVMQ
                     FDYSYLRQYSENGLLLDLDPYIGGIIDKKPLADNILKIGVVDDKTYAIPTSTNAWGLF
                     TNPKLLETAGVSDFEGGSWSDYADWMKSVTDASGGAFYGGSDYTGRIQNFEIQLRSEG
                     KNLFNEDGTAGFDEKRLAKFWNEGAAVRKGVGIPQQKVEELTPLGAFDSAKTASELTW
                     DNFGAGYLKNLGADYTELNLVAPPVTKKGTKDLYLKPSMLHAISAKTKHPEAAATLVN
                     FLINSPESGKIFGTNRGLPASSTALAAADLDPLSQQIKDYETSIKDRLGDAPPVPIVG
                     YGSLEEKFRVLGTELNFGTITVDDAVKQFFAEMDVVLNQ"
     gene            complement(3663..4055)
                     /locus_tag="VN041_00830"
     CDS             complement(3663..4055)
                     /locus_tag="VN041_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27623.1"
                     /translation="MTVVATSPDLRAWRIDGVAVGGRPHEGPDVFELGGFWWMIVDEW
                     RGMGVHRSGDGIRWVRQGGADAVILDALPAGGHGHHGAHVRAGDDVWFYFFGRAADAE
                     PHAQEEDGRASVVHRVRLDVVDGVLVAR"
     gene            complement(4221..4601)
                     /locus_tag="VN041_00835"
     CDS             complement(4221..4601)
                     /locus_tag="VN041_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27624.1"
                     /translation="MIPGSVGPGRLPELFRDPVYDGATDPRVVIDDDGRWWMLYTQRR
                     ANLEDAGSGVAWVHGSRIGVAVSDDGVRWRYEGTLEPSASGLFLTGGPPPASVDRTHW
                     APEVVHDGELWRMYLTEIDGIPDR"
     gene            complement(4598..>5257)
                     /locus_tag="VN041_00840"
     CDS             complement(4598..>5257)
                     /locus_tag="VN041_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU27625.1"
                     /translation="ALGRPLVAVCQTLWFRDADYFAVPWRGRWETEGGGTTLGHGIHQ
                     LDLLAHLLGDWSSVQGRLFRLDRETQTEDVSTATIVFEAGVVAQVITSAISPRETSSI
                     RIDTQKATVTVDHLYGHGHENWRITPAPGFESEAEGWAFPDVEERSDHAPLLRDVFDA
                     LLDGKPLPPTADAPARSLELVAAIYASASADGAVITPEILATHPTHRAGFASPVIDLR
                     A"
BASE COUNT          874 a         1704 c         1842 g          837 t
ORIGIN      
        1 gatgcccgcg gcgagggcga tcggcagcgt gaccaccgta agtgcgacca gcgtgatcag
       61 caggccgacc gtgagggcct cgccgaacag gccgaacttc gccgtcgcgc ccgggtgggg
      121 gccggggtcg aatccggctc cgaccgggcc cgagccggag cgcgcctcac gcgccacggc
      181 cctcgcctcg cgtcgccctg ccgcggtcat cgtcagccct tcagtccctg cgtggcgacg
      241 ccgtcgacca ggaaccgctg gaagacgatg aagaacagca ggatcgggat gagggcgacg
      301 aacgacgcgg tgacggtggc gccgtagtcc gcgccgccgg aggaggcgtc gttgtacagc
      361 cgcagaccga tcgggagcgg gtagttctcg gggctggtca ggtagagcag cgggctgagg
      421 aagtcgttcc actcccagat gaacgcgaag atcgcgcagg tgatcagagc cggcttgatc
      481 agcggcagga tgatcgacca gaagatgcgc agatgacctg cgccgtcgat gcgggcggcc
      541 tcgtccatgt cgcgcggcat ctggcggatg aactgcacga tcaggaacac gaagaacgcg
      601 ctcgtggcga ggaacttcgg caggatcagc gggatgaacg tgtccaccca gccgagcttg
      661 ttgaagatga tgtactgcgg gatgatcacg acgtggaacg gcagcaggag cgtgccgatc
      721 atcgcggcga agaagatccc cagccccttg aactgcacgc gggcgaacgc gtacgcggcc
      781 agcgccgacg agagcacggt gccgaccacg gccgagacgg cgaggatgaa cgagttcagg
      841 aagaaccgcc acatcggcac gcccgcgatg ccctccatga ccttgccgta gttgtcgacg
      901 gtgggctggt tggggaacag accggggttc gtgccgaact cggcgttcgg cttgaaggtg
      961 gccaggaaca gccacagcag cgggtagagg acgatgacgg tcaggatgct gaggccgatg
     1021 aaccagacga cggtctggac ggtcttgcgc ttgatccggc gccgcggcgc gaccgccggg
     1081 cgggcgtcgc cggcgatggt ggtggaggtc atttgtcgtc tcctgcgtag tgcacccagg
     1141 acttctgcgt gcggaagagg atgaatgcca gcagcgcgac cacgatcagc agcacccagg
     1201 ccatggcggc ggcgtagccc atctgcccgt cggagaatcc tcgcttgtag aggtagacgg
     1261 tgatgaagtt cgtcatgccc gccgggccgc ccgacccgtt ggagatgatg tacgcggatg
     1321 cgaacacctg gaacgcgccg atcaggccga gcagcaggtt gaagaacatc accggcgaga
     1381 gcatcgggat ggtcaccgcg cggaatcgac gccaggcgcc ggcgccgtcc atctcggccg
     1441 cctcgtacag ctccttcggg atctgcttga gaccggcgag gaagatgacc atcgtcgacc
     1501 cgaaggtcca cacgtgcagc aggatcatca tcggcagcac catcgacggg ttgccgatcc
     1561 atccgcccag gtcgatgccg aacacctgca ggccggagtc gacgggaccg tcggtggcga
     1621 acatcgctcg ccacacgatc gcgatcgaga ccgatgcgcc gatcagggac ggcgcgtaga
     1681 acgccgagcg gaagaacccg gagcccttgt cgcggtagtt cagcagcatc gccgtcccga
     1741 gcgccgcggc cagcgtgatc ggagtgccca cgaacacgta gatcagcgtg atctcggacg
     1801 actgcaggaa caccgggtcc ttcgtgaaca gccggatgta gttgtccagg ccgatccact
     1861 tcggcggggc gaagatgttg taccgcgtga acgacaggta cagcgagtac accatcggca
     1921 cgagggtgag ccccaggaat ccgatcagcc acggggtgag gaaggcgtat cccgccgcgg
     1981 tctcacggcg gacacgggcg cgctggccgg ggcggacggg gtccacctcg cggcggctct
     2041 tgcgcagcag atgggcgctg gagcggtctt cggtgacgat gaccctggtc gcggtggtgc
     2101 tcacggggtc ctccttcggg gttcgtgact gacggcggcg ggagtgcggc cggcgggccg
     2161 gatgctggat ccggcccgcc gccggatctc actggttgag gacgacgtcc atctcggcga
     2221 agaactgctt cacggcgtca tccaccgtga tggtcccgaa gttgagctcg gtgcccagca
     2281 cgcggaactt ctcctcgagg ctgccgtagc cgacgatcgg cacgggcggg gcgtcgccga
     2341 gacggtcctt gatggaggtc tcgtagtcct tgatctgctg gctcagcggg tcgaggtcgg
     2401 ccgcggcgag cgccgtggac gacgcgggca gaccgcggtt ggtgccgaag atcttgccgg
     2461 actccggcga gttgatcagg aagttcacca gcgtcgcggc cgcctcgggg tgcttggtct
     2521 tcgccgagat ggcgtgcagc atggagggct tcaggtacag gtccttcgtg cccttcttgg
     2581 tgaccggcgg ggcgaccagg ttcagctcgg tgtagtccgc gccgaggttc ttcaggtatc
     2641 cggcaccgaa gttgtcccag gtcagctcgc tggccgtctt ggcggagtcg aacgcgccga
     2701 ggggcgtgag ctcctcgacc ttctgctgag ggatgccgac gcccttgcgc acggcggcgc
     2761 cctcgttcca gaacttggcg aggcgcttct cgtcgaagcc tgcggtgccg tcctcgttga
     2821 agaggttctt gccctcggag cgcagctgga tctcgaagtt ctggatgcgt ccggtgtagt
     2881 cgctaccgcc gtagaaggcg ccgcccgagg cgtcggtgac cgacttcatc cagtcggcgt
     2941 agtcgctcca gctgccgccc tcgaagtcgg agaccccagc ggtctcgagc agcttggggt
     3001 tagtgaacaa gccccaggcg ttggtcgacg tcgggatcgc gtaggtcttg tcgtcgacga
     3061 cgccgatctt gaggatgttg tcggcgagcg gcttcttgtc gatgatgccg ccgatgtacg
     3121 ggtcgaggtc gagcagcagg ccgttctcgg agtactgacg caggtacgag tagtcgaact
     3181 gcatgacgtc gggcaggcca ccgccggcgg cctcggtctg acgcttctcc cagaactcgg
     3241 ggaagccgag gaaggtcgag ttgaccttga tgttcgggta cttcttgttg aactccgcga
     3301 tcacctggtt gtacaggtcg gcgcggacgt cgttgcccca gaaggcgagg ttgagcgtga
     3361 ccttctcctt cgggtcgaag gtggccttcg ccttgggatc ggccgcctgc ccggagcagc
     3421 cggcgagcag cagcacagcc gctgtggcga gggcggttcc ggccgccagt cgcttcttga
     3481 acatcgttgt cctctctgtg gacgtcttcg tcgggtcgag ccgttgaacg tccatgtcca
     3541 tgtgggtgcg ggtgcgcctc cagggactct cggaaagcgc ttccctgcaa actaaccaaa
     3601 attgaaacgt ttcgcaagac ccaggccgga atctgttcta caacgtttcc cttatgtgat
     3661 cttcatcgtg ccacgagaac gccgtcgacg acgtccagtc gcactcggtg caccaccgac
     3721 gcacgcccgt cctcctcctg agcatgcggc tcggcatccg ccgcccggcc gaagaagtag
     3781 aaccagacgt cgtcgcccgc tcgcacgtgc gcgccgtgat gaccgtgccc accggccggc
     3841 agcgcgtcca ggatcacggc atccgcgccg ccctgccgca cccagcggat gccgtcgccc
     3901 gagcggtgca cgcccatccc ccgccactcg tccacgatca tccaccagaa gccgccgagc
     3961 tcgaacacgt cggggccttc gtggggacgt ccgccgacgg cgaccccgtc gatgcgccac
     4021 gcgcgcagat cgggcgaggt ggcgacgacg gtcaccgagt ccctggcctc gtccttgcac
     4081 cacagccgcc agagaccgtc cggcgtccgc gcgaccgcgg catcgatgac ccgatcgctg
     4141 ccgagcgcca gcggtccgat ccgcctccat gcccgcaggt cgtcggagac gtactcgacg
     4201 atcgtgcggg cgtgccccgg tcaacgatcc gggatgccgt cgatctcggt caagtacatg
     4261 cgccacagct cgccgtcatg gacgacctcc ggggcccagt gggtgcggtc cacggatgcc
     4321 ggcggtgggc cgcccgtgag aaacagaccc gacgccgagg gctcgagtgt cccttcgtac
     4381 cgccatctca cgccgtcgtc ggagactgcg acgccgatgc ggctgccgtg aacccacgcg
     4441 acccccgacc cggcatcctc gaggttggct cggcgctggg tgtacagcat ccaccagcga
     4501 ccgtcgtcgt cgatgaccac acgcgggtcg gtggcgccgt cgtagacggg gtcgcggaac
     4561 agctcgggca ggcgcccggg tccgacggac ccggggatca tgcccgcagg tcgatgacgg
     4621 ggctggcgaa gcccgcgcgg tgcgtcggat gcgtagccag gatctcgggc gtgatcaccg
     4681 cgccgtcggc cgaggccgag gcgtagatgg cggccaccag ttcgagggag cgcgcgggcg
     4741 cgtccgccgt gggcggcagc ggcttgccgt cgagcagggc gtcgaacacg tcgcgcagca
     4801 gcggcgcgtg gtcgctgcgc tcctccacat cggggaacgc ccatccttcc gcctcggact
     4861 cgaatccggg agccggggtg atgcgccagt tctcgtgacc atgcccgtag aggtggtcga
     4921 cggtgaccgt cgccttctgc gtgtcgatgc ggatcgagct ggtctcgcgc ggcgagatcg
     4981 cgctggtgat cacctgcgcg acgacgcccg cctcgaacac gatcgtggcg gtggagacgt
     5041 cctcggtctg ggtctcgcga tccagccgga acagccgccc ctgcaccgag gaccagtcgc
     5101 ccagcaggtg cgcgagcagg tcgagctgat ggatgccgtg gccaagggtc gtcccgccgc
     5161 cctcggtctc ccacctgccg cgccacggca cggcgaagta gtcggcgtcg cggaaccaca
     5221 gcgtctggca cacggcgacc agcggccgcc cgagcgc
//
LOCUS       DATMSX010000027        22010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_152021,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000027 DATMSX010000000
VERSION     DATMSX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 22010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22010
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152021"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VN041_00845"
     CDS             <1..883
                     /locus_tag="VN041_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU27626.1"
                     /translation="DLLKGKRFDLVVANDARALPVAFAAAHGAPVWADLHEWAPEEQS
                     HILSWRLLVAPFMTYLCGIYLPRCAAVSTVSPLIAELYEKEFGAAADVVRNARDFVEL
                     SPSPLQPGRIRLVHSGVAVPERCIETLIDAMHDLDDRFSLDFFLVFGDDDSYRRQLVA
                     RAAGSDRITFHDPVAPHELPAALNGFDLGVYLLRPTTTNHRFMLPNKFFDFVQSRLGV
                     VFGRAVEIDALIDSHELGLTVDGFEPADLVAALSALTEDDVRAFKQHADAAAHELSSD
                     VDAEVQRGIIRRSLNRG"
     gene            complement(896..2674)
                     /locus_tag="VN041_00850"
     CDS             complement(896..2674)
                     /locus_tag="VN041_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27627.1"
                     /translation="MKSVIALYREVTSVLPPEARRFIATYSWLLASLAIFDAAALGLL
                     ALVIGPLAAGTPVRLPLIGELDGMGVIIAILLICAMMIAKGLLSTLVMWWGTRRIARY
                     QVVVGDRLFRAYIHAPWTLRLKKNSSDMLRFTDGGVDAMINSFLLPGATLLGEAVSLF
                     VVVATLALVQPAIAAITLVYILLLGAVLYLWIAKHARVAGEVNLAASIRTSRLILEIM
                     STLKEISLRNKEDAVADVVTKSRTESSRARANTYFLGQVPRYVLESGIIGGFIVVGGA
                     GFLIGGQEAAITAVAMFGLAGFRMAPSITRFQSIMSMMQSTAPYPRKVLDELAEAEQQ
                     IATAPGGGDGTVPEAPKTLRLDNVSYRYSPEAALAVRDVSIDIPFGSFVAFVGASGSG
                     KSTMVDLILSLLEPTQGVISVDDAPLTSMARSWRERVGYVPQDVALFDATIGRNVALT
                     WDDDVDAARVEMALRQAHVWDIVSRRDGGIDSQVGERGISLSGGQRQRLGIARALYVD
                     PLVLVMDEATSALDTKTEAAVSESITSLAGQRTLIVVAHRLSTIRHADQIFFMRDGEV
                     AGRGTFDELVAAVPDFAHQAALAGLA"
     gene            2809..3966
                     /locus_tag="VN041_00855"
     CDS             2809..3966
                     /locus_tag="VN041_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWU27628.1"
                     /translation="MRIVIVSRIFTPEPAAASFLLRATAEAFRDAGHEVEVLTSTPPP
                     AASGADPRGIRVRRARVKRDRQGYVRGYLSYLSFDIPVLFRLLFSRRADLYLVEPPPT
                     TGAVVRAVSLLRRTPYVYDAADLWADAAGVVTSSPLVLGALRAVERFAMRGTAHAFAI
                     SEGLIDRMRQIGIDVPATAVGFGTDTASFRYAPQDPPTAPLFVYPGSYSEWHGADVFL
                     EAFAGVREAHPDARLLFIGNGSEQPRMRARIAELGLTGVEFRDPVPPEQLADVLSSAT
                     ASLASLKPGAGYDYAFTTKVYGSLVSGCPVIFAGAGPTGPFLRTESQHHPVGTAVEWD
                     AAAARAAMLAAAEEPLATSRRVELAVWAAGRYSLSAVAERIVERSEEIVRG"
     gene            4010..5305
                     /locus_tag="VN041_00860"
     CDS             4010..5305
                     /locus_tag="VN041_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWU27629.1"
                     /translation="MKIAVVALGKIGLPLAVQFASSGHDVIGVDVNQRAVDTINAAVE
                     PFPGEAHLQEKLEELIPTGRLRATTDYSEAIPGADAVVLVAPVFVNDETWEPDFKYMD
                     AATKSLAEHLTPGTLVSYETTLPVGTTRTRWKPMLEEGSGLSEGVDFHVVYSPERVLT
                     GRVFADLRKYPKLIGALSDEGTRRAREFYEAVLQFDERPDLAQPNGVWDLGSTEASEM
                     AKLAETTYRDVNIGLANQFGVFAASHGIDVYKVIEACNSQPYSHIHRPGIAVGGHCIP
                     VYPRLYLSTDPDADIVRVARQFNASMPERLVAQAAGVLGDLTGRRAVVLGAAYRGGVK
                     ETAFSGVFPTVAALKQRGAEVFVHDPLYTDDELRGLGFEPFALGAEAELAVLQTDHAD
                     YKTVSPEQIPGIKLLVDGRNATDAALWAGTPRIVVGAAA"
     gene            5310..6506
                     /locus_tag="VN041_00865"
     CDS             5310..6506
                     /locus_tag="VN041_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27630.1"
                     /translation="MISDALRRLATSIFVALPYQVADRIHPWRRRAFRDTSVTTVASD
                     RCTLLIGPVNSAGQGYAWARAAEHLPGVTAVNLMYRSSSDRFSFPADHSVDSAVASGV
                     RRWQNAEKAAILKSFTHVIVESGAHLFDTHAPVLHHLLRMKDADVEVALLFHGSDIRS
                     PRAHAQTEADSPFRDGQYEDTSLLESIASRNRVLIEAAGLPVFVSTPDLLLDVPEATW
                     LPVAVRPELWSAGATQVALERRRPIVVHAPSRAGLKGSTLIEETVRRLHDEGVIEYRE
                     VRGVPSSEMPRVYGESDVVLDQFSLGIYGVAACEAMAAGRLVISHVSDFVRREVRKRT
                     GLDLPIVEATASNLESVLRRVADSPSEYAAVTSAGAEFVRTVHDGSRSADALRAFLHR
                     SETTVE"
     gene            6503..8632
                     /locus_tag="VN041_00870"
     CDS             6503..8632
                     /locus_tag="VN041_00870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWU27631.1"
                     /translation="MSVISSGQTGVVSERRSTAAARSGRTAGFRPDVQALRAVAILAV
                     VLNHLWPARVTGGYVGVDVFFVISGYLITAHLLKEISSSGRIALAPFYARRARRLLPA
                     AFLVLAVSAAASIALLPYPRWARTGAEVLASAAYVENWFLSGMSVNYSALNDLATPVQ
                     HYWSLSVEEQFYLLWPLLLIGSWWLSSRLSSNPRRAAGVTLGVLVVVSLGLSVHATAL
                     APNQAYFVTHARAWEFGIGGLVALLPAARLSRPVAEVLSLSGFATIAFAVVTFDSLTP
                     FPGWTALLPVMGSAAVIIGGTGRTRLLHTPVTSTRPVQWLGDISYSLYLWHWPLMVLT
                     PFLLSGTLTTWMKLGVLATALIVSDLTRRLVEKPGQRWPMLRDSTRRTFLAMVLAMLL
                     VAALTVSMIIAGKVRTDADIPPKTMTIEACTGPQALDAASSCDDPFGPARESVMGPVN
                     AYFYTPPGCVGVPDLLRFGGTTATVRCDFSAGRESPTEVWLVGDSHAEQFKGPIIDIA
                     RKQGWVLTTSFGGGCPVIDAPFEGFRTQASPADKERCRTWSRAVQDAIALAAPDLVFT
                     SSAARVQMLDDGTGRTQSAQFTDGLERTWRRWISAGSTVVALGGVPFNGEVRSPDCVV
                     LNREAPRACAVPVEVANPPDPYLAAAVELADSRAIAFDASPYFCDQEQCFAVVGGIAV
                     YYDADHLNLDYARRLAPELMAVVERVL"
     gene            complement(8678..10753)
                     /locus_tag="VN041_00875"
     CDS             complement(8678..10753)
                     /locus_tag="VN041_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWU27632.1"
                     /translation="MNSKVMPPYRHALNRRWDFVMFKRAVVVVSTVVLALSTLTTASP
                     ALAATTAAVDLPGLLAIAPVDTNHAYSRDAFPHWIDADADGCNTRYEVLMQESTTPVT
                     IAPPCTITGGTWVSPYDGFATTDVTQIQIDHVVALAEAWRSGAWAWSTAQRQAFANDL
                     DVPYALVAASNSANQSKADNDPAGWMPSNTSYSCEYAISWALIKYRWSLSVDAAELST
                     LQTALQGECGTTEVQLPSIMVRSSPFHPELDPTVLFPPGVTRLQGKNRYETAVEVSRR
                     YTAGVKAVFVATGQDFPDALSAAAAAARLGGPLLLTPPTALPQVVREELHRLAPERIF
                     IAGGVGAVSAAVENDLRRITSDVSRLGGASRYDTGASIVESTFTAASHVFVATGRTFP
                     DALAATGAAGAAAEPVILVDGSLSSLTPTTSRLISRLGAESVTIVGGPGAVSAGIERQ
                     LSSSYDTTRLGGADRYATAARINDAHFPAGSAPAAFLATGADFPDALAGAALAGKLKS
                     PVYVTTSRCVPEPVHVSMGILGAPSSIALGGTAIVSDAAASNLGCLTAAVPVIDGAAR
                     AGSTLTVRTGAWTAGTSLQYQWLANGAAVSGATGSTFAITSSAVGKQISVRVTGSASG
                     YLTAVATSAKTAVVLAAAPEPPRYSGYVTAGAYCASQYAGWIGHTVTGLRMQCKTSTT
                     DSRLRWRAA"
     gene            10853..12001
                     /gene="glf"
                     /locus_tag="VN041_00880"
     CDS             10853..12001
                     /gene="glf"
                     /locus_tag="VN041_00880"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HWU27633.1"
                     /translation="MDLLVVGSGFFGLTIAERAAEAGRKVTVIDRRSHIGGNAYSEAE
                     PETGIEVHRYGAHLFHTSNATVWEYVNRFTTFTNYVHRVYTNHNSVVYPMPVNLGTIN
                     QFFQASYSPDEARALVKEQAGEFDVRTAENFEEKGIALVGRPLFEAFFRDYTAKQWQT
                     DPHKLSGDIISRLPVRYTYDNRYFNDTWEGLPTDGYTAWLERMADHPNIEVKLGVDYF
                     DESQPLNKRATVGQLPVVYTGPVDRYFDYAEGALSWRTLDFEQEVLNVGDFQGTSVMN
                     YPDLDVPFTRIHEFKHFHPERKDVFNSDKTVIMREFSRFANREDEPYYPVNTPADRDG
                     LLAYRELAKGEQDVHFGGRLGTYQYLDMHMAIGSALSMWRNELSGQDD"
     gene            12009..12827
                     /locus_tag="VN041_00885"
     CDS             12009..12827
                     /locus_tag="VN041_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27634.1"
                     /translation="MSRATVGAPGTPQRYAHSLWLLSARDLKVRYSTSALGYLWSVLD
                     PLVMSAIYWFVFTRIFHRDVGEDPYIVFLISGLLPWVWFNSSVSDFTKAFKRDSRLVR
                     STAIPRSIWVNRIVLSKGIEFLFSMPVLAIFVVANMIFEKNPEQVAHIGWGILWLPVA
                     ILLQTVLLVGLGLIVAPLCALYADLERTTGLILRAMFYATPIIYNVSNLPGAFETIGA
                     FNPLAGIFTLYRMAFFPDQWHTQTVIISAVMSLLILALGVWVFRALERPVLKEL"
     gene            12827..13564
                     /locus_tag="VN041_00890"
     CDS             12827..13564
                     /locus_tag="VN041_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27635.1"
                     /translation="MSAAIEVQDLGVHFRRNRRGRRSFKDLFAGASRRSRPGEFWALR
                     NVSFSVQPGESIGVVGRNGQGKSTLLRLVAGVLLPDEGGVEVHGGVAPLIELTGGFVG
                     DLTVRENVRMTAGLHGMSRAEIASRYDEIIGFAELEGFEDTPYKHLSNGMKVRLAFSV
                     VSQLDEPILLVDEVLAVGDKAFRDKCYTRIDELLADGRTLFFVSHNERDLRRFCTRGL
                     YLDKGVLAMDAPIADVLDRYNADYNMR"
     gene            complement(13574..14161)
                     /locus_tag="VN041_00895"
     CDS             complement(13574..14161)
                     /locus_tag="VN041_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27636.1"
                     /translation="MSEQQYLWAPTPDKPKPGRVWLIVVLAIAAVAIVGVLLWLFLRP
                     ETPAPHASPTASASPSASASPTPTPTPSASVEPSPTPPPTTAPPIPDPALSVFQGKVS
                     PVLNDARRGLQIVGESDPQEAAQNVGFLQEDAGRLAEVVAPSSIASRWSTGLDAYATS
                     LQALRKAYEGGTAADRELTAAKSALDALDDIAAGR"
     gene            complement(14161..15114)
                     /locus_tag="VN041_00900"
     CDS             complement(14161..15114)
                     /locus_tag="VN041_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU27637.1"
                     /translation="MTAAFDPTSAAIVIVTFNRSHLLKGLLTSIRTMDPKPGRVVIID
                     NASTDDTTALVESFRDDLGTEIVYRRLETNTGGSGGFSEGMRTAYELGAEWIWMMDDD
                     VEVLPDGLARMGRWSGRFKSIQGRRYDYDGSEFYWQYRIADRMGIPIPFAPSKFDASG
                     FKEMNSGCFEGMFIHRSIVKQIGLPDPRFFIYWDDQMYGWLASRLTTAVIVDEFVLRR
                     TREIKQWDMGIRHMNASSNAYRYYIMRNRAFIKHYYRTKGAYNPVLFGLGTTATFFKE
                     LIRLFFVERTVRGTSNLFRGLRDGGRIGRDRTWQPMPPLEA"
     gene            complement(15111..17021)
                     /locus_tag="VN041_00905"
     CDS             complement(15111..17021)
                     /locus_tag="VN041_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU27638.1"
                     /translation="MTHVLQNVVFPLDRDPDLLPLYADPETWSVIDEEPVRVSNRAHL
                     GNILGRHRARIVSGRRVSLGTYFNAFPASYWQHWTSVRQVRLTVHTSGPATILVYRSN
                     GSGIRQRIATREVSGEASTSFDLELTEYSDGGWIWFDVVADDKHAILEGAEWTTEQEP
                     ARLGKASLGITTYNKPDYCVDTLRALAASPDAMEFVDRVFLIDQGTQLVADQDGFDEV
                     AAKLGETLQVIRQGNLGGSGGFARSMHETLQRPESDFVQLLDDDVRLEPESLRRSIVF
                     ARYATTPVLVGGHMFDLLDRPKLHGWAEVVDEHPFMWRNLYQEKMPHDFGVANLRQSP
                     LLHMRMDADYNGWWMCLIPLDAIREVGLSLPAFIKWDDAEFCLRAGKAGFPTVSLPGV
                     ALWHVSWVNKDDTIDWQAYFHARNRIVAGLLHSSAPRGGRLLKHSLRVDLKHLMMMQY
                     YPVALRTRALRDVLSGPGHMRENLATAMPAARTLAAEFPETVVHRDPRTVLHSRRGRQ
                     VYKQTKQHEFDSPTGLALRWFTLRTLIAHWLRTPDPANVAQPEVEFGKGDAQWWRVPH
                     FDSALVSSADGSGKNIYTRDRAQYRRMLRDSILLHRRLKRNWAKLQAEYRDALPELVS
                     DASWEKTFEAQA"
     gene            17101..18090
                     /gene="galE"
                     /locus_tag="VN041_00910"
     CDS             17101..18090
                     /gene="galE"
                     /locus_tag="VN041_00910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWU27639.1"
                     /translation="MKALITGGAGYIGSTVASACTDAGIDVVLLDDLSTGRSAFGEGR
                     ELYEGDIADVTLLDRIVDEHPDIDLVIHCAARIVVPESVADPLGYYDVNVGRTITLIR
                     HLADRGITRVLFSSSAAVYRGDSGGGVDETGEIAPGSPYANTKWLVEQILRDASAAGE
                     IRAIALRYFNPIGADPQLRTGLQNPAPSHALGKIMTARDAGIPFTITGTDWDTRDGSG
                     LRDYIHVWDLARAHVAAVQRFDDIAKQDTPYVAMNVGTGDGVTVRELVDAFDRVTGEH
                     LEVVEGPRRPGDQAGAFALVGRAEQALGWKAERTVDEGVRDALAWAEKLKTLG"
     gene            complement(18116..19414)
                     /locus_tag="VN041_00915"
     CDS             complement(18116..19414)
                     /locus_tag="VN041_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU27640.1"
                     /translation="MTLLRPMAALLASAEFARAYTLTALAAAFASFMIERLTSTTTMV
                     TIFALLALVGAGMLVARREELSLLRFAPTSLLGFLTLAIVSVLWSGNKPHTVLGWLAL
                     LGMATIAITIGHVRDTLQTVRAIGDTLRWLLCISLGLEILAGILLDMPLTLFGIEGNL
                     ALGGPIQGIFGTRNLLGFMAVVALITFVIEWRTQSVSRVVGVLSVALGGFLALLSASP
                     TVVVLAAAVGVTTVALTIVRHAPPARRAIVQWLLGAAVVAGLVLAFARRHQIIQLLDA
                     GSDFSTRADLWNAILDWVSEKPVTGYGWFGPWTGGEYPFQYINFQLSNHHQSALNSYF
                     DVLLQLGVLGLALFLVLGGVATVRSWLVASVRRSVVYAWTPLLLVTLAVESMFESFTL
                     VGAGWLLLVLCALRAGQTRSWRQNIDDAHTGTLPTLGPQS"
     gene            complement(19411..20829)
                     /locus_tag="VN041_00920"
     CDS             complement(19411..20829)
                     /locus_tag="VN041_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU27641.1"
                     /translation="MAVYTKHPVAAPPAAPARETTGHLMVRAYAVLCLFSVFAHTAVY
                     NLLGTAGSAVVLVVLTLGALGIWVPAIARQRPTRFPWRRLPWAALGYGALALISVLWS
                     RWPAATVLTWSLMAAITATALMVATMLTWQEIVRALSTTLKLILGLSVVIELWVALVV
                     RHPILPNFAQLPPGKINPVSYWVRGNLFNGGRIQGIVGNAHELAMMCLFALLVFAVLY
                     AARVRRRGLLIGWAVLAVVLLVKGASTTVYLGIVVILAVAAAALIARAAKTPAARRGV
                     YLAFTAIAVVGVAAAVLLRDRVLGLFGKGSDLTGRVEIWQAVWERAITRPVFGNGFSS
                     PWVPWDPAFDRWILNHGITVFHAHDMWLDVFLQLGIVGVVVMLVAWLALLWRSWFFAV
                     DRPRWDLDAQRPYSALSMLPLLLTTALLFEGIAESAPIMLWGWMLLILLSFKMKSVPL
                     IGIGVGERTPVISHGPQARQVP"
     gene            20934..>22010
                     /gene="manA"
                     /locus_tag="VN041_00925"
     CDS             20934..>22010
                     /gene="manA"
                     /locus_tag="VN041_00925"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HWU27642.1"
                     /translation="MLLTITNEPRDYAWGSTTLLAGLEGRSPSGGPEAEVWFGDHPGD
                     PAEVHGAGTLDAVTGGSLPYLLKLLAAADPLSIQVHPTRAQAREGFAREAGLDPADPA
                     RNYRDDNHKPELIVALSDRFEALGGLRPVADTLRMLASFPDTVGVVALRERLGVGADA
                     EALRATIGWLLSGDAQAAVEDIIDGLGSADAEGFAEALRAVRGIATRYPGDPGVVVAL
                     LMNHIVLHPGEAIFLRAGLLHAYISGLGVEIMAASDNVLRGGLTPKHIDVDELLSIVD
                     TEPGVVPVQPAGGEVTEYDIPVADFALRRIELSGERTVEVTGPAMALVTAGEVAVAGS
                     DGATVPVRVGAAVFASADETALALR"
BASE COUNT         3593 a         7284 c         7510 g         3623 t
ORIGIN      
        1 ggatctgctg aaggggaagc ggttcgacct cgtcgtcgcc aacgatgcac gagcccttcc
       61 ggtggccttc gcggccgcgc acggcgcccc cgtctgggcg gacctgcatg agtgggcgcc
      121 cgaggagcag tcccacatcc tcagctggcg gttgctggtc gcgccgttca tgacttacct
      181 gtgtggcatc tacctgccgc gctgcgccgc ggtcagcacg gtgagcccgc tcatcgccga
      241 gctgtacgag aaggagttcg gagccgcggc ggacgtggtc cgcaacgccc gagacttcgt
      301 agagctctcg ccgagcccgc tgcagccggg gcgaatccgc ctggtgcaca gcggcgtcgc
      361 ggtgccggag cgctgcatcg agacgttgat cgacgcgatg cacgacctgg acgacaggtt
      421 cagtctcgac ttcttcctgg tcttcggtga cgatgactct taccgtcggc agctcgtggc
      481 tcgggccgcc ggcagcgatc ggatcacctt ccacgatccg gtcgcgccgc acgagctccc
      541 cgccgcgctg aacggcttcg acctcggcgt gtacctgctc cgtccgacga cgacgaacca
      601 ccggttcatg ctgccgaaca agttcttcga cttcgtgcag tcccgcctgg gcgtggtctt
      661 cggcagggcg gtcgagatcg atgcgctcat cgactcccac gagctggggt tgacggttga
      721 cgggttcgag ccggccgacc tggtggccgc tctgagcgca ctcaccgagg acgacgtgcg
      781 cgccttcaag cagcacgcgg atgccgccgc ccacgagctg agctcggacg tcgacgccga
      841 ggttcagcgc ggcatcatcc gccggtcgct gaaccggggc tgagcgcccc gtcgatcagg
      901 cgaggccggc cagagcggcc tgatgcgcga agtcgggcac ggcggcgacc agctcgtcga
      961 acgtgccgcg tccggcgacc tctccgtcgc gcatgaagaa gatctggtcc gcgtgacgga
     1021 tcgtcgacag acggtgggcc accacgatca gcgtccgctg accggcgaga ctcgtgatcg
     1081 actccgagac ggccgcctcg gtcttggtgt ccaacgcgct ggttgcctcg tccatgacga
     1141 gcaccagcgg gtcgacgtac aaggcccggg cgatacccag cctctgacgc tgcccgccgg
     1201 agagcgagat gcctcgctct ccgacttgtg aatcgatgcc cccatcgcgt ctggacacga
     1261 tgtcccagac gtgagcctgg cgcagcgcca tctccacgcg cgcggcatcc acgtcgtcat
     1321 cccaggtcag ggcgacgttc ctgccgatcg tcgcgtcgaa cagggcgaca tcctggggga
     1381 cgtagccgac ccgctcccgc caggagcggg ccatcgaggt gagcggggca tcgtccacgc
     1441 tgatcacgcc ctgagtgggt tcgagcaggc tcaggatcag atcgaccatc gtcgacttcc
     1501 cggatccgga cgctcccacg aaggcgacga acgacccgaa cgggatgtcg atcgacacgt
     1561 cacgcacggc gagtgctgct tccggcgagt atcggtagga cacgttgtcg agccgcaggg
     1621 tcttcggcgc ctcgggtacc gtgccatcgc cgccgccggg agcggtggcg atctgctgct
     1681 cggcctcagc gagttcgtcg agcaccttgc gcgggtacgg ggcggtcgac tgcatcatgg
     1741 acatgatcga ttggaaccgc gtgatggagg gtgccatgcg gaaaccggcc aggccgaaca
     1801 tcgcgactgc ggtgatggcg gcctcctgtc cgccgatcag gaaacctgca ccgccgacca
     1861 cgatgaagcc gccgatgatg cctgactcca gcacgtaacg gggcacctgg ccaaggaagt
     1921 aggtgttcgc tcgagcgcgc gagctctccg tccggctctt cgtgacgacg tccgcgacag
     1981 cgtcctcctt gttgcgaagg ctgatctcct tcagcgttga catgatctcc aggatcagtc
     2041 gcgaggtgcg gatggaggcg gccaggttga cctcgccggc gacgcgcgcg tgcttcgcga
     2101 tccacaggta gaggaccgcg cccagcagga ggatgtacac gagcgtgatc gcggcgatgg
     2161 cgggctgaac gagtgccagc gtggcgacga ccacgaaaag gctcaccgcc tcgccgagca
     2221 gggtcgcgcc gggaagcagg aacgagttga tcatcgcgtc cacgccgccg tctgtgaagc
     2281 gcagcatgtc agaggagttc ttcttcaggc gcagcgtcca cggagcgtgg atgtaggcgc
     2341 ggaagagccg gtcacccacg accacctggt atcgcgcgat gcggcgcgtg ccccaccaca
     2401 tgaccagcgt ggagaggagg cccttggcga tcatcatcgc gcagatcagc agaatcgcga
     2461 tgatgacgcc cattccgtcc agctcaccga tcaacggaag ccgcacgggc gtgcccgctg
     2521 cgagagggcc gatgaccagc gcgagcaggc ccagcgcggc ggcgtcgaag atggccagcg
     2581 acgccagcag ccacgagtac gtggcgatga agcggcgagc ctcgggaggc agcacactgg
     2641 tgacctcgcg atacaaggcg atgacagatt tcattcgtct cccactcggg ctcagcatgg
     2701 agccctgagc cggccccgga agtctatccg gctcaggctg ggtgaacctg gcgtgctagc
     2761 gttacgggcg catgccatgc gcccgagcgc gtccgtgcgc caatgcctat gcgtatcgtc
     2821 atcgtcagcc gcatcttcac gcccgaaccg gcggcggcct cgttcctgct ccgcgcgacc
     2881 gcggaggcgt tccgtgatgc cggccacgag gtcgaagtgc tcaccagcac gccgccgcct
     2941 gcggcatccg gggccgaccc gcgcgggatc cgcgtgcgcc gcgcgcgagt gaagcgcgac
     3001 cggcagggat acgtccgcgg atacttgtcg tacctgtcgt tcgacatccc ggtgttgttc
     3061 cgcctgctct tctcgcggcg agcggacctc tacctcgtgg agccgccccc cacgacgggc
     3121 gcggtcgtgc gcgccgtgtc cctcctgcgc cgcacgccgt acgtctacga cgcagccgat
     3181 ctctgggccg acgcggccgg tgtcgtcacc tcgtctccgc tggtgctcgg agccctgcgc
     3241 gcggtggagc gcttcgcgat gcgggggacc gcgcatgcgt tcgccatctc ggaggggctg
     3301 atcgaccgga tgcgccagat cgggatcgat gtgcccgcca cggccgtggg attcggcacc
     3361 gacaccgcgt cgttccggta cgccccccag gacccgccga ccgcgccgct gttcgtctat
     3421 cccggctcgt actcggagtg gcacggcgcc gacgtgttcc tggaggcgtt cgccggggtg
     3481 cgcgaggcgc acccggacgc ccggctgctg ttcatcggga acggctccga gcagccgcgg
     3541 atgcgcgccc gcatcgccga gctcggactg accggggtcg agttccgcga tcccgtcccg
     3601 ccggagcagc tcgcggatgt gctgagttcg gcgacggcgt cgctggccag tctcaagccc
     3661 ggcgccggat acgactacgc cttcacgacc aaggtgtacg gatcgctcgt ctcgggctgc
     3721 ccggtgatct tcgccggcgc ggggccgacg ggcccgttcc tgcggaccga gtcgcagcac
     3781 catccggtgg ggacggccgt ggagtgggat gctgcggccg cgcgggcggc gatgctggcg
     3841 gccgccgaag agccgctcgc tacctcgcga cgcgtcgagc tggcggtatg ggccgctggc
     3901 cgctattcgc tctccgcggt cgccgagcgc atcgtggagc gatccgagga gatcgtccgc
     3961 ggctgacggc accgaccccg cgttcaggag gatcctctac gattgtggtg tgaagattgc
     4021 tgtcgtcgcc ctcggaaaga tcgggcttcc cctcgccgtc cagttcgcct cttccggcca
     4081 tgatgtgatc ggagtcgatg tcaaccagag ggctgtcgac acgatcaacg cggcggtgga
     4141 gcccttcccc ggggaggcgc acctgcagga gaagctcgag gagctgatcc ccaccggtcg
     4201 cctgcgcgcg accaccgact actccgaggc gatccccggc gcggatgccg tggtcctcgt
     4261 cgctcccgtg ttcgtgaacg acgagacctg ggagccggac ttcaagtaca tggacgccgc
     4321 gacgaagtcg ctggccgagc acctgacccc cggcacgctc gtctcttatg agaccaccct
     4381 gcccgtcggt acgacccgca ctcgctggaa gccgatgctg gaggagggct ccggtctgag
     4441 cgagggcgtc gacttccacg tcgtctactc gcccgagcgg gtgctcaccg gtcgcgtctt
     4501 cgccgacctg cgcaagtacc ccaagctgat cggcgctctg tcggatgagg gcacccgtcg
     4561 cgcccgtgag ttctacgagg ccgtgctgca gttcgatgag cgtcccgacc tcgcgcagcc
     4621 gaacggtgtc tgggacctgg gctccaccga ggcatccgag atggccaagc tcgccgagac
     4681 cacctaccgt gacgtgaaca tcggcctggc caaccagttc ggcgtcttcg ccgcgagcca
     4741 cggcatcgac gtgtacaagg tgatcgaggc ctgcaactct cagccctaca gccacattca
     4801 ccgtccgggc atcgcggtcg gcgggcactg catcccggtg tatccgcgtc tgtacctgtc
     4861 gacggatccg gatgccgaca tcgtccgcgt ggcgcgtcag ttcaacgcct ccatgcctga
     4921 acgactggtt gcccaggccg ctggcgtgct gggcgacctg accggacgcc gggccgtcgt
     4981 gcttggcgcc gcctaccgtg gtggcgtgaa ggagaccgct ttctccggcg tcttcccgac
     5041 cgtcgcggcg ctgaagcagc gcggcgcgga ggtgttcgtg cacgacccgc tgtacaccga
     5101 cgacgagctg cgcggcctcg gcttcgagcc cttcgcgctc ggcgccgagg cggagctcgc
     5161 cgtcctgcag actgaccatg ccgactacaa gaccgtgtct cccgagcaga tccccgggat
     5221 caagctgctt gtcgacggtc gcaacgccac cgacgccgcg ctgtgggcgg gcacgccccg
     5281 gatcgtggtc ggcgccgcag cctgagaccg tgatctccga cgcgctgcgc cgtctcgcga
     5341 cgtcgatctt cgtcgcactg ccgtatcagg tggcggatcg tatccatccg tggcgtcgac
     5401 gggcgttccg cgacaccagt gtcacgacgg tggcttccga ccggtgcact ctgctgatcg
     5461 gaccggtcaa ttccgcagga cagggctatg cctgggctcg cgctgcggaa caccttcccg
     5521 gtgtcaccgc cgtgaatctg atgtacagaa gcagcagcga caggttctcc ttccctgccg
     5581 atcattccgt cgactcagcg gtcgcgtcgg gtgtacggcg atggcagaac gccgagaaag
     5641 cggcgattct gaagtcgttc actcatgtga tcgtcgagtc aggtgctcat ctgttcgaca
     5701 cgcacgcgcc tgtgctccac catctgcttc ggatgaagga cgctgatgtt gaggtcgcgc
     5761 tgctgttcca tggcagcgac atacgcagcc ctcgagcgca tgctcagacg gaggccgatt
     5821 ccccgttccg agatggccag tatgaagaca cgtcgctgct cgagtcgatc gcatcccgga
     5881 atcgtgtcct catcgaagcg gcgggactac ccgtcttcgt gtccacgccg gatctgctgc
     5941 tcgacgttcc cgaggcgacg tggcttccag tagcagtgcg tccggaactc tggagtgctg
     6001 gagcgaccca ggtcgcgctc gagcgccggc gtccgatcgt cgtgcacgcg cccagccgag
     6061 caggactgaa aggcagcacc ctgatcgaag agactgtgcg acggctgcac gatgaaggcg
     6121 tgatcgaata ccgagaggtc cggggcgtgc catcgagtga gatgccccgg gtgtatgggg
     6181 agagcgacgt cgttctcgat cagttctcgt tggggatcta cggggtcgcg gcgtgcgagg
     6241 cgatggctgc aggacgtctc gtcatcagcc acgtgtcgga cttcgtgcgc cgtgaagttc
     6301 ggaagcggac gggactcgac cttccgatcg tcgaggcgac tgcctcaaac ctcgaatcgg
     6361 tgctgcggag ggtcgctgac tccccaagcg agtatgcggc ggtgacctcc gccggcgccg
     6421 agttcgtccg cacggtgcac gacggaagcc ggtcggcgga cgcgctgcga gcttttctgc
     6481 accgatccga gacgacggtc gagtgagcgt catctcatcc ggacaaacgg gcgttgtatc
     6541 ggagcgacgg agcacggccg ccgcccgaag tggccgcacc gcaggattcc gacctgatgt
     6601 ccaggcactg cgtgcggttg cgatcctcgc tgtcgttctg aaccacctct ggcctgcccg
     6661 cgtgaccgga ggatacgtcg gagtcgacgt gttcttcgtg atctcgggat acctgatcac
     6721 ggctcatctg ctcaaggaga tctcgtcttc ggggaggatc gcactcgcgc ccttctacgc
     6781 gagacgtgcg cgtcggctgc tccccgccgc attcctcgtg ctggccgtca gcgcggcggc
     6841 gtcgatcgcg ctgctgccgt acccgcgatg ggcgcggacc ggggcagagg tgctcgcgag
     6901 cgcggcatat gtagagaact ggttcctctc cggaatgagc gtgaattatt ctgctctgaa
     6961 cgacttggcg acccctgtcc agcactattg gtcgctgtct gtggaggagc agttctatct
     7021 cctctggccg ctgttgctga tcggttcgtg gtggctgtcg agccggctgt cgtcgaatcc
     7081 gcgccgggct gccggggtca cgctcggagt gctggtcgtc gtctcgctcg ggctcagcgt
     7141 tcacgccacc gcgctcgcac cgaaccaggc gtatttcgtc acccatgctc gcgcatggga
     7201 gttcggtata ggcgggttgg tcgcacttct gcccgctgcg cggctgtcac gtcctgtcgc
     7261 cgaggtcctg tcgctgtcag gattcgcgac gatcgcgttc gccgtggtca ccttcgattc
     7321 gctgacgccc ttccccgggt ggacggcgct cttacccgtg atgggctcgg cggccgtgat
     7381 catcggggga acggggagaa cgcgcctact gcatacgccg gtcacatcaa cgcgacccgt
     7441 gcaatggctc ggtgacatct catattctct gtacttgtgg cactggccgc tgatggtgct
     7501 gacgccgttt ctgctctcgg gcacactcac gacgtggatg aaactcggtg tgctggcgac
     7561 cgcgctgatc gtctccgatc tcacccgacg tcttgtggag aagccgggac agcgctggcc
     7621 gatgctgcgt gattccaccc gtcgcacgtt tctcgccatg gtgcttgcga tgctcctcgt
     7681 cgctgccctg acggtcagca tgatcattgc agggaaggtg cggaccgacg cggacatccc
     7741 gccgaagacg atgaccatcg aggcctgcac tggacctcag gcgctcgacg ccgcttcgtc
     7801 ctgtgatgat cccttcgggc cggcgcgcga gtcggtgatg gggccagtga acgcatactt
     7861 ctatacgccg ccgggatgtg tcggcgtccc ggatctgctc cggttcggcg gtacgacggc
     7921 cacggtgcgg tgcgacttca gcgcgggccg cgagtcgccg actgaggtgt ggctggtcgg
     7981 cgactcccat gctgagcagt tcaaggggcc gatcatcgac atcgcgcgca agcagggatg
     8041 ggtgctcacg accagcttcg gcggaggatg cccggtcatc gacgctccct tcgaaggttt
     8101 cagaacacaa gcgtcgccgg ccgataagga gaggtgtcgc acttggtcga gggcagtgca
     8161 ggatgccatc gcccttgccg ctcccgatct cgtcttcact tcgtccgccg cgcgcgtgca
     8221 gatgctcgac gacggcactg gacggacgca gagcgcgcag ttcacggatg ggctcgagcg
     8281 cacgtggcgt cgttggatct cagccggaag cacggtggtg gcgctcggcg gagtgccgtt
     8341 caacggcgag gtccggtcgc cggattgtgt cgtactcaac cgcgaagctc cgcgcgcgtg
     8401 tgcggtcccg gtcgaggtgg cgaacccgcc tgacccctat cttgccgctg ccgttgagct
     8461 ggctgattcg cgcgcgatcg ctttcgatgc ctccccgtac ttctgcgatc aggagcagtg
     8521 tttcgctgtt gtaggtggca tcgccgtcta ctacgacgcc gatcacctca atctcgacta
     8581 tgcccgtcgc ctggcgccgg agctcatggc ggtcgtcgag cgagtcttgt gaggcccgcg
     8641 tcatccgacc gacgtccggg gtggaggggc gcaagcttca ggcggcgcgc cacctcaggc
     8701 gggagtctgt cgtcgaggtc ttgcactgca tccgcagccc ggtcacggtg tgtccgatcc
     8761 acccggcgta ctgcgaggcg cagtaggcgc cagccgtgac gtatccggag tagcggggcg
     8821 gctcaggtgc ggcagccaga acaacggcgg tcttcgccga cgttgcgacg gccgtaaggt
     8881 agccgcttgc cgaaccggtc acgcggaccg agatctgctt tccgactgct gacgaagtga
     8941 tcgcgaacgt cgaacctgtc gcgccgctga cggcagcacc gtttgcgagc cactgatact
     9001 ggagcgaggt ccctgcggtc catgccccgg tgcgcacagt aagcgtcgaa ccggcccgcg
     9061 cagcgccatc gatgacaggc acggcggccg tgaggcaacc gaggttggac gcagcagcat
     9121 cgctgacgat cgctgtaccg cccagagcga tcgatgaggg ggcgccgaga attcccatcg
     9181 agacgtggac gggctcgggt acgcatctgg atgtcgtgac gtagaccgga ctcttcaatt
     9241 tgccggcgag agccgcccct gccagggcat ccggaaagtc agcgccagtg gcgaggaaag
     9301 cggcgggggc ggatcctgca ggaaagtggg cgtcattgat gcgcgctgca gtggcgtatc
     9361 gatcggcgcc accaagtcgg gtggtgtcat atgagctgga aagctgacgc tcgataccgg
     9421 ccgacacagc gcccggaccc ccgacgatgg tcacggactc ggcgccgaga cgggagatca
     9481 gcctcgatgt cgtcggagtc aggctgctca gtgagccatc gacaaggatc acgggctcgg
     9541 ctgcggcacc ggccgcaccc gtcgcggcga gcgcatccgg gaacgtcctg cctgtggcga
     9601 cgaagacgtg cgacgctgcc gtgaaggtgc tctcgacgat gctcgcgccc gtgtcgtagc
     9661 gggaagcgcc ccctaggcgg ctcacgtcgg aggtgatgcg gcggaggtca ttctcgactg
     9721 cggcgctcac cgcgccaacg ccaccggcga tgaagatccg ctcgggtgct aggcggtgga
     9781 gttcctcccg gacgacctgg ggaagggcag tcggtggcgt cagcagcagc gggccgccga
     9841 ggcgtgcggc ggccgcggcc gccgagaggg cgtcaggaaa gtcctgccct gtggccacga
     9901 agacggcctt cactccggcg gtgtatcggc gcgacacctc gaccgcggtt tcgtagcggt
     9961 tcttgccctg aagacgcgtg accccagggg gaaacaggac ggtggggtcc agctcggggt
    10021 gaaatggcga agaccgcacc atgatcgacg gcagttgcac ctctgttgtc ccgcattcac
    10081 cctgaagcgc ggtctgcaga gttgacagct cggccgcatc aacgctcaat gaccagcgat
    10141 acttgatcag cgcccaggag atcgcgtatt cgcatgagta actggtgttg gagggcatcc
    10201 agccagcggg gtcgttgtcg gccttggact ggttcgccga gttggatgcg gcgacgagcg
    10261 cataggggac atcaagatca ttggcgaatg cttgtcgctg agccgtggac caggcccacg
    10321 ctccagaccg ccaggcctcg gcgagagcca ccacatgatc gatctggatc tgcgtcacgt
    10381 cggttgtcgc aaagccgtca tacggggaga cccacgtgcc gccggtaatg gtgcagggcg
    10441 gtgcgatggt gacgggcgtc gtgctctcct gcatcaacac ctcgtagcgc gtgttgcaac
    10501 cgtcagcgtc agcgtcgatc cagtgcggaa aggcatcgcg gctgtatgcg tggttcgtgt
    10561 ccactggggc gattgcgagc agccccggca ggtcgacggc cgcagttgtc gccgccagcg
    10621 cgggcgacgc ggtagtcaag gtcgacagtg ccaagaccac ggtggagacc accacgacgg
    10681 cgcgcttgaa catgacgaag tcccaccttc gattgagtgc atggcgatac gggggcatca
    10741 ccttgctgtt cacggtagtg gatggcctgg tcggactgtt cgctcagctc aggtgagttg
    10801 acgtcactgg agccgtcacg gtctctcagc ctctccccga tacgatagac gcatggatct
    10861 cctcgtcgtc gggtcgggtt tcttcggcct caccatcgct gagcgcgccg ccgaggccgg
    10921 ccgcaaggtg accgtcatcg accgccgttc gcacatcggc ggcaacgcct acagcgaggc
    10981 ggagccggag accggcatcg aggtgcaccg ctacggcgct caccttttcc acacgtccaa
    11041 cgcgacggtg tgggagtacg tcaaccggtt cacgacgttc acgaactacg tgcaccgggt
    11101 gtacacgaac cacaacagcg tggtgtatcc gatgccggtg aacctcggca cgatcaacca
    11161 gttcttccag gcgtcgtact cgcccgatga ggcgcgcgcg ctggtgaaag agcaggcggg
    11221 ggagttcgac gtcaggacgg ccgagaactt cgaggagaag ggcatcgccc tcgtcggccg
    11281 cccgctcttc gaggcattct tccgtgacta cacggccaag cagtggcaga ccgatccgca
    11341 caagctctcc ggggacatca tcagccgcct gcccgtgcgc tacacctacg acaaccgcta
    11401 cttcaacgac acgtgggagg gcctgcccac cgacggctac accgcctggc tggagcggat
    11461 ggccgaccac cccaacatcg aggtgaagct cggcgtcgac tacttcgacg agtcgcagcc
    11521 gctcaacaag cgcgccacgg tggggcagct gcctgtggtc tacacgggcc cggtcgatcg
    11581 ctacttcgac tacgcggagg gtgcgctcag ctggcgtacg ctcgacttcg agcaggaggt
    11641 tctgaacgtc ggtgacttcc agggaaccag cgtgatgaac tacccggacc tcgacgtgcc
    11701 gttcacgcgc atccacgagt tcaagcactt ccacccggag cgcaaggacg tcttcaactc
    11761 cgataagact gtgatcatgc gcgagttctc ccggttcgcg aaccgcgagg acgagccgta
    11821 ctacccggtg aacacgcccg ccgaccgcga cggcctgctc gcctaccgcg agctcgccaa
    11881 gggcgagcag gacgtgcact tcggcggacg cctgggcacc taccagtacc tcgacatgca
    11941 catggcgatc ggctcggccc tgtcgatgtg gcgcaacgag ctctccggcc aggacgactg
    12001 acatcgggat gagcagggcc acggtcggtg ccccgggcac accgcagcgg tacgcgcact
    12061 cgctgtggct gctctccgcg cgcgatctga aggtccgcta ctccacgagc gcgctcgggt
    12121 atctgtggtc ggtgctcgac ccgctggtca tgagcgcgat ctactggttc gtcttcaccc
    12181 ggatcttcca ccgcgacgtc ggtgaggacc cgtacatcgt cttcctcatc agcggcctgc
    12241 tgccgtgggt gtggttcaac tcctcggtgt cggacttcac caaggcgttc aagcgcgact
    12301 cccgcctggt gcggtcgacg gcgatcccgc ggtccatatg ggtgaatcgc atcgtgctga
    12361 gcaaggggat cgagtttctc ttctcgatgc cggtgctggc gatcttcgtc gtcgcgaaca
    12421 tgatcttcga gaagaacccc gaacaggtcg cgcacatcgg gtggggcatc ctctggctcc
    12481 ccgtcgcgat cctgctgcag acggtcctcc tcgtgggcct cggcctcatc gtcgcgccgc
    12541 tgtgcgccct gtacgccgat ctcgagcgta cgacggggct gatcctgaga gcgatgttct
    12601 acgcgacgcc gatcatctac aacgtgtcga acctgcccgg tgccttcgag acgatcggcg
    12661 cgttcaaccc gctggcgggg atcttcacgc tctaccggat ggcattcttc ccggaccagt
    12721 ggcacaccca gaccgtgatc atcagcgcgg tgatgagcct gctgatcctc gccctcggcg
    12781 tgtgggtgtt ccgtgcgctc gagcgtcccg tgctgaagga gctgtgatgt ccgccgcgat
    12841 agaggtgcag gatctgggcg tgcatttccg ccgcaaccgc cgtggacgcc gaagcttcaa
    12901 ggacctgttc gccggggcat cccgccgttc gcgtccgggg gagttctggg cgctgcggaa
    12961 cgtgagcttc tcggtgcagc cgggggagtc gatcggtgtg gtcggccgca acggccaggg
    13021 caagtccacg ctcctgcgac tggtcgcggg cgtcctgctg cccgatgagg gcggggtcga
    13081 ggtgcacggg ggagtcgcgc ccctcatcga gctcaccggc ggattcgtgg gcgacctcac
    13141 ggttcgtgag aacgtgagga tgacggcggg tctgcacggc atgtcgcgcg cggagatcgc
    13201 gagccggtac gacgagatca tcggattcgc cgagctcgag ggcttcgagg acaccccgta
    13261 caagcatctc tccaacggta tgaaggtgcg tctggcgttc tcggtggtgt cgcagctcga
    13321 cgagccgatc ctgctcgtgg acgaggtgct cgcggtcggc gacaaggctt tccgcgacaa
    13381 gtgctacacc cgcatcgacg agctgctcgc cgacggccgc accctgttct tcgtcagcca
    13441 caacgagcgc gacctgcggc gcttctgcac gcgcggcctg tacctggaca agggcgttct
    13501 ggcgatggac gcgccgatcg ccgacgtgct cgaccgctac aacgccgact acaacatgcg
    13561 ctgagagatc tcgtcaccgc ccggccgcga tgtcgtccag cgcgtcgagc gccgacttcg
    13621 cggcggtcag ctcgcgatca gccgcggtcc cgccctcgta cgccttgcgc agcgcctgca
    13681 gcgaagtggc gtaggcgtcg agcccggtgc tccagcgcga cgcgatcgag ctcggcgcga
    13741 cgacctccgc aagtctcccc gcatcctcct gcaggaagcc cacgttctgc gccgcttcct
    13801 gggggtctga ctcaccgacg atctgcaggc cgcgtcgcgc gtcgttgagc acgggcgaga
    13861 ccttgccctg gaagacggac agagcagggt ccggaatcgg aggtgcggtc gtcgggggcg
    13921 gtgtcgggga cggctctacg gacgcgctcg gcgtcggcgt cggcgtcgga ctcgcggacg
    13981 ctgatggcga ggcgctcgcc gtgggcgacg catgcggcgc gggggtttcg ggacgcagga
    14041 acagccacag cagcacgccg acgatggcga ccgccgcgat cgccagcacc acaatgagcc
    14101 acacgcgtcc gggcttgggc ttgtcgggcg tgggggccca gaggtattgc tgctcgctca
    14161 tcacgcctcc agcggcggca tcggctgcca ggtgcggtcg cggccgatcc ggccgccgtc
    14221 gcgcaggccg cggaacaggt tgctcgtgcc gcgcaccgtg cgctccacga agaagagccg
    14281 gatgagctct ttgaagaacg tggccgtggt gcccaggccg aacagcaccg ggttgtaggc
    14341 gccctttgtg cggtagtagt gcttgatgaa ggcgcggttg cgcatgatgt agtaccggta
    14401 ggcgttgctg gacgcattca tgtggcggat gcccatgtcc cactgcttga tctcgcgggt
    14461 gcgacgcagt acgaactcgt ccacgatcac ggcggtggtg aggcgcgagg ccagccagcc
    14521 gtacatctgg tcgtcccagt agatgaagaa gcgcgggtcg gggaggccga tctgcttcac
    14581 gatcgagcgg tggatgaaca tcccctcgaa gcatccgctg ttcatctcct tgaacccgga
    14641 cgcgtcgaac ttcgacgggg cgaacgggat cgggatgccc atccgatcgg cgatgcggta
    14701 ctgccagtag aactcgctgc cgtcgtagtc gtagcggcgg ccctggatgc tcttgaagcg
    14761 gccggaccag cgccccatcc gggcgaggcc gtcggggagc acctcgacgt cgtcgtccat
    14821 catccagatc cactccgcac cgagctcgta ggcggtgcgc atgccctcgc tgaatccgcc
    14881 ggagcccccg gtgttcgtct ccaggcgacg gtagacgatc tcggtgccga ggtcgtcgcg
    14941 gaacgactcg accagggcgg tggtgtcgtc ggtggatgcg ttgtctatga tgacgacgcg
    15001 acccggcttc ggatccatcg tgcggatgct ggtgagcagc cccttgagca gatgcgagcg
    15061 gttgaaggtg acgatgacga tcgccgcgga ggtgggatcg aatgcggcgg tcatgcctgc
    15121 gcctcgaacg tcttctccca ggaggcgtcc gatacgagct cggggagtgc gtcccggtat
    15181 tccgcttgga gtttcgccca attccgcttc aatcgccggt gcagcagaat cgagtctctc
    15241 aacatgcgac ggtattgggc gcgatccctg gtgtagatgt tcttcccgga accgtcggcg
    15301 gagctcacca aggcgctgtc gaagtgcggc acgcgccacc attgtgcgtc gcccttgccg
    15361 aattcgacct cgggctgagc gacattcgca ggatctggag tgcggagcca gtgagcgatg
    15421 agcgtccgca aagtgaacca acgcagcgcg agtcctgtgg ggctgtcgaa ctcgtgctgc
    15481 ttggtctgct tgtagacctg cctgccgcga cgcgagtgca gcaccgtgcg gggatcgcgg
    15541 tgcacgaccg tctcggggaa ttccgccgcc agtgtccttg ccgccggcat ggcggtcgcg
    15601 aggttctcgc gcatgtggcc ggggccggac aacacgtcgc gcagggccct tgtgcgaaga
    15661 gccaccgggt agtactgcat catcatcagg tgcttgagat cgacgcgcag actgtgcttc
    15721 agcagccggc cgccgcgcgg cgcgctcgag tgcagcaggc ccgcgacgat gcggttgcgt
    15781 gcatggaagt acgcctgcca gtcgatcgtg tcatccttgt tcacccacga cacgtgccac
    15841 agcgccacgc ctggcagcga gacggtcggg aatccggcct tgcccgcgcg caggcagaac
    15901 tcggcatcat cccacttgat gaacgccggc agcgagagcc ccacttcgcg gatcgcgtcg
    15961 agtggaatca ggcacatcca ccagccgttg tagtccgcat ccatcctcat gtgcaggagt
    16021 ggcgactgtc gcaggttggc gacgccgaaa tcgtgcggca tcttctcctg gtagaggttg
    16081 cgccacatga acggatgctc gtcgacgacc tctgcccatc cgtgcagttt gggacggtcg
    16141 agcagatcga acatgtgccc gccgacgagt acgggcgtgg tcgcgtagcg ggcgaagacg
    16201 atcgatcgcc gcagtgactc cggctccagg cgcacatcgt cgtccagtag ttgcacgaaa
    16261 tcgctctcgg gacgctgcag cgtctcgtgc atcgagcgcg cgaagccgcc ggaaccgccg
    16321 agattgccct gccggatcac ctgcagcgtc tcaccgagct tcgcggcgac ctcgtcgaag
    16381 ccgtcctggt ccgcgaccag ctgcgtgccc tgatctatca ggaagacgcg gtcgacgaac
    16441 tccatcgcgt ctggggaggc tgcgagtgcg cgcaacgtgt cgacacagta gtcgggcttg
    16501 ttgtatgtcg tgatgccgag agatgcctta ccgagacggg caggctcctg ctccgtagtc
    16561 cactcggcac cctcgaggat cgcgtgctta tcgtctgcga cgacgtcgaa ccagatccag
    16621 cctccgtcac tgtattccgt gagctcaagg tcgaacgacg tcgaggcctc gcccgagacc
    16681 tcccgcgtcg cgatgcgctg ccggatgccg ctgccgttcg agcggtacac gaggatcgtc
    16741 gccgggccgc tggtgtgaac ggtgaggcgc acctgacgca cgctcgtcca gtgctgccag
    16801 tacgacgcgg gaaacgcgtt gaagtaggtg cccagagaaa cgcgacggcc cgacacgatg
    16861 cgggcgcgat ggcggccgag gatgttgccg aggtgcgccc tgttcgagac gcgcaccggc
    16921 tcctcgtcga tgaccgacca ggtctcgggg tccgcgtaga gagggagcag atcggggtcc
    16981 cggtcgaggg ggaagacgac gttctgcagc acatgggtca cgaggtgaca gcctaccggg
    17041 cgccgccgag ggaacgctgg aggagcgggt ggcccgggag cacgggatag catggccccg
    17101 atgaaggcat tgatcaccgg cggcgccgga tacatcgggt ccactgtcgc gagcgcctgc
    17161 accgacgcgg gtattgacgt cgtgctgctc gacgatctgt ccaccggtcg ttcggccttc
    17221 ggcgagggcc gcgagctgta cgagggcgac atcgcggacg tcacgctgct cgatcggatc
    17281 gtcgacgagc atccggacat cgacctcgtg atccactgcg cggctcgcat cgtcgtgccc
    17341 gaatcggtgg ccgacccgct ggggtattac gacgtgaacg tcgggcgcac gatcaccctg
    17401 attcggcatc tggccgaccg ggggatcacg cgggtgctgt tcagctcctc ggcagcggtg
    17461 taccgcgggg actcaggtgg tggtgtcgac gagaccggcg agatcgcacc cggcagtccg
    17521 tacgccaaca ccaaatggct ggtcgagcag atcctgcgcg acgcgtcggc ggcaggggag
    17581 atccgggcga tcgccctgcg ctacttcaac ccgatcggcg cggacccgca gctgcgcacc
    17641 ggtctgcaga atccagcgcc ctcgcatgcg ctgggcaaga tcatgacggc gcgggacgcg
    17701 ggcattccgt tcacgatcac cggcaccgac tgggacacgc gcgacggctc gggcctgcgc
    17761 gactacatcc acgtctggga cctggcccgg gcgcatgtgg ccgccgtcca gcgcttcgac
    17821 gacatcgcga agcaggacac gccctatgtc gcgatgaacg tggggacggg tgacggcgtg
    17881 acggtccgcg aactcgtcga cgccttcgac cgcgtcacgg gagagcacct cgaggtcgtc
    17941 gaggggcctc gccggcccgg cgaccaagcc ggcgccttcg cgctcgtcgg acgcgcggag
    18001 caggctctcg gttggaaggc ggagcggacc gtcgacgagg gcgtccgcga tgccctggcc
    18061 tgggccgaga agctcaagac gctcggctga gccgagatcc cgggcggtga ctcggctagc
    18121 tctgcgggcc cagcgtcggc agcgtgccgg tgtgcgcgtc gtcgatgttc tgacgccacg
    18181 acctggtctg tcccgctcgc agagcacaca gcaccaggag cagccagccg gccccgacga
    18241 gcgtgaagct ctcgaacatc gactccacgg cgagcgtcac cagcagcagc ggagtccagg
    18301 cgtacacgac cgaacgccgc acgctggcca ccagccagga acgcacggtg gcgacgcctc
    18361 cgagcaccag gaacagggcg agtcccagca cccccagctg cagcagcacg tcgaagtacg
    18421 agttcagcgc cgactggtgg tggttcgaca gctggaagtt gatgtactgg aacgggtact
    18481 cgccgccggt ccagggaccg aaccagccgt aacccgtgac gggcttctca ctgacccagt
    18541 cgaggatggc gttccacagg tccgcgcggg tcgagaagtc cgagcccgca tccagcagct
    18601 ggatgatctg atgccgccgc gcgaacgcca gtacgagccc tgccaccacc gctgcaccca
    18661 gcagccactg cacgatcgcg cggcgggcgg gcggggcgtg ccgcacgatg gtgagcgcga
    18721 cggtcgtcac gcccacggcg gccgcgagca cgaccacggt cggcgaggcg gacagcagtg
    18781 cgaggaagcc gcccagcgcg acggacagca cgccgaccac gcggctcacc gactgggtgc
    18841 gccattcgat cacgaacgtg atcagcgcca ccacggccat gaacccgagc aggttgcggg
    18901 tgccgaagat gccctggatc ggaccgccca gcgcgaggtt cccctcgatt ccgaaaaggg
    18961 tgaggggcat gtccagcagg atgccggcga ggatctcgag ccccagcgag atgcacagca
    19021 gccagcgcag ggtgtcaccg atggcgcgca ccgtctgcag ggtgtcgcgc acgtgtccga
    19081 tcgtgatcgc gatggtcgcc atgcccagca gcgccagcca tccgagcacc gtgtgcggtt
    19141 tgttccccga ccacagcacg ctgacgatcg cgagggtcag gaaaccgagc agcgaagtgg
    19201 gggcgaaacg cagcagcgag agctcctcgc ggcgggcgac cagcatcccg gcgccgacca
    19261 gggcgagcag ggcgaagatc gtcaccatcg tggtcgtcga cgtcagccgc tcgatcatga
    19321 acgatgcgaa ggccgcggcg agcgcggtga gcgtgtaggc gcgggcgaac tccgccgatg
    19381 ccagcagcgc cgccatgggc cgtagcaagg tcatggcacc tgccgggcct gcggtccgtg
    19441 cgagatcacg ggcgtgcgct cgccgactcc gatgccgatc agcggcaccg acttcatctt
    19501 gaacgacagc aggatcagca gcatccagcc ccacagcatg atcggcgcgg actccgcgat
    19561 cccctcgaag agcagcgcgg tggtcagcag caggggcagc atggacagcg ccgagtacgg
    19621 tcgctgcgcg tcgaggtccc accgcggacg gtccaccgcg aagaaccacg agcgccagag
    19681 cagcgcgagc caggcgacga gcatcaccac gacgccgacg atcccgagct gcaggaagac
    19741 gtccagccac atgtcgtgcg cgtggaagac ggtgatcccg tggttcagga tccagcggtc
    19801 gaaggcggga tcccatggga cccacggcga cgagaacccg ttgccgaaca ccggtcgcgt
    19861 gatcgcccgt tcccagacgg cctgccagat ctccacccgg ccggtcaggt cggagccctt
    19921 tccgaagagc cccagcacgc ggtcgcggag cagcacggcg gcggcgacgc cgacgacggc
    19981 gatcgcggtg aacgcgaggt acacgccgcg acgtgccgcg ggagtcttcg cggcgcgggc
    20041 gatgagcgcg gcggcggcga cggcgaggat gacgacgatg ccgaggtaga cggtcgtgga
    20101 ggcgcccttc accagcagca cgacggcgag cacggcccac ccgatcagca ggccgcgtcg
    20161 gcgcacccgc gccgcgtaga gcacggcgaa gaccagcagc gcgaacaggc acatcatcgc
    20221 cagctcgtgc gcgttgccga cgatcccctg gatccgtccg ccgttgaaga ggttcccgcg
    20281 cacccagtag ctgaccgggt tgatcttgcc cggcggcagc tgggcgaagt tcgggaggat
    20341 cggatgccgc accaccagcg ccacccacag ctcgatcacc acggagaggc cgaggatgag
    20401 cttcagtgtg gtcgagagcg cccgcacgat ctcctgccag gtgagcatcg tcgccaccat
    20461 cagcgcggtg gcggtgatcg cggccatcag cgaccaggtc agcactgtcg cggcaggcca
    20521 tcgcgaccac agcaccgaga tcagcgccag cgcaccgtag cccagcgcgg cccacggcag
    20581 ccggcgccag gggaagcgtg tcgggcgctg tctcgcgatc gccggcaccc agatgcccag
    20641 cgctccgagc gtgagcacga cgagcaccac cgcggagccc gccgtcccca gcaggttgta
    20701 cacggccgtg tgcgcgaaca cgctgaacag gcacagcacg gcgtacgcgc gcaccatcag
    20761 gtgccccgtc gtctcgcggg cgggagcggc gggtggcgca gccacgggat gcttggtgta
    20821 cacggccatc gcgttcaggc taccgcccgg cggcccacgc ggcctgtgat tccgcggccc
    20881 gccgcggaga cacctccggt gacgaactcc ggtcacgccc tacgctggga ggcatgctgc
    20941 tgacgatcac gaacgagccg cgcgactacg cctggggatc gacgactctg ctggccggcc
    21001 tcgaagggcg cagcccgtcg ggcgggcccg aggctgaggt ctggttcggt gatcaccccg
    21061 gcgacccggc cgaggtccac ggcgcgggca cgctcgacgc ggtgaccggc ggatcgctgc
    21121 cctacctgct caagctgctc gcggcggccg atccgctgtc gatccaggtg catcccaccc
    21181 gtgcgcaggc gcgggaggga ttcgcccggg aggcgggcct ggaccccgcg gatcccgctc
    21241 gcaactaccg tgacgacaat cacaagccgg agctcatcgt ggccctcagc gaccgcttcg
    21301 aggctctcgg tgggctgagg cccgtcgccg acactctgcg gatgctggcg tcgttccccg
    21361 acacggttgg cgtggtcgca ctgcgcgagc gcctcggcgt gggagcggac gcggaggcgc
    21421 tgcgcgccac gatcggctgg ctgctctccg gagacgcgca ggctgccgtc gaggacatca
    21481 tcgacgggct cggttcagcg gatgcggagg gcttcgccga ggcgctgcga gccgtgcgcg
    21541 gcatcgccac acgctacccg ggggacccgg gagtcgtcgt ggcgctgctc atgaaccaca
    21601 tcgtgctgca ccccggcgag gcgatcttcc tgcgggccgg actgctgcac gcctacatct
    21661 cgggtctcgg cgtggagatc atggcggcga gcgacaacgt gctgcgcgga ggactgacgc
    21721 ccaagcacat cgacgtggac gagctgctct cgatcgtcga cacagagccc ggcgtcgtgc
    21781 ccgtgcagcc ggccggggga gaggtcaccg agtacgacat ccctgtcgcc gacttcgcac
    21841 tgcgtcgcat cgagctctcc ggcgagcgga ccgtcgaggt gacggggccc gcgatggcgc
    21901 tggtcaccgc aggcgaggtc gccgtggcgg gctccgacgg agcgaccgtc ccggtgcgcg
    21961 tcggtgccgc cgtcttcgcg agtgcagacg agaccgcgct cgccctgcgc
//
LOCUS       DATMSX010000028         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_161381,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000028 DATMSX010000000
VERSION     DATMSX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161381"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VN041_00930"
     CDS             <1..410
                     /locus_tag="VN041_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27643.1"
                     /translation="LERVQVPHPEEALKRYPHQFSGGQRQRIAIACALACDPEVLLAD
                     EPTTALDVTVQAGILSLLRELADERQLAVLLVTHDLGVMSAVADVVTVMKDGRAVEHA
                     DRATLFHDPQHDYTRQLLAALPGSSIEGGDQDA"
     gene            403..1242
                     /locus_tag="VN041_00935"
     CDS             403..1242
                     /locus_tag="VN041_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27644.1"
                     /translation="MPESVVLDAQNIVVRYPGNPPVVAVNDVSLQVHAGETVALVGES
                     GSGKSSLARAAVGIEKLAGGQARFLDGEVTPMRVRRRPKHLTGIQMVFQDPSTSLNPR
                     RRIGDQIADGIATARARGAEGSKVEEWLEKVGLPAASVSKYPHQFSGGQKQRIAIARA
                     LAARPQLLVADEPISALDASTQTSVAALMRDLVQEAGAGMLFISHDLAVVQRIADRTF
                     VMYSGRVLESGPTAQLWADPQHPYTQALLAAIPEPDGLGRIPAAPSTEERAIWADVPA
                     VQR"
     gene            1335..2312
                     /locus_tag="VN041_00940"
     CDS             1335..2312
                     /locus_tag="VN041_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU27645.1"
                     /translation="MASPTVTVIVPAKDAGEYIGTALETLTRQFADPAQLKLVAIDDG
                     SSDHTGEVMRQYAERFPHAEVLVNPAPRGLATARNQGLAHVEGEAFTFLDGDDWMQPG
                     RLPVLAAALHDLDCDFVRTDHVAVKRSDRTLVRAPFTHRGVVADPRAAILPVDDRSMV
                     DYPYAWAGMLHRRVLERGLAGFGEGLFTAEDRPWIWRLHLEAESFAVVDAPHLLYRRG
                     LSDSLSKTLDHRQLHFTRALGEVLDLVEADREAERMLPKAVFSVLALSSHHLVRARRM
                     QPALRRRLRSGIRDLLARLPRDQAEAAIARIGGPRRRVLARVLREADHA"
     gene            2309..2947
                     /locus_tag="VN041_00945"
     CDS             2309..2947
                     /locus_tag="VN041_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HWU27646.1"
                     /translation="MIQLFALHSGYGLMAAAAALDSGLLGEAEQRILVPMNTASVPET
                     AIDLAGSPRFAALRARFDRVEPLNALIDPLHPTAWKPDEQDLPMLERLFRRAWGLGGA
                     QLELFVQSPQVAPARTLMALFHDAQLGVIGDGLMTYAPIRSRLPRRITERVGHVVHPD
                     VVPGVEPLVFASTDAECVPVPPPAFGAVLQEVGEAEPCGRHRRRNRARPCRR"
BASE COUNT          435 a         1157 c         1114 g          467 t
ORIGIN      
        1 tgctcgagcg cgtgcaggtc ccccaccccg aagaggcgct gaaacggtat ccgcaccagt
       61 tctccggcgg ccagcggcag cgcatcgcga tcgcgtgcgc gctggcctgc gaccccgagg
      121 tgctgctggc cgacgagccg accaccgcgc tcgacgtgac ggtgcaggcc gggatcctct
      181 cgctgctccg cgaactggcc gacgagcgac agctggccgt gctcctggtg acccatgacc
      241 tcggcgtgat gagcgccgtc gccgacgtgg tcacggtcat gaaggacggc cgcgccgtgg
      301 agcacgccga ccgcgcgacg ctgttccacg atccgcagca cgactacacc cggcagctgc
      361 tcgcggctct gcccggatcg agcatcgaag gaggcgatca ggatgcctga gagcgttgtc
      421 ctcgacgcgc agaacatcgt cgtgcgctac ccgggcaacc cgcccgtggt ggcggtgaac
      481 gacgtctcgc tgcaggtgca cgccggtgag acggtggcgc tggtgggcga atccggcagc
      541 ggcaagtcga gcctggcccg cgcggccgtc ggcatcgaga agctcgccgg cgggcaggcc
      601 cgcttcctcg acggcgaggt caccccgatg cgggtgcgcc gtcggccgaa gcacctgacc
      661 ggtatccaga tggtgttcca ggacccgtcg acctcgctga acccgcgccg tcgcatcggc
      721 gaccagatcg ccgacggcat cgccaccgcc cgcgcccgcg gcgcggaggg gtcgaaggtc
      781 gaggagtggc tggagaaggt cgggctgccc gcagcatccg tctcgaagta cccgcaccag
      841 ttctccggcg ggcagaagca gcgcatcgcg atcgcacggg cgctcgccgc ccgtccgcag
      901 ctgctggtgg ccgacgagcc gatctccgcg ctggacgcct cgacccagac cagcgtggct
      961 gcgctcatgc gcgacctggt gcaggaggcg ggggccggga tgctgttcat ctcgcacgac
     1021 ctcgccgtcg tgcagcgcat cgccgaccgc accttcgtga tgtactccgg gcgggtgctc
     1081 gaatccggtc ccaccgcgca gctgtgggct gatccgcagc atccgtacac gcaggcgctg
     1141 ctggcggcga tccccgaacc ggacgggctg ggccggatcc cggccgcacc gtcgaccgag
     1201 gagcgggcga tctgggccga cgtgccggcc gtgcagcgct gagcgccggg tgatgtcttc
     1261 agagcgctga tcgcgtcacc cgccgttcac ccgcacgtcg gcagcgtgta agcggagccc
     1321 tgcaccatga cccggtggca tctccgaccg tgaccgtgat cgtgcccgcc aaggacgccg
     1381 gcgagtacat cggcacggca ctcgagaccc tcacccggca gttcgccgac ccggcgcagc
     1441 tcaagctcgt cgcgatcgac gacgggtcct cggatcacac cggcgaggtc atgcggcagt
     1501 acgccgaacg gttcccgcat gcggaggtgc tcgtcaatcc cgcgccccgc ggccttgcca
     1561 ccgcccgcaa ccagggactc gcgcacgtcg agggcgaggc gttcaccttt ctcgacggcg
     1621 acgactggat gcagcccggc cgcctgcccg tgctggccgc cgcgttgcac gacctggact
     1681 gcgacttcgt gcgcaccgat catgtcgcgg tcaagcgctc ggaccgcacg ctcgtgcgcg
     1741 ccccgttcac gcaccgcggg gtggtcgccg atccccgggc cgcgatcctg cccgtcgacg
     1801 accgcagcat ggtcgactac ccgtacgcgt gggccgggat gctgcaccgc cgcgtgctcg
     1861 agcgcggact ggccggcttc ggcgaaggcc tgttcaccgc cgaggaccgg ccgtggatct
     1921 ggcggctgca cctggaggcg gagtcgttcg ccgtggtcga cgcaccgcac ctgctgtacc
     1981 ggcgcgggct ctcagactcg ctgtcgaaga ccctcgatca ccggcagctg cacttcaccc
     2041 gcgcgctcgg tgaggtgctc gacctcgtgg aggccgatcg cgaggcggag cggatgctgc
     2101 ccaaggccgt cttctcggtg ctggcgctca gctcccatca cctggtgcgc gctcgccgca
     2161 tgcagcccgc gctgcgcagg cggctgcgca gcggcatccg ggacctcctg gcccggctcc
     2221 ctcgggacca ggccgaggcg gcgatcgccc gcatcggcgg gccgcgacgg cgtgtcctcg
     2281 cgcgcgtgct cagggaggcg gatcacgcat gatccagttg ttcgcgctgc acagcggcta
     2341 tggtctgatg gccgccgcag ccgctctcga ctccgggctg ctcggggagg cggagcagcg
     2401 catcctcgtg ccgatgaaca cggcatccgt gcccgagacg gccatcgatc tcgccggctc
     2461 gccgcgcttc gccgcgctgc gcgcccggtt cgatcgcgtg gagccgctga acgccctgat
     2521 cgacccgctg catccgacgg cctggaagcc ggacgagcag gacctgccga tgctggagcg
     2581 gctgttccgg cgggcgtggg gattgggcgg tgcgcagctg gagctgttcg tgcagtcgcc
     2641 gcaggtcgcc ccggcgcgca cgctgatggc gctgttccat gacgcgcagc tgggcgtgat
     2701 cggcgacggt ctgatgacct acgcgccgat ccgcagccgg ctgccgcggc ggatcaccga
     2761 gcgcgtgggc catgtcgtgc atcccgacgt cgtgcccggg gtggagcccc tcgtgttcgc
     2821 ctcgaccgat gccgagtgcg tacccgtgcc gcctccggcg ttcggcgcgg tgctgcagga
     2881 ggtgggcgag gccgagccgt gcggaaggca taggcgccgt aaccgcgcac gcccttgccg
     2941 tcgatgaccg ggtccgtgaa cggctcggcc ttttccagcc cgctgcccgc ctgcgtcttg
     3001 cccatcaggt cgttgatggt gagcgcggtc gcggcatagg catcggcgcg gccggcctgc
     3061 acaccggaca gcgcgctgac cgcatccgga aacacgacca cctgccctgc cgggatgttg
     3121 accttctgcg cgtaatcgcc ctcgatggcg cccaccacca cgccaagctt ggc
//
LOCUS       DATMSX010000029         7853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19363,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000029 DATMSX010000000
VERSION     DATMSX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19363"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2871)
                     /locus_tag="VN041_00950"
     CDS             complement(61..2871)
                     /locus_tag="VN041_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HWU27647.1"
                     /translation="MSERNARARRRALATTSAFALAAAGLVAFGSGTANAASTSTDAP
                     PPTATIGADEYYMNYVAPRAEEAFDHVADTAVATAKGAVKAPLSAHALEKAKQTDQKF
                     AQGNPKAAEQLAKTEAKAIKTGKSPKNLKKSKTTQQAKLLTILVEFDGSEDFSDLYVP
                     TEFGATTCKPGAVQGPTLHNGIPNPADAAHEDNNTMWVPDFSSEHFNKMLFTEDGITE
                     RVRTDLTGPDGKPGFDISGYTMKNMYEEMSRGAYTLTGEATPWVKVPHSEGYYGATVC
                     HKNEAGEWEAGPMQDQQGHPDNPLGPGQLPIDAVAALAAQNPDFPWADYDIEDQGDRD
                     GDGNVNEPDGVIDHVVLVHAGEDKSGGGGAEGTYAIWAHSSAVAGGATIPGTNLKLSN
                     YIVQPENSGVGVFAHEYGHDLGLPDLYDTSNAGNSDIDFWDLMSSGSHSGPIFQSMPT
                     HMGLWDKWVLGWADPKVVNPGDDTTTIKVGQTSRPAKGTEDGIKVNLPDKKIVLATPH
                     SGSEMWYSGADQDWADVRLTRSIDDVPANAQFSMWNNYVIEEDWDYGYVEVSTDGGST
                     WTEQKVYDESGALVSTDDGYSDPNGRMQDFGGKKYGLTGSSNGWRHDHVDLSAYAGQD
                     IQLRLRQATDAAFEERNWFSDDFALTSDGSTVWSDDVETGDNGWTATVASFADTTGAG
                     WHRDSGTQVKAQYYLVEWRNMDGFDKGLKYGYTTVYSDEAWKVEKVPYNAPGALIWYR
                     DAMYGTSNQVTSNLTSLPSYGAKGGLLLVDSHFDPLRRTGAEAALDPSLLKNISSRAQ
                     SSNAAFGLQPTYEFKDCLVDASYNEACTTLAPQPAVSSFTDDKGWVPGLELRDGSLYY
                     RLRDASVVVPSVGGAPYTTRIVNPDGTPATGLFGVKVGNTVLGTGNPADSGVGYGTVI
                     TVKKSMQNNTAALISVTPPAAG"
     gene            3119..4618
                     /locus_tag="VN041_00955"
     CDS             3119..4618
                     /locus_tag="VN041_00955"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWU27648.1"
                     /translation="MTRYIPHFVGGKSLEPTGGRVADVFDPSTGEVQARVPLATADEV
                     RAVIENAAAAQPGWAATNPQKRARVMMRFVELVHQNMDELAALLSSEHGKTFEDAKGD
                     IIRGLDVIEFCIGAPHMLKGEYSTEVGTGIDVYSMRQPLGVVAGITPFNFPAMIPLWK
                     AGPAIASGNAFVLKPSERDPSVPVRLAELFLEAGLPAGILNVVHGDKVAVDTILTDPC
                     IQAVGFVGSTPIAEYIYSTAAANGKRAQCFGGAKNHLIVLPDADLDQAADALIGSGFG
                     SAGERCMAISVAVPVGEETADALVAKIAERLGELKVGPGMQDGVDYGPLVSAASKARV
                     EGLIQEGVDAGATLVADGRGLVVEGCENGFYLGPTLFDHVTTDMSIYREEIFGPVLSV
                     VRAADYEEALALASDNPYGNGVGIFTRDGDAARDFAARVNVGMVGVNVPIPVPIAYFT
                     FGGWKASGFGDLNQHGADGFRFYTKTKTVTSRWPSHGVREGASFVMPTM"
     gene            4666..5853
                     /locus_tag="VN041_00960"
     CDS             4666..5853
                     /locus_tag="VN041_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWU27649.1"
                     /translation="MTMIDSTVTAEEREAILGAVRDFADSELAPHAAERDEKHIFPRE
                     TLNRAGELGLGGIYVREEFGGTGLGRVDTVAIFEELAKGDPAVAAYISIHNMVVWMID
                     TYGDDAQCGRWVPGLTAMEQFGGYCLTEPGAGSDAANIATSAVRDGDDYLLTGVKQFI
                     SGAGEAGVYVVMARTGGAGSGARGISAFLVPGDAAGLSFGAPEKKMGWHAQPTRQVIF
                     DGVRVPASAMLGEEGEGFAIAMSALNGGRLNIAACSLGGAAWALEKAVQYVHERVAFG
                     EPLSEKQSILFTIADMRTELQAARLMVRDGAEAVDAKAPDATMRCAMAKRFATDAGFE
                     IANKALQLHGGYGYLQDYGVEKVVRDLRVHQILEGTNEIMRLIVGREMLRPAGSNQMR
                     SAS"
     gene            5850..6737
                     /gene="mmsB"
                     /locus_tag="VN041_00965"
     CDS             5850..6737
                     /gene="mmsB"
                     /locus_tag="VN041_00965"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HWU27650.1"
                     /translation="MTRVAFLGLGHMGLPMAVNLVKAGHEVHGFDLVPGAIDAARAAG
                     IPVADSGADAVHDAEVVITMFPAGKHVIAAYQEELLAAAAPGTLFIESSTIAVDEART
                     AHQLAIDAGHRNVDAPVSGGVVGAEAGTLAFMVGGADEDFEAALPLLEAMGKRIVHCG
                     GPGLGQAAKVCNNMILGISQIAVAEAFVLAERLGLEHQAMYDVVSQASGQCWALTTNC
                     PVPGPVPTSPANRDYQPGFAGALMAKDLGLALQAIEQTSTDARMGRLAQEIYAAFAAG
                     EGAGRDFSGIITDIRKGDV"
     gene            6818..7594
                     /locus_tag="VN041_00970"
     CDS             6818..7594
                     /locus_tag="VN041_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWU27651.1"
                     /translation="MAEYETILVEQRGRVGWITLNRPQALNALNGQVSEEVADAASAF
                     DADESVGAIVVTGSEKAFAAGADIKEMESKTGAEMIATDHFGAWTRFAAVRTPVIAAV
                     SGYALGGGCELAMMCDIILAGDNAVFGQPEVNLGVIPGMGGTQRLIRAVGYYKAAEIV
                     LSGRMVKADEAERIGLVSRVVPASDLLDEATKLAETIASKSLPSIYAAKATLDAAMET
                     SLTDGLAVEKATFAALFDTADQKEGMAAFREKRPPHFQHR"
     gene            7600..>7853
                     /locus_tag="VN041_00975"
     CDS             7600..>7853
                     /locus_tag="VN041_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27652.1"
                     /translation="MTEVLPDDSDLTLSVSDRRELVEWTIACAERMLPLFLAERPDDS
                     RPQEALDAARAFLRGELSIEAVREKAFACHAAAREAQDPSA"
BASE COUNT         1161 a         2651 c         2757 g         1284 t
ORIGIN      
        1 ccggccccgt gagggaccgg cgcttccggt tcgggctgat cgctcagccc cggcgcggga
       61 tcagcccgcg gccggcggcg tcaccgagat gagcgccgcg gtgttgttct gcatcgactt
      121 cttcaccgtg atgacggtgc cgtagcccac gcccgagtcg gcggggttgc cggtgccgag
      181 caccgtgttg cccaccttga cgccgaacag gccggtggcc ggagtcccgt cggggttgac
      241 gatgcgcgtg gtgtacggcg caccgccgac cgagggcacg accaccgatg cgtcgcgcag
      301 gcggtagtac agcgacccgt cgcgcagctc gagtccgggg acccagccct tgtcgtcggt
      361 gaagctgctc acggcaggct gcggggcgag cgtggtgcac gcctcgttgt aggaggcgtc
      421 gaccaggcag tccttgaact cgtaggtcgg ctgcaggccg aaggcggcgt tcgaggactg
      481 ggcgcggctg gagatgttct tcagcagcga cgggtccaga gctgcctccg cgcccgtgcg
      541 gcgcagcgga tcgaagtggc tgtccaccag gagcaggccg cccttcgcgc cgtagctcgg
      601 caggctcgtc aggttcgagg tgacctggtt cgaggtgccg tacatcgcgt cgcggtacca
      661 gatcagcgca ccgggggcgt tgtaggggac cttctccacc ttccaggcct cgtcgctgta
      721 caccgtcgtg tagccgtact tcaggccctt gtcgaagccg tccatgttgc gccactcgac
      781 caggtagtac tgcgccttga cctgggtccc gctgtcgcgg tgccagccgg cgccggtggt
      841 gtcggcgaag gaggcgacgg tcgcggtcca gccgttgtcg ccggtctcga cgtcatcgct
      901 ccagacggtg ctgccgtcgc tggtgagcgc gaagtcgtcc gagaaccagt tgcgctcctc
      961 gaacgcggca tccgtcgcct gacgcagtcg cagctggatg tcctggcccg catacgccga
     1021 caggtcgacg tggtcgtgcc gccagccgtt gctggagccg gtcagaccgt acttcttgcc
     1081 cccgaagtcc tgcatgcggc cgttggggtc gctgtagccg tcatcggtgg agaccagtgc
     1141 gccggactcg tcgtagacct tctgctcggt ccaggtggag ccgccgtcgg tcgagacctc
     1201 gacgtagccg tagtcccagt cctcctcgat gacgtagttg ttccacatcg agaactgcgc
     1261 gttcgcgggc acgtcgtcga tcgagcgggt cagacggacg tccgcccagt cctggtcggc
     1321 gccggagtac cacatctcgg atccgctgtg cggggtcgcc agcacgatct tcttgtcggg
     1381 caggttgacc ttgatgccgt cctcggtgcc cttcgcgggg cgggaggtct ggccgacctt
     1441 gatcgtggtg gtgtcgtcac cggggttcac gaccttgggg tcggcccagc ccagcaccca
     1501 cttgtcccag aggcccatgt gcgtgggcat ggactggaag atggggccgg agtgcgagcc
     1561 ggagctcatc agatcccaga agtcgatgtc cgagttcccc gcgttggacg tgtcgtacag
     1621 gtcgggcagg ccgaggtcgt ggccgtactc gtgcgcgaac acgccgacac cggagttctc
     1681 cggctgcacg atgtagttcg acagcttgag gttcgtgccg gggatggtcg cgccgccggc
     1741 caccgccgag gagtgcgccc agatcgcgta cgtgccctcg gcgccgccac cgccggactt
     1801 gtcctcgccg gcgtgcacga gcaccacgtg gtcgatcacg ccgtcgggct cgttcacgtt
     1861 gccgtcgccg tcgcgatcgc cctggtcctc gatgtcgtag tcagcccagg ggaagtccgg
     1921 gttctgcgcc gcgagcgccg ccaccgcatc gatgggcagc tgcccgggac cgagcgggtt
     1981 gtccgggtgc ccctgctggt cctgcatggg cccggcctcc cactcgccgg cctcgttctt
     2041 gtggcagacc gtcgctccgt agtagccctc ggagtgcggc accttgaccc acggggtcgc
     2101 ctcgccggtg agggtgtacg ccccgcggga catctcctcg tacatgttct tcatggtgta
     2161 gccggagatg tcgaagccgg gcttgccgtc ggggccggtc agatccgtgc gcacgcgctc
     2221 ggtgatgccg tcctcggtga agagcatctt gttgaagtgc tcgctcgaga agtcgggcac
     2281 ccacatcgtg ttgttgtcct cgtgcgcggc atccgccggg ttcgggatgc cgttgtgcag
     2341 ggtcgggccc tgcaccgcgc cgggcttgca ggtcgtcgcg ccgaactcgg tcggcacgta
     2401 caggtcgctg aagtcctcgc tgccgtcgaa ctcgaccagg atcgtcagca gcttcgcctg
     2461 ctgggtggtc ttcgacttct tcaggttctt ggggctcttg ccggtcttga tggccttcgc
     2521 ctcggtcttg gccagctgct cggcggcctt cgggttgccc tgggcgaact tctggtcggt
     2581 ctgcttcgcc ttctcgagcg cgtgcgcgct gagaggcgcc ttgaccgcgc ccttcgctgt
     2641 ggcgacggcg gtgtcggcca cgtggtcgaa ggcctcctcc gcacgcggcg cgacgtagtt
     2701 catgtagtac tcgtcggcac cgatcgtggc ggtgggcggc ggcgcgtccg tgctggtcga
     2761 cgcagcgttc gccgtgccgg agccgaaggc caccaggcct gcggccgcca gcgcgaacgc
     2821 cgaagtcgtc gccagggcgc gtcgtcgcgc tcgggcattc cgttctgaca tcgaatctct
     2881 cctttcacac ggcggcgcga agggtcttcg cggcaccgcg atcggcgggc gccgggtgga
     2941 cgcccggggt ctgtctatcg tgctcagccg gttcaaagga agtggtgcga gggaaaaagt
     3001 agacattcgt ctatgcggag tgtccgatgc gccagttagt aggaagtcct agtaattgcc
     3061 ctgtgtgccg actagcatga tcactgtgcc aaagaaggtg cagaggagtg tcagcacaat
     3121 gactcgttac atcccccatt tcgtaggcgg aaagtcgctc gaacccaccg gcgggcgcgt
     3181 cgccgatgtg ttcgatccga gcaccggaga ggtgcaggcg cgtgttccgc tggccacggc
     3241 cgacgaggtg cgcgcggtca tcgagaacgc cgctgccgcg cagccggggt gggccgccac
     3301 caacccgcag aagcgcgccc gcgtgatgat gcgcttcgtc gagctcgtgc accagaacat
     3361 ggacgaactg gccgcactgc tctccagcga gcacggcaag accttcgagg acgccaaggg
     3421 cgacatcatc cgcggactcg acgtgatcga gttctgcatc ggtgccccgc acatgctgaa
     3481 gggcgagtac tccaccgagg tcggcaccgg catcgacgtg tactcgatgc gccagccgct
     3541 cggcgtggtc gcgggcatca ccccgttcaa cttccccgcc atgatcccgc tgtggaaggc
     3601 cgggcctgcg atcgccagcg gcaacgcctt cgtgctcaaa ccctcagagc gcgacccctc
     3661 cgtgccggtg cgcctcgccg agctgttcct cgaggcgggc ctgcccgcgg gcatcctcaa
     3721 cgtcgtgcac ggtgacaagg tggcggtcga cacgatcctc accgacccgt gcatccaggc
     3781 cgtcggtttc gtcggctcca cgccgatcgc cgagtacatc tactcgaccg ccgcagccaa
     3841 cggcaagcgc gcgcagtgct tcggcggggc caagaaccac ctgatcgtgc tgcccgacgc
     3901 cgatctcgac caggcggccg acgcgctgat cggctccggc ttcggctcgg ccggcgagcg
     3961 ctgcatggcc atctcggtcg ccgtcccggt gggcgaggag accgccgacg cgctggtcgc
     4021 gaagatcgcc gagcgcctgg gagagctgaa ggtcgggccc ggcatgcagg acggcgtcga
     4081 ctatggaccc ctggtctccg ccgcgtcgaa ggcgcgcgtc gagggcctca tccaggaggg
     4141 cgtcgacgcc ggcgccacgc tcgtcgccga cggtcgcggc ctggtggtcg agggctgcga
     4201 gaacgggttc tacctcggac ccaccctgtt cgatcacgtc accaccgaca tgtcgatcta
     4261 ccgcgaggag atcttcggtc cggtgctctc ggtcgtgcgc gcggcggact acgaggaggc
     4321 gctggcactg gcatccgaca acccctacgg caacggtgtc ggcatcttca cccgcgacgg
     4381 tgacgccgcc cgcgacttcg cggcccgcgt gaacgtcggc atggtcggcg tcaacgtccc
     4441 gatcccggtg ccgatcgcct acttcacctt cggcggctgg aaggcgtccg ggttcggcga
     4501 cctgaaccag cacggagccg acggcttccg cttctacacg aagaccaaga ccgtgaccag
     4561 ccgctggccg tcgcatggcg tgcgcgaggg cgccagcttc gtcatgccca cgatgtgatc
     4621 cccgccactg ccgcacacat ccccgagatc cgaggaccga gagaaatgac catgatcgac
     4681 agcaccgtca ccgccgagga gcgcgaggcg atcctcggcg ccgtgcgcga cttcgccgat
     4741 tccgagctcg cgccccacgc cgccgagcgc gacgagaagc acatcttccc ccgcgagacg
     4801 ctcaacaggg cgggcgagct gggtctgggc ggcatctacg tgcgcgagga gttcggcggc
     4861 acgggcctgg gccgcgtgga caccgtcgcg atcttcgagg agctcgccaa gggcgacccg
     4921 gcggtcgcgg cctacatctc gatccacaac atggtcgtgt ggatgatcga cacctacggc
     4981 gacgacgcgc agtgcggccg ctgggtgccg ggtctgaccg ccatggagca gttcggcggc
     5041 tactgcctga ccgagccggg tgccgggtcg gatgccgcga acatcgccac gagcgcggtg
     5101 cgcgacggcg acgactacct gctcacgggc gtgaagcagt tcatctcggg cgcgggcgag
     5161 gccggcgtct acgtggtcat ggcgcgcacc ggcggagccg gctcgggggc gcgcgggatc
     5221 agcgcgttcc tcgtccccgg agacgccgcc ggcctgagct tcggtgcgcc ggagaagaag
     5281 atgggctggc atgcccagcc cacgcgccag gtgatcttcg acggcgtgcg ggtgcccgca
     5341 tccgcgatgc tcggcgagga gggcgagggc ttcgcgatcg cgatgtcggc gctcaacggc
     5401 ggccggctca acatcgccgc ctgctcgctg ggcggtgcgg cctgggctct ggagaaggcc
     5461 gtgcagtacg tgcacgagcg cgtcgcgttc ggcgagcctc tctccgagaa gcagtccatt
     5521 ctgttcacga tcgccgacat gcgcaccgag ctgcaggccg cgcgcctgat ggtgcgcgac
     5581 ggcgccgagg ccgtcgacgc gaaggcgccg gatgccacga tgcgctgcgc catggcgaag
     5641 cgattcgcca cggatgccgg cttcgagatc gccaacaagg cccttcagct gcacggcggc
     5701 tacgggtacc ttcaggacta cggggtcgag aaggtcgtac gcgaccttcg cgtgcatcag
     5761 atcctggaag ggacgaacga gatcatgcga ctcatcgtcg gacgcgagat gctgcgcccc
     5821 gccggttcga accagatgcg gagcgcatca tgacgcgcgt cgccttcctg ggcctcggcc
     5881 acatgggcct gcccatggcc gtgaacctgg tgaaggcggg tcacgaggtg cacggcttcg
     5941 acctcgtgcc cggcgcgatc gatgccgcgc gcgccgccgg catccccgtc gcggacagcg
     6001 gagcggatgc cgtgcacgac gccgaggtcg tcatcacgat gttccccgcc gggaagcacg
     6061 tgatcgccgc ctaccaggaa gagctgctgg ccgcggccgc gccgggcacc ctgttcatcg
     6121 agtcgtccac catcgccgta gacgaggcgc gcacggcgca tcagctggcg atcgacgccg
     6181 ggcatcgcaa cgtcgacgcc ccggtctcgg gcggcgtggt cggcgcggag gccggaacgc
     6241 tggcgttcat ggtcggcgga gcggacgagg acttcgaggc ggcccttcca ctgctcgagg
     6301 ccatgggcaa gcgcatcgtg cactgcggcg ggccggggct cggccaggcc gcgaaggtgt
     6361 gcaacaacat gatcctgggc atctcgcaga tcgcggtcgc cgaggcgttc gtgctggcgg
     6421 agcgcctggg gctggagcac caggccatgt acgacgtcgt gtcccaggca tccggccagt
     6481 gctgggcgct gaccaccaac tgccccgtgc cgggccccgt gcccaccagc ccggcgaacc
     6541 gcgattacca gcccggcttc gccggcgccc tgatggcgaa ggatctgggg ctcgcgctcc
     6601 aggcgatcga gcagacgtcg acggatgcca ggatgggccg cctcgcgcag gagatctacg
     6661 ccgcgttcgc cgcgggcgag ggcgccggac gggacttctc ggggatcatc acagacatcc
     6721 gcaagggtga cgtctgaccc ctcccgttca ggaaccggaa aggttcccac ctggaatact
     6781 ggtcgtgccg cagtgctgcg gctgagggga gtgacacatg gccgagtacg agacgatcct
     6841 tgtcgaacag cgaggacggg tgggctggat caccctcaac cggccgcagg cgctcaacgc
     6901 cctgaacggt caggtgtccg aggaggtcgc ggacgcggca tccgccttcg acgcggatga
     6961 gagcgtcggc gccatcgtcg tcaccggctc cgagaaggcg ttcgccgccg gcgcggacat
     7021 caaggagatg gagtcgaaga ccggtgccga gatgatcgcc accgaccact tcggcgcgtg
     7081 gacccgcttc gccgccgtgc gcacccccgt gatcgcggcg gtctccggct acgcgctggg
     7141 cggcggatgc gagctggcga tgatgtgcga catcatcctc gcgggcgaca acgccgtgtt
     7201 cgggcagccc gaggtcaatc tcggagtgat cccgggcatg ggcggcacgc agcgtctcat
     7261 ccgcgcggtc ggctactaca aggccgccga gatcgtgctg tccggccgca tggtgaaggc
     7321 cgacgaggcg gagcgcatcg gcctcgtgtc gcgtgtggtg ccggcatccg acctgctcga
     7381 cgaggccacg aagctcgctg agaccatcgc gtcgaagtcg ctgccgtcga tctacgccgc
     7441 caaggcgacg ctggatgccg cgatggagac gtcgctcacc gacgggctcg ccgtcgagaa
     7501 ggcgacgttc gccgccctgt tcgacaccgc ggaccagaag gaggggatgg ccgccttccg
     7561 cgagaagcgc ccccctcact tccagcaccg atgagcgcca tgacagaggt gctgccggac
     7621 gactccgacc tgaccctctc ggtgtcggac cgtcgtgaac tcgtggagtg gacgatcgcc
     7681 tgcgccgagc ggatgctgcc gctcttcctc gccgagaggc cggatgactc ccgcccgcag
     7741 gaggccctgg atgcggcgcg cgcgttcctg cgcggcgagc tgagcatcga ggccgtgagg
     7801 gagaaggcgt tcgcctgcca cgcggccgcg cgtgaggcgc aggacccgtc ggc
//
LOCUS       DATMSX010000030         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180480,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000030 DATMSX010000000
VERSION     DATMSX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180480"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VN041_00980"
     CDS             complement(<1..489)
                     /locus_tag="VN041_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWU27653.1"
                     /translation="MTNRLADTLSPYLRAHAENPVDWRPWSIEAFDEAVRRDVPLLIS
                     IGYSTCHWCHVMARESFSDEATAAQINEGFVAVKIDREEHPEVDAAFMAAASAFTQNL
                     GWPLTVFTTPRGGTFYAGTYWPPEARGGTPSFRDVLTAVREAWTDRRDEVLASADAVA
                     DAH"
     gene            complement(530..1102)
                     /gene="msuE"
                     /locus_tag="VN041_00985"
     CDS             complement(530..1102)
                     /gene="msuE"
                     /locus_tag="VN041_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HWU27654.1"
                     /translation="MPAHYRVVAVSGSLHEPSKTTALLRAIADAVAERIDVDVRIIEL
                     TEIGPGLAGALRRDDLPPAVEAQLQAIEEADLLIVGSPVYRASFTGLFKHLFDFIGQY
                     ALVGKPVLLAATGGGERHALILEHQLRPLFAFFQALTLPLGVYASNTDFDGYEIRSPQ
                     ILDRIALAAERALPLVGFAPVADPRALAAF"
     gene            complement(1213..2049)
                     /locus_tag="VN041_00990"
     CDS             complement(1213..2049)
                     /locus_tag="VN041_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU27655.1"
                     /translation="MTTPTVEFNDGHSIPQLGYGVFKVPADEAERAVSQAFEAGYRHI
                     DTAAIYGNEEGVGAAIAKSGIARSELFITTKLWNDRHHDDEPDKAIAESLEKLGLEQV
                     DLYLVHWPTPAKDDYVHAWEKMIGIRERGLARSIGVSNHLVPHLERIVEATGVVPAVN
                     QIELHPAYQQREITDWAAAHGVLIESWGPLGQGKYDLFGADAVASAAAATGRTPAQVV
                     LRWHLQKGFIVFPKSVRPERMAENIDVFDFELTDAQIAAIDAMDPGDGSGRVSAHPDE
                     VN"
     gene            complement(2112..>2835)
                     /locus_tag="VN041_00995"
     CDS             complement(2112..>2835)
                     /locus_tag="VN041_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWU27656.1"
                     /translation="LAGSLHPAEDIHGTNALGGPVLPEPTVPLDERDAQTLMRDVIAA
                     SEVPVTIVATGPLTNIGLLVRDHPDLLPGIARVIWMGGSTTRGNATPYAEFNALTDPE
                     AIALVLDSGVDFTMVGLNITHQALVTREVRERIGRIGTRTAAFGWELLEFFCSTYEVT
                     QGMPDGPLHDPITVAMLIDPAVATTVRSHLDIEVNGTHTRGATSVDLIDALGAEPNAT
                     IAMDLDVDRFWALVEEAVAALA"
BASE COUNT          416 a          939 c         1043 g          437 t
ORIGIN      
        1 gtgcgcgtcg gccacggcat ccgccgaggc gagcacttcg tcgcgccgat ccgtccacgc
       61 ctcgcgcacg gccgtcagca cgtcgcggaa ggacggcgtt ccgccgcggg cctccggagg
      121 ccaataggtg ccggcgtaga acgttccgcc ccgcggcgtg gtgaacacgg tcagtggcca
      181 gccgaggttc tgcgtgaacg cggatgccgc ggccatgaac gccgcatcga cctccggatg
      241 ctcctcgcga tcgatcttca ccgccacgaa accctcgttg atctgcgcgg ccgtggcctc
      301 atcggagaag gactcgcgcg ccatgacgtg acaccagtgg caggtggagt agccgatcga
      361 gatcagcagc ggcacatcac ggcgcacggc ctcgtcgaac gcctcaatgc tccacggtcg
      421 ccagtccacc gggttctcgg cgtgcgcacg caggtagggg ctgagggtgt cggcgagccg
      481 gttggtcatg gcacccacgg tacgccgcgg gccccggacc gcgggccggt cagaatgcgg
      541 cgagcgcgcg cgggtcggcg accggcgcga atccgaccag cggcagcgcc ctctcggcgg
      601 cgagcgcgat gcggtcgagg atctgcggcg accggatctc gtagccgtcg aagtcggtgt
      661 tgctggcgta cacgcccagc ggcagggtga gcgcctggaa gaaggcgaac agcggtcgca
      721 gctggtgctc gaggatcagg gcgtgccgtt caccgccgcc ggtggcggcc agcagcacgg
      781 gcttgccgac cagggcgtac tggccgatga agtcgaacag gtgcttgaac agcccggtga
      841 acgaggcgcg atagaccggg ctccccacga tgagcaggtc ggcctcctcg atcgcctgca
      901 gctgcgcctc caccgcggga ggcaggtcgt cgcgccgcag agcgccggcg agcccaggtc
      961 cgatctcggt gagctcgatg atgcgcacgt ccacgtcgat ccgctccgcc actgcgtccg
     1021 cgatggcgcg cagcagggcg gtggtcttgc tgggctcgtg cagggatccg gagacggcga
     1081 cgacgcggta gtgggctggc atgcctccga cggtagtgag cagaacgccc cgcgtcacgg
     1141 gaagcgacac ggggcgtcat gcagtcgaaa catggcgtca tccggcgggc ggccggggac
     1201 ggcgggagga ggtcagttga cctcgtccgg gtgggcgctg acgcggcccg agccgtcgcc
     1261 cgggtccatg gcgtcgatcg cggcgatctg cgcatcggtc agctcgaagt cgaacacgtc
     1321 gatgttctcg gccatccgct cggggcgcac cgacttcggg aagacgatga agcccttctg
     1381 caggtgccag cgcagcacga cctgggcagg ggtgcgcccg gtggccgcag cagccgatgc
     1441 caccgcatcc gcgccgaaca ggtcgtactt gccctggccg agcggacccc acgactcgat
     1501 gagcacgccg tgcgcggcgg cccagtcggt gatctcgcgc tgctggtagg cggggtgcag
     1561 ctcgatctgg ttcacggccg ggacgacgcc ggtcgcctcg acgatgcgct ccaggtgcgg
     1621 gacgaggtgg ttcgagacgc cgatcgagcg ggcgaggccg cgctcgcgga tgccgatcat
     1681 cttctcccag gcgtgcacgt agtcgtcctt ggccggcgtg ggccagtgca ccaggtacag
     1741 gtcgacctgc tcgagaccca acttctcgag gctctcggcg atggccttgt cgggctcgtc
     1801 gtcgtggtgg cggtcgttcc acagcttggt ggtgatgaac agctccgagc gggcgatgcc
     1861 gctcttcgcg atggcggcgc cgacgccctc ctcgtttccg tagatggccg cggtgtcgat
     1921 gtgacggtag ccggcctcga atgcctggct gaccgcgcgc tcggcctcat cggccggcac
     1981 cttgaagacg ccgtagccga gctgggggat gctgtgtccg tcgttgaatt caaccgtggg
     2041 agtcgtcatg gctccagcgt acggggttgt gatgacaggt gcgatgacgg agtccgcccg
     2101 atgccacggc ctcaggcgag cgcggcgacc gcctcctcga cgagcgccca gaagcggtcc
     2161 acgtcgaggt ccatcgcgat ggtcgcgttg ggttccgcgc ccagtgcgtc gatgaggtcc
     2221 acgctcgtcg cgccgcgcgt gtgggtcccg ttgacctcga tgtcgaggtg cgagcgcacc
     2281 gtggtcgcca cggccggatc gatgagcatc gccacggtga tcgggtcgtg cagcggcccg
     2341 tcgggcatgc cctgcgtcac ctcgtacgtg gagcagaaga attccagcag ctcccagccg
     2401 aaggccgccg tgcgggtccc gatccgcccg atgcgctcgc gcacctcgcg ggtgacgagc
     2461 gcctgatgcg tgatgttcag cccgaccatg gtgaagtcca ccccgctgtc gagaaccagt
     2521 gcgatggcct ccgggtcggt caaggcattg aactccgcgt agggcgtcgc gtttccgcgc
     2581 gtcgtggagc cgcccatcca gatcacccgc gcgatgccgg ggagcaggtc ggggtggtcg
     2641 cgcaccagca ggccgatgtt cgtcagcggc ccggtcgcga cgatcgtgac gggcacctcg
     2701 gatgccgcga tgacgtcgcg catgagggtc tgcgcgtccc gctcgtcgag cggcacggtc
     2761 ggctcgggga ggacgggccc acccagcgcg ttggtgccgt ggatgtcctc ggcggggtgc
     2821 agggaaccgg cgagc
//
LOCUS       DATMSX010000031         9280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_76259,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000031 DATMSX010000000
VERSION     DATMSX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76259"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2119
                     /gene="gcvP"
                     /locus_tag="VN041_01000"
     CDS             <1..2119
                     /gene="gcvP"
                     /locus_tag="VN041_01000"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWU27657.1"
                     /translation="SPGSLGADVAVGTTQRFGVPMGFGGPHAGYMSVRAGLERQLPGR
                     LVGVSQDAVGYPAYRLALQTREQHIRREKATSNICTAQVLLAVMASMYAVYHGPDGLR
                     AIARDVAANAALLRDWLVEAGAEVVHDAFFDTLNVRVVGGAAALVEKAHEAGILLRLV
                     DADTAGIAVDETTTVTDLHRVAVLFGGKEERVFGRFDGGSTGALPTVLLREDEYLTHP
                     VFHAHRSETAMMRYLKQLADRDYALDRGMIPLGSCTMKLNAATEMAAITWPEFAQLHP
                     FAPADDVEGSLAMIDQLEAWLAEVTGYDAVSLQPNAGSQGELAGLLAIRGYHHANGDT
                     HRDVCLIPSSAHGTNAASAVLAGMKVVVVATDELGNVDLGDLRAKIEQHAENLAALMI
                     TYPSTHGVYEEQVVEITSAVHDAGGQVYVDGANLNALLGYARFGDLGGDVSHLNLHKT
                     FAIPHGGGGPGIGPVAAKAHLAPHLPGHPQAQKDVHAGGYVFEGGPVSAAPYGSAGVL
                     PISWAYVRMMGADGLRNATAAAVLSANYIAARLADHYPVLYTGENGRVAHECILDLRP
                     LKEATGISVDDVAKRLIDFGFHAPTMSFPVAGTLMVEPTESEDLGELERFIEAMIMIK
                     AEADAVAAGRWPADDNPLVNAPHTAASLIAGEWTHAYTREEAAYPVHALVATKYWPPV
                     RRIDQAYGDRNLVCACPPIEAFA"
     gene            2299..3963
                     /locus_tag="VN041_01005"
     CDS             2299..3963
                     /locus_tag="VN041_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU27658.1"
                     /translation="MKRNKIALASAAVLVAGVTVLAGCSSSGSNPKPSADTGNGNTDP
                     KAIITASGSEPENPLIPTNTNEVGGGKILDSIFAGLAYYDGSGKLVNDMADKIEVTAP
                     DELTVTLKKGMKFTDGSDVKADNFIKAWNYGALFSNKQLNQSWFADIEGFSAEADSEL
                     TGLKKVSDTEFTIKLTSPVAADFATRLGYSAFYPLPDVAFTDMAKFGENPIGNGPYKL
                     AGDGAWQHDVQIDLVKNPDYKGVRKLANGGLTLKFYTSYDAAYADLLGGNLDVIDSIP
                     DASIATFQSDLGDRAVNQPAAIFQSFTIGEWIEHFSGDEGKLRRAAMSMAIDRESITK
                     TIFNDTRVPATDFTSPALDGYQKSLKGGDALKYDPKEAKKLWAEADKISPWSGKFEIA
                     YNANGPHQAWVEAVANSLKNTLGIDAAGASYVDFATLRAAINAKDANGKRTFKTAARS
                     GWQADYPGVYNFLFPLYSTGASSNDGDYTNPEVDKLLAEGASADSPADSAKKYTKVQE
                     ILVKDLPAIPLWYQNSIGGFGESVHNVKFGWNSVPLFEDITKDKTK"
     gene            4056..4982
                     /locus_tag="VN041_01010"
     CDS             4056..4982
                     /locus_tag="VN041_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27659.1"
                     /translation="MFSYIVRRILQLIPVFLGSTLLIYALVFAMPGDPIAALFGDKQP
                     SQQLLDAIRAQYHLDQPFIVQYYYYMSGLVRGDMGTTFSGQSVNDVLARTLPVTGRLA
                     VLSIGIEFLLSIIVGTFSALRKGKVSDNVALIIALVFLSMPIFVMCFLAQYFLAIQLG
                     WFRPTVGGQNGWGDMWLPAIVLGLSLYATSMRLMRGSVIETMNQDWVRTAYSKGLPQY
                     RVLPVHVIRNSLIPVITNSATNFGVLLVGATVTEFIFNIPGVGNTLFRAILQHEGPTI
                     VSFVTIFVVLYVLVNLLVDLLYGLLDPRIRYV"
     gene            4975..5916
                     /locus_tag="VN041_01015"
     CDS             4975..5916
                     /locus_tag="VN041_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27660.1"
                     /translation="MSDPLSRRYVAPVTTEQLPTDAVRVSGKPSNLWRDAWYDLRRRP
                     LFWISLAFVALLLLMAAWPTLFTQVPPDNLCHLENSNKGPTEGHLLGFTFQGCDVFSR
                     IVHGASTSLSVGLLSTIIGSGIGWIMGTIAGYYGGWVDSLLSRIGDMFFSIPYILAAV
                     VVMTVFEKYRNVFTLSLAIGGFAWAATARVVRAEVMRVRQAEFITASKAVGRSKFSTL
                     MAHVVPNAIAPLIVLGTLALAGGIVAESTLAFLGVGLGTETMSWGADISRAQSTLRVA
                     PMSLIYPSIALTTAVLSFVVLGELLRDAMDPKARATR"
     gene            5913..7589
                     /locus_tag="VN041_01020"
     CDS             5913..7589
                     /locus_tag="VN041_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27661.1"
                     /translation="MNASIDQEPLVSIRDLKVAFRGQHGVREVLHGVSFDIFPGETVA
                     IVGESGSGKSTTAAAIVNLLPGTGKVTGGSIVMDGRELTKLGRREMEHLRGRDIGFVP
                     QDPMSNLNPVWSIGFQVKEAIRANGIAQGRAEVKRRAIEVLQQAGLADAERRLHQFPH
                     QFSGGMRQRALIGIGLAADPRLLIADEPTSALDVTVQRVILDHMASLTRDRGTSVLLI
                     THDLGLAAERASRIIVMNQGEIVEAGPSREILEDPRHPYTKRLVAAAPSIASQRIQAE
                     VTEHGIQTEESLADAPTMVRVAGLTKDFKIRQGGFRSELFRAVDDVSFDIPRGKTLAL
                     VGESGSGKTTVARMVLKLESVTSGTIEVAGEEVSHLSNAQSFALRRRMQPVFQDPYGS
                     MDPLRNIGNTIAEPMVVHGVGDQSSRRERVLELLEQVALPSEVVSRYPNELSGGQRQR
                     VAIARALALKPDVVVLDEAVSALDVLVQAQILKLLAELQSELQLTYLFITHDLAVVRV
                     SSDLVAVMEKGKLVEKGSVDEIFANPQQDYTQRLLEAIPGASIQLGGGGL"
     gene            7586..8161
                     /locus_tag="VN041_01025"
     CDS             7586..8161
                     /locus_tag="VN041_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWU27662.1"
                     /translation="MSSPDKETDESRPRTYRAWTGLVFLAISALVVAYLLIDAAVRAG
                     FGQMMLLAPWVLLALWVVYAASAASLLRLDERGVTMQNLLRRISFGWAHVKDVDFRWQ
                     LEFSLDDGSKATAMGGPARSRPRRQTARERDVEGVKPPSGVRELADIRRRWKAARQDA
                     DAPIRRSWDWPALGALVVIAVWAVVSIAVTR"
     gene            complement(8198..8995)
                     /locus_tag="VN041_01030"
     CDS             complement(8198..8995)
                     /locus_tag="VN041_01030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWU27663.1"
                     /translation="MPAARILPSVDPVPGRTRLWWEIGIVLALGLGQSAIYSIVQLAY
                     RLTDSTPLADQTTTLNPSRSDREAFDLIYQLLSIGFALVPALLVCFLLWRSQRPHLQA
                     LGLDGRRYGRDTGWGALLVLAIGIPGIGLYLLGRALGLFVAVDPGGLAAHWWTVPILL
                     LSAARASIQEEFVVLGYLFARLRQLGWGTWPIIVATSALRASYHLYQGPGAFIGNFAM
                     GMLFGWLFARTGRLMPFLVAHFLIDATAFVGYPWAAAMWPALFGLPG"
     gene            9037..>9280
                     /locus_tag="VN041_01035"
     CDS             9037..>9280
                     /locus_tag="VN041_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27664.1"
                     /translation="MTSTTRRAASGGTAPAPAPRSVVVLRALLATWRDYDEKSAWYQG
                     WFLPSVVADHVAAARSDVERELLTDLHAAMTEPQWRA"
BASE COUNT         1509 a         3237 c         3096 g         1438 t
ORIGIN      
        1 ctcgccgggg tcgctcggtg ccgacgtcgc cgtgggcacc acgcagcgct tcggcgtgcc
       61 gatgggcttc ggcggaccgc acgcgggcta catgtccgtg cgcgcgggcc tcgagcgtca
      121 gctgccgggt cgtctcgtcg gcgtctcgca ggatgccgtg ggctacccgg cgtaccgtct
      181 cgccctgcag acgcgtgagc agcacatccg ccgcgagaag gccacctcga acatctgcac
      241 cgctcaggtg ctgctggccg tgatggcctc gatgtacgcc gtctaccacg gccccgacgg
      301 cctgcgcgcg atcgcgcgcg acgtcgccgc gaatgccgca ctcctgcggg actggctcgt
      361 cgaggccggt gccgaggtcg tgcacgatgc gttcttcgac accctgaacg tgcgagtggt
      421 gggcggcgcg gccgcactgg tcgagaaggc gcacgaggcc ggcatcctgc tgcgcctggt
      481 ggatgccgac accgccggca tcgcggtcga cgagaccacc accgtcaccg acctgcaccg
      541 cgtcgccgtg ctgttcggcg gcaaggagga gcgcgtgttc gggcgcttcg acggcgggtc
      601 cacgggcgcg ctgcccacgg tcctgctgcg cgaggacgag tacctcacgc atccggtgtt
      661 ccacgcgcat cgctccgaga cggcaatgat gcgctacctc aagcagctcg ccgaccgcga
      721 ctacgcgctc gaccgcggca tgatcccgct gggctcgtgc acgatgaagc tcaacgcggc
      781 caccgagatg gcggccatca cctggcccga gttcgcccag ctgcacccgt tcgccccggc
      841 ggacgacgtc gagggctcgc tcgccatgat cgaccagctc gaggcgtggc tcgccgaggt
      901 caccggctac gatgcggttt cgctgcagcc gaacgccggc tcgcagggcg agctcgccgg
      961 cctgctcgcg atccgcggct accatcacgc caacggcgac acgcaccgcg acgtgtgcct
     1021 catcccgtcg tcggcccacg gcacgaacgc ggcatccgcc gtgctcgcgg gcatgaaggt
     1081 cgtcgtcgtg gcgaccgacg agctcggcaa cgtcgacctg ggcgacctgc gcgccaagat
     1141 cgagcagcac gccgagaacc tcgccgcact gatgatcacc tacccgtcca cgcacggtgt
     1201 gtacgaggag caggtcgtcg agatcacgtc cgccgtgcac gacgccggcg gccaggtgta
     1261 cgtcgacggt gccaacctca acgccctgct cggatacgcc cgcttcggcg acctgggcgg
     1321 cgacgtctcg cacctcaacc tgcacaagac gttcgccatc ccgcacggcg gcggcggacc
     1381 gggcatcggc ccggtggctg ccaaggctca cctggcgccg cacctccccg gccacccgca
     1441 ggcgcagaag gacgtgcacg cgggcggata cgtgttcgag ggcggtccgg tctcggctgc
     1501 accgtacggc tcggcgggcg tgctgcccat ctcctgggcg tacgtgcgca tgatgggcgc
     1561 ggacggcctg cgcaacgcca cggctgcggc cgtgctgtcg gcgaactaca tcgccgcgcg
     1621 cctggccgac cactacccgg tgctctacac cggtgagaac ggccgcgtcg cccacgagtg
     1681 catcctggac ctgcgtccgc tcaaggaggc caccggcatc agcgtcgacg acgtcgccaa
     1741 gcgtctcatc gacttcggct tccacgcgcc gaccatgtcg ttccccgtcg cgggcacgct
     1801 catggtcgag ccgacggagt cggaggacct gggcgagctg gagcggttca tcgaggcgat
     1861 gatcatgatc aaggccgagg cggatgccgt ggcggcgggt cgctggccgg cggacgacaa
     1921 cccgctggtg aacgcacccc acaccgccgc gtcgctcatc gcgggggagt ggacgcacgc
     1981 gtacacgcgc gaggaggcgg cgtaccccgt gcacgcgctg gtggcgacga agtactggcc
     2041 gccggtgcgc cgcatcgacc aggcgtacgg agaccgcaac ctggtgtgcg cctgcccgcc
     2101 gatcgaggcg ttcgcctgag agcgctccct cgccatcacc ccgtctccgg ttccggcgac
     2161 ggggtgattc ggttaagacg tcataacgct tgtctcacgg agcgggtcct tcgcgggagt
     2221 ccgtcttagg atcggttcat gcgccgccct cgcagctgag gatggcgtca cgctctgaag
     2281 tccataggag gacacaaagt gaagcgcaac aagatcgcac tcgccagtgc ggcagttctg
     2341 gtcgcgggcg tcaccgtgct cgcaggctgc tcgtcgtccg gctccaaccc gaagccgagc
     2401 gccgacacgg ggaacggcaa caccgatccc aaggccatca tcacggcaag cggttccgag
     2461 cccgagaacc cgctgatccc gaccaacacc aacgaggtcg gcggtggcaa gatcctcgac
     2521 tcgatcttcg cgggtctggc ctactacgac ggcagcggca agctcgtcaa cgacatggcc
     2581 gacaagatcg aggtcaccgc acccgacgaa ctgaccgtga ccctgaagaa gggcatgaag
     2641 ttcaccgacg gcagcgatgt caaggccgac aacttcatca aggcctggaa ctacggagcg
     2701 ctgttctcca acaagcagct gaaccagtcg tggttcgcgg acatcgaggg cttcagcgct
     2761 gaggcggact ccgagctcac cggtctgaag aaggtcagcg acaccgagtt caccatcaag
     2821 ctgaccagcc cggtggccgc cgacttcgcg acccgcctcg gctactccgc gttctacccg
     2881 cttcccgatg tcgcgttcac cgacatggcg aagttcggcg agaacccgat cggcaacggt
     2941 ccctacaagc tggccggcga cggtgcctgg cagcacgacg tgcagatcga cctggtcaag
     3001 aaccccgact acaagggcgt gcgcaagctt gccaacggcg gtctgaccct gaagttctac
     3061 acctcgtacg acgcggccta cgccgacctg ctgggtggca acctcgacgt catcgacagc
     3121 atcccggacg cctcgatcgc gacgttccag agcgacctcg gcgaccgcgc ggtcaaccag
     3181 ccggccgcga tcttccagtc gttcacgatc ggcgagtgga tcgagcactt ctccggcgac
     3241 gagggcaagc tgcgccgcgc ggcgatgtcg atggcgatcg accgcgagtc gatcaccaag
     3301 acgatcttca acgacacccg cgtccccgcg accgacttca cctcgcccgc actcgatggc
     3361 taccagaaga gcctcaaggg tggcgacgcg ctgaagtacg acccgaagga ggcgaagaag
     3421 ctgtgggccg aggccgacaa gatctcgccg tggtcgggca agttcgagat cgcctacaac
     3481 gccaacggcc cgcaccaggc ctgggtcgag gccgtcgcca actcgctgaa gaacacgctc
     3541 ggcatcgatg cggccggcgc ctcgtacgtc gacttcgcca cgctgcgcgc ggcgatcaac
     3601 gccaaggacg cgaacggcaa gcgcaccttc aagaccgcgg ctcgttcggg ctggcaggcc
     3661 gactacccgg gtgtgtacaa cttcctgttc ccgctctact cgacgggcgc ctcgtccaac
     3721 gacggcgact acaccaaccc cgaggtcgac aagctcctcg cagagggtgc atccgccgac
     3781 agcccggcgg actcggccaa gaagtacacc aaggtgcagg agatcctcgt gaaggacctc
     3841 ccggcgatcc cgctgtggta ccagaactcc atcggtggtt tcggtgagag cgtgcacaac
     3901 gtgaagttcg gctggaactc cgtgccgctg ttcgaagaca tcaccaagga caagacgaag
     3961 taaggctccg cagggctgac cgtgaggcgg cgacgtgaac gtcgtcgcct cacggcgtgc
     4021 ccacggagtg catcgtccgc acgaatcgag agtggatgtt ctcttacatc gtccgccgta
     4081 tcctgcagct gatccccgtc ttcctgggat ccacgctgct gatctacgcc ctggtgttcg
     4141 ccatgccggg cgaccccatc gccgcgctgt tcggcgacaa gcagcccagc cagcagctgc
     4201 tggacgcgat ccgcgcccag taccacctgg accagccctt catcgtgcag tactactact
     4261 acatgtcggg gctcgtccgc ggtgacatgg gcaccacctt ctccggccag tccgtcaacg
     4321 acgtgctggc ccgcactctg ccggtgaccg gccgcctggc ggtgctgtcg atcggcatcg
     4381 agttcctgct gtcgatcatc gtcggcacgt tctcggcgct gcgcaaggga aaggtctccg
     4441 acaacgtcgc gctgatcatc gcgctggtct tcctgtcgat gcccatcttc gtgatgtgct
     4501 tcctggccca gtacttcctg gcgatccagc tgggctggtt ccggccgacg gtcggcgggc
     4561 agaacggctg gggcgacatg tggctgcctg ccatcgtgct gggcctcagc ctgtacgcca
     4621 ccagcatgcg gctcatgcgc ggttccgtca tcgagaccat gaaccaggac tgggtgcgca
     4681 cggcctacag caagggcctg ccgcagtacc gcgtcctccc cgtgcacgtg atccgcaact
     4741 cgctgatccc ggtgatcacg aactccgcga ccaacttcgg cgtgctgctg gtcggcgcga
     4801 ccgtcaccga gttcatcttc aacatccccg gcgtcggaaa caccctgttc cgcgccatcc
     4861 tgcagcatga gggcccgacc atcgtctcgt tcgtcacgat cttcgtcgtg ctgtacgtgc
     4921 tggtgaacct gctcgtcgac ctgctgtacg ggctgctcga tccgaggatc cgctatgtct
     4981 gacccgctgt cccggcgcta tgtcgccccc gtcaccacgg agcagctgcc caccgacgcc
     5041 gtgcgcgtca gcggcaagcc ctccaacctg tggcgcgatg cctggtacga cctgcgacgt
     5101 cgcccgctgt tctggatctc gctggcgttc gtggctctgc tgctgctgat ggcggcgtgg
     5161 ccgacgctgt tcacgcaggt gcctccggac aacctctgcc acctggagaa ctccaacaag
     5221 gggccgactg aagggcatct cctgggcttc accttccagg gctgcgatgt gttctcccgc
     5281 atcgtgcacg gcgcgagcac ctcgctgagc gtgggcctgc tgtccacgat catcggctcg
     5341 ggcatcggat ggatcatggg cacgatcgcc ggctactacg gtggctgggt cgactcgctg
     5401 ctctcgcgca tcggtgacat gttcttctcg atcccctaca tcctggccgc cgtggtcgtg
     5461 atgacggtgt tcgagaagta ccgcaacgtc ttcacgctgt cgctcgcgat cggcgggttc
     5521 gcgtgggcgg ccaccgcgcg cgtggtccgc gccgaggtga tgcgcgtgcg gcaggcggag
     5581 ttcatcacgg cgtccaaggc cgtcgggcgc tcgaagttca gcaccctgat ggctcacgtc
     5641 gtgcccaacg caatcgcgcc gctgatcgtg ctcggcacgc tcgccctggc gggcggcatc
     5701 gtggcggagt cgaccctggc gttcctcggt gtcggcctcg gcacggagac gatgtcctgg
     5761 ggtgccgaca tcagccgcgc tcaatcgacg ctgcgggtcg ccccgatgtc actgatctac
     5821 ccgtcgatcg cactgacgac ggccgtgctg tccttcgtcg tgctgggcga gctcctccga
     5881 gacgccatgg acccgaaggc gagggccacc cgatgaacgc ttcgatcgat caggagccgc
     5941 tggtcagcat ccgcgacctg aaggtcgcct tccgcggcca gcacggcgtg cgcgaggtgc
     6001 tgcacggcgt gagcttcgac atcttccccg gcgagaccgt ggccatcgtg ggtgagtcgg
     6061 ggtccggcaa gtccacgacc gcggcggcca tcgtcaacct gcttcccggt accggcaagg
     6121 tgacgggcgg gtcgatcgtc atggacggac gcgagctgac caagctcggc cggcgtgaga
     6181 tggagcacct gcgcggccgg gacatcggct tcgtcccgca ggacccgatg tcgaacctca
     6241 acccggtgtg gagcatcggc ttccaggtca aggaggcgat ccgcgccaac ggcatcgccc
     6301 agggcagggc cgaggtcaag cggcgcgcca tcgaggtgct gcagcaggcg ggtctggcgg
     6361 atgccgagag gcgcctgcac cagttccccc accagttctc gggaggcatg cgccagcgcg
     6421 cgctgatcgg tatcggcctt gcggccgatc cgcgcctgct catcgccgac gagcccacct
     6481 ccgcgctgga cgtcaccgtg cagcgcgtga tcctcgacca catggcgagc ctcacgcgcg
     6541 accgcggcac ctcggtgctc ctcatcacgc acgacctggg tctggccgcc gagcgcgcct
     6601 cccggatcat cgtgatgaac cagggcgaga tcgtcgaggc cggtcccagc cgcgagatcc
     6661 tcgaggatcc gcggcatccg tacaccaagc gcctggtcgc tgccgctccg agcatcgcct
     6721 cgcagcggat ccaggccgag gtgaccgagc acggcatcca gaccgaggag tcgctcgccg
     6781 acgcacccac catggtgcgc gtggcgggac tgacgaagga cttcaagatc cgacagggcg
     6841 gtttccgcag cgagctgttc cgcgctgtcg acgacgtcag cttcgacatc ccccggggta
     6901 agacgctggc gctcgtgggc gagtccggtt cgggcaagac cacggtcgcg cggatggtgc
     6961 tcaagctcga gtccgtcacc tccggcacga tcgaggtcgc gggcgaggag gtgtcgcacc
     7021 tgtcgaacgc gcagtcgttc gcgctgcgcc ggcggatgca gccggtgttc caggacccgt
     7081 acggatcgat ggatccgctg cgcaacatcg gcaacaccat cgcagagccg atggtcgtgc
     7141 acggcgtggg cgaccagtcc tcgcgccgcg agcgcgtgct cgaactcctg gagcaggtcg
     7201 cactgccgag cgaggtcgtc tcgcgctacc cgaacgagct gtccggcggc cagcgtcagc
     7261 gcgtggcgat cgcgcgcgct ctggcgctga agcccgacgt ggtcgtgctc gacgaggccg
     7321 tctcggcgct cgatgtgctc gtgcaggcgc agatcctgaa gctgctcgcc gagctgcaga
     7381 gcgaactgca gctgacctat ctgttcatca cgcacgacct cgcggtggtg cgtgtctcca
     7441 gcgacctggt ggccgtgatg gagaagggca agctggtcga gaagggcagc gtcgacgaga
     7501 tcttcgcgaa cccgcagcag gactacaccc agcgtctgct cgaagccatc cccggagcct
     7561 ccatccagct cggcgggggc ggcctgtgag ctctcctgac aaggagaccg acgagtcccg
     7621 tccgcgcacc taccgtgcgt ggacgggact cgtcttcctc gcgatatcgg cgctcgtggt
     7681 cgcgtacctg ctcatcgacg ccgccgtgcg cgccgggttc ggccagatga tgctgctcgc
     7741 cccctgggtg ctgctggcgc tctgggtcgt ctacgcggcg tccgcggcct cgctgctgcg
     7801 cctggacgag cgcggcgtga ccatgcagaa cctgctgcgc cggatcagct tcggctgggc
     7861 gcacgtgaag gacgtcgact tccgctggca gctggagttc tcgctcgacg acggatcgaa
     7921 ggccaccgcg atgggcggtc ctgcgcgttc ccgtcctcgc cggcagactg cccgcgagcg
     7981 ggacgtggaa ggcgtgaagc cgccgtccgg cgtgcgcgag ctcgccgaca tccgcaggcg
     8041 ctggaaggcc gcgcgacagg atgccgatgc tccgatccgc cgcagctggg actggccggc
     8101 tctcggcgcc cttgtggtga tcgcggtgtg ggccgtggtc tcgatcgccg tcacccgctg
     8161 acccgacggg acgtcacccg ctgaccccga cgggccgcta gccgggcagc ccgaacaggg
     8221 ccggccacat cgcggccgcc cacggatagc cgacgaacgc cgtggcgtcg atgaggaagt
     8281 gggcgaccag gaacggcatg aggcgtccgg tgcgggcgaa gagccagccg aacagcatcc
     8341 ccatcgcgaa gttgccgatg aacgcgcccg gaccctggta caggtggtag ctggcgcgga
     8401 gcgcgctggt ggcgacgatg atcggccagg ttccccagcc cagctgccgc agccgggcga
     8461 acaggtagcc gagcaccacg aactcctcct ggatcgacgc ccgagcggcc gagagcagca
     8521 ggatcggcac cgtccaccag tgcgcggcga gcccaccggg gtcgaccgcg acgaacagtc
     8581 cgagcgcccg gcccagcaga tacaggccga tgccggggat gccgatggcc agcaccagca
     8641 gggcacccca gccggtgtca cgcccgtagc gccggccgtc caggcccagc gcctgcagat
     8701 gcgggcgctg ggaacgccag agcaggaagc agaccaggag ggcgggcacg agggcgaacc
     8761 cgatcgagag cagctggtag atgaggtcga aggcctccct gtcgctgcgc gacgggttca
     8821 gcgtggtcgt ctggtcggcc aggggagtgc tgtcggtgag ccggtaagcc agctgcacga
     8881 tcgagtagat cgccgactgc ccgaggccga gcgcgagcac gatgccgatc tcccaccaca
     8941 gtcgcgtgcg gccggggacg gggtcgacgg acggcaggat ccgggcagcg ggcataggca
     9001 gatgctaccc agtgcgtcag taggcttgac cgaggaatga cgagcacgac acgacgcgcc
     9061 gcgagcggcg gcacagcacc cgctcccgcg ccgaggtcgg tggtggtgct tcgcgccctg
     9121 ctcgcgacgt ggcgagacta cgacgagaag tcggcgtggt accaggggtg gttcctgccc
     9181 tccgtggtcg ccgaccacgt cgccgccgcg cgttcggacg tcgagcgcga gctgctgact
     9241 gacctgcacg ccgccatgac cgagccgcag tggcgcgcgc
//
LOCUS       DATMSX010000032        15747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_142553,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000032 DATMSX010000000
VERSION     DATMSX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142553"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VN041_01040"
     CDS             <1..796
                     /locus_tag="VN041_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HWU27665.1"
                     /translation="FVSVLAGDTAAQTRALAHRAVHEHPDGVVVVGGDGTLTGIIDVL
                     ADAYMPVTLVPAGTGNDLARALGIPTGDPAAAASAAVEGSPRRIDLGSIASQGESRAF
                     LTIAALGFDAKVSERTNTLRHPRGPLRYYLALVIELLRLRPTDFRVRVDGGEEQSIPG
                     TLLAVGNTPSYGGGMPICVGALPDDGALDLVHVAPLGRLRLLTVFPLLLRGSHLSRPE
                     VQHRRATRVQVSAPDLVVYADGERAGAGECVIGIRPGAVTMMVPRR"
     gene            818..2452
                     /locus_tag="VN041_01045"
     CDS             818..2452
                     /locus_tag="VN041_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWU27666.1"
                     /translation="MSYDEDVVIVGSGFGGSVAALRLSEKGYRVRVLESGRRFEDEDF
                     ARTSWDVRRYLWAPRLGCFGVQRIHRLPHVMILAGAGVGGGSLNYANTLYEPGAAFFA
                     DRQWPQGTSWQTELAPHYATAKRMLGVVEHYPHTGPVERIMAGAAEDLGVGDTFRRAP
                     VGVFLGMPGVTVPDPFFDGEGPARTGCTLCGNCMVGCRVGAKNTLRKNYLHLAERRGA
                     VIEPLRTVVEVREDPRGGYLVTSERSGAWARRQRRTIRAEQVVLAAGTWGTQQLLHRM
                     KASGALPRISDAVGRLTRTNSEALDGAISTRVPESVAYTEGVAITTSFHVDDHTHVEN
                     VRYGAGSNLMGGLATLLVHGDRSLFGRLGSMAAQVLRHPVRQFRLANLHRWSERGIIA
                     LVMQTADNSLTLSLRRRLGRDVLTSRQGHGEPNPSYLPQAHRAAEAIAARMQDETGVR
                     TEARGSWPEAFGIPLTAHFLGGAVIGATPEDGVIDAWNRVWGHPGVHVVDGAAVPANP
                     GVNPSLTITAIAERAFAHWPHRGGQDQRPAQDEALS"
     gene            complement(2445..3140)
                     /locus_tag="VN041_01050"
     CDS             complement(2445..3140)
                     /locus_tag="VN041_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWU27667.1"
                     /translation="MEMLLTMVSGKGVASLLKTGTAILTIVLVFPALMKLFVVTVDEG
                     WAAIRTRNGKPIIRNRPPRRKSSLSGAVGEVVVLRPGSHGAFPLFYWYRLVDVRCRAT
                     DLPVRELTGAGGVQHRVHASFEWRPIPTGHDLRVFELDVVNVRERASNIVGAALRDAV
                     RMLDGEELPHNDEISTRVLAACAESVRVACGVELVRVMITGDSLTDGYLLAGALRAGD
                     SAPDAVAALHALN"
     gene            complement(3284..5428)
                     /locus_tag="VN041_01055"
     CDS             complement(3284..5428)
                     /locus_tag="VN041_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWU27668.1"
                     /translation="MTNYDDIDYSPIMALTEGEVVTHSPVRDIRLASGKVLALITLDN
                     GRDHTRPNTLGPATLTELGETLDGLKARAAAGEIQAVGITGKQYILAAGADLSDISRV
                     GSKDNARLIAQLGHKVIGSLSDLGVPSFAFVNGLALGGGMEIALNSTYRTVDASAAAL
                     ALPEVFLGIIPGWGGAYLVPNLIGIENALEVVISNPLKQNRMLKPQQAFELGLFDAIY
                     PAANFLENSLAWADAVLGGKKVERKNAPGKIERTVKWPVAIKMARTMLESKIGTVPKS
                     PYAALELLDKARSGTKAEGFAREDEALAELVTGDQFAASMYAFDLVQKRAKRPVGAPD
                     KTLAKKVTKVGIIGAGLMASQFALLFVRKLQVPVLITDLDQARVDKGVAYIHEEIGKL
                     EAKGRLDGDTANKLRSLVTGTTDKSLYADCDFVIEAVFEEVGVKQAVFGEIEKIVAED
                     AILATNTSSLSVEEIGSKLAHPERLVGFHFFNPVAVMPLIEIVKTDATTDAALSTAFV
                     VAKGLSKNAVLTADAPGFVVNRLLAKVMGEAARAVYEGTPVADVEKAFGPLGLPMGPF
                     QLIDLVGWKVAAHVQDTMVRAFPERFYANDNFHALAELDAVVEKDKGGRVTGWTKAAE
                     KALKGQTGSTPASAETILRRVQDGLAQEIRIMLDEGVVPEVEDIDLCLILGAGWPFID
                     GGASPYLDREGASERVFQGTFHNPPIRGIAHS"
     gene            complement(5437..6642)
                     /locus_tag="VN041_01060"
     CDS             complement(5437..6642)
                     /locus_tag="VN041_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWU27669.1"
                     /translation="MAEISDVFFVDGVRTPFGRAGEKGMYWNTRADDLAVKATIGLME
                     RNASVPADRIDDVAIAATSQTGDQGLTLGRSVAILAGLPQTVPGLAVERMCAGAMTSV
                     TTMAASIGIGMYDFALAGGVEHMGHHPIGGNADPNPRFVAERMVDPGALNMGVTAERI
                     FDRFPHLTKERSDRFGMLSQHKVQAAYDAGKIQPDLVSVAIKDADGAWGLATEDEGRR
                     PQTTMEDLAALKTPFRPHGRVTAGTSSPLTDGATMSLLAGGGAVKEFGLTPKMRMVSF
                     AFAGVQPEIMGIGPIPSTEKALKKAGLTIDDIGLFELNEAFAIQVISLLDHFGIADDD
                     PRVNQWGGAIALGHPLAASGVRLMIQLAAQFAERPDVRYGLTAMCVGLGQGGSVIWEN
                     PYYDGKKRK"
     gene            complement(6716..7909)
                     /locus_tag="VN041_01065"
     CDS             complement(6716..7909)
                     /locus_tag="VN041_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HWU27670.1"
                     /translation="MAEYSVIGDRTEFEKACLALSQGQGPVAVDVERASGFRYSQRAY
                     LVQVFRRDAGVFLFDPPAVGDFSALQDAIGDTEWVFHAASQDLPSLRELGLNPPRIFD
                     TELASRLLGSDGFGLAAVVEHALGITLAKAHSAADWSTRPLPQPWLEYAALDVLHLID
                     VADVLARELAEAGKTEIAAEEFQATLDKEPKAPRTEPWRRLSGLHKVRGARQLAVARE
                     LWTAREAYAQEIDVSPGRLVPDRSLLAAVLAAPQSKSALAALKEFNGRASRTQLDRWW
                     DAIERGRTTEDLPRERVPSDALPPPRAWADRNPEADLRLKAARPVVEAIAEELHMPTE
                     NLLTPDFLRRVAWQPPALTAEAVGEALAALGARRWQVAHTAQKIADAFVEGPQTDSDA
                     PETAS"
     gene            complement(7902..8483)
                     /locus_tag="VN041_01070"
     CDS             complement(7902..8483)
                     /locus_tag="VN041_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HWU27671.1"
                     /translation="MTAHPDAAALFAVAAAELRETTFRADIVVREIPSPAGLAPFSLA
                     LAADVRPDDHGDSIYGTGRFVLLHDPDEPAAWGGAWRVIAFAQAPLEPEIGTDPLLAD
                     VAWSWLVDALDSRDAQYHSESGTSTKMLSKGFGGLAEEGDGAQIELRASWTPDAPLRP
                     HVEAWAELVGMLAGLPPGSEDVAVFGARRDGRG"
     gene            8509..9693
                     /locus_tag="VN041_01075"
     CDS             8509..9693
                     /locus_tag="VN041_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU27672.1"
                     /translation="MKSLRHAVAIAVPALLALGAAAGAVAGAAVFTVARRVVTPTVRR
                     NDTHILAVDTGAQTIELDRTPDTELPGRYGLFTSGTYGYVKLGAVLGADSRTVRRKLL
                     TQIEPGAQVDRDAAFSGWYYVTPSELHLPWESVLIGSSAGPCPAWFFPAADTGNADTW
                     VIQVHGRGTTRSECLRAVPVFHALGLPNLVVSYRNDGEAPRSRGGSYALGASEWRDVD
                     AAIGYALRHGARRVVLMGWSMGGAIALQTAVSSGNRDRIAGLVLDSPVVDWRTVLRFQ
                     AREEGLREPLPALAMGVLENTFTARLSGAENAIRFDRLDMVARASELHAPILILHSDD
                     DGFVPADASHALAAARPDLVTMPAFAVARHTKLWNYDQTRWTAAITDWMSEHGLGSRA
                     GS"
     gene            complement(9700..10131)
                     /locus_tag="VN041_01080"
     CDS             complement(9700..10131)
                     /locus_tag="VN041_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HWU27673.1"
                     /translation="MTDTHLPARLAEVREEFLELPQEDRLELLLEYSSELPAVPEHLA
                     GNVDIYERVAECQSPVFINVEVTDGVVAMHATAPPEAPTTRGFASILVQGLTGLTADE
                     VLAIPGDYPQTIGLTKSVSPLRIAGMTGMLVRAQRQVAEKR"
     gene            complement(10150..11046)
                     /locus_tag="VN041_01085"
     CDS             complement(10150..11046)
                     /locus_tag="VN041_01085"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWU27674.1"
                     /translation="MTVEHDNSSAKFAEYAAPGRLVTTEWLAANLGAPGLVVVESDED
                     VLLYETGHIPGAVKVDWHTELNDPVVRDYVDGKGFAELLSRKGISRDDTVVIYGDKNN
                     WWAAYALWVFSLFGHEDVRLVDGGRDKWIAEGREITTEKTVRPATEYPVVERDDSVLR
                     AYKDDVLAFIGQGPLIDVRSPEEYSGERTHMPAYPDESSLRGGHIPTAKSVPWSRAVA
                     EDGGFKTRAELEEIYQDGAGLQAGDDVIAYCRIGERSSHTWFVLQHLLGFENVRNYDG
                     SWTEWGSAVRVPIVTGTEPGSL"
     gene            11283..12338
                     /gene="zapE"
                     /locus_tag="VN041_01090"
     CDS             11283..12338
                     /gene="zapE"
                     /locus_tag="VN041_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWU27675.1"
                     /translation="MTQQTSTGVVHLTERTPIVTGPEMLASLVPPPQFDSATFDSYRA
                     DDAYPSQEAAKETLQRFAGRSEPVKKVGLFRRAPKEAPMKPGVYLDGGFGVGKTHLLA
                     SVYHAMPSRRKYFGSFIEYTALVGALGYRNTVDLLRGSDLLCIDEFELDDPGDTMVMT
                     RLLGELVASGTKLAATSNTPPNALGEGRFAAQDFLREIHAMSESFETLRIDGVDYRQR
                     AIDGSAVVLDDDGYAKAIAGAAAAGTASDDAFADVIRHLAQVHPSRYIRLIDGLDLVG
                     LRDVQVLTDQSEALRFVAFVDRVYDAQLPIAATGASLDTVFAEEMLGGGYRKKYLRAI
                     SRLNALTHSVRGGSGEG"
     gene            complement(12360..12956)
                     /locus_tag="VN041_01095"
     CDS             complement(12360..12956)
                     /locus_tag="VN041_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWU27676.1"
                     /translation="MIDLEKVNGDWEFDPAHTRIGFSAKHAMVTTVRGAFNEVSGHLH
                     VDTEHPENSWAKVTMKTGSVDTRVQQRDDHLRSADFFDSEKWPEITFVSTHIEEVDDR
                     AFSVTGDLTIRDVTKQVMIPLALIGIETGPAGELRAGFEGTRRLNRRDYGLEWNTPLD
                     SGGVLVSEKITLEFEISAVKTAGDDASGENGEGTDSEE"
     gene            13171..14406
                     /locus_tag="VN041_01100"
     CDS             13171..14406
                     /locus_tag="VN041_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWU27677.1"
                     /translation="MDSTGNISWALTATALVLLMTPGVAFFYGGLVRAKSVISMMMMS
                     FGAIGLIAVLWMLYGYSMSSVDGPGQFAGDPFKHFGLAEAASGPGANAALLSVAYGAT
                     FAIITVALISGAIADRARFGPWLIFVGVFGTIGYFPIAAWVWGGGWIMHLGTTLFGKD
                     SGIAVIDYAGGTAVHINAGAAALALALVLGKRIGFGKGIQKPHNVPLTLLGAALLWFG
                     WFGFNAGAEWLSPGMGHVGLIAVNTLGASAAGILGWILIEKLRDGKPTSVGAVSGLVA
                     GLVAITPACANLTPGWSLLLGLLSGAACALAVELKFRLGFDDSLDVVGLHLVGGVLGT
                     VYLGFFATGQGLFTGGDLRLLAVQIIAAGGVLVYSFLVSLAIGFAIEKTIGFRVTKEA
                     EVAGVDTFVHGEEGYALVE"
     gene            14427..15017
                     /locus_tag="VN041_01105"
     CDS             14427..15017
                     /locus_tag="VN041_01105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWU27678.1"
                     /translation="MSSTQRILSVLADLVGRLLAKEDPKRPTRPRHPDAVLRPDGRPS
                     AGSSRQTDEVSPGRTRGSETIEVDPDRVGDVNVGYSPSRNGQPDSGEVIWTWVPYEEN
                     DGRGKDRPVLVIGRQSADRVYAVRMTSKAHDRDRDFLSIGSGAWDSQGRESWVDIEQL
                     YSVHHDGMRREAAVLDKGRYGRVTEALIRRYGWAKV"
     gene            15116..15502
                     /locus_tag="VN041_01110"
     CDS             15116..15502
                     /locus_tag="VN041_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HWU27679.1"
                     /translation="MRFIPTKVHGVLDYLVGIALILAPWLFGFAAMGGPAVVIPIVLG
                     VGLIVYSLLTRYEWGPVKLIPMPVHLVFDVVASVFLAASPWLFGFASAPPNAWVPHVV
                     VGITVIIVVIFSEPQPARTELRTTRA"
     gene            complement(15605..15745)
                     /locus_tag="VN041_01115"
     CDS             complement(15605..15745)
                     /locus_tag="VN041_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HWU27680.1"
                     /translation="MRLGLGRAAADSLILSDVDAAPRALLGSGFSFRHQTAADAVDSA
                     LG"
BASE COUNT         2310 a         5350 c         5568 g         2519 t
ORIGIN      
        1 cttcgtgtcg gtgctcgcag gcgacaccgc cgctcagacc cgcgcgctcg cgcaccgggc
       61 cgtgcacgag catcccgacg gcgtggtcgt cgtgggcggt gacgggacgc tcaccggcat
      121 catcgacgtc ctcgccgatg cctacatgcc ggtgactctc gtgcccgccg gcacggggaa
      181 cgacctcgcc cgcgcgctgg gcatccccac cggcgatccc gccgccgcgg cgtcagccgc
      241 agtcgagggg agcccgcgtc gcatcgacct cggcagcatc gcctcgcagg gggagagccg
      301 ggcgttcctc acgatcgccg ccctcggttt cgacgccaag gtcagtgagc gcaccaacac
      361 gctgcggcat ccccgcggcc cgctgcggta ctacctcgcg ctggtgatcg agctgctgcg
      421 gctgcgaccc accgacttcc gggtgcgcgt cgacggcggc gaggagcagt cgattcccgg
      481 cacgctgctc gccgtcggca acacccccag ctacggggga gggatgccga tctgcgtcgg
      541 agccctgccc gacgacggcg cgctcgacct cgtgcacgtc gcaccgctcg gccggctgcg
      601 gctgctgacc gtgttcccgc tgctgctgcg cggcagccac ctgtcacgac cggaggtgca
      661 gcaccggcgg gcgacacgcg tgcaggtgtc ggctcccgac ctggtggtct acgccgacgg
      721 cgagcgcgcc ggcgcgggcg agtgcgtgat cggcatccga ccgggtgctg tgaccatgat
      781 ggtgccgcga cgatgaggac tccgaaggga tgaacggatg agctacgacg aggacgtcgt
      841 gatcgtggga tcgggcttcg gcggctcggt cgccgccctg cgcctgtcgg agaaggggta
      901 ccgcgtccgg gtgctcgagt ccggccgccg gttcgaggac gaggacttcg cgaggacgtc
      961 gtgggacgtg cgccgctacc tgtgggcgcc gcgactgggc tgtttcggcg tgcagcgcat
     1021 ccaccggctg ccgcacgtga tgatcctcgc cggtgcgggc gtcggaggcg gttcactgaa
     1081 ctacgccaac acgctctacg agccgggtgc ggcattcttc gccgatcggc aatggcccca
     1141 ggggacgtcc tggcagaccg agctcgcgcc gcactacgcg acggcgaagc ggatgctggg
     1201 cgtcgtcgag cactatccgc acaccggacc cgtggagcgc atcatggccg gcgccgccga
     1261 ggacctcgga gtcggtgaca ccttccggcg tgcgccggtg ggcgtcttcc tcggcatgcc
     1321 gggcgtcacg gtcccggacc cgttcttcga cggagagggt cctgcgcgga ccggctgcac
     1381 gctgtgcggc aactgcatgg tggggtgccg cgtcggagcc aagaacacgc tgcggaagaa
     1441 ctatctgcac ctcgccgagc gccggggtgc ggtgatcgag ccgctgcgca cggtcgtcga
     1501 ggtgcgtgag gatccgcgcg gcgggtatct cgtcacttct gagcgctccg gggcgtgggc
     1561 gcgccggcag cgccgtacga tccgcgcgga gcaggtggtg ctcgcggccg gcacgtgggg
     1621 gacgcagcag ctgctgcacc ggatgaaggc gtcgggcgcc ctgccgcgca tctcggatgc
     1681 cgtgggccgc ctgacccgca ccaactccga ggcgctggac ggcgcgatct ccacccgcgt
     1741 cccggagagc gtcgcgtaca cggagggcgt ggccatcacc acctctttcc acgtggacga
     1801 tcacacgcac gtggagaacg tgcgctacgg cgcgggctcc aatctgatgg gcggcctcgc
     1861 aacgctgctg gtgcatggcg accgctccct gttcgggcgt ctgggctcga tggccgcaca
     1921 ggtgctgcgg catccggtgc gccagttccg gctggcgaat ctgcaccggt ggagcgagcg
     1981 cggcatcatc gccctcgtca tgcagacggc cgacaactcg ctcacgctct cactgcggcg
     2041 tcgtctggga cgcgacgtgc tgaccagcag gcaggggcac ggggagccga atccgtcgta
     2101 cctgccgcag gcgcatcgcg ccgcggaggc tattgccgcg cggatgcagg acgagaccgg
     2161 agtgcgcacc gaggcgcggg gctcatggcc cgaggccttc ggcatccccc tgaccgcgca
     2221 cttcctcggc ggcgcggtga tcggcgcgac ccccgaggac ggcgtcatcg acgcctggaa
     2281 ccgcgtctgg gggcatccgg gcgtgcatgt cgtcgacggt gcggcggtgc cggcgaaccc
     2341 gggcgtgaac ccctcactga ccatcaccgc catcgccgag cgggcgttcg cgcactggcc
     2401 gcaccgcggc ggacaggatc agcgcccggc gcaggacgag gcgctcagtt gagggcgtgc
     2461 agggccgcga cggcgtccgg tgcgctatcg ccggcccgga gggcgccggc cagcaggtag
     2521 ccgtccgtca gagagtcacc ggtgatcatc acccgcacca gctccacacc gcacgcgacg
     2581 cgcaccgatt cggcgcaggc ggcgagcacc ctggtgctga tctcgtcgtt gtgcgggagc
     2641 tcttcgccgt ccagcatccg caccgcgtcg cgcagagcgg caccgacgat gttcgacgcg
     2701 cgttcccgca cgttcaccac gtcgagctcg aacaccctta ggtcgtgccc ggtcgggatc
     2761 ggccgccact cgaacgatgc gtgcactcgg tgctgcacgc cgcctgcgcc ggtcagctcc
     2821 cgtaccggca gatcggtcgc ccggcagcgc acgtccacga gccggtacca gtagaacagc
     2881 gggaatgccc cgtgcgaacc cggtcgcagc acgacgacct cgcccacggc accgctgagg
     2941 ctcgacttgc gccgtggcgg ccggttgcgg atgatcggct tgccgttccg ggtgcggatc
     3001 gcggcccagc cctcgtccac tgtcacgacg aagagcttca tcagcgccgg gaagaccagc
     3061 acgatcgtga ggatcgcggt tccggtcttc agcaggctcg cgaccccctt gccgctcacc
     3121 atcgtcagga gcatctccat gagaccactg tgccgggtct gtgcggatgc tgtaccccga
     3181 tcgagcctcg tcgagcagga gttcacgggg tgttcgccgt ccgttccccg cagacgcaga
     3241 acgggccgcc ccgaagtcgg agcggcccgt caggcggcgt tcgtcagctg tgcgcgatcc
     3301 cgcggatcgg cgggttgtgg aaggtgccct ggaacacgcg ctccgaggct ccctcgcggt
     3361 cgaggtacgg cgaggcgccg ccgtcgatga acggccagcc cgcgcccagg atcaggcaca
     3421 ggtcgatgtc ctcgacctcc gggaccacac cctcatcgag catgatccgg atctcctgcg
     3481 ccaggccgtc ctgcacgcgg cgcaggatcg tctccgcgga tgccggagtg gagccggtct
     3541 gacccttcag cgccttctcg gcggccttgg tccagccggt cacgcgaccg cccttgtcct
     3601 tctcgacgac cgcatccagt tcggcgaggg cgtggaagtt gtcgttcgcg tagaagcgct
     3661 ccgggaaggc gcggaccatc gtgtcctgca cgtgcgcggc gaccttccag ccgaccaggt
     3721 cgatcagctg gaacggaccc atcggcaggc ccaggggacc gaatgccttc tcgacgtcgg
     3781 cgacaggggt gccctcgtac acggcgcgcg cggcctcgcc catgaccttc gcgagcagac
     3841 ggttcacgac gaagccgggg gcgtcggccg tgagcaccgc gttcttgctg aggcccttcg
     3901 cgaccacgaa cgcggtcgac agggcagcat ccgtcgtggc gtccgtcttc acgatctcga
     3961 tcagcggcat gaccgcgacc gggttgaaga agtggaagcc gaccagacgc tcggggtgcg
     4021 cgagcttcga accgatctcc tcgacggaca gcgacgaggt gttggtcgcg aggatcgcgt
     4081 cctccgcgac gatcttctcg atctcgccga acacggcctg cttcacgccg acctcctcga
     4141 acacggcctc gattacgaag tcgcagtccg catagaggct cttgtccgtg gtgccggtga
     4201 ccaggctgcg cagcttgttg gcggtgtcgc cgtcgagacg gcccttcgcc tcgagcttgc
     4261 cgatctcctc atggatgtac gccacgccct tgtcgacgcg cgcctggtcc aggtcggtga
     4321 tgagcacggg cacctgcagc ttgcgcacga acagcagtgc gaactggctg gccatcaggc
     4381 cggcaccgat gatgccgacc ttggtgacct tcttcgcgag cgtcttgtcc ggtgcaccga
     4441 cggggcgctt ggcgcgcttc tgcaccaggt cgaacgcgta catcgacgcg gcgaactgat
     4501 cgccggtcac gagctcggcc agtgcctcgt cctcgcgggc gaagccctcc gccttcgtgc
     4561 cgctcctggc cttgtcgagc agctcgagcg cggcgtacgg tgacttcggc acggtgccga
     4621 tcttcgactc cagcatggtg cgggccattt tgatggcaac cggccacttg accgtgcgct
     4681 cgatcttgcc cggggcgttc ttgcgctcca ccttcttgcc gccgagaacc gcatcggccc
     4741 aggccagcga gttctccagg aagttcgcgg cggggtagat cgcgtcgaac aggcccagct
     4801 cgaacgcctg ctgcggcttg agcatccggt tctgcttgag cgggttggag atgaccacct
     4861 cgagggcgtt ctcgatgccg atcaggttgg gcaccagata cgcgccgccc cagcccggga
     4921 tgatgccgag gaagacctcg ggcagcgcga gcgctgcggc cgaggcgtcg acggtgcggt
     4981 acgtggagtt cagcgcgatc tccatgccgc cgccgagagc gagtccgttc acgaaagcga
     5041 aggacgggac gcccaggtcg gacagcgagc cgatgacctt gtgacccaac tgcgcgatca
     5101 ggcgtgcgtt gtccttcgac ccgacccggc tgatgtcgga gaggtcggcg ccggcggcga
     5161 ggatgtactg cttgccggtg atgccgaccg cctggatctc gccggcggcc gcacgggcct
     5221 tgagcccgtc gagggtctcg cccagctcgg tgagcgtggc gggtcccagc gtgttcgggc
     5281 gggtgtggtc gcggccgttg tccagggtga tcagggcgag caccttgccc gaggcgaggc
     5341 ggatgtcgcg cacgggggag tgcgtgacga cctcgccctc ggtgagggcc atgatcggcg
     5401 agtagtcgat gtcgtcgtag ttcgtcatgt ccgccgtcac ttccgcttct tgccgtcgta
     5461 gtacgggttc tcccagatga ccgagccgcc ctggccgaga ccgacgcaca tggccgtcag
     5521 gccgtagcgc acgtcggggc gctccgcgaa ctgcgcggcg agctggatca tcaggcgcac
     5581 accactcgcg gcgaggggat gcccgagggc gatcgcgccg ccccactggt tgacgcgtgg
     5641 gtcatcgtcg gcgatgccga agtggtcgag cagggagatc acctggatgg cgaacgcctc
     5701 gttgagctcg aacaggccga tgtcgtcgat ggtgaggccg gccttcttga gggccttctc
     5761 ggtggacggg atagggccga tgcccatgat ctcgggctgc acgccggcga aggcgaagga
     5821 gaccatccgc atcttcgggg tcaggccgaa ctccttgaca gcaccgccgc cggcgagcag
     5881 cgacatcgtc gcgccgtcgg tcagggggga ggaggtgccg gcggtgacgc ggccgtgcgg
     5941 acggaacggg gtcttcagcg ccgccaggtc ctccatggtg gtctgcgggc gacgcccctc
     6001 gtcctcggtc gcgaggcccc aggcgccgtc ggcgtccttg atcgccacgg acaccaggtc
     6061 gggctggatc ttgccggcgt cgtacgcagc ctggaccttg tgctggctga gcatgccgaa
     6121 gcggtccgag cgctccttgg tcaggtgcgg gaagcggtcg aagatgcgct ccgcggtgac
     6181 gcccatgttg agagcgcccg ggtccaccat gcgctccgcg acgaaccgcg ggttcgggtc
     6241 ggcgttgccg ccgatcgggt ggtgacccat gtgctcgaca ccgccggcga gggcgaagtc
     6301 gtacatgccg atgccgatgg aagccgccat ggtggtgacg ctggtcatgg cgccggcgca
     6361 catccgctcg acggcgaggc cgggaacggt ctgcggaaga ccggccagga tcgccaccga
     6421 ccgtccgagc gtcaggccct ggtcgccagt ctgggaggtc gcggcgatcg cgacgtcatc
     6481 gatgcggtcg gcagggacgg aggcgttgcg ctccatcagc ccgatcgtcg ccttgacggc
     6541 gaggtcatcc gcgcgggtgt tccaatacat tcctttttcg ccggcgcgcc cgaaaggcgt
     6601 gcgcactcca tcgacgaaga agacgtccga gatctcggcc actcctgcct ccattggttg
     6661 ggatgacccc agcctaggag ggcgttcgaa gcgtcaggaa tcggttggat cgaacctacg
     6721 aagccgtttc gggcgcgtcc gaatcggttt gcggcccctc tacaaaggcg tccgcgatct
     6781 tctgtgcggt atgcgcaacc tgccaccgtc gagccccgag cgcggcgaga gcctcgccga
     6841 cggcctccgc cgtcagcgcc ggcggctgcc aggccacccg gcgcaggaag tccggggtca
     6901 gcaggttctc cgtcggcatg tgcagctcct cggcgatggc ctccaccacg gggcgggcgg
     6961 ccttcagccg cagatcggcc tcggggttgc gatccgccca tgcgcgcggc ggggggagcg
     7021 catcgctggg cacgcgctca cgagggaggt cctccgtggt gcggccgcgc tcgatcgcat
     7081 cccaccatcg atccagctgg gtgcggctgg cgcgtccgtt gaactccttg agcgctgcga
     7141 gcgccgactt gctctgcggc gcggcgagca cggccgcgag cagggagcga tccggcacga
     7201 ggcggccggg ggagacgtcg atctcctgcg cgtacgcctc gcgtgcggtc cagagttcgc
     7261 gggcgaccgc caactggcga gcgccgcgca ccttgtgcag tccgctcaac cggcgccacg
     7321 gctcggtgcg aggggccttg ggctccttgt cgagagtcgc ctggaactcc tccgccgcga
     7381 tctcggtctt gcccgcctcc gcgagctcgc gggcgagcac gtccgcgacg tcgatcaggt
     7441 gcagcacgtc gagagcggcg tactcgagcc acggctgcgg gagcggccgc gtggaccagt
     7501 cggcggcgga atgcgccttg gccagggtga tcccgagggc gtgctcgacg acggcggcca
     7561 gaccgaagcc gtcgctgccc aggaggcggg atgccagttc ggtgtcgaag atgcggggcg
     7621 ggttcaggcc cagttcacgc agcgagggca gatcctggct ggcggcgtgg aagacccact
     7681 cggtgtcgcc gatggcatcc tgcagcgcgc tgaagtcgcc gacggccggc ggatcgaaca
     7741 ggaagacacc ggcgtcgcgc cggaagacct gcacgaggta cgcgcgctgc gagtagcgga
     7801 aaccggatgc ccgctccacg tcgacagcca ccggcccctg gccctgggag agggcgagac
     7861 acgccttctc gaactccgtg cggtcaccga tcacggagta ttcagccacg accgtccctt
     7921 ctcgcgccga acacggcgac atcctcggaa cccggcggga gacccgccag catccccacc
     7981 agctctgccc atgcttccac atgcgggcgc agcggcgcat ccggggtcca tgacgcgcgc
     8041 agctcgatct gggcgccgtc accctcctca gccaggccgc cgaagccctt cgagagcatc
     8101 ttcgtcgagg tccccgactc ggaatggtac tgcgcgtcgc gggagtccag ggcatcgacg
     8161 agccacgacc aggcgacgtc ggcgagcagg ggatcggtgc cgatctcggg ctccagcggg
     8221 gcctgggcga acgcgatgac gcgccaggcg ccgccccacg ccgcgggctc gtccggatcg
     8281 tgcagcagca cgaaccgtcc ggtgccgtag atggagtcgc cgtgatcatc cgggcgcacg
     8341 tccgcggcga gggccagcga gaagggcgcc agacctgcgg gggacgggat ctcccgcacg
     8401 acgatgtcgg cgcggaacgt cgtctcgcgc agctctgcgg cggcgacggc gaagagcgct
     8461 gcggcatccg ggtgtgcagt cacgtggaca ggctagagtc aggaggcgat gaagagtctc
     8521 aggcacgccg tagccatcgc cgtacccgcg ctgctggcgc tgggagccgc agccggagct
     8581 gtcgccggag cggccgtgtt cacggtcgcc cgccgtgtgg tcacgccgac ggtgcgacgc
     8641 aacgacacgc acatcctggc ggtggacacc ggcgcgcaga cgatcgagct ggatcgcacg
     8701 ccggacaccg agctgcccgg acgctacggg ctgttcacgt cgggaaccta cggctatgtg
     8761 aagctgggcg cggtactcgg cgcggattcc cggaccgtgc gacgcaagct gctgacccag
     8821 atcgaaccgg gtgcgcaggt ggacagggac gccgcgttca gcggctggta ctacgtgacc
     8881 ccgagcgagc tgcacctgcc gtgggagagc gtgctgatcg gctcatccgc gggcccgtgc
     8941 cccgcgtggt tcttccccgc ggcggacacc gggaacgccg acacgtgggt catccaggtc
     9001 catggtcgcg gcaccacccg atccgagtgc ctgcgcgccg tgccggtgtt ccacgccctg
     9061 ggcctgccga acctcgtcgt gtcctaccgc aacgacggcg aggcaccacg ctcccgcgga
     9121 gggagctatg cgctcggcgc gagcgagtgg cgcgacgtgg atgcggccat cggatacgcg
     9181 ctgcggcacg gagcgcggcg tgtggtgctg atgggctggt cgatgggcgg tgcgatcgcg
     9241 ctgcagaccg ccgtctcctc cggcaaccgc gaccggatcg cgggtctcgt gctggactca
     9301 ccggtcgtcg actggcgcac cgtgctgcgc ttccaggcgc gcgaggaggg cctgcgagaa
     9361 ccgcttccgg ccctggccat gggcgtgctg gagaacacgt tcacggcgcg gctcagtggc
     9421 gcggagaacg cgatccgctt cgatcgcctg gacatggtgg cacgggcatc cgagctgcac
     9481 gcaccgatcc tgatcctgca cagcgacgac gacggattcg tccccgcgga tgcctcccat
     9541 gcgctggccg ccgcgcggcc cgacctcgtg accatgcccg cgttcgcggt ggcgcggcac
     9601 acgaagctct ggaactacga ccagacgcga tggacggccg cgatcaccga ctggatgtcg
     9661 gagcacggcc tcggctcgcg cgccggctcc tgagccgggt cagcgcttct cggcgacctg
     9721 ccgctgggcg cgcaccagca tcccggtcat gccggcgatc cgcaggggcg agaccgactt
     9781 cgtcaggccg atggtctgag ggtagtcgcc cgggatcgcg agcacttcgt cggcggtgag
     9841 gccggtgagg ccctgcacca ggatgctcgc gaacccgcgg gtggtcggtg cctccggcgg
     9901 cgccgtcgcg tgcatggcga cgacgccgtc ggtgacctcg acgttgatga acacggggga
     9961 ctggcactcc gcgacgcgct cgtatatgtc gacgttgccg gcgaggtgct ccggcaccgc
    10021 cgggagctcg gacgagtact ccaggagcag ctccaggcgg tcctcctgag gaagctccag
    10081 gaactcctcg cggacctccg ccaggcgtgc gggaagatgc gtgtcagtca cccaccgatt
    10141 ctcccacgtt cagagcgagc cgggctcggt ccccgtgacg atcggaacgc gcacggcgct
    10201 gccccactcg gtccacgagc cgtcgtagtt gcgcacgttc tcgaagccga gcaggtgctg
    10261 caggacgaac caggtgtggc ttgagcgctc accgatgcgg cagtaggcga tcacgtcgtc
    10321 gccggcctgg aggcccgctc cgtcctgata gatctcctcg agctcggcgc gggtcttgaa
    10381 tccgccgtcc tcggcgaccg cacggctcca cggcacgctc ttcgccgtgg ggatgtgacc
    10441 gccgcgcagc gagctctcgt cggggtaggc cggcatgtgg gtgcgctcac cggagtattc
    10501 ctcgggggag cgcacgtcga tcagcggccc ctggccgatg aacgccagca cgtcgtcctt
    10561 gtacgcgcgc agcacggagt cgtcgcgctc gaccacggga tactccgtcg caggacggac
    10621 ggtcttctcc gtggtgatct cacggccctc ggcgatccac ttgtcgcgac cgccgtcgac
    10681 cagccgcacg tcctcgtggc cgaagagcga gaagacccag agtgcgtagg cggcccacca
    10741 gttgttcttg tcgccgtaga tcacgacggt gtcgtcgcgc gagatgccct tgcggctgag
    10801 cagttcggcg aagcccttgc cgtcgacgta gtcgcgcacg acggggtcgt tgagctcggt
    10861 gtgccagtcg accttcacgg cgcccgggat gtggccggtc tcgtagagca gcacgtcctc
    10921 gtcggattcg acgacgacaa ggccgggtgc gccgaggttg gcggcgagcc actcggtggt
    10981 gacgagtcgg ccgggtgcgg cgtactcggc gaacttggcg gatgagttgt cgtgctcgac
    11041 ggtcacggga tgctcctctt ggttcaggac atggatgccg gtggcggacg gtgcagggct
    11101 gcacgggttc gttcaggccg cgcacgctgc tggcggatgc tgccgggttg cgccccgttg
    11161 attcaggacg cgggggtggg tatgcgacgg cgtaaagttg agccgtcccc tttgacgata
    11221 gatcccttcg gtcgggcccg catccggatc gtaagactat gacttctgac ctcaggaatg
    11281 tgatgaccca gcagacgagc acgggtgtcg tgcacctcac cgagcgcaca ccgatcgtca
    11341 ccggcccgga gatgctcgcc agcctcgtgc cgccgccgca gttcgactcc gcgaccttcg
    11401 acagctaccg cgccgacgac gcctatccgt ctcaggaggc ggcgaaggag acgctgcagc
    11461 ggttcgcggg tcgcagcgag ccggtgaaga aggtcgggct gttccgccgc gcgcccaagg
    11521 aggccccgat gaagccgggc gtgtacctgg acggtggctt cggcgtcggc aagacgcacc
    11581 tgctggcatc cgtgtaccac gcgatgccgt cccgcaggaa gtacttcggc tcgttcatcg
    11641 agtacaccgc actggtcggc gcgctgggct accgcaacac cgtggatctg ctgcgcggct
    11701 ccgatctgct ctgcatcgac gagttcgagc tggacgatcc cggcgacacg atggtgatga
    11761 cccggctgct gggggagctg gtggcctcgg gcaccaagct cgccgccacc tccaacacgc
    11821 ctccgaacgc gctcggcgag ggccggttcg ccgcgcagga cttcctgcgc gagatccacg
    11881 cgatgtccga gagcttcgag actctgcgca tcgacggggt cgactaccgt cagcgcgcga
    11941 tcgacggcag cgcggtcgtg ctggacgacg acggctacgc gaaggccatc gcgggcgccg
    12001 ccgcggccgg cacggcgtcg gacgacgcgt tcgccgatgt catccggcac ctcgcgcagg
    12061 tgcatccctc gcgttacatc cgtctgatcg acgggctcga tctggtgggc ctgcgcgacg
    12121 tgcaggtgct caccgatcag tccgaggccc tgcgcttcgt ggcgttcgtg gaccgtgtgt
    12181 acgacgccca gctcccgatc gcggccacgg gcgcgtcgct ggacaccgtg ttcgccgagg
    12241 agatgctcgg cggcggatac cgcaagaagt acctgcgggc gatctccagg ctcaacgccc
    12301 tcacccactc ggtgcgcggc ggctccggcg agggctgacc cccgccggag caccgccggt
    12361 cactcctcgg agtcggtgcc ctcgccgttc tcgccggatg cgtcgtcgcc tgcggtcttg
    12421 accgcagaga tctcgaactc gagggtgatc ttctccgaca cgagcactcc gccggagtcg
    12481 agtggcgtgt tccactccag accgtaatcg cggcggttca ggcggcgggt gccctcgaag
    12541 ccggcgcgga gctcgcccgc ggggcccgtc tcgatgccga tcagcgccag cgggatcatg
    12601 acctgcttgg tcacgtcgcg gatggtgagg tcgcccgtga cgctgaacgc gcggtcgtcg
    12661 acctcctcga tgtgggtgct cacgaaggtg atctccggcc acttctccga gtcgaagaag
    12721 tccgcgctgc gcaggtggtc gtcgcgctgc tgcacgcgcg tgtcgacact gcccgtcttc
    12781 atcgtcacct tcgcccagga gttctccggg tgctcggtgt cgacgtggag atggccggag
    12841 acctcgttga acgcaccgcg gacggtggtg accatggcgt gcttcgcgga gaagccgatc
    12901 cgggtgtgcg ccgggtcgaa ctcccagtcg ccgttgactt tttcgagatc gatcacacga
    12961 gactcctcat tcggactccc gtcaggtggc gggagaaacg tcctcctgcc tgggggctgg
    13021 aggtggattc gagaataccc gaactgcttc cggtaaccgg atcgtcatct ggcggcatcc
    13081 gctgtaacac gttcgcaaca cgcagcacgc gagatcgaaa cacgtcgcgc ccagacttct
    13141 ttcatccgac gaagagagaa gggtgcggac atggactcca ccggcaacat ctcgtgggcg
    13201 ctcaccgcca cggcactcgt gctgctgatg acgcccgggg tggcgttctt ctacggcggc
    13261 ctggtgcggg cgaagagcgt catcagcatg atgatgatga gcttcggtgc gatcgggctc
    13321 atcgccgtgc tgtggatgct gtacggctac tcgatgagca gtgtcgacgg ccccggccag
    13381 ttcgcgggtg atcccttcaa gcacttcggg ctcgcggaag ccgcctcggg ccccggagcc
    13441 aacgccgcgc tgctgagcgt cgcatacgga gccaccttcg cgatcatcac cgtcgcgctg
    13501 atctcgggag ccatcgccga ccgtgcgcga ttcggtccgt ggctcatctt cgtgggggtg
    13561 ttcggcacca tcggctattt cccgatcgct gcctgggtct ggggcggcgg ctggatcatg
    13621 cacctgggca cgaccctgtt cggcaaggac agcgggatcg cggtgatcga ctacgccggc
    13681 ggcacggctg tgcacatcaa cgccggcgcc gccgccctgg ccctggccct ggtgctgggc
    13741 aagcgcatcg gcttcggcaa gggcatccag aagccgcaca acgtgccgct gaccctgctg
    13801 ggcgccgcgc tgctgtggtt cggctggttc ggcttcaacg ccggggcgga gtggctgtcc
    13861 ccggggatgg ggcacgtcgg gctgatcgcg gtgaacacgc tgggggcgtc ggctgcaggc
    13921 atcctgggct ggatcctcat cgagaagctc cgtgacggca agccgacgtc ggtgggcgcg
    13981 gtctcgggac tcgtcgccgg actcgtcgcg atcacgccgg cctgcgcgaa cctcacgccc
    14041 ggctggtcgc tcctgctggg tctgctctcc ggtgccgcgt gcgctctggc cgtggagctg
    14101 aagttccgtc tcggcttcga cgactcgctc gacgtcgtgg gcctgcacct tgtcggcggc
    14161 gtgctgggaa cggtgtacct gggcttcttc gccaccggtc agggcctgtt caccgggggc
    14221 gacctgcgtc tgctggcggt gcagatcatc gccgccgggg gagtgctggt gtactcgttc
    14281 ctggtgtcgc tggcgatcgg attcgcgatc gagaagacca tcggcttccg ggtcacgaaa
    14341 gaggccgagg tcgcgggtgt cgacaccttc gtgcacggcg aagagggcta cgcgctggtc
    14401 gaatgagtcg cggctagggt gtgggggtga gctccactca gcgcatcctg tccgtcctcg
    14461 cagatctcgt cggccgactg ctggccaagg aggacccgaa gcggcccacg cgcccgcggc
    14521 atccggacgc cgtgctccgc cccgatgggc ggcccagcgc cggttcgtcg cgtcagaccg
    14581 acgaggtctc accgggacgg acgcgcggtt ccgagacgat cgaggtggac cccgatcggg
    14641 tcggcgatgt gaacgtcgga tactcgccct cccgcaacgg tcaaccggac tccggcgagg
    14701 tgatctggac ctgggtgccg tacgaggaga acgacgggcg cggcaaggac cgtccggtgc
    14761 tggtgatcgg aaggcagtcc gcggatcgcg tgtacgcggt ccgcatgacc agcaaggccc
    14821 acgaccgcga ccgggacttc ctctccatcg gatcaggtgc ctgggactcg cagggccggg
    14881 agtcgtgggt ggacatcgaa cagctgtaca gcgtgcatca cgacgggatg cgacgcgagg
    14941 ccgcagtgct ggacaagggg cgctacggcc gcgtgaccga ggcgctcatc cgcaggtacg
    15001 gctgggcgaa ggtctgagga gcggtccgcg ctccgcgggc atccaatccg atcggtgcag
    15061 ggtgacgaac cctcagtgag ccgctttccg cggcgcatcc gatcgaggag gaatcatgcg
    15121 tttcatcccc acgaaagtcc acggcgttct cgactatctc gtcggcatcg ccctgatcct
    15181 cgccccctgg ctgttcggct tcgccgccat gggcggcccc gccgtcgtga tccccatcgt
    15241 cctgggcgtc ggactgatcg tctacagcct gctgacacgg tacgaatggg gtccggtcaa
    15301 gctgatcccg atgcccgtgc acctcgtgtt cgacgtcgtc gcgagcgtct tcctcgccgc
    15361 atccccctgg ctcttcggct tcgcgtcggc tccgccgaac gcctgggtcc cccacgtggt
    15421 cgtcgggatc acggtgatca tcgtggtgat cttctccgag ccccagcccg cgcgcaccga
    15481 gctgcgcacg acgcgggcct gaggcattca cgatcgcacg tccgaagggg cgtcagagcg
    15541 gagccgtccc tgacgccccg taggcgtcac gagatggagt cgccggtgcg gcggatgccg
    15601 tggatcagcc gagcgccgag tccacggcat ccgcagcggt ctgatggcgg aacgagaagc
    15661 cgctgcccag cagcgctcgg ggcgccgcat cgacgtcgga gaggatgagc gagtcggccg
    15721 ccgcgcgtcc cagccccagc cgcagcg
//
LOCUS       DATMSX010000033         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_104729,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000033 DATMSX010000000
VERSION     DATMSX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104729"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..823
                     /locus_tag="VN041_01120"
     CDS             47..823
                     /locus_tag="VN041_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWU27681.1"
                     /translation="MNAAAQANLEGMYAAPAAGAIETDRMTVEDTIWKTAGLFGILLV
                     AAAIGWVWTVAGSAAGQANLTPWIVGALGGFVLAMVVIFTSRKKVRPALIFAYAAFEG
                     LFVGGISAFFEMLYPGVVMQATLATVAVVGVTLALFASGKVRASAKMTKIFMIAMFGY
                     MAFSLLNLILMWTGVSDNPWGLRGDIVIAGIPLGVIIGVFVVFLAAYSLVMDFDSIQQ
                     GVRNGAPRKYGWMGAFGIMVTVVWLYVEILRILAILRGND"
     gene            1001..1273
                     /locus_tag="VN041_01125"
     CDS             1001..1273
                     /locus_tag="VN041_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWU27682.1"
                     /translation="MSFLGFLLLGLIAGAIAKLILPGKQGGGWFVTLLLGVVGALLGG
                     WLGGLLFNVDLNGFWNLSTWLLAIGGSIVVLLIYGLIVGRGDRSRA"
     gene            complement(1353..2624)
                     /locus_tag="VN041_01130"
     CDS             complement(1353..2624)
                     /locus_tag="VN041_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWU27683.1"
                     /translation="MTAEGVARPASQDARRAVAAVWRIESAKIVATLTRETSDFALAE
                     DLAQEALAEALEQWPDAGVPHNPAAWLTAVAKRRAIDGWRRQERYDDRIAALAHDLER
                     EQDEASDALPWDPDAIDDDVLRLIFIACHPVLSREAQVALTLRVVGGLGTEEIARAFL
                     VPVATVQQRIVRAKKTLGAAHAPFELPPREEFGSRLSAVLGVLYLIFNEAHTASGGED
                     WMRPDLGREAVRLARVLAGLVPREPEVHGLLALMELTAARFPARVDRQGDPVLLADQD
                     RTRWDRSAIARGRAALARVDALGRGRGGYALQASIAECHAIAASVEQTDWDRIVMLYE
                     ALGRLTPSPVIELNRAAAVAMATGPASALRIVDSLIAAGELRGYHLLPATRGELLLRL
                     GRDDEARAEFAAAACLAGNDRERALLEAKAR"
     gene            complement(2626..3048)
                     /locus_tag="VN041_01135"
     CDS             complement(2626..3048)
                     /locus_tag="VN041_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWU27684.1"
                     /translation="MKYMLIMRATDEGVEAFKSIPFEQVIEAMGKYNESLIKAGVMAG
                     GDGLTDAEEGVVVDFSSEPPLVTDGPYGETKELFNGFWILDVASKEEAVEWAKRCPLG
                     PGSKLEIRRVTDVSDFPQDNEWIEKEKQWREDAAASEA"
BASE COUNT          484 a         1191 c         1105 g          563 t
ORIGIN      
        1 cccgcccggc gccgccggac agaccaccgc gttccagcag gcgggcatca acgccgccgc
       61 gcaggcgaac ctcgagggca tgtacgcggc tcccgccgca ggcgccatcg agaccgaccg
      121 catgacggtc gaggacacga tctggaagac cgccggcctg ttcggcatcc tgctcgtggc
      181 cgcggccatc ggctgggtgt ggacggtcgc cggctccgct gccggccagg cgaacctcac
      241 cccgtggatc gtcggcgcgc tcggcggctt cgtgctcgcc atggtcgtca tcttcacctc
      301 gcgcaagaag gtccgccccg cgctgatctt cgcgtacgcg gccttcgagg gcctcttcgt
      361 cggcggcatc tcggcgttct tcgagatgct gtaccccgga gtcgtcatgc aggcgaccct
      421 cgcgacggtc gccgtcgtcg gcgtcaccct cgccctgttc gccagcggca aggtgcgcgc
      481 cagcgcgaag atgaccaaga tcttcatgat cgcgatgttc ggctacatgg cgttctccct
      541 cctcaacctg atcctgatgt ggaccggcgt cagcgacaac ccgtgggggc ttcggggaga
      601 catcgtcatc gcaggcatcc cgctcggcgt catcatcggc gtcttcgtgg tgttcctcgc
      661 cgcgtactcg ctggtgatgg acttcgacag catccagcag ggcgtgcgca acggcgctcc
      721 ccgcaagtac ggctggatgg gcgccttcgg catcatggtg acggtggtct ggctgtacgt
      781 cgagatcctg cgcatcctcg cgatcctgcg cggcaacgac tgaccgtcga tccgcacgct
      841 ctgaacggcc gccctcctcg gagggcggcc gttctgctca tcacagcagg ctgatagcgc
      901 attcctgaac tcgtgcgcaa gggggtagct ctagacgctc gtctaaggca ttctggtcat
      961 gcccggacac cccggacgca cctacagaac ggagactccc atgagcttcc tcggattcct
     1021 tctcctcggc ctgatcgccg gcgccatcgc caagctgatc ctccccggca agcagggcgg
     1081 cggatggttc gtcacactgc tcctcggcgt cgtcggcgct ctgctcggcg gctggctggg
     1141 tggtctgctg ttcaacgtcg acctgaacgg gttctggaac ctgtctacct ggctcctcgc
     1201 catcggcgga tcgatcgtcg tgctgctgat ctacggtctc atcgtgggcc gcggggaccg
     1261 cagcagggcg taagaaccct tcttccgggg aggcgggtcg ggggatccgc ctcccctttc
     1321 gcatgcctgc aggagcggaa ccgcacgcgc gctcagcgtg ccttggcctc cagcagcgcc
     1381 cgctcacggt cgttgcccgc cagacaggcc gccgcggcga actccgcccg tgcctcgtca
     1441 tcgcgtccga gccgcagcag cagctcgccg cgggtcgccg gcagcaggtg gtagccgcgc
     1501 agctcccccg ccgcgatcag agagtcgacg atgcgcagag cggatgccgg gcccgtcgcc
     1561 atggcgaccg cagcagcacg gttcagctcg atgaccggcg acggcgtgag tcggccgaga
     1621 gcctcgtaca gcatcacgat gcgatcccag tcggtctgtt cgacggatgc cgcgatggca
     1681 tggcactcgg cgatcgacgc ctgcagcgca tacccgcctc gtcccctccc gagtgcgtcc
     1741 acccgcgcga gcgccgcgcg tccgcgggcg atggcgctgc ggtcccagcg ggtccggtcc
     1801 tgatcggcga gcagcaccgg atcgccctgg cggtcgacgc gggcgggaaa gcgcgctgcc
     1861 gtcagctcca tgagcgcgag caggccgtgc acctcgggtt cccgcggcac gagccccgcg
     1921 aggacacggg ccagtcgcac ggcctcccgc cccaggtcgg gacgcatcca gtcctccccg
     1981 ccgctggccg tgtgcgcctc gttgaagatc aggtagagaa ccccgagcac ggcgctcaga
     2041 cgagagccga actcctcgcg cggcggcagc tcgaacgggg catgcgctgc tccgagcgtc
     2101 ttcttcgcac gcacgatgcg ctgctgcacc gtggccacag gcacgaggaa ggcgcgggcg
     2161 atctcctcag tgccgaggcc cccgaccacc cggagggtga gcgcgacctg cgcctcgcgg
     2221 gagagcacgg ggtggcaggc gatgaagatc aagcgcagca cgtcgtcgtc gatggcatcc
     2281 ggatcccacg gcagagcgtc ggatgcctca tcctgctcgc gctcgagatc gtgcgcgagc
     2341 gccgcgatcc tgtcgtcgta ccgttcctgg cgacgccagc cgtcgatcgc gcggcgcttg
     2401 gcgacggcgg tcagccacgc tgccggattg tggggcacac cggcatccgg ccactgctcg
     2461 agagcttcgg cgagcgcctc ctgcgcgaga tcctcggcga gggcgaagtc ggatgtctct
     2521 cgggtgagcg tcgcgacgat cttcgccgac tcgatgcgcc acaccgcggc cacggcgcgc
     2581 cgcgcatcct gcgatgcggg gcgcgccacg ccttcggcgg tcatctcagg cctcggatgc
     2641 ggcggcatcc tcgcgccact gcttctcctt ctcgatccac tcgttgtcct gagggaagtc
     2701 ggagacgtcg gtcacgcggc ggatctccag cttgctgccg ggaccgagcg ggcagcgctt
     2761 ggcccactcc acggcctcct ccttcgaggc cacatccagg atccagaatc cgttgaacag
     2821 ctccttcgtc tctccgtagg ggccgtcggt cacgagcggc ggctcggacg agaagtcgac
     2881 gacgacgccc tcttccgcgt cggtcagccc gtcgccgccg gccatcacgc cggccttgat
     2941 cagcgactcg ttgtacttgc ccatggcctc gatcacctgc tcgaagggga tgctcttgaa
     3001 cgcctccacg ccctcgtcgg tcgcgcgcat gatcagcatg tacttcatgg tggttctcct
     3061 tgttcggtcg ttcatcacac gggccgtgtc tcgaccctct cattgaagac gtcgaacgag
     3121 aaggcgcggg atcgacatcc tcgcgagaat tcttcgagaa tcgcttcctg ccatgatggt
     3181 ggtcacggag gagggcccga tcacagatcg gcccgcgagc gggagggcag tccatgcgcg
     3241 gaccatgggg gaagatcact gcggtcgcga gcgcgatcgg cctggcggca gggctcgcgc
     3301 tgggcagcgc tctgctgtcc ggcggtgcgg catccgccgc cac
//
LOCUS       DATMSX010000034         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123728,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000034 DATMSX010000000
VERSION     DATMSX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123728"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VN041_01140"
     CDS             <1..419
                     /locus_tag="VN041_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWU27685.1"
                     /translation="AGGGSPERTRAVDFEAAVRSMDAARDAGVDRYVMVSYFGSSPEH
                     GIPVSDPFHAYAEAKAAADEYLRATSLDWTVLAPSRLTLGDATGRIDGTAAVSGEVSR
                     ANVALVIAETLQQPAAIRQTIRFNDGDRPIAEALAG"
     gene            complement(443..1732)
                     /locus_tag="VN041_01145"
     CDS             complement(443..1732)
                     /locus_tag="VN041_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HWU27686.1"
                     /translation="MSRTPMALLSPADQERLRGLRRMKAVALGALIVMAVVFVIAFAL
                     EHRYPWLAYVRAAAEGGMVGALADWFAVTALFRHPLGLPIPHTAIIPNRKDEIGRSLG
                     EFVETNFLSGEIVRDKLSRTALSKRLGEWLQDPAHAERVAAEGSTAASAVLRALSDDD
                     VQGLIADLAREHLIEPDWAPSAGGWLERIVEADAHRGAVDLAVDSISTWLHANAHTFQ
                     GLVSRRLPGWVPKLAHRFVDETVYHEAVRFVEAVQADPQHPARQALDGYLARLADRLQ
                     HDPAMRDRFEGAKAAVFDSPRVIGLAAQAWDTAKNGLLTALADPESGLRRRAASAVAE
                     IGRRLATEPALQARVDGWVTDAAVFLVDRYRHDIASIITDTVEKWDAAETTEKIELMV
                     GRDLQYIRLNGTVVGALAGLAIFAVAHAAIPVPLGLG"
     gene            1767..2039
                     /locus_tag="VN041_01150"
     CDS             1767..2039
                     /locus_tag="VN041_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWU27687.1"
                     /translation="MIDDIKKRALHRTSILEGQLRGIARMIENEDYCMDIITQSRAVQ
                     RSLESLNRLLLENHLRTHVTHMFEQGGDEREQAVGELLKAFDFDRK"
     gene            2175..>3174
                     /locus_tag="VN041_01155"
     CDS             2175..>3174
                     /locus_tag="VN041_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27688.1"
                     /translation="MADIAVAATAPDAGTASGGVEIAGVTKHYGESTAVDDLSLTIRP
                     GEFLSLLGPSGCGKTTTLRMIAGFEHPDSGDIRISGRSVLGLPPYRREVNTVFQAYAL
                     FPHMTVAENVAYGLQQRGVPKAEQRQRVAEALDMVQLRRFADRKPTMLSGGQQQRIAL
                     SRALINRPSVLLLDEPLAALDRQLREEMQVELKLLQSRLGTTFVFVTHDQGEALSMSD
                     RIAVMRAGRIEQLDAPDAIYAAPASAYVASFIGQQNFIRGIAADGDAIETPLGVIEGR
                     WGAGRVERGAEAVAAIRPEFIRLDSAGATGAGADGRVLSVSYLGETLQVVVGVADGQ"
BASE COUNT          481 a         1103 c         1113 g          477 t
ORIGIN      
        1 gcgccggcgg cgggagtccg gagcgcacgc gcgccgtcga cttcgaggcc gccgtgcggt
       61 cgatggacgc ggcgagagac gccggtgtgg accgctacgt catggtctcc tacttcggct
      121 cctcgccgga gcacggcatc cctgtatccg atccgttcca tgcctacgcc gaggcgaagg
      181 cggccgccga cgagtacctg cgggcgacca gcctggactg gaccgtgctg gcgcccagcc
      241 gcctgactct cggcgatgcc accggccgca tcgacgggac cgccgccgtc tcgggcgagg
      301 tgagccgcgc gaacgtcgcg ctcgtgattg cggagacgct gcagcagccc gcggccatcc
      361 gccagacgat ccgcttcaac gacggcgacc ggccgatcgc cgaggccctg gcgggctgag
      421 gccccggctc acgccaccgg catcagccca gtcccagcgg aaccgggatc gcggcgtgcg
      481 cgacggcgaa gatcgcgagt cccgcgagag cccccaccac cgtgccgttg agccggatgt
      541 actgcagatc gcgaccgacc atcagctcga tcttctcggt ggtctccgcg gcatcccact
      601 tctcgacggt gtcggtgatg atcgacgcga tgtcgtgccg gtagcggtcc accaggaaca
      661 cggcggcgtc ggtgacccag ccgtcgacgc gggcctgcag ggcgggttcg gtggccaggc
      721 gtcgcccgat ctcggcgacg gcggatgccg cacggcggcg cagcccgctc tccggatcgg
      781 cgagggcggt gagcaggccg ttcttggcgg tgtcccaggc ctgcgccgcg agcccgatca
      841 cccgcgggct gtcgaacacc gcagccttgg cgccctcgaa ccggtcccgc atggcggggt
      901 cgtgctgcag gcggtcggcc aggcgggcca ggtagccgtc gagcgcctgt cgggcgggat
      961 gctgcgggtc ggcctgcaca gcctccacga agcggacggc ctcgtggtag accgtctcgt
     1021 cgacgaagcg gtgcgcgagc ttcgggaccc agcccgggag ccgccgggag acgagaccct
     1081 ggaaggtgtg cgcgttggcg tgcagccagg tcgagatgct gtccacggcc aggtccaccg
     1141 caccgcggtg cgcatccgcc tcgacgatgc gctccagcca gccgcccgcg gacggcgccc
     1201 agtccggctc gatcaggtgc tcgcgcgcga gatcggcgat gaggccctgc acgtcgtcgt
     1261 cgctcagcgc gcggagcacg gcggatgccg cggtcgagcc ctccgcggcg acccgctcgg
     1321 cgtgagcagg atcctgcagc cattcgccga ggcgcttcga cagtgccgtg cgggagagct
     1381 tgtcgcgcac gatctcgccg gagaggaagt tggtctcgac gaactcgccc agcgagcggc
     1441 cgatctcgtc cttgcggttc gggatgatcg cggtgtgcgg gatgggcagt cccagtgggt
     1501 gccggaacag cgcggtgacg gcgaaccagt ctgcgagtgc gccgaccatg ccgccctcgg
     1561 ctgccgcgcg cacgtaggcc agccacggat atcgatgctc cagggcgaac gcgatcacga
     1621 agaccacggc catgacgatc agcgcgccga gcgccaccgc cttcatccgg cgcaggcccc
     1681 gcagccgctc ctgatcggcg ggggacagca gcgccatcgg ggttcgcgac atgccgtcat
     1741 cctttcatga cggctacgct ctcagtgtga tcgacgacat caagaagcgc gcactgcacc
     1801 gcaccagcat cctcgagggt cagctgcgag gcatcgcgcg gatgatcgag aacgaggact
     1861 actgcatgga catcatcacg cagtcgcgtg ccgtgcagcg ctcactggag tccctgaacc
     1921 ggctgctgct cgagaaccac ctgcgcacgc acgtgacgca catgttcgag cagggcggtg
     1981 acgagcgcga gcaggccgtc ggcgagctgc tgaaagcctt cgacttcgac cgcaagtagc
     2041 gtaaattccg ttgcctcgct aagtctcgac aacggatatc gtcgcgattc tgcttctttt
     2101 cttcgctttt cgctgtacct cagcacccga ctgtggttga ctgacggctg ttcgaatctc
     2161 atcgtggagg tttcatggcc gatatcgcag tggcggcgac cgcacccgac gcaggcaccg
     2221 cgtccggtgg cgtggagatc gccggcgtca ccaagcacta cggcgagtcg accgccgtcg
     2281 acgacctgtc cctgaccatc cggcccgggg agttcctctc cctgctcggt ccgtccgggt
     2341 gcggcaagac gacaacgctg cgcatgatcg ccggcttcga gcacccggac agcggcgaca
     2401 tccgcatctc cgggcgcagc gtgctgggcc tgccgcccta caggcgcgag gtcaacacgg
     2461 tcttccaggc ctacgcgctg ttcccgcaca tgacggtcgc cgagaacgtg gcgtacgggc
     2521 tgcagcagcg cggtgttccc aaggccgagc agcgccagcg cgtcgccgag gcactggaca
     2581 tggtgcagct gcggcgcttc gccgatcgca agcccaccat gctctcaggc gggcagcagc
     2641 agcgcatcgc gctgtcgcgg gcgctcatca accgtcccag cgtgctgctc ctggacgagc
     2701 cgctcgcggc gctcgaccgg cagctgcgtg aggagatgca ggtcgagttg aagctgctgc
     2761 agtcccggct cgggacgacg ttcgtcttcg tcacgcacga tcagggcgag gcgctgtcga
     2821 tgagcgatcg catcgccgtg atgcgggcag ggcgcatcga gcagctcgac gcgcccgacg
     2881 ccatctatgc ggcacccgcc tccgcctatg tggcctcgtt catcgggcag cagaacttca
     2941 tccggggcat cgccgccgac ggcgatgcga tcgagacgcc cctcggcgtg atcgaagggc
     3001 gctggggcgc cggtcgtgtc gagcgcggcg cagaggccgt cgcggcgatc cgtccggagt
     3061 tcatccgact cgactccgcg ggcgcgaccg gtgccggggc cgacggccgc gtgctgagcg
     3121 tgtcgtatct gggcgagacc ctgcaggtcg tggtcggggt cgcggacggg caga
//
LOCUS       DATMSX010000035         6723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_152056,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000035 DATMSX010000000
VERSION     DATMSX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152056"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VN041_01160"
     CDS             complement(<1..752)
                     /locus_tag="VN041_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27689.1"
                     /translation="MRLRPIAVASIALLAALSGCSTAPGADTSSPPPTVDGHGAIAGA
                     HELAEPALHLATVAPDGEVAHLDLLDETTEALTRTEPAVEVVTDGRYLFTIHDGAVTI
                     IDSGVWTWSHIDHFHYYEAPSRVVGEVKGSGTATVVPGETGVGILFADEAVLLETAAL
                     ADGDIVERFRIDVDEHDGLVVPIAEGALVTAADADGDPQELRILTAKGEERGRLACAH
                     AAGTITTVVGVVVGCADGALLAVGGDAAEPEIG"
     gene            complement(749..1579)
                     /gene="aztB"
                     /locus_tag="VN041_01165"
     CDS             complement(749..1579)
                     /gene="aztB"
                     /locus_tag="VN041_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HWU27690.1"
                     /translation="MSPLIEPFTAGFVQRGLTVGLLVALVCAVVGTWVVLRGMAFLGE
                     ALAHGMLPGVAAATLIGVPAILGAGVSASAMAVGVAALQRRGRLSADTSIGLLFVASL
                     ASGVIIISASRSFATDATAILFGDILAAGVDDVVQLAIVAALTLLVAAVGHRSFVALT
                     VDPRQAQLLGLRPRLAHILLVGMVTLAVVAAYQVVGSLLVVGMLLGPAVAAGAWASRI
                     VTTMAVAAGVGFASVAAGLLISWHAGTAAGSTVAFLAILAAPLSALLRHLIRPSERNT
                     "
     gene            1634..2344
                     /gene="aztA"
                     /locus_tag="VN041_01170"
     CDS             1634..2344
                     /gene="aztA"
                     /locus_tag="VN041_01170"
                     /inference="COORDINATES: protein motif:HMM:NF040873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HWU27691.1"
                     /translation="MSSAPRLLDTTRVLAFDDVHVIRDGHAALRGVDVEAHAGRLVAV
                     VGTNGSGKSTLLETAAGLLHPQRGAVWRRDGLRMTFVPQSTALPPHLPLTVADIVAMG
                     TWARLGLWRRPQRVDRAAIAEAIAMVGLRSQSNRRIGALSGGQRQRALLAQALVQRAD
                     LVLLDEPMAGLDAATRMVVGGAVDRLTADGAAVIVVTHDLDELARADEVLTLNEGRVA
                     RSAAVREPSRSRAASARA"
     gene            complement(2301..3161)
                     /locus_tag="VN041_01175"
     CDS             complement(2301..3161)
                     /locus_tag="VN041_01175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWU27692.1"
                     /translation="MSADTERGSVEIIEGDNLEAAGTLASGSFSLVYLDPPFNTGRAQ
                     ERQVVTARRAGDEPEGEVRHGFHGLAYERVRGMLRAYDDRFDDYGDFLVPRLEEAWRL
                     LADDGTLYLHLDYREAHYAKVMLDAVFGRDAFLNELIWAYDYGAKSRRRWPTKHDTIL
                     VYVKTPGGHFFDSDAVDREPYMAPGLVTAEKAARGKLPTDVWWHTIVPTTGREKTGYP
                     TQKPEGVLRRIVQASSRPGDRVLDLFAGSGTTGAVASALGRDAVLVDHNPEAVRVMRE
                     RMPHASVTAL"
     gene            complement(3174..4223)
                     /locus_tag="VN041_01180"
     CDS             complement(3174..4223)
                     /locus_tag="VN041_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HWU27693.1"
                     /translation="MHGEYKVPGGKLVVVDLEVEDGLIRDFRLAGDFFLEPDTALEAI
                     NAAVEGMPVEADVSTIAAAVRAALPDGAQLLGFTPEAVGTVVRRALVTAPGWRDFDWE
                     IVHEKAVSPRMNLALDEVLTSRVGEGRRRPTLRIWEWDESAVVIGSFQSYRNEVDPEG
                     AARHGFDVVRRISGGGAMMMAAGQIITYSLYVPASLVQGMTFADSYAFLDDWVLQALR
                     SVGIDATYQPLNDIASPTGKIGGAAQKRLANGGVLHHATISYDIDGQTMTEVLRIGRE
                     KLSDKGTTSAAKRVDPLRTQTGMERSEIIERFKETFRALTGAEDGAITADEYADAEAL
                     VESKFATDAWLHRVP"
     gene            complement(4247..4945)
                     /gene="pnuC"
                     /locus_tag="VN041_01185"
     CDS             complement(4247..4945)
                     /gene="pnuC"
                     /locus_tag="VN041_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HWU27694.1"
                     /translation="MNEVLHWLLDAFNAQIPVGSSSLLVREVVGNVFGLASALGAMRR
                     RLWAWPVGIIGNALLLTVFLGSVFGTDHTANLFGQAGRQVMFIAVSIYGWVRWKQARD
                     GEGHAIVPRWASGRTRVLLVGALLVGTAGLTPIFRTLGSYEPVWSDAWTFVGSLLATW
                     AMAKGWVEFWLIWVAVDAVGVPLLFSAGYYATAFMYLFYGGFTIVGFFVWMRASRGDK
                     PRIDTGLPDPTVRA"
     gene            complement(4942..5826)
                     /locus_tag="VN041_01190"
     CDS             complement(4942..5826)
                     /locus_tag="VN041_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU27695.1"
                     /translation="MSSESSVAKRTAEFTDAHGIAIVYDVYEAQGEARGVVQLLHGVG
                     EHAGRYEALIAELTAAGFHVYADDHRGHGRTGIRQWNGPARLGHLGKGGMRATVAACW
                     QLTQVIQSERPGLPIVLLGHSWGSFLSQMLFNQHPEAYSAVILSGSAYRTPTDMNPLP
                     LNKQWDGPDADGLEWLSRDSAVWQAFHDDPLTVETPLLKLFGPIEAARMYGRPKKDLG
                     RDVPVLLLVGRDDPVGGPHSVHKLADAYRNRSGLSDVTTLVYADARHEIFNELQQDQV
                     RADVLAWLDKHVPAASTR"
     gene            complement(5899..6312)
                     /locus_tag="VN041_01195"
     CDS             complement(5899..6312)
                     /locus_tag="VN041_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWU27696.1"
                     /translation="MNVNEHELIALGDSDKTLAKEDDIRGRDVKDVGGEDLGKVDDLL
                     VDTAEEKVRFLVVGSGGFLGLGEQKSYIPVDAVTDVTEHEVRIDQSREKLRSAPAYDP
                     ELINDVAYNEDVFGYYGLAPFWSAGYVYPMYPFHR"
     gene            complement(6428..>6723)
                     /locus_tag="VN041_01200"
     CDS             complement(6428..>6723)
                     /locus_tag="VN041_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HWU27697.1"
                     /translation="GGASQAIDGIDTLRALVAEAAGGIEVMAGSGIDVDTVAAIAATG
                     VDAVHFSAKRTVHADGGVRMGSASDGVGGYEVTDRETALAIVAALRAAETSAA"
BASE COUNT         1012 a         2345 c         2374 g          992 t
ORIGIN      
        1 ccgatctcag gctcggccgc atcgccgccc acggcgagca gtgcgccgtc cgcgcatccc
       61 acgaccacgc cgacgaccgt cgtgatggtc cctgccgcgt gcgcgcatgc cagccgcccg
      121 cgctcctccc ccttcgcggt caggatgcgc agctcctgcg ggtcgccgtc ggcatccgca
      181 gcggtgacga gggcgccctc cgcgatcggg acgacgaggc cgtcgtgctc gtcgacgtcg
      241 atgcggaacc gctcgacgat gtcgccgtcg gcgagcgcgg cggtctcgag gagcacggcc
      301 tcgtccgcga agaggatgcc gacgccggtc tcgcccggca ccacggtcgc cgtgccgctc
      361 cccttcacct cgccgacgac gcgcgagggc gcctcgtagt agtggaagtg gtcgatgtga
      421 ctccaggtcc acacaccgct gtcgatgatc gtgaccgcac cgtcgtggat cgtgaacagg
      481 taccttccgt cggtgaccac ctcgacggcg ggttcggttc gggtcagcgc ctcggtggtc
      541 tcgtcgagca ggtcgaggtg cgcgacttcg ccgtccggcg ccacggtggc gagatgcagc
      601 gccggttcgg cgagctcatg ggcgccggcg atcgcgccat gtccgtctac ggtcggaggc
      661 ggcgaggatg tgtcagcgcc gggggcggtg gagcagccgc tcaacgcggc gagcaaggcg
      721 atcgatgcga cggcaatcgg gcggagtctc atgtgttcct ctcggaggga cggatcaggt
      781 ggcgaagcag cgccgacagg ggcgctgcga ggattgcgag gaacgcgacg gtcgatcccg
      841 cagcggtgcc cgcatgccag gagatgagca ggccggcggc gacgctggcg aagccgaccc
      901 cggcggccac cgccatggtc gtgacgatgc gcgacgccca cgcgcccgcg gccacagcgg
      961 ggcccagcag catgccgacc acgaggagcg atccgaccac ctggtacgcg gcgaccacag
     1021 cgagggtgac cattcccacg agcaggatgt gcgcgaggcg cggcctcagg cccagcaatt
     1081 gcgcctgacg aggatccacc gtcagtgcga cgaaggaacg gtggccgacg gcagcgacca
     1141 gcagggtgag tgcggccacg atcgccagct gcacgacgtc gtccacccct gcggcgagga
     1201 tgtcgccgaa gaggatcgcc gtggcatccg tcgcgaagct tcgggaggcg gagatgatga
     1261 tgacccccga ggcgaggctc gcgacgaaga gcaggccgat gctcgtgtcg gccgaaaggc
     1321 gtcctcgtcg ctgcagcgcg gcgacgccga cggccatcgc agaggcactg accccggctc
     1381 cgaggatcgc gggcacgccg atcagtgtgg cggccgcgac acccgggagc attccgtgcg
     1441 cgagggcttc gccgaggaat gccatgccgc ggagcacgac ccaggtgccg accacggcgc
     1501 agaccagcgc gacgagcagg cccacggtca ggccgcgctg cacgaagccg gcggtgaagg
     1561 gttcgatgag gggggacacg ctgaccgact gtagcagtta ttgataacga ttctcatttg
     1621 caataatgag actatgtcgt ctgcaccccg tctcctcgac accacccggg tcctcgcctt
     1681 cgatgacgtc cacgtcatcc gcgacggtca cgccgcgctg cgcggcgtcg acgtcgaggc
     1741 gcacgcgggc cgcctggtgg ccgtcgtcgg caccaacggc tcgggcaagt cgacgttgct
     1801 cgagaccgct gcagggctgc tgcatccgca gcggggcgcg gtgtggagac gcgacggcct
     1861 gcggatgacc ttcgtgccgc agagcacagc gctccccccg cacctgccgc tcacggtcgc
     1921 cgacatcgtg gcgatgggca cctgggcgcg tctcggcctc tggcgccggc cgcagcgggt
     1981 cgatcgagca gccatcgccg aggcgatcgc catggtcggg ctgcgatctc agtcgaaccg
     2041 acgcatcggt gcactgtcgg gaggtcagcg ccagcgcgcc ctgctcgcac aggctctcgt
     2101 gcagcgggcg gacctcgtgc tcctcgacga gccgatggcc ggcctcgacg cagcgacccg
     2161 catggtcgtc ggcggagccg tcgatcgact caccgctgac ggtgcggccg tgatcgtcgt
     2221 gacccacgac ctcgacgagc tcgcccgcgc ggacgaggtc ctcacgctga acgagggtcg
     2281 cgtggcccga tcggcagccg tcagagagcc gtcacgctcg cgtgcggcat ccgctcgcgc
     2341 atgacgcgca ccgcctcggg attgtggtcg acgagcacgg cgtcgcggcc gagggcggat
     2401 gccaccgcgc cggtcgtgcc cgatccggcg aacaggtcga gcacccggtc gcccggtcgt
     2461 gacgacgcct gcacgatgcg gcgcagcacg ccctcgggct tctgggtcgg gtacccggtc
     2521 ttctcgcgtc ccgtggtcgg cacgatcgtg tgccaccaga cgtcggtggg cagcttgccg
     2581 cgggcggcct tctcggccgt gaccaggccc ggtgccatgt agggctcacg gtccacggca
     2641 tccgagtcga agaagtgccc gccgggcgtc ttgacgtaga ccaggatcgt gtcgtgcttg
     2701 gtgggccagc gccgccggga cttggcgccg tagtcgtagg cccagatcag ctcgttcagg
     2761 aacgcgtcgc ggccgaacac cgcatccagc atgaccttgg cgtagtgcgc ctcgcggtag
     2821 tccaggtgca ggtacagcgt gccgtcgtcg gcgagcagcc gccaggcctc ctcgagccgc
     2881 ggcacgagga agtcgccgta gtcgtcgaag cggtcgtcgt aggcgcgcag catcccccgc
     2941 acgcgttcgt aggccaggcc gtggaatccg tgcctgacct cgccctcggg ctcgtcgccc
     3001 gcccggcggg cggtcacgac ctgccgttcc tgcgcacggc cggtgttgaa gggcgggtcg
     3061 aggtagacga gggagaagga tccggatgcc agtgtgccgg ccgcctcgag gttgtcgccc
     3121 tcgatgatct cgaccgagcc gcgctcggtg tcagcgctca ctgcgagccg cgttcacgga
     3181 acacggtgca gccacgcgtc ggtggcgaac ttcgactcga ccagggcctc ggcgtccgca
     3241 tactcatccg ccgtgatcgc accgtcctcg gcgccggtca gcgcgcggaa cgtctccttg
     3301 aagcgctcga tgatctcgct gcgctccatg ccggtctggg tgcgcagcgg atcaacccgc
     3361 ttggcggcgg aggtggtgcc cttgtcgctg agcttctcgc ggccgatgcg cagcacctcg
     3421 gtcatggtct ggccgtcgat gtcgtacgag atggtcgcgt ggtgcagcac gccgccgttc
     3481 gcgagacgct tctgcgcggc gccgccgatc ttgccggtgg gcgaggcgat gtcgttgagg
     3541 ggctggtagg tcgcgtcgat gccgaccgag cgcagcgcct gcagcaccca gtcgtcgagg
     3601 aacgcgtagg agtcggcgaa ggtcatgccc tgcacgaggg atgcaggcac gtacagcgag
     3661 taggtgatga tctggcccgc ggccatcatc atcgcgccac ccccggagat gcggcgcacg
     3721 acgtcgaagc cgtggcgggc cgcgccctcc gggtcgacct cgttgcggta cgactggaac
     3781 gagccgatca ccactgccga ctcgtcccac tcccagatgc gcagcgtggg acggcggcgc
     3841 ccctcgccca cgcgcgaggt gagcacctcg tcgagcgcga ggttcatccg cggcgagacg
     3901 gccttctcgt gcacgatctc ccagtcgaag tcgcgccagc cgggagcggt caccagggcg
     3961 cggcgcacca cggtgccgac cgcctcgggg gtgaagccca gcagctgcgc gccgtcgggc
     4021 agcgcagcgc gcaccgcggc ggcgatcgtg gagacgtcgg cttcgacggg catcccctcg
     4081 acggcggcgt tgatcgcctc cagtgccgtg tccggctcga ggaagaagtc tcccgcgagg
     4141 cggaagtcgc ggatcagtcc gtcttcgacc tcgaggtcga cgacgacgag cttgccaccg
     4201 ggaaccttgt actcaccatg caccgctcca gcttaacggc gcgctctcag gcgcggacgg
     4261 tcgggtcggg caggccggtg tcgatgcggg gcttgtcacc gcgggacgcc ctcatccaca
     4321 cgaagaagcc cacgatggtg aagccgccgt agaacaggta catgaacgcg gtcgcgtagt
     4381 agccggccga gaacagcagc ggcacgccca cagcatccac cgccacccag atcagccaga
     4441 actcgaccca ccccttggcc atcgcccagg tcgccagcag cgatccgacg aaggtccagg
     4501 cgtcgctcca caccggctcg tacgagccca gcgtccggaa gatcggggtc aggcccgccg
     4561 tgccgacgag cagcgcgccg accagcagca cgcgcgtgcg cccggaggcc cagcgcggca
     4621 cgatcgcgtg cccctcgccg tctctcgcct gcttccagcg cacccagccg tagatcgaga
     4681 cggcgatgaa catcacctgc cggccggcct ggccgaacag gttcgcggtg tgatcggtgc
     4741 cgaacacgct gcccaggaac accgtcagca gcagggcgtt gccgatgatc cccacgggcc
     4801 aggcccacag ccggcggcgc atcgcgccga gagcggatgc cagtccgaag acgtttccga
     4861 ccacctcgcg caccaggagc gacgacgatc cgacggggat ctgcgcgttg aaggcgtcca
     4921 gcagccagtg caggacctcg ttcaccgagt gctcgcggcg gggacgtgct tgtcgagcca
     4981 ggcgagcacg tcggcgcgca cctgatcctg ctgcagctcg ttgaagatct cgtgccgggc
     5041 gtcggcgtag acgagcgtgg tcacgtcgct caggccggag cggttccggt acgcgtccgc
     5101 gagcttgtgc acgctgtgcg gcccgccgac cgggtcgtca cggcccacga gcagcagcac
     5161 tggcacgtcc ctgccgaggt ccttcttcgg ccgtccgtac atgcgcgccg cctcgatcgg
     5221 gccgaagagc ttcagcagcg gggtctcgac ggtgagggga tcgtcgtgga aggcctgcca
     5281 cacggccgag tcgcgcgaga gccactccag gccgtcggcg tccggcccgt cccactgctt
     5341 gttcagtggc agcgggttca tgtcggtcgg ggtgcggtac gcggagccgg agaggatcac
     5401 agccgaatag gcctccggat gctggttgaa cagcatctgc gagaggaagg acccccagga
     5461 gtgcccgagc agcacgatcg ggagcccggg acgctcgctc tggatgacct gcgtgagctg
     5521 ccagcatgcc gcgacggtcg cccgcatgcc gcccttgccg aggtgaccga gcctggcggg
     5581 gccgttccac tgccggatgc cggtgcgccc gtgcccgcgg tggtcgtcgg cgtacacgtg
     5641 gaagccggcg gctgtgagct cggcgatgag cgcctcgtac cggcccgcgt gctcgccgac
     5701 gccgtgcagc agctgcacga ctcctctcgc ctcgccctgc gcctcgtaca cgtcgtagac
     5761 gatcgcgatc ccgtgggcgt cggtgaactc cgctgttctc ttggcgacgc tgctctcaga
     5821 agacatgccc cgagtctacg gatgcccggc ggccggcatc cgctcgtttc gccgagcgac
     5881 gccgcaggcc gcgagcgctc agcggtggaa cgggtacatc ggatagacgt agccggccga
     5941 ccagaacggc gcgaggccgt agtagccgaa cacgtcctcg ttgtacgcga cgtcgttgat
     6001 cagctcgggg tcgtaggcgg gggcggaacg cagcttctcc cggctctggt cgatgcgcac
     6061 ctcgtgctcg gtgacgtcgg tcacagcgtc caccgggatg taggacttct gctcgcccag
     6121 ccccaggaac ccgcccgatc cgacgaccag gaagcgcacc ttctcctcgg cggtgtcgac
     6181 gaggaggtcg tcgacctttc cgaggtcctc gcccccgacg tccttgacgt cgcggccgcg
     6241 gatgtcgtct tccttcgcga gggtcttgtc gctgtcgccg agggcgatca gctcgtgctc
     6301 gttgacgttc atggcactct ccttccaggt cgtgaaacct tctcaggaga tgccgcgtca
     6361 gggcaggggc tggcctcgcc cgagcaggcg gagtagcctc agcggtgcgg cgtgcggtcg
     6421 cgcggtgtca ggccgcggac gtctccgcgg cgcgcagagc ggcgacgatg gcgagggccg
     6481 tctcgcggtc ggtgacctcg tatccgccga caccgtcact ggccgatccc atccgcacac
     6541 ccccgtcggc gtgaaccgtg cgcttggccg agaagtgcac ggcgtcgacc ccggtcgcgg
     6601 cgatggcggc gacggtgtcg acatcgatgc cgctgcccgc catgacctcg atgccgccgg
     6661 cggcctcggc gaccagcgcc cgcagtgtgt cgatcccgtc aatggcctgc gaggcgccgc
     6721 cgg
//
LOCUS       DATMSX010000036         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_171075,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000036 DATMSX010000000
VERSION     DATMSX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171075"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN041_01205"
     CDS             <1..217
                     /locus_tag="VN041_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HWU27698.1"
                     /translation="SAADLAARSPAARAGIAAGDWVLWRVVPERIEFWQGAQDRDHLR
                     LVYRRGEHGWTRAVERAESGSEEKEKR"
     gene            214..642
                     /locus_tag="VN041_01210"
     CDS             214..642
                     /locus_tag="VN041_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU27699.1"
                     /translation="MSDYQVTEIGALQDWRAFHGGFDEARSRDGRRVVDHELTMQYIG
                     MTANALVPGEEAGYWHVHEKVEELYVFLAGRGQMGLDDDVVDVEEGTVVRVGQGVWRT
                     WRALPDSPGELRWLCIRAGGEHLPHMPDDSRRDPDRPMPW"
     gene            731..1723
                     /locus_tag="VN041_01215"
     CDS             731..1723
                     /locus_tag="VN041_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWU27700.1"
                     /translation="MTQVLVLGGTGWLSGRIAQRWRDAGARVTCLARGGRPAPEGTVL
                     LPGDRDDPAVYDRLSEQHWDEIVDISSRARHVREAVAALGASTAHWTYVSSMSAYSDD
                     TTVGLDEAGALHEPAQEGDEYEYGAEKVAAERAVAVLGERAFIVRPGLIVGEGDPSDR
                     FGYWAAAFDRAGNRPVLVPTLADRYTQVIDVDDVAVLLVGGSRHGIVNAIGDALPLVD
                     VLSRIRSATGHTGELLEASDKWLREHGVDYWMGERSLPLWLPADMPGFARRDNAAYKA
                     SGGRLTPIDDTIRRVLQDERVRGVDRERRAGLTRTEEQELIDQLVRADADPADA"
     regulatory      1805..1898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1890..>2159
                     /locus_tag="VN041_01220"
     CDS             1890..>2159
                     /locus_tag="VN041_01220"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HWU27701.1"
                     /translation="MSDPLRPASAPGLVTEAADLLGALRAAPPLTQCITNAVVTGFTA
                     NVLLAVGAAPAMVDIVGEAGMFAGISSGVLINLGTPTPEQRAAGLE"
BASE COUNT          314 a          724 c          818 g          303 t
ORIGIN      
        1 gtcggcggct gatctcgccg ctcgttcgcc cgcggcccgt gccgggatcg cggccggcga
       61 ctgggtgctc tggcgcgtcg tgccggagcg catcgagttc tggcagggcg cgcaggatcg
      121 tgaccatctg cggctggtgt accgccgggg cgagcacggc tggacccgcg cggtcgagcg
      181 ggcggagagc ggatcggaag agaaggagaa gcgatgagcg actaccaggt gaccgagatc
      241 ggtgcgctgc aggactggcg tgcgttccac ggcggtttcg acgaggcgcg ctcgcgcgac
      301 ggccgccgcg tcgtcgacca cgagctgacc atgcagtaca tcggcatgac ggcgaacgcc
      361 ctggtcccgg gtgaggaagc cgggtactgg cacgtgcatg agaaggtcga ggagctctac
      421 gtcttcctcg ccgggcgcgg ccagatgggc ctggacgacg acgtcgtcga cgtggaggag
      481 ggcacggtcg tgcgcgtggg tcagggcgtg tggcgcacct ggcgggcact gccggacagc
      541 ccgggcgagc tgcgctggct ctgcatccgg gccggcggtg agcaccttcc gcacatgccc
      601 gacgacagcc gtcgcgaccc ggaccgtccg atgccgtggt gaatactcgc gtcggtcccc
      661 cgaagaccga gatgctctgc tgagggcgga acctcttcct gcacggcgcg gagactgctg
      721 tcatggtcgt atgacacagg tgctggtgct cggtggaaca gggtggctct cggggcggat
      781 cgcgcagaga tggcgggatg ccggcgcccg ggtgacctgc ctcgcgcggg gcggacgacc
      841 ggcgccggag ggcacggtgc tgctgcccgg ggatcgcgat gatccggcgg tgtacgaccg
      901 cctgagcgag cagcactggg acgagatcgt cgacatctcc tcccgggcac ggcacgtgcg
      961 cgaggcggtc gcggcgctcg gcgcgagcac ggcgcactgg acctacgtct cgtcgatgtc
     1021 ggcgtactcc gacgacacga ccgtcgggct cgacgaagcc ggggcactgc acgagcccgc
     1081 gcaggagggc gacgagtacg agtacggcgc ggagaaggtc gccgcggagc gcgccgtggc
     1141 ggtgctgggg gagcgggcgt tcatcgtccg gcccggcctg atcgtcggcg aaggggatcc
     1201 cagcgaccgc ttcgggtact gggcggcggc gttcgaccgt gcggggaacc ggcccgtgct
     1261 cgtccccacc ctcgccgaca ggtacacgca ggtgatcgac gtcgacgacg tcgccgtgct
     1321 gctggtgggc gggagcaggc acgggatcgt caacgcgatc ggcgacgccc tgccgctcgt
     1381 cgacgtcctg agccgcatcc gctccgccac cgggcacacc ggggagctgc tcgaggcctc
     1441 cgacaagtgg ctgcgcgagc acggggtgga ctactggatg ggggagcgct ccctgccgct
     1501 gtggctcccc gccgacatgc cggggttcgc gcgtcgggac aacgctgcgt acaaggcatc
     1561 cggtggccgt ctcacgccga tcgacgacac gatccggcgc gttctccagg atgagcgcgt
     1621 ccgcggtgtg gatcgcgagc gccgcgcggg actgacccgc acggaggagc aggagctcat
     1681 cgatcagctc gtgagggccg acgccgatcc ggctgacgcc tgagacgatc ggacgcccgc
     1741 ccgctgagga cggacgccgg cgtcaccggg cgctccgccg gtgacgccct tcgctagact
     1801 cacgaacagt gacacggggt gccgcatccg cggctgagaa cagacccgtc gaacctgatc
     1861 tagttcgtac tagcgaaggg atgtcgcgaa tgagcgatcc tctgcgtcct gcatccgccc
     1921 ccggcctcgt caccgaggcg gccgacctgc tgggggcgct gcgcgcggcc ccgcccctca
     1981 cgcagtgcat cacgaacgcg gtggtcaccg gcttcaccgc caacgtgctg ctcgccgtcg
     2041 gcgcggcccc ggcgatggtc gacatcgtcg gcgaggccgg catgttcgcg ggcatctcct
     2101 ccggcgtgct gatcaacctc ggcacgccga cgcctgagca gcgcgcggcc ggcctcgaa
//
LOCUS       DATMSX010000037         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_548, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000037 DATMSX010000000
VERSION     DATMSX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VN041_01225"
     CDS             complement(<1..395)
                     /locus_tag="VN041_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HWU27702.1"
                     /translation="MTPFFLLAAAVLLVAFGGLMAAVDAAFSVTSSNDIEELASEGRG
                     SAALHSIAADPDPHVNAVAFMRVLSETTAAVLVTVALDSLLPSIWWAMLAAAVLMTGI
                     TFVLVGASPRSYGRLHADTILRAWAPTVRG"
     gene            complement(392..850)
                     /gene="ybeY"
                     /locus_tag="VN041_01230"
     CDS             complement(392..850)
                     /gene="ybeY"
                     /locus_tag="VN041_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWU27703.1"
                     /translation="MIEINNESAIEIDETVLQRLTDFNLEQLHVSADAEVAIVLVDEG
                     AMEALHVQWMDEPGPTDVLSFPMDELRPGTADRPTPAGLLGDIVLCPQVAETQAQAAG
                     HSLMDELILLTTHGLLHLLGFDHAEPEEEREMFGLQKSLIEGFAHAERRR"
     gene            complement(847..1872)
                     /locus_tag="VN041_01235"
     CDS             complement(847..1872)
                     /locus_tag="VN041_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWU27704.1"
                     /translation="MVQLLGPQDRLLRMLEKQHPRVQVLVRGNEINLGGSAADVAAAK
                     DLVEELLEMTRSGYDLVPSDVEHSARMLRREDGPRPSEVLGEAILSTRGRVIRPKTLG
                     QKEYVDAIEENTIVFGIGPAGTGKTYLAMAKAVQALQRKEVERIILTRPAVEAGERLG
                     FLPGSLTDKIDPYLRPLYDALNEMMDPEVVPRLMATGTIEVAPLAYMRGRTLNSSFVV
                     LDEAQNTTPEQMKMFLTRLGFGTKMVVTGDITQIDLPQGASGLRLVTRVLDGIDDIHF
                     SRLTSDDVVRHSLVGRIVDAYSEYDERRTADRVEREQAHQFANRADRRVGLRPNLRDR
                     KPKRGLE"
     gene            complement(1958..2305)
                     /locus_tag="VN041_01240"
     CDS             complement(1958..2305)
                     /locus_tag="VN041_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWU27705.1"
                     /translation="MTEPSIFTRILNGEIPGEFVAQTDHLFAIRDINPQAPVHVLVIP
                     KTEQYRDVTELAAGDPGLMAEMVAMAKQIAVELANGEYRLIFNNGASAAQSVFHVHAH
                     VLAGIEENKLVGF"
     gene            complement(2320..>2612)
                     /locus_tag="VN041_01245"
     CDS             complement(2320..>2612)
                     /locus_tag="VN041_01245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWU27706.1"
                     /translation="IPEVAEVESTARLAARAADARMLVLDPWTDTRLTGIRPDGRDIV
                     LVVGPEGGIAPEELERLEAAGAERVRLGDTVLRTSTAGPVAIALLSANLGRW"
BASE COUNT          378 a          895 c          918 g          421 t
ORIGIN      
        1 ccgcgcaccg tcggcgccca ggcccgcagg atggtgtcgg cgtgcagccg cccgtaggag
       61 cgcgggctgg cgccgaccag cacgaaggtg atccccgtca tcagcaccgc ggcggcgagc
      121 atggcccacc agatgctcgg cagcagcgaa tccagcgcca ccgtcaccag caccgcggcg
      181 gtcgtctcgg acagcacgcg catgaacgcg accgcgttca cgtgcgggtc ggggtcggcg
      241 gcgatgctgt gcagggcggc ggatccgcgc ccctcggagg cgagctcctc gatgtcattg
      301 ctcgaggtca ccgagaacgc ggcgtccacc gccgccatca ggccgccgaa cgcgaccagc
      361 agcacagctg ccgcaagcag gaagaacggg gtcatcgacg gcgttcggcg tgcgcgaatc
      421 cctcgatcag agacttctgc agtccgaaca tctcacgctc ctcttcaggc tcggcgtggt
      481 cgaagcccag caggtgcagc agcccgtgcg tggtgagcag gatcagctcg tccatcaggg
      541 agtgcccggc ggcctgcgcc tgcgtctccg cgacctgcgg gcacagcacg atgtcgccca
      601 gcagccccgc gggggtcggc cggtcggcgg tgcccggtcg cagctcgtcc atcgggaagc
      661 tcagcacgtc ggtcggcccc ggctcgtcca tccactgcac gtgcagcgcc tccatggcgc
      721 cctcgtcgac gagcacgatc gcgacctcgg catccgcgct gacatgcagc tgctcaaggt
      781 tgaagtcggt gagtcgctgc agcaccgtct cgtcgatctc gatggccgac tcgttgttga
      841 tctcgatcat tccaggcctc gtttcggctt acggtctcgg aggttcgggc gcaggcccac
      901 tcggcggtcg gcgcggttcg cgaactggtg cgcctgctcg cgctccaccc ggtcggcggt
      961 gcggcgctcg tcgtactcgc tgtacgcgtc gacgatgcgg cccaccagag aatgacgcac
     1021 gacgtcgtcg ctggtcagcc gggagaagtg gatgtcatcg atcccgtcga gcacgcgggt
     1081 caccaggcgc aggccggacg cgccctgcgg caggtcgatc tgcgtgatgt cgcccgtgac
     1141 caccatcttg gtgccgaacc cgagccgcgt caggaacatc ttcatctgct cgggggtggt
     1201 gttctgcgcc tcgtccagca ccacgaacga gctgttcagc gtacgtccgc gcatgtaggc
     1261 gaggggtgcg acctcgatgg tgccggtcgc catcaggcgg ggcacgacct cggggtccat
     1321 catctcgttc agggcgtcgt acagcggccg caggtagggg tcgatcttgt cggtgagcga
     1381 accgggcagg aatccgagcc gctcgcccgc ctccacggcg gggcgggtga ggatgatgcg
     1441 ctcgacctct ttgcgctgca gcgcctgcac ggccttggcc atcgcgaggt aggtcttgcc
     1501 ggttccggcg ggaccgatgc cgaagacgat cgtgttctcc tcgatcgcgt cgacgtactc
     1561 cttctgcccg agcgtcttcg ggcggatcac ccggccgcgc gtggacagga tggcctcgcc
     1621 gagcacctcg ctcggacgcg gcccgtcctc gcgacgcagc atccgggcgg agtgctccac
     1681 gtcgctgggg acgaggtcgt acccggagcg ggtcatctcc agcagctcct cgaccaggtc
     1741 cttggccgcg gcgacgtcgg cagccgagcc gccgaggttg atctcgttgc cgcgcacgag
     1801 cacctggacg cgcggatgct gcttctccag catccgaagc aggcggtcct gcgggccgag
     1861 cagctgcacc atggcgacgc cgtcggcgta gatgcgctcg gtgcgctccg gctgagcggg
     1921 gaactgcggt gcggagtcgt caggggacga cgcagtgtca gaagccaacg agcttgttct
     1981 cctcgatacc tgcgagcacg tgcgcgtgca cgtggaagac ggactgggcg gcgctggcgc
     2041 cgttgttgaa gatcagacgg tactcgccgt tggccagctc cacggcgatc tgcttggcca
     2101 tggcgaccat ctcggccatc aggcccgggt cgcccgcggc gagctcggtg acgtcgcggt
     2161 actgctcggt cttggggatg acgagcacgt gcacgggtgc ctgcgggttg atgtcgcgga
     2221 tggcgaagag gtgatccgtc tgggcgacga actcgccggg gatctccccg ttcaggatgc
     2281 gggtgaagat cgagggctct gtcatgcggt caagtctagt taccagcgtc ccaggttggc
     2341 cgagagcagc gcgatcgcga ccggcccggc cgtggaggtg cgcagcaccg tgtcacccag
     2401 ccgcacgcgc tcggcgccgg cggcctcgag ccgttccagc tcctccgggg cgatcccgcc
     2461 ctcgggcccg accaccagca cgatgtcacg accgtcgggc cggatgccgg tgagccgcgt
     2521 gtcggtccag gggtcgagca caagcatcct ggcgtccgcg gcgcgcgccg cgagcctcgc
     2581 ggtgctctcg acctcggcga cctccgggat cc
//
LOCUS       DATMSX010000038         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_104788,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000038 DATMSX010000000
VERSION     DATMSX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104788"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2272)
                     /locus_tag="VN041_01250"
     CDS             complement(53..2272)
                     /locus_tag="VN041_01250"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWU27707.1"
                     /translation="MTDDAIIYTYTDEAPALATASFLPIIKAYTGQAGIEVETRDISL
                     AGRILAAFPQKLTPEQQVGDALAELGGLATLPEANIIKLPNISASIPQLKAAIAELQE
                     KGFDVPDYPDEPQSLEERDVRARYDKIKGSAVNPVLREGNSDRRAPLAVKNYAKKHPH
                     RNKPFAEGSKTRVATMGHDDFKHNERSWVAAHDDVLSFQHIAADGTITVLKEGLKVLP
                     REIIDATFLSAADLDAFLAETLEQAKAEGVLYSVHLKATMMKVSDPIIFGHVVKAFFK
                     DVFAQYGDKLAEAGLSANNGLGSILSGLADIAGGDEIAAAFDKAIAEGPRLSYVNSDK
                     GITNLHVPSDVIVDASMPALVRNGGKLWGKDGGEADTIAVIPDSSYAGVYQAVIDDVI
                     ANGPLDPATIGTVPNVGLMAQAAEEYGSHDKTFEIASAGVVQILDSDGTVLISHEVGA
                     GDIWRATQTKHIPVMDWVKLAVTRARATGAPAVFWLDANRSHDAQIIAKVHQGLATLD
                     TKGLTITILAPEDATRYTLARLRKGEDTISVTGNVLRDYLTDLFPILEVGTSAKMLSI
                     VPLLAGGGLFETGAGGSAPKHVQQLVEEDYLRWDSLGEFFALAASLEHFADRTGNEKA
                     RVLAETLDAATGTFLEQDRSPGRALGTIDNRGSHFYLGLYWAQELAKQTKDAELAAAF
                     APVAATLAENEQKIVDELNAVQGKHADIGGYYRPDVAKVAAVMRPSATLNAVIDALV"
     gene            2410..2868
                     /locus_tag="VN041_01255"
     CDS             2410..2868
                     /locus_tag="VN041_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27708.1"
                     /translation="MSELRVVELSAATIVAVNNLSLKPGQEQFLAPVSYGIAATVVNP
                     QTSWQRVVLDGDEVVGFVSASFDADAPEEHFRSVLWRINVDADDQGRGVGRFAVKTLI
                     DEARARGVDHVNVIYEAGEGGPEAFFRRVGFEPVGETEYSEVIAQIRVTD"
     gene            3158..4021
                     /locus_tag="VN041_01260"
     CDS             3158..4021
                     /locus_tag="VN041_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWU27709.1"
                     /translation="MDLRSWLRSRNGIAHRDDAADAGFTVFRIRSAIRSGAVRRIRSR
                     WIALESAPIDLADAAESGGAVTCVSLARRRGWWVPPDTDECLHLRMPPNGVVRVSDAH
                     AHWGRSLAPPRPRMLETSVEDALSDAANCFPVDQAVAIWESAVRTESISLDSLRTVRW
                     RTPAARRCLELLRPGTDSSLETVFHVRLSPWGVRLRFQVQLAGHPVDFLIGTHLVIQV
                     DGWSFHSSSADRSRDLAHDAELRMRGYTVLRFSYAQIIYDWEHIERTLAGAVSRGLHV
                     APVTSGGRPRF"
     gene            complement(4122..>4559)
                     /locus_tag="VN041_01265"
     CDS             complement(4122..>4559)
                     /locus_tag="VN041_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27710.1"
                     /translation="ERSSLASAADLPLDRLRARHDAVLARWMAYETDPGLALAYPAMS
                     DGRQPHTAAFLAAVERARDRRPPSEGARTTASDFSDYRRAVDDLERAFDVAERSARGE
                     KVQPDLPDALWDAARGFAEKSTEMLNRTSDLLGDWTARRRRDR"
BASE COUNT          636 a         1518 c         1616 g          789 t
ORIGIN      
        1 agacgaggaa cgggcggatg ctgtgcagca tccgcccgtt ctgaggtcct gatcagacca
       61 gcgcgtcgat gacggcgttg agcgtggccg agggacgcat gaccgctgcg accttggcga
      121 cgtcggggcg gtagtagccg ccgatgtcgg cgtgcttgcc ctgcacggcg ttcagctcgt
      181 cgacgatctt ctgctcgttc tcggccagcg tcgccgcgac gggcgcgaac gcggcagcca
      241 gctcggcgtc cttcgtctgc ttggccagct cctgcgccca gtacaggccc aggtagaagt
      301 ggctgccgcg gttgtcgatg gtgcccagcg cgcgaccggg cgagcggtcc tgctcgagga
      361 aggtgccggt cgcggcatcc agggtctcgg ccagcacgcg ggccttctcg ttgcccgtac
      421 ggtccgcgaa gtgctcgagc gacgccgcca gcgcgaagaa ctcgcccagc gagtcccagc
      481 gcaggtagtc ctcctccacc agctgctgca cgtgcttcgg cgcggagccg cccgcaccgg
      541 tctcgaacag gccgccgccg gcgagcagcg ggacgatcga gagcatcttg gcgctggtgc
      601 cgacctcgag gatcgggaac aggtccgtca ggtagtcgcg cagcacgttg ccggtgaccg
      661 agatcgtgtc ctcgcccttg cgcaggcgcg ccagcgtgta gcgggtggcg tcctcgggcg
      721 cgaggatcgt gatcgtcagg cccttggtgt cgagggtcgc gagaccctgg tgcaccttgg
      781 cgatgatctg ggcgtcgtgc gagcggttgg cgtccagcca gaacaccgcg ggagcaccgg
      841 tggcgcgggc acgggtcacg gcgagcttga cccagtccat cacggggatg tgcttggtct
      901 gcgtggcgcg ccagatgtcg ccggcgccga cctcgtgcga gatcagcacg gtgccgtcgc
      961 tgtcgaggat ctgcacgaca cccgccgagg cgatctcgaa ggtcttgtcg tggctgccgt
     1021 actcctcggc ggcctgggcc atcaggccca cgttcggcac ggtgccgatc gtggcggggt
     1081 ccagcgggcc gttggcgatg acgtcgtcga tcacggcctg gtacacgccc gcgtacgacg
     1141 agtccgggat gaccgcgatg gtgtcggcct cgccgccgtc cttgccccac agcttgccgc
     1201 cgttgcggac cagggcgggc atggaggcgt cgacgatcac gtcgctcggg acgtgcaggt
     1261 tggtgatgcc cttgtcggag ttcacgtacg acagacgcgg cccctcggcg atggccttgt
     1321 cgaacgccgc agcgatctcg tcgccgcccg cgatgtcggc gagaccggag aggatcgatc
     1381 cgaggccgtt gttggcgctc aggccggcct cggcgagctt gtcgccgtac tgcgcgaaca
     1441 cgtccttgaa gaacgccttc acgacgtgac cgaagatgat cgggtcgctg accttcatca
     1501 tggtggcctt caggtgcacc gagtacagca cgccctcggc cttggcctgc tcgagcgtct
     1561 ccgcgaggaa ggcgtccagg tcggcggcgg agaggaacgt cgcgtcgatg atctcgcgcg
     1621 gcaggacctt cagcccctcc ttgagcacgg tgatggtgcc gtcggcggcg atgtgctgga
     1681 agctgagcac gtcgtcgtgg gctgcgaccc aggagcgctc gttgtgcttg aagtcgtcgt
     1741 ggcccatggt cgcgacgcgc gtcttggagc cctccgcgaa cggcttgttg cggtgcgggt
     1801 gcttcttggc gtagttcttc acggccaggg gcgcgcggcg gtcgctgttg ccctcgcgga
     1861 gcacggggtt gaccgccgag cccttgatct tgtcgtagcg ggcgcggacg tcgcgctctt
     1921 caagagactg gggctcgtcc gggtagtccg ggacgtcgaa gcccttctcc tgcagctcgg
     1981 cgatggccgc cttcagctgc gggatggacg ccgagatgtt cggcagcttg atgatgttgg
     2041 cctcggggag cgtggccagt ccgcccagct cggcgagggc gtcgcccacc tgctgctcgg
     2101 gggtgagctt ctgggggaag gcggcgagga tgcggccggc gagcgagatg tcgcgggtct
     2161 ccacctcgat gccggcctga ccggtgtagg ccttgatgat cggcaggaag gacgcggtcg
     2221 ccagagccgg cgcctcgtcc gtgtaggtgt agatgatggc gtcgtcggtc accggatctc
     2281 cgttcgcgag ggcagatttt atctcgatac caagataccc gagtccgctt cccggcaccc
     2341 cttcagggtt tcgacaccgg ccctgccgtt tccgcgacag aagcccgcgg aggggctagc
     2401 ctgggttcca tgtcggaact gcgtgtggtc gaactgtccg cggcgacgat cgtcgccgtc
     2461 aacaatctgt ccctcaagcc cggccaggag cagttcctgg caccggtctc ctacgggatc
     2521 gccgccactg tggtgaaccc ccagacctcc tggcagcgcg tagtgctcga cggtgacgag
     2581 gtggtgggct tcgtcagcgc gagcttcgac gcggatgccc ccgaggagca cttccgcagc
     2641 gtgctgtggc gcatcaacgt ggacgccgac gaccagggtc gtggtgtcgg gcgcttcgcc
     2701 gtcaagactc tgatcgacga ggcccgcgca cgcggcgtcg atcacgtgaa cgtgatctac
     2761 gaggccggtg agggcggccc cgaggcgttc ttccgccgcg tgggcttcga gccggtcggc
     2821 gagaccgagt actccgaggt catcgcgcag atccgcgtca cggactgaac cggcggcggg
     2881 gcctcggatc ccctccccca ccgagacccc gtcgccgctc cccctgagag cgctcccgcg
     2941 cggcatccgc ctgctgcgcc gcacgcttgc gcccgtcagt tctgaaggac gcttccactt
     3001 ctgaaggatg ccggcgttgc aggcatcctt cagacctgtg ttcatccttc agaagcctcc
     3061 cgaccactgc gcctgcacat tcgccctccc gggtgggttg tgcacgattc gcgggttccc
     3121 gccccgcgat gacgcggtat tccgccatcg tgggcgcatg gatctcaggt catggctgag
     3181 gagccggaac ggcatcgctc atcgcgatga cgccgctgac gccggcttca cggtcttccg
     3241 tatccgcagc gcgattcgct ccggtgcggt ccgccgcatc cgctcgcgct ggatcgcgct
     3301 cgaatcggct ccgatcgacc tcgccgacgc cgcggagtcg ggcggcgcag tcacctgcgt
     3361 atcgctcgca cgtcgacgtg gatggtgggt gccgccggat accgatgaat gcctgcatct
     3421 ccggatgccc ccgaacggag tggtgcgcgt ctccgacgcc catgcgcact ggggcaggtc
     3481 tctcgcaccc ccacgccctc gcatgctgga gacgtccgtg gaggatgcgc tctcagatgc
     3541 tgcgaactgc ttcccagtgg atcaggcggt cgcgatctgg gagtcggccg tccgcaccga
     3601 atcgatctcc ctcgactcac tgcgcacggt ccgctggcgg actccggcag ctcggcggtg
     3661 cctggaactc ctgcgcccgg gtacggattc gtcactggag acggtgttcc acgtccgact
     3721 gagcccgtgg ggcgtgcggc tccgcttcca ggtgcagctg gcagggcatc cggtcgactt
     3781 cctgatcggc acgcacctgg tcatccaggt cgacggctgg tcgttccact cgtcatccgc
     3841 cgaccgcagc cgcgacctcg cgcacgatgc cgagctgcgc atgaggggct atacggtgct
     3901 gcgcttctcc tacgcgcaga tcatctacga ctgggagcac atcgagcgca cgctcgccgg
     3961 ggccgtcagc cgtgggctgc acgtggcgcc agtcacttct ggaggacgtc ctcgcttctg
     4021 aaggacgcca gggctgcagg catccttcag aagcgtgaat ctccttcaga acgacgcgcg
     4081 acggacggag tatgcgggcg atgagcaggc ggatgccggg ctcagcggtc gcgccggcgc
     4141 cgggcggtcc agtcgccgag caggtcggag gtgcggttca gcatctcggt ggacttctcc
     4201 gcgaacccgc gggcggcatc ccagagggca tccggcaggt cgggctgcac cttctcgccg
     4261 cgtgcggagc gctcggcgac gtcgaaggcc cgctccagat cgtcgacggc gcgacggtag
     4321 tcggagaagt cggatgctgt ggtgcgcgcg ccctcggacg gcggccgccg atcgcgcgcc
     4381 cgttcgacgg cggcgaggaa ggcggccgtg tgcggctgac gtccgtcact catcgccggg
     4441 tacgccagcg ccaggccggg atcggtctcg taggccatcc accgggcgag gaccgcgtcg
     4501 tggcgggcgc gcagccggtc cagggggaga tcagccgcgg aggccagcga cgagcgctc
//
LOCUS       DATMSX010000039         5349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_38495,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000039 DATMSX010000000
VERSION     DATMSX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38495"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VN041_01270"
     CDS             complement(<1..1818)
                     /locus_tag="VN041_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27711.1"
                     /translation="MTEINRRTFLTVGGVGLATLLVGNASTAANAATGRAAAASAKGA
                     PVPASGGTPPGPYTFGWTADDLLDFDPASTPWGRHLQCLIPRAKRIAPFAATQAHPDL
                     DPAVQLSTLTIDDAGSIYEGHNQAIGLEAQVYTQRYWAYIDIWGTWHGQVDGPAPIEM
                     VDGERVPGRPYGVIDIPNPGWTEAAHKNGALSIGGWFWPRPVDFDAFLVQRADGSFPV
                     ADKLIEIRKYFGFDGLFINQEATVTTAQITKFKDFLRYLKRTDPDFYLQGYDSADFDR
                     GFVTYENRLSENNLRWLGTPDAPMYDSIFMNYWWQATTGGADRDLTKSAASAREAGYD
                     PRLVGFAGVEHQKGGFRPEEDFGSVAGPGKPAPTSVALFVDSQIWLDGSWRGETSSPE
                     GRSTYRDLEQRFWSGPTGNPVTSGRLEPRTPPYRTDTLNYRQWDGIAHWITERSPYGA
                     LPIVTDFNVGVGSSFHLDGVQVRTDGWDNMGCADRALTWQYWTEGDLSVTLDESTSFG
                     SAHCLRLRGDGVVHLFKTDLTAAAAMAVSVRAQQLTSVEIGITWKDAPGAIDWRPLEN
                     AGGGTWTEWRGEVDVNARRIARISLRTGGDGRLGRVSLRE"
     gene            2051..2731
                     /locus_tag="VN041_01275"
     CDS             2051..2731
                     /locus_tag="VN041_01275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU27712.1"
                     /translation="MFRPSRDRWLMLLCAALSGAVLTGCAAATAATGSRGAESQRNPV
                     IVTIGDSIMSGHGLDDGEDWPSMLSQRTGVSIDNLACSGAGFLAVGTCGQDFRGLVDA
                     AVKDDPTLVIVQGSDNDVGRSDADITAATKRTVDAIHRAMPRAELVGLSTLWDPSEPK
                     PAEIDVSSAAVRAAVTALGGRYIDIGQPLRGDPSLLQDDDEHPTVAGQQRLVHVIRVE
                     LEHAGYRL"
     gene            complement(2742..4292)
                     /gene="ffh"
                     /locus_tag="VN041_01280"
     CDS             complement(2742..4292)
                     /gene="ffh"
                     /locus_tag="VN041_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWU27713.1"
                     /translation="MATFGTLSDRLTETFRNLRTKGKLSPADVDGTVREIRRALLDAD
                     VALVVVKDFTAKVRERALSDEVSKALNPAQQVVQIVNEELVAILGGEQRRLEFAKTPP
                     TVIMLAGLQGSGKTTFAGKLAKQLEAEGHTPLLVAADLQRPNAVTQLQVVAERAGAAI
                     FAPEPGNGVGDPVQVSKAGVEHARRQQHDVVIIDTAGRLGVDAELMKQAADIRKATDP
                     DEVLFVIDAMIGQDAVNTAKAFQEGVDFTGVVLSKLDGDARGGAALSVASVTGRPIIF
                     ASTGENLEDLEPFHPDRMASRILDLGDILTLIEQAQQAFDEEEAMKMAAKLANEAFTL
                     EDFLEQMQQMRKMGSMKKMLGMLPGMGQMKQQLEDFDEREVDRTEAIIRSMTPAERRN
                     TKILNGSRRLRIARGSGMTVTDVNQLVQRFEQAAKMMKTVARGGTPQIPGMGPMGKPG
                     ASAKRGKKGAKNKGGSRSGNPAKRAAENAGIAMSNSTPTGSGFGLGGQKAPTEADMAE
                     IQKLFGKN"
     gene            complement(4306..4806)
                     /locus_tag="VN041_01285"
     CDS             complement(4306..4806)
                     /locus_tag="VN041_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWU27714.1"
                     /translation="MTSRDEPLTRALESSASDLLAYLERRVGADDAPDLLGETMVVAW
                     RRVKELPPDSERARMWLFGIARGTLLNHSRGQRRRWALADRIRLQVRESATAPPADEG
                     VEVRDAIDRLEPDLAELVRLVHWERLSVADAADLLGIPASTARGRYQRAKDRLRAALR
                     QEAPLP"
     gene            complement(4803..>5349)
                     /locus_tag="VN041_01290"
     CDS             complement(4803..>5349)
                     /locus_tag="VN041_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27715.1"
                     /translation="GTVQTGKTVGVLGGKTPGVGGGVVGVAGAGGAGIATANSDWLWI
                     QGLENPDRSYTYTSPTWGQCEIRHSGFDTHNPLIQADVDRIIDEWFANTDVEAAADPY
                     VAKHLEQIEADQKAAADPDPDPRLADLNSWQAHEQALSTALQEELKAHGYDGGAGDLA
                     GTESHSQLHCEGEDWGGEGGE"
BASE COUNT          723 a         1859 c         1858 g          909 t
ORIGIN      
        1 ctctcgcagc gacacccgcc cgaggcgccc gtcaccccct gtgcgcagcg agatccgggc
       61 gatgcggcga gcgttcacgt ccacctcacc gcgccactcc gtccaggtcc cgccgccggc
      121 gttctccagc ggtcgccagt cgatcgcgcc cggcgcgtcc ttccaggtga tcccgatctc
      181 gacggatgtc agctgctgag cgcgcaccga gaccgccatg gccgcggcag cggtcagatc
      241 ggtcttgaac agatggacga cgccgtcgcc gcgcaaccgg agacagtgcg cgctgccgaa
      301 gctggtggac tcgtccagcg tcacgctcag atcgccctcg gtccagtact gccaggtgag
      361 cgcgcggtcg gcgcagccca tgttgtccca tccgtcggtg cggacctgca ccccgtccag
      421 gtggaacgaa gagccgacgc ccacgttgaa gtcggtgacg atcggcagcg ccccgtaagg
      481 cgagcgctcc gtgatccagt gcgcgatccc gtcccactgc cggtagttca gcgtgtctgt
      541 ccggtacggg ggcgtccgcg gctcgagccg ccctgatgtc accgggttgc cggtgggtcc
      601 ggaccagaac ctctgctcca gatcgcggta ggtgctgcgc ccctccggcg acgaggtctc
      661 gccgcgccag gagccgtcca gccagatctg ggagtccacg aacagcgcca ccgaggtggg
      721 cgccggcttt ccggggcctg cgacgctgcc gaagtcctcc tcgggacgga agccgccctt
      781 ctggtgctcg acgccggcga acccgaccag gcgcgggtcg tatccggcct ccctcgcgga
      841 ggcggcgctc ttggtgaggt cccgatccgc cccgcccgtg gtcgcctgcc accagtagtt
      901 catgaagatc gagtcgtaca tcggcgcgtc cggcgtgccc agccagcgca ggttgttctc
      961 gctgaggcgg ttctcgtagg tcacgaaccc gcggtcgaag tcggccgagt cgtagccctg
     1021 caggtagaag tccgggtcgg tgcgcttgag gtagcgcagg aagtccttga acttcgtgat
     1081 ctgcgcggtg gtgaccgtcg cctcctggtt gatgaacagc ccgtcgaagc cgaagtactt
     1141 ccggatctcg atcagcttgt ccgccacggg gaacgacccg tccgcacgct gtacgaggaa
     1201 cgcgtcgaag tccacagggc ggggccagaa ccagccgccg atggacagag ctccgttctt
     1261 gtgcgccgcc tcggtccaac cgggattcgg gatgtcgatg acgccgtacg gacggccggg
     1321 cacgcgctca ccgtcgacca tctcgatggg cgcgggcccg tcgacctggc cgtgccaggt
     1381 gccccagatg tcgatgtacg cccagtaccg ctgcgtgtac acctgcgcct cgagtccgat
     1441 cgcctggttg tgaccctcgt agatgctccc cgcatcgtcg atcgtgagcg tggagagctg
     1501 cacggccggg tcgaggtccg ggtgcgcctg cgtggcggcg aacggcgcga tgcgcttcgc
     1561 ccgagggatc aggcactgca gatgcctccc ccacggcgtg ctcgcggggt cgaagtcgag
     1621 cagatcgtcc gccgtccagc cgaaggtgta cggcccgggc ggggtgcctc ccgatgcggg
     1681 cacgggtgcc cccttcgcgc tcgcggcggc ggcccttccc gtagccgcgt tcgcggccgt
     1741 gctcgcgttg ccgacgagca gggtcgccag cccgacgcct ccgacggtca ggaacgttct
     1801 ccggttgatc tcggtcatgt gcactctctc cggtctcgca tcttcgcggt tggcgagcgc
     1861 gccgccgagg ggtgggcagc gctgctcgtg ggaacgacta tagcgcttta gtagatcggt
     1921 gccatgcaat gcgtcagcgg atgtcgcgtc ctcggcgcgg acggaaggcg cgaagctcgc
     1981 gcgctccgga cggactgttc cacagcgggc ttcgagcggg cacccatccc cggggcgtac
     2041 cgtcaggcgc gtgttccgac cctctcgtga ccgctggctg atgctgctct gcgcggcatt
     2101 gtccggcgcg gtgctgaccg gctgcgcggc cgcgacggcc gcgaccggat cgcggggcgc
     2161 ggagtctcag cggaaccccg tcatcgtcac gatcggcgac tcgatcatgt ccggtcacgg
     2221 tctcgacgac ggcgaggact ggccgagcat gctctcccag cgcaccggtg tcagcatcga
     2281 caacctcgcc tgcagcgggg cgggtttcct cgcggtgggc acctgcggcc aagacttccg
     2341 gggcctggtg gatgccgccg tgaaggatga tccgaccctc gtcatcgtgc aggggtcgga
     2401 caacgacgtc ggccggtcgg atgccgacat caccgccgcg acgaagcgca cggtcgacgc
     2461 gatccatcgc gccatgcccc gcgcggagct ggtggggctg agcacgctct gggatccgtc
     2521 ggagccgaaa ccagccgaga tcgacgtcag ctccgccgcg gtgcgcgccg ccgtgacagc
     2581 cctcggcggc aggtacatcg acatcggcca gccgctgcgg ggcgatccct cactgctgca
     2641 ggacgacgac gagcatccga ccgtcgcggg acagcagcgg ctcgtgcacg tgatccgcgt
     2701 cgagctggag cacgccggct accggctctg aggccaggcg ctcagttctt gccgaagagc
     2761 ttctggatct cggccatgtc cgcctcggtc ggcgccttct gcccgccgag cccgaaaccg
     2821 gagccggtgg gagtcgagtt cgacatcgcg atgcccgcgt tctcggccgc ccgcttcgcc
     2881 gggttgcccg accgggatcc gcccttgttc ttggcgccct tcttgccgcg cttggcggaa
     2941 gcccccggct tgcccatcgg gcccatgccg gggatctgcg gggtgccgcc gcgggcgacc
     3001 gtcttcatca tcttggcggc ctgctcgaag cgctgcacga gctggttcac gtcggtcacg
     3061 gtcataccgg aaccccgcgc gatgcggagt cgccgcgagc cgttcaggat cttggtgttg
     3121 cggcgctcgg cgggggtcat cgagcggatg atggcctcgg tgcggtcgac ctcgcgctcg
     3181 tcgaagtcct cgagctgctg cttcatctgg cccatgcccg gcagcatgcc gagcatcttc
     3241 ttcatcgagc ccatcttgcg catctgctgc atctgctcga ggaagtcctc gagggtgaag
     3301 gcctcgttgg cgagcttcgc ggccatcttc atggcctctt cctcatcgaa ggcctgctgg
     3361 gcctgctcga tgagggtcag gatgtcgccg aggtcgagga tgcggctcgc catgcggtcg
     3421 gggtggaacg gctcgaggtc ctcgaggttc tcgcccgtgg aggcgaagat gatcgggcgt
     3481 ccggtgacgg acgccaccga cagggcggcg ccaccgcgcg cgtcgccgtc gagcttggac
     3541 agcacgacgc cggtgaagtc gacgccctcc tggaaggcct tggccgtgtt cacggcatcc
     3601 tgaccgatca tcgcgtcgat gacgaacagc acctcgtcgg ggtccgtcgc cttgcggatg
     3661 tcggccgcct gcttcatcag ctcggcgtcg acgccgaggc gtccggcggt gtcgatgatg
     3721 acgacgtcgt gctgctggcg gcgggcgtgc tcgacaccgg ccttggacac ctgcacgggg
     3781 tcgccgacgc cgttgccggg ctcgggggcg aagatcgcag ctcccgcgcg ctcggcgacg
     3841 acctgcagct gcgtgacggc gttcgggcgc tgcaggtcgg cggcgaccag cagcggggtg
     3901 tgcccttcgg cctcgagctg cttggcgagc tttccggcga aggtcgtctt gcccgagccc
     3961 tgcaggccag cgagcatgat cacggtcggc ggggtcttcg cgaactccag acggcgctgc
     4021 tcgccgccga ggatcgcgac gagctcctcg ttgacgatct ggacgacctg ctgggccggg
     4081 ttcagcgcct tgctgacctc atcgctcagg gcgcgctcgc gcaccttcgc ggtgaagtcc
     4141 ttgacgacga cgagcgcgac atccgcgtcg agcagggcgc gacggatctc gcggacggtg
     4201 ccgtcgacgt cggctgggct cagctttccc ttcgtgcgga ggttgcggaa ggtctcggtg
     4261 agccgatcgg agagcgtgcc aaaggttgcc atggtgcctc tgattctacg ggagcggagc
     4321 ctcctgtctg agcgcggccc tcagccgatc cttcgcacgc tggtaacgac cgcgggcggt
     4381 ggatgccggg atgccgagca ggtctgcggc atccgcgacg ctcagacgct cccagtgcac
     4441 cagccggacg agctccgcga ggtcgggctc gagccggtcg atggcgtcgc gcacctcgac
     4501 gccttcatct gcgggcggcg ccgtggcgga ctcccgcacc tgcagccgga tgcgatcggc
     4561 cagcgcccag cgccggcgct gccctcggga gtgattcagc agggtgccac gggcgatgcc
     4621 gaacagccac atgcgtgcgc gttcgctgtc gggcgggagc tccttcaccc ggcgccaggc
     4681 gacgaccatc gtctcgccga gcaggtccgg cgcgtcatcc gcgccgacgc ggcgctcgag
     4741 gtaggcgaga agatcggatg ccgaggactc gagcgcgcgc gtgagcggct cgtcgcggga
     4801 cgtcactcgc cgccctcgcc gccccagtcc tcgccctcgc aatggagctg gctgtggctc
     4861 tccgttccgg cgaggtcccc tgctccgccg tcgtagccgt gcgccttgag ctcttcttgc
     4921 agcgccgtgg agagtgcctg ctcgtgggcc tgccaggagt tgaggtcggc gagccgcggg
     4981 tcagggtcgg ggtctgcggc ggccttctga tccgcctcga tctgctccag gtgcttcgcc
     5041 acatacggat ccgcggcggc ttccacatcg gtgttcgcga accactcgtc gatgatccgg
     5101 tcgacgtccg cctggatcag cgggttgtgg gtgtcgaacc cgctgtgccg gatctcgcac
     5161 tgtccccacg tcggcgacgt gtacgtgtag ctccggtccg gattctccag tccctggatc
     5221 cagagccaat ccgagttggc cgtcgcgatt cccgccccgc cagcaccagc gacaccgacg
     5281 accccgccgc caacgccggg agttttacct ccgagcacgc cgacggtttt gccggtctgc
     5341 acggtgccg
//
LOCUS       DATMSX010000040         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_104803,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000040 DATMSX010000000
VERSION     DATMSX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104803"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VN041_01295"
     CDS             complement(<1..632)
                     /locus_tag="VN041_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27716.1"
                     /translation="MSGRPRIRELDSAALQVTHGLNAVTIGMGVSVVSAFLMVVVLGI
                     VNQELDGLDEVLKLGRDLDGLLRAMFAPILILAGVLLLVGPIGFVWRRAATFAAANRF
                     AAQHPTDAPPRTVREGLRRTPSDALESAGVFGIIASGLLLVVAGVLLIGAVVENPEDI
                     SNLTIFLLSAAVAVLISIVATVRGRRGQPAEKQRALALQQQWKSPAKRAGR"
     gene            complement(667..1530)
                     /gene="truA"
                     /locus_tag="VN041_01300"
     CDS             complement(667..1530)
                     /gene="truA"
                     /locus_tag="VN041_01300"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWU27717.1"
                     /translation="MRIRLDIAYDGTHFRGWAKQPGLRTVQGVLETALARIVGSDVQF
                     VVAGRTDAGVHASDQVAHVDLDEAQWSRVTARHGQAASDPAGSLARRIRGVLGQYPDV
                     TVTRTSLAPEGFDARFSAVWRRYRYRLADTTVGYDPMRRNDTTTIRAALDVDAMDAAA
                     RTLIGLHDFAAYCKPREEATTIRTLLDYRWARDAEGVLVADVKADAFCHSMVRALVGA
                     CAAVGEGKLSVDDVVRLRDELTRTSAFKVLPARGLTLTEVGYPADELLAARAEQTRAR
                     RDRDGDDAGDA"
     gene            1589..>2654
                     /locus_tag="VN041_01305"
     CDS             1589..>2654
                     /locus_tag="VN041_01305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWU27718.1"
                     /translation="MSPSSPHLPRYAGLDGLRAIAVGLVLTYHLFPGLGLRSGLVGVD
                     VFFVVSGFLITSLMLRPTRHPLRHRLLDFWRRRARRLLPALAVMLTVCATVAWLIGGD
                     VLVGIGRQLLGASTFSYNWVALAGGSDYFAATEPELFRNVWSLAVEEQFYLLWPLLLP
                     LLLAVLRVRWVLVAFAVVAAGASAWWMSVLVSTGSVTRAYFGTDSHAFGLLLGIALAF
                     GIRRMPEREWMLRPSVRAGGALLGMLGVGLVVFAAFQPSQPAGTGFPGPLLLASAGTV
                     LAVLAGVMPGSWFGRAIDVQPLRWIGDRSYGIYLWHWPVLVLVSAASGLGVSAQVAGA
                     PVWAGCTALVLSVTTAAVSFR"
BASE COUNT          338 a          957 c          919 g          440 t
ORIGIN      
        1 cggcccgcgc gcttcgccgg cgatttccat tgctgctgga gcgcgagcgc gcgctgcttc
       61 tcggcaggct ggccccggcg cccgcgcacg gtcgcgacga tgctgatcag caccgccact
      121 gccgcggaca ggaggaagat cgtgaggttc gagatgtcct cggggttctc gacgacagcc
      181 ccgatcagga gcaccccggc gaccacgagc agaagtccgg acgcgatgat gccgaagacc
      241 ccggccgact cgagcgcgtc cgagggcgtc ctgcggagcc cctcccgcac cgtgcgcggg
      301 ggcgcgtcgg tcggatgctg agccgcgaac ctgttcgcag cggcgaacgt cgccgcgcgg
      361 cgccacacga agccgatcgg cccgacgagc agcagcacgc ccgcgaggat cagtatcggc
      421 gcgaacatgg cacgcagcag cccgtcgagg tcccggccga gtttcagcac ctcgtccagg
      481 ccgtcgagct cctggttcac gatgccgagc accaccacca tcaggaacgc ggagacgacg
      541 ctgacgccca tcccgatcgt cacggcgttc agcccgtgcg tcacctgcag tgccgccgaa
      601 tcgagctccc tgatccgggg acggccgctc atcctctgac cgaccgatgc gggcatccgt
      661 ctcggctcac gcatcccctg cgtcatcacc gtcccggtcg cggcgagcgc gggtctgctc
      721 ggcccgcgcc gccagcagct cgtcagccgg gtagccgacc tcggtgagcg tcagaccgcg
      781 cgccgggagc accttgaacg cgctcgtgcg ggtcagttcg tcgcgcagcc gcacgacgtc
      841 gtcgacggac agcttgccct cccccacggc cgcgcaggcg ccgaccaggg cgcgcaccat
      901 gctgtggcag aacgcgtcgg ccttcacatc ggcgaccagc acgccctcgg catcccgcgc
      961 ccagcggtag tccagcaggg tgcggatggt cgtcgcctcc tcgcgcggct tgcagtaggc
     1021 cgcgaaatcg tgcaggccga tcagggtgcg ggctgcggca tccatggcat ccacgtccag
     1081 cgcggcgcgg atcgtggtgg tgtcgttgcg acgcatcggg tcgtagccca cggtcgtgtc
     1141 ggcgagccgg taccgatacc ggcgccacac cgccgagaac cgggcgtcga agccctcggg
     1201 cgcgaggctc gtgcgggtga cggtcacatc ggggtactga cccaggaccc cgcgaatgcg
     1261 acgcgcgagc gaaccagccg gatcggaggc agcctgtccg tgccgggccg tgacccgcga
     1321 ccactgcgcc tcgtccagat ccacatgggc cacctggtcg gaggcgtgca caccggcatc
     1381 cgtccttccg gcgacgacga actgcacgtc ggacccgacg atgcgtgcca gcgcggtctc
     1441 gagcacaccc tgcacggtgc gcagaccggg ctgcttcgcc cacccgcgga agtgggtgcc
     1501 gtcgtaggcg atgtccaggc gaatgcgcac ccgtccagcc taaactggag cctcccccct
     1561 cttagaaatg cgcctttctt ctactgtgat gtctccgtct tcgccccacc tcccgcggta
     1621 cgcggggctc gacggactcc gcgcgatcgc cgtcgggctg gtgctgacct accacctctt
     1681 ccccgggctc ggtctgcgca gcggactggt cggtgtggac gtcttcttcg tggtgagcgg
     1741 gttcctgatc acctcgctca tgctgcggcc gacgcggcat ccgctgcggc accgcctgct
     1801 ggacttctgg cgtcgtcggg cacgccggct gctgcccgcg ctggccgtga tgctcaccgt
     1861 gtgcgcgacc gtcgcctggc tgatcggcgg cgacgtgctg gtcggcatcg gccggcagct
     1921 gctgggagcc tcgaccttca gttacaactg ggtggcgctc gcgggcggat cggactattt
     1981 cgccgcgacg gagccggagc tgttccgcaa cgtgtggtcg ctcgccgtcg aggagcagtt
     2041 ctatctgctc tggccgctgc tcctccccct cctgctggct gtgctgcgcg tgcgctgggt
     2101 gctcgtcgcc ttcgcggtgg tcgcggccgg tgcttccgca tggtggatgt cggtgctggt
     2161 ctcgaccggc tccgtcacgc gggcgtactt cggcaccgac tcgcacgcgt tcgggctgct
     2221 gctcggcatc gccctggcat tcggcatccg gcgcatgccc gagcgggaat ggatgctgcg
     2281 ccccagcgtc cgcgccggtg gggcgctgct cgggatgctc ggggtcggtc ttgtggtctt
     2341 cgcagcgttc cagccctcgc agccagcagg gacaggattc ccgggaccgc tgctgctggc
     2401 atccgcggga acggtgctcg cggtgttggc gggcgtcatg cccggttcct ggttcggccg
     2461 cgccatcgat gtgcagccgc tgcgatggat cggcgaccgc tcctacggca tctacctgtg
     2521 gcactggccg gtgctcgtgc tcgtctccgc cgcgagcggc ctgggtgtct ccgcgcaggt
     2581 cgccggagcg ccggtctggg cgggctgcac ggccctggtg ctgtcggtga cgactgcggc
     2641 cgtgtcgttc cgtg
//
LOCUS       DATMSX010000041         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_171140,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000041 DATMSX010000000
VERSION     DATMSX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171140"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VN041_01310"
     CDS             complement(<1..1223)
                     /locus_tag="VN041_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HWU27719.1"
                     /translation="MPCTTKLRVKLNQVVDIIGQALSSEVCSIYLVREGLLELFATRG
                     LKQEAVHVTRMAMGEGLVGLIATNVETLNLDEAAAHPDYSYRPETGEELFHSFAGVPI
                     VRRERAIGVLCVQHVEPRRYEEVEIEALQTVAMVLSELIANAELADDGPADLRVQDTG
                     TIMLQGLQLVMGMARGHAVFHQPRVHVEHTVAEDTEAERQRVYSAFTKMREQIDRMTG
                     AVEFGMEGEHQEVLETYKMFAYDEGWARRINEAIDSGLTAEAAIERVQQRTRMRMRQI
                     DDALLQDRMHDLEDMANRLLRIVSGQLGTAAQLGLRQDAILIARNLGPAELLEYDRRR
                     LKGVILEEGSLTAHVTIVARAMGVPVLGRVREIRQKVNEGDLILMDVVSNSLLIRPTP
                     DMDEAFENKLHVTQKR"
     gene            1195..1509
                     /locus_tag="VN041_01315"
                     /pseudo
     CDS             1195..1509
                     /locus_tag="VN041_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
     gene            1553..1908
                     /gene="rnpB"
                     /locus_tag="VN041_01320"
     ncRNA           1553..1908
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN041_01320"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2046..2345
                     /locus_tag="VN041_01325"
     CDS             2046..2345
                     /locus_tag="VN041_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27720.1"
                     /translation="MNTLAIQFAVAAVMVPVGIAGAVIAALSAVITCVVLIRGSDEIG
                     IPVAGWIVGTILSLAAAFVELTPTLVSAAAGAVVVVVALVVLGGRRAHSRRLHTR"
     gene            complement(2418..3164)
                     /locus_tag="VN041_01330"
     CDS             complement(2418..3164)
                     /locus_tag="VN041_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU27721.1"
                     /translation="MAQQAIGVDIGGTGIKAGIVNLEHGTLDSDRVRVDTPKGARPAD
                     VLDTVREVLDTLGVTGSQLPLGVAFPAIVKRGRTLSAANIAQDWVGFEAEKFFEDGLG
                     RHIAFVNDADAAGVAEARHGAARDAMGLTILTTLGTGIGSAFLYDGVLVPNTELGHIK
                     REGESIEKWTAYSAMERESLSWEAWGERLQEFYSQIEFLFSPDLFVVGGGVSKHPEKF
                     LPLLELNTPIVAAVHRNAAGIIGAASLAAD"
     gene            3232..3456
                     /locus_tag="VN041_01335"
     CDS             3232..3456
                     /locus_tag="VN041_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWU27722.1"
                     /translation="MADGDDKYWYNLSTGEVEFGMLSASVDRVGPFDTADEAAHAPEK
                     LRERSRAWAEDDAAEDSWGSASRADSGDDE"
     gene            3459..4796
                     /locus_tag="VN041_01340"
     CDS             3459..4796
                     /locus_tag="VN041_01340"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWU27723.1"
                     /translation="MDKQRDFVLRTIEERGVKFVRLWFTDVIGTLKSVAIAPAEVEGA
                     FAEGIGFDGSAIEGLTRTFESDLLAQPDPSTFQILPWRGEVDPTARMFCDLTTPDGRP
                     AVSDPRNVLRRALAKAADAGFTFYTHPEIEFYLLKSSQVGPDGPVPVDSAGYFDNVPG
                     GTAHDFRRSAVRMLEDLGISVEYSHHEGGPGQNEIDLRYADALTMADNIMTFRTVVKE
                     VAIEQGVYATFMPKPLNNHPGSGMHTHLSLFEGDVNAFYEAGAEYQLSLTGRRFIAGL
                     LRHANEIAAVTNQFVNSYKRLWGGDEAPSFVTWGHANRSALIRVPMYKPNKGQSTRVE
                     YRALDSAANPYLAYALMLAAGLKGIEEEYELPPEAEDNVWSLSDSERRALGYAPLPAS
                     LDHALEYMQDSELVAETLGEQVFNYVLLNKRKDWEAYRGQVTPFELQNNLGLL"
     gene            4806..>5033
                     /locus_tag="VN041_01345"
     CDS             4806..>5033
                     /locus_tag="VN041_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27724.1"
                     /translation="MARPDASVSLSALARLGFVELTAASAALTELAELTGLERADLVE
                     GLGSADPDAAAAGILRIARRDPSRVRTALQEI"
BASE COUNT          817 a         1729 c         1672 g          815 t
ORIGIN      
        1 cgcttctgcg tgacgtgcaa cttgttctcg aacgcctcgt ccatgtcggg cgtcgggcgg
       61 atcagcaggc tgttcgacac aacgtccatc aggatgaggt cgccctcatt caccttctgc
      121 cggatttcgc ggacgcgccc caataccggt acgcccatgg cgcgtgccac gatggtgacg
      181 tgcgcggtca gcgatccttc ctccagaatt acacccttca agcgccgccg gtcatattcc
      241 agcagttccg ccggccccag gttgcgcgcg atcaggatgg catcctgcct cagccccagc
      301 tgcgccgccg tgcccagctg tcccgagacg atccgcaaca gccggttggc catgtcctcc
      361 agatcatgca tccggtcctg caacaacgca tcatcgatct gccgcatgcg catccgggtg
      421 cgctgctgca cgcgctcgat cgccgcctcg gcggtcaggc cgctgtcgat cgcttcgttg
      481 atgcgccgcg cccaaccttc gtcataagcg aacatcttgt aggtttcgag cacctcctga
      541 tgctcgccct ccatgccgaa ttcgaccgcg ccggtcatgc ggtcgatctg ctcgcgcatc
      601 ttggtgaagg cggaatagac gcgctgccgc tccgcctcgg tatcctccgc gaccgtatgt
      661 tcgacatgga cgcgcggctg gtggaacaca gcatgccccc gcgccatgcc catcaccagc
      721 tgcaatccct gcagcatgat cgtgcccgtg tcctgcaccc gcaggtccgc cgggccatca
      781 tccgccagct cggcattggc gatcagttcc gacagcacca tcgccacggt ctgcaacgcc
      841 tcgatctcga cctcttcata gcggcgcggc tcgacatgct ggacgcacaa cacgccaatc
      901 gcccgttccc gtcgcacgat cggcacgccc gcaaagctgt ggaacagctc ttcgcccgtt
      961 tccggccgat agctatagtc cgggtgcgcc gccgcctcat ccaggttcag cgtctcgaca
     1021 ttggtcgcga tcaggccgac cagaccttcg cccatcgcca tgcgcgtgac gtggacggcc
     1081 tcctgcttca gcccgcgcgt cgcgaacagt tccagcagcc cttcgcgcac cagatagata
     1141 gagcaaacct cgctcgacag cgcctggccg ataatgtcga cgacctggtt gagcttcacc
     1201 cgcaacttcg tcgtgcaggg cacggccgcg gagtgggcgc tgctgtggct cgccgagata
     1261 cggcatcgcc tgatggcgct gccagtggca tccgctctcg ctccggcatc cggcccggtc
     1321 ttctccaggc gggcgcacct ggcgttcttc ctgcacgacg aggtgatcgt gcactgcccg
     1381 gcggaacagg cggatgccgt ggcatcggcc gtgcgcggcg cagccgaggc ggcgacagcg
     1441 cgactgttcc ccgggttcgc gatcgatgtg ccgctggatc tgcacatcag cgccgacgcg
     1501 gggaagtgac gcgcgtcccg gtagaagcgt cccggtagaa tcggagggcg agcgggtcgg
     1561 ccggacggtc gcgtggcgcg agagcgcacc gaggaacgtc cgggctccac agggcagggc
     1621 ggtgggtaac acccacccgg agtgatccgc gagaaagtgc cacagagaga agaccgcccc
     1681 ctcgggggta agggtgaaag ggtggtgtaa gagaccaccg ggggacatgg tgacatgtcc
     1741 cgccaggtaa acctcgcccg gagcaagacc agacagagga cgtcgacgcg gcccgccgag
     1801 tcctcgggta ggttgctgga gcggcacggc aacgtgtcgc cgagagagat ggccgtcaag
     1861 ggagcgcgag ctcctgaaca gaacccggcg tacaggccgg cccgctcgca ttcagacccg
     1921 tccgacaggg gttacccggt cggacgggtc tcttcgtctt cgggaggatc gtgattcccg
     1981 cgtaatgaaa gggccggtac gacgtctctt cctgtgtcgg atacgcacac cacgcaggga
     2041 gcacgatgaa cacgctggcc atccagttcg cggtcgcagc cgtgatggtg cccgtcggga
     2101 tcgcaggggc ggtgatcgcc gcgctcagcg cggtcatcac ctgcgtcgtg ctgatcaggg
     2161 ggagtgacga gatcggcatc cccgtcgccg gatggatcgt ggggacgatc ctgagcctgg
     2221 ctgcggcctt cgtcgagctg acgcccaccc tcgtgtcggc ggccgcgggt gccgtggtgg
     2281 tcgtggtcgc gctcgtcgtg ctcggcggac ggcgggctca ctcccgccga ctgcacacgc
     2341 gctgacgcgc gcgcgagctc cacaggcata cgaaaggccc cgatcagatg atcggggcct
     2401 ttcggcgttc tcgggtctca gtcggcggcg agcgacgcgg cgccgatgat gccggccgcg
     2461 ttgcgatgca ccgcagcgac gatcggggtg ttcagctcga gcagcggcag gaacttctcc
     2521 ggatgcttgg agacgccgcc gccgacgacg aacaggtcgg ggctgaacag gaactcgatc
     2581 tggctgtaga actcctgcag ccgctcgccc caggcctccc aggacaggct ctcgcgctcc
     2641 atggcggagt aggcggtcca cttctcgatg gactcgccct cgcgcttgat gtggccgagc
     2701 tccgtgttcg gcacgagcac gccgtcgtag aggaacgcgg agccgatccc ggtgcccagc
     2761 gtggtcagga tcgtgagtcc catggcatcc cgtgccgcgc cgtggcgcgc ctcggcgacg
     2821 cccgcagcat ccgcgtcgtt gacgaacgcg atgtgacggc ccaggccgtc ctcgaagaac
     2881 ttctcggcct cgaagccgac ccagtcctgt gcgatgttcg ccgccgacag cgtccgtccg
     2941 cgcttgacga tcgcggggaa ggccacgccc agcggcagct gcgaacccgt cacacccagc
     3001 gtgtccagca cctcgcggac ggtgtccagc acatccgcgg gacgtgcgcc cttgggcgtg
     3061 tcgacacgca cgcgatccga gtcgagcgtg ccgtgctcga ggttgacgat tcctgccttg
     3121 atgccggtgc cgccgatgtc gacgccgatt gcctgctgtg ccatgccgaa cagcctatcc
     3181 agaacggtcg ggacgattag gatcgtgaca gatcccgacg aaggagctga catggccgac
     3241 ggcgacgaca agtactggta caacctgagc acgggcgagg tggagttcgg catgctctcc
     3301 gcctcggtcg accgggtggg tccgttcgac acggccgacg aggccgcgca cgcccccgag
     3361 aagctgcggg agcgatcccg tgcctgggcg gaggacgacg ccgccgagga ctcctggggc
     3421 agcgcgtccc gcgccgactc cggcgacgac gagtgagcat ggacaagcag cgcgatttcg
     3481 tcctccgcac gatcgaggaa cgcggcgtca agttcgtgcg cctgtggttc accgatgtga
     3541 tcggcactct caagtcggtg gcgatcgcac cggccgaggt ggagggcgcg ttcgcggagg
     3601 gcatcggctt cgacggctct gcgatcgagg gcctgacccg caccttcgag tcggacctgc
     3661 tcgcgcagcc cgacccgtcc accttccaga tcctcccgtg gcgcggcgag gtcgacccga
     3721 ccgcgcggat gttctgcgat ctgacgacgc cggacgggcg tcccgcggtc tccgacccgc
     3781 gcaacgtgct gcgccgtgcg ctggcgaagg cggcggatgc cgggttcacg ttctacacgc
     3841 atccggagat cgagttctac ctgctgaagt cctcgcaggt ggggcccgac ggaccggtcc
     3901 cggtcgactc ggccggctac ttcgacaacg tgcccggggg caccgcgcac gacttccgcc
     3961 gcagcgccgt gcggatgctg gaggacctcg gcatctcggt cgagtacagc caccacgagg
     4021 gcggtcccgg tcagaacgag atcgatctgc gctacgcgga cgccctcacc atggccgaca
     4081 acatcatgac cttccgcacg gtcgtgaagg aggtcgcgat cgagcagggc gtgtacgcga
     4141 cgttcatgcc gaagccgctc aacaaccacc cgggcagcgg catgcacacg cacctttcgc
     4201 tgttcgaggg cgacgtcaac gcgttctacg aggcgggcgc ggagtatcag ctgtcgctca
     4261 ccggccggcg gttcatcgcg ggcctgctgc ggcacgccaa cgagatcgcc gcggtgacca
     4321 accagttcgt caactcgtac aagcgcctgt ggggcggcga cgaggcgccc agcttcgtca
     4381 cctgggggca cgcgaaccgc tcggccctca tccgcgtgcc gatgtacaag ccgaacaagg
     4441 gccagtcgac gcgcgtggag taccgcgctc tcgattccgc cgcgaacccc tacctcgcct
     4501 acgcgctgat gctggccgcc ggtctgaagg gcatcgagga ggagtacgag ctcccgcccg
     4561 aggcggagga caacgtctgg tcgctgagcg actcggagcg gcgcgccctc gggtacgcac
     4621 cgctgccggc gagcctggac cacgcgctgg agtacatgca ggactccgag ctggtcgccg
     4681 agacgctggg ggagcaggtg ttcaactacg tgctgctgaa caagcgcaag gactgggagg
     4741 cgtaccgagg gcaggtcact cccttcgagc tgcagaacaa cctcgggctg ctctgagtcc
     4801 cagtcatggc acgacctgat gcgtccgtct cgctgtcggc gctggcccgg ctcggcttcg
     4861 tcgaactgac ggcggcgtcg gccgcgctca ccgaactcgc cgagctgacc gggctggagc
     4921 gcgccgacct cgtggagggc ctgggttccg cggatccgga tgccgctgcc gccggcatcc
     4981 tgcgcatcgc ccgtcgcgat ccctcccggg tcaggacggc gctgcaggag atc
//
LOCUS       DATMSX010000042         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123817,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000042 DATMSX010000000
VERSION     DATMSX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123817"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..323
                     /locus_tag="VN041_01350"
     CDS             150..323
                     /locus_tag="VN041_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HWU27725.1"
                     /translation="MSEDKAVSPEEMKRKFKEALDRKNAKNQGGEAHLDGDSAVHATP
                     APQMKREFRRKSG"
     gene            complement(415..1812)
                     /locus_tag="VN041_01355"
     CDS             complement(415..1812)
                     /locus_tag="VN041_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HWU27726.1"
                     /translation="MAMTSGFGFPRLPRRRGRKSWDRHTWLYVSVLIAVVLGALVGLI
                     WPEVGQSFEALGKGFVALIKMMIAPIIFCTIVVGVGSIAKAATVGRIGGLALIYFMVM
                     STFALAIGLVVGNIIHPGSGLDMAGSSYQAASTEASTAQDFVLGIIPTTFFSAFTGES
                     VLQVLFIALLAGFALQGLGDRGAPIMEAVKNLQLLVFRILGMILWLAPVGAFGAIAAV
                     VGKTGLAAIWSLGILMVAFYITCIVFIVVVLGTVLWLGSRVSIFSLMRYLGREYLLIV
                     GTSSSESALPRLIAKMEHVGVSKPVVGITVPTGYSFNLDGTAIYLTMASLFIATGMGQ
                     PMSITEQIGLLVFMILASKGAAGVTGAGLATLAGGLQAYRPDLVDGVGVIVGIDRFMS
                     EGRALTNFTGNAVATLLIGTWTKQIDRDRVRLVLSGELPFDESSLDGVHSHDVAKAHT
                     EGAQAESAQVGVAQR"
     gene            1958..3229
                     /locus_tag="VN041_01360"
     CDS             1958..3229
                     /locus_tag="VN041_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU27727.1"
                     /translation="MKTVAPSRSAASRVFVVLIAASLILAALVAAFLVFETQTAAHDE
                     AERVTAATARTLAVSPLVAESLEDDRQAASAALEPYALAVTSRAGLDFITVMTPDGTR
                     VTHPDPDEIGKHYRGTIPAEGRELTEVFTGTLGPSVRTIVPVTDTAGRLVGWVAAGVT
                     TESISETLVRRLPLTIAITAALVLLGAVGSMAARRFTRRIAGDLPAGEVRDAVSSYES
                     FRTLGEALRAQTHEHGNRMHTAVALLELGRTDEAIEILTETSRQSQTLVDQVTAQRHG
                     DPAVGALLLGKVSQARERGIDLRLHIAQDAPSSGLSPIDSVSVIGNLIDNAFDAAAAS
                     DDRWVEVGMRPAADGGLVIAVSDSGTGVPVELREQVFSPGFSTKPAGAEGRGVGLALV
                     RSVVAEAGGAVRILDDPSTFLVELPIPRSRR"
     gene            3226..>3762
                     /locus_tag="VN041_01365"
     CDS             3226..>3762
                     /locus_tag="VN041_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU27728.1"
                     /translation="MIRTLLVDDDALTMELHRSYLARMSGFEIAGECTSARAAMTAVL
                     DRPGAQAFDLALLDITMPDGSGVDVLRTIRTRAAAIDVIAITGVRDAETVRQMAALGV
                     FQYLVKPFPFAVFSERLSAYREYKQRALATEGEATQTEIDALLGRVRPAVPLPKGLAA
                     ETLERVSAVLRGSGPLSAT"
BASE COUNT          593 a         1326 c         1291 g          552 t
ORIGIN      
        1 gttgtcgcgg cgctcctccc gggcggcccg ctggtggcgg tggtcgtgtc cgtggtcctc
       61 gcggtcgccc tgggggctgc catcgacctg atcacggcgc ggcggtcgcg ctcgcgtccc
      121 gcggatgccg accggctagg ctgacggaca tgagcgaaga caaggcagtc tcccccgagg
      181 agatgaagcg caagttcaag gaagcgctcg accggaagaa cgcgaagaac cagggcggcg
      241 aggcgcacct cgacggcgat tccgccgtgc acgcgacccc cgcgccgcag atgaagcgcg
      301 agttccgccg gaagagcggc tgagcccgta cccacgtacg acgcaggccc gcgatgctgc
      361 tgcatcgcgg gcctgcgtcg ttccgcgcgg tgatctccgg gcatcccgca ccgctcagcg
      421 ctgcgcgacg ccgacctgtg cgctctcggc ctgtgcgccc tcggtgtgcg ccttcgcgac
      481 gtcgtggctg tggacgccgt ccagcgagga ttcgtcgaac ggcagctcgc cgctgagcac
      541 gaggcgtacg cgatcccggt cgatctgctt cgtccaggtg ccgatcagca gcgtggcgac
      601 ggcgttgccg gtgaagttcg tcagcgcgcg cccctcggac atgaagcggt cgatgcccac
      661 gatcacgccg acgccgtcga ccaggtcggg acgatacgcc tgcagaccac cggccagcgt
      721 cgcgagtccc gctcccgtga cgcccgccgc gcccttgctg gcgaggatca tgaacaccag
      781 caggccgatc tgctccgtga tcgacatcgg ctgtcccatt ccggtcgcga tgaacagcga
      841 ggccatcgtg aggtagatcg cggtgccgtc caggttgaac gagtagccgg tgggaacagt
      901 gatgccgacg acgggcttgg agacgccgac gtgctccatc ttcgcgatca ggcgcgggag
      961 ggcggactcc gacgacgagg tgccgacgat gagcaggtat tcacggccga ggtaccgcat
     1021 caggctgaag atgctcaccc gggagcccag ccacagcacc gtgcccagca cgaccacgat
     1081 gaagacgatg caggtgatgt agaacgcgac catgaggatg ccgaggctcc agatcgccgc
     1141 cagaccggtc ttcccgacga ccgccgcgat cgcgccgaac gcaccgaccg gagccagcca
     1201 gaggatcatg ccgaggatgc ggaagaccag cagctgcagg ttcttcaccg cctccatgat
     1261 cggcgcaccg cggtcgccga ggccctgcag cgcgaatccg gcgagcagtg cgatgaacag
     1321 cacctgcagc acgctctccc cggtgaacgc ggagaagaac gtcgtgggga tgatcccgag
     1381 cacgaagtcc tgcgcggtgg atgcctcggt ggacgcggcc tggtaactgg agccggccat
     1441 gtcgagcccc gaacccgggt ggatgatgtt cccgaccacg agtccgatgg cgagcgcgaa
     1501 ggtcgacatc accatgaagt agatcagcgc gagcccgccg atccgaccga ccgtcgccgc
     1561 cttcgcgatg gagccgaccc ccaccacgat cgtgcagaag atgatcggcg cgatcatcat
     1621 cttgatgagg gcgacgaagc ccttgcccag cgcctcgaag ctctgcccca cctcgggcca
     1681 gatcagcccg acgagtgcgc cgagcaccac cgcgatcagc accgacacat agagccaggt
     1741 gtgcctgtcc cacgacttcc tgccgcgccg ccgcgggagc ctcgggaacc cgaatccgct
     1801 tgtcatcgcc atgtttcctc ctcgatcatg aacgtcgtcg tgtcatgccg gacggcgtcc
     1861 tcgccgttcg ttcctccacc gtcacggact tctcatcggg gtgccatttg tggtcgtatt
     1921 ggtcgcgagc agcgcagcgg acgacgggag ggcgtggatg aagaccgtcg ccccgtcgcg
     1981 cagcgccgca tcccgcgtct tcgtggtcct gatcgcggca tccctgatcc tcgcggcgct
     2041 ggtggccgcg ttcctcgtct tcgagacgca gactgccgcg catgacgagg cggagcgcgt
     2101 caccgcggcg accgccagga cactcgccgt ctctcccctc gtcgccgagt cgctggagga
     2161 cgaccgtcaa gcggcatccg cagccctcga gccctacgca ctggcggtca cctcccgagc
     2221 gggcctggac ttcatcaccg tcatgacgcc ggacggcacg cgcgtcacgc atcccgaccc
     2281 cgacgagatc ggcaagcact accgcggcac gatccccgcc gagggccggg agctgacaga
     2341 ggtcttcacc ggcacgctgg ggccctcggt gcgcacgatc gtgccggtga cggataccgc
     2401 gggccggctc gtcgggtggg tggccgcggg cgtcacgacc gagtccatct cggagacgct
     2461 ggtgcgccgc ctccccctca cgatcgcgat caccgccgcg ctcgtcctgc tgggcgccgt
     2521 cgggtcgatg gcggcgcgtc gcttcacccg ccgcatcgcg ggcgacctgc ccgccggaga
     2581 ggtccgtgat gcggtgtcct cgtacgaatc gttccgcaca ctgggagaag cgctgcgggc
     2641 gcagacccac gagcacggga accgcatgca caccgcagtc gcgctgctcg agctgggccg
     2701 caccgacgag gcgatcgaga tcctcaccga gacgtcccgg cagagccaga cgctcgtgga
     2761 ccaggtgacg gcgcagcgcc acggcgaccc agcggtgggc gcgctgctgc tgggcaaggt
     2821 gtcccaggcg agggagcgcg gcatcgacct gcgcctgcac atcgcgcagg atgccccgtc
     2881 ctccgggctc tcacccatcg acagcgtgtc cgtgatcggc aatctcatcg acaacgcctt
     2941 cgacgcggcg gccgcctccg acgaccgctg ggtcgaggtc gggatgcgcc ccgctgcgga
     3001 cggcggcctc gtgatcgcgg tgtcggacag cggcaccggt gtgccggtcg agttgcgcga
     3061 gcaggtcttc tcgccgggct tctccaccaa gccggccggg gcggagggcc gcggcgtcgg
     3121 gctggcgctc gtgcgatccg tcgtcgccga ggcgggcgga gcggtccgca tcctggacga
     3181 tccgagcacg ttcctggtcg agctccccat cccgcggagc cgccgatgat ccggacgctg
     3241 ctggtcgacg atgatgcgct caccatggag ctgcaccgca gttacctcgc gcgcatgagc
     3301 gggttcgaga tcgcaggcga gtgcacgagc gcgcgggcgg cgatgacggc ggtgctggac
     3361 cgcccgggag ctcaggcgtt cgacctcgca ttgctggaca tcaccatgcc cgacggctcg
     3421 ggcgtcgatg tgctgcgcac catccgcacg cgggcggccg cgatcgacgt catcgcgatc
     3481 accggtgtgc gggatgccga gacggtgcgg cagatggcgg cgctgggcgt gttccagtac
     3541 ctcgtgaagc cgtttccgtt cgcggtcttc agcgaacgcc tcagcgccta ccgggagtac
     3601 aagcagcgcg ccctggccac cgagggcgag gccacgcaga ccgagatcga tgcactgctg
     3661 ggccgcgtgc gccccgccgt cccccttccc aagggtctgg cggcggagac gctggagcgg
     3721 gtgtccgccg tgctgcgcgg atccggtccg ctgtccgcga cc
//
LOCUS       DATMSX010000043         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_152193,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000043 DATMSX010000000
VERSION     DATMSX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152193"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VN041_01370"
     CDS             complement(<1..1116)
                     /locus_tag="VN041_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWU27729.1"
                     /translation="MGPTSDTPLRPQDSGLLPRLLAELGLRPDTLTLPARAVQAVPLP
                     SHLAVGELAWTSIGAAAAAIGTASADDDWHPLLPDPDLVADAYRSDRYLRVDGEAIEG
                     FAPLSGFWRTADGWVRTHGNYAHHAAALRTGLRLRNGATPDEVASVLRTMTSVQAMTA
                     VQAHGGLCVPVRQEAPREDAALRATPLIQVSRLGDSRPRALPRPTAASPLSGIHVLDL
                     TRVIAGPVATRTLAFAGAEVLRLDPPMREEISWQHFDTGHGKRTALLDIAADRERFDA
                     LLADADVIVLGYRPDGLRRLGLDPQSLVSRHPQLVVAQLSAWPGDDSARGFDSIVQAA
                     CGISWADSVDGQLPGALPAQALDHSAGYLLAAGITAAL"
     gene            1604..>1712
                     /locus_tag="VN041_01375"
     rRNA            1604..>1712
                     /locus_tag="VN041_01375"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          291 a          515 c          613 g          293 t
ORIGIN      
        1 cagcgctgcg gtgatccccg cggcgagcag gtatccggcc gaatggtcca gcgcctgcgc
       61 gggcagcgcg ccggggagct gtccgtcgac cgagtccgcc catgagatgc cgcaggccgc
      121 ctgcacgatg ctgtcgaacc ctcgggcgga atcgtcgccg ggccaggcgc tgagctgcgc
      181 cacgacgagc tgcggatgcc gggagaccag cgactgagga tccaggccga gccggcgcag
      241 tccgtccgga cgataaccga gcacgatcac gtcggcgtcg gcgagcagcg catcgaagcg
      301 ctcgcgatct gcggcgatgt ccaggagagc ggtccgcttg ccgtgaccgg tgtcgaagtg
      361 ctgccagctg atctcctccc gcatcggagg gtcgagtcgc agcacctcgg caccggcgaa
      421 cgccagagtg cgcgtcgcga cgggaccggc gatcacccgg gtcagatcga gcacgtggat
      481 gcccgacaga ggcgaggccg ccgtggggcg gggaagtgca cgcggccggg agtcgccgag
      541 tcgggaaacc tggatcagcg gagtggctcg cagcgcggca tcctcacgcg gggcctcctg
      601 tcgcacagga acgcagagac cgccgtgcgc ctggacggcg gtcatcgcct gcacggatgt
      661 catggtgcgc agcaccgaag cgacctcgtc gggggtggcg ccgttccgaa gacggaggcc
      721 cgtgcggagg gcggcggcat ggtgcgcgta gttgccgtgc gtgcgcaccc atccgtcggc
      781 ggtacgccag aacccggaga gaggagcgaa cccctctatc gcttcgccgt cgacgcgcag
      841 gtaccggtcg ctgcgatacg cgtcggccac gaggtcggga tcgggcagga gcggatgcca
      901 gtcatcatcc gcagaggccg tcccgatcgc agcggcggcg gctccgatgc tggtccaggc
      961 gagctcgcca acggcgagat gggagggcag aggtacggcc tgcaccgcgc gggcggggag
     1021 cgtgagagtg tcggggcgca gccccagctc cgccagcagg cgggggagga gcccggaatc
     1081 ctgcggccgc agcggggtgt cactggtggg tcccatgacg cgagactacg tcggccggag
     1141 ccgcgacacg cccgggagac ggggctgatt tgccggatgc ccgggatcca cgtaaattat
     1201 tacttgttcg ccccaagcgg ttgagcggaa ggcctgaagg cctggctccc ctccaagtag
     1261 cgaacacaaa tcctcaactt ccaccggcgg cttcgctggt gtaagatgga ggttccggct
     1321 ccggaacgac tggtcaacag tcctggatct gccgggtaag attgtgaagt tgccctgcgc
     1381 ttctcgccct gttggggtgg gtgtgtgggt gcgtccgatc cttgagaact caacagcgtg
     1441 cacttgtcaa atgccaaatt ttcctcgtcc agccctgtgg ggttggttga gaattccttt
     1501 ggatcaattt atatgaatgt cagcaatgat gttcgtctct tttggtcatg attgaactcg
     1561 ctgccttctc accgttttcc cggtggttgg tatgcatctg ttttttacgg agagtttgat
     1621 cctggctcag gatgaacgct ggcggcgtgc ttaacacatg caagtcgaac gatgaagccc
     1681 agcttgctgg gtggattagt ggcgaacggg tg
//
LOCUS       DATMSX010000044         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_38595,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000044 DATMSX010000000
VERSION     DATMSX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38595"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VN041_01380"
     CDS             complement(<1..559)
                     /locus_tag="VN041_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HWU27730.1"
                     /translation="MTERGFAATSRVDDAGMALALAPALTPTGPQRAPEFFRGFATRP
                     QVLARALVTLADITAMRYFQYTPTTQRDPVLSAHGDRLRAECFSACNSVYARLDVFGD
                     GLDGGEIGFGTTNVDITADTRRMLSGVGHTELLHLQVGDAGLTAATPEAQSQERPVRM
                     PTRWVPALGNVAELHRGTSPGFTLDA"
     gene            615..1259
                     /locus_tag="VN041_01385"
     CDS             615..1259
                     /locus_tag="VN041_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU27731.1"
                     /translation="MTEIDDGLTRWSAGERARRPLLVLLHGYGADENDLFGLVPYLPD
                     GLAVASVAAPLTPPWPMPGRSWYPIDSLNTRDAAGITLAAEGLLRWLDAVAADAAHVA
                     LLGFSQGAAVALQALRLAPERIDAVVVLSGYAAPGDLPNDAALQERKPPVFWGRGSRD
                     DVIPPNLIDHTAQWLPSHAELSGRVYPGLTHSISEQELSDVDVFLSQWKDSLDT"
     gene            complement(1263..1544)
                     /locus_tag="VN041_01390"
     CDS             complement(1263..1544)
                     /locus_tag="VN041_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27732.1"
                     /translation="MDDRQPSPHEMRARMGFAGSIVIGLGVAAALGTATNSMPIGLGV
                     GLAVTVVLMVVFTRAGSRKRRPSAAGSTGPDAADTDREHRPDDDADTPA"
     gene            1639..2448
                     /locus_tag="VN041_01395"
     CDS             1639..2448
                     /locus_tag="VN041_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27733.1"
                     /translation="MANESRLSIGTTKARQREIAFCASAVPLLWLTMLRSSDLQRSGQ
                     VTFAVELDTALTRLADPSGARKMFAEYGDLAPFAALLAETLAEEAGPLVTVSADEIAD
                     EHPDPRAWARSLAVALDSLDDPGLAAAPFDPPSPAVPAMTELQKMQRVRDASTLGLQD
                     ARALLHAHDGDLHAALAHIEAGKSAEQRARDQAGDFVASLISPHPVTDAPPWRTIMSA
                     CGLEHGRRFPRPDLLLAPLSTADSEDQRVHTRLLGEGTSADVVAWEPHASP"
     gene            2697..>2887
                     /locus_tag="VN041_01400"
     CDS             2697..>2887
                     /locus_tag="VN041_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27734.1"
                     /translation="MSTPTTFTSAAVTLDRLTFAWADGPVALDAVSGSFGSGRTGLVG
                     RNGAGKSTLLRLIAGDLAPT"
BASE COUNT          429 a         1053 c          947 g          458 t
ORIGIN      
        1 cggcgtccag cgtgaagccc ggcgacgtgc cgcgatgcag ctccgcgacg ttacccagcg
       61 ccggcaccca ccgcgtcggc atcctgaccg gccgctcctg cgactgcgcc tcgggcgtcg
      121 ctgcggtcag acccgcatcg ccgacctgca ggtgcagcag ctcggtgtgc ccgacgcccg
      181 acagcatccg cctcgtgtcg gcggtgatgt caacgttcgt ggtgccgaac ccgatctcgc
      241 ccccgtcgag gccgtcgccg aacacgtcga gccgcgcgta gacgctgttg caggcggaga
      301 agcattcggc tcgcagccga tcgccgtgcg cgctcagcac cgggtcgcgc tgcgtggtcg
      361 gcgtgtactg gaagtagcgc atggccgtga tgtccgccag cgtgacgagg gcgcgggcga
      421 gcacctgggg gcgtgtcgcg aagcctctga agaactccgg agcgcgctga ggaccggtcg
      481 gcgtcagagc gggcgcgaga gcgagcgcca tgcctgcatc gtcgacacgg gaggtcgctg
      541 cgaagccccg ttcagtcacg ctcatcaccc tagggggcct gaccgacgtc cgtcgtccct
      601 ggcaggatga ggtcatgacc gagatcgacg acggcctgac gcgctggtct gctggtgagc
      661 gcgccaggag acccctgctc gtgctgctgc acggctacgg cgccgacgag aacgacctgt
      721 tcgggctggt tccctatctc cccgacgggc tcgcggtggc atccgtcgcc gccccgctga
      781 caccgccctg gccgatgccg ggccgctcgt ggtacccgat cgactctctg aacacgaggg
      841 atgcggctgg catcacgctg gccgccgaag gactgctgcg ctggctggac gccgtcgccg
      901 ccgacgcggc gcatgtcgca ctgctgggct tctcgcaggg tgctgccgtc gcgcttcagg
      961 cgctgcggct cgcgccggag cggatcgacg cggtggtcgt gctcagcggg tacgccgctc
     1021 ccggcgatct gccgaacgac gccgcactgc aggagcggaa gccgccggtg ttctggggcc
     1081 gcggaagccg cgacgacgtg atcccgccga acctcatcga ccacaccgcg cagtggctgc
     1141 cctcgcacgc cgagctctcc gggcgggtgt acccgggtct cacccactcc atctcggagc
     1201 aggagctgtc cgatgtcgac gtgttcctgt cgcagtggaa ggactcgctc gacacctgac
     1261 gctcaggccg gtgtgtcggc gtcgtcgtcg ggtctgtgct cgcgatcggt gtcggcggca
     1321 tccggtccgg tgctcccagc ggcacttgga cgccgcttcc gcgaccctgc acgggtgaac
     1381 accaccatga gcacgacggt cacggcgagg ccgaccccga gtccgatcgg catgctgttc
     1441 gtcgcggtgc cgagcgcagc ggcgacgccg agaccgatga cgatgctccc cgcgaagccc
     1501 atacgggcgc gcatctcgtg cgggctcggc tgcctgtcat ccatggctcc accgtacgca
     1561 ccggccggac gcctggcatc caccacacgg cggatatctc tcgcggggga agtgcgccgt
     1621 cgtgatagaa atcaaggcat ggcgaatgag agccgcctca gcatcggtac gacgaaggca
     1681 cgccaacgtg aaatcgcctt ctgcgcgtcc gcagtgcccc tcctctggct gacgatgctg
     1741 cggtcgagtg accttcagcg gtcgggccag gtcaccttcg ccgtcgaact cgacaccgca
     1801 ctcaccaggc tcgcggatcc gtccggcgcc cgcaagatgt tcgccgagta cggcgacctt
     1861 gccccctttg cggcactgct cgcggagaca ctggcggagg aggcgggccc gcttgtcacc
     1921 gtctcggctg acgagatcgc cgatgagcat ccggatcctc gcgcctgggc gaggtcgctg
     1981 gccgtcgcgc tcgacagcct cgatgatcca ggactcgcag cggctccctt cgacccgcca
     2041 tcccctgccg tgcccgcaat gaccgaactg cagaagatgc agcgggtacg ggacgcctcc
     2101 acgctcggac tacaggacgc tcgggcgctt ctccacgctc atgatgggga ccttcacgcc
     2161 gcgctcgcgc acatcgaggc aggaaagagc gccgagcaaa gggcccgcga tcaggccgga
     2221 gatttcgtgg caagcctgat atcaccgcat ccggtgaccg atgccccgcc gtggcggacg
     2281 atcatgtccg cctgcggcct cgagcacggc agaaggttcc cgcggccgga tctcctcctc
     2341 gctccgctca gcaccgcgga ctccgaggac cagcgagtgc atacccgcct gctcggagag
     2401 ggaacgtctg ctgacgtggt cgcatgggag ccgcacgcct ccccgtgaat cgttgcgaca
     2461 cgcccgggtt gcgtgaaccc catcgagccg gtatcgtctc agagtcctgt ccgccgtgtc
     2521 tggaagtcct ttgatctgaa tcgtcgcctc tccgcgtaaa tcatccacgc gctgatccga
     2581 cgatccttcc tgccaccggc ccttcgacaa gctcagggcc ccttaggcac acgcccacga
     2641 cctgcacggt cgactcgtcg gcatccgcgc acccgcacac cttccgggag ccgctcatgt
     2701 caacaccgac cactttcacc tccgccgccg tcacgctcga ccggctcacg ttcgcctggg
     2761 cggacggtcc cgtcgcgctc gacgccgtgt cgggctcgtt cggctccggc cgcaccgggc
     2821 tcgtcgggcg caacggcgcg ggcaagtcca cgctgctgcg cctgatcgcc ggtgacctcg
     2881 cccccac
//
LOCUS       DATMSX010000045         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_47952,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000045 DATMSX010000000
VERSION     DATMSX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47952"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN041_01405"
     CDS             complement(<1..207)
                     /locus_tag="VN041_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HWU27735.1"
                     /translation="MTTTLTEYFAPQPTPSAALPDPVPLLRSLTNGALEVLAGIRKVD
                     QLARWLSEDAFRALATRANLSARAR"
     gene            365..961
                     /locus_tag="VN041_01410"
     CDS             365..961
                     /locus_tag="VN041_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27736.1"
                     /translation="MEWDHLFADLEGQLAAEWEAERAALDAESERLRISKLTLRDRLR
                     AVADERTRMVLEPADGERWECTVHTIGADWIGVVLTSDPRLRILPVAAISAIGVDHGA
                     LLSSLGTDAASSAPTMRERMTLGFVLRDLARRRTPVTVARRGTDPQHGTIDRAGVDHL
                     DLAVHDEVRRARSVRGFRMIPFDSLVWIRVESGETAAS"
     gene            complement(1014..1292)
                     /locus_tag="VN041_01415"
     CDS             complement(1014..1292)
                     /locus_tag="VN041_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU27737.1"
                     /translation="MPEASDMAPRFLAPAQVAELLSIEVDEVIDLVQQGRLRGSQLGS
                     PPRWRVEEASLADYLEEQSEDARRQALWRQANEASFPELWGTTPYRRN"
     gene            1390..2004
                     /locus_tag="VN041_01420"
     CDS             1390..2004
                     /locus_tag="VN041_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HWU27738.1"
                     /translation="MMSSRAHRPFFGDLRFLIGLVLVAVSIAGVWMLVSSSRQTTPVL
                     QATRTVLPGESVSAADFQVVDVGLGGVVDAYLAPQELKSGAVATRTLPKGELVPSASI
                     GDREASRTTTIVVSSTAVPAGVEAGSAVELWQAPLKDDGRTRDAPRVLVGDAVIGRVA
                     EAEGMLSESRTDVEVVIDRADVAEVLAAITGGSIISIIPAGAGS"
     gene            2001..>2234
                     /locus_tag="VN041_01425"
     CDS             2001..>2234
                     /locus_tag="VN041_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27739.1"
                     /translation="MTSILLCAPDAEASMLASDLELEGVDVRRLDASELTAAALDGAD
                     ALLIAPTRSTLTAELIGACDRAGVRVLPVGEADT"
BASE COUNT          305 a          763 c          802 g          364 t
ORIGIN      
        1 gcgcgcacgc gccgagaggt tcgcccgtgt ggccagcgcg cggaacgcgt cctcgctgag
       61 ccagcgagcc agctggtcga ccttgcggat gccggcgagc acttccagcg cgccgttcgt
      121 caggctgcgc agcaacggga caggatcggg aagcgcggcg ctcggagtcg gctgcggtgc
      181 gaagtactcg gtcaacgtcg tcgtcatggt tccgcgatcc ccccgggtcg tggcagtcgt
      241 gcttgctgaa atatcggctc gaactccttg cagtacagca cgcgggacgg cctcgcggaa
      301 ggcgcgggcg ccgatctgtg gataactcgc acgcgcccgt cgcgcgtcgc ctacgctcgt
      361 tcgggtggaa tgggatcacc tcttcgccga cctggagggt cagctcgccg ctgagtggga
      421 ggcggagcgc gccgcactgg atgccgagtc ggagcgtctg cggatctcga agctgacgct
      481 gcgcgaccgg ctccgcgccg tcgccgacga gcgcacgcgg atggtgctgg aaccggccga
      541 cggcgagcgc tgggagtgca cggtgcacac catcggcgcc gactggatcg gggtcgtgct
      601 cacctccgac ccacggctgc gcatcctgcc tgtcgcggcg atctcggcga tcggcgtcga
      661 tcacggcgcg ctgctgtcct cgctcggcac ggatgccgcg tcatccgcgc ccacgatgcg
      721 agagcggatg acgctcggct tcgtgctgcg cgacctcgct cggcgacgca ctccggtgac
      781 ggtggcccga cgcggtaccg atccgcagca cggcacgatc gaccgcgccg gcgtcgatca
      841 cctcgacctc gcggtgcatg acgaggtccg ccgcgcgcga tcggtgcgcg gtttccggat
      901 gatccccttc gactcgctgg tctggatcag agtggagagc ggcgagacgg cggcgtcatg
      961 agagatcacg tgccagatct cgacgcctca cgcgtggcgc ggcgcggatg cggtcaattc
     1021 ctgcggtacg gagtggtgcc ccagagctcg gggaagctcg cctcgttggc ctggcgccac
     1081 agcgcctgac gtctggcatc ctcggactgc tcctcgaggt agtcggcgag gctggcctcc
     1141 tcgacccgcc agcgcggcgg cgaacccagc tgcgagcccc gcagacgccc ctgctgcacg
     1201 agatcgatga cctcatcgac ctcgatgctc agcagctcgg cgacctgcgc gggtgcgagg
     1261 aagcgcggag ccatgtcgga tgcttcgggc atgcgttcat tctcccctgc ccgggcggct
     1321 cacgcccctg agaatcggcc ctgtggataa cttttcgggc accttcatcg gatctgtgac
     1381 gatgtgtgca tgatgtcctc gcgtgcacac cgcccgttct tcggcgatct ccggttcctg
     1441 atcggcctcg tgctggtcgc ggtctccatc gccggggtat ggatgctggt gtcctcctcg
     1501 cgtcagacga cgccggtgct ccaggccacg cgcaccgtcc tgcccggtga gagcgtcagc
     1561 gccgcagact tccaggtcgt cgatgtgggt ctgggcgggg tcgtcgacgc atatctcgct
     1621 ccgcaggagc tgaagtcggg tgcggtcgcg acgcgcaccc tgccgaaggg cgagctggtg
     1681 ccctcggcga gcatcggcga ccgggaggcg tctcgcacga cgaccatcgt ggtcagcagc
     1741 acggccgttc ccgccggcgt ggaggcgggc agcgcggtcg agctctggca ggcgccgctg
     1801 aaggatgacg gccgcactcg cgacgcccct cgtgtgctcg tcggcgatgc ggtcatcggc
     1861 agggtcgccg aggccgaggg catgctcagc gagagccgca ccgacgtgga ggtggtgatc
     1921 gaccgcgcgg acgtcgcaga ggtgctggcg gcgatcaccg gcggatccat catctcgatc
     1981 atccccgcgg gagcgggctc gtgaccagca tcctgctgtg cgcgccggac gccgaggcct
     2041 cgatgctggc ctccgatctg gagctcgagg gcgtcgacgt gcgtcgtctg gacgcgtccg
     2101 agttgacggc cgccgcgctg gacggagcgg atgccctgct gatcgcgccc acacggtcga
     2161 cactgaccgc cgagctgatc ggcgcctgcg atcgcgcggg cgtgcgcgtt ctgcccgtcg
     2221 gcgaggccga cacc
//
LOCUS       DATMSX010000046         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123894,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000046 DATMSX010000000
VERSION     DATMSX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123894"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VN041_01430"
     CDS             complement(<1..644)
                     /locus_tag="VN041_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWU27740.1"
                     /translation="MPGLLTSVDHALADSGMAGALVIPHAVVIVAAVVFGVIAVALAW
                     PVPVILSRATWPMRAPVMALLLWQVIGLAGGLSMIGALALAGYAALPQHPWLAPIPAV
                     LFTVYLLTHLAITVVQVTRLRHRHLALLEMLTSPHPTRARTRVLEDAVPVAYCLPRGA
                     RSVTVLSQGLLDRLDADELVAVIAHERAHVEQRHDLLLLAFRAWRSALPWFPIAA"
     gene            complement(655..1011)
                     /locus_tag="VN041_01435"
     CDS             complement(655..1011)
                     /locus_tag="VN041_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWU27741.1"
                     /translation="MSTLGELERAVMDALWDADRSLSAYDVQHTLEAEGRTLAATTLL
                     TVLSRLEKKGFVASDRSSRPHRYTAVASRADHVAELMHSVLGDAGDRTAVLERFVGGV
                     SAEDAAVLRRLLGGAG"
     gene            1155..2735
                     /locus_tag="VN041_01440"
     CDS             1155..2735
                     /locus_tag="VN041_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWU27742.1"
                     /translation="MDVLDIARWQFGITTVYHFLMVPLTLGLGMMVAIMQSRWVRTGD
                     EKLLRMTKFWGKVYLINFIVGVATGLVQEFQFGMAWSEYSRFVGDVFGAPLAMEGLLA
                     FFVESTFLGIWIFGWGRLRKGLHLAAIWCAVIGSWISAFFIIVANSWMQHPVGVEMGA
                     DGRPVMTDVWAVLTNNTALAAYTHTILGALVVAGMFLLGISWYHLWRRRHDGIDTVDE
                     AGRVVVGETDAAPGRDRTDHGVWIWSLRLGAVVAVLGFLGTVVSGDVQGKLMYEQQPM
                     KMAAAEAACHTGSDFSVLSLGDPGSKDCGDVITLIAVPGALGFLGTGEWGADMPGIRD
                     LETHYVDQYGKTIPDEAIYGDLAGSDVQYVPVMWVTYWGFRLMIGLGGVVALGGLVAL
                     WLTRKGTVPRSPWIMRLALLGIVAPFAANIAGWIFTELGRQPFVVAPNPTGVDGVYMF
                     TAAAVSPGVTAGELLFSVITLSAVYGIMLVIELVLMVKYIRGGVAASMPELVARASDS
                     DDDHPSGDHRDDVLAFAY"
     gene            2757..>3361
                     /gene="cydB"
                     /locus_tag="VN041_01445"
     CDS             2757..>3361
                     /gene="cydB"
                     /locus_tag="VN041_01445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWU27743.1"
                     /translation="MEPLPVVWFIAIAVLWTGYLLLEGFDLGVGMHMIFSTRSERDRR
                     VMLNTIGPVWDGNEVWLITAGAAMFAAFPAWYAALFSTLYVPLTITLVGLIIRAVGIE
                     YRGKLHTERWRTLWTWMIGLGSLIVAFCVGAALALTSTGLPLDANGDRVGGPFVWLTL
                     PAVLGGLAVVGFALLHSATFLGLKADGPVRERAGRFAGRWAP"
BASE COUNT          495 a         1132 c         1172 g          562 t
ORIGIN      
        1 gccgcgatcg ggaaccaggg cagcgccgag cgccaggccc ggaacgccag cagcagcagg
       61 tcgtggcgct gctccacgtg ggcgcgttcg tgcgcgatga ccgcgacgag ctcatccgca
      121 tcgagacgat ccagcaggcc ctgcgacagc accgtcaccg agcgtgcgcc gcgcggcagg
      181 cagtacgcga cgggcacggc gtcctccagc acccgggtgc gcgcgcgggt ggggtgcggg
      241 gaggtgagca tctcgagcag cgccaggtgg cggtggcgca gccgcgtcac ctgcacgacc
      301 gtgatcgcga gatgggtcag caggtacacg gtgaacagga cggcggggat cggcgcgagc
      361 cacggatgct ggggaagggc cgcgtagccg gccagtgcga gcgcgccgat catcgacagc
      421 cctccggcca ggccgatcac ctgccagagc agcagggcca tcaccggtgc gcgcatgggc
      481 cacgtggcgc gggagaggat gaccggcacc ggccacgcca gtgcgacggc gatcacgccg
      541 aagacgacag cggcgacgat gacgaccgca tgcgggatca ccagcgcccc ggccatgccg
      601 gaatccgcca gcgcgtggtc gaccgaggtc agcaggccag gcatggccgt gtgctcagcc
      661 cgcgccgccg agcagacggc gcagtacggc ggcatcctcc gccgacacac cgcccacgaa
      721 gcgctcgagc accgcggtgc ggtcgcccgc atcgcccagc acggagtgca tcagctcggc
      781 cacgtgatcg gctcgcgacg cgaccgcggt gtagcgatgc ggtcgcgagg accggtccga
      841 ggcgacgaag cccttcttct ccagcctcga cagcacggtc agcagtgtcg tcgcggccag
      901 ggtccggccc tcggcctcca gggtgtgctg cacgtcgtag gcgctcaggg agcggtccgc
      961 gtcccagagt gcgtccatga cggcgcgttc cagttctccg agcgtgctca tcggcgcctc
     1021 ccgtgcttgt ggtttcttcc ggcgcatcca ttctacctct cgtcgaaatt gttctattgt
     1081 gtgtcgaagt agttctacaa ggcgtagaat tcaagaggat cggacgcacg gaacgatccg
     1141 aggaggagac ccgcatggac gtgctcgaca tcgcccgctg gcagttcggc atcacgaccg
     1201 tctaccactt cctgatggtg ccgctgacac tgggcctggg catgatggtc gcgatcatgc
     1261 agtcccgctg ggtgcgcacg ggagacgaga agctcctgcg catgacgaag ttctggggga
     1321 aggtctacct gatcaacttc atcgtcggcg tggcgaccgg cctggtgcag gagttccagt
     1381 tcggcatggc ctggagcgag tactcccgct tcgtcggtga cgtcttcggc gccccgctcg
     1441 cgatggaggg cctgctcgcc ttcttcgtcg agtcgacctt cctgggaatc tggatcttcg
     1501 gctggggtcg cctgcgcaag ggcctgcacc tcgcggcgat ctggtgcgcg gtgatcggat
     1561 cgtggatctc ggcgttcttc atcatcgtcg cgaactcctg gatgcagcat cccgtcggcg
     1621 tggagatggg agccgacgga cggccggtca tgaccgacgt gtgggccgtg ctcaccaaca
     1681 acaccgccct cgccgcctac acgcacacga tcctcggtgc gctggtcgtt gccggcatgt
     1741 tcctgctcgg catctcgtgg taccacctgt ggcgccgtcg ccacgacggc atcgacacgg
     1801 tcgacgaggc gggccgcgtg gtcgtggggg agacggatgc cgcgcccggg cgcgaccgca
     1861 ccgaccacgg cgtctggatc tggtcgctgc ggctgggcgc cgtcgtcgcc gtcctcggct
     1921 tcctcgggac ggtcgtctcg ggcgatgtcc agggcaagct catgtacgag cagcagccca
     1981 tgaagatggc cgccgcggaa gccgcatgcc acaccggctc ggacttctcg gtgctctcgc
     2041 tcggcgaccc tggttcgaag gactgcggcg acgtcatcac cctgatcgcg gtcccggggg
     2101 cactgggctt cctgggcacg ggggagtggg gtgccgacat gcccggcatc cgcgacctcg
     2161 agacgcacta cgtcgaccag tacggcaaga ccatcccgga tgaggcgatc tacggcgacc
     2221 tggccggcag tgacgtgcag tacgtgccgg tcatgtgggt cacctactgg ggcttccggc
     2281 tcatgatcgg cctgggcggc gtcgtggcgc tcgggggcct ggtcgccctg tggctgaccc
     2341 gcaagggcac ggtgccgcgc tcgccgtgga tcatgcgcct ggcgctgctg ggcatcgtcg
     2401 ccccgttcgc ggcgaacatc gccggctgga tcttcaccga gctgggacgg cagccgttcg
     2461 tcgtggcacc caaccccacc ggagtcgacg gggtgtacat gttcacggcc gcggctgtct
     2521 caccgggagt cacggcaggg gagctgctgt tctcggtgat cacgctcagc gcggtctacg
     2581 ggatcatgct cgtgatcgag ctggtgctga tggtcaaata catccgcggt ggggtcgcgg
     2641 catccatgcc cgaactcgtt gcacgggcat ccgactccga cgacgaccac ccgtccggtg
     2701 accaccgcga cgacgtgctc gcgttcgcgt actgagcgct gagaggaaca acgaaaatgg
     2761 aaccgcttcc cgtcgtctgg ttcatcgcga tcgccgtgct ctggaccggc tacctgctgc
     2821 tcgagggctt cgacctcggt gtcggcatgc acatgatctt ctccacccgc tccgagcgcg
     2881 atcgtcgcgt catgctcaac accatcgggc ccgtgtggga cggcaacgag gtgtggctga
     2941 tcaccgccgg tgctgcgatg ttcgcggcct tccccgcctg gtacgcggca ctgttctcga
     3001 ccctctacgt ccctctgacg atcacgctgg tcgggctgat catccgggcc gtgggcatcg
     3061 agtaccgcgg caagctgcac accgagcgct ggcgcacgct gtggacctgg atgatcggtc
     3121 tcggctcgct gatcgtcgcg ttctgcgtgg gtgcggccct ggcgctgacc tcgaccggcc
     3181 tgccgctgga tgccaacggc gaccgcgtcg gcggaccgtt cgtctggctg accctgccgg
     3241 cggtgctggg tggtctcgcc gtcgtcgggt tcgcgctgct gcactccgcc accttcctcg
     3301 ggctcaaggc ggacgggccg gtgcgcgagc gcgccggacg cttcgccgga cgatgggcgc
     3361 c
//
LOCUS       DATMSX010000047         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_114301,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000047 DATMSX010000000
VERSION     DATMSX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114301"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..559
                     /locus_tag="VN041_01450"
     CDS             245..559
                     /locus_tag="VN041_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27744.1"
                     /translation="MDLVMGVALLLLQAAASWVSLYVFILQALSIAGCGDRCDYALAD
                     ASHRTQLWGSLLLLIAAAVAVVWLWLCRKPTWRAPVIGIGAVVLLTIATCIGMDIATP
                     TL"
     gene            701..1204
                     /locus_tag="VN041_01455"
     CDS             701..1204
                     /locus_tag="VN041_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU27745.1"
                     /translation="MARNARVMHCTPEDVFEVLADGWLYPSWVVGASRMRDVDENWPR
                     PGARLHHSVGVWPMLINDETVAEEFDSPRAMVMRATGWPLGEARVTLHVKPRGSHTIV
                     RMQEEAIAGPARYVPRAIMDIMLQWRNAETLRRLAFLAEGRSSRRAGEPVTPSAIPAD
                     ESEMPAS"
     gene            1201..>1804
                     /locus_tag="VN041_01460"
     CDS             1201..>1804
                     /locus_tag="VN041_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU27746.1"
                     /translation="MSTHSGDAGLDAIVVGAGPNGLAAAVTLARAGLRVRVYERANQP
                     GGGASTRELTLPGFHHDVCSAVHPMAFESRFFREFGLAGRVEFITPEVSFAQPLYGGR
                     AAVAYRDLERTSTELRADGPAYDRLMRPLVERSTEVADFTGSSLLRIPRNPLTAVAFG
                     LRALEQGTPAWGIRFREDLAPALLTGVAAHTILAQPSIAGA"
BASE COUNT          265 a          634 c          633 g          272 t
ORIGIN      
        1 ggcgccggca tccaccgcgc ccgcgttcac ccccggcgca cccgcgccgt ctgagcgcgc
       61 ctgcgaccgc aggcgttccg cgagttcgcg atgcgaggca tccgctggca tcccccgatg
      121 ctactcccgg agtcaatcca cccggcggat aagctctgac gaaacccgga tccggcgagg
      181 ggaacggcag atgcatgtga cgcgaccgcc acgcaccgag gagtcacagc cccgaaggga
      241 cctgatggat ctcgtcatgg gcgtcgcgct gctgctcctc caggccgctg cctcgtgggt
      301 gtcgctctac gtgttcatac tgcaggcgct cagcatcgca ggctgcggcg accgctgcga
      361 ctacgcgctc gcggatgcct cgcatcggac gcagctctgg ggaagcctcc tgcttctcat
      421 cgcggcagcc gtcgctgtcg tctggctctg gctgtgcagg aagccgacct ggcgggcgcc
      481 cgtgatcggc atcggcgcgg tcgtgctgct gacgatcgcg acctgcatcg gcatggacat
      541 cgcaacgccg acgctctgaa tcatggtccg aggcgggggc atccggcggc ggcaggccac
      601 gtgccatacg gcgctcgagt gcgcaacccg ctgtcgaccg gcgacggcgg cgattacgtt
      661 cagggcgacc ggcatcggcg acgagacgag gagatgcgag atggcccgca atgcgagggt
      721 gatgcactgc acaccggagg acgtgttcga ggtgctggcc gacggctggc tgtatccgtc
      781 gtgggtggtc ggggcatccc gcatgcggga cgtggatgag aactggccgc gacccggtgc
      841 gcgtctgcac cactccgttg gggtgtggcc gatgctcatc aacgacgaga ccgtggcgga
      901 ggagttcgac tctccgcgag ccatggtgat gcgcgcgaca ggctggccac tcggtgaggc
      961 ccgggtcacg ctgcacgtca aaccccgcgg gtcccatacg atcgtgcgca tgcaggagga
     1021 agcgatcgcg ggtcccgcga gatacgtgcc acgcgcgatc atggacatca tgctgcagtg
     1081 gcgcaatgcc gagaccctgc gccggctcgc cttcctcgcc gaggggcgct catcacggcg
     1141 tgcaggtgag ccggtgacgc cctccgcgat tcccgccgac gaatcggaga tgcccgcatc
     1201 gtgagcacgc acagcggtga cgccgggctg gacgcgatcg tcgtcggtgc cggaccgaac
     1261 ggccttgccg ccgccgtgac gctcgcccgc gcaggtctgc gcgtgcgtgt ctacgagcgc
     1321 gcgaatcagc ccggcggcgg cgccagcacg cgcgagctga cgctgccggg gttccaccac
     1381 gacgtgtgct cggcggtgca tccgatggcc ttcgagtcac ggttcttccg cgagttcggg
     1441 ctggccggca gggtcgagtt catcacgccg gaggtgtcgt tcgcgcagcc gctctacggc
     1501 gggcgcgccg cggtcgccta ccgcgatctc gagcgcacca gcaccgaact cagggcggac
     1561 ggacccgcgt acgaccggct gatgcggccg ctggtcgagc gctccaccga ggtcgccgac
     1621 ttcaccggat cgtcgctgct gcgcatcccg cgcaacccgc tcaccgccgt cgccttcggg
     1681 ctgcgagcac tcgagcaggg cacgcccgca tggggcatcc gcttccgcga ggacctggcg
     1741 cccgcccttc tcaccggagt cgccgcgcac acgatcctgg cgcagccgag catcgccggg
     1801 gcgg
//
LOCUS       DATMSX010000048         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_133291,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000048 DATMSX010000000
VERSION     DATMSX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133291"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VN041_01465"
     CDS             <1..472
                     /locus_tag="VN041_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWU27747.1"
                     /translation="PKRRRFAYKPQLLLVDGGQPQVNAAARALRDSGRTDIALAGIAK
                     RLEELWLPDDDFPVILPRTSEALYLVQRLRDEAHRFAITHQRRRRRRDITTILSEIPG
                     LGESRIKALLKHFGSVTALRAATPEQIREVNGIGPALADGIHEHLSGRPAAPSR"
     gene            501..1373
                     /gene="rapZ"
                     /locus_tag="VN041_01470"
     CDS             501..1373
                     /gene="rapZ"
                     /locus_tag="VN041_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWU27748.1"
                     /translation="MTAADEGEFLIVTGMSGAGRSTAANALEDLGWYVVDNLPPQILR
                     PLLELTDMGGANLPKIAAVVDVRGRNLFDDFPGVARALRDRGGVKVLFLDASDDVLVR
                     RFESVRRPHPLQGDGTLLDGIRTERSRLAPIREAADVLIDTSSLNIHQLATQVSEMFS
                     EEGAARHRLTFLSFGFKYGVPTDVDLVADMRFLPNPFWNEELRDHTGQDAEVRDYVLS
                     RPGAEEFLAAYVQALRPVLEGYQRENKSHSTVAIGCTGGKHRSVAMAEELARRIADAP
                     GVAVNVRHRDLGRE"
     gene            1460..>1939
                     /gene="whiA"
                     /locus_tag="VN041_01475"
     CDS             1460..>1939
                     /gene="whiA"
                     /locus_tag="VN041_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HWU27749.1"
                     /translation="MALTTDVKAELVSIRNAPPTVRVAEVTTILRFAGGLHSIAGRVA
                     VEAEVDAELLARRVSRDLAEIYGVRPEIAQVKASTAQSGGDGARWAVRVIGPGETLAR
                     QTGLLDARRRPVRGLPNRLTTGSPEELAGIWRGAFLAAGSLSEPGRSAMLEVACPTGE
                     "
BASE COUNT          288 a          691 c          679 g          281 t
ORIGIN      
        1 acccaagcgc cggcgcttcg cctacaagcc gcagctgctc ctggtcgacg gcgggcagcc
       61 gcaggtgaac gccgccgcac gcgccctgcg cgacagcgga agaaccgaca tcgccctggc
      121 gggcatcgcc aagcgcctcg aggagctgtg gctcccggac gacgacttcc cagtgatcct
      181 gccccgcacc agtgaggccc tctacctcgt gcagcgcctg cgcgacgagg cccaccgctt
      241 cgcgatcacg catcagcggc gccggcggcg gcgcgacatc accacgatcc tcagcgagat
      301 ccccggactc ggcgagtcgc gcatcaaggc gctgctcaag cacttcgggt cggtcacggc
      361 gctgcgggcg gcgacgccgg agcagatccg cgaggtgaac ggcatcgggc ccgccctcgc
      421 cgacggcatc catgagcatc tctccggacg tccggccgcc ccgagtcgat aggctgggtc
      481 gcaggggaag ggagacgacg atgacggccg cagatgaggg cgagttcctg atcgtcacgg
      541 gcatgtcggg cgcagggcgc tcgacggccg cgaacgcgct cgaggacctc ggctggtacg
      601 tcgtcgacaa cctgccgccg cagatcctcc ggccgctgct ggagctcacc gacatgggcg
      661 gtgccaacct gcccaagatc gctgcggtgg tggatgtgcg cggacgcaac ctgttcgacg
      721 acttccccgg ggtcgccagg gcgctgcgcg atcgtggcgg tgtcaaggtg ctcttcctgg
      781 acgcctccga cgacgtgctg gtgcgccgtt tcgagtcggt ccggcggccg catccgctgc
      841 agggcgacgg cacgctgctg gacggcatcc gcaccgagcg cagccgcctg gcgccgatcc
      901 gcgaggccgc cgacgtgctc atcgacacct cgagcctgaa catccatcag ctggccacgc
      961 aggtctccga gatgttctcc gaggagggcg cagcgcgcca ccgcctgacg ttcctgagct
     1021 tcggcttcaa gtacggcgtc ccgacggatg tcgatctcgt cgcggacatg cgcttcctgc
     1081 ccaatccgtt ctggaacgag gagctgcgcg accataccgg tcaggatgcc gaggtgcgcg
     1141 actacgtgct gtcccggccc ggtgcagagg agttcctcgc cgcctacgta caggcgctgc
     1201 gtcctgtgct ggagggctat cagcgcgaga acaagagcca ctccaccgtg gccatcggat
     1261 gcacgggcgg caagcaccgc tcggtggcca tggcggagga actggcccgt cgtatcgcgg
     1321 atgcgcccgg ggtggcggtc aacgtgcggc accgggatct cgggcgcgaa tgatgcgctc
     1381 cgagtcggtg ggagcgcccg cggccttgta ggctagtccc ttgcgtcgcg atccgacgcc
     1441 aggatcgtga aggagtcccg tggcactaac caccgacgtc aaggccgagc tcgtcagcat
     1501 ccgcaatgcg cccccgacgg tgcgcgtcgc agaggtgacg acgatccttc ggttcgccgg
     1561 tggactgcac tcgatcgccg gtcgggtggc cgtcgaggcc gaggtggacg ccgagctgct
     1621 ggcgcgccgc gtgagtcgcg acctcgccga gatctacggt gtccgccccg agatcgccca
     1681 ggtgaaggcc agcaccgcgc agagcggcgg cgacggcgcc cgctgggccg tgcgcgtgat
     1741 cggcccgggg gagaccctcg cccgtcagac cggtctgctg gatgcccgtc gtcgtcccgt
     1801 gcgcggtctt ccgaaccgcc tgaccaccgg atcccccgag gagctcgcgg gcatctggcg
     1861 cggcgccttc ctcgcggccg gatccctcag cgagccgggc cgctccgcga tgctcgaggt
     1921 cgcctgcccg accggcgag
//
LOCUS       DATMSX010000049         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_171285,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000049 DATMSX010000000
VERSION     DATMSX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171285"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..482
                     /locus_tag="VN041_01480"
     CDS             30..482
                     /locus_tag="VN041_01480"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27750.1"
                     /translation="MTSRGRASRATYSAGSTTTEVARQLGIRKSPLGVDVVLDGRQVL
                     SSASEEQLLAEVALGPAQAVVTVIGGQGFLLGRGNQQLSASVIRALGDDPLVVVAPEQ
                     KLIDLHGRPLIVETGDAELDARLAGHIRIVTGPGTSSLYAVAAPDLAL"
     gene            514..1272
                     /locus_tag="VN041_01485"
     CDS             514..1272
                     /locus_tag="VN041_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU27751.1"
                     /translation="MRLENKKAIVTGGAGGIGRATSLAFAAEGASVAVVDLNAEAAEA
                     VAEEIRAAGGNAIAISADVSSEDDIIRVVATANEAFEGIDVVFNNAGIIRRTTAVETT
                     VEEWDRVFGVNVRSIFLMCKHVVPVMKANGGGSIVNTGSGWGLKGGGQAISYCASKGA
                     VVNMTRALAIDHGPDGIRVNSVNPGDVDTGMLRDEAKQLGQDQAGFLAEAAERPLNRM
                     GQPSEIAAAVVWLASDDASYVTGSALVVDGGGIA"
     gene            1437..1868
                     /locus_tag="VN041_01490"
     CDS             1437..1868
                     /locus_tag="VN041_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HWU27752.1"
                     /translation="MTVPEINYWAVLIATVSSMIVGAIWYARGVMGTRWAALAHVDLN
                     RPSRGPLWPMITTVIVSFITAWVLAGAATIAQNFYTGSYFWASLVTAVTLWAGFTAAR
                     FITHDAFEGRSTKLTTLNIAHELVTVVVMAIIIGVWPPAGI"
     gene            complement(2050..2757)
                     /gene="purQ"
                     /locus_tag="VN041_01495"
     CDS             complement(2050..2757)
                     /gene="purQ"
                     /locus_tag="VN041_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWU27753.1"
                     /translation="MTARIGVITFPGSLDDRDAQRAVRIAGAEPVALWHGSHDLEGVD
                     ALVLPGGFSYGDYLRSGAIAALSPIMAEVKDAAAKGMPILGICNGFQMLVEAHLLPGG
                     LIRNDHQHFVRRDQKLTVENATTAWTSEFTQGQEIVIPLKNGEGGYIAADDTLDRLEG
                     EGLVAFRYAGVNPNGSLRDIAGLTNERGNVVGLMPHPEHATEPGFGPDTADAMRSGVD
                     GLGFFTSAIAAVAAVAA"
     gene            complement(2754..3002)
                     /gene="purS"
                     /locus_tag="VN041_01500"
     CDS             complement(2754..3002)
                     /gene="purS"
                     /locus_tag="VN041_01500"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWU27754.1"
                     /translation="MPTIVVDVMPKAELLDPQGKAVSGAFARLGVEGFSQVRIGKRFE
                     LTVDGEVTDELLAEARRVAEDVLSNSVIEDVVGVEVAQ"
     gene            3306..>3863
                     /locus_tag="VN041_01505"
     CDS             3306..>3863
                     /locus_tag="VN041_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU27755.1"
                     /translation="MDVKLSRLAATSGVSTTTIKHWIRVGIMPAGRLKNATTAVYEQR
                     HVDRARLILVMRDVYGASIAAIRSLTELIDTPGILTLDVMNACQAFALGVPEGIADDP
                     AYAEFRERTYELMRRRDWRGYPGAAERGLTFALAQASEVGLDYDVEELMEYADALEPL
                     AGRNVAALGPEGSADEVARRMLLAVN"
BASE COUNT          579 a         1333 c         1391 g          560 t
ORIGIN      
        1 ccggcagccg gtgccggtcc ggcttgccgt tgacgtccag gggcagggcg tccagggcca
       61 cgtactcggc gggcagcacg acgacggagg tcgcccggca gctcggcatc cgcaagtccc
      121 cgctgggcgt ggacgtggtg ctcgacgggc ggcaggtgct ctcctccgcg agcgaggagc
      181 agctgctcgc cgaggtcgcg ctggggcccg cgcaggccgt ggtcaccgtg atcggcgggc
      241 aggggttcct gctcgggcgc ggcaaccagc agctctcggc atccgtcatc cgcgccctcg
      301 gcgacgaccc gctggtggtc gtcgcccccg aacagaagct catcgacctg cacggccggc
      361 cgctgatcgt cgagaccggt gacgccgagc tcgacgcccg cctggccggg cacatccgca
      421 tcgtcaccgg gcccggcacg agcagcctgt acgctgtcgc cgcgcccgac ctcgccctct
      481 gaaccacaac ccgactccct ggaaggaccc atcatgcgtc tcgagaacaa gaaggccatc
      541 gtcaccggcg gtgcgggcgg catcggccgc gccacctccc tcgccttcgc ggcggagggc
      601 gcctcggtcg ccgtcgtcga cctgaacgcc gaggccgccg aggcggtggc cgaggagatc
      661 cgtgcggcgg gcggcaacgc catcgcgatc agcgccgacg tctccagcga ggacgacatc
      721 atccgcgtgg tcgccaccgc gaacgaggcg ttcgagggca tcgacgtggt cttcaacaac
      781 gcgggcatca tccgccgcac caccgccgtc gagaccacgg tcgaggagtg ggaccgggtg
      841 ttcggcgtga acgtgcgctc catcttcctg atgtgcaagc acgtcgtgcc ggtgatgaag
      901 gcgaacggcg gcggctccat cgtgaacacg ggctccgggt gggggctcaa gggcggcggt
      961 caggcgatct cttactgcgc ctccaagggc gcggtcgtga acatgacccg tgcgctggcg
     1021 atcgaccacg gtcccgacgg catccgcgtc aactcggtca accccgggga cgtcgacacc
     1081 gggatgctgc gcgacgaggc caagcagctc ggccaggacc aggcgggctt cctcgccgag
     1141 gccgccgagc gcccgctgaa ccggatgggc cagccgagcg agatcgccgc tgccgtggtg
     1201 tggctcgcga gcgacgacgc gtcgtacgtg accggctccg cgctcgtcgt ggacggcggc
     1261 ggcatcgcct gacgcgcgct cggtcggcat ccgccctgtt gcactccggg ggccgacacg
     1321 ccagatctcg gacgtttccc cgcgaaaccg tccggcgaat ggcgtgtcgg cccccgagct
     1381 gtatcgccgg cgagcggcgg cgagtagcct gagcgcatcg gagcgaggag gctgccatga
     1441 cggttcccga gatcaactac tgggctgtgc tgatcgccac ggtgtcgagc atgatcgtgg
     1501 gcgcgatctg gtacgcgcga ggcgtgatgg gcacgcgctg ggcggcactc gcgcacgtcg
     1561 acctgaacag gccgtcgcgc ggaccgctgt ggccgatgat cacgacggtg atcgtgagct
     1621 tcatcaccgc gtgggtgctg gccggcgccg ccaccatcgc ccagaacttc tacaccggct
     1681 cgtacttctg ggcctcgctc gtcacggcgg tcacgctgtg ggccggattc accgcggccc
     1741 gcttcatcac gcacgacgcc ttcgagggcc gctcgacgaa gctgaccacg ctgaacatcg
     1801 cgcacgagct cgtgacggtc gtcgtgatgg cgatcatcat cggcgtctgg ccgcccgccg
     1861 gcatctgacg aagtcacgtc gcgaggacgg ggcggccggc cgtgtcccag aaggtgcggg
     1921 tacggatgcc ggggacccgc actttctgcg acaagcggcg gcccgcgtct gctaacggac
     1981 ggtgcgcgaa aatgcgaaag gcggatgccg ggcgcgggcc cggcatccgc ctgaaagtgt
     2041 gagcgagcgt tacgctgcga cggccgccac ggcggcgatc gcgctggtga agaaccccag
     2101 gccgtcgacg ccggagcgca tggcatctgc ggtgtcggga ccgaagccgg gctcggtggc
     2161 gtgctcgggg tgcggcatga ggccgaccac gttgccgcgc tcgttcgtga ggccggcgat
     2221 gtcgcgcagc gacccgttcg ggttcacgcc cgcgtagcgg aacgccacca ggccctcgcc
     2281 ctcgaggcgg tcgagcgtgt catccgccgc gatgtagccg ccctcgccgt tcttcagcgg
     2341 gatgacgatc tcctggccct gcgtgaactc gctggtccat gcagtcgtgg cgttctcgac
     2401 cgtgagcttc tggtcgcggc gcacgaagtg ctggtggtcg ttgcggatca ggccgccggg
     2461 cagcaggtgc gcctcgacga gcatctggaa gccgttgcag atgccgagga tcggcatgcc
     2521 cttggcggcg gcgtccttga cctcggccat gatcggcgag agcgcggcga tcgcgccgga
     2581 gcgcaggtag tcgccgtagc tgaagccgcc gggcaggacg agggcgtcca cgccctcgag
     2641 gtcgtgcgag ccgtgccaga gggcgacggg ctcggcgccg gcgatgcgca ccgcgcgctg
     2701 cgcgtcccga tcgtcgagcg aaccggggaa ggtgatgacc ccgatgcgtg cggtcactgc
     2761 gcgacctcga cgcccacgac gtcctcgatc acggagttcg acagcacgtc ctcggccacg
     2821 cggcgggcct cggcgagcag ctcgtcggtg acctcaccgt cgacggtgag ctcgaagcgt
     2881 ttgccgatgc ggacctggct gaagccctcg acgcccagcc gggcgaaggc gccggagacg
     2941 gccttcccct gcgggtcgag cagttcggcc ttgggcatga cgtcgacgac gatggtgggc
     3001 acggctggcc tccaggaacg cgcgggcggg aacaggaaag agtctaccgc ccgccgaccg
     3061 ggctcccgcc ccgggggaga cggatgccga gtgcgacggg cggtaggagg gcccgggcgg
     3121 tttcgcaccc ctcagtgttt ccgcccgggc cgcagtcgag gtttccccag tcctcattca
     3181 gctcacgagt gaccccccaa tcgagagcag tcccttcccc agcaggttag agtcttcgca
     3241 cggcctgcgc atcgtggaga gcgcatctgc accccactct ccaagacgcg ggcgcggggg
     3301 aggggatgga tgtgaagctc agcaggctcg cggcgaccag cggcgtcagc accaccacga
     3361 tcaagcactg gatccgggtg gggatcatgc ccgccggtcg cctcaagaac gcgaccacgg
     3421 cggtgtacga gcagcgtcat gtcgatcggg cccgcctcat cctggtgatg cgcgacgtct
     3481 acggtgcgtc gatcgcggcg atccgctcgc tgaccgaact gatcgacacc ccgggcatcc
     3541 tgacgctgga tgtgatgaat gcgtgtcagg cgttcgcgct cggcgtcccg gagggcatcg
     3601 ccgacgaccc cgcgtacgcg gaattccggg agcgcaccta cgagctgatg cgccgccgcg
     3661 actggcgcgg ctatcccgga gcggccgaac gcgggctcac cttcgcgctc gcccaggcat
     3721 ccgaggtcgg tctcgactac gacgtcgagg agctgatgga gtacgccgac gcgctggagc
     3781 cgctggccgg ccgcaatgtc gcggcgctcg gcccggaggg atccgccgac gaggtggcca
     3841 ggcggatgct gctcgccgtc aac
//
LOCUS       DATMSX010000050         8745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_739, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000050 DATMSX010000000
VERSION     DATMSX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..655
                     /locus_tag="VN041_01510"
     CDS             68..655
                     /locus_tag="VN041_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase family protein"
                     /protein_id="HWU27756.1"
                     /translation="MAVRTPDPDPDGGFGFDDVPAADSNPAWLSDIELEEARRRLPML
                     YVEAVPVVTDGAGQVTSIGVLLRSTPLGEITRTLVSGRVRYGETVRDALFRHIENDLG
                     PMAFPLLPVSPTPFAVAEYFPIPGVSAFHDDRQHAVSLAFVVPVTGTCEPRQDALEVT
                     WFSPEEAASDGLSAEMEGGRGTLLRQALAHLGLLR"
     gene            702..2615
                     /locus_tag="VN041_01515"
     CDS             702..2615
                     /locus_tag="VN041_01515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWU27757.1"
                     /translation="MSEPIAFPTASADWLAFASTRTDERLARVDELAARLKDGSSRTA
                     LEALQLWNDLQIELRGAYNEAEVLAEVHPDRAVREAAETRTSAAQAKDAELMTDADLA
                     AVFGAADAEALDADAARLLGKIQRDFRRGGADRDAETRERLRAFAARETELSVTFARN
                     IREGRREIRIAPDKLAGLPEDFMAEHPVGEDGLVAVSTDNLDVMAVMNYAHDRETRSA
                     LRVARSDLAWPENDSVLAELLDVRQQHAELLGYASWADYETEDRMARSGARVASFLEE
                     VDEASKAAAEAEYAILLERLREDVPDAEGVTNADQMYLLTRLHEERFAVDAQQVRRHL
                     DFTRVLGGLLSTTGRLFGIEYAPVDVPAWHDEVRSYDVLRAGERIGRIHLDLHPREGK
                     FNHAAVFGLAPGVRGRVLPEAALVCNFPTGLMEHRQVQTFFHEFGHLVHDILGGDQEW
                     APFSGVATEWDFVEAPSQMLEEWVWDAGVLASFALDETGAPIPAELVARMREADAFGR
                     ALLVRTQLGHARVSYHLHMDRPADLAADTDHWYEVSSPLQHLRGTHSYAAFGHLTGYG
                     SCYYTYQWSLVIARDLLTGFTGLMDEASATRYRREILEPGGRRDAADLIEAFLGRPFT
                     VDAYREWLGAPAA"
     gene            complement(2730..3797)
                     /locus_tag="VN041_01520"
     CDS             complement(2730..3797)
                     /locus_tag="VN041_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HWU27758.1"
                     /translation="MSTLHDTAVRGFASDNYSGIHPEVLAAIAAANDGHQIAYGEDLY
                     TTRLQEVFARHFGEGVQAFPVFNGTGANVTGLQSMLPRWGAVISASTAHINVDEGGAP
                     EKIGGFKLLTVPSDDGKLTPELVDREAWGWGDEHRAQPLVVSITQSTELGTLYTVDEI
                     KALADHAHERGMKLHLDGARISNAAAALDVPLRAFTTDAGVDVLSFGGTKNGAMLGEA
                     VVVLNPEASDGLIYSRKYNMQLSSKMRFVSAQLIALLEGDLWLRNARHSNAMAQRLRG
                     EVEAGLADGSIQGVSFSQPTQSNGVFAVLPDGVADKLRESFRFYDWDASRNEVRWMCS
                     FDTQESDVDAFVAELGRLTTA"
     gene            3879..5201
                     /locus_tag="VN041_01525"
     CDS             3879..5201
                     /locus_tag="VN041_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27759.1"
                     /translation="MRTFLQVLVNTMVANVTTSFLWFALTFWVYLETRNVMATGIVGG
                     AYMLLIAIFSIVFGTIVDRHRKRAVFVFSSAITTVAFVLAWLIFLWQPERALLDFGGP
                     WFWLFAGIILLGAVVEQMRSIALSTTVTLLVPVERHANANGMVGTVQGLAFLVTSVFS
                     GLSVGFLGMGGTLLIALGLTVVSLVHILFIRIPEEQPQHDPDAPSAMDLRGSIRAIRA
                     VPALFALLFFSMLNNLVGGVYMALMDPYGLELMSVQMWGIALGVASTGFLIGGIVVST
                     AGLGKNPIRTMLMLVGVMGVIGMLFAIRDWWPLFVAGCWLYMALSPAVEASEQTVIQK
                     VVPYQRQGRVFGFASAMEAAAAPVTAFLIAPIAQYVIIPFIESADGRGAWGWLLGEGQ
                     ARGIALIFVFAGIAIVLLVLIAFGTRAYRRLSDSYADAAVPQIAAGSA"
     gene            5233..5880
                     /locus_tag="VN041_01530"
     CDS             5233..5880
                     /locus_tag="VN041_01530"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWU27760.1"
                     /translation="MTDTHETSERPLLSADRLKAFTDGVVAIAMTLLILPLMEGVIDL
                     GREGRTVLEYLSEDFGQLFSFVMSFVLIASLWISHHRIFDRVERTSGALIGLTIAWMF
                     TIVWLPVPTAMLGAMPDDVAQKCLYVGTLIVANLILLAIRAYLRRHRELHDIPDGLLR
                     DGIRADAVTVALFVVALIVALLVPQIGYFAMFLMILTGPAIRLVKRLERGRRRAR"
     gene            complement(5843..6487)
                     /locus_tag="VN041_01535"
     CDS             complement(5843..6487)
                     /locus_tag="VN041_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU27761.1"
                     /translation="MNRTILLAGATSAAGLTVTRALIEAGATVIATGRSAERLAPLGD
                     AGARIEVADATSLTGMTALAERVGAVDGVVPLVGGWRGGGGLAGQSDEDFDALLPALQ
                     AVRATSRAFDASIRGSDAGRFAIVSSTVVNKPLAGGANYATVKAAAETWTRAVAQGYA
                     KAARDAGTDLHAASVIFRAEGAFAPESLARAVIALWDADAADLNGRVVDLAPAA"
     gene            complement(6588..8018)
                     /locus_tag="VN041_01540"
     CDS             complement(6588..8018)
                     /locus_tag="VN041_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HWU27762.1"
                     /translation="MSTITSTINTIIGEPEVVEDASTAETSAAWKLLKDAAIALRPLQ
                     IKDGSVPESADREAAAQHVAEIIAGIRALAPAFPHDADYLDALVVDFQRWADGGFAEP
                     DFLDSLMAFQPQQHRIDGIRHLVVFPMYTQNGSSDRLVEALIVETIWPEFIAELEAGE
                     YGNKLFVSLRLVDFTPGYDTNSAVLFPETVAMREIPSFTWGAIFQDREAARYRRVVRA
                     ASEITNLELPERAAAMLDDQQMTEKTFVMWDIIHDRTHMRGDLPFDPFMIKQRMPFFL
                     YSLEEMRCDMTAFRESVKIERAFDARVAAGEQLTETEQEMHDYAHLVQYAVIFDRIFR
                     FAITGNRTRNYDAVGGQLLFAWLHQHGVLHWTDTALAFDWDNVPDAVVALGDAIDDLY
                     WHSIDRPKIAHWLAAYELVRGTLTPHPASQWARGLSDEILAGAPKGYTDAVMDDEFPL
                     SMFFETLDKKMKPVIESTVGIRGTDD"
     gene            8091..8546
                     /locus_tag="VN041_01545"
     CDS             8091..8546
                     /locus_tag="VN041_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWU27763.1"
                     /translation="MDDAVDHALLAEISRDGRVTLAQLSETVGLSTSAVQSRLKRLET
                     RGVITGYRALLDPEQVGKPLSAFIEITPIDPGQPDNAPELLEHLTEIEACHSIAGEAS
                     YMLFVRVKGPRALEKLVNDIRSIANVRTRATIVLQTYYEHRPIVSPPAS"
BASE COUNT         1274 a         3014 c         3030 g         1427 t
ORIGIN      
        1 cggagcggct gggtggggcg ggcctgtcag gggcgcgccc gcgcggagcg gctggtagaa
       61 atgtctcatg gcggtgcgca caccagatcc tgaccccgac ggcggcttcg ggttcgacga
      121 cgtgcccgcc gcggactcca acccggcgtg gctgtccgac atcgagctcg aagaggcgcg
      181 gcgccgcctg ccgatgctgt acgtcgaggc ggtccctgtc gtcaccgacg gcgcgggcca
      241 ggtcacctcg atcggcgtgc tgctgcggtc cacgccgctc ggcgagatca cccgcaccct
      301 ggtgtccggc cgcgtccggt atggcgagac agtccgggac gccctgttcc ggcacatcga
      361 gaacgacctg ggcccgatgg ccttcccgct gctccccgtc tctccgacgc cgttcgcggt
      421 ggcggagtac ttcccgatcc ccggtgtgag cgccttccat gacgaccggc agcacgccgt
      481 gtccctggca ttcgtcgtcc ccgtgacggg gacgtgcgag ccgcgtcagg atgccctcga
      541 ggtcacctgg ttctcgccgg aggaagcagc atccgacggc ctctccgctg agatggaggg
      601 cggccgcggc acgctgctcc gccaggccct cgcgcacctc ggtctgctgc gctgacaccg
      661 gatcccacgg catccggcac ccgccgcgta ggctcggtcg ggtgagtgaa cccatcgcct
      721 tccccaccgc atccgccgac tggctcgcgt tcgcgagcac ccgcaccgac gagcgcctcg
      781 cgcgcgtcga cgagctcgcc gcccgcctga aggacggctc ctcacgcacc gcgctcgagg
      841 cgctgcagct gtggaacgac ctgcagatcg agctgcgcgg cgcgtacaac gaggccgagg
      901 tgctcgccga ggtgcaccct gaccgggccg tgcgcgaggc cgcggaaacg cgcacctccg
      961 ccgctcaggc gaaggacgcc gagctgatga ccgacgccga cctggccgcg gtgttcggcg
     1021 cggcggatgc agaggcgctc gatgccgatg ccgcccggct gctcgggaag atccagcgcg
     1081 acttccgccg cggcggcgca gaccgggacg ccgaaacccg cgagcgcctg cgcgcgttcg
     1141 ccgcccggga gaccgagctc agcgtgacct tcgcccggaa catccgtgag gggcgccgcg
     1201 agatccgtat cgccccggac aagctcgccg gactccccga ggacttcatg gctgagcatc
     1261 cggtcggcga agacggtctg gtcgcggtgt cgacggacaa cctcgacgtg atggcggtga
     1321 tgaactacgc gcacgaccgc gagacgcgca gcgcgctgcg cgtcgcccgc tccgacctcg
     1381 cctggcccga gaacgactcc gttctcgccg agctgctcga cgtccgtcag cagcacgcgg
     1441 agctgctcgg atacgccagc tgggccgact acgagaccga ggatcgcatg gcgcgctccg
     1501 gggcccgggt cgcctcgttc ctggaggagg tcgacgaggc gtcgaaggcg gcggccgagg
     1561 ccgagtacgc gatcctgctc gagcggctgc gggaggacgt cccggatgcc gagggcgtga
     1621 ccaacgctga ccagatgtat ctgctcaccc gactgcacga ggagcgcttc gccgtcgacg
     1681 cgcagcaggt gcgccgccac ctcgacttca cccgcgtcct cggcggcctg ctcagcacca
     1741 cgggacgcct gttcggcatt gagtacgccc ccgtcgacgt tcccgcctgg cacgacgagg
     1801 tgcggtcgta cgacgtgctg cgcgcggggg agcggatcgg acgcatccac ctcgacctgc
     1861 acccgcgtga gggcaagttc aaccacgccg ccgtcttcgg cctcgctccc ggcgtgcgcg
     1921 gccgcgtgct gcccgaggcg gcgctggtct gcaatttccc gaccggtctg atggagcatc
     1981 ggcaggtgca gaccttcttc cacgagttcg ggcacctcgt gcatgacatc ctgggcggcg
     2041 accaggagtg ggcgccgttc tcgggcgtcg cgaccgagtg ggacttcgtg gaggcgccca
     2101 gccagatgct ggaggagtgg gtgtgggatg ccggcgtgct ggcctccttt gcgctcgacg
     2161 agaccggcgc gcccatcccc gccgagctgg tcgcgcggat gcgggaggcg gatgccttcg
     2221 gccgggcgct gctggtgcgc acgcagctgg gccatgcccg cgtctcgtac cacctgcaca
     2281 tggaccgccc cgcggacctc gcggcggaca ccgaccactg gtacgaggtg tcgagccccc
     2341 tccagcacct gcgggggacg cactcgtacg ccgccttcgg tcacctcacc ggctacggct
     2401 cgtgctacta cacgtaccag tggagcctcg tgatcgcgcg cgacctgctc accggcttca
     2461 cggggctgat ggacgaggca tccgccaccc gctaccgccg cgagatcctc gagccgggcg
     2521 ggcgcaggga tgccgccgat ctgatcgagg cgttcctcgg tcgccccttc acggtcgacg
     2581 cctatcggga gtggctgggc gctcccgccg cctgacgccc gcacccgcat ccaccgagac
     2641 ccccagtaac cgccgagacc cctacctgca gacgtctgca ggtaggggtc tcggcgacaa
     2701 gtaggggtct cggcgaagga gaacgggcgt caggccgtgg tgagccgccc gagctcggcg
     2761 acgaacgcgt cgacgtcgga ctcctgcgtg tcgaagctgc acatccagcg cacttcgttc
     2821 cgggaggcgt cccagtcgta gaagcggaag ctctcgcgca gcttgtcggc gacgccgtcg
     2881 ggcagcacgg cgaagacgcc gttggactgc gtgggctgcg agaaggacac gccctggatc
     2941 gagccgtcgg cgagcccggc ctcgacctca ccgcgcaggc gctgggccat ggcgttcgag
     3001 tggcgcgcgt tgcgcagcca caggtcgccc tcgagcagcg cgatcagctg ggccgacacg
     3061 aagcgcatct tcgaggagag ctgcatgttg tacttgcggc tgtagatcag gccgtccgat
     3121 gcctcggggt tcagcaccac gacggcctcg ccgagcatgg cgccgttctt cgtgccgccg
     3181 aagctcagca cgtcgacgcc ggcgtcggtc gtgaacgcgc gcagcggcac gtcgagggcc
     3241 gccgcggcgt tcgagatgcg cgcgccgtcg aggtgcagct tcataccgcg ctcgtgcgcg
     3301 tggtcggcga gggccttgat ctcgtcgacc gtgtagaggg tgccgagctc ggtcgactgc
     3361 gtgatcgaga ccaccagggg ctgggcgcgg tgctcgtcgc cccatcccca cgcctcgcgg
     3421 tcgaccagtt ccggggtgag cttgccgtcg tcggacggca cggtcagcag cttgaagccg
     3481 ccgatcttct cgggagcgcc gccctcgtcc acgttgatgt gcgcggtgga ggccgagatc
     3541 acggcgcccc agcgcggcag catcgactgc agaccggtca cgttcgcgcc ggtgccgttg
     3601 aagacgggga aggcctgcac accctcgccg aagtgccgcg cgaagacctc ctgcaggcgg
     3661 gtcgtgtaga gatcctcgcc gtacgcgatc tggtggccgt cgttggccgc ggcgatcgcc
     3721 gcgagcacct cggggtggat gccggagtag ttgtcggatg cgaagccgcg gacggcggtg
     3781 tcatgcagag tgctcaccgt acaagcctag agcgtggcat ccgtcccgat ctccagacgt
     3841 ccctgtcgga tgtcggcgct cccgcctacc ctcgtcccat gcgcacattc ctgcaggtgc
     3901 tggtgaacac catggtcgcc aatgtcacca ccagcttcct gtggttcgcg ctgacgttct
     3961 gggtctacct cgagacgagg aacgtgatgg ccaccggcat cgtcggcggg gcctacatgc
     4021 tgctcatcgc gatcttctcc atcgtgttcg gcacgatcgt cgaccgacac cggaaacgcg
     4081 ccgtgttcgt cttctccagc gcgatcacga cggtcgcgtt cgtgctcgcc tggctgatct
     4141 tcctgtggca gcccgagcgc gcgctgctgg acttcggtgg gccgtggttc tggctgttcg
     4201 cggggatcat cctgctcggc gccgtggtcg agcagatgcg cagcatcgcc ctctccacca
     4261 cggtgaccct cctcgtgccg gtcgagcggc acgcgaacgc gaacggcatg gtcggaaccg
     4321 tgcagggact ggccttcctc gtgacgagcg tgttcagtgg gctctcggtc gggtttctcg
     4381 ggatgggagg gacgctcctg atcgccctcg ggctgacggt cgtctcgctc gtgcacatcc
     4441 tgttcatccg catcccggag gagcagccgc agcacgatcc cgatgcgccg tccgccatgg
     4501 atctgcgcgg cagcatcagg gcgatcagag ccgtgcccgc gctgttcgca ctgctgttct
     4561 tctcgatgct gaacaacctg gtcggtggcg tctacatggc gctgatggac ccgtacggcc
     4621 tcgaactgat gtcggtgcag atgtggggca tcgcgctcgg ggtcgcgtcg acgggcttcc
     4681 tgatcggcgg catcgtcgtc tcgacggccg gcctcggcaa gaacccgatc cgcaccatgc
     4741 tgatgctcgt cggagtgatg ggcgtgatcg ggatgctgtt cgcgatccgc gactggtggc
     4801 cgctgttcgt ggcgggctgc tggctgtaca tggccctctc gccggctgtc gaggcgtccg
     4861 agcagaccgt catccagaag gtcgtgccgt accagcggca ggggagggtg ttcggcttcg
     4921 cctcggccat ggaggcggcc gccgcccccg tcacggcgtt cctcatcgcc ccgatcgccc
     4981 agtacgtgat catcccgttc atcgagtccg ccgacggacg gggagcctgg ggctggctgc
     5041 tcggcgaggg acaggcgcgc ggcatcgccc tgatcttcgt gttcgccggg atcgcgatcg
     5101 tcctgctcgt gctgatcgcc ttcggcaccc gcgcctaccg ccggctcagc gactcctacg
     5161 cggacgcagc ggttccgcag atcgccgcgg gcagtgcttg acggggcgac gacctcgcga
     5221 gagcattccc cggtgaccga cacgcacgag acgtcagaac gtccactgct ctccgccgac
     5281 cggctgaagg ccttcaccga cggggtggtc gccatcgcga tgaccctgct gatccttccg
     5341 ctgatggagg gcgtcatcga cctcgggcgc gaggggcgca cggtgctcga atacctcagc
     5401 gaggacttcg ggcagctgtt cagcttcgtg atgagcttcg tgctgatcgc ctcgctctgg
     5461 atctcgcacc accggatctt cgaccgggtc gagcgcacgt ccggcgcgct gatcgggctg
     5521 acgatcgcct ggatgttcac gatcgtgtgg ctgcccgtgc cgacggcgat gctgggcgcc
     5581 atgcccgacg acgtggcgca gaagtgcctg tacgtcggca ccctcatcgt cgcgaacctc
     5641 atcctgctgg cgatccgcgc ctacctgcgg cgccatcggg agctgcatga catccccgac
     5701 ggactcctgc gcgacggcat ccgcgccgat gcggtgaccg tcgctctgtt cgtcgtcgcg
     5761 ctgatcgtcg cgctgctcgt gccgcagatc ggctacttcg cgatgttcct gatgatcctg
     5821 accggcccgg cgatcaggct ggtcaagcgg ctggagcgag gtcgacgacg cgcccgttga
     5881 ggtcggcggc atccgcgtcc cacagggcga tgaccgcgcg tgcgagggat tccggggcga
     5941 acgcgccctc agcacggaag atcacggatg ccgcgtgcag gtcggttccc gcgtcgcggg
     6001 cggccttcgc atatccctgg gcgacggcgc gggtccacgt ctcggcggcg gccttgacgg
     6061 tcgcgtagtt cgccccgccc gccagcggct tgttcacgac ggtggacgac acgatcgcga
     6121 agcgaccggc atccgacccc ctgatcgagg cgtcgaaggc gcggctcgtc gcgcgcaccg
     6181 cctgcagggc gggcagcagt gcgtcgaagt cctcgtcgct ctgaccggcg agcccgccgc
     6241 ctccgcgcca tccgccgacc agcgggacga cgccgtcgac ggccccgacg cgctcggcga
     6301 gtgccgtcat gccggtcagg ctcgtggcat ccgccacctc gatccgtgcg ccggcgtctc
     6361 caagaggagc gaggcgctcg gcagaacgac cggtcgcgat caccgtcgcc cccgcctcga
     6421 tcagggctcg cgtgaccgtg aggcctgcag cactcgtcgc accggcgaga aggatcgtgc
     6481 ggttcatggt cgctccctgc gtcgcgtatg aaggatggat gcgagtctga aggacgctcc
     6541 gccggaaatc atccttcaga agcggagacg tccttcagaa ccgcgcgtca gtcgtcggtg
     6601 ccgcggatac cgacggtcga ctcgatgacc ggcttcatct tcttgtcgag ggtctcgaag
     6661 aacatcgaca gcgggaactc gtcgtccatc acggcatcgg tgtagccctt gggagctccc
     6721 gcgaggatct cgtcggacag cccgcgtgcc cactgcgatg ccgggtgcgg ggtgagcgtg
     6781 ccgcggacga gctcgtaggc cgccagccag tgcgcgatct tcgggcggtc gatcgagtgc
     6841 cagtacaggt cgtcgatcgc gtcacccagc gcgacgacgg cgtccggcac gttgtcccag
     6901 tcgaaggcga gcgcggtgtc ggtccagtgc agcacgccgt gctggtgcag ccaggcgaac
     6961 agcagctgac cgccgaccgc gtcgtagttg cgggtgcgat tgcccgtgat cgcgaagcgg
     7021 aagatgcggt cgaagatcac cgcgtactgc acgaggtgcg cgtagtcgtg catctcctgc
     7081 tcggtctcgg tgagctgctc gcccgcggcg acgcgggcgt cgaacgcgcg ctcgatcttc
     7141 acggactcgc ggaacgccgt catgtcgcag cgcatctcct cgagggagta caggaagaac
     7201 ggcatccgct gcttgatcat gaacggatcg aagggcagat caccgcgcat gtgcgtgcgg
     7261 tcgtggatga tgtcccacat cacgaacgtc ttctcggtca tctgctggtc gtcgagcatg
     7321 gccgcggcgc gctcgggcag ttcgaggttg gtgatctcgg atgctgcgcg gaccacgcgg
     7381 cggtagcggg ccgcctcgcg gtcctggaag atcgcgcccc aggtgaagga ggggatctcg
     7441 cgcatcgcga cggtctcggg gaacagcacg gccgagttcg tgtcgtagcc gggggtgaag
     7501 tcgaccagac gcagcgagac gaacagcttg ttgccgtact cgccggcctc gagctcggcg
     7561 atgaactccg gccagatcgt ctcgacgatc agggcctcca ccagacggtc gctcgagccg
     7621 ttctgggtgt acatcgggaa gacgacgagg tgacggatgc cgtcgatgcg gtgctgctgg
     7681 ggctggaacg ccatcagcga gtcgaggaag tcgggctcgg cgaagccgcc gtccgcccag
     7741 cgctggaaat cgaccacgag tgcgtcgagg tagtcggcgt cgtgcgggaa tgcgggggcg
     7801 agcgcgcgga tgcccgcgat gatctcggcg acgtgctggg cggcggcctc acggtcggcg
     7861 gactccggca cggagccgtc cttgatctgc agcgggcgca gcgcgatcgc ggcatccttc
     7921 agcagcttcc aggccgcgct ggtctcggcg gtcgacgcgt cctccacgac ctcgggctcg
     7981 ccgatgatgg tgttgatggt gctggtgatg gtggacatcg aaccctccga tcgtggtcag
     8041 aaacggaaat tttccggcga gagccggttt cagcaactat tattccactc atggatgatg
     8101 ctgtcgacca cgcgctcctc gccgagatct cccgggacgg gcgggtgacg ctcgcccagc
     8161 tctccgagac cgtcggactg tccacctccg ccgtgcagtc ccggctcaag cgcctggaga
     8221 cccgcggagt catcacgggc tatcgggcgc tgctcgaccc cgagcaggtc ggcaagccgc
     8281 tgtcggcgtt catcgagatc acgccgatcg atcccggcca gcccgacaac gcccccgagc
     8341 tgctggagca cctgaccgag atcgaggcat gccattccat cgccggcgag gcgagctaca
     8401 tgctgttcgt ccgggtgaag ggcccccggg ctctcgagaa gctggtcaac gacatccgct
     8461 cgatcgccaa tgtgcgcacg cgcgcgacga tcgtgctgca gacctactac gagcaccgcc
     8521 cgatcgtctc gccgccggcg tcctgaccgg cggagggcgc ccgctcgata ggattcggtc
     8581 gtggcaggta cctccaagaa gtcgcgcacg tcctcgcgca cgcacgcgcc gcagaagcgg
     8641 cagacctccg gcaacccggc gaagcggccc gtgatcgagg cgggcccggt gaccttcggc
     8701 gactgggtgg gcgcggctcg gctgcgcacc ctgcccctgg ctgtc
//
LOCUS       DATMSX010000051         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_741, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000051 DATMSX010000000
VERSION     DATMSX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VN041_01550"
     CDS             complement(<1..968)
                     /locus_tag="VN041_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27764.1"
                     /translation="MPQGQVLEFNRVTKRFGDVTAVADLSVRVEPGVVTAFLGPNGAG
                     KTTSLRMLLGQIRPTSGTATIGGSAYSELRQPLRSIGAVLEEASYRPRRTAARQLSIA
                     AKANGIPASRVDEVLRLVGLQDDADTRLGSYSLGMRQRLAVASALLGDPGVLVLDEPA
                     NGLDPEGIRWMRLLMRRLADEGRTVLVSSHVLSEIEQVADDVVVLSKGRAVFAGSIDK
                     LADPAGGAVIVDAEHRAQLITALSAAKLNFDVLRSGVTVRDSDAATVGAITAAAGVPL
                     TLLQQRGPSLEDVFLDLVYGRRSDSPRLDQVANPVAHAPQDEAASTP"
     gene            1103..>1870
                     /locus_tag="VN041_01555"
     CDS             1103..>1870
                     /locus_tag="VN041_01555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWU27765.1"
                     /translation="MTDAEAATVLVRTENSLGRITLNRPRAINALDQDMIARITEALK
                     AWARDPDIETVLIDGEGERGLCAGGDVRALYEQLVGGHPGDVAAFFRAEYAMNAMIAE
                     YPKPVVAFADGITMGGGIGLAGHAAVRIVTERSKLAMPETRIGFTPDVGGTWLLGRAP
                     GRLGEYFGLTGQSMTGADAVQLGFADHYVPSERLDALREALAWRADPGTPNELVLLFD
                     ETPDPSPALAARAWIDEVFSADTVGEIVSRLRASEEPD"
BASE COUNT          283 a          648 c          679 g          260 t
ORIGIN      
        1 ggcgtggaag ccgcctcgtc ctgcggtgcg tgcgcgacgg ggttggccac ctggtccagg
       61 cgcgggctgt ccgagcggcg accgtagacg aggtcgagga agacgtcctc caagctgggg
      121 ccgcgctgct gcagcagggt caggggcacg ccggcagcgg cggtgatggc gccgaccgtc
      181 gcggcatccg agtcccgcac ggtcacgccc gagcgcagca cgtcgaagtt cagcttggcc
      241 gccgagagcg cggtgatcag ctgcgcgcgg tgctcggcat ccacgatgac ggcgccgccg
      301 gccggatcgg cgagcttgtc gatcgagccg gcgaacacgg cgcgaccctt cgacagcacc
      361 accacatcgt cggcgacctg ctcgatctcg ctgagcacgt gcgaggacac cagcacggtg
      421 cggccctcgt cggcgaggcg acgcatcagc aggcgcatcc accggatgcc ctcggggtcg
      481 agtccgttcg ccggctcgtc gagcacgagc acgcccgggt cgccgagcag agcggatgcc
      541 acggccaggc gctggcgcat gccgagcgag tagctgccca gacgcgtgtc ggcgtcgtcc
      601 tgcaggccga ccagacgcag cacctcgtcg acgcgggagg ccgggatgcc gttcgccttg
      661 gcggcgatcg acagctggcg cgcggcggtg cggcgcggac ggtacgaggc ctcttcgagg
      721 acggcaccga tcgagcgcag cggctggcgc agctccgagt aggcggagcc gccgatcgtc
      781 gccgtgcccg aggtcgggcg gatctggccg agcagcatcc gcagcgacgt cgtcttgccg
      841 gccccgttcg ggccgaggaa agccgtgacg acgccgggtt cgacgcgcac ggacaggtcg
      901 gcgaccgcgg tcacgtcgcc gaagcgcttc gtgacccggt tgaactcgag cacctgtccc
      961 tgaggcatga cagtctcccc ctgttgcagt cagttcctcc tatcttctcg gaaaaccctg
     1021 tgaatccggg tctcaccttg ccggaaaccg cgatcccggc ccccgaatag gggcgaaacg
     1081 ccaagcggag tagcctgacg gcgtgaccga tgccgaagcc gccactgtcc tcgtccgcac
     1141 tgagaactcc ctcgggcgca tcacactgaa ccgtccgcgg gccatcaatg cgctcgacca
     1201 ggacatgatc gcgcgcatca ccgaggcgct gaaggcgtgg gcgcgcgacc ccgacatcga
     1261 gaccgtgctg atcgacggcg agggcgagcg cggactgtgc gcgggcggtg acgtgcgcgc
     1321 gctgtacgag cagctcgtcg gagggcatcc gggtgatgtc gccgcattct tccgtgcgga
     1381 gtacgccatg aacgcgatga tcgcggagta cccgaagccg gtcgtcgcct tcgcggacgg
     1441 catcacgatg ggcggcggca tcggcctggc cggtcatgcg gcggtgcgga tcgtcacgga
     1501 gcgctccaag ctcgcgatgc ccgagacgcg catcggcttc acccccgacg tcggcggcac
     1561 ctggctgctc ggacgcgcgc cgggcaggct gggggagtac ttcggcctga ccgggcagag
     1621 catgaccgga gcggatgccg tgcagctcgg cttcgccgac cactacgtcc cctctgagcg
     1681 gcttgacgcg ctgcgcgagg cgctggcgtg gcgggccgat cccggcaccc cgaacgagct
     1741 ggtgctgctg ttcgacgaga cccccgaccc gtcgccggcg ctcgccgcac gcgcctggat
     1801 cgacgaggtg ttctcggccg acaccgtcgg cgagatcgtc tcgcggctgc gggcatccga
     1861 ggagccggat
//
LOCUS       DATMSX010000052         7770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_751, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000052 DATMSX010000000
VERSION     DATMSX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1365)
                     /locus_tag="VN041_01560"
     CDS             complement(46..1365)
                     /locus_tag="VN041_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU27766.1"
                     /translation="MMNRRGMWAAAVTATAALALSGCAFGGGAPAGAGSDLKKDGAAT
                     GEITIWSWDVAATALKRLGADYEKTHKGTKITVVDIGYDNAYDKISVGLQAGTGLPDV
                     ITLETDKNQSYITQFPKGFTDLTPVFGDEKADFDPFKWDTGTDKDGKLRMAPWDSGTV
                     GLFYRTDYFEAAGIDPASMKTWDDLVAAGETIKAKTGHTLLTADLSSGGPFSMMLQQQ
                     GQGLFDENGDIAVSTPAAVQTLTLLQTMQQKGLIKNAKGWDASVTSAKDGDSAVTPEA
                     VWWIGTLEGEAPELSGKYAVRDLPVFAGGGAPTSNNGGSGMAIPAQAKNPQLAADFMA
                     FVLANTDNQASMMKDEGLFPAYLPALKSDYFQQSSDYFSGQKVFQTFAELTPKIPSIT
                     FTSDQSVAADAVSNAVAAAVLNGEDPKKALDDAAKQIANSTGRKIAG"
     gene            complement(1555..3225)
                     /locus_tag="VN041_01565"
     CDS             complement(1555..3225)
                     /locus_tag="VN041_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27767.1"
                     /translation="MAVAVTDVAELAVLAGRHLDLVTAPFTLPRSRVLVFRAEDGDGV
                     RVHTSEYEKRLSACRVLDAVVVTDAAGQALPVTDVLPHRIAFGRQAGDASATLTFDGP
                     GALSIAGVDGVRVMLTLPDGREEPHPLSGAGIRIRVAPDRSVTVEAGDHPAALAACER
                     EWLDWFANCPRVRDDLQQMAAFCWWVLGANIVELPSLGDARAVVPSKIGYVGLWQWDA
                     YFIAVGLRHGDPELAREQLEIAFRFPSPDGQLPDVVHEQGILATSDDLPESDREGLRR
                     AGSQIADPSKPVPLTKPPLAAWALRKVLEVEDSPEWAREQLQRVIRSQDWWFAGSDLD
                     GDGMPEYGHPYSSGLDDSPIFDGPIPTAAPDLGAYLVCQDREIAGLLGRYEPGAETTR
                     FLERAERTQGLLDEMWDAAAGRFLARGAGEQIRSDTVVGLMPLRTGTLQAGIRDALRA
                     AVDDPARYALEWGLPTVAASDPDFSPERMWRGPIWINTSALIAEGLEASGYPERAREL
                     REQTVRLVVHGGGPHEYYNPRTGMKAERATTAFGWSAALFIDLAVALS"
     gene            complement(3225..4166)
                     /locus_tag="VN041_01570"
     CDS             complement(3225..4166)
                     /locus_tag="VN041_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU27768.1"
                     /translation="MSATATPRLTDGGRAPATDSTIAIVAPGSKPRRPGGAGRRRAGA
                     VALHVLLILAGISMVFPFIWMLLTSLKTLPQLLQNPLEFLPNPWTVKNYTDAWEAVPF
                     GQAYLNSAYITLLVVVGTLITASMAGYAFARIKFKGSRVLFIVFLATQMIPAQVTLIP
                     FYLLMSNLGWVDSHLALIVPGMIANPFAVFLMRQFVLSLPKELEEAALVDGAGRLRIF
                     FSVVLPNLRPGLAALAIITSLGVWNAFLFPLVLLNSPDLFTVPLLLTSFESQHGSINY
                     GMVMAASAIATVPMLIVFVIGQRKILNSMAASGLGGR"
     gene            complement(4163..5128)
                     /locus_tag="VN041_01575"
     CDS             complement(4163..5128)
                     /locus_tag="VN041_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU27769.1"
                     /translation="MTQLTEAERMPQATPAASALPPLRKEAAWRRRDRMWGYVFVGPQ
                     LIGMGLFVVLPFAASLVLAFASWDGLGELSWVGFDNFVDQLQDPLLGRSILNTLLIAL
                     ITVPIGLGLAVVVAVALERLKTRALYLVLFFSPVVTATVAIAMIWQQMFRVDGVLSTT
                     IAKIFHIDPPNWLQDPRLALLAVCIVTIWSSLGLNVVIFLAGLQNISPSVIEAARIDG
                     AGAVRLLVSIRLPLLSPIVFFSSVIAFISSLQTFDIVYVLVSGGGPDNATRTIVYHIY
                     DLGFGRFEFGPSSAASIILLVLTLIITAIQFAAQKKFVHYEDDPA"
     gene            complement(5130..6446)
                     /locus_tag="VN041_01580"
     CDS             complement(5130..6446)
                     /locus_tag="VN041_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU27770.1"
                     /translation="MSRSTKRKYLAGIAVAALTLGLAACSGGGGGGGGADSAGNSTVV
                     WSTWGSADELTRYKEFNADFMKRHPDIKVKFQPVAGYGDYHSKLLAQLTSNTAPDVFY
                     VGDDMIGQFVASKRLMPLKSLMESKDSKTKVDDFFPGLFGAAENDGEIYAAPNDSNPD
                     VLWYDKEALKTAGITEDPATLAENGEWTTDKYLEMNAKLHDKKLTGSMFWNYWATHWS
                     WLSSQGVASPYSDSGEFVGNKDADTVAAMKQLGGLFQDGTFVVADTLPDGAGADSVFV
                     THKAGFFVQGRYTIGTVKSAGVQDSYDIVRWPTPDGKAAPTGVATSFLAINGKTKVKD
                     AAFTFWTEFLSAEGQIFRLKGGGNAVPSIKGADEVVLEDNYPAHAQTFLDMRDIGFED
                     YPAEAQIPGLPVAISEEFQKLYEGKTDAQQSLDAVAKLVEDRSAKK"
     gene            6586..7521
                     /locus_tag="VN041_01585"
     CDS             6586..7521
                     /locus_tag="VN041_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HWU27771.1"
                     /translation="MPLLVTVPNERLATNIGEVPADVDLRIWDMRSPAPADHLDIVVP
                     PYMGSTGSLEALEGLSVGLVQSQSIGYDGVDEILPAGIPFANAASVHETATAELAVGL
                     MIAAQRQFPRFAAAQQRGEWAPVFADSLADRRVLLVGFGGVGKAIAQRLAPFEVSITA
                     LARSARNEHVEGLGDVAVHGIDELPALLPDAEIVVLSLPGNAETHHLFDEAMIARMAA
                     DALLVNVGRGPLVDTDALLPALQEGRIRAASDVFEQEPLPEGHPLWKAPNLIISPHNG
                     GAATAMNPRMARLVRSQIERMLAGEDPLNVVIPAR"
     gene            complement(7634..7750)
                     /gene="rrf"
                     /locus_tag="VN041_01590"
     rRNA            complement(7634..7750)
                     /gene="rrf"
                     /locus_tag="VN041_01590"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1266 a         2555 c         2713 g         1236 t
ORIGIN      
        1 cgacggcgtg cgccgcggcc ccgggtggtg ctgtcgaggg acatgtcagc cggcgatctt
       61 ccgaccggtc gagttcgcga tctgcttcgc cgcgtcgtcg agcgccttct tggggtcctc
      121 cccgttcagc acggctgccg cgaccgcgtt ggagaccgcg tcggcggcga ccgactggtc
      181 ggacgtgaac gtgatcgagg ggatcttcgg cgtcagttcg gcgaacgtct ggaacacctt
      241 ctggccgctg aagtagtcgc tggactgctg gaagtagtcg gacttcagcg ccggcaggta
      301 cgccgggaac aggccttcgt ccttcatcat gctcgcttgg ttgtcggtgt tcgcgaggac
      361 gaatgccatg aagtcggccg cgagctgcgg gttcttcgcc tgcgcgggga tcgccatccc
      421 ggatccgccg ttgttggacg tgggtgcacc gccgccggcg aagaccggca ggtcgcgcac
      481 ggcgtacttc ccggacagct cgggcgcctc gccctcgagg gtgccgatcc accagaccgc
      541 ctcgggagtg acggcggagt cgccgtcctt ggcgctggtg acactggcat cccagccctt
      601 ggcgttcttg atcagaccct tctgctgcat ggtctgcagc agcgtgaggg tctgcacggc
      661 cgcgggagtg ctgaccgcga tgtcgccgtt ctcgtcgaac agcccttgcc cctgctgctg
      721 cagcatcatc gagaacggtc cacccgagga caggtcggcg gtgaggagcg tgtgcccggt
      781 cttggccttg atcgtctcac ctgcggcgac gaggtcgtcc caggtcttca tggatgccgg
      841 gtcgatccct gcggcctcga agtagtcggt gcggtagaac aggccgaccg tgccggagtc
      901 ccacggcgcc atgcgcagct tgccgtcctt gtcggtgccg gtgtcccact tgaacgggtc
      961 gaagtcggcc ttctcgtcgc cgaacaccgg cgtgaggtcg gtgaagccct tggggaactg
     1021 cgtgatgtag ctctggttct tgtcggtctc gagcgtgatg acgtcgggca ggcccgtgcc
     1081 ggcctgcagc ccgacggaga tcttgtcgta ggcgttgtcg tagccgatgt cgacgacggt
     1141 gatcttggtg cccttgtggg tcttctcgta gtccgccccg agccgcttga gcgcggtggc
     1201 cgcgacatcc cacgaccaga tggtgatctc gccggtggcg gccccgtcct tcttgagatc
     1261 gctgccggcg ccggccggcg caccaccgcc gaaagcacag ccagaaaggg ccagcgcagc
     1321 cgtcgccgtg acggcggcag cccacatacc gcgtcgattc atcatgacct cctcgtcaca
     1381 caccagattg gatattgaat acagtacacg gatggtgacg ataacaccag gatggatgcc
     1441 cgagccgagc gagcacagaa agagggccga cgcgccagat gtcggacggt ttcgcgggaa
     1501 acgtccgtga tctggcgtgt cggcccccgg aatggagctg gaccgcaggt cgcctcagct
     1561 gagcgcgacg gcgaggtcga tgaacagcgc cgccgaccat ccgaacgccg tcgtcgcacg
     1621 ctcggccttc atgccggtgc gcgggttgta gtactcgtgc ggtccgccgc cgtgcacgac
     1681 gaggcgcacg gtctgctcac gcagctcgcg ggcccgttcc ggatacccgg acgcctcgag
     1741 gccctcggcg atcaatgcgc tggtgttgat ccagatcgga ccgcgccaca tgcgctccgg
     1801 cgagaagtcg ggatcggatg ccgccaccgt gggcagcccc cactccagtg cgtagcgcgc
     1861 gggatcgtcg acggcggcgc gcagcgcgtc gcggatgccc gcctgcagcg tcccggtgcg
     1921 cagcggcatc aggccgacga ccgtgtcgga gcggatctgc tcccccgcgc cccgggcgag
     1981 aaagcgtccg gccgcggcat cccacatctc gtccagcagc ccctgcgtgc gctcggcgcg
     2041 ctcgaggaag cgcgtggtct cggcaccggg ttcgtaccgt cccagcaggc ccgcgatctc
     2101 gcgatcctgg cacacgagat acgcgccgag atcgggagct gcggtgggga tcggcccgtc
     2161 gaagatcggg ctgtcgtcca gaccggagga gtacggatgc ccgtattcgg gcatcccgtc
     2221 gccgtccaga tcggagccgg cgaaccacca gtcctgggag cggatcaccc gctgcagctg
     2281 ctcgcgggcc cactccgggg agtcctcgac ctcgagcacc ttgcgcagcg cccaggccgc
     2341 caggggcggc ttggtcagcg ggaccggctt ggaggggtcg gcgatctgcg agcccgcccg
     2401 ccgcaggcct tcccggtcgc tctcgggaag gtcgtcactg gtggccagga tgccctgctc
     2461 gtgcaccacg tcgggcagct ggccgtcggg gctcgggaag cggaacgcga tctcgagctg
     2521 ctcgcgagcg agctccgggt cgccgtgccg gagaccgacg gcgatgaagt aggcgtccca
     2581 ctgccacaga cccacgtagc cgatcttcga cggcaccacc gcacgggcat cgcccagcga
     2641 ggggagttcg acgatgttcg cgccgagcac ccaccagcag aaggccgcca tctgctgcag
     2701 gtcgtcacgc acccgcgggc agttcgcgaa ccagtccagc cactcccgct cgcaggcggc
     2761 gagcgcggcc gggtgatcgc cggcttcgac ggtgacggag cggtcggggg ccacgcggat
     2821 gcggatgccc gcgccggaga gcggatgcgg ctcctcccgg ccatccggaa gagtcagcat
     2881 gacgcgcacg ccgtcgacgc cggcgatgct cagagcaccg gggccgtcga aggtcagcgt
     2941 ggccgacgca tcgccggcct gccggccgaa ggcgatgcgg tgcggaagca cgtcggtcac
     3001 cgggagcgcc tgccccgcgg catccgtcac gacgaccgca tccagcaccc ggcaggcgct
     3061 gagccgcttc tcgtactcag aggtgtgcac gcgcacgccg tcgccgtcct ccgcccggaa
     3121 caccagcacg cgggagcgcg gcagggtgaa cggcgcggtc accaggtcga ggtggcgtcc
     3181 ggcgaggacg gcgagctcag cgacatccgt cacagcaacc gccatcaccg acctcccagg
     3241 cccgacgcgg ccatgctgtt gaggatcttg cgctggccga tcacgaacac gatcagcatc
     3301 ggcaccgtcg cgatcgcgga tgccgccatc accatgccgt agttgatcga gccgtgctgg
     3361 ctctcgaacg aggtgagcag cagcgggacc gtgaacaggt cgggcgagtt cagcagcacg
     3421 agcgggaaca ggaaggcgtt ccagacaccc agcgaggtga tgatcgccag tgccgccagg
     3481 ccggggcgca ggttcggcag caccacgctg aagaagatgc gcagacgacc ggcgccgtcc
     3541 acgagcgcgg cctcctcgag ctccttcggc agcgacagca cgaactggcg catgaggaac
     3601 accgcgaacg ggttcgcgat catcccgggc acgatcagcg ccaggtgcga gtcgacccag
     3661 cccagattcg acatgagcag gtagaacggg atcagggtca cctgggcggg gatcatctgc
     3721 gtcgcgagga acacgatgaa cagcaccctg ctgcccttga acttgatgcg cgcgaacgcg
     3781 tagcccgcca tcgacgccgt gatgagcgtg ccgaccacga cgagcagcgt gatgtacgca
     3841 ctgttcagat acgcctgccc gaagggcacg gcctcccagg catccgtgta gttcttgacc
     3901 gtccaggggt tgggcaggaa ctcgagcgga ttctgcagca gctgcggcag cgtcttcagc
     3961 gaggtcagca gcatccagat gaacgggaac accatcgaga tgcccgcgag gatcagcagc
     4021 acgtgcagcg cgacggcccc ggcgcggcgg cggccggcgc cgccagggcg acgcggcttc
     4081 gatccgggcg cgacgatggc gatcgtcgag tcggtcgcgg gtgcgcggcc tccgtcggtc
     4141 aggcggggag tggcggtggc gctcatgcgg gatcgtcctc gtagtgcacg aacttcttct
     4201 gggcggcgaa ctggatcgcc gtgatgatga gcgtcaggac cagcaggatg atgctcgccg
     4261 cgctcgacgg gccgaactcg aagcgcccga agccgaggtc gtagatgtga tagacgatcg
     4321 tgcgggtggc gttgtcgggt ccgccgccgg agaccagcac gtagacgatg tcgaaggtct
     4381 gcagcgacga gatgaacgcg atcaccgacg agaagaacac gatcggggag agcaggggca
     4441 ggcggatgct gaccagcagg cgcactgcgc cggcgccgtc gatgcgcgcc gcctcgatca
     4501 ccgagggcga gatgttctgc agaccggcca ggaagatcac gacgttcagg ccgagcgacg
     4561 accagatggt cacgatgcag acggccagca gggcgagccg cgggtcctgc agccagttcg
     4621 gcggatcgat gtggaagatc ttcgcgatgg tggtggagag caccccgtcc acgcggaaca
     4681 tctgctgcca gatcatcgcg atggcgacgg tggcggtcac caccggcgag aagaacagca
     4741 ccaggtagag ggcacgggtc ttcagccgct ccagggcgac ggcgacgacc accgccaggc
     4801 cgaggccgat cggcacggtg atcagggcga tcagcagggt gttcagaatc gagcgcccca
     4861 gcagcgggtc ctgcagctgg tcgacgaagt tgtcgaagcc gacccagctc agctcgccga
     4921 ggccgtccca gctcgcgaac gccagcacga ggctcgcggc gaacggcagc acgacgaaca
     4981 ggcccatgcc gatgagctgc ggcccgacga agacgtagcc ccacatccgg tcgcggcggc
     5041 gccaggccgc ctccttgcgc aatggcggaa gggcggatgc cgcaggggtg gcctgcggca
     5101 tccgctccgc ctcagtgagc tgagtcatgc tacttcttcg ccgaccgatc ctcgaccagc
     5161 ttcgcgacgg cgtcgaggct ctgctgcgcg tcggtcttgc cctcgtagag cttctggaac
     5221 tcctcgctga tcgcgacggg caggcccggg atctgcgcct cggcggggta gtcctcgaag
     5281 ccgatgtcgc gcatgtcaag gaaggtctgc gcgtgtgcgg gatagttgtc ctcgagcacg
     5341 acctcgtcgg cgcccttgat cgacggcacg gcgtttccgc cgcccttcag gcggaagatc
     5401 tggccctcgg ccgagaggaa ctccgtccag aaggtgaagg ccgcatcctt gaccttggtc
     5461 ttgccgttga tggcgaggaa gctggtggcg acaccggtgg gcgcggcctt gccgtcgggc
     5521 gtgggccagc ggacgatgtc gtaggagtcc tgcacgccgg cggacttcac ggtgccgatc
     5581 gtatagcgcc cctgcacgaa gaagcccgcc ttgtgcgtga cgaagacgct gtcggcgccc
     5641 gcgccgtcgg gcagggtgtc ggcgaccacg aaggtgccgt cctggaacag gccgcccagc
     5701 tgcttcatgg cggcgacggt gtcggcgtcc ttgttgccga cgaactcacc gctgtcgctg
     5761 tacggcgagg cgacgccctg cgacgagagc cagctccagt gcgtggccca gtagttccag
     5821 aacatcgagc cggtcagctt cttgtcgtgc agcttcgcgt tcatctcgag gtacttgtcg
     5881 gtggtccact cgccgttctc cgcgagcgtg gccgggtcct cggtgatccc ggcggtcttc
     5941 agcgcctcct tgtcgtacca gagcacgtcg gggttggagt cgttgggggc ggcgtagatc
     6001 tcgccgtcgt tctcagcggc gccgaacagg ccggggaaga agtcgtcgac cttggtcttg
     6061 ctgtccttgg actccatcag cgacttcagc ggcatcaggc gcttggatgc cacgaactgg
     6121 ccgatcatgt cgtcgccgac gtagaagacg tcgggggccg tgttgctggt cagctgcgcg
     6181 agcagcttgg agtggtagtc gccgtagccg gcgacgggct ggaacttgac cttgatgtcg
     6241 gggtgccgct tcatgaagtc ggcgttgaac tccttgtagc gggtgagctc gtcggcgctg
     6301 ccccaggtcg accagacgac cgtggagttg cccgcggagt cggcgccgcc gcctccgccg
     6361 cctccgccgc tgcacgcggc gaggcccaga gtcagcgcgg caaccgcaat gccggcgagg
     6421 tacttccgtt tcgtcgagcg ggacatgccg ccacctcttt catccttgga agagccggga
     6481 ttccggcaga ttcgatcgat tagtattctg tatacagaac acagaaaagt caaggggcgt
     6541 gaccataacg agatcctcct gcggcgccgg ggctatcgtc gaaccatgcc gcttctcgtc
     6601 accgtgccca acgaacgtct tgccacgaac atcggggagg tgccggccga tgtcgacctt
     6661 cggatctggg acatgcggtc tccggccccg gcggatcatc tcgacatcgt cgtcccaccg
     6721 tacatgggct cgaccggctc gctggaagcg ctcgagggcc tcagcgtggg gctcgtgcag
     6781 agccagtcga tcggctacga cggggtcgac gagatcctgc cggccggcat cccgttcgcg
     6841 aatgcggcga gtgtgcatga gacggcgacc gccgagctcg ccgtggggct gatgatcgcc
     6901 gcccagcgcc agttcccgcg cttcgcggcc gcacagcagc gcggcgagtg ggcaccggtc
     6961 ttcgccgaca gcctggccga ccggcgtgtg ctgctggtcg gcttcggcgg agtcggaaag
     7021 gccatcgccc agcgcctggc gcccttcgag gtgagcatca cagccctggc gcgcagcgcc
     7081 cggaacgagc acgtcgaggg gctcggcgac gtcgccgtgc acggcatcga tgagctgcct
     7141 gcgctgctac cggatgctga gatcgtcgtt ctgagcctgc cggggaatgc ggagacgcac
     7201 cacctgttcg acgaggccat gatcgcgcgg atggccgccg acgccctgct ggtgaacgtc
     7261 gggcgcggac cgctggtcga cacggatgcc ctgctccccg ccctgcagga gggaaggatc
     7321 cgtgcagcgt cggacgtctt cgagcaggaa ccgctccctg aggggcatcc gctctggaag
     7381 gcgccgaacc tgatcatctc gccgcacaac ggaggtgcgg ccaccgcgat gaacccgcgg
     7441 atggcgcgac tcgtgcgtag ccagatcgag cggatgctgg cgggcgaaga cccgctgaac
     7501 gtcgtcatcc cggcgcgttg agacggcggg gagtggggct gcgaagcagt ccctctcccc
     7561 ggcggctcga cgccggcggg acgcgttaac gcaaaagagc cacccatcaa tgggtggctc
     7621 tttcacagaa gaagtccggc ggtgtcctac tctcccacag ggtcccccct gcagtaccat
     7681 cggcgctgtg aggcttagct tccgggttcg gaatgtgacc gggcgtttcc ctcacgctat
     7741 ggccgccgaa acactattga catgtatcag
//
LOCUS       DATMSX010000053         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_161653,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000053 DATMSX010000000
VERSION     DATMSX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161653"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /gene="ftsH"
                     /locus_tag="VN041_01595"
     CDS             complement(<1..1964)
                     /gene="ftsH"
                     /locus_tag="VN041_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWU27772.1"
                     /translation="MDVKKVSRNPLIYIVLIGALLFAGFLLISNLSAPTQITVTQGLD
                     LLSGKTVTKAVNTDGDQRVDLTLSKPFQGSENVQFYYVEARADQVVEAIDGADIKAAD
                     VVDVVPRATWFDGFLSLLLPLVLLGLLFWWLLSSMQGGGSKVMQFGKSKAKLVSKETP
                     TVTFADVAGADEAIEELHEIKEFLQDPAKFQAIGARIPKGVLLYGPPGTGKTLLARAV
                     AGEAGAPFYSISGSDFVEMFVGVGASRVRDLFNQAKENAPAIIFIDEIDAVGRHRGAG
                     LGGGNDEREQTLNQMLVEMDGFDPNANVIVIAATNRPDILDPALLRPGRFDRQIGVDA
                     PDLKGRQKILEVHAKGKPLSKSVELEVVARKTPGFTGADLANVLNEAALLTARSNAQL
                     IDNRALDEAIDRVIAGPQRRTRVMKDREKLITAYHEGGHALAAAAMNHTDPVTKITIL
                     PRGKALGYTMVLPLDDKYSVTRNELQDQLTYAMGGRVAEEIVFHDPTTGASNDIEKAT
                     GIARKMVTEYGMTTEVGPVKLGSEGGEMFVARDMNRGHDYSDKIAETVDEQVRALIEQ
                     AHNEAYQVLSDNRDILDRLALALLEEETLDHHRIAEIFTDVRKLPERPQWLSSTERPV
                     STLPPIAVPVREQIAADAPAPTTTTRTAGP"
     gene            complement(2080..2631)
                     /gene="hpt"
                     /locus_tag="VN041_01600"
     CDS             complement(2080..2631)
                     /gene="hpt"
                     /locus_tag="VN041_01600"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWU27773.1"
                     /translation="MRAADISEDLAQILVTEEQIHAKLDELATRVAVDYEGKDILLVG
                     VLKGAVMVMADFVRALPFHAPMDWMAVSSYGASTKSSGVVQIRKDLDTDLHGKHVLIV
                     EDIIDSGLTLSWLLENFASRGAESIEVLALLRKPEAAKVEIDCKYVGFDIPTEFVVGY
                     GLDYAERYRNLRDVAVLAPHVYS"
     gene            2721..3320
                     /locus_tag="VN041_01605"
     CDS             2721..3320
                     /locus_tag="VN041_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HWU27774.1"
                     /translation="MDLDEILKQVPIDDIAEKFGVSPDVARQAVQEGSAAIVGGLAKN
                     AATPAGSSAIEDALNRHGGFSGASKVDDVDQADGGKILGHVFGDKQQEVTESLTKSEK
                     TAGGIDFGKLLPILAPIIMGLIANAKGGSGSGNGGGGLGNILGGLLGGGQQGQASGGG
                     GLGDILGGLGGLLGGGSSSGAQGGGNVIGDILGGLFGKK"
     gene            complement(3401..>3923)
                     /locus_tag="VN041_01610"
     CDS             complement(3401..>3923)
                     /locus_tag="VN041_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate-binding domain-containing
                     protein"
                     /protein_id="HWU27775.1"
                     /translation="VREDEDGLRWIRPLLGVRRETTRAFCTASDLEFWDDPHNADERF
                     TRVRVRENVLPVLEAELGPGIAQALARTAEQLREDAEAFDEMIHETIEDIVEHAEAGI
                     SVSVAALAANPAALRNRIIRLVVDSEFGVGLTRVQTLEVARLVTDWSGQGPIDLPGCT
                     AKRAGGRIVFAAR"
BASE COUNT          582 a         1268 c         1407 g          666 t
ORIGIN      
        1 ttcggacccg ccgtgcgcgt cgtggtcgtc ggcgccggag cgtcggccgc gatctgctcg
       61 cgcacgggca cggcgatcgg aggaagcgtc gacaccgggc gctcggtgct ggagagccac
      121 tgcgggcgct cgggcagctt gcgcacgtcg gtgaagatct cggcgatccg gtggtggtcg
      181 agggtctcct cctcgagcag tgccagggcg aggcggtcca ggatgtcgcg gttgtcgctg
      241 agcacctggt aggcctcgtt gtgcgcctgc tcgatgagcg cgcggacctg ctcgtcgacg
      301 gtctcggcga tcttgtcgga gtagtcgtgg ccgcggttca tgtcgcgcgc cacgaacatc
      361 tcgccgccct cggagccgag cttgaccggg ccgacctcgg tggtcatccc gtactcggtg
      421 accatcttgc gggcgatgcc ggtggccttc tcgatgtcgt tgctggcgcc ggtggtcggg
      481 tcgtggaaca cgatctcctc ggcgacgcgg ccgcccatcg cgtaggtgag ctggtcctgc
      541 agctcgttgc gggtgacgga gtacttgtcg tcgagcggga gcaccatcgt gtagccgagg
      601 gccttgccgc ggggcaggat cgtgatcttg gtgaccgggt ccgtgtggtt catggccgcg
      661 gccgccaggg cgtgcccgcc ctcgtggtac gcggtgatga gcttctcgcg gtccttcatc
      721 acccgggtgc ggcgctgcgg gccggcgatc acgcggtcga tggcctcgtc gagggcgcgg
      781 ttgtcgatca gctgcgcgtt cgagcgtgcg gtcagcagcg cggcctcgtt gaggacgttc
      841 gcgagatcgg cgccggtgaa accgggcgtc ttgcgtgcga cgacctcgag ctccaccgac
      901 ttggacagcg gcttgccctt ggcgtgcacc tcgaggatct tctggcgccc cttcaggtcg
      961 ggggcgtcca cgccgatctg gcggtcgaaa cgaccggggc gcagaagtgc cgggtcgagg
     1021 atgtcgggac ggttggtggc cgcgatgacg atcacgttcg cgttcgggtc gaagccgtcc
     1081 atctcgacga gcatctggtt cagcgtctgc tcgcgctcgt cgttaccacc gccgaggccg
     1141 gcaccgcggt gacggccgac ggcgtcgatc tcatcgatga agatgatcgc cggggcgttc
     1201 tccttggcct ggttgaacag gtcgcgcacg cgggatgcac ccacaccgac gaacatctcg
     1261 acgaagtcgg aacccgagat cgagtagaac ggtgcgcccg cctcaccggc gacggcgcgg
     1321 gcgagcaggg tctttccggt tccgggaggg ccgtacagca gcacaccctt cgggatgcgg
     1381 gcgccgatcg cctggaactt ggcggggtcc tgcaggaact ccttgatctc gtggagctcc
     1441 tcgatggcct cgtcggcgcc ggcgacgtcg gcgaaggtga ccgtcggcgt ctccttgctg
     1501 acgagcttgg ccttggactt gccgaactgc atgaccttgc tgccaccgcc ctgcatggag
     1561 gacagcagcc accagaacag caggcccagc agcacgaggg gaagcagaag cgagaggaag
     1621 ccgtcgaacc aggtggcgcg cgggacgacg tcgaccacgt cggcggcctt gatgtcggcg
     1681 ccgtcgatgg cctcgaccac ctggtcggcg cgggcctcca cgtagtagaa ctgcacgttc
     1741 tcggagccct ggaagggctt ggacagcgtg aggtccaccc gctggtcgcc gtcggtgttg
     1801 accgccttcg tgaccgtctt gccgctgagc agatcgaggc cctgcgtgac cgtgatctgc
     1861 gtgggggccg acaggttcga gatcagcagg aaaccggcga agagcagcgc gccgatcagc
     1921 acgatgtaga tcagcgggtt gcgggagacc ttcttgacat ccatgatccg ttcagcgtac
     1981 cgcccggggc ttagcgcact gctgtgcgtt caccatcggc gtaccgtgaa tcggccatgc
     2041 gacgtctgga ggcgccggga tgcggagtcc gccggcgcgt cagctgtaga cgtgcggcgc
     2101 cagcacggcc acgtcgcgca ggttgcggta gcgctccgcg tagtcgagcc cgtagcccac
     2161 gacgaactcg gtggggatgt cgaagccgac gtacttgcag tcgatctcca ccttggccgc
     2221 ctccggcttg cgcagcagtg cgagcacctc gatggactcc gccccacggg acgcgaagtt
     2281 ctccagcagc cagctcaggg tcaggccgga gtcgatgatg tcctcgacga tcagcacgtg
     2341 cttgccatgc aggtcggtgt ccaggtcctt gcggatctgc acgacgccgc tggacttggt
     2401 gctcgcgccg tagctcgaca cggccatcca gtccatcggg gcgtggaagg gcagcgcgcg
     2461 gacgaagtcg gccatcacca tcacggcgcc cttgagcacg cctacgagca ggatgtcctt
     2521 gccctcgtaa tcgacggcca cacgggtggc gagctcgtcg agcttcgcgt ggatctgctc
     2581 ctcggtgacg aggatctggg cgaggtcttc agagatgtcc gctgcgcgca tggatcgatt
     2641 ttaggtcgtg cgtcctgtgg accaggcatc cgcactactg tgagcggtgg agcgggcatg
     2701 cgcccgcggg gaaggaagac atggacctcg acgagatcct caagcaggtg ccgatcgacg
     2761 acatcgcgga gaagttcggg gtgagcccgg atgtcgcccg tcaggccgtt caggagggca
     2821 gcgccgccat cgtcggcgga ctggccaaga acgccgcgac gccggcgggc tcctccgcca
     2881 tcgaggacgc gctgaaccgc cacggcggct tctcgggtgc ctcgaaggtc gacgacgtgg
     2941 atcaggccga cggcggcaag atcctcggtc acgtcttcgg cgacaagcag caggaggtca
     3001 ccgagagcct gacgaagtcg gagaagaccg cgggcggcat cgacttcggc aagctcctgc
     3061 cgatcctcgc tccgatcatc atgggcctga tcgcgaacgc caagggcggc tccggcagcg
     3121 gcaatggcgg cggcggactc ggcaacatcc tgggcggcct gctcgggggc ggccagcagg
     3181 gtcaggcatc cggcggtggc ggactgggcg acatcctcgg cggcctcggc ggtctgctcg
     3241 gcggcggctc ctccagcggc gcacagggcg gcggcaacgt gatcggcgac atcctcggcg
     3301 gcctgttcgg caagaagtag gctgcgcttc gacgagctca gcggactttc ttcactgggt
     3361 ccctgagctc gtcgaagggt cggtctcgga tgccgaagcc tcagcgcgcc gcgaagacga
     3421 tccgtcctcc ggcgcgcttc gctgtgcatc cgggcaggtc gatcggtccc tggcccgacc
     3481 agtcggtcac gagccgggcg acctcgagcg tctgaacccg ggtcagcccg acaccgaact
     3541 cgctgtcgac cacgagccgg atgatgcggt tgcgcagcgc cgcggggttc gcggcgaggg
     3601 ccgcgacgct caccgagata cccgcctcgg cgtgctcgac gatgtcctcg atggtctcgt
     3661 ggatcatctc gtcgaaggcc tcggcatcct ctcggagctg ctcggccgtc cgggcgaggg
     3721 cctgcgcgat gccggggccg agttcggcct cgagcaccgg cagcacgttc tcgcggacgc
     3781 gcacccgcgt gaatcgttcg tcggcgttgt gcgggtcgtc ccagaattcg agatcggatg
     3841 ctgtgcagaa cgccctggtc gtctcgcggc gcacccccag cagcgggcgg atccagcgca
     3901 ggccgtcctc gtcctcgcgg acc
//
LOCUS       DATMSX010000054         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180805,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000054 DATMSX010000000
VERSION     DATMSX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180805"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VN041_01615"
     CDS             <1..227
                     /locus_tag="VN041_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWU27776.1"
                     /translation="EPSASLVFLLPPSWDELVNRLVGRGTEDAEERARRLRTARTELA
                     AQNEFDYLVVNADVATAAREVVELSTGSSR"
     gene            305..559
                     /gene="rpoZ"
                     /locus_tag="VN041_01620"
     CDS             305..559
                     /gene="rpoZ"
                     /locus_tag="VN041_01620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWU27777.1"
                     /translation="MAGTNQGIIDPPIDNLLERVESKYELVIYASKRARQINDYYSDL
                     HEGNLFDNVGPLVDSSVEDKPLTIALHEINEDKLRIRHAE"
     gene            686..1882
                     /gene="metK"
                     /locus_tag="VN041_01625"
     CDS             686..1882
                     /gene="metK"
                     /locus_tag="VN041_01625"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWU27778.1"
                     /translation="MSALRLFTSESVTEGHPDKICDQISDSVLDGLLSVDRGSRVAVE
                     TLVTTGLVHVAGEIRTDGYVDIPTIVRDVVNGIGYTSSDTGFDGSSCGVTVSVGEQST
                     DIANGVDNAQERRDGGSTDPRDELGAGDQGIMFGFATNETPQLMPMAAWTAHRIAERL
                     TEVRRSGELAFLRPDGKTQVTLGYDGYTPKTVDAVVLSTQHHPDITQDELKALVREKV
                     IDPVLAQTGLDLAPDLKYYINPAGPFVTGGPKGDAGLTGRKIIIDTYGGAARHGGGAF
                     SGKDPSKVDRSGAYAMRWVAKNVVAAGLAERAEVQVAYAIGVARPVGLYVETFGTGTV
                     SEDVITRAIDEVFDLRPQAIIEELDLLRPIYAKTAAYGHFGRELPEFTWERTDRADEL
                     RRAAGV"
     gene            1891..>3000
                     /locus_tag="VN041_01630"
     CDS             1891..>3000
                     /locus_tag="VN041_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWU27779.1"
                     /translation="MSTVPGQAPTAGGRAEIGQSSTAEPRRIARVLIDSPLPQLDRLF
                     DYALPEDVGEVGPGMRVKAPLRTAGRVIDGYIVELDVEPDATRTLSEIESVVSTVEVM
                     PERLYRLARKAADRAAGSASDILRLAIPKRQVRVEKSWQQPEPCAPPRDDAVDRARRV
                     TAAYDGLDAVLSGSGRAAVEAIPVLQDDLPGWALLLASAAALQLAAGRSSVLVVPDYR
                     DQELLLSALATLVDEDAVVRHDARQTNPDRYRAFLRTLGDAPCVVVGNRSAVYSPVRA
                     GLVAIWDDGDPLLAEPLAPYVHARDAALIRQEEEGSALLIVGHTRSTDAERLVQHGWL
                     QDVTAARRVIPQVRLSTPQELEQTGQRVPSSAFATA"
BASE COUNT          474 a         1101 c         1004 g          421 t
ORIGIN      
        1 ccgagccgtc ggcctcgctg gtgttcctgc tgccgccgtc ctgggacgag ctggtgaacc
       61 ggctggtcgg ccgggggacc gaggatgcgg aggagcgcgc ccgccggctg cgcaccgcgc
      121 gcaccgaact ggcggcgcaa aacgagttcg attacctcgt cgtgaacgcc gacgtcgcca
      181 ccgcggcgcg agaggtcgta gaattgtcca cgggctcttc gcgctgaacg ctcgcgcgcg
      241 cccgaaaccg acttcgcgac acgtcgtcat ccgacgacag cgtcgcccga ccaggaggtc
      301 caccatggct ggaaccaacc agggcatcat cgacccgccc atcgacaacc ttctcgagcg
      361 cgtcgagtcg aagtacgagc tcgtgatcta cgcgtccaag cgcgcgcgtc agatcaacga
      421 ctactactcc gacctgcacg aggggaacct cttcgacaac gtgggcccgc tggtggactc
      481 gtccgtcgag gacaagccgc tcacgatcgc gctgcacgag atcaacgagg acaagctccg
      541 gatccgtcac gccgagtgac actccgcacc ctgtgagccg ccgacgtcca cacccggatg
      601 tcggcggctt cctgcacact gggcctgcaa cacacgcggg tgagccgaac ccgacccgcc
      661 ccgatcttcc cgctttggag aaccgatgag cgcgctgcgc ctgttcacgt ccgagtccgt
      721 caccgagggg caccccgaca agatctgcga tcagatctcc gacagcgtgc tcgacggcct
      781 gctctccgtc gaccgcggct cccgcgtcgc cgtggagacg ctcgtgacga ccggtctcgt
      841 gcacgtcgcg ggtgagatcc gcaccgacgg gtacgtcgac atccccacca tcgtccgcga
      901 cgtggtcaac ggcatcggct acacctccag cgacaccggc ttcgacggct cgtcctgcgg
      961 cgtcaccgtt tcggtggggg agcagtcgac ggacatcgcg aacggcgtcg acaacgcgca
     1021 ggagcgccgc gacggcggct ccaccgaccc gcgcgacgag ctcggcgccg gcgaccaggg
     1081 catcatgttc ggcttcgcga cgaacgagac gccccagctc atgccgatgg cggcctggac
     1141 cgcgcatcgc atcgccgagc gtctcaccga ggtgcgccgc tccggcgagc tggcgttcct
     1201 gcgtcccgac ggcaagaccc aggtcacgct cggctacgac ggctacacgc ccaagacggt
     1261 cgacgccgtc gtgctctcca cgcagcacca cccggacatc acgcaggacg agctgaaggc
     1321 gctcgtgcgc gagaaggtca tcgaccccgt gctcgcgcag accggactcg acctcgcgcc
     1381 cgatctgaag tactacatca accctgccgg ccctttcgtc accggtgggc ccaagggcga
     1441 tgcgggtctg acgggccgca agatcatcat cgacacctac ggcggcgccg cccggcacgg
     1501 tggcggagca ttcagcggaa aggacccgtc caaggtcgac cgctccggcg cctacgcgat
     1561 gcgctgggtc gcgaagaacg tcgtcgccgc cggcctcgcc gagcgcgcgg aggttcaggt
     1621 cgcctatgcg atcggcgtgg ctcgcccggt gggcctctac gtcgagacct tcggcaccgg
     1681 cacggtgtcc gaggacgtga tcacgcgcgc gatcgacgag gtgttcgacc tgcgcccgca
     1741 ggcgatcatc gaggagctcg atctgctgcg cccgatctac gccaagaccg ccgcgtacgg
     1801 acacttcggc cgtgagctgc ccgagttcac ctgggagcgc accgaccgcg ccgacgagct
     1861 gcgccgcgcc gccggcgtct gaggggcgcc gtgtccacgg tgcccggaca agcgccgacc
     1921 gcaggagggc gagccgagat cggacagtcc tcgaccgccg aaccgcggcg gatcgcccgt
     1981 gtgctgatcg actcgccgct gcctcagctg gaccggctgt tcgactacgc cctccccgag
     2041 gacgtcggcg aggtcggtcc cggtatgcgg gtcaaggcgc cgctgcgcac cgcgggtcgt
     2101 gtgatcgacg gttacatcgt cgagctcgac gtggagccgg atgccacccg cacgctctcc
     2161 gagatcgaga gcgtcgtctc gaccgtcgag gtgatgcccg agcggctgta ccggctcgcc
     2221 cggaaggccg ctgaccgcgc agccggctct gcgtccgaca tcctgcgtct cgcgatcccc
     2281 aagcgccagg tgcgcgtcga gaagtcatgg cagcagccgg aaccctgcgc cccgccgcgc
     2341 gacgacgccg tggaccgcgc ccgtcgcgtc acggcggcat acgacgggct cgatgcggtg
     2401 ctctccggct caggacgcgc cgcggtcgag gcgatccctg tgctgcagga cgatctcccc
     2461 ggatgggcgc tgctgctggc gtcggccgcc gctctgcagc tcgccgccgg gcgctcgagc
     2521 gtgctggtcg tgccggacta ccgtgatcag gaactgctgc tctccgccct ggccacgctg
     2581 gtcgacgagg atgcagtggt gcggcacgac gcgcgccaga cgaaccccga ccgctaccgt
     2641 gcgttcctgc gcacgctcgg ggacgctccg tgcgtggtgg tgggcaaccg ctccgccgtg
     2701 tactcacccg tgcgggcagg actcgtcgcc atctgggacg acggcgaccc gctgctggcg
     2761 gagccgctcg cgccctacgt gcacgccagg gatgccgcgc tcataaggca ggaggaggag
     2821 ggaagcgccc tgctgatcgt cgggcacacc cgctccacgg acgccgagcg actcgtgcag
     2881 cacggatggc tgcaggacgt caccgccgcg cggcgcgtga tcccgcaggt gcgcctgagc
     2941 acgccgcagg agctcgaaca gaccggccag cgggtgccct ccagcgcctt cgcgaccgcg
//
LOCUS       DATMSX010000055         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19585,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000055 DATMSX010000000
VERSION     DATMSX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19585"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VN041_01635"
     CDS             complement(<1..440)
                     /locus_tag="VN041_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27780.1"
                     /translation="MTVRQHPRSLMLALGALTAFGPISLDVYLPSLPQLGAEFSASES
                     LSQLTMSACMIGLALGQLLWGPISDRYGRRMPLILAIAGFTVTSVACTFAPSIEVLIG
                     IRVVQGLCGAAGMVIARAVVHDLFSGADAVAAFSTLAAVMGVAPV"
     gene            complement(437..1768)
                     /locus_tag="VN041_01640"
     CDS             complement(437..1768)
                     /locus_tag="VN041_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27781.1"
                     /translation="MFRSFRSFNYRTWFLGAIVSNIGGWMQSTAQDWVVLTELTHNDA
                     AAMGATMALQFGPPLVLVGLTGWVADRFDRRKILLATQTALMVLAIGVGVLLLTGILT
                     LPLMLCFAAAFGVTNAFDAPARQAFVSDMVSVEDTSNAVALNSASFNMARLIGPAVGG
                     IAIVALGSGWVFIANAVTFLAMLIALLLIRRGELNPRVKSGRKGGGLAAGVRYVLSRP
                     DLKVVFVMVFLLGAFGMNFPIFASTMALEFGEGAAGYGVLSSVLAIGSLAGALLAARR
                     DRARVRVIVLSAAGFGAASLISATMPVYWMYGAVLVFVGFSTVTMLTTANGYVQTTTD
                     AALRGRVLALYMAVVMGSTPIGAPIAGWVADTFGPRMAITVGGVAGFLASAIGLAWML
                     WSGRLHRAERTRFLLTLDETRPLQVVRAMSTSFDEKSAATTPIQLPRKNAE"
     gene            complement(1774..2205)
                     /locus_tag="VN041_01645"
     CDS             complement(1774..2205)
                     /locus_tag="VN041_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU27782.1"
                     /translation="MTEDFAATASELRFALFRLTRRLRGVRAVDAMSDAQLAALGALR
                     AHGRRTLSGLAEHERVTAPTMSATVNGLEELGLVVRVPDEDDRRRVYVEITAEGEKVV
                     AATIRRRDEALADMITGVGLDADELAVLRAAAPVLRKLAES"
BASE COUNT          427 a          844 c          832 g          308 t
ORIGIN      
        1 acgggcgcca cgcccatcac cgcggcgagc gtcgagaacg cggccaccgc gtcggcgccc
       61 gagaacaggt cgtgcacgac agcccgggcg atgaccatgc cggccgcccc gcacagcccc
      121 tgcaccaccc ggatgccgat cagcacctcg atcgagggcg cgaaggtgca cgccaccgag
      181 gtcacggtga accccgcgat cgccaggatc agcggcatcc gccggccgta cctgtcgctg
      241 atcggccccc acagcagctg accgagggcg agcccgatca tgcaggcgct catcgtcagc
      301 tggctgagcg actcggatgc cgagaactcg gcgccgagct gggggagcga gggcaggtag
      361 acgtcgaggg agatcggccc gaacgcggtc agtgcgccga gcgcgagcat gagcgagcgc
      421 ggatgctgcc gcacggtcac tccgcgttct tgcgcggcag ttggatcggc gtggtcgccg
      481 cggacttctc atcgaacgac gtcgacatcg ccctgaccac ctgcagtggc cgcgtctcgt
      541 cgagagtgag caggaaccgg gtgcgctcgg cgcggtgcag ccgccccgac cagagcatcc
      601 aggccaggcc gatcgcactg gccaggaaac ctgcgacacc gcccacggtg atggccatcc
      661 gcggcccgaa cgtgtcggcg acccagccgg cgatgggcgc gccgataggc gtggagccca
      721 tcaccaccgc catgtacaac gccagcacgc ggccgcgcag cgcggcatcc gtcgtcgtct
      781 gcacgtaccc gttcgcggtg gtgagcatcg tgaccgtgga gaagcccacg aagaccagca
      841 cggcgccgta catccagtac acgggcatgg tcgccgagat cagcgaggcc gcgccgaagc
      901 ccgccgccga cagcacgatc acccgcacgc gtgcccggtc gcgtcgggcc gccagcagcg
      961 ctcccgcgag cgaaccgatg gcgagcaccg agctgaggac gccgtaacct gcggcgccct
     1021 ccccgaactc gagtgccatg gtcgatgcga agatcgggaa gttcatgccg aacgcgccga
     1081 gcaggaacac catcacgaag acgaccttca ggtcgggccg gctgagcacg taccgcaccc
     1141 cggcagccag gccgccgccc ttccgtccgc tcttcacccg cgggttgagc tcgcccctgc
     1201 ggatcaggag gagcgcgatc agcatcgcca ggaacgtcac cgcgttcgcg atgaagaccc
     1261 agccggatcc gagcgccacg atcgcgattc cgcccacggc cgggccgatc aggcgggcca
     1321 tgttgaacga cgccgagttc agggcgacgg cgttcgaggt gtcctcgacg ctgaccatgt
     1381 ccgacacgaa cgcctgacgg gcgggggcgt cgaacgcgtt ggtcacgccg aaggccgcgg
     1441 cgaagcacag catcagcggc agggtcagga tgccggtcag cagcagcaca ccgaccccga
     1501 tcgccagcac catcagggcc gtctgggtgg ccagcaggat cttgcgcctg tcgaaccggt
     1561 ccgcgaccca cccggtcagt cccaccagca cgagcggcgg gccgaactgc agggccatgg
     1621 tcgcgcccat ggccgcggcg tcgttgtggg tcagctcggt gagcaccacc cagtcctggg
     1681 cggtcgactg catccagccg ccgatgttcg agacgatcgc accgaggaac caggtgcggt
     1741 agttgaacga ccggaaggag cggaacatcg agctcatgac tcggccagct tccgcagaac
     1801 gggggcggcc gcgcgcagca cggcgagctc atcggcgtcg agaccgacac cggtgatcat
     1861 gtcggccagc gcctcatcgc gacggcggat ggtcgcggcg acgaccttct cgccctctgc
     1921 ggtgatctcc acgtagacgc ggcggcggtc gtcctcgtcc ggaacccgaa cgacgaggcc
     1981 gagctcctcg aggccgttga cggtcgcgct catggtcggg gccgtcaccc gctcgtgctc
     2041 ggcgaggccg gagagggtgc gtcgtccgtg tgcccggagg gcgccgagag cggcgagctg
     2101 ggcatcgctc atcgcgtcga cggcgcggac gccgcgcagg cgtcgggtca gacggaagag
     2161 ggcgaatcgg agctcggatg cggtggcggc gaagtcctcg gtcattactt agatagccta
     2221 actaagtttg tcgcgacggg gaagcgtgaa aataatgttc accatcttgg gaatcaggcc
     2281 tccgagtgcg aaaatgcttc tcgtgccact cgcgttcgaa ctcgccgtcc agaaccccgc
     2341 cggggcgcgc atcgcccgcg aggcaggcgc cgcacgcatc gagctcgcgc aggcactcgc
     2401 gctgggcgga c
//
LOCUS       DATMSX010000056         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_806, whole
            genome shotgun sequence.
ACCESSION   DATMSX010000056 DATMSX010000000
VERSION     DATMSX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..197)
                     /locus_tag="VN041_01650"
     CDS             complement(30..197)
                     /locus_tag="VN041_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27783.1"
                     /translation="MEHTRDEPADALWSRLRLIEGQPLDARADAYGSLHDELMNRLES
                     APRGDAAPGTR"
     gene            complement(278..1315)
                     /locus_tag="VN041_01655"
     CDS             complement(278..1315)
                     /locus_tag="VN041_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HWU27784.1"
                     /translation="MALFRRAKSAPTPLTDRDALLADLDGVVYAGPGALPYAIDSLNR
                     AAEQLRLGYITNNASRTDAAVAAHLSELGLTVAPADVVTSPQAAMRLLATMVPAPATI
                     LIVGGDGLVDEAHKAGYTVTRSAEDSPAAVVQGFAPEVGWTDLAEAAFALQVPEEEGG
                     IPWISTNTDWTIPRDRGVAPGNGTLVSAVHTAIGRLSIVAGKPEKPIFEVAVERFSAQ
                     RPLFIGDRLDTDIAGAARAGIDSALVLTGIDRPKHVIAAPQGSRPDYILSDLRELHEP
                     YPETIEKNGVFTVNDAAVRVVDADVQIVAEGSSQIDLLRAGAAAIWATGLMIYAFRVP
                     ERLYADPFHKP"
     gene            complement(1315..2214)
                     /locus_tag="VN041_01660"
     CDS             complement(1315..2214)
                     /locus_tag="VN041_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27785.1"
                     /translation="MRERIEEPPIPEEITARDLHPSARNELKTLSKENADQTARHLAM
                     AAQLIDEEPERAHAHALAASRRAGRIPVVRSTLAITAYGIGDFALALRELRTYRRLSG
                     KDDMIALIVDSERGVGRPDRALEEGRAVDRAALEPEVRVALAIAMSGARLDRGETELA
                     LGELEIPELDPDRAFEWSPALFSARAAVLEDLGRTDEAAFWTERADVAASALGLDGQF
                     DEMIVEDGLIEDFDEDYDDYDDEDSEPGDGDAEDVQDAEASESDEAVESADAPEADTA
                     TIPDDASADAPEDGTDRESESEA"
     gene            2290..3138
                     /locus_tag="VN041_01665"
     CDS             2290..3138
                     /locus_tag="VN041_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27786.1"
                     /translation="MVAISEGAALALRAVTVGTTLALGPVTIRATLALGPVTTERSVS
                     LATVAVTIRATLTLRAVTIRATLTLRAVTIRATLTLRAVTIRATLALRAVTIRATLAL
                     RAVTIRATLALGTVAAVRAVALVAVAIAEGAALALGTVTVTVGTTLALRAVTVGTALT
                     LRPVTVGTALALRAVTIRTTLTLRAVPIAEGAALALRAVTIRTTLTLAAITVTMRAAL
                     ALVAIAVRSALTVTGAAGIVALGRPRGGVRVTRLALGARTSIARALTVSAVPLLIRHS
                     DSLFAC"
     gene            complement(3185..3301)
                     /gene="rrf"
                     /locus_tag="VN041_01670"
     rRNA            complement(3185..3301)
                     /gene="rrf"
                     /locus_tag="VN041_01670"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          457 a         1138 c         1203 g          523 t
ORIGIN      
        1 ccgcgccgcg agggccgcat cgagtctggt catcgcgttc ccggggccgc gtcgccgcgg
       61 ggcgcgctct cgagtcgatt catgagctcg tcgtgcaggg atccgtaggc atcggcgcgg
      121 gcgtcgaggg gctgcccctc aatcaaccgc agccgactcc acagggcgtc ggccggctcg
      181 tcgcgcgtgt gctccatccg accactgtaa cgcgcgccac cgacgagccg acggagacgc
      241 tcagggcctg tggaaaggac cagagcgcgt gaacgactca gggtttgtgg aaaggatccg
      301 cgtagagccg ctccggcact cggaacgcgt agatcatcag gcccgtcgcc cagatcgccg
      361 ccgcgccggc tcgcagcagg tcgatctgcg aggagccctc ggccacgatc tgcacgtccg
      421 cgtcgaccac acgcacggcg gcgtcgttca ccgtgaagac gccgttcttc tcgatcgtct
      481 ccggatatgg ctcgtgcagc tcgcgcaggt cgctgaggat gtagtcggga cgcgagccct
      541 gcggggcggc gatcacgtgc ttgggacggt cgatgcccgt caggacgagc gcggagtcga
      601 tgccggcacg ggcggcgccc gcgatgtcgg tgtcgagccg gtcaccgatg aacagggggc
      661 gctgtgcgct gaagcgctcg acggccacct cgaagatcgg cttctcgggc ttgcccgcga
      721 cgatcgagag ccggccgatc gcggtgtgca cggcggagac gagcgtgccg ttgcccggcg
      781 ccacgccgcg atcgcgcggt atcgtccagt cggtgttggt cgagatccac gggatcccac
      841 cctcctcctc cggcacttgc agcgcgaacg cggcttcggc gaggtcggtc cagccgacct
      901 cgggggcgaa gccctgcacg accgcggccg gcgagtcctc ggcgctgcgg gtgaccgtgt
      961 atccggcctt gtgcgcctcg tcgaccagtc cgtctcctcc gacgatgagg atcgtcgcgg
     1021 gcgcgggaac catggtcgcg agcagccgca tggccgcctg cgggcttgtc acgacgtcgg
     1081 caggagccac ggtgaggccg agctcgctga ggtgcgcggc gacagcggca tccgtgcggg
     1141 atgcgttgtt cgtgatgtat ccgaggcgca gctgttcggc ggcgcggttc aggctgtcga
     1201 tcgcgtacgg gagggcaccg ggtccggcgt agaccacccc gtcgaggtcg gcgagcagtg
     1261 cgtcacggtc ggtcagcggg gtgggcgccg acttcgcgcg gcggaacagc gccatcaggc
     1321 ctcggactcc gactcacgat cggtgccgtc ctcgggcgca tcagcggatg cgtcgtcggg
     1381 gatcgtcgcg gtatcggcct cgggggcgtc agcggactca acggcctcgt cggactcgga
     1441 agcctcggcg tcctggacgt cctcagcgtc accgtcaccg ggctcagagt cctcgtcgtc
     1501 gtagtcgtcg tagtcctcgt cgaagtcctc gatcaggccg tcctcgacga tcatctcgtc
     1561 gaactggccg tcgaggccga gagcggatgc tgcgacgtcg gcgcgctcgg tccagaacgc
     1621 cgcctcgtcg gtgcgtccga ggtcctcgag caccgccgcg cgcgccgaga agagggcggg
     1681 gctccactcg aaggcgcgat cggggtcgag ctcggggatc tcgagttcgc cgagtgcgag
     1741 ctcggtctct ccccggtcga ggcgggcgcc ggacatggcg atcgccagtg cgacgcggac
     1801 ctcgggttcg agcgccgcac ggtccacggc gcggccctcc tcgagggcgc ggtcgggacg
     1861 accgacgccg cgctcgctgt cgacgatcag ggcgatcatg tcgtccttgc cggagagacg
     1921 ccggtaggtg cgaagctccc gcagggcgag ggcgaagtcg ccgatgccgt atgcggtgat
     1981 cgcgagagtg gagcgcacca cggggatgcg gcccgcacga cgggatgccg ccagggcatg
     2041 cgcgtgcgcg cgctccggct cctcgtcgat cagctgagcg gccatcgcca ggtggcgggc
     2101 cgtctggtcg gcgttctcct tgctgagggt cttcagttcg ttgcgcgccg aggggtgcag
     2161 gtcgcgcgcg gtgatctcct cggggatcgg cggctcctcg atgcgctcgc gcaccggcgc
     2221 gaattcgttg atcggacgat cggtgccccc gcgcggacgg tcccggtagg acggacgctc
     2281 gccgcggtca tggtcgcgat ctccgaagga gcggcgctcg ccctgcgggc ggtcaccgta
     2341 gggacgacgc tcgccctggg gccggtcacc atacgggcga cgctcgccct ggggccggtc
     2401 accacggaac ggtcggtctc cctggctacg gtcgcggtca ccatacgggc gacgctcacc
     2461 ctgcgggcgg tcaccatacg ggcgacgctc accctgcggg cggtcaccat acgggcgacg
     2521 ctcaccctgc gggcggtcac catacgggcg acgctcgccc tgcgggcggt caccatacgg
     2581 gcgacgctcg ccctgcgggc ggtcaccata cgggcgacgc tcgccctggg gacggtcgcc
     2641 gcggtacggg cggtcgccct ggttgcggtc gcgatcgccg aaggagcggc gctcgccctg
     2701 gggacggtca cggtcaccgt agggacgacg ctcgccctgc gggcggtcac cgtagggacg
     2761 gcgctcaccc tgcggccggt caccgtaggg acggcgctcg ccctgagggc ggtcaccata
     2821 cggacgacgc tcaccctgag ggcggtcccg atcgccgaag gagcggcgct cgccctgagg
     2881 gcggtcacca tacggacgac gctcaccctg gccgcgatca cggtcaccat gagagcggcg
     2941 ctcgccctgg ttgcgatcgc cgtacggtcg gcgctcaccg tcacgggagc cgccggcatc
     3001 gttgcgctgg ggcgacctcg aggaggagtc cgagtaacgc ggcttgcgct cggagcccga
     3061 acgtcgatcg cgcgcgccct caccgtctcg gcggtcccgc tgctcatccg acattctgac
     3121 tccctttttg cttgttaacg caagatggcc acccaaccct gggtggccat cttacttaag
     3181 agaagtccgg cggtgtccta ctctcccaca gggtcccccc tgcagtacca tcggcgctgt
     3241 gaggcttagc ttccgggttc ggaatgtgac cgggcgtttc cctcacgcta tggccgccga
     3301 aacactattg acatgtatca g
//
LOCUS       DATMSX010000057         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_171406,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000057 DATMSX010000000
VERSION     DATMSX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171406"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /gene="uvrA"
                     /locus_tag="VN041_01675"
     CDS             <1..2008
                     /gene="uvrA"
                     /locus_tag="VN041_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWU27787.1"
                     /translation="TRMSVDVDLMLGDEELSIREGVILPWTTQGKGLFQYYERLLEGL
                     ARDLDFSLDTPWNQLHSDVREAVLRGDNYKVSVKWKNRYGREMRYTSGFEGVVPYIER
                     QYLQAESDTQRGRWGEYLREVPCPVCDGARLKPEVLAVKVHGHSIAEVSSLSLADASR
                     FMHTLELTEREAKIAAQVLREIRLRLEFLLQVGLSYLNLGRSAGSLSGGEAQRIRLAT
                     QIGSGLTGVLYVLDEPSIGLHQRDNRRLIDTLVRLRDLGNTLIVVEHDEETIEAADWV
                     VDIGPGAGVNGGAVVHSGPYSALLAETSSMTGDYLAGRREIATPKKRRKIDRKRMLSV
                     VGARANNLKNVTADFPLGVLTAVTGVSGSGKSSLVNDILYQVLAGRLNGARTVPGKHT
                     RVTGLDNLDKVVHVDQAPIGRTPRSNPATYTGVFDRIRTLFSETPEAKVRGYQPGRFS
                     FNVKGGRCDACSGDGTIKIEMNFLPDVYVDCEVCHGKRYNRDTLAVHYKGKNIAEVLE
                     MPIAEAAEFFEPIQAIHRYMKTLVDVGLGYVRLGQAATTLSGGEAQRVKLATELQRRS
                     NGRSIYVLDEPTTGLHFEDVRKLLEVLNGLVDKGNTVIVIEHNLDVIKSSDWVIDLGP
                     EGGSGGGEILATGTPEQIADVEESHTGRFLAEILGEGRAARKAG"
     gene            2008..>3458
                     /gene="uvrC"
                     /locus_tag="VN041_01680"
     CDS             2008..>3458
                     /gene="uvrC"
                     /locus_tag="VN041_01680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWU27788.1"
                     /translation="MADVLPYKPRTGEIPTDPGVYRFRDADGRVLYVGKAKNLRQRLS
                     NYFAPLRTLHERTRRMVTTAASVEWTVVATDVDSLQLEYMWIKEFDPPFNVRYKDDKS
                     YPFMAITLADEAPRVIVTRNRKIPGARYFGPYPKVWAVHDTIDLMVKAFPIRTCSDAS
                     YKRAMQTGRPCFPGQIGKCGGPCSMTCTIEEHRAMVDDFVAFMAGGDERFTKDLTRRM
                     LAASAAMDYEAAAKYRDKLASIEAVLGKSALVLPADEDADLFGLAEDELSATVHHFVI
                     RGGRVRGVRSLAIDKEIDISGAELVDQVLQRVYGDAVDIPRRVLVPVLPDDAAELEQW
                     LRERRGKRVEIAVAHRGQRAELMRTATLNAQQALLRHKTRRTSDYVARTQALTDLQEA
                     LGMSEAPLRIECYDISHLSGTNVVASMVVFEDGLPRKDQYRSFNIAETTDDTDSMYQV
                     LMRRLARVDDPVPADAADAQADGSGAGDPEGDVV"
BASE COUNT          597 a         1190 c         1187 g          484 t
ORIGIN      
        1 gacccggatg tccgtggacg tcgacctcat gctcggcgac gaggagctgt cgatccgcga
       61 gggagtcatc ctgccctgga ccacgcaggg caaggggctg ttccagtact acgagcgcct
      121 gctcgagggt ctcgcacgcg acctggactt ctcgttggac acgccctgga accagctgca
      181 ctccgacgtg cgcgaggcag tgctccgcgg tgacaactac aaggtcagcg tcaagtggaa
      241 gaaccgctac ggacgcgaga tgcggtacac ctccggcttc gagggcgtcg tgccctacat
      301 cgagcgccag tacctgcagg cggagtcgga cacgcagcgc ggacgctggg gcgagtacct
      361 gcgcgaggtc ccgtgcccgg tctgcgacgg cgctcgcctc aagcccgagg tgctggccgt
      421 caaggtgcac ggtcactcga tcgccgaggt ctcgagcctg agcctggccg acgccagccg
      481 cttcatgcac accctcgagc tcaccgagcg ggaggccaag atcgcagccc aggtgctgcg
      541 cgagatccgg ctgcggctgg agttcctgct gcaggtgggc ctctcctacc tgaacctcgg
      601 ccgctccgcc gggtcgctgt cgggcggtga ggcgcagcgc atccgcctgg cgacgcagat
      661 cggctccggc ctgaccggcg tgctctacgt gctcgacgag ccgtcgatcg gcctgcacca
      721 gcgcgacaac cgccggctga tcgacaccct cgtccgcctg cgcgatctcg gcaacacgct
      781 gatcgtggtc gagcacgacg aggagacgat cgaggcggcc gactgggtgg tcgacatcgg
      841 accgggtgcc ggcgtcaacg ggggagcggt cgtgcactcg ggcccctact ctgcgctgct
      901 cgccgagacc tcgtccatga caggcgacta cctcgccggc cgacgcgaga tcgccacgcc
      961 gaagaagcgc cgcaagatcg accgcaagcg gatgctgtcg gtcgtgggcg cgcgcgccaa
     1021 caacctgaag aacgtgaccg ccgacttccc gctcggggtg ctcaccgccg tgacaggcgt
     1081 gagcggctcg ggcaagtcgt cgctggtcaa cgacatcctg taccaggtgc tcgccgggcg
     1141 tctcaacggc gcccgcaccg tgccgggcaa gcacacccgc gtcaccggac tcgacaacct
     1201 cgacaaggtg gtgcacgtcg accaggcgcc gatcggccgc acgccgcgct cgaacccggc
     1261 cacctacacg ggcgtcttcg accgcatccg cacgctgttc agcgagaccc cggaggcgaa
     1321 ggtgcgcggc taccagcccg gccggttcag cttcaacgtc aagggcgggc gctgcgacgc
     1381 ctgctcgggc gacggcacga tcaagatcga gatgaacttc ctgcccgacg tgtacgtgga
     1441 ctgcgaggtg tgccacggca agcggtacaa ccgcgacacg ctggccgtgc actacaaggg
     1501 caagaacatc gccgaggtgc tcgagatgcc catcgccgag gccgcggagt tcttcgagcc
     1561 gatccaggcg atccaccgct acatgaagac cctggtcgac gtcggcctgg gatacgtgcg
     1621 gctgggccag gcggccacga cgctctccgg cggcgaggcg cagcgcgtca agctcgccac
     1681 ggaactgcag cggcgcagca acggccgcag catctacgtg ctcgacgagc cgaccaccgg
     1741 cctgcacttc gaagacgtcc gcaagctgct cgaggtgctg aacgggctcg tcgacaaggg
     1801 caacacggtg atcgtgatcg agcacaacct cgacgtgatc aagtcgtcgg actgggtgat
     1861 cgacctcggc cccgagggcg gttccggcgg tggggagatc ctcgccaccg gcacgcccga
     1921 gcagatcgcg gacgtggagg agagtcacac ggggcgcttc ctcgccgaga tcctgggcga
     1981 gggccgggcc gcacgcaagg cgggctgatg gccgacgtcc tgccgtacaa gccgcggacg
     2041 ggggagatcc ccactgaccc cggcgtgtac cgcttccggg atgccgacgg gcgagtgctg
     2101 tacgtcggca aggcgaagaa cctccggcag cggctgtcga actacttcgc cccgctgcgc
     2161 accctgcacg agcgcacccg gcggatggtc acgaccgctg cttccgtgga gtggacggtc
     2221 gtcgccaccg acgtggactc cctgcagctg gagtacatgt ggatcaagga gttcgatccg
     2281 ccgttcaacg tgcgctacaa ggacgacaag tcgtacccgt tcatggcgat cacgctcgcc
     2341 gacgaggcac ctcgggtgat cgtcacgagg aacaggaaga tccccggcgc gcgctacttc
     2401 gggccctacc cgaaggtgtg ggccgtgcac gacacgatcg acctgatggt caaggcgttc
     2461 ccgatccgca cctgcagcga cgccagctac aagcgcgcca tgcagaccgg gcggccgtgc
     2521 ttccccggcc agatcggcaa gtgcggcgga ccctgctcga tgacctgcac gatcgaggag
     2581 cacagggcca tggtcgacga cttcgtcgcc ttcatggccg gcggcgacga gcggttcacg
     2641 aaggacctca cccggcggat gctcgccgcg tccgcggcga tggactacga ggcggcggcg
     2701 aagtaccgcg acaagctcgc atcgatcgag gccgtgctcg gcaagagcgc gctcgtgctc
     2761 ccggccgacg aggacgccga tctgttcggg ctggcagagg acgagctctc ggcgacggtt
     2821 caccacttcg tgatccgcgg aggtcgcgtg cgcggtgtgc gctcgctcgc catcgacaag
     2881 gagatcgaca tctccggagc cgagctcgtc gaccaggtgc tgcagcgcgt gtatggcgac
     2941 gccgtcgaca tcccgcgccg cgtgctcgtc cccgtgctcc cggacgacgc ggccgagctc
     3001 gagcagtggc tgcgcgaacg ccgcggcaag cgcgtggaga tcgcggtcgc gcacaggggg
     3061 cagcgcgccg agctcatgcg caccgccacg ctcaatgcgc agcaggcgct gctgaggcac
     3121 aagacccgac gcaccagcga ctacgtcgcg cgcacgcagg ccctcaccga tctgcaggag
     3181 gcgctcggca tgagcgaggc tccgctgcgg atcgagtgct acgacatctc gcatctgagc
     3241 ggcacgaacg tcgtcgcctc gatggtcgtg ttcgaggacg ggctgccccg caaggaccag
     3301 taccgctcgt tcaacatcgc cgagaccacg gatgacaccg actcgatgta ccaggtgctg
     3361 atgcggagac tcgcgcgggt cgacgacccg gtgccggcgg atgccgcgga cgcacaggcc
     3421 gacggctcgg gcgccggcga cccggagggc gacgtggt
//
LOCUS       DATMSX010000058        17472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_95580,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000058 DATMSX010000000
VERSION     DATMSX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95580"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VN041_01685"
     CDS             <1..571
                     /locus_tag="VN041_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWU27789.1"
                     /translation="SSSVRQVHDGLTLCAFGGAFAGARLDIGTRVLLDAMRELSLPVV
                     RPQVVDLGCGTGALAAAFALSHPDAVVIATDRSASAVRSARETMAANGIEGRVQVTLD
                     DAGSELPDASADLVLLNPPFHLGASVHEGAGQRLIQAAARLLKPGGEVWTVFNSHLDH
                     RRALSRDVGATEQVTRTPKFTVTRSVRRS"
     gene            922..2256
                     /locus_tag="VN041_01690"
     CDS             922..2256
                     /locus_tag="VN041_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HWU27790.1"
                     /translation="MSEIALEAHHLYKVFGRNPQQAVKRLKTGESRTEVLDAGTAAVI
                     DASFTVKRGEIFVIMGLSGSGKSTIIRMLNGLHDITSGEVVIGGDDIAKANPAALREI
                     RRDRVSMVFQHFALLPHRTVAANVAYPLELKGVGKTERLAKAEEILALVGLEGWGDKL
                     PSELSGGMQQRVGIARALAADTDILLMDEAFSALDPLIRREMQEQLVELQAKLQKTIV
                     FITHDLNEAMFLGDRIAVMRDGRIVQIGTPEDILTDPANDYVEQFVQDVDRARVLTAS
                     NVMERPRPVVAETAGPRTALRQMRDAYMSATYVVGRDRKLIGIVTDRDAVKLVRKGET
                     SLSSILKPVPQAVDVDEVLMNLFIPSVESPLPLAVTDAGGRLVGVIPRVTLLAALGPG
                     PGATGELTLPLNPMPQAVIDEVLAEAQDAVESAAAAGPETHGSSTDDTEEVR"
     gene            2256..3212
                     /locus_tag="VN041_01695"
     CDS             2256..3212
                     /locus_tag="VN041_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HWU27791.1"
                     /translation="MDGFRIPLGKWVETGLNWLLDNLEWLFTFVSTVIGALVDGLSAL
                     LIQTPFPVTIVVFALIAWMLRSWKLALGTVISFVIILGLDLWTPAMQTMALVIVAAVI
                     AVIIAVPLGIWSARNDAVRAALKPILDFMQTMPAFVYLIPAIMFFGIGVVPGVVATVI
                     FALPPGVRLTELGIRGVDSETVEAGHAFGATPGQILRGVQLPLALPTIMAGINQLIML
                     ALSMAVIAGMAGADGLGKLVVQGISSMDIPMGVEAGLGIVLIAVFLDRCTAALAQPSP
                     SSLLGLLERTRQERRRREAAEAASATSAASELIAAGPRRAAP"
     gene            3283..4194
                     /locus_tag="VN041_01700"
     CDS             3283..4194
                     /locus_tag="VN041_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU27792.1"
                     /translation="MKKRHLTSILALTAAATVALAGCASGDTSKPGDKPAAGDDKGTI
                     TLGFLPSWTDGLSTAYLLQDQLEKMGYTVKMNTLTEAGPLYTGLSKGDVDVYPSAWPE
                     LTHKSYMDKFGDKIEDLGKYYDDAKLTIAVPEYTKIDSIADLKGNADMFGGKIIGIEP
                     GAGLTKQTQTKMLPEYGLDGEYELVTSSTAAMLTELKKATDAKRDIVVTLWRPFWAND
                     AYPVKDLKDPKGAMGKPEGLHFLGTKGFSEKNADAAEFIKKIKLTDEQYGALEGLVTS
                     DEYKDKSAEAVDKWISEYADQIDWLQK"
     gene            4378..5874
                     /locus_tag="VN041_01705"
     CDS             4378..5874
                     /locus_tag="VN041_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HWU27793.1"
                     /translation="MDGFAAIDFGFAREVVQRGIAALYLVAFLSSLNQFRVLLGKRGL
                     LPAPELLSWATSSKARRRMLRPTLFTRIRYTDRRLAALCWTGMAISALLVVGIPQLGP
                     PWVPMIAFLLLWGGYMSVVNIGQTFYGFGWEMLLLEAGFLAAFLGSRDQPPPTVVIVL
                     LWWLVFRLEFGAGMIKIRGGREWRDLTALTYHHETQPMPGPLSRQAHLLPRWFHKGEV
                     LGNHFAQLVVPFFLFAPILGLFRRWAPELVEGPQIVGTIAAAIVILTQLWLIVTGNFA
                     WLNWATVVIAFSAVSVPFGRPLSWAAEPVEAPQRLEPIDPYWIVITSAVGILYLAISW
                     PALRNLFAHRQLMNASFNRWQLANAYGAFGTVTKQRIEFVVEGTTDADPDAARWREYE
                     FKGKPGDLRRIPRQFAPYHLRLDWLMWFLPLGRSLEDWFAMFLVRLLEADPPTLRMLR
                     HDPFAGERPRWVRVVAYHYRFTTRAEHRASGDVWARERQGLVVRPLGG"
     gene            complement(5985..6926)
                     /locus_tag="VN041_01710"
     CDS             complement(5985..6926)
                     /locus_tag="VN041_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU27794.1"
                     /translation="MDDTPRIGIVGAGGIAPPHIEGWLALGAEVDILGRTGADALAER
                     YGLRVVDALDELIDACGIIDIVSPTATHLDIAKAAFARGRHVVCEKPLAVTVADARAI
                     AEAADEAGVRVFPAHVVRYFSGYEQVHEAVGGVGTLLELTFRRTVAAPASAWFYSQDA
                     GGGLIRDLMIHDLDQALWLAGPVVEVSATQDPPAIDGVVQPPVSAHVALTHANGAISH
                     VRADWFGPDTRFRSSASVVGSAGELSVDADELSGPREGYLPPASAVDPYRAQLADFLD
                     AIRTGRDARVTPADGVAAVALVEAAYASLATGHPVLL"
     gene            complement(7005..7787)
                     /locus_tag="VN041_01715"
     CDS             complement(7005..7787)
                     /locus_tag="VN041_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27795.1"
                     /translation="MTAAARMLLRGFSELGATDTMCHLGQWPYRLSAFADAGTLREYG
                     RRHGLHAIWVSHLAALFGFDTRTGNEAVLSACAGEPLLRPFAVIDPSETGWREELEWA
                     ADVGAQGVRVAPGMHHYDVALAAEVIDAAARRDLLVQVIVRLDDARVRHPSSPARDLA
                     VRDLAELVRSRPSSPLHLSGLNLAEATEFSRHLGDDIPPGVRLDMWHLNGPTGVVSDP
                     GAPLGDRRVFGSGFPVQTPEATMLQIASAGLPADRLRAIVTP"
     gene            complement(7784..8533)
                     /locus_tag="VN041_01720"
     CDS             complement(7784..8533)
                     /locus_tag="VN041_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU27796.1"
                     /translation="MIGIDAHTHLHDGGFDYQIIEEGRMLGIDLFLCSNIGDFVAHPS
                     IDEVREMNRALVRELDRLPDHLRGYCYVNPRYGRDTMDDLRRNVEEHGMVGIKLWIAT
                     LADDPLTDPILEYAAEHRLIVLTHAWRKTVGGFPYESTAEHVARAARRHPGTRFLMAH
                     LGGQADSAINIIREHPNVAVDTSGTIIGTGEVALAVQRLGTDRVVFGSDLPHVDLSVN
                     VGKVLGAGLNEDAADLVFGGTVAGWLEEVRR"
     gene            complement(8530..9744)
                     /locus_tag="VN041_01725"
     CDS             complement(8530..9744)
                     /locus_tag="VN041_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU27797.1"
                     /translation="MAASWRDRALAVMPIGSSTNSKAPTLVPDEPEVIVRGSGCRVWD
                     DRGREFIDYRCALGPITLGYADDRVDAAIRAQLADGILFGHPHPLETTTAELFCDIVP
                     GAEAARFLKTGGEALAALIRIARAHTGRDRIVQIGYNGWLNSLASGGRVLPGSTSSTV
                     QGVPGALSALHHAVEWDDRAALDGLFAARGSEIALILVAADYPSIPEAGDFYRYLREL
                     ADAHGALLGYDEMVTGFRVALGGMAEFTGVLPDLSVFGKGIANGMPLSVYCGRRDVLS
                     VLDRGEVVVSSTYGGETLSLAAATATMTALRDEDVLSRLRASGRRLQEGVNAEFAAAG
                     VGIRLVGHPTCPQFVADDDAVLQAFMRLAFRHGISLYRVAYVNAAHTEADIDLTVERL
                     RAALEDLAGETA"
     gene            complement(9789..10814)
                     /locus_tag="VN041_01730"
     CDS             complement(9789..10814)
                     /locus_tag="VN041_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU27798.1"
                     /translation="MTAVRPLRVAVLSFAHTHALSYVQALRAMPGIELIATDPDGAAA
                     PDDAPRGAELAAELGVEYVETYEQAFAWNPDAVVIAAENSRHRALVEQAAAAGAHVLC
                     EKPLATSVEDALAMREACERAGVILMVAYPVRFAPSFRDALAQIRSGGLGRVLSVTGV
                     NNGKLPQDRAWFTDPQLAGGGALVDHVVHCADLLDELLGERVRTVRAVSNGTLYADRD
                     LAVETGGMVTLQYPSGVIASIDCSWSWPVSSPTWGGLTLQVVAEHGTVTVSPFTQGVA
                     GHDAHGETWDPVGLDLDALLLAEFVAAVRDGRQPQPDAGVGVRTVEIVKAAQASAARG
                     GEVVTLV"
     gene            complement(10811..11806)
                     /locus_tag="VN041_01735"
     CDS             complement(10811..11806)
                     /locus_tag="VN041_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU27799.1"
                     /translation="MSTRRVGLIGAGGISRVHADAWSALGAEGYVTSLQGGEEIAAEY
                     GFTFVEDVDALIEAVDIVDILTPSSTHADLALRAIARGRDVICEKPLADTAENAARVV
                     QAAEEAGVRLFPAHVVRYMGEYERIKAGIVSGRIGEVAVQRFARAGAAPQSPWFFTES
                     TGGGVIRDLMIHDIDQAIWFAGPVASVYAVQNPPTVDDVVPAPVSSHVVLTHENGVIS
                     HLHASWVAAGMPFRTSVEVSGSSGRLRYDSAEDRVSRTDTVITAASTDYLPPMSPQES
                     PYYAEIADFMTALDGAREARITPADGVAAVAVAEAAYASIATGAPIALKTLEVVR"
     gene            complement(11803..12975)
                     /locus_tag="VN041_01740"
     CDS             complement(11803..12975)
                     /locus_tag="VN041_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU27800.1"
                     /translation="MSRHSSLPIAVRAALHIRDRGTATVTEIAQTLELSRTSVENAMG
                     VLADIDLIADAPVATGRGAGRPARRFAFQNRTGHVVGVDIGIASVRVVIADLSGQVVL
                     HRGFDGVTHSRDAVDKLATVIDDIRTTLHEAGIPRSRVRAIGVSLPGIVDDNGRVTTS
                     VVIPEWSGVDIGSQLSDAFGCPVSVDNGVRLAAVAEHHLGVAQLVDDVLYLSVGNRIA
                     MGLILGGRPRRGIHNAAGDIGRLAFPGLVTETGQLTWRTGESAEDVFIRARNGDVDSQ
                     QELTGFIDQIARGIATLALTVDPAMIVIGGGLSAAHEQFLDPLRPALSTHIGLPFAVP
                     VVGARLGAEAASHGAVVFAFRRHAAAIYGIEGMPVPPITPLPAGVAAVADLQEETA"
     gene            13169..14404
                     /locus_tag="VN041_01745"
     CDS             13169..14404
                     /locus_tag="VN041_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU27801.1"
                     /translation="MRAKRFAGVIAGLATAGLIAGCAAGGGDAAKGDETVTLWVYPVI
                     ADEAKHKAFWDETVSAFEKENTDIKVKYEIFPWANRDESLQTAIAANKGPDLVYLIPD
                     QLMAYQKSIEPMDKYLSKDRKSEILPNVTKSVTIDGHMMGAPLLTSATPLLCNAAVFK
                     EAGVTEYPETWEDALRIAPKFTEKGLHLFNYTASAEATLNMTFYPLLWQAGGAVYGKD
                     GSVAFDGKAGTEALTFLSDLAKMGALDPDALTSLPATEQSGIATGKTACSWVNSVTEL
                     APFWGEENVKVLAPLKDKKSITYGTVGSLSMLKGSKVKDAAGKFADFATSAEVLKPYL
                     TEAGFFSALDTAPLYPADSLQGQVEKYVSSTTVGELAPSSRALMGVVSPEIQAALLGQ
                     KSPKQALSDAAKAAAPLLK"
     gene            14464..15396
                     /locus_tag="VN041_01750"
     CDS             14464..15396
                     /locus_tag="VN041_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU27802.1"
                     /translation="MTTAPPATASPTQSAPAKRRRTTTRLLARREARIAFLFVLPAFL
                     LFIAFRFGPAIAGVALSFFDYDISGEIDWRGLDNFQRLFEDALFWRALGTTLLYTVFA
                     VPISLALSTVMALGVRRSFRGSRFFRSIFFLPVITSLVLAGAIFIWIFSRNGPWSTLM
                     SPLGLGGSWLASTVLVVPAIIIVGVWSRFGYGMMIMIAAMQDIPRELEEAALMDGANA
                     WQRFRWVILPFLRPTVFFLAVIETTAAFQVFDVIYVMTQGGPANASYSLVYLIYDQGF
                     KYFEYGYAAAAGVALFVMTLAVALIQRLVIGKQK"
     gene            15393..16265
                     /locus_tag="VN041_01755"
     CDS             15393..16265
                     /locus_tag="VN041_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU27803.1"
                     /translation="MTAVTTNAQLRQRSYKALEPTRGGLVLRWVWLSIAAVFSFFPFY
                     AMVVLSLKPGAVVNFPQSLLPWGDLSLSAYEQVLSGQNILLWLFNTLVYALVSVVAVL
                     FLSALAGYAFAKKRFAGKEVMFWSFLSMVMVPFHVTLIPTFLLIANLGGVNEYWGLIL
                     PTLANAQAVFLMRQFIEGLPDELFEAARIDGASELRIFLRIVLPLCKPVLATLGIFVF
                     LWHWNDFLWPLIIAKSNEMFTLTVGISSLQQQNVPLSVMLAGSVVALLPIFMAYLIAQ
                     RYVQEGVTGSGIKG"
     gene            16271..17314
                     /locus_tag="VN041_01760"
     CDS             16271..17314
                     /locus_tag="VN041_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HWU27804.1"
                     /translation="MSDVTTKSFTSEAELEQALTAPSDALIEDFSRTSGDLVILGAGG
                     KMGPTLAVLARRAMDAAGRNADTVYAVSRFGDPAVTERLRSANVEVVPFDLIENDDFS
                     SLPDAPNVVFMVGAKFGAATSPSWAWEVNAALPDRVARRYRDSAISVLSTGNVYPFLP
                     ASSGGASEEVTPAPIGEYAQSCLGRERVFEFGAQQRGTKVALIRLNYAVDLRYGVLAD
                     IGSAVHAGEPVSLATANVNVIWQGYANEVVLRSLLHATSDVFTINLTGPELLSVESIA
                     RRFGALFDSEVHLVDEPQPTALLSDARRCMGLFGYPSVPAETLIELQAAWIRDELPMT
                     AKPTKWAVRDGKF"
BASE COUNT         2701 a         6082 c         5968 g         2721 t
ORIGIN      
        1 ttcttcatcg gtccgccagg tgcacgacgg tctcacgctg tgcgcgttcg gcggcgcgtt
       61 cgccggcgcg cggctcgaca tcggcacgcg cgtgctgctc gatgcgatgc gggagctctc
      121 gctcccggtc gtccgtccgc aggtcgtcga cctgggctgc gggacgggtg cactcgccgc
      181 ggcattcgcc ctgagccatc cggatgccgt ggtcatcgcg accgaccgct cggcctcagc
      241 cgtccgctcc gcccgcgaga ccatggccgc caacggcatc gagggccgcg tccaggtcac
      301 gctcgacgac gccggctccg aactgccgga tgcctcggcc gatctggtcc tgctgaaccc
      361 gcccttccac ctcggcgcga gcgtgcacga gggcgccggg cagcgcctca tccaggccgc
      421 cgcccggctg ctgaagcccg gcggcgaggt gtggacggtg ttcaactcgc acctcgacca
      481 tcggcgtgcc ctcagccgcg acgtcggggc gacggagcag gtcacccgca cccccaagtt
      541 caccgttacc cgcagcgtcc gccgcagctg aatcccgtcg aatcgccacc ggaaacctca
      601 cagcgatcga atttccgaca cgcgcataac ccccggtgag gccatgaaac cgaaacgcgg
      661 atttgccacc cgatcagcca ttcgttagcg tggacaggtg gtcgagggac cacaacacca
      721 gtccgcggcc gcgtccttct ttcgactcga gctgtacacg aagaggcgcg ggcgtacggt
      781 cgatgttctt tgcggggatc cgaaatcccc gaccgcgccg ccagtacagc gattgtcgaa
      841 acggccctgt gatcatcacc cgggcgttac cgaagccggc tcacacgcac cctggcgaca
      901 ggtgagccgc gcgggggaaa cgtgtccgaa atcgctcttg aagcgcacca tctctacaag
      961 gtcttcggcc gcaatccgca acaggcggtc aagcggctca agaccgggga atcccgaacc
     1021 gaagtactgg acgccggcac ggcagccgtg atcgacgcga gcttcaccgt gaagcgcggt
     1081 gagatcttcg tgatcatggg gctgtccgga tccggcaagt ccaccatcat ccgcatgctc
     1141 aacggcctgc acgacatcac ctccggcgag gtggtcatcg gcggcgacga catcgccaag
     1201 gcgaacccgg ccgccctgcg cgagatccgc cgcgaccgcg tctcgatggt cttccagcac
     1261 ttcgcgctgc tgccgcaccg cacggtggcc gccaacgtgg catacccgct cgagctcaag
     1321 ggcgtcggca agaccgaacg gctggccaag gccgaggaga tcctcgcgct ggtcgggctg
     1381 gagggctggg gcgacaagct gcccagcgag ctctccggtg gcatgcagca gcgcgtcggc
     1441 atcgcccgcg cgctcgccgc ggacaccgac atcctgctga tggacgaggc cttcagcgcg
     1501 ctcgatcccc tgatccgccg cgagatgcag gagcagctcg tcgagctgca ggccaagctg
     1561 cagaagacca tcgtgttcat cacgcacgat ctcaacgagg ccatgttcct cggcgaccgg
     1621 atcgccgtga tgcgcgacgg ccgcatcgtg cagatcggca cgcccgagga catcctcacc
     1681 gacccggcca acgactacgt cgagcagttc gtgcaggacg tcgaccgcgc ccgtgtgctc
     1741 accgcgtcca acgtcatgga gcgtccgcgt cccgtggtcg ccgagaccgc cggtccgcgc
     1801 acggcgctgc gccagatgcg cgacgcctac atgtcggcca cctacgtggt cggccgcgac
     1861 cgcaagctga tcggcatcgt caccgaccgg gatgccgtga agctggtccg caagggcgag
     1921 acctcgctgt cgagcatcct caagccggtg ccgcaggcgg tggacgtcga cgaggtgctg
     1981 atgaacctgt tcatcccctc ggtcgagtcg ccgctgcccc tcgcggtgac ggatgccggg
     2041 ggccgcctgg tcggcgtcat cccgcgcgtc accctgctcg ccgcgctcgg ccccggcccg
     2101 ggtgccaccg gtgagctgac ccttccgctg aacccgatgc cccaggccgt gatcgacgag
     2161 gtgctggccg aggcgcagga tgccgtggaa tctgccgccg cggcaggccc ggagacgcat
     2221 gggtcgagca ctgacgacac cgaggaggtg cgctgatgga tggattccgt attccgctcg
     2281 ggaagtgggt cgagaccggt ctgaactggc tgctcgacaa cctggagtgg cttttcacgt
     2341 tcgtgtcgac ggtcatcggc gcgctggtcg acggactgtc ggccttgctg atccagactc
     2401 cgttccccgt cacgatcgtc gtcttcgcgc tgatcgcgtg gatgctgcgc tcgtggaagc
     2461 tcgcgctggg cacggtgatc tcgttcgtga tcatcctggg gctcgacctg tggacaccgg
     2521 ccatgcagac catggcgctc gtcatcgtcg ccgccgtgat cgccgtgatc atcgccgtgc
     2581 cgctgggcat ttggtcggcc cgcaacgacg ctgtgcgtgc ggcgctcaag ccgattctcg
     2641 acttcatgca gaccatgccc gcgttcgtgt acctgatccc ggcgatcatg ttcttcggca
     2701 tcggcgtggt gccgggcgtc gtcgcgaccg tgatcttcgc gctgcctccc ggggtgcgcc
     2761 tgaccgagct cggcatccgc ggcgtcgaca gcgagaccgt cgaggccggg cacgcgttcg
     2821 gcgcgacccc cggtcagatc ctgcgcggcg tgcagctgcc gctggcgctg cccaccatca
     2881 tggccggcat caaccagctg atcatgctgg cactgtcgat ggccgtcatc gccggtatgg
     2941 ccggtgcaga cggactcgga aagctcgtcg tgcagggcat ctcgtcgatg gacatcccga
     3001 tgggcgtcga ggccggtctc ggcatcgtgc tgatcgccgt cttcctcgac cgctgcaccg
     3061 cggccctcgc acagccctcg ccgtcgtccc tgctcggtct tctcgagcgc acccggcagg
     3121 agcgtcgtcg ccgcgaggcg gcggaggcgg cgtccgcgac atcggcggca tccgaactca
     3181 tcgcggccgg gccccgccgg gccgctccct gatcccccag ctccacttcg caccgaatca
     3241 catacagcac gcacgagtca tgcatcacga aaggcaatac acatgaagaa gcgacacctc
     3301 accagcatcc tggcgctgac cgccgccgca accgtggccc tggccggctg cgcgagcggc
     3361 gatacgtcga agcccggcga caagcccgcc gcgggcgacg acaagggcac catcacgctc
     3421 ggcttcctgc ccagctggac cgacggtctg agcaccgcct acctgctgca ggaccagctc
     3481 gagaagatgg gctacacggt caagatgaac acgctcaccg aggccggccc gctctacaca
     3541 ggcctgtcca agggcgacgt cgacgtctac ccctcggcct ggcccgagct gacgcacaag
     3601 tcgtacatgg acaagttcgg cgacaagatc gaggacctcg gcaagtacta cgacgacgcc
     3661 aagctcacga tcgcggttcc cgagtacacg aagatcgact cgatcgccga cctcaagggc
     3721 aacgccgaca tgttcggcgg caagatcatc ggcatcgagc cgggcgccgg cctgaccaag
     3781 cagactcaga cgaagatgct cccggagtac ggcctggacg gcgagtacga gctcgtcacc
     3841 tcgtcgaccg cggcgatgct caccgagctg aagaaggcga cggatgccaa gagggacatc
     3901 gtcgtcaccc tgtggcgtcc gttctgggcc aacgacgcat acccggtgaa ggacctgaag
     3961 gacccgaagg gcgccatggg caagcccgaa ggcctgcact tcctgggcac gaagggattc
     4021 agcgagaaga acgccgacgc cgccgagttc atcaagaaga tcaagctgac cgacgagcag
     4081 tacggggcgc tcgagggcct ggtcacctcc gacgagtaca aggacaagtc ggctgaggcc
     4141 gttgacaagt ggatctcgga gtacgccgac cagatcgact ggctgcagaa gtaggcattg
     4201 tctccacgag ggggaggggc gttctcgccc cctctgaatg ccgcggcccc tgctgagtta
     4261 tgaaactcag caggggccgc ggcgatggac gggggcgacg acggagcggg caggcgccgt
     4321 cgcagcgggc tgtcaaggcc tctgccgcgt caggccgcct cggcctagcc tggtcgcatg
     4381 gacgggttcg cggccatcga cttcgggttc gcgcgcgagg tcgtgcagcg cggcatcgcg
     4441 gcgctgtacc tcgtcgcgtt cctgtcgagc ctgaaccagt tccgtgtgct gctgggcaag
     4501 cgcggcctgc tccccgcccc cgagctgctg agctgggcga cctcgtcgaa ggcgaggagg
     4561 cggatgctgc gtccgaccct gttcacgcgg atccgctaca cggaccggcg cctggcggcg
     4621 ctgtgctgga cgggcatggc gatctcggcg ctgctggtgg tcggcatccc gcagctcggg
     4681 ccgccgtggg tgccgatgat cgccttcctg ctgctgtggg gcgggtacat gtcggtggtg
     4741 aacatcggcc agaccttcta cggcttcggc tgggagatgc tgctgctcga ggcgggattc
     4801 ctcgcggcgt tcctcggatc ccgcgatcag ccgccgccga ccgtggtgat cgtgctgctg
     4861 tggtggctgg tgttccggct ggagttcggt gcggggatga tcaagatccg cggtggacgg
     4921 gagtggcgcg acctcaccgc gctgacctac caccatgaga cgcagccgat gccggggccg
     4981 ctcagccggc aggcgcacct gctgccccgc tggttccaca agggggaggt gctgggcaac
     5041 cacttcgcgc agctggtggt gccgttcttc ctgttcgcgc cgatcctggg actgttccgg
     5101 cgctgggccc ctgagctcgt cgaagggcct cagatcgtcg gcacgatcgc ggcggcgatc
     5161 gtcatcctca cccaactgtg gctgatcgtg accggcaact tcgcctggct gaactgggcc
     5221 acggtcgtga tcgcgttctc ggcggtgtcg gtgccgttcg gccggcccct gtcctgggcc
     5281 gctgagcctg tcgaagcgcc gcagcgtctc gagccgatcg atccgtactg gatcgtgatc
     5341 acctcggcgg tcggcatcct gtacctcgcg atcagctggc cagcgctgcg caacctgttc
     5401 gcgcaccgtc agctgatgaa cgcgagcttc aaccgctggc agctcgccaa cgcctacggc
     5461 gccttcggca cggtcacgaa gcagcgcatc gagttcgtcg tcgagggcac gacggatgcc
     5521 gatccggatg ccgcccgctg gcgcgagtac gagttcaagg gcaagcccgg cgacctgcgc
     5581 cgcattccgc ggcagttcgc cccgtatcac ctgcggctgg actggctgat gtggttcctg
     5641 ccgcttgggc gctcactgga ggactggttc gcgatgttcc tcgtgcggct gctcgaagcg
     5701 gacccgccga ccctgcggat gctgcggcac gatccgttcg caggcgaacg cccccgctgg
     5761 gtgcgcgtgg tggcgtacca ctaccggttc accacgcgcg ccgagcaccg ggcatccggc
     5821 gatgtctggg cgcgcgagcg gcaggggctg gtggtgcgcc cgctcggcgg gtgaccgctc
     5881 gttcgccggc acaggtgcac gaccggggaa cgtctgcacg atcgaatcgg gcatccggcc
     5941 gtgcatccgt acccgggtcg tgcaggtgta cccggggcgg accctcagag caggaccgga
     6001 tgcccggtcg cgagcgacgc gtacgcggcc tccaccagtg cgacggcggc gacgccgtcg
     6061 gcgggcgtga cgcgcgcgtc gcgaccggtg cggatggcat cgaggaagtc ggcgagctgc
     6121 gcccggtacg gatccacggc ggatgccggt ggcaggtacc cctcccgtgg gccggacagc
     6181 tcgtcggcgt ccaccgacag ctcgccggcc gagcccacca ccgacgcgct cgaacggaac
     6241 cgggtgtccg gcccgaacca gtccgcgcgc acatggctga tggcgccgtt cgcgtgggtg
     6301 agtgcgacgt gtgccgacac cggtggctgc acgacgccgt cgatcgccgg cggatcctgc
     6361 gtcgcggaca cctcgaccac ggggcctgcc agccacagcg cctgatccag gtcgtggatc
     6421 atgaggtcgc ggatgaggcc gcctccggca tcctgcgagt agaaccaggc ggatgccggg
     6481 gccgcgaccg tccggcggaa cgtcagctcc agcagggtgc cgaccccgcc gaccgcctcg
     6541 tgaacctgct cgtatccgga gaagtagcgc accacgtgcg cggggaacac gcgaacgccc
     6601 gcctcgtccg cggcctccgc gatggcccgt gcgtcggcga cggtgacggc caagggcttc
     6661 tcgcacacca cgtgcctgcc gcgggcgaag gcggccttcg cgatgtcgag gtgcgtcgcg
     6721 gtggggctga cgatgtcgat gatcccgcag gcgtcgatca gctcgtcgag ggcgtcgacg
     6781 acgcggagac cgtagcgctc ggcgagggca tccgccccgg tgcggcccag gatgtcgacc
     6841 tcggcgccga gcgcgagcca tccctcgatg tgcggtgggg cgatgccgcc ggcgccgacg
     6901 atcccgatcc gcggagtgtc atccatacgg ccgagcctac gtcgcgggga aggctgctgc
     6961 tcggggaaac cggatgagcg tgctctcccg tctcctgcgg cggcctaggg ggtcacgatg
     7021 gcgcgcagcc ggtctgcggg gagtccggcc gacgcgatct gcagcatggt cgcctcgggc
     7081 gtctgcaccg ggaagccgct gccgaagacc cggcgatcgc cgagcggagc gccggggtcc
     7141 gacacgacgc cggtcggccc gttgagatgc cacatgtcca gccgcacgcc gggcgggatg
     7201 tcgtcgccga ggtgacggga gaactccgtg gcctcggcga gattcagccc gctgagatgg
     7261 agcggactcg acggccggga ccggacgagc tcagcgagat cgcggaccgc caggtctcgc
     7321 gcgggagagg agggatgacg gacgcgtgcg tcgtccagcc gcacgatgac ctgcacgagc
     7381 aggtcgcgtc gcgcagcggc atcgatcacc tcggcggcca gtgccacgtc gtagtgatgc
     7441 atcccgggcg ccacccgcac gccctgggcg ccgacatccg ccgcccactc gagctcttcg
     7501 cgccagcccg tctcgctcgg gtcgatcacg gcgaagggac gcagcagcgg ctcgccggca
     7561 caggccgaca gcacggcttc gttccccgtc cgcgtgtcga agccgaagag cgccgccagg
     7621 tgcgagaccc agatcgcgtg cagaccgtgg cgcctcccgt actcccgcag cgtgccggca
     7681 tcggcgaagg ccgaaagccg gtacggccac tggcccaggt ggcacatggt gtcggtcgcg
     7741 cccagctcgg agaagccgcg cagcagcatc cgcgccgccg ccgtcatctc cgcacctctt
     7801 cgagccaccc ggcgacggtg cctccgaaca cgagatccgc ggcatcctcg ttcagacctg
     7861 caccgaggac cttgcccacg ttcaccgaca ggtccacgtg cgggaggtcc gatccgaaga
     7921 cgacgcggtc ggtgccgagc cgctgcacgg cgagagcgac ctcgcccgtg ccgatgatcg
     7981 tccccgaggt gtccacggcc acattggggt gctcgcggat gatgttgatc gccgagtcgg
     8041 cctgtccgcc cagatgagcc atcaggaacc gggtgccggg atggcggcgc gccgcacgtg
     8101 cgacatgctc cgcggtggac tcgtacggga agcctccgac ggtcttgcgc catgcgtgcg
     8161 tgaggacgat cagccggtgc tcggcggcgt actcgaggat cggatcggtc aggggatcgt
     8221 cggcgagcgt ggcgatccag agcttgatgc ccaccatccc gtgctcctcg acgttccgcc
     8281 gcaggtcgtc catggtgtcg cggccgtacc gggggttcac gtagcagtac ccgcgcaggt
     8341 gatcgggcag ccggtccagc tcgcgcacca gtgcgcggtt catctcccgc acctcgtcga
     8401 tcgagggatg cgcgacgaag tcgccgatgt tgctgcagag gaagagatcg atgccgagca
     8461 tccgtccctc ctcgatgatc tgatagtcga atccgccgtc gtgcagatgc gtgtgcgcat
     8521 cgatgccgat cacgccgtct cccccgcgag atcctcgagg gccgcacgca gacgctccac
     8581 ggtcaggtcg atgtcggcct cggtgtgcgc cgcgttcacg tacgccaccc ggtacaggga
     8641 gatcccgtgc cggaacgcca gccgcatgaa cgcctgcagg acggcatcgt cgtcggcgac
     8701 gaactgcggg caggtcggat gcccgaccag gcggatgccg actccggccg cggcgaactc
     8761 cgcgttgacg ccttcctgca gacgccggcc ggacgcacgg agccgggaca gcacgtcctc
     8821 gtcgcgcagc gccgtcatgg tcgcggtcgc ggccgcaagc gagagcgtct cgccgccgta
     8881 cgtcgacgag accacgacct cgccccgatc cagcaccgag agcacgtccc ggcgcccgca
     8941 gtagaccgac agcggcatgc cgttggcgat gcccttgccg aacacggaca gatcgggcag
     9001 cacgcccgtg aactccgcca tcccgcccag cgcgacgcgg aatccggtga ccatctcgtc
     9061 gtacccgagc agcgcaccgt gcgcgtcggc gagctctcgc agataccggt agaagtcgcc
     9121 cgcctccggg atggacgggt agtcggcggc gaccaggatc agcgcgatct ccgagccgcg
     9181 cgcggcgaac agcccgtcca acgcggcacg atcgtcccac tcgacggcgt gatgcagagc
     9241 cgaaagcgca cccggcactc cctggacggt gctcgacgtg ctgcccggga gcacgcggcc
     9301 tccggatgcc agcgagttca gccagccgtt gtagccgatc tgcacgatcc ggtcccttcc
     9361 ggtgtgcgcg cgggcgatgc ggatcagcgc cgccagcgcc tcgccaccgg tcttcaggaa
     9421 gcgcgccgcc tccgcgccgg ggacgatgtc gcagaacagc tccgccgtcg tcgtctcgag
     9481 cgggtgggga tgcccgaaga ggatgccgtc ggcgagctgc gcgcggatcg ccgcgtccac
     9541 ccgatcgtcg gcgtatccca gcgtgatcgg accgagcgcg cagcggtagt cgatgaactc
     9601 gcgcccgcga tcgtcccaga cgcggcatcc cgaaccgcgg acgatcacct ccggctcatc
     9661 ggggaccagc gtcggcgcct tggagttcgt gctggagccg atcggcatga ccgcgagcgc
     9721 acggtcccgc cagctcgccg ccatgtccat gtcctcatct ctccttcggg atgctgcgtc
     9781 tgcgcgggtc agaccagggt gacgacctcg ccgccgcgtg cggccgaggc ctgcgcggcc
     9841 ttcacgatct ccaccgtgcg cacaccgacg ccggcatccg gctgcggctg ccggccgtcc
     9901 cggaccgccg ccacgaactc cgcgagcagc agggcgtcga ggtccaggcc caccgggtcc
     9961 caggtctcgc cgtgcgcgtc atgccctgcc acgccctgcg tgaacgggct gaccgtgacc
    10021 gtgccgtgct cggccaccac ctgaagggtg agtccgcccc aggtggggga ggagaccggc
    10081 cagctccagg agcagtcgat gctcgcgatg acgccgctgg gatactgcag ggtgaccatg
    10141 ccgccggtct ccacggccag gtcgcgatcg gcgtacagtg tgccgttcga caccgcccgc
    10201 accgtgcgga cgcgctcgcc gaggagctcg tcgagcagat cggcgcagtg cacgacgtgg
    10261 tcgaccagag cgccgccccc ggcgagctgc ggatcggtga accaggcgcg gtcctgcggc
    10321 agcttgccgt tgttcacgcc ggtgacgctc agcaccctgc ccaggccgcc gctgcggatc
    10381 tgcgcgagtg cgtcgcggaa cgagggcgcg aaacggaccg gataggcgac catcaggatc
    10441 acgccggcgc gctcgcacgc ctcgcgcata gccagggcat cctcgaccga cgtcgccagc
    10501 ggcttctcgc acagcacgtg cgcgccggcg gccgcggcct gctcgaccag cgcacggtgg
    10561 cgggagttct ccgccgcgat caccacggca tccggattcc acgcgaatgc ctgctcgtag
    10621 gtctccacgt actcgacgcc gagctccgcc gcgagctccg cgccgcgcgg ggcgtcatcg
    10681 ggagcggccg cgccgtcggg gtcggtcgcg atcagctcga tacccggcat ggcgcgcagc
    10741 gcctgcacat agctgagggc gtgggtgtgc gcgaacgaga gcaccgcgac gcggagcggg
    10801 cggacggcgg tcatcggacg acctccaggg tcttgagggc gatcggcgct cccgtggcga
    10861 tcgacgcata ggccgcctcg gccacggcca cggctgccac gccgtccgcc ggtgtgatcc
    10921 gcgcctcgcg tgcgccgtcg agcgccgtca tgaagtccgc gatctcggcg tagtaggggc
    10981 tctcctgcgg cgacatcggc ggcaggtagt cggtggaggc ggcggtgatg acggtgtcgg
    11041 tgcggctgac gcggtcctcg gcgctgtcgt accgcagtcg cccggacgag ccggagacct
    11101 cgacgctggt gcggaacggc atgccggccg cgacccagct ggcatgcaga tggctgatga
    11161 cgccgttctc gtgggtgagc acgacgtggg agctgacggg ggcgggtacg acgtcgtcga
    11221 cggtcggcgg gttctgcacc gcgtagacgg atgccacggg cccggcgaac cagatggcct
    11281 ggtcgatgtc gtggatcatc agatcgcgga tgacgccgcc gcccgtgctc tccgtgaaga
    11341 accagggcga ctgcggcgcg gcgccggcgc gggcgaagcg ctgcacggcg acctcgccga
    11401 tgcgccccga aacgatgccc gccttgatgc gctcgtactc gcccatgtag cgcacgacgt
    11461 gcgcggggaa gagccgcaca cccgcctcct ccgcggcctg cacgacgcgg gcggcgttct
    11521 cggcggtgtc ggcgagaggc ttctcacaga tcacgtccct tccgcgcgcg atcgcgcgga
    11581 gggcgaggtc ggcgtgggtg ctgctggggg tgaggatgtc gacgatgtcg accgcctcga
    11641 tgagggcgtc gacgtcctcg acgaacgtga agccgtactc ggcggcgatc tcctcgccac
    11701 cctggaggga ggtcacgtag ccctccgcgc ccagtgcgct ccaggcgtcg gcatgcacgc
    11761 gggagatccc tccggcgccg atgagtccga ctctccgtgt gctcacgctg tctcctcctg
    11821 aaggtctgcg actgcggcga ctcccgcagg aagcggtgtg atgggcggga ccggcatgcc
    11881 ctcgatcccg tagatggcgg cggcgtgccg gcggaaggcg aacacgaccg caccatgcga
    11941 ggcggcttcc gcccccagac gcgccccgac caccgggacg gcgaacggga gtccgatgtg
    12001 ggtggacagt gcgggccgca gcgggtcgag gaactgctcg tgcgcggccg acaggccgcc
    12061 gccgatgacg atcatggccg ggtcgacggt cagcgccagg gtggcgatgc cacgggcgat
    12121 ctggtcgatg aacccggtga gctcctgctg ggagtcgacg tcgccgttgc gggcgcggat
    12181 gaagacgtcc tccgcgctct cgccggtgcg ccaggtcagc tggccggtct cggtgaccag
    12241 accggggaag gcgagccggc cgatgtctcc tgcggcgttg tggattcctc gccgcggtcg
    12301 cccgccgagg atcaggccca tggcgatgcg gttgccgacg gacaggtaca gcacgtcgtc
    12361 gaccagctgc gcgacgccga ggtggtgctc ggcgacggcg gcgagccgga cgccgttgtc
    12421 gacggagacc gggcagccga aggcatccga gagctgggag ccgatgtcga cgcccgacca
    12481 ctccgggatg accaccgagg tcgtcacgcg gccgttgtcg tcgacgatgc cggggagcga
    12541 gacgccgatg gcgcgcactc tcgaccgcgg gatgcctgcc tcgtgcagag tggtgcggat
    12601 gtcgtcgatc acggtggcga gcttgtcgac ggcgtcgcgc gagtgggtga cgccgtcgaa
    12661 gccgcggtgc agcacgacct ggccgctgag gtcggcgatg acgacgcgca ccgacgcgat
    12721 gccgatgtcg acgccgacca catgaccggt gcggttctgg aaggcgaagc ggcgggccgg
    12781 gcggcccgca ccacgaccgg tcgccaccgg tgcgtcggcg atcagatcga tgtcggcgag
    12841 aacgcccatc gcgttctcca ccgaggtgcg ggagagctcg agagtctgtg cgatctcggt
    12901 gacggtcgcg gtgccccggt cacggatgtg gagggcggcc cgcacggcga tgggcaggct
    12961 gctgtgtcgt gacacgggct gctcctctcg tcatcgatgg cgtgcgactc aaattagcac
    13021 actgcattcg cggaatgaca gggggatctt ctccgggttc agtcgctaga aaatcaacat
    13081 ctggcgagaa cttgaagatc gtgcttgact aattatccca cttgactgtc ataatgacat
    13141 cacccgcacg gttcagcaaa ggagcatcat gcgcgcaaag agattcgccg gcgtcatcgc
    13201 cgggcttgcc accgccggcc tcatcgccgg ctgcgcggcg ggagggggcg acgccgcgaa
    13261 gggtgatgag accgtcaccc tctgggtcta ccccgtcatc gccgacgagg ccaagcacaa
    13321 ggccttctgg gacgagaccg tctcggcgtt cgagaaggag aacaccgaca tcaaggtcaa
    13381 gtacgagatc ttcccctggg cgaaccgcga cgagagcctg cagaccgcga tcgccgccaa
    13441 caagggtccg gatctcgtct acctgatccc cgaccagctg atggcctacc agaagtcgat
    13501 cgaaccgatg gacaagtacc tcagcaagga ccgcaagtcc gagatcctcc cgaacgtgac
    13561 gaagtcggtc acgatcgacg gccacatgat gggcgccccg cttctgacca gcgcgacccc
    13621 cctgctctgc aacgcggcgg tcttcaagga ggccggcgtc acggagtacc ccgagacgtg
    13681 ggaggacgcg ctccgcatcg cgccgaagtt caccgagaag gggctgcatc tcttcaacta
    13741 cacggcatcc gccgaggcca ccctcaacat gaccttctac ccgctgctct ggcaggcggg
    13801 cggcgccgtg tacggcaagg acggatccgt cgcgttcgac ggcaaggccg gcaccgaggc
    13861 gctcacgttc ctctccgacc tcgccaagat gggcgcgctc gacccggatg cgctgacctc
    13921 gctccccgcc accgagcaga gcggcatcgc gaccgggaag accgcatgct cgtgggtcaa
    13981 cagcgtcacc gagctcgccc cgttctgggg tgaggagaac gtcaaggtgc tcgctccgct
    14041 gaaggacaag aagagcatca cctacggcac ggtcggatcg ctgtcgatgc tcaaggggtc
    14101 gaaggtcaag gatgccgcgg gcaagttcgc ggacttcgcc accagcgccg aggtgctcaa
    14161 gccctacctg accgaggcgg gattcttctc cgcgctcgac accgcaccgc tctaccccgc
    14221 cgactccctg cagggccagg tcgagaagta cgtgtcctcg accacggtcg gcgagctcgc
    14281 gccgagctct cgtgccctga tgggcgtggt gtcgcccgag atccaggccg ccctgctggg
    14341 gcagaagtcg ccgaagcagg cgctcagcga cgcggctaag gccgcggctc cgctcctgaa
    14401 gtgacgatcc ggaggggcgg cggacacccc cgccgcccct ccccacgacg tgaggtcaag
    14461 gcaatgacta cagccccacc tgcgacagcg tctccgacgc agtccgcgcc tgcgaagagg
    14521 cgtcgcacca cgacgcgcct gctcgcgcga cgcgaagcac gcatcgcgtt cctcttcgtg
    14581 ctgccggcgt tcctgctctt catcgccttc cgcttcggcc cggccatcgc cggcgtcgcg
    14641 ctgagcttct tcgactacga catctccggc gagatcgact ggcgggggct cgacaacttc
    14701 cagcgcctct tcgaggacgc cctgttctgg cgggccctgg gcaccacgct gctctacacg
    14761 gtgttcgcgg tgccgatctc gctcgcgctc tccaccgtga tggcgctggg cgtgcggcgc
    14821 tcgttccgcg gctcgcggtt cttccgctcg atcttctttc tgccggtcat cacctcgctg
    14881 gtgctcgccg gtgcgatctt catctggatc ttctcccgca acggaccatg gtcgacgctc
    14941 atgtcgccgc tgggcctcgg cggctcctgg ctggcgagca ccgtgctggt ggtccccgcg
    15001 atcatcatcg tcggcgtctg gtcccgtttc ggctacggga tgatgatcat gatcgccgcc
    15061 atgcaggaca tcccccgcga gctcgaggag gcggcgctga tggacggcgc caacgcgtgg
    15121 cagcggttcc gctgggtcat cctgccgttc ctgcggccca ccgtgttctt cctcgccgtc
    15181 atcgagacga ccgccgcctt ccaggtgttc gacgtcatct acgtcatgac ccagggcggg
    15241 cctgcgaatg cgagctactc gctcgtgtac ctgatctacg accagggctt caagtacttc
    15301 gagtacggct atgcggcggc ggccggtgtc gccctgttcg tcatgaccct cgcggtcgcc
    15361 ctcatccagc gcctcgtgat cggaaagcag aaatgaccgc ggtgacgacg aacgcgcagc
    15421 tcaggcagcg ctcctacaag gcgctcgagc cgacgcgcgg cggcctggtg ctgcggtggg
    15481 tctggctcag catcgcggcc gtgttcagct tcttcccctt ctacgcgatg gtcgtgctga
    15541 gcctcaagcc cggcgcggtc gtgaacttcc cgcagtcgct gctgccgtgg ggcgacctgt
    15601 ccctctccgc gtacgagcag gtgctcagcg gccagaacat cctgctctgg ctgttcaaca
    15661 ccctggtcta cgccctcgtc tcggttgtcg cggtgctgtt cctctcggcg ctggccggct
    15721 acgccttcgc caagaagcgc ttcgcgggga aggaggtcat gttctggtcg ttcctgtcga
    15781 tggtcatggt ccccttccac gtgaccctga tccccacgtt cctgctgatc gccaacctgg
    15841 gcggcgtgaa cgagtactgg ggcctgatcc tgccgacgct ggcgaacgcc caggcggtgt
    15901 tcctcatgcg ccagttcatc gaggggctgc ccgacgagct gttcgaggcc gcgcgcatcg
    15961 acggcgccag cgagctgcgc atcttcctcc ggatcgtgct gccgctgtgc aagccggtgc
    16021 tggcgaccct cgggatcttc gtcttcctgt ggcactggaa cgacttcctg tggccgctga
    16081 tcatcgcgaa gtccaacgag atgttcacgc tgaccgtggg catctcctca ctgcagcagc
    16141 agaacgtgcc gctgagcgtg atgctcgccg gctcggtcgt cgcgctgctg ccgatcttca
    16201 tggcgtacct catcgcgcag cgatacgtgc aagaaggcgt gaccggctcg ggaatcaagg
    16261 gctgaaagga atgagtgacg tgacaacgaa gagcttcacc tcggaggctg agctggagca
    16321 ggcgctgacg gcgcccagcg atgcgctgat cgaggacttc tcgaggacct caggggatct
    16381 ggtcatcctc ggtgcgggcg gcaagatggg cccgaccctc gcggtgctcg cccggcgggc
    16441 gatggatgcc gcgggccgga acgccgacac ggtgtacgcc gtgtcgaggt tcggcgatcc
    16501 ggccgtcacc gagcggctgc ggagtgcgaa cgtggaggtc gtcccgttcg acctgatcga
    16561 gaacgacgac ttctcgtcgc tgcccgacgc cccgaatgtc gtgttcatgg tcggcgcgaa
    16621 gttcggtgcc gccaccagcc cgtcctgggc ctgggaggtc aacgcggcac tgcccgatcg
    16681 cgtcgcgcga cgctaccgcg acagcgcgat ctccgtgctg tccaccggca acgtctatcc
    16741 cttcctcccg gcctcctcgg gcggggcatc cgaagaggtg acgcccgcgc cgatcggcga
    16801 gtacgcgcag tcctgcctcg gccgcgagcg ggtgttcgag ttcggcgcgc agcagcgcgg
    16861 cacgaaggtg gcgctgatcc ggctgaacta cgcggtggac ctcaggtacg gggtgctcgc
    16921 cgacatcggc agcgccgtgc acgccggcga gccggtctcg ctggccaccg cgaacgtcaa
    16981 cgtgatctgg cagggctacg cgaacgaggt cgtgctgcgc agcctgctgc acgcgacatc
    17041 cgacgtgttc accatcaacc tgaccggccc ggagctgctg agcgtcgagt cgatcgcccg
    17101 ccgcttcggc gccctgttcg acagcgaagt gcacctcgtc gatgagccgc agccgaccgc
    17161 tctgctcagc gatgcgcggc gctgcatggg gctgttcggc tacccgtcgg tgcccgcgga
    17221 gacgctgatc gaactgcagg ccgcctggat cagggacgag ctgccgatga cggccaagcc
    17281 gaccaagtgg gccgtgcgag acgggaagtt ctgatgacca ccgttcccac cctgcgcgcc
    17341 gaagctgcgc agaccctctc ccgcggagcc gtcatccccg cgcatccgct cgccctgacg
    17401 gccgaccgcc ggctcgacga gcgtcgccag cgcgcgctga gccgctacta cctcgccgcc
    17461 ggtgccggcg gc
//
LOCUS       DATMSX010000059         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_48132,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000059 DATMSX010000000
VERSION     DATMSX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48132"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VN041_01765"
     CDS             <1..390
                     /locus_tag="VN041_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU27805.1"
                     /translation="FPRVSVLSLHAPALPSTHHMISVRELAALPDGATVINTARGALL
                     DHDALVAECRTGRLDAVLDVTDPEPLPLDSPLLDLPNVALTPHLAGSLGTETRRLTDS
                     ALDELQAYASGVPLLYPMDAVSLARSA"
     gene            complement(416..1096)
                     /locus_tag="VN041_01770"
     CDS             complement(416..1096)
                     /locus_tag="VN041_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HWU27806.1"
                     /translation="MKRVAHDTWASILGVLYLGLMVNLLVLVAASPLVVLLITTDPMR
                     SWPLLAVAAVPAVPALTAAFGAYRAHDEGETQVVRAYLRSWRATWRKAMLLGALAAGV
                     AVVLLVDVRMLADSAASVVIVPVLLLLAALTASTALLGAVALAEVPTARLRDVLRSSL
                     YLGVRRWYFQLLTFAVLGVQIAVFTTMPAIAIGLTAAPALYVAWANARYVLRPVLGEV
                     PARDEAAA"
     gene            complement(1100..2005)
                     /locus_tag="VN041_01775"
     CDS             complement(1100..2005)
                     /locus_tag="VN041_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU27807.1"
                     /translation="MSETQLMVTGEKRMRRLSEPAPIGSSQGARRVGRVFGYAALIVA
                     AIALLAPFAWMLLSSLKTSNEVFSAPIVWWPETFVWQNYLDIWSKSGMLTWIRNTLLL
                     AVVVTFLQVLTGSFAAYGFSRMRFPGRDVLFLAYIGTIAVPWQSYMIPQFILLSNLKV
                     SNTLWAIILIQAFGAFGVFLMKQYYETIPEELSEAARLDGLSEYGIWRRIMIPLSVPA
                     IASLTLLTFVNTWNDYLGPLIYLRNPDLWTIQLGLKSFVSNLFDTNYALLFAGLCISV
                     VPIAIIFMLGQKYFVEGIATSGMKG"
     gene            complement(2005..2925)
                     /locus_tag="VN041_01780"
     CDS             complement(2005..2925)
                     /locus_tag="VN041_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU27808.1"
                     /translation="MTSTVAPPPNRPPRRRSALRRRNTLIGWTFILPNFAGFAAFTLV
                     PVLILFYMAFTNWNVFGKADWVGFANFQRLLGDGSFRISFWNTIYYSVMHIPLTIVVS
                     LGLALLLNSKLRGVAFFRTAAFFPYITSIVAIAVVWNLLFSPDFGPINAVLRSIGIAH
                     PPGWLTSKDWALPAVVIVSTWRDMGYYMILFLAGLQTVPRELHEAARVDGANVWQRFL
                     NVTLPCLRPTMFFVTVMLTINSLKIFDLILVMTNGGPGQATMVLSQFIYRKGFVESQF
                     GYASAASVALFLLCIIVTIAQFLWNKRRSV"
BASE COUNT          549 a          999 c         1034 g          402 t
ORIGIN      
        1 ttcccccgcg tgagcgtgct ctcgctgcac gccccggcgc tgccctccac gcaccacatg
       61 atctcggtgc gggagctcgc cgcgctgccc gacggcgcca cggtcatcaa caccgcgcgg
      121 ggcgcgctgc tcgaccatga tgcgctggtc gcggagtgcc gcacgggccg gctggatgcc
      181 gtgctcgacg tcaccgaccc agagccgctg ccgctggact cgccgctgct ggatctgccg
      241 aacgtggcgc tcaccccgca cctggccgga tcgctcggca ccgagacccg gcggctgacg
      301 gactctgccc tggacgagct ccaggcgtac gcctccggcg tcccgctgct gtacccgatg
      361 gacgcggtct cgctggcgcg cagcgcctga gaggccgcgc ccgacggcga ggagatcacg
      421 ctgccgcttc gtcccgagcg ggcacctcgc cgagcacggg ccgcagcacg tagcgcgcgt
      481 tcgcccaggc gacgtagagc gccggcgccg cggtcaggcc gatcgcgatc gcgggcatgg
      541 tggtgaacac cgcgatctgc acgccgagca ccgcgaacgt cagcagctgg aagtaccagc
      601 gccgcacgcc cagatacagg ctcgagcgca gcacgtcccg cagccgggcc gtcggcacct
      661 cggcgagcgc cacggctccg agcagggcgg tcgaggccgt cagcgccgcc aggagcagca
      721 gcacaggcac gatcacgacc gaagcggccg aatccgcgag catccgcacg tcgaccagca
      781 gcaccactgc gactccggca gccagcgccc ccagcagcat cgccttccgc caggtcgcac
      841 gccaggagcg cagatatgcc cgcaccacct gggtctctcc ctcgtcatgc gcccggtacg
      901 cgccgaacgc ggcggtcagg gccggcacgg cgggcaccgc cgcgaccgcc agcaggggcc
      961 aggagcgcat cgggtcggtg gtgatcagca gcaccaccag cggggatgcc gccaccagca
     1021 cgagcaggtt caccatcagc cccaggtaca acacgcccag gatcgaggcc caggtgtcgt
     1081 gcgcgacgcg cttcacggct cagcccttca tcccgctggt cgcgatgccc tcgacgaagt
     1141 acttctgccc gagcatgaag atgatcgcga tcggcacgac cgagatgcac aggcccgcga
     1201 agagcagggc gtagttcgtg tcgaacaggt tggagacgaa gctcttcagg cccagctgga
     1261 tcgtccacag gtcggggttg cgcaggtaga tcagcggccc gaggtagtcg ttccaggtgt
     1321 tcacgaaggt cagcagcgtc aggctcgcga tcgcgggcac cgacaacggg atcatgatgc
     1381 gccgccagat gccgtactcg ctcaggccgt ccagccgtgc ggcctcgctg agctcctccg
     1441 ggatggtctc gtagtactgc ttcatcagga acacgccgaa cgcgccgaac gcctggatga
     1501 ggatgatcgc ccagagcgtg ttggacacct tgaggttgga cagcaggatg aactgcggga
     1561 tcatgtacga ctgccacggc acggcgatcg tgccgatgta cgccaggaac agcacatcgc
     1621 gtcccgggaa ccgcatgcgc gagaagccgt aggccgcgaa cgagccggtc agcacctgca
     1681 ggaaggtcac cacgaccgcc agcagcagcg tgttgcggat ccaggtcagc atcccggact
     1741 tcgaccagat gtcgaggtag ttctgccaga cgaacgtctc gggccaccac acgatcggcg
     1801 cactgaacac ctcgttcgag gtcttcagcg agctcagcag catccaggcg aacggcgcga
     1861 gcagggcgat cgcggcgacg atcagggccg cgtagccgaa cacgcggccg acgcggcgcg
     1921 caccctggga ggacccgatc ggggcgggct ccgagagccg gcgcatccgc ttctccccgg
     1981 tgaccatgag ctgggtctcg gacatcagac gctcctcctc ttgttccaga ggaactgggc
     2041 gatggtgacg atgatgcaca gcaggaacag tgcgaccgag gcggcggagg cgtagccgaa
     2101 ctgcgactcc acgaaaccct tgcgatagat gaactgcgag agcaccatgg tcgcctgccc
     2161 cggaccgccg ttcgtcatga cgaggatcag gtcgaagatc ttcagcgagt tgatggtgag
     2221 catcacggtg acgaagaaca tcgtcggccg caggcacggc agggtcacgt tcaggaagcg
     2281 ctgccagacg ttcgcgccgt cgacgcgcgc tgcctcgtgc agctcacgcg gcacggtctg
     2341 cagcccggcg aggaacagga tcatgtagta gcccatgtcg cgccaggtgc tgacgatgac
     2401 gacggcgggc agcgcccagt ccttcgaggt gagccagccg ggcgggtgcg cgatgccgat
     2461 ggagcgcagc accgcgttga tcggaccgaa gtcggggctg aacagcaggt tccacaccac
     2521 ggcgatcgcg acgatcgagg tgatgtacgg gaagaaggcg gcggtgcgga agaacgccac
     2581 cccgcgcagc ttgctgttca gcagcagcgc gagcccgagc gagacgacga tggtgagcgg
     2641 gatgtgcatc accgagtagt agatggtgtt ccagaacgag atccggaagc tgccgtcgcc
     2701 gagcagccgc tggaagttgg cgaacccgac ccagtcggcc ttgccgaaga cgttccagtt
     2761 cgtgaacgcc atgtagaaga ggatcagcac gggcacgagc gtgaaggccg cgaatccggc
     2821 gaagttcggc aggatgaacg tccacccgat cagggtgttc cggcggcgca gcgccgagcg
     2881 ccggcgcggc ggccggttcg ggggcggggc gacggtgctg gtcatggggt ttccttcgtc
     2941 gggctgaaga cggatggcgg acgcggggcg acggcggatg ccgg
//
LOCUS       DATMSX010000060         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_10241,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000060 DATMSX010000000
VERSION     DATMSX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10241"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VN041_01785"
     CDS             complement(<1..129)
                     /locus_tag="VN041_01785"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWU27809.1"
                     /translation="MTRTFPFRQAAIDLGAIAENVRHLRGLTGVQVIAVVKANGYGH"
     gene            complement(126..488)
                     /locus_tag="VN041_01790"
     CDS             complement(126..488)
                     /locus_tag="VN041_01790"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWU27810.1"
                     /translation="MIIGTGIDLVDVARFERSVTRTPRLLDRLFTPSEQQLRLRSLAA
                     RYAAKEALIKALGGSDGVYWTEIEIASEASGRPHFVLSGSTAAVVAERGITALHLSLT
                     HDAGLAAAYVIAERTDAA"
     gene            complement(665..2515)
                     /gene="glmS"
                     /locus_tag="VN041_01795"
     CDS             complement(665..2515)
                     /gene="glmS"
                     /locus_tag="VN041_01795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWU27811.1"
                     /translation="MCGIVGYVGPRPSQDILLAGLARLEYRGYDSAGIAVIDESGALG
                     MRKKAGKLKVLRDSLADAALADGTTGIGHTRWATHGGPTDGNAHPHLADDGKLALIHN
                     GIIENFATLRDELTAEGVTFLSETDTEVAAALVGREYRRTGDLASAFRNVVNRLEGAF
                     TLLAVHQDEPGLVVGARRNSPLVIGLGEGENFLGSDVAAFVEHTRNALAIGQDQIVAI
                     TPDSVTVTDFDGNSVEAQPFEVSWDAAAAEKGGWSSFMAKEVAEQPEAVANTIRGRIQ
                     GDEVVIPELDGLDDLFTGINRVIITACGTASYAALVGKYAIEQWARVPVDVELAHEFR
                     YRDPVIGDDTLVVSISQSGETMDTLMAVKYANERGAKTLSICNTQGATIPRESDAVVY
                     THAGPEVAVASTKAFSAQITALLLLGLHMGRVRGTASVSDVRDAVGELQSLPEKVAQV
                     LAEETEHVSQLAGWMADTRSVLFLGRHVGFPIALEGALKLKEISYIHAEGFAAGELKH
                     GPIALIEPGQPVFVLVPSPRHSALIHSKVVSNIQEIRARGARVIVVAEEGDAAVLPYA
                     DEVIRIPLAGPLFEPILAVVPLQIFAMALATAKGLDVDQPRNLAKSVTVE"
     gene            complement(2574..3515)
                     /gene="coaA"
                     /locus_tag="VN041_01800"
     CDS             complement(2574..3515)
                     /gene="coaA"
                     /locus_tag="VN041_01800"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HWU27812.1"
                     /translation="MTAATNLPLSQYREIDRADWARLSAGLDQPLSETEVVGIRGIGD
                     RLNLDEVREVYMPLSRLLSLYASSIRKLGAATSEFLQEDDSTTPFVVGVAGSVAVGKS
                     TIARLLRELMSRWPGTPRVELVTTDGFLYPNAELERRGLMDRKGFPESYDRRALISFL
                     TEVKSGAAEVRAPFYSHMRYDIVPDAHVVVRSPDVVIVEGLNVLQPPPAPNDVAVSDL
                     FDFSIYVDADSKHIERWYIDRFLALRKGAFANPSSYFNVFAHLTDDEAVTTALGYWNE
                     INMPNLLENVMPTKHRATLVLRKGADHAVESVQLRKV"
     gene            3605..4555
                     /locus_tag="VN041_01805"
     CDS             3605..4555
                     /locus_tag="VN041_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HWU27813.1"
                     /translation="MRLGVLDIGSNTVHMLAADIHPGGRPLATTSDRTVLRLMRYLTP
                     EGAISPEGVQALEDAVDQARRVADADDLHTLIATATSAVREAANGAEVITRIEARLGQ
                     PLQVLTGEMEAELTFLAVRRWFGWSAGRLMLLDIGGGSFEFAAGEDELPEVSASAPLG
                     AGRMTLRFLSDDPPGEERVAVLRAHAESTLAPIVESFRVLPDPDHVVGTSKAIRSLAR
                     LVGYPTRGWSGSERMLLPRASLASWVPRLARIPASARQELPGITPDRTFQITAAAVCL
                     LTAMEMLDIDELEVCPWALREGVLLRYIEQSGMESTLPLR"
     gene            4810..5463
                     /locus_tag="VN041_01810"
     CDS             4810..5463
                     /locus_tag="VN041_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HWU27814.1"
                     /translation="MTAGSEFSLAALFASTHEDVPDVQPNIMTVCTGNICRSPLAETV
                     LATRLADLDLRVHSAGTQALVGYGMPNEARTLAERNGVSPERTAAHRARLLSETLMND
                     ADLILTMTAQHSTAAMQLTPRRMHSTFTVREFARLAASLDDGVLRRIARSAGNPRSRF
                     GVLVQAVADQRGVAPRAVGDEDVIDPYRQSLEVYEQSAAQLLPALAQVERVVRIALA"
     gene            complement(5477..6376)
                     /locus_tag="VN041_01815"
     CDS             complement(5477..6376)
                     /locus_tag="VN041_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27815.1"
                     /translation="MATVMNFALGEANELAHAMMARIGRDHGIRTLSIKGLVADRYGL
                     RRPRVAADADVIIDPARFDEFCRLLRDYGWHLRVEREVPSLLGPHSVTLIRDDWPNDI
                     DAHVRFPGFFADDQTVFERLWSTRSTMEVAHCEVTVPSRAASAVIAALHAVRYTKSVR
                     HAGELERVAELLIDDFSDAEREEFLDVACVGRAQWVLRELFDLVGRPYEMDAAPRQMQ
                     LWETNRATVEDGAAVSWLSTLRSVPLHRKPAVLARALWISREEIPRNDVNRLPTMREA
                     WAHRMLRWRRGGIALWHYARWRR"
     gene            complement(6430..6717)
                     /locus_tag="VN041_01820"
     CDS             complement(6430..6717)
                     /locus_tag="VN041_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HWU27816.1"
                     /translation="MTGWGSASVRIGDDVAWTADESRVVALDLAQPGASPLVLESTSA
                     VIWEEIAIRGPIQVDELLGLLAQAFDVQVDEIRPDVDSLLAELRSRRLLAD"
     gene            6792..7664
                     /locus_tag="VN041_01825"
     CDS             6792..7664
                     /locus_tag="VN041_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU27817.1"
                     /translation="MASSSVTSLISAHLGIPTHVAGLVAARSFRSITMTWHRARETAW
                     RWSPIEVGTRRSTVVLFPGDGRKVPTADAAPSGPAPLGLFLPPDAEQSLEWEPGSDVL
                     AVWVPERLLQEFTEGRTPDAAVLHSSPLTIGFRTFAQAVVRHGDEGSSMSQYAIERLV
                     AEMLFGALVEQQSIETSPRPSSLVERARSIMLMHREDPNFSTPQLAAELHISPRQLQR
                     AFAGAGLRPGDMLRRMRIELAESMLRNPLYTGLTVEEISRYAGFTSALQLRRALHAEG
                     SPAPVELRNAERGA"
BASE COUNT         1170 a         2670 c         2694 g         1168 t
ORIGIN      
        1 gtgtccgtag ccgttggcct tgaccacggc gatcacctgc acgccggtga ggccgcgcag
       61 gtggcggacg ttctcggcga tcgcgccgag gtcgatcgcg gcctgacgga acgggaaggt
      121 gcgggtcatg cggcatccgt cctctcggcg atgacgtacg cggcggcgag gccggcgtcg
      181 tgggtgaggc tcaggtgcag ggcggtgatg ccgcgctcgg cgacgaccgc cgcagtggat
      241 ccgctgagca cgaagtgggg gcggccggag gcctcggatg cgatctcgat ctcggtccag
      301 tacacgccgt cgctgccgcc gagcgccttg atcagcgcct ccttggcggc gtaccgcgcg
      361 gccagtgagc gcaggcgcag ctgctgctcc gaaggggtga agagacgatc caggaggcgc
      421 ggagtgcggg tcaccgagcg ctcgaaccgc gcgacgtcga cgaggtcgat tccggtgccg
      481 atgatcaccc tttcagccta ctcggggcgt cggatgcccg gcatccgggg ccccgtcgac
      541 gcgagcggga caagggcaga cagagatcgg ggccggcacg ccacttctcg cacgtctcca
      601 gagagaacgt ccgagatcgg gcgtgccggc ccccgcaatc cgacgagcga gccgagccgt
      661 cagctcactc cacggtgacc gacttcgcga ggttgcgggg ctggtcgacg tccaggccct
      721 tggccgtggc gagcgccatg gcgaagatct gcagcgggac gaccgcgagg atcggctcga
      781 acagcggccc ggcgagcggg atgcggatga cctcgtcggc gtagggcagc acggcggcgt
      841 caccctcctc cgcgacgacg atcacacgag caccgcgggc gcggatctcc tggatgttcg
      901 agacgacctt ggagtggatc agtgcggaat ggcgcggcga gggcaccagc acgaacacgg
      961 gctgaccggg ctcgatcagc gcgatcgggc cgtgcttgag ctcgcctgcg gcgaatccct
     1021 cggcgtggat gtaggagatc tccttgagct tgagcgcgcc ctcgagcgcg atcgggaacc
     1081 cgacatgacg acccaggaac agcaccgagc gggtgtcggc catccagccg gccagctgcg
     1141 agacgtgctc ggtctcctcg gccagcacct gcgcgacctt ctcaggcagc gactgcagct
     1201 cgccgacggc atcacggacg tcggataccg aggccgtgcc gcgcacccgc cccatgtgca
     1261 ggcccagaag cagcagcgcc gtgatctgcg cggagaacgc cttggtcgac gcgacggcga
     1321 cctcggggcc ggcatgcgtg tagacgacgg catccgactc gcgcgggatg gtcgcgccct
     1381 gtgtgttgca gatcgacagg gtcttcgcgc cgcgctcgtt cgcgtacttg accgccatca
     1441 gggtgtccat cgtctcgccg gactggctga tcgagacgac gagggtgtcg tcgccgatca
     1501 ccgggtcgcg gtagcggaac tcgtgcgcga gctcgacgtc aacgggcacg cgcgcccact
     1561 gctcgatcgc gtacttgccg accagtgccg catacgacgc ggtgccgcag gcggtgatga
     1621 tcacgcggtt gatgccggtg aacaggtcgt cgagtccgtc gagctcgggg atgaccacct
     1681 cgtcaccctg gatccggccg cggatggtgt tcgcgacggc ctcgggctgc tcggcgacct
     1741 ccttggccat gaacgaggac cagccgccct tctcggcggc cgcggcgtcc caggacacct
     1801 cgaagggctg agcctcgacg gagttgccgt cgaagtcggt gaccgtcacc gagtcggggg
     1861 tgatcgcgac gatctggtcc tgcccgatgg cgagcgcgtt gcgggtgtgc tcgacgaacg
     1921 cggcgacatc cgagccgagg aagttctcac cctcgcccag gccgatcacc agcggcgagt
     1981 tgcgacgggc gccgacgacc agacccggct cgtcctggtg gacggccagc agcgtgaacg
     2041 caccctccag gcggttgacc acgttgcgga aggcggaggc caggtcgccg gtgcggcggt
     2101 actcccggcc gaccagggcg gccgcgacct cggtgtcggt ctcgctgagg aacgtgacgc
     2161 cctcggcggt cagctcgtca cgcagcgtgg cgaagttctc gatgatgccg ttgtggatca
     2221 gcgccagctt gccgtcgtcg gccaggtgcg ggtgcgcgtt gccgtcggtc gggccgccat
     2281 gcgtcgccca gcgggtgtgg ccgataccgg tcgtgccgtc ggcgagcgct gcatccgcca
     2341 gcgagtcgcg cagcaccttg agcttgcctg ccttcttgcg catgccgaga gcgccgctct
     2401 cgtcgatgac ggcgatgccc gccgagtcgt agccgcggta ttcgagacgg gcgagacccg
     2461 cgagaaggat gtcctggctg ggccgcgggc ccacgtatcc gacgattcca cacatgggat
     2521 caaggataag cgggggtttt tgcgaggcct ctgaacgaac gtcggagccg cggtcacacc
     2581 ttgcgcagct gcacgctctc gaccgcatga tcggcaccct tgcgcagcac gagcgtcgcg
     2641 cggtgcttgg tgggcatgac gttctcgaga aggttcggca tgttgatctc gttccagtac
     2701 ccgagagccg tggtcaccgc ctcgtcgtcg gtgaggtgcg cgaacacgtt gaagtacgac
     2761 gacgggttcg cgaacgcacc cttgcgcagc gcgaggaagc gatcgatgta ccagcgctcg
     2821 atgtgcttgg agtcggcgtc gacgtagatc gagaagtcga acaggtcgct gacggccaca
     2881 tcgttcggag cgggcggcgg ctgcagcacg ttcagcccct ccacgatcac cacgtcgggg
     2941 ctgcgcacga cgacgtgcgc gtcgggcacg atgtcgtacc gcatgtgaga gtagaacggc
     3001 gcgcgcacct cggccgcccc gctcttcacc tcggtgagaa acgagatcag cgcacgacgg
     3061 tcgtaggact cggggaagcc cttgcggtcc atcagcccgc gacgctcgag ttctgcgttc
     3121 ggatacagga agccgtcagt ggtgacgagc tcgacccgcg gcgtccccgg ccagcggctc
     3181 atgagctcgc gcagcagccg cgcgatggtc gatttgccga ccgccacgga gccggcgacg
     3241 cccaccacga acggcgtggt ggagtcgtcc tcctgcagga actcgctggt cgcggcgccg
     3301 agcttgcgga tcgacgacgc gtacaggctg agcaggcggc tcagcggcat gtagacctcg
     3361 cgaacctcgt cgaggttcag ccggtcgccg atgccgcgga tgccgacgac ctcggtctcg
     3421 ctgagcggct gatcgagacc tgccgacagg cgcgcccaat cggcgcggtc gatctcgcgg
     3481 tactgggaca gcggcaggtt ggtggcggcg gtcacgggtt acatcgtatc cgtcgggtga
     3541 acagcggatg tcgcggcgat cggagaggga tgtcgcggcg ccctcgcacg agtaatctcg
     3601 tgccgtgcgc ttgggagtcc tcgacatcgg ttccaacacc gtccacatgc tggcggccga
     3661 catccacccc ggtggacgcc cgctggcgac gacgagcgac cggacggtgc tgcgcctcat
     3721 gcgctacctc acacccgagg gcgcgatcag ccccgaggga gtgcaggctc tggaggatgc
     3781 cgtcgatcag gctcgtcggg tcgccgacgc cgacgacctg cacacgctga tcgccacggc
     3841 caccagcgcg gtgcgcgagg cagccaacgg cgcggaggtc atcacccgca tcgaggcgcg
     3901 gctcggtcag ccgctgcagg tgctgaccgg agagatggag gccgaactca cgttcctcgc
     3961 cgtgcggcgc tggttcggct ggtcggcggg gcggctcatg ctgctcgaca tcggcggcgg
     4021 ctcgttcgag ttcgccgccg gcgaggacga actgcccgag gtctcggcat ccgctcccct
     4081 cggcgccggc cgcatgacgc tgcggttcct cagcgacgac ccacccggtg aggagcgcgt
     4141 cgccgtgctg cgggcgcacg ccgagagcac tttggcgccg atcgtcgaga gcttccgcgt
     4201 gctccccgat cccgaccacg tcgtcggcac gtcgaaggcg atccggtcac tggcacggct
     4261 ggtcggctat ccgacgcgcg gctggtccgg gtcggagcgg atgctgctcc cgcgcgcgtc
     4321 gctggcatcc tgggtgccgc gcctggcccg catcccggca tccgctcggc aggagctccc
     4381 cgggatcacg ccggaccgca cgttccagat cacggcggcg gcggtgtgcc tgctcaccgc
     4441 gatggagatg ctggacatcg acgaactcga ggtgtgtccg tgggcgctgc gcgagggggt
     4501 gctgctgcgg tacatcgagc agtccggaat ggagtcgacg ctcccgctgc gctgacccgt
     4561 cctcgctcga cgcgctcgcc acccgccgcg tcggatcccg gaatcgatgg tggctgggaa
     4621 agcagccgcg gctagagtgt gaggatgcat cgatctgagg atcgcggcct tctggggagg
     4681 ccaggcagcg gaatcgacgc acccatggcg aggactcgcc gtgagcgtcg cctgctgcgt
     4741 gcggaccccg aaacgccgct gccggccctc gactccgacg ggcggtcgcc gagaccccag
     4801 gagacgacca tgaccgctgg atccgagttc tccctggccg cactgttcgc gagcacccac
     4861 gaggacgtcc ccgacgtgca gccgaacatc atgacggtgt gcaccggcaa catctgccgc
     4921 tcgcccctgg ccgagaccgt gctggccacg cgactcgccg acctcgatct ccgcgtgcac
     4981 agcgcgggga ctcaggcact cgtcgggtac gggatgccga acgaggcgcg cacgctcgcc
     5041 gagcgaaacg gcgtctcacc ggagcgcacc gccgcgcatc gcgcgcgcct gctgtccgag
     5101 acgctgatga acgacgcgga tctgatcctg accatgacgg ctcagcacag caccgccgcc
     5161 atgcaactca cccctcgtcg catgcacagc acgttcacgg tgcgcgagtt cgcgcgtctg
     5221 gccgcctcgc tcgacgacgg cgtgctgcgg cgcatcgcac gcagcgcggg caacccgcgg
     5281 tcccgtttcg gcgtgctggt gcaggccgtc gccgatcagc gcggcgtcgc gccgcgggcc
     5341 gtcggcgacg aggacgtgat cgacccgtac cgacagtccc tcgaggtgta cgagcagtcc
     5401 gcagcgcagt tgcttcccgc cctggcccag gtggagcggg tcgtccgcat cgccctggcc
     5461 tgacacccag gatcggtcag cggcgccagc gggcgtagtg ccacagggcg atgccgccgc
     5521 gcctccagcg cagcatccgg tgcgcccacg cctcgcgcat cgtcggcagt cgattcacgt
     5581 cgttgcgcgg gatctcctcg cgtgagatcc acagagcgcg cgcgaggacg gccggcttgc
     5641 ggtgcagcgg caccgagcgc agcgtgctca gccaggagac ggccgcgccg tcctccacgg
     5701 tcgctcggtt ggtctcccat agctgcatct ggcgcggcgc ggcatccatc tcgtaggggc
     5761 gcccgacgag gtcgaacagc tcacgcagca cccactgcgc ccttccgacg catgcgacgt
     5821 cgaggaactc ctcgcgctcg gcatccgaga agtcatcgat gagcagctcc gcgacgcgct
     5881 ccagctcgcc ggcgtggcgc acggacttgg tgtagcgcac ggcgtgcagt gcggcgatga
     5941 cggcgctcgc ggcccgcgag ggcaccgtca cctcgcagtg cgccacctcc atggtcgagc
     6001 gcgtgctcca cagccgctcg aagacggtct ggtcgtcggc gaagaatccc gggaaccgca
     6061 catgcgcgtc gatgtcgttc ggccagtcgt cgcggatgag cgtgaccgaa tgcggtccga
     6121 gcagcgaggg cacctcgcgc tccacgcgca gatgccagcc gtagtcgcgc agcagccggc
     6181 agaactcgtc gaagcgggcg gggtcgatga tcacgtcggc atcggcggcg acccgtggcc
     6241 gacgcaggcc gtagcgatcg gccaccagcc ccttgatcga cagggtgcgg atgccgtggt
     6301 cgcggccgat ccgcgccatc atggcgtgcg cgagctcgtt cgcctcgccc agcgcgaaat
     6361 tcatgacagt cgccacgtca ccccccttct ctctccgacc ataccgacgc agcgcgccgc
     6421 gcgttcgact cagtcggcca gcagccgacg tgatctcagt tcggcgagca ggctgtcgac
     6481 gtcggggcgg atctcgtcga cctgcacatc gaacgcctga gcgagcagtc ccaggagctc
     6541 gtccacctgg atcggaccgc ggatcgcgat ctcctcccag atgacggccg aggtggattc
     6601 cagcaccaga gggcttgcgc cgggctgcgc gaggtcgagt gcgacgacgc gagactcgtc
     6661 cgcggtccag gccacatcgt cgccgatgcg caccgatgcg ctcccccacc ccgtcacgct
     6721 tggtagccta gcgcctgtgc gcgtcacacc cccggtggcg tgcatggcaa ccggactgca
     6781 tcctcccccc aatggcatcc tccagcgtca cctcgttgat ctctgcgcac ctgggcattc
     6841 ccacccacgt cgcagggctg gtcgccgcgc gctcgttccg gtcgatcacc atgacctggc
     6901 atcgggccag ggagacggcc tggcgctggt cgccgatcga ggtgggaacg cggcgcagca
     6961 cggtggtgct cttccctgga gacggccgca aggtgccgac cgcggatgct gctccctccg
     7021 gtcctgcgcc cctcggcctg ttccttccgc ccgacgccga gcagagcctg gagtgggagc
     7081 cgggctctga tgtgctggcg gtgtgggtgc cggagcgact gctgcaggag ttcaccgagg
     7141 gcaggacccc ggatgccgcc gtgctgcact cctcgccgct cacgatcggc ttccgcacct
     7201 tcgcacaggc cgtcgtgcgc cacggggacg agggatcgtc catgtcgcag tacgcgatcg
     7261 agcgcctcgt cgccgagatg ctgttcggcg cgctggtgga gcagcagtcg atcgagacga
     7321 gccctcgccc cagctcgctc gtggaacgcg cccgctcgat catgctgatg caccgtgagg
     7381 acccgaactt ctccacccct cagctcgccg ccgagctgca catctcccca cggcagctgc
     7441 agcgcgcctt cgcgggagcc ggtctccgac cgggagacat gctgcgccgg atgcgcatcg
     7501 aactcgcgga gtcgatgctg cgcaacccgc tgtacacggg cctgacggtg gaggagatct
     7561 cacgctatgc gggcttcacg tccgccctgc agctgcgtcg ggcgctgcac gccgagggtt
     7621 cccccgcacc cgtcgagcta cgcaacgccg agcgcggcgc ctgagcgagc gcgggcgcct
     7681 gcccgccgtc gactgagcgg at
//
LOCUS       DATMSX010000061         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_95624,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000061 DATMSX010000000
VERSION     DATMSX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95624"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VN041_01830"
     CDS             <1..1018
                     /locus_tag="VN041_01830"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWU27818.1"
                     /translation="TALPDAALGDLVHAGPIALAAEDIGEGRAAYDAIDAADAEIVLV
                     LDHGHVVGTLTPRTALRSSLYGPAVDSSGRLAVAAAVGINGDVAGKARALASAGVDVL
                     VIDTAHGHQEGMLRALQTVAGLDLGIPLVAGNIVTADGVHDLVRAGASILKVGVGPGA
                     MCTTRMMTAVGRPQFSAVLETAQAARELGAHVWADGGVRYPRDVALALAAGAASVMIG
                     SWFAGTIEAPGELMHDAEGRAYKESWGMASTKAVQARFERLDPYELARKELFAEGISS
                     SMIYLDPLRPGVEDLLDMITSGVRSSFTYAGAATVAEFHDRALVGLQSASGYEEGKAL
                     PVSW"
     gene            1134..2459
                     /locus_tag="VN041_01835"
     CDS             1134..2459
                     /locus_tag="VN041_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWU27819.1"
                     /translation="MEFVLLGVGLLLIVGTGLFVASEFALVNLDRAELEARQARGESR
                     LMLTIRALKITSTHLSSAQLGITLTTLLTGYTMEPAISKLLEPLLEAWGVPDAAAVPV
                     SVTVAMLVATFLSMIIGELVPKNFALALPVQTAKLVIPFQTAFTVVFKPAVLMLNGSA
                     NAVLRAMGIEPKEELSGARSAEELSSLVRRSASQGVLEEDTATLLDRSLNFARLTADD
                     IMTPRPSMHAISVGDCADDVIRLARSTGHSRFPVYDDDLDDITGVVHVKAAVSVPREK
                     RLEVPVGALSTEPLRVPETVHLDALVAELRSKGYQMAIVVDEYGGTAGIVTLEDLIEE
                     IVGEVFDEHDRSRAGIVRRVDSITFPGELRPDELLARTGIEVPEGEVYDTIGGYMMSL
                     LERVPALGDEVRIGSGTLTVVRMDGRRVDRIRYIADPATETDHETEARR"
     gene            2456..3511
                     /locus_tag="VN041_01840"
     CDS             2456..3511
                     /locus_tag="VN041_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWU27820.1"
                     /translation="MSDWAGLVWLIVLLLANAFFVGGEFAVISARRSQIEPLAEQGSR
                     AAKTALYAMEHATLMLATSQLGITICSLLILNVSEPAIHHLLEIPMHAMGWDPALISV
                     LAFVITLALVSFLHVVFGEMVPKNLAFSVPDRAVLILAPPLVWVSRVFRHVIALLNGS
                     ANLVLRLFGVEPKNEAASTFTIEEVATIVDRSRREGVLTDTSGAVAAAVEFTDKKARD
                     VAVPLAELITLPDTATPGDVEKAVARYGFSRYVIVDDEDVAIGYVHLKDVLRASEESD
                     SHIDEPLPAKRIHHMVPVQEDTDLEDALAAMRRGGRHLAKVRDSSGETTAVLFLEDIL
                     EELVGEVQDATRRGHGR"
BASE COUNT          510 a         1249 c         1254 g          557 t
ORIGIN      
        1 gacggcgctg ccggacgccg ccctcggcga cctcgtgcac gccgggccga tcgcactggc
       61 cgccgaggac atcggcgagg gacgcgccgc gtacgacgcg atcgacgcgg cggacgccga
      121 gatcgtcctg gtgctcgatc acgggcacgt ggtcggcacc ctgaccccgc gcaccgctct
      181 tcgctcgagc ctgtacggtc ccgcggtgga ctcctcagga cgactcgctg tcgcggccgc
      241 ggtcggcatc aacggggacg tcgcgggcaa ggcccgcgcc ctggcatccg ccggagtcga
      301 cgtgctggtg atcgacacgg cccacgggca tcaggagggg atgctgcgcg cgctgcagac
      361 cgtcgcgggt ctggatctcg gcatcccgct ggtcgcgggc aacatcgtca ccgccgacgg
      421 cgtgcacgat ctcgtgcggg ctggggcgtc gatcctcaag gtcggcgtgg ggccgggagc
      481 catgtgcacg acgcgcatga tgaccgccgt cgggcgcccg cagttctccg ccgtgctcga
      541 gaccgcgcag gcggcgcgcg agctcggcgc gcacgtgtgg gccgacgggg gagtgcgcta
      601 cccgcgtgac gtcgcgctcg cgctcgccgc gggcgccgcg tccgtcatga tcggatcctg
      661 gttcgccggc acgatcgagg cgccgggcga gctgatgcac gacgccgagg ggcgcgcgta
      721 caaggagtcc tgggggatgg cgtcgacgaa ggcggtgcag gccagattcg agcggctgga
      781 cccctacgag ctggcccgca aggagctgtt cgcggagggc atctcctcgt cgatgatcta
      841 cctggacccg ctccggcccg gcgtggagga cctgctggac atgatcacct cgggcgtgcg
      901 ctcctcgttc acctatgcgg gggccgcgac cgtcgccgag ttccacgacc gggccctggt
      961 cggcctgcag tcggcctccg gctacgagga gggcaaggcg ctgccggtga gctggtagcg
     1021 tccgcgcggc acacagcagg attctgtaag atcgtcggca ccatggacga ccctccttct
     1081 tgcagtccct cgccccaccg cccgatcctc gccctgagtg aggacttcag ctgatggaat
     1141 tcgtgctgct gggcgtgggg ctcctcctca tcgtcgggac cggcctgttc gtggccagcg
     1201 agttcgccct ggtcaacctc gaccgcgcag agctcgaagc gcgccaggcg cgcggcgagt
     1261 cccggctgat gctcaccatc cgcgcgctca agatcacctc gacgcatctc tcctcggcgc
     1321 agctgggcat cacgctcacc acgctgctga ccggatacac catggagccg gcgatctcga
     1381 agctcctcga gccgctgctc gaggcatggg gcgtcccgga tgccgcggcc gtaccggtct
     1441 ccgtcaccgt ggcgatgctc gtcgccacat tcctgtcgat gatcatcggc gagctggtgc
     1501 cgaagaactt cgcgctcgcc ctcccggtgc agacggccaa gctggtcatc ccgttccaga
     1561 cggcgttcac cgtggtgttc aagcccgcgg tgctgatgct caacggcagc gcgaacgccg
     1621 tgctgcgcgc catgggcatc gagccgaagg aggagctctc cggcgcgcgc agcgccgagg
     1681 agctctcctc gctggtgcgc cggtcggcga gccagggcgt gctcgaggag gacacggcga
     1741 cgctgctgga ccgcagcctc aacttcgccc gcctcaccgc cgatgacatc atgacccccc
     1801 gcccgagcat gcacgccatc tcggtcggcg actgcgcgga cgacgtcatc cgcctggcgc
     1861 gcagcaccgg tcacagccgt ttccctgtgt acgacgacga cctcgacgac atcacgggcg
     1921 tcgtgcatgt gaaggccgcc gtgtccgtgc cccgcgagaa gcgcctggag gtgcccgtcg
     1981 gagcactcag cacggagccg ctgcgggtgc cggagaccgt gcacctcgac gccctggtcg
     2041 ccgagctgcg ctcgaagggg taccagatgg cgatcgtcgt cgacgagtac ggcggcaccg
     2101 ccggcatcgt cacgctggag gacctcatcg aggagatcgt cggcgaggtg ttcgacgagc
     2161 acgatcgctc ccgcgcgggc atcgtccgcc gggtcgactc gatcacgttc cccggtgagc
     2221 tgcgtccgga cgaactcctc gctcgcacgg gcatcgaggt gcccgagggt gaggtctacg
     2281 acaccatcgg cggctacatg atgagtctgc tcgagcgcgt ccccgccctc ggtgatgagg
     2341 tgcgcatcgg atccggcact ctcaccgtgg tgcgcatgga cggcaggcgg gtggatcgca
     2401 tccgctacat cgccgacccc gccaccgaga ccgaccacga gacggaggcc cgccgatgag
     2461 cgactgggca ggcctggtgt ggctgatcgt gctgctgctc gccaacgcgt tcttcgtcgg
     2521 cggcgagttc gccgtcatct ccgcgcgccg ctcgcagatc gagccgctcg cggagcaggg
     2581 ctcgcgcgcg gcgaagaccg cgctgtacgc catggagcac gccacgctga tgctggcgac
     2641 ctcgcagctg ggcatcacga tctgctcgct gctgatcctg aacgtgtccg agccggcgat
     2701 ccatcatctg ctggagatcc cgatgcacgc gatgggctgg gaccccgcgc tgatctcggt
     2761 gctcgccttc gtgatcaccc tggccctggt gtcgttcctg cacgtcgtgt tcggcgagat
     2821 ggtgccgaag aacctggcgt tctcggtgcc cgaccgcgcc gtgctgatcc tcgcaccccc
     2881 gctggtgtgg gtctcccgcg tgttccggca cgtgatcgcc ctgctgaacg gctcggcgaa
     2941 cctggtcctg cggctgttcg gcgtggagcc gaagaacgag gcggcctcga ccttcacgat
     3001 cgaggaggtc gcgacgatcg tcgaccggtc gcgtcgcgag ggcgtgctga cggacacgtc
     3061 cggcgcggtc gccgctgcgg tcgagttcac cgacaagaag gcgcgggatg tcgctgtgcc
     3121 gctggccgag ctgatcaccc tgcccgacac cgcgacgccc ggcgatgtgg agaaggccgt
     3181 ggcccgctac gggttctcgc gctacgtgat cgtcgacgac gaggacgtgg cgatcggcta
     3241 cgtgcacctc aaggacgtcc tgcgggcctc cgaggagtcc gactcgcaca tcgacgagcc
     3301 gttgcccgcc aagcggatcc accacatggt gcccgtgcag gaggacaccg acctggagga
     3361 cgcgctcgcc gcgatgcgcc ggggcggacg tcacctcgcg aaggtgcggg attcctcggg
     3421 ggagacgacc gcggtgctgt tcctggagga catcctcgag gagctcgtcg gcgaggtgca
     3481 ggatgccacg cgccgcgggc acggtcgctg agcgccgcgc catcgccgag tgcgacgcgg
     3541 ctgctgagcg ctgcctcccg cggaggtgac
//
LOCUS       DATMSX010000062         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124064,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000062 DATMSX010000000
VERSION     DATMSX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124064"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VN041_01845"
     CDS             <1..231
                     /locus_tag="VN041_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWU27821.1"
                     /translation="SARLLAIAHSLEELKTICRCGRKAVFNGRVINGRFVFDGDQVAI
                     DEGAATGAPADVVTYESLCGDCYLQESGGRLG"
     gene            295..999
                     /locus_tag="VN041_01850"
     CDS             295..999
                     /locus_tag="VN041_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU27822.1"
                     /translation="MTERAWETVLEHIERDLLDGRLGPGDRLPSERDLAADLGVGRSS
                     VREALRVLEVMGLIRTATGSGPQAGAIVIAAPSGGMSTLLRLQVAAQGFPLADVVQTR
                     LVLEDAVVARLSADPRRDLAGIRGILTAMDADDLTAPEFLALDAELHYALAEASGNTV
                     IAATMAGLRTAIESYVLEGSARIEDWDAMASRLRTEHHAIVDAIEAGGADAARALVHD
                     HITGYYTAAGLVPGDH"
     gene            1026..2285
                     /locus_tag="VN041_01855"
     CDS             1026..2285
                     /locus_tag="VN041_01855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWU27823.1"
                     /translation="MVQRQLPNPAELLELMKFKKPELDGRKRRLDAALTIYDLRTIAK
                     RRTPKAAFDYTDGAAEGELSLARARQAFEDVEFHPGILKPAPTVDTSVEILGGPSALP
                     FGIAPTGFTRLMQTEGETAGAGAAAAAGIPFTLSTLGTTSIEGVKAANPNGRNWFQLY
                     VMRDREISYELTRRAAAAGFDTLQFTVDTPVAGARLRDKRNGFSIPPQLTLGTIINAI
                     PRPWWWYDFLTTPKLEFASLSTTGGTVGELLDAAMDPTISYDDLAVIRDLWPGKIVIK
                     GVQNVEDSVRLKDAGVDGIVLSNHGGRQLDRAPIPFRLLPHVRKAVGDDFTVMVDTGI
                     MNGADIVASVALGADFTLIGRAYLYGLMAGGRQGVDRTIAILRTEIERTMRLLGVSTL
                     AELEPKHVTQLARLVPVAEGAAESVVR"
     gene            complement(2419..3192)
                     /locus_tag="VN041_01860"
     CDS             complement(2419..3192)
                     /locus_tag="VN041_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWU27824.1"
                     /translation="MPTPRLVAFDLDDTLAPSKGEIDVRIADLLRALLQRVDVAIISG
                     GNEDQFRTQVIARLADTMASDLARLHLLPTCGTRYLRHDGAAFAAVYAHDLTDQEKSD
                     ALTALREEAERLGLWESRTWGEILEDRGSQITFSALGQQAPRDAKHAWDPDGAKRALL
                     RDAVAARLPGLEVRSGGSTSIDITRAGIDKAYGMRQLADHTGIPLADMLFYGDRLDEG
                     GNDYPVLAIGVPSVAVEGWEDTADKLDELIAGLPDRVAR"
BASE COUNT          515 a         1223 c         1213 g          518 t
ORIGIN      
        1 tccgcccgcc tgctggccat cgcgcattct ctggaggagc tcaagacgat ctgccgctgc
       61 gggcgcaagg ccgtcttcaa cggccgcgtg atcaacggac gcttcgtgtt cgacggagac
      121 caggtggcga tcgacgaggg cgcggcgacc ggagcgccgg ccgatgtcgt cacctacgag
      181 tcgctgtgcg gcgattgcta cctgcaggag tccggcgggc gcctgggctg acccgccgtc
      241 gtttgtgtgg tcagaccaca cgatgtacgc tgtggtcaga ccacaggagg cgtcgtgacg
      301 gaacgagcat gggagacagt gctcgagcac atcgagcgtg atctgctgga cggccggctc
      361 ggcccgggcg atcggctccc ctcggagcgc gatctcgccg cggatctcgg agtggggcga
      421 tcgagcgtcc gcgaggcgct gcgcgtgctc gaggtgatgg ggctgatccg cacggcgacc
      481 ggctccggac cgcaggcggg cgcgatcgtc atcgccgcgc cctccggagg catgtcgacg
      541 ctgctgcggc tccaggtggc cgcacagggc ttcccgctgg cggatgtcgt gcagacccgg
      601 ctcgtgctgg aggacgctgt cgtcgcgcgt ctttctgctg acccgcggcg cgatctggcc
      661 ggcatccgcg gcatcctgac agccatggat gcggatgatc tcaccgcacc cgagttcctc
      721 gcgctcgacg ccgagctgca ctacgcactc gccgaggcct cagggaacac cgtcatcgcc
      781 gcgacgatgg cgggcctgcg caccgcgatc gagtcctatg tgctggaggg ctcggcgcgc
      841 atcgaggact gggacgccat ggcatcccgg ctgcgcacgg agcatcacgc gatcgtcgac
      901 gccatcgagg cgggtggggc ggatgccgca cgcgccctcg tccacgacca catcaccggc
      961 tactacaccg ccgccgggct cgtgcccggt gaccactgac acgcaccctg cagaggagag
     1021 agaccatggt ccagcgccag ctgcccaacc ccgctgagct gctcgagctc atgaagttca
     1081 agaagcccga gctggacggg cgcaagcgcc gtctcgacgc ggcgctgacg atctacgacc
     1141 tgcgcaccat cgccaagcgc cgcacgccga aggccgcctt cgactacacc gacggcgccg
     1201 ccgagggcga gctgtcgctg gcgcgcgccc gccaggcctt cgaggacgtc gagttccacc
     1261 ccggcatcct caagcccgcc cccaccgtcg acacgagcgt cgagatcctc ggcggtccgt
     1321 ccgcgcttcc cttcggcatc gcgcccaccg gcttcacccg cctcatgcag accgagggcg
     1381 agaccgcggg cgcgggggcg gcggccgccg caggtatccc gttcaccctg tccacgctgg
     1441 gcaccacctc gatcgagggc gtgaaggccg ccaacccgaa cggacgcaac tggttccagc
     1501 tgtacgtcat gcgcgaccgt gagatctcgt acgagctcac ccgccgcgcc gccgccgccg
     1561 gcttcgacac cctgcagttc acggtcgaca cccccgtcgc gggcgcccgc ctgcgcgaca
     1621 agcgcaacgg cttcagcatc ccgccgcagc tgaccctcgg caccatcatc aacgccatcc
     1681 cgcggccgtg gtggtggtac gacttcctca ccacgcccaa gctcgagttc gcctcgctca
     1741 gcaccaccgg cggcaccgtc ggcgagctgc tggacgccgc catggacccc acgatcagct
     1801 acgacgacct cgccgtcatc cgcgacctgt ggcccggcaa gatcgtcatc aagggcgtgc
     1861 agaacgtcga ggactcggtg cggctgaagg atgccggtgt cgacggcatc gtgctctcca
     1921 accacggcgg gcgtcagctc gaccgcgcgc ccatcccgtt ccgtctgctg ccgcacgtgc
     1981 gcaaggccgt gggtgacgac ttcaccgtga tggtggacac cggcatcatg aacggcgccg
     2041 acatcgtggc atccgtcgcc ctgggtgccg acttcaccct catcggccgc gcctacctgt
     2101 acggcctcat ggccggcggg cgtcagggcg tggaccgcac gatcgcgatt ctgcgcaccg
     2161 agatcgagcg cacgatgcgt ctgctgggcg tctccaccct ggccgagctc gagccgaagc
     2221 acgtcacgca gctggcgcgt ctggtgccgg tggccgaggg cgccgcggag tcggtcgtgc
     2281 gctgaggcgt gtcccagaag cggcgggtcg ggtcagccct ggaccgccgc ttctgagaca
     2341 tgacgcacgg tctgtctcag aacggaccgg tctgccgcct cgtgacccgc accttgtggg
     2401 acgcgactgc ggatgccgtc agcgggcgac gcggtccggc agacccgcga tgagctcgtc
     2461 gagcttgtcg gccgtgtcct cccagccctc cacggccacg gagggaacgc cgatcgcgag
     2521 cactgggtag tcgttgccgc cctcgtccag ccggtcgccg tagaagagca tgtcggccag
     2581 cgggatgccg gtgtggtcgg cgagctggcg catgccatag gccttgtcga tgccggcgcg
     2641 cgtgatgtcg atgctcgtcg agccgccgga gcgcacctcc aggcctggca ggcgcgcagc
     2701 gaccgcgtcg cggagaaggg cccgcttcgc gccgtccgga tcccaggcgt gcttcgcatc
     2761 gcgcggagcc tgctgcccga gcgccgagaa cgtgatctgc gacccgcggt cctcgaggat
     2821 ctcaccccag gtgcggctct cccacagacc cagccgctcg gcctcctcgc gcagcgcggt
     2881 gagcgcgtcg gacttctcct ggtcggtgag gtcgtgggcg tagaccgcgg cgaacgctgc
     2941 gccgtcgtgc cgcaggtagc gggtgccgca ggtgggcagc aggtgcagcc gggccagatc
     3001 ggatgccatc gtgtcggcga ggcgggcgat gacctgcgtg cggaactgat cctcgttgcc
     3061 gcccgagatg atcgccacgt ccacgcgctg cagcagtgcg cgcagcagat ccgcgatgcg
     3121 gacgtcgatc tcgcccttcg acggggcgag cgtgtcatcg aggtcgaagg cgaccaggcg
     3181 aggcgtgggc atagtgatta cagggtatcg gaggggagct gtgggggagc ggagggggct
     3241 gttcgacgtc cacgtcgcag ttcgcccgga atcctctggc tgcgcctccc cgggtcgcga
     3301 ttggtcccgg aatcggtgag ggataacgcg ctgaattgcg atctggaccg accgcggatg
     3361 ctgagagcga cacgaactgc gacccggagg cgcccgcggt cgggcgctga tgcgcgggat
     3421 cggcatgggt cggcggatgc gcggcagcgc gcctgcgcgg gagctgggg
//
LOCUS       DATMSX010000063        12696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180917,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000063 DATMSX010000000
VERSION     DATMSX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180917"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1145..1282
                     /locus_tag="VN041_01865"
     CDS             1145..1282
                     /locus_tag="VN041_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27825.1"
                     /translation="MDIRRQRELVAQGWIVVRLTQADLDDGGRILRESVRSAFEIRRS
                     S"
     gene            complement(1437..2759)
                     /locus_tag="VN041_01870"
     CDS             complement(1437..2759)
                     /locus_tag="VN041_01870"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HWU27826.1"
                     /translation="MSTPENWRFETKQIHAGAAPDPVTKARATPIYQTTSYVFDNADH
                     AANLFALAEFGNIYTRIQNPTQDVLEQRLAALEGGTGALVLSSGQAASTFAVLNIAQA
                     GDHIVSSSSIYGGTYNLFKYTLAKLGIEVTFVENQDDPAEWRAALRPNTKLFFAETIG
                     NPKINVLDIRTVADIAHEGGVPLIVDNTIATPFLIRPFEHGADIVVHSVTKFLGGHGT
                     TIGGAIIDGGSFEWSKNVEKFPGLTEPDPSYHGASYTTAVGDGLAYIIKARVQLLRDL
                     GSAIAPQSAWNLIQGIETLSLRVERHVQNAQEIAEWLENHDDVASVNYSGLPSSPWYA
                     KANEYAPKGVGAVLSFELKGGVEAGREFVNSLTLFSHLANIGDVRSLVIHPASTTHSQ
                     LSPEQQLTAGVTPGLVRLSVGLENIDDLKADLETALVAARRVAEAARA"
     gene            2896..3681
                     /locus_tag="VN041_01875"
     CDS             2896..3681
                     /locus_tag="VN041_01875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU27827.1"
                     /translation="MLNRRAVVTGASTGIGEAAVRALRAQGWDVVGVARRAERLEALA
                     AETGSSAVACDLTDPDAVAALIEELERTGPVHALVQVAGGARGTDSVENGSIDDWQWM
                     YDANVLSTQRLVAGILPLLRRAAEDSGHADTVFVTSTAAQTAYPGGAGYNAAKAAESM
                     LVKVLRQELNGEPIRVVEVAPGMVHTEEFTLNRLGGDRVAADAVYSGVEEPLLAEDVA
                     DVIAFALNAPRRVNLDLVTMRPVAQSAQHLLARGPLHVRTDVD"
     gene            3681..4379
                     /locus_tag="VN041_01880"
     CDS             3681..4379
                     /locus_tag="VN041_01880"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWU27828.1"
                     /translation="MPQTLAQLADAGQIDPGWAQALAPVQPLITELGDRLRAENAAGR
                     GYLPPGAHVLRAFQRPLADVRVLITGQDPYPTPGHPIGLSFAVDREVRPLPRSLGNIY
                     KERQDDLGIPPAPHGDLTAWSDQGVLLLNRVLTVRPGTPASHRGWGWEKVTELAIRTL
                     VARRKPLVAILWGADARGLKPLLEDTPIIESAHPSPLSASRGFFGSKPFSRANTMLES
                     MGAQPVDWRVEGEA"
     gene            4376..4879
                     /locus_tag="VN041_01885"
     CDS             4376..4879
                     /locus_tag="VN041_01885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27829.1"
                     /translation="MSALRIRPVADADLPAIRDIYNHYVRTSTVTYDEVESTEQTWAD
                     KSAKIADAGIPFLVAETEDGELLGYALGQQWSPKSAYRFTIENSIYLAPTAGGRGIGW
                     ALLAEFLDACRTAGLRQVIAVIADRGAEASIALHRKAGFTDAGRLTEVGLKFGEPQGV
                     FLLQKTL"
     gene            4892..5641
                     /locus_tag="VN041_01890"
     CDS             4892..5641
                     /locus_tag="VN041_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27830.1"
                     /translation="MTADPMHDVRAALIALKPVDAGIRSCVTLRSDSAFLNLLSTSDP
                     TRWAIVHSTGATWFSLVVDGHFTRDIIDVDLNADEVREHLAGLVKIAVPHLVGNSRLT
                     RRSGRRRLAVAVDDEEILLFAPLSRLLARLSPLRRWRIRRRAQLAAAGLLADHRLPAM
                     ASSWMIVHPGMWELLVDLDALQAGLAGSRTWRRTREGRMLRRELSGECLLLADALADL
                     DLVDVAVGLSDLGAQIAAFGSGRPIAVRGAG"
     gene            complement(5625..6413)
                     /locus_tag="VN041_01895"
     CDS             complement(5625..6413)
                     /locus_tag="VN041_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU27831.1"
                     /translation="MSLIEAEGLSRTFALPKRTLFESRRTSTALHHTDLTIDAGESVG
                     IIGESGSGKSTLVRLLLGLDRPTTGRVTVDGRDVDATASARSLHWLRRQTGLVFQDPY
                     ASLDPRMTAGRIIAEPLWALDIPGDHRARVREVLEQVGLEADMADRHPHEFSGGQRQR
                     IALARAIAHRPRVLVGDEPLSALDVTVRAQVLELIAQLQRDEGLTVIMVSHDIGVVQN
                     LADTVMVMKDGRIVERGPTDAVLQHPAEDYTRRLLAAIPVIPHP"
     gene            complement(6410..7231)
                     /locus_tag="VN041_01900"
     CDS             complement(6410..7231)
                     /locus_tag="VN041_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU27832.1"
                     /translation="MSLEVNDLVIEIDGRRVVDGVSFSVADGQRFGLIGESGSGKSLT
                     ALALLGLLPDAATASGSIRWNGTELIGMPDRELAKLRGDEIGMVFQEPRTALNPIRTV
                     GRQIAESVRIHERIGRREARERAVAEAARVRLPEPDSIVDRYPHQLSGGQRQRVAIAM
                     ALACRPKLLITDEPTTALDVTIQAEVLKLLLSLVEEHGMSLVFITHDLAVLSQVATHA
                     VVLEHGRIAEQGPVSTLLTAPSSPITRALLRDATATLWRPEGGHDEGHAPKGGVA"
     gene            complement(7228..8094)
                     /locus_tag="VN041_01905"
     CDS             complement(7228..8094)
                     /locus_tag="VN041_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27833.1"
                     /translation="MPRWLRSLLSSRTGLFGVFVVLLIAVTALVSLFWTPFDPMLSDI
                     RGRWAAPGWPHLLGTDGTGRDILSLVMAGARTTLFVAVGSGIVATLVGVALAALGALT
                     VRWVRETVAVLVDILIAFPVLMIAMMISAVWGGSLWVVVFSVGIGFGVSIARVTRPEL
                     RRVQQSDFVVAGRASGLTTGQNLVRHLLPNVAPVFIVQLSWSMAVAVLAEAGLSYLGF
                     GATLTEASWGTLLADLQRYIGVHPLTVVWPGLAITITVLGLNLLGDALREATDPTLSR
                     RSAEVHVPAVVA"
     gene            complement(8094..9047)
                     /locus_tag="VN041_01910"
     CDS             complement(8094..9047)
                     /locus_tag="VN041_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27834.1"
                     /translation="MLRYALTRGVLLIAGLLVSSVIIFFTLRVFPGDVAQLIAGTQAS
                     PEQVEAIRQSLGLDKPLLSQYTDWIGGILHGDLGTSQLSGASVGAELAEKARVTWPLA
                     LMSMTIAMLIAAPFGVLSAMLRGRAGGTALSVAAQAVAAIPVIWAGMMLVVVFAHWLG
                     WLPAQGFPRTGWSTPAKAFESLLLPAMTIGIVEGAMLMRFVRSATLQAAGQDFVRTAA
                     AKGLTRRQALIRHGIPAVGLSIVTVLGVQVAGIIVGSVVVEQLFTLPGIGRMLVADVG
                     TRDLVKVQSELLVLTGFVLVIGFLVDLVHHAIDPRQREEAG"
     gene            complement(9049..10578)
                     /locus_tag="VN041_01915"
     CDS             complement(9049..10578)
                     /locus_tag="VN041_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU27835.1"
                     /translation="MSPFRSTRRSARLAAIGILAIGALILTACGGSSEPQTTSTGKAD
                     PKAGLTVGLVLEPTNLDIRHTSGAALEQILVDNIYEGLVTRTQDNKIDDRLAKSHEIS
                     PDGLTYTFTLNEGISFHDGTALTSADVVSSYETVKSDATVQGNAEFAGVTAIIAPDPT
                     TVQITLAKPDQNFLFNLTGPAGLVFKTGDTTDLKTAENGTGPFTLQRWAKGSSITLSR
                     NDDYWGKKAGLAEVTFQYIPDFTAGVNAALDGSVDVLTAVDPNLVPQLKDSGDFTITT
                     GRTTDKATLAFNNAKAPLNDKRVREALRLAVDHEALVEAVGAGQPLYGPIPELDPGYE
                     NLSKLVPYDPDKAKALLKEAGVEKLDLSLTIPSFYGTTVAQVLVSDYAKIGVSLEVDR
                     VEFATWLEDVYKNHDYELSFVLHVEPRDFSNWANPEYYFGYDNKQVQDLYAQSQAELD
                     PAKSADLLAQAARIVSEDDAADWLYNGATLTALTAGVKGFPKDSINSRIDLAGVTKTA
                     G"
     gene            complement(10623..11537)
                     /locus_tag="VN041_01920"
     CDS             complement(10623..11537)
                     /locus_tag="VN041_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU27836.1"
                     /translation="MAVAQDTSEFRYLPTQAAILGTTVPAAERISLTLPDGRILSALR
                     FGDGDPEVTLLHGAGLNAHTWDTVQLLLDRPALAIDLAGHGDSSWREDLDYSPASLAA
                     DVAIAMSEWTRDPQVLIGHSLGGLTASVVAARNPQLVRELILVDIVPGMDTDAAPAVL
                     RDFYQVTEFANREDAVDRAQAFGFGGSREDTERGVFFNTRVRADGRVEWKHHFAHIIG
                     QAFDALNAANAKAAEADPWADLEAVTAPVTLVRGARGFLADTDAAAFERRLPNATAIT
                     VDAGHNVQETDPAFLASLASDALTRPSR"
     gene            11698..12402
                     /locus_tag="VN041_01925"
     CDS             11698..12402
                     /locus_tag="VN041_01925"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWU27837.1"
                     /translation="MTVIRIPGVNNLRDVGGIPVGSARVRSGALLRSGHLARVTPEGQ
                     ALLRDRIRRVVDLRDDTEVRSEPSALRDVPTTRVPLFLGSVGSFFEQDMDLAGMYGHL
                     VDDCSSRLADAVRIISEGEPALVHCTVGKDRTGVIVALALSAVGADREAVVADYALTA
                     SQLPAERNRAIAEYFRAHMPDARNAIQLATESPAPVMAALLGDLDRRYGSVAAYLLEA
                     GLTQAELDALADALIE"
     gene            12488..>12696
                     /locus_tag="VN041_01930"
     CDS             12488..>12696
                     /locus_tag="VN041_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWU27838.1"
                     /translation="MSTAETTASARAARRASRRPQVQHLITADENSLAELEVLLATLP
                     LCAVGRVFIEVADQDDVSVVSAPPR"
BASE COUNT         1919 a         4177 c         4629 g         1971 t
ORIGIN      
        1 gtcgcgccag gcgccggtgg cggggggacg ggcgcgcagc agtcgctggt cggcctcggt
       61 cacgggcgcc gagggcaccg tgtcctctga ggtcgtctgc cagtccatgt gtcccattct
      121 cccgggtgcc cgcccgcgcg agcagcaggt tacgcccccg ttgtctcggc ccttcgcctg
      181 cccacccgcc ggaggaagag gaaaacgttc gtcaatccca gccggaaccg acgttttcct
      241 cctcccagca gtacaaggcc ttcaccctgc acaggccgcg ctgtatcccg ggttgtcccc
      301 gaagcccggg aatgcgccgc gactcggcct gctcgccccg ggagcatgga cggatgcgaa
      361 tacgagggcc tcttccgtcc agactgggcg tccggttcac gatccaggag gccgcacggg
      421 ccggagtcgg gcgttcgcga cacgacgctc ctgatcttct ccgcccgttc cccggcatcc
      481 gcagtgtcac gccggtcgag accttcagcg agcgggtcag cacgtatcac cggcggatga
      541 agcccgggca ccgctacgtg gggcggaccg cgatgcgtct gtggggactg cctctgccct
      601 gggaatggaa tcgccgggag tctctgcagg ttgcagtccc gcccgatgca gcaccgcctc
      661 ggactgccgg cgtcatgggg cgcaggctcg ccccgaaccg ggcggagaca ctgattctgc
      721 agggcgttcg ggtcgtcgac gcgatcgcgg cgttcttctc gaccgctgcc gaacttgatc
      781 tggggtatgc catcgccgtc gcggacgcac tcctgaccac cgcggacggc tatccggggc
      841 tcggtccggg cagacccctg gcgacacgcg agtcgatcgc gcgaaggctc accgactggg
      901 gcaggttccc cggatcggcg acggtgcgag aagcgttgaa gcatgcccgg gagaaggtgg
      961 aatcacccaa ggagaccgag acgcggctgc tgctcgtgaa tgcgggcatg cgtgagcccg
     1021 agatccagtt cgaggtgagg gcggggcgtc gcctggtcgc gaggacggat ctggcgtacc
     1081 cggagctgaa gatcgcgatc gagtacgaag gcgacgtcat cggcgcagca aggagcagtg
     1141 gcgcgtggac atccgccgcc agcgcgagct cgtggctcag ggatggatcg tcgtccggct
     1201 cacccaggcc gacctcgatg acggcggacg gattctgcgg gagagcgtcc gctcggcgtt
     1261 cgagattcgc aggagctcat gaaaggagga gaacgtcggg tatccgccag gttggtcgac
     1321 ggtttcctcc cgaaccggca ccagacgagg gctcgatgcc ggacgggagc ccgcacgcac
     1381 ggatgccccg gaccgcgcgg tccggggcat ccgtgtctct gaggaggaga gtgcgatcag
     1441 gcgcgagcgg cctcggcgac gcgacgggcg gcgaccagcg ccgtctccag gtccgccttg
     1501 aggtcgtcga tgttctccag gccgaccgac aggcgcacca ggcccggcgt gacgccggcg
     1561 gtgagctgct gctcgggcga gagctgggag tgggtggtgg aggccgggtg gatgaccagg
     1621 gagcgcacgt caccgatgtt ggcgaggtgg ctgaacagcg tgaggctgtt cacgaactcg
     1681 cgaccggcct ccacaccgcc cttgagctcg aaggacagca ccgcgccgac gcccttcggg
     1741 gcgtactcgt tggccttggc gtaccagggc gaggacggca gacccgagta gttcaccgag
     1801 gcgacgtcgt cgtggttctc gagccactcg gcgatctcct gtgcgttctg cacgtgacgc
     1861 tcgacgcgca gcgagagcgt ctcgatgccc tggatcaggt tccacgcgct ctgcggggcg
     1921 atcgcggagc cgaggtcgcg cagcagctgc acgcgggcct tgatgatgta cgcgagaccg
     1981 tcgccgacgg cggtggtgta gctcgcgccg tggtaggacg ggtccggctc ggtgaggccg
     2041 gggaacttct cgacgttctt cgaccactcg aaggagccgc cgtcgatgat cgcgccgccg
     2101 atggtggtgc cgtggccgcc gaggaacttg gtgaccgagt ggacgacgat gtcggcgccg
     2161 tgctcgaacg ggcggatgag gaacggggtg gcgatcgtgt tgtcgacgat cagcggcacg
     2221 ccaccctcgt gggcgatgtc ggcgaccgtg cggatgtcga gcacgttgat cttcgggttg
     2281 ccgatggtct cggcgaagaa cagcttcgtg ttcgggcgga gcgcggcgcg ccactcggcc
     2341 gggtcgtcct ggttctcgac gaaggtgacc tcgatgccga gcttggcgag ggtgtacttg
     2401 aagaggttgt aagtgccgcc gtagatcgag ctggacgaca cgatgtggtc gccggcctga
     2461 gcgatgttca gcacggcgaa cgtggacgcc gcctggccgc tggagagcac gagggcaccg
     2521 gtgccgccct cgagcgcggc gaggcgctgc tcgagcacgt cctgggtggg gttctggatg
     2581 cgggtgtaga tgttcccgaa ctcggccagg gcgaacaggt tcgcggcgtg gtcggcgttg
     2641 tcgaacacgt aggaggtcgt ctggtagatc ggcgtggcgc gcgccttcgt gaccgggtcg
     2701 ggtgccgcgc cggcgtggat ctgcttggtc tcgaagcgcc agttctcggg tgtggacatg
     2761 aggttctccc agagtgagtg tggtcgtgcg gccggatgcc tggaagcgag gttacggagc
     2821 gagcacgacg ccggacaacc gccgggaaat gttacgtaac acagcgaagc gccccgaaag
     2881 ccgtacggtg gatgcatgct gaacagacgt gccgtggtca caggggcgag taccggtatc
     2941 ggagaggccg cagtgcgtgc gctgcgtgcg cagggctggg acgtcgtcgg cgtcgccagg
     3001 cgggcggagc gcctggaggc gctcgccgcg gagacgggat cgtcggcggt cgcctgcgat
     3061 ctgaccgatc cggatgccgt ggccgcgctc atcgaggagc tcgagcgcac cggtcccgtc
     3121 cacgcgctcg tgcaggtcgc cggcggcgcg cggggcaccg acagcgtcga gaacggctcg
     3181 atcgacgact ggcagtggat gtacgacgcg aacgtgctgt cgacgcagcg gctcgtcgca
     3241 ggcatcctgc cgctgctgcg ccgcgccgcg gaggactccg gccacgccga cacggtcttc
     3301 gtgacgtcga cggccgcgca gaccgcctat ccgggcggcg ccggatacaa cgcggcgaag
     3361 gctgcggagt cgatgctcgt caaggtgctg cgtcaggagc tgaacggcga gcccatccgc
     3421 gtcgtcgagg tcgctcccgg catggtgcac accgaggagt tcaccctcaa tcgtctcggc
     3481 ggcgaccggg tcgccgcgga tgccgtgtac tcgggcgtcg aggagccgct gctggccgag
     3541 gacgtcgccg acgtgatcgc cttcgcgctg aacgcgccgc gtcgcgtgaa cctggatctc
     3601 gtgaccatgc gtccggtggc gcagtccgcg cagcacctgc tggcgcgcgg gccgctgcac
     3661 gtgcgcaccg acgtcgactg atgccgcaga cgctcgccca gctggccgac gccgggcaga
     3721 tcgatcccgg gtgggcacag gcgctcgcac ccgtgcagcc gctgatcacc gagctggggg
     3781 accggctgcg cgccgagaac gccgccggtc gcggctacct gcccccgggg gcccacgtgc
     3841 tgcgtgcctt ccagcggcca ctggcggacg tgcgcgtgct gatcacgggt caggacccgt
     3901 acccgacgcc ggggcatccg atcggcctgt cgttcgcggt cgaccgcgag gtgcggccgc
     3961 tgccgcgcag cctcgggaac atctacaagg agcgccagga cgatctcggc atccccccgg
     4021 ctccacacgg cgacctgacc gcctggagcg accagggcgt gctgctgctg aacagggtgc
     4081 tgaccgtgcg gcccggcacc ccggcctcgc accgcggctg gggctgggag aaggtcaccg
     4141 agctcgcgat ccgcacgctg gtcgcgcgcc ggaagccgct ggtcgcgatc ctctggggag
     4201 cggatgcccg gggcctcaag cccctgctgg aggacacccc gatcatcgag tccgcccacc
     4261 cctctccgct gtcggcgagc aggggcttct tcgggtcgaa gccgttctcc cgggcgaata
     4321 cgatgctgga gtcgatgggc gcgcagcccg tcgactggcg tgtggaggga gaggcatgag
     4381 cgcgctgcgc atccggccgg tggcggacgc cgatctgccg gcgatccgcg acatctacaa
     4441 ccactacgtg cgcacctcga cggtcacgta cgacgaggtc gagtccacgg agcagacctg
     4501 ggcggacaag tccgcgaaga tcgcggacgc gggcatcccg ttcctggtgg ccgagaccga
     4561 ggacggcgag ctcctcggct acgcgctcgg ccagcagtgg tcgccgaagt cggcgtaccg
     4621 gttcacgatc gagaactcga tctacctggc cccgacggcc ggtgggcggg ggatcgggtg
     4681 ggcgctgctc gccgagttcc tggacgcctg ccgcacggcg ggtctgcgtc aggtcatcgc
     4741 cgtgatcgca gaccgtgggg ccgaggcctc catcgccctg caccgcaagg caggcttcac
     4801 cgatgcgggg cggctcaccg aggtcggtct gaagttcggc gagccgcaag gagtgttcct
     4861 gctgcagaag accctgtgat cgcggggttc tgtgaccgcc gatccgatgc atgacgtccg
     4921 cgccgccctc atcgctctga agccggtcga cgcaggcatc cgttcgtgcg tcacgctgcg
     4981 gagcgactcg gcgttcctga acctcctcag caccagcgac cccacacggt gggcgatcgt
     5041 gcactcgacg ggagcgacct ggttctccct cgtcgtcgac ggtcatttca ccagggacat
     5101 catcgatgtc gacctgaacg cggatgaggt gcgcgagcac ctcgcgggcc tggtgaagat
     5161 cgcggtcccg cacctggtcg gcaacagccg gctgacgcgc cggagcgggc ggcgtcgtct
     5221 cgccgtcgct gtcgacgacg aggagattct gctgttcgcc ccgctcagcc gtctgctcgc
     5281 gcggctctca ccgctgcgac ggtggcgcat tcgccggagg gcgcagctcg cggctgccgg
     5341 cctgctggcc gatcatcgcc tgccggctat ggcgtcgagc tggatgatcg tgcatccagg
     5401 gatgtgggag ctgctcgtcg atctcgatgc cctgcaggca ggacttgccg gctcccggac
     5461 gtggcggcgc acgcgcgagg gacgaatgct gcgacgagag ctgtccgggg agtgtctcct
     5521 gctggcggat gcccttgctg acctcgacct cgtcgatgtc gcggtcggtc tctccgacct
     5581 cggtgcccag atcgccgcgt tcggatccgg acgcccgatc gcggtcaggg gtgcgggatg
     5641 accgggatcg ctgcgagcag ccgccgcgtg tagtcctcgg cggggtgctg cagcacggca
     5701 tccgtcggtc cgcgctccac gatccgtccg tccttcatca ccatgaccgt gtcggcgagg
     5761 ttctgcacca cgccgatgtc gtgcgagacc atgatgaccg tcaggccctc gtcgcgctgc
     5821 agctgcgcga tcagctcgag cacctgtgcg cggacggtca cgtccagcgc tgaaagaggc
     5881 tcgtcgccga cgaggacgcg ggggcgatgc gcgatcgcgc gggccagggc gatgcgctgg
     5941 cgctgccctc cggagaattc gtggggatgc cggtctgcca tgtcggcctc caggcccacc
     6001 tgctcgagca cctcgcgcac gcgggcacgg tggtcaccgg ggatgtcgag ggcccagagc
     6061 ggctcggcga tgatccggcc cgccgtcatc cgcggatcca gcgacgcgta cgggtcctgg
     6121 aagaccagcc cggtctgccg gcgcagccag tgcagcgagc gggcggatgc cgtggcatcc
     6181 acgtccctgc cgtccaccgt cacccggccg gtcgtcggcc ggtcgagccc cagcagcagg
     6241 cgcaccaggg tcgacttccc ggagccggac tcgccgatga tgccgaccga ctcgccggca
     6301 tcgatcgtca gatcggtgtg atgcagggcc gtgctcgtgc ggcgcgactc gaagagggtg
     6361 cgcttcggaa gggcgaacgt gcggctgagg ccctcagcct cgatgagact catgcgacgc
     6421 ctcccttcgg cgcgtgaccc tcgtcatgcc cgccctcggg acgccacagc gtcgcggtcg
     6481 cgtcgcgcag cagcgcccgg gtgatcgggg acgacggcgc ggtcagcagc gtcgagacgg
     6541 gtccctgctc ggcgatgcgc ccgtgctcga gcaccaccgc gtgcgtcgcc acctgcgaca
     6601 gcacggcgag atcgtgcgtg atgaacacca gcgacatgcc gtgctcctcc accagtgaca
     6661 ggagcagctt cagcacctcg gcctggatcg tgacgtcgag ggcggtggtg ggttcgtcgg
     6721 tgatcagcag cttcggccgg caggcgagcg ccatcgcgat cgcgacgcgc tggcgctggc
     6781 cgccggacag ctgatggggg tagcggtcga cgatggaatc cggttccggg agccggacgc
     6841 gagcggcttc ggcgacggcc cgctcgcggg cctcgcggcg tccgatgcgc tcgtggatgc
     6901 gcaccgactc cgcgatctgc cggccgacgg tgcggatcgg gttcaacgcg gtgcgcggct
     6961 cctggaagac catgccgatc tcatcaccgc gcagcttcgc gagttcgcgg tcgggcatcc
     7021 cgatcagctc ggtgccgttc caacggatgc tgccgctcgc cgtcgccgca tccgggagca
     7081 gacccagcag tgcgagcgcg gtcagggact tgccggatcc cgattcgccg atcaggccga
     7141 agcgctggcc gtcggcgacc gagaacgaga cgccgtcgac cacacgacgg ccgtcgatct
     7201 cgatgaccag gtcgttcacc tcgaggctca tgcgaccacc gccgggacat gcacctctgc
     7261 gctgcggcgg ctcagcgtcg ggtcggtggc ctcgcgcagg gcgtcgccga gcaggttcag
     7321 cccgagcacc gtgatcgtga tggcaaggcc cggccagacg acggtgagcg gatgcacgcc
     7381 gatgtaccgc tgcagatccg ccagcagcgt cccccaggac gcttcggtga gggtcgctcc
     7441 gaagcccagg tacgacaggc cggcctcggc gagcacggcg accgccatcg accaggacag
     7501 ctgcacgatg aacaccggtg ccacgttcgg cagcaggtgc cggacgaggt tctgccctgt
     7561 ggtgagcccg ctcgcacggc cggccacgac gaagtcgctc tgctgcaccc ggcgcagctc
     7621 ggggcgggtc acgcgggcga tgctcacacc gaacccgatg cccaccgaga acacgacgac
     7681 ccacagggag ccgccccaga cggcggagat catcatggcg atcatcagca cggggaacgc
     7741 gatgaggatg tccaccagga ccgcgaccgt ctcgcgcacc cagcgcacgg tgagggcgcc
     7801 cagggcggcg agggcgacac cgacgagggt cgcgacgatg ccggaaccca cggcgacgaa
     7861 aagggtcgta cgcgcaccgg ccatgacgag gctgaggatg tcccgcccgg tgccgtcggt
     7921 acccagcaga tgcggccagc cgggcgccgc ccaccggccg cggatgtcgg agagcatcgg
     7981 gtcgaacggg gtccagaaca gcgagaccag cgctgtcacg gcgatcagca gcacgacgaa
     8041 cacgccgaac agtcccgtgc gcgacgagag cagggaacgc aaccagcggg gcatcatccg
     8101 gcctcctcgc gctgtcgcgg gtcgatggcg tgatggacga ggtcgaccag gaagccgatc
     8161 accagcacga agccggtcag caccagcagc tcgctctgca ccttcacgag atcgcgcgtg
     8221 ccgacgtcgg cgaccagcat gcgcccgatg ccggggagcg tgaacagctg ctccacgacg
     8281 accgagccca cgatgattcc cgcgacctgc acgcccagca cggtcacgat ggagagtccc
     8341 acggcaggga tcccgtgccg gatcagcgcc tggcggcggg tgagtccctt ggcggccgca
     8401 gtacgcacga agtcctgccc ggcggcctga agggtcgcgc tgcgcacgaa gcgcatcagc
     8461 atggcgccct cgacgatccc gatggtcatg gccggcagca gcagcgactc gaacgccttg
     8521 gccggcgtcg accagccggt gcgggggaac ccctgggcgg gcagccagcc cagccagtgc
     8581 gcgaagacga cgaccagcat catgccggcc cagatgaccg ggatcgcggc gaccgcctgc
     8641 gctgcgacgc tcaacgcggt gccgccggcg cgaccccgca gcatcgcgga gagcacgccg
     8701 aagggcgctg cgatcagcat cgcgatggtc atggacatca gggccaacgg ccaggtgacc
     8761 cgcgccttct cggcgagctc ggcgcccacc gaggccccgc tgagctgaga ggtgcccagg
     8821 tcgccgtgca ggatgccgcc gatccagtcg gtgtactgcg agagcagcgg cttgtcgagt
     8881 ccgagcgact ggcggatcgc ctcgacctgc tcggggctcg cctgcgtgcc ggcgatcagc
     8941 tgcgcgacgt cgccggggaa gacccgcagg gtgaagaaga tgatcacgct cgacacgagg
     9001 agtcctgcga tgagcaggac tcctcgtgtg agcgcgtagc ggagcatgtc agccggcggt
     9061 cttggtgacg cccgcgaggt cgatgcgcga gttgatcgaa tccttcggga agcccttgac
     9121 gcccgcggtc agtgcggtca gtgtcgcgcc gttgtacagc cagtccgcag cgtcgtcctc
     9181 ggagacgatc cgcgcggcct gcgcgagcag atcggccgac ttcgccgggt cgagctcggc
     9241 ctgcgactgc gcatacaggt cctgcacctg cttgttgtcg tagccgaagt agtactcggg
     9301 gttcgcccag ttgctgaagt cgcggggctc gacgtgcagc acgaagctca gctcgtagtc
     9361 gtgattcttg tagacgtcct cgagccaggt ggcgaactcg acgcggtcga cctcgagcga
     9421 gacgccgatc ttggcgtagt cggagacgag cacctgagcg acggtcgtgc cgtagaacga
     9481 cgggatcgtc agggagaggt cgagcttctc tacgccggcc tccttgagga gcgccttggc
     9541 cttgtcgggg tcgtagggga ccagcttgga caggttctcg tagccagggt cgagctccgg
     9601 gatcggaccg tacaggggct ggccggcacc gaccgcctcg acgagagcct cgtggtcgac
     9661 cgccaggcgc agcgcctcgc gcacgcgctt gtcgttcagc ggagccttcg cgttgttgaa
     9721 ggcgagcgtg gccttgtcgg tggtgcgccc cgtcgtgatg gtgaagtcgc cgctgtcctt
     9781 cagctgcggg accaggttcg ggtcgacggc ggtgagcacg tcgacgctgc cgtccagagc
     9841 cgcgttcacg ccggcggtga agtccgggat gtactggaac gtgacctcgg cgagcccggc
     9901 cttcttgccc cagtagtcgt cgttgcgcga gagcgtgatc gaactgccct tggcccagcg
     9961 ctgcagtgtg aaaggacccg tgccgttctc cgcggtcttc agatcggtgg tgtctccggt
    10021 cttgaagacg aggccggcag gtcctgtgag gttgaagagg aaattctgat ccggcttggc
    10081 cagcgtgatc tgcaccgtcg tcgggtcggg cgcgatgatc gccgtgacgc cggcgaactc
    10141 ggcgttgccc tgcaccgtgg catccgactt cacggtctcg tacgacgaga cgacatcggc
    10201 cgaggtgagg gcggtgccgt cgtggaagga gatgccctcg ttgagcgtga aggtgtaggt
    10261 gaggccgtcg ggcgagatct cgtgggactt cgccaggcgg tcatcgatct tgttgtcctg
    10321 cgtgcgcgtg acgaggcctt cgtagatgtt gtcgacgagg atctgctcga gtgctgcgcc
    10381 gctggtgtgg cggatgtcga ggttggtggg ctcgagaacc aggccgaccg tgagaccggc
    10441 cttggggtcg gcctttcctg tcgaggtggt ctgcggctcg gacgatccgc cgcaggcggt
    10501 gaggatcagg gcgccgatgg cgaggatgcc gatggccgcc agacgggccg atcgtcgggt
    10561 gctgcggaag ggagacacgg tgtttcctct cggaggggtg ctgtgagacg ccgcggggct
    10621 ggtcagcggg aaggtcgggt cagggcgtcg gatgcgaggg atgcgaggaa ggccgggtcg
    10681 gtctcctgca cgttgtggcc ggcgtcgacg gtgatcgcgg tggcgttcgg gaggcgccgc
    10741 tcgaacgcgg ccgcgtcggt gtcggcgagg aagccgcgcg cacctcggac gagggtgacg
    10801 ggcgcggtga ccgcctcgag atccgcccac gggtcggcct cggcggcttt cgcgttggcg
    10861 gcgttcagtg cgtcgaaggc ctgcccgatg atgtgcgcga agtggtgctt ccactccacg
    10921 cgcccgtccg cgcgcacgcg cgtgttgaag aagacaccgc gctcggtgtc ctcgcgggaa
    10981 ccgccgaagc cgaacgcctg cgcccggtcg accgcatcct cgcggttcgc gaactcggtc
    11041 acctggtaga agtcgcggag aacggcgggc gcggcgtcgg tgtccatccc gggaacgatg
    11101 tcgacgagga tgagctcgcg gacgagctgc ggattccgcg cggccacgac ggatgccgtg
    11161 agcccgccga gcgagtgccc gatcagcacc tgcgggtcgc gcgtccactc cgacatcgcg
    11221 attgcgacgt cggcggcgag ggaggcgggg gagtagtcga ggtcctcgcg ccaggaggaa
    11281 tcgccgtgcc cggcgagatc gatcgcgagg gcgggccggt ccagcagcag ctggaccgtg
    11341 tcccaggtgt gtgcgttcag gccggcgccg tgcaggaggg tcacctccgg gtctccgtcg
    11401 ccgaagcgca gggcgctgag gatgcgaccg tcgggcagcg tcagcgagat gcgctcggcg
    11461 gcgggtacgg tggtgccgag gatcgccgcc tgcgtgggca ggtaccggaa ctcgctggtg
    11521 tcttgtgcca cggccatatt ctcctatccg gatgtggctc acaggaaatg cgagatcatt
    11581 acgacaagta atcttcggta atcaatggac ataaagaaga catgtgacca ctttggcttg
    11641 tctttctgtc ttcgccggtg tcggaccggg tcgcgctccc tcgcctaggc tggaacggtg
    11701 acggtcattc gcattcccgg cgtgaacaac ctgcgcgacg tcggcggcat tcccgtcggt
    11761 tcggcgcgcg tgcggtcggg tgcgctgctg cggtcgggcc acctcgcgcg ggtcacgccg
    11821 gaagggcagg cgttgctgcg cgaccgcatc cggcgcgtgg tcgacctgcg ggacgacact
    11881 gaggtgcgct cggagccttc ggcgctccgt gacgtcccga cgacgcgcgt cccgctgttc
    11941 ctcggctcgg tcggatcgtt cttcgagcag gacatggatc tggccggcat gtacggccat
    12001 ctggtcgacg actgctcgtc gcgactggcg gatgccgtgc gcatcatctc cgagggggag
    12061 cccgcactcg tgcactgcac ggtcggcaag gaccgcaccg gggtcatcgt cgcgctggcg
    12121 ctgagcgcgg tcggagcgga ccgcgaggcc gtggtcgcgg attacgcgct gacagcctcg
    12181 cagctcccgg ctgagcgcaa tcgcgcgatc gcggagtact tccgcgccca catgcccgat
    12241 gcgcgcaacg cgatccagct cgccaccgag tcgcccgcgc cggtcatggc cgcgctcctc
    12301 ggcgatctcg accgccgcta tggctccgtc gccgcctacc tcctcgaggc gggtctcacg
    12361 caggccgagc tggatgccct cgcggacgcg ctgatcgagt gagcgtgtgc tccgacaggc
    12421 ttagcagccc aaggctggga gccgcatagg ttaggcaacc cttcctcggt gctacgatga
    12481 agacatcatg agcactgccg agacgaccgc gagcgcacgc gccgcccgcc gggcgtcccg
    12541 tcgcccgcag gtgcagcacc tgatcaccgc cgacgagaac tcgctcgccg agctggaggt
    12601 cctgctcgcg acgctgccgc tgtgcgccgt gggccgtgtg ttcatcgagg tcgccgacca
    12661 ggacgacgtc agcgtcgtct cggcgccgcc gcgcat
//
LOCUS       DATMSX010000064         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_86140,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000064 DATMSX010000000
VERSION     DATMSX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86140"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VN041_01935"
     CDS             complement(<1..962)
                     /locus_tag="VN041_01935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWU27839.1"
                     /translation="MNDLQEISGFSLTRRNLFKAGGAFGIAAAFTGAQVSGLTAAEQA
                     LAGAPRDAIKLNVLFIGAHPDDEAGNLGVFGQWNEYLGMKAGVITVTRGEGGGNAVGL
                     EEGPPLGMMREAEERKAVGYAGIENVFNLDGLDFYYTASAPLSYQVWDGAAVLSRIVR
                     VVRATRPDVIVTMNPSAVEGNHGNHQQAAMFAVEAYLAAGDPQRYPEHFAEGFEPWQP
                     RRILRSGANGTGAAGEGGVAAGYTPTVASDVVFGCWNGTPSQRNGKRWSEMLDLARWA
                     YVTQGWAEFAPGTTDPAKIANSWFTVIHSRSPLADPRSGGDAALR"
     gene            complement(1124..>1931)
                     /locus_tag="VN041_01940"
     CDS             complement(1124..>1931)
                     /locus_tag="VN041_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU27840.1"
                     /translation="ARLRIARGEPAHSVLDRAFDCARDLVGRHGGIGSIGVSTPGIVF
                     EDRVELAPNVIGWSDLELGARLRDAFAVTALTIENDVKAAALAECREGRLRGVDIGLY
                     VNLGTGIAISPVIGGAVLRGAHGAAGEVGYGIVGPLHERGRPVITLEEFAGGLGLDGR
                     VAASSEVTATDTAELIAECADSDAAARMWSEAVDEIARHLITAALTVDPSRIAIGGGM
                     TRAGGALLDPLTAALDSALPYPPEVVLSDFGPDASLRGAVMLARESQGVR"
BASE COUNT          297 a          692 c          667 g          275 t
ORIGIN      
        1 cgcagcgccg cgtcgccgcc ggagcgcggg tcggcgagcg gcgagcggga gtggatgacc
       61 gtgaaccagg agttcgcgat cttcgccgga tccgtcgtgc ccggcgcgaa ctcggcccag
      121 ccctgcgtga cgtatgccca gcgcgcgagg tcgagcatct cggaccagcg cttgccgttg
      181 cgctgactcg gcgtaccgtt ccaacagccg aacacgacgt ccgaggccac ggtcggggtg
      241 tagcccgcgg cgacgccgcc ctcgcccgcc gcacccgtgc cgttggcgcc ggagcgcagg
      301 atacggcgcg gctgccaggg ctcgaagccc tcggcgaagt gctcgggata gcgctgcggg
      361 tcgccggcgg cgaggtaggc ctcgaccgcg aacatcgcgg cctgctgatg gttgccgtgg
      421 ttgccctcga ccgccgacgg gttcatcgtc acgatgacgt cggggcgggt ggcgcgcacg
      481 acgcggacga tgcggctgag cacggcggcg ccgtcccaca cctggtacga cagtggcgcg
      541 ctcgccgtgt agtagaagtc gaggccgtcg aggttgaaca cgttctcgat gccggcgtag
      601 ccgaccgcct tgcgctcctc ggcctcgcgc atcatgccga ggggcggacc ctcctcgagg
      661 ccgaccgcgt tgccaccgcc ctcaccgcgg gtcacggtga tgacgccggc cttcatgccc
      721 aggtactcgt tccactgacc gaagacaccg aggttcccgg cctcgtcgtc ggggtgggcg
      781 ccgatgaaga gcacgttgag cttgatcgca tcgcgcgggg cgcccgcgag cgcctgctca
      841 gcggcggtga ggcccgagac ctgtgcgccg gtgaaggcgg cggcgatgcc gaaggcgcct
      901 cccgccttga acaggttccg acgtgtcagg gagaagccgg agatttcctg aagatcgttc
      961 attgggggac cagacctttc acacgggcgc acctcgatgt ggcgctccat gccgtcgatg
     1021 agagggggag caacacttat tttctgaaca gaaataagtg ttgctcccat tccactggtc
     1081 ggcgcgcggg tcgtcaacgg cggaaaataa gtccggatgg gggctaacgc acgccctggc
     1141 tttcccgcgc cagcatcacg gcaccacgca gcgaggcgtc cggcccgaag tccgacagca
     1201 ccacctcggg cgggtagggg agcgcactgt cgagggctgc ggtcagcggg tcgagcaggg
     1261 ccccgcccgc ccgcgtcatg ccgcccccga tcgcgatgcg cgaggggtcg acggtcagcg
     1321 ccgccgtgat gaggtgccgc gcgatctcat ccacggcctc ggaccacatc cgtgccgccg
     1381 catccgaatc ggcgcactcc gcgatgagct cggcggtgtc cgtcgccgtc acctcggacg
     1441 aggcggccac ccgtccgtcc agtcccaggc cgccggcgaa ctcctccagc gtgatcaccg
     1501 gccgcccgcg ttcatggagg gggcccacga tgccgtagcc gacctcgccg gccgcaccgt
     1561 gcgcgccccg gagcaccgcg ccgccgatca ccggcgagat cgcgatcccc gtgccgagat
     1621 tcacgtacag gccgatgtcc acaccccgga ggcgcccctc gcggcactcc gccagagccg
     1681 ccgccttcac atcgttctcg atcgtcagag cggtcaccgc gaaggcgtca cgaagccgcg
     1741 cgcccagttc gagatccgac catccgatca cgttcggagc cagttcgacg cgatcctcga
     1801 acacgatgcc gggcgtggag acgccgatcg acccgatgcc tccatggcgt ccgacaagat
     1861 cgcgggcgca gtcgaaggcg cggtcgagca cgctgtgcgc cggctcaccg cgcgcgatgc
     1921 gcagccgcgc c
//
LOCUS       DATMSX010000065         8975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124066,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000065 DATMSX010000000
VERSION     DATMSX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124066"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VN041_01945"
     CDS             <1..360
                     /locus_tag="VN041_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWU27841.1"
                     /translation="VTRILAIIAELNNDDKGLIWPASVAPFDVQVVAAGRDQVAFDVA
                     EDIANQLEAARLDVLYDDRVKVSPGVKFGDAELVGVPKIVIVGRGAADGQVELWDRAT
                     GEREALSAADAVARLTR"
     gene            500..1534
                     /locus_tag="VN041_01950"
     CDS             500..1534
                     /locus_tag="VN041_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWU27842.1"
                     /translation="MTDATLNDDLRHELTTLLDRAGIHADRGLIARMLQTALKLGVEH
                     TDRLDLKIASAALSEMHDAFRLFQPFHQVPKVTVFGSARTAQDDPLYIQARDVAAALA
                     SDGWMVVTGAGPGIMQAAAEGAGAKMSLGVSIRLPFEERMNTIIAQDAQVVSMKYFFT
                     RKLMLMKESRGFICLPGGFGTMDEMFELLTLQQTGKAEPMPIVLLDAQGGSFWTGLKT
                     FVDEHLAPAGVISRDDLDRVLITDSVSEAVANITGFWRNYDSLRWVGDRLVLRLVHEP
                     TDADIDSLNERFGGMCAEGRIERTDPLDVELADGDAVELPRIVLNLAQRSVGSLFALI
                     RAINELPSAR"
     gene            complement(1596..1943)
                     /locus_tag="VN041_01955"
     CDS             complement(1596..1943)
                     /locus_tag="VN041_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27843.1"
                     /translation="MKRMALGATVALVFVLVGAGTAYAGEVTGNGKEAQGANHASSEC
                     AFSGQDTLDQVENNPAGFDDDALAMRGSQKHGYHGVQNFGTFMRNAEVKASMGGFNPG
                     DACRGNMTETPEP"
     gene            complement(2043..3404)
                     /locus_tag="VN041_01960"
     CDS             complement(2043..3404)
                     /locus_tag="VN041_01960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU27844.1"
                     /translation="MERFDTVVVGAGMAGVTCARMLADAGRRVVVLEARDRVGGRMHT
                     DRAAGFPVDLGASWIHGIEQSPLWGLVQALGIPTREFTVGSFQAGGRPMEHFDGAGLP
                     MDAAASERWISDVDAVDAALVQQIAASARGDSYLVVTERALDASGLDAERIDEIREFF
                     RHRVEEQCGAWIGDLDAHGLDEDGIDGDEVIFPRGYDELPHRISSGLDLRTAHMVNAI
                     TRRSDGWAVRTIDGEFTAADVVVTVPLGVLKSGAIRFEPPLPAAAEGAIERLGMGVFN
                     KIFVQFPERFWRTDTYVIRALGEAGERWHSWYDVSSISGIPTLLTFAAGPLGRHLQTR
                     PDAEVVADSVKALRRLYGDAVGEPTASWVTRWGDDPFSAGSYSHLPIGASHHDHDALA
                     GPFEGGLHFAGEATWGAEPATVGAAYRSGHRAAERILGRSVDLAEFAMQVAERTVDHS
                     PRS"
     gene            3524..4501
                     /locus_tag="VN041_01965"
     CDS             3524..4501
                     /locus_tag="VN041_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU27845.1"
                     /translation="MTHSAEVSWQLDGIDMFGTFTTPDAGGPHPAVVMVAGSGPTDRD
                     WTSPLLPGDNGSAVLLAEYLAAAGIASLRYDKRASGPHVAENIPRIAGRMSMQSHLDE
                     LVAAVRVLAAQHNIDSARIVGLGNSEGCLHVLHYASSAQDLPFAGLVLTAPPGRSIGA
                     VLLTQLRTQIGSLPDGEELFPLVEQAAARYSAGEPMDADPRLPEGVRGVLASFETPVN
                     LPFARELWNEDATAALASVAVPTLVLIGRSDVQVDAELDGGPLQSAASGMPNVTFAFP
                     EHANHVLKEDLRSPEERAAAPGSGYNEPGTRLDPEALGAILSWLRDVVG"
     gene            4589..6649
                     /locus_tag="VN041_01970"
     CDS             4589..6649
                     /locus_tag="VN041_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HWU27846.1"
                     /translation="MTLAEPTRRNLPMLDHVRGKRSAVTCQLKCANACLGPECNTSAN
                     EDFRSIATAVFSRRALFGLGAAAAVAVAVGVGRGAGAPTSAPSAAGVGGPGASFARPG
                     RAGLSFGAIAPVPAEVDAFTVPDGYTWKPIIRWGDPLFSSTPAFDIEAQTPEAQAGQF
                     GYNSDYLDIVADPSGKTGVLVNNHEYVNPNLMFRDTDDAAELRRRGDIYKAAQGMSVV
                     EIRRRRGGDPWEYVVDGRRNRRITVETVFELTGPAAGSDLVKTAADPEGRWVHGTLGN
                     CSGGTTPWGTILSGEENFNGYFAWAADTAEQRRYSGSSTTSTSTGWEAYDPRFDAHQA
                     GYVNEPNRFGYIVEIDPQDPTSTPRKHTAMGRFKHEGANVRVAEDGRVVAYMGDDERN
                     DYLYKFVSKNRISGSRKKNLKLLSEGDLFVAKFRGDSPAAEITGTGALPTDGAFDGTG
                     EWLPLTRNGESVVPGFTTEQVLVYTRLAADAIGATKMDRPEDVEPSPVTGKVYVALTN
                     NSRRSEVDEANPVRGNRYGHVVEITETAGQSGTRFGWSILMLCGDPNTFENAYFAGFP
                     KELVSPISCPDNVAFDSQGDLWISTDGAPGTIGYGDGLFKVPLTGSERGHVQQFLSVP
                     RDAETCGPVIHDREGLVFVAVQHPGEDGTFDQPRSLFPDYGSTAQGDIATAPRPAVVQ
                     VHRG"
     gene            6730..7215
                     /locus_tag="VN041_01975"
     CDS             6730..7215
                     /locus_tag="VN041_01975"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HWU27847.1"
                     /translation="MDIWDLLGGLLDGVGVLIGVGALLLGVLAALAWLWGLRALLRRL
                     RESREIAGNWPRTAASIARVDMVPFPLPQDAPRPLAHYSFTVRDRAYAGEDAERLLAA
                     PAVGDTVEVVYDPADPAVNHLATRIRSRIGSWLALFIVMELALLGCVVLLFWLGIDAL
                     I"
     gene            7296..8285
                     /gene="nusA"
                     /locus_tag="VN041_01980"
     CDS             7296..8285
                     /gene="nusA"
                     /locus_tag="VN041_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWU27848.1"
                     /translation="MDIELGLLRGIEKEKAIPFDELVSIIEQAILTAYGKHVSADGAV
                     PEGVRVELNRTTGHVAVLQPVTDEEGAVIGEEEATPEDFGRIAAYAAKQVIGQRLRDI
                     ADDAVLGEFRGKEGDIVAGIIQQGPNPRMIHVDLGSVEAILPPEEQVPGEEYAHGTRL
                     RVYVTSVAKGNKGPQITVSRTHPGLVRKLFALEVPEIAAGLVEIVSLAREAGHRTKIA
                     VKANDPSVNAKGSCIGEMGRRVRAVTEELSGEKIDIVDHHPDLATFVANALSPAKVTS
                     AFVLDAGTKAVRALVPDYQLSLAIGKEGQNARLAAKLTGAKIDIQPDSVMAEE"
     gene            8355..8627
                     /locus_tag="VN041_01985"
     CDS             8355..8627
                     /locus_tag="VN041_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HWU27849.1"
                     /translation="MEPVRTCVGCRGRASRTALIRVALHNDELVFDERAVLPGRGAWL
                     HPSPECLDAALRRRAFARALRISPSIGFPEAADAQTTERYFHRNKG"
     gene            8722..>8975
                     /locus_tag="VN041_01990"
     CDS             8722..>8975
                     /locus_tag="VN041_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HWU27850.1"
                     /translation="MAGKPRVHEIAAELGVDSKFALAKLKELGEFVKSPSSTIEPPVA
                     RKLRAAIEADPSAKAADKPEAKPAAKAPASGAPKPGGPVPG"
BASE COUNT         1438 a         3177 c         3022 g         1338 t
ORIGIN      
        1 gtcacccgca tcctcgcgat catcgccgag ctgaacaacg acgacaaggg cctgatctgg
       61 ccggcatccg tcgccccgtt cgacgtgcag gtcgtcgccg cagggcgcga ccaggtcgcc
      121 ttcgacgtgg cggaggacat cgcgaaccag ctcgaggccg ctcgcctgga cgtgctctac
      181 gacgaccggg tgaaggtgtc gccgggcgtg aagttcggcg acgccgagct ggtaggcgtg
      241 ccgaagatcg tgatcgtggg ccgcggtgcg gcagacgggc aggtcgaact gtgggaccgc
      301 gcgaccggcg agcgggaggc cctctcggcg gcggatgccg tcgcgcgact cacgcgctga
      361 tccgacagca tccgtgcgag cacgggggac ggacgcaccc gtgaggagga tcatcgtgcc
      421 gtggcgcgat gatcctcctc gacgcgtcgc cgacctgctc gaggactccg ccgggctgcg
      481 cctgtgacag gctgagggca tgacggatgc cacgctgaac gacgacctgc gacacgaact
      541 gacgacgctt ctcgaccgtg cgggcatcca cgccgaccgt gggctgatcg cgcggatgct
      601 gcagaccgcc ctcaagctcg gtgtcgagca cacggaccgg ctcgacctga agatcgcgtc
      661 ggccgcgctg agcgagatgc acgatgcctt ccggctcttc cagcccttcc accaggtgcc
      721 caaggtcacg gtgttcgggt cggcgcgcac cgcgcaggac gatccgctct acatccaggc
      781 gcgggatgtg gccgccgcgc tcgcgagcga cgggtggatg gtcgtcacgg gcgccggccc
      841 cggcatcatg caggcagccg ccgagggagc cggcgcgaag atgtcgctgg gggtctccat
      901 ccggctgccg ttcgaggagc ggatgaacac gatcatcgcc caggacgcgc aggtcgtctc
      961 gatgaagtac ttcttcaccc gcaagctcat gctcatgaag gagtcccgcg ggttcatctg
     1021 cctgcccggc ggattcggca cgatggacga gatgttcgaa ctgctgacgc tgcagcagac
     1081 aggcaaggcg gagcccatgc cgatcgtgct gctcgacgcc cagggcggat cgttctggac
     1141 cggcctgaag accttcgtcg acgagcacct cgccccggca ggggtgatct cccgcgacga
     1201 cctcgaccgc gtgctcatca ccgactccgt cagcgaggcg gtggcgaaca tcaccggctt
     1261 ctggcgcaat tacgactcgc tgcgctgggt gggggacaga ctggtcctgc gcctggttca
     1321 cgaaccgacg gatgccgaca tcgacagcct caacgagcgc ttcggcggca tgtgcgcgga
     1381 agggcgcatc gagcgcaccg atccgctcga cgtggaactc gccgacgggg atgccgtcga
     1441 actgccccgc atcgtgctga acctcgcaca gcgctcggtg ggttcgctgt tcgcgctgat
     1501 ccgcgcgatc aacgagctcc cctcggcacg ctgacgcagg cggatcgaac gctcgccgcc
     1561 ggcagggccg acggcgagcg gtgcgcggga tcgactcagg gctccggggt ctcggtcatg
     1621 ttgccccggc aggcgtcgcc ggggttgaag ccgcccatcg aggccttgac ctcggcgttg
     1681 cgcatgaacg tgccgaagtt ctgcaccccg tggtaaccgt gcttctggct gccgcgcatg
     1741 gcgagcgcgt catcgtcgaa ccccgcaggg ttgttctcca cctggtccaa ggtgtcctgc
     1801 ccggagaacg cgcattcgga ggaggcgtga ttcgcgccct gcgcctcctt gccgttgccc
     1861 gtcacctctc ccgcgtacgc cgtgcccgcc ccgaccagca cgaacaccag cgcgacggtg
     1921 gcccccagtg ccattcgctt catgattccg ccttccatcg acggggcgca ccgatgcgac
     1981 ccgtgttccg acaaaaggct aggtgcgcgt catggcccgc gcatcagtgc tgatacggag
     2041 aatcatgacc ggggactgtg atccacggtc ctctccgcca cctgcatcgc gaactctgcg
     2101 aggtcgacgc tgcgcccgag gatccgctcc gccgcccgat gccccgaccg gtacgcggcc
     2161 ccgaccgtcg ccggctccgc gccccaggtc gcctcgcccg cgaagtgcag accgccttcg
     2221 aacggccccg ccagtgcgtc gtgatcatga tgcgacgcgc cgatcggcag atgcgagtag
     2281 gagccggccg agaaggggtc gtcgccccac cgggtcaccc acgaggcggt cggctcgccc
     2341 acggcatccc cgtacaggcg gcggagcgcc ttgacggagt ccgcgacgac ctcggcatcc
     2401 ggacgggtct gcagatgcct gcccagcgga ccggccgcga aggtgagcag agtcgggatg
     2461 ccgctgatcg acgacacgtc gtaccaggag tgccagcgct cgcccgcctc gccgagcgcg
     2521 cggatgacgt acgtgtccgt gcgccagaag cgctcgggga actgcacgaa gatcttgttg
     2581 aacaccccca tgcccaggcg ctcgatggct ccctccgcag ccgccggcag cggcggctcg
     2641 aagcgaatcg caccagactt cagcacgccg agcggaaccg tcacgaccac gtccgccgcg
     2701 gtgaactcgc cgtcgatcgt gcgcacggcc cagccgtcgc tcctgcgggt gatcgcgttg
     2761 accatatgcg ccgtgcgcag gtccaacccc gacgagatgc ggtgcggcag ctcgtcatag
     2821 ccccgcggga agatcacctc atcgccgtcg atgccgtcct cgtcgagacc gtgcgcgtcg
     2881 aggtctccga tccaggcacc gcactgctcc tcgacacgat ggcggaagaa ctcgcggatc
     2941 tcgtcgatcc gctcggcgtc cagcccggag gcgtccagcg cgcgttcggt cacgaccagg
     3001 tagctgtccc cgcgggcgga ggcggcgatc tgctgcacga gcgcggcgtc caccgcgtcc
     3061 acatcggaga tccatcgttc ggatgccgca gcatccatcg gcagtccggc gccgtcgaag
     3121 tgctccatcg gcctaccgcc cgcctggaag ctccccacgg tgaactcccg cgtcggtatg
     3181 ccgagcgcct gaaccagccc ccagagcggc gattgctcga tgccgtggat ccaggaggcg
     3241 ccgaggtcga cggggaaacc ggcggcgcgg tccgtgtgca tccgccctcc gacgcggtcc
     3301 ctcgcctcca gcaccacgac gcgcctgccg gcgtcggcca gcattctggc gcaggtgaca
     3361 cccgccatcc ccgcaccgac gacgaccgtg tcgaatcgct ccatgccctc agtatggagc
     3421 gtcctggccc ggcgacccgg cagggtccgc gctcgccgcc gtgcgcatgc cgagcgccga
     3481 atggcactcc cgagggcgat cgccggagtg tcatcatgag cacatgacgc attcagcgga
     3541 ggtcagctgg cagctcgacg gcatcgacat gttcggcacg ttcaccacgc cggatgccgg
     3601 tggaccgcat cccgcagtcg tgatggtcgc aggcagcgga cccacggatc gcgactggac
     3661 ctcgccactg ctgccggggg acaacggctc cgcggtgctg ctggccgagt acctcgccgc
     3721 ggcaggcatc gcctcgctgc ggtacgacaa gcgcgcctcc gggccgcacg tcgcggagaa
     3781 catcccgagg atcgccggcc ggatgagcat gcagtcccac ctcgacgagc tcgtggcggc
     3841 ggtccgcgtc ctcgccgccc agcacaacat cgattccgcc cgcatcgtcg gtctcggcaa
     3901 cagcgagggg tgcctgcacg tgctgcacta cgcgagcagc gctcaggacc tgcccttcgc
     3961 cggactcgtg ctcacagcgc ctcccggccg cagcatcgga gcggtgctgc tgacccagtt
     4021 gcgcacgcag atcggatccc ttcccgacgg cgaggagttg tttccgctcg tcgagcaggc
     4081 cgccgcgcgt tacagtgccg gcgagccgat ggacgccgat ccgcgcctgc cggagggggt
     4141 gcgcggcgtg ctggcgagct tcgagacccc ggtgaacctc ccgttcgcgc gcgagctgtg
     4201 gaacgaggat gcgacggcgg ccctcgcgtc ggtcgccgtc cccactctgg tgctgatcgg
     4261 acgcagcgac gttcaggtgg acgccgagct cgacggcggt ccgctccagt cagcggcatc
     4321 cgggatgccg aacgtgacct tcgcgttccc cgagcacgcc aaccatgtcc tcaaggagga
     4381 tctgcgctcg ccggaggagc gggccgccgc tccggggagc gggtacaacg agccgggcac
     4441 gcgcctggac cccgaggcgt tgggcgccat cctctcctgg ctgcgcgacg tggtgggctg
     4501 acgcgcaacg ccgagttcaa acgccgttca tccgtccgtc atcagcaccc ggcatcgtgt
     4561 catccaccgc atccgacgag gaggaccgat gactctggcc gaacccacgc gacgcaatct
     4621 gcccatgctc gatcacgtgc gaggcaagcg ctcggcggtc acctgccagc tcaagtgcgc
     4681 gaacgcgtgc ctgggtcccg agtgcaacac gtccgcgaac gaggacttcc gttcgatcgc
     4741 gacggccgtc ttctcgcgtc gggcgctctt cggactcggt gctgcggccg cggtggcggt
     4801 ggctgtgggg gtgggacgag gcgcgggcgc accgacttcc gcgccctcgg cggcaggtgt
     4861 cgggggaccg ggtgcatctt tcgcgcggcc tggtcgcgca ggcctgtcat tcggtgcgat
     4921 cgctccggtg ccggccgagg tggatgcctt caccgtgccg gacggctaca cctggaagcc
     4981 gatcatccgc tggggcgacc cgctcttctc gagcactccg gcgttcgaca tcgaggcgca
     5041 gacgcccgag gcgcaggccg ggcagttcgg ctacaacagc gactacctcg acatcgtcgc
     5101 ggatcccagc ggcaagaccg gcgtgcttgt gaacaaccac gagtacgtca acccgaacct
     5161 catgttccgt gacaccgacg atgccgccga gctgcgtcgt cgcggtgaca tctacaaggc
     5221 cgcccagggc atgtcggtcg tggagatccg tcgtcgccgc gggggggacc cgtgggagta
     5281 cgtcgtcgac ggccgccgga accgccgcat caccgtcgag acggtgttcg agctcaccgg
     5341 tcccgcggcc gggtccgacc tcgtcaagac cgccgccgac ccggagggcc gctgggtgca
     5401 cggcactctg ggcaactgct ccggaggcac cactccctgg ggcaccatcc tctccggcga
     5461 ggagaacttc aacggctact tcgcgtgggc ggccgacacg gccgagcaga ggcggtacag
     5521 cggctcgagc acgacgagca cgtccaccgg ctgggaggcc tacgacccgc gcttcgacgc
     5581 gcaccaggcg ggctacgtga acgagccgaa ccggttcggc tacatcgtcg agatcgaccc
     5641 gcaggacccc acctccacgc cgcggaagca caccgcgatg gggcgcttca agcacgaggg
     5701 cgccaacgtg cgcgtcgccg aggacggtcg tgtcgtggca tacatgggcg acgacgagcg
     5761 caacgactac ctctacaagt tcgtctcgaa gaaccgcatc tcgggttcgc gtaagaagaa
     5821 cctgaagctg ctcagcgagg gcgacctgtt cgtcgccaag ttccgcggcg actcgcccgc
     5881 cgcggagatc accggaaccg gcgcgctgcc gaccgacgga gcgttcgacg gtaccggaga
     5941 gtggcttccg ctcaccagga acggcgagag cgtcgtgccg ggcttcacca ccgagcaggt
     6001 gctcgtctac acccgcctcg ccgccgacgc gatcggcgcg acgaagatgg atcgccccga
     6061 ggacgtcgag ccgagcccgg tcaccggcaa ggtgtacgtc gcgctcacca acaactcccg
     6121 gcgcagcgag gtcgacgagg ccaacccggt tcgcggcaac cgctacggac acgtggtgga
     6181 gatcaccgag acggccggac aatcgggaac ccgcttcggg tggagcatcc tgatgctgtg
     6241 cggcgatccg aacaccttcg agaacgccta cttcgccgga ttcccgaaag agctggtctc
     6301 tccgatctcc tgccccgaca acgtcgcgtt cgactcccag ggcgacctgt ggatctccac
     6361 ggacggcgcg ccggggacga tcggctacgg cgacgggctg ttcaaggtgc cgctgaccgg
     6421 atccgagcgc ggccacgtgc agcagttcct ctccgtgccg cgggacgccg agacctgcgg
     6481 tccggtgatc cacgaccgcg agggtctcgt gttcgtcgcg gtgcagcatc cgggggagga
     6541 cggcaccttc gaccagccgc gctcgctgtt cccggactac ggctcgaccg cgcagggtga
     6601 catcgcgacg gcgccgcggc ccgccgtcgt gcaggtgcac cggggctgag cggatgccgg
     6661 tgctgcgagc tccgggcgcg cggcaccggc actgacatca ggcccgccgt atcgggctag
     6721 ccttccaggg tggacatctg ggacctgctg ggcggcctgc tggacggagt cggcgtgctg
     6781 atcggcgtcg gcgctctgct gctcggtgtg ctcgccgcgc tcgcgtggct ctggggcctg
     6841 cgcgccctgc tgcgcaggct gcgcgagtcg cgggagatcg ccgggaactg gccgcgcacc
     6901 gccgcgtcga tcgcccgggt cgacatggtc ccctttccgc tgccgcagga tgcccctcgg
     6961 ccgctggcac actacagctt caccgtccgc gatcgagcgt acgcaggcga ggacgccgag
     7021 cggcttctgg cggcacctgc ggtcggcgac actgtggagg tcgtctacga tcccgccgac
     7081 cctgccgtca accacctcgc gactcgcatc cggtcccgga tcgggtcgtg gctggcgctg
     7141 ttcatcgtga tggagctcgc tctgctcggg tgcgtcgtgc tgctcttctg gctcggcatc
     7201 gacgcgctca tctgagtgac ccgaggttcg ggtattcttt tctgatgccc gcggagcgtc
     7261 tgtccgcgga gagagctgaa tagaggagac cgctgatgga catcgaactc ggactgctgc
     7321 gcggaatcga gaaggagaag gcgatcccct tcgacgagct cgtctcgatc atcgagcagg
     7381 ccatcctcac cgcatacggc aagcacgtct ccgctgacgg cgccgtgccc gagggcgtgc
     7441 gcgtcgaact gaaccgcacc accggccacg tcgccgtgct gcagcccgtc accgacgagg
     7501 agggcgcggt catcggcgaa gaggaggcga ctcccgagga cttcggtcgc atcgctgcct
     7561 acgcagccaa gcaggtcatc ggccagcgcc tgcgcgacat cgccgacgac gccgttctcg
     7621 gcgagttccg tggcaaggag ggcgacatcg tcgccggcat catccagcag ggccccaacc
     7681 cgcggatgat ccacgtcgac ctcggctccg tcgaggcgat cctcccgccc gaggagcagg
     7741 tgcccggcga ggagtacgcc cacggcaccc gcctgcgcgt gtacgtgacc agcgtcgcca
     7801 aggggaacaa gggcccgcag atcaccgtgt cgcgcacgca ccccggcctg gtccgcaagc
     7861 tgttcgccct cgaggtgcct gagatcgcgg ccggtctggt cgagatcgtc tcgctcgccc
     7921 gggaggcagg acaccgcacc aagatcgccg tcaaggccaa cgacccctcg gtcaacgcca
     7981 agggctcctg catcggcgag atgggccgcc gcgtgcgggc cgtcaccgag gagctctccg
     8041 gtgagaagat cgacatcgtc gatcaccacc cggatcttgc gaccttcgtc gccaatgcgc
     8101 tctcgcccgc caaggtgacc tcggcgttcg tgctcgatgc cggcaccaag gccgtgcgcg
     8161 cactcgtccc ggactaccag ctctcgctcg cgatcggcaa ggaggggcag aacgcccgcc
     8221 tggccgccaa gctgaccggc gccaagatcg acatccagcc cgacagcgtg atggcggagg
     8281 agtgatcgcg ggggagcgac gcactctgcg cacacgccgg gcgccgggct tcgatccgcc
     8341 tcacgaggtg taagatggaa cctgtacgaa cgtgtgtcgg ctgccgcggt cgtgcctctc
     8401 gaaccgccct gatcagggtg gccctgcaca atgacgagct cgtcttcgac gagcgagccg
     8461 tgctgccggg gaggggcgcg tggctgcatc cgtcacccga gtgcctggat gccgcgctgc
     8521 ggcgccgagc cttcgcacga gcacttcgca tctctccgtc catcggtttc cccgaagcgg
     8581 cggatgcgca gaccaccgaa cggtacttcc accgaaacaa aggctgaacg gctatggaaa
     8641 caaagtgaac ggctcgaaat gagacccgtc cgcgactagt ggtctgccct gccagggcgg
     8701 accgaccctg acaggagaat tgtggctggt aaaccacgcg tgcacgagat cgccgctgaa
     8761 ctcggcgtcg acagcaagtt cgctctcgcg aagctcaagg agctcggcga gttcgtgaag
     8821 agtccctctt ccaccatcga gccccctgtg gctcgcaagc tccgcgctgc catcgaggcc
     8881 gacccgtcgg ccaaggccgc ggacaagccc gaggcgaagc cggcggcaaa ggcaccggca
     8941 tccggcgcgc ccaagcccgg tggtccggtc cccgg
//
LOCUS       DATMSX010000066        10486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1009,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000066 DATMSX010000000
VERSION     DATMSX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1555)
                     /gene="lnt"
                     /locus_tag="VN041_01995"
     CDS             complement(29..1555)
                     /gene="lnt"
                     /locus_tag="VN041_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWU27851.1"
                     /translation="MGRTRPAASVRPLVPLWLAVWLAAIAGVLMSISFPSLAIWPVAL
                     VAVALLIVCLDGRRLRSALLVTGVYGLVFYSMLVGWVARYLGPVPWVALAVVEGVLTA
                     VALLPIALAYRWMPHAWPRSRLILLPAIVAALWTVQELFIGNWPYGGFPWARLGMTQA
                     EGPFAPVSSWVGVSGLSFLMVFAVALIVEVVRARAWRRPLALVTPAVTALVLLFLPVF
                     PTAHAGTMRIAAVQGNGPTGYFDTREQYSVIDAQMAATEPLEGEKADLIVWPEGGVDY
                     DPFQDASTARRLNRVVSWLDAPLLANAATERGKDIFNTSFLWTDEGTAAQLHDKRHPV
                     PFGEYVPNREFFNALAPDLIGLLQREYTPGTNPPLVKVGDVGVGLAICFDVIYDEVIQ
                     EGVQRGAQVLVFQTNNADFRGTDENLQQLAFARMRAIETGRSVVNISTVGTSQIMRPD
                     GSTVATLDADEAGALLEDVELRSGLTAGVLLGPWIQLALVIGGSGALVAAGVIARRRR
                     "
     gene            complement(1558..4032)
                     /locus_tag="VN041_02000"
     CDS             complement(1558..4032)
                     /locus_tag="VN041_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU27852.1"
                     /translation="MSSPAERYASAQTAAQHPQTRQFAAAQRFALDPFQLDACQALER
                     GRSVLVAAPTGAGKTIVGEFAIHLAMQTPGDKAFYTTPMKALSNQKFRELVDVYGADE
                     VGLLTGDTNINGNARIVVMTTEVLRNMLYAGSDSLRGLRYVVMDEVHYLADRFRGPVW
                     EEVILHLPQEVRLISLSATVSNAEEFGDWLDTVRGDTDVIVSEIRPVPLEQHVLVRDD
                     LLPLFDDRAGVATAQVNQELFRIRSFTGSRYESNRSAQEYASNRHAGRQAKRPPRGGK
                     RPVRSANMRRIERMDRPDVVRLLERSNLLPAIFFIFSRVGCDAAVQQVRRAGIRLTTQ
                     EERTEIREIVNERTRSLQDEDLDVLGFWEWRDDLERGVASHHAGLLPAFKEVVEDLFQ
                     RKLVKAVFATETLALGINMPARTVVLEKMEKFNGEARVAITSGEYTQLTGRAGRRGID
                     VEGHAVVQWTEGMDPQAVAALASRRTYPLNSSFRPTYNMAVNLIDRFGRSRAREILES
                     SFAQFQADRAVVGLARKVKDAEESLAGYQKAMQCEHGDFLEYAAIRRELSDLEKKNRR
                     DAAGPRVAREKRMRKIQALRTRMQRHACHGCPDREAHARWAERYFSLKRDTDRIRRQI
                     ETRTGTVARTFDRVIDVLRVVDYVEGDGDDLLLTDAGRTMKRIYGERDLLVAEALRQG
                     LWRSLDAPSLAAMASCLVYEPRRDEQNAGERGLPRGAFRIAYDATVRLWAELDDIEHD
                     YSLPRTEPLAPGLAAAMHSWARGGSLDRVLIDADMAAGDFVRWAKQTIDLLDQLSIVA
                     DDGDLASTARRALDGVRRGIVAYAGV"
     gene            complement(4029..4793)
                     /gene="tatC"
                     /locus_tag="VN041_02005"
     CDS             complement(4029..4793)
                     /gene="tatC"
                     /locus_tag="VN041_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWU27853.1"
                     /translation="MSLGEHLREARRRLIIAVIALVIGMVIAAILTEPVLKLMAWPIN
                     EINALRGKDFAALMFTKISSGFNLRLRMSLAIGLLLSAPIWLWQIWAFIVPGLTKKEI
                     KYTVGFVVAAIPLFFAGGAVAIWVAPHVIEVMSGFVPGIGKNFLDAEYYYDFILKFIL
                     VIGISFVLPVFMVALNLAGVITGRGLLKGWRIAVLVASVFAAIATPAADIGSMFLVGG
                     ILIVLYFAAAGVSLLFDRRKRKKRIADGLDPDAAAA"
     gene            complement(4851..5108)
                     /gene="tatA"
                     /locus_tag="VN041_02010"
     CDS             complement(4851..5108)
                     /gene="tatA"
                     /locus_tag="VN041_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWU27854.1"
                     /translation="MGNLFAGPHLWIILIIILLLFGAAKLPALAKSLGQSARVFKGEM
                     KAMKDDDAPEAGTDGTTATSTGTAAPAAERPADQDTPPRAS"
     gene            complement(5140..6129)
                     /locus_tag="VN041_02015"
     CDS             complement(5140..6129)
                     /locus_tag="VN041_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWU27855.1"
                     /translation="MSSAPLLAADRVRLYLTLVPYLLERGQVTLDEAAAEFGVTAAQM
                     RAMVEKLTVIGLPGDSGYWQLPQEMFDIDWDLLDEHDIIEITHDVAFRRVPRFTAREA
                     AALLAGLQLVAAVPAVADSGLVSGLIAKLSRGSADAPPDLLIAPVEVTEVQQVVSRAL
                     HDGVAVAFRYQAPDAEPTTRTVDPRQILITNGQWYLQGWCHLRQAMRTFHLDRVSDAH
                     LTEIPITHGDAPVPAEFGGGGDVSDHVTVRLPERMLPLLGAFPYEEIDRADAVVTVRM
                     SMADPRGIKRIAGRFGGALEVLDPPLARCATREWASAALELYTVPDGSSAQVD"
     gene            complement(6126..7136)
                     /locus_tag="VN041_02020"
     CDS             complement(6126..7136)
                     /locus_tag="VN041_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWU27856.1"
                     /translation="MTPAQIPAEERLTNLVVALMATEIGLTKQQILDNVSGYRQRVEA
                     GAKPDSLEKMFERDKDELRALGMPVETIGDPADPQDMREARYRIPQADYDLPSDIEFS
                     DAELAVLRLAGSVWSSESVSADAQAGVRKIRALGIDGDEPIIGFAPRIAVQDAAFPAL
                     QEGIERNRVVVFDYLKPGEDAPTRRRIRPLVQVEYESRWHLYGYDVDADGERTFLLSR
                     IVSDVKVTGQGFDPVARDGAAERALAGLAEVAASQQALLEITPGTEAALRLGRRGVPA
                     VQGIRVPYVDRHIFADELATYGPEVRVVEPADLRDSVIQRLHAIVDAHAADAIAGEGS
                     AE"
     gene            complement(7133..8044)
                     /locus_tag="VN041_02025"
     CDS             complement(7133..8044)
                     /locus_tag="VN041_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU27857.1"
                     /translation="MRKTTAAVSALALSALVLTGCSTAPGSGAAGCERSESAALSMAV
                     KASGDIGAAKVSLTSPMPTSKVVYDDLVVGDGAVVRDETQDVVGSITLLNGATGQMID
                     SGAGVWSPKSLSEQFGGIGSALQCATNGSRVAFAVPAKDLPEGMAAQVGLGPDESIVG
                     TIDIQEVLLPKAQGRDVFNDAQGIPSVVRAPSGQPGIIIPDGAAPKKLVTETLIEGDG
                     AKVGKGLAMFQYTAVKWSTRAVTGSSWEKGVAYDTSTLPPQVMAQVAKATVGSQLLVV
                     DPEKTGDDAIAYVVDVLGIVPPELVRG"
     gene            complement(8055..9065)
                     /locus_tag="VN041_02030"
     CDS             complement(8055..9065)
                     /locus_tag="VN041_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HWU27858.1"
                     /translation="MSANRPRGPFREGDRVQLTGPKGRMHTITLRGDGELHTHHGVLK
                     HTQLIGEPDGSVVTNSGGHDYLALRPLLRDFAMSMPRGAAIVYPKDSAQIVMQADIFP
                     GAVVVEAGVGSGALSLALLRAIGHEGRLTSFERREDFADVARANVETFFGETPDTWNV
                     VVGDLAEELPAQFEAGTVDRVVLDMLAPWECMDVVADALAPGGVVICYVATATQLSRV
                     AEYIRGTGLFTDPDASETMVRGWHVEGLAVRPDHRMVAHTGFLLTARRLAPGAVPPEV
                     KRRASKSSYGDEDVELWTPGAVGDREITDKNLRKRVREAQKAANGARTAAASREGDSA
                     SE"
     gene            complement(9062..9745)
                     /locus_tag="VN041_02035"
     CDS             complement(9062..9745)
                     /locus_tag="VN041_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWU27859.1"
                     /translation="MGSVSNKPSAVLWDMDGTLVDTEPYWMDAETVLVESFGGTWTHE
                     DALQLVGNGLHDSALILQKYGVDMEADAIVQHLTDAVAHRLRSEGVPFRHGARELLAD
                     LRANGIPTALVTMSLRRMALDVVSLIDFDAFDLVVAGDDVARPKPFPDPYLRAAELLE
                     IDIADAVVIEDSGTGVAAGRASGAVTLGVPHIVSLEGSDAHELWPTLAGRTSADIRDL
                     AGRHAKENA"
     gene            9838..>10486
                     /locus_tag="VN041_02040"
     CDS             9838..>10486
                     /locus_tag="VN041_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWU27860.1"
                     /translation="MDALGSRIIVAAFDGWNDAGEAASGAIAALREAGSYEAVHSVDP
                     ELYFDYQYTRPSSRMDASGRRELHWPDATLWRPTSTTEGPELWLLTGSEPARAWQAFA
                     TEFIDVALRDDVTGLVTLGAMLSDVPHTRPISIFASSQNERVRAAHGLDKSTYEGPVG
                     ILSVFEHFAERAEIPSASLWASVPHYVATAAPSPKATLALLDRLEELTGVGPAREH"
BASE COUNT         1628 a         3648 c         3621 g         1589 t
ORIGIN      
        1 ccgacgcgcg agcgcatcgg cgcctgattc agcgtcgccg ccgcgcgatc acgccggcgg
       61 cgaccagtgc ccctgagccc ccgatcacca gcgcgagctg gatccacggg cccagcagca
      121 caccagccgt gaggccggaa cgcagttcga catcctcgag cagcgctccg gcctcatctg
      181 cgtccagggt cgcgacggtg ctgccgtccg gccgcatgat ctggctcgtg ccgacggtcg
      241 agatgttgac cacgctgcga ccggtctcga tcgcccgcat ccgcgcgaac gccagctgct
      301 gcaggttctc gtccgtgccg cggaagtcgg cgttgttggt ctggaagacg agcacctggg
      361 cgccgcgctg cacgccctcc tggatcacct cgtcgtagat cacgtcgaag cagatcgcga
      421 gccctacgcc gacgtcgccg accttcacca gcggagggtt cgtccctggc gtgtactccc
      481 gctgcaggag accgatcaga tccggggcga gcgcattgaa gaactcgcga ttcggcacgt
      541 actcgccgaa cggcaccggg tgccgcttgt cgtgcaactg agccgcagtg ccctcgtccg
      601 tccacaggaa cgaggtgttg aagatgtcct tgccgcgttc tgtggccgcg ttggccagca
      661 ggggcgcgtc cagccacgac acgacgcggt tgagccgtcg agcagtggag gcgtcctgga
      721 acggatcgta gtcgacgccg ccctcgggcc acacgatcag gtcggccttc tcaccctcca
      781 gcggctcggt cgccgccatc tgcgcgtcga tgacgctgta ctgctccctg gtgtcgaagt
      841 agccggtcgg tccgttgccc tgcacggccg cgatgcgcat ggtccccgcg tgcgcggtcg
      901 ggaacacggg caggaacagc agcaccaggg cggtgacggc cggcgtcacg agcgcgagag
      961 gacgccgcca ggcgcgggcg cggacgacct cgacgatcag cgccacagcg aagaccatga
     1021 ggaagctcag gccactcacg cccacccagg acgagacggg cgcgaagggc ccttcggcct
     1081 gcgtcatgcc cagccgggcc caggggaagc cgccgtaggg ccagttgccg atgaagagct
     1141 cctgcacggt ccacagcgcc gccacgatcg cgggaagcag gatcagacgg ctgcgcggcc
     1201 aggcatgcgg catccagcgg tacgcgagcg cgatcggcag cagcgcgacg gccgtcagta
     1261 cgccctccac gacggccagg gcgacccacg gcaccggccc gagataccgt gcgacccagc
     1321 cgaccagcat cgagtagaag acgaggccgt acacgccggt gacgaggagc gcggaccgga
     1381 ggcggcgccc gtcgaggcag acgatcagga gcgcgaccgc gaccagcgcg acgggccaga
     1441 tcgccagcga ggggaaggag atgctcatca gcacgccggc gatcgcggcc agccagaccg
     1501 cgagccacag cggcacgagc gggcgcacgg atgccgcggg gcgagtcctt cccaccgtca
     1561 cactcccgcg taggcgacga tgccgcgacg caccccgtcc agggcgcgcc gtgcggtgga
     1621 cgcgagatcg ccgtcgtcgg cgacgatcga gagctggtcc agcaggtcga tggtctgctt
     1681 cgcccagcgc acgaagtctc ccgccgccat gtcggcgtcg atcagcacgc ggtccagcga
     1741 gcccccgcgc gcccaggagt gcatcgcggc cgccaggccc ggcgcgagcg gctcggtgcg
     1801 cgggagggag tagtcgtgct cgatgtcgtc gagctcggcc cacagcctca cggtggcgtc
     1861 gtaggcgatg cggaacgcgc cgcgaggcag accccgctcc cccgcgttct gctcgtcgcg
     1921 tcgaggttcg tagaccaggc agctcgccat cgccgcgagc gaaggcgcgt ccaggctgcg
     1981 ccagaggccc tgccggagcg cctcggccac cagcaggtcc cgctcgccgt agatccgctt
     2041 catggtgcgg ccggcatcgg tcagcaagag gtcgtcgccg tcgccctcca cgtagtccac
     2101 gacccgaagc acgtcgatga cgcggtcgaa ggtgcgcgcg accgtgccag tgcgggtctc
     2161 gatctggcgg cggatgcggt cggtgtcgcg cttcaggctg aagtagcgct ccgcccagcg
     2221 ggcgtgcgcc tcgcggtcgg ggcatccgtg gcacgcgtgc cgctgcatgc gggtgcggag
     2281 cgcctggatc ttgcgcatcc gcttctcccg cgcgacgcgg ggaccggcgg cgtcacggcg
     2341 gttcttcttc tcgaggtcgc tgagctcacg ccggatggcc gcatattcca ggaagtcgcc
     2401 gtgctcgcac tgcatcgcct tctgatagcc cgccagcgac tcctcggcat ccttcacctt
     2461 gcgggccagc ccgaccactg ctcggtccgc ctggaactgg gcgaatgacg attccaggat
     2521 ctcgcgggcg cgcgagcgcc cgaaccggtc gatgaggttc accgccatgt tgtaggtggg
     2581 ccggaagctg gagttgagcg gatacgtgcg gcgggatgcg agagcggcga cggcctgcgg
     2641 atccatgccc tccgtccact gcacgacggc atggccctcg acgtcgatcc cgcgacgccc
     2701 cgcgcggccg gtcagctgcg tgtactcgcc ggaggtgatg gcgacgcggg cctcgccgtt
     2761 gaacttctcc atcttctcga gcacgacggt gcgggcgggc atgttgatgc ccagagccag
     2821 agtctcggtg gcgaagaccg ccttcaccag cttgcgctgg aacaggtcct cgacgacctc
     2881 cttgaacgcc ggaaggaggc cggcgtggtg cgacgcgacg ccgcgctcga ggtcgtcgcg
     2941 ccattcccag aagcccagga cgtcgagatc ctcgtcctga agcgatcgcg tgcgctcgtt
     3001 gacgatctcg cggatctcgg tgcgctcctc ctgcgtggtg aggcggatgc ccgctcggcg
     3061 cacctgctgc acggcggcgt cgcagccgac cctgctgaag atgaagaaga tggccggcaa
     3121 caggttcgag cgctccagca accgcacgac gtccggacgg tccatccgct cgatgcgccg
     3181 catgttggcg gagcgcacgg gcctcttccc gccccgcggg ggacgtttcg cctgccgtcc
     3241 ggcatggcgg ttgctggcgt actcctgggc gctgcggttg ctctcgtacc gggacccggt
     3301 gaacgagcgg atgcggaaca gctcctggtt cacctgcgcg gtcgccacgc ccgcgcggtc
     3361 gtcgaacagc ggcagcaggt catcgcggac cagcacgtgc tgctccagcg gcacgggccg
     3421 gatctcggag acgatcacgt cggtgtctcc gcgcacggtg tcgagccagt cgccgaactc
     3481 ctcggcgttg gagaccgtcg cactgagcga gatcaggcgc acctcctgcg gcagatgcag
     3541 gatgacctcc tcccagaccg gtccacggaa tcggtcggcg aggtagtgca cctcgtccat
     3601 caccacgtag cgcaggcctc ggagcgagtc cgagccggcg tacagcatgt tgcgcaggac
     3661 ctcagtggtc atcaccacga tgcgcgcgtt gccgttgatg ttggtgtcgc cggtgagcag
     3721 ccccacctcg tcggcaccgt agacgtcgac gagttcgcgg aacttctggt tggagagcgc
     3781 cttcatcggc gtcgtgtaga acgccttgtc gcctggtgtc tgcatcgcga ggtggatcgc
     3841 gaactcgccg acgatagtct tgcccgctcc cgtgggtgcg gcgaccagca cgctcctgcc
     3901 gcgctcgagc gcctggcagg cgtcgagctg gaacggatcc agggcgaagc gctgtgcggc
     3961 agcgaactgc cgcgtctgag gatgctgcgc cgccgtctgt gcggatgcgt agcgctcagc
     4021 cggcgagctc atgcggcggc tgcgtccgga tcgagaccgt ccgcgatccg cttcttccgc
     4081 ttacgacggt cgaagagaag cgacactccg gccgcggcga agtacagcac gatcaggatg
     4141 ccgccgacca ggaacatcga tccgatgtcg gccgccggcg tcgcgatggc ggcgaagacg
     4201 gaggcgacga gcaccgcgat ccgccatccc ttcagcagtc ccctgcccgt gatcacaccc
     4261 gcgaggttca gcgccaccat gaacaccggc agcacgaacg agatgccgat gaccaggatg
     4321 aacttcagga tgaagtcgta gtagtactcg gcgtcgagga agttcttgcc gataccgggc
     4381 acgaagcccg acatcacctc gatcacgtgc ggcgccaccc agatcgcgac cgcacctccc
     4441 gcgaagaaga gcggaatcgc cgcgacgacg aatccgaccg tgtacttgat ctccttcttg
     4501 gtgagtccgg gcacgatgaa cgcccagatc tgccagagcc agatcggtgc ggacagcagc
     4561 agaccgatgg cgagggacat ccgcaggcga agattgaaac cggacgagat cttcgtgaac
     4621 atcagcgcgg cgaagtcctt gccacgcagc gcgttgatct cgttgatcgg ccacgccata
     4681 agcttgagca ccggttcggt gaggatcgcc gcgatcacca tgccgatgac cagcgcgatc
     4741 accgcgatga tcaggcgccg acgagcctcc ctgaggtgct cgcccagcga catccgtcga
     4801 tcgtggctcc cctcggaaga aggcgcgtcg acgctcagtt ctgtcacgac ctacgaggcg
     4861 cggggcggtg tgtcctgatc agcaggacgc tcagccgccg gggctgcagt accggtcgac
     4921 gtcgcggtgg tgccgtcggt gccggcctcc ggggcgtcgt cgtccttcat cgccttcatc
     4981 tcgcccttga acacgcgtgc agactgtccc aggctcttcg ccagcgccgg cagcttcgcc
     5041 gcgccgaaca gaagaaggat gatgatcaga atgatccaca ggtgcgggcc tgcgaaaaga
     5101 ttacccatgg atttctcctc ttggtgggtc ggattccgtt cagtctacct gtgccgaaga
     5161 gccgtcgggc accgtataga gctcgagtgc ggcggatgcc cattcccgtg tcgcgcaccg
     5221 tgccagaggc gggtcgagca cctccagcgc acccccgaat cgaccggcga tccgcttgat
     5281 cccccggggg tccgccatgc tcatgcgcac ggtgacgacg gcgtcggccc ggtcgatctc
     5341 ctcgtacggg aaggcgccca gcagtggcag catccgctcg ggaagccgca cggtgacgtg
     5401 gtccgagacg tcgccgcccc cgccgaactc cgccggcacg ggcgcgtcgc cgtgggtgat
     5461 cgggatctcg gtcagatgcg catcgctgac gcggtcgaga tggaacgtcc gcatcgcctg
     5521 ccgcagatgg caccatccct gcagatacca ctgtccgttc gtgatcagga tctgacgcgg
     5581 gtcgaccgtg cgggtggtcg gctcggcatc cggagcctgg taccggaacg ccacggcgac
     5641 gccgtcgtgc agcgcgcgcg agacgacctg ctgcacctcg gtgacctcca ccggcgcgat
     5701 gagcagatcg ggaggagcgt cggcggagcc ccgggagagc ttggcgatca ggccgctgac
     5761 cagtccggag tcggccaccg caggcaccgc ggcgacgagc tggagaccgg cgagcagcgc
     5821 tgccgcctcc cgcgcggtga accgaggaac gcgacggaag gcgacgtcat gcgtgatctc
     5881 gatgatgtcg tgctcgtcga gcaggtccca gtcgatgtcg aacatctcct gcgggagctg
     5941 ccagtagccg gaatcgcccg gaaggccgat caccgtgagc ttctcgacca tggcccgcat
     6001 ctgcgcggcg gtgacgccga actcggccgc ggcctcgtcg agcgtgacct ggccgcgctc
     6061 gaggaggtac gggacgagcg tcaggtacag gcgtacgcgg tccgcagcca gaagcggtgc
     6121 ggaactcatt cggcactccc ctctccggcg atcgcgtcgg cggcgtgcgc gtcgacgatc
     6181 gcgtgcagcc gctggatcac gctgtcgcgg agatctgcgg gctccacgac ccgcacctcc
     6241 ggtccgtagg tggccagttc gtcggcgaag atgtgccggt ccacatacgg gacgcggatg
     6301 ccctgcactg cgggaacgcc ccggcggccg agccgcaggg ccgcctcggt ccccggcgtg
     6361 atctcgagca gggcctgctg cgatgcggcg acctcggcaa gacccgcgag cgcacgctcc
     6421 gcagcgccgt cgcgcgccac ggggtcgaag ccctgaccgg tgaccttcac atcgctgacg
     6481 atgcggctga gcaggaaggt gcgctcaccg tcggcgtcca cgtcgtaccc gtacaggtgc
     6541 cagcgcgact cgtactcgac ctggaccagc ggacggatgc ggcgacgcgt gggcgcgtcc
     6601 tcgccgggct tcaggtagtc gaagacgacg acgcggttgc gttcgatgcc ctcctgcagt
     6661 gcggggaagg ccgcgtcctg cacggcgatg cgcggcgcga agccgatgat cggctcgtct
     6721 ccgtcgatgc cgagcgcgcg gatcttgcgg acgccggcct gggcgtcggc ggagaccgac
     6781 tcgctgctcc acacgcttcc ggccagtcgt agcacggcga gctccgcgtc cgagaactcg
     6841 atgtcggacg gcaggtcgta gtctgcctgc ggaatccggt agcgggcctc ccgcatgtcc
     6901 tgaggatcgg ccggatcgcc gatggtctcc acgggcatcc cgagcgcgcg cagctcgtcc
     6961 ttgtcccgct cgaacatctt ctccagcgaa tcgggcttcg cgcccgcttc gacgcgctgg
     7021 cggtaccccg agacgttgtc gaggatctgc tgcttggtca ggccgatctc cgtggccatc
     7081 agcgccacga cgagattcgt cagccgctcc tccgcgggga tctgcgccgg cgtcatcccc
     7141 ggaccagctc cggcgggacg atgcccagca cgtcgacgac gtaggcgatc gcgtcgtcgc
     7201 cggtcttctc ggggtcgacg acgagaagct ggctgccgac cgtcgccttc gcgacctgcg
     7261 ccatcacctg cggcgggagg gtggaggtgt cgtacgcgac gcccttctcc cacgacgaac
     7321 ctgtcacggc acgggtcgac cacttcacgg cggtgtactg gaacatcgcc agtcccttgc
     7381 cgaccttcgc cccgtcaccc tcgatcaggg tctcggtcac gagcttcttc ggagccgcgc
     7441 cgtcggggat gatgatgccc ggctgcccgc tgggcgcacg caccacggac gggatgccct
     7501 gtgcgtcgtt gaacacgtcc cggccctgcg ccttcggcag cagcacctcc tggatgtcga
     7561 tcgtgccgac gatgctctca tccggaccga gtccgacctg cgcggccatt ccctcgggaa
     7621 ggtccttcgc gggcaccgcg aacgcgaccc gggagccgtt ggtggcgcac tgcagcgccg
     7681 agccgatgcc gccgaactgc tcgctgagcg acttcggcga ccagacgccg gcaccgctgt
     7741 cgatcatctg accggtggcg ccgttgagca gggtgatcga accgacgacg tcctgggtct
     7801 cgtcgcgcac gactgcgccg tcgccgacca cgaggtcgtc gtagaccacc ttcgacgtcg
     7861 gcatcggcga cgtcaggctc accttcgcgg cgccgatgtc accgctcgcc ttgaccgcca
     7921 tcgagagcgc ggcggactcg ctgcgctcgc aaccggcggc gccggatccc ggcgcggtcg
     7981 agcagcccgt caggacgaga gcggacaggg cgagggccga gacggcagcg gtcgttttac
     8041 gcacgcgccc cagtctattc cgaagcgctg tcgccctcgc gcgacgcggc ggcggtgcgc
     8101 gccccgttcg cggccttctg cgcctcgcgc acccgcttgc gcaggttctt gtccgtgatc
     8161 tcgcgatcgc cgaccgcgcc gggagtccac agctccacgt cctcgtcgcc gtagctggac
     8221 ttggaggcgc ggcgcttgac ctcgggagga acggcgcccg gcgccaggcg ccgcgcggtc
     8281 agcaggaaac cggtgtgcgc gaccatcctg tggtcgggtc ggacagccag cccctcgaca
     8341 tgccatccgc ggaccatggt ctcggaggcg tccggatcgg tgaacagccc cgtaccgcgg
     8401 atgtactccg cgacgcggga cagctgagtg gcggtggcga catagcagat caccacgccg
     8461 ccgggcgcga gagcgtcggc gacgacgtcc atgcactccc agggcgcgag catgtcgagg
     8521 acgacgcgat ccaccgtgcc ggcctcgaac tgagcgggca gctcctctgc gaggtcgccc
     8581 acgacgacgt tccaggtgtc gggcgtctcg ccgaagaagg tctcgacgtt cgcgcgcgcc
     8641 acgtccgcga agtcctcgcg ccgctcgaag gacgtcaacc tgccctcgtg accgatcgcc
     8701 cggagcaggg cgagcgagag tgcacccgag ccgacgccgg cctcgacgac gacagcgccg
     8761 gggaagatgt cggcctgcat gacgatctgc gccgagtcct tggggtacac gatcgccgcg
     8821 ccgcgcggca tcgacatcgc gaagtcgcgc agcagcgggc gcagggccag gtagtcgtgt
     8881 ccgccgctgt tggtcacgac cgagccgtcg ggctcgccga tcagctgggt gtgcttgagc
     8941 actccgtggt gggtgtgcag ctccccgtct ccgcgcagcg tgatggtgtg catccgcccc
     9001 ttgggaccgg tcagctgcac gcggtcgccc tctcggaacg gcccgcgggg tcgattggcg
     9061 ctcatgcgtt ctccttggcg tggcggccgg cgaggtcgcg gatgtcggcg gacgtgcgtc
     9121 ctgcgagcgt cggccacagt tcgtgcgcgt ccgagccttc cagggagacg atgtgcggca
     9181 cgcccagggt caccgcaccc gatgcgcgac ccgcggcgac tccggtgccg gaatcctcga
     9241 tcacgacggc atccgcgatg tcgatctcca gcagctcggc ggctcgcagg tacgggtcgg
     9301 ggaagggctt ggggcgcgct acatcatcgc cggcgaccac gaggtcgaag gcgtcgaagt
     9361 cgatcagcga gacgacgtcg agtgccatgc ggcgcagcga catggtcacc agggccgtgg
     9421 ggatgccgtt ggcgcgcagg tctgcgagca gctcgcgagc gccgtgccgg aacgggaccc
     9481 cctcgctgcg gaggcggtgg gcgacggcat ccgtgaggtg ctggacgatg gcgtccgcct
     9541 ccatgtcgac gccgtacttc tgcaggatca gagcgctgtc gtgcagaccg ttgccgacca
     9601 gctggagcgc gtcctcgtgg gtccacgtgc cgccgaacga ctcgaccagg acggtctcgg
     9661 cgtccatcca gtacggctcg gtgtcgacga gggttccgtc catgtcccac aggactgcgc
     9721 tgggcttgtt gctcactgaa cccatgctag ccgggagctg gcccggggcc gatgccgggc
     9781 gactagcctg gagggacact tccggggcgc agaccccgga tgaccggagg gagaacggtg
     9841 gacgctctcg gctcacgcat catcgtcgcc gcgttcgacg gctggaacga cgccggcgag
     9901 gctgccagtg gcgccatcgc cgcactgcgc gaggcgggga gctacgaggc cgtgcactcc
     9961 gtcgatcccg agctgtactt cgactatcag tacacgcggc cctcgtcccg gatggatgcc
    10021 tccggccgcc gtgaactgca ctggccggac gccacgctgt ggcgccccac gagcacgacc
    10081 gagggccctg agctgtggct gctcaccgga tcggagcctg cgcgggcatg gcaggcgttc
    10141 gccacggagt tcatcgacgt ggcactgcgc gacgacgtga ccgggctggt cacgctcggg
    10201 gcgatgctct cggatgtgcc gcacacccgt cccatctcga tcttcgcgtc gagccagaac
    10261 gagcgggtgc gcgctgcgca cggcctggac aagtccacct acgaggggcc cgtgggcatc
    10321 ctgagcgtct tcgagcactt cgccgagcgc gccgagatcc cctccgcgag cctgtgggcc
    10381 agcgtgccgc actacgtcgc gaccgccgca ccctcgccga aggcgacgct ggcgctgctg
    10441 gaccgactcg aggagctcac cggcgtcggc cccgcccgcg agcacc
//
LOCUS       DATMSX010000067         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_10351,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000067 DATMSX010000000
VERSION     DATMSX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10351"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>2558
                     /locus_tag="VN041_02045"
     CDS             13..>2558
                     /locus_tag="VN041_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27861.1"
                     /translation="MLLVAVLRPDDVEVDKLPTLLFVVLRPVDRLEIPLVAVLRPDDV
                     DVDKLLTLLFVVLRPVDRLEIPLVAVLKPDDVEVERLSMLLVAVLNPLDVEVDRLSML
                     LVAVLRPEDVEVERLLTLLFVELRLVDKLEMPLVAVLNPLDVEVDKLSMLLLAVLRPD
                     DVEVDRLLTLLFVVLRLVDRLEIPLVAVLKPDEVDVDRLSMLLVAVLRPDDVEVDKLL
                     TLLFVELRPVDRLVIPLVAVLRPLDVDVERLSMRLLLVLRPDDVEVDRLFTLLFVVLR
                     PLDKLEMPLVAVLRPLDVEVDKLLRLLLVLLRAVDRLEMPLVAVLKPLDVEVDKLLTL
                     LFVVLRPVDRLEIPLVAVLRPDDVDVERLSTRLLTVLSPEDVEVDRLPTLLFVVLKPV
                     DRLEMPLVAVLRPDDVEVDRLLTLLLVVLKPVDKLEIPLVAVLKPDDVDVDKLLTLLF
                     VVLRPVDRLEMPLVAVLKPDDVEVDRLLTLLFVVLKPVDRLEIPLVAVLKPDDVEVDK
                     LLTLLFVVLRPVDRLEMPLVAVLSPDDVDVERLSMRLLLVLRPDDVEVDKLLTLLFVV
                     LRPVDRLEMPLVAVLRPLEVDVDRLSMRLLFVLRPDDVEVDRLPTLLFVVLKPVDRLD
                     IPLVAVLRPDDVDVDRLLTLLLVVLKPVDKLEMPLVAVLKPDDVDVDKLLTLLFVVLR
                     PVDRLEMPLVAVLRPDDVDVDRLLTLLLVVLKPVDKLEIPLVAVLKPDDVDVDKLLTL
                     LFVVLRPVDRLEMPLVAVLRPDDVDVERLVTLLFVVLRPVDRLEMPLVAVLSPLDVDV
                     DRLLTLLFVVLNPVERLEMPLVAVLRPDDVDVERLSMRLLVVLRPDDVEV"
BASE COUNT          437 a          726 c          866 g          529 t
ORIGIN      
        1 gacaagctat cgatgctgct cgtggccgtg ctcagacccg acgacgtcga agtcgacaag
       61 ctaccgacgt tgctgttcgt ggtgctcagg cccgtggaca ggctcgagat cccgctcgtc
      121 gccgtgctga gacccgacga cgtggacgtc gacaagctgc tgacattgct gttcgtggtg
      181 ctcagacccg tcgacaggct ggagataccg ctggtggccg tgctcaaacc agacgacgtc
      241 gaagtcgaaa ggctatcgat gctgctcgtg gccgtgctca acccgctcga cgtcgaagtc
      301 gacaggctgt cgatgctgct ggtagcggtg ctcagacccg aggacgtcga ggtcgagagg
      361 ctgctcacgc tgctgttcgt ggagctgagg ctcgtggaca agctggagat gccactggtt
      421 gcagtgctca atccgctcga cgtcgaggtc gacaagctat cgatgctgct cctagctgtg
      481 ctcagaccgg acgacgtcga agtcgacagg ctgctcacat tgctgttcgt ggtactcagg
      541 ctcgtggaca ggctggagat accactggtg gccgtgctca aaccagacga ggtcgacgtc
      601 gacaggctgt cgatgctgct cgtggccgtg ctcagacccg acgacgtgga ggtcgacaag
      661 ctgctgacgt tgctgttcgt ggagctcagg cccgtcgaca ggctcgtgat accgctggtg
      721 gcggtgctca gaccgctcga tgtcgatgtc gaaaggctgt cgatgcggct gctgctggtg
      781 ctcagacccg acgacgtcga ggtcgacagg ctgttcacgt tgctgttcgt cgtgctcagg
      841 ccgctggaca agctcgagat gccgctggtc gccgtgctca ggccgctcga cgtcgaagtc
      901 gacaagctgc tcaggttgct gttggtgctg ctcagagccg tagacaggct cgagatgccg
      961 ctggttgccg tgctcaagcc gctcgacgtc gaggtcgaca agctgctgac attgctgttc
     1021 gtggtgctca gacctgtgga caggctggag ataccactgg tcgccgtgct caggcccgac
     1081 gacgtagatg tcgagaggct gtcgacgcgg ctattgacgg tgctcagccc cgaggacgtc
     1141 gaggtcgaca ggctgcccac cttgctgttc gtggtgctca agcccgtgga caggctggag
     1201 atgccgctcg tggccgtgct caggccggac gacgtcgaag tcgacagact gctgacattg
     1261 ctgttggtcg tgctcaagcc ggtggacaag ctggagatac cactcgtggc cgtgctcaag
     1321 ccagacgacg tcgatgtcga caagctactg acgttgctgt tcgtggtgct caggccagtc
     1381 gacaggctgg agatgccgct ggtggccgtg ctcaaaccgg acgacgtcga agtcgacagg
     1441 ctgctgacat tgctgttcgt cgtgctcaag ccggtggaca ggctggagat accactcgtg
     1501 gccgtgctca agccggacga cgtcgaagtc gacaagctac tgacgttgct gttcgtggtg
     1561 ctcaggcccg tcgacaggct ggagatgcca ttggtcgccg tgctcagccc ggacgacgtc
     1621 gatgtcgaaa ggctgtcgat gcggctgctg ctggtgctca ggccggacga cgttgaggtc
     1681 gacaagctgc tgacgttgct gttcgtcgtg ctcagacctg tggacaggct cgagatgccg
     1741 ctcgtggcgg tgctcagacc gcttgaagtc gacgtcgaca ggctgtcgat gcggctgttg
     1801 ttcgtgctca ggccggacga cgtggaggtc gacaggctgc ccacattgct gttcgtcgta
     1861 ctcaagcccg tggacaggct cgatattccg ctggtcgccg tgctcagacc cgacgacgtg
     1921 gacgtcgaca ggctgctgac attgctgttg gtcgtgctca agccggtgga caagctggag
     1981 atgccgctcg tggccgtgct caagccagac gacgtcgatg tcgacaagct actgacgttg
     2041 ctgttcgtgg tgctcaggcc cgtcgacagg ctggagatgc cactggtcgc cgtgctcagg
     2101 ccggacgacg tcgatgtcga caggctgctg acattgctgt tggtcgtgct caagccggtg
     2161 gacaagctgg agataccact cgtggccgtg ctcaagccag acgacgtcga tgtcgacaag
     2221 ctactgacat tgctgttcgt ggtgctcagg cccgtcgaca ggctggagat gccgctggtc
     2281 gccgtgctca gaccggacga cgtcgatgtc gaaaggctgg tgacattgct gttcgtcgtg
     2341 ctcagacctg tggacaggct cgagatgcca ctggtcgccg tgctcagccc gctcgacgtc
     2401 gatgtcgaca ggctactgac gttgctgttc gttgtgctca accccgtgga gaggctggag
     2461 atgccactgg tagcggtgct caggccagac gacgtggacg tcgagaggct gtcgatgcgg
     2521 ctgttggtcg tgctcagacc cgacgacgtc gaggtcga
//
LOCUS       DATMSX010000068        11273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_142893,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000068 DATMSX010000000
VERSION     DATMSX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142893"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VN041_02050"
     CDS             complement(<1..1113)
                     /locus_tag="VN041_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWU27862.1"
                     /translation="MEIAGIIGILVIVGVAVVAVIVIGLIMLLFARSWIKVARADEAL
                     VISGRKQKVQRAVLNADGSTSSELAESPVTVIVNGKSLVNPITQRHEIISLRSRQVLL
                     NAEAQSLDSVTLNVDGVAIVKIGSDPLYVRRAAERFASQDKAIEQFTTEQLEGALRGI
                     VATLSVVELMRERKKFSDQIAADVSSELADQGLILDSFQIKGITDKVGYIQSLGAPEI
                     QAKRQAAEISQTNADRAINQKNIANQEANLIEQTALDTNTANANAGIGRARAEAEQAE
                     QLARAQAEQAVLQQQAENKQAQLDADVKRVADAQRYEAETRAQAELFTREKSAEAAAI
                     EQVKQAEARTRIAEQQAQADKARAAGEAAAAEAKATG"
     gene            complement(1192..1620)
                     /locus_tag="VN041_02055"
     CDS             complement(1192..1620)
                     /locus_tag="VN041_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU27863.1"
                     /translation="MTDRQLALQAWESLFRAQHELFGEMTEDFDGAPITQAEYDVLLT
                     VVRSCEMTARLRDITANSLISQPSVSRLIDRMVSRELVTKASDPEDGRGAVVTATDAG
                     ARAFRTLAASHGRSIAERMAKLDDDELRTLLALTEKLRGR"
     gene            complement(1833..2717)
                     /locus_tag="VN041_02060"
     CDS             complement(1833..2717)
                     /locus_tag="VN041_02060"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWU27864.1"
                     /translation="MTAAPEEITSSLVRSLVSEQFPQWSELPIRPVEVQGWDNRTFQL
                     GDRMSVRLPSTDGYVAGLVREEQTLAALGSRLRVAIPRVVATGSPSAAFNRPWSVRAW
                     IDGQTLTTVGARARDRAISGVGDALRELQACDTAGGPWAGSASAYRGCHVSAVGEQVQ
                     DQLPLLSGRQADGCRAVWDAAVTTVWADPPVWVHGDVAPGNMLFDTDGRLTALIDFGQ
                     TCVGDPACDLAFAWLCCSSRERDLLRERLGLPEDAWLRGAAWALWKALISSPEEMLAK
                     YGRSRDAVLTDVADLPAA"
     gene            2780..3298
                     /locus_tag="VN041_02065"
     CDS             2780..3298
                     /locus_tag="VN041_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU27865.1"
                     /translation="MTPTDGTKRLRRSARAILLDEDDAVLLCRFGFPHPAVPTDAMVV
                     WAAPGGGLEPGEGPLQALRRELLEETGFTLDVDATHVWRQEVIAPGIGDGMDGVRNDY
                     FLVRSPRFQPRGLLSDEAIAAEGITEMRWWTLPEIVAYRGSDLFSPRDLATPLARLIA
                     GDIPHSPVQLGL"
     gene            complement(3321..4103)
                     /locus_tag="VN041_02070"
     CDS             complement(3321..4103)
                     /locus_tag="VN041_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU27866.1"
                     /translation="MDVQQTLAAIGEPTRFRIVNMLAEGPRTVGEIATALGALQPQTT
                     KHIQALETAGVVKVTRLGRRRLASLDRATFREIAEWADGASVESEDDRALGAYVQKVA
                     EVTAHAGNDAALTTVELTRRLRASRSTVWRAWTDPELAARWWAPRHFEAVRCEIRPEV
                     GARVELVLREADGTIYTSSGTVREVQPERRLTFDLSPMDAQGDTLLTVDVEVRLEGED
                     GTSLHLTIRAKDVTAATAPMAAGLEPGWSQQLDRLEALLAPR"
     gene            4220..4678
                     /locus_tag="VN041_02075"
     CDS             4220..4678
                     /locus_tag="VN041_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWU27867.1"
                     /translation="MHTIERTVSASPETVWRLWTTADGISSWWAPDGFRTAVDTLDLR
                     PGGELVYTMTAEAPEQVAFLEQNGLPLSTQSRKVFTEIVEPNRISYRSVIDFVPDHEP
                     YEQLTEVDLQPSGEGTLVVMRVEDMHDQVWTERLLAGRANELDNLARLVG"
     gene            4772..5806
                     /locus_tag="VN041_02080"
     CDS             4772..5806
                     /locus_tag="VN041_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HWU27868.1"
                     /translation="MGLLAWYRSTGADLPFQSPLPAHSGVAMEGYFWRITAPEAGRVL
                     IALNGVNRGPHGPWATLGLAAHPGGSLRVDAHPTASADLARIGAHAGDAFHGDDRRLR
                     VDLGSDAQLDVTIDDPLPWPHRAFGGSSVFQTVPALNQYWHPWLLGGRVNGIATIGDE
                     TWELHDAQVYAEKNWGREGFPDAWWWGQAQGFDERDACVAFAGGQVRSGPIRTEVTGL
                     VVRLPGGRVIRLGNPGISPVRAVVDDERWMLHGRGFGWTIDVAGTAPRAASHVLPVPL
                     PSQERNTAGALEHLAGALEVTVRRRGRVVWAGRSVLAGLEHGGLDRAKAELVRRGLDA
                     SATHAAPIEE"
     gene            complement(5922..7793)
                     /locus_tag="VN041_02085"
     CDS             complement(5922..7793)
                     /locus_tag="VN041_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWU27869.1"
                     /translation="MHRHPHSGSPEPRWCSCAVPSDVEEQRRSGLSRRGFLTGVTAAS
                     ALAAAGWVARPEQASAAQGGVGKSLTITIMGTSDLHSHAVNWDYYKDAAYADSKGNAV
                     GLARVSSVVKQIRADRGSDRTLLFDAGDTIQGTPLGFYYATVEPITKTGAVHPMAAQM
                     NALGYDAVALGNHEFNYGLEFLDTWIKQMDAPVLGANAVRAGTNIPAFTPYTIKTLHP
                     KGAKPVRVGVLGLTNPGIVIWDKANVSGKVEVLDIVATAARWVPIIRAQGVDVVVVSV
                     HSGDSGTSSYGDALPNENAAALLAAQVPGIDAILFGHAHKDIPQRFVTGPDGRQVVLS
                     EPMCWGQRLSVFDLELQQVKGQWQVVSRKATNLNTNTVEDDPAMVALIKAQHDAVVAY
                     VNKPVATSTEELSAAESCWKDTAILDYVNKVQVDTVTAAIQGTPEAALPVISIAAPFN
                     REATFPAGEVTIRDVAGLYIYDNTLMASVLTGAQIKDYLEYSAKYFQQVAPDAPVEPA
                     SWTNALNTPDYNYDQFSGVTYDVDISQPQGSRIVNLSWDGAPIAADAKFVVAINNYRQ
                     SGGGGFPHVTTAPVVYNAQVAIREAIVQYASDAGVIDPKDFHVVNWKLVRGGAPVFS"
     gene            7890..8255
                     /locus_tag="VN041_02090"
     CDS             7890..8255
                     /locus_tag="VN041_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU27870.1"
                     /translation="MSADKRKCGYTPDSSFVELAVEVFGMLADATRVRIILALQDAEE
                     LSVNHLADIVDKSPAAVSQHLAKLRLARIVATRQDGQRVFYRLENEHASELVASAIFQ
                     AEHTLGGTPRHHHQDRAGE"
     gene            8252..9289
                     /locus_tag="VN041_02095"
     CDS             8252..9289
                     /locus_tag="VN041_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWU27871.1"
                     /translation="MTAQHVHHEHDHAHESHRHDHGHDHAHGHAHGDHHHEHPTGFKG
                     FLYGLFVPHTHDAADSIDDAMEANAAGIRALKVSLILLLITTVLQGAVVVFSGSVALL
                     ADTIHNFSDALTAVPLWIAFVLGRRAASRRYTFGFGRAEDLAGLFIVFVVALSAIVAA
                     WQSIERIIHPQSLTNLGWVIAAGVIGFLGNETVAIYRIRVGRRIGSAALVADGVHART
                     DGFTSLAVVIGGIGVLLGFPLADPIIGLIISAAIFVLLIGTVRSVGRRLLDGVEPELV
                     DRAEHALEHVDGLVGIERLRLRWVGHRLQGDAVVRVESGHPDAADAAERSMREHLPNV
                     DDFLVRVRVAS"
     gene            9317..9622
                     /locus_tag="VN041_02100"
     CDS             9317..9622
                     /locus_tag="VN041_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWU27872.1"
                     /translation="MSEAVEYLITALDGTDAAAPERRLAVRDEHLAGAQKLAERGELL
                     SGGAILDDDGRMIGSSMHVAFADEAAFRAWYDAEPYILGGVWQDISIRPMKIFRPVL"
     gene            complement(9632..10282)
                     /locus_tag="VN041_02105"
     CDS             complement(9632..10282)
                     /locus_tag="VN041_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27873.1"
                     /translation="MNAEVSPRVEATRRAGRARVITLARIKLPTWNGALSALTSELRE
                     DAGLSEGAFRTLFPTDRDLLRAVDDELLEEIAQRVRTAAESFDPARYTEEELPLALSV
                     ALADARPLDWSSLNIRLRVRLLAASTGAHSEEVLASERAFLPTMLEAFELLLSRIHHR
                     FTWSPALGVRVVILTYERSFESWIMSGRNEQSFAESSYIKHTLPELLLGMSAPSQT"
     gene            complement(10296..10421)
                     /locus_tag="VN041_02110"
     CDS             complement(10296..10421)
                     /locus_tag="VN041_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27874.1"
                     /translation="MTPTLAGEARGIQGSQRGGTLTVAAVRLHRGSPERMPIPSA"
     gene            10420..10698
                     /locus_tag="VN041_02115"
     CDS             10420..10698
                     /locus_tag="VN041_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27875.1"
                     /translation="MTEKQNLQGRRWLLGITLMAAEQASRVLEVCASVEGDDAALNRA
                     LCEEFDINPIDAEVVLSLQVRRFTPASIARMRSELAEMERRIELVGEH"
     gene            10784..>11273
                     /locus_tag="VN041_02120"
     CDS             10784..>11273
                     /locus_tag="VN041_02120"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWU27876.1"
                     /translation="MTMTAMQDDDALEPQSSAPQRARIEAAPRPVDLDAAEEAGRLFL
                     KALGIDPDAADALDLARTPRRFAEAYAELLQPEPFDFTTFANSEGYDELVLIRDIPVR
                     SICEHHLLPFTGVAHVAYLPADRVVGLSKIPRMVDHYARRPQTQERLTVQIADALAAR
                     LQP"
BASE COUNT         1703 a         3836 c         3867 g         1867 t
ORIGIN      
        1 accggtcgcc ttcgcctcgg cggcggcggc ttcaccggcg gcgcgtgcct tgtcggcctg
       61 cgcctgctgc tcggcgatgc gggtgcgtgc ctcggcctgc ttgacctgct cgatcgcggc
      121 ggcctcggcc gacttctcac gggtgaacag ttcggcctgc gcgcgggtct cggcctcata
      181 ccgctgcgcg tcggcgacac gcttgacgtc ggcatccagc tgggcctgct tgttctcggc
      241 ctgctgctgg aggacggcct gctcggcctg ggcgcgggcg agctgctcgg cctgctcggc
      301 ctcggcacgg gcgcggccga tcccggcgtt ggcgttggcg gtgttggtgt cgagcgcggt
      361 ctgctcgatc aggttcgctt cctggttggc gatgttcttc tggttgatcg cacggtcggc
      421 gttggtctgc gagatctccg cggcctggcg cttcgcctgg atctcgggag cacccagcga
      481 ctggatgtag ccgaccttgt cggtgatgcc cttgatctgg aaggagtcga ggatcagacc
      541 ctggtcggcc agctcgctgg acacgtcggc ggcgatctgg tcggagaact tcttgcgctc
      601 gcgcatcagc tccacgacgg acagggtggc gacgatgcca cgcagggcgc cttcgagctg
      661 ctcggtggtg aactgctcga tggccttgtc ctgcgaggcg aagcgctcag cggcgcggcg
      721 cacgtagagg gggtcggagc cgatcttcac gatcgcgacg ccgtcgacgt tgagggtcac
      781 gctgtccagc gactgggcct cggcgttcag cagcacctga cgggaacgca gcgagatgat
      841 ctcgtggcgc tgcgtgatcg ggttgaccag ggacttgccg ttgacgatga ccgtgacggg
      901 agactccgcc agctccgagc tggtgctgcc gtcggcgttg aggacggcgc gctgcacctt
      961 ctgcttgcga cccgagatca ccagcgcctc gtccgcgcgt gcgaccttga tccagctgcg
     1021 tgcgaacagc agcatgatga gtccgatgac gatcacggcc acgacggcta cgccgacgat
     1081 gaccaggatg ccgatgattc cggcgatctc catagaagtg ccctttcccc tcagaaagcg
     1141 tgtttcagct ccaccctgcc agaagtcggg gtgcggtgcc gatggggaga actaccggcc
     1201 gcgcagcttc tcggtcagag cgagcagcgt gcggagctcg tcgtcgtcga gcttcgccat
     1261 ccgctctgcg atcgaccgcc cgtgcgaggc ggcgagggtg cggaaggcgc gcgcgccggc
     1321 atccgtcgcc gtcacgaccg cgccgcggcc gtcttccgga tcggatgcct tggtgaccag
     1381 ctcgcgtgag accatccggt cgatcagccg cgagacgctg ggctggctga tcagcgagtt
     1441 cgcggtgatg tcgcgcagtc gcgcggtcat ctcgcaggag cgcaccacgg tgagcaggac
     1501 gtcgtattcg gcctgggtga tcggtgcgcc gtcgaagtcc tcggtcatct cgccgaagag
     1561 ctcgtgctgt gcccggaaca ggctctccca ggcctgcagg gcgagctggc ggtcagtcat
     1621 gtcatcagcg tagtgtggcg cgccgacgtc gtgctcgcgg cacaagtaag ggccggtcgg
     1681 agaggcttcc ggccggccct tgtcccttgc accaagagtg tcctgcaatc acatgcggtt
     1741 catgccacag caaacattca ccgtgcgatg agtgtataac ggcgaggtaa cgaatgcaac
     1801 ggtttggtca cggagatcgg agatcttcgg tctcatgccg ccggcagatc cgccacgtcg
     1861 gtcagcacgg catcacgtga gcgcccgtat ttggcgagca tctcttcggg ggagctgatc
     1921 agggccttcc acagagccca ggccgctccc ctcaaccacg cgtcctcggg caggcccagc
     1981 cgctcgcgca acagatcacg ctcgcgcgaa ctgcagcaca accacgcgaa ggccaggtcg
     2041 caggcgggat cgccgacgca ggtctgtccg aagtcgatga gtgccgtcag tcgaccatcg
     2101 gtgtcgaaca acatgttgcc tggcgcgacg tccccatgaa cccacaccgg cgggtcggcc
     2161 cacacggtgg tcacggcagc atcccacact gcccgacatc cgtcagcctg gcggccggac
     2221 aggagcggga gctgatcctg tacctgctcg ccgaccgcgc tgacgtgaca gccgcgatag
     2281 gccgatgcgc ttcccgccca ggggccgccc gccgtgtcgc aggcctgcaa ctccctcagg
     2341 gcgtcgccca cgccgctgat ggcgcggtct cgcgctctcg ctccgactgt ggtcaaggtc
     2401 tgtccgtcga tccacgcgcg aacggaccaa ggccggttga atgccgccga gggagacccg
     2461 gtcgccacca cgcgggggat cgcgacgcga aggcgggagc cgagggctgc gagggtctgc
     2521 tcctcccgca ccaggccggc gacgtagccg tccgtgctcg ggaggcggac agacatacgg
     2581 tcaccgagct ggaaggtgcg gttgtcccac ccctggactt ccaccggcct gatcggcagc
     2641 tccgaccact ggggaaactg ctccgagacc agggagcgaa ccagcgaaga agtgatctcc
     2701 tcaggagcag cggtcatcgc gccagcgtag tggacgagcc ccgcgagcag cgtcgccggg
     2761 gtgcgagatg atcgacgtca tgactccgac ggacgggacg aagcgcctcc ggcgctcggc
     2821 gagggcgatc ctcctcgatg aggatgacgc ggtgctgctg tgccgattcg gattcccgca
     2881 tccggcggtc ccgaccgacg ccatggtcgt gtgggcggct ccagggggag ggctcgaacc
     2941 gggagagggg ccgctccagg ccctgcgccg agagcttctc gaagagaccg gtttcacgct
     3001 cgacgtcgac gcgacccatg tctggcggca ggaggtcatc gcgcccggga tcggtgacgg
     3061 catggacggg gtgcgcaacg actacttcct ggtccgatca ccgcgcttcc aaccccgcgg
     3121 actcctgtcc gacgaagcca tcgccgccga agggatcacc gagatgcgct ggtggacact
     3181 gccggagatc gtcgcgtacc gcggatcgga cctgttctcg ccccgggacc tcgcgacccc
     3241 gctggcgcgc ctcatcgcag gcgacatccc tcattccccg gtccagctcg ggctgtgagc
     3301 acgatcagtt ctctgcggga tcagcgggga gcgaggagcg cttcgagacg gtcgagctgc
     3361 tgagaccatc cgggttccag gccggcggcc atcggtgcgg tcgccgcggt cacgtctttc
     3421 gccctgatgg tcagatgcag tgaggtccca tcctcgccct ccagccggac ctcgacgtcg
     3481 acggtgagaa gcgtgtcgcc ctgcgcatcc atgggtgaca ggtcgaacgt cagccgtctc
     3541 tcgggctgca cttcacgcac ggttcccgag gacgtgtaga tcgtgccgtc cgcctctcgc
     3601 aggacgagtt ccacgcgagc cccgacctcg ggacggatct cgcacctcac cgcctcgaaa
     3661 tgacgcggcg cccaccatcg cgcagcgagt tccgggtccg tccacgctcg ccagacggtg
     3721 ctgcgcgagg cgcgcagtcg ccgggtgagt tcgacggtcg tgagcgccgc gtcgttgccg
     3781 gcgtgggcgg tgacctccgc caccttctgc acgtacgcgc cgagcgcccg atcgtcctcc
     3841 gactcgaccg acgccccgtc ggcccactcg gcgatctcgc ggaacgtcgc ccggtccagg
     3901 ctggccagac gccgccgccc gagtcgggtc accttcacga cgccggcggt ctccagtgcc
     3961 tggatgtgct tcgtcgtctg cggctgcagc gcacccaggg cagtcgcgat ctcgccgacc
     4021 gtgcggggcc cctcggcgag catgttcacg attcggaacc gcgtcggttc gccgatggcc
     4081 gcgagcgtct gctgtacgtc cacatcgcct cctcactgag atcatacttg acatattcgt
     4141 tgagacgaat acatagtgga tcgcatcgaa gttcagaacc ggatgcccag cgtccacgac
     4201 ccgtcaggag aatcccgaca tgcacaccat cgagcgcacc gtgtccgcat cacccgagac
     4261 cgtctggcgg ctgtggacca cagccgatgg catctccagc tggtgggcac ccgacggctt
     4321 ccgcaccgcc gtcgacaccc tcgacctgcg tcccggcggg gaactggtct acaccatgac
     4381 agccgaggcg ccggaacagg tcgccttcct cgagcagaac ggcctgccgc tgagcacgca
     4441 gtcccgcaag gtcttcaccg agatcgtcga gccgaatcgg atctcctatc gctctgtcat
     4501 cgacttcgtc ccggatcacg agccgtatga gcagctgacc gaggtggacc ttcagccgtc
     4561 aggagagggc acgctcgtgg tcatgcgcgt cgaggacatg cacgatcagg tgtggaccga
     4621 gcgtctgctc gccgggcggg cgaatgaact cgacaacctc gcccggctcg tcggctgatc
     4681 cggcggacgg cggcgtcgct cgcgatgcgg agtcgcgcgg tgcgcgcgca tcccggttcc
     4741 cctccgcccc gtgccgtgct agacaacgct catgggcctg ctcgcgtggt accggtcgac
     4801 cggggcggat ctgccgttcc agagtccgct gcccgcgcat tccggcgtcg cgatggaggg
     4861 ctacttctgg cgcatcaccg cgccagaagc cggccgagtg ctcatcgccc tgaacggcgt
     4921 gaaccgcggt ccgcacgggc cctgggcgac gctcggcctc gcagcgcacc cgggcggatc
     4981 cctgcgcgtg gatgcgcatc ccacggcatc cgctgacctc gcccgcatcg gagcgcatgc
     5041 cggtgacgcc ttccacggcg acgatcggcg gctgcgcgtg gatctcggct cggatgcgca
     5101 gctggacgtg accatcgatg acccgctgcc ctggccgcat cgcgcgttcg gcggctcgag
     5161 cgtgttccag accgtgcccg cgctgaacca gtactggcat ccgtggctgc tcggcgggcg
     5221 cgtgaacggc attgcgacca tcggagatga gacgtgggag ctgcacgacg cccaggtcta
     5281 cgccgagaag aactgggggc gggagggatt ccccgatgcc tggtggtggg gtcaggccca
     5341 ggggttcgac gagcgcgacg cgtgcgttgc cttcgcgggc ggtcaggtgc gctcggggcc
     5401 gatccgcacg gaggtgaccg gtctggtcgt acgcctgccg ggagggcgcg tcatccgcct
     5461 cggcaacccg ggcatcagcc cggtgcgtgc ggtcgtcgac gacgaacgat ggatgctgca
     5521 cggacggggc ttcggatgga cgatcgacgt cgccggcacg gcgccgcgcg cggcatccca
     5581 cgttctgccg gtgccgctgc cctcgcagga gcgcaacacg gcaggcgcgc tcgaacatct
     5641 cgccggcgcg ctcgaggtga ccgtgcgccg caggggacgt gtcgtctggg cgggacgctc
     5701 tgtgctggcg ggtctcgagc acggtggcct cgaccgcgcg aaggcggagc tggttcgccg
     5761 agggctggat gcctcggcca cccacgccgc accgatcgaa gagtgacgcg ttcggtctcc
     5821 accgttccga gcaactccgg agaaacgccc tcgccgaacc cgttcagggc tctgcgaggg
     5881 cgtttcgccc ccggatctcc ggagttgtgg cggccgaccg atcagctgaa gaccggcgcc
     5941 ccgcctcgca cgagcttcca gttcacgacg tggaagtcct tcgggtcgat gacgccggca
     6001 tccgaggcgt actgcacgat ggcctcgcgg atcgcgacct gcgcgttgta gaccaccggc
     6061 gcggtggtga cgtgcgggaa gccgccgccg ccggactgac ggtagttgtt gatcgcgacg
     6121 acgaacttcg catccgccgc gatcggggcg ccgtcccagg acaggttcac gatccgcgac
     6181 ccctgcggct gggagatgtc gacgtcgtag gtcacgccgg agaactggtc gtagttgtag
     6241 tccggcgtgt tcagcgcgtt cgtccagctc gccggctcga cgggggcatc cggcgcgacc
     6301 tgctggaagt acttcgcgga gtactccaga tagtccttga tctgcgcgcc ggtcagcacc
     6361 gaggccatca gggtgttgtc gtagatgtac agaccggcga catcccggat cgtcacctcg
     6421 cccgcgggga aggtcgcctc ccggttgaac ggcgccgcga tcgagatgac gggaagtgcc
     6481 gcctcggggg tgccctggat cgccgcggtg acagtgtcga cctgcacctt gttgacgtag
     6541 tcgaggatcg cggtgtcctt ccagcacgac tccgcggccg acagctcctc ggtcgacgtg
     6601 gcgacgggct tgttgacgta ggcgaccacg gcgtcgtgct gcgccttgat cagggcgacc
     6661 atcgccgggt cgtcctcgac ggtgttcgtg ttcaggttgg tcgccttccg cgagaccacc
     6721 tgccactggc ccttcacctg ctgcagctcc agatcgaaca cgctcaggcg ctggccccag
     6781 cacatcggct cgctgagcac gacctgcctt ccgtccggtc cggtcacgaa ccgctgcggg
     6841 atgtccttgt gcgcgtgccc gaacaggatc gcgtcgatcc ccggcacctg cgcggccagg
     6901 agggcggcgg cgttctcgtt gggcagcgcg tccccgtacg acgaggtgcc gctgtcaccg
     6961 gagtgcacgc tgacgacgac gacgtccaca ccctgtgcgc ggatgatcgg cacccagcgg
     7021 gctgcggtcg ccacgatgtc gagcacctcg accttgccgc tgacgttcgc cttgtcccag
     7081 atcacgatgc cgggattggt gagcccgagc acgccgacgc gcacgggctt cgcgcccttc
     7141 gggtgcagag tcttgatcgt gtacggcgtg aaggcgggga tgttcgtgcc ggcgcgcacc
     7201 gcgttggcgc cgagcacagg ggcgtccatc tgcttgatcc acgtgtccag gaactcgaga
     7261 ccgtagttga actcgtggtt gccgagggcg acggcgtcgt agccgagcgc gttcatctgc
     7321 gctgccatcg gatgcacggc accggtcttg gtgatgggct cgaccgtcgc atagtagaag
     7381 cccagcgggg tgccctggat ggtgtcgccg gcgtcgaaca gcagcgtgcg gtcgctaccg
     7441 cggtcggcgc ggatctgctt caccacgctc gagacacggg cgagtccgac cgcgttcccc
     7501 ttgctgtccg cgtacgcggc gtccttgtag tagtcccagt tgaccgcgtg cgagtggagg
     7561 tcggaggtgc ccatgatcgt gatcgtgagg gacttgccga caccgccctg cgcagcggat
     7621 gcctgttccg gacgtgccac ccatccggcc gccgcgagcg cgctggccgc ggtgacgccg
     7681 gtgaggaatc cacgacggct cagcccggag cggcgctgct cctcgacgtc cgaggggacg
     7741 gcgcaggagc accagcgggg ttcgggggag ccggagtggg ggtggcgatg catttcatct
     7801 gtcatgaacc tcgatgcaac caccgcccgc ggtcgaggac aagagacttc tggtgaacga
     7861 acgcagacga attgccttag tatctgcgta tgagtgcaga taagcggaaa tgcggataca
     7921 cgccggacag ctccttcgtc gagctcgccg tcgaggtgtt cgggatgctg gccgacgcca
     7981 cgcgcgtgcg gatcatcctc gccctgcagg atgccgagga gctctccgtc aatcacctcg
     8041 ccgacatcgt cgacaagtcg ccggcggccg tgtcgcagca cctcgccaag ctgcgcctgg
     8101 cgcgcatcgt cgccacccgg caggacggac agcgggtgtt ctaccggctc gagaacgagc
     8161 acgcgtccga gctcgtggca tccgcgatct tccaggccga gcacacgctc ggcggcaccc
     8221 cgcgtcacca ccatcaggat cgagcgggcg aatgaccgcg cagcacgtgc accacgagca
     8281 cgaccacgcc cacgaatctc accgtcacga tcacggtcac gatcacgccc acggtcatgc
     8341 gcacggcgac caccaccacg agcatccgac cggcttcaag ggcttcctct acggcctgtt
     8401 cgtgccgcac acccacgacg cggccgactc gatcgacgat gccatggagg cgaacgccgc
     8461 gggcatccgc gcgctgaagg tcagcctgat cctgctgctg atcaccacgg tgctgcaggg
     8521 cgccgtcgtc gtgttcagcg gctcggtcgc gctgctcgcc gacacgatcc acaacttctc
     8581 cgatgcgctc acggcggttc cgctctggat cgccttcgtg ctgggccgtc gcgcggcgtc
     8641 ccgccggtac accttcgggt tcggtcgtgc cgaggacctc gccggcctgt tcatcgtgtt
     8701 cgtggtggcg ctgtctgcga tcgtcgcggc ctggcagtcg atcgagcgca tcatccaccc
     8761 gcagtcgctg acgaacctgg gctgggtgat cgcggccggg gtcatcggat tcctcggcaa
     8821 cgaaaccgtc gcgatctacc gcatccgcgt cggccgtcgc atcggatcgg cggcgctcgt
     8881 cgccgacggc gtgcacgccc gcaccgacgg cttcacctcg ctggccgtcg tgatcggcgg
     8941 catcggcgtg ctgctcggct ttcccctggc cgacccgatc atcggcctga tcatctcggc
     9001 ggcgatcttc gtgctgttga tcggcaccgt ccgcagtgtc ggccgacgcc tgctggacgg
     9061 cgtcgagccg gagctcgtcg accgggcgga gcacgctctg gagcatgtcg acggtctggt
     9121 cgggatcgag cgcctccgtc tgcgctgggt cggccaccga ctgcagggcg atgcggtggt
     9181 ccgggtcgag tccgggcatc cggatgccgc ggatgctgcg gagcgcagca tgcgcgagca
     9241 cctgcccaac gtcgacgact tcctggtgcg cgtgcgcgtc gcatcctgac cgttgccgcg
     9301 gccctacgct gatcccatga gtgaagcagt cgaatacctc atcaccgcgc tggacggcac
     9361 cgacgcagcc gctcccgagc gtcgtctcgc cgtgcgtgac gagcacctcg ccggggcgca
     9421 gaaactcgcc gagcgcggtg agctgctcag cggcggcgcc atcctcgacg acgacggcag
     9481 gatgatcggc tcgtccatgc acgtggcctt cgccgacgag gccgcattcc gcgcctggta
     9541 cgacgccgag ccgtacatcc tcggcggggt gtggcaggac atcagcatcc ggccgatgaa
     9601 gatcttccgt ccggtgctct gagcggaacg ctcaggtctg ggagggcgcg ctcatgccca
     9661 ggagcagctc gggcagcgtg tgcttgatgt acgacgactc cgcgaacgac tgctcgttgc
     9721 ggcccgacat gatccatgac tcgaacgagc gctcataggt gaggatcacg acgcgcacgc
     9781 cgagcgccgg tgaccacgtg aaccggtggt ggatgcgcga gagcagcagc tcgaacgcct
     9841 cgagcatggt cgggaggaac gcgcgctccg atgcgagcac ctcctcgctg tgcgcgccgg
     9901 tcgatgcggc gagcagccgc acccgcagcc ggatgttcag cgagctccag tccagcggcc
     9961 gggcgtcggc gagtgcgacc gagagggcga gcggcagctc ctcctccgtg taccgggcgg
    10021 gatcgaagct ctccgccgca gtgcgcaccc gctgcgcgat ctcctcgagc agttcgtcgt
    10081 ccaccgcccg cagcaggtcg cggtcggtgg ggaacagcgt gcggaacgcg ccttcgctga
    10141 gaccggcgtc ctcgcgcagc tcggaggtga gcgcggagag ggctccgttc cacgtcggca
    10201 gcttgatgcg ggcgagcgtg atcacgcgcg cgcggcctgc ccgtcgggtc gcctcgactc
    10261 gggggctgac ttccgcgttc atcctggcat ccgttctacg cgctcggtat cggcatccgc
    10321 tcgggggagc cgcggtgcag gcgcacggcg gcgactgtca gcgtaccgcc gcgctgcgac
    10381 ccctggatgc cgcgcgcctc tccggctagg gtcggcgtca tgacggagaa gcagaacctg
    10441 cagggccgcc ggtggctgct cggcatcaca ctgatggccg ccgagcaggc gtccagggtc
    10501 ctcgaggtgt gcgcgtcggt cgagggcgat gacgccgcgc tcaaccgtgc gctgtgcgag
    10561 gaattcgaca tcaacccgat cgacgccgag gtggtgctct cgcttcaggt gcgacggttc
    10621 accccggcct cgatcgcgcg gatgcgctcg gagctcgccg agatggagag gcggatcgag
    10681 ctggtcgggg agcactgacc ctcccacttc taaaaaagat aaggcttgca tttagaagtc
    10741 gatcgggtgt ctgatggagg aggagaggca caggaggtcg gccatgacga tgacggcgat
    10801 gcaggacgat gatgcgctgg agccgcagag cagcgcaccg caacgcgctc ggatcgaggc
    10861 cgcgccgcgg ccggttgacc tggatgcggc agaggaggcc ggcaggctgt tcctgaaagc
    10921 gctcggcatc gatccggatg ccgcggatgc cctggatctg gcgcgcacgc cgcgccgctt
    10981 cgccgaggcc tatgccgaac tgctgcagcc ggagcccttc gacttcacca ccttcgcgaa
    11041 ctccgagggg tacgacgaac tcgtgctgat ccgcgacatc ccggtgcgct cgatctgcga
    11101 gcaccacctg ctgccgttca ccggcgtcgc acacgtggcc tacctgcccg ccgatcgcgt
    11161 cgtcgggctc tcgaagatcc cgcggatggt cgatcattac gcgcgtcgcc cgcagacgca
    11221 ggagcggctg accgtgcaga tcgccgacgc cctggccgcg cggctccagc cgc
//
LOCUS       DATMSX010000069        13204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_67272,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000069 DATMSX010000000
VERSION     DATMSX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67272"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VN041_02125"
     CDS             complement(<1..532)
                     /locus_tag="VN041_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27877.1"
                     /translation="MTTGFSALIVLTGLAFGTLRGAHPFRLSVSPAGLGEGRWWTPLT
                     ALLVPDSVIELVLTLALAIIGLGYAERLLGSLRSAVAFIVTGVLGMLVGVGVQALSMT
                     EGAAWARISGPVLDPAVGVVGATMVASALAPVLWRRRIRLLGFSALIMFALYSGDVDS
                     VYRLCAATVGLVLGAVL"
     gene            complement(616..1923)
                     /locus_tag="VN041_02130"
     CDS             complement(616..1923)
                     /locus_tag="VN041_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWU27878.1"
                     /translation="MLAWLLGLDIISGPAPWVLWGITAALIVVLLVRRYTGGRALRAL
                     LAVVIGGVLGLALSWMLDAIDAFGTPLPDPVKWWAAGCFAAIGLGIVSLWDSTAPRKI
                     VAVVTIIASLLSGTIGINAAFGLDRNLGDLLEISTLQPVGELPKPVRTAAATADAPLY
                     TTWHAPAGMPAHGEVRQLSGASRIPSTAGFVPRDAAIYLPPAALVADPPVLPVMVQMM
                     GLPGSPTPTAIQTAVDAFAAKHDGLGPIVIVADQLGSSRQNPGCADSKAFGGVNTYFN
                     VDIPEWIRTHLRVPAAPADWTISGYSNGGACSFLYGAQHPEIWGSLASQSGEEWPGWE
                     RPAPVLSKVFGGSMAAFDANKPENVLAAHPGAYRGRVAVFTAGAVDHVYGPPARHAAD
                     LARVAGFTTTFFAIAGAGHVGPALSVGLDETLRVLAPVWGLAP"
     gene            2121..2303
                     /locus_tag="VN041_02135"
     CDS             2121..2303
                     /locus_tag="VN041_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HWU27879.1"
                     /translation="MAGKFELYQDKGGDWRFRLKAGNGEVIATGQGYASKSGAVNGID
                     SVRRNAADAEVVEVEN"
     gene            complement(2364..3119)
                     /locus_tag="VN041_02140"
     CDS             complement(2364..3119)
                     /locus_tag="VN041_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWU27880.1"
                     /translation="MSTDLINLSAPAIDIQGLVKHYGDNEVLKGIDLTVTAGEVVCVI
                     GPSGSGKSTLLRSVNMLEDPTGGKIVIEGIDITDPDVDIDRVRARIGMVFQSFNLFPH
                     MSVLGNLTIAQRQVKKRGKAEAEKVAKEMLERVGMGHKADAYPSHLSGGQQQRVAIAR
                     ALCMNPDMMLFDEPTSALDPELVGEVLQVMRSLADEGMTMLVVTHEMGFAREVGSRLI
                     FMDGGHILEQGDPREVLANPQHPRTQDFLSRVL"
     gene            complement(3116..3901)
                     /locus_tag="VN041_02145"
     CDS             complement(3116..3901)
                     /locus_tag="VN041_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWU27881.1"
                     /translation="MKKRTRTRVYHYTVYAVIVALIAWAAASTDWPTVAKQFANPEVA
                     LKMLPGIITVGLKNTLLFTVESFLGGLILAVVLALMKLSSIPPFRWAATVWIEFFRGI
                     PALMTIFGFALMLPIALHVKVPGGPVGAATLGLIVVSSAYMAETIRAGIQAVPKGQTE
                     ASRSLGMSPMKTMFFIVLPQGFRIIIPPLTNEFVLLLKDTSLFFIAGATPLTYELTTY
                     ARNGLSTYANATPMVMAALLYLVVTIPLTRLTAWLERRMARER"
     gene            complement(3928..4752)
                     /locus_tag="VN041_02150"
     CDS             complement(3928..4752)
                     /locus_tag="VN041_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU27882.1"
                     /translation="MNRRPLIAGIAIAAAAVLALSGCASNPGSGGGGGGDKAPSGPDY
                     GLVTAGQITVCSDIPYKPFEYEATSGSGKYTGFDVDLLTAIAKKLDLKVQFQVTNFDT
                     LQSGAALLAGQCDMGASAMTITDERKKNIDFSDGYYDSLQSLLVKTDSGIESIKDLAG
                     KNVGVQQGTTGENYAKEHAKGAELLQYSDDGVLWPALQGGQIDAILQDLPVNLQHEKD
                     DSSYKVVETYKTDEQYGFAFAKGEKAELRKAIDEQLKALKDDGEYKKIYDKYFATN"
     gene            complement(4930..5229)
                     /locus_tag="VN041_02155"
     CDS             complement(4930..5229)
                     /locus_tag="VN041_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HWU27883.1"
                     /translation="MIRHVVSWKLAEPDAALRGEQAAEVARRLNALMGVVPELRAVSA
                     GANVAHPDANWDVTLVADVDSLDALEAYQVHPAHKEAGAYIKSVVAARVAVDFEF"
     gene            complement(5226..5489)
                     /locus_tag="VN041_02160"
     CDS             complement(5226..5489)
                     /locus_tag="VN041_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWU27884.1"
                     /translation="MTTLTLIGKPDCHLCEVASEVVDAVVAELPDAAAEQIEIVERSI
                     NDDPALYERWWEKIPVVLIDGQLHAHWRVSPERLKDALEEAIG"
     gene            complement(5486..5779)
                     /locus_tag="VN041_02165"
     CDS             complement(5486..5779)
                     /locus_tag="VN041_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU27885.1"
                     /translation="MKSIDIAQLAAREGVPLIDVRERDEYAAGHVPGAVNLPLSELGE
                     RLEELPGEAFDVICQAGGRSARAVQALEARGYDATNVEGGTGAWIAEGRPVEQ"
     gene            complement(5846..5944)
                     /locus_tag="VN041_02170"
     CDS             complement(5846..5944)
                     /locus_tag="VN041_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HWU27886.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNKK"
     gene            complement(6062..6271)
                     /locus_tag="VN041_02175"
     CDS             complement(6062..6271)
                     /locus_tag="VN041_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU27887.1"
                     /translation="MPDVQDVRFLTVAEVAELMRVSKMTVYRLVHSGELPAIRFGRSY
                     RVPESAVAEALQRPIADDGPIADVG"
     gene            complement(6383..6799)
                     /locus_tag="VN041_02180"
     CDS             complement(6383..6799)
                     /locus_tag="VN041_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU27888.1"
                     /translation="MTDIFDVIADGTRRDILQLLLSRSTDSDAGTSVTEIVSELGVSQ
                     PTVSKHLKVLRESDLVTVREDGQRRFYSLSVAPLEAVDDWLVPFLVDAYGDQAPDIDF
                     PVMPLPDGAAHAAEVVGRAAASAKHVVASAFKRLGS"
     gene            6904..8334
                     /locus_tag="VN041_02185"
     CDS             6904..8334
                     /locus_tag="VN041_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWU27889.1"
                     /translation="MSGMMSASSPRRSVGDVARQVKHLITSSPSRFAIVIFAALILLF
                     TGILSLPISSADGHVTPLSDALFTAVSTICVTGLTTVDMSTYWSPFGQIVIFIGVNIG
                     GLGVLTLASLMGMVISKRLGLSAKLLAAGDTNPLRAHGGPVNESQTVRLGEVGQLLTT
                     VAMSALIIEGGLAVLLYPAIVMAGVDPVSALWEAPYFAAMSFTNTGFAPNAGGVLAFN
                     DDYLVLLLLMAGVFLGSIGFPVIYTLAKHTWHFKRWSLHSKLTLVTTVLLFFAGAAVF
                     LLLEYDNPRTFGSMDAADTTVHAFFLSAMTRSGGFNAIDMSDLNGSSMLVACMLMFVG
                     GGSASTAGGIKVTTLAVLAIAVWSEAKGRQSVEAFGRRIPSDVQRVGLSVVAWGATIV
                     AVSTVAITQITKLPVIDVLFDVISAFGTVGLSSGVTEALPDAGSYILAATIFMGRVGT
                     VTLAAAVAATSRSQLYSLPVERPIVG"
     gene            8327..8998
                     /locus_tag="VN041_02190"
     CDS             8327..8998
                     /locus_tag="VN041_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWU27890.1"
                     /translation="MVEMVRGDAPVLVIGLGRFGAACAGELDRLDREVMAIDEDLGLV
                     QKWSERVTHTVQADARNIDALRQIGAQDFQVAVVAVGSLIEASVLITANLVDLKVPQI
                     WAKAVSQSHGKILARVGANHVIYPEREAGERVAHLVSGRMLDFIRFDDDFVLAKMYPP
                     KFIRGVGLNESGVRTKYKVTVVGVKSPGKPFRYAEANTVVTNHDLIIVSGTNSDIERF
                     ASLDR"
     gene            9255..10325
                     /locus_tag="VN041_02195"
     CDS             9255..10325
                     /locus_tag="VN041_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU27891.1"
                     /translation="MSAPDSTLFDGIAARVVTTPRLATQILERVGDDPSAPPRRTVVL
                     VHGNVSSALFWQEIMLRLPSDLRVIALDLRGFGGSGSQPVDATRGLRDYSDDIAELLS
                     ELGIVSAHLVGWSMGGGVVMQYALDHRVESLTLQAPVSPYGFGGTRRDGSRLTDDDAG
                     CGGGAANPDFVQRLQDEDTTADAQTSPRSVFRSSYVSAGYSSPHEDVWVASMLSTSTA
                     TGNYPGDSVASENWPGFAAGAHGVLNTMAPQYFNAAGIVDLDEKPPVLWVHGTADAIV
                     SDASFFDLNHLGALGIIPGWPGADIAPAQEMVSQTRDVLDAYAADGGRVTELNWEGVG
                     HSPHLERPDEFCAALMAHIESV"
     gene            complement(10348..11187)
                     /gene="proC"
                     /locus_tag="VN041_02200"
     CDS             complement(10348..11187)
                     /gene="proC"
                     /locus_tag="VN041_02200"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWU27892.1"
                     /translation="MVDSLPSLAFLGAGSMGGAILQGVVASGVPVDGGIVATNRTREK
                     ADALAGLSGVRSISLAENPAGNREAAASARIVLIGVKPVMVPDLLREIAPVLREDAIV
                     VSLAAGVTLDTFADVLGAGVHSIRSMPNTPATVGRAVTGIAAGASATSEDMALVRALF
                     ETVGAVIEVPESQIDPLSTVSGSGPAYVFLLIEELTKAASGMGFSDADARLMAEQTFI
                     GAAALLEASGEDPAELRRRVTSPKGTTERAVAVMQDAHLDDMFARATAAALARAKELA
                     AGA"
     gene            11268..12239
                     /locus_tag="VN041_02205"
     CDS             11268..12239
                     /locus_tag="VN041_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWU27893.1"
                     /translation="MSASGGSKAIVAAFLANMGIALAKFIAWALSGSASMLAEAIHSV
                     ADSGNQLLLMLGGRKAKKDADRDHPFGYGRERYVSAFVVSIVLFSLGGLFAVYEGVQK
                     IVHPHELDKAWWWLPLAVLVISIALESYSLRTAIKESNQVREKGQSWISFVRRSKAPE
                     LPVVLLEDTGALTGLTFALLGVGLTLITGNPLFDALGTLMIGLLLVAIALILGVETKS
                     LLVGEGANRADYDRIVDAISRGPEVEKLIHIKTLYLGPDELMVAAKIALTADKSVRDA
                     ASDIDEIERRIRSAVPAARVIYLEPDVYRPALDPEPPTDTFVLKSSD"
     gene            12248..13141
                     /locus_tag="VN041_02210"
     CDS             12248..13141
                     /locus_tag="VN041_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU27894.1"
                     /translation="MIDDLRAELARTDLPPVTEQKLRKLLAVLEKDSQARLESLAMVD
                     PGPHAVVSFGDVEAADLVIFLLHGIDTDLAHFPEWADAAQRLCVDVIRSCVARGEPRN
                     VAVIAWFAWDSGTHVSALATKHATVGAARLAVDIDRLERRNPRARIALATYSYSSTLL
                     GELFAMNIGEDVHTAFSIASAGVTHAAGVALAEAVARGDLVLYATEGANDSIAPLGRL
                     GQHPVDPRDIPGAIVYECDGGEAPAIGGGFTVGIAVEGHASQTTTDEHGRHIGYYDEH
                     AQGYLTMVARLADAAIGAPAR"
BASE COUNT         2065 a         4557 c         4470 g         2112 t
ORIGIN      
        1 cgagcacggc gccgagcacg agtccgacgg tcgccgcgca cagccggtac acgctgtcga
       61 cgtcgccgct gtacagcgcg aacatgatca gcgccgagaa gccgagcagg cggatgcggc
      121 ggcgccacag caccggagcg agcgcgctcg cgaccatggt cgcgccgacg acaccgaccg
      181 cggggtcgag caccggcccg gagatgcgcg cccaggctgc gccctccgtc atcgagagcg
      241 cctggacgcc gacgcccacg agcatcccga gcacgcctgt cacgatgaac gcgaccgcgc
      301 tgcgcagcga ccccagaagg cgctccgcat atcccaggcc gatgatcgcg agcgccagcg
      361 tcagcaccag ctcgatcacc gagtccggca cgagcagggc ggtcagaggg gtccaccagc
      421 gtccttcacc caggcccgca gggctcaccg agagacggaa cgggtgagcg ccccgcagcg
      481 tgccgaatgc cagtcccgtc agcacgatga gagcagagaa tcctgtcgtc acgggatgag
      541 cgcgcagcgc cccgaaagcc ctgcctctca ggtgcgtggt gctccgcgca tcgcccgtag
      601 cactgctccg tccgctcatg gtgcgagccc ccagaccggc gccagcaccc gcagcgtctc
      661 atccaggccg accgacaggg ccggacccac atgtcctgcc ccggcgatcg cgaagaaggt
      721 cgtcgtgaag ccggcgaccc gcgccaggtc cgcggcatgc cgggccggcg gcccgtagac
      781 gtggtcgacc gcgccggccg tgaacaccgc cacccgcccg cggtacgcgc ccggatgcgc
      841 tgccagcacg ttctcgggct tgttggcgtc gaacgcggcc atgctgccgc cgaagacctt
      901 gctcagcacc ggggcgggtc gctcccagcc cggccactcc tcgccggact ggctcgcgag
      961 cgagccccag atctccggat gctgcgcgcc gtacaggaac gagcatgcac cgccgttgga
     1021 gtacccgctg atcgtccagt cggcaggcgc cgccgggacg cgcaggtgcg tgcggatcca
     1081 ctccgggatg tcgacgttga agtacgtgtt cacaccgccg aaggccttcg agtccgcgca
     1141 cccggggttc tgcctcgacg agccgagctg atcggcgacg atcacgatcg ggccgagacc
     1201 gtcgtgcttc gcggcgaagg cgtcgaccgc ggtctggatc gcagtcgggg tcggactgcc
     1261 cggcaggccc atcatctgca ccatgaccgg aagaacggga ggatccgcca ccagggccgc
     1321 cgggggcagg tagatcgcgg cgtcgcgcgg cacgaagccg gcggtcgacg ggatgcgcga
     1381 ggcaccggag agctgccgca cctcgccatg tgcgggcatg cccgccggag cgtgccaggt
     1441 ggtgtacagc ggcgcgtccg cggtagccgc ggcagtgcga acgggcttcg ggagctcgcc
     1501 gaccggctgg agcgtggaga tctcgagcag atcgcccagg ttccggtcca gcccgaatgc
     1561 ggcgttgatg ccgatcgtgc ccgagagcag cgaggcgatg atcgtcacga ccgcgacgat
     1621 cttgcgcggc gccgtggagt cccagaggct gacgatgccg aggccgatgg ccgcgaagca
     1681 tccggcggcc caccacttga ccgggtcggg cagcggagtg ccgaaggcgt cgatcgcatc
     1741 cagcatccag ctgagggcga gcccgagaac accaccgatg accacggcca gcaaggcccg
     1801 cagcgcccgc cctcctgtgt atcggcgcac cagcagcacg acgatgagcg ccgcggtgat
     1861 cccccacagc acccaggggg cggggccgga gatgatgtcc aggccgagca gccaggcgag
     1921 catccgcctc ccccgttccg cacagtcgca tgccgtgctc gcccatgcta gatccgggtg
     1981 gcttcgcgtc gcccttgaga cgtctatgca ggagccgtgg atcggatcgc gccgcggatt
     2041 ccctcttccc ggccgcgtcc gggtcgagct agctttgtgc cggggcgatg acgcccttac
     2101 ccgtagggag aggaaatctc atggcaggca agttcgagct ctaccaggac aagggcggcg
     2161 actggcggtt ccgcctgaag gccggcaacg gcgaggtcat cgcgaccggg cagggctacg
     2221 cgtcgaagtc cggcgccgtc aacggcatcg actcggtgcg ccgcaacgcc gcggacgccg
     2281 aggtcgtcga agtcgagaac tgagcgcacg cgcgaaagga ggccgtcgac ccgtgggtcg
     2341 gcggcctcct cctcgtcaaa gcgtcagagc acgcgcgaca ggaagtcctg cgtgcgcgga
     2401 tgctgcggat tggccagcac ctcgcgcggg tcgccctgct cgaggatgtg cccgccgtcc
     2461 atgaagatca ggcgggagcc gacctcccgg gcgaagccca tctcgtgcgt gacgaccagc
     2521 atggtcatgc cctcgtcggc gaggctgcgc atcacctgca gcacctcgcc caccagctcc
     2581 gggtcgagcg ccgaggtcgg ctcgtcgaag agcatcatgt cggggttcat gcagagcgca
     2641 cgggcgatcg ccacacgctg ctgctgaccg ccggagaggt ggctcgggta ggcatccgcc
     2701 ttgtgtccca tgccgacgcg ctccagcatc tccttcgcga ccttctccgc ctcggccttg
     2761 ccgcgcttct tgacctgacg ctgagcgatc gtcaggttgc cgagcacgct catgtgcggg
     2821 aacaggttga agctctggaa caccatgccg atgcgggcgc gcacgcgatc gatgtcgacg
     2881 tcgggatcgg tgatgtcgat tccctcgatc acgatctttc cgccggtggg gtcctccagc
     2941 atgttcaccg agcggaggag cgtcgacttt ccagagcccg acgggccgat cacgcagacg
     3001 acctcgccgg ccgtcacagt cagatcgatg cccttgagca cctcgttgtc gccgtagtgc
     3061 ttgaccaggc cctggatgtc gatcgccggg gcactcagat tgatcaggtc ggtactcatc
     3121 gttctctcgc catccgtcgc tccagccacg cggtcagtcg tgtcagcggt atcgtcacga
     3181 ccaggtacag cagcgccgcc atgaccatcg gcgtcgcgtt ggcataggtg gacagtccgt
     3241 tgcgggcgta ggtggtcagc tcatatgtga gcggcgtcgc tcccgcgatg aagaacagcg
     3301 acgtgtcctt cagcagcagc acgaactcgt tcgtgagcgg cgggatgatg atgcggaacc
     3361 cctgaggcag cacgatgaag aacatggtct tcatcggaga catgcccagc gaacgggagg
     3421 cctcggtctg ccccttcggg acagcctgga tgccggcgcg gatcgtctcc gccatgtagg
     3481 ccgacgacac cacgatcagg ccgagcgtcg cggccccgac ggggccgccg gggaccttca
     3541 cgtgcagcgc gatcgggagc atgagcgcga agccgaagat cgtcatcagt gcgggaatac
     3601 cgcggaagaa ctcgatccat acggtcgccg cccatcggaa cggggggatg ctgctgagct
     3661 tcatcaacgc cagcacgacg gcgaggatga ggccgccgag gaaagactcg acggtgaaca
     3721 gcagcgtgtt cttcagtccg acggtgatga tgccgggaag catcttcagt gcgacctcgg
     3781 ggttcgcgaa ctgcttcgcg acggtcggcc agtcggtgga ggccgctgcc catgcgatga
     3841 gcgcgacgat caccgcatag acggtgtagt gatagacccg cgttcgggtc cgcttcttca
     3901 tagccatcgt cttggttcag gcagcgatca gttcgtggcg aagtacttgt cgtagatctt
     3961 cttgtactcg ccgtcgtcct tgagcgcctt cagctgctcg tcgatcgcct tgcgcagctc
     4021 ggccttctcg cccttcgcga acgcgaaacc gtactgctcg tcggtcttgt aggtctcgac
     4081 gaccttgtac gacgaatcgt ccttctcgtg ctgcaggttg accggcaggt cctgcaggat
     4141 cgcgtcgatc tgtccgccct gcagggcggg ccacagcacg ccgtcatccg agtactgcag
     4201 gagttcggcg cccttggcgt gctccttcgc atagttctcg ccggtcgtgc cctgctgcac
     4261 gccgacgttc ttccctgcga ggtccttgat gctctcgatg cctgagtcgg tcttcaccag
     4321 gagcgactgc agcgagtcgt agtacccatc ggagaagtcg atgttcttct tccgctcgtc
     4381 ggtgatggtc atcgccgacg ctcccatgtc gcactggccg gcgagaagcg cggcgccgga
     4441 ctgcagggtg tcgaagttgg tcacctggaa ctggaccttg aggtcgagct tcttcgcgat
     4501 cgcggtgagc aggtcgacat cgaacccggt gtactttccg gagccgctcg tggcctcgta
     4561 ctcgaacggc ttgtagggga tgtcggagca cacggtgatc tgcccggcgg tcaccagacc
     4621 gtagtccggc cccgagggtg ccttgtcgcc gccaccaccg ccacccgagc ccgggttgct
     4681 ggcgcagccg ctcagggcga ggacggcggc agccgcgatc gcgatgccgg cgatgagggg
     4741 acgacggttc atggtcactc ctgtgtgacg gggcttgcgc ccggggacgg gtatggcatt
     4801 catcttgaca tgacctctcc tgcatcgcag aacgcacgaa ggccacgggg atcacggttg
     4861 ggttgcgacc gtgcgcattt ctgcaaggca gtatgaacag atgtcgtcaa ctctgatgag
     4921 ttctgctgat cagaactcga agtcgacggc gacccgggcc gcgaccaccg acttgatgta
     4981 ggcgcccgcc tccttgtggg caggatgcac ctggtaggcc tcaagcgcat cgagcgagtc
     5041 gacgtcggcg accagggtca catcccagtt ggcatccgga tgcgcgacgt tcgcgcccgc
     5101 gctcaccgcg cgcagctcgg gcacgacgcc catcagcgcg ttcagccgcc gggcgacctc
     5161 ggccgcctgt tcgccgcgca gcgcggcatc cggctcggcc agcttccagc tcaccacgtg
     5221 acggatcatc cgatcgcctc ctcgagtgcg tccttgaggc gctcgggcga gacgcgccag
     5281 tgcgcgtgca gctggccgtc gatcagcacc accgggatct tctcccacca gcgctcgtac
     5341 agcgccgggt cgtcgttgat ggaccgctcg acgatctcga tctgctcggc cgcggcatcc
     5401 gggagctctg cgacgaccgc gtcgacgacc tcgctcgcga cctcgcagag atggcagtcc
     5461 ggcttgccga tcagcgtgag ggtggtcact gctcgaccgg acgcccttcg gcgatccacg
     5521 cccccgtgcc gccctcgacg ttggtggcgt cgtagccgcg ggcctcgagt gcctgcacgg
     5581 cgcgggccga gcggccgccg gcctggcaga tcacgtcgaa cgcctcgccg ggcagctcct
     5641 cgagacgctc gccgagctcg gacaggggca ggttgacggc gccgggcacg tggccggccg
     5701 cgtactcgtc gcgctcccgc acatcgatga ggggcactcc ctcacgggcg gcgagctgtg
     5761 cgatgtcgat cgacttcatg gcatccatta tcgcccggaa aacacgaagc gcccgtccgg
     5821 agacaggcgc tcggtgtcgt gccgcttact tcttgttgcg gcgctggtgg cgagtcttgc
     5881 gaagcagctt gcggtgcttc ttcttcgcca tgcgcttgcg gcgcttcttg atgactgaac
     5941 ccacggaaac ctcactaagt caggggctgg atgccacgaa ggcgggcacc cgggtacggg
     6001 cacggaaaat gcctcggatg attctagcaa acggtgggcg tcagcccgaa tcgcgctcgg
     6061 atcatcctac gtcggcaatg ggtccgtcgt cggcgatggg ccgctgcagg gcctcggcga
     6121 cagcggactc cggcacccgg tagctgcgac cgaagcggat cgcgggcagt tcgcccgagt
     6181 gcacgagccg gtacaccgtc atcttggaca cgcgcatgag ctcggcgacc tcggccaccg
     6241 tcaggaatcg aacgtcttgg acatcgggca tcccccgtac ccctttctgc tgaagcacag
     6301 catatggggt acgtgcgacg gctgtaaaca gctgtgactg atgtgaatcc tgtggcggca
     6361 cccgtgaatg ccgttgcggc gctcagctgc cgagacgctt gaacgcggac gccaccacgt
     6421 gcttcgccga cgcggcggcg cgacccacca cctcggccgc gtgcgccgcg ccgtcgggca
     6481 gcggcatgac cgggaagtcg atgtcgggcg cttggtcgcc gtaggcgtcg acgaggaacg
     6541 gcaccagcca gtcgtcgacg gcttcgaggg gggcgacgga caggctgtag aagcggcgct
     6601 gaccgtcctc ccggaccgtg accagatcgg actcgcgcag caccttgagg tgcttggaga
     6661 cggtcggctg gctgacgccg agttcgctga cgatctcggt gacgctcgtg ccggcatcgc
     6721 tgtccgtgga gcggctcagc aggagctgga ggatgtcacg cctcgtgccg tccgcgatca
     6781 cgtcgaagat gtccgtcatg ccatcagggt agtcggcgcg ggcacggagt accatgcgcg
     6841 gccacggagt accatgtcgg aagcccggcg aaggacgtcg tgcgcacagc aggagggggc
     6901 tcgatgtcgg ggatgatgtc ggcttcctct ccgcgacgct ctgtcggcga tgtagcgaga
     6961 caggtgaagc acctcatcac ctcctctccg tcgcgcttcg cgatcgtcat cttcgccgcg
     7021 ctgatcctgc tgttcacggg catcctgtcc ctgccgatct cgtctgccga cgggcacgtc
     7081 accccgctca gcgacgcgct gttcaccgcg gtctcgacca tctgcgtcac gggcctgacg
     7141 acggtcgaca tgagcaccta ctggtccccc ttcgggcaga tcgtcatctt catcggcgtg
     7201 aacatcggcg gcctgggcgt gctgacactg gcgtccctga tgggcatggt gatctccaaa
     7261 cggctgggcc tgagcgccaa gctcctcgct gccggcgaca ccaacccgct gcgcgcccac
     7321 ggcggcccgg tgaacgagag ccagaccgtg cgcctgggcg aggtcgggca gctgctgacc
     7381 accgtcgcga tgtcggcgct gatcatcgag ggcggcctgg cggtgctcct ctatccggcg
     7441 atcgtgatgg caggggtcga ccccgtctcc gcactgtggg aggcccccta tttcgcggcc
     7501 atgtcgttca ccaacacggg cttcgcaccg aacgccggcg gcgtcctcgc gttcaacgac
     7561 gactacctgg tgctgctgct gctgatggcg ggcgtcttcc tcggcagcat cggcttcccg
     7621 gtgatctaca cgctcgccaa gcacacctgg cacttcaagc gctggtcgct gcactccaag
     7681 ctcacgctcg tcaccaccgt gctgctgttc ttcgcgggcg ccgccgtgtt cctactgctg
     7741 gagtacgaca acccgcgcac cttcgggtcg atggatgccg ccgacaccac cgtgcacgcg
     7801 ttcttcctct ccgcgatgac ccgctcgggc ggattcaacg cgatcgacat gtccgatctg
     7861 aacggctcgt cgatgctcgt ggcctgcatg ctgatgttcg tcggcggcgg ttcggcgtcg
     7921 acggccggcg gcatcaaggt gaccacgctc gccgtgctcg cgatcgccgt gtggtcggag
     7981 gccaagggcc ggcagtcggt cgaggccttc gggcggcgca tcccctccga cgtgcagcgg
     8041 gtcggactgt cggtcgtggc ctggggggcg accatcgtgg cggtctcgac ggtcgcgatc
     8101 acgcagatca ccaagctgcc ggtgatcgac gtgctcttcg acgtgatctc cgcgttcggc
     8161 acggtcggcc tctcctccgg ggtcacggag gcccttccgg acgccggctc ctacatcctc
     8221 gcggcgacca tcttcatggg gcgcgttggt acagtgactc tcgccgcggc ggtcgccgcg
     8281 acgtcccgtt cgcagctcta ctcgctgccg gtggaaaggc cgatcgttgg ttgagatggt
     8341 tcgcggcgac gcgccggtgc tcgtgatcgg actcgggcgc ttcggcgccg cctgcgcggg
     8401 cgagctcgac cgcctcgacc gcgaggtgat ggcgatcgac gaggacctcg gcctcgtgca
     8461 gaagtggtcc gagcgcgtca cccacaccgt gcaggcggat gcccgcaaca tcgacgcgct
     8521 gcggcagatc ggcgcgcagg acttccaggt ggccgtcgtc gccgtcggct cgctgatcga
     8581 ggcatccgtc ctcatcaccg ccaacctcgt cgacctgaag gtgccgcaga tctgggcgaa
     8641 ggccgtctcg cagtcgcacg gcaagatcct cgcccgcgtc ggcgccaacc atgtgatcta
     8701 ccccgagcgc gaggccggcg agcgcgtcgc gcacctcgtc agcgggcgga tgctggactt
     8761 catccgcttc gacgacgact tcgtgctcgc caagatgtac ccgccgaagt tcattcgcgg
     8821 cgtgggcctg aacgaatcgg gcgtgcgcac caagtacaag gtgaccgtgg tgggtgtgaa
     8881 gagcccgggc aagccgttcc ggtacgccga ggcgaacacc gtggtcacca accacgacct
     8941 catcatcgtc tcgggcacga acagcgacat cgagcggttc gcgagcctcg accgctgagc
     9001 cgggcgggct ggtcgccgag agccgcgcag ccatgtcgca gaactggcgg gtgcagtgcg
     9061 cccgcaccgg ccacgacgga gacatcctcg tgggctcggc tcagaacggg cgggtccgag
     9121 ccgcatgaac ccgccactgc tgtgacactc ccgtccaacc gaacctgaaa gccagttcac
     9181 attctggtcc ttcgcgggtt agagtcgtgc cagcacgcgg cgcagaggcc gcgtcacggc
     9241 acgaaggagc ggcgatgagc gcccccgaca gcaccctctt cgacggcatc gcagcccgcg
     9301 tcgtcaccac tccgcgactg gccacgcaga tcctggagcg cgtcggcgac gacccgtccg
     9361 ctcccccgcg ccgcaccgtc gtgctcgtgc acggcaatgt gtcgtcggcg ctgttctggc
     9421 aggagatcat gctgcggctc ccgtccgacc tgcgcgtgat cgcgctcgac ctgcgcggct
     9481 tcggcggcag cggctcgcag ccggtggacg ccacacgcgg cctgcgcgac tacagcgacg
     9541 acatcgcgga gctgctgtcc gagctgggca tcgtctcggc gcacctggtg ggttggtcga
     9601 tgggcggcgg cgtggtcatg cagtacgcgc tcgaccaccg cgtggagagc ctcacgctgc
     9661 aggcgccggt ctcgccctac ggcttcggcg gcacccgccg cgacggctca cggctgacgg
     9721 acgacgacgc cggatgcggt ggcggcgccg ccaaccccga cttcgtgcag cgcctgcagg
     9781 acgaggacac caccgccgac gcacagacgt cgccgcgcag cgtgttccgg tccagctacg
     9841 tgtccgcggg gtactcgtcg ccgcatgagg acgtctgggt ggcatccatg ctcagcacgt
     9901 cgacggcgac gggcaactac ccgggcgact cggtcgcgag cgagaactgg cctggcttcg
     9961 cggcgggtgc gcacggcgtg ctgaacacga tggcaccgca gtacttcaat gcggccggca
    10021 tcgtcgacct cgacgagaag ccgccggtgc tgtgggtgca cggcacggcg gatgccatcg
    10081 tctcggacgc ctcgttcttc gacctcaacc acctgggcgc actggggatc atccccgggt
    10141 ggcccggtgc cgacatcgcg ccggcgcagg agatggtctc gcagacccgc gacgtgctcg
    10201 acgcatacgc agcggacggc ggacgggtca ccgagctgaa ctgggagggc gtcggccact
    10261 ccccgcacct ggagcgcccg gacgagttct gtgcggcgct gatggcgcac atcgagagcg
    10321 tctgagcgca gcatccgctc tcggaagtca ggcgccggcg gcgagctcct tcgcgcgggc
    10381 gagcgcggcc gcggtcgccc gcgcgaacat gtcgtcgagg tgcgcgtcct gcatcacggc
    10441 gacggcgcgc tcggtcgtcc ccttggggct ggtcacgcga cggcgcaact cggcggggtc
    10501 ctcgccggac gcctcgagca gggccgccgc gccgatgaag gtctgctcgg ccatgaggcg
    10561 ggcatccgca tcggagaagc ccatccccga cgccgccttc gtcagctcct cgatcaggag
    10621 gaacacgtag gcgggcccgg accccgagac cgtggagagc gggtcgatct gcgactcggg
    10681 cacctcgatc accgcgccga ccgtctcgaa caacgcccgt acgagggcca tgtcctctga
    10741 ggtggcggag gctccggcgg cgatgccggt caccgcacgc cccaccgtcg ccggcgtgtt
    10801 cggcatggag cggatgctgt gcactccggc gcccaggaca tccgcgaacg tgtccagcgt
    10861 cacaccggcc gccaggctca cgacgatcgc gtcctcgcgc agcaccgggg cgatctcgcg
    10921 cagcaggtcg ggcaccatca ccggcttcac gccgatcagc acgatgcgcg cggaagcggc
    10981 ggcctccctg ttgcccgcgg ggttctcggc cagcgagatg ctgcgcacgc cggagagtcc
    11041 ggccagggca tccgccttct cgcgggtgcg gttcgtggcg acgatgccgc cgtcgaccgg
    11101 gactcccgag gcgacgacgc cctgcaggat cgcccctccc atcgagccgg caccgaggaa
    11161 cgcgagggag ggaagcgaat cgaccatgcc gcccatccta cgagccgtcg tcctgccacc
    11221 gggcgtgtgc ccggaaccgt actgcgctca cgtcttagac tcgtggcatg agcgcatccg
    11281 gtggcagcaa ggcgatcgtc gcggcattcc tggcgaacat gggcatcgcc ctcgccaagt
    11341 tcatcgcgtg ggcgctctcc ggctcggcct cgatgctcgc cgaggcgatc cactccgtcg
    11401 ccgactccgg caaccagctg ctgctcatgc tcggcgggcg caaggccaag aaggacgcgg
    11461 atcgcgacca ccccttcggc tacggccgcg agcgctacgt gtcggcgttc gtggtgtcga
    11521 tcgtgctgtt ctcactcggc ggtctgttcg ccgtgtacga gggcgtgcag aagatcgtcc
    11581 atccgcacga gctcgacaag gcctggtggt ggctgccgct cgccgtgctc gtgatctcga
    11641 tcgcgctgga gtcgtactcg ctgcgcaccg cgatcaagga gagcaatcag gtgcgcgaga
    11701 agggccagtc ctggatctcc ttcgtgcgcc gctccaaggc gccggagctg cccgtcgtgc
    11761 tgctcgagga caccggggcg ctcacgggcc tcaccttcgc cctgctgggc gtggggctga
    11821 cgctgatcac cggcaacccc ctcttcgacg cgctcggcac gctgatgatc ggtctgctgc
    11881 tggtggcgat cgcactgatc ctgggtgtgg agaccaagag cctgctggtc ggcgagggcg
    11941 cgaaccgcgc ggactacgac cggatcgtgg atgccatctc ccgcggcccc gaggtggaga
    12001 agctcatcca catcaagacg ctctacctcg gacccgacga gctgatggtc gcggccaaga
    12061 tcgcgctgac cgcagacaag tcggtccgcg acgcagcatc cgacatcgat gagatcgagc
    12121 ggcggatccg ttccgccgtt cctgcggcgc gcgtgatcta cctggagccg gacgtctacc
    12181 gtcccgccct cgacccggag ccgccgacgg acacgttcgt actgaagtcc tccgactgac
    12241 gcacgtcgtg atcgacgacc tccgcgccga gctggcgcgc accgacctgc ccccggtgac
    12301 ggagcagaag ctgcgcaagc tgctggccgt gctggagaag gactcgcagg cacgactgga
    12361 atccctcgcg atggtcgacc ccggcccgca tgcggtcgtc tcgttcggcg acgtcgaggc
    12421 ggccgatctg gtgatcttcc tgctgcacgg aatcgacacc gatctggccc atttcccgga
    12481 gtgggcggat gccgcgcaac ggctgtgcgt cgatgtcatc cgctcctgcg tggcgcgcgg
    12541 cgagccgagg aacgtggccg tcatcgcctg gttcgcctgg gattcgggca cgcacgtctc
    12601 ggcactggcg accaagcacg ccacggtggg cgcggcgcga ctggcggtgg acatcgaccg
    12661 cctggagcgg aggaatcccc gcgcccgcat cgcgctggcg acctactcgt actcgtcgac
    12721 gctcctgggc gagctgttcg ccatgaacat cggcgaggac gtgcacaccg cgttctccat
    12781 cgcctcagcc ggcgtgacgc acgccgccgg tgtcgcactc gcagaggccg tcgcccgcgg
    12841 ggacctggtg ctctacgcca ccgagggagc gaacgacagc atcgccccgc tgggtcgtct
    12901 cgggcagcat cccgtggatc ctcgcgacat cccgggggcg atcgtctacg agtgcgacgg
    12961 aggcgaggcg cccgcgatcg gcggcggctt caccgtcgga atcgccgtcg agggccacgc
    13021 gtcgcagacc acgaccgacg agcacggccg ccacatcggg tactacgacg agcacgcgca
    13081 ggggtatctc acgatggtcg cgcggctcgc ggatgctgcg atcggcgcgc ccgcgcgctg
    13141 attccgcgga cgcttcacgc ggagaagtgc gcttcgcgcg ggacggatcc ccgcgtccgt
    13201 ccgc
//
LOCUS       DATMSX010000070         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180986,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000070 DATMSX010000000
VERSION     DATMSX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180986"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1870
                     /gene="hutU"
                     /locus_tag="VN041_02215"
     CDS             191..1870
                     /gene="hutU"
                     /locus_tag="VN041_02215"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWU27895.1"
                     /translation="MSEPTLHDPTRSIRAARGNQRTAKSWGAEAAKRMLMNNLDAEVA
                     EHPEDLVVYGGTGRAARSWEAYDAIVRTLDELEPDETLLVQSGKPVGVFRTHEWAPRV
                     LIANSNLVGDWATWPEFRKLEAEGLMMYGQMTAGSWIYIGSQGILQGTYETFAAVAQS
                     LGKESLKGTLSLTGGAGGMGGAQPLAVTLNEGACLIVDVDETRLARRVDHGYLDVYYT
                     DLDEAVARAVAAKEAGEALSVGIVGNAAEVFPELFRRQQAGEIAIDVVTDQTSAHDPL
                     AYLPIGISVEDWKAEAERDPEEFTRRSREAMAAHVAAMVAFQDAGAAVFDYGNSIRAE
                     AKLGGFDRAFEFPGFVPAYIRPQFEEGRGPFRWAALSGDPEDIYKTDRAIAELFPEDK
                     ALHRWLEKAGEKVHFEGLPARICWLGYKERHLAGLKFNEMVASGELSAPIVIGRDHLD
                     SGSVASPYRETEAMKDGSDAIADWPLLNALLNTSSGASWVSLHHGGGVGIGRSIHAGQ
                     VTVADGSELAAEKLERVLTNDPGTGVMRHVDAGYEHARDIARERGLKVPML"
     gene            1867..>2190
                     /locus_tag="VN041_02220"
     CDS             1867..>2190
                     /locus_tag="VN041_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU27896.1"
                     /translation="MSTTLITNIGELTTNVGSPLDRSGTLTDAAVLIEGDRIAWVGPA
                     SDVPALPGGYLEVDAEGRAVIPGFVDSHSHIVFGGDRAAEFEARMAGEKYAAGGIRST
                     VAATRG"
BASE COUNT          343 a          820 c          731 g          296 t
ORIGIN      
        1 cctcgagatg tggcgcgagc gcctggcgca ccgcgagcca tgtcggcccc gggtttgaaa
       61 caaacgcatc gtccagccgg atcgcgcaca accacgcccg gacccgacca cttcctctca
      121 cccgacatgg aggccgtcac cgagctcgtg cgctcgggca ccgtcctccg cactgcgaag
      181 gagcacaccg atgtctgagc ccaccctcca cgacccgacc cgcagcatcc gcgctgcgcg
      241 cggcaaccag cgcaccgcca agagctgggg tgccgaggcc gccaagcgga tgctgatgaa
      301 caacctcgac gccgaggtcg ccgagcaccc cgaggacctc gtcgtctacg gcggcaccgg
      361 tcgcgcggcc cgcagctggg aggcgtacga cgcgatcgtg cgcaccctcg acgagctcga
      421 gcccgacgag accctgctgg tgcagtccgg caagccggtc ggcgtgttcc gcacccacga
      481 gtgggcgccg cgcgtgctga tcgccaactc caacctcgtc ggcgactggg ccacctggcc
      541 cgagttccgc aagctcgagg ccgagggcct gatgatgtac ggccagatga cagccggttc
      601 gtggatctac atcggctcgc agggcatcct gcagggcacc tacgagacgt tcgccgctgt
      661 tgcgcagtcg ctgggcaagg agtccctgaa gggcaccctg tcgctcaccg gcggggccgg
      721 cggcatgggc ggtgcgcagc cgctggccgt caccctcaac gagggcgcct gcctcatcgt
      781 cgacgtggac gagacccgtc tcgcccgccg agtcgaccat ggctacctcg acgtctacta
      841 caccgacctc gatgaggccg tggcccgtgc ggtcgccgcc aaggaggcgg gcgaggccct
      901 ctccgtcggc atcgtcggca acgccgccga ggtcttcccg gagctgttcc gccgccagca
      961 ggccggcgag atcgcgatcg acgtcgtgac cgaccagacc agcgcgcacg acccgctcgc
     1021 gtacctgccc atcggcatct ccgtcgagga ctggaaggcc gaggccgaac gcgaccccga
     1081 ggagttcacc cgtcgctcgc gcgaggcgat ggccgcgcac gtcgccgcca tggtcgcgtt
     1141 ccaggacgcc ggggccgccg tgttcgacta cggcaactcc atccgcgccg aggcgaagct
     1201 cggtggcttc gaccgggcgt tcgagttccc cggcttcgtg ccggcgtaca tccgcccgca
     1261 gttcgaagag ggccgcggcc cgttccgctg ggctgccctc tccggcgacc cggaggacat
     1321 ctacaagacc gaccgcgcca tcgcggagct gttccccgag gacaaggcgc tgcaccgctg
     1381 gctggagaag gccggcgaga aggtgcactt cgagggtctg cccgcacgca tctgctggct
     1441 gggctacaag gagcgccacc tggccggtct gaagttcaac gagatggtcg cctcaggcga
     1501 gctgtccgcc ccgatcgtga tcggccgaga ccacctggac tccggttcgg tggcgagccc
     1561 gtaccgcgag accgaggcca tgaaggacgg ctccgacgcg atcgccgact ggccgctgct
     1621 gaacgccctg ctgaacacct cgtcgggcgc cagctgggtg tcgctgcacc acggcggcgg
     1681 cgtcggcatc ggccgctcga tccacgccgg ccaggtcact gtcgccgacg gctccgagct
     1741 cgccgccgag aagctcgagc gcgtgctgac caacgacccc ggcaccggcg tcatgcgtca
     1801 cgtggacgcc ggctacgagc acgcccgcga catcgcccgc gagcgcggcc tcaaggtgcc
     1861 gatgctgtga gcaccacgct catcacgaac atcggggagc tgacgaccaa cgtcggctcc
     1921 ccgctggacc gttccggaac gctcacggac gcggccgtcc tcatcgaggg cgaccgcatc
     1981 gcgtgggtcg gcccggcttc ggacgtcccc gcacttcccg gcggctacct cgaggtcgac
     2041 gccgaaggcc gtgcggtcat ccccggcttc gtcgacagtc acagccacat agtgttcggc
     2101 ggcgaccgcg cggccgagtt cgaggcgcgc atggccggcg agaagtacgc ggccggcggc
     2161 atccgctcca ccgtcgccgc gacccgcggc
//
LOCUS       DATMSX010000071         7521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_95759,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000071 DATMSX010000000
VERSION     DATMSX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95759"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VN041_02225"
     CDS             complement(<1..701)
                     /locus_tag="VN041_02225"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWU27897.1"
                     /translation="MSMLIEEGRTEAMAAARFAGPTLQLIRGAVAENLRRMRTGGPVM
                     AVVKADGYGHGALAVASAAVDAGAEWLGVTDVAEGALLRGSGIETPILAWLNPAGIDV
                     VAAVRNRIDVAVGSVDELAELIDDALVAGPARGGARVRVHLHLDTGMARGGCPVRDWD
                     RMLQLARRGRDAGLLRIVGVMGHLPQADLADPRANVPWVMRLRHARDTALRAGLAPLI
                     AHLGATSAALSDPSRS"
     gene            complement(698..1348)
                     /locus_tag="VN041_02230"
     CDS             complement(698..1348)
                     /locus_tag="VN041_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWU27898.1"
                     /translation="MNSSAAEARRNRRAAALFATLVVALIAAVFVVQQALTGASASTS
                     PPPQAGQVQQEVPVKQAAADDGRITAGDGLIAEGRPAGVDDNVPAVTNLDPPLLAALR
                     RAAAAASADGVELRINSGWRSREFQDRLLADAVKKYGSAQEAARWVATADASEHVSGD
                     AADIGPWAAAEWLGRHGAAFGLCQIYGNEPWHFELRAKASTEGCPRMLRDPTERRR"
     gene            1437..2102
                     /locus_tag="VN041_02235"
     CDS             1437..2102
                     /locus_tag="VN041_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU27899.1"
                     /translation="MRVLIVEDEPYLAEAIRDGLRLEAIAADIAGDGDTALELLSINS
                     YDLAVLDRDIPGPSGDEVAAWIVASGSGIPIMMLTAADRLDDKVSGFELGADDYLTKP
                     FELRELVMRLRALDRRRVHARPPVREIAGLRLDPFRREVFRDGKYVALTRKQFAVLEV
                     LVGAEGGVVSAEELLERAWDENADPFTNAVRITVSALRKRLGEPWVIATVPGVGYRID
                     ADA"
     gene            2099..3229
                     /locus_tag="VN041_02240"
     CDS             2099..3229
                     /locus_tag="VN041_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU27900.1"
                     /translation="MTSVSPRRRGLSARMKLTLSYAGSFGMAGALIVAAGWTLQLLRS
                     FIPTLDALLHTAIPWQLRQFLADYLIPGSVGIILLLLAIGFLWGWFVAGRMLAPLERI
                     GEIARLAAQGDLSRRVALEGRGDEFRDLADVFDMMLDRIEAQVAEQQRFAANASHELR
                     TPLAIMRTQLEVAQADPDRDTDALLARLHQVNGHAIDLTEALLLLARADGQAFDRVPV
                     DLSLLAEEAVETLLPLAERRGIVIESATDPAFVVGSPTLLQQLITNLLHNAIVHNVPE
                     NGRIGLRISTTETSVLLAVANTGPHIPDALLATLAEPFQRGAGRARRTDTQPLDAPSE
                     QRGLGLGLAIAQSIVRAHGALLRLTARPEGGLLVEAWLPRAR"
     gene            complement(3445..4965)
                     /locus_tag="VN041_02245"
     CDS             complement(3445..4965)
                     /locus_tag="VN041_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU27901.1"
                     /translation="MTDAPDASEPLAPSGIDPDELAVALKVLAELDQVDQQHPDYIAV
                     RRATGKMFRAVKRARRKEIRDAIAEADRAVVARTATGAPDRIDDETRGLDLATSVVDA
                     PIAGELLKPRNCYICKKPYTLVDAFYHQLCPDCARFSHAKRDAHADLTGKRALLTGGR
                     AKIGMHIALRLLRDGAHLTITTRFPRDAVRRFSSLPDSADWLHRLRVVGIDLRDPAQV
                     IALADSVAAQGPLDILINNAAQTVRRSPGAYSLLADAELQPLPNGPLPEMETFGHTAD
                     PHPQALQASVDAHPLLSVAALGGTVAEQGGQALTAEDLARLAMAPGSSSLEKHSDGTA
                     IDAGGLVPDINRVNSWVQSVDQVDPLEMLEVQLCNTTAPFLLISRLRPAMAASTARRK
                     YIVNVSAMEGQFSRRYKGPGHPHTNMAKAALNMLTRTSAGEMLETDGILMTAVDTGWI
                     TDERPHYTKVRLAEEGFHAPLDLVDGAARVYDPIVQGEAGVDLHGVFLKDYEPSPW"
     gene            5046..6158
                     /locus_tag="VN041_02250"
     CDS             5046..6158
                     /locus_tag="VN041_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HWU27902.1"
                     /translation="MSTTGVAEESIHQGIVPGYLLARLARSSQFTHAAEAARQTLIKG
                     RPEYRATLELSVDSNGDLIADVQAAPSRTIYDTHNTEQLPGDKVRGEDDPAVKDAAVN
                     QAFDGLGDTFRMLLGAFQRDSLDGGGAPLTATVHYGEKYDNAFWNGSQMVFGDGDGEV
                     FTGFTESVTVIGHELGHGVIQTTAALEYQGQSGALNESCADVFGALTEQYAKDQSADQ
                     ASWLIGEGIFTPAVQGRALRDMLHPGTAYDDDELGKDPQPADMSHYVRTTEDNGGVHI
                     NSGIPNRAFALAATAIGGKAWETAGLVWYRTLTGGLSATADFTEFAQATITAAADIGA
                     SVETAVRDAWIAVGVVEGGVVEGGVIKDASADRAPE"
     gene            6130..6471
                     /locus_tag="VN041_02255"
     CDS             6130..6471
                     /locus_tag="VN041_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HWU27903.1"
                     /translation="MPQQIEPPSDGSDATDDADRIVIAVVRTGGIAGLRRRWRVEPDP
                     EQTPEWAALVERCPWDEHPDPSAETGADRYVWSIHAVIHDTQHEQVLPDALLHGAWRE
                     LVDAVRAADTD"
     gene            6508..7389
                     /locus_tag="VN041_02260"
     CDS             6508..7389
                     /locus_tag="VN041_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU27904.1"
                     /translation="MTQKIVPNIWFDRNAGEAGEFYAAALPKTKTHVAMRYPDDLPAS
                     QAGMEGQPVVVDVEVDGFHLTLINSDDTYRPTPAISFILNFDPLMFDGDPDAARAALD
                     ATWDALSDGGAALMPLGEYPFSPRFGWVADRYGVSWQLMLTRPEGDPRPFVIPQIMFA
                     NDVEGKAREAAELYTSLLPDAGVGTVIPRPTGGVMFGEFRVGDQWFSIMDSDIPHQFG
                     FTPGVSLEVRCEDQAEIDRLWDALSAVPEAEACGWLVDRFGVSWQIVPANIGELLSRP
                     GGYQRMLGMKKLIIADF"
BASE COUNT         1084 a         2639 c         2672 g         1126 t
ORIGIN      
        1 gatctggacg ggtcgctcag cgccgccgag gtcgcaccga gatgcgcgat cagcggagcc
       61 agacccgcgc gcagagcggt gtcgcgggcg tgccgcagac gcatcaccca cggcacgttc
      121 gcccgaggat cggccaggtc cgcctgcggc agatggccca tcaccccgac gatgcgcaga
      181 agcccggcat cccgtccacg gcgtgcgagc tgcagcatcc gatcccagtc ccgcacgggg
      241 cagccgcccc gcgccatccc tgtgtccagg tgcagatgca cccgcacccg agcaccaccg
      301 cgcgccggac cggccaccag agcgtcgtcg atcagctcgg cgagctcgtc caccgacccg
      361 actgcgacgt cgatccggtt ccgcacggcg gcgacgacgt cgatccccgc cggattcaac
      421 caggcgagga tcggcgtctc gatcccgctc ccgcgcagca gagctccctc ggccacatcc
      481 gtgacgccca gccactcggc ccccgcatcg acggcggcac tcgccacggc gagcgcgccg
      541 tgcccgtacc cgtcggcctt gaccacggcc atgacgggcc ctccggtgcg catccgccgc
      601 aggttctcgg cgacggcgcc ccggatcagc tgcagcgtgg gcccggcgaa acgcgcggcg
      661 gccatcgctt ccgtccgtcc ctcctcgatc agcatgctca tcgcctccgc tccgtgggat
      721 cgcgcagcat ccgcggacac ccctcggtgg acgccttcgc gcgcagctcg aagtgccagg
      781 gctcgttgcc gtagatctgg cacagcccga aggccgcacc gtggcgtccc agccattcgg
      841 ccgccgccca cgggccgatg tcggcggcat cgccggagac gtgctcggag gcgtcggcgg
      901 tggccaccca gcgcgcggcc tcctgcgccg agccgtactt cttcacggcg tcggccagca
      961 gccggtcctg gaactcccgc gaccgccagc cgctgttgat cctcagctcg accccgtctg
     1021 cggatgccgc ggcggcggcg cgacgcagtg ccgcgagcaa cggggggtcg aggttggtca
     1081 cggcgggcac attgtcgtcc acgcccgccg gcctcccctc ggcgatcagc ccgtcgcccg
     1141 ccgtgatgcg cccgtcgtcg gcagcggcct gcttcaccgg cacctcctgc tgcacctgcc
     1201 ccgcctgcgg aggcgggctg gtggaggcgg atgccccggt cagggcctgc tgcaccacga
     1261 agaccgcggc gatcagcgcg accaccaggg tggcgaaaag agcggcggcc cgtcggttgc
     1321 gtcgggcctc ggcggcagat gagttcatgc tgaccagtcc acgcgcgtgc ccgttgcgag
     1381 gacgtatgcg cttttcgata tgttcccgat atgcgccggt gcgtaggatc cttcacatgc
     1441 gcgtgctgat cgtcgaggac gagccctatc tcgcggaggc catccgcgac gggctccgtc
     1501 tcgaggcgat agccgccgac atcgccggcg acggcgacac cgcactcgag ctgctgagca
     1561 tcaactccta tgacctggcg gtgctcgacc gggacatccc cgggccctcc ggcgacgagg
     1621 tggcggcgtg gatcgtggca tccggcagcg gcatcccgat catgatgctg accgccgctg
     1681 atcgtctcga cgacaaggtg tccggcttcg agctgggtgc cgacgactat ctcaccaagc
     1741 cgttcgagct gcgtgagctg gtcatgcggc tgcgggcgct cgatcggcgc cgtgtgcacg
     1801 cgaggccgcc cgtgcgcgag atcgcggggc tgcgtctgga cccgttccgg cgtgaggtct
     1861 tccgggatgg gaagtacgtg gcgctcacgc gcaagcagtt cgcggtgctg gaggtgctcg
     1921 tgggcgccga aggcggcgtg gtgagcgcgg aggagctgct ggagcgcgcc tgggacgaga
     1981 acgccgaccc gttcaccaac gccgttcgga tcacggtctc ggcgctgcgc aagcgtctcg
     2041 gcgagccctg ggtgatcgcg acggttcccg gcgtcggcta ccggatcgac gcggacgcat
     2101 gacctccgtg tcccctcgcc gccgcggtct cagcgcgcgg atgaagctga cgctcagcta
     2161 cgccgggtcg ttcggcatgg cgggcgcgct catcgtggcc gcgggctgga cgctgcagct
     2221 gctgcgcagc ttcatcccca ccctggacgc gctgttgcac acggcgatcc cgtggcagct
     2281 gaggcagttc ctggccgact atctgatccc ggggtccgtg gggatcatcc tcctgctgct
     2341 cgcgatcggc ttcctgtggg gctggttcgt cgccggccgg atgctggcgc cgctcgagcg
     2401 catcggggag atcgcccgtc tcgccgcgca gggagacctc tcgcgccggg tcgccctgga
     2461 gggcaggggc gacgagttcc gcgacctggc cgacgtcttc gacatgatgc tcgaccgcat
     2521 cgaggcgcag gtggccgagc agcagcggtt cgcggccaac gcctcgcacg agctgcgcac
     2581 gccgctggcg atcatgcgca cccagctgga ggtggcgcag gcggacccgg atcgggatac
     2641 ggacgcactg ctggcccggc tgcaccaggt gaacgggcat gcgatcgatc tgaccgaggc
     2701 tctgctgctg ctcgcgcgcg ccgacgggca ggcgttcgat cgcgtgccgg tggatctgtc
     2761 gctgctcgcc gaggaggcgg tggagacgct gctgcctctg gccgagcgtc gcgggatcgt
     2821 gatcgagtcg gccaccgacc ccgcgttcgt ggtgggctcg ccgaccctgc ttcagcagtt
     2881 gatcacgaac ctgctgcaca acgcgatcgt gcacaacgtg cccgagaacg ggcgcatcgg
     2941 gctgcggatc tcgacgacgg agacgtcggt gctgctcgcg gtcgccaaca ccggtccgca
     3001 catcccggat gccctgctgg cgacgctggc ggagccgttc cagcgcggcg cgggccgggc
     3061 tcggcgcacg gacacgcagc ccctcgacgc cccgagcgag cagcgcggcc tgggtctcgg
     3121 actggccatc gcgcagagca tcgtccgtgc gcatggcgcg ctgctgcgcc tcaccgcccg
     3181 tcccgagggc ggtctgctcg tggaggcctg gctgccgcgg gcgcggtgac gcgcagcatc
     3241 cgctgtccac ggccccgcct cggtgccggc cggctccacg cgggtacaga tgtcggtcga
     3301 aaacacggat gccggtccag aatccgcttt ccccaccgac aaacgggatc cgcgccgaca
     3361 tctgtaccgg gctgggcggc gggatccgga tgccgtgtgg acggatgcca tgcacccggg
     3421 cgcccgccgc tcaccagggc tggctcacca ggggctgggc tcgtagtcct tcaggaagac
     3481 gccgtgcaga tccacgccgg cctcgccctg cacgatcggg tcgtacacgc gggcagcgcc
     3541 gtcgaccagg tcgagcgggg cgtggaatcc ctcttcggcc agacgcacct tcgtgtagtg
     3601 cgggcgctcg tccgtgatcc agccggtgtc gacggcggtc atcaggatgc cgtcggtctc
     3661 gagcatctca ccggcgctcg tgcgcgtgag catgttcagc gcagccttgg ccatgttggt
     3721 gtgcgggtgc ccaggaccct tgtaccggcg ggagaactgc ccctccatgg ccgacacgtt
     3781 cacgatgtac ttgcgccgag cggtcgatgc ggccatggcc ggtcgcagtc ggctgatcag
     3841 caggaagggc gccgtcgtgt tgcagagctg cacctcgagc atctccagcg gatcgacctg
     3901 gtcgaccgac tgcacccagc tgttcacgcg gttgatgtcg ggcacgagcc cgccggcgtc
     3961 gatcgcggtg ccgtcggagt gcttctccag cgacgacgac ccgggcgcca tggcgagccg
     4021 ggcgaggtcc tcggcggtca gcgcctgccc gccctgctcg gcgacggtgc caccgagggc
     4081 cgcgaccgag agcagcggat gcgcatcgac cgacgcctgc agcgcctgcg gatgcgggtc
     4141 agcggtgtgc ccgaaggtct ccatctcggg cagcggcccg ttcgggagtg gctgcagctc
     4201 ggcatccgcc agcagcgagt acgcacccgg cgagcgccgc acggtctgtg cggcattgtt
     4261 gatgaggatg tcgagcggcc cctgggccgc gaccgagtcg gccagcgcga tcacctgggc
     4321 ggggtcgcgc aggtcgatgc cgaccacccg cagccggtgc agccagtcgg cggagtcggg
     4381 cagagacgag aagcggcgca ccgcgtcgcg cgggaagcgc gtcgtgatcg tgaggtgggc
     4441 cccgtcgcgc agcagccgca gtgcgatgtg catgccgatc ttggcgcggc cgccggtcag
     4501 cagtgcgcgc ttgccggtca ggtcggcgtg ggcgtcgcgc ttggcgtggc tgaaccgcgc
     4561 gcagtcgggg cagagctggt ggtagaaggc gtcgacgagc gtgtacggct tcttgcagat
     4621 gtagcagttg cggggcttga gcagctcgcc ggcgatcggg gcgtcgacca cgctcgtggc
     4681 gaggtcgagc ccgcgggtct cgtcgtcgat gcggtcggga gcgccggtcg cggtgcgggc
     4741 gaccaccgcc ctgtcggcct cggcgatggc gtcccggatc tccttgcggc gcgcacgctt
     4801 gacggcgcgg aacatcttgc cggtggcccg gcggacggcg atgtagtccg gatgctgctg
     4861 atcgacctgg tcgagctcgg cgagcacctt cagcgcgacg gcgagttcat cggggtcgat
     4921 gccggagggc gcgagcggct cggaagcatc aggggcatcg gtcattgggc cgattctacc
     4981 ggccggacct gggcgctccg gagtggggct gaccgttgtc ggaaccccgg cgtatcgtcg
     5041 gccgtatgag caccaccgga gttgcagaag agtccatcca tcaggggatc gtcccgggct
     5101 acctgctcgc gcgactcgcc cgttcgagcc agttcacgca cgcggccgaa gccgcacggc
     5161 agaccctgat caaggggcgc ccggagtacc gcgccacgct cgagctctcg gtcgactcca
     5221 acggcgacct gatcgcggat gtgcaggccg ccccctcgcg gaccatctac gacacccaca
     5281 acaccgagca gctccccggc gacaaggtgc gcggcgagga tgacccggcg gtgaaggatg
     5341 ccgcggtgaa ccaggccttc gacgggctgg gcgacacctt ccggatgctg ctgggagcgt
     5401 tccagcgcga ctcgctcgac ggcggcggcg ccccgctgac cgccaccgtg cactacggcg
     5461 agaagtacga caacgcgttc tggaacggct cccagatggt gttcggcgac ggcgacggcg
     5521 aggtgttcac gggcttcacc gagtcggtca ctgtcatcgg gcacgagctc ggccacggcg
     5581 tgatccagac gaccgccgcg ctggagtacc agggccagtc cggggcgctg aacgagtcgt
     5641 gcgccgacgt gttcggggcg ctcaccgagc agtacgccaa ggatcagagc gccgaccagg
     5701 cgagctggct gatcggcgag ggcatcttca ccccggccgt gcagggccgg gccctgcgcg
     5761 acatgctgca ccccggcacc gcctacgacg acgacgagct gggcaaggac ccgcagccgg
     5821 ccgacatgtc gcactacgtg cggaccaccg aggacaacgg cggcgtgcac atcaactccg
     5881 gcatcccgaa ccgcgccttc gcactggcgg ccacggcgat cggcggcaag gcctgggaga
     5941 cggcaggtct ggtctggtac cgcaccctca ccggcgggct gtctgcgacc gccgacttca
     6001 cagagttcgc gcaggcgacc atcaccgctg ccgccgacat cggggctagc gtggagacgg
     6061 cggtgcgcga cgcgtggatc gccgtcggag tggttgaagg cggagtggtt gaaggcggag
     6121 tgatcaagga tgcctcagca gatcgagccc ccgagtgacg gatcggatgc cacagacgat
     6181 gcggaccgca tcgtcatcgc cgtggtgcgc accggcggga tcgccgggct ccgccgccgc
     6241 tggcgcgtcg agcccgatcc ggagcagacc ccggagtggg ccgccctggt cgagcgctgc
     6301 ccctgggacg agcatccgga cccctccgcg gagaccggtg ccgatcgcta cgtctggagc
     6361 atccacgccg tcatccacga cacgcagcac gagcaggtgc tgccggatgc cctgctgcac
     6421 ggcgcctggc gtgagctggt cgatgccgtc cgcgccgcag acaccgactg aaccccgatt
     6481 cccgcccccg ctgaaggaca ggaccccatg actcagaaga tcgttcccaa catctggttc
     6541 gaccggaacg caggcgaggc gggtgagttc tacgccgcag cgctgccgaa gaccaagacg
     6601 cacgtcgcca tgcgctaccc ggacgatctg cccgcctcgc aggcgggcat ggaagggcag
     6661 ccggtggtgg tcgacgtcga ggtcgacggc ttccacctca cgctgatcaa ctcggacgac
     6721 acgtatcggc ccacccccgc gatctcgttc atcctgaact tcgatccgct gatgttcgac
     6781 ggtgacccgg atgccgctcg cgcggcgctc gatgcgacct gggacgcgct gtccgacggc
     6841 ggcgcagcgc tcatgccgct cggcgagtac ccgttcagcc cgcggttcgg gtgggtggcc
     6901 gaccggtacg gcgtgagctg gcagctgatg ctgacccggc ccgagggcga cccgcgaccg
     6961 ttcgtgatcc cgcagatcat gttcgcgaac gacgtcgagg gcaaggcgcg tgaggcagcc
     7021 gagctgtaca cgtcgctgct gccggatgcc ggtgtcggca ccgtgatccc ccgtccgacc
     7081 ggcggcgtga tgttcggaga gttccgtgtc ggggaccagt ggttctcgat catggactcc
     7141 gacatcccgc accagttcgg gttcaccccc ggggtctcgc tcgaggtgcg ctgcgaggac
     7201 caggccgaga tcgatcggct gtgggatgcc ctgtcggccg tgccggaggc ggaggcctgc
     7261 ggttggctgg tcgaccggtt cggggtgagc tggcagatcg tgcccgcgaa catcggcgag
     7321 ctgctgtcga gaccgggcgg ctaccagcgg atgctcggca tgaagaagct gatcatcgcc
     7381 gacttctgat ccggttgtct atcctgatcg agtgaagcga ctcaccgccg cctcggctct
     7441 gaccggactc ctcctgctca ccggatgctc cggcacgccc gccccggcct cggccggcgc
     7501 cgctgcatcc ggagcgccgt c
//
LOCUS       DATMSX010000072         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_19811,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000072 DATMSX010000000
VERSION     DATMSX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19811"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VN041_02265"
     CDS             <1..1387
                     /locus_tag="VN041_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWU27905.1"
                     /translation="PPDATAVAALGRLLPRRSFMKLKAESLTNDGTALVLYAIAVSLA
                     VGGQVTPLIVAWTVVVSYLGGILVGAAVAAAGWMLLRRTPSTIAINVTMLLVPFVSFL
                     LAEMIHASGVLAVVVAGLIAAHLSSRVTTAASRRQAEAVWPFGVFLLNGALFVLIGLE
                     VQWVAHQTSLQTIGWLLLATVVVWVTLLVVRFLFQLLALPFQRLGGGQVHPRGLRTRM
                     QIVSTVAGMRGAVSLAIALSVPIADGEGIRLAGRDDIIFVTAGVILLSLLVQGPLLPA
                     VVRWARFPVDNGEAEEYELAERAISGAAMAALDGLATEHGISDEVRDRLRRDGYERLE
                     LANARAMARERALIDAEVGAMEVLLDSPDPLADGSVELSDQAGAAGPSSDRVTLQMVA
                     TSTEVDLEQRSPLIRHEEMSRLKLAVIEHKREVLRGLRRSGTVDDLVVYRISADLDLE
                     QVRLQGIEQLD"
     gene            complement(1425..2126)
                     /locus_tag="VN041_02270"
     CDS             complement(1425..2126)
                     /locus_tag="VN041_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU27906.1"
                     /translation="MNADDTTFLWARPGPAKRGPKPRFTLDAIADAAVAIADEHGLEA
                     VTMQEVASRLGTTKMALYRYLPGRAELDALILDHALDSPGPLSGGGWRSALARWAEEL
                     HRRMRARPWAVELSQRPHVPGPVELTWFEGALSEMADLPLRGSEKLDVLALLAGHVMS
                     LARRQAQVERPEADLARGLAPILSAHAGEFPRTASAFSETADDGTQDDALRFGVDRIL
                     DGIQVLVTERGSPDV"
     gene            2210..3382
                     /locus_tag="VN041_02275"
     CDS             2210..3382
                     /locus_tag="VN041_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU27907.1"
                     /translation="MKVLISGASIAGPALARWLGRNGFDVTVVEKATAPRPGGQAVDF
                     KGRTHRRVLERMGVLEDVLAHQTAKTDWRFVDENDRVKATVPGEFIGGDLEILRGDLA
                     GILHEHSRADAEYVFDDEIVDLAESDSGVDVAFARRKGERFDLVIGADGVHSVVRRLA
                     FGAEELFVQARDRYYAVAGGEMPLDGLERELPGGRAIAYGYNEPGRLALLGGQKAPSL
                     FVFHAAAPDYDRRDVASQRAFLESAFAGAGWRVPEAIAAGRDADDFYLDALARTTMTS
                     FTRGRVTLVGDAGFANTLGGFGTGLALIGAYVLAGELIAARGDWAAALAAYDRRMRKP
                     TRIARSGDAGSFLAPPSRWRIRARDWSFANPVMYRSMLWLTNAFATDDSVPDYRMD"
     gene            complement(3384..>3957)
                     /locus_tag="VN041_02280"
     CDS             complement(3384..>3957)
                     /locus_tag="VN041_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWU27908.1"
                     /translation="RSAARRIAELLPEDRVVVSDGGHFIGWANMYWPVAAPDRMMMVG
                     TAFQSIGQGWPSVVGATRARPETTVVLTSGDGGGLMAIPDLESAVRAAGGHGMAVIWN
                     DAAYGAEVNLYGLKGLAEGPMLIPQVDFAAFGEAVGAEGVVVRTLDDLERLASWASED
                     PASRRFLVLDLRISGKVIAPYQQEIIRVNS"
BASE COUNT          558 a         1343 c         1439 g          617 t
ORIGIN      
        1 gcctccggac gccaccgcgg tcgccgcgct cggccgcctg ctgccgcggc gcagcttcat
       61 gaagctcaag gccgagagcc tgaccaacga cggcacggcg ctggtgctct acgccatcgc
      121 ggtgtcgctg gctgtcggcg gtcaggtgac gccgctcatc gtcgcctgga cggtggtcgt
      181 ctcctacctg ggcggcatcc tggtcggagc cgcagtcgcc gcggccgggt ggatgctgct
      241 gcgccgtacg ccgtcgacga tcgcgatcaa cgtgacgatg ctgctcgtgc cgttcgtctc
      301 gttcctgctc gccgagatga tccacgcctc gggcgttctc gcggtcgtgg tcgcgggact
      361 catcgccgcc cacctgagct cccgggtcac caccgcggca tcccggcggc aggccgaggc
      421 ggtgtggccg ttcggggtct tcctgctcaa cggcgcgctg ttcgtgctga tcgggctgga
      481 ggtgcagtgg gtggcgcacc agacatcgct gcagaccatc ggctggctcc tgctggcgac
      541 cgtcgtggtc tgggtgacgc tgctcgtggt gcgcttcctg ttccagctgc tcgccctgcc
      601 gttccagcgg ctcggcggag ggcaggtgca tccgaggggc cttcgcacca gaatgcagat
      661 cgtctcgacc gtcgcgggca tgcgcggcgc ggtgtcgctg gcgatcgcgc tgtcggttcc
      721 gatcgccgac ggcgagggca tccgcctcgc cggacgcgac gacatcatct tcgtcaccgc
      781 cggagtgatc ctgctcagcc tgctcgtgca gggcccgctg ctgccggcgg tcgtgcggtg
      841 ggcccgattc cccgtcgaca acggagaggc ggaggagtac gagctcgccg agcgcgccat
      901 ctccggcgcg gcgatggctg cgctcgacgg gctggccacg gagcacggga tcagcgacga
      961 ggtgcgcgac cgcctgcgcc gggacggcta cgagcgcctc gaactcgcca acgcgcgcgc
     1021 gatggccagg gagcgcgccc tgatcgacgc cgaggtgggc gcgatggagg ttctgctgga
     1081 ctctcccgac ccgctcgccg acggctccgt cgagctcagc gaccaggccg gggcggcggg
     1141 gccgagcagc gaccgagtga cgctgcagat ggtcgccaca tcgaccgagg tcgatctgga
     1201 gcagcgctcg ccgctgatcc gccacgagga gatgtcccgg ctcaagctcg ccgtgatcga
     1261 gcacaagcgc gaggtgctgc ggggtctgcg cagatcggga acggtcgacg atctggtcgt
     1321 gtaccggatc tccgccgacc tggatcttga gcaggtgcgg ctgcagggga tcgagcagct
     1381 ggactgaggg gtcccccggt cgtcgaccgc catcgcgcgg gcgctcacac gtcgggggag
     1441 ccgcgctcgg tgacgagaac ctggattccg tcgaggatgc gatcgacccc gaaacggaga
     1501 gcgtcgtcct gagtcccgtc gtcggcggtc tcggagaatg cggacgcggt ccgcgggaat
     1561 tcaccggcgt gcgcgctgag gatgggcgcg agtccgcggg ccaggtccgc ctcgggcctt
     1621 tcgacctgcg cctggcgacg tgcgagcgac atcacgtggc ccgcgagcaa tgccagcacg
     1681 tcgagcttct cgctgccgcg cagcggaaga tccgccatct cggacagcgc gccctcgaac
     1741 caggtgagct cgacggggcc cgggacatgc ggacgctggc tgagctcgac cgcccacggg
     1801 cgggcccgca tgcgtcgatg cagttcctcc gcccatcgtg cgagcgctga tcgccagccg
     1861 ccgccggaca ggggtccggg ggagtcgagt gcatggtcga ggatcagcgc atcgagttcg
     1921 gcacggccgg gcaggtaccg gtacagggcc atcttcgtgg tgccgagccg gctcgcgacc
     1981 tcctgcatcg tcacggcttc caggccgtgc tcgtcggcga tggcgaccgc ggcatcggcg
     2041 atggcatcca gagtgaaccg gggcttcgga ccgcgcttgg cgggcccggg gcgagcccag
     2101 aggaaggtgg tgtcgtcggc gttcatgaaa ctcccggagt ttattgcgtc taatgtacgc
     2161 gattatgtat tgtttccagc ggacgcagtt acgtttcgaa ggagacatca tgaaggttct
     2221 gatctcaggt gcgagcatcg cagggccggc gctcgcgcgc tggctcggac gcaacgggtt
     2281 cgacgtcacc gtcgtcgaga aggcgacggc tccgcgaccc ggcggccagg ccgtggactt
     2341 caagggacgc acgcatcgtc gtgtcctgga gcgcatgggg gtcctcgagg atgtcctcgc
     2401 tcaccagacc gcgaagaccg actggaggtt cgtcgacgag aacgaccggg tgaaggcgac
     2461 cgtgcccggc gagttcatcg gcggcgacct ggagatcctg cgcggcgatc tcgcgggcat
     2521 cctgcatgag cattcgcgtg ccgatgccga gtacgtcttc gacgacgaga tcgtcgatct
     2581 cgccgagtcg gactcgggtg tcgacgtggc cttcgccaga cggaagggcg agcggttcga
     2641 tctcgtgatc ggcgccgacg gcgtgcattc ggtggtgcgg agactcgcgt tcggagccga
     2701 ggagctgttc gtgcaggcgc gcgaccgcta ctacgccgtc gccggcggcg agatgccgct
     2761 cgacgggctc gaacgagagc ttccgggcgg acgcgcgatc gcgtacggct acaacgagcc
     2821 gggccgtctg gctctgctcg gcgggcagaa ggcccccagc ctcttcgtct tccacgccgc
     2881 cgcacccgac tacgaccggc gcgatgtcgc ctcgcagcgc gcattcctgg agtcggcgtt
     2941 cgccggggcc ggctggcggg ttccggaggc gatcgcggcg ggccgtgatg cggacgactt
     3001 ctatctggat gcgctggcgc gcacgaccat gacctcgttc acacgcggac gagtcacgct
     3061 ggtcggcgac gccggcttcg ccaacactct cggaggattc ggtacggggc tggcgctgat
     3121 cggggcgtac gtgctggccg gtgagctgat cgccgcgcga ggggactggg cggcggcgct
     3181 cgccgcatac gaccgccgga tgcgcaagcc gaccaggatc gcccggtccg gcgacgcggg
     3241 gtcgttcctc gcgcccccgt cgcggtggcg catccgcgca cgggactgga gcttcgcgaa
     3301 ccccgtgatg taccgctcca tgctgtggct gacgaacgcg ttcgccaccg acgacagcgt
     3361 gcccgactac cgcatggact gactcaggag ttcacgcgga tgatctcctg ctggtacggg
     3421 gcgatcacct tgcccgagat gcgcaggtcg agcaccagga agcggcggct cgccgggtcc
     3481 tcggatgccc acgacgccag gcgctcgagg tcgtcgagcg tgcgcaccac gacgccctcg
     3541 gcgccgacgg cctcgccgaa cgcggcgaag tcgacctgcg ggatgagcat gggaccctcg
     3601 gccaggccct tcagcccgta gaggttgacc tccgcgccgt aggcggcgtc gttccagatc
     3661 acggccatcc cgtgcccgcc ggccgcgcgg accgccgact ccaggtcggg gatcgccatc
     3721 aggccgccac cgtcgccgga ggtgagcacg accgtggtct ccgggcgcgc gcgggtcgcg
     3781 ccgaccacgc tcggccagcc ctggccgatc gactggaacg cggtgccgac catcatcatc
     3841 cggtcgggtg ccgcgacggg ccagtacatg ttggcccagc cgatgaagtg cccgccgtcc
     3901 gagacgacca cgcggtcctc gggcagcagc tccgcgatgc ggcgcgccgc cgagcgc
//
LOCUS       DATMSX010000073         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_86263,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000073 DATMSX010000000
VERSION     DATMSX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86263"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VN041_02285"
     CDS             <1..1048
                     /locus_tag="VN041_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWU27909.1"
                     /translation="LSLDDVRRLVKRAEILLEQDRLDAREEERRASRSLTFFERDGML
                     HLSLVTDVESGAPIRTAIDGYVTAQFQARKDAEAAAVAARRADGGVAGGGVGADREDG
                     AVSGAESDSLAGGTAFSAATAPGAGCVDEDRRTVAMMRADALTLFCGHVIGCETSVPV
                     AGATVIVRVDLDDLESGTGHGTVDGTDQPVSVAAVRRMAAAGGVIPCVLGSDGEILDL
                     GRERRLFTRAQKFALTERDGGCARCGLPPHMTKVHHINWWKRDTGPTDLSNGVLLCES
                     CHHRIHDNGWDIRIDNPPGNHTRTNQPAGRDTRRHRGTGVNVHAKVWFIPPTHIDPTR
                     TPQLGGRARYDIAA"
     gene            1430..1780
                     /locus_tag="VN041_02290"
     CDS             1430..1780
                     /locus_tag="VN041_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU27910.1"
                     /translation="MRLSRAVPILTVADVPAAVAGYREALGLDVLMDHGWIVTLGNSG
                     AAPQFSLMDRDRTAPVNPTASLEVDDVDDAYQRVADLGLEIVHPLTDEPWGVRRFFFR
                     DADGNIINVLAHRG"
     gene            1832..2311
                     /locus_tag="VN041_02295"
     CDS             1832..2311
                     /locus_tag="VN041_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWU27911.1"
                     /translation="MSPAAAVITVSDRSARGERADASGPIGVAALRAAGFDCDDAIVI
                     PDGADEVERALRETLQRGIRLIVTTGGTGIGPRDRTPEGTARVLDRELPGIAEELRRR
                     GAAEKPAALLSRGLAGVSGNALIVNLPGSTAAVTDGIRLVLEIAHHALGQLEGEDHR"
     gene            2308..2733
                     /locus_tag="VN041_02300"
     CDS             2308..2733
                     /locus_tag="VN041_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWU27912.1"
                     /translation="MTIRLAQISTEPLDVDAHRAAVDDPRMGAEITFVGRVRDNDEDA
                     ATPVVALEYSAHPDAEKTLRTLIAEAVGETDAIVAASHRIGRLEVGEAAVIVSVASGH
                     RGVAYDVSRTLIEAIKHSLPIWKRQIEADGATVWKGLGG"
     gene            complement(2768..3001)
                     /locus_tag="VN041_02305"
     CDS             complement(2768..3001)
                     /locus_tag="VN041_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWU27913.1"
                     /translation="MARVRFFAAAEEAVGSSELTVDAATLAALTASLRDDHPALAEIL
                     PRCSVLVDGARVDDSWPLTGDAVVDVLPPFAGG"
BASE COUNT          502 a         1121 c         1017 g          423 t
ORIGIN      
        1 actgtccctg gatgatgtgc gccggctcgt caaacgagcc gagatcctgc tggagcagga
       61 caggctcgac gcacgtgagg aggagcgtcg ggcgtcccgg tcgctgacct tcttcgaacg
      121 cgacgggatg ctgcacctga gccttgtcac cgacgtggag tccggtgcgc cgatccgcac
      181 cgcgatcgac ggatacgtca ccgcccagtt ccaggcacgg aaggacgccg aggccgcggc
      241 ggtcgctgca cgccgggccg atggcggggt cgcgggtggg ggcgtcggtg cagatcgtga
      301 agacggggcc gtgtcgggtg ccgagagcga ctcccttgcg ggcgggactg ccttctcggc
      361 tgctaccgct ccgggcgcgg gttgtgtcga tgaggaccgg cgcacggtcg cgatgatgcg
      421 cgccgacgcg ctgaccctgt tctgcgggca cgtgatcggc tgcgagacat ctgtccccgt
      481 cgcaggcgcg accgtgatcg tcagggtcga cctcgacgac ctcgagagcg gcaccgggca
      541 cggcacggtt gatgggaccg accagcccgt cagcgtggcc gcggtccggc ggatggcagc
      601 cgccggtggt gtcatcccct gcgtactcgg ctccgatggt gagatcctcg acctcggacg
      661 cgaacgacgc ctgttcaccc gggcccagaa attcgccctc accgaacgcg acggcgggtg
      721 cgcccgatgc ggactcccac cccacatgac caaggtccac cacatcaact ggtggaaacg
      781 cgacaccggg ccgaccgatc tctccaacgg ggtcctgctc tgcgaatcct gccaccaccg
      841 catccacgac aacggatggg acatcagaat cgacaaccca cccggaaacc acacccgcac
      901 gaaccaaccc gccggccgcg atacgcgtcg gcatcgcggt accggagtca acgtgcacgc
      961 gaaagtctgg ttcatcccac ccacccacat cgaccccaca cgcaccccac aactcggcgg
     1021 acgcgcccgc tacgacatcg ccgcctgacc cgaaggagca cgacgcgggg gcgctgagtc
     1081 tgtcgaagtg ccccaggcag gtccgaccct tcgacaagct cagaggccca gtatccgctc
     1141 aggcaccagc atccgcccgg gaccagtacc tgctcgggac ccagcatccg ctcggggacc
     1201 agtacctgct cggggcccag catctgctga ggggctcatc atcttgggtt gctgagcctg
     1261 tcgaagcacc cgctgcgcga acgtccgccg ttcggcaagc gcggtgtgga ctgtggactc
     1321 ggcgctcctg acacgagtat ccatatcggc cgttcgacag gctcaggggc ccaggggcga
     1381 ggcgggcccg ccggtcttcg gacagaggcc ggatggtatc ttcgcgcgca tgagactctc
     1441 ccgcgcagtg cccattctca cggtcgccga cgtgcccgcg gccgtggcgg gctaccgcga
     1501 ggcgctcggt ctcgacgtgc tgatggatca cgggtggatc gtcacgctcg gaaacagcgg
     1561 tgcggcgcca cagttcagcc tgatggaccg cgaccggacc gcgccggtga accccacggc
     1621 atcgctggag gtcgacgatg tggacgacgc gtatcagcgg gtcgctgatc tcggactgga
     1681 gatcgtgcat cccctcaccg acgagccctg gggtgtacga cgcttcttct tccgcgacgc
     1741 cgacggcaac atcatcaacg tgctcgcgca tcgcggctga ccccgtctgt gccgaagtcg
     1801 gtgcgacccg caccgtcgat aggctgaagg catgtcgccg gcagccgcag tcatcacggt
     1861 ctcggaccgc tccgcccgcg gtgagcgcgc agacgcatcc gggcccatcg gcgtcgcggc
     1921 gctccgcgcg gccggcttcg actgcgacga cgcgatcgtc atcccggacg gcgccgacga
     1981 ggtcgaacgg gcactgcgcg agaccctgca gcgcggcatc cgtctgatcg tcaccaccgg
     2041 cggcaccggc atcggcccgc gtgaccgcac ccccgaggga accgcccgcg tgctcgaccg
     2101 cgaactgccg ggcatcgccg aggagctgcg ccgccgaggc gcagccgaga agcccgcggc
     2161 cctgctctcc cgcggactcg cgggcgtcag cgggaacgcg ctcatcgtca acctccccgg
     2221 ttcgaccgcg gccgtcaccg acggcatccg actcgtgctc gagatcgcac atcacgcgct
     2281 cgggcagctc gaaggggagg accatcgatg accatccgcc ttgcgcagat cagcaccgag
     2341 ccgctcgacg tcgacgccca ccgtgccgcc gtcgacgacc ctcgcatggg cgccgagatc
     2401 accttcgtcg gccgcgtccg cgacaatgac gaggacgccg ccaccccggt cgtcgcgctg
     2461 gagtactcgg cccacccgga tgccgagaag accctgcgca cgctgatcgc cgaagccgtc
     2521 ggcgagacgg acgccatcgt cgctgccagc caccgcatcg ggcgcctgga ggtcggcgaa
     2581 gcggccgtca tcgtgtcggt cgcatcgggc caccgcggcg tcgcctacga cgtgtcccgc
     2641 acgctgatcg aggccatcaa gcacagcctg cccatctgga agcggcagat cgaagccgac
     2701 ggcgccacgg tctggaaggg cctcggcggc tgaccctccg tctcgctgcg ctcaggagac
     2761 gcgggactca accgccggcg aacggcggca gcacatcgac cactgcgtcg ccggtcagcg
     2821 gccacgagtc gtccacccgc gcgccgtcga ccagcactga gcagcgcggc aggatctccg
     2881 cgagagccgg gtgatcgtcg cgcaggcttg cagtcagtgc ggcgagagtc gcggcatcca
     2941 cggtcagctc ggagctgccg acggcctcct cggccgcggc gaagaaccgc acccgtgcca
     3001 cgtcaggcct gccagtcgcc ggcgagcttc gtcacgtccg ccacagccgc gcggatgtcg
     3061 tcc
//
LOCUS       DATMSX010000074         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_76764,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000074 DATMSX010000000
VERSION     DATMSX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76764"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VN041_02310"
     rRNA            <1..674
                     /locus_tag="VN041_02310"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            746..822
                     /locus_tag="VN041_02315"
     tRNA            746..822
                     /locus_tag="VN041_02315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:780..782,aa:Ile,seq:gat)
     gene            850..925
                     /locus_tag="VN041_02320"
     tRNA            850..925
                     /locus_tag="VN041_02320"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:883..885,aa:Ala,seq:tgc)
     gene            1231..>1542
                     /locus_tag="VN041_02325"
     rRNA            1231..>1542
                     /locus_tag="VN041_02325"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          413 a          344 c          441 g          344 t
ORIGIN      
        1 acgcgtgaag ttgaccgcct ggggagtacg gccgcaaggt tgaaactcaa aggaattgac
       61 ggggacccgc acaagcggtg gatgatgtgg tttaattcga tgcaacgcga aaaaccttac
      121 ccacctttga catgtacgga agtgaccaga gatggacatg tgctcgaaag agaaccgtaa
      181 cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
      241 cgagcgcaac ccttgccatt agttgctacg aaagggcact ctaatgggac tgccggtgac
      301 aaactggagg aaggtgggga tgacgtcaag tcctcatggc ccttataggt ggggctacac
      361 acgtcataca atggctggta caaagggttg ccaacccgcg agggggagct aatcccataa
      421 agccagtcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg aatcgctagt
      481 aatcgtggat cagaatgtca cggtgaatac gttcccgggt cttgtacaca ccgcccgtca
      541 caccatggga gcgggttctg ccagaagtag ttagcctaac cgcaaggagg gcgattacca
      601 cggcagggtt cgtgactggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct
      661 ggatcacctc ctttctggaa accgcaatcc aaatcaaacg tccacactta tcggttgttg
      721 gaagacaagt cgaattcttc gacatgggtc tgtagctcag ctggttagag caccgtcttg
      781 ataaggcggg ggtcgttggt tcgagcccaa ctagacccac caaacttcca atatcagtgt
      841 ggagatcctg ggggattagc tcagctggga gagcacctgc tttgcaagca gggggtcgtc
      901 ggttcgatcc cgtcatcctc caccaagatt tttatcgtgt catcaacacc aaagcggcct
      961 tgtgagaatg aggctgtttt gttgttggtc aacattgttt gatcaatcgg ctgttcttta
     1021 aaaattcata gagtcgaatc agcgttgtcg acggaaagag caaattcgta aaggttttag
     1081 ctcaccgtgc cgtcggcaac attttttgat tgcgtcaaat gaacttcagc ttgctgcaaa
     1141 gcaaattgaa gacggcataa cgcgtcaggt gaaagacctg acaattcctt gattgactgg
     1201 gatttctcgt gagagacgtc aaagttatag ggtcaagtga ataagagcat gtggtggatg
     1261 ccttggcgat gataggcgac gaaggacgtg atagcctgcg ataagcttcg gggagctggc
     1321 aaataagctt tgatccggag atttctgaat ggggaaaccc acccgcaagg gtatcgcata
     1381 ctgaatacat aggtatgcga agcgaacctg gagaactgaa acatctaagt acccagagga
     1441 aaagacatca accgagattc cgaaagtagt ggcgagcgaa atcggaacag ccttctagtg
     1501 atagtcagac ggttaacaaa acggaatgga aagtccggcc at
//
LOCUS       DATMSX010000075         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_76774,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000075 DATMSX010000000
VERSION     DATMSX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76774"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VN041_02330"
     CDS             <1..485
                     /locus_tag="VN041_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWU27914.1"
                     /translation="ARTVLIHGGSGGVGSFAVQIAHARGARVIATASEANHDYLRSLG
                     AHPVTYGEGLAERVLALNGGPVDAVADFAGGMLDVTLTVLADDGAHVSIADPSVAQHG
                     GRNIWVRPDGEQTARLAAMAAEGVLKVEIAKTFPLEGVADAFRASQTHHVRGKLVIVP
                     "
     gene            600..848
                     /locus_tag="VN041_02335"
     CDS             600..848
                     /locus_tag="VN041_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27915.1"
                     /translation="MSDDFMLHQVSPDDWMIIDMRFPGGDPHHLVACIIEEDGHAEVR
                     WFRHVTLPSRFRSRTDALEALVTAEQPEPAAVLAAAPA"
     gene            complement(915..1175)
                     /locus_tag="VN041_02340"
     CDS             complement(915..1175)
                     /locus_tag="VN041_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HWU27916.1"
                     /translation="MTIGTGIVLFVIGAILAFALNVQVPWADLHMIGYILMGAGVVAF
                     LVGIVLMARRRRTDVVTRTDGTVDGGNVSRRSVREPGSDEVF"
     gene            complement(1301..2764)
                     /locus_tag="VN041_02345"
     CDS             complement(1301..2764)
                     /locus_tag="VN041_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWU27917.1"
                     /translation="MSEASAVPVRLAAEAHPAGTEFVDAANTPETRGIEIIDDSERHG
                     RARDLFFVWSAPGISVLNLTVGASLTLMLGLEIWQALLVLLASSVLAVFPGIVAISGP
                     AAGTSGSVIQRAIYGIHGNKIVIAFYGWFISGVFLALNWVAASFIGAELLMRWGFPDH
                     TAALIVVAVVTAAITVLVAVYGHALILRAYSGIAIGLTVIFALVIGFLWPTIDWGFTQ
                     PEPLTGLPLWASITIAFALLSSSPLSFSNSADMARYLPRETKASSIIGATALGNVFPA
                     VLITAFGVVLGGTVGPDALANGIEYALLEKLPLWLAPLLVLGVIVNTIALNGMTTYTA
                     SMALQSIGVPIRRIPSAVLIGALGCAFTIVLVLSSSLIEAVNLMLQFLLIISVPTMGV
                     YVADIVLRRNRYDGLELFDERPGATFWYRGGFGMAGVVAVVVGGVATALFLSTTVWVG
                     PLGAALGGLDLSVPVGLIVSVGLYALLARRTIRAQIA"
     gene            complement(2847..>3706)
                     /locus_tag="VN041_02350"
     CDS             complement(2847..>3706)
                     /locus_tag="VN041_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU27918.1"
                     /translation="TGDPAADLAQVERAVQLRDELAATGTPWLKVVGVKFILDGVIDA
                     CTAAMVRPYADGSNADPIWSREAATPVAIAADRAGLQLAMHAIGDAASALAVDLVEAC
                     EQANGVGTGRRPRIEHLESVAPETVARMREHGIAASMQPVHCDPAILDNWMAVLGDQR
                     AEDGFPWNWFRDVEVPVALGTDAPTAPHEALHNLFIAMSGRSSIDSALPPYHPERAFT
                     AAQALAAATSGAAYVGAIDDAYGRIAPGFHANVAVLDIDPLSAPTEDLLTGAVLLTLV
                     HGEAAYRRP"
BASE COUNT          604 a         1268 c         1325 g          509 t
ORIGIN      
        1 acgcgcgcac cgtgctgatc cacggcggct ccggcggtgt cgggagcttc gccgtgcaga
       61 tcgcgcacgc ccgcggcgcg cgggtgatcg ccacggcatc cgaggccaac cacgactacc
      121 tgcgcagtct cggcgcgcat cccgtcacct acggcgaggg gctcgcggag cgggtcctcg
      181 ccctcaacgg cggtcccgtc gacgcggtcg ccgacttcgc cggcgggatg ctggacgtga
      241 ccctcactgt gctcgccgac gacggcgcgc acgtgtcgat cgccgacccg tccgtcgcgc
      301 agcacggcgg acggaacatc tgggtgcgcc cggacggcga gcagacggcg cggctcgcgg
      361 ccatggcggc ggagggcgtg ctgaaggtgg agatcgcgaa gaccttcccg ctcgagggcg
      421 tcgcggatgc cttccgcgcc agccagacgc accacgtgcg cggaaagctc gtcatcgtcc
      481 cctgatcggc gtccgatccg actatcagac gccgctgatc agagtcaatc cccgctcgtt
      541 cacagcatcg gtggcgcaca ctgagcagaa tcgggggatc agatgctcag gaggatgcga
      601 tgtcggacga tttcatgctg caccaggtct cgcccgatga ctggatgatc atcgacatgc
      661 gcttccccgg cggcgacccg caccacctcg tggcctgcat catcgaggag gacggccacg
      721 ccgaggtgcg ctggttccgg cacgtcacac tgccctcgcg attccgcagc cgcaccgatg
      781 cgctggaggc tctcgtcacc gccgagcagc ccgagcccgc agcggtcctc gcggcggcgc
      841 ctgcctgacg cccgaagact ccgtttcgcg cacggaaaga cggccgggtc gtatgacccg
      901 gccgtccgtt ccggtcagaa cacctcgtcg ctgcccggct cacgcaccga ccgacgcgag
      961 acgttgccgc cgtcgacggt tccgtccgtg cgcgtgacga cgtccgtgcg ccggcgacgc
     1021 gccatcagca cgatgccgac gaggaacgcg acgacacccg cgcccatcag gatgtaaccg
     1081 atcatgtgga gatccgccca cgggacctgc acgttcaagg cgaaggcgag gatcgctccg
     1141 atcacgaaca ggacgattcc ggtaccgatg gtcatgacgg ttcccttcgt tcaggtggcg
     1201 tcgggtcgtc gccactgcac tcacggtgac cgtgtccgcc cgcgttctga agggggttga
     1261 caaccccgcc gcggccgcgg gccctgcgcg catcccgcga tcaggcgatc tgcgcgcgga
     1321 tcgtcctgcg ggcgagcagc gcgtacagcc ccacggagac gatcagcccc acggggaccg
     1381 acagatccag cccgcccagc gcggctccca gcggtccgac ccacaccgtg gtggacagga
     1441 acagcgcggt agcgacgccg ccgaccacca cggccacgac accggccatg ccgaagccgc
     1501 cgcgatacca gaacgtggcg ccaggacgct cgtcgaacag ctcgaggccg tcgtagcggt
     1561 tgcggcgcag cacgatgtcg gccacgtaga cgcccatggt cggcaccgag atgatcagga
     1621 ggaactgcag catgaggttg accgcctcga tcaggctgga cgacagcacg agcacgatcg
     1681 tgaacgcgca gccgagcgcg ccgatcagga ccgcggacgg gatgcggcgg atcggcacgc
     1741 cgatcgactg cagggccatg ctggccgtgt aggtcgtcat gccgttcagg gcgatcgtgt
     1801 tcacgatgac gccgagcacg agcagcggag cgagccagag cggcagcttc tcgagcagcg
     1861 cgtactcgat gccgttcgcc agggcatccg gcccgaccgt gccgccgagc acgacgccga
     1921 acgccgtgat caggacggcc gggaagacgt tgccgagcgc ggtggctccg atgatgctcg
     1981 acgccttcgt ctcgcggggc aggtagcgtg ccatgtcggc gctgttgctg aacgacagcg
     2041 gcgaggagga cagcagcgcg aaggcgatcg tgatgctcgc ccacagcggc aggccggtca
     2101 gcggttcggg ctgggtgaac ccccagtcga tcgtgggcca gaggaagccg atcacgagcg
     2161 cgaagatcac cgtgaggccg atcgcgatgc ccgagtaggc gcgcaggatc agggcgtgcc
     2221 cgtacacggc gaccagcacg gtgatcgccg cggtgacgac ggccaccacg atcagtgccg
     2281 cggtgtggtc ggggaagccc cagcgcatca gcagctcggc gccgatgaac gacgcggcga
     2341 cccagttcag cgcgaggaac accccggaga tgaaccagcc gtagaacgcg atgacgatct
     2401 tgttgccgtg gatgccgtag atggcgcgct ggatgaccga ccccgacgtg ccggcggccg
     2461 gccccgagat cgccacgatc cccgggaaga ccgcgagcac cgacgaggcc agcagcacca
     2521 gcagcgcctg ccagatctcc agcccgagca tgagcgtgag ggaggcaccg acggtgaggt
     2581 tcagcacgct gatccccggc gccgaccaga cgaagaacag gtctcgcgcc cggccgtggc
     2641 gctcggaatc gtcgatgatc tcgatgccgc gggtctcggg agtgttcgcc gcgtcgacga
     2701 actcggttcc ggcggggtgc gcctcggcgg cgaggcggac gggaacagcg gatgcttcgg
     2761 acattgcggt ccttccagga cggtcgcctc ctgcgtcgct gcaggggcgt tcaggtttca
     2821 ggggttcagg tttcagcggg ttcgggtcac ggtcgccggt aggcggcctc gccgtgcacc
     2881 agggtgagga gcacggcacc ggtgagcagg tcttcggtgg gggcggacag cggatcgatg
     2941 tcgagcacgg cgacgttcgc gtggaagccg ggcgcgatcc gcccgtaggc gtcgtcgatc
     3001 gcgccgacgt acgccgcacc ggaggtggcg gcggcgagag cctgagcggc ggtgaatgcg
     3061 cgctccgggt ggtacggggg cagcgcgctg tcgatggacg atcgccctga catcgcgatg
     3121 aacaggttgt gcagagcctc gtggggtgca gtgggggcgt cggtgcccag cgcgacgggg
     3181 acctcgacat cgcggaacca gttccacggg aagccgtcct cggcgcgctg atcgcccagc
     3241 accgccatcc agttgtccag gatggcgggg tcgcagtgca ccggctgcat cgatgcggcg
     3301 atgccgtgct cccgcatccg ggcgaccgtc tcgggagcga ccgattcgag gtgctcgatg
     3361 cggggccgcc gtccggtgcc gacgccgttc gcctgctcac aggcctcgac gagatccacg
     3421 gcgagcgcac tcgcggcatc gccgatcgcg tgcatcgcga gctgcaggcc ggcgcggtcg
     3481 gccgcgatcg cgaccggcgt cgcggcctcc cggctccaga tcgggtcggc gttcgacccg
     3541 tccgcgtagg ggcgcaccat ggcggcagtg caggcgtcga tcacgccgtc gaggatgaac
     3601 ttcacgccga cgaccttcag ccagggcgtt ccggtcgccg ccagctcgtc gcgcagctgc
     3661 acggcgcgct ccacctgggc gaggtcggcc gccgggtcgc cggtgg
//
LOCUS       DATMSX010000076         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_114730,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000076 DATMSX010000000
VERSION     DATMSX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114730"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VN041_02355"
     CDS             <1..634
                     /locus_tag="VN041_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWU27919.1"
                     /translation="SAQREGAVRAAELERTRIERDLHDGVQPRLVSVGMTLGLAQQKI
                     DTDPAAAKALIDEAHTSTKAAITELRQLARGIHTSVLDDRGLDAALSALASRSHIPVQ
                     LDVRIDAGTTGSGARCRDAEAAIYFAIAESLTNAAKHSRASECRVVVRSRDGGLWARV
                     EDNGMGGAQVQPGGGLDGITNRILAAGGTFRLDSPAGGPTSLEVTVPCAS"
     gene            687..1373
                     /locus_tag="VN041_02360"
     CDS             687..1373
                     /locus_tag="VN041_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU27920.1"
                     /translation="MRILICEDSVLLREGLVRLLEDAGHEVVAALSDTDGLMDAVVDT
                     DPELCILDVRLPPTFTDEGIRAALAIRAGHPSLPLLVLSQYVEERYASDLIAAQGGAL
                     GYLLKDRVADVAEFIESVQRIASGATVLDPEVVAQLLTRRNRDERMLRLTDRERTVLA
                     LIAEGKSNGAIAGLLFLSEASIEKHITAIFQKLGLEPEDGNRRVLAALAHIENTGVAD
                     AGNDYQGGVR"
     gene            1370..2182
                     /locus_tag="VN041_02365"
     CDS             1370..2182
                     /locus_tag="VN041_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HWU27921.1"
                     /translation="MNGATKKTVGALTVVLAVCGGVVLLGTGAGAAFAGVQQVSSQEG
                     GTTRLDVQGVTSLDLEVGAADMTIEFREGVTEARLEIEQGSEQDWSLRRDDDELKVRG
                     PGSGFDWFRPDWLRGEQQATLVLPESLLGIDADLTLQAGSLSVDGEFGELDLDINAGA
                     LTVQGTAREVDAQVNAGRADIDLRDVRTAEYTVNAGRVTAELQSVPDEVSIDVTAGRL
                     DLTVPDGSYALRHQATAGTLESDLVQDPSSTHRIQADVSAGTVTLSPGGAAE"
     gene            2264..2337
                     /locus_tag="VN041_02370"
     tRNA            2264..2337
                     /locus_tag="VN041_02370"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2298..2300,aa:Ile,seq:gat)
     gene            2363..2435
                     /locus_tag="VN041_02375"
     tRNA            2363..2435
                     /locus_tag="VN041_02375"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2396..2398,aa:Ala,seq:tgc)
     gene            complement(2485..3057)
                     /locus_tag="VN041_02380"
     CDS             complement(2485..3057)
                     /locus_tag="VN041_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWU27922.1"
                     /translation="MAGAAHTSTSVGLTLDVISRTVPPAVGLGSAQYADADAHGSTTK
                     VEGVMHVDTSTVALAMTCTVDRENVWAALTERDRLSRWFGELAMAPDDARQADITVSG
                     TGEEVSVRILACEPDCRLSLEWTVDDEVSTVTVVLGDAEGGTAVILQEDGVPEQLTDA
                     YRRGWGAHLRRLDAELSGQSMPEWTELAQE"
     gene            3100..3444
                     /locus_tag="VN041_02385"
     CDS             3100..3444
                     /locus_tag="VN041_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWU27923.1"
                     /translation="MTPAQEELVERIRDLIADEPVQREVSMFGDHCILVNDKMIVGAE
                     KDGGLLVRVSADRNDELILRAGARQAVMGHGREMGPGWIMVDASELDEEQLAFWVDAA
                     LENNAAVTGIGR"
     gene            complement(3451..4002)
                     /locus_tag="VN041_02390"
     CDS             complement(3451..4002)
                     /locus_tag="VN041_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU27924.1"
                     /translation="MTVAQDSAAALRSWTPPRQADRALWQEYVVFADDPAHAHRETST
                     AQHLTASALVFDATLEHVLLCFHGKGRFWVQLGGHIEQSDASLADAALREAREESGLD
                     ALTPLLGTPVDLDRHALSSGFGSCQVHWDIGYAFRAEPDAMPVASDESESVAWWPVRD
                     LPEGSVEGLARRVGRAVEILRAG"
     gene            complement(4021..4218)
                     /locus_tag="VN041_02395"
     CDS             complement(4021..4218)
                     /locus_tag="VN041_02395"
                     /inference="COORDINATES: protein motif:HMM:NF040017.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6186 family protein"
                     /protein_id="HWU27925.1"
                     /translation="MIFSTAFFLLCAAGLILASWRIRLRDTEATVTELFDRLLEDRSV
                     RIAVIVVWWWLGWHFLAGETL"
     gene            complement(4215..>4487)
                     /locus_tag="VN041_02400"
     CDS             complement(4215..>4487)
                     /locus_tag="VN041_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27926.1"
                     /translation="VASWPGAASARRAWTPGLRRLAVAWAVILVAGCLWELAQFVVGR
                     IAPTQPSFALSDLVDPLLDTELGKAVFAGAWLSCGALLVLRGRRSG"
BASE COUNT          730 a         1562 c         1538 g          657 t
ORIGIN      
        1 ctcggcgcag cgcgagggtg ccgtccgggc cgccgagctc gagcgcaccc gcatcgagcg
       61 cgacctgcac gacggcgtcc agccgcgact ggtctccgtg ggcatgacac tgggcctggc
      121 ccagcagaag atcgacaccg accccgccgc tgcgaaggcc ctgatcgacg aggcgcacac
      181 ctccaccaag gccgccatca ccgaactgcg ccagctcgcg cgcggcatcc acacctccgt
      241 gctggacgac cgcggcctgg acgccgcact ctccgcgctg gcgagccgct cgcacatccc
      301 cgtgcagctc gacgtgcgga tcgacgccgg gacgaccggc tccggtgcca gatgccggga
      361 cgccgaggcc gcgatctact tcgccatcgc cgagtcgctg accaacgccg ccaagcactc
      421 cagggccagc gaatgccgcg tggtcgtgcg cagccgagac ggcggcctgt gggcacgcgt
      481 cgaggacaac ggcatgggcg gcgcgcaggt gcagcccggt ggcggactcg acggcatcac
      541 caatcgcatc ctcgccgccg gcgggacctt ccgtctggac agccccgcgg gcggtcccac
      601 cagcctggag gtgacggtgc catgcgcatc ctgatctgcc cgcttctgaa ccagcaccgg
      661 tggtctgaga acgaggaggt ggcgtgatgc ggatcctcat ctgcgaggac tcggtcctcc
      721 tccgcgaagg acttgtccgc ctgctcgagg acgccggcca cgaggtggtc gccgccctct
      781 ccgacaccga cggcctcatg gacgcggtgg tcgacacgga ccccgaactc tgcatcctcg
      841 acgtcaggct cccgccgacg ttcacggacg agggcatccg ggcggccctg gcgatccgcg
      901 ccgggcatcc gtccctgccc ctcctcgtgc tgtcccagta cgtggaggag cgctacgcga
      961 gcgacctcat cgctgcgcag ggcggggcgc tgggatatct gctcaaggac cgggtcgccg
     1021 atgtggccga gttcatcgag tcggtgcagc gcatcgcatc cggcgcgacc gtgctcgacc
     1081 ccgaggtggt cgcgcagctg ctcacgcgcc gcaatcgcga cgagcggatg ctgcgcctga
     1141 cggatcgcga gcgcaccgtg ctcgcgctga tcgccgaggg caagtccaac ggcgccatcg
     1201 ccggcctgct gttcctcagc gaggccagca tcgagaagca catcaccgcc atcttccaga
     1261 agctcgggct cgagccggag gacggcaacc ggcgggtgct ggcggcactc gcgcacatcg
     1321 agaacaccgg cgtcgctgat gccgggaacg actaccaggg aggcgtccga tgaacggcgc
     1381 gacgaagaag accgtgggag cactcaccgt ggtgctggcg gtgtgcggcg gcgtggtgct
     1441 gctgggcacc ggggccgggg cggcgttcgc cggcgtgcag caggtgtcgt cgcaggaggg
     1501 cggcacgacg cggctcgacg tgcagggcgt cacttccctg gatctcgagg tcggcgccgc
     1561 cgacatgacc atcgagttcc gggagggagt gactgaggca cgcctggaga tcgagcaggg
     1621 ctcggagcag gactggtcgc tgcggcgcga tgacgacgag ctcaaggtgc gcgggccggg
     1681 gagcggcttc gactggttcc ggccggactg gctgcgcggc gagcagcagg ccacgctcgt
     1741 gctgcccgag tcgctgctcg gaatcgatgc cgatctcacc cttcaggccg gatcactgag
     1801 cgtggatggc gagttcggcg agctggatct ggacatcaac gccggggcgc tcaccgtgca
     1861 gggtacggcg agggaagtgg atgcccaggt gaatgccggc cgggcggaca tcgacctgcg
     1921 cgacgtgcgc accgcggagt acacggtgaa cgcgggccgc gtcacagcag agctccagtc
     1981 cgttccggac gaggtctcga tcgacgtcac cgccggccgg ctcgacctca ccgtgccgga
     2041 cgggtcgtac gccctgcgtc accaggcgac ggccggaact ctggagagcg acctggtcca
     2101 ggacccctcc agcacgcacc ggatccaggc cgacgtgtcg gccggaaccg tcaccctgag
     2161 ccccggaggt gccgcggagt gaatcggaaa cgcccggcct gcacgcaggc cgggctcgat
     2221 tcgcttttcc tcagcttcat ccggtaaagt cttggaggtt gacggggcta tagctcaggc
     2281 ggttagagcg cttcactgat aatgaagagg tcccaggttc aagtcctggt agccccacac
     2341 attccctcga ggaatcccct tcggggcctt agctcagttg gtagagcgcc tgctttgcaa
     2401 gcaggatgtc aggagttcga atctcctagg ctccacagat caagaccctg gcttccgccg
     2461 gggtctttct ggtttcgcgg ccgctcactc ctgggcgagc tctgtccatt ccggcatcga
     2521 ctggcccgac agctcagcat ccagacgtcg cagatgagca ccccagccgc ggcggtatgc
     2581 gtcggtcagc tgctctggca caccgtcctc ctgcaggatc acagcggttc ccccctcggc
     2641 atcacccagc acgaccgtga ccgtcgagac ctcatcatcg acggtccact ccagggagag
     2701 ccggcagtcg ggttcgcacg cgaggattcg cacggacacc tcttcgccgg tgccggagac
     2761 cgtgatgtcg gcctgccgcg cgtcgtccgg cgccattgcg agttcaccga accagcggct
     2821 gagtcggtcg cgctccgtca aggctgccca gacgttctcg cgatccaccg tgcaggtcat
     2881 cgccagtgcg accgtgctcg tatccacgtg catgactccc tcgaccttgg tcgtcgagcc
     2941 gtgtgcatcg gcatcggcgt actgggccga gcctagtccg accgcagggg gaacggtgcg
     3001 cgagatcacg tccagggtga ggccgacgct ggtgcttgtg tgcgcggcac ctgccacgac
     3061 tgcagggcaa gcagcatctt gttcacgtat tcttcccgca tgactcctgc gcaggaagaa
     3121 ctcgtggagc ggatccgcga cttgatcgcc gacgagccgg ttcagcgcga ggtgtcgatg
     3181 ttcggcgatc actgcatcct ggtgaacgac aagatgatcg tcggcgcaga gaaggacggc
     3241 ggtctgctgg tgcgggtctc cgccgaccgg aacgacgagc tcattctgcg tgcgggcgcg
     3301 aggcaggcgg tcatgggtca cggccgggag atggggccgg gctggatcat ggtggatgcc
     3361 agtgaactgg atgaggagca gctggccttc tgggtcgatg ccgccctgga gaacaacgcg
     3421 gcggtcaccg gcatcggccg ctgaccggcc tcagcctgcg cgcaggatct cgacggcccg
     3481 gccgacgcgc cgcgccagtc cctcgacgct gccctcgggc aggtcacgca caggccacca
     3541 cgcgacgctc tcgctctcgt cgctggcgac gggcatcgca tccggttcag cgcggaacgc
     3601 gtagccgatg tcccagtgca cctggcaact cccgaatccc gacgagaggg cgtgccggtc
     3661 gaggtcgacg ggtgtgccga gcaggggcgt gagggcgtcc agcccgctct cctcgcgcgc
     3721 ctcgcgcagc gcggcatccg ccagggacgc atcagactgc tcgatgtgcc cgcccaactg
     3781 cacccagaag cgccccttcc cgtggaagca gagcagcacg tgctccagcg tcgcgtcgaa
     3841 gaccagcgcg ctcgccgtca ggtgctgggc cgtcgacgtc tcgcggtgtg cgtgtgcggg
     3901 atcgtcggcg aagaccacgt actcctgcca cagggcccga tcggcctgcc ggggaggagt
     3961 ccacgagcgg agggcggcgg cggaatcctg ggcgacggtc accacctcag cgtaggcggg
     4021 tcacagggtc tcgcccgcca ggaagtgcca gcccagccac caccacacca cgatcacggc
     4081 gatccgcacg gaccggtcct cgagcagacg gtcgaacagc tccgtgacgg tcgcctccgt
     4141 atcccgtagc cggatgcgcc aggatgccag gatcagccct gccgcgcaga gcaggaagaa
     4201 cgcggtgctg aagatcatcc gctgcgccgt cccctcagca ccaggagcgc gccgcagctg
     4261 agccaggcac cggcgaagac cgccttgccg agctccgtgt ccagcagcgg atcgacgagg
     4321 tcgctcagcg cgaagctcgg ctgcgtcggg gcgatccggc cgacgacgaa ctgcgccagc
     4381 tcccacaggc atccggccac caggatcacc gcccacgcca cggcgagtcg tcgcagcccg
     4441 ggcgtccagg cccggcgagc ggatgccgca cccggccagc tcgccac
//
LOCUS       DATMSX010000077         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_105288,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000077 DATMSX010000000
VERSION     DATMSX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105288"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1418)
                     /gene="nadA"
                     /locus_tag="VN041_02405"
     CDS             complement(120..1418)
                     /gene="nadA"
                     /locus_tag="VN041_02405"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWU27927.1"
                     /translation="MSETSLLDPSVDHAIQAIVLGGSTESTCTTDLAAGPWDFDIRPG
                     YGPGSSMGDVIPTGSPRQGELPQEYRDAGEDELHDRIRAAKLTLGERVVVLGHFYQRE
                     EVVRHADYVGDSFQLANAALEHPDAEAIVFCGVHFMAETADLLSGPEQSVILPNLAAG
                     CSMADMADIDQVEDCWEQLADVYGPLDAADEEGLVPVIPVTYMNSSAAIKGFVGRHGG
                     IVCTSSNAATVLEWAFARGRRVLFFPDQHLGRNTAKAMGVELERMPMWNPRRPLGGST
                     AGELRDARVILWHGFCSVHRRFSVDQIDKARAEHPGVRVIVHPECPMEVVDAADEAGS
                     TDYIRKAIAAATEPTAFAIGTEINLVRRLAAQFPQHEIFCLDPVVCPCSTMYRIHPGY
                     LAWVLEELVARRVVNRIRVSDDVASPARVALERMLAAKPR"
     gene            complement(1526..2191)
                     /locus_tag="VN041_02410"
     CDS             complement(1526..2191)
                     /locus_tag="VN041_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU27928.1"
                     /translation="MSGTTGEHDAIRVAVSTVIFSLRSAPDGDRLMLPLVRRTREPHL
                     GAWALPGGWLDPAEDLDAAASRTLAETTALKPSYLEQLYTFGDVDRAPSRAISIVYWA
                     LLRSDLVDAQRSAADAPENVEWFDVDAVPPLAFDHNRIAEYALDRLRNKVGYSRIAAG
                     LLPDEFTLTELREVYEAVLGRRLDPSNFRRLLEGSDELVPTESFRTGKHRPARLYRYN
                     ADS"
     gene            2222..3133
                     /locus_tag="VN041_02415"
     CDS             2222..3133
                     /locus_tag="VN041_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU27929.1"
                     /translation="MPASDTTPGPVTDAEVPAYLSALRIPGLADVHVHFLPERMLDKV
                     WSYFDAAEQNYGYAWPIQYRYDDETRLAVARELGMRAIPALTYPHKPGMAAWLNDWNR
                     RFAAAHGDVVHCATLFAEPDSASYVPEALEAGARLFKVHVQVGGFAPDDPVLEAAWTA
                     LERAGVPVVIHAGSRPLPGAHTGPDAIGAVLARHPRLAFVIAHMGMPEYDAFADLAEQ
                     YENVHLDTTMFATGFLDGPSVWPDGYLDRLGALQERIVLGSDFPNIPYPYARQLSALA
                     DLGLGDDWMRSVLWRNGARLLGLRDAG"
     regulatory      3213..3306
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3474..>4341
                     /gene="gcvT"
                     /locus_tag="VN041_02420"
     CDS             3474..>4341
                     /gene="gcvT"
                     /locus_tag="VN041_02420"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWU27930.1"
                     /translation="MTEPRYTPLRARHEALGASFTDFGGWQMPVRYTSDLAEHHAVRE
                     AAGIFDISHMAEFTVRGADAAAYLDYSLAGRLSAMTVGKAKYSLLLAEDGGIIDDVIV
                     YRLGDDDFLIISNAGNRDAVREALLARTDGFDVETVDVSDDYALIAVQGPAAEQILAE
                     VAGIGDVSIPWAEQKYYAWADATWNGKPLLLARTGYTGEDGFELLVAAADAESLWDAV
                     LAVGGLKGLVPCGLASRDTLRLEAGMPLYGHELNREIKPAQAGLGRVVVTNKERFVGQ
                     GAVTPAEGAPVLV"
BASE COUNT          651 a         1511 c         1539 g          640 t
ORIGIN      
        1 gtgcgcggtg cagccctcac ccagcacgcc cttcaccagc acctcgacaa cgtggccgag
       61 ggcatcggcg cggagcgcgg cggtcagtcc ggcgatgccg gcgcccgcga tcaggatctt
      121 catcgcggct tcgccgccag catccgctcc agcgccacgc gggccgggct cgcgacatcg
      181 tccgagacgc ggatgcggtt gaccacgcgc ctggcgacca gttcctccag cacccaggcc
      241 agatagccgg ggtggatgcg gtacatggtc gagcaggggc agacgacggg atccaggcag
      301 aagatctcgt gctgcgggaa ctgcgcggcg agccgccgga cgaggttgat ctcggtgccg
      361 atcgcgaagg cggtgggctc cgtggccgcg gcgatcgcct tgcggatgta gtcggtggag
      421 ccggcttcgt ccgcggcgtc gacgacctcc atcgggcatt ccgggtggac gatcacgcgc
      481 acgcccgggt gctcggcgcg ggccttgtcg atctggtcga cgctgaaccg gcggtgcacc
      541 gagcagaagc cgtgccacag gatgacacgg gcatcgcgaa gctcgccggc ggtggaaccg
      601 ccgagcggac gccgcgggtt ccacatcggc atccgctcca gctccacgcc catcgccttg
      661 gcggtgttgc gccccagatg ctggtcgggg aagaacagca cgcggcgacc ccgggcgaac
      721 gcccactcca gcacggtcgc ggcgttcgag gaggtgcaga cgatgccgcc gtgccgcccg
      781 acgaatccct tgatggctgc cgaggagttc atgtaggtga ccgggatcac gggcacgagg
      841 ccctcctcgt cggccgcgtc cagcgggccg tatacatcgg cgagctgctc ccagcagtcc
      901 tccacctggt cgatgtcggc catgtcggcc atcgagcagc ccgcggcgag gttcggcagg
      961 atcacggact gctcggggcc ggagagcagg tcggcggtct cggccatgaa gtgcacgccg
     1021 cagaacacga tcgcctcggc atccggatgc tccagcgcgg cgttcgcgag ctggaaggag
     1081 tcgcccacgt agtcggcgtg ccgcacgacc tcctcgcgct ggtagaagtg gccgagcacc
     1141 acgacgcgct cgccgagggt cagcttggcc gcacggatgc ggtcgtgcag ctcgtcctcg
     1201 ccggcatcgc ggtactcctg cggcagctcg ccctggcgcg gcgaccccgt ggggatgacg
     1261 tcgcccatcg aggagccggg accgtagccg gggcggatgt cgaagtccca gggacccgcc
     1321 gcgaggtcgg tggtgcacgt cgactccgtg gagccgccca gcacgatcgc ctggatcgcg
     1381 tggtcgaccg aggggtcgag cagagaggtt tcagacatcg ggtgccgcct tctggtcact
     1441 tcgacactta gtgtgcggga tcagattatg tcgatctgac cagaagtgca atcggaggga
     1501 cgcgacgatg ccgcgcgcct cccgctcagg agtccgcgtt gtagcggtac agacgcgccg
     1561 gacgatgctt gccggtgcgg aacgactcgg tcggcaccag ttcgtccgat ccctccagca
     1621 ggcggcggaa gttggagggg tccaggcgac ggcccagcac cgcctcatag acctcgcgca
     1681 gctcggtgag ggtgaactcg tccggcagca ggcccgcggc gatgcggctg tagccgacct
     1741 tgttgcggag gcggtccagg gcgtactcgg cgatgcggtt gtggtcgaag gccagtggcg
     1801 ggacggcgtc gacatcgaac cactcgacgt tctccggggc atccgccgct gagcgctgcg
     1861 catcgacgag gtccgatcgc agcagtgccc agtacacgat cgagatggcg cggctgggcg
     1921 cgcggtcaac gtcgccgaac gtgtacagct gctcgaggta gctcggcttc agcgccgtgg
     1981 tctcggcgag cgtgcgagat gccgcggcat ccaggtcctc ggccggatcc agccatccgc
     2041 cgggcagcgc ccatgctccc aggtgcggtt cgcgcgtgcg gcgcaccagc ggcagcatga
     2101 gccgatcgcc gtccggcgcc gagcgcagcg agaagatgac ggtcgacacg gcgacgcgga
     2161 tcgcgtcgtg ctcgcctgtc gtgccactca caccgacgag cctaggggag aggatggggg
     2221 catgccggca tccgacacca ctcccgggcc cgtgaccgac gccgaggtcc ccgcctacct
     2281 ctccgcgctg cgcatccccg ggctcgccga cgtgcacgtg cacttcctcc cggaacggat
     2341 gctggacaag gtctggtcct atttcgacgc agccgagcag aactacggct acgcctggcc
     2401 catccagtac cggtacgacg acgagacccg gctcgccgtc gcgcgcgagc tggggatgcg
     2461 ggcgatcccg gcgctgacct atccgcacaa gccggggatg gccgcctggc tgaacgactg
     2521 gaatcggcgt ttcgcggcgg cgcacggcga cgtcgtgcac tgcgcgacgc tgttcgcgga
     2581 accggactcc gcgtcctacg tgcccgaggc gctcgaggcc ggggcgcggc tgttcaaggt
     2641 gcacgtccag gtcggcgggt tcgcgccaga cgatccggtg ctggaggcgg cgtggacggc
     2701 gctggagcgt gccggcgtgc ccgtggtcat ccatgccggc tcgcggccgc tgcctggtgc
     2761 gcacaccggg ccggatgcca tcggcgcggt gctcgcgcgc cacccgcgcc tcgctttcgt
     2821 catcgcgcac atgggtatgc cggagtacga cgccttcgcc gacctggccg agcagtacga
     2881 gaacgtgcac ctggacacga cgatgttcgc gaccgggttc ctggacgggc cgtccgtgtg
     2941 gccggacggg tacctcgacc gcctcggcgc gctgcaggag cgcatcgtgc tcggatcgga
     3001 cttcccgaac atcccgtacc cgtacgcgcg gcagctgtcc gccctcgccg acctcgggct
     3061 cggagacgac tggatgcgct cggtcctgtg gcgcaacggc gctcgtctcc tggggctccg
     3121 cgacgccggc tgacgggatc ggctccgccg cggaacgccg cgggacacgc cgccgtgccg
     3181 ctatgctgga ggcctaactg aacacaggcc gaacgacctg tgcgggagag tccggtgatc
     3241 gcagccggac accgaaggag caagcctccc cgccaatctc tcaggtaccc ctaccgtgct
     3301 ggtccggccg ctctgaaaag cgatgctccg gcatccgccg acggtgaaag cctctctcct
     3361 gacatgggtc cccgagaccg tcgcagggtg ggtgaagctc tcaggcccga tgacagaggg
     3421 ggagttccga ggacgccgat gatgtcgtcc tgcaacccga ccgggagaac tccgtgaccg
     3481 aaccccgtta caccccgctc cgtgcgcgcc atgaggcgct cggtgcctcc ttcaccgatt
     3541 tcggcggctg gcagatgccg gtgcgctaca cctccgacct cgccgagcac cacgccgtgc
     3601 gcgaggccgc cgggatcttc gacatctcgc acatggccga gttcaccgtg cggggtgctg
     3661 acgccgccgc gtacctggac tactcgctcg cgggccgcct gtcggcgatg accgtgggca
     3721 aggcgaagta ctcgctgctg ctcgccgagg acggcggcat catcgacgac gtgatcgtct
     3781 accgcctcgg cgacgacgac ttcctgatca tctccaacgc ggggaaccgc gacgcggtgc
     3841 gcgaggcgct gctcgcccgc accgacgggt tcgacgtcga gacggtcgac gtctccgacg
     3901 actacgcgct gatcgccgtg cagggccccg ccgccgagca gatcctcgcc gaggtcgccg
     3961 gcatcggcga cgtctcgatc ccctgggccg agcagaagta ctacgcctgg gccgacgcga
     4021 cgtggaacgg caagccgctg ctgctcgccc gcaccgggta caccggcgag gacggcttcg
     4081 agctgcttgt ggccgccgcc gacgcggagt cgctctggga cgccgtgctg gccgtcggcg
     4141 ggttgaaggg cctcgtgccc tgcggcctcg cctcccgcga cacgctgcgc ctcgaggcgg
     4201 gcatgccgct gtacggccat gagctgaacc gcgagatcaa gcccgcgcag gccgggctcg
     4261 gccgcgtggt cgtgaccaac aaggagcgct tcgtcgggca gggcgccgtg acgcctgccg
     4321 agggggcccc cgtgctcgtc g
//
LOCUS       DATMSX010000078         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_67386,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000078 DATMSX010000000
VERSION     DATMSX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67386"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..938
                     /locus_tag="VN041_02425"
     CDS             336..938
                     /locus_tag="VN041_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWU27931.1"
                     /translation="MARDFMTDPGQLRDLEGQQYVVLRPTGAVMHEWDRSRDTAQRRL
                     GGTVRYPGAAHVTLRGLYEPERVDEVRAVIGEWAAQQHPIELVTEAVDSFPAPWQIVI
                     ARLARTPSLVHAYASLTDALDRTDLRRIGELPLDDWTFHLSTVYAKALDAEDWARLAS
                     DTRHDYPTPPVETVAEVELVTYRDATEHREVIALGGRGVS"
     gene            complement(931..1893)
                     /gene="ccsB"
                     /locus_tag="VN041_02430"
     CDS             complement(931..1893)
                     /gene="ccsB"
                     /locus_tag="VN041_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWU27932.1"
                     /translation="MPDLDSISRLTLWTAIAIYALAFIAYAFDLARRSSTVKAEERML
                     VMSDGTVSQAPVDGDVDAPVGKRFVMARIGTSLMVLGWLFHLAATLTRGFANGHVPWA
                     NLYEFAMTGTLLVVLVYLVMLTRIDLRYLGTFISGIVLVLLGAASAYFYVEVKPLADP
                     LKSVWLVIHVFVASLSVGIFALAFALSVMQLLQSRRERRVAEGIANAGPGFLRTLPDT
                     VRLENLAYRFAVVGFIFWTFTLIAGSIWAYFAWSRFWGFDVKETWTFIIWVLYAGYIH
                     ARATRGWRGTPSAWLSITGFAAVIFNFTIVNVYFKGLHAYSGLS"
     gene            complement(1895..3583)
                     /locus_tag="VN041_02435"
     CDS             complement(1895..3583)
                     /locus_tag="VN041_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWU27933.1"
                     /translation="MSDPLRPADHIDSADAADDGITQPKLGFVGWLRWGWRQLTSMRV
                     ALILLLFLAIGAVPGSVFPQRSADPNGVLKYKTDNPDLFPILDKLSMFDVYSSPWFSA
                     VYILLFISLIGCIIPRIKHHAKALRALPPRTPARLARLEDHRSVTRALTGTATQDADA
                     AASIDVAEKQLKALGYRVARYDRGRVWSVSAERGYWRETGNLIFHIALVGVLITVGVG
                     GGFAYTGQRVVVEGKSYVNALIDYNSINRGRFVPDDAFVPYALTLDSFDVTYEAVGAP
                     GAGQAGNFKANLSVTEPDGAKRKAAVEVNHPLSVGGDNIYLLGNGYAPSITVRNAQGV
                     EVFSGPVEFLPQDNNMTSLGVLKIPDGMPKQLGLVGFFYPTAVKLPSGAFTSLRGDLN
                     IPLLTLDVYQGDLGIDGGNPVSVYQLDTNDMTKLNGRGTDAKSIQLQPGETADLPDGL
                     GTVTFENEAPAGAKDFSKSVKRYVSLQIHHDDSVVWVLVFALLALAGLGLALFVPRRR
                     MWVKATASDDSIHLEYAGLARGEDPTLAAAVDDLVRGHERLLDAAASRHPVKGA"
     gene            complement(3633..4406)
                     /locus_tag="VN041_02440"
     CDS             complement(3633..4406)
                     /locus_tag="VN041_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HWU27934.1"
                     /translation="MNPDAVIGAGALWLAIPLALLAGLVSFLSPCILPLVPGYLGFIG
                     GAAGASTAQGGASTGSASRASGRRRLLLGVLLFILGFTIVFVAITALSGTFGAFVLQW
                     SDLITRIFGALIIVMGFVFLGFFGFAQREMRLHVQSTTGLVGAPLLGIALGLSWAPCT
                     GPTLGAIVALSFNAGDPWRAALLGVAYSLGLGIPFLLVALGFGWATSATAFMRRHIRT
                     VNIIGGALLIVLGLLMVTGLWTQIMSNIGAVMSSVILPL"
     gene            complement(4403..5023)
                     /locus_tag="VN041_02445"
     CDS             complement(4403..5023)
                     /locus_tag="VN041_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWU27935.1"
                     /translation="MSSPARSRRDVLRIGAGALAGALAVGLSACAPDPATDAFLNGKE
                     KAYTADDLRVVEIPADKRGEPVVFGGTTESGEKFSSADSLGEVIVVNFWYATCGPCRV
                     EAKDLETVWQKHKGDGVVFMGVNIYDQAATAKAFAEKYGVTYPSLLDADSGQAKLAFA
                     KVAPIQAPPTTLVLDRKGRVAARIIGPIDGTSILSTLIADALEEKA"
     gene            complement(5069..5509)
                     /locus_tag="VN041_02450"
     CDS             complement(5069..5509)
                     /locus_tag="VN041_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU27936.1"
                     /translation="MKSSPDRPWSVGEVAERFDLPTNVLRHWESVGLLSPARDGADRR
                     RYGPDDVVRVAVVLRSKAAGMSLDQIAHLLERDMSGRHQVLQAHLDDLDRRMAEMRHS
                     KAMTEHALQCSAHDISTCPRFRAELADVLVGFDPAIDAKVPAGV"
     gene            5567..>6398
                     /locus_tag="VN041_02455"
     CDS             5567..>6398
                     /locus_tag="VN041_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU27937.1"
                     /translation="MRDVIIIGAGPAGLQAALTLGRMHRTALLVDSGAYRNGTVRHMH
                     NVIANDGTPPADFRRVAREQLDGYATVETLEGTIDRVDAVDGGFLVTFSDGRAESASR
                     LLLATGMADELPDIEGLAPLWGLRAFSCPFCDGHEFAGRRIGLVGESSRFEHLIRLLR
                     PIVADIVVFDGDALEDEQRLSLQEAGAVIHPGRASAVAEHGDGVRVTAGDTVDVAGLF
                     VATGPARQRAPFAAQLGLTVLPSGAIEIDDFGRTSRPGVWAAGDIAHRASLPGAMASV
                     L"
BASE COUNT         1063 a         2154 c         2266 g          915 t
ORIGIN      
        1 ctcggggccg cgatgccgac ggcgcccgca ctcggcggcc tgatcattcc caacggcgcc
       61 gcgttcgcgg gtctcgtggt gtgggcggtg ctgtcactgg gggcagcgac actcgccgtg
      121 gcgacgcgtc gcacgacctc cgcgaagcag gtgctcgcca cggcctagca tccgctcctc
      181 cgctcccccc gcttccggag ctcctggagc tccgggagca ggaggattgc cgggggcagg
      241 acgatgtcac gggagatcgt cctgcccccg gcattcgtcc tgttctcgag gacatgagac
      301 gacgtgcctg aacgactgcc gggtagcgtc gtcgcatggc gcgcgacttc atgaccgacc
      361 ccgggcagct gcgagacctg gagggacagc agtacgtcgt gctgcggccg accggcgccg
      421 tcatgcacga gtgggatcgg agccgcgata ccgcgcagag gcgcctgggc gggaccgtgc
      481 gctacccggg tgccgcgcac gtcacgctgc gggggctcta cgagccggaa cgcgtcgacg
      541 aggtgcgggc cgtcatcggc gagtgggccg cacagcagca tccgatcgag ctcgtcaccg
      601 aagccgtcga ctccttcccc gcgccgtggc agatcgtgat cgcgcggctc gcgcgcactc
      661 cgtcactcgt gcacgcctat gcgtcgctga ccgacgcgct cgaccgcacc gatctgcgcc
      721 gcatcggcga gctgccgctg gacgactgga cgttccacct gtcgacggtc tacgccaagg
      781 cgctggatgc cgaggactgg gcgcggctcg catccgacac ccgtcacgac tacccgacgc
      841 cgccggtgga gaccgtcgcc gaggtcgagc tcgtgaccta tcgggacgcg accgagcacc
      901 gcgaggtgat cgctctcggc ggtcgaggag tcagctgagg ccggagtacg cgtgcaggcc
      961 cttgaagtac acgttcacga tcgtgaagtt gaagatcacg gcggcgaagc cggtgatcga
     1021 cagccaggcc gacggggtgc cgcgccagcc gcgcgtcgcg cgggcgtgga tgtatccggc
     1081 gtagagcacc cagatgatga acgtccaggt ctccttcacg tcgaagcccc agaacctgct
     1141 ccaggcgaag tacgcccaga tcgaccccgc gatcagcgtg aaggtccaga agatgaagcc
     1201 cacgacggcg aagcggtacg ccaggttctc caggcgcacc gtgtcgggca gggtgcgcag
     1261 gaagccgggg cccgcgttcg cgatgccctc cgcgacgcgg cgctcacgcc gggactgcag
     1321 cagctgcatg accgagagcg cgaaggccag agcgaagatt ccgaccgaca gcgacgccac
     1381 gaagacgtgg atgacgagcc acaccgactt cagcgggtcg gccagcggct tcacctcgac
     1441 gtagaagtag gcggatgccg cgcccagcag caccagcacg atgcccgaga tgaacgtgcc
     1501 gaggtagcgc aggtcgatgc gggtgagcat caccaggtac acgagcacga ccagcagcgt
     1561 gccggtcatc gcgaactcgt acaggttcgc ccacggcacg tggccgttgg cgaagccgcg
     1621 ggtgagggtc gccgcgaggt ggaacagcca gcccagcacc atcagcgagg tgccgatgcg
     1681 cgccatcacg aagcgcttcc cgaccggtgc gtccacgtcg ccgtcgaccg gcgcctgcga
     1741 caccgtgccg tcggacatga ccagcatccg ctcctcggcc ttcaccgtgg aggagcgccg
     1801 tgccaggtcg aacgcatagg cgatgaaggc cagtgcgtag atggcgatgg ccgtccacag
     1861 cgtcagacgc gagatggaat ccagatcggg catgtcatgc tcccttcacg gggtgccgcg
     1921 aggcggccgc atcgagcagc cgctcgtgcc cgcggacgag atcgtccacg gctgcggcca
     1981 gggtcgggtc ctcgccgcgc gcgaggccag cgtactccag gtggatggaa tcgtcggatg
     2041 ctgtcgcctt gacccacatg cgacggcgcg ggacgaacag cgcgaggccg agcccggcca
     2101 gggcgagcag ggcgaacacg agcacccaga cgaccgagtc gtcgtggtgg atctgcagcg
     2161 agacgtagcg cttgacggac ttgctgaagt ccttggcgcc tgcgggcgcc tcgttctcga
     2221 aggtgaccgt gccgagcccg tcgggcaggt ccgcggtctc acccggctgc agctggatgg
     2281 acttcgcgtc cgtgccgcgg ccgttgagct tggtcatgtc gttggtgtcg agctggtaga
     2341 ccgagaccgg gttgccgccg tcgatgccca ggtcaccctg gtagacgtcg agcgtgagca
     2401 gcgggatgtt cagatcgcct cgcagggagg tgaacgcacc gctggggagc ttgactgcgg
     2461 tcgggtagaa gaacccgacc agcccgagct gcttcggcat cccatctggg atcttcagca
     2521 cgcccagcga ggtcatgttg ttgtcctgcg gcaggaactc gacaggtccg ctgaagacct
     2581 cgacaccctg ggcgttgcgc accgtgatgc tgggcgcgta gccgttgccg agcaggtaga
     2641 tgttgtcgcc gccgacgctc agcgggtggt tgacctcgac ggccgccttg cgcttcgccc
     2701 cgtcgggctc ggtcaccgag aggttggcct tgaagttgcc ggcctgaccg gcaccggggg
     2761 ccccgacagc ctcgtacgtg acatcgaagg agtccagcgt gagggcgtag ggcacgaaag
     2821 cgtcgtcagg cacgaagcgc ccgcggttga tcgagttgta gtcgatcagg gcgttcacgt
     2881 aggacttgcc ctccaccacc acgcgctgac cggtgtaggc gaacccgccg cccacaccga
     2941 cggtgatgag gacgccgacg agggcgatgt ggaagatgag gttgccggtc tcgcgccagt
     3001 agccgcgctc ggccgacacc gaccagaccc gcccgcggtc gtaccgcgcg acgcggtagc
     3061 cgagcgcctt gagctgcttc tcggccacgt cgatggacgc cgcggcatcg gcatcctggg
     3121 tcgcggtgcc cgtcagggca cgcgtgacgg accggtggtc ctccagccgc gcgagccgag
     3181 ccggcgtgcg cggcggaagc gcgcgcagcg ccttcgcatg gtgcttgatc cgcgggatga
     3241 tgcagccgat cagcgagatg aacagcagga tgtagacggc cgagaaccac ggcgacgagt
     3301 agacgtcgaa catgctgagc ttgtccagga tcgggaacag atccgggttg tcggtcttgt
     3361 acttcagcac gccgttcggg tcagcggacc gctgcgggaa caccgagccg gggacggcgc
     3421 cgatggcgag gaacagcagc aggatcagcg cgacgcgcat cgaggtcagc tgacgccagc
     3481 cccatcgcag ccacccgacg aatccgagct tcggctgcgt gatgccgtcg tcggcggcat
     3541 ccgccgagtc gatgtggtca gcagggcgca gcggatcgga caccgaggac gagccgtcct
     3601 ggccgccctt cgccttgcga ttgcgagtgc cctcagaggg ggaggatgac actgctcatc
     3661 accgccccga tgttcgacat gatctgggtc cacaggccgg tgaccatgag caggcccagc
     3721 acgatcagga gcgcgccgcc gatgatgttg acggtgcgga tgtggcggcg catgaacgcg
     3781 gtcgccgagg tcgcccagcc gaagcccagg gcgaccagca ggaacgggat gcccaggccc
     3841 agcgagtacg cgacgccgag cagggccgcg cgccacgggt cgccggcgtt gaacgacagc
     3901 gccacgatcg caccgagtgt cggccccgtg cacggagccc agctcaggcc cagtgcgata
     3961 cccagcagcg gcgcccccac gagccccgtg gtggactgca cgtgcagtcg catctcgcgc
     4021 tgcgcgaagc cgaagaagcc caggaaaacg aaccccatga cgatgatcag ggcgccgaag
     4081 atccgcgtga tcaggtccga ccactgcagc acgaacgcgc cgaaggtccc gctgagagcc
     4141 gtgatcgcga cgaacacgat cgtgaagccg aggatgaaca gcagcacgcc cagcaggagc
     4201 cggcgtcggc cggatgcccg ggatgccgag cccgtcgacg cgccgccctg agccgtcgac
     4261 gccccggccg ctcccccgat gaacccgagg taaccgggca cgagcggcag gatgcacggg
     4321 gagaggaacg acacgaggcc ggccagcagc gccaggggga tcgccagcca gagggcgccg
     4381 gcgccgatca cggcgtccgg attcacgcct tctcctcgag ggcgtcggcg atcagggtcg
     4441 acaggatcga cgtgccgtcg atggggccga tgatgcgggc tgccacgcga cccttgcggt
     4501 cgagcacgag cgtggtcggg ggcgcctgga tgggtgcgac cttggcgaac gcgagcttgg
     4561 cctgaccgct gtcggcgtcc agcaggctcg ggtaggtgac gccgtacttc tcggcgaagg
     4621 ccttggcggt ggcggcctgg tcgtagatgt tcacgcccat gaacacgacg ccgtcgccct
     4681 tgtgcttctg ccacacggtt tcgaggtcct tggcctccac gcggcacggg ccgcaggtgg
     4741 cgtaccagaa gttcacgacg atcacctcgc cgaggctgtc ggcgctggag aacttctcgc
     4801 ccgactcggt ggtgccgccg aagacgacgg gctcaccgcg cttgtcggcg gggatctcga
     4861 cgacgcggag gtcgtcggcc gtgtaggcct tctccttgcc gttcaggaac gcgtcggtcg
     4921 cggggtcggg agcgcaggcg ctcaggccca cggcgagcgc gccggcgagg gccccagcgc
     4981 cgatgcgcag aacatcgcgg cgggatcgag caggcgagga cacgctggga atcgtacgcg
     5041 cgtttcctat gggtgctctg gaacggtgct atacgcccgc gggcaccttc gcgtcgattg
     5101 cgggatcgaa cccgaccagc acatcggcga gttccgcacg gaagcgcgga cacgtcgaga
     5161 tgtcgtgcgc cgagcattgc agcgcgtgct cggtcatcgc cttcgagtgc cgcatctcgg
     5221 ccatccggcg gtcgagatcg tccagatggg cctgcagcac ctggtgccgt ccggacatgt
     5281 cgcgttcgag cagatgcgcg atctggtcca ggctcatgcc ggccgccttg ctgcgcagca
     5341 cgacagccac acgcaccacg tcgtcgggcc cataacggcg ccgatccgcg ccgtccctgg
     5401 ccggcgagag cagaccgacc gattcccagt gccgcagcac attcgtgggc aggtcgaacc
     5461 gctcggcgac ctcgccgacg gaccacgggc gatcgggact tgacttcatg ttgacatgaa
     5521 gtcacagact ggcggggaaa gcaacccgac ccccaggagg atcaccatgc gcgacgtcat
     5581 catcatcggt gcgggccccg ccggcctgca ggccgccctg accctcggac gcatgcaccg
     5641 caccgcactg ctcgtcgatt cgggcgcgta ccgcaacggc accgtgcgtc acatgcacaa
     5701 cgtcatcgcc aatgacggca cgccgccggc agacttccgc cgagtggcac gggagcagct
     5761 cgacgggtac gccaccgtcg agacgctcga gggcaccatc gatcgggtgg acgcggtcga
     5821 cggcgggttc ctcgtgacct tctcggacgg acgggccgag agcgcctccc ggctgctgct
     5881 cgccaccggc atggccgacg agctgccgga catcgagggc ctggctcccc tgtggggact
     5941 gcgcgccttc agctgcccgt tctgcgacgg tcacgagttc gcgggccgca ggatcgggct
     6001 cgtcggtgag agctcccgct tcgagcacct gatccgcctg ctgcgtccga tcgtcgcgga
     6061 catcgtggtg ttcgacggcg atgcgctgga ggacgagcag cgcctgtccc tccaggaagc
     6121 cggggctgtc atccacccgg ggcgcgcctc agcggtcgcc gagcacggcg acggcgtgcg
     6181 ggtgaccgcg ggcgacacgg tcgacgtggc ggggctcttc gtggccacgg ggcctgcgcg
     6241 tcagcgcgcg ccgttcgccg cgcagctggg tctcacggtg ctgcccagcg gagcgatcga
     6301 gatcgatgac ttcggcagga cgtcccgtcc cggagtctgg gcggccggtg acatcgcgca
     6361 ccgggcgagc ctccccggcg ccatggcgtc cgtgctcg
//
LOCUS       DATMSX010000079         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_181116,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000079 DATMSX010000000
VERSION     DATMSX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181116"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN041_02460"
     CDS             complement(<1..282)
                     /locus_tag="VN041_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HWU27938.1"
                     /translation="MASSEAEREAMTRALALALNGPRGLNPQVGAVILSAAGEVIAEG
                     WHRGSGTPHAEVDALSKLSRGQARGATAVVTLEPCNHTGRTGPCSQALID"
     gene            complement(714..1577)
                     /locus_tag="VN041_02465"
     CDS             complement(714..1577)
                     /locus_tag="VN041_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWU27939.1"
                     /translation="MPEMPEVQGLVDFLGERAVGHAITRTSVGEIAALKTYDPPNTAL
                     HGATITATARYGKFVDIACGDDLHLVFHLAKAGWLRWYDALPTTLIKPGRSPIALRIA
                     LDDGSGFDLTEAGTKKSLAVYVVRDPQEVPGIARLGPDPLDPSFTRDVFASLLEGRRM
                     QIKGLLRDQGVIAGIGNAYSDEILHVAKMSPYAPAGNLDDDAIDRLYTAMQDTLREAV
                     RVASGKPPAELKDAKRAGFRVHARRGEACPVCGDTVRSVFFADRSLEYCPTCQTGGKP
                     LADRRLSRLLK"
     gene            complement(1618..2181)
                     /locus_tag="VN041_02470"
     CDS             complement(1618..2181)
                     /locus_tag="VN041_02470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWU27940.1"
                     /translation="MEPVTLRTERLVLSIPTEADVDAITAACQDPEVPRWTTVPSPYS
                     RQDAEDFVALVASWWADGVETVWAIRRDGAFAGMIGLHKITQRADGGEAEIGFWGVAE
                     QRGQGVMTEAARAVVDFGFGELGLARVEWRAVVGNIPSARTARALGFRYEGLLRQGHS
                     SPRGRDDAWIAGLLASDDRTPVEWPVL"
     gene            2253..3158
                     /locus_tag="VN041_02475"
     CDS             2253..3158
                     /locus_tag="VN041_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWU27941.1"
                     /translation="MPMQRSSPKGVLVSLLASVLFASIFYLTVQLSSSAEVVWAWRVF
                     VTLALYLLALWHPGAREALRVLIRRLRARWWMSLLALLLASIVGFQLWLFMWAPMHGH
                     GLDASLGYLLLPITLVLGGRFILHAPVSRVQWCVVALALLAVAVKFAATPQLSWVTIV
                     ICIPYAVYFVLRQRFGLDGPLVFGMELAVMSPVAAVVFLTATPGPLPIAELGGLALVG
                     LLGGIGMVLYLGASTMLTMPVFGLLTYVEPVLLVGVAFVLGERMQGADAVVYLILAVA
                     LALLGVEGFRAARRRTTDPDVGAAE"
     gene            3168..>3547
                     /locus_tag="VN041_02480"
     CDS             3168..>3547
                     /locus_tag="VN041_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWU27942.1"
                     /translation="MTVPGILRALEEANLYRDALTWAQTDGEISVVDGLDAPVIAGLL
                     RKRAQAGHPASALVVTPTGRRAESVAAALSTYLADAEILTFPAWETLPHERLSPSPDT
                     VGRRLETLRRIRTWSGEKPLVVEIG"
BASE COUNT          464 a         1236 c         1244 g          603 t
ORIGIN      
        1 gtcgatgagc gcctgggagc acggtccggt gcgaccggtg tggttgcagg gctcgagagt
       61 gacgacggcg gtggccccgc gggcctgacc gcgggagagc ttcgagagcg cgtcgacctc
      121 ggcgtgcggg gtgccggaac cgcggtgcca gccctcggcg atcacctctc cggcggcgga
      181 gaggatcacg gcgccgacct gcgggttcag cccgcgcggg ccgttcagcg ccagcgccag
      241 ggcgcgcgtc atcgcctcgc gttccgcctc gctggatgcc atcggtcgtc tctctctgga
      301 ttcccggggt ccggcggcac gctcgacgcg tcgcccgtgc tgcctctcct ccggactagc
      361 gagctgctgc tcgcatcacc gtcggttccg gagttccacc ggatcggcgc ctgtgaccag
      421 gcgttcgcgg actgtcaccg ccggttcgga ttcacaccga ccccggagca cgttgatgct
      481 ctgagtctag tcaacgcggc atccgatgat tcattcccgg taatacaact cacttcagca
      541 ggcggtcgtg ttctgctcgg tgacgatgcg aggaggaaaa agccggtgtg aacgctgcgt
      601 ggccgacatt ttgctcctcg cgtaccgatc ggggcgtgga ggctcccggt ttcagggacg
      661 gagaagggca cgcacccgac cgtgccggtg cgcgtcctga acgccatggc gatctacttc
      721 agcaggcggg agagccgacg gtcggcgagg ggcttgccgc cggtctggca ggtggggcag
      781 tactccaggc tgcggtcggc gaagaaaacg ctgcgcacgg tgtcgccgca gaccgggcag
      841 gcctcgccac ggcgcgcgtg cacccggaag ccggcgcgct tggcatcctt cagctccgcc
      901 gggggcttgc ccgaggccac ccggaccgcc tcgcgcagcg tgtcctgcat ggccgtgtag
      961 aggcggtcga tcgcgtcgtc gtcgaggttg cccgccgggg cgtacggcga catcttcgcg
     1021 acatgcagga tctcgtcgct gtaggcgttc ccgatgcccg cgatcacgcc ctgatcgcgc
     1081 agcaggccct tgatctgcat ccgccttccc tcgagcagcg atgcgaagac gtcgcgcgtg
     1141 aacgacggat ccagaggatc ggggccgagc cgggcgatgc cgggcacctc ctgtgggtcg
     1201 cgcacgacgt acaccgcgag cgacttcttc gtgccggcct cggtgaggtc gaacccgctg
     1261 ccgtcgtcca gcgcgatccg cagcgcgatc gggctgcgcc cgggcttgat cagcgtcgtc
     1321 ggcagtgcgt cgtaccagcg cagccagccc gccttcgcca ggtggaagac gaggtgaagg
     1381 tcatcgccgc aggcgatgtc gacgaatttg ccgtacctcg cggtggccgt gatggtcgct
     1441 ccatgcagcg cggtgttcgg gggatcgtag gtcttcagcg ccgcgatctc tcccacgctc
     1501 gtgcgcgtga tcgcgtgtcc caccgcgcgc tcgccgagga agtcgacgag cccctgcacc
     1561 tccggcatct cgggcatgca gacatcctgt catccgcatc cgacaacggc cagcacctca
     1621 gagcaccggc cactccacgg gagtgcggtc gtcggatgcc aggagtccgg cgatccaggc
     1681 gtcgtcgcgg ccgcgggggc tggagtgccc ctggcggagc aagccctcat accggaagcc
     1741 gagagctctg gccgtgcgtg ccgacgggat gttgccgacc acagcgcgcc actccactcg
     1801 ggcaagcccc agctctccga acccgaagtc cacgacggca cgcgcggcct cggtcatgac
     1861 accctgaccg cgctgctccg cgacacccca gaatccgatc tcggcctcac cgccgtccgc
     1921 gcgctgcgtg atcttgtgca gcccgatcat tccggcgaac gcaccgtccc ggcggatcgc
     1981 ccagaccgtc tccactccgt ccgcccacca cgaggccacg agcgcgacga agtcctccgc
     2041 gtcctgtctc gagtacgggc tcggcaccgt cgtccagcgc ggcacctcgg gatcctgaca
     2101 ggccgcggtg atcgcgtcga cgtccgcctc ggtcgggatg ctgaggacga ggcgttcggt
     2161 gcgcaatgtg acgggttcca tccgcccagc gtacggcccg gtcggtacgc tggtggcacg
     2221 gccgttccgc cgtcagaccg gggaggacgg ccgtgccgat gcagcgctca tcgcccaagg
     2281 gcgtgctggt gtccctgctg gcatccgtgc tcttcgcgag catcttctac ctgacagtgc
     2341 aactgagctc gtccgcggag gtcgtctggg cctggcgggt gttcgtgacg ctggcgctct
     2401 atctcctggc gctgtggcac cccggggcgc gggaggcgct gcgggtgctg atccgcaggc
     2461 ttcgcgcccg gtggtggatg tcgctgctcg ccctgctgct cgcctcgatc gtgggattcc
     2521 agctgtggct gttcatgtgg gcgcccatgc acgggcacgg actggacgcc tcgctcggct
     2581 acctgctgct gccgatcacg ctcgtgctcg gcgggcgctt catcctgcac gcaccggtga
     2641 gccgggtgca gtggtgcgtg gtcgcgctcg ccctgctggc cgtggcggtg aagttcgcgg
     2701 cgacgccgca gctgtcgtgg gtgaccatcg tcatctgcat cccgtacgcc gtgtacttcg
     2761 tgctgcggca gcgcttcggg ctcgacggac cgctcgtgtt cggcatggag ctggccgtca
     2821 tgtcgccggt cgccgcagtc gtgttcctca cggcgacgcc ggggccgctg ccgatcgcag
     2881 agctcggcgg cctggccctg gtgggcctgc tcggcgggat cgggatggtc ctgtacctgg
     2941 gcgcgtcgac catgctcacc atgccggtgt tcggcctgct cacctacgtc gagccggtgc
     3001 tgctggtcgg cgtcgcgttc gtgctcggcg agcggatgca gggagcggat gccgtcgtct
     3061 acctgatcct tgccgtggcg ctcgcgctgc tgggcgtcga gggcttccgg gcggcgcggc
     3121 ggcgtacgac agatcccgat gtcggcgcgg ccgaatagta tgttccggtg actgttccgg
     3181 ggattctgcg cgccttggaa gaggcgaacc tctatcgtga cgccctcacc tgggcgcaga
     3241 ccgacggcga gatctcggtg gtcgacgggc tcgacgcccc ggtcatcgcg ggcctgctgc
     3301 gcaagcgcgc gcaggcgggg catccggcat ccgccctcgt cgtgacgccg accggccgcc
     3361 gcgccgagtc ggtcgccgca gctctcagca cctatctcgc ggatgccgag atcctgacct
     3421 tccccgcctg ggagaccctg ccgcacgagc gcctgagccc cagccccgac accgtggggc
     3481 gccggctgga gacgctgcgc cggatccgca cctggagcgg cgagaagcca ctggtcgtcg
     3541 agatcgg
//
LOCUS       DATMSX010000080         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124235,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000080 DATMSX010000000
VERSION     DATMSX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124235"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..2217)
                     /locus_tag="VN041_02485"
     CDS             complement(253..2217)
                     /locus_tag="VN041_02485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HWU27943.1"
                     /translation="MTDVLPSGLAISEFDSDIRPQDDLYRHVNGTWLESAEIPADKAR
                     WGSFHLLAEQAEKDVRAIIEESQDAEPGTLNRKVGDLYTSFMDTERIAALGLTPLHEQ
                     LAKVDSIDSIGSFLATVGAFDREGVAALIGVFIEPDPGNPERYVPFALQAGLSLPDES
                     YYRQDSFEGTRTAYRAHLERILTLAGVADAAGTADRAFGLEHEIAGHHWDNVASRDAV
                     KTYNLKTWDEFQTLVGVDLTPWRDAVSVANPTAFDELVVYQPSFFEGLGGLLVEERLD
                     DWKAWLRAKVVHGLAGFLTDDIIDENFSFYGTELTGTPSIRERWKRGVSLVEGSLGDA
                     VGKIYVERHFPAASKVAMDELVANLIEAYRQSIQKLEWMSAETREKALAKLATFRPKI
                     GHPDVWRDYSAVEIDPSDLIGNARRATVFEHDRQVSRIGGPIDRDEWHMPPQMVNAYY
                     NPLMNEIVFPAAILQYPFFDPNRDAAANYGGIGAVIGHEIGHGFDDQGSRYDGDGRLQ
                     DWWTDADREAFEERTKALIAQYDALVPVGLSAENHVNGALTIGENIGDLGGLGIGLKA
                     YELSLGGAEAPVIDGYTGVQRLLLSWAQVWQQKSRDAETIRLLTIDPHSPNEFRCNQI
                     LSNIDAFYEAFDVTEGDALHLPEDQRVTIW"
     gene            complement(2287..2922)
                     /locus_tag="VN041_02490"
     CDS             complement(2287..2922)
                     /locus_tag="VN041_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWU27944.1"
                     /translation="MSTPETRTRTRKSPHQRSAEIHSAAVEIARESGLSALTLRAVAA
                     RAGVAPGLVAHYADSMDDLVARAFRELAASELEQVRAEVALVEDPPARLARMIATVLA
                     SDHDDITLVWVDAWSLGRGSAALGAAIDEQMGAWQAFISEIIEDGRTGGLFRTDAPDA
                     VAWQILAMIDGISAHALTRGTDAAAFAMRLAQACETLVGAEPGVIVARIAR"
     gene            3009..4427
                     /locus_tag="VN041_02495"
     CDS             3009..4427
                     /locus_tag="VN041_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWU27945.1"
                     /translation="MTLTSTPAGLHRNLRQRHVVFIGLAYMSPFAVFDTFGIVSEATN
                     GHVPMSYIIITIAVLFTAFSYARMVRVYPSAGSAYTYTRRTIAAPVGFLVGWASLLDY
                     LFLPMINALLAKIYLSAEFPGVPDWMWIVGLILVITVLNIIGVRVSAVANMIMVVFQA
                     LLAIVFVVLTVRAILSGDALAFTFAPFYSVSADMASLVGGASVLALAFLGFDAVTTLS
                     EETVDPRRTVPRSIMWIAALGAAFFVSITYVMQTLVPDLDTLNAITPDIEGASPYIAL
                     FIGGILFQTIFLAGATVSVVSSGLASQLSASRLLYAMGRDGIIARKLFGYVHPRLGTP
                     VINIVLVGAIALTALQLDLNAATSLINFGAFTAFAFVNLSVIAFWIRHRRTEHTHGSS
                     IGWTLIPGIGFLINVWLWLSLDQLAMTVGLIWSGIGALYLLWLTRGFRRPTPDVAFEE
                     AEDLPTGVVRVGDERAVMSEPR"
     gene            complement(4436..>5592)
                     /locus_tag="VN041_02500"
     CDS             complement(4436..>5592)
                     /locus_tag="VN041_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWU27946.1"
                     /translation="AGAVVTSIALRGGTPVVDDFYAAPRTVPEEPGELIRAEPFTRGV
                     PKDAVGWRILYTTTHGDGSAAVASGIVVAPKTEGEHPAIAWHHGTTGFAQACAPSLAS
                     DPFGSGAMFVVDDIVRNGWALVATDYIGLGTEGPHPYLIGQDSARAELDAVRAARQLD
                     GARLADQTVAWGHSQGGGAALWSTAIAEDYAPDVPLSGTVALAPASDLNGLVEHLPEV
                     TGGSVFASFVISAYSDIYPDVAFDEYIRAGADVTVRQMAGRCLAAPDMLVSVLAVLAL
                     SDDPNILAQSPNTGPLGSRLTKNIPPAPPADIPVLLGQGGADTLVDPDMQLLYAAKLC
                     ATGGDVDFRDYGGYGHVDLVEAHSPAIADALDWTADRLAGKAAPRACDQG"
BASE COUNT          783 a         1866 c         2005 g          938 t
ORIGIN      
        1 agccgcccgt cgcggtgtgc gccggccgcc gcgctgcgac cgatgcgatg cggtggcgac
       61 cccatcggcg ggcacgcgcg gtgtgccacg cgaggggtca ggtaccgaag cggaggggga
      121 gctcacgcag gattcctcac gggtggagta gtcatcggtg accggagatc cgtgcggggg
      181 gagcgggatc ttcgaccgac tgggctatcg taaccccgcc gcccggctga gcggacggcg
      241 gggtcgaagc attcaccaga tcgtgacgcg ctggtcttcc ggaagatgca gggcgtcccc
      301 ctcggtcacg tcgaacgcct cgtagaacgc gtcgatgttg ctgaggatct ggttgcagcg
      361 gaactcgttc ggcgagtgcg ggtcgatcgt cagcagacgg atcgtctcgg cgtctcggct
      421 cttctgctgc cacacctgcg cccagctcag cagcaggcgc tggacgccgg tgtagccgtc
      481 gatcaccggt gcctcggcgc cgccgagcga cagctcgtac gccttcagcc cgatgccgag
      541 cccgcccagg tcgccgatgt tctcgccgat ggtgagagcc ccgttcacgt ggttctcggc
      601 actcagtccg accggcacca gcgcgtcgta ctgcgcgatg agcgccttgg tgcgctcctc
      661 gaacgcctcg cggtcggcat ccgtccacca gtcctgcagg cggccgtcac cgtcgtagcg
      721 gcttccctgg tcgtcgaacc cgtggccgat ctcgtggccg atgaccgcgc cgatgccgcc
      781 gtagttggcg gccgcgtcac ggttcgggtc gaagaacggg tactgcagga tcgccgccgg
      841 gaacacgatc tcgttcatca gcgggttgta gtacgcgttg accatctgcg gcggcatgtg
      901 ccattcgtcg cgatcgatcg gaccgccgat gcgggagacc tggcggtcgt gctcgaagac
      961 ggtggcgcga cgggcgttgc cgatcaggtc ggaggggtcg atctcgacgg cggagtagtc
     1021 gcgccatacg tccgggtggc cgatcttcgg gcggaaggtg gcgagcttgg ccagcgcctt
     1081 ctcgcgggtc tccgcgctca tccactcgag cttctgtatg ctctgccggt aggcctcgat
     1141 gaggttggcg acgagctcgt ccatcgcgac cttggatgcc gcggggaagt gccgctcgac
     1201 gtagatcttg ccgaccgcgt cgcccagcga gccctcgacc agcgagacgc cgcgcttcca
     1261 gcgctcgcgg atcgacgggg ttccggtgag ctcggtgccg tagaacgaga agttctcgtc
     1321 gatgatgtcg tcggtgagga agccggccag cccgtgcacg accttggcgc gcagccaggc
     1381 cttccagtcg tccagccgct cctcgaccag cagaccgccc aggccctcga agaagctggg
     1441 ctggtagacg acgagctcgt cgaaggcggt ggggttggcc accgacacgg cgtcccgcca
     1501 cggggtgaga tccacgccga ccagcgtctg gaactcgtcc caggtcttca ggttgtaggt
     1561 cttcacggcg tcgcgcgagg cgacgttgtc ccagtggtgc ccggcgatct cgtgctcgag
     1621 gccgaaagcg cggtccgcgg tgcccgccgc gtccgcgacg ccggcgaggg tcaggatgcg
     1681 ctcgaggtgg gcgcggtatg cggtgcgggt gccctcgaag ctgtcctggc ggtagtagct
     1741 ctcgtcgggc agcgacagac cggcctgcag agcgaacggc acatagcgct cggggttgcc
     1801 cgggtcgggc tcgatgaaca cgccgatgag cgcggcgacg ccctcgcggt cgaacgcgcc
     1861 gaccgtggcg aggaaggaac cgatgctgtc gatcgagtcg accttcgcca gctgctcgtg
     1921 cagcggcgtg aggccgagcg cggcgatgcg ctcggtgtcc atgaagctgg tgtacaggtc
     1981 gcccaccttg cggttcagtg tgccgggctc ggcgtcctgc gactcctcga tgatcgcgcg
     2041 cacgtccttc tcggcctgct cggcgagcag gtggaaggag ccccagcgcg ccttgtcggc
     2101 ggggatctcg gccgactcca gccacgtgcc gttgacgtgg cggtagaggt cgtcctgggg
     2161 gcggatgtcg gagtcgaact cgctgatggc gagacccgag ggcagaacgt cagtcatgcg
     2221 gtccagctta ggcatccgcg cggacatcga agaagacccg gcgccggatg gtgcgatgtc
     2281 tcgcgctcag cgcgcgatac gcgcgacgat cacacccggc tccgccccca ccagcgtctc
     2341 gcatgcctgc gcgagacgca tcgcgaaggc cgcggcgtcg gtgccgcggg tgagcgcgtg
     2401 cgcggagatg ccgtcgatca tggcgaggat ctgccaggcg acggcatccg gtgcatcggt
     2461 gcggaagagc ccgccggtgc ggccgtcctc gatgatctcc gagatgaacg cctgccaggc
     2521 gcccatctgc tcgtcgatgg cggcgccgag cgccgcgctc ccgcgaccca gcgaccaggc
     2581 gtccacccac accagcgtga tgtcgtcgtg atcgctggcg agcaccgtcg cgatcatgcg
     2641 cgccagccgg gccggcggat cctcgacgag cgcgacctcg gccctcacct gctcgagctc
     2701 ggacgccgcg agctcgcgga atgctcgcgc gaccaggtcg tccatactgt cggcgtagtg
     2761 cgcgaccagc cccggggcga cgcctgcgcg cgcggcgacg gctcgcagcg tcagcgccga
     2821 caggccgctc tcgcgggcga tctcgacggc ggcgctgtgg atctccgccg agcgctggtg
     2881 cgggctcttg cgcgtgcggg tgcgggtttc gggcgttgac atggtgactc cagtgtagga
     2941 acattgcgta ttgatcgctt gatcaatacg gcttcactcg acggccgcac gattacaagg
     3001 aggtaatcat gaccctcacg agcaccccgg cgggactgca tcgcaacctc aggcagcggc
     3061 acgtcgtctt catcgggctg gcgtacatgt cgccgttcgc cgtgttcgac acgttcggca
     3121 tcgtgtccga ggcgacgaac gggcacgtgc ccatgtcgta catcatcatc acgatcgccg
     3181 tgctgttcac cgcgttcagt tacgccagga tggtgcgcgt ctacccgtct gcgggctccg
     3241 cctacacgta cacgcgtcgc acgatcgccg ctccggtggg attcctggtc ggctgggcgt
     3301 cgctgctgga ctacctgttc ctgccgatga tcaacgcgct gctggcgaag atctacctct
     3361 cggcggagtt ccccggggtg ccggactgga tgtggatcgt cggcctgatc ctggtgatca
     3421 cggtgctgaa catcatcggc gtgcgcgtca gcgcggtggc gaacatgatc atggtcgtgt
     3481 tccaggcgct cctggcgatc gtgttcgtcg tcctgacggt gcgggcgatc ctctccggcg
     3541 acgcgctggc gttcacgttc gcgccgttct actcggtgag tgccgacatg gcgagcctgg
     3601 tcggcggcgc ctcggtgctg gcgctggcgt tcctgggctt cgacgcggtc accacgctgt
     3661 cggaggagac cgtcgacccg cgtcgcaccg tgccgcgctc gatcatgtgg atcgcagccc
     3721 tcggcgctgc attcttcgtc tcgatcacct atgtgatgca gaccctggtg cccgacctcg
     3781 acaccctgaa cgccatcacc cccgacatcg agggcgcctc gccgtacatc gcgctgttca
     3841 tcggcggcat cctgttccag acgatcttcc tggccggcgc gacggtgtcg gtggtctcct
     3901 ccggcctggc ctcgcaactg agcgcctcgc gcctgctgta tgcgatgggc cgcgacggca
     3961 tcatcgcccg caagctgttc ggctacgtgc acccgcggct gggcacgccc gtgatcaaca
     4021 tcgtgctggt gggcgcgatc gcgctgaccg cgctgcagct cgacctgaac gcggcgacct
     4081 cgctgatcaa cttcggcgcc ttcacggcgt tcgcgttcgt gaacctgtcg gtgatcgcgt
     4141 tctggatccg ccaccgccgc accgagcaca cgcacgggtc gtcgatcggg tggacgctga
     4201 tcccgggcat cgggttcctg atcaacgtgt ggctgtggct gagcctggac cagctggcga
     4261 tgaccgtcgg cctgatctgg tcgggcatcg gcgcgctcta cctgctgtgg ctgacgcggg
     4321 gcttccgccg gccgacgccg gacgtggcgt tcgaggaggc cgaggacctg ccgacgggcg
     4381 tggtgcgcgt gggggatgag cgggcggtca tgtccgagcc ccggtagcgg cggactcagc
     4441 cctggtcgca ggcgcgcgga gcggccttcc ccgccagccg gtctgcggtc cagtcgagcg
     4501 catccgcgat ggccggggag tgcgcctcga cgaggtcgac gtgaccatac ccgccgtagt
     4561 cacggaagtc gacgtcgcca cccgtcgcac acagcttcgc ggcgtagagc aactgcatgt
     4621 cgggatcgac gagggtgtcc gcgccgccct gaccgagcag taccgggatg tcggcgggcg
     4681 gcgccggcgg gatgttcttg gtcaggcggc tgccgagggg gccggtgttc gggctctggg
     4741 cgaggatgtt ggggtcatcg gagagtgcga gcaccgcgag cacggagacc agcatgtcgg
     4801 gcgcggccag gcagcggcct gccatctgcc gcacggtgac atcggcgccg gcacggatgt
     4861 actcgtcgaa cgccacgtcc gggtagatgt cgctgtacgc ggagatcacg aacgaggcga
     4921 agacgctgcc gcccgtcacc tcgggaagat gctcgacgag cccgttcagg tcgctggccg
     4981 gggccagggc caccgtgccc gagagaggga cgtcgggcgc gtaatcctcg gcgatggcgg
     5041 tcgaccacag cgcggcgccg cctccctgcg agtgccccca ggcgaccgtc tggtccgcca
     5101 ggcgggcacc gtccagctgc cgggcggcgc gcaccgcgtc gagctcggcg cgggcgctgt
     5161 cctggccgat caggtacggg tggggtcctt cggtgccgag gccgatgtag tcggtcgcga
     5221 ccagagccca gccgttgcgg acgatgtcgt cgacgacgaa catcgccccg gatccgaagg
     5281 ggtcggatgc cagcgacggc gcgcacgcct gggcgaagcc cgtggttccg tggtgccagg
     5341 cgatggcggg gtgctcgccc tcggtcttcg gcgcgaccac gatgccggag gcgacggccg
     5401 cgctgccgtc gccgtgggtg gtcgtgtaga ggatgcgcca gcccacggca tccttcggaa
     5461 caccccgcgt gaacggctca gcacggatca gctcgcccgg ctcctccggc acggtgcggg
     5521 gtgcggcgta gaagtcgtcg acgaccggcg ttccgccgcg cagcgcgatg ctcgtgacga
     5581 cggcgccggc gg
//
LOCUS       DATMSX010000081         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39066,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000081 DATMSX010000000
VERSION     DATMSX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39066"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1538)
                     /locus_tag="VN041_02505"
     CDS             complement(69..1538)
                     /locus_tag="VN041_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HWU27947.1"
                     /translation="MTSSPDTAETTPPERGFFGQPRALANLFGVEMWERFSFYGMQGI
                     LLIYLYFSVAKGGLGMPEAIAGGIVGAYGGSVYLSTILGAWLADRLLGSERVLFFSAM
                     VIVAGHAALALLPGFVGVGVGLVLIALGSGGLKANATSVVGTLYSEEDTRRDAGFSLF
                     YLGINLGSFFGPVLTGLLQSTVGFHWGFGLAAVGMTAGLIQYSFGRRNLPAAASAVPN
                     PLPARRYPLVIGIAVVAIALIVVLTLLGVIRPENLANIVIVGALVATLAYFTIILSSR
                     HVDATERSRVWAFVPLFITSVAFWSLYQQQFTVLTVYSDKRLDRMILGWEMPLVWVQS
                     INPVFIIVLSGVFAALWTKLGKRQPTTPMKFALGTMIMGGAFLLFLPFANGGEGSTPL
                     LAIVGILLVFTVAELLLSPIGLSAATKLAPSHFKAQMVALFFLSISLGTAISGWLVQF
                     YDPQNEVPYFSILGAIAVVVGLGLALSAKPVLKLMKGVS"
     gene            1609..2946
                     /locus_tag="VN041_02510"
     CDS             1609..2946
                     /locus_tag="VN041_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HWU27948.1"
                     /translation="MSNVAPNDPAAARGISRRAQSDIFRAGISGDRPAVPVDVDALDA
                     EAEAALSKEAYAYIAGGAGSEATVAANRREFGRWQVWPRPLRDVSSRDLSVEFLGRTR
                     PTPLLLAPLGVMELVHPEADLAVARAAASAGVPYTVSNQASFPVERIAQSAPGAPRLF
                     QLYWSASDELNASLLGRAEAAGCEAIVVTLDTHLLGWRTRDLDLACLPFTRGMGIAQY
                     TSDPVFQALVRERAGAAPVDQAARTTDSAVRVTPRTIAAGVSIARKGAGLTGSRSLRE
                     NLRSPLPRTAVETFLDVFSTPTLTWDDLAKAREWTSLPIILKGIVHPDDAERAADAGV
                     DGVWVSNHGGRQIDRSVPTLAVLPEIAERVGGRMPVVFDSGVRSGADAAIALALGADV
                     VALGRPYAYGLGIAGERGVREVIRNVLAELDITLGLAGLTSIGKLDRDALRAV"
     gene            2981..3592
                     /locus_tag="VN041_02515"
     CDS             2981..3592
                     /locus_tag="VN041_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27949.1"
                     /translation="MTEYEQHPADTAFHARLPIGERMDATPLTEGLPWAFEQITMRPL
                     EPMLVPEQPRGGELEPENCSSCKPSEHTIWQDELWQVRSGWDANGLPFVGAISPREHW
                     LLEDAPHEVLAAYGPLMQRISQAVKSIPGVARCHFARWNDGSAHLHLWALARPAGMMQ
                     GRGAALAFWDEMLPPMPAEMAEEHLRIVADALAAGGGHAFPNR"
     gene            3650..4339
                     /locus_tag="VN041_02520"
     CDS             3650..4339
                     /locus_tag="VN041_02520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU27950.1"
                     /translation="MRSIRELDTVELIIDAQGLLDSIRGANRVIDAGTLRALQQSGNY
                     VVGLFDESEGEERMVGASIAFFGEPGKRTMHSHITALLPEYRGRGWGRELKEHQRQWA
                     FSREVGRITWTFDPLVARNAHFFLTVLGARATGYTVNQYGIFGGGDAGDESDRLDVEW
                     ALADIAKPPASDAVVATVEIPTDVEGMRETDPSAAHEWRLRLRADIEQQLSQGRRLAG
                     FETGRGYLFIE"
     gene            complement(4344..4760)
                     /locus_tag="VN041_02525"
     CDS             complement(4344..4760)
                     /locus_tag="VN041_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU27951.1"
                     /translation="MEQRVSFITLAVADVSRSRAFYVDGLGWEPIFEAEGEVLMLPVA
                     ERLILSLWSVEGFTAEIGEPPASGVAPITLAHNLATEAEVDAVLAEAGALGATVSPAT
                     RRDWGGYSGYFADPDGFRWEVAVNPGDTGAFVLPPA"
     gene            complement(4810..5448)
                     /locus_tag="VN041_02530"
     CDS             complement(4810..5448)
                     /locus_tag="VN041_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU27952.1"
                     /translation="MSDIRIVVADDQQIVRDGLVTVLGLIDDFDVVAEADDGEQAVRA
                     VGMHRPDVVLMDLRMPVLDGIAATIRILADFPATRVLVLTTFDDDESITRALQAGASG
                     YLTKDAGRAQLASAIRAVLDGQRVLGDTVGRRLVERLTAGPSDPPAALRSRFPDLTRR
                     EAEVAVLVAADATNAEIAQRLFISTATVKSHINALFAKLGVRTRAEAVATLR"
     gene            complement(5445..>5862)
                     /locus_tag="VN041_02535"
     CDS             complement(5445..>5862)
                     /locus_tag="VN041_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27953.1"
                     /translation="VLDGTRALDLADLAEQVRHLVTTEQRLGASITADIADLRGSASP
                     ALVQAFGEAAREALTNARKHAGGAAVRLGLGLEDGMLVLEVVNGGADPHAVLAASGSG
                     RGLDGIRARFTRLPGGEADITTDHGAFRLRAKARRS"
BASE COUNT          907 a         2095 c         2053 g          807 t
ORIGIN      
        1 ctcccgcctg cgcccggtgc gccccggcga cccggacggc cgcacgctgg gctgacgagg
       61 cctccgcgtc agctgacgcc cttcatgagc ttcagcacgg gcttggccga caacgccagg
      121 cccagtccga cgacgaccgc gatcgcaccg aggatcgaga agtacggcac ctcgttctgc
      181 gggtcgtaga actgcacgag ccagccggag atggccgtgc ccagcgagat cgacaggaaa
      241 aacagcgcga ccatctgcgc cttgaagtgc gagggcgcga gcttggtcgc cgccgacagg
      301 ccgatcggcg agagcagcag ctcggcgacg gtgaacacca gcaggatgcc gacgatcgcc
      361 agcagcggcg tcgagccctc gcctccgttc gcgaacggca ggaacagcag aaacgcgcca
      421 cccatgatca tggtgcccag cgcgaacttc atcggggtgg tcggctgcct cttgccgagc
      481 ttggtccaca gggcggcgaa caccccggag agcacgatga tgaacaccgg gttgatcgac
      541 tgcacccaca ccaggggcat ctcccagccc aggatcatgc ggtcgaggcg cttgtcggag
      601 tacaccgtga gcacggtgaa ctgctgctgg tacagcgacc agaacgccac gctcgtgatg
      661 aacagcggca cgaacgccca cacccgggag cgctcggtcg cgtcgacgtg acggctggac
      721 aggatgatgg tgaagtacgc cagcgtcgcg accagcgcgc cgacgatgac gatgttcgcg
      781 aggttctcgg ggcggatcac gccgaggagc gtcagcacca cgatcagtgc gatcgccacg
      841 acggcgatgc cgatgaccag cggatagcga cgggccggca gcgggttggg aacggcggag
      901 gccgcggccg gcaggttccg gcgcccgaag ctgtactgga tgaggccagc cgtcatgccg
      961 accgccgcga gtccgaagcc ccagtggaac ccgacggtcg actgcagcag cccggtcagc
     1021 acggggccga agaaggagcc gaggttgatg cccaggtaga acagcgagaa accggcgtcg
     1081 cgccgggtgt cctcctcgga gtacagcgtg ccgaccaccg aggtggcgtt ggccttcagg
     1141 ccgcccgacc cgagggcgat cagcaccaga ccgacgccca cgcccacgaa accggggagg
     1201 agggcgaggg cggcgtgccc ggcgacgatg accatggcgc tgaagaacag cacccgctcc
     1261 gagccgagca ggcgatccgc cagccaggcg cccaggatcg tggacaggta caccgatccg
     1321 ccataggcgc cgacgatgcc accggcgatc gcctcgggca tgcccagtcc gcccttggcg
     1381 acgctgaagt acaggtagat gagcaggatg ccctgcatgc catagaagct gaaccgctcc
     1441 cacatctcga cgccgaacag gttcgcgagc gcgcgcggct gaccgaagaa cccgcgttcc
     1501 gggggcgtcg tctcggcggt gtccggcgaa gaagtcatcc ctccagctta cgtgcaggcc
     1561 gactcccggc cctgtccggg ccgatccgcg gcggctaggg tgggcgccat gagcaacgtg
     1621 gctccgaacg accctgctgc ggcgcgaggc atctcgcgcc gtgctcagtc cgacatcttc
     1681 cgcgccggca tcagcggtga tcgtcccgcc gtcccggtcg acgtcgacgc gctggatgcc
     1741 gaggccgagg ccgccctgtc gaaggaggcg tacgcctaca tcgccggcgg ggcgggctcc
     1801 gaggcgaccg tcgcggcgaa ccgtcgcgag ttcgggcgct ggcaggtatg gccccggccg
     1861 ctgcgggacg tctcctcccg agatctctcc gtggagttcc tcgggcgcac ccgccccacc
     1921 cctctgctgc tcgccccgct cggggtgatg gaactcgtgc accccgaggc agacctcgcc
     1981 gtcgcgcggg cggcggcgtc cgccggcgtg ccgtatacgg tctcgaacca ggcatccttc
     2041 cccgtcgagc gcatcgcgca gtcggcaccc ggcgcacctc ggctgttcca gctgtactgg
     2101 tcggcatccg acgagctgaa cgcgtcgctg ctcggtcgtg ccgaggcggc cggatgcgag
     2161 gcgatcgtcg tcacgctcga cacccacctg ctgggctggc gcacccgcga cctcgatctg
     2221 gcgtgcctgc cgttcacccg cgggatgggt atcgcgcagt acacctcgga tccggtgttc
     2281 caggcgctgg tgcgggagcg cgcgggcgcc gccccggtcg accaggcggc ccggaccacc
     2341 gactccgccg ttcgcgtcac gccgaggacg atcgccgccg gtgtgtcgat cgcccgcaag
     2401 ggcgccggcc tcaccggaag ccgcagcctg cgcgagaatc tgcgctcgcc gctgccgcgc
     2461 accgccgtcg agacgttcct cgacgtgttc tccacgccca ccctcacctg ggatgacctc
     2521 gccaaggcgc gggagtggac ctcgcttccg atcatcctga agggcatcgt gcaccccgac
     2581 gacgcggagc gggcggcgga cgccggggtg gacggcgtct gggtgtccaa tcacggcggc
     2641 cggcagatcg accgatccgt gccgacgctg gccgtactgc cggagatcgc cgagcgggtg
     2701 ggcgggcgga tgccggtggt gttcgactcc ggcgtccgca gcggagcgga tgccgcgatc
     2761 gccctggccc tcggcgccga cgtcgtggcg ctcgggcgcc cgtacgccta cggtctcggg
     2821 atcgcggggg agcggggcgt ccgcgaggtg atccgcaacg tgctggccga gctcgacatc
     2881 acgctcggcc tcgccggact cacctcgatc gggaagctgg accgggacgc gctgcgggcc
     2941 gtgtgatcgc ccggctcgga tgccgtggtt gcatggacgt atgaccgagt acgagcagca
     3001 tccggccgac accgccttcc acgcccgcct gccgatcggc gagcggatgg acgccactcc
     3061 tctcaccgag gggctgccgt gggcgttcga gcagatcacc atgcgcccgc tggagccgat
     3121 gctcgttccc gagcagccgc gcggcggtga gctcgaacca gagaactgct cctcctgcaa
     3181 gccgagcgag cacacgatct ggcaggacga gctgtggcag gtgcggtcgg gctgggacgc
     3241 caacgggctg ccgttcgtcg gggcgatctc cccgcgcgag cactggctgc tcgaggatgc
     3301 cccgcacgag gtgctggcgg cgtacggtcc gctgatgcag cgcatctcgc aggcggtcaa
     3361 gagcatcccc ggcgtcgccc gctgtcactt cgcccgctgg aacgacggct ccgcgcacct
     3421 gcacctgtgg gcgctggcgc ggccggccgg catgatgcag gggcgcggtg ccgcgctcgc
     3481 gttctgggac gagatgctcc cgcccatgcc ggcggagatg gccgaggagc acctgcgcat
     3541 cgtcgccgac gcgctcgccg cgggcggcgg gcacgcgttt ccgaatcgct gagccgctcc
     3601 acggcccgcc gcgtttcgcg ttccgcaccg cgaggggcag actgggttcg tgcgaagcat
     3661 ccgcgaactc gacaccgttg aactgatcat cgacgcccag ggcctgctgg actccatccg
     3721 cggcgccaac cgcgtcatcg acgccggcac gctgcgcgcg ctgcagcagt cgggcaacta
     3781 cgtggtcggg ctcttcgacg aatccgaggg cgaggagcgg atggtgggcg cctcgatcgc
     3841 gttcttcggc gagccgggca agcgcaccat gcactcgcac atcaccgcgc tgctgcccga
     3901 gtaccgcgga cgcggctggg gccgcgagct caaggagcac cagcgccagt gggccttctc
     3961 ccgcgaggtc ggtcgcatca cgtggacctt cgacccgctg gtcgcccgca atgcgcactt
     4021 cttcctcacc gtgctcgggg cgcgggcgac cggctacacg gtcaaccagt acggcatctt
     4081 cggcggcggc gacgcgggcg acgagagcga ccggctggat gtggagtggg ccctggccga
     4141 catcgccaag ccgccggcat ccgacgccgt ggtcgcgacc gtcgagatcc cgacggatgt
     4201 cgagggcatg cgcgagaccg atccgagtgc cgcgcacgag tggcgtctgc gcctgcgcgc
     4261 cgacatcgag cagcagctgt cgcagggtcg tcgtctcgcc gggttcgaga ccggccgcgg
     4321 ctacctcttc atcgagtgac ctgtcacgcc ggcggcagca cgaacgcgcc ggtgtcaccg
     4381 gggttgaccg cgacctccca ccggaagccg tcggggtcgg cgaagtaacc cgagtaccca
     4441 ccccagtcgc gacgggtggc gggtgagacc gtggcgccca gcgccccggc ctcggcgagc
     4501 accgcatcca cctccgcctc ggtggcgagg ttgtgcgcga gggtgatcgg ggcgacgccg
     4561 gatgccggag gctccccgat ctcggcggtg aaaccctcca cgctccacag cgagaggatc
     4621 agccgctcgg cgacggggag catcagcacc tcgccctcgg cctcgaagat cggctcccag
     4681 ccgagcccgt cgacgtagaa cgccctgctg cggctcacgt ccgcgacggc gagggtgatg
     4741 aagctcacgc gctgttccat gcgcaccacc ctgccaccgg cgaccgacag agggaaccgg
     4801 cgacgccgat cagcgaaggg tcgctacggc ttccgccctt gtcctcacac cgagcttcgc
     4861 gaacagcgcg ttgatgtgcg atttcaccgt cgccgtcgag atgaacaggc gctgcgcgat
     4921 ctcggcattc gtggcgtcgg cggcgacgag cacggcgacc tccgcctcgc gtcgggtgag
     4981 gtccgggaat cgactgcgca gtgccgcagg cggatcggac ggacctgcgg tcagtcgctc
     5041 gaccagccgt ctgccgacgg tgtcgccgag cacacgctga ccgtccagca cggcccggat
     5101 ggccgacgcg agctgcgcgc gaccggcatc cttcgtgaga tacccgctcg cgcccgcctg
     5161 cagcgcgcgg gtgatcgact cgtcgtcgtc gaaggtcgtc agcacgagca cccgcgtcgc
     5221 ggggaagtct gcgaggatgc ggatggtcgc cgcgatgccg tcgagaaccg gcatccgcag
     5281 gtccatcagc accacgtccg ggcggtgcat gccgacggcc cggacggcct gctcgccgtc
     5341 gtccgcctcg gcgacgacgt cgaagtcatc gatcagtccg agcaccgtga ccaggccgtc
     5401 gcgcacgatc tgctggtcgt cggcgacgac gatccggatg tcgctcatga gcgcctcgcc
     5461 ttcgcacgta gccggaacgc cccatgatcc gtggtgatgt ccgcttcgcc cccgggcagt
     5521 cgggtgaagc gtgcccggat gccgtccagc ccgcgaccgg agccactcgc ggcgagaaca
     5581 gcgtgggggt cggcgccccc gttcacgacc tcgagcacca gcatcccgtc ctcgagcccg
     5641 agtcccagcc ggactgccgc tccgcccgcg tgcttgcgcg cgttggtgag tgcctctcgg
     5701 gcggcctcac cgaacgcctg gaccagggcg ggggacgccg aaccgcgcag gtcggcgatg
     5761 tccgccgtaa tggacgcacc cagccgctgc tcggtcgtca ccagatgccg cacctgctcc
     5821 gcgaggtctg cgaggtccag cgcgcgcgtg ccgtccagga cg
//
LOCUS       DATMSX010000082         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_95945,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000082 DATMSX010000000
VERSION     DATMSX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95945"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN041_02540"
     CDS             <1..546
                     /locus_tag="VN041_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27954.1"
                     /translation="RAPRGRMLLVGAGAGALVILGGLLWPGGDEDAVMPQAVAADRQA
                     SPSPSASERGQVTQPEEPRPSASDAPVPEQPQPDRDDGDVERAATRLLKAIDGCRAKK
                     DMDCATAVVAGAAQGVLERLGADAAGRDVTLVEDYGDIAVTRLGRTTANGEQMLVLVR
                     EKDGWLVRDVYDVADQPSGGG"
     gene            complement(570..>1548)
                     /locus_tag="VN041_02545"
     CDS             complement(570..>1548)
                     /locus_tag="VN041_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWU27955.1"
                     /translation="AAPAPAAPAAPAPAAPAAEEASDSLYVTPLVRRLASQQGIDLAT
                     VKGTGVGGRIRKEDVLKAAEAKAQAPAAATAPAAPAAAPLEVSPLRGTTQKMSRLRKV
                     VAERAVASMQQTAQLTTFVEVDVTALAAYRTAKKDEFLQKTGDKLSFLPFFTLAAAEA
                     LQAYPIINSTVDGDSIVYPASENISIAVDTERGLLTPVVRDAGTKNIAQIAHEIADLA
                     ARTRDNKLKPDELAGGTFTVTNTGSRGAMFDTPLVFLPQSAILGTGVVFKRPGVVTVD
                     GVDAISVRSYVWLAISYDHRIIDGADAARFLSAIKNRLEAADFAATLGI"
BASE COUNT          226 a          487 c          606 g          229 t
ORIGIN      
        1 cgtgccccgc gcggacggat gctgctggtg ggcgccggtg cgggggcact ggtgattctg
       61 ggcgggctgc tctggccggg cggcgatgag gatgccgtga tgccgcaggc ggtcgccgct
      121 gatcggcagg cttctccgtc cccttcggca tcagagcgcg gacaggtcac gcagcctgag
      181 gagccgcgac cctccgcctc cgacgctccg gtgccggagc agccgcaacc cgaccgcgac
      241 gacggcgatg tcgagagggc ggcgacgcga ctgctgaagg cgatcgacgg atgccgtgcg
      301 aagaaggaca tggactgcgc gaccgcggtc gtggcgggtg ctgcgcaggg cgtgctcgaa
      361 cggctcggag ctgacgccgc ggggcgggac gtgacgctgg tcgaggacta cggcgacatc
      421 gcggtcacgc gactcgggcg tacgacggcg aacggcgagc agatgctcgt cctggtgagg
      481 gagaaagacg gatggctggt ccgcgacgtg tacgacgtcg cggaccagcc atccggtggg
      541 ggctgagcgg gctcagatcc ccggctggat cagatgccca gcgtggccgc gaagtcggcg
      601 gcctccaggc ggttcttgat cgcgctcagg aagcgcgcgg catccgcacc gtcgatgatg
      661 cggtggtcgt acgagatcgc cagccacacg tacgaacgga ccgagatcgc gtccacgccg
      721 tcgaccgtga ccacgccggg gcgcttgaac acgacgcccg tgccgaggat cgcggactgg
      781 ggcaggaaca ccaggggcgt gtcgaacatc gcaccgcgcg aacccgtgtt ggtcaccgtg
      841 aaggtgccgc cggcgagctc gtcgggcttg agcttgttgt cgcgggtgcg cgcggcgaga
      901 tcggcgatct cgtgcgcgat ctgcgcgatg ttcttcgtcc cggcgtcgcg gacgaccggc
      961 gtgagcaggc cgcgctcggt gtccacggcg atcgagatgt tctcggatgc cgggtagacg
     1021 atgctgtcgc cgtcgaccgt cgagttgatg atcgggtacg cctgcagcgc ctcggcagcg
     1081 gcgagggtga agaacggcag gaaggagagc ttgtcgcccg tcttctgcag gaactcgtcc
     1141 ttcttcgcgg tgcggtaggc ggccagggcc gtcacatcga cctcgacgaa ggtggtcagc
     1201 tgagcggtct gctgcatgga ggccacagcg cgctcggcga cgaccttgcg caggcgcgac
     1261 atcttctgcg tggtgccacg cagaggcgag acctcgagcg gggccgccgc aggcgctgcc
     1321 ggtgcggtcg cagcggccgg agcctgcgcc ttggcctcgg cggccttcag cacatcctcc
     1381 ttgcggatgc gtccgccgac gccggtgccc ttcaccgtgg cgaggtcgat gccctgctgg
     1441 gaggcgaggc ggcggaccag gggcgtcacg tagagcgagt cggatgcctc ttccgcagca
     1501 ggtgcggccg gagccggggc ggcgggtgcg gcaggtgccg gagcggca
//
LOCUS       DATMSX010000083         9524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_48514,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000083 DATMSX010000000
VERSION     DATMSX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48514"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VN041_02550"
     CDS             complement(<1..289)
                     /locus_tag="VN041_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU27956.1"
                     /translation="MASILPFPSPRTPRQPEPLWRELLGAELRRLRHERGDTLTETAE
                     AAGVSPQYLSEMERGLKEPSSEMIAAIAGALDSSLLELTSAVAAELRSASTP"
     gene            447..1088
                     /locus_tag="VN041_02555"
     CDS             447..1088
                     /locus_tag="VN041_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWU27957.1"
                     /translation="MPAATRTFTARHVQLTRAALAAVAAVMITFSPDHSASVGLAVFA
                     GFALSTGLVQLLAAIIVHPSGHRWPSVIIAAVSFAAGMFGGIPGLRSEGLFFTLVIAW
                     AAATGLVELIAGIRSKGAEGARDAMLVGGLGLLLALLLALVPAGFVQESVLAGGVTST
                     LTSIIIGVGLFGGYAAIVAVFLGIAGLTPGTKKTADAETGTDADRLAEHGGHA"
     gene            1085..1468
                     /locus_tag="VN041_02560"
     CDS             1085..1468
                     /locus_tag="VN041_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27958.1"
                     /translation="MTDQKPTRREILRPLHLLGIALACGVFAALVTIFSTGGFTARVN
                     SAIANGTYRDLPPFTLGLVVGGICFIVTLLVLAMLMLAVNPADVTKTIDKPVLLDPKQ
                     KRSRGGDAGADAGIDGTEKPGASGA"
     gene            complement(1472..2560)
                     /locus_tag="VN041_02565"
     CDS             complement(1472..2560)
                     /locus_tag="VN041_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWU27959.1"
                     /translation="MRRDRRSARLAVTTASVAAMLALAGCTVSSTPSPSSSEGSSTLA
                     TGLEAPWSIVVLDDGGALISQRGDGKVLEVTASGQLRTAGAVPGVVSGGEAGLNGLAV
                     REIDGIRWLYAYHATEEDNRVVRMPLSRDPGSLRLDAGRVEDVVTGIPRAANHDGGRI
                     AFGPDGMLYIGTGDAGQRERARDRAYLGGKILRVTPDGEPAPGNPFGTAVYSLGHRNV
                     QGLAWDDSGALWASEFGQDTWDELNRIEAGGDYGWPDHEGKAGAADAIDPIAVWKPAD
                     ASPSGIAMVDGTLLIAALRGERVWAFDPSHPETEPWRARFTEEYGRVRDVAAGPGGKL
                     WLLTNNTDGRGQPREGDDRLVQPAFVEG"
     gene            2664..4082
                     /locus_tag="VN041_02570"
     CDS             2664..4082
                     /locus_tag="VN041_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU27960.1"
                     /translation="MSASVDRATVGLRSERGPVLGALMLATGLIAIDATILATAVPSI
                     VRDLGGYTQFPWLFSVYLLAQAVSVPIYAKFADMIGRKPIILLGIALFLLGSVLCGFA
                     GSMLWLIIFRIVQGLGAGAVGPMSMTIVGDIYTVAERAKVQGYLASVWAIASVVGPAL
                     GGIFAQLDAWRWIFFVNIPLCALAGWMLVRRYHEEKQPRRHRIDFGGAALLTIGLTGI
                     ILGLLEGDSAWAWVSVPSAICFGGGAAALVIFAFVERRASEPIIDFSLIGRRLILSTT
                     IVSFGIGALLIGVTSFAPAYLEGSLGIAPLLSGLAVAALTLGWPLAAANVGHLYLRIG
                     FRRTALIGMCTATIAAIGLAVSGPWPNVYLLAGIAFVLGFGLGWASAPVLIAAQASVG
                     WGERGAVTGMNTFARSAGSAVGVAVLGAISNAVIAQGAGPQDPATIISASTWVFIGAA
                     VTAAITLVALLFMPRDPVAVDA"
     gene            complement(4212..4580)
                     /locus_tag="VN041_02575"
     CDS             complement(4212..4580)
                     /locus_tag="VN041_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWU27961.1"
                     /translation="MRSFEVTAARLPQTRIGTRYAVAQVDDFLDEAAAALTCWEGGTP
                     SALHADDIVHKEFRSVALFGAGYDAAAVDDLLDRVAVQLREYEPGAQLRAEQERQDER
                     DVRGMLEKAQQELREKRGEA"
     gene            complement(4591..5973)
                     /gene="purB"
                     /locus_tag="VN041_02580"
     CDS             complement(4591..5973)
                     /gene="purB"
                     /locus_tag="VN041_02580"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWU27962.1"
                     /translation="MTSQPALPPQPLSPLDGRYRASVSGLADFLSEAGLNRARVEVEV
                     EWLIALTDRSLFGTSPLSDADKERLRALYRDFGQAEIDWLAEKEAVTRHDVKAVEYLV
                     RDRLATLGLDAIAELTHFACTSEDINSASYALTVKRAVEGVWLPALDSVIAKLRELAV
                     EHADAAMLSRTHGQPATPSTMGKELAVFAWRLERVRGRIAASEYLAKFSGATGTWSAH
                     LSADPDADWPTISREYIEGLGIDFNVLTTQIESHDWQVELYDHVRHAGGILHNLATDI
                     WTYISLGYFAQIPVAGATGSSTMPHKINPIRFENAEANLELSGALLNSLSQTLVTSRM
                     QRDLTDSTTQRNIGVAFGHSMLALDNLRRGLDAISLSRDVLLDDLDHNWEVLAEAIQT
                     VIRAEVVAGRSTITDPYALLKDLTRGHRVGAAELAEFVEKLEIGDAAKQRLLALTPAT
                     YTGIAEQLAR"
     gene            complement(5970..6515)
                     /locus_tag="VN041_02585"
     CDS             complement(5970..6515)
                     /locus_tag="VN041_02585"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HWU27963.1"
                     /translation="MTDSDPFRVIFVCTGNICRSPMAEVVLRALAEQRGLGTRVLSRS
                     AGTGDWHLGERADQRTLESLSRRGYDGSAHRAKQFSAASFAENDLIVALDRTHERILK
                     AWARTDDDEGKVTLLRAFDPHASSMDVPDPYYAGPAMFDSVLAMIETATRGLFSQLEP
                     AVRAPRTTLRPLRSPDQEDLP"
     gene            6629..6889
                     /locus_tag="VN041_02590"
     CDS             6629..6889
                     /locus_tag="VN041_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27964.1"
                     /translation="MQTITAAESLAGEDLWAVFSALRLLDDAADAVGDAAVEAGRLVD
                     DARWENQGVRALRLVLDEVRRTFAWEHTQLMLLRSRIAREVM"
     gene            6889..8193
                     /locus_tag="VN041_02595"
     CDS             6889..8193
                     /locus_tag="VN041_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27965.1"
                     /translation="MGDGVSITSGGAIEVDSAAFREVGQRLALLAGEMGGSADVVRDA
                     AHMLSATRGSSLPVHLSDLHAHAARLQQLADRAAETAVSTQLMADVFELAELRARQQA
                     LAVNHPDQALALQERIDVLLSSDSRIAGMTDEVVREWADARLEGLGEHPVEKLLQPGL
                     WSTGILLPIVAGVVASGLMKGKGTTPFGTTLKGTAPPVTVAESARVSGTTAPRDLGQV
                     LARIPQGDAQVRIEKRTYTGGRVEYTAYVAGTKTVVPGTKQPWDMGSNWDLYIDREKS
                     ASSAALSAALEEAGAEPGDRVDFVTYSQGGLAANDLAMNGPYETKVVVAVADPSQMSL
                     GDDQMLLEVTHPDDLVGGGLSGGGSFGGTGSPDSFTITRQGSQGLLSNSLDAHFLDNY
                     KETVDEAMKTKDVRFDPYNENLAAQHRELESVELIDYTAVRP"
     gene            complement(8220..8621)
                     /locus_tag="VN041_02600"
     CDS             complement(8220..8621)
                     /locus_tag="VN041_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWU27966.1"
                     /translation="MRFIIRVVINAFAIWVVTLIPVLGVVVRPFAPGETLQLVLTLLA
                     VAAIFALVNSIIGTIIKVVAFPLYILTLGLISFIVNGFLLWLTAWITSGFGWGLSVES
                     FWWGVVAAIIISIINAVFGAILRPQKKRQRS"
     gene            8761..>9524
                     /locus_tag="VN041_02605"
     CDS             8761..>9524
                     /locus_tag="VN041_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU27967.1"
                     /translation="MTEPILPRIRPEIAALPPYRQGKQAGANAFKLSSNENPFEPLPS
                     VLEALTHTTPINRYPDATAGALRARLGEKFGVDADAVHVASGSVSILHQLVLGTSSVG
                     DEVIYAWRSFEAYPSLPLVAGATGIQVPLAPGARHDLDAMADAVTDRTRVIIVCTPNN
                     PTGPIVTSAEFAAFVERVPQDVLIVLDEAYAEFVTAPDAVDGLAERVFEKHPNVVVLR
                     TFSKAFGLAGLRVGYAIGHPRVLDAARTTGIPLSVT"
BASE COUNT         1364 a         3300 c         3353 g         1507 t
ORIGIN      
        1 ccggggtgct cgccgaccgc agctccgccg ccacggcact ggtgagttcg aggagggagc
       61 tgtcgagggc gccggcgatc gccgcgatca tctcgctcga cggctccttc aggccccgtt
      121 ccatctccga caggtactgc ggcgagacgc cggcggcctc cgcggtctcg gtcagggtgt
      181 caccgcgttc gtggcgcagc cggcgcagct cggcgccgag cagctcgcgc cacagcggct
      241 cgggctgacg aggggtgcgg ggcgagggga acggaaggat cgaggccatg gcccacggta
      301 ggcacgggcg ccgtcgatga tccacctctt ccgcccacag cagaacgcgg aggcggtgct
      361 cggatggccg gtcctccgcc gcccgtgaca ggtgggctca tagcctcccg gcgtagactg
      421 gcagggccac cgatccgagg actgtcatgc ccgccgccac ccgcacattc actgcacgtc
      481 acgtgcagct gacccgtgcc gccctggcgg ctgtggccgc cgtcatgatc accttctcgc
      541 ctgaccactc ggcatccgtc ggactggcgg tgttcgccgg attcgcgctc tccaccggtc
      601 tcgttcagct gctcgcggcg atcatcgtgc accccagcgg ccaccgctgg ccgtccgtca
      661 tcatcgccgc cgtctcgttc gcggcgggga tgttcggggg cattccgggg ctgcgctcgg
      721 agggcctgtt cttcaccctc gtgatcgcct gggctgcggc caccggactc gtcgagctca
      781 tcgcgggcat ccgctccaag ggcgccgagg gcgcccggga tgcgatgctc gtcggcggtc
      841 tcgggctgct gctcgccctg ctgctggcgc tggtgccggc cgggttcgtg caggagtcgg
      901 tgctcgccgg cggtgtcacc tctactctga ccagcatcat catcggcgtg gggctgttcg
      961 gcggctatgc cgccatcgtc gcggtcttcc tcggcatcgc ggggctcacc cccggcacga
     1021 agaagacggc ggatgccgag accggcaccg acgcggaccg tctggccgaa catggaggac
     1081 acgcatgacc gaccagaagc cgacgcgacg cgagatcctg cgccccctgc acctgctggg
     1141 catcgccctg gcatgcggcg tcttcgcggc gctggtcacg atcttttcca ccggcggatt
     1201 caccgcgcgc gtgaacagcg ccatcgcgaa cggcacgtat cgtgacctcc cgccgttcac
     1261 cctcggcctc gtggtgggcg ggatctgctt catcgtcacg ctgctggtgc tcgcgatgct
     1321 gatgctcgcc gtgaaccctg ccgacgtcac caagaccatc gacaagccgg tgctcctcga
     1381 ccccaagcag aagaggtccc gtgggggcga tgcgggggcg gatgccggga tcgacggcac
     1441 cgagaagccc ggcgcctccg gcgcctgagg ctcagccctc gacgaaggcc ggctgcacga
     1501 gccggtcatc gccttcccgt ggctgacccc gcccgtcggt gttgttcgtc agcagccaga
     1561 gctttccacc cggacccgcg gcgacgtccc gcacccgccc gtactcctcg gtgaaccgag
     1621 cccgccaggg ttcggtctcc ggatgcgacg ggtcgaacgc ccacacccgt tcaccacgga
     1681 gcgccgcgat gagcagtgtg ccgtccacca tcgcgatgcc gctggggctg gcatccgccg
     1741 gcttccacac ggcgatcgga tcgatggcat ctgcggcccc cgccttgccc tcgtggtcgg
     1801 gccagccgta gtcgccgccc gcctcgatgc ggttcagctc gtcccaggtg tcctgtccga
     1861 actcgctcgc ccacagggcg ccgctgtcgt cccaggcgag gccctgcacg ttgcggtgcc
     1921 cgagggagta caccgcagtg ccgaacgggt tcccgggtgc gggctcgcca tcgggtgtga
     1981 cccgcaggat cttgccgccc aggtatgcgc ggtcccgggc gcgctcgcgc tgtccggcgt
     2041 cgccggtgcc gatgtacagc atcccgtccg gaccgaacgc gatgcgcccg ccgtcgtggt
     2101 tggccgcgcg cgggatgccc gtgaccacgt cctcgacccg gccggcgtcc agcctcagtg
     2161 atcccgggtc ccggctgagc ggcatccgca ccacccggtt gtcctcctcc gtcgcgtggt
     2221 acgcgtagag ccagcggatg ccgtcgatct cccgcaccgc caggccgttc aaccccgcct
     2281 cgccgccgga cacgacgccg ggcacggcgc ccgccgtgcg cagttggccg gaggcggtga
     2341 cctcgagcac cttcccgtcg ccgcgctgcg agatgagggc gccgccgtcg tcgagcacca
     2401 cgatcgacca gggtgcttcc agccccgtgg ccagggtgct cgagccttcc gaagacgacg
     2461 gcgacggtgt cgaggacacc gtgcagcccg cgagggcgag catcgcggcg acgctcgcgg
     2521 tcgtgacggc gaggcgtgcg gagcggcgat ccctgcgcat gtccgcctcc tcgcatccga
     2581 tgtgttccag catccccgag tccggattcg gaaaccagcg cgacatcggg aataccgggt
     2641 atgggggcag acttgaccct gttatgagcg cctctgtcga tcgcgccacc gtcggactgc
     2701 gatccgagcg cggacccgtc ctcggtgcgc tcatgctcgc caccgggctg atcgcgatcg
     2761 atgcgaccat cctcgccacc gcggtgccca gcatcgtgcg cgacctcggc gggtacacgc
     2821 agttcccgtg gctgttctcg gtctacctgc tcgcgcaggc cgtgagcgtg ccgatctacg
     2881 cgaagttcgc cgacatgatc gggcgcaagc cgatcatcct gctcggcatc gcgctgttcc
     2941 tgctcggctc cgtgctgtgc gggttcgccg gcagcatgct gtggctgatc atcttccgca
     3001 tcgtgcaggg gctgggcgcc ggcgcggtcg gacccatgtc gatgacgatc gtcggcgaca
     3061 tctacaccgt ggccgagcgc gccaaggtgc agggctacct ggccagcgtg tgggccatcg
     3121 cctcggtcgt gggacccgcg ctcggcggca tcttcgccca gctggacgcc tggcggtgga
     3181 tcttcttcgt gaacatcccg ctgtgcgcgc tcgccggatg gatgctggtg cgccgctacc
     3241 acgaggagaa gcagccccgt cggcaccgca tcgacttcgg cggtgccgcc ctgctcacga
     3301 tcggcctcac cggcatcatc ctcggactgc tcgaggggga cagcgcgtgg gcgtgggtct
     3361 cggtgccgag cgcgatctgc ttcggcgggg gagcggcggc gctggtgatc ttcgcgttcg
     3421 tggagcgtcg ggcatccgag ccgatcatcg acttctccct gatcggacgg cgcctgatcc
     3481 tcagcacgac gatcgtgtcg ttcgggatcg gggcgctgct gatcggcgtc acgagcttcg
     3541 cgcccgccta cctggagggt tcgctcggca tcgccccgct gctctccggt ctcgccgtcg
     3601 ctgctctcac gctgggctgg ccgctcgccg cggcgaacgt cgggcacctg tacctgcgca
     3661 tcggattccg ccgcaccgcc ctgatcggca tgtgcacggc cacgatcgcg gcgatcgggc
     3721 tggcggtcag cgggccctgg cccaacgtgt acctgctcgc gggtatcgcg ttcgtgctcg
     3781 gcttcggact ggggtgggcg tccgcaccgg tgctgatcgc cgcgcaggcg tcggtcggct
     3841 ggggcgagcg cggcgccgtg accgggatga acacgttcgc ccgttcagcg ggcagcgcgg
     3901 tcggggtggc cgtgctcggt gcgatctcga acgccgtcat cgcgcagggc gcgggccccc
     3961 aggaccccgc gacgatcatc tcggcatcca cctgggtgtt catcggcgcg gcggtgaccg
     4021 ccgcgatcac gctcgtcgcc ctgctcttca tgccgagaga cccggtcgcc gtcgacgcct
     4081 gagccgcgcg gggggacgcg gcgtggatca gagatgctcc cgccgtcgca cgcggggggt
     4141 gcgtttctga tccgacctga gcccggggat gcgtttctga tccgtgctgc gcgtgcaggg
     4201 tccagcgtgc ctcaggcctc gccgcgcttc tcgcggagct cctgctgcgc cttctcgagc
     4261 atcccgcgca catcgcgttc gtcctgccgc tcctgctcgg cccgcagctg tgcgccgggc
     4321 tcgtactcgc gcagctgcac cgcgacgcga tcgagcagat cgtccacggc ggccgcgtcg
     4381 tagccggcgc cgaacagcgc gaccgaccgg aactccttgt gcacgatgtc gtccgcgtgc
     4441 agagcggacg gggtgccgcc ctcccagcac gtcagcgcag cggccgcctc gtcgaggaag
     4501 tcgtcgacct gcgcgaccgc gtaccgggtt ccgatgcgcg tctgcggaag tcgtgccgcg
     4561 gtgacctcga aggaacgcat cgactcggcg tcagcgtgcg agctgctcgg cgatgcccgt
     4621 gtaggtggcc ggggtcaggg cgagcaggcg ttgcttcgcg gcatccccga tctccagctt
     4681 ctcgacgaac tccgcgagct cggccgcacc gacacggtgt ccgcgggtga ggtctttgag
     4741 cagggcgtag gggtcggtga tggtggagcg cccggcgacg acctccgcac ggatcacggt
     4801 ctggatggcc tcggcgagga cctcccagtt gtggtcgaga tcgtccagca gcacgtcgcg
     4861 cgacagcgag atcgcatcca ggccgcggcg caggttgtcc agcgcgagca tcgagtgccc
     4921 gaacgcgacg ccgatgttgc gctgcgtggt cgagtcggtc aggtcgcgct gcatccgcga
     4981 ggtgaccagc gtctgcgaca gcgagttcag cagcgcaccg gacagctcga ggttcgcctc
     5041 ggcgttctcg aaccggatcg ggttgatctt gtgcggcatg gtcgacgacc ctgtcgcgcc
     5101 ggcgacgggg atctgcgcga agtagcccag cgagatgtac gtccagatgt cggtggcgag
     5161 gttgtgcagg atgccgccgg cgtgacgcac gtggtcgtac agctcgacct gccagtcgtg
     5221 cgactcgatc tgggtggtga ggacgttgaa gtcgatgccc agaccctcga tgtactcgcg
     5281 cgagatcgtc ggccagtcgg cgtcggggtc ggcggagagg tgcgccgacc aggtgccggt
     5341 cgcgccggag aacttcgcca ggtactcgga tgctgcgatg cggccgcgca cgcgctccag
     5401 acgccacgcg aagacggcga gctccttgcc catggtcgac ggcgtggccg gctgtccgtg
     5461 cgtgcgggag agcatcgcgg catccgcgtg ctccacggcc agctcacgga gcttggcgat
     5521 caccgagtcc agcgcaggca gccagacgcc ctcgacagca cgcttgacag tcagcgcgta
     5581 ggaggcggag ttgatgtcct cgctggtgca cgcgaagtgc gtgagctcag cgatggcatc
     5641 caggcccagc gtcgccaggc ggtcgcgcac caggtactcg acggccttca catcgtgacg
     5701 ggtgacggcc tccttctcgg cgagccagtc gatctcggcc tgcccgaagt cgcggtacag
     5761 cgcgcgcagg cgctccttgt cggcatccga caggggagag gtgccgaaca gcgagcgatc
     5821 ggtgagggcg atcagccact cgacctcgac ctcgacacgg gcgcggttga ggccggcctc
     5881 ggagaggaag tcggcgagcc cggagacgga ggcgcggtac cggccgtcca gggggctgag
     5941 cggctggggc gggagagcgg gctgagaagt caggggagat cctcctgatc ggggctccgc
     6001 agcgggcgga gcgtcgtacg aggggcgcgc acagccggtt cgagctgcga gaagaggcca
     6061 cgcgtcgcgg tctcaatcat agccagcacc gaatcgaaca tcgccgggcc cgcgtagtac
     6121 gggtcgggca cgtccatgct cgaggcgtgc gggtcgaagg cccgcagcag ggtgaccttg
     6181 ccctcgtcgt cgtcggtgcg cgcccaggcc ttgagaatgc gctcatgcgt gcgatccagg
     6241 gcgacgatca ggtcgttctc cgcgaaggat gctgcgctga actgcttcgc ccggtgggct
     6301 gagccgtcat agccgcgtcg ggagagggac tccagggtgc gctggtcggc gcgctcgccg
     6361 agatgccagt cgccggtgcc tgcgctgcgg gagagcacgc gcgtgccgag gccgcgctgc
     6421 tcggcgagcg cgcggagcac cacctcggcc atcggggatc ggcagatgtt gcccgtgcag
     6481 acgaagatga cgcggaaggg atccgagtcg gtcatgcgtc cattctgccg gtatcgactt
     6541 ctctccccaa tcgtcaggca tggggagttg tccacagcag gtgggtttcc ggccggagcg
     6601 gatgcccgtc ggcgcgcacc ctggaggcat gcagacgatc acggcggccg aaagcctggc
     6661 aggcgaagac ctgtgggcgg tgttctcggc gctcaggctt ctcgacgacg cggcggatgc
     6721 cgtgggggat gccgcggtgg aggcggggcg gctcgtcgac gacgcgcggt gggagaacca
     6781 gggcgtccgg gcgctgcgcc tcgtgctgga cgaggtgcgt cgaaccttcg cctgggagca
     6841 cacgcagctg atgctcctgc ggagccgcat cgcacgggag gtgatgtgat gggcgacggg
     6901 gtgagcatca cctccggcgg agcgatcgag gtcgactccg cggcgttccg tgaggtcggg
     6961 caacggctgg cgctgctcgc gggtgagatg ggtggctcgg cggacgtcgt tcgcgatgcc
     7021 gctcacatgc tgagtgcgac acgcggatct tcgctgccgg tgcatctgtc ggatctccat
     7081 gcgcatgccg cccgcctgca gcagctggcg gaccgtgcgg ccgagacggc ggtgtcgacg
     7141 cagttgatgg cggatgtgtt cgagctcgcc gagctgcgtg ccaggcagca ggcactcgcc
     7201 gtcaaccatc cggatcaggc gctcgccctg caggagcgga tcgacgtgct gctgagttcg
     7261 gactcgcgga tcgccggcat gacggatgag gtcgtccggg agtgggcgga tgcccggttg
     7321 gaggggctgg gggagcatcc ggtcgagaag ctgctgcagc ccgggttgtg gtcgacgggc
     7381 atcctgctcc cgatcgtggc gggggtcgtc gcaagcggac tgatgaaggg caagggcacg
     7441 acaccgttcg ggacgacgct gaagggaacc gcgccgcccg tgacggtcgc cgagtccgcg
     7501 cgggtgagcg gcacgaccgc cccgcgggat ctcgggcagg tgctcgctcg tattcctcag
     7561 ggcgatgcgc aggtcagaat cgagaagcgc acgtacaccg gcggacgcgt cgagtacacc
     7621 gcctacgtcg caggcacgaa gacggtcgtt cccggcacga agcagccctg ggacatgggc
     7681 tcgaactggg atctgtacat cgatcgcgag aagtccgcgt catccgcggc actgtcggct
     7741 gcgctcgagg aagccggcgc cgaaccgggc gaccgcgtcg acttcgtgac ctactcccag
     7801 ggcggcctcg ccgcgaacga cctcgcgatg aacggaccct atgagacgaa ggtcgtcgtc
     7861 gcggtggccg accccagtca gatgtcgctg ggggacgatc agatgctgct ggaggtgacc
     7921 catcccgacg acctcgtcgg cggcgggctc agcggcggcg gttcgttcgg tggcacgggg
     7981 tcgcccgaca gcttcaccat cacccggcag ggctcacagg ggctgctgag caactccctc
     8041 gacgcgcact tcctggacaa ctacaaggag acggtggacg aagcgatgaa gacgaaggac
     8101 gtgcggttcg atccgtacaa cgagaacctc gcggcccagc accgggaact ggagtcagtg
     8161 gagctcatcg actacaccgc ggtgcgcccg tgaccgggca cccgctgcac ggtcccgcgt
     8221 cagctgcgct gtcgcttctt ctgcgggcgc aggatcgcac cgaagacggc gttgatgatc
     8281 gagatgatga tcgcggcgac cacgccccac cagaacgact cgacgctcag accccagccg
     8341 aagccgctgg tgatccaggc cgtcagccac agcaggaagc cgttcacgat gaacgagatg
     8401 agtcccagcg tgaggatgta gagggggaac gcgacgacct tgatgatcgt gccgatgatc
     8461 gagttcacca gggcgaagat cgccgccacg gcgagcaggg tcagcaccag ctgcagggtc
     8521 tcgcccggcg cgaacggccg cacgacgacc ccgagcacgg ggatcagcgt gaccacccag
     8581 atggcgaagg cgttgatgac gacgcggatg atgaatcgca tctgtcgatc gtcccacggg
     8641 atgcgtcgcg gcgggatggc agttcgtcgc agaatctgcg acggccccat cctcagctcc
     8701 gcgcgtcccc gcccatgacg gccgtcgcca cctccggttc cgcctcctta gactgattcc
     8761 gtgaccgagc ccatcctgcc ccgcatccgc cccgagatcg ccgccctgcc gccgtaccgc
     8821 cagggcaagc aggcgggcgc gaacgccttc aagctctcca gcaacgagaa cccgttcgag
     8881 ccgctgccct cggtgctcga ggcgctcacg cacaccaccc cgatcaaccg ctaccccgac
     8941 gccaccgccg gggcgctgcg cgcccgcctg ggcgagaagt tcggcgtcga tgccgacgcc
     9001 gtgcacgtgg catccggctc cgtctcgatc ctgcaccagc tggtgctcgg cacctcctcg
     9061 gtcggtgacg aggtcatcta cgcctggcgc tccttcgagg cgtaccccag ccttccgctg
     9121 gtcgccggcg ccaccggcat ccaggtcccg ctcgcgcccg gcgcacgcca cgacctcgac
     9181 gcgatggcgg atgccgtcac cgaccgcacc cgcgtgatca tcgtctgcac gccgaacaac
     9241 ccgaccggcc cgatcgtcac gagcgccgag ttcgcggcgt tcgtcgagcg cgtgccgcag
     9301 gacgtgctga tcgtcctcga cgaggcgtac gccgagttcg tcaccgcccc ggacgccgtc
     9361 gacggcctcg ccgagcgcgt gttcgagaag cacccgaacg tggtcgtgct gcgcaccttc
     9421 tccaaggcct tcggcctcgc cggcctgcgg gtcggctacg cgatcggcca cccgcgcgtg
     9481 ctcgacgcgg cccgcaccac cggcatcccg ctgtcggtca ccag
//
LOCUS       DATMSX010000084         5850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_171758,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000084 DATMSX010000000
VERSION     DATMSX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171758"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN041_02610"
     CDS             complement(<1..219)
                     /locus_tag="VN041_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Atu1372/SO_1960 family protein"
                     /protein_id="HWU27968.1"
                     /translation="MSIATRLEELGIELPAVAAPVAAYVPAVVHGGVVYTSGQLPFVD
                     GALPKTGKVGAEVDADAAKGYARTCALNA"
     gene            complement(216..440)
                     /locus_tag="VN041_02615"
     CDS             complement(216..440)
                     /locus_tag="VN041_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27969.1"
                     /translation="MTTWEYLTTPLLIHNTAAILNNWGKQGWELVQVVQGPEGGLVAY
                     FKRPVSQDQAANTGLAAAAEAARQFEGDTK"
     gene            522..3116
                     /locus_tag="VN041_02620"
     CDS             522..3116
                     /locus_tag="VN041_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWU27970.1"
                     /translation="MPQKNRTLKGVLGGLLGLVGLSAVAGVLATAAVTPAIAIAGVSG
                     SQALSIFENLPDALNPSTPMEPTVFYATGADGKPFELARFFDQNRVPVAFNQVSPLMY
                     DALLSSEDKSFYEHGGVNLGATVKAVYDNVRGTSSRGASTISQQFVKNVLIQKCEQDV
                     SAGDPKYSEKLNACWNDATNATGAKGVERKLQEMRYAIQIEKDYSKNDILLGYLNIAN
                     FGGQTYGIEAAANYYFGTTAAKLTLDQAATLAGAVQNPNRFRFDKQGGSWTDKTGKAR
                     NSKEDGYADTKFRRNYVLDRMLIDGKITKKQHDDAVALPITPNVHPTTQGCQAAGQQG
                     YFCQYVKNLIEDDKVFGATVGERRDTLRRGGMSVYTSLDMRVQNAGNEAMAARVPTHL
                     DDPNHVIAGSAVTIEASTGRILAIAQNTQFNESANANGTPGQSSLVYAADRDHGNSEG
                     FQPGSSYKVFTLLDWLEKGHSVNETLDGRRSSLLPSSTYETCGKPVTYSGSDNISKNY
                     GSVPGYVGTPMRFTAASLNSGFYAMAKQLDLCDINKVAERLGVHQGNMVPVTKTNTLY
                     ESILGSRNIAPIQMAGAYATIANKGVYCTPKAIDKIVGADGKEMPLPESSCTQVISPE
                     VAATASYALHGVMSATGSAANTRDGIPMFGKTGTHQEIGTAMALSSTKTTTFVYVGKN
                     GGKDFPIAKNFFRGKSLNTARFDIARALERAANQVYGGDALPRPDKNLTRQVLKDLPN
                     VVGMSVEEATKTIEDAGFSVQVGAPVDSTVAAGLVATQTPGAGQAPSGTTVTLSPSTG
                     NPPAAAVPNVVGAKFGQAKKTLEDAGFSVDDKGCNGGSTVTAQNPPGDSPAPPGTTIS
                     IVCDGNPN"
     gene            3113..4051
                     /locus_tag="VN041_02625"
     CDS             3113..4051
                     /locus_tag="VN041_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWU27971.1"
                     /translation="MTSRGSAHPALIALGAVGAAGIAATVWGVGIERYLFTVREVTAP
                     ALAPGSRPVRVLHLSDAHMTPWQHRKQRWLATLATLEPDLVVNTGDNLGHLDGLTGIR
                     RAFEPLAGIPGVFVHGSNDVQAPAPRNPFKYFMGPSGGAKRIQSLDTDAMDEYFTAEL
                     GWRDLNNAAARMEVAGNSIDFLGVDDAHRDWDELEVLPSALGALAERPAGSSVIGVTH
                     APYQRVLNRFVELGADAVFGGHTHGGQVCLPGYGALVANCDIPLKQAKGLSTWTRDGR
                     TVPLNVSAGCGHSIYAPVRFACRPEATLLTLTARSA"
     gene            complement(4167..4742)
                     /locus_tag="VN041_02630"
     CDS             complement(4167..4742)
                     /locus_tag="VN041_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27972.1"
                     /translation="MFSAARFTGTVASLVLAVTALAGCTAASEPAASAPSEKPAAVKT
                     PAGEKPSAPTPIVSTPAPWDPKPPANGCAAEARIATVQNGKDVSGYTEGDGPITFRLE
                     GEPVDRGPRKFAEGTVTLDSAGKVYSYTVAPGDVELTIGDRLCIKSRDFFGYNDILPQ
                     NQPYDPSLTIQPGDVLILQVDPTLEWTPPAA"
     gene            complement(4949..5494)
                     /locus_tag="VN041_02635"
     CDS             complement(4949..5494)
                     /locus_tag="VN041_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27973.1"
                     /translation="MTDNAVRVHVPTRLDRAVTWTGVGAATACAALLMMYGAQSFAAS
                     EPNAENVGEEGIAAALGVFGWTSGLLIAVVAGWALAHRVGRNKPLMRALPLIGGIAWV
                     VLARAATYGQWFERTVSDAFGRFDSLPATLSLQLWPLFVRSVYSVGALPIAVVLLVAF
                     LVLGSVRAFGRRIEPVRDALH"
     gene            5662..5735
                     /locus_tag="VN041_02640"
     tRNA            5662..5735
                     /locus_tag="VN041_02640"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5696..5698,aa:Pro,seq:cgg)
BASE COUNT         1031 a         1961 c         1928 g          930 t
ORIGIN      
        1 ggcgttcagc gcgcaggtgc gggcgtagcc cttggcagca tccgcgtcga cctccgcgcc
       61 gaccttgccg gtcttgggaa gggcgccgtc cacgaacggc agctggccgg aggtgtacac
      121 gacgccgccg tgcacgacgg cggggacgta cgcggccacg ggggcggcga cggcgggaag
      181 ctcgatgccg agctcctcga ggcgggtggc gatgctcact tggtgtctcc ttcgaactga
      241 cgtgccgctt ccgcggcggc ggcgaggccg gtgttggccg cctgatcctg gctgaccggg
      301 cgcttgaagt aggcgaccag accgccctcg ggaccctgca cgacctgcac gagctcccag
      361 ccctgcttgc cccagttgtt gaggatggcc gcggtgttgt ggatcagcag cggcgtggtc
      421 agatactccc acgtggtcat ggcactcctg cgtgtcgtcg gcgcgccgga cggtccgtgc
      481 gtcgaggctt ctattcaggg aactccccta gcatcaagcg tatgcctcaa aagaatcgga
      541 cgctgaaggg tgtgctcggc ggtctgctgg gcctcgtcgg cctgagcgct gtcgccggcg
      601 tgctggccac ggccgccgtc acccccgcca tcgccatcgc cggtgtcagt ggctcgcagg
      661 ccctctcgat cttcgagaac ctgccggatg cgctcaatcc cagcaccccg atggagccca
      721 ccgtcttcta cgcgacaggc gccgacggga agcccttcga gctggcgagg ttcttcgacc
      781 agaaccgcgt gcccgtggcc ttcaatcagg tctccccgct catgtacgat gcactcctct
      841 ccagtgagga caagagcttc tacgagcacg gcggcgtgaa cctgggcgcg accgtcaagg
      901 ccgtctacga caacgtccgc ggcacctcct cccgcggcgc ttcgacgatc agccagcagt
      961 tcgtgaagaa cgtgctcatc cagaagtgcg agcaggatgt ctcggcaggc gatccgaagt
     1021 acagcgagaa gctcaatgcc tgctggaatg acgccaccaa cgccaccggc gccaagggcg
     1081 tcgagcgcaa gctgcaggag atgcgctacg cgatccagat cgagaaggac tactccaaga
     1141 acgacatcct gctcggctac ctgaacatcg ccaacttcgg cggccagacc tacggcatcg
     1201 aagccgctgc gaattactac ttcggcacga ccgccgccaa gctcacgctc gaccaggctg
     1261 ctacgctcgc gggcgccgtg cagaacccga accggttccg cttcgacaag cagggcgggt
     1321 catggaccga caagaccggt aaggcgagga acagcaagga agacggctac gccgacacca
     1381 agttccgccg caactacgtg ctcgatcgca tgctgatcga cggcaagatc accaagaagc
     1441 agcatgatga cgccgtggcg ttgccgatca ccccgaacgt gcacccgacg acccagggct
     1501 gccaggccgc gggccagcag ggctacttct gccagtacgt gaagaatctg atcgaggatg
     1561 acaaggtctt cggcgccacc gtcggcgagc gccgggacac cctccgtcgc ggcggcatga
     1621 gcgtgtacac ctcgctcgac atgcgcgttc agaacgcagg caacgaagcg atggcggcgc
     1681 gggttccgac ccacctcgac gaccccaacc atgtgatcgc gggcagcgca gtcaccatcg
     1741 aggccagcac tggtcgcatt ctcgccatcg cgcagaacac ccagttcaac gaatcggcga
     1801 atgcgaacgg gactcccggg cagagcagct tggtctacgc agcggaccgc gaccacggca
     1861 actcggaagg attccagccg ggttcgtcgt acaaggtctt caccctgctc gactggctgg
     1921 agaagggaca ctcggtcaac gagacgctgg atggacgtcg cagctcgctc ctgccgagtt
     1981 cgacgtacga gacgtgcggc aagcccgtga cctacagtgg cagcgacaac atctcgaaga
     2041 actacggcag cgtccccggc tatgtgggca ccccgatgcg cttcacggcg gcctcgctga
     2101 acagcggctt ctacgcgatg gcgaagcagc tggatctctg cgacatcaac aaggtggccg
     2161 agcggctcgg cgtgcaccag ggcaacatgg ttcccgtcac caagaccaac acgctgtacg
     2221 agtcgattct cggttcgcgt aacatcgcac cgatccagat ggcgggcgcc tatgccacga
     2281 tcgcgaacaa gggcgtctac tgcacgccga aggcaatcga caagatcgtc ggagccgacg
     2341 gcaaggagat gccgctgccg gagtcgtcct gcactcaggt catctctccc gaggtcgcgg
     2401 ccactgcgtc atatgcgctg cacggtgtga tgagcgcgac gggtagcgcg gccaacacga
     2461 gggatggcat ccccatgttc ggcaagaccg gaacccacca ggagatcggc accgcgatgg
     2521 cactgtcgag cacgaagacg accaccttcg tatacgtcgg gaagaacggc ggcaaggact
     2581 tcccgatcgc caagaacttc ttcaggggca agtcgttgaa cacggcgagg ttcgatatcg
     2641 ccagggccct ggagcgcgcc gcgaaccagg tgtacggtgg cgacgcactc ccgcggcccg
     2701 acaagaacct cacgcgtcag gtcttgaagg acctgcccaa cgtcgtcggg atgtccgtcg
     2761 aagaggccac caagacgatc gaggatgccg gattctcagt gcaggtcggc gcacctgtcg
     2821 acagcacggt cgctgccggt cttgtcgcga cgcagactcc cggtgctggt caggctccca
     2881 gcggcaccac cgtgacgctg tcgcccagca ccggcaaccc gcccgcggcg gctgtgccga
     2941 atgtggtcgg cgccaagttc ggacaagcga agaagacgtt ggaagatgct gggttcagcg
     3001 tcgacgacaa gggctgcaac ggcggctcca ctgtgaccgc gcagaatccc cctggcgact
     3061 cacccgctcc cccgggcacg acgatctcga tcgtctgcga cgggaaccct aattgacctc
     3121 gcgaggttcc gcgcacccgg ccctcatcgc gctcggcgcg gtgggggccg cgggcatcgc
     3181 ggcgacggtg tggggcgtcg gcatcgagcg gtacctgttc accgtccgtg aggtgaccgc
     3241 cccggcgctt gctccaggca gtcgtcctgt gcgcgtgctg cacctctcgg atgcccacat
     3301 gacgccgtgg cagcatcgca agcagcgctg gctggcgacg ctcgcgacgc tcgagccgga
     3361 tctggtggtg aacaccggcg acaacctcgg gcatctcgac gggctcaccg gcatccgccg
     3421 tgccttcgag cccctcgcgg ggatccccgg agtattcgtg cacggttcga acgacgtgca
     3481 ggccccggcg ccccgcaatc cgttcaagta cttcatgggc ccctcgggcg gcgcgaagcg
     3541 gatccagtcg ctcgacacgg atgccatgga cgagtacttc acggcggagc tcggctggcg
     3601 cgacctgaac aacgccgcgg cgcggatgga ggtcgccggg aacagcatcg acttcctggg
     3661 ggtcgatgac gcgcatcgcg actgggacga gctggaggtg ctcccctcgg cgctcggcgc
     3721 gctcgcggag cgcccggcag gttcctccgt gatcggcgtg acccacgcgc cgtaccagcg
     3781 cgtcctgaac cgcttcgtgg agctcggtgc ggatgccgtg ttcggcggcc acacgcacgg
     3841 cggccaggtg tgcctcccgg gctacggggc gctggtcgcg aactgcgaca tcccgctgaa
     3901 gcaggccaag ggcctgagca cctggacgcg cgatgggcgc accgtgccgc tgaatgtgag
     3961 cgcgggttgc gggcactcga tctacgcgcc cgtgcgcttc gcctgccgtc ccgaggcgac
     4021 gctgctgact ttgaccgccc gcagcgcctg aacaggcgtc gtgagctgag tctctgcgcg
     4081 tcctgtttcc actattcggg acctataaag tgccgcgcgt ctcatgcgag aaggcagggc
     4141 gttcggtcgg gacttcacgc tgccggtcag gccgctggag gggtccattc gagcgtgggg
     4201 tcgacctgga ggatgagcac gtcgccgggc tgaatcgtga gcgaggggtc gtacggctga
     4261 ttctgcggca ggatgtcgtt gtagccgaag aagtcgcggc ttttgataca caggcggtca
     4321 ccgatggtca attccacgtc gccgggcgcg acggtatagc tgtagacctt gcccgcgctg
     4381 tccagggtca cggtaccctc ggcgaacttc cgcgggccgc ggtcgacggg ctcgccctct
     4441 aggcggaagg tgatcgggcc gtcgccctcg gtataccccg agacgtcctt gccgttctgg
     4501 acggtggcga tccttgcttc tgccgcgcag ccgttggcgg gcggcttcgg atcccagggt
     4561 gcaggcgttg acacgatcgg cgttggtgcg ctcggcttct caccagcggg cgttttcacc
     4621 gcggccggct tctccgacgg cgcggaagca gcgggctcgg atgctgccgt gcacccggcg
     4681 agcgcggtca cggccaggac gaggctcgcg accgtgccgg taaatctcgc tgcgctgaac
     4741 atgccctgat cgtggcatct acgcgggtgg attgccagcc tctgctcgga actcggaact
     4801 tgaccgcagc atcatcgacc gcggaacgac gaggacgtcg tcgaaacgga gttacgcgcc
     4861 ggggtgggtg agaccctgct gacgctgacg gccgcacgtc ttgacggatc gattcggcga
     4921 ccgcgcgaga ctcacctctc caccggcgtc agtgcaacgc gtctctgacg ggttcgatcc
     4981 gccgcccgaa tgcccgcacg ctccccagca cgaggaacgc gacgagcagg acgacggcga
     5041 tcggcagtgc accgacgctg tacaccgacc tgacgaacag cggccaaagc tggaggctca
     5101 gtgtcgccgg cagcgagtcg aaccgtccga acgcgtcgga gaccgtccgt tcgaaccact
     5161 gcccgtacgt cgccgcacgt gcgagaacga cccatgcgat gccgccgatc agtggcaggg
     5221 cgcgcatcag aggcttgttg cgccccactc ggtgggccag cgcccagccg gcgaccactg
     5281 cgatgaggag cccggatgtc caaccgaaga cgccgagcgc cgcggcgatg ccttcctcac
     5341 ctacgttctc cgcgttcggt tccgaggcag cgaacgactg cgcgccgtac atcatcagca
     5401 gtgcagcaca ggcggtcgcc gcgccgacac cggtccaagt gacggccctg tcgagacggg
     5461 tgggcacgtg gacccggact gcattgtcgg tcatgtggcc accataccca gagccacgac
     5521 gaacccgctt cgggcttcat cgtcagagag cgtgtactca tggtgccgct ttgaggctcc
     5581 ggacgctctg gcgctctgat cattgaccgc tcgatttcgt cgttgaggcc gttcgcatgt
     5641 agactcgaac ggttgcaaca acggggtgtg gcgcagcttg gtagcgcgct tcgttcggga
     5701 cgaagaggcc gcaggttcaa atcctgtcac cccgactcaa cgccgcagga cctcgccctc
     5761 agggcggggt cctgcggcgt ttccgtcgct gtgacagagg acccccgccg cgcaggaggc
     5821 tgcgcccgcc gccactcccc gcaacgcgac
//
LOCUS       DATMSX010000085         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_29523,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000085 DATMSX010000000
VERSION     DATMSX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29523"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VN041_02645"
     CDS             complement(<1..258)
                     /locus_tag="VN041_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWU27974.1"
                     /translation="MPKNGKPKGGRPSRNFEPRYAKKTAFHDRHAGGRPARDDDRRTD
                     AGERRPFSSDARADRRPGSISPKHRGYRAAETDAAPKRRWSD"
     gene            514..1416
                     /locus_tag="VN041_02650"
     CDS             514..1416
                     /locus_tag="VN041_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HWU27975.1"
                     /translation="MSSPTGIQIHLSRGDVTAQVAQVGASLRHLTVGGVDIVPPYPED
                     IATPGASGVVLAPWPNRVRDGIWQDGDTTRALAITEPKFHNASHGLLRFTAYEIAVRS
                     EEAVTLAAAIVPQTGYPYLIDTTVRYALTDAGIDVTHTLTNRSAAPAPVALGTHPYLT
                     IGGTDPRTLMLRIPAETYFETDERMLPVGESPVASGTDLREGQRLGDVQLDTGFANLH
                     RDADGFARTTLTAPDGRAVTLQQGPGFGYVQAYTSTGYPDHTLVVAVEPMTAPADAFN
                     SGRGLRHLGQDESWTLEWGIRFSG"
BASE COUNT          224 a          554 c          518 g          220 t
ORIGIN      
        1 atccgaccag cggcgcttgg gcgccgcatc ggtctcagcc gcgcggtagc cgcggtgctt
       61 agggctgatg ctgcccgggc ggcggtccgc acgtgcgtcg gacgagaagg ggcggcgctc
      121 accggcatcc gtccggcggt cgtcgtcacg agcagggcgt ccgccggcgt gacggtcgtg
      181 gaacgccgtc ttcttggcgt agcgcggctc gaagttgcgg gacgggcgtc cgcccttggg
      241 cttgccgttc ttcggcatgg gatcttcctc actgtctctg acacgagaac agcgtccgct
      301 cccgtgcgca cagagatcag cgcacggcgg acatacccgg gcatccgtcg gccggggcca
      361 ttcatgtgat ggttgatgaa ccatcggccc ctgggctcac aatgagagca ggttcaccct
      421 cgcaaaacgc tccgcacgag gcgggtgccc gaagccgacc gcacgagtct accggaccac
      481 cctgggagaa gccctgggtc gtacgattgc ggagtgagct ccccgaccgg catccagatc
      541 cacctcagcc gcggcgacgt gacggcgcag gtcgcgcagg tcggcgcctc gctgcgacat
      601 ctcaccgtcg gcggtgtcga catcgtgccg ccctaccccg aggacatcgc gacgccgggg
      661 gcttcgggag tcgtgctcgc cccgtggccg aaccgcgtcc gcgacgggat ctggcaggac
      721 ggtgacacca cgcgcgcgct ggccatcacc gagccgaagt tccacaacgc cagccacgga
      781 ctgctgcgct tcaccgccta cgagatcgcg gtgcgctcgg aggaggcggt gacgctcgcc
      841 gcggcgatcg tcccgcagac cggctacccg tacctgatcg acacgaccgt gcgctacgcg
      901 ctgacggatg ccggcatcga cgtcacgcac acgctgacca accgctcggc cgctccggca
      961 ccggtcgcgc tcggcactca tccgtacctg acgatcggcg gcaccgaccc gcgcacgctg
     1021 atgctgcgca tccccgcgga gacctacttc gagaccgatg agcggatgct gcctgtcggc
     1081 gaatcccccg tcgcctccgg caccgacctg cgcgaggggc agcgcctcgg cgacgtgcag
     1141 ctggacaccg ggttcgcgaa cctgcaccgg gatgccgacg gcttcgcccg cacgacgctg
     1201 accgcccccg acggccgcgc ggtcacgctg cagcagggcc cgggcttcgg ctacgtgcag
     1261 gcgtacacct cgaccggata tcccgaccac acgctggtcg tcgccgtcga gccgatgacc
     1321 gctcccgccg acgccttcaa ctccgggcgc ggactgcgcc acctgggaca ggacgagtcc
     1381 tggacgctgg agtggggcat ccgcttctcg gggtgattcc gccttccgcc ggcgaccgcc
     1441 tcagtcgacc gggtgcgccg agggcagcgt ctgcgagcgc agctcacggc gggtcgggtg
     1501 gtacgcagcg gggtcg
//
LOCUS       DATMSX010000086        13414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_96015,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000086 DATMSX010000000
VERSION     DATMSX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96015"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VN041_02655"
     CDS             complement(<1..371)
                     /locus_tag="VN041_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27976.1"
                     /translation="MRVGVRVHERRVAAALLHPDGTHTTDEVPVRGDGVEASIDRLLG
                     RIARSAPTVSSIAFDVSDALHLSEVAPLTVVRIAPRPPVAPADEPRVGDAPIIHVRGG
                     HTTLGEELAPFDAAGFRASAAA"
     gene            591..911
                     /locus_tag="VN041_02660"
     CDS             591..911
                     /locus_tag="VN041_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWU27977.1"
                     /translation="MIRSVLTLHTSPAQADSVLELYRREAILQESLDLTRAFSSEIAT
                     AVDGSGEIIVTAVWPDEAAYQEWLDHPNRARTSPELSALLTDARIGVGRLYEVDHRVS
                     RTEI"
     gene            946..1983
                     /locus_tag="VN041_02665"
     CDS             946..1983
                     /locus_tag="VN041_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU27978.1"
                     /translation="MTHLPRIIAAAGVLALGVSLAACSSDGGSGGSGDGDSFSIVFLA
                     SSSQNGYNQAVYEGVQKKAKELQKELGITITTKIQDGQFDANTQLSQLQNAGTTGQAS
                     GVIVVPQDGPGLAAAFPLGNDIPVVSVLNPIGPDIDKMKPQTEGVVSTVASPPSSGAK
                     RQAETVVEYCKDIDPCKIGLLAGLLNSPLDVARIKAWKDVLGAHKNIEIVGTVEGAYD
                     RDKSLTAVSNLLQANKDINGILSNADQQTLGAQIALENAGIEPSSIFLTGGGGTTEAV
                     KAVREGKWTNDYLNYPVSMGEAALEQLINKLRGEKVQAVVDADTLVDLPPMATKEDLE
                     AAPDFTGEWNG"
     gene            1988..3514
                     /locus_tag="VN041_02670"
     CDS             1988..3514
                     /locus_tag="VN041_02670"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWU27979.1"
                     /translation="MQDAASGPVLVELRGVDKRFGATQALDGVSLDVRGGEVHAFVGE
                     NGAGKSTLGKVIAGIYAADGGEVVVDGAPVDRWNPGIAQKQGIAMIAQELALVPDLTV
                     AQNVFLGVEEHRFGIERRNLAARFATLDAQVGFGLDPGEKVRDLRIADQQKVEILRAL
                     ARDARIIVMDEPTSSLTAHEMAQLEQLMRTLRDRGCCVIYVSHFLDSVLGVADRITVM
                     RDGRRIATVDAASVSKHDLVTAMLGREMEQAYPDRPPLPEASDPLLTVCELRTATGVE
                     GVSFEVRPGEIVGLLGLVGSGRTECLRAVFGLDEVVSGEIRFDGRDWTGRTPKHSIDA
                     GLVLVSEDRHKDGLVLQRSVRENIGLASLPARARGGIVDRRSEKRTARELAESLEIRP
                     PDIELPVGWFSGGNQQKALLGKALAADPKLIILDEPTRGVDVGAKRTIYELIVRLAAQ
                     GIGVLLISSEHEEIMELAHRAYLVSNGQTFGEIVPEQTTVEDVLFRLFHVTTEEEAAA
                     "
     gene            3511..4494
                     /locus_tag="VN041_02675"
     CDS             3511..4494
                     /locus_tag="VN041_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU27980.1"
                     /translation="MTEAANTTVTRTRASRKFSPKELVIEYAVLLLLAAVLVALAVLS
                     PSFFTFGNIVNILNQNAPLVIIAAAGTFVIISGNFDLSTGAIYSVAGVVAAWLAVSTG
                     SVALALGAAPVVGILLGAFNGTVITRLRVHSFLATLASSMIFTTVAVLITGGSLITVT
                     TPGFTALGRSRVGGIFIAVFVMIVAVAILWFLLSRTLFGRRVYAVGGNAEAAELSGIH
                     VARTRVLVFVISGLAAGTAAAIGVSRIASGQPLAGAGLELSAIAAVILGGTSIYGGIG
                     AVWRSVAGVFLIALIGNGFDLLNLNPQLKDMVTGFIILAAVGLAAAGRGKR"
     gene            4613..6661
                     /locus_tag="VN041_02680"
     CDS             4613..6661
                     /locus_tag="VN041_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWU27981.1"
                     /translation="MSENVRIAIDVGGTFTDVVKLVPETGELRFEKVPTTPSEPTAGV
                     LAAFGKAEADPADVSTFNHGSTLGLNSLLTRTGARIATIATRGFRDVYLLGRTDRRVM
                     YDIAYRKPEALLERYDTFEVTERCMFDGSVATLFDDEDARGVAREILRRGYDAVAVAF
                     LHAYANPAHELRMREILVEEAPGVEVTLSHELSREYREYERTSTAVLDAYIKPIMRSY
                     LKALEGELERRGFDGRFLMSRSGGGAMTASAAREQPVNLILSGPAGGVVGAAAFAKLI
                     GRPNLITIDMGGTSLDASLVQDGSPVLYHGAEFEGLPINTPSLYIHTIGAGGGSLGYL
                     DEAGALQVGPKSAGAMPGPAAYGRGGTEPTFTDAALAIGYLGTDTPLGGELTLDREKS
                     LESLAPIAGTLGLSVTQLARGMVQISNTKIMGAVRAITIELGHDPKDFALLSFGGGGG
                     LVAVDVARELGIPEVIVPPGQGAFSALGMLMADVQHDYSRTSLQALEDLDAEAADALY
                     SGMEAEARETLRAEGFDDDRMIFHRVVAVRYSGQEHSVTVDYPEDGSDPRAAIRSEFD
                     RLHLRQYGHTMDDPIETTTLRLGAVGVVDKPDLPKAAPRPDGGAPALRTRTVVLDGDE
                     TAEYALVSREELMAGDVLEGPAVVTEHTATTVLHAGDRLEVGAYGELIISIQYAKKGA
                     "
     gene            6663..8369
                     /locus_tag="VN041_02685"
     CDS             6663..8369
                     /locus_tag="VN041_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWU27982.1"
                     /translation="MADAITTEIIRHGLLAAAEEVARNLCRTAYNTVVYEIHDYGIGI
                     HDANGDVVADAPGIAVFTRGNDAGIKHSMEFLGADTMEPGDVFIINYPYWSSAHTLDP
                     LVFAPIHVDDELIGFASCRVHVTDLNQKDPGYVLDSTDRSQEGLMLPASKLYRRGERN
                     EDVFNIIRFNSRMPKHTIGDIQAQVSACVTGVRRTQELADKYGVGTLTEAMVTINEHG
                     EKLARLALAKLPKGTWSAVDYVDDDGINKDELVRLQCTVTVSDEEMVVDWTGSAENVE
                     GPINLPPGQTEALSSLIFKSLTTPDSPVVAGNFAPLRVVTVPGSVMHAVPPMPTFTLW
                     TGLLAGEVILKALAQGMPELVPACSGGDVCSMMGLGVNPRTGEPWQEATNEAVGFGGT
                     ARHDGEDGIMHLSEPGCRNNPVEVLEAKSSMIIEHYGYRADTGGAGEHRGGVGVSRSY
                     RFTAPATGICLVYKTKTEPWAIDGGAVGKPNRVVLNPGTDREVVQGGSYNRLEAGDVL
                     VNQTGGGGGYGDPLRRDPAAVARDVRNGFVSHRSAERDYGVVVTADFGVDETATAAIR
                     GR"
     gene            8459..9649
                     /locus_tag="VN041_02690"
     CDS             8459..9649
                     /locus_tag="VN041_02690"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWU27983.1"
                     /translation="MRTAIGIRPWLPKLKGSDRQESIISLTQVDTTKTTVPAQGRIRQ
                     TYSGTTEFPPMARAYKDVLNVIRDLGLLRRTPVFYTLVGVALLLGFAGCAAGFILLGD
                     SWFQLLIAGALGILFTQVAFLAHEAAHRQILSSGPANFRLARVLAGLIGMSYSWWDSK
                     HTKHHGNPNMVGKDPDIEVDTISFLDEDAARSRGVIRWITQRQGWLFFPLLTLEGLNL
                     HRLSVVHLVTPGRVKGRWTEIGIISLRFALLLTPLFLFLPVGMAFAFLGVQMAVFGVY
                     MGASFAPNHKGMPIISPDARLDFFSKQVRTSRNVTGGWWATWLFGGLNYQVEHHLFPS
                     MSRLNLSRAREVVRDYCAANDVPYTETTVLQSYGIVVRYLNRVGLAARDPFDCPITAQ
                     YRPV"
     gene            9762..11048
                     /locus_tag="VN041_02695"
     CDS             9762..11048
                     /locus_tag="VN041_02695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWU27984.1"
                     /translation="MAAIDDQATQSLLDGRYRLEACVGRGATSMVYRAEDLLLGRTVA
                     IKLLRQSDETPISLERVRTEVALLANLKHPSLVTLYDARLDPGQPHYLAMEYVEGPTL
                     ASRLVSGPLSGAEAASVAHDLAAGLDAVHRAGIIHRDVKPSNVLMPEATRPADHASAK
                     LTDFGIAFDVDDPRRTSPGIAIGTAAYMAPEQVRAADLTTAVDVYSLGLVLIEALTGE
                     PAFPVAGGVQTALRRLTERPAIPESLDAGWRTLLARMTRDDPAERPSAAEVAAEAEAI
                     RRTETSGGGIGTAPPIPTRASLRDVKTNSDDDAPTGQTRAYTAAAVVPPPRFRRTRIA
                     VAAALGAAALSLIAFLGFHGIAPSVESPARIAVHAPQTSPPASPAADPATQQPSTSSS
                     GASSGQGTTTSQPNSNKGPGNNSGKGQGSSGHGKGH"
     gene            complement(11068..12747)
                     /gene="pgi"
                     /locus_tag="VN041_02700"
     CDS             complement(11068..12747)
                     /gene="pgi"
                     /locus_tag="VN041_02700"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWU27985.1"
                     /translation="MTAPIDATTTSAWAELTSLSTGFSPDLRGWFAADPGRVERLSLP
                     LADLHVDLSKNLVTDDILAALVRLAEQTGVAERYAAMLAGEHINTTEDRAVLHTALRR
                     PAGATPALVVDGQDVDADVQSVLDALSAFADRVRSGEWKGVTGKKVTHVVGIGIGGSD
                     LGPVMVYEALKPYADAGIQARFVSNIDPTDLAQKTADLDPETTLFIVASKTFTTLETL
                     TNARLARDWLWAGLSASGAIGDDEQSRTDAVAHHFVAVSTALDKVAAFGIDPANAFGF
                     WDWVGGRYSVDSAIGLSLAVVFGPEVFRDLLAGFHAVDEHVRTTPLAQNVPVLMGLLN
                     VWYTNFLGAQSHAVLPYAQQLSRFAAYLQQLTMESNGKSVRWDGSPVTTTTGEVFWGE
                     PGTNGQHAFYQLIHQGTRLIPADFIAFVNPAYPLQDDGRDVHGLFLANFLAQTKALAF
                     GKTAEEVEAEGTTGALVAARTFPGNRPTTSIFADALTPRVLGELIALYEHITFTQGVI
                     WGINSFDQWGVELGKQLALQIAPAIEGDADALEAQDASTKALLAYYRAHRR"
     gene            12846..>13414
                     /locus_tag="VN041_02705"
     CDS             12846..>13414
                     /locus_tag="VN041_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWU27986.1"
                     /translation="MLEAIPNRSGAIDLVRIIAVAAIVAGHVFTRDLTGDLLYTWHVA
                     VFFVLSGYLWKPGRSFRTELGRRWRSLGRPYLAGFVLLTIALLSMGFPDTLQRLLGGL
                     YGGGVAGMPYITLWFISTLFFTALLLRLIERLPLALQWTLGIAGAVAGWIWGSALSDT
                     PLGVGMAATSLIYLLVGDALRRVRDRVRAP"
BASE COUNT         2145 a         4690 c         4551 g         2028 t
ORIGIN      
        1 gcggcggcgg atgcgcggaa gccggcggcg tcgaacggcg ccagctcttc gccgagcgtc
       61 gtatgaccac cgcgcacatg gatgatcggg gcgtcgccga cccgcggctc atccgcgggc
      121 gcgacgggcg gcctcggtgc gatgcggacc acggtgagag gtgcgacctc cgagaggtgc
      181 agcgcgtcgc tgacgtcgaa agcgatcgag gagaccgtcg gcgcggaccg tgcgatacgt
      241 ccgagcagcc ggtcgatgga cgcctcgact ccgtccccgc gaaccggcac ctcgtcggtc
      301 gtgtgcgtcc cgtcggggtg cagcagcgcg gcggcgacgc gccgctcgtg cactctcact
      361 ccgacacgca tcgtccgctc cttcgcgatc ccctgtccgg acgcaacgcc agagccgccg
      421 cgcatcagtc atttcattct tttgcatttc ttgcggttca cgcaatgtgc attacgcaca
      481 gatgggcggg gtgcgatgcg gataccttca tggagcagcg ccgcggaacc cgacgatgac
      541 agacgcgacg cggcatccca gacgatcgag acaatggagt acgagacccg atgatccgct
      601 cagtcctgac cctgcacaca tcaccggcgc aggcggactc cgtgctggag ctctaccgcc
      661 gcgaggcgat cctgcaggag tcgctcgacc tgacccgcgc cttcagctcg gagatcgcca
      721 ccgcggtcga cggatcgggc gagatcatcg tgaccgccgt gtggcccgac gaggccgcct
      781 accaggagtg gttggaccac ccgaaccgcg cgcgcacctc cccggagctg tcggcgctgc
      841 tcaccgacgc gcggatcggc gtcgggcgcc tctacgaggt cgaccaccgc gtctccagaa
      901 ctgagatctg agtcccaccc gaacccctca cgaaagaaga atgcaatgac gcacctccca
      961 cgtatcatcg ccgccgccgg cgtgctcgcg ctcggcgtct ccctcgcagc ctgcagttcc
     1021 gacggcggat cgggcggaag cggcgacggc gacagcttct cgatcgtctt cctcgcctcc
     1081 tcgtcgcaga acggctacaa ccaggccgtg tacgaggggg tgcagaagaa ggcgaaggag
     1141 ctgcagaagg agctcggcat cacgatcacg accaagatcc aggacggcca gttcgacgcc
     1201 aacacccagc tctcgcagct gcagaacgca gggacgaccg gccaggcgag cggcgtcatc
     1261 gtcgtgcccc aggacggacc gggcctggcc gcggcgttcc cgctgggcaa tgacatcccc
     1321 gtggtgtccg tgctgaaccc catcggaccg gacatcgaca agatgaagcc gcagaccgag
     1381 ggcgtcgtct ccaccgtggc ctcgccgccg tcgtccggcg ccaagcgcca ggcggagacc
     1441 gtcgtcgagt actgcaagga catcgacccc tgcaagatcg gcctgctggc tggactgctg
     1501 aactccccgc tcgacgtcgc acgcatcaag gcctggaagg acgtcctcgg cgcgcacaag
     1561 aacatcgaga tcgtcggcac cgtcgagggc gcctacgacc gcgacaagtc gctcaccgcg
     1621 gtgtcgaatc tgctgcaggc caacaaggac atcaacggca tcctcagcaa cgccgaccag
     1681 cagacgctgg gggcgcagat cgcgctggag aacgccggca tcgaaccctc gtcgatcttc
     1741 ctcaccggcg gaggcgggac gacggaggcc gtcaaggccg tgcgcgaggg gaagtggacg
     1801 aacgactacc tgaactaccc ggtcagcatg ggtgaggccg cgctcgagca gctgatcaac
     1861 aagctgcgcg gcgagaaggt gcaggccgtc gtcgacgccg acacgctcgt cgacctcccg
     1921 ccgatggcga ccaaggagga cctcgaggcg gcgcccgact tcaccggcga gtggaacggc
     1981 tgatccgatg caggatgctg ccagcggacc cgttctcgtc gaactccgcg gagtcgacaa
     2041 gcggttcggc gcgacccagg cgctcgacgg cgtgtcgctc gacgtgagag gcggggaggt
     2101 ccacgccttc gtcggcgaga acggcgcggg taagtcgacc ctcggcaagg tgatcgccgg
     2161 catctacgcg gccgatgggg gcgaggtcgt cgtcgacggc gccccggtcg accggtggaa
     2221 cccggggatc gcacagaagc aggggatcgc gatgatcgcc caggagctgg cgctcgtccc
     2281 cgacctcacc gtcgcccaga acgtcttcct cggtgtcgag gagcaccgct tcggcatcga
     2341 gcggcgcaac ctcgccgcgc gcttcgcgac gctcgacgcg caggtcgggt tcggcctcga
     2401 cccgggggag aaggtgcgcg atctgcgcat cgccgatcag cagaaggtgg agatcctgcg
     2461 ggcgctcgcc agggatgccc ggatcatcgt gatggacgag cccacgtcgt cgctgaccgc
     2521 gcacgagatg gcgcagctcg agcagctgat gcggacgctc cgggacaggg gatgctgcgt
     2581 gatctacgtc tcgcatttcc tcgactcggt cctcggcgtc gccgaccgca tcaccgtgat
     2641 gcgggacggg cggcggatcg cgaccgtcga cgcggcctcg gtgtcgaagc acgatctggt
     2701 gaccgcgatg ctcggccgcg agatggagca ggcgtacccc gaccgtccgc cgctccccga
     2761 agcatccgac ccgctcctca cggtgtgcga gctccgcacc gcgaccggtg tcgagggggt
     2821 gagcttcgag gtgcgccccg gcgagatcgt cggactgctc ggcctcgtgg gcagcgggcg
     2881 gactgagtgc ctccgtgccg tgttcggcct cgacgaggtg gtctccggcg agatccgctt
     2941 cgatggccgc gactggaccg gccggacgcc caagcactcg atcgacgcag gcctcgtgct
     3001 cgtctcggag gaccgccaca aggacggcct cgtcctgcag cgatcggtac gcgagaacat
     3061 cggtctcgcg agtctgccgg cccgcgcgcg aggcgggatc gtcgaccgca ggagcgagaa
     3121 gcgcacggca cgggaactgg cggaatcgct cgagatccgt ccgcccgaca tcgagctgcc
     3181 tgtcggatgg ttctcgggag gcaatcagca gaaggccctg ctcggcaagg cgctcgccgc
     3241 ggacccgaag ctgatcatcc tcgacgaacc gacccgaggc gtcgatgtcg gagcgaagcg
     3301 caccatctac gagctcatcg tcaggctcgc cgcccagggc atcggcgtgc tcctgatctc
     3361 gtcggagcac gaggagatca tggagctcgc ccatcgcgcc tacctggtct ccaacggcca
     3421 gaccttcggg gagatcgtcc ccgagcagac caccgtggag gacgtgctgt tccggctctt
     3481 ccacgtgacc accgaagagg aggcggcagc atgaccgagg ccgcaaacac caccgtgacg
     3541 cgcacccgcg ccagcaggaa gttctcgccg aaggagctcg tcatcgagta cgcggtcctg
     3601 ctcctgctcg ccgccgtgct cgtcgcgctc gccgtgctgt cgccgtcgtt cttcacgttc
     3661 ggcaacatcg tcaacatcct gaaccagaac gcgccgctgg tgatcatcgc cgccgcaggg
     3721 acgttcgtga tcatctcggg caacttcgac ctgtcgaccg gcgcgatcta cagcgtggcc
     3781 ggcgtcgtgg ccgcatggct cgcggtgagc acgggcagcg tcgcgctcgc cctcggcgcg
     3841 gcacccgtcg tcggcatcct cctcggcgcg ttcaacggca ccgtcatcac ccgcctccgg
     3901 gtgcactcgt tcctcgcgac tctcgcctcc tcgatgatct tcacgacggt cgcggtgctc
     3961 atcaccggcg gcagtctgat caccgtgacg acaccgggct tcaccgctct cgggcgcagc
     4021 cgcgtcggcg gcatcttcat cgcggtgttc gtgatgatcg tcgcggtcgc gatcctgtgg
     4081 ttcctgctct cgcggaccct gttcggccgt cgcgtgtacg ccgtcggcgg caacgcggag
     4141 gcggccgagc tgtcgggcat ccacgtggcc cgcacgaggg tccttgtctt cgtgatcagc
     4201 ggtctcgcgg ccggcacggc cgcggcgatc ggggtctcgc gcatcgcgtc cggacagccg
     4261 ctcgccggcg ccgggctcga gctgagtgcg atcgcggccg tgatcctcgg cggcacgagc
     4321 atctacggcg gcatcggagc cgtctggcgc tctgtcgccg gtgtcttcct catcgccctg
     4381 atcggcaacg gcttcgacct gctgaacctg aacccgcagc tgaaggacat ggtgaccggc
     4441 ttcatcatcc tcgcggccgt cggccttgcc gcggccggcc gcggcaagcg ctagcagccc
     4501 tctccacatt ccgccccctg cggcatccgc gacgaacggc ccggtcgacc ccgggtctcc
     4561 gcggcctgcc tggaaaaccc tcaacgaccc accaatacgg aaggaccatg tcatgtccga
     4621 gaacgtccga atcgccatcg acgtcggagg aacgttcacc gacgtggtga aactcgtccc
     4681 cgagaccggc gagctccgct tcgagaaggt gccgaccacg ccgagcgagc cgaccgccgg
     4741 cgtgctggcg gccttcggca aggccgaagc ggaccccgca gatgtctcga ccttcaacca
     4801 cggcagcacc ctgggcctga actccctgct gacccgcacc ggtgcgcgca tcgcgacgat
     4861 cgcgacgcgc ggcttccgcg atgtctatct gctcggccgc accgaccgcc gggtgatgta
     4921 cgacatcgcc tatcgcaagc cggaggctct cctcgagcgg tacgacacct tcgaggtgac
     4981 cgagcgctgc atgttcgacg gctcggtggc gacgctgttc gacgacgagg acgcgcgcgg
     5041 ggtcgcgcgt gagatcctgc gccgagggta cgacgcggtg gcggtcgcgt tcctgcacgc
     5101 ctatgcgaat cccgcgcatg agctccggat gcgcgagatc ctcgtggagg aggcaccggg
     5161 ggtcgaggtc acgctgtcgc acgagctctc ccgcgagtac cgcgagtacg agcgcaccag
     5221 caccgcggtg ctggatgcct acatcaagcc gatcatgcgc agctacctga aggcgctgga
     5281 gggtgagctc gagcggcgcg ggttcgacgg ccgcttcctc atgtcgcgct cgggcggggg
     5341 agccatgacc gcctcggcgg cgcgcgagca gcccgtgaac ctcatcctgt ctggcccggc
     5401 cggcggcgtc gtcggcgccg ccgccttcgc gaagctgatc ggcagaccga acctcatcac
     5461 catcgacatg ggcggcacga gcctcgacgc ctcgctggtg caggacggca gccccgtcct
     5521 ctatcacggc gcggagttcg agggccttcc gatcaacact ccgtcgctct acatccatac
     5581 gatcggcgcg ggcggcggct cgctcggcta cctggacgag gccggcgccc tgcaggtcgg
     5641 gccgaagagc gctggggcga tgccgggacc ggccgcttac ggccgcggcg ggacggagcc
     5701 gacgttcacg gatgccgccc tcgccatcgg ctacctcggc accgacaccc cgctcggcgg
     5761 tgagctcacg ctcgaccggg agaagtcgct cgagtcgctg gccccgatcg ccgggaccct
     5821 cgggctcagc gtcacccagc tggcgcgggg gatggtgcag atctcgaaca ccaagatcat
     5881 gggcgccgtc cgcgcgatca ccatcgagct cgggcacgac ccgaaggact tcgcactgct
     5941 ctcgttcggc ggcggcggag gcctcgtcgc ggtcgacgtg gcccgcgagc tcgggatccc
     6001 cgaggtgatc gtgccgccgg ggcagggggc gttctcggcg ctcgggatgc tgatggccga
     6061 cgtgcagcac gactactccc gcaccagcct gcaggcgctc gaggacctcg acgcggaggc
     6121 ggccgacgcc ctgtactccg gcatggaggc ggaggcgagg gagaccctgc gcgccgaggg
     6181 cttcgacgac gaccggatga tcttccaccg ggtggtcgcc gtgcggtact cgggccagga
     6241 gcactcggtc acggtcgact accccgagga cggcagcgat ccccgcgcgg ccatccgctc
     6301 ggagttcgac cggctgcatc tgcgtcagta cgggcacacc atggacgatc ccatcgagac
     6361 gacgactctt cgcctcggag cggtcggtgt ggtcgacaag cccgatctgc cgaaggcggc
     6421 gccccgcccc gacggaggag cgcccgcgct gcggacgcgc acggtcgtgc tcgacggcga
     6481 cgagacggcg gagtacgcgc tcgtctcgcg cgaggagctc atggccggcg acgtcctcga
     6541 aggtcccgcc gtcgtgaccg agcacacggc gacgaccgtg ctgcacgccg gtgaccgact
     6601 cgaggtcggc gcatacggcg aactcatcat ctcgatccag tacgcgaaga agggagcctg
     6661 acgtggccga cgccatcacc accgagatca tcaggcacgg cctgctggcc gccgcagagg
     6721 aggtcgcgcg caacctgtgc cgcaccgcct acaacaccgt ggtctacgag atccacgact
     6781 acggcatcgg catccacgac gccaacggcg acgtggtcgc cgacgcaccg gggatcgccg
     6841 tgttcaccag gggcaacgac gccggcatca agcactcgat ggagttcctg ggcgccgaca
     6901 ccatggagcc cggcgacgtc ttcatcatca actaccccta ctggtcgagc gcccacaccc
     6961 tcgaccccct cgtcttcgcc ccgatccacg tggacgacga gctgatcggc ttcgcctcat
     7021 gccgcgtgca cgtgaccgac ctgaaccaga aggaccccgg ttacgtgctc gactcgacgg
     7081 accgttcgca ggaggggctg atgctgcccg cgtcgaagct gtaccgcagg ggggagcgca
     7141 acgaggacgt gttcaacatc atccggttca acagccgcat gccgaagcac acgatcggcg
     7201 acatccaggc gcaggtctcg gcgtgcgtga ccggggtgcg ccgcacccag gagctcgccg
     7261 acaagtacgg cgtcggcacg ctgaccgagg cgatggtgac catcaacgag cacggtgaga
     7321 agctcgccag gctcgcgctc gcgaagctgc cgaagggcac gtggagcgcg gtggactacg
     7381 tcgacgacga cgggatcaac aaggacgagc tcgtcaggct gcagtgcacc gtgacggtgt
     7441 cggacgagga gatggtcgtc gactggaccg gcagcgccga gaacgtcgag ggtcccatca
     7501 acctgccgcc ggggcagacc gaggcgctct ccagcctgat cttcaagtcg ctcacgacgc
     7561 cggactcccc ggtcgtcgcg ggcaacttcg cgcccttgcg cgtggtcacg gtgcccggct
     7621 cggtcatgca cgccgtgccg ccgatgccca cgttcacgct ctggaccggc ctcctcgccg
     7681 gcgaggtgat cctcaaagcc ctggcccagg ggatgccaga gctcgtgccc gcctgctcgg
     7741 gcggcgacgt gtgctccatg atgggcctcg gcgtgaaccc gcgcaccggc gagccgtggc
     7801 aggaggcgac caacgaagcc gtcggtttcg gcggtaccgc gcgccacgac ggcgaggacg
     7861 ggatcatgca cctctcggag cccggatgcc gcaacaaccc ggtcgaggtt ctcgaggcga
     7921 aatcctcgat gatcatcgag cactacggct atcgcgccga caccggcggc gcgggcgagc
     7981 accgcggcgg ggtcggcgtg agtcggtcgt accgcttcac ggcacccgcc accggcatct
     8041 gcctcgtcta caagacgaag acggagccgt gggcgatcga cggcggtgcg gtcgggaagc
     8101 cgaatcgggt cgtgctcaac cccggcaccg accgcgaagt cgtgcagggc gggagctaca
     8161 accggctcga agcgggcgac gtgctcgtca accagacggg tgggggaggg ggctacggcg
     8221 acccgttgcg ccgtgatccc gccgccgtcg cgcgcgacgt gcgaaacggg ttcgtgtcgc
     8281 accgctcggc ggaacgcgac tacggcgtgg tggtgacggc cgacttcggc gtcgacgaga
     8341 cggcgacggc ggcgatccgg ggtcggtgag tcttcgtggt ggtgcgtccg ggacctccgg
     8401 atgcaccacc accgcatccg agcgcacatg ccgcctgctg catatcgacc cgcgtagcat
     8461 gaggacagcg atcggcatcc gcccttggct tccaaagctc aagggctccg atcgacagga
     8521 gagcatcatc tcactcactc aggtcgacac gaccaagacc accgttcccg cccagggacg
     8581 catccgacag acgtactccg gcactacgga gttcccgccg atggcgcgcg cctacaagga
     8641 tgtgctgaac gtcatccgcg atctgggcct gctgcgccgc acgccggtgt tctacacgct
     8701 ggtgggggtc gccctgctgc tcggcttcgc gggatgcgca gccggcttca tcctgctggg
     8761 agacagctgg ttccaacttc tcatcgcggg cgcgctcggc atcctgttca cacaggtcgc
     8821 cttcctcgca catgaggccg cgcaccgtca gatcctctcg tcgggcccgg cgaacttccg
     8881 tctcgcacgc gtgctcgcgg ggctcatcgg catgagctac tcctggtggg actccaagca
     8941 caccaagcat cacggcaacc cgaacatggt cggcaaggac cctgacatcg aggtcgacac
     9001 gatctccttc ctcgatgagg acgcggcccg ctcccgcggg gtcatccgct ggatcacgca
     9061 gcgtcagggg tggctgttct tcccgctgct cacgctggaa ggactgaacc tgcatcgcct
     9121 gagcgtggtg catctggtca cgcccggtcg cgtgaagggg cgctggaccg agatcggcat
     9181 catctccctg cggttcgctc tgctgctgac gccgctgttc ctgttccttc ccgtggggat
     9241 ggcgttcgcg ttcctgggcg tgcagatggc cgtgttcggc gtctacatgg gagcctcctt
     9301 cgccccgaac cacaagggca tgccgatcat ctccccggac gcgcggctcg acttcttctc
     9361 gaagcaggtc cgcacctcgc gcaacgtgac cggcggctgg tgggcgacct ggctgttcgg
     9421 tggcctgaac taccaggtcg aacatcacct cttccccagc atgtcgcgtc tgaacctctc
     9481 ccgcgcccgc gaggtggtgc gagactactg cgcggcgaat gacgttccgt acaccgagac
     9541 gaccgtgctg cagtcctacg gcatcgtcgt ccgctacctg aaccgggtcg gcctcgccgc
     9601 acgcgatccc ttcgactgcc cgatcaccgc ccagtaccgt ccggtgtgat cccgctcctt
     9661 gcagatctcg gccccgcagt caatgcggga gccgaacgcg cgacgccgtc ctagagtctc
     9721 cagacgtgcg gcacccgagc cgcaggattc aggagtgatc gatggccgcc atcgacgatc
     9781 aggcgacgca gtcgctgctc gacggtcgat atcgcctcga agcgtgcgtg ggtcgagggg
     9841 cgacgtccat ggtgtaccgc gccgaggatc tgctgctggg cagaacggtg gcgatcaagc
     9901 tgctgcgcca gtccgatgag acgcccatct cgctggagcg ggtccgcacc gaggtggcgc
     9961 tgctggcgaa tctgaagcac ccctccctcg tcaccctcta cgacgcgcga ctcgatccgg
    10021 gacagccgca ctacctggcg atggagtacg tcgagggtcc cacactcgcc tcgcgattgg
    10081 tcagcgggcc gctgtccgga gcagaggcgg cgtcggtcgc ccacgatctc gcagcgggac
    10141 tggatgccgt gcatcgcgcc ggcatcatcc acagggacgt caagccctcg aacgtgctga
    10201 tgcccgaagc gacccgcccc gccgatcacg catccgcgaa gctgacggac ttcggcatcg
    10261 ccttcgacgt ggatgatccg aggcgcacat cgccgggcat cgcgatcggc acggctgcct
    10321 acatggcgcc cgaacaggtg cgcgccgccg acctgacgac cgcggtcgac gtgtactcac
    10381 tcggtctcgt cctgatcgag gcgctcaccg gtgagccggc gttcccggtc gcgggtgggg
    10441 tgcagaccgc gctgcggcgg ctgaccgagc gtccggccat accggaatca ctggatgcag
    10501 gatggcgaac cctgctcgcg cgcatgacac gcgacgaccc ggcggagcga ccctcagccg
    10561 ccgaggtggc agcggaggcc gaggcgatcc gacgcacgga gacctccggt ggcggcatcg
    10621 gcaccgcgcc tccgatcccc acccgggcat cactccgcga tgtgaagacg aactccgatg
    10681 acgacgcgcc gaccgggcag acgagggcgt acaccgcagc cgcggtcgta ccgccacccc
    10741 gcttccgccg gacgcgcatc gccgtcgccg cggcgctggg tgcggcggcg ctgtcgctca
    10801 tcgccttcct cgggttccac ggcatcgcgc cctcggtcga atcgcccgcc cggatcgccg
    10861 tgcatgcgcc tcagacgtct ccgcccgcat cgcccgccgc ggatcctgct acgcagcagc
    10921 cgtcgacctc gagctccggc gcgagctcgg ggcagggcac gacgacgagt cagccgaatt
    10981 cgaacaaggg ccccggcaac aactccggga agggacaggg gagctcagga cacggcaagg
    11041 gccactgacg cgaccgagcg ccctgcctca gcggcggtgg gcccggtagt acgccaggag
    11101 tgccttggtc gacgcgtcct gcgcctccag ggcatccgcg tcgccctcga tcgcgggggc
    11161 gatctgcagg gcgagctgct tgccgagttc cacaccccac tggtcgaagg agttgatgcc
    11221 ccagatcacg ccctgcgtga aggtgatgtg ctcgtagagc gcgatcagct cgccgaggac
    11281 gcgcggcgtg agggcatcgg cgaagatcga cgtggtcggg cggttgccgg ggaatgtgcg
    11341 cgcggccacg agcgctccgg tggtgccctc ggcctcgacc tcctccgccg tcttgccgaa
    11401 cgccagcgcc ttcgtctgcg cgaggaagtt ggccaggaac aggccgtgca cgtcgcggcc
    11461 gtcgtcctgc agcggatagg cagggttcac gaaggcgatg aagtccgcag ggatcagccg
    11521 ggtgccctgg tggatcagct ggtagaacgc gtgctggccg ttggtgcccg gctcgcccca
    11581 gaagacctca ccggtggtcg tggtgacggg gctgccgtcc cagcgcaccg acttgccgtt
    11641 ggactccatg gtcagctgct gcaggtacgc ggcgaagcga ctcagctgct gcgcgtaggg
    11701 aagcacggcg tgcgactgcg cgccgaggaa gttcgtgtac cagacgttga gcagccccat
    11761 caggacgggc acgttctgcg cgagcggcgt ggtgcgcacg tgctcgtcga cggcgtggaa
    11821 gccggcgagc agatcgcgga acacctcggg tccgaacacg acggccagcg acagaccgat
    11881 cgcggagtcc accgagtagc ggccgcccac ccagtcccag aacccgaacg cgttggccgg
    11941 gtcgatgccg aaggcggcga ccttgtcgag tgccgtggag accgccacga agtggtgcgc
    12001 gacggcatcc gtcctgctct gctcgtcatc gccgatggca ccggatgccg acagccccgc
    12061 ccacagccaa tcgcgggcaa ggcgcgcgtt ggtgagcgtc tccagcgtgg tgaacgtctt
    12121 ggatgcgacg atgaacagcg tcgtctcggg gtcgaggtcc gcggtcttct gcgccaggtc
    12181 ggtggggtcg atgttcgaca cgaaacgggc ctggatgccc gcgtccgcgt acggcttcag
    12241 cgcctcgtac accatcaccg gaccgaggtc ggagccgccg atgccgatgc ccacgacgtg
    12301 cgtgaccttc ttgcccgtga cgcccttcca ctcgcccgag cgcacgcggt cggcgaaggc
    12361 ggagagcgcg tcgagcaccg actgcacatc ggcatccacg tcctgtccgt ccacgaccag
    12421 cgcgggcgtc gcccccgccg gacggcgcag ggcggtgtgg agcaccgcgc ggtcctcggt
    12481 ggtgttgatg tgctcgccgg cgagcatcgc ggcgtagcgc tcggcgacgc cggtctgctc
    12541 cgccaggcgc acgagggcgg cgaggatgtc gtcggtgacg aggttcttcg acaggtcgac
    12601 gtgcagatcg gccagcggca gcgagagtct ctcgacacgg cccgggtcgg cggcgaacca
    12661 gccgcgcaga tcgggggaga agcccgtgct gagggacgtg agctcggccc aggcggaggt
    12721 ggtggtcgca tcgatcggag cggtcatgca tgcaacgtta gcgccggatg cgggcatccg
    12781 cgcctccgtg tcgcaaccca gcccgcgcac atgagacgga catccccggg gactatcgtc
    12841 gcggcatgct cgaggcgatt ccgaaccggt cgggagcgat cgacctcgtc cgcatcatcg
    12901 cggtcgcggc gatcgtcgca gggcatgtct tcacccgcga tctgaccggc gacctgctct
    12961 acacctggca cgtcgcggtg ttcttcgtgc tctccgggta tctgtggaag cccggccgct
    13021 cgttccgcac cgagctgggc aggcgctggc ggtcgctcgg gcggccgtac ctggcggggt
    13081 tcgtcctgtt gacgatcgcg ctgctgtcca tggggttccc cgacaccctg cagcgcctcc
    13141 tcggcggtct gtacggcggc ggagtggcgg ggatgccgta catcacgctc tggttcatct
    13201 ccacgctgtt cttcaccgcg ctcctgctgc gcctgatcga acgcctgccc ctcgctctgc
    13261 agtggacgct gggcatcgcc ggggccgtcg ccggctggat ctggggatcc gcgctctcgg
    13321 acaccccgct cggcgtgggc atggcggcga cgagcctgat ctatctgctg gtcggcgacg
    13381 ccctgcgccg cgtgcgcgac cgggtgcgcg cgcc
//
LOCUS       DATMSX010000087         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_57976,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000087 DATMSX010000000
VERSION     DATMSX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57976"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VN041_02710"
     CDS             complement(<1..1126)
                     /locus_tag="VN041_02710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWU27987.1"
                     /translation="MTRAPSPPPELQGFEYLRPLGTGGFADVFLYEQQLPKREVAVKV
                     LLADRLSAGAAEEFTNEANVMAMLSTHPAIVTIYQAGVAADGRPYIVMEYCPKPNLQV
                     RTRKEPFSVAEALRVGVQVSGAVETAHRAGVLHRDIKPANILVTVYNRPALTDFGIAS
                     TTAADSEAAGMSIPWSPPESFAENPTTGVRTDVYALGATVFTLLSGRSPFERKGERNS
                     SADLIERIERMAVPKVDRADVPDSLQAVLARAMAKRPEDRYPSAVAFARALQKVQIEL
                     AHSVTPIDIVDEHPSHEELEDDDDGLTRVREVRSINPDTQSATRPSATTVRKQPSTPD
                     VPRFDRPSADALEETQMRTQRRGAAAPATADADDDDATVLR"
     gene            complement(1123..>1561)
                     /locus_tag="VN041_02715"
     CDS             complement(1123..>1561)
                     /locus_tag="VN041_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWU27988.1"
                     /translation="AEARRLRAEAATSAPDAPTEAIPVFENAPVAASGARIRLSTGQV
                     VDLDRTVIIGRKPRSTRTSGSTMPHLIAVDSPESDISRNHLEVRPEGDSVVVIDLHTT
                     NGSTLLRPGADPVRLHPGEQTLVLHGDVIDVGDGVTVTVEEQR"
BASE COUNT          204 a          530 c          585 g          242 t
ORIGIN      
        1 cgcgcagcac cgtggcgtcg tcatcatcgg cgtcggcggt cgcgggcgcg gcggctccgc
       61 gacgctgcgt gcgcatctgc gtctcttcca gcgcatccgc cgacggacgg tcgaatcgcg
      121 gcacgtcggg cgtgctcggc tgctttcgca cggtggtcgc cgagggacgc gtggccgact
      181 gcgtgtccgg gttgatcgac cggacctcgc ggacgcgcgt cagaccgtcg tcgtcgtcct
      241 ccagctcctc gtgcgaaggg tgctcgtcga cgatgtcgat cggggtaacg gagtgcgcga
      301 gctcgatctg caccttctgc agcgcacggg cgaacgccac cgcgctcggg tagcggtcct
      361 cgggtcgctt ggccatcgcc cgcgccagca ccgcctgcag gctgtcgggc acgtcggcgc
      421 gatccacctt cggaacggcc atgcgctcga tgcgctcgat cagatcggcg ctggagttgc
      481 gctccccctt gcgctcgaac ggcgagcggc cggagagcag cgtgaacacc gtcgccccca
      541 gcgcgtacac gtcggtgcgc acgccggtgg tcgggttctc ggcgaaggac tccggcggcg
      601 accacgggat cgacatgccc gcggcctcgc tgtcggcggc ggtggtcgac gcgatgccga
      661 agtcggtgag cgccggccgg ttgtagaccg tgacgaggat gttcgcgggc ttgatgtcgc
      721 gatgcagcac gcccgcgcgg tgggcggtct ccacggcgcc gctgacctgc acgccgacgc
      781 gcagcgcctc ggccaccgag aacggctcct tgcgggtgcg cacctgcagg ttcggcttgg
      841 ggcagtactc catcacgatg tacgggcgac cgtcggcggc gacgccggcc tggtagatcg
      901 tgacgatggc gggatgcgtc gacagcatcg ccatcacgtt cgcctcgttg gtgaactcct
      961 ccgccgcgcc ggcgctcagc cgatcggcca gcagcacctt gaccgcgacc tcgcgcttgg
     1021 gcagctgctg ctcgtagagg aacacgtcgg cgaacccgcc ggtcccgagc gggcggaggt
     1081 attcgaaccc ctgcagctcg ggaggcggag agggagcgcg cgtcatcgct gctcctcgac
     1141 ggtgacggtc acgccgtcgc ccacatcgat gacgtcgccg tgcagcacca gggtctgctc
     1201 accagggtgc aggcgcaccg ggtcggcgcc gggacgcagc agggtggagc cgttggtggt
     1261 gtgcaggtcg atgaccacga ccgagtcgcc ctcgggacgg acctcgaggt ggttgcgcga
     1321 gatgtcggac tcggggctgt ccaccgcgat caggtgcggc atggtgctcc cgctggtgcg
     1381 ggtggagcgc ggcttgcgcc cgatgatcac cgtgcggtcg agatccacga cctgaccggt
     1441 ggagagacgg atgcgagcgc cggatgccgc gacgggcgcg ttctcgaaga ccgggatggc
     1501 ctcggtcggc gcgtcgggcg cggaggtcgc ggcctcggcg cgcaggcgcc tggcctccgc
     1561 c
//
LOCUS       DATMSX010000088         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_76903,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000088 DATMSX010000000
VERSION     DATMSX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76903"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VN041_02720"
     CDS             complement(<1..715)
                     /locus_tag="VN041_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27989.1"
                     /translation="MSDSTPRSEVQQMSSKLMRSAIWIAIGSLIAAALVCVVWVLIGD
                     QDGIIGRAFLTILLLAVFAGIAIMESHLAEHRPEWLALSSMITWIVALLVGAVKIWMP
                     EHDPFGGWSLLRFWQLLLVVGIMQLALVHVRLFLPASKRYVTTFTRVIVIVTIALLAL
                     LVALLVFYLTFPDLFRYDDLYWRIVIALAILVAVGTTLIPLLNALFAPKKKPSFAPHA
                     APVGPPQGAAVAPQTAQGVT"
     gene            complement(804..1610)
                     /locus_tag="VN041_02725"
     CDS             complement(804..1610)
                     /locus_tag="VN041_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWU27990.1"
                     /translation="MTDPILFTVGQGLARLTLNRPASLNAFDADLAHAWRDVTAEAVA
                     RDDVHAILLDANGRAFCAGGDVLAMASGMGSGEDITALAHVINEGILSLTTSSIPVVA
                     AAHGTTAGGGLGVLMCSDYAVIGAGSKIGSLYANIGLTPDLSVTAQLARAIGERRALQ
                     LVLQDRMLSADEAVEWGLVAEAVAGAEGADAEAEAEAVRERAEQVARFWLKGASGAYG
                     QAKRLIRSQPARTFAEQVAEEARSIGEAFDTPDAKVRVAAFAAASSKSAR"
     gene            complement(1676..2167)
                     /locus_tag="VN041_02730"
     CDS             complement(1676..2167)
                     /locus_tag="VN041_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FBP domain-containing protein"
                     /protein_id="HWU27991.1"
                     /translation="MRPLTEDQIRASIRNADADEIAQIGMPHDFILTDWDYLDFLAWR
                     DPESSRRAVVIVEHEGQPMGIVLRATDPARVRSGMCNICHTLQPGNQVALLTARRGGA
                     KGRRGDSAGTYICADLSCHDNVRLAHPLAPNEVRAPGQADMRLDGTRHRMEAFVVRVL
                     SED"
     gene            complement(2272..2973)
                     /locus_tag="VN041_02735"
     CDS             complement(2272..2973)
                     /locus_tag="VN041_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWU27992.1"
                     /translation="MTTNRTIRTGVFALITALAVGVLTACSPASGRSGNSGTDATKAL
                     WSAVDSGDADAARQAIDDGADLESRGPNDATPVIAATKKNDARVARVLIDAGADVNAK
                     DGIQDSAFLYAGAEGLDEILKMTIDAGADVKSTNRYGGTALIPASEHGHVSTIRILIA
                     AGVPVDHVNDLGWTAMHEAIVLNDGGPDQVKAVRLLLEAGADPSITENGGLTARELAE
                     QKGYDEIVAVIDAAA"
     gene            3378..4571
                     /locus_tag="VN041_02740"
     CDS             3378..4571
                     /locus_tag="VN041_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27993.1"
                     /translation="MLRSTDGSVLVRFIDGWWDGHVTPPPLGRVLRWMLRVTPFMTLM
                     VIAGWMLDLQAAAQTAPRRWKYPWEVLGIAAAAFALLFLPALMILLVIANLVIIRRPD
                     AWPRLTDIVLSTMGDAWLYRSDELDDDVLSRLRAQLNDVRDSSDHVVLVGHSQGAEIG
                     RRLALDGGIDACIWVGGALIPLAVARTMRRSRSLPYVLWCYVLISPLLFSLLVASLLQ
                     TLGGFIWTLLDSFLSVVSGAVTPTAGLEPMRQAPWDLTGSFLAHLPLLLGFATFSLLS
                     ALLLRKLARWPSDLTQSPDCPVIMVKSLADPVCFGHLDTAAAVRYVPIRAGRAVWREH
                     VAYFEKPETGSALWEPLLGPEDAPPPYEPRFGVMTKIVATVAAAGLIAAAYALGSAAL
                     NAFGW"
     gene            complement(4627..4710)
                     /locus_tag="VN041_02745"
     tRNA            complement(4627..4710)
                     /locus_tag="VN041_02745"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4674..4676),aa:Tyr,seq:gta)
     gene            complement(4841..>5149)
                     /locus_tag="VN041_02750"
     CDS             complement(4841..>5149)
                     /locus_tag="VN041_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWU27994.1"
                     /translation="LGVRAVLATAGADGAVLETEAGKLTAPIAALPGRVVDTVGAGDA
                     TLAGVAEGLVDGHPSSAAEWQALLDRAMLIAAATVRSEGGLLRTPESLAESERGVFGS
                     "
BASE COUNT          794 a         1767 c         1741 g          847 t
ORIGIN      
        1 cggtgacgcc ctgggccgtc tgcggagcga ccgccgcgcc ctgcggcgga cccaccggag
       61 ccgcgtgcgg cgcgaacgag ggcttcttct tcggagcgaa cagggcgttc agcagcggaa
      121 taagcgtcgt tcccacagcc accaggatcg cgagcgcgat cacgatgcgc cagtacaggt
      181 cgtcgtagcg gaagaggtcg gggaaggtca ggtagaagac cagcagcgcc accagcagcg
      241 cgagcagcgc gatggtgacg atcacgatca cgcgcgtgaa ggtggtgacg tagcgcttcg
      301 acgccggcag gaacaggcgc acgtgcacca gcgcgagctg catgatcccg accacgagga
      361 gcagctgcca gaagcgcagc agcgaccatc cgccgaacgg gtcgtgctcc ggcatccaga
      421 tcttcaccgc gcccaccagc agcgcgacga tccacgtgat catgctcgag agggccagcc
      481 actccggccg gtgctccgcg agatgcgact ccatgatcgc gatgccggcg aacaccgcga
      541 gcagcaggat cgtcaggaac gcccggccga tgatcccgtc ctggtcgccg atcagcaccc
      601 agacgacgca gaccagggcc gctgcgatca gcgatccgat cgcgatccag atggccgagc
      661 gcatgagctt cgacgacatc tgctggactt cggaacgtgg cgtgctgtcg gacatgctga
      721 tcctcccggc gatggtcatc ccatcctgcc atgcggcgcc atgacggccc gccggctgtg
      781 gataacccgg ccggggcagg tcgtcagcgc gcggacttgc ttgatgccgc ggcgaacgcg
      841 gcgacacgca ccttggcatc aggcgtgtcg aacgcctcgc cgatggaacg cgcctcctcc
      901 gcgacctgct cggcgaacgt gcgcgcgggc tgcgagcgga tcaggcgctt ggcctgcccg
      961 tacgcgccgg aggcgccctt caaccagaag cgtgcgacct gctcggcgcg ctcgcgcacg
     1021 gcttccgctt ccgcctcggc atccgcacct tcggcgccgg cgacggcctc agcgaccaga
     1081 ccccattcca cggcctcgtc ggcggacagc atccggtcct gcagcaccaa ctgcagtgcg
     1141 cggcgctcgc cgatcgcccg cgccagctgc gcggtgaccg acaggtcggg cgtgagtccg
     1201 atgttggcgt acaggctgcc gatcttggac cccgccccga tgacggcgta gtcactgcac
     1261 atcaggactc cgaggccgcc gccggcggtc gtgccgtgcg ctgccgcgac caccgggatc
     1321 gacgaggtgg tcagcgaaag gatgccctcg ttgatgacgt gcgcgagcgc ggtgatgtcc
     1381 tcaccgctgc ccatgccgct cgccatcgcg agcacgtcgc cgccggcgca gaaggcacgg
     1441 ccgttggcat ccagcaggat cgcgtgcaca tcgtcgcgtg cgacggcctc ggcggtgacg
     1501 tcgcgccagg cgtgggcgag gtcggcgtcg aacgcgttca ggctggcggg gcggttcagc
     1561 gtcagccggg caagcccctg gccgaccgtg aacaggatgg gatcggtcat ggctctcctt
     1621 cgacgctccg acacgcagat cttgagtctc agcctagctg ggactgaggt tcatgtcagt
     1681 cctcggacag gacccgcacg acgaacgcct ccatgcggtg acgcgtgccg tccagtcgca
     1741 tgtcggcctg tccgggcgcg cgcacctcgt tcggagccag cggatgcgcc agccgcacgt
     1801 tgtcatggca cgagaggtcc gcgcagatat aggtgcccgc gctgtcgccg cggcggccct
     1861 tcgctccccc acggcgcgca gtgagcagtg ccacctggtt gcccggctgg agcgtatggc
     1921 agatgttgca catgcccgac cgcacccgtg cggggtcggt ggcgcgcagc acgatcccca
     1981 tcggttggcc ctcgtgctcc acgatcacga ctgcacggcg gctggactcc gggtcgcgcc
     2041 aggcgaggaa gtcgagataa tcccagtcgg tcaggatgaa gtcgtgcggc atcccgatct
     2101 gggcgatctc atcggcatct gcgttgcgga tcgacgcgcg gatctggtcc tcggtgagtg
     2161 gtcgcatcga ttcagtctaa gaaggatgtg ccacgaaggg gtgtcgcgcg gccggggacg
     2221 caacagccgg ccgcgcgagt ctgtggggcg atcagcaagg acccgactcg ctcacgccgc
     2281 cgcgtcgatc acggccacga tctcgtcgta gcccttctgc tcggcgagct cgcgcgcggt
     2341 gagtccgccg ttctcggtga tgctcgggtc cgctcccgcc tcgagcagca ggcgcacggc
     2401 cttgacctga tccgggccgc cgtcgttcag gacgatcgcc tcgtgcatcg cggtccagcc
     2461 caggtcgttc acatggtcga ccggcacgcc ggcggcgatc aggatgcgga tcgtgctgac
     2521 gtgcccgtgc tcgctcgccg ggatgagcgc tgtgccgccg tagcggttgg tgctcttcac
     2581 gtcggcgccc gcgtcgatgg tcatcttcag gatctcgtcg aggccctcag cgccggcgta
     2641 gaggaatgcg gagtcctgga tgccgtcctt cgcgttcacg tcggcgcccg cgtcgatgag
     2701 cacccgggcg actcgtgcat cgttcttctt ggtcgccgcg atcaccgggg tcgcgtcgtt
     2761 cgggccgcgc gactcgagat cggcgccgtc gtcgatggcc tgccgggcgg cgtcggcatc
     2821 cccggagtcg accgcgctcc acagcgcctt ggtcgcgtcg gttccgctgt tgccggacct
     2881 accggacgcc ggagagcacg ccgtcaggac gccgacggcg agcgcggtga tgagcgcgaa
     2941 cacgcccgtg cggatggtgc gattcgtggt catgcacgcc tcctcaggtc aggatcgaaa
     3001 tggtatacca aaactcctga gggtggcctg ggagcgtctg cccgcagccc catagggagc
     3061 agcatggctc gagtcagcga cgatcaccac aggagcatcc gaatgatcgc gcgtggtgat
     3121 tctctgatgt cgtaggcccg ctctaccgtg gcggcatgag agagcaggaa tccacggagc
     3181 cagcggacgc gtctccacct cccggggcga cccccgaaac ggcgccggtg cggattgcct
     3241 acttcgcgat ccatggcatc ggacgccaag gagagggtga gacggcaggc gacgtggcga
     3301 gtgccttcag cgatgccgcc gtcgcattgg gcatagacct cgaagaagcc ggatcgcccg
     3361 catccgagga ctcctcagtg ctgcggtcca cagacggcag tgtcctcgtt cgattcatcg
     3421 acggctggtg ggacggacac gtcacgcccc caccgctcgg ccgcgtgctg cgctggatgc
     3481 tgcgagtgac accgttcatg acgctgatgg tgatcgcggg atggatgctg gatctccagg
     3541 ctgcggctca gaccgcgccg cggcggtgga agtatccgtg ggaggtcctg ggcatcgcgg
     3601 cggcggcctt cgctctgctg ttcctgccgg ccctgatgat cctcctggtc atagcgaacc
     3661 tcgtgatcat caggaggccc gatgcctggc cgagactgac cgacatcgtc ctctcgacga
     3721 tgggagacgc gtggctgtac cgctccgacg aactcgacga cgacgttctc tcgcggctgc
     3781 gcgcgcaact caacgatgtg cgcgattctt ccgatcacgt ggtgctcgtc ggacattccc
     3841 agggtgcgga gatcggtcgc cggctggctc tggacggcgg gatcgacgcg tgcatctggg
     3901 tgggcggcgc gctgataccg ctggcggtcg cacgcacaat gcggcgcagc cggtctctgc
     3961 cctacgtgct gtggtgctac gtgctcatct cgccgctgct gttcagtctt ctcgtcgcct
     4021 ctctgctgca aacactcggc ggcttcatct ggacactgct ggattcgttc ctctccgtcg
     4081 tctccggcgc ggttacgccg accgcgggac tcgaacctat gcgtcaggcg ccgtgggacc
     4141 tgacgggatc ctttctcgcg cacctaccac tgctgctcgg tttcgccacg ttctcgctcc
     4201 tgtcggcact gctgctccgg aagctcgcgc ggtggccgtc cgacctcacg cagtcgccag
     4261 actgtcctgt catcatggtc aagtcgctcg ctgacccggt gtgcttcggg cacctggaca
     4321 ccgcggcggc tgtgcgctac gtgccgatcc gcgcgggacg cgcggtgtgg cgcgagcacg
     4381 tcgcctattt cgagaagcca gagaccgggt cagccctgtg ggagccgctg ctcgggcccg
     4441 aggatgctcc tccgccgtac gaaccgagat tcggcgtgat gacgaagatc gtcgcaaccg
     4501 tcgcagctgc cggcctgata gcggcagcct atgccctagg ttctgccgcg ttgaacgcct
     4561 tcggatggtg agtgatccgg acgagaaaga ctgaaggccc ccgctacgcg agggccttca
     4621 gtcacatggt ggcaagtgag ggattcgaac ccccgaagct tacgcggctg atttacagtc
     4681 agctcccttt ggccgctcgg gcaacttgcc agtgcacccc cgtccggctt gtccagtcaa
     4741 ccgggggcgc gaagatcaag catacctgcc ggagtcgccc ggtaagaaat cgaactccga
     4801 tcccgcgcgc gccgctgctc gtgggggcca cgtcccggcg tcagctgccg aagacgccgc
     4861 gctccgactc ggccagcgac tcgggcgtgc gcagcaggcc tccctcggag cgcacggtag
     4921 cggcggcgat cagcatcgcc cgatcgagca gcgcctgcca ctccgcggcc gacgacggat
     4981 gtccgtcgac gagtccctcg gcgacccccg ccagcgtggc atcacccgcg ccgacggtgt
     5041 cgacgacacg accgggcagc gcggcgatcg gggcggtgag cttaccggcc tccgtctcca
     5101 ggaccgctcc gtcggctccc gccgtggcca gcaccgcgcg cacgcccag
//
LOCUS       DATMSX010000089         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124420,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000089 DATMSX010000000
VERSION     DATMSX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124420"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN041_02755"
     CDS             complement(<1..282)
                     /locus_tag="VN041_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU27995.1"
                     /translation="MPESRTTRTYRSELRTEQAATTRRRVLDAAAASFTEGGYSGVSL
                     AEIGRRAGVSAETVKANGPKRDLLLGAFEQAIAGAEGDAQLADSTEGVDR"
     gene            367..>1553
                     /locus_tag="VN041_02760"
     CDS             367..>1553
                     /locus_tag="VN041_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HWU27996.1"
                     /translation="MTSILLAATPVHGHVAPLLAVASSLVAAGDRVRFLTGSRYRDAV
                     LSTGADFLPLPAEADYDDRDMDAAFPGRRGLKGPAGIRYDMIEIFLKPVPAQLRAVRE
                     AIAAERTDAVLAESMFAAAAVLSGLPRDERPLLVNLGIVPLALKHPDVAPFGLGIRPH
                     PGPMGRLRNAVLTTMAEKGVFAPVQRFADAIARAETGRGLTRFFLDWPAGADLLVQFT
                     VPEFEYPRPGMPDTVHFVGPVSRARVSTASLPEWWSDLEGRRVVHVTQGTVANSDWAL
                     VTAATAGLADADAIVVVSTGGRPVDSIPGPLPDNVRVASFLPYDRLLPLTDVLVTNGG
                     YGGVHYALESGVPIVVAGRTEDKIEVSARIAWSGVGIDLRTEAPTPAQVGAAVRAVLD
                     DPS"
BASE COUNT          204 a          567 c          532 g          250 t
ORIGIN      
        1 ccgatctacg ccctccgtgc tgtcggcgag ctgcgcgtcg ccctccgcgc cggcgatggc
       61 ctgctcgaag gcgccgagca gaagatcgcg cttcgggccg ttggccttga cggtctcggc
      121 ggagacgccc gctcgccgtc cgatctcggc gagcgagacg ccggagtagc cgccctccgt
      181 gaagctcgcg gcggcggcgt cgagcacgcg gcggcgggtg gtggcggcct gttcggtgcg
      241 cagttccgaa cggtaggtgc gggtcgtgcg ggattcgggc atcgttcttc ctattgacaa
      301 ccatgatgag gtagtgaatg cttcatcggt gtagtcgatc atatcccctg acccgaggcc
      361 ttcaccatga cgtccatcct tctcgccgcc acacccgtcc acggacacgt cgcacctctg
      421 ctggcggtcg cctcgtcgct cgtcgccgcg ggcgatcgcg tcaggttcct caccggctcc
      481 cgatatcgcg acgccgtgct ctccaccggc gcggacttcc tgccgctgcc cgccgaggcc
      541 gactacgacg accgcgacat ggatgccgcc ttccccggcc gccgaggcct gaagggcccc
      601 gcgggcatcc gctacgacat gatcgagatc ttcctgaagc ccgtccccgc gcaattgcgc
      661 gcagtgcgcg aggccatcgc cgccgagcgg accgacgccg tcctcgcgga gtcgatgttc
      721 gccgcagcgg ccgtgctgag cggcctgccc cgcgatgagc gtccgctgct cgtcaacctc
      781 ggtatcgtgc cgctcgccct gaagcatcct gatgtcgccc ccttcggcct cggcatccgc
      841 ccccatcccg gcccgatggg gcgtctgcgc aacgccgtgc tgacgaccat ggctgagaag
      901 ggcgtgttcg cgcccgtgca gcgcttcgcc gacgcgatcg cacgggccga gaccgggcgc
      961 gggctgacgc ggttcttcct ggactggccg gccggcgccg atctgctcgt gcagttcacc
     1021 gtgccggagt tcgagtaccc gcgtcccgga atgcccgaca ccgtgcactt cgtggggccg
     1081 gtgtcacgtg cgcgcgtctc gaccgcctcg ctgcccgagt ggtggagcga tctcgagggg
     1141 cggcgggtgg tgcacgtgac gcagggcacc gtcgccaact ccgactgggc actggtgacg
     1201 gcggccacag ccgggctcgc cgacgccgac gcgatcgtgg tcgtgagcac cggaggaagg
     1261 cccgtggact ccatccccgg tccgcttccg gacaacgtgc gagtcgcgag cttcctcccg
     1321 tacgaccggc tgctgccgct caccgacgtg ctcgtcacca acgggggtta cggaggagtg
     1381 cactacgccc tcgagagcgg ggtgccgatc gtcgtcgccg ggcgcaccga ggacaagatc
     1441 gaggtgagcg cccgcatcgc ctggagcggc gtggggatcg acctgcgcac cgaagcgccg
     1501 actccggcgc aggtcggcgc cgcagtccgc gcggtgctgg acgacccgtc cta
//
LOCUS       DATMSX010000090         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_58059,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000090 DATMSX010000000
VERSION     DATMSX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58059"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1756
                     /locus_tag="VN041_02765"
     CDS             116..1756
                     /locus_tag="VN041_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HWU27997.1"
                     /translation="MSDGPLIVQSDRTVLLEVAHADAETARHELAIFAELERAPEHIH
                     TYRITRLGLWNARAAGHTADDMLETLDRWSRFPVPPSVSVDLRETVDRYGRLVIERDE
                     EGALILRSDDAAVLTQVAGNKRIQPLLIGHPTSQSYTIDAWARGQIKQELLKIGWPAE
                     DLAGYTPGTPHEIELAEDGWAMRPYQRQAVDAFAHEGSGVVVLPCGAGKTIVGAGAMA
                     ATGTTTLILVTNTVSARQWRDELLKRTSLTPDEIGEYSGQAKEVKPVTIATYQILTAK
                     RKGQYAHLALLDALDWGLIVYDEVHLLPAPVFKLTADLQARRRIGLTATLVREDGREG
                     DVFSLIGPKRFDAPWKQIEAQGFISPAACYEVRVDLPPSDRLEYAAATDDERYRLAAS
                     APAKITAVRELIAKHPDERILVIGQYLDQLEELSEALNAPQITGATPVDEREELYRQF
                     REGEISLLVVSKVANFSVDLPEASVAIQVSGSFGSRQEEAQRLGRLLRPKQSNHTASF
                     YTLIARDTVDQDYAQNRQRFLAEQGYSYTIMDAVDLAA"
     gene            complement(1758..1892)
                     /locus_tag="VN041_02770"
     CDS             complement(1758..1892)
                     /locus_tag="VN041_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU27998.1"
                     /translation="MSEFEGGDSACWLSKVCPECGALLDDLASPCWRCGLKPGEEPRD
                     "
     gene            complement(1903..>2836)
                     /locus_tag="VN041_02775"
     CDS             complement(1903..>2836)
                     /locus_tag="VN041_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWU27999.1"
                     /translation="AEELAFHRLDRARSRPPRWWRPLLVALVAVGLYLAMMVAIMVPL
                     VIIAMQVPAFASTFDALTRGEIPGFDVADPTGFLLLVVPLILMIPALLVASRMLGGRG
                     VGLLLSVTGRLRWGWLGRSLLVALAVFVVQTAVSLALAVATGESIAVGASQPGLALML
                     VLIVVLVPLQSAAEEYVFRGFLMQAIGTWLRHPLFPILLPIPLFVFGHLYDVWGLLSV
                     GVFAVTAAWLVWRTGGLESAIALHAVNNVSAFLLGAFGLADANATTGSPLDVLTAAIT
                     MVAYAGIIEWMQRRRGLVRTRTVQLRAPMPVPQG"
BASE COUNT          489 a         1030 c          962 g          355 t
ORIGIN      
        1 cgcgaccgcg ccgccgacgt cgagcggacg ctcccggtgc gcagcatccg ctcggtgcgg
       61 gtgctcgaga gctgagcaga gcccctcccg gacgtcgccg gtagactggt ctgctatgtc
      121 tgatggcccc ctgatcgtcc agagcgaccg caccgtgctg ctcgaagtcg cgcacgccga
      181 tgccgagacc gcacgtcacg agctggcgat cttcgcggag ctggagcgcg cccccgagca
      241 tatccacacc taccggatca cccggctggg gctgtggaac gcccgcgcgg ccggccacac
      301 ggccgacgac atgctggaga ccctggaccg ctggtcccgc ttccccgtgc cgccgtcggt
      361 ctcggtcgac ctgcgtgaga ccgtggaccg ctacggccgt ctcgtcatcg aacgggatga
      421 agagggcgcc ctgatcctgc gctcggacga cgcggccgtg ctcacccagg tggcgggcaa
      481 caagcgcatc cagccgctgc tgatcggcca cccgacctcg cagagctaca cgatcgacgc
      541 ctgggcgcgc ggtcagatca agcaggagct gctgaagatc ggctggcccg ccgaggatct
      601 cgcgggctac acccccggca ccccgcacga gatcgagctc gcggaggacg gctgggcgat
      661 gcgtccgtac cagcggcagg ccgtcgacgc gttcgcgcac gagggctccg gagtggtcgt
      721 cctcccctgc ggcgcgggca agacgatcgt cggcgcaggc gccatggccg cgaccggcac
      781 gacgacgctg atcctcgtga ccaacacggt ctccgcgcgg cagtggcgcg acgagctgct
      841 gaagcgcacc tcgctcaccc cggacgagat cggcgagtac tccggccagg ccaaggaggt
      901 caagccggtc accatcgcga cctaccagat cctcactgcc aagcggaagg gccagtacgc
      961 gcacctcgct ctgctggatg ccctggactg gggcctcatc gtctacgacg aggtgcacct
     1021 gctgccggcc ccggtgttca agctcaccgc cgacctgcag gcgcgtcgcc gcatcggcct
     1081 gaccgcgacc ctggtgcgcg aggacggccg cgagggcgac gtgttcagcc tgatcggccc
     1141 caagcgcttc gacgccccct ggaagcagat cgaggcgcag ggcttcatct cccccgccgc
     1201 ctgttacgag gtgcgagtgg acctgccgcc cagcgaccgg ctggagtacg ccgcggccac
     1261 cgacgacgag cggtaccggc tcgcggcatc cgccccggcg aagatcacgg ccgtgcgcga
     1321 gctgatcgcg aagcaccccg acgagcggat cctggtgatc gggcagtacc tcgatcagct
     1381 cgaggagctg tccgaggcgc tgaacgcccc gcagatcacc ggcgccacac ccgtggacga
     1441 gcgcgaggag ctgtaccggc agttccgcga gggtgagatc tcactgctgg tcgtgtcgaa
     1501 ggtcgcgaac ttctcggtgg acctgccgga ggcctcggtc gcgatccagg tctcgggctc
     1561 cttcggctcc cgccaggagg aggcgcagag gctcggacgc ctgctgcgtc ccaagcagtc
     1621 gaaccacacg gccagcttct acacgctgat cgcccgcgac acggtcgatc aggactatgc
     1681 gcagaaccgg cagcggttcc tcgcggagca ggggtacagc tacacgatca tggacgccgt
     1741 cgacctggcg gcctgagtca gtcgcgcggc tcctcccccg gcttcagccc gcatcgccag
     1801 cacggcgagg cgaggtcgtc gagcagcgca ccgcactccg ggcacacctt cgagagccag
     1861 caggcgctgt cgccgccctc gaactctgac atgtcccgga tgctacccct gcgggaccgg
     1921 catcggcgcg cgaagctgca cggtccgggt gcgcaccagc ccgcgccgcc gctgcatcca
     1981 ctcgatgatc cctgcgtacg cgaccatcgt gatcgccgcg gtgagcacgt cgagcggcga
     2041 tccggtcgtg gcgttcgcgt cggcgagccc gaaggcgccg agcaggaacg cggagacgtt
     2101 gttcacggcg tggagcgcga tcgccgactc caagccgccc gtgcgccaca ccagccaggc
     2161 ggccgtgacc gcgaacacgc cgacactgag gagtccccac acgtcgtaga ggtgcccgaa
     2221 cacgaacagc gggatcggca gcaggatcgg gaagagcgga tgccgcagcc aggtgccgat
     2281 cgcctgcatg aggaacccgc ggaacacgta ctcctcggct gcggactgca gcgggacgag
     2341 cacgacgatc agcacgagca tcagcgcgag ccccggctgc gaggcgccca ccgcgatgga
     2401 ctcgcctgtg gccacggcga gcgcgagcga caccgccgtc tgcacgacga acacggcgag
     2461 cgcgacgagc aggctgcgtc cgagccagcc ccagcggagg cggccggtga ccgacagcag
     2521 cagtcccacg ccccgtccgc cgagcatccg ggacgccacc agcaacgcgg ggatcatcag
     2581 gatcagcggg acgaccagga gcaggaaccc tgtgggatcg gcgacgtcga acccggggat
     2641 ctcaccacgg gtgagtgcgt cgaatgtact cgcgaacgca ggcacctgca tcgcgatgat
     2701 gaccagcggc accatgatcg cgaccatcat ggcgaggtac agacccacgg ccacgagcgc
     2761 caccagcagc ggccgccacc agcgcggcgg ccgggagcgc gcacgatcca gccggtggaa
     2821 ggcgagctcc tccgcc
//
LOCUS       DATMSX010000091         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39268,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000091 DATMSX010000000
VERSION     DATMSX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39268"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VN041_02780"
     CDS             complement(<1..1251)
                     /locus_tag="VN041_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HWU28000.1"
                     /translation="MRSGSREALVAFADRMLSGAAAWASPGFARITPPGAPGGYGTAV
                     DGLEGFARTFLLAGFRIAGARGEGVDQLIHDYSRGIAAGVDPASEERWVRLDEHPQAK
                     VEAASLALILDMTRPWIWDRLDASTQQRVIEYLSPVVGDTSYPQNNWVWFRIVVQTFL
                     RSVGGPWSASDIAADLARHDSFARGDGWFSDGDDRNYDHYVGWALHLYPVLWSRMQGA
                     AEIAPERFAGDVEALDGYLQDAVHLVGADSGPLIEGRSLIYRFAAAAPFWVGAFAQVP
                     STDLGTLRRTAMGIIAHFDEHGAPDGDDLLTMGWHGEWRRLAQSYSGPGSPYWASKGM
                     LGIALPADHPVWAAPEPAPFLDDLRVVAPAGWIVSGTASDGIVRVINHGTDHAQPGAV
                     AGDSPLYARLGYSTAASPLLNGDAW"
     gene            1462..>1941
                     /locus_tag="VN041_02785"
     CDS             1462..>1941
                     /locus_tag="VN041_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWU28001.1"
                     /translation="MRAVIMHGPGDVRVEELPLPSLVDPTDAIIRLDATCICGSDLWP
                     YRGIQPIKRPIRMGHEYIGTVEQVGAEVRDIAVGDFVVGSFMASDGTCEICQAGYQSR
                     CIHVVGMGSVGTQAEYARVPWADGTLVKVTGDLTPERRRSLLAASDVLGTGWFAAVAA
                     "
BASE COUNT          289 a          662 c          711 g          279 t
ORIGIN      
        1 ccacgcgtcg ccgttcagca gcggggacgc ggcggtggag tagcccagtc gcgcgtacag
       61 cggggagtcg cccgcgaccg cccccggctg cgcgtggtcg gtgccgtggt tgatcacgcg
      121 gacgatgccg tcggatgccg tgccggagac gatccagccg gcgggtgcca ccacgcgcag
      181 gtcgtcgagg aacggcgcgg gttccggggc cgcccagacc gggtggtcgg cgggaagcgc
      241 gatgcccagc atccccttcg acgcccagta gggcgacccg gggccggagt agctctgcgc
      301 gaggcgccgc cactcgccgt gccagcccat cgtgagcagg tcgtccccgt ccggagcgcc
      361 gtgctcgtcg aagtgcgcga tgatgcccat cgcggttcgg cgcagcgtgc cgaggtcggt
      421 ggacgggacc tgcgcgaacg cgccgaccca gaagggagcg gcggcggcga accggtagat
      481 caggctgcgc ccctcgatca gcgggccgct gtcggcgccg accaggtgca cggcatcctg
      541 caggtagccg tcgagtgcct cgacgtcgcc ggcgaagcgc tcgggggcga tctcggcggc
      601 accctgcatg cgtgaccaga gcaccgggta caggtgcagc gcccagccga cgtagtggtc
      661 gtagttgcgg tcgtcgccgt cggagaacca gccgtcgcca cgggcgaagg agtcgtggcg
      721 ggcgaggtcg gcggcgatgt cggatgccga ccagggaccg ccgaccgagc gcaggaaggt
      781 ctgcaccacg atgcggaacc acacccagtt gttctgcggg taactggtgt cgcccacgac
      841 gggcgagagg tactcgatca cgcgctgctg ggtcgacgcg tcgagccggt cccagatcca
      901 gggccgggtc atgtcgagga tgagtgcgag cgaggcggcc tcgaccttgg cctgcggatg
      961 ctcgtcgagc cgcacccagc gctcttcgga cgccgggtcg acgccggccg cgatcccgcg
     1021 gctgtagtcg tggatcagct ggtccacgcc ctcgccgcgc gcgccggcga tgcggaagcc
     1081 cgccagcagg aaggtgcggg cgaagccctc caggccgtcc accgccgtgc cgtagccgcc
     1141 gggggcaccg ggaggcgtga tgcgcgcgaa gcccggcgac gcccatgctg ccgcgccgct
     1201 cagcatccga tcggcgaagg cgaccagtgc ctccctcgat ccgctccgca gcgggctgcg
     1261 atgaggagtc gtcggcgtgg gggtcacagg gtctccttcg aacagcgatc cgcacccggg
     1321 aaagggtgac tgtttcagga gattagcaat ccgttttgat cgcctcaaga atgtttacgt
     1381 tccaccatga acgttcatga tcgtttatcg aaacgtgtcc cgcgcgcccg gggaagcgct
     1441 tgccgggcgc atagtggaag catgcgcgca gtcatcatgc acggtcccgg cgacgtccgg
     1501 gtcgaagagc tccccctgcc ctccctcgtc gacccgacgg atgccatcat ccggctcgac
     1561 gcgacctgca tctgcgggtc ggatctctgg ccctaccgcg gcatccagcc gatcaagcgc
     1621 cccatccgga tgggtcacga gtacatcggc acggtcgagc aggtcggcgc ggaggtgcgg
     1681 gacatcgcgg tcggcgactt cgtggtcggc tcgttcatgg catccgacgg cacctgcgag
     1741 atctgccagg ccggctacca gtcccggtgc atccacgtgg tgggcatggg ctcggtggga
     1801 acgcaggccg agtacgcacg cgtcccctgg gccgacggca cgctggtgaa ggtgaccggc
     1861 gacctgaccc ccgagcgccg gcgcagcctg ctcgccgcat ccgatgtgct cggcaccggg
     1921 tggttcgcgg cggtcgccgc c
//
LOCUS       DATMSX010000092         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_77009,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000092 DATMSX010000000
VERSION     DATMSX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77009"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VN041_02790"
     CDS             complement(<1..497)
                     /locus_tag="VN041_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU28002.1"
                     /translation="MTDRNDTLFDAARAVIPGGVNSPVRAYGSVGGTPRFLASANGAR
                     VTDAAGTEYIDLVASWGPALLGHTHPEVVRAVQEAATRGLSFGAPTEGEVELAQLIAD
                     RVQVGDARPVEHVRMVSTGTEATMTAIRLARGATGRDLLVKFAGHYHGHSDGRTGRQG
                     SPASRG"
     gene            complement(919..1896)
                     /gene="hemB"
                     /locus_tag="VN041_02795"
     CDS             complement(919..1896)
                     /gene="hemB"
                     /locus_tag="VN041_02795"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWU28003.1"
                     /translation="MTRFPETRLRRLRQSAPVRSLVRETSLSAQQLVLPMFVREDVSE
                     PAPIGSMPGVVQHSLDSLRRAAADAAETGIGGVMLFGIPEVRDADGSGADDPEGILNV
                     ATAAVAAEVGDALVVQTDLCLDEFTDHGHCGVLDTSGAVDNDATLDRYRSMALAQARA
                     GSQLLGLSGMMDGQVAAIREALDTEGFQDTLLLAYSAKYASAFYGPFREAVDSQLKGD
                     RRTYQMDAGNRREGVREALFDVDEGADIVMVKPAMAFLDMVREVRDAVDVPVWAYQVS
                     GEYAMIEAAAANGWIDRRAAILESLLSIRRAGADAVLTYWAVEAAEWLR"
     gene            complement(1893..2705)
                     /locus_tag="VN041_02800"
     CDS             complement(1893..2705)
                     /locus_tag="VN041_02800"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWU28004.1"
                     /translation="MTASETKTAKPLDGWRVLVPRGGPWGDSVAASLRAQGAVPVVAP
                     LINFAPTTDQDALDAALERLQEGHYDWLTVTSATTVDVLFAHRVTVPKRTKIAAVGET
                     TAAALLAVGYEVALVPELDNSAAGMAEQMIALEPEPRRVLSLRSEIAKPVLSVMLNDA
                     GHDVDSVVAYRTVGVPVTDRIRRDVENGRINAILITSGSVASQVREQFPEIPDSTLLA
                     AIGPRTAKDAQKAGLPISVVADRQTVDALIETVSHFTLPHAADEFAADEFTS"
     gene            complement(2711..>3241)
                     /locus_tag="VN041_02805"
     CDS             complement(2711..>3241)
                     /locus_tag="VN041_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWU28005.1"
                     /translation="LERVASGELDAVILAAAGLSRLGADSALHREHLGLAEWPTAPGQ
                     GSLAVETLSDAPADLLAALAELDDEDTRIAVTVERAILAGLEAGCQAPMAAHAQVTGD
                     SIRIRSVVYEPAGTGRIGIDVTESLNGGYIRRNGSGNGASAADGADPMRAIRDIGMSA
                     AHRLLEQGAADLLPRE"
BASE COUNT          479 a         1169 c         1124 g          469 t
ORIGIN      
        1 ccgcgcgatg cgggacttcc ttgccgacct gttcgcccgt cggaatgccc gtggtagtga
       61 ccggcgaact tcaccagcag gtcgcgtccg gtggcgccgc gggccaggcg gatggcggtc
      121 atggtcgcct cggtgccggt cgacaccatg cgcacgtgct cgaccgggcg cgcgtcgccc
      181 acctgcacgc gatcggcgat cagctgcgcg agctcgacct ccccctccgt gggggcgccg
      241 aaggacagac cgcgagtcgc ggcctcctgc accgctcgca cgacctcggg gtgcgtgtga
      301 ccgagcagcg ccggccccca ggacgcgacg aggtcgatgt actccgtgcc cgcggcatcc
      361 gtcaccctgg cgccgttcgc ggatgccagg aaccggggcg tcccgcccac cgagccatac
      421 gcccgcaccg gcgagttcac gcctccgggg atcaccgcgc gggcggcgtc gaacagggtg
      481 tcattgcggt cggtcatgga tacaagccta cggatgccgg ctatgtgcgc gcctcagcgg
      541 acgccgtccc ctcgcccagc gcccgcctac ggctcagcgt ggatcagaaa cgctccagag
      601 agcgccgtgg gcggtgctct ttcgatccgc tgcggcacgc ggatgcgttg tcgatccggc
      661 ctgagagcgg cggacgggaa ccgcctggtc ggacagccgc cgacctgcga gaagcatccg
      721 caccaccggt gccgagaagc agaccccctc caggtcgcag aaaggcctgc tcactggcat
      781 cccatcctcg tccaggtcgc agaagatgcc gctcagaacg gctgtcagcg gcaccgattg
      841 cgacctggac ggcgcggtgg acggctatcc agcaccaatt gcgacctgga ccgcatcggt
      901 ccgggtctgc tcagcgggtc agcgcagcca ctcggccgct tcgaccgccc agtacgtcag
      961 cacggcgtcg gccccagcgc gccggatcga gagcagcgac tccaggatgg ccgcgcggcg
     1021 gtcgatccag ccgttcgcgg cggcggcctc gatcatcgcg tactcgcccg agacctggta
     1081 cgcccacacc ggcacgtcca cggcgtcgcg cacctcgcgc accatgtcga ggaacgccat
     1141 cgccggcttc accatcacga tgtcggcgcc ctcgtccacg tcgaacagcg cctcgcgcac
     1201 gccctcgcgg cggttgccgg catccatctg gtaggtgcgg cggtcgccct tcagctgcga
     1261 gtccacggcc tcacggaagg gaccgtagaa ggcgctggcg tacttggccg agtaggccag
     1321 cagcagggtg tcctggaagc cctcggtgtc gagcgcctcg cggatcgcgg cgacctggcc
     1381 gtccatcatg ccgctcaggc ccagcagctg cgagccggcc cgcgcctgcg cgagagccat
     1441 cgaccggtag cggtcgagcg tcgcgtcgtt gtcgacggcg cccgaggtgt cgaggacccc
     1501 gcagtgcccg tggtcggtga actcatccag gcacaggtcg gtctgcacga ccagggcgtc
     1561 gcccacttcg gcggcgaccg cggcggtcgc gacgttcagg atgccttcgg ggtcgtccgc
     1621 acccgagccg tcggcgtcgc gcacctcggg gatgccgaac agcatgaccc cgccgatgcc
     1681 ggtctcggca gcatccgccg ccgcccggcg cagcgagtcg agcgagtgct gcacgacacc
     1741 gggcatcgag ccgatcggag cgggctcgga cacgtcctcc cggacgaaca tcggcagcac
     1801 cagctgctgc gccgagagcg aggtctcgcg caccaggctc cgcaccgggg cggactggcg
     1861 cagccggcgc agccgggtct cggggaagcg ggtcatgaag tgaactcgtc cgccgcgaac
     1921 tcgtccgccg cgtgcgggag ggtgaagtgc gacaccgtct cgatcagcgc gtccacggtc
     1981 tgccggtcgg cgacgacgga gatcggcaga cccgccttct gcgcgtcctt ggcggtgcgg
     2041 gggccgatgg ccgcgagcag ggtggaatcg gggatctcgg ggaactgctc gcgcacctga
     2101 ctcgcgaccg agccgctggt gatcaggatg gcgttgatgc gaccgttctc cacatcacgg
     2161 cggatgcggt cggtgaccgg cacccccacc gtgcggtagg cgaccacgct gtcgacgtcg
     2221 tggcccgcgt cattgagcat gacggacagc acgggcttcg cgatctcgct gcgcagagac
     2281 aggacgcgac ggggctccgg ctcgagggcg atcatctgct cggccatccc ggctgccgag
     2341 ttgtcgagct ccggaacgag ggcgacctca tagcccaccg cgagcagagc agccgccgtg
     2401 gtctcgccga cggcggcgat cttcgtgcgc ttgggcaccg tgacacggtg tgcgaacagc
     2461 acgtccaccg tggtggcgct cgtgacggtc agccagtcgt agtggccctc ctgcagccgc
     2521 tcgagcgcgg catccagagc atcctgatcc gtggtggggg cgaagttgat cagcggggcc
     2581 acgacgggca ctgctccctg cgctctcagg ctcgcggcga cactgtcgcc ccacgggccg
     2641 ccacggggca ccaggacgcg ccaaccgtcc agcggcttgg ccgttttcgt ttcggaagca
     2701 gtcatcagga tcactctcgc gggagaaggt cggccgctcc ctgttcgagc agccgatggg
     2761 ctgcagacat gccgatatca cggatcgcgc gcatcgggtc tgcaccatcg gcagcacttg
     2821 ctccgttgcc actgccgttc cgacgaatat accccccgtt cagggactcc gtgacgtcga
     2881 ttccgatccg gcccgttccg gccggctcgt acacgacgct gcggatgcgg atgctgtcgc
     2941 ctgtgacctg cgcgtgggcg gccatcggcg cctggcatcc ggcctcgaga ccggcgagga
     3001 tcgcccgctc gaccgtcacc gcgatgcggg tgtcctcgtc gtcgagctcg gcgagcgcgg
     3061 cgaggagatc ggccggagca tccgacaggg tctcgaccgc cagcgatccc tggcccggag
     3121 ccgtgggcca ctcggccagg ccgaggtgct cgcggtgcag ggcggagtcc gccccgagcc
     3181 ggctcagccc tgcggccgcg aggatcacgg catccagctc ccccgacgcg acccgctcca
     3241 g
//
LOCUS       DATMSX010000093         8953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_152919,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000093 DATMSX010000000
VERSION     DATMSX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152919"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1417)
                     /locus_tag="VN041_02810"
     CDS             complement(74..1417)
                     /locus_tag="VN041_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28006.1"
                     /translation="MIRVTATPLPRSGPGWRAWLIWGVAVAAYVLAITNRSSLSAVGV
                     DTAERFHADAATLSLFAVVQLAVYGGMQIPIGVLLDRYGSRPIMVIGMLLMSVGQFVM
                     AVSPSIGVAIVVRVLLGAGDAAIFPAVIRLVATWFPAQRGPLMGQMTGLAGQCGQLVA
                     LLPLAALLHSTSWTITFSSIAGLGVLFAVLVWAFVRNRPPEQEQEQDVTVNTETGVIR
                     VVTSSIDTGVGIRAAWAHPGTRLAFWTHFTTPFAGTAFLLLWGMPFLTAGEGLSNAAA
                     AGLLSVNVIVAMVLSPILGELSRRIPTRRSLALVVPIVSVQAIGWLLVIAWPAPAPVW
                     LLTILVVCLGAGGPASMIAFDHARTHNPVHRLSTATGVTNSGGFLAGLVAIFLIGLTL
                     DLQGAGTPATYSPEAFRWAFLTQLPLWVLGLTMIIIERKRTRIRMGLDPERPRNR"
     gene            1606..2856
                     /locus_tag="VN041_02815"
     CDS             1606..2856
                     /locus_tag="VN041_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWU28007.1"
                     /translation="MLPISKRGPGGADIVDALFTATSAVCVTGLTTVDTATYWTPFGQ
                     VVIMLLIQLGGLGIMIFASLIGLVLARKLTVRSRINTASEAKATGFGDVRGLVRGIVR
                     ITLGVELSVFILLWLRFAFGYGYDPGRGAWNAGFLAVSSFNNAGFALYSDNLMGFVAD
                     PFICLPIAAAIILGGLGFPVIMQLRKEFRRPLHWTMNTKLVLWGTVVLLVGGTVYLTV
                     IEWSNPKTLGTLDPAARILAGFFQSVQTRTAGFNSIDIGAMHDASWFGMDILMFIGGG
                     PAGTAGGIKVTTFAVLFFIMLTELRGEGAVNIFGKRLSRAVHRQAITVVLIAVAAVVV
                     GTSALLLITDSTLDRVLFEAVSAFGTVGLSTGITTELPAAAKLVLILLMFLGRLGPLT
                     LGTAIALRERRVLYELPKERPAIG"
     gene            2849..3547
                     /locus_tag="VN041_02820"
     CDS             2849..3547
                     /locus_tag="VN041_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWU28008.1"
                     /translation="MVRFSLFGGDASRRIAEADSVAVIGLGRFGSALAHELMAAGTEV
                     LGVDLDEDLVQAHNGELTQVVRADSTREEALRQLAIDEFERVVVAIGDDVSASILTCS
                     VLLSMRIPVIWAKAVDDRHGLILEQLGVHKVIYPEHDMGRRVAHLVRGAALDYIEVAP
                     GYALVKAAAPSMLHGVALGETGVRTTHGITVAAYQHGADEWHNADNTTVLQPGDTILI
                     VGPTERAEAFAQLR"
     gene            3605..4687
                     /locus_tag="VN041_02825"
     CDS             3605..4687
                     /locus_tag="VN041_02825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWU28009.1"
                     /translation="MTPAPWVLHVDMDQFIAAVEVLRRPELAGRPVIVGGNGDPTERA
                     VVSTASYEARAFGIGSGMPLKIAARKAPEDAVFLPVDHAAYEEASAGVMSALRALPGV
                     VVEIIGWDECFLGATTDAPEALARDAQHAVLTATSLHCSVGIGDNKVRAKIATEFGKP
                     RGVFRLTAQNWFEVMGERPTRDLWGVGSRVQKRLADHGIRTVRELADADEAEMVREFG
                     PKMGIWYHGLGSGRGPAVVDDTPWIARSHSRETTFQQNLTTRAEIENAVEDLAGHAFD
                     DCIAEGRPVIRVHLKVRYAPFETRTTGRKLSAPTQDRDAFIAAARELADGLDTDREVR
                     LLGVRAEMTMPEGGDPGERTPVRGRI"
     gene            4791..5240
                     /locus_tag="VN041_02830"
     CDS             4791..5240
                     /locus_tag="VN041_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28010.1"
                     /translation="MTAAVRVTTDLDDIDLDVVHRWLSEEAYWALGRSREVVEAAARA
                     SVNFGALDADGRLVGYARVVTDQVTFAWLCDVFVDPAARGLGAGKALAGAVVAAVRPM
                     GLKRVMLATADAHALYRRFGFEGVADPEKLMILAQPSAQRGADQEAV"
     gene            complement(5210..5857)
                     /locus_tag="VN041_02835"
     CDS             complement(5210..5857)
                     /locus_tag="VN041_02835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU28011.1"
                     /translation="MNLFEVLARFNAAHPWSHNDAYAGFVLRHARAVRRSGGDVAVDV
                     GCGTGNLLARLTEVFPTVIGVEPDARSGAVARARFPRADVRVDIRSFGDEPEAAYDLL
                     VFVASLHHMPVRQALSAARSSLRPGGRLVIVGIAKETRADSFRSSVSMVLNPLIGMVR
                     HPRRATHLPVSMEAPVAEASQTFEEIRDMASAMLPGIRMRRRLFWRYTASWSAPR"
     gene            6025..6513
                     /locus_tag="VN041_02840"
     CDS             6025..6513
                     /locus_tag="VN041_02840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWU28012.1"
                     /translation="MWNHEPADYDCPFCRIQGGAYDDNNRAEDIVAVTPLAYARVAPK
                     WWPDNQGSLLVIPRGHHENLYDLPQQVNHAVWDLVQQSARAVRAAYGCQGTSVRQHNE
                     PAGDQDVWHLHVHVFPRWTGDQLYTRHHEATWHAPAERAAYAAKLIPALGLPTRFEDT
                     AG"
     gene            complement(6638..7480)
                     /locus_tag="VN041_02845"
     CDS             complement(6638..7480)
                     /locus_tag="VN041_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWU28013.1"
                     /translation="MSAIDAEDLGAYGVHVLIGDDEAQHRWRSDDRVNIYSVSKGVSA
                     LAAGMAADAGILTLDTRVAELLPTMRFGEGVDHVTLRHLLTMTSGIDFAWFGSQEAPW
                     PDIAQEMLRRPSRGVGEVYQYSDASTYVAMRMLGAVVGDVRDWLMPRLFEPLGIHNPQ
                     WHRCPLGWIVGGTGLELRTEELSRIGRVLRDRGAWHGAQLIGAEWIDAMHADWVRTGW
                     EGDSSQYGIAVWAGPGDGWRLDGLYGQYVYVDRSRHAVVTITAHEETRDHRLVQIAST
                     VLAG"
     gene            complement(7607..8404)
                     /locus_tag="VN041_02850"
     CDS             complement(7607..8404)
                     /locus_tag="VN041_02850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU28014.1"
                     /translation="MTSADQQDIDARIQTYYGEVFDEHVRLTTRSAQGPLEFQRTQEI
                     IRSHVPDGRVLDIGGGAGIHSRALMDAGYEMEMIDPVVRHVEQAQEAGVRASVGDARE
                     LEYADQSFDAAILLGPLYHLASREDRLRALREAARVVRAGGVVLAAGLSRYVAFGAAS
                     LGRPVPTPIPGEWTALIADGTPGSQLRFPAGHFHTAEELEDEVADAGMDVLEVVGVEG
                     PAGLLLESVADVHPDAREAALTVARAASAVPGIRDQSAHLIAVARVL"
     gene            8658..>8953
                     /locus_tag="VN041_02855"
     CDS             8658..>8953
                     /locus_tag="VN041_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWU28015.1"
                     /translation="MSRPPRTLATELGTAAPGTTVTLMGHVHRRRILSSVTFLVLRDR
                     TGLAQVVLHRDAEHPADVPPEETVVRITGLVAANPQAPGGAEVVSPQIEVLGEPA"
BASE COUNT         1468 a         3046 c         3083 g         1356 t
ORIGIN      
        1 gcggcggccc gcatccgatc ggctggacgc gacgcggctg agcgacgcga cccacccccg
       61 ggcatagcgg cggtcagcgg ttccgcgggc gctccggatc gagtcccatc cggatgcggg
      121 tgcgcttgcg ctcgatgatg atcatcgtca gtcccaggac ccacagcggc agctgggtga
      181 ggaacgccca ccggaacgcc tcgggcgagt aggtcgccgg ggtgccggcg ccctgcagat
      241 cgagcgtcag gccgatgagg aagatcgcga cgagcccggc gaggaatccg cccgaattcg
      301 tcacgccggt ggcggtgctg agccggtgca cggggttgtg cgtgcgggcg tggtcgaagg
      361 cgatcatcga cgccggccca ccggctccca ggcacacgac caggatggtg aggagccaca
      421 ccggggccgg tgcgggccac gcgatgacga gaagccatcc gatggcctgc acgctgacga
      481 tcgggacgac cagcgcgagc gaccgccgcg tggggatccg gcgggagagc tcgcccagga
      541 tcgggctgag caccatcgcg acgatcacgt tgaccgacag gagtccggcc gccgctgcgt
      601 tggagagtcc ctcgcctgcg gtgaggaacg gcatacccca cagcagcagg aaggcggtgc
      661 ccgcgaacgg cgtggtgaaa tgcgtccaga aggcgagccg ggtgccggga tgggcccagg
      721 ccgcgcggat gccgacaccg gtgtcgatcg aggaggtgac gacgcggatc acgccggtct
      781 cggtgttgac cgtgacatcc tgctcctgct cctgctccgg gggacggttg cgcacgaatg
      841 cccacaccag gaccgcgaac aggacgccca gtccggcgat gctgctgaac gtgatcgtcc
      901 aggacgtgga gtgcagcaga gccgcgaggg ggagcagtgc gacgagctgg ccgcactggc
      961 ccgccaggcc ggtcatctgc cccatcagcg gaccgcgctg ggcggggaac caggtggcga
     1021 ccaggcggat caccgcgggg aagatcgccg cgtcgcccgc gccgagcagc actcggacga
     1081 cgatcgcgac gccgatgctg ggggagacag ccatcacgaa ctgccccacg ctcatgagga
     1141 gcatcccgat gaccatgatc gggcgggacc cgtagcggtc caggagcacc ccgatcggga
     1201 tctgcatccc gccgtagacg gccagctgca ccaccgcgaa cagcgacagg gtggcagcat
     1261 cggcatggaa ccgctcggcg gtgtcgaccc cgactgcgct cagcgagctg cggttcgtga
     1321 tggcgaggac gtacgccgcg acggcgacac cccagatcag ccaggcgcgc cagccggggc
     1381 cggaacgagg gaggggagtg gcggtcactc gtatcaggat actcggcgga gacaggcgag
     1441 acggtgcgcc cggtgtacag ttgcccggtg gatctgaaag ggcagggcgc tgcagcgccg
     1501 gggcgcgcac gccgcagtcc ggcccgcagc cgggctggcc tgcacccgtc gcaggtggtc
     1561 gtactcggat tcgcctcggc gatcgtcgtg ggcacggcgc tgctcatgct cccgatctcg
     1621 aagcggggcc cgggtggggc cgacatcgtc gatgcgctct tcaccgcgac atccgcggtc
     1681 tgcgtcacag gcttgaccac cgtggacacc gcgacctact ggacaccgtt cgggcaggtc
     1741 gtgatcatgc tgctgatcca gctgggcggt ctcggcatca tgatcttcgc gtcgctgatc
     1801 ggcctggtgc tggcccgcaa gctgacggtc cgatctcgca tcaacacggc ctccgaggcg
     1861 aaggcgaccg gattcggaga cgtccgcggc ttggtccggg ggatcgtccg gatcacactg
     1921 ggagtcgagc tgagcgtctt catcctgctg tggctccggt tcgccttcgg gtacgggtac
     1981 gacccgggca gaggagcctg gaatgcgggc ttcctcgcgg tctcctcgtt caacaacgcg
     2041 ggcttcgcgc tgtactcgga caatctgatg gggttcgtgg ccgatccgtt catctgcctg
     2101 ccgatcgcgg ccgcgatcat cctcggcggc ctcgggttcc cggtgatcat gcagttgcgc
     2161 aaggagttcc gccgtccgct gcactggacg atgaacacca agctcgtgct gtgggggacc
     2221 gtcgtgctgc tggtcggcgg aacggtgtat ctgacggtca tcgaatggag caatccgaag
     2281 acactcggca cactcgatcc ggcggcacgg atcctggctg ggttcttcca gtcggtgcag
     2341 acccgcacag ccggcttcaa ctcgatcgac atcggagcca tgcacgacgc cagttggttc
     2401 ggcatggaca tcctgatgtt catcggcggc ggaccggcgg gaacggcggg cggcatcaag
     2461 gtcacgacct tcgctgtgct gttcttcatc atgctgaccg agttgcgcgg cgagggcgcg
     2521 gtgaacatct tcggcaagcg gctcagccgc gccgtgcacc gccaggccat cacggtggtg
     2581 ctcatcgccg tcgccgcggt ggtggtcggt acctcagctc tgctgctgat cacggacagc
     2641 accctggacc gggtgctctt cgaggccgtc tcggcattcg gaaccgtcgg gctctcgacg
     2701 ggcatcacca ccgagctgcc cgctgcggcg aagctggtgc tgatcctgct gatgttcctc
     2761 ggtcgactcg gtccgctcac cctcggcacg gcgatagcgc tgcgtgaacg tcgcgtgctc
     2821 tatgaactcc ccaaggaaag gcccgccatt ggttaggttc tcgctcttcg gcggcgatgc
     2881 gtcacggcgc atcgccgagg cggactcggt agcggtgatc ggtctcggcc ggttcggaag
     2941 cgcgctggcg cacgaactca tggcggccgg cacggaagtg ctcggcgtcg atctcgacga
     3001 ggatctcgtc caggcgcaca acggcgagct cacccaggtc gtccgggccg actccacccg
     3061 ggaggaggcg ctgagacagc tcgcgatcga cgagttcgag cgggtggtcg tggcgatcgg
     3121 agacgacgtg tccgcctcca tcctcacctg ctcggtgctg ctgagcatgc ggataccggt
     3181 gatctgggcg aaggccgtcg acgacaggca tggcctgatc ctggagcagc tcggcgtgca
     3241 caaggtgatc taccccgagc acgacatggg gcgccgtgtc gcgcacctgg tccgcggtgc
     3301 ggcgctcgac tacatcgagg tggcgccagg ctacgctctc gtcaaggcag cggcaccctc
     3361 catgctccac ggcgtcgccc tcggggagac cggagtccgc acgacccatg gcatcactgt
     3421 cgccgcgtac cagcatggcg cagacgaatg gcacaacgcc gacaacacca ccgtcctgca
     3481 gccgggtgac acgatcctca tcgtggggcc caccgagcga gccgaagcgt tcgcgcagct
     3541 gcgttgagac ggcggccgca agggtcctgg tgacagatcg ggcttcgtgc cgcagactcg
     3601 aggcatgact ccggcgccct gggtgctcca cgtggacatg gaccagttca tcgcggcggt
     3661 cgaggtgctg cggcgccccg agctcgccgg acggccggtg atcgtcggcg ggaacggcga
     3721 tcccaccgaa cgggccgtgg tgtccacggc gtcgtacgag gcgcgtgcgt tcggcatcgg
     3781 ttcggggatg cctctgaaga tcgccgcccg caaggcgccc gaagacgcgg tcttcctgcc
     3841 cgtggatcac gcggcgtacg aagaggcatc cgccggggtg atgagcgcac tccgcgctct
     3901 tcccggcgtg gtcgtggaga tcatcggatg ggacgaatgc tttctgggag cgacgacgga
     3961 tgccccggag gcactggccc gcgatgcaca gcacgccgtg ttgacggcga cctccctgca
     4021 ctgctcggtc ggcatcggcg acaacaaggt gcgcgccaag atcgcgacgg agttcgggaa
     4081 gccccggggc gtcttccgac tcaccgcgca gaactggttc gaggtgatgg gggagaggcc
     4141 cacccgcgat ctgtggggtg tgggctcccg cgtgcagaag cgcctggccg accacgggat
     4201 ccggacggtg cgcgaactcg ccgatgccga cgaggccgag atggtccgcg agttcggtcc
     4261 gaagatgggc atctggtatc acgggctcgg ttccggacga ggtccggcgg tggtggacga
     4321 cacgccgtgg atcgcccgca gccacagccg ggagacgacg ttccagcaga acctcacgac
     4381 gcgcgccgag atcgagaacg cggtcgagga cctcgcaggc cacgccttcg acgactgcat
     4441 cgcggaggga cgacccgtca tccgcgtgca tctgaaggtg cgatacgccc cgttcgagac
     4501 acgcacgacg ggccgaaagc tgtccgcgcc gacgcaggac cgcgatgcgt tcatcgcagc
     4561 cgcgcgcgaa ctggccgacg gcctggacac cgatcgcgag gtccggctgc tgggcgtccg
     4621 cgccgagatg accatgcccg agggtggtga cccgggggag aggacgcccg tgcgcggccg
     4681 catctgagac gggtggctcg gataccgggt ctgcgcccgc gggcaccgcc tcgcgagcca
     4741 tcggcacggg cttgcgctgc gcagtgacgg gcggccttag gctctgaccc atgactgcgg
     4801 cagtgcgcgt gacgaccgac ctcgacgaca tcgacctgga tgtcgtgcac cgctggctct
     4861 cggaggaggc gtactgggca ctcgggcgat cgcgtgaggt cgttgaggcc gcggcacgcg
     4921 cctcggtgaa cttcggagcc ctggatgccg acggccggct cgtcgggtac gctcgcgtcg
     4981 tcaccgatca ggtcacgttc gcctggctct gcgacgtctt cgtcgatccc gcggcccgtg
     5041 gtctgggcgc aggcaaggcc ctggcgggtg ctgtcgtcgc ggctgtgcgg cccatgggcc
     5101 tgaagcgtgt catgctcgcc accgcggatg cccacgcgct ctaccgccgg ttcggcttcg
     5161 agggcgtcgc cgaccccgag aagctgatga tcctcgcgca gccgtctgct cagcgcggcg
     5221 cagaccagga ggcagtgtag cgccagaaaa gccgacgccg cattctgatt ccaggcagca
     5281 tcgcagatgc catgtctcgg atctcctcga acgtctgcga cgcctcagcg accggggctt
     5341 ccatgctcac cggaagatgc gtcgcacggc gcggatgccg caccatcccg atgaggggat
     5401 tcagcaccat cgacaccgag gagcggaacg agtctgcccg ggtctccttc gcgatgccga
     5461 cgatcaccag tcgtccgcca gggcgcaggg aagagcgcgc ggccgacagt gcctgccgca
     5521 ctggcatgtg gtgaagcgaa gcgacgaaga cgagaagatc gtaggcggct tccggttcgt
     5581 cgccgaagct ccggatgtcg actcgaacgt cggctcgcgg gaaacgcgct cgcgcgacgg
     5641 cacccgagcg ggcatcaggt tcgacgccga tcacggtggg gaacacctcg gtgaggcgcg
     5701 ccagcaggtt tccggtgccg catccgacgt cgacggcgac gtccccgccg gagcgtcgca
     5761 ccgcgcgggc atgacgcagc acgaatcccg cgtatgcgtc gttgtgagac cagggatgcg
     5821 cggcgttgaa tcgggcgagg acctcgaaga gattcatcgg tcggaccccg caggtatctc
     5881 gcatggagcc gccctgccac gacggttcag atcgtctgga agcatcgccc ggccgtgcac
     5941 gcccataggg tgatcctagg cgtcgcgacc gcggcgctcc gcgcccgcgg ccccttactg
     6001 ggggagaggt gaggtcatcg acgtatgtgg aaccacgagc cagccgacta cgactgcccg
     6061 ttctgccgca tccagggcgg ggcgtacgac gacaacaacc gcgcggagga catcgtcgcc
     6121 gtgacgccgc tggcctacgc gcgcgtcgca ccgaaatggt ggcccgacaa ccagggcagc
     6181 ctgctggtca tcccgcgcgg acatcacgag aatctgtacg acctgccaca gcaggtcaat
     6241 cacgcggtct gggatctcgt ccagcagtcg gctcgcgccg tgcgtgcggc atacggctgc
     6301 cagggaacgt cggtgcgcca gcacaacgaa ccggcgggcg accaggacgt ctggcatctc
     6361 cacgtccacg tgtttccgcg ctggacgggc gaccagctct acacgcgcca tcacgaggcg
     6421 acgtggcacg cacctgcaga gagggctgcg tacgcggcga agctcattcc ggcgctcgga
     6481 ctgcccacgc gtttcgagga cacggcgggc tgagcggggc tcaggggatc gaagcgagcc
     6541 tctctccttc acttgttctg acataatgtg cattatcggt tattgcccga tggcacacac
     6601 agctcgagag gggctccacg accgcgtccc tgccgactca gccagcgagc accgtcgatg
     6661 cgatctggac gagccgatgg tcgcgcgtct cctcatgcgc tgtgatggtg acgaccgcat
     6721 gtcgcgatcg gtcgacatac acgtactggc cgtacaggcc gtcgagtcgc cagccgtcac
     6781 ctggacctgc ccagacggcg atgccgtact gcgacgagtc gccttcccat cccgtgcgca
     6841 cccagtccgc gtgcatcgca tcgatccact ccgcgccgat gagctgagca ccgtgccatg
     6901 ctccgcggtc gcgcagcaca cggccgatcc gagagagctc ctcggtccgc agttcgaggc
     6961 cggtgccgcc gacgatccag ccgagtgggc aacgatgcca ttgcggattg tggatcccga
     7021 gtggttcgaa cagccgcggc atcagccagt cacgcacgtc gccgacgaca gccccgagca
     7081 tgcgcatggc gacgtacgtg ctcgcatcgg agtactgata gacctcgccc acgccgcgcg
     7141 aaggacgacg gagcatctcc tgagcgatgt cgggccacgg cgcctcctgg ctgccgaacc
     7201 acgcgaagtc gatgccgctc gtcatcgtga gcagatgccg cagcgtcaca tgatccacgc
     7261 cctcgccgaa ccgcatcgtc ggcagaagct ccgctacacg cgtgtcgagt gtgaggatcc
     7321 ccgcgtcggc ggccatcccc gccgccagcg cgctgacacc cttcgacacg gagtagatgt
     7381 tcacccggtc gtcgctgcgc cagcgatgct gcgcctcatc gtcgccgatg agcacgtgga
     7441 cgccgtacgc gccgaggtcc tcggcgtcga tcgcgctcac gattcggtcc cgtgcggtcg
     7501 ccgctgacat cactcgttga ggctagcagc gagctccgac accggccctg gttctggacg
     7561 atccaggggt ctccgttgcg cccactcccc tgattcgagg cgcttgctat agcacccgcg
     7621 cgaccgcgat cagatgcgca ctctggtccc ggatcccggg aaccgcagac gccgcccgcg
     7681 cgacggtgag cgcggcctcc cgcgcatccg gatgcacatc cgcgacgctc tcgagcaaaa
     7741 gccccgcggg accctcgacg ccgacgacct cgagcacatc catgccggca tccgcgacct
     7801 cgtcctcgag ttcctccgcg gtgtggaagt ggccggcggg gaaccgcaat tgcgagcctg
     7861 gcgtcccatc cgcgatgaga gccgtccact cccccggaat cggcgtcggc acgggtcgtc
     7921 cgagagaggc cgctccgaac gcgacatatc gcgaaagtcc cgcggccagc accacaccac
     7981 ctgcgcgtac cacccgagcg gcctcacgga gcgccctgag ccgatcctcc cgcgaggcga
     8041 ggtgatagag cggacccagc aggatcgccg cgtcgaagga ctgatccgcg tactccagct
     8101 cgcgcgcgtc gcctacagag gcccgcacac cggcctcctg cgcctgctcg acgtggcgga
     8161 cgacgggatc gatcatctcc atctcgtagc ccgcgtccat cagggccctg gagtggattc
     8221 cggcgccgcc gccgatgtcg agcacacggc cgtccgggac atgagagcgg atgatctcct
     8281 gcgtgcgctg aaactccaga ggtccctgcg ccgaccgagt cgtcagccgg acgtgctcgt
     8341 cgaagacctc gccgtagtac gtctggatgc gcgcatcgat gtcctgctgg tcagcggatg
     8401 tcatgccgtc gaggctagct gttagccgcg cccgcgcgag caatgcgcgc acgatgtcgg
     8461 cccttccgcg agtgatgcta acgtgttgcc tggcagacgt gaggcgtgac gaccctcgcg
     8521 ccggattcgc tccggcagac ggctcccgcg cgtgacggcg taccgacgag aggccggcga
     8581 ctcaccggcg aaggtgtggt ggcaccgcga tctggccccc gcccacacct ccagggctcc
     8641 atgcacctgg aggaatgatg agcagaccac cccgcacact cgccactgag ctcggcaccg
     8701 ccgcacccgg caccacagtc accctgatgg gtcacgtgca ccggcgtcgc atcctgtcgt
     8761 ccgtgacctt cctggtgctg cgcgaccgca cgggcctcgc ccaggtcgtg ctgcaccgcg
     8821 acgcggagca tcccgccgac gtgccgccgg aggagaccgt cgtccggatc acgggcctcg
     8881 tggcggcgaa cccgcaggcg ccgggcggag cagaggtcgt ctccccgcag atcgaggtgc
     8941 tcggcgagcc ggc
//
LOCUS       DATMSX010000094        23129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_86658,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000094 DATMSX010000000
VERSION     DATMSX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 23129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23129
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86658"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VN041_02860"
     CDS             complement(<1..575)
                     /locus_tag="VN041_02860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWU28016.1"
                     /translation="MAEYPGAAWAGVSANKSPRQGKVRLFIVHHMASTQSAASARTRF
                     MGSNDRSVSPNYQVNADGSVYEIVPPDHYRAWTTGAVDHQAVTCETQNTSGAPSWGIS
                     RASMEAIAHLVAWASKRYGFPIQRGRVTGTTNANVVEVPGVVGHNETPAGRRTSTACP
                     GPSMDISWIIARAQQIAGTGGGPATTPAPRPS"
     gene            577..2793
                     /locus_tag="VN041_02865"
     CDS             577..2793
                     /locus_tag="VN041_02865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWU28017.1"
                     /translation="MGSFHDEAPSVDGAQVFEGGLGPIGSRWGRHTGGSVRSSRRQRT
                     KTVRKDIQGLRALAVLLVFANHIVGWPTGGFAGVDVFFVISGFLITGLLLRDYERTGT
                     ISPRKFYLGRLKRIVPSATVVLLFVIGFSWALFTKTRAVSVTWDAFWAFIFGSNWNYA
                     IAGTDYFNQDAAVSPLQHYWSLSVEEQFYFVWPWLLLGLLVVFARFITVDGRRARQIA
                     ATTISIIVIASFAWAMYSGATQPTVAYFSTFTRAWELGIGALVAIAAPLLSNIPRIAR
                     TVLAYVGLVGIIASCVLIPTTAAWPAPWALLPVLATATVIVSGIGEEARFIQPLTNPV
                     SVYIGDISYSLYLWHFPAIIFGHALYPQAGSLAYLGIVLAGFALATAQYYAIERPIWK
                     SPFGTGARSGDWKRWWNDHSLPVQRGLVGGMIAMSAAVIGVVFVPTPAPVDSPAAAAT
                     PQPISTAIDETPAVAAVNQSVRSALSLTTWPELNPSIDEVGPNSKVDAWVNDGCLALE
                     KGAGRDPLSTLKRCTYGPAGGERIALVGDSIAISWLPAIQAAYPSAEIRVMTMQQCPF
                     AAVSVKKGDGSSLPECDAFHEAVNAQLAELKPTLTVLSQAENSAARTTGDTDLTAGIA
                     EAVQSATAVSDRVVILPPPPQGRNISDCYSAQGTPSACTVDTNAAHRAAIKALKDAVE
                     ATPEVRVIDTDGLFCLAGRCPAIADGTIIRADVAHLTQQYSAKLGPALAELLRPEG"
     gene            complement(2813..5008)
                     /locus_tag="VN041_02870"
     CDS             complement(2813..5008)
                     /locus_tag="VN041_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28018.1"
                     /translation="MAFQDYFGTPIAYDPTQDRLVADAAFTVHATDDTSLSTPLTVLE
                     PGSGATIPELRSSSIGVLPDFRVDGNPPQVLIKSGPFVTKLTSVYGAILAAGLDPDTV
                     AAAIQAGADATAARDVATSAADDAVAAAAAATAVGTTNDAIMAGVAADQGSEFLGQMS
                     ATFAGRWQANRHYAAGAVVITPAGNLVRRLTEGNAAASWTATEAAQWVGTRGEIFATD
                     FGVTADGTTDDGAAMVSALATAATLQVTLRLPGGKVKVNSLLTWKHRLHDIEGGSNTI
                     LDFSGISTNNVTVFTLDPTAFVSDIAQSKWSVCRGVHFLGSLSNFTNTHGITHLTTAD
                     TVNVRFENCSVQGFKNQVVLKDNAYLWKFSNCSFRYAMGRTFSFGGLNMGENFHFEHC
                     KIFNNNNDTFVYTAPTNAGDIKFSQCSFDGGAQVADLNGGTVNWDDCHFEGTKYGPQW
                     VKMTSGGSASTVAKFTGGQLYISTRTDSVRHVLFRIGAPLGGSTNPVSLSVDGLHIID
                     RSNSAGAILVKDESGLTHPWGASANLIKMRPQIAPFSPPQEIAIGSASQWLGGALAAL
                     PTVGTGTAQPHQAQFRSSYSTSTTPLAISFDSAKTNRDGIAGAVLFSSATTSGTGTFL
                     ARRFLCRAGRQMAVSVSFARENLLIGTGGMDMRVSWWADTSATTAISTGQLVATLIPQ
                     ATTADTVGWRTVMLVLNPPPGALLGEVWFGNAAMSGKIWVQDFDILELT"
     gene            complement(5024..5827)
                     /locus_tag="VN041_02875"
     CDS             complement(5024..5827)
                     /locus_tag="VN041_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWU28019.1"
                     /translation="MATNRITSNQGPSPYASFYLDCDVIQTDTAGMRWLLRAYLRMSK
                     GSSSSYGGSGFQSVQGAPGEIARYPRSPFLPSGITSWTQGPYDWWVPANGSGFWSGTS
                     TTYPLGMQLSYGNVQTTAYGSIQLSQIATTPPAPKPLGVTNVTQTSARYSFSSQGLGG
                     GTFLRWEAQWSTSPTFATGNSPIVTSSGTTDFTDLPPGTKVYFRSRGVNSLGVGAWSQ
                     IISATTLPSGARVGVGGAFPIASTAVGVAGGFVPAQILIGKGGVFVTPA"
     gene            complement(5841..6470)
                     /locus_tag="VN041_02880"
     CDS             complement(5841..6470)
                     /locus_tag="VN041_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28020.1"
                     /translation="MITSTGFEGTVDYAEWSQLTSHMGAQYGVIGKDSFAVAPGTGDR
                     VLSVQPGTAAGQGILDESDAVESLVGAPVASGNRWDLVALRRQWGAKASTLVLIQGGS
                     AASIPARSTTPGVEDDQPLALVRFSAGQTAAQEVIDLRVWHGDGGLAARHALVRDYLT
                     RIGTRIWINGVTWVIGFDASGNQTWVPDSVYVGSTQPPYADNLVWVKKP"
     gene            complement(6470..7564)
                     /locus_tag="VN041_02885"
     CDS             complement(6470..7564)
                     /locus_tag="VN041_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28021.1"
                     /translation="MDDEIRFVVYDKAGTFRRTVVPRLSTAEIPANSTPWAELTLDDD
                     HDALPALTADGARCAYWFRGIEWFRGRVSATPGNGPVGSVTARVEGDFRKLTEWQGWP
                     KPTAAITAQDVDYARYTGSSEDVFKAALAANFSRLGVPWTVAASLGRGSAARAEFRFH
                     PLAEKTIPALDADDLIVTIGYAPDPVVDVRASALVPGLLSDLTGALEDYDWTRNAPSA
                     TRVVVGGAGEGAARELYQQIDTAREADWGDIIETFKDSRMADAGADFSIDAAEALAEG
                     APTVGVSMQLQESVKFRLGTTFLPGDRVRVKVGPLDLIERITLARVEDSPDQGVVVTP
                     HLGSIEDSPDAQLGAQVAGLARGLRDAGRR"
     gene            complement(7577..8449)
                     /locus_tag="VN041_02890"
     CDS             complement(7577..8449)
                     /locus_tag="VN041_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28022.1"
                     /translation="MGSELSALGYRWIGHDGSVWDLNDPESGLFLDNSGVTGLHFPKV
                     DKFRSTSRAVPGFRSRGWRAASRDVFWPVCIWADGSETWRSLYSRFFATIHPSKAGVW
                     EVTAGTQTRRLRLTGRFEEQHQFALDPYMEGWAIIPVQMEPEQPFWEGKTIRRGPFSA
                     PTGVDFIPPTLAPSFHISPAATFASATIPNFGDVDAYLVWTVEGPLTDVVLGVGGVTA
                     TVPFSLAAGQVLRIDTDPRNVSALLDGVDATEPLGLLEFAPVPSGGSAPLTVSASGAG
                     GISAELTPLYFRAM"
     gene            complement(8492..11377)
                     /locus_tag="VN041_02895"
     CDS             complement(8492..11377)
                     /locus_tag="VN041_02895"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HWU28023.1"
                     /translation="MATSAEIANAYVSITTRMPGVKNDITKTLGGADVSAALKGSGKS
                     MGQRLVDGFAPIAKVGLAAVGAVAAAGLGAALVKGFKRLDAIDTASAKLRGLGHDAAT
                     VDTIMANALASVKGTAFGLGDAATVAAQAVAAGVKPGKDLEGVLSTVANAAAAAGTDL
                     GEMGSIFSKAMTQANGVQNDVIGQLADRGIPIYQELGKVLGVTSDKVFKMASEGKINF
                     DQFAAAATAASGTVASEMGGTWSGLVDNMMASLGRIGEGILRGLFPEMKNGVADLTTV
                     FSGFEAKSKEVGAAIGTFFTWIKENKDLVGILASSIGTLVAVVTAVYTGVKIWTAVQA
                     ILNVTLMANPLGAIIMGISLLVGAIVWIATQTTWFQDIWREFTRFLGEAWANISGFFV
                     AAYENVIRPTFEGIGAAATWLYETILKPVFDGISAVVGFVAGVIKFNFDLVVNYFRFW
                     GAVAGWLWKNAIGPALDAIGAAFGWLWSTIIKPVIDAIGIALQVMYAVFVKPVLDAIG
                     IGLKVLGDVFTWLNVNVVQPVWTAISDAMKAAWNWIDTNVFTPFKKGIDLIAKGFEIA
                     KSSIEKTWDGIKKAAAVPINFVLDTVWNNGLRSFWNDLVTELGLKDMKLAKAPLVKFA
                     SGGVLPGYTPGRDVHQFFSPTGGRLALSGGEAIMRPEFTRAVGGPAGVDMLNRMARTG
                     QAFKNGGVFGELGNFAGDVWDNIVSAASTVGDFLTDPVKAIQKHIVDGIIRPLMGGGG
                     NIFMKTVGQMPINLVKNLAKVFEGGPGKGVPGMGWQAMWNIVHNALPGARLTSGFRSA
                     AQNAAANGAKGSYHMRGRAIDVVPASMATFNAIRSLFPNASELIYTPAGAAQLLNGKP
                     FSGWSPKVKAQHYSHVHLAMANGGVVPKLYDQGGWLPHGGMAVNQSGKPEAVLTPEES
                     QALRAGVAGGVTINGDIYTRDEAELIERLRREQRAALYRSGALVKVG"
     gene            11431..11775
                     /locus_tag="VN041_02900"
     CDS             11431..11775
                     /locus_tag="VN041_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HWU28024.1"
                     /translation="MSIIEAAGRNGQIHFDGKTVTIRREGFFGRAGHGRGEKMIPIRQ
                     IAGVQMKPSGALTNGFIQLAVPGEISTTKALKGGRTMDAANDENAVIFTKKQQPQFEA
                     LRDAIRDAVAAL"
     gene            complement(12158..12592)
                     /locus_tag="VN041_02905"
     CDS             complement(12158..12592)
                     /locus_tag="VN041_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28025.1"
                     /translation="MADANEVESTSVPKGAKQPQDRKPKDDKPKVDQVEIDTPTGEVD
                     DEGKPKTRKVKASRVAIRGIVVTVPHEALDDFEVLDDIRAAQDSDDASRMPALLRRLV
                     GDVDYKRVLNALRGANGRVSIADGGQFVMDLFEALGQGQGNS"
     gene            complement(12595..13152)
                     /locus_tag="VN041_02910"
     CDS             complement(12595..13152)
                     /locus_tag="VN041_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28026.1"
                     /translation="MGVNAELARIFGSDIDAIHLAPLGTTLPTDIDGVLDPAFEDVGW
                     LSDDGVTESLTGSVEKKRGHQGNGVVRTRISEPGTTVTFSALESKAQTMGLRYVEKTS
                     TVTAGVRKTTRGPGQRITARAAVLDFYDADETTVKERYIIPRLEIAPNGDRVLNNADI
                     ATLPFIGEIIGEYTHLATDLEETGA"
     gene            complement(13180..13593)
                     /locus_tag="VN041_02915"
     CDS             complement(13180..13593)
                     /locus_tag="VN041_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28027.1"
                     /translation="MPDVEFADAEAMVGAFLRSRLGVPVSTKVPNPRPARFARAWRTG
                     GGTDNRAVETVQITVTCFAADSVTASNDAAKAKHLLLNEYTAMPLVRGVTIQAGPYFD
                     PDPDTNGDRYTVTAVMRVRARRDQAAAPAATTAAG"
     gene            complement(13580..13828)
                     /locus_tag="VN041_02920"
     CDS             complement(13580..13828)
                     /locus_tag="VN041_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28028.1"
                     /translation="MTDVRVQLKLRGINHVMTSPGAVAAVVAAANSIQREAGPEFEVN
                     VVPHRYTARAFVRAKSAKGRRQEARDKVLTKAVGHAGR"
     gene            complement(13832..14161)
                     /locus_tag="VN041_02925"
     CDS             complement(13832..14161)
                     /locus_tag="VN041_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28029.1"
                     /translation="MRQVSEDVIWMPFVAGAVDAHGNPIESWGDPVTKGIYALDPGST
                     SEPREGQDRVIVEPAVYMPTDVVFGSRDLVIARGNAYTVEGVTREWRHPRLGRIGNVA
                     TLRRVEG"
     gene            complement(14158..14583)
                     /locus_tag="VN041_02930"
     CDS             complement(14158..14583)
                     /locus_tag="VN041_02930"
                     /inference="COORDINATES: protein motif:HMM:NF020912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp19/Gp15/Gp42 family protein"
                     /protein_id="HWU28030.1"
                     /translation="MASWTGPTDVTGAWIGEGAPTDTGKIQKWIDKAEREIRFRVPDI
                     QTRIDAEAAETTARTDLLELAKDVTVAMVMRVFRNPEGIRQANTTTGPFTDSVTYGGD
                     VPGGLGLTDDELAKLQGARGGAFSIDLIPSTSPFYVGPS"
     gene            complement(14650..14811)
                     /locus_tag="VN041_02935"
     CDS             complement(14650..14811)
                     /locus_tag="VN041_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28031.1"
                     /translation="MAEKKQVRLTSTGGTVVRVSEDEAKMLGGEWKAEKASAKTATKS
                     AEKASAKTE"
     gene            complement(14827..15729)
                     /locus_tag="VN041_02940"
     CDS             complement(14827..15729)
                     /locus_tag="VN041_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HWU28032.1"
                     /translation="MAITAATKLSDFSGFLTREQSQPIFERAARSSTVQSLAPEVPLG
                     GNGKSIPVVTGRLTANWVAEGAQKPASKGALALKNMDPKKIATIAVVSAEVVRANPGN
                     YMSLIRNQVGDAFAEAFDAAALHGTNTPFSTYIAQTTKSVELGTTTQANGGIFGDINA
                     GLALLVNDGKRLTGFALDDRFEPKLNGAVDTAGRPIFIDSPLVENAGPIRQGRLLGRN
                     AFIGEGVYDATTKTYGFGGDWSQAAWGVVGGISYKVSTEATVTINGELVSLFENNLVA
                     VLAEAEYGFLVNDTAAYVEYVDAA"
     gene            complement(15753..16244)
                     /locus_tag="VN041_02945"
     CDS             complement(15753..16244)
                     /locus_tag="VN041_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28033.1"
                     /translation="MTTETAPATPAPAAPEAPAQGATPDEPLGTPGLAALKSEREAKT
                     AAEKRAAAAEARVKEFEDREKTEAQKQQEALAKAQQELAELTVAKTRAEVAAAKGVPV
                     ELLSGGTAEEIQAFAERLIAWRGEQPQEQQRFIVPAEGSQPNIPLNGDGLEAALKTAL
                     GIQ"
     gene            complement(16320..17096)
                     /locus_tag="VN041_02950"
     CDS             complement(16320..17096)
                     /locus_tag="VN041_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28034.1"
                     /translation="MATSAQIEEFRTAQASLVSLGQAQLRDVLSAFGESSDPAAVRDV
                     LLRYFPDFMTAFGDTAAVLGADFYDLVRDLPPSAGTARSVFAQPAKTKQSEGVVRWAV
                     GGLFATDADWAAFESLLLGAAQRLILQPSRETIDLLAQEDARSGRVAAVSWSRHVHPE
                     RARSHKSCDFCIMLAGRGPVYRSQEAAGSVIGRGSDRTGLDETGHRIAGGVGGGVRAR
                     GGQELAASFHDDCNCTTVPTFYEIQDRPGTRYPQVLVPVA"
     gene            complement(17252..18661)
                     /locus_tag="VN041_02955"
     CDS             complement(17252..18661)
                     /locus_tag="VN041_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HWU28035.1"
                     /translation="MPGIDLELDHTDASMLSALLKVWQDKRQRNAIRTVYFDGKAALK
                     DFGISLPPSMRSIEAALGWTAKGVHAVTDRSKFEGFVDSTGSDDPFGLEQIMWDNRFR
                     VEFPAAEVSSAVHGCSFLSVSHGDVQSGEADVLVLARSAESSAAIWDRRRRTLAGFLS
                     IVALDESKNPSEIIMYTPDKVITLTKTGTRWIVDVRRNPLRRVSVAPLVHKFELGRPL
                     GHSRITRASMYFTDAALRTIVRGEVSSEFYSAVEYWLFGADVSDFVGDDRWTAVMGRI
                     KAMDTDSNESADKPDLHRFNGASPEPHISQMRMWANLFADDQDLDVKFADTSNPSSAD
                     AIFAAKETLITTTRDANELWGHGAVESAQMAVMLRDGLDVVPGEMRALRAQFTDPAIV
                     SPTARADAYSKLSAADADFATSRVGRQYAGLSLEQITQLEAEQKRKSASDRIAQLVEA
                     ARGARQAVASQPASQPASQ"
     gene            complement(18661..20223)
                     /locus_tag="VN041_02960"
     CDS             complement(18661..20223)
                     /locus_tag="VN041_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28036.1"
                     /translation="MPSPSPTKTSTPTNSEPKLSDVAKHLVVPSGIKTTGYPAVEAQC
                     RKMGVEHDPWQKGLGRAILAKREGGLYAAGIGGVLISICRQVGKTFTIGTIIFALCIL
                     FPRIKVLWTAHHSATSDETFETLSALAKRRRIAPYIRQVRGGNGKQRIVFTNGSRIMF
                     GAREHGFGRGIPGVSIVVFDECQILKAKALSDMIPAANTVKNPLIIYMGTPPKPDDPA
                     EVFKARRKKALDVKKRRAAGEVVDFDTLWVEVGANQGDDVEDRRVLAKANPSFPHRTP
                     WEAILRLRENLTDPADWSREGLGIWDEELDGDGPLSKSWPMLAVDTPPTEGARAFGVA
                     FSADGMKVSLGGCIVEDERAHVELIDARQGPVDAALQPLADWFVQKVDGVPRWKRASA
                     IVLSGRAGALVLERLLIERKVPRRRIVVASSPQYFEACGMLLEQSTAAAREAKLGGPL
                     SLTHLSGEGQAQLDESAKKSIQEKRTRDGAWGWASPAGDETPVEAVSLALYGARTGRK
                     PRAAGERKAVIL"
     gene            complement(20177..20611)
                     /locus_tag="VN041_02965"
     CDS             complement(20177..20611)
                     /locus_tag="VN041_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWU28037.1"
                     /translation="MANAKAQLHAVGAPASAKSRGRRTYDEAFKRDAVEKARAAKSIS
                     QVATALGVSRPTLRAWMNAAAPPPMTLIEAARSGNRKAYLEALRDQLAEKIAAGMTPR
                     DLPPNVRLLNETMQQLEDLAAREREDSDAESVPDEDLDPDEL"
     gene            complement(20721..21092)
                     /locus_tag="VN041_02970"
     CDS             complement(20721..21092)
                     /locus_tag="VN041_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28038.1"
                     /translation="MGAPLHNANGHRRRQVVARVKAEEHDCALCDQHVDKTLNFITGG
                     HGKKCPSRDCTGCMPDPMRGEVDEDVPRSRGGSPYDRGNTHLMHRKCNQFKSDMTIAE
                     AREKLHGAKTTTRTVTASPIW"
     gene            complement(21097..21279)
                     /locus_tag="VN041_02975"
     CDS             complement(21097..21279)
                     /locus_tag="VN041_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28039.1"
                     /translation="MATKTAAQLTRGDIVQSEQGPSRVNAVTPDGNRVAVDLTTVDGG
                     IAWGDSFPAGHAFTLG"
     gene            complement(21452..21646)
                     /locus_tag="VN041_02980"
     CDS             complement(21452..21646)
                     /locus_tag="VN041_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28040.1"
                     /translation="MTTVIEVAEDACDRCGPSVRAYLYARMPSGRSLAYCGSCGTRFL
                     AGLLDQGATIIDQRHRIGEL"
     gene            complement(21792..22361)
                     /locus_tag="VN041_02985"
     CDS             complement(21792..22361)
                     /locus_tag="VN041_02985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWU28041.1"
                     /translation="MTDLNGHGTAEMRASGCACEWCDAWERATAEHHAREKAKRTTRL
                     LAMVTPQEDPDECWIWNHALTEEGYARLGWKGWSQLAHRVAYELLIGPIPEDMQIDHQ
                     CRTRSCINPRHLQAVTSQKNNENRAIIAETASGFRNVHWSEKRQRYCVVVRHERVSHW
                     GGSFVDKDDAIAAARDLRNRLHTNNLADA"
     gene            complement(22483..22815)
                     /locus_tag="VN041_02990"
     CDS             complement(22483..22815)
                     /locus_tag="VN041_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28042.1"
                     /translation="MGNVKLYPLTREWRFGKKATPKASGGQEPKKPRTKRPNPPKNTR
                     RCDRCGQLASYSTDDDTLGPHQWQSNGQWCRGGRAPSDSQRKAAAKAKRKRSVRASSG
                     GLPSLGQR"
     gene            complement(22835..>23129)
                     /locus_tag="VN041_02995"
     CDS             complement(22835..>23129)
                     /locus_tag="VN041_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28043.1"
                     /translation="NSHEDTAEWVRQYLFRRPVSPEPITEEQINAERARQIEKGHTPE
                     HDRQHGIAHVLRYVLHYGSRGEHLKAYAMVEAALEIAPPMSQNDDEQYAPNVG"
BASE COUNT         3706 a         8099 c         7270 g         4054 t
ORIGIN      
        1 gacggtcgcg gggccggagt ggtggcgggg ccaccgccgg tgcctgcgat ctgctgcgcg
       61 cgggcgatga tccagctgat gtccatcgag gggcccggac aggcggtcga cgtgcggcgg
      121 ccggcggggg tctcgttgtg accgaccacg cccggcacct cgacgacgtt cgcgttcgtg
      181 gttcccgtga cgcggcctct ctggatcggg aagccatacc gcttcgacgc ccacgccacc
      241 agatgcgcga tcgcttccat cgacgcgcgc gagatgcccc acgacggcgc accgctggtg
      301 ttctgcgtct cgcaggtcac cgcctgatgg tcgaccgcgc ctgtcgtcca cgcccggtag
      361 tggtccgggg ggacaatctc gtacacgctg ccgtcagcgt tgacctggta gttcggcgac
      421 acgcttcgat cgttcgaccc catgaaccgg gttcgcgccg atgccgccga ctgcgtcgag
      481 gccatgtgat ggacgatgaa cagccgcacc ttcccctgcc ggggagactt gttcgcggag
      541 acgcccgccc atgcggcgcc agggtattca gccacgatgg gctcctttca tgacgaagcc
      601 ccgtcggttg acggggctca ggttttcgaa ggtgggctag gcccgatagg gtccaggtgg
      661 ggacgacaca ccggaggaag cgtgcgcagc agcaggcgac agaggacgaa gacggtccgg
      721 aaggacattc aggggcttcg cgctctcgct gtgcttctcg tcttcgcgaa tcacatcgtc
      781 ggatggccga cgggcggctt cgccggcgtc gacgtcttct tcgtcatatc cgggttcctg
      841 atcacagggc tgctgctccg cgactacgag cgcacgggga cgatctcgcc gaggaagttc
      901 tacctcggca ggctcaagcg catcgtccct tcggccacgg tcgtgctgct tttcgtgatc
      961 ggattctcct gggccctgtt cacgaagacg cgtgccgtgt cggtcacatg ggacgcgttc
     1021 tgggccttca tcttcggctc caactggaac tacgcgatcg cgggcaccga ctacttcaac
     1081 caggacgcag cagtctcccc cctgcagcac tactggtctc tgtcggtcga agagcagttc
     1141 tacttcgtgt ggccctggct gctgctcgga ctgctcgtgg tcttcgcgcg gttcatcact
     1201 gtcgacggtc gtcgcgcacg gcagatcgcc gcgaccacga tcagcatcat cgtcatcgca
     1261 tcgttcgcgt gggccatgta cagcggcgcg acgcagccga cagtggcgta cttctccaca
     1321 ttcacccgtg cgtgggaact cggcatcggc gccctggtcg cgatcgccgc accgttgctc
     1381 agcaacatcc cccgcatcgc acggacggtg ctcgcgtacg tgggtctggt ggggatcatc
     1441 gcatcctgtg tgctgatccc cacgacggcc gcgtggcccg caccgtgggc gctgctcccc
     1501 gttctcgcta cggcgaccgt gatcgtctcc ggcatcggcg aggaagcccg cttcatccag
     1561 ccgctgacca accctgtttc ggtctacatc ggcgacatct cgtacagcct gtacctctgg
     1621 cacttcccgg cgatcatctt cggtcacgcg ctctacccgc aggccggatc gctcgcatac
     1681 ctcgggatcg ttctcgccgg cttcgccctc gcgaccgcgc agtactacgc gatcgagcgc
     1741 ccgatctgga agtcgccgtt cgggacgggt gcgcgttccg gggactggaa gcggtggtgg
     1801 aatgaccact ctcttcccgt ccaacgcggc ctggtcggtg gcatgatagc catgagcgcg
     1861 gccgtcatcg gcgtcgtctt cgtgcctacc ccggcgcccg tggattctcc ggcggctgcg
     1921 gcgacaccgc aaccaatctc gaccgccatt gacgagaccc cagctgttgc cgccgtgaac
     1981 cagtccgtcc gatctgctct gtcccttacg acctggccgg agctcaaccc cagcatcgac
     2041 gaggttgggc cgaactccaa ggtcgacgca tgggtcaatg acggttgcct tgcactcgag
     2101 aagggcgcag ggcgcgaccc gctgagcacg ttgaagcgct gcacctacgg tccggccgga
     2161 ggtgagcgga tcgccctcgt cggagactcg atcgcgatca gctggctgcc cgcgatccaa
     2221 gccgcatacc cgtctgctga gatccgcgtc atgacgatgc agcagtgccc gttcgcggcg
     2281 gtgtctgtga agaagggcga cggttcgtct ctcccagagt gtgatgcctt ccacgaggcg
     2341 gtcaatgctc agcttgccga gttgaagcct accctcacgg tgctctccca ggctgagaac
     2401 agtgccgcgc gaaccaccgg cgataccgac ctcacggccg gtatcgcgga ggccgtccag
     2461 tcagctaccg ccgtctcgga ccgcgtcgtg atccttccgc cgccgccgca gggccgcaac
     2521 atctcggact gctactcggc gcaaggcaca cccagcgcct gcaccgtcga caccaacgcc
     2581 gcgcaccggg ctgcgatcaa agccctgaag gatgcggtcg aggcgactcc ggaggtgcgc
     2641 gtgatcgaca cggacggact cttctgcctc gccgggcgct gcccggcaat cgctgacgga
     2701 accatcatcc gtgccgatgt tgcgcacctc acccagcagt actccgccaa gctcggtccg
     2761 gcacttgccg agttgctccg cccagaaggc tgagcggagc aacccgagta ggtcaggtca
     2821 gctcgaggat gtcgaagtcc tggacccaga tcttcccgga catggccgcg tttccgaacc
     2881 agacctcccc gagtagcgct ccaggaggcg ggttgagcac gagcatgacc gtgcgccatc
     2941 cgacggtgtc cgccgtcgtc gcttgcggaa tcagcgtggc cacgagctgg ccggtactga
     3001 tcgccgttgt cgcgctggta tctgcccacc aggagacgcg catatccatg ccgccggtac
     3061 cgatcagcag attctcccga gcgaaggaca ccgacactgc catctgccga cccgcccgac
     3121 agaggaagcg gcgagcgagg aaggtgcctg tccctgacgt cgtggcggaa gagaacagga
     3181 ccgcgcctgc gattccgtcg cgattggtct ttgcgctgtc gaacgagatc gcaagcggcg
     3241 tggtggatgt gctgtaagac gatcggaact gcgcctgatg tggctgcgcc gtcccggtcc
     3301 caaccgtcgg caacgcggca agagcgccgc cgagccactg cgatgcggac ccgatggcga
     3361 tctcctgggg aggactgaac ggggcgatct gcggtcgcat cttgatcagg ttcgcgctgg
     3421 caccccacgg gtgcgtcaag cccgactcat ccttcacaag gatggctccg gcactgttgg
     3481 agcggtcgat gatgtgaagc ccgtcaacgc tcaggctcac ggggttggtc gaccctccga
     3541 gcggagcgcc gatgcggaac agtacgtggc gtaccgagtc ggtccgggtg gagatgtaga
     3601 gctgccctcc cgtgaacttc gccacggtcg acgcgctccc cccggatgtc atcttcaccc
     3661 actgcggtcc gtactttgtt ccctcgaagt ggcagtcgtc ccagttgacg gtcccgccat
     3721 tcagatcggc aacctgcgca ccgccgtcga atgagcactg ggagaacttg atgtcgccag
     3781 cgttggtggg ggccgtgtac acgaacgtgt cgttgttgtt gttgaagatc ttgcagtgct
     3841 caaaatggaa gttctctccc atgttcaggc caccgaagga gaacgtgcgg cccatcgcgt
     3901 aacggaacga gcagttggag aacttccaca ggtatgcgtt gtccttcaga acgacctggt
     3961 tcttgaagcc ctggacgctg cagttctcga acctcacgtt gacggtgtcc gcggtggtga
     4021 ggtgggtgat cccgtgggtg ttcgtgaagt tgctcagcga cccgaggaag tgcaccccac
     4081 ggcagacgga ccacttcgac tgagcgatgt cgctgacgaa agccgtcggg tccaaggtga
     4141 acacggtgac gttgttggtc gagatcccgc tgaagtcgag gatcgtgttc gacccgccct
     4201 cgatgtcatg aaggcgatgc ttccatgtca gcagcgagtt gaccttcacc ttcccgcccg
     4261 gcagacgcaa cgtgacctgc agggtcgcgg ccgtggcgag agcactcacc attgccgcgc
     4321 cgtcgtcagt cgtgccgtca gcggtcacgc cgaagtccgt ggcgaagatc tcaccccgag
     4381 tcccaaccca ctgcgccgcc tccgtcgcag tccacgatgc cgctgcattg ccctcagtaa
     4441 gccttcggac gaggttgcct gctggcgtga tgacgacagc gcctgccgcg tagtggcggt
     4501 tcgcttgcca acgccccgca aacgtcgccg acatctgacc caggaactca gagccctggt
     4561 ctgcggctac gcctgccatg atcgcatcgt tcgtcgtacc aacggccgtc gcggccgctg
     4621 cggccgcgac ggcgtcatct gcggcgctgg tcgccacatc ccgagctgcc gtcgcatccg
     4681 cgcccgcctg gatcgccgct gccaccgtat ctgggtcaag cccagcggcg aggatagcgc
     4741 cgtacaccga cgtcagcttg gtcacgaacg gacccgactt gatcagcacc tgcggcgggt
     4801 tgccatcgac gcggaagtcc ggcaggacgc cgatgctcga agaccgcagt tccggaatcg
     4861 tcgcacctga ccccggttcc agcaccgtca gcggcgtgga cagactcgtg tcatcggtcg
     4921 cgtggaccgt gaacgcggca tccgcgacca ggcggtcctg agtcgggtcg tacgcgatcg
     4981 gggttccgaa gtagtcctgg aaagccatgg agagcgctcc tgcctatgcg ggcgtgacga
     5041 agacgccgcc cttgccgatg aggatctgag ccgggacgaa accgccggcc acgccgaccg
     5101 ctgtcgaagc gatcgggaat gccccgccaa cacccacacg ggcgccggac gggagggtcg
     5161 tcgcggagat gatctgagac cacgccccca cgccgaggct gttcacccca cgggagcgga
     5221 agtagacctt cgtcccaggg ggaaggtctg tgaagtcggt cgtccccgac gacgtcacga
     5281 tcggactgtt cccggtcgcg aacgtcggcg acgtcgacca ctgcgcttcc caccgcagga
     5341 acgtcccgcc gccgaggccc tgcgagctga acgagtaccg cgccgaggtc tgcgtgacgt
     5401 tcgtcacgcc cagcggcttc ggtgccggcg gggtcgtcgc gatctgcgaa agctggatcg
     5461 acccgtatgc cgtcgtctgc acgttgccgt aggacagctg catcccgagc ggatacgtcg
     5521 tcgacgtgcc cgaccagaag ccgctcccgt tcgcaggcac ccaccagtcg tacggcccct
     5581 gcgtccagct cgtgatgccc gacggcagga atggtgagcg tgggtagcgg gcgatctcgc
     5641 cgggcgctcc ctgcaccgac tggaatccgg agccgccgta ggacgagctc gatcccttcg
     5701 acatgcgcag gtacgcgcgc agcagccacc gcatgcccgc ggtatccgtc tggatgacgt
     5761 cgcagtcgag gtagaacgac gcgtacgggg atggcccctg gttcgaggtg atccggttcg
     5821 tcgccacagg gcaccacctc ctacggcttc ttcacccaga cgaggttgtc cgcgtacggc
     5881 ggctgggtgg accccacgta caccgagtcg ggcacccatg tctggttccc ggatgcgtcg
     5941 aacccgatca cccacgtgac cccgttgatc cagatgcggg tgccgatccg ggtgaggtag
     6001 tcccgcacca gtgcatggcg tgccgcgagc ccgccgtcgc cgtgccacac ccgcaggtcg
     6061 atgacctcct gcgcggccgt ctgcccggcg gagaaccgca ccagcgcgag cggctggtca
     6121 tcctccacac ccggggtcgt ggaccgcgcc gggatggatg ccgccgaccc gccctgaatc
     6181 agcaccagcg tggacgcctt cgcgccccac tgccggcgca gcgcgaccag atcccaccgg
     6241 ttccccgatg cgaccggcgc acccaccagc gactcgacag cgtcggactc gtcgaggatg
     6301 ccctgccctg ccgcggttcc tggctgcacc gacaggacac ggtcacccgt tccgggcgcg
     6361 acggcgaagg aatcctttcc gatgacgccg tactgggcgc ccatgtgcga ggtcagctgc
     6421 gaccattccg cgtagtcgac cgtcccctcg aaccctgtcg aagtgatcat cagcgcctcc
     6481 cagcgtctct cagcccccgt gcaaggcctg cgacctgcgc gcccaactgg gcgtcggggg
     6541 aatcctcgat cgaccccaga tgcggggtca cgaccacgcc ctgatcgggc gagtcctcca
     6601 cccgtgcgag ggtgatccgt tcgatcagat ccagcgggcc gaccttcacc cgcacccggt
     6661 cgccgggcag gaacgtcgtc ccgagccgga acttcaccga ctcctgcagc tgcatcgaca
     6721 cgcccaccgt cggcgcaccc tcggcgaggg cttcggctgc gtcgatcgag aagtccgcgc
     6781 cggcgtccgc catgcgcgag tccttgaacg tctcgatgat gtcgccccag tcggcctcgc
     6841 gtgccgtgtc gatctgctgg tacagctcac gggccgcacc ctcaccggca ccgccgacga
     6901 ccacacgggt cgcggatggg gcgtttcggg tccagtcgta gtcctcgagc gccccggtca
     6961 ggtccgacag cagacccggg acaagagcgg atgcgcgcac gtcgacgaca gggtcgggcg
     7021 cgtacccgat cgtcacgatc aggtcgtccg cgtccagggc ggggatcgtc ttctccgcga
     7081 gcgggtggaa ccggaactcc gccctcgccg cactcccccg ccccagtgac gcggcgaccg
     7141 tccacggcac gcccaggcga gagaagttcg ccgccagcgc cgccttgaac acgtcctcgg
     7201 aactgcccgt gtaccgggca tagtcgacgt cctgcgcggt gatcgccgcg gtcggcttcg
     7261 gccagccctg ccactccgtg agcttccgga agtcgccctc cacccgtgcc gtcaccgacc
     7321 cgaccgggcc gttcccgggg gtcgcggaca cgcggccgcg gaaccactcg atcccacgga
     7381 accagtacgc gcatcgggcg ccgtccgcag tcagcgcggg cagtgcatcg tggtcatcgt
     7441 cgagggtgag ctccgcccac ggcgtgctgt tcgccgggat ctccgccgtc gacaggcgcg
     7501 ggaccaccgt gcgccggaag gtgccggcct tgtcgtagac cacgaaccgg atctcgtcat
     7561 ccaccaggac cacctctcac atggcgcgga agtacagcgg ggtcagctcc gcggagatcc
     7621 cgcccgcgcc ggacgcggac acggtcagcg gggctgagcc cccggacggg accggcgcga
     7681 actcgagcag acccagcggc tcggtcgcgt ccaccccgtc cagcagcgcc gaaacgttcc
     7741 gcgggtcggt gtcgatgcgg agcacctggc cggcggcgag gctgaacggc accgtcgcgg
     7801 tcaccccgcc gacgccgagc accacgtccg tcaacggccc ctcgaccgtc cacacgagat
     7861 aggcgtccac gtccccgaag ttcgggatcg tggccgaggc gaacgtcgcc gccgggctga
     7921 tgtggaacga cggcgccagc gtcggcggga tgaagtcgac gccggtgggt gcggagaacg
     7981 gaccgcgacg gatcgtcttc ccctcccaga acggctgctc cggttccatc tgcaccggga
     8041 tgatcgccca cccttccatg tacgggtcga gggcgaactg gtgctgctcc tcaaagcgcc
     8101 cagtcagccg caggcgccgc gtctgcgtgc cagcggtcac ctcccacacg ccggctttcg
     8161 aggggtggat ggtggcgaag aaccgggagt acagcgagcg ccaggtctcg gaaccatcag
     8221 cccagatgca cacaggccag aagacgtccc gggacgccgc gcgccacccc cggctccgaa
     8281 agccggggac ggcgcgcgac gtcgaacgga acttgtccac cttcgggaag tgcaggccgg
     8341 tgacgccgct gttgtcgagg aagagccccg actcagggtc gttcagatcc cacacggacc
     8401 cgtcatgccc gatccaccgg tatccgagag ccgagagttc cgaccccacg ggcgcaactg
     8461 ttggcgggga cgcaagcacg agaggcatac gtcaccccac cttcacgaga gcgcccgaac
     8521 ggtacagcgc ggccctctgc tctcgacgca accgttcaat gagctcggct tcgtcacgcg
     8581 tgtagatgtc cccgttgatg gtcacacctc cggcgactcc agcccgcaac gcctgcgact
     8641 cctcaggagt caggaccgct tccggcttcc cggactggtt cacggccatg ccgccgtgcg
     8701 gcagccaccc accctggtcg tacagcttcg gcaccacacc gccgttcgcc atcgcgagat
     8761 ggacatggct gtagtgctgc gccttcacct tcggcgacca gcccgagaac ggcttcccgt
     8821 tcagcaactg cgcggcaccc gccggcgtgt agatcagctc cgacgcgttc gggaacaggg
     8881 aacggatcgc gttgaacgtc gccatcgacg ccgggacgac atcgatcgcc cggccccgca
     8941 tgtggtacga ccccttcgcg ccgttcgcgg ccgcattctg cgcggcgctg cggaaccccg
     9001 acgtcagccg ggcacccggg agcgcgttgt gcacgatgtt ccacatcgcc tgccagccca
     9061 tgcccggcac ccccttgccc gggccgcctt cgaacacctt cgccaggttc ttcacgaggt
     9121 tgatcggcat ctgaccgacc gtcttcatga agatgttccc gccgccaccc atcagcggcc
     9181 ggatgatccc gtcgacgatg tgcttctgga tcgccttcac ggggtcagtg aggaagtcac
     9241 cgaccgtgga ggccgcggac acgatgttgt cccacacgtc gccggcgaag ttcccgagct
     9301 ccccgaacac gccgccgttc ttgaacgcct gcccagtgcg ggccatgcgg ttgagcatgt
     9361 cgacacctgc aggtccaccg acggcgcgag tgaactcggg gcgcatgatc gcctcaccgc
     9421 cggacagcgc caggcgcccg ccggtcggcg agaagaactg gtgcacgtcc cggcccggcg
     9481 tatagccagg cagcacgcca ccgctggcga acttcaccag cggtgccttc gcgagcttca
     9541 tgtccttcag cccgagctcc gtcaccaggt cattccagaa cgaccgcaga ccgttgttcc
     9601 acaccgtgtc gagcacgaag ttgatcggca ccgcggcggc cttcttgatc ccgtcccagg
     9661 tcttctcgat cgacgacttc gcgatctcga aacccttcgc gatcagatcg attcccttct
     9721 tgaagggagt gaacacgttc gtgtcgatcc agttccacgc ggccttcatc gcgtcggaga
     9781 tcgcggtcca cacgggctgc accacgttca cgttcagcca cgtgaacacg tctccgagga
     9841 ccttcagtcc gatcccgatc gcgtcgagga ccggcttgac gaacaccgcg tacatgacct
     9901 gcagcgcgat cccgatcgca tcgatcaccg gcttgatgat cgtggaccac agccagccga
     9961 acgcggcacc gatcgcgtcc agcgctgggc cgatcgcgtt cttccacaac catccggcga
    10021 cggcacccca gaaccggaag tagttcacca ccaggtcgaa gttgaacttg atcacaccgg
    10081 cgacgaaccc gacgaccgcg gagatcccgt cgaagacggg cttcaggatc gtctcgtaca
    10141 gccacgtggc agcggcaccg atcccctcga acgtcggccg aatcacgttc tcgtacgcgg
    10201 cgacaaagaa acccgagatg ttcgcccacg cctcgcccaa gaaccgggtg aactcgcgcc
    10261 agatgtcctg gaaccatgtc gtctgcgtcg cgatccacac gatcgcaccg acgagcagag
    10321 agattcccat gatgatcgcg ccgagcgggt tcgccatcag tgtgacgttc agaatcgcct
    10381 gtaccgctgt ccagatcttc acgcccgtgt acacagctgt cactacggcc acgagcgtcc
    10441 cgatcgaaga cgcgaggatg ccaacgagat ccttgttctc cttgatccaa gtgaagaagg
    10501 tcccgatggc cgctccgact tccttcgact tcgcctcgaa cccagagaac acggtcgtca
    10561 gatctgcaac gccgttcttc atctccggga acaggccgcg cagaatgccc tcaccaattc
    10621 ggccgagcga cgccatcatg ttgtctacga ggccggacca tgtgccgccc atttcggatg
    10681 cgaccgtccc gctagccgcg gtcgccgcgg ccgcgaactg gtcgaagttg atcttcccct
    10741 ccgaagccat cttgaacacc ttgtccgagg tgacgccaag caccttccca agctcctgat
    10801 agatcgggat tccccggtcc gcgagctgcc cgatgacatc gttctggaca ccattcgcct
    10861 gagtcatcgc cttcgagaag atggaaccca tctcgccgag gtcggtgcca gccgctgcgg
    10921 ccgcgttcgc aactgtcgac aggaccccct caagatcctt gccaggcttc actcccgccg
    10981 cgacagcctg agccgcgact gtagccgcgt cgcccagacc gaacgcagtc cccttaaccg
    11041 acgcgagcgc gttggccatg atcgtgtcca ccgtcgcggc gtcgtgaccg agaccgcgca
    11101 gcttcgcaga cgccgtgtcg atcgcgtcaa ggcgcttgaa gcccttgaca agggccgcgc
    11161 cgagtcccgc ggctgccacg gctccgacag ccgccagccc caccttcgct atcggcgcga
    11221 acccgtccac gaggcgctgc cccatggact tccctgaccc cttcagcgct gcgctcacat
    11281 cggccccgcc gagcgtcttc gtgatgtcgt tcttcacgcc aggcatccgg gtcgtgatgc
    11341 tcacatatgc gttcgcgatc tcggcgctgg ttgccacgag acacctccaa gcgattgcgt
    11401 agctgtctgc tcgcggccac catggacacc atgagcatca tcgaggccgc gggccgcaac
    11461 gggcagatcc atttcgacgg gaagaccgtc accatccgtc gggaagggtt ctttgggcgt
    11521 gccggccacg gtcgaggcga gaagatgatc cccatccggc agatcgctgg tgtccagatg
    11581 aagccatctg gcgctctcac caacggcttc atccagctgg ccgttcccgg cgagatctcc
    11641 acgacgaagg cgctcaaggg cggccgcacc atggacgctg cgaacgatga gaatgccgtg
    11701 atcttcacca agaagcaaca gccgcagttc gaggctctgc gcgatgcgat ccgcgatgca
    11761 gtagccgccc tctagctgtt ccgttcctga cgtcgctgcc aagcgagtgc cttcgcggac
    11821 tcggctgcaa cttctgctgc aacctcaccg gcggcacgcg gaagcggaat cggctcaggc
    11881 tgcttgccct tctgaccgtt ctccgaccag aagatcatgc gcagtgtgtg ctccactgct
    11941 cggagcattc gagcttcatt cgtcacggcg gggtctccgc ccatctcgcg gcccagcgca
    12001 caccctgtcg gcagatgctc taccaaggcg gccagctcac gcggtgaccg tggcggctct
    12061 gtgcgaccgc ccgcgtcgat gagaagccga atgccgtact cagactgcag cgacgctcgc
    12121 agggcaccct cgtgagcgtt gagggtgccc gcgagcgtca ggagtttccc tggccttgcc
    12181 cgagtgcctc gaacagatcc atgacaaact gcccgccatc tgcgatgctc acgcgaccgt
    12241 tggcgccgcg cagcgcgttc agcacccgct tgtagtcgac gtcacccacg aggcgacgca
    12301 gcagcgccgg catacgtgaa gcgtcgtcgc tgtcctgcgc cgcgcgaatg tcgtcgagca
    12361 cctcgaagtc gtcgagtgcc tcgtgcggga cggtcacgac gatgccgcgg atcgcgacgc
    12421 gggatgcctt caccttgcgg gtcttcggct tcccctcgtc gtcgacttca cctgtggggg
    12481 tgtcgatctc gacctggtcc accttgggct tgtcgtcctt cggcttgcgg tcctgcggct
    12541 gcttcgcgcc cttcgggacg ctggtcgatt cgacctcgtt cgcatcagcc atgctcaggc
    12601 ccccgtctct tccaggtcgg tcgcgaggtg ggtgtactcg ccgatgatct ccccgatgaa
    12661 cgggagcgtc gcgatgtccg cgttgttcag cacccggtcg ccgttcggcg cgatctcgag
    12721 gcgcgggatg atgtaccgct ccttcacggt cgtctcgtcc gcgtcgtaga agtccagcac
    12781 cgcggcgcgg gccgtgatcc gctgcccggg gccgcgggtc gtcttccgga cgccggcggt
    12841 caccgtcgag gtcttctcga cgtaccgcag tcccatcgtc tgcgccttcg actcgagcgc
    12901 ggagaaggtg acggtggtgc cgggctccga gatgcgggtg cggacgacac cgttgccctg
    12961 gtggccgcgc ttcttctcga cggagccggt cagcgactcg gtcacgccgt cgtcggacag
    13021 ccatccgacg tcctcgaacg cggggtcgag caccccgtcg atgtccgtcg gcagggtcgt
    13081 gccgagaggt gccaggtgga tggcgtcgat gtcggacccg aagatccgcg ccagttcagc
    13141 attcacgccc atgagcgttc tccttcggtt tcgccgacat cagccggcag cggtggtcgc
    13201 ggcgggtgcc gcagcctggt ctcgacgcgc gcgcacgcgc atcacggcgg tgacggtgta
    13261 tcggtcaccg ttggtatcgg ggtccgggtc gaagtacggt ccggcctgga tggtcacgcc
    13321 gcgcacgagc ggcatcgctg tgtactcgtt cagcagcagg tgcttcgctt tcgcggcgtc
    13381 gttcgaggcg gtgacggaat cggctgcgaa gcaggtaacc gtgatctgca ccgtctccac
    13441 agcccggttg tcggtgccac ctccggtgcg ccacgctcgc gcgaaacgtg ccggacgcgg
    13501 attcgggacc ttggtcgaga cgggcacacc gagacgcgat ctcaggaacg cgccgaccat
    13561 cgcctccgcg tctgcgaact caacgtccgg catgaccgac cgccttcgtc aacaccttgt
    13621 cgcgcgcttc ctgccgtcgg cctttcgcgg acttcgcgcg cacgaacgcg cgtgctgtgt
    13681 atctgtgcgg gaccacgttc acttcgaact ccgggcccgc ctcccgctgg atgctgttgg
    13741 cggcagccac gacggccgcc acggctccgg gggaggtcat cacatggttg atgccacgca
    13801 gcttcagctg cactcgcacg tccgtcatga ttcagccctc cacccttcgg agcgtcgcga
    13861 cgttcccgat gcgtccgaga cgcggatgcc gccactcccg cgtgactccc tcgaccgtgt
    13921 aggcattccc tcgcgcgatc acgagatcac gggagccgaa gacgacatcg gtcggcatgt
    13981 acacggcggg ttcgacgatg acccggtctt gcccctcgcg aggttcggat gttgaccccg
    14041 ggtcgagcgc gtagatgccc ttcgtgaccg ggtcacccca ggactcgatc gggttcccgt
    14101 gcgcgtccac agccccggcc acgaacggca tccagatcac gtcctcgctg acctgcctca
    14161 cgacggcccc acgtagaacg gtgacgtcga cgggatcagg tcgatgctga acgcgccgcc
    14221 acgggcgccc tgcagcttcg caagctcatc gtccgtcagc cccagcccac cgggcacgtc
    14281 tccgccatag gtgacggagt ccgtgaacgg gccggtggtc gtgttcgcct gacggatgcc
    14341 ctccgggttt cggaacacgc gcatcaccat cgccacggtg acgtccttcg ccagctcgag
    14401 caggtccgtg cgcgcggtgg tctcggcggc ttcggcgtcg atccgtgtct ggatgtcggg
    14461 cacccggaac cggatctcac gctcggcctt gtcgatccac ttctgtatct tcccggtgtc
    14521 ggtgggtgca ccctcaccga tccacgcgcc ggtcacatcg gtgggtccgg tccagctcgc
    14581 catgatgcct cctcgtgtgg atgggtcagg gtgaggaagg acggcgaccg tagccgccgc
    14641 cctcccgggt cactcggtct tggccgaagc cttctccgcg gacttggttg cggtcttggc
    14701 cgaagccttc tccgccttcc attcgccgcc gagcatcttc gcctcgtctt ccgagacacg
    14761 gacgacggtg ccgcccgtgc tggtcagccg gacctgcttc ttttcagcca tcgcagatca
    14821 ccccattcag gcggcgtcga cgtactcgac gtatgcagcc gtgtcgttga ccaggaagcc
    14881 gtactcggcc tcggcaagga cggcgacgag gttgttctcg aacagcgaga cgagctcgcc
    14941 gttgatcgtc accgtcgcct cggtggagac cttgtaggag atgcctccaa cgacacccca
    15001 ggccgcctgc gaccagtcac cgccgaaccc gtacgtcttc gtggtggcgt cgtaaacgcc
    15061 ctcaccgatg aacgcgttgc gcccgaggag gcgtccctgg cggatcggac cggcgttctc
    15121 cacgagcggg ctgtcgatga agatcgggcg gcctgccgtg tcgactgcgc cgttcagctt
    15181 cggctcgaag cggtcatcga gcgcgaaccc ggtgaggcgc tttccgtcgt tgacgagcag
    15241 cgcgaggccg gcgttgatgt cgccgaagat gccaccgttc gcctgcgtag tcgtaccgag
    15301 ctcgacgctc ttggtcgtct gcgcgatgta cgtcgagaac ggcgtgttag tgccgtgcag
    15361 cgccgcagcg tcgaatgcct ccgcgaacgc gtcgccgacc tggttgcgga tcaggctcat
    15421 gtagttgccg gggttcgccc ggacaacctc agccgagacg accgcgatcg tcgcgatctt
    15481 cttcggatcc atgttcttca gcgccagagc gcccttcgac gcgggcttct gagcaccctc
    15541 agccacccag ttcgccgtga ggcgaccggt gacgacgggg atggacttgc cgttgccacc
    15601 gaggggaacc tcgggtgcca gcgactggac ggtcgagctg cgcgcggcac gctcgaagat
    15661 gggctgcgac tgctcgcggg tcaggaaccc actgaagtca ctcagcttgg ttgcggcggt
    15721 gatcgccatg tttcactcct tgagttgggg catcactgga tgccaagagc tgtcttcagc
    15781 gcggcctcaa gaccgtcgcc gttgagagga atgttgggct ggctgccctc ggcaggaacg
    15841 atgaatcgct gctgctcctg gggctgctcg ccccgccatg cgatgagacg ctccgcgaat
    15901 gcctggatct cctccgccgt accgcccgag agcagctcga cgggaacacc cttcgccgcg
    15961 gccacctcag cgcgggtctt ggcgactgtg agctcggcga gctcctgctg cgcctttgcc
    16021 agcgcctcct gctgcttctg cgcctcggtc ttctcgcggt cctcgaattc cttcacccgg
    16081 gcctcggcgg cagctgcacg cttctcagcg gcggtcttcg cctcacgctc tgacttcagg
    16141 gctgcgagtc cgggagttcc gagcggctcg tcgggcgtcg cgccctgggc cggtgcttcc
    16201 ggtgcggcag gtgccggtgt ggccggcgcg gtttcggtgg tcatgcgtcc tccatcggtt
    16261 tagcggcagt cgcgccgcgc gagcccgagc gccgtcgcgg cgtcgggtga gaatttgggt
    16321 caggcgaccg ggacgagcac ctgcgggtag cgagttcctg ggcggtcctg gatctcgtag
    16381 aacgtcggga ccgtcgtgca gttgcagtcg tcgtggaacg acgctgcgag ctcctgcccg
    16441 ccccgtgcgc gaacgccgcc accaacccca ccggctatgc ggtgtcccgt ttcgtcgagt
    16501 ccggtgcggt cagaccctcg accgatgacg gaccctgctg cttcctgcga tctgtagacc
    16561 ggcccgcgtc cggcgagcat gatgcagaag tcgcacgact tgtgcgaccg cgcccgctcc
    16621 gggtgcacgt ggcgggacca gctgaccgcc gcgacccgcc ctgatctggc gtcttcctga
    16681 gcgagcaggt cgatggtctc gcgagacggc tgcagtatca gccgttgagc ggcgcccagc
    16741 agcagcgact cgaatgcagc ccagtccgcg tcggtggcga acagcccgcc gacggcccac
    16801 cgcacgacac cctcggactg cttcgtcttc gcgggttgcg cgaacacgga ccgcgcagtg
    16861 cccgccgacg gcggcaggtc acgcaccaga tcgtagaagt ccgctccgag caccgccgcc
    16921 gtatcgccga acgccgtcat gaagtccggg aagtaccgca agagcacgtc acggaccgca
    16981 gccggatcag aggactcgcc gaacgctgac agcacgtcac gcaactgcgc ctgcccgagg
    17041 gagaccaacg acgcctgcgc agtacgaaac tcctcgatct gcgcagacgt cgccatgtca
    17101 ctcctcagac ttgatcgtga tcggctggcc tgggatgaat ttcaccccat cgagcccgga
    17161 ccgagcagcg gcgtcgtccg cctcaacgcc ggcacgcctg agcacgccga gcgcgtcggc
    17221 ttgcgccttc acgacctgcg ccgcctctag atcactggct ggctggctgg ctggctggct
    17281 ggctggctac agcctgtcgt gcgccgcgag ccgcctccac caactgtgcg atccgatccg
    17341 atgcgctctt ccgcttctgc tcggcttcca attgtgtgat ctgctccaac gacagcccgg
    17401 cgtactggcg gcccacgcgc gatgtcgcga agtccgcgtc agcggccgac agcttcgagt
    17461 aggcatccgc acgagccgtg ggagacacga tcgccgggtc cgtgaactgt gcacggagag
    17521 cacgcatctc cccaggaacg acgtcaagac cgtcacgaag catcacagcc atctgcgccg
    17581 actcgacagc gccatgaccc cagagttcgt tcgcatcccg ggtcgtagtg atcagcgtct
    17641 ccttagccgc gaagatcgca tcagcgctcg atgggttcga cgtgtccgca aacttcacgt
    17701 cgaggtcctg gtcgtccgcg aacaggttcg cccacatccg catctgcgag atgtgcggct
    17761 ccggggacgc cccgttgaac ctgtgcagat ctggcttgtc cgccgattcg ttcgagtcgg
    17821 tgtccatcgc cttgatccgg cccatgaccg ccgtccacct gtcgtccccg acgaagtcgg
    17881 agacgtcggc accgaagagc cagtactcga cagcgctata gaactcggac gagacctcgc
    17941 ctcggacaat cgtgcgcagc gcggcatccg tgaagtacat gctcgcgcgg gtgatccgcg
    18001 aatggcccag cggacggccg agctcgaact tgtgcaccaa cggcgccaca gacacccggc
    18061 gcagcggatt tcgccgcaca tcgacgatcc atcgtgtgcc cgtcttcgtc agagtgatga
    18121 ccttgtcggg tgtgtacatg atgatctcgg acgggttctt cgactcatcc agcgcgacga
    18181 tcgacaggaa tcctgcgagc gtccgccggc gacggtccca gatcgcggcc gacgactcag
    18241 ccgatctggc cagcacaaga acgtcagcct cgccggactg gacgtcaccg tgcgagacag
    18301 acaggaacga gcacccgtgc actgccgacg acacctccgc cgccgggaac tcgacccgga
    18361 atcggttgtc ccacatgatc tgctcaagcc cgaacgggtc atccgatcct gtcgaatcga
    18421 cgaacccctc gaacttcgaa cggtccgtga ccgcgtggac gcccttcgcc gtccaaccga
    18481 gcgccgcctc gatcgagcgc atcgacggag gtagcgagat cccgaagtct ttcagggccg
    18541 ccttcccatc gaagtacacc gtccggatcg cgttccgttg ccgcttgtcc tgccagacct
    18601 tcagcagtgc cgacagcatg gacgcgtctg tgtggtcgag ttcgaggtcg attccaggca
    18661 tcacaggatc accgccttcc gttcacccgc ggcccgcggc ttccgtccgg tccgtgcccc
    18721 gtacagggcc agcgacacgg cttctacggg ggtctcgtca ccggccggcg atgcccaccc
    18781 ccacgcacca tcacgagtgc gcttctcctg aatcgacttc ttcgccgact cgtccaactg
    18841 cgcctgacct tcaccggaga ggtgtgtcag cgacagcgga ccgccgagct tcgcctcacg
    18901 ggccgccgca gtcgactgct cgagcagcat gccgcatgcc tcgaagtact gcggcgacga
    18961 cgcaaccacg attcgccggc gcggcacctt tcgctcgatg agcagacgct ccagcaccag
    19021 cgcaccagca cgtccggaca ggacgatcgc cgaggcgcgc ttccaccgcg gcaccccgtc
    19081 gaccttctgc acgaaccagt ccgcgagcgg ctgcagcgcc gcatccaccg gcccctgccg
    19141 cgcatcgatg agctcgacgt gcgcccgctc gtcctcgacg atgcatccac cgagggacac
    19201 cttcatgcca tctgcagaga acgcgacgcc gaacgcgcgc gccccctccg tcggcggcgt
    19261 gtcgacggcg agcatcggcc acgacttcga cagcggccca tcgccgtcca gctcctcatc
    19321 ccagatcccc agtccctcac gggaccagtc ggccgggtcg gtcagattct cacgcagacg
    19381 gagaatcgcc tcccacggcg tgcggtgcgg gaacgacgga ttcgccttcg cgagcaccct
    19441 gcggtcctcg acgtcgtcgc cctggttcgc cccgacttcc acccacagcg tgtcgaagtc
    19501 gacgacctcg ccggccgcgc ggcgcttctt cacatcgagc gccttcttgc gacgcgcctt
    19561 gaacacctcg gccgggtcat ccggcttcgg tggcgttccc atgtagataa tcagcgggtt
    19621 cttcaccgtg ttcgctgccg ggatcatgtc cgacagcgcc ttcgccttca ggatctggca
    19681 ctcgtcgaac acgacaatgc tgacgccggg gatgccacgg ccgaacccgt gctcccgggc
    19741 gccgaacatg atccgagacc cgttcgtgaa gacgatgcgc tgcttgccgt tgccgccacg
    19801 cacctgccgg atgtacgggg cgatgcggcg acgcttcgcc agcgccgaca gcgtctcgaa
    19861 cgtctcgtcc gacgtcgccg agtggtgcgc ggtccacagc accttgatcc tggggaacag
    19921 gatgcacagc gcgaagatga tcgtcccgat cgtgaatgtc ttccccacct gccggcagat
    19981 ggagatcagc acaccgccga tgccggcggc gtacaggcca ccctcacgct tcgcgaggat
    20041 cgcgcgtccg aggcccttct gccaggggtc gtgctcgacc cccatcttcc ggcactgcgc
    20101 ctcgacggcg gggtacccgg tcgtctttat gcccgatggc accaccaggt gcttcgcgac
    20161 atccgacagc ttcggctcag agttcgtcgg ggtcgaggtc ttcgtcgggg acggactcgg
    20221 catcagaatc ctcccgctcc cgtgccgcaa gatcctcgag ctgctgcatc gtctcgttca
    20281 gcagtcgcac gttcggcggc agatccctcg gcgtcatacc cgccgcgatc ttctccgcga
    20341 gctgatcccg cagcgcctcg aggtacgcct tccggttccc cgatcgagcc gcctcgatca
    20401 gcgtcatcgg cggcggtgcg gccgcgttca tccacgcccg gagcgttggc cgcgacaccc
    20461 ccagagcagt tgccacctgg gaaatcgact tggcagcccg cgccttctcc acagcgtcgc
    20521 gtttgaacgc ctcgtcgtat gtgcgccgcc cgcgagattt cgcggaggca ggggccccga
    20581 ccgcgtgcag ctgcgccttc gcattcgcca tggtcgctca ccccctggaa agtggaaaat
    20641 ccccggggag agataggccc aatgccgcgg gtgggcctgt gggcccgggg agggggtggg
    20701 gtgcccaggg gggcccgcgg tcaccagatc ggggatgcgg tgacggttcg ggtcgtcgtc
    20761 ttggcgccgt ggagcttctc gcgtgcttcg gcgatggtca tgtcggactt gaactggttg
    20821 cacttgcggt gcatgagatg cgtgttgccc cggtcgtagg gtgagccgcc gcgtgagcgt
    20881 ggcacgtcct cgtcgacctc gcctcgcatc ggatcaggca tgcatccggt gcagtcccgt
    20941 gatgggcact tcttgccgtg cccgcccgtg atgaagttga gggtcttgtc cacatgctga
    21001 tcacagagtg cgcagtcgtg ctcttcggcc ttcacccgtg cgacgacctg gcgacgtcgg
    21061 tggccgttcg cgttgtgcag cggggcaccc atgcttctac ccgagggtga acgcgtgccc
    21121 ggccgggaag ctatctcccc acgcgatgcc accatccacg gtggtcaggt ccacggctac
    21181 ccggttcccg tcgggggtga ccgcgttgac tcgggatgga ccctgctcgg actggacgat
    21241 gtcaccacgg gtcagctgtg cggcagtctt ggtggccatg acgtctcctc tgtcgtgatc
    21301 tcttcgctct gatgccacac tcgctggcgt ggcttgcagt ccggatgcgt gcctggcggg
    21361 atgcgggtga acacccaccc gatgaacggc caccccacga cgtgccacac gatcatcacc
    21421 gtcacgccga gtgccatccc ggccgcgagc ttcacagctc cccgatgcgg tgacgctgat
    21481 cgatgatcgt cgcaccctgg tccagcaggc cggcgaggaa gcgggtgccg cagcttccgc
    21541 agtacgcgag cgagcggccc gacggcatcc gggcgtacag gtaggcgcgg accgatggac
    21601 cgcagcgatc gcacgcgtcc tcagcgacct cgatgaccgt ggtcatggct cgtccgtgag
    21661 ctcatcgatc ggttcgaccg ggtcacaatg caggacagca gcgaacagag acggacgcgg
    21721 aacaccacac cccgggcaag gccacgacat gaccgcctcc actctgaggg atacacggga
    21781 tgccggcgcg ctcaggcgtc ggcgaggttg ttcgtgtgga gccggtttcg caggtcgcgc
    21841 gccgccgcga tcgcgtcgtc cttgtcgacg aagctgccac cccagtggct cacccgctcg
    21901 tgccggacga cgacgcagta gcgttgccgc ttctcgctcc aatggacgtt gcggaacccg
    21961 gatgccgtct cggcgatgat cgcccggttc tcgttgttct tctggctcgt gaccgcctgc
    22021 aggtggcgcg ggttgatgca tgatcgagtc cggcattggt ggtcgatctg catgtcctcc
    22081 gggatcgggc cgatcagcag ctcgtaggcg acacgatgag cgagctgcga ccagcccttc
    22141 catccgaggc gcgcgtaacc ctcttccgtg agtgcgtggt tccagatcca gcactcgtcc
    22201 gggtcctcct gcggggtgac catcgcgagc agacgggtcg tcctcttcgc cttctcgcgg
    22261 gcgtgatgct cggcggtcgc ccgctcccag gcgtcgcacc attcacaggc acatcccgag
    22321 gcgcgcatct cggcggtgcc gtgaccattc agatcggtca tgggacacct cctggcatgc
    22381 agaaggcccc gggagaatcc tcctcggggc caactaacgc tcacgagcta tcgtacacga
    22441 aatcggcggc cacgtttccc tgatcagctc ggcgagtcgg ggtcaccgct gcccgagcga
    22501 gggcagtcct ccagagctcg cccggacgct gcgcttccgc ttggccttcg ccgccgcctt
    22561 gcgctgactg tcgctgggcg cccggccgcc gcggcaccac tgcccgttcg actgccactg
    22621 gtgcggaccg agcgtgtcgt cgtccgttga gtaggaggca agctggccac accgatcgca
    22681 gcgccgcgtg ttcttgggtg ggttcggccg cttcgtgcgc ggcttcttcg gctcctgacc
    22741 tccggatgcc ttcggcgtcg ccttcttccc gaaccgccac tcccgggtca gcggatagag
    22801 cttgacgttc cccatgggcg ctaccgtacc gggattaccc gacattcggg gcgtactgct
    22861 cgtcatcgtt ctgggacatc ggcggcgcga tctccagcgc tgcctctacc atcgcgtacg
    22921 ccttcaggtg ctcgccccgc gaaccgtagt gcagcacata tcggaggacg tgcgcgatgc
    22981 cgtgctgtcg gtcatgctcg ggcgtgtggc ccttctcgat ctgccgtgca cgctcggcat
    23041 tgatctgctc ctcggtgatc ggctccgggg atacggggcg acggaacagg tactgccgca
    23101 cccattcggc ggtgtcctca tgcgagttc
//
LOCUS       DATMSX010000095         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_58159,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000095 DATMSX010000000
VERSION     DATMSX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58159"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VN041_03000"
     CDS             <1..391
                     /locus_tag="VN041_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWU28044.1"
                     /translation="AELLLESAVRLASASGAAVRLVSLMPLDVPAGLNTGAITLLKDA
                     HVDQVLASARDRLSGETDVEVLQAGGDSIEDAVAQLDWLPGEIVLVGSSRLAQPRRLF
                     LGSTAAKMLRELPVPMIVVPRTRAEGA"
     gene            394..2004
                     /locus_tag="VN041_03005"
     CDS             394..2004
                     /locus_tag="VN041_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWU28045.1"
                     /translation="MSESTKSPLSTAASAAAPGDAVPGTADPVTGGISRKGLSVGTVG
                     VLGALVMGISCIAPAYTYTAGIGGAVGAVGFQVPAILLLGFIPMLLVAFGYRELNKAM
                     PDSGTSFTWATRAFGPYVGWMAGWGLIVATILVLSNLAGIAVDFLFLLISQISGNEAI
                     AEITRNPFVNVAVCLIFVALATWVSYRDVSTSQKLQYGLVAFQLAILVLFAVMAIVET
                     VQGGAFEAHMPEFAWFNPFEIKGGLSMIVMGLSASVFMFWGWDVTLTMNEEAKDPERT
                     PGRAAMLTVISIIAIYLLLTVSSLMYSGIGDKGLGLTNEEIGGNVFFALSGPVMGGLA
                     FLLSLAVLTSSASSLQSTFISPARTLLAMGHYGAVPKRFAGVSPRFFTPGFATIASAV
                     VAGGFYAVMRFLSEDALWDTILALGMMICFYYGITALSCVWYFRHQWFDSGKDFFFKF
                     ASPLVGGVILLVLFVITAIDSLAPDYGSIAIGADENGEGGIGIVFFIGVGLLVFGGVL
                     MLVQRFVSPDFFRGKTLSMDAPPSLRQH"
     gene            2029..3504
                     /locus_tag="VN041_03010"
     CDS             2029..3504
                     /locus_tag="VN041_03010"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWU28046.1"
                     /translation="MSTALAPNEQELLARVPGGLFIGGQWTDAEEGRTFDVRDPSTNQ
                     VIASIADATPADGIRALDAAVAAQDAWAATAPRTRSDILRRAWELVQEHKEDLALLMT
                     LEMGKPLAESRGEVAYGGEFLRWFSEEAVRISGRYGSNPEGTGRMIVSQRPVGPSFFI
                     TPWNFPFAMATRKIAPALAAGCTVVVKPAALTPLTTIFFTKLLEEAGLPAGVVNIVQT
                     SRSSALSAPIIADPRLRKLSFTGSTPVGQKLIAQAAQGVLRVSMELGGNAPFVVFEDA
                     DLDKAVDGAIAAKFRNIGQACTAANRFIVHEDVADEFARRVAERVNAMKIGRGTEDDV
                     VIGPLIDADAVAKAQELVGDAVQRGARLLAGGNAIEGDGTFYEPTVLADVVAGSDILR
                     EEIFGPVLAIARFSDEAEAVRLANDTEYGLVGYVFTEDLARGHRMIDALETGMMGLNV
                     GVISNAAAPFGGVKQSGIGREGGAEGIHEYLSTKYTMLPYS"
     gene            3524..4885
                     /locus_tag="VN041_03015"
     CDS             3524..4885
                     /locus_tag="VN041_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWU28047.1"
                     /translation="MIDYAVINPATGETLATYDTFTDAQVEDAIARAASAATTWAATS
                     PAERATVIRRIAELHRERKDELGAIIVREMGKPIAAAVGEVEFAADIIEFYADNIEKI
                     TADQPLDIVGDGTAVVRRAPLGALLGIMPWNFPAYQVARFAAPNLAIGNTIILKHAPQ
                     CPESAAAIEAIYRDAGLPDGGYVNVYATNEQAATIIADPRVHGVSVTGSERAGAAVAE
                     VAGRNLKKVALELGGSDPFIVLSTDDLDAVVQNAVDARLDNNGQSCNGAKRFIVVDGL
                     YEEFTSKFVASLAAVSAEDPTQEDTVLGPLSSLAAAERLQEQVDTAVSQGATLLTGGT
                     REGAFFAPTVLADVTPAMDVYREELFGPAAVVYRVADEDAAVALANDTSFGLGSYVFT
                     TDAEQAERVANGIEAGMVYVNLVLADSPELPFGGVKRSGTSRELGLLAADEFVNKKLI
                     RKG"
BASE COUNT          765 a         1825 c         1746 g          817 t
ORIGIN      
        1 tgcggagctg ctgctggaga gcgccgtgcg cctcgcctcg gcatccgggg ccgccgtgcg
       61 gctggtctcg ctgatgcccc tcgacgttcc tgcgggtctc aacaccggcg ccatcacgct
      121 gctgaaggac gcccacgtcg accaggtgct cgcctcggcc agggaccggc tctccggcga
      181 gaccgacgtc gaggtgctgc aggccggggg cgacagcatc gaggacgccg tcgcgcagct
      241 ggactggctg ccgggagaga tcgtgctggt cggctccagc cggctcgccc agccgcgccg
      301 gctgttcctg ggctcgaccg cggcgaagat gctccgcgag ctgcccgtcc ccatgatcgt
      361 ggtgccccgc acccgcgccg aaggagcgtg aacatgagcg agtcgacgaa gtctccgctc
      421 tccactgcgg catccgccgc cgcaccgggc gacgccgtgc ccggcaccgc ggacccggtg
      481 accggaggca tctcccgcaa gggactcagc gtcggaacgg tgggcgtcct gggcgccctg
      541 gtgatgggca tctcgtgcat cgccccggcg tacacctaca cggccggcat cggcggcgcg
      601 gtcggcgccg tcgggttcca ggtgcccgcg atcctgctgc tcgggttcat cccgatgctg
      661 ctcgtcgcct tcggctaccg cgagctcaac aaggcgatgc ccgactcggg cacgtccttc
      721 acctgggcca cgcgcgcctt cggcccgtac gtcggctgga tggcgggatg gggcctgatc
      781 gtcgcgacga tcctggtgct ctccaacctc gccggcatcg ccgtcgactt cctcttcctc
      841 ctgatctccc agatctccgg caacgaggcc atcgccgaga tcacccgcaa tccgttcgtg
      901 aacgtcgccg tgtgcctgat cttcgtcgcg ctcgccacct gggtgtcgta ccgcgacgtg
      961 tccacctcgc agaagctgca gtacggcctg gtggcgttcc agctcgcgat cctcgtgctc
     1021 ttcgccgtga tggcgatcgt cgagaccgtg cagggcggcg ccttcgaggc gcacatgccg
     1081 gaattcgcct ggttcaaccc gttcgagatc aagggcggcc tgtccatgat cgtgatgggc
     1141 ctgtcggcct cggtcttcat gttctggggg tgggatgtca ccctcacgat gaacgaggag
     1201 gcgaaggacc ccgagcgcac gccggggcgc gcggccatgc tcaccgtgat ctcgatcatc
     1261 gcgatctacc tcctgctcac ggtcagcagc ctgatgtact cggggatcgg cgacaaggga
     1321 ctgggactca cgaacgagga gatcggcggc aacgtgttct tcgcgctgtc gggtccggtc
     1381 atgggcggcc tggccttcct gctctcgctc gcggtgctca cctcgtcggc gtcctcgctg
     1441 cagtcgacgt tcatctcgcc ggctcgcacc ctgctggcga tgggccacta cggcgccgtg
     1501 ccgaagaggt tcgccggcgt cagcccgcgg ttcttcacgc ccgggttcgc gacgatcgcc
     1561 tcggccgtcg tcgcgggtgg cttctacgcc gtgatgcgct tcctcagcga ggacgcgctg
     1621 tgggacacga tcctcgcgct gggcatgatg atctgcttct actacggcat caccgcgctg
     1681 tcctgcgtgt ggtacttccg tcaccagtgg ttcgactccg gcaaggactt cttcttcaag
     1741 ttcgccagcc ctctggtcgg cggcgtcatc ctgctggtgc tgttcgtgat cacggcgatc
     1801 gacagtctcg cccccgacta cgggtcgatc gcgatcggcg ccgacgagaa cggcgagggc
     1861 ggcatcggca tcgtgttctt catcggcgtc ggcctgctcg tcttcggcgg ggtgctgatg
     1921 ctcgtgcaga gattcgtatc ccccgacttc ttccgcggca agacgcttag catggacgcg
     1981 ccgcccagcc ttcgccagca ctgatcctcc tcgagagaaa gagaaaccat gagcactgca
     2041 ctcgcaccga acgagcagga actcctcgcc cgcgtccccg gcggcctctt catcggcggc
     2101 cagtggacgg atgccgagga ggggcgcacc ttcgacgtgc gcgacccctc caccaaccag
     2161 gtgatcgcct cgatcgccga tgcgacgccc gccgacggca tccgcgctct ggatgccgcc
     2221 gtcgccgcgc aggacgcctg ggccgccacc gccccgcgca cccgcagcga catcctgcgc
     2281 cgcgcctggg aactggtgca ggagcacaag gaggatctcg cgctgctgat gaccctcgag
     2341 atgggcaagc cgctggccga gtcccgtggc gaggtcgcct acggcggcga gttcctgcgc
     2401 tggttcagcg aggaggccgt gcgcatcagc ggccgctacg gcagcaaccc cgagggcacc
     2461 ggtcgcatga tcgtgtcgca gcgccccgtg ggcccgtcgt tcttcatcac cccgtggaac
     2521 ttcccgttcg cgatggccac gcgcaagatc gccccggcgc tggcggccgg gtgcaccgtg
     2581 gtcgtcaagc ccgcggcgct cacgccgttg accacgatct tcttcacgaa gctgctggag
     2641 gaggcggggc tccccgccgg cgtcgtcaac atcgtccaga cctcgcgctc gagcgcgctg
     2701 tcggccccga tcatcgccga cccgcggctg cgcaagctgt cgttcaccgg ctcgacgccg
     2761 gtcgggcaga agctcatcgc gcaggccgcg cagggtgtgc tgcgggtgtc gatggagctc
     2821 ggcggcaacg cgccgttcgt cgtgttcgag gacgccgacc tggacaaggc cgtggacggc
     2881 gcgatcgcgg cgaagttccg caacatcggc caggcctgca ccgccgcgaa ccgcttcatc
     2941 gtgcacgagg acgtcgccga cgagttcgcg cgtcgcgtcg ccgagcgcgt gaacgcgatg
     3001 aagatcgggc gtggcaccga ggacgacgtg gtgatcggcc cgctgatcga tgcggatgcc
     3061 gtcgccaagg cgcaggagct ggtcggcgac gccgtacagc gcggcgcccg cctgctggcc
     3121 ggtggcaacg cgatcgaggg cgatggcacg ttctacgagc cgaccgttct cgccgacgtg
     3181 gtcgcgggca gcgacatcct ccgcgaggag atcttcggac cggtgctcgc gatcgcccgc
     3241 ttctcggacg aggccgaggc cgtgcgcctg gccaacgaca ccgagtacgg gctggtcggc
     3301 tacgtcttca ccgaggacct cgcccgcggg caccgcatga tcgatgcgct cgagaccggc
     3361 atgatgggcc tgaacgtggg agtgatctcc aacgccgcgg ctccgttcgg cggtgtcaag
     3421 cagtccggca tcggccgcga gggcggtgcc gagggcatcc acgagtacct gtccaccaag
     3481 tacacgatgc ttccctacag ctgaaccgac gagaggaacc gacatgatcg actacgccgt
     3541 catcaacccg gccaccggcg agaccctcgc cacctacgac accttcaccg acgcgcaggt
     3601 cgaggacgcg atcgcccgcg cggcatccgc agccacgacc tgggcggcga cctcgccggc
     3661 cgagcgcgcg accgtcatcc ggcgcatcgc cgagctgcac cgcgaacgca aggacgagct
     3721 cggcgcgatc atcgtgcgcg agatgggcaa gccgatcgcc gccgccgtgg gcgaggtgga
     3781 gttcgccgcc gacatcatcg agttctacgc cgacaacatc gagaagatca cggccgatca
     3841 gccgctggac atcgtgggcg acggcaccgc cgtggtgcgc cgcgcacccc tgggcgcgct
     3901 gctgggcatc atgccgtgga acttcccggc gtaccaggtg gcacgcttcg cggcccccaa
     3961 cctcgccatc ggcaacacga tcatcctcaa gcacgctccg cagtgcccgg agtcggcagc
     4021 cgcgatcgag gcgatctacc gcgacgcggg tctgccggac ggcgggtacg tgaacgtgta
     4081 cgccacgaac gagcaggcgg cgacgatcat cgccgacccg cgggtgcacg gcgtatcggt
     4141 gaccggctcc gagcgcgccg gcgccgccgt cgccgaggtg gcaggacgca acctcaagaa
     4201 ggtcgcgctg gagctcggcg gctccgaccc gttcatcgtg ctctccaccg atgacctcga
     4261 cgccgtggtg cagaacgccg tggatgcgcg actggacaac aacggccagt cctgcaacgg
     4321 cgccaagcgc ttcatcgtcg tcgacggcct gtacgaggag ttcacctcga agttcgtggc
     4381 gtcgctcgcc gcggtctcgg cggaggatcc cacgcaggag gacaccgtgc tgggcccgct
     4441 gtcgtcgctg gccgcggccg agcgcctgca ggagcaggtg gacaccgccg tctcccaggg
     4501 cgcgacgctg ctgaccggag gcacccgtga gggtgcgttc ttcgccccga ccgtgctcgc
     4561 cgatgtgacg cccgcgatgg acgtctaccg cgaggagctc ttcggccccg ccgccgtcgt
     4621 gtaccgcgtc gcggacgagg acgccgccgt ggcgctcgcg aacgacacct cgttcggcct
     4681 cggctcgtac gtgttcacca cggatgccga gcaggccgag cgcgtggcga acggcatcga
     4741 agcgggcatg gtctacgtga acctcgtgct cgccgacagc cccgaattgc ccttcggtgg
     4801 cgtgaagcgc agcggcacct cccgcgagct gggcctgctg gccgccgacg agttcgtgaa
     4861 caagaagctc atccgcaagg gctgagtcgc agctccggct gcgtcatccg gaacaggaga
     4921 ttcgtccgga acaggaggat tgccgcggca tccgtcctgc tctcggcgga tctcctgttc
     4981 tcggcaggag ccggatacgc ctgggcacag gggagcacga gcgggccgtc gaggccgcag
     5041 gtgtcacctg tgtgccgtac ggcgagtgag cgccgatgag cgccgggtcc gcgctgccgc
     5101 gcgactcagc gcgtgagcgc agggagcacg acggcggcga tgcccgcggc cac
//
LOCUS       DATMSX010000096         6312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_58217,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000096 DATMSX010000000
VERSION     DATMSX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58217"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1162
                     /locus_tag="VN041_03020"
     CDS             125..1162
                     /locus_tag="VN041_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HWU28048.1"
                     /translation="MTDRSAEQSVTAQHASDLRRARRMLVIIGLLGPVVITAVVVALI
                     IGWLPSLPDPAATHWGVGGVDRLGSPTMYLWLAIGVGLGLPVLIVAMVLAMGRTQWGG
                     ASRLLGGMALGLSGLSAVINGGSVALQRGLSEAAQVGPIWPVVVGGFCALLVLTAVGW
                     VLQPDVRPTPAKPLEPAHLASISAGERVVWMATASMPRVAMLIIGFIVVLVVGITAVM
                     VFEAHEAFWIPLLTLVIIGFAFATSASFRVRAGADGLTVRSQVGFPQVHVPLDEIVSV
                     RAVECHPFGEFGGFGWRVGMDGRTGIVLRTGPAIEVERRDKRPLVVTVDGAEVGAATL
                     RAYLDRRGESS"
     gene            1159..2214
                     /locus_tag="VN041_03025"
     CDS             1159..2214
                     /locus_tag="VN041_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HWU28049.1"
                     /translation="MTAASAGERRGGDGIRWVGLIIPLVILLLGAIVVVILIPHVPDP
                     AAVHWSGDGKPDGFASPWLNLLPVGIGVVLVILFAWLARALPRLQQTRPGPAALSQGP
                     APTASTARFLAAVNLGLAVMLTVISIASMGVQLGVADAADAPDISGWAAVGSACAVVA
                     TLVGWFAQPAASDGDHRAASVEPSASGRTEWSGSVVMARSGQVLLGIGVLITVALTAV
                     LVARASLTGSWGSAVLMIGVTALLLLAVGTSLVFRVRVTSDGLRVRSVVGLPSTRIRL
                     SDIAKVEVLDVDPLRDFGGWGWRIAVDGRRGVVLRAGRALQVTRISGRRFAVTVDGAD
                     DAAAVLEGLRQASSAAR"
     gene            2226..3206
                     /locus_tag="VN041_03030"
     CDS             2226..3206
                     /locus_tag="VN041_03030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWU28050.1"
                     /translation="MSETQNAEGPSERMPRIGLNAIIAYVVVAMGLAWLVALPLWLGN
                     GLATPGAGLLLPVMMYAPAAAVLIVLFVMRPIPKGQRLRFLGMWPLRPAKRVIWMSVI
                     AVFGAFAVVLLALAIASMLGWITLDLVNFSGYREALEANPLVAASGKALPPLGVLILT
                     QLVSMPFAAITINALAAFGEELGWRGFLVPALRRYGTWTALLVSGAIWGLWHAPVILL
                     GYDFGRTDITGVLLMTGGCIAWGVLFGYLRLRSGSVWPAVFAHGALNASAGLPLVLFA
                     AGTRPDPAFALALGISGWIACAVVAIILLLTGEFRRQPALAESRPRHPGA"
     gene            3279..3938
                     /locus_tag="VN041_03035"
     CDS             3279..3938
                     /locus_tag="VN041_03035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWU28051.1"
                     /translation="MDSTPAAWRAADDFLADTLVGHDPALEAALAAQRAAGMPEIEVA
                     PVAGKLLNLLVRISGAARVLEIGTLGGYSTIWMARGVGEQGRVVTIEAEAAHAAVARA
                     SIDAAGVGDRVDIRIGRGADVLPTLVGGFDLVFIDADKESNTLYLDWAARLGHPGTVV
                     IVDNIGRDGEIVNDTSPDSKVVGTRDGLRMLGEDPRFDATALQTVGIKGWDGFAIAVV
                     V"
     gene            4057..5337
                     /gene="eno"
                     /locus_tag="VN041_03040"
     CDS             4057..5337
                     /gene="eno"
                     /locus_tag="VN041_03040"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWU28052.1"
                     /translation="MALIEAVGAREILDSRGNPTVEVEVLLDDGIVQRAAVPSGASTG
                     AFEAYELRDGDKSRYGGKGVLKAVEAVIDELGPAIEGVEASEQRVIDEILIEVDGTDN
                     KSRVGANAILGVSLAVAKAAADSADLPLFRYLGGPNAHLLPVPLFNVINGGEHADNGI
                     DFQEYFLAPIGADTYSESLRWGVETYHVLKSELKAAGFNTGLGDEGGFAPDLPSNREG
                     LDFLLKAIEKAGFTPGKDIAVGLDVAATEFFADGVYRVEGKEWSPEQLIEYFEGLVRD
                     FPIVTIEDALAEDDWDNWKALTDAIGSKVQLVGDDLFVTNPERLRRGIDTGVANSLLV
                     KVNQIGTLSETLDAINLAHRSGYTTMLSHRSGETEDTTIADLAVAVNSGQIKTGAPAR
                     SERVAKYNQLLRIEEELGDAAVFAGRSAFPRFKA"
     gene            5382..5936
                     /locus_tag="VN041_03045"
     CDS             5382..5936
                     /locus_tag="VN041_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWU28053.1"
                     /translation="MARRPAPPSPSPAKHPAKDRTARQDRSQQRGRPVRGEGRRVDVR
                     EWAGGIRLSGFAVIMLSLVVLGTWVLVPSIGTYLDQRQKIAALEHSVQLTKDQIAALE
                     QERTRWDDPAYITTQARERLYYTKPGEVIYLVDNDLDPADVPPAQQHVSDKIQERPSD
                     WMPQLLRSLTSAGLAKSAVETSAG"
     gene            5989..>6312
                     /locus_tag="VN041_03050"
     CDS             5989..>6312
                     /locus_tag="VN041_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HWU28054.1"
                     /translation="MTTPPYETPTAAEIAVVSSQLGREARGVVGIAARCVCGNPTVVA
                     TAPRLADGTPFPTFYYLTHPAATAAMSTLEATHVMPELAALLEDDEDVAAAYLAAHHA
                     YLADRA"
BASE COUNT          864 a         2216 c         2237 g          995 t
ORIGIN      
        1 tcgtctcagc atccgccggg ccgctcgccg aactcgccga cgagatcgcg gctctcgtcc
       61 gccgcggcgc cgaactcgga ctcaccccgg ccacgctggc cgcgatcatc aaggagatga
      121 cgtcatgacc gaccgttccg ccgagcagtc cgtgaccgcg cagcacgcga gcgacctgcg
      181 gcgcgcgcgc cggatgctcg tcatcatcgg cctgctgggt cccgtcgtga tcacggccgt
      241 ggtcgtcgcg ctcatcatcg gctggctgcc gtcgctgccc gacccggccg ccacgcactg
      301 gggagtgggc ggcgtcgatc gcctcggctc gccgaccatg tatctgtggc tggcgatcgg
      361 cgtgggactg gggcttcccg tcctcatcgt cgccatggtg ctggcgatgg gccgcaccca
      421 gtggggcggt gcctcgcgcc tgctgggagg aatggcgctc gggctctcgg ggctgagcgc
      481 cgtcatcaac gggggatccg tcgccctgca gcggggcctg tcggaggccg cccaggtggg
      541 gccgatctgg cccgtcgtgg tcggcggctt ctgcgccctt ctggtgctga ccgccgtggg
      601 ctgggtgctg cagccggatg tgcgtccgac tccggcgaag ccgctcgagc ccgcgcatct
      661 cgcgtcgatc tcggcggggg agcgggtggt ctggatggcc acggcgtcga tgccgcgggt
      721 cgcgatgctg atcatcggat tcatcgtcgt gctcgtggtc ggcatcaccg cggtgatggt
      781 gttcgaggcg cacgaggcgt tctggatccc gctgctcacg ctcgtgatca tcggcttcgc
      841 gttcgcgacc tcggcgtcct tccgcgtgcg cgccggcgcc gacggtctca ccgtccgctc
      901 gcaggtgggg ttcccccagg tgcacgtccc gctcgacgag atcgtctcgg tgcgtgcggt
      961 ggagtgtcac cccttcggcg agttcggcgg cttcggctgg cgggtcggca tggatggccg
     1021 cacggggatc gtgctgcgca ccggccccgc catcgaggtg gagcggcgcg acaagcggcc
     1081 cctggtcgtc acggtcgacg gcgcggaggt gggcgccgcg accctgcggg cgtatctcga
     1141 ccggcggggg gagagctcat gacggcggcg tcggccggcg agcggcgcgg tggcgacggc
     1201 atccgctggg tggggctgat catcccgctg gtgatcctgc ttctcggggc gatcgtggtg
     1261 gtgatcctga tacctcacgt gccggaccct gcggctgtgc actggtcggg tgacgggaag
     1321 cccgacggct tcgcctcgcc ctggctcaat ctgcttccgg tcgggatcgg cgtggtgctc
     1381 gtcatcctgt tcgcctggct cgcccgggcg ctcccgcgtc tgcagcagac ccgccccggc
     1441 ccggcggcac tgtcgcaggg gccggccccg accgcctcca cggcgcgatt cctcgccgct
     1501 gtgaacctcg gacttgccgt catgctgacg gtgatatcca tcgcgagcat gggcgtccag
     1561 ctcggtgtgg cggatgccgc ggacgccccc gacatcagcg gctgggccgc tgtcggaagc
     1621 gcgtgcgcgg tcgtcgcgac cctcgtcggc tggttcgcgc agccggccgc atccgacggt
     1681 gaccaccgtg cggcatccgt cgagccgtcg gcctcggggc gcaccgaatg gtcgggctcc
     1741 gtggtgatgg cccggagcgg gcaggtcctg ctcggaatcg gcgtcctcat caccgtggcg
     1801 ctgaccgcgg tcctcgtcgc tcgcgcttcg ctgaccggca gctggggctc cgccgtgctg
     1861 atgatcggcg tgacggcgct gctgctgctc gccgtcggca ccagcctcgt gttccgcgtg
     1921 cgagtgacgt cggacgggct gcgcgtccgc tcggtcgtcg gactcccgag cacacgcatc
     1981 cgcctgtccg acatcgcgaa ggtcgaagtc ctcgatgtcg atccgctgcg cgacttcggg
     2041 ggctgggggt ggcggatcgc cgtcgacggc cgacgcggtg tcgtgctgcg ggcggggcgg
     2101 gcgcttcagg tcacccgcat cagcggacgc cggttcgccg tgaccgtcga cggagccgac
     2161 gatgcggcgg ccgtgctgga gggactgcgg caggcatcgt cggccgcgag gtgaggagtg
     2221 tggagatgag cgagacgcag aacgccgagg ggccgagcga gcggatgccg aggatcggcc
     2281 tgaacgcgat catcgcctac gtggtggtgg cgatgggcct cgcgtggctg gtggcgctgc
     2341 cgctgtggct ggggaacggg ctggcgacgc caggcgccgg cctgctgctc ccggtgatga
     2401 tgtacgcccc cgcggcggcc gtgctgatcg tcctgttcgt gatgcggccg attccgaagg
     2461 ggcagcggct caggttcctg gggatgtggc cgctgcgccc tgcgaaacgc gtgatctgga
     2521 tgagcgtcat cgccgtcttc ggtgcgttcg cagtggtgct cctggcactc gcgatcgcat
     2581 cgatgctcgg ctggatcacg ctcgacctgg tgaacttctc gggctacagg gaggccctcg
     2641 aggcgaaccc cctggtggcc gcctccggca aggcgctgcc gccgctgggc gtgctgatcc
     2701 tgacccagct ggtctccatg cccttcgccg cgatcacgat caacgcgctc gccgccttcg
     2761 gggaggagct cggctggcga ggtttcctcg tcccggcact gcggcgatac ggcacctgga
     2821 cggcgctgct ggtcagcggg gcgatctggg gcctgtggca cgcaccggtg atcctgctcg
     2881 gatacgactt cggccgtacc gacatcaccg gcgtgctgct gatgaccggc ggatgcatcg
     2941 cctggggcgt gctgttcggc tacctgcggc tgaggtccgg gtcggtgtgg ccggccgtgt
     3001 tcgcccatgg cgccctgaac gcctccgcgg gtctccctct cgtgctcttc gcggccggaa
     3061 cccgccccga tcccgcattc gcgctcgcgc tgggcatctc cggctggatc gcgtgcgccg
     3121 tggtggcgat catcctgctg ctgaccggag agttccgccg tcagcccgcg ctcgcggagt
     3181 cgcgtccacg gcatccggga gcctgatgcc gtcggaggac cggatcgtct gccgtgcgat
     3241 cccttgaccg cacgcggcct gcggggttgg ctgagcacat ggactccacc ccggcggcct
     3301 ggcgggccgc agacgacttc ctcgccgaca ccctcgtcgg gcacgatccc gccctcgagg
     3361 cggcactcgc cgcccagcgc gcggcgggca tgcccgagat cgaggtcgca ccggtcgccg
     3421 gcaagctgct gaacctgctg gtgcgcatct ccggtgccgc ccgggtcttg gagatcggca
     3481 cgctcggggg ctattcgacg atctggatgg cccgtggggt gggagagcag ggccgcgtcg
     3541 tcacgatcga ggccgaggcc gcgcacgccg ccgtcgcccg ggcgagcatc gacgcggcag
     3601 gcgtggggga ccgcgtcgac atccggatcg gtcgtggcgc ggacgtgctg ccgacgctgg
     3661 tcggcgggtt cgatctggtg ttcatcgacg cagacaagga gtcgaacacg ctctacctgg
     3721 actgggcggc caggctcggc cacccgggca ccgtggtcat cgtcgacaac atcggacgcg
     3781 acggggagat cgtgaacgac acgagtccgg attccaaggt cgtcggaacc agggacggac
     3841 tgcggatgct gggagaggac ccccgtttcg acgcgaccgc cctgcagacc gtgggcatca
     3901 agggctggga cggcttcgct atcgcggtcg tcgtctgagg aggcatccgc tcgggggaaa
     3961 caccctcggg gcccgcgacg tcgaatgact agactgaacc gcggatcgac gtcgctccac
     4021 accaccaccc ttccctccct gaaaaggagc acacccgtgg cactgatcga ggctgtaggc
     4081 gcacgcgaga tcctggactc gcgcggaaac ccgaccgtcg aggtggaggt gctcctcgat
     4141 gacggcatcg tgcagcgggc cgccgtcccc tccggcgcat ccaccggcgc cttcgaggcg
     4201 tacgaactgc gcgacgggga caagagccgt tacggcggca agggcgtgct gaaggccgtc
     4261 gaggcggtca tcgacgagct gggccccgcc atcgagggcg tcgaggccag cgagcagcgc
     4321 gtcatcgacg agatcctcat cgaggtcgac ggcaccgaca acaagagccg cgtgggcgcc
     4381 aacgccatcc tcggcgtcag cctggcggtc gcgaaggccg ccgccgactc ggccgacctg
     4441 ccgctgttcc gctacctggg cggcccgaac gcgcacctgc tgcccgtgcc gctcttcaac
     4501 gtcatcaacg gcggcgagca cgccgacaac ggaatcgact tccaggagta cttcctggcc
     4561 ccgatcggcg ccgacaccta ctccgagtcg ctgcgctggg gcgtggagac ctaccacgtg
     4621 ctcaagagcg agctgaaggc cgccggcttc aacaccggtc tcggcgacga gggcggcttc
     4681 gctcccgacc tgcccagcaa ccgcgagggc ctggacttcc tgctcaaggc catcgagaag
     4741 gccggcttca ccccgggcaa ggacatcgcc gtcggcctcg acgtcgccgc aaccgagttc
     4801 ttcgccgacg gcgtgtaccg cgtcgagggc aaggagtggt cgcccgagca gctcatcgag
     4861 tacttcgagg gcctcgtgcg cgacttcccg atcgtcacca tcgaggacgc gctcgccgag
     4921 gacgactggg acaactggaa ggcgctgacc gacgcgatcg gctcaaaggt gcagctggtc
     4981 ggcgacgacc tgttcgtgac caaccccgag cgcctgcgtc gcggcatcga caccggcgtg
     5041 gcgaactcgc tgctggtcaa ggtgaaccag atcggcacgc tgtccgagac ccttgacgcg
     5101 atcaacctcg ctcaccgctc cggttacacc acgatgctct cgcaccgctc gggcgagacc
     5161 gaggacacca cgatcgccga cctcgccgtc gccgtgaact ccggtcagat caagaccggt
     5221 gcgcccgcac gcagcgagcg cgtcgcgaag tacaatcagc ttctgcgcat cgaggaggag
     5281 ctgggagacg ccgcggtgtt cgcgggccgc tcggccttcc cgcgtttcaa ggcctgatca
     5341 gggatcctga ccgcagagat ccgacgaacg aagggggagc catggcgaga cgtccggctc
     5401 ccccttcgcc gtccccggcg aagcatccgg ccaaggaccg cacggccagg caggaccgct
     5461 cccagcagag gggccgcccc gttcgcggcg agggccgacg cgtcgacgtg cgcgagtggg
     5521 ccggcggcat ccgcctctcc ggcttcgccg tgatcatgct gtcgctcgtc gtcctcggca
     5581 cctgggtcct ggtgccgtcg atcgggacct acctcgacca gcgtcagaag atcgccgctc
     5641 tggagcactc cgtgcagctg accaaggatc agatcgccgc gctcgagcag gagcgcaccc
     5701 gctgggacga ccccgcctac atcaccacgc aggcgcggga gaggctgtac tacaccaagc
     5761 cgggcgaggt catctacctc gtcgacaacg atctcgaccc cgctgacgtc ccgcccgccc
     5821 agcagcacgt cagcgacaag atccaggagc gtccgtcgga ctggatgccg cagctgctgc
     5881 gcagcctcac ctccgccgga ttggccaaga gcgcggtcga gacgtccgcc ggctgaaccg
     5941 caccgcgcac tgagtcttca cacaggtcgc gacccgtacg ctggtggggt gacgaccccg
     6001 ccttacgaga cgccgaccgc cgctgagatc gccgtcgtgt ccagccagct cggacgcgag
     6061 gcccggggag tggtgggcat cgccgcacgc tgcgtgtgcg gcaacccgac cgtggtcgcc
     6121 acggcgccgc gcctggccga cggcacgccg ttcccgacgt tctactacct gacgcatccg
     6181 gcggccaccg ccgcgatgtc cacgctcgag gcgacccacg tgatgcccga gctcgccgcg
     6241 ctgctcgagg acgacgagga cgtcgcagcc gcgtacctcg cggcgcacca tgcctacctc
     6301 gccgaccgcg cc
//
LOCUS       DATMSX010000097        11619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_48775,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000097 DATMSX010000000
VERSION     DATMSX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48775"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VN041_03055"
     CDS             <1..497
                     /locus_tag="VN041_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HWU28055.1"
                     /translation="FVLTAEAAGASAPGELWVRGPQVMVGYLNRPDATAEMLDAEGWL
                     HTGDIATVTADGIFRIVDRLKELIKYKGYQVAPAVLEAVLLEHPQIADAAVIGANDAD
                     GQEVPKAYVVRQAGAGLSGEEVMEFVAAHVAPHEKVRMVEFIDVIPKSSSGKILRKDL
                     RARA"
     gene            complement(579..1247)
                     /gene="rplQ"
                     /locus_tag="VN041_03060"
     CDS             complement(579..1247)
                     /gene="rplQ"
                     /locus_tag="VN041_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWU28056.1"
                     /translation="MPKPTKGPRLGGGPAHERLLLANLAAALFTHKSIKTTETKAKRL
                     RPLAERLITLGKRGDLHARRRALTVLRSNKDAAHILFNEIAPLVAEREGGYTRITKVG
                     NRKGDNAPMAVIELVLEPVTPKKKAAAKPAAKAAPKAEKVEEAPAEETAAPAALVDGG
                     FGEDSAAPLEDGSAPEGFDIKGNKDSMKFHQPGGQWYDATVAEVWFRTAEAAEAAGFV
                     EAGK"
     gene            complement(1283..2272)
                     /locus_tag="VN041_03065"
     CDS             complement(1283..2272)
                     /locus_tag="VN041_03065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWU28057.1"
                     /translation="MLIAQRPTLTEEKISENRSRFVIEPLEPGFGYTIGNALRRSLLS
                     SIPGASVTTIRIDGVLHEFSTIPGVKEDVTEIILNIKQLVVSSERDEPITAYLRKTGA
                     GEVTAADISAPAGVEVHNPDLVIATLNDTARFELELTIERGRGYVSASQNRNEYAEAG
                     QIPVDSIYSPVLKVAYRVDATRAGERTDFDKLVLDVETKSAISPRDAVASAAKTLTEL
                     FGLARELNVEAEGIEIGPAPVEAVLSSELSMPIEDLDLSVRSYNCLKREGINTVSELV
                     ALSETQLMNIRNFGQKSVDEVRDKLVSLGLSLKDSVPGFDGAHFYSGNEDESF"
     gene            complement(2404..2802)
                     /gene="rpsK"
                     /locus_tag="VN041_03070"
     CDS             complement(2404..2802)
                     /gene="rpsK"
                     /locus_tag="VN041_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWU28058.1"
                     /translation="MAAPKAAARKPRRKEKKNIALGQAHIKSTFNNTIVSITDPSGAV
                     ISWASSGGVGFKGSRKSTPYAAGMAAESAARQAAEHGVKKVDVFVKGPGSGRETAIRS
                     LQAAGLEVGSIQDVTPQAHNGCRPPKRRRV"
     gene            complement(2833..3207)
                     /gene="rpsM"
                     /locus_tag="VN041_03075"
     CDS             complement(2833..3207)
                     /gene="rpsM"
                     /locus_tag="VN041_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWU28059.1"
                     /translation="MARLAGVDIPRDKRVVIALTYIYGIGRTRSVEILKATEIDESIR
                     VKDLSDDQLVALRDHIEGNYKVEGDLRREVAADIRRKVEIGSYEGLRHRRGLPVRGQR
                     TKTNARTRKGPKRTVAGKKKAR"
     gene            complement(3387..3503)
                     /gene="rpmJ"
                     /locus_tag="VN041_03080"
     CDS             complement(3387..3503)
                     /gene="rpmJ"
                     /locus_tag="VN041_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWU28060.1"
                     /translation="MKVNPSVKPMCDHCRVIRRHGRVMVICKSNPRHKQRQG"
     gene            complement(3569..3790)
                     /gene="infA"
                     /locus_tag="VN041_03085"
     CDS             complement(3569..3790)
                     /gene="infA"
                     /locus_tag="VN041_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWU28061.1"
                     /translation="MAKKDGVIEIEGVISEALPNAMFRVELTNGHKVLATISGKMRQN
                     YIRIIPEDRVVVELSPYDLTRGRIVYRYR"
     gene            complement(3958..4626)
                     /locus_tag="VN041_03090"
     CDS             complement(3958..4626)
                     /locus_tag="VN041_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWU28062.1"
                     /translation="MAQAQSKPNWFVIGISAAVVVVLIALGAVVVWMNNRATDAGPAP
                     SSSKSFNADTGAISFGDGKDTVDIFVDFQCPVCSSFEQQFGPQLEKAAESGKITLNYH
                     PIAILDRFSQGTEYSSRSAAAAVCVASEQPDKYLAYAKLLFANQPKENTPGLETSKLA
                     DLAKEAGADKVASCITKETYKKFGVAQASKHDIQGTPTVDINGKRLDITQQADLQKLI
                     ALIS"
     gene            complement(4716..5564)
                     /gene="map"
                     /locus_tag="VN041_03095"
     CDS             complement(4716..5564)
                     /gene="map"
                     /locus_tag="VN041_03095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWU28063.1"
                     /translation="MFRKSIYKTPAQLRSMIEPGLITAAALEAVRPLVRAGVSTAELD
                     AVADRLIADRGAESNFKLVRGYRHTTCISVNEQVVHGIPGDLVLQPGDIVSVDCGAQY
                     QGWNGDSAVTFVVPDPGQPELVARREELSRVTEGSMWAGIAAMASASHIGEIGSAIQD
                     YIEAQGPSAVSGETYGILREYVGHGIGRKMHESPSVFNYRTPDAGVEIRPGLVLAIEP
                     MVTAGGEETFVEDDDWTVSTIDGSDGSHWEHSVARHEGGIWVLTAPDGGAAGLAPFGV
                     VPVPIA"
     gene            complement(5582..6178)
                     /locus_tag="VN041_03100"
     CDS             complement(5582..6178)
                     /locus_tag="VN041_03100"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWU28064.1"
                     /translation="MTTSARLLIVGPQGSGKGTQGVRVAEALNIPVVSTGDIFRANIK
                     AGTELGQQVTAILEAGDLVPDELTSEIVRDRLSQEDAADGFLLDGYPRNTTQVAHLDA
                     FLSESDAALAAVILLDVPREESLTRLRLRAVEQGRSDDTDEAIAHRLDIYEHETAPII
                     SVYEQRGIVDRIDGVGSLDEITARISAALSARGIGQLV"
     gene            complement(6175..7497)
                     /gene="secY"
                     /locus_tag="VN041_03105"
     CDS             complement(6175..7497)
                     /gene="secY"
                     /locus_tag="VN041_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWU28065.1"
                     /translation="MFSAIARIFRTPDLRAKILFTIGIIALYRLGSNVPAPFVHFPNV
                     EQCLAASAGTEGLLGLVNLFSGGALLKLSIFALGVMPYITATIIVQLLRVVIPHFETL
                     HQEGQAGQTKLTQYTRYLTIALALLQSATLVTVTRSGQLFGQAGDAACQKLLTNDVWW
                     AQMLIIISLTAGTGLIMWMAELVTEKGIGNGMSVLIFTSIAATFPSSMWAIAEAKGFE
                     TFLLVLVVGLIVMTLVVFVEQSQRRVPVQYAKRMVGRRTYGGTNTYIPIKVNMAGVIP
                     IIFASSLLYLPMLIAQFNTPADGSEPAAWVTWIMTYLVSGDQPLYMLIYFLLIIAFTY
                     FYVAITFNPVEVADNMKKYGGFIPGIRAGRPTAEYLDYVITRITFPGSLYLGIVALIP
                     LVALATVQANQNFPFGGASILIIVGVGLETVKQIDAQLQQRHYEGLLR"
     gene            complement(7611..8201)
                     /gene="rplO"
                     /locus_tag="VN041_03110"
     CDS             complement(7611..8201)
                     /gene="rplO"
                     /locus_tag="VN041_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWU28066.1"
                     /translation="MAEKNEAVEEKAPKAAPKKAAAKPAAAKAAPAKAAAKKAPAKKD
                     AEAEVSRPAVLKVHHLRPVPGANTPKTRVGRGEGSKGKTAGRGTKGTKARNTVRVGFE
                     GGQMPLHMRTPKLRGFKNPFRVEYQVVNLAKLAELYPKGGDVTVSDLVAKGAVRKNEK
                     VKVLGDGDISVKLTVSVDKVSGSAEQKIVAAGGSVN"
     gene            complement(8201..8386)
                     /gene="rpmD"
                     /locus_tag="VN041_03115"
     CDS             complement(8201..8386)
                     /gene="rpmD"
                     /locus_tag="VN041_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWU28067.1"
                     /translation="MAERLKVTQIKSKVSEKQNQRDTLRSLGLKRIGDSVVRPDDAQT
                     RGYVRAVAHLVKVEEID"
     gene            complement(8386..9054)
                     /gene="rpsE"
                     /locus_tag="VN041_03120"
     CDS             complement(8386..9054)
                     /gene="rpsE"
                     /locus_tag="VN041_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWU28068.1"
                     /translation="MTTEQPAAEGQAQSEPQREQRDGRRGGRDRGQSRDRNSRDRGDN
                     QFLERVVTINRVSKVVKGGRRFSFTALVVVGDGNGLVGVGYGKAREVPLAISKGVEEA
                     KRNFFRVPRVGSTIPHPVQGEAAAGVVLLRPAAAGTGVIAGGPVRAVLECAGIHDVLS
                     KSLGSSNTINIVHATVEALQSLEEPRAVVARRGLEYDAVVPQIIIRNEAKAAAAAAAQ
                     KVGA"
     gene            complement(9075..9434)
                     /gene="rplR"
                     /locus_tag="VN041_03125"
     CDS             complement(9075..9434)
                     /gene="rplR"
                     /locus_tag="VN041_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWU28069.1"
                     /translation="MAVKSKSAARARRHLRLRKKIVGTDVRPRLVVNRSARHVFVQLV
                     DDSKGHTVASASTLETDLRSFDGDKTAKARKVGELLAERAKAAGFTEAVFDRGGNRYA
                     GRVAAIADGAREGGLAL"
     gene            complement(9437..9973)
                     /gene="rplF"
                     /locus_tag="VN041_03130"
     CDS             complement(9437..9973)
                     /gene="rplF"
                     /locus_tag="VN041_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWU28070.1"
                     /translation="MSRIGRLPIEIPAGVTVSVDGREVAVKGPKGELTLTVASPIQVE
                     VQENQVLVTRPDDERASRSLHGLTRTLISNDIIGVTQGYTKGLEVVGTGYRVQQKGNS
                     VEFALGFSHPVLIDPPAGITLTVEGTNKLTVSGISKQAVGEAAANIRKIRKPEPYKGK
                     GVRYAGEVVRRKAGKAGK"
     gene            complement(9980..10378)
                     /gene="rpsH"
                     /locus_tag="VN041_03135"
     CDS             complement(9980..10378)
                     /gene="rpsH"
                     /locus_tag="VN041_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWU28071.1"
                     /translation="MTMTDPVADMLTRLRNANSAHHDSVTMPSSKLKTNIAEILQKEG
                     YIAGFEVTDARVGSSLTLSLKYGPNRERSIAGIKRVSKPGLRVYAKSTELPKVLGGLG
                     VAILSTSSGLLTDRQAEQKGVGGEVLAYVW"
     gene            complement(10523..11113)
                     /gene="rplE"
                     /locus_tag="VN041_03140"
     CDS             complement(10523..11113)
                     /gene="rplE"
                     /locus_tag="VN041_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWU28072.1"
                     /translation="MAATEAAEAVKVQPRLKAKYNTEIKKALQEQFGYANVMQIPGLV
                     KVVVNTGVGEAARDSKVIDGAVEDLTRITGQKPIVTKARKSIAQFKLREGQAIGAHVT
                     LRGDRAWEFLDRLVNLSLPRIRDFRGLSGDQFDGNGNYTFGLQEQSVFHEIDQDKIDR
                     VRGFDITVVTTAKTDDEGRALLKALGFPFKSTDAAA"
     gene            complement(11113..11472)
                     /gene="rplX"
                     /locus_tag="VN041_03145"
     CDS             complement(11113..11472)
                     /gene="rplX"
                     /locus_tag="VN041_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWU28073.1"
                     /translation="MANIKKGDLVQVITGRKQDKGGDRGKQGKVLEVLVEQNRVVVEG
                     VNYVTKHTRVGQTQRGTKTGGIETVEAPIHISNVAVVDPSTKQPTRVGHRVEEQVKDG
                     VKRTVRVRYAKKSGKDL"
     gene            complement(11475..>11619)
                     /locus_tag="VN041_03150"
     CDS             complement(11475..>11619)
                     /locus_tag="VN041_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HWU28074.1"
                     /translation="YIKFDENAAVILKNDGEPRGTRIFGPVGRELRDKKFMKIVSLAP
                     EVI"
BASE COUNT         1797 a         3695 c         4016 g         2111 t
ORIGIN      
        1 tcttcgtgct gactgccgag gccgccggcg cgagcgcccc cggtgagctg tgggtgcgcg
       61 gcccgcaggt gatggtcggc tacctgaacc gcccggacgc gaccgccgag atgctggatg
      121 ccgagggctg gctgcacacc ggtgacatcg ccaccgtgac ggccgacggg atcttccgca
      181 tcgtcgaccg gctcaaggag ctcatcaagt acaagggcta tcaggtcgcc ccggccgtgc
      241 tcgaggctgt gctgctggag cacccgcaga tcgcggatgc cgcggtgatc ggcgcgaacg
      301 atgccgacgg ccaggaggtt ccgaaggcgt acgtggtccg tcaggcgggc gctggcctgt
      361 cgggcgagga ggtgatggag ttcgtcgcgg cgcacgtcgc cccgcacgag aaggtgcgca
      421 tggtggagtt catcgacgtc atcccgaagt ccagctccgg gaagatcctg cgcaaggacc
      481 tgcgcgccag ggcctgagcc cgccagcccg cggagagacg aagaagcccg ccatccccga
      541 ggggatggcg ggcttcttct gtgcagtgtc gctgcggctt acttgccggc ctcgacgaag
      601 cctgcggcct cagcggcctc agcggtgcgg aaccacacct ctgcgacggt cgcgtcgtac
      661 cactggccac cgggctggtg gaacttcatg gagtccttgt tgcccttgat gtcgaagccc
      721 tcgggagccg agccgtcctc gagaggagcg gcggagtcct caccgaagcc gccgtcgacg
      781 agagcggccg gagcggcggt ctcctcagcg ggagcctcct cgaccttctc ggccttcggg
      841 gcggccttgg cggccggctt cgcagcggcc ttcttcttcg gggtgacggg ctcgaggacg
      901 agctcgatca cggccatggg cgcgttgtcg cccttgcggt tgccgacctt ggtgatgcgg
      961 gtgtagccgc cctcacgctc ggcgacgagc ggcgcgatct cgttgaagag gatgtgcgcg
     1021 gcgtccttgt tggaacgcag cacggtcagc gcacgacggc gggcgtgcag gtcgccgcgc
     1081 ttgccgagcg tgatgaggcg ctcagccagc ggacgcaggc gcttcgcctt ggtctcggtc
     1141 gtcttgatcg acttgtgggt gaagagcgct gctgcgaggt tcgcgagaag aaggcgctcg
     1201 tgtgcggggc cgcctccgag gcggggaccc ttggtgggct tgggcataat cgtctaactc
     1261 ctggtcggaa agaggtcggg tatcagaagg actcgtcttc gttgccgctg tagaagtggg
     1321 cgccgtcgaa accgggcacc gaatccttca gcgacagacc gagcgagacg agcttgtcgc
     1381 gcacctcgtc gaccgacttc tgaccgaaat tgcggatgtt catcagctgc gtctcggaaa
     1441 gggcgaccag ctccgaaacg gtgttgatgc cctcgcgctt gaggcagttg tacgagcgca
     1501 ccgacagatc gaggtcctcg atcggcatgg agagctcgct ggagaggaca gcctcgaccg
     1561 gcgcggggcc gatctcgatg ccctcggcct cgacgttcag ctcgcgggcg agaccgaaca
     1621 gctcggtgag ggtcttggct gccgaagcga cggcgtcgcg ggggctgatc gccgacttcg
     1681 tctcgacgtc gaggacgagc ttgtcgaagt cggtgcgctc accggcacgc gtggcgtcga
     1741 cgcggtacgc gaccttcagg accggcgagt agatcgagtc gaccgggatc tgaccggcct
     1801 cggcgtactc gttgcggttc tgcgaggcgg acacgtagcc gcggccgcgc tcgatggtga
     1861 gctcgagctc gaagcgagcg gtgtcgttga gcgtcgcgat gaccaggtcg gggttgtgca
     1921 cctcgacacc cgccggagcg gagatgtcgg ccgcggtgac ctcgccggcg cccgtcttgc
     1981 gcaggtacgc ggtgatgggc tcgtcgcgct cgctggagac gaccagctgc ttgatgttga
     2041 ggatgatctc ggtgacgtcc tccttcacac cggggatcgt gctgaactcg tgcagcacgc
     2101 cgtcgatgcg aatggtggtg accgatgcgc cggggatcga cgagagcagg ctgcggcgca
     2161 gcgcgttgcc gatcgtgtat ccgaaaccgg gctcgagcgg ctcgatgacg aaccggctgc
     2221 ggttctcgga gatcttttcc tcggtgagag tgggacgctg tgcgatgagc actatgtgtt
     2281 cctttcgatc acatgcccgc tatatgacat gtgttttggt gaggtcttga gttgtggaaa
     2341 tctgcgggcg actgtgccct gcggggcccg gaagggcccc agcagggcag cagtcatgcg
     2401 agatcagacg cggcgacgct tcggcggacg gcagccgttg tgcgcctgcg gggtgacgtc
     2461 ctggatggac cccacctcga ggccggcggc ctgcagcgag cggatcgcgg tctcgcggcc
     2521 cgaacccgga cccttcacga agacgtcgac cttcttgacg ccgtgctccg cagcctggcg
     2581 agcagcggac tccgctgcca tgcctgcggc gtacggggtc gacttgcggg agcccttgaa
     2641 gcccacgcca cccgacgagg cccagctgat gacagcgccg gacgggtcgg tgatcgagac
     2701 gatcgtgttg ttgaacgtcg acttgatgtg ggcctggccc agcgcgatgt tcttcttctc
     2761 cttgcggcgc ggcttgcgcg cggcggcctt gggtgcagcc atgaaagtgt tctcctaatc
     2821 cctggccgcc gcttagcggg ccttcttctt gccggcgacg gtgcgcttcg ggcccttgcg
     2881 ggtacgggcg ttggtcttgg tgcgctgacc gcggaccggg aggccacgac ggtggcggag
     2941 gccctcgtag gagccgatct cgaccttgcg gcggatgtct gcggcgacct cgcggcgcag
     3001 gtcaccctcc accttgtagt tgccttcgat gtggtcgcgg agggccacga gctggtcgtc
     3061 gctgaggtcc ttgacgcgga tcgactcgtc gatctcggtc gccttgagga tctcgacgga
     3121 gcgggtacgg ccgatgccgt agatgtaggt gagggcgatc accacgcgct tgtcgcgcgg
     3181 gatgtcaacg ccggcaagac gtgccatgcg gttctcctgg agttttgtgg aggtgtggag
     3241 cagcatccgt tcccgggcct ccgtcccggg gtgtccgcct ccgtcgcccc gcaggggcga
     3301 gccgaggcat ccgatgctgc ctgttgttcg atattgagtt gttcagctgc cgggccggat
     3361 gccgaggcat ccgatccgac ccctgctcag ccctggcgct gcttgtggcg cgggttgctc
     3421 ttgcagatca ccatgacgcg accgtgacga cggatcacgc ggcagtggtc gcacatgggc
     3481 ttgacgctgg ggttgacctt catgatgttt cctgttcgct gtcttcaccg tgcgggcgcg
     3541 ggcgcccgcg gtgttacttc tcgaccgatc agcggtagcg gtagacgatg cggccacggg
     3601 tcaggtcgta gggcgagagc tccacgacca cgcggtcctc ggggatgatg cggatgtagt
     3661 tctgccgcat ctttccggag atcgtcgcca gaaccttatg tccgttggtg agctcaacgc
     3721 ggaacatcgc gttgggcaga gcctcggata tgacgccctc gatctcgatg acaccgtctt
     3781 tcttagccat agcctcgctg acgcttctgc agatcggtcg atctgcggtg gatgggtgat
     3841 ggttggtggg cctcatccgg acacgccgga acaaggcgca aggcaccaag gatctatact
     3901 aaccgacacg cccgcctccg gcaaatgcag gggcgggcgt gcgggatgtg cggacgctca
     3961 gctgatcagt gcgatgagct tctgcaggtc ggcctgctgc gtgatgtcca ggcgcttgcc
     4021 gttgatgtcg accgtgggcg tgccctggat gtcgtgcttc gacgcctgcg cgacgccgaa
     4081 cttcttgtac gtctccttgg tgatgcagga ggccaccttg tcggcgcccg cctccttcgc
     4141 gaggtcggcg agcttgctgg tctccaggcc gggagtgttc tccttaggct ggttcgcgaa
     4201 gagcagcttg gcgtatgcga ggtacttgtc cggctgctcg gacgcgacgc acaccgccgc
     4261 agctgcggag cgggacgagt actccgtgcc ctgcgaaaag cgatccagga tcgcgatggg
     4321 gtggtagttg agcgtgatct tgccgctctc agccgccttc tccagctgcg ggccgaactg
     4381 ctgctcgaag ctgctgcaga ccgggcactg gaagtcgacg aagatgtcga ccgtgtcctt
     4441 gccgtctccg aacgagatcg cgccggtgtc cgcgttgaag gacttcgagc tggacggcgc
     4501 gggcccggca tccgtcgcac ggttgttcat ccacaccacg accgcgccga gtgcgatcag
     4561 cacgacgacg acagcggctg agatgccgat gacgaaccag ttgggcttgc tctgtgcctg
     4621 ggccatgaca gtccgatctt tctgcagaga agttgacacc ctattgtgcc gtgcgcacac
     4681 tatgccccgc ctgtgacgag gcttcgctcc cctgcctagg cgatgggcac ggggacgacc
     4741 ccgaacggag cgaggcctgc ggcgccaccg tcgggtgccg tcagcaccca gatgccgccc
     4801 tcgtgcctgg cgacgctgtg ctcccagtgc gagccgtcgg agccgtcgat ggtggagacc
     4861 gtccagtcgt cgtcctcgac gaacgtctcc tcgccgcccg cggtgaccat cggctcgatg
     4921 gccagcacga gacccggacg gatctcaact ccggcatccg gcgtgcggta gttgaacacg
     4981 ctcggggatt cgtgcatctt gcgaccgatg ccgtggccga cgtactcccg caggatgccg
     5041 taggtctccc ccgacacggc agaaggaccc tgcgcctcga tgtaatcctg gatggcggaa
     5101 ccgatctcgc cgatgtggga ggcggatgcc atggccgcga taccggccca catggatccc
     5161 tcggtgactc gggagagctc ctcccggcgc gcgacgagct ccggctgacc gggatcgggc
     5221 accacgaacg tcacagcgct gtcgccgttc cacccctggt actgcgcacc gcagtcgacg
     5281 gagacgatgt cgcccggctg cagcaccagg tcacccggga tgccgtggac gacctgttcg
     5341 ttcaccgaga tgcaggtcgt gtgccggtag ccccgcacca gcttgaagtt cgactcagcg
     5401 ccgcggtcgg cgatcaggcg gtcggcgacc gcatccagct cggccgtgct gacgcccgcg
     5461 cgcaccagtg gacgcacggc ctccagtgcg gcggccgtga tcaggccggg ctcgatcatc
     5521 gagcgcagct gcgccggggt cttgtagatc gacttgcgga acatcgtcat gcggacgctg
     5581 ctcagacgag ctggccgatg ccgcgggcgc tgagcgccgc ggagatccgc gcggtgatct
     5641 cgtcgagcga gcccacgccg tcgatgcgat cgacgatgcc gcgctgctcg tacaccgaga
     5701 tgatcggagc cgtctcgtgc tcgtagatgt ccagacggtg cgcgatcgcc tcgtcggtgt
     5761 catccgagcg gccctgctcc accgcacgca gacgcagtcg ggtgaggctc tcctcgcgcg
     5821 ggacgtccag caggatgacg gcggcgagtg ccgcatccga ctccgacagg aacgcgtcca
     5881 ggtgagccac ctgcgtcgtg ttgcgggggt agccgtcgag caggaacccg tcggcggcgt
     5941 cctcctgaga caggcggtcg cgcacgatct cgctggtcag ctcgtccggc accagatcgc
     6001 cggcctcgag gatcgcggtg acctgctgtc cgagctccgt ccccgccttg atgttggcgc
     6061 ggaagatgtc gcccgtggac accaccggga tgttcagcgc ctcggcgacg cggacaccct
     6121 gcgtgccctt gcccgagccc tgcgggccga cgatcaggag tcgtgcggat gtcgtcatcg
     6181 gaggagccct tcgtaatggc gctgctgcag ctgcgcgtcg atctgcttca cggtctccag
     6241 tccgacgccc acgatgatca ggatagatgc gccgccgaac gggaagttct ggttcgcctg
     6301 caccgtggcc agcgcgacca gcgggatcag cgcgacgatg ccgaggtaca gcgagccggg
     6361 gaacgtgatg cgggtgatca cgtagtccag gtactcagcc gtgggacggc cggcgcggat
     6421 gccggggatg aatccgccgt acttcttcat gttgtcggcg acctcgaccg ggttgaaggt
     6481 gatggccacg tagaagtagg tgaaggcgat gatcagcagg aagtagatca gcatgtacag
     6541 cggctggtcg cccgagacga ggtacgtcat gatccaggtc acccacgcgg cgggctcgct
     6601 gccgtcggcc ggggtgttga actgcgcgat cagcatcggc aggtacagca gcgacgaggc
     6661 gaagatgatc gggatcacgc ccgccatgtt caccttgatc gggatgtacg tgttcgtgcc
     6721 gccgtaggtg cggcggccca ccatccgctt ggcgtactgc accgggacgc gacgctgcga
     6781 ctgctcgacg aagacgacca gcgtcatcac gatcagaccg accacgagca cgagcaggaa
     6841 ggtctcgaag cccttggcct cggcgatggc ccacatcgag ctcgggaagg tcgcggcgat
     6901 cgaggtgaag atcagcacgg acatgccgtt gccgatgccc ttctcggtga ccagctcggc
     6961 catccacatg atcaggccgg tgcctgcggt cagggagatg atgatgagca tctgcgccca
     7021 ccacacgtcg ttcgtgagca gcttctggca ggccgcgtcg cccgcctggc cgaagagctg
     7081 tccgctgcgg gtcacggtca ccagcgtcgc ggactgcagc agcgcgagcg cgatcgtcag
     7141 gtaccgggtg tactgggtga gcttggtctg gcccgcctga ccctcctggt ggagggtctc
     7201 gaagtgcggg atgaccacgc gcagcagctg cacgatgatc gtcgcggtga tgtagggcat
     7261 gacgcccagc gcgaagatcg agagcttcag cagagcgccg ccggagaaga ggttgaccag
     7321 accgaggagt ccctcggtgc cggcgctggc ggcgaggcac tgctcgacgt tcgggaagtg
     7381 aacgaacgga gccggcacat tcgagcccag gcggtacagg gcgatgatgc cgatggtgaa
     7441 aaggatcttc gcacgcagat cgggcgtgcg gaagattcgc gcgatggcgc taaacaaggg
     7501 cgttcctcct gaagggattg ccgaacgtcg aaggacggcc gaaagtccag ggtaacgcac
     7561 aggaggggcc ggagaatctc cgacccctcc cagcgttcac agctgaatgc tcagttgacc
     7621 gaaccgccgg cggcgacgat cttctgctcg gcgctgccgg acaccttgtc gaccgacacg
     7681 gtgagcttga ccgagatgtc gccgtccccg aggaccttga ccttctcgtt cttgcgcacg
     7741 gcacccttgg cgaccaggtc gctgacggtg acgtcgccac ccttcgggta cagctccgcg
     7801 agcttggcca ggttcacgac ctggtactcg acgcggaacg ggttcttgaa cccgcgcagc
     7861 ttcggggtgc gcatgtgcag cggcatctgc ccaccctcga agccgacgcg aacggtgttg
     7921 cgggccttcg tgcccttggt gccacgaccg gcggtcttgc ccttggagcc ctcaccacgg
     7981 cccacacggg tcttcggggt gttggcgccg gggaccggac gcaggtggtg gaccttcagg
     8041 acggcgggac gcgagacctc ggcctcggcg tccttcttgg ccggtgcctt cttggcggcg
     8101 gccttggccg gagcggcctt ggcagcagcc ggcttggcgg cggccttctt cggagcggcc
     8161 ttgggcgcct tctcctcgac ggcttcgttc ttctcagcca ttagtcgatc tcctcaacct
     8221 tgacgaggtg agcgacggcg cggacgtaac cgcgggtctg ggcgtcgtcg ggacggacga
     8281 ccgagtcgcc gatccgcttg agacccagcg aacgcagcgt gtcgcgctgg ttctgcttct
     8341 cgctcacctt ggacttgatc tgcgtgacct tgaggcgctc ggccatcagg cacctacctt
     8401 ctgcgcggcg gcggcagcgg cggccttcgc ctcgttgcgg atgatgatct gcggcacgac
     8461 ggcgtcgtac tcgagaccac ggcgagccac gacggcacgg ggctcctcga gcgactgcag
     8521 agcctcgacg gtcgcgtgca cgatgttgat cgtgttcgac gaaccgaggg acttcgacag
     8581 cacgtcgtgg ataccggcgc actcgagcac ggcgcggacc ggaccaccgg cgatgacacc
     8641 ggtaccggca gcggccggac gcagcagcac cacaccggca gcggcctcac cctgcacggg
     8701 gtgcgggatg gtcgagccga cgcgcgggac gcggaagaag ttgcgcttgg cctcttcgac
     8761 acccttcgag atcgccaggg ggacctcgcg ggccttgccg tagccgacgc ccaccagacc
     8821 gttgccgtca ccgacgacca cgagagcggt gaagctgaag cgacgaccac ccttcacgac
     8881 cttcgagacg cggttgatgg tgacgacgcg ctccaggaac tggttgtccc cacggtcgcg
     8941 cgagttgcgg tcgcggctct ggccgcggtc acggcctccg cgacggccgt cgcgctgctc
     9001 gcgctgcggc tcgctctggg cctggccctc ggcagcaggc tgctcggtgg tcacttcgtt
     9061 ctccttgttg tcactcacag tgccagcccc ccttcacggg cgccatcggc gatggccgcg
     9121 acacgaccgg cgtagcggtt gccgccacgg tcgaacacag cctcggtgaa gccggctgcc
     9181 ttggcgcgct cggcgagcag ctcgcccacc ttgcgggcct tggcggtctt gtcaccgtcg
     9241 aaggagcgca ggtcggtctc gagcgtggaa gcggatgcca cggtgtggcc cttgctgtcg
     9301 tcgacgagct gcacgaacac gtggcgcgcg gagcggttga cgaccagacg cgggcggacg
     9361 tcggtgccga cgatcttctt gcgaagacgc aggtgacgac gcgcgcgggc ggcggacttg
     9421 gacttgacag ccatggttac ttaccagcct ttccggcctt gcgacgcacg acctcgccgg
     9481 cgtaacgcac gcccttgccc ttgtacggct cgggcttgcg gatcttgcgg atgttcgcag
     9541 ctgcctcgcc gacagcctgc ttgctgatgc cgctgacggt cagcttgttc gtgccctcga
     9601 ccgtcagggt gataccggcg ggcgggtcga tcaggaccgg gtgcgagaag ccgagggcga
     9661 actcgaccga gttgcccttc tgctgcacgc ggtaaccggt gccgacgacc tcgaggccct
     9721 tggtgtagcc ctgggtcacg ccgatgatgt cgttgctgat gagcgtgcgg gtcaggccgt
     9781 gaagcgatcg ggatgcgcgc tcgtcgtcgg ggcgggtgac cagaacctgg ttctcctgca
     9841 cctcgacctg gatgggagag gcgaccgtga gggtgagctc acccttgggg cccttcaccg
     9901 cgacctcacg gccgtcgacc gaaacggtca cgcccgcggg gatctcgatg ggaagtcgtc
     9961 cgatacgaga catgtcagat caccacacgt aggcgagaac ttctccgccc acgcccttct
    10021 gctctgcctg acggtccgtg aggagaccgg aggaggtgga caggatggca acacccaggc
    10081 cgccgaggac cttgggcagc tcggtcgact tcgcgtagac gcggagaccg ggcttcgaca
    10141 cgcgcttgat gccggcgatg gagcgctcac ggttgggacc gtacttgagc gacagggtga
    10201 ggctgctgcc gacgcgggcg tcggtcacct cgaagccggc gatgtagccc tccttctgga
    10261 ggatctcggc gatgttcgtc ttgagcttgc tcgacggcat ggtcacggaa tcgtggtgcg
    10321 ccgagttcgc gttgcgcaga cgggtcagca tgtctgcgac cgggtctgtc attgtcatgg
    10381 tgatttcctt cgttcatgag gttccggctg ccgttacacg acagacggcc tgctatgagc
    10441 acggtgtccg agcggacaca caatcttcaa ttgtacggga cgtggggagg gatgcctgac
    10501 gcatccctcc ccacgtctcc gcttacgccg cggcgtcggt cgacttgaac gggaagccga
    10561 gcgccttcag cagcgcgcga ccctcgtcgt ccgtcttggc cgtggtcacc acggtgatgt
    10621 cgaaaccgcg gacgcggtcg atcttgtcct gatcgatctc gtggaacacg ctctgctcct
    10681 ggagaccgaa ggtgtagttg ccgttcccgt cgaactggtc gcccgacaga ccgcggaagt
    10741 cgcggatgcg gggcagcgac aggttcacca gacggtccag gaactcccac gcgcggtcac
    10801 cgcggagggt gacgtgcgcg ccgatggcct gtccctcgcg cagcttgaac tgcgcgatcg
    10861 acttgcgagc cttggtgacg atgggcttct gaccggtgat ccgggtgagg tcctcgaccg
    10921 caccatcgat caccttgctg tcgcgagccg cctcgccgac accggtgttg acaacgacct
    10981 tgaccaggcc ggggatctgc atgacgttgg cgtagccgaa ctgctcctgc agagccttct
    11041 tgatctcggt gttgtacttg gccttcaggc ggggctgcac cttgacagcc tccgcagcct
    11101 cagtcgctgc catcagaggt ccttacctga cttcttcgcg tagcgcacgc ggaccgtgcg
    11161 cttcacgccg tccttgacct gctcctcgac ccggtggccg acgcgggtcg gctgcttggt
    11221 cgaggggtcc acgacggcga cgttggagat gtggatcggg gcctcgacgg tctcgatccc
    11281 acccgtcttg gtgccgcgct gggtctggcc gacacgggtg tgcttggtga cgtagttcac
    11341 gccctccacc acgacgcggt tctgctcgac gaggacctcg aggaccttgc cctgcttgcc
    11401 gcggtcgccg cccttgtcct gcttgcgacc ggtgatgacc tgaaccaggt cgcccttctt
    11461 gatgttcgcc atgatcagat gacctccggg gcgagcgaga cgatcttcat gaacttcttg
    11521 tcgcgaagct cacgaccgac cggtccgaag atacgggtgc cgcggggctc cccgtcgttc
    11581 ttcaggatca cggcggcgtt ctcgtcgaac ttgatgtac
//
LOCUS       DATMSX010000098         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_105694,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000098 DATMSX010000000
VERSION     DATMSX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105694"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN041_03155"
     CDS             complement(<1..300)
                     /locus_tag="VN041_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HWU28075.1"
                     /translation="MGKNYIDIEDDQGETLRYRKHVNGRGLVAHGAKVNPKAVVEAGA
                     YVEPGAKIGAGARVARGAWVDSDAVIGEDAYIDAHAHIGQGAVIGDGAHVGVRTEI"
     gene            complement(403..1176)
                     /locus_tag="VN041_03160"
     CDS             complement(403..1176)
                     /locus_tag="VN041_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWU28076.1"
                     /translation="MPEGDTVFQAARRLDDALRGQQVTRFDLRVPRAALVDLTGSTVH
                     EVVPRGKHILMRIGEHTLHSHLRMDGAWRIYRTGEKWRHPAFKVRAIVGTAAREAVGV
                     DIAEVKVVATRDEGELVGHLGPDPLSDSWDAEDAIRRLSTDDRSIHVALLDQRNVAGF
                     GNEYAAELLFLRGILPTTPTPDVDVPALIDLGVRTIRANRDRIDRTFTGDARRGFTDW
                     VYGRANRPCRRCGTLIRTGALGADPTRERITFWCPNCQR"
     gene            complement(1169..1924)
                     /locus_tag="VN041_03165"
     CDS             complement(1169..1924)
                     /locus_tag="VN041_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HWU28077.1"
                     /translation="MISEFLTGIRYLGRGFGYWKRMPGLMTLGLVPGLIALVVLAAAL
                     IPLWLSLGTITGWITPFADSWDSGWAIALRFAIGAVITIGSLVLASVVFTALALLIGD
                     PFYQRIWRAVENDVGGEVPEGDGGLRVAFGESIRLVLLGILVALLALILGLIPVVGAA
                     VAAVVSVLLTGRLLARELTGRALNARDLPSFRRSQLFATRRSRLLGFGVATQLCFMVP
                     LGAVFIMPTAVAGATLLARELLAQAPDAGAPRA"
     gene            complement(1984..>2327)
                     /locus_tag="VN041_03170"
     CDS             complement(1984..>2327)
                     /locus_tag="VN041_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWU28078.1"
                     /translation="RDAKLTLAAAVVERYHGADAANRERAAFLDVFAHGAVPEELEPV
                     SIEHPASTALALLEQIRPELSRSALRRLIAQDAVRIDGVPVTDPEQQVLVSSGSVVRT
                     GRRSWARVALR"
BASE COUNT          364 a          818 c          822 g          323 t
ORIGIN      
        1 gatctcggtg cggacgccta cgtgcgcgcc gtcgccgatc acggccccct ggccgatgtg
       61 cgcgtgcgcg tcgatgtagg cgtcttcgcc gatcacggca tccgagtcga cccaggcgcc
      121 gcgggcgacg cgcgccccgg ctccgatctt cgccccgggt tcgacatagg ccccggcttc
      181 gacgaccgcc ttggggttca ccttcgcgcc gtgcgcgacg aggccacgac cgttgacgtg
      241 cttgcgataa cgcagcgtct cgccctggtc gtcctcgatg tcgatgtagt tcttgcccac
      301 gattcctccc gtgtccggag acttccggat atagctacaa ccacgggtcg tgagatttca
      361 ttccccaacc gcgatgccct tccgcgggct ccgggactga ggtcatcgct ggcagttcgg
      421 acaccagaac gtgatgcgct cacgcgtcgg atccgcaccg agcgcgcccg tgcggatgag
      481 ggttccgcaa cgccggcacg ggcggttggc gcgaccgtag acccagtcgg tgaagccgcg
      541 gcgcgcgtcg ccggtgaagg tgcgatcgat gcggtcgcgg ttggcgcgga tggtgcgcac
      601 acccaggtcg atgagtgccg ggacgtcgac atccggggtg ggcgtggtgg gcaggatgcc
      661 gcgcaggaac agcagttcgg cggcgtactc gttgccgaag ccggcgacgt tgcgctggtc
      721 gagcagggcg acgtggatgc tgcgatcgtc cgtgctcagc cggcggatcg cgtcctcggc
      781 atcccagctg tccgacagcg gatcaggccc cagatgcccg accagctcgc cctcgtcgcg
      841 agtggcgacg accttcacct cggcgatgtc gacgccgacc gcctcccggg ccgccgtgcc
      901 gacgatcgcg cggaccttga aggccggatg ccgccacttc tcgccggtgc ggtagatgcg
      961 ccacgcgccg tccatgcgca gatgggaatg aagcgtgtgc tcaccgatcc gcatcaggat
     1021 gtgcttgccg cgcggcacga cctcgtgcac ggtgctgcca gtcaggtcga cgagggcagc
     1081 gcgggggacg cgcaggtcga agcgcgtcac ctgctggccg cgcagggcgt cgtcgaggcg
     1141 gcgcgctgcc tggaacaccg tgtcgccctc aggcacgggg agcccccgcg tccggtgcct
     1201 gtgcgagcag ttcgcgcgcc agcagcgtgg caccggcgac agccgtcggc atgatgaaca
     1261 ccgcccccaa cggaaccatg aagcagagct gcgtcgccac cccgaagccg agcagccggg
     1321 agcggcgggt ggcgaacagc tgcgagcgcc ggaagctcgg cagatctcgg gcgttcaatg
     1381 cgcgcccggt cagctcgcgg gccagcagcc ggccggtcag cagcacgctg accacggcgg
     1441 cgacggcagc gcccaccacg gggatgagcc cgaggatcag cgccagcagg gcgaccagga
     1501 tgcccagcag caccaggcgg atgctctcgc cgaaggccac gcgcagcccg ccgtcccctt
     1561 ccgggacctc tccgcccacg tcgttctcga cggcacgcca gatgcgctgg tagaagggat
     1621 caccgatcag cagcgcgagc gcggtgaaga ccacgctggc cagcacgagg gagccgatgg
     1681 tgatcaccgc gccgatcgcg aagcgcaggg cgatggccca gccggagtcc caggaatcag
     1741 cgaacggagt gatccagccc gtgatcgtgc ccagtgagag ccacagcggg atgagtgcgg
     1801 ccgcgagcac caccagggcg atcagcccgg gaaccaggcc gagggtcatc agacccggca
     1861 tccgcttcca gtagccgaag ccgcgcccca ggtaccggat gccggtgagg aactcgctga
     1921 tcatgctcgc cacactatga cactccgccg acccccgatc agtcgccggt tgctcggcgg
     1981 gggctacctc agcgcgaccc gggcccagga gcgcctgccg gtgcggacga cggacccgct
     2041 cgagacgagc acctgctgct cagggtcggt gacggggacg ccgtcgatcc gtacggcgtc
     2101 ctgggcgatc agtcgccgca gggccgacct gctcagttcc ggccggatct gctcgagcag
     2161 ggcgagcgcg gtcgaggccg gatgctcgat gctgaccggc tcgagctcct cgggcaccgc
     2221 tccgtgggcg aacacgtcga ggaacgccgc acgctcccgg ttggcggcat ccgcgccgtg
     2281 gtaccgctcg acgaccgccg ccgcgagggt cagcttcgcg tcgcgcg
//
LOCUS       DATMSX010000099         7939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_143296,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000099 DATMSX010000000
VERSION     DATMSX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143296"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VN041_03175"
     CDS             <1..610
                     /locus_tag="VN041_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HWU28079.1"
                     /translation="APAAAPAPAAAPAAHTVVRGDTLFSIAKKYGTTVSALLQANGLG
                     TGSIIYPGQALRLAPPAPAQRSANLDAAQRANAVRIIQIGRELGVPDRGIAIALATAM
                     VESTMRNLDHGDRDSLGLFQQRPSQGWGTPEQILNADRSIRVFYGGAGDPNGIASKGL
                     LDISGWQGKSFTDAAQSVQVSAYPDRYGLWEQQAHKWVATLR"
     gene            693..2639
                     /gene="pknB"
                     /locus_tag="VN041_03180"
     CDS             693..2639
                     /gene="pknB"
                     /locus_tag="VN041_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWU28080.1"
                     /translation="MNTAQADPLIGRLVDGRYRVRARIARGGMATVYVATDMRLERRI
                     ALKVMHAHLSDDSAFQSRFIQEARSAARLADPHVVNVFDQGQDGDLAYLVMEYLPGIT
                     LRELMREQKRLTVAQTITIMDAILAGLAAAHRAGIVHRDVKPENVLLAEDGRIKIGDF
                     GLARATTANTATGAQLLGTIAYLAPELVTRGTADARSDIYALGIMLYEMLVGEQPYKG
                     EQPMQIAFQHATEQVPRPSVRNPGVPEQLDELVLWATDKSPDERPLNAQEMLDRLREI
                     ERELGVTPVPTATVAPSSGHDSGAVTKILPMGTAVISDPTAPVAAESVDNGARLRQRT
                     ARRRARGALLLSLVLLLAVAAGGVGWWFGSGPGSLIAVPTVAGMPLADAENAIAAENL
                     KSTTTTKSDLNVPAGDVIDSDPGAGERVEKESTVTLIVSSGPAKHTIGALAGLTESAA
                     RQKLDANNLKVDKNVEEYFDKLGPNLVLNVRITPRAGGEPYACGDGCDVFEGDKAQIQ
                     VSLGPVPSVVGKPLDEAKQILESARLKVNPETPEDWSDTVDKGSVIAQSTERQGGGSW
                     RPDDTVTLTVSKGPQPITVPNVVGMTLTKAKAKLESVGLKASYQGWGDLPGFGDLATV
                     IEQKPGDGTLPKGGTVKLTLKLQG"
     gene            complement(2706..4043)
                     /locus_tag="VN041_03185"
     CDS             complement(2706..4043)
                     /locus_tag="VN041_03185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWU28081.1"
                     /translation="MLPSHPELDAWRSLPIKQQPQWPDAERVAEVSSQIATLPPLVFA
                     GEVDNLRTRLARAASGNAFLLQGGDCAETFAGATADQIRNRIKTVLQMAVVLTYGASM
                     PVVKMGRMAGQFAKPRSSDTETRGDVTLPAYRGDIVNGYDFTEKSRQADPGRLLQGYN
                     TAASTLNLIRAFTQGGFADLREVHSWNKGFAQNPANQRYERMAAEIDRAIKFMEAAGA
                     DFDELKRVEFFTGHEGLLMDYERPMTRIDSRTGTPYNTSAHFLWIGERTRDLDEAHVD
                     YFSRIRNPIGVKLGPTTSPDTALALIDKLDPNREPGRLTFITRMGAGKIRDALPPLLQ
                     AIKDSGATPLWVTDPMHGNGITTSTGYKTRRFDDVVDEVRGFFEAHRAVGTFPGGIHV
                     ELTGDDVTECLGGSEHIDEASLATRYESLCDPRLNHMQSLELAFLVAEELEKR"
     gene            complement(4100..4795)
                     /locus_tag="VN041_03190"
     CDS             complement(4100..4795)
                     /locus_tag="VN041_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU28082.1"
                     /translation="MFYWLMKYVAIGPLVKAIFRPWIVGRDNVPASGAAILASNHLSF
                     ADSIFLPLLLDRPMSFLAKSDYFTGRGLKGWATKVFMKGTGQIPIDRSGGKASEASLN
                     TGLQILGRGDLLGIYPEGTRSPDGRLYRGRTGLARMALEAKVPVIPVVMVDTDVIMPI
                     GQSIPNVGRVGVLIGEPLDFSRYAGMENDRYILRAVTDEIMIALQRLGAQEYDDVYAS
                     TVKDRLPQKARRR"
     gene            4913..6739
                     /locus_tag="VN041_03195"
     CDS             4913..6739
                     /locus_tag="VN041_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWU28083.1"
                     /translation="MTQFEAPAIVPADPEANASDLLAIRARETPDLAIFSVPDGAGWR
                     DISAKDFETAVIALAKGFVAAGIQPGEKVGFIAQTTYEWTLVDFALFYAGAVMVPVYE
                     TSSPAQIQWIMEDSGAIALIVESPEHFARVDEVRGDLPLVREVWQLHLGAIDALTAKG
                     AEIEDAEIERRRNLAVGSDIATLIYTSGSTGRPKGCVLTHSNFVELSRNAAESLKHVV
                     YTPGASTLLFITLAHVFARFMSMLYIHAGVRVGHQPDTKQLLASLGSFKPSLLLAVPR
                     VFEKVYNSAEQKAEAGGKGKIFRAAAATAIEHSALQERGEKVPLGLKLKFALFDKLVY
                     SKLRQAMGGNVVYAVSGSAPLGDRLGHFFRSLGVVILEGYGLTETTAPATVNREDGFK
                     IGTVGPVLPGVGIRLADDGEIEVRGICVFKEYWNNPEATAAAFHDGWFRTGDLGSFDS
                     DGFLTITGRKKEIIVTAGGKNVAPAVLEDPIRANPIVGQVVVVGDQKPFIAALVTLDT
                     EMLPTWLANNGLPAEMPLADAAKNPAVHAEVQRAIDRANKSVSRAESIRKFTILPVEW
                     TEASGHLTPKMSIKRNVILADFADQIAEIYEAPVHTTTTPLG"
     gene            complement(6832..7323)
                     /gene="def"
                     /locus_tag="VN041_03200"
     CDS             complement(6832..7323)
                     /gene="def"
                     /locus_tag="VN041_03200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWU28084.1"
                     /translation="MAVREIRLYGDPVLRQVCAPIEQIDDGVRALIADLVDTVELPGR
                     AGVAANQIGVALRAFSYNIDGDIGYVINPVLVEVRGEAVEIGEGCLSVPGLWHDTPRH
                     PWARVEGIDLDGNPVVLEGEGLLAQALQHETDHLDGMVYLGRLQGETRKTAMRQVRES
                     DWF"
     gene            complement(7323..>7939)
                     /locus_tag="VN041_03205"
     CDS             complement(7323..>7939)
                     /locus_tag="VN041_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HWU28085.1"
                     /translation="LAERVVGGHTKSVRDLVRAQHDIRGFHDISALVARDHTRLDVLA
                     SDADPRVSEAFGDADYRDVAAVAAQYYSLVLTDSGTGIVHSVMGATLDLADQIVVVSG
                     LSVDEARLASETLTWLETNGHAQLAREAIVVLNQSTPGTPLVRLSELEAHFSTRAKHV
                     LRIPYDPQIAGGGPIDFTSLQPETRRAAREIAATLVEGLRVKAA"
BASE COUNT         1261 a         2773 c         2702 g         1203 t
ORIGIN      
        1 ggcacccgct gcggctccgg cgcccgcggc ggcacccgcc gctcacacgg tggtgcgcgg
       61 tgacacgctg ttcagcatcg cgaagaagta cggcaccacg gtctccgcgc tcctgcaggc
      121 caacggtctc ggcaccggca gcatcatcta ccctggccag gccctccgcc tcgctccccc
      181 ggcaccggcg cagcgctccg cgaatctgga cgccgcccag cgcgcgaacg cggtccgcat
      241 catccagatc ggccgcgagt tgggggtgcc cgaccgcggc atcgccatcg cgctggcgac
      301 cgcgatggtc gagtccacca tgcggaacct ggaccacggc gaccgcgact ccctcggcct
      361 cttccagcag cgccccagtc agggctgggg aacccccgag cagatcctga acgccgatcg
      421 cagcatccgc gtcttctacg gcggtgcggg cgacccgaac ggcatcgcca gcaagggcct
      481 gctcgacatc tccggctggc agggcaagtc gttcaccgac gcggcgcagt ccgtgcaggt
      541 ctcggcctac ccggaccgct acgggctctg ggagcagcag gcccacaagt gggtcgcgac
      601 cctgcggtga cgcgccagtc gtcaccggat cgaaacctgt cacaggtgcg ccgttgcccg
      661 ggctcacagc ctgttcttca tagactcgtg ctgtgaacac tgcgcaggcc gaccccctca
      721 tcgggcggct tgtcgacggc cggtaccgcg tgcgggcacg catcgcacgc ggcggcatgg
      781 cgacggtcta cgtcgccacc gacatgcgcc tcgaacgacg catcgccctg aaggtgatgc
      841 acgcccacct cagcgacgac tccgccttcc agagcaggtt catccaggag gcccgatcgg
      901 ccgcacggct ggccgatccg cacgtcgtga acgtgttcga ccagggtcag gacggcgacc
      961 tcgcctacct ggtgatggag tatctgcccg gcatcaccct gcgagagctg atgcgcgagc
     1021 agaagcggct gacggtggcc cagacgatca cgatcatgga cgcgatcctc gccggtctcg
     1081 ccgcggcgca ccgcgccggg atcgtgcacc gcgacgtcaa gcccgagaac gtgctgctcg
     1141 cggaggacgg gcgcatcaag atcggggact tcggtctcgc ccgcgccacc accgcgaaca
     1201 ccgccaccgg ggcgcagctg ctgggcacga tcgcgtacct ggcgcccgag ctcgtcaccc
     1261 gcgggaccgc ggatgcccgc agcgacatct acgcgctggg catcatgctg tacgagatgc
     1321 tcgtcggcga gcagccgtac aagggcgagc agcccatgca gatcgcgttc cagcacgcca
     1381 ccgaacaggt gccgcgcccc agtgtccgca atcccggagt acccgagcag ctcgatgagc
     1441 tcgtgctctg ggcgacagac aagtctcccg acgagcgacc actgaacgcg caggagatgc
     1501 tcgaccgcct ccgcgagatc gagcgcgagc tcggtgtcac gcccgtgccg acggcgacgg
     1561 tcgcaccgtc gtccggtcac gattccggag cggtcaccaa gatcctcccg atgggcacgg
     1621 cggtcatctc cgaccccacg gctcccgtcg ccgcggagtc cgtcgacaac ggcgcccgcc
     1681 tgcggcagcg cacggcacgt cggcgcgccc ggggtgcgct gctgctctca ctggtgctgc
     1741 tgctcgccgt cgccgcgggc ggcgtcggct ggtggttcgg ctccggaccc ggatcgctga
     1801 tcgccgtccc gacggtggca gggatgccgc tggcggacgc cgagaacgcc atcgccgccg
     1861 agaacctgaa gtcgacgacg accacgaagt ccgacctcaa cgtgcctgcc ggcgacgtga
     1921 tcgattccga tcccggggcg ggcgaacgcg tcgagaagga gagcacggtc acgctgatcg
     1981 tgtcgtcagg ccccgccaag cacaccatcg gcgcgctggc cggcctgacc gagtccgccg
     2041 cgcgtcagaa gctcgacgcc aacaacctca aggtcgacaa gaacgtcgag gagtacttcg
     2101 acaagctcgg cccgaacctc gtgctgaacg tgcggatcac cccacgcgcg ggcggcgagc
     2161 cctacgcctg cggcgacggc tgcgacgtct tcgagggcga caaggcgcag atccaggtct
     2221 ccctgggccc ggtcccgagc gtcgtcggca agccgctcga cgaggcgaag cagatcctgg
     2281 aatccgcccg gctgaaggtg aacccggaga cgccggagga ctggagcgac accgtcgaca
     2341 agggctccgt gatcgcgcag agcacggagc gccagggcgg cggcagttgg cgccccgacg
     2401 acaccgtgac cctgaccgtc tccaagggcc cgcagcccat caccgtgccg aacgtcgtcg
     2461 gtatgacgct caccaaggcc aaggccaagc tggagagcgt cggcctgaag gcgtcctacc
     2521 agggctgggg cgatcttccc ggtttcggcg acctcgcgac ggtcatcgag cagaagcccg
     2581 gggacgggac gctgcccaag ggcggcacgg tcaagctcac cctcaagctg cagggctgat
     2641 cgttccgcgg atgctgaccg ccgcgcgcgg cgacgatcgg catccgcgga gccatccgcc
     2701 ggagctcagc gcttctcgag ctcctcggcc acgaggaacg cgagctccag gctctgcatg
     2761 tggttcagtc gcgggtcgca caggctctcg tagcgcgtcg cgagggatgc ctcgtcgatg
     2821 tgctccgagc cgcccaggca ctcggtgacg tcgtcgccgg tgagctcgac gtggatgccg
     2881 ccggggaacg tgcccacggc gcggtgcgct tcgaagaagc cgcgcacctc gtcgaccacg
     2941 tcgtcgaagc gacgggtctt gtagccggtc gaggtggtga tgccattgcc gtgcatgggg
     3001 tccgtcaccc acagcggggt ggcgccggag tccttgatcg cctgcagcag cggcggcagg
     3061 gcatcccgga tcttcccggc ccccatccgg gtgatgaagg tgagacggcc cggctcgcgg
     3121 ttcgggtcga gcttgtcgat cagcgcgagg gcggtgtcgg gagacgtggt cgggcccagc
     3181 ttgacgccga tggggttgcg gatccgcgag aagtagtcga cgtgcgcctc atcgaggtcg
     3241 cgggtgcgct ctccgatcca caggaagtgg gcagacgtgt tgtacggcgt gcccgtgcgg
     3301 gagtcgatgc gcgtcatggg gcgctcgtag tccatcagca ggccctcgtg ccccgtgaag
     3361 aactcgacgc gcttgagctc gtcgaagtcg gcgccggcgg cctccatgaa cttgatggcg
     3421 cggtcgatct cggccgccat gcgctcgtaa cgctggttgg ccgggttctg cgcgaatccc
     3481 ttgttccagg agtgcacctc gcgcagatcg gcgaaaccgc cctgcgtgaa cgcccggatg
     3541 aggttcagtg tggatgccgc cgtgttgtaa ccctgcagca gacggcccgg atcggcttga
     3601 cgggacttct cggtgaagtc gtagccgttg acgatgtcac ctcggtaggc cggcagtgtg
     3661 acgtcgccgc gggtctcggt gtcgctggag cgcggcttgg cgaactggcc ggccatccgc
     3721 cccatcttca ccaccggcat cgaggcgccg taggtgagca cgacggccat ctgcagcacg
     3781 gtcttgatgc ggttgcggat ctgatcggcc gtcgcaccgg cgaaggtctc agcgcagtcg
     3841 ccgccctgca gcaggaacgc gttgcccgag gccgctctgg ccagtctggt gcggaggttg
     3901 tcgacctcgc cggcgaagac cagcggcggc agggtcgcga tctgggagga gacctccgcc
     3961 acccgctcgg cgtcgggcca ctgcggctgc tgcttgatcg ggagggaccg ccacgcgtcg
     4021 agttcagggt gcgagggaag catccaccca gcttagtgtc cgcccgaggg cgctccccgc
     4081 gctgtgacgg ggccgcgatt cagcggcgtc gggccttctg cggcagccgg tccttcactg
     4141 tggacgcata gacgtcgtcg tactcctggg cacccaggcg ctgcagcgcg atcatgatct
     4201 cgtcggtgac ggcgcgcagg atgtagcggt cgttctccat gcccgcatag cgggagaagt
     4261 ccaggggctc cccgatcagc acgcccacgc gtccgacatt cgggatgctc tgcccgatcg
     4321 gcatgatgac gtcggtgtcg accatgacga cggggatcac cgggaccttc gcctccagcg
     4381 ccatgcgagc caagcccgtg cgaccgcggt acagcctgcc gtcggggctg cgggtgccct
     4441 cgggatagat gccgagcagg tcgccccggc ccaggatctg cagaccggtg ttgagggacg
     4501 cctcggacgc cttgccgccg gagcggtcga tggggatctg gcccgtgccc ttcatgaaca
     4561 ccttggtggc ccagcccttg aggccacggc cggtgaagta gtcgctcttg gcgaggaagg
     4621 acatcggccg gtccagcagc agcggcagga agatggagtc ggcgaacgac agatggttgc
     4681 tcgcgaggat ggcagcaccc gacgcgggga cgttgtcgcg ccccacgatc cagggtcgga
     4741 agatcgcctt gaccagcggc ccgatcgcga cgtacttcat cagccagtag aacatcgtgc
     4801 agaagtctag cctcgccggc accctcgcga ccgagtttca ggttttctgc gactggatgc
     4861 catgacctag actcggggtg tcacccgtca atccggtacc gaaggagctg ccgtgaccca
     4921 gttcgaagcc cccgccatcg ttcccgccga tcccgaggcg aacgcgtccg acctgctcgc
     4981 gatccgcgcc agggagacgc cggatctggc catcttctcc gttcccgacg gcgccggctg
     5041 gcgcgacatc tcggccaagg acttcgagac ggcggtgatc gccctcgcca aggggttcgt
     5101 ggccgcaggc atccagcccg gcgagaaggt cggcttcatc gcgcagacca cctacgagtg
     5161 gaccctcgtc gacttcgcgc tgttctacgc gggcgccgtc atggtcccgg tctacgagac
     5221 cagctcgccc gcgcagatcc agtggatcat ggaggactcc ggggcgatcg cgctcatcgt
     5281 cgagtctccc gagcacttcg cccgcgtgga cgaggtgcgc ggcgacctgc ccctggtgcg
     5341 cgaggtgtgg cagctgcacc tgggcgcgat cgacgcgctc accgcgaagg gcgccgagat
     5401 cgaggatgcc gagatcgagc gccgccgcaa cctggcggtc ggctccgaca tcgcgacgct
     5461 gatctacacc tccggttcga ccgggcgtcc gaagggctgc gtgctcacgc acagcaactt
     5521 cgtcgagctc tcgcggaacg ccgccgaatc cctcaagcac gtcgtgtaca cccccggcgc
     5581 ctccaccctg ctgttcatca cgctcgcgca cgtgttcgcg cggttcatgt cgatgctcta
     5641 catccacgcg ggcgtgcgcg tgggtcatca gcccgacacg aagcagctgc tcgcctcgct
     5701 cggctccttc aagccgtcgc tgctgctcgc ggtcccccgc gtgttcgaga aggtctacaa
     5761 ctcggccgag cagaaggccg aggccggcgg caagggcaag atcttccgcg cagccgccgc
     5821 caccgcgatc gagcactccg ccctgcagga gcgcggcgag aaggtgccgc tcggactgaa
     5881 gctgaagttc gccctgttcg acaagctggt gtactccaag ctgcgccagg ccatgggcgg
     5941 caacgtcgtc tacgccgtct ccggatccgc tccgctgggc gaccgtctcg ggcacttctt
     6001 ccgcagcctc ggcgttgtca tcctcgaggg ctacggcctc accgagacga ccgcccctgc
     6061 gaccgtgaac cgcgaggacg gcttcaagat cggcacggtc ggtccggttc ttcccggcgt
     6121 cggcatccgt ctcgccgacg acggcgagat cgaggtccgc ggcatctgcg tgttcaagga
     6181 gtactggaac aaccccgagg cgacagccgc ggcgttccac gacggctggt tccgcaccgg
     6241 tgacctgggc tccttcgact cggacggctt cctgacgatc acgggccgca agaaggagat
     6301 catcgtgacc gccggaggca agaacgtcgc cccggccgtg ctcgaggacc cgatccgcgc
     6361 gaacccgatc gtcgggcagg tcgtcgtggt cggagaccag aagccgttca tcgcggcgct
     6421 ggtgacgctg gacaccgaga tgctgccgac ctggctggcc aacaacgggc tccccgcgga
     6481 gatgcccctg gcggatgccg cgaagaaccc cgctgtgcac gccgaggtgc agcgcgcgat
     6541 cgaccgcgcg aacaagtcgg tctcgcgcgc cgagtcgatt cgcaagttca cgatcctgcc
     6601 cgtggagtgg accgaggcga gcggtcacct gactccgaag atgtccatca agcgcaacgt
     6661 gatcctcgcg gacttcgccg accagatcgc cgagatctac gaggcgccgg tgcacaccac
     6721 cacgacgccc ctgggctgac ccgggcaaga catgaagagg aggccctccg ccgagcggag
     6781 ggcctcctct tcgttcgtgt cctggggttc cgggttctgg acctcggtgc gctagaacca
     6841 gtcgctctcg cgcacctgcc gcatcgcggt cttcctggtc tccccctgga gcctgcccag
     6901 gtacaccatg ccgtccaggt ggtcggtctc gtgctgcagg gcctgggcga gcagcccctc
     6961 cccttccagt acgaccgggt tgccgtcgag gtcgatgccc tcgacccgag cccacgggtg
     7021 acgcggcgtg tcgtgccaca ggccgggcac cgacaggcat ccctcgccga tctcgacagc
     7081 ctcgccgcgc acctcgacca gcacgggatt gatgacgtag ccgatgtcac cgtcgatgtt
     7141 gtagctgaac gcacgcagcg cgacaccgat ctggttcgcg gcgacgcctg cccggccggg
     7201 cagctcgacg gtgtcgacga ggtcggcgat cagagcgcgc acgccgtcgt cgatctgctc
     7261 gatcggggcg cacacctggc gcagcacggg gtcgccgtac aggcggatct cacggacggc
     7321 catcaggcgg ccttcacccg caggccctcg acgagcgtgg ccgcgatctc gcgagccgcg
     7381 cgccgggtct caggctggag gctggtgaag tcgatggggc caccgccggc gatctgaggg
     7441 tcgtacggga tgcgcagaac gtgcttcgcg cgcgtcgaga agtgcgcctc cagctcgctc
     7501 aggcgcacga gcggggtgcc gggcgtcgac tggttcagca ccacgatggc ctcccgcgcg
     7561 agctgcgcgt ggccgttggt ctccagccag gtcagggtct cggacgccag tcgggcctca
     7621 tcgacgctga gcccggagac gacgacgatc tgatcggcca ggtcgagcgt ggcacccatc
     7681 acggagtgca cgatgccggt gcccgagtcg gtcaggacca gcgagtagta ctgcgccgcc
     7741 accgccgcga cgtcacggta gtccgcatcg ccgaaggcct ccgagacgcg cggatcggcg
     7801 tcggaggcga gcacgtccag ccgggtgtgg tcgcgtgcga ccagcgcgga gatgtcgtgg
     7861 aagccgcgga tgtcgtgctg cgcgcgcacg aggtcgcgca ccgacttggt gtggccgccg
     7921 accacgcgct cggcgagcg
//
LOCUS       DATMSX010000100         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_86792,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000100 DATMSX010000000
VERSION     DATMSX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86792"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VN041_03210"
     CDS             complement(<1..432)
                     /locus_tag="VN041_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HWU28086.1"
                     /translation="MEPAAAPTLRTLLGRRDLGLHLVSDESELVDGALEQPVRWVHNS
                     DLVDPTPFLAEDLVLLTTGMQLAEEDAEGAAAYVARLVHRGVLALGFGSGVHRVGVPD
                     ELIAACAHAGLALFEVPYDIPFLAIARAHAEAIAAAAYARRT"
     gene            526..1893
                     /gene="gabT"
                     /locus_tag="VN041_03215"
     CDS             526..1893
                     /gene="gabT"
                     /locus_tag="VN041_03215"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWU28087.1"
                     /translation="MTLIDETSTIPAGGPTLEQVRRVVTELPGPRSAAILARKADAVA
                     GGVSTSMPVAVVAAGGGVVLDADGNSLIDLGSGIAVTSVGNAHPKVAAAVAAQAAQFT
                     HTCFMISPYESYIEVAEALNRLTPGDFAKKTALFNSGAEAVENAIKIARKHTGRQAVV
                     AFDHGYHGRTNLTMALTAKSMPYKSGFGPFAPEVYRVPASYPYRDGLSGAEAAKRAIT
                     LIEKQVGADNLAAVIIEPIQGEGGFIVPADGFLEAIVEWSRANGVVFIADEVQTGFAR
                     TGAMFASEIFGIEPDLITTAKGIAAGLPLAGVTGRAEIMDAAHAGGLGGTYGGNPVAC
                     AAALAAIDAFENEGLVERAQQIGSILRDRLEAIKATDPRIGDVRGHGAMMAAEFVNPE
                     TGEPDAALTGAVAKACTAEGVIVLTCGTFGNVIRFLPPLSIGDELLNDGLDVVAAALA
                     NAQGN"
     gene            1983..>3340
                     /locus_tag="VN041_03220"
     CDS             1983..>3340
                     /locus_tag="VN041_03220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU28088.1"
                     /translation="MSEHEISRDVVIIGAGAAGLTAANELRRAGLSVAVLEARDRVGG
                     RLWTDVIDGAMLELGGQWISPDQDALKQTVEELGLETFSRYREGDSVYVGPDGVVHRF
                     TGEMFPVSPATEKVIAEVTERLDAMVAEIDPDRPWEHPNAAEWDSITWDAWLRTQTDD
                     DEAIRNLAFATGSAMLTKPTHAFSLLQSLLMAASAGSYSNLVDADFILDKRVVGGLQQ
                     VPLLLAERLGDDVLLGQPVRSLEWSDPSSGSGTGGVVAVTDKTTVRARFAILAHAPVL
                     YNRISFVPPLPRRQHQLHQHLSMGFVIKVHAVYDRPFWREQGLSGTAFSPYELSHEAY
                     DNTNHGDERGTLVGFVSDRNADDLFALSAEERKERILESLSHYYGPEAKNPVVYYESD
                     WGTEEWTRGAYAASFDMGGLHRYGADLRSAVGPIHFACSDMAGAGYQHVDGAIRMGHL
                     VAS"
BASE COUNT          519 a         1251 c         1111 g          459 t
ORIGIN      
        1 ggtgcgccgg gcgtacgcgg cggccgcgat ggcttcggca tgcgcgcggg cgatcgcgag
       61 gaacgggatg tcgtagggga cctcgaacag tgcgagccct gcgtgcgcgc acgcggcgat
      121 gagctcgtcg ggtacgccga cgcggtgcac gccggagccg aaccccagag ccagcacgcc
      181 gcggtggacc aggcgtgcga cgtacgcggc ggcgccctcg gcgtcctcct cggcgagctg
      241 catgccggtg gtcagcagca ccaggtcctc ggcgaggaac ggcgtggggt ccaccagatc
      301 ggagttgtgc acccagcgaa cgggctgctc cagggcgccg tcgaccagct ccgattcgtc
      361 ggacaccagg tgcagcccga ggtcgcgccg gccgagcaga gtgcgcaggg tcggtgcggc
      421 ggcgggttcc atggcatctc caggatgtac gaagcggcga aatgctcagc agtattgtac
      481 gggtaggcga atgcatccgc gtgcggccgg acgtagcctc gcctcatgac actcatcgac
      541 gagacctcca cgatccccgc cggcggaccc accctcgagc aggtccgtcg cgtcgtcacc
      601 gaactccccg ggccccgctc ggccgcgatc ctcgcccgca aggcggatgc cgtcgccggc
      661 ggcgtctcca cgtccatgcc ggtcgccgtc gtggccgcgg gtggcggagt ggtcctcgac
      721 gccgacggca actcgctcat cgacctcggc tccggcatcg ccgtgaccag cgtcggcaac
      781 gcgcacccca aggtcgccgc cgcggtcgca gcccaggcag cgcagttcac ccacacctgc
      841 ttcatgatct cgccctacga gtcctacatc gaggtggccg aggcgctgaa ccgcctgacc
      901 cccggcgact tcgccaagaa gacggcgctg ttcaactccg gcgccgaggc cgtcgagaac
      961 gcgatcaaga tcgcccgcaa gcacaccgga cgccaggccg tcgtcgcctt cgaccacggc
     1021 taccacggcc gcaccaacct cacgatggcg ctgaccgcga agtcgatgcc gtacaagtcc
     1081 ggcttcgggc cgttcgcccc cgaggtctac cgcgtgcccg cttcgtaccc ctaccgcgac
     1141 ggcctgtccg gcgccgaggc cgccaagcgc gccatcacgc tcatcgagaa gcaggtcggc
     1201 gccgacaacc tggccgccgt catcatcgag cccatccagg gcgagggcgg cttcatcgtc
     1261 cccgccgacg ggttcctgga ggccatcgtc gagtggagcc gcgccaacgg cgtcgtcttc
     1321 atcgccgacg aggtgcagac cggcttcgcc cgcaccggcg ccatgttcgc cagcgagatc
     1381 ttcggcatcg agcccgatct catcaccacc gccaagggca tcgccgccgg tctcccgctc
     1441 gccggcgtca ccggccgtgc cgagatcatg gacgccgcgc acgctggtgg cctgggcggc
     1501 acctacggcg gcaaccccgt cgcctgcgcc gcagcactgg cagcgatcga cgccttcgag
     1561 aacgagggcc tcgtcgagcg cgcgcagcag atcggcagca tcctgcgcga ccgcctcgag
     1621 gccatcaagg cgaccgaccc gcgcatcggc gacgtccgcg gccacggcgc gatgatggca
     1681 gccgagttcg tcaaccccga gacgggggag ccggatgccg cgctcaccgg cgccgtcgcc
     1741 aaggcctgca ccgccgaggg cgtcatcgtc ctgacctgcg gcaccttcgg caacgtcatc
     1801 cgcttcctgc cgcctctgtc gatcggcgac gagctgctga acgacggtct ggacgtcgtc
     1861 gcggccgcgc tcgcgaacgc ccagggcaac tgagaccgcc ggccgcgatc ctggtcgggg
     1921 gatcagggat cgcggccggc agcaccttca cacctcaccc tctgcatcaa aggagatcgc
     1981 tcatgagcga gcacgagatc agccgcgacg tcgtcatcat cggagccggc gccgccggcc
     2041 tcaccgcagc caacgagctg cgcagggcgg gcctctccgt cgccgtgctg gaggcgcgcg
     2101 accgcgtcgg cggccggctg tggaccgacg tcatcgacgg cgccatgctc gagctcggcg
     2161 gccagtggat ctcgccggat caggacgccc tgaagcagac ggtcgaggag ctcggcctgg
     2221 agaccttcag ccgctaccgc gagggtgaca gcgtgtatgt cggccccgac ggagtcgtgc
     2281 accgcttcac cggcgagatg ttccccgtgt cgcccgcgac cgagaaggtc atcgccgagg
     2341 tcaccgagcg tctcgacgcg atggtcgccg agatcgaccc cgaccgcccc tgggagcacc
     2401 cgaacgcggc ggagtgggac agcatcacct gggacgcctg gttgcgcacg cagaccgacg
     2461 acgacgaggc gatccgcaac ctcgccttcg ccacgggctc ggccatgctc accaagccga
     2521 cgcacgcgtt ctcgctgctg cagtcgctgc tgatggcggc atccgcgggc tcctactcga
     2581 acctcgtcga cgccgacttc atcctcgaca agcgcgtcgt cggaggcctg cagcaggttc
     2641 cgctgctgct cgccgagcgc ctcggcgacg acgtgctgct ggggcagccg gtccgcagcc
     2701 tggagtggtc cgacccctcg tccggttcgg ggaccggcgg cgtcgtcgcc gtcaccgaca
     2761 agaccaccgt gcgcgcgcgc ttcgcgatcc tcgcgcacgc cccggtgctc tacaaccgga
     2821 tctcgttcgt cccgccgctg ccgcgtcgcc agcatcagct gcaccagcat ctgtcgatgg
     2881 gcttcgtcat caaggtgcac gcggtgtacg accgtccgtt ctggcgcgag cagggcctca
     2941 gcggcaccgc gttcagcccg tacgagctct cgcacgaggc ctacgacaac accaaccacg
     3001 gcgatgagcg cggcacgctg gtcggcttcg tcagcgaccg caacgccgac gacctgttcg
     3061 cgctgagcgc cgaggagcgc aaggagcgca tcctcgagtc gctgtcgcac tactacggcc
     3121 ccgaggcgaa gaacccggtc gtctactacg agagcgactg gggcaccgag gagtggaccc
     3181 ggggcgccta cgccgccagc ttcgacatgg gcgggctgca ccgctacggc gccgacctgc
     3241 gctccgccgt cggcccgatc cacttcgcct gcagcgacat ggccggcgcc ggctaccagc
     3301 acgtcgacgg cgccatccgg atggggcacc tggtcgcctc
//
LOCUS       DATMSX010000101         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124770,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000101 DATMSX010000000
VERSION     DATMSX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124770"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..669
                     /locus_tag="VN041_03225"
     CDS             169..669
                     /locus_tag="VN041_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWU28089.1"
                     /translation="MTDVPLTAPPTDAWQSLPRRASRLAALEGAFGGLFVPGLPLAAA
                     WWFFDLPGGVWVAALGLLVGAACGAWLGHRRLVRTRWRLDAQGLGLRRDLMWQLETRI
                     PLSRVQHLDLRRGPLERRAGLATLIVHTAGTRMSAVTVSGLDEADAERLRDTLSHQLD
                     QDADAL"
     gene            666..>1886
                     /locus_tag="VN041_03230"
     CDS             666..>1886
                     /locus_tag="VN041_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWU28090.1"
                     /translation="MSLPPPLPAALPTGGEDHRLHPWSWLFVLLMQLRHYFLPLVALL
                     VFGQRGDRDPMWAQLIPLTAIAALVLVSVLQYLSYRYRIGSDAITVRSGLLARNRREI
                     PFARIHNVEVRQNPLHRLFGVAELRLESAGGVRPEAEMRVLKLDQALALERLVRQRGQ
                     APQAADVVAAASITEADTEQVLLRLSSWDVVRMGLLSNRGWALAIAAFGVLFQTVPRP
                     VMDDALQRGGREAFGYASHLHPGVAGAFLLLAAALLLGWLALRVLSVVLTLLRYHGFT
                     LSEQDRRLTVSAGLLSRTRSSVARRRIQAWTLREGTLQRWFGLRQLRIDSAAGGPARD
                     EDRALRELAPLASSTRCEQLVQHLLPQLQWPPAQWQSIPQRGWWRLCLGALLLVPSSL
                     SSGERCRRRPCTLSS"
BASE COUNT          234 a          674 c          659 g          319 t
ORIGIN      
        1 atcaccgccg agaacgaaca ctacctgcgc accaaggccg atcgtgccgg ccacatgctg
       61 gatgtggacg cgctgatcca ggctgcccag taacgcagcc gacctgctac cgtctgcgcg
      121 gtgcggccac ggccgccacc gcccgccctg ccacctgacg gagcccgcgt gaccgacgtt
      181 ccccttactg ctccgcccac cgatgcctgg cagtcgctgc cgcgccgcgc ctcgcggctg
      241 gccgcactgg aaggtgcctt cggtggcctg tttgtgccag gcctgccgct ggccgctgca
      301 tggtggttct tcgatctgcc cggtggcgta tgggtcgcag ccctcgggct gctggtgggg
      361 gctgcatgtg gtgcgtggct cggccaccgc cgcctggtcc gcacccgttg gcggctggac
      421 gcacagggcc tgggcctgcg acgagacctg atgtggcagc tggaaacgcg cattcccctg
      481 tcgcgcgtgc agcacctgga cctgcgccgc gggccactgg aacgccgcgc cggtctggcc
      541 acgctgatcg tgcacaccgc cggcacccgc atgagcgcgg tgaccgtcag cggcctggac
      601 gaggcggatg ccgagcgcct gcgcgacacc ctgtcccatc agctcgacca ggacgccgac
      661 gccctgtgag cctgccacca cctctgcctg cagcgctgcc caccggtggc gaggaccacc
      721 gcctgcatcc gtggtcatgg ttgttcgtac tgttgatgca gctgcgccat tacttcctgc
      781 cgctggtcgc gctgctggtg ttcggccagc ggggtgaccg cgacccgatg tgggcgcagc
      841 tgattccgct gacggcgatt gccgcactgg tactggtgtc ggtgctgcag tacctcagct
      901 accgctaccg catcggcagc gatgcgatta ccgtgcgcag cggtctgctg gcgcgcaacc
      961 ggcgcgagat tccgtttgcg cgcatccaca acgtggaggt ccggcagaac ccgctgcacc
     1021 gcctgttcgg tgtggccgag ctgcgcctgg aatcggccgg cggcgtgcgt ccagaagccg
     1081 aaatgcgggt gctgaagctg gaccaggcgc tggcgctgga acgactggtg cgccagcgcg
     1141 ggcaggcgcc gcaggccgcc gacgttgtcg ccgccgcctc gatcaccgag gccgacaccg
     1201 aacaggtact gctgcgcctg tcgagctggg acgtggtgcg catggggctg ctctccaacc
     1261 gtggctgggc gctggcgatc gccgcgttcg gtgtcctgtt ccagaccgtc ccgcggccgg
     1321 tgatggacga tgccctccag cgcggtggcc gcgaggcctt cggctacgcc agccacctgc
     1381 accccggcgt ggccggcgcg ttcctgctgc tggctgcggc tctgttgctg ggctggctgg
     1441 cgctgcgcgt gttgtcggtg gtgctgaccc tgctgcgcta ccacggcttc accctcagcg
     1501 agcaggatcg ccgcctgacc gtttcggctg gcctgctcag ccgcacccgc agcagcgtgg
     1561 cgcgccggcg catccaggcc tggacgctgc gcgaaggtac gttgcagcgc tggttcggtc
     1621 tgcgccagct gcgcatcgac agcgccgccg gtggccctgc gcgcgatgag gaccgcgccc
     1681 tgcgcgaact ggcaccgctg gcgtcaagta cgcgttgcga gcagctggtg cagcacctgc
     1741 tgccacagtt gcagtggcca cccgcgcagt ggcagtccat tccgcagcgc ggctggtggc
     1801 ggctgtgcct gggcgcgctg ctgctggtgc cgagcagctt gtccagcggt gagcgctgca
     1861 ggcgcaggcc ctgcaccttg tccagc
//
LOCUS       DATMSX010000102         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_67846,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000102 DATMSX010000000
VERSION     DATMSX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67846"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VN041_03235"
     CDS             complement(<1..1180)
                     /locus_tag="VN041_03235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWU28091.1"
                     /translation="MRVAIVTESFLPHMNGVTGSVLQILRHLERTGHQAHVIAPSAPD
                     TPLRSGGARVDTVASVPLPGYRHVRVGMASTGRVVDSLRRFQPDVVHLASPFALGWRG
                     VVAAQRLALPTVAAYQTDVAAYTSRYGVALTTSLAESHLARLHRRATLTLAPSSESEA
                     QLSRLGVDRVRRWGRGVDAERFHPDAGDEQWEHRSPGLVTVGYVGRLAPEKQVEDLRA
                     LRGIAGIRLVIVGDGPSRARLESLLPDTLFLGHLSGTELARAMASFDIFVHPGESETF
                     GQTLQEAHASGVPVIATGRGGPLDLVRMGIDGWLYRPGDLDDLRMRVRDLAGDERKRR
                     AFGRAGREAVEGRSWTAVCEQLMERFEEAQRLHALDRTLRVPRTRRPEPAEPQRGRRW
                     Q"
     gene            1303..>1577
                     /locus_tag="VN041_03240"
     CDS             1303..>1577
                     /locus_tag="VN041_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28092.1"
                     /translation="MSDSSAVMPEPLLQLLTGPWGLVVMCALVVGDAFLVVIPGEIAV
                     TALGAIAMSQGSPPLSAVIGLAAAAAWCGDVACYLVGRTAGVERWRW"
BASE COUNT          214 a          569 c          566 g          228 t
ORIGIN      
        1 gctgccagcg cctcccccgt tgcggttcgg cgggctccgg tcgccgcgtc cggggaacgc
       61 gcagcgtgcg atccagcgcg tgcagccgct gcgcctcctc gaatctctcc atgagctgtt
      121 cgcagacagc cgtccagctg cggccctcca cggcctcgcg cccggcccgg ccgaacgcgc
      181 ggcgcttgcg ctcgtctccc gcgagatcgc gcacccgcat ccgcagatcg tccaggtccc
      241 cgggccggta cagccagccg tcgatgccca tgcgcaccag atccagtgga ccgccgcgcc
      301 cggtggcgat caccgggacg ccgctggcgt gcgcctcctg cagggtctgc ccgaaggtct
      361 cgctctcgcc ggggtgaacg aagatgtcga aggatgccat cgcccgggcg agctcggtgc
      421 cggagaggtg gccgaggaac agggtatccg ggagcaggga ctccaggcgg gcacgggacg
      481 gcccgtcgcc gacgatcacc aggcggatgc ccgcaatgcc gcgcagcgca cgcaggtcct
      541 ccacctgctt ctccggcgcg agacggccca cgtagcccac cgtcaccagg ccggggctgc
      601 gatgctccca ctgctcgtct ccggcatcgg ggtggaagcg ctcggcatcc actcccctgc
      661 cccaccggcg caccctgtcc actccgagcc tgctgagctg cgcctcggac tcgctcgacg
      721 gcgccagcgt cagagtggcg cggcggtgca gccgtgcgag atgactctcg gcgagggagg
      781 tggtcagcgc caccccgtag cgcgacgtgt acgccgcgac gtcggtctgg tacgcggcca
      841 ccgtgggcag ggcgagccgt tgggccgcca cgacgccgcg ccagccgagg gcgaacggcg
      901 atgccaggtg gacgacgtcc ggctggaacc ggcgcagcga atcgacgacg cgccccgtcg
      961 atgccattcc cacccgcaca tgccgatagc cgggcagcgg cacggatgcc acggtgtcga
     1021 cgcgcgcgcc accggagcgc agcggcgtgt caggagcgga cggggcgatc acgtgcgcct
     1081 gatgcccggt gcgctcgagg tggcgcagga tctgcagcac ggatccggtc acgccgttca
     1141 tgtgcgggag gaaggactcg gtgacgatcg cgactctcac caggccagga tgcccgggcg
     1201 agacgcacgg aacgggtgga atcggcgtcg ggagccgagt gttcacccga tgcacggatg
     1261 ccggtcatct cctcacctgc cggcctcctt ccgttcacca ggatgagcga cagttccgct
     1321 gtgatgccgg aacctctgct gcagctgctg accgggccct ggggtctcgt ggtgatgtgc
     1381 gctctcgtcg tcggagacgc gttcctggtc gtcatccccg gtgagatcgc cgtcaccgcg
     1441 ctcggcgcga tcgcgatgtc gcaggggtct ccgccgctgt cggccgtgat cgggctggcc
     1501 gcggccgccg cctggtgcgg cgatgtcgcc tgctatctgg tcgggcgcac ggcgggcgtg
     1561 gagcgctggc gctggat
//
LOCUS       DATMSX010000103         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172103,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000103 DATMSX010000000
VERSION     DATMSX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172103"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VN041_03245"
     CDS             complement(<1..751)
                     /locus_tag="VN041_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HWU28093.1"
                     /translation="MSVGLLAVVDDILSAAVKASAKAAGVVIDDAAVTPQYVQGLTPA
                     RELPVVGKIALGSIANKFIVIIPIALLLTAFAPGVLPYLLIVGGTYLCFEGAEKVLEW
                     FGFGHGHGDEGERDENRLVWGAIRTDLILSTEIMLISLASLDKNLGIWQTLGVLAVIA
                     LIMTGVVYGAVALLVKIDDIGLGMAKSPVRRVRHTGARIARSMPAVFRVISVVGTVAM
                     LWVGGHLAIVNLGEVGLHWPADLLHGVDELLA"
     gene            complement(810..2225)
                     /locus_tag="VN041_03250"
     CDS             complement(810..2225)
                     /locus_tag="VN041_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWU28094.1"
                     /translation="MTEVPAITTTTQGLHPGLTRRQISMMGLGGAIGAGLFVGSGEAI
                     SIAGPAVLVSYLVAGTIVILVMSMLAEMVAARPSSGAFSSFAQRAMGRSAGSAVGWLY
                     WIQLVVVIAAEATGAAGIVQGWTPGFPAWAWVLVFVAALTAVNLFGVRNYGRFEFWFA
                     AIKVFAIIAFLIVGACAIFGLIPNVPAVGVGNLVAHGGFAPHGITGIAAALLIVVFAF
                     GGTEVVAIAAAESDDPANNIRRIVREVMVRILIFYMGSIFVIVAVLPWDEKEVKAGPF
                     SAVLATLHLPGVDLLMSAIVVLALLSAMNANIYGASRMVYSLGERGLAPLAAIRTSAK
                     GVPYVAVLASVAFGFVTVGLNWAFPEVVLGLLLNIVGSTVLVIWTATAISQIILRRRA
                     DRDGEEMPMRMWGFPVLSWACLVLLAVIIALAMIDQAARTQLLLTIGLTAVLLAVARL
                     TRGSSRPGVPRALTEQHPPTR"
     gene            complement(2398..>2759)
                     /locus_tag="VN041_03255"
     CDS             complement(2398..>2759)
                     /locus_tag="VN041_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28095.1"
                     /translation="GAGAMITVDPSGALAPFEQIRSQLADGIRSGELENGHRLPSIRQ
                     LAGDLRVAAGTVAKAYALLEEEGLVETSRARGTRVSAGHAHPDAVRDAARAFVAASAG
                     LSLDEALGAVRAAWHAG"
BASE COUNT          471 a          973 c          955 g          360 t
ORIGIN      
        1 gggcgagcag ctcgtccacg ccgtgcagca ggtcggccgg ccagtgcagg ccgacctcgc
       61 cgaggttcac gatcgccagg tgaccgccca cccagagcat cgccaccgtg ccgaccacgc
      121 tgatcacgcg gaacaccgca ggcatggagc gcgcgatccg cgccccggtg tggcgcaccc
      181 ggcgcaccgg gctcttcgcc atgccgagcc cgatgtcgtc gatcttcacc agcagggcga
      241 cggcgccgta cacgacgccc gtcatgatca gcgcgatgac cgccagcacg ccgagcgtct
      301 gccagatgcc gaggttcttg tccaggctcg ccagcgagat gagcatgatc tcggtggaga
      361 ggatcaggtc ggtgcggatc gctccccaga ccagccggtt ctcgtcgcgc tctccctcat
      421 cgccgtggcc gtggccgaat ccgaaccact ccagcacctt ctcggcgccc tcgaagcaga
      481 ggtacgtgcc gccgacgatg agcaggtacg gaagcacccc cggcgcgaac gcggtgagca
      541 gcagcgcgat ggggatgatc acgatgaact tattcgcgat cgacccgagg gcgatcttgc
      601 cgaccaccgg cagctcgcgc gcgggggtga gcccctgcac atactgcggt gtgacggcgg
      661 cgtcgtcgat gacgacgccg gccgccttgg cggatgcctt gacggcagcg ctgaggatgt
      721 cgtcgacgac ggcgagcagg cctacggaca tgcggtcccc ctggggatcg tgtgggtggc
      781 cggcgggcgt gccggttcga gtggcaaggc taccgggtcg gcggatgctg ctccgtcagc
      841 gccctcggca cgccggggcg ggaggagccg cgggtcagcc gtgcgaccgc cagcagcacc
      901 gcggtcagcc cgatcgtcag cagcagctgc gtgcgggccg cctggtcgat catcgccagc
      961 gcgatgatca cggccagcag caccaggcac gcccaggaca gcaccgggaa gccccacatg
     1021 cgcatcggca tctcctcgcc gtcgcggtcc gcgcgccggc gcagaatgat ctgcgagatg
     1081 gccgtggccg tccagatgac gaggaccgtc gaaccgacga tgttcagcag caggccgagc
     1141 acgacctcgg ggaaggccca gttcagcccg accgtcacga acccgaaggc gacggaggcc
     1201 agcaccgcca cgtacggcac gcccttcgcg ctggtgcgga tcgccgcgag cggcgccagg
     1261 ccccgctcgc ccagcgagta caccatgcgt gaggcgccgt agatgttggc gttcatggcc
     1321 gacaggaggg ccagaaccac gatcgccgac atcagcaggt cgaccccggg caggtgcagg
     1381 gtggcgagga ccgccgagaa cgggccggcc ttcacctctt tctcgtccca cggcagcacg
     1441 gcgacgatca cgaagatcga gcccatgtag aagatcagga tgcgcaccat cacctcgcgc
     1501 acgatgcggc ggatgttgtt cgcggggtcg tccgactcgg cggcggcgat cgccacgacc
     1561 tcggtcccgc cgaaggcgaa cacgacgatg agcagtgcgg ccgcgatgcc ggtgatgccg
     1621 tgcggcgcga agcctccgtg ggcgacgagg ttgccgacgc cgaccgccgg gacgttcggg
     1681 atgagtccga agatggcgca ggcgcccacg atgaggaagg cgatgatcgc gaacaccttg
     1741 atcgccgcga accagaactc gaaccggccg tagttgcgca cgccgaacag gttcaccgcg
     1801 gtcagcgcgg cgacgaacac gagcacccac gcccaggcgg ggaatccggg agtccagccc
     1861 tgcacgatgc cggctgcccc tgtcgcctct gcagcgatca ccacgaccag ctggatccag
     1921 tacagccagc ccaccgcact gccggcgctg cgtcccatgg cgcgctgcgc gaacgagctg
     1981 aaggcgcccg aactgggccg ggcggccacc atctcggcga gcatcgacat caccaggatg
     2041 acgatcgtgc ccgcgaccag gtacgacacg agcacggcgg gacctgcgat gctgatcgcc
     2101 tcgcccgatc ccacgaagag gccggcgccg atcgcgccgc cgaggcccat catcgagatc
     2161 tgccgacgcg tgaggccggg atgcagcccc tgcgtcgtcg tcgtgatcgc gggaacctcg
     2221 gtcatgggga cctcctcgtc cgatggcgcc acgactccga cagccgctcg ctccgaacgg
     2281 ccacattaga acgatgtgcg gtcggaagca atctgcgccg cgctcgacgc gcaccttgcg
     2341 cgtcgaggag catacatgcc cacccgcgct gatcagaatg cgaagcgtgc gggcggttca
     2401 gccggcgtgc caggccgcac ggacggcgcc gagcgcttcg tcgaggctga gtcctgcgga
     2461 tgccgcgacg aaagcccgcg cggcatcgcg caccgcatcg ggatgcgcgt gcccggcgct
     2521 caccctggtg ccgcgggcgc ggctggtctc gacgagaccc tcctcctcga gcagcgcgta
     2581 ggccttcgcg accgtgcctg ctgcgacccg gaggtcgccc gccagctggc ggatcgacgg
     2641 gagacggtgg ccgttctcga gttcaccgga tcggatgcca tcggccagct gcgagcggat
     2701 ctgctcgaac ggcgcgaggg cgccggaggg gtcgaccgtg atcatcgcac cggctccgg
//
LOCUS       DATMSX010000104        11435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_96409,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000104 DATMSX010000000
VERSION     DATMSX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96409"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VN041_03260"
     CDS             <1..679
                     /locus_tag="VN041_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWU28096.1"
                     /translation="AASAPRAGTRPRTKTYGQAGGWRRLRYLQLMTEHLGRTVTAREI
                     YDPAVVREAPWEARRMLADALAEESTAAAIADLGIGFTAWNDVVAGADAAQKLDHAVL
                     GPTGLYGLASEDFGDAVRFRRGEVIGRSVDGSTPVADLLSRIRSLSRAARVRFAGAIL
                     ILPDDDLDEAVTVLGAVRGVPVAVVRRSALRPVLRSGIPGARAMGMTEAFDVRTRLTQ
                     TARVLVP"
     gene            714..1364
                     /locus_tag="VN041_03265"
     CDS             714..1364
                     /locus_tag="VN041_03265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU28097.1"
                     /translation="MTDPLPDRPAPDNPQGKLVLLRHGETEWSRSGRHTGRTDIPLTA
                     HGEELARAAGELVRGYDFTLVLSSPLQRARRTAELAGLDAEIDPLLVEWDYGGYEGRT
                     TRDIRAELGYNWTAFAHGVIRGDTPGETVEEVAARASRVLTRVLPAMADGDVALVAHG
                     HYLRILTAVFLRQAPRFAASISLDAGSASVLGFSREQPSILAWNHGPALPLAPSES"
     gene            complement(1502..3139)
                     /locus_tag="VN041_03270"
     CDS             complement(1502..3139)
                     /locus_tag="VN041_03270"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWU28098.1"
                     /translation="MTMLPRRTPRSAGVDANGIAGFLDEMSKSGIELHSCMIVRRGSV
                     VFEHWWAPYDPQTPHLLYSLSKSFTAVAAGIAEAEGLLKLDDRLSDHLDETAHYGDAT
                     IADALRMSVGHLLDPVLDPTTDSGEMNDVALRAKLRDYSPERKPGEVFTYDQLATFAV
                     AKIIEARSGTSLLEYLRPRLLAPLGAAEAKWYGGEANPGLTGLYLCTESIAAFGQLLL
                     QEGEWGGEQLVPRDWLRRATSTQMYNDSAHRFPPDMPVDRDSGLGYGYQFWIGDHGYH
                     AGGAYGQFCLVLPGSDAVVAVTASTQAGQPVLDAVWEHLLPAFAADVPDDEVTAAADL
                     RLAKRFADATVVPPVAGVFDLAACTAKATLGQLAVVTDALRGGTSSELWHGAAAHASE
                     WTERAVEAIASAAADPVPLVDPICRDVSTLTFTRAARDASLPYTRFGGLGDIDMPELT
                     AVRLSGNVLTLTLEEGLAHLAVEPDRWQRGVLPGEIPIPFTTRGGWAGDGRFEVELRM
                     IEGPHVGYLLLDPETDEFSFIWREPTLAGRGLAGYRA"
     gene            complement(3136..3831)
                     /locus_tag="VN041_03275"
     CDS             complement(3136..3831)
                     /locus_tag="VN041_03275"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWU28099.1"
                     /translation="MLQTPTGSTDTRNTVGVELVTAGLLWILGPVWYLLCEAIAAAAF
                     PGYSYATFYISDLGVPTFGDFQGRPLHSQLPAVMDAGFIGSGLLFVLGLVVIAPRLGR
                     RAATVWFMLLGLIHAMGIVFVGLVPGSPENAASGLIVIHGAGAVAAIGGGNAAAIVSA
                     RAFRAIAVPTWHRRIGLVLGASGWCSAALLTMHVWLPDGVWERGAVYTFLLWQLLSGV
                     VLISNRREGESKR"
     gene            complement(3803..5704)
                     /locus_tag="VN041_03280"
     CDS             complement(3803..5704)
                     /locus_tag="VN041_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU28100.1"
                     /translation="MRGGPSTRTTPDGLAFRDLNGNGIMDPYEDSRLSAEERVNDLLP
                     RLSLREKIGLMFQTVIEMGPDGELLAESGVFAKSATATVVLDKGLSAFNVHAIPSARA
                     AARWNNRLQALAEQTPHGIPVTLSTDPRHSFTESSGVAFTSGPFSQWPDMLGLAALGD
                     VDLVARFSEVVRAEYRAVGLRMALHPQVDLATEARWGRQLQTFGSDVDRVRAYTRAYV
                     RGIQGPRLGPDSVATTTKHFPGGGAQKDGEDPHFPYGREQVYPGNNFEAHLAPFADAI
                     AAGTAAIMPYYGMPVGLVRHGRPIEEVGFAYNRQILTGVLREELGFDGVIVSDWELVT
                     DNHVGGKLLPARAWGVEALAPAERIVKLLHAGVDQFGGEECVDVLADAVAQGAVTESR
                     IDESARRILLTKFRLGLFDDPYVDEDAAERIVGNAEFRAQGLKAQAQSIVVLTNHDVL
                     PLGGRRSPLRIYVEGVMPEIVAEYGEPVAAPRDADLALVRIPAPYEPRDDLLLERGFH
                     QGSLDFRPGLVSRLARIAEECPLVLVVDLDRPAILAPFLSFASATVACFGVADSALMD
                     GLSGRVVPSGRLPVDIPRSMDDVRKAMPDRAAPAADALFARGFGLQDYGRRQEEVHSA
                     DASDADRFD"
     gene            complement(5733..6473)
                     /locus_tag="VN041_03285"
     CDS             complement(5733..6473)
                     /locus_tag="VN041_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28101.1"
                     /translation="MLLGSFKTQGEILRRPPTFWPEQFTGQNYTTWFAELDFSKYFLN
                     SVIVAVITVLGNLVFCSMVGYALAKMSFFGKRLLFGLVMLTLMVPGVVTFVPLFVQTA
                     KLGLLDTYAALVLPFLTAPVGVFLMRQFISGIPDELLEAARIDGANEWRLFSRVVLPL
                     CGPALATLAILTFLGSWNNFLWPLVVSQSADLYTLPIALSLYSTGQHSTNYGLLLAGS
                     VLVIAPILLLFVVLQRWFVQGIATTGLK"
     gene            complement(6623..7570)
                     /locus_tag="VN041_03290"
     CDS             complement(6623..7570)
                     /locus_tag="VN041_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28102.1"
                     /translation="MTASSIVRRASLRRGTTSGSHRRTLTRRRQTIFAWTFCLPFVAV
                     FAAFMLMPIGGSFAMSFTDITARDLQTPFAVAFVGIDQYVSLLTDPRFLTALSVTGMF
                     VVVGIPLTMAVALALAVGLNTGQGRIITILRVGYYAPAVTSIVAVSVAWKYILQPDGL
                     LNAALGVFGLPAVNWLNDPVWALPSLIMLALWRNVGTLMVIFLAGLQSIPREMFEAAT
                     MDGAGAWRRFFSVTLPTLRPTLLLGAVLISVGYLQFFEEAFVMTGGGPLDRTLSIAFY
                     TYQQFGVGLYGRASAASYILFLLVALLSLLQFRLLRTKE"
     gene            complement(7571..8839)
                     /locus_tag="VN041_03295"
     CDS             complement(7571..8839)
                     /locus_tag="VN041_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28103.1"
                     /translation="MKRQARVLATATAIVLAVAALTGCGRAATGTASTAPASKVGDGP
                     ATGTVSMWTMGSEADAVKPLIAEFEKLNPDVKVNLTAVPWAAAHSKLQTAIAGGATPD
                     VSMIGSSWMSEFAPALATVPDGFSGEDFFPGVLEGTKVDGRWAAVPWYADIPMLYYRT
                     DLAQKAGWDHAPETWDEVQQLAADYQGKAGATWGMRLYSGPSSFLGNLWIPFTGGAKL
                     EDGKKWTFTTPEWQHSLEFYKSWFDKRLSDPNSDPAPAQMPADFISGSTPMFVATPSL
                     QGTLAQMGGTDFESKYALAPLPYGEAQVSVVGGSQLGVFEKAKNSDAAWKLVRWLSTP
                     ETQAAFFEATGDLPPGPAAWELPALTGKDRIAPVGEALKTALPLPTPPTLDQVFAIGD
                     STLEQIRKNTVSIPAGLQSLQRQADQVGLG"
     gene            complement(8979..10094)
                     /locus_tag="VN041_03300"
     CDS             complement(8979..10094)
                     /locus_tag="VN041_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28104.1"
                     /translation="MAEKRPTVYDVAERAGVSIATVSHSFRRPERIRPETRELVLTAA
                     RELGYVPSGAARSLAHGSTGALGLHLFDLLLPQRGVDVPGTGSDTELLSSIVSAARAE
                     SAPAWFAAADTADHDPRVFPLYVDEIQRGFVIECRDRDRPVVLSQGSTDQSDVANTVG
                     RVDGLAIFPGRSSADQLIDILGDRPVVLFSSRPDPRTAQVNSDNRSGMRQIVEHLATV
                     HGARSLGWVGETAGQFDFAERFQAFTDAVAAHGDLDMRVIDAHSLDDDPTLRGVEDAV
                     RSGDLPDAFVCGSDQSALVVLDTLQAAGVRVPQDVRVTGFDDVLAAQLCRPGLTTVRQ
                     QFELMGRVAAHLLISEPAAKLTVTLGVSLTVRGSCGC"
     gene            10298..11122
                     /locus_tag="VN041_03305"
     CDS             10298..11122
                     /locus_tag="VN041_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28105.1"
                     /translation="MKTTEGSRDIATLHRPVGIFDAGLGSYDLVRRIRAAYPRQDVVY
                     LADRASFPYGAKSEEALLSSVERAALALESLGASSIILASNAPSVTVLEPLRPRVSVP
                     ILGVIPPVRAALAAISHDHTLAVAGASVLARSDALRQLISDAAGDQAHRVHAVAADAL
                     IGLVESGAFLDRAKIRRPIAGFLAALHERSPQVGGLSLSSTHLPWLAAAIAEQAPDLT
                     LFDPADDVVAEFAPLTTRGSGRLVSLATASRSHPIDEFHSMLQRLRLDITPALIEL"
BASE COUNT         1929 a         3854 c         3865 g         1787 t
ORIGIN      
        1 tgcggcatcc gctccccggg ccgggacgcg gccgcgcacc aagacgtacg gtcaggcggg
       61 gggctggcgg cggctgcgct acctgcagct gatgaccgag cacctcggtc gcacggtcac
      121 ggcgcgcgag atctacgatc cggccgtcgt gcgcgaggcg ccgtgggagg cgcggaggat
      181 gctggccgac gccctcgccg aggagagcac ggccgccgcg atcgccgacc tgggcatcgg
      241 gttcaccgcg tggaacgacg tggtggcggg agcggatgcg gcgcagaagc tcgaccacgc
      301 ggtgctcgga cccacgggtc tgtacgggct ggcgtccgag gatttcggcg acgccgtgcg
      361 gttccggcgg ggcgaggtga tcgggcggtc tgtcgacggc agcacaccgg tggcggatct
      421 gctctcccgc atccgttcgc tctcccgcgc cgcgcgggtg cggttcgccg gcgcgatcct
      481 gatcctgccc gacgacgacc tcgacgaggc ggtcaccgtg ctgggcgccg tgcgcggcgt
      541 tcccgtcgcg gtcgtgcgcc gcagcgcgtt gcgccctgtg ctgcggagcg gcatccctgg
      601 agcgcgcgcg atgggcatga ccgaggcctt cgacgtgcgg acgcgcctga cgcagaccgc
      661 gcgcgtgctc gttccctgag agcgaccggc ggtacggttc aggggtggat gctgtgactg
      721 acccgctgcc cgaccgccct gcgccggaca acccgcaggg caagctcgtg ctgctgcggc
      781 acggcgagac cgagtggtcg aggtcgggac ggcacaccgg ccggaccgac atcccgctca
      841 ccgcacacgg cgaggaactg gcccgcgcgg cgggcgaact ggtgcgcggg tacgatttca
      901 cgctcgtgct gagctccccg ttgcagcgcg cacgacgcac cgccgagctg gccgggctgg
      961 acgccgagat cgacccgctg ctggtggagt gggactacgg cgggtacgag ggacgcacga
     1021 ccagggacat ccgcgcggag ctcggctaca actggacggc cttcgcccac ggggtgatcc
     1081 gcggcgatac ccccggggag accgtcgaag aggtcgccgc ccgcgcctcg cgggtgctca
     1141 cccgggtgct gcctgccatg gccgacgggg acgtcgccct ggtcgcgcac ggccactacc
     1201 tgcgcatcct gaccgccgtg ttcctgcgcc aggcgccgcg gttcgcggca tccatctcgc
     1261 tggacgcggg ctcggcgtcg gtgctcggct tctcccggga gcagccgtcg atcctcgcct
     1321 ggaaccacgg tccggccctg ccgctggccc cgtcggagtc ctgagccggg gagcggatgc
     1381 cggtcgacca tcggcatccg ctccgctgag gtcgtcacgg gtcctgcctg ctccgggcac
     1441 tctcacctcg ggacttgagg cgatccgcag cgtcggcgat ggtgatccgg ccggcatcag
     1501 gtcatgcgcg atagcccgca agccctcttc ccgcaagagt cggctcacgc cagatgaagg
     1561 agaactcgtc cgtttcgggg tcgagcagga gatagccgac gtgaggcccc tcgatcatgc
     1621 ggagctcgac ttcgaacctg ccgtcgccgg cccagccgcc ccgcgtcgtg aacggtatcg
     1681 ggatctcccc gggaaggacc ccgcgctgcc atcgatcggg ttccaccgcg aggtgtgcga
     1741 gcccttcctc cagagtcaga gtgaggacgt ttccgctgag ccgtaccgcc gtcagttctg
     1801 gcatgtcgat gtcgccaagg ccgccgaacc gcgtatacgg aagggatgcg tcgcgtgccg
     1861 cgcgcgtgaa cgtcagcgtc gagacgtccc ggcagatggg atcgacgaga ggcacgggat
     1921 cagcggcagc cgacgcgatg gcctccaccg cgcgctccgt ccattcactc gcgtgtgctg
     1981 cggccccgtg ccacaactcg gacgaagtgc cgccgcggag ggcatctgtg acaaccgcca
     2041 gctggcccag ggtcgccttc gcggtgcatg ctgcgagatc gaacacaccg gcgacaggcg
     2101 gcacgaccgt ggcatccgcg aatcgcttcg cgaggcggag gtccgccgcc gcagtcacct
     2161 catcgtcggg gacgtccgcg gcgaaggccg gcagcagatg ttcccagacg gcgtcgagca
     2221 cgggctgtcc tgcttgtgtc gacgcggtca cagcgacgac tgcgtcgctg ccgggcagca
     2281 cgaggcagaa ctgaccgtac gcaccgccgg cgtgatatcc gtgatcgccg atccagaact
     2341 ggtacccgta accgaggccg ctgtcacgat cgacgggcat gtccggaggg aagcgatggg
     2401 cactgtcgtt gtacatctgc gtggacgtcg cgcgcctcag ccaatcgcgc ggaacgagtt
     2461 gctctccacc ccactcccct tcctgcagca ggagctgccc gaaggcggcg atggattcgg
     2521 tgcagaggta aagaccggtg agaccggggt tggcctcgcc cccgtaccac ttggcttcgg
     2581 cagcgccgag aggtgccagg agccgcggcc gcaggtactc cagcaacgag gtgccgcttc
     2641 gtgcctcgat gatcttcgcc accgcgaagg tcgccagctg gtcgtatgtg aacacctcgc
     2701 cgggcttccg ttccggcgaa tagtcgcgca atttcgcccg cagagcgacg tcgttcatct
     2761 caccgctgtc tgtcgtcggg tcgagaacag ggtccagcag gtgtcccacc gacatgcgca
     2821 acgcatccgc gatcgtggcg tcgccgtagt gggcggtctc gtcgagatgg tccgacaacc
     2881 ggtcatcaag cttcagcagt ccctcggcct cggcgatgcc ggcagcgacc gcagtgaaac
     2941 tcttgctcag tgagtagagc aggtgggggg tctgcgggtc gtacggagcc caccagtgtt
     3001 cgaagaccac cgacccgcgt ctgacgatca tgcagctgtg caactcgatg ccgctcttcg
     3061 acatctcgtc gaggaagcct gcgatcccgt tcgcatcgac gcccgcggag cggggtgtgc
     3121 gtctcggcaa catcgtcatc tcttcgactc cccttcacgc cggttactga tcaggacgac
     3181 tccgctcagg agctgccaga gaaggaacgt gtacacggcc ccacgctccc agactccgtc
     3241 ggggagccag acatgcatgg tgaggagggc tgcgctgcac cagcccgacg ctccgaggac
     3301 gagcccgatc cggcggtgcc aggtgggcac ggcgatcgcg cgaaaggcgc gggcggaaac
     3361 gatcgcggca gcgttgcctc ctccgatcgc cgccacggcc cccgctccgt ggatgacgat
     3421 gagacccgat gcggcgttct ccggcgaacc gggcacgagc ccgacgaaca cgatgcccat
     3481 cgcgtggatc agcccgagga gcatgaacca gacggtggcg gcgcgacgac cgagtctcgg
     3541 cgcgatgacg accaacccga gcacgaacag caagccggat ccgatgaacc cggcatccat
     3601 caccgctggg agctgggagt gcagcgggcg accctggaaa tcgccgaagg tcggcacgcc
     3661 gagatcgctg atgtagaagg tcgcgtaact gtagcccggg aacgcagccg cagcgatcgc
     3721 ctcgcacagc aggtaccaga ccggccccag aatccagagc agcccggccg tcacgagctc
     3781 gaccccgacc gtgtttcgcg tgtcagtcga accggtcggc gtctgaagca tctgcactgt
     3841 gcacctcctc ctggcggcgg ccgtagtcct gtagcccgaa gccccgcgcg aacaacgcgt
     3901 ccgcagccgg ggccgcacgg tccggcatgg cctttcgcac atcatccatg cttcggggga
     3961 tgtcgacggg gaggcggccc gacggcacga cccggccgga caggccatcc atgagcgcgc
     4021 tgtcggcgac gccgaagcat gccacggtcg cactcgcgaa cgagaggaac ggagcaagga
     4081 tcgcgggccg gtcgaggtcg accacgagca ccagcgggca ctcctcggcg atcctggcga
     4141 gtcgggagac gagtccgggc cggaagtcga gggatccctg gtggaatccg cgctcgagca
     4201 ggaggtcgtc acgtggttcg tatggagcgg ggatgcggac gagcgcgaga tcggcgtccc
     4261 gcggagccgc caccggctct ccatactccg ccacgatctc gggcatcacg ccttcgacgt
     4321 agatgcgcag cggagaacgc cgcccgccga gcggcaagac gtcatggttc gtgagaacga
     4381 cgatcgactg cgcctgtgcc ttcaagccct gagcgcggaa ctcggcgttg ccgacgatcc
     4441 gctccgccgc gtcctcgtcg acatacgggt cgtcgaagag cccgagtcgg aatttcgtga
     4501 ggaggatgcg gcgcgcggac tcatcgatcc tgctctccgt gaccgcccct tgagcgacgg
     4561 cgtcggcgag gacgtccacg cactcctctc ctccgaattg atcgactccg gcatggagaa
     4621 gcttcacgat gcgttccgcc ggggcgagcg cttcgacccc ccacgcgcgg gccggcagca
     4681 gcttgccacc gacgtgattg tcggtcacga gctcccaatc ggagacgatg acgccgtcga
     4741 acccgagttc ctcgcgcagc acacccgtca ggatctgcct gttgtatgcg aagccgacct
     4801 cttcaatggg ccggccatgt cgcacgaggc cgaccggcat cccgtagtag ggcatgatcg
     4861 ctgcagttcc ggcggctatg gcgtcggcga agggggcaag gtgcgcctcg aagttgttgc
     4921 cgggatagac ctgctcccgt ccgtacggga agtgcggatc ctcgccatcc ttctgcgctc
     4981 cgccgccagg aaagtgcttc gtcgtggtcg ccacgctgtc cggcccgagg cgaggaccct
     5041 ggattcctcg cacataggcg cgggtgtagg ccctcacccg gtcgacatcg ctgccgaagg
     5101 tctgcagttg gcgcccccat cgggcttcgg tcgcgaggtc gacctgcgga tgcagcgcca
     5161 tgcgcaggcc gacggctcgg tactcggcgc gcaccacctc gctgaatcgc gcgacgaggt
     5221 cgacatcgcc cagcgccgcg agccccagca tgtcgggcca ttgcgagaac gggccgctcg
     5281 tgaacgcgac gccactgctc tcggtgaagg agtgtcgtgg atccgtgctc agtgtgacgg
     5341 ggatcccgtg cggcgtctgc tctgcgaggg cctggaggcg attgttccac cgtgctgcgg
     5401 cacgcgcgga cgggatcgca tggacgttga aggccgacag gcccttgtcc agcaccaccg
     5461 tagccgtagc ggacttcgcg aagacaccgg attccgcgag aagctcgcca tcaggaccca
     5521 tctcgatgac ggtctggaac atcagaccga tcttctcccg cagggagagc ctgggcagga
     5581 ggtcgttcac ccgctcttcc gcactgagcc gcgaatcctc gtaggggtcc atgatgccgt
     5641 tgccgttgag gtcgcggaac gccaacccgt ccggcgtcgt gcgggtgctc ggtccgcccc
     5701 tcatgtcgtg tcgattcact cttcttctcg gttcacttga gcccggtcgt ggcgatgccc
     5761 tggacgaacc atcgctgcag cacgacgaac agcagcagga tcggcgcgat caccaagacg
     5821 gaaccggcga gcaacagacc gtagttcgtc gagtgctgac cggtggagta cagggaaagg
     5881 gcgatgggca gcgtgtacag gtcggcggat tgggagacga ccagaggcca gaggaagttg
     5941 ttccacgatc cgaggaaggt gaggatcgcg agcgtcgcca gcgccggtcc gcacagcggg
     6001 agcacgaccc gcgagaacag cctccattca ttcgcaccgt cgatgcgggc ggcttcgagg
     6061 agttcatcag gaatcccgga gatgaactgc cgcatcagga acacgccgac gggcgcagtg
     6121 aggaatggca gcacgagagc ggcgtacgtg tccaacaggc cgagcttcgc tgtctgcacg
     6181 aacagcggga cgaacgtgac cacgcccggc accatcaggg tcagcatcac cagcccgaac
     6241 agcagacgct taccgaagaa gctcatcttc gcgagggcgt acccgaccat cgagcagaac
     6301 acgaggttgc ccagcacggt gatcactgcg acgatcacgc tgttgaggaa gtacttggag
     6361 aaatcgagtt cggcgaacca ggtcgtgtag ttctgtccgg tgaactgctc cggccagaag
     6421 gtcggaggtc gacgcaggat ctcgccctgc gtcttgaacg acccgagaag catccagacg
     6481 aacgggagta tccacacgag aagtccgagc acgagcacgc ccgcgaccac gcggcggcca
     6541 cggtggactt ggggaggacg caggacccgc gcgatcttgt ctttgctcgt tgttcgttcc
     6601 gacgtgcgag agaccatcgg tttcactcct tcgtgcgcag cagccggaac tgcagcaggc
     6661 tgagcagggc gaccagcagg aagaggatgt agctcgcggc ggacgcccgc ccgtacagcc
     6721 ccacgccgaa ctgctgatac gtgtagaacg cgatcgagag cgtgcgatcc aacgggccgc
     6781 cgccggtcat gacgaacgcc tcctcgaaga actggaggta tccgacggag atgagcacgg
     6841 ctccgagcag gagggtgggg cgcagcgtcg gcagcgtgac gctgaagaag cgacgccagg
     6901 cgccggcgcc gtccatggtc gccgcctcga acatctcgcg ggggatcgac tgcagccccg
     6961 ccaggaagat caccatgagc gtgcccacgt tgcgccacag cgcgagcatg atcagggacg
     7021 gcagggccca cacgggatcg ttgagccagt tgaccgcagg gaggccgaac actccgagcg
     7081 cggcattgag cagcccgtcc ggctggagga tgtacttcca tgcgaccgac accgcgacga
     7141 tgctggtgac ggccggggcg tagtagccga cgcgcaggat cgtgatgatc cttccctgac
     7201 ctgtgttgag gccgaccgcc agcgcgagtg cgacagccat ggtcagggga atgccgacca
     7261 ccacgaacat tccggtcacc gacagggccg tgaggaatcg cgggtccgtc agcagcgaca
     7321 catactggtc gatgccgacg aacgccacgg cgaacggcgt ctgcaggtcg cgcgcggtga
     7381 tgtcggtgaa cgacatcgcg aaggatccgc cgatcggcat gagcatgaac gccgcgaaca
     7441 ctgcgacgaa cgggagacag aaggtccagg cgaagatcgt ctgccggcgt cgcgtcaggg
     7501 tgcggcggtg gctgcccgac gtcgtgcccc gccgaagcga ggcacgacgc acgatggaag
     7561 aggctgtcat ctagccgagc ccgacctggt cggcctgccg ttgcaggctc tgcagaccgg
     7621 cggggatcga gaccgtgttc tttcggatct gctcgagggt gctgtcgccg atcgcgaaga
     7681 cctgatcgag ggtcggcggg gtcgggaggg gcagggccgt cttgagcgct tctccgacgg
     7741 gggcgatgcg gtccttgccg gtcaaagcgg gcagttccca cgcggccggg cccggtggca
     7801 gatcccccgt cgcctcgaag aaggcggcct gggtctcggg cgtgctcagc caacggacga
     7861 gcttccaggc cgcgtcggag ttcttcgcct tctcgaagac gcccagttgc gagccgccga
     7921 ccacggagac ctgcgcctca ccatacggga ggggggcgag ggcgtacttg ctctcgaagt
     7981 cggtgccacc catctgcgcg agagtgccct gcagcgaggg cgtcgccacg aacatgggtg
     8041 tactgcccga gatgaagtcc gccggcatct gcgccggtgc cggatcagag ttgggatcgg
     8101 agagtcgctt gtcgaaccag ctcttgtaga actcgagcga gtgctgccac tccggggtgg
     8161 tgaaggtcca cttcttgccg tcctcgagct tcgcaccccc ggtgaacgga atccacaggt
     8221 tgccgaggaa ggaactcggc ccgctgtaga ggcgcattcc ccacgtcgcg cccgctttgc
     8281 cctgatagtc cgcggcaagc tgctgcacct cgtcccaggt ctccggtgcg tggtcccatc
     8341 cagccttctg cgccaggtcc gtccggtagt acagcatcgg aatgtccgcg taccaaggca
     8401 cagcggccca ccggccgtcg acttttgtgc cctcgagcac accggggaag aagtcctccc
     8461 ccgagaagcc gtcggggacc gtcgccagcg caggggcgaa ctccgacatc cagctcgagc
     8521 cgatcattga gacgtccggc gtcgcgccgc cggcgatcgc ggtctgcagc ttcgagtgcg
     8581 ccgccgccca tggcacggcg gtgaggttca ccttgacgtc ggggttgagc ttttcgaatt
     8641 ccgcgatgag cggcttcact gcgtcggcct ccgatcccat cgtccacatg gagacggtgc
     8701 ccgtggccgg accatctccg accttggatg cgggggcggt cgaagcggtg cctgtcgcag
     8761 cccttccgca tccggtgagc gccgcgaccg cgaggacgat cgcagttgca gtggcgagca
     8821 cgcgtgcctg tcttttcaca actcctcctt gagtggaaag gtcgcccttg tgacgactgt
     8881 caagacttgg ttatgcgcat aaccttacgc ggatgtacgc gcataaccaa gagcctttct
     8941 ggaggcggac cggcgggagt ctgccgcgca gtgggctctc agcagccgca gctgccgcgc
     9001 accgtcagcg agacgccgag cgtcacggtc agctttgcgg caggctcact gatgagcagg
     9061 tgggcggcga cgcgacccat gagttcgaat tgctggcgca ccgtcgtgag gcccggccgg
     9121 cagagctgag ccgcgagcac atcgtcgaac ccggtcaccc gcacgtcttg cggcactcga
     9181 acccccgccg cctgcaacgt gtccagcacg acgagtgcag actggtcact gccgcagacg
     9241 aaggcgtcag gaagatcacc gcttcgcact gcgtcttcca ctcctcgtag ggtcggatcg
     9301 tcgtcaaggc tgtgcgcgtc gatgacacgc atatcgaggt cgccgtgcgc cgcaacggca
     9361 tccgtgaacg cttggaacct ctcggcgaag tcgaactgcc ctgccgtctc tcccacccag
     9421 cccagcgaac gcgctccatg cacggtggcc aggtgctcca cgatctggcg catgccactg
     9481 cggttgtcgg agttgacctg agctgtgcga gggtccggcc tgctgctgaa gagcacgacg
     9541 ggacggtcgc cgaggatgtc gatgagctgg tctgcgctgg agcggccggg gaagatcgcc
     9601 agtccgtcga cgcggccgac ggtgttcgcg acatcggact gatccgtgct tccctgactg
     9661 agcaccacgg gccggtcgcg gtcacggcac tcgatcacga aaccgcgttg gatctcatcc
     9721 acgtacagcg ggaacaccct cggatcatgg tccgccgtgt ccgccgcggc gaaccaggcc
     9781 ggcgcggact ccgcccgcgc cgcgctgacg atcgacgaga gcagttcggt gtcagagccg
     9841 gtacccggca cgtccacccc gcgttgcgga agcagcaggt cgaacagatg cagtcccaga
     9901 gcacccgtgg atccgtgagc gagcgagcgg gcggcgccgc ttggcacgta cccgagttcg
     9961 cgggccgcgg tgagcacgag ctcccgggtc tccggacgga tgcgctcggg tcggcggaat
    10021 gagtgcgaga ccgtcgcgat ggacacacct gctcgctcag ccacgtcata caccgtcggg
    10081 cgcttctcgg ccatgtcgag cctttctggg aatcgagccg cgacccgatc ctgtgaagtc
    10141 tattcgccga ctgcatccgg ctttgcgtca gtgctcctgc caggaagaac actctccgca
    10201 cagacgatcc gtgggcgctc accatgtgac gtagccgagc cagggggatc agcgccggcc
    10261 ggcctcgcct ccaagattgc ggactagagt ctctcgcgtg aagacaaccg agggttcccg
    10321 cgacatcgcc acactccacc gcccggtcgg aatcttcgat gcaggactcg gcagctacga
    10381 cctcgtgcgc cgcatacgcg cggcttatcc tcggcaagac gtggtgtacc tcgccgaccg
    10441 cgcgagcttc ccttacggcg cgaaaagcga agaggcactt ctgtccagcg tcgagcgggc
    10501 tgccctggcg ctcgagtctc ttggcgcgag ctcgatcatc ctcgcctcca acgcgccgag
    10561 cgtcaccgtt ctcgaaccgc tgcgccctcg ggtgagcgtg ccgatcctcg gggtgatccc
    10621 acccgtgcgc gcggcgttgg ctgcgatctc ccacgatcac acgctcgccg tcgccggcgc
    10681 gagtgtactc gcccgcagcg acgcgctgcg acagctcatc tccgatgccg ccggcgacca
    10741 ggcgcaccgc gtacatgccg tggccgccga cgcactcatc ggcctcgtcg aatccggggc
    10801 gttcctcgac agggcgaaga tccggcggcc tatcgccgga ttcctcgccg ccctccacga
    10861 gcgaagcccg caggtcggcg gcctgtccct gtcgagcact cacctgccgt ggctcgctgc
    10921 cgcaatcgcc gagcaggccc ccgatctcac gctcttcgat ccagccgacg acgtcgtcgc
    10981 cgagttcgcc cctttgacca cgcgcggctc cgggcgtctc gtctccctcg cgactgcgtc
    11041 cagaagtcat cccatcgacg agttccattc gatgctgcag agactgcgcc tcgacatcac
    11101 gcccgcgctc atcgagctct gacgcgtctc cggctcgacg ctgcccaggg ccacggatca
    11161 cctgtccaag gggcggagtc ctcgatcggc gcctgtccgt ggcggggttc caggcggcgc
    11221 gcagatagag gggtgccgcg tgtccgaggt cgatggttgg atgacgtcat ggagatcacg
    11281 acctcagcag acggaaccgc catcgccttc accgccatcg gtcagggacg cacgatcgtg
    11341 atcgtgaacg gcgcgctgtc gacggccgcc gacgccgcac cgctggcgca cgcgctgtcg
    11401 gatgccggct tccgcgccgt cacgtgggat cgccg
//
LOCUS       DATMSX010000105         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39532,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000105 DATMSX010000000
VERSION     DATMSX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39532"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VN041_03310"
     CDS             complement(<1..1061)
                     /locus_tag="VN041_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU28106.1"
                     /translation="MRTIRSLLPLIVGAVIVVVLAVLPLLNLSIPGILPGATYTPGSL
                     ALMSLCMVFAALALSYNLMLGTAGMLSFGHALYFGAGAYGLGIVLQAAQLPLVPAVFA
                     ALLGGVVIAVVTGAVAMRVGGIPFAMVTLAFAQAGSVLVRRNQAVTGGEEGLSLNTAG
                     VPDWLVGVINTRNLYWVSLAVVVIVYLIVLWVDRSRLGHLAAAARENDLRVRVLGLQP
                     NRAKLIVFVIAALCASLAGIAYLLLQSGTQPSAVGADLTITVLVMVVLGGVGFRWGAI
                     VGGVVYTILDQRLTVLARSDAIHQLPDILRVPLSEPLFILGVLFILVVMFLPGGIAGT
                     VDGWLRRRRGRAAPDTGITA"
     gene            complement(1064..1939)
                     /locus_tag="VN041_03315"
     CDS             complement(1064..1939)
                     /locus_tag="VN041_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU28107.1"
                     /translation="MSTLVLTLIIGLGLGALYFLVASGLSLIYGLMHVLNFAHGAFLT
                     LSAFVGWAVARAIGVSSWGGFLLSILAGALVGAVFAALTELILIRPLYERHIEQVLVT
                     VGLSFAAIALFEGIWGTDPITVTGPAWLGQITSVLGASIPNKYWVLIVAAGIVLAGLV
                     LFLKKTRYGMIIRAGVENRAMVTALGIDVRRSFTLVFAIGGAAAGIGGVLAMHAMTYV
                     SAHLGSTLLIFAFIVTVVGGLGSLTGAAVASVLVAVLQQVANTYLGGTGDFIVVILLA
                     VVLLVRPAGLMGKRA"
     gene            complement(1936..2646)
                     /locus_tag="VN041_03320"
     CDS             complement(1936..2646)
                     /locus_tag="VN041_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28108.1"
                     /translation="MSGPILQVRNLRASIAGQQVVEDVSFDVPATGITAVLGRNGAGK
                     TSTLRGILGLISRRGEVLLDGERIDALSTHRIVQRGVGYVPEDREVFAGLTVAENLAL
                     AERQREPRRDFVAQLFPDLVARREQRAGTLSGGQQQMVSVARALLNDNRLLLVDEPTK
                     GLAPKIVTEVADALAEAATTVPMLLVEQNLEVVRSLADQAIVIADGRVVHTGTASEIL
                     DDANLTRRLLGVSAEEHA"
     gene            complement(2643..3419)
                     /locus_tag="VN041_03325"
     CDS             complement(2643..3419)
                     /locus_tag="VN041_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28109.1"
                     /translation="MTTPAPPASGASLRVEGLGLEIGGATILKGVDLAVEPGALVGVI
                     GPNGAGKTTLFNTISGVIRPTAGRILLDGADITRTSVPQRARAGLGRTFQTSSLFPQL
                     SVRENVRMAVQATDNGSYSLFHFPRRSDAASARAEELLRSVGLGHRLDARAGDISHGD
                     KRKLEIAVLLATRSRLVLLDEPMAGVASGDVAGLVDSIRGLQAETGCTVLMVEHHIEV
                     LMGLVDVVAVMYFGSIIAVDTPQRIMENATVQSAYLGTGS"
     gene            complement(3423..>4520)
                     /locus_tag="VN041_03330"
     CDS             complement(3423..>4520)
                     /locus_tag="VN041_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU28110.1"
                     /translation="GGDKGPAEVRVGVITSETGPLAGYGKQYLDAFAAGLDYATDGKG
                     AVGGTKIVIEHRDDAGDPDTAVTAAKELIGDGVNILMGSASSGVALAMAEQAAHNKVL
                     FISGPAAADAITGVNEYTFRSGRQSAQDVATAGTFLKDIKGKKISVFAQNNAFGQGNV
                     AAVQAILGAKGATVEPVLVAEDVTEFTPFAQQVLASKPDLVFVAWAGATSGAMWQAMS
                     QQGVLDAIPVVTGLGDRATFGAYGEASEKINFLNHYFGGAADNKVDTAMVEALKKKGA
                     EPDLFSPDGFNAAIMLVHAVKEGKGEVNAMIKALEGFTFQGPKGENTVRAKDHALIQD
                     MYQAKLVASGGTFTPELVDTVPATDVAPAEK"
BASE COUNT          731 a         1542 c         1581 g          666 t
ORIGIN      
        1 gccgtgatgc ccgtgtcggg cgccgccctg ccccggcggc ggcgcagcca gccgtcgacg
       61 gtcccggcga tgccgccggg gaggaacatg accacgagga tgaacagcac gcccagaatg
      121 aacagcggct ccgacaaggg cacacgcagg atgtcgggga gctggtggat ggcatcggac
      181 ctggccagca ccgtgagacg ctgatcgagg atcgtgtaga ccactccgcc cacgatcgcg
      241 ccccagcgga atccgactcc gcccagcacc accatcacca gcacggtgat cgtgaggtcg
      301 gctccgacgg cgctgggctg tgtgcccgac tgcagcagca ggtacgcgat acctgcgagg
      361 gaggcgcaca gagcggcgat cacgaagacg atcagcttcg cccgattcgg ctgcagtccc
      421 agcacccgca cgcgcaggtc gttctcgcgt gcggcggcgg ccaggtgtcc cagccgcgaa
      481 cggtccaccc agagcacgat caggtagacg atcaccacga ccgccagcga cacccagtac
      541 aggttgcggg tgttgatgac gcccaccagc cagtccggaa cccctgcggt gttcaggctc
      601 aggccctcct caccgccggt gaccgcctgg ttgcggcgga cgagcacgga gcccgcttgt
      661 gcgaaggcga gtgtcaccat cgcgaacggg atcccgccca cgcgcatggc cacggctccg
      721 gtgaccacgg cgatcacgac gccgccgagc agcgccgcga acaccgccgg cacgagcggc
      781 agctgagcgg cctggagcac gatgcccagt ccgtaggcgc ccgcaccgaa gtacagagcg
      841 tgcccgaagg acagcattcc tgccgtgccg agcatgagat tgtacgacag tgcgagggcc
      901 gcgaacacca tgcacagcga catcagggcg agcgagcccg gcgtgtaggt cgccccgggc
      961 aggatgccgg ggatcgagag gttcagcagc gggaggaccg cgagcacgac aacgatcacg
     1021 gcgccgacga tgagcgggag gaggcttcgg atggtccgca tggtcatgcc ctcttcccca
     1081 tcaggccggc cggtcgcacc agcagcacga cagccagcag gatcacgacg atgaagtcgc
     1141 ccgtgccgcc gaggtaggtg ttggccacct gctgaagcac ggccacgagc accgaggcga
     1201 cggcggcgcc ggtcagggag ccgaggcccc cgacgacggt gacgatgaac gcgaagatca
     1261 gcagcgtgga gccgaggtgc gccgagacgt aggtcatggc gtgcatggcg agcaccccgc
     1321 cgatgccggc ggccgcgccg ccgatcgcga agaccagggt gaacgagcgg cgcacgtcga
     1381 taccgagggc ggtgaccatg gcccggttct cgacgccggc gcggatgatc atgccgtagc
     1441 gggtcttctt caggaacagc acgagcccgg cgagcacgat tcccgcggcg acgatgagca
     1501 cccagtactt gttcgggatg ctggcgccga gcaccgaagt gatctgcccg agccacgccg
     1561 gccctgtgac cgtgatggga tccgtgcccc agatgccctc gaacagcgcg atcgcggcga
     1621 acgagagccc gaccgtgacc agcacctgct cgatgtgccg ttcgtacagc ggccggatga
     1681 ggatcagctc cgtcagggct gcgaacaccg cgcccaccag agcgccggcg aggatcgaga
     1741 gcaggaagcc gccccagctg gagacgccga tggcccgggc gaccgcccag ccgacgaagg
     1801 cgctgagggt gaggaacgcg ccgtgcgcga agttcagcac gtgcatcagg ccgtagatga
     1861 ggctcagccc gctcgcgacc aggaagtaca gcgccccgag gcccaggccg atgatcaggg
     1921 tgaggacaag cgtgctcatg cgtgctcctc cgcactgacg cccagcaggc gtcgggtcag
     1981 gttcgcgtcg tcgaggatct cggatgctgt tccggtgtgc accacgcggc cgtccgctat
     2041 cacgatggcc tggtcggcca ggctgcgtac gacctcgagg ttctgctcca cgagcagcat
     2101 gggaacggtc gtggcggcct cggcgagggc atccgccacc tcggtgacga tcttgggcgc
     2161 cagtcccttg gtgggctcgt cgaccagcag cagcctgttg tcgttcagca gggcgcgggc
     2221 gaccgacacc atctgctgct gtcctccgga gagcgtcccg gcgcgctgct cacggcgcgc
     2281 gaccagatcg gggaacagct gagccacgaa gtcccggcgc ggctcacgct gacgctcggc
     2341 gagcgcgagg ttctcggcga cggtgagccc ggcgaacacc tcgcgatcct cggggacgta
     2401 gccgacgccg cgctgcacga tgcggtgcgt gctgagtgca tcgatgcgct ctccgtcgag
     2461 gagcacctcg cccctgcggg agatgagtcc gaggatgccg cgcagcgtcg aggtcttgcc
     2521 cgcgccgttg cgcccgagca cggcggtgat gccggtggcg ggcacatcga aggacacgtc
     2581 ctcgaccacc tgctggccgg cgatggacgc gcgcaggttc cgcacctgca ggatcgggcc
     2641 gctcacgagc cggtccccag gtaggcgctc tgcacggtcg cgttctccat gatccgctgc
     2701 ggcgtgtcca ccgcgatgat gctgccgaag tacatcacgg cgaccacgtc gaccaggccc
     2761 atcagcacct cgatgtgatg ctcgaccatg agcacggtgc atcctgtctc ggcctggagt
     2821 ccgcggatgc tgtcgacgag gcccgcgacg tcgccggagg cgacgccggc catgggctcg
     2881 tcgagcagca ccagacggga gcgggtggcc agcagcacgg cgatctcgag cttgcgcttg
     2941 tcgccgtggc tgatgtcccc cgcacgcgcg tccagccggt gccccagacc cacggagcgg
     3001 agcagctcct cggcgcgggc gctggcggca tccgagcgcc gcgggaagtg gaacagcgag
     3061 tagctgccgt tgtccgtggc ctggaccgcc atgcgcacgt tctcgcgcac gctcagctgc
     3121 gggaacagac tcgaggtctg gaacgtcctc cccagtcccg cccgggcgcg ctgcggcacg
     3181 ctcgtacggg tgatgtcggc gccgtccagc aggatgcgac ccgccgtggg cctgatcact
     3241 cccgagatgg tgttgaacag cgtcgtcttg ccggcgccgt tcgggccgat cactccgacg
     3301 agggcgccgg gctcgacggc gagatcgacg cccttgagga tcgtcgctcc cccgatctcg
     3361 agtccgagac cttcgacgcg gagtgacgcg ccggacgcgg gcggggctgg ggtggtcact
     3421 tcctacttct ccgcgggtgc gacgtcggtg gccggtaccg tgtcgacgag ttcgggcgtg
     3481 aacgtgcctc cggaggccac gagcttcgcc tggtacatgt cctggatcag tgcgtggtcc
     3541 ttcgctcgga ccgtgttctc gcccttcgga ccctggaagg tgaagccctc gagcgccttg
     3601 atcatggcgt tcacctcgcc cttgccctcc ttgacggcgt gcacgagcat gatggccgcg
     3661 ttgaacccgt cagggctgaa gaggtcgggc tcggcgccct tcttcttcag cgcctcgacc
     3721 atggcggtgt cgaccttgtt gtcggccgcg cccccgaagt agtggttcag gaagttgatc
     3781 ttctcggatg cctcgccgta ggcgccgaac gtggcgcggt cgccgagtcc ggtcacgacc
     3841 gggatcgcat ccaggacgcc ctgctggctc atcgcctgcc acatggcgcc ggaggtggcc
     3901 cccgcccacg cgacgaacac gagatcgggc ttggacgcga gcacctgctg cgcgaagggg
     3961 gtgaactcgg tcacatcctc cgcgacgagc accggctcga ctgtggcgcc cttggcaccg
     4021 aggatggcct ggaccgccgc gacgttgccc tgcccgaagg cgttgttctg cgcgaacacg
     4081 gagatcttct tgcccttgat gtccttgagg aacgttcctg cggtcgcgac gtcctgcgcg
     4141 gactgacgtc cggagcggaa ggtgtactcg ttgacgccgg tgatggcatc ggcggccgca
     4201 gggcccgaga tgaagagcac cttgttgtgc gcggcctgct cggccatcgc gagtgcgacg
     4261 ccggaggagg cgctgcccat caggatgttc acgccatcgc cgatcagctc cttggccgcc
     4321 gtgaccgccg tgtcggggtc gccggcgtcg tcgcggtgct cgatgacgat cttggttccg
     4381 cccacggccc ccttgccgtc ggtggcgtag tccaggcccg cggcgaaggc gtcgagatac
     4441 tgcttgccgt agccggcgag cgggccggtc tcgctggtga tcacgccgac gcgcacctcg
     4501 gcgggaccct tgtcgccgcc
//
LOCUS       DATMSX010000106         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124840,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000106 DATMSX010000000
VERSION     DATMSX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124840"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..765
                     /locus_tag="VN041_03335"
     CDS             94..765
                     /locus_tag="VN041_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28111.1"
                     /translation="MTIEVLIADDQAMVRAGFAALLDAHGGIHVTGQAADGLEAVALS
                     ARLDPDVILMDVRMPGLDGIEATRRILGPSYPAAKVPRILMLTTFDIDDYVYDALEAG
                     ASGFLLKDALPDELVHAVRVIADGDALLSPRVTRRMIEHFSTRRPRAPQSRTALNDLT
                     ERELEVLTLIGRGRSNHEIGTELFIAEQTVKTHVGKVFAKLGLRDRVHAVILAYDAGL
                     VEPAS"
     gene            854..2161
                     /locus_tag="VN041_03340"
     CDS             854..2161
                     /locus_tag="VN041_03340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWU28112.1"
                     /translation="MTSPRSIRLPEPTRDPAVDLVRAGCVIAVILLHSLMVGVTVTAQ
                     GPVFENAGDSGWWLTPVSWLLQVMPLFFVIGGFSGSIALRRSRERGGDGIRFTAERLH
                     RLLRPALVTVSVVGILLAVLERCGVPAELVATAGYRFGQPLWFLGVFLLCQALLPVML
                     RLHERHPLVTVSMLAGAAVLVDGVRAATGAGGVGFANLVFVWLVLQQVGFFLADGRID
                     ALGRHTRSVVLMASVLALVGSFVLGFHSPDLIANINPPTTALLLVGIAHTAAFSLLRT
                     RIAGFARRPRPAAFTAFVSRRTMTVYLWHMPVLLTMAGVLALWALTTGSSLPAPASPE
                     WWAGRPLWLAIVFTATAALSLPLARIEGKRVPLASPAPAVVTSAVLIALVAVLLLLVH
                     GTTTLTAVLAVAGWLLALRMVRPAAPRIAGATQEHTASALVTA"
     gene            complement(2191..2517)
                     /locus_tag="VN041_03345"
     CDS             complement(2191..2517)
                     /locus_tag="VN041_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HWU28113.1"
                     /translation="MIRVGDHVVLGALVDDRTRCAHYHGALDVVALRFHCCDAWYPCV
                     HCHDDVAGHDRTTWAASDGEIEAALCGACGTSMTIRAYRDAEACPACGAGFNPGCALH
                     HHLYFD"
     gene            complement(2514..3110)
                     /locus_tag="VN041_03350"
     CDS             complement(2514..3110)
                     /locus_tag="VN041_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HWU28114.1"
                     /translation="MISLYRPGTGILHRTPAGAKLAVLAASALVLSLLRPGIIGTLAV
                     LVLVSSLYVLGGLTLRALGEAWWRLRWLIVVLGGALWLFMSAADAVQNTGRVVALLLL
                     AELVTRTTRMGDLLEVFRALVRPLRRFGVDPEAVALTLSLTIAMIPVIAGFATLVRDA
                     QRARGVRLGPRAVLPLLVLTMKHADDVGDAMAARGIAA"
     gene            complement(3107..3745)
                     /locus_tag="VN041_03355"
     CDS             complement(3107..3745)
                     /locus_tag="VN041_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28115.1"
                     /translation="MGDAVLLHDVDLRLTAGRTAVIGANGSGKSTFARMLNGLRLPTA
                     GGLRVHGLDPMRDAKALRRHVGFVFTNPEAQLLMPTPAEDLALSLPNVPRSDRAARVA
                     RILAEYDLADRADVPVSGLSGGQRQLLAIASVLATDPGLVVADEPTTLLDLRNARRIG
                     DLLLGLDVPLVLVTHDLELAARCDEAVLFEDGTVRAVGEPAAVIDEYRRSCA"
BASE COUNT          538 a         1406 c         1262 g          547 t
ORIGIN      
        1 cggcggagaa ctgcgtgccg gcgtcgacgg caacggctgg ctcgtcagcg cccgcatccc
       61 ccgccacccc gccgacgccc cggaaggaac atcgtgacga tcgaggtcct catcgccgac
      121 gaccaggcca tggtgcgcgc cggcttcgcc gcgctcctgg acgcccacgg cggcatccac
      181 gtcaccggcc aggccgccga cggcctggag gccgtcgcac tgtcggcccg gctggatccg
      241 gacgtgatcc tgatggatgt ccggatgccg gggctcgacg gcatcgaggc gaccaggcgg
      301 atcctcggcc cttcgtaccc ggcggccaag gtgccgcgca tcctgatgct caccaccttc
      361 gacatcgatg actacgtgta cgacgccctc gaggcgggcg cgagcgggtt cctgctcaag
      421 gacgcactgc cggacgagct cgtccacgcc gtacgggtga tcgccgacgg cgatgcgctg
      481 ctctcgccac gggtgacgcg caggatgatc gagcacttct cgacgcgccg gccccgcgca
      541 ccgcagtcca ggacagcact gaacgatctc accgagcgcg agctcgaggt gctcaccctg
      601 atcgggcggg gccgttccaa ccacgagatc gggacggagc tgttcatcgc ggagcagacc
      661 gtcaagaccc atgtcggcaa ggtgttcgcc aagctcgggc tgcgcgaccg ggtgcacgcg
      721 gtgatcctcg cctacgacgc cggtctggtc gagcccgcct cctgatcacg gccgaggacc
      781 gcaccacggt atgagacgct tcgaccccgt ggggtgatga tgccgcaggc atcgcgtcca
      841 tagtgtccgt ggcatgacct cgccacgcag catccgcctc cccgagccca cccgcgaccc
      901 cgccgtcgat ctcgtccgag cagggtgcgt gattgccgtg atcctgctgc attctctgat
      961 ggtcggtgtg accgtcaccg cgcagggccc ggtgttcgag aacgccggcg acagcggctg
     1021 gtggctgacg ccggtcagtt ggctgctgca ggtgatgccg ctgttcttcg tgatcggcgg
     1081 cttctccggc tccatcgcgc tgcgacgcag tcgggagcgc gggggcgacg gcatccgctt
     1141 caccgccgag cgcctgcacc gcctgctgcg ccccgcattg gtcacggtgt ccgtcgtcgg
     1201 gatcctcctc gccgtgctcg aacggtgcgg tgtgcccgca gaactggtcg cgacggccgg
     1261 ttaccgcttc ggccagccgc tgtggttcct gggcgtcttc ctgctctgcc aggcactgct
     1321 gccggtgatg ctgcgcctgc acgagcgcca cccgctggtg acggtgtcca tgctcgccgg
     1381 cgcggccgtg ctggtcgatg gggtccgtgc agccaccggc gcaggcgggg tgggcttcgc
     1441 caacctcgtg ttcgtctggc tggtgctgca gcaggtcggc ttcttcctcg ccgatggccg
     1501 gatcgacgcc ctcgggcgcc acacgcggtc cgtcgtcctc atggcatcgg tcctggcgct
     1561 cgtggggtcg ttcgtgctgg gcttccactc ccccgacctc atcgcgaaca tcaacccgcc
     1621 gaccacggcg ctgctgctcg tcggcatcgc gcacactgcc gcgttctcgc tgctgcgcac
     1681 ccgcatcgcg ggtttcgccc gcaggccgcg cccggccgcg ttcaccgcat tcgtctcgcg
     1741 ccgcacgatg accgtgtacc tgtggcacat gcccgtgctg ctcacgatgg ccggggtcct
     1801 cgcgctctgg gccctgacca ccggctcgtc gctgccggcg ccggcgagtc cggaatggtg
     1861 ggcgggccgt ccgctctggc tggccatcgt cttcaccgcg accgccgcgc tcagccttcc
     1921 gctcgcgcgc atcgaaggga agcgggtccc cctcgcgagt ccagcccccg ccgtcgtcac
     1981 ctcagccgtc ctcatcgcgc tggtagccgt gctcctgctg ctcgtgcacg gaaccacgac
     2041 gctgaccgcc gttctcgccg tggccggatg gctactggcc ctgcggatgg tgcggccggc
     2101 ggcgccgcgg atcgccgggg cgacacagga gcacacggcc tcggcgctgg tgaccgcgtg
     2161 agcacccgcg ggatgccagg tcacggcagc tcagtcgaag tacagatggt ggtgcagcgc
     2221 acaccccggg ttgaaccccg ccccgcacgc cggacaggcc tcggcgtcgc ggtaggcgcg
     2281 gatggtcatg ctcgtgccgc acgccccgca cagcgccgct tcgatctcgc cgtcggacgc
     2341 cgcccacgtc gtgcggtcat gcccggccac gtcgtcatga cagtgcacgc acggatacca
     2401 ggcatcgcag cagtggaatc gcagcgcaac gacatcgagc gcaccgtggt agtgcgcgca
     2461 cctcgtccga tcgtcgacca gggcgccgag cacgacgtga tcgccgacgc ggatcacgcg
     2521 gcgatccccc gcgccgccat ggcgtctccg acgtcatcgg cgtgcttcat cgtgagcacg
     2581 agcagcggca gcacggcgcg cgggccgagc cgcacacctc tggcccgctg cgcgtcgcgc
     2641 accagggtgg cgaagcctgc gatcaccggg atcatcgcga tcgtcaggga cagggtcagc
     2701 gccaccgcct ccggatccac cccgaacctg cgcagcggcc gcacgagcgc acggaagacc
     2761 tcgagcaggt cgcccatccg cgtcgtgcgg gtcaccagct ccgccagcag caggagcgcg
     2821 acgacccggc ccgtgttctg cacggcatca gcggccgaca tgaacagcca cagcgccccg
     2881 cccagcacga cgatcaacca gcgcagccgc caccaggcct cccccagcgc gcgcagcgtc
     2941 aacccgccga gcacgtacag cgatgagacg aggaccagca ccgcgagtgt gccgatgatc
     3001 ccgggccgca gcagggacag caccaatgcc gaagccgcca gcacggcgag cttcgcaccg
     3061 gcgggcgtcc gatgcaggat gcccgtcccc ggccggtaca gcgagatcac gcgcagctcc
     3121 gccggtactc gtcgatcacc gctgcgggct ccccgaccgc ccgcacggtg ccgtcctcga
     3181 agagaaccgc ctcgtcgcac cgcgctgcca gctccagatc gtgcgtgacc agcacgaggg
     3241 gcacgtcgag ccccagcagc aggtcgccga tcctgcgggc gttgcgcagg tccagcagcg
     3301 tcgtgggctc atcggcgacg acgagtcccg gatccgtcgc gaggacggag gcgatggcca
     3361 gcaactgccg ctgccctccg gagagccccg ataccggcac atccgcgcgg tcggcgaggt
     3421 cgtactcggc gaggatccgc gcgacccgcg ccgctcgatc ggatcgcggc acgttgggaa
     3481 gcgagagcgc gaggtcctcc gcgggcgtcg gcatcaggag ctgcgcctcc ggattcgtga
     3541 agacgaagcc gacgtggcgt cgcagcgcct tcgcgtcgcg catcggatcc agaccgtgca
     3601 cccgcaagcc ccctgcggtc gggagcctca ggccgttcag catccgtgcg aaggtcgact
     3661 tgccggagcc gttcgcgccg atgacggccg tgcggcccgc ggtcagacgc aggtcgacgt
     3721 cgtgcagcag caccgcgtcg cccacggcga gac
//
LOCUS       DATMSX010000107         8554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20224,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000107 DATMSX010000000
VERSION     DATMSX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20224"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VN041_03360"
     CDS             complement(<1..421)
                     /locus_tag="VN041_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28116.1"
                     /translation="MPLFGSSFHALHDRSGLDIVFGGIARPRANHFAISHTLGGRTDR
                     LNGLVIRTGHGLGGLVMASARAATVRDYLSDGHITHAYDAAVAAERMRTVSAFPVIVK
                     GDVRGVIYGATRDAAMLTPEASAAMKRVADGFGYDLAV"
     gene            complement(490..2079)
                     /locus_tag="VN041_03365"
     CDS             complement(490..2079)
                     /locus_tag="VN041_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU28117.1"
                     /translation="MSNGASYASGTSTTPLLGQTIGGMLRETTARFGEREALVDSASG
                     RRWTFAQLLHDSERLASALRELGIRKGDRVGIWAPNCAEWVLTQYATALIGAILVNVN
                     PAYRTHEVKYVIEQSGMALIVSAVEYRTSKYAEMLTEIGYEQTLFIGTDRWDQLIDAA
                     PAFEDTDALAFDDPINIQYTSGTTGFPKGATLTHHNILNNGFFVGEGIAYTEQDRVAL
                     PVPLYHCFGMVMGILGAHSHGAAAVLPAPGFDAEITLQVVQDERITSLYGVPTMFIAE
                     LSHPRFTDFDLSSLRTGIMAGSPCPVEIMERTMNEMGMSEVAICYGMTETSPVSTMTK
                     MDDDVAARTQTVGQVMPHVEVKIIDEDGQVVPLGVPGELCTRGYSVMLGYWNEPSKTA
                     EAIDAGRWMHTGDMATMDENGRLQVVGRIKDMVIRGGENIYPREIEEFLYSHPDVQDV
                     QVIGVPDEQYGEELMAWIVLRPGASALTAEDVRMFATGKLAHFKIPKYVHLVDAFPIT
                     ITGKVRKVEMREMAVQLLAGR"
     gene            complement(2136..4220)
                     /locus_tag="VN041_03370"
     CDS             complement(2136..4220)
                     /locus_tag="VN041_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWU28118.1"
                     /translation="MAHDARENTDAPQLAKRILLGDPLTTEQVNDQLLPKRMALPIFA
                     SDALSSVAYGPQEMLMIMLIGGTAFLTLSPWVAAAVVLLLIVVVLSYRQIIKAYPSGG
                     GDFEVTQKNLGEVPSVIVAAALLVDYVLTVSVSIASGVDNIISAVPGLNPWRVELAVG
                     FVILIVIVNLRGVREASTVFAIPTYVFIGSVVVMIGTGLVRMLLGDAPVAASAAYTVQ
                     AESLSQAAMVLLILRAFSSGCSALTGVEAVSNGVPAFRVPKIRNAKTTLAMMGSIAAL
                     LFAGLTALALISGVKYAENACDLVGFDCSNPQPSLMAQVASATFGAGSIPFFIIQAAT
                     ACVLLLAANTAFNGFPLLGSVLASEGYAPKALNTRGDRLVFSNGMILLGLAAVVVLVV
                     FQANLTNLIQLYIIGVFVSFSLGQIGMVRHWRRVLRETHHRPLSEVATERRSAIVSLS
                     INSLGAVMTVSVLIIVTITKFTHGAWMVFFAIPVLALLMLGVKRYYRDVEKEIAIEDT
                     THFGSRGDVAIVLVNRMQKPVIKALDYAIAARHEKTIALHVALTPEDGQVLQRQWQEY
                     KIPVPLVIVDSPYRTFAQPVEQYIRKYREKHGPAVVTVYLPQYIVGHWWESVLHNRRS
                     RRMANQLMLVHGVSITLVPWLLDSSELIYGRRSRPLPGQERAGRPFALYGGDQRSGER
                     RVHRPAGPPDQG"
     gene            4283..5338
                     /locus_tag="VN041_03375"
     CDS             4283..5338
                     /locus_tag="VN041_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWU28119.1"
                     /translation="MSQQVMSGAIAIGIGVLVGIGLFVPFVAISYRRRGRLSPVRLLM
                     WLAALIYFWAIWTYTLLPLPEPSSIRCVGAITDPMSVVRDVQQALAAPGNPLTHPGIL
                     QLAFNVLLFVPLGFFLRVLGGRGVVTALLVGLGLSLFIETTQLTGVWGVYPCAYRFFD
                     VGDLMTNTTGALLGSVFGLVVPRAHRGLSRAADAGMPRPVTRGRRALAMICDGLGFFL
                     VSATLGVGMQLFLEYVVRDRAAVLDGTLSELVGTGGAGAFWLATILATGRSVGDLAVE
                     LRYDGARMPQPVARLLRWVGGVAGLSTLGLIGGIAVPLTSVAMFVACVMFFFTRRGRG
                     LPGILSGQHLADARSPR"
     gene            complement(5375..6313)
                     /locus_tag="VN041_03380"
     CDS             complement(5375..6313)
                     /locus_tag="VN041_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HWU28120.1"
                     /translation="MSHHPYRTFRTTVARIQKLTPSFTRVTLTGDDLAHFGTDGLDQR
                     IKLIIPFADGTITDVGQFDETQSMTEWYRRWRELPAEERNPIRTYTIRSPRPADREID
                     VDFVLHGTEGPASAWATGAQVGDPLFVVGPDSRSDEQGGGIEWKPGDATSVLIAGDET
                     AMPAICSIVESLPAHITGEVCIEVPSEADAVPLAAPEGVRVSWLGRGSTAHGVRLSAA
                     VHAWGRRRGASSASAHGAEPVRGLDEDDRPDASDASDEILWEVPEAASGGEYAWLAGE
                     AGTITALRRHLVRDLGIDRHSIAFMGYWRRGRAEAS"
     gene            6343..7206
                     /locus_tag="VN041_03385"
     CDS             6343..7206
                     /locus_tag="VN041_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28121.1"
                     /translation="MTNPKESSEARTDELHGESLALSYGRNRVVHEVSLHLRPGVVTA
                     LIGPNGSGKSTALRALARLHRVDEGIVRVVDDRGERDAAALSAREFARTVAMLSQSRP
                     HPSGLEVRDVVAYGRHPHRRRFAGMTDADRDAVAQALELTGLDHMSQRAVDELSGGEL
                     QRVWLATALAQDTGILLLDEPTNHLDLRYQVETLDLICDLADRGTAVGVVLHDLSHAA
                     SVADEVVLMHRGRVHASGLPHQVLTSENLTEVYGLPIDADHDPRTGRVRVAPRGRHHD
                     RARRGLAALSA"
     gene            7285..8316
                     /locus_tag="VN041_03390"
     CDS             7285..8316
                     /locus_tag="VN041_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU28122.1"
                     /translation="MNKPLAALATLGALTLALTACGTTTAAPAPTDAKTSTASGVGCA
                     DDSTSTSTKPVELTDALGRTVKLDKPAAKVAVLEWQQIEDVLSLCLTPVAVADADGYR
                     TWDTAEELPKDVKSVGTRQEPNLDALFATEPDLVIVEAGTRDDAIITQLEKYGVPVLS
                     TTGADAKDPIKQMLATFDLIAQATGRTERADVVKDEFEKKLAEGEKAVAGASLEGAPF
                     VFFDGWVDGGNVSLRPFGQGSLIGELGERLGLENAWTGEVDPAYGLGQTDIEGMTTVG
                     DAMLLHTGTADPDSESFIDAAAKNPAWASIPAVKDGRIHAFPAGIWTFGGPRSAEQIV
                     DAYVAVITK"
     gene            8349..>8554
                     /locus_tag="VN041_03395"
     CDS             8349..>8554
                     /locus_tag="VN041_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28123.1"
                     /translation="MTPRRGAVPAAGVLLALLVVLVVVSLWHLTQGTSGAVFADTDVL
                     FGSRVPRLAAGIAVGAALGVAGLL"
BASE COUNT         1258 a         3002 c         2943 g         1351 t
ORIGIN      
        1 ccacggccag gtcgtagccg aacccgtcgg cgacgcgctt catcgccgcg gacgcctcgg
       61 gagtgagcat cgcggcatcc cgcgtggccc cgtagatcac gccgcgcacg tcgcccttca
      121 cgatcaccgg gaaggcgctg acggtgcgca tccgctccgc cgccaccgcg gcgtcgtagg
      181 cgtgcgtgat gtgcccgtca ctgaggtagt cgcgcaccgt cgccgcacgg gcggatgcca
      241 tcaccagccc gcccaggccg tgaccggtgc ggatcaccag accgttcagc cggtcggtgc
      301 ggccgccgag cgtgtgcgag atcgcgaagt ggttggcgcg cgggcgcgcg atgccgccga
      361 agacgatgtc cagtccgctg cggtcgtgca gcgcgtggaa tgaggatccg aacaacggca
      421 tcccgcacct cctgccgggc ggtgccgcgg tgccgccgcc cgccatagaa cctacaccgc
      481 gctcggcgct cagcgcccgg cgagcagctg caccgccatc tcgcgcatct ccaccttgcg
      541 caccttgccg gtgatcgtga tggggaacgc gtcgaccagg tgcacgtact tcgggatctt
      601 gaagtgcgcc agcttcccgg tcgcgaacat ccgcacgtcc tcggcggtca gcgcggaagc
      661 gccgggccgc agcacgatcc acgccatcag ctcctcgccg tactgctcgt ccggcacccc
      721 gatcacctgc acgtcctgca cgtccgggtg cgagtacagg aattcctcga tctcgcgcgg
      781 gtagatgttc tcgccgccgc ggatcaccat gtccttgatg cggccgacca cctgcagccg
      841 tccgttctcg tccatggtcg ccatgtcgcc ggtgtgcatc cagcgccctg cgtcgatggc
      901 ctcggcggtc ttcgagggct cgttccagta accgagcatc accgagtagc cgcgcgtgca
      961 cagctcgccc ggaacgccca gaggcaccac ctggccgtcc tcatcgatga tcttgacctc
     1021 gacgtgcggc atcacctgtc cgaccgtctg cgtgcgggcg gcgacgtcgt cgtccatctt
     1081 ggtcatggtc gacacgggcg aggtctcggt catcccgtag cagatggcca cctcgctcat
     1141 ccccatctcg ttcatggtgc gctccatgat ctccaccggg cacggcgagc cggccatgat
     1201 ccccgtgcgc agcgaagaca ggtcgaagtc cgtgaagcgc ggatgcgaca gctcggcgat
     1261 gaacatggtc ggcactccgt agagcgaggt gatgcgctca tcctgcacga cctgcagcgt
     1321 gatctcggcg tcgaagcccg gcgccggcag cacggcggcg gcgccgtgcg agtgcgcgcc
     1381 gaggatgccc atcaccatgc cgaagcagtg gtacagcggc actggcagcg ccacccgatc
     1441 ctgctcggtg tacgcgatgc cctcgcctac gaagaacccg ttgttgagga tgttgtggtg
     1501 ggtcagcgtc gcgcccttcg ggaaccccgt ggtgcccgag gtgtactgga tgttgatcgg
     1561 gtcgtcgaag gccagggcat ccgtgtcctc gaacgcgggc gccgcgtcga tcagctgatc
     1621 ccagcggtcg gtgccgatga acagcgtctg ctcgtagccg atctcggtga gcatctcggc
     1681 gtacttcgac gtgcggtatt cgaccgcgct gacgatcagc gccatcccgg actgctcgat
     1741 cacgtacttc acctcatgtg tgcgatacgc ggggttgacg ttcaccagga tggcgccgat
     1801 gagcgcggtc gcgtactgcg tcagcaccca ctcggcgcag ttcggcgccc agatgccgac
     1861 ccggtcgccc ttgcggatgc cgagctcgcg cagcgccgag gccaggcgct cgctgtcgtg
     1921 cagaagctgc gcgaaggtcc agcggcggcc ggatgccgag tccaccaggg cttcgcgctc
     1981 gccgaagcgg gcagtcgtct cacgcagcat cccgccgatc gtctggccga gaagtggggt
     2041 cgtgctggtt ccggatgcat aggaagcgcc attgctcatc gcatcaggat gctcccgagc
     2101 cggctacccg tccacccccg tacgggggtg atcgctcagc cctggtccgg gggaccggca
     2161 ggccggtgca cgcggcgctc gccgctccgc tggtcgccgc cgtacagggc gaacggccgg
     2221 ccggcgcgct cctggcccgg aagtgggcgc gaacggcgtc cgtagatgag ctcggacgag
     2281 tcgagcagcc acggcacgag cgtgatcgag actccgtgca cgagcatcag ctggttggcc
     2341 atccggcggg agcggcggtt gtgcagcacc gactcccacc agtggcccac gatgtactgc
     2401 ggcaggtaga ccgtgaccac cgcgggaccg tgcttctcgc ggtacttgcg gatgtactgc
     2461 tcgaccggct gtgcgaaggt gcggtacggg gagtccacga tcaccagcgg cacggggatc
     2521 ttgtactcct gccactgccg ctgcagcacc tggccgtcct cgggcgtgag ggcgacgtgc
     2581 agcgcgatcg tcttctcgtg acgtgcggcg atcgcatagt ccagggcctt gatgaccggc
     2641 ttctgcatcc ggttcaccag cacgatcgcg acgtcgccgc gcgacccgaa gtgggtcgtg
     2701 tcctcgatcg cgatctcctt ctcgacgtcg cggtagtacc gcttgacgcc cagcatcagc
     2761 agagcgagca cagggatggc gaagaacacc atccaggcgc cgtgggtgaa cttcgtgatg
     2821 gtcacgatga tgagcacgct cacggtcatc acggcaccga gggagttgat ggacaggctc
     2881 acgatggccg agcgccgctc cgtggccact tcgctcagcg gtcggtgatg cgtctcgcgc
     2941 agtacgcgcc tccagtgccg caccatgccg atctggccga gcgagaacga cacgaacaca
     3001 ccgatgatgt acagctggat gaggttggtg aggttcgcct ggaacaccac cagcacgacg
     3061 accgcggcga ggcccagcag gatcatgccg ttggagaaca ccagccggtc gccgcgggtg
     3121 ttgagcgcct tcggggcgta gccctcgctg gccagcaccg agcccagcag cgggaagccg
     3181 ttgaacgccg tgttggcggc cagcagcagc acgcacgcgg tggcggcctg gatgatgaag
     3241 aacgggatgc tgccggcgcc gaacgtcgcc gatgcgacct gcgccatcag gctcggctgc
     3301 gggttggagc agtcgaagcc gaccaggtcg caggcgttct cggcgtactt gacgcccgag
     3361 atcagcgcca gtgcggtgag gccggcgaac agcagcgcgg cgatcgagcc catcatcgcc
     3421 agggtggtct tggcgttccg tatcttcggc acgcggaacg ccggcacgcc gttcgacacg
     3481 gcctccacgc ccgtcagggc ggagcatccg ctggagaacg cgcgcaggat cagcagcacc
     3541 atcgccgcct ggctgaggct ctcggcctgc accgtgtacg ccgcgctcgc cgccacgggg
     3601 gcgtcgccga gcagcatccg caccagtccg gtgccgatca tcacgaccac cgagccgatg
     3661 aacacgtacg tcgggatcgc gaagacggtg gatgcctcgc gcacgccgcg caggttgacg
     3721 atgacgatca ggatcacgaa gccgaccgcc agctccacac gccatgggtt caggcctggt
     3781 accgccgaga tgatgttgtc gacgccggac gcgatcgaca cggagacggt gaggacgtag
     3841 tcgaccagca gtgcggccgc gacgatcacc gacggcacct cgccgaggtt cttctgcgtc
     3901 acctcgaagt cgccgccgcc ggaggggtac gccttgatga tctggcggta gctgagcacg
     3961 accacgatca gcagcagcac gacggccgcc gcgacccacg ggctgagcgt caggaacgcc
     4021 gtcccgccga tcagcatgat catgagcatc tcctgcggcc cgtaggccac cgagctcagc
     4081 gcgtcggacg cgaagatcgg cagggccatg cgcttgggga gcagctggtc gttgacctgc
     4141 tcggtcgtca gtgggtcgcc gagaaggatg cgtttggcga gttgcggggc atccgtgttc
     4201 tcgcgtgcgt cgtgtgccac gacgggcgag actacgcctg cgcgagcggc cgtgcaacac
     4261 ggcgcccgta gactttattc cgatgagcca gcaggtgatg tccggagcga tcgcgatcgg
     4321 catcggcgta ctcgtcggca tcggcctctt cgtgccgttc gtggcgatca gttaccgccg
     4381 ccgcgggcgg ctcagcccgg tacggctgct gatgtggctc gcggcgctca tctacttctg
     4441 ggcgatctgg acgtacacgc tgctgccgct tccggagccc tcgtcgatcc gctgcgtcgg
     4501 ggcgatcacc gaccccatgt cggtggtgcg ggacgtgcag caggcgctcg ccgcgccggg
     4561 caacccgctc acgcatccgg gcatcctgca gctcgcgttc aacgtgctgc tgttcgtgcc
     4621 gctgggcttc ttcctgcgcg tgctgggcgg acgcggtgtc gtcacggccc tgctcgtggg
     4681 cctgggcctg tcgctgttca tcgagaccac gcagctgacc ggggtctggg gcgtctatcc
     4741 ctgcgcgtac cggttcttcg acgtcggcga cctgatgacc aacacgacgg gtgccctgct
     4801 cggctcggtg ttcgggctgg tggtgcctcg ggcgcaccgt ggtctgtccc gtgcggcgga
     4861 cgccgggatg ccgcgccccg tcacccgcgg gcgccgcgcc ctggcgatga tctgcgacgg
     4921 gctcggcttc ttcctggtct cggcgacgct gggcgtcggg atgcagctgt tcctggagta
     4981 cgtggtgcgc gatcgtgccg ccgtgctgga tgggaccctg tcggagctgg tcggcacggg
     5041 cggtgcgggg gcgttctggc tcgcgacgat cctggcgacc ggccgatcgg tcggagacct
     5101 cgcggtggag ctgcgctacg acggagcgcg catgcctcag ccggtcgcgc gtctgctgcg
     5161 ctgggtcggc ggtgtggccg gtctgtcgac gctcgggctg atcggcggga tcgccgtgcc
     5221 gctgacgagc gtcgcgatgt tcgtcgcgtg cgtgatgttc ttcttcaccc gtcggggccg
     5281 cggactgccc ggcatcctct ccggccagca cctggccgac gcccgctccc cgcgctgacg
     5341 tctcgcgggc cttcgggttc ggtattcggg cgggtcagga tgcctcggcg cggccgcggc
     5401 gccagtagcc catgaacgcg atcgagtgac ggtcgatgcc gagatcgcgc accaggtggc
     5461 gccgcagagc ggtgatggtt cccgcctcgc cggccagcca cgcgtactcg ccgccggacg
     5521 ccgcctcggg aacctcccag aggatctcgt cggacgcgtc ggacgcgtcg ggcctgtcgt
     5581 cctcgtcgag tccgcggacc ggctctgcgc cgtgagcgga tgccgatgac gcgccgcgcc
     5641 tgcgccccca cgcgtgcacg gctgcgctca gccgcacacc gtgcgcggtc gatccgcggc
     5701 ccagccagct cacccgaact ccctcgggag cggccagggg gacggcatcc gcttcggacg
     5761 gcacctcgat gcacacctcg ccggtgatgt gcgcgggcag tgattcgacg atggagcaga
     5821 tcgcaggcat ggcggtctcg tcgcccgcga tcagcacgga ggtcgcgtct cccggcttcc
     5881 attcgattcc cccgccctgc tcgtccgagc gggagtccgg tccgacgacg aacagcggat
     5941 cgccgacctg cgcaccggtc gcccaggcgg atgccgggcc ctcggtgccg tgcagcacga
     6001 agtcgacatc gatctcgcgg tcagcgggac gcggggagcg gatcgtgtag gtgcggatcg
     6061 gattgcgctc ctcagcgggg agttcacgcc agcgccggta ccactcggtc atgctctggg
     6121 tctcgtcgaa ctgaccgaca tcggtgatcg tgccgtccgc gaaggggatg atcagcttga
     6181 tgcgctgatc caggccgtcg gtgccgaaat gcgccaggtc gtcgccggtc agggtgactc
     6241 gcgtgaagct cggcgtgagc ttctggatgc gggcgacggt ggtgcggaag gttcggtagg
     6301 ggtggtggct caccacttca gcataggtta ggctctccta acgtgaccaa tccgaaggaa
     6361 tcatccgagg cccgcacgga cgagctgcac ggcgaatcgc tcgccctcag ctacggccgc
     6421 aaccgggtgg tgcacgaggt gtcgctgcac ctgcgccccg gtgtcgtcac cgctctgatc
     6481 ggccccaacg gcagtggcaa gtccacggcg ctgcgcgccc tcgcccggct gcacagggtc
     6541 gacgagggga tcgtccgcgt ggtcgacgac cggggggagc gcgacgctgc ggcgctctcg
     6601 gccagggagt tcgcccgcac ggtcgcgatg ctctcccagt cccgcccgca cccctccggg
     6661 ctcgaggtgc gcgacgtcgt cgcctacggt cggcatccgc atcgccggag gttcgcgggg
     6721 atgaccgacg ccgaccggga cgctgtcgcc caggcgctcg agctcaccgg cctcgaccac
     6781 atgtcgcagc gcgccgtcga cgagctctcc ggcggggagc tgcagcgcgt gtggctcgcc
     6841 actgccctcg cgcaggacac cggcatcctg ctgctggacg agccgaccaa ccacctcgac
     6901 ctgcgctatc aggtcgagac cctcgacctg atctgcgacc tggccgaccg cggcaccgcc
     6961 gtgggcgtgg tgctgcacga cctgagccat gcggcatccg tcgccgacga ggtcgtgctc
     7021 atgcaccgcg gccgcgtgca cgcctcggga ctcccgcacc aggtgctcac gtccgagaac
     7081 ctcaccgagg tgtacggact gcccatcgac gccgaccacg acccgcgcac cggacgggtg
     7141 cgggtcgctc cgcgcggcag gcatcacgac cgtgcccgcc gcggcctcgc cgcgctctcg
     7201 gcctgatctc ccgcagcggc atcgccgcgc acttccctgt acccgattcc ccgaccccga
     7261 ccccgacatc ccggagccat cctgatgaac aagcccctcg ccgccctcgc cacgctcggc
     7321 gctctgaccc tcgcgctcac cgcctgcggc accaccacgg cggcccccgc gcccaccgac
     7381 gcgaagacct ccacggcgag tggcgtcggc tgcgccgacg acagcacctc cacttccacc
     7441 aagccggtcg agctgaccga cgcactgggc cgcaccgtca agctggacaa gcctgccgcg
     7501 aaggtcgccg tgctcgaatg gcagcagatc gaggacgtgc tctcgctgtg cctcacgccg
     7561 gtcgcggtgg cggatgccga cggctaccgc acctgggaca ccgccgagga gctgccgaag
     7621 gacgtcaaga gcgtcggcac ccgtcaggag cccaacctcg acgcgctgtt cgccaccgag
     7681 cccgacctcg tgatcgtcga ggcgggcacc cgcgacgacg cgatcatcac gcagctggag
     7741 aagtacggcg tcccggtgct cagcaccacc ggcgccgacg cgaaggaccc gatcaagcag
     7801 atgctcgcga ccttcgacct gatcgcgcag gcgaccggcc gcaccgagcg cgccgacgtc
     7861 gtgaaggacg agttcgagaa gaagctcgcc gagggcgaga aggccgtcgc gggtgcctct
     7921 ctggaaggcg cgcccttcgt gttcttcgac ggctgggtcg acggtggcaa cgtgtccctg
     7981 cgtccgttcg gacagggctc gctgatcggc gagctcggtg agaggctcgg gctcgagaac
     8041 gcctggaccg gcgaggtcga tccggcctac ggtctgggcc agaccgacat cgagggcatg
     8101 accacggtcg gcgacgccat gctgctgcac accgggacgg ccgatcccga ctcggagagc
     8161 ttcatcgacg cggcggccaa gaaccccgcc tgggcctcga tccccgccgt gaaggacggg
     8221 cgcatccacg cgttccccgc gggcatctgg accttcggcg gaccccgctc cgccgagcag
     8281 atcgtcgacg cgtacgtcgc cgtcatcacc aagtgaccga ggcgcgtctg gacacgcccg
     8341 ccgggggcgt gacgccccgg cggggcgccg tgcccgcggc gggcgtgctc ctcgcgctgc
     8401 tcgtcgtcct ggtcgtggtg tcgctgtggc acctcacgca gggcaccagc ggagccgtgt
     8461 tcgcggacac cgatgtgctg ttcggctcgc gggtgccgcg gctggcggcg gggatcgcgg
     8521 tgggggccgc cctcggcgtc gccgggctgc tcat
//
LOCUS       DATMSX010000108         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1659,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000108 DATMSX010000000
VERSION     DATMSX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VN041_03400"
     CDS             complement(<1..457)
                     /locus_tag="VN041_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28124.1"
                     /translation="MPPANANLLRNAMSDDGELTEIAYAKVNLALHVRARRDDGYHAL
                     ESLFVFARDGDVLTTKAAESGAITLKIEGSFADGLDSGGGNLVMKAAQALREYLGESR
                     GAALTLVKNLPVASGIGGGSADAAATLRLLGRLWNAHLPTPDLERIALSL"
     gene            502..1092
                     /locus_tag="VN041_03405"
                     /pseudo
     CDS             502..1092
                     /locus_tag="VN041_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            complement(1148..1498)
                     /locus_tag="VN041_03410"
     CDS             complement(1148..1498)
                     /locus_tag="VN041_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWU28125.1"
                     /translation="MSRISRDALVSRRADAIARAASAASALADLVHDRLGSNDDDEHD
                     PEGVPLSSEWSRLTALSEAADREVRQFDEALQRWDAGTYGICASCGRPIPEGRLEVRP
                     SAELCVPCAEKAGA"
     gene            1537..>2235
                     /locus_tag="VN041_03415"
     CDS             1537..>2235
                     /locus_tag="VN041_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28126.1"
                     /translation="MARRGAELPPTARMAWSRSIADAVIAAASAGGIGVALSCAIMII
                     AALRGASDTLQPIGLGILAVSAVAAALFSALLGPALAAVNLARMRMYLGAVPGSASLR
                     NAVEFRGQASSSPLFPLQVTLVCVAGAGALAAIVSGILWAQEPDAVYRDATLCCIGLV
                     VLTVLGVLICRGMTRNCLAPVLSDLPAGSVGQRDAEETILSDTRENRRLLAARARRTS
                     TPLDNARSVAGWCLA"
BASE COUNT          319 a          815 c          742 g          359 t
ORIGIN      
        1 ccagtgacaa ggcgatccgt tccaggtccg gcgtcgggag gtgcgcattc cacaagcgcc
       61 ccagcagccg caacgtcgcc gctgcgtccg ccgatccgcc gccgatgccg gacgccaccg
      121 gcaggttctt gaccagcgtt agcgccgcgc ctcggctctc acccagatat tcgcgcagcg
      181 cctgagccgc cttcatgacc agattaccgc cgccgctatc cagcccatcg gcgaaagagc
      241 cctctatttt caaggtgatc gcgcccgatt ccgccgcctt ggtggtcaga acatccccgt
      301 cacgcgcgaa tacgaacagg ctttccagcg cgtgatagcc atcgtcccgc cgcgcccgga
      361 catgaagagc cagattgacc ttggcgtaag caatttcggt caattctccg tcatcggaca
      421 tggcattgcg caacaggttc gcgttagcgg gcggcatatt ccggtttcca tccttccctt
      481 gcctttgacg ccagccgggc gaccgcggag gagggcgtcg cctaccggcg cgacatcctc
      541 taccgcctga tcgagatgtc ggcggaggac ggcatggtga tgcagctgca tcccggcatc
      601 catcgcaacc accacgtgcc cacgctgcgg cgcttcggag ccgacaccgg gcacgatctg
      661 ccggtgccga tgacctacac cgagccgctg cgacgcgcgc tgaacgactt cggcacgggt
      721 acgcagctgc gcctggtgct gttcacggtg gacgagacgt cgttctcacg ggaactggcg
      781 ccgatggcag gcttctaccc gagcgtgtac gtgggcgctc cgtggtggtt cctcgacacc
      841 ccggatgcca tggagcgcta ccgccgcgcg atcaccgaca gcgcaggctt cgcgaagacc
      901 tcgggcttca tcgacgacac ccgcgccttc tgctccatcc ccgcccggca cgacatggcc
      961 cggcgggtgg atgccggcta tctggcgtcg ctggtggtca cgcaccggct cagcgaggag
     1021 gatgcggccg agatcgccga cgacctcgtg cgccgcgtgc cctacgagac gttccgcatc
     1081 ccgcgggagt gacgccggcg gcgggtcggc ccccgacctg gggcgccccg atgccgagcg
     1141 ccaggtatca ggccccggcc ttctccgcgc acgggacgca cagctccgcc gacggcctga
     1201 cctccaggcg tccctccggg atcggcctgc cgcacgacgc gcagatgccg tacgtcccgg
     1261 catcccagcg ctgcagcgcc tcgtcgaact gccgcacctc ccgatcggcg gcctccgaga
     1321 gcgccgtcag ccgcgaccat tcggatgaca ggggaacacc ctccgggtcg tgctcgtcgt
     1381 cgtcgttcga gcccagccgg tcgtgcacga ggtcggccag ggcggatgcc gcggaggcgg
     1441 cgcgggcgat cgcatccgct ctccgtgaga cgagcgcatc gcgactgatc cggctcatcg
     1501 actcacagta gcgtgagagg tgggagggga ggcccgatgg cacgacgagg cgcggaactg
     1561 ccgccgacgg cgcgcatggc ctggtcgcgc agcatcgcgg atgccgtgat cgccgcggcc
     1621 tctgcgggag ggatcggcgt cgcgctcagc tgcgcgatca tgatcatcgc cgcgctgcgc
     1681 ggggcatccg acactctgca gccgatcggt ctcggcatcc tcgccgtcag cgcggtcgcc
     1741 gccgcgctct tctcggcgct gctcgggcct gcgctcgcgg ccgtgaacct ggcgcggatg
     1801 cgcatgtacc tcggagccgt gcccggttcg gcctcgctgc gcaacgccgt cgagttccgt
     1861 gggcaggcga gctcttcgcc gctgttcccg ctgcaggtca cgctggtctg cgtggcaggg
     1921 gccggagcgc tggcggcgat cgtctccggg atcctctggg cgcaggagcc ggatgccgtc
     1981 taccgcgacg cgacgctctg ctgcataggg ctcgtcgtgc ttaccgtgct cggtgttctg
     2041 atctgccgcg gcatgacacg gaactgcctc gcgcccgtgc tctccgacct ccctgcgggg
     2101 tcggtcgggc agcgcgatgc ggaggagacg atcctgtcgg acacccgcga gaaccgtcgc
     2161 ctgctcgccg cccgcgctcg ccgaacgagc accccactcg acaacgcgcg gtccgtggcc
     2221 ggctggtgcc tggcc
//
LOCUS       DATMSX010000109         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_86884,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000109 DATMSX010000000
VERSION     DATMSX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86884"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..597)
                     /locus_tag="VN041_03420"
     CDS             complement(64..597)
                     /locus_tag="VN041_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU28127.1"
                     /translation="METTQNQTNSGLTGIHTTDGRPNRSTSLTPFLAIPDAAAAIAFY
                     RDVFGARVIDVTEFDGVVAHADLDFGLGGLQLGEPSPDYHLVAPPQGEDDCYSMGIYV
                     PDVDAVVARAVEAGAVVREAPSDFVSGDRFASIRDPFGVRWSVMSRVEDLAEEESARR
                     VAEWAASFSAAQEDTAG"
     gene            679..1455
                     /locus_tag="VN041_03425"
     CDS             679..1455
                     /locus_tag="VN041_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28128.1"
                     /translation="MIDARRGVLYPDRMPRFHRLPPTPDAQHLAAWFWIPEWDLDPGE
                     TSRQEIVAYPALNLVVEPSGVQLVGATTTATHRDLTGRGWAVGALLRPAAVAALTEKP
                     SELRDSWTMLAAPDLLEPIAAVMDGDDDDRLPHAVAIMSGWLAERAGEPTDAGLQANA
                     MSELLMTDPGIRSAEQAATRLAVSLRTLQRMTHRHVGLPPLAMIRRRRLQEAAQRLRD
                     DPGIDLAALAAELGYADHAHLTGDFRTVLGLAPSGYRQEF"
     gene            complement(1462..>1824)
                     /locus_tag="VN041_03430"
     CDS             complement(1462..>1824)
                     /locus_tag="VN041_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWU28129.1"
                     /translation="AREADLPRWLAAIDPRWQVPRRAEITIGLIVIAVVLIADLRGAI
                     GFSSFGVLLYYLLANASAWRQSADVRRYPRALQAIGMLGCLALVVTLPVLGVIVGAEV
                     VLAGVAYRLLRLRFASRS"
BASE COUNT          275 a          651 c          651 g          247 t
ORIGIN      
        1 gtcgtcgtcg cgctgacggc gcccgtgacc ggctgactcc cgcccgggcg ccgcatgtgc
       61 tgatcagccg gcggtgtcct cctgggcagc cgagaacgat gccgcccact cggcgacgcg
      121 gcgggcgctc tcctcctcgg cgaggtcctc gacgcggctc atcaccgacc agcgcacccc
      181 gaagggatcg cggatgctgg cgaaccggtc gcccgacacg aagtccgacg gcgcctcgcg
      241 caccacggcc ccggcttcga cggcgcgggc gacaacggca tcgacgtcgg gcacgtagat
      301 gcccatcgag tagcagtcgt cctcgccctg cggcggcgcg acgaggtgat agtcgggcga
      361 cggctcgccg agctgcaggc cgccgaggcc gaagtccagg tcggcgtggg ccacgacgcc
      421 gtcgaactcg gtgacgtcga tcactcgtgc gccgaacaca tcgcggtaga acgcgatggc
      481 cgccgccgcg tcggggatcg cgaggaacgg tgtcagcgag gtggagcggt tcgggcggcc
      541 gtcggtggtg tggatgccgg tgaggccgct gttcgtctgg ttctgcgtgg tttccatgat
      601 ccgacggtac gagcgacgcc tcaccccggg cttgaagatt cgcgacagat gtccgagacc
      661 gcgcctatcg tcgttggaat gatcgatgcg cgacgcggag tcctgtatcc cgaccggatg
      721 ccgcggttcc accggctgcc gccgacgccg gacgcgcagc acctggccgc gtggttctgg
      781 atccccgagt gggatctcga tccgggggag acgtcgcggc aggagatcgt cgcctatccc
      841 gccctgaacc tggtggtgga gccatccggc gtgcaactcg tcggagcgac gaccacggcg
      901 acccatcgcg acctgaccgg ccgagggtgg gccgtcggtg cgctgctccg tcccgccgct
      961 gtcgcggccc tgaccgagaa gccgtccgag ttgcgggact cgtggacgat gctcgccgct
     1021 cccgacctgc tcgaacccat tgcagccgtc atggacggcg atgacgatga ccggctgccc
     1081 cacgcggtcg cgatcatgtc gggatggctg gccgagcgcg cgggagagcc gacggatgcc
     1141 gggctgcagg ccaacgcgat gtccgagctg ctcatgaccg accccggcat ccgcagcgcc
     1201 gagcaggccg ccacgcgact cgcggtctcg ctgcggacgc tgcagcgcat gacgcaccgc
     1261 cacgtcgggc ttccgcccct cgccatgatc cggcgtcgac ggctgcagga ggcggcgcag
     1321 cggctgcggg acgatcctgg gatcgacctc gccgctctcg cggccgagtt gggctacgcc
     1381 gatcacgctc acctcaccgg cgacttccgc accgtgctcg gcctggcgcc gagcggctac
     1441 cggcaggagt tctgaccccg gtcaggaccg agacgcgaac cgcagtcgca gcagacggta
     1501 cgccacgccg gccaggacga cctccgcgcc gacgatgacc ccgagcacgg gaagcgtgac
     1561 cacgagcgcc aggcatccga gcatcccgat cgcctgcagc gctctcggat agcgccgcac
     1621 gtcggcggac tgccgccagg ccgaggcgtt cgccagcagg tagtacagca ggacgccgaa
     1681 ggacgagaag ccgatcgcgc cgcgcaggtc ggcgatcagc accacggcga tcacgatcag
     1741 tccgatcgtg atctcggccc ggcggggcac ctgccagcgc gggtcgatgg cggcgagcca
     1801 gcggggcagg tccgcctcgc gggc
//
LOCUS       DATMSX010000110         9383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_162463,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000110 DATMSX010000000
VERSION     DATMSX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162463"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VN041_03435"
     CDS             <1..150
                     /locus_tag="VN041_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HWU28130.1"
                     /translation="AASALGMSRVAARRYLEHLADDGVAVREARYGTRGRPETQYRWN
                     LRPPR"
     gene            complement(163..807)
                     /locus_tag="VN041_03440"
     CDS             complement(163..807)
                     /locus_tag="VN041_03440"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HWU28131.1"
                     /translation="MSERRIAVVSSGLSNPSSTRMLADRLAAETARALRERDIDVQVD
                     VIELRDLAHDITNNLLTGFAPAPLEEAINRVVSADAVIAVTPIFSTSYAGLFKSFIDV
                     LDPDALTGKPVLIGANAGTARHSLAIDYAIRPLFTYLHAEPVSTGVFAASSDWGANAD
                     DVAPLSARVERGARELAEAIARKEPVTDADPFDPDTYLGKGKSFGHLLGGLAGE"
     gene            complement(809..2008)
                     /locus_tag="VN041_03445"
     CDS             complement(809..2008)
                     /locus_tag="VN041_03445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU28132.1"
                     /translation="MQFGIMSVSDITQDPTTGITPSERERIQAAVTIAMHAEEVGLDV
                     FAIGEHHNPPFFSSSPTTLLAYIAAQTERLIVSTSTTLITTNDPVRIAEEYAMLQHLS
                     GGRMDLMMGRGNTGPVYPWFGQDIRQGLPLAIENYALLNELWTKDVVDWEGKFRTPLQ
                     GFTATPRPLDGVKPFVWHGSIRTPEIAEQAAYYGDGFFANNIFWPKEHYQRLIELYRQ
                     RWSHYGHGAPETAIVGLGGQAFIAKNSQDAVNRFRPYFDNAPVYGHGPSMEDFTEMTP
                     LTVGSPQQVIDRYAAMRDTFGDYQRQLFLMDHAGLPLKTVLEQIDLLGEEVVPVLRKE
                     LAKDRPATVPDAPTHAARVKAVYGDGPAREARPAANRGDNLTAGSPYQDAPAPAGAAF
                     GLSRKEA"
     gene            2169..3296
                     /locus_tag="VN041_03450"
     CDS             2169..3296
                     /locus_tag="VN041_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWU28133.1"
                     /translation="MSSADQHPPTTTPTGTNRGRRRVPFWDNARFACVVLVVIGHGTQ
                     RLIYDSDISYAFYLWIYAFHMPAFAIISGYFSKSASPTKRQMARVITDILVPYVIFEG
                     LWTLTKWLVEGRADPNLTQPSWTLWFLLALGIFRLVLPYLALLRWPLAWALAISVGVG
                     YLSNVDSTFSLSRTLGLMFFFTLGWWLRERDVVRRFSLIDYRPWWLRICAVAVLAVWA
                     FVCWRWLAFWDDVDLRFWFFYDDSYSGLSADQWWAGGIRLGLMALAILLSACFFVLLP
                     RRAQWWTTFGQYTMYVYLLHSFVLYPFRESGLLRGLDPTWIWLPLITVLAVAIAFLLA
                     SKPVRRVFRPLIEPRPKWMFTDAALSSGSDHRSDPTGSRRG"
     gene            complement(3300..4565)
                     /gene="efeB"
                     /locus_tag="VN041_03455"
     CDS             complement(3300..4565)
                     /gene="efeB"
                     /locus_tag="VN041_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HWU28134.1"
                     /translation="MTEDVEAGRKRPGLSRRGLLGLAAGGGAAALALAGGGGYALAAA
                     QRRDETASGVHAFFGTHQAGITTPVQEHLHFAAFDMMPRSDRADLIELLQDWSYAASR
                     MTQGLEVSATGAVGGPSEAPPDDTGEALGLPASSLTVTFGFGPTLFENEDGDRYGIRD
                     RRPAKLQRLPAFLGEDLDPTLSDGDLCVQVCADDPQVAVHAIRNLSRIAFGRAKLRWS
                     QLGFGKTSRTTAEQQTPRNLFGFKDGTANILADDAKALDDHVWVADGDDPAWLAGGSY
                     LVARKIAMLIETWDRVRLGEQERIFGRDKGEGAPLSGGTEGTAPKLPGLDAHSHVRLA
                     HPDSNGGTRILRRGFNYVGGNNSLGRLDAGLFFLAYQRDPEQFIALQRKLSTDLLNEY
                     IRHVGSGIWAVPAGARSGSYVGEALFRDA"
     gene            complement(4573..5805)
                     /gene="efeO"
                     /locus_tag="VN041_03460"
     CDS             complement(4573..5805)
                     /gene="efeO"
                     /locus_tag="VN041_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HWU28135.1"
                     /translation="MTLTARTLGAAAALAAAAIALTGCVAKATADPAASFTVKSTADA
                     CTVSADTAKSGTLTFDISNSGDQVTEFYLLASDGLRIVGEVENIAPSASRTMTVVAQP
                     GDYFTVCKPGMVGDGVGKSAFTVSGDKVAIDGPDAEQKQKAVDLYAAFVKDQVGQLVP
                     AVQELVNAYQSGDDAKARELFPNTRAYYERIEPVAEALGDLDPRIDFREVDAVADGLD
                     WTGFHRLEKDLWQPAQDALNSDGETSAWKDWAPSTPEQRGEFGDLLISDVQELYDYVH
                     SKDFTTALNDQGIAGISNGAVALLDEVATGKISGEEDWWSHTDLYDFAANVEGSKMAF
                     SLVSDLVASKGADGKALVEEISTGYADLEKALAAQGSLDAGFVPFTQLNDADKRELID
                     LINALAEPLSKLTSTALA"
     gene            complement(5802..6740)
                     /gene="efeU"
                     /locus_tag="VN041_03465"
     CDS             complement(5802..6740)
                     /gene="efeU"
                     /locus_tag="VN041_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HWU28136.1"
                     /translation="MLPALLIGLREGLEAALVVGILVAYVRRLGRADVLGRMWIGIGL
                     AIALALGIGAVLTFGAYALTSEAEEIIGGTLSLVAVAMVTWMIFWMQKAGRTMRSNLQ
                     GGVDRALADGGLWALVAVGFVSVAREGIETTLLLWSMVQSSGDAPLALPGALLGLAVA
                     VVIGWLITRGAVRLDLRRFFAWTGGFLVIVAAGVLAYAVMDLQEAGVLPGPFSALAPV
                     DPGTGVVLTGLAGFPFGWAFDASGVIVPGGVWAVLLQAIVGFMPHMTWLQVIVWALYI
                     IVVGTLFIRGIRTGRPRAHAAPVQTSSLTSTSPGAA"
     gene            6903..7901
                     /locus_tag="VN041_03470"
     CDS             6903..7901
                     /locus_tag="VN041_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWU28137.1"
                     /translation="MSDLNLPVLDLSRLDAGEEEAAAFRADLRAATHDVGFFYLTGTG
                     LPTGLEQRLHRAARDFFALPDEDKLAIENVKSPHFRGYTRIGGERTQGAVDWREQIDI
                     GPERDAVAGGPPYSRLIGPNLWPAAQPELREVVAEWHAALSAISRRLLRAWALALGAE
                     ESYFDDHFGEPSTLIKIVRYPGTHDPLPQQGVGAHKDSGVLTLLWVEPGRGGLQVERD
                     GEWVDAPPVEGAFVVNIGELLEYATGGYLKATNHRVISPAAPADRLSIPFFFNPALDS
                     QLPLIDLPAELAAQARGVTDDPANPIHSLYGENALKSRLRAHPDVAAIHHADLVGA"
     gene            complement(7903..8220)
                     /locus_tag="VN041_03475"
     CDS             complement(7903..8220)
                     /locus_tag="VN041_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU28138.1"
                     /translation="MADSPEHPRLRLDDSFASPIRFSLMAALGEELELDFATLAGILQ
                     VSDSALSKAISSLEQVHYVKVRKGQVGARGRTWVTGTAAGRRAFQAHLEALRAIVDYG
                     TGG"
     gene            complement(8213..9067)
                     /locus_tag="VN041_03480"
     CDS             complement(8213..9067)
                     /locus_tag="VN041_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28139.1"
                     /translation="MEQRPEPGLEPYEPPTAAVAQAYLDESARVAQRREQHIDRRAAA
                     RLLLAEGITFGIYLIVLMLVFPPAEGVNIIVIVAPFIVWTQLVTMLREEYGYQRRGRE
                     QRMRTAVMLILLAVVIGSLGTLMLGVDIPVALRFAPGVLCFVLYGLLAWGEWRHATAE
                     RIVRKRAPFDRGARLTTACIGIAVGAIVACVATPSALIANVVNLLVMLALVVWLSASM
                     LTQGSQIAVAWGPFLWSCLALSGAVIVALLLLARFTSVPLTIWGYVAGGAIALAFVLG
                     AWWGPDRG"
     gene            complement(9215..>9383)
                     /gene="rpsO"
                     /locus_tag="VN041_03485"
     CDS             complement(9215..>9383)
                     /gene="rpsO"
                     /locus_tag="VN041_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWU28140.1"
                     /translation="RIKDLTEHLKEHKHDHHSRRGLFLLVGQRRRLLGYLQDIDINRY
                     RSLIERLGLRR"
BASE COUNT         1461 a         3192 c         3252 g         1478 t
ORIGIN      
        1 gccgcgagcg cgctgggcat gtcccgggtc gccgcccgac ggtatctgga gcacctcgcc
       61 gacgacgggg tggctgtgcg ggaggcgcgc tacggcacgc gcggacggcc ggagacccag
      121 taccgctgga atctccggcc gccacgctga ccgatcagtg cctcactccc cggcgaggcc
      181 tccgagcagg tggccgaagg acttgccctt gcccaggtag gtgtcagggt cgaaggggtc
      241 ggcgtcggtg accggctcct tgcgcgcgat cgcctcggcg agctcgcgcg cgccgcgctc
      301 cacacgggcg ctgagcggtg ccacgtcgtc ggcgttcgca ccccagtcgc tggacgccgc
      361 gaacacgccg gtcgagacgg gctcggcgtg cagataggtg aacagcgggc ggatcgcgta
      421 gtcgatggcc agcgagtggc gcgccgtacc cgcgttcgct ccgatcagaa cgggcttgcc
      481 ggtgagggcg tcggggtcga gcacgtcgat gaacgacttg aacaggcccg cgtagctggt
      541 cgagaagatc ggcgtgacgg cgatgacggc atccgccgac acgacccggt tgatcgcctc
      601 ctccagcggc gccggagcga acccggtgag caggttgttc gtgatgtcat gcgcgaggtc
      661 gcggagctcg atcacgtcca cctgcacgtc gatgtcgcgc tcgcgcagag ccctcgcggt
      721 ctcggcggcc agccgatcgg ccagcatccg cgtcgaggac gggttggaca gaccggacga
      781 gacgacggcg atgcggcgct cgctcatctc acgcctcctt ccgggagagg ccgaacgcgg
      841 cgcctgcggg agccggggcg tcctggtacg gggagcccgc ggtgaggttg tcgccgcggt
      901 tcgcggcggg acgcgcctca cgagccggac cgtcgccgta gaccgccttc acgcgggccg
      961 catgggtcgg agcatccggc accgtcgcgg gacggtcctt cgcgagctcc ttgcgcagca
     1021 cggggacgac ctcctcgccg agcaggtcga tctgctcgag cacggtcttc agcggcaggc
     1081 ccgcgtgatc catgaggaac agctggcgct ggtagtcgcc gaaggtgtcg cgcatggcgg
     1141 cgtagcggtc gatgacctgc tgaggcgagc cgacggtcag cggcgtcatc tcggtgaagt
     1201 cctccatcga cgggccgtgg ccgtagaccg gggcgttgtc gaagtacggg cggaaacggt
     1261 tgacggcgtc ctgcgagttc ttggcgatga acgcctggcc gccgaggccg acgatcgcgg
     1321 tctcgggtgc gccgtggccg tagtggctcc accgctggcg gtacagctcg atcaggcgct
     1381 ggtagtgctc cttcggccag aagatgttgt tcgcgaagaa gccgtcgccg tagtaggcgg
     1441 cctgttcggc gatctccggc gtgcggatgg agccgtgcca gacgaacggc ttgacgccgt
     1501 cgagcggccg aggagtggcg gtgaagccct gcagcggagt gcggaacttg ccctcccagt
     1561 ccacgacgtc cttggtccac agctcgttca gcagcgcgta gttctcgatc gcgagcggca
     1621 ggccctgacg gatgtcctga ccgaaccacg ggtacaccgg cccggtgttc ccgcgcccca
     1681 tcatcaggtc catgcggccc ccggagaggt gctgcagcat cgcgtactcc tcggcgatgc
     1741 gcaccgggtc gttcgtggtg atcagcgtcg tcgaggtcga gacgatcagc cgctcggtct
     1801 gcgcggcgat gtacgccagc agcgtggtgg ggctggagga gaagaagggc gggttgtggt
     1861 gctcaccgat ggcgaagacg tcgagaccga cctcttccgc gtgcatggcg atcgtcaccg
     1921 ccgcctggat ccgctcacgc tcgctgggcg tgatgcccgt cgtcgggtcc tgcgtgatgt
     1981 cgctgaccga catgatgccg aactgcatac cggctgtctg ggtgctgttc tgagcgtcca
     2041 tgtgttgatt ctccactcgg gatgcggtgg catccgtttc tattcaaatg aatgtacttg
     2101 gtggaacagc gcaggcgcaa ggatattccc gcaggcgccg gagaagtccg cccgggtagc
     2161 ctcgaaggat gagcagtgcc gatcagcatc cgcccacgac gacgccgacg gggacgaacc
     2221 gcgggcggcg ccgcgtgccg ttctgggaca acgcccgctt cgcgtgcgtc gtgctggtgg
     2281 tgatcgggca cggcacgcag cgcctgatct acgactccga catctcctac gcgttctacc
     2341 tctggatcta cgcgttccac atgccggcgt tcgcgatcat ctccggctac ttctccaagt
     2401 cggcgagccc cacgaagcgt cagatggctc gcgtgatcac cgacatcctg gtgccctacg
     2461 tgatcttcga gggactgtgg accctcacca aatggctggt cgagggacgc gcggatccga
     2521 acctcacaca gccgtcatgg accctgtggt tcctgctggc actcggaatc ttccgcctgg
     2581 tcctgcccta tctcgctctg ctgcgctggc cactggcctg ggcgctggcg atctccgtgg
     2641 gcgtcggcta tctcagcaac gtcgacagca ccttctcgct ctcacgcacg ctgggcctga
     2701 tgttcttctt cacgctcggc tggtggctgc gcgaacgcga cgtcgtgcgc cggttcagcc
     2761 tgatcgacta ccgcccgtgg tggctgcgga tctgcgctgt cgccgtgctc gccgtgtggg
     2821 ctttcgtgtg ctggcgctgg ctggcgttct gggacgacgt cgacctgcgc ttctggttct
     2881 tctacgacga ctcgtactcc gggctctccg ccgatcagtg gtgggcggga ggcatccgcc
     2941 tgggtctgat ggcgctggcg atcctgctga gcgcgtgctt cttcgtgctg ctcccccgtc
     3001 gcgcgcagtg gtggaccacc ttcgggcagt acacgatgta tgtctacctg ctgcactcgt
     3061 tcgtgctcta cccgttccgc gaatcgggtc tgctgcgcgg gctcgacccc acgtggatct
     3121 ggctcccgct gatcaccgtg ctcgctgtcg ccatcgcgtt cctgctggcc tcgaagccgg
     3181 tgcggagggt gttccgtccg ctgatcgagc cgcgtccgaa gtggatgttc acggatgccg
     3241 cactgtcatc cggatcggac caccgcagtg atcccaccgg ctcgcgtcgc ggataggcgt
     3301 cacgcgtcgc ggaacagcgc ctcgcccacg taggaaccgg agcgcgcgcc ggcgggaacg
     3361 gcccagatgc cggagccgac gtgccggatg tactcgttca gcagatccgt ggagagcttc
     3421 cgctggagcg cgatgaactg ctccggatcg cgctggtagg cgaggaagaa gagccccgca
     3481 tcaaggcgcc cgaggctgtt gttgccgccc acgtagttga agccgcggcg caggatgcgg
     3541 gtgccgccgt tgctgtcggg atgcgcgagc cgcacatggc tgtgcgcatc gagcccgggc
     3601 agcttgggcg cggtcccctc ggtgccgccg ctcaggggtg cgccctcgcc cttgtcgcgt
     3661 ccgaagatgc gctcctgctc gccgagccgc acccggtccc aggtctcgat gagcatcgcg
     3721 atcttgcgcg cgacgaggta cgagccgccg gcgagccagg ccggatcgtc gccgtccgcg
     3781 acccacacgt gatcgtcgag cgccttggca tcgtcggcga ggatgttggc ggtgccgtcc
     3841 ttgaacccga acaggttccg cggggtctgc tgctccgccg tcgtgcgcga ggtcttgccg
     3901 aagccgagct gcgaccagcg cagcttggcg cgcccgaacg cgatgcggct gaggttgcgg
     3961 atcgcgtgca cggcgacctg cggatcgtcg gcgcagacct gcacgcacag gtcgccgtcg
     4021 gagagcgtcg gatcgagatc ctcgccgaga aatgccggaa gccgctgcag cttcgcgggc
     4081 cgacggtcgc ggatgccgta gcgatcaccg tcctcgttct cgaagagcgt cggaccgaag
     4141 ccgaaggtga cggtcaggga gctcgccgga agccccagcg cctccccggt gtcatccgga
     4201 ggcgcctcgg acggtccgcc cacggcgccc gtcgcactga cctcgagccc ctgcgtcatc
     4261 cgggaggccg cgtacgacca gtcctgcagc agttcgatga ggtcggcgcg gtcgctgcgg
     4321 ggcatcatgt cgaacgccgc gaaatgcaga tgctcctgca ccggggtggt gatgccggcc
     4381 tggtgggtgc cgaagaacgc atgcactccg gatgctgtct cgtcccgccg ctgggcggcg
     4441 gcgagcgcat agcctccgcc accggccaga gccagggcgg cggctccccc gcccgctgcg
     4501 agacccagca ggccccgcct gctcagtccg ggccgcttcc gccccgcttc gacatcctcg
     4561 gtcatcggcg gatcaggcga gagccgtgct ggtcagcttc gacagcggct cggcgagggc
     4621 gttgatcaga tcgatcagct cgcgcttgtc ggcgtcgttc agctgcgtga acgggacgaa
     4681 cccggcatcc agcgatccct gcgcggccag cgccttctcc aggtccgcgt acccggtgga
     4741 gatctcctcc acgagcgcct tgccgtccgc gcccttcgag gcgacgaggt cgctgacgag
     4801 cgagaacgcc atcttcgaac cctcgacgtt ggcggcgaag tcgtagaggt cggtgtgcga
     4861 ccaccagtcc tcctcgcccg agatcttgcc cgtggcgacc tcgtccagca gcgcgaccgc
     4921 accgttggag atgccggcga tgccctggtc gttcagcgcg gtggtgaagt ccttcgagtg
     4981 gacgtagtcg tacagttcct gcacgtcgga gatcagcaga tcaccgaact cgccccgctg
     5041 ctcaggcgtc gacggcgccc agtccttcca cgccgacgtc tcgccgtcgg agttcagcgc
     5101 gtcctgggcg ggctgccaca ggtccttctc caggcggtgg aagccggtcc agtccaggcc
     5161 gtcggcgacg gcatccacct cgcggaagtc gatgcgggga tcgagatcgc cgagcgcttc
     5221 ggcgaccggc tcgatgcgct cgtagtacgc gcgcgtgttc gggaacagct cgcgcgcctt
     5281 cgcgtcgtcg ccggactgat aggcgttcac gagttcctgc accgccggga ccagctgtcc
     5341 gacctgatcc ttcacgaacg cggcgtagag gtcgacggcc ttctgcttct gctcggcatc
     5401 cggcccgtcg atggcgacct tgtcgccgct cacggtgaac gcggacttgc cgacgccgtc
     5461 gccgaccatc ccgggcttgc acacggtgaa gtagtcgccc ggctgtgcca cgacggtcat
     5521 ggtgcgggac gcagacggag cgatgttctc cacctcgccg acgatgcgca ggccgtcgga
     5581 tgccaggagg tagaactcgg tgacctggtc tccgctgttg gagatgtcga aggtgagcgt
     5641 gccgctcttc gccgtgtcgg cggagaccgt gcaggcatcg gccgtggact tcacggtgaa
     5701 ggatgcggcc gggtcggcgg tcgccttggc gacgcatccg gtgagggcga tggctgctgc
     5761 cgccagcgcc gcagcggcgc cgagagtgcg ggcggtgagg gtcatgctgc tccaggggat
     5821 gtcgaggtga gggaggaggt ctgaacgggg gcggcgtggg cgcgcggacg accggtgcgg
     5881 atgccgcgga tgaacagggt gccgacgacg atgatgtaca gcgcccacac gatcacctgc
     5941 agccaggtca tgtgcggcat gaaccccacg atggcctgca ggagaaccgc ccagacaccg
     6001 ccgggcacga tgacgccgct ggcatcgaac gcccatccga acgggaatcc ggccaggccg
     6061 gtgagcacga cgcccgtgcc cggatccacg ggggcgagcg cggagaacgg gcccggcagc
     6121 acgccggcct cctgaagatc catcacggcg taggcgagca cgcccgcggc gacgatcacg
     6181 aggaagccgc ccgtccaggc gaagaagcgt cgcaggtcca gacgcacggc gccgcgggtg
     6241 atcagccagc cgatgacgac ggcgacggcg agtccgagca gcgccccggg gagcgccagc
     6301 ggcgcatcgc cggacgactg caccatggac cacagcagca gggtggtctc gatgccctcc
     6361 cgcgcgacgg acacgaagcc gacggcgacc agcgcccaca ggccgccgtc cgccagcgcg
     6421 cggtcgacgc cgccctgcag gttcgacctc atggtgcgtc cggccttctg catccagaag
     6481 atcatccagg tgaccatcgc cacggcgacc agcgacagcg tgccgccgat gatctcctcg
     6541 gcctcggatg tcagggcgta ggcgccgaaa gtgagcacgg cgccgatgcc cagcgccagg
     6601 gcgatcgcca gtccgatgcc gatccacatc cggcccagga cgtcggcgcg cccgaggcgc
     6661 cggacgtagg ccacgaggat gccgacgacg agagccgcct ccaggccttc gcgaaggccg
     6721 atcaggagag cgggaagcat gactgcgagc acgagcgggg tgttctccgt gtgtcgagga
     6781 tcggtaaggg gtgccttacc taaccgagcc tagcattacg cccgccgggc gcccgtcgcg
     6841 gggccgggta aaacagcgca acattcgcgc gaccgcacag tcatccgcat taccgtcgac
     6901 gcatgtccga cttgaacctt cccgtcctgg acctctcccg cctcgatgcc ggagaggagg
     6961 aggccgccgc gttccgagcc gatcttcgtg cggcgaccca cgacgtcggc ttcttctacc
     7021 tgaccggcac gggcctcccc accgggctgg agcagcgcct gcaccgggct gcgcgggact
     7081 tcttcgcgct tcccgacgag gacaagctcg cgatcgagaa cgtgaagagc ccgcacttcc
     7141 gcgggtacac ccggatcggc ggcgagcgca cgcagggcgc ggtggactgg cgcgagcaga
     7201 tcgacatcgg acccgaacgg gatgctgtgg ccggtgggcc gccgtactcc cggctgatcg
     7261 gcccgaacct gtggccggcc gctcagcccg agctgcgcga ggtggtcgca gaatggcatg
     7321 cggccctctc cgcgatctcc cgtcggctgc tgcgcgcgtg ggcgctggcg ctgggcgccg
     7381 aggagtcgta cttcgacgac cacttcgggg agccctcgac gctgatcaag atcgtgcgct
     7441 acccggggac gcacgacccc cttccgcagc agggcgtcgg cgcccacaag gacagcggtg
     7501 tgctgaccct tctctgggtc gagcccggcc gcggcggcct gcaggtcgaa cgcgacggcg
     7561 agtgggtcga cgccccacct gtcgaaggcg cgttcgtggt caatatcggg gagctgctgg
     7621 agtacgccac cggcggatat ctgaaggcga cgaaccaccg ggtcatctcc cccgcggccc
     7681 ccgccgaccg cctgtcgatc ccgttcttct tcaaccccgc gctggactcg cagctgccgc
     7741 tgatcgacct ccccgcggag ctggccgccc aggcccgcgg cgtcaccgac gacccggcca
     7801 acccgatcca cagcctctac ggcgagaacg cgctcaagtc acggctgcgg gcgcatccgg
     7861 atgtcgccgc aatccatcac gcggacctgg tgggggcctg agtcacccgc ccgtaccgta
     7921 gtcgacgatc gcgcgcagcg cctcgaggtg cgcctggaac gcccgacggc cggctgctgt
     7981 gccggtgacc caggtgcgcc cgcgagcacc gacctgcccc ttgcggacct tcacgtagtg
     8041 cacctgctcg agcgaggaga tcgccttgct gagcgcggag tcggagacct gcaggatgcc
     8101 ggccaaggtg gcgaagtcga gctcgagttc ctcgccgagc gctgccatca acgagaaccg
     8161 gatgggactg gcgaaactgt cgtcgagtcg gagccgcgga tgctcggggc tgtcagccac
     8221 ggtcgggccc ccaccaggct ccgagcacga acgccagcgc gatcgcacca cccgcgacgt
     8281 agccccagat ggtcagcgga acggaggtga accgcgccaa cagcagcagc gcgacgatca
     8341 ctgcgccgga gagggcgaga cagctccaca ggaacggccc ccatgccacc gcgatctggg
     8401 atccctgggt cagcatcgag gccgacaacc acaccaccag ggcgagcatc accaggaggt
     8461 tgacgacgtt ggcgatgagc gcggagggtg tcgccacgca ggcgacgatc gccccgaccg
     8521 cgatcccgat gcaggccgtg gtcaaccgcg ccccgcggtc gaagggcgcc cgcttccgga
     8581 cgatccgctc ggcggtcgca tgtcgccact cgccccacgc gaggaggccg tacagcacga
     8641 agcacaggac cccgggcgcg aacctcagag cgacggggat gtcgacgccc agcatgagcg
     8701 tgccgagtga tccgatgacg actgcgagca ggatgagcat caccgccgtc cgcatccgct
     8761 gctcgcgacc tcggcgctgg tagccgtact cctcccggag catcgtcacc agctgagtcc
     8821 agacgatgaa cggcgcgacg atgacgatga tgttgacgcc ttcggcgggt gggaacacga
     8881 gcatgagcac gatgaggtag atcccgaacg tgatcccctc ggcgagcagg agccgcgcag
     8941 cggcgcggcg gtcgatgtgc tgctcgcgtc gctgagccac acgcgccgat tcatcgagat
     9001 acgcctgcgc gacggctgcg gtcggcggct cgtagggctc cagccccggt tccggtctct
     9061 gctccatgac atccccccac tttccaccag gaaacctaac tactttccta cgggaaaccg
     9121 aacggctccg tcaaccccgg acacagcgag agccgcccca caccgtgggg cggctctcca
     9181 agaatgttct gcgcgatata cgcggcagcg gtgcttatcg acgcagaccg aggcgctcga
     9241 tgagcgagcg gtaacggttg atgtcgatgt cctgcaggta acccagcagg cgacggcgct
     9301 gaccgacgag caggaacagg ccacgacgcg agtggtggtc gtgcttgtgc tccttgaggt
     9361 gctcggtgag gtccttgatg cgc
//
LOCUS       DATMSX010000111         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_115275,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000111 DATMSX010000000
VERSION     DATMSX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115275"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VN041_03490"
     CDS             <1..509
                     /locus_tag="VN041_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28141.1"
                     /translation="WLLTLPAAGIVGALAAVVVRWLGGWGVAIDAVLAVAIIVGLFLR
                     SRRNAVTHANAMSDVAESGHAVELPEVPGPTRRTSRIELMRALERAERKADDAERAAK
                     RAKKLKKSGGKPSEIKAARKAAERAAQKYAEAEKAAAEWEELSASRRARAKLAEWDLG
                     VEDQEEAR"
     gene            506..847
                     /locus_tag="VN041_03495"
     CDS             506..847
                     /locus_tag="VN041_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWU28142.1"
                     /translation="MSVEIDWLAFLSVFAAALIGAVAIVAFYALGLRMLVRAGRVPVV
                     TPAEFTDAITVLSEKEIKRAAKQAQKAARKSPLTEGQKTLAFVAALGCFALCAAAVIG
                     GILLIVLGGGH"
     gene            900..1817
                     /locus_tag="VN041_03500"
     CDS             900..1817
                     /locus_tag="VN041_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HWU28143.1"
                     /translation="MIDTPVFGRHQPPSHTIIHLSDPHFVAGGARHSGGADSDGNLAH
                     TLATIRRVDRHPSAIVVTGDLTDFGEPDAYRRVRAAIEPVAAELGCPIVWVAGNHDER
                     PALRAELLDEPASEEPITGVWDLDGLRLIALDSSVPGWHHGDIDAAQRDWLAGVLAEP
                     APHGTLLALHHPPLPAHVPLFDILELQHQDELADVIRGTDVRGILAGHLHYSCSGMFA
                     GVPVSVASSTCYTMNLGRPAAEVNGMDAAQAFQLVHVRPDVITHTVVPVAEAPTGDYF
                     SEEWIRRMAALSPEGRLEAFSRKPPSAPA"
     gene            1868..3328
                     /locus_tag="VN041_03505"
     CDS             1868..3328
                     /locus_tag="VN041_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HWU28144.1"
                     /translation="MAADAPALTRTETDSIGSLEIPADAYYGVHTLRAEQNFPITKRP
                     ISVYPDLIRGLAMVKQASARANKEIGVLDAERADLIDAASQRIIDGEFHDQFVVGVIQ
                     GGAGTSTNMNANEVITNVALEMAGHEKGDYAYLSPIDHTNRSQSTNDVYPTAVKIGLS
                     LNLRSLLDELDLLRQSFLSKASEFHDVLKVGRTQLQDAVPMTLGQEFHGFATTLGEDH
                     SRLTENASLMFEINMGATAIGTGITTHAGYAPTVLKHLREISGLDLQTAGDLVEATSD
                     TGSFMSFSSSLKRNAIKLSKICNDLRLLSSGPQAGLGEINLPAMQAGSSIMPGKVNPV
                     IPEAVNQVAFAVAGADVTVTMAAEGGQLQLNAFEPVIAHSIFQSITWMRQAMWTLRVN
                     CVDGITANRERLGAMVGASVGVVTALTPFIGYAASAALAKTALLTNRNIADLVVEAGL
                     MTREEVMKQISPARLSGLETVTSAIPVIAAEDLPEN"
     gene            complement(3396..4265)
                     /locus_tag="VN041_03510"
     CDS             complement(3396..4265)
                     /locus_tag="VN041_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU28145.1"
                     /translation="MRFAHLSARDSESLQLAVIDGDEAILVSDLLSPGPKTLQELIVG
                     GDEMLEALRTAVAAGTAPRHPLNGFSFGSAVVDPPAILAVGLNYSAHSSELGLKTDTA
                     PTVFTLWPNSLAGHGQTTSWPRTLSESVDYEAELGVIIGQPAKDVSEEDALSTVWGYT
                     VVNDITARDIQFAEAQWCRCKSFDGFTPTGPVVVTADEIPDPQALHIWTVVDGHTVQD
                     ASTEQMVRTVATLVAHLSSSVTLLPGTLISTGSPGGAGYSRDPQIFLRDRSTVTVGIE
                     GIGELTTHCRILD"
     gene            4325..>4540
                     /locus_tag="VN041_03515"
     CDS             4325..>4540
                     /locus_tag="VN041_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28146.1"
                     /translation="MSFGGQPPAYQPPPPSEQPAPEGQQPVYGQPPAPVYGQPPAPVY
                     GQGQPAYGQPPAPAQSYPSAPAYGQPPA"
BASE COUNT          721 a         1617 c         1530 g          672 t
ORIGIN      
        1 gatggctgct cacgcttccc gcagccggca tcgtgggcgc tctggcggcc gtcgtggtgc
       61 gctggctggg tggctggggc gtcgcgatcg acgcggtgct cgccgtcgcg atcattgtcg
      121 ggctgttcct gcgctcccgg cgcaacgccg tcacccacgc gaacgcgatg agcgacgtgg
      181 ctgagtccgg ccacgccgtc gagcttcccg aggttcccgg tcccacgcgc cgcacgagcc
      241 gcatcgagct gatgcgcgcg ctcgagcggg cggagcgcaa ggccgatgac gcggagcggg
      301 ccgccaagcg ggccaagaag ctcaagaagt ccgggggcaa gcccagcgag atcaaggccg
      361 cacgcaaggc cgccgagcgc gccgcccaga agtacgcgga ggctgagaag gccgccgcgg
      421 agtgggagga gctcagcgcc agccgccgcg cccgcgcgaa gctcgccgag tgggatctgg
      481 gcgtcgagga tcaggaggaa gcccgatgag tgtcgagatc gactggctgg ccttcctcag
      541 cgtgttcgcc gccgctctga tcggtgctgt cgccatcgtc gcgttctacg cgctgggcct
      601 gcggatgctt gttcgcgccg ggcgggtgcc cgtggtgacg cccgcggagt tcaccgacgc
      661 catcacggtg ctctccgaga aggagatcaa gcgggcggcc aagcaggcgc agaaggccgc
      721 gcggaagagc ccgctcaccg aaggtcagaa gaccctcgcg ttcgtcgcgg cgctcggatg
      781 cttcgcgctg tgcgccgccg cggtcatcgg cggcatcctg ctcatcgtcc tgggcggcgg
      841 gcactgaggt atcgcgtccg gacggcgcgg acgtcggcgg acgaccctat gctgggcgca
      901 tgatcgacac accggtgttc ggacgccatc agccgccgtc gcacacgatc atccacctga
      961 gcgacccgca cttcgtcgcc gggggagctc ggcactccgg cggcgccgac agcgacggga
     1021 acctcgcgca cacgctcgcg acgatccggc gggtcgatcg gcatccgtcg gcgatcgtcg
     1081 tcacgggcga cctcaccgac ttcggcgagc cggacgcgta ccggcgcgtc cgcgcggcga
     1141 tcgaacccgt cgcggccgaa ctcggatgcc cgatcgtgtg ggtcgcggga aaccacgacg
     1201 agcgacccgc gctgcgcgcg gagctgctcg acgagcccgc gagcgaggag cccatcaccg
     1261 gtgtctggga cctcgacgga ctgcgcctga tcgcgctgga cagcagcgtc cccgggtggc
     1321 accacggcga catcgacgcc gcacagcggg actggctcgc cggcgtgctg gcggagccgg
     1381 ccccgcacgg cacgctgctg gccctgcacc acccgccgct gcccgctcac gtgcccctgt
     1441 tcgacatcct cgagctgcag catcaggacg agctcgccga cgtcatccgc ggcacagacg
     1501 tgcggggcat cctcgcaggc cacctgcact attcgtgctc gggcatgttc gcgggggtcc
     1561 cggtgagcgt cgcgtcctcg acctgctaca cgatgaacct gggacggccc gccgccgagg
     1621 tgaacgggat ggatgccgcg caggccttcc agctcgtgca tgtgcgaccc gacgtcatca
     1681 cccataccgt cgtcccggtc gcggaggctc ccaccggcga ctacttcagc gaggagtgga
     1741 tccgccggat ggccgcgctg tcgccggagg gtcgactcga ggccttctcg cgcaagccgc
     1801 cttccgcccc ggcatagaat tgatgcatcc cccgcatccc attcgccacc acccacaagg
     1861 aagtgacatg gctgctgacg cgcctgccct gacccgtact gagaccgact cgatcggaag
     1921 cctggagatc cccgccgacg cctactacgg cgtgcacacg ctgcgggccg agcagaactt
     1981 cccgatcacc aagcgcccga tctcggtgta ccccgatctg attcgcgggc tggcgatggt
     2041 caagcaggcc tcggcccgtg ccaataagga gatcggcgtg ctcgacgccg agcgcgccga
     2101 cctgatcgac gcggcctcgc agcgcatcat cgacggggag ttccacgatc agttcgtcgt
     2161 cggcgtcatc cagggcggtg ccggcacctc gaccaacatg aacgcgaacg aggtcatcac
     2221 caacgtcgcg ctcgagatgg ccggccatga gaagggcgac tacgcctacc tctcgccgat
     2281 cgaccacacc aaccgcagcc agtccaccaa cgacgtctac ccgaccgccg tcaagatcgg
     2341 cctgtcgctg aacctgcgct ccctgctcga cgagctcgac ctgctgcgtc agtcgttcct
     2401 gagcaaggca tccgagttcc acgacgtgct caaggtcggc cgcacgcagc tgcaggacgc
     2461 cgtgcccatg acgctcggcc aggagttcca cggcttcgcg acgaccctcg gcgaggacca
     2521 cagccgcctc accgagaacg cctcgctgat gttcgagatc aacatgggcg ctaccgcgat
     2581 cggcaccggc atcaccacgc acgccggcta cgcgcccacc gtgctcaagc acctgcgcga
     2641 gatcagcggc ctggacctgc agaccgccgg agacctcgtc gaggccacca gcgacacggg
     2701 ctcgttcatg tcgttctcct cgtcgctcaa gcgcaacgcg atcaagctct cgaagatctg
     2761 caacgacctg cgtctgctct cctccggccc gcaggccggc ctcggtgaga tcaacctgcc
     2821 cgccatgcag gccggctcca gcatcatgcc cggcaaggtc aacccggtca tcccggaggc
     2881 cgtcaaccag gtggcgttcg ccgtcgccgg cgccgacgtc accgtgacga tggcggccga
     2941 gggcgggcag ctccagctga acgccttcga gccggtgatc gcgcactcga tcttccagtc
     3001 catcacctgg atgcgtcagg cgatgtggac gctccgcgtc aactgcgtcg acggcatcac
     3061 cgcgaaccgc gagcgcctgg gcgcgatggt gggcgcatcc gtcggcgtcg tcaccgcgct
     3121 gaccccgttc atcggctacg cagcatccgc cgccctcgcg aagaccgcgc tgctgaccaa
     3181 ccgcaacatc gcggacctcg tcgtcgaggc cggtctgatg acgcgcgaag aggtcatgaa
     3241 gcagatctca ccggcccgtc tctctggtct cgagacggtc acttcggcca tcccggtgat
     3301 cgctgcggag gacctgccgg agaactgaac tccggaatgc agaagaggcg gatgctgaca
     3361 gcatccgcct cttcttcgtc tgtgcccgtg gccggtcagt ccaggatgcg gcagtgcgtg
     3421 gtgagctcgc cgatcccctc gatgccgacc gtgacagtgg agcggtcgcg caggaagatc
     3481 tgcgggtcgc gggagtagcc ggcgccgccg gggctgccgg tggagatcag cgtcccggga
     3541 agcagcgtga ccgatgagga caggtgcgcg accagtgtcg cgacggtgcg caccatctgc
     3601 tccgtggacg cgtcctgcac ggtgtgcccg tcgacgacgg tccagatgtg cagagcctgc
     3661 ggatcgggga tctcgtcggc ggtcacgacg acggggccgg tgggggtgaa gccgtcgaag
     3721 gacttgcagc ggcaccactg ggcctcggcg aactggatgt cgcgcgccgt gatgtcgttg
     3781 accaccgtgt agccccagac cgtgctcagc gcgtcctcct cggacacgtc cttggcgggc
     3841 tgcccgatga tgacgccgag ctcggcctcg tagtcgaccg attcgctgag cgtgcgcggc
     3901 cacgaagtcg tctggccatg tcccgccagc gagttcggcc acagggtgaa gacggtgggg
     3961 gcggtatcgg tcttgaggcc cagttcgctg gagtgcgcgg agtagttcag accgaccgcg
     4021 aggatcgcgg gcgggtcgac gaccgcggat ccgaaggaga acccgttcag cggatgccgc
     4081 ggagcggtgc ccgccgccac cgcggtgcgc agcgcctcga gcatctcgtc accgcccacg
     4141 atgagttcct gcagggtctt cgggccgggg gagaggaggt cgctgaccag gatcgcctcg
     4201 tcgccgtcga tgacggcgag ctggagggat tccgagtcgc gagcgctcag atgggcaaaa
     4261 cgcatgcctc tacgctaccg gccaggatgc ccccgccggc tgaggctccc tctaggcttt
     4321 gctcatgagc tttggaggac agcccccggc ctaccagccg ccgcccccgt cggagcagcc
     4381 ggcgcccgaa gggcagcagc ccgtgtacgg tcagccgccg gcccccgtgt acggccagcc
     4441 gcccgcaccc gtgtacggcc aggggcagcc cgcttacggt cagcctcccg ctcccgctca
     4501 gtcgtacccg tctgctcccg cgtacggtca gccgccggct
//
LOCUS       DATMSX010000112         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172157,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000112 DATMSX010000000
VERSION     DATMSX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172157"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VN041_03520"
     CDS             complement(<1..252)
                     /locus_tag="VN041_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28147.1"
                     /translation="MATESRAGRPRASSRETLAEAACELFLEQGYEATSVVDIAQRAG
                     VSRSSFFNYFSSKSDVLWSGLDARIAAASVALAALGRSAD"
     gene            359..1012
                     /gene="lipB"
                     /locus_tag="VN041_03525"
     CDS             359..1012
                     /gene="lipB"
                     /locus_tag="VN041_03525"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWU28148.1"
                     /translation="MLDIRIAGIAPDFVPYLDGWELQRSIHRDVVAGDRPDTLLLLEH
                     EAVYTAGKRTEPQERPQDGTPVVDVDRGGKITWHGPGQLVGYPIVRLPEPMDVVAHVR
                     RMERLLISILKPLGVDGYQVEGRSGVWVRRPLSEDKVAAIGVRVQQGVTMHGFAINCD
                     NSLAGFRGIIPCGITDAGVTTVSEIVGADISPADIVQAVSDAFAAEYATETRKEVAA"
     gene            1009..2001
                     /gene="lipA"
                     /locus_tag="VN041_03530"
     CDS             1009..2001
                     /gene="lipA"
                     /locus_tag="VN041_03530"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWU28149.1"
                     /translation="MSAAHPDGRRLLRLEIRNSETPIERKPEWIRTKAKMGPEYKALH
                     SLVKDEGLHTVCQEAGCPNIFECWEDKEATFLIGGSQCTRRCDFCQIDTGKPADYDTD
                     EPRRVAESVTRMGLRYATVTSVARDDLPDTGAWLNAETVRQIHAQNPNTGVELLANDH
                     GGKPENLNQIFEARPEVFAHNVETVPRIFRRIRPAFTYERSLNVITQGHDAGLITKSN
                     LILGMGEEPDEVVQALQDLHDAGCDIITITQYLRPSPRHLPVARWVKPDEFVEFKAEA
                     ERIGFLGVLAGPLVRSSYRAGRLWAQSMISKGRPIPAHLAHIAESADLGFAQAV"
     gene            complement(2014..2514)
                     /locus_tag="VN041_03535"
     CDS             complement(2014..2514)
                     /locus_tag="VN041_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HWU28150.1"
                     /translation="MAIEHDYFGVLSSGPDGAIFWTERVEFGDQSVTVDLTAPDQEDV
                     SQEALDVAAALISGIEDLDATVRRAMLTEVDDRTSEVTEYILQQQEAYGDQLEEVLVD
                     ISGDAAVDIIRSLCLMSMTILADEHGGTDPFAVMEYALDADATDDVLLVNLADDGSVI
                     SVMSAD"
     gene            complement(2553..3428)
                     /gene="ftsY"
                     /locus_tag="VN041_03540"
     CDS             complement(2553..3428)
                     /gene="ftsY"
                     /locus_tag="VN041_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWU28151.1"
                     /translation="MAEKSWSLGRALRGMFVKPTIDETTWEDLETALITADFGPDITE
                     ALLEELHEKVDRYRTTDPKDLQRMLRETLEERFAKFDTTLKLTERPAVVLVVGVNGVG
                     KTTTIGKFTKFLRGFERSVVVGGADTFRAAAVEQLATWAQRGGAAMVRPQHEGQDPAS
                     VAFQTVEYAKQHGTEIAIIDTAGRLHTKGGLMDELSKVRRVVEKLAPISEVLLVLDAT
                     TGQNGVMQAEAFLQHAGVTGLVITKLDGSAKGGFILAVQERTGIPVKLLGQGEGIDDL
                     TGFTPHVFVSALVGA"
     gene            complement(3464..>4534)
                     /locus_tag="VN041_03545"
     CDS             complement(3464..>4534)
                     /locus_tag="VN041_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU28152.1"
                     /translation="PICRAQREAASAVADEVPRVLDSIDRSVTEARLALAAAEAERSA
                     HNEELTGLRAQEGSLRERLAGLTESVHGLELQIHEKKLHLSSLLERVASELALDEDIL
                     IAEYGPDQPVPRDRDLDGRSVEEQDQDAAIPFDRRIQERRLKDAERKLAQLGRVNPLA
                     LEEFAALEQRHAFLTEQLADLTKTRQDLLAIIADLDERMQVIFASAFEDTKQAFGEVF
                     PLLFPGGSGSISLTDPDDMLTTGIEVAVRPVGKKIERLSLLSGGERSLAAVALLVAIF
                     KARPSPFYILDEVEAALDDANLGRLLTVFEQLRENSQLLVITHQKRTMEIADALYGVS
                     MRQDGVSAVVGQRVGDRAAASA"
BASE COUNT          733 a         1594 c         1500 g          707 t
ORIGIN      
        1 gtcggcgctc cggccgagcg cggccagcgc gacggaggcc gcggcgatcc gggcatccag
       61 gcccgaccag agcacatcgc tcttcgagga gaagtagttg aagaagctcg accggctgac
      121 gccggcacgc tgcgcgatgt cgacgaccga ggtcgcctcg tacccctgct cgaggaagag
      181 ctcgcatgcc gcctcggcca gcgtctcacg cgacgacgcc ctcggcctgc ctgctcgcga
      241 ctcggttgcc atgtccgtca gcgtagcgtc atgccgatgc gcgacaagcc ctgcagacag
      301 gcgtaggctg taccgagctg ctttcttgca ctgcatccaa caaccattgg gagcgatcat
      361 gctcgacatc cggatcgccg gtatcgcacc ggacttcgtc ccctacctgg acgggtggga
      421 gctgcagcgc agcatccacc gcgacgtcgt cgcgggcgac cggcccgaca cgctcctgct
      481 gctcgagcac gaggccgtct acacggccgg caagcgcacc gagccgcagg agcgccccca
      541 ggacggcacg cccgtggtcg atgtcgaccg cggtggcaag atcacctggc acggccccgg
      601 ccagctcgtc ggctacccga tcgtccgcct gcccgagccc atggacgtcg tcgcgcacgt
      661 gcgccgcatg gagcgcctgc tgatcagcat cctgaagccc ctcggtgtcg acgggtacca
      721 ggtcgagggc cgcagcggcg tgtgggtgcg caggccgctg tccgaggaca aggtcgccgc
      781 gatcggggtg cgcgtgcagc agggcgtgac catgcacggc ttcgcgatca actgcgacaa
      841 ctcgctggcc ggattccggg gcatcattcc gtgcggcatc actgatgccg gggtcaccac
      901 cgtcagcgag atcgtcggcg ccgacatctc ccccgccgac atcgtgcagg ccgtctccga
      961 cgccttcgcc gctgagtacg ccaccgagac ccgcaaggag gtggccgcat gagcgccgcc
     1021 caccccgacg gacgcaggct gctgcgcctg gagatccgca actccgagac cccgatcgag
     1081 cgcaagcccg agtggatccg caccaaggcg aagatgggcc ccgagtacaa ggcgctgcac
     1141 tcgctggtga aggacgaggg tctgcacacg gtctgtcagg aggccggctg ccccaacatc
     1201 ttcgaatgct gggaggacaa ggaggcgacg ttcctgatcg gcggctcgca gtgcacccgc
     1261 cgctgcgact tctgccagat cgacaccggc aagcccgccg actacgacac cgacgagccg
     1321 cgtcgcgtgg cggagagcgt cacccggatg ggcctgcgct acgccacggt caccagcgtc
     1381 gcacgagacg atctgcccga cacgggcgcg tggctgaacg ccgagacggt gcgccagatc
     1441 cacgcgcaga acccgaacac cggggtcgag ctcctcgcca acgaccacgg cggcaagccg
     1501 gagaacctga accagatctt cgaggcccgc ccggaggtct tcgcgcacaa cgtcgagacg
     1561 gtgccgcgca tcttccgccg catccgcccc gcgttcacct acgagcgctc actgaacgtc
     1621 atcacgcagg gccacgacgc gggcctgatc acgaagtcca acctcatcct cggcatgggc
     1681 gaagagccag acgaggtcgt gcaggcactg caggacctgc acgacgccgg ctgcgacatc
     1741 atcaccatca cgcagtacct gcgaccgtcg ccgcggcacc ttcccgtggc ccgctgggtc
     1801 aagcccgacg agttcgtgga gttcaaggcc gaggccgagc ggatcggctt cctcggcgtg
     1861 ctcgccggcc ccctggtccg gtcgtcctac cgggccgggc ggctgtgggc gcagtcgatg
     1921 atctcgaagg gccgcccgat ccccgcgcat ctcgcgcaca tcgcggagag cgcagacctg
     1981 ggcttcgctc aggcggtctg acgcttcgac aggctagtcg gcgctcatca ccgagatgac
     2041 cgagccgtcg tcggcgaggt tcacgagcag cacatcgtcg gtggcatccg cgtccagcgc
     2101 gtactccatg acggcaaacg gatcggtccc gccgtgctcg tcggcgagga tcgtcatgct
     2161 catcaggcac agcgagcgga tgatgtcgac cgcggcgtcg ccgctgatgt cgaccagcac
     2221 ctcctcgagc tgatcaccgt aggcctcctg ctgctgcagg atgtactcgg tgacctcgct
     2281 ggtgcgatcg tcgacttcgg tcagcatggc gcggcgcacc gtggcatcca ggtcctcgat
     2341 gccggaaatc agagcagcag cgacgtcgag cgcctcctgg gagacgtcct cctggtcggg
     2401 ggcggtcaga tcgacggtca cggactggtc accgaactcg acccgctcgg tccagaagat
     2461 ggcgccgtcg ggccccgagg agaggactcc gaagtaatcg tgttcgatcg ccatatcgct
     2521 atgaaaccag cctctccgcc cgtgcggtag tcctacgcgc cgacaagggc cgacacgaag
     2581 acgtgcgggg tgaaaccggt gagatcgtct atgccctcgc cctgcccgag gagcttcacc
     2641 gggatgccgg tgcgctcctg cacggccagg atgaagccgc ccttcgcgga gccgtcgagc
     2701 ttcgtgatca cgagccccgt cacgcccgcg tgctggagga acgcctccgc ctgcatgacg
     2761 ccgttctggc ccgttgtggc gtccagcacg aggagcacct cgctgatggg ggcgagcttc
     2821 tccacgactc ggcgcacctt cgacagctcg tccatcaggc cgcccttggt gtggagccgg
     2881 ccggcggtgt cgatgatcgc gatctcggtg ccgtgctgct tggcgtactc caccgtctgg
     2941 aaggcgacgg atgcaggatc ctgaccctcg tgctggggac ggaccatcgc cgcaccgccg
     3001 cgctgcgccc aggtcgccag ctgctcgacg gcggccgcac ggaaggtgtc ggctccgccg
     3061 accaccacgc tgcgctcgaa gccgcgcagg aacttggtga acttgccgat ggtcgtggtc
     3121 ttgccgacgc cgtttacccc gacgaccagc accacggccg gacgctcggt gagcttgagc
     3181 gtggtgtcga acttcgcgaa ccgctcctcg agcgtctccc gcagcatccg ctgcagatcc
     3241 ttcggatcgg tcgtgcggta cctgtcgacc ttctcgtgca gctcctccag cagggcctcg
     3301 gtgatgtcgg ggccgaagtc cgccgtgatc agcgcggtct ccaggtcctc ccatgtcgtc
     3361 tcatcgatcg tgggcttgac gaacatgccg cgcagcgcgc gaccgaggga ccaggacttc
     3421 tccgccatgc ctccagccta tccaggcgac ggccgaccgg ttctcaggcg gatgccgcgg
     3481 cgcggtcgcc cacgcgctgg ccgaccaccg cggagacgcc gtcctggcgc atcgagacgc
     3541 cgtacagggc atcggcgatc tccatcgtgc gcttctgatg ggtgatcacg agcagctgcg
     3601 agttctcgcg cagctgctcg aacaccgtca gcagccgtcc gaggttggcg tcgtcgagcg
     3661 ccgcctccac ctcgtcgagg atgtagaacg ggctgggccg cgccttgaag atcgcgacca
     3721 gcagcgcgac cgcggccagg gaccgctctc cgcccgagag cagggagagc cgttcgatct
     3781 tcttgccgac cggacgcacc gcgacctcga tgccggtggt gagcatgtcg tcggggtcgg
     3841 tcagcgagat gctcccggat ccaccgggga agagcagcgg gaacacctcg ccgaaggcct
     3901 gcttggtgtc ctcgaacgcg ctggcgaaga tcacctgcat ccgctcgtcg aggtcggcga
     3961 tgatcgccag caggtcctgc ctggtcttcg tcagatcggc gagctgctcg gtgaggaacg
     4021 catgccgctg ctcgagcgcg gcgaactcct ccagagcgag aggattgacc cggccgagct
     4081 gagcgagctt gcgttcggcg tccttcagcc gtcgctcctg aatgcgccgg tcgaacggga
     4141 tcgcggcgtc ctggtcctgt tcctccacgc ttctgccatc aagatcgcga tcgcgcggga
     4201 ctggctgatc cggcccatat tccgcgatca gaatatcttc gtcgagggcg agctcggagg
     4261 ccacgcgctc gagcaggctc gagaggtgga gcttcttctc atggatctgc aactcgaggc
     4321 cgtgcacgct ctcggtgagc cccgccaggc gctcgcgcag cgaaccctcc tgcgcccgca
     4381 ggccggtcag ctcctcgttg tgcgcggagc gctcggcctc agcggcggcg agggccaggc
     4441 gcgcctcggt gacggagcgg tcgatggagt cgagcacccg tggcacctcg tcggcgacag
     4501 cggatgctgc ttcgcgctgc gcccggcaga tcgg
//
LOCUS       DATMSX010000113         7041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172166,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000113 DATMSX010000000
VERSION     DATMSX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172166"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VN041_03550"
     CDS             complement(<1..989)
                     /locus_tag="VN041_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28153.1"
                     /translation="MTNPPTQTYPFDTRPLTDPVDPRAVSEFWRSIRSRYPTSASTIV
                     AWVIIGVVAIMILVPMFGITVIGIIASGSGIGPAGGVFSIPFLIMLVALGAGVPIGIV
                     SMRRKRAEAFRIAQFAAANGMAYEHQVNQPALPGMIFGNGSSRVASSLVRGYSPRFVE
                     FGNYRYTTGSGKNQTTHHWGYVAVKLDVPLPNIVLDALGNNALGSNLPATFSRDQRLS
                     LEGDFDRYFSLYCPRGYEADALYLFTPDIMARFIDHAAQLDVEIVDDWMFLYMQRPAS
                     TTDAATWAWLFGTVGALMTKFDQWARWRDDRLREAQASAMALPAAPPLQPGMPA"
     gene            complement(986..1954)
                     /locus_tag="VN041_03555"
     CDS             complement(986..1954)
                     /locus_tag="VN041_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28154.1"
                     /translation="MEWIADPSPGGWLRERLDEGYATMHGVVPRGFPAYARIFHPAYV
                     RSLPDRAVPTPQEYARMTDAESAALHGAYIDETTTWAETAEAFGTQLHPLAQWQRVVR
                     TPGDGDWRTRNAPDGREFTSPEEGSMPPELLAAIARHLVAHTTTPDAGFAALWEGHGG
                     LLGHFGQTPSRAFLMFGDEPGHEQMLERSIHNPFEDAFRKPRWQPGILSEEISKGARL
                     ELPGRDHVLFSAPPRAFADPAWVLDAPWRDVEAEEHGFSPSAQHPSLIWPDDRAWALI
                     SEIDYDSTIVAGSADLVRAICEDPAIEALPVRENADLTWDADEVNR"
     gene            1984..2343
                     /locus_tag="VN041_03560"
     CDS             1984..2343
                     /locus_tag="VN041_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28155.1"
                     /translation="MTQTSTLPTLDGRTFRMVSSTTSAVDPDAPSIFRYFEQDGVIWG
                     DYTGDTVTIGRFVGTRSGDTIQVSFVHVLAATGEVVTGEGESDIERGDDGMLRLVEHY
                     EMHGAPQLSVCVETAAG"
     gene            complement(2370..2957)
                     /locus_tag="VN041_03565"
     CDS             complement(2370..2957)
                     /locus_tag="VN041_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU28156.1"
                     /translation="MSSTEAEHNKDSGRSAAALDAVSYRILDLLRENGRISIAALAEK
                     VGISRANAYTRVESLMADGVITGFSARVDPARAGLSIGAMIFVTVYPQAWASFRAQIA
                     DMPDVEYCAVTTGEHDAMLLIRATDVSGVHAFSTGVIAQLPEVRTVVSVVVLDEVIRR
                     PYVLPPDLPERNLDAPLGMTQWTPATAGRDALPPR"
     gene            3109..4701
                     /locus_tag="VN041_03570"
     CDS             3109..4701
                     /locus_tag="VN041_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007273042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28157.1"
                     /translation="MLSTLRTSAIALGAVALLALTGCSGGNSANNSSNGSNTSGGSLV
                     IDTAFSVETADPGHTYDPTGNMLAKAMYETLVDFKGSDVSTPIPGLASWKANDTATEF
                     TFTIEDGRVFSDGSPVEAKDVVFSLQRIQGMADAKPNFLLSGVTIKEVDAKTVTFTTE
                     TPLLQLPAILANPALGIVNSDVVKKNGGATDGKDTAQKYLDGASAGSGPFVLGSLDLS
                     SQIVLKKNDKYNGDEKPAYDRVVIRNVTESATQLANLKGGDSMVAMDLNGDQVAGLGD
                     GIKVDSVPSGQTIFLLLNQSKGIAGDLANVKLAEGIRYALDYPKLLELAGAGAVQATG
                     VIPPGFEGALPKGVSQDLAKSKAALAEAGYTGQTLKLQFPNDYPVGGVEFTPLAERIQ
                     SQLKDAGVNVELAPAPFATELDAYVNGKEGFGLWFWGPDYADSANFLPFAPGLKVGLR
                     SGWEASADPEIAGLAAGAASATDPKQREEAFTKFAEEMQAKGPFVPMIVPGRNIATSG
                     KVAGAVYNSVWEMDIAEITPAG"
     gene            4716..5777
                     /locus_tag="VN041_03575"
     CDS             4716..5777
                     /locus_tag="VN041_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28158.1"
                     /translation="MTTVAVRTQAQRKKSPLAGYLLRRAGTSLLLLIGVTVVTFLLTN
                     LVPGDPVSAALGEGASQNPETRAAFIKAQGLDQPLFVQYFIYMGKLLRGDLGVSLVTG
                     QPVTRDLATAVPATIEIAICAIILSLAVSLVLGTLAAYRRGLVTDQIVRIVTLIGLSV
                     PTFWLALVSFYVFFLNLRIAPGSGRVSPSIIPPPRVTGLYTIDYLLDGDGVGWADAMA
                     HLALPVMVLSLVTIGLLTRFIRTSVLEVLASDYVRAARAKGLPALRVIVDYVLRGAAL
                     PVLTVVGVAFGSLLSGTVLVESVFAWPGLGTYAYNSAANLDLPGIMGVGLVVGVIYLV
                     INFVVDLLYGVLDPRVRIA"
     gene            5774..6676
                     /locus_tag="VN041_03580"
     CDS             5774..6676
                     /locus_tag="VN041_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28159.1"
                     /translation="MSTVEPRMPLRLKLRWPRAWRTPLGIIGTVIAVAWIVVAFTAQW
                     WVPYPPNAQSLPRLQAPGVETLLGTDGNGRDIFSRLMTGATVTIPLALTLVVAALVIG
                     TIIGALAGYFGGWIDETLMRITDLFMAFPTVILAMVVAASLGPSLFNAVIAAVVVSWP
                     QYSRVTRSIVLSLRNQNYVIAGRLLGHSPARTLVVDILPNVAGPLLVLATLDIGAAIL
                     LLSGLSFLGLGAQPPMAEWGSMISAAMQNFDAWWLGVFPGLAILTVVLAFNFLGDAMR
                     DVLDPTAEVTHEKQSELAASGAAA"
     gene            6673..>7041
                     /locus_tag="VN041_03585"
     CDS             6673..>7041
                     /locus_tag="VN041_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28160.1"
                     /translation="MSILDIRDLTVDIGRPLVKGLSLQLEAGRIHGLAGESGSGKTLT
                     AMSVLGLLPRHARTGGSIRLGDTELLGLRRRALNRIRGRRIAMVFQDPSSSLHPQIPV
                     GRQLTDHMRVHLGLRGAAARQ"
BASE COUNT         1068 a         2469 c         2395 g         1109 t
ORIGIN      
        1 gccggcatgc cgggctgcag cgggggagcg gcaggcagtg ccatcgcgga tgcctgcgcc
       61 tcgcgcagcc ggtcatcgcg ccagcgcgcc cactggtcga acttcgtcat cagggcaccg
      121 acggtgccga acagccacgc ccaggtggcg gcatccgtcg tcgacgccgg ccgctgcatg
      181 tagaggaaca tccagtcgtc gacgatctcg acgtcgagct gggcggcgtg gtcgatgaag
      241 cgcgccatga tgtcgggcgt gaacaggtac agggcatccg cctcgtagcc gcgggggcag
      301 tacagcgaga agtaccggtc gaagtcgccc tcgagcgaga ggcgctggtc gcgcgagaac
      361 gtcgcaggca ggttgctgcc cagggcgttg ttgccgagcg cgtcgagcac gatgttcggc
      421 agcggcacgt cgagcttgac ggccacatag ccccagtggt gggtggtctg gttcttgccc
      481 gagccggtcg tgtaccggta gttgccgaac tcgacgaacc gcggcgagta gccgcgcacc
      541 agtgaggacg ccacccggct ggagccgttg ccgaagatca tccccggaag ggccggctgg
      601 ttcacctggt gctcgtaggc cattccgttc gcggccgcga actgcgcgat tcggaacgcc
      661 tccgcgcgct tgcggcgcat gctcacgatg ccgatcggca cacccgcgcc cagggcgacg
      721 agcatgatga ggaacgggat gctgaacacg ccgcccgccg gcccgatgcc ggaaccgctg
      781 gcgatgatgc cgatcacggt gatcccgaac atcggcacga ggatcatgat cgcgacgacg
      841 ccgatgatca cccaggccac gatcgtgctc gcggaggtgg ggtagcggga gcggatcgag
      901 cgccagaact cgctgaccgc gcgcgggtcg accggatcgg tgagcggccg cgtgtcgaac
      961 gggtacgtct gcgtcggcgg gttggtcatc ggttcacctc gtcggcatcc caggtcagat
     1021 cggcgttctc cctgacgggc agcgcttcga tggcgggatc ctcgcagatc gcgcggacga
     1081 ggtccgccga gccggcgacg atcgtggagt cgtagtcgat ctcgctgatc agcgcccacg
     1141 cccggtcgtc cggccagatc aggctcggat gctgtgccga cggcgagaag ccgtgctcct
     1201 cggcctccac gtcccgccag ggcgcgtcga gcacccaggc ggggtcggcg aacgcccgcg
     1261 gcggcgcgga gaacagcacg tggtcgcggc cgggcagctc caggcgtgcg cccttcgaga
     1321 tctcctcgga caggatgccg ggctgccaac ggggcttgcg gaacgcgtcc tcgaacgggt
     1381 tgtggatgct gcgctccagc atctgctcgt ggcccggctc gtcgccgaac atcaggaacg
     1441 cccgcgacgg agtctgcccg aagtgcccca gcagtccgcc gtggccctcc cagagcgcgg
     1501 cgaatccggc atccggcgtc gtggtgtgcg cgaccaggtg gcgggcgatc gccgcgagca
     1561 gctcgggcgg catcgagccc tcctcgggcg aggtgaactc gcgcccgtcg ggtgcgttcc
     1621 tggtgcgcca gtcgccgtcg ccgggggtgc gcaccacacg ctgccactgg gcgagcggat
     1681 gcagctgcgt gccgaacgcc tcggcggtct ccgcccaggt cgtggtctcg tcgatgtagg
     1741 cgccgtgcag agcggcgctc tcggcatccg tcatccgtgc gtactcctgc ggcgtcggca
     1801 ccgcgcgatc cggcagcgac cgcacgtagg ccggatggaa gatgcgcgca tacgccggga
     1861 acccgcgtgg caccacgccg tgcatcgtgg cgtacccctc gtccagccgc tcgcgcagcc
     1921 atccccccgg ggaggggtca gcgatccact ccatggcaca acggtacctg ccatgatcta
     1981 tgggtgactc agacaagcac cctccccacg ctcgacggcc gcaccttcag gatggtctcg
     2041 tccacgacct cggcggtcga ccccgacgcg ccgagcatct tccgctactt cgagcaggac
     2101 ggcgtgatct ggggcgacta cacgggagac accgtcacga tcggacgatt cgtcggcacc
     2161 cgcagcggtg acacgatcca ggtgtccttc gtgcacgtgc tggccgcgac aggtgaggtc
     2221 gtcacaggag agggcgagag cgacatcgag cgcggcgatg acgggatgct gcgcctcgtc
     2281 gagcactacg agatgcacgg cgctcctcag ctcagcgtgt gcgtggagac tgcggctggc
     2341 tgatcccggc gggggtgtcc ggtgccgcgt cagcgcggcg gcagggcatc ccgcccggcg
     2401 gtcgcgggcg tccactgggt catcccgagc ggtgcgtcca ggttgcgctc gggaaggtcc
     2461 gggggcagca cgtaggggcg ccggatcacc tcgtccagca ccaccacgct gacgacggtg
     2521 cggacttcgg gcagctgcgc gatcacgccg gtggagaagg cgtgcacccc gctgacatcg
     2581 gtggcgcgga tcagcagcat cgcgtcgtgc tcgccggtgg tgaccgcgca gtactcgacg
     2641 tcgggcatgt ccgcgatctg cgcacggaac gaggcccacg cctgcgggta caccgtgacg
     2701 aagatcatcg cgccgatgga gagcccggcc cgcgccgggt cgacgcgggc gctgaaaccg
     2761 gtgatcacgc catccgccat gagcgattcc acccgggtgt acgcgttcgc gcgcgagatg
     2821 cccaccttct cggccagcgc ggcgatcgag atgcgtccgt tctcgcgcag aagatcgagg
     2881 atgcggtagg agacagcatc cagggcggcg gcacttcgtc cagaatcctt gttgtgttca
     2941 gcctcagtgc tggacattct gcattccaat ctccattgtt cgagcagact ctgtcttcat
     3001 gacgcaatgt cattggacag atcgtcgcac atcatgggaa tctgatcacg gtcgtccact
     3061 gtagggcgat ctggactccc cgaatccacc ctgccaacgg aggtacccat gctgtcgacc
     3121 ctgcgcacgt cggcgatcgc cctcggagcg gtcgcgctcc tcgccctcac aggctgctcg
     3181 ggaggaaact ccgcgaacaa cagcagcaac ggatcgaaca cctccggtgg ttcgctggtg
     3241 atcgacaccg cgttctcggt cgagaccgca gacccgggcc acacctacga cccgacgggc
     3301 aacatgctcg ccaaggcgat gtacgagacg ctcgtcgact tcaagggctc cgacgtctcc
     3361 actccgatcc cgggcctggc gtcgtggaag gccaacgaca cggccaccga gttcaccttc
     3421 acgatcgagg acggccgggt cttctccgac ggctcgccgg tcgaggcgaa ggacgtcgtc
     3481 ttctcgctgc agcgcatcca ggggatggcc gacgccaagc cgaacttcct gctctccggc
     3541 gtgaccatca aggaggtgga tgccaagacg gtgaccttca ccaccgagac gccgctgctg
     3601 cagctgcccg cgatcctcgc gaacccggcc ctcggcatcg tgaactccga cgtggtgaag
     3661 aagaacggcg gcgcgacgga cggcaaggac accgcgcaga agtacctcga cggcgcctcc
     3721 gccggctccg gccccttcgt gctcggctct ctcgacctca gctcgcagat cgtgctgaag
     3781 aagaacgaca agtacaacgg cgacgagaag cccgcctacg accgtgtcgt gatccgcaac
     3841 gtcaccgaga gcgccaccca gctcgccaac ctcaagggcg gcgactcgat ggtcgcgatg
     3901 gacctcaacg gcgaccaggt cgccgggctc ggcgacggca tcaaggtcga ctcggtgccg
     3961 tccggccaga ccatcttcct gctcctgaac cagtccaagg gcatcgcggg cgacctcgcg
     4021 aacgtgaagc tcgccgaggg catccgctac gcactcgact acccgaagct gctcgagctc
     4081 gccggcgccg gcgccgtcca ggccaccggc gtcatcccgc ccgggttcga gggcgcgctc
     4141 ccgaagggcg tcagccagga cctcgcgaag tccaaggccg cgctcgccga agccgggtac
     4201 accggccaga cgctcaagct gcagttcccg aacgactacc cggtcggcgg cgtcgagttc
     4261 accccgctgg ccgagcgcat ccagtcgcag ctgaaggacg cgggcgtgaa cgtcgagctg
     4321 gcaccggcgc cgttcgccac cgagctggac gcgtacgtca acggcaagga gggcttcggc
     4381 ctgtggttct ggggcccgga ttacgccgac tccgcgaact tcctgccctt cgccccgggc
     4441 ctgaaggtcg gcctgcgctc cggctgggag gcatccgccg atcccgagat cgcaggactc
     4501 gccgccggcg ccgccagcgc gaccgacccg aagcagcgcg aggaggcgtt cacgaagttc
     4561 gccgaggaga tgcaggccaa gggcccgttc gtgccgatga tcgtccccgg ccgcaacatc
     4621 gccacctccg gcaaggtcgc aggcgccgtg tacaactccg tgtgggagat ggacatcgcc
     4681 gagatcacgc ccgccggctg aacggaccga ccgtgatgac gacagtggct gtgcggaccc
     4741 aggcgcagcg gaagaagtcg cccctcgcgg gatacctgct gcgccgggcc ggcacgtccc
     4801 tgctgctgct gatcggcgtc acggtcgtga cgttcctgct caccaacctg gtgcccggag
     4861 accccgtctc cgcggcactg ggtgagggcg catcgcagaa ccccgagacc cgcgccgcgt
     4921 tcatcaaggc gcagggcctg gatcagcccc tgttcgtgca gtacttcatc tacatgggca
     4981 agctgctgcg cggtgacctg ggcgtgtccc tggtcaccgg gcagccggtg accagggacc
     5041 tcgccaccgc cgtgcccgcc acgatcgaga tcgcgatctg cgcgatcatc ctcagcctgg
     5101 cggtgagcct ggtgctcggc accctcgccg cgtaccgccg cggcctggtc accgatcaga
     5161 tcgtgcgcat cgtcacgctg atcgggctga gcgtgccgac gttctggctg gcgctcgtca
     5221 gcttctacgt gttcttcctg aacctgcgca tcgcgcccgg ttccggccgg gtctcgccgt
     5281 cgatcatccc gccgccgcgg gtgaccgggc tctacacgat cgactacctg ctcgacggag
     5341 acggggtcgg ctgggcggat gccatggcgc acctggcgct gccggtcatg gtgctgtcgc
     5401 tggtgaccat cggcctgctc acccggttca tccgcacctc cgtgctggag gtgctcgcca
     5461 gcgactacgt gcgcgcggcg cgggccaagg gcctgccggc cctccgcgtg atcgtcgact
     5521 acgtgctgcg tggtgcggcc ctgccggtgc tgaccgtggt gggcgtggcc ttcgggtcgc
     5581 tgctgtcggg caccgtcctt gtcgagtcgg tgttcgcctg gccgggcctc ggcacctacg
     5641 cctacaactc ggcggccaac ctggatctcc ccgggatcat gggcgtcggc ctggtggtcg
     5701 gcgtcatcta cctggtgatc aacttcgtcg tcgacctgct ctacggcgtg ctcgacccga
     5761 gagtgaggat cgcatgagca ccgtcgaacc gcggatgccg ctgcggctga agctgcgctg
     5821 gccgcgcgcg tggcgcacgc cgctcggcat catcggcacg gtcatcgccg tcgcctggat
     5881 cgtcgtcgcc ttcacggcgc agtggtgggt gccgtacccg cccaatgccc agtcgctgcc
     5941 gcggctgcag gcgccggggg tcgagacgct gctcggcacc gacggcaacg ggcgggacat
     6001 cttctcccgc ctgatgaccg gcgccaccgt caccatcccg ctcgcactga ccctggtggt
     6061 cgccgcgctc gtgatcggca cgatcatcgg ggcgctcgcc ggatacttcg gcggctggat
     6121 cgacgagacg ctgatgcgca tcaccgacct gttcatggcg ttcccgaccg tgatcctggc
     6181 catggtggtc gcggcctcgc tcggcccgtc gctgttcaac gccgtgatcg ccgcggtcgt
     6241 ggtgtcgtgg ccgcagtact cccgcgtcac ccgcagcatc gtgctgtcgc tgcgcaacca
     6301 gaactacgtg atcgccgggc gcctgctcgg gcactcgccg gcgcggacgc tggtcgtgga
     6361 catcctcccg aacgtcgccg gcccgctgct ggtgctcgcc acgcttgaca tcggagcggc
     6421 gatcctgctg ctgtccgggc tgtcgttcct cggcctcggc gcgcagccgc cgatggccga
     6481 gtggggatcg atgatctccg cggccatgca gaacttcgac gcctggtggc tgggtgtgtt
     6541 ccctgggctg gcgatcctca cggtcgtgct ggccttcaac ttcctcggag atgcgatgcg
     6601 cgacgtgctc gaccccactg ccgaggtcac tcacgagaag cagagcgagc tggccgcgag
     6661 cggagcggcc gcatgagcat cctcgacatc cgcgacctca ccgtcgacat cggccgtccg
     6721 ctggtcaagg gcctgtccct gcagctcgag gccggccgca tccacggtct ggccggcgag
     6781 tccggctccg gcaagacgct caccgccatg tcggtgctcg gcctgctgcc gcggcacgcc
     6841 cgcacgggcg gctcgatccg cctgggcgac acggagctgc tcgggttgag gcgtcgcgcg
     6901 ctcaaccgca tccgaggccg ccgcatcgcg atggtcttcc aggatccgtc gtcgtccctg
     6961 cacccgcaga tcccggtcgg ccgtcagctc accgaccaca tgcgggtgca cctcgggctg
     7021 cgcggcgcgg cggcgcggca g
//
LOCUS       DATMSX010000114         8559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39564,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000114 DATMSX010000000
VERSION     DATMSX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39564"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN041_03590"
     CDS             <1..223
                     /locus_tag="VN041_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWU28161.1"
                     /translation="GTLAAYAADTLDGDTAAGDSPLLAADLADRVIVTPHLAAQTTQA
                     VDNMGSVSLDDVIAVLRGDEPAHPVRRIA"
     gene            220..1158
                     /locus_tag="VN041_03595"
     CDS             220..1158
                     /locus_tag="VN041_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWU28162.1"
                     /translation="MTVSDSTQPSDYMGFVGVTTGSSSIMKVFPLWADILGLPTRTLV
                     GHDLPMDADPSVYRAMVEQIRDDVHHRGALVTTHKMNVFAAASDLFDELDPFAVSCSE
                     ISSISKRGDRLIGQAKDPITVDLALNDFLPADHFARTGAEVVILGAGGSGTALSWALA
                     ERADAPSLVTVTARSQDKLDHLREVHRQHGTREDLIRYVVTPTPADADAIVAAAPAGS
                     VIANATGLGKDRPGSPLTDAVVFPESSWAWEFNYRGSLEFMHQALAQQDARSLQVIDG
                     WRYFIHGWSQVVADVFGLTLTPEVVEQLAAAAESVR"
     gene            1158..2204
                     /locus_tag="VN041_03600"
     CDS             1158..2204
                     /locus_tag="VN041_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HWU28163.1"
                     /translation="MSGVVRTVLGDVDPAALGRVNYHEHLFQISPLLPGDELDDERAS
                     GLEAASLKASGFETMVDATPFGLGRDPEALARISAATGLAVVATSGRHREAHYAADHP
                     TRSWDASRLAALFAADVQAGMPASDDEPVRNGCTTGEQLHDRDADSVVHPFPDRAGVP
                     AVGPSGEPVRAGMLKGGVDYWRISAFERITLDALGEAHRSTGAPVMVHLEFCTAAHEV
                     LDLLAAEGVASDRVVLAHADRDTDAGLHLSLTERGAFLGYDGFARPRTRSDAELLQLT
                     ASVVAGGGAGRIVLGGDVARATRYIAYGGMPGLAYLGERYVPRLRALVGDEAVDRMLV
                     DNPARLLTLSAPSS"
     gene            2214..2510
                     /locus_tag="VN041_03605"
     CDS             2214..2510
                     /locus_tag="VN041_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWU28164.1"
                     /translation="MSEPTILHATFTARPGEGDRVAALLRDFAEAVRAEPGNVVFDAT
                     RVLDAPDSFFVYEEYVDEAAFRAHLANPAGGPFNAALQELILEPSSVLTFLRRI"
     gene            complement(2807..3802)
                     /locus_tag="VN041_03610"
     CDS             complement(2807..3802)
                     /locus_tag="VN041_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28165.1"
                     /translation="MKYGKKIAVATVAALTVLSLAGCATGKGDTPAGGGDKPAAKDMT
                     IDLVSKGFQHQFWQAVKKGAEEKGKELGVKINFEGPGSETEIEKQLQMLQSSIDRKPS
                     AIAYAALDPEACIPALDAAASANIPVAYFDAPCNSEVGKTLAATDSKVAGALAAEHMA
                     ELIGGKGDVAIVAHSQINSTGVERRDGFVDKIKADYPDIKIVDIQYGDGDHLKSADIT
                     KSMLNAHPDLKGIYATNEGSAVGVANALSELGKTSKDITAIGFDSGAAQIDAIKGGVL
                     AGAITQDPIGIGKTVVQAAYDLANGKSVEKFYDTGSYWYDATNLDDPKISAVLYQ"
     gene            complement(3877..4920)
                     /locus_tag="VN041_03615"
     CDS             complement(3877..4920)
                     /locus_tag="VN041_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28166.1"
                     /translation="MSNSTTTVVQTAVSENTQKGRLGAALRNSVQQGLALGTLIVLFV
                     FFAIFGSNFTSSSNIENILMSTVLVGVLALGTTFVIVTAGIDLSLGFGMTLCSVMMGT
                     FITGWGLPVWVGVLGALATGALIGLINGLNVTFLRLPPFIATLAMMMIAQGLALVISE
                     VKPITFAPETRKQVTSIVQFDPVLTASDGYFASLPMGVLIFVVLAVIAYLTLNKTLLG
                     RYTFAIGSNEEATRLSGVNTRRWTIWVYVFAGLFIGIAGVLLTARLGSAQPTAGAGYE
                     LQAIAAVVIGGTSLMGGRGSIVGTVIGAFIMSVLINGLRSMSIQTEWQYILTGIVILI
                     AVFFDALRNRGKS"
     gene            complement(4920..6422)
                     /locus_tag="VN041_03620"
     CDS             complement(4920..6422)
                     /locus_tag="VN041_03620"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWU28167.1"
                     /translation="MSDPILKVEGISKGFPGVQALKDVHLEVRSGEVLVLVGENGAGK
                     STLMKILSGIYTRDEGTITFEGEPVEFTSPLQAQQLGVTIIHQELNMMPDLTVAQNIY
                     IGREPKVGPFVSERRLNQQTAELLQRLDIHLDPRQKVGELTVAEQQMVEIAKALSFNA
                     KVLIMDEPTSALTDTEVEVLFTLIEQLKAKGTGIVYISHRMDELRRLADRVSVLRDGA
                     YIGSLEKDEISIPKIIEMMVGRAIDEGARPAPRDYTTEPVVLEVQGLSTKSLLRDVSF
                     RLHQGEILGFAGLMGAGRTETARAVIGADPRDGGSIVIGGRERRIRQPEDAVKQGIGY
                     LSEDRKLLGLMLEQDVTFNTVLASLNSYAGALGWMGDRTAKQKTKDYVQQLRVKTPSV
                     NQIVKLLSGGNQQKVVIARWLMRDCDILIFDEPTRGIDIGAKEEIYRLMQQLADSGKS
                     IIVISSELPELLRVANRITVFANGEVTGELRNEEATQEKIMHLAAHGEDS"
     gene            complement(6607..7476)
                     /locus_tag="VN041_03625"
     CDS             complement(6607..7476)
                     /locus_tag="VN041_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWU28168.1"
                     /translation="MNIGCHGLVWTGEFDAAGIRLAVEKTAAAGFDLIEFPLMDPFSF
                     DVEAAKEALAEHGLAVSASLGLSDATDITSADADVVAAGEALLIRAVDVLAELGGTHF
                     CGVIYSAMRKYMMPVTPEGLASSRAVIGRIADHAASRGVQVSLEVVNRYETNVLNTAR
                     QAIGYLAEVDRPNLGIHLDTYHMNIEESDMAAPVLDAAPGLRYVHIGESHRGYLGTGT
                     VDFDTFFKALGRIGYDGPIVFESFSSAVVAEDLSRMLGIWRNLWTDSEELGAHANRFI
                     RDKLVAVDSIRLH"
     gene            7605..>8559
                     /locus_tag="VN041_03630"
     CDS             7605..>8559
                     /locus_tag="VN041_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28169.1"
                     /translation="MPASVKDVAARAGVSASTVSNYLNHPHVLSERTRERVRNAVAEL
                     GFVPNESARQLRAGAGRTIALILLDAWLPYFSELSRGVEDVTREAGWSLFFSNSNRDA
                     AQEQRNIDMFEAHRAQGILIYPLGDVLGRLEGLADKGIRSVVIGPEQPSSSVGSVAFD
                     DRGGGRLAGEHLIGIGRRRIAFLGDPRTVSQSADRLAGLRDAAPDARIDVIATDGLTI
                     DAGLLAAERIIAAPASERPDAVFAANDMLAIGAQTALLRAGIAVPGDIAIVGFDDVDQ
                     ARQALVPLTTIRQPGYEIGRAAASALLRLLEAPSTPAAPVTP"
BASE COUNT         1312 a         2929 c         2878 g         1440 t
ORIGIN      
        1 cggcaccctc gccgcctacg ccgccgacac cctcgacggc gacaccgccg ccggcgacag
       61 cccgctgctg gccgccgacc tcgccgaccg ggtgatcgtc accccgcatc tggccgccca
      121 gaccacgcag gccgtcgaca acatgggcag cgtgtcgctc gacgacgtga tcgccgtgct
      181 gcgcggcgat gagcccgccc accccgtgag gagaatcgca tgaccgtcag cgacagcacc
      241 cagcccagcg actacatggg cttcgtcggt gtcaccaccg gctcctcgtc gatcatgaag
      301 gtgttcccgc tctgggccga catcctcggc ctcccgacgc gcacgctggt cggccacgac
      361 ctgccgatgg acgccgaccc gtccgtgtac cgggcgatgg tggagcagat ccgcgacgac
      421 gtgcaccacc gcggggcgct ggtgaccacg cacaagatga acgtgttcgc ggcggcatcc
      481 gatctgttcg acgagctcga cccgttcgcc gtgtcgtgct ccgagatctc cagcatctcc
      541 aagcgcggcg accggctcat cggccaggcc aaggacccca tcacggtcga cctggccctg
      601 aacgacttcc tgcccgccga tcatttcgcg cgcaccggag ccgaggtcgt catcctgggc
      661 gccggcggct cgggcacggc gctgagctgg gcactcgccg agcgcgccga cgccccctca
      721 ctcgtgacgg tcaccgcgcg ctcgcaggac aagctcgatc acctgcgcga ggtgcaccgc
      781 cagcacggca cgcgcgagga cctgatccgc tacgtcgtca cgccgacgcc tgcggatgcc
      841 gacgcgatcg tcgccgcggc gcccgccggc tccgtgatcg ccaacgcgac cgggctcggc
      901 aaggaccgtc ccggatcgcc cctgacggat gccgtggtct tcccggagtc ctcgtgggcc
      961 tgggagttca actaccgcgg ctcgctggag ttcatgcacc aggcgctcgc acagcaggat
     1021 gcccgctcgc tgcaggtcat cgacggctgg cgctacttca tccacggctg gtcgcaggtg
     1081 gtggccgacg tgttcggcct gaccctcacg cccgaggtcg tcgagcagct cgcggccgcc
     1141 gcggagtcgg tgcgctgatg tccggggtgg ttcgcacggt gctcggcgac gtcgatccgg
     1201 cggcgctggg tcgtgtgaac taccacgagc acctgttcca gatctcgccg ctgctgcccg
     1261 gcgacgagct cgacgacgaa cgtgcctcag gtctcgaagc ggcgtcgctg aaggcgagcg
     1321 gcttcgagac gatggtggat gccacgccct tcgggctcgg tcgcgatcct gaagcgctcg
     1381 cacggatcag cgcagccacc ggcctcgccg tggtcgcgac gagcggccgg caccgggagg
     1441 cgcactacgc ggccgaccat ccgacgcggt cgtgggacgc ctcgcgattg gcggccctgt
     1501 tcgcggccga tgttcaggcc gggatgccgg catccgacga tgagcccgta cggaacggat
     1561 gcacgactgg cgaacagttg cacgaccggg acgccgattc cgtcgtgcat ccgttcccgg
     1621 atcgtgcagg tgtacccgcg gtcggtcctt ccggagagcc agtgcgcgcc ggcatgctga
     1681 agggcggcgt cgactactgg cggatcagcg cgttcgagcg catcaccctg gatgccctgg
     1741 gcgaagcgca ccgcagcacc ggcgccccgg tcatggtgca cctcgagttc tgcacggccg
     1801 cccacgaggt gctcgacctg ctcgcggccg agggggtggc gtccgaccgt gtcgtgctgg
     1861 ctcacgccga tcgcgatacg gatgccgggc tgcacctctc cctgaccgag cgcggcgcct
     1921 tcctcggcta cgacggcttc gcccgcccgc gcacgcgctc agacgcagag ctgctgcagc
     1981 tcaccgcatc cgtcgtcgcg ggtggaggtg ccggtcgcat cgtgctgggc ggcgacgtcg
     2041 cccgcgcgac ccgctacatc gcctacggcg gcatgcccgg cctcgcctac ctcggcgagc
     2101 ggtacgttcc gcgtctgcgc gccctggtcg gcgacgaggc ggtcgaccgc atgctggtcg
     2161 acaatcccgc ccgactgctg acgctgtccg caccgtcctc ctgaaggagc accatgtccg
     2221 agcccacgat cctgcacgcg accttcacgg cccgccccgg cgagggcgat cgcgtcgccg
     2281 cactgctgcg cgacttcgcg gaggctgtcc gcgccgagcc cggcaatgtc gtcttcgacg
     2341 ccacccgcgt gctcgacgcc ccggacagct tcttcgtcta cgaggagtac gtcgacgagg
     2401 cggcgttccg ggcgcacctg gcgaatcccg ccggcggccc gttcaacgcg gcgctgcagg
     2461 agctgatcct cgagccgtcc tccgtgctga ccttcctgcg gcgcatctga ggcggacgcc
     2521 gcacgccgac cgcacgcgtg ccagccccac ggccctacgt cgcccgcctc cgggtcgcag
     2581 ttggtcctgg tatccggtgc ccccgacact ccaggtcgca gttggtctcg gcatccgagc
     2641 accagagcgg gacgaagtgc gacctggacg gcatccgagc gccgagaacg ggatgaactg
     2701 cgatctgaag gtccctgccg gcttcgctcc ctcgtccggc aggccagcga gaagggcccc
     2761 tcacccgaag gcgaggggcc cttctgcgga tcagacgatc agcgggtcac tggtagagga
     2821 ccgcggagat cttcgggtcg tccaggttgg tggcgtcgta ccagtacgag ccggtgtcgt
     2881 agaacttctc gacggacttg ccgttcgcga ggtcgtaggc ggcctgcacg acggtcttgc
     2941 cgatgccgat cgggtcctgc gtgatcgcgc cggcgaggac gccgcccttg atcgcgtcga
     3001 tctgggccgc gcccgagtcg aagccgatcg cggtgatgtc cttgctggtc ttgccgagct
     3061 cgctcagggc gttcgcgacg ccgacggcgg aaccctcgtt ggtcgcgtag atgcccttga
     3121 ggtcggggtg cgcgttcagc atcgacttgg tgatgtcggc cgacttcagg tggtcgccgt
     3181 caccgtactg gatgtcgacg atcttgatgt cggggtagtc cgccttgatc ttgtcgacga
     3241 agccgtcgcg gcgctcgaca ccggtcgagt tgatctgcga gtgcgcgacg atcgcgacgt
     3301 cgcccttgcc accgatgagc tcggccatgt gctcggccgc gagcgcgccg gcgaccttgc
     3361 tgtcggtcgc ggcgagggtc ttgccgacct cgctgttgca cggggcgtcg aagtacgcga
     3421 ccgggatgtt cgcagaggca gcggcgtcca gagcggggat gcaggcctcg gggtcgaggg
     3481 ccgcgtacgc gatggcggac ggcttgcggt cgatcgacga ctgcagcatc tggagctgct
     3541 tctcgatctc ggtctcggag ccggggccct cgaagttgat cttgacgccg agctccttgc
     3601 ccttctcctc ggcacccttc ttcacggcct gccagaactg gtgctggaag cccttcgaga
     3661 ccaggtcgat cgtcatgtcc ttggcagcgg gcttgtcgcc gccgccggcc ggggtgtcac
     3721 ccttgccggt ggcgcagccg gccaggctca gcaccgtcag cgctgcgacg gtcgcgacgg
     3781 cgatcttctt tccgtacttc atgtgaaact ccatcgttct gtgattgtgg acgttctcgg
     3841 gtggacgtgc ggtgttcagt tgggaaggga ggtcgttcag ctcttgccgc ggttgcgcag
     3901 cgcgtcgaag aacacggcga tgaggatgac gatgccggtg aggatgtact gccactcggt
     3961 ctggatcgac atgctgcgca gaccgttgat cagcacgctc atgatgaagg cgccgatcac
     4021 ggtgccgacg atcgatcctc ggccgcccat cagagaggtg ccgccgatga caacggccgc
     4081 gatggcctgg agctcgtaac ccgcgccggc ggtcggctgc gcggagccca ggcgggcggt
     4141 cagcagcaca ccggcgatgc cgatgaacag gccggcgaag acgtagaccc agatggtcca
     4201 gcggcgcgtg ttcacaccgg agaggcgggt ggcctcctcg ttggagccga tcgcgaacgt
     4261 gtagcggccc agcagggtct tgttcagcgt caggtatgcg atgacggcca gcacgacgaa
     4321 gatgagcacg cccatcggga ggctcgcgaa gtagccgtcg gacgccgtga gcacggggtc
     4381 gaactggacg atgctcgtga cctgcttgcg cgtctcagga gcgaaggtga tcggcttcac
     4441 ctccgagatg accagcgcca gaccctgcgc gatcatcatc atcgcgagcg tggcgatgaa
     4501 gggaggcaga cgcaggaagg tgacgttgag cccgttgatc aggccgatca gcgcaccggt
     4561 ggccagcgcg ccgagcacgc ccacccacac gggcagaccc catccggtga tgaacgttcc
     4621 catcatcacc gagcacagcg tcatgccgaa gccgagcgag aggtcgatgc ccgcggtcac
     4681 gatcacgaag gtcgtgccca gtgcgagcac gccgacgagc accgtcgaca tgaggatgtt
     4741 ctcgatgttg gaggaggacg tgaagttcga tccgaagatc gcgaagaaca cgaacagcac
     4801 gatcagcgtc ccgagggcga gcccctgctg aaccgagttg cgcagagccg cgccgaggcg
     4861 gcccttctgg gtgttctcgg acaccgcggt ctgcacgacc gtcgtggtcg agttgctcat
     4921 cagctgtcct ctccgtgggc tgcgagatgc atgatcttct cctgggtggc ttcctcgttg
     4981 cggagctcac cggtgacctc tccgttcgcg aacacggtga tgcggttcgc gacgcggagc
     5041 agctcgggga gctcggacga gatgacgatg atggacttgc ccgagtcggc gagctgctgc
     5101 atcaggcggt agatctcctc cttggctccg atgtcgatgc cgcgggtggg ctcgtcgaag
     5161 atgaggatgt cgcagtcgcg catcagccag cgggcgatga cgaccttctg ctggttgccg
     5221 cccgagagca gcttgacgat ctggttgacc gagggcgtct tcacgcgcag ctgctgcacg
     5281 tagtccttgg tcttctgctt ggccgtgcgg tcgcccatcc agccgagcgc accggcgtag
     5341 gagttgagcg aggcgagcac cgtgttgaag gtgacgtcct gctccagcat cagacccagc
     5401 agcttgcggt cctcggacag gtatccgatg ccctgcttga cggcgtcctc gggctggcgg
     5461 atgcgccgct ccctgccgcc gatcacgatg ctcccgccgt cgcgcggatc cgctccgatg
     5521 acggcacgag cggtctcggt gcgtccggcg cccatcagcc cggcgaagcc gaggatctca
     5581 ccctggtgca ggcggaagga cacgtcgcgc agcagcgact tggtggacag gccctgcacc
     5641 tcgaggacca ccggctcggt ggtgtagtcg cgcggcgcgg ggcgcgctcc ctcgtcgatc
     5701 gcgcgtccca ccatcatctc gatgatcttg gggatgctga tctcgtcctt ctcgagagaa
     5761 ccgatgtatg cgccgtcgcg cagcacgctg acccggtcgg cgaggcgccg cagctcgtcc
     5821 atgcggtgcg agatgtagac gatgccggtg cccttggcct tgagctgctc gatgagggtg
     5881 aacagcacct cgacctcggt gtcggtgagg gccgatgtcg gctcgtccat gatcagcacc
     5941 ttggcgttga acgagagcgc cttggcgatc tcgaccatct gctgctccgc gacggtgagc
     6001 tcgcccacct tctgacgggg gtcgaggtgg atgtcgaggc gctgcagcag ctccgccgtc
     6061 tgctggttca ggcgccgctc cgagacgaag ggtccgacct tcggctcgcg gccgatgtag
     6121 atgttctgcg cgaccgtgag gtcgggcatc atgttcagct cctggtggat gatcgtcaca
     6181 ccgagctgct gggcctgcag cggactggtg aactcgaccg gctcaccctc gaaggtgatg
     6241 gtcccctcgt cacgcgtgta gatgcccgag aggatcttca tcagcgtcga tttgcctgcg
     6301 ccgttctcgc cgacgaggac caggacctct cccgaccgca cttcgaggtg cacgtccttc
     6361 agcgcctgca cgccggggaa ccccttgctg attccctcca ccttgaggat gggatcgctc
     6421 atgcccgcct actccctcgg aggtcgagga ttcccgtgga atccattcca aacatcggat
     6481 caattgtgcg cgtctgcgtc ctgatgcgca agttgtatct aaatgtatct aacctcgccg
     6541 cacagcgcca gacctgtaat aacaagttgg tcacggtagc ctcgccgccc agcacagccg
     6601 gatgactcag tgcagccgga tggaatcgac ggcgacgagc ttgtcccgga tgaatcggtt
     6661 cgcgtgcgcg ccgagctcct cgctgtcggt ccacaggttg cgccagatcc ccagcatccg
     6721 gctcaggtcc tccgcgacca ccgccgacga gaacgactcg aagacgatgg ggccgtcata
     6781 gccgatccgg ccgagcgctt tgaagaaggt gtcgaagtcg accgtcccgg tgccgaggta
     6841 gccgcggtgg ctctcgccga tgtgcacgta gcggagaccg ggtgcggcat ccagcacggg
     6901 agccgccatg tccgactcct cgatgttcat gtgataggtg tcgagatgga tgcccagatt
     6961 cggccggtcc acctccgcga ggtagccgat cgcctgccgt gcggtgttca ggacgttggt
     7021 ctcgtagcga ttgacgacct cgaggctgac ctgcacgccc cgggaggcgg cgtggtccgc
     7081 gatgcgtccg atgacggccc tgctgctcgc gaggccctcg ggcgtgaccg gcatcatgta
     7141 cttgcgcatc gccgagtaga tcacgccgca gaagtgcgtg ccgccgagct cagccaggac
     7201 gtcgacagcg cggatcagca gcgcctcgcc cgccgccacg acgtcggcgt ccgcgctggt
     7261 gatgtcggtc gcatccgaga gccccaggga cgcactcacc gcaagaccat gctcggcgag
     7321 cgcctccttc gcggcctcga catcgaagga gaacgggtcc atcagcggga actcgatgag
     7381 gtcgaacccg gccgctgcgg tcttctccac cgccaggcgg atgcctgccg cgtcgaattc
     7441 cccggtccag accaggccat ggcatccgat gttcataaga gctcctttgc tgaattggca
     7501 cgttgcaatt ccacgttgag gcgacggtac tctgggagct tgcaacgtgt caatacccgg
     7561 gtatggtgac tttcaccacc cgattcgagc ggagaggacg ccggatgccg gccagtgtca
     7621 aggacgtcgc cgcccgcgcg ggtgtctcgg cctccaccgt gtcgaactac ctcaaccatc
     7681 cgcacgtgct cagcgagagg accagggagc gcgtgcggaa cgccgtcgcc gaactcggct
     7741 tcgtgccgaa cgagtcggcc cgccagctgc gcgccggagc gggcaggacg atcgcgctga
     7801 tcctgctcga tgcctggctg ccgtacttca gcgagctctc ccggggtgtc gaggacgtca
     7861 cccgggaggc cggctggtcg ctgttcttca gcaacagcaa ccgcgatgcc gcacaggagc
     7921 agcgcaacat cgacatgttc gaggcgcatc gcgcgcaggg catcctcatc tacccgctcg
     7981 gcgacgtgct cggccggctc gagggcctgg cggacaaggg catccgctcg gtcgtcatcg
     8041 gccccgagca gccgtcgtcc tccgtcggct ccgtggcgtt cgacgaccgc ggcggcggcc
     8101 ggctcgccgg agagcacctg atcgggatcg gccgtcgccg catcgccttc ctcggcgatc
     8161 cccgcacggt cagtcagtcc gcggatcgcc tcgcgggcct ccgggacgcg gcgccggatg
     8221 cccggatcga cgtgatcgcg acggacggac tcacgatcga tgcggggctg ctggcggcgg
     8281 agcggatcat cgccgcgccc gcatcggagc gcccggacgc cgtgttcgcg gcaaacgaca
     8341 tgctcgcgat cggcgcccag accgcactgc tgcgcgcggg gatcgcggtt cccggcgaca
     8401 tcgcgatcgt cggcttcgac gacgtcgatc aggcccgcca ggcactggtc cccctcacca
     8461 ccatccggca gccgggctac gagatcgggc gcgccgcggc gtcggcactg ctccgcctgc
     8521 tggaggctcc gagcacaccc gccgcgccgg tcacgccct
//
LOCUS       DATMSX010000115         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_124926,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000115 DATMSX010000000
VERSION     DATMSX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124926"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VN041_03635"
     CDS             <1..884
                     /locus_tag="VN041_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWU28170.1"
                     /translation="WTALLDPTGALRPGIPLCPPEGDAGTGMVATNAVAPRTGNVSAG
                     TSIFAMVVLEHPLAEVHHELDLVTTPAGDAVAMVHCNNGASELAAWVGMFTRFAEASG
                     IRGSSDAVFETLFREALDGEADAGGLLAYNHLAGEPIAGLDAGRPLFVRTPDSRFTLA
                     NVMRAQLYGVFGTLALGMQVLTDEGVALDRMYAHGGMFRTAGVAQRFLAGALNAPVAV
                     GDTASEGGAWGIAVLAAYLSAAGEQPLDAYLDQHVFAGASLSVIDPDPADVAGFTTYL
                     DRYRAGLAIEAAATGSI"
     gene            896..2413
                     /gene="araA"
                     /locus_tag="VN041_03640"
     CDS             896..2413
                     /gene="araA"
                     /locus_tag="VN041_03640"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HWU28171.1"
                     /translation="MPKLTTSLDGYQVWFLTGSQHLYGPETLAQVADQSRAISDALDG
                     ASEVPVRIVWKPVLTDSAAIKKVMLEANADDSVIGVVAWMHTFSPAKMWIAGLDALQK
                     PLAHLHTQANVELPWADIDFDFMNLNQAAHGDREFGYIQTRLNVPRKTIVGHASDPRV
                     RHELGTWQRAAAGLAASRSLKLARFGDNMRFVAVTEGDKTEAELRLGVQVNTWGVNEL
                     ADAVAAASESDIDALVAEYEELYEVVPELRRGGERHQSLRDGAAIEIGLRSFLEEGGF
                     GAFTTSFEDLGALKQLPGLAVQRLMAEGYGFGAEGDWKTAILVRVANVMGSGLPGGAS
                     LMEDYTYDMTPGDELILGAHMLEVSPSLTSAKPTLEIHPLGIGGKDDPVRLVFTADPG
                     PAVVVALSDMRDRFRLTANVVENVEPRAALPKLPVGRAVWKPAPDFNTSAAAWLTAGA
                     AHHTVMSTAVGLEAFRDFAEMAQIELLVIDGSTTLPEFQKTVRWNQAYYRLAQGF"
BASE COUNT          365 a          905 c          842 g          369 t
ORIGIN      
        1 aatggactgc gctgctcgac ccgaccggag cactgcgccc tggcatcccg ctctgcccgc
       61 cggagggcga cgcgggtacg ggcatggtcg cgacgaacgc ggtcgcaccg cgcaccggca
      121 atgtgagcgc gggcaccagc atcttcgcga tggtcgtgct cgagcatccg ctggccgagg
      181 tgcaccacga actcgacctg gtcaccactc ccgccggtga cgcggtcgcc atggtgcact
      241 gcaacaacgg cgccagcgag ctggccgcgt gggtgggcat gttcacccgg ttcgccgagg
      301 cgtccggcat ccgcggatcg tcggatgccg tcttcgagac gctcttccgc gaggcgctgg
      361 acggcgaggc ggatgccggc ggactgctcg cctacaacca cctcgccggc gagcccatcg
      421 ccggcctcga cgcggggcgt ccgctgttcg tgcgcacgcc cgacagtcgc ttcaccctgg
      481 cgaacgtcat gcgcgcccag ctctacggcg tgttcggcac gctcgcactg ggcatgcagg
      541 tgctgaccga cgagggcgtc gcgctcgacc ggatgtatgc ccacggcggc atgttccgca
      601 ccgccggtgt cgcgcagcgc ttcctggcgg gggcgctgaa cgcacccgtg gcggtcggcg
      661 acacggcatc cgagggcgga gcgtggggca tcgcggtgct cgccgcctac ctgtcggcgg
      721 ccggcgagca gccgctggat gcctacctgg accagcacgt cttcgcgggg gcatccctct
      781 ccgtcatcga ccccgacccg gccgacgtcg ccggattcac cacctacctc gaccgttacc
      841 gcgcgggtct ggccatcgag gccgccgcca caggctccat ctaaggattg cgtccatgcc
      901 caagctcacc acctccctcg acggctacca ggtctggttc ctcaccggca gccagcacct
      961 gtacggaccc gagacgctcg cgcaggtcgc agatcagtcg cgtgccatct cggatgcgct
     1021 cgacggcgcc tccgaggtgc ccgtccgcat cgtgtggaag cccgtgctga ccgactccgc
     1081 cgccatcaag aaggtcatgc tcgaggcgaa cgccgacgac agcgtgatcg gcgtcgtcgc
     1141 gtggatgcac accttcagcc ccgccaagat gtggatcgcc ggcctcgacg ccctgcagaa
     1201 gccgctcgcc cacctgcaca cccaggcgaa cgtcgagctg ccctgggccg acatcgactt
     1261 cgacttcatg aacctgaacc aggccgcgca cggcgaccgc gagttcggct acatccagac
     1321 ccggctgaac gtgccgcgca agaccatcgt cgggcatgcg tccgacccgc gcgtgcgcca
     1381 tgagctcggc acctggcagc gggccgcggc cggcctcgcg gcctcgcgga gcctcaagct
     1441 cgcgcgcttc ggcgacaaca tgcgcttcgt tgcggtcacc gagggcgaca agaccgaggc
     1501 cgagctgcgc ctgggcgtgc aggtgaacac ctggggcgtg aacgagctgg cggatgccgt
     1561 cgcggcggca tccgaatccg acatcgacgc tctggtggcc gagtacgagg agctgtacga
     1621 ggtcgtcccc gagctgcggc gcgggggaga gcggcaccag tcgctgcgcg acggcgcggc
     1681 catcgagatc gggctgcggt cgttcctgga ggagggcggc ttcggcgcct tcaccacctc
     1741 gttcgaggac ctgggcgcgc tgaagcagct gccgggcctg gccgtgcagc ggctgatggc
     1801 cgagggctac ggcttcggcg ccgagggcga ctggaagacc gcgatcctgg tgcgcgtcgc
     1861 caacgtgatg ggctccggcc ttcccggcgg cgccagcctg atggaggact acacctacga
     1921 catgaccccg ggcgatgagc tgatcctggg agcgcacatg ctcgaggtct cgccctcgct
     1981 cacctcggcc aagccgaccc tggagatcca tccgctcggc atcggaggca aggacgaccc
     2041 ggtgcgcctg gtcttcaccg ccgaccccgg tcccgctgtc gtcgtggcgc tcagcgacat
     2101 gcgcgaccgc ttccgcctga ccgcgaacgt ggtcgagaac gtcgagccgc gtgcggcgct
     2161 gccgaagctc cccgtgggtc gtgccgtgtg gaagccggct ccggacttca acaccagcgc
     2221 tgctgcctgg ctcaccgccg gtgccgcgca ccacaccgtg atgtcgaccg ccgtcggcct
     2281 ggaggcgttc cgcgacttcg ccgagatggc gcagatcgag cttctggtga tcgacgggtc
     2341 gaccactctg cccgagttcc agaagacggt gcgctggaac caggcgtact accggctcgc
     2401 gcaggggttc tgacccagcg ccctgcactc cggagctctg cgctccggag ccctgcgctc
     2461 cggagccctg cgctccggag c
//
LOCUS       DATMSX010000116         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_77273,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000116 DATMSX010000000
VERSION     DATMSX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77273"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VN041_03645"
     CDS             <1..996
                     /locus_tag="VN041_03645"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HWU28172.1"
                     /translation="GAAARLGAIVRATDPRAEVADQVTSVGGVYLPVDVAVEKSTDGY
                     AKATSEDYNRRAAEIYTEQAADVDIIITTALIPGRAAPRLITASDVALMKPGSVIVDM
                     AAGQGGNVEGSVAGERTVTDNGVIILGYTDLASRLSAQASQLYGTNVANLVALLTPNK
                     DGALTFDFDDVVQRSVTVVRDGEVTWPPPAVQVAAAPASAPVEAAAEVPAKQPLSVGK
                     KVGLIVAGIAALFLVNVFAPAPLPQHFTVLMLSVVIGFYVIGKVHHALHTPLMSVTNA
                     ISGVIIVGAMLQITAANPVVQVIAAVAVLVASINIFGGFAVTRRMLSMFVKGGSK"
     gene            993..2393
                     /gene="pntB"
                     /locus_tag="VN041_03650"
     CDS             993..2393
                     /gene="pntB"
                     /locus_tag="VN041_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HWU28173.1"
                     /translation="MTVDAIAGGAYIVAALLFILSLAGLSRHETARAGVIYGIVGMAI
                     ALLSTLALTVADAWGGAGSALGLTLMAAGVLVGAAIGLWRAEVVQMTQMPELIAMLHS
                     FVGIAAVLVGWNGYLAHEPIAENLVGIHNAEVFIGIFIGGVTFTGSIVAYLKLSAKIS
                     SRPLMLPGKNALNIGALVLFLVLTVWFVMDQQLWLLITVTLLSFALGWHLVASIGGGD
                     MPVVVSMLNSYSGWAAAAAGFLLNNDLLIVTGALVGSSGAYLSYIMCKAMNRSFISVI
                     AGGFGIVASASSDEDFGEYRELTAESAAAMLESASSVIITPGYGMAVAQAQYPVADLV
                     ARLRERGVDVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLGMDEINDDFASTSVVLVI
                     GANDTVNPAAAEDPGSPIAGMPVLKVWEAENVIVFKRSMAAGYAGVQNPLFFRENAQM
                     LFGDAKERVEDIIHAL"
     gene            complement(2455..2961)
                     /locus_tag="VN041_03655"
     CDS             complement(2455..2961)
                     /locus_tag="VN041_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28174.1"
                     /translation="MSAEAQPEIIVRPVRDADAEALGRVHAQTWHETYDHLISKAALE
                     RISPKRLAELWVNWARQGEDFKMSAALVDGEIVGFVGSGPARDKDAPSLRELYFIYLL
                     DAWHGTGIGQKLFDAAVDKDESLYLWVAEDNPRAHRFYERNGFTLDGAAHTEPFLGET
                     LTEVRFVR"
     gene            3081..3641
                     /locus_tag="VN041_03660"
     CDS             3081..3641
                     /locus_tag="VN041_03660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWU28175.1"
                     /translation="MNVIWRTLLVILQARSRVRRGRTLDSAEVGRIRLTTLPTDIDIL
                     RHMNNGRYLSLFDLGRWDLLIRTGLFDAMKERGWYAVVSSETVTFRKSLQLWQRFDVE
                     SRFIGHDEKAVFMEHRAVVDGEVYARVIVRARMMRRSGGTVGHDELFAAVGTPAGVPD
                     IDDWIHDWATASALPPTRKPAPSVWR"
BASE COUNT          585 a         1339 c         1305 g          624 t
ORIGIN      
        1 ggcgcggcag cccgcctggg cgccatcgtg cgggccacgg acccgcgggc cgaggtcgcc
       61 gatcaggtca cgtcggtcgg cggcgtctac ctgcccgtcg acgtcgccgt ggagaagtcc
      121 accgacggct acgccaaggc caccagcgag gactacaacc gccgcgctgc cgagatctac
      181 accgagcagg ccgcggacgt cgacatcatc atcaccaccg ccctcatccc ggggcgcgcg
      241 gcgccgcgcc tgatcacggc atccgatgtc gccctgatga agcccggcag cgtgatcgtc
      301 gacatggccg caggccaggg cggcaacgtc gagggctccg tcgcggggga gaggacggtc
      361 accgacaacg gcgtgatcat cctgggctac accgacctcg cctcacggtt gtccgcgcag
      421 gcatcccagc tgtacggcac aaacgtcgcg aacctggtcg ccctcctcac accgaacaag
      481 gacggggcgc tcaccttcga cttcgacgac gtcgtgcagc gctcggtcac ggtcgtgcgc
      541 gacggcgagg tcacctggcc gccgcccgcc gtgcaggtcg ctgcggcacc ggcatccgct
      601 cccgtcgagg cggccgcgga ggtccctgcc aagcagccgc tgtcggtggg gaagaaggtg
      661 ggcctgatcg tcgccggcat cgcagcactg ttcctcgtga acgtcttcgc gcccgccccg
      721 ctgccgcagc acttcacggt gctcatgctc tcggtcgtga tcggcttcta cgtgatcggc
      781 aaggtgcacc acgcgctgca caccccgctc atgtcggtca ccaacgccat ctcgggcgtg
      841 atcatcgtcg gcgccatgct gcagatcacg gccgcgaatc ccgtcgtgca ggtgatcgcg
      901 gccgtcgccg tgctggtcgc cagcatcaac atcttcggcg gcttcgccgt cacccgccga
      961 atgctcagca tgttcgtgaa gggcggaagc aagtgaccgt cgacgccatc gccggcggcg
     1021 cctacatcgt cgccgccctg ctgttcatcc tgagcctcgc ggggctcagc cgccacgaga
     1081 ccgcccgcgc gggcgtgatc tacggcatcg tcggcatggc catcgccctg ctgtcgacac
     1141 tcgccctcac ggtcgcggat gcctggggcg gggcgggctc cgcgctcgga ctcacactga
     1201 tggccgcggg cgtgctcgtc ggcgccgcca tcgggctgtg gcgcgccgag gtcgtgcaga
     1261 tgacccagat gcccgagctc atcgccatgc tgcacagctt cgtgggaatc gcggccgtgc
     1321 tggtcggctg gaacgggtat ctggcgcacg agcccatcgc ggagaacctg gtcggcatcc
     1381 acaacgccga ggtgttcatc ggcatcttca tcggcggcgt gaccttcacc gggtcgatcg
     1441 tcgcctatct gaagctctcc gcgaagatct cgtcgcgccc gctcatgctg ccgggcaaga
     1501 acgccctgaa catcggcgcc ctggtgctgt tcctcgtgct cacggtctgg ttcgtgatgg
     1561 accagcagct ctggctgctg atcaccgtca ccctgctctc gttcgcgctc ggctggcacc
     1621 tggtggcctc gatcggcggc ggcgacatgc ccgtggtcgt ctcgatgctg aacagctact
     1681 ccgggtgggc ggcggccgcg gccggcttcc tcctgaacaa cgacctgctg atcgtgaccg
     1741 gtgcgctggt gggctcctcg ggtgcgtacc tgtcgtacat catgtgcaag gccatgaacc
     1801 ggtcgttcat ctcggtgatc gcgggcgggt tcgggatcgt ggcatctgcc tcgagcgacg
     1861 aggacttcgg cgagtaccgc gagctcaccg ccgagtccgc cgccgcgatg ctcgagagcg
     1921 cgtccagcgt gatcatcacc ccgggctacg gcatggccgt ggcgcaggcg cagtacccgg
     1981 tcgccgacct ggtcgcccgg ctgcgcgagc gcggcgtgga cgtgcgtttc ggcatccacc
     2041 cggtcgccgg gcgcctgccc ggccacatga acgtgctgct ggccgaggcg aaggtgccgt
     2101 acgacatcgt gctgggcatg gacgagatca acgacgactt cgcctcgacg agcgtcgtcc
     2161 tggtgatcgg cgcgaacgac accgtgaacc cggcggcggc cgaggacccg ggcagcccga
     2221 tcgcggggat gcccgtgctc aaggtgtggg aggccgagaa cgtgatcgtg ttcaagcgct
     2281 cgatggccgc ggggtacgcg ggcgtgcaga acccgctgtt cttccgcgag aacgcgcaga
     2341 tgctgttcgg cgacgcgaag gagcgggtgg aggacatcat ccacgccctg tgagcagcgg
     2401 aacaccagtg cggccctccc ggatatccgg agggccgcac tcatgtagtc acgctcagcg
     2461 cacgaagcgc acctcggtga gggtctcgcc caggaacggc tcggtgtgag cagcgccgtc
     2521 gagggtgaag ccgttgcgct cgtagaagcg gtgcgcacga gggttgtcct cggcgaccca
     2581 caggtacagg gactcgtcct tgtcgacggc ggcgtcgaac agcttctggc cgatgccggt
     2641 gccgtgccac gcgtcgagca ggtagatgaa gtacagctcg cgcagcgacg gtgcatcctt
     2701 gtcgcgggcg ggaccggagc ccacgaagcc gacgatctcg ccgtcgacga gcgcggcgct
     2761 catcttgaag tcctcgccct ggcgggccca gttcacccac agctccgcga gccgcttggg
     2821 ggagatccgc tcgagggcgg ccttgctgat caggtggtcg taggtctcgt gccacgtctg
     2881 cgcgtgcacg cgacccagag cctccgcatc cgcatcgcgg accggacgga cgatgatctc
     2941 gggctgggct tcggcgctca tgccgcgact ctacgcgcgc tttcgcggca ggtgaaatcg
     3001 agtggaccgt gcatgacgcg tatgtcgtca tccgacaacc ggattgcggc cgtggttgcc
     3061 gatagctact gtcgatgctc gtgaatgtga tctggcgcac cctcctcgtc atcctgcagg
     3121 cgcggagccg cgttcgtcgg ggacgcacgc tggattccgc cgaggtcggc cgcatccgac
     3181 tcaccaccct ccctacggac atcgacatcc tgcggcacat gaacaacggc cggtacctgt
     3241 cgctgttcga cctggggcgc tgggacctgc tcatccgcac cgggctcttc gacgccatga
     3301 aggagcgcgg ctggtacgcc gtggtctcca gcgagacggt caccttccgc aagtcgctgc
     3361 agctgtggca gcggttcgat gtcgagtccc gcttcatcgg ccacgacgag aaggcggtgt
     3421 tcatggagca ccgcgcggtc gtcgacggcg aggtgtacgc gcgggtcatc gtgcgggctc
     3481 ggatgatgcg ccgctcgggc ggcacggtgg ggcatgacga gctgttcgcc gcggtcggca
     3541 cccccgcagg agtgcccgac atcgacgact ggatccacga ctgggcaacg gcatccgctc
     3601 tgcccccgac gaggaagccg gcgcccagcg tctggagatg agcctcacgc ctccggagga
     3661 cggaccgtcc cctctaagct gagaggcgtc atcgaggggg aatcatgtcg tacgatccga
     3721 acgcgggcga gcccgcgcag ccgcagcagc cgcctgtccc gccgcagtac cagcctccgg
     3781 gcgcggttcc gccggtgaac cagccgacgg ctcctcagca gccgacgtac cagccccagc
     3841 ccggccagcc cca
//
LOCUS       DATMSX010000117         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_77308,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000117 DATMSX010000000
VERSION     DATMSX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77308"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1052
                     /locus_tag="VN041_03665"
     CDS             210..1052
                     /locus_tag="VN041_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HWU28176.1"
                     /translation="MTKTTWATALQVGEGFRLEDVDAGSTPGFDSGKKQGAKELAARR
                     EVIGELQERLFAESRAGIAKDAVLLVLQAMDTAGKGGIVRHVVGGVDPQGIELVAFKA
                     PTDEERAHDFLWRVESRLPQPGFIGVFDRSHYEDVLIGRVRKLASEEEIERRYGAIAD
                     FERRVAASGIRIVKVMLHISPDEQKARLMERLDRPDKHWKYNPGDVDERLRWGDYMAA
                     YQTAFERTSSAEAPWHVIPANHKWYARLAVQELLIAALEDIDPQWPAADFDVEAEKKR
                     LEAS"
     gene            complement(1062..1775)
                     /locus_tag="VN041_03670"
     CDS             complement(1062..1775)
                     /locus_tag="VN041_03670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU28177.1"
                     /translation="MEPNRADLGKDPSHVSGMFDQVAAGYDRTNTVMTFGNDAFWRAA
                     TTRAVAPKRGERILDLAAGTASSSASLAGSGAQVVAADFSPGMLAEGRRRHGHLPNIT
                     FVEADATNLPFKDGEFDAVTMSYGLRNVQEPKKALAELFRVTKPGGRIVINEFSTPPG
                     KLFRGLYRFYNAQVLPKVARLAGTNGDAYDYLNESIRDWPDQRTLAGWIREAGWTDVE
                     YRNLSFGIVALHRGRKPLA"
     gene            1952..2557
                     /locus_tag="VN041_03675"
     CDS             1952..2557
                     /locus_tag="VN041_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28178.1"
                     /translation="MSANRPEPGTPMIVQWRKWDGSPHWTNEQVYLGSDRWGDWVGQP
                     RGWRSSRPGREFTASAPNITLIPHPDPDRGAADLALTINRGHPKGMRIYIDLAWDLRW
                     TADAEDGAPGMPALAHGIDMDLDVVRVEGERGTWVDDRDEWAEHLVQYGYPADVIAHL
                     EALALDLEQKVRAQVAPFDDATADPWLDRLEALGMERRPEK"
BASE COUNT          433 a          924 c          996 g          376 t
ORIGIN      
        1 gctcgccgcg gcctgactcg cggcatccgt ggcacgcccg cgctgtgtgc cgagcacgaa
       61 gccgagcccg ccggcaaggg cggcggcgat cacagcgacg acgatcacga gaagcgaggt
      121 gtccatgatc cgattgtgca cggggccacc gacacttcgt ctcgcggcac tgcggcgcac
      181 ccggccgcgc acccggcaca atggcaggca tgacgaagac gacatgggcc acggcactgc
      241 aggtcggcga gggcttccgg ctggaggatg tcgacgccgg cagcactcct ggcttcgaca
      301 gcggcaagaa gcagggcgcg aaggaactcg ccgcccgccg cgaggtgatc ggcgagctgc
      361 aggagcgcct gttcgcggaa tcgcgcgccg gcatcgccaa ggatgccgtg ctgctcgtgc
      421 tgcaggcgat ggacaccgca ggaaagggcg gcatcgtgcg gcacgtggtc ggcggagtcg
      481 acccgcaggg catcgaactg gtggcattca aggcaccgac cgacgaggag cgcgcgcatg
      541 acttcctgtg gcgggtcgag agccgcctgc cgcagcccgg cttcatcggc gtcttcgatc
      601 gctcacacta cgaggacgtg ctgatcggcc gggtgcgcaa gctggcatcc gaggaggaga
      661 tcgagcgccg gtacggcgcc atcgccgact tcgagcgccg cgtcgcggca tccggcatcc
      721 gcatcgtcaa ggtcatgctg cacatctcgc cggacgagca gaaggcgcgt ctgatggagc
      781 gcctggaccg cccggacaag cactggaagt acaaccccgg cgacgtcgac gagcgtctgc
      841 gctggggcga ctacatggcg gcgtatcaga ccgcgttcga gcgcacctcg tcagccgagg
      901 cgccctggca tgtgatcccg gcgaaccaca agtggtacgc gcgcctcgcc gtgcaggaac
      961 tgctgatcgc cgccctcgag gacatcgacc cgcagtggcc cgcagccgac ttcgacgtgg
     1021 aggccgagaa gaagcgcctc gaggccagct gagcgctgag ctcaggccag cggcttgcgt
     1081 ccgcggtgca gggcgacgat cccgaacgac aggttgcggt actcgacgtc cgtccagccg
     1141 gcctcgcgga tccagcccgc cagcgtgcgc tggtccggcc agtcgcggat cgactcgttg
     1201 aggtagtcgt aggcatcgcc gttggtgccg gcgaggcggg cgaccttcgg cagcacctgc
     1261 gcgttgtaga agcggtacag cccgcggaac agcttgcccg gaggcgtgga gaactcgttg
     1321 atcacgatgc gcccacccgg cttcgtcacc cggaacagct cggccagagc cttcttcggc
     1381 tcctgcacgt tgcgcaggcc gtacgacatc gtgacggcgt cgaactcgcc gtccttgaag
     1441 ggcaggttcg tggcatccgc ctcgacgaac gtgatgttgg gcaggtgacc gtgccggcgg
     1501 cggccctcgg ccagcatgcc gggggagaag tcggcggcga cgacctgcgc gccgctgccg
     1561 gcgagggagg ccgaggagga tgcggtaccc gcggcgaggt cgaggatccg ctcgccgcgc
     1621 ttcggggcga cggcgcgcgt ggtcgccgcg cgccagaagg cgtcgttgcc gaacgtcatc
     1681 acggtgttcg tgcggtcgta gccggcggcg acctggtcga acatgccgct gacgtgcgag
     1741 gggtctttgc cgaggtcggc gcggttcggc tccatgcttc gatcctaaag cgggcgggtg
     1801 ccgtcgcgcg cccgcacgga tgtcggtcga aagcacggat gtcggccgga tccgagcatc
     1861 cgagccgaca tccgtgttct gagccgacat ctgtgcgagg ggcgtgtcgg cccccgatct
     1921 gtgtcggttc ggccaggcat agactcggcg ggtgagcgcg aaccgtcctg agcctggcac
     1981 cccgatgatc gtccagtggc gcaagtggga cggctcgccg cactggacca acgagcaggt
     2041 ctacctgggc tcggatcggt ggggcgactg ggtcgggcag ccgcggggat ggcgcagcag
     2101 ccgtcccggg cgcgagttca cggcatccgc tccgaacatc acgctcatcc cgcacccgga
     2161 cccggatcgc ggcgccgccg acctcgcgct cacgatcaac cgcgggcatc cgaagggcat
     2221 gcgcatctac atcgacctcg cctgggatct gcgctggacg gcggatgccg aggacggcgc
     2281 ccccggcatg cccgcgctcg cgcatgggat cgacatggac ctcgacgtcg tgcgcgtgga
     2341 gggggagcgc ggcacctggg tcgatgaccg cgacgagtgg gcggagcacc tggtgcagta
     2401 cgggtacccg gccgacgtga tcgcgcacct cgaggcgctc gcgctggacc tggagcagaa
     2461 ggtgcgcgcg caggtcgccc ccttcgacga cgccaccgcc gacccgtggc tcgaccgcct
     2521 ggaggcgctg ggcatggagc gccggcccga gaagtaggct ggacgggtga gcagcacccg
     2581 cctggtcgcg cagacccgcg agatcccacc cgtcgacgat ctgctggcac acacctccgc
     2641 cgccggaccc atcgcatggc tgcgccgcgg cgaggggatc gtggccgcag gcgacgggat
     2701 cgccacgacc gtccgcgtcc cggccgggc
//
LOCUS       DATMSX010000118         6882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_125066,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000118 DATMSX010000000
VERSION     DATMSX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125066"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VN041_03680"
     CDS             complement(<1..1477)
                     /locus_tag="VN041_03680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWU28179.1"
                     /translation="MRRLATYDSRDSAATIISAQTIRTVDEQRPQAEAMLTVGGRILA
                     VGSIAEVDAAAEAAGLQPVRVDHPGATIIPGFIDAHAHPLMYGQMLTWVDIGPDRAGS
                     IPEIVRLMTERAQTLPEGAPLRGYGYEQRNLAERRHPTRHELDEIATDREVYIMNASG
                     HGGVVNSFTLEKYGITRDTPNPEGGEFFRDADGELTGELSDAACNVLTGLHGVKVGHH
                     GPNFHLGDEPAEHQRQLDAVQREFLAGGVTAIGDAQVSQREFAAYLALADRGALDVRV
                     SMYFLSHLLDQVIDLGLTGPFGNAFLSVAGIKLYADGTLGGWTAYFPEGYVGDPCRTG
                     QLYHQPEEYAGLVKKAHLAGLQTATHAQSPTAIRMVVDALEEALTARPDADARHRIEH
                     CGLPDQAEIPRMAALGIRPVNQTQHYYNWGEGVEQAIGTPGERFNPLGEFLEAGVPVT
                     ISSDAPVAEPRPLEAIQAAVTRVTRRGHKLGPDSLRISAQDA"
     gene            complement(1481..2830)
                     /locus_tag="VN041_03685"
     CDS             complement(1481..2830)
                     /locus_tag="VN041_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28180.1"
                     /translation="MSTSAVQPAGHSHRRALRAGFAAMVGTSIEWYDFYVYATAAALV
                     FPQVFFPDVDRVTGTLASFGTYAVAFFMRPLGGIIFGHIGDKLGRKPALTVTLLMMGI
                     ATVLVGCLPGYATLGIAAPILLVLLRMVQGLAVGGEWGGASLMAVESAPPRFKVFYGG
                     FTQVGNPFGAMLATGAFWWLSTLGDEALTTWGWRVPFLFSIVLIGVGFWVRYRVEETP
                     VFEAKIEGHKQSTPVLFAVRNNWWPMLLGFCIIAMSSGGYVIATTFVENYSTSPEVGL
                     NSQTILAAMTIASFVELLITLPLAWLGDKWGSKTLIYIGAVGSIAVFVPLVLVIQGHQ
                     IAWIFVLASALRITLSGAWAPLSTLMAQMFRPQARYTSMSLSYGVGAAVWGGLSPAIA
                     TALIAGTGSFWAAVVFFGVLCILAVFGAYFAPQHSDNAPVTESLIARGTTAIRTVEG"
     gene            3076..4032
                     /locus_tag="VN041_03690"
     CDS             3076..4032
                     /locus_tag="VN041_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU28181.1"
                     /translation="MVSDDVRGRADRDVADLDSRLIHALQANGRSSIQDLARLLDAPR
                     DYVSQRLRALTDRGGLRVVAALDPGFAGHHVLTHAMVSLSGAARPVAEQIAEIDDAVF
                     VSLVSGARPLVFESRHGDESRLHEMLDQVRRIPTVQQIQVTTYAEVLKGFFVAESRTE
                     ITLDSLDERLIQALQSDGRASYQVLADTVHLSPSSARSRVRRLIDAGVIRISAIPAGG
                     LSRSRLAVGVGVTLRDDAEPVRRYIAEQRSIEFAARTHGLYDFILTIAGSSSAGVLSV
                     LEELRSLPQVGALENWAHLDIVKEDYARSLGLVVRSGSVGSP"
     gene            complement(4067..4735)
                     /locus_tag="VN041_03695"
     CDS             complement(4067..4735)
                     /locus_tag="VN041_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28182.1"
                     /translation="MIDVDAGGTDARARRRTWMLGGGLLAASALLNLWVASAPGAPVG
                     IIPDLLFAAGTVVFAIGLGQPGSVTARRPLGTGVLVGLGVWLLVKPMIHGLLMPPDPM
                     GDMVGWGARVQLMGMVVITLEVVALVLAILAVVQIGRSGVVPGPWNWAPLWVLAVVVA
                     TRLLTMGLLAVPVLTDSPDVLNAVISLAAFIGICAPGALGVLAMVLAARPVTGSTSVY
                     SSGE"
     gene            complement(4766..5251)
                     /locus_tag="VN041_03700"
     CDS             complement(4766..5251)
                     /locus_tag="VN041_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWU28183.1"
                     /translation="MTETMDETTKKHQDYNPGPRVGITFSTFDLLHAGHIMMLAEAKR
                     QCDYLICGLQMDPTLDRPEKNAPTQTVVERYIQLRGCEYVDEIVPYSTEQDLEDILRS
                     FKLDVRIVGDEYENRDFTGRSYCEEAGIELYFNSRNHRFSSSGLRKIVAAKEAERIAR
                     G"
     gene            complement(5307..6818)
                     /locus_tag="VN041_03705"
     CDS             complement(5307..6818)
                     /locus_tag="VN041_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HWU28184.1"
                     /translation="MTSTDAARRWYALPHPSTVRFEGGAWRPDTVQVIVADERLAAEA
                     ARLQRELAALGIPPGTGSRILLRSTEAPITSSDAASTPGGAVASDEAYAIDVGDDIDI
                     RVPSATGGFRATRQLLHNLRAQGCVPRGSVAGTPAVRERGVHLDAARKHYPAEWIIAL
                     LHAAADVGINVLQWHFSENEGFRLESAAFPEIVSAGHITRADAVRILDVARGLHVEVI
                     PSLDMPGHLRQVLAQHPHLRLPPGRERADAAITGTDHALDITREQAVAFAERLIDDVA
                     PVFEGCGRWHLGGDEFVDFARIDDYPALRDAAHARYGAEASGFDLLTAFVNRIAAHLR
                     GHGFRARAWNDGMLRGSVVRLDAGIELTWWTNWHPQMRPLADAVAAGHPLVNVNDGLF
                     YYVLGEHAGYRYPTAERIWAADWHPGLFPVLPGGGRQEIPHPYPDSLRGSCFAIWSDE
                     PGAQTVAEVADGVRAPLRAMAERAWNGGSALTLAEFERADAAIGLPEGASPPG"
BASE COUNT         1049 a         2399 c         2442 g          992 t
ORIGIN      
        1 gggcgtcctg ggcgctgatc cgcagggagt cgggcccgag cttgtggcca cggcgggtga
       61 cgcgcgtcac cgccgcctgg atggcctcca gcggacgggg ctcggcgacc ggggcatccg
      121 acgagatcgt gacggggacg cccgcctcga ggaactcgcc cagcgggttg aaccgctcac
      181 cgggggtgcc gatggcctgc tccacgccct cgccccagtt gtagtagtgc tgggtctggt
      241 tcaccgggcg gatgccgagc gcggccatgc gcgggatctc cgcctggtcg ggcaggccgc
      301 agtgctcgat ccggtgccgc gcgtcggcgt cggggcgggc cgtgagcgcc tcctccagcg
      361 cgtcgacgac catcctgatc gcggtgggcg actgcgcgtg cgtggccgtc tgcaacccgg
      421 cgaggtgcgc cttcttcacg agcccggcgt actcctcggg ctggtggtag agctgtccgg
      481 tgcggcacgg atcgccgacg tagccctccg ggaagtacgc ggtccatccc cccagggtgc
      541 cgtcggcgta gagcttgatg ccggccacgg agaggaatgc gttcccgaac ggaccggtca
      601 ggcccaggtc gatcacctgg tcgagcaggt gcgagaggaa gtacatcgac acccgcacgt
      661 cgagggcgcc ccggtcggcc agggccaggt aggcggcgaa ctcgcgctgc gacacctgcg
      721 cgtcgccgat ggcggtcacg ccgccggcga ggaactcccg ctgcacggcg tcgagctgcc
      781 gctggtgctc ggcgggctcg tccccgaggt ggaagttcgg gccgtggtgc ccgaccttca
      841 cgccgtgcag gccggtgagc acgttgcacg cggcatccga gagctcgccg gtcagctcgc
      901 cgtccgcgtc gcggaagaac tcgccgccct ccgggttcgg ggtgtcgcgg gtgatgccgt
      961 acttctccag cgtgaagctg ttcaccacgc cgccgtggcc ggaggcgttc atgatgtaca
     1021 cctcgcggtc ggtggcgatc tcgtcgagct catgacgggt ggggtgcctg cgctcggcga
     1081 ggttgcgctg ctcgtagccg tagccgcgca ggggggctcc ctccggcagc gtctgcgcgc
     1141 gctcggtcat gagccggacg atctcgggga tggatcccgc gcggtcggga ccgatgtcga
     1201 cccaggtcag catctggccg tacatcagcg ggtgcgcgtg cgcgtcgatg aagccgggga
     1261 tgatcgtcgc cccggggtgg tcgacgcgca cgggctgcag accggccgcc tcggctgcgg
     1321 cgtcgacctc ggcgatcgac cccacggcga ggatcctgcc accgacggtg agcatggcct
     1381 cggcctgcgg gcgctgctcg tcgacggtgc ggatggtctg agcggagatg atcgtcgcgg
     1441 cggagtcgcg ggagtcgtac gtggccagtc tgcgcatctc tcagccttcg acggtgcgga
     1501 tcgcggtggt gccgcgggcg atcagggact cggtgaccgg tgcgttgtcg gagtgctgcg
     1561 gggcgaagta cgcgccgaac acggcgagga tgcacagcac tccgaagaac acgaccgccg
     1621 cccagaagct gcctgtaccg gcgatcagcg cggtcgcgat ggccggggac agcccgcccc
     1681 acaccgcagc gccgacgccg tacgagagcg acatggacgt gtaccgcgcc tgcggccgga
     1741 acatctgcgc catcagggtg gacagcgggg cccaggcgcc gctgagcgtg atgcgcaggg
     1801 cggaggccag cacgaagatc caggcgatct ggtgcccctg gatcaccagc acgagcggta
     1861 cgaacacggc gatcgacccc accgcgccga tgtagatcag ggtcttgctg ccccacttgt
     1921 cgccgagcca ggccagcggc agggtgatca gcagctcgac gaacgacgcg atggtcatgg
     1981 ccgcgaggat ggtctgactg ttcaacccca cctcgggcga ggtcgagtag ttctccacga
     2041 acgtggtggc gatgacgtag ccgcccgacg acatggcgat gatgcagaag ccgagcagca
     2101 tcggccacca gttgttccgc accgcgaaca gcaccggcgt ggactgcttg tgcccctcga
     2161 tcttcgcctc gaacacgggc gtctcctcga cgcggtagcg cacccagaag ccgacgccga
     2221 tcagcacgat gctgaacagg aacgggacgc gccagcccca ggtcgtgaga gcctcgtcgc
     2281 cgagcgtgga gagccaccag aacgcgccgg tggcgagcat cgcgccgaac gggttgccga
     2341 cctgggtgaa cccgccgtag aagaccttga accgcggcgg cgcgctctcc accgccatca
     2401 ggctggcgcc accccactcg ccgccgacgg cgagcccctg caccatgcgc agcagcacga
     2461 gcaggatcgg cgcggcgatg cccagcgtgg cgtagccggg aaggcagccc accagcacgg
     2521 tcgcgatgcc catcatcagc agggtgacgg tcagcgccgg cttgcggccg agcttgtcgc
     2581 cgatgtgccc gaagatgatc ccgccgaggg gccgcatgaa gaacgcgacc gcgtaggtgc
     2641 cgaacgacgc cagggttccg gtgaccctgt cgacgtcggg gaagaacacc tgcgggaaga
     2701 cgagggcggc ggcggtcgcg tagacgtaga agtcgtacca ctcgatcgag gtgccgacca
     2761 tggcggcgaa accggcgcgg agcgcgcggc ggtgcgaatg ccccgccggc tggactgcgg
     2821 aggtggacat acagggctcc tttgctctgt cgaagggtaa gcggcagtga ggggctgcag
     2881 catcgggatg agccgactat aggcccctct tcaccgcaga tccacgcgtg cggagggcag
     2941 ttgaccaccg atctgcggta agatcaccgc tgaacgtgtt cactcgcggg ccgtcggcac
     3001 cctctcgccg acgatctact gctccggatc cgcagatccg gggacttcgc ggatgttcgg
     3061 aatcaggagg gcgacgtggt ctccgacgat gttcgcgggc gggccgaccg tgacgtggcg
     3121 gacctggaca gcaggctgat ccacgcgctg caggccaacg gccggtccag catccaggat
     3181 ctcgcccgtc tcctggacgc accccgcgac tacgtgtccc agcgtcttcg cgccctcacc
     3241 gaccgcggcg ggctgcgggt ggtcgcggcg ctcgaccccg gcttcgccgg ccaccacgtg
     3301 ctcacgcacg cgatggtgag cctgagcggc gccgcccgac ccgtcgccga gcagatcgcc
     3361 gagatcgacg acgcggtgtt cgtgtcgctc gtgagcggtg cgcgtccgct cgtcttcgag
     3421 tcccggcacg gcgacgagag ccggctgcac gagatgctcg accaggtgcg ccggatcccc
     3481 acggttcagc agatccaggt caccacgtac gccgaggtgc tcaagggctt cttcgtcgcc
     3541 gagtcgcgca ccgagatcac gctcgacagc ctggacgagc ggctcatcca ggcactgcag
     3601 tccgacggcc gcgccagcta tcaggtgctc gccgacaccg tgcacctctc gccttcgtcg
     3661 gcgcggtcgc gcgtgcgccg gctcatcgac gccggcgtca tccgcatctc ggccatcccc
     3721 gcgggcggcc tctcccgaag ccggctcgcg gtcggcgtcg gagtcacgct gcgcgatgac
     3781 gccgagccgg tgcgccggta catcgcggag cagcgatcga tcgagttcgc ggcccggacg
     3841 cacgggctct acgacttcat cctgaccatc gccgggtcgt cgtcggccgg ggtgctctcc
     3901 gtgctcgagg agctgcgctc cctcccgcag gtgggggctc tggagaactg ggcgcacctc
     3961 gacatcgtga aggaggacta cgcccgctcg ctcgggctgg tggtgcgttc cggctccgtc
     4021 ggcagtccct gaaccatgcc gcgccgcacg accgcccgct gagcgctcac tcccccgagc
     4081 tgtacacgct cgtcgatccg gtcaccggcc gggcggccag caccatggcg agcacaccca
     4141 gagcaccggg cgcgcagatg ccgatgaagg cggccaggct gatcactgcg ttcagcacgt
     4201 cggggctgtc cgtcaggacg gggacggcga gcaggcccat cgtcagcagc cgggtggcca
     4261 ccacgacggc gagcacccac aacggtgccc agttccacgg ccccggcacg acgccgctgc
     4321 gaccgatctg caccacggcg aggatcgcca gcaccagcgc gaccacctcc agggtgatga
     4381 ccaccatgcc catcaactgg acccgcgcac cccagccgac catgtcgccc atcggatccg
     4441 gtggcatgag cagcccgtgg atcatcggct tcacgagcag ccacaccccg agcccgacca
     4501 gcacgcccgt gccgagcgga cggcgcgccg tgacgctgcc cggctgcccc agcccgatcg
     4561 cgaagacgac cgtgccggcg gcgaagagca ggtcgggaat gatcccgacc ggcgcgccgg
     4621 gagccgaggc gacccagagg ttcagcagcg cggatgccgc cagcagacca ccaccgagca
     4681 tccaggtgcg tcgacgcgca cgggcatccg tcccgcctgc gtccacgtcg atcatgccgc
     4741 caggctaccg ccaggcacgc tcgattcagc cgcgcgcgat ccgctccgct tccttggcgg
     4801 cgacgatctt gcgcagcccg gagctggaga agcggtggtt gcggctgttg aagtacagct
     4861 cgatgccggc ctcctcgcag tacgagcggc cggtgaagtc gcggttctcg tactcatcgc
     4921 cgacgatgcg cacgtcgagc ttgaaggagc gcaggatgtc ctcgaggtcc tgctcggtgg
     4981 agtacggcac gatctcgtcg acgtactcgc agccgcgcag ctggatgtag cgctccacga
     5041 cggtctgcgt cggggcgttc ttctccgggc ggtcgagcgt cgggtccatc tgcaggccgc
     5101 agatcaggta gtcgcactgg cgcttggcct cggcgagcat catgatgtgg cccgcgtgca
     5161 gcaggtcgaa ggtcgagaag gtgatgccca ctcgaggacc cgggttgtag tcctggtgct
     5221 tcttcgtcgt ctcgtccatg gtctccgtca tcgcttcgct ccggtacgcc gtttcaccgc
     5281 gtccccacaa cacggccaca gggattctac ccgggtgggc tggcaccctc gggcaggccg
     5341 atcgcggcat ccgcccgctc gaactccgcc agggtcagcg cggagccgcc gttccacgcc
     5401 cgctccgcca tggcccggag cggtgcgcgg acgccgtcgg cgacctccgc gacggtctgc
     5461 gcccccggct cgtccgacca gatggcgaag cagctcccgc gcagcgagtc cgggtagggg
     5521 tgcgggatct cctgcctgcc gcctcccggc agtaccggga acagtccggg atgccagtcc
     5581 gccgcccaga tccgctccgc ggtcgggtac cggtagccgg cgtgctcgcc gaggacgtag
     5641 tagaacagcc cgtcgttgac gttgaccagc ggatgccccg cggcgacggc atccgccagg
     5701 ggacgcatct gcggatgcca gttcgtccac caggtgagct cgatgccggc atccaggcgc
     5761 acgacggagc cgcgcagcat gccgtcgttc catgcccggg cccggaaccc gtgtccgcgc
     5821 aggtgtgcgg cgatgcggtt cacgaaggcg gtgagcagat cgaatccgct cgcctcggca
     5881 ccgtagcgcg catgcgcggc gtcgcgcagt gccgggtagt cgtcgatgcg ggcgaagtcg
     5941 acgaactcgt cgccgccgag atgccagcga ccgcagccct cgaacacggg cgccacgtcg
     6001 tcgatcagcc tctcggcgaa ggccacggcc tgctcgcgag tgatgtccag cgcatggtcc
     6061 gtgccggtga tcgcggcatc cgcccgttcc cggcccggcg gcaaccgcag gtgcggatgc
     6121 tgtgccagca cctgccgcag atggccgggc atgtccagcg aggggatcac ctcgacgtgc
     6181 aggccgcggg cgacatcgag gatgcgcacc gcatcggcgc gggtgatgtg cccggccgac
     6241 acgatctcgg ggaacgcggc ggattccagc cggaacccct cgttctcgga gaagtgccac
     6301 tggaggacgt tgatgcccac gtcggccgct gcgtgcagca gggcgatgat ccactcggcc
     6361 gggtagtgct ttcgggcggc gtcgagatgg acaccgcgtt cgcggacggc aggcgttccg
     6421 gcgacggagc cccggggaac acacccctgc gcacgaaggt tgtgcagcag ctgccgggtg
     6481 gcgcggaagc cgcccgtcgc cgaggggacg cggatgtcga tgtcgtctcc gacatcgatc
     6541 gcgtaggcct cgtcggatgc gacggctccc cccggagtcg aggccgcatc cgacgaggtg
     6601 atcggggcct ccgtcgaccg caggaggatg cgcgatcccg tgccgggagg gatgcccagt
     6661 gcggcgagct cgcgctgcag gcgggccgcc tcggcagcca ggcgctcatc cgcgacgatc
     6721 acctgcacag tgtccggccg ccaggctccg ccctcgaacc ggacggtcga cggatgcggc
     6781 agcgcgtacc agcggcgtgc cgcatccgtc gatgtcatca gagcgccctg cgggtcagcc
     6841 gcacgccggc acggcgtagg tgcggtcgac ggtcatggcg cc
//
LOCUS       DATMSX010000119         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1829,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000119 DATMSX010000000
VERSION     DATMSX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VN041_03710"
     CDS             complement(<1..837)
                     /locus_tag="VN041_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28185.1"
                     /translation="MTALLEVQNLSLEFRSQGAPPIAALQDVSFTLDRGETLAVLGES
                     GSGKSITAKAIMGILPRPASHITGGRILFDGDDLLTLSAARLRKVQGARIAMVFQDAL
                     SALNPVQTVATQIAELYRVHRGMPKKQAHEAAVDMMRAVRIPDAANRANDYPFQFSGG
                     MRQRIMIAMALALEPDILIADEPTTALDATVQAQILELLGEITQERRMALLMITHDLG
                     VAGQLADRALVMYAGRVAESGPIGPMFDNPAHPYTKGLLGSIPNAAHRGGDLPTIQGL
                     PPD"
     gene            complement(834..1724)
                     /locus_tag="VN041_03715"
     CDS             complement(834..1724)
                     /locus_tag="VN041_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28186.1"
                     /translation="MSESTTSLIISHRRRGRPRSGLRSLWKSPFFDIAALIIIVVTLM
                     ALFPTLFTNIDPRDCDLSMSRVGPEAGHPMGFDIQGCDYWSNIVHGARTSLEVGVYTA
                     AISFVIALIAGSLAGYFGGVTDSIISWTSNVILGIPVGIASLVILYQFRSRTVWTIVF
                     VLVLFSWAATMRYMRSSVMQVRNLEYIQAARGLGVGPWRILRSHVIPNAVTPLIVMTT
                     LSVGAGMAAEAGFSILGVGLKLPAFSWGIQIALASQDGNWQIAPLLMFFPTLMLGLTT
                     LAFVVLGESLRDALDPKARR"
     gene            complement(1717..2646)
                     /locus_tag="VN041_03720"
     CDS             complement(1717..2646)
                     /locus_tag="VN041_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28187.1"
                     /translation="MLRYALRRILMMVPTLIGSTLLVYLLVFLIPGDPVARLAGGEKR
                     LSDSTIAAIRAQYNLDDPFFVQYAKYLWGVLQGDFGHDFNGNSVSTLIATSLPYTVVL
                     ALSAFVLKLIIGVCLGVWAGLRQGKFPDKFNLFFTIFFLAVPGFIIAYFSQYLFGIKL
                     HWLPISGVRAGFPLAFIMPAMVLALETASPLARLSRTSLVDVLRSDYIVTATAKGIGP
                     QRIMWVHALRNAFMPVVTYLGLSMAALLGGSVLIETIFNLPGLGGTLARAIDTQNGPV
                     VVGVSVFLLLFYLVAALIVDLLYPIIDPRVRHV"
     gene            complement(2646..>4203)
                     /locus_tag="VN041_03725"
     CDS             complement(2646..>4203)
                     /locus_tag="VN041_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU28188.1"
                     /translation="DPADLRGLAALESLSAAMTPAMAALLLTADPDVIVLGGELGEFA
                     DLIAGPLAEGIRAAVTGIDTGTRIIRGGELGADAAALGALGLAFEKHSSRIYGVPDVP
                     APVIAIEPENEKMTTDIASEQAPFRAAAIGVGARVGLALHVKEVGGTVVAAVDPDPRT
                     HERAARLFGDIPVFSSVAELLASGIEIDGAFITTPDFTHAEVAIELLEAGIPSYIEKP
                     LAISIEDADRILETAYRTGTKVYVGHNMRHMQVVRMMKEIVDRGEIGEVKAIWCRHFV
                     GHGGDYYFKDWHAERDKAMSLLLQKGAHDIDVMHWIAGTSTRSVVGVGDLAVYGDVTS
                     RRDNSDRRMGDWFSMQNWPPASQQDLNPVIDVEDISMIMMRTDSGVLMSYQQCHFTPD
                     YWRNYTVIGTEGRIENFDDDAGGEIRLWNARSDYLEKGHVRYPIEDGGDGGHGGADPL
                     TVAEFVEFVRTGAPTDTSPVAARDAVAAGIGGALSIRSHSEPFEIPRLSDEMLEYYAA
                     HQPARTPVGD"
BASE COUNT          716 a         1348 c         1482 g          657 t
ORIGIN      
        1 gtccggcggc aggccctgga tggtgggcag gtcgcctccc cggtgcgcgg cgttagggat
       61 ggagccgagc aggcccttcg tgtacggatg cgcggggttg tcgaacatcg ggccgatcgg
      121 accggactcg gcgacgcggc cggcgtacat caccagcgcg cggtcggcga gctggccggc
      181 gacgcccagg tcgtgcgtga tcatcagcag ggccatccgc cgctcctgcg tgatctcgcc
      241 gagcagctcc aggatctggg cctgcaccgt ggcgtccagc gccgtggtcg gctcgtccgc
      301 gatgaggatg tcgggctcca gggccagtgc catcgcgatc atgatgcgct ggcgcatgcc
      361 gcccgagaac tggaacggat agtcgttcgc gcggttcgcg gcatccggga tgcggacggc
      421 gcgcatcatg tcgaccgcgg cttcgtgtgc ctgcttcttc ggcatgccgc gatgcacgcg
      481 gtagagctcg gcgatctggg tggccaccgt ctgcacgggg ttcaacgcgc tcagtgcatc
      541 ctggaacacc atcgcgatgc gcgccccctg caccttgcgc agcctcgcgg cggacagggt
      601 cagcaggtcg tcgccgtcga acaggatgcg tccaccggtg atgtgcgagg cgggcctggg
      661 caggatgccc atgatcgcct tggcggtgat gctcttgccg gagccggact cgccgagcac
      721 ggccagcgtc tcaccacggt ccagcgtgaa gctgacgtcc tgcagcgccg cgatcggcgg
      781 ggccccttgc gagcggaact cgagggacag gttctggact tccagcaggg cggtcatcgg
      841 cgggccttcg ggtcgagggc gtcgcgcagg ctctcaccca gcaccacgaa ggcgagcgtg
      901 gtgaggccca gcatcagtgt ggggaagaac atcagcagcg gtgcgatctg ccagttgccg
      961 tcctggctcg cgagggcgat ctggatgccc caggagaacg ccggcagctt cagcccgaca
     1021 ccgaggatcg agaatcccgc ctctgcggcc ataccggctc cgaccgagag cgtcgtcatc
     1081 acgatcagtg gagtgacggc gttcgggatc acatgcgagc gcaggatgcg ccacggtccg
     1141 acgcccaggc cgcgtgcggc ctggatgtac tccagattgc gcacctgcat gacggagctg
     1201 cgcatgtatc gcatggtcgc cgcccagctg aacagcacca gcacgaacac gatggtccac
     1261 accgtgcggg accggaactg gtacaggatg accaggctgg cgatgcccac ggggatgccg
     1321 aggatgacat tcgacgtcca ggagatgatc gaatccgtga cccctccgaa gtagcccgcg
     1381 agcgacccgg cgatcagggc gatgacgaac gagatcgcgg cggtgtagac gccgacctcg
     1441 agggaggtgc gcgcgccgtg gacgatgttc gaccagtagt cgcagccctg gatgtcgaag
     1501 cccatcggat gtccggcttc cggtccgacc cgcgacatgg acagatcgca gtcccgcgga
     1561 tcgatgttcg tgaacagcgt cgggaacagt gccatcagcg tgacgacgat gatgatcagc
     1621 gcggcgatgt cgaagaacgg gctcttccac aggctgcgca gcccgctgcg cggacgaccg
     1681 cggcgacgat ggctgatgat gagcgaggtg gtggactcag acatggcgca ccctcgggtc
     1741 gatgatcggg tagagcaggt ccacgatcag cgcggcgacc aggtagaaca gcagcaggaa
     1801 caccgagacg ccgacgacca cggggccgtt ctgcgtgtcg atggcccgcg ccagtgtgcc
     1861 accgaggccg gggaggttga agattgtctc gatgagcacc gaaccgccca gcagcgcggc
     1921 catgctcaga ccgagatagg tgaccaccgg catgaacgcg ttgcgaagtg cgtgcaccca
     1981 catgatgcgc tgcgggccga tccccttcgc cgtcgcggtg acgatgtagt ccgatcgcag
     2041 cacgtcgacg aggctcgtgc ggctgagacg cgccaggggc gaggccgtct ccagtgcgag
     2101 caccatcgcc ggcatgatga acgccagtgg gaacccggct ctcaccccgg atatcggcag
     2161 ccagtgcagc ttgatgccga acaggtactg ggagaagtac gcgatgatga atccgggcac
     2221 ggccaggaag aagatcgtga agaacaggtt gaacttgtcc gggaacttcc cctgccggag
     2281 acccgcccag acacccaggc acacgccgat gatgagcttc agcacgaagg ccgacagcgc
     2341 gagcaccacg gtgtagggca gcgaggtcgc gatcagggtc gagaccgagt tgccgttgaa
     2401 gtcgtggccg aagtcgccct gcagcactcc ccagaggtac ttcgcgtact gcacgaagaa
     2461 cggatcgtcg aggttgtact gcgcgcggat cgccgcgatg gtcgagtccg agagccgctt
     2521 ctcgccgccg gcgagcctgg cgaccggatc accggggatg aggaagacga gcaggtagac
     2581 gaggagcgtc gacccgatca gggtcggcac catcatcagg atgcgccgca gcgcgtaccg
     2641 gagcatcagt ccccgaccgg tgtgcgggcc ggctggtgcg ccgcgtagta ctcgagcatc
     2701 tcgtcgctca gccgcgggat ctcgaagggc tccgagtggg agcggatcga cagtgcgcct
     2761 ccgatgccgg cagcgaccgc atcgcgcgcg gccaccgggc tggtgtccgt cggcgctccg
     2821 gtgcggacga actcgacgaa ctccgcgacc gtgagcgggt cggcaccgcc gtgacccccg
     2881 tcgccgccgt cctcgatcgg gtagcgcacg tgccccttct cgaggtagtc gctgcgggcg
     2941 ttccagagcc ggatctcgcc gccggcgtcg tcatcgaagt tctcgatgcg accctcggtg
     3001 ccgatcaccg tgtagttgcg ccagtagtcc ggtgtgaagt ggcactgctg gtagctcatg
     3061 agcacgccgg aatcggtgcg catcatgatc atcgagatgt cctcgacgtc gatgaccggg
     3121 ttgaggtcct gttgggatgc cggtggccag ttctgcatcg agaaccagtc gcccatccgg
     3181 cggtcggagt tgtcgcggcg ggaggtgacg tctccgtaca ccgcgaggtc gccgacgccg
     3241 acgacggagc gcgtggaggt gccggcgatc cagtgcatca catcgatgtc gtgcgcaccc
     3301 ttctgcagca gcaggctcat ggccttgtcc cgctcggcgt gccagtcctt gaagtagtag
     3361 tcgccaccgt gtccgacgaa gtgccggcac cagatcgcct tgacctcacc gatctcgccg
     3421 cggtcgacga tctccttcat catgcgcacg acctgcatgt gccgcatgtt gtgaccgacg
     3481 tagaccttgg tgccggtgcg gtacgcggtc tcgaggatcc ggtcggcgtc ctcgatcgag
     3541 atcgcgaggg gcttctcgat gtacgacggg atgcccgcct cgagcagttc gatcgccacc
     3601 tcggcgtgcg tgaagtccgg ggtggtgatg aacgcgccgt cgatctcgat gccggaggcg
     3661 agcagctcgg cgacggagga gaacaccggg atgtccccga acagccgcgc tgcgcgctca
     3721 tgcgtgcggg ggtcgggatc caccgcggcg acaacggtgc cgcccacctc cttcacgtgc
     3781 agcgcgaggc cgacgcgagc accgactccg atcgctgcgg cacggaaggg ggcctgctcc
     3841 gaggcgatgt cggtggtcat cttttcgttc tccggttcga ttgcgatgac gggcgccgga
     3901 acgtccggaa ccccgtagat gcgggaggag tgcttctcga aggcgaggcc gagggctccg
     3961 agcgccgcgg catccgctcc caactcgccc ccgcggatga tgcgcgtgcc ggtgtcgatg
     4021 ccggtcaccg ccgcacggat gccctcggcg agcggacccg cgatgaggtc ggcgaactca
     4081 ccgagctctc cgccgagcac gatcacgtcg ggatcggccg tgagcagcag cgctgccatc
     4141 gccggggtca tcgcggcgct gagggactcg agcgcggcca ggccgcgcag gtcggccggg
     4201 tcg
//
LOCUS       DATMSX010000120         7568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1852,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000120 DATMSX010000000
VERSION     DATMSX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VN041_03730"
     CDS             complement(<1..1060)
                     /locus_tag="VN041_03730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWU28189.1"
                     /translation="MTLPPRRIRRRIPSWGAVAPLLGFRRPVWDRVDRRLAQAMTVKD
                     LARIARRTTPRSAFDYVHGAAEDELSIARARRAFASVEFEPRVLHDVASVDTSTMILG
                     RRASFPLILAPTGFTRMMQHEGEIAVARAAARAGVPYTLSTMGTTSPAELTDAVPSGD
                     NWFQLYLWKDRDGTRRLIEEARRTGYETLVLTVDTPVAGNRLRDARNGLTIPPTLNLK
                     TMWDMAWHPAWWFNVLTTDPIEFASLRSFDGTVAELVAKMFDPSLKIEDLAWLREEWS
                     GKLVVKGIQSVADAQRVVDAGADALVVSNHGGRQLDRAPTPLRLLPEVATAVGDRAEI
                     FLDTGILSGADVLAAVGLG"
     gene            complement(1057..1782)
                     /locus_tag="VN041_03735"
     CDS             complement(1057..1782)
                     /locus_tag="VN041_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28190.1"
                     /translation="MTEPLLSVKDLSVSYGRVHAVRGISFDVAVGTLVTLVGANGAGK
                     SSIINAISGIVRPSGGRILFDGEDITKTKSHRLVPRGLVQVPEGRQILATMTIAENLQ
                     MGARSYGAGAAAGIDEMYQRFPVLGERRKLAAGSLSGGEQQMLAIARAMLARPKLILM
                     DEPSMGLAPKIVNEVFGVIDEVLAGGTTVLLVEQNARRALQAAQEGHILQNGEIVRSG
                     ASETLLADDDIIQAYLGTGRAKE"
     gene            complement(1775..3505)
                     /locus_tag="VN041_03740"
     CDS             complement(1775..3505)
                     /locus_tag="VN041_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWU28191.1"
                     /translation="MLFTESTLVFIALNGVFAFSFYAVLVAGQLSLAQAGFAGIAGFT
                     AAALTPPPLQWGPIPTLLFAMVIGMVVGAITAVILGLPTMRLRGVYLAIATLGFAEAI
                     RIFLLNSDWTGGAQGMSVPKTLTPAVAWVVLAIVTYWFARQSRSRYGRALEAIREDEL
                     AARSIGINVGGHRLSAFVAAGVLAGLYGVMWAYYVRLMAPEDFGFSTAIDGLVTAVVG
                     GTANFIGPILGSGFQTMLPEVLRVTGVEASWIRPFISGLALLLVILFLPGGIASLIPR
                     RRQKVDVAALQTDATQLSLRTHPAAGEVVVELKQLAKEYGGVHAVRGVDLTVRSGEVV
                     GLIGPNGAGKTTLVNMVSGLIPPSSGTATVLGVPIGRTPVHRIAAAGVTRTFQHSKLF
                     NRLTALENVLIGAHVVAKPTFLRRLIWLPSARRDERVALAHAARCLDRVGLLDKANTL
                     AKGLSYGDQRRLEIARALAADPSLLILDEPAAGMNHVEADGLSALIKDLSADGLTIIF
                     IEHNVGMVLDTCDHIVVLNFGEVLADGTPDQIAADERVIEAYLGAADEPDADDTTSAP
                     AQPAEENSND"
     gene            complement(3505..4383)
                     /locus_tag="VN041_03745"
     CDS             complement(3505..4383)
                     /locus_tag="VN041_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU28192.1"
                     /translation="MLQQLLNGLFLGSIYSLFAIGFTLVFGILDRLNLAHPSVFAASA
                     FVGIWLTDAFDLPIWLVLILVFAFGAVMGLLIERVAFRPLKDRPDAHFAGLISSIAMA
                     GMIVALLQAAFGPNTRRFPVGTIPEDALHMGDLTASWLQIIILVVSLGLMIALSLLVQ
                     RSSLGRSMRAVAENPTAARVLGVDVDRVTALTFAISTALGAVAGVLFALNVNSAQLGM
                     GSAIELKGLAVIIVGGMGSLSGSLVGGLVLGIAEVFAIQYIGSSWRDLIAFALLFVIL
                     LVRPEGLFGKRKVREV"
     gene            complement(4422..5597)
                     /locus_tag="VN041_03750"
     CDS             complement(4422..5597)
                     /locus_tag="VN041_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28193.1"
                     /translation="MRKMIRNAALLSASVLLLAGCSATGGGTGGDAGGGLKGSGSGDS
                     CTIDGTVPLGAALSLTGAAASYGESQQNGLKLALKDLNAKKGVKYDLKIEDDGTDPKQ
                     AIGVFEKFVADSTSAVIGPTLSNTAFQAQPIAQDAGMPVLAISNTAAGITAQGDYIFR
                     DSLTEGQVIPQTIKAAADKFDLKKVVVMYSNDDAFTESGYKVMADSLKKEGIDAVDTL
                     KFSKSDTDFRSLLTAAKEKKPDAIVVSALIEAAIPLVTQARELGITVPIIGGNGFNNP
                     QLMKDAGPAAEGVVVGAAWNSASESPENTAFMKAYEDEYGSGPDQFAAQAYTGLLVLD
                     HAVRANCSGERADIQKGLNEVKDLKTPLGSLTINKDRDAEHNAVVQIVKDGKFAVLD"
     gene            complement(5783..6142)
                     /locus_tag="VN041_03755"
     CDS             complement(5783..6142)
                     /locus_tag="VN041_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein"
                     /protein_id="HWU28194.1"
                     /translation="MGTLREIVVFLHIIGFATLFGAWATQAFGGKKQFTQLMNVGLAI
                     AGIAGLLLAWPMGLFEPGAGFYIKITVKLVVLIVIGALIGIGMARQKRGEAVPAGMFW
                     SVGVLALLNAGLGLFWH"
     gene            complement(6241..6717)
                     /gene="ribH"
                     /locus_tag="VN041_03760"
     CDS             complement(6241..6717)
                     /gene="ribH"
                     /locus_tag="VN041_03760"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWU28195.1"
                     /translation="MSGAGAPTPLTPVDGTGLRVVVIAGTWHEVISNGLIAGAQRTLD
                     AAGAEHTLVRVPGSFELAVAAQAAFAGGADAVVALGVIIRGGTPHFEYVSAATTDGLT
                     RVSLDAGKPVGFGLLTLDDEQQGLDRAGLPGSQEDKGAEAADAALRTARVIADLRG"
     gene            complement(6714..>7568)
                     /gene="ribA"
                     /locus_tag="VN041_03765"
     CDS             complement(6714..>7568)
                     /gene="ribA"
                     /locus_tag="VN041_03765"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWU28196.1"
                     /translation="GGHTEAAVELMRLAGLHPVGAIAEVVAEDGSMMRLPALQELGAR
                     EDIPVITIEQLIRHLNEVDPRDEADCRAKKTARRVSLRADATVPTEHGTFRFLAYKDR
                     VTGTDHIAVVSGTPGETALVRVHSECLTGEAFGSQKCECGPQLQAALDRIDAEGGVVI
                     YMRGHEGRGIGLINKLRAYSLQEEGLDTVDANLALGLPADARDYAAAAGILSDLGVSK
                     VRLLTNNTDKVKQLRELGLDVVEQVPLIVGVGPNNHQYLETKRDRMGHIIGESELAEA
                     LAHGKDDA"
BASE COUNT         1203 a         2540 c         2654 g         1171 t
ORIGIN      
        1 cgcccaggcc gacggctgcc agcacgtcgg cgccggacag gatgccggtg tcgaggaaga
       61 tctcggcgcg gtcaccgact gcggtggcga cctcgggcag cagccgaagg ggagtgggcg
      121 cgcggtcgag ctggcggccg ccgtggttcg agacgacgag tgcatcggcg ccggcgtcca
      181 cgactcgctg tgcgtcggcg acggactgga tgcccttgac cacgagcttt ccggaccact
      241 cctcgcgcag ccacgcgagg tcctcgatct tcagtgaggg gtcgaacatc ttcgcgacca
      301 gctcggccac ggtgccatcg aacgagcgca ggctggcgaa ctcgatcgga tcggtggtga
      361 gcacgttgaa ccaccaggcc ggatgccagg ccatgtccca catggtcttg agattcagcg
      421 tcggcggtat cgtcaggccg ttgcgtgcgt cgcgcagacg gttccccgcc acgggggtgt
      481 cgaccgtgag cacgagcgtc tcgtacccgg tgcgacgggc ctcctcgatc agacggcggg
      541 tgccgtcgcg gtccttccac aggtacagct ggaaccagtt gtcgccgctc ggtacggcat
      601 ccgtcagctc ggcgggcgac gtcgtgccca tcgtcgacag cgtgtacgga acccccgcgc
      661 gcgccgccgc cctggccacc gcgatctcgc cctcgtgctg catcatgcgg gtgaagccgg
      721 tgggtgccag gatcagcgga aaggatgccc gtcgtccgag gatcatggtc gacgtgtcca
      781 cggacgcgac gtcgtgcaga acccgcggtt cgaactccac gctcgcgaac gcccggcggg
      841 cgcgggcgat gctcagttcg tcctctgcgg cgccgtgcac gtaatcgaac gccgaccgcg
      901 gcgtggtgcg ccgcgcgatg cgggcgaggt ccttcaccgt catcgcctgc gcgagccgac
      961 gatccacgcg atcccacacc ggtcgccgga atccgagcag gggagccacg gcaccccagc
     1021 tcgggatgcg acggcggatc cggcgcggcg gcagcgtcat tccttcgccc gcccggtgcc
     1081 gaggtacgcc tggatgatgt cgtcgtcggc gagcagcgtc tccgatgcac ccgagcggac
     1141 gatctcgccg ttctgcagga tgtgcccctc ctgggcggcc tggagcgcac gacgcgcgtt
     1201 ctgctccacg agcagcacgg tcgtccctcc tgcaagcacc tcgtcgatca caccgaacac
     1261 ctcgttgacg atcttgggtg ccagacccat ggacggctcg tccatcagga tcagcttcgg
     1321 tcgcgcgagc atggcgcgtg cgatggccag catctgctgc tcgccaccgg agagagagcc
     1381 cgcggccagc ttgcgccgct cgcccagcac ggggaagcgc tggtacatct cgtcgattcc
     1441 ggccgcggcg cccgcgccat aggagcgcgc gcccatctgc aggttctcgg cgatcgtcat
     1501 ggtcgccagg atctgccggc cctcggggac ctgcaccagc ccgcgcggca ctagccggtg
     1561 cgacttggtc ttcgtgatgt cctcgccgtc gaacaggatc cgcccgcctg acgggcgcac
     1621 gatgccggag atcgcgttga tgatcgagga tttgcccgcg ccgttcgcgc cgaccagcgt
     1681 caccagggtg ccgaccgcga catcgaagga gatgccgcgc acggcgtgca ctcgtccgta
     1741 tgagaccgac aggtccttca cactcagcag cggctcagtc attgctgttc tcctccgcgg
     1801 gctgcgcggg ggcggaagtt gtgtcgtccg catccggttc gtccgccgca cccaggtacg
     1861 cctcgatcac gcgctcgtcc gcggcgatct ggtccggagt gccgtcggcg agcacctcgc
     1921 cgaagttcag caccacgatg tgatcgcagg tgtccagcac catgccgacg ttgtgctcga
     1981 tgaagatgat cgtcagcccg tcggcggaca ggtccttgat caacgcgctg aggccgtcgg
     2041 cctcgacgtg gttcatgccg gcggccggct cgtccaggat cagcagcgac gggtcggcgg
     2101 ccagcgcgcg ggcgatctcc aggcggcgct gatctccgta ggagaggccc ttggcgagcg
     2161 tgttcgcctt gtcgagcagg cccaccctgt cgaggcagcg tgcggcgtgc gcgagcgcga
     2221 cgcgctcgtc gcgacgggcc gagggcaacc agatcagccg gcgcaggaat gtgggcttgg
     2281 cgacgacgtg cgcgccgatc agcacgttct ccagcgcggt caggcggttg aacagcttgg
     2341 agtgctggaa ggtgcgggtg acgcccgcag ccgcgatcct gtgcacgggg gtgcgcccga
     2401 tcggaacgcc gaggaccgtc gcggtacccg acgacggcgg gatcagaccc gagaccatgt
     2461 tcaccagggt ggtcttgcct gcgccgttcg gtccgatcag gccgaccacc tctccgctgc
     2521 ggaccgtgag gtcgacgccg cgcacggcgt gcactccgcc gtactccttg gcgagctgct
     2581 tcagttcgac gacgacctcg cccgcggcgg ggtgcgtgcg cagcgacagc tgggtggcat
     2641 ccgtctgcag agccgccacg tcgaccttct gccggcggcg cgggatcagg ctcgcgatgc
     2701 cgccgggaag gaacaggatc accaacagca gggccaggcc ggagatgaac gggcggatcc
     2761 agctcgcctc gacgcccgtg acgcgcagca cctcgggcag catggtctgg aatccgctgc
     2821 cgaggatggg gccgatgaag ttcgcagtgc caccgacgac tgcggtgacc aggccatcga
     2881 tcgcggtgct gaatccgaag tcctccggtg ccatgagccg cacgtagtac gcccacatca
     2941 cgccgtacag gcctgcgagc acgcctgcgg cgacgaacgc ggacaggcga tgcccgccga
     3001 cgttgatgcc gatcgagcgt gcggcgagct cgtcctcgcg gatcgcctcg agggcccggc
     3061 cgtaccgcga gcggctctgc cgggcgaacc agtaggtgac gatcgccagc acgacccagg
     3121 cgacggccgg ggtgagcgtc ttgggcaccg acatgccctg ggcgccgccg gtccagtcgg
     3181 agttcagcag gaagatgcgg atggcctctg cgaacccgag ggtcgcgatc gcgaggtaca
     3241 cgccgcgcag cctcatggtg ggcaggccca ggatcacggc cgtgatcgca ccgaccacca
     3301 ttccgatcac catcgcgaac agcagggtcg ggataggccc ccactgcagg ggagggggag
     3361 tgagtgcggc ggccgtgaag ccggcgatgc cggcgaatcc ggcctgcgcc aggctcagct
     3421 gaccggcgac gagcaccgcg tagaacgaga acgcgaagac gccgttgagc gcgatgaaga
     3481 cgagggtcga ctcggtgaaa agcatcagac ctcacgcacc ttgcgcttgc cgaacaggcc
     3541 ctcgggccgg accagcagga tcacgaacag cagtgcgaag gcgatcagat cgcgccagct
     3601 ggatccgatg tactggatgg cgaagacttc ggcgatgccg aggacgaggc cacccaccag
     3661 cgaaccggac aacgacccca taccgccgac gatgatcacg gcgagtccct tcagctcgat
     3721 cgcgctgccc atgcccagct gcgcgctgtt cacgttcagg gcgaacagca cgcctgcgac
     3781 cgcgccgagc gccgtcgaga tggcgaacgt cagcgcggtg acccggtcga cgtccacgcc
     3841 caggacgcgc gccgcggtcg ggttctccgc cacggcgcgc atcgagcggc ccagggacga
     3901 ccgctgcacg agcaggctca gagcgatcat caggcccagg gacaccacca ggatgatgat
     3961 ctgcagccag ctcgccgtga gatcgcccat gtgcagcgcg tcctcgggga tggtgcccac
     4021 cgggaaccgt cgcgtgttgg ggccgaaggc cgcctgcagc agggcgacga tcatcccggc
     4081 catcgcgatc gacgagatca gacccgcgaa gtgcgcgtcc ggccggtcct tgagaggacg
     4141 gaacgcgacg cgctcgatga gcaggcccat gaccgcgccg aacgcgaaca cgaggatcag
     4201 caccagccag atcggcagat cgaaggcatc cgtcagccag atgcccacga acgccgaggc
     4261 ggcgaacacg gacggatggg ccagattcag tcgatccagg atgccgaaca ccagggtgaa
     4321 gccgatggcg aacagtgagt agatcgaacc gaggaaaagg ccgttcagca gctgctgaag
     4381 catagggtct cccggcgggt caggggacgg tcaggagcgg atcagtccaa gacggcgaac
     4441 ttgccgtcct tgacgatctg cacgacggcg ttgtgctcgg cgtcgcgatc cttgttgatc
     4501 gtgagcgagc ccagcggcgt cttcagatcc ttgacctcgt tcaggccctt ctggatgtcg
     4561 gcgcgctcgc ccgagcagtt ggcgcggaca gcgtgatcca ggaccagcag gcccgtgtac
     4621 gcctgagcgg cgaactggtc ggggcccgaa ccgtactcgt cctcgtacgc cttcatgaac
     4681 gcggtgttct ccgggctctc cgacgcggag ttccaggcgg cgcccacgac gacaccctcg
     4741 gctgcagggc cggcatcctt catcagctgc gggttgttga agccgttgcc gccgatgatg
     4801 ggaacggtga tgcccagttc gcgcgcctgc gtcaccagcg ggatcgcggc ctcgatgagt
     4861 gccgagacga cgatcgcgtc gggcttcttc tccttggccg cggtcagcag gcttcggaag
     4921 tcggtgtcgc tcttggagaa cttcagggtg tcgacggcgt cgatgccctc cttcttgagc
     4981 gaatccgcca tgaccttgta acccgactcg gtgaaggcgt cgtcgttgct gtacatcacg
     5041 acgaccttct tcagatcgaa cttgtcggcc gcggccttga tcgtctgcgg gatcacctgg
     5101 ccctcggtga gcgagtcgcg gaagatgtag tcgccctggg cggtgatgcc cgcggcggtg
     5161 ttggagatcg cgagcaccgg catgccggcg tcctgggcga tcggctgagc ctggaatgcg
     5221 gtgttcgaca gggtggggcc gatcacggcg ctggtgctgt cggccacgaa cttctcgaag
     5281 acgccgatgg cctgcttcgg gtcggtgccg tcgtcctcga tcttgaggtc gtacttcacg
     5341 cccttcttcg cgttcagatc cttgagcgcg agcttcaggc cgttctgctg cgattcgccg
     5401 tagctcgcag ccgctccggt gaggctgagt gccgcgccga ggggaacggt gccgtcgatg
     5461 gtgcaggagt cgccggagcc gctgcccttg agaccgccgc cggcgtcgcc tcccgtgcca
     5521 ccgccggtgg cgctgcagcc ggcgaggaga aggacggatg ccgagagcag tgcggcgttg
     5581 cggatcatct tgcgcatgtg aacctccctg atggtcgagg cgcactgtgt ggacggccct
     5641 ggtgcagggc cacccgggcg acatggtccg ggtcttccgc gttcacgccg ccgtcagcct
     5701 cgacgatggg cggtgtggtg cgcggatgag tcgatcatac gcacaccgca ccgctggatc
     5761 gggcatggtg acatgtcaca cgtcagtgcc agaacagccc gaggcccgcg ttcagcagcg
     5821 cgagcacgcc gacgctccag aacatcccgg cgggcacggc ctcgccgcgc ttctggcggg
     5881 ccatgccgat gccgatcagc gcgccgatca cgatcagcac aacgagcttg acggtgatct
     5941 tgatgtagaa gcccgctccc ggttcgaaaa ggcccatcgg ccaggcgagc agcagacctg
     6001 cgatgccggc gatcgccagg ccgacgttca tcagctgcgt gaactgcttc ttgccgccga
     6061 acgcctgagt tgcccaggcg ccgaacaggg tggcgaagcc gatgatgtgg aggaagacga
     6121 cgatctcacg gagggttccc atgccgtcca ggctatccag gaacggtccg tcgcggaatg
     6181 cgatacgcga acgggctcag cggaaccgat gccggtccgc tgagcccgtc gagaagggca
     6241 tcagccccgc aggtccgcga tcacgcgggc ggtgcgcaac gcggcatccg cggcctcggc
     6301 gcccttgtcc tcctgggagc ccggcagccc cgcacggtcg aggccctgct gttcgtcgtc
     6361 gagcgtgagc aggccgaaac cgaccggctt gccggcgtcg agcgagacgc gggtgaggcc
     6421 atcggtggtg gcggccgaga cgtactcgaa gtggggcgtt ccgccgcgga tgatgacgcc
     6481 gagggcgacg acagcatccg ctccgccggc gaacgcggcc tgcgccgcga cggccagctc
     6541 gaacgacccg ggcacgcgca ccagggtgtg ctcggctccc gcggcatcca gcgtgcgctg
     6601 cgcgccggcg atgaggccgt tggagatgac ctcgtgccag gtgccggcga tcacgacgac
     6661 ccgcaggccc gtgccgtcga ccggggtgag aggggtgggt gctccggcgc cgctcatgcg
     6721 tcgtcctttc cgtgggcgag ggcctcagcc agctcggact cgccgatgat gtggcccatg
     6781 cgatcgcgct tggtctcgag gtactggtgg ttgttggggc cgacgccgac gatcagcggc
     6841 acctgctcga cgacgtccag gcccagctcg cgcagctgct tcaccttgtc ggtgttgttg
     6901 gtcagcagcc gcaccttgga gacgccgaga tccgagagga tgccggcggc ggccgcgtag
     6961 tcgcgggcgt cggcgggcag gccgagcgcg aggttggcgt cgacggtgtc gaggccctcc
     7021 tcctgcaggc tgtaagcgcg cagcttgttg atcaggccga tgccgcgtcc ctcgtggccg
     7081 cgcatgtaga tgaccacgcc gccctcggcg tcgatgcgat ccagtgccgc ctgcagctgc
     7141 gggccgcact cgcacttctg cgagccgaag gcctcgccgg tcaggcactc ggagtgcacc
     7201 cgcaccaggg cggtctcgcc gggcgtgccc gagacgaccg cgatgtggtc ggtgccggtg
     7261 acacggtcct tgtaggcgag gaaccggaag gtcccgtgct cggtcggcac ggtcgcgtcg
     7321 gcgcgcaggc tgacgcgacg ggcggtcttc ttggcccggc agtcggcctc gtcgcgcggg
     7381 tcgacctcgt tcaggtgccg gatgagctgc tcgatggtga tcaccgggat gtcctcgcgg
     7441 gcgcccagct cctgcagcgc aggcagtcgc atcatgctgc cgtcctcggc gacgacctcg
     7501 gcgatcgcgc cgaccgggtg caggcccgcc aggcgcatca gctccacggc cgcctcggtg
     7561 tgaccgcc
//
LOCUS       DATMSX010000121         7298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1853,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000121 DATMSX010000000
VERSION     DATMSX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /gene="thiD"
                     /locus_tag="VN041_03770"
     CDS             <1..1509
                     /gene="thiD"
                     /locus_tag="VN041_03770"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWU28197.1"
                     /translation="AEFASRWRAAGIPRVLSIAGSDPSGGAGIQADLKSIAANGGYGM
                     AAITALTAQNTQGVWAVHVPPAEFLRDQLDAISDDVVIDAVKVGMLATADVIRVVAGW
                     LDTVRPPIVVIDPVMIATSGDRLLDAEAEQALGDLLRRADVVTPNLSELGVLTGREVT
                     RWEDALAAASVLSAAIDAQVLVKGGHLEGADAPDALVGRGPIVEFPGARIETANTHGT
                     GCSLSSALATRLARGESPAEAVAGSRAWLREAMRASDELHVGRGHGPVNHFAGLWRRG
                     GLETSPTDADIRSDWWRSIEGIRRDIDELPFIRALADGTLDRELFLTYLAQDALYLRE
                     YARVLAEAARLAPTPEEQAFWAESSHGSIAGELELHATWLTPETGISAATFAAEPSPV
                     NTAYLDHLRAAAFSGDYGVLIAAVLPCFWLYTDLGRRLHAGDFGAYATDPRHPYASWL
                     VTYADPAFAIATERAIGYVVAAAAGADGERRRRMHRAFVAAAEHERAFFDLR"
     gene            1791..2861
                     /gene="argC"
                     /locus_tag="VN041_03775"
     CDS             1791..2861
                     /gene="argC"
                     /locus_tag="VN041_03775"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWU28198.1"
                     /translation="MTYSVAVSGASGYAGGEILRLLAAHPDIEIRTVTAHSNAGQPLV
                     QHQPHLRSLAHLTLQDTTPEILAGHDIVFLALPHGQSGQYTDALGEVPLVIDAGADHR
                     LTSQDAWDAFYGGAFHDPWTYGVPELLVDGSKQRENLRGATRIAAPGCNASTVSLSLA
                     PGVAAGVIDPSDIVTVLAVGPSGAGKSLKTHLLGSELLGTANPYAVGGTHRHIPEIRQ
                     ALAAASGPSSGSGTGQEAIRISFTPVIVPMARGILATSTAPIADGATDAQIRDAWESA
                     YGDETFVQLLPEGQFPRTADVLGANTALMGIAIDRAANRVIVVTAVDNLVKGTAGAAV
                     QSMNLALGLPEDRALTVNGVAP"
     gene            2858..4015
                     /gene="argJ"
                     /locus_tag="VN041_03780"
     CDS             2858..4015
                     /gene="argJ"
                     /locus_tag="VN041_03780"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWU28199.1"
                     /translation="MSVTAPQGFEAAGIAVGLKSTGKPDVAVVVNRGPRKVGAAVFTS
                     NRAKANPILWSQQVIADRTVEAIVLNSGGANCFTGAFGFQTSHLTAEKAAELLEVSAG
                     DILVCSTGLIGTGDEVFRGKVLDGVEQGIASLSADGGQLAAEAIMTTDTVSKTAVVTR
                     DGWTIGAMAKGAGMLAPGLATMLVVITTDADLEASEADAALRRATHVSFDRLDSDGCM
                     STNDQVTLLVSGASGIRPDLDDFDAALAELCVELAQKLQGDAEGASHDITIEVVNAAT
                     ETDAVEVGRSVARNNLFKAAIFGNDPNWGRVLAAIGTTRAEFDPYAVDVTMNGIRVCS
                     NGGPDRPRDEVDLAPRATHLLIDLKAGDATATILTNDLTHDYVHENSAYAS"
     gene            4012..4908
                     /gene="argB"
                     /locus_tag="VN041_03785"
     CDS             4012..4908
                     /gene="argB"
                     /locus_tag="VN041_03785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWU28200.1"
                     /translation="MTQLQDTAPEEAAYKAATLIESLPWLKKFRDQIVVVKYGGNAMV
                     SDELQDAFAQDIAYLRYVGVQPVVVHGGGPQISTMLDRLDIPSEFKGGYRVTNTEAIS
                     VVRMVLTGQVNPQLVGKINSHGPIATGVSGEDAGLFGGRRRGVVIDGQEVDLGRVGDV
                     VQVDPTPVLDHLAAGRIPVVSSIAPDLDHPGQSLNVNADAAAAALAVALGARKLVILT
                     DVAGLYADWPNRDSLVSHITAEALTEMLPALESGMIPKMRACLDAVQSGVDAAAIIDG
                     RVPHSVLVELFTSKGIGTEVVA"
     gene            4905..6110
                     /locus_tag="VN041_03790"
     CDS             4905..6110
                     /locus_tag="VN041_03790"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HWU28201.1"
                     /translation="MSDWNEDAAHDLMSLGGRFALLTRGEGSWLWDADEKRYLDFLGG
                     IAVNCLGTAHPVFVEAVSKQAATLAHVSNYFASPGQLELAARLKRLAGTGDAGRVFLA
                     NSGSEANEMAFKLARLHGKPSGRTKMIVVEGAFHGRTMGALSLTPKAMYRDPFLPAVP
                     DVVTVSRSLESLEAAFAAGGVAAMIVEPIQGEAGVVELPEGFLARARELTTEHDSLLI
                     LDEVQTGSGRTGTWFAFQREGIVPDAVTMAKSIGAGFPLSALITFGAASELFYPGTHN
                     STFGGNPLAAAVANATLGFIEETGLLDNVNARGAQLRSAVADLPFVTGTTGAGLLIGI
                     RLSAPVAAEVRAAAHERGLIINAPTEDIIRIAPAYTIGDAEVAEFLTLFSAALDATAI
                     AHSTMETPA"
     gene            6107..7042
                     /gene="argF"
                     /locus_tag="VN041_03795"
     CDS             6107..7042
                     /gene="argF"
                     /locus_tag="VN041_03795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWU28202.1"
                     /translation="MTRHLLRDDDLTPAEQSEVLDLAIELKKDRWSNKALAGPQTVAV
                     IFDKSSTRTRVSFAVGIADLGGSPLIISTANSQLGGKETPSDTARVLERQVAAIVWRT
                     YAQSGLEEMARGTRVPVVNALSDDFHPCQLLADLLTIREHKGELKGLTLSFYGDGRSN
                     MAHSYLLACVTAGMHVRVAAPDAYAPRADVVVAAEKRAAETGGSVTLLTDPREAALGA
                     DIVVTDTWVSMGKEEEKIERLRDLGGYKVTTEIMDLADPEAIFIHCLPADRGYEVDSE
                     VIDGPQSVVWDEAENRLHAQKALLVWLLGKQEDAA"
     gene            7039..>7298
                     /locus_tag="VN041_03800"
     CDS             7039..>7298
                     /locus_tag="VN041_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWU28203.1"
                     /translation="MSSEAHGTNEGALWGARFAGGPSPELAELSRSTHFDWILAPYDI
                     AGSHAHATALEAAGYLEAEEARIMHAGLDALAARIADGTLLDR"
BASE COUNT         1121 a         2675 c         2456 g         1046 t
ORIGIN      
        1 gccgagttcg cgagccgctg gcgcgccgcg ggcattcccc gggtgctcag catcgccggc
       61 agcgatccgt ccggcggcgc gggcatccag gcggacctga agtccatcgc cgcgaacggc
      121 ggctacggca tggcggcgat caccgctctc accgcgcaga acacacaggg ggtgtgggct
      181 gtgcacgtgc cgccggccga gttcctgcgc gaccagctcg acgcgatctc ggacgacgtc
      241 gtcatcgacg cggtgaaggt cgggatgctc gcgaccgcgg acgtcatccg cgtcgtcgcc
      301 ggctggctcg acaccgtgcg tccgccgatc gtggtcatcg accccgtgat gatcgccacg
      361 agcggcgacc ggctgctgga cgccgaggcc gagcaggcgc tgggtgatct gctgcgtcgc
      421 gccgatgtgg tcaccccgaa cctctccgag ctcggcgtgc tgaccggccg ggaggtcacc
      481 cgctgggagg acgcgctcgc cgcggcatcc gtgctctccg ccgcgatcga cgcacaggtg
      541 ctcgtcaagg gcggtcacct cgagggcgct gacgccccgg acgccctcgt cggacgcggt
      601 ccgatcgtcg agttcccggg cgcccgcatc gagaccgcga acacgcatgg caccggatgc
      661 tcgctgtcct cggcgctcgc cacccgtctg gcgcgcggcg aatcgcctgc agaagccgtc
      721 gccggatccc gggcctggct gcgcgaggcg atgcgcgcga gcgacgaact gcacgtcggt
      781 cgcggacacg gccccgtcaa ccatttcgcc gggctgtggc gacgcggcgg cctcgagacg
      841 tcaccgacgg acgcggacat ccgctcggac tggtggcgga gcatcgaggg catccggcgc
      901 gacatcgacg agctgccgtt catccgcgcc ctcgccgacg gaaccctcga tcgcgagctg
      961 ttcctgacct acctcgcgca ggacgccctg tacctgcgcg agtacgcgcg cgtgctggca
     1021 gaggccgccc gcctcgcgcc cacccccgag gagcaggcgt tctgggcgga gtcctcgcac
     1081 ggatcgatcg cgggtgaact cgagctgcac gccacctggc tgacgcccga gacggggatc
     1141 tcggcggcga ccttcgccgc cgagccgagc ccggtgaaca ccgcctacct cgatcacctc
     1201 cgcgccgccg cgttctccgg cgactacggc gtgctgatcg cggcggtcct gccgtgcttc
     1261 tggctgtaca cggatctggg acgccgactg cacgccggcg acttcggcgc atacgcgacc
     1321 gacccgcggc atccgtacgc ctcgtggctg gtgacatacg ccgaccccgc cttcgcgatc
     1381 gccaccgaac gggcgatcgg ctacgtcgtc gctgcggccg cgggtgccga cggggagcgc
     1441 cggcgccgca tgcaccgggc gttcgtcgct gcggccgagc atgagagggc gttcttcgat
     1501 ctgcgctgag cccagcggag cgggcggcag cggagagtgt gactccgcgg atttgcggat
     1561 gcagcgcgat cgacgctact gtcgcgtcat gccttcggcc gcacacctct cgctgacgcc
     1621 ccttccgggc gtcgctcccg tgcgccgacg ccgcgccggc cgccgactct aggcgacccc
     1681 gcgcagcccg ttccgcgatg gttcgaggcc ggagccggtg tcgccgcccc ggtccactgt
     1741 cactgggtca tgcccgtcat cgggcgcgac tcgaccgtta aggtagaacc atgacgtact
     1801 ccgtcgccgt ctccggcgca tcaggctatg caggaggcga gatcctgcgg cttctcgccg
     1861 cgcatcccga catcgagatc cgcaccgtca ccgcgcactc caacgccggt cagccgctcg
     1921 tgcagcacca gccgcacctg cgctcgctcg cgcatctcac gcttcaggac accacccccg
     1981 agatcctcgc cggacacgac atcgtcttcc tcgcgctgcc gcacgggcag tcggggcagt
     2041 acacggatgc tctgggcgag gtcccgctcg tgatcgacgc gggtgccgac caccggctca
     2101 cctcacagga cgcgtgggat gccttctacg gcggcgcctt ccacgacccg tggacctacg
     2161 gggtccccga gctgctcgtg gacggtagca agcagcgcga gaacctgcgt ggcgcgacgc
     2221 gcatcgcggc tcccggctgc aacgcgtcga ccgtgagcct cagcctcgcc cccggagtgg
     2281 cggcgggcgt catcgatccg tccgacatcg tgactgtcct cgccgtgggg ccgtccggtg
     2341 caggcaagag cctgaagacg cacctgctcg gcagcgagct gctcggcact gccaacccct
     2401 atgcggtcgg cggaacgcac cggcacatcc ccgagatccg ccaggcgctc gccgcggcct
     2461 ccggtccttc gtcgggctca ggaaccggtc aagaggcgat ccgcatctcc ttcaccccgg
     2521 tgatcgtccc gatggcccgc ggcatcctcg ccacctcgac ggcaccgatc gccgacggcg
     2581 cgaccgacgc gcagatccgc gacgcctggg agtccgcgta cggcgacgag accttcgtgc
     2641 agctgctgcc cgaaggccag ttcccgcgca ccgccgatgt gctgggtgcc aacaccgcgc
     2701 tgatgggcat cgccatcgac cgtgcggcga accgcgtgat cgtcgtcacc gcggtcgaca
     2761 acctcgtcaa gggaacggcc ggcgccgccg tgcagtccat gaaccttgcg ctgggccttc
     2821 ccgaggaccg cgccctcacc gtgaacggag tagcgccgtg agcgtcaccg caccccaggg
     2881 attcgaagca gccggcattg ccgtcggcct gaagagcacc ggcaagccgg acgtggccgt
     2941 ggtcgtcaac cgcgggcccc gcaaggtggg agccgcggtc ttcaccagca accgcgccaa
     3001 ggccaacccc atcctgtggt cgcagcaggt catcgccgac cgcacggtcg aggcgatcgt
     3061 gctgaactcc ggtggcgcga actgcttcac cggcgcgttc gggttccaga ccagccacct
     3121 caccgccgag aaggccgccg agctgctcga ggtgagcgcc ggagacatcc tggtctgttc
     3181 cacgggcctc atcggcaccg gcgacgaggt gttccgcggc aaagtgctcg acggcgtcga
     3241 gcagggcatc gcctcgttgt ccgccgacgg cggccagctc gccgcagagg ccatcatgac
     3301 caccgacacg gtctcgaaga ccgccgtcgt cactcgcgac ggctggacga tcggagcgat
     3361 ggcgaagggt gcgggcatgc tcgctccggg cctggccacc atgctcgtcg tcatcaccac
     3421 ggatgccgat ctcgaggcgt ccgaggccga tgctgcgctc cgccgggcca cgcacgtcag
     3481 cttcgaccga ctcgactcgg acggctgcat gtcgaccaac gaccaggtga ccctgctcgt
     3541 gagcggagcg agcggcatcc gtcccgacct cgacgacttc gacgccgcgc tcgccgagct
     3601 gtgcgttgag ctcgcgcaga agctgcaggg cgacgccgag ggcgccagcc atgacatcac
     3661 gatcgaggtc gtgaacgccg ccaccgagac cgacgcggtc gaggtcggcc gctcggtcgc
     3721 gcgcaacaac ctgttcaagg cggcgatctt cggcaacgac ccgaactggg gccgcgtgct
     3781 cgccgcgatc ggcaccaccc gcgcggaatt cgacccctac gccgtggacg tcacgatgaa
     3841 cggaatccgc gtctgctcga acggcggacc ggaccgtccg cgcgacgagg tcgacctggc
     3901 cccgcgcgcc acgcacctgc tgatcgacct gaaggcgggc gacgccacgg ccacgatcct
     3961 caccaacgac ctgacccacg actacgtgca cgagaacagc gcctacgcct catgacccag
     4021 ctccaggaca ccgcacccga ggaggccgcc tacaaggccg ccaccctcat cgagtcactg
     4081 ccgtggctga agaagttccg cgatcagatc gtcgtggtca agtacggcgg caacgccatg
     4141 gtcagcgatg agctgcagga cgctttcgcg caggacatcg cctacctgcg ttacgtgggc
     4201 gtgcagcccg tggtggtgca cggcggcggt ccccagatct cgacgatgct ggaccggctc
     4261 gacatcccca gcgagttcaa gggtggatac cgcgtcacca acacggaggc gatcagcgtc
     4321 gtgcggatgg tgctcaccgg gcaggtcaac ccgcagctgg tcggcaagat caactcgcac
     4381 ggtcccatcg ccacgggcgt gagcggcgag gatgccggcc tgttcggcgg acgccgtcgc
     4441 ggtgtggtca tcgacggcca ggaggtggat ctcggtcgcg tcggcgacgt ggtgcaggtc
     4501 gatccgacgc cggtgctgga ccatctcgca gccggccgga tccccgtggt ctcctcgatc
     4561 gcgcccgatc tggatcaccc cggacagtcg ctgaacgtga acgcggatgc cgctgcggcg
     4621 gcgctggccg tggccctcgg cgcccgcaag ctggtcatcc tgacggatgt cgccggcctg
     4681 tacgccgact ggccgaaccg cgactcgctc gtctcgcaca tcaccgccga ggcgctcacc
     4741 gagatgctcc cggcgctgga gtccgggatg atcccgaaga tgcgcgcctg cctggacgcc
     4801 gtgcagagcg gcgtggacgc cgccgccatc atcgacggac gggtgccgca ctcggtgctc
     4861 gtcgaacttt tcaccagcaa gggcatcggc acggaggtcg tcgcatgagc gactggaacg
     4921 aggacgcagc acacgatctg atgagcctgg gcgggcggtt cgcgctgctc acgcgcggcg
     4981 aggggtcctg gctgtgggat gccgatgaga agcgctacct cgacttcctc ggcggcatcg
     5041 cggtgaactg cctgggcacg gcccacccgg tgttcgtcga ggcggtctcg aagcaggccg
     5101 cgacgctcgc gcacgtgtcg aactacttcg ccagccccgg gcagctcgag ctcgccgcac
     5161 gactcaagcg tctggcggga accggcgacg cgggccgggt gttcctcgcg aactccggca
     5221 gcgaggccaa cgagatggcc ttcaagctgg cccgtctgca cggcaagccc tcgggccgca
     5281 cgaagatgat cgtcgtcgag ggggcgttcc acggtcgcac catgggtgcg ctgtcgctca
     5341 cgcccaaggc gatgtaccgc gatcccttcc tgcccgccgt ccccgatgtg gtgaccgtgt
     5401 cgcgctcgct cgagagcctc gaggcggcgt tcgcagccgg gggagtggcg gccatgatcg
     5461 tcgagcccat ccagggcgag gcgggcgtcg tcgagctccc cgagggcttc ctcgcccgtg
     5521 ccagggaact gacgactgag cacgactcgc tgctcatcct cgacgaggtg cagacaggtt
     5581 ccgggcgcac cggcacgtgg ttcgcgttcc agcgcgaggg catcgtcccc gacgccgtga
     5641 cgatggccaa gagcatcggc gcaggctttc cgctgagcgc cctcatcacg ttcggcgcgg
     5701 ccagcgagct gttctacccc ggcacccaca actcgacgtt cggcggcaac ccgctggccg
     5761 ccgcggtggc caacgccacg ctgggcttta tcgaggagac cggtcttctc gacaacgtca
     5821 acgcgcgcgg cgcgcagctg cgttcggcgg tcgcggacct cccgttcgtg accggcacca
     5881 ccggggcggg gctgctcatc ggcatccgcc tctccgcgcc cgtcgccgcc gaggtgcgag
     5941 cggccgccca cgaacgcggc ctgatcatca acgcacccac cgaggacatc atccgcatcg
     6001 cacccgcgta cacgatcggc gacgccgagg tggcggagtt cctcaccctc ttcagcgcgg
     6061 cgctggacgc cacagcaatc gcacactcga ccatggagac ccccgcatga cccgccacct
     6121 gcttcgcgat gacgacctga cccccgccga gcagagcgag gtgctggatc tcgcgatcga
     6181 actgaagaag gatcgctggt ccaacaaggc cctcgccggc ccgcagaccg tcgccgtgat
     6241 cttcgacaag tcctccaccc gcacccgggt gtcgttcgcc gtggggatcg cggacctcgg
     6301 tggctcgccg ctgatcatct ccaccgccaa cagccagctc ggcggcaagg agacgccgtc
     6361 cgacacggct cgcgtgctgg agcggcaggt ggcggcgatc gtgtggcgca cctacgcgca
     6421 gtccggtctc gaggagatgg cgcgcggcac ccgcgtgccg gtcgtcaacg cgctctcgga
     6481 cgacttccac ccctgccagc tcctggccga cctgctcacg atccgcgagc acaagggcga
     6541 gctcaaggga ctcacgctgt cgttctacgg tgacggccgc agcaacatgg cgcactccta
     6601 cctcctggcc tgcgtcaccg ccggaatgca cgtgcgcgtg gccgcgccgg atgcgtacgc
     6661 gccccgcgcg gacgtggtcg tcgccgccga gaagcgcgct gccgagacgg gcggatccgt
     6721 caccctgctc accgacccgc gcgaggcggc gctcggcgcc gacatcgtgg tgaccgacac
     6781 ctgggtgtcg atgggcaagg aggaggagaa gatcgagcgg ctccgcgatc tgggcggcta
     6841 caaggtcacc accgagatca tggacctcgc cgatcccgag gccatcttca tccactgcct
     6901 ccccgccgat cgcggctacg aggtggactc cgaggtcatc gacgggccgc agagcgtggt
     6961 ctgggacgag gccgagaacc gcctgcacgc gcagaaggcg ctgctggtct ggctcctggg
     7021 caagcaggag gacgccgcat gagctccgag gcgcacggca cgaacgaggg cgctctctgg
     7081 ggtgcacggt tcgcgggcgg gccgtcgccc gagctcgccg agctgagccg ctccacgcac
     7141 ttcgactgga tcctcgcccc gtacgacatc gcggggtccc acgcacacgc cacggctctc
     7201 gaggccgcgg ggtacctgga ggcggaggag gcgcgcatca tgcacgccgg cctcgacgcc
     7261 ctcgccgcgc ggatcgcgga cggcacgctg ctagatcg
//
LOCUS       DATMSX010000122         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20417,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000122 DATMSX010000000
VERSION     DATMSX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20417"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN041_03805"
     CDS             complement(<1..219)
                     /locus_tag="VN041_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWU28204.1"
                     /translation="MIIAHDLGTTGDKASLHHDDGRLVASVTVPYPAHFAAGGIAEQD
                     PQDWWDAVVGATRELIARTGTSPAEVAGL"
     gene            303..899
                     /locus_tag="VN041_03810"
     CDS             303..899
                     /locus_tag="VN041_03810"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HWU28205.1"
                     /translation="MPALDIPVTLTISPEGGIEGRSRRYEKHLGDMSGVYRDEAAWEA
                     SVAERGADELVYWVDDHRYQEGPGALITGTSTLLPGRVGEEFAVTRGHLHAIADRAEL
                     YYCLSGRGVMLLETVDGRSEAIELTPGKAVNVPGHWIHRSVNVGDEPFVTLFCYAADA
                     GQDYALIADAGGMKNLVTVDGDGWALTPNPDHTGYRRA"
     gene            983..1342
                     /locus_tag="VN041_03815"
     CDS             983..1342
                     /locus_tag="VN041_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU28206.1"
                     /translation="MAHGDITHIDIPVSDLTAASAFYSKLFGWNIAEMPGFEGYPMWQ
                     APNGISGGGLAPRDDGFTQPRSYVEVDSIDDTIAAAKEAGATVAAEKQPITETSAWAV
                     IVDPDGNHIGLFEGVVG"
     gene            complement(1361..>1642)
                     /locus_tag="VN041_03820"
     CDS             complement(1361..>1642)
                     /locus_tag="VN041_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylesterase"
                     /protein_id="HWU28207.1"
                     /translation="RHPEAREQLRPFDASTAALSFRIPVRVEAALWDQNVPPQGQFAV
                     ANAVPGELLELHVLPAGHAEYPGLESITADAVAGGLAHLDRALAAAARR"
BASE COUNT          260 a          575 c          579 g          228 t
ORIGIN      
        1 caggccggcg acctcggcag gagacgtgcc cgtccgcgcg atcagctcgc gcgtggcacc
       61 gaccacggcg tcccaccagt cctgcgggtc ctgctcggcg atgccgccgg cggcgaagtg
      121 ggccgggtac ggcacggtga cggatgccac gagccggccg tcgtcgtggt gcagcgaggc
      181 cttgtcgccg gtggtgccga ggtcgtgcgc gatgatcaca gtccgtgcct gtctttgtgc
      241 ggtctcaggt gtttggagta tacctgtaat tacaagttac caggaggccc atcgtgagca
      301 ccatgcccgc cctcgacatc cccgtcaccc tgaccatctc gcccgagggc ggaatcgaag
      361 gacgctcgcg tcgctacgag aagcacctcg gcgacatgtc gggtgtgtac cgcgacgagg
      421 ccgcctggga ggcgtccgtc gccgaacgcg gcgccgacga gctggtctac tgggtcgacg
      481 accaccgcta ccaggagggc cccggcgctc tcatcaccgg gacgagcacc ctgctgcccg
      541 gccgcgtggg cgaggagttc gcggtgaccc gaggacacct gcacgcgatc gccgatcgcg
      601 ccgagctgta ctactgcctg agcggccgcg gcgtgatgct gctggagacc gtcgacggcc
      661 gttccgaggc gatcgagctg acccccggca aggccgtgaa cgtgccgggg cactggatcc
      721 accgcagcgt gaacgtgggc gacgagccct tcgtcaccct gttctgctac gccgcggacg
      781 ccggccagga ctacgcgctg atcgcggatg ccggtggcat gaagaacctc gtcacggtcg
      841 acggcgacgg ctgggcgctg acccccaacc ccgaccacac cgggtaccgc cgcgcctgat
      901 cggatgaccc tcttgtcctg cgcctccggg aggcgcagac tgtccccgtc atggcgacga
      961 gaactgacga gaggatcgga tcatggcgca cggagacatc acccacatcg acatcccggt
     1021 gtccgacctg acggccgcga gcgcgttcta cagcaagctc ttcggctgga acatcgccga
     1081 gatgccgggc ttcgagggct acccgatgtg gcaggccccg aacggcatct ccgggggcgg
     1141 gctcgcgccc cgcgacgacg gcttcacgca gccgcgatcg tacgtcgagg tcgactccat
     1201 cgatgacacg atcgccgcgg cgaaggaggc cggggcgacc gtcgccgccg agaagcagcc
     1261 catcaccgag accagtgcct gggccgtgat cgtcgacccc gacggcaacc acatcgggct
     1321 cttcgagggc gtcgtcggct gacgaggccc gggcgaggcg tcagcggcgc gcggctgcgg
     1381 ccagcgcccg gtccaggtgc gcgaggccac cggccacggc atccgcggtg atcgactcga
     1441 gaccagggta ttcagcgtgc ccggcgggga ggacgtgcag ctcgagcagt tcccccggaa
     1501 cggcgttggc gacggcgaac tggccctgcg gcggcacgtt ctgatcccac agcgcggcct
     1561 cgacgcgcac ggggatgcgg aaggacagcg ccgcggtcga cgcgtcgaac gggcgcagct
     1621 gctcgcgcgc ctccggatgc cg
//
LOCUS       DATMSX010000123        15958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_153424,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000123 DATMSX010000000
VERSION     DATMSX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 15958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15958
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153424"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1845)
                     /locus_tag="VN041_03825"
     CDS             complement(409..1845)
                     /locus_tag="VN041_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWU28208.1"
                     /translation="MDEIANTAEGPVRGIRRADGSHAFLGIPYAAPPTGKRRFQPPQP
                     VTPWTEPLDATAYGPTPQRRPEDNAIIPEPSVPGTATLNLNVFTPALHGSAPVLVWIH
                     GGGYSAGSSASPWYDGASFTRDGVVLVTISYRLGFDGFGVIDGVPDNRGVRDWIAALE
                     WVQRNIASFGGDPGRVTIAGQSAGGGAVLTLFGIPAAQHLFHAGISISGALGDLPRER
                     ARARSELLASMVGCEPTLEGFRGVKERALTRRQFEASLLGKKGLAATTATLTDGLPWG
                     PVIDGDLITRPTVDSFAAGNGAGKPLMLGATDDEFTMIFDNAPRALRWVPVPFALRVV
                     EKTGVLRRRWRRSNRARRGAGPALGRFVTDRIFRSLVVRVAETRREAGTWVYRFAWSS
                     PVNGWSHHCLDVPFWFDCLHEEHVAKMTGAHPPQSLASEMHSAAVAFVTSGDPGWPAW
                     RSEPGITRIFGDQPALSRSAYDDALPLV"
     gene            complement(1878..5042)
                     /locus_tag="VN041_03830"
     CDS             complement(1878..5042)
                     /locus_tag="VN041_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HWU28209.1"
                     /translation="MGIQHEEAFEREICEHLAANGWIYEPENTHGYDKTRALFPEDVF
                     AWIQQTQPGEWAKVVKPTASVAEQGKAKHQLLDRLVKTLDLPLDSGGGVLNVLRRGFK
                     KTPATFRMCAFKPATTRNAKAMQDYAAVRLRVVRQVYYSAKNSNSIDLVLFANGIPVA
                     TLELKTDNMQKLDDAIAQYRHDRDPKGEPLLGFANRALVHFAVSTDEVAMTTRLAGAA
                     TRFLPFNLGRDQGAGNPVNPDGAASSYLWERVLQRDAWLNILGRLLHIEKSQSTDPIT
                     GEVTKHTTLLFPRFHQWELVTELLQTAREEGPGNRYLVQHSAGSGKTNSIAWLAHGLS
                     SLHDDANHKVFDSVIVITDRTVLDDQLQKAIKQIEGVDGTVATINADEVRKAGEGSKS
                     GLLAKTLLSGKHIIIVTLQTFPYALKAIQENAGLSGRSFAIIADEAHSSQTGEASKKL
                     REVLTTDEQAALDDGGEVDAEALLAAQMTARADSPNLSFFAFTATPKPKTLELFGRPD
                     ADGIPRSFHVYSMRQAIEEGYILDVLRNYTTYSTAFQLAEKTRRHLQIVDEVDEGEAV
                     KGLMRWVSLHPTNIAQKVQIIVEHYRANVRHLLDGHAKAMVVTASREHAIRYKEAIDA
                     YIASKKYPLATLVAFSGTMTADQVPGVAFKGVEPPYSESNLNAGLRGRSIPTAFASDD
                     FQILIVANKYQTGFDQPLLCAMYVDKRLDGVTAVQTLSRLNRTYPAGGKDTTYILDFV
                     NDPQEILATFRQYHRTAELSETSDPDQVHDLQGKLDAAGIYTDADVEAFANAYLATAT
                     PPKHGAHTAPLKAAADRFNTRYDAATAFGSKAERDELDMFRKDVGTFIRFYDFLSQIF
                     DYADTGLEKRSLYLRLLLPRLTGRVGGEPIDFTSVELTHVKQTRHGDDVLDLDAGTAD
                     AMHPAGAGGTGTARDPKLARLADILARINELFADEDFSQSGVQSWAEGVVTVLTENPQ
                     LRTQASANTRKQFVESPDLNDAVTDAVLSNQDTTNRLVQYYLAHPEMRGKLVQLLGAL
                     VHENVHGEDDRGADARGADEAKPA"
     gene            5106..5528
                     /locus_tag="VN041_03835"
     CDS             5106..5528
                     /locus_tag="VN041_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28210.1"
                     /translation="MSSTPLSIRFDDAVLDRVRRSASARDVTASSLIQRYVEEGLRTQ
                     DFPGIVFRDGPSGRRAALAVGPDVWEIASALRDAGARGDAAVRAVAVDFALPESAVRV
                     ALAYYGAYSAEIDSEVAENDRAAEEALRSWQAQRRLVS"
     gene            5525..5878
                     /locus_tag="VN041_03840"
     CDS             5525..5878
                     /locus_tag="VN041_03840"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWU28211.1"
                     /translation="MTSLLLLDEQYSDVIAVALRGRGHDVVSVVADAGLRGVSDAELF
                     HWAASAGRRIVTENIKDFRPLLVAALASGAPIAPLLLVPPRRFPRSVDAIIAALGAWL
                     ENTEPHVRPVEDWLV"
     gene            5983..7395
                     /locus_tag="VN041_03845"
     CDS             5983..7395
                     /locus_tag="VN041_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWU28212.1"
                     /translation="MNSTAAVLDRVASDLDLVLGADALSTLTDSERLSVLNAAGEVLR
                     RVEAVVVETVATSDPADAPNSAGCRSMNELLQRTLRVDSRTAAQMAKAGRLTHRDTDL
                     TSGESLPAKWPALRQALLDGDIGVTGLLAATGPIEAAGDRISAADRWKADALLADVAR
                     GYAMSDDDGVGQDAAPAPTPEDLKALAHEIAVRLDPDGAEPIEERAMRKRYLTVGRMR
                     DGVYPMRGNLLPETYAQLQLLLDAQLNPKTEGPPVPEGVAFRPSAGETDAVEDEFNSD
                     PRSVLDTRTRGQKQHDALAAALGIAARHSEMPSLGGAAPTLVVHIDFDDDSWARIPGV
                     DGPVSGAVARHTGCTGALQRVFTEKGRIVGMTITDRIFDVYQRRAITLRDKECLIPGC
                     HVPASWCEIHHVIEHARGGPTSTDNGVPLCWWHHRSLDHSGWEIRMNDGLPEIRGPSW
                     WDPLQRWRVPRLSLPQFAFT"
     gene            complement(7572..8846)
                     /locus_tag="VN041_03850"
     CDS             complement(7572..8846)
                     /locus_tag="VN041_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWU28213.1"
                     /translation="MSSGHGIAWAPDLPEHWTASHLRWITSIYAGGTPDRNNADYWRD
                     GTVPWLNSGSVNDWSITKPSEFISEAGLSGSSARWVPPRSVVVGLAGQGKTKGTAARL
                     EIPATTNQSMAAIIPGGTLEYRYLHYWLVSNYQSIRNLAGGDKRDGLNLQHIAGIEIP
                     LPPFDEQLAIADYLDRETARIDTLIAKQEQLIATLRERRSATVTQLSLEGLDPTVETA
                     QHPFLGVKNASWSTARFGRVISISGGLVDPRTERWRDSILFAPNHIESGTGRIIGRET
                     AMEQGADSGKYVVKAGQILYSKIRPALNKVAVASEDGLCSADMYALSAASSFDRDYLA
                     YYLLSNPFLAFATMTSMRVKMPKVNREELGEAVIPLPPLPEQKEIADAITAATTKIDT
                     LIAKAQRFIELSKERRAALITAAVTGQLEIPA"
     gene            complement(8843..10582)
                     /locus_tag="VN041_03855"
     CDS             complement(8843..10582)
                     /locus_tag="VN041_03855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HWU28214.1"
                     /translation="MSSLGSFVWQIADQLRGPFKPHQYGDVILPMTILRRLDGVMSAH
                     REAIDEVLLSTSNEDLRDVRIRQKTGLGFYNSSPWTFTRLLGDPQGLQSNLIDYISGF
                     SKAIDVFERFKFEDQIEALAESNRLLLVVQRFAEVDLSPDAMPNAAMGDLFEELIRKF
                     AEASNETAGEHFTPRDAIRLIVDLLFAGDDSGLTEKGAVRSIYDPTAGTGGMLSVAEE
                     RLLEMNPDAKLRLYGQEINPQSYAICKSDMIAKGQDATSIRLGNTLSDDLFAGQTFDY
                     CLSNPPYGVDWKASQAAVKAHIQETGERSRFPGGLPPISDGQMLFLQHIATKMRPVHE
                     GGGRAGIVLNGSPLFTGAAESGPSQIRRWLLEHDLVDAIVALPTNMFYNTGIATYVWL
                     LDNNKPADRIGTVQLIDGTGYWSKLRKNLGQKNREVAKQDRAEIARLYAEHVEQKHSK
                     IFPTDAFGYWTITVERPLRDEDGEIVRDRKGNPKPDSKLRDTENVPFTWGGNEAGADA
                     RDAVIEAYFAAEVLPHVPDAWVDTTKTKVGYEIPFTRHFYEYVPPRPLEEIDAELNQL
                     VGEIMELLREVER"
     gene            10925..11050
                     /locus_tag="VN041_03860"
     CDS             10925..11050
                     /locus_tag="VN041_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28215.1"
                     /translation="MEQKPEIFVEGLSAADLPADIKKPYSEADFRRELRRREGGA"
     gene            complement(11097..11894)
                     /locus_tag="VN041_03865"
     CDS             complement(11097..11894)
                     /locus_tag="VN041_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28216.1"
                     /translation="MAVEPGCPKEIKLALGRCPNTTLTDDTAQVGGTNTKDGSHNHSS
                     GQAKPEKSSKGRAPDSQSYKETEAYKKCAAIFGGADSVCIPRGSYSVVTPAAPDDVDI
                     PPITITDLASFSPTPGTLTGEPDNLGVAGLPTNFTTDAAAHTRTGTLFGYPVTARFTP
                     ASFVFRYGDGQTRTATTAGKTWEALRVPQFTPTDTSHTYAERGTYQASVDTVYTAEID
                     LGTGYFPISGTLTIPGPPQSIQIYEARTALVAHTCDEDPTAEGCPTS"
     gene            complement(11954..12505)
                     /locus_tag="VN041_03870"
     CDS             complement(11954..12505)
                     /locus_tag="VN041_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28217.1"
                     /translation="MTHATSTVRALGALSAGLLALGMLSACTPTPEPDPKPTKTALFA
                     TDEEAFKAAEETYRAYNDAGNARRNGAENPDPQDFLAGSALAADIRGRQDLEAAGLKT
                     IGTAKVSKFVPIIGSVELHGERLAAIVCLDASDITTINAADEDITPIDRPTVLAQKVE
                     MRWIDTGYVITAELDEDDAQCAN"
     gene            complement(12831..14216)
                     /locus_tag="VN041_03875"
     CDS             complement(12831..14216)
                     /locus_tag="VN041_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWU28218.1"
                     /translation="MAQFTDRHGLTHPLPAPEEEALVRADDRAHVFHSWSAQALIDPM
                     PVAAGEGSTFWDYQGNAYLDFSSQLVNLNLGHQHPDLIAAIQQQAGRLATVQPALAND
                     VRGELARLISEVAGDGFEKVFFTNGGADANEYAVRMARQTTGRRKVLSMYRSYHGSTS
                     TAISLTGDPRRWANEVSDAGAVRFFGPYAYRSAFRSESEEQEGERALAHLEQTVQLEG
                     PSTIAAIIIESVVGTNGVLAPPPGYLQGVRELCDRYGIVYIADEVMVGFGRVGEWFGF
                     QAYGGQPDLITFAKGVNSGYVPLGGVVISDRIAAHFDEVSFPGGLTYSGHPLACAVGV
                     ATFEVFRRDGILERVRDLGERIVEPTLRGWAETHPSVGEVRGSGLFWAVELVRNRETR
                     EPLVPFNASGADAAPMAAFAAAAKKAGVWPFVHFNRTHIAPPLVITEEELVRGLSAID
                     EALTVADGYTA"
     gene            complement(14230..15714)
                     /locus_tag="VN041_03880"
     CDS             complement(14230..15714)
                     /locus_tag="VN041_03880"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWU28219.1"
                     /translation="MSTIRHFIAGTYAGTVDRTLPVYDPATGETHRQVAAASTAEVEH
                     AIAVAKEAQRAWRSSSLVKRADVFFRLRQILTERADELAAIVTSEHGKVLSDAAGEVS
                     RGIENVEFAAGLLHHLKGERAEQVSRGVDVHSVKQPVGVVACITPFNFPVMVPLWMVA
                     SAIACGNAVVLKPSEKDPSAAIWLARAFQEAGLPDGVLNVVNGDKVAVDALLDSKDIG
                     AVSFVGSTPIARSIYERASANGKRVQALGGAKNHMVVMPDADIDGAVTAAISAAYGSA
                     GERCMAVSVLVAVGDIADDLVEGIRSRIGELRIGPGTDPASEMGPLITREHRDKVASY
                     VAGAAAEGGTVVVDGTQRAFEGDGFFTGVSLIDHVTTEMKVYQDEIFGPVLSVVRVNS
                     YEEAVELINANRFGNGTAVFTRDGGTARQFEYDIEVGMVGINVPIPVPVGAFSFGGWK
                     DSLFGDSHIYGPESIGFYTRSKVVTTRWPDHTPAQLDLGFPSNH"
BASE COUNT         2429 a         5038 c         5646 g         2845 t
ORIGIN      
        1 gcggccgtga acggctcgcc gagcctggcg atctacgcga acccggtggt cgccgccggt
       61 cgcgtggagc tgctcacctt cctgcgcgag caggccgtgt cggtcaccgc gcaccgcttc
      121 ggcacgccgc accgctacga gatcccgctg ctcgagccgg tgcgctgagc cgcgacaagc
      181 ccgtcgccct gtcgcaggcc cctggtgctc tgggtcctgc ggcagggcgt cgtcgtatcc
      241 gcgtgccggt gccaggcggg catccgagca ggacggaacc tgtgtcgcag tagtggtggg
      301 tacggatgct gcgcaccgtc cgcttatggg acacggtgcc gcggggtgtc gcaggaggaa
      361 tgggtacgga tgcggtgcac catccacttc tgggacagga cgccgcggtt agaccagggg
      421 cagggcgtcg tcgtaggcgc tgcgtgagag agcgggctga tcgccgaaga tccgcgtgat
      481 gcccggctcg ctgcgccagg ccggccagcc gggatcaccg gaggtcacga atgcgacggc
      541 cgccgaatgc atctcggatg ccagcgactg cggcgggtgc gcgcccgtca tcttcgcgac
      601 gtgctcctcg tggaggcagt cgaaccagaa cggcacgtcg aggcagtggt gcgaccaccc
      661 gttcaccgga gagctccagg cgaagcggta gacccaggtg ccggcctccc gccgcgtctc
      721 cgcgacacgc acgaccagcg agcggaagat gcgatcggtg acgaagcgcc ccagagcggg
      781 ccccgcgccg cgacgcgcac ggttggaccg ccgccaccgg cggcgcagca cgccggtctt
      841 ctccacgacg cgcagcgcga acgggacggg cacccatcgg agggcgcgcg gggcgttgtc
      901 gaagatcatc gtgaactcgt cgtcggtggc gcccagcatc agcggcttgc cggcgccgtt
      961 ccccgccgcg aacgagtcga ccgtcggacg tgtgatgagg tcgccgtcga tcacgggtcc
     1021 ccagggaagg ccgtcggtga gcgtggcggt ggtggcggcc agtcccttct tgccgagcag
     1081 ggatgcctcg aactggcgcc gggtgagcgc gcgctccttc acgccgcgga agccctccag
     1141 ggtcggctcg catccgacca tcgaggcgag cagttcgcta cgcgcccgcg cgcgctcgcg
     1201 gggcaggtcc ccgagggctc cgctgatcga gatcccggca tggaacaggt gctgagcggc
     1261 agggatcccg aagagcgtga gcacggctcc gccgccggcg gactggcccg cgatcgtcac
     1321 gcggcccggg tcgccgccga aggacgcgat gtttcgctgc acccactcga gggccgcgat
     1381 ccagtcgcgc acgccacgat tgtcgggcac gccgtcgatc acgccgaacc cgtcgaagcc
     1441 gagccggtac gagatggtca ccagcaccac gccgtcccgg gtgaacgatg cgccgtcgta
     1501 ccaggggctc gcggaggatc ccgccgagta gccgccgccg tggatccaca cgagcacggg
     1561 ggctgagccg tggagggcgg gcgtgaacac gttcaggttc agcgtcgccg tgccgggaac
     1621 gctcggttcg gggatgatcg cgttgtcctc cgggcggcgc tggggcgtcg gtccgtaggc
     1681 cgtcgcatcg agcggctccg tccagggcgt cacgggctgc ggtggctgga agcgccgctt
     1741 tcccgtcggc ggcgcggcgt acgggatgcc caggaacgcg tgtgatccgt cggcgcgacg
     1801 gattccgcgg acgggcccct cggcggtgtt cgcgatctcg tccatcgggt tcaggctacc
     1861 gtcggatccc ttcggtgtca ggccggcttc gcctcatccg cgccgcgggc gtccgcgccg
     1921 cggtcgtcct cgccatggac gttctcgtgc accagcgcgc cgagcagctg gacgagcttg
     1981 ccgcgcatct cggggtgcgc gaggtagtac tgcacgagcc ggttggtggt gtcctggttg
     2041 ctgagcacag catccgtcac ggcgtcgttc aggtcggggg attccacgaa ctgcttccgg
     2101 gtgttcgctg aggcctgcgt gcgcagctgc gggttctcgg tcagcacggt cacgacgccc
     2161 tccgcccagc tctgcacgcc ggactggctg aagtcctcgt ccgcgaagag ctcgttgatg
     2221 cgggcgagga tgtccgccag gcgcgccagc ttcgggtcac gggcggtgcc ggttccgccg
     2281 gcgccggccg gatgcatcgc gtccgcagtc cctgcatcca gatccaggac atcgtcaccg
     2341 tgacgggtct gcttgacgtg cgtcagttcg acggacgtga agtcgatcgg ttcgccgccg
     2401 acgcgtccgg tgagccgagg aagcagcagg cgcaggtaca gcgagcgctt ctccaggccg
     2461 gtgtcggcgt agtcgaagat ctgcgacagg aagtcgtaga agcggatgaa cgtgcccacg
     2521 tccttgcgga acatgtccag ctcgtcccgt tccgccttcg acccgaatgc cgtcgccgcg
     2581 tcgtaccgcg tgttgaagcg gtcggcggcg gccttcagcg gtgcggtgtg cgcaccgtgc
     2641 ttcggcggtg tcgccgtggc gaggtaggcg ttcgcgaagg cctcgacatc ggcatccgtg
     2701 tagatgcccg cggcgtcgag cttcccctgc aggtcgtgaa cctggtcggg atcgctcgtc
     2761 tcgctgagtt cggccgtgcg gtgatactgg cggaacgtcg ccaggatctc ctgggggtcg
     2821 ttgacgaagt cgaggatgta cgtggtgtcc ttgccgcccg cggggtaggt gcggttcagc
     2881 cgtgacagcg tctgcacggc ggtgacgccg tcgagtcgct tgtcgacgta catcgcgcac
     2941 agcaggggct ggtcgaatcc ggtctgatac ttgttagcga cgatgaggat ctgaaagtcg
     3001 tccgaggcga aggcggtcgg gatgctgcgc ccacgcagcc ccgcgttgag gttggactcc
     3061 gagtacggcg gctcgacgcc cttgaaggcg acgcccggca cctggtcggc agtcatggtc
     3121 ccggagaacg cgaccagcgt ggcgagcggg tacttcttcg acgcgatgta ggcgtcgatc
     3181 gcctccttgt agcggatcgc atgctcgcgc gacgccgtga cgaccatcgc cttggcatgt
     3241 ccgtcgagca gatgccgcac gttggcgcgg tagtgctcga cgatgatctg caccttctga
     3301 gcgatgttcg tcggatgcag cgacacccag cgcatgaggc ctttgacggc ctcgccctcg
     3361 tcgacctcgt cgacgatctg caggtgacgt cgggtcttct ccgcgagctg gaacgcggtc
     3421 gagtacgtcg tgtagttgcg caggacgtcg aggatgtagc cctcttcgat ggcctgtcgc
     3481 atggagtaga cgtggaaaga gcgcgggatg ccatcggcat ccggcctgcc gaacagctcc
     3541 agggtcttcg gcttcggagt cgcggtgaac gcgaagaagg agaggttcgg agagtcggcg
     3601 cgtgcggtca tctgcgcggc gaggagcgcc tcggcatcca cctcaccacc gtcgtcgagc
     3661 gcggcctgct cgtcggtggt gagcacttcg cgcagcttct tcgatgcttc gccggtctgc
     3721 gaggagtgcg cctcgtcggc gatgattgcg aaggagcggc cggacaggcc ggcattctcc
     3781 tggatcgcct tcagcgcgta ggggaacgtc tgcagggtga cgatgatgat gtgcttgccg
     3841 gagaggaggg tcttggcgag cagaccggac ttcgatcctt ctccggcttt gcggacctcg
     3901 tcggcgttga tcgtggcgac tgtgccgtcg acgccctcga tctgcttgat cgccttctgc
     3961 agctgatcgt cgagcacggt gcggtcggtg atgacgatca cggagtcgaa gaccttgtgg
     4021 ttcgcgtcgt cgtgcaggga ggacaggccg tgcgcgagcc aggctatcga gttggtcttg
     4081 ccggagccgg cggagtgctg caccaggtag cgattgcccg ggccttcctc gcgggccgtc
     4141 tggagcagtt cggtgacgag ttcccactgg tggaagcgcg ggaagagcag agtggtgtgc
     4201 tttgtcacct cgcccgtgat cgggtcggtg gactgggact tctcgatgtg cagcagccgg
     4261 ccgaggatgt tcagccaggc gtcgcgctgc agcacgcgtt cccacaggta ggaggaggcg
     4321 gcgccgtccg ggttcaccgg attgcccgcg ccctgatcgc ggcccaggtt gaacggcagg
     4381 aacctcgtgg cggcgcctgc gaggcgggtg gtcatcgcga cctcgtcggt ggagaccgcg
     4441 aagtgcacca gggcgcggtt cgcgaagccg agcaggggct cccccttcgg gtcgcggtca
     4501 tggcggtact gggcgatcgc atcgtcgagc ttctgcatgt tgtcggtctt gagctcgagc
     4561 gtcgcgaccg ggatgccgtt ggcgaacagc acgaggtcga tggagttgct gttctttgcg
     4621 gagtagtaga cctgccgcac gacgcgcagc cgtaccgccg catagtcctg catcgctttc
     4681 gcgttgcgcg tggtggccgg cttgaaggcg cacatccgga aggtcgcggg ggtcttcttg
     4741 aacccgcgac gcagcacgtt gaggactccg ccgccggagt cgagggggag gtcgagcgtc
     4801 ttcacgagcc ggtcgaggag ctggtgcttc gccttgccct gctccgcaac ggatgctgtt
     4861 ggcttgacga ccttggccca ctcgccgggc tgggtctgct gaatccaggc gaagacatcc
     4921 tcggggaaga gcgcgcgggt cttgtcgtag ccgtgggtgt tctcgggctc gtagatccac
     4981 ccgttcgccg cgaggtgctc gcagatctcg cgttcgaacg cttcctcgtg ctggatgccc
     5041 atgcatgtaa gcatggcgca tatgagtcat gccaccttcc cttagggcgc actacaatgt
     5101 agttcatgtc atccacgccg ctgtccatcc gcttcgacga tgcggtgctc gatcgtgtac
     5161 gccgttccgc gagcgcgcgg gacgtgacgg cgtcgagcct gatccagcgc tatgtggagg
     5221 aagggctgcg cacgcaggac ttcccgggca tcgtgttccg ggatggaccg tcggggcggc
     5281 gagccgcact cgccgtcggc ccggacgtct gggagatcgc atccgcgctg cgggatgcgg
     5341 gcgcgagagg ggatgccgcg gtgagggcgg tggccgtgga cttcgctctg ccggagtcag
     5401 cggtgcgggt cgcgttggcg tactacggtg cgtattccgc ggagatcgat tcggaggtgg
     5461 cggagaatga tcgcgccgcc gaggaggcat tgcggtcgtg gcaggcgcaa cgccggctcg
     5521 tctcgtgacg tcgctgctgc ttctcgatga gcagtactcg gatgtcatcg cggtggcact
     5581 gcgtgggcgc ggccacgatg tggtgtcggt cgtcgccgac gcgggattgc gtggcgtgag
     5641 cgacgccgag ctgttccact gggcggcgag tgccggtcgg cggatcgtca ccgagaacat
     5701 caaggacttc cgcccgctgc tagtcgcggc cctggcttcg ggagcgccga tcgcgccgct
     5761 gctgctcgtg ccgccgcgga ggttcccgcg gagcgtggac gcgatcatcg cggctctggg
     5821 tgcatggctg gagaatacgg agccccacgt gcgcccggtg gaggactggc tggtctgaat
     5881 cctgggggcg cgttggatgg ggatcggctg acccgagaaa gttctgctga tcagcctcga
     5941 tgacgagctt tcaatgtcgg tggccccctg ttgaatagag gtatgaacag caccgcagca
     6001 gtcctcgatc gggtcgcctc cgacctcgat ctggtactgg gagcggatgc actttcgacc
     6061 ctgacggatt ccgagcggct ctcggtgctg aacgccgccg gtgaagtgct gaggcgcgtc
     6121 gaggctgttg tcgtcgagac ggtggcgacg tccgacccgg cggatgctcc aaacagcgcc
     6181 ggatgccgca gcatgaacga actgctgcag cgcactcttc gggtcgactc ccgcacagcc
     6241 gcgcagatgg cgaaggcggg aaggctcacg catcgcgaca cagacctcac atcaggtgag
     6301 tccctgcctg cgaagtggcc cgccctgcgg caggcgcttc tcgacggtga catcggggtc
     6361 accggtctgc tggccgcgac cggcccaatc gaggccgccg gagatcgcat cagcgcagca
     6421 gaccgatgga aggctgatgc gctcctcgcc gacgtggcgc gcggatacgc gatgagcgac
     6481 gacgacgggg tggggcagga tgcggctccg gcgccgacgc ctgaggatct gaaggcgctc
     6541 gcgcacgaga tcgcggtgcg gctcgatccc gacggcgccg agccgatcga ggagcgcgcg
     6601 atgcgcaagc gctacctcac ggtcggacgg atgcgcgacg gcgtgtaccc gatgcgcggc
     6661 aacctgctgc ccgagacgta cgcccagctg cagctgctgc tcgacgcgca gctcaacccc
     6721 aagaccgagg ggccaccggt tccggagggc gtcgcgttcc ggccctccgc gggtgagacg
     6781 gatgccgtcg aagacgagtt caacagtgac ccgcgcagcg tgctcgacac gcgcacgcgc
     6841 gggcagaagc agcacgacgc gctggccgcg gctctcggga tcgctgcgcg ccactccgag
     6901 atgccttcgc tgggtggtgc ggcaccgacg ctggtcgtgc acatcgactt cgatgacgac
     6961 tcctgggcgc gcatcccggg agtcgatggc ccggtcagcg gtgccgtcgc gcgccacacc
     7021 ggctgcaccg gtgcgctgca acgcgtgttc accgagaagg ggcggatcgt cggaatgacg
     7081 atcaccgatc gtatcttcga cgtgtaccag cgccgcgcga tcacccttcg cgacaaggaa
     7141 tgcctgatcc cgggctgtca cgttcccgcg tcctggtgcg agatccatca cgtcatcgag
     7201 cacgcgcgcg gcggaccgac cagcaccgac aacggcgtgc cgctgtgctg gtggcaccat
     7261 cggtcactgg atcattccgg gtgggagatc cggatgaacg acgggctgcc cgagatccgc
     7321 ggcccgtcct ggtgggaccc gctccagcgc tggcgcgtcc cgcgcctgtc gctcccgcag
     7381 ttcgcgttca cgtagcgcgg tggtcgctgc ggtggcgggc tgcgggatcg tcgcttcgct
     7441 cgcagagcgg ggacccctcc gcttcgctcc tccccgatgc tcgctcacgc gacgatccct
     7501 ccgccctcag gcgtctgcgg gggataccac cacgggcatt gagcgagcgc agggagtcga
     7561 agcggcggat gctacgccgg gatctccagc tgcccggtga cggcggccgt gatcagcgcg
     7621 gcgcggcgtt ctttggagag ctcgatgaag cgctgcgcct tggcgatgag ggtgtcgatc
     7681 ttcgtcgtag ccgccgtaat tgcatctgct atctccttct gttccgggag tggtggcaga
     7741 gggattacgg cttcaccgag ttcttccctg ttgaccttag gcattttcac gcgcatggag
     7801 gtcatcgttg cgaaggcgag gaacggattg gagagaaggt aatacgcgag atagtcgcgg
     7861 tcgaaactgc ttgcggcact cagcgcatac atgtcggcgc tgcagagacc gtcttcgctt
     7921 gcgacggcca ccttgttcaa cgcggggcga atcttggagt acaagatctg tcctgccttc
     7981 acgacatact tgccgctgtc cgcgccttgc tccatcgcag tctccctgcc gatgatgcgg
     8041 ccagtaccgg attctatgtg atttggtgcg aagaggatgg aatcccgcca tcgttcagtg
     8101 cggggatcaa ccagcccacc agaaattgaa atgacgcggc caaatcgggc agtgctccag
     8161 gatgcgttct tgactccgag gaacggatgt tgtgcggttt cgactgtggg atccaggccc
     8221 tctaatgaga gctgcgtgac ggttgcggag cgacgctcgc gtagggtggc gatgagctgc
     8281 tcctgcttcg cgatgagcgt gtcgatccgg gcggtctcgc ggtcgaggta gtcggcgatc
     8341 gctaactgct cgtcgaaggg cggcaaaggt atctcgatcc cggcgatgtg ctgcagattg
     8401 aggccgtcgc gcttgtcacc tcctgcaagg ttgcggatcg actggtagtt ggagacgagc
     8461 cagtagtgca gatagcggta ctcgagcgtc ccgcccggaa taatggcggc catcgactga
     8521 ttggtcgtcg ccgggatctc tagtcgggcg gctgtccctt tggtcttgcc ctgccctgcg
     8581 aggcctacga ccacagatct cggcggaacc cagcgagcgc tgcttccgct gaggcccgct
     8641 tcactgatga attctgaggg cttcgtgatc gaccaatcgt tgacggatcc cgaattgagc
     8701 catggaacgg ttccgtcacg ccagtaatcc gcgttgtttc gatctggtgt gccacctgcg
     8761 taaatgctcg tgatccagcg aaggtgggag gcggtccaat gctcaggcaa gtcgggtgcc
     8821 caagcaattc cgtgacccga gctcaccgct ccacttccct gagcagctcc atgatctcgc
     8881 cgacgagctg gttcagctcg gcgtcgatct cctccagcgg gcggggcggc acgtactcgt
     8941 agaagtgcct cgtgaacggg atctcgtagc cgaccttcgt cttggtcgtg tcgacccagg
     9001 catccggaac atgcgggagc acctcggcgg cgaagtacgc ctcgatgaca gcgtcccggg
     9061 catccgctcc cgcctcgttg ccaccccagg tgaacgggac gttctcggtg tcgcggagct
     9121 tggaatccgg cttcggattg cccttgcggt cgcgaacgat ctcgccgtcc tcgtcgcgca
     9181 gcgggcgctc gacggtgatg gtccagtagc cgaaggcatc cgtggggaag atcttggagt
     9241 gcttctgctc gacgtgctcc gcgtagaggc gggcgatctc ggcgcgatcc tgcttcgcga
     9301 cctcacggtt cttctggccg aggttcttgc gcagcttgct ccagtacccg gtgccgtcga
     9361 tcagctgcac ggtgccgata cggtcagccg gcttgttgtt gtcgagcagc cagacgtagg
     9421 tggcgatgcc ggtgttgtag aacatgttcg tcggcaacgc gacgatcgcg tccaccaggt
     9481 catgttccag cagccagcgt cggatctgac tcggccccga ctcggcggcc ccggtgaaga
     9541 gcggtgatcc gttcaacacg atcccggcgc gtccgccgcc ttcgtgcacc ggccgcatct
     9601 tcgtggcgat gtgctgcagg aacagcatct ggccgtcgct gatcggcggg agcccaccgg
     9661 ggaagcgact gcgctcgccg gtctcctgga tgtgcgcctt caccgccgcc tgcgacgcct
     9721 tccagtccac gccgtacggc gggttggaga ggcagtagtc gaaggtctgc ccggcgaaca
     9781 gatcgtcgga gagggtgttc ccgagccgga tgctggtggc gtcctgcccc ttcgcgatca
     9841 tgtcgctctt gcagatcgcg taggactgcg ggttgatctc ctggccgtag agtcgcagct
     9901 tggcatccgg attcatctcc agcagccgtt cctcggcgac ggagagcatc ccgccggtgc
     9961 cggccgtcgg atcgtagatg gagcgaaccg cgcccttctc cgtgaggccg gagtcgtcgc
    10021 cggcgaacag cagatcgacg atcaggcgga tggcgtcacg cggagtgaag tgctcgccgg
    10081 ccgtctcgtt ggatgcttcg gcgaacttgc ggatcagttc ttcgaacagg tcgcccatgg
    10141 cggcgttcgg cattgcgtca ggggagaggt cgacttcggc gaaccgctgc acgaccagca
    10201 gcaggcggtt gctctcggcc agggcctcga tctgatcctc gaacttgaac cgctcgaaca
    10261 cgtcgatggc cttcgagaag ccggagatgt agtcgatcag gttcgactgg aggccctgcg
    10321 ggtcgccgag cagtcgagtg aaggtccagg gcgaggagtt gtagaagccg agtccggtct
    10381 tctgccggat gcgcacatcg cgcaggtctt cattcgatgt gctcaggagc acctcgtcga
    10441 tggcctcgcg gtgcgcggac atcacgccgt cgaggcgccg caggatcgtc atcggcagga
    10501 tgacatcgcc gtactggtgg ggtttgaagg ggcctcggag ctgatcagcg atctgccaca
    10561 cgaacgatcc gagactgctc accgatagcg cccttccaag ctgacacgtc gcctcgcgag
    10621 ctgtcagggg aggcgctcga gcgggcagaa ccgcttaacc atctcgtata tgagatacgc
    10681 atgcaagccg tcgtatgtac gagtacccgc agcatgactt cggcttggat gcttgacgga
    10741 tgaatgcgtc gcgctcacag gcatccgaga agctcgggat gctggtgcgc agactccgtg
    10801 agaagagtgc gatgagcagg cagacgcttt cagagaggtc ggggctgaac tcctcgcatc
    10861 tggcgcgcat cgaatcggcg aagggaaccc gacgctgttc gcgctgatcc agctggcgac
    10921 cgccttggag cagaagccgg agatcttcgt cgagggcctg tccgcggctg atctgccggc
    10981 ggacatcaag aagccctatt cggaagccga cttccgccgg gagctgcggc ggcgcgaagg
    11041 aggcgcctga cgcgcgcgac gcgtcggagt agccgccgcg gttctctccg ggcgtgtcag
    11101 gaggtcgggc agccctccgc ggtcggatcc tcgtcgcagg tgtgtgcgac gagggcagtg
    11161 cgggcctcgt agatctggat gctctgcggc ggaccaggga tggtgagggt tccgctgatg
    11221 gggaagtagc ccgtgccgag gtcgatctcc gcggtgtaga cggtgtcgac ggatgcctgg
    11281 taggtgccgc gctccgcgta ggtgtggctg gtgtcggtgg gcgtgaactg gggcacgcgc
    11341 agcgcctccc aggtctttcc ggcggtcgtg gcggtgcggg tctgtccgtc gccgtagcgg
    11401 aacacgaagg atgccggtgt gaaccgggcc gtgacgggat agccgaagag cgtccctgtg
    11461 cgggtgtgcg cggccgcgtc ggtggtgaag ttggtgggca gccccgcgac gccgaggttg
    11521 tcgggttcgc cggtgagcgt gccgggcgtg ggggagaagg acgcgaggtc ggtgatcgtg
    11581 atcggcggga tgtcgacatc gtcgggtgcc gcaggggtga cgacgctgta tgaaccacgc
    11641 gggatgcaaa cagagtctgc tccgccgaat attgcggcgc acttcttgta cgcctcggtc
    11701 tccttgtagg actgagaatc gggggctctg cccttcgagc tcttctccgg tttcgcctga
    11761 ccggacgaat ggttgtgtga gccgtcctta gtgttcgtgc ccccgacctg cgcggtgtcg
    11821 tcggtcagtg ttgtgttcgg gcatcgaccc agcgcaagct tgatctcctt cgggcatcca
    11881 ggctcaactg ccatcgctgg tgggacaacc cagatggcca tcgcgacgag caacccggct
    11941 agaagtggtg ctgtcagttt gcgcattgcg catcatcctc atcgagttcg gccgtgatga
    12001 cgtagcctgt gtcgatccac ctcatctcaa ctttctgcgc aagcacagtc ggccggtcga
    12061 tcggagttat gtcttcgtcg gcggcattga tggtggtgat gtccgaagca tcaaggcaga
    12121 caatggcggc cagcctttcg ccatgcagct caactgagcc aatgatgggg acgaacttgg
    12181 acacttttgc tgtcccgatg gtcttcaacc cggcagcttc gagatcctgg cgtcctcgga
    12241 tgtcagctgc aagggcactg cctgcaagaa agtcttgcgg gtcgggattc tcggcgccgt
    12301 tgcgtcgggc atttcccgcg tcgttgtacg cccggtacgt ctcttccgcc gccttgaatg
    12361 cttcctcgtc cgttgcgaac agcgctgtct tcgtgggctt cggatcgggc tccggcgtgg
    12421 gggtgcatgc cgacagcatc ccgagggcga gcagcccggc ggacagtgcg ccgagcgcgc
    12481 ggacggtgga ggtggcgtga gtcacccggc cacggtaacc cgaaccggca tatcgccgca
    12541 ggagttatcc accggtgggt gctgggcgca ctcgaaattc gagggtcaga aggcggggtc
    12601 gccgtggatc ggagcatccc agccgaacgg tgccgggcgt cgggtggaaa acgccggtat
    12661 gtgcgcggat tgtccgcagg tttccatccg gcccgtcttg gctgagatgg aaactgcccg
    12721 gtccaggtgt cgggtacccg cactttccat ctcaagtccg cgccggcaga tggagaacgt
    12781 cggtgagcgg gcgtgctcac cgacgttctg catctccttc ggagacagcc tcaggcggtg
    12841 tacccgtccg ccacggtcag ggcctcgtcg atcgcggaca ggccgcgcac cagctcctcc
    12901 tcggtgatca ccagcggcgg ggcgatgtgc gtgcggttga agtgcacgaa gggccagacg
    12961 cccgccttct tcgcggcagc ggcgaaggcc gccatcgggg cagcatccgc acccgacgcg
    13021 ttgaacggca ccagcggctc gcgcgtctcg cgattccgca ccagctccac agcccagaac
    13081 aggccgctgc cgcgcacctc gccgacgctc gggtgcgtct ccgcccaccc gcgcagggtc
    13141 ggctcgacga tccgctcgcc caggtcgcgc acgcgctcca ggatgccgtc gcggcggaac
    13201 acctcgaacg tcgccacgcc caccgcgcag gccagcggat gcccggagta tgtgagcccg
    13261 cccgggaagg agacctcgtc gaagtgcgct gcgatgcggt cggagatgac cacgccgccc
    13321 agcggcacgt aaccggagtt gacacccttg gcgaaggtga tcaggtcggg ctgcccgccg
    13381 taggcctgga agccgaacca ttcgcccacg cggccgaagc cgaccatcac ctcgtcggcg
    13441 atgtagacga tgccgtatcg gtcgcacagc tcgcgcacgc cctgcaggta gcccggcggc
    13501 ggtgcgagca cgccgttggt gccgacgacc gactcgatga tgatcgccgc gatcgtggac
    13561 ggcccctcga gctgcacggt ctgctccagg tgcgcgagtg cgcgctcgcc ctcctgctcc
    13621 tccgactcgg agcggaacgc cgagcggtac gcgtacggcc cgaagaaccg caccgctccc
    13681 gcatcgctga cctcgttcgc ccagcgccgc gggtcgccgg tcagcgagat cgcggtggag
    13741 gtggagccgt ggtagctgcg gtacatcgac agcaccttgc ggcggccggt ggtctggcgc
    13801 gccatgcgca ccgcgtattc attggcatcc gctccgccgt tggtgaagaa caccttctcg
    13861 aagccgtcac cggccacctc ggagatgagc cgggccagct cgccgcgcac gtcgttcgcg
    13921 agggcgggct ggacggtcgc aaggcgcccc gcctgctgct ggatggcggc gatcaggtcc
    13981 ggatgctgat gcccgagatt caggttgacc agctgactcg agaagtccag gtaggcgttg
    14041 ccctggtagt cccagaacgt cgagccctcg cccgccgcga caggcatcgg gtcgatgagc
    14101 gcctgtgcgc tccaggagtg gaacacgtga gcgcggtcat cggcccgcac gagggcctct
    14161 tcctcggggg caggcagtgg atgcgtgagg ccgtggcggt cggtgaactg tgccatgatc
    14221 gtgcgccttt cagtggttgc tcgggaagcc gaggtcgagc tgggcggggg tgtggtcggg
    14281 ccagcgggtg gtgaccacct tggagcgggt gtagaagccg atcgactcgg ggccgtagat
    14341 gtgcgagtcg ccgaacagcg agtccttcca gccgccgaac gagaaggcgc ccaccggcac
    14401 ggggatcggc acgttgatgc cgaccatgcc gacctcgatg tcgtactcga actgacgggc
    14461 ggtgccgccg tcgcgggtga agaccgctgt gccgttgccg aaccggttgg cgttgatcag
    14521 ctcgacggcc tcctcgtagc tgttcacgcg gaccaccgag aggaccggtc cgaagatctc
    14581 gtcctggtac accttcatct cggtggtgac gtggtcgatc agcgagacgc cggtgaagaa
    14641 tccgtcgccc tcgaacgcgc gctgcgtgcc gtcgaccacg accgtgccgc cttcggcggc
    14701 agcgcccgcc acgtaggagg cgaccttgtc gcggtgctcg cgggtgatca gcgggcccat
    14761 ctcgctggcc gggtcggtgc cggggccgat gcgcagctcg ccgatgcggg agcggatgcc
    14821 ctccaccagg tcgtccgcga tgtcgccgac ggcgaccagc accgacacgg ccatgcagcg
    14881 ctcgccggcg gagccgtacg ccgccgagat cgcggccgtc accgctccgt cgatgtcggc
    14941 atccggcatg acgaccatgt ggttcttggc gccgccgagg gcctgcaccc gcttgccgtt
    15001 cgcggaggcg cgctcgtaga tggagcgggc gatgggcgtg gaaccgacga agctcaccgc
    15061 gccgatgtcc ttcgagtcga gcagggcgtc gaccgcgacc ttgtcgccgt tgacgacgtt
    15121 gagcacaccg tccggcaggc cggcctcctg gaacgccctg gccagccaga tcgccgccga
    15181 cggatccttc tcgctgggct tgagcaccac ggcgttgccg cacgcgatcg cggatgcgac
    15241 catccacagc ggcaccatga cggggaagtt gaacggggtg atgcaggcga ccacgcccac
    15301 cggctgcttg accgagtgca cgtccactcc tcgggacacc tgctcggcgc gctcgccctt
    15361 caggtggtgc agcagtcctg cggcgaactc gacgttctcg atgccgcgcg agacctcgcc
    15421 ggcggcatcc gagagcacct tcccgtgctc gctggtgacg atggcggcga gttcgtcggc
    15481 gcgctcggtg aggatctggc gcagccggaa gaacacgtcg gcgcgcttga cgagcgacga
    15541 tgagcgccag gcgcgctgcg cctccttggc gaccgcgatc gcgtgctcga cctccgccgt
    15601 gctggccgcg gcgacctggc ggtgcgtctc gcctgtggcg gggtcgtaca ccggaagagt
    15661 gcggtcgact gtgccggcgt aggtgccggc gatgaagtgg cggatggtgc tcacaggatg
    15721 ctcctcatcg agagttcgtg gtcgttgtgt tcgtcgagtg gccgcacaga atgaccggcg
    15781 gcttgcttcc catgctgaca gcggcaagac tgacgtcatg cccgcagatc gtcaggactt
    15841 cgccgacaat cggacagatc gtccggatgc tgccgcgctg ccgcgcgtgc gcgatgtgct
    15901 cgacgccgag gtgctgctgc gcggcgaccc gcaggtgctc gtcggcggcg acgcgctg
//
LOCUS       DATMSX010000124         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_162652,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000124 DATMSX010000000
VERSION     DATMSX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162652"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VN041_03885"
     CDS             <1..784
                     /locus_tag="VN041_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWU28220.1"
                     /translation="TALGDDVAAVWIETPSFTGAVETRLREISEAAHAAGAIVVVGTD
                     PVGYGVLTPPALHGADIVTGDIQSLGLHQWFGGAHAGFIAVHDDPRFVMEMPSRLFGL
                     ESTDVPGEYGFGDVAYDRTSFAHREDGKEWVGTAAALWGIAAGVYLSLMGPEGMREHG
                     ELLLARTRYAQQRLASIPGIRLGDDALHLREFTIRLDGISAGDAVAALRREGIEPGVV
                     VDEHTLLVCVTDVTSASDIDRLADALAAAVSPASDITKEQVA"
     gene            781..2391
                     /gene="gcvPB"
                     /locus_tag="VN041_03890"
     CDS             781..2391
                     /gene="gcvPB"
                     /locus_tag="VN041_03890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWU28221.1"
                     /translation="MSLPIAPKTSPRRFQQASWDEPIVFELSTPGERGIAITPVEPAV
                     REAVGDVVADLPEGMRRPQPPALPEIAQMRVLKHYLRLSQENLGSDLNVDIGQGTCTI
                     KYSPKINDQLIEVPQLRAIHPAQDPADTQGALEMIWRLEKLMAEISGMDAVSLASPGG
                     SAGIWTNIAMIRAYHESRGEGEQRDEVITTIFSHPSNAAAAKVAGYKVVTVYPDADGY
                     PDLGALKAALSERTAAIMVTNPEDTGIYNPAIKEWVDAAHEVGALASYDQANAAGILG
                     ITRARDAGFDVCQFNLHKTFSAPHGCGGPGAAANAVSEALAPFLPGPRVEQIDGRYVV
                     REPGELSIGHVAPAHGVILHVVRAYAWIMSLGAEGMLAASQIAVLNNNYLLKRVLEIP
                     GASAPYAVGRRRIEQVRYSWQELFEETGITSEEIGIRMADYGFHYWTSHHPYIVPQPF
                     TLEPTECYSKAELDEYVAALAQVAQEARENPELVRTAPHNQTVHHTHHDDLDDPERWA
                     ITWRAYQRKYFGVVRADAAREPAMSTVA"
BASE COUNT          383 a          913 c          825 g          341 t
ORIGIN      
        1 caccgcgctg ggcgacgacg tcgccgcggt gtggatcgag acgcccagct tcaccggcgc
       61 cgtcgagacg cgactgcgcg agatctccga agccgcccac gccgccggtg cgatcgtcgt
      121 ggtcggcacc gaccccgtcg gctacggcgt gctcaccccg cccgcgctgc acggcgctga
      181 catcgtcacc ggcgacatcc agtcgctggg cctgcaccag tggttcggcg gcgcgcacgc
      241 cgggttcatc gcggtgcacg acgacccgcg gttcgtcatg gagatgccgt cccggctgtt
      301 cgggctggag tccaccgacg tccccggcga gtacggcttc ggcgatgtcg cctacgaccg
      361 cacgtcgttc gcgcatcgcg aggacggcaa ggagtgggtc ggcaccgcgg cagcgctgtg
      421 ggggatcgcc gccggcgtgt acctgtccct gatgggaccg gagggcatgc gcgagcacgg
      481 tgagctgctg ctcgcccgca cccggtacgc ccagcagcgc ctggcgtcga tcccgggcat
      541 ccgcttgggt gacgacgccc tgcacctgcg ggagttcacc atccgcctcg acggcatctc
      601 ggccggcgac gcggtcgccg cgctgcggcg agagggcatc gagcccggcg tcgtcgtcga
      661 cgagcacacc ctcctcgtct gcgtgaccga cgtcacctcc gcctccgaca tcgaccggct
      721 cgccgacgca ctcgccgcag ccgtgtcccc ggcatccgac atcacgaagg agcaggtcgc
      781 atgagcctcc ccatcgcacc caagacgagc ccgcgtcgct tccagcaggc aagctgggac
      841 gagccgatcg tcttcgagct gtccaccccg ggtgagcgcg gcatcgcgat caccccggtg
      901 gagcccgccg tccgagaggc cgtgggcgat gtggtcgccg acctccccga gggcatgcgt
      961 cgtccgcagc cgccggcact gcctgagatc gcgcagatgc gcgtcctcaa gcactacctg
     1021 cgcctctcgc aggagaacct cggctccgac ctgaacgtcg acatcgggca gggcacctgc
     1081 acgatcaagt attcgccgaa gatcaacgac cagctcatcg aggtgccgca gctgcgcgcg
     1141 atccaccccg cgcaggaccc tgctgacacg cagggcgccc tggagatgat ctggcggctc
     1201 gagaagctga tggccgagat ctccggtatg gacgccgtct cgctggcctc gccgggcggc
     1261 tcggccggca tctggacgaa catcgccatg atccgcgcgt accacgagtc ccgcggcgag
     1321 ggcgagcagc gcgacgaggt catcaccacg atcttcagcc acccgtccaa cgcggcggcg
     1381 gcgaaggtcg ccgggtacaa ggtcgtcacg gtctacccgg atgccgacgg ctaccccgac
     1441 ctgggagcgc tgaaggcggc gctgtccgag cgcaccgcgg ccatcatggt caccaacccc
     1501 gaggacaccg gcatctacaa ccccgccatc aaggagtggg tcgacgccgc gcacgaggtc
     1561 ggtgcgctgg cctcctacga ccaggccaac gccgccggca tcctgggcat cacgcgcgcc
     1621 cgcgacgccg gattcgacgt gtgccagttc aacctgcaca agaccttctc cgctccgcac
     1681 ggctgcggcg gacccggtgc ggccgccaac gcggtcagcg aggcgctcgc cccgttcctg
     1741 cccggtccgc gggtcgagca gatcgatggc cgctacgtgg tccgcgagcc gggcgagctg
     1801 tcgatcgggc acgtggcccc cgcacacggc gtgatcctgc acgtggtgcg cgcctacgcc
     1861 tggatcatgt cgctgggcgc ggagggcatg ctggcggcct cgcagatcgc ggtgctgaac
     1921 aacaactacc tgctgaagcg ggtgctcgag atccccgggg catccgctcc gtacgccgtc
     1981 ggacgccgcc gcatcgagca ggtgcgctac tcgtggcagg agctcttcga ggagaccggc
     2041 atcacgtccg aggagatcgg catccgcatg gcggactacg gcttccacta ctggaccagc
     2101 caccacccgt acatcgtgcc gcagccgttc acgctggagc cgacggagtg ctactccaag
     2161 gccgagctcg acgagtacgt cgccgccctc gcgcaggtcg cgcaggaggc gcgcgagaac
     2221 cccgaactgg tgcgcaccgc gccgcacaac cagacggtcc accacaccca ccacgacgat
     2281 ctcgacgacc cggagcgctg ggccatcacc tggcgcgcat accagcgcaa gtacttcggc
     2341 gtggtgcgtg cggatgccgc gcgcgagccc gcgatgtcga cggtggcctg agcgtcgtga
     2401 tcggactggt cgtcaaccct gtcgccggag tcggcgggcc cgcgggcctg gccggctccg
     2461 ac
//
LOCUS       DATMSX010000125         1907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172428,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000125 DATMSX010000000
VERSION     DATMSX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172428"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1359)
                     /locus_tag="VN041_03895"
     CDS             complement(43..1359)
                     /locus_tag="VN041_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HWU28222.1"
                     /translation="MASKTELLQAQAFNRRRLQTAFTSGAPGGRELPPGKPLRGVVVS
                     IALGILTIIVSLLIGTFTGSLPKDWSKGAIIVVQGEGSRYVAIGETLYPVRNLASARL
                     LSGTTKITSVPASKLDDIKRDPMPVGIEDAPDYLPAPDRQYTGAWLSCVASDAPLEIS
                     TRLLGDRPGAANQAALVKDDDGGYWYVQGDRRYKVDKENVAVVASVFLGIDDVQAVPT
                     VSALWLNLVTPGTPLAVHLGGELKAETGTAGLLVGQAVQTQADGKVVGEYIVDGKGRL
                     VPATPFARQLLTAYVGVKPVSMTVAEATDLRDVNSDALPGDWPKREPVKPDNPSDVCL
                     QMQSTSEGPVVSVTNSPGELEPGTKVKPGAGVAVSARGTGEGSTYGFVSEAGVFFPVE
                     TATDLQLLGYKTTQAVTVPAAWTQLLEQGPTLSQAIAQRTAPGQAK"
     gene            complement(1363..>1907)
                     /locus_tag="VN041_03900"
     CDS             complement(1363..>1907)
                     /locus_tag="VN041_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HWU28223.1"
                     /translation="GIAGLGAPWMALASVPLRVVSARDDSEVYDAPAHISPEQVAKLY
                     VRAHRYQVSLRIALCLIVLVATVPVVASGFWGLLLCAVTYAGMFLGTRQVYSRFDIVI
                     VVAGVLLGVALVIVAAVISHPGWIGGLVIALAVAAVCVIVIVLLSSKVRLGLTRFADF
                     AEWATIALLLPLAIIAGGWF"
BASE COUNT          297 a          678 c          666 g          266 t
ORIGIN      
        1 cgccgacgac agcggccgca ctgcgcagag cgcgcccgcg catcacttcg cctgccccgg
       61 cgccgtcctc tgcgcgatcg cctggctcag ggtcgggccc tgctcgagca gctgcgtcca
      121 ggcggcgggc acggtcactg cctgcgtggt cttgtacccc agcagctgca ggtccgtggc
      181 ggtctcgacc gggaagaaca cgcccgcctc ggagacgaag ccgtaggtgg atccctcgcc
      241 cgttccgcgt gcgctgaccg ccacgccggc acccggcttg accttggtgc ccggctccag
      301 ctcgcccggg ctgttcgtca ccgagacgac cgggccctcc gaggtcgact gcatctgcag
      361 gcacacgtcg gacgggttgt cgggcttgac gggctcccgc ttcggccagt cgcccgggag
      421 cgcgtcggag ttcacgtcgc ggaggtccgt cgcctcggcg accgtcatcg acaccggctt
      481 gacgccgacg tacgcggtga ggagctggcg tgcgaacggc gtggcgggca cgagccgtcc
      541 cttgccgtcc acgatgtact cgccgacgac cttgccgtcg gcctgcgtct gcacggcctg
      601 gccgacgagc aggcccgccg tgcccgtctc ggccttcagc tcaccgccga ggtgcactgc
      661 cagcggcgtc cccggcgtga ccaggttgag ccagagcgcc gacaccgtcg gaaccgcctg
      721 cacgtcgtcg atgccgagga agacgctcgc gacgaccgcc acgttctcct tgtcgacctt
      781 gtaccgccga tcaccctgca cgtaccagta gccgccgtcg tcgtccttca cgagcgcggc
      841 ctgattcgcc gctcccggcc ggtctccgag caggcgggtc gagatctcca gcggcgcatc
      901 ggaggccacg cagctgagcc aggcaccggt gtactgccga tccggggcgg ggaggtagtc
      961 gggggcgtcc tcgatgccga ccggcatggg atcgcgcttg atgtcgtcga gcttgctcgc
     1021 cggcaccgag gtgatcttcg tcgtaccgct cagcaggcgg gcgctggcca ggttcctgac
     1081 cgggtacagc gtctcgccga tggcgacgta gcgcgatccc tcgccctgca cgacgatgat
     1141 cgcgcccttg ctccagtcct tcggcaggct gccggtgaac gtgccgatca gcagggagac
     1201 gatgatcgtc aggatgccga gggcgatgct gaccaccacg ccgcgcagcg gcttgcccgg
     1261 gggcagctca cgcccgcccg gcgctccgct cgtgaacgcg gtctggagcc gacggcggtt
     1321 gaaggcctgg gcctgaagca gctcggtctt ggatgccatg gatcagaacc agcctccggc
     1381 gatgatggcc agggggagca gcagggcgat cgtcgcccac tcggcgaagt cggcgaagcg
     1441 ggtcaggccc agtcgcacct tcgagctgag cagaacgatc acgatgacgc agacggccgc
     1501 gacggccagt gcgatgacca gaccgccgat ccagccggga tgggagatga cggccgcgac
     1561 gatcacgagg gcaaccccga gcagcacacc cgcgaccacg atcacgatgt cgaagcggga
     1621 gtacacctgc ctggtgccca ggaacatgcc ggcgtaggtc accgcacaca gcagcagacc
     1681 ccagaacccg gaggccacga cgggaacggt cgcgaccagc acgatcaggc agagcgcgat
     1741 gcgcagcgac acctggtagc ggtgcgcgcg gacgtagagc ttggcgacct gctcgggcga
     1801 gatgtgcgca ggggcgtcgt agacctcgga gtcgtcccgc gccgagacga cgcgcagcgg
     1861 cacggacgcc agcgccatcc acggggcgcc gaggcccgcg atgccgg
//
LOCUS       DATMSX010000126         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_68177,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000126 DATMSX010000000
VERSION     DATMSX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68177"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VN041_03905"
     CDS             complement(<1..802)
                     /locus_tag="VN041_03905"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWU28224.1"
                     /translation="MSEAFLVDGIRTPVGRYGGALSSVRPDDLAALVVGETVSRSGVP
                     HDAIDEVILGAANQAGEDNRNVARMAVLLAGLPDTLPGITVNRLCASGMSAITMASQA
                     IRSGDADVIVAGGVESMSRAPWVQAKPDRAWAKPGAAFDTSIGWRFANPKLVARDKAT
                     FSMPETAEEVARVDGISRADADAFALLSHQRALAAIDAGRLAAEIVPVETARGIVDTD
                     EGPRPETSLEVLAKLRPVVAGGEVVTAGNASSLNDGASAIVVASADAVQ"
     gene            1000..2337
                     /locus_tag="VN041_03910"
     CDS             1000..2337
                     /locus_tag="VN041_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28225.1"
                     /translation="MANTIDRRREERRVLAGTLVGTTIEWYDFFIYAQAAGLVFGELF
                     FAPLGATGGQIASWASLGISFLFRPLGAVIAGHLGDRLGRKRMLVLTLILMGASTSLI
                     GVLPTYADIGIMATVLLVLLRVLQGFSAGGEWGGAALMSVEHAPKERRGFFGAYPQIG
                     VPVGMILATFTMWVLNLAIGPEAFLAWGWRLPFLFSIVLIVVGYLIRRSVEESPVFLE
                     LQERRRESSAPLSMLFKHHTREVVLTALIFIANNAAGYLLIAFFTTYVSTVIGMPRPT
                     ALLMTTLASFGWLIFTMAGGWVSDRIGRVRTFQIGYGLLAVWSVPMWFLIDTGNVGLF
                     FVALFVLTIGLGLSYGPQSALYAEMFPADVRYSGVSIGYALGAILGGAFAPLIAELLL
                     KNTGASWSIGVYILGITIVSLVAVSFVKETRGAPLDTQSIEVVAARASGTVTR"
     gene            complement(2407..>3183)
                     /locus_tag="VN041_03915"
     CDS             complement(2407..>3183)
                     /locus_tag="VN041_03915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWU28226.1"
                     /translation="GVTISAPAAASASPTASAATAGGELDGLAVLSRERMSPLRKAVS
                     AKLSRSRAEIPEATVWVDVDATELWNLRAQMAPEGGKAPSLTALIARYALIALAEYPL
                     LASRLSEDATEIISFEGVNLGIAADTDRGLMVPVIPKAHGLSVEELDAALRELAATAR
                     AGGMPPERLRGSTFTLNNYGGFGVDGSTPIINHPDVAMLGIGRMIERPWVVDGSIVAR
                     RIAQISLVFDHRVCDGGYAAGFLRRVVELIEHPLRAYGRV"
BASE COUNT          437 a         1100 c         1102 g          544 t
ORIGIN      
        1 gctgcacggc atccgcgctg gcgacgacga tcgccgaggc gccgtcgttc agggagctgg
       61 cgttgcccgc ggtgacgacc tccccgcctg cgaccacggg gcgcagcttc gcgagcacct
      121 ccagtgaggt ctccgggcgc gggccctcat ccgtgtcgac gatgccgcgg gcggtctcga
      181 ccgggacgat ctcggcggcg aggcgaccgg cgtcgatcgc ggcgagggcg cgctggtggc
      241 tcagcagtgc gaaggcatcc gcatccgctc tgctgatccc gtcgacgcgg gcgacctcct
      301 cggcggtctc gggcatcgag aaggtggcct tgtcgcgggc gaccagtttg gggttcgcga
      361 accgccagcc gatcgaggtg tcgaacgccg cgccgggctt ggcccacgcg cggtcgggct
      421 tggcctgcac ccacggggcg cgggacatgg actccacgcc gccggccacg atcacgtcgg
      481 cgtcccccga gcggatggcc tgggaggcca tcgtgatcgc ggacatgccg gatgcgcaga
      541 ggcggttcac ggtgattccg gggagggtgt cgggcaggcc cgccagcagc accgccatgc
      601 gggcgacgtt gcggttgtcc tctccggcct ggttggccgc gcccaggatc acctcatcga
      661 tcgcatcatg cgggacgccg gatcgggaga cggtctcacc cacgacgagc gcggcgagat
      721 cgtccgggcg cacgctcgag agggcgcctc cgtagcgccc cacaggcgtg cggatcccat
      781 caaccaggaa ggcctcggac atcgtcatcc tttcgcgcgc gctcggcgcc aatagcagac
      841 cgatcgttca gtaatacgat ggcagatcat cgcgcggctg gcaacccgac ggcgatatgt
      901 ttccggtcga aaggcgcgcg gcccgcgccc cggatgcggt agtctcccga tttgctgacc
      961 catcggtcag tttccgctac ccgaacaacg gagttcccca tggcgaacac gatcgacaga
     1021 cggcgcgagg agcgccgggt cctggccggc acactcgtcg gcacgaccat cgagtggtac
     1081 gacttcttca tctacgccca agcggccggg ctcgtcttcg gcgagctgtt cttcgctccg
     1141 ctgggggcga ccggcggcca gatcgcgtcc tgggcatcac tgggcatctc gttcctgttc
     1201 cggccgctgg gggccgtgat cgcgggccac ctcggcgatc gtctcgggcg caagcggatg
     1261 ctggtgctca ccctcatcct gatgggcgct tccacctcgc tgatcggcgt gctgccgaca
     1321 tatgccgaca tcggcatcat ggcgaccgtg ctgctcgtgc tcctgcgcgt gctgcagggc
     1381 ttctcggccg gcggtgagtg gggcggggcc gcgctgatgt ccgtcgagca cgcgccgaag
     1441 gagcgccggg gcttcttcgg cgcctacccg cagatcggcg tgccggtcgg catgatcctc
     1501 gccaccttca ccatgtgggt gctgaacctc gccatcggcc ccgaggcgtt cctcgcctgg
     1561 ggctggcgcc tgccgttcct gttctcgatc gtgctgatcg tggtcggata cctcatccgt
     1621 cgctcggtcg aggagtcgcc ggtcttcctg gaactgcagg aacgccgccg ggagtcctcc
     1681 gcgccgctgt cgatgctctt caagcaccac acgcgtgagg tcgtcctcac tgcgctgatc
     1741 ttcatcgcga acaacgccgc cggctacctg ctcatcgcgt tcttcacgac ctatgtatcg
     1801 accgtcatcg gcatgcctcg tccgaccgcg ctgctgatga ccaccctcgc ctccttcggc
     1861 tggctgatct tcacgatggc cggcggatgg gtgtcggacc ggatcggccg cgtgcggacc
     1921 ttccagatcg ggtacggcct gctggccgtc tggtccgtcc cgatgtggtt cctcatcgac
     1981 accggcaacg tgggactgtt cttcgtcgcg ctgttcgttc tgaccatcgg actcgggctc
     2041 tcgtacgggc cgcagtcggc gctctacgcg gagatgttcc ccgcggacgt gcgctactcg
     2101 ggcgtctcga tcggctacgc gctgggcgcg atcctgggtg gcgcgttcgc tccgctgatc
     2161 gccgagctgc tgctcaagaa caccggtgcg tcctggtcga tcggcgtgta catcctcggc
     2221 atcacgatcg tgtcgctggt ggcggtgtcg ttcgtgaagg agacgcgggg tgccccgctc
     2281 gacacgcaga gcatcgaggt cgtcgccgcg cgggcgagcg gcactgtcac gcgctgaccg
     2341 ccggatgacg agtgagggct gatcgggatt cccgatcagc cctcactctg tccgccggaa
     2401 gcgcggtcag actcggccgt aggcgcggag cggatgctcg atgagttcca ccacgcggcg
     2461 caggaacccg gcggcgtaac cgccgtcgca cacccggtgg tcgaacacga gcgagatctg
     2521 cgcgatgcgg cgagccacga tcgacccgtc gaccacccaa gggcgttcga tcatgcggcc
     2581 gatgccgagc atcgccacat cggggtggtt gatgatcggg gtggagccgt ccacgccgaa
     2641 accgccgtag ttgttgagcg tgaacgtcga gccgcgcagc cgctcgggcg gcatcccgcc
     2701 ggcgcgtgcc gtggcggcca gctcgcgcag tgcggcatcc agttcctcga cgctcagccc
     2761 gtgggccttc gggatgaccg gcaccatcag gccgcgatcg gtgtcggccg cgatgccgag
     2821 gttcacgccc tcgaacgaga tgatctctgt ggcatcctcg ctcagacgcg acgccagcag
     2881 ggggtactcg gccagggcga tcagcgcgta gcgggcgatc agagcggtca gcgaaggcgc
     2941 cttgccgccc tcgggagcca tctgcgcgcg caggttccac agctcggtcg cgtcgacgtc
     3001 gacccagacg gtcgcctcgg ggatctcggc gcggctccgc gacagcttgg cgctgacggc
     3061 cttgcgcagc gggctcatcc gctcccggga cagcaccgcg agaccgtcca gctcccctcc
     3121 ggccgtcgcg gcggatgctg tgggcgatgc ggatgccgcg gcgggtgcgg agatcgtgac
     3181 ccc
//
LOCUS       DATMSX010000127         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_96713,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000127 DATMSX010000000
VERSION     DATMSX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96713"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN041_03920"
     CDS             <1..214
                     /locus_tag="VN041_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU28227.1"
                     /translation="LAPPAPPVGGRMPDSDPDAVLVAGPGIRHLIGGRVSPAEALAQD
                     LVHIARGNAKTLDSFAETFRIDPVAA"
     gene            295..918
                     /locus_tag="VN041_03925"
     CDS             295..918
                     /locus_tag="VN041_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU28228.1"
                     /translation="MTGRVIIDLFTTLDGVAQAPGGAEEDTRDGFPYGGWQAGYPSAA
                     VGASVTKGMETLDALLLGRRTYDIFAGYWPHHTTGPEGFIGQLFDRVPKYVASRDADI
                     QLDWQGSTRIGADLASEIAALREKHQDVHVIGSIDLVQTLLAEQLYDELQLWVYPVVL
                     GQGRKVFPDGAAPSNMRLLSADSGDGGTLSLRYAPVPGEVRTGTMGS"
     gene            complement(929..1336)
                     /locus_tag="VN041_03930"
     CDS             complement(929..1336)
                     /locus_tag="VN041_03930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWU28229.1"
                     /translation="MSDTTVIRTDEAPAPAHVFSQGLRKGNMLQVSGQGPMDPATNKY
                     VAEGDVHAQTIRTLENIRAILEAGDASVEDVIMFRVYLTSQDLFPAMNEAYGEFVQKY
                     VPSGMLPCRTTVFVGLPHPEMLVEIDALAVLSR"
     gene            complement(1407..>1661)
                     /locus_tag="VN041_03935"
     CDS             complement(1407..>1661)
                     /locus_tag="VN041_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWU28230.1"
                     /translation="DGALSLVDGEVFEQNVTAVVERDPVGAGDAFAAGYLHGVLVGSA
                     PQDRLDFAGRVAAHSVTLPGDWEGLPRLADLDVGGPDILR"
BASE COUNT          244 a          551 c          609 g          257 t
ORIGIN      
        1 gctcgctcct cccgcgccgc cggtcggcgg gcggatgccc gactcggatc cggatgccgt
       61 gctggtcgcc ggccccggca tccggcacct catcggcgga cgggtctcgc cggcggaggc
      121 actcgcccag gatctcgtgc atatcgcgcg gggcaatgcg aagacgctgg actccttcgc
      181 ggagaccttt cgcatagacc cggtcgcggc gtagcgtctg accgggcgcc acgcatgccg
      241 gagcccggtg agacgggcat cccacgaatc gaaccaccga atgaggagag catcatgacc
      301 ggacgtgtca tcatcgacct gttcaccacc ctcgacggcg tcgctcaggc gccgggaggc
      361 gcggaggagg acacgagaga cggcttcccc tatggcggct ggcaggccgg ctacccgagc
      421 gcggccgtcg gcgcgagcgt gaccaagggc atggagaccc tcgatgcgct cctgctcggt
      481 cgccgcacct acgacatctt cgccggctac tggccgcatc acaccaccgg accggagggg
      541 ttcatcggac agctgttcga ccgggtcccg aagtacgtcg cctcgcggga cgccgacatc
      601 cagctggact ggcagggctc gacgcggatc ggtgcggatc tggcgtcgga gatcgcagcg
      661 ctgcgcgaga agcatcagga cgtgcacgtg atcggcagca tcgacctggt gcagaccctg
      721 ctcgccgagc agctctacga cgagctgcag ctgtgggtct acccggtcgt gctcggccag
      781 ggacggaagg tgttccccga cggcgcggcg ccctcgaaca tgcggctgct cagcgcggat
      841 tccggcgacg gtggcactct ctcgctgcgc tacgcgccgg tgcccggtga agtgcgcacc
      901 ggcacgatgg ggtcgtgacc ggcgcgcgtc agcgcgagag cacggccagc gcgtcgatct
      961 cgacgagcat ctccgggtgg gggaggccca cgaacacagt ggtgcggcag ggcagcatgc
     1021 cactgggcac gtacttctgg acgaattcgc cgtaggcctc gttcatcgcg gggaacaggt
     1081 cctgcgaggt caggtacacg cggaacatga tcacgtcctc gaccgaggcg tcgccggcct
     1141 cgaggatcgc gcggatgttc tcgagcgtgc ggatggtctg ggcgtgcacg tcgccctcgg
     1201 cgacgtactt gttggtcgcg gggtccatcg gcccctgccc tgagacctgc agcatgttgc
     1261 ccttgcgcag gccctgggag aagacgtgcg cgggggccgg ggcctcgtcg gtgcggatga
     1321 cggtggtgtc ggacatgtct gctccttgcg gatccggttg cggttcgggg ttgcggttcg
     1381 gggttgcggt tctcgggcgg ctcgggtcag cgcaggatgt cgggtccgcc gacgtccagg
     1441 tctgcgagcc ggggaaggcc ctcccagtcg ccagggaggg tgacggagtg cgcggccacg
     1501 cggccggcga agtcgagccg gtcctgcggt gcggatccga ccagcacgcc gtgcaggtag
     1561 ccggccgcga aggcgtcgcc ggctcccacc gggtcgcgtt cgacgacggc agtgacgttc
     1621 tgctcgaaca cctcgccgtc caccagtgac agggcgccgt c
//
LOCUS       DATMSX010000128         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_11217,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000128 DATMSX010000000
VERSION     DATMSX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11217"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VN041_03940"
     CDS             complement(<1..733)
                     /locus_tag="VN041_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWU28231.1"
                     /translation="MREVVVSTPSGRLAGRQDDGVLRFHGVPYAEAPVGERRFAAPVP
                     VQPWEGILSASAPGPIAPQPLSPLADVSRGMGEDCLRLDIVTPAVPGWRPVVVWLHGG
                     GYFAGSSLEPDSDGTRLSAEHDLVVVSVTHRLGILGFLDLEPVGVPGSGIAGHLDLLA
                     ALRWIRSHITAFGGDPERVTVVGHSGGGGKTSMLLSTPSAAHLMHGAVIMAGPEFDLN
                     DPSRAAATRTAVAEALGLDGTAEQLA"
     gene            complement(726..>1562)
                     /locus_tag="VN041_03945"
     CDS             complement(726..>1562)
                     /locus_tag="VN041_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU28232.1"
                     /translation="LRVGDLLFRTLSWGPEDGIPLLALHGFPQHAASWARVAPLLAEQ
                     GIRTLAFDQRGYSPGARVPLDQYTLDAVVGDVIGIADALGCDPLHLAGFGQGALQSWA
                     TAAAHPDRIASLTALRFPHPAAFARGVRTDAEQRRAWDALESMSPPRPAAEQLLRDDA
                     AALRSFLAGSGMRPELVDETTERLRDPEVLAAAVAWHLIPVEEMAQVPPVTVPALYIF
                     SEGPALLPATAELSAEYAHGGYRMVAFEQGGHWLLDTEAERVAEQIGRHVLDVAGGGA
                     SA"
BASE COUNT          237 a          578 c          557 g          190 t
ORIGIN      
        1 ccgcgagctg ctccgccgtg ccgtcgaggc ccagcgcctc ggcgacggcg gtcctcgtcg
       61 cggcggcgcg cgacggatcg ttgagatcga actccggccc tgccatgatc accgcaccgt
      121 gcatcagatg cgctgcggac ggcgtgctga gcagcatcga tgtcttcccc ccgcccccgg
      181 agtgccccac cacggtcacc cgctccggat cgccgccgaa cgccgtgatg tgcgaccgga
      241 tccaccgcaa tgccgcgagg aggtcgaggt gtccggcgat gccggagccg ggcacgccga
      301 cgggttcgag gtcgaggaac ccgaggatgc ccagtcgatg cgtcaccgag acgaccacga
      361 ggtcgtgctc cgcgctcagc ctggtcccgt cgctgtccgg ttccagtgag gagcccgcga
      421 agtatccccc gccgtgcagc cacaccacga ccggacgcca accagggacg gccggcgtga
      481 cgatgtccag gcgaagacag tcctcgccca ttccccggct gacatcggcg agggggctga
      541 ggggctgggg agcgatcggg ccgggcgcgg acgcggaaag gatgccctcc cagggttgca
      601 ccggaaccgg ggcggcgaac cggcgctccc ccaccggggc ctctgcgtag gggacgccgt
      661 ggaacctgag cacgccgtcg tcctggcgcc cggcgagacg acccgagggc gtcgacacca
      721 ccacctcacg cactcgcgcc tccgccggcc acatccagga catgacggcc gatctgctcc
      781 gcgacgcgct cggcctcggt gtcgagcagc cagtgcccac cctgctcaaa cgccaccatc
      841 cggtacccgc cgtgcgcgta ctccgcgctc agctccgccg tggccgggag cagggcgggc
      901 ccctcgctga agatgtagag cgccggcacg gtgaccggcg ggacctgcgc catctcctcg
      961 accgggatga ggtgccaggc cacggccgcg gcgagcacct ccgggtcgcg caggcgctcc
     1021 gtcgtctcgt cgacgagctc cgggcgcatc ccggatcccg cgaggaacga tcgcaacgcg
     1081 gcagcgtcat cgcgcagcag ctgctcggcg gcggggcggg gcggactcat gctctccagc
     1141 gcatcccacg ctcgacgctg ttcggcatcc gtgcggacgc cgcgcgcgaa cgccgcgggg
     1201 tgcggaaagc gcagcgcggt cagtgatgcg atccgatcgg gatgcgccgc agcggtcgcc
     1261 cagctctgca atgcgccctg cccgaaaccg gccaggtgca gggggtcgca cccgagcgca
     1321 tcggcgatgc cgatgacgtc gccgaccacg gcatccagcg tgtactgatc cagcggcacg
     1381 cgtgcgcccg gcgagtatcc gcgctggtcg aaggcgaggg tccggatccc ctgctccgcg
     1441 agcagcggcg cgacccgcgc ccagctggcg gcgtgctgag ggaagccatg cagggcgagg
     1501 agggggatcc cgtcctcggg gccccaggac agcgtgcgga acagcaggtc tccgacgcgg
     1561 ag
//
LOCUS       DATMSX010000129         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39816,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000129 DATMSX010000000
VERSION     DATMSX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39816"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..879
                     /locus_tag="VN041_03950"
     CDS             1..879
                     /locus_tag="VN041_03950"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28233.1"
                     /translation="MPPRTAARRGSAYLSTHPRTRLALLLSAPLFWLGAVYVVALALL
                     LVTAFWTTDTYTGEIRTEFTLDNVITVLTDGFYQGITLRTVGVALAVTAIDVLLAVPI
                     AYFMAMVASPAMQRALVVLVLTPLWASYLVKAFAWRSVLSNDGILEWLLRPLGGHTPG
                     YGIPATIITLAYLWLPYVILPVYAGLERVPNSLLEASGDLGGRSWTTLRLVVLPLAWP
                     AIIAGTIFSFSLSLGDYITVGIVGGGSQMLGTLVYQDIGAANNLPLASAIALIPIIII
                     FGYLFLVRRTGALDNL"
     gene            888..1688
                     /locus_tag="VN041_03955"
     CDS             888..1688
                     /locus_tag="VN041_03955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28234.1"
                     /translation="MRISLGTRITLGVIAAVVLVIMYVPLLVVLLNSFSTSASLSWPP
                     PGFTLEWWGRAFVSEGAMAAVGTSALVAVISAALSLVLGTLLSFALQRFSFFGRSTVN
                     LLVILPIALPGIITGIALNNLFRTIMGVPLSIWTVIIAHTTFCIVTVFNNAIARMRQM
                     GTNLEEASADLGAGIWTTFRLVTFPQLRSALLAGGLLAFALSFDEIVVTTFTAGSGVK
                     TLPMFIIDNVSRPNQAPIVSVIAVVLVLVSVLPIAMAQKLSGSEQQRR"
     gene            1738..3438
                     /locus_tag="VN041_03960"
     CDS             1738..3438
                     /locus_tag="VN041_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWU28235.1"
                     /translation="MTTFVPPRPWTASYAEGVPEDLAPVSGSLIDIVEASVRDYPDAP
                     ALQFFGAETTYRQMQDAIDRAANGLRELGVKAGDPVAIVLPNSPQHIIAFYAVLRLGA
                     VVVEHNPLYTPRELRKQFEDHGAKHAIVWSKVVQTVQEFPSDLAVDSLISVDVTRAMP
                     FMTRMLLKLPVAKARESREALTSKVRGAISWDAIVASAPLPTTHPRPQTDDLAIIQYT
                     SGTTGTPKGASLTHRNLLANAAQARAWVPSITRGNGCVVYAVLPMFHAYGLTLCLTFA
                     MSMGARLVLFPKFDPDLVLEVTKKHPATFLPLVPPIAERLLRAANEKGVSLKGTEVAI
                     SGAMALAHELVVPFEKASGGFLVEGYGLSECSPVLMANPVAENRVAGTVGLPLPGTEC
                     RVVDPENPTQDVPAGSAGELIVRGPQVFSGYYGKPEETEAVFIDGWFRTGDIVTIDEG
                     GFVRIVDRIKELIITGGFNVAPTEVEGALRQHPDVVDAAVVGLPSEHSGEEVVAAIVV
                     DGEKDVDVEAIREFARGILTPYKVPRRIFVVDELPKSLIGKVLRRQVKEKLVSLTSGS
                     "
     gene            3529..>4903
                     /locus_tag="VN041_03965"
     CDS             3529..>4903
                     /locus_tag="VN041_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU28236.1"
                     /translation="MTEETAKPGFEELGITGPVLKAVRDLGYETPSPIQAATIPLLLQ
                     GRDVLGTAQTGTGKTAAFALPVLENLDLSQKTPQVLVLAPTRELALQVCEAFEAYSAR
                     MKDVRVLPVYGGQAYGVQLSALRRGVHIVVGTPGRIMDHQAKGTLDLSELKYLVLDEA
                     DEMLKMGFAEDVEQILAQTPEDKQVALFSATMPATIRRLAQQYLNMPEEVAIKGKTTT
                     NQNITQRYLVVSYAQKVDALTRILEVENFDGMIVFVRTKNETETLAEKLRARGYSAAA
                     INGDVPQVQRERSVNQLKDGKLDILVATDVAARGLDVERISHVVNFDIPTDTESYVHR
                     IGRTGRAGRKGDAISFITPRERYLLKHIEKATRQQPEQMQLPTTEDVNTTRLNRFDDA
                     ITAALEEAGRIEAFRDIIAHYVRHHDVPEADVAAALAVVAQGDTPLLLDPERDELSRA
                     VERDNRPP"
BASE COUNT          756 a         1788 c         1542 g          817 t
ORIGIN      
        1 atcccgccgc gcaccgctgc gcgccgcgga tccgcctacc tgtccacgca cccgcggacc
       61 cgcctcgcgc tgctgctgtc ggcacccctg ttctggctcg gcgccgtcta cgtggtcgcc
      121 ctcgcgctgc tgctggtcac cgcgttctgg acgaccgaca catacaccgg cgagatccgc
      181 accgagttca ccctcgacaa cgtgatcacg gtcctcaccg acggcttcta ccagggcatc
      241 acgctccgca ccgtcggagt cgctctcgcg gtgacggcga tcgacgtgct cctggccgtg
      301 cccatcgcct acttcatggc gatggtcgcg tcgcccgcca tgcagcgcgc gctggtcgtg
      361 cttgtgctca ccccgctgtg ggcgtcgtac ctggtcaagg ccttcgcctg gcgctcggtg
      421 ctctcgaacg acggcatcct ggagtggctg ctccgcccgc tcggcgggca cacccccggc
      481 tacggcatcc ccgcgaccat catcacgctc gcctacctct ggctgccgta tgtgatcctc
      541 cccgtgtacg ccgggctcga acgcgtgccg aactcgctgc tggaggcgtc cggcgacctg
      601 ggcggccgca gttggaccac gctgcgcctg gtggtgctcc cgctggcctg gccggcgatc
      661 atcgcgggca cgatcttcag cttctcgctc tccctgggcg actacatcac cgtcggcatc
      721 gtcggcggcg gcagccagat gctcggcacg ctcgtctacc aggacatcgg ggcggcgaac
      781 aaccttcccc tggcatcggc catcgcgctg atcccgatca tcatcatctt cggctacctg
      841 ttcctcgtgc gtcgcaccgg cgccctggac aacctgtagg aggcgacgtg cgcatctcgc
      901 tcggaacccg catcaccctc ggtgtcatcg ccgctgtcgt gctcgtgatc atgtacgtgc
      961 cgctgctggt cgtgctgctc aactcgttct cgacctcggc ctcgctctcc tggcctccgc
     1021 ccggcttcac gctcgagtgg tggggcaggg cgttcgtcag cgagggtgcg atggcggccg
     1081 tcggcaccag cgcgctggtc gccgtcatct cggcggcgct ctccctcgtg ctcggcacgc
     1141 tgctctcctt cgccctgcag cggttcagct tcttcggccg cagcacggtc aacctgctgg
     1201 tgatccttcc gatcgccctg cccgggatca tcacgggcat cgccctgaac aacctgttcc
     1261 gcacgatcat gggcgtcccc ctgtcgatct ggacagtgat catcgcgcac accacgttct
     1321 gcatcgtgac cgtgttcaac aacgcgatcg cccggatgcg gcagatgggc accaacctgg
     1381 aggaggcgtc ggccgacctc ggcgcgggca tctggaccac cttccggctc gtcacgttcc
     1441 cgcagctgcg gtcggcactg ctcgccggcg gcctgctcgc cttcgcgctc tccttcgatg
     1501 agatcgtggt gacgacgttc acagccggct ccggggtcaa gacgctgccg atgttcatca
     1561 tcgacaacgt ctcccgcccg aaccaggcgc cgatcgtctc ggtgatcgcc gtggtgctcg
     1621 tgctcgtctc ggtcctcccg atagcgatgg cgcagaagct gtctggcagc gagcagcagc
     1681 gccgctgagc cggcccgcgt ggcgggcgtc gtcacctgcc cggcataagc tgggcgggtg
     1741 acgacgttcg ttcctccccg cccctggacc gccagttacg ccgaaggcgt tcccgaagat
     1801 ctcgcgccgg tctccggctc gctgatcgac atcgtcgagg cctcggtgcg ggactacccg
     1861 gatgccccgg cgctgcagtt cttcggtgcg gagacgacct accgtcagat gcaggatgcc
     1921 atcgaccgcg cggcgaacgg gctgcgcgaa ctgggtgtga aggcgggcga tcccgtcgcg
     1981 atcgtcctgc cgaactcgcc acagcacatc atcgcgttct acgcggtgct gcgcctcgga
     2041 gccgtggtcg tcgagcacaa cccgctgtac accccgcgcg agctgcgcaa gcaattcgag
     2101 gaccacggcg cgaagcacgc gatcgtgtgg agcaaggtcg tccagaccgt gcaggagttc
     2161 ccttcggatc tcgccgtcga cagcctgatc tcggtcgacg tcacccgggc gatgcccttc
     2221 atgacgagga tgctgctgaa gctgccggtc gcgaaggcac gggagtcccg tgaggcgctc
     2281 acatccaagg tgcgcggcgc gatctcgtgg gacgcgatcg tggcatccgc tcccctgccg
     2341 acgacgcacc ctcgcccgca gaccgacgac ctcgcgatca ttcaatacac atccggcacc
     2401 accggcacac ccaagggcgc ctcgctcacg caccgcaacc tgctcgccaa cgcggctcag
     2461 gcgcgcgcct gggtgccgtc catcacgcga gggaacggct gcgtcgtcta tgcggtgctt
     2521 ccgatgttcc acgcctacgg gctcacgctc tgcctgacct tcgcgatgtc catgggtgca
     2581 cggctggtgc tgttcccgaa gttcgatcct gatcttgtgc tcgaagtcac caagaagcac
     2641 ccggccacgt tcctccccct cgtgccaccg atcgccgagc gcctgctgcg cgccgcgaac
     2701 gagaagggcg tctcgctgaa gggcaccgag gtcgcgatct ccggcgcgat ggcgctggcg
     2761 cacgaactgg tcgtgccgtt cgagaaggcc tcgggcgggt tcctcgtgga gggctacggc
     2821 ctcagcgagt gctcccccgt cctgatggcc aaccccgtcg cggagaaccg cgtggccggc
     2881 acagtggggc tgccgctccc cggcaccgaa tgccgcgtcg tcgacccgga gaaccccacg
     2941 caggacgtcc cggcgggttc cgccggcgag ctcatcgtcc gcggcccgca ggtgttctcc
     3001 ggctactacg gcaagcccga ggagaccgag gcggtcttca tcgacggctg gttccgcacc
     3061 ggcgacatcg tcacgatcga cgaaggcgga ttcgtccgga tcgtcgaccg catcaaggag
     3121 ctcatcatca ccggcggctt caacgtcgca ccgaccgagg tcgaaggcgc tctgcgccag
     3181 catccggacg tcgtggacgc cgccgtcgtg ggtctgccca gcgaacactc cggcgaggag
     3241 gtcgtcgccg cgatcgtcgt cgacggcgag aaggacgtgg acgtcgaggc gatccgtgag
     3301 ttcgcccgcg ggatcctcac gccctacaag gttccgcgcc gcatcttcgt ggtcgacgag
     3361 ctgcccaagt cgctgatcgg caaggtgctg cggcgccagg tgaaggagaa gctggtctcg
     3421 ctgacctccg gttcctgaat cctcttcctg cggtcgtaca ggcgcaggca ctatcgttag
     3481 ctggtgactg ctgacgataa tgccgccgtg cccaccgact cctccgccct gaccgaggag
     3541 accgcgaagc cgggcttcga agaactcggc atcaccggcc ccgtgctgaa ggccgtccgc
     3601 gacctcggct acgagacgcc ctcgcccatc caggcggcga cgatcccgct gctgctgcag
     3661 ggtcgcgacg tgctcggcac agcgcagacc ggcaccggca agaccgccgc gttcgcgctg
     3721 cccgtgctgg agaacctcga tctgtctcag aagacgccac aggtgctggt gctcgcgccg
     3781 acgcgcgagc tcgcgctgca ggtgtgcgag gcgttcgagg cgtactccgc ccgtatgaag
     3841 gatgtccgcg tgctcccggt gtacggcgga caggcctacg gcgtgcagct ctccgccctt
     3901 cgccgtggtg tgcacatcgt cgtcggcacc cccggccgca tcatggatca ccaggccaag
     3961 ggcacgctcg atctctccga gctgaagtac ctcgtgctgg atgaggccga cgagatgctg
     4021 aagatgggct tcgccgaaga cgtcgagcag atcctcgccc agacacccga ggacaagcag
     4081 gtcgcgctct tctccgcgac catgcccgcc acgatccgcc ggctcgccca gcagtacctg
     4141 aacatgcccg aagaggtcgc gatcaagggc aagaccacga ccaaccagaa catcacgcag
     4201 cgctatctcg tggtgtccta cgcgcagaag gtggacgccc tgacccgcat cctcgaggtg
     4261 gagaacttcg acgggatgat agtcttcgtg cgcacgaaga acgagaccga gacgctcgcc
     4321 gagaagctgc gcgcccgtgg ctactccgct gcggcgatca acggcgacgt gccgcaggtg
     4381 cagcgcgagc gcagcgtgaa ccagctcaag gacggcaagc tcgacatcct cgtcgccacc
     4441 gatgtcgccg ctcgtggtct ggacgtggag cgcatcagcc acgtcgtcaa cttcgacatc
     4501 ccgaccgaca ccgagtcgta cgtgcaccgc atcggccgca ccgggcgggc gggtcgcaag
     4561 ggcgacgcga tcagtttcat caccccgcgc gagcgctacc tgctcaagca catcgagaag
     4621 gcgacgcgcc agcagccgga gcagatgcag ctgccgacca ccgaggacgt caacaccacc
     4681 cgcctgaacc gcttcgacga cgccatcacc gccgccctcg aggaggccgg ccggatcgag
     4741 gcgttccgcg acatcatcgc gcactatgtc cgccaccacg acgtccccga ggcggacgtg
     4801 gcggccgcgc tggccgtcgt cgcgcagggc gacacgcccc tgcttctgga cccggagcgc
     4861 gatgagctgt cccgagccgt cgagcgggac aaccgtcccc cgc
//
LOCUS       DATMSX010000130         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39834,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000130 DATMSX010000000
VERSION     DATMSX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39834"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VN041_03970"
     CDS             complement(<1..823)
                     /locus_tag="VN041_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWU28237.1"
                     /translation="MATRPIGTSVVRAINARAAFRLLLDRGPLSRPEIASALAVSKPT
                     ASHLLAQLRESGLIKSIGNRDGGIGRAAELFSVDGGVAHAAAVDVTPERIAAVVVDLT
                     GTRVGENVLPTSDQATPDPGAQVLAVLQGACDGTDVEPAGLQRVVVGIQGAIDPTCDR
                     LRFAAHLPGWQGAALTSTLQDAIGTTVDIENDVNLVTIAEHARGAAVDHDSFAELWVS
                     DGIGLGLMFGGRMLRGRTGGAGEISDLPLPGTPVPPDENEHGASGMQALAGAPALG"
     gene            998..3145
                     /locus_tag="VN041_03975"
     CDS             998..3145
                     /locus_tag="VN041_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HWU28238.1"
                     /translation="MRQRRPLIALLAAAVIIPLSGLAVGAAQADDLSTLRDPVKREIA
                     QEIITSAENSVLDWYNRYGYIEDIGDGRGYTGGIIGFTSGTSDMLELVERYTAKYPSN
                     PLAKYLPALRSVDGSDSHAGLGSAFVAAWEAEGDVPAFQRAQRDLTREWYFEPSVDLA
                     LEDGLNALGQFAYYDAAVVHGFDGMEDIRAAAKGDASTPAEGGNESTYLNKFLDERVV
                     EMKKEEAHEDVSRIENAQRVWLKAGNLDLDTPLSWSVYGDPFSIPTDPTPRWPLNEIG
                     DGTGTTPTPTPTPTSGVPGTVTLISKNRPTATSSTEGAGFEGAKAVDADATSRWASEE
                     GVDPQWIRVDLGTGATVSKVVLKWEAAYATKYRVEISPDGTSWSTLATESAGNGGTDE
                     FTNLTGTGRYLRVYGTARGTSYGYSLYDLSVFGTAGTDGGDPGGNPGSGAFTVAAAGD
                     IAGQCSTPDEDTCAHFLTADLAQSMDPEFYITLGDNQYDDGHLSDFQNYYSKSWGVFK
                     DKTHPVPGNHEKYDNDYDEDTDRGDEQAYRQYFGAQATPQGKSWYSYDQGNWHFIALN
                     SNLFYDDEQMDWLDADLAANTKPCTVAYFHDPLFTSGEHGNQLSSKPVWTKLEAAGTE
                     LVLNGHDHHYERFAPQTSAGATSDTGIVEIIAGLGGVNLRDIEDTPQKNSLFRYNSDY
                     GVLKLNLTDDSFTTQFVTIDGQVQDTSPTYSCH"
     gene            3150..4445
                     /locus_tag="VN041_03980"
     CDS             3150..4445
                     /locus_tag="VN041_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28239.1"
                     /translation="MYLAGSKARQDEGGSAVHLTVGRRAAGVGSTAVALGTVSLLTDL
                     SSEMVTAVLPLYLVVGLGMSPLQFGFLDGLYNGVTAFVRLLGGHVADRWSRHKLVAGI
                     GYGLSAVCKPFLLLAGANTTALSATLAVDRIGKGIRTAPRDALISLSVDDAHQGRAFG
                     VHRAMDTAGALAGPLAAFLLLWLVADAFDAVFMVSFCVATLGVLVLIAFVPGRSSMPI
                     GSVRPTLRQTVGILAEPRYRRLLIAATLLGMVTIGDGFLYLGLQDRMKLDTVFFPLLP
                     LGSAAVYLVLAVPFGRLADRIGRRLPFLLGNLALLGAYSTMLLPIDGPLLLVLTLGML
                     GLFYAATDGVLMALAGPGIPRDRRAGGLALLQTGQAVGRLLAAVLFGAAWTLWGLGAA
                     FSAASVALLAVVIVAKVLIRPDHGADEGVVPSSDAGSRA"
     gene            4445..5446
                     /locus_tag="VN041_03985"
     CDS             4445..5446
                     /locus_tag="VN041_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28240.1"
                     /translation="MVAGRSAVRSRMLMVAGAALVLAAIGVGYTAYAMNRPSAGSAPV
                     AGDATPAEHPGLYVRDAGGSIVVQSSDAKAGKGGEAPDRIGTGLNCARFYAEGGTAVC
                     LTTRLGVKPMTSAIILDDHLEQQQRFEFGGVPSRARVSGEGRFVSWTVFVTGDEYATT
                     DFSTRTGFYDLEQDFLVKSVEDLGLYIDGRLRSPEDVNYWGLTFADDQNTFYVTVSTG
                     GETYLAQGDLASWTAHTLRKNVECPSLSPDGTRIAFKKRVDPGVESPWRLYVLDLATM
                     KETAVAEGRSVDDQAAWLDDDTLAYAVDDGVWSVPSDGTGAPSLLAPGASSPAMVRP"
     gene            5495..5644
                     /locus_tag="VN041_03990"
     CDS             5495..5644
                     /locus_tag="VN041_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28241.1"
                     /translation="MTARVGGRSLAISWIVGLLCAATVAGLLWLALGAGPGLLFILEQ
                     MVGGG"
BASE COUNT          940 a         1996 c         2050 g          923 t
ORIGIN      
        1 gccccagtgc cggggcgccg gcgagggcct gcatgccgct ggcgccgtgc tcgttctcgt
       61 cgggcggcac aggagtgccg ggcaggggca ggtcggagat ctctcccgcg ccgccggtgc
      121 gtccgcggag catcctgccg ccgaacatca atcccaggcc gatgccatcc gagacccaca
      181 gctcggcgaa actgtcgtgg tccaccgccg caccgcgggc gtgctcggcg atggtcacga
      241 gattgacgtc gttctcgatg tcgacggtcg tgccgatggc gtcctgcagc gtggatgtga
      301 gtgcggcacc ctgccagccg ggcagatggg cggcgaaccg caaccggtcg caggtgggat
      361 cgatcgcccc ctggatcccg accacgacgc gctgcagccc ggccggttcc acgtccgtgc
      421 catcgcacgc accctgcagc acggcgagca cctgggcgcc tggatcgggc gtcgcctgat
      481 cggatgtcgg gagcacgttc tcgccgaccc tcgtgcccgt gagatccacg acgacggccg
      541 cgatccgctc cggcgtcacg tccacggccg ccgcgtgggc gaccccgccg tcgacgctga
      601 acagctctgc ggcccggccg atgccgccgt cgcggttccc gatgctcttg atgaggccgc
      661 tctcgcgcaa ctgcgcgagg aggtgagatg cggtgggctt cgacacagcc agtgctgagg
      721 cgatctccgg cctgctcagc gggccccggt ccagcaggag ccggaatgcc gcacgagcgt
      781 tgatcgcgcg gacgacgctg gttccgatcg gtcgagtggc cacggtgtcc ttctgaggat
      841 gagatcgatt gcacccggag tcgtgggagc ggttacacgg atgctatccg tcggcagacg
      901 gaacccctgg tctggaaaac ggttgccatc ggtatccgtg tcgggtataa ctcatcgtaa
      961 aggtgttttc caaactacga gcgataccgg aggaatcatg cgtcaacgga gaccactgat
     1021 cgcgctgctt gcggccgcgg tcatcatccc cctcagcgga ctggcggtgg gcgctgctca
     1081 ggcggatgac ctgagcacgc tgcgcgatcc cgtgaagagg gagatcgcgc aggagatcat
     1141 cacgtcggcg gagaactcag tgctggactg gtacaaccgg tacgggtaca tcgaggacat
     1201 cggtgacggt cgcgggtaca ccggggggat catcgggttc acgtcgggca cgagcgacat
     1261 gctcgaactg gtcgagcgat acaccgccaa gtatccgagc aatcccctcg ccaagtacct
     1321 gcccgcgctg cgttccgtgg acggcagcga ttcgcatgcc ggtctgggca gcgcgttcgt
     1381 ggccgcgtgg gaggcggagg gtgatgttcc cgcattccag cgggcgcagc gggacctgac
     1441 gcgggagtgg tacttcgagc cttccgtcga cctggcgctg gaggacgggt tgaacgctct
     1501 gggtcagttc gcgtattacg acgcggcggt ggtgcatggc ttcgacggga tggaggacat
     1561 ccgtgctgcg gcgaaggggg acgcgtccac gcctgcggag ggtgggaacg agtcgacgta
     1621 tctgaacaag ttcctggatg agcgcgtggt ggagatgaag aaggaggagg cgcatgagga
     1681 cgtctcccgg atcgagaacg cgcagcgggt gtggctcaag gccgggaacc tggatctgga
     1741 cacgccgttg agctggtcag tgtacggcga tccgttctcg atccccacgg atccgacgcc
     1801 gcgctggccg ctcaacgaga tcggcgacgg caccgggacg acgcccacgc cgaccccgac
     1861 tccgacgtcc ggagtgccgg ggacggtgac gctgatctcg aagaaccggc cgaccgcgac
     1921 gtcctcgacg gagggtgccg gcttcgaggg cgcgaaggct gtcgacgcgg acgcgacatc
     1981 ccgatgggcg agcgaggagg gcgtggatcc gcagtggatc cgcgtggacc tgggaacggg
     2041 tgcgacggtg tcgaaggtgg tcctgaagtg ggaagccgcc tacgcgacca agtaccgcgt
     2101 cgagatctcc ccggacggga catcgtggtc caccctcgcc actgaatccg caggcaacgg
     2161 aggcaccgac gagttcacga acctgacggg caccggccgc tacctgcgtg tctacggcac
     2221 tgcccgtggc accagttacg gatactcgct gtacgacctc tccgtgttcg gcaccgcggg
     2281 aaccgacggc ggcgacccgg gcggcaaccc cggctccggc gcgttcacgg tcgcagccgc
     2341 cggtgacatc gccggccagt gctccacgcc cgatgaggac acgtgcgcgc acttcctcac
     2401 cgccgacctg gcgcagtcca tggaccccga gttctacatc acgctcggcg acaaccagta
     2461 cgacgacggt cacctgtccg acttccagaa ctactactcc aagtcctggg gcgtcttcaa
     2521 ggacaagacc cacccggttc cggggaacca cgagaagtac gacaacgact acgacgagga
     2581 caccgaccgc ggagacgagc aggcctatcg ccagtacttc ggcgcccagg ccacgcccca
     2641 gggcaagagc tggtacagct acgaccaggg caactggcac ttcatagcgc tgaactcgaa
     2701 cctcttctac gacgacgagc agatggactg gctcgacgcc gatctcgccg ccaacaccaa
     2761 gccgtgcacg gtggcctact tccacgaccc gctgttcacg tcgggggagc acggcaacca
     2821 gttgagcagc aagccggtgt ggaccaagct cgaggcggcg ggcaccgagc tcgtgctgaa
     2881 cgggcacgac catcactacg agcgcttcgc accgcagacg tcggcgggtg ccacgtccga
     2941 cacgggcatc gtcgagatca tcgcgggcct cggtggcgtg aacctccgcg acatcgagga
     3001 cacgccccag aagaacagcc tcttccggta caactccgac tacggcgttc tgaagctgaa
     3061 cctgacggac gactcgttca cgacccagtt cgtcacgatc gacggccagg tgcaggacac
     3121 cagcccgacg tacagctgcc actgacgaca tgtatctcgc cggcagcaaa gcgcggcagg
     3181 acgaaggagg ctcggcggtc cacctcacgg tgggccgccg agccgccgga gtcggaagca
     3241 ccgcggtcgc cctcggaacg gtgagcctgc tcaccgatct ctcctccgag atggtgacgg
     3301 cggtcctgcc gctgtacctc gtcgtcggac tgggcatgag cccactccag ttcggcttcc
     3361 tcgacggcct ctacaacggg gtcaccgcgt tcgtgcggct cctgggcggg cacgtcgccg
     3421 acagatggtc gcgacacaag ctcgtcgcgg gcatcggcta cggcctgtcc gccgtgtgca
     3481 agcccttcct gctcctggcg ggcgccaaca cgaccgcgct gtccgccacg ctcgccgtgg
     3541 accggatagg caagggcatc cgcaccgccc cgcgtgacgc gctgatctcg ctgtcggtcg
     3601 acgacgccca ccagggccgt gcattcggcg tgcaccgggc catggacacc gccggcgcgc
     3661 tggcaggtcc cctcgcggcc ttcctgctgc tctggctggt cgccgacgcg ttcgacgcgg
     3721 tgttcatggt cagcttctgc gtcgcgacgc tgggcgtgct ggtgctcatc gctttcgtcc
     3781 cgggccgctc gtcgatgccg atcggcagcg tgcgacccac actgcggcag acggtcggca
     3841 tcctcgccga accgcgctac cgtcggctgc tgatcgcggc caccctgctc ggcatggtca
     3901 cgataggcga cgggttcctt tatctcggtc tgcaggaccg catgaagctg gacacggtgt
     3961 tcttcccgct gcttccgctc ggcagcgccg ccgtctacct ggtgctcgcg gtgcctttcg
     4021 gacggctggc cgaccgcatc ggacggcgtc tgccattcct gctcgggaac ctggcgctgc
     4081 tgggcgccta ctcgaccatg ctgctgccga tcgacggtcc cctgctcctg gtgctgacac
     4141 tcgggatgct cgggctgttc tacgccgcga cggacggggt gctgatggcg ctggcggggc
     4201 cgggcatccc gcgggaccgc agggccggag ggctcgccct gctgcagacc gggcaggccg
     4261 tcggacgtct gctcgcggct gtgctgttcg gtgcggcgtg gacactgtgg ggcctcggcg
     4321 cggcgttctc ggccgcatcc gtcgccctgc tggccgtcgt catcgtcgcg aaggtgctca
     4381 tccgccccga ccacggtgcg gacgagggcg tcgtcccgtc atcggatgcc gggagcaggg
     4441 cgtgatggtc gcgggacgtt ccgcagtgcg gagcaggatg ctgatggtgg cgggggcggc
     4501 actcgtgctc gccgccatcg gcgtcggcta caccgcctat gccatgaaca ggccgtccgc
     4561 aggcagcgcg cctgtcgccg gagacgcgac gcccgcggag catccgggtc tgtacgtgcg
     4621 ggatgccggg ggctcgatcg tggtgcagtc atccgatgcg aaggccggga agggcggtga
     4681 ggcgccggac cgcatcggaa cggggctgaa ctgcgcccgc ttctacgcgg agggcggcac
     4741 cgcggtgtgc ctgaccacgc gtctcggcgt caagcccatg accagcgcga tcatcctgga
     4801 cgaccacctc gagcagcagc agcgcttcga gttcggcggt gtgcccagca gggcgcgcgt
     4861 gtcgggggag gggcgcttcg tgtcgtggac ggtgttcgtg accggggacg agtacgccac
     4921 gaccgacttc tcgaccagga ccggcttcta cgacctcgag caggacttcc tggtgaagtc
     4981 ggtggaggac ctcggcctgt acatcgacgg ccggctccgg tcaccggagg acgtcaacta
     5041 ctggggcctc accttcgccg acgaccagaa caccttctac gtgacggtgt ccaccggcgg
     5101 ggagacctat ctcgcccagg gcgacctggc gtcgtggacc gcgcacacgc tgcggaagaa
     5161 cgtcgagtgc ccgtcgctct cgcccgacgg cactcgcatc gccttcaaga agagggtcga
     5221 ccccggcgtc gagtcgccct ggcgactgta cgtgctggac ctggcgacca tgaaggagac
     5281 ggctgtggcc gagggccgca gcgtcgacga tcaggcggcc tggctcgatg acgacacact
     5341 ggcatacgcc gtcgacgacg gggtctggtc cgtgccctcc gacggcacgg gcgcgccgag
     5401 ccttctcgcc ccgggggcgt cgtcgccggc gatggtgcga ccctgatcgg cgttgcgttc
     5461 gcccgtcgac gatcgccggg cgtaccgtga ccccatgacc gcacgcgtgg ggggaaggag
     5521 cctggcgatc agctggatcg tcgggctcct ctgcgcggcg acggtggcgg gtctgctgtg
     5581 gctcgccctg ggagcgggac cggggctgct gttcatcctc gagcagatgg tcggcggcgg
     5641 ctgacgtccc gccggaggcg aagcaccgac acgtcggcac gcctccgagg cgcgcgtttg
     5701 cgcgcgcgtt accctcgctg tgcaccttat ccaggagagg cgaggccatg agcgaccccg
     5761 aggttcccca gaacgacaag ccgaacgaca tcgacgacgt cgtcggaagc gccaacgagg
     5821 gtctcgacgc tgccgccgcg gcccgcgccg aaacccccgc tgactccgcc gagccgcact
     5881 ccgacgccga catggacgcg ttcgccgcc
//
LOCUS       DATMSX010000131        10126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_1959,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000131 DATMSX010000000
VERSION     DATMSX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VN041_03995"
     CDS             complement(<1..1459)
                     /locus_tag="VN041_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HWU28242.1"
                     /translation="MTSFAAPSLTWRDGRLFRDGEPHRILSGAVHYFRIHPDQWEDRL
                     RRLAAMGANTVDTYIAWNFHERTEGEFDFTGWRDVARFIRIAGEVGLDVYVRPSPYIC
                     AEWSNGGIPFWLSGRMRALRSSDAGFLAAVDAWFDALIPQLEPLQAVHGGPIRLIQVE
                     NEYGSYGSDQAYLAHQRDALVSRGMVEMLTTADGTTADMVRNGSVPGVMGTFTFGTGV
                     ALAQELRRDDHHLMCSELWGGWFDHWGEQHHVRSAESMIGTVQELLDAGGSVSIYMAH
                     GGTNFGLWAGANHDGVIQPTITSYDSDAPIGEDGSLNDKFHALRAAFAPFHGGELPEL
                     PDAPEHQPAASAALMPIASLLDVVRAQPVVATAPQPLTYEELGAEDGLVAYESDVVFE
                     ADTVLTLRELRDRATVFLDDERLGTIEHDGEQSLALPSAGGAGRLTIVVESLGRINYG
                     PLTGERKGILGGVLIGRRFQHGWEHRMLPRDAVPAA"
     gene            1555..2580
                     /locus_tag="VN041_04000"
     CDS             1555..2580
                     /locus_tag="VN041_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU28243.1"
                     /translation="MSPRRPSPDRAPNQLDVARTAGVSAQTVSRVLSGQQNVRPETAR
                     RVHAAVETLGYRIHAAAASLASGRTRILGMILVSTDRYSSAALGAGVEQAAAANGYTV
                     TMAAVPDHASTDAFVQAFDRLERQGAEGIVLGLPVELNSPAMRMRTDRTPSTRTERAS
                     LDMDAPLIVDQHAIARLAVEHLLDLGHSTVWHVPADDYWLESRQRRETWEQVLIERGI
                     TPPPVIPGDWTPESGYRAGRTIAAIPDATAVFVSSDEMAFGLIRALHEAGRRVPEDVS
                     VVSVDDIALAAFASPALTTVRQPFEGMGHAAASRLIAHIEGRDLEDVPDLHPELIIRS
                     STAAPRA"
     gene            complement(2648..6511)
                     /locus_tag="VN041_04005"
     CDS             complement(2648..6511)
                     /locus_tag="VN041_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-alpha-N-acetylgalactosaminidase family
                     protein"
                     /protein_id="HWU28244.1"
                     /translation="MKRTQRRWAGVGLTCAVVAAGLSAFSTAPAWAVGDGQVVLRTGV
                     LEVRASSAFPQVVQYIDRASGAVMRGNDSALTSIQINGTPQPVSVSSAQIDGSTVEYV
                     LTPTALDGVRLTARLSVKKNVVTLKFTDIQDHGKKVATLRIPGQNLVTVSSADEGSAV
                     AAANLSVDRAVSGDTFIPVNAQTPLNASPVSSNAVIANTARLAAAFTTNAMYDESSGP
                     ANKDSGNFWRQAVKDGDDVEVGISSGQWLYRAKGAPEPEELPWVKVAITPDANADGTV
                     DWQDGAIALRDLRVGPYKGEQTPDNVVTHIPFNFASQATHPFLRTLDDVKRIALNTDG
                     LGQVAMLKGYTSEGHDSANTDYGDNFNERAGGLDELNDALAAGKKWNASFGVHINATE
                     IYPEANSFSDDIMNPKSPGWNWLDQSYYIDQRFDTNSGRLDKRIGELADATNDNLDFV
                     YVDVYYQYGWLAKKIQESLVDHGFRVGSEWADKLPANNTWSHWANDESYGGSTNKGIN
                     SQILRFVNNTEADVWNPDPRLGTSHIVEFEGWTNQNDFNGFLKNVWTANVPAKFLQHH
                     EITRWTDEAIELKDGVRVTGTDAASRSITVGGAEVLHGGNYLLPWASKDGGKVDKLYH
                     YNPAGGSTTWTLTSQFAGNATMQLFSLGDGGRTKVADVPVVDGKVTIDAKAGQPYVLV
                     AGKKADAGSSLPKKTRFGESTPVVDPGFNETTLDAWSPTGEVTIERDGLGRRAAKIGA
                     GEASLSQRLGALPAGEYTASAWVGIDKNKTRDVKLGVQVPGGKDVGIAIDSSNAQNYV
                     AADEWHGTTMQRLKVPFTVSADGTRPTLTFSAGAGDAAVRIDDVRIITAAAPKLGGDV
                     IAREDFEDVDAGWGPFYKGAAGGSTDPRTHIAKRNDYTQKGWNGRQIDNILNGEYSLM
                     AHDENSGIVYRTSEYSVPFQAGHRYRVSFDHQSTSAGEYAWVTGYDAANGPRVTQRTS
                     IGAVHETTRFEQVIDASACGDTFVGLERIGGGSADLILDDFSVEDLGASDAIPACAQV
                     SGELGADVVQQGEATPFTTTFISDEGAPISDLAVTLGLPEGWTAEAVTAATAATLPAK
                     GTLTTKWNLYAPADADGSYDITVKGAYRTTVEPIGSRTATGTVTVKTLPAPPTKTVYA
                     SDHDWVTSSNGWGPVERDLSNGEQGQGDGTPLTLNGVVFAKGLGAHAPSNVKYFLGGN
                     CTVFTAKVGVDDVQTSRGSVVFSVKVDGRTVITSPLMKPTSETFDLTADVTGGQFVEL
                     VVGDGGDGNGNDHADWADAEFTCAP"
     gene            6805..10053
                     /locus_tag="VN041_04010"
     CDS             6805..10053
                     /locus_tag="VN041_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HWU28245.1"
                     /translation="MPHSSGHHSPIDDAQEASVLTTPISRRQALQVGGFAALAALTPT
                     FLAARPASAAVGAPHSAASAARSLPRPQLSRYALWYGTPAKEWESQALPIGNARLGAK
                     LFGDPTADVVQFNEQSMWGGVNDYDNALAGQGDGAYDTSVTGFGSYRDFGTVTVAFGG
                     TTTVTSPGGPYRTSGSEGVDRSYDGDSRTKWCIDGPPAQVLWQADLADATEVASYSLT
                     SANDVPARDPQEWTFQGSSDGSSWTTLDTRTGAPFESRFQTKTYTVANPGTYRHYRFV
                     FAPKAGVSHFQLSEIGLAGVALAGGDGWYVSSPSGHADSLLGSVDGDAGTVWQVGDAG
                     SGAIWQVESAAKRAFTGYTLTSAADDAAADPRDWTVAGSDDGLTWTTLHQTTGETFAE
                     RGQTRAFSFANTKAYAVYRIVFTGPSAFRVAGIAFTAGAFSTAGARTVVDYRRALDIA
                     DGTYSTHFATTGGTVLREAFASRDADVIVLRYSTDRAGSLNADIGMVSAQEVRTTADA
                     AKRRLSFAGTMANELSHAAVLQIADTDGEVAAAGDGLRVTGATSITLLLDARTDYLMD
                     AAKGWRGAAPLPGIEKALAAASARSFDELRSAHVTEVSALMSRAKVDWGSTADAVLAL
                     PTQRRLTRYAAGESDPTLEQTMYAYGRYLLLSSSKPGGLPANLQGLWNNSNQPAWASD
                     YHTNINIQMNYWGAETADLPEAHEALAAFIEQVAVPSRVATRNAFGDVRGWTARTSQS
                     IFGGNSWEWNTVASAWYAQHLYEHWAFTQDKRYLRDHAYPLIKEICEFWEDRLKTLPD
                     GTLVSPNGWSPEHGPREDGVMYDQQIIWDLFQNYLDCAEALDVDPDYRATVADMQSRL
                     APNRIGSWGQLQEWQTDRDDPKDIHRHTSHLFAVYPGRQITVDDTELAAAALVSLKAR
                     CGEKEGVPFTAGTVSGDSRRSWTWPWRAGLFARLGDAERAGIMVRGLLTYNTLPNLWA
                     NHPPFQLDGNFGITGAIAEMLLQSHEGAIRLLPAVPADWAAKGSFTGLRARGGYRVSC
                     EWQDGRVVSYDVVADRAPNMSNVVVIVNGQRRKVKPANPKAGLPMRDLGPAN"
BASE COUNT         1482 a         3527 c         3495 g         1622 t
ORIGIN      
        1 ccgccgcggg aaccgcgtcg cgcggcagca tccggtgctc ccagccgtgc tggaaccggc
       61 ggccgatcag cacgccgccg aggatgccct tgcgctcgcc cgtgagcggg ccgtagttga
      121 tgcgcccgag gctctcgacg acgatcgtga gccgcccggc gccaccggcc gagggcagcg
      181 cgaggctctg ctcgccgtcg tgctcgatcg tgccgaggcg ctcgtcgtcg aggaacaccg
      241 tggcgcgatc gcgcagctcg cgcagcgtga gcaccgtgtc ggcctcgaac acgacatccg
      301 attcgtaggc gaccaggccg tcctccgcac ccagctcctc gtacgtgagc ggctggggcg
      361 cggtggcgac gaccggctgc gcgcgcacga cgtcgagcag cgaggcgatc ggcatgagcg
      421 cggcggatgc agcgggctga tgctccggag catccggaag ctccggaagc tcgcccccat
      481 ggaacggagc gaacgccgcg cgcagcgcgt ggaacttgtc gttcagcgag ccgtcctcgc
      541 cgatcggggc atccgagtcg tacgacgtga tggtgggctg gatcacgccg tcgtggttgg
      601 ctccggccca cagcccgaag ttcgtgccgc cgtgcgccat gtagatgctc accgacccgc
      661 cggcatcgag cagctcctgc accgtgccga tcatgctctc ggccgatcgc acgtggtgct
      721 gctcgcccca gtggtcgaac cagccgcccc acagctcgct gcacatcagg tgatgatcgt
      781 cgcggcgcag ctcctgcgcg agggcgaccc cggtgccgaa cgtgaaggtg cccatcacgc
      841 ccgggacgct tccgttgcgc accatgtcgg cggtggtgcc gtcggctgtg gtgagcatct
      901 cgaccatccc gcgtgagacg agcgcgtcac gctggtgcgc gaggtaggcc tggtcgctgc
      961 cgtaggagcc gtactcgttc tcgacctgga tcaggcggat cggcccgccg tgcacggcct
     1021 gcagcggttc cagttgaggg atgagcgcgt cgaaccaggc atccacggcg gcgaggaagc
     1081 cggcatccga cgagcgcagc gcgcgcatcc tgcccgacag ccagaacggg atgccgccgt
     1141 tggaccactc cgcgcagatg tacgggctgg ggcgcacgta gacgtcgagg ccgacctcgc
     1201 cggcgatgcg gatgaagcgc gcgacgtcgc gccagccggt gaagtcgaac tcgccctcgg
     1261 tgcgctcatg gaagttccac gcgatgtacg tgtcgacggt gttcgcgccc atcgccgcga
     1321 gacggcgcag ccggtcctcc cactggtcgg ggtggatacg gaagtagtgc accgcgccgg
     1381 agaggatgcg gtgcggttcg ccgtcgcgga agaggcggcc gtcgcgccag gtgagcgagg
     1441 gggcggcgaa gctggtcata cgtctcctga gatgcggatg tttgcgttca cactatctca
     1501 tccagacccg ggaacaaccg cgggatcatg cggttgtgcc gctaccatcg ttccatgtca
     1561 ccacgtcgcc cgagccccga tcgggccccc aaccagctcg acgtcgcacg taccgccggg
     1621 gtcagcgcgc agaccgtctc gcgcgtgctc tccgggcagc agaacgtgcg tcccgagacg
     1681 gcccggcgcg tgcacgcggc agtggagacc ctcggctacc gcatccatgc cgccgcggca
     1741 tcgctggcct cgggccgcac ccgcatcctc ggcatgatcc tcgtctcgac ggaccggtac
     1801 tcctccgccg cgctcggcgc cggcgtggag caggcggccg cggcgaacgg ctacacggtc
     1861 acgatggccg ccgtccccga ccacgcctca accgacgcgt tcgtgcaggc cttcgaccgg
     1921 ctggagaggc agggcgccga gggcatcgtg ctcggcctgc cggtcgagct gaactccccg
     1981 gcgatgcgca tgcgcaccga ccgcaccccg tcgacgcgca ccgagcgcgc ctccctcgac
     2041 atggacgcgc ctctcatcgt cgaccagcat gcgatcgcac gactggccgt cgagcatctg
     2101 ctcgacctcg ggcactccac cgtgtggcac gtgcccgccg acgactactg gctcgagtcc
     2161 cgccagcgcc gcgagacgtg ggagcaggtg ctcatcgagc gcggcatcac cccgcctccg
     2221 gtgatccccg gcgactggac gcccgagtcc ggctatcgcg ccgggcgcac gatcgccgcg
     2281 atcccggatg ccaccgccgt gttcgtctcc agcgacgaga tggcgttcgg cctgatccgc
     2341 gcgctgcacg aggccggccg gcgcgtcccg gaggacgtct cggtggtcag cgtcgacgac
     2401 atcgcactgg cggccttcgc ctcgccggcg ctgacgacgg tgcggcagcc cttcgaaggc
     2461 atgggccacg ccgccgcgtc gcgcctcatc gcgcacatcg agggccgcga cctcgaggac
     2521 gttcccgacc tgcatccgga gctcatcatc cgctcgtcca ctgccgcacc acgcgcgtga
     2581 cgtgaaggag cggatgccgc ttgcgcagca tccgctcccg tgattcaccc gccctgtcgg
     2641 gccaccgtca gggtgcgcag gtgaactcgg cgtccgccca gtccgcgtgg tcgttgccgt
     2701 tgccgtcgcc gccgtcaccg acgacgagct cgacgaactg gccaccggtc acgtcggccg
     2761 tgaggtcgaa cgtctcgctg gtcggcttca tcagcggcga ggtgatgacg gtcctgccgt
     2821 cgaccttcac cgagaacacg accgatccgc gcgacgtctg cacgtcgtcg acgccgacct
     2881 tcgcggtgaa gacggtgcag ttgccgccga ggaagtactt cacgttgctc ggcgcgtgcg
     2941 caccgaggcc cttggcgaag accaccccgt tgagcgtcag cggcgtgccg tcgccctggc
     3001 cctgctcgcc gttggacaga tcgcgctcga ccggacccca gccgttgctg gaggtcaccc
     3061 agtcgtggtc gctggcgtag acggtcttcg tcggcggggc cggcagcgtc ttgaccgtca
     3121 ccgtgcccgt cgccgtccgc gacccgatcg gctcgaccgt ggtgcggtac gcgcccttga
     3181 ccgtgatgtc gtacgagccg tcggcgtccg ccggcgcgta caggttccac ttcgtggtca
     3241 gcgtgccctt ggcgggcagc gtcgccgccg tcgccgcggt cacggcctcg gccgtccacc
     3301 cctcaggaag gccgagcgtg acggcgagat ccgagatcgg ggcgccctcg tcggagatga
     3361 aggtggtcgt gaacggcgtg gcctcgccct gctggacgac gtcggcgccg agctctccgc
     3421 tcacctgcgc gcaggcgggg atcgcgtctg acgctccgag gtcctcgacc gagaagtcgt
     3481 ccaggatcag gtccgcgctg ccgccgccga tgcgctcgag gcccacgaag gtgtcgccgc
     3541 aggcgctggc gtcgatgacc tgctcgaaac gcgtggtctc gtgcaccgca ccgatcgacg
     3601 tgcgctgcgt gacccggggg ccgttcgccg cgtcgtaccc ggtgacccag gcgtactcgc
     3661 ccgcgctcgt cgactggtgg tcgaacgaca cccggtagcg gtgaccggcc tggaacggca
     3721 cggagtactc ggaggtgcgg tacacgatgc cggagttctc gtcgtgcgcc atgagcgagt
     3781 actcgccgtt caggatgttg tcgatctggc gaccgttcca tcccttctgg gtgtagtcgt
     3841 tgcgcttggc gatgtgcgtg cgcgggtcgg tgctgccacc ggccgcgccc ttgtagaacg
     3901 gcccccatcc ggcgtcgacg tcctcgaagt cctcgcgggc gatcacatcg ccgccgagct
     3961 tcggggcggc cgctgtgatg atccgcacgt cgtcgatgcg cacggcggcg tcgcccgcgc
     4021 cggcgctgaa cgtcaacgtg gggcgggttc cgtcagcact cacggtgaag ggcaccttca
     4081 gacgctgcat ggtggtgccg tgccactcgt cggccgccac gtagttctgc gcgttggagg
     4141 agtcgatcgc gatcccgacg tccttgccgc ctggcacctg cactccgagc ttcacgtcgc
     4201 gggtcttgtt cttgtcgatg ccgacccacg cgctggcggt gtactcgccg gcgggcaggg
     4261 cgcccagacg ctggctgagg gatgcctcgc cggcaccgat cttcgccgct cgccggccca
     4321 ggccgtcgcg ctcgatcgtg acctcgccgg tcgggctcca ggcgtccagc gtcgtctcgt
     4381 tgaatccggg gtcgaccacc ggggtgctct caccgaagcg ggtcttcttg ggcaggctgc
     4441 tgccggcatc cgccttcttg cccgccacga gcacgtaggg ctgcccggcc ttcgcgtcga
     4501 tcgtgacctt gccgtccacg accggaacat cggcgacctt ggtgcgaccg ccgtcgccga
     4561 gcgagaacaa ctgcatggtg gcgttgccgg cgaactggct ggtcagcgtc cacgtggtgc
     4621 tgccgcccgc ggggttgtag tggtacagct tgtcgacctt gccgccgtcc ttcgaggccc
     4681 acggcagcag gtagttcccg ccgtgcagca cttcggcgcc cccgacggtg atcgaccggc
     4741 tcgcggcatc cgtgccggtg acccgcacgc cgtccttgag ctcgatcgcc tcatcggtcc
     4801 agcgcgtgat ctcgtggtgc tgcaggaact tcgccggaac gttcgcggtc cacacgttct
     4861 tcaggaaccc gttgaagtcg ttctggttgg tccagccctc gaactcgacg atgtggctgg
     4921 taccgagtcg cggatcgggg ttccagacgt cggcctcggt gttgttcacg aagcgcagga
     4981 tctgcgagtt gatgcccttg ttcgtcgagc caccgtagct ctcgtcgttc gcccagtgcg
     5041 accaggtgtt gttggcgggc agcttgtcgg cccactccga gcccacgcgg aagccgtggt
     5101 cgaccagcga ctcctggatc ttcttcgcca gccatccgta ctggtagtag acgtcgacgt
     5161 agacgaagtc gaggttgtcg ttcgtggcat ccgccagctc gccgatgcgc ttgtcgagcc
     5221 gacccgagtt cgtgtcgaag cgctgatcga tgtaatagga ctggtcgagc cagttccagc
     5281 ccggcgactt cgggttcatg atgtcgtcgc tgaacgagtt cgcctcgggg tagatctcgg
     5341 tggcgttgat gtgcacgccg aacgaggcgt tccacttctt gcctgccgcg agcgcgtcgt
     5401 tgagctcgtc gagcccgccg gcgcgctcgt tgaagttgtc gccgtagtcc gtgttggcgg
     5461 agtcgtggcc ctcgctggtg tagcccttca gcatcgcgac ctggccgagc ccgtcggtgt
     5521 tcagcgcgat gcgcttgacg tcgtcgagcg tgcgcaggaa cgggtgcgtg gcctgcgagg
     5581 cgaagttgaa cgggatgtgc gtgacgacgt tgtcgggggt ctgctcgccc ttgtacgggc
     5641 ccacgcgcag gtcgcgcagc gcgatcgcgc cgtcctgcca gtcgacggtg ccgtcggcgt
     5701 tcgcgtcggg ggtgatcgcg accttgaccc agggcagctc ctcgggttcg ggtgccccct
     5761 tggcgcgata cagccactgg ccgctcgaga tgccgacctc gacgtcgtcg ccgtccttga
     5821 cggcctggcg ccagaagttg ccggagtcct tgttggcggg gccgctggac tcgtcgtaca
     5881 tggcgttggt ggtgaaggcc gccgcgagcc gcgcggtgtt cgcgatgacg gcgttcgacg
     5941 acaccggcga cgcgttcagc ggggtctgtg cgttcaccgg gatgaaggtg tcgcccgaga
     6001 cggcgcggtc cacggagagg ttcgccgcgg cgacggccga tccctcgtcg gccgaggaga
     6061 ccgtcacgag gttctgtccc gggatgcgca gggtcgcgac cttcttgccg tggtcctgga
     6121 tgtcggtgaa cttcagcgtg acgacgttct tcttcacgct caggcgcgcc gtgaggcgca
     6181 cgccgtcgag ggcggtcggg gtgagcacgt attcgaccgt gctcccgtcg atctgggcgg
     6241 agctcaccga gaccggctgc ggagtgccgt tgatctggat cgaggtgagt gcactgtcgt
     6301 tgccgcgcat gacggcgccg ctggctcgat cgatgtactg gacaacctgc gggaaggcgc
     6361 tggaggctcg cacctcgagc acgccggtgc gcagcaccac ctgtccgtcg cccaccgccc
     6421 acgcgggggc cgtactgaag gccgacaggc cggccgcgac gaccgcacag gtcaggccca
     6481 cccccgccca gcgtcgttgc gttctcttca ccctggttct ccttcgaatc ggcgttcggt
     6541 cacgatgatc gcagaagatg tttgcggaaa caaccctgga cttctggcta ttactgcgag
     6601 gaacgtgtcg acaaagcgcc aggaatggat cttctgtaga gcgcaaacac tcggttcggc
     6661 gaaaccgttc ctgaccaggg gcggatgcgc gcattcggcc tcatgcatcg gatgaatgtg
     6721 agggctgagc cgaggcgcgg gggccgaata ggatcgccgg tcaccctaga catccgatca
     6781 ttccgtgcgt cagaatgcca cacgatgcct cattcctcag ggcatcactc cccgatcgat
     6841 gacgctcagg aggcatccgt gctcactact ccgatctccc gccgccaggc gctgcaggtc
     6901 ggcggcttcg ccgccctcgc cgcactgaca cccaccttcc tcgccgcccg gccggcatcc
     6961 gctgccgtgg gcgccccgca ctcggcagca tccgcagccc ggtcgctccc ccgccctcag
     7021 ctctcccgct atgcgctctg gtacggcacg ccggccaagg agtgggagtc acaggcactg
     7081 cccatcggca atgcgcgtct cggcgcgaaa ctcttcggcg accccaccgc cgatgtcgtg
     7141 cagttcaacg agcagagcat gtggggcggc gtgaacgact acgacaacgc cctcgcaggt
     7201 cagggcgacg gcgcctacga cacgtcggtg accgggttcg gttcgtaccg cgacttcggc
     7261 acggtgacgg tcgcgttcgg tggaaccacc accgtgacat cgcccggcgg accctaccgg
     7321 acctcgggtt cggagggcgt ggaccgcagc tacgacggcg actcgcgcac caaatggtgc
     7381 atcgacggcc cgcccgcgca ggtgctctgg caggcagacc tggcggacgc gaccgaggtc
     7441 gcctcgtaca gcctgaccag cgcgaacgac gtccccgccc gcgacccgca ggagtggacg
     7501 ttccagggtt cgtcggacgg atcgtcctgg acgacgctgg acacccggac cggcgcgccc
     7561 ttcgagagcc ggttccagac caagacctac accgtggcga acccggggac ctaccggcac
     7621 taccgcttcg tgttcgcgcc gaaggccggc gtcagccact tccaactgtc cgagatcggc
     7681 ctggcgggcg tggccctcgc cggcggggac ggctggtacg tctcgtctcc gagcgggcac
     7741 gccgattcgc tgctgggcag cgtcgacggc gacgccggca cggtgtggca ggtcggcgac
     7801 gccgggtcgg gggccatctg gcaggtcgag tcagccgcga agcgcgcctt cacggggtac
     7861 accctcacct ccgcagccga tgacgccgcg gccgatccgc gagactggac ggtcgcggga
     7921 tccgacgacg gcctgacctg gacgactctg catcagacca ccggcgagac gttcgccgaa
     7981 cgcggccaga cccgcgcgtt ctcgttcgcc aacacgaagg catacgcggt gtaccgcatc
     8041 gtcttcacgg gtccgtcggc gttccgggtc gccgggatcg ccttcaccgc cggggcgttc
     8101 agcacggcgg gggcgcgcac ggtggtggac tatcgtcggg cgctcgacat cgccgacggc
     8161 acgtacagca cgcacttcgc caccaccggc gggacggtgc tgcgcgaggc gttcgcctcg
     8221 cgtgacgccg acgtcatcgt gctgaggtac tcgaccgatc gcgccggatc gctgaacgcc
     8281 gacatcggca tggtctcggc acaggaggtg aggacgacgg cggatgccgc gaagcggcgc
     8341 ctgtcgttcg cgggcacgat ggcgaacgag ctgagccacg cggccgtgct gcagatcgcg
     8401 gacacggacg gggaggtcgc ggcggcggga gacgggctcc gtgtgacggg ggcgacctcg
     8461 atcaccctgt tgctcgatgc gcgcaccgac tacctgatgg acgccgcgaa gggatggcgg
     8521 ggcgcggcgc ccctgcccgg gatcgagaag gccctggccg cggcatccgc ccgctccttc
     8581 gacgagctgc gctcggcgca cgtcaccgag gtgtcggctc tgatgtcacg cgcgaaggtg
     8641 gactggggca gcacagcgga tgccgtgctg gcgctgccga cccagcgccg gctcacccgc
     8701 tacgccgcgg gcgagtccga cccgacgctg gagcagacca tgtacgccta cgggcgctac
     8761 ctgctgctga gctcgtcgaa gcccggcggg ctgccggcca acctgcaggg gctgtggaac
     8821 aacagcaacc agccggcctg ggccagcgat taccacacca acatcaacat ccagatgaac
     8881 tactggggcg ccgagacggc cgatctgccg gaggcgcacg aggcgctcgc cgcgttcatc
     8941 gagcaggtcg ccgtgccgag ccgcgtcgcg acccggaacg ccttcggtga cgtgcgcggc
     9001 tggacggcgc gcacctcgca gagcatcttc ggcggcaact cctgggagtg gaacaccgtc
     9061 gcgagcgcct ggtacgcgca gcatctctac gagcactggg cgttcacgca ggacaagcgg
     9121 tacctgcgcg atcacgccta cccgctgatc aaggagatct gcgagttctg ggaggaccgc
     9181 ctcaagacgc tcccggacgg cacgctggtg tcgccgaacg ggtggtcgcc cgagcacggg
     9241 ccccgcgagg acggcgtgat gtacgaccag cagatcatct gggacctctt ccagaactac
     9301 ctcgactgcg ccgaagccct cgatgtcgac ccggactacc gggcaacggt ggccgacatg
     9361 cagagcaggc tcgcgccgaa caggatcggc agctggggtc agctgcagga gtggcagacc
     9421 gaccgcgacg atccgaagga catccaccgc cacacatcgc acctgttcgc cgtctacccc
     9481 ggccggcaga tcaccgtgga cgacaccgag ctcgccgcgg ccgcgctggt gtcgttgaag
     9541 gcgcgatgcg gcgagaagga gggcgtgccg ttcaccgccg gcaccgtctc gggcgacagc
     9601 cgccgttcct ggacgtggcc ctggcgcgcc ggcctgttcg cccgcctcgg cgatgcggag
     9661 cgcgcgggga tcatggtgcg cggcctgctc acctacaaca cgctgccgaa cctgtgggcg
     9721 aaccatccgc cgttccagct ggacggcaac ttcggcatca ccggggcgat cgccgagatg
     9781 ctgctgcaga gccacgaggg ggccattcgc ctgctgcccg ccgttccggc ggactgggcg
     9841 gcgaagggct cgttcaccgg cctgcgcgct cgaggcgggt accgggtgag ctgcgaatgg
     9901 caggacggga gggtggtgtc gtacgacgtc gtggcggacc gtgcaccgaa catgtcgaac
     9961 gtcgtcgtca tcgtgaacgg acagcgccgc aaggtcaagc ccgccaaccc caaggccgga
    10021 ctgccgatgc gcgacctcgg cccggcgaac tgagtccggc gaactgagtc gggcgaactg
    10081 agtccggcgg cacggcgtct ccccctcggg cgccgtgccg ccggac
//
LOCUS       DATMSX010000132         6335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_153522,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000132 DATMSX010000000
VERSION     DATMSX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153522"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..2612)
                     /locus_tag="VN041_04015"
     CDS             complement(132..2612)
                     /locus_tag="VN041_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HWU28246.1"
                     /translation="MTLLDPTTVNGADADAVYLAFVEWAESTGIRLYPAQDEAVIEIV
                     SGQNLILSTPTGTGKSLVAVGAHFAALTEGRRSYYTAPIKALVSEKFFSLVEVFGAAN
                     VGMVTGDSAVNADAPIVCCTAEILANLALREGADADVGQVVMDEFHFYGDPDRGWAWQ
                     VPLLTLPQAQFVLMSATLGDVTELASDLTRRTGRETAVVTGVERPVPLHFFYETTPIH
                     ETIEDLLHTDRAPVYIVHFSQAAAMERAQALASAKVASREQRDAIADLIGGFRFTTAF
                     GKTLSRLLRLGIGVHHAGMLPKYRRLVEQLAQRGMLRVICGTDTLGVGINVPIRTVLL
                     TALTKFDGQRMRQLNAREFHQIAGRAGRAGFDTAGTVVAQAPEHETENLAAIRKAGDD
                     PKKKRKIIRKKAPDGFVSWGEPSFQKLISSEPETLTSHMQITSAMMLNVIARGGDVFG
                     NMRALVYDNHEPRAKQRLLALRALGIYRTLLESGVVENGPDGVRLTVDLQPNFALNQP
                     LSPFALAAFELLDPDSATFALDMISIVESTLDDPRAILSQQEFIARGEAVAAMKAEGI
                     EYDERMELLEQITWPKPLEELLAAAFQTFSAAQPWIRDFDLHPKSVVRDMYERAMSFG
                     EYVAFYKTARSEGVVLRYLSDAYRAASQTIPEHLKTEELRDLIEWLGELVRQVDSSLL
                     DEWQELVSGVLVDPDNEPVVPPAPKRLTANVRAFRILVRNELFRRVQLAAREDLESLD
                     AVDPAFDGWSDALDAYFAEHDEILTGADARGSALLLIEQGAEQWTVRQILDDPAGDHD
                     WGISATVDLAASDEAGEAVIHVTGVDRL"
     gene            complement(2647..3468)
                     /locus_tag="VN041_04020"
     CDS             complement(2647..3468)
                     /locus_tag="VN041_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28247.1"
                     /translation="MTAPQNVSAPRDLRRGLLIALSIAAFVLVASTITTGILLFGMLG
                     QRPAAAPEPSASPSPTATREPQTVHGIPVSIRDTALEFGTWVAEPWVDGETRLFAPMS
                     NPSETQAVSIFYDVTAYDAKGRILDRTLGSADLLPGGEGVFSSSSLRAEPDDIAEIVI
                     EQTRISAAASPYTGEISEPQVTADRESGGVDFVIPSSLSPDLDRPWVDVIALVDGEII
                     GQCDAVTAEGARPFTGRCYLQPTAKGVAEVPENHFMTLPENAEVRAFLRVELELE"
     gene            complement(3465..4313)
                     /locus_tag="VN041_04025"
     CDS             complement(3465..4313)
                     /locus_tag="VN041_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28248.1"
                     /translation="MTIPEVPQQAPEPAAPTAAPQRRAGGNGLLVALSILCAVLLLAT
                     LGFGTGAAILSVTNDSAAADPGSTHTPKPTAKVDRDRTVHGIPVDVVSDIDFGYFFMT
                     NTDAKGFTALYSQIRNHDEKRAAIIFFDVSLYDEDAVLLSRWPSSVYMLPGQVTLFTG
                     VTVGNMLDVASIRIEQTSLKYEEPRSTGTVVLDEARSGDNGLVGGKYTSTLGVEGRGA
                     HVYLIAAVDNEVFGVCWDVENIPASGAFDFRCTLEPASRDEPQPIGTLPEDAVVASYY
                     TLDTIQ"
     gene            4490..4879
                     /locus_tag="VN041_04030"
     CDS             4490..4879
                     /locus_tag="VN041_04030"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWU28249.1"
                     /translation="MRRRGAIAVMLAALAAGLVGCASAQTRVDADEPTAVVKVGEEVV
                     VDLGEVNSSVGTEWVLVEEPDPAVLGGEDVDFVSDQPNPPPGSSSNMTVTFDAVGAGE
                     TTLTYEYRFRGEASKDRGPLTFTITVR"
     gene            4968..5546
                     /locus_tag="VN041_04035"
     CDS             4968..5546
                     /locus_tag="VN041_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28250.1"
                     /translation="MTKARILAEARTVFARRGYAEASLREIAEAVGIKTPSLYAHFPS
                     KEALYEAVYAEVVAEHTAFFDELVRTSADLPPLPRLRHLLSGIGAFYRDRPEQHEFSM
                     RAAVDENGPSGQSLREIFLDSESTLAEAVRQSYDDGVTTGAFAGGDPEGFTALVFVVM
                     DGLFLQYAHYSPALYAERFEQTWRHLSALLSA"
     gene            5593..>6335
                     /locus_tag="VN041_04040"
     CDS             5593..>6335
                     /locus_tag="VN041_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWU28251.1"
                     /translation="MPSSRHPVVRITIIDPVVLDGQVLIDPSPEIPGAVIERRSLAFG
                     TASVESRWDDAFAAPGIIDAAIRAEAAGADAVVVNCMEDPAVDAAREVVRIPVVGPAE
                     AGMHLALGVADRFSILTTEAADIPIVREMVERHRLEHRCVSIRAVGLPVLGIGADEND
                     TLRRLTAAAHAAVEDGAAALVLGCTLFASLTDRLAVELPGIPVIDPLSAALHHALTLV
                     RLGVLHSAVGYPAPDAKPISWPAGDVRFGP"
BASE COUNT          936 a         2151 c         2224 g         1024 t
ORIGIN      
        1 ggcgacgttc caccgcgtgc cgccgatcgt gcctgcgacc ctgggcggct gggccggcgc
       61 gaacggctgc gtgctgctgg ggcttcaggc gggctgagcc ccgaagctgc tctggcgcgt
      121 gattcggcgc cttacagccg gtcgacgccc gtgacgtgga tcacggcttc gccggcctcg
      181 tcggatgccg ccaggtcgac ggttgcggag atgccccagt cgtgatcgcc ggcggggtcg
      241 tccaggatct gacgcaccgt ccactgctcc gcgccctgct cgatcagcag gagggcggac
      301 ccgcgggcat ccgcccctgt gaggatctca tcgtgctcgg cgaagtacgc gtcgagggcg
      361 tcggaccagc cgtcgaacgc ggggtccacg gcatccagcg actcgaggtc ctcgcgggcg
      421 gcgagctgca cgcgccggaa cagctcgttg cgcaccagga tgcggaaggc gcgcacgttc
      481 gcggtgagcc gcttgggcgc cggcgggacg accggctcgt tgtccggatc gacgagaacg
      541 ccgctcacca gctcctgcca ctcgtcgagc agactcgagt cgacctggcg caccagctcg
      601 ccgagccact cgatcaggtc gcgcagttcc tcggtcttga ggtgctccgg aatggtctgc
      661 gacgcggcgc gataggcgtc cgagaggtag cgcagcacca cgccctccga tcgcgcggtc
      721 ttgtagaagg cgacgtattc cccgaacgac atggcccgtt cgtacatgtc gcgcaccacc
      781 gacttgggat gcaggtcgaa gtcgcggatc cacggctgcg cggcgctgaa ggtctggaag
      841 gcggcggcga gcagctcctc gagcggcttg ggccacgtga tctgctcgag cagctccatg
      901 cgctcgtcgt actcgatgcc ctccgccttc atcgcggcga cggcctcgcc ccgcgcgatg
      961 aactcctgct gcgagaggat cgcgcgcgga tcgtcgagag tcgactcgac gatcgagatc
     1021 atgtccaggg cgaaggtcgc cgagtccgga tcgagcagtt cgaacgcagc gagggcgaag
     1081 ggcgacagcg gctggttgag agcgaagttc ggctgcaggt cgaccgtgag ccggacgccg
     1141 tccggaccgt tctcgaccac gccggattcg agcagcgtgc ggtagatccc gagggctctc
     1201 agagcgagga ggcgctgctt cgccctgggc tcatgattgt cgtataccag tgcgcgcata
     1261 ttgccgaaca cgtcgccgcc ccgagcgatg acgttgagca tcatggcgct ggtgatctgc
     1321 atgtgcgagg tgagcgtctc aggctcggag gagatcagct tctggaacga cggctctccc
     1381 caggacacga acccgtcggg cgccttcttg cggatgatct tgcgcttctt cttcgggtcg
     1441 tcgccggcct tgcggatcgc ggcgaggttc tcggtctcgt gctccggcgc ctgcgccacg
     1501 accgtgcccg ccgtgtcgaa gccggcgcgg cccgcacgtc ctgcgatctg gtggaactcg
     1561 cgggcgttca gctgccgcat ccgctggccg tcgaacttcg tcagcgcggt gagcagcacg
     1621 gtgcggatgg gcacgttgat gccgacgccc agcgtgtccg tgccgcagat cacccgcagc
     1681 atcccgcgct gggcgagctg ctcgaccaga cgacggtact tcggcagcat ccccgcgtgg
     1741 tgcacgccga tcccgagccg gagcagccgg gagagcgtct tgccgaaggc ggtcgtgaac
     1801 cggaagcctc cgatgaggtc ggcgatcgca tcgcgctgct cgcgcgacgc gaccttcgcg
     1861 ctggcgagcg cctgcgcgcg ctccatcgcg gcggcctggg agaagtgcac gatgtagacg
     1921 ggcgcgcggt cggtgtgcag caggtcctcg atggtctcgt ggatcggcgt cgtctcgtag
     1981 aagaagtgca gcggaacggg ccgctcgacg cccgtgacca cggccgtctc ccgtccggtg
     2041 cggcgggtca gatcagatgc cagctccgtg acgtcgccga gggtcgcgga catgagcacg
     2101 aattgcgcct gaggaagcgt gagcagcggc acctgccatg cccagcccct gtcggggtcg
     2161 ccgtagaagt ggaactcgtc catcacgacc tggccgacgt cggcatccgc accctcgcgc
     2221 aacgcgaggt tcgcgaggat ctcggccgtg cagcagacga tcggtgcatc ggcgttcacc
     2281 gcggagtcgc cggtgaccat gccgacgttc gcggcgccga acacctcgac caggctgaag
     2341 aacttctcgc tgaccagcgc cttgatcggt gccgtgtagt aactgcggcg tccctcggtc
     2401 agcgcggcga agtgggcgcc gaccgcgacc agggacttcc cggtgcccgt gggcgtggac
     2461 aggatcaggt tctgacccga gacgatctcg atgaccgcct cgtcctgcgc ggggtacagg
     2521 cggatgccgg tggactccgc ccactccacg aacgccaggt agacggcgtc cgcatcggcg
     2581 ccgttgacgg tcgtgggatc gaggagggtc acccctcgat cctttcaccc ccgccgcgtc
     2641 accgattcac tcgagctcga gctcgaccct caggaacgcg cgcacctcgg cattctccgg
     2701 cagcgtcatg aagtggttct ccgggacctc ggcgacgccc ttcgcagtcg gctggaggta
     2761 gcaccttccg gtgaacgggc gggctccttc ggcggtgacc gcgtcgcatt ggccgatgat
     2821 ctcgccgtcg acgagggcga tgacgtcgac ccaggggcgg tcgaggtcgg gggagaggga
     2881 cgaagggatg acgaagtcga cgcccccgga ctcacggtcg gcggtcacct gcggctcgct
     2941 gatctcgccc gtgtacggcg atgccgcggc ggagatcctg gtctgctcga tcacgatctc
     3001 cgcgatgtcg tcgggttcgg ccctcagact cgacgacgag aagactccct cgccgcccgg
     3061 caggaggtcg gcactgccga gcgtgcggtc gaggatgcgt cccttcgcgt cgtatgccgt
     3121 gacgtcgtag aagatcgaca cggcctgcgt ctcggagggg ttgctcatcg gcgcgaacag
     3181 ccgcgtctcg ccgtcgaccc acggctccgc cacccaggtg ccgaactcga gcgccgtgtc
     3241 acggatgctg acggggatgc cgtgcaccgt ctgcggttcg cgtgtcgcgg tcggcgacgg
     3301 tgaggcggat ggctctggtg ccgccgccgg ccgctgcccc agcatcccga acagcaggat
     3361 gccggtggtg atcgtcgagg cgaccagcac gaacgctgcg atgctcagtg cgatgaggag
     3421 gccgcgccgg aggtcgcggg gtgctgagac gttctgcggc gccgtcattg gatcgtgtcc
     3481 agggtgtagt agctggcgac cacggcgtcc tccgggagcg tgccgatcgg ctgcggctcg
     3541 tcacgtgagg cgggttcgag tgtgcagcgg aagtcgaacg cgccggaggc ggggatgttc
     3601 tcgacgtccc agcagacgcc gaagacctcg ttgtcgacgg cggcgatcag gtacacgtgc
     3661 gcccctcggc cctcgacgcc gagtgtcgag gtgtacttgc cgccgaccag gccgttgtcg
     3721 ccgctgcggg cctcgtcgag gaccacggtg cccgtgctcc gcggctcctc gtacttcagg
     3781 ctggtctgct cgatcctgat cgaggcgacg tccagcatgt tcccgacggt cacgcccgtg
     3841 aagagcgtca cctgtcccgg aagcatgtag acgctcgagg gccagcggct gagaagcacg
     3901 gcatcctcgt catacaggct cacatcgaag aagatgatgg ccgcgcgctt ctcgtcgtgg
     3961 ttgcggatct gcgagtacag cgccgtgaag cccttggcat ccgtgttggt catgaagaag
     4021 tagccgaagt cgatgtcgga cacgacatcg accgggatgc cgtgcaccgt gcggtcccgg
     4081 tcgaccttcg cggtcggctt gggggtgtgc gtcgagccgg ggtccgctgc ggcgctgtcg
     4141 ttggtcacgg acaggatggc cgcgccggtg ccgaagccga gcgtcgcgag cagcagcacg
     4201 gcgcagagga tggacagcgc caccagcagg ccgttcccgc ccgccctgcg ctgtggcgcg
     4261 gctgtgggtg ccgcgggctc gggcgcctgc tgcgggacct ccggtatcgt catcgactcg
     4321 cctcctctgg atcggagcat agcggcggcg tggcgccgac gccgatgggg tgttgtcccc
     4381 acgtgttgcg ttcgtctgca cgcggcgcgg cagtggggcg cagcggcgtg agcacggcat
     4441 cctcgcagga gcagggcgac gacgagcacg ccgcccgaga gggggacgga tgcggagacg
     4501 tggcgcgatt gcggtgatgc tcgccgccct ggccgcgggc ctcgtgggct gtgcgtccgc
     4561 tcagacgagg gtggacgccg acgaacccac cgccgtggtg aaggtgggcg aagaggtcgt
     4621 cgtggatctc ggtgaggtga actcctccgt cggcacggag tgggtgctcg tcgaggaacc
     4681 ggatccggct gtgctgggtg gtgaggacgt cgacttcgtc tccgatcagc cgaatccgcc
     4741 gccgggctcg agctcgaaca tgacggtcac cttcgatgcg gtcggcgccg gggagaccac
     4801 cctgacctac gagtaccgct tccgcggtga ggcgtcgaag gacaggggtc cgctgacctt
     4861 cacgatcacg gtgcgctgaa gcgccgtctg aaacagatct gggcgatggt tgcgcgcagg
     4921 gccggtcgcg cctattctct tcctaactaa tgatcgttag gtacgacatg accaaagcac
     4981 gcatcctcgc cgaagcccgg acggtcttcg cgcgcagggg gtatgccgag gccagtcttc
     5041 gtgagatcgc cgaggccgtc ggaatcaaga cgccctccct gtacgcgcac ttccccagca
     5101 aggaggcgct gtacgaggcg gtctacgccg aggtcgtcgc agagcacacc gcgttcttcg
     5161 acgagctcgt ccgcaccagc gcggatctcc cgcccctgcc gcgactgcgc catctgctca
     5221 gcggcatcgg ggccttctat cgcgatcgcc ccgagcagca cgagttcagc atgcgcgccg
     5281 ccgtcgacga gaacgggccc agcgggcaga gcctccggga gatcttcctc gactccgagt
     5341 cgaccctcgc cgaagccgtc aggcagtcgt acgacgacgg cgtgacgacg ggagcgttcg
     5401 cagggggcga ccccgagggc ttcaccgcgc tggtgttcgt cgtcatggac ggcctgttcc
     5461 tgcagtacgc ccactacagc cccgcgctgt acgcggagcg cttcgagcag acctggcggc
     5521 acctctccgc acttctctcc gcctgacccg ccgcatcgca gcgacgggac ggcacccccg
     5581 aaagcctgtg ccatgccctc ttcgcgccac cccgtcgtgc gcatcacgat catcgacccc
     5641 gtcgtcctcg acggccaggt gctcatcgac ccgtctcccg agatccccgg cgccgtcatc
     5701 gagcgccgca gcctcgcctt cggcacggca tccgtcgaga gccgctggga cgacgcgttc
     5761 gcggcgcccg ggatcatcga cgccgctatc cgagcggaag ccgcgggcgc ggatgccgtg
     5821 gtcgtcaatt gcatggagga tccggcggtc gacgccgccc gcgaggtcgt gcggataccc
     5881 gtggtggggc cggcggaggc cggcatgcac ctggctctgg gcgtggcgga ccggttcagc
     5941 atcctcacca ccgaggccgc cgacatcccg atcgtgcggg agatggtcga gcgccaccgg
     6001 ctcgagcacc gctgcgtctc gatccgcgcg gtcggcctgc ccgtcctggg catcggagcg
     6061 gatgagaacg acacgctccg ccggctgacc gcggccgcac acgcggcagt cgaggacggg
     6121 gccgccgctc tcgtgctcgg ctgcaccctg ttcgcgagcc tgaccgaccg cctcgccgtc
     6181 gagctcccgg gcatccccgt gatcgacccg ctctccgccg ccctgcacca cgccctcacg
     6241 ctggtgcgcc tcggagtctt gcactccgcc gtcggctacc cggccccgga cgccaagccc
     6301 atcagctggc ccgcgggcga cgtgcgcttc ggacc
//
LOCUS       DATMSX010000133         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_11276,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000133 DATMSX010000000
VERSION     DATMSX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11276"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1258)
                     /gene="thrB"
                     /locus_tag="VN041_04045"
     CDS             complement(296..1258)
                     /gene="thrB"
                     /locus_tag="VN041_04045"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWU28252.1"
                     /translation="MEPVVSEAHAVEGALRTVIVTVPATSANLGPGFDTLGLALSIYD
                     TLTASSFDDDRLEIEVEGSGAAEIPTDASNLVVRTIAHVYADAGRRMPGLRIHALNGV
                     PHGRGLGSSGAAVTAGILIAKGLLAGDVDLDDEALLRLATELEGHPDNVAPALFGGLT
                     IAWMGERGPQHKKLLVHRGVAPLVFVPGFTMSTSLARSLQPPHVPREDAVFNVSRSAL
                     LVAALTQSPELMLDATADRLHQDYRGAAMPETLRLVQALRAEGFAAVVSGAGPSVLVL
                     ADGPGTRQRAVQVADAVTDTPWDAHMLAVDVRGGTVRDRAEGST"
     gene            complement(1288..2604)
                     /locus_tag="VN041_04050"
     CDS             complement(1288..2604)
                     /locus_tag="VN041_04050"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWU28253.1"
                     /translation="MTDYRRLRVALLGAGAVGSQVASLLLKHGDELADRAGARLELAG
                     IAVRNPDAPRDVELPRELYTTDPEALITSADIVIELMGGIEPARSHILLGLGSGADVV
                     TANKALLATHGPEVFEAADRVGASVYYEAAAAGAIPIIRPLRDSLAGDRVVRIMGIVN
                     GTTNYILDRMDSEGADFADVLADAQRLGYAEADPTADVEGYDAAQKAAILASLAFHTA
                     VPLDAVHREGITTITADMIEEAREAGFVIKLLAVCERLTTEEGESISVRVYPALVPRS
                     HPLASVHGANNAVFVEAEAAGSLMFYGAGAGGMQTASAVLGDVVSAARRHMAGGTGVG
                     ESTRANLPIVSIGHVVTRYQITLEVADQPGVLASIAGLLSEGGVSVATLVQTAIPAIE
                     GEQRSTARLIIGTHRAADSALSAAVARLTASDVVEHVVSVLRVEGE"
     gene            complement(2675..4066)
                     /gene="lysA"
                     /locus_tag="VN041_04055"
     CDS             complement(2675..4066)
                     /gene="lysA"
                     /locus_tag="VN041_04055"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWU28254.1"
                     /translation="MHSAVSSPAPEWLVVPEDANDLVPAIWPASAHRDADGELVVGGV
                     PAGLLARTYGTPLQVLDEGEVRRRAAAIRSAFDAAAEAHGTTARVYYAGKALLNTSVV
                     RWVTEEGLNVDVCTGGELEVALAAGADAGRLGFHGNNKSVPELERAVEVGIGTVVIDS
                     EIEIERLAAIVSRTGTTQRVMLRVNSGVHAETHDFLATAHEDQKFGTPLSLAPGLVSR
                     IREIEGLEFVGLHCHIGSQIFGVAGFRESAARVLDLHQELLAGGDVPQLNLGGGFGIA
                     YTRVDTPEPIEDLAQGIVDAVAEGCAARGIRIPALSFEPGRAIVGNAGITLYEVGTIK
                     DVALEQGERRYVSVDGGMSDNARPALYGAQYSARLASRAGEDAPVLVRVVGKHCESGD
                     IVVDHEYLPGDVAPGDLLAVPSTGAYCAPLSSNYNHIARPPIVAVRDGESRVIVRGES
                     LDDLLARDAGIER"
     gene            4184..>5193
                     /locus_tag="VN041_04060"
     CDS             4184..>5193
                     /locus_tag="VN041_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWU28255.1"
                     /translation="MSRRTDTSRPGAFARASAAVIARALRWRLVRAALRYSGRRGPVL
                     ADAVTYRALFSVFAAVLLAFSAAALWLSRDDAAWDAVVHAVDTAVPGLIRTSGGQGIV
                     DLDEIRAPAGLSITGIVSLAGLIGAALGAISSLRISVRSMAGTARDDVAWYLVIARNV
                     LLALIIAVTFAGAAALTLAGEFVVDWLAGILGLPPKSAAVLWTVRLVSLVIVLALNAV
                     LIAATFRMLSGVPASARSLWSGAVLGGMALLVLQELSGLFVGGAKANPLLASFASLLA
                     LLLWLNLSTQVILLASAFIVVSVHEEHDRVAARFGATTLADAAVRLAEEDVRTATEAL
                     RTA"
BASE COUNT          704 a         1826 c         1876 g          787 t
ORIGIN      
        1 gcatcagccg cggcagcggc ggatgccgtg gtggcgcgac gcggggcgcg cttgcgcacc
       61 ggagcagcct cggaagccgg cgcctcagcg gtcggctggt cgttctgggt ctcggagaag
      121 gtctccacga gtactccctc atatgtcgtg ggaaatgagc aatccagatg tgcgcagcag
      181 aagctgcgcg tttcacacgc gccgacgcag aagagcgtct gcctgccaga acctggatgg
      241 tgctggggat cgcgtgcgga tttcgcagaa gtgcgatggg gcgagattca cgaagttacg
      301 tggagccctc cgctcgatcc ctaactgtac caccacgcac gtcgacggcg agcatgtgcg
      361 cgtcccacgg agtgtccgtg acggcatccg ccacctggac cgcgcgctgg cgcgtgcctg
      421 gtccgtccgc gagcaccagc acgctcggcc ccgcgccgga cacgacggct gcgaacccct
      481 cggctcgcag cgcctgcacc agtcgcaggg tctccggcat agcggcgccg cggtagtcct
      541 ggtgcagcct gtcggcggtg gcgtccagca tcagctccgg gctctgcgtc agcgccgcca
      601 ccagcagcgc cgagcgcgag acgttgaaca cggcgtcctc gcgcggaacg tgcggcggct
      661 gcagcgagcg ggcgagcgag gtcgacatgg tgaacccggg cacgaacacc agcggcgcga
      721 cgccgcggtg caccagcagc ttcttgtgct gcggtccgcg ctcgcccatc caggcgatcg
      781 tcagaccacc gaagagcgcc ggagcgacgt tgtcggggtg cccctccagc tcggtggcca
      841 ggcgcagcag cgcctcgtcg tcgaggtcga cgtctcccgc cagcagaccc ttggcgatca
      901 ggatgccggc ggtgaccgcc gcaccggatg accccaggcc gcgcccgtgc ggcacgccgt
      961 tgagggcgtg gatgcgcaga ccgggcatcc gtcgcccggc atcggcgtag acgtgtgcga
     1021 tggtgcggac cacgaggttg ctcgcgtccg tggggatctc ggcggcgccc gagccctcga
     1081 cctcgatctc caggcggtcg tcgtcgaagg acgacgcggt gagggtgtca tagatgctga
     1141 gcgcgaggcc cagggtgtcg aagccggggc cgaggttcgc gctggtggca ggcacggtca
     1201 cgatgaccgt gcgcagcgct ccctcgacgg cgtgcgcctc ggagacgacg ggctccatgt
     1261 ccgccggcac gagggtgacg gtcatgctca ctccccttcg acgcgcagca ccgagacgac
     1321 gtgctcgacg acgtcgctgg ccgtgagccg cgcgacggcc gcgctcagcg cgctgtcggc
     1381 ggcgcggtgc gttccgatga tcaggcgcgc ggtgctgcgc tgctcaccct cgatcgcagg
     1441 gatcgcggtc tgcacgagcg tcgccaccga cacgccgccc tcgctgagga gaccggcgat
     1501 cgaggccagc acgcccggct ggtcggcgac ctcgagcgtg atctggtagc gggtcacgac
     1561 gtggccgatc gagacgatcg gcagattcgc cctggtcgac tcgcccacac ccgtgccgcc
     1621 ggccatgtga cgccgggcgg cggagaccac gtcgccgagc acggcagatg cggtctgcat
     1681 cccgcccgct cccgcgccgt agaacatcag ggagccggcc gcctcggcct cgacgaagac
     1741 ggcgttgttc gcgccgtgca cggaggcgag cggatgcgag cgcggcacga gtgcgggata
     1801 cacgcgcacc gagatcgact caccctcctc cgtggtcagc cgctcgcaga cggcgagcag
     1861 cttgatcacg aagccggcct cacgggcctc ctcgatcatg tcggcggtga tggtcgtgat
     1921 gccctcgcgg tgcacggcgt cgagcgggac ggcggtgtgg aaggccaggg atgccaggat
     1981 cgccgccttc tgcgcggcgt cgtagccctc gacgtcagcc gtcgggtcag cctcggcgta
     2041 gcccagccgc tgcgcgtcgg cgagcacgtc ggcgaagtcg gcgccctcgc tgtccatgcg
     2101 gtcgaggatg tagttcgtgg tgccgttgac gatgcccatg atccgcacca cgcggtcgcc
     2161 ggcgagggag tcgcgcagcg gccggatgat cgggatcgcg ccggcggcgg ccgcctcgta
     2221 gtacaccgag gcgccgaccc ggtcggctgc ctcgaagacc tcggggccgt gagtggccag
     2281 cagcgccttg ttcgcggtga ccacgtcggc gcccgagccc aggcccagga ggatgtgcga
     2341 acgtgccggc tcgatgccgc ccatcagctc gatcacgatg tcggcgctcg tgatcagcgc
     2401 ctccggatcg gtcgtgtaca gctcgcgagg cagctcaacg tcgcgcggcg cgtcggggtt
     2461 gcgcaccgcg atgcctgcca gctccagccg cgcgcccgcg cggtcggcga gctcgtcgcc
     2521 gtgcttgagc agcagggacg ccacctgcga gcccacggct ccggcaccca gcagtgccac
     2581 acgaagtcgt cggtaatcag tcatcagcgc tcctcagcac atcggggtcg tcgggtgtcg
     2641 ggcgcggaat gcgcgcccga cgtctgcagg ggcttcaccg ctcgatgccg gcgtcgcggg
     2701 cgagcaggtc gtccaggctc tcgccgcgca cgatcacccg ggactccccg tcgcgcacgg
     2761 cgacgatcgg cgggcgggcg atgtggttgt agttgctcga cagcggcgcg cagtacgccc
     2821 ctgtcgaggg cacggccagc aggtcaccgg gggcgacgtc gccgggaagg tactcgtggt
     2881 cgacgacgat gtctccggac tcgcagtgct tgccgaccac ccgcacgagc accggggcgt
     2941 cctcgccggc gcgcgacgcc agacgcgccg agtactgcgc accgtacagc gcggggcggg
     3001 cgttgtcgct catgccgccg tccacgctca cgtagcgccg ctccccctgc tcgagggcga
     3061 cgtccttgat cgtgcccacc tcgtagaggg tgatgccggc gttgccgacg atggcccggc
     3121 cgggctcgaa cgacagggcg gggatgcgga tgccgcgcgc cgcgcagcct tccgccaccg
     3181 cgtccacgat gccctgcgcg agatcctcga tgggctcagg cgtgtcgacc cgggtgtagg
     3241 cgatgccgaa gccgccgccg aggttcagct gcgggacgtc accgcccgcc agcagctcct
     3301 ggtgcaggtc gagaacgcgt gcggctgatt cgcggaagcc ggcgactccg aagatctgag
     3361 acccgatgtg gcagtgcagg ccgacgaact cgaggccctc gatctcgcgg atgcgggaca
     3421 cgagcccggg ggcgagggac agcggggtcc cgaacttctg atcctcgtgc gccgtcgcca
     3481 ggaagtcgtg cgtctccgcg tggaccccgc tgttgacgcg cagcatgacg cgctgcgtgg
     3541 tcccggtgcg ggagacgatg gcggcgagcc gttcgatctc gatctcgctg tcgatgacga
     3601 cggtgccgat gccgacctcg accgcccgct ccagctcggg cacggacttg ttgttgccgt
     3661 ggaagcccag tcgtccggca tccgctccgg ctgccagcgc cacctcgagc tcaccaccgg
     3721 tgcagacgtc gacgttcagt ccctcctccg tcacccaccg cacgacggag gtgttcagca
     3781 gggccttgcc cgcgtagtac acccgcgcag tggtgccgtg cgcctcggcc gcggcgtcga
     3841 aggcggagcg gatggctgca gcgcgacgcc gcacctcgcc ctcgtcgagc acctgcagcg
     3901 gcgtgccgta cgtgcgggcc agcagcccgg ccggcactcc cccgacgacc agctcgccgt
     3961 ccgcgtcgcg gtgggcggac gccggccaga tcgcgggcac gaggtcgttg gcgtcctcgg
     4021 ggacgaccag ccattcggga gcgggcgatg agacagctga atgcacagag caccaatcgt
     4081 gggcgggacc tcgggcgggg agcgcacgac gaggctgccc cgattctagg tcatctccgc
     4141 aaccccctcg gcctgtgttc cctggcatga ctatggtggc cggatgagcc gacgaaccga
     4201 tacctcgcgt cccggcgcct tcgccagggc gtccgccgcg gtcatcgccc gagcgctgcg
     4261 ctggcggctg gtccgggccg ccctgcgcta ttcagggcgc cggggcccgg tgctcgcgga
     4321 cgcggtgacc taccgcgccc tgttcagtgt gttcgccgcc gtgctgctcg ccttctcggc
     4381 cgctgcgctg tggctctcgc gggatgacgc cgcgtgggat gcggtcgtgc acgcggtcga
     4441 caccgcggtg ccgggtctga tccgcacctc cggtggccag ggcatcgtcg atctcgacga
     4501 gatccgcgct cctgccgggc tgtcgatcac cgggatcgtc tcgctcgccg gcctcatcgg
     4561 cgcggcgctc ggcgcgatca gctcgctgcg catctcggtc aggtcgatgg ccggcacagc
     4621 gcgcgacgac gtcgcctggt atctggtgat cgcgcgcaac gtcctgctcg cgctgatcat
     4681 cgcggtgacg ttcgccggcg ccgccgccct gacgctggcg ggcgagttcg tggtcgactg
     4741 gctcgcgggc atcctcggtc ttcccccgaa gtccgcagcc gtgctctgga cggtgcggct
     4801 cgtctcgctc gtcatcgtgc tcgcgctcaa tgcggtgctc atcgccgcga ccttccggat
     4861 gctgtcgggc gtgccggcct cggcacgatc gctctggtcc ggcgccgtgc tcggcggaat
     4921 ggcgctgctg gtgctgcagg agctgtcggg gctgttcgtc ggcggcgcca aggccaatcc
     4981 gctgctcgcg tcgttcgcgt cgctgctggc gctgctgctg tggctgaatc tgtcgacgca
     5041 ggtgatcctg ctggcgagcg cgttcatcgt ggtgtcggtg cacgaggagc acgaccgcgt
     5101 ggcggccagg ttcggagcca cgacgctcgc cgacgccgcg gtgcgactcg ccgaggagga
     5161 cgtgcgcacc gcaaccgagg cgctgcggac ggc
//
LOCUS       DATMSX010000134         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_11283,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000134 DATMSX010000000
VERSION     DATMSX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11283"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VN041_04065"
     CDS             complement(<1..267)
                     /locus_tag="VN041_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU28256.1"
                     /translation="MRIDAQLAATVAAVVDEGSFEAAARRLHITQSAVSQRIKALEVQ
                     IGRAVLVRSRPVRATEAGEALVRLARQVELLEHDTVAAFGLGDAD"
     gene            338..1003
                     /locus_tag="VN041_04070"
     CDS             338..1003
                     /locus_tag="VN041_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HWU28257.1"
                     /translation="MLTLLSGLGLMFSLIIAVGAQNVFVLRQGLRREHVLPVVLICAA
                     SDALLVVAGTAGLGYLIGQLPWLLVAARWLGGISLVVYGIYAARRAWGSNGESLVADA
                     SPAADAEGGSRTGGTATATRSRLATVVIATLAFTYLNPNVYLDTVLLIGSIAATHGDQ
                     RWLFAAGAILASITWFFALGFGARHLGRWLRTPRAWRILDTGVAVLLVVMGVLLVLPV
                     FTG"
     gene            complement(1004..1363)
                     /locus_tag="VN041_04075"
     CDS             complement(1004..1363)
                     /locus_tag="VN041_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWU28258.1"
                     /translation="MGAVDEYLSTVTDEADLASLARIYAIAQDLAPDAEQGTGYRMPA
                     LKLRGKALISAIRTKTHIGVYPFSSAAVAAVAGAVAGIPGVEASTGAIRIDPGSEPPE
                     ALIRDLILFRISEIEGR"
     gene            complement(1448..2137)
                     /gene="rplA"
                     /locus_tag="VN041_04080"
     CDS             complement(1448..2137)
                     /gene="rplA"
                     /locus_tag="VN041_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWU28259.1"
                     /translation="MATKSKAYQAALAKIEADRFYTPTEAVALAKETGSAKFDSTVEV
                     ALKLSVDPRKADQMVRGTVMLPHGTGKTARVIVFANGPAAEAAIAAGADEVGGTELIE
                     KVAAGWTDFDAAVSTPELMGQVGRLGKVLGPRGLMPNPKTGTVTPNPAKAVEEIKGGK
                     IEFRVDKHANVHFIVGKASFTAEQLNENIGAALEEIVRLKPSSSKGRYIQKGAVSTTF
                     GPGIPLDVNAI"
     gene            complement(2149..2580)
                     /gene="rplK"
                     /locus_tag="VN041_04085"
     CDS             complement(2149..2580)
                     /gene="rplK"
                     /locus_tag="VN041_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWU28260.1"
                     /translation="MAPKKKVTGLIKLQINAGAANPAPPIGPALGQHGVNIMEFCKAY
                     NAATESQRGNVIPVEITVYEDRSFTFILKTPPAAELIKKAAGVPKGSATPHTVKVAKI
                     TMDQVRAIAEQKQADLNANDIDAASKIIAGTARSMGITVEG"
BASE COUNT          346 a          894 c          873 g          480 t
ORIGIN      
        1 atccgcgtcg ccgagcccga acgcggcgac cgtgtcgtgc tcgagcagct cgacctgcct
       61 ggccaggcgc acgagcgcct caccagcctc ggtcgcacgc accggacggg agcgcaccag
      121 cacggcacga ccgatctgca cctccagcgc cttgatccgc tggctcaccg cggactgcgt
      181 gatgtgcaac cggcgggcag ccgcctcgaa gctgccctcg tccacgacag cggccacggt
      241 cgccgcgagc tgagcatcga tcctcatatc agtcatgcta atggtggagc agttctcttc
      301 gctggtgctg atagccccgt acacctaacg tggagtcgtg ctcaccctgc tctccggcct
      361 cggcctcatg ttctcgctca tcatcgccgt cggcgcccag aacgtgttcg tgctgcgcca
      421 gggtctgcgc cgcgagcacg tgctgcccgt cgtgctgatc tgcgcggcat ccgatgccct
      481 gctggtggtc gccggaaccg ccggcctggg ctatctgatc ggccagctcc cctggctgct
      541 cgtcgccgcg cgctggctgg gcggcatctc gctggtcgtc tacgggatct acgccgcgcg
      601 ccgcgcctgg ggctcgaacg gcgagtcgct cgtcgcggat gcctcgcccg cagcggatgc
      661 tgagggcgga tcgcgcacag ggggcacggc cacggccacg cgatcacgcc tggcgacggt
      721 ggtcatcgcg acgttggcct tcacgtacct gaaccccaac gtctacctcg acaccgtgct
      781 gctgatcggc tcgatcgccg ccacgcacgg cgatcagcgc tggctgttcg ccgccggcgc
      841 gatcctggcg agcatcacct ggttcttcgc gctcggcttc ggcgcccggc acctggggcg
      901 ctggctgcgc accccgcgcg cctggcgcat cctcgacacc ggcgtcgccg tgctgctggt
      961 ggtcatgggc gtgctgctgg tgctccccgt gttcaccggc tgatcagcga ccctctatct
     1021 ccgagatccg gaagaggatc agatcgcgga tgagggcttc gggaggttcc gagccggggt
     1081 cgatgcggat cgcacccgtg ctggcctcga ccccgggaat cccggcgacc gctccggcca
     1141 cggcggcgac ggcggccgag ctgaagggat agacgccgat gtgggtcttc gtgcggatcg
     1201 cggagatcag cgccttgccg cgaagcttga gcgcgggcat ccggtacccc gtgccctgct
     1261 cggcatccgg cgccaggtcc tgcgctatcg cgtagatccg cgcgagcgac gcaagatccg
     1321 cctcgtcggt caccgtgctc aggtactcgt cgacagctcc catgcgctca tcctggcgtc
     1381 cgccggacgc acgcagaagg cccccgcccg gcgaaccggg cgggggcctt catgaagacg
     1441 atcagcctca gatggcgttg acgtccagcg ggatgccggg gccgaacgtg gtcgacacgg
     1501 cgcccttctg gatgtaacgg cccttcgagc tcgacggctt cagacgcacg atctcctcga
     1561 gagcggcgcc gatgttctcg ttcagctgct cggcggtgaa ggaggccttg ccgacgatga
     1621 agtgcacgtt ggcgtgcttg tcgacgcgga actcgatctt gccgcccttg atctcctcca
     1681 cggccttggc cgggttgggg gtcacggtgc cggtcttcgg gttcggcatc aggccgcgcg
     1741 ggccgagcac cttacccaga cgaccgacct ggcccatgag ctcgggggtc gacacggctg
     1801 cgtcgaagtc ggtccagcct gcggcgacct tctcgatcag ctcggtgcca ccgacctcgt
     1861 cggcgcccgc ggcgatggcg gcctcagcag ccgggccgtt ggcgaacacg atgacgcggg
     1921 cggtcttgcc ggtgccgtgc gggagcatga cggtgccgcg caccatctgg tcggccttgc
     1981 gcgggtcgac ggagagcttc agcgcgacct cgacggtcga gtcgaacttc gccgaaccgg
     2041 tctccttcgc gagagccacg gcctcggtcg gagtgtagaa acggtctgcc tcgatcttcg
     2101 cgagggcagc ctggtatgcc ttggacttgg tagccattgt tatctccctc agccctcgac
     2161 cgtgatgccc atggagcggg cggttccggc gatgatcttc gacgcggcgt cgatgtcgtt
     2221 cgcgttcagg tcggcctgct tctgctcggc gatcgcacgg acctggtcca tcgtgatctt
     2281 cgcgaccttg acggtgtgcg gggtcgcgga gcccttgggg acgcctgcgg ccttcttgat
     2341 cagctcagct gccggcgggg tcttcaggat gaacgtgaag ctgcggtcct cgtagacggt
     2401 gatctccacg gggatgacgt tgccgcgctg cgactcggtc gcggcgttgt acgccttgca
     2461 gaactccatg atgttgacgc catgctgacc gagcgcgggg ccgatcggcg gcgccgggtt
     2521 ggctgcaccg gcgttgatct gaagcttgat caggccggtc accttcttct tcggtgccat
     2581 tctctttcct ttc
//
LOCUS       DATMSX010000135         7680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_153539,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000135 DATMSX010000000
VERSION     DATMSX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153539"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN041_04090"
     CDS             complement(<1..216)
                     /locus_tag="VN041_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU28261.1"
                     /translation="MIASSYAPHIGGVEEHVAQVSRALRSQGHEVEVWSVDRGRRPTA
                     SALETPPVRYLPTPLPARTAAALLRYAL"
     gene            complement(242..1810)
                     /locus_tag="VN041_04095"
     CDS             complement(242..1810)
                     /locus_tag="VN041_04095"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWU28262.1"
                     /translation="MRLRDYGNAFRRYWLAILLMIAVGAGAGFGWAKLQTPVYQADAS
                     GYITAGSKFDTSIFSPGQVDGLAKAKVPTYLDMATWRSVAERAGEEIGSKLSPEALAR
                     LITVENPQGTAILKISARAATPEAARDIASAWVTALAAEIDRVEGDGNPGSAELTINL
                     GESASLPSSPVFPDTRLAMIVGIVLGLGGGIAFALVRAMSDRRLRTAADVEQRLHIPV
                     AGTLPDVGSLSDGHRLYRPGDVSEDARAQRADFAVRESLRMLRTNLQFMNVDNPPRRI
                     VVTSALPGEGKSTVAANLAGTLAANGAHVILVDGDLRRPTVAESTGVDGAVGLTDVLA
                     GRLEITDALRRVQGVPNLLVLPAGTLPPNPSELAGSERMHKLLADLAKHAIVIIDAPP
                     LLAVTDGAVLTRAADGALIVVSAGHTSYDTVDDAVGVLEKIHGHVLGVVLNRVAVPNA
                     GAYGAYAYDAKQPRNSDAAPGRAEDKPAATPAASAEAVTAPEPGRRRGARKSTDEDVM
                     TELFGRTPADDSGR"
     gene            complement(1836..3239)
                     /locus_tag="VN041_04100"
     CDS             complement(1836..3239)
                     /locus_tag="VN041_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU28263.1"
                     /translation="MGTGTVRSRLGEFWADAWRWCLFALGVVFAVYLLLDRGIPNGAV
                     AASVVAALTVGVVLTASEPLAIALMATPALLVTERLGLGGADLTVSDAALAAALGAAV
                     LLGKHAYSPPMRVMLWANLGYQFASLLTVIVNPYVQNTVEWVHAWLLVSGALIVGWVI
                     GRAGRARLAFILILGVAGVIAAGTFGTAIGHALHGEFGAVYPNWPWPMHKNFAGGMLG
                     FAAFIAYVNPDWARIDRRWTRAAFVVFLIALLLTQSRQAGIGLALALLVHTLRRGAAK
                     HIVLIIVLAIPGIYLVVQSVFEQIDSQNRFNSFYQRVDWLREVYAFWKHSPVFGHGLR
                     YWYVQPGANFQPPQAEVEVLASTGIVGLIGFIAMWAVILIALWRVAPRFGMLAFGLVL
                     ARIAQAQFDLFWVAAQVSVPFFVAGICLGAQALAHEHGPTQAFWAIREKKSRRDRDLD
                     LPERVLVRSGVRRTQHI"
     gene            3489..4130
                     /locus_tag="VN041_04105"
     CDS             3489..4130
                     /locus_tag="VN041_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28264.1"
                     /translation="MTIENTNVSRRTLVKGAAWSLPVVAVAAATPLAAASTTNASLAW
                     TGTNTGLLQLQLLDGTGVLGAQVAITVPDEYTITNGAGAITAENATVTITVGRPSGIP
                     ISVGRARGFGVASVDGIATTSGERTTTYQTVPVVGTEFGFPITTWSGTRSVTVASNGT
                     VAVPVVFGLAGQSDLLSITALASFPVNLTVAFADGSSYTASSAISVPVGAGVL"
     gene            4252..5493
                     /locus_tag="VN041_04110"
     CDS             4252..5493
                     /locus_tag="VN041_04110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWU28265.1"
                     /translation="MRLSVFGCGYLGAVHAAAMASIGHEVVGVDVDPRKIESLAKGEA
                     PFFEPGLQQLLSEGIASGRLSFTTDASAAHGAKVHFVGVGTPQQAGGHAADLTYVNTA
                     IDDLLPHLSAGDIVAGKSTVPVGTAAELAPRIAAAGAVLVWNPEFLREGWAVQDTIDP
                     DRLVAGVPSGEEGQRAAEVLREVYHPAVAKGTPFIVTDYATAELVKVSANAFLATKIS
                     FINAMAEIAEATGADVTQLADAIGHDARIGRKFLGAGIGFGGGCLPKDIRAFAARAEE
                     LGRGESVNFLREVDAINLRRRERAVQLVVDGLGGSVYGKKVAVLGAAFKPHSDDVRDS
                     PALDVAVRLNGLGADVVVTDPQAIENARRLHPQLTFEADRDETIRGAAAVVMVTEWDE
                     YAGRDRPGEPVRRSPRVAAAR"
     gene            5429..5788
                     /locus_tag="VN041_04115"
                     /pseudo
     CDS             5429..5788
                     /locus_tag="VN041_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5814..6572)
                     /locus_tag="VN041_04120"
     CDS             complement(5814..6572)
                     /locus_tag="VN041_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWU28266.1"
                     /translation="MGDDLTLGLTLSGGGAYGAAQAGVLQVLEERGIRPGIVAGTSAG
                     ALIGAAYAARLDADAIESAVRRFRWSHIARWSARPRWGVLDTHAVTDAVHRILGEDPM
                     IEDLPRRFGAYATDLRTRRGVILDHGPLSSALRATIAVPGLLPPVRRSGRLLADGGMI
                     DNVPDGVTRALGATRTIIVRLHAKWENVRMMRTTAATADLVADPTVILIQPDMVRMAQ
                     WMTKDVPRLIAEGRRAAEEGLDAAIREGRLLAAA"
     gene            6821..>7680
                     /gene="tyrS"
                     /locus_tag="VN041_04125"
     CDS             6821..>7680
                     /gene="tyrS"
                     /locus_tag="VN041_04125"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWU28267.1"
                     /translation="MTRADDILRPMLRTIDAIVDEHALRAKLESGRQLRIKYGVDLTA
                     PDLHLGHAVNLWLMRHLQDNGHRVVFLLGDLTTRVGDPTGKSETRPVLTAEQIEQNAQ
                     AFLDQVSIVLDTAPEVFEVRRNSEWFDRMPVAELLSLFSMTTHAQLMARDMFRERLAQ
                     GREIALHELTYPVLQGYDSFAMQSDLTIVGSDQLFNEMMGRQFQQRLGESPQSLITTV
                     ITPGLDGGPKQSKSLGNYIALTDSATDKFGKAMSLPDELVGIWAGAYTTLPDEVVAAL
                     GHDAARGGRIA"
BASE COUNT         1219 a         2706 c         2594 g         1161 t
ORIGIN      
        1 gagcgcgtag cggagcagcg ccgctgcggt ccgtgcgggc agcggtgtgg gcaggtagcg
       61 caccggaggc gtctcgagag cggatgcggt ggggcgccgc ccccggtcga cggaccagac
      121 ctccacctcg tgtccctggg atcgcaaagc gcgcgagacc tgcgccacgt gctcctcgac
      181 gccgccgatg tgcggagcgt acgacgaggc gatcattgcg atccgcagtc gccggtcgcc
      241 gtcagcggcc actgtcgtcc gctggcgtcc tgccgaaaag ctcggtcatg acgtcctcgt
      301 ccgtgctctt ccgggcgccg cgacggcgac ccggttcggg cgcggtcacg gcctcggcgg
      361 aggccgcagg agtcgcggcc ggcttgtcct ctgcgcgtcc aggggcggca tccgagttcc
      421 gcggctgctt cgcgtcgtag gcgtaggcgc cgtaggcgcc ggcgttgggc accgccaccc
      481 ggttcagcac gacgccgagc acgtggccgt ggatcttctc cagcacgccg accgcgtcgt
      541 cgacggtgtc gtagctggtg tgcccggcgg acacgacgat gagggcaccg tcagcggccc
      601 gggtgagcac agcaccgtcg gtgacggcga gcagcggagg cgcgtcgatg atcacgatcg
      661 catgcttggc gaggtccgcc agcagcttgt gcatccgctc cgaccccgcc agctcgctgg
      721 ggttcggcgg gagggttcct gccggcagca cgagcaggtt cggcacaccc tgcacgcgac
      781 gcagggcatc cgtgatctcc agccgcccgg cgagcacgtc ggtgaggccg accgcaccgt
      841 cgactccggt gctctccgcc acggtggggc gacgcaggtc gccgtccacg aggatcacgt
      901 gggcgccgtt ggccgccagt gtgccggcga ggttcgccgc caccgtcgac ttgccctcac
      961 ccggcagcgc gctggtgacc acgatgcggc gcggagggtt gtcgacgttc atgaactgca
     1021 ggttggtgcg cagcatccgc agggactcgc gcacggcgaa gtccgcgcgc tgcgccctgg
     1081 cgtcctcaga gacgtcgccg ggacggtaca ggcgatggcc gtcggagagg gaacccacat
     1141 caggcagggt gccggcgacg gggatgtgca gtcgctgctc cacgtcggcc gcggtgcgca
     1201 gccggcgatc ggacatggcg cgcaccagcg cgaacgcgat gcctccaccc agccccagca
     1261 cgatgccgac gatcatggcg agcctggtgt cggggaagac gggagacgac ggcagggatg
     1321 ccgattcgcc gaggttgatc gtcagttcgg cactccccgg attgccgtcg ccctcgacgc
     1381 gatcgatctc cgcggcgagc gcagtgaccc atgccgaagc gatgtcgcga gcggcttccg
     1441 gcgtcgccgc gcgcgcggag atcttgagga tcgccgtgcc ctgcgggttc tccacggtga
     1501 tcagccgagc cagcgcctcg ggcgacagct tcgagccgat ctcctcgccg gcgcgctccg
     1561 ccacggagcg ccaggtggcc atgtcgaggt acgtcggcac cttcgccttg gcgagcccgt
     1621 cgacctgacc gggagagaag atggaagtgt cgaacttgct gcctgcggtg atgtaaccgc
     1681 tggcatcggc ctggtacacc ggggtctgca gcttggccca gccgaagccc gcccccgcgc
     1741 cgaccgcgat catcagcagt atcgccagcc agtagcggcg gaacgcgttg ccatagtcgc
     1801 gaagcctcat ccccgcaccc ctctgtctcg ctcgatcata tgtgctgggt ccttcgtacg
     1861 cccgatctga ccagcacccg ttcgggcagg tcgagatcgc ggtctcgtcg gctcttcttc
     1921 tcccgtatcg cccagaacgc ctgggtggga ccgtgctcat gggcgagggc ctgcgcgccc
     1981 aagcagatgc cagcgacgaa gaacgggacc gacacctggg cggcgaccca gaagaggtcg
     2041 aactgcgcct gggcgatgcg cgcgaggacg aggccgaagg ccagcatccc gaatctcggc
     2101 gccacgcgcc acagcgcgat caggatcacc gcccacatcg cgatgaaccc gatcaggccg
     2161 acgatcccgg tcgaggcgag cacctcgacc tctgcctgcg ggggctggaa gttcgccccc
     2221 ggctgcacgt accagtaccg cagtccgtgc ccgaagacgg gcgaatgctt ccagaacgcg
     2281 tacacctcgc gcagccagtc gacccgctgg tagaaggagt tgaaccggtt ctgcgagtcg
     2341 atctgctcga acacgctctg cacgaccagg tagatgcccg ggatggcgag cacgatgatg
     2401 agcacgatgt gcttggcggc gcctcgtcgc agggtgtgca cgagaagcgc gagagccagt
     2461 ccgatgccgg cctgcctgga ctgcgtcagc agcagggcga tcaggaagac gacgaacgcg
     2521 gcccgggtcc agcggcggtc gatgcgggcc cagtcgggat tcacataggc gatgaaggcg
     2581 gcgaagccga gcatgccgcc ggcgaagttc ttgtgcatcg gccacggcca gttggggtag
     2641 acagcgccga actccccgtg cagggcgtga ccgatcgcgg tgccgaaggt gcccgccgcg
     2701 atcactcccg ctacccccag gatgaggatg aaggccaggc gcgcgcggcc ggcccggccg
     2761 atcacccatc ccacgatcag cgcacccgag accagcagcc aggcgtgcac ccactcgacg
     2821 gtgttctgca cgtacgggtt gacgatcacc gtgagcaggg aggcgaactg gtagccgagg
     2881 ttcgcccaga gcatcacgcg catgggcggg ctgtaggcgt gctttccgag cagcacggcg
     2941 gcgccgagag ccgcggcgag cgccgcgtcg gagacggtga ggtcggcacc gcccagtccg
     3001 aggcgctcgg tgacgagcag ggcgggcgtc gccatcaggg cgatggcgag cggctcggat
     3061 gccgtgagca cgacgcccac ggtgagcgcg gcgacgacgg atgctgcgac agcgccgttc
     3121 gggatgccgc ggtcgagcag caggtagacg gcgaacacga ctccgagcgc gaaaaggcac
     3181 cagcgccagg catccgccca gaactcaccc aaccgcgacc tcacggtccc cgtccccatc
     3241 aggcaagtat cccccacctt tcagttcggg ggtctgagcc tctcgatgcg cctcggcgat
     3301 cgttttccgt cgctcttcga tcagtcagcg acacggccgg gttcactatg cgacatgtcc
     3361 gccattgtcg gaactgtcat ctggcatcgg ccgctccccc gagtggcacg gcgagttgac
     3421 gtctcgccgc tcccccgggc ggcactgctc ttcttcacag cgccttgcgt acgaattcca
     3481 ggagaaacat gaccatcgaa aacaccaacg tctcgcgtcg taccctcgtc aagggcgccg
     3541 cctggtcgct tcccgtcgtc gccgtcgccg ccgccacccc gctggctgct gcttcgacca
     3601 cgaacgcctc gctcgcttgg accggaacga acaccggcct gctgcagctt cagcttctgg
     3661 atggcacggg tgtgctcggc gcacaggtcg ccatcaccgt cccggacgag tacacgatca
     3721 ccaacggcgc gggcgccatc accgccgaga acgccactgt caccatcacg gtgggccgcc
     3781 cgagtggcat cccgatctcg gtcggccgcg cccgcggctt cggcgtcgcg tcggtggatg
     3841 gcatcgccac cacgtcaggt gagcgcacga cgacgtacca gaccgttccc gtcgtgggca
     3901 cggagttcgg cttcccgatc accacctgga gcggtacccg ttccgtgact gtcgcaagca
     3961 acggcactgt cgcagtgccc gtggtcttcg gcctcgccgg ccagtcagac ctgctgtcga
     4021 tcacggcact ggcttcgttc ccggtcaacc tcaccgttgc tttcgccgac ggttcgtcgt
     4081 acacggcatc ctccgcgatc tcggttccgg tcggagctgg cgtcctctga tccgtctcat
     4141 catcgaaagg cgcgtcccgt cccggggcgc gcctttcgct ttcccgcacg acaacaccgg
     4201 ccactccgat aggggatgat ggaggcgttg ctttctgggg aggaaaagta catgcgtctt
     4261 tctgtgttcg gctgcggcta tctcggtgcg gtgcacgcag ccgcaatggc atccatcggc
     4321 catgaggtgg tcggtgtgga cgtcgatccc cgcaagatcg agtcgctcgc caagggcgag
     4381 gcgccgttct tcgagcccgg tctgcagcag ctcctgtcgg agggcatcgc gtcgggccgc
     4441 ctgtcgttca cgacggatgc ttccgccgcc cacggcgcga aggtgcattt cgtcggagtc
     4501 ggcacccctc agcaggccgg gggccatgcc gccgacctaa cctacgtgaa cacggcgatc
     4561 gacgacctgc tcccccatct gtccgcgggc gacatcgtcg ccggcaagtc gacggtgccc
     4621 gtcggcacgg cggccgagct ggcgccgcgc atcgccgccg ccggcgccgt gctcgtgtgg
     4681 aatccggagt tcctgaggga ggggtgggcg gtgcaggaca ccatcgaccc cgaccgcctg
     4741 gtcgccggtg tcccctccgg tgaggagggc cagcgcgcgg cagaggtgct gcgcgaggtc
     4801 tatcacccgg ctgtcgcgaa gggcaccccg ttcatcgtca ccgactacgc caccgcagag
     4861 ctggtgaagg tgtctgcgaa cgcgttcctc gccaccaaga tctcgttcat caacgccatg
     4921 gccgagatcg ccgaggcgac cggggcggat gtcacgcagc tcgccgacgc gataggccac
     4981 gacgcgcgga tcggtcgcaa gttcctcggt gcaggcatcg gattcggcgg cggatgcctg
     5041 ccgaaagaca tccgcgcttt cgccgcgcgg gcggaggagc tcgggcgggg cgagtcggtg
     5101 aacttcctgc gcgaggtcga tgcgatcaat ctgcgccgcc gcgaacgtgc ggtccagctc
     5161 gtcgtcgatg gtctgggcgg cagcgtgtac ggcaagaagg tggctgtgct gggcgcagcg
     5221 ttcaagccgc acagcgatga cgtgcgcgac tcccctgcac tcgatgtggc cgtccgactg
     5281 aacggtctgg gcgcggacgt cgtcgtgacc gatcctcagg cgatcgagaa cgcgcgccga
     5341 ctgcacccgc agctgacgtt cgaagcggat cgcgacgaga cgatccgcgg cgccgcggcc
     5401 gtggtgatgg tgaccgaatg ggacgagtac gctggccgcg accgacccgg cgaacccgta
     5461 cggcgcagcc ctcgcgtggc cgcggctcga tgaggtctcc caccgcccgg gccgcaaggc
     5521 cggcgggctc gtggtgctcg tcgacggggc gctcgtgctc tacctggagc gcggcggccg
     5581 caccgtgctc gcgttcgacg acgatcccga ggtgctgcga gccgcggcgt ccgatctcgt
     5641 cgcgacctcc cgcgcccggc gcctcgagac gctcacggtc gagaagatca acggtgccgg
     5701 cgtctacggc acgcccttcg cgctcgccct gcaggaagcc ggcttcgtcg cgaccccgcg
     5761 cggctatgcc ctccgcaagg cggtgtgaac acgccgcgtc ccgcggacga cggtcaggcg
     5821 gcagccagca ggcgaccttc gcgaatcgcg gcatccagcc cctcttcggc agcccggcgt
     5881 ccctcggcga tcaggcgtgg cacatccttc gtcatccact gagccatgcg caccatgtcg
     5941 ggctggatca ggatgaccgt cggatccgcc accaggtctg ccgtggccgc agtcgtgcgc
     6001 atcatccgca cgttctccca cttcgcatgc agccgcacga tgatggtccg cgtggccccg
     6061 agagcgcgcg tcaccccgtc gggcacgttg tcgatcatgc cgccgtccgc cagcagccgg
     6121 ccgctgcgcc gaacaggagg aagcaggcca ggcacggcga tcgtggcgcg gagggcactg
     6181 ctgagcgggc cgtgatcgag gatcacccct cggcgcgtgc gcaggtcggt ggcgtaggcg
     6241 ccgaacctcc gcggcagatc ctcgatcatc gggtcctcac ccaggatgcg gtgcacggcg
     6301 tcagtcaccg catgggtgtc gagcacgccc caccgcgggc gcgccgacca gcgggcgatg
     6361 tgactccagc ggaatctgcg gacggcgctc tcgatggcgt ctgcatccag ccgagcggca
     6421 tacgcggcgc cgatgagcgc tcccgcgctg gtgcccgcga cgatcccggg tcggatgccg
     6481 cgctcctcca gcacctgaag cacgcccgcc tgggcggcgc cgtaggcacc gccgccgctc
     6541 aacgtcagcc ccagcgtcag gtcgtcaccc atcggagttc ccctcatgaa cgttgcggcg
     6601 acgctatcag catccggctg agcatccggc catcgcccgt gctaacctgc tccgcatgac
     6661 ggcgcgtcag cacacctatc tcccggctct ggtcagagcc ggcgatcagt agcgcccgac
     6721 aaggccagag ccccaggcaa ggacgaacgt ccttgccttt cgtgtatcca cgggaggctc
     6781 tgccccgtca ccctctcgtg cacccatcga gaggaacatc atgacccgcg cagacgacat
     6841 cctgcgtccc atgctccgca ccatcgacgc gatcgtcgac gaacatgcgc tgcgcgccaa
     6901 gctcgagagc ggccggcagc tgcgcatcaa gtacggcgtc gacctcaccg cacccgacct
     6961 gcatctgggt cacgcggtga acctctggct catgcggcac ctgcaggaca acgggcatcg
     7021 cgtggtcttc ctgctgggcg acctcaccac ccgcgtcggc gacccgactg gcaagagcga
     7081 gacccgcccc gtgctcacgg cggagcagat cgagcagaac gcgcaggcct tcctcgacca
     7141 ggtctcgatc gtgctggaca ccgcaccaga ggtgttcgag gtgcgccgga actcggaatg
     7201 gttcgatcgg atgccggtcg ccgagctgct gagcctgttc agcatgacca cgcacgcgca
     7261 gctgatggcc cgcgacatgt tccgcgagcg cctcgcccag gggcgtgaga tcgcgctgca
     7321 cgagctgacc taccccgtgc tgcagggcta cgacagcttc gccatgcaga gcgatctgac
     7381 catcgtcgga tccgatcagc tgttcaacga gatgatgggc cgccagttcc agcagcggct
     7441 cggcgagagc ccgcagtcgc tgatcaccac cgtcatcact ccgggcctgg acggcggacc
     7501 caagcagtcg aagtcgctcg gcaactacat cgcgctcacc gacagcgcca ccgacaagtt
     7561 cggcaaagcg atgagcctgc ccgacgagct ggtcggcatc tgggccggcg catacacgac
     7621 cctgcccgac gaggtcgtgg ccgcactggg ccacgacgcg gcccgcggcg ggcgcatcgc
//
LOCUS       DATMSX010000136         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_182073,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000136 DATMSX010000000
VERSION     DATMSX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182073"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /gene="pepN"
                     /locus_tag="VN041_04130"
     CDS             complement(<1..1908)
                     /gene="pepN"
                     /locus_tag="VN041_04130"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWU28268.1"
                     /translation="MSSANVTANLSRDETAERAAHITVHSIRVELDLSGAPERARTGF
                     PTVTTLNFDATATETWIDFIGESVSRVVVNGEEQDVRWDGARISIEGLKAQNEVRVEA
                     VAEYSRSGEGMHRFHDPVDGETYLYTQYEPADSRRVMACFEQPDMKAAYTFDVSAPAG
                     WHVLSNQSAERVHEGIGVQRIEFAPTLPISSYITAVAAGPYARIDGEWSREGQHIDLG
                     VLCRASLAEHLEADEILEVTRQGLDFFSDAFAYPYPWGKYDQIFVPEYNLGAMENPGL
                     VTFTEAYINRGAATDAQRGGRANTILHEMAHMWFGDLVTMRWWDDLWLKESFADYMGS
                     HASAAATRFTDAWARFANSRKAWAYRQDQLPTTHPIVADIPDLEAAKLNFDGITYAKG
                     AAVLKQLVAFVGEDAFFEGARRYFAAHAFGNTTLDDLLDKLGEVSGQDLRAWSRAWLE
                     TSGVSALRLETDADGRRLLVQDGGAAGPRPHRLRIGLYDLDDGRLALRERIELGIDQE
                     RTEVEIPDADLTLLNDDDLTYAKARLDERSLTAVEESLSTLEDDLVRGLVWSSLWNAT
                     RDGELAAVRYLEIVRRHAPAESNVALLTTVLLNAAFAVRHYVADDARDEQSRAWVDAA
                     WSALQSADAGSD"
     gene            2003..2530
                     /locus_tag="VN041_04135"
     CDS             2003..2530
                     /locus_tag="VN041_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28269.1"
                     /translation="MERDELSTAQLRVLSHPMRLAFLRLLREQGPATSRILGREFELD
                     SGAASYHLRRLEAGGLIAEEPGMGTLRERWWRATADALQFDAAEHDDDAVTRQYVQST
                     VLMNAEELYRVAADVPGADAAWLAESAFIDLQLPLDGAGRRALRAELMEVIDRHRAAE
                     EGPITQIRLQIYGRP"
     gene            2533..>3352
                     /locus_tag="VN041_04140"
     CDS             2533..>3352
                     /locus_tag="VN041_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28270.1"
                     /translation="MGIRGDAARWVVAYTLAILGDVAYYLVLTWAAAEVGGAGWSGLI
                     LAAGSVPRLLLLLVGGLLADRIDPRRIAIWTDTARAVTLIAASALTALLGVLPWWLLA
                     VAVIFGIVDACFIPAVGAMPARLVATDDLPRLQAWRLSGLRIGNAVGPLLGAVLLPAG
                     GTAAFAVLAVLFLASVLLLRGIRPHTASVAGDAPDPLVTGSGWQTIRRLRIAPLVIGT
                     ALSELPFSGPIGVAMVLLAQERDWHPGMAGAMLTAFSLGGLAISLLMSALPRAGG"
BASE COUNT          451 a         1191 c         1161 g          549 t
ORIGIN      
        1 gtcgctgccg gcatccgccg actgcagcgc ggaccacgcc gcgtccaccc acgcgcggct
       61 ctgctcgtca cgcgcatcgt cggcgacgta gtggcgcacg gcgaacgcgg cgttcagcag
      121 cacggtggtc agcagagcca cgttggactc ggcgggcgcg tggcgacgga cgatctcaag
      181 gtagcggacc gccgcgagct caccgtcccg ggtcgcgttc cacagcgacg accagacgag
      241 gccgcgcacc aggtcgtcct cgagcgtcga cagcgactcc tccacggccg tcagcgaccg
      301 ctcatccagg cgcgccttcg catacgtcag gtcgtcgtcg ttgagcaggg tcaggtccgc
      361 atccgggatc tccacctcgg tgcgctcctg atcgatgccg agctcgatgc gctcgcgaag
      421 cgcgaggcgg ccgtcgtcca ggtcatacag gccgatgcgc agacggtgcg ggcgcggtcc
      481 cgcggcgccg ccgtcctgca cgaggagacg ccggccgtca gcatccgtct ccaaccggag
      541 agccgagacg ccactggtct cgagccaggc gcgcgaccag gcgcgcaggt cctgcccgga
      601 gacctcgccg agcttgtcga gcaggtcgtc gagggtggtg ttcccgaacg cgtgcgcggc
      661 gaagtagcgg cgggcgccct cgaagaaggc gtcctcaccc acgaacgcga ccagttgctt
      721 gagcaccgcc gcgcccttgg cgtacgtgat cccgtcgaag ttcagcttgg ccgcctccag
      781 gtcggggatg tcggcgacga tcggatgcgt ggtggggagc tggtcctgcc ggtacgccca
      841 ggccttgcga ctgttcgcga agcgcgccca cgcgtccgtg aagcgtgtcg ccgcggcgga
      901 ggcatgcgac cccatgtagt ccgcgaagga ctccttgagc cacaggtcgt cccaccagcg
      961 catggtgacc aggtcgccga accacatgtg cgccatctcg tgcaggatcg tgttggcgcg
     1021 gccgccgcgc tgcgcgtcgg tcgccgcccc gcggttgatg tacgcctcgg tgaaggtgac
     1081 caggcccggg ttctccatgg cgccgaggtt gtactccggc acgaagatct ggtcgtactt
     1141 gccccaggga taggggtagg cgaaggcgtc gctgaagaag tccaggccct gtcgcgtgac
     1201 ctcgaggatc tcgtcggcct cgaggtgctc ggccagtgac gcgcggcaca gcacgcccag
     1261 gtcgatgtgc tggccctcgc gcgaccactc gccgtcgatg cgcgcgtagg gaccggcggc
     1321 gaccgcggtg atgtagctgg agatcggcag cgtcggcgcg aactcgatgc gctgcacccc
     1381 gatgccctcg tgcacgcgtt cggccgactg gttcgagagc acatgccatc cggcgggggc
     1441 cgacacgtcg aacgtgtagg cggccttcat gtcgggctgc tcgaagcacg ccatcacccg
     1501 gcgcgagtcg gccggctcgt actgggtgta cagataggtc tcgccgtcca ctggatcgtg
     1561 gaaccggtgc atcccctcgc cggagcggct gtactcggcc accgcctcga cgcggacctc
     1621 gttctgcgcc ttcaggcctt cgatcgagat gcgggcgccg tcccagcgga cgtcctgctc
     1681 ctcgccgttc acgaccacgc gggagacgct ctcgccgatg aagtcgatcc acgtctccgt
     1741 cgcggtggca tcgaagttca gcgtggtgac ggtcgggaaa ccggtgcggg cccgctcggg
     1801 tgcgccgctc aggtccagtt cgacgcggat gctgtggacg gtgatgtgcg ccgcccgctc
     1861 cgcggtctcg tcacggctga ggttggcggt cacgttggca gaactcattg ctccatcgtg
     1921 tcacgtcagc ccggtctcgc ctcccccagc cctgcctgag atctagttgc gaagagttct
     1981 tcgcaacatt agcctggcgg acatggaacg cgacgagctc agcaccgcgc agttgcgcgt
     2041 gctctcccac cccatgcgcc tggccttcct ccgtctgctg cgcgagcagg gaccggcgac
     2101 gtcccgcatc ctcggtcggg agttcgagct cgactccggc gccgcgagct atcacctgcg
     2161 ccgcctcgaa gcaggcggtc tcatcgccga ggaacccggc atgggcacgc tgcgggagcg
     2221 ctggtggcgc gcaaccgcgg atgccctgca gttcgacgcc gccgagcacg acgacgatgc
     2281 ggtcaccagg cagtacgtgc agtcgaccgt gctcatgaac gccgaggagc tgtaccgcgt
     2341 cgcagccgac gtgccggggg ccgatgccgc ctggctcgcc gagtcggcct tcatcgacct
     2401 tcagctcccc ctcgacgggg cgggtcgccg cgcccttcgc gccgagctca tggaggtgat
     2461 cgaccgccac cgtgccgctg aggaaggccc gatcacccag atccgtctgc agatctacgg
     2521 ccggccgtga tcatgggcat ccgcggcgat gcggcacgat gggtggtcgc gtacacgctg
     2581 gcgatcctcg gcgacgtcgc gtactacctg gtcctcacct gggctgctgc cgaagtcggt
     2641 ggcgcgggct ggtccggcct gatcctggcg gccggctcgg ttccgcgact gctgctcctg
     2701 ctggtcggag gcctgctcgc cgaccgcatc gatccgcgcc gcatcgcgat ctggacggac
     2761 acggcacgag cggtgaccct catcgccgcg agcgccctca ccgcgctgct cggcgtgctc
     2821 ccctggtggc tgctggccgt cgccgtgatc ttcgggatcg tggatgcatg cttcatcccc
     2881 gcggtcggcg cgatgcccgc ccggctcgtg gcgaccgacg atctcccccg cctccaggcg
     2941 tggcgactca gcggactgcg gatcggcaac gccgtgggtc cgctgctcgg ggctgtgctg
     3001 ctgcctgcgg gcggaactgc cgcgttcgcg gtgctcgccg tcctcttcct cgcctccgtc
     3061 ctgctgctgc gcggcatccg gccgcatacc gcctctgtgg cgggcgacgc accggacccg
     3121 ctggtcacgg gatcgggctg gcagacgatc cggcggctga ggatcgcacc cctggtgatc
     3181 ggaacggcgc tgtccgagct gccgttctcc gggcccatcg gcgtggcgat ggtgctgctc
     3241 gcgcaggagc gcgactggca tcccggcatg gcgggcgcca tgctgaccgc cttcagcctg
     3301 ggcgggctcg cgatcagcct gctgatgtcg gccctgcccc gtgccggcgg cc
//
LOCUS       DATMSX010000137         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_143690,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000137 DATMSX010000000
VERSION     DATMSX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143690"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VN041_04145"
     CDS             complement(<1..315)
                     /locus_tag="VN041_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28271.1"
                     /translation="MTNTLVSVFVPIGVAGAVIALVCALVAAFAIARGAAGLAGGAIG
                     VWIVGALLSVSASFAALWTPFFVAAIGLGVALVAGTVLRVAFAPLLARRAAAVEQAEV
                     ESA"
     gene            complement(466..1950)
                     /locus_tag="VN041_04150"
     CDS             complement(466..1950)
                     /locus_tag="VN041_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWU28272.1"
                     /translation="MATTLITGRPWRVILAFSVPLLIGNVVQQLYQFVDAVVVGRHLG
                     VDSLAAVGATGSLIFLVIGFAWGLASGFAIPTAQAFGASDAHGVRRSVATGTLLTAAT
                     SLVLTVVGPLIAEPFLRLLQTPPELLAEATAFTQVTFIGGSTIMFFNYLAAIIRAIGD
                     STTPLVFLTISCALNVGLVILMVGTLEWGVAGAAWATVVAQAVSVLLCLLYVWRRVPV
                     LHVHRADWKVSRADMAEHLRLGLPMGFQASIIAIGALIVQVALNTLGADAVAAYTAAS
                     RVDSLATAFLASLGLAASMYAAQNLGARRPDRVRRGTIQALWMAVVAALLLGAFMIAF
                     GVPVVRLFVGDGADRVVEYAHLMLIINGSTYVLLGVLFVLRGVLQGLGRVLVPTVTGV
                     IELVARVAAALALGAILEFEGVAMSNPLAWLGAVALLVPAYVSAHRDLAKVPVAPLET
                     TLTTPIPVIGPTDGSMVVDAVFTAPVPVVPPRLTRLRLPRRTRR"
     gene            2146..>2994
                     /locus_tag="VN041_04155"
     CDS             2146..>2994
                     /locus_tag="VN041_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWU28273.1"
                     /translation="MTRLRRSDPHGPGLSRVRRGRGWEYRMPDGTRITDAVLLERLRA
                     MAIPPAWTDVWISPHENGHVLATGMDDAGRRQYIYHPAWHERMAREKFDRMLQLAAVM
                     PGVRAGVTRDLRQDGFGRPRVLAGAFRMLDSAMLRVGSEQYARQHGSIGLATLRGSHA
                     HVLAERTVELAFPGKSGQAWESRVEDPDLAALVAGLKRRGPRALLLSWQDGSTWHPIR
                     PANINDDVRARTGGDFTAKDFRTWAGTVLASLALSEFERFDSQAQARKNIVRAIERVA
                     ERLGNTP"
BASE COUNT          523 a         1015 c         1095 g          361 t
ORIGIN      
        1 ggccgactcg acctcggcct gctcgacggc ggcggcgcgc cgtgcgagca gcggtgcgaa
       61 tgccacgcgg agaaccgtcc cggccacgag tgcgacgccg agcccgatcg cggcgacgaa
      121 gaacggcgtc cacagggcgg cgaaggatgc cgagacgctg agcagcgcgc cgacgatcca
      181 cacgccgatc gccccgcctg cgagcccggc agcgccacga gcgatcgcga aggctgcgac
      241 gagcgcgcag acgagggcga tgacagctcc ggccaccccg atcggcacga acaccgatac
      301 cagcgtgttc gtcaaaccga gtgttgacat tctgacgacc ccccgtcacg gacacgcatt
      361 cgcgtgttcc tgaactcaag gttacgcggg tggattcgcg gagtcactcc ccctgaagaa
      421 cgattcggca tcatcctgaa ggatgacgcg tgaacgcccg gtgactcagc gacgggtgcg
      481 gcgcgggagg cgcaggcggg tcagccgagg cggcaccacg ggcaccggcg cggtgaacac
      541 ggcatccacc accatcgatc cgtcggtggg gccgatcacc gggatcggcg tggtgagcgt
      601 ggtctcgagc ggcgcgaccg gcaccttcgc gagatcgcga tgcgcgctca cgtacgccgg
      661 gaccagcaac gcgaccgcgc cgagccaggc gagcgggttg ctcatcgcga cgccctcgaa
      721 ctccaggatc gcgccgagcg cgagcgcggc cgccacacgg gcgaccagct cgatcacccc
      781 ggtgacggtg ggtacgagca cccgtcccag tccctgcagc accccgcgca gcacgaacag
      841 cacgcccagc agcacgtagg tggagccgtt gatgatcagc atcaggtgcg cgtactcgac
      901 gacgcggtcc gcgccgtcgc cgacgaacag ccgcaccacc ggcacgccga acgcgatcat
      961 gaaggcaccg agcaggagcg cggcgaccac cgccatccac agggcttgga tcgtgcctcg
     1021 gcgcacccgg tcggggcgac gcgcgccgag gttctgcgcg gcgtacatcg acgcggccag
     1081 ccccagcgag gcgaggaatg cggtggcgag gctgtcgacg cgggatgccg cggtgtaggc
     1141 ggccacggca tccgccccca gcgtgttcag cgcgacctgc acgatcagag cgccgatggc
     1201 gatgatcgac gcctggaagc ccatcggcag gcccagacgc aggtgctcgg ccatgtcggc
     1261 gcgcgacacc ttccagtccg cacggtgcac gtgcagcacg ggcacgcggc gccacacgta
     1321 cagcaggcac agcagcaccg agacggcctg cgcgacgacg gtcgcccagg cggctcctgc
     1381 gacaccccac tccagcgtgc cgaccatcag gatcacgagc ccgacgttca gggcgcagga
     1441 gatggtgagg aacaccagcg gcgtggtgga gtctccgatc gcgcggatga tcgcggccag
     1501 gtagttgaag aacatgatgg tgctgccgcc gatgaaggtc acctgcgtga acgcggtggc
     1561 ttcggcgagc agctcgggcg gggtctgcag cagccgcagg aacggctcgg cgatgagcgg
     1621 ccccaccacc gtcagaacca ggctggtggc agcggtgagc agggtgcccg tggccaccga
     1681 gcgccgcacg ccgtgagcat ccgaggcgcc gaacgcctgg gcggtcggga tcgcgaaacc
     1741 gctcgccagt ccccacgcga agccgatgac gaggaagatc aggctgccgg tcgcgccgac
     1801 ggccgcgagc gagtccacgc cgagatggcg gccgacgacc acggcatcca cgaactggta
     1861 cagctgctgc acgacgttgc cgatgagcag cggcacggag aaggccagga tgacgcgcca
     1921 cgggcggccg gtgatgaggg tggtagccat gcgggtccca gggaagcggg cgggcggggt
     1981 ggggatgccg gagacgacga ccgtcaccgt gatcggtcac ggcgacggat cgcagaagtc
     2041 tatcgaatcg tttcgagctt gggaatgcgc gcgacggata tcgtgcgggg gcggtgcggg
     2101 tccaggggct tgtccgcgtg ctcgccgaga cgctagagca ggaggatgac acgactgaga
     2161 cgcagcgatc cgcacggacc cggactgagc cgggtccgcc gcgggcgcgg ctgggagtac
     2221 cggatgcccg acggcacccg catcacggat gccgtgctgc tcgaacggct gcgtgcgatg
     2281 gcgatccctc ccgcgtggac cgacgtctgg atcagtccgc acgagaacgg tcacgtcctc
     2341 gcgaccggga tggacgacgc cggacgccgg cagtacatct atcacccggc ctggcacgag
     2401 cggatggcga gggagaagtt cgatcggatg ctgcagctcg ccgccgtgat gcccggcgtg
     2461 cgcgcagggg tgacgcgcga cctgcgtcag gacggcttcg gtcgtcctcg cgtgctcgcc
     2521 ggggccttcc ggatgctcga cagcgcgatg ctgcgggtgg gctctgagca gtatgcccgg
     2581 cagcacggca gtatcggcct ggccacgctc cgcggatcgc acgcgcacgt cttggcggag
     2641 cgcacggtgg agctcgcgtt ccccggcaag agcgggcagg cgtgggaatc gcgggtcgag
     2701 gaccctgatc tggcggcgct ggtcgccggg ctgaagcgcc gcgggccgcg agcgctgctg
     2761 ctgagctggc aggacgggtc gacctggcat ccgatccgcc cggccaacat caacgacgac
     2821 gtccgcgcgc gcaccggagg ggacttcacc gcgaaggact tccgcacgtg ggccggcacc
     2881 gtcctcgcct cgctcgccct gagcgagttc gagcggttcg actcccaggc gcaggcccgc
     2941 aagaacatcg tccgggcgat cgagcgggtc gccgagcggc tgggcaacac gccg
//
LOCUS       DATMSX010000138         6539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_162761,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000138 DATMSX010000000
VERSION     DATMSX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162761"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VN041_04160"
     CDS             complement(<1..552)
                     /locus_tag="VN041_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWU28274.1"
                     /translation="MSDAVKLTPLGAIVLALLNEGDMHPYEMMRLLRKRRDDRMVRVS
                     NGTFYHTVARLERDGLIAEVGVDRDGNRPERTTYTLQPAALPVLTDWVRRGLGNADRA
                     AEFRVALAEAHNLPRAEVIELLGARHETLREERDQLHQKLEQARTRGVPEQFLIEVDR
                     HVAMLSGELSWSAGLLERLADPGF"
     gene            complement(618..1184)
                     /locus_tag="VN041_04165"
     CDS             complement(618..1184)
                     /locus_tag="VN041_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28275.1"
                     /translation="MDETRTDRLTLRPPTDADVDPIHRIYSDPRVWQHYPSLRHAERS
                     TTERMVARWRAGWETVGLASWIVRLRDTGEVIGNGGCTLIGAGSEPGDAAVWNLGYRI
                     AAEQHGHGYATELSRAAVDAARALRPETPVIAYLVEHNRASAAVAEKVGLELVHRAPD
                     AGNPDPDVLRLVYADRVLTDAQLATALH"
     gene            1236..2099
                     /locus_tag="VN041_04170"
     CDS             1236..2099
                     /locus_tag="VN041_04170"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWU28276.1"
                     /translation="MSEALSIPGWRHIYSGKVRDLYASEDPADTRLLVVASDRVSAFD
                     FVLSPGIPQKGELLTSLSTWWFQQLADVPNHIAEGEIPDAVAGRAMLAMALEMLPIEC
                     VVRGYITGSGWAEYVHSGTVCGIPLPAGLKNGDRLPEPLFTPAYKAPMGEHDENITFE
                     RVVELVGAERTAELRDTSIALYTRAAAIAEEKGLILADTKFEFGTDADGTLRLADEVL
                     TSDSSRYWDAETWRTGATPDERMASFDKQIVRDWLAANWDKQGEPPALPEEIVERTAA
                     RYRELIERLAG"
     gene            2362..3708
                     /locus_tag="VN041_04175"
     CDS             2362..3708
                     /locus_tag="VN041_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HWU28277.1"
                     /translation="MPLWKIHGDGRTVAPDAVVRPEERLNWPATIAIGVQHVIAMFGA
                     TFLVPILTGFPVPTTLLFSGVGTILFLLVTRNKLPSYLGSSFAFIAPVTAATAANATG
                     ASDGIGSALAGIIAVGVLLTIIGVVVHTAGVKWVDRFLPPVLAGTIVALIGFNLAGAA
                     HNNFAQAPVTATFTLAITILFAVVFRGFLGRISIFLGVIAGYIFAGIRGELDFSKVDK
                     AAWVGLPTFNLPDFVSPGTWAVMAMFLPVVLVLIAENVGHVRGVATMVEDPKINENTG
                     KALIADGISTTLAGFFGGSGTTTYGENIGVMASTRVYSTAAYWVAGVTAILLSMSPKF
                     GEVINSVPAGVLGGVTTALYGLIGVIGIKIWVDNKVDFSRPVNQYTAAVALIMAVGGF
                     QITGEDGFVLGGIVIATVTAIVIYHGGNAIARLRRTGADDPKPLQAVGALGGDPES"
     gene            complement(4010..4627)
                     /locus_tag="VN041_04180"
     CDS             complement(4010..4627)
                     /locus_tag="VN041_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28278.1"
                     /translation="MARSEEQNRLARERTREGILRSAIELFAEQGVHETTIAEITRSA
                     GVAQGLVNYHFGGKDQLVGAVADRWYETLIGLAHGQGAPGEMDADARLRGVIDAALIA
                     AVAHRSLQRVMLSLAHQPSTRRIVAESEQRHSDAVQFAEQAVRSIFAERGATDPALEE
                     GMLRTLLEGIAGRYAASDGSFPIDAARRWVHLQYGLGEPVGGLSA"
     gene            4749..5621
                     /locus_tag="VN041_04185"
     CDS             4749..5621
                     /locus_tag="VN041_04185"
                     /inference="COORDINATES: protein motif:HMM:NF018584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HWU28279.1"
                     /translation="MHPSARVNLNDLGSILEWLIGQWWVWAIVGVVVLFFVVVWLLPD
                     AKVVPSADFSTTDTEANEIALGYMQISNLPSGPWNDPTAPGIGDREKKQLADQWGLHS
                     RQDWLDNIERLTTVRRRRDPWVISLQLRADLAAQLGRVPKTKEWTTAIVEAGGNKRDA
                     RTFVAAIEHIESQVRKLAGKDIVPADAFVKTLDGYALGQAVALATWGVALGYGGVAEA
                     RQLIHRINVEGRPAFDSWVDFGLSYIAGRVMHWSDGNIGDKTFDKLGDNWSDLKAACT
                     EKRNGPWATLSWKL"
     gene            5609..>6539
                     /locus_tag="VN041_04190"
     CDS             5609..>6539
                     /locus_tag="VN041_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28280.1"
                     /translation="MEALNQPLQVELGPLRDRMGIGDLLRYERLAGIPLRRRFFVALG
                     GVLLLAVAMLVALVWLDGTGILARVPLLGFFAVGYAVLAAVGFVWYAIDLTRRARLAL
                     FAWVNGWAYADSLEGTRRPGSAFARTLRSRERGVVASDDERLPFELGRNFSIGTRDSG
                     ATVQRPFAFVELPLPVKVPHIVLKNRRRSIIPTFGLGRGGARMELEGEFAKHFLLLVP
                     EGYQQDALYIFTPDLMARVIDLGAGAEIELVEDRLYVYLPGGTRFDRPATMEATLRLA
                     EEFQRRFAARTELYRDDQAGDLAARAGVAIGLRG"
BASE COUNT          970 a         2366 c         2181 g         1022 t
ORIGIN      
        1 gaacccgggg tcggcgaggc gctcgagcag cccggcgctc cagctgagct cgccgctcag
       61 catcgcgacg tgccgatcca cctcgatgag gaactgctcg gggacgccgc gggtgcgggc
      121 ctgctcgagc ttctggtgca gctgatcgcg ctcctcccgg agggtctcat gccgcgcacc
      181 gaggagttcg atgacctcgg cccgcggcag gttgtgggcc tcggcgagcg cgacgcggaa
      241 ctccgcggca cggtcggcgt tccccagtcc ccggcgcacc cagtcggtga gcaccgggag
      301 cgctgcgggc tgcagcgtgt aagtggtgcg ttccggccgg ttgccgtcgc ggtcgacgcc
      361 cacctcggcg atgagcccgt cccgttcgag ccgcgcgacc gtgtgataga aggtgccgtt
      421 gctgacgcgc accatccgat cgtcccgccg cttgcgcagc agtcgcatca tctcgtacgg
      481 atgcatgtcg ccctcgttga gcagcgcgag cacgatcgcg cccagcggcg tcagcttcac
      541 ggcatccgac atctatccca ccccgattag tccatatgga ctataccact ccccctcgcc
      601 catccgcggg ggacgattca gtgcagagcg gtcgcgagct gcgcatccgt caacactcga
      661 tccgcataga ccagacgcag gacgtcaggg tccggattcc ccgcatccgg cgcacggtgc
      721 acgagctcca gtccgacctt ctcggcgacg gccgccgatg cgcggttgtg ctcgacgagg
      781 tacgcgatga ccggcgtctc cggccgcagg gcgcgtgccg catccactgc cgcgcgtgac
      841 agctcggtcg cataaccatg accgtgctgc tccgcggcga tccggtagcc gaggttccag
      901 accgcggcgt caccgggctc cgatcccgcg ccgatcagcg tgcatccgcc gttgccgatg
      961 acctcgccgg tgtcgcgcag gcgcacgatc catgacgcga ggcccacggt ctcccagccc
     1021 gcccgccacc gcgcgaccat gcgctcggtc gtcgaccgct ccgcatgacg caggctcggg
     1081 tagtgctgcc agacccgcgg atcggagtag atccggtgga tcgggtcgac atcggcatcc
     1141 gtcggcggtc gcagggtcag tcggtcggtg cgcgtctcgt ccatcggctc gacagtacgc
     1201 gcggtctccg acatcgcgga tgccacaatg gacgggtgag cgaagcactg agcatccccg
     1261 gctggcggca catctactcc ggcaaggtcc gcgacctgta cgcctcggag gatccggcag
     1321 acacgcggtt gctggtcgtg gcctccgacc gggtgagcgc cttcgacttc gtgctctccc
     1381 ccggcatccc gcagaagggc gaactgctca cctccctcag cacctggtgg ttccagcagc
     1441 tggcggacgt cccgaaccac atcgccgagg gcgagatccc ggatgccgtg gccgggcgcg
     1501 ccatgctggc gatggcactc gaaatgctgc cgatcgagtg cgtcgtgcgc gggtacatca
     1561 cagggtccgg ctgggccgag tacgtgcaca gcggcaccgt ctgcggcatc ccgctgcccg
     1621 ccggcctgaa gaacggcgac cggctgcccg agccgctgtt cacccctgcc tacaaggcgc
     1681 cgatgggcga gcacgacgag aacatcacct tcgagagggt cgtcgagctc gtgggtgcgg
     1741 agcgcaccgc cgagctgcgc gacacctcca tcgcgctcta cacgcgcgcc gccgcgatcg
     1801 ccgaggagaa gggcctgatc ctcgccgaca ccaagttcga gttcggaacg gatgccgacg
     1861 gcaccctgcg cctcgccgat gaggtgctca ccagcgactc gtcccgttac tgggacgccg
     1921 agacctggcg caccggcgcg acgccggacg agcggatggc cagcttcgac aagcagatcg
     1981 tgcgcgactg gctggccgcg aactgggaca agcagggcga gccgcccgca ctgcccgagg
     2041 agatcgtcga gcgcacagcc gcccgctacc gcgagctcat cgagcgcctg gcaggctgac
     2101 gcgcgccggc agggcatccg cctcgccctg ctgaggcacc ccgccccgct ccggtcccgt
     2161 cccactgcgg tcccgtgtcc cacaaatggt gggtccctcg gcgcgcaacc cgccgtctct
     2221 gcgacaagcg ccgacagcat gtcccagaaa cggcgactcc ggatgctgcg cacccgcacc
     2281 atctgagaca cgccgcgggg caggcgatag ggtttcctcg gccctgatcc ggacccgccg
     2341 ccctactcct gaggagcacc catgccgctg tggaagatcc acggtgacgg tcgcaccgtc
     2401 gcgcccgacg ccgtcgtccg ccccgaagag cgcctgaact ggcccgccac catcgcgatc
     2461 ggcgtgcagc acgtcatcgc catgttcggc gccacgttcc tggtgccgat cctcaccgga
     2521 ttcccggtgc cgaccacact gctcttcagc ggcgtgggca cgatcctgtt cctgctggtc
     2581 acccgcaaca aactgcccag ctacctcggc tcgtcgttcg cgttcatcgc tcccgtcacc
     2641 gctgcgaccg ctgccaacgc caccggcgcc agtgacggca tcggttcggc cctggcgggg
     2701 atcatcgccg tcggcgtgct gctgaccatc atcggcgtgg tggtgcacac cgccggcgtg
     2761 aagtgggtcg accgcttcct gccgccggtg ctcgccggca ccatcgtcgc gctgatcggg
     2821 ttcaacctgg ccggagcagc gcacaacaac ttcgcccagg cacccgtcac cgcgacgttc
     2881 acgctcgcga tcacgatcct gttcgccgtg gtgttccgcg gcttcctggg tcgcatctcg
     2941 atcttcctcg gcgtgatcgc cgggtacatc ttcgccggca tccggggtga gctggacttc
     3001 agcaaggtcg acaaggccgc ctgggtgggg ctcccgacct tcaacctgcc cgacttcgtc
     3061 tcccccggca cctgggccgt gatggcgatg ttcctgcccg tcgtgctcgt gctcatcgcc
     3121 gagaacgtcg gtcatgtgcg cggcgtcgcg accatggtcg aggacccgaa gatcaatgag
     3181 aacaccggca aggcgctgat cgccgacggc atctcgacca cgctcgcggg cttcttcggc
     3241 ggctcgggaa ccaccaccta cggcgagaac atcggcgtca tggcgtccac gcgcgtctac
     3301 tccaccgctg cgtactgggt ggcgggcgtc acggcgatcc tgctgagcat gtcaccgaag
     3361 ttcggggagg tcatcaactc ggtgcccgcc ggtgtgctcg gcggtgtgac caccgcgctg
     3421 tacggcctga tcggcgtgat cggcatcaag atctgggtgg acaacaaggt cgacttctcg
     3481 cgccccgtca accagtacac ggctgccgtt gccctgatca tggccgtcgg agggttccag
     3541 atcacgggcg aggacggctt cgtgctgggt ggcatcgtga tcgccacggt cacggccatc
     3601 gtcatctacc acggcggcaa cgccatcgca cgactgcgcc ggaccggtgc agacgacccg
     3661 aagccgctcc aggccgtggg cgcgctcggc ggcgacccgg agtcctgagt ccgacttgag
     3721 gggcctcgga tgctgcgcac agcggcatcc ggggcccttc ggcgtctccg gcatcggcgc
     3781 gcacgcctcg gtcgggagcg cacccgcgac tcgtgtccca gcacccgcgg gtgcgagcac
     3841 gacagaccca ccgcttctgt gacatgtgcg cctccatgtc ccagaaggcg cggatcgcgg
     3901 catccggaag ccgccactgg tggaacacgc gtgcaggcat gtcccgcagg atgctttctc
     3961 cgccgcccag cgagccgcaa ctacgatcac gccgcccagg gagccgcaac tacgcggaca
     4021 ggccgcccac cggctcgccg aggccgtact gcagatgcac ccagcgtcgc gccgcgtcga
     4081 tcgggaatga tccgtcggag gccgcatagc gcccggcgat gccctccagg agggtgcgca
     4141 gcatcccctc ctccagcgcg ggatccgtcg ctcctcgttc ggcgaagatc gagcggacgg
     4201 cctgctcggc gaactgcacg gcgtcgctgt gccgctgctc cgactcggcg acgatgcgcc
     4261 gcgtcgaggg ctggtgcgcg agcgagagca tgacgcgctg cagcgaccgg tgcgcgacgg
     4321 cggcgatgag cgcggcgtcg atcacgccgc gaagccgtgc gtccgcgtcc atctcaccgg
     4381 gtgcaccctg cccgtgagcg agcccgatca gggtctcgta ccagcggtcg gccacagctc
     4441 cgacgagctg atccttgccg ccgaagtggt agttgaccag gccctgggca acacccgcac
     4501 tacgagtgat ctcggcgatc gtcgtctcgt gcacgccctg ctcggcgaac agctcgatcg
     4561 cggaccgcag gatgccctcg cgcgtgcgct cgcgggcgag gcgattctgc tcctcggacc
     4621 gggccacgca gctcacctcc cgcgacccga cgcgcaggac ttccgcgatc ggggtcgtgc
     4681 cctctacagt actcattgaa cggccgttca atctatggat cggccacctg cattcgaccg
     4741 gagaacccat gcatccttct gcgcgcgtca acctcaacga cctcggctcg atcctcgagt
     4801 ggctcatcgg ccagtggtgg gtgtgggcca tcgtcggcgt ggtcgtgctc ttcttcgtgg
     4861 tggtgtggct gctgcccgac gcgaaggtcg tcccctccgc ggacttctcg accaccgaca
     4921 ccgaggcgaa cgagatcgcg ctgggctaca tgcagatcag caaccttccc tcgggaccct
     4981 ggaacgatcc caccgccccc ggcatcggcg accgcgagaa gaagcagctc gccgatcaat
     5041 ggggcctgca ctcccgccag gactggctcg acaacatcga gcggctcacg acggtgcgcc
     5101 gccggcgcga cccgtgggtg atctcgcttc agctgcgcgc cgacctcgcc gcgcagctcg
     5161 gccgcgtgcc gaagacgaag gagtggacca ccgccatcgt cgaggcgggc ggcaacaagc
     5221 gcgatgcgcg caccttcgtg gccgccatcg agcacatcga gagccaggtg cgcaagctcg
     5281 cgggcaagga catcgtcccc gccgatgcat tcgtgaagac cctcgacggc tacgcgctcg
     5341 gccaggccgt cgcgctggcg acctggggtg tcgcgctcgg gtacggcggc gtcgccgagg
     5401 cgcgtcagct catccaccgc atcaacgtcg agggccgccc cgcgttcgac tcctgggttg
     5461 acttcggcct cagctacatc gccggccgcg tgatgcactg gagcgacggc aacatcggcg
     5521 acaagacctt cgacaagctc ggcgacaact ggtccgacct caaggcggcc tgcaccgaga
     5581 agcggaacgg cccctgggcc acgctctcgt ggaagctctg aaccagccgc tgcaggtcga
     5641 gctcggcccg ctgcgggacc ggatggggat cggcgatctg ctgcggtatg agcggctcgc
     5701 cggcatcccg ctccgtcgtc gcttcttcgt cgcgctgggc ggggtgctcc tgctggcggt
     5761 cgccatgctc gtggcgctcg tgtggttgga cggcaccggc atcctcgccc gcgtcccgct
     5821 gctgggcttc ttcgccgtcg gctacgcggt gctcgccgcg gtcgggttcg tctggtacgc
     5881 gatcgatctg acccgtcgcg cgcggctggc gctgttcgcg tgggtgaacg gctgggccta
     5941 cgcggactcg ctggagggca cccggcgccc cgggtcggcc ttcgcccgca cgctgcgcag
     6001 cagggaacgc ggcgtggtcg ccagcgacga cgagcgcctg ccgttcgagc tgggacgcaa
     6061 cttctcgatc ggcacgcggg attccggcgc caccgtgcag cggccgttcg cgttcgtcga
     6121 gctgccgctg ccggtgaagg tgccgcacat cgtgctgaag aaccggcgcc gcagcatcat
     6181 cccgacgttc gggctcggcc gcggcggggc gcggatggag ctggagggcg agttcgccaa
     6241 gcacttcctg ctgctggtgc cggaggggta ccagcaggac gcgctgtaca tcttcacgcc
     6301 cgacctgatg gcccgcgtga tcgacctggg cgcgggcgcg gagatcgaac tcgtcgagga
     6361 ccgcctgtac gtctacctgc cgggcggcac gcgcttcgac cggcccgcga ccatggaggc
     6421 gacactgcgg ctcgcggagg agttccagcg acgcttcgcc gcgcggacgg agctgtaccg
     6481 cgacgatcag gcgggcgatc tcgccgcgcg ggcgggcgtg gcgatcggcc tgcgcggtc
//
LOCUS       DATMSX010000139         7971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_68317,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000139 DATMSX010000000
VERSION     DATMSX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68317"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VN041_04195"
     CDS             complement(<1..369)
                     /locus_tag="VN041_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWU28281.1"
                     /translation="MAEEDPRIRARLVMEERRRDDVYPADALAAIARAARFDQPPITA
                     EQLSREPDALRDVDVLLTGWGAPVIDAELLARAPRLKAVLHAAGSVKHIVTDASWDRG
                     IEVVSAAAANAEPVAELTAAQ"
     gene            514..1839
                     /locus_tag="VN041_04200"
     CDS             514..1839
                     /locus_tag="VN041_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU28282.1"
                     /translation="MKAAALIGIATLALAGCSSAAPGGAGGTGVKQDEKATLEIWTRT
                     TPGSPSEAATKRQVEAFEKATGYKAEVTAIFDDFEIKLAQRAGQKDLPDIVLNDVSQL
                     GTMQSQGILREIDLKDIKNSKNLTPQALKAGKAVDGKTYGIPYSAQANALLIRSDWLK
                     NLGLEAPKTWDEMEQVAKAFTENDPDGNGKNDTYGLAVPGSTKRGYASWYFSNFLWAA
                     GGDFITGSGDKWLPAMTSDAAVKATTWFRDLQCKAGVVQPGAPSMDTPPTNEAFESGI
                     AGMYVTGPYMMPRFDKVLGADKYEVVPAPKGPKDSAVLAEGGTVYLMAGSENVKGQTA
                     FADWYVSPEAQKFGMEGTDAFAIQLPVNEKVKVSDVRDDARWTVFQDAYQKSGHYAPT
                     IPSWTPVRQMSADTINALLADCSLDVKDELTKLDGKLTAELLTQGILKQ"
     gene            1836..2807
                     /locus_tag="VN041_04205"
     CDS             1836..2807
                     /locus_tag="VN041_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28283.1"
                     /translation="MTIDTADAATAADAPQQAAGAHTSPPPARRRRRHRVQGQWWVPW
                     AFLAPALLLFLIFRYIPMGQAIGMSVENVRPYLGNEYVGMQNYQTILASPSFRDAAVN
                     TLILAVGQTAGCLLLGFLLALLLEGQTRKLSFIRSAAFLPVVVPMAVVAELWRIMYHP
                     SADGFLNSIIGLVGLGPSEFINSPDSSMWSIMVMGIWRGGPYDMMIILAGLAGIDRGL
                     YEAATIDGAGRWQRIRHVTLPGLRPVFTILFILAAIRGFRVFTEVYLMTNGGPNGSTE
                     VIMTLLYKLGLEQQKLGVGSAGAVLLFLATLVLTLVVQLLLRKKKDA"
     gene            2804..3670
                     /locus_tag="VN041_04210"
     CDS             2804..3670
                     /locus_tag="VN041_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28284.1"
                     /translation="MSVGTDTALGLTESRTVVGIGVKVVLYALVVVLFAGPLLALLSG
                     AFSEVSDPTELSVIPNRPTLENFSVAVDFGVLGYLMNSFFVVGFGLLMQVVVSVLAGY
                     ALARKRFRFSALALVAILATMMLPDEILALPLSILLADLPLVHINLMGTLAGMIVPIG
                     AWAFSILVMTEFMKDVPLELEEAARIDGASELRVFAQIIVPLCKPALGVIGVFGFTMI
                     WDQYLLPLLVSTSSATYTLPLALRTLRIEPEVTPGVVMAASLLALAPSVIVFLLFQRS
                     FARGLTSGALKG"
     gene            3674..4417
                     /locus_tag="VN041_04215"
     CDS             3674..4417
                     /locus_tag="VN041_04215"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWU28285.1"
                     /translation="MVVLQGVVGFRDVGGIPAEGGMIRTGRLFRSGHLAAADADGAMF
                     LKTSVARIVDLRSDEEAQADATAVADIPITRLPLYLGSTRSFFEQDFSLGEIYAHLLA
                     ESSAQLVDAVRVIAAGEPTLVHCTAGKDRTGVVIALALSAVGAERDAVVADYARTADL
                     IPAGHRRATSERLTAKYPQSQHAWMLATESPAEVMAQTLSDLDARWGSASAYLRAHGL
                     TDDELAALGAALVEPIPASAPVTTRGAHA"
     gene            4414..5700
                     /locus_tag="VN041_04220"
     CDS             4414..5700
                     /locus_tag="VN041_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWU28286.1"
                     /translation="MTASAAEMAWFTEARLGMFVHWGLYSLAARHEWVKNRERMTDAE
                     YEPYREHFDPDRYDPRAWARTAKAAGAGYVVLTAKHHDGFCLWDSKLTDYTVMNTPYG
                     RDAVAEFVEACRAEGLRVGIYYSLIDWHHEAFPVDGTHPQRDDEEFKAATADRDVRDY
                     QPYLHGQVHELLTEYGRIDYLFFDFSYTHRVDYWGGKGAEDWDAEGLMALIRELQPHV
                     IVNDRMGIPGDIVTPEQYQPLEPMRRDGVEVPWEACQTLNGSWGYDRDNLDVKSPESL
                     IRLLIDGVSKNGNLLLNVGPDGRGRIDRTAQRTFEEIAAWMDDHARSIHGAGPAPWQA
                     PTGTRYTLRGDRLYVHLFDWPFKHLHLPGLAGKVRYAQLMSDASELVPVHIDPNRPAQ
                     NTLMGGLPEGTYTLQLPVQRPDTPVPVVELFLTDPV"
     gene            5775..7256
                     /locus_tag="VN041_04225"
     CDS             5775..7256
                     /locus_tag="VN041_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HWU28287.1"
                     /translation="MKRRTFILSAMAGAATAVVGAQAAGASMFGVGPAASGVVPMLPG
                     VGPYSRASSLAELRAAIAAAPEGGTIIVANGTYVVPEGEPIDLTGVGGRWNKPLMITA
                     ESIGGVTFTGAESFRFGGTHDLVLRGFVFTQSTTFELPADCQSITLSRNDFQLADIEG
                     LLCVMVRADDSVVEYNHFHNKSTLGVFLGVEGAGSTEMAQNVHIHHNYFSDHTFAGDN
                     GGEPIRLGVSPRALSSAHAIVEYNLFERCNGDPEAISVKSSDNIIRYNTIRDSGGGIV
                     LRHGNRNRVESNHIIRGLRGIRIYGNDHVIVNNYIGETADTAFVLGAGTERDHYEGEP
                     AASRRKNDAADRVHIALNTVYGNAGGISCETHRPVTPMDCVIVDNIFQGDAGTLSEVA
                     NSIGFTWAGNLFWGAAPDGDMPASGFTRVDPKLAKDADDVWRIGSGSAAVDAATRDHR
                     AVVRDDIDGRQRLGTADVGAHELSMAAPRRSPLAASEVGPLSR"
     gene            complement(7634..>7971)
                     /locus_tag="VN041_04230"
     CDS             complement(7634..>7971)
                     /locus_tag="VN041_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28288.1"
                     /translation="VAGGRSAARAARDSGATAVVAYNDLIAIGLMQELVETGIRIPAD
                     LSVIGFDDIFGADFTSPSLTTVKSPLREAGDHAMTALLSRVAGDAAPYVGFDLETTLV
                     VRGSTGPAA"
BASE COUNT         1253 a         2813 c         2684 g         1221 t
ORIGIN      
        1 ctgcgcggcc gtgagctcgg cgaccggctc ggcgttcgcc gcggcggccg agacgacctc
       61 gatgccgcga tcccaggacg cgtcggtgac gatgtgcttg accgagcccg cggcgtgcag
      121 cacagccttc aggcgcgggg cacgggcgag cagctcggca tcgatgaccg gcgcgcccca
      181 tccggtcagc agcacgtcca cgtccctcag agcgtccggt tcgcgtgaaa gctgctcggc
      241 cgtgatcggg ggctgatcga accgtgcggc gcgggcgatc gcggccagcg catccgccgg
      301 atagacgtcg tcgcgacgcc gttcctccat gacgagacgt gctcggatgc gaggatcttc
      361 ttcggccacg atgcctccag gaatcgaatt gtggggagcc agacatcgga tcaattgttg
      421 acagtgtccg cgaccctagg ttagattcgc gaacagcgtc ttgcaaagtg gcaagcgttt
      481 gccaacccgg agagagtccc catgcacaca aggatgaaag cagccgcact gatcggcatc
      541 gccacgcttg ccctcgcggg ctgctccagc gccgcacccg gcggcgcggg gggaacgggc
      601 gtcaagcagg atgagaaggc caccctcgag atctggaccc gcaccacgcc gggcagcccg
      661 agcgaggccg ccaccaagcg tcaggtcgag gccttcgaga aggccaccgg ctacaaggcc
      721 gaggtcaccg cgatcttcga cgacttcgag atcaagctcg cccagcgcgc aggccagaag
      781 gatctgcccg acatcgtgct caacgatgtg agccagctcg gcacgatgca gtcgcagggc
      841 atcctccgcg agatcgacct caaggacatc aagaactcca agaacctgac cccgcaggcc
      901 ctcaaggcgg gcaaggccgt ggacggcaag acctacggca tcccctactc cgcacaggcc
      961 aacgccctgc tcatccgcag cgactggctc aagaacctcg gtctcgaggc gccgaagacg
     1021 tgggacgaga tggagcaggt cgccaaggcc ttcaccgaga acgatcccga cggcaacggc
     1081 aagaacgaca cctacggcct ggccgtgccc ggttcgacga agcgcgggta cgcgtcgtgg
     1141 tacttctcga acttcctgtg ggccgcgggc ggcgacttca tcaccggctc cggcgacaag
     1201 tggctgcccg cgatgacctc ggacgccgcg gtcaaggcca ccacgtggtt ccgcgacctg
     1261 cagtgcaagg ccggcgtcgt gcagcccggt gccccgagca tggacacccc gccgaccaac
     1321 gaggcgttcg agtccggcat cgccggcatg tacgtcacgg gcccgtacat gatgccgcgc
     1381 ttcgacaagg tgctcggcgc cgacaagtac gaggtcgtcc cggcgcccaa gggcccgaag
     1441 gactccgcgg tgctcgcgga gggtggcacg gtgtacctga tggcgggctc cgagaacgtg
     1501 aagggccaga ccgccttcgc cgactggtac gtcagccccg aggcgcagaa gttcggcatg
     1561 gagggcacgg atgccttcgc catccagctc ccggtgaacg agaaggtgaa ggtctcggac
     1621 gtccgtgacg acgcacgctg gaccgtcttc caggacgctt atcagaagag cggccactac
     1681 gcaccgacca tccccagctg gaccccggtg cgccagatga gcgccgacac catcaacgcc
     1741 cttctcgccg actgctcgct cgacgtgaag gacgagctca ccaagctcga cggcaagctg
     1801 accgccgagc tcctgacgca ggggatcctc aagcagtgac catcgacaca gccgacgccg
     1861 ccaccgcggc cgacgctccc cagcaggcgg cgggggccca cacgtccccg ccgccggccc
     1921 ggcggcgtcg ccgccaccga gtccagggcc agtggtgggt cccgtgggcg ttcctcgccc
     1981 ccgccctcct gctgttcctg atcttccgct acatccccat gggtcaggcc atcgggatgt
     2041 cggtggagaa cgtgcgcccg tacctcggca acgagtacgt gggcatgcag aactaccaga
     2101 ccatcctcgc ctcgcccagc ttccgcgacg cagcggtcaa caccctgatc ctcgcggtcg
     2161 gccagactgc gggatgcctg ctgctgggct tcctgctcgc actgctgctc gaggggcaga
     2221 cccgcaagct gtcgttcatc cgctccgcgg ccttcctgcc cgtggtcgtc ccgatggccg
     2281 tggtcgccga gctgtggcgg atcatgtacc acccgtccgc ggacggcttc ctgaactcca
     2341 tcatcgggct cgtcggcctg ggccccagtg agttcatcaa cagccccgac tcctcgatgt
     2401 ggtcgatcat ggtgatgggc atctggcgcg gcggccccta cgacatgatg atcatcctcg
     2461 ccgggctcgc cggcatcgac cgtggcctct acgaggcggc gacgatcgac ggcgcgggcc
     2521 gctggcagcg catccggcac gtgaccctcc ccgggctgcg ccccgtgttc acgatcctgt
     2581 tcatcctcgc cgcgatccgc gggttccggg tgttcaccga ggtctacctg atgaccaacg
     2641 gcgggccgaa cggctcgacc gaggtcatca tgaccctgct ctacaagctg gggctcgagc
     2701 agcagaagct gggcgtcggc tcggccggtg ccgtgctgct gttcctggcg accctcgtgc
     2761 tgaccctcgt ggtgcagctc ctgctcagaa agaagaagga cgcatgagcg tcggaacaga
     2821 caccgccctc ggcctcaccg agagccgcac cgtcgtcggc atcggcgtga aggtcgtgct
     2881 gtacgccctc gtggtcgtgc tgttcgcagg cccgctgctc gccctgctct cgggcgcgtt
     2941 cagcgaggtg tccgacccca ccgagctcag cgtcatcccg aaccgtccga cgctggagaa
     3001 cttctcggtc gccgtcgact tcggcgtgct cggctacctg atgaactcgt tcttcgtggt
     3061 gggcttcggt ctgctcatgc aggtggtcgt ctcggtgctg gccggctatg cgctggctcg
     3121 caagaggttc cggttctcgg cgctggcgct cgtggcgatc ctcgcgacga tgatgcttcc
     3181 cgacgagatc ctggcgctgc cgctgtcgat cctgctggcc gatctgcccc tcgtgcacat
     3241 caacctgatg ggcacgctcg ccggcatgat cgtgccgatc ggagcctggg cgttctcgat
     3301 cctggtgatg accgagttca tgaaggacgt ccccctcgag ctcgaggagg cggcccgcat
     3361 cgacggcgcc agcgagctgc gggtgttcgc gcagatcatc gtgccgctct gcaagccggc
     3421 gctcggcgtg atcggtgtgt tcggcttcac catgatctgg gaccagtacc tgctgccgct
     3481 gctcgtgtcg acctccagcg ccacctacac gctgcccctc gcgttgcgca cactgcgcat
     3541 cgagcccgag gtcacccccg gcgtcgtgat ggcggcgtcg ctgctggcgc tcgccccttc
     3601 ggtgatcgtg ttcctgctgt tccagcgctc gttcgcccgc ggtctcacct cgggcgcgct
     3661 gaagggctga cgcgtggtcg tgctgcaggg tgtcgtcggc ttccgcgacg tgggcgggat
     3721 cccggccgag ggcgggatga tccgcaccgg gcgcctgttc cggtcgggtc atctcgcggc
     3781 cgcggatgcc gacggggcga tgttcctgaa gacctccgtc gcgcggatcg tcgacctgcg
     3841 gtcggacgag gaggcgcagg cggatgccac ggccgtggcc gacatcccga tcacccgcct
     3901 cccgctgtac ctcggctcca cccgctcgtt cttcgagcag gacttcagcc tgggggagat
     3961 ctacgctcac ctgctggccg agtcgtcggc gcagctggtg gatgccgtgc gcgtgatcgc
     4021 ggcgggcgag ccgacgctcg tccactgcac ggcgggcaag gaccgcacgg gcgtcgtgat
     4081 cgcgctggcg ctgtcggcgg tcggtgcgga gcgggatgcc gtggtcgccg actacgcgcg
     4141 cacggccgac ctcatccccg ccggccaccg ccgtgccacg tcggagcgcc tgacggcgaa
     4201 gtatccgcag tcgcagcatg cctggatgct ggccaccgag tcccccgccg aggtgatggc
     4261 gcagacgctg tccgacctcg acgcgcgctg gggatcggca tccgcctacc tgcgcgcgca
     4321 cggcctgacc gacgacgagc tggcggcgct cggcgccgcg ctcgtcgagc ccatcccggc
     4381 atccgccccc gtcaccaccc gaggagctca cgcatgacgg catccgccgc agagatggcc
     4441 tggttcaccg aggcgcgcct cggcatgttc gtgcactggg ggctgtactc gctcgccgcg
     4501 cggcacgagt gggtgaagaa ccgcgagcgg atgacggatg ccgagtacga gccgtaccgc
     4561 gagcacttcg accccgaccg atacgacccg cgcgcctggg cgcgcaccgc gaaggcggcc
     4621 ggcgccggct acgtcgtgct caccgcgaag caccatgacg ggttctgcct gtgggactcg
     4681 aagctcaccg actacaccgt gatgaacacg ccctacgggc gcgacgccgt ggcggagttc
     4741 gtcgaggcct gccgcgccga ggggctgcgc gtgggcatct actactcgct gatcgactgg
     4801 caccacgagg ctttcccggt cgacggcacg cacccgcagc gcgacgacga ggagttcaag
     4861 gcggccaccg ccgatcgcga cgtgcgcgac taccagccgt acctgcacgg ccaggtgcac
     4921 gagctgctca cggagtacgg gcgcatcgac tacctgttct tcgacttctc gtacacgcac
     4981 cgcgtcgact actggggcgg caagggtgcg gaggactggg atgccgaggg gctgatggcc
     5041 ctcatccgcg agctgcagcc gcacgtgatc gtgaacgacc ggatgggcat cccgggcgat
     5101 atcgtcacgc ccgagcaata ccagccgctc gagccgatgc gccgcgacgg cgtggaggtg
     5161 ccctgggagg catgccagac gctcaacggc agctggggct acgaccgcga caacctcgat
     5221 gtgaagagcc cggagtcgct catccggctg ctcatcgacg gcgtctcgaa gaacggcaac
     5281 ctgctgctga acgtcgggcc ggacggccgc gggcgcatcg accgcaccgc gcagcgcacc
     5341 ttcgaggaga tcgcagcctg gatggacgac cacgcgcggt cgatccacgg cgccggtccc
     5401 gccccgtggc aggcgcccac cggcacgcgt tacacgctgc gcggcgaccg cctgtacgtg
     5461 cacctgttcg actggccgtt caagcacctg cacctgccgg ggctggccgg aaaggtgcgc
     5521 tacgcgcaac tgatgtcgga tgcctcggag ctggtgcccg tgcacatcga cccgaaccgt
     5581 cccgcccaga acaccctgat gggcggcctc cccgagggca cctacacgct gcagctgccc
     5641 gtgcagcgcc ccgacacccc cgtccccgtc gtggagctgt tcctgaccga ccccgtctga
     5701 ccccacaacc cggtcgcctg gaccgagacc gccgccggcg gtctcctcgg cgacccctcg
     5761 aagaggagga agaaatgaag agaaggacgt tcatcctgtc ggccatggcc ggtgcggcca
     5821 ccgccgtggt cggcgcccag gccgccggtg catcgatgtt cggcgtcggc ccggccgcat
     5881 ccggagtcgt gccgatgctc cctggcgtcg gcccgtactc gcgtgcgtcc tcgctggccg
     5941 agctgcgcgc ggcgatcgcc gccgctcccg agggcgggac gatcatcgtc gcgaacggca
     6001 cctacgttgt gccggaaggg gagcccatcg atctgacggg cgtcggaggc cgctggaaca
     6061 agccgctgat gatcacggcg gagtcgatcg gcggggtcac gttcaccggg gcggagagct
     6121 tccggttcgg cggcacgcac gacctcgtgc tgcgcggctt cgtcttcacc cagtcgacca
     6181 cgttcgagct gccggcggac tgccagtcga tcaccctcag ccgcaacgac ttccagctgg
     6241 ccgacatcga gggcctgctc tgcgtgatgg tgcgcgcgga cgactcggtc gtcgagtaca
     6301 accacttcca caacaagtcg acgctcggcg tcttcctcgg tgtggaaggc gccggcagca
     6361 ccgagatggc gcagaacgtg cacatccacc acaactactt cagcgaccac accttcgcgg
     6421 gggacaacgg cggcgagccg atccgcctgg gcgtgagccc gcgtgcgctg agcagcgcgc
     6481 acgcgatcgt cgagtacaac ctgttcgagc gctgcaacgg cgaccccgag gcgatctcgg
     6541 tcaagtccag cgacaacatc atccggtaca acaccatccg cgacagcggc ggcggcatcg
     6601 tgctgcggca cggcaaccgc aaccgggtgg agtcgaacca catcatccgc ggcctgcgcg
     6661 gcatccgcat ctacggaaac gatcacgtga tcgtgaacaa ctacatcggc gagaccgccg
     6721 acacggcgtt cgtgctgggc gcgggcaccg agcgggatca ctatgagggt gagccggccg
     6781 cctcgcgcag gaagaacgat gcggccgacc gggtgcacat cgcgctgaac acggtctacg
     6841 gcaacgcggg cggcatctcc tgtgagacgc accgccccgt cacgccgatg gactgcgtga
     6901 tcgtcgacaa catcttccag ggcgacgcgg gcacgctgag cgaggtggcg aactcgatcg
     6961 gtttcacctg ggcgggcaac ctgttctggg gtgctgctcc ggacggcgac atgccggcct
     7021 ccggcttcac ccgcgtggac ccgaagcttg cgaaggatgc cgacgacgtg tggcgcatcg
     7081 gttccggcag cgccgcggtg gatgccgcga cgcgtgacca ccgggccgtg gtgcgcgatg
     7141 acatcgacgg tcgtcagcgc ctcggaacgg cggatgtcgg agcccatgag ctgagcatgg
     7201 cggcaccgcg tcgcagcccg ctcgcggcat ccgaggtcgg ccctctctcg cgctaatctg
     7261 gcaagcggtt gccgtgccgc gtactccccc gtgtctcgca gcgcggcgac cgccgtcggt
     7321 gttccacaag cggtgagtgc acgggcatcc gtcccaccac ctgtgggaca cgctgcggtg
     7381 tgtgtcgcag aactggcggg tcggccgcct ggcgacccgc cactgctggg acacgccgtc
     7441 gcgcgggtct cagccgcctc ggccctccgc gctgtgtgcc acaagtggcg ggtgtgcggg
     7501 taaccgtccc accgcttgtg ggaccggatg cggtccgtgt cgcagtagtg gtggctccgg
     7561 cgccccgcga cccgccacta ctgggacacg ccgcggcgca ggtctcggtg ccgcgcgtgt
     7621 ccgagcggcg gagtcaggca gccggacccg tggagccgcg caccaccagt gtcgtctcga
     7681 ggtcgaagcc cacgtacggc gcggcatcgc cggcgacccg tgagagcagc gcggtcatcg
     7741 catggtcgcc ggcctcgcgc agcggcgact tgaccgtcgt gagcgagggc gaggtgaagt
     7801 cggcgccgaa gatgtcgtcg aagccgatca cgctgaggtc ggcggggatg cggatgccgg
     7861 tctcgaccag ctcctgcatc aggccgatcg cgatcaggtc gttgtacgcg accacggctg
     7921 tcgcgccgct gtcgcgggca gcgcgcgccg cgctgcggcc tccggcgacg g
//
LOCUS       DATMSX010000140         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20558,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000140 DATMSX010000000
VERSION     DATMSX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20558"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..458)
                     /locus_tag="VN041_04235"
     CDS             complement(78..458)
                     /locus_tag="VN041_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU28289.1"
                     /translation="MTASRLHLVRHGEVHNPARVLYGRLPDYHLSDAGRAMARAAAEH
                     VQQFGRPVVELRCSPLERTQESAAPFAELFDLAPTLDDRIIEPTNVFEGRRMSRALRD
                     PRNWWHLRRPRPAQARATDHRDGC"
     gene            559..2346
                     /gene="aspS"
                     /locus_tag="VN041_04240"
     CDS             559..2346
                     /gene="aspS"
                     /locus_tag="VN041_04240"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWU28290.1"
                     /translation="MLRTHTAGSLRAEHIGQTVTLTGWVDRRRDHGGVAFIDLRDASG
                     IAQVVIRDEEVAHPLRSEFVLQVTGEVSRRPAGNENPNLPTGEIELIATDVVVLNESA
                     PLPFQVSSGLAETDPIGEEVRLKHRYLDLRRPAPAAAMRLRSDVYKAIRDELHKREFV
                     EVETPTLTRSTPEGARDFLVPARLHPGSWYALPQSPQLFKQLLMVGGIEKYYQIARCY
                     RDEDFRADRQPEFTQLDIEMSFVDQEDVIEMMESLIVAMWKTIDVDVQTPLPRMTYAD
                     AMEKYGSDKPDLRFGLELVEATDYFKDTPFRVFQSEYVGAVLMPGGASQPRKTLDAWQ
                     DWAKQRGARGLAYVLFNEEGTLGGPVAKNLSEAEQAGLAELVGAKPGDCAFFAAGETK
                     ASRALLGAARVEIGRRLELLDPNEFAFTWVVDAPMFEPAGDAIASGDVAVGSGAWTAV
                     HHAFTGPKPEFEDTFDTDPASALAYAYDIVCNGSELGGGSIRIHREDVQKRVFKVMGI
                     SEEQADEKFGFLLDAFKFGAPPHGGIALGMDRVMQHLTKTDSIREVIAFPKSGNGFDP
                     LTEAPGTISPEQRAEAGVDFVPEDEPAEV"
     gene            complement(2454..3281)
                     /locus_tag="VN041_04245"
     CDS             complement(2454..3281)
                     /locus_tag="VN041_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWU28291.1"
                     /translation="MDIGALLGLEQLTWGMLVLVIVAAFGAGWIDAVVGGGGLLQLPA
                     LLLIPGILPVQALATNKLAGLAGTTTSAATYYRRAKPDLRTAVPMALIALAGSFGGAA
                     VAIVLPASAFKPIIVVALLAVAIFTAFRPEMGAATKLRFQGHRHHVAAGATGLLIGFY
                     DGLIGPGTGTFLVITLVALMGYDFLQSSAKAKIVNFATNLGALLLFIPHGAVLWLFGA
                     ILAAANVGGSYLGSRMAISRGSTFIRVVFLIVVVLLIGKLGVDVWNENIAPALASAS"
     gene            3308..3847
                     /locus_tag="VN041_04250"
     CDS             3308..3847
                     /locus_tag="VN041_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28292.1"
                     /translation="MLDALTLPHPLPFRTGDAVLRRASEADADAIIALLADDPVSAAR
                     GDQAKPEDRELYTSGLRAILASPENDLLVAEVDGAIVATFQLTLIPGMARRGALRLLV
                     EAVRVRSDLRSAGLGSALMRWVTDSAAPALDAALVQLTSDAARVDAHRFYERLGFVGS
                     HRGFKYAVASPSTPGATSR"
     gene            3942..5084
                     /locus_tag="VN041_04255"
     CDS             3942..5084
                     /locus_tag="VN041_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28293.1"
                     /translation="MAIITRRTRLGAGIALAATAALLLTSCSGGSTPGGSDGGDAPKS
                     ATATSVKDFGSFADLEAAAKKEGQLNVIALPHDWANYGAILDAFKKKYPEITINEQSP
                     DVSSADEITAAKTNKGLDTAPDVFDLGLAVALSNTDVFAPYKVQTWDDIPAELKESTG
                     LFVGDYGGYMSIGYDSSKFPEPKSLDDLKGAKFKGSVAINGDPTQAGAAFAAIGLATV
                     QSDGALDDFQPGIDYFAELQKAGNFLKVDPTPATVASGETPVVFDWDYLNTAYSKDVK
                     SWKVTVLPGTGYAGYYNQAINKDATHPAAARLWQEFLYSDEGQNLYLAGGARPARMEA
                     MTKAGTIDEKLASALPKAPEKTVVPTEKQSTDAGTLLGEKWAAAIQ"
BASE COUNT          862 a         1827 c         1692 g          733 t
ORIGIN      
        1 tcagcaaatc ctcgacgaga tcgaggacta taaccggatt gattgccagt cgacccagct
       61 gctgcgcgat tggctggtca gcatccgtcc cgatggtccg tggcccgtgc ttgcgcagga
      121 cgcggccggc gcaggtgcca ccagttgcgc gggtcacgca gcgcgcgcga catccgcctc
      181 ccctcgaaca cgttggtcgg ctcgatgatg cgatcgtcga gcgtcggagc gaggtcgaac
      241 agctcggcga aaggcgccgc cgactcctgc gtccgctcca gcggtgagca gcgcaactcg
      301 acgaccggcc gtccgaactg ctgcacgtgc tcggcggcgg cacgcgccat cgcgcggccg
      361 gcgtcgctga ggtggtagtc cggcagccgg ccgtacagca cccgcgcagg gttgtgcact
      421 tcgccgtggc ggacgaggtg gagtcgggat gccgtcacgc acccaccgta tcgcgggacg
      481 cctcgcgccc gtctgcccgc atgaggagcc cccgtagact ggtgcggtcc ctctttcccc
      541 gatcagaagg aatcctccgt gcttcgcaca cacacggcag gctcgctgcg agccgagcac
      601 atcggtcaga ccgtcaccct cacgggctgg gtcgatcgtc gccgcgatca cggaggcgtc
      661 gcgttcatcg atctccggga tgcctcgggc atcgcgcagg tcgtcatccg tgacgaggag
      721 gtcgcacacc cgctccgcag cgagttcgtg ctgcaggtga ccggcgaggt ctcccgccgt
      781 cctgccggca acgagaaccc gaacctcccc accggcgaga tcgagctcat cgcgaccgac
      841 gtggtggtgc tgaacgagtc ggcgccgctg ccctttcagg tctccagcgg actggccgag
      901 accgacccga tcggcgagga ggtgcgtctg aagcaccgct acctcgacct gcgccgcccg
      961 gcacccgccg ccgccatgcg cctgcgctcg gacgtctaca aggcgatccg cgacgagctc
     1021 cacaagcgtg agttcgtcga ggtcgagacc cccacgctga cgcggtcgac ccccgagggc
     1081 gcccgcgact tcctggtgcc ggcccgcctg cacccgggca gctggtacgc gctgccgcag
     1141 tcgccgcagc tgttcaagca gctgctcatg gtgggcggca tcgagaagta ctaccagatc
     1201 gcgcgctgct accgcgacga ggacttccgc gccgaccgcc agcccgagtt cacccagctc
     1261 gacatcgaga tgagcttcgt cgaccaggag gacgtcatcg agatgatgga gtccctcatc
     1321 gtcgcgatgt ggaagaccat cgacgtcgac gtgcagaccc cgctcccgcg gatgacctac
     1381 gccgacgcca tggagaagta cggctccgac aagcccgacc tgcggttcgg cctggagctc
     1441 gtcgaggcca ccgactactt caaggacacc ccgttccggg tgttccagtc cgagtacgtc
     1501 ggcgcggtgc tcatgcccgg cggcgcctcg cagccccgca agacgctgga tgcgtggcag
     1561 gactgggcca aacagcgcgg cgcccgcggc ctggcctacg tgctcttcaa cgaggaaggc
     1621 accctgggcg gccccgtcgc caagaacctc tccgaggccg agcaggcggg cctcgccgag
     1681 ctcgtgggtg cgaagcccgg cgactgcgcc ttcttcgccg cgggtgagac caaggcgagc
     1741 cgtgccctgc tcggcgcggc acgtgtggag atcggccgcc gcctggagct gctcgacccg
     1801 aacgagttcg cgttcacgtg ggtcgtcgac gcccccatgt tcgagccggc cggcgacgcc
     1861 atcgcctccg gcgacgtggc cgtcggctcc ggcgcctgga cggccgtgca ccacgccttc
     1921 acgggcccga agccggagtt cgaggacacg ttcgacaccg acccggcatc cgctctggcc
     1981 tacgcgtacg acatcgtctg caacggctcc gagctgggcg gcggctcgat ccgcatccac
     2041 cgcgaggacg tccagaagcg cgtcttcaag gtgatgggca tcagcgagga gcaggccgac
     2101 gagaagttcg gcttcctgct ggacgccttc aagttcggcg ctcccccgca cggcgggatc
     2161 gcgctgggca tggaccgcgt catgcagcac ctgaccaaga ccgactcgat ccgcgaggtg
     2221 atcgcgttcc cgaagtccgg caacggcttc gacccgctca ccgaggcccc cggcacgatc
     2281 tccccggagc agcgtgccga ggccggcgtc gacttcgtcc cggaggacga gcccgccgag
     2341 gtctgaccct cggtccactc cttcgccgag acccctactt gccgccgaga cccctacgta
     2401 cggttgtcta cacgtagggg tctcggcgcg agctgggggt ctcggcgaca ggctcaggag
     2461 gcggatgcca gggcaggggc gatgttctcg ttccagacgt cgacgcccag cttcccgatg
     2521 agcagcacga cgacgatcag gaacacgacc cggatgaatg tggaaccgcg cgagatcgcc
     2581 atccgcgagc cgagatagct tccgcccaca ttcgccgccg cgaggatcgc gccgaacagc
     2641 cacagcacgg cgccgtgcgg gatgaacagc agcagcgcac cgagattggt ggcgaagttc
     2701 acgatcttcg ccttggcact ggactgcagg aagtcgtagc ccatcagtgc cacgagcgtg
     2761 atcaccagga acgtgccggt gcccggaccg atcagcccgt cgtagaaccc gatcaggagc
     2821 cccgtggcgc cggcggcgac gtgatgccga tgcccctgga accgcagctt cgtggccgcg
     2881 cccatctcgg gccggaacgc cgtgaagatc gccacggcga gcagggcgac gacgatgatc
     2941 ggtttgaagg cggatgccgg gagcacgatc gccacggcgg ctccgccgaa tgatccggcg
     3001 agcgcgatga gcgccatcgg caccgccgtg cgcaggtcgg gtttcgcccg gcggtaatag
     3061 gtggcggcgc tggtcgtggt gcccgcgagg cccgcgagct tgttggtcgc cagcgcctgc
     3121 accggcagga tgcccgggat cagcagcagt gccggcagct gcagcagacc gcccccgccg
     3181 accaccgcgt cgatccaccc cgcgccgaag gccgcgacga tgacgagcac gagcatgccc
     3241 caggtgagct gttcgagtcc gaggagtgct ccgatgtcca tcacgacaag aatgtcagac
     3301 tgatcccatg ctcgacgccc tcaccctgcc gcatccgctg cccttccgca ccggcgacgc
     3361 cgtgctgcgc cgtgcgagcg aggcggatgc cgacgcgatc atcgccctgt tggccgatga
     3421 cccggtcagc gcagcgcgcg gagatcaggc gaagccggag gatcgcgagc tctacacctc
     3481 cggcttgagg gcgatcctgg ccagccccga gaacgacctg ctggtcgccg aggtcgatgg
     3541 ggccatcgtc gccacgttcc agctcactct catccccggt atggctcggc gtggcgcgct
     3601 gcgcctgctg gtggaggcgg tgcgagtgcg cagcgatctc cgctccgcag ggctcggctc
     3661 ggcactgatg cgctgggtga cggattccgc ggcgccggcg ctcgatgcgg ccctcgtgca
     3721 gctgacatcg gatgccgccc gagtcgacgc gcatcgcttc tacgagcgtc tcggtttcgt
     3781 cggctcgcac cggggcttca aatacgcagt cgcgtcaccg tcgacaccgg gtgccacctc
     3841 ccgataacct cccgttaacc ggcgtccccc tgcggggtaa ccggggacgc gtagcgtcca
     3901 gccgtcccga accggcttcg ccggaactcc gagaggaaaa catggcaatc atcacccgcc
     3961 gcacgcgcct gggcgccggc atcgcattgg ccgcgaccgc ggcactgctg ctcacctcct
     4021 gctccggcgg cagcacgccg ggcggctccg acggcggcga cgcccccaag tccgcgaccg
     4081 cgacgtcggt gaaggacttc ggctccttcg cagacctcga ggccgctgcc aagaaggagg
     4141 gtcagctgaa cgtcatcgcc ctcccgcacg actgggcgaa ctacggcgcg atcctggatg
     4201 ccttcaagaa gaagtacccc gagatcacga tcaacgagca gtcgcccgac gtgtccagcg
     4261 ccgacgagat caccgcggcg aagaccaaca agggtctcga caccgcaccc gacgtcttcg
     4321 acctgggcct cgcggtcgca ctgtccaaca ccgacgtctt cgctccctac aaggtgcaga
     4381 cctgggatga catccccgcc gagctgaagg agtccacggg gctcttcgtc ggcgactacg
     4441 gcgggtacat gtcgatcggg tacgactcct cgaagttccc cgagcccaag agcctcgacg
     4501 acctgaaggg cgccaagttc aaggggtccg tcgcgatcaa cggagacccg acgcaggccg
     4561 gcgccgcgtt cgcggcgatc ggcctggcca cggtgcagtc cgacggcgcg ctggacgact
     4621 tccagcccgg catcgactac ttcgccgagc tgcagaaggc gggcaacttc ctcaaggtgg
     4681 acccgacgcc cgcgacggtc gcgagcggcg agaccccggt cgtgttcgac tgggactacc
     4741 tgaacaccgc ctactccaag gacgtcaaga gctggaaggt gacggtgctt cccggcaccg
     4801 gctacgcggg ctactacaac caggccatca acaaggacgc cacgcacccg gcggccgcgc
     4861 gcctgtggca ggagttcctg tacagcgacg agggtcagaa cctgtacctc gccggtgggg
     4921 cccgtccggc acgcatggag gccatgacca aggccggcac catcgacgag aagctcgcct
     4981 ccgcgctgcc gaaggcgccg gagaagaccg tcgtcccgac cgagaagcag agcaccgacg
     5041 cgggcacgct gctcggcgag aagtgggcgg cggcgatcca gtgacgaccg ccgtcacccc
     5101 gggggcggat gcca
//
LOCUS       DATMSX010000141         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_68336,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000141 DATMSX010000000
VERSION     DATMSX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68336"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VN041_04260"
     CDS             <1..403
                     /locus_tag="VN041_04260"
                     /inference="COORDINATES: protein motif:HMM:NF036996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28294.1"
                     /translation="APRIGLVKVSPGLTGGQLAAALTECPAGVVLECYGSGTAPMTVG
                     GLTEVVRAGVDGGSIVLAVTQCADGAVDLGRYAVGSLLADAGAVAGGDMTPESALGKL
                     AALRRTDLSPEQVRALLAQNILGEREDAPTS"
     gene            complement(531..1625)
                     /locus_tag="VN041_04265"
     CDS             complement(531..1625)
                     /locus_tag="VN041_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HWU28295.1"
                     /translation="MAGVPALPMPVRKGRSVSVWVFGVLGFVMLALIAYFAMFLGTGA
                     SVVGLILAFVPLGIVLFGVRLIDRWEPEPKSLVIFALAWGAIAAIGIALLFDLLLTLA
                     AGRPDDFFSAVIQAPLIEEIGKGLGVFLIYVIGRRAFDGPVDGVVYGALVGAGFAFTE
                     NIQYFGVSLVNGGAGELTITFILRGLLSPFAHAMFTALTGFAIGLAARRGATVAGALG
                     AGALGLLGAMALHAFWNGSSMLGDFFTLYLTAQIPLFLGFIFAIVALRRAEARLTQRR
                     LEDYAAAGWFTKEEVVMLATPAGRKAGMQWARGLRGDRRKLMRSFIKDATELAAVRQR
                     AINGHDAYAAADEQSLLARTRATRAHLLAY"
BASE COUNT          294 a          595 c          602 g          221 t
ORIGIN      
        1 cgcgccccgg atcggactcg tgaaggtgtc gccgggcctg accggcggtc agctggcggc
       61 ggcgctgacc gaatgccccg caggcgtcgt gctcgagtgc tacggctcgg gcactgcacc
      121 gatgacggtg ggcggactga ccgaggtcgt gcgcgcgggc gtcgacggcg gcagcatcgt
      181 gctggccgtc acgcagtgcg ccgacggtgc cgtcgacctc gggcgctacg cggtcgggtc
      241 gctgctcgcc gatgcaggcg ccgtcgcggg cggcgacatg acccctgagt ccgcactcgg
      301 caagctcgct gcgctccgcc gcacggacct ctcccccgag caggttcgtg cactgctcgc
      361 gcagaacatc ctcggggagc gcgaagacgc accgacctct tgacttccgt gcacagccgg
      421 tgcaccctgt cagtaggcca actcagcgcc cgggagacta gcaggcatcg ccgcagcacc
      481 gggcgctgag tgctgttcag gggcttagtg ctgttcggga cggcactcgc tcagtacgcc
      541 agcaggtgcg cacgggtcgc cctggtgcgc gccagaagcg actgctcgtc cgcagcagca
      601 taggcgtcgt gcccgttgat cgcacgctga cggacagccg cgagctcggt ggcgtccttg
      661 atgaacgacc gcatcagctt gcggcgatca ccgcgcagcc cgcgggccca ctgcatcccg
      721 gccttgcgtc cggcgggggt cgcaagcatg acgacctcct ccttcgtgaa ccagcccgct
      781 gcggcgtagt cctcgagccg tcgctgcgtg agccgggctt ccgcccgccg gagggcgacg
      841 atcgcgaaga tgaagccgag gaacagcggg atctgcgccg tgaggtagag cgtgaagaag
      901 tcgcccagca tcgacgagcc gttccagaag gcgtgcagcg ccatcgcgcc gagcagtccc
      961 agcgccccgg cgccgagtgc accggcgacg gtcgccccgc ggcgtgccgc gagcccgatg
     1021 gcgaagcccg tgagcgccgt gaacatggcg tgggcgaacg gcgagagaag gccacgcagg
     1081 atgaaggtga tggtcagctc accggcgccg ccgttgacca ggctgacgcc gaagtactgg
     1141 atgttctcgg tgaaggcgaa tcccgcaccc accagcgcgc cgtagacgac gccgtcgacg
     1201 ggaccgtcga acgctcggcg tccgatgacg tagatcagga agacgccgag ccccttgccg
     1261 atctcctcga tcagcggtgc ctggatcacc gcggagaaga agtcgtcggg acggccggct
     1321 gccagggtca ggagcaggtc gaacagcagc gcgatgccga tggccgcgat cgctccccag
     1381 gccagcgcga agatcaccag gctcttcggc tccggctccc agcggtcgat gagccgtacc
     1441 ccgaacagca cgatgccgag ggggacgaag gcgaggatca gaccgacgac cgaggcgccc
     1501 gtgccgagga acatcgcgaa gtaggcgatc agcgcgagca tcacgaagcc cagcacaccg
     1561 aacacccaca ccgagaccga gcgacccttg cgcaccggca tcggcagcgc cggcacaccg
     1621 gccacaggct cgggatcacg tccgggccga cgtagccgat gcggccatcg gcagcgtaca
     1681 cggcgttgta caccgaggtg ccgccggtgg cc
//
LOCUS       DATMSX010000142         9058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20580,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000142 DATMSX010000000
VERSION     DATMSX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20580"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VN041_04270"
     CDS             complement(<1..230)
                     /locus_tag="VN041_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28296.1"
                     /translation="MSAAGSADVVVIGSGIVGLGAAYAAVRRGLSVIVVDRTDAPVGA
                     SIRNFGHLCIGAQTGVARRHADTARELWLQLAR"
     gene            complement(227..1978)
                     /gene="phnE"
                     /locus_tag="VN041_04275"
     CDS             complement(227..1978)
                     /gene="phnE"
                     /locus_tag="VN041_04275"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HWU28297.1"
                     /translation="MTSLLERSGGPSTGSGSQDGADAALQARAPRRRLSPERISASLT
                     LLVIAGLAVVSLRDIDISFAGVADSLGNAERFFTRVGGLSFPEPGELLQQTVLTVGLV
                     LTGTLLAAALSVPIAYLAASNTTPHPALRALARFIGVFTRALPDVVLAMVFVLMFSLG
                     TLPGILAIGIHSIGMISKMFADAIEQIDEGPRLAIRAAGGTRMQEFTTGVLPQVLPSW
                     VATVLHRNDINLRGSVVLGYVGVAGLGLEMSHAFKSLDYGLGLGIALVIFVLCVVMEI
                     ISSAVRVAMLGEKDAGRGMFVRLFSRRSQAAPTGVAVGARRYDTVEQALRRPWTPQRV
                     RNTMAGWIAVAVVVASVVVSDIVWGDLLSFWSKVPAVAAQFWPPDFGNYGFAVIGAAM
                     LDTVKIALAATLITFVLSLVIGSLAARNVAPNRGVQGGFRLLLVIIRGIPELILAIVL
                     IIITGLGPQAGTIALAIGGIGLLGKLIADSFEEVPRGPERALVATGATRLQMYAAATF
                     PQGSRALIGHTFYMLDTNVRAATLLGIVGGGGVGYYLLNAGQGSNYPLVASIVIMILV
                     TVLAVEGLAMWMRKVFA"
     gene            complement(1975..2787)
                     /gene="phnC"
                     /locus_tag="VN041_04280"
     CDS             complement(1975..2787)
                     /gene="phnC"
                     /locus_tag="VN041_04280"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HWU28298.1"
                     /translation="MSTEPVIRIDSLTKRFDSTTALSDVDLTVGRGEIVVLLGLSGSG
                     KSTLLRHIDALEIPTSGSVSVLGEQVSALRGKALRRLRSRVGFIFQQFELVGSLTVLE
                     NVLSGSLAGLRGPRLGLFSYSRAHKLRALGHLDRVGLLDRAYQRADTLSGGQQQRVAI
                     ARALMQNPQVLLADEPVASLDPESSSQVMALIREIAADDGLTVLCSLHQVDLAIDWAD
                     RIVGLRHGQVVLDTDTSGLDKAQVMEIYGRVATSTGEIQAMETELLIAEPVA"
     gene            complement(2882..3808)
                     /locus_tag="VN041_04285"
     CDS             complement(2882..3808)
                     /locus_tag="VN041_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU28299.1"
                     /translation="MKLRALPAVAGAVLLALGLAACSGSAGAAPGSDAPSDAKSGYAI
                     DENTLVFGVVPDSVDTETNYQPLMDYIAKETGKKVEYHESTDYAALIEASIAGKVDVA
                     SFSGFTYITATNNGAKLTPISSIVTKEGQEPGYYSMAIAPKDSAISTIEDFKGKKVCF
                     VDPSSTSGYLFPTYNLIKAGVDPDKDITPVFAGKHDVSVTKTGEGTECEAGFAEDSEV
                     AKSDKVKVIAKTMVPGAPLVYANGLPDDVKKKLVDSLAEITIDDIIKAGITSADSDAF
                     RATFYATKPVDDKYYDVIRDICKQTNAKQCKA"
     gene            3981..4727
                     /locus_tag="VN041_04290"
     CDS             3981..4727
                     /locus_tag="VN041_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28300.1"
                     /translation="MAEAVYSQIADDLRAQISSGSLRPGDDVPTEAELAERWRTSRGP
                     IRNALAALRAEGLIQTHRGRPARVVSKKADQAVDVSVPFTRWARELGVTPGAITQEIS
                     LRRAGAERASALEVSPDDTIVSVIRLRLLDGRPTMLERLSYREDVGRLLLQADLDAIS
                     ITEHLAAHGHPIVGLQHEIDAVAADEQDAALLRVPTGSPILRLSRISRDADGRVFEAS
                     EDRYLSEVVRFTVAASGISADGQYLRAVGG"
     gene            4736..5227
                     /locus_tag="VN041_04295"
     CDS             4736..5227
                     /locus_tag="VN041_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HWU28301.1"
                     /translation="MGIQAAYTLVDEQTLDRLVESAPEHLVDELERLELREGSTVYLD
                     KLWDGLHFLLTGVSASTPIDDSPLSEAVVGVHVFDSDDFIGCTEQDELAAIIAALDAV
                     DVDALLAAADFTAFSEAGLYPRIWDGDDDSLRRELSAAFTTLCQVHRRAAAEGMHLII
                     SIL"
     gene            complement(5500..6642)
                     /locus_tag="VN041_04300"
     CDS             complement(5500..6642)
                     /locus_tag="VN041_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU28302.1"
                     /translation="MPFGRATRDESRILHLGRGERLNTLERVVMLAFLIVVSGADIGS
                     LFFNRDSNPIESGFSIAATAVLILYLWWPPLATILLAAVFAVSFFFGQDPSVLFAAAI
                     AAGFIMRLGSTALVLSYLGGFLVATAIVAYGDDRVPVNVGIYLVVATVAGAVGYALRL
                     ASTRGRTLESRLAESAEQERQAVLAERRWIAGELHDSIAHHLTVVALHVQMLDDPTVS
                     AASQEAIRVAARKAMTDLRFVIELADDGPRETAVQTGDLGEAIDEARTEIEAAGHEVA
                     VTGDPLDERIPRIADIVFARIVRESATNILKHAGAGQVRMEIQVDDEHATLTILSPLP
                     STPRRDMPSSGTGLNRMAERVISASGEFSAGQTGGLWQVYTRMPLS"
     gene            6872..7528
                     /locus_tag="VN041_04305"
     CDS             6872..7528
                     /locus_tag="VN041_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28303.1"
                     /translation="MSEIRVLIVDDDPLVRSALSHFVSRGADVTVIGQAESGREALEF
                     LEHDRPDVVMMDVQMPEMNGIEATAAIAERWPEVRVLAVTTLDGSDTVLPMLSAGASG
                     YLLKDSSAESILEGVREVHSGASSLSPRIASLLVKHVRSTEPAAGAGEALEPLTDRES
                     EVLERLAQGMSNAEIAQSLIVSEGTVKAHLGRIMSKWQVRDRVQILVTAAHAGLVDFR
                     "
     gene            complement(7560..8426)
                     /locus_tag="VN041_04310"
     CDS             complement(7560..8426)
                     /locus_tag="VN041_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWU28304.1"
                     /translation="MPVIAIVLALSAAVAHAAWNVVAHNISRAGVPFLWWGALASTVI
                     WIGVIPFTGGLGTDDLAGFALGVVVSSVLHVLYMSVLQRGYQAGDLSTVYATARGSGP
                     FLSVIIAMLLLGERPSAVALVGVALVIAGVVAVGIVGRTASAGAHRGLDPAVLFGLLT
                     GVMIATYTIWDAHAVREWSLSPVAFMVGTTLAQLPFYSVGVRGRWGAVLDLGRREWRR
                     ILAFGILSPLSYILVLQAITIAPVALIAPLREVSVVLVSLFGAFVLRESRPAWRVAAS
                     VVVVAGIVLLAF"
     gene            8651..>9058
                     /locus_tag="VN041_04315"
     CDS             8651..>9058
                     /locus_tag="VN041_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201699.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HWU28305.1"
                     /translation="MPVSPAALAHASDLADFVAASPSSYHAAEEVARRLQEAGFARLD
                     ETDAWPTQAGGRFVVVRDGAAIGWVVPSDATAITPVHVLGAHTDSPGLKLKPKPTTGS
                     HGWLQAAVEVYGGPLLNSWLDRELRLAGRLALAD"
BASE COUNT         1494 a         3083 c         3146 g         1335 t
ORIGIN      
        1 cgcgcgagct gcagccacag ctcccgtgcc gtgtcggcgt gacgccgggc gacgccggtc
       61 tgcgccccga tgcacaggtg cccgaagttg cggatgctgg ccccgaccgg ggcatccgtc
      121 cggtccacga cgatcacact caggccgcgg cggaccgccg cgtacgcggc gcccagtccg
      181 acgatgcccg agccgatcac gacgacgtcg gcactgccgg cggcgctcat gcgaagacct
      241 tccgcatcca catcgccaga ccctcgaccg cgagcacggt caccaggatc atgatcacga
      301 tgctggccac gagcgggtag ttcgagccct ggccggcgtt gagcaggtag tagccgaccc
      361 cgccgccgcc gacgatgccg agcagcgtcg ccgcgcgcac gttggtgtcg agcatgtaga
      421 acgtgtgccc gatgagcgca cgcgagccct gcgggaacgt cgccgccgca tacatctgca
      481 ggcgggtggc cccggtggcg accagcgcgc gctctggccc tcgtggcacc tcctcgaacg
      541 agtcggcgat gagcttgccc agcaggccga tgccgccgat ggcgagggcg atggtccccg
      601 cctgcgggcc gaggccggtg atgatgatca gcacgatcgc aaggatcagc tcggggatgc
      661 cacggatgat caccagcagc agccggaagc cgccctgcac gccgcggttc ggggcgacgt
      721 tgcgcgccgc gagcgacccg atcaccagcg agagcacgaa cgtgatcagg gtggcggcga
      781 gggcgatctt caccgtgtcg agcatcgccg cgccgatcac cgcgaagccg tagttgccga
      841 agtccggcgg ccagaactgc gccgcgaccg cgggcacctt gctccagaag ctgagcagat
      901 cgccccagac gatgtcgctg accacgacgg atgccacgac cacggcgacc gcgatccagc
      961 cggccatggt gttgcgcacg cgctgcggtg tccacggacg gcgcagcgcc tgctcgacgg
     1021 tgtcgtaccg acgtgcgccc accgcgaccc ccgtcggcgc agcctgcgaa cgacgcgaga
     1081 agagccgcac gaacatgccc ctgccggcat ccttctcgcc cagcatcgcc acgcgcacgg
     1141 cgctggagat gatctccatc accacgcaca gcacgaagat gaccagcgcg ataccgagac
     1201 cgaggccgta gtccagtgac ttgaaggcgt gcgacatctc cagccccagt cccgcgacgc
     1261 ccacatagcc gagcacaacc gagccgcgca ggttgatgtc gttgcggtgc agcaccgtgg
     1321 cgacccagct gggaagcacc tgcggcagca cgccggtggt gaactcctgc atccgcgtgc
     1381 cgccggcagc gcggatggcc agacgcggcc cctcgtcgat ctgctcgatc gcatccgcga
     1441 acatcttcga gatcatgccg atcgagtgga tgccgatggc caggatgccc gggagcgtgc
     1501 cgagcgagaa catgagcacg aacaccatgg ccagcaccac atcgggcagc gcgcgggtga
     1561 agacgccgat gaagcgggcg agcgcgcgca gcgccggatg cggcgtcgtg ttggatgccg
     1621 cgaggtacgc gatcggcacc gagagcgccg cggccagcag ggtgccggtg aggaccagcc
     1681 ccaccgtgag cacggtctgc tgcagcaact cgcccggctc ggggaagctc aggccgccga
     1741 cgcgagtgaa gaagcgctcg gcgttgccga ggctgtcggc cacacccgcg aagctgatgt
     1801 cgatgtcgcg cagcgagacg acggcgaggc ccgcgatcac cagcagggtg aggctcgcgc
     1861 tgatgcgctc ggggctcaga cgccggcgcg gcgcgcgcgc ctgcagcgcg gcatccgcac
     1921 cgtcctggct cccggagccc gtcgaagggc cccccgagcg ctcgagaaga gatgtcatgc
     1981 gaccggctcc gcgatcaaga gctcggtctc catggcctgg atctcgccgg tgctcgtggc
     2041 gacgcgaccg tagatctcca tgacctgcgc cttgtcgagc ccggacgtgt cggtgtcgag
     2101 cacgacctgg ccgtgacgca ggcccacgat gcggtcggcc cagtcgatcg cgaggtcgac
     2161 ctggtgcagg ctgcacagca ccgtgagccc gtcgtcggcg gcgatctcgc ggatgagcgc
     2221 catcacctgc gagctcgact cggggtcgag cgaggcgacc ggctcgtcgg cgagcagcac
     2281 ctgcgggttc tgcatgagcg cgcgggcgat ggccacgcgc tgctgctggc caccggagag
     2341 cgtgtcggcg cgctgatagg cacggtcgag cagccccacg cggtcgaggt ggccgagggc
     2401 gcgcagcttg tgcgcccggg agtacgagaa cagcccgagc cgggggccgc ggagcccggc
     2461 gagcgagccg gacagcacgt tctccagcac ggtgagcgag ccgaccagct cgaactgctg
     2521 gaagatgaag ccgacccggc tgcgcagccg gcgcagcgcc ttgccgcgca gggccgacac
     2581 ctgctcgccg agcacgctga cgctgccgga cgtggggatc tcgagggcgt cgatgtgccg
     2641 cagcagcgtg gacttgcccg agccggagag gcccagcagc acgacgatct caccgcggcc
     2701 gacggtgaga tccacgtcgg agagcgcggt ggtggagtcg aagcgcttgg tgagggaatc
     2761 gatgcggatg accggctcgg tgctcatgac tgtctctttc ggttcggatc tgcggctgcg
     2821 ccgcctcgat cgatgacgac ccgccgggcg cagggtgctg cgcccggcgg gtcggatcag
     2881 atcaggcctt gcactgcttg gcgttggtct gcttgcagat gtcgcggatg acgtcgtagt
     2941 acttgtcgtc gaccggcttg gtggcgtaga aggtcgcgcg gaacgcgtct gagtcggcgc
     3001 tggtgatgcc ggccttgatg atgtcgtcga tggtgatctc ggcgagcgag tcgaccagct
     3061 tcttcttcac gtcgtcgggc aggccgttcg cgtacacgag gggcgcgccg gggaccatgg
     3121 tcttggcgat gaccttgacc ttgtcggact tggcgacctc gctgtcctcc gcgaagcccg
     3181 cctcgcactc cgtgccctca ccggtcttgg tgacgctgac gtcgtgcttg cccgcgaaga
     3241 cgggggtgat gtccttgtcg gggtcgacgc cggccttgat gaggttgtag gtcgggaaca
     3301 ggtaccccga ggtcgaggac gggtccacga agcagacctt cttgcccttg aagtcctcga
     3361 tggtgctgat cgccgagtcc ttgggggcga tggccatcga gtagtagccg ggctcctggc
     3421 cctccttggt gacgatcgag gagatcggcg tgagcttcgc gccgttgttc gtggcggtga
     3481 tgtacgtgaa gcccgagaag gaggcgacgt cgaccttgcc cgcgatcgag gcctcgatga
     3541 gcgcggcgta gtcggtcgac tcgtggtact cgaccttctt gccggtctcc ttggcgatgt
     3601 agtccatcag cggctggtag ttggtctcgg tgtcgaccga gtcggggacg acgccgaaga
     3661 cgagggtgtt ctcatcgatc gcgtagccgg acttcgcatc gctgggagcg tcgcttccgg
     3721 gggccgcgcc ggcggagccg gagcaggcgg cgaggccgag agccagcagg acggcgccgg
     3781 cgacggcagg gagagcacga agcttcatga ggacctttca ggggtgtcgg gaaggtgtcc
     3841 gacagaaact ctgacagccc ggatgccgag aaatgtacct atctcagcac tgttcagcgc
     3901 tcgttcacca aggattcttc cgggttaact ccgcgggaat tcgaccgccc cgagcggtcc
     3961 atgccaagta gtatgcctat gtggctgaag ctgtctactc ccagatcgcc gacgacctcc
     4021 gggcgcagat ctcatcgggg tccctgcgtc ccggggacga cgtgccgacc gaggccgaac
     4081 tggccgagcg gtggcgcacc tcgcgcggac cgatccgcaa cgcgctcgcc gcgctccggg
     4141 ccgagggcct gatccagacc catcgcggac gccctgcgcg cgtggtgtcg aagaaggctg
     4201 atcaggccgt cgacgtgtcg gtgccgttca cgcgctgggc gcgcgagctg ggcgtgacgc
     4261 cgggggcgat cacgcaggag atcagcctgc gccgggccgg ggcggagcgg gcctccgcgc
     4321 tggaggtctc ccccgacgac accatcgtga gcgtgatcag gctgcgcctg ctggacggca
     4381 ggccgaccat gctcgagcgg ctctcctatc gcgaggacgt cggccggctg ctgctgcagg
     4441 ccgacctcga cgcgatctcc atcaccgagc acctcgccgc ccacgggcat ccgatcgtcg
     4501 gcctgcagca tgagatcgac gcggtggccg cggacgagca ggatgccgcg ctgctgcgag
     4561 tgccgaccgg ctcccccatc ctgcggctca gccgcatctc acgggatgcc gacggccgcg
     4621 tcttcgaggc ctccgaggac cgatatctca gcgaggtggt gaggttcacc gtcgccgcgt
     4681 ccgggatctc cgccgacggc cagtatcttc gcgccgtcgg cggttaggac acctgatggg
     4741 catacaggcc gcatacacgc tggtggatga gcagacactc gatcgactcg tcgagtccgc
     4801 tccggagcac ctcgtcgacg agctcgaacg tctggagctg cgcgagggct cgacggtcta
     4861 cctcgacaag ctctgggacg gactgcattt cctcctgacc ggcgtctcgg catccacgcc
     4921 catcgacgac tcccctctca gcgaggccgt cgtcggcgtg cacgtgttcg actcggacga
     4981 cttcatcggc tgcaccgagc aggatgagct cgccgcgatc atcgccgctc tggacgccgt
     5041 cgacgtggat gcgctcctcg ccgccgccga cttcaccgcg ttcagcgagg ccggactgta
     5101 cccgcgcatc tgggacggcg acgacgactc cctgcgacgg gagctctccg cggcgttcac
     5161 gacgctctgc caggttcaca ggcgggccgc ggccgagggg atgcacctga tcatcagcat
     5221 cctctgacgg aggaccgcga gtgcgggcgg acgtcggcca aagagtgaca gcaccaccgc
     5281 gctgaaaggt ccgttaacca gatgcgatcg cgagcgcgtg gtactgaagc ctatgaaaat
     5341 cgcgctcgcg atcgcggcgc tggggaagcg ccttgtttaa gcgctgggga cgcttatctg
     5401 gggagatccc aggatatgac ccgcttccaa ccgatatata ggtccgaaag gcgagaccta
     5461 tgtggacttt cgtctagttc tcgaccggcg ccgatgggct caggaaagcg gcatccgggt
     5521 gtagacctgc cagagaccgc cggtctgccc cgcgctgaac tcgccgctcg cgctgatcac
     5581 gcgctccgcc atgcggttca agccggtgcc gctggagggc atgtcgcgcc gcggtgtcga
     5641 cggcagcggg ctgaggatgg tgagcgtcgc gtgctcgtcg tcgacctgga tctccatccg
     5701 cacctgtccc gctccggcgt gcttgaggat gttggtcgcc gactcccgga cgatgcgggc
     5761 gaacacgatg tccgcgatgc gggggatgcg ctcgtcgagc ggatcgccgg tgacggccac
     5821 ctcatggccg gccgcctcga tctcggtgcg ggcctcgtcg atcgcctcac ccaggtcacc
     5881 ggtctgcacg gcggtttcgc ggggcccgtc atcggcgagc tcgatcacga accggaggtc
     5941 ggtcatcgcc ttgcgggcgg cgacgcggat ggcctcctgg gaggcggcgc tgacggtcgg
     6001 gtcatcgagc atctgcacgt gcagggcgac gacggtgagg tgatgagcga tgctgtcgtg
     6061 cagctccccg gcgatccagc gccgttcggc gaggaccgcc tggcgttcct gctccgcact
     6121 ctccgcgagg cgcgactcga gagtgcgccc tcgggtggac gcgagccgca gggcataacc
     6181 gacggctcct gcgacagtcg ccacgaccag gtagatgccg acgttgacgg gaacacggtc
     6241 gtcgccgtac gcgacgatcg ccgtggcgac gaggaagccg cccaggtacg agaggacaag
     6301 tgccgtcgat cccagccgca tgatgaaccc cgcggcgatg gccgcggcga agaggacgga
     6361 gggatcctgc ccgaagaaga aggagaccgc gaagacggcc gcgagaagga tggtcgcgag
     6421 cggcggccac cacaggtaga ggatgagcac cgcggtcgcg gcgatgctga agcccgactc
     6481 gatcgggttc gagtcgcgat tgaagaagag gctgccgatg tcggcgcctg agacgacgat
     6541 gaggaacgcg agcatgacca cgcgctcgag ggtgttgagg cgttcacctc gaccgagatg
     6601 gagaatgcgg gactcatccc gggtcgctcg accgaacggc atgggtcaat tatctcccat
     6661 cgactgagct acaatcagcc aacagcgccg aaaaggcgag caagttgcca ccagaaggac
     6721 atcggattca cccccagttc taggtctttc gtccaggtac ctattgatct tctcggtgaa
     6781 tgagttgaaa cacatcgcac ctgcgaccag agttgaatgg ccgaaagtcc acatcgcgtc
     6841 cgtgtctctc ccctccggtt aggcttcctc catgtcggaa atacgcgtcc tgatcgtcga
     6901 tgacgacccg ctggtccggt cggcgctctc gcacttcgtc tcacgcggcg ccgacgtcac
     6961 ggtgatcggt caggccgaga gcggccgcga ggccctcgag ttcctcgagc acgaccgtcc
     7021 cgacgtcgtg atgatggacg tgcagatgcc cgagatgaac ggcatcgagg ccaccgccgc
     7081 gatcgccgag cgctggcccg aggtccgcgt gctggccgtc accacgctcg acggcagcga
     7141 caccgtgctg ccgatgctga gcgcgggggc atccggctac ctgctcaagg actccagcgc
     7201 cgagagcatc ctggagggcg tgcgggaggt gcacagcggc gcgagctcgc tgtccccgcg
     7261 catcgcctcg ctgctggtca agcacgtgcg cagcaccgag ccggccgccg gcgcgggcga
     7321 ggcactcgaa cccttgaccg atcgggagtc ggaggtgctg gagcgcctgg cccagggcat
     7381 gtccaacgcc gagatcgccc agtcgctcat cgtctcggag ggcacggtca aggctcacct
     7441 gggccgcatc atgtctaagt ggcaggtgcg tgaccgcgtg cagatcctgg tcaccgccgc
     7501 ccacgccggg ctcgtcgact tccgctgagg cgccgcctcc ggcatccgcc cgctcgccct
     7561 cagaacgcga gcagcacgat ccccgcgacc acgacgacgg atgccgccac ccgccacgca
     7621 ggccgtgact cgcgcagcac gaaggcgccg aacaggctca ccagcaccac gctcacctct
     7681 cgcagcggcg cgatcagcgc cacgggcgcg atcgtgatgg cctgcagcac caggatgtac
     7741 gacagcggcg agaggatgcc gaaggccagg atgcgccgcc attcccgcct gccgagatcg
     7801 agcaccgcac cccagcgccc cctcacgccg acgctgtaga acggcagctg cgcgagcgtc
     7861 gtgcccacca tgaatgccac cggcgacagg ctccactcgc gcaccgcgtg cgcatcccag
     7921 atcgtgtagg tcgcgatcat cacgccggtg agcagaccga acagcaccgc gggatcgagg
     7981 ccgcggtgag caccggcgga tgctgtgcgg ccgacgatgc cgacggccac gacgcccgcg
     8041 atgaccagtg cgacgcccac gagcgcgaca gccgacggcc gttcgccgag cagcagcatc
     8101 gcgatgatca ccgaaaggaa cgggccgctc ccccgcgccg tcgcgtagac ggtcgacagg
     8161 tccccggcct gatagccgcg ctgcagcacc gacatgtaga gcacgtgcag cacggaggag
     8221 accacgacgc cgagggcgaa gccggcgagg tcgtcggtgc cgagtccgcc ggtgaagggg
     8281 atgaccccga tccagatgac cgtgctggcg agggctcccc accacaggaa cggcacgccc
     8341 gcgcggctga tgttgtgtgc gacgacgttc caggcggcgt gcgcgacggc ggcgctgagc
     8401 gccaggacga tggcgatgac cggcatccga gacccttctg cccgtcgcgg gcgcgacgaa
     8461 cgggtcctcc aggcttttgt ccgttcagat gacgcccagc cgacggccgc acgtggcgcc
     8521 gctcggacca gccggactcc gctcgagtgg agcggcgcgt cccggaaccc taggcgctgt
     8581 cccgtccacc ctaacgcgac cggcggggcc tgccgggccg acccggcgcg ccccgactag
     8641 catcgaaggc atgcccgtca gtcctgccgc cctcgcccac gcctctgatc tcgcggattt
     8701 cgtcgccgcc tcgccgtcga gctaccacgc ggccgaggag gtcgcgcggc gactgcagga
     8761 ggccggattc gcacgactcg atgagaccga cgcgtggccg acgcaggccg gcggccggtt
     8821 cgtcgtcgtg cgcgacggcg ccgccatcgg ctgggtcgtg ccgtcggatg ccacggccat
     8881 cacgcccgtg cacgtgctgg gcgcgcacac cgactccccc ggactcaagc tcaagcccaa
     8941 gcccaccacc ggctcacacg gctggctgca ggccgctgtg gaggtgtacg gcggcccgct
     9001 gctgaactcc tggctcgacc gggagctgcg gctcgccggc cgcctggcgc tggcggac
//
LOCUS       DATMSX010000143        11709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_125381,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000143 DATMSX010000000
VERSION     DATMSX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125381"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VN041_04320"
     CDS             <1..527
                     /locus_tag="VN041_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28306.1"
                     /translation="GLTGILSVVGRRMPGLAPVAAVVLAAVVLGVTVPQYLHQRTPYA
                     KDGGADFRFAAQVIGQRAHLGDAVLFGDGPRPSRTSRLAFRLYPEDFAGLQDPQLLAS
                     YDTRAGLWDELASVSAAAPSLRESTVWLLEAKGARTASVDLSALRRAGYTLTAQHHVH
                     RTTAYEFTKGAHHA"
     gene            520..1284
                     /locus_tag="VN041_04325"
     CDS             520..1284
                     /locus_tag="VN041_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWU28307.1"
                     /translation="MPEPTVVILPTYNESETLPGMISRIRAAAPEADLLIVDDSSPDG
                     TGALADAISYADPAVHVLHRTVKQGLGPAYLAGFSWALRHGYDRVVQSDADGSHRPED
                     LPVLLAASEDADLVIGSRWVAGGSAPGWDWRRLLLSRAGSRYAGIMLRLPQRDITGGY
                     RVFRADALDRIVRAGVSSRGYCFQIEMLDRAVSLGSRIVEVPITFDVRGGGESKMSGR
                     IVVEALRQVTAWGVSRRLHPRTCRAAVLAPREHAHV"
     gene            1277..1879
                     /locus_tag="VN041_04330"
     CDS             1277..1879
                     /locus_tag="VN041_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWU28308.1"
                     /translation="MSERGPADGRAGELLTQVVRFGVVGGMGFIVDVAVFNVLRAHTP
                     FGWGWWPVAAKAISTVLAIAVNWIGNRSWTFRDHRRADTGREAAEFLVASLIGGGVSL
                     LCLVVSRDVLGLTSALDDNVSANVVGLLLGSAVRFCAYRWWVFGDRRTPTPDPSGESE
                     MSGMRMPTSAPAMRSDEIVADPPASSATRATIASPSPPPA"
     gene            complement(1819..3108)
                     /locus_tag="VN041_04335"
                     /pseudo
     CDS             complement(1819..3108)
                     /locus_tag="VN041_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
     gene            complement(3105..3797)
                     /locus_tag="VN041_04340"
     CDS             complement(3105..3797)
                     /locus_tag="VN041_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28309.1"
                     /translation="MVTRVLVVDDEPQLADLIARHLRREGYDVTAVGDGIAALLAGGE
                     DEFQLAVVDVNLPGMNGFELASRLRDRDERIGILLLTARDALGDRVRGLDAGADDYLI
                     KPFEFAELFARLRALRRRDHLESHRLEVGDITVAVDRAAAQVDGVDVPLSRREFDLLR
                     AFLERPADTLSREWILSEIWGSPHFASNVVDQYVGYIRRKLADAGSGVRIETVRGVGF
                     RLTSAPANEADG"
     gene            complement(3878..3950)
                     /locus_tag="VN041_04345"
     tRNA            complement(3878..3950)
                     /locus_tag="VN041_04345"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3915..3917),aa:His,seq:gtg)
     gene            complement(3987..4613)
                     /gene="orn"
                     /locus_tag="VN041_04350"
     CDS             complement(3987..4613)
                     /gene="orn"
                     /locus_tag="VN041_04350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HWU28310.1"
                     /translation="MVSASENDRLVWIDCEMTGLDLAVDELVEIAVVITDFELRPLDP
                     GFQIVIKPSEAALANMNEFVTNMHRTSGLIEEIPGGVSLAEAEQRTLDYIKRFVPLER
                     KAPLAGNTIGTDRMFLAKYMPQIDQYLHYRNVDVSSVKELSRRWYPRVFFQAPEKNGG
                     HRALADILESIRELQYYREAVFVAEPGPSSDEARDISTAVVSKFAPNM"
     gene            4660..4732
                     /locus_tag="VN041_04355"
     tRNA            4660..4732
                     /locus_tag="VN041_04355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4693..4695,aa:Arg,seq:tct)
     gene            complement(4770..5768)
                     /locus_tag="VN041_04360"
     CDS             complement(4770..5768)
                     /locus_tag="VN041_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28311.1"
                     /translation="MPTPIDELPDFRPTVLPVPAAGMNPVQRVLDDLGVPWRTSTRDL
                     VARFGSSPHPSYDWDVIPLVGAGAGLEGMIFPIAANAFRTSSTQPPDHFFTCVWVSDD
                     PLENIRIAAESFCALLGPAGLGRSANTVGCAWTAGPAGITLTVWPPSLQSFVSRNRAH
                     ERDPRLVTACRVAFSPGYRQAASEREAHWIDSASVVLRLRDSASKMAAARLTAARPLP
                     TTVEFARDPVPRCVELHGTVSRSEDGAALIVCDRELLVVPLDRVLAVAVRKLSPAKGP
                     GGSWLELVCQDAASDGGETAILLASGRLPDELDATAHRLGERIARRVRIEPAQPDV"
     gene            5858..7363
                     /locus_tag="VN041_04365"
     CDS             5858..7363
                     /locus_tag="VN041_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HWU28312.1"
                     /translation="MTRISCPVLATKVTSAEHAASFIHDGDNVAMSGFTGAGYPKSVP
                     TALAHRIRAAHDRGERFSIGLLTGASTGAEVDGVLAEAEGIHLRTPFQSDPVLRRQIN
                     DGTVDYIDTHLSHLAQQVWFGFYGTMNVAVVEVAAILPNGLLVPSSSVGNNKTWLDLA
                     DKVIIEVNSWQPRAYEGFHDVYYGTRRPPFRTPVQITAATQRIGDPYLKVDPAKVVAV
                     VETHAPDRDNVFSPVDEASKAMADHILDFLRFEVNAGRLPENLLPLQSGVGNVANAVL
                     AGLEESEFEHLTAYTEVIQDEMLRLLESGRFDAISATSLSLSREMAEYFNANIEAFKG
                     RILFRTQEMSNHPEIVRRLGVIAINGMVEADIYGHVNSTNVLGSQMINGIGGSGDFAR
                     NAYLNFFVSPSVAKKGAISAIVPMASHVDHTEHDVHVLVTEQGIADLRGMAPVKRARK
                     IIENCAHPDYRDRLLDYLERAQAQANGRLHMPHLLDEALSWHSAFAKTGRM"
     gene            complement(7381..7644)
                     /locus_tag="VN041_04370"
     CDS             complement(7381..7644)
                     /locus_tag="VN041_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28313.1"
                     /translation="MTAITDVLPAVLWETPIVRSRAERLERAGDHLWRVIAASGTIRG
                     HLRVVADPLGIRYRAERLDLASGAFRLVGEFWDADDAVAALRG"
     gene            7730..8632
                     /locus_tag="VN041_04375"
     CDS             7730..8632
                     /locus_tag="VN041_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU28314.1"
                     /translation="MSAASPYAARLGEIPTERREVEVLGSTTAYWVYGPADEAAPTVI
                     AVHGFRGEHHGLEPVIAYLPDVRVISPDLPGFGETAPIPGREHDLSLYSGWLAEFART
                     VAPDAVILGHSFGSIVSSAAVAGGLKTPQLILINPIGAPALEGSKGVLTRLAVLYYSL
                     GARLPERIGTALLRNPLIVRVMSIAMAKTRDPALRRFIHDQHDTYFSRFSDRDVLHDA
                     FVTSVSHDVRAFAGVIDTPTLLIAAQRDDITPIEAERRLARMFPDAELVEIAEVGHLI
                     HYETPAEAAGAIRRFLRLPVARGR"
     gene            complement(8604..9077)
                     /locus_tag="VN041_04380"
     CDS             complement(8604..9077)
                     /locus_tag="VN041_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU28315.1"
                     /translation="MPALDRVDLELLTALAEDPRTTVVALAERLRLSRNTIQARMARL
                     EQTGVFESYERSYSTEVLGFPLQAFISIGVRQTELPRIIAELARVPEVVQAHGLSGSI
                     DLLARVACRDARHLFDTDARILSIDGVERTETSLAMGEVIPFRVAGLIGLARREA"
     gene            9245..10357
                     /gene="pdhA"
                     /locus_tag="VN041_04385"
     CDS             9245..10357
                     /gene="pdhA"
                     /locus_tag="VN041_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWU28316.1"
                     /translation="MSPHISPITDTESDLELTERIIAPDGTRVTNPQLDRYIEDIDFD
                     ALRGLHRDMVVLRRIDAEGVALQRQGQLGLWAPCNGQEAAQIGTGRALEARDFVFPSY
                     RETGVMYSRGAKPGDYVRMWRGEEGAAYDPAELRIAPLQIIIGAQTLHAVGYAMGIQH
                     DGADEVAVTYFGDGATSQGDVNEAMVFASSYRAPVVFVCQNNHWAISEPVGLQSQFPL
                     AGRAPGFGIPSLRVDGNDVLACMAAMRWALDHARSGKGPAYLEAVTYRMGPHTTADDP
                     KRYRDEAELEQWRRRDPIARLEAHLRAAGEFPQEHIDAVQAQADEMAREMRAACLGMV
                     TRPALAVFDRVYAEPHSGLERERDEYAAYLATFEEA"
     gene            10359..11372
                     /locus_tag="VN041_04390"
     CDS             10359..11372
                     /locus_tag="VN041_04390"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWU28317.1"
                     /translation="MVQMTLGKALNAGLRQAMRDDDKVVLMGEDIGTLGGVFRITDGL
                     KDEFGAKRVIDTPLAESGIVGTAVGLAFRGYRPVIEIQFDGFIYPAFDQIVSQVAKLH
                     YRTQGRIKMPITIRVPWAGGIGAAEHHSESPEAYFVHTAGLRVIAVSDPQDAYRCLQQ
                     AIASDDPVLFFEPKRLYHHKGEVDLEAPLADAPPMGLARVVRQGTDATLITYGAMVST
                     ALDAAAAAEDEGISLEVIDLRSLSPVDYETVTASVRRTGRVVVAHEASREAGVGAEII
                     ASITEFCFEYLEAAPLRVTGHDIPYPPAKMEKFHLPDLDRILDAVDRVMDRPNSLTKG
                     AQQ"
     gene            11369..>11709
                     /locus_tag="VN041_04395"
     CDS             11369..>11709
                     /locus_tag="VN041_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWU28318.1"
                     /translation="MIAEFRLPDLGEGLTEAEVVQWLVKPGDPVTLNQTLAEVETAKA
                     VVELPSPYEGTVSTLHADAGDTVAVGAPLIAFDVAGAEPGAPAPAADEKRADAEEKAQ
                     PNLVGYGAAPAS"
BASE COUNT         1847 a         4061 c         4031 g         1770 t
ORIGIN      
        1 gcgggctgac cggcatcctg tccgtggtcg gacgccggat gcccggtctg gcgccggtcg
       61 ccgccgtggt gctggcagcg gtggtgctgg gcgtcacggt gccgcagtac ctgcatcagc
      121 gcacgccgta cgcgaaagac ggcggagccg acttccgatt cgccgcccag gtcatcgggc
      181 agcgggcgca cctcggcgat gcggtgctgt tcggcgacgg cccgcgaccg tcccgcacgt
      241 cgcggctggc cttccggctc tatccggagg acttcgccgg tctgcaggat ccgcagctgc
      301 tcgcctcgta cgacacccgt gccgggctct gggacgagct cgcctccgtc tccgcagccg
      361 caccgtcgct gcgggagagc accgtctggc tgctggaggc gaagggcgcc cggacggcct
      421 ccgtcgatct gtccgctctg cgtcgcgccg gctacacgct cacggcgcag catcacgtgc
      481 accgcaccac cgcctacgag ttcaccaagg gagcccacca tgcctgagcc gaccgtcgtc
      541 atcctgccga cctacaacga gtccgagacg ctccccggaa tgatctcccg gatccgcgcg
      601 gcggcgcccg aggctgacct gctcatcgtc gacgactcct ccccagacgg caccggggcg
      661 ctcgcagacg cgatctccta cgccgatccc gccgtgcacg tgctgcaccg cacggtcaag
      721 cagggcctcg ggcccgcgta cctcgcgggc ttcagctggg cgttgcgcca cggatacgac
      781 cgcgtggtgc agagcgatgc cgacggctcg caccggccgg aagacctgcc cgtgctgctc
      841 gcggcgtccg aggacgccga tctcgtgatc ggatcgcggt gggtcgccgg ggggtcggca
      901 ccaggttggg actggcggcg cctcctgctc tcgcgcgccg gaagccggta cgcggggatc
      961 atgctgcggc tgccgcagcg cgacatcacc ggcgggtacc gggtgttccg cgcggatgcc
     1021 ctggaccgca tcgtccgcgc cggcgtcagc agccggggat actgcttcca gatcgagatg
     1081 ctcgaccgcg ccgtctccct cggcagtcgc atcgtggagg tgccgatcac gttcgacgtg
     1141 cgcggcggcg gcgagtccaa gatgtcgggc cgcatcgtcg tcgaggccct gcgacaggtg
     1201 actgcctggg gcgtcagccg ccggctgcac ccgcggacct gccgggcggc ggtgctggca
     1261 ccgcgggagc acgcacatgt ctgagcgggg gccggcggac ggtcgcgcgg gggagttgct
     1321 gacccaggtg gtgcgcttcg gcgtcgtggg aggcatgggc ttcatcgtgg acgtcgccgt
     1381 gttcaacgtc ctgcgcgccc acacgccgtt cggctggggg tggtggccgg tcgcggccaa
     1441 ggcgatctcg accgtgctgg cgatcgccgt caactggatc ggcaaccggt cctggacgtt
     1501 ccgggatcac cggcgcgcgg acaccggacg cgaggccgcg gaattcctcg tcgcaagcct
     1561 gatcggcggg ggagtgtcgc tgctgtgcct ggtggtgtcg cgcgacgtgc tcggcctcac
     1621 cagcgcactc gacgacaacg tctcggcgaa cgtcgtcggg ctgctcctcg gctcggccgt
     1681 gcgcttctgc gcctatcgct ggtgggtgtt cggcgatcgg cgcacgccga cgccggatcc
     1741 gtccggggag tcggagatga gcgggatgcg gatgccgacc tcggcgcccg ccatgcgatc
     1801 ggacgagatc gtcgccgatc cgcctgccag ctctgccacg cgcgcgacga tcgccagccc
     1861 cagcccgccc cccgcgtagg aggaggaatc gctgcgcgtg aagcgctcga acgcccggtc
     1921 ggtgacggac ggatcgaacc cggggccgtc gtcggccacg atcagctcga tgtcggtggc
     1981 gttctgacgc agcgtcacca ccacgtgcac cgggtcgccc ttcgacgcac gcagagcgtt
     2041 gtcgatcaga ttgtcgacca cccgggcggc atccgcccgg ccgagccgag catgcgcctc
     2101 aggcgcggag acgtcgaggt cgaggtcgac ggagccgcgc agatcggatg ccggctccgc
     2161 cagtctccag cgcacgtcgt cgacgcgctc ggtgatctcg tcggcgatct cgcgcagcgt
     2221 gctgtcgccc gaggccgagc cggcttcgat ccgggacagc tccagcatgc tctgcgcgag
     2281 gcggatgagc cggttgaggg tggtgcggga ctcggccagc agggtgcgat ccgcgtcggg
     2341 atccgagccg tcgacgagct ccagctgcgc acgcagcacc gcgagcggat tgcgcagctc
     2401 gtgactggca tccgccacca tctgctgctc gcgtgcgctg gcggccctga catcctcgat
     2461 cagctggttg agggtgcggg cgagcgagtc gatctcgtcg ccggcactgc cgacgggcag
     2521 cagctccttc tcgctgcggc gcagcgcgag acgctccgcg ctgcgccgca gccgctccac
     2581 cggacggagt gcggccgtgg cgatcaccca tgcacccgcg gcgaagatca gcgcgctgcc
     2641 gatcaccagg atccacagca gcgcgacgac acccgacacg atgcccgcgg cgatgtcgct
     2701 gtcgcgcgtc accagcaccg tccaggtacc ctgcggcgtc tgcacgacgt ccacgtacac
     2761 gtagtacgtc tcgacgtagt gcacctcgcg cagcatggga cctgggaggg tgagctcgcc
     2821 gatgcgggtc tgcagcgcct tcggcagcgt gttcatcctc gtggtcccgt ccggtgcgac
     2881 gatggccacg tgctggtcga cgccgggctt ctcgaacggc ccggagggat cctcgaggat
     2941 gatgccgcgg tagacctcgg cgatgttgtg gagtacggcc ttctccctcg acaccgcgat
     3001 cgaggagatc tgcgcgtaga cggcgatgca cgcggcgacc accagcagca ccaccaccgc
     3061 ggtggagccg atgatgatcc gggcccggat cgacagccgt cgcctcatcc gtccgcctcg
     3121 ttcgccggtg cggaggtcag cctgaagccg acgccccgca cggtctcgat ccgcacgccg
     3181 gaacccgcat ccgcgagctt gcgccggatg tatcccacgt actggtcgac gacgttggat
     3241 gcgaagtggg gcgaccccca gatctcggac aggatccact cgcggctcag ggtgtcggca
     3301 ggccgctcca ggaaggcgcg cagcaggtca aactcgcggc ggctgagagg gacgtcgact
     3361 ccgtccacct gcgcggcagc gcggtcgacg gcgacggtga tgtcgcccac ctcgaggcga
     3421 tggctctcca ggtggtcccg acgccgcaga gcacgcagcc gggcgaacag ctccgcgaac
     3481 tcgaacggct tgatcaggta gtcgtccgcc ccggcatcca ggcctcgcac ccggtccccg
     3541 agcgcatcgc gcgcggtgag cagcaggatg ccgatgcgct cgtcgcgatc gcgcagccgc
     3601 gaagccagct cgaatccgtt catcccggga aggttcacgt ccacgacggc gagctggaac
     3661 tcgtcctccc cgccggcgag cagcgccgcg atgccgtcgc cgactgcggt gacgtcgtac
     3721 ccctcgcgcc gcagatggcg cgcgatgaga tcggcgagct gcggctcgtc atccacgacc
     3781 agaacccggg tgaccacata cggattctag gtgcgagggc gtcacttccc cgtgatgcga
     3841 agagccccgg cgtcatgacg ccggggctct tcctctgggt ggctaacggg gcttgaaccc
     3901 gcgacccccg cgaccacaac gcggtgctct accaactgag ctatagccac catgtgcccg
     3961 ccggagcagg caactcaacg agtctattac atgttcggag cgaactttga cacgacggcc
     4021 gtggagatat cgcgtgcctc gtccgaactg ggtccgggct cggcgacgaa caccgcttcg
     4081 cggtagtact gcagctcgcg gatcgactcc aggatgtcgg ccagagcgcg gtgcccgccg
     4141 ttcttctcgg gtgcttggaa gaacacgcgc gggtaccagc ggcgggacag ctccttgacg
     4201 ctggacacgt cgacgttgcg atagtgcagg tactggtcga tctgcggcat gtacttggcg
     4261 aggaacatcc ggtcggtgcc gatcgtgttg ccggcgagcg gggccttgcg ctccagcggg
     4321 acgaaccgct tgatgtaatc gagcgtccgc tgctcggcct cggccagcga cacaccgccg
     4381 gggatctcct cgatcagtcc ggaggtgcgg tgcatgttcg tgacgaactc gttcatgttc
     4441 gccagcgccg cctcgctcgg cttgatcacg atctggaatc ccggatcgag cggtcgcagc
     4501 tcgaagtcgg tgatgacgac ggcgatctcg accagctcgt cgaccgcgag gtcgaggccc
     4561 gtcatctcgc agtcgatcca gacgagccga tcgttctcag acgcagaaac catgcctcga
     4621 tcttatcgac gggtccgaca cggtaatctg ggggatctcg cctccttagc tcagtggata
     4681 gagcaacagc cttctaatct gtcggtcgct ggttcgaatc cagcaggggg cacgacgcgg
     4741 cattctctgc cggacccccg caacgacact cagacgtcgg gctgagccgg ttctatccgg
     4801 acccgtcggg cgatccgctc tcccagacgg tgggcggtcg catccagttc atcgggcaac
     4861 cggccggacg cgagcaggat cgcggtctcg ccgccgtcgg atgccgcatc ctggcacacc
     4921 agttccagcc aggagccgcc tgggcccttc gccgggctga gcttccgcac ggccacagcg
     4981 agcacgcgat ccagcgggac gacgagcagc tcccgatcgc agacgatcag tgcggccccg
     5041 tcctcggagc ggctcacggt tccgtgcagc tccacgcagc gcggaaccgg atcgcgggcg
     5101 aattccaccg tggtgggcag cggacgcgca gcggtcagac gcgccgcagc catcttcgaa
     5161 gcactgtccc ggaggcgcag cacgacggag gcgctgtcga tccagtgcgc ctcacgctcg
     5221 gacgccgcct gcctgtagcc gggcgagaac gcgactcggc aggcggtcac cagacgcggg
     5281 tcccgctcgt gtgcgcgatt cctggagacg aacgactgga gtgacggcgg ccagacggtg
     5341 agcgtgatgc ccgccggtcc cgcagtccag gcgcagccga cggtgttggc actgcgaccg
     5401 agacccgcgg gcccgagcag cgcgcagaac gactccgcgg cgatccggat gttctcgaga
     5461 gggtcatccg acacccagac gcaggtgaag aaatggtccg gcggctgcgt gctcgaggtg
     5521 cggaaggcgt tcgcggcgat ggggaagatc atgccctcga gcccagctcc tgctccgacc
     5581 agcgggatca catcccagtc gtaggacgga tgcggcgacg acccgaaccg agcgacgagg
     5641 tcgcgtgtcg acgtccgcca aggcactccc agatcgtcga gcacgcgctg cacggggttc
     5701 atcccggcgg ccggcacggg aagcacggtc ggccggaagt caggcagctc gtcgatcggg
     5761 gtgggcatgc gcccattatc cggtggacgt gccacggcat gcgggcgccg tgccctcggc
     5821 acccggagcg gggccgacgc gatgcgagaa tcggggcatg actcgaatca gctgcccggt
     5881 gctcgcgacg aaggtgacct ccgcggagca cgcggcgtcg ttcatccatg acggcgacaa
     5941 cgtcgcgatg agcggattca ccggagcggg ctacccgaag tccgtcccga cggcactcgc
     6001 gcaccggatc cgcgccgccc atgaccgtgg cgagcggttc agcatcggcc tcctcaccgg
     6061 ggcctcgacc ggcgccgagg tcgacggcgt gctcgccgag gccgagggca tccacctgcg
     6121 cacgcccttc cagtcggacc cggtgctgcg caggcagatc aacgacggaa cggtcgacta
     6181 catcgacacg cacctgtccc atctcgccca gcaggtgtgg ttcggcttct acgggaccat
     6241 gaacgtcgcc gtggtcgagg tggcggcgat ccttcccaac ggcctgctcg tgccgtcgtc
     6301 ctcggtgggc aacaacaaga cgtggctcga cctggccgac aaggtcatca tcgaggtcaa
     6361 cagctggcag ccgcgcgcgt acgagggctt ccacgacgtg tactacggca cccggcgtcc
     6421 tcccttccgc acgccggtgc agatcaccgc ggcgacgcag cgcatcggag acccgtatct
     6481 caaggtcgat ccggccaagg tggtcgccgt cgtcgagacg cacgcacccg atcgcgacaa
     6541 cgtcttctcg ccggtggacg aggcctcgaa ggcgatggcc gaccacatcc tcgacttcct
     6601 gcgattcgag gtgaacgcgg gtcggctgcc cgagaacctg ctgccgctcc agtccggtgt
     6661 cggcaacgtc gcgaacgccg tgctggcggg cctcgaggaa tcggagttcg agcacctgac
     6721 ggcgtacacg gaggtgatcc aggacgagat gctgcggctg ctcgagtcgg gccgcttcga
     6781 tgcgatctcc gccacgtccc tgtcactgtc gcgggagatg gcggagtact tcaacgcgaa
     6841 catcgaggcg ttcaagggaa ggatcctgtt ccgcacgcag gagatgtcca accatcccga
     6901 gatcgtgcgc cgactcggcg tcatcgcgat caacggcatg gtcgaagccg acatctacgg
     6961 gcacgtgaac tcgacgaacg tgctcggcag tcagatgatc aacggcatcg gcgggtcggg
     7021 cgacttcgcc cgcaacgcgt acctgaactt cttcgtgtcc ccgtccgtgg cgaagaaggg
     7081 tgcaatctcg gccatcgtgc cgatggcatc gcatgtcgac cacaccgagc acgacgtgca
     7141 cgtgctggtc accgagcagg gcatcgccga tcttcgcggc atggcgccgg tcaagcgtgc
     7201 gaggaagatc atcgagaact gcgcccatcc cgactaccgg gaccggctgc tggactatct
     7261 ggagcgggcg caggcgcagg cgaacggacg gctgcacatg ccgcatctgc tcgacgaggc
     7321 gctgagctgg cattcggcgt tcgcgaagac cgggcgcatg tgacgctcgg cgctgcgggg
     7381 tcagccccgc agcgccgcca ccgcgtcatc ggcatcccag aactcgccga ccaggcggaa
     7441 ggcgccggat gccaggtcca ggcgctccgc gcgatagcgg atgccgagcg ggtccgccac
     7501 gacgcgcaga tggccgcgga tggttcccga ggcggcgatc acgcgccaga gatgatcgcc
     7561 tgcccgttcg agtcgctcgg cgcgcgagcg gacgatgggc gtctcccaca gtacggcggg
     7621 caggacatcg gtgatcgcgg tcatggctgt tcgcctcctt catccgggaa ctgctctcct
     7681 tcgaacataa ggacggccac cgacatcggc gtcccgtaga ctcgtggcca tgtccgcagc
     7741 ctcgccgtat gccgcccgac tcggggagat ccccaccgag cgacgcgagg tcgaggtgct
     7801 gggatccacc accgcctact gggtctacgg tcctgccgac gaggcggctc ccaccgtcat
     7861 cgccgtgcac ggcttccgcg gcgagcacca cggcctggag ccggtgatcg cctatcttcc
     7921 cgacgtgcgc gtgatctcac ccgacctgcc cggcttcggc gagaccgcgc cgatccccgg
     7981 gcgggagcac gacctctcgc tgtactccgg ctggctggcg gagttcgccc gcaccgtggc
     8041 tccggatgcc gtgatcctgg ggcactcctt cggatcgatc gtctcgtcag cggctgtcgc
     8101 cggcgggttg aagacgccgc agctgatcct gatcaatccg atcggcgcac ccgcgctgga
     8161 ggggtcgaag ggcgtgctga cgaggctcgc agtgctttac tactcgctcg gcgcgcggct
     8221 gcccgagagg atcggcaccg ccctgctgcg caatccgctg atcgtgcgcg tgatgagcat
     8281 cgcgatggcc aagaccaggg accccgcact gcggcgcttc atccacgatc agcacgacac
     8341 ctacttctct cgcttctcgg accgggacgt gctgcacgat gcgttcgtca cgagcgtctc
     8401 gcacgacgtg cgcgcgttcg ccggcgtgat cgacacgccc acgctcctca tcgcggcgca
     8461 gcgtgacgac atcacgccca ttgaggccga acggcgtctg gcgcggatgt tccccgacgc
     8521 cgagctggtg gagatcgccg aggtcgggca cctgatccac tacgagacgc ccgccgaggc
     8581 cgcgggcgcc atccgtcgct tcctcaggct tcccgtcgcg cgaggccgat gagccctgcc
     8641 acccggaagg ggatgacctc gcccatggcg agcgaggtct cggtgcgctc gaccccgtcg
     8701 atcgagagga tgcgcgcgtc cgtgtcgaac agatggcggg cgtcgcggca ggccacacgg
     8761 gcgagcaggt cgatcgaccc gctcaggccg tgcgcctgca cgacctcggg cacgcgcgcg
     8821 agctcggcga tgatccgggg cagctcggtc tgccgcacac cgatgctgat gaaggcctgc
     8881 agggggaagc ccagcacctc ggtcgagtac gagcgctcgt aggactcgaa cacacccgtc
     8941 tgctcgagcc gggccattct ggcctggatc gtgttgcgcg aaaggcggag ccgctcggcg
     9001 agagcgacca cggtggtgcg cggatcctcc gcgagtgcgg tgagcagttc gagatcgacg
     9061 cgatcgaggg ctggcatagt gccacacgat atcaggatca tgagggcgcc ttctgtgcaa
     9121 catgctcaac tggtgcagac atgcttgagc gaggtgtcag gcggacgtac ccttcaggta
     9181 agccgacgga gtcgtcggat cagccgtgca cgccccacaa gggccccacg tcaggaggac
     9241 gccgatgtca ccgcacatct caccgatcac cgatacggag agcgatctcg agctcacgga
     9301 gcgcatcatc gctccggatg ggacgcgggt gaccaacccg cagctcgacc gctacatcga
     9361 ggacatcgac ttcgacgcgc tgcgcggtct gcatcgcgac atggtggtgc tgcgtcgtat
     9421 cgatgccgag ggcgtcgcgc tgcagcgcca ggggcagctc ggcctgtggg cgccgtgcaa
     9481 cggtcaggag gccgcgcaga tcggcaccgg ccgggcgctc gaggcgcgcg acttcgtctt
     9541 ccccagctac cgcgagaccg gcgtgatgta cagccgcggc gcgaagcccg gcgactacgt
     9601 gcgcatgtgg cgcggtgagg agggggccgc ctacgacccc gcagaactgc gcatcgcccc
     9661 gctgcagatc atcatcggcg cacagaccct gcatgccgtg ggctacgcca tgggcatcca
     9721 gcacgacggt gccgacgagg tcgccgtgac ctacttcggc gacggtgcca cgagccaggg
     9781 cgacgtgaac gaggcgatgg tgttcgcctc gtcctatcgc gccccggtgg tgttcgtctg
     9841 ccagaacaac cactgggcga tctccgagcc ggtcgggctg cagtcgcagt tcccgctcgc
     9901 gggtcgcgcc cctggtttcg gcatcccgag cctccgtgtg gacggcaacg acgtgctcgc
     9961 gtgcatggcc gccatgcgct gggccctcga ccatgcgcgc tccggcaagg gacccgccta
    10021 cctcgaggcc gtcacgtacc ggatgggccc gcacaccacg gccgacgacc ccaagcgcta
    10081 ccgcgacgag gccgagctcg agcagtggcg tcgccgcgat ccgatcgcgc ggctggaggc
    10141 gcacctgcgt gccgccgggg agttcccgca ggagcacatc gacgccgtgc aggcgcaggc
    10201 cgacgagatg gcacgcgaga tgcgcgccgc ctgcctgggc atggtcacac gccccgctct
    10261 cgcggtcttc gaccgcgtct acgcggagcc gcactccggc ctggagcgcg agcgcgacga
    10321 gtacgccgcg tacctcgcca ccttcgagga ggcgtgacat ggtgcagatg actctgggca
    10381 aggccctcaa cgccggcctc cgccaggcga tgcgcgacga cgacaaggtc gtgctgatgg
    10441 gggaggacat cggcacgctc ggtggcgtct tccgcatcac cgacggactg aaggacgagt
    10501 tcggggccaa gcgcgtcatc gacacgccgc tggccgagtc cggcatcgtc ggcaccgccg
    10561 tcggcctggc cttccgcggc taccgcccgg tgatcgagat ccagttcgac ggtttcatct
    10621 acccggcgtt cgatcagatc gtctcgcagg tcgcgaagct ccactaccgt acgcagggcc
    10681 ggatcaagat gcccatcacg atccgcgtgc cgtgggccgg gggcatcggc gcggccgagc
    10741 accactccga atcgcccgag gcgtacttcg tgcacacggc cgggctgcgc gtgatcgcgg
    10801 tctccgaccc gcaggacgcg tatcgctgcc tgcagcaggc catcgcgagc gacgaccccg
    10861 tgctgttctt cgagccgaag cggctctacc accacaaggg cgaggtcgat ctggaggccc
    10921 cgctcgccga cgccccgccg atggggctcg cccgggtggt gcggcagggc acggacgcca
    10981 cgctcatcac ctacggcgcc atggtgagca cggcgctcga cgccgccgct gcggctgagg
    11041 acgaggggat ctcgctcgag gtcatcgacc tgcggtccct ctcgccggtc gactacgaga
    11101 ccgtgacggc atccgtgcgc cgcaccggac gtgtggtggt cgcgcacgag gcgtcccgcg
    11161 aggcgggcgt cggcgccgag atcatcgcca gcatcacgga gttctgcttc gaatacctgg
    11221 aggccgcgcc gctgcgcgtg accgggcacg acatcccata cccgcccgcg aagatggaga
    11281 agttccacct ccccgacctc gaccgcatcc tggatgccgt cgatcgggtg atggaccggc
    11341 cgaacagcct cacgaagggg gcgcagcagt gattgcggaa ttccgcctgc ccgacctcgg
    11401 cgagggactg accgaggccg aggtcgtgca gtggctggtc aagcccggcg atccggtgac
    11461 gttgaaccag acgctggctg aggtggagac cgccaaggcc gtcgtcgagc tgccgtcgcc
    11521 gtatgagggg acggtatcga cgctgcacgc ggatgcaggt gacacggtcg ccgtcggcgc
    11581 gccgctgatc gcattcgatg tggccggcgc cgagccgggg gctcccgcgc ccgcggccga
    11641 tgagaagcgg gcggatgccg aggagaaggc gcagccgaac ctggtcggat acggtgccgc
    11701 gccggcatc
//
LOCUS       DATMSX010000144         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_87362,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000144 DATMSX010000000
VERSION     DATMSX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87362"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2146)
                     /locus_tag="VN041_04400"
     rRNA            complement(<1..>2146)
                     /locus_tag="VN041_04400"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          465 a          635 c          446 g          600 t
ORIGIN      
        1 cgacgttctg aacccagctc gcgtgccact ttaatgggcg aacagcccaa cccttgggac
       61 cttctccagc cccaggatgt gacgagccga catcgaggtg ccaaaccccc ccgtcgatat
      121 gagctcttgg gggagatcag cctgttatcc ccggcgtacc ttttatcctt tgagcgatgg
      181 cccttccatg cggaaccacc ggatcactat gctctacttt cgtacctgat cgacctgtat
      241 gtctctcagt caagctccct tatgccattg cactctgcgc acggttacca agcgtgctga
      301 gggaaccttt agaagcctcc gttactcttt tggaggcgac caccccagtc aaactaccca
      361 ccaagcactg tcccccacat tgcggggtta ggcctcagat aaacaaaggg ttgtatttca
      421 acaatgactc cacaacgcct ggcgacgccg cttcatagtc tccaacctat cctacacatc
      481 atttatccaa ggtcaatact aagctatagt aaaggtgcac agggtctttt cgtcccactg
      541 cgggtaaacg gcatcttcac cgttactaca atttcaccga gctcatggct gagacagtgt
      601 ccagatcgtt acaccattcg tgcaggtcgg aacttacccg acaaggaatt tcgctacctt
      661 aggaccgtta tagttacggc cgccgtttac tggggcttca attcaatgct tctccgaaga
      721 taacatctcc tcttaacctt ccagcaccgg gcaggtgtca ggccctatac ttcatcttac
      781 gattttgcag agccctgtgt ttttgataaa cagtcgcctg gacctcttca ctgcggcccc
      841 gattgctcgg ggcgaccttt ctcccgaagt tacaggtcta ttttgcctaa ttccttagcc
      901 atgaatctct cgagcacctt aggattctct cctcaactac ctgtgtcggt ttacggtact
      961 ggttcttatt gcctgaagtt tagaggtttt tcttggaagc ccttaggcgc actatctctt
     1021 tgtccgaaga ctccgagtac tatcgcattt cgccatcttc tacggatttg cctatagaag
     1081 atatagctag gtgctttaac ggactattcc gtcagtccgc ggcgctttca tcactccgtc
     1141 accccatcac agcaataaga agtacgggaa tattaacccg ttggccatcg actgcccctt
     1201 tcgggttcgc cttaggtcca gactaaccca cagctgatta gcatagctgt ggaaacctta
     1261 gtttttcggt gtgcgggttt ctcgcccgca ttatcgttac ttatgcctac attttctttt
     1321 ctgaccggtc cagcatacct tacgatacac cttcagccct gtcagaatgc tcccctacca
     1381 cttgcacata catgcaaatc catagcttcg gtaatatgct tatgcccgat tattatccat
     1441 gctcgtccgc tcgactagtg agctgttacg cactctttaa atgaatggct gcttccaagc
     1501 caacatccta gctgtctggg cagacaaacc tcgttctttc aacttagcat atatttgggg
     1561 accttagctg atggtctggg ttctttccct ctcggacttg gaccttagca cccaagccct
     1621 cactgctggt aaacattata tagcattcgg agtttgtcag gaattggtag gcggtgaagc
     1681 ccccgcatcc aatcagtagc tctacctcta tataactgtc atcagcgctg cacctaaatg
     1741 catttcgggg agtacgagct atttccgagt ttgattggcc tttcacccct acccacaggt
     1801 catccgaaga cttttcaacg tcaaccggtt cggtcctcca ctgtgtgtta ccacagcttc
     1861 aacctgccca tgggtagatc acacggtttc gcgtctaaca ctactgacta aagcgcccta
     1921 ttcagactcg ctttcgctgc ggatccatgg cttaaccact tatccttgcc agcagcgtta
     1981 actcgtaggc tcattatgca aaaggcacgc cgtcacccca cgaaagggct ccgaccgctt
     2041 gtaggcgcat ggtttcagga tctatttcac tccgttattc acggttcttt tcacctttcc
     2101 ctcacggtac tggttcacta tcggtctctc aggagtattt agcctt
//
LOCUS       DATMSX010000145         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_125427,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000145 DATMSX010000000
VERSION     DATMSX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125427"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VN041_04405"
     CDS             <1..1173
                     /locus_tag="VN041_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWU28319.1"
                     /translation="LLAAALVGGVAGFGGSAIGGSLNGTPVSQTATGPQTVTVNNPGS
                     VNQTTAIAADVLPSVVTINVAGSQESGSGSGVILSKDGYVMTNTHVVTLGGAVADPTI
                     TVTTSDGHIYSATVVGTDPIYDLAVIKLKDATGLKPIEFGNSAKLNVGDAAVAVGAPL
                     GLSNSVTDGIVSALNRSIQVASSAVPKNSDSQQNGDGGNGNGNPFQFDLPGQSQSQQP
                     QQSISIAVIQTDAAINPGNSGGALVDSQGRLIGVNVAIATAGQSSSSSSEPGSIGVGF
                     AIPANIAKRVSTEIIENGSATHGLLGAQVQDAASLKDATIAGAVIAKPTAGGAAEKAG
                     LQSGDIITEFDGLPIGSATDLTAQVRAEAAGSTVKITYVREGKTYTTEATLGTLKL"
     gene            1226..>1858
                     /locus_tag="VN041_04410"
     CDS             1226..>1858
                     /locus_tag="VN041_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWU28320.1"
                     /translation="MASFSFGTGNAAKLLRLPLYAAGRIGTLIVPRGRTWVFGCGAGI
                     GDGALALLREAQAQGHRTLWLVRSPREQADARALGIRTRRRDGIRGWWATARAEVIVV
                     THGLGDVNRYGSTDGFVVQLWHGIPLKRIGLDSPVTAQVPARLPGAALLRRLLTVAYR
                     GAAQRIRVLPAASHRSRGRLESAFGLGDDRVVVTGEPRVDVLSAGTPEGRR"
BASE COUNT          290 a          703 c          611 g          254 t
ORIGIN      
        1 ctgctcgccg cggccctcgt gggcggcgtg gcgggcttcg gtggcagcgc catcggcggt
       61 tccctgaacg gcacgccggt ctcgcagacc gcgaccggcc cgcagaccgt cacggtgaac
      121 aaccccggct ccgtgaacca gaccaccgcg atcgcggccg acgtgctccc ctcggtcgtg
      181 acgatcaacg tcgccggctc ccaggagtcg ggcagcggat cgggagtcat cctgtccaag
      241 gacggctacg tcatgaccaa cacgcacgtc gtcaccctcg gcggcgccgt cgcggacccc
      301 acgatcacgg tgaccacctc cgacggtcac atctactcgg ccacggtcgt cggcaccgac
      361 ccgatctacg acctcgcggt catcaagctg aaggatgcga ccggcctcaa gcccatcgag
      421 ttcggcaact ccgccaagct caacgtgggc gacgcggccg tcgcggtcgg cgccccgctc
      481 ggtctgtcga actcggtcac cgacggcatc gtcagcgccc tgaaccgcag catccaggtc
      541 gcgtcctcgg ccgtgccgaa gaactccgac tcgcagcaga acggcgatgg cggaaacggc
      601 aacggcaacc cgttccagtt cgatctgccc ggccagagcc agagccagca gccgcagcag
      661 tcgatctcga tcgcggtcat ccagaccgac gcggcgatca accccggaaa ctccggcggc
      721 gccctcgtcg acagccaggg ccgcctgatc ggcgtcaacg tcgccatcgc gaccgcgggc
      781 cagtcctcct cctcgtccag tgagcccggc tccatcggcg tcggtttcgc catcccggcg
      841 aacatcgcca agcgggtctc caccgagatc atcgagaacg gctccgccac gcacggcctg
      901 ctcggtgctc aggtgcagga tgccgcgtcg ctgaaggacg cgaccatcgc cggagccgtg
      961 atcgccaagc ccaccgcggg cggggcggcg gagaaggccg ggctgcagtc cggcgacatc
     1021 atcacggagt tcgacgggct gcccatcggc agcgccaccg acctcaccgc ccaggtgcgc
     1081 gccgaagccg ccggatccac cgtgaagatc acgtacgtcc gcgagggcaa gacctacacc
     1141 accgaggcca cgctgggcac gctcaagctc tgagcccctc agtccacggt cagttcagga
     1201 cgccaccccg cgataggctc gcggggtggc gtccttctcg ttcggaaccg gcaacgcggc
     1261 caaactgctc cgcctgccgt tgtatgccgc gggacgcata ggaaccctga tcgtcccgcg
     1321 cggacgcacc tgggtgttcg ggtgcggggc aggaatcggc gacggcgccc tcgcactgct
     1381 gcgcgaggct caggcccaag ggcaccgcac gctctggctg gtgcgctcgc cgcgagagca
     1441 ggcggatgcc agagccctgg gcatccgcac gcgtcgccgc gacggcatcc gcggatggtg
     1501 ggcgaccgcc cgcgccgaag tcatcgtcgt cacccacggc ctgggggatg tcaaccgcta
     1561 cggcagcacg gacggcttcg tcgtgcagct ctggcacggc atccctctga agcgcatcgg
     1621 cctggactcg ccggtcaccg cgcaggtgcc tgcacgcctc ccgggcgcgg ccctgctgcg
     1681 ccggctgctg accgtcgcct atcgtggagc ggcgcagcgg atccgcgtgc tcccggccgc
     1741 ttcccaccgc tctcgcggtc gcctcgaatc cgccttcggt ctcggcgacg accgggtggt
     1801 cgtcacgggg gagccgcgcg tggacgtgct ctccgcggga acgccggagg gccggcgt
//
LOCUS       DATMSX010000146         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_39954,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000146 DATMSX010000000
VERSION     DATMSX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39954"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1171
                     /locus_tag="VN041_04415"
     CDS             224..1171
                     /locus_tag="VN041_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28321.1"
                     /translation="MWFLLRRVLVSLVVLLGISLVVFLMLNLISDSPGRAALGQQASQ
                     AAVDAFNREHGYDRPVLIQYVDYLLQLLRGDLGRSYKLNEEVGTLLLQNAPRSAMLSL
                     AGLVLALVIAIPLGILQAVRRGRFIDRAATSISYLLYATPSFLIALVLIGIFSQTIPL
                     FPAEASQSSSPWVVFTDPRSMALPLITLAITNVVVFAQYQRSAALEQLGMDYIRVARA
                     KGASERIVLTRHLLRNAITPIITLVGVLIPTLLAGNLIVEAVFNYPGLGLLFLNSLHR
                     EDYPVLLAYTLIGGALTVIGNLVADLVVARADRRIELGR"
     gene            1168..2067
                     /locus_tag="VN041_04420"
     CDS             1168..2067
                     /locus_tag="VN041_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28322.1"
                     /translation="MSTARAEVAAEELPSLATRNPISRSAVLRSLWRSRTGRTGAVLL
                     VVLVLFCYLGPLIHPTNQTDVDLLNAALPPGDGFPLGTDSNGFDVLGRLMQGGQVSLQ
                     IGLLAAVFATTLGTIYGAVAGLVGGVVDGIMMRVVDVLLSIPFLFFVLIIAARFTATP
                     LTLSLVIGGFSWLVAARLIRGEVLSLRVREFVSAARLMGASNRRLIFSHLIPNTFGVI
                     IVNLTFQVADSIIVLAALGYLGFGLSYPVTDWGSQLSSGVSFLAADYWWLIYPVGGFI
                     VLTVLALNLLGDALRDAIEHRSP"
     gene            2252..4108
                     /locus_tag="VN041_04425"
     CDS             2252..4108
                     /locus_tag="VN041_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28323.1"
                     /translation="MSQVPRRRGRLLLALATAGVLFITGCTASGAPVDATSYKDLPAE
                     SGTPVDGGVVTIALTPGLTPNYIYPYPPAATGGTVIGYDLMWRALYRTSLEGEPFDAA
                     TSLAEEPVISDDGHTATIPLKDHSWSNGKPVTADDVVFTFDLLKAAIAESPANWAFYT
                     PGQFPDGVTVTAADARTAVFSFEKPYNPSYLVSLLQLLYIMPSADWSIAATGGPVLDY
                     TQPENATAIYHYLTGQSEDQSTFVTNPLWQIVNGPYQLSAFEPTTGSFSLTPNKKYSG
                     PGDSRIERVDFKAFTSPSAVYNQFAAGELTTGRLDASFASKIDDLRKKGYNVYGAPSP
                     ARFDGLVINFDNKVDNFDKIIGQLYVRQAMQHLIDQPGYIKSRGIHSGTATANYSTGG
                     LNSAYPPEFGDKATYPYDVKAAEKLLTDHGWKVDAEKGTTCVRPGTGDDQCGEGIPKG
                     QKITFTIASANDPAFVGARDLAFVSQLKKVGIDAKVVTKSLNYMYENYGNSYAPDNAD
                     KWAMQDFGAFVQTSYPTSNNAFNTGGSFNLGSYSNPEADKLIEASTFGADPKALSAEV
                     TMLSKDLPVLFFPTPDNLIVWKKDLSGPPSTFRSMLKFVYSPEQWYFTEKQK"
     gene            4119..>4584
                     /locus_tag="VN041_04430"
     CDS             4119..>4584
                     /locus_tag="VN041_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HWU28324.1"
                     /translation="MSAQIAKRVTPIPTVDPARPGFPAPEAIGALIGDASVVALGEAA
                     HSVVEFTALGDALLRTLVQDHGVTAFVMESGFAEGLLIDEWIHGGPGDVEDIARAGIT
                     YGFGESDGIRRQLIWMREWNAAGGDVRFYGMDLPGSSTSPGTAVRACLARVAP"
BASE COUNT          728 a         1648 c         1465 g          743 t
ORIGIN      
        1 cgcggcgaac aggcgtgccg gccagagcac tccgttgaag accaggaccg cgaggatgcc
       61 gagggccgcg acggtgcccg ctggccaccg acctgtgcgc ccgcgtggag cccgtgctgg
      121 aggacgtcgg cgacgaccac gcggtggcct gccacttccc gctgcagccg aagctccgcg
      181 agacgacgac ggccgaggcc ctgacggcgg gggaggcgcg ctgatgtggt tcctgctgag
      241 acgcgtgctc gtgtcgctcg tcgtgctcct gggcatctcg ctggtcgtgt tcctgatgct
      301 gaacctgatc tccgacagtc ccggccgggc cgccctcggg cagcaggcct cgcaggccgc
      361 cgtcgacgcg ttcaaccgcg agcacggcta cgaccggccc gtactgatcc agtacgtcga
      421 ctacctgctc cagctgctgc gcggcgacct cggacgctcc tacaagctca atgaggaggt
      481 gggcacgctg ctgctgcaga acgccccgcg cagcgcgatg ctgtcgctgg ctggtctcgt
      541 cctcgccctc gtgatcgcga tcccgctggg catcctgcag gcggtgcgcc gcggccggtt
      601 catcgatcgc gccgcgacct cgatctcgta cctgctgtac gcgacgccct ccttcctgat
      661 cgcgctggtg ctgatcggca tcttcagtca gaccatcccg ctgttccccg ccgaggcatc
      721 ccaatccagc tcgccctggg tggtcttcac ggatccgcgc tccatggccc tgccgctgat
      781 caccctggcg atcacgaacg tcgtcgtgtt cgcgcagtac cagcgctccg ccgcgctcga
      841 gcagctgggg atggactaca tccgcgtggc gcgcgccaag ggtgcatccg agcgcatcgt
      901 gctcacccgc cacctgctgc gcaacgccat caccccgatc atcacgctcg tcggcgtgct
      961 gatccccacc ctgctcgccg ggaacctgat cgtcgaggcg gtcttcaatt acccgggact
     1021 cggcctgctg ttcctgaaca gccttcatcg agaggactat ccggtgctgc tcgcgtacac
     1081 gctcatcggc ggggccctga ccgtcatcgg caacctcgtc gccgacctcg tcgtcgcccg
     1141 cgccgaccgg cggatcgagc tgggccgatg agcacggcac gcgcagaggt cgccgccgag
     1201 gagctgccct ccctcgcgac gcgcaacccc atctcgcgct ccgcggtgct gaggtcgctc
     1261 tggcgcagcc ggaccggccg cacgggcgcc gtcctgctcg tcgtactggt gttgttctgc
     1321 tacctggggc cactcatcca tcccacgaac cagaccgacg tggacctgct caacgcggcg
     1381 ctcccgccgg gggacggctt cccactcggc accgacagca acggtttcga cgtgctcggc
     1441 cggctcatgc agggcggcca ggtgtcgctg cagatcggcc tgctcgccgc cgtcttcgcg
     1501 acaaccctgg gcacgatcta cggcgcggtc gccgggctgg tcggcggggt cgtcgacggg
     1561 atcatgatgc gggtcgtcga cgtgctgctg tcgattccct tcctgttctt cgtattgatc
     1621 atcgccgcgc gcttcacggc gacgccgttg acgctgagcc tggtcatcgg cgggttctcc
     1681 tggctggtcg ccgctcggct gatccgcggt gaggtgctgt cgctgcgggt gcgagagttc
     1741 gtctcggcgg ccaggctgat gggtgcgagc aaccggcgcc tgatcttcag ccacctcatc
     1801 ccgaacacct tcggcgtgat catcgtcaac ctcaccttcc aggtggcgga ctcgatcatc
     1861 gtgctcgcgg cgctggggta tctgggcttc ggcctgagct acccggtcac cgactggggc
     1921 agccagctct ccagcggggt ctcgttcctg gccgcggact actggtggct gatctacccg
     1981 gtcggcggat tcatcgtgct cacggtgctc gcgctcaacc tgctcggcga cgccctccgg
     2041 gacgccatcg agcaccgctc gccctgacac ctggcccgct cggagccctg tgtcggaccc
     2101 gacggatcgc ggctcacgaa tctgtcggga tccctgaacg gggcgcggtg cggatgcatc
     2161 acgctcggag catcggagtt ccacatcacc cgacggaccc tccgaccccc atcccgaaca
     2221 tcctgaacat ctcgaacaag gagcacagct catgtcgcag gttccgcgga ggcgaggacg
     2281 cctccttctc gcactggcca cggccggtgt gctgttcatc accggctgca cggcatccgg
     2341 tgcgccggtc gacgcgacgt cgtacaagga tcttcccgcc gagagcggca cccccgtcga
     2401 cggcggtgtg gtgaccatcg ccctcacgcc cggcctcacc cccaactaca tctacccgta
     2461 cccgccggcc gccaccggcg gcacggtcat cggctacgac ctgatgtggc gggcgctgta
     2521 ccgcaccagc ctggagggcg aacccttcga cgcggcgacc agcctggccg aggagccggt
     2581 gatcagcgac gacggccaca ccgcgacgat cccgctgaag gaccactcgt ggtccaacgg
     2641 caagccggtc accgcggacg acgtcgtctt caccttcgac ctcctcaagg cggccatcgc
     2701 ggagagtccg gcgaactggg cgttctacac gcccggccag ttccccgacg gcgtcaccgt
     2761 cacggcggcg gatgcccgca ccgcggtgtt ctcgttcgag aagccgtaca acccgtcgta
     2821 cctcgtctcg ctgctgcagc tgctctacat catgccctcg gcggactgga gcatcgccgc
     2881 gaccggcggc cccgtgctcg actacacgca gcccgagaac gccacggcga tctaccacta
     2941 cctgacgggc cagtccgagg accagtccac gttcgtgact aacccgctgt ggcagatcgt
     3001 gaacggcccg taccagctgt cggcgttcga gccgaccacg ggctcgttct cgctgacacc
     3061 gaacaagaag tactcgggac cgggcgactc gcgcatcgag cgggtggact tcaaggcgtt
     3121 cacctcgccc tccgcggtgt acaaccagtt cgccgccggt gagctgacca cagggcggct
     3181 ggatgccagc ttcgcctcga agatcgacga cctgcgcaag aagggctaca acgtgtacgg
     3241 cgcgccgtcc ccggcgcgct tcgacggtct cgtgatcaac ttcgacaaca aggtcgacaa
     3301 cttcgacaag atcatcgggc agctgtacgt ccgccaggcg atgcagcacc tgatcgacca
     3361 gcccggctac atcaagagcc gtggcatcca cagcggcacc gcgacggcga actacagcac
     3421 cggtggcctg aactccgcct acccgcccga gttcggcgac aaggcgacct atccctacga
     3481 cgtgaaggcg gccgagaagc tgctcaccga ccacggctgg aaggtggacg cagagaaggg
     3541 aaccacctgc gtgcggcccg gcaccggcga cgaccagtgc ggcgagggca tcccgaaggg
     3601 gcagaagatc accttcacga tcgcgtcggc caacgacccc gctttcgtcg gcgcccggga
     3661 cctggcattc gtgtcgcagc tgaagaaggt cggcatcgac gcgaaggtcg tcaccaaatc
     3721 gctgaactac atgtacgaga actacggcaa ctcctacgcg cccgacaacg cggacaagtg
     3781 ggcgatgcag gacttcggcg ccttcgtgca gacctcgtac ccgaccagca acaacgcctt
     3841 caacaccggc gggagcttca acctgggcag ctacagcaac cccgaggccg acaagctcat
     3901 cgaggcgagc accttcggcg cggacccgaa ggcgctgagc gcggaggtga ccatgctgtc
     3961 gaaggacctg ccggtgctct tcttccccac gccggacaac ctgatcgtgt ggaagaagga
     4021 cctctccggc ccgccgtcga cgttccggtc catgctgaag ttcgtctact ctccggagca
     4081 gtggtacttc accgagaagc agaagtagga cgccgtcgat gagcgcgcag atcgcgaagc
     4141 gggtcacgcc gataccgacg gtcgaccccg cacggcccgg gttcccggct cccgaggcga
     4201 tcggcgcgct catcggcgat gccagtgtgg tggcgctggg cgaggccgcg cacagcgtgg
     4261 tcgagttcac cgccctcggc gacgcgctgc tgcgcacact ggtgcaggac cacggcgtca
     4321 cggcgttcgt gatggagtcg ggcttcgccg aggggctgct gatcgacgag tggatccacg
     4381 gcgggccggg agatgtcgag gacatcgccc gcgcgggcat cacctacggg ttcggtgaat
     4441 ccgacggcat ccgccggcag ctcatctgga tgcgggagtg gaacgcggcc ggcggtgacg
     4501 tgcggttcta cggcatggac ctccccggct cgtcgacctc gccgggaacg gccgtgcgcg
     4561 cctgcctcgc gcgcgtcgcg cccg
//
LOCUS       DATMSX010000147         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_20664,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000147 DATMSX010000000
VERSION     DATMSX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20664"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="VN041_04435"
     CDS             complement(<1..>1509)
                     /locus_tag="VN041_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWU28325.1"
                     /translation="GVAPVLPLPTVPIARRVLREMVGHLIVDARPEKLGAALEKLRDT
                     GHRLNLNLLGEAVLGEAEAKRRLDGIHDLIRRDDVDYVSVKVSAVMSRISMWAFDDVV
                     EDVIERLTPLYLTAASNGTFINLDMEEYKDLDLTIAVFTRILEDPRLKGLEAGIVLQA
                     YLPDARPAMRELTAWARDRVEHGGAPVKVRLVKGANLAMEKVDAVMHGWTQAPYVSKL
                     DTDANYLRCLDEAFQPRNVAAVRVGVAGHNLFDIAFAWLLAGERGVQDSVEFEMLLGM
                     AQAQVAAVSRTVGPVLLYVPVVHPHEFDVAISYLVRRLEENASPSNFLSAAFDLAQDR
                     SLFERERLRFVDSVSRARDHSLQIGPRRAQNRTAPLHESVRPMTRPRAETQDLTGLVL
                     GIARGSSGDDDEYGADDDPFLQTAVYSRQEVAGSAAGAPGFVNTPDSDPALAVNRDWA
                     RGIFERVSAAGLSDLGIATLRASRIDDERMLAHTVTRVRDAASGWGSRPASER"
BASE COUNT          227 a          533 c          534 g          215 t
ORIGIN      
        1 gcgctcggac gccgggcggg agccccagcc ggatgccgcg tcccgaacgc gcgtgacggt
       61 gtgcgccagc atccgctcgt cgtcgatccg cgaggcgcgc agggtcgcga taccgagatc
      121 ggacaggcct gcggcggaga cgcgctcgaa gatcccgcgt gcccagtccc ggttcacggc
      181 gagcgccggg tcgctgtccg gtgtgttcac gaacccgggg gcaccggcgg cggagcccgc
      241 cacctcctga cgggagtaca ccgcggtctg caggaacggg tcgtcgtcgg ctccgtactc
      301 gtcgtcatct cccgacgagc cgcgggcgat gccgagcacc agacccgtga ggtcctgggt
      361 ctcggctctg ggcctggtca tcggacgcac cgattcgtgc agcggcgccg tacggttctg
      421 cgcccggcgc ggaccgatct gcagggagtg gtcccgggca cggctgaccg aatcgacgaa
      481 gcgcagccgc tcgcgctcga acagcgagcg atcctgcgcg aggtcgaagg cggcggagag
      541 gaagttcgag ggcgacgcgt tctcctccag ccggcgcacc aggtagctga tcgcgacgtc
      601 gaactcgtgc ggatgcacca ccggtacgta cagcagcacc ggcccgaccg tgcgcgagac
      661 cgcggcgacc tgggcctgcg ccatcccgag cagcatctcg aactccaccg agtcctgcac
      721 gccgcgctcg cccgcgagca gccaggcgaa ggcgatgtcg aacaggttgt gcccggccac
      781 gccgacgcgt accgcggcga cgttgcgcgg ctggaacgcc tcgtccaggc agcgcaggta
      841 gttggcgtcg gtgtcgagct tggagacgta gggagcctgc gtccagccgt gcatgacggc
      901 gtccaccttc tccatcgcga ggttcgcgcc cttgaccagg cgcaccttga cgggcgcccc
      961 gccgtgctcc acgcggtcgc gcgcccaggc ggtgagctcg cgcatggcgg gccgcgcgtc
     1021 gggcagatag gcctgcagca cgatgcccgc ctcgaggccc ttcagccggg ggtcctcgag
     1081 gatgcgggtg aacaccgcga tggtcaggtc gaggtccttg tactcctcca tgtcgaggtt
     1141 gatgaaggtg ccgttcgacg cggccgtcag atacaggggc gtgaggcgct cgatcacgtc
     1201 ctccaccacg tcgtcgaacg cccacatcga gatgcggctc atgaccgccg agaccttgac
     1261 cgacacgtag tcgacgtcgt cgcgacggat gaggtcgtgg atgccgtcga gacggcgctt
     1321 ggcctccgcc tcgcccagca cggcctcgcc gagcaggttg aggttcagcc gatgccccgt
     1381 gtcacgcagc ttctccagcg cggcgccgag cttctcgggg cgggcgtcca cgatcaggtg
     1441 accgaccatc tcgcgcagca cccgccgcgc gatcggcacg gtcggcagcg gcagcacagg
     1501 cgcgacccc
//
LOCUS       DATMSX010000148         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_68393,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000148 DATMSX010000000
VERSION     DATMSX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68393"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VN041_04440"
     CDS             <1..712
                     /locus_tag="VN041_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWU28326.1"
                     /translation="VAAWAEEVLAASERFRTGMETLRGEHSRELTVAASQTVAAHLLP
                     GWLVALRERQVRSGRAPTQVRMLNANSVEVETLVRSGAADLGFIESTSLPEGLATTTI
                     RLDALVVVVAPQHPWATRTDVGMDEVADASLVVREEGSGTRRTWEDAVVSRLGRMPAE
                     PVAVLSTSAAVRSAVAEGIGPAVLSGLAVADDVRLGRLVEVPLRGDAVRRPITALWRG
                     APRDLSTVSRELLEEAVG"
     gene            718..1536
                     /locus_tag="VN041_04445"
     CDS             718..1536
                     /locus_tag="VN041_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HWU28327.1"
                     /translation="MPVVAVFALLALALTGCAGGSADGDAPGAGVELPEAGAGFDYQL
                     GGAYPPPHNATVIARDRLAAPAGVGYDICYVNGFQTQPAESEKFAQRHPELVVQVDGE
                     PLADPGWPDELILDTSTEAKRTALAEIVEPWIAGCADAGYAAVEIDNLDSFSRSDGML
                     SEDDNLALAAEYARIAHAAGLAIAQKNTAESTARLVEIGYDFAVTESCAEFDECADYA
                     EAYDVVLDVEYADELGEEDFAAACADRPSGVSMILRDHDLRMASESGYLFRACP"
     gene            1595..>2116
                     /locus_tag="VN041_04450"
     CDS             1595..>2116
                     /locus_tag="VN041_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28328.1"
                     /translation="MTELRVGFLGDGHINSVLRAVVHDSRPYRVVGSFGRHSALPDAA
                     DVDVIVEAATQQAVRERVPGLLHAGVDVILLSVGALADAALRDRLLASAAGRLIVCTG
                     AIGGLDQVRALRAAGPFSEVSIESRKLPASLIQPWMPDALKADLRTRTAELVLADGPA
                     GDVAQRFPASANVA"
BASE COUNT          280 a          688 c          822 g          326 t
ORIGIN      
        1 cgtggccgcg tgggcggagg aggtgctggc cgcatcggag aggttccgca ccgggatgga
       61 gacgctgcgc ggcgagcaca gccgggagct gaccgtcgcg gccagccaga ccgtcgcggc
      121 gcacctcctg ccggggtggc tcgtggcgct gcgggagcgg caggtgcgct ccggccgggc
      181 ccccacgcag gtgcggatgc tgaacgccaa cagcgtcgag gtcgagacgc tcgtgcgctc
      241 gggggccgcg gacctgggat tcatcgagtc gacctcgctg ccggaagggc tcgcgacgac
      301 cacgatccga ctcgacgccc tggtcgtcgt cgtggcaccg cagcacccgt gggccacgag
      361 gacggacgtc gggatggatg aggttgccga cgcctccctg gtggtgcggg aagagggcag
      421 cgggacgcgg cgcacctggg aggatgcggt ggtctcccgc ctcggacgga tgccggccga
      481 accggtggcc gtgctgtcga cgtcggcggc cgtgcgatcg gccgtggccg agggcatcgg
      541 gccggccgtg ctctcggggc tcgccgtcgc cgacgacgtg cgcctagggc gactggtgga
      601 ggtgccgctg cgcggtgacg ccgtgcgtcg tccgatcacg gcactgtggc ggggagcgcc
      661 gcgtgatctg tcgaccgtga gccgggaact cctcgaagag gccgtcggat gagagggatg
      721 cctgtggtgg cggtcttcgc gctgctcgca ctcgcgctca ccggctgtgc cggcgggagt
      781 gcggacggcg acgcgccggg cgccggtgtc gagctgcccg aggcgggcgc cgggttcgac
      841 tatcaattgg gcggtgccta tccgccgccg cacaatgcga cggtgatcgc gcgtgaccga
      901 ctggccgctc ccgccggcgt gggctacgac atctgctacg tgaacgggtt ccagacccag
      961 cccgcggaat ccgagaagtt cgcgcagcgg catccggagc tggtggtcca ggtcgacggt
     1021 gagccgctcg ccgatccggg ctggccggac gagctgatcc tggacacgtc gacggaggcg
     1081 aagcgcacgg cgctcgccga gatcgtggag ccgtggatcg ccggatgcgc ggatgccggg
     1141 tacgcggcgg tcgagatcga caacctggac tccttcagcc gctccgacgg gatgctgtcg
     1201 gaggatgaca atctcgcact tgccgcggag tatgcgcgta tcgctcacgc ggcgggcctg
     1261 gcgatcgccc agaagaacac ggccgagtcg accgcgcggc tcgtggagat cgggtacgac
     1321 ttcgcagtca cggagtcgtg cgccgagttc gacgagtgcg cggactacgc cgaggcctac
     1381 gacgtggtgc tcgacgtcga gtacgccgac gagctcggtg aggaggactt cgccgcggcg
     1441 tgcgcggatc gcccgtcggg cgtgagcatg atcctgcgtg accacgacct gaggatggcg
     1501 agcgaaagcg gatacctgtt ccgcgcctgc ccttgacggc gcgtgctggt gggtgatccc
     1561 tcatccgtgc ggtcgccgtc gtagggtcga aggcatgact gagctgcggg tcgggttcct
     1621 cggcgacggg cacatcaact cggtgctgcg tgccgtcgtg catgactccc gcccgtaccg
     1681 ggtggtcggg tcgttcgggc ggcactccgc gctgccggat gctgcggacg tcgacgtgat
     1741 cgtcgaggcg gcgacccagc aggccgtgcg cgagcgtgtg cccgggttgc tccatgccgg
     1801 ggtcgacgtg atcctgctgt cggtcggtgc gctggcggat gctgcgcttc gcgaccgcct
     1861 tctggcatct gcggccggcc ggctgatcgt ctgcacgggc gcgatcggag ggctcgatca
     1921 ggtgcgtgcc ctgcgtgccg ccggtccgtt cagcgaggtg tcgatcgaga gccgcaagct
     1981 tccggcatcc ctgatccagc cgtggatgcc cgatgcgctg aaggccgacc tgcgcacccg
     2041 caccgccgag ctcgtgctgg cggacgggcc cgccggtgac gtggcgcagc gcttcccggc
     2101 atccgccaac gtcgca
//
LOCUS       DATMSX010000149         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172639,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000149 DATMSX010000000
VERSION     DATMSX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172639"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VN041_04455"
     CDS             complement(<1..527)
                     /locus_tag="VN041_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28329.1"
                     /translation="MDASVAPSRERFIDAALVVMSTRGIAASTTKLIAAQAGLSEALL
                     YKHFSDKQQLFLAVLEERLPRPRFDPNASRGEDLAEGLTALVEQLMAFFAASFPISAS
                     IFGTPELLAQHREGIAAHGHGPHEPVSVVMAYLDAERETGRVRTDADTASVARALVGA
                     AFHQGFLAAFSGADAV"
     gene            576..1199
                     /locus_tag="VN041_04460"
     CDS             576..1199
                     /locus_tag="VN041_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWU28330.1"
                     /translation="MRVVIIGASGRSGRRAVAHALAAGHEVVSVVRTLASAPQSTAVA
                     QADARDVDALAAAMVGADAVISTLGHVAKDADLTLLREGITATVKAMQRTGVRRIIAI
                     SAGAPYIAGDDPLARFIAKPILRRVLRANNEDTMAMDAVLAASDLDWTSIRPGRLIPG
                     TGRAEPRTRIDLSVRWHYSTTFDTVGRTAVDALARADWVEHAVYVTE"
     gene            1265..2989
                     /gene="poxB"
                     /locus_tag="VN041_04465"
     CDS             1265..2989
                     /gene="poxB"
                     /locus_tag="VN041_04465"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HWU28331.1"
                     /translation="MTTVAENMVRTLRANGIDRVYGIPGDSLNGFTDALRKDGTIRWV
                     HVRHEESAAFAAAADAALTGDLAVVAGSCGPGNLHLINGLYDANRSRVPVLAIAAHIP
                     TVEIGTGYFQETHPQELFRESSVYAEYVADPSQMPRLMEIAMRAAIEKRGVAVLVIPG
                     DVALAEIADDRAVVIPRANPVVTPSPAELEKAATLLNGCNRVTILAGAGVEGAHDEVI
                     ALADALAAPIVHALRGKEHIEYDNPFDVGMTGLLGFASGFRAMEDAEALLVLGSDFPY
                     TQFYPSGATTIQVDIRGEQLGKRHPLDLGLVGDVKATAEALLPRLKRKSDRAHLDDAT
                     AHYRRTRAKLDDLAVPARGKAPIHPQYLARLIDEAAASDAIFTADVGSPTVWAARYFT
                     MTEKRRLIGSFNHGSMANALMHAIGAQVARPDQQVVALAGDGGLAMMLGELITITQNK
                     LPVKTIVVNNSSLNFVELEMKAAGFVTYGTDLENPDFAAVAQALGIFARRVERSEDLL
                     DAVAEVLAHDGPALLDVVTERQELSMPPSIKAEQVKGFALYAIRTVMSGRGDELLDLA
                     KANWRQLF"
     gene            complement(3103..>3785)
                     /locus_tag="VN041_04470"
     CDS             complement(3103..>3785)
                     /locus_tag="VN041_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28332.1"
                     /translation="AVTVGMLARSLAASEEFGQAVGAFGYAADLHEQAEEPADQALML
                     TEQAKILARFGEGDDARGLLEQAAELVRPTEATGAIVEVLHNLGQAYGGAADERAFGL
                     FDEVATLAQENDAPWLLADVTDSRGRALVEFGRIDEAISALLTAADGFAGIGDAGSAS
                     GSELFAARTLVNAERLDDAVPLYRSVLDRSSEIPPLRQVGALELGDVLEKLGRAGEAA
                     QVRALIED"
BASE COUNT          586 a         1374 c         1278 g          547 t
ORIGIN      
        1 acggcatccg ccccggagaa ggcggcgagg aagccctgat ggaaggcggc gccgaccagg
       61 gcgcgggcga cggatgccgt gtcggcgtcg gtccggaccc ggcctgtctc gcgctcggcg
      121 tccaggtagg ccatcacgac cgagaccggt tcgtgcggcc catgcccgtg tgcggctatg
      181 ccctcgcgat gctgcgcgag cagctcgggg gtgccgaaga tcgatgccga gatcgggaac
      241 gatgcggcga agaacgccat cagctgctcg accagcgcgg tcagcccctc ggcgaggtcc
      301 tcgccgcgcg aggcattcgg gtcgaaccga ggccggggga gtcgctcctc gagcacggcg
      361 aggaacagct gctgcttgtc ggagaagtgc ttgtacagca gcgcctcgga caggccggcc
      421 tgcgcggcga tgagcttggt cgtcgaggcg gcgatgcccc gtgtgctcat caccaccagg
      481 gcggcgtcga tgaagcgctc acgtgagggg gcgacagagg catccatgtc acctagtgta
      541 gtgagtaagt gttcactcac cccaggagga agcgcatgcg cgtcgtcatc atcggagcat
      601 ccggcaggtc gggcagaagg gccgtcgcgc acgcgctcgc cgcaggtcac gaggtcgtct
      661 ccgtcgtgcg aacgctcgca tcggcgccgc agtcgacggc cgtggcgcag gcggatgctc
      721 gcgacgtgga tgcgctcgcc gcggcgatgg tgggagcgga tgccgtgatc tcgacgctcg
      781 gtcacgtcgc gaaggatgct gatctgaccc tcctccgcga aggcatcacc gccacggtga
      841 aagcgatgca gcgcaccggt gtccgccgga tcatcgcgat ctccgcgggc gcgccctaca
      901 tagccgggga cgacccgctc gcccgtttca tcgccaagcc gatcctgcgc cgggtactgc
      961 gggccaacaa cgaggacacc atggcgatgg atgccgtgct cgcggcatcc gacctcgact
     1021 ggaccagcat ccggcccggt cgtctgatcc cgggaacggg ccgagcggag ccccgcaccc
     1081 ggatcgacct ctcggttcgc tggcactaca gcaccacctt cgacacggtc ggacgcaccg
     1141 cggtagacgc cctcgcgcgg gccgattggg tcgagcacgc cgtgtacgtc acggaatagt
     1201 ctccgcggcg ctgaggtcga attccccggc ccggagcatg cccctcccgg cataggctcg
     1261 tgtcatgacg accgtcgctg agaacatggt ccgcacgctc cgcgccaatg gcatcgaccg
     1321 ggtctacggc atcccgggcg actcgctgaa cgggttcacg gatgccctcc gcaaggacgg
     1381 cacgatccgc tgggtgcatg tccgtcatga ggagtcggcc gcgttcgccg cagcggcgga
     1441 tgccgccctc acgggcgacc tcgcggtcgt cgcgggctcc tgtgggccgg gcaacctgca
     1501 cctgatcaac ggcctctacg acgccaaccg ctcccgcgtg ccggtgctcg cgatcgccgc
     1561 gcacatcccc acggtcgaga tcggcaccgg ctacttccag gagacccacc cgcaggagct
     1621 gttccgcgag tccagcgtct acgccgagta cgtcgccgac ccctcgcaga tgccgcggct
     1681 gatggagatc gcgatgcgcg ccgccatcga gaagcgcggc gtcgcggtgc tcgtcatccc
     1741 gggcgacgtc gccctcgccg agatcgccga cgaccgggcc gtggtcatcc cacgtgcgaa
     1801 tccggtggtc accccgagcc cggccgagct cgagaaggcc gccacgctgc tgaacggctg
     1861 caaccgggtc acgatcctcg ccggggcagg cgtcgagggc gcccacgacg aggtcatcgc
     1921 tctcgcggac gccctcgccg caccgatcgt gcacgccctg cgcggcaagg agcacatcga
     1981 gtacgacaac cccttcgacg tcggcatgac cggactgctc ggcttcgcct cgggcttccg
     2041 cgccatggag gatgccgagg ccctgctcgt cctcggatcc gacttcccgt acacgcagtt
     2101 ctatccgtca ggtgccacga ccatccaggt ggacatccgc ggcgagcagc tcggcaagcg
     2161 gcatccgctc gatctcggac tggtgggcga tgtgaaggcg acggccgagg cgctcctgcc
     2221 gcgactgaag aggaagagcg accgtgcaca cctggacgac gccacggcgc actaccgccg
     2281 cacgcgcgcg aagctcgacg acctcgccgt gcccgcgcgc ggcaaggcgc cgatccaccc
     2341 gcagtacctt gcccggctga tcgacgaggc cgcggcctcg gacgccatct tcaccgccga
     2401 cgtcgggtcg cccacggtgt gggcggcgcg ctacttcacg atgaccgaga agcgccgcct
     2461 gatcggctcg ttcaaccacg gctccatggc caacgcgctc atgcatgcga tcggcgccca
     2521 ggtcgcccgt cccgaccagc aggtggtcgc cctcgccggc gacggcggac tcgcgatgat
     2581 gctgggcgag ctcatcacca tcacccagaa caagcttccg gtgaagacca tcgtggtcaa
     2641 caactcctcg ctcaacttcg tcgagctgga gatgaaggct gccggcttcg tgacctacgg
     2701 caccgacctg gagaaccccg acttcgccgc cgtcgcgcag gcgctcggca tcttcgcgcg
     2761 gcgtgtggag cgctcggagg atctgctgga tgccgtcgcc gaggtgctcg cacatgacgg
     2821 gcccgcgctg ctcgacgtcg tcaccgagcg gcaggagctg tcgatgccgc cgtcgatcaa
     2881 ggccgagcag gtgaagggct tcgcgctcta cgccatccgc acggtcatgt cgggccgcgg
     2941 cgacgagctg ctcgatctgg cgaaggccaa ctggcgccag ctgttctgag cctgctgctc
     3001 aagccaccac gcagacaccg agccaccacg cagatcacgg tgatctgcgt ggtggctcgg
     3061 cgtttcaatg gtggttcggc gagctgcgtg ccggaaccgc ggtcagtcct cgatcagcgc
     3121 ccgcacctgg gcggcctcgc cggcgcggcc gagcttctcg agcacgtcgc ccagctccag
     3181 cgcacccacc tggcgcagcg gcgggatctc cgaggaccgg tccagcacgc tgcggtacag
     3241 cggcaccgcg tcgtccagcc gctccgcgtt cacgagcgtg cgcgccgcga acagctcgga
     3301 gccactggcg gatcccgcat ccccgatgcc ggcgaacccg tccgccgcgg tcagcagcgc
     3361 cgagatcgcc tcgtcgatgc gcccgaactc caccagggcc cgcccgcgcg agtcggtcac
     3421 gtcggcgagc agccaggggg cgtcgttctc ctgggcgagc gtcgcgacct cgtcgaacag
     3481 cccgaacgcg cgctcgtcgg ccgcgccgcc gtacgcctga cccaggttgt gcagcacctc
     3541 gacgatggct ccggtggcct cggtcggtcg cacgagctcg gccgcctgct ccagcagtcc
     3601 gcgggcgtcg tcgccctcgc cgaaacgggc gaggatcttc gcctgctcgg tgagcatcag
     3661 cgcctggtcg gcgggctcct cggcctgctc gtgcaggtcg gccgcgtatc cgaaggcgcc
     3721 gacggcctgc ccgaactcct cggatgccgc cagcgaccgg gccagcatcc cgaccgtgac
     3781 ggccc
//
LOCUS       DATMSX010000150         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_11476,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000150 DATMSX010000000
VERSION     DATMSX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11476"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN041_04475"
     CDS             complement(<1..207)
                     /locus_tag="VN041_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28333.1"
                     /translation="MHDGQLAVDEEIAAELIARDLPSFAGPAVVAVPGAGTVNAIFRV
                     GDEVAARFPLAPASAQDLSREASGL"
     gene            complement(233..1651)
                     /locus_tag="VN041_04480"
     CDS             complement(233..1651)
                     /locus_tag="VN041_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HWU28334.1"
                     /translation="MTSAENDTVVETPTDAPKDANSSRKQPFPQGFLDTISTGWADRP
                     ESLPAARAQAPYAAARRDAVSKAFPGKRLVVPAGSLKQRSNDTDYPFRAHSAFLHLTG
                     WENEAEPDSVLVFEPTDAGHEVTLYFRERADRTTSEFYSDATIGEFWIGPRPSLAGVA
                     ADLAVATAHIDAFSAGDDDLVVGADDELTRFVSELRLVKDEFEIAEMRRAVAVTASGF
                     EDVIRDFDRAVAHPRGERIVEGVFHQRARSDGNWEGYDTIAASGPHACYLHWTRNDGT
                     VVPGDLILIDAGVEVDSQYTADITRTLPVNGTFSDVQRRVYEVVLEAADAAFAAAKPG
                     VRFRSVHEAAMKVIAERTAEWGLLPVTAEEALDADRGGQQRRYMVHGTSHHLGIDVHD
                     CAQARREMYYDGVLEAGMVFTIEPGLYFQIDDLTVPEELRGIGVRIEDDILMTEDGPV
                     NLSADIPRTVADVEAWIARLKR"
     gene            complement(1727..2752)
                     /locus_tag="VN041_04485"
     CDS             complement(1727..2752)
                     /locus_tag="VN041_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWU28335.1"
                     /translation="MLRLVGILITVLFAIATAVLVWPQFFHLEDTFPIAQIIASRALV
                     IGAFLVVAVLALLLLIARPLRGFAASILVVALIGAGATGLVGGLRGVGTGTLPAKTDA
                     ALRVLTWNTNGAAVSAEQIAKVIEEQQVDVVALPETSEKVGERIALMMRQAGNPMWVH
                     NVNIRPDVSNGPQAWQTTILISAKLGNYSVIESSRDGSSNTGSVPSVVAMPVNGKGPT
                     IVAVHAVAPREKAMSRWKSDLAWIADQCPAGDFILAGDFNSTLDHMASFGDDGGTMGR
                     CRDVASSTGNGMLGTWPASVPALLGAPIDHIMASENWRATGSEVLEDAGGSDHRALVA
                     QLEPVSP"
     gene            2808..3683
                     /locus_tag="VN041_04490"
     CDS             2808..3683
                     /locus_tag="VN041_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWU28336.1"
                     /translation="MTQGAHSTVSGPSDLHLHSNHSDGTESPADVVRAAHAHGIRTVA
                     LTDHDRTTGWQEAGDVAVALGMTFIPGMEFSSRHEWRSVHVLGYLFDPTDPALVAETE
                     RIREDRVGRAERIVRSIARDYELSWDDVLLQTTPDATVGRPHIADALVARGIVRDRTE
                     AFDGILHPRAGYYEPHYAPDPLTVVRLITRAGGVPVIAHPATSGRDRMMPVSFFDRLV
                     AEGLGGLEIDHRENTEDGKRALREIAARHDLIITGSSDYHGAGKPNLPGENTTSDEMV
                     ARIIARGTGTPPRRP"
BASE COUNT          576 a         1323 c         1323 g          550 t
ORIGIN      
        1 gagaccggat gcctcgcggg acagatcctg cgccgaggcc ggcgccagcg ggaagcgtgc
       61 ggcgacctcg tcgccgacgc ggaagatcgc gttcaccgtc ccggcgccag gaacggccac
      121 gacggccggc ccggcgaacg acgggagatc acgggcgatc agctcggccg ctatctcctc
      181 gtcgacggcc agctgtccgt cgtgcatccg tcccgtcacg ctgagggtcg gttcagcgct
      241 tcagccgcgc gatccacgcc tcgacgtcgg cgacggtgcg cgggatgtcg gcggagaggt
      301 tgacggggcc gtcctcggtc atcaggatgt cgtcctcgat gcgcacgccg atgccgcgca
      361 gttcctcggg aaccgtgaga tcgtcgatct ggaagtacag tcccggctcg atcgtgaaga
      421 ccatgccggc ctcgaggacg ccgtcgtagt acatctcgcg acgcgcctgc gcgcagtcgt
      481 gcacatcgat accgaggtgg tgcgaggtgc cgtgcaccat gtaccggcgc tgctggccgc
      541 ccctgtcggc gtccagcgcc tcctcggcgg tcacgggcag caggccccac tcggccgtgc
      601 gctcggcgat gaccttcatc gccgcctcgt gcacgctgcg gaagcgcaca cccggcttcg
      661 cggcggcgaa ggcggcgtcc gcagcctcca gcacgacctc gtagacgcgg cgctgcacgt
      721 cggagaacgt gccgttcacg ggcagtgtgc gggtgatgtc ggcggtgtac tggctgtcga
      781 cctcgacgcc cgcgtcgatc aggatcagat caccggggac gacggtgccg tcgttgcgcg
      841 tccagtgcag atagcaggcg tgcgggccgg aggccgcgat cgtgtcgtag ccctcccagt
      901 tgccgtcgct gcgggcgcgc tggtggaaga cgccctcgac gatgcgctcg ccgcgcgggt
      961 gcgcgacggc gcggtcgaag tcgcggatga cgtcctcgaa cccggatgcc gtgacggcca
     1021 ccgcgcgacg catctcggcg atctcgaact cgtccttcac caggcgcagc tcggagacga
     1081 agcgtgtcag ctcgtcatcc gcgcccacga ccaggtcgtc gtcgccggcg ctgaaggcgt
     1141 cgatgtgcgc ggtggccacg gccaggtcgg cggcgacgcc ggcgagcgag ggccgcggac
     1201 cgatccagaa ctcgccgatc gtggcatccg agtagaactc gctggtggtg cggtcagcgc
     1261 gctcgcggaa gtacagcgtc acctcgtgtc cggcgtccgt cggctcgaac accagcacgg
     1321 agtcgggctc ggcctcgttc tcccagccgg tcaggtgcag gaacgccgag tgcgcgcgga
     1381 acgggtagtc ggtgtcgttg ctgcgctgct tgagcgagcc ggccgggacg accaggcgct
     1441 tgccggggaa ggccttcgac acggcgtcgc ggcgggcggc tgcgtaaggt gcctgggcgc
     1501 gcgcagcggg cagcgactcc gggcggtccg cccacccggt cgagatggtg tcgaggaagc
     1561 cctgcgggaa gggctgcttg cgggagctgt tggcatcctt gggagcgtcg gtcggggtct
     1621 cgacgacggt gtcgttctca gcgctggtca tgtgctccat tgtctcatcg acgcaggaag
     1681 cccgcttcgt gctcaggcgc cgccggagat cacgcgcgtt ccgaggtcac ggcgagaccg
     1741 gctccagctg ggcgaccagc gcgcggtggt cgctgccgcc ggcatcctcg agcacctcgg
     1801 atccggtggc tcgccagttc tcgctcgcca tgatgtggtc gatgggcgca ccgagcagcg
     1861 ccggcacgct cgcgggccag gtgcccagca ttccgttccc ggtgctgctg gcgacatcgc
     1921 ggcagcgccc catggttccg ccgtcgtcac cgaaggacgc catgtggtcg agggtcgagt
     1981 tgaaatcgcc cgcgaggatg aaatccccgg cggggcactg atcggcgatc cacgccagat
     2041 ccgacttcca gcgactcatg gccttctcgc gcggggcgac cgcgtgcacg gccacgatcg
     2101 tcgggccctt gccgttgacg ggcatcgcca cgacgctcgg caccgagccg gtgttgctgg
     2161 acccgtcacg ggaggactcg atgaccgagt agttcccgag cttcgcggag atgaggatcg
     2221 tggtctgcca cgcctgcggc ccattggaca cgtcggggcg gatgttcacg ttgtgcaccc
     2281 acatcgggtt ccccgcctga cgcatcatca gagcgatgcg ctcgccgacc ttctccgagg
     2341 tctccggcag cgccacgacg tcgacctgct gctcctcgat gaccttcgcg atctgctccg
     2401 cggacacggc cgcgccgttg gtgttccagg tgagcacgcg cagcgcggca tccgtcttcg
     2461 ccggcagcgt gccggtgccg acgccgcgca gacccccgac cagcccggtc gcccccgcgc
     2521 cgatcagcgc gacgacgagg atcgacgccg cgaagccccg cagcggcctc gcgatgagca
     2581 gaaggagcgc aagcaccgcg acgaccagga acgcaccgat caccagagcg cgggaggcga
     2641 tgatctgggc gatcgggaac gtgtcctcca ggtggaagaa ctgcggccac acgagcaccg
     2701 ccgtcgcgat cgcgaacagc acggtgatga ggattccgac cagacgtagc atcgtccgaa
     2761 agcctaggcg aatcttgctg tgacacccct ggcggtacgc tcggaggatg acccagggcg
     2821 cccactccac cgtctccggt ccgagtgacc tgcacctgca ttcgaaccac tccgacggca
     2881 cggaatcccc ggccgacgtc gtgcgtgcgg ctcacgccca cggcatccgc accgtcgccc
     2941 tgaccgacca cgatcgcacc acgggctggc aggaggcggg cgacgtcgcg gtcgcgctgg
     3001 ggatgacgtt catccccggc atggagttct cctcccggca cgaatggcgc agcgtgcacg
     3061 tgctgggcta cctgttcgat ccgaccgatc cggcgctggt cgccgagacc gagcggatcc
     3121 gcgaggaccg ggtcgggcgc gccgagcgga tcgtccgcag catcgcccgc gactacgagc
     3181 tgagctggga cgatgtgctg ctgcagacca caccggatgc caccgtcggc cgcccgcaca
     3241 tcgccgacgc cctggtggcg cgcgggatcg tccgcgatcg cacggaggcg ttcgacggca
     3301 tcctgcatcc gcgtgccggt tactacgaac cgcactacgc gccggacccg ctcaccgtgg
     3361 tgcggctgat cacacgcgcc gggggagtgc cggtgatcgc gcacccggcg acctccggcc
     3421 gggaccggat gatgccggtc tccttcttcg accggctcgt cgcggaagga ctcggcggac
     3481 tcgagatcga ccaccgcgag aacaccgagg acggcaagcg ggcgctgcgc gagatcgcgg
     3541 cgcgacacga cctcatcatc acgggctcca gcgactacca cggcgcgggc aagccgaacc
     3601 tgcccggcga gaacacgacc tccgacgaga tggtcgcccg gatcatcgcc cgcggcacgg
     3661 gcacaccgcc gcggcgcccg tgaccaggac gcacgtcagc ccgctgcacc gtggaagcag
     3721 aacagccgcg agccccggat cgggacgcgc ggctgctgcg tgagtgatcg tc
//
LOCUS       DATMSX010000151         3956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_182259,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000151 DATMSX010000000
VERSION     DATMSX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182259"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VN041_04495"
     CDS             complement(<1..180)
                     /locus_tag="VN041_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HWU28337.1"
                     /translation="MTTTAALLAAARERLGDIPREGLGLLRASRWRRDRIVRVGDAWH
                     LGVLLLTDDGVLSTGE"
     gene            complement(197..832)
                     /locus_tag="VN041_04500"
     CDS             complement(197..832)
                     /locus_tag="VN041_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWU28338.1"
                     /translation="MDDQTSQHESRLTALTISCTLKPSPAPSSSEKMSGQISRLLEEH
                     GVATSHVRAVDHDILPGVDKDMGEGDEWPSLREKVLAADIVVLVTPTWLGQQSSVAQR
                     VLERLDAELGETDAEGRPTMFDKVALAGVVGNEDGAHHIVAVLYQSLGDVGFSVPAQG
                     CVYWNGEAMQKTDYKDLPETPASVESSMSTAVANAVHLARLLKREGYPSAG"
     gene            1078..1347
                     /locus_tag="VN041_04505"
     CDS             1078..1347
                     /locus_tag="VN041_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWU28339.1"
                     /translation="MPRGRGSNSLKDPDLYEELRDDGASKEKAARISNAAARDGRKSV
                     GKRGGKAEDYEDRTVDELKSRAKELGLKGYSGKRKSELISMLRDH"
     gene            1395..1937
                     /locus_tag="VN041_04510"
     CDS             1395..1937
                     /locus_tag="VN041_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HWU28340.1"
                     /translation="MTPTKKPSSTQAAKKQNTTPLTKDQNAESGFHASEKLGQSLQRV
                     LVDLVDLQIQGKQAHWNVVGKNFRDTHLQLDEVVEAARRLGDTVAERMRALHAIPDGR
                     SRTVAETTSLPPYPDGEVSTTETVDLVTERLEAAIATVRDVHDAVDEEDPTSADILHV
                     VLEELEQFAWMVSAENRTPA"
     gene            2110..2973
                     /gene="purU"
                     /locus_tag="VN041_04515"
     CDS             2110..2973
                     /gene="purU"
                     /locus_tag="VN041_04515"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWU28341.1"
                     /translation="MPQSDTARLLIACDDQPGIVAAVAGVLASHGANIISLDQHSTDA
                     HGGRFFQRTVLHLEGLAANRPALEASIAEVAERFGMEWSLHDTSHRKRVAIFVSKYDH
                     CLMELLWRTQRGELDIDVTMVISNHPDLAESVRSFGVPFVHIPRGDGPDAKAEMEERQ
                     LELLQGNVDLVVLARYMQILTDDFIGRIGAPVINIHHSFLPAFIGANPYARAKERGVK
                     LIGATAHYATADLDEGPIIEQDVTRVTHAESAAELQRRGADVERYVLARAVKWHAEDR
                     VIVHGRSTVIL"
     gene            3014..3586
                     /locus_tag="VN041_04520"
     CDS             3014..3586
                     /locus_tag="VN041_04520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWU28342.1"
                     /translation="MPKLMIVVGSVREGRIGLPIAQWVRGAAERHGGFEIDWADLKEI
                     SLPLMTEPNHPRLRQYTQASTIAWSERVDAADALIFVQPEYNFSYSPALKNAMDHLSQ
                     EWWRKPLGTVSYGGISGGTRGTAALRVPEMALGLVPTTVNVEIPWAFKQIEEGVFTPL
                     DSQATMLERMLNELTSLDAALAPLRAAPRP"
     gene            complement(3765..>3956)
                     /locus_tag="VN041_04525"
     CDS             complement(3765..>3956)
                     /locus_tag="VN041_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitropropane dioxygenase"
                     /protein_id="HWU28343.1"
                     /translation="EIEASGAIAPFPAQNWLTGRFRAVAGQRDLGDLQSLWLGQSAPL
                     ARWDAASEVFAELLAGVPA"
BASE COUNT          669 a         1381 c         1347 g          559 t
ORIGIN      
        1 ctcgccggtg gagagcactc cgtcgtcggt gagcagcagc acgcccagat gccaggcatc
       61 cccgacccgc acgatccggt ctcgccgcca gcgcgaagcg cgcagcaggc cgagcccctc
      121 gcgcgggatg tcgcccagcc gctcccgcgc agcggcgagg agtgcagcgg tcgtggtcac
      181 ccgttcaggc tagtcgtcac ccggcggagg ggtatccctc ccgcttcagc agtcgggcca
      241 gatgcaccgc gttggcgacg gccgtgctca tggacgactc cacagaggcg ggcgtctcgg
      301 gcagatcctt gtagtcggtc ttctgcatcg cctcaccgtt ccagtacacg catccctgcg
      361 caggcacgct gaagcccaca tcgcccagcg actggtagag cacagccacg atgtgatgcg
      421 cgccgtcctc gttgcccacc acgccggcca gcgcgacctt gtcgaacatc gtgggacgcc
      481 cctcggcgtc ggtctccccg agctcggcgt ccaggcgctc gagcacgcgc tgcgcgacgc
      541 tggactgctg gccgagccac gtcggggtca cgagcacgac gatgtcggcg gcgagcacct
      601 tctcgcgaag cgacggccac tcgtccccct cacccatgtc cttgtccacg ccgggcagga
      661 tgtcgtggtc gaccgcccgc acatgcgatg tcgcgacacc atgctcctcg agcagtctgc
      721 tgatctgccc gctcatcttc tccgagctcg acggcgcagg cgacggcttg agcgtgcagg
      781 agatggtcag tgcggtcagc cggctctcgt gctgtgacgt ctggtcgtcc atgatggttc
      841 ctctcgatcg gtcggggcct gcccgtgcgt gcgagctgca gagcgacact ggcacccgac
      901 gagcgcggtg tcccccgcgt tgacgcgccc ctccggcggt gctaacgggc cgcgcctcgc
      961 gttgttgtca tgagccactc gggtgcacgc cggcgcgcag cactgtcaac cccctgtcgg
     1021 cgacggcggg cgcctcggca tcctggcggc acagacacga cggagaagga gagcatcatg
     1081 ccccgtggac gcggttcgaa cagcctgaag gatccggatc tctacgaaga gctccgtgac
     1141 gacggagcgt cgaaggagaa ggccgcgcgg atctcgaacg cggctgcccg tgacgggcgg
     1201 aagtccgtcg gcaaacgcgg cggcaaggct gaggactacg aggatcgcac ggtcgacgaa
     1261 ctcaagtccc gcgcgaagga gctcggcctc aagggctact ccggcaagcg caagtcggag
     1321 ctgatctcga tgctgcgcga tcactgacca cgatcaccga tgaccccacc cagacgaacc
     1381 ggaggagaac gaagatgacg cccacgaaga agccgagctc gacgcaggcc gcgaagaagc
     1441 agaacacgac gccgctcacg aaggaccaga acgccgagag cggattccac gcatccgaga
     1501 agctgggcca gagcctgcag cgggtgctgg tcgacctggt ggacctgcag atccagggga
     1561 agcaggcgca ctggaacgtc gtcgggaaga acttccgtga cacgcacctg cagttggacg
     1621 aggtggtcga ggcggcgcgg cggctgggag acacggtcgc cgagcggatg cgggcgctgc
     1681 acgcgatccc cgacggacgc agccgcacgg tggcggagac cacgtcgctg ccgccgtatc
     1741 cggacgggga ggtcagcacg accgagaccg tcgacctggt caccgagcgg ctggaggccg
     1801 cgatcgccac ggtgcgcgac gtgcacgacg cggtcgacga ggaggatccc accagcgccg
     1861 acatcctgca cgtcgtcctc gaggagctcg agcagttcgc ctggatggtc agcgccgaga
     1921 accgcacccc ggcgtgatcc actctcgcgg cggtggtcct cccgccgccg cgaggccgcg
     1981 gctccggccg tcatggaccc gcacggccgg tgcatgacgg ccgggattcg ccgggccggc
     2041 gcgagaaggg cagaatcgag gcacacctcc gcgtctctcc gcccgaggga tctcagctca
     2101 ggagcatcca tgcctcagtc cgacaccgcc cgcctcctca tcgcctgcga cgaccagccc
     2161 ggcatcgtcg ccgccgtcgc cggagtgctg gcgtcgcacg gggccaacat catctcgctc
     2221 gaccagcact ccacggacgc gcacggcggc cgcttcttcc agcgcacggt gctgcacctg
     2281 gagggcctcg cggcgaaccg ccctgcgctc gaggcgtcga tcgccgaggt cgcggagcgc
     2341 ttcggcatgg agtggtcgct gcacgacacc agccaccgca agcgcgtcgc gatcttcgtg
     2401 tcgaagtacg accactgcct gatggagctg ctgtggcgca cgcagcgcgg tgaactcgac
     2461 atcgacgtga cgatggtcat ctcgaaccat cccgacctcg cggagtcggt gcgctcgttc
     2521 ggcgtgccgt tcgtgcacat cccgcgcggc gacggacccg atgccaaggc cgagatggag
     2581 gagcgccagc tggagctgct gcagggcaac gtcgacctgg tcgtgctggc gcgctacatg
     2641 cagatcctca ccgacgattt catcgggcga atcggcgccc ccgtcatcaa catccaccac
     2701 tccttcctgc cggcgttcat cggcgcgaac ccgtatgcgc gcgccaagga gcgcggtgtc
     2761 aagctcatcg gcgcgaccgc ccattacgcg acggccgacc tcgacgaggg cccgatcatc
     2821 gagcaggacg tcacccgcgt cacgcatgcc gaatcggccg ccgagctgca gcgccgcggc
     2881 gccgacgtgg agcgctacgt gctcgcacgc gccgtgaagt ggcacgccga ggaccgcgtg
     2941 atcgtgcacg gccgttccac ggtcatactc tgagacacgg ccgcactctg agacggagag
     3001 ccgggaggtc cacatgccga agctgatgat cgtcgtcggg agcgtgcgag agggccgcat
     3061 cggcctgccg atcgcgcagt gggtgcgcgg agccgccgaa cggcacggcg ggttcgagat
     3121 cgactgggcg gatctgaagg agatcagcct gcctctcatg accgagccga atcatccgcg
     3181 gctgcggcag tacacgcagg cgtcgacgat cgcatggagc gagcgcgtgg atgccgcgga
     3241 tgcgctgatc ttcgtgcagc cggagtacaa cttcagctac tcccccgccc tgaagaacgc
     3301 gatggaccac ctctcgcagg agtggtggcg caagccgctg ggcaccgtga gctatggcgg
     3361 gatctccggc ggcacgcgtg gtacggctgc gctgagggtt cccgagatgg ccctcggtct
     3421 cgtgccgacc acggtgaacg tcgagatccc gtgggcgttc aagcagatcg aggagggtgt
     3481 gttcacgcca ctcgactcgc aggcgacgat gctggagcgg atgctgaacg agctgacatc
     3541 gctggacgcc gcgctcgcgc cactgcgcgc ggccccgcgc ccctgacccg cgcttcctcg
     3601 ctcgcgatcg cggcgtcggg tcgcgattcg tcccggaatg ccgctcgatc gcgcgtccag
     3661 gtcgcagttg atgccggtat ccggccgcca tggcggcacc aaatgcgact tgggcgacga
     3721 atcatcgcgc agagcggcac caaatgcgac ctgggcgacg agcgtcaggc ggggacgccc
     3781 gccagcagct cggcgaagac ctcggacgcc gcgtcccagc gtgccagcgg agccgactgc
     3841 ccgagccaca gcgactgcag gtcgcccaga tcgcgctggc cggcgaccgc acggaagcgc
     3901 ccggtcagcc agttctgcgc cgggaacggt gcgatggcgc cggatgcctc gatctc
//
LOCUS       DATMSX010000152         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_106448,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000152 DATMSX010000000
VERSION     DATMSX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106448"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VN041_04530"
     CDS             <1..618
                     /locus_tag="VN041_04530"
                     /inference="COORDINATES: protein motif:HMM:NF042854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6805 domain-containing protein"
                     /protein_id="HWU28344.1"
                     /translation="LDGLFADDSRMGHIAHGPLLPFADLPVLDPDEVPEGLEPFAGIH
                     DSRYTVYWPISDGDPAARTARLRELDGGLAVDEATVDAVALGEQQPESDHAFSGTGAA
                     VLTTGDQGWRTTSESMAVRMRTAHARVLRVRYRLQQTPTAVVVRIDDALIAEERFDAA
                     TGVDAEVLDIDYPIGELAGRASARVGFTAVDGLPTPAIGTVRSLR"
     gene            647..1612
                     /locus_tag="VN041_04535"
     CDS             647..1612
                     /locus_tag="VN041_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HWU28345.1"
                     /translation="MTQELDDFWAHDPFILPFPAERQYYLYTASRTEPVVLAYRSSDL
                     TSWSDDPVEVFRVPEGTWASPAEAPWAPEVHEHQGRYYLFTTLHDSSRALEIPGGPRF
                     RAVSDLTGADIVPTARGTVIAVADHPAGPFELLRTDAPVAPAEFMTLDGTLFVDDDGA
                     PWMVYAHEWVQIFDGTIEAVPLTDDLRAAAGDPILLFRGSEARLLTQLPPSARTRMPY
                     VTDGPQLRRLPGGGLLMIWATYRPLGDEGSEYVETMAISREGDLTGPWEQLDVLVDGN
                     AGHGMLFETFEGRLMLVLHRGMNTPRVRAELHEVVEDGDGLRLLG"
     gene            complement(1632..2858)
                     /locus_tag="VN041_04540"
     CDS             complement(1632..2858)
                     /locus_tag="VN041_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWU28346.1"
                     /translation="MPRRTSRDIRSDSMIDVLHAVLSAGETTRNDIARSTGLSAATVV
                     TIVSELIAEGVLAETKVAAGRVGRPTSVFGMNAARGYVVGIDVAETYIRVIVFDTALN
                     QMAYAESQHDEHVLEPDTIVEAIGAELDGVLEREGIAREDVIGVGISLPGLVHEPTGV
                     AVVVPHWQWHSVELERLRERIGLPLVIENPLRAMVNAELWLGRGRSASSLATINLGTG
                     VGAGIVLEGHVLRGATNSAGEWGHSLLMLDGRLCRCGRRGCVEAYIGVPGIHQTLREL
                     APGHPLAEVELQSDFITRLAEAADGADPDAAAQETIAKTAYYLGSALADLVAVVNPEV
                     VTLTGWTTWALGDRLLPIARERMRSQAPGRAGAAVDLEVSSVRSNPVATGIAMIALER
                     SMVDAGLLTTRIPVAL"
     gene            complement(2867..>3440)
                     /locus_tag="VN041_04545"
     CDS             complement(2867..>3440)
                     /locus_tag="VN041_04545"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28347.1"
                     /translation="WRHPVSRHWQDADAGTIEVTCFAAGGEPRLTRDGADVPLSFDDD
                     GGFWTAVIPASDAVLVLEVRDGDDVVARDELRRAGEPARLDVSIWRAPADAVRRCRAA
                     GVAIDGVAQIECELVDVDGQPAKGDLPVTVESSGRILGLENGDLADVTSYDGLQRSTF
                     DGRLAVFARSGEGAAVRLSAPGVDAVTVQI"
BASE COUNT          501 a         1270 c         1188 g          481 t
ORIGIN      
        1 ctggacggcc tgttcgccga cgacagccgg atggggcaca tcgcgcacgg cccgctgctg
       61 cccttcgccg acctgccggt gctcgatccc gacgaggtgc cggagggtct cgagcccttc
      121 gccggcatcc acgactcccg gtacacggtg tactggccga tctccgacgg cgaccccgcg
      181 gcgcgcaccg cgcggcttcg cgaactcgac ggcggcctcg ccgtggacga ggcgaccgtc
      241 gatgcggtgg cgctgggcga gcagcagccg gaatccgatc acgcgttcag cggcacgggc
      301 gcggcggtgc tgacgaccgg cgaccagggg tggcggacca cctccgagag catggcggtg
      361 cggatgcgga cggcgcacgc ccgcgtgctt cgcgtgcggt accggctgca gcagacaccc
      421 accgccgtgg tcgtgcgcat cgacgacgcg ctcatcgccg aggagcgctt cgacgcggcc
      481 accggggtcg acgccgaggt gctcgacatc gactacccga tcggcgagct cgcgggccgc
      541 gcttcggcac gggtggggtt caccgcagtg gacggactgc ccacacctgc gatcggaacg
      601 gtcaggtcgc tgcgctgagg cagcacggac gagaaggagc atgagcatga ctcaggaact
      661 ggacgacttc tgggcgcacg acccgttcat cctgccgttc ccggcagaac ggcagtacta
      721 cctgtacacc gcctcgcgga cggagccggt ggtgctggcc taccgcagct ccgacttgac
      781 gagctggtcg gatgatcccg tcgaggtgtt ccgcgtcccg gagggaacct gggcgtcgcc
      841 cgccgaggcg ccgtgggcgc ccgaggtgca cgagcatcag ggccggtact acctgttcac
      901 gacgctgcac gactcgtccc gcgcgctcga gatccccggt ggaccgagat tccgcgccgt
      961 atccgacctg acaggggccg acatcgtgcc cacggcccgc ggcacggtca tcgcggtcgc
     1021 cgatcatccc gccggaccct tcgagctgct gcgcacggat gcccccgtcg ctcccgcgga
     1081 gttcatgacg ctcgacggca cgctgttcgt ggacgacgac ggcgccccgt ggatggtcta
     1141 cgcccacgag tgggtgcaga tcttcgacgg cacgatcgag gcggtccccc tcacggacga
     1201 tctccgcgcg gcagccggcg atccgatcct gctgttccgc ggttccgagg cgcgactgct
     1261 cacgcagctg ccgcccagcg ccaggacgcg catgccgtac gtcaccgacg gcccgcagct
     1321 gcgcaggctc ccgggtggcg gactgctcat gatctgggcg acgtaccgtc ccctcggcga
     1381 cgagggatcg gagtacgtcg agacgatggc gatctcgcgc gaaggagacc tgaccggccc
     1441 gtgggagcag ctcgacgtgc tcgtcgacgg gaacgccgga cacgggatgc tcttcgagac
     1501 gttcgagggg cgcctgatgc tggtgctgca ccgcggcatg aacacgcctc gcgtgcgtgc
     1561 cgagctgcac gaggtcgtcg aggacggcga cggactccgg ctcctcggct gacggatcag
     1621 agcctgacgg gtcagagggc gacggggatc cgggtggtga gcagcccggc atccaccatc
     1681 gagcgctcca gcgcgatcat ggcgatgccg gtcgcgacgg ggttgctgcg cacggatgag
     1741 acctcgagat cgacggcggc acccgcccgc ccgggcgcct gggaccgcat ccgctcccgc
     1801 gcgatcggaa gcagccggtc gccgagggcc caggtcgtcc agcccgtgag cgtgacgacc
     1861 tcggggttga cgaccgcgac cagatccgcg agcgccgaac cgaggtaata ggccgtcttg
     1921 gcgatcgtct cctgcgccgc ggcgtcggga tcggcgccgt cggccgcctc ggccagccgc
     1981 gtgatgaagt cgctctgcag ctcgacctcg gccagcggat gcccgggggc cagctcccgc
     2041 agggtctgat ggatgccggg gacgccgatg taggcctcga cgcagccgcg ccggccgcag
     2101 cggcacagcc tgccgtcgag catgagcagc gagtgccccc actcgccggc tgagttcgtc
     2161 gcgcctctca gcacatgccc ttccagcacg attcccgcgc cgacgccggt gccgaggttg
     2221 atcgtggcca ggctcgacgc cgatcggccg cggccgagcc acagctcggc gttgaccatc
     2281 gccctgagcg ggttctcgat cacgaggggc aggccgatgc gctcgcgcag acgctccagc
     2341 tccacgctgt gccactgcca gtgcggcacg acgacggcga cgccggtggg ctcgtgcacc
     2401 aggcccggca gcgagatgcc caccccgatc acgtcctcgc gcgcgatgcc ctcgcgctcc
     2461 agcacgccgt ccagctccgc gccgatggcc tcgacgatcg tgtcgggctc cagcacgtgc
     2521 tcgtcatgct ggctctcggc gtaggccatc tggttcagcg ccgtgtcgaa cacgatcacg
     2581 cggatgtagg tctcggcgac gtcgatgccg accacgtacc cgcgggccgc gttcatgccg
     2641 aacaccgacg tcgggcgtcc cacgcgtccg gccgcgacct tcgtctcggc gaggaccccc
     2701 tcggcgatca gctcgctcac gatcgtgacg accgtggcgg cactgagtcc cgtggagcgg
     2761 gcgatgtcgt tgcgcgtggt ctcgcccgcc gagagcacgg cgtgcagcac gtcgatcatc
     2821 gagtcgctgc ggatgtcgcg cgacgtccgc ctcggcacga cacctctcat atctgcaccg
     2881 taacagcatc gacccccggc gcgctcagac gcacggcggc gccctccccg ctgcgcgcga
     2941 acaccgcgag ccggccgtcg aacgtgctcc gctgcaggcc gtcgtacgac gtgacgtcgg
     3001 ccaggtcccc gttctccagg ccgaggatcc tgccgctcga ctccacggtc accggcaggt
     3061 cgcccttggc cggctgaccg tccacgtcga ccagctcgca ctcgatctgg gcgacgccgt
     3121 cgatcgcgac acccgcggcg cgacagcgcc gcacggcatc cgccggtgcc cgccagatcg
     3181 agacgtcgag acgcgccggc tcccccgcac gacgcagctc gtcgcgcgcg accacgtcgt
     3241 cgccgtcgcg cacctccagc acgaggacgg cgtcggatgc cgggatcacc gcggtccaga
     3301 atccgccgtc gtcgtcgaag gacagcggca cgtccgcccc gtcacgcgtg agccgcggtt
     3361 cgccgcccgc ggcgaagcag gtcacctcga tcgtcccggc atccgcgtcc tgccagtgcc
     3421 gcgagacggg atgccgccag
//
LOCUS       DATMSX010000153        35050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_134501,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000153 DATMSX010000000
VERSION     DATMSX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 35050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35050
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134501"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VN041_04550"
     CDS             <1..278
                     /locus_tag="VN041_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28348.1"
                     /translation="RAELLGLPLAGTMISLMLAGCAAVMLVVTGAAVSVGLPNLDAGA
                     AWLTGGSLLLAALVSGMLASRREVRLRMLVRDALMPGDVSGDDDVDA"
     gene            340..1008
                     /locus_tag="VN041_04555"
     CDS             340..1008
                     /locus_tag="VN041_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWU28349.1"
                     /translation="MRLISYNLRKHRAASELVALVGRHDPDILCLQECDTTALPESID
                     DLSLVRATSGNRLGLAMYLRSNQFRVERVRTIGLKKSLHDRVLKPAEERVLGALLTNI
                     DTGRQLIVASFHAAPLTALNSLRRNQIRAGLDALQALAPGKPVLMVGDYNYPVFKENL
                     GQTVREHGYRLTLSDDRTYTRYRVFRGHYDFATSNGFDIADITTLPQGTSDHRPILIT
                     AEAA"
     gene            1153..3315
                     /locus_tag="VN041_04560"
     CDS             1153..3315
                     /locus_tag="VN041_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWU28350.1"
                     /translation="MVAVIASTVLVAGLLVVAGGASASAATATEVESNDEVSTANPLP
                     LGTTMHGTAYSSSDKDQDYYVFDVPESGQLHVDLQFPAGLQGLYAYEVEIQGARGDAE
                     TYFRWVPTPDWSDGTRLRSQAPFLQAGRYWLLIAAAPGMPTWNQPYSLTLTSTPGFVE
                     TEHRNNSSGTPDALALRQTILGSSLTAWGRDEDWFTFDVPEDGRAIVDFRFPAGLSAG
                     KAYSLMLHGNSSGLTYAWDVDASASDGALIRNQGVFLRAGRYEVQVLGKDTWAGWGAP
                     YSLNVDVSPGFVEAEPNYTSLNATALPLGKAISGSALSTPSSDIDWFRFEMPEAGAAQ
                     LDFTFPADLGSGQAYTVAVNDAHGQPITTFEVSGPESNGDRLRSLPVPLPAGPAFVSV
                     LALSSWASWGDVYTLSVNAVPVPHSTVTRLAGVNRYETSVAVSRASFASGVSAAYVAS
                     GADFPDALAGAPAAATRGAPMLLVEPSTVPAVVAVELQRLKPGRIVVLGGVGVVSAAV
                     EKELQRLTGASVTRLAGADRYATSVAISKAAFPAGAKTAYLANGSDFPDALSGAPLAG
                     MTKGPVLLVEKNTIPAVVRDELKRLGITEAVMLGGNGVISPAAFDQITLRPLTGRWLG
                     GIDRYGTSAEIAQYAFLQSRPAVAYIASGESFPDALSAAPAAGMNKGPVLLVTPDTIP
                     PATQDALRRLKPSKIVVLGGQGAVSASVESNLAQYAIP"
     gene            complement(3343..4761)
                     /locus_tag="VN041_04565"
     CDS             complement(3343..4761)
                     /locus_tag="VN041_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dTDP-4-dehydrorhamnose
                     3,5-epimerase family protein/NAD(P)-dependent
                     oxidoreductase"
                     /protein_id="HWU28351.1"
                     /translation="MSIEFGKKLVRTETTIPGLVVFDLPVHGDSRGWFKENWQREKMI
                     AAGLPDLGPVQNNISFNDAVGTTRGIHAEPWDKFVSLATGRIFGAWVDLREGPTFGTT
                     FTTELDPSKAIFVPRGVGNAYQTLEADTAYAYLVNDHWSPDAEYAFLNLADETAAIDW
                     PIPLTEAELSDRDRAHPRLEAVTPILPRKILILGAGGQLGRALRTVYGDATHVEYATR
                     ADLDLTSPDLTNARRWRDYGTIINAAAHTAVDAAETTQGRADAWAVNVTAVSALAGIA
                     AANGITLVHVSSDYVFDGSKTVAYTEEDAFSPLGVYGQTKAAGDAIVATVPRHYIVRT
                     SWVIGDGSNFVRTMASLADRGIDPKVVDDQTGRLTFTEDIARGIHHLLETEARYGTYN
                     LTSSGPAQTWADIARDVYRLTGHDPERITRVTTAEYFSSVSGSVAPRPSNSVLDLRKV
                     EQAGFLPHDGVSGLTRYLASRD"
     gene            complement(4758..5756)
                     /gene="rfbB"
                     /locus_tag="VN041_04570"
     CDS             complement(4758..5756)
                     /gene="rfbB"
                     /locus_tag="VN041_04570"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWU28352.1"
                     /translation="MSRLLVTGGAGFIGSNFVHHVVAHTDHHVTVLDKLTYAGNRASL
                     EALPADRVEFVHGDIADAELVDSLFAGTDAVVHYAAESHNDNSLHDPRPFLDTNIIGT
                     YTLLEAARRHGTRLHHISTDEVYGDLELDDPERFTEGTPYNPSSPYSSTKAGSDLLVR
                     AWVRSFGVQATISNCSNNYGPYQHVEKFIPRQITNVIRGIRPKLYGAGRNVRDWIHAD
                     DHSSAVLTILEKGRIGETYLIGADGEKDNKSVVELILELMGQDRAAYDHVADRAGHDL
                     RYAIDSTKLRTELGWRPQYGDFEAGLAATIDWYRANEAWWAPSKDSTEAFYAGKGQ"
     gene            complement(5756..6625)
                     /gene="rfbA"
                     /locus_tag="VN041_04575"
     CDS             complement(5756..6625)
                     /gene="rfbA"
                     /locus_tag="VN041_04575"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWU28353.1"
                     /translation="MKGIILAGGSGTRLHPVTLGVSKQLIPVYDKPMVYYPLSTLMLA
                     GIDDILVITTPHDAAHFERLLGDGSQFGVRITFAQQQSPDGLAQAFTIGADFIGGDSV
                     ALVLGDNLLYGPGLGTQLKRFADIDGGAVFAYWVSEPQAYGVVEFDPEGRVVSLEEKP
                     AKPKSNYAVPGLYFYDNDVVDIARDLQPSARGEYEITDVNRAYLDRGKLHVEVLMRGT
                     AWLDTGTFDQMTDAADYVRTMERRTGMKIGAPEEVAWRQGFISDAELRARAETLVKSG
                     YGTYLLEILERGR"
     gene            6728..8668
                     /locus_tag="VN041_04580"
     CDS             6728..8668
                     /locus_tag="VN041_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HWU28354.1"
                     /translation="MRADWVARVEATEGAAITDSNAPRSRRERRYDGSISDLDPDFEV
                     DEYVEGPRKRRVWPWIVLVIFLLLIAIVVVGGVFAIQALKVKDDLQAAKGKISAVVPL
                     MKKGDTAGVEALSKQVLKLTSDADKIVAGPLWDIASIVPGVGANVVAVSETTKATHIL
                     VRDALPLASSLLPLADPANFKVEGGGINLAPFREAEPKLPQLSAIFDNAKTHIDRIDM
                     KAIHPYIKSNISQIVDIVDTAAPTLSFAQENLPVLLSTLGADGPRNYALLFQNNAEIR
                     ATGGNPGAGAVLTVDNGKVTMRQDQAALNFVLLGPKGRFPQHLADPAEEKLFEGDTWA
                     HSQNYTRTPDFADTAHLVGGLWNQTVGGRLDGVISIDPITLSYMLQVAGPVQVPDETT
                     PVTADNAVKLLLSDTYERFGAKGELADLYFAKVSSAVFTKVMSGGWDPLKMVEQLQKA
                     THEQRIYAWFADEKQQAMATQLGIDGKVTTDNKTVTQTGIYLNDSSHSKLEYYLSSKI
                     MVTCSAEKRTVTTTIELHNAVPRADLSHYTLGARNGRWGQPRTTMMLDVVGIALPGGK
                     LVGSSPEKGARKDQERSGLYKGREAKSMLINVPMGGSKTVSFTSTVPDDATKPLQVRY
                     SPTVGETPVTIDGSCGSMFPAS"
     gene            8853..9299
                     /gene="rplM"
                     /locus_tag="VN041_04585"
     CDS             8853..9299
                     /gene="rplM"
                     /locus_tag="VN041_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWU28355.1"
                     /translation="MTRTYTPKAGQVQRDWIVIDATDVVLGRLASHAAAILRGKHKPT
                     FAPHVDTGDFVVIVNADKVALTGQKLLQKKAYRHSGYPGGLTATSYAELLEKNPVRAV
                     EKAVRGMLPKNTLGRQQLSKLKVYVGAEHPHAAQQPTPYTLDQVAQ"
     gene            9329..9814
                     /gene="rpsI"
                     /locus_tag="VN041_04590"
     CDS             9329..9814
                     /gene="rpsI"
                     /locus_tag="VN041_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWU28356.1"
                     /translation="MAEIQDTTEFPQNFSTETPETEVVESAPRPVLSVPGAAVGRRKQ
                     AIARVRLVPGSGTITINGRTIEDYFPNKLHQQLINDPFTALNLVGAYDVIARISGGGD
                     SGQAGALRLGIARALNNIDAENNRPTLKKAGFLSRDARVIERKKAGLKKARKAPQYSK
                     R"
     gene            9832..11193
                     /gene="glmM"
                     /locus_tag="VN041_04595"
     CDS             9832..11193
                     /gene="glmM"
                     /locus_tag="VN041_04595"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWU28357.1"
                     /translation="MALFGTDGVRGLANGETLTAGLALTLAQATAVVLGQGRIAEARK
                     ATGKRLTAVVARDPRISGHFLSAAVEAGLASSGVDVLDAGTLPTPAAAFLISDIDADF
                     GVMISASHNPAPDNGIKIFARGGVKLPDEVEQRIEAAMAGPMLQPTAGDVGRVRRFAD
                     AEDRYVLHLLASLPHRLDGIHVVLDCANGAASGASPEAFRSAGAKVTVIGADPDGINI
                     NDGVGSTHLDKLAAEVVRVGADVGIAHDGDADRCLAVDADGNVVDGDQIMAILAVAMK
                     SRGKLADDTLVATVMSNLGLHIAMREHGITVRQTSVGDRYVLEDMNANGFSLGGEQSG
                     HVIMSAFATTGDGVLTGLHLVAEMARQKKSLAELASIMTVYPQVMINVKGVDKDAVSD
                     HAGVQDAVKAVETELGDSGRVLLRKSGTEPLVRVMVEAADAETANAHAANLADVVRKH
                     LAL"
     gene            11268..11921
                     /locus_tag="VN041_04600"
     CDS             11268..11921
                     /locus_tag="VN041_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWU28358.1"
                     /translation="MVAPPFDGAGFPPPPPAAPARPSGLPPVHPMAIPPRPPLPAVSA
                     VRPDDVQAGSPLLDADLVALGLTGMSVVDADAPRPPAPLWHRISTWVAVYGVVLALIA
                     FWPVPVDSGSGGFLRWVTAHFPVLTYARIEFGSNILLFVPFGVGLALLLPKLRYLVLP
                     AALLVSLAIESGQAIFLAQRTPSLYDILANVSGAALGLIVLVVVEAVRRRLTDSAGD"
     gene            complement(11928..12140)
                     /locus_tag="VN041_04605"
     CDS             complement(11928..12140)
                     /locus_tag="VN041_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28359.1"
                     /translation="MTTPDALLAQRRIVGIDVARFAAILGMMAGVLAIGAALTLLHRR
                     GPLEVLLGWLSGSSRRRTPQVSPAAV"
     gene            complement(12366..12803)
                     /locus_tag="VN041_04610"
     CDS             complement(12366..12803)
                     /locus_tag="VN041_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28360.1"
                     /translation="MTIVDRRSEAASPAQTRASIAGHRASFDASIERELRRGAALGMS
                     LPSPMWDVLNVLVERELRLATTTTEQTLLTAVPIFGLFRSARCERRLTWLVRRGFVRR
                     ERSTLRPTVAGIAAVRPISSLPGSQRPSMELLRELRRGEIGTV"
     gene            complement(12805..13755)
                     /locus_tag="VN041_04615"
     CDS             complement(12805..13755)
                     /locus_tag="VN041_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28361.1"
                     /translation="MSTPHAIDRLAPDVRDVDEILARLSSMVINPAPVDSRLFAGVGL
                     GDIDILRCDIGPSTIELRQRWRRGHWVLLGRSEGAALNITTADGKQVRAETGEAFLLQ
                     LSHAYRIRTEGSGRLTMVVLPANVLCELPDFVNTVSRRVVVGGMLSTPTWSFLDSLVD
                     QPLPWAPLAAYYAESLITEMLTSIVLTVDTANSRTPSERSLLRRARTHIAAFARDPRL
                     STQTIADSLGISVRSLQRVFANAGESLSAEMRRARVKAAITLLRSAEAEFMTLDEIAQ
                     NVGYVDAVAMRRSFHAAGADQPRNYRSKNPRHGMHREDSL"
     gene            complement(13752..14723)
                     /locus_tag="VN041_04620"
     CDS             complement(13752..14723)
                     /locus_tag="VN041_04620"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HWU28362.1"
                     /translation="MSDSLYALALACLIGVLSASGALKLASPTTTRAGFETLAMPQVL
                     RTPIFIIGVPLLEVALAVSLLLVPAPASIVVGGTCVALFAVFFAVVLRALAKGVSGPC
                     NCFGRLDRTPLSAATVVRNAALLILSVMVALGSARGPLLAIIPGLGSQLPVFQAGLFI
                     LGALAAFIGMSTLKPAPGIASPARPDSKISRHLLHDLFGTAVDARTWHGEERSLLLFV
                     STSCAACHALADSILEVIPAFEASGTRLRIITDVEAGRAISSFPALGARMFHDHDGGL
                     AAEVNITTYPAAVLFDGENGAEIVRGPVVGAQAIAALMEELRLEATV"
     gene            complement(14902..15894)
                     /locus_tag="VN041_04625"
     CDS             complement(14902..15894)
                     /locus_tag="VN041_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28363.1"
                     /translation="MGNSIPNSKEFSRRTIVKGAAWSVPVIAMSTAAPAFAASTQNNL
                     KVETVDCELITLGIAGSNFPGFRYTVTEGIIKKGTQIQITFGGLAAINAGNFELGAAN
                     GISLLNIGGNVATFELTRDYVAGESFTIGLKGFSVNIVGLYTSRIITPDAVQDDNIAI
                     MTAGGVGVGGLLGAFICFDSGDDNVPPVTKVEYDIQVSIQKCDWLVLGSIPTFRIGVV
                     GGTIPQGTTFIMYGSSVATLAPGSWNVDGSVGSALDILGTGSNAWTFRTPRDITPSTP
                     LDVKFTGVGIFAAQTWYLAYLGEDINPANNAAGMQITGVTLGFATVATCQKQTS"
     gene            16052..17173
                     /locus_tag="VN041_04630"
     CDS             16052..17173
                     /locus_tag="VN041_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28364.1"
                     /translation="MNPVTIAAADQIIAIDFDGSVSPEAARIVAETFSHLEFSGDPKH
                     RIVASMSAEAMVPSGGAFARGTTTPKLIRSIADALDGLIMAAFVPDTARGRVDELPQR
                     VALNATALNTPEGAVVIFGGHSRQRAITAMRGTASLIGGDVSVIDRNDRNVSGVAGVI
                     NIGGATNDTARWVSPDAAGVTVHGGPSQVRAFVFLSEAVGEEAGWFPVSTPEAVAMAS
                     GSVPSSSVVTRPLLGLAGIFEMAPAAVVIRYREESQIPTLIDEVLTAPGDKHQVQAAD
                     VRAVEVGTTPLLGDVRRAAMRDAITDGRHMAFCTSQYSVIAVGGIAPMLWDFAEGWIS
                     VDALSTRLIEAIGEPDGDAREHVAAAVEALVIQGVLVRV"
     gene            complement(17500..18966)
                     /locus_tag="VN041_04635"
     CDS             complement(17500..18966)
                     /locus_tag="VN041_04635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWU28365.1"
                     /translation="MSVMSHAASATTRVERQQRRYGTRLAMTDTAVVILAVFGAHLLR
                     FGFNNEPLTIGDRTFDFEIGYVVTSLLFVLGWLVALQMFGTREPSVLDGGSTEYRRIV
                     DATMRVFGGIAILALLFQIQFGRFYLLIALPSGLLLLILTRWLWRNWLRHQRNAGHFL
                     QRAIVIGDREKATHIADKIVREPQSGIRIIGAVTTHGTQEPLLEDVPVLGDFDEVLDI
                     IDSHKVDSVILSTSDAITPKRTRKFGWALDQKRISLIVAPALTDIAGPRIHTRPVAGL
                     PLIHVEYPRFEGRQRIAKRTFDIVGSLGLLILFSPVMIAVALAVKFTSRGPIFYAQER
                     IGLHGKPFKMFKFRSMVVGADDQLKSLLDQQGTSDKPLFKVNDDPRITPVGRFIRRYS
                     LDELPQFANALIGTMSLVGPRPQREAEVALYDEDAHRRLFMKPGITGLWQVSGRSDLS
                     WDDAIRLDLYYVENWSITTDIVVVARTAAAVVKPSGAH"
     gene            19223..20494
                     /locus_tag="VN041_04640"
     CDS             19223..20494
                     /locus_tag="VN041_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU28366.1"
                     /translation="MTAWISIGLSSIAAILAVFACRGVSFREWRSLAFWIPPVVIVWP
                     SIALILGALPIASGVFRGDVVVFGLQVMPSVLVCLLLMARAVVERKRVGGVSALIVSA
                     VVLVGGVFSVVAGQSLLHALVALAVVAGCAFVRNREARAIADALVISVAGMCVLLASY
                     GLLLPGLAVRTDCQQNTAKCDLLGGFLSIGQESGSNAFGITLAMVAAFAVYRLRWSRA
                     VMLTAAVCATILASGARLATACAAASCAIGLLAKARGGRTWAAVALSAATVASLIFAV
                     VPFPPHSFTDRAILWGRARQMILDAPWFGHGLSFWVRDAATQPPPPRSYSPHNLWLDL
                     GVAGGMIAVLVLVVAVLLGLWLSAPEARTGALLILTGIVVVGCFESTFMPFRINPVPA
                     GMVVLLAYLSVNQRTGRLRGLLRRLGRAPAA"
     gene            complement(20430..21599)
                     /locus_tag="VN041_04645"
     CDS             complement(20430..21599)
                     /locus_tag="VN041_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWU28367.1"
                     /translation="MVHLIAWRFIGTLSAALVQFATYVLLARALGVGDYGVFAALFGV
                     GSFITTLTGAGFTHRALRLRAEGDDPSVKRATMVAFRAALGTLLAVVVFLIGTSAELP
                     VASCIVAASFILIDAVTDICQAVMAGADQLGRATMILIAQRLLLLTTAAAFVSTEQPS
                     WLIGAALIATAANVAVTIRGWPRGSTLIKLITTSFSYWRANLASSLSQLETPVIALIG
                     GSAASGLYGAGSRAGGPVNLLAQALLQAATPTLSTALPHERMSLFTQMRHVTWGIVGA
                     ALILAVPAGFAGAWLLGDEYTQAWPFIAGFVVSGALMGVNQAHQAVLFAFDRAASAAR
                     AVAIGAVAALLYAAVAAWALPIWTLILTPVVAQSVMQLGLARAVATGREDGRFSG"
     gene            complement(21605..22498)
                     /locus_tag="VN041_04650"
     CDS             complement(21605..22498)
                     /locus_tag="VN041_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWU28368.1"
                     /translation="MTHGLFGNPRPGRSRVHVLLGHGHGPKSGQDPLSPLLYDTQVAT
                     TNNAVWGRAVIATQLRDPSASVVVSGNPRDDAFDEEVDRERLAALGLDPLCPFVLWLP
                     THRPGQVSAVEQMDKPAARKMEALRQACAARGIVLVTKAHQLDDDRNAEAWGMHVVTS
                     DSLREAGLSFFQLLALSAGLVSDYSSVWVDYLRTGRPFGLLFPDFDLFDTARGFNNPP
                     IAEVAPEAILTDVADVERFIDAVLSHDGKAPSPTSATTARRLGLVTEPGATERVLDAA
                     AEQLARQGIRGLLSPSAERHV"
     gene            complement(22886..23848)
                     /locus_tag="VN041_04655"
     CDS             complement(22886..23848)
                     /locus_tag="VN041_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWU28369.1"
                     /translation="MTFEPKELDRGARTYVAGHRGLVGSAIWRRFEAAGFGDLVGRSS
                     KELDLKDRGRAFDFFREQRPKYVVLAAAKVGGILANSTYPVDFLSENIQIQTNVIDAA
                     LEAGVERLLFLGSSCIYPKFAEQPIREDSLLTGHLEPTNDAYAIAKITGILHIQAVRR
                     QYGLPWISAMPTNLYGPNDNFSPRGSHVLPALIRRYDEAATAGAPVVTNWGTGTPRRE
                     FLHADDMADACLHLLEHYDGDDQVNVGTGRDATISEIADAIAEATGFHGVTEWDTAKP
                     DGTPQKLLDVSRLAELGWTPSISLGDGIAQTVAWYREHREHLRE"
     gene            complement(23845..24873)
                     /gene="gmd"
                     /locus_tag="VN041_04660"
     CDS             complement(23845..24873)
                     /gene="gmd"
                     /locus_tag="VN041_04660"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWU28370.1"
                     /translation="MKRALVTGITGQDGSYLAELLLAKGYEVHGIIRRASTFNTARID
                     HLYVDPHESGARLKLHYGDLRDGSRLVTLLQQVDPDEVYNLAAQSHVRVSFDEPEHTA
                     DTTGTGTVRLLEAVRLSGISTRFYQASSSELYGATPPPQSETTPFYPRSPYAAAKLYS
                     FWITKNYREAYRMYAVNGILFNHESPRRGETFVTRKITRAVARIKAGQQSDLYLGNLD
                     AIRDWGYAAEYVEGMWRMLQADAPDDYVLATGVGYSIRDFLDAAFSHVGMNWQDYVRF
                     DERYLRPTEVDALIGDPSKARDALGWEATVDGLDLARLMVEADVQALAHEGRPWVDTV
                     TLDGWTRR"
     gene            complement(25010..26476)
                     /locus_tag="VN041_04665"
     CDS             complement(25010..26476)
                     /locus_tag="VN041_04665"
                     /inference="COORDINATES: protein motif:HMM:NF007773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HWU28371.1"
                     /translation="MTNLQRATLSGSFWVAISVLFAAVVQLAQLASVARVLEPTQVGV
                     VAASLVILALADTIANMGIANSIIQRQNASRDELSSLYWLNVLVGIVIAVALYVSAPL
                     FEWFFRMPELTALVHVIALGFIFSPHGQVPRGILERRMAFRSVAVIEMVGAATILAAT
                     LVLLFLGAGPVAVAAAYAFSVSLKALLFVYASRREFFPRFHFRLVETTRFLSFGLITS
                     LDMIVSFFAANIGSFAVGRLVSPTALGGYNLAFTYAVNTPARLNGVVTRVAFPAMSQI
                     QSDRSRQARAIRRVIETVTIVNAPLLVTLAIVALPFTEVFFGPNWLWVAALIQVLSGV
                     GLTRAMGNPMGAILMALNRMGVGLIVNVIKSSVHIAVVIFGAIWGGVFGAAWAAVIMG
                     VVTLATNVVLLKTLAGIPARTGILDHVRPLLYSIPLAIVGVGMMWVTSWLGMIPLVTL
                     IVVCAACGVTYLCTLMVTRHFLVQTLTAFLRPSSGSSV"
     gene            complement(26473..27450)
                     /locus_tag="VN041_04670"
     CDS             complement(26473..27450)
                     /locus_tag="VN041_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HWU28372.1"
                     /translation="MSVLTLIIGLDRNYRGAELEVQLQAIGIRNQRSPGVEGEYGGHP
                     LTGYASTEGARVVYGRELRSGEIGCALAHRESYLTFLSSAADWALIFEDDARIVRPDA
                     LAAVFAHLTALPAFDEPAILMLYGRQMIGDPRSKLMLGEVEVQQLILTPMTATAYFIN
                     RTAAQHIIDTGLPLRNPADWPVSVEGTVRFAGAYPWIAMPDEVGAPSSIGERGEARSP
                     VRIFLNRLDSLFFVKFLKKRKFYRSSREYWDWEVRRRFMQLLLRQHPYRVPDGSTTLP
                     EAAALTLAVDRVFRGHHDRSLIEYLSRDLTKALDRPDATERAAEADLGS"
     gene            complement(27516..28517)
                     /locus_tag="VN041_04675"
     CDS             complement(27516..28517)
                     /locus_tag="VN041_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWU28373.1"
                     /translation="MGLLWLTHVVNTQSVSSGITFVTLVAVIAVAVWSIDTQPEDLVV
                     LGWLGFAIAAVSLLMAATTDIAWTDETEDSKALIGHAVLAGFFPQMNPLGMSMAIALP
                     FTLLFKRRLLRVVGFASVALTLLMASSRTAVIATGIALVVGLVLRWIPSKSRPIVGYL
                     ASGIVVVVSIIVPLRSDDSAFTSRGAVWRASFDLFPERPFWGYGPGVYGLEGEVSRIV
                     NGAYWHGHNMLITFALIGGIVALIALALILIPAIHAALVHAKAGAILPMMAVMTILAL
                     GIAEVPIRPSEFDGVAWISWLCLFAIGNLSPRPEQVAPAFDSDAERPPRNWRNASRM"
     gene            complement(28967..30175)
                     /locus_tag="VN041_04680"
     CDS             complement(28967..30175)
                     /locus_tag="VN041_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWU28374.1"
                     /translation="MAQVGSITDVAALMPGAERFDSTGGAIASWVRETYSRSKHSTLV
                     ASPRATESFAAPVPIANVRLYDAYERVLSSTSRLLAPRIGKSFQVVYNRLLWDRLWVR
                     SAYRRIREAKLIHIHNRPHYARELREAGFTGAVILHMHNDLRTYVTMDESAEVFSHID
                     YVAFCSDFVRDRAVESFPALPPSAVVANGVSRSQIDRRVERQPSATLRLIYAGRIIPE
                     KGPLEAVAICGELRRRGIKANLDLVGGTGTGSENGPTQYLSQVEAAVSALNRREGDTV
                     ARFLGPRPHDEVFDLFAANDIFLLPCSWEEPFGMVALEAMAKGCVPVVSDRGGLPDVV
                     QDGGVAVRIPEDQREVPVVFADAIERVNTPDDLTRLRACGWNRAEQLTWESVATSFDD
                     LIEIALRAVS"
     gene            complement(30187..31074)
                     /locus_tag="VN041_04685"
     CDS             complement(30187..31074)
                     /locus_tag="VN041_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU28375.1"
                     /translation="MTSTEWTVSAVIPTIGRPELRLAVESALRQTEHLLEVIVAADTD
                     HELDLPDDSRVIVIRTGPRAGGNVARMAGIRAARGELIALLDDDDEWMPDKLARQILA
                     VQSAGAHDVPWISSTLVLEPSGRAWPERRLAPGEPLPSYLFRKDRVKAGQGAMHTSTL
                     LFPRSLAIEIPFDESLRFHQDIDWLIRVDRDATALRVTQVDAALTQLGEGGGSVSRGI
                     APERSLEWAHDALAHVDRRVRGDFLVTVTYFQACRHRRPGAALRVLRSAYTWGRPGVR
                     TAPSVLVLPLKVLTGKGIA"
     gene            complement(31071..32303)
                     /locus_tag="VN041_04690"
     CDS             complement(31071..32303)
                     /locus_tag="VN041_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWU28376.1"
                     /translation="MSRDTEFRMPEHSVAVSSAFDENAIRAIAQRASRHGILTRRAIR
                     DQAPLLSLARGAARVLPRSKTISGFVAKQSARVATGALPEDVRLSPGSEFIRVLGGRV
                     RSPLTHTLGNATWKVAYDRAARHVDFGDARTLISMPGSSLETFTRNSDRRLVLHEIDA
                     HPRVRNERLEAFYGARRAKAETYPDWFVDRIETELDLADDVLVPGRVVAGQMRSNGVE
                     DSKIIQIPYGVDPGVFRPPTEQIPTRRARPRVVFTGQICLRKGVGFLLEAVKGLQLDL
                     VLVGSVFDRQLVRALPSNVRLLGTLSATQLARLYAESDAFVLPSIEDNFALVVTEAEA
                     AGLPVITTFETGSHELLGPAHTILRAGDVASLRDALTAHEMLAWDERRENADLAGSRH
                     ARSWDDYGDAVLREVSTP"
     gene            complement(32300..33364)
                     /locus_tag="VN041_04695"
     CDS             complement(32300..33364)
                     /locus_tag="VN041_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWU28377.1"
                     /translation="MIAINCRFLTQDLTGVQRFAEEITAELARTRDDVRLFAPPGALR
                     HTALGGREVEQVGTASGHRWEQWDLPRVLRRDHGSPLLLSLMNTGPVLYRNQVVTHHD
                     VTYVRFPQTYTKRFRIAYRMLSSLTLHRARQVITVSEFSRREIVDVYGIDLEKIRVVP
                     NAAGSAFRRPHLESRPAYFLAVASFLPHKNIDRMVDAFERYRAESGSTTALRLVGSAR
                     PTSMARSDGSPRATPGVELLGRVDDQLLQELYAGARGFIFPSLYEGFGVPPLEAQSAG
                     APVAAADIPPLREALGESALFFDPGDEGAMISSLRILDSEAEVRDRLRSAGADNVDRY
                     SWSGSAERVSASLDDLHGDR"
     gene            complement(33361..34461)
                     /locus_tag="VN041_04700"
     CDS             complement(33361..34461)
                     /locus_tag="VN041_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU28378.1"
                     /translation="MTGLLVHEWIESSGGAENVLEAMARAFPDADIQCLWDDAPHRFV
                     DRSVRETWLARTPLRRHKALALPFTVGAWRRLPASRDYEWMLVSSHLFAHHATLAGRD
                     VPKYVYAHTPARYIWNPELDERGNSLAVRAVSPLFQRIDRRRAQEATAIAANSAFVRD
                     RIAQAWDREATVIHPPVAVERILSQSDWRTQVADADERRLLEQLPTPFILGASRFIPY
                     KRLDLVIAAGERTGLPVVIAGRGPEEQRLRTIAATAKVPVRIVVSPSDALLYALMQLA
                     SVFVFPPVEDFGIVPVESQAAGTPVVTGPVGGQVETFVDGVSGVAAASTAATDLADAV
                     RRAASLPAFEPRDATARFDEPAFREHIDRFVS"
     gene            complement(34461..>35050)
                     /locus_tag="VN041_04705"
     CDS             complement(34461..>35050)
                     /locus_tag="VN041_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWU28379.1"
                     /translation="GHDARIGRKFLGAGIGFGGGCLPKDIRAFAARAEELGRGESVNF
                     LREVDAINLRRRERAVQLVVDGLGGSVYGKKVAVLGAAFKPHSDDVRDSPALDVAVRL
                     NGLGADVVVTDPQAIENARRLHPQLTFEADRDETIRGAAAVVMVTEWDEYRRELSPEH
                     AGSLTDGRVVVDGRNGLDAAAWRAAGWSYYGMGRP"
BASE COUNT         6213 a        11149 c        11631 g         6057 t
ORIGIN      
        1 agcgcgccga actgctgggg ctcccgctgg cgggcacgat gatctcgctg atgctggcgg
       61 gctgcgccgc cgtgatgctg gtcgtcaccg gagcggccgt gtcggtcggc ctgccgaacc
      121 tcgacgcggg ggccgcatgg ctgaccggcg ggtcgctgct gctggccgcg ctggtgtccg
      181 ggatgctcgc atcccgacgg gaagtgcggc ttcggatgct ggtgcgcgac gccctgatgc
      241 cgggtgacgt ctcgggcgat gacgacgtgg acgcataacc ggccgcactc gccatttcaa
      301 ccccacgacg cgttcagcgt gcggcggtaa cgtgaacgca tgaggctcat ctcgtacaac
      361 ctgcgcaagc atcgggcggc atccgagctg gtggcgctgg tcggcagaca tgaccctgac
      421 atcctgtgcc tgcaggaatg cgacaccacg gcgctgcccg aatccatcga cgacctctcg
      481 ctcgtgcggg ccaccagcgg caaccggctc ggcctggcga tgtacctgcg cagcaatcag
      541 ttccgggtgg agcgggtgcg cacgatcggc ctgaagaagt ccctgcacga ccgcgtgctc
      601 aagcccgccg aggagcgggt gctcggggcg ctcctgacga acatcgacac gggccgccag
      661 ctgatcgtgg catcctttca cgcagccccg ctgaccgcgc tgaactcgct gcgccgcaac
      721 cagatccgcg ccggtctcga cgcgctgcag gcgctcgctc ccggcaagcc ggtgctgatg
      781 gtcggtgact acaactatcc ggtgttcaag gagaacctcg ggcagacggt gcgcgagcac
      841 ggctaccggc tgaccctcag cgacgaccgc acctacaccc ggtaccgcgt gttccgcggg
      901 cactacgact tcgcgacttc gaacggcttc gacatcgccg acatcaccac gctcccgcag
      961 ggcaccagcg atcaccgtcc gatcctgatc acggccgagg ccgcgtagcg ggtctcaatc
     1021 ccagaccttc ggatgggctg aaccggaata ccccgctacg atgagcgcgg atgaacgatg
     1081 cggtcatcca cttcgcgcga cgtctgaggg gacattcgca tggatctgtc ggatatgtcg
     1141 cgaggccgga ggatggtcgc ggtcatcgcg tcgaccgtgc tcgtcgctgg cctgctggtc
     1201 gtcgctggcg gtgcctcggc gagtgcggcc acggccacgg aggtcgaatc gaacgacgag
     1261 gtgtccacgg cgaatccatt gccgctcggc acgacaatgc acggcaccgc ttacagctcg
     1321 tccgacaagg accaggacta ctacgtcttc gacgtaccgg agagcggtca gctgcacgtc
     1381 gacctgcagt tcccagccgg cttgcagggg ctgtacgcct atgaggtcga gattcagggt
     1441 gctcgtggtg acgccgaaac gtacttccgt tgggtgccga cgccggactg gtctgacgga
     1501 acccgcctgc gcagtcaagc gcctttcctg caggcggggc gctactggct gctgatcgct
     1561 gcggccccag gaatgccgac ctggaaccag ccgtactcgc tcacgctcac gtcgactccg
     1621 ggcttcgtgg agaccgagca tcgcaacaac agttcaggca cccccgatgc tctcgcactc
     1681 aggcagacga tcctaggatc cagtcttacc gcgtggggac gcgacgagga ttggttcacc
     1741 ttcgacgtac cggaggatgg ccgggcgatc gtcgacttcc gattccctgc gggcctgtcg
     1801 gcaggaaagg cgtattcgct gatgctgcac gggaacagca gtggtctgac atacgcatgg
     1861 gacgtcgatg cctcggcatc ggatggtgcg ctcattcgca accagggcgt gttcctgcgt
     1921 gcgggccgct atgaggtgca ggtgctgggg aaggatacat gggcgggatg gggagccccg
     1981 tactcgctga acgtcgatgt gtcgcccgga ttcgtcgaag ctgagccgaa ttacacctcg
     2041 ctcaacgcca cggccctgcc ccttggtaag gcgatcagcg gttcggcgct gtcgactccc
     2101 tcgagcgaca tcgactggtt ccggttcgag atgccggaag cgggtgccgc acagctcgat
     2161 ttcacctttc cagcggacct cggcagcggt caggcgtaca cggtcgcggt caacgacgct
     2221 catgggcagc cgatcacgac ctttgaggtg agcgggcctg aatccaacgg cgaccgtctg
     2281 cgctcccttc cggttcctct gccggccggg ccagcgttcg tcagcgtgct ggcgctcagc
     2341 tcctgggctt cttggggtga tgtctacacg ctttcggtga acgcggttcc cgtgccgcac
     2401 tcgacggtca cccgcttggc tggggtcaac aggtacgaga catccgtggc tgtgtcccgg
     2461 gcgtcgttcg cgtcgggagt ctcggctgcg tatgtcgcca gcggcgccga cttcccggat
     2521 gctctggctg gggctccggc cgcggcgaca cggggtgctc cgatgctgct cgtggagccg
     2581 tcgacggtcc ccgcagtcgt tgctgtcgag ttgcagaggc tcaagccagg acggatcgtg
     2641 gtcctgggcg gcgttggagt cgtgagcgct gcggtggaga aggagctgca acgattgacc
     2701 ggcgccagcg tgacgcggct cgccggcgcc gaccggtatg cgacatccgt ggccatctcc
     2761 aaggcggcct ttccggccgg cgccaagacc gcgtatcttg ctaacgggtc agatttcccc
     2821 gacgcgcttt cgggggcgcc tctcgcgggc atgaccaagg gacccgtgct tctggtggag
     2881 aagaacacga ttccggccgt cgtgcgtgat gagctgaaga ggctgggcat cacagaggcc
     2941 gtgatgctgg gaggcaacgg cgtgatcagc ccagctgcct tcgaccagat cacccttcgc
     3001 ccgctgacgg gacgctggct gggcgggatc gaccggtatg ggacatccgc cgagatcgct
     3061 cagtatgcgt tcctgcagag cagacctgcg gtggcgtaca tagcctccgg cgagagcttt
     3121 cccgacgccc tctcagcggc gcccgccgca gggatgaaca agggccccgt gctgctcgtg
     3181 acgccagaca ccatcccgcc cgccactcaa gacgcgctcc gacgactcaa gccctcgaag
     3241 atcgtggtgc tcggcgggca gggcgcggtg agtgcatcag ttgagagcaa cctggcgcag
     3301 tacgcgatcc cctgactcag cgtccgaagc ctctgaagca cgtcaatccc gtgaagcgag
     3361 gtacctcgtc aggccggaaa cgccatcgtg gggtaggaaa ccagcctgct cgactttcct
     3421 cagatcgagg acgctgttgc ttgggcgcgg tgcaacggac cctgacactg acgagaagta
     3481 ctcggccgtg gtgactcggg tgatgcgctc ggggtcgtga cctgtgaggc ggtagacgtc
     3541 ccgggcgatg tcggcccagg tctgcgcggg gccgctgctg gtgaggttgt atgtcccgta
     3601 ccgggcttcc gtctcgagca gatggtggat gccgcgggcg atgtcctccg tgaaggtgag
     3661 ccggccggtc tggtcgtcga cgaccttcgg gtcgatgcct ctgtcggcga gggatgccat
     3721 agtgcgaacg aagttcgacc cgtcaccgat gacccaggat gtgcgcacga tgtagtgccg
     3781 gggcacggtg gcgacgatcg cgtcgccggc ggccttggtc tggccgtaga cgccgagggg
     3841 gctgaacgcg tcttcctctg tgtacgccac cgtcttggag ccgtcgaaca cgtagtcgct
     3901 ggagacgtgg acgagggtga tgccgttggc ggccgcgatg ccggccaatg cggacacggc
     3961 ggtgacgttg acggcccagg cgtccgctcg tccctgcgtc gtctctgctg cgtcgacggc
     4021 ggtgtgggcg gcggcgttga tgatcgtgcc gtagtcgcgc cacctccggg cgttcgtgag
     4081 gtctggcgag gtgaggtcga ggtcggcgcg ggtggcgtac tcgacgtggg tcgcgtcgcc
     4141 gtagacagtg cggagcgcgc ggccgagctg cccgccggca cctaggatga gaatcttgcg
     4201 cggcaggata ggggtcacag cttcgagccg gggatgcgcg cggtccctgt cggagagctc
     4261 ggcctctgtg agcgggatgg gccagtcgat cgcggccgtc tcgtcagcga gattcaggaa
     4321 ggcgtactcg gcgtcaggcg accagtggtc gttgacgagg tacgcataag cggtgtccgc
     4381 ttcgagcgtc tgataggcgt tgcccacgcc ccgagggacg aagatcgcct tcgacgggtc
     4441 gagttccgtc gtgaacgtcg taccgaaggt gggaccctca cgcaggtcga cccaggcgcc
     4501 gaagatgcgt ccggtcgcga gggagacgaa tttgtcccag ggctctgcgt gaatgccacg
     4561 cgtggttccg actgcgtcgt tgaaggagat gttgttctgc accggtccga gatccgggag
     4621 tccggcggcg atcatcttct cgcgctgcca gttctccttg aaccacccgc gactgtcgcc
     4681 gtgcacgggc aggtcgaaga cgacgagccc aggaatcgtc gtctccgtgc ggaccagctt
     4741 cttgccgaac tcgatgctca ttgtcccttg cccgcgtaga acgcctccgt gctgtctttg
     4801 gacggggccc accaggcttc attcgcccgg taccagtcga tggtcgcggc aagaccggct
     4861 tcgaagtcgc cgtactgcgg cctccagccg agctcggtgc ggagcttggt ggaatcgatc
     4921 gcgtaccgca gatcgtgccc ggcgcggtcg gccacgtggt catacgccgc ccgatcctgg
     4981 cccatcaatt ccaggatgag ctcgacgacg gacttgttgt ccttctcacc gtcggcgccg
     5041 atgaggtagg tctcgccgat ccggcccttc tcgaggatgg tgagcacggc tgaggagtgg
     5101 tcgtcggcgt ggatccagtc acgcacgttc cgtcctgcac cgtagagctt cggccggatg
     5161 ccgcggatca cgttcgtgat ctggcggggg atgaacttct ccacgtgctg gtagggcccg
     5221 tagttgttag agcagttcga gatcgtggcc tgaaccccga aggagcgcac ccaggctcgg
     5281 acgagcaggt cggagccggc cttggtcgac gagtacggcg acgacgggtt gtacggggtg
     5341 ccctcggtga accgctccgg atcatcgagc tccaggtcgc cgtacacctc gtccgtggag
     5401 atgtgatgca gtcgagtgcc gtggcggcgt gcggcctcca gcagcgtgta ggtgccgatg
     5461 atgttcgtgt ccaggaacgg ccgagggtcg tgcagcgagt tgtcattgtg cgactcggcg
     5521 gcgtagtgca ccacagcatc cgtgccggcg aagagcgagt ccacgagctc ggcatccgcg
     5581 atgtctccgt gcacgaactc cacacggtcg gcgggcagcg cttccaagga cgcacggttg
     5641 cccgcgtagg tgagcttgtc cagcaccgtc acatggtgat cggtatgcgc gacgacgtgg
     5701 tggacaaagt tggagccgat gaatccggcg ccacccgtga ccagcagccg cgacatcagc
     5761 gtcccctctc caggatctcg agcaggtacg tcccgtagcc cgacttcacc agcgtctcgg
     5821 cgcgcgcgcg cagctcggcg tccgatatga acccctgccg ccaggcgacc tcctctggtg
     5881 cgccgatctt catgcccgtc ctgcgctcca tcgtgcggac gtagtcggca gcatccgtca
     5941 tctgatcgaa cgttccggtg tccagccacg ccgtgccgcg catgagcacc tcgacgtgca
     6001 gcttgccgcg atcaaggtat gcacggttca catcggtgat ctcgtactcg cctcgcgcag
     6061 aaggctgcag atctcgagcg atgtcgacga catcgttgtc gtagaagtac agtccgggaa
     6121 ccgcgtagtt gctcttcggt ttcgccggct tctcctccag cgatacgacg cgaccctcgg
     6181 gatcgaactc cacgacgccg tacgcctgcg gttctgacac ccagtatgcg aagacggcac
     6241 cgccgtcgat gtccgcgaat cgcttcagtt gagtgccgag ccccggccca tagagaaggt
     6301 tgtcgccgag cacgagggcc actgagtcgc cgccgatgaa gtccgcgccg atcgtgaatg
     6361 cctgcgccag gccgtcgggc gactgctgct gggcgaacgt gatccgcacg ccgaactgcg
     6421 agccgtcacc gagcaggcgc tcgaaatgcg cagcgtcgtg cggcgtggtg atcacgagga
     6481 tgtcgtcgat gcccgccagc atcagcgtcg acagcgggta gtacaccatc ggcttgtcgt
     6541 acaccggtat cagttgtttg gagacgccca gcgtgaccgg atgcagacgg gtccccgagc
     6601 cgcccgcgag aatgatcccc ttcatgtgac cagattgcca cgatgcgctg ccctgtggcg
     6661 gtgccagccg acaccagttc tcatgatctc gtcagggcgc gccccaggca tactcagtat
     6721 cctgagggtg cgtgccgact gggtggcgcg cgtcgaggcg accgaggggg ctgccatcac
     6781 cgattcgaac gcaccacgca gccgacgcga gcgacgctac gacgggtcca tcagcgacct
     6841 cgacccggac ttcgaggtcg acgagtacgt cgagggcccg cgcaagcggc gcgtctggcc
     6901 gtggatcgtc ctggtcatct tcctgctgct gatcgcgatc gtggtggtcg gcggcgtgtt
     6961 cgcgatccag gcgttgaagg tcaaggacga cctgcaggcc gcgaagggca agatctcggc
     7021 ggtcgtgccg ctcatgaaga agggcgacac cgccggcgtc gaggcactga gcaagcaggt
     7081 gctcaagctc accagcgacg ccgacaagat cgtggccggt ccgctgtggg acatcgcgag
     7141 catcgtgccg ggcgtcggcg cgaacgtcgt cgccgtcagc gagaccacca aggcgacgca
     7201 catcctggtg cgcgacgccc tgcctctggc atcctcgctg ctcccgcttg ccgaccccgc
     7261 gaacttcaag gtcgagggcg gcggcatcaa cctcgcaccg ttccgcgagg ccgagccgaa
     7321 gctgccgcag ctgagtgcga tcttcgacaa cgcgaagacg cacatcgaca ggatcgacat
     7381 gaaagcgatc cacccgtaca tcaagtcgaa catcagtcag atcgtcgaca tcgtcgacac
     7441 ggccgcaccc actctctcgt tcgcgcagga gaacctgccc gtgctgctgt cgaccctcgg
     7501 tgccgatggc ccgcgcaact acgcgctgct gttccagaac aacgccgaga tccgtgcgac
     7561 gggcggcaac cccggcgcgg gcgccgttct cactgtcgac aacggcaagg tcacgatgcg
     7621 gcaggaccag gcggctctga acttcgtgct gctgggcccg aaggggcgat tcccgcagca
     7681 cctcgcggat ccggcggagg agaagctctt cgagggcgac acctgggcgc actcgcagaa
     7741 ctacacgcgc acgcccgact tcgccgacac cgctcacctg gtcggcggcc tgtggaatca
     7801 gacagtgggc ggtcggctgg acggcgtcat ctccatcgac cccatcacgc tctcctacat
     7861 gctccaggtg gccggccccg tgcaggtgcc ggacgagacc acgcccgtga ccgcggacaa
     7921 cgcggtgaag ctgctgctca gcgacaccta cgagcgtttc ggcgccaagg gcgagctggc
     7981 cgacctctac ttcgccaagg tctcgtcggc ggtgttcacc aaggtgatga gcggcggatg
     8041 ggatccgctg aagatggtcg agcagctgca gaaggccacg cacgagcagc gtatctacgc
     8101 ctggttcgcg gacgagaagc agcaggcgat ggcgacgcaa ctgggcatcg acggcaaggt
     8161 caccaccgac aacaagaccg tgacgcagac cggcatctac ctgaacgact cgtcgcactc
     8221 caaactggag tactacctgt ccagcaagat catggtgacc tgctccgcgg agaagcgaac
     8281 ggtgacgacg acgatcgagc tgcacaacgc cgtcccgcgc gcggatctga gccactacac
     8341 gctgggcgct cgcaacggcc gctggggtca gccccgcacc acgatgatgc tcgacgtcgt
     8401 gggcatcgcc ctgccgggag gcaagctcgt cggctcctca cccgagaagg gggcgcggaa
     8461 ggaccaggag cgcagcgggc tctacaaggg gcgcgaggcg aagagcatgc tcatcaacgt
     8521 gccgatgggc ggctccaaga cggtgtcgtt cacatcgacc gtccctgacg acgccaccaa
     8581 gcccctgcag gtgcggtact cgccgaccgt gggcgagacg cccgtcacca tcgacggctc
     8641 ctgcggctcg atgttccccg cctcctgaat tgggggagca gcggaggatg ctgtaagcta
     8701 accctttggt gcctgtgctt cggctcgccg agacaccggt acgacgaacg tgagccctcc
     8761 actggcgtgt tcccggtctc gatcagaggc aggaaccacc ccggagtggg attcacgaac
     8821 ctctccgttc gattcaagaa agcagcacta tcgtgacgcg cacttacacc ccgaaggccg
     8881 ggcaggtcca gcgtgactgg atcgtcatcg acgccaccga cgtcgttctc ggtcgcctgg
     8941 cttcgcacgc cgccgcaatc ctgcgcggca agcacaagcc gaccttcgct ccccacgtcg
     9001 acaccggcga cttcgtcgtc atcgtcaacg ccgacaaggt cgccctgacc ggtcagaagc
     9061 tcctgcagaa gaaggcctac cgccactcgg gttacccggg cggtctgacg gccacctcct
     9121 acgccgagct cctcgagaag aacccggttc gcgccgtgga gaaggccgtc cgcggcatgc
     9181 tcccgaagaa caccctgggc cgccagcagc tctcgaagct gaaggtgtac gtcggtgccg
     9241 agcacccgca cgccgctcag cagcccacgc cgtacaccct cgaccaggtc gcgcagtaag
     9301 cgccccttag atttcaaagg acatactcgt ggctgaaatc caggacacca ccgaattccc
     9361 gcagaacttc tcgaccgaga cccccgagac cgaggtcgtg gagtccgctc cccgccccgt
     9421 gctctccgtg ccgggcgctg ccgtgggccg tcgcaagcag gccatcgccc gcgtgcgcct
     9481 ggttcccggt tcgggcacca tcacgatcaa cggtcgcacg atcgaggact acttcccgaa
     9541 caagctgcac cagcagctga tcaacgaccc gttcaccgcc ctcaacctcg tgggtgcgta
     9601 cgacgtcatc gcccgtatct cgggtggcgg cgactcgggt caggccggcg ccctgcgcct
     9661 gggcatcgct cgcgcgctga acaacatcga cgccgagaac aaccgcccga ccctcaagaa
     9721 ggcaggcttc ctgtctcgtg acgcccgcgt catcgagcgc aagaaggccg gtctcaagaa
     9781 ggcccgcaag gctcctcagt actcgaagcg ctgatccgcg cagttcgtca tatggcactg
     9841 ttcggtacgg acggcgtgcg gggtctcgcc aatggcgaga ccctcaccgc cggacttgcg
     9901 ctcaccctgg cccaggcgac tgccgtcgtc ctgggccagg gccgtattgc cgaggctcgc
     9961 aaggccacag gcaaacgtct caccgcggtc gtcgctcgcg acccgcggat ctccggtcac
    10021 ttcctcagcg cggctgtcga ggcgggtctc gcctcctccg gtgtggacgt gctggatgcc
    10081 ggcaccctgc cgaccccggc ggctgccttc ctgatctcgg acatcgatgc cgacttcggc
    10141 gtgatgatct cggcatccca caacccggct cccgacaacg gcatcaagat cttcgcccgt
    10201 ggcggagtga agctgcccga cgaggtcgag cagcgcatcg aagccgcgat ggccggtccc
    10261 atgctgcagc cgaccgccgg tgacgtcggt cgcgtgcgcc gcttcgcgga tgccgaggac
    10321 cgctacgtcc tgcacctgct ggcgtcgctg ccgcatcggc tcgacggcat ccacgtcgtt
    10381 ctcgactgcg ccaacggcgc ggcatccgga gcgtcgcccg aggcgttccg cagcgccggc
    10441 gcgaaggtga ccgtcatcgg ggcggacccg gacggcatca acatcaacga cggcgtgggc
    10501 tcgacccacc tcgacaagct ggccgccgag gtcgtgcgcg tcggggccga cgtgggaatc
    10561 gcgcacgacg gcgacgccga ccgctgcctg gcggtcgacg ccgacggcaa cgtcgtcgac
    10621 ggtgaccaga tcatggcgat cctcgcggtg gcgatgaaga gccgcggcaa gctcgccgac
    10681 gacacgctgg tcgcgaccgt gatgagcaac ctgggcctgc acatcgccat gcgcgagcac
    10741 ggcatcaccg tgcgccagac cagcgtcggc gaccgctacg tgctcgagga catgaacgcc
    10801 aacggcttct cgctgggcgg cgagcagtcg ggccacgtga tcatgagcgc gttcgccacc
    10861 acgggtgacg gcgtgctgac cggcctgcac ctggtcgccg agatggcgcg ccagaagaag
    10921 tcactggccg agctggcatc gatcatgacg gtctacccgc aggtgatgat caacgtgaag
    10981 ggcgtcgaca aggacgccgt ctcggatcat gccggtgtgc aggacgccgt gaaggccgtc
    11041 gagaccgagc tgggggactc cggccgcgtg ctgctgcgca agtccggcac cgagcctctc
    11101 gtgcgggtga tggtcgaggc cgcggatgcc gagacggcga acgcccacgc cgcgaacctc
    11161 gccgacgtgg tgcggaagca cctggcactc tgaggcattg tgcggcggct cacagggccg
    11221 ccgcataagc tcatcctggg gatgacgaac ctggggaggg ggagctgatg gtcgcaccgc
    11281 ctttcgacgg cgccggcttc ccgccgccgc ctcctgccgc tcccgcgcgt ccgtccggcc
    11341 tgcctcccgt gcatccgatg gcgattccgc cgaggccgcc cctgcccgcg gtgtccgcgg
    11401 tacggcctga cgacgtgcag gccggctcac ctttgctgga tgccgatctg gtcgccctcg
    11461 gactgacggg gatgtccgtg gtcgacgcgg atgctccccg gccaccggcg ccgctgtggc
    11521 atcggatctc cacgtgggtg gccgtgtacg gcgtcgtgct ggcgctgatc gcgttctggc
    11581 ccgttcctgt cgactcgggc tccggagggt tcctgcgctg ggtcaccgcg cacttcccgg
    11641 tgctcacgta cgcgcgcatc gagttcgggt cgaacatcct gctgttcgtg ccgttcggtg
    11701 tcggactggc actgctgctg ccgaagctgc ggtatctcgt gctgccggcg gctctgctcg
    11761 tgtcgctggc catcgagtcg gggcaggcga tcttcctcgc ccagcgcacg ccgagtctct
    11821 acgacatcct cgcgaacgtc tcaggtgcgg ctctcggact catcgtgctg gtcgtggtcg
    11881 aagcggtgcg ccggcgcctc accgactctg caggggactg accgacgtca gaccgccgcg
    11941 ggtgacacct gcggcgtccg acgcctgctg gacccgctca gccagcccag cagcacttcg
    12001 agcgggccgc gtcggtgcag cagcgtcagc gcggcgccga tggcgagcac gccggccatc
    12061 atgccgagga tcgccgcgaa tctggcgacg tcgatgccga cgatgcggcg ctgggcgagc
    12121 agagcatcgg gcgtcgtcat ggctctcatc ctgccgagtg aaggtgactc gcccatccga
    12181 accacggact cgcgtaatga atcacgcttc atcgtgtctc ttccatgggt gttgtgcgct
    12241 cgtagctcgg gggagcatcg agcccactcc catcccaggg gaggacccgc ggccagttgg
    12301 gggactccgc cgcgggagca cccgacgggg cgccgatccc tcaatcggcg ccccgtctct
    12361 cctggtcaga cggttccgat ctccccgcgg cgcagttcgc gcaagagctc catggaaggg
    12421 cgctgcgatc ccggaagaga tgagatgggc cggaccgccg cgatgcccgc cacggtgggg
    12481 cggagcgtgg agcgctcgcg acgcacgaag ccgcgacgga cgagccaggt cagacgtcgt
    12541 tcgcagcggg cgctgcggaa gaggccgaag atcggcaccg cggtgagcag ggtctgctcg
    12601 gtggtggtcg tggcaagacg cagctcgcgc tccacgagca cgttgagcac atcccacatc
    12661 gggctgggca gactcatgcc cagagccgcc ccgcggcgca gctcgcgctc gatgctggca
    12721 tcgaagctcg cgcggtggcc ggcgatggac gcgcgagtct gggcgggtga cgctgcctca
    12781 gagcgtcggt cgacgatggt catactagag agagtcctct cggtgcattc catgacgcgg
    12841 attcttcgat cgatagttgc ggggctggtc tgccccggct gcgtgaaagg atcgcctcat
    12901 ggccacggcg tccacgtagc cgacgttctg cgcgatctcg tcgagcgtca tgaattcggc
    12961 ttcggcggag cggagtagtg tgatggccgc cttcacacgc gctcggcgca tctccgcgct
    13021 gagactctca cccgcgttcg cgaacactcg ttgcaggctg cgcaccgaaa tgccgaggct
    13081 gtcggcgatg gtctgggtcg agagcctcgg gtcacgtgcg aacgcggcga tgtgggtgcg
    13141 cgcgcgacgc agcagcgagc gttccgatgg cgtcctgctg ttcgccgtat cgacggtcaa
    13201 cacgatcgac gtgagcatct ccgtgatcag gctttcggcg tagtaggccg ccaacggagc
    13261 ccaggggagg ggttggtcta cgagcgagtc cagaaaggac caggtaggcg tgctcagcat
    13321 cccgccgacc accactcttc tcgacaccgt gttcacgaag tcgggcaact cgcacaggac
    13381 gttggccggc agcaccacca tggtgaggcg tcccgatccc tcggtacgga tccgataggc
    13441 gtgggacagc tgcaacagga aggcctcgcc tgtctcggcg cgcacctgct tgccgtcggc
    13501 tgtggtgatg ttgagcgccg cgccctcgga ccgtcccaga agcacccagt gcccgcggcg
    13561 ccaccgctgg cgcagttcaa tcgtgctggg cccgatgtcg caccgcagta tgtcgatgtc
    13621 cccgaggccc accccggcga agagacggct gtcgacggga gctgggttga tgaccatgga
    13681 gctgagtcgc gcgaggattt catcgacatc tctcacgtcg ggcgccaagc gatcgatggc
    13741 gtgcggggtg ctcataccgt cgcctccaat cggagttcct ccatcagcgc cgcgatggcc
    13801 tgtgccccga cgaccggtcc gcggacgatc tccgccccgt tctcgccgtc gaacaggacg
    13861 gcagcggggt atgtcgtgat attcacctcg gccgcgagac cgccgtcgtg atcatggaac
    13921 atccgagcgc cgagtgcggg gaacgagctg atcgcgcgtc cggcctcgac gtcagtgatg
    13981 atgcggagac gcgttccgga tgcttcaaac gcggggatga cctcgaggat gctgtccgcc
    14041 agagcgtggc acgcagcgca gctggtgctc acgaacagaa gcagggatcg ttcttcgccg
    14101 tgccaggtgc gcgcgtcgac agcggtaccg aagagatcat gaagaaggtg ccgactgatc
    14161 ttcgagtctg gtcgggccgg tgaagcgatt ccgggggcgg gcttgagcgt cgacatgccg
    14221 atgaatgcgg ccagggctcc caggatgaac agccccgcct ggaacacggg gagctgcgac
    14281 ccgaggcctg ggatgatggc cagcagaggc ccgcgcgcgg atcccagcgc caccatgacc
    14341 gacaggatga gaagtgccgc gttgcgcacc acagtcgcag cactcaacgg cgtgcggtcg
    14401 agtcgcccga agcaattgca cgggcccgat acacccttgg ccagcgcgcg caggacgacg
    14461 gcgaagaaga ccgcgaacag cgcgacgcag gtgccgccga cgacgatgga cgctggagcc
    14521 ggcacgagca gcagggaaac agccagcgcc acctcgagca acggcacgcc gatgatgaag
    14581 atcggcgtgc gcagcacctg cggcatcgcg agtgtctcga agccggcgcg cgtcgtcgtc
    14641 ggtgacgcga gtttgagcgc gccactcgcc gacagcacgc cgatcaggca cgcgagcgcc
    14701 agcgcataca gtgaatcgct catcgtccac tcccccgaat gacgatcaaa gcgagacgtc
    14761 cctgaaagga cggtccccga cgcccctctt caggacgtcc cccatttttg ttccgatgtc
    14821 cccctgacac cggaaggcga gtctaaccgg tggtcgggtg aatccccgac caccggtcga
    14881 ctgatgctgt gccggatcga ctcagctggt ctgcttctgg caggtggcga cggttgcgaa
    14941 tcccagcgtg acgccggtga tctgcatccc tgcagcgttg ttggccgggt tgatgtcctc
    15001 accgagatag gcgaggtacc aggtctgcgc ggcgaagatg cccacgcccg tgaacttgac
    15061 gtccaggggc gtgctggggg tgatgtcgcg cggggtgcgg aacgtccagg cgttcgagcc
    15121 ggttcccaga atgtcgagcg ctgagcccac cgagccgtcg acgttccatg atccgggcgc
    15181 aagcgttgcg acgctcgagc cgtacatgat gaacgtcgtg ccctgtggaa tggtgccacc
    15241 gaccacgccg atgcggaacg tcgggatcga cccgaggacg agccaatcgc acttctggat
    15301 gctgacctgg atgtcgtact cgaccttggt gaccggcgga acgttgtcgt cgccggagtc
    15361 gaagcagatg aacgcgccga gcaggccgcc cacgcccaca ccgcctgcgg tcatgatcgc
    15421 aatgttgtca tcctgcacgg cgtcgggcgt gatgatgcga gaggtgtaga gcccgacgat
    15481 gttcaccgag aagcccttga gaccgatggt gaagctctcg ccggcgacgt agtcgcgggt
    15541 gagctcgaag gtcgcgacgt tgccgccgat gttgagcagg ctgatgccgt tcgccgcacc
    15601 gagctcgaag ttgcccgcgt tgatcgcggc cagaccgccg aaagtgatct ggatctgcgt
    15661 gcccttcttg atgatcccct cagtcaccgt gtagcggaat cccgggaagt tgctgcccgc
    15721 gatgccgagg gtgatcagct cgcagtcgac ggtctcgacc ttgaggttgt tctgggtcga
    15781 cgcagcgaat gcgggtgctg cagtgctcat ggcgatgacg ggcacagacc aggctgcgcc
    15841 cttgacgatc gtgcgccgcg agaattcctt cgagttcggg atggagttcc ccatgctttc
    15901 tccttcacat ttctagacga acgtctcctg tctagcccgc agcttctggt ccgggcgaac
    15961 cggggcgtct tttctggcga ttgctggcgc gttaagtgcc ggaacccgac acgatgtcgg
    16021 gtgggaggcc gaagaccgtt aggttggtgc cgtgaatccc gtgaccatcg ccgccgccga
    16081 tcagatcatc gccatcgact tcgacgggtc ggtgagcccg gaagctgctc gtatcgttgc
    16141 tgaaaccttc tctcatctgg agttctccgg tgatccgaag catcggatcg tggcgtcgat
    16201 gagtgcagag gccatggttc cctccggcgg cgcgttcgcg cgcgggacca cgacgccgaa
    16261 gctgatccgc agcatcgcgg acgcgctgga cgggctgatc atggcggcat tcgtgccgga
    16321 cacggcccgg gggagagtgg acgagctgcc acagcgggtg gcgctgaatg cgaccgcgtt
    16381 gaacacgccg gagggcgccg tcgtgatctt cggcggacac tcgcgccagc gtgcgatcac
    16441 ggccatgaga ggcaccgcgt cgttgatcgg cggcgacgtg agcgtgatcg accggaacga
    16501 tcggaacgtg agtggtgtgg caggggtgat caacatcggc ggtgccacga acgacacggc
    16561 ccgctgggtg agtccggatg ccgcaggcgt caccgtgcac ggggggccgt cgcaggtcag
    16621 ggcgttcgtg ttcctttcgg aggcggtcgg cgaggaagcc ggatggttcc ccgtgagcac
    16681 acccgaggcc gtagcgatgg cctcggggtc cgtgccatcg tcatcggtgg tcacgaggcc
    16741 gctgctcgga ctggcgggca tcttcgagat ggcgcccgct gctgtcgtca tccgctaccg
    16801 cgaagaatca cagatcccca cgctgatcga tgaagtcctg acggcgccgg gggacaagca
    16861 tcaggtgcag gcggcggacg tgcgcgctgt tgaggtgggt acgacgccgc tgctcgggga
    16921 tgtccggcga gcagctatgc gagacgcgat cactgatggg cgccacatgg ccttctgcac
    16981 ctcgcagtac tcggtgatcg cggtgggagg catcgcaccg atgctgtggg acttcgctga
    17041 ggggtggatc agcgtcgatg cactcagcac acgcctgatc gaggcgatcg gcgaaccaga
    17101 cggcgacgcg cgtgagcacg tcgctgccgc ggttgaagcg ctagtcattc agggcgtgct
    17161 ggtgcgagtg tgagcgggca cacgacgtgc cgatgctgat cgagtcggca tgtttcgcga
    17221 caggccgcag ccgacttcgc gacccgatgt gatcgatccg cctcacgtct ggtctcatga
    17281 tctgagcgtc tgctgatcgg cgcctgggga cagtcgatcg ctctcatccc agcgggagca
    17341 agcggaactc taccgaagag accgcgcggg cacatggcgg gcatccggca ccccctggag
    17401 cctgatgccc cgcactcccc agaccggtat ctcgaatccc gcgtgatgaa tcgcccccgg
    17461 gctccgtctc ttcggagcgt ctcgagcgac agggcgtcct cagtgtgctc cggacggttt
    17521 gacaacggcg gccgccgtac gtgcgaccac gacgatgtcg gtggtgatcg accagttctc
    17581 gacgtagtag aggtcgagcc gaatcgcgtc gtcccaggag aggtcggagc gtccgctgac
    17641 ctgccagagt ccggtgatac cgggcttcat gaacagccgg cggtgcgcgt cctcgtcgta
    17701 aagggcgacc tcggcctcac gctgcggacg tggtccgacg aggctcatgg taccgatcag
    17761 ggcgttcgcg aactgcggca gctcgtcgag cgagtaccgg cggatgaacc gtccgacggg
    17821 cgtgatgcgc gggtcgtcgt tgaccttgaa cagcggcttg tcagaggtgc cctgctgatc
    17881 gagcagcgac ttcagttggt catcggcgcc gacgaccatg gaacggaact tgaacatctt
    17941 gaacggcttg ccgtggaggc cgatccgctc ctgcgcatag aagatcggac ccctgctggt
    18001 gaacttcacc gcaagggcga cggcgatcat cacaggggag aacaggatga gcaggcctag
    18061 ggagccgacg atgtcgaagg tgcgcttggc gatgcgctgg cggccttcga agcgcgggta
    18121 ctccacgtgg atgagcggga ggccggcaac gggacgggtg tggatgcgag ggccggcgat
    18181 gtcggtgagt gccggagcga cgatcagact gatgcgcttc tgatcgagtg cccagccgaa
    18241 cttgcgggtg cgcttgggag tgatggcgtc cgacgtcgaa aggatcactg agtcgacttt
    18301 gtgggagtcg atgatgtcga ggacctcatc gaagtcgccg agcacgggca cgtcctccag
    18361 gagcggctcc tgggttccgt gagtggtcac ggcgccgatg atgcgtatgc cggactgcgg
    18421 ctctctgacg atcttgtcgg cgatgtgagt cgccttctcg cggtcgccga tcacgatcgc
    18481 gcgctgaagg aagtgaccgg cattccgctg atgacgcagc cagtttcgcc acagccatcg
    18541 agtgagtatc agcagtagca agcccgacgg cagcgcgatc agcaggtaga accggccgaa
    18601 ctggatttgg aacagaagtg ccaggatcgc gatcccgccg aagactctca tggtcgcgtc
    18661 gacgatgcgt cgatactcgg tcgagccgcc gtcgagcacg gacggctcgc gcgtgccgaa
    18721 catctggagc gcgaccagcc atccgagtac gaacagaagt gaggtgacga cgtagccgat
    18781 ctcgaaatcg aaggtgcgat cgccgatcgt cagcggttcg ttgttgaaac cgaaccgcag
    18841 aaggtgcgct ccgaacactg caagaatcac aaccgcggtg tcggtcatcg caagccgggt
    18901 tccgtaccgg cgctgctgtc gctccacgcg tgtcgtcgcg gatgccgcgt gtgacatgac
    18961 gctcatactg tgtgctcact catcttcagc cgtccccaag gcacaacgcg tttccctcgc
    19021 gcgtcggtgg ttcgaagcgg cgaccccctc ggtcggcctc gcgatgacgc cgcgcaacgg
    19081 gtgcgctcgg gcatcgcgct cattctcgca cgtcggccgg tggcatgcat atcgcgcaac
    19141 agtacggtat gcggcgtcac ggctttcgag cctccatcaa ggtagggtgg gcccaactga
    19201 gggtcaggaa atggagagcg gtttgaccgc gtggatctcg atcggattgt cgtcgatcgc
    19261 tgcgattttg gccgtcttcg catgccgcgg cgtcagtttt cgagagtggc ggtcgctggc
    19321 gttctggatc cccccggtgg tcatcgtctg gccgtcgatc gcgctcattc tcggtgcgct
    19381 tccgatagcc tccggcgtgt tccgcggcga cgtggtggtg ttcgggttgc aggtgatgcc
    19441 ctcggtactg gtctgtctac tgctcatggc ccgtgcggtg gtcgagcgga agcgagtcgg
    19501 cggtgtttca gcgctgatcg tctccgctgt tgttctcgtc ggcggcgttt tctcggtggt
    19561 ggccggacag agtctgctgc acgcgctcgt tgccttggcg gtcgtcgccg gctgtgcgtt
    19621 cgtcaggaat cgtgaagctc gggccatcgc tgacgcgctc gtgatctccg tcgccggcat
    19681 gtgcgttctt ctcgcaagct acggcctgct gcttcccggt ctcgcggtgc gaacggattg
    19741 ccagcagaat acggcgaagt gcgatcttct cgggggcttc ctctccatcg gccaggagag
    19801 tggcagcaat gcattcggga tcacccttgc gatggtcgcg gcattcgcgg tctacagatt
    19861 gcggtggtcg cgagcggtga tgctgaccgc tgcggtctgc gcgacgatcc tcgcgagtgg
    19921 ggcacgcctg gctacagcct gcgccgcggc atcctgtgcg atcggcctgc ttgccaaggc
    19981 gcgaggcggt cggacctggg cggccgtggc gctaagtgca gcaactgttg cctctctgat
    20041 tttcgccgtg gttccctttc caccgcattc cttcacggat agggctatcc tctgggggcg
    20101 tgctcgccag atgatcctcg acgcgccatg gttcgggcac ggactttcct tctgggtccg
    20161 tgacgctgca acgcagcccc ctccaccgcg aagttactcc ccgcacaacc tgtggctgga
    20221 tctcggggtg gcaggcggca tgatcgcagt cctggtgctc gtcgtcgccg ttctcctggg
    20281 actgtggctc tccgcaccgg aggcgcggac gggagcgctg ctgatcctca ccggcatcgt
    20341 cgtcgtcggc tgctttgaat cgacgttcat gccgttccgg atcaacccgg ttccggcagg
    20401 catggtcgtc ctgctcgcgt acctgagcgt caaccagaga accggccgtc ttcgcggcct
    20461 gttgcgacgg ctcgggcgag ccccagctgc atgacggact gcgcgacgac cggcgtcagg
    20521 atcagcgtcc agatcggcag cgcccatgcc gcgactgcgg cgtagagaag cgcagcaaca
    20581 gcaccgatcg ccaccgctct ggcggctgac gcagctctgt cgaaggcgaa aaggaccgct
    20641 tgatgggcct gattcacgcc catgagcgct ccactcacga cgaacccggc gatgaacggc
    20701 caggcctgcg tgtattcatc gccgagtagc caggcaccgg cgaacccggc gggaacagcg
    20761 aggatcagag ctgcgccgac gataccccat gtaacgtgcc gcatctgagt gaacaacgac
    20821 atccgctcgt gggggagtgc agtcgacagc gtgggcgtcg ctgcctgcag gagtgcctgc
    20881 gcgagcagat tcaccggtcc gccggcgcga gaccctgcac cgtagaggcc cgaggcggcg
    20941 cttcctccga tgagtgcgat gacaggcgtc tcgagctgcg acagagagga ggccaggttt
    21001 gcgcgccaat agctgaagct cgtcgtgatc agcttgatga gcgtggaccc gcgcggccat
    21061 ccacgtatgg tgaccgccac gttggctgct gtagcgatca acgcagcgcc gatcagccac
    21121 gacggctgtt cggtgctcac gaatgccgca gccgtcgtca gcaggagcag ccgctgcgcg
    21181 ataaggatca tcgtcgctcg gcccagctga tctgccccgg ccatcacggc ttggcagatg
    21241 tcggtgaccg catcgatgag gatgaacgag gcggccacga tgcaactcgc gacgggcagc
    21301 tcagcggagg tgccgatgag gaacaccaca accgcgagga gcgtgcccag tgcggccctg
    21361 aacgcgacca tcgtcgcccg tttcacggat gggtcatctc cctcggcacg cagacggagt
    21421 gcacggtgcg tgaaccctgc gccggtgagc gtggtgatga atgagccgac accgaacaag
    21481 gcagcgaaga caccatagtc gccgacgccc aaagcccgcg ctagcagcac gtaggtggcg
    21541 aactgcacca gcgcagcgct cagtgtgccg atgaatcgcc aggcgatcag gtgcaccata
    21601 gggttcacac gtgtctctcg gcggacggcg acaggagtcc gcgtatcccc tgtcgagcga
    21661 gctgctcggc ggcggcatcc agaactcgct cagtcgctcc cggttcggtc acgagcccga
    21721 gtcggcgcgc ggtcgtcgcg ctggtcgggc tcggtgcctt gccgtcatgg ctcaacacgg
    21781 cgtcgatgaa ccgctcgaca tccgcgacgt cggtgaggat cgcctcgggc gccacctcgg
    21841 cgatcggcgg attgttgaag cctcgggcgg tgtcgaacag atcgaaatcg gggaagagca
    21901 gcccgaaggg gcggcccgtc cgcagatagt cgacccacac gctcgaatag tccgagacca
    21961 gaccggcgct gagagcgagc agttggaaga agctcagtcc cgcctcgcgc agggagtcgg
    22021 aggtgacgac gtgcatgccc catgcttcag cgttgcggtc gtcgtcgagt tgatgggcct
    22081 tcgtcacaag aacgattccg cgcgcagcgc atgcctgcct gagagcctcc atctttctgg
    22141 ccgcgggctt gtccatctgc tcgactgccg atacctgacc cggccggtgc gtcggcagcc
    22201 acaggacgaa gggacagagc gggtcgaggc cgagcgctgc cagtcgctcc cggtccactt
    22261 cttcatcgaa ggcgtcatcc cgcgggtttc cggacaccac gacggacgcc gacggatccc
    22321 ggagctgcgt ggcgatgacc gcccgacccc agacggcgtt gttggttgtc gccacctgag
    22381 tgtcgtacag cagcggtgag agggggtcct gccctgactt ggggccatga ccgtgcccga
    22441 gaagcacatg gactcgcgag cgacccgggc ggggattgcc gaacaggccg tgcgtcatga
    22501 gaacaagccg tgcctgaagg aatcgcaggt atccacggag tgagttcttg cggatgatct
    22561 cgagacgtga agcatcccag tcgacgtagc ggagtgcatt gccgagttgt gcgcgcgact
    22621 cccgcgggtc ctcgcagagt agggcgacga ctgtggtcgc atcagctctt cgagtcagtg
    22681 ctccggcgat gcggagcgcg ttctcctcac cgtcgcgcca gccgtggacg acgatgatgt
    22741 cgcgggccgg cacagaggcg atgagtaagg gagtgagacc gcggatggcg cgcgacgtcg
    22801 aatgcggcgt catcttccct ccgcgtcctt cgtcaggggt cagctcctga cccctgacga
    22861 aggtaccacc gactgcgtca gggcctcact cgcgcaggtg ctctcgatgc tcccggtacc
    22921 acgccacggt ctgcgcgatc ccatcgccaa gactgatcga cggcgtccag ccgagctccg
    22981 ccaggcgtga gacgtcgagg agtttctgcg gtgtcccatc cggcttggcg gtgtcccact
    23041 cggtgacgcc gtggaatccc gtcgcttccg cgatggcatc ggcgatctcg ctgatcgtgg
    23101 cgtcgcgccc cgtgccgacg ttcacttgat cgtcgccgtc gtaatgctcc aggagatgga
    23161 gacatgcatc cgccatgtcg tcggcgtgca ggaattcgcg gcgaggagta cccgttcccc
    23221 agttggtcac gacaggagca cctgccgtcg ccgcctcgtc atatctgcga atcaaggcag
    23281 gcagcacatg ggagccacgg ggtgagaagt tgtcgttcgg gccatagaga ttcgtcggca
    23341 ttgccgagat ccagggcagc ccgtactggc ggcggaccgc ctggatgtgg agaatgcccg
    23401 tgatcttcgc gatggcatag gcgtcgttgg tcggctccag gtgccccgtc aggagggagt
    23461 cctcgcggat cggctgctcg gcgaacttcg ggtagatgca ggatgacccg aggaagagga
    23521 gacgctcgac cccggcctcc agtgcagcgt cgatgacgtt cgtctggatc tggatgttct
    23581 cgctcagaaa gtcgacgggg tacgtgctgt tcgcaaggat tccgccgacc ttcgccgcgg
    23641 ccagaacaac atatttcggg cgttgctctc ggaagaagtc gaatgctctc ccccgatcct
    23701 tcaggtcgag ttccttcgag ctgcgtccca cgaggtcgcc gaagcctgca gcttcgaacc
    23761 ggcgccagat tgccgagccg accaatccgc ggtgtccggc gacgtacgtg cgtgcgccgc
    23821 gatccaattc cttcggctcg aaggtcatcg tctcgtccac ccgtcgagtg tcacggtgtc
    23881 cacccacggg cggccctcat gtgcgagcgc ctggacgtcc gcctcgacca tcaggcgagc
    23941 caggtcgagc ccgtccacag tcgcctccca tccgagcgca tcacgggcct tggacgggtc
    24001 accgatcagc gcatcgacct ccgtgggccg gagatagcgc tcgtcgaaac gaacgtaatc
    24061 ctgccagttc atgcccacgt ggctgaatgc cgcgtcgagg aagtctcgaa tcgaataccc
    24121 gaccccggtg gcgagcacgt agtcgtctgg cgcgtctgcc tgcagcatcc tccacattcc
    24181 ctcgacatac tccgcggcat agccccaatc tcggatcgca tcgagattgc cgaggtacaa
    24241 gtcgctctgc tgcccggctt tgatgcgtgc gactgcgcgc gtgatcttac gtgtcacgaa
    24301 ggtctcgccc cgtcggggcg attcgtgatt gaacaggatt ccgttgacgg catacatcct
    24361 gtatgcctcg cggtagttct ttgtgatcca gaacgagtag agcttggcgg cggcgtacgg
    24421 ggaacgtggg tagaagggag tcgtctcgct ctgaggcggg ggcgtcgcgc cgtacagttc
    24481 cgaggacgat gcctggtaga agcgggtgga gatgccggag agtctcactg cttccagcaa
    24541 tcgaacggtg cccgttcctg tggtgtcggc cgtgtgctcg ggctcatcaa aagacacccg
    24601 gacgtgagac tgagccgcca ggttatacac ctcgtcggga tccacctgct ggagaagcgt
    24661 cacaagacgg gaaccatcgc gaaggtcgcc gtagtgcaat ttgagccgcg cgccggactc
    24721 gtgcgggtcc acgtagaggt ggtctatgcg tgctgtattg aaggtggatg ctcggcggat
    24781 gatcccgtgc acctcgtagc ctttggcgag caacaactcg gcgagatacg aaccgtcctg
    24841 gccggtgatt ccggtgacga gtgcgcgttt catgtggagc ctccttcgga cgcgtctgtg
    24901 atgagttacg tcagggtatc gagaacgacg atgacgcgcc gggcggctgg cgcccatgaa
    24961 cgctcaccac cgccgggaat acacgaccgc gagccgattt cgcgcgggac tagacggaac
    25021 taccactcga agggcgtagg aaagcagtca gagtctgcac aagaaagtga cgggtgacca
    25081 tcagcgtgca caggtaggtg actccgcagg cggcgcacac aacgatgagg gtgaccagcg
    25141 gaatcatccc tagccagctg gtgacccaca tcatcccgac ccccacgatg gcgagcggta
    25201 tcgagtagag caatggccga acgtgatcga gtatccccgt tcgcgcggga atgcctgcga
    25261 gagtcttgag cagcacgacg ttggtagcga gcgtcacaac acccatgatc accgcagccc
    25321 aggcagcccc gaatactccg ccccaaatcg ccccgaaaat gacaacggcg atgtgaacgc
    25381 tggacttgat cacgttcact atcaagccga cgcccattcg gttgagtgcc atgagtatgg
    25441 cgcccatcgg atttcccatg gctcgggtga ggccgacgcc cgacagaacc tggatcagtg
    25501 cagctaccca gagccaattc ggcccgaaga atacctcggt gaagggcaac gcaacgatcg
    25561 caagtgtgac caggagcggg gcattcacga tcgtcacggt ttcgatgacc cgacgtatcg
    25621 ctcgcgcctg tcgtgagcgg tcagattgga tttgagacat tgcggggaag gctactctgg
    25681 tgacaacccc attcagtcgc gccggcgtat taacggcgta ggtgaatgcg agattgtagc
    25741 cgccaagagc agtcggtgag accagccggc ctactgcgaa gctgccaatg ttcgcagcga
    25801 agaagctaac gatcatgtcg aggctggtga tgaggccgaa agacaggaat cgagtcgtct
    25861 cgacgagtct gaaatggaag cggggaaaga actcgcgacg cgaagcatag acaaacaaca
    25921 acgctttgag gctgaccgag aaggcatacg ctgccgcgac ggcaaccggg ccggcgccga
    25981 ggaagagcaa gacaagagtc gcggcgagga ttgtcgccgc accgaccatt tcgatgactg
    26041 cgactgagcg gaaggccatg cgacgttcca ggatccctct ggggacttgt ccatggggag
    26101 agaagatgaa tcccaaggcg atgacgtgca cgagagcagt cagttctggc atgcggaaga
    26161 accactcgaa cagcggcgcg gatacgtaca gggcaactgc gatcacgatt ccgaccagga
    26221 cattgagcca atagagcgaa gaaagttcgt ctcgagaggc attttgccgc tggatgatcg
    26281 agttggcgat gcccatgttc gcgatcgtgt ccgccagcgc caggatcacg agactcgccg
    26341 cgacaacacc tacttgtgtt ggttcgagca cccttgccac agaggcaagt tgcgccagtt
    26401 ggaccaccgc cgcgaacagg acggagatcg ctacccagaa agaacccgac agggttgccc
    26461 gttggaggtt tgtcatgagc cgagatcggc ttcggctgcg cgctcggtgg cgtccgggcg
    26521 gtcaagcgcc ttcgtcaggt ctcgtgaaag gtattcgata agactccgat cgtggtggcc
    26581 tctgaatacg cgatcgaccg ccaatgtgag cgcggccgct tcgggaagcg tcgtggagcc
    26641 gtcggggact ctgtatgggt gttgcctgag caacaattgc ataaaccggc gtcggacctc
    26701 ccaatcccaa tactctcttg atgaccggta gaatttgcgt ttcttgagaa acttcacaaa
    26761 gaagagcgaa tcaagtcggt tcaggaatat gcgcacaggg cttcgagctt cgccacgttc
    26821 gccaatcgat gagggggcgc caacttcgtc cggcatcgcg atccatgggt atgcgcccgc
    26881 aaaccgcacg gtgccctcga cgctgaccgg ccagtctgcg gggtttcgaa gcggcagacc
    26941 ggtatcgatg atgtgttgcg cagcagtgcg gttgatgaaa tacgctgtgg cggtcatggg
    27001 cgtcaggatc aactgctgaa cttcgacttc gccgagcatc agtttggatc ggggatcccc
    27061 gatcatttga cgtccataga gcatcaggat tgcaggctca tcaaaggccg ggagcgccgt
    27121 cagatgcgcg aagactgcag cgagtgcgtc tggcctcacg atacgggcgt cgtcctcgaa
    27181 gatgagggcc caatcggcgg cggaggacag aaaggtgaga tagctttcgc ggtgggccag
    27241 tgcacaacca atctctccgg agcgcagttc gcgtccgtac acaacacgag ccccctccgt
    27301 gctcgcatag ccggtcagag ggtgtccgcc gtactcgccc tcgacccccg gtgatctttg
    27361 gttccggata ccaatggctt gcagctgcac ttccagctcg gctccgcggt agttgcggtc
    27421 aagtccgatg atcagcgtga gaaccgacat tcgtagcgtc cccctgtagt cggattcgaa
    27481 tgacatttgg cccggaacga ggtgtttccg gacatctaca tgcgtgatgc gtttcgccaa
    27541 ttcctgggcg gccgttctgc atccgagtcg aaggcggggg cgacctgctc ggggcgcggg
    27601 ctaaggtttc ctatcgcaaa caagcacagc cacgatatcc atgccacccc atcgaactcg
    27661 ctagggcgga tcggcacttc tgcgatcccc agtgcaagta tcgtcatgac cgccatcatg
    27721 gggagaatcg ctccggcctt ggcatggacc aaggctgcgt gtatcgcagg gatgaggatc
    27781 agcgccaatg cgatgagggc gacgatgcct ccgatcagag cgaatgtgat cagcatgttg
    27841 tgaccgtgcc agtacgcgcc gttaacgatc cgagacacct caccttcgag cccgtacacc
    27901 ccagggccgt acccccaaaa cggccgttcc gggaacagat cgaaggaagc tcgccacacc
    27961 gcgccgcggc tggtgaatgc ggagtcgtcg gagcgcaacg gcacgataat cgagacgacg
    28021 acaacgattc cacttgccag gtagccgaca atcggccggc tcttcgacgg gatccatcgc
    28081 aagacaagcc cgacgaccag tgctatgccg gtcgcaataa cggctgtgcg gctcgaggcc
    28141 atcaacaagg tgagtgcaac agatgcgaag cccacgactc tgagcaggcg gcgcttgaac
    28201 aacagcgtga acggcagcgc gatcgccatt gacatgccca gtgggttcat ctgcggaaag
    28261 aatccggcaa gcacggcgtg gccgatgagt gccttgctgt cctcggtctc gtcagtccaa
    28321 gcgatatcgg tggttgcggc catcaacagt gagacagctg caatagcaaa gccgagccag
    28381 cccaggacga cgaggtcttc gggctgagtg tcgatgctcc agacagcgac tgcgatcacc
    28441 gctaccaacg tgacaaatgt gattccagaa ctgaccgact gggtgttgac aacgtgagtt
    28501 agccacagaa gccccactgc agagagtgtc agaataacgc ggtgcgggga gcgtcgtgaa
    28561 agcctggaga agttggcaag caggataatt ccagccacag cccatacgag gagtggcgca
    28621 gcattcctca aagtggtgcc gagtggcgtc aaggcaaccg aagccgtcgt tagtgccgtg
    28681 ggtgggatcc gtcctgactg tacgagcgac accgcccaac cgagcgtggt gtagcaaagc
    28741 aaagcaatcg cgagagccgc cagttggata cgacggcgac gcccattcgc gaggatggcg
    28801 gcatccgaat ggagacctga gtcgctgacg tgtcgatcgg ctgatccagg tagcgcgcgt
    28861 gcgtcgaagc ttgtactcat ccgaaagtcc ccgtcttctc ggtggcaacg gcaaccgggt
    28921 taggtcgctg ccttgacaga gaacggccgt gcgttacgca gtgattctag ctcactgctc
    28981 ggagagcgat ctcaatcagg tcatcgaagg aggttgcaac gctctcccaa gtgagctgtt
    29041 cggcacggtt ccaaccacaa gcgcgtaggc gagtcaagtc atccggcgtg ttgactcgtt
    29101 cgattgcatc cgcgaagaca acggggacct cgcgctggtc ctcagggatc cgaacggcga
    29161 cgccaccatc ttggacgacg tctggcaacc cgccgcgatc agacaccacg ggcacacatc
    29221 ccttcgccat cgcctctagc gcgaccatcc cgaacggctc ctcccaactg cagggaagta
    29281 ggaagatgtc gttggcggcg aacaggtcga agacctcgtc gtgcgggcga ggcccgagaa
    29341 acctagccac tgtgtcgcct tcgcgccgat tcagggcgct caccgctgcc tcgacctgcg
    29401 acaggtattg ggtaggccca ttctccgagc cggtgcctgt tcctccgacc aggtcgaggt
    29461 tcgccttgat tccccggcgc cgcagttcgc cacagatggc cacagcctcg agtggtccct
    29521 tctctggaat gatgcgtccg gcgtagatca accgaagcgt agccgaaggc tgcctctcaa
    29581 ctcgtcggtc tatctgtgaa cgtgagacgc cattagccac gacggcggat gggggcagag
    29641 cagggaatga ttcgactgca cggtccctga cgaagtcgct gcagaatgcg acatagtcga
    29701 tgtgggaaaa gacttccgcg gactcgtcca tggtgacgta ggttcgaagg tcattatgca
    29761 tatgcagaat caccgcgccg gtgaagccgg cttcgcgaag ctctcttgcg tagtgcgggc
    29821 gattgtgtat gtgaatgagt ttcgcttcgc ggatccggcg gtaggcggac cggacccaga
    29881 ggcggtccca gagcaggcgg ttgtatacga cttggaagga ttttccgatt cgaggtgcca
    29941 acaatctgga cgtgctgctg agcacacgct cgtaagcgtc atatagccgg acattggcaa
    30001 tcggaactgg agccgcaaaa ctctcggtcg cccgtggact tgcaacgaga gtgctgtgct
    30061 tcgagcgtga atacgtctcg cgtacccagg aagcgatcgc accgccagtc gagtcgaacc
    30121 gctcggcgcc tggcatcagc gccgcgacat cagtaatcga gcccacctgg gccatgtctc
    30181 ttctgctcat gcgatgccct tcccggtcaa caccttcagc ggaaggacca ggaccgaggg
    30241 cgctgttctc acaccgggac gcccccacgt gtaagccgac cgcagaacgc ggagcgccgc
    30301 tcctggtcga cgatgccgac atgcctggaa atatgtgacg gtgacgagga agtcgccgcg
    30361 aactcgcctg tcgacgtgag cgagggcatc gtgggcccac tcgagggagc gctccggagc
    30421 gatccctctc gagaccgagc ccccgccttc gccgagctga gtgagcgccg cgtccacctg
    30481 tgtcactcgc agcgccgttg cgtcccggtc gacgcgaatg agccaatcga tgtcctggtg
    30541 gaagcggaga ctctcgtcga aggggatctc gatggcgagg gacctcggga acaggagggt
    30601 cgaggtgtgc atcgcaccct gaccagcctt cacccggtcc tttcggaaca ggtagctggg
    30661 gaggggttcg ccgggggcca gccgacgttc gggccatgcg cgaccactgg gttcgagaac
    30721 gagcgtcgaa ctgatccacg gaacgtcatg tgcgcctgca gactgcacgg cgaggatctg
    30781 acgagccagc ttgtcaggca tccattcgtc gtcatcgtcg agaagtgcga tcagctcgcc
    30841 acgagcagct cggattcccg ccatgcgcgc gacgttccct ccggcgcgcg gaccggtgcg
    30901 gatgacgatc actcgagaat cgtccgggag gtcgagctcg tggtcagtgt cggcggcgac
    30961 gatgacttcg aggagatgct cggtctggcg cagggccgat tcgacggcga gacgcagctc
    31021 gggcctgccg atggtgggga tcactgcgga gacggtccac tccgtagaag tcatggtgtg
    31081 ctcacctctc tcagaaccgc atctccatag tcgtcccagg aacgagcatg cctggagccg
    31141 gcgaggtcgg cgttctcgcg acgctcgtcc cacgccagca tctcgtgcgc ggtcagcgcg
    31201 tctcgaagcg acgcgacgtc tccggcccgg agaatcgtgt gcgccgggcc gagcagttcg
    31261 tgcgaacctg tttcgaaggt cgtgatcact ggcaggcccg cggcttcggc ctccgtgacc
    31321 acgagggcga agttgtcctc aatgctcggc agcacgaacg cgtcggattc cgcgtagagc
    31381 cgggcgagct gcgtggcaga cagcgtgccg agcagacgga cgttggaagg cagcgcgcgg
    31441 accagctgcc tgtcgaagac ggagcccaca aggacaagat cgagctggag gcccttcacc
    31501 gcttccagga ggaagccgac tcccttcctg aggcagatct gcccggtgaa gacgacgcgt
    31561 gggcgagccc gacgagtcgg tatctgctcg gttggaggcc ggaagactcc cgggtcgact
    31621 ccatacggga tctgaatgat cttcgaatcc tcgacgccgt tgctccgcat ctgacctgca
    31681 accacgcgcc cgggaaccag cacgtcgtcg gcgagatcga gctcggtctc gatccgatcg
    31741 acgaaccaat cgggatatgt ctcggccttc gcacgacgtg ctccatagaa ggcctcgagc
    31801 cgctcgttgc gcactcgtgg gtgggcgtcg atctcatgaa gcacgaggcg tcgatcgctg
    31861 tttcgcgtga aggtctcgag cgatgagccc ggcatcgaga taagcgtccg tgcgtcgccg
    31921 aagtcgacat gacgcgctgc ccgatcgtaa gcgaccttcc acgtcgcgtt accgagggtg
    31981 tgcgtgagtg gactgcggac gcgcccgccg agaactcgga tgaactcgga accgggagaa
    32041 agccgcacat cctcaggaag agcgccggtc gctacccgag ccgactgttt cgcgacgaac
    32101 ccgctgatcg ttttggaacg gggaaggacc ctggccgcgc ctctggcgag gctgagcaga
    32161 ggtgcctggt cgcggatcgc ccgccgggtg aggatcccgt ggcggctggc gcgctgtgcg
    32221 atggcacgga tcgcattctc gtcgaacgca ctggacactg ccacggagtg ttccggcatc
    32281 cggaactcgg tgtcgcggct catcggtccc catgcagatc gtcaagggac gccgagaccc
    32341 tctcggccga ccccgaccaa gagtatcggt cgacgttgtc cgccccggcc gagcgaaggc
    32401 gatcgcgaac ttccgcctcg gagtcgagga ttctcaggga cgagatcatc gccccctcgt
    32461 cacccggatc gaagaacagt gcggactccc cgagagcctc tcgcaagggc gggatatcag
    32521 cagccgcgac cggtgctccc gcagattgcg cctcaagagg cggcacgccg aatccctcgt
    32581 acaaggaggg gaagatgaac ccgcgggcgc ctgcatagag ctcctgcaac agctgatcgt
    32641 ctacgcgacc gaggagctcc acaccggggg tcgcacgagg agaaccatcc gatcgcgcca
    32701 tcgacgtcgg gcgtgcactt ccgacgagcc gcagtgccgt cgtgcttccc gactccgccc
    32761 gatatcgctc gaaggcatcc accatccgat cgatgttctt gtgcgggagg aatgaggcga
    32821 cggccaggaa ataggcgggt ctgctttcca ggtgcgggcg gcggaacgcg gatccggcgg
    32881 cgttcggcac gactctgatc ttctccaagt cgatgccgta cacgtccacg atctcccggc
    32941 gggagaactc gctcaccgtg atgacctgtc gagcgcggtg aagcgtcaac gacgaaagca
    33001 tccgatacgc gatgcggaat cgcttcgtgt acgtctgcgg gaaccggaca tatgtcacgt
    33061 catgatgcgt gacgacctga ttccggtaca gcaccgggcc ggtgttcatc aacgacagga
    33121 gcaggggtga accgtggtcg cggcgaagca cgcggggcag gtcccattgc tcccacctat
    33181 gaccgctcgc tgtcccgacc tgctcgacct cgcgtccgcc gagagccgtg tggcggagag
    33241 cgccgggcgg tgcgaacagc cgcacatcgt ctctagtcct cgcgagctcc gccgtgatct
    33301 cctcagcgaa gcgttggaca cctgtgaggt cctgagtgag aaaccggcag ttgatggcga
    33361 tcatgagacg aaccggtcga tgtgctcgcg gaatgcaggt tcgtcgaatc gtgcggtcgc
    33421 atctcgcggc tcgaacgccg gcagggaggc cgctcgtcgc acggcatccg caaggtccgt
    33481 ggcagccgtc gatgcggctg cgaccccgct cacaccgtcc acgaacgtct ccacctgccc
    33541 gcccactgga ccggtcacca ccggcgtgcc ggcggcctgc gactcgacgg ggacgatgcc
    33601 gaagtcctcc acgggaggga agacgaacac gctggcgagc tgcatgagcg cgtacagcag
    33661 cgcatctgag ggggagacga cgatccgcac gggcaccttc gcggtcgcgg cgatcgtgcg
    33721 caggcgctgt tcctccgggc cgcgacctgc gatcacgacg ggcagaccag tccgctcacc
    33781 ggcggcgatc acgaggtcca gccgcttgta ggggatgaac cgggaggcgc cgaggatgaa
    33841 cggggtcggc agctgctcca gcaggcgccg ctcatcggca tctgccacct gcgtgcgcca
    33901 gtcgctctgc gagaggatgc gctccaccgc gaccggcggg tggatcacgg tcgcctcgcg
    33961 gtcccaggcc tgggcgatcc tgtcgcgcac gaacgcactg ttcgccgcga tggcggtggc
    34021 ctcctgcgcg cgccggcgat cgatgcgctg gaacagcggc gacacggccc taacagccag
    34081 gctgttcccg cgctcatcga gctcgggatt ccagatgtac ctcgccggcg tgtgggcgta
    34141 gacgtacttc ggcacgtcac ggcccgcgag cgtcgcgtgg tgggcgaaca ggtgcgaact
    34201 caccagcatc cactcgtaat cgcgcgacgc cggtagccgt cgccaggcgc cgacggtgaa
    34261 cggcagcgcc agcgccttgt gccggcgcag cggcgtgcgc gccagccatg tctcgcgaac
    34321 cgagcgatcg acgaaacggt gcggagcgtc gtcccacaga cactggatgt cggcatcggg
    34381 aaaggcccgc gccatcgcct cgagcacgtt ctccgccccg cccgaggact cgatccactc
    34441 gtggacgaga aggcccgtca ctagggtcgt cccatcccgt aatacgacca tccggcggca
    34501 cgccacgcag cggcatccag gccgttgcgg ccgtcgacca ccacgcgtcc gtcggtgagc
    34561 gacccggcgt gctctggtga cagctcgcga cggtactcgt cccattcggt caccatcacc
    34621 acggccgcgg cgccgcggat cgtctcatcg cggtccgctt cgaacgtcag ctgcgggtgc
    34681 agtcggcgcg cgttctcgat ggcctgcgga tcggtcacga cgacatccgc acccagcccg
    34741 ttcagccgga cggccacgtc gagggcggga gagtcgcgca cgtcatcgct gtgcggcttg
    34801 aacgcggcac ccagcacggc gaccttcttg ccgtacacgc tgccgccgag cccgtcgacg
    34861 acgagctgaa ccgcgcgttc gcggcggcgc agattgatcg cgtcgacctc gcgcaggaag
    34921 ttcaccgact cgccccggcc gagctcctcg gctcgcgcgg cgaaagcgcg gatgtccttc
    34981 ggcaggcatc caccgccgaa tccgatgcct gcgccgagga acttgcgacc gatccgcgcg
    35041 tcgtggccga
//
LOCUS       DATMSX010000154         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_106464,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000154 DATMSX010000000
VERSION     DATMSX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106464"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="VN041_04710"
     CDS             <1..>1501
                     /locus_tag="VN041_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28380.1"
                     /translation="QAIAFDADAPAVVVVPLAGVREDAGVPIAVAAALGISEITPGRR
                     LNETVPRPDLMARVTAMLGERRTLLVLDNCEQVIDGAAQCAAELLAAVQTLRIVTTSR
                     TPLAVAAEQVLPLDPLTIDADPEAPAVRLFMERAHAARPGATLDLETVARLCRHLDGL
                     PLAIELAAARVRTMTPEQIELRLENRFALLTTGDRSAPERHRTLQAVIEWSWDLLAPE
                     AQRALALLSVLPGGFSASIAEAVLGDAQADDVLDTLVSHSLLGVIENAAGAEVRFRML
                     ETVREFGQQRLQDAGSEGMAWEAVIDWATAFAREQGEGGRLFPAGLTAAQHLAVVREH
                     DNLVHILRHLLTESRDADAVLLFAVLGQSWFIRGSFTEFIGYAPRMIEAAASSDPERI
                     LADRIVITLAMGVLGCLISGDPRATRGLALLRLHKARAGEHLAPAWQLLTEALDAAAR
                     GETSSAAGMRAGQTDPRAQLAADILMSQAEENDGEAESAMNAARSAWERA"
BASE COUNT          210 a          521 c          556 g          214 t
ORIGIN      
        1 caggcgatcg ccttcgacgc cgatgcgccc gcggtcgtcg tcgtccccct cgccggtgtc
       61 cgggaggacg ccggggtgcc catcgcggtc gctgccgcac tcgggatcag cgagatcaca
      121 cccggccggc ggctgaacga gacggtgccg agacccgacc tgatggcgcg cgtcacggcg
      181 atgctggggg agcggcgcac gctcctggtg ctggacaact gcgagcaggt catcgacggc
      241 gccgcgcagt gcgcagccga gctgctggcg gccgtgcaga cgctgcggat cgtcacgacg
      301 agccgtacgc cgctggcggt cgccgccgag caggtgctgc cgctcgatcc gctgacgatc
      361 gacgccgatc ccgaggctcc tgccgtccgg ctcttcatgg agagggcgca cgccgcccgc
      421 cccggtgcga ccctcgacct ggagacggtc gcacggctgt gccgtcacct cgacgggctg
      481 ccgctggcga tcgagctcgc tgccgcacgc gtgcgcacga tgacgcccga gcagatcgag
      541 ttgcggctgg agaaccgatt cgcgctgctg acgaccggcg acagatccgc gccggagcgg
      601 caccgcaccc tgcaagcggt gatcgagtgg agctgggacc tgctggcgcc tgaggcgcag
      661 cgcgcgctcg ctctgctctc ggtgctgccg ggcggattct ccgcatcgat cgccgaggcg
      721 gtgctcggcg atgcgcaggc agatgacgtg ctcgacacgc tggtctcgca ctccctcctc
      781 ggcgtgatcg agaacgccgc gggtgcggag gtgcgcttcc ggatgctcga gaccgtgcgc
      841 gagttcggtc agcagcgact gcaagacgcc ggaagcgagg ggatggcctg ggaggccgtg
      901 atcgactggg cgaccgcgtt cgcccgcgag cagggcgagg gaggtcggct gttccccgcg
      961 ggcctcaccg ctgcgcagca tctggccgtc gtccgggagc acgacaacct cgtccacatc
     1021 ctgcggcacc tgctgacgga gagccgtgat gcggacgccg tgctgctctt cgccgtgctc
     1081 ggccagtcct ggttcatccg gggctccttc acggagttca tcggatatgc gccgcgcatg
     1141 atcgaggcgg ccgcgtcgtc cgatcctgaa cggatcctcg cggacaggat cgtcatcacg
     1201 ctggcgatgg gcgtgctcgg atgcctgatc agcggcgacc cgcgggcgac cagaggcctg
     1261 gcactgctgc ggctgcacaa ggctcgtgcc ggagagcatc tcgcccccgc ctggcagctg
     1321 ctgaccgagg cgctcgacgc cgcggcgcgc ggagagacct cgagcgcggc ggggatgcgt
     1381 gcggggcaga ccgacccgcg tgcgcagctc gccgcggaca tcctcatgtc gcaggccgag
     1441 gagaacgacg gtgaggcgga gtccgcgatg aacgcggccc gcagtgcctg ggagcgggcc
     1501 c
//
LOCUS       DATMSX010000155         7042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_68429,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000155 DATMSX010000000
VERSION     DATMSX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68429"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VN041_04715"
     CDS             <1..774
                     /locus_tag="VN041_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HWU28381.1"
                     /translation="PVAVLGLFIVVVALYPFIEMWVTGDKREHHIADRPRNAATRTAI
                     GVAGMIFYSVLWAAASSDLMATHFMLTMEGVIHTLQALLFVGPVLGYFITKRICIALQ
                     KKDREIVLHGYESGRIVRLPGGEFIEVHQPVDVYDRWKLVDYETYQPLVVRPNAKGRI
                     PWHENLRSSLSRWFYEDRLQPLTQAQIEEADAHQQHALAHDAEIEAEEIAGAHTRAGV
                     TDESQIEAPDKTVGETPNTPSSVIAKDPAPRKKKSENGE"
     gene            854..1366
                     /locus_tag="VN041_04720"
     CDS             854..1366
                     /locus_tag="VN041_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28382.1"
                     /translation="MSLRIRPAVAEDAAGIEEIEEAADQLLVALFRATDWPAPDAAAE
                     RLALPGYLLLAEYEGTPAGFVHVLDLDGHAHLEQVSVLPRYARRGIGRFLVRAAMDEA
                     ARRGHDDMTLRTYADVPWNAPFYASCGFTETEPGSEALRSLVTVEEQLGLLEYGRRVQ
                     MTASLTGSVI"
     gene            1403..1819
                     /locus_tag="VN041_04725"
     CDS             1403..1819
                     /locus_tag="VN041_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU28383.1"
                     /translation="MDRTAYFEAKLAHETDASDLYAALKAGEQVTVVDVRSDEAWAQG
                     RVCGAVHMHYREIAERAPQEIPAGSEVVVYCWSPGCNAGAKGALEFAKLGYAVREMIG
                     GFEYWVREGYPVEDRDGVHRRPVDPLTGVPRVRTQA"
     gene            complement(1892..2314)
                     /locus_tag="VN041_04730"
     CDS             complement(1892..2314)
                     /locus_tag="VN041_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HWU28384.1"
                     /translation="MRSNIILWWIIGVFFLVVAGVYTGWSLLDEYHGKVEWVGTVALL
                     FTALMSAMIAVYLRKTYKAQGGELPEDVLTADIDDGDPELGEFSPWSWWPLVLAGSAG
                     VFIVGLAVGQFLLPIGLAIFVVAIVGWVYEYYRGHFAR"
     gene            complement(2318..4042)
                     /gene="ctaD"
                     /locus_tag="VN041_04735"
     CDS             complement(2318..4042)
                     /gene="ctaD"
                     /locus_tag="VN041_04735"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWU28385.1"
                     /translation="MTTTAEPNMLQGVRPTTIPPRQAALLSSTRVEQKGNIVVKWITS
                     TDHKTIGYMYLIASVLFFLLGGVMALIIRAELFAPGMQIVPTKEQYNQLFTMHGTIML
                     LMFATPLFAGFANAVLPLQLGAPDVAFPRLNAFAFWLFLFGSTIAVAGFLTPQGAAAF
                     GWFAYQPLASATFSPGAGGNLWMLGLGMSGFGTILGAVNFITTVITMRAPGMTMWRMP
                     IFSWNTLITSLLILMAFPVLAAAILAAGADRVLGSHVYDPANGGVLLWQHLFWFFGHP
                     EVYIIALPFFGIVSEIFPVFSRKPIFGYKTLVYATISIAALSVAVWAHHMYVTGGVLL
                     PFFSLMTMLIAVPTGVKIFNWIGTMWRGSITFETPMVFSLGFLVSFVFGGLTGVILAS
                     PPLDFHLSDSYFVVAHFHYVVFGTVVFAMFAGFYFWWPKWTGRMLNERLGYIHFWMLF
                     IGFHMTFLVQHWLGVDGMPRRYADYSTYDGWEWGNQVSTIGAIILGASMLPFFLNVWI
                     TARKAPKVTVDDPWGYGASLEWATSCPPPRHNFTSIPRIRSERPAFDLNHPEAAEHPV
                     AAAAGEAK"
     gene            complement(4046..4948)
                     /gene="coxB"
                     /locus_tag="VN041_04740"
     CDS             complement(4046..4948)
                     /gene="coxB"
                     /locus_tag="VN041_04740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWU28386.1"
                     /translation="MPSKRRLRWAALPLGVAVAVALAGCSAAQTHGFLPGFVEGAKSA
                     TNQTDRVGGLWVTSWVILLIVGIVTWGLMAWALVMYRRRAGQTGLPVQLRYNMPIEIL
                     YTVIPLILILGMFFFTAKDQAEIERPWAKPDVEVTAIAKQWAWDFQYDGEEKDNSDAV
                     WTMGVQADADANGDIDRAKLPTLVLPVDKKVTIDLQSRDVIHSFWIIDFLYKKDMYIG
                     KDNSWSFIPTRIGEYDGKCAELCGEYHSMMLFNVKVVSQADYDAYLQTLRDEGNTGDI
                     TDAYDRLGNLPGTGAPSTGEEEGK"
     gene            complement(5039..5401)
                     /gene="erpA"
                     /locus_tag="VN041_04745"
     CDS             complement(5039..5401)
                     /gene="erpA"
                     /locus_tag="VN041_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWU28387.1"
                     /translation="MSDITLTEETTAAHSVSLTDAAAQKVKSLLEQEGRDDLRLRVAV
                     QPGGCSGLIYQLYFDERFLEGDETVDFDGVEVIVDNMSVPYLEGASIDFKDTISEQGF
                     TIDNPNAAGSCACGDSFH"
     gene            complement(5500..6915)
                     /locus_tag="VN041_04750"
     CDS             complement(5500..6915)
                     /locus_tag="VN041_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWU28388.1"
                     /translation="MTSSSSSASPASESAVLEAVALGIPAALSDLGNLVRIPGIAWPA
                     FDQTQLERSAAAIAELARETGVFDEVRVLRAHVDDTEELGQPAVLATRAARNGKPTIL
                     LYAHHDVQPPGADELWETPPFEPTVRDGRLYGRGAADDKAGIMAHIASLRAVAEVLGD
                     DLELGVALFIEGEEEYGSRSFARFLRDNADALRADAIVVADSGNLDSETPGLTVSLRG
                     NARFTLRVRTLEHASHSGMFGGAVPDAMMATVTLLSTLWDADGAVAVEGMTARAGETP
                     DYSEATLRDETGLLPGVSPVGRDDILGRIWNKPSITITGIDATSVAQASNTLLPEVSV
                     VISARVAPGQDAQDAYAAIETHLRAHAPFGAELTFTDVDLGDGFLVDTSGWAVELTRG
                     AMAAGYGKDAVDLGVGGSIPFIADLVREFPGAQILVTGVEDPHARAHSPNESLHLETF
                     RNAVRTEALLLTRMNSRAAAS"
BASE COUNT         1223 a         2328 c         2419 g         1072 t
ORIGIN      
        1 ccggtcgcgg tcctgggtct gttcatcgtg gtcgtcgctc tctacccctt catcgagatg
       61 tgggtcaccg gcgacaagcg cgagcaccac atcgccgacc gcccgcgcaa cgccgccacc
      121 cgcaccgcga tcggcgtcgc aggcatgatc ttctactcgg tcctgtgggc tgcggcctcg
      181 tccgacctca tggcgacgca cttcatgctg accatggagg gcgtgatcca cacgctccag
      241 gcgctgctgt tcgtcggccc ggtgctcggg tacttcatca ccaagcgcat ctgcatcgcg
      301 ctgcagaaga aggaccgcga gatcgtcctg cacggctacg agtcaggccg tatcgtccgt
      361 ctccccggcg gtgagttcat cgaggtgcac cagccggtgg acgtgtacga ccgctggaag
      421 ctcgtggact acgagaccta ccagccgctg gtcgtgcgcc ccaacgccaa gggtcgcatc
      481 ccgtggcacg agaacctgcg ctcctccctg tcgcgctggt tctacgagga ccgcctgcag
      541 ccgctcaccc aggctcagat cgaggaggcc gacgcgcatc agcagcacgc tctcgcgcac
      601 gacgccgaga tcgaggccga ggagatcgcc ggtgcgcaca cccgtgcggg tgtcaccgac
      661 gagagccaga tcgaggcccc ggacaagacg gtcggcgaga cgccgaacac gccgagctcc
      721 gtgatcgcca aggatcctgc accgcgcaag aagaagtcgg agaacggcga gtaacaccgc
      781 cactcccccg ctgaggcccc cgtcccatgg acgggggcct cagtcgtgtc ggcgcctcgg
      841 tacgctggat gacatgtccc tccgcatccg tcccgctgtc gctgaggatg ccgccggcat
      901 cgaggagatc gaggaggccg ccgatcagct gctcgtcgcc ctcttccggg cgacggactg
      961 gccggcaccg gatgccgcgg ccgagcgtct cgcgctgccg ggctacctgc tcctcgccga
     1021 atacgagggc acgccggcgg gcttcgtgca cgtcctggac ctcgacggac atgcccacct
     1081 ggagcaggtg tcggtcctcc cccggtacgc gcgacgcggc atcggacgct tcctggtgcg
     1141 cgcggccatg gacgaagcgg cgcggcgcgg gcacgacgac atgacgctcc gcacctacgc
     1201 cgatgtgccc tggaacgccc cgttctacgc ctcgtgcggc ttcaccgaga ccgagcccgg
     1261 gagtgaggcg ctgcgcagtc tcgtgactgt ggaggagcaa ctgggtctgc tcgaatacgg
     1321 ccgccgcgtg cagatgacgg cgtcactcac aggctccgtc atctgagatc gccgaggcag
     1381 acatccgcgt agtctgagcc tcatggaccg caccgcctat ttcgaagcca agctcgccca
     1441 tgagaccgat gcgagcgacc tgtacgccgc gctgaaggcc ggagagcagg tgaccgtggt
     1501 cgacgtgcgc tccgacgagg catgggcgca gggacgcgtc tgcggcgccg ttcacatgca
     1561 ctaccgggag atcgcggagc gcgcaccaca ggagatcccc gccgggtcgg aggtcgtcgt
     1621 gtactgctgg agccccggat gcaacgccgg agccaagggc gcactcgagt tcgcgaagct
     1681 cggctacgcg gtccgcgaga tgatcggcgg attcgagtac tgggtgcggg agggctaccc
     1741 cgtcgaggac cgcgacggcg tgcatcgccg cccggtcgac ccgctcaccg gcgttccccg
     1801 cgtccgcaca caggcctgag caccacacga gcagaaacgc tgaggggcct cccggaaaag
     1861 tccgggaggc ccctcagcgt gtgatggagg atcagcgggc gaagtgaccg cggtagtact
     1921 cgtacaccca gcccacgatg gccaccacga agatcgcgag accgatcggg agaaggaact
     1981 gaccgaccgc cagcccgacg atgaagaccc cggcggagcc tgcgagcacc agcggccacc
     2041 aggaccacgg gctgaactcg ccgagctcgg ggtcaccgtc gtcgatgtcc gccgtcagga
     2101 cgtcctcggg cagctcgccg ccctgggcct tgtacgtctt gcgaaggtag acggcgatca
     2161 tggcgctcat gagagccgtg aacagcagcg cgacggttcc gacccactcc accttgccgt
     2221 ggtactcgtc gagcaggctc cagcccgtgt agacgcccgc gacgaccagg aagaagactc
     2281 cgatgatcca ccacaggatg atgttgctgc gcatgggtta cttcgcctct ccggctgcgg
     2341 cggcgaccgg gtgctcggct gcctcggggt ggttcaggtc gaaggcggga cgctcgctgc
     2401 ggatgcgcgg gatcgacgtg aagttgtggc gcggcggcgg gcaggacgtc gcccactcga
     2461 gcgatgcgcc gtagccccag gggtcgtcca cggtgacctt cggcgccttg cgcgcggtga
     2521 tccagacgtt caggaagaac ggcagcatcg aggcgcccag gatgatcgca ccgatggtgg
     2581 agacctggtt gccccactcc cagccgtcgt acgtggagta gtccgcgtaa cggcgcggca
     2641 tgccgtcgac gcccagccag tgctgcacga ggaaggtcat gtggaagccg atgaacagca
     2701 tccagaagtg gatgtagccg aggcgctcgt tcagcatccg gcccgtccac ttcggccacc
     2761 agaagtagaa gccggcgaac atggcgaaca cgacggtgcc gaacaccacg tagtggaagt
     2821 gcgcgacgac gaagtacgag tcgctgaggt ggaagtccag cggcggcgat gccaggatca
     2881 cgccggtgag tccaccgaag acgaaggaca cgaggaagcc cagcgagaac accatcggcg
     2941 tctcgaacgt gatcgagcct cgccacatcg tgccgatcca gttgaagatc ttcacgcccg
     3001 tcggcaccgc gatgagcatc gtcatcagcg agaagaacgg caggagcacg ccgccggtga
     3061 cgtacatgtg gtgcgcccac accgccacgg acagggcagc gatcgagatc gtcgcgtaga
     3121 ccagggtctt gtatccgaag atcggcttgc ggctgaacac cgggaagatc tccgagacga
     3181 tgccgaagaa cggcagcgcg atgatgtaca cctcggggtg gccgaagaac cagaacaggt
     3241 gctgccagag caggacgccg ccgttggcgg ggtcgtacac gtgggagccc agcacgcggt
     3301 cagcaccggc ggccaggatc gcggcggcca gcacggggaa ggccatcagg atcagcagac
     3361 tcgtgatcag ggtgttccac gagaagatcg gcatgcgcca catcgtcatg cccggggcgc
     3421 gcatcgtgat gacggtcgtg atgaagttca ccgcaccgag gatcgtgccg aaaccggaca
     3481 ttccgaggcc gagcatccac aggttgccgc ccgcgccggg cgagaacgtg gcactggcga
     3541 gcggctggta ggcgaaccag ccgaaggcgg ccgcaccctg cggggtgagg aagccggcga
     3601 ccgcgatcgt cgagccgaac aggaagagcc agaacgcgaa ggcgttcaga cgcgggaacg
     3661 cgacatcggg agcaccgagc tgcagcggca ggaccgcgtt ggcgaatccg gcgaacagcg
     3721 gcgtcgcgaa catcagcagc atgatcgtgc cgtgcatcgt gaacagctgg ttgtactgct
     3781 ccttggtcgg cacgatctgc atgccgggcg cgaacagctc ggcgcggatg atcagcgcca
     3841 tcacgccgcc gaggaggaag aacagcaccg aggcgatcag gtacatgtac ccgatggtct
     3901 tgtggtcggt ggaggtgatc cacttgacga cgatgttgcc cttctgctcc acgcgcgtgg
     3961 aggacagcag cgcggcctgg cgcggcggga tcgtcgtggg gcgcacgccc tgcagcatgt
     4021 tgggttcagc tgtcgtggtc atgacttact tcccttcctc ttcgcccgtg ctcggagcac
     4081 cggtgcccgg aagattgccg aggcggtcgt aggcgtccgt gatgtcgccc gtgttgccct
     4141 catcgcgaag ggtctgcagg tatgcgtcgt agtcggcctg cgacacgacc ttgacgttga
     4201 acagcatcat cgagtggtac tcgccgcaga gctcggcgca cttgccgtcg tactcgccga
     4261 tgcgcgtcgg gatgaacgac cagctgttgt ccttcccgat gtacatgtcc ttcttgtaga
     4321 ggaagtcgat gatccagaag gagtggatga cgtcgcgcga ctggaggtcg atcgtcacct
     4381 tcttgtcgac gggcaggacc agcgtcggca gcttggcgcg atcgatgtcg ccgttcgcgt
     4441 cggcatcggc ctggacgccc atggtccaga cggcgtcgga gttgtccttc tcctcaccgt
     4501 cgtactggaa gtcccacgcc cactgcttgg cgatcgcggt gacctccacg tcgggcttgg
     4561 cccagggccg ctcgatctcg gcctggtcct tcgccgtgaa gaagaacatg cccaggatca
     4621 ggatcagcgg gatcaccgtg tagaggatct cgatcggcat gttgtagcgc agctgaaccg
     4681 gcagtccggt ctggccggcg cggcggcggt acatgaccag cgcccaggcc atcagacccc
     4741 acgtcacgat gccgacgatc aggaggatga cccacgaggt cacccagagt ccgccgaccc
     4801 ggtcggtctg gttggtggcc gacttcgcac cctccacgaa tccgggaagg aatccgtggg
     4861 tttgagcggc agaacatcct gccagggcca cggcaaccgc tactcccaaa gggagtgcgg
     4921 cccaacgaag gcggcgtttc gagggcacga tgcacctttc agatctcgga caggagcgct
     4981 ctcaagtcta gagcaagctc acatgcgatt cacgccaacc acgcaggttg gcgaccgttc
     5041 agtggaagct gtcgccgcag gcgcaggagc ccgccgcgtt ggggttgtcg atggtgaacc
     5101 cctgctccga gatggtgtcc ttgaagtcga tcgaggcgcc ttcgaggtac gggacgctca
     5161 tgttgtcgac gatgacctcg acgccgtcga agtcgacggt ctcgtcgccc tcgaggaacc
     5221 gctcgtcgaa gtacagctgg tagatcaggc cagagcatcc gccgggctgg acggcgacgc
     5281 gcagacgcag gtcgtcgcgg ccctcctgct cgagcaggct cttgaccttc tgcgcggccg
     5341 cgtccgtcag cgagacgctg tgggcggcgg tggtctcttc ggtcagcgtg atgtcactca
     5401 tgtcactcct tgcagacgtg cagcacggac ggctgctcac gctcgatcct accgccggtg
     5461 cgtgcacgga gggcctgccc cgcgcacgcg ccggtgccgt caggatgccg cggcgcggct
     5521 gttcatgcgg gtcaggagca gcgcctcggt gcgcaccgcg ttgcggaacg tctccaggtg
     5581 cagcgactcg ttcgggctgt gcgcgcgagc gtgcgggtcc tcgacgcccg tgaccaggat
     5641 ctgcgcgccc gggaactccc ggacgaggtc ggcgatgaac gggatggagc cgcccacgcc
     5701 cagatcgacg gcgtccttgc cgtagccggc ggccatcgcc ccgcgggtca gctcgaccgc
     5761 ccagcccgag gtgtcgacca ggaagccgtc gccgagatcg acgtccgtga aggtcagctc
     5821 cgcaccgaag ggcgcgtgcg cgcgcagatg cgtctcgatc gccgcgtagg cgtcctgcgc
     5881 gtcctgtccg ggggcgacgc gggcgctgat gaccacgctc acctcgggca gcagcgtgtt
     5941 cgaggcctga gcgacgctgg tcgcgtcgat gccggtgatc gtgatcgagg gcttgttcca
     6001 gatccgtccg aggatgtcgt ctcgcccgac cggcgagacg ccggggagca ggcccgtctc
     6061 atcgcgcagc gtcgcctcgc tgtagtcggg ggtctccccc gcccgggcgg tcatcccctc
     6121 cacagccacg gcgccgtcgg catcccacag cgtggacagc agcgtgacgg tcgccatcat
     6181 ggcatccggc acggccccgc cgaacatgcc ggagtgcgag gcgtgctcga gcgtgcgcac
     6241 gcgcagggtg aaccgtgcgt tgccacgcag tgagaccgtc aggcccggcg tctcggagtc
     6301 caggttcccc gagtcggcca cgacgatggc gtccgcacgc agggcgtcgg cgttgtcgcg
     6361 caggaagcgg gcgaaggagc gggagccgta ctcctcctcg ccctcgatga acagcgcgac
     6421 gcccagctcc aggtcgtcac cgagcacctc ggccaccgcg cgcagcgaag cgatgtgcgc
     6481 catgatcccg gccttgtcgt ctgcggcgcc gcgcccgtac aggcgtccgt cgcgcacggt
     6541 cggctcgaac ggaggggtct cccagagctc gtccgctccg gggggctgca cgtcatggtg
     6601 ggcgtagagc aggatggtgg gcttgccgtt gcgtgcggcg cgggtggcca gcacggcggg
     6661 ctggccgagc tcctcggtgt cgtccacgtg agcgcgaagc acccgcacct catcgaagac
     6721 gccggtctcg cgcgcgagct cggcgatcgc cgccgcgctg cgctccagct gggtctggtc
     6781 gaaggccggc caggcgatgc cggggatgcg cacgaggttc ccgaggtcgg agagtgcggc
     6841 ggggatgccg agggctaccg cctcgaggac agcggattcg gatgccggtg aagccgacga
     6901 tgaggacgag gtcatgcgag taatcttaag gtgaacccga ttccgaactg aggtcgaacg
     6961 tgcccaacgc cacccccgcc gccaacgacg acgcacccga gacgcccgcc gtgggcaagg
     7021 gccgcgccac gccgacgcgc gc
//
LOCUS       DATMSX010000156         7835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_106509,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000156 DATMSX010000000
VERSION     DATMSX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106509"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VN041_04755"
     CDS             complement(<1..231)
                     /locus_tag="VN041_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28389.1"
                     /translation="MTRIVVIGAGPAGLAAAASAAQAGADVVIVDEGERVGGQFWRHH
                     PAFTDPRLQHQLGRFAQLEAALAQVRVLSSASV"
     gene            complement(228..473)
                     /locus_tag="VN041_04760"
     CDS             complement(228..473)
                     /locus_tag="VN041_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWU28390.1"
                     /translation="MRVTISFDGQTLDGQDGQSIGGILLANGQRTWRTAAGAERGIFC
                     GIGICQDCVVTVNGTEGVRACQRTACEGDVVEREARA"
     gene            complement(637..1797)
                     /locus_tag="VN041_04765"
     CDS             complement(637..1797)
                     /locus_tag="VN041_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28391.1"
                     /translation="MRIIVIGAGAVGAACALALTEAGAEVIVLDRTGVAGETSSRCEG
                     NILVSDKEPGAEALMAIEANRAWRELAARLDEDRVPGQPATEFEAKGGVVVAFDGGAS
                     ALERFAETQREIGIRADVLDAAALRAAEPHLSPEVALGVHYPDDAQVQPSLAAQAMLA
                     RARHLGAQLRIVEVTGGLVDGDRVRGVQTTEGRIDADAVVNCAGPWSGDAAALFGSVL
                     DIRPRRGTILVTAPMSQRVFHKVYDADYVGAVGSGDAALQTSTVVESTPGGTVLIGSS
                     RERVGFSSEGLLAPLAEIADKATRLFPFLRDTMLLRTYFGFRPYAPDHLPAIGADARI
                     AGLFHAAGHEGAGIGLAPTTGALIAHAVLGTPAPLNPTPFLPSRPTLQGAIA"
     gene            complement(1932..2642)
                     /locus_tag="VN041_04770"
     CDS             complement(1932..2642)
                     /locus_tag="VN041_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28392.1"
                     /translation="MSTLTVDAADVYYGRVHALRELSFEVREGEIVCLLGNNGAGKST
                     TMNMLSGLVRPKSGTVRWGDMDLTTAKPWDIVSAGLIHVPEGRRIFSTMTVHENLLLG
                     GYTVRDQKLIAQRVDQVYQLMPRLAERRKQQGGTLSGGEQQMVAIGRAIIGGPKLLLL
                     DEPSMGLAPLVVKQVMETIQAVNAQGATVLLVEQNARAALKIADRAYVVETGQTTMDG
                     PAAELAADARVIDAYLGG"
     gene            complement(2639..3580)
                     /locus_tag="VN041_04775"
     CDS             complement(2639..3580)
                     /locus_tag="VN041_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28393.1"
                     /translation="MSALLEVKDLRLQFGGVKAVDGLSFTVEAGEILAVIGPNGAGKT
                     SAFNCISGFYLPTSGSVVFDGRDIVRQAPSFWRSLRLVDLLQSFGFYRVLPSRVTKWG
                     MARTFQNLRLFRELSVLDNVKTAMHANIREGFWATLLHTPGYRKAERECAEQARGWLD
                     FVGFTGDENLYVTQLPYGEQRRVEIARALATGPKLLLLDEPAAGLNFNEKQALMQLIR
                     SIRDLGVAVVLIEHDMGLIMNLAERIVVLNYGKEIAVGSPEQIKNDPIVIEAYLGVED
                     EPLDETKLQTGTVDLSLLDDSLLDGSSQGKGKKGKRS"
     gene            complement(3577..4734)
                     /locus_tag="VN041_04780"
     CDS             complement(3577..4734)
                     /locus_tag="VN041_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU28394.1"
                     /translation="MSRSQTRADAALRAPSPLFDRPAAPAGILNSNKGFLRALGTLAV
                     VLLAVLPFINATPYLMSIATSALIYVMLAMGLNVVVGYAGLLDLGYIAFFAVGAYISG
                     IFTTVLGWPMWAALPMTIAACIVAGIIIGAPTLRLRSDYLAIVTLGFGEIIRITANNL
                     QITGGPSGIHGIPTWSFGDWSFSDGFTLFGLEFPQRVVFYYFVAAVVVLVGVVGAGRL
                     AKGKMGRAWKAVRDDEDAAEAMGINTYIAKITAYIIGAVWAGIAGQLMATHLSAISPN
                     SFQFLYSALILMSVVLGGMGSTPGVIIGALFVSLAPELLREFSEWRFLIFGVLLVVVM
                     LFRPAGLWPSTAILPWLKKLKPVPPPTAGFMAVPAASANEDLETVEGEETR"
     gene            complement(4736..5623)
                     /locus_tag="VN041_04785"
     CDS             complement(4736..5623)
                     /locus_tag="VN041_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWU28395.1"
                     /translation="MQLIWNGLIVGSFYALVALGYSMVYGIIKLLNFAHGDIYMLGAF
                     TGFATLSAFGISNETSIPLLLVIMLLSMVTTGLIGVGIERIAYRPLRKSPRLAVLITA
                     IGVSFTLEYAVRQIFGPNPEAFPVRLESNGFAFAGMRITAAQIVLVVIAAGLMLVLAH
                     IVERTRSGRAMRAIALDPQGAQLMGINVNRVIATVFFIGSALAGAAGVMAGAYYGSID
                     FLMGFIIGLKAFTAAVIGGIGNLYGAMLGGLLLGLLESFGSAWFGGEWRDVFSFAFLI
                     LFLTFKPTGLLGARVVERM"
     gene            complement(5694..6884)
                     /locus_tag="VN041_04790"
     CDS             complement(5694..6884)
                     /locus_tag="VN041_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU28396.1"
                     /translation="MHTRTRTPRIRRALSIAAAIGAIIITAGCSGGLGGGGGTGGSSD
                     GPIKIGMLAPFSGSESAFGNYMKNGAQLAIDEINAKGGVDGRKLELEVQDDACDATTS
                     VAAANKLVTDGVVASVGGYCSGATLPTLPIFNEAKIPMVIPAANSNELVKQKLPGVFL
                     INGTGTQQAAAAVKYAQKSGAKTVAAINDATAYSKDLADSFAQQAKDAGLTVDFNAVI
                     TPGENDYSAVATQLANAKPDLVYWTGYYQEGGLIVRQSTDAGFEGTFLVGDGSVDAKF
                     AQIAGKGYTEHVLGTFTQTPDMLKGADKWIADYKKLAGADPGPYSTQAYDAVNVVAEA
                     MTTAKGTDFDKVVKALEGLKDFKTLGGPLTFMPDHTRSGGGFVIVSIDPSSGQFVLKD
                     DLQG"
     gene            complement(6998..7663)
                     /locus_tag="VN041_04795"
     CDS             complement(6998..7663)
                     /locus_tag="VN041_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28397.1"
                     /translation="MTGFGALLSPAESLREQVEGQLASRIVSGEIPPGQVLTVPTLAG
                     DFGVSATPVREAMLNLARRGFLRPIRNRGFEVTEVSPDELRELTEVRMLLEAPPMRQV
                     AGTLDDATVTSLRETADRIVNAAKTGKFDEYLASDTEFHLRILQCTGNHKLVETVREL
                     RQQTRLVGLVRLAETDALISTSLEHAELVDLLVAGRGEEAEALMRRHIHHVGGVWSGH
                     DEE"
BASE COUNT         1232 a         2664 c         2746 g         1193 t
ORIGIN      
        1 gacgctggcc gacgacagca cgcgcacctg cgccagcgcc gcctcgagct gcgcgaatcg
       61 accgagctgg tgctgcagac gcggatcggt gaacgccgga tggtgccgcc agaactggcc
      121 gccgacccgc tcaccctcgt cgacgatcac cacgtcggcg ccggcctgag ccgcggatgc
      181 tgcggccgcg aggccggcgg gccccgctcc gatcacgacg atgcgagtca tgcccgcgcc
      241 tccctctcga cgacgtcgcc ctcgcaggcg gtgcgctggc acgcgcggac tccctcggtc
      301 ccgttgacgg tcacgacgca gtcctggcag atgccgatgc cgcagaagat gccgcgctcg
      361 gcgccggccg cggtgcgcca ggtgcgctgc ccgttcgcca gcaggattcc gccaatgctc
      421 tgcccgtcct ggccgtcgag cgtctgcccg tcgaagctga tcgtcacgcg catgcagcgc
      481 tcccctcgct ctggcgacgc ccaggtcgca ctcgttgccg gatggcgccg ccattcgctg
      541 ctccaggtcg cactggatgc cgcgtggcgg acctggaggc ggcatccatc gcgacctgga
      601 cagccaaaga ggagttgtga tcgccgacgc gcacgctcat gcgatggccc cctgcagggt
      661 cggacgggag gggaggaacg gcgtcgggtt gaggggcgcc ggcgtaccga ggacggcgtg
      721 ggcgatgagc gcgccggtgg tcggggccag gccgatgccc gcgccctcgt ggcccgcggc
      781 gtggaacagg cccgcgatgc gggcatccgc cccgatcgcg gggaggtgat ccggcgcgta
      841 cggccggaag ccgaagtagg tgcgcagcag catcgtgtcg cgcaggaacg ggaacaggcg
      901 ggtggccttg tcggcgatct cggcgagcgg tgccagcagc ccctcggacg agaatccgac
      961 gcgctcgcgg ctcgagccga tcagcaccgt gccgcccggg gtggactcga ccaccgtgga
     1021 ggtctgcagt gcggcgtccc ccgacccgac cgcgccgacg tagtcggcgt cgtacacctt
     1081 gtggaacacg cgctgcgaca tgggcgcggt cacgaggatc gtgccgcgac gcgggcggat
     1141 gtcgagcacc gagccgaaca gtgccgcggc atcacccgac cacggacccg cgcagttcac
     1201 cacggcatcc gcgtcgatgc ggccctccgt ggtctgcacg ccgcgcaccc gatcaccgtc
     1261 gacgaggccg ccggtgacct ccacgatcct cagctgcgcg ccgaggtgcc gtgcgcgggc
     1321 gagcatggcc tgcgcggcga gactcggctg cacctgcgcg tcgtccggat agtgcacgcc
     1381 gagcgcgacc tccggcgaga ggtgcggctc cgccgcacgc aatgcagctg cgtcgagcac
     1441 gtcggcgcgg atgccgatct cgcgctgcgt ctcggcgaag cgctccagag cggatgctcc
     1501 cccgtcgaac gccacgacca cgccgccctt ggcctcgaac tcggtggcgg gctgtccggg
     1561 gacgcgatcc tcgtcaagcc gtgcggccag ctcgcgccag gcgcggttcg cctcgatggc
     1621 catcagcgcc tcggcaccgg gctccttgtc ggagacgagg atgttgccct cgcaacggct
     1681 ggaggtctct cccgcgacgc ccgtgcgatc cagcacgatc acctcggctc ccgcctcggt
     1741 gagcgcgagc gcgcatgcgg ctccgacggc gcccgcgccg atcacgatga tccgcatccg
     1801 ttctccttct ccgtccggca ccgctgggat cccggcatcc gcgacagttg tgccgcgtga
     1861 catattactc tgcgcgaaga agggatgcac tgcccttcgc catcggctcc aggcagtgca
     1921 tccctcgcgc gtcagccgcc caggtaggcg tcgatgaccc gggcatcggc cgccagctcg
     1981 gcggccggcc catccatggt cgtctgcccg gtctccacga cgtaggcccg gtcggcgatc
     2041 ttcagcgcgg cacgggcgtt ctgctccacg agcagcaccg tcgcgccctg ggcgttgaca
     2101 gcctggatcg tctccatgac ctgcttcacc accaggggcg ccagccccat cgagggctcg
     2161 tccagcagca gcagcttcgg gccaccgatg atggcgcggc cgatggcgac catctgctgc
     2221 tcgcctccgg agagcgtgcc gccctgctgc ttgcgccgct cggcgagacg tggcatcagc
     2281 tgatacacct ggtccacgcg ctgcgcgatc agcttctgat cccgcaccgt gtagccgccg
     2341 agcagcaggt tctcgtgcac cgtcatggtc gagaagatgc gccgcccctc gggcacgtgg
     2401 atgagcccgg ccgacacgat gtcccacggc ttggcggtgg tgagatccat gtcgccccag
     2461 cgcaccgtgc ccgacttcgg gcgcaccagc cccgagagca tgttcatggt ggtggacttg
     2521 cccgcgccgt tgttgccgag caggcagacg atctcgccct cgcggacctc gaagctcagc
     2581 tcgcgcagcg cgtgcacccg cccgtagtag acgtcggcgg cgtcgacggt cagagtgctc
     2641 atgagcgctt ccccttcttg cccttgccct gcgacgagcc gtccagcagc gagtcgtcca
     2701 gcagcgacag atcgacggtg ccggtctgca gcttggtctc gtccagcggc tcgtcctcca
     2761 cgcccaggta cgcctcgatc acgatcggat cgttcttgat ctgctcgggg ctgcccacgg
     2821 cgatctcctt gccgtagttg agcaccacga tccgctcggc cagattcatg atcaggccca
     2881 tgtcgtgctc gatcagcacg accgcgacgc cgagatcccg gatgctgcgg atcagctgca
     2941 tcagcgcctg cttctcgttg aagttcaggc ccgcagccgg ctcgtccagc agcagcagct
     3001 tgggacccgt ggcgagtgca cgggcgatct cgacgcgacg ctgctcgcca tagggcagct
     3061 gcgtgacgta gaggttctca tcccccgtga agccgacgaa gtcgagccag ccgcgcgcct
     3121 gctcggcaca ctcccgctcg gccttccggt agcccggtgt gtgcagcagc gtggcccaga
     3181 agccctcgcg gatgttcgcg tgcatggcgg tcttgacgtt gtcgagcacg ctcagttcgc
     3241 ggaacagccg gaggttctgg aaggtgcgcg ccatgcccca cttggtcacc cgcgagggca
     3301 gcacgcggta gaagccgaag ctctgcagca ggtcgaccag gcgcaggctg cgccagaacg
     3361 acggcgcctg gcgcacgatg tccctgccgt cgaagacgac ggagcctgac gtgggcaggt
     3421 agaacccgga gatgcagttg aacgcgctgg tcttgccggc gccgttcggg ccgatcaccg
     3481 ccaggatctc ccccgcctcg acggtgaagc tcagtccgtc gacggccttg acgccgccga
     3541 actgcagacg caggtccttg acctcgagca gcgcgctcat cgggtctcct ccccttcgac
     3601 ggtctcgaga tcctcgttgg cggaggccgc gggaacggcc atgaaccccg ccgtgggcgg
     3661 cggcaccggc ttgagcttct tcagccacgg caggatcgcg gtcgacggcc acagtcccgc
     3721 ggggcggaac agcatgacga cgaccagcag cacaccgaag atcaggaagc gccactccga
     3781 gaactcgcgc agcagctcgg gcgccagcga cacgaagagc gcgccgatga tcacaccggg
     3841 ggtcgatccc atgccgccca gcaccaccga catcaggatc agcgccgagt acaggaactg
     3901 gaagctgttc gggctgatcg ccgagaggtg cgtggccatc agctgacccg cgatgccggc
     3961 ccagaccgcg ccgatgatgt aggcggtgat cttggcgatg taggtgttga tgcccatcgc
     4021 ctcggcggcg tcctcgtcat cgcgcacggc cttccaggcc cggcccatct tgcccttggc
     4081 caggcgcccg gcgccgacca cgccgaccag caccaccacc gcggcgacga agtagtagaa
     4141 caccacgcgc tgcgggaact cgaggccgaa cagggtgaac ccgtccgaga agctccagtc
     4201 gccgaacgac caggtcggga tgccgtggat gcccgacggg ccgccggtga tctgcaggtt
     4261 gttcgcggtg atgcggatga tctcgccgaa gccgagggtg acgatggcga ggtagtcgct
     4321 gcgcagccgc agggtcgggg cgccgatgat gatgccggcc acgatgcagg ccgcgatcgt
     4381 catcggcagc gccgcccaca tcggccagcc cagcacggtg gtgaagatgc cggagatgta
     4441 ggcgccgacc gcgaagaagg cgatgtagcc gaggtcgagc aggcccgcgt agccgaccac
     4501 gacgttcagg cccatcgcga gcatcacgta gatcagcgcg ctggtggcga tcgacatcag
     4561 gtacggggtc gcgttgatga acggcagcac cgcgagcagc acgacggcga gcgtgcccag
     4621 cgcccgcagg aaccccttgt tggagttgag gatgccggcc ggcgcagccg gacggtcgaa
     4681 cagcggagac ggcgcgcgga gcgcggcatc cgctcgtgtc tgggaacggg acatgtcaca
     4741 tcctctcgac gacgcgggcg ccgaggagcc cggtcggttt gaacgtgagg aacaggatga
     4801 ggaaggcgaa cgagaaaacg tctcgccact cgccgccgaa ccaggcggag ccgaacgact
     4861 ccagcagtcc cagcagcagg ccgccgagca tggccccgta gaggttgccg atgccgccga
     4921 tcaccgccgc ggtgaacgcc ttcaggccga tgatgaagcc catcaggaag tcgatcgagc
     4981 cgtagtaggc gccggccatg acgccggcgg ctccggcgag cgccgacccg atgaagaaca
     5041 cggtggcgat cacgcggttg acgttgatgc ccatcagctg ggcaccctgc gggtcgaggg
     5101 cgatcgcgcg catggcgcgt cccgagcggg tgcgctccac gatgtgcgcg aggacgagca
     5161 tcaggcccgc ggcgatcacg acgagcacga tctgcgcggc ggtgatgcgc atgccggcga
     5221 aggcgaagcc gttcgactcg agccgcacgg ggaaggcctc ggggttgggg ccgaagatct
     5281 gccgcaccgc gtactcgagc gtgaacgaga cgccgatcgc ggtgatcagc acggccaggc
     5341 gggggctctt gcgcagcggc cggtaggcga tccgctcgat gccgacgccg atcaggccgg
     5401 tggtcaccat cgagagcagc atgatcacca gcagcagcgg gatcgaggtc tcgttggaga
     5461 tgccgaaggc ggagagggtc gcgaagccgg tgaaggcgcc gagcatgtag atgtcgccat
     5521 gggcgaagtt cagcagcttg atgatcccgt agaccatgct gtagccgagg gcgacgaggg
     5581 cgtagaacga gccgacgatc aggccgttcc agatgagttg catcacgggg atgtccttcc
     5641 gagagggagg ccgaatcggg cgggacgacc tgcgccgtcc cgcccggatc ggatcagccc
     5701 tgcaggtcgt ccttgaggac gaactggccg gagctcgggt cgatggagac gatcacgaag
     5761 ccgccgccgg aacgggtgtg gtcgggcatg aacgtgagcg ggccgccgag ggtcttgaag
     5821 tccttcagcc cctcgagcgc cttgaccacc ttgtcgaagt cggtgccctt ggccgtcgtc
     5881 atggcctcgg ccacgacatt gacggcgtcg taggcctgcg tggagtacgg gccggggtcc
     5941 gcgcccgcga gcttcttgta gtcggcgatc cacttgtcgg cgcccttgag catgtcgggc
     6001 gtctgggtga aggtgccgag cacgtgctcg gtgtagccct tgcccgcgat ctgcgcgaac
     6061 ttcgcgtcga ccgagccgtc accgacgagg aaggtcccct cgaagccggc atccgtcgac
     6121 tggcggacga tgaggccgcc ctcctggtag tagccggtcc agtagacgag gtcgggcttc
     6181 gcgttcgcga gctgcgtcgc gacggcggag tagtcgttct caccgggggt gatcacggcg
     6241 ttgaagtcga cggtgaggcc ggcgtccttc gcctgctggg cgaacgagtc ggcgaggtcc
     6301 ttggagtagg cggtcgcgtc gttgatcgcg gcgacggtct tcgcgccgga cttctgcgcg
     6361 tacttcaccg ccgcggcagc ctgctgcgtg ccggtgccgt tgatgaggaa gacgccgggg
     6421 agcttctgct tgaccagctc gttcgagttg gcggcgggga tcaccatcgg gatcttggcc
     6481 tcgttgaaga tcggcagggt cggcagggtt gcgcccgagc agtagccgcc gaccgaggcg
     6541 acgacgccgt cggtgacgag cttgttggcc gcggccaccg aggtggtcgc atcgcacgcg
     6601 tcgtcctgga cctcgagctc gagcttgcgt ccgtcgacgc cgcccttcgc gttgatctcg
     6661 tcgatggcga gctgcgcgcc gttcttcatg tagttgccga aggccgactc actgcccgag
     6721 aagggggcga gcatgccgat cttgatcggg ccgtcactgc tgccgccggt gccgcctccg
     6781 ccgccgaggc cgcccgagca gcccgcggtg atgatgatgg cgccgatcgc tgcggcgatg
     6841 gacagtgcgc gtcggatgcg cggggtacgg gtacgggtat gcatgtgctg gtcctcctgg
     6901 atgcctcatt gcattgccat gtaatagcac gtgacacatt actcacgtgg gggcgtcagc
     6961 ggaaccctcc gagacctatc cgtgatgcac ggatgggtca ctcctcatcg tggccgctcc
     7021 agacgccgcc gacgtggtgg atgtgcctgc gcatgagcgc ttcggcctct tcgccgcgac
     7081 cggcgacgag caggtccacg agctcggcgt gctcgaggga ggtggagatc agggcatccg
     7141 tctcggccag ccgcaccagg cccaccagac gggtctgctg gcgcagctcg cgcacggtct
     7201 cgaccagctt gtggttgccg gtgcactgca ggatgcgcag gtggaactcg gtgtcggagg
     7261 cgagatactc gtcgaacttc ccggtcttcg ccgcgttcac gatgcgatcg gcggtctcgc
     7321 gcagactcgt cacggtggcg tcgtcgagcg tgccggcgac ctgccgcatg ggcggcgcct
     7381 ccagcagcat ccgcacctcg gtcagctccc gcagctcgtc gggcgagacc tcggtgacct
     7441 cgaagccgcg gttgcggatg ggacgcagga agccgcggcg ggcgagattc agcatcgcct
     7501 cgcgcacggg cgtcgcgctg acgccgaagt cgccggccag cgtgggcacg gtcagcacct
     7561 gccccggagg gatctcaccg gagacgatgc gcgacgcgag ctgcccctcg acctgctcgc
     7621 gcaggctctc agcgggtgag aggagggcgc cgaagccggt catcggtcac cggtcacaag
     7681 gtgagtcatg cgtcatatgc tactcacccg cggtctcacc aggcggaggc gacctcggcg
     7741 tgggtccgca ggatcccctc ggtggtgcct gccggcgcgc gcccgatgcc gcactcggtg
     7801 gcgacgccga agcgggcgac gacagcggat gccgc
//
LOCUS       DATMSX010000157         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_143917,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000157 DATMSX010000000
VERSION     DATMSX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143917"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1285
                     /locus_tag="VN041_04800"
     CDS             32..1285
                     /locus_tag="VN041_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWU28398.1"
                     /translation="MPVQAETPAPTAPPVPDDPTDPVRAQQYWLDGARIREAWNTTLG
                     DGVTIAVLDTGIGKAAGAFGDSVAGGVDESGVGSKDGRSPVGVINSDHGTLVASTAAA
                     PGNADGTGMIGVAPRAKLLSISLGFPGSSATKPFTEQVADGMKWAVDHGAKVINLSFT
                     TNTLDWDKSWDDAFLYAFEHDVVVIVAAGNRGSGTSIVGAPATIPGVLTVGGVDQTGT
                     ASVEASTQGITIGVMAPSEHLLGLTADGGVVSWDGTSGAAPIVAGVAALVRAAHPELD
                     AANVINRIIKTAIKVPEMTKTPDVQYGYGLLDANAAVTASVPAVKANPMGDLKEWIRL
                     YRRAASPADPTPDATATPVAVPPLPAADAPTEPGSPLLPSAETLRYGTLPLLALTVPG
                     TLIALGVTAAARRIRSERGRRTPTP"
     gene            1306..>2727
                     /locus_tag="VN041_04805"
     CDS             1306..>2727
                     /locus_tag="VN041_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28399.1"
                     /translation="MPQNSTAPRILIVGGGYAGFYTAWKLEKHLRKGEAEVTMVDPLP
                     YMTYQPFLPEVAAGSIEARHAVVAHRRHLKRTNVITAKVTGIDHANKVATITPPLGES
                     YEFAYDQIVVTAGAVSRTFPIPGIADNAIGLKTIEEAVAVRDKLMSNFDKAASIPAGP
                     ARDRLLTVVVVGGGFAGIEVFAELRSLASSLVAKYPQLSFDDTHFHLIEAMGRIMPEV
                     SLKTSEWVLKDLAKRGASVHLDTQVTGAVDGNVELSTGEVIPTDLIVWTAGVMANPTV
                     VRGGDLPVEERGRIRTRADLRVGTAEEAVEGAWAAGDVSAVPDLSGGGVGGYCVPNAQ
                     HAVRQAKVLAKNLVAVLRGERTKDYFHKNLGAVAGLGLYNGVFQSGKIALKGFVAWVA
                     HRGYHGLAMPTWERKWRVLWDWWNNLWLGRDLVNLQTVQNPRYVFEEFAARPRPAADA
                     APAAAAKPAAAAAKPAEEAAPAKK"
BASE COUNT          428 a          972 c          928 g          399 t
ORIGIN      
        1 ggtcgctgct gcagcggcgc cggtcacgtc cgtgccggtt caggcggaga cgccggcgcc
       61 cacagcaccg cccgtgcctg acgatccgac cgacccggtc cgtgcacagc agtactggct
      121 cgacggcgcc cgcatccggg aggcgtggaa cacgaccctc ggcgacggcg tgacgatcgc
      181 ggtgctcgac accggcatcg gcaaggcggc gggggccttc ggcgactccg tcgccggcgg
      241 tgtcgacgag tccggtgtcg gcagcaagga tggccgctcg cccgtgggcg tgatcaacag
      301 cgaccacgga acgctggtgg cgtcgaccgc cgcagcgccc gggaacgctg acggcaccgg
      361 gatgatcggc gttgctcccc gcgcgaagct gctctcgatc tcgctcgggt tccctggttc
      421 ctcggcgacg aagccgttca ccgagcaggt cgccgacggc atgaagtggg ctgtggatca
      481 cggcgcgaag gtcatcaacc tgtcattcac gacgaacacc ctggactggg acaagagctg
      541 ggacgacgcg ttcctgtacg ccttcgagca cgacgtggtc gtcatcgtcg ccgcggggaa
      601 ccgggggagc ggcacgtcga tcgtcggcgc acccgcgacg atccccggcg tgctcacggt
      661 cggcggcgtg gaccagaccg ggacggccag cgtggaggca tccacgcagg gcatcaccat
      721 cggcgtgatg gcgcccagcg agcacctgct cggcctgacc gccgacggcg gggtggtctc
      781 ctgggacggc accagcgggg cggcgccgat cgtcgcgggg gtcgcagctc tcgtgcgcgc
      841 cgcgcacccg gagctggacg cggccaacgt catcaaccgg atcatcaaga cggccatcaa
      901 ggtgcccgag atgaccaaga ccccggacgt gcagtacgga tacggccttc tggacgccaa
      961 cgccgcggtc acggcatccg tgccggcggt gaaggcgaac ccgatgggtg acctgaagga
     1021 gtggatccgg ctgtaccgcc gcgcggcctc gcccgccgac ccgacacccg acgcgaccgc
     1081 gacccccgtc gcggtgccgc ctctgcccgc cgctgatgcc ccgacggagc ccggttcgcc
     1141 cctgcttccg tccgccgaga cgctgcggta cggtaccctg ccactcctgg ccctcacagt
     1201 ccctggtact ctgatagcgc ttggcgtcac cgcagctgcc cggcgaatcc gatcggagcg
     1261 cggtagacgc actcccacac cctgacgacg aggagttgtc cccctgtgcc tcagaacagc
     1321 actgcacccc gcattctgat tgtcggtgga ggctacgcag gtttctacac cgcttggaag
     1381 ctcgagaagc accttcgcaa gggcgaggcg gaggtgacca tggtcgaccc gctgccgtac
     1441 atgacgtacc agcccttcct gcccgaggtc gcagccggct cgatcgaggc gcgtcacgcg
     1501 gtggtcgccc accgtcgcca cctcaagcgc accaacgtca tcaccgcgaa ggtgaccggt
     1561 atcgaccacg ccaacaaggt cgcaaccatc acgccgccgc tgggcgagtc ctacgagttc
     1621 gcgtacgacc agatcgtcgt gaccgcaggc gccgtctcgc gcaccttccc gatccccggc
     1681 atcgccgaca acgcgatcgg actcaagacc atcgaagagg ccgtcgccgt gcgcgacaag
     1741 ctgatgtcga acttcgacaa ggccgcctcg atccctgccg gcccggcgcg cgaccgtctg
     1801 ctgacggtcg tcgtggtcgg tggcggcttc gccggcatcg aggtgttcgc cgagctgcgc
     1861 tcgctggcat cctccctcgt cgcgaagtac ccgcagctgt cgttcgacga cacccacttc
     1921 cacctgatcg aggccatggg gcgcatcatg cccgaggtct cgctgaagac cagcgagtgg
     1981 gtgctgaagg acctcgccaa gcgcggtgcc agcgtgcacc tcgacacgca ggtcaccggc
     2041 gccgtcgacg gcaacgtcga gctgtccacg ggtgaggtca tcccgaccga cctgatcgtc
     2101 tggaccgccg gtgtcatggc caatcccacc gtcgtgcgcg gcggcgatct gccggtcgag
     2161 gagcgtggtc gcatccgcac ccgtgccgac ctgcgcgtcg gcaccgccga ggaggccgtc
     2221 gagggcgcct gggccgccgg tgacgtctcg gccgtccctg acctgtccgg cggcggcgtc
     2281 ggcggctact gcgttcccaa cgcccagcac gccgtgcgtc aggcgaaggt cctcgcgaag
     2341 aacctcgtgg ccgtgctgcg tggcgagcgg accaaggact acttccacaa gaacctcggt
     2401 gcggtcgcgg gtctcggcct gtacaacggc gtgttccagt ccggcaagat cgcgctgaag
     2461 ggcttcgtcg cctgggtcgc gcaccgcggc taccacggcc tggccatgcc gacgtgggag
     2521 cgcaagtggc gcgtgctgtg ggactggtgg aacaacctgt ggctgggtcg cgacctggtg
     2581 aacctgcaga ccgtgcagaa cccccgttac gtcttcgagg agttcgctgc gcgtccgcgt
     2641 cccgcggcgg atgctgcgcc agctgctgcg gcgaagccgg ctgctgccgc ggcgaagccg
     2701 gcagaggagg ccgcaccggc gaagaag
//
LOCUS       DATMSX010000158         8957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172760,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000158 DATMSX010000000
VERSION     DATMSX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172760"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VN041_04810"
     CDS             complement(<1..1728)
                     /locus_tag="VN041_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU28400.1"
                     /translation="MTQDAAVTTATKDATRQGAASASPELDDATRAALDADIALLQQG
                     SRTWSNVTVAQRVTLLRALRTSVAATIEDWADTAADSKGLRPGHPLRGEEWLSGPYSV
                     LGAIDAYADTLTKLSEGRSALDGVRIGRAPGGRTKVDAFPLTGIDRFLLSGFTGEVWL
                     EPGITPNTARESAGLAQLTPSASGGVGLVLGAGNITSIPVLDVMYELLAHNRVALLKV
                     NPTQDTLIPVYERALAPLIEPGFLRIVRGGPAVGAYLTGHRDLVHVHITGSAATFDAI
                     VWGSGATGKRRRRENRPQLKKPITAELGGVSPIIVVPGDWTDADFAYQAEHIATMRLQ
                     NCGHNCIAGQVVIMSSDWPQAEQFRTALRRAYAGAPERPIWYPGSPVRMQQASDAYPD
                     ALVLADRLLVEIGDGQDATALENTEYFAPVLGVVELPGTGQQFLDAAVEHANEKLQGT
                     LGGNLIIDPATEKALGTGFERAIAELRYGSIAINGWTAFGFITPTLTWGAFPGNTLDD
                     VGSGIGVVHNALLLDGVERSIVRGPFRPFPRSLPVLNGGGRFTILPTPPWFVSARTAA
                     QVSEGLTRFR"
     gene            complement(1802..2065)
                     /locus_tag="VN041_04815"
     CDS             complement(1802..2065)
                     /locus_tag="VN041_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWU28401.1"
                     /translation="MTTPASETITMFGAAWCGDCRRTKKQLDELGIEYTYIDLVADPA
                     AADVAKEISGRTNIPVVVYPDSSHHVEPSNADVEAKLRELSLI"
     gene            complement(2121..2900)
                     /locus_tag="VN041_04820"
     CDS             complement(2121..2900)
                     /locus_tag="VN041_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWU28402.1"
                     /translation="MTNNEQTFTRPVTTASGNPIEVRDLHKSFGKNEVLTGIDLTVAN
                     GEVVAVIGPSGSGKSTLLRCLNRLEEVTSGEVLILGENIATAKGKSLDAVRQRVGMVF
                     QHFNLFPHMTALENVALAPVELKMLSKAEARKRGIELLTRVGLAEKADARPASLSGGQ
                     KQRVAIARALAMSPGIMLFDEATSALDPEMVGEVLQVIRDLAASGMTMVLVTHEMGFA
                     REVADRVVFMADGKVVESGPPAEIFDSPKEPRTQDFLSKVL"
     gene            complement(2910..5207)
                     /locus_tag="VN041_04825"
     CDS             complement(2910..5207)
                     /locus_tag="VN041_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HWU28403.1"
                     /translation="MTLLRHPANWWRENRLPLLASLTTLLILLFTVGGAVGIANAATK
                     PASADDGVKGKTFTIATDTTWAPFEFQRDGELRGIDIDLIKAVAEDQGFDVKVDVLGF
                     DGALAAVTAGQADAVMAGMSITKERQKTFDFSNPYFDSGIQMAVAKSDASIKGYEDLK
                     GKTVSAKTGTEGYTFAENLGKQYGFTVTGFQDAADAYNDVTTGNSAAVFDDYPILAYG
                     IATGNVDLKTVTPQEKASSYGMGVKKGANEALRKAFNAGLKNLIADGTYQKILDDYLG
                     ENAPKAAAIGEGKAALGNDKLDPGKPGELPKNPAFATDTAQTHGEFTIATDTTFAPFI
                     YQLDGKNVGIDMDLMRAIAANQGFTVNVKTLGFDAALQAVKAGQADGVIAGMSITDER
                     KQVFDFSNPYYQSGVQMAVAKDSDITSYKDLKGKKVAVKTGTVGYDFAKKLGTEIGFT
                     VNAFQDSADMYNDVATGNSVAAFEDAPVLQFGIASGNVPLKIVTDAEPGSDYGFAVLK
                     GQNAELLKMFNAGLQNAKDTGAYQQIVDRYLAIDDASATADNSFLSLLKTSIGPLSQG
                     LLLTLLATALSILFAMALGILFGILKLSTNPFLRFLASAYVNIFRGTPVLVQAFFFYF
                     GVPAVTGQPLDALTAGVITLSLNAGAYITEIVRGGVQSVDPGQMEASRSLGLGWGASM
                     RRVVMPQAFKIMTPNLINQFIITLKDTSLLSVLGFAELTYQGRIVIASTFRSFEIWII
                     VGAMYFIVIWLLTVLSNWVDRKFNK"
     gene            5397..6596
                     /locus_tag="VN041_04830"
     CDS             5397..6596
                     /locus_tag="VN041_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU28404.1"
                     /translation="MSASVTVPAVTVIVPTYNERDNVAELVARTAAALEGFDAEILFV
                     DDSSDGTMDEIGRVAASAPIPVRGIHREHNTGGLGGAVVRGLSEAAHDVCIVMDGDLQ
                     HPPELLPTLLARFGTGDADVVAASRYIGGGDTAGLGTSVRLGVSKAATAVTKAMFLRR
                     LWRSTDPMTGFFLVDRSRLDVGTLRPEGFKILLEILVRGGSAGPLRIAEVPMEFGARR
                     HGTSKASLRQGAMFLAHLARLRFGKMGLFAMIGGIGAVANIGIMWALTHLGMDYVLAA
                     VIGAVVTIAGNFVLQELFVFRDERADASRLWVRFAASASFNGIEAALRIPVMALMVES
                     WHISAVVATAISLVVAFFARFLFHALVVYAPRRRRADSAEEPPVDTAAIRVLKAIDAE
                     AMRPGEL"
     gene            6642..7826
                     /locus_tag="VN041_04835"
     CDS             6642..7826
                     /locus_tag="VN041_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWU28405.1"
                     /translation="MTDYATGARFRLGRMLPRDPHEPHRVASSLELFFDLVFVISVST
                     ASSQLHHALSEGHAATGITSYLMIFFAIWWAWMNFTWFATSFDVDDWLYRVVTIVQMA
                     GVLVMAAGIAPVFDAHEPDFTIVVVAYVVMRVAMIVQWLRASRTNPALRRTALGYAIG
                     IGAVQVLWVGFLFIPNSIQLPVFIVLMLVEISVPIIAERQGSTPWHPHHITERYSLFT
                     LIVLGESLLASATAIIGAREEVDSLGPLIWIAAVTLISAAAMWWIYFWPPHHRAISTL
                     RSSLVYGYAHYFVFAAAAAFSAGVEVQIDELTGHTELGFVPASFTTSVPIAVFLLGIW
                     IVAIRDNADRVVNTVVPVGAVLVLLDAVLPVPVTLTAVILVAIVVVLVLRPPVRKEQS
                     AR"
     gene            7962..>8957
                     /locus_tag="VN041_04840"
     CDS             7962..>8957
                     /locus_tag="VN041_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWU28406.1"
                     /translation="MGWRMPHEGDPHERTWMTFPRAGTTLGMGDEHRESGYRAWADTA
                     LAVAEFEPVTLLVDPTETDRARRMLGTTVDIVEAPVDEFWLRDSGPTFVVDDERPGVL
                     GAVDWIFNGWGAPAWAEWQKAAQHARIVAGHVGAEIVSSLLVNEGGGIHVDGEGTVLL
                     TETVQLDPNRNPYADRARVEAELARTIGASKAIWLPRGLTRDYDEFGTNGHVDIVAAF
                     AGPGRVLLHRQDDAGHPDHAVTRELKAFLQDQTDAAGRPLEIIDVPAPATLRDDEGFV
                     DWSYINHLVVNDAVIACGFGEDAADARARDILEAAYPGRRAVTVDARAIFARGGGI"
BASE COUNT         1274 a         2992 c         3167 g         1524 t
ORIGIN      
        1 gcggaagcgc gtgaggccct cgctcacctg tgcggcggtg cgcgcggaca cgaaccacgg
       61 cggggtcggc aggatcgtga agcggccgcc gccgttcagg acggggagtg accgcgggaa
      121 cgggcggaac ggaccgcgca cgatcgagcg ctccacgccg tcgagcagca gcgcgttgtg
      181 caccacgccg atgccgctgc ccacgtcgtc gagggtgttg ccggggaacg cgccccaggt
      241 cagggtcggg gtgatgaacc cgaaggccgt ccagccgttg atcgcgatgg agccgtagcg
      301 cagctcggcg atggcccgtt cgaaaccggt gccgagcgcc ttctccgtgg cagggtcgat
      361 gatcaggttt ccgccgagcg tgccctgcag cttctcgttg gcgtgctcga ccgcggcatc
      421 caggaactgc tgcccggtgc ccggcagctc cacgacgccc agcacaggcg cgaagtactc
      481 ggtgttctcc agggccgtgg catcctgccc gtcgccgatc tcgaccagca ggcggtcggc
      541 cagcacgagg gcgtcggggt aggcgtcgct cgcctgctgc atgcgcacgg gggatccggg
      601 gtaccagatc gggcgctcgg gggcgccggc gtaggcgcgg cgcagcgcgg tgcggaactg
      661 ctcggcctgc ggccagtcgg acgacatgat cacgacctgc cctgcgatgc agttgtgccc
      721 gcagttctgc agccgcatgg tggcgatgtg ctcggcctga taggcgaagt cggcgtccgt
      781 ccagtcgccg gggacgacga tgatcggcga gacaccgccg agttctgcgg tgatcggctt
      841 cttcagctgc ggccggttct cacgccggcg gcgcttgccc gtggctcccg agccccagac
      901 gatcgcgtcg aaggtcgcgg ccgagccggt gatgtgcacg tgcaccagat cacggtgacc
      961 ggtgaggtag gcgcccacgg cggggccgcc gcgcacgatg cgcaagaagc cgggctcgat
     1021 cagcggtgcg agggcccgct cgtacacggg gatcagggtg tcctgcgtgg ggttgacctt
     1081 gagcagtgcg acccggttgt gcgcgagcag ctcgtacatc acgtcgagca cggggatcga
     1141 ggtgatgttg cctgcgccga gcaccagccc cacgccgccc gacgccgagg gcgtcagctg
     1201 cgcgagaccc gcggattcac gcgcggtgtt cggggtgatg cccggctcca gccacacctc
     1261 gccggtgaag cccgagagca ggaagcggtc gatgcccgtg agcgggaacg cgtcgacctt
     1321 cgtgcgtccg cccggtgcgc gaccgatgcg cacgccgtcc agggcgctgc gtccctcgga
     1381 gagcttggtg agggtgtcgg cgtaggcgtc gatcgcgccc agcaccgagt agggaccgct
     1441 cagccactcc tcgccgcgca gcggatgccc ggggcgcagc cccttggagt cggcggcggt
     1501 gtccgcccag tcctcgatcg tggcggccac gctcgtgcgc agtgcgcgca gcagcgtcac
     1561 gcgctgcgcg acggtgacgt tcgaccaggt gcgggagccc tgctgcagca gagcgatgtc
     1621 ggcgtcgagc gccgcgcggg tggcgtcgtc gagttccggg gaagcggatg ctgcgccctg
     1681 gcgggtggca tccttcgtcg ccgtggtcac ggccgcatcc tgagtcatcg tcgtctcctt
     1741 cgggatggaa cccagcctat gagttcgtgg gaccgggttc caccctcggg tgcacgcgga
     1801 atcagatcag cgagagctcg cgcagcttgg cctcgacgtc ggcgttcgac ggctcgacgt
     1861 ggtggctgga gtcggggtag acgaccacgg ggatgttcgt gcggcccgag atctccttgg
     1921 ccacgtcggc ggcagccggg tcggcgacga ggtcgatgta ggtgtactcg atgccgagct
     1981 cgtccagctg cttcttggtg cgacggcagt cgccgcacca ggcggcgccg aacatggtga
     2041 tggtctcgga tgcaggagtg gtcatacatc cagggtaagg ccgggggacc gggcgggcgc
     2101 cgggagcgcc cgcccggcgc tcagagcacc ttggacagga agtcctgcgt gcggggctcc
     2161 ttggggctgt cgaagatctc ggcgggcggg ccggactcga cgaccttgcc gtcggccatg
     2221 aatacgacgc ggtcggcgac ctcgcgggcg aagcccatct cgtgcgtgac cagcaccatc
     2281 gtcatgccgg atgctgcgag gtcgcggatc acctgcagca cctcgccgac catctcgggg
     2341 tccagggcgc tggtggcctc gtcgaacagc atgatgcccg ggctcatcgc cagcgcgcgg
     2401 gcgatggcca cgcgctgctt ctggccgccg gagagggatg ccggcctggc atccgccttc
     2461 tcggccagcc ccacgcgcgt gagcagctcg atgccgcgct tgcgcgcctc cgccttgctg
     2521 agcatcttca gctcgacggg tgccagcgcc acgttctcga gggcggtcat gtgcgggaac
     2581 aggttgaagt gctggaacac catgccgacg cgctgacgca cggcatccag actcttcccc
     2641 ttcgcggtgg cgatgttctc gccgaggatc agcacctcgc cactggtgac ctcctccagg
     2701 cggttcaggc agcgcagcag cgtggacttg ccggacccgg aggggccgat caccgcgacg
     2761 acctcgccgt tggcgacggt caggtcgatg ccggtgagca cctcgttctt gccgaaggac
     2821 ttgtgcaggt cgcgcacctc gatcgggttg cccgaagcgg tggtgacggg acgtgtgaac
     2881 gtctgctcgt tgttcgtcat ggtcatcccc tacttgttga acttccggtc gacccagttc
     2941 gagagaacgg tcagcagcca gatcacgatg aagtacatcg cgccgacgat gatccagatc
     3001 tcgaacgacc ggaacgtgga cgcgatcacg atgcggccct ggtacgtcag ctcggcgaag
     3061 cccagcaccg agagcagcga ggtgtccttc agcgtgatga tgaactggtt gatgaggttg
     3121 ggcgtcatga tcttgaacgc ctgcggcatc accacgcgac gcatcgacgc gccccagccg
     3181 agacccagcg agcgcgaggc ctccatctgg ccggggtcga ccgactgcac gccgcctcgg
     3241 acgatctcgg tgatgtacgc acccgcgttc agcgacagcg tgatcacgcc ggcggtcagg
     3301 gcgtccaggg gctggccggt cacggccggc acgccgaagt agaagaagaa cgcctgcacc
     3361 agcaccgggg tgccgcggaa gatgttgacg taggcgctgg cgaggaagcg caggaacggg
     3421 ttcgtcgaca gcttcaggat gccgaacagg atgcccagcg ccatggcgaa caggatcgac
     3481 agcgccgtgg ccagcagcgt gagcagcaga ccctgggaca gcgggccgat cgacgtcttc
     3541 agcagcgaca ggaaggagtt gtcggccgtc gcgctcgcgt cgtcgatcgc gaggtagcgg
     3601 tccacgatct gctggtaggc gccggtgtcc ttggcgttct gcaggccggc gttgaacatc
     3661 ttcagcagct cggcgttctg acccttgagc acggcgaagc cgtagtcgga gccgggctct
     3721 gcgtcggtga cgatcttcag cggcacgttg cccgaggcga tgccgaactg cagcacgggt
     3781 gcgtcctcga acgcggcgac cgagttgccg gtggccacgt cgttgtacat gtcggcggag
     3841 tcctggaacg cattgaccgt gaagccgatc tcggtgccga gcttcttggc gaagtcgtag
     3901 ccgaccgtgc ctgtcttgac ggcgaccttc ttgcccttga ggtccttgta cgaggtgatg
     3961 tcgctgtcct tcgcgacggc catctgcacg ccggactggt agtaggggtt cgagaagtcg
     4021 aacacctgct tgcgctcatc ggtgatgctc atgccggcga tcacgccgtc ggcctgaccg
     4081 gccttgaccg cctgaagcgc cgcgtcgaag cccagcgtct tcacgttcac ggtgaagccc
     4141 tggttcgcgg cgatcgcgcg catcaggtcc atgtcgatgc cgacgttctt gccgtccagc
     4201 tggtagatga acggggcgaa cgtggtgtcg gtggcgatgg tgaactcgcc atgggtctgc
     4261 gcggtgtcgg tcgcgaaggc cgggttcttc ggcagctcgc ccggcttgcc cgggtcgagc
     4321 ttgtcgttgc cgagtgcggc cttgccctcg ccgatcgctg ccgccttcgg ggcgttctcg
     4381 ccgaggtagt cgtcgaggat cttctggtag gtgccgtcgg cgatcaggtt cttcaggccc
     4441 gcgttgaacg ccttgcggag cgcctcgttg gcgcccttct tgacgcccat gccgtagctg
     4501 gacgccttct cctgcggcgt gaccgtcttc aggtcgacgt tgccggtggc gatgccgtag
     4561 gccaggatcg ggtagtcgtc gaacacggcg gcggagttgc cggtggtgac gtcgttgtag
     4621 gcgtcggcgg cgtcctggaa cccggtcacg gtgaagccgt actgcttgcc caggttctcg
     4681 gcgaaggtgt agccctcggt gccggtcttc gcggagacgg tcttgccctt gaggtcctcg
     4741 tagcccttga tgctcgcgtc gctcttggcc acagccatct ggatgccgga gtcgaagtac
     4801 gggttcgaga agtcgaaggt cttctggcgc tccttcgtga tggacatgcc cgccatcacg
     4861 gcatccgcct gcccggcggt cactgcggcg agtgcgccgt cgaagccgag cacgtcgacc
     4921 ttgacgtcga agccctggtc ctcggcgacg gccttgatca ggtcgatgtc gataccgcgc
     4981 agctcgccgt cccgctggaa ctcgaacggc gcccaggtgg tgtccgtcgc gatcgtgaag
     5041 gtcttgccct tgacgccgtc gtcggcggag gcgggcttgg tcgcggcgtt ggcgatgccg
     5101 accgcaccgc ccaccgtgaa cagcaggatg agcagcgtgg tgaggctcgc cagtagcggc
     5161 aggcgattct cacgccacca gttcgcaggg tgtctgagca gagtcatgga gtcctcttcg
     5221 tcgtgccgtc ttcggcatcg tgcgtgtttc cggcccgtgc caccttaccc gcccgcggaa
     5281 tcccggcctg gttctctgac gtgccgccgg gacggcggag gtgccggcac gtattcgccg
     5341 cacagcgaaa gatggcacga ttgaaccgcg tcgcatcact ccggggaggt ctgccgatga
     5401 gcgcctccgt cacggtccca gccgtcacag tcatcgtccc gacgtacaac gaacgcgaca
     5461 acgtcgccga gctcgtcgcc cgcaccgccg ccgcgctgga gggcttcgac gccgagatcc
     5521 tcttcgtcga cgacagctcc gacggcacca tggacgagat cgggcgggtc gcggcatccg
     5581 ctccgattcc ggtccgcggc atccaccgtg agcacaacac cgggggactc ggcggcgccg
     5641 tggtgcgggg gctttccgag gccgcgcacg acgtctgcat cgtgatggac ggcgatctgc
     5701 agcatccgcc cgagctcctg cccaccctgc tggcccggtt cggaaccggg gatgccgatg
     5761 tcgtcgccgc ctcgcgctac atcggcggag gcgacaccgc gggactcggc acgtcggtgc
     5821 gactgggggt ctcgaaggcc gcgaccgcgg tgaccaaggc gatgttcctg cgccggctgt
     5881 ggcgcagcac cgatccgatg acgggtttct tcctggtcga ccgctcgcgg ctcgacgtgg
     5941 gcacgctgcg ccccgagggc ttcaagatcc tgctggagat cctcgtgcgc gggggctcgg
     6001 ccgggccgct gcggatcgcc gaggtgccga tggagttcgg cgcgcgccgg cacggcacct
     6061 cgaaggcgag cctgcggcag ggggccatgt tcctggcgca cctggcccgg ctgcgcttcg
     6121 gcaagatggg cctgttcgcg atgatcggcg gcatcggcgc ggtcgccaac atcggaatca
     6181 tgtgggcgct cacgcacctc ggcatggact acgtgctcgc ggcggtgatc ggcgcggtcg
     6241 tgaccatcgc cggcaacttc gtgctgcagg agctgttcgt cttccgcgac gagcgcgcgg
     6301 acgcctcccg gctgtgggtg cgcttcgcgg catccgcgtc gttcaacggc atcgaggcgg
     6361 cgctgcgcat ccccgtgatg gcgctgatgg tggagtcctg gcacatctcg gcggtggtcg
     6421 ccaccgcgat ctcgctcgtc gtggcgttct tcgcgcggtt cctgttccat gcgctggtcg
     6481 tctacgcacc gcggaggcgc cgggcggata gtgcggagga gccgcccgtc gacaccgcgg
     6541 ccatccgtgt gctcaaggcc atcgacgcgg aggccatgcg cccgggcgag ctctgacccc
     6601 cggggtgctg cgtgcgcgcc cggtcgcatt atcctgggcc ggtgaccgac tacgcgactg
     6661 gggccagatt ccgactcggg cggatgctgc cgcgtgaccc gcacgagccg caccgcgtcg
     6721 cgagctcgct cgagctcttc ttcgacctgg tcttcgtgat ctcggtgagc accgcctcca
     6781 gccagctgca tcacgcgctg tccgaagggc acgcggcgac ggggatcacc tcgtatctga
     6841 tgatcttctt cgcgatctgg tgggcgtgga tgaacttcac ctggttcgcg acgtcgttcg
     6901 acgtcgacga ctggctgtat cgcgtcgtca cgatcgtgca gatggccggg gtgctcgtga
     6961 tggccgcagg catcgctccg gtcttcgacg cgcacgagcc cgatttcacg atcgtcgtgg
     7021 tggcgtacgt cgtgatgcgc gtcgccatga tcgtgcagtg gctgcgggcg tcccgcacca
     7081 accccgcgct gcgccgcacc gctctgggct acgccatcgg gatcggcgcc gtgcaggtgc
     7141 tgtgggtggg gttcctcttc atcccgaaca gcatccagct gccggtgttc atcgtgctga
     7201 tgctcgtcga gatctccgtg ccgatcatcg ccgagcggca ggggtcgacg ccctggcatc
     7261 cgcaccacat caccgagcgc tacagcctgt tcaccctcat cgtgctgggc gagagcctgc
     7321 tggcctcggc caccgcgatc atcggcgcgc gggaggaggt cgactccctc ggcccgctga
     7381 tctggatcgc ggcggtcacg ctgatctcgg ccgcggcgat gtggtggatc tacttctggc
     7441 ccccgcacca tcgagcgatc tcgaccctgc gcagctcgct cgtctacggc tacgcgcact
     7501 acttcgtctt cgccgcggcc gcggccttct ccgccggggt ggaggtgcag atcgacgagc
     7561 tcaccggcca cacggagctg ggtttcgtcc ccgcctcctt cacgaccagc gtgccgatcg
     7621 cggtgttcct gctcgggatc tggatcgtgg cgatccgcga caacgcggac cgcgtggtga
     7681 acacggtggt gccggtcggc gcggtgctgg tgctgctcga cgcggtgctt cccgtgccgg
     7741 tcaccctcac cgcggtgatc ctggtcgcca tcgtggtggt gctcgtgctg cgtccgccgg
     7801 tgcgcaagga gcagagcgcc cgctgacccg cgcgtgctgc cggggcgttc cgcggcgcct
     7861 cgcggcatcg cgggtgcgtt cggctgttga catgacgtcg ccggcccatt actgtcatct
     7921 gcagttattg atcgtctgat caatacgtcg aggaggacag catgggatgg cggatgccgc
     7981 acgagggcga cccgcacgag cgcacctgga tgaccttccc tcgcgccggc accacgctcg
     8041 gcatggggga tgagcaccgg gagagcggct accgggcgtg ggccgacacc gcgctcgccg
     8101 tggccgagtt cgaacccgtc accctgctcg tcgacccgac cgagaccgac cgcgctcgcc
     8161 gcatgctcgg gacgacggtc gacatcgtcg aggcgcccgt ggacgagttc tggctgcgcg
     8221 actccggccc caccttcgtc gtcgacgacg agcgacccgg cgtgctcggc gccgtggact
     8281 ggatcttcaa cggctggggg gccccggcct gggcggagtg gcagaaggcg gcccagcacg
     8341 cccggatcgt cgccggtcac gtcggcgccg agatcgtctc gagcctgctg gtgaacgagg
     8401 gcggcggcat ccacgtcgac ggcgagggca ccgtactgct caccgagacc gtgcagctcg
     8461 acccgaaccg caatccctac gccgacaggg cccgcgtcga ggccgagctc gcgcgcacga
     8521 tcggcgcatc caaggccatc tggctgcccc gaggcctcac gcgcgactac gacgagttcg
     8581 gaaccaacgg gcatgtcgac atcgtcgccg cgttcgccgg ccccggccgc gtgctgctgc
     8641 accgccagga cgacgccggt caccccgacc acgcggtcac gcgggagctg aaggccttcc
     8701 tgcaggacca gacggatgcc gcgggccgcc cgctcgagat catcgacgtg ccggcgccgg
     8761 cgaccctgcg cgacgacgag gggttcgtcg actggagcta catcaaccac ctcgtggtca
     8821 acgatgccgt gatcgcgtgc gggttcggcg aggacgccgc cgatgcccgc gcacgggaca
     8881 tcctcgaagc cgcctacccc ggccgacggg ccgtgacggt cgacgcgcgc gcgatcttcg
     8941 cgcgcggcgg cggcatc
//
LOCUS       DATMSX010000159         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_115974,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000159 DATMSX010000000
VERSION     DATMSX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115974"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VN041_04845"
     CDS             <1..345
                     /locus_tag="VN041_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWU28407.1"
                     /translation="RDRSLAEAALHAYTEVGADHGAARARASFRQAGVPVALPARSRP
                     ASGWESLTRTEERIAGHVATGETNLEIAQSLFVSRRTVETHVSNILAKLGLRSRTELA
                     IRFAHRLDQAGQ"
     gene            696..1406
                     /locus_tag="VN041_04850"
     CDS             696..1406
                     /locus_tag="VN041_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWU28408.1"
                     /translation="MTASAPPSPNPAARAGAVYFLGRAILRPLFWVMYRPRIVGRTNV
                     PDHGPVLLVSNHLSSLDTVIIPTSAIRPVQFLIKASYFAGSGVLGRFKRWFFGSIGGV
                     PVHRTTGRDARAALDAGSSILRAGSVFAVFPEGTRSRDGKLHEGHGGAAWMARDTGAA
                     VVPVGLIGTGSVRPLSHLLPGRPRLEVRFGEPLDLTDLAGMPDGRARREITERMMAAI
                     ARLSGQERSGSVNASSRD"
     gene            complement(1444..2025)
                     /locus_tag="VN041_04855"
     CDS             complement(1444..2025)
                     /locus_tag="VN041_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28409.1"
                     /translation="MRLAEVERGDTLRHRLLIRMISRFAGYRLPDAARVAFYDRDFAG
                     RALGEWTNRAMRGRSAWSVSERELMAATVATWNECPFCVGAHRAVAVLGMDDAVAAAV
                     LEDYRTAPISDRLRAALAFLDRMTNNPDDLGGDDVRVALDAGLSVDDLRDAAAVGAVF
                     NIITRNANALDFEIPSPGDFARAATMLLRRGYG"
     gene            complement(2012..2242)
                     /locus_tag="VN041_04860"
     CDS             complement(2012..2242)
                     /locus_tag="VN041_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28410.1"
                     /translation="MSAKSPASLTEPFRTLAEQVGRASFRVTDAQVDAVRDAAGSEKA
                     AFEIVMAASVGAGLRRWDAAMRAIEEAGDAAR"
BASE COUNT          345 a          817 c          775 g          349 t
ORIGIN      
        1 cgtgacaggt cgctcgcgga ggcggcgctg cacgcctaca ccgaggtcgg cgccgatcac
       61 ggggcggcac gcgcccgggc atccttccgg caggcggggg taccggtggc gctgccggcg
      121 cggtcgcgac ctgcatccgg ctgggagtcg ttgacccgaa ccgaggagcg gatcgccggt
      181 cacgtggcca ctggcgagac gaatctcgag atcgcgcaga gcctgttcgt ctcgcggcgc
      241 actgtcgaga cgcacgtgtc aaacatcctc gccaagctcg gcctgcgctc gcggaccgag
      301 ctggccatcc gcttcgcgca tcggctcgac caggcgggtc aatgacgcca tcagatcgat
      361 ccaaccgctg aagcgcagtt cgagcgcgga gtcgccgacc cgcccctcgg gagggtcggc
      421 gccatccagc cagatgtcga tgtgcacgct gccagggtcg tcccgcccgc gggcagggac
      481 catccgtgag atcacggaag ttccgcagcg gctctgttcg gaggatgcgc gcgggtgtag
      541 tttcagcgca tcgtgcatcc gctggggaga ggatgcacga cgcggatgcc gaatgctggg
      601 gacggcatcc gcctttcact tgtcgatcga cgatgcgcat ccgcacaccg ccccctccgc
      661 tgcattcagc atccacggac ggatagggtg gcgaggtgac agcatccgct ccgccgagcc
      721 cgaatcccgc cgcgcgcgcc ggtgccgtct acttcctcgg acgggcgatc ctcaggccgc
      781 tgttctgggt gatgtaccgt ccgcgcatcg tcgggcggac gaacgtgccc gatcacggcc
      841 ccgtgctgct ggtgagcaac catctgtcgt ccctcgacac cgtcatcatc ccgacctccg
      901 ccatccgccc cgtgcagttc ctgatcaagg cgtcgtactt cgcgggttcc ggggtgctcg
      961 gacgcttcaa gcgctggttc ttcggttcga tcgggggcgt cccggtgcat cgcacgaccg
     1021 gtcgggacgc gcgtgccgcg ctcgacgcgg ggagcagcat cctgcgggcc gggagcgtgt
     1081 tcgccgtgtt cccggagggg acgcggtcgc gcgacgggaa gcttcacgag ggtcacggcg
     1141 gggcggcgtg gatggcgcgc gacacgggag ccgcagtcgt gcccgtcggg ctgatcggga
     1201 ccggctccgt cagaccgctg agccatctgc tgcccggcag accgcgcctc gaagtgcgct
     1261 tcggcgaacc gctcgacctc accgatctcg cggggatgcc cgacggcagg gctcgtcgcg
     1321 agatcaccga gcgcatgatg gccgccatcg cgcgactgag cgggcaagag cgctccggca
     1381 gcgtgaacgc gagttcgcgc gactgagcgg cgtcctcgcg cgtcttgcgt cagcgactcg
     1441 cggtcagccg tacccgcggc ggaggagcat cgtcgccgca cgggcgaagt cacccggtga
     1501 cgggatctcg aagtccagtg cgttggcgtt gcgggtgatg atgttgaaga cagccccgac
     1561 cgcagccgca tcgcgcagat cgtccacgct cagccccgcg tccaacgcga cccgcacatc
     1621 gtcaccaccg agatcgtcgg ggttgttcgt catccggtcc agaaaggcca gcgcggcgcg
     1681 cagccggtcg ctgatcggcg ccgtccggta gtcctcgagc accgccgccg cgacggcgtc
     1741 gtccatgccg agcaccgcga cagcacggtg cgcgccgacg cagaacgggc actcgttcca
     1801 cgtcgcgacg gtcgccgcca tcagttcccg ttccgacacc gaccaggcgc tgcgtccgcg
     1861 catcgcgcgg ttggtccatt cccccagtgc ccgccccgcg aagtcgcggt cgtagaaggc
     1921 gacgcgggcg gcatccggaa gccgataacc cgcgaagcgc gagatcatcc ggatgagaag
     1981 ccgatgccgc agcgtgtcgc cgcgctcgac ctcagcgagc cgcatcgccc gcctcctcga
     2041 tcgcccgcat cgcggcatcc caccgtctca gccccgcacc gacgctcgcc gccatcacga
     2101 tctcgaaggc ggccttctcc gagcctgccg cgtcacgcac ggcgtccacc tgggcatccg
     2161 tgacgcggaa agacgcgcgc ccgacctgct cagcgagcgt ccggaagggt tcggtcagac
     2221 tggctggcga ctttgcgctc atctacaaag cacatggcgg cgtttatcta tccggcggta
     2281 ttccgc
//
LOCUS       DATMSX010000160        10234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_30584,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000160 DATMSX010000000
VERSION     DATMSX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30584"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VN041_04865"
     CDS             complement(<1..134)
                     /locus_tag="VN041_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28411.1"
                     /translation="MRASERAYAALLDEIQSGALPSGAVLAEVEQAARLGVSRTPLRE
                     A"
     gene            complement(220..819)
                     /locus_tag="VN041_04870"
     CDS             complement(220..819)
                     /locus_tag="VN041_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005507548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU28412.1"
                     /translation="MVAVTLVIAGAAGLIVGAVLYATRPGNLFANPVVFQTLNIVINL
                     VRPIPFIIFLTAVGPLTKAVVGTTLGTTAAIVPMVVMATVVIARVVEQNLVAVDPGIV
                     EAASAIGARRLSILFGVVIPEALAPLILGYTFMFIAVVDMSAMAGYIGGGGLGNFAII
                     YGYQQFDQGATWITVAIIVVIVQVGQFIGNRLAQRILRH"
     gene            complement(888..1937)
                     /locus_tag="VN041_04875"
     CDS             complement(888..1937)
                     /locus_tag="VN041_04875"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HWU28413.1"
                     /translation="MSSVISFEGVTKAFPGPKRAPELVAVDDVSLAVQAGSIVGVIGY
                     SGAGKSTLVRLINGLEKPSSGSVNVLGVDVGAASPSALRALRGRIGMIFQQFNLFNAR
                     TVRGNVGYPLKVAGWKRADIDRRVDELLDFVGIGDKASQHPRKLSGGQKQRVGIARAI
                     ATEPEILLADEATSALDPQTTAEVLALLRRINRQLGVTIVVITHQISIVHELCDQVIV
                     MDGGRVVDSGETYRVFAHPEAALTERFVAAVTQGLPEGETLTALLSGGGVLLSVDVNE
                     VTTEDIAAVLDAHGLRGTVVYGGVADVLGRQLGTLTYRIRAEQDAVRSAAAELSQRTK
                     ATIFTGADAHAAVGP"
     gene            complement(1934..2842)
                     /locus_tag="VN041_04880"
     CDS             complement(1934..2842)
                     /locus_tag="VN041_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU28414.1"
                     /translation="MTKNRAPRILTALAVLAAGMMAFAGCAAAASEPSSSSSGTQKIV
                     LGADDATEAHWGILKDKLAKEGIELEVRNFTDGVQLNNGVKDGDLDINLFQHLIFLAQ
                     YDVDNKADLVPIGATAVYPLALYSEKYSSVDDLPEGATVALPNNPTNLARALLNLQTA
                     GLLTLKDGGNALSTPADVVTSRIELKPVDSNQTVTALKDGSAQAAVVNNTQAQRGGLG
                     DDRIIFKEDLDDPKLAPYINAFVVKADRKDDKVWQKIIDAYHSTEVEASVTELNQGNL
                     KFKAEWTSAQLQDELTRLEDDIRAHK"
     gene            3013..3369
                     /locus_tag="VN041_04885"
     CDS             3013..3369
                     /locus_tag="VN041_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWU28415.1"
                     /translation="MGKQMTEMLKGTLEGIVLALLSGRPAYGYEITTQLRDRGFEEIA
                     EGTVYALLVRIEQKGLVDIEKVPSEKGPPRKVFTLNAQGEQELAEFWRTWSFLADRIE
                     QLHSPAASENPSEGEN"
     gene            3371..3739
                     /locus_tag="VN041_04890"
     CDS             3371..3739
                     /locus_tag="VN041_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HWU28416.1"
                     /translation="MAMKWIEALTGSLEQKKQYRQDKARIDGLAEPYGSAAKAMHRYL
                     LNTSGIVDGDIIIRMLTDLADLWETAAVDGTPVHDIVGDDPADFADTFARAYAAKEWQ
                     DKERARFVKALEDAKKEERS"
     gene            3736..4524
                     /locus_tag="VN041_04895"
     CDS             3736..4524
                     /locus_tag="VN041_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28417.1"
                     /translation="MNDARTDAGTAISVRGIEKSFKDLHVLRGVDFEVRKGSIFALLG
                     SNGAGKTTLVRILSTLSKADAGAATVHGFDVTSEPLDVRRSISLTGQFAAVDEILTGR
                     ENLVLVGKLRHLPDPGAVADALLARFRLTDAADRRAGTYSGGMRRRLDIAMSLVGEPE
                     VIYLDEPTTGLDPEARIEVWNVVKELADAGRTVLLTTQYLDEAEQLADRIAILHEGRI
                     IANGTLAELKQLLPAAKVEYVEKQPTLEEIFLALIGPESERSAA"
     gene            4521..5288
                     /locus_tag="VN041_04900"
     CDS             4521..5288
                     /locus_tag="VN041_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28418.1"
                     /translation="MSTHFVADTATLTGRSMRHILRSADTIITTAVTPIALMLLFVFV
                     FGGALKQSIGEGSYVNYLLPGILLIAIASGIAYTAFRLFTDLQGGIFERFHSMPISRS
                     SVLWAHVLTSLAANGITLVIIFAVGFLTGFRTGASVWAWLGVIGILALFTLALTWLAV
                     IAGLSAKTVDGASAFSYPLIFLPFISSAFVPTETMPGPVRWFAENQPVTSIVNTIQAL
                     FAGQPVGSSIWIALAWCVGILVIAYVFAMRAYRRRIG"
     gene            5452..6141
                     /locus_tag="VN041_04905"
     CDS             5452..6141
                     /locus_tag="VN041_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWU28419.1"
                     /translation="MQTIGVLGGMSWESSLDWYRIANEKVREELGSFHSAPILLDSLD
                     FAEIEAMQASGDWDAAGEVLAAHAKRLEAAGAGLVVLCTNTMHLVADRITDAIGVPFL
                     HIADTTAEAISAAGLQTVGLLGTAFTMEQTFYRDRLGSHGITALIPDAEDRATVHRII
                     YDELVQGIVTEESRRKYRGVIERLVDAGAQGVILGCTEIELLISQQDSAVPVFPTTAL
                     HVAAALKAAGL"
     gene            6228..7586
                     /locus_tag="VN041_04910"
     CDS             6228..7586
                     /locus_tag="VN041_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWU28420.1"
                     /translation="MEISSTRPTEHRDVLSGLVEMAQSIEASIDGLLAARDGVLALAA
                     RVALSQASDADDADLQVRSVAAELGAALRVSDRTIQRRMVAAEVKIDRFPAVWAAQGA
                     GRISAGHARVIVDAGERIEDADARAAYAQRMVAFAEGESPNRTRVVAERLAEQCQPRS
                     LQERHADAREQRGAWVKDRPDAMAEFGLYGPAVLVHAAFERLTGMGKEVKKRTAPEAG
                     GEGPDADPRTFGQVRADLALDLLLTGAPVGHDTPDGMLAAVTGHVAVTVPVLTLMGPG
                     DEAGSAPAAELTGRSPIDPATARMLVGAASGWDRVLTDPISGELLAVDRYRPSEHLKR
                     HLRARDERCRFPGCGTPARDADLDHTLDAAHGGPTEDGNLSALCRRHHVLTHHSPWKA
                     KQIGGGLLEWTSPAGRSYVDRPPPQNTVVFTPSGTDTGEPGGGHPPGLWAALPRDLHS
                     PF"
     gene            complement(7729..8508)
                     /locus_tag="VN041_04915"
     CDS             complement(7729..8508)
                     /locus_tag="VN041_04915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28421.1"
                     /translation="MHEELLQAYGDQLRGAAEVQGEAEVNRLGPLWLAQFRGDRGFIT
                     YRSLPADAREIAELVDAALEHFRERTSVTAVEWKTRGHDAAPGLEDTLRERGFVAEEP
                     ESIMIGRAEGLIGKVELPEGVEVRRASSDDDIRASYEMQGLVFDDPDWRARADDAVRR
                     REAGEQQELWIAVADGAVVSTGRLEPVDGTDFAGIWGGATLPEWRGRGIYRALTSRRA
                     EVALALGKTLINSDSTEFSRPILERSGLVKVSTTTPYVWTR"
     gene            8605..9282
                     /locus_tag="VN041_04920"
     CDS             8605..9282
                     /locus_tag="VN041_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28422.1"
                     /translation="MRILLVDDEVRLADGIRRGLEAEGMLVDVAHNGVDGLRMATDVD
                     YDAIVLDVMMPGMSGYRVCQALRADDIWTPVLFLTAKDGEWDEVEGLDTGGNDWLTKP
                     FSYPVLVARLRALVRRGGRERPTVLEAGDLRLDPAARRVFRGDAEVTLTARELSVLDF
                     LMRRRDEVVTKREVIANVWGDDFDGDANIVEVYIGHLRAKLDRPFGREAIETVRGAGY
                     RLSGTGG"
     gene            9284..>10234
                     /locus_tag="VN041_04925"
     CDS             9284..>10234
                     /locus_tag="VN041_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWU28423.1"
                     /translation="MPGVPFSRSIRARVTAGAFIVVAIVLGIAAVSTVQILAGALTDG
                     VRVSVEQDLETIGDQLEDGTSAARTVDGIGDDVLVRLSGSAPVVNDEDAAALPMVAED
                     STARVVIDGDAYLAASEDTDAGMLTVARPLEHVDDAVSTATVLLAVGVPLVLVLLAVV
                     VWVVAGRALAPVERLRRQVDGIGATELSRRVEAGDDELGALAETMNRMLARIEHAQNV
                     QRRFVSDASHELRSPLATIRQHAELADAHPDSTTLEELSRVVRDEGTRMQDLVEGLLL
                     LARLDERQQQPSGPVDLDDLALDEAARLRGMGVQVDTTGVG"
BASE COUNT         1615 a         3521 c         3500 g         1598 t
ORIGIN      
        1 gcttcgcgca gcggcgtgcg gctgacgccg aggcgcgcgg cctgctcgac ctcggcgagc
       61 acggcgccgg agggaagagc cccggactgg atctcgtcga ggagcgcagc atacgctcgt
      121 tcactggctc gcacgccatc agtgtataca taagcgcgtc agaacatcaa tcagacctgg
      181 ctatcatcct ctattcgtat acattcaacg cgacagcttc tagtgacgca ggatgcgctg
      241 ggcgaggcgg ttcccgatga actggcccac ctgcacgatc acgacgatga tcgccacggt
      301 gatccaggtc gccccctgat cgaactgctg gtagccgtag atgatcgcga agttgcccag
      361 cccaccgccc ccgatgtagc cggccatcgc cgacatgtcg accacggcga tgaacatgaa
      421 ggtgtagccg aggatgagcg gggcgagcgc ctcggggatg accacgccga acaggatgct
      481 cagccgcctg gcgccgatcg cactcgccgc ctccacgatc ccgggatcga ccgcgaccag
      541 gttctgctcg acgacgcgcg cgatgaccac ggtggccatc accaccatcg gcacgatcgc
      601 cgccgtggtg ccgagcgtgg tcccgaccac cgccttggtg agcggcccga ccgcggtgag
      661 gaagatgatg aacgggatcg gccggaccag gttgatgacg atgttgaggg tctggaacac
      721 gacggggttc gcgaacaggt tcccgggcct ggtggcgtac agcaccgcac cgacgatcag
      781 gccggccgcg ccggcgatca ccagggtgac ggcgaccatg aagaccgtct cctggatgga
      841 ctgcagcagg accggggtca gcgagtccca gctcgtggcg tcgcggatca cgggcccacc
      901 gccgcatgcg catccgcgcc cgtgaagatc gtcgccttcg tccgctgcga gagctcggcg
      961 gcggcgctcc gcacggcatc ctgctcggcc ctgatccggt aggtcagcgt gcccagctgg
     1021 cgcccgagga cgtcggcgac accgccgtag acgacggtgc cgcgcagacc gtgcgcgtcc
     1081 agcacggcgg cgatgtcctc ggtggtcacc tcgttcacgt ccacactcag cagcacgccg
     1141 cctcccgaga gaagcgcggt cagggtctcc ccctccggaa gcccttgcgt caccgccgcg
     1201 acgaacctct cggtgagcgc ggcctcgggg tgcgcgaaca cgcggtaggt ctcgccactg
     1261 tccaccactc tgccgccgtc catcacgatc acctggtcgc acagctcgtg gacgatcgag
     1321 atctggtggg tgatcaccac gatcgtcacc ccgagctgcc ggttgatgcg tctgagcagc
     1381 gcgagcacct cggcggtcgt ctgcgggtcc agtgcactgg tggcctcgtc ggccagcagg
     1441 atctcgggct cggtcgcgat cgccctggcg atgcccacgc gctgcttctg cccgccggag
     1501 agcttgcgcg gatgctgcga tgccttgtcg ccgatgccga cgaagtcgag cagctcgtcg
     1561 acccggcgat cgatgtcggc ccgcttccag cccgccacct tcagcgggta gccgacgttg
     1621 ccgcgcacgg tgcgcgcgtt gaacagattg aactgctgga agatcatgcc gatccggccg
     1681 cgcagcgcac ggagcgccga gggcgacgcc gccccgacgt cgacccccag cacgttcacg
     1741 ctgccactgc tgggcttctc cagcccgttg atcaggcgca ccagggtgga cttccccgcc
     1801 ccggagtatc cgatgacgcc gacgatggag cccgcctgga ctgcgaggct cacatcgtcg
     1861 accgccacca gttcgggggc gcgcttcggg ccggggaagg ccttggtcac gccctcgaag
     1921 ctgatgaccg agctcacttg tgcgcccgga tgtcgtcctc gagcctggtc agctcgtcct
     1981 gcagctgagc ggaggtccac tcggccttga acttcaggtt gccctgattg agctcggtca
     2041 cggacgcctc gacctcggtc gagtggtagg catcgatgat cttctgccag accttgtcgt
     2101 ccttgcgatc ggccttgacc acgaaggcat tgatgtacgg cgcgagcttc ggatcgtcga
     2161 ggtcctcctt gaagatgatc cggtcgtcgc cgagaccgcc gcgctgcgcc tgggtgttgt
     2221 tcaccacggc ggcctgtgcg ctgccgtcct tgagcgcggt gaccgtctga ttcgagtcga
     2281 cgggcttcag ctcgatccgg gaggtgacga catccgccgg ggtggacagc gcgttgccgc
     2341 cgtccttgag ggtgagcaga cccgccgtct gcaggttcag cagagcgcgc gccaggttgg
     2401 tgggattgtt gggcagcgcc acggtggcgc cctcagggag gtcgtcgacg ctggagtact
     2461 tctcggagta gagcgccagc gggtagacgg cggtcgctcc gatcggcacg aggtccgcct
     2521 tgttgtcgac gtcgtactgg gccaggaaga tcagatgctg gaacaggttg atgtcgaggt
     2581 cgccgtcctt cacgccgttg ttgagctgaa cgccgtcggt gaagttgcgg acctcgagct
     2641 cgatgccctc cttggcgagc ttgtccttga ggatccccca atgcgcctcg gtcgcatcat
     2701 cggcgccgag cacgatcttc tgggtgccgg acgacgagga cgacggctcg gatgctgcgg
     2761 ccgcgcagcc ggcgaacgcc atcatgccgg ccgccaggac ggcgagtgcg gtcaggatgc
     2821 gaggtgcgcg gttcttggtc atgtcgccat gcaaacagga agacggcgct gcgcgcactc
     2881 cggtcgtcat ggtgcgacac cccctgtcac ggaacgtcat gaaagtcgtc agtattcagt
     2941 cttgctaagt accactactc agtgttactt tgtaccggta gtcatcatca ctgagtagcg
     3001 gaaggagcgg ccatgggaaa gcagatgacc gagatgctca agggcaccct ggaaggcatc
     3061 gtgctcgcac tcctgtcggg cagaccggcg tacgggtacg agatcaccac ccagctgcgc
     3121 gatcgcggct tcgaggagat cgccgagggc accgtgtacg cgctgctcgt gcgcatcgag
     3181 cagaaggggc tcgtcgacat cgagaaggtg ccgagcgaga agggcccgcc gcgcaaggtg
     3241 ttcaccctca acgcccaggg cgagcaggag ctcgccgagt tctggaggac ctggagcttc
     3301 ctcgcagatc gcatcgaaca gctccactcc cccgcagcat ccgagaaccc ctccgaagga
     3361 gagaactgac atggccatga agtggatcga agcgctcacc ggatcgctcg agcagaagaa
     3421 gcagtaccgg caggacaagg cgcgcatcga cgggctcgcc gagccgtacg gcagcgcggc
     3481 caaggcgatg caccgctacc tgctgaacac cagcggcatc gtcgacggcg acatcatcat
     3541 ccgcatgctc accgacctcg ccgacctctg ggagaccgcc gccgtcgacg gcacccccgt
     3601 gcacgacatc gtcggcgatg acccggccga cttcgcggac acgttcgcga gggcgtacgc
     3661 ggcgaaggaa tggcaggaca aggagcgcgc acgcttcgtg aaggcgctcg aggacgcgaa
     3721 gaaggaggaa cgatcatgaa cgacgcacgc acagacgccg gcacggcgat ctcggtccgc
     3781 ggcatcgaga agtccttcaa ggacctgcac gtgctgcggg gcgtggactt cgaggtgcgc
     3841 aagggcagca tcttcgccct gctcggctcg aacggcgcag gcaagacgac gctcgtgcgc
     3901 atcctgtcga cgctgtcgaa ggcggatgcc ggcgcggcca ccgtgcacgg cttcgacgtg
     3961 acatccgagc cgctcgacgt gcgccgctcg atcagcctga ccgggcagtt cgccgcggtc
     4021 gacgagatcc tcaccggacg cgagaacctc gtgctggtcg gcaagctgcg gcatctgccc
     4081 gaccccggtg cggtggcgga tgcgctgctt gcccggttcc ggctcacgga tgccgccgac
     4141 aggcgcgccg gcacgtactc cggcggcatg cgccgccggc tcgacatcgc gatgagcctg
     4201 gtcggcgagc ccgaggtgat ctacctggac gagccgacca cggggctgga ccccgaggcg
     4261 cgtatcgagg tgtggaacgt cgtgaaggag ctggcggatg ccggtcgcac ggtgctgctg
     4321 accacgcagt acctcgacga ggcggagcag ctggccgacc gcatcgcgat cctgcacgag
     4381 ggtcgcatca tcgcgaacgg cacgctcgcc gagctgaagc agctgctgcc cgcggcgaag
     4441 gtcgagtacg tcgagaagca gcccacgctc gaggagatct tcctcgcgct gatcgggccc
     4501 gaatccgaga ggagcgcagc atgagcacgc acttcgtggc ggacaccgcc actctcaccg
     4561 gccggtcgat gcgccacatc ctgcgcagcg ccgacaccat catcacgacc gcggtcaccc
     4621 cgatcgcgct gatgctgctc ttcgtgttcg tcttcggcgg cgcgctgaag cagagcatcg
     4681 gggaggggag ctacgtgaac tacctgctcc cgggcatcct gctgatcgcc atcgcatccg
     4741 gcatcgccta cacggcgttc cggctgttca cggatctgca gggcggcatc ttcgagcggt
     4801 tccactccat gccgatctcc cgatcgagcg tgctgtgggc gcacgtgctg acctcgctgg
     4861 cggcgaacgg catcaccctg gtcatcatct tcgcggtcgg gttcctgacg ggtttccgga
     4921 cgggggcgag cgtgtgggca tggctgggcg tcatcggcat cctcgccctg ttcaccctgg
     4981 cgctgacctg gctggcggtg atcgcgggcc tgtccgcgaa gaccgtcgac ggcgcgagcg
     5041 cgttcagcta cccgctgatc ttcctgccgt tcatcagctc ggcgttcgtg ccgaccgaga
     5101 cgatgccggg tccggtgcgc tggttcgcgg agaaccagcc ggtcacctcg atcgtgaaca
     5161 ccatccaggc actgttcgcg ggacagccgg tcggctcgtc gatctggatc gcgctggcct
     5221 ggtgcgtcgg catcctcgtc atcgcgtacg tcttcgcgat gcgcgcctac cggaggcgga
     5281 tcggctgata tccggatgct gatcgccgac accccgtgtc gccgccgaga ccccgggttg
     5341 cggacctccg cagcccgggg tctgggcgat tctccggggt ctcggcgtcg tcctctgcgc
     5401 gatgccggcg cattctccct cactccatcc gccgaccgat agcgtgaggg catgcagacg
     5461 atcggcgtgc tcggcgggat gagctgggag tcctccctgg actggtatcg gatcgccaac
     5521 gagaaggtac gggaggaact cggcagcttc cactcggccc cgatcctgct ggactcgctc
     5581 gacttcgcgg agatcgaggc gatgcaggca tccggcgact gggatgcggc cggcgaggtc
     5641 ctcgccgccc acgcgaagag gctggaagcc gctggggccg ggctcgtcgt gctgtgcacg
     5701 aacaccatgc acctggtcgc cgaccggatc accgacgcga tcggcgtgcc gttcctgcac
     5761 atcgccgaca ccacggcaga agcgatcagc gcggcaggcc tgcagacggt cggcctgctc
     5821 gggaccgcct tcacgatgga gcagaccttc taccgcgacc gcctcggctc gcacggcatc
     5881 accgccctga tcccggatgc cgaggatcgt gcgaccgtgc accgcatcat ctacgacgaa
     5941 ctggtgcagg gcatcgtcac cgaggagtcg cggcggaagt accgcggtgt catcgagcgg
     6001 ctcgtcgacg cgggggccca gggcgtcatc ctcggatgca cggagatcga gctcctcatc
     6061 agccagcagg actccgcggt gccggtgttc ccgacgacgg cgttgcacgt ggcagccgcg
     6121 ctgaaggcgg cggggctctg aggggcgcct cgtgaggagc cgcgttccgc ggcattctcc
     6181 gttcgttcag atgtctgaat gtcggtggct cttctcatac ttgagtcatg gagatttcat
     6241 cgacccggcc gacggagcac agggacgtgc tgtcgggtct ggtcgagatg gcgcagagca
     6301 tcgaggcgtc gatcgacggg ctgctggcgg cgcgcgacgg ggtgctcgca ttggcggcac
     6361 gggtggcgtt gtctcaggcc tcggatgcgg atgatgcgga cctgcaggta cggtccgtgg
     6421 ccgctgaact cggggcggca ctgcgggtca gcgatcgcac gatccagcgg cggatggtgg
     6481 ccgctgaggt gaagatcgac cgcttcccgg cggtgtgggc ggcacagggt gcagggcgga
     6541 tcagtgccgg gcatgcgcgg gtgatcgtgg acgccggcga gcggatcgag gacgcggatg
     6601 cccgcgccgc gtatgcgcaa cggatggtcg cgttcgcgga gggcgagtcg ccgaaccgca
     6661 cccgggtggt cgccgagcgt ctggcggagc agtgccagcc gcggtctctg caggagcggc
     6721 atgcggatgc ccgtgagcag cgaggcgcgt gggtgaagga ccggccggac gcgatggccg
     6781 agttcgggct gtacggtccg gcggtgctgg tgcatgccgc gttcgaacgg ctgaccggca
     6841 tgggcaaaga ggtcaagaag cgcaccgccc cggaagcggg tggggagggc cccgatgcgg
     6901 atcctcgcac gttcgggcag gtgcgtgcgg acctcgcgct ggacctgctg ctcaccggtg
     6961 cacccgtcgg gcacgacacc cccgacggaa tgctcgccgc ggtcaccgga cacgtcgcgg
     7021 tcaccgtccc cgtgctgacg ctgatgggcc cgggcgatga ggccggctct gcgcccgccg
     7081 cagaactgac aggtcgttcc ccgatcgatc ccgccaccgc acggatgctg gtgggggccg
     7141 catccggatg ggatcgggtc ctgaccgacc cgatcagcgg ggaactgctc gccgtggacc
     7201 gctaccggcc ctcggaacat ctgaaacgcc accttcgcgc ccgagacgaa cgctgccgat
     7261 tccccggctg cggcacaccc gcacgagacg ccgatctcga tcacacgctc gacgcggcgc
     7321 acggcggccc cactgaggat ggcaacctgt ccgccctgtg ccgcaggcac cacgtcctca
     7381 cgcatcactc accgtggaag gcgaaacaga tcggcggcgg actgctcgaa tggaccagcc
     7441 cggcgggtcg cagctatgtc gaccggccgc cgccccagaa caccgtggtg ttcacaccgt
     7501 ccgggacgga cacaggtgag cccggcggcg ggcatccacc gggcctctgg gcagcgcttc
     7561 cgcgggacct ccactcaccc ttctgatgtg ggcgaccgtc ggcggtccgc ggcactcacg
     7621 cctggcggtt ggcatcctca cgcgaggccc gaccgatcac actcacttcc ggggttccgc
     7681 gagtccagcg cggagcctcg gatgcgtcga acgcaggcgc aaccggactc agcgggtcca
     7741 gacgtacggc gtggtcgtcg acaccttcac caggccggag cgctccagga tcgggcggga
     7801 gaactccgtc gagtcggagt tgatcagcgt cttgcccagc gcgagcgcca cctccgcgcg
     7861 ccgcgaggtc agcgcgcggt agatgccgcg accccgccac tccggcagcg tcgctccgcc
     7921 ccagatgccc gcgaagtccg tgccgtccac cggctccagg cgtcccgtgc tgacgacggc
     7981 cccatcggcc accgcgatcc acagctcctg ctgctcgccg gcctcccggc ggcgcacggc
     8041 gtcgtcagcg cgagcccgcc aatccgggtc gtcgaagacg aggccctgca tctcgtacga
     8101 cgcccggatg tcgtcatccg acgacgcccg ccgcacctcg acgccctccg gcagctcgac
     8161 cttcccgatg agcccctcgg cgcggccgat catgatggac tccggctcct ccgcgacgaa
     8221 gccccgctcg cgcagcgtgt cctcgaggcc gggcgcggca tcgtgtccac gggtcttcca
     8281 ctccaccgcg gtcaccgagg tgcgctcgcg gaagtgctcg agcgcggcat ccaccagctc
     8341 cgcgatctcc cgcgcatcgg ccggcagaga ccggtaggtg atgaagccgc gatcaccgcg
     8401 gaactgcgcg agccagaggg gaccgagccg attcacctcc gcctcgccct gcacctccgc
     8461 agcgccccgg agctggtcgc cgtacgcctg cagcagttcc tcatgcatcc gtcgatcctc
     8521 gcagatgacg acgtcggcgc ccacgaacga ggaccgtcat cattcttcag ccccgcttca
     8581 gcagcatccg gccagactgg gatcatgcgg atcctgctcg ttgacgatga ggtgcgcctc
     8641 gccgacggca tccgccgcgg tctcgaggcc gagggcatgc tcgtcgacgt cgcccacaac
     8701 ggcgtcgacg gcctgcggat ggccaccgac gtcgactacg acgcgatcgt gctcgacgtc
     8761 atgatgccgg gcatgagcgg ctaccgcgtc tgccaggcgc tgcgcgccga cgacatctgg
     8821 acgcccgtgc tgttcctcac cgccaaggac ggcgagtggg acgaggtcga gggtctggac
     8881 accggcggaa acgactggct caccaagccc ttctcctacc ccgtgctcgt cgcccggctg
     8941 cgggctctcg tccggcgcgg cggacgagag cgcccgacgg tgctcgaagc cggcgacctt
     9001 cgcctggatc ccgctgcccg gcgcgtcttc cgcggcgacg cggaggtgac gctgacggca
     9061 cgcgagctgt cggtgctcga cttcctgatg cgccgccgcg acgaggtcgt caccaagcgc
     9121 gaggtcatcg ccaacgtctg gggcgacgac ttcgacgggg atgccaacat cgtcgaggtg
     9181 tacatcggac atctgcgcgc caagctcgac cgtccgttcg gccgcgaggc catcgagacc
     9241 gtgcgcggcg ccggctaccg cctgtccggg accggcggct gagatgcccg gcgtgccctt
     9301 ctcgcggtcc atccgcgcac gcgtcaccgc cggcgccttc atcgtcgtcg cgatcgtgct
     9361 cgggatcgcg gcggtgtcga ccgtgcagat cctggccggt gcgctcaccg acggcgtccg
     9421 ggtctcggtg gagcaggatc tcgagacgat cggcgatcag ctcgaagatg gcacctcggc
     9481 cgcccgaacc gtcgacggca tcggcgatga cgtgctggtg cgcctgagcg ggtccgcgcc
     9541 tgtggtcaat gacgaggatg ccgctgccct ccccatggtc gcggaggaca gcaccgctcg
     9601 cgtggtgatc gacggtgacg cgtacctcgc cgcctccgag gacaccgatg ccggaatgct
     9661 cacggtcgcc cgcccgctcg agcacgtcga cgacgcggtg tccaccgcga ccgtgctgct
     9721 cgcggtgggc gtgcccctgg tgctcgtgct gctggcggtg gtcgtgtggg tggtcgccgg
     9781 ccgcgccctc gccccggtgg agcggctgcg caggcaggtc gacggtatcg gcgcgaccga
     9841 gctctcgcgc cgcgtcgaag ccggcgacga cgagctcggc gcgctggccg agacgatgaa
     9901 ccggatgctg gcgcgcatcg agcacgcgca gaacgtgcag cgccggttcg tcagcgacgc
     9961 ctcacacgag ctgcgctccc ccctcgccac gatccggcag cacgccgaac tcgccgacgc
    10021 gcatccggac tcgaccacac tggaggagct cagccgagtg gtgcgggacg agggcacccg
    10081 gatgcaggac ctggtggagg gtctgctgct gctcgcccgg ctggacgagc gccagcagca
    10141 gccctccggc cccgtcgacc tcgacgatct cgcgctcgat gaggccgccc ggctgcgagg
    10201 catgggcgtg caggtcgaca ccaccggtgt cggc
//
LOCUS       DATMSX010000161         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_59192,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000161 DATMSX010000000
VERSION     DATMSX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59192"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VN041_04930"
     CDS             complement(<1..403)
                     /locus_tag="VN041_04930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWU28424.1"
                     /translation="MGNRLSQIATRTGDDGTTGLGDNTRVPKDHLRVHAMGDVDELNS
                     HIGVLLCEPLPEPVRELLVDVQHQLFNLGGELSMPGFTLLKPEALLQIDNALADHNAV
                     LPRLAEFILPAGTRAASLAHVCRTVARRAERA"
     gene            complement(420..920)
                     /locus_tag="VN041_04935"
     CDS             complement(420..920)
                     /locus_tag="VN041_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWU28425.1"
                     /translation="MNPTTLAALAAFPSQLEAHYAAIPQAFRHWAPSSWEGVPSEAFT
                     AIEQLCHVRDIETEGYHVRFRRTLAETHPTLTSIDSEPLAIERNYGAADAATVLAEFR
                     LARVQTMEIVSQLSETQLARTAVFEGYGPLTMRSLIHYLCSHDQQHLAGLQWLAGKID
                     AAGLTT"
     gene            997..2421
                     /locus_tag="VN041_04940"
     CDS             997..2421
                     /locus_tag="VN041_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWU28426.1"
                     /translation="MNAPTSTSHLMPALHLRDVPAALIDGLKARFGDNCSTAMAVRTQ
                     HGRDESSFDAPPPSAVVFAESTQDVADAVKLASKHSVPVIPFGVGSSLEGHLLAVQGG
                     ISIDVSRMNKVLSINADDLTVTVQPGVTRKQLNEEIKSTGLFFPIDPGADASIGGMTA
                     TRASGTNAVRYGTMRENVLALEVVTAAGDVIRTGTRAKKSSAGYDLTRLMVGSEGTLG
                     VVTEVTLRIYPLPEAVSAAICSFPSIEAAVRTTIETIQLGVPIARVELIDVNTVRMVN
                     AYAKLGLREEPMLLMEFHGSPAGVKEQAETVQELASGHGGNAFEWASTPEERTRLWTA
                     RHNSYFAAVQSRPGCRVISTDTCVPISRLADCLLDSVAEADASGIPYFLVGHVGDGNF
                     HFGYLLDPNIPEERVKAEALNHALVSRALALEGTCTGEHGVGLHKMDFLVTEAGVGAI
                     DMMRTIKRALDPKNIMNPGKIFSL"
BASE COUNT          428 a          846 c          890 g          411 t
ORIGIN      
        1 ccgcgcgctc ggcacgccgc gccacggtgc ggcacacatg cgcgagcgat gcggcgcgcg
       61 tgccggcggg caggatgaac tcggccaggc gcggcaagac ggcgttgtga tcggccagtg
      121 cgttgtcgat ctgcagcagc gcctcgggct tgagcagcgt gaaacccggc atcgagagtt
      181 cgccgccgag attgaacagc tggtgctgta catccaccaa aagctcgcgc acgggctctg
      241 gcagcggctc gcacagcagc acgccgatgt gcgagttgag ttcgtccacg tcgcccatcg
      301 cgtgtacgcg caggtgatcc ttgggcacgc gggtgttgtc gcccaggccg gtggtgccgt
      361 cgtcgccggt gcgcgtggcg atctgggaaa gtcggttgcc catgggcgct cctctcggtt
      421 caggtcgtga ggccggcggc gtcgatcttg ccggccagcc attgcagtcc tgccagatgc
      481 tgctggtcgt gactgcacag gtagtggatg aggctgcgca tcgtgagcgg gccgtagcct
      541 tcgaacacgg ccgtgcgcgc aagctgcgtc tcgctcagct gcgagacgat ttccatcgtc
      601 tgcacgcgcg ccaggcggaa ctcggcgagc acggtggccg cgtcggctgc gccgtaattg
      661 cgttcgatgg ccagcggctc gctgtcgatc gacgtgagcg tcggatgggt ctcggcgagc
      721 gtgcggcgaa agcgcacgtg gtagccctcg gtctcgatgt cgcgcacatg gcacagctgc
      781 tcgatggcgg tgaaggcttc gctgggcacg ccctcccatg acgacggtgc ccagtgccgg
      841 aaggcctggg gaatggccgc gtagtgtgct tcaagctgcg agggaaaggc cgcaagggct
      901 gcaagggtcg tggggttcat gtcttctacc gaagcgcatt atggtgcgcg ctccgtaaaa
      961 tgaccgatcc ccctcagcac ctccaggaga cgcccgatga acgccccgac ttccacctcg
     1021 cacctgatgc cggcgctcca tctgcgcgat gtgcctgcgg ccctgatcga cgggctgaag
     1081 gcgcgcttcg gcgacaactg ttccacggcg atggcggtgc gcacgcagca cgggcgcgac
     1141 gagtcctcgt tcgacgcccc cccgccctcg gccgtggtgt tcgccgaaag cacgcaggac
     1201 gtggccgatg ccgtgaagct cgccagcaaa cacagcgtgc cggtcattcc tttcggcgtg
     1261 ggctcctcgc tcgaaggcca cctgctcgcg gtgcaaggcg gcatcagcat cgacgtgtcg
     1321 cgcatgaaca aggtgctgtc gatcaacgcc gacgacctca cggtgacagt gcagccgggc
     1381 gtcacgcgca agcagctcaa cgaagagatc aagagcacgg gcctgttctt ccccatcgat
     1441 ccaggcgcgg atgcctccat cggcggcatg acggccacgc gcgccagcgg cacgaacgcg
     1501 gtgcgctacg gcacgatgcg cgagaacgtg ctggcgctcg aagtggtcac ggccgcgggt
     1561 gacgtcattc gtaccggcac gcgcgccaag aagtcgtcgg cgggctacga cctcacgcgc
     1621 ctcatggtgg gcagcgaagg cacgctgggc gtggtcaccg aagtcacgct gcgcatctac
     1681 ccgctgcccg aagcggtgtc ggcggccatc tgctcgttcc cgagcatcga ggccgcggtg
     1741 cgcaccacca tcgagaccat ccagctcggc gtgccgatcg cgcgcgtgga actcatcgac
     1801 gtgaacacgg tgcgcatggt caacgcctac gccaagctgg gcctgcgcga agagccgatg
     1861 ctgctgatgg aattccacgg ctcgccggcc ggcgtgaagg agcaggccga gacggtgcag
     1921 gagctcgcga gcgggcacgg cggcaacgcc ttcgaatggg ccagcacgcc ggaagaacgc
     1981 acgcgcctgt ggaccgcgcg gcacaacagc tacttcgcgg ccgtgcagtc gcgccccggt
     2041 tgccgcgtga tctcgaccga cacctgcgtg ccgatctcgc gactggccga ttgcctgctc
     2101 gactcggtgg ccgaggccga tgcgagcggc atcccctact tcctcgtcgg ccacgtgggc
     2161 gacggcaact tccacttcgg ctacctgctc gacccgaaca tccctgaaga gcgcgtgaag
     2221 gccgaggcac tgaaccatgc gctggtgagc cgcgcgctgg cgctcgaggg cacctgcacg
     2281 ggcgagcatg gcgtaggcct gcacaagatg gacttcctgg tgaccgaggc gggcgtgggc
     2341 gcgatcgaca tgatgcgcac gatcaagcgc gcgctcgatc cgaagaacat catgaatccg
     2401 ggcaagatct tcagcctctg atcgatctca gtcccgcagg aaaaagcccg gcggctcgca
     2461 ggagcgccgg gctttttcgt ggcgggctga aatgaagacc gacggattct tgcttaccgg
     2521 cggcgacgcg ccggtgccgg ctccggcggc acgtaggtct gcgacggtgc cgcgc
//
LOCUS       DATMSX010000162        11818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_163141,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000162 DATMSX010000000
VERSION     DATMSX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163141"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VN041_04945"
     CDS             <1..623
                     /locus_tag="VN041_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWU28427.1"
                     /translation="GDAGYADGARLGRMLAQNHTVASGGGPGAMEAVNLGGYLAGHGP
                     EAIDEALEILARAPGFVPSIDAWVKAAFAVRELFPDGGDSLGIPTWHYGHEPPNAFAS
                     AIAKYFRNAQREAILLEICQDGIVFLPGAGGTVQEIFQDACENYYADESSTAPMVLIG
                     RDYWTRRYPAWPLLKALAKGRAMEPHVHLVDTVEDAVELMQPGVPR"
     gene            634..1473
                     /locus_tag="VN041_04950"
     CDS             634..1473
                     /locus_tag="VN041_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWU28428.1"
                     /translation="MAETLESVAADLCTTPLKSFVNERTAHAEAVGDPDLSRRIRALR
                     KPSVAAWVVNVFARERADRLGQALHLAEELRDAQADLDAGTLAELGRQRRALTNQLTR
                     EAAGLAENRGERVTATTLEAVRQTLSAAFYDPDAAAAVASGRLVRDLEPSEPVDLAAV
                     VAGGPPEQVAVAPRATDEVKARRERRDAERVVRDAEQELARARSEHGKADRNLHDATA
                     KADRLRARVVELESELTRLRHEAEIATETVDGAADRAARAAERMAEAERALADARAAL
                     DRL"
     gene            complement(1495..1791)
                     /locus_tag="VN041_04955"
     CDS             complement(1495..1791)
                     /locus_tag="VN041_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28429.1"
                     /translation="MGNLEYNSSRPPIQVDDITLAHLKVVIGTKLRRQESFMMTWLPD
                     QSVQSGRLTTWMHPAIPLIFSFDSTAMPVIDPKRIEHMMELLNARGDLVLDQMS"
     gene            1932..2207
                     /locus_tag="VN041_04960"
     CDS             1932..2207
                     /locus_tag="VN041_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28430.1"
                     /translation="MTQQPRALISIDGNDGIPIAPGEDIGDLKRRIEVAAVETRFVDF
                     STADGTQLSVLVSPYRRVSIAVIAPREPPDPTLLVDLSHLPPDLMDF"
     gene            complement(2513..2992)
                     /locus_tag="VN041_04965"
     CDS             complement(2513..2992)
                     /locus_tag="VN041_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28431.1"
                     /translation="MSDAVLLHVDEDGFFCLVAPDAYQGFVDEDWELEQLMTHFVGQM
                     REGSLFVAYPGPDDADEAVTVADDPPGAPALREAVSAVNVGAGGLWLTDYTQLTMAAQ
                     FDDEAPTAAGAIRLPVQEGSQLVTLSQVAGSPRYVLTIRPVADDNREPLSAVPWFSL"
     gene            complement(3438..3773)
                     /locus_tag="VN041_04970"
     CDS             complement(3438..3773)
                     /locus_tag="VN041_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28432.1"
                     /translation="MTDTPACAFKGCSEKPTNPIFVTRSPEQAGPWQALVCDFHRLAV
                     DSGEDFDFESETKELVSPLRLVNFAVASKGRGKTVTLMFGRDGVEDERTPEVRMDKEM
                     LQRIIDMLP"
     gene            complement(3999..4071)
                     /locus_tag="VN041_04975"
     tRNA            complement(3999..4071)
                     /locus_tag="VN041_04975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4036..4038),aa:Lys,seq:ctt)
     gene            4258..5067
                     /locus_tag="VN041_04980"
     CDS             4258..5067
                     /locus_tag="VN041_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWU28433.1"
                     /translation="MTVPLAATDFHADLDLALRLADAADEQSLPRFDAADLEVSLKAD
                     RSHVTDADLATERAIRDILHAERPDDGILGEEFGAEGVTSRQWIIDPIDGTANFLRGV
                     PLWGTMIALAVDGVPQVGVVSMPALGRRWWASAGDGSWTATSDGARRLSVSTVDSLDE
                     ASVSFQSISQWAQAGKTPELLALAERVWRDRAYGDIFSYMLLAEGRIDMVAEFDVKEY
                     DIAAAVPIVREAGGRMTAFDGTETLSALSALATNGILHDSFLSLFRDLPRA"
     gene            5099..5683
                     /locus_tag="VN041_04985"
     CDS             5099..5683
                     /locus_tag="VN041_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28434.1"
                     /translation="MKTRLPLAAGALLAALSLAACSGPSTPVDKTPTSTASKPPATPK
                     AEVETAPADKPATTTPADRPTCETMITKGTVEALKSQGWTFEEREFRIGDVLVDGGLE
                     CLWADYSTASDHGQMYAWGPLSAENSRAAQASLISDGWLRSTSNGKTYITEDPAYAIA
                     KDDDGFGMTYEFGDGWVRFADTKQGLLLVDWGEK"
     gene            complement(5771..5971)
                     /locus_tag="VN041_04990"
     CDS             complement(5771..5971)
                     /locus_tag="VN041_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28435.1"
                     /translation="MVDFIVFMVLFLGGFYLFGLAWELPTAQGVVFSAGILLVSLALA
                     YVMRQRGSATRRVDNWNQRGDK"
     gene            6089..6691
                     /locus_tag="VN041_04995"
     CDS             6089..6691
                     /locus_tag="VN041_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28436.1"
                     /translation="MTTSTRTARRGRPGHDRADVIRAGVELFNEQGYDATSVSDLTGR
                     LGLTKSALYHHVESKEQILQIALEDALGGLEKALEDALQQPTAGSRLTAIIEGAVRVL
                     TERQPQVTLLLRLRGNSEIETAALARRRRFDHTVTGLVREAQSEGIVRTDLDAGVATR
                     LIFGMINSVVEWYRPDGPVDPELLAGEILAVALGGLQPRA"
     gene            complement(6692..7312)
                     /locus_tag="VN041_05000"
     CDS             complement(6692..7312)
                     /locus_tag="VN041_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28437.1"
                     /translation="MSFFTPLEPEPPQERMQAPPTPIWLRPPTDEIPVAVPVARRLAR
                     VPGAALHLKRIDAHRDGIVVVLRLDIRLEDDLSPERRAEIEALLDPRRHRAGAERGFR
                     LGVTTAQGSAEQNGMDGRGIVRRMSGEPPQSPQLTMVDGGGSGTSDHWTSKVGAWLWP
                     LPAEGDVTLHYVSDAVGILEGSTTIDGELLREAARGVVRVWGDSSP"
     gene            7510..9369
                     /locus_tag="VN041_05005"
     CDS             7510..9369
                     /locus_tag="VN041_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWU28438.1"
                     /translation="MPSPNRAHRVASTILLTTAIAAVSGLTAATAAASTPAESNRDIK
                     TLVVGIDGASFDFLEPAGMPNLAALRADGLTATSNLYALPMSGTVSGPGWSTIATGVW
                     PDKHKVVDNSFSTPNYAEYPDYLTRIEQAAPERSTLVVGTWSPIPQTVFGPAVDERIA
                     GGDDAGTTARTVAALSTSDPDDVFVHLDEVDGAGHSVGTNGPAYGAALRRADSQLGEM
                     LAAIESRPTRASEDWLVIVTADHGHTPTGGHGGNTPAERKTFVIASGPGIVAGSVRND
                     VKLVDIAPTVLAADGIATDPAWNIDGSALGDLKADDFDSVRPALQPQLDETRPGTGVL
                     GWTHQTPEGWTIDNSAMPAGGVREWAGWSFATDDFYSNVERGQGRETNVRSRDVFAVA
                     DSDEWDDKAHDAGAFDSTLVSPTYPLTGASDAAVSFATTYVIDGPQSAEVLVSFDGAE
                     PVSLKNYAKNTNANERLEFAVPQGAQQAQFRFRYTGTNSAFWTVDRVAFQQAQPTAPA
                     APRALQVTSGDATAAASWQAPASDGHSPITAYTVTATPIVDKGKSKQATPLSVRTTAL
                     TATLNGLRNGVSYTVTVTATNAYGTSTASDVAVATPTVSRATPATPPSKPNKG"
     gene            complement(9450..9819)
                     /gene="ssrA"
                     /locus_tag="VN041_05010"
     tmRNA           complement(9450..9819)
                     /gene="ssrA"
                     /locus_tag="VN041_05010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9966..10631
                     /locus_tag="VN041_05015"
     CDS             9966..10631
                     /locus_tag="VN041_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWU28439.1"
                     /translation="MSDVIITVRGEHELRIAPERATVHLNIRAEGPQRVAAVDEVTRL
                     AEPVRQSIVDRKDAETVVEWSSTRMTVHAERPWNADGRQLAPVYHATIAFAATFPDPS
                     ELSLWVSDVSLRDGVEVGTVDWQLTPETRTRLERDVAAGAVSVAVARAEAYAAALGLD
                     SVVPLEVADLGLISRQESAPQPKMLMRSAAFAMDSAGASGMVYEAEDIVVSATVEARF
                     QAA"
     gene            complement(10657..>11818)
                     /gene="hflX"
                     /locus_tag="VN041_05020"
     CDS             complement(10657..>11818)
                     /gene="hflX"
                     /locus_tag="VN041_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWU28440.1"
                     /translation="AVLDGLLQRRPHPDPATYLGRGKAQELKDIVAAVGADTVIADTE
                     LAASQRRALEDVVKVKVIDRTTVILDIFSQHAKSREGKAQVELAQLEYLLPRLRGWGE
                     SMSRQAGGQVGAGGAGMGSRGPGETKIELDRRRIRTRMAQLRKQIREFAPARDAKRAE
                     RKRNTIPSVAIAGYTNAGKSSLLNALTSAGVLVENALFATLDATVRRAETHDGRIYTL
                     TDTVGFVRNLPHQLVEAFRSTLEEVGQADLIVHVVDGSHPDPSAQLQTVRDVMGDVGA
                     RSIPEIVVFNKADLVDEDERLVLRGLAPGAHFVSSRTGEGIDELREAIERMLPMPAVE
                     VRALVPYDRGDLVSAVHETGVLISAEHEEAGTMVHVHVSERLAAELAPYAVG"
BASE COUNT         1991 a         4131 c         3885 g         1811 t
ORIGIN      
        1 gcggcgacgc cggctacgcc gacggcgcgc gcctcgggcg gatgctggcg cagaaccaca
       61 ccgtggcctc gggcggtggt ccgggagcga tggaggcggt caacctcggc ggttatctcg
      121 ccggtcacgg cccggaagcc atcgacgagg cgctggagat tctcgccaga gcaccaggct
      181 tcgttccgag tatcgacgca tgggtgaagg cggcgttcgc ggtgcgtgag ctgttccccg
      241 atgggggcga ttccctcggc atccccacat ggcactacgg ccacgagccg cccaacgcgt
      301 tcgcgagcgc gatcgccaag tacttccgca acgcgcagcg cgaggcgatc ctgctggaga
      361 tctgccagga cgggatcgta ttcctgccgg gggccggcgg caccgtgcag gagatcttcc
      421 aggacgcctg cgagaactac tacgccgacg agtcgtcgac cgccccgatg gtgctgatcg
      481 gccgagacta ctggacccgt cgctatcccg cctggccact tctgaaggcg ctggcgaagg
      541 ggcgcgccat ggagccgcac gtgcatctgg tcgacacggt ggaggacgcc gtggagctca
      601 tgcagcctgg cgttccgcga taggagtcac gtgatggccg agaccttgga gagcgtcgcc
      661 gcagacctct gcactacgcc gctgaagagc ttcgtgaacg agcgcaccgc ccacgccgaa
      721 gcggtaggcg acccggacct gtccaggcgg atccgcgccc tgcggaagcc gtccgtcgca
      781 gcgtgggtgg tgaacgtgtt cgcccgcgag cgtgctgacc gactgggcca ggcgctgcac
      841 ctcgcagaag agctccgtga tgcccaagcc gatctcgacg ccggcacgct cgccgaactc
      901 gggcggcaac gacgcgcgct gacgaaccag ctcacaagag aggccgcggg gctcgcggag
      961 aaccgtggcg agcgggtgac ggcaacgacg ctggaggcgg tgcggcagac tctgtccgcc
     1021 gcgttctacg acccggatgc cgcggccgcg gtcgcatccg gtcgactcgt gcgagacctg
     1081 gagccgtccg agcctgtcga cctcgccgcc gttgtcgcag gtggtccccc tgagcaggtc
     1141 gccgtggcgc cgcgggcgac ggatgaggtg aaggcgcgcc gagagcgtcg cgatgctgag
     1201 cgcgtcgtgc gcgatgcgga gcaggagctg gctcgagcgc gatcggagca cgggaaggcg
     1261 gatcggaacc tgcacgacgc caccgcgaaa gccgaccggc tcagggcgcg cgtggtggag
     1321 ctggagtcgg aactgacccg gctgcgccac gaagccgaga tcgcgaccga aaccgtcgac
     1381 ggggccgccg acagggcggc ccgcgccgca gaacgcatgg cggaagccga gagagcgctc
     1441 gcagacgcac gagcagcgct ggacaggctc tagttcaggc gggtcctcga cgtcctatga
     1501 catctgatcg agaaccaggt cgccgcgcgc gttcaggagc tccatcatgt gctcgatcct
     1561 cttcgggtcg atcacgggca tggccgtgga gtcgaaggag aagatgagcg gaatcgcggg
     1621 gtgcatccac gtcgtgagcc gcccggactg cacggactgg tccgggagcc acgtcatcat
     1681 gaagctctcc tgacggcgca gcttggtacc gatgaccact ttcaggtgcg cgagcgtgat
     1741 gtcgtccacc tggatcggcg ggcgagagct gttgtactcg aggttcccca tgccagcaac
     1801 cgtacgccca gaatcgccgg ttcgatgccg acacgactcg gcggatgccg tcggtgcggc
     1861 gaggttgagc gatcgagggc tcggatgcaa gcccctcagc ggagaccgat cacgtcgcta
     1921 tcgtcgcggc catgactcag caaccgcgcg cactcatttc gatcgacggc aacgacggca
     1981 tcccgatcgc tccgggagag gacatcggcg acctgaagcg acgcatcgag gtcgctgccg
     2041 tcgagacgag gttcgtcgac ttcagcacag cagacggaac tcagctgagc gtgctggtct
     2101 cgccgtaccg gagggtgagc atcgccgtca tcgcgcctcg cgagcctcct gaccccacgc
     2161 tgctcgtcga cctcagccat ctgcctccgg atctgatgga cttctgaaca cgcggcgagg
     2221 aggatcaggc gtcgcgaaac ggctgtggcc atgcacgcgt gggcaaatgc ggacggagaa
     2281 tccagattga gtcgatgggg actccatcgc acgtgacgat cctcgccgac agggtggtca
     2341 agcgcggtcg atcaccggaa gcggcgacca acgaagcgga gcacgaccgt ggacggcggc
     2401 gcgatgtggt cagttggggg cagaaattgg gcgagggtcc aggcacccca ctcgtcgatt
     2461 tcgtccgacg aagagcgcgc tcgtcttggc cgacgcagcc cagtgcgaac cgtcacaagg
     2521 agaaccacgg aacggcgctc agtggctcac ggttgtcgtc cgcaaccggc ctgatggtga
     2581 ggacgtatcg tgggctgccg gcaacttggc tgagggtgac cagttgcgag ccttcctgta
     2641 cgggaagccg gatcgcgccc gcggccgtcg gcgcttcgtc gtcgaactgc gcagccatcg
     2701 tcagctgcgt gtagtccgtg agccacaacc ctccagctcc gacattgaca gcactgaccg
     2761 cttcacgcag ggctggagcg ccagggggat catctgccac ggtcacggcc tcgtccgcgt
     2821 cgtccgggcc gggataggcg acgaacaggg atccctcgcg catctgcccc acgaagtggg
     2881 tcatgagttg ctcgagctcc caatcctcgt cgacgaagcc ctgatatgcg tctggggcga
     2941 cgaggcagaa gaatccgtcc tcatccacat gcaagagaac ggcatcactc atggcagcac
     3001 cgtagctgag cgccatctcc gcgcgccaca tccgaggagg gattctcgtc gcccatcgat
     3061 gtgaactcga cctcgcccct cggatcagca gctggcaaga tccgcgccgg cggggcgtcg
     3121 agctcggtcg ggtctctcgt ctacgtgcgg gggcggtgag acgggatggg atcgggcttc
     3181 tctgcgcgcg atctcgcgtc acggatcgat gtctcgctcg ttggcctggc gccgtgccaa
     3241 tgctcacctc gcgccgcgtc ccattccgat ggcggggtgt gtttcgaatc cttgttctta
     3301 ctcatcttcg gactgtacgc gcccgacatg tgcgtgctca cagactcacg aagcgaagcc
     3361 cccaccggat acatccccgg ggcggacccg tccgccaaag cgcgacgacc acagtcgaag
     3421 ccacgacgac gcagccatca gggcaacatg tcgatgatgc gctgcagcat ctccttgtcc
     3481 atacgcactt caggggtgcg ctcgtcctcc accccatcgc gaccgaacat cagcgtcacg
     3541 gtctttcccc ggcccttgga tgccacggcg aagttcacca ggcgcagtgg cgacaccagc
     3601 tccttggtct cggactcgaa atcgaagtct tcgccgctat cgacagcgag cctgtggaag
     3661 tcgcacacga gagcctgcca aggccccgcc tgttcgggac tccgggtcac gaagatcggg
     3721 ttcgtcggtt tctcgctgca gcccttgaag gcacaggcag gcgtgtctgt catgagcctc
     3781 agtctgcgcc tgttcaccga cgcgatggtc cgaacacgac gaagagcgcc tctggatttc
     3841 ggctgccgtg gcgcggagag ccagtagggc ggctcctgca gcgcgcatac gtcgcccgaa
     3901 atctctgaga gcaagccggc ttggtgagtc ggcgacaagt tccggcccag aacgcagaaa
     3961 agccctggta gatggcgaga tctaccaggg cttgtccgtg cacccccagg gacttgaacc
     4021 ctgaacccat tgattaagag tcaattgctc tgccgattga gctagaggtg cgtgacccgg
     4081 aatgtcatcc ccggccgagg aattacatta ccagcgggga cgggtcgcgc caaatcacct
     4141 tcggatcccg ggcgcgttgc ccgcgatccg taccggatga cgtgtctgcg gcggatcgtt
     4201 tcacgaccga tacccggcac cccggtctgg cataccagcc cggactatcc tgttgaagtg
     4261 accgtccccc tcgcagccac agacttccat gccgacctcg acctcgccct tcgcctggcg
     4321 gatgccgccg atgagcaatc cctcccccgg ttcgatgcgg ccgacctcga ggtctcactg
     4381 aaggccgatc gctcgcacgt caccgatgcc gatctcgcca cggaacgcgc catccgcgac
     4441 atcctgcacg ccgaacgccc cgacgacgga atcctcggcg aggagttcgg cgcagaggga
     4501 gtcacgagcc ggcagtggat catcgacccg atcgacggga cggcgaactt cctgcgcggc
     4561 gtgccgctgt ggggcaccat gatcgctctg gcggtcgacg gcgtccccca ggtcggcgtg
     4621 gtcagcatgc ccgccctcgg gcgccgctgg tgggcatccg ccggcgacgg ctcctggacc
     4681 gccacgagcg acggagcccg cagactgtcc gtgtcgacgg tcgattcact cgacgaggcg
     4741 agcgtgagct tccagagcat ctcgcagtgg gcgcaggccg ggaagacccc cgaactgctg
     4801 gccctcgctg aacgcgtctg gcgcgaccgc gcctacggcg acatcttcag ctacatgctg
     4861 ctggccgaag gacgcatcga catggtcgcc gaattcgatg tcaaggagta cgacatcgcc
     4921 gcggcggttc cgatcgttcg cgaggccggc gggcggatga cggcgttcga tggcacggag
     4981 accctctccg cactgtccgc tctggcgacc aacggcatcc tgcacgactc gttcctttcc
     5041 ttgttccgcg acctgccgcg cgcctgagcg cgacaagcac ccctccccca cggatcacat
     5101 gaaaacccga ctccccctcg ctgccggcgc cctgctggcc gcgctgtcgc tcgccgcgtg
     5161 cagcggcccc agcacacccg tcgacaagac ccccacctcg accgcttcga agcccccggc
     5221 gacgccgaag gccgaggtgg agaccgctcc cgccgacaag cccgccacga ccacgcctgc
     5281 agaccggccg acctgcgaga cgatgatcac caagggcacc gtcgaggcgc tgaaatctca
     5341 gggctggacg ttcgaggagc gcgagttccg catcggcgac gtcctcgtcg acggcggact
     5401 cgagtgcctc tgggccgact actcgacggc atccgaccac ggtcagatgt acgcgtgggg
     5461 acccctcagc gccgagaact cccgcgcggc ccaggcgagc ctgatctcgg acggctggct
     5521 gcgctcgacc tcgaacggca agacgtacat caccgaggat cccgcctacg cgatcgccaa
     5581 ggacgacgac gggttcggca tgacctacga gttcggcgac gggtgggtga gattcgccga
     5641 caccaagcag ggactgctgc tcgtcgactg gggcgagaag tgaccaccgg acacagggag
     5701 agggcgccgc tgcatcagcg gcgccctctc ggcgtctgtg ggtcgacggc actgcagccg
     5761 tcgacgacga tcacttgtcg ccgcgctggt tccagttgtc gactcgacgg gtcgcggagc
     5821 cgcgctgccg catgacgtat gcgagggcga ggctgacgag gaggattccc gcggagaaga
     5881 ccacgccctg cgcggtcggc agctcccatg cgagcccgaa caggtagaac ccgccgagga
     5941 acaggaccat gaacacgatg aagtcgacca cgtcgaaccc tcccgatctt cggataacca
     6001 ctccagctta gtccggttgc gacccgttcc catgccggtc cgatgcgcgc gacggcgata
     6061 gattgggaat catgccgagg atgacaccgt gacgaccagc acgaggaccg cacgccgcgg
     6121 acggccgggt catgatcgcg ccgacgtcat ccgcgccggc gtcgagctct tcaacgagca
     6181 ggggtacgac gccacctcgg tctccgacct gacgggccgc ctcggcctga ccaagtcggc
     6241 gctgtaccat cacgtcgagt cgaaggagca gatccttcag atcgcactgg aggacgccct
     6301 gggcgggctc gagaaggcgc ttgaggatgc ccttcagcag cccacggccg gctcccggct
     6361 gacagccatc atcgagggtg ccgtgcgggt gctcacagag cgtcagcctc aggtcacgct
     6421 gctgctgcgg ctgcgaggca acagcgagat cgagacggcc gctctggccc ggcggcgccg
     6481 gttcgaccac accgtgaccg gcctggtccg cgaggcgcag tccgagggca tcgtgcgcac
     6541 cgatctggat gccggcgtcg cgacgcgcct gatcttcggc atgatcaact cggtcgtgga
     6601 gtggtaccgg cccgacggtc ctgtcgatcc ggaactgctg gcaggcgaga ttctcgcggt
     6661 ggcgctgggc gggcttcagc cgagagcctg atcagggcga gctgtcaccc cacacgcgga
     6721 cgacgccacg tgcggcctcg cggagcagtt cgccgtcgat cgtggtcgat ccctccagga
     6781 tgccgacggc atctgagacg tagtgaagcg tgacgtcgcc ttccgcgggc agcggccaga
     6841 gccaggcgcc gaccttcgag gtccagtggt cgctcgtccc gctcccgccg ccgtcgacca
     6901 tggtcagctg cggagactgc ggcggctcgc cgctcatgcg gcgcacgatc ccgcgaccgt
     6961 ccatcccgtt ctgctccgca ctcccctgag ccgtggtgac gccgagacgg aaaccgcgct
     7021 ccgctccggc ccgatgccgt ctcggatcga gcagggcttc gatctccgca cgacgctcag
     7081 ggctcaggtc gtcctccagg cggatgtcga gacgcagcac caccacgatc ccgtcgcgat
     7141 gggcgtcgat cctcttcaga tgaagagcgg cacccggcac gcgcgcgagc cggcgcgcca
     7201 cgggaaccgc gaccgggatc tcgtccgtcg gcggtcgcag ccagatcggt gtcggcggcg
     7261 cctgcatgcg ctcctgaggc ggttccggct ccagcggcgt gaagaacgac atggcgccag
     7321 tctgccgggt ccacgatccc gcgaacagcc cccagctgtt cacccccgcg actccgccct
     7381 atcgccgcac tcctgaacgc gtcgcaaaca tgtatataca actttctgat ctggcggtcg
     7441 gatggcactc ccacatccga tagtggaggc ggcggcatcc gccgtcatcc ccctcgcaga
     7501 aggatcgcca tgccctcgcc gaaccgcgct caccgcgttg cttcgaccat cctcctcacg
     7561 accgccatcg ccgcggtgtc cggcctcaca gccgccactg cggccgcttc gacccccgcg
     7621 gagagcaacc gcgacatcaa gaccctcgtc gtcggcatcg acggcgcgag cttcgacttc
     7681 ctcgagcccg cggggatgcc gaacctcgcg gcgctccggg cggacggact gaccgccacg
     7741 agcaacctgt acgcactgcc gatgtccggc acggtgtcag gcccgggctg gtcgacgatc
     7801 gcgaccggcg tgtggccgga caagcacaag gtcgtcgaca acagcttcag cacgccgaac
     7861 tacgccgagt accccgacta cctcactcgc atcgagcagg ccgcacccga gcgctccacc
     7921 ctggtggtcg gcacctggag tccgattccc cagaccgtgt tcggcccggc ggtcgacgag
     7981 cgcatcgccg gcggcgatga tgccggaacc accgcccgga ccgtggcggc actctccacg
     8041 agcgaccccg acgacgtctt cgtgcacctc gacgaggtcg acggcgcggg tcactcggtc
     8101 ggcacgaacg gccccgccta cggcgccgcg ctgcgtcgcg ctgacagcca gctcggtgag
     8161 atgctcgcgg cgatcgagtc ccgtccgacc cgagcttccg aggactggct ggtcatcgtg
     8221 accgccgacc acggccacac gcccaccgga ggccacggcg gtaacacacc cgccgagcgg
     8281 aagacgttcg tgatcgcctc cgggccgggg atcgtcgccg gctccgtgcg caacgacgtc
     8341 aagctcgtcg acatcgcccc gacggtgctc gcagccgacg gcatcgccac ggaccctgcg
     8401 tggaacatcg acggcagcgc gctcggcgat ctgaaggccg acgacttcga cagcgtccgc
     8461 ccggctctgc agccacagct cgatgagacg cgccccggga cgggagtgct cggctggacg
     8521 catcagacgc cggagggctg gacgatcgac aactcggcga tgcctgcggg cggcgtgcgg
     8581 gagtgggccg gctggtcatt cgcgactgac gacttctact cgaacgtcga acgcggacag
     8641 ggccgcgaga cgaacgtacg cagccgcgat gtgttcgcgg tggccgactc cgatgagtgg
     8701 gacgacaagg cgcatgacgc cggcgcattc gactccacgc tcgtgagccc cacctacccg
     8761 ctgacgggtg catccgatgc cgctgtgtcc ttcgcgacga cctacgtgat cgacggcccg
     8821 cagtccgccg aggtgctggt gagcttcgat ggcgccgagc cggtgtcgct gaagaactac
     8881 gcgaagaaca ccaacgcgaa cgaacggctc gagttcgcgg tgccgcaggg cgcgcagcag
     8941 gcgcagttcc gcttccgcta cacgggaacc aacagcgcgt tctggaccgt ggaccgcgtc
     9001 gccttccagc aggcgcagcc gactgcccct gcggctccgc gggcccttca ggtgacctcg
     9061 ggcgacgcca cggccgcagc atcctggcag gctccggcct ccgatggcca ttccccgatc
     9121 acggcctaca ccgtcacggc gacgcccatc gtcgacaagg gcaagtccaa gcaggcgact
     9181 cccctgtccg tgcggaccac cgcgctcaca gcgacgctga acggcctgcg caatggtgtt
     9241 tcctacacgg tgaccgtcac ggccacgaac gcctacggca cctccaccgc ctcggatgtc
     9301 gccgtagcga cccccacggt gtcccgcgcg acaccggcga ctcccccgtc gaagccgaac
     9361 aagggctgac ggcgtcgcac cgacgatgaa gaacccccgt cctcctgatg tggatcagga
     9421 cggcgggggt tcttcacgtg acaacggctt ggtggagctg gggggaatcg aacccccgtc
     9481 cgacgctgat catccacgtc ttctccgggc gcagtctgtg aaagcgttct gctcggctcc
     9541 gacctttgtc acagacacct aagtcgacga gcccagtctg ggaagagtcc cacgtgacgt
     9601 ccagacgccg tcacgcagca agatccctag atgacgccag ggtccgtatc gggatcacat
     9661 acggtctgac ggactatcgg gctcgcttag gcagcgaggg cgaagtcgtt gcgctttgca
     9721 ttggcaacta ttttttcgca gggggcgttc acgagataac cctgcatcct cggcccgctt
     9781 cccgtcgata cccagacgcc gtcgaaaccg atcagccccg tgggccttct tgcgaaggag
     9841 ccgctgtcac tctgtggaat taccagtcgg atgctgtcgc acccgagcac cacagactac
     9901 aacgtatccg cccctcgtgc cattccgcgc caccgaagcc gatcgcgtga cgtacagtcg
     9961 cactcatgag cgacgtcatc atcaccgtcc gcggcgagca cgagctgcgc atcgcgcccg
    10021 agcgcgccac cgtccatctg aacatccgcg ccgaggggcc gcagcgcgtc gccgcggtcg
    10081 acgaggtcac ccggctggcc gaacccgtac ggcagagcat cgtcgaccgc aaggacgcgg
    10141 agacggtcgt ggagtggtcg agcacccgga tgaccgtgca cgcagagcgt ccctggaacg
    10201 cggacggccg ccagctcgcg cccgtctacc atgccacgat cgcgttcgcc gcgaccttcc
    10261 cggatccctc cgagctctcg ctgtgggtct ccgacgtgtc cctgcgcgac ggcgtcgaag
    10321 tcggcaccgt ggactggcag ctcacgccgg agacgcggac gcgtctggaa cgggatgtcg
    10381 ccgctggcgc cgtttccgtg gccgtcgccc gggcggaggc ctacgccgcc gccctcggcc
    10441 tcgactccgt cgtgcccctc gaggtcgccg acctgggtct gatctcccgc caggagtccg
    10501 cccctcagcc caagatgctc atgcgcagcg ccgcgttcgc gatggattcg gccggggcct
    10561 ccggcatggt ctacgaagcg gaggacatcg tcgtctcggc gaccgtcgag gcgcgcttcc
    10621 aggccgcctg acggcccccg ggcgtcggcg tccggctcag cccaccgcgt agggcgcgag
    10681 ctcagccgcg agccgctccg acacgtgcac gtgcaccatg gtgcccgcct cttcgtgctc
    10741 agccgagatc agcaccccgg tctcgtgcac ggccgagacc aggtcgccgc ggtcgtaggg
    10801 cacgagcgcc cgcacctcga cggccggcat cggcagcatc cgctcgatcg cctcgcgcag
    10861 ctcatcgatg ccctcgcctg tgcgcgagga gacgaagtgc gcgccgggcg ccagcccgcg
    10921 cagcacgaga cgctcgtcct cgtcgaccag atcggccttg ttgaacacga cgatctcggg
    10981 gatgctgcgc gcgccgacgt cgcccatcac gtcgcgcacc gtctgcagct gcgcggacgg
    11041 atccggatgc gaaccgtcaa cgacgtgcac gatcaggtcg gcctgtccga cctcctcgag
    11101 cgtggagcgg aacgcctcga cgagctgatg cggcaggttg cgcacgaagc cgaccgtgtc
    11161 ggtgagcgtg tagatgcgtc cgtcgtgggt ctcggcgcga cgcacggtgg cgtccagggt
    11221 cgcgaacagc gcgttctcga ccagcacacc ggcgctggtc agcgcgttca gcagactcga
    11281 cttgccggcg ttcgtgtacc cggcgatcgc gaccgacggg atcgtgttgc gcttgcgctc
    11341 ggcgcgcttg gcgtcacgcg cgggcgcgaa ctcgcggatc tgcttgcgca gctgcgccat
    11401 gcgagtgcgg atgcggcggc gatccagctc gatcttggtc tcaccggggc ctcgcgagcc
    11461 catgcccgct cccccggcgc cgacctggcc accggcctgg cgactcatcg actcgcccca
    11521 gcctcgcaga cgagggagca ggtactcgag ctgcgcgagc tcgacctgcg ccttgccctc
    11581 acggctcttg gcgtgctggc tgaagatgtc caggatgacc gtggtgcggt cgatgacctt
    11641 gaccttcacg acatcctcca gcgcgcggcg ctggctggcg gcgagctcgg tgtctgcgat
    11701 gacggtgtcg gcgccgacgg ccgccacgat gtccttcagc tcctgcgcct tgcctcggcc
    11761 gaggtaggtg gcggggtccg gatgcgggcg gcgctgcagc agcccgtcga gcacggcg
//
LOCUS       DATMSX010000163         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_172875,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000163 DATMSX010000000
VERSION     DATMSX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172875"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VN041_05025"
     CDS             <1..708
                     /locus_tag="VN041_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU28441.1"
                     /translation="LGAAIATALHDAGAEVIVVGRNEARLEQVVDRLGDRARALTCDV
                     ADAASVDALRDALSGTEISILVNNAGIPGPVAALTDIDPGEWDEVFDVNVRGTFLLCR
                     AFLPGMVERGEGDVINVASVSGKRPLAHRTPYCASKMAVIGLTSTLAFEVGPSGVRVN
                     TLSPGPVAGPRMERNFRLEAERSGASIADAEQAFVSRTALGRMVTETEVGAAVVAMLG
                     MPGMCGADVDLSAGMVA"
     gene            708..1511
                     /locus_tag="VN041_05030"
     CDS             708..1511
                     /locus_tag="VN041_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWU28442.1"
                     /translation="MAISLRQRALRGDRLIGVLLRMPSEELVEMSAVAGMDFVLVDCE
                     HGPADVIALRHHIALASVHGVPVIVRVGENDPGQILRVLDQGAQGVLVPHIDSREEAD
                     AMVASSLYPPDGTRGFATYSRAGRFGTVPAAEHRAWFLENTLVLGMIESPAAVAAVRE
                     IAGAHRLDGLMIGPSDLAAASGPADPSLSEAAATVDAALRAEGRLRMDIVGDAAAAQV
                     AFSDGAHLVVYNLAHSLMSNLERLVEGRPWPRRTPTGPTPTQRARSSKG"
BASE COUNT          201 a          571 c          583 g          242 t
ORIGIN      
        1 ctcggcgccg cgatcgccac ggcgctgcat gacgccggcg ccgaggtgat cgtggtcggc
       61 cggaacgagg ccaggctgga acaggtcgtc gaccggctcg gcgatcgcgc ccgcgcactc
      121 acctgcgacg tggcggatgc cgcatccgtc gacgccctgc gggacgccct cagcgggacc
      181 gagatctcga tcctggtcaa caacgccggc atccccgggc ccgtcgcggc gctcaccgac
      241 atcgaccccg gcgagtggga cgaggtgttc gacgtgaacg tgcgcggcac gttcctgctc
      301 tgccgggcgt tcctgcccgg catggtcgag cgcggcgagg gggacgtgat caacgtggca
      361 tccgtctcgg gcaagcgccc gctcgctcac cgcaccccgt actgcgcatc gaagatggcc
      421 gtgatcgggt tgacctcgac gctcgcgttc gaggtcgggc cgtcgggcgt gcgggtcaac
      481 accctctctc ccgggccggt cgccggcccg aggatggagc gcaacttccg cctcgaggcg
      541 gagcgctccg gggcgtcgat cgcggatgcc gagcaggctt tcgtgtcccg gaccgccctg
      601 ggcaggatgg tcacggagac cgaggtcggc gccgccgtcg tcgcgatgct ggggatgccc
      661 ggcatgtgcg gcgcggacgt cgatctgtca gcggggatgg tggcgtgatg gcgatctccc
      721 tgcgacagcg cgccctgcgc ggcgatcgcc tgatcggcgt gctgctgcgg atgccgtccg
      781 aggagctcgt ggagatgtcg gcggtcgccg gcatggactt cgtgctcgtc gactgcgagc
      841 acggtcccgc cgatgtgatc gcgctgcgcc accacatcgc cctcgcctcg gtgcatggcg
      901 ttcccgtgat cgtgcgcgtc ggggagaacg acccggggca gatcctgcgc gtgctcgatc
      961 agggcgcgca gggggtgctg gtgccgcaca tcgactcccg tgaggaggcg gatgccatgg
     1021 tcgcctcctc actctacccg ccggacggca cgcgcggatt cgcgacctac agccgggccg
     1081 gtcgtttcgg gaccgtgccg gcggccgagc atcgtgcctg gttcctggag aacacgctcg
     1141 tgctcggcat gatcgagtcg cccgcggccg tcgccgccgt gcgcgagatc gccggcgccc
     1201 accgcctgga cggcctgatg atcggcccgt ccgatctggc cgcagcatcc ggccccgccg
     1261 atccctcgct gtccgaggcg gccgcgacag tggacgcggc gctgcgagcg gagggccggc
     1321 tgcggatgga catcgtcggc gatgccgctg ccgcgcaggt ggcgttctcg gacggcgccc
     1381 acctggtcgt ctacaacctg gcccattcgc tcatgagcaa cctcgagcgc ctggtcgagg
     1441 gccggccctg gccgcggcgc acgcccaccg gcccgactcc gacgcaacgg gctcggtcgt
     1501 cgaagggctg agacccgtcg cccggatgcc ccgtcagatc cggctctgcc tgcgccagtt
     1561 gcgcaccagc gagccgatct cggcggggcg ttcgagc
//
LOCUS       DATMSX010000164         5693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_153887,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000164 DATMSX010000000
VERSION     DATMSX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153887"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /locus_tag="VN041_05035"
     CDS             <1..2065
                     /locus_tag="VN041_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HWU28443.1"
                     /translation="GRGTPGPDAPWGMRGTQSVRWRESDGIRGLDFDIELDHDDPGYG
                     TMSLTTTDSSTTVKPQWVTSYWPDGARLFWNDLADDGLLAPEPRLTLEDKPRGLFAER
                     DADPDAPALTEEQMLAKLPRVRTGSLGIVHTLAPGEERDFEFVLAWSFPNRRRGWHGH
                     IIFDDALEDGTPDLRDELSPIVRNHYSALWPDAWAAAAQLHRDLPALEGATDAFVEEL
                     YGGSLDPVLADAVGANIAALRSTTCYVLESPTPELGDGPVFAAWEGSFDHGGSCEGTC
                     THVWSYAQTAAWLFPGLERSARRAEYLLETDESGAQKFRGNRIFGAPRWFIGPAVDGQ
                     LGTFLRLHRDWRFCGDDEFLRELWPAAARTLDYAAREWDHDGDGLLDGEMHNTYDIEF
                     HGVEPLSNIIHLAALRAGVRMAEHLGDTARAQEWALRADHVAAAIESVLWNGQYYRQV
                     IDDVDAHRYQYGDGVLSDQLLGQFHAFLGGLGYLLPEAHVRSALDAIVQHNHRSDLRD
                     HESTQRVYALNDEGGLLLASWPEGGRPALPFVYADEVWTGIEHQVAVSLLFAGRYDDA
                     LRIERALRARYDGAHRNPWNEIECGNHYARSLASWGLLIGASGAQWDAGVRTLSFDPV
                     LPGDARFLFTTATGWGGVEIGDDAITLRLHGGALDLDELRLRGEVAGRGIHLDAGETR
                     TLTLT"
     gene            2092..3723
                     /locus_tag="VN041_05040"
     CDS             2092..3723
                     /locus_tag="VN041_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HWU28444.1"
                     /translation="MSYTLPTPVARPEAAPKTAYLITSGDLRESANVAGWPTQVELET
                     GVTGVLEGLGWTVIRPFGVDPATGHGFISSQRMGMEVFKSIPVDAPLVVAIANWQYSH
                     HVLAGLRTHQGPILTVANFAGDWPGLVGLLGLNAGLTKMGKEYATTWSVDFTDDWFTG
                     GIREWTETGRITHDASHVHALPELPDSAEKQLGQALAAELLADKAIIGVFDEGCMGMY
                     NAIFDDELLNTIGIYKERLSQSALYAEMLEVTEAEADAAYDWLVDHGMTFKYGEDAAT
                     ELTREQVQWQMKMYIAALRIADDFGLDAVGIQYQQGLKDLVPASDLAEGILNSTERPP
                     VTSRDGSRVLHEGRAFPHFNEADEGVAVDALITDRVWRAMGLVPDNTLHDVRWGEEHD
                     GEFVWVYEISGSVPASHLGGWDKAEGWRQGHVFFPAGGATINGVSKPGEVVLSRVFIA
                     DGILQADIFRASVVELPEAETQRRKDATNPEWPIAHVVLHGQSRDQFMARHKANHAQL
                     VYAPDAETADKALIAKAAMFDGLGIKVNLVGDVTI"
     gene            complement(3799..4365)
                     /locus_tag="VN041_05045"
     CDS             complement(3799..4365)
                     /locus_tag="VN041_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28445.1"
                     /translation="MAKNEARRNALADAGITVLAREGSRGLTHRAIDAEAGVPLGTTS
                     NYFRSREKLIAGLVERIGIRLSPTPEDLAARASDAPTRELFADYVRDIVRRLTTHRDV
                     TLALFELRLEVARRPDLAAAMREWQHQALDGDVAFNEAAGLPGGRREITLFHYAIDGL
                     VFDRLTSPIDPGTSTDDIVDDLVAGLLR"
     gene            4437..5009
                     /locus_tag="VN041_05050"
     CDS             4437..5009
                     /locus_tag="VN041_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU28446.1"
                     /translation="MRELTYFVAVSVDGFIAGPNGEFDRFLAEGDHMDVIGEKYRGTV
                     PTVLADRAGVPADGSPFDTVVMGWNTYAVGLPMLPDPYSHLRQVVFTRSPDAPEGAEA
                     VEFTDRDPVDVVRELKAEEGRGIWLCGGGSLAGALIDEIDHLVLKISPVLLGDGIRLF
                     GDSSYAPTGMMPDAVTRFDSGVVIAEYSRG"
     gene            complement(5011..>5693)
                     /locus_tag="VN041_05055"
     CDS             complement(5011..>5693)
                     /locus_tag="VN041_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28447.1"
                     /translation="QAYPSRLNRRGGRAARDARSQRIPRDRPYGGGMRLISSADPSLW
                     IPVTGSLGFTGRRHRKAAIRMLMAQANSAMGRDDRPGSRVGAWLASQAIPLAMRKADG
                     RVLAWTWKAEPELVVAMANVQELTPQLRTMRAMMPIEYDDTESFPTPLGVGEKLLMPM
                     PANPASPPSATYTWDTGTHLITLTAVCSDRERFGTLEKQLDDLARSIRIAADLTGGES
                     NILQLPPA"
BASE COUNT          866 a         2032 c         1942 g          853 t
ORIGIN      
        1 cggccggggc acgccggggc cggacgcgcc ctggggcatg cgcggcacgc agtcggtgcg
       61 ctggcgcgag agcgacggca tccgcggtct cgacttcgac atcgaactcg atcacgacga
      121 ccccggctac gggaccatga gcctgacgac gaccgactcg tcgacgaccg tcaagccgca
      181 gtgggtcacc agttactggc ccgacggtgc gcggctgttc tggaatgacc tcgccgatga
      241 cggcctgctc gcgcccgaac cgcgactgac gctggaggac aagccccgag ggctgttcgc
      301 cgagagggat gccgatccgg acgcgcctgc gctcaccgag gagcagatgc tcgccaagct
      361 gccccgcgtg cgcaccggat cgctcggcat cgtgcacacg ctcgcgccgg gggaggagcg
      421 cgacttcgag ttcgtgctgg cctggagctt ccccaaccgc cggcggggat ggcacgggca
      481 catcattttt gatgacgctc tggaagacgg gacgcccgac ctgcgcgacg agctgagccc
      541 catcgtgcgc aaccactact cggcgctgtg gccggatgcc tgggccgcgg cggcgcagct
      601 gcaccgggac ctgcccgctc tggagggcgc gaccgacgcc ttcgtcgagg agctctacgg
      661 cgggtccctc gatcccgtgc tcgccgatgc ggtcggcgcc aacatcgccg cgctccgctc
      721 caccacctgc tacgtgctgg aatcgcccac ccccgagctc ggcgacggac cggtcttcgc
      781 cgcctgggag gggtcgttcg accacggcgg ctcctgcgag ggcacctgca cccacgtctg
      841 gtcgtacgcg cagaccgcgg cctggctgtt ccctggcctg gagcgcagcg cccgccgcgc
      901 ggagtacctg ctcgagaccg atgagagcgg agcgcagaag ttccgcggca acaggatttt
      961 cggggctccc cgctggttca tcggccccgc cgtcgacgga cagctcggca ccttcctgcg
     1021 cctgcaccgc gactggcgct tctgcggcga tgacgagttc ctccgggagc tgtggcccgc
     1081 cgcggcgcgc accctcgact acgccgcccg cgaatgggat cacgacggcg acgggctgct
     1141 cgacggcgag atgcacaaca cctacgacat cgagttccat ggtgtcgagc cgctctcgaa
     1201 catcatccac ctcgccgcgc tgcgcgccgg cgtccgcatg gcggagcatc tcggcgacac
     1261 ggctcgcgcg caggaatggg ccctgcgggc cgaccatgtc gcggccgcga tcgagagcgt
     1321 cctctggaac ggccagtact accggcaggt gatcgacgac gtcgacgccc accgctacca
     1381 gtacggcgac ggcgtgctgt cggatcagct gctcggccag ttccatgcct tcctgggcgg
     1441 tctcgggtac ctgctgcccg aagctcatgt gcgctccgcg ctggacgcga tcgtccagca
     1501 caaccaccgc agcgacctgc gcgatcacga gagcacccag agggtgtacg cgttgaacga
     1561 cgagggcggt ctgctgctgg catcctggcc cgaaggaggg cgccccgccc tgccattcgt
     1621 ctacgcggac gaggtgtgga cgggcatcga gcaccaggtc gcagtctcgc tgctgttcgc
     1681 cgggcggtac gacgacgcgc tgcgcatcga gcgcgcgctg cgggcccgct acgacggggc
     1741 gcaccgcaac ccgtggaacg agatcgagtg cggcaaccac tacgcccgct cgctcgcatc
     1801 ctgggggctg ctgatcggcg ccagcggcgc gcagtgggat gccggcgtgc gcactctctc
     1861 gttcgatccg gtgctgccgg gggacgcccg cttcctgttc accaccgcaa ccggatgggg
     1921 cggcgtcgag atcggtgacg acgcgatcac cctgcggctc cacggcggcg cgctcgacct
     1981 cgacgagctg cggctccgcg gcgaggtcgc cggtcgcggc atccatctcg atgcgggcga
     2041 gacccgcacc ctgactctca cctgaacctc acacccacaa cggagaatcc catgagctac
     2101 accctcccca ccccggtcgc ccgccccgag gcggcgccga agaccgccta cctgatcacc
     2161 tccggcgacc tgcgcgagtc cgcgaacgtc gccggctggc ccacccaggt cgagctcgag
     2221 accggtgtga ccggcgtgct cgagggcctc ggctggacgg tcatccggcc cttcggcgtc
     2281 gaccccgcca ccggacacgg cttcatctcc agtcagcgca tgggcatgga ggtgttcaag
     2341 agcatcccgg tcgacgcccc gctcgtcgtc gccatcgcga actggcagta ctcgcaccac
     2401 gtgctcgcag gtctgcgcac ccaccagggc ccgatcctca ccgtcgcgaa cttcgccggc
     2461 gactggcccg gcctggtcgg actgctcgga ctgaacgcgg gcctcaccaa gatgggcaag
     2521 gagtacgcca ccacctggtc ggtggacttc accgacgact ggttcaccgg cggcatccgt
     2581 gaatggaccg agaccggccg catcacgcac gacgcctcgc acgtgcacgc actgcccgag
     2641 cttccggact ccgcggagaa gcagctgggt caggcgctcg ccgccgagct gctcgccgac
     2701 aaggccatca tcggcgtgtt cgacgagggc tgcatgggca tgtacaacgc gatcttcgac
     2761 gatgagctgc tcaacaccat cggcatctac aaggagcgcc tgtcgcagtc ggcgctgtac
     2821 gccgagatgc tcgaggtgac cgaggctgag gccgacgccg cctacgactg gctggtcgac
     2881 cacggcatga cgttcaagta cggcgaggat gccgccaccg agctcacccg cgagcaggtg
     2941 cagtggcaga tgaagatgta catcgccgcc ctccgcatcg ccgacgactt cgggctcgac
     3001 gcggtcggca tccagtacca gcagggtctg aaggacctcg tgcccgcctc cgacctcgcc
     3061 gagggcatcc tgaactccac cgagcgcccc ccggtgacct cgcgcgacgg ctcccgcgtg
     3121 ctgcacgagg gccgcgcctt cccgcacttc aacgaggccg acgagggcgt ggcggtcgat
     3181 gcgctgatca ccgaccgggt gtggcgggcg atgggtctgg tgccggacaa caccctgcac
     3241 gacgtgcgct ggggcgagga gcacgacggc gagttcgtct gggtctacga gatctcaggc
     3301 tccgtgcccg cctcgcacct gggcggctgg gacaaggccg agggctggcg ccagggccac
     3361 gtgttcttcc ccgcgggcgg cgccaccatc aacggcgtct ccaagcccgg cgaggtcgtg
     3421 ctctcgcggg tgttcatcgc cgacggcatc ctgcaggccg acatcttccg ggcatccgtc
     3481 gtggagctcc ccgaggccga gacgcagcgc cgcaaggacg cgaccaaccc ggagtggccg
     3541 atcgcccacg tcgtgctgca cggccagagc cgcgaccagt tcatggcccg ccacaaggcg
     3601 aaccacgcgc agctggtcta cgcgcccgac gccgagacgg ccgacaaggc gctgatcgcg
     3661 aaggccgcca tgttcgacgg cctggggatc aaggtcaacc tggtgggcga cgtcacgatc
     3721 tgatccgcgt catcctcgat cgagaacccg cctcccgcgt ccgcgcaggg ggtgggttct
     3781 tcgtcgtaag cgcttcggtc agcgcagcag ccccgcgacc aggtcgtcga cgatgtcgtc
     3841 agtggaggtg ccggggtcga tgggggaggt gaggcggtcg aacaccaggc cgtcgatcgc
     3901 atagtggaac agggtgatct cgcggcgccc tccgggaagc ccggcggcct cgttgaaggc
     3961 gacgtcgccg tccagcgcct gatgctgcca ctcccgcatg gccgcggcca gatcgggccg
     4021 ccgcgccacc tcgagccgca gctcgaacag tgccagggtc acgtcgcggt gcgtggtgag
     4081 ccgtcggacg atgtcgcgca cgtagtcggc gaacagctcg cgggtcgggg cgtcggatgc
     4141 ccgtgctgcg aggtcctccg gagtggggga gaggcggatg ccgatgcgct cgacgagccc
     4201 ggcgatgagc ttctcgcgac tgcggaaata gttcgatgtc gtgccgagtg gcacaccggc
     4261 ctcggcatcg atcgcgcggt gcgtgagtcc ccgtgagccc tccctggcca gtacggtgat
     4321 gcccgcgtcg gccagcgcgt tgcgtcgtgc ctcgttcttc gccatgatcc gagactagcg
     4381 caaccacttc acttgttgta gtctcaaacc actgcacatg tcgtgattgg aatcagatgc
     4441 gagaactcac ctacttcgtc gccgtctccg tggacggctt catcgccgga ccgaacggag
     4501 agttcgaccg gttcctcgcc gagggcgacc acatggacgt catcggcgag aagtaccgcg
     4561 gcaccgttcc gacggtgctc gccgaccgcg ccggcgtgcc cgccgacggc agccccttcg
     4621 acaccgtcgt gatgggctgg aacacctacg ccgtcggcct gcccatgctg ccggacccgt
     4681 actcccacct gaggcaggtg gtcttcaccc gctcgcctga cgccccggag ggcgccgagg
     4741 ccgtcgagtt caccgaccgc gaccccgtcg acgtcgtgcg cgagctgaag gccgaggaag
     4801 gccgcgggat ctggctctgc ggcggcggat cgctcgccgg cgcactcatc gacgagatcg
     4861 accacctcgt gctgaagatc agccccgtgc tgctcggcga cggcatccgg ctgttcggcg
     4921 acagctccta cgccccgacc ggaatgatgc ccgacgcggt gacccgtttc gactcgggcg
     4981 tcgtgatcgc ggagtactcc cggggatgag tcaggcgggc gggagctgca ggatgttcga
     5041 ctcgccgccg gtcaggtcgg cagcgatgcg gatgctgcgg gccaggtcgt cgagctgctt
     5101 ctccagcgtg ccgaaccgct cgcggtcgct gcacaccgcg gtgagcgtga tcagatgcgt
     5161 gccggtgtcc caggtgtagg tcgccgacgg cggcgacgcc gggttcgcgg gcatcggcat
     5221 gagcagcttc tcgcccaccc cgagcggggt cgggaagctc tcggtgtcgt cgtactcgat
     5281 cggcatcatc gcccgcatgg tgcgcagctg aggggtgagc tcctgcacgt tcgccatcgc
     5341 gacgaccagc tcgggctcgg ccttccacgt ccaggcgagc acgcggccgt cggccttgcg
     5401 catcgccagc ggaatcgcct ggctcgccag ccacgcgccc acgcgcgatc ccggacggtc
     5461 gtcgcgtccc atcgcactgt tggcctgcgc catcagcatc cggatcgcgg ccttgcggtg
     5521 ccgccgaccg gtgaagccga gcgaaccggt gacggggatc cacagcgacg ggtcggcgga
     5581 ggagatcagg cgcatgccac caccgtaggg ccggtcgcgg gggatgcgct gtgagcgggc
     5641 gtcgcgtgcg gcccggcctc cccgccggtt cagcctgctc gggtaggctt gcc
//
LOCUS       DATMSX010000165         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_125774,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000165 DATMSX010000000
VERSION     DATMSX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125774"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VN041_05060"
     CDS             complement(<1..178)
                     /locus_tag="VN041_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU28448.1"
                     /translation="MIPQDRHVPRRVLLVRHGQTYWNVEGRLHGQLDSGLTAEGIAQA
                     TAVAERLVPFGARTV"
     gene            complement(218..1000)
                     /gene="map"
                     /locus_tag="VN041_05065"
     CDS             complement(218..1000)
                     /gene="map"
                     /locus_tag="VN041_05065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWU28449.1"
                     /translation="MIELRTPAEIDEMRAAGRFVAETLATLRAETKVGTNLLAIDRHA
                     HDLIRKAGAESCYIDYAPSFGNGPFGKVICTSINDAVLHGLPHDYTLRDGDLVTLDFA
                     VSVDGWVADSAVSFVVGTPRDEDLRLIDTTERALDAAIEVATVGNRIGDISAAIAAIS
                     HGEGYSINTDFGGHGVGRVMHGDPHIPNDGRAGRGFPLRAGLVFAIEPWLLATTDEVV
                     TDPDGWTLRSVDGSRGAHSEHTIAVTEDGPIILTDRSFLGVN"
     gene            1105..1593
                     /locus_tag="VN041_05070"
     CDS             1105..1593
                     /locus_tag="VN041_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter permease"
                     /protein_id="HWU28450.1"
                     /translation="MWLREAFFRWLVPAAFLLPLWLFVGWAIFGQNAWSLLWVLLMAM
                     PTVFVGQLILTLLTRSRPSVRAERAVSWWDVAGFTVWHVLTIAVGLFVDGGFGWLLTG
                     AILAAIALFWLQLWQLWNEARGTGIRIRETVSWSTAPRVDDSASQPAPDVIVIEESRT
                     KD"
     gene            1764..2111
                     /gene="rplS"
                     /locus_tag="VN041_05075"
     CDS             1764..2111
                     /gene="rplS"
                     /locus_tag="VN041_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWU28451.1"
                     /translation="MQILDAVDAASLRSDIPIFHPGDTVKVHVNITEGSRSRIQVFQG
                     VVIGRQGDGVRETFTVRKISFQVGVERTFPVHSPVIDHIEVVTRGDVRRAKLYYLRNL
                     RGKKAKIKEKRDN"
     gene            2232..2966
                     /gene="lepB"
                     /locus_tag="VN041_05080"
     CDS             2232..2966
                     /gene="lepB"
                     /locus_tag="VN041_05080"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWU28452.1"
                     /translation="MTSDAAESAEKPTRRRGPWVFLRDVLVIIIIAALVSFVVKTFVV
                     RSFYIPSGSMERTLLINDRILVDELTPRWSGYDHGDVVVFKDPGGWLPPAPQTPARPF
                     LVEAADWVLTFVGLSTTDAQDHLVKRVIGLPGDHVVCCNALGQITINGSPIDELSYLN
                     LPGGDTAASDVDFDVTVPANSLWVMGDNRDRSQDSRAHQDLPGGGFVPLGDVVGRAFL
                     TTWPLDRFGLIDTHDEVFRSIPEPKK"
     gene            2963..>3128
                     /locus_tag="VN041_05085"
     CDS             2963..>3128
                     /locus_tag="VN041_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWU28453.1"
                     /translation="MTVVAPTLRLERRLLREEEFELIIALDEVGRGALAGPVAVGAAV
                     MDAAGSRRRVP"
BASE COUNT          479 a         1041 c         1052 g          556 t
ORIGIN      
        1 acaccgtccg cgctccgaac gggacgagtc gctccgccac ggcggtcgcc tgtgcgatgc
       61 cctcagcggt gagaccggag tcgagctggc cgtgcagtcg gccttcgacg ttccagtacg
      121 tctgaccgtg acgcaccagc agtacccgcc gcggcacgtg acggtcttga gggatcatgc
      181 cctcagagtg gcatataccg gcgtgaaacc cgagtgctca gttcaccccg aggaacgagc
      241 ggtcggtgag gatgatcggg ccgtcctcgg tgaccgcgat cgtgtgctcg gagtgagcgc
      301 cgcgtgagcc gtcgacgctg cgcagcgtcc atccgtccgg gtcggtgacg acctcatcgg
      361 tggtggcgag cagccacggt tcgatggcga acacgagacc cgcacgcaac gggaatccgc
      421 gtcctgcgcg cccgtcgttc ggaatgtgcg ggtcgccgtg catgacgcgg ccgacgccgt
      481 gcccgccgaa gtcggtgttg atggaatagc cctcgccgtg cgagatcgcg gcgatcgccg
      541 ccgagatgtc accgatgcgg ttgccgacag tggcgacctc gatcgcggca tccagcgccc
      601 gctcggtggt gtcgatgaga cggagatcct catcgcgcgg cgtgcccacc acgaacgaca
      661 ccgccgagtc ggcgacccag ccgtcgaccg agaccgcgaa atccaacgtc acgaggtcgc
      721 cgtcgcgcag cgtgtagtca tgggggagtc cgtgcagcac cgcgtcgttg atcgaggtgc
      781 agatgacctt gccgaacggg ccgttgccga acgagggggc gtagtcgatg tagcaggact
      841 cggctccggc cttgcggatc agatcgtgtg cgtgacggtc gatggccagc aggttggtgc
      901 ccaccttcgt ctccgcgcgc agcgtcgcca gtgtctccgc gacgaagcgg ccggcggcgc
      961 gcatctcgtc gatttcggca ggtgtgcgca gttcgatcat gggaaggatc ctctcgtcag
     1021 cttccattct ccccgatccg cggacgcagc aagctcacag cgaacacctg ccgctggata
     1081 cgggcgggtg cgtacgatcg gagcatgtgg ttgcgtgagg cgttcttccg ctggctcgtg
     1141 ccggcggcct tcctcctgcc gctgtggctc ttcgtgggct gggcgatctt cggccagaac
     1201 gcgtggagtc tgctgtgggt gctgctgatg gcgatgccga cggtcttcgt cgggcagctg
     1261 atcctcaccc tgctcacccg ctcgcgtccc tcggtgcggg cagagcgcgc ggtgtcctgg
     1321 tgggacgtcg ccggcttcac ggtctggcat gtactcacga tcgccgtcgg cctgttcgtg
     1381 gacggcggct tcggatggct cttgaccggc gcgatcctcg ccgcgatcgc gctgttctgg
     1441 ctgcagctct ggcagctctg gaacgaggcg aggggaacgg gcatccgcat ccgcgagacc
     1501 gtcagctgga gcaccgcacc gcgcgtcgac gactcggcat ctcagcccgc ccctgacgtg
     1561 atcgtgatcg aggagagccg cacgaaggac tgagccggcc ggttttgagc gggctccggt
     1621 tcatggcaga atagatgttt gtgccgtgaa ccggttctgc ctcaggggaa cccgcggacg
     1681 cctcgcgcgc cgctcggcac acacccgttc ttattccttt gaatcatgca tccgacctgt
     1741 ggcgagtgca gagagagacg atcatgcaga tcctcgacgc cgtcgacgcg gcttcgctcc
     1801 gttccgacat ccctatcttc caccccggtg acaccgtcaa ggtgcacgtg aacatcaccg
     1861 agggttcgcg ttcgcgtatc caggtcttcc agggcgtcgt catcggccgc cagggcgatg
     1921 gtgtgcgcga gacgttcacc gttcgcaaga tcagcttcca ggtgggcgtg gagcgcacct
     1981 tcccggtgca ctcgccggtg atcgaccaca tcgaggtcgt cacccgcggt gacgtgcgtc
     2041 gcgccaagct gtactacctg cgcaacctgc gcggcaagaa ggcgaagatc aaggagaagc
     2101 gcgacaactg acgcgcagct cttctgcaga accccgagac actcccgtct cggggttctg
     2161 tcgtctgcac gtccgtgtgc gaaccttgta gatgccgccg tcgtgcggcg aatcagacga
     2221 agagagtcca tatgacatcc gacgccgccg aatccgcgga gaagccgacg cggcggcgag
     2281 gaccctgggt cttccttcgc gacgtgctcg tgatcatcat catcgccgcg ctggtgtcgt
     2341 tcgtcgtcaa gaccttcgtg gtgcgctcgt tctacatccc ctccgggtcg atggagcgca
     2401 cgctgctgat caacgaccgg atcctcgtcg acgagctgac cccgcgctgg agcggctacg
     2461 accacggcga cgtggtggtg ttcaaggatc cgggtggatg gctcccgccg gccccgcaga
     2521 cgccggcgcg cccgttcctc gtcgaggcgg cggactgggt gctcaccttc gtgggcctgt
     2581 cgaccaccga cgcacaggac cacctcgtca agcgcgtcat cggcctcccg ggcgatcacg
     2641 tggtgtgctg caacgcactc ggccagatca cgatcaacgg ctcaccgatc gacgaactgt
     2701 cctacctcaa cctccccggg ggcgacaccg cggcatccga cgtcgatttc gacgtcaccg
     2761 tgccggcgaa ctcgctgtgg gtgatgggcg acaaccgcga ccgctcgcag gactcccgcg
     2821 cgcaccagga tcttcccggt ggcgggttcg tgccgctcgg cgatgtcgtg ggccgtgcgt
     2881 tcctgacgac gtggccgctg gaccgcttcg gcctcatcga cacccacgat gaggtgttcc
     2941 gctcgatccc ggaaccgaag aagtgaccgt cgtcgcgccc accctccggc tggagcgccg
     3001 gctgctgcgc gaggaggagt tcgagctgat catcgcgctc gacgaggtcg ggcgcggagc
     3061 gctggcgggt cccgtcgcgg tcggcgcggc ggtgatggat gccgctggct cgcgccggcg
     3121 cgttcccg
//
LOCUS       DATMSX010000166         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_97324,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000166 DATMSX010000000
VERSION     DATMSX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97324"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN041_05090"
     CDS             complement(<1..331)
                     /locus_tag="VN041_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HWU28454.1"
                     /translation="MQRVIVLLLAALDAAIAAAIGLAAVLAPFTLLWVLSFGVDADWG
                     ALWPVSGTLWQFGHGVPLQVVLPDAVLSGLAIPKEAATFALSVPPLAILLFTVIFAAR
                     SGRRAAIA"
     gene            422..1867
                     /locus_tag="VN041_05095"
     CDS             422..1867
                     /locus_tag="VN041_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWU28455.1"
                     /translation="MTTAPAHPDSDTEPRNALDRFFEITKRGSSFGAEIRGGVVTFVT
                     MAYIVILNPIILSTTADIDDHKLGFLQLSAVTGLTAAVMTILFGLIARLPFGFAAGLG
                     INAFLAFSVVGEVTWPEAMGLVVINGLIIVLLSATGLRRMIFDAVPMQLKIAITVGIG
                     LFIAFIGFVNAGFVTAAGSPPVTLGIGGSVSTVPTVIFVFTLLLTGILVARKVKGGIL
                     IGLVSGTVLAVIVEAIWHIGAKGEKNPGGWGLSVPALPKELVSLPDLSLVGQVDLFGA
                     FGRIGALAALMLVFTLVFTNFFDAMGTMTGLAKESGLADEKGNFPRLKSALIVEGVGA
                     VVGGATSGSSNTVFIESGAGIGEGARTGLANIVTGALFLIAMFFTPLVSIVPTEVAAA
                     ALVIVGALMMSQIRFIDLTDFSALFGVFLTVAVMPMTYSIANGIGAGFIGWVAARAFS
                     GKAKEISPLLWIVTAGFVIFFARGPIEALLA"
     gene            complement(1878..2342)
                     /locus_tag="VN041_05100"
     CDS             complement(1878..2342)
                     /locus_tag="VN041_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU28456.1"
                     /translation="MEITSFYPVLMVEDVGAAARFYRETLGCETTFEADWYVSLRFDG
                     GELAVLDSTHETIPEGFRTPVTGLLLNIEVEDAKAEHDRLVRARAHGATDAARRGLRP
                     AALHRRGSGRRAARRDRAHRADGGVRRRVRLSDRERVRTTEGPDARHPAPPS"
     gene            2418..>2692
                     /locus_tag="VN041_05105"
     CDS             2418..>2692
                     /locus_tag="VN041_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28457.1"
                     /translation="MPRASAAEAAATAARVLDAAEKRFTAQGFGAASVDDIAADAGVT
                     RGAVYHHYDSKPGLFLAVVQRMQQQVADAVVAAAGDATPADSLRLGS"
BASE COUNT          405 a          958 c          884 g          445 t
ORIGIN      
        1 ccgcgatcgc cgcacggcgg ccggagcggg ccgcgaagat cacggtgaac agcaggatcg
       61 ccagcggcgg cacggacagc gcgaaggtcg ccgcctcctt ggggatcgcg agcccggaca
      121 gcacggcatc cggcagcacg acctgcagcg gcacgccgtg cccgaactgc cagagcgtgc
      181 cgctgaccgg ccacagggcg ccccagtcgg cgtccactcc gaacgacagc acccatagca
      241 gcgtgaacgg cgcgagcacg gcggccaggc ctatggcggc cgcgatggcg gcgtccaggg
      301 cggcgaggag caggacgatg acgcgttgca tgactccacg agcctacggc ccgcatccga
      361 tgggacagcc gagcccacgc gagaggggcg caacacgata gattctcctg cggaggtcac
      421 gatgacaact gcccctgcgc accccgattc cgacaccgag ccgcgcaacg cgctcgaccg
      481 attcttcgag atcacgaagc gggggtcgag cttcggcgcc gagatccgcg gtggcgtggt
      541 caccttcgtg acgatggcgt acatcgtcat cctgaacccg atcatcctgt ccacgaccgc
      601 tgacatcgat gaccacaagc tgggcttcct gcagctgagc gcggtcaccg gcctcaccgc
      661 cgcggtgatg acgatcctgt tcggtctcat cgcccgcctg ccgttcggct tcgccgcagg
      721 cctgggcatc aacgccttcc tcgccttctc ggtggtcggc gaggtcacct ggcccgaggc
      781 gatgggcctc gtggtcatca acggcctcat catcgtgctg ctcagcgcca ccggcctgcg
      841 ccgcatgatc ttcgacgcgg tcccgatgca gctgaagatc gcgatcacgg tcggcatcgg
      901 cctgttcatc gcgttcatcg gcttcgtcaa cgccggcttc gtcaccgccg cgggctcccc
      961 gccggtcacg ctcggcatcg gcggatccgt cagcacggtt ccgaccgtga tcttcgtctt
     1021 cacgctgctg ctgaccggca tcctcgtcgc ccgcaaggtg aagggtggca tcctcatcgg
     1081 cctggtctcc ggcaccgtgc tcgccgtgat cgtcgaggcg atctggcaca tcggcgccaa
     1141 gggcgagaag aaccccggcg gctggggcct gtcggtgccc gccctcccca aggagctcgt
     1201 cagcctcccc gacctctcgc tggtcggcca ggtggacctg ttcggcgcgt tcggccggat
     1261 cggggcgctc gccgccctga tgctcgtgtt cacgctcgtg ttcaccaact tcttcgatgc
     1321 catgggaacc atgaccggtc ttgccaagga gtccgggctc gcagacgaga agggcaactt
     1381 cccgcgcctg aagtcggcgc tgatcgtcga gggcgtcggc gccgtggtgg gcggcgcgac
     1441 ctcgggctcg tcgaacaccg tgttcatcga gtcgggtgcc ggtatcggcg agggagcccg
     1501 caccgggctc gcgaacatcg tcacgggtgc gctgttcctg atcgcgatgt tcttcacgcc
     1561 gctggtctcg atcgtgccga ccgaggtggc ggcggcggcg ctggtgatcg tcggtgcgct
     1621 gatgatgagc cagatccgtt tcatcgacct gaccgacttc tcggcgctgt tcggcgtctt
     1681 cctgacggtc gccgtcatgc cgatgaccta ctcgatcgcc aacggcatcg gcgcgggctt
     1741 catcggctgg gtggccgccc gggcgttctc cggcaaggcc aaggagatca gcccgctgct
     1801 gtggatcgtc acggccggct tcgtgatctt cttcgcccgc gggccgatcg aggcgctgct
     1861 cgcctgatcg cgtctgatca cgacggaggg gccggatgcc gagcatccgg cccctccgtc
     1921 gtcctcacgc gctcacggtc gctcaggcgt acgcgtcgac gaactccgcc gtcggcgcga
     1981 tgggctcgat cacgtcgagc agcacgccgc ccggagcctc gacgatgaag tgccgctggc
     2041 cgaaggcctc gtcgcgcagc gtcagtcgct ccgtgagccc gagcgcgcac gagtcggtcg
     2101 tgctccgcct tggcatcctc gacctcgatg ttcagcagca gcccggtcac cggcgtgcgg
     2161 aagccctccg ggatcgtctc gtgcgtgctg tccaggacgg cgagctctcc cccgtcgaag
     2221 cgcaggctga cgtaccagtc cgcctcgaag gtcgtctcgc agccgagcgt ctcgcggtag
     2281 aaccgcgccg cggcgccgac gtcctcgacc atgagcacgg ggtagaacga ggtgatctcc
     2341 atgggtcatc ccttacatac tctgtgtttg tatcaggtga ggctagaata catgcacagc
     2401 gcacgtaaag gaatcccatg cccagagcct cagccgcaga agccgcggcg accgccgcgc
     2461 gcgtgctcga cgccgcagag aagcgcttca ccgcgcaggg gttcggcgcc gcatccgtcg
     2521 acgacatcgc cgccgacgcc ggcgtgaccc ggggcgccgt gtatcaccac tacgactcca
     2581 aacccggcct gttcctcgcc gtcgtgcagc gcatgcagca gcaggtggcc gatgccgtgg
     2641 tcgccgctgc cggcgatgcg accccggccg actcgctgcg cctcggcagc ca
//
LOCUS       DATMSX010000167         6857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_182631,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000167 DATMSX010000000
VERSION     DATMSX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182631"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VN041_05110"
     CDS             complement(<1..543)
                     /locus_tag="VN041_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWU28458.1"
                     /translation="MTSHTIAPVVPARGRLRPLGIDEVRIDDGFWAERQRVNGANTLA
                     HIAAKLESEGWLPNFDLAAAGTLPQGRRGREFADSEVYKYLEALAWEIGRTGDEDLEQ
                     RFRAIVARVAAAQEDDGYLNTRFGRPGQGERWSDLEWGHELYCLGHLFQAAVARGRTR
                     PDADDGLLDVAVRAADLVCRE"
     gene            complement(545..1432)
                     /locus_tag="VN041_05115"
     CDS             complement(545..1432)
                     /locus_tag="VN041_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28459.1"
                     /translation="MTTTVPDTTRTIVAPGAVHRQSRRMPLAFGIPYWVFTGALALIF
                     LYPLIWTAVSSVSPLAGTSQTEGWGFGNYAALTEYQAGIWVYLWNSTVVSVLTVALTL
                     VISLLGGYAFARFSFPGKNALFLLTLAILMVPYATLLIPLYVILNAVGLQNSLVGVAL
                     VITMFQLPFSMFMMRISFESIPRELDEAAMVDGCSSWSALWRVLLHAVKPGLVTVGLF
                     AFLTAWNDFMAPLILINDSNRMTLPLAVANLRGQVQGVVDYGATEAGVVVLALPCILL
                     FLILQRHYVRGFMSGAFKG"
     gene            complement(1437..2366)
                     /locus_tag="VN041_05120"
     CDS             complement(1437..2366)
                     /locus_tag="VN041_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28460.1"
                     /translation="MALTASAPPATAPGTTPRRRRRRGYGGPVQGWLYASPTAIFVAC
                     LFIVPLVLVFQMSASDWPLLGGNQGVNLPENYVDAVNHRLFWDSIRFTLLYTVITTVI
                     LITLGLGLALLVQESTRWKGFLRTAFLVPSALGLASASLLFYVLYSPIAGPFADLMKS
                     WGITFLGTPTGALWSTIFLIVWRYAGFYMLLMLVGLQGIPDDVYEAARIDGASRWQTF
                     RDITVPLLRPTFALTTIMCVTGSLLAFEQFYILTKGGPDNSTMTIVQLIYNVAFQGQN
                     SLGIAGALSVIVLLALVVVNVFQMRAFGRSDES"
     gene            complement(2441..3736)
                     /locus_tag="VN041_05125"
     CDS             complement(2441..3736)
                     /locus_tag="VN041_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU28461.1"
                     /translation="MHTTGKRRLLRGLAALAAAGTIAGGLAACAPASDDKPAGPITAD
                     GTDDGAKLTLWTRAPMERQAKLLVEAYNDSHKNQVELTVVPNDDYVAKVGAAAGSGGL
                     PDLFAADIVYVPNWVKQGLFTDITKQIDGLDYKDEINQGHLNAGTLDGKEHVLPFVLD
                     LSMLMWNKELFKEAGLDPEKAPATLAEFADAAEKVQQLNKPDTYGTATGLNCGGCLVF
                     TWFPSIWASGDQVMNEDGTKALLDSDSAKAVYDTWKGLADAGAVLPSSTDEAGPTWTA
                     AFSEGKVGVMPFPATLLSSLPFEAGVAGIPGVDGGASTFVGGDGIGISKDSKKAAQAW
                     NFLSWMMSEDAQVGVLAKDDNAVSRGDLANNEYAAKDPRLVTINEVAAKGDTPVALNF
                     QQAFNAPGSPWLTLVRNAVLKGTDTVDADDKEITAVLGQ"
     gene            3900..4919
                     /locus_tag="VN041_05130"
     CDS             3900..4919
                     /locus_tag="VN041_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28462.1"
                     /translation="MANGSTRAVTLSDVASAAGVSISTASKALNDREDVSAATKARVL
                     AVAERMSFTPNVMARGLLAGRTGTVGLLTSDLEGRFMIPILMGAEDAFGAGRINVFLC
                     DARGDAIREQHHLRELLSRRVDGIIVVGRQTDPRPSLGQSIPVPVVYAYAPSDDPRDV
                     SLTPDNVAGGRLAIEHLLACGRTRIAHISGDPAYAAAQDRLEGARAALADAGLELIGA
                     PLLSEWTEQWGRDAGAMLLQQHPDIDAIFCGSDQIARGVLDSARDLGRSVPDDLAVIG
                     YDNWSILVENSRPELTSIDANLQLLGRRAAEKVFEAIDGAETQGGTLQLPVRLVIRGS
                     TIAHR"
     gene            5034..6671
                     /locus_tag="VN041_05135"
     CDS             5034..6671
                     /locus_tag="VN041_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HWU28463.1"
                     /translation="MPAATTQSTTTHSTKREAFGSRNVFILSAIGSAVGLGNIWRFPY
                     VAYEGGGGAFLIPYLCALLTAGIPLLFFDYAIGHRFRGSSPLAFRRMHRAAEPLGWWQ
                     VLICVVIATYYAVIIAWAAMYTWFSAQVTWGAGNENDFFYKDFLQMGDVSKSGVSTQF
                     VPQVGLPLIAVWLVVIVIMGLGVKRGIGRANTVLMPLLTVMFAALVVQSLFLPGAVDG
                     LNAFFTPNWKALADPAVWASAYGHIFFSLSVAFGIMVTYSSYLKRKTDLTGSGLVVAF
                     ANSGFEILAGIGVFAALGFMAQAQGTDVAHTATAGIGLAFVAFPTIVSNAAGGSIIGV
                     LFFGALVFAGVTSLISVLEVVVAALQDKLGWGRVRTTLVVSIPIALISIAMFSTTTAL
                     AVLDTTDAFVNAFGIMAVALVAVIVVAWLLHRLPALRDHLNRRSSFTVGWFWMLLVGA
                     LAPIVLGYLFVSEVIAKVSEPYGGYPGWFLSIFGWGMVIALVVLAILLSLLPWSHRSH
                     AKDDPDYDEFLVEESYAPDAETGAVRLPDAVAEGARS"
     gene            6668..6802
                     /locus_tag="VN041_05140"
     CDS             6668..6802
                     /locus_tag="VN041_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HWU28464.1"
                     /translation="MTPIAVIMMIIAMVTVWGGLIAGIVNLARHPEVADAEPQPPAEL
                     "
BASE COUNT         1054 a         2283 c         2371 g         1149 t
ORIGIN      
        1 ctcgcggcag accaggtcgg cggcgcggac ggcgacgtcg agcaggccgt cgtcggcatc
       61 cggccgtgtg cgcccgcggg cgactgctgc ctggaacagg tgtcccaggc agtacagctc
      121 gtggccccat tccaggtcgg accatcgctc gccctggccc ggcctgccga acctcgtgtt
      181 caggtagccg tcgtcctcct gcgcggcggc gacccgtgcg acgatcgcgc ggaagcgctg
      241 ctccagatcc tcgtcgccgg tgcggccgat ctcccaggcc agcgcctcga ggtacttgta
      301 cacctcggag tcggcgaact cgcgaccgcg acgcccctgc ggcagggtgc cggcggccgc
      361 cagatcgaag ttgggcagcc agccctcgga ctccagcttc gccgcgatgt gggcgagcgt
      421 gttcgcgccg ttcacgcgct ggcgctcggc ccagaaaccg tcgtcgatgc ggacctcgtc
      481 gatgccgagc ggtcgcaggc gaccgcgggc ggggacgacg ggagcgatgg tgtgagaagt
      541 catgtcagcc cttgaaagcg cccgacatga agccgcgcac gtagtgtcgt tgcaggatca
      601 ggaacagcag gatgcacggc agtgcgagca ccaccacgcc ggcctcggtg gcgccgtagt
      661 cgaccacgcc ctgcacctgg ccgcgcaggt tggcgaccgc caggggaagg gtcatccggt
      721 tgctgtcgtt gatgaggatc agcggcgcca tgaagtcgtt ccaggcggtg aggaaggcga
      781 acaggccgac cgtcaccagt ccgggcttga cggcgtgcag cagcacgcgc cacagcgcgc
      841 tccagctgga gcatccgtcg accatcgccg cctcgtcgag ctcccgtggg atcgactcga
      901 acgagatgcg catcatgaac atcgagaagg gcagctggaa catcgtgatc accagcgcca
      961 ccccgaccag ggagttctgc aggccgaccg cgttcaggat cacgtacagc gggatcagca
     1021 gggtggcgta cggcaccatc aggatggcca gggtcagcag gaacagcgca ttcttgccgg
     1081 ggaacgagaa ccgtgcgaag gcgtagccgc ccagcagcga gatgaccagg gtcagcgcca
     1141 cagtcagcac cgagacgacg gtcgagttcc acaggtacac ccagatgccc gcctggtact
     1201 cggtcagggc tgcgtagttg ccgaagcccc agccctcggt ctggctggtg ccggccagcg
     1261 ggctcacgct cgagacggcg gtccagatca acgggtagag gaagatcagc gcgagcgcgc
     1321 cggtgaacac ccagtacggg atgccgaacg cgagcggcat ccgcctggac tgccggtgaa
     1381 cagcgccggg ggcgacgatc gtgcgggtcg tgtcggggac ggtcgtggtc atggtctcag
     1441 ctctcgtccg agcgcccgaa ggcgcgcatc tggaagacgt tcacgacgac gagggcgagc
     1501 agcacgatca ccgagagggc gccggcgatg ccgaggctgt tctgaccctg gaaggcgacg
     1561 ttgtagatga gctgcacgat ggtcatcgtg gagttgtcgg ggccgccctt ggtgaggatg
     1621 tagaactgct cgaaggcgag cagggatcct gtcacgcaca tgatcgtggt gagtgcgaag
     1681 gtgggccgca gcagcggcac ggtgatgtcg cggaaggtct gccagcggct cgcgccgtcg
     1741 atgcgggcgg cttcgtacac atcgtccggg atgccctgca ggcccaccag catgagcagc
     1801 atgtagaagc cggcgtagcg ccagacgatc aggaagatgg tcgaccagag agcgccggtg
     1861 ggcgtgccga ggaacgtgat gccccacgac ttcatcagat cggcgaaggg tcccgcgatc
     1921 ggcgagtaga gcacgtagaa cagcagggat gcggatgcca ggcccagggc gctcggcacc
     1981 aggaacgccg tgcgcaggaa tcccttccag cgggtggact cctgcaccag cagcgcaagc
     2041 cccagcccga gggtgatcag gatgacggtg gtgatgaccg tgtacagcag cgtgaagcgg
     2101 atcgagtccc agaacagccg gtggttcacg gcgtcgacgt agttctccgg gaggttgacg
     2161 ccctggttgc cgcccagcag aggccagtcg gacgccgaca tctggaagac cagcaccaga
     2221 gggacgatga acaggcaggc gacgaagatc gccgtggggg aggcgtacag ccacccctgg
     2281 acggggccgc catagccgcg gcggcgcctg cgacgcggcg tcgtgcccgg tgccgtcgcc
     2341 ggcggggcgg aagcggtgag tgccatctcg atccaatcgg tttcggcgga agcgttgcgg
     2401 gggctcgggc ggacggccgt ccggccgtcc gcccgacggg tcactggccg aggacggccg
     2461 tgatctcctt gtcgtccgcg tcgacggtgt cggtgccctt gagcaccgcg ttgcgcacca
     2521 gggtcagcca cgggctgcca ggggcgttga acgcctgctg gaagttcagc gccacggggg
     2581 tgtcgccctt ggcggccact tcgttgatgg tgaccaggcg cgggtccttc gccgcgtact
     2641 cgttgttcgc gaggtcgccg cgcgagacgg cgttgtcgtc cttggcgagc acgccgacct
     2701 gggcgtcctc ggacatcatc cagctgagga agttccacgc ctgcgcggcc ttcttcgagt
     2761 ccttcgagat gccgatgccg tcgccgccga cgaacgtcga tgcgccgccg tcgacacccg
     2821 ggatgccggc cacgcccgcc tcgaacggga gcgaggagag cagtgtggcg gggaacggca
     2881 tgacgccgac cttgccctcg ctgaacgccg cggtccacgt cggccccgcc tcgtcggttg
     2941 agctgggcag caccgcgccg gcgtctgcca gacccttcca ggtgtcgtag acggccttgg
     3001 ccgagtcgct gtccagcagc gccttggtgc cgtcctcgtt catcacctgg tcgcccgacg
     3061 cccagatgga ggggaaccag gtgaacacca ggcatccgcc gcagttcagg ccggtggcgg
     3121 ttccgtaggt gtcgggcttg ttcagctgct gcaccttctc ggcagcgtcc gcgaattccg
     3181 cgagcgtcgc cggagccttc tcggggtcga ggccggcctc cttgaagagc tccttgttcc
     3241 acatcagcat cgacaggtcg agcacgaacg gcagcacgtg ctccttgccg tccagggtcc
     3301 cggcgttcag gtgcccctgg ttgatctcat ccttgtagtc cagcccgtcg atctgcttgg
     3361 tgatgtcggt gaacaacccc tgcttgaccc agttgggcac gtagacgatg tcggcggcga
     3421 acaggtcggg cagaccgccc gaaccggcgg cggcgccgac cttggcgacg tagtcgtcgt
     3481 tgggcacgac ggtgagctcc acctggttct tgtgggagtc gttgtaggcc tccacgagaa
     3541 gcttggcctg ccgctccatg ggtgcgcggg tccacagtgt cagcttggcg ccgtcgtcgg
     3601 tgccgtcggc ggtgatcggc ccggcgggct tgtcgtcgga tgccggggcg caggctgcaa
     3661 gaccgcccgc gatcgtcccc gccgcggcca gcgcggcgag accgcgcaac aggcggcgct
     3721 ttccggtggt gtgcatgtga tgtctcctct ggctcttctt cgagcggatg ctgggcttgc
     3781 accgccgagg gcaattatcg aaatctacga aaaccctttc gatcatgtat agcgcggaca
     3841 gatgcggccg gtcaaggatg aatccgataa cctttcgcta ccggcagtga ggggacgcga
     3901 tggccaacgg ctcaaccagg gcagtgacgc tgagcgacgt cgccagcgcc gcaggagtgt
     3961 ccatctcgac ggcgtcgaaa gcgctgaacg accgtgagga cgtctccgcg gccacgaagg
     4021 cgcgtgtgct cgcggtcgcc gagcggatgt cgttcactcc gaacgtgatg gcccgcgggc
     4081 tgctcgccgg gcgcaccggt acggtgggcc tgctcaccag cgatctcgag ggccggttca
     4141 tgatccccat cctgatgggg gcggaggatg ccttcggagc ggggcgcatc aatgtcttcc
     4201 tgtgcgacgc gcgcggcgat gcgatccgcg agcagcacca cctgcgcgag ctgctcagcc
     4261 ggcgcgtcga cgggatcatc gtcgtcggcc gccagacgga tccgaggccg tcgctcggtc
     4321 agagcatccc cgtccccgtc gtctacgctt acgctccctc ggatgacccg cgcgatgtct
     4381 cgctcacgcc cgacaacgtc gccggtggac gattggccat cgagcacctg ctcgcgtgcg
     4441 gacgcacccg catagcgcac atctcgggcg atcccgccta tgctgccgcg caggaccgcc
     4501 tcgagggcgc ccgggctgca ctggcggacg ccggcctgga gctgatcggt gcccctctgc
     4561 tctccgagtg gacagagcag tgggggcgcg atgccggtgc gatgctgctc cagcagcatc
     4621 cggacatcga cgcgatcttc tgcggatcgg accagatcgc ccgcggagtg ctcgattcgg
     4681 cgcgcgatct cggccgatcc gtacccgacg atctcgccgt gatcggctac gacaactgga
     4741 gcatcctggt ggagaactcc cggccggagc tcacctcgat cgatgccaat ctgcagcttc
     4801 tcggccgtcg cgcggccgag aaggtgttcg aggcgatcga cggggcggag acgcaaggcg
     4861 gcacgctcca gctccccgtc cggctggtga tccgcggttc gaccatcgcg catcgatgat
     4921 ccagccccga cagggcagcg gagacagggg acggaatgct gctcccgctc gccagcctca
     4981 agcttccgtc aactccctga ccccgccttc gcccctcagg tagcctcgag gacatgccag
     5041 ccgcgacgac gcagtccacg acgacgcact ccaccaagcg cgaggccttc ggctctcgca
     5101 acgtgttcat cctctcggcc atcggatcgg cggtcggcct gggcaacatc tggcgcttcc
     5161 cgtacgtcgc ctacgagggc ggcggcggcg ccttcctcat cccgtatctg tgcgcactgc
     5221 tgaccgccgg catcccgctg ctgttcttcg attacgcgat cgggcaccga ttccgggggt
     5281 cctctccgct ggcgttccgc cgcatgcacc gcgcggccga gccgctgggg tggtggcagg
     5341 tgctgatctg cgtcgtgatc gcgacgtact acgcggtgat catcgcgtgg gcggcgatgt
     5401 acacctggtt ctccgcgcag gtcacctggg gggccggcaa tgagaacgac ttcttctaca
     5461 aggacttcct gcagatgggt gacgtctcca agagcggcgt gtccacgcag ttcgtgccgc
     5521 aggtgggcct cccgctgatc gccgtgtggc tcgtggtcat cgtgatcatg ggcctcggcg
     5581 tcaagcgagg catcggccgt gcgaacaccg tgctcatgcc gctgctgacc gtcatgttcg
     5641 cggcgctcgt ggtgcagtcg ctcttcctgc cgggcgcggt cgacggcctg aacgcgttct
     5701 tcaccccgaa ctggaaggcc ctcgcggacc cggcggtgtg ggcctcggcc tacgggcaca
     5761 tcttcttctc gctgtcggtc gcgttcggca tcatggtcac ctactcttcg tacctgaagc
     5821 gcaagaccga cctgacgggt tccggcctgg tggtcgcctt cgcgaactcc ggcttcgaga
     5881 tcctcgccgg catcggcgtg ttcgccgcgc tgggcttcat ggcgcaggcg cagggcacgg
     5941 atgtcgcgca caccgcgacg gcgggcatcg gcctggcgtt cgtcgccttc cccacgatcg
     6001 tctcgaacgc ggcgggcgga tcgatcatcg gagtgctgtt cttcggcgcg ctggtcttcg
     6061 ccggtgtcac ctcgctgatc tcggtgctgg aggtggtggt cgccgctctc caggacaagc
     6121 tcggctgggg tcgcgtccgc accacgctgg tcgtctccat cccgatcgca ctgatctcga
     6181 tcgcgatgtt ctcgacgacc accgcgctcg ccgtgctcga caccaccgac gcgttcgtca
     6241 acgccttcgg catcatggcc gtcgcgctgg tcgcggtgat cgtcgtcgcc tggctgctgc
     6301 atcggctgcc ggcgctgcga gaccacctca accgtcgttc gagcttcacg gtcggctggt
     6361 tctggatgct gctggtcggt gcgctcgcgc cgatcgtgct cggctacctg ttcgtgagcg
     6421 aggtcatcgc caaggtctcc gagccgtacg gcggctaccc gggctggttc ctctcgatct
     6481 tcggctgggg catggtgatc gccctcgtcg tgctggcgat cctgctgtcg ctgctgccgt
     6541 ggagtcaccg ctcgcacgcc aaggacgatc cggactacga cgagttcctc gtggaggaga
     6601 gctacgcgcc ggatgcggag accggcgcgg tcagactgcc ggacgccgtc gcggaaggag
     6661 cccggtcatg accccgatcg ccgtcatcat gatgatcatc gcgatggtca cggtctgggg
     6721 cggcctgatc gccgggatcg tcaacctcgc ccgccacccc gaggtcgccg acgccgagcc
     6781 gcagccgccc gccgaactgt agggcgcggc gggggtcaca cccgcggagc gcccgtcgac
     6841 tcgcgccagc ggatcgt
//
LOCUS       DATMSX010000168         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_49677,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000168 DATMSX010000000
VERSION     DATMSX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49677"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="rho"
                     /locus_tag="VN041_05145"
     CDS             <1..1434
                     /gene="rho"
                     /locus_tag="VN041_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWU28465.1"
                     /translation="QSADARSPAPPVRRRSRGRGGKGENGQNGQNGQNGGQQGGQNSR
                     GGQQDDAQGDDEQGSGRTRQRNKRRGGANQNADEFETEIGEDDVLIPVAGILDVLDNY
                     AFVRTTGYLAGASDVYVSLGQVKKYNLRKGDAIVGAIKQPREGEQQGRQKYNALVKVD
                     SINGLSPEDAGNRVEFGKLTPLYPQERLRLETAPEKLTQRIIDLVAPIGKGQRGLIVA
                     PPKAGKTIVLQQIANAIAQNNPEVHLMVVLVDERPEEVTDMQRTVKGEVIASTFDRPA
                     EDHTTVAELAIERAKRLVELGRDVVVLLDSITRLGRAYNISAPASGRVLTGGVDASAL
                     YPPKRFFGAARNIENGGSLTILATALVETGSKMDEVIFEEFKGTGNSELRLSRALADK
                     RIFPAVDVNASSTRREEMLLSADEVKITWKLRRALAGLDPQQALEVVLGKLKETSSNV
                     EFLFQMQKSIPAPTSTGSSHAHENNIR"
     gene            1455..2537
                     /gene="prfA"
                     /locus_tag="VN041_05150"
     CDS             1455..2537
                     /gene="prfA"
                     /locus_tag="VN041_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWU28466.1"
                     /translation="MFESVQALIDEHRRVQEELSDPAVHADAARAKRVNRRYAELSKI
                     VAAHEAWVAAGEDLEAARELAREDEAFADEVPELEAGLHAAQEKLRRLLIPRDPDDAR
                     DVIMEIKAGEGGAESALFAADLLRMYIQYAAHRGWKTELLERNESDLGGYKDVQVAIK
                     GSSSDPSQGVWAHLKYEGGVHRVQRVPATESQGRIHTSTTGVLVFPEVDEPEEIQIDP
                     NDLKIDVYRSSGPGGQSVNTTDSAVRITHVPTGIVVSMQNEKSQLQNREAGMRVLRAR
                     LLARQQEELAAAASDARRSQIRGMDRSERIRTYNFPENRIADHRTGYKAYNLDQVMDG
                     ALEPIIESCIEADEAERLAAVGSDAS"
BASE COUNT          462 a          898 c          895 g          337 t
ORIGIN      
        1 cagtctgccg acgctcgatc gcctgcgccg cctgtccgcc gccgaagccg cggtcgtggt
       61 ggcaagggcg agaacggcca gaacggtcag aacggccaga acggcggcca gcagggcggc
      121 cagaacagcc gcggtggcca gcaggacgac gcccagggcg atgacgagca gggctccggc
      181 cgcacccgcc agcgcaacaa gcgccgtggc ggcgcgaacc agaacgccga cgagttcgag
      241 accgagatcg gcgaggacga cgtgctgatc cccgtcgcgg gcatcctcga cgtgctcgac
      301 aactatgcgt tcgtgcgcac caccggctac ctcgccggcg ccagcgacgt ctatgtctcg
      361 ctcggccagg tcaagaagta caacctgcgc aagggcgacg cgatcgtcgg cgccatcaag
      421 cagccgcgcg agggcgagca gcagggccgc cagaagtaca acgccctggt caaggtcgac
      481 tcgatcaacg gcctctcgcc cgaggacgcg ggcaaccgcg tcgagttcgg caagctgacc
      541 ccgctgtatc cgcaggagcg cctgcgtctg gagacggcac ccgagaagct gacccagcgc
      601 atcatcgacc tggtcgcccc gatcggcaag ggccagcgcg ggctgatcgt cgcgccgccc
      661 aaggccggca agaccatcgt gctgcagcag atcgcgaacg cgatcgcgca gaacaacccc
      721 gaggtgcacc tcatggtcgt gctcgtcgac gagcgccccg aagaggtcac cgacatgcag
      781 cgcacggtga agggcgaggt catcgcgtcc accttcgacc gtcccgccga ggaccacacc
      841 acggtcgccg agcttgccat cgagcgcgcg aagcgactgg tggagctggg ccgcgacgtg
      901 gtcgtgctgc tcgactcgat cacccgactg ggccgcgcgt acaacatctc ggcgcccgcc
      961 tccggccgcg tgctgaccgg tggtgtcgac gcttcggcgc tgtacccgcc gaagcgcttc
     1021 ttcggcgccg cgcgcaacat cgagaacggc ggctcgctga ccatcctcgc gaccgcgctc
     1081 gtggagaccg gctccaagat ggacgaggtc atcttcgagg agttcaaggg caccggcaac
     1141 agcgaactgc gcctgtcgcg cgcgctggcc gacaagcgga tcttcccggc ggtggacgtc
     1201 aacgcgtcca gcacgcgccg cgaggagatg ctgctctcgg ccgacgaggt caagatcacc
     1261 tggaagctgc gccgtgccct cgcgggcctg gaccctcagc aggccctgga ggtcgtgctc
     1321 ggcaagctga aggagacgag ctcgaacgtc gagttcctgt tccagatgca gaagtcgatc
     1381 ccggcgccca cctcgaccgg ttcctcgcac gcgcacgaga acaacatccg ctgaactgca
     1441 gtcgggactg atctgtgttc gagtccgtcc aggctctgat cgacgagcat cgccgggtgc
     1501 aggaggagct ctccgacccg gcggtgcacg ccgatgccgc gcgggcgaag cgcgtcaacc
     1561 gtcgctacgc cgagctgtcg aagatcgtcg ccgcacacga ggcatgggtc gccgcaggcg
     1621 aggatctgga ggccgcccgt gagctcgccc gcgaggacga ggcgttcgcc gacgaggttc
     1681 ccgagctgga ggccggcctg cacgccgcgc aggagaagct gcgccggctg ctgatcccgc
     1741 gcgacccgga cgacgcccgc gacgtgatca tggagatcaa ggcgggggag ggcggcgccg
     1801 agtcggcgct gttcgccgcg gacctgctgc ggatgtacat ccagtacgcc gcgcaccgcg
     1861 gctggaagac cgagctgctc gagcgcaacg agtccgatct cggcggctac aaggacgtgc
     1921 aggtggcgat caaggggtcc tccagcgatc cctcgcaggg cgtctgggcg cacctgaagt
     1981 atgagggcgg cgtgcaccgc gtgcagcggg tgccggcgac cgagtcgcag ggacgcatcc
     2041 acacctcgac caccggtgtg ctggtcttcc ccgaggtcga cgagccggag gagatccaga
     2101 tcgatccgaa cgatctgaag atcgacgtgt accggtcgtc cggtcccggc gggcagtccg
     2161 tgaacaccac cgactcggcc gtgcgcatca cgcatgtgcc gaccggcatc gtggtgtcga
     2221 tgcagaacga gaagtcgcag ctgcagaacc gcgaagccgg catgcgcgtg ctgcgcgccc
     2281 ggctgctggc gcgtcagcag gaggagctgg cggccgcggc atccgacgcc cgtcgctcgc
     2341 agatccgcgg tatggaccgc tcggagcgca tccgcaccta caacttcccg gagaaccgca
     2401 tcgcggacca tcgcacgggg tacaaggcgt acaacctcga ccaggtgatg gacggcgcgc
     2461 tcgagccgat catcgagtcc tgcatcgagg ccgacgaggc cgagcgcctc gccgccgtcg
     2521 gctccgacgc ctcctgaccc gccgtcggcg cgcggccgct cagatcgagt agtcggggac
     2581 ggacagcagg gc
//
LOCUS       DATMSX010000169         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_116175,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000169 DATMSX010000000
VERSION     DATMSX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116175"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VN041_05155"
     CDS             <1..192
                     /locus_tag="VN041_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWU28467.1"
                     /translation="AVGELSLAGEIRPVTQAPQRRSEAARLGYRQVVDDRSKTLRSAL
                     SDIRSHTTERRPEADIPPF"
     gene            complement(227..469)
                     /locus_tag="VN041_05160"
     CDS             complement(227..469)
                     /locus_tag="VN041_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28468.1"
                     /translation="MTSYRAVFDAEITFSNDGGLQAQGFRVDIPGPEITDEEIGRLFV
                     TSLDLLMADTVVISNVRIIEEAHKGTRGGPSDPRAS"
     gene            complement(469..879)
                     /locus_tag="VN041_05165"
     CDS             complement(469..879)
                     /locus_tag="VN041_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HWU28469.1"
                     /translation="MAKKRAGAKRPAPQDFRSEALAQALEKQDMAAVALALRNGNTVV
                     PLIKPGPRDNPLDGGEVWTYRDAGTGEVALLLFSDAKHKPANLPPGVGIYSPAWLRAF
                     LTTHRETITTVFLDIAGPHPMQAAPDELLKALEA"
     gene            complement(1142..1804)
                     /locus_tag="VN041_05170"
     CDS             complement(1142..1804)
                     /locus_tag="VN041_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28470.1"
                     /translation="MPRQSAAVYRRRRLAVLLILIVVLAVVTGGLWVAIAQPWKTGAE
                     PAPKTPSAQPTDGTATDAPPATPSDSASPDPSGDPSAPPGVVACEAGDVEVTAVTDAE
                     TYAADALPKLSITLTNKSDRDCTMNVGSATQKFTITSGNDVWWRSTDCQTDPSDQIAT
                     LTAGATVSTKTPVVWDRTRSAVGTCDQQNRARAPGGGASYHVEVSIGGFASASTRQII
                     LR"
     gene            complement(1814..2317)
                     /locus_tag="VN041_05175"
     CDS             complement(1814..2317)
                     /locus_tag="VN041_05175"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HWU28471.1"
                     /translation="MSEYTLRAARTADINGIHALLEPLVERRILLGKDLAILYGAVQE
                     FVVAEVDGELIGCGALHVMWEDLGEVRTLLVRDDFLHHGVGRAIVEALEQRARELGLS
                     RLFCLTFETGFFTRRGFEPIGEHIVDPDVYSQLLRSGDAGVEEFLDLAHVKPNTLGNT
                     RMLKHLV"
     gene            complement(2405..2794)
                     /locus_tag="VN041_05180"
     CDS             complement(2405..2794)
                     /locus_tag="VN041_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWU28472.1"
                     /translation="MSAQIVRRDVVVASLTDLKERERIVFDVDGTEFGVYFHDGEVRA
                     WYNVCPHQGGPVCQGKIMPRTVQLVRGEGGLKSGGPGHHPSDRNIVCPWHGFEFDILT
                     GKHPADQSVGLRRLPVRVEGDDVIVTV"
     gene            complement(2791..3102)
                     /locus_tag="VN041_05185"
     CDS             complement(2791..3102)
                     /locus_tag="VN041_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28473.1"
                     /translation="MTETNTTTAELAELEGLVARLGELTHHVTEQELGAEVPDEQIAD
                     ILYAAARLFSAKRDRVGKIAWPIREDALNATETVVLVTALLDAADVNLFDMAIWYRRA
                     E"
     gene            complement(3102..4367)
                     /locus_tag="VN041_05190"
     CDS             complement(3102..4367)
                     /locus_tag="VN041_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU28474.1"
                     /translation="MSDLPTPRSDAPRIRELAEIGGRSDTRDVLAHATKQAQESYDDY
                     FIVDIDAHVSEDHFWKEVLDLIDNDVWKQIGLQQYAGFGGGNALLNIQPGMSHQSVGG
                     RIGHQGRKEPVGPDDGHPFVTAARRGMDAMGLDYQVVFPSAMLLLGMHPQDEVEVVLG
                     RAFNRWLTEVILPPNPRLKGMLYLPYNTPEVCEELVEKYADDPGIIGYTVCSTRNNPV
                     HSDVYTRLYKLIEDSGKPLAFHSGYHWGDPSFSQLNRFLSMHALSFTHYNQIHVTNWI
                     INAMPERFPKLKMVWVESGLAWIPFLMQRLDHEVLMRPSEAPGLKRLPSEYMKEMWYT
                     TQPMERTNMELLEATMKSFNAETQLLYSSDWPHWDWDAPSTITRLPFLSEQAKRNILG
                     LNAARVFNLPTDRQKPSAEQVLQDRPGIS"
     gene            complement(4391..>4678)
                     /locus_tag="VN041_05195"
     CDS             complement(4391..>4678)
                     /locus_tag="VN041_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28475.1"
                     /translation="RPPARGQFCLGINCVPDADRYADHGSAVNLLVRADGIPLRVRVE
                     ISELSADEIPREGEQLSFAVAPARAVVISQQKQQSESEYLTVATNAIVSPR"
BASE COUNT          704 a         1557 c         1626 g          791 t
ORIGIN      
        1 gccgtcggcg agctgtcgct ggccggagag atccgtcccg tgacgcaggc cccgcagcgc
       61 cgcagcgaag ccgcccggct cggctaccgc caggtggtcg acgaccgctc gaagacgctg
      121 cgctccgcgc tcagcgacat ccgctcgcac acgaccgagc gacgccccga agcggacatc
      181 ccgccgttct gatcaggcat cgagccgcgt tccgacggcc cgctcctcag gacgcgcggg
      241 gatcgctggg cccgccacgg gttcccttgt gcgcctcttc gatgatccgc acgttgctga
      301 tcaccaccgt gtcggccatc agcaggtcga gggaggtcac gaacagccgc ccgatctcct
      361 cgtccgtgat ctcaggaccc gggatgtcga cgcggaaccc ctgggcctgc agtccgccgt
      421 cgttcgagaa cgtgatctcg gcgtcgaaca ctgcgcggta gctcgtcatc aggcctcgag
      481 ggccttgagc agctcgtcgg gcgccgcctg catggggtgg ggcccggcga tgtcgaggaa
      541 caccgtggtg atggtctcgc ggtgcgtggt caggaacgcc cgcagccatg ccggggagta
      601 gatgcccacc ccgggcggca ggttcgcggg cttgtgcttc gcgtcgctga acagcagcag
      661 cgccacctcc cctgtgcccg cgtcacggta ggtccagacc tcgccgccgt cgagcgggtt
      721 gtcgcgcggg ccgggcttga tcagggggac gaccgtgttg ccgttgcgca gcgccagggc
      781 gacggctgcc atgtcctgct tctcgagcgc ctgcgcgaga gcctcggagc ggaaatcctg
      841 gggtgcgggg cgcttcgcgc cggccctctt cttcgccatg gcctccagcc taggagacgc
      901 ggcctgagag ggaccgtcgg ctggacagag tggggccctc tcgaatcctc cattggggac
      961 tggggtactc gagagggccc tctggctctc accacgatga ttgtcgaagc gctggggacg
     1021 ctgataccga caccactggg gatggcttca ccgtattgtt cggagaacca cactccatgg
     1081 tatcccaaaa tactggtccg cggggcggaa gttcagaccc gatccacgga gagcacccgt
     1141 atcagcgcag gatgatctgc cgcgtgctcg cactggcgaa gccgccgatg gagacctcga
     1201 catggtacga ggccccgccg cccggggcgc gtgcacggtt ctgctgatcg caggtgccca
     1261 ccgccgagcg agtgcggtcc cacacgaccg gcgtcttggt gctcacggtc gcgcccgccg
     1321 tgagcgtcgc gatctgatcg ctcggatcgg tctggcagtc cgtcgaacgc caccagacgt
     1381 cgttgccgct cgtgatcgtg aacttctgcg tcgccgaccc gacgttcatc gtgcagtcgc
     1441 ggtcgctctt gttggtgagc gtgatcgaca gcttcggcag cgcgtccgcg gcataggtct
     1501 cggcatccgt caccgcggtc acctcgacat cgccggcttc gcaggcgacc acgcccggag
     1561 gcgccgacgg gtcgccggac ggatccgggc tcgccgagtc cgacggcgtc gcgggcggag
     1621 catccgtcgc cgtcccgtcc gtcggctgag cggacggcgt cttcggcgcc ggctccgcac
     1681 ccgtcttcca cggctgcgcg atggccaccc acagtccgcc cgtgaccacg gcgagcacca
     1741 cgatgaggat cagcaggacg gcgagtctcc ggcggcgata gacggcggcg gactgacggg
     1801 gcatgcttcc aggctagacg aggtgcttga gcatcctggt gttgccgagc gtgttgggct
     1861 tgacgtgcgc gaggtcgagg aactcctcca cgccggcgtc gccgctgcgc agcagctgcg
     1921 agtacacgtc ggggtcgacg atgtgctcgc cgatcggctc gaacccgcgg cgggtgaaga
     1981 agcccgtctc gaaggtgagg cagaacagcc gggagaggcc gagctcccgc gcacgctgct
     2041 ccagcgcttc gacgatcgcg cggccgacgc cgtgatgcag gaagtcgtcg cgtacgagca
     2101 gcgtgcgcac ctcgccgagg tcctcccaca tgacgtgcag ggcaccgcat ccgatgagct
     2161 ctccgtcgac ctcggcgacg acgaactcct gcacggcccc gtagaggatc gcgaggtcct
     2221 tcccgagcag gatgcgtcgc tcgaccagcg gctccagaag tgcgtggatg ccgttgatgt
     2281 cggcggtgcg ggccgcgcgg agggtgtact cgctcacgct tccagactag gacgccgtcc
     2341 gccggaacct gcgaagggct cggatgccga ggcatccgag cccttcaccg ctgcagcgag
     2401 tgcgtcagac cgtgacgatg acgtcgtcgc cctcgacgcg caccggcagg cggcgcagac
     2461 ccacgctctg atcggcggga tgcttgcccg tgaggatgtc gaactcgaag ccgtgccagg
     2521 ggcagacgat gttccggtcg ctcgggtggt gccccgggcc gccgctcttg aggccgccct
     2581 ccccgcggac gagctgcacc gtgcgcggca tgatcttgcc ctggcacacg ggtccgccct
     2641 ggtgcgggca gacgttgtac caggcgcgca cctcgccgtc gtggaagtag acgccgaact
     2701 ccgtgccgtc gacgtcgaag acgatgcgct cgcgctcctt caggtcggtc aggctcgcga
     2761 ccaccacgtc gcgacgcacg atctgagcgc tcattccgct ctccggtacc agatcgccat
     2821 gtcgaagagg ttgacgtccg cggcatccag cagcgccgtc accagcacca cggtctcggt
     2881 ggcgttcagc gcgtcctcgc ggatcggcca ggcgatcttg ccgacccgat cccgcttcgc
     2941 ggagaacagc cgggcggccg cgtacaggat gtcggcgatc tgctcgtcgg gcacctcggc
     3001 gccgagctcc tgctcggtga cgtggtgggt cagctccccg agccgggcga cgaggccctc
     3061 aagctcggcg agctcagcgg tggtggtgtt cgtttcagtc atcacgagat cccaggtcgg
     3121 tcctgcagca cctgctctgc agaaggcttc tgtcggtcgg tcgggagatt gaacacgcgt
     3181 gcggcgttca gccccaggat gttgcgcttg gcctgctcgg agaggaacgg caggcgcgtg
     3241 atcgtcgacg gcgcatccca gtcccagtgc ggccagtcgg acgagtagag cagctgggtc
     3301 tcagcattga acgacttcat ggtcgcctcg agcagctcca tgttggtgcg ctccatcggc
     3361 tgcgtcgtgt accacatctc cttcatgtac tcactcggga ggcgcttgag gccgggggcc
     3421 tcgctcgggc gcatgagcac ctcgtggtcg agccgctgca tgaggaacgg gatccacgcc
     3481 aggccgctct ccacccagac catcttgagc ttggggaagc gctcgggcat cgcgttgatg
     3541 atccagttgg tgacgtggat ctggttgtag tgcgtgaacg acagcgcgtg catcgacagg
     3601 aagcggttca gctgggagaa cgacgggtcg ccccagtgat agccggagtg gaaggcgagc
     3661 ggcttcccgc tgtcctcgat gagcttgtag agacgggtgt acacgtcgga gtgcaccggg
     3721 ttgttccggg tggagcacac ggtgtagccg atgatccccg ggtcgtccgc gtacttctcg
     3781 acgagctcct cgcacacctc gggggtgttg taaggcaggt acagcatgcc cttcaggcgc
     3841 gggttcggcg ggaggatgac ctccgtgagc cagcggttga acgcccggcc gaggacgacc
     3901 tcgacctcgt cctgcggatg catgccgagc agcagcatcg cgctcgggaa gacgacctgg
     3961 tagtccaggc ccatggcatc catcccgcgc cgggccgcgg tgacgaacgg atgcccgtcg
     4021 tcgggaccga ccggctcctt gcggccctgg tggccgatcc ggccgccgac gctctggtgc
     4081 gacatgccgg gctggatgtt cagcagggcg ttgccgccgc cgaagccggc gtactgctgg
     4141 aggccgatct gcttccagac gtcgttgtcg atgaggtcga gcacctcttt ccagaagtgg
     4201 tcctcggaga cgtgcgcgtc gatgtcgacg atgaagtagt cgtcgtagct ctcctgcgcc
     4261 tgcttggtgg cgtgtgcgag cacatcgcgc gtgtcggagc gtccgccgat ctcggcgagc
     4321 tcgcggatgc gaggcgcgtc ggagcggggg gtgggcagat cactcatggt ttcctctcgg
     4381 gatcggtcgg tcaacggggc gagacgatcg cattggtcgc gacggtgagg tattcgctct
     4441 cgctctgctg cttctgctgc gagatcacga ccgccctcgc cggcgcgacc gcgaacgaca
     4501 gctgctcgcc ctcgcgcggg atctcgtcgg cgctcagctc ggagatctcc acccggacgc
     4561 gcagcgggat gccgtcggcg cgcacgagca ggttgacggc gctgccgtga tcagcgtacc
     4621 ggtcagcatc gggaacgcag ttgattccca ggcaaaactg gccgcgcgca ggcggtcg
//
LOCUS       DATMSX010000170        10892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_182695,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000170 DATMSX010000000
VERSION     DATMSX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182695"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1444
                     /locus_tag="VN041_05200"
     CDS             245..1444
                     /locus_tag="VN041_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU28476.1"
                     /translation="MDVIPGAWRRTAAGAGLLSPQGEVAPTIFAEMSAAAVRFDAINL
                     GQGFPDEDGPSVVLDAAKDAIAHGANQYPPGRGIPDLRLAISEHQRRFYGLETDPDTE
                     VLVTAGATEALTATLLALIDSPEDEVVVFEPYYDSYAAVVALAGARLRTVPLRLPDFQ
                     PDLDQLAAVVNDRTRIILVNDPHNPTGAVFGPEVQAEVVRLANRHDAVIVTDEVYEHL
                     SFSAPHVPIATLPGAAERTLTISSGGKTFSATGWKIGWVHGPAELITAVLTVKQILTY
                     VNGSAFQPAIAVGLRLPDSYFDDARAMLLHKHGILGDALRTAGFDVHPPQGGYFTVAD
                     ATALGGADAMAFCRALPERARVVAIPITAFVAPEHHGDYSGLVRFAACKRVEVLEEAA
                     RRLAALG"
     gene            complement(1627..2433)
                     /locus_tag="VN041_05205"
     CDS             complement(1627..2433)
                     /locus_tag="VN041_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWU28477.1"
                     /translation="MTASAPLVAVCQFAPTESRESNRARIAGLVADAAARGARVIVLP
                     EYSSFFVDPMDDSLAANAEPVGGEFVQTLQSLAGEHGAVIVAGMVETADAGRVHNTLV
                     AVSDAGVQAVYRKQHLYDAFGQTESDWIQAGELGQASVFDVDGIRFGMMTCYDLRFPE
                     VARTLVDAGADALIVPAEWVRGPLKEHHWGTLLTARAIESTAYVIAADHPAPIGVGLS
                     QVIDPQGVAVAGVSAGEGIALASVDPGLIARTREANPVLRLRRYAVVPQR"
     gene            complement(2454..3881)
                     /locus_tag="VN041_05210"
     CDS             complement(2454..3881)
                     /locus_tag="VN041_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWU28478.1"
                     /translation="MALGSAIGTGLFYGSSAAIQTAGPAVLLAYMIGGAVIFLVMRAL
                     GEMAVRHPVAGSFGQYASRYVGPFAGFLTGWTYTFEMFVVALADVTAFGVYMGFWFPQ
                     VERWVWILAVVFIIAAVNLVSVKVFGELEFWFALIKIVAIVAMIAGGIAIILFGFANA
                     GGHEAGFHNLVGEGGFAPNGLWGVLASLTIVMFAFGGTEVIGITAGEAENPKKVLPRA
                     INSVPVRILLFYVLALGVIMMIQPWDTIDGEASPFVSIFSSLGLAGAANILNLVVITA
                     ALSAINADIFAAGRMLHGLAEQGQAPRAFARTTKAGVPFMTVLTMVAALLVGVLLNVW
                     FHDQIFFLIAALATFATVVVWLMILVSHARMKAEITREKKLPSEFPAPLWPVGNYVAM
                     AVIALVIVLIGIIPDSRPALVVGAIWIALLAAAYFGLVRGTGRLRKELVDETAAIPVI
                     RVEQESASEDADDAVDEEGEPERMR"
     gene            complement(4164..4237)
                     /locus_tag="VN041_05215"
     tRNA            complement(4164..4237)
                     /locus_tag="VN041_05215"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4201..4203),aa:Met,seq:cat)
     gene            complement(4334..4534)
                     /locus_tag="VN041_05220"
     CDS             complement(4334..4534)
                     /locus_tag="VN041_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HWU28479.1"
                     /translation="MRAEVGSRIRIHGARVGNSERHGEVLETRGEDGGPPYLVRFDDG
                     GESLIFPGPDCELERQGDADDA"
     gene            complement(4702..7614)
                     /locus_tag="VN041_05225"
     CDS             complement(4702..7614)
                     /locus_tag="VN041_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWU28480.1"
                     /translation="MTTSSSHPPATRSASRRILAVSVAIIVALIAAFFIFASLYTEYL
                     WFDQLKFTNVLTTQWIATVSMFGLGFLGMALPIFLCIQLAYRLRPVYVRLSSQLDRYQ
                     EVIEPLRRLAMWGMPVFFGIFAGFAAAGNWKTVWLWANGVSTKTLDPEFHLDTGFYLF
                     AMPFYSMLLAFVSAVLLLCLVITALVSYLYGSVRIGQRELRISKPARIQLAVLAGLYL
                     AVQAVSLWLDRYLTLVQPEGRITGAGYTGVNATIPGLAILSIIAAVVAVLFLITAVIG
                     RWRFPLAATALLIVASLVVGMGYPWVVTTFQVKPNENSYQAQYYERNINATKEAFGVA
                     DMEVTPFKATTDTAAGQLRADADTTASIRIMDPAVIGPTVRQLEQYRSYYQFATDMDV
                     DRYTIDGKKQDTVVSVRDLDMNKLGGGDSWNNRAAVYTHGYGLVAMAGNERTSDGDPV
                     FLEQGIPTSGFLTDSEHFEPRVYFGETSPEYSIVGAPEGTKPVEIDYPRGQDSESSDT
                     KTTFTGNGGPRIGNTFTKLLYALKFQSEQILFSNLVNPDSQILYDRDPLTRVKKVAPY
                     LTLDRDPYPSVVDGHIVWIVDGFTTSASYPYSKTVSMQDAIADSQTPASAVAFDNINY
                     IRNSVKATVDAYDGSVKLYAWDDQDPVLKAWEKIYPTTVKPISDMSADLISHVRYPTD
                     LFKVQRDILGVYHIDDAGSFAQEDNRWQTPEDPREKGQLQPPYYLSMKMPGQDEPRFS
                     MFSTFIPADGAGDSRQVLMGYLAVDSDAGDKAGVKAKDYGKLRMLEIDTSSTVPGPGQ
                     VQNTFDSNASVAEKLNVLTIGKSEVKYGNLLTLPVGGGLLYVQPVFVQSSEGTKLPTL
                     RKILVAFGDRVAFEDTLTEALDALFGGDAGAPGGDEGVTPNPGDGGTTPPDEGGTTPP
                     TSSDAMKQALADAGAALAARNAALRDGDLSEFAVQDEKLTAAVQKILELEGQTAGE"
     gene            complement(7675..8769)
                     /locus_tag="VN041_05230"
     CDS             complement(7675..8769)
                     /locus_tag="VN041_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWU28481.1"
                     /translation="MSSRRSNGTIVGVVALSISLVALVGLTFIPTQYVIQRPGPVFDT
                     LGTAKGTDGASVPLIEVKDAKTYPTDGTLDLTTVQVIGNRQRTPTWFELALAWFDPSR
                     AVVPIDAVFPEGVTSEQRDQQNAALMVDSQHEATAAALTELGYDVGAQVEVVSVLKDS
                     PAEGLLKDGDRILAIDGVDVNSATLLRDTIQKAKGSEVTLSVKRGSETDTVAITPKKT
                     KDASGATTWLIGITLTTDYTFPVDVKLQLDNVGGPSAGMMFALGIIDTLTPGELNGGV
                     NVAGTGTIDAAGDVGPIGGIRQKLFGARDAGATVFLAPESNCDEVVGHIPDGLKVFKT
                     ATLQDSLDILEVIADHGDTSKLPVCTASSK"
     gene            8888..10351
                     /locus_tag="VN041_05235"
     CDS             8888..10351
                     /locus_tag="VN041_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWU28482.1"
                     /translation="MTDDDRDPSDFEEMLRRMMSGGQIDPEELREALSNMQGMPIDPS
                     QMQGFMSQMQGMFAGDPWQNAERAALHIANQDGLGITAGSQSSLADAFGLANLWLSEA
                     TTISELSTTPQAMTRGDWVQKTLPVWKEIATPVSTSIADALMDALQTQVPEEMQGMVQ
                     GAGQLMRGLGGSLFATQFGQVLGRLSLEVVSGGDVGIPVLPAGTAAVIPQNIAAFGEG
                     LEIPEDQITLYLAVRELAYARLYRHAKWLHLHVLSQITDFARGVTVDIDALEDLASRL
                     DPSNPEELRAAIEGGALLPAQTDAQREALTRLENLIATIDGWVDVVTGQAIARLPDGA
                     RLAEAARRRRAVGGPAEDALGALVGLKLRPRRLREASAMWKAVTDAVGVAARDSLWDY
                     PDLMPSSEDIDDPTALIERLQALQSGEVPAADEFDEALARLLDGDDFGRAPGENGEAP
                     GADGDAPGNGREISDGDTGADGSDPASDDRPEGDRPV"
     gene            10470..>10892
                     /locus_tag="VN041_05240"
     CDS             10470..>10892
                     /locus_tag="VN041_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28483.1"
                     /translation="MSPRPTPATTRLDARYPLLWRDERTVQFGLEGVVAIDVDEPWVE
                     PLLLRLQAGIRPASFDVVAHGVGAPRVAARELLSRLRPVLVSDPAPAPPVSIEGVNLS
                     DDRALERMRQAIEDEGIAVADPDAPDAVALVLLEGAAAR"
BASE COUNT         1704 a         3714 c         3750 g         1724 t
ORIGIN      
        1 gtacgcgccg gtggcgggct gaccggtctg aggctggtcg gccgcggcgg gcgcacccgg
       61 tgcggagaac ggagggttcg cgggcgtcga gccggcgttc ggcatcccgg ggcccgggac
      121 ctcaccgttg gtgttgttgt cgttcatgat ctgctccttc caggcgcacc atcagactga
      181 caagcgtccc tgtggttttc atatggcgaa gttggatttc tcctatggtc ttagcctgtt
      241 ctgcatggat gtcattcccg gcgcctggcg gcgcaccgca gcaggagcag gtctgctctc
      301 cccacagggc gaagtcgcgc ccaccatctt cgcagagatg tctgcggcgg cggtccgatt
      361 cgacgcgatc aacctcgggc agggcttccc cgacgaggac ggcccgtcgg tggtcctgga
      421 tgcggcgaag gatgccatcg cgcacggcgc gaaccagtac ccgccggggc gcggcatccc
      481 ggacctgcgg ctcgcgatca gcgagcacca gcgccgcttc tacggactgg agaccgatcc
      541 cgacaccgag gtgctcgtca ccgccggcgc caccgaggcc ctcacggcga ccctgctcgc
      601 gctgatcgac tcgcccgagg acgaggtggt cgtcttcgag ccctactacg actcctatgc
      661 tgcggtcgtg gcgctggccg gagcgcgcct gcgcacggtt ccgctgcggc tccccgactt
      721 ccagccggat ctggatcagc tcgccgccgt cgtgaacgac cgcacccgca tcatcctcgt
      781 gaacgaccct cacaacccga ccggtgcggt gttcggcccc gaggtgcagg cggaggtcgt
      841 gcgactggcg aatcggcacg acgcggtcat cgtgaccgac gaggtctacg agcacctgtc
      901 gttctcggcg ccgcacgtgc cgatcgcgac gctgccgggc gccgccgagc ggacgctgac
      961 catctcgtcg ggcggcaaga cgttctcggc gaccggctgg aagatcggct gggtgcacgg
     1021 tcccgccgag ctgatcaccg cggtgctcac cgtgaagcag atcctcacct atgtgaacgg
     1081 ctcggcgttc cagccggcga tcgccgtcgg tctgcgactg ccggactcct acttcgacga
     1141 cgcccgggcg atgctgctgc acaagcacgg catcctgggc gatgcgctgc gcacggcagg
     1201 attcgacgtg cacccaccgc agggcgggta cttcaccgtc gccgacgcga cagcgctggg
     1261 cggcgcggac gccatggcct tctgccgcgc acttccggaa cgcgcccgcg tggtggcgat
     1321 cccgatcacc gcgttcgtgg ctcccgagca tcacggcgac tattcggggc tggtgcgctt
     1381 cgccgcctgc aagagggtcg aggtgctcga ggaggccgcc cgccggctcg cggcgctcgg
     1441 ctgagggccg ggcatcgggc atccggcatc cggcatccgg cttgacgacg cgctccgggg
     1501 gccgatggac cggtatcccg atgcgctccg ggggccgaca cgccagattt cggacctttc
     1561 ccgcgaaccc gtccgtcatc tgacgtgtcg gcccccgttc tgtatcgcgc gaacggcgat
     1621 gggagctcag cgctgcggga ccacggcata gcggcgcagc cgcaggacgg gattggcctc
     1681 acgcgtccgg gcgatcagcc ccgggtcgac ggatgccagt gcgatgccct cccccgcgct
     1741 gacgccggcg accgcgaccc cctgcgggtc gatcacctgc gacagaccga cgccgatcgg
     1801 tgcgggatgg tcggcggcga tcacgtacgc cgtgctctcg atggcgcgcg cggtgagcag
     1861 cgtgccccag tgatgctcct tgagggggcc gcgcacccac tcggcgggca cgatcagcgc
     1921 atccgctccg gcatcgacga gggtgcgcgc gacctccggg aagcgcaggt cgtagcaggt
     1981 catcatcccg aaccggatgc cgtcgacgtc gaacaccgac gcctggccga gctctccggc
     2041 ttgaatccaa tcggattcgg tctgcccgaa cgcatcgtag aggtgctgct tgcggtacac
     2101 cgcctgcact ccagcatccg acaccgcgac cagcgtgttg tgcacacggc cggcatccgc
     2161 tgtctcgacc atgcccgcga cgatgacggc gccgtgctca cccgcgagcg actgcagggt
     2221 ctgcacgaac tccccaccga ccggctctgc gttcgcggcg agcgagtcgt ccatcgggtc
     2281 gacgaagaag ctgctgtact ccgggagcac gatcacccgg gcaccgcgag ccgcggcatc
     2341 tgcgacgagt cccgcgatgc gcgcgcgatt ggactcccgc gattcggtgg gcgcgaactg
     2401 gcagacggcg acgagcgggg cggatgcggt catctgccca tcctcgcgca tcatcagcgc
     2461 atgcgctcgg gctcgccctc ctcgtcgacg gcgtcgtccg cgtcctccga cgcggactcc
     2521 tgctcgacgc gaatgacggg gatcgccgcc gtctcgtcca cgagctcctt gcggagccgg
     2581 ccggtgccgc ggaccaatcc gaagtaggcc gcggcgagca gggcgatcca gatcgctccg
     2641 accacgagcg ccgggcggct gtcggggatg atgccgatca gcacgatgac gagcgcgatg
     2701 acggccatcg ccacgtagtt cccgaccggc cagagcggtg ccgggaactc ggagggcagc
     2761 ttcttctcgc gggtgatctc tgccttcatc cgggcgtgcg acacgaggat catcagccag
     2821 acgacgacgg tcgcgaaggt cgccagcgcc gcgatgagga agaagatctg gtcgtggaac
     2881 cagacgttca gcagaacccc gaccaggagg gccgcgacca tggtgagcac ggtcatgaac
     2941 ggcacgcccg ccttggtcgt gcgcgcgaac gcgcggggag cctggccctg ctccgccagc
     3001 ccgtgcagca tgcgtccggc ggcgaagatg tcggcgttga tggccgacag cgccgccgtg
     3061 atgacgacga ggttcaggat gttcgccgcg ccggcgagac ccaggctgct gaagatcgac
     3121 acgaacggac tcgcctcgcc gtcgatcgtg tcccacggct ggatcatcat gatcacgccg
     3181 agcgccagca cgtagaacag caggatgcgg accggaacag aattgatggc gcgcggcagc
     3241 accttcttcg ggttctccgc ctcgcccgcg gtgatgccga tgacctcggt gccgccgaac
     3301 gcgaacatca cgatggtcag cgacgcgagc acgccccaga gcccgttcgg cgcgaagcct
     3361 ccctcgccca ccaggttgtg gaaccccgcc tcatgtccgc ccgcgttcgc gaagccgaag
     3421 aggatgatcg cgatgccacc ggcgatcatc gcgacgatgg cgacgatctt gatcagggcg
     3481 aaccagaact ccagctcgcc gaacaccttc acgctgacga ggttcaccgc cgcgatgatg
     3541 aacaccaccg cgaggatcca cacccagcgc tcgacctgcg ggaaccagaa ccccatgtac
     3601 acgccgaagg ccgtcacgtc ggcgagggcg acgacgaaca tctcgaaggt gtaggtccag
     3661 cccgtcagga agccggcgaa ggggccgacg taccgcgagg cgtactgccc gaaggatccg
     3721 gccacgggat ggcgcacggc catctcaccg agggcacgca tcaccaggaa gatgaccgcg
     3781 ccgccgatca tgtaggcgag cagcaccgcc gggcccgcgg tctggatcgc ggcggacgag
     3841 ccgtagaaca ggccggtgcc gattgcggat cccagagcca tgaactggat gtgccgggcg
     3901 gtcaggccac gacgcagcgt ctgcgaggtc tcggtggtgt gcacgattct ccttcgaacg
     3961 gaccggccga tcgcgtgatc cgggcttcat cacgacgccc gaaccgtgcg gatccctacg
     4021 atgaaatccc atcggcgggt cgctccccga ccagatgcgg gaatctgcgt ctgggatcac
     4081 agacgatcgg ccgtccctgc gcgatcggac gccgcagacg cgaagaaggc ccgatccttg
     4141 cggaccgggc cttcttctct ttgttgcggg gacaggattt gaacctgcga cctccgggtt
     4201 atgagcccgg cgagctaccg aactgctcca ccccgcggca cgtcttaaag cctaacacgc
     4261 aggtgaacag gaggcgaatc gagcggcctc ccccgcgacg gacgggcgtg tcgcgccccc
     4321 gtcggccgtg cgttcaggcg tcgtcggcgt ccccctggcg ctccagctcg cagtccgggc
     4381 cggggaagat cagcgactcc ccgccgtcgt cgaaccgcac cagatagggc ggtccgccgt
     4441 cctccccgcg cgtctccagc acctcgccgt gccgctccga gttcccgacc cgtgcgccat
     4501 ggatcctgat gcgcgatccc acttctgctc gcatcgcgac cacctcccgc agcctgatgc
     4561 tctcattgtc cgcgcgtccg agccggactg ccagtggcag tctgcagtcg ggcatcagcg
     4621 gcagaacttc ggagaaatgc agaatctcgg aggcatccga gtggattcct ccgagattct
     4681 gcatatctcc gcagatgcgg gctactcccc cgcggtctga ccctccagct cgaggatctt
     4741 ctggaccgcg gcggtgagct tctcgtcctg cacggcgaac tcggagaggt cgccgtcgcg
     4801 cagcgccgcg ttcctcgcgg cgagcgccgc tccggcatcc gccagcgcct gcttcatggc
     4861 atccgaggac gtgggaggcg tggtgccgcc ctcatccggc ggcgtggtgc ccccgtcccc
     4921 gggattcggg gtcacgccct cgtcgccgcc cggcgcaccg gcgtcgccgc cgaacagcgc
     4981 gtccagcgcc tcggtcaggg tgtcctcgaa cgcgacccgg tcaccgaagg cgacgaggat
     5041 cttgcgcagc gtgggcagct tggtgccctc ggacgactgc acgaacaccg gctgcacgta
     5101 cagcagaccg ccgccgaccg gcagagtcag caggttgccg tacttcacct ccgactttcc
     5161 gatcgtgagc acgttcagct tctcggccac gctggcgttc gagtcgaacg tgttctgcac
     5221 ctgaccgggg cctggcacag tgctggaggt gtcgatctcc agcatccgca gcttgccgta
     5281 gtccttcgcc ttcacgcctg ccttgtcgcc cgcgtccgaa tcgacggcca ggtagcccat
     5341 cagcacctgc cggctgtcgc cggcgccgtc ggcggggatg aacgtcgaga acatcgagaa
     5401 ccgcggctcg tcctgacccg gcatcttcat ggacaggtag tacgggggct gcagctggcc
     5461 cttctcgcgc gggtcctccg gcgtctgcca gcggttgtcc tcctgagcga aggagcccgc
     5521 gtcgtcgatg tggtagacgc cgaggatgtc gcgctgcacc ttgaacaggt cggtggggta
     5581 ccgcacgtgg ctgatcaggt cggccgacat gtcgctgatc ggcttgaccg tggtcgggta
     5641 gatcttctcc caggctttca ggaccggatc ctggtcgtcc caggcgtaca gcttgacgga
     5701 gccgtcgtag gcgtccacgg tggccttgac cgagttgcgg atgtagttga tgttgtcgaa
     5761 cgcgaccgcg gaggccggcg tctgcgagtc tgcgatggcg tcctgcatgc tgaccgtctt
     5821 ggagtacggg tacgacgcgc tggtggtgaa gccgtcgacg atccacacga tgtggccgtc
     5881 cacgacgctc gggtaggggt cgcggtccag cgtcaggtac ggggcgacct tcttcacgcg
     5941 cgtgagcggg tcgcggtcgt acaggatctg cgagtcgggg ttgaccaggt tcgagaacag
     6001 gatctgctcg gactggaact tcagcgcgta cagcagcttg gtgaacgtgt tgccgatccg
     6061 cggtccgccg ttgccggtga acgtggtctt ggtgtcgctg ctctcgctgt cctgaccgcg
     6121 cgggtagtcg atctcgacgg gtttggtgcc ctccggcgcg ccgacgatgg agtactccgg
     6181 cgaggtctcg ccgaagtaca cgcgcggctc gaagtgctcg ctgtcggtca ggaagcccga
     6241 agtggggatg ccctgctcca ggaacaccgg atcgccgtcc gaggtgcgct cgttgcccgc
     6301 catggcgacc aggccgtagc cgtgcgtgta caccgcagcg cggttgttcc agctgtcacc
     6361 gccgccgagc ttgttcatgt cgagatcgcg gaccgagacg acggtgtcct gcttcttgcc
     6421 gtcgatcgtg tagcggtcga cgtccatgtc cgtggcgaac tggtagtagg agcggtactg
     6481 ctcgagctgg cggacggtcg ggccgatgac ggccgggtcc atgatgcgga tggatgccgt
     6541 ggtgtcggca tccgcacgca gctgaccggc ggcggtgtcg gtggtcgcct tgaacggagt
     6601 gacctccatg tccgcgacgc cgaacgcctc cttggtggcg ttgatgttcc gctcgtagta
     6661 ctgcgcctga tagctgttct cgttcggctt cacctggaag gtggtgacaa cccacgggta
     6721 gcccatgccc accaccagcg aggcgacgat cagcagcgcg gtggcggcca gcgggaagcg
     6781 ccagcggccg atcacggcgg tgatcaggaa cagcacggcg acgaccgcgg cgatgatcga
     6841 caggatcgcc agtcccggga tcgtggcgtt cactccggtg tatcccgcac cggtgatgcg
     6901 gccctccggc tgcaccagcg tgaggtagcg gtccagccac aggctcacgg cctgcacggc
     6961 gaggtagagg ccggcgagca ccgcgagctg gatgcgcgcg ggcttggaga tgcgcagctc
     7021 gcgctggccg atgcgcaccg agccgtacag ataggacacc agcgcggtga tcaccaggca
     7081 cagcagcagc acggcagaga cgaacgccag cagcatcgag tagaacggca tcgcgaagag
     7141 gtagaagccc gtgtccaggt ggaactcggg gtcgagcgtc ttggtgctca caccgttggc
     7201 ccacagccac acggtcttcc agttgcccgc ggcggcgaag cccgcgaaga tgccgaagaa
     7261 caccggcatc ccccacatcg ccagccggcg cagcggctcg atgacctcct gatagcggtc
     7321 cagctgcgaa ctcagccgca cgtacaccgg gcggagccgg tacgcgagct ggatgcacag
     7381 gaagatcggc agcgccatgc cgaggaagcc gagcccgaac atcgagaccg tggcgatcca
     7441 ctgcgtggtc aggacgttcg tgaacttcag ctggtcgaac cagaggtact cggtgtacag
     7501 cgacgcgaag atgaagaacg cggcgatcag ggcgacgatg atcgccaccg agacggcgag
     7561 gatccggcgc gaggcgctgc gtgtggccgg cgggtgggat gaggaggtgg tcaccgtccc
     7621 atcctatgca gggcacctat gacggcaccc cgggttccgc tcagcgcgaa gacgtcactt
     7681 cgatgatgcg gtgcacaccg gcagcttcga ggtgtcgccg tggtcggcga tgacctcgag
     7741 gatgtcgagg gagtcctgca gcgtggccgt cttgaacacc ttcagcccgt ccgggatgtg
     7801 gccgacgacc tcgtcgcagt tgctctccgg ggcgaggaag accgttgcgc cggcatcgcg
     7861 cgcgccgaac agcttctgac ggatgccccc gatcggcccg acatcccccg cggcgtcgat
     7921 cgtgccggtg ccggccacgt tcactccgcc gttgagttcg cccggcgtga gcgtgtcgat
     7981 gatcccgagc gcgaacatca tgccggcgct ggggccgccc acgttgtcga gctgcagctt
     8041 gacgtcgacg gggaaggtgt aatcggtcgt gagggtgatc ccgatcagcc aggtagtggc
     8101 gccactggca tccttcgtct tcttcggggt gatcgcgacg gtgtcggtct cgctcccccg
     8161 cttcacgctg agcgtgacct cggagccctt cgccttctgg atcgtgtcgc gcagcagggt
     8221 ggccgagttc acgtcgaccc cgtcgatcgc gaggatccgg tcgccgtcct tcagcaggcc
     8281 ctccgcgggc gagtccttga gcacgctgac gacctcgacc tgagcgccga cgtcgtagcc
     8341 cagctcggtg agggccgccg cggtcgcctc gtgctgggag tcgaccatca gcgcggcgtt
     8401 ctgctgatcg cgctgctcgc tggtcacgcc ctccgggaag acggcgtcga tcggcacgac
     8461 ggcacgcgag gggtcgaacc aggccagtgc cagctcgaac caggtgggcg tgcgctgcct
     8521 gttgccgatg acctgcaccg tggtgaggtc gagcgtgccg tcggtcgggt acgtcttggc
     8581 gtccttcacc tcgatgagcg ggaccgaggc gccgtcggtg cccttggccg tgccgagcgt
     8641 gtcgaacacc gggccgggcc gctggatcac gtactgggtc gggatgaagg tcagtccgac
     8701 cagcgcgacc agcgagatgc tcagtgcgac gacgccgacg atcgtgccat tgctgcggcg
     8761 tgatgacacg tgacctccgg gcgttcgcga gcggcgtaca gatatccccg ctgccagggc
     8821 tggagcacag gatcgtgtga ctagcgtaga gcgcacacgc tacgggcatg ctgagagggc
     8881 cattcgcatg acagacgacg accgcgatcc gtccgacttc gaggagatgc tgcgccgcat
     8941 gatgtccggc gggcagatcg acccggagga gctccgcgag gcgctctcga acatgcaggg
     9001 catgccgatc gatccctccc agatgcaggg cttcatgtcg cagatgcagg gcatgttcgc
     9061 gggtgacccg tggcagaacg ccgagcgagc cgcgctgcac atcgccaacc aggacggtct
     9121 cggcatcacc gcaggttccc agtcgtcgct ggcggatgcc ttcggcctgg cgaacctgtg
     9181 gctcagcgag gccaccacga tctcggagct gtccacgacc ccgcaggcga tgacccgcgg
     9241 cgactgggtg cagaagaccc tgccggtgtg gaaggagatc gccacgcccg tgtcgacctc
     9301 gatcgccgac gccctcatgg acgcgctgca gacgcaggtg cccgaggaga tgcagggcat
     9361 ggtgcagggc gcggggcagc tgatgcgggg cctgggcggt tcgctgttcg ccacccagtt
     9421 cgggcaggtg ctgggccggc tgtcgctcga ggtcgtctcc ggcggagacg tgggcatccc
     9481 ggtgctgccc gccggaacgg cggcggtcat cccgcagaac atcgccgcgt tcggcgaggg
     9541 gctggagatc cccgaggacc agatcaccct ctacctcgcg gtgcgcgagc tggcctacgc
     9601 gcggctgtac cggcacgcca agtggctgca cctgcacgtc ctctcgcaga tcaccgactt
     9661 cgcccgcggt gtgacggtcg acatcgacgc tctggaggac ctcgcctcgc gactggaccc
     9721 ctcgaacccg gaggagctgc gcgcggcgat cgagggcggt gccctgctgc ccgctcagac
     9781 ggatgcccag cgcgaggcgc tcacgcgcct ggagaacctc atcgccacga tcgacggctg
     9841 ggtcgacgtc gtcaccggac aggccatcgc acggctgccc gacggtgccc gtctcgccga
     9901 ggcggctcgc cgtcgtcgcg cggtcggcgg ccccgccgag gatgccctgg gtgcactggt
     9961 cggcctcaag ctgcgtccac ggcgactgcg cgaggcgtcc gcgatgtgga aggccgtgac
    10021 ggatgccgtg ggcgtcgccg cccgtgactc gctgtgggac taccccgacc tcatgccgtc
    10081 atcggaggac atcgacgatc cgacggcgct gatcgagcgg ctgcaggcgc tgcagagcgg
    10141 cgaggtgccc gcggccgacg agttcgacga ggcacttgcg cggcttctgg acggcgacga
    10201 tttcggccgg gcgcccggcg agaacggtga ggctcccggt gccgacggcg atgcccccgg
    10261 caacggccgc gagatctcgg acggcgacac gggagcggac ggctccgacc cggcatccga
    10321 cgaccgcccg gagggcgacc gcccggtctg agtctccgtc ctccgtaccc gagccactct
    10381 ccctccacag ccggctcgtc ccgtggatcc gtccacagat ggtccggaca cggcccgcgg
    10441 atgccgcgga gtgcccacaa tcggggggta tgagcccgcg accgactccc gcgaccaccc
    10501 gactcgatgc tcgctatccg ctgctgtggc gggacgagcg caccgtgcag ttcgggctgg
    10561 agggcgtggt cgcgatcgac gtcgacgagc cctgggtgga accgctgctg ctgcggctcc
    10621 aggcgggcat ccgcccggcg tccttcgacg tcgtggcgca cggagtcggg gcgccccgcg
    10681 tcgccgcgcg cgagcttctc tcccggctcc gccctgtgct ggtgagcgac ccggcacccg
    10741 caccgccggt gagcatcgag ggcgtcaacc tgtcagacga ccgtgcactg gagcggatgc
    10801 ggcaggcgat cgaggacgag ggcatcgccg tggcggatcc ggacgccccc gacgccgtcg
    10861 ccctcgtcct gctcgagggc gccgcggcga ga
//
LOCUS       DATMSX010000171         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_134848,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000171 DATMSX010000000
VERSION     DATMSX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134848"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VN041_05245"
     CDS             complement(<1..914)
                     /locus_tag="VN041_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWU28484.1"
                     /translation="MTSTAPRTLAEKVWDDHLVVKGENGEPDLIYIDLHLIHEVTSPQ
                     AFDGLRTEGRPLRRVDLTIGTEDHNTPTLDIDKPIADLTSRTQIETLRRNAEEFGVRL
                     HSLGDAEQGIVHVVGPQLGLTMPGITVVCGDSHTSTHGAFGAMAFGIGTSEVEHVMAT
                     QTLPLKPFKTMAINVEGELRPGVTAKDIILAVIAKIGTGGGQGYVLEYRGSAIRALSM
                     EGRMTICNMSIEAGARAGMVAPDETTFAYVKDKPHAPQGQDWEDAVAYWRTLPSDPDA
                     VFDAEVDIDASTLEPFVTWGTNPGQGSSL"
     gene            complement(1091..2080)
                     /locus_tag="VN041_05250"
     CDS             complement(1091..2080)
                     /locus_tag="VN041_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HWU28485.1"
                     /translation="MDIPVWFEITSLVVLTAILIGDLLLILKRPHIPSTKESALWVVF
                     YVTLALIFAGVLFLIGDAKTSLDFLTGWAMEYSLSIDNLFVFILIMTQFSVPRRYQQE
                     ALMVGIIIALVLRAIFIIIVGAAVQHLSPIFYIFGAFLLYTAIRQALPDKEEDDAQTE
                     GFIVRQIRRVIPISDEYDGPKVRTTINGKKIWTPMLIVFVSLGVTDLMFAVDSIPAIF
                     GITTNAFIVFTANLFALMGLRQLYFLLGDLLDRLRYLHYGVAFILGFIGVKLVLHAMH
                     ENELSFINGGQPIEWAPDINNWVSLGVIFASMAVATIASLIASAREKRTPVAS"
     gene            2312..2869
                     /locus_tag="VN041_05255"
     CDS             2312..2869
                     /locus_tag="VN041_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU28486.1"
                     /translation="MPTSTEDREWAARAASGDQDAFREIYRAHVRPVYWIAHGILGSA
                     PDAEDVAQETFVVAWRKLPGLELAGDSLLPWLATICRFQAANRLRILRRDRANTADAL
                     EETIPDTISVEDQAISAALAERIAEEVGRLDETDRRIFRLCAVEGYAYQVAAEELGVS
                     HAVVRNRLSRVRTRLRGAAQEMRDA"
BASE COUNT          518 a          932 c         1085 g          504 t
ORIGIN      
        1 agcgagctgc cctgacccgg gttggtgccc caggtgacga agggctcgag ggtggaggcg
       61 tcgatgtcca cctcggcgtc gaacacggca tccgggtcgg aggggagcgt gcgccagtac
      121 gcgacggcat cctcccagtc ctgcccctgc ggtgcgtgcg gcttgtcctt cacgtaggcg
      181 aaagtggtct cgtcgggagc gaccatgccg gcccgggcgc ccgcctcgat cgacatgttg
      241 cagatcgtca tgcggccctc catcgagagg gcccggatcg cgctgccacg gtactcgagc
      301 acgtagccct gtcccccgcc ggtgccgatc ttcgcgatca cggcgagaat gatgtccttg
      361 gccgtgacac ccgggcggag ctcgccctcg acgttgatcg ccatggtctt gaacggcttc
      421 agcggcagcg tctgcgtggc catcacgtgc tcgacctcgc tggtgccgat gccgaaggcc
      481 atggcgccga acgcgccgtg cgttgacgtg tgcgagtcgc cgcagaccac tgtgatgccg
      541 ggcatggtca gccccagctg cggaccgacg acgtgcacga tgccctgctc ggcatcgccg
      601 agcgagtgca gtcgcacgcc gaactcctcg gcgttgcggc gcagagtctc gatctgcgtg
      661 cggctggtga ggtcggcgat cggcttgtcg atgtcgagcg tcggcgtgtt gtggtcctcg
      721 gtgccgatgg tcaggtcgac gcggcggagc ggacggccct cggtacgcag cccgtcgaag
      781 gcctgcgggc tggtgacctc gtggatcagg tgcagatcga tgtagatcag gtcgggttcg
      841 ccgttctcgc ccttgacgac gagatggtcg tcccagacct tctctgcgag ggtgcggggt
      901 gcggtgcttg tcatgcggtg tgttccttgt ggctcgatga gtcggcccat gatggactcc
      961 gcggcgagga aggcctagat cgaggtcccg ccgcggctgc taaggaggag aaccacacgc
     1021 cgcatgcggg ccagtctaca acgcggcatc gggtgcttcg cccgatgccg cgttgtgtga
     1081 cgatccgctc tcagctcgcg accggcgtgc gcttctcgcg ggcggacgcg atcaggctgg
     1141 cgatcgtggc gaccgccatc gatgcgaaga tcacgcccag cgacacccag ttgttgatgt
     1201 cgggcgccca ctcgatcggc tgaccgccgt tgatgaacga cagctcgttc tcgtgcatgg
     1261 cgtgcagcac gagcttgacg ccgatgaagc cgaggatgaa cgcgaccccg tagtgcaggt
     1321 agcgcagcct gtcgagcagg tcgcccagca ggaagtacag ctgacgcagg cccatcaggg
     1381 cgaacaggtt ggcggtgaac acgatgaacg cgttcgtcgt gatgccgaag atggcgggga
     1441 tggagtcgac cgcgaacatc aggtcggtca cgcccaggga cacgaagacg atcagcatcg
     1501 gcgtccagat cttcttgccg ttgatggtgg tgcgcacctt cgggccgtcg tactcgtcgc
     1561 tgatcgggat cacccggcgg atctggcgga cgatgaagcc ctcggtctgc gcgtcgtcct
     1621 cctccttgtc gggaagagcc tggcggatcg ccgtgtacag caggaaggcg ccgaagatgt
     1681 agaagatcgg gctgaggtgc tggacggcgg cgccgacgat gatgatgaag atcgcgcgca
     1741 ggaccagcgc gatgatgatg cccaccatca gcgcctcctg ctgatagcgc cgtggcaccg
     1801 agaactgggt catgatcagg atgaacacga acaggttgtc gatggagagg ctgtactcca
     1861 tcgcccatcc ggtgaggaag tcgagcgatg tcttggcatc gccgatgagg aacagcacac
     1921 cggcgaagat cagcgcgagc gtcacgtaga acacgaccca cagggccgat tccttggtgc
     1981 tggggatgtg cgggcgcttc aggatgagca gcagatcgcc gatgaggatg gcggtcagca
     2041 cgaccagcga ggtgatctcg aaccagacag ggatatccaa gggcggagga cctttcggag
     2101 ggttggcgga agtccgaaag tctctccccg caccggtggt gcgccgcgcg accggagcag
     2161 cgatgacggt gctcgtgatg acgatctgcg cgatgtcggg atactccctc tcgcagagcc
     2221 catgttaccg aacaatccgg gatgtcgagg aatctgagac gatgaggccg gacgcgacac
     2281 ttcctgatca acagacacgg acgggacggt catgccgacc agcacagagg atcgcgagtg
     2341 ggccgcacgg gcggcatccg gagatcagga tgccttccgc gagatctatc gcgcccatgt
     2401 gcggccggtg tactggatcg cgcacggcat cctcggttcg gcgccggatg ccgaggatgt
     2461 ggcgcaggag accttcgtgg tcgcctggcg caagctgcca gggctcgagc tggccgggga
     2521 ttcgctgctg ccctggctcg cgacgatctg ccgcttccag gcggcgaacc ggctgcggat
     2581 cctgcgccga gaccgggcca acaccgcgga tgccctggag gagacgatcc ccgacacgat
     2641 cagcgtcgag gatcaggcga tctccgctgc cctggccgag cgcatcgccg aggaggtggg
     2701 acggctcgac gagaccgacc gccgcatctt ccggttgtgc gcggtggagg gctacgccta
     2761 ccaggtcgcc gcggaggagc tcggcgtgag ccacgccgtc gtccgcaacc gtctgtcccg
     2821 ggttcgcacg cgcctgcgag gcgctgctca ggagatgagg gacgcatgag cgagaacgat
     2881 ggcgtggagc tgccgccgct gagcgacgag accatcgcac ggatcgagga ggcggtcttc
     2941 gacgacatcg ccgaagagcg gtcgctaccg cttccggcgc ggcagagcac agcgcgccgc
     3001 cggcggcgac gctgggcgct cggcgtgggt gtggctgcg
//
LOCUS       DATMSX010000172         8725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_59390,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000172 DATMSX010000000
VERSION     DATMSX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59390"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VN041_05260"
     CDS             complement(<1..973)
                     /locus_tag="VN041_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWU28487.1"
                     /translation="MRARLSHSAGRAAVLARRAAALFPVRVWRRFLRRNGFLLSAGMA
                     YQGLFALFALLFVGFASVGVWLGGSDSAVDALITVANSYLPGIIGEHGIAAPDQVREI
                     VQDAGTAFAATGLIAIGVAIWTAVGAVTFTRRAVRDVFGLPFDTRSFVVLKLRDALGA
                     FFFGAALIVGAVLSVFGVWALTQLLELLGWDLPNGTIRGTVSAGSVLVIVVIDTAAIA
                     LLVRFLTGTTIHWRTIWPGATAGGVTVAVLQLGIGLLFAHSPGNPLLATFAVMVALLL
                     WCRLVAAVILISATWIAVSASDRGEPLEQPDAAAAAHRFVVAREQEAR"
     gene            complement(991..1749)
                     /locus_tag="VN041_05265"
     CDS             complement(991..1749)
                     /locus_tag="VN041_05265"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWU28488.1"
                     /translation="MSNAVAEAPANDSGVQSFLVTFIVRRFDPEKDAEPHWVDYDVEL
                     YSTDRVLDALHKIKWEVDGSLAFRRSCAHGVCGSDAMRINGRNRLACKTLIKDLDISK
                     PIYVEAIKGLPLEKDLIVDMEPFFASYREVQPFLIANSAPPKDKERIQTIADREIFDD
                     TTKCILCAACTSSCPVFWTDGQYFGPAAIVNAHRFIFDSRDDAADVRLDILNDKEGVW
                     RCRTTFNCTEACPRGIEVTKAIAEVKQAVLRGGR"
     gene            complement(1752..3578)
                     /gene="sdhA"
                     /locus_tag="VN041_05270"
     CDS             complement(1752..3578)
                     /gene="sdhA"
                     /locus_tag="VN041_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWU28489.1"
                     /translation="MTTQTSESVVRDGIHYHEFDIVIVGAGGAGMRAAIEAGPGARTA
                     VISKLYPTRSHTGAAQGGMAAALANVEEDNWEWHTYDTVKGGDYLVDQDAAEILAKEA
                     IDAVIDLENMGLPFNRTPEGKIDQRRFGGHTSEHGKAAVRRACYAADRTGHMILQTLF
                     QNCVKLGINFFNEFYVLDLITVKDDAGATQVAGVVAYDLATGELHVFHSKAIIFATGG
                     FGKIFKTTSNAHTLTGDGVGIVWRKGLPLEDLEFFQFHPTGLAGLGILLTEGARGEGA
                     ILRNASGERFMERYAPTIKDLAPRDIVARSMVQEVLEGRGAGPHKDYVLLDCTHLGAE
                     VLETKLPDITEFARTYLGVDPVVEPVPVMPTAHYAMGGIPTNNNAEVLADNTTTVPGL
                     YAAGECACVSVHGSNRLGTNSLLDINVFGKRAGRNAVEYVKTAEFVPLPENPAGFVKD
                     MVDGLRNNQGTERIAVLRKKLQDEMDKGAQVFRTHESLEHVLGVIKELRERYKNVHVD
                     DKGRRFNTDLLEAVELGFLLDIAEVVVYAAQNREESRGGHMRDDFPKRDDENYMKHTM
                     AYLTGDPHSSTPADHIRLDWKPVVFTKNEKGELNYPPMERKY"
     gene            complement(3600..4052)
                     /locus_tag="VN041_05275"
     CDS             complement(3600..4052)
                     /locus_tag="VN041_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HWU28490.1"
                     /translation="MATQTVASAPVAARRQRKMNLEKVGWLFMRGSGVLLIVLIFGHL
                     FVNLMVGGGIHAIDFAFVAGKYATPFWQWWDVLMLWLALIHGSNGMRTIVNDYVTHAG
                     ARKALVWALGLAAALLIILGTLVVFTFDPCNGVLENGAMWETCQALGK"
     gene            complement(4052..4477)
                     /gene="sdhC"
                     /locus_tag="VN041_05280"
     CDS             complement(4052..4477)
                     /gene="sdhC"
                     /locus_tag="VN041_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWU28491.1"
                     /translation="MSTSTRLTPSISETTSKSPRGTLYRGREGMWSWVLHRITGVAIF
                     FFLLVHVLDTALIRVSPEAYNAVIGTYKIPIMTFGEIVLVAGIVFHAMNGLRIILVDF
                     WSKGAKYQRQMFWIVIGVWAVLMAGFASRHLMLAFGGAH"
     gene            4633..5745
                     /locus_tag="VN041_05285"
     CDS             4633..5745
                     /locus_tag="VN041_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HWU28492.1"
                     /translation="MGRIDDFHAVIPAGGIGSRLWPLSRADAPKFLHDLTGSGHSLLR
                     DTWDRLEPLAGPDRIAVVTGRAHRAAVEEQLPGIPDANVLLESEPRESAAAIGLAAAV
                     LHRRNPDVIIGSFSADHVIRGTQVFEWAVQQAVQVARQDYIVTIGIPPTEPSVGFGYI
                     KRAEEIVVDGAPEATLVERFVEKPDLETAKEYVADRDYLWNAGMFISKASVLLEELAV
                     NEPELHAGLLELAEAWDDRDRRGPAVDRIWPRLKKIAIDYAVAEPAAARGRLAVIPGH
                     FDWDDVGDFASLTKLITHGRKNDLAVLGPKARVLSDAASGILVSQTSRVISLVGVKDI
                     VVVDTPDALLVTTVEHAQRVKGVVESLKLTGRGDVL"
     gene            5905..7020
                     /locus_tag="VN041_05290"
     CDS             5905..7020
                     /locus_tag="VN041_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU28493.1"
                     /translation="MPISTTKKMLGAALATGVILALAGCGQAPTDTKPGDKPAASNFK
                     PCIISDDGGWNDRSFNQSAKEGLDKIVKELGVKSVELQSDSADQYGPNLEQAISQGCN
                     YIVTVGFKLSADTIKSAKANPKVNYAIIDDLADGDSDGKPDAPNIKPLLFDTVQAAYL
                     GGYAAASWSHEAGVDKVGTFGGLQIPPVTIFMDGFVQGVAKYNEDKGTDVKSVGWDAD
                     TQKGQMTGGFAANDTAKQTAQSILDQGVDVLLPVGGPIYQSAAAAITAQNKGTLLMGV
                     DSDLAVSDSSVADITLVSIQKKIGEAVYSAVKDASSGKVDTTPYIGTLENGGVGLSSF
                     HDFESKLPAGLTDELKALQEKIISGDIKVTSVSSPKA"
     gene            7103..8629
                     /locus_tag="VN041_05295"
     CDS             7103..8629
                     /locus_tag="VN041_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28494.1"
                     /translation="MKLELRGITKRFGSLVANDHIDLVVQPGEIHALLGENGAGKSTL
                     MNVLYGLYQPDEGEILLDDAVQHFAGPGDAMAAGIGMVHQHFMLIPVFTVAENVMLGH
                     ERTRRAGGLDTGILDIAEARQHVRDVAARFGFDIDPDAVVEDLPVGVQQRVEIIKALS
                     RNAKVLVFDEPTAVLTPQETDELMGIMRQLRDEGTAIVFITHKLREVREVADRITIVR
                     LGKIVGDALPTASNTELASMMVGRAVELTVHKNPPQVKESGGLEVRDLTVALPSGQLA
                     VDGVSFDVRPGEVLAIAGVQGNGQTELVEALVGLQTKGVTGSATLNGSELLGSSVRKV
                     LDEGVGFVPEDRTVDGLVGSFSVAENLMLDRSSDGAFVTGGTISRSRLDEFARDRITE
                     YDIRTQGPQTPAGALSGGNQQKVVIARELSRELTLLIASQPTRGVDVGSIEFIHKRIV
                     ETRDSGVPVVVVSTELDEVAALADKIAVMYRGRIMDIVPATTPRDVLGLLMAGEKDAA
                     "
BASE COUNT         1472 a         2915 c         2979 g         1359 t
ORIGIN      
        1 gtcgcgcctc ctgctcgcgg gccacgacga accgatgcgc ggccgccgca gcatccggct
       61 gctcgagcgg ttcgccgcga tcggaagcgc tcaccgcgat ccaggtcgcg gagatcagga
      121 tgaccgccgc gaccagccgg caccacagca gcagcgcgac catcaccgcg aacgtcgcca
      181 gcagcggatt gccgggggag tgggcgaaga gcaggccgat gccgagctgc agcaccgcca
      241 ccgtcacccc tcctgccgtc gcccccggcc agatcgtgcg ccagtggatg gtggtgccgg
      301 tcaggaagcg gacgagcagg gcgatggccg cggtgtcgat cacgacgatg acgagcaccg
      361 atcccgccga caccgtgccc cggatcgtcc cgttcggaag gtcccagccg agcagctcga
      421 gcagctgggt gagagcccac acgccgaaga cgctgagcac ggcgcccacg atcagggccg
      481 caccgaagaa gaatgcgccg agcgcatcgc gcagcttgag caccacgaag ctgcgggtgt
      541 cgaagggcag cccgaacacg tcgcgaacgg cccggcgggt gaacgtgacg gcgccgacag
      601 cggtccagat cgccacgccg atcgcgatca gacccgtggc cgcgaacgcc gtgcccgcat
      661 cctggacgat ctcgcgcacc tggtcgggcg cggcgatgcc gtgctcgccg atgatgccgg
      721 gcagatagct gttcgcgacg gtgatcagag cgtccacggc cgaatcgctg cctcccagcc
      781 agacgcccac ggaggcgaag cccacgaaga ggagtgcgaa cagcgcgaac agcccctggt
      841 acgccatgcc cgccgagagc aggaagccgt tccgtcgcag gaagcgccgc cagacgcgca
      901 ccgggaacag cgcggcggct ctgcgggcga gaacagcagc gcgcccggct gagtgactca
      961 gtcgggcgcg catggctgtg ctctggtcta tcagcggccg ccgcgcagga cggcctgctt
     1021 gacctcggcg atggccttgg tgacctcgat gccgcggggg cacgcctcgg tgcagttgaa
     1081 ggtcgtgcgg cagcgccaca cgccctcctt gtcgttgagg atgtccaggc gcacgtcggc
     1141 cgcgtcatcg cgcgagtcga agatgaagcg gtgcgcgttc acgatcgcgg ccggaccgaa
     1201 gtactgcccg tcggtccaga acaccgggca cgacgacgtg cacgctgcgc acaggatgca
     1261 cttggtggtg tcgtcgaaga tctcgcggtc ggcgatcgtc tggatgcgct ccttgtcctt
     1321 cgggggagcg gagttggcga tcaggaacgg ctgcacctcg cggtaggagg cgaagaacgg
     1381 ctccatgtcg acgatgaggt ccttctccag cggcaggccc ttgatggcct cgacgtagat
     1441 cggcttcgag atgtcgaggt ccttgatcag cgtcttgcag gccaggcggt tgcggccgtt
     1501 gatgcgcatg gcatccgatc cgcacacgcc gtgcgcgcac gagcggcgga aggccagtga
     1561 gccgtccacc tcccacttga tcttgtgcag ggcgtccagc acgcggtcgg tcgagtacag
     1621 ctcgacgtcg tagtccaccc agtgcggctc ggcgtccttc tcggggtcga agcggcgcac
     1681 gatgaacgtg acgaggaagg actgcacacc cgaatcgttt gcaggagcct ctgcgacggc
     1741 gttcgacatg ctcagtactt cctctccatc ggcgggtagt tcaactcgcc cttctcgttc
     1801 ttggtgaaga ccacgggctt ccagtccagc cggatgtggt cggccggcgt cgacgagtgg
     1861 gggtcgccgg tcaggtacgc catggtgtgc ttcatgtagt tctcgtcgtc gcgcttcggg
     1921 aagtcgtcgc gcatgtgacc gccgcggctc tcctcgcggt tctgcgcggc gtagacgacg
     1981 acctcggcga tgtcgagcag gaagcccagc tcgaccgcct cgagcaggtc ggtgttgaac
     2041 cggcggccct tgtcgtcgac gtgcacgttc ttgtagcgct cgcgcagctc cttgatcacg
     2101 cccaggacgt gctcgagcga ctcgtgcgtg cggaacacct gcgcgccctt gtccatctcg
     2161 tcctgcagct tcttgcgcag cacggcgatg cgctcggtgc cctggttgtt gcgcaggccg
     2221 tcgaccatgt ccttgacgaa gccggcgggg ttctccggca gcggcacgaa ctcggcggtc
     2281 ttcacgtact cgacggcgtt gcgtcctgcg cgcttgccga acacgttgat gtccagcagc
     2341 gagttggtgc cgaggcggtt ggagccgtgc accgagacgc acgcgcactc gccggcggcg
     2401 tacaggccgg ggacggtggt cgtgttgtcc gcgaggacct cggcgttgtt gttggtcggg
     2461 atgccgccca tcgcgtagtg cgcggtcggc atgacgggca ccggctcgac gaccgggtcg
     2521 acgcccaggt aggtgcgggc gaactcggtg atgtccggga gcttggtctc cagcacctcg
     2581 gcgcccaggt gggtgcagtc cagcagcacg tagtccttgt ggggtccggc accgcggccc
     2641 tcgagcacct cctgcaccat gctgcgcgcg acgatgtcgc ggggggcgag gtccttgatg
     2701 gtgggggcgt agcgctccat gaagcgctca cccgaggcgt tgcgcaggat cgcgccctcg
     2761 ccgcgggctc cctcggtcag caggatgccg agaccggcga gaccggtggg atggaactgg
     2821 aagaactcca ggtcctccag cggcaggccc ttgcgccaca cgatgccgac gccgtcgccg
     2881 gtgagggtgt gcgcgttgga ggtggtcttg aagatcttgc cgaagccacc ggtcgcgaag
     2941 atgatcgcct tcgagtggaa gacgtgcagc tcgccggtgg ccaggtcgta tgccaccacg
     3001 ccggcgacct gggtggcgcc cgcgtcgtcc ttgaccgtga tcaggtcgag cacgtagaac
     3061 tcgttgaaga agttgatgcc gagcttgacg cagttctgga acagcgtctg caggatcatg
     3121 tgaccggtgc ggtcggccgc gtagcaggcg cggcgcacgg ccgccttgcc gtgctcggag
     3181 gtgtgaccgc cgaagcggcg ctggtcgatc ttgccctccg gcgtgcggtt gaacggcaga
     3241 cccatgttct ccaggtcgat gaccgcgtcg atggcctcct tggccaggat ctcagcggca
     3301 tcctggtcga cgaggtagtc gccgcccttg accgtgtcgt aggtgtgcca ctcccagttg
     3361 tcctcctcga cgttggcgag tgccgcagcc atgccgccct gcgcagcacc cgtgtgcgag
     3421 cgcgtggggt acagcttgga gatcaccgcg gtgcgggcgc cgggcccggc ctcgatcgcc
     3481 gctcgcatgc ccgcgccgcc ggcgccgacg atgacgatgt cgaactcgtg gtagtggatg
     3541 ccatcgcgga cgacggactc ggacgtctga gtagtcactg tgtgttctgc cttctcgtct
     3601 tacttgccca gggcctggca ggtctcccac atggcgccgt tctcgagcac gccgttgcag
     3661 gggtcgaacg tgaacaccac gagggtgccc aggatgatca ggagcgccgc ggccaggccg
     3721 agggcccaca ccagcgcctt gcgtgcgccc gcgtgggtga cgtagtcgtt cacgatcgtg
     3781 cgcatgccgt tggagccgtg gatcagtgcg agccagagca tcagcacgtc ccaccactgc
     3841 cagaagggag tggcgtactt gccggcgacg aaggcgaagt cgatggcgtg gatgccgccg
     3901 ccgaccatca ggttcacgaa gaggtgaccg aagatcagca cgatcaggag cacaccggac
     3961 ccgcgcatga acagccagcc gaccttctcc aggttcatct tgcgctggcg gcgtgcggcg
     4021 acgggggcgg aagcgacagt ctgcgtggcc atcagtgagc ccctccgaac gcgagcatca
     4081 ggtgacgaga ggcgaagccg gccatgagga cggcccacac gccgatgacg atccagaaca
     4141 tctggcgctg gtacttcgcg cccttggacc agaagtccac caggatgatg cggaggccgt
     4201 tcatcgcgtg gaacacgatg cccgcgacca gcacgatctc accgaaggtc atgatcggga
     4261 tcttgtaggt gccgatgacc gcgttgtacg cctcgggcga gacgcggatc agcgccgtgt
     4321 ccagcacgtg caccagcagg aagaagaaga tggcgactcc ggtgatgcgg tgcagcaccc
     4381 aggaccacat tccctcgcga ccccggtaca gggttccgcg aggagacttc gaggtggttt
     4441 ccgatatcga cggtgtcaag cgtgtgctcg tggacacgtc gtcctccctg ctcgatcgag
     4501 acgtcgggtc cgtgcatccg ggcacgcccg acctcgacca tcctattcct gtcagaacgc
     4561 tgggtgcgac gaaggggacc cttagcgcgt cctccgacga ggatcacagc gggccccgat
     4621 aggttggtgc gcatggggcg aatcgacgac ttccacgcag tgatcccggc aggcggcatc
     4681 ggcagcaggc tgtggccgct gtccagggcc gacgccccga agttcctgca cgacctgacc
     4741 ggatccgggc actcgctgct gcgcgacacc tgggatcggc tggagcctct cgcgggtccc
     4801 gaccggatcg ccgtggtcac gggtcgtgcg caccgtgcgg cggtggagga gcagcttccg
     4861 ggcatcccgg acgccaacgt gctgctcgag tccgagccac gcgagtccgc ggccgcgatc
     4921 ggcctggccg cggcggtgct gcaccgccgg aacccggatg tgatcatcgg ctcgttcagc
     4981 gccgaccacg tcatccgcgg cacccaggtg ttcgagtggg cggtgcagca ggccgtgcag
     5041 gtggcgcggc aggactacat cgtcacgatc ggcatcccgc ccaccgagcc ctcggtcggc
     5101 ttcgggtaca tcaagcgcgc cgaggagatc gtcgtcgacg gcgccccgga ggccactctc
     5161 gtcgagcggt tcgtcgagaa gcccgacctc gaaaccgcga aggagtacgt cgcggatcgc
     5221 gactacctgt ggaacgccgg catgttcatc tccaaggcga gtgtcctgct ggaggaactc
     5281 gccgtgaacg agcccgagct gcacgccgga ctcctcgagc tcgccgaggc gtgggacgac
     5341 cgcgaccgca ggggacccgc cgtggaccgc atctggccgc ggctgaagaa gatcgcgatc
     5401 gactacgcgg tcgccgagcc cgccgccgcg cgcggccggc tcgccgtgat cccgggccat
     5461 ttcgactggg acgacgtggg cgacttcgcc tcgctgacca agctgatcac gcacggacgc
     5521 aagaacgacc tggcggtgct gggtccgaag gcgagggtgc tctcggacgc ggccagcggc
     5581 atcctggtca gccagacctc gcgggtgatc agcctggtcg gggtcaagga catcgtggtg
     5641 gtggacactc cggatgccct cctggtcacg accgtcgagc acgcgcagcg cgtgaagggc
     5701 gtcgtggagt cgctcaagct caccggtcgc ggcgacgtgc tctgacccct ccgtggctgt
     5761 ccgccaagcg gatcgcggag ttcgcattgc ggatttgtaa cctttcgcca gcacatccct
     5821 ggcgagtggt gcgcagaagc gtaaccgtgg gtaatttaga tcgatccgcg atgtccgcgg
     5881 gacagatctc aggggaggca aagagtgccc atctctacga ccaagaagat gctcggggcg
     5941 gccctcgcca ccggcgtcat cctcgctctc gccggctgtg gccaggcgcc gactgacacg
     6001 aagcccgggg acaagcccgc cgccagcaac ttcaagccct gcatcatctc ggatgacggc
     6061 ggctggaacg accgctcgtt caaccagtcg gccaaggagg gtctggacaa gatcgtcaag
     6121 gagctcggcg tcaagtccgt cgagctgcag tccgactcgg ccgaccagta cggtccgaac
     6181 ctcgagcagg cgatcagcca gggctgcaac tacatcgtca cggtcggctt caagctgtcc
     6241 gccgacacca tcaagtcggc caaggccaac ccgaaggtca actacgcgat catcgacgac
     6301 ctcgccgacg gcgacagcga cggcaagccg gacgcgccga acatcaagcc cctgctgttc
     6361 gacacggtgc aggccgcgta cctcggcgga tacgccgcgg catcctggtc gcacgaggcc
     6421 ggcgtcgaca aggtcggcac cttcggcggt ctgcagatcc cgccggtgac gatcttcatg
     6481 gacggcttcg tccagggcgt cgccaagtac aacgaggaca agggcaccga cgtcaagtcg
     6541 gtcggctggg acgccgacac ccagaagggt cagatgacgg gcggcttcgc ggccaacgac
     6601 accgccaagc agaccgcgca gtcgatcctc gaccagggcg tcgacgtgct gctgccggtc
     6661 ggcggcccga tctaccagtc ggctgccgct gccatcaccg cgcagaacaa gggcacgctg
     6721 ctcatgggcg tcgactccga cctggccgtc tccgactcct cggtcgcaga catcacgctc
     6781 gtctcgatcc agaagaagat cggcgaggcc gtgtactcgg ccgtcaagga cgcttcctcc
     6841 ggcaaggtcg acaccacgcc ctacatcggc acgctggaga acggcggcgt cggcctgtcg
     6901 tccttccacg acttcgagtc gaagctgccg gcaggcctca ccgatgagct gaaggcgctg
     6961 caggagaaga tcatctccgg cgacatcaag gtcacctcgg tctcgtcgcc gaaggcctga
     7021 cgaaaccaac cagatgcggc cggacggctg gaccgtccgg ccgcatctgt catgccacgc
     7081 aagtcctgag tagggtgcag atatgaagct cgagctccgc ggaatcacca agcgattcgg
     7141 cagtctcgtc gccaatgacc acatcgatct ggtggtccag cccggggaga tccacgctct
     7201 cctcggcgag aacggcgcag gcaagtccac gctcatgaac gtcctgtacg ggctgtacca
     7261 gcccgatgag ggtgagatcc tgctggacga cgccgtccag cacttcgccg gccctggaga
     7321 cgccatggcg gccggcatcg gcatggtgca ccagcacttc atgctgatcc ccgtgttcac
     7381 ggtcgccgag aacgtgatgc tcggtcatga gcgcaccagg cgcgccggtg gcctcgacac
     7441 cggcatcctc gacatcgccg aggccaggca gcacgtccgc gacgtcgcgg cccgcttcgg
     7501 cttcgacatc gatccggatg ccgtggtcga agacctcccc gtgggcgtgc agcagcgggt
     7561 cgagatcatc aaggcgctct cccgcaacgc caaggtgctc gtcttcgacg agcccactgc
     7621 ggtgctgacc ccgcaggaga cggatgagct gatgggcatc atgcgtcagc tgcgcgatga
     7681 gggcacggcg atcgtcttca tcacccacaa gctgcgcgag gtgcgcgaag tggccgaccg
     7741 gatcaccatc gtgcgtctgg gcaagatcgt cggcgacgcg ctgccgaccg cgtccaacac
     7801 cgagctcgcc tcgatgatgg tcggccgcgc cgtggagctg accgtgcaca agaacccgcc
     7861 gcaggtgaag gaatccggcg ggctcgaggt ccgcgacctc accgtcgcgc tgccctccgg
     7921 ccagctggcc gtggacggcg tgagcttcga cgtgcgcccg ggcgaggtgc tcgccatcgc
     7981 cggcgtgcag ggcaacggtc agaccgagct cgtcgaggcg ctcgtgggcc tgcagaccaa
     8041 gggcgtcacc gggtcggcca cgctgaacgg cagcgagctg ctcggcagct cggtgcgcaa
     8101 ggtgctcgac gagggggtcg ggttcgtccc cgaggaccgc acggtcgacg gcctggtcgg
     8161 ctccttctcg gtcgctgaga acctgatgct cgatcgctcc tcggacggcg cgttcgtgac
     8221 cggcggcacc atcagccgca gcaggctgga cgagttcgcc cgcgaccgca tcaccgagta
     8281 cgacatccgc acccagggtc ctcagacccc ggccggcgca ctctccggcg gcaaccagca
     8341 gaaggtggtc atcgcgcgcg agctgagccg cgagctgacg ctgctgatcg catcccagcc
     8401 gactcgagga gtcgacgtcg gctccatcga attcatccac aagcggatcg tcgagacccg
     8461 cgactccggc gtccccgtgg tcgtcgtctc gaccgagctc gacgaggtgg ccgccctggc
     8521 ggacaagatc gcggtgatgt accgcggccg gatcatggac atcgtcccgg ccacgacccc
     8581 gcgcgacgtg ctcggcctgc tgatggccgg agagaaggac gcagcatgag cgaccagacc
     8641 ccccgtcccg accgcgtgcc ctcggggccg acggcggcac ccgaggccgt cgcggcggag
     8701 aagggcatca gctcgggtgc cgcgg
//
LOCUS       DATMSX010000173         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_106982,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000173 DATMSX010000000
VERSION     DATMSX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106982"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VN041_05300"
     CDS             <1..1128
                     /locus_tag="VN041_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28495.1"
                     /translation="EASADEVDEAEDADHGTDADETDADETDGDETDGDEADAAGSEA
                     NADDANADDAGDAHADAAEDTADGDGDLSRDADDSADDTHFDADGSANAEDPDNGRSG
                     DETDGEVETDDDSQNDPSAHDDSAAQGDPDAHGDDASEHAEHAEHAASDADGDASAPD
                     DPATDEAQTEDDDASTSLHDAVAGLWAPRVADTADTGPADDEDEDEDETATRDEDAPE
                     QPAAPVDPADFFTDPDERPGVLPNATGAISISPLTDRTTFTELITGIPASASLGDTEA
                     PAAATEAIPVFTPPLSHDEDDDEGVVDDIPEDDPRLAAMRSSLSSAASRFFDGPAPDY
                     PYGDVPKPDAADEQESADAEQSDDHDGDEGDENPEGDQHHG"
     gene            complement(1154..1600)
                     /locus_tag="VN041_05305"
     CDS             complement(1154..1600)
                     /locus_tag="VN041_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28496.1"
                     /translation="MVTLLLDQTRLEVVLSPIERASTFHRENVRVEREHITRVQLTDD
                     AWTWLRGVPGPGTYIPSILAAGTWKGAGTTDFVLIRKHRPSVVIDLEGDEQYQRLILT
                     TKHGLALTQALRLEVSAEPADVVEIATGSVPVTKPRQRPVIRPRPA"
     gene            complement(1708..1965)
                     /locus_tag="VN041_05310"
                     /pseudo
     CDS             complement(1708..1965)
                     /locus_tag="VN041_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
BASE COUNT          356 a          754 c          725 g          226 t
ORIGIN      
        1 gaggcgagcg ccgatgaggt ggacgaggcc gaggacgccg accacgggac cgacgccgac
       61 gagacggacg ccgacgagac cgacggcgac gagaccgacg gcgacgaggc cgacgccgct
      121 ggcagtgagg cgaacgccga cgacgcgaac gctgacgacg cgggcgacgc ccacgcggac
      181 gccgccgagg acaccgcgga cggcgatggg gacctgtcgc gcgacgccga tgactcggcg
      241 gacgacaccc acttcgacgc cgacggatcc gcgaacgccg aggacccgga caacggacgc
      301 tcgggcgacg agaccgacgg cgaggtcgag accgacgacg acagccagaa cgaccccagc
      361 gcccacgacg actccgccgc tcagggcgac cccgacgcgc acggcgacga cgcatccgag
      421 cacgccgaac acgccgaaca cgcggcatcc gacgccgacg gcgacgcctc cgccccggat
      481 gaccccgcga cggacgaggc gcagaccgag gacgatgacg cctccacgtc gttgcacgac
      541 gcggtggcgg gactctgggc accgcgcgtc gcggacaccg ccgacaccgg cccggcagac
      601 gacgaggacg aggacgagga cgagactgca acccgcgacg aggatgctcc cgagcagccc
      661 gccgcaccgg tcgacccggc cgacttcttc accgaccccg acgagcgtcc cggcgtcctt
      721 ccgaacgcca ccggcgccat ctcgatctcg ccgctgaccg accggacgac gttcacggag
      781 ctgatcaccg gcatcccggc atccgcgtct ctcggcgaca ccgaggctcc cgcggcggcc
      841 accgaggcga tccccgtctt cacgccgccc ctgtcgcacg acgaggacga tgacgagggc
      901 gtcgtggacg acattcccga ggacgacccg cgcctggccg cgatgcgcag ctcgctctcc
      961 agcgccgcca gccgcttctt cgatggcccc gcgccggact acccctacgg cgatgtcccg
     1021 aagccggatg ccgccgatga gcaggagtca gcggacgccg agcagtcgga cgaccacgac
     1081 ggcgacgagg gcgacgagaa ccccgagggc gaccagcacc acggctgatc gccgcggacc
     1141 gcggaaagtc gggtcaggcc ggccggggcc ggatgaccgg acgctgacgc ggcttcgtca
     1201 cgggcacgga tcccgtggcg atctcgacga cgtcggcggg ctcggcgctc acctcgaggc
     1261 gcagcgcctg ggtgagcgcg agtccgtgct tcgtggtgag gatcagccgc tggtactgct
     1321 cgtcaccctc gaggtcgatg acgacgctgg gccggtgctt gcggatcagc acgaaatccg
     1381 tcgtccccgc acccttccag gtgcctgcgg ccaggatgct ggggatgtag gttccgggtc
     1441 cggggacgcc acgcagccag gtccacgcgt cgtcggtgag ctgcacgcgg gtgatgtgct
     1501 cgcgctcgac gcgcacgttc tcgcggtgga aggtcgatgc gcgctcgatc ggcgacagga
     1561 ccacctcgag tcgcgtctga tccagcagga gcgtcaccat caccccagtc tgccagcgcc
     1621 gccctgcaag atgcctgcga cttgctcaca aggcggcaac gatccgttgt gcgatcgaca
     1681 aacgggagag cgaccggtcg cgcggagtca ggcggtggcg gatgccgcgg ccgccgcggc
     1741 cgcgggcagt gcggcctcga tcgcggcgag ctccacctcg tcgtgtgcgg ccgtgaggaa
     1801 ccacgcctcg aacacgctcg ggggcagcgc gatcccctgc tcgcgcatgg cgtggaagaa
     1861 cggcgcgtac cggaacgact gctgtgcctg cgcctcggcg taggtcttcg gcgcctcggc
     1921 cgagaaggcc acgccgaaca ggctgccggc gcgcggcacg gcgtgcacgg ccgagctcct
     1981 gcacactcag gtgcagcgct tcgagacgct gctgaacgac ctgctcgaga tcagccgcta
     2041 cgacgccggt tccgtgcagc t
//
LOCUS       DATMSX010000174         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_144308,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000174 DATMSX010000000
VERSION     DATMSX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144308"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VN041_05315"
     CDS             <1..318
                     /locus_tag="VN041_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28497.1"
                     /translation="LDGVSLAAVLREPAQTFERPLYWRMNHRQQRALRKGPWKYLRVD
                     DHEYLFDLNRDERERANLAKRHPDIFEALRAQWLAWNETVPPIPEDAAVSLGYSAADM
                     PQR"
     gene            597..1277
                     /locus_tag="VN041_05320"
     CDS             597..1277
                     /locus_tag="VN041_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWU28498.1"
                     /translation="MSALDTALHGLRALIADGTLRPGDRLPSEGELCEQLGVSRGSLR
                     EAIRMLAALGVLETRHGSGSYVGDLRAADLIRSLSLTVGLLPMEGVLELTELRRALEP
                     HVTALAASRIDRDTLEQLERLLDENEATTDFDEQSRIDHEFHMTIARVAGQDALTSLI
                     DVLRSRSRSYRMANSEDAAELKLHSDAGHRAILRGLTDGDPVAASSAASAHVAYTEYW
                     VKKNSALS"
     gene            complement(1317..2705)
                     /locus_tag="VN041_05325"
     CDS             complement(1317..2705)
                     /locus_tag="VN041_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWU28499.1"
                     /translation="MSDDRDARVISALIAAVPDGAVITNPDSLEAYRRDRAEDPDAGT
                     PLAVVRATTTEDVQAVVRIAAQEGVGVIPRGAGSGLSGGSSAVDGAIVISLERMREIR
                     IDPALQMAVVQPGAFNAEVKAAASEHGLWYPPDPSSQEFCSIGGNIATNAGGLCCVKY
                     GVTTDYVLGLTVVLADGRAITLGGPRLKDVAGLSLTKLFVGSEGTLGIVTEAILRLVP
                     AQRTPTTLVAMFPTVEGAIRAVVDIKAAMRPSMLEFMDRVTINAVEDMMRMDLDRESA
                     AMLIVQSDESVEVAAAQIEAIERTCGLHGASEVYATSDPGESEALIEARRQAIPAVEK
                     RGALLLEDVGVPLPHLGDLIDGMRAIAEARGVEIAVVAHAGDGNTHPLIVLDPTDAEQ
                     RANADLAFGEVMALAIELGGTITGEHGVGRLKQPWLADYLGDDVLELNLRIKKALDPQ
                     NILNPGAMFGPL"
     gene            complement(2784..3293)
                     /locus_tag="VN041_05330"
     CDS             complement(2784..3293)
                     /locus_tag="VN041_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWU28500.1"
                     /translation="MTTLILTVAGADRPGLVSAVADLTGAYGCNWENSSLAELAGTFA
                     GVIQLTVADDRVDELRDALRGLDGMLAVTVSTGSDVTTASEERHVSVTVLGDDRPGIV
                     REVSGVLSAHALSIRSMTTETRDAAMAGGRLFESAVTASAPASADLDALRADIERLTH
                     DLQVDISIL"
     gene            complement(3326..4378)
                     /locus_tag="VN041_05335"
     CDS             complement(3326..4378)
                     /locus_tag="VN041_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HWU28501.1"
                     /translation="MSEWVSSAIRVLEADANRSADTHLHLFPLPTDWGIDLYLKDESV
                     HPTGSLKHRLARSLILYGLVNGLIDEGTTLVESSSGSTAVSEAYFARMLGLDFITVVP
                     RSTSQEKIDLIEFYGGNCHLVESADQISPEARRLAAGCNGHYLDQFTYAERATDWRGN
                     NNIAESVFSQLAEERHPIPRWIVVGAGTGGTSATFGRYVRYRRHPTQVAVVDPEGSAF
                     YAGWKGDADAAGSPSRIEGIGRPRVEASFVPSVIDEMIQVPDAASVAAIRLLRERTLH
                     WAGGSTGTNLYGAFELIGRMRDAGETGSVVTLICDGGARYAETYYSDDWVAAQGWDLA
                     PHRSRMEAFLAGDAWS"
     gene            complement(4410..>5069)
                     /locus_tag="VN041_05340"
     CDS             complement(4410..>5069)
                     /locus_tag="VN041_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28502.1"
                     /translation="ATQPGNPAPQSYGLPPTPVWGGQPSPVTPASSGPGHQPEQTSQR
                     WLTAVIVGGIVVGIAVIFVLIRMLLVGPAYLFDFSSGPAPASTSDSDTTATGSGEDPA
                     TGEGSSTGSAATIGDELQAKIDEYRTARDDGSLWQKIPDNEFNRTAVTAFLYIVTDLK
                     AATIWGVTDHQAREYTAKVADVEAKLLAQQPLGEDIHIELETRTFDYDGSTGEGGYTD
                     N"
BASE COUNT          762 a         1737 c         1781 g          789 t
ORIGIN      
        1 ctggacggcg tgtcgctggc cgccgtgctg cgcgagcctg cgcagacctt cgagcgcccg
       61 ctgtactggc gcatgaacca ccgccagcag cgcgcgctgc gcaagggccc gtggaagtac
      121 ctgcgcgtgg acgatcacga gtacctcttc gacctgaacc gcgacgagcg cgaacgcgcc
      181 aacctggcga agcgccaccc cgacattttc gaggcgctgc gcgcgcaatg gctggcctgg
      241 aacgagaccg tgccgcccat tcccgaagac gcggccgtga gcctgggcta ctcggccgcc
      301 gacatgccgc agcgctgagc cggggcgcgg gccgtggttg aggtggacga cgctcggatc
      361 caggggccac gcatcacgcg cacgggcgaa ggagttcagc cggagtgggg cgggagcggg
      421 ggtgtccgtc attggtcctc atctgtcatt acagcaagtt gttgcacaac cttgccacaa
      481 tcgtcgcgag aatgccaggg aagtctgccc agaagtcgga agttgttata caaatatggg
      541 ggaggggcga aatccgtcag aattcaggaa tccggctcga ggggagaggg gcacgcatga
      601 gcgcgctcga cacggcactg cacggcctgc gtgcgctcat cgccgacggc acgctgcgcc
      661 ccggcgacag gctgcccagc gagggcgaac tgtgcgaaca gctgggagtc tcgcgcgggt
      721 cgctgcggga ggcgatcagg atgctcgccg ctctcggggt gctggagacc cggcacggct
      781 ccggcagcta cgtcggcgac ctccgcgccg ccgacctcat caggagcctc tcgctgaccg
      841 tcggactgct gcccatggaa ggcgtgttgg agctgaccga gctgcgcaga gcgctcgagc
      901 cccatgtcac ggccctcgcg gcatcccgga tcgaccggga caccctggaa cagctggagc
      961 gactcctcga tgagaacgag gcgacgacgg acttcgacga gcagtcgcgg atcgatcacg
     1021 agttccacat gacgatcgcg cgcgtggcgg ggcaggatgc gctcaccagc ctcatcgatg
     1081 tgctcagatc gcgttcccgg tcgtaccgca tggcgaactc cgaggacgcg gcggaactga
     1141 aacttcactc cgatgccggc catcgcgcga tcctgcgcgg gctgaccgac ggggatccgg
     1201 tcgccgcgtc gtccgcggcc tcggcgcacg tcgcctacac ggagtactgg gtcaagaaga
     1261 actcggcgct cagctgaccc gggcttccga ctcggaggcc cttctgccgg gcgtcgtcac
     1321 aggggcccga acatcgcgcc cgggttgagg atgttctgcg ggtcgagcgc cttcttgatg
     1381 cgcaggttca gctccagcac gtcgtcgccg aggtagtcgg ccagccaggg ctgcttgagc
     1441 cgcccgacgc cgtgctcgcc ggtgatcgtg ccgcccagct cgatcgcgag ggccatgacc
     1501 tcgccgaagg cgaggtcggc gttggcgcgc tgctcggcat ccgtcgggtc cagcacgatc
     1561 agcggatgcg tgttgccgtc tccggcatga gcgaccacgg cgatctcgac gccgcgcgcc
     1621 tcggcgatcg cgcgcatgcc gtcgatcaga tcgcccaggt gcggcagcgg cacgccgacg
     1681 tcctccagca gcagggcccc gcgcttctcc accgccggga tcgcctgccg ccgcgcctcg
     1741 atgagcgcct cgctctcgcc cggatcgctg gtcgcgtaca cctcggatgc cccgtgcagc
     1801 ccgcaggtcc gctcgatggc ctcgatctgc gcagccgcca cctcgacgga ctcgtccgac
     1861 tgcacgatga gcatcgccgc cgactcgcgg tcgaggtcca tccgcatcat gtcctcgacc
     1921 gcgttgatcg tgacgcggtc catgaactcc agcatcgacg ggcgcatggc ggccttgatg
     1981 tcgaccaccg ccctgatcgc gccctcgacc gtcgggaaca tcgcgacgag tgtggtcggc
     2041 gtgcgctgcg cagggacgag ccgcaggatc gcctccgtga cgatcccgag cgtgccctcg
     2101 ctgcccacga acagcttggt gagcgagagc ccggccacat ccttcagccg cggcccgccg
     2161 agcgtgatcg cccgtccgtc cgcgagcacg accgtcaggc ccagcacgta gtcggtggtg
     2221 acgccgtact tcacgcagca cagcccgccg gcgttggtgg cgatgttgcc gccgatcgag
     2281 cagaactcct gcgacgacgg gtcgggcggg taccagagcc cgtgctcgga tgccgccgcc
     2341 ttgacctcgg cgttgaaggc gcccggctgc accacggcca tctgcagggc ggggtcgatg
     2401 cggatctccc gcatcctctc cagcgagatc acgatggccc cgtccacggc ggacgatccg
     2461 cccgacagcc ccgagccggc tccgcgggga atcactccga ccccctcctg cgcggcgatg
     2521 cgcacgaccg cctgcacgtc ctcggtggtg gtcgcccgca ccaccgcgag cggagtcccg
     2581 gcatccggat cctccgcgcg gtcgcgccgg tacgcctcca gggagtcggg gttcgtgatg
     2641 acggcgccgt ccggcactgc ggcgatgagg gcggagatca cgcgcgcgtc gcggtcatcg
     2701 gacatgggca tcctcggcat tcccgccacc cacctcggcg gcgctcccac aactgtggca
     2761 catgcgggat gacggcagcc ttgtcacagg atgctgatgt cgacctgcag gtcgtgcgtg
     2821 agcctctcga tgtcggcgcg cagggcatcc agatcggcgg atgccggtgc cgacgccgtc
     2881 acggcggact cgaacagccg cccgccggcc atggcggcgt cgcgggtctc ggtcgtcatg
     2941 ctgcggatgc tgagcgcatg cgcgctgagc acgccggaca cctcgcggac gatgccgggg
     3001 cggtcatcgc ccagcaccgt gacggacacg tgccgctcct cggacgccgt ggtcacgtcc
     3061 gacccggtgc tgacggtcac ggccagcatc ccatcgaggc cgcgcagggc gtcgcgcagc
     3121 tcatcgacgc gatcgtccgc gacagtcagc tggatgacgc cggcgaaggt gccggcgagc
     3181 tcggcgaggc tgctgttctc ccagttgcac ccgtaggcgc ccgtgagatc ggcgacggcc
     3241 gagacgagtc cggggcgatc cgctcccgcg accgtgagga tgagcgtggt catgcgctca
     3301 ctgtagtcgc gaggccgtcg tcctgtcagg accaggcgtc tcccgccagg aaggcctcca
     3361 tgcgcgagcg gtgcggggcc aggtcccagc cctgcgcggc cacccagtca tccgagtagt
     3421 aggtctcggc atagcgggcg ccgccgtcgc agatcagcgt gacgacgctg ccggtctcgc
     3481 ccgcgtcgcg catccggccg atcagttcga aggcgccgta gaggttggtg ccggtcgagc
     3541 cgccggccca gtgcagggtg cgctcgcgca gcagccggat ggcggcgacc gatgcggcat
     3601 ccggcacctg gatcatctcg tcgatcacgc tcggcacgaa cgaggcctcc acgcgcgggc
     3661 ggccgatgcc ctcgatgcgg gacgggctgc ctgcggcatc cgcgtcgccc ttccagcccg
     3721 cgtagaacgc ggagccctcc gggtcgacca ctgccacttg cgtggggtgg cgacgatagc
     3781 gcacgtagcg accgaacgtg gcgctggtgc cgccggtgcc cgcaccgacc acgatccatc
     3841 gggggatcgg atgccgctcc tcggctagct ggctgaacac gctctcagcg atgttgttgt
     3901 tgccgcgcca gtcggtggcg cgctcggcgt aggtgaactg gtcgagatag tggccgttgc
     3961 agccggccgc cagccggcga gcctcggggg agatctgatc ggcgctctcg accaggtggc
     4021 agttgccgcc gtagaactcg atgaggtcga tcttctcctg gctggtggag cgcggcacga
     4081 cggtgatgaa gtcgaggccc agcatccgtg cgaaatacgc ctcgctcaca gcggtcgatc
     4141 cgctggagga ctccaccagt gtggtgccct cgtcgatcag cccgttgacc aggccgtaga
     4201 ggatcagcga gcgggcgagg cgatgcttga gcgagccggt gggatgcacc gactcgtcct
     4261 tcaggtacag gtcgatgccc cagtcggtgg gcagcgggaa caggtgcagg tgcgtgtcgg
     4321 cggagcggtt ggcatccgct tccaggaccc ggatggcgga actgacccat tcactcatgc
     4381 ttcgagcgta gcggggcgct ccgtccgggt cagttgtccg tgtagccgcc ctcgcccgtg
     4441 gatccgtcgt agtcgaacgt gcgcgtctcg agttctatgt ggatgtcctc gccgagcggc
     4501 tgctgggcga gcagcttcgc ctcgacatcg gcgaccttcg cggtgtactc ccgagcctgg
     4561 tggtcggtca cgccccagat cgtcgccgct ttcaggtcgg tgacgatgta caggaacgcg
     4621 gtcacggccg tgcggttgaa ctcgttgtcg gggatcttct gccagagcga cccgtcgtcg
     4681 cgcgccgtgc ggtactcatc gatcttcgcc tgaagctcgt cgccgatcgt ggcggctgaa
     4741 cccgtggagg agccttcgcc ggtcgccggg tcttctccgg agccggtggc ggtcgtgtcc
     4801 gaatcggacg tcgatgcggg tgccgggccc gagctgaagt cgaacaggta ggcggggccg
     4861 acgagcagca tccggatgag cacgaagatg accgcgatgc ccacgacgat cccgccgacg
     4921 atcacggccg tgagccagcg ctgagaggtc tgctcgggct gatggcccgg cccggaggat
     4981 gccggcgtca caggggaagg ctgcccgccc cagaccgggg tcggcggcag gccgtacgac
     5041 tgcggggcgg ggtttccggg ctgtgtggc
//
LOCUS       DATMSX010000175        13533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_87987,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000175 DATMSX010000000
VERSION     DATMSX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87987"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VN041_05345"
     CDS             complement(<1..485)
                     /locus_tag="VN041_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28503.1"
                     /translation="MRPSRRVPLLRGFVGATIATFVALASHVWVGGYMPGMLGIAVPW
                     LLSLMACTLLAGHRLSLLRLSASVIISQLLFHVLFVLGSITPQGGLGPHVHGVVPLSF
                     GGDAPLLLPQDAAMWFAHAVAAALTVAALHRGELIARALLTVARAVATWLSRAVPAVD
                     V"
     gene            complement(527..1810)
                     /locus_tag="VN041_05350"
     CDS             complement(527..1810)
                     /locus_tag="VN041_05350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWU28504.1"
                     /translation="MHLRSSRRLRAAVVGAVALALALTGCTGSSQPELTYTPPKQAAG
                     ALPDDVTAQLQTAVQQAMAATGSSGAVVGVWVPWSGQWVTGLGTQAAGDKTKVTKDMS
                     FRIAAVTRMMTCDVLYGMADAGDIKLDAKVPKYVSGVADLGDITLLDLCNGTSGVGSS
                     AETAKPYWLNTPDRQWAPLQLASFGLGQQRGPAHTAYRDSDANYLMLGLALERESGLT
                     ASQLIKKYVTAPLGLESTSLPSPAAAEPEPAPALKGAYLSPAEGGGYNCAAPVDITKS
                     SSSIGFTDSGAVSTIEDLGRYAQAEARQALRDKDAKPGRFEKPLPASADAPAWYQATG
                     GAYLVGSMVGQHGWVPGYATAAYSDPATGFTVAVTLNNSSAGGGVAAYLAWELAAIAS
                     KAPAAKGETAPDFGLPFTAEQYAQTIADGAICKAG"
     gene            complement(1873..3264)
                     /locus_tag="VN041_05355"
     CDS             complement(1873..3264)
                     /locus_tag="VN041_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU28505.1"
                     /translation="MGCVSVTLSDEQQALFRLIEDTDEHVFITGRAGTGKSTLLQHFA
                     WNTQKQIAICAPTGVAALNVEGQTIHSLFRLPIGLIAESEIEQSDATRRILNAIQTLV
                     IDEISMVNADLMDAIDRSLRQARGKRGIPFGGVQVVMFGDPYQLAPVPPRGDELRYIQ
                     DHYRSFWFFDAKVWTGGLAGDGGGMLDIGEHGAKLHVHELVQIHRQSDDGFKSMLNAV
                     RYGRVTKDIADVLNERGARTPPDPEPGEPPIITLATRNDIVNSINSRHLAALAGREQT
                     AHAEVSGDFGRGEGNYPADAELKLKVGAQVMFLRNDVGMQGEPPRWVNGSIGTVTRIL
                     GGTVRVDIDGEEFDVEPAVWERLRYAYNPGTKTLSREVVAEFTQFPLRLAWAVTIHKS
                     QGKTYDRAIVDLGSGAFAPGQTYVALSRLTSLDGLYLSRPLRPRDIQVDEDVRRFMRE
                     AWEARQAEQVAKA"
     gene            3326..4399
                     /locus_tag="VN041_05360"
     CDS             3326..4399
                     /locus_tag="VN041_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28506.1"
                     /translation="MMQGRGVSQRRSALRTDLTILGVVGVLLIAALGAGGMSLYQQVY
                     SPSAFVQRYLDLLASGRAADALQVPGVSVDLSIKKDTDIHATASEALLRTAALAQLTD
                     YTVTSETQKGGVYRVSTAYRAGGVRGTSTYRVVQDGWVGVVPNWRFERSPIAEVELTV
                     RGADVFKVNGFELDRRQVSDKGVGASPLDPLHLLVFTPGAYSVTVDTPIAATPGVRVL
                     ADKALAHTPVDVQAQPTAKFTELVQQRVGEFLTACATQQVLLPTACPFGLEVSNRLAD
                     GSLPKWSITQQPVVTVVPDGANWKIPPTDAVAHIVVSIKSVFDGSVREVSEDVPFQID
                     GTISVQPDGTASIRVGSPNIDAE"
     gene            complement(4421..6427)
                     /locus_tag="VN041_05365"
     CDS             complement(4421..6427)
                     /locus_tag="VN041_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWU28507.1"
                     /translation="MKSYRLVGLAILLAGAAFGLIAALVVGGGAVPPKLLDPGPIVMW
                     GLPAVKLVANLAGAAMLGSLLLALFGLRSGSRPFDIALDTASVGAAIFTVSSAIAAGM
                     TLLAAFNPKLSADRTFGEQLGTFLLTLPLGQAWLITVLMGAVITLLAFGWRSWTGTLM
                     TAGLAAASFLPLATQGHSGDVEGHNIAVNAILLHTLGAAAWVGGILLLIILRSAANRT
                     NGKAKKVDALDLPVLVERYSTIALIAFIVVAVSGIARTVVALGSWSELFSPYGLIVLG
                     KAILLLCLGLFGAWYRRRLIPRFSGADEKRSFWTLILGELLLMGLASGAAAALARTPP
                     PVGEQAPANPTAAQRLTDSPLPPELTLDRWFTSYDIDVLWAVVIGFAVFFYIAGVVRL
                     RRRGDAWPVHRTIFWLAGMLLLLWVTSGPLNAYQEYLFSVHMAGHMVLSMAIPLLLVS
                     GAPITLALRAIHKREDGTRGAREWIMWAVHSPFSRVITHPIVAAAIFIVSLWAFYFTD
                     LVRWSLYDHLGHEWMVIHFLISGYLFVMSLVGVDPVPYRLPHAGRLITLIAVMAMHAF
                     FGMALMMQNGLMVAEWFGSMGRTWGPTPMEDQYIGGGIAWSVGEIPTLILAITVAIQW
                     SRSDDKLQRRSDRAADRDGDAELEEYNARLSKLAEQDRRAASRS"
     gene            complement(6577..6864)
                     /locus_tag="VN041_05370"
     CDS             complement(6577..6864)
                     /locus_tag="VN041_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWU28508.1"
                     /translation="MADKSITKTELVASIASATGESQATVSRVLDSLFGTVSDAVAKG
                     SKVSIPGWISFEQVATAARTGRNPQTGAEIKIPAGKRVKVTAGSKLKAAVK"
     gene            complement(7233..7538)
                     /gene="rpsN"
                     /locus_tag="VN041_05375"
     CDS             complement(7233..7538)
                     /gene="rpsN"
                     /locus_tag="VN041_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HWU28509.1"
                     /translation="MAKKSKIAKNEQRKVIVARYAERRAELKKTLVDPNATDEAREAA
                     RIGLQKLPRNASPVRVRSRDAIDGRPRGVLTKFGISRVRFRDMAHRGELPGITKSSW"
     gene            complement(7542..7712)
                     /gene="rpmG"
                     /locus_tag="VN041_05380"
     CDS             complement(7542..7712)
                     /gene="rpmG"
                     /locus_tag="VN041_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWU28510.1"
                     /translation="MAKKAQDVRPIIKLRSTAGTGYTYVTKKNRRNTPDRLVLKKYDP
                     VVRKHVEFREER"
     gene            complement(7712..7948)
                     /gene="rpmB"
                     /locus_tag="VN041_05385"
     CDS             complement(7712..7948)
                     /gene="rpmB"
                     /locus_tag="VN041_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWU28511.1"
                     /translation="MAAVCQVTGAVPGFGHNVSHSHRRTKRRFDPNVQKKTYYVPSLG
                     RKITLNVSAKGIKVIDARGIEKVVADLKAKGVKL"
     gene            complement(8108..8992)
                     /locus_tag="VN041_05390"
     CDS             complement(8108..8992)
                     /locus_tag="VN041_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28512.1"
                     /translation="MSATSTASVVTGDARRTASAPRSPWPARLAIGAGVLLAVLLQLL
                     PFYITVTSAMKARSDLSSQWLPPFSGIHWQNFATAIEQGGILRAIGNSAIVTLGATVL
                     VCVLGALAAYPLARRPTIGNKLVLAGVVGLIMIPPLSILVPLYSMMSQWHAINTYWGT
                     ILVMTATQLPLAIFLYTAFMRGLPISIEEAASVDGANAVQVLFRVVVPMLKPVTATVV
                     ILASVNVWNDYALSVYLMRSPEMRTIAPATATFFSTTSSDVGAAAAASLLSALPVLIV
                     YLVLQKYFIKGMVAGSEK"
     gene            complement(8989..9918)
                     /locus_tag="VN041_05395"
     CDS             complement(8989..9918)
                     /locus_tag="VN041_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28513.1"
                     /translation="MTTTVRTVHRPKRGTEGLVPRFASRGLNLLYLPALAVLGVFMLW
                     PLLNGVLLSLTNWDGYSPDRSFVGPANYLRLFEDPNFRTALGNTFLYGIGSTVLQQVI
                     GLGLALALDRHVRGRGVARAIIYLPVLVSPIVMGTMYYLFLAYNGGGLNDLVIALGGE
                     RTAWLSDSGTAIALIVVVNSLQFVGISMVIYLAGLQSIPTMYYEAARLDGADAAQSFR
                     SITLPMLQPAFATSIVLNLIGGMKLFDVIQVLTAGGPGYSTNSVSTLIAKMYFGSQRA
                     GYASAMGVVLFLIIAVFTLVLNTALNRRRLEQL"
     gene            complement(9915..11177)
                     /locus_tag="VN041_05400"
     CDS             complement(9915..11177)
                     /locus_tag="VN041_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28514.1"
                     /translation="MKTSLNSKRLLAGAAVATTAALALSGCSGDSSGTGGDVKIEMQT
                     NFGATDPSLKVLKKITDAYEKDNPGVKIELVPSTDTYEDDIKVRLSSNDIPDIWATHG
                     WSLLRYSKFLEPLDDQPWAKDFNKALAPAMQNSDGQFFALPIDTDVAGIVYNRTVLKD
                     AGIDPTTLTDWTAFETALKTLKDAGVTPVSSSGKDSWFAGNITDFIGSGDFSEKALDG
                     FKDGTFDEAGYTRILGHLSTWQKAGWINPDYSSATTDDLGRALAEGKTGFVFVQNYLV
                     ATALGFDPKADLGYMPIPSEDGKPFLVGGEGRAYGVSKTSPNKQQALDYLAYLAQPDN
                     LSELASSIGGIPGLTNAKSDLGILTASYDQFVKPGTLPLKPYFDRVYLPNGIWDTMVT
                     TTDGVLTGQMTPADAVKQMKSSYDSLRG"
     gene            11368..12576
                     /locus_tag="VN041_05405"
     CDS             11368..12576
                     /locus_tag="VN041_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU28515.1"
                     /translation="MAFTLGIVGAGQFAGQFATLFHHHPGVSAVHITDVIPARADELV
                     RAVGLAGTFASFDEMLASPEIDAVAIFTQRWTHGPLVVRALRAGKHVYSAVPMAISVD
                     EIAAIIEAVRETGLTYMMGETSYYNPSTVFARQKLAEGAFGRLFYGEGDYVHDMDLGF
                     YEAYQYSGGENWKATASYPPLLYPTHSIGGVLGAWPTHATAVSAIGVVDQRGDGVFDR
                     VVSQFDNDFSNATALFELAAGGSMRVNEFRRVGYPSHLRESRFRWFGTEGSFEQLALT
                     TVWQDKTGVEDISHLIDTHASVAHDDPSLANVAPALRDAFVSGYAAIHETERARIPAE
                     LIPLHNGHEGSHHFLVDDFVRAVNENTLPPVNAWQAARFTLPGVIAHESARRGGERLT
                     IPDMGDADAR"
     gene            12566..>13533
                     /locus_tag="VN041_05410"
     CDS             12566..>13533
                     /locus_tag="VN041_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28516.1"
                     /translation="MPAERRSRSVTRADVARYAGVSTAVVSYVLNDGPKAVAPLTKER
                     VLDAVRVLGYRPNAAARALSMGAADMFGLLVHDNRSPFFAELCHALDQATADAGRSLL
                     IVNSDRTQTSTSEQIRDLLSRQIGGLIIADVLTAGEQSLVESLGIPAVVIGQFGETDG
                     LHSVGVDFSVGAREATEHLIGHGYREIAFAGSAARYDARERGWREALTSAHLPLGPLV
                     HTPFTYQGGYDAAVELLDGAVRPRAVLAASDQIAIGMLTAFGERGIRVPDDIAIVSFD
                     GTVVADFVWPRLTTAVQPLEQMADAAVRLLLEPDAVRDHRAFRTRLR"
BASE COUNT         2161 a         4567 c         4672 g         2133 t
ORIGIN      
        1 atcacgtcga ccgccggcac ggcgcgggag agccaggtcg ccacggcgcg cgccacggtc
       61 agcagcgcgc gggcgatcag ctcaccccgg tgcagcgccg cgacggtcag agcggcggcg
      121 acagcgtgtg cgaaccacat cgcggcatcc tgcgggagga gcagcggggc atcgcccccg
      181 aacgacagcg gtacgacgcc gtgcacgtgg ggaccgaggc cgccctgcgg agtgatgctg
      241 cccagcacga agagcacgtg gaagagcagc tgggagatga tcaccgatgc gctcagcctc
      301 agcagcgaca gccgatgccc cgcgagcagg gtgcacgcca tcaacgacag cagccacggc
      361 acggcgatgc ccagcatccc gggcatgtag ccgccgaccc agacgtgcga ggccagtgcg
      421 acgaacgtcg cgatcgtggc gccgacgaac ccgcgcagca gcgggactct acgtgagggg
      481 cgcacccctc catcttttca cgcgtccagc gagcgctgcc gcgtgatcag ccggccttgc
      541 agatggcgcc gtcggcgatg gtctgcgcgt actgctccgc cgtgaagggc aggccgaagt
      601 ccggagccgt ctcgcccttg gcggccggcg ccttcgacgc gatcgcggcg agctcccagg
      661 ccaggtaggc ggcaacgccg ccacccgcgc tcgagttgtt cagggtgacc gccacggtga
      721 agccggtcgc agggtcggag tacgcggcgg tggcgtaacc gggaacccag ccgtgctggc
      781 cgaccatcga gccgaccagg tacgcgccgc cggtggcctg gtaccaggcc ggggcgtccg
      841 ccgaggccgg caggggcttc tcgaaccggc ccggcttggc gtccttgtcg cgcagcgcct
      901 ggcgagcctc ggcctgcgcg tagcggccca ggtcctcgat cgtggagacc gctccggagt
      961 cggtgaagcc gatgctcgac gagctcttgg tgatgtcgac cggtgcggcg caattgtagc
     1021 cgccgccctc cgcgggcgac aggtacgcgc ccttcagggc cggtgccggc tcgggctccg
     1081 cggctgccgg agacggcagc gaggtcgatt cgaggcccag cggagcggtc acgtacttct
     1141 tgatgagctg cgaggcggtc agccccgact cgcgctcgag ggcgaggccc agcatcaggt
     1201 agttcgcgtc ggagtcgcgg tacgccgtgt gagcggggcc gcgctgctgg ccgaggccga
     1261 agctcgccag ctgcagtggc gcccactggc ggtcaggggt gttcagccaa tacggcttcg
     1321 ccgtctccgc cgacgagccg acgccgctcg tgccgttgca caggtcgagc agcgtgatgt
     1381 cgcccaggtc cgcgacgccg gagacgtact tcggcacctt ggcgtcgagc ttgatgtcac
     1441 cggcatccgc catgccgtac agcacgtcgc aggtcatcat gcgggtgacc gccgcgatgc
     1501 ggaacgacat gtccttggtg accttggtct tgtcgcctgc ggcctgggtg ccgagacccg
     1561 tcacccattg gccactccac ggcacccaca cgcccacgac cgcccccgac gacccggtgg
     1621 cggccatcgc ctgctgcacg gcggtctgca gctgcgcggt cacgtcgtcg gggagcgccc
     1681 ccgcagcctg cttcggcggc gtgtaggtga gctcgggctg cgatgatccc gtgcatccgg
     1741 tgagagcgag agcgagggcg acggcgccga cgacggcggc acgcaggcga cgcgacgaac
     1801 gaagatgcat gtatcgactc tcccggggtt ggaccttccg agtctagagt gcggatgctg
     1861 ggagcacgcc cctcaggcct tcgcaacctg ctcggcctgc ctggcctccc aggcctcccg
     1921 catgaagcgg cgcacgtcct cgtccacctg gatgtcgcgc ggccgcagcg gccgggacag
     1981 gtacaggccg tcgagcgagg tgagtctcga cagcgccaca taggtctggc cgggcgcgaa
     2041 cgcccccgac ccgagatcga cgatggcccg atcgtaggtc ttgccctgcg acttgtggat
     2101 cgtcaccgcc cacgccagtc gcagcgggaa ctgcgtgaac tcggcgacca cctcgcggga
     2161 gagcgtcttg gtgcccgggt tgtaggcgta ccgcagcctc tcccagaccg cgggctcgac
     2221 gtcgaactcc tcgccgtcga tgtccacccg cacggtgccg ccgaggatgc gggtgaccgt
     2281 gccgatcgag ccgttcaccc agcgcggtgg ctcgccctgc atgcccacgt cgttgcgcag
     2341 gaacatcacc tgcgcaccga ccttgagctt cagctcggca tccgccgggt agttgccctc
     2401 gccgcgaccg aaatcgccgc tgacctcggc gtgcgcggtc tgctcgcgac cggcgagagc
     2461 ggcgagatgg cggctgttga tgctgttgac gatgtcgttg cgggttgcga gcgtgatgat
     2521 cggcggctca cccggctcgg ggtcgggagg cgtgcgtgcc ccgcgctcgt tcagcacgtc
     2581 tgcgatgtcc ttggtcaccc ggccgtaccg caccgcgttc agcatcgact tgaacccgtc
     2641 gtcggactgc ctgtggatct gcaccagctc gtgcacgtgc agcttcgcgc cgtgctcgcc
     2701 gatgtccagc atcccgccgc cgtctccggc caggccgccg gtccagacct tcgcgtcgaa
     2761 gaaccagaac gaccggtagt ggtcctggat gtagcgcagc tcgtcgcccc gcggtggcac
     2821 gggcgccagc tggtaggggt ctccgaacat gaccacctgc acgccgccga acgggatgcc
     2881 gcgcttgccc cgcgcctggc gcagagacct gtcgatcgcg tccatcaggt cggcgttcac
     2941 catcgagatc tcgtcgatca cgagcgtctg gatggcgttg aggatgcggc gggtggcatc
     3001 cgactgctcg atctcgctct cggcgatcag cccgatcggc aggcggaaca gcgagtggat
     3061 cgtctgaccc tcgacgttca gcgcggccac acctgtcggc gcgcagatcg cgatctgctt
     3121 ctgggtgttc caggcgaagt gctgcagcag ggtcgacttg cccgtgccgg cccggcccgt
     3181 gatgaacacg tgctcatcgg tgtcctcgat cagacggaac agcgcctgct gttcatcgga
     3241 tagggtcacg gacacgcacc ccatgttaag tcctctgtgt cgccgctcgg ctccgtccca
     3301 gcccccgctc cctagacttg tcggcatgat gcagggtcgg ggggtgtcac agcgacgttc
     3361 tgcactccgc accgacctga cgatcctcgg ggtcgtgggg gtgctgctca tcgctgcgct
     3421 cggcgccggg ggcatgtcgc tgtaccagca ggtgtacagc ccgtccgcct tcgtgcagcg
     3481 ctatctcgac ctgctcgcct ccggccgtgc ggccgacgcg cttcaggtgc cgggcgtctc
     3541 ggtggacctg tccatcaaga aggacaccga catccatgcc accgcatccg aggcgctgct
     3601 gcgcaccgcc gccctcgcgc agctgaccga ctacacggtc acctccgaga cgcagaaggg
     3661 cggcgtctac cgcgtctcga ccgcgtaccg tgcgggcggc gtgcgcggca cctccaccta
     3721 ccgggtggtg caggacggct gggtgggcgt ggtccccaac tggcggttcg agcgcagccc
     3781 gatcgccgag gtcgagctca ccgtgcgcgg cgcggacgtc ttcaaggtga acggcttcga
     3841 actggaccgc cgccaggtgt cggacaaggg ggtaggcgct tcgccgctcg atccgctgca
     3901 tctgctggtc ttcactcccg gggcgtactc ggtgaccgtc gacacgccga tcgccgcgac
     3961 ccccggcgtg cgcgtgctcg cggacaaggc gctcgcgcac acgccggtcg acgtgcaggc
     4021 gcagccgacc gccaagttca ccgagctcgt gcagcagcgc gtcggggagt tcctcacggc
     4081 atgcgccacc cagcaggtgc tgctgccgac cgcgtgcccg ttcgggctcg aggtctccaa
     4141 ccggctcgcg gacggctcgc tgccgaagtg gtcgatcacg cagcagcccg tggtgaccgt
     4201 ggttcctgac ggcgcgaact ggaagatccc cccgaccgat gcggtcgcgc acatcgtggt
     4261 ctcgatcaag tcggtcttcg acggctcggt gcgcgaggtg tccgaggacg tgccattcca
     4321 gatcgacggc acgatcagcg tccagcccga cggcaccgcc tccatcaggg tcggctcgcc
     4381 gaacatcgac gccgagtagc cgcgctgcgg ccgcagcggg tcaggaccgc gatgcggcgc
     4441 gacggtcctg ctcggccagc ttcgagagcc gggcgttgta ttcctcgagc tcggcgtccc
     4501 cgtcgcggtc ggcggcgcga tcgctgcggc gctgcagctt gtcatccgag cgcgaccact
     4561 ggatcgcgac ggtgatcgcg aggatcagcg tggggatctc acccaccgac caggcgatgc
     4621 cgccgccgat gtactgatcc tccatgggcg tcgggcccca ggtgcggccc atcgagccga
     4681 accattcggc gaccatgagc ccgttctgca tcatcagcgc catgccgaag aacgcgtgca
     4741 tggccatcac ggcgatcagg gtgatcaggc gtcccgcgtg cggcaggcgg tacggcaccg
     4801 ggtcgacgcc gaccagcgac atcacgaaca ggtagccgga gatgaggaag tggatgacca
     4861 tccactcgtg cccgagatgg tcgtacagcg accaccgcac caggtcggtg aagtagaagg
     4921 cccacagcga gacgatgaag atcgcggcag cgacgatcgg atgcgtgatc acccgggaga
     4981 acggcgagtg caccgcccac atgatccact cgcgggcgcc gcgggtgccg tcctcgcgct
     5041 tgtggatcgc gcgcagcgcc agcgtgatcg gcgcgccgct gaccagcagc agagggatcg
     5101 ccatgctcag caccatgtgc ccggccatgt gcacgctgaa caggtactcc tggtacgcgt
     5161 tgagcgggcc cgacgtgacc cagagcagca ggagcatccc cgccagccag aagatcgtgc
     5221 ggtgcacggg ccacgcgtcc ccgcgccggc gcaggcggac gacgccggcg atgtagaaga
     5281 acaccgcgaa gccgatcacg accgcccaga gcacgtcgat gtcgtaggac gtgaaccagc
     5341 ggtcgagggt cagctcggga gggaggggcg aatcggtgag gcgctgagcc gccgtcgggt
     5401 tggcgggagc ctgctcgccg acaggcggag gcgtgcgcgc cagggccgcg gcggcgccgg
     5461 atgccaggcc catcagcagc agctcgccca ggatgagcgt ccagaacgac cgcttctcgt
     5521 ccgcgccgct gaaccggggg atgagccggc ggcggtacca ggcgccgaac aggcccaggc
     5581 acagcagcag gatcgccttg ccgagcacga tcaggccgta gggcgagaac agctcgctcc
     5641 agctgccgag cgccacgacg gtgcgggcga tgcccgagac ggcgacgacg atgaaggcga
     5701 tcagcgcgat cgtggagtac cgctcgacca ggaccggcag atccagggca tccaccttct
     5761 tcgccttgcc gttcgtccgg ttcgcggcgc tgcgcaggat gatcagcaga aggatgccgc
     5821 cgacccaggc ggcggctccg agcgtgtgca gcaggatcgc gttgaccgcg atgttgtggc
     5881 cctccacgtc gccggagtgc ccctgcgtcg caagcgggag gaaggaggcc gcggcgagcc
     5941 cggcggtcat gagcgtgccg gtccagctgc gccagccgaa ggccagcagc gtgatcacgg
     6001 cgcccatcag cacggtgatc agccaggcct gaccgagcgg gagcgtgagc aggaacgtgc
     6061 cgagctgctc gccgaaggtg cggtcggccg agagcttggg attgaaggcc gcgagcagcg
     6121 tcatcccggc ggcgatcgca gagctgaccg tgaagatcgc cgcgcccacg gatgccgtgt
     6181 cgagggcgat gtcgaagggg cgcgaaccgc tgcgcagccc gaacagcgcg agcagcaggg
     6241 agcccagcat ggccgcgccc gcgaggttgg cgaccagctt gacggccggc agaccccaca
     6301 tcacgatggg tcccgggtcc aggagcttgg gcggcacggc gccgccgccg acgacgagcg
     6361 ccgcgatcag gccgaacgcc gcgccggcga ggaggatcgc gaggccgacg agacggtacg
     6421 acttcacccc tccagcctac ggcggttgcc tatgaccgcg gatccgaggc cgcacgtgcg
     6481 gaccgcgggc aggcgtgccc ggctcgcgtg ggatcggcac gcgccaggca cgcagaaggc
     6541 cgcccccgca gggacggcct tcgtggtggt gcggtcttac ttgacagcag ccttcagctt
     6601 ggagccggcg gtcaccttga cgcgcttgcc agcggggatc ttgatctcgg cgccggtctg
     6661 cgggttgcgg ccggtgcggg cggcggtggc cacctgctcg aacgagatcc agcccgggat
     6721 cgagaccttg ctgcccttgg cgacagcgtc ggagacggtg ccgaacagcg agtcgaggac
     6781 gcgcgaaaca gtggcctggc tctcgccggt ggcggaagcg atgctcgcga cgagctcggt
     6841 cttggtgatg gacttgtcag ccatgtcatc ctccagcgac gacggtccgt cgcatcgtgt
     6901 tgatttcgga gttccccccg tcggtcgatc gaccgcctcg aatgtatcaa ccacaaccga
     6961 caaaaccgcg tcatttcgcg ggttttggca tgtttctcgg cgtttccatt cacttcagtc
     7021 acatcagtca caggttcgcg tggtcaccgg catccgtccg gcccggcccc gcatccgaaa
     7081 acaggagaac agcccgggac aggagaatcc catgcagcac cctcatgttt cgggcgatcc
     7141 tcctgttccg ggcgacacgc acacccggac acggactcca ggtacgcgaa aggggcgagg
     7201 atccgaagat cctcgcccct cacagcggat gcttaccagc tcgacttggt gataccgggc
     7261 agctcgccac ggtgcgccat gtcacggaag cggacacgcg agatgccgaa cttcgtgagg
     7321 acaccacggg ggcggccgtc gatggcgtca cgcgaacgca cgcgaaccgg cgacgcgttg
     7381 cgcggcagct tctgcaggcc gatgcgggcg gcctcgcggg cctcatcggt ggcgttcggg
     7441 tcgaccaggg tcttcttcag ctcggcacga cgctccgcgt agcgggcgac gatgaccttg
     7501 cgctgctcgt tcttggcgat cttgctcttc ttggccatgt cttaacgctc ctctcggaat
     7561 tcgacgtgct tacggaccac cgggtcgtac ttcttcagca cgaggcggtc gggggtgttg
     7621 cgacggttct tcttggtcac gtaggtgtaa ccggtgcccg ccgtcgaacg gagcttgatg
     7681 atcggacgga cgtcctgtgc cttcttggcc attagagctt cacacccttc gccttgaggt
     7741 cggcgacaac cttctcgatg ccgcgtgcgt cgatcacctt gatgcccttg gcggacacgt
     7801 tgagcgtgat cttacgaccg agcgacggga cgtagtaagt cttcttctgc acgttcgggt
     7861 cgaagcggcg cttcgtccgg cggtgcgagt gcgagacgtt gtgaccgaag ccgggaacag
     7921 caccggtcac ctggcacact gctgccatgt tgtgactcct tctataccgt gaggccggac
     7981 ggcctcaccc aagatccctt gtctgcatcc caccctcgaa cacggccaca gggccgggag
     8041 gaaggagtgg gacacgaact gcgcacagga gtgtgcacag acaaggagtc agcttaccag
     8101 aaccggatca cttctccgaa ccggccacca tgcccttgat gaagtacttc tgcagcacca
     8161 ggtagacgat cagcaccggc agcgcggaga gcagggatgc cgcggctgcg gcgcccacgt
     8221 cgctcgacgt ggtggagaag aacgtcgcgg tggccggcgc gatcgtgcgc atctccgggc
     8281 tgcgcatcag gtagacgctc agtgcgtagt cgttccacac gttcacgctg gcgaggatca
     8341 cgaccgtggc ggtgacgggc ttgagcatcg ggaccaccac gcggaacagc acctgcacgg
     8401 cgttcgcgcc gtcgaccgac gcggcctcct cgatcgagat cggcaacccg cgcatgaacg
     8461 cggtgtacag gaagatcgcc agcggcagct gcgtggcggt catcaccagg atcgtgcccc
     8521 agtaggtgtt gatggcgtgc cactggctca tcatcgagta cagcggcacc aggatcgaca
     8581 gcggcgggat catgatgagc cccaccacgc cggccagaac cagcttgttg ccgatcgtgg
     8641 gtcgccgagc cagcgggtag gcggcaaggg cgccgagcac gcacaccagc acggtggcac
     8701 cgagagtgac gatcgcgctg ttgccgatcg cacgcaggat gccgccctgc tcgatcgcgg
     8761 tggcgaagtt ctgccagtgg atgccggaga acggcggcag ccactgcgag gacaggtcgc
     8821 tgcgcgcctt catcgccgag gtgacggtga tgtagaacgg cagcagctgc agcagcaccg
     8881 cgagcagcac gcccgccccg atcgcgaggc gcgcgggcca cggcgagcgc ggggcggatg
     8941 ccgtgcgccg ggcgtcgccg gtcaccacgg atgccgtgct cgtcgcgctc acagctgctc
     9001 cagccttcgc cggttgagcg cggtgttcag cacgagggtg aacaccgcga tgatcaggaa
     9061 cagcaccaca cccatcgccg aggcgtagcc ggcgcgctga ctgccgaagt acatcttggc
     9121 gatcagcgtc gacacggagt tcgtggagta cccgggaccg ccggcggtga gcacctggat
     9181 cacgtcgaac agcttcatgc cgccgatcag attgagcacg atgctcgtcg cgaacgccgg
     9241 ctgcagcatc gggagcgtga tgctgcggaa cgactgcgcg gcatcggcgc cgtcgagtcg
     9301 cgcggcctcg tagtacatgg tggggatcga ctgcaggccc gcgaggtaga tcaccatcga
     9361 gatgccgacg aactgcagcg agttcaccac cacgatcagc gcgatcgccg taccggaatc
     9421 gctgagccac gcggtgcgct caccgccgag cgcgatgacc aggtcgttga gcccgccgcc
     9481 gttgtaggcg aggaacaggt agtacatcgt gcccatcacg atcggcgaga cgagcacggg
     9541 caggtagatg atcgcccggg cgacgccgcg ccctcgcacg tggcggtcca gtgcgagcgc
     9601 gagtcccagt ccgatcacct gctgcagaac ggtcgacccg atgccgtaca gaaaggtgtt
     9661 cccgagcgcg gtgcggaagt tcggatcctc gaacagccgc agatagttcg cggggccgac
     9721 gaaggaccgg tcgggcgagt agccgtccca gttcgtcagt gacagcagca cgccgttcag
     9781 caggggccac agcatgaaca cgccgagcac ggcgagcgca ggcaggtaca gcaggttgag
     9841 gccgcggctc gcgaaacggg ggaccaaccc ctcggtcccc cgtttcggcc tgtgcaccgt
     9901 cctgacggtg gtcgtcatcc gcgcagcgag tcgtagctcg acttcatctg cttcacggca
     9961 tccgccggag tcatctgccc ggtgagcacg ccgtcggtgg tggtgaccat ggtgtcccag
    10021 atgccgttgg gcaggtagac gcggtcgaag tacggcttga gcggcagcgt gcccggcttg
    10081 acgaactggt cgtagctggc ggtgaggatg ccgaggtcgc tcttcgcgtt ggtcaggccc
    10141 gggatgcctc cgatcgagct ggcgagctcg gagaggttgt cgggctgggc gaggtaggcg
    10201 aggtagtcca gcgcctgctg cttgttcgga gaggtcttgg agacgccgta cgcacggcct
    10261 tcaccgccga ccaggaacgg cttgccgtcc tcggagggga tgggcatgta gcccaggtcg
    10321 gccttcgggt cgaagcccag ggcggtggcg accaggtagt tctgcacgaa cacgaagccg
    10381 gtcttgccct cggcgagcgc acgcccgagg tcgtcggtgg tggccgagga gtagtccggg
    10441 ttgatccagc cggccttctg ccaggtggag aggtgcccga ggatgcgggt gtatccggcc
    10501 tcgtcgaagg tgccgtcctt gaacccgtcc agcgccttct cgctgaagtc acccgagccg
    10561 atgaagtcgg tgatgttgcc cgcgaaccag ctgtccttgc cggatgacga gacgggggtg
    10621 accccggcat ccttcagcgt cttcagcgcc gtctcgaagg cggtccagtc cgtgagcgtc
    10681 gtcgggtcga tgccggcgtc cttcagcacg gtgcggttgt agacgatgcc ggcgacgtcg
    10741 gtgtcgatcg gcagcgcgaa gaactgtccg tcggagttct gcatcgccgg ggccagcgcc
    10801 ttgttgaagt ccttcgccca gggctgatcg tcgagcggct cgaggaactt gctgtaccga
    10861 agcagggacc acccgtgcgt ggcccagatg tccgggatgt cgttcgacga gagccgcacc
    10921 ttgatgtcgt cctcgtaggt gtcggtggag gggaccagct cgatcttgac ccccggattg
    10981 tccttctcgt aggcgtcggt gatcttcttc agcaccttca gcgagggatc ggtcgcgccg
    11041 aagttggtct gcatctcgat cttgacgtca cctccggtgc cgctggagtc gcccgagcat
    11101 ccgctcaggg cgagagccgc ggtcgtggcg accgccgcgc ccgccaggag ccgcttggaa
    11161 ttcagggatg tcttcatgtc tggtcctcac tgtcctcggc gtcgctgtcg atggcggaaa
    11221 aaactgatgc gcattcgtcc tgttcgtgat acgttacccg cgtagatcac tggggtacag
    11281 ggggtctcgg aatcgttctg aggggcacga ttccggggcc tcctttcatg ttgacacccg
    11341 tagacccgcg atggaagact cgggcgcatg gcgttcacct tggggatcgt cggcgcgggc
    11401 cagttcgctg gccagttcgc gacgctcttc caccatcatc ccggcgtgtc cgcggtgcac
    11461 atcaccgatg tgatccccgc acgtgccgat gagctcgtgc gcgccgtggg cctggccggc
    11521 accttcgcat ccttcgacga gatgctggcg tcccccgaga tcgacgcggt cgccatcttc
    11581 acgcagcgct ggacgcacgg tccgctcgtg gtcagagcgc tgcgcgcggg caagcacgtc
    11641 tactccgcgg tgccgatggc gatctcggtc gacgagatcg ccgccatcat cgaggcggtc
    11701 cgcgagaccg gcctgaccta catgatgggc gagaccagct actacaaccc gtccaccgtg
    11761 ttcgcccgtc agaagctcgc cgagggcgcc ttcggtcggc tcttctacgg cgagggcgac
    11821 tacgtgcacg acatggacct cggcttctac gaggcgtacc agtactcggg cggcgagaac
    11881 tggaaggcca ccgccagcta cccgccgctg ctctacccga cccattcgat cggcggggtg
    11941 ctcggcgcct ggcccacgca cgccacggcg gtctcggcca tcggcgtcgt ggatcagcgc
    12001 ggtgacgggg tgttcgaccg cgtagtgagc caattcgaca acgacttctc caacgcgacc
    12061 gcactgttcg agctcgccgc gggcggatcg atgcgcgtca acgagttccg ccgcgtcggc
    12121 tacccctcgc acctgcgcga gtcacggttc cgctggttcg gcaccgaggg ctcgttcgag
    12181 cagctggcgc tcaccacggt gtggcaggac aagaccggcg tggaggacat ctcgcacctc
    12241 atcgacacgc atgcgtcggt ggcgcacgac gatccgtcgc tcgcaaacgt ggcccccgcg
    12301 ctgcgcgacg cgttcgtttc cggctacgcc gcgatccacg agaccgagcg cgcccgcatc
    12361 cccgcggagc tcatcccctt gcacaacggt cacgagggca gtcaccattt cctcgtggat
    12421 gatttcgtgc gggccgtgaa cgagaacacg ctgccgccgg tgaacgcctg gcaggcggcc
    12481 aggttcaccc ttcccggcgt gatcgcgcac gagtccgccc gtcgcggcgg ggagcgcctc
    12541 acgatccccg acatgggaga cgcggatgcc cgctgagcgc cgctcgcgct cggtgacccg
    12601 cgccgacgtg gcccgctacg ccggcgtgag cacggcggtg gtcagctatg tgctcaacga
    12661 cggaccgaag gctgtcgccc cgctcacgaa ggagcgggtg ctcgacgccg tgcgggtgct
    12721 gggctaccgt ccgaacgccg ccgcccgcgc gctgtcgatg ggcgccgccg acatgttcgg
    12781 cctgctcgtg cacgacaacc gaagcccgtt cttcgccgag ctctgccatg ccctcgacca
    12841 ggcgacggcg gatgccggcc gatccctgct gatcgtgaac tccgaccgca cgcagacctc
    12901 cacgtccgag cagatccgcg acctgctgtc acgccagatc ggcgggctga tcatcgccga
    12961 cgtcctcacc gcgggcgagc agtcgctggt ggagtcgctc ggcatccccg ccgtcgtgat
    13021 cgggcagttc ggcgagaccg acggtctgca cagcgtcggc gtggacttct cggtgggtgc
    13081 ccgcgaggcg accgagcacc tgatcggaca cggctaccgc gagatcgcgt tcgccggcag
    13141 cgcggcccgg tacgacgcgc gcgagcgcgg ctggcgcgag gcgctcacct ccgcgcacct
    13201 gccactgggc ccgctggtgc acaccccgtt cacctatcag ggcgggtacg acgcggcggt
    13261 ggagctgctc gacggggcgg tacggccccg ggcagtgctg gcggcatccg atcagatcgc
    13321 gatcggcatg ctcaccgcct tcggcgagcg cggcatccgc gtgcccgacg acatcgcgat
    13381 cgtgtccttc gacggcaccg tcgtcgccga cttcgtctgg ccgcggctca ccacggcggt
    13441 gcagccgttg gagcagatgg cggatgccgc ggtgcggctg ctcctggagc cggacgccgt
    13501 gcgcgatcac cgcgcattcc gcacgcggct gcg
//
LOCUS       DATMSX010000176         5135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_88031,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000176 DATMSX010000000
VERSION     DATMSX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88031"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VN041_05415"
     CDS             complement(<1..427)
                     /locus_tag="VN041_05415"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWU28517.1"
                     /translation="MTLEPTNTEAIRVITRFEASNGIPDSMRSDVRMLGGLLGQVLRE
                     SGSPGLFEDVERLRLATIQAADADDEAFARAASIADSFTIERADEVARAFTCYFHLVN
                     LAEEHQRVRILRERAGQPGREDAADTVATAFAQLSREVGE"
     gene            532..1695
                     /locus_tag="VN041_05420"
     CDS             532..1695
                     /locus_tag="VN041_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWU28518.1"
                     /translation="MRISANPLRGQQFPSALLLAAAALGLLLANLSTHDGIAAVLEAH
                     IAIPGTALDLSVEEWVADGLLAIFFLVVAIELRHELTHGELNSVRKAVQPAIAAAGGV
                     IVPIVVYLLIAGGGETHVGWPIPTATDIAFALGVLAMFGKGLPAAVRVFLLALAILDD
                     IIGIIFIAVLFAHDVNWLMFALSVVAVAVFWALSHWLHATGHSGIAVAMGVVGVIAWG
                     LMAVSGIHATIAGVLLGLVMAPVPAERVRHALEPTVNGLILPVFAFVAAFVVIPQVSP
                     AQLSPAFWGILIALPVGKVVGITLFGWLSLQLRPRGTAPALVLPDLIAAGVLGGIGFT
                     VSLLLANLAFAGGGDIRDEAILGVLAGSLIALALSGVIVSMRARHYRMLAKSA"
     gene            1766..2374
                     /locus_tag="VN041_05425"
     CDS             1766..2374
                     /locus_tag="VN041_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HWU28519.1"
                     /translation="MRQVRERCVWSQRITHRDLVPYLIEESHEVIDAVEAGDRAELRE
                     ELGDLLWQVLFHSAVAAQDADEPFDIDDVARGLTEKMVRRHPHVFGDAVATTPEEVLV
                     HWNAAKAAEKRERRSVLDGVPAGMPALARAQKVLGRAAKVIGSDPGPESVEGPALASE
                     SALGDALLALVAQARENGWDAERALRERLRRLEDEVRAAEAG"
     gene            complement(2456..3025)
                     /locus_tag="VN041_05430"
     CDS             complement(2456..3025)
                     /locus_tag="VN041_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28520.1"
                     /translation="MSAAPMLAHPSADRFTEWVELVEDYGGPESTELVGSGFHGNREP
                     RVPVLTPDGYATWLAMLAEEADESIPPHPGRVKSSWFWILDDDGAWVGFLALRRSLNA
                     ALLETGGHIGYSIRPSRRGEGLAGRALADALVEASAIGLERVLITCDVQNTASARTIE
                     RNGGVYEDSRGGKRRYWVPTGPKRTMGGG"
     gene            3172..4449
                     /locus_tag="VN041_05435"
     CDS             3172..4449
                     /locus_tag="VN041_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWU28521.1"
                     /translation="MRDFLPADKARRERVLAVIRERYRAHGFDEIETPVVEEYDRLHA
                     GIGGDNEKLSYNILRRGLDADAIREAADDPAALSDLGLRYDLTVPLARFYATNRGQLP
                     AVFRSIQIGPVWRAERPQKGRYRQFVQCDIDIMGDESARAEAELIVASLDTIDALGLE
                     GGMVRVNDRRALEWMLDSFGFTEDERPGVLITIDKLDKIGPDGVAAELRERGATASAV
                     DAFAAFLVRPQTMEYNPYGERQIRKALPEGAPDELVAHLVGLGDAVVAARPDAGEPPL
                     VYDPFLVRGMGYYTGTIFELSHPSVGYSLGGGGRYDGMIGRFLGQQVPAVGFSIGFER
                     IVDLVAEQEADAAQAIVLVHDADVPVSELLAHKAAVLRSLSPSKGGGRVRLERRPKNL
                     KGLLERSAADGYTGFVSVRAGDEPGSAEVKPLA"
     gene            4581..>5135
                     /locus_tag="VN041_05440"
     CDS             4581..>5135
                     /locus_tag="VN041_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU28522.1"
                     /translation="MNSVRPALLTVAVLAAAAIGVRFGLARQAAIARRRIGKPLGEDA
                     LAVDRVWRRRLPGEPIDLLIVGDSLAAGLGASRRKETLGGRLAKGLAKRTGRPVRLRS
                     LAVVGSESPDLAAQIDRLTPEDRVDVAVIVVGGNDVTHRIPVAESVGHLVAAIQRLQL
                     RGARVVVGTCPDLGALRPVPQPLRT"
BASE COUNT          706 a         1813 c         1819 g          797 t
ORIGIN      
        1 cctcgccgac ctcgcggctc agctgagcga aagccgtggc gaccgtgtcg gctgcgtcct
       61 cgcggcccgg ctggcctgcg cgctcgcgca ggatccgcac gcgctgatgc tcctcggcga
      121 ggttgaccag atggaagtag caggtgaagg cgcgcgcgac ctcgtcggcg cgctcgatcg
      181 tgaacgagtc ggcgatggac gccgcgcgcg cgaacgcctc gtcatcggca tccgccgcct
      241 ggatggtcgc cagacgcagc cgctcgacgt cctcgaacag gccggggctg cccgactcgc
      301 gcagcacctg accgagcagc ccgcccagca tgcgcacatc cgagcgcatg ctgtccggaa
      361 tgccgttgga cgcctcgaaa cgggtgatga cgcggatggc ttcggtgttc gtcggctcga
      421 gggtcatgtt cccgagcgta gcccgggcat gtgcggcatc cgttcttcgt gacaggcgat
      481 cggatgcccg tctccgcagg ccgcggaacc cgacgactta cgatggaggg gatgcgcatc
      541 tcggcaaacc cccttcgtgg tcagcagttc ccctccgcac tcctcttggc cgctgcggcg
      601 ctcgggctgc tgctggccaa tctgtccacg cacgacggga tcgccgcggt gctggaggcg
      661 catatcgcga tccccggcac cgcgctcgac ctgtccgtcg aggaatgggt ggccgacgga
      721 ctcctggcga tcttcttcct ggtcgtcgcg atcgaactga ggcacgagct cacccacggc
      781 gagctgaact cggtgcgcaa ggccgtgcag cccgcgatcg ccgccgccgg cggcgtgatc
      841 gtgccgatcg tcgtctacct gctcatcgcg ggcggcggtg agactcatgt cggctggccg
      901 attcccaccg caactgacat cgccttcgcg ctcggcgtgc tggccatgtt cggcaaggga
      961 ctgccggcgg ccgtgcgcgt gttcctcctc gccctcgcga tcctcgacga catcatcggc
     1021 atcatcttca tcgccgtgct gttcgcgcac gacgtgaact ggctgatgtt cgcgctctcg
     1081 gtggtcgccg tcgccgtgtt ctgggcgctc agccactggc tgcacgccac gggtcactcc
     1141 ggcatcgccg tggccatggg cgtggtcggc gtgatcgcct ggggtctcat ggcggtctcc
     1201 ggcatccacg ccacgatcgc cggcgtgctg ctcggactcg tgatggcccc cgttcccgcg
     1261 gagcgcgtgc ggcatgcgct ggagccgacg gtgaacgggc tgatcctgcc ggtgttcgcg
     1321 ttcgtggcgg ccttcgtcgt gatcccgcag gtgtcgccgg cgcagctgtc acccgcgttc
     1381 tggggcatcc tcatcgctct gccggtcggc aaggtggtcg gcatcacgct gttcggctgg
     1441 ctgagtctgc agctgcgccc gcgcggaact gcaccggcgc tggtgctgcc ggatctgatc
     1501 gccgccggcg tgctcggcgg aatcggattc acggtgtccc tgctgctcgc caacctcgcg
     1561 ttcgccggcg gtggcgacat ccgggacgag gcgatcctgg gtgtgctggc cggttcgctg
     1621 atcgccctgg cgctgtcggg cgtgatcgtc tcgatgcgcg cgcgtcacta ccggatgctg
     1681 gcgaagagcg cctgaggagg acgccgtgac cgaccccgat ccgctgcgcg acgtgtccga
     1741 cccgctgcgc gccgcggccg agaccatgcg gcaggtgcgg gagcgctgcg tgtggtcgca
     1801 gcggatcacg caccgggacc tcgtgccgta cctgatcgag gagtcgcacg aggtgatcga
     1861 cgccgtcgag gcgggcgatc gcgcggagct gcgcgaggag ctcggcgacc tgctctggca
     1921 ggtgctgttc cactccgccg tcgccgcgca ggatgccgac gagccgttcg acatcgacga
     1981 cgtggcccgc ggcctcaccg agaagatggt gcgccggcat ccgcacgtct tcggcgacgc
     2041 ggtggccacc acgcccgaag aggtgctcgt gcactggaac gcggccaagg ctgccgagaa
     2101 gcgcgagcgg cgaagcgtgc tggacggcgt gcccgccggc atgccggctc tggcacgcgc
     2161 gcagaaggtg ctcgggcggg cagcgaaggt catcggctcc gacccgggcc ctgagtccgt
     2221 cgaagggccc gccctcgcat ccgaatccgc ccttggcgat gcccttctcg ctctggtggc
     2281 ccaagcccgc gagaacggct gggacgcgga gcgcgccctg cgcgagcgcc tccgccggct
     2341 cgaggacgag gtccgcgcgg cagaggcagg ctgagcctct ccgcagtacg cgggccgagc
     2401 tccgcgaaga ggatgcaaga aggaaaacgt cgtcggagag cctccgaaac cggccttatc
     2461 ctcctcccat cgtccgcttc ggccccgtcg gcacccagta ccggcgcttc ccgccgcgag
     2521 agtcctcata gacgccgccg ttgcgctcga tcgtccgcgc cgaggcggtg ttctgcacgt
     2581 cgcaggtgat cagcacgcgc tccaatccga tcgcggaggc ttcgacgagt gcatcggcca
     2641 gagcccgccc ggcaaggccc tcgccacgac gtgacggacg gatcgagtag ccgatgtgcc
     2701 cgccggtctc gaggagcgcg gcgttcaggc tgcgtcggag ggcgaggaac ccgacccacg
     2761 caccgtcgtc gtcgagaatc cagaaccacg agctcttgac ccgccccggg tggggtggga
     2821 tggactcatc cgcctcctcg gcgagcatcg cgagccaggt cgcatagccg tccggggtga
     2881 ggaccgggac ccggggctcc cggttgccgt ggaatccgga ccccacgagc tcggtcgact
     2941 ccgggccgcc gtagtcctcg accagctcga cccattcggt gaaacggtcg gcggagggat
     3001 gggcgagcat cggcgccgcg ctcattccgt ggcaggcgcc gcgacgggcg ggcgcgcatc
     3061 gttctcgggc atccttccac tatgtcaaaa cgtgcgtgag cacccgtcgc acaggacacc
     3121 gctcgcacct ggaaagatgg acgggtggca aacgtgaacc cgccgcgcgg aatgcgcgac
     3181 ttcctccccg ctgacaaggc ccgacgcgag cgtgtgctcg ccgtcatccg cgagcgctac
     3241 cgcgcgcacg gattcgacga gatcgagacc cccgtggtcg aggagtacga ccggctgcat
     3301 gccggcatcg gcggcgacaa cgagaagctg tcgtacaaca tcctgcgccg cggactcgac
     3361 gcggacgcga tccgcgaggc cgccgacgac ccggccgcgc tcagcgacct gggcctgcgc
     3421 tacgacctga ccgtgcctct cgcgcggttc tacgccacca accggggtca gctgcccgct
     3481 gtcttccgct cgatccagat cggccccgtg tggcgtgccg agcggccgca gaagggtcgc
     3541 taccgccagt tcgtgcagtg cgacatcgac atcatgggcg acgagagcgc ccgcgccgag
     3601 gccgagctga tcgtggcatc cctcgacacc atcgacgcac tcgggctcga gggcggcatg
     3661 gtgcgggtca acgaccggcg cgcgctggag tggatgctgg actccttcgg cttcaccgag
     3721 gatgagcgtc cgggtgtgct gatcacgatc gacaagctcg acaagatcgg gcccgacggc
     3781 gtggcggccg agttgcgcga gcggggtgcg acggcatccg cggtcgacgc cttcgccgcg
     3841 ttcctcgtgc gcccgcagac catggagtac aacccctacg gtgagcggca gatccgcaag
     3901 gcgctgcccg agggtgcacc cgacgagctg gtcgcgcacc tggtcggact cggcgacgcg
     3961 gtcgtcgccg cccgcccgga tgccggtgag ccgccgctcg tctacgaccc gttcctggtg
     4021 cgcggcatgg gctactacac cggcacgatc ttcgagctct cgcacccctc ggtgggctac
     4081 tcgctgggcg gcggcggacg ctacgacggc atgatcgggc ggttcctcgg ccagcaggtc
     4141 cctgcggtcg gcttctcgat cggcttcgag cggatcgtcg acctggtcgc cgagcaggag
     4201 gcggacgccg cgcaggcgat cgtgctcgtg cacgacgccg acgtgccggt ctccgagctg
     4261 ctcgcgcaca aggcggcagt cctgcggtcc ctgagcccgt cgaagggcgg cgggcgcgtg
     4321 cgcctggagc gccgcccgaa gaacctcaag ggtctgctgg agcgctccgc cgccgacggg
     4381 tacaccggct tcgtctcggt gcgcgccggt gacgagcccg gctcggcgga ggtcaagccg
     4441 ctggcctgag cggcatccgc tggtccaggc gccgcactct gggggccgac acgtgacgga
     4501 tgccgggctg ccggcgtctt ccgaagaccc gtcctccggt gttcatgcgc cgttcacgcc
     4561 cggacgctgg gatcggatgc atgaactccg tccgcccagc gctcctgacc gtcgccgtgc
     4621 tcgccgcggc cgccatcggc gtccgcttcg gcctcgcacg gcaggcggcg atcgcccgtc
     4681 gtcgcatcgg caagccgctg ggggaggacg cgctcgcggt ggatcgcgtg tggcggaggc
     4741 gcctgcccgg tgaaccgatc gatctgctga tcgtcggcga ctcgctcgcc gcggggctcg
     4801 gagcgagccg gcggaaggag acgctcggcg ggcggcttgc gaaagggctc gcgaagcgga
     4861 ccggccgccc cgtccggctg cggtcgctcg ccgtggtcgg atcggagtcg ccggacctcg
     4921 ccgcccagat cgaccggctg acgcccgagg acagggtgga tgtcgccgtg atcgtcgtcg
     4981 gcggcaacga cgtgacgcac cgcattcccg tggccgaatc ggtcggacat ctcgtggcgg
     5041 cgatccagcg actgcagttg cgcggtgcgc gggtcgtcgt cggcacctgc ccggacctcg
     5101 gtgcactgcg gcccgtgccg caaccgctgc gcacg
//
LOCUS       DATMSX010000177         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135074,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000177 DATMSX010000000
VERSION     DATMSX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135074"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2281)
                     /gene="glgB"
                     /locus_tag="VN041_05445"
     CDS             complement(74..2281)
                     /gene="glgB"
                     /locus_tag="VN041_05445"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWU28523.1"
                     /translation="MSYVEDVSVSEQWEKAADGAHHNPHMLLGAHPSTDAAGRTITII
                     RTRRPLAESVTAVFLDGDRLPLEHVARGIWEGRHAGPPSAYRIATVYAGQNEVIAGDP
                     YRHLSALGEMDLHLIAEGRHERLWQVLGAHPRVFDGETGVDFAVWAPDAKAVRVCGDW
                     NSWNGTGHAMRSMGASGVWELFIPDVSVGSRYKYEILTPDDRWILKADPMAQAAEVPP
                     ATASVVAQSAYTWADGAWMTRRAATHAVAQPMSVYELHLGSWREGLGYREIADELIEH
                     VGATGFTHVELMPVAEHPFGGSWGYQVSGYYAPTSRYGSPDDLRYLIDRLHQAGIGVI
                     LDWVPGHFPKDEFALARFDGRPLYEHPDPRRGEHQDWGTLIFDYGRNEVRNFLVANAL
                     YWLSEFHVDGLRVDAVASMLYLDYSRKDGEWEPNVHGGRENLEAIRFLQEVNATAYRL
                     HPGIVMIAEESTAFPGVTAPTSAAGLGFGFKWNMGWMNDSLQYMKRDPMYRSHHEGEI
                     TFSFVYSFGENYVLPISHDEVVHGKGTLVSRMPGDHHHKLANVRAYLGYMWGHPGKKL
                     LFMGQEFGQIGEWSVDRELDWWLLDQPAHAQLQHFVSAMNAVYRRQAPLWERDNDSKA
                     FHRLGAPSWDPSVVAFERHDAHGGRLVVVTNFAGVTRTGYRLQLSCEGTWIEVLNTDA
                     AEYGGSDAGNLGMIHAHCETDDAPAIATVTLPALSTIWFRYQADPHIPTVSHD"
     gene            complement(2278..>3846)
                     /locus_tag="VN041_05450"
     CDS             complement(2278..>3846)
                     /locus_tag="VN041_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWU28524.1"
                     /translation="RLRAAAKRLKGADAGELTAVSTDPALAAVFADRPMASLGSVTDW
                     LELTVERARAGVGSWYEFFPRSEGAVRRKDGTVKSGTFRSAIKRLPGVAGMGFDVLYL
                     VPVHPIGETNRKGRNNTLVTEPGDPGSPYAIGSAAGGHDALHPELGDEKDFAALLRAA
                     RKHGLEVALDLALQASPDHPWVAEHPEWFTTLPDGTIAYAENPPKKYQDIYPLNFDND
                     PEGIYAEMLRVVMHWVGLGVRIFRVDNPHTKPLQFWEWLIARVNAVSPGVVFLAEAFT
                     RPAVMRALAAVGFQQSYSYFTWRNTKAELEEFLTSVSQETSDYMRPNLFVNTHDILTE
                     YLQYGGRAAYRIRACIAATAAPTWGVYAGYELIENVARPGSEENIDNEKYEFKFRDWD
                     GAEQRGESLAPLLRRLNAIRAEHPALGQLRNISFHWSDDDAVLVYSKHLDAAFTGTGR
                     DDTLIVVANLDPHSVRETTVHLDTRIWGVQPGEGFEVEDLLTGAVWSWNDHNYVRLDA
                     FAEPVHILRVKERS"
BASE COUNT          586 a         1287 c         1343 g          630 t
ORIGIN      
        1 ggatccgcgc gtcgccgccg cgcggacccg cctggcgtcg ctgctgttct gaggccgggc
       61 aggccgcgag cggtcagtcg tggctgaccg tcgggatgtg cgggtcggcc tggtagcgga
      121 accagatcgt cgacagcgcg ggtagcgtga ccgtcgcgat cgccggtgcg tcgtcggtct
      181 cgcagtgcgc atggatcatc ccgagattgc ccgcatccga gccgccgtac tcggcggcat
      241 ccgtgttgag cacctcgatc caggttccct cgcaggagag ctgcagccgg taccccgtgc
      301 gcgtcacgcc cgcgaagttt gtcaccacca ccaggcgtcc gccgtgcgcg tcgtgccgct
      361 cgaacgccac cacgctgggg tcccagctcg gtgcgccgag acggtggaac gccttgctgt
      421 cgttgtcgcg ctcccacagc ggcgcctgac gacggtacac cgcgttcatg gcgctgacga
      481 agtgctgcag ctgggcgtgc gcgggctggt cgagcagcca ccagtcgagc tcgcgatcga
      541 ctgaccattc gccgatctga ccgaactcct ggcccatgaa cagcagcttc ttgccgggat
      601 ggccccacat gtagccgagg tacgcgcgca cgttcgcgag cttgtggtgg tgatcgccgg
      661 gcatccggga cacgagcgtg cccttgccgt gcacgacctc gtcgtggctg atgggcagca
      721 cgtagttctc accgaagctg tacacgaacg agaacgtgat ctcgccctcg tggtgcgagc
      781 ggtacatcgg gtcccgcttc atatactgca gcgagtcgtt catccagccc atgttccact
      841 tgaacccgaa accgagcccg gccgcggacg tgggcgcggt cacgccgggg aaggccgtgg
      901 actcctcggc gatcatcacg atgccggggt gcagtcggta ggccgtggcg ttgacctcct
      961 gcaggaagcg gatcgcctcg aggttctcac gcccgccgtg cacgttcggc tcccactcgc
     1021 cgtccttgcg ggagtagtcg aggtacagca tcgaggcgac cgcgtccacg cgcaggccgt
     1081 ccacgtggaa ctcgctgagc cagtacagcg cattcgcgac caggaagttg cgcacctcgt
     1141 tgcggccgta gtcgaagatt agagttcccc agtcctggtg ctcgccgcgg cgcggatccg
     1201 ggtgctcgta cagcggccgg ccgtcgaacc tcgccagggc gaactcgtcc ttcgggaagt
     1261 ggccgggcac ccagtccagg atcacgccga tgccggcctg atgcagccga tcgatcaggt
     1321 accgcaggtc gtccggcgag ccgtagcggc tcgtcggcgc gtagtagccg ctgacctggt
     1381 agccccacga accgccgaag gggtgctcgg cgacgggcat cagctccaca tgcgtgaagc
     1441 cggtcgcgcc gacgtgctcg atgagctcgt cggcgatctc gcggtatccg agaccttcgc
     1501 gccaggagcc cagatgcagc tcgtacacgg acatcggctg tgcgaccgcg tgcgtcgccg
     1561 cacgacgggt catccacgcg ccgtccgccc aggtgtaggc gctctgggcc acgacggaag
     1621 ccgtggcggg cggcacctcg gcggcctgcg ccatcgggtc agccttgagg atccagcggt
     1681 cgtccggagt gaggatctcg tacttgtagc gactgcccac ggagacatcc gggatgaaca
     1741 gctcccacac gccgctggcg cccatcgacc gcatcgcgtg accggtgccg ttccaggagt
     1801 tccagtcccc gcacacgcgc acggccttgg catccggggc ccacacggcg aagtccacgc
     1861 cggtctcgcc gtcgaacacg cgcggatgcg cacccagcac ctgccagagt cgctcgtgcc
     1921 tgccctccgc gatcaggtgc agatccatct cgcccagtgc ggacagatgg cggtaggggt
     1981 cgccggcgat gacctcgttc tgacccgcgt acacggtggc gatgcggtag gcggacggag
     2041 gaccagcatg acggccctcc cagatgccgc gtgcgacatg ctcgagcggg agccggtcgc
     2101 cgtcgaggaa gacggcggtg acggactccg cgagcggtct gcgcgtgcgg atgatcgtga
     2161 tggtgcgacc ggccgcatcc gtgctcggat gcgcgccgag gagcatgtgg gggttgtggt
     2221 gcgcgccgtc ggcggccttc tcccactgct cggatacgga gacgtcctcg acgtagctca
     2281 tgatcgctcc ttcaccctca ggatgtgcac cggctcggcg aaggcgtcga gccgcacgta
     2341 gttgtggtcg ttccagctcc acacagctcc tgtgaggaga tcctccacct cgaacccttc
     2401 accgggctga acgccccaga tgcgcgtgtc gaggtgcacg gtcgtctcgc ggacggagtg
     2461 cgggtccagg ttggcgacga cgatcagtgt gtcatcgcgg ccggtgccgg tgaacgcggc
     2521 atccagatgc ttggagtaca ccagcaccgc gtcgtcgtcg ctccagtgga aggagatgtt
     2581 gcgcagctgc ccgagggcgg gatgctcggc gcggatcgcg ttcagccggc gcagcagcgg
     2641 cgccagcgac tcgccccgct gctcggcgcc gtcccagtcg cggaacttga actcgtactt
     2701 ctcgttgtcg atgttctcct cggagcccgg ccgggcgacg ttctcgatga gctcgtagcc
     2761 ggcgtagacg ccccaggtgg gagccgcggt cgcggcgatg caggcgcgga tgcgatacgc
     2821 cgcgcgcccg ccgtactgca ggtactcagt gaggatgtcg tgggtgttca cgaacaggtt
     2881 gggccgcatg tagtcgctgg tctcctgcga cacgctggtg aggaactcct cgagctcggc
     2941 cttggtgttg cgccaggtga agtacgagta gctctgctgg aagcccacgg cggcgagcgc
     3001 gcgcatgacc gccgggcggg tgaatgcctc ggcgaggaac acgacgccgg gggagacggc
     3061 gttcacgcgc gcgatcagcc actcccagaa ctgcagcggc ttggtgtgcg ggttgtcgac
     3121 acggaagatc cgcacgccga gaccgaccca gtgcatgacg acccgcagca tctccgcgta
     3181 gatgccctcg ggatcgttgt cgaagttcag cggatagatg tcctggtact tcttcggagg
     3241 gttctcggcg tacgcgatgg tgccgtcggg gagcgtggtg aaccactccg gatgctcggc
     3301 gacccacggg tggtcgggag atgcctgcag cgcgaggtcg agggcgacct cgaggccgtg
     3361 cttgcgggcc gcgcgcagca gtgccgcgaa atccttctcg tcgccgagct cgggatgcag
     3421 ggcgtcgtgc ccgcccgcgg cggatccgat ggcgtacggc gagcctggat cacccggctc
     3481 tgtcacgagg gtgttgttgc gccccttgcg gttggtctcg ccgatcggat gcacgggcac
     3541 caggtacagc acgtcgaagc ccatccccgc gacgccgggc aggcgcttga tcgcagaacg
     3601 gaacgtgccg ctcttgaccg tgccgtcctt gcgccggacg gcgccctcgg agcgcgggaa
     3661 gaactcgtac caggagccga cgcccgcacg ggcgcgctcg acggtcagct cgagccagtc
     3721 ggtcacagag ccgagggatg ccatcggacg gtccgcgaag acggcggcga gcgcggggtc
     3781 cgtcgacacc gcggtcagct caccggcatc cgctcccttc agccgcttcg cggcggcgcg
     3841 cagccg
//
LOCUS       DATMSX010000178         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_21278,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000178 DATMSX010000000
VERSION     DATMSX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21278"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VN041_05455"
     CDS             complement(<1..1248)
                     /locus_tag="VN041_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU28525.1"
                     /translation="MHLKSLTLKGFKSFAQPTSFVFEPGVTCIVGPNGSGKSNVVDAL
                     AWVMGEQGAKTLRGGKMEDVIFAGTSTRGPLGRAEVQLTIDNGDGALPIDYAEVTISR
                     TLFRNGSSEYAINGSSCRLLDVQELLSDSGLGREMHVIVGQGRLDTVLQASPEDRRGF
                     IEEAAGILKHRRRKEKTLRKLDAMETNLTRLSDLAGEIRRQLKPLGRQAEIAREAAGI
                     AAIVRDAKARLFADDVVALRTALADHTRTEQERHTERIVLSDQLDTVRTGIQRLEAQQ
                     NSAAVDEARRIAFGLEQAQERMRSLFTLANQRLALLGGEDDTITAITVTQDTIDEAKE
                     EITRISEGLGDAQDAAAAASREVVNARAELDTLDVDIAEQSALVSQYDMRLTTLRGNA
                     DAAASALAAVRGAVLRQENALEAA"
     gene            1804..2082
                     /locus_tag="VN041_05460"
     CDS             1804..2082
                     /locus_tag="VN041_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28526.1"
                     /translation="MSTSLLTDRQHTTSIHPPDTEDRQILNIPDQAALQRLPLSDRLS
                     LRIGLWLLERGLRTGDTVDDASRPSRTERITEHEAITLLTFDLQRGLR"
     gene            2079..3206
                     /locus_tag="VN041_05465"
     CDS             2079..3206
                     /locus_tag="VN041_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28527.1"
                     /translation="MSIATENTENTPIALPAGATLRPLELPARADAGPTAVIREYAEV
                     RNRSILEMTGREDDFLTPEALLTALRSTAQRTKRQWYVERDGAVIGCAALDMSLDGAG
                     DTAIMTVAVPKAAAGQGIGRAVYEHVESAARALGVTRLQHWAEHHDDTGALPVLEAPT
                     GFGSIPADRSALFLERSGFTLEQVERASALVWSPETEGHLRALHATAQQHASDYRIVS
                     WGLPTPVEHRDGYAWLKSRMSTDAPDADLGTPEEIWDAARVVEQDERILRRGWSALVT
                     AAQHVGTGELCAFNELSIAADQTTTSTHQWDTLVLSEHRGHRLGMLVKTAGLLAWRER
                     YPESPRVVTYNAEENRPMLSINEEIGFSPIAYEGAWKKELA"
     gene            3203..4288
                     /locus_tag="VN041_05470"
     CDS             3203..4288
                     /locus_tag="VN041_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28528.1"
                     /translation="MTLPTAELTITEVAVPASLDDADAADFIAAVALNNAVCLADSGV
                     DDYERTAAEMLPMWKDRTDHVHRTFIARAGDSIIGAISLYCGVAEPTSAEVDLMVRPE
                     HWSTGVGQALLDHAEQEARLQGRRVLQAWTLHPAVPGSETLAPKTGWGVISSSPHTRL
                     LEQNGFTLEQVERSSGFDLQADPATVERMLSEAIAAAGPDYRLVEWNLPTPPEQRDGY
                     AWALSRMSTDAPSGDLEIDEETWDAARIARRDARIIGGGQTMSVAAVEHVPTGTLAAF
                     NELVIGEDPTGVTHQYCTLVLKEHRGHRLGQLVKCANVMRWRGIAPASPRISTFNAEE
                     NRPMLDINEAMGFVPRAYAGAWQKRLD"
     gene            4370..5299
                     /locus_tag="VN041_05475"
     CDS             4370..5299
                     /locus_tag="VN041_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28529.1"
                     /translation="MNTDLADAAVTLPPRPRRSRRGSVIPVLASVAVIIASGVATATG
                     PGAEYAQIFPPAWLGISGAAAVLLGVLLRARALRTGRAAGAEAAAWVGAVLLLAASGG
                     FVLDCFRAFFAVTGIPAGAFSQIDVPGAVARGLALAGAFIAIRLARTIRADTGRASRA
                     TGSAAGRLSLLAIGLMLCVPYPLLKTIWWVQGDDSGFDVGFPTMELVLFAVAAGGVIV
                     LTTKLGDVLPRWLVVLGGWGGSLVLMSMGALMVVGLVAQATHLVPATVDLGGGARAVI
                     VFAVYSTWLLLGVAVAAATVLYEERRALVSPAV"
     gene            5311..>5633
                     /locus_tag="VN041_05480"
     CDS             5311..>5633
                     /locus_tag="VN041_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28530.1"
                     /translation="MRRWSRILIVAVPALLLLPATALIAPVSLPLPIGIAVTTCAAAA
                     HATLLLVHRAPTLTLLLITALIAAQAVLTAFFVLLPSSALILLALHAAAARGSRRVAI
                     AVAALA"
BASE COUNT          808 a         2038 c         1897 g          890 t
ORIGIN      
        1 cgcggcttcg agggcgttct cctgccgcag cactgcgccg cgcaccgcgg cgagggcgga
       61 tgccgcggcg tccgcattgc cgcgcagggt cgtcagccgc atgtcgtact gcgacacgag
      121 tgcgctctgc tcggcgatgt cgacatcgag cgtgtcgagc tcggctctcg cgttgacgac
      181 ctcgcgacta gcggcggcag cggcgtcctg agcgtcgccc agtccctcgg agatccgggt
      241 gatctcctcc ttcgcctcgt cgatcgtgtc ctgcgtcacg gtgatggccg tgatcgtgtc
      301 gtcctcgccg ccgagcagcg cgaggcgctg gttcgcgagt gtgaacaggc tccgcatccg
      361 ctcctgcgcc tgctccagcc cgaatgcgat gcggcgggcc tcgtcgacgg ccgcggagtt
      421 ctgctgggcc tcgagccgct ggatgccggt gcgcaccgtg tcgagctggt ccgagagcac
      481 gatccgctcg gtgtgccgct cctgctcggt gcgagtgtga tccgccagcg cggtgcgcag
      541 cgcgaccacg tcgtcggcga acaggcgcgc cttcgcatcg cgaacgatcg ccgcgatccc
      601 ggccgcctcg cgggcgatct cggcctgcct gccgagcggc ttgagctgcc tgcggatctc
      661 ccccgccaga tcgctcagcc gcgtcaggtt cgtctccatg gcgtcgagct tgcggagggt
      721 cttctccttg cgccgacggt gcttgaggat gccggcggcc tcctcgatga atccgcgacg
      781 gtcttcgggc gacgcctgca gcacggtgtc gagccggccc tggccgacga tcacgtgcat
      841 ctctcgtccg agcccggagt cgctgagcag ctcctgcacg tccagcagtc ggcagctcga
      901 gccgttgatc gcgtactcgc tggagccgtt gcggaacagc gtgcggctga tcgtgacctc
      961 ggcgtagtcg atcgggaggg cgccgtcgcc gttgtcgatc gtgagctgca cctcggctcg
     1021 gccgagcggg ccgcgggtcg acgtcccggc gaagatgacg tcctccatct tgccgccgcg
     1081 gagcgtcttc gccccctgct cgcccatcac ccacgcgagc gcgtccacga cgttcgactt
     1141 gcccgagccg ttgggaccga cgatgcaggt cacaccgggt tcgaacacga aggacgtggg
     1201 ctgcgcgaag gacttgaacc ccttgagcgt caggctcttc agatgcatgc cgggactccg
     1261 agcgtcggaa gggcgtcatc ggtcgcggga agcacgccca tacgctaccc gaaagccgcg
     1321 gaacggcggg gatcccgcgc tgcgtcgggt gcgatcccga gaggttcacc gatggcgggc
     1381 ctgaggcgcg acacgccgag gacacgcccg gatgcttgac tccgctggga acttcccgct
     1441 agcgtgactc accgatcaca agagaggagg tcaccgatga tgatcacgaa gctgaacggc
     1501 gtcacttgca ccgccggcac tgtgtcggcg aagtcgccac gtatctcggt ggccttcgca
     1561 cgcacggccg cataacgccg caccgcacct ctcacgactc gtcagacgcc ccgcgtccgg
     1621 tcgatgatcg tcgccccgcg caccgcgcgg ctggcgcccc gcccgttcca gggcgattcc
     1681 cggcacgtcc gctctgcttc tgagcgcgtg ccctccggat gccaccggca tcgcatccgc
     1741 gcgcctcacc ggcatccgct tcgttcccca ccatgagaac aaacgacaga aagcgcacac
     1801 atcgtgagca cctccttgct gaccgaccgt cagcacacca cgtccatcca cccgccggat
     1861 accgaggatc gtcagatcct caacatcccc gaccaggcgg ccctgcagcg gctcccgctg
     1921 tctgaccgcc tcagcctccg catcgggctc tggctgctcg agcgcggcct gcgcaccggc
     1981 gacaccgtcg atgacgcttc gcgcccgagc cgcaccgagc gcatcaccga gcacgaggcg
     2041 atcaccctgc tcaccttcga tctgcagagg ggcctgcgat gagcatcgcg accgagaaca
     2101 ccgagaacac gcccatcgca ctccccgcag gcgcgaccct gcgtccgctc gagctcccgg
     2161 ctcgagcgga cgcggggccg accgccgtca tccgcgagta cgcggaggtc cgcaaccgat
     2221 ccatcctcga gatgaccggt cgcgaggacg acttcctcac gcccgaagct ctcctgaccg
     2281 ccctgcgcag caccgctcag cgcaccaagc gccagtggta cgtcgagcgc gacggcgccg
     2341 tcatcggatg cgcggcgctc gatatgagcc tcgacggcgc gggcgacacc gcgatcatga
     2401 cggtcgccgt tccgaaggcc gccgccggcc agggcatcgg tcgtgccgtg tacgagcacg
     2461 tggagtccgc ggcgcgtgcg ttgggcgtga ccaggctgca gcactgggcc gagcaccatg
     2521 acgacacggg agcattgccc gtactcgaag ctcccaccgg cttcggctcg atccccgccg
     2581 atcgctcagc gctgttcctc gagcgcagcg ggttcacgct cgagcaggtc gagcgggcca
     2641 gcgcgctggt gtggtcgccc gagacggagg ggcatctccg cgccctgcac gccaccgctc
     2701 agcagcacgc gtctgactat cggatcgtgt cgtggggtct gccgacgccc gtcgaacacc
     2761 gcgacggcta cgcctggttg aagtcgcgga tgtcgaccga cgcgccggat gccgacctgg
     2821 gcacaccgga ggagatctgg gacgccgccc gcgtggtcga gcaggacgaa cgaatcctcc
     2881 ggcgcgggtg gtcggcgctg gtcacggccg cgcagcacgt cggcaccggc gagctgtgcg
     2941 ccttcaacga gctgtcgatc gccgcggatc agacgacgac gagcacgcat cagtgggaca
     3001 cgctcgtgct ctccgagcat cgtggtcatc ggctcgggat gctcgtgaag accgcgggcc
     3061 tgctcgcctg gcgcgagcgc taccccgagt caccccgtgt cgtcacctac aacgccgagg
     3121 agaaccggcc gatgctctcg atcaacgagg agatcggctt ctcccccatc gcctacgaag
     3181 gcgcctggaa gaaggaactc gcatgaccct ccccaccgca gagctgacga tcaccgaggt
     3241 cgccgtcccc gcgtcgctgg acgatgcgga tgctgccgac ttcatcgcag ccgtggccct
     3301 gaacaacgcc gtgtgcctgg cagacagcgg cgtggacgac tacgagcgca ccgccgccga
     3361 gatgctgccc atgtggaagg accgcaccga ccacgtgcac cgcaccttca tcgcgcgcgc
     3421 cggcgacagc atcatcggtg cgatctcgct gtactgcggg gtcgctgagc ccaccagcgc
     3481 agaggtcgac ctgatggtgc gcccggagca ctggtccacg ggcgtcgggc aggctctgct
     3541 cgaccacgcg gagcaggagg cgcggctgca gggtcggcgg gtcctgcagg cgtggaccct
     3601 gcacccggcc gtccccggca gcgagaccct cgccccgaag accggctggg gcgtgatctc
     3661 gtccagcccg cacacccgcc tgctggagca gaacggcttc accctcgagc aggtggagcg
     3721 cagcagcggc ttcgatctgc aggccgatcc agcgacggtc gagcggatgc tgtcagaggc
     3781 gatcgccgcg gcggggccgg actaccgtct cgtcgagtgg aacctgccga ccccgcccga
     3841 gcagcgcgac ggctacgcct gggcgctgtc gcggatgtcg acggacgctc ccagcggaga
     3901 cctcgagatc gacgaggaga cctgggacgc ggcccgcatc gcccgacgcg acgcgcgcat
     3961 catcgggggc ggtcagacga tgtcggtcgc cgcggtggag cacgtgccaa ccggcacgct
     4021 ggccgccttc aacgagctgg tcatcggcga ggaccccacc ggtgtgaccc atcagtactg
     4081 cacgctggtg ctgaaggagc accgcgggca tcgtctcggt cagctcgtga agtgcgcgaa
     4141 cgtcatgcgc tggcgcggca tcgcgccggc gtcgccgagg atctcgacgt tcaacgccga
     4201 ggagaaccgg ccgatgctcg acatcaacga ggcgatggga ttcgtgccgc gggcgtacgc
     4261 cggcgcctgg cagaagcgcc tggactgagc aggtggatcg atactcgggt cgctcacgca
     4321 gcgacccgag tacgatccgc cgccgcccgc ggtggcgaac gctggagtca tgaacaccga
     4381 cctcgcagac gcagccgtca cgctcccacc ccgaccgcgc cgctcccggc gcgggtccgt
     4441 cattcctgtc ctcgcctctg tcgcggtgat catcgcatcc ggtgtcgcca ccgccaccgg
     4501 tcctggtgcg gagtacgcgc agatcttccc accggcctgg ttggggatct cgggcgctgc
     4561 ggccgttctc ctcggagtgc tgctgcgcgc tcgcgcactg cgcaccggac gcgccgcggg
     4621 tgccgaagcg gcggcctggg tcggcgccgt gctgctgctc gcagcatccg gcgggttcgt
     4681 gctcgactgc ttccgggcgt tcttcgccgt gaccggcatc ccggccggtg ccttctccca
     4741 gatcgacgtc cccggtgctg tcgcgcgcgg tctcgccctg gcgggcgcgt tcatcgccat
     4801 ccggctcgcc cggaccatac gcgccgacac gggacgcgcg tcgcgtgcaa ccggatcggc
     4861 cgcaggccgg ctctcgctgc tggccatcgg actcatgctg tgcgtcccct atccactgct
     4921 caagacgatc tggtgggtgc agggcgacga cagcggcttc gatgtgggat tcccgacgat
     4981 ggagctcgtc ctgttcgccg tcgcggcagg gggcgtcatc gtgctcacga cgaagctcgg
     5041 agacgtcctg ccccgatggc tggtcgtgct gggcggctgg ggcggatccc tcgtgctcat
     5101 gagcatgggc gccctgatgg tcgtcggcct cgtcgcccag gcgactcacc tcgttcctgc
     5161 gacggtggac ctcggcggtg gggcgcgagc cgtgatcgtg ttcgcggtgt actcgacctg
     5221 gttgctgctc ggtgttgccg tcgctgcggc tacggtgctg tatgaggagc ggcgggcact
     5281 cgtctcgccc gcggtgtgag aggagtccgg atgcgtcgat ggagccggat cctgatcgtc
     5341 gccgtgccgg cgctgctcct gctgccggct acggcgctca tcgcgcccgt ctcgctgccg
     5401 ctcccgatcg gcatcgcggt gacgacctgt gcggcggcgg cacacgcgac cctgctcctg
     5461 gtgcatcggg caccgaccct cacgctgctg ctcatcacgg cactcatcgc cgcacaggcc
     5521 gtgctcacgg cgttcttcgt gctcctgcca tcctctgcgc tgatcctgct cgccctgcat
     5581 gcggcggcgg cgcgcgggag cagacgagtc gcgatcgccg tcgccgcgct cgc
//
LOCUS       DATMSX010000179         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_59611,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000179 DATMSX010000000
VERSION     DATMSX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59611"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VN041_05485"
     CDS             complement(<1..337)
                     /locus_tag="VN041_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWU28531.1"
                     /translation="MSTALSPLHEALTVLDDAWADAGEVAELSRSELMAANAALGALK
                     RRVDALAVQVAAGIAHESRAELGAESLAKQAGFRTPAALIAATTGVSTGDAVRLVRVG
                     EAVAPRVDLV"
     gene            complement(447..1079)
                     /locus_tag="VN041_05490"
     CDS             complement(447..1079)
                     /locus_tag="VN041_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28532.1"
                     /translation="MSQRRSRATMLRRRARRRRERKRMLRIGAITVSVALALSAVFVG
                     VGLLTAPQGERYCAERPQTFPDRGVAGWSGDQLMNAAIIVSTARTLGFGRDGQVLGVM
                     AAMGESSLHNIGYGDWETSGVRNPDGSRTSSIGLFQQQKWWGSDEERMDPATAATKFY
                     VRLGKIAGWQQLPPSHAIHRVQINADQEHYARFQEDATAVVDALSGPCPG"
     gene            complement(1076..1675)
                     /locus_tag="VN041_05495"
     CDS             complement(1076..1675)
                     /locus_tag="VN041_05495"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWU28533.1"
                     /translation="MTASAPPVARSPKATRFLAPLGLLALMWAIQFADAVLPGTFVGW
                     GLRSWDLGSLQGLVLGPLLHASWTHLIGNSVPFLILGCLVAVEGARRFWLVTAMVALV
                     GGAGTWLLNAPGTLTVGASVLVFGYFGYVVLRVIAPGRLAHRIAYAGIAVIVIVLYGT
                     TVLTGIFGAGPGVSWQAHLFGAIGGGLVAFIGRRRDPAA"
     gene            1761..2648
                     /locus_tag="VN041_05500"
     CDS             1761..2648
                     /locus_tag="VN041_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWU28534.1"
                     /translation="MGSLTRDKRIALLDAPSNLGLRPPVDGVAPGCYKAPWALRDAGL
                     LHRLGAHDAGAVVPPRYDSTWSPGAGVRNGRVIATYAGRLASRIGGILDAGERPLVLG
                     GDCSILLGPALALRRRGRFGLAFIDAHDDFRHLGNSPHVGAAAGEELALVTGRGEDYL
                     TDIDGLRPYFREEDIVAIGVRDPEIFADFTESDIALVSGVDGPADAARDVLVRDELDG
                     FWIHVDADILDPSVLPAVDSPEPGGLSPDQLIDLLAGLLALPGAIGMELTVFDPDLDE
                     DGAQAALLTDILVEAASRR"
     gene            complement(2723..2819)
                     /gene="ffs"
                     /locus_tag="VN041_05505"
     ncRNA           complement(2723..2819)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN041_05505"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2913..>4594
                     /locus_tag="VN041_05510"
     CDS             2913..>4594
                     /locus_tag="VN041_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWU28535.1"
                     /translation="MTPQPGPLADWRYDGELRTYQSEVLAQIPASPADAAMHIVAPPG
                     SGKTLLGLLLAVREQRRALVLAPTVTIREQWVRTARSLTTDEARVGDDPTVLADLTVL
                     TYQLLSVTGDSSPFDDLARAQWADELAAAGRTEADAATWLATIAIDNRAQYLRGIRKR
                     AASLRRRFAQARPDLLARVLHPNALALIERIVDAGVDTIVLDECHHLLDHWAIVVAYL
                     RARIRERGGSGLLIGLTATLPSPDDGTGFENYTGLLGDVDYEVPTPAVVKEGHLAPYR
                     SYVRFTEPVPQEAHFIRRSEAQLHELMVQVLASPDGIAYLERQLQPAAPVQDAEADAD
                     QHPLPPEEIARRGIDRALSDDFPLTRTCGAVLRAIAPEHPLVAFLDPQLFGRASTDGL
                     LLVLSRFALARLLPDPAARDQWRYVKGALADFGLHLTDRGIRRGRNPLEATLSTSVGK
                     DHAAVDILRDELRSPDGGRIRAVVVTDVVEAGDNRGLTGDAAPGALRVFDLLASEPET
                     AALTPVLLTAQHLRTTAADAERIASALSPMLDTAVEIEDGFGPTRVLRVPGF"
BASE COUNT          715 a         1721 c         1508 g          650 t
ORIGIN      
        1 cgaccagatc cacccggggc gccaccgcct ccccgaccct caccagtcgg acagcatccc
       61 ccgtcgaaac ccccgtcgtc gcggcgatca gagccgcggg agtccggaac ccggcctgct
      121 tcgccagcga ctccgccccc agctccgcac gcgactcatg cgcgataccc gccgcgacct
      181 gcacggccag cgcatcgacg cgtcgcttca gagccccgag cgccgcgttc gcggccatca
      241 gttccgagcg cgacagctcg gccacctctc cagcatccgc ccatgcgtca tcgaggaccg
      301 tgagggcctc atgcaggggc gacagtgccg tggacatacc accagcctac gaacgatcac
      361 ccatactgtc aagcatttat tctaaagaca gacacgctct tgcgcacgac tttagaacat
      421 acatcccata tcagcgactg ccgacattac cccggacacg gccccgacag ggcatccacc
      481 actgccgtcg cgtcctcctg gaagcgcgcg tagtgctcct ggtcggcgtt gatctgcacc
      541 cggtggatgg cgtgcgaagg cggcagctgc tgccagcccg cgatcttccc cagacgcacg
      601 tagaacttcg tcgcggcggt ggcgggatcc atgcgctcct cgtcgctccc ccaccacttc
      661 tgctgctgga acagcccgat gctggacgtg cggctgccgt cggggttcct gacgcccgag
      721 gtctcccagt ccccgtagcc gatgttgtgc aacgagctct cccccatcgc cgccatgacg
      781 ccgagcacct ggccgtcgcg cccgaagccc agagttcgcg cggtgctcac gatgatcgcg
      841 gcgttcatga gctggtcgcc gctccacccg gccacgcccc gatcggggaa cgtctggggc
      901 cgctctgcgc agtaccgctc accctgcggg gcggtgagca gccccacccc cacgaacacg
      961 gcggagagag ccagagcgac ggacaccgtg atcgctccga tccgcagcat ccgcttcctc
     1021 tcgcggcgac gacgagcgcg gcgccgcagc atggtggcgc gggatcggcg ctgactcacg
     1081 cggccgggtc cctccgcctg ccgatgaagg ccaccagccc gccgccgatc gcaccgaaga
     1141 ggtgcgcctg ccaggagacg ccgggaccgg caccgaagat gccggtcagc accgtggtgc
     1201 cgtagagcac gatgacgatc accgcgatgc cggcgtacgc gatgcggtgc gcgaggcgcc
     1261 ccggcgcgat gacgcgcagc acgacgtacc cgaagtagcc gaacaccagc acggatgctc
     1321 cgaccgtgag cgtccccggt gcgttgagca gccaggtccc ggcgcctccg accagtgcga
     1381 ccatcgccgt cacgagccag aaccgcctcg caccctcgac ggcgacgagg catccgagga
     1441 tgaggaaggg cacggagttg ccgatcaggt gcgtccagct cgcgtgcagc agcggaccca
     1501 gcacgaggcc ctgcaggctg ccgagatccc acgaccgcag cccccagccg acgaaggtgc
     1561 ccggcagcac ggcatccgcg aactggatcg cccacatcag cgcgagcagg ccgagcggcg
     1621 cgaggaagcg cgtcgccttc ggtgagcgcg cgacgggagg agcggatgct gtcacacgac
     1681 gatcctcgca ggtgcgctgg ggagtggact gagcgcgcac gccgacgcgt gtgtccgtcc
     1741 ccgcccgtaa catcacgctc atgggctctt tgactcgtga caagaggatc gcactgctcg
     1801 atgcgccatc gaacctcggc ctgcgaccac ccgtggacgg cgtcgccccg gggtgctaca
     1861 aggcgccgtg ggcattgcgc gacgccggac tgctccatcg cctcggcgcc cacgatgccg
     1921 gcgccgtggt cccgccgcgc tacgactcga cgtggtcgcc gggggccggc gtgcgcaacg
     1981 gcagagtcat cgcgacgtac gcgggcagac tcgcctcgcg catcggcgga atactggatg
     2041 ccggcgagcg gccgctcgtg ctcggaggcg actgctcgat cctgctcggc cctgctctgg
     2101 cgctgcgccg gagaggacgt ttcggtctgg ccttcatcga cgctcacgac gacttccggc
     2161 acctcggcaa ctcaccgcac gtcggcgcgg cggctggaga agaactggca ctcgtcaccg
     2221 ggcgaggcga ggactacctg accgacatcg acggcctgcg cccgtatttc cgggaagagg
     2281 acatcgtcgc catcggtgtt cgtgatcccg agatcttcgc ggacttcacc gaaagcgata
     2341 tcgctctcgt ctcgggggtg gacgggccgg cggatgctgc gcgcgacgtc ctcgtcagag
     2401 acgagctcga cgggttctgg atccacgtgg atgccgacat cctcgacccg tccgtcctgc
     2461 cggccgtcga ctcccccgag cccggcggac tcagcccaga tcagctcatc gacctgctcg
     2521 caggcctgct cgcgttgccc ggagcgatcg gcatggagtt gaccgtgttc gatcctgacc
     2581 tcgacgagga cggggctcag gcggcccttc tcacggacat cctcgtggaa gcggcgagcc
     2641 ggcgctgaac ccgcaacgac ggcgttcgag cgcctctgac agcgggcttc tgcgcggcac
     2701 ggtgcacgga catcagggca aaagaaaaga ccctccgcgc acccgacaga gcccggttac
     2761 ccttgctacg tttccgtcct gggggaattg gcctggatgc cgtcacgcgg agagccgtcc
     2821 acgagtctac ccgaccggag gggcgccctt gaccgccggt ccgcgctccg ctcgctcaat
     2881 gaccggggac tccgccgcta cgctcgatca cgatgacacc gcagcccgga ccgctcgccg
     2941 actggcggta cgacggcgag ctgcgcacct accagtccga ggtgctggcg cagataccgg
     3001 cttccccggc ggacgccgcg atgcacatcg tcgcaccgcc cggatccggc aagacactgc
     3061 tcggcctgct gctggcggtg cgcgagcagc gtcgcgcact cgtactcgcc ccgacggtca
     3121 cgatccgaga gcagtgggtg cgcacggcac gctccctcac aacggacgag gcccgcgtcg
     3181 gagacgaccc cacggtgctc gccgacctca cggtgctgac ttatcagctg ctgagcgtca
     3241 cgggcgacag ctcccccttc gacgacctcg cccgggcgca gtgggctgac gagctggccg
     3301 cggcgggccg caccgaggcg gatgccgcga cctggctcgc cacgatcgcg atcgacaacc
     3361 gggcgcagta tctgcgcggg atccgcaagc gcgccgcctc gctccgccgc cgcttcgcgc
     3421 aggcgcggcc cgaccttctc gcccgggtac tgcatccgaa cgcgctcgcc ctgatcgagc
     3481 gcatcgtcga cgccggcgtc gacacgatcg tgctcgacga gtgccaccat cttctggatc
     3541 actgggcgat cgtcgtcgcc tacctgcggg cgcggatccg cgagcgcggc gggagcggcc
     3601 tgctcatcgg gctgaccgcg acgctgccct cccccgacga cggcaccggg ttcgagaact
     3661 acaccggcct gctcggcgat gtcgactacg aggtgccgac cccggcggtc gtgaaggagg
     3721 ggcacctcgc gccgtaccgc tcgtacgtgc gcttcaccga gccggtcccg caggaggcgc
     3781 acttcatccg ccggagcgag gcgcagctgc acgagctcat ggtgcaggtg ctcgcctccc
     3841 ccgacggcat cgcctacctc gaacggcagc tgcagccggc cgcgcccgtg caggatgcgg
     3901 aggcggatgc cgatcagcat ccgctcccgc ccgaggagat cgcccgccgt gggatcgacc
     3961 gcgccctcag cgacgacttc cccctcaccc gcacctgcgg cgccgtgctg agggcgatcg
     4021 cgcccgagca tccgctggtc gcgttcctgg atccgcagct cttcggccgg gcgagcaccg
     4081 acggtctgct gctggtgctc tcccgcttcg ccctcgcgcg gctgctgccc gaccccgccg
     4141 cacgcgacca gtggcgctac gtgaagggcg cgctggccga cttcgggctg catctgacgg
     4201 atcgcggcat ccgccgggga cgcaacccgc tcgaggccac gctgtcgacc tcggtgggga
     4261 aggatcacgc cgcggtcgac atcctgcggg acgagctgcg ctcccccgat ggcgggcgca
     4321 tccgcgcggt ggtcgtcacg gatgtcgtgg aggccggcga caatcgcggc ctcaccgggg
     4381 atgccgcgcc cggcgccctg cgtgtgttcg acctgctggc atccgagccg gagaccgccg
     4441 cgctcacacc ggtgctgctc acggctcagc acctgcgcac gaccgccgcg gatgccgagc
     4501 gcatcgcgag cgcgctgagc ccgatgctcg acaccgcggt cgagatcgag gacggattcg
     4561 gccccacccg ggtgctgcgc gtgccgggct tcag
//
LOCUS       DATMSX010000180         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_116527,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000180 DATMSX010000000
VERSION     DATMSX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116527"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN041_05515"
     CDS             <1..228
                     /locus_tag="VN041_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWU28536.1"
                     /translation="MRAFLLGRGAGESFFAGVDAEAADAAEDLRARTVELPAPGRGAI
                     FDHVYSEPHPLIAEQKAWLEQYEASFEGGAA"
     gene            225..1205
                     /locus_tag="VN041_05520"
     CDS             225..1205
                     /locus_tag="VN041_05520"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWU28537.1"
                     /translation="MTLETMPFSKALNAGLRKALEDDPRVLLMGEDIGKLGGVFRVTE
                     GLQRDFGDRRVLDTPLAESGIVGTAIGLAMAGFRPVCEIQFDGFVFPAFDQITSQLAK
                     MTNRHEGVLSMPIVIRIPYGGHIGAVEHHQESPEAYFTHTPGLRVVSPSTPNDAYWMI
                     QEAIASNDPVIFMEPKSRYWPKGEVDLSGSALPLHASRIVRRGTDVTLVGHGAMVTTL
                     LQAAALAEAEGTSCEVVDVRSLSPVDYGPILDSVRSTGRMVYAQEAPGFTSLGSEIAA
                     TVQERAFYALEAPVLRVSGFDTPFPPAKLEGVYLPDADRILEAVDRSLAY"
     gene            1259..>1593
                     /locus_tag="VN041_05525"
     CDS             1259..>1593
                     /locus_tag="VN041_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWU28538.1"
                     /translation="MTTQTFTLPDVGEGLTEAEIVTWKVAPGDTVAINDVICEIETAK
                     SLVELPSPHAGTVGEILVAEGVTVEVGSPIITFVTDAPDAAPAAAPAAPAADEGSGSV
                     LVGYGSGGGA"
BASE COUNT          243 a          573 c          533 g          244 t
ORIGIN      
        1 atgcgcgcgt tcctgctggg gcgcggcgcc ggcgaatcgt tcttcgccgg ggtggatgcc
       61 gaggccgcgg atgctgccga ggatctgcgc gctcgcaccg tggagctccc cgcgccggga
      121 cgcggggcga tcttcgacca cgtgtacagc gaaccgcatc cactgatcgc cgagcagaag
      181 gcgtggctcg aacagtacga ggcatccttc gagggaggcg cagcatgacc ctggagacaa
      241 tgcccttctc gaaggcgctc aacgccgggc tgcgcaaggc gctcgaggac gacccgcgcg
      301 tgctgctcat gggcgaggac atcggaaagc tcggcggcgt cttccgggtg accgagggtc
      361 tgcagcgcga tttcggcgac cggcgcgtgc tcgacacccc cctcgccgag tccggcatcg
      421 tcggcacggc gatcggcctg gccatggccg gcttccgccc ggtgtgcgag atccagttcg
      481 acggcttcgt cttccccgcc ttcgaccaga tcacctcgca gctcgcgaag atgaccaacc
      541 ggcatgaggg cgtgctgtcg atgccgatcg tcatccgcat cccctacggc gggcacatcg
      601 gcgcggtgga gcaccaccag gagagccccg aggcgtactt cacgcacacg cccggcctgc
      661 gggtggtctc gccctccacg cccaacgacg cctactggat gatccaggag gccatcgcgt
      721 cgaacgaccc ggtgatcttc atggagccca agagccgcta ctggcccaag ggcgaggtgg
      781 atctctccgg ctccgcgctt ccgctgcacg cctcgcggat cgtgcgccgc ggcacggatg
      841 tcaccctcgt cgggcacggt gcgatggtca cgaccctgct gcaggccgca gcgctcgccg
      901 aagccgaggg caccagctgc gaggtcgtcg acgtgcgctc gctctcgccg gtggactacg
      961 gtcccatcct cgactcggtg cgctccaccg gacgcatggt ctacgcgcag gaggcgcccg
     1021 gcttcaccag cctgggcagt gagatcgccg cgaccgtgca ggagcgcgcg ttctacgccc
     1081 tcgaagcccc ggtgctgcgt gtctcgggct tcgacacgcc cttcccgccc gccaagctcg
     1141 agggcgtcta cctgccggat gccgaccgca tcctcgaagc cgtcgaccgc tcgctggcct
     1201 actgaacgct gcctggggca ctgagcctgt cgaagtgccc cagagtacgg aggaaaccat
     1261 gaccactcag accttcaccc ttcccgatgt cggcgagggc ctcaccgagg ccgagatcgt
     1321 cacctggaag gtcgcacccg gcgacaccgt cgcgatcaac gacgtgatct gtgagatcga
     1381 gaccgccaag tcgctcgtcg agctgccctc tccgcacgcc gggacggtcg gggagatcct
     1441 cgtcgccgaa ggcgtcacgg tcgaggtcgg gtcgccgatc atcaccttcg tcacggatgc
     1501 tccggatgcc gcgcctgcgg ctgcgcccgc agcccccgcc gcggatgagg gcagcggctc
     1561 ggtgctcgtc ggctacggca gcgggggagg cgc
//
LOCUS       DATMSX010000181         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135122,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000181 DATMSX010000000
VERSION     DATMSX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135122"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1227
                     /locus_tag="VN041_05530"
     CDS             49..1227
                     /locus_tag="VN041_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU28539.1"
                     /translation="MTPGRPLKILIGADTFAPDINGAARFAERLAAGLVQRGHDVHVV
                     APNTAYRRSATHTEIIEGEPLTMHRLPAVRWLPHDWLRFVWPWRSKHYARKVLDEVQP
                     DVVHIQSHIVIGRGLTRIAHQRGIPVIATNHVMAENILDHTTMPDFLNRPILKLAWDD
                     AKRTFDMTRAVTTPTRKAADFLEHTIEIKGVVPISCGIDRRNYTPVIGPREQNRILFV
                     GRLTGEKKVDVVLHALAKLDPALDAHFDIAGGGDQRKALEALAAQLGLADRVTFHGRV
                     TDEELRELYSRASVFAIASIAELQSIVTMEAMASALPVVGANAVALPHLVHDGENGYL
                     FEPGNVDDLVDKLTKVLTAAPEEYERMQKASLDGVAVHDINRTLDTFEALYRDEPLPE
                     "
     gene            1349..1981
                     /locus_tag="VN041_05535"
     CDS             1349..1981
                     /locus_tag="VN041_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28540.1"
                     /translation="MSEEVQGSSQGSASDAGTPGSQPRAAAAGSARPTFRERLALWAD
                     AFKAIKWKRVLILGGIGIVVAAIAVVLIVNFVPVGWSRFIGDFAQNDAGRAILVGLIV
                     GFCFTLVPLMVLLLLAYRSPAWVKVVLVVVAVLLATPNLLTLWIQTGASGSVHAADRI
                     MDTEAYGFSGASIWGAVIGALAFLVWAWLFISSRLTKKKLKTLRETPAGS"
     gene            complement(1976..2479)
                     /locus_tag="VN041_05540"
     CDS             complement(1976..2479)
                     /locus_tag="VN041_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28541.1"
                     /translation="MSDPQQPQPAQPNQPPYAQPEPAQSPPAQSPYAQPATSQGYPAS
                     AGYPQATGPQPGYGVAPASGPGSLGRTAFIIALVTAALGLLFVIMSPIIIAGMRGQYG
                     LYEVISLVRSVVGLALGVTALILGVIAARRRSQPVLAGIAIGVGGVEALGVVFSLLSN
                     LMYGVLL"
     gene            complement(2545..2667)
                     /locus_tag="VN041_05545"
     CDS             complement(2545..2667)
                     /locus_tag="VN041_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28542.1"
                     /translation="MFIVVILSALALWAVIATIVELRRDGFRRTPTDWTRVRGE"
     gene            2765..3745
                     /locus_tag="VN041_05550"
     CDS             2765..3745
                     /locus_tag="VN041_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28543.1"
                     /translation="MKTVACVIDNGFAPFEFGVACEAFGLDRSDDGVPNFDFRVVAPE
                     PGLVRSKIGFSVEVENDLSFAYEADLVIFCPMPREYWGSMDPRLIDLARDAVARDAWV
                     MSVCSGSFVLAAAGVLDGRRATTHWMYAHVLAEMYPRIDVDPDVLFVQDGRIITSAGT
                     AAGIDACLHLLRIELGAEMAGRIARRMVVPPQRDGGQAQYVDRPLPVVASGSLASVAD
                     WAVQNIRDELSIDQLAGHAHMSARTFARRFKAEFGATPASWLTRQRVIHAQRMLERTD
                     LGLDRIAEECGFGSAAVLRQNFARVLDTTPTAYRARFSCRDERMLEPVPA"
     gene            complement(3946..4635)
                     /locus_tag="VN041_05555"
     CDS             complement(3946..4635)
                     /locus_tag="VN041_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HWU28544.1"
                     /translation="MCTVVIDVADAGARLLAVRDEDPQRAWDDLGEWWPDAYPGVIGI
                     HDRRAGGAWLALDPQTHRLAVLLNRADVLDLPEADVLSRGSLALESVAGRSPEAPLRM
                     HGFNLLEVTPTGSRVLHWDGDTLRETPVRHGVHMIAHDDLDDPRTARIETWLPHFAEA
                     RPDDEGWTGRWVELLARSAELDPDDDRAIIRDNNAHGYPTESLLYCTAEVSADAASVR
                     SHVLAEPGHWD"
     gene            4820..>5287
                     /locus_tag="VN041_05560"
     CDS             4820..>5287
                     /locus_tag="VN041_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWU28545.1"
                     /translation="MNIVVGVTGGIAAYKAVHLVRLLIKDGHDVTVIPTEDALRFVGL
                     PTWEAISRNPVTTSVHEDVAKVRHVALGQAAELVIVAPATANTIAKMTAGLADDLLGT
                     TLLATTAPVAIAPAMHTEMWRHPATQANIDTLRERGVAILGPAEGELTGGDSGP"
BASE COUNT          801 a         1871 c         1846 g          769 t
ORIGIN      
        1 ggcgcacgac gccgcgaccc ctgagctaca gataggcagc atccttcctt gactcccgga
       61 cgtcctctga agatcctcat cggcgccgac accttcgcgc ccgacatcaa cggcgccgcc
      121 cgcttcgcgg agcgcctcgc cgcaggcctc gtccagcgcg gccacgacgt gcacgtcgtc
      181 gcgccgaaca ccgcctaccg tcgctccgcc acccacaccg agatcatcga gggcgagccg
      241 ctgaccatgc accggctgcc cgccgtgcgc tggctgccgc acgactggct ccggttcgtc
      301 tggccgtggc gatccaagca ctacgcccgc aaggtgctcg acgaggtgca gccggacgtc
      361 gtgcacatcc agtcgcacat cgtgatcggc cgcgggctga cccgcatcgc ccaccagcgc
      421 ggcatcccgg tgatcgcgac caaccacgtg atggccgaga acatcctcga ccacaccacc
      481 atgccggact tcctcaaccg gccgatcctc aagctggcct gggatgacgc gaagcgcacc
      541 ttcgacatga cccgggcggt caccacgccc acgcgcaagg ccgccgactt cctcgagcac
      601 acgatcgaga tcaagggcgt ggtccccatc agctgcggca tcgaccgccg caactacacg
      661 ccggtgatcg gaccgcgcga gcagaaccgc atcctgttcg tcgggcgcct gaccggtgag
      721 aagaaggtcg acgtcgtgct gcacgctctc gcgaagctcg accccgcgct cgacgcgcac
      781 ttcgacatcg ccggcggcgg agaccagcgc aaggcgctcg aggcgctcgc cgcccagctg
      841 gggctggccg atcgcgtcac cttccacggc cgcgtcaccg acgaggagct gcgcgagctg
      901 tactcccggg catccgtgtt cgccatcgcc tcgatcgccg agctgcagtc gatcgtgacg
      961 atggaggcca tggcatccgc cctgcccgtg gtcggcgcga acgccgtcgc cctgccgcac
     1021 ctcgtgcacg acggcgagaa cggctacctg ttcgagccgg ggaacgtcga cgacctggtc
     1081 gacaagctca cgaaggtgct cacggccgcg cccgaggaat acgagcggat gcagaaggcc
     1141 tcgctcgacg gcgtggccgt gcacgacatc aaccgcacgc tcgacacctt cgaggcgctg
     1201 taccgggacg agccgcttcc ggagtgagac ggcgccctac gtctctgcga taacggcgcc
     1261 gtacgtctct gcgatgcctg cgccgacgat ggcctgcgcc tacgattggt cgtgccgtcg
     1321 ccgacgacgg cggaaccggg ggatgggcat gtccgaggaa gtgcagggat cgtcgcaggg
     1381 gtcggcgtcg gatgccggaa cgccggggtc gcagccgcgc gccgcggccg ccgggtccgc
     1441 gcggccgacg ttccgtgagc ggctcgcgct gtgggccgac gcgttcaagg ccatcaagtg
     1501 gaagcgcgtg ctgatcctgg gcgggatcgg gatcgtcgtc gcggccatcg ccgtggtgct
     1561 gatcgtgaac ttcgtgccgg tgggctggtc ccgcttcatc ggcgacttcg cccagaacga
     1621 cgccgggcgg gcgatcctgg tgggactgat cgtggggttc tgcttcacac tggtgccgct
     1681 gatggtgctg ctgctgctcg cctaccgatc acctgcgtgg gtgaaggtcg tgctggtcgt
     1741 ggtcgcggtg ctgctggcga cgcccaacct gctgacgctg tggatccaga ccggggcgtc
     1801 cgggtccgtg cacgcggccg accggatcat ggacaccgag gcctacggct tcagcggcgc
     1861 gagcatctgg ggcgccgtga tcggggcgct cgcgttcctc gtgtgggcat ggcttttcat
     1921 ctccagccgg ctgacgaaga agaagctcaa gacgctgcgt gagacgcctg cggggtcata
     1981 gcagtacgcc gtacatcagg ttcgagagca gcgagaacac gactcccagc gcctcgacgc
     2041 cgccgacgcc gatcgcgatg ccggccagta cgggctggga gcggcgtcgc gcggcgatga
     2101 cgcccaggat gagggcggtc actccgagag cgaggcccac caccgagcgg acgagggaga
     2161 tgacctcgta gaggccgtac tgcccgcgca tgccggcgat gatgatcggc gacatgatca
     2221 cgaacagcag gcccagtgcc gccgtgacca gggcgatgat gaacgccgtg cgaccgagcg
     2281 agccggggcc ggatgccggg gcgacgccgt agccgggctg cgggcccgtt gcctgcgggt
     2341 agccggcgga ggcgggatag ccctggctcg tggcgggctg cgcgtagggg gactgcgcgg
     2401 gcggggactg cgcgggctcc ggctgcgcat acggcggctg gttcggctgt gcgggctgag
     2461 gctgctgcgg atcgctcatg cgcaagagac tacggggcgt gcggtcagag gtgtgcgggt
     2521 cttgactccc cgcgcgccgt gcgctcactc tccgcggacg cgggtccagt ccgtcggcgt
     2581 ccggcggaat ccgtcgcggc gcagttcgac gatggtggcg atgacggccc acagggccag
     2641 ggctgagagg atgacgacaa tgaacatggc agaaatgcta tggtcggcgc taaactgtca
     2701 cgagtggcag cggagacagt gagcgtagga ttactgccac atcgcgtgtc gtcgaggagg
     2761 acccatgaag accgtcgcct gcgtgatcga caacggcttc gcgcccttcg agttcggggt
     2821 ggcgtgcgag gcgttcgggc ttgaccgctc ggatgacggt gtgccgaact tcgacttccg
     2881 cgtggtcgct ccggagccgg gcctcgtccg ctccaagatc gggttctccg tcgaggtcga
     2941 gaacgacctc tcgttcgcct acgaggccga cctggtgatc ttctgcccga tgccgaggga
     3001 gtactggggt tccatggacc cccgcctgat cgacctcgcg cgggatgccg tcgcccgcga
     3061 cgcgtgggtg atgagcgtgt gcagcggatc cttcgtgctc gccgccgccg gcgtgctcga
     3121 cgggcgtcgt gcgaccacgc actggatgta cgcgcacgtc ctggccgaga tgtacccgcg
     3181 gatcgacgtg gaccccgacg tgctcttcgt gcaggacggg cgtatcatca ccagcgccgg
     3241 aaccgcggcg ggcatcgacg cctgcctgca cctgctgcgc atcgagctgg gtgcggagat
     3301 ggccgggcgc atcgcccgcc gcatggtggt gcccccgcag cgcgacggcg ggcaggcgca
     3361 gtacgtcgac cgcccgctcc ccgtcgtggc gagtggatcg ctggcatccg tcgccgactg
     3421 ggccgtgcag aacatccgcg acgagctctc catcgatcag ctcgcgggcc acgcgcacat
     3481 gtcggcgcgc accttcgctc gccggttcaa ggcggagttc ggcgcgaccc cggcatcctg
     3541 gctcacccgg cagcgggtga tccacgccca gcggatgctg gagcgcaccg acctcggact
     3601 ggaccgcatc gccgaggagt gcggcttcgg gtccgccgcc gtgctgcggc agaacttcgc
     3661 ccgcgtgctc gacaccacgc ccaccgcgta ccgcgcgcgg ttctcctgcc gcgacgagcg
     3721 gatgctcgag cccgtaccgg cctagagccg gtcggcatcc ttccggcaca cctgcacgat
     3781 ccgggcaacc ctgggtcgca gtcggtcacg ccatcgaccg gcttacgagg tccaggtcgc
     3841 agttggtccc gctatccgtc gccgattccg ggacgaatcg cgacctgagc gcgagaggcg
     3901 ccgggattcc aagacgaatc gcgatctggg cgcgagaggc cgcgctcagt cccagtggcc
     3961 gggctcggcc agcacgtgcg agcgcacgct cgcggcatcc gccgacacct ccgccgtgca
     4021 gtacagcagc gactcggtgg ggtagccgtg cgcgttgttg tcgcggatga tcgcccggtc
     4081 gtcgtcgggg tccagctcgg cgctgcgtgc gagcagctcc acccagcgac cggtccaccc
     4141 ctcgtcgtcc ggcctggcct cggcgaagtg cggcagccag gtctcgatgc gggcggtgcg
     4201 cgggtcgtcc aggtcgtcgt gcgcgatcat gtgcacgccg tgcctcaccg gtgtctcgcg
     4261 cagggtgtcg ccgtcccagt gcagcacccg cgagccggtc ggcgtcacct cgagcaggtt
     4321 gaacccgtgc atccgcaggg gtgcctcggg cgaacgcccg gcgaccgact cgagcgcgag
     4381 cgaaccgcgc gagagcacat cggcctcagg caggtccagc acgtcggcgc ggttcagcag
     4441 cacggccagc cggtgagtct gcgggtcgag cgccagccag gccccgcccg ctcggcggtc
     4501 gtggatgccg atgacacccg ggtacgcgtc cggccaccac tcacccaggt cgtcccacgc
     4561 acgctgcggg tcctcgtcgc gcacggccag cagccgggcc ccggcatccg ccacatcgat
     4621 caccacggtg cacacgtgca cgagtctagg gcggcccgga gcgcccggct ccgcggcatc
     4681 cgcccccgcc cgccgagcgc aactccgcag ttctgcacaa ctccgcagga ttccggcgga
     4741 tgcctgcgag gcagcgcaga tctgcggtga tgtgccacgc gccccacccg gccgtcacgc
     4801 gctggggcag aatcgagggg tgaacatcgt cgtgggagtc accggcggca tcgccgcgta
     4861 caaggcggtg cacctggtgc gcctgctgat caaggacggg cacgacgtga ccgtcatccc
     4921 caccgaggac gcgctgcgat tcgtcggact gcccacctgg gaggcgatca gccgcaaccc
     4981 ggtgaccacg agcgtgcacg aggacgtcgc gaaggtgcgg cacgtcgcgc tcggccaggc
     5041 ggcagagctg gtgatcgtcg cgccggcgac ggccaacacg atcgccaaga tgaccgcggg
     5101 cctggccgac gacctgctcg gcaccaccct gctcgccacc accgcgcccg tcgcgatcgc
     5161 ccccgccatg cacaccgaga tgtggcggca tcccgccacc caggcgaaca tcgacaccct
     5221 gcgcgagcgc ggcgtcgcga tcctcggccc cgccgagggg gagctcaccg gcggcgactc
     5281 ggggccg
//
LOCUS       DATMSX010000182        11743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_40683,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000182 DATMSX010000000
VERSION     DATMSX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40683"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VN041_05565"
     CDS             complement(<1..294)
                     /locus_tag="VN041_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28546.1"
                     /translation="MTPPTPHRATMKDVAALAGVSPKTVSNVVTGAFTVSEEKRDKVE
                     RAMRQLDFVPNLSARGMRTGRSGTIAVALPDLATAYSAELVHSLVAAAHQRGLA"
     gene            401..1423
                     /locus_tag="VN041_05570"
     CDS             401..1423
                     /locus_tag="VN041_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28547.1"
                     /translation="MRSGSAASADASRPSIYDVAERAGVSHMTVSRVLNHHPNIREST
                     RRRVTDAIAELNYAPSSIARALVRRRSLRIGAIVDNPGQYGPSSTLRAVEAAARENGY
                     AVSALSLEGRTAGIREGILDLVTEGVDALCVIAPRESSLQAVRQHAAALPTLVVKEEA
                     DALLHTVSVDQRAGARLAVGHLIELGHRRILHLAGPPDWFDARARTAAWRDALEDAGL
                     DAPDPVVGDWSSESGYLVGRALRLDGATAVFAANDQMALGLLHALAERGIRVPDDVSV
                     VGFDDVPDARHYTPPLTTVRQDFTALGRLVIRELLTAIDGGQPGHRELIRPELVVRAS
                     TSAVLR"
     gene            1581..3284
                     /locus_tag="VN041_05575"
     CDS             1581..3284
                     /locus_tag="VN041_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28548.1"
                     /translation="MTARKRITGILAVGVTAVLLLSGCSGNGPESADQASSSSAATPK
                     KGGTVYMLQNADFSYLDPARGWDGGVNAFYRLLYRGLTMQNAGDAKDPNAIVPDLAES
                     LGTPSDDGLTWTYKLKDDLKFDDGTPITSAEVKFGISRAWDPQIGIGSPYLKAVIDAP
                     GDYQGPYVSGDIPTIETPDAKTIVFHLKQPFPEFDNVLAQPNAVPFPKGSGGGDSFIK
                     DVIASGPYTLASYTPGSVIKLERNKYWDSKTDDVRKAYPDAWQVNLGIDPATIDERMI
                     SGQGDDADAIAGKISAATLPRLQTPQLKARVISAPAFCTTYLGMNTTRPPFDKVETRQ
                     AVNWALDRSAVQNASGGTQLADVATTIIPPAVSGHADFDLWKSKDGKGDPEKAKELLA
                     KAGLPDGFTFTMDIRSQPAMQAQAEAVQESLKRAGITVKLNVIDTSVYYETIGTPAQQ
                     HDAAITGWCPDWASSASTFIPPLFDGRKVTEKGNNNLSQLNDPAVNARIDEIMTMTDT
                     DAAKKAWGDLDKQILELSPIAPLVWEKGLFLPGTNIAGYRPSTNMTDLTIVGLKDPSK
                     G"
     gene            3287..4255
                     /locus_tag="VN041_05580"
     CDS             3287..4255
                     /locus_tag="VN041_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28549.1"
                     /translation="MTLTSSSLEIEAAPGADSESLSESAPTSGRRLVHAFVKDWAAVL
                     SSAYIVLIILMALAAPLLARITGWGPYEFDQGAIDPNMGAIPIGPAGGISAEHWFGVE
                     PGSGRDIFVRIAYGAQVSMAVALSATVITTVIGTVLGMLAGYFGGIIDTIISRVMEFM
                     MAFPALIFMIAILSALPSDNRIVLLVVVISLFGWPYLARIVRGQTLSIKEREFVEAAK
                     ASGAGSARIIFREILPNITSTILVMAALAVPGYVGTEAGLSFLGVGVVPPTPSWGQMI
                     ASAAPWYAVDPMYFVFPGAFLFLLVLSFVTLGDRVRAILVSGETRA"
     gene            4252..5250
                     /locus_tag="VN041_05585"
     CDS             4252..5250
                     /locus_tag="VN041_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28550.1"
                     /translation="MIRYITGRVLGMVVVLFLVTLFTYAIFFLLSPDPAAQICGKNCT
                     PELIDQIRTNLGLDQPFWTQFFTYVGGLFTGRTFVVAGAPVDCAAPCLGYSFQTSQLV
                     GDMIVQRLPISVTIALGAAVLWVLAGLVTGVLSAAKEGAFVDRFFAGLTLTAMSIPNY
                     VLALVLQFVLVVALGWLPFPQAVSFTDDPGRWFLNFLMPWVVLAAGYAATYTRLTRAN
                     VIETLQENFVRTARAKGLAPSLVWRRHALRPALTPIVTIFGLDLAGLLGGALITEKVF
                     GLNGVGKLTADSIAANDQPVIMAVTLLSAVFVIVGILVVDLLYSVIDPRVRTGGAQ"
     gene            5247..6131
                     /locus_tag="VN041_05590"
     CDS             5247..6131
                     /locus_tag="VN041_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28551.1"
                     /translation="MTGNARTPLLSVRDLTIGFPTANGTTSVVRGLGFDVPRGGAVGI
                     VGESGSGKSMTSLAVMGLLPKGGTATGSIVFDGRELIGLPDRVMRDVRGDRIAMIFQD
                     PLSSLNPYYKVGVQIAEAYRAHRPGVSRREARKVAIEAMDRVRIRDAAERVDHYPHQF
                     SGGMRQRIMIAMALSMEPDLIIADEPTTALDVTVQAQILALLDEIREETGAGLMLITH
                     DLAVVSEVTDHIVVMRDGEMVEQGAAEQIFCDPQADYTQRLLDAVPRIDDEIGELRTT
                     DVIVVPGSTRTTTTEERP"
     gene            6128..7009
                     /locus_tag="VN041_05595"
     CDS             6128..7009
                     /locus_tag="VN041_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28552.1"
                     /translation="MSTESAPLFRGTGLVKEYSRSRGIFKQRTVFRAVDDVTLEVHRG
                     ETLALVGESGSGKSTTARLAARLVDVTSGTVEFEGQDVTHLKGSDLRDFRSAVQVIFQ
                     DPYSSLNPKHSVERLVTAPLVYQKRKIPGGARAFARDLMARVGLNPDHSERYPSQFSG
                     GQAQRIGIARALAAGPRLVICDEAVSALDVSVQAQVINLLRELQADEGFGYLFIAHDL
                     AVVRQIATRVAVMHQGRIVETGPRDVVFDHPQDDYTKTLLQAVPRIDPEWDRRRREAQ
                     QARGAGAIATATEEEGR"
     gene            7006..8289
                     /locus_tag="VN041_05600"
     CDS             7006..8289
                     /locus_tag="VN041_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU28553.1"
                     /translation="MTVSYHLPGITVREQRLDAPLDWADPGAGTVSLLVRELVDPERA
                     GDDLPLMTYLQGGPGGANPRPLLRDGWIDEALRDYRVVLVDQRGTGGSSPLESADFSD
                     LDGAAGARLLSLHRADSIVRDLEHVRTSLYGGRRWATLAQSYGGFLTLTYLSQAPEAL
                     AACYVAGGIPGVPPRAADVYARTFDRVAAKTAELYRRFPEDAEAVARIADRLAEGDVQ
                     LPDGDALTVHRFQSLGIDLGMKPGHERMHWLVEKAFARPGRLSEGFLAEVLARSSSAG
                     NPLFWTLQESIYGDQESGPTGWAAQRERDRRPEFDPGRRPLLFTGEMAFPWMFDEVRL
                     LRGFRDAVHVLAEQVEWSPLYDRERLAANEVPLAAAVYFDDMYVDAGLQLESLSGIGN
                     AQYWVTNEFEHDGIGSGRVLSALRERVRDRGGEHR"
     gene            8286..9734
                     /locus_tag="VN041_05605"
     CDS             8286..9734
                     /locus_tag="VN041_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HWU28554.1"
                     /translation="MTVAPRTSVRPPYAGTRYPRLAEVPAFTDMIGQGWQEQTVLPDV
                     EPGTAEAAASHRARLSAAHPGRTLLFAAGTAPTRNDDAQYAFRADSGFVWLTGCQAEG
                     AVLVMSARPGGHDAVLYIPEPFRPGDSGFFSDAAHGELWVGPSAGLAEWAVALQITVE
                     PLSALEGRRSELRGAHAARSATDLAHEVDAELSAELERSVSILRMVKDDWEIGQLRSA
                     VDATVGGFDAVAREVPRAIAEGLGERWLQGTFDRHARTFGNGPGYSTIVGSGPHAPIL
                     HWVRCDGDVLPDAALLLDMGVEARSLYTADVTRTIPASGAFSPAQRAVHDLVERAHRA
                     GLEQIRPGVEYLDFHFAAMEVIAAGLHDWGLLPVSVDEALSPHGQQHRRYLACGIGHH
                     LGLDVHDCSQAHYEDYMGAPLQAGVVMTVEPGLYFHAHDLTVPPELRGVGVRLEDDVL
                     VTPHGSEVLSEALPIDADGVERWVRERLGTAA"
     gene            9731..>11743
                     /locus_tag="VN041_05610"
     CDS             9731..>11743
                     /locus_tag="VN041_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWU28555.1"
                     /translation="MSAVSAPRLRPFPLGDVRIGDDGMFARSRRQMLHLARAYPVDRL
                     LAVFRANAGLDVRGAEPPGAWEGFGHPDEQPWSEHDYPGREAAHTANLLRGHYAGHFL
                     SMLSLAAAGEADAGLRGKVDEFVAGLAEVQAALAASGRYSHPGFLAAYGEWQFSRLEQ
                     YAPYGEIWAPYYTCHKIMAGLLDAYELAGSTQALEIVTGMARWVGSRLSRLDAAQLQR
                     MWSLYIAGEFGGMNETLARLAAVTGEPEFLDTARLFDQRELIDAGRSGLDILDGMHAN
                     QHLPQLIGYVDEYEATGDREYLDAAQGVWGQVVPGRMYPHGGTGESELWGPAGSVARD
                     IGRRNAETCATYNLVKLSRRLFAHTLDPRYTDYIDRALSNHILGSRRDVDSDVSPEVT
                     YMFPVHPGALPEYDNIGTCCGGTGLENHVTQQASMFLRGEDGDLWLTQYTPAELDWTE
                     RGVRVVLEQEHPFASTVRVRVEPTDDPLALTVHLRIPDWVEGEPRATVAGRPLDLDVR
                     NGYASVRRTWRRGEALTLELPTALRAVPAVDDPHLVHLRYGPHVLVARSDSTTALELA
                     IAAARRPDGSLVSDADPAQALRDGGVTVDGIRFVPSWTGADARYHMYVRVADAVVGFA
                     GVQTEVPARQRADGSTLLDDVWASPGPVTVTEFAERVVTAVRDGLRD"
BASE COUNT         1780 a         4072 c         4067 g         1824 t
ORIGIN      
        1 ggcgagcccg cgctggtggg ccgcggcgac caggctgtgc accagctcgg ccgagtacgc
       61 ggtggccagg tccggaaggg cgaccgcgat cgtgcccgac cggccggtgc gcatgccacg
      121 ggcgctgagg ttcggcacga agtcgagctg ccgcatcgcc cgctcgacct tgtcgcgctt
      181 ctcctcgctg accgtgaacg cgcccgtgac gacgttcgag accgtcttgg gcgagacccc
      241 ggcgagggcg gcgacatcct tcatcgtcgc gcggtgcgga gtcggcggcg tcatgcgggc
      301 cagcctagtg cggggcgggg ccgtgtgttc ggaggaggcc gccgcgttcc aggcgggtag
      361 cctggcatgg tctgcgatca tgacggcgag gaggctacac gtgaggagcg gttcagcggc
      421 atccgctgat gcgtcgcggc cgagcatcta cgacgtcgcc gagcgcgccg gtgtctcgca
      481 catgaccgtg tcccgggtgc tgaaccacca tcccaacatc cgcgagtcca cgcgccgacg
      541 tgtcacggat gccatcgccg agctcaatta cgcgcccagc tcgatcgccc gggcgcttgt
      601 gcgccgccgc tccctgcgca tcggcgcgat cgtggacaat cccgggcagt acggaccgag
      661 cagcacgctg cgcgcggtcg aggccgctgc ccgagagaac gggtacgcag tgagcgcgct
      721 gtcgctggag gggcggacgg cgggcatccg ggagggaatc ctcgatctgg tcaccgaagg
      781 cgtcgacgcc ctgtgcgtga tcgcgccgcg cgagtcctcg ctgcaggctg tgcgtcagca
      841 cgccgccgcg ctgccgactc tggtcgtgaa ggaggaggcc gatgcgctgc tgcacaccgt
      901 gtcggtggac cagcgcgcgg gagcccgact ggccgtcggg catctgatcg aactcggtca
      961 ccgccggata ctccatctgg ccgggcctcc ggactggttc gacgcccgcg cccgcaccgc
     1021 ggcctggcgg gacgcgctgg aggatgccgg tctggacgct ccggacccgg tggtggggga
     1081 ctggtcatcc gagtccgggt acctggtggg gcgcgcgctc cggctggacg gcgcgacggc
     1141 ggtgttcgcg gcgaacgacc agatggcgct cggtctgctg cacgcccttg cggagcgggg
     1201 catccgtgtg cccgacgatg tcagcgtggt ggggttcgac gatgtgcccg acgcccgcca
     1261 ttacacgcca ccactgacca ctgtgcgcca ggacttcacc gcgttgggcc gtctggtcat
     1321 ccgagaactg ctcaccgcga tcgacggagg ccagcccggt caccgggagc tgatccgccc
     1381 cgagctggtc gtccgagcgt cgacctcagc cgtcctccgg taaccgcggg gctcagcatc
     1441 cggggcggtc cgtgacgacc cgatcgttgc gctcgcgttc tgttctcgct aacatcgtcg
     1501 ctcagtaagg ttttacattg cagtgtcatg cgctgtgcct tagggcatct atgcaacgga
     1561 gcagagaagg gatcggtccg atgaccgcca ggaagagaat cacaggcatc ctcgccgtcg
     1621 gtgtgacagc agtgctgctg ctgtccggat gcagcgggaa cggccccgaa tcggcagacc
     1681 aggccagcag ttcgtccgct gccaccccga agaagggtgg cacggtgtac atgctgcaga
     1741 acgcggactt ctcctacctc gacccggccc gcggctggga cggtggcgtg aacgcgttct
     1801 accggctgct gtaccgcggc ctgaccatgc agaacgccgg tgacgccaag gacccgaacg
     1861 ccatcgtccc cgacctggcc gagtccctcg gcacaccgtc ggatgacggc ctgacgtgga
     1921 cctacaagct caaagacgac ctcaagttcg acgacggcac cccgatcacc tccgccgagg
     1981 tcaagttcgg gatctcccgc gcctgggacc cccagatcgg catcggatcg ccctacctga
     2041 aggcggtcat cgacgcgccc ggcgactacc agggtccgta cgtctccggt gacatcccga
     2101 ccatcgagac cccggacgcc aagaccatcg tcttccacct gaagcagccg ttccccgagt
     2161 tcgacaacgt tctcgcccag ccgaacgcgg tgcccttccc gaagggctcc ggtggaggag
     2221 actccttcat caaggacgtg atcgcctccg gtccgtacac cctcgcctcg tacacaccgg
     2281 gcagcgtcat caagctcgag cgcaacaagt actgggactc gaagaccgac gacgtgcgca
     2341 aggcctatcc ggatgcctgg caggtcaacc tcggcatcga ccccgcgacc atcgacgagc
     2401 gcatgatctc cggtcagggc gatgacgccg acgcgatcgc cggcaagatc agcgccgcga
     2461 ccctgccgcg cctgcagacc ccgcagctga aggcgcgcgt gatcagcgcg ccggcgttct
     2521 gcaccaccta cctgggcatg aacaccacca ggccgccgtt cgacaaggtc gagacgcgtc
     2581 aggccgtgaa ctgggcgctc gaccgttcgg ccgtccagaa cgccagcggt gggacgcagc
     2641 tggcggacgt cgccaccacg atcatcccgc cggccgtcag cggtcacgcc gacttcgacc
     2701 tctggaagag caaggacggg aagggcgacc cggagaaggc gaaggagctg ctcgccaagg
     2761 ccggcctgcc ggacggcttc accttcacca tggacatccg ctcgcagccg gccatgcagg
     2821 cgcaggccga ggctgtgcag gagtcgctca agcgcgccgg catcaccgtc aagctgaacg
     2881 tcatcgacac ctcggtgtac tacgagacca tcggcacccc ggctcagcag cacgatgccg
     2941 ccatcaccgg atggtgcccg gactgggcct ccagcgccag caccttcatc ccgccgctgt
     3001 tcgacggccg caaggtcacc gagaagggca acaacaacct ctcgcagctc aacgacccgg
     3061 cggtcaacgc gcgcatcgac gagatcatga cgatgaccga caccgatgcg gccaagaagg
     3121 cgtggggcga tctggacaag cagatcctgg agctgtcgcc catcgcgccg ctggtgtggg
     3181 agaagggtct gttcctgccc ggcaccaaca tcgcggggta ccgccccagc accaacatga
     3241 ccgacctcac catcgtcggt ctgaaggacc cctcgaaggg ctgaccatga ccctcacctc
     3301 gagttcactc gagatcgagg ccgcaccggg tgccgattcc gagtccctct cggaatcggc
     3361 acccacctcc ggccgccggc tcgtgcacgc attcgtcaag gactgggcgg cggtgctgtc
     3421 ctccgcctac atcgtcctga tcatcctgat ggcgctggcc gccccgctcc tcgcccggat
     3481 cacggggtgg gggccgtacg agttcgatca gggagccatc gacccgaaca tgggcgcgat
     3541 cccgatcgga cccgccggcg gcatcagtgc tgagcactgg ttcggcgtcg agcccggcag
     3601 cggccgggac atcttcgtcc gcatcgcgta cggagcgcag gtgtcgatgg ccgtcgcgct
     3661 gtcggccacc gtcatcacca ccgtgatcgg caccgtgctc ggcatgctgg ccggctactt
     3721 cggcggcatc atcgacacga tcatctcccg cgtcatggag ttcatgatgg ccttccccgc
     3781 gttgatcttc atgatcgcca tcctctcggc gctcccctcc gacaaccgga tcgtgctgct
     3841 cgtcgtggtg atctcgctgt tcgggtggcc ctacctcgca cgcatcgtgc gcggccagac
     3901 gctctccatc aaggagcggg agttcgtcga ggcggcgaag gcgtccggcg caggctccgc
     3961 gcgaatcatc ttccgggaga tcctgcccaa catcacctcg acgatcctcg tcatggcggc
     4021 tctcgcggta cccggctacg tcggcaccga ggcgggcctc tccttcctcg gcgtcggggt
     4081 cgtgcccccc acgccgtcct gggggcagat gatcgcctcc gcggcgccgt ggtacgcggt
     4141 cgacccgatg tacttcgtct tccccggtgc gttcctgttc ctgctggtgc tgtccttcgt
     4201 cacgctcggg gaccgcgtgc gggcgatcct ggtcagcggg gagacgcgcg catgatccgc
     4261 tacatcaccg gccgcgtgct cggcatggtc gtcgtgctgt tcctggtgac gctgttcacc
     4321 tacgcgatct tcttcctgct ctcgccggac ccggccgcac agatctgcgg caagaactgc
     4381 acaccggagc tgatcgatca gatccgcacg aacctcgggt tggaccagcc gttctggacg
     4441 cagttcttca cctacgtcgg cgggcttttc accgggcgca ccttcgtggt cgccggtgcg
     4501 cccgtggact gcgcggctcc gtgcctgggc tattccttcc agaccagcca gctggtcggt
     4561 gacatgatcg tgcagcgact gccgatctcg gtgaccatcg ccctgggggc agcagtgctg
     4621 tgggtgctgg cgggactcgt caccggcgtg ctcagcgccg cgaaggaggg cgccttcgtc
     4681 gaccgcttct tcgcagggct caccctcacg gcgatgtcga tcccgaacta cgtgctcgct
     4741 ctcgtgctgc agttcgtgct cgtcgtcgcc ctgggctggc tgcccttccc gcaggcggtc
     4801 tcgttcacgg acgaccccgg ccgatggttc ctgaacttcc tgatgccatg ggtcgtgctg
     4861 gcggcgggat acgcagccac ctacacacgg ctcacccgcg ccaacgtcat cgagacgctg
     4921 caggagaact tcgtgcgcac ggccagggcg aagggcctcg ctccctcgct ggtgtggcgg
     4981 cgtcacgcac tgcgcccggc tctgaccccg atcgtgacca tcttcggact cgacctggcg
     5041 ggactgctgg gcggtgcgct gatcaccgag aaggtgttcg gcctcaacgg cgtgggcaag
     5101 ctcaccgccg actcgatcgc cgcgaacgac cagccggtga tcatggcggt cacgctgctg
     5161 tcggccgtgt tcgtgatcgt gggcatcctg gtcgtcgacc tgctctactc ggtgatcgat
     5221 cctcgggtgc ggacaggagg tgcacaatga ccgggaacgc caggaccccg ctgctgtcgg
     5281 tgcgcgatct gacaatcggc tttccgaccg ccaacggcac cacctcggtc gtgcgcggcc
     5341 tcggcttcga cgtcccgcgc ggcggtgcgg tcggcatcgt cggcgaatcc ggatcaggca
     5401 agtcgatgac cagcctggcc gtcatggggc tgctgccgaa gggcggaacc gcgaccgggt
     5461 cgatcgtctt cgacggccgc gagctcatcg gcctgccgga ccgggtgatg cgcgatgtgc
     5521 gcggcgaccg catcgcgatg atcttccagg acccgctctc ctcgctgaac ccgtactaca
     5581 aggtgggggt tcagatcgcc gaggcctatc gggcgcaccg cccgggtgtc agccgtcggg
     5641 aggcgcgcaa ggtcgccatc gaggccatgg acagggtgcg gatccgtgac gccgcggagc
     5701 gcgtcgacca ctacccgcac cagttctccg gcggcatgag gcagcgcatc atgatcgcga
     5761 tggccctgag catggagccc gacctgatca tcgccgacga accgaccacc gcgctggacg
     5821 tcaccgtgca ggcgcagatc ctcgctctgc tcgatgagat ccgcgaggag accggagccg
     5881 gactgatgct gatcacgcac gacctggctg tggtcagcga ggtcaccgat cacatcgtcg
     5941 tgatgcgcga cggcgagatg gtggagcagg gtgctgcgga gcagatcttc tgcgatccgc
     6001 aggcggacta cacgcagcgt ctgctggatg ccgtgccgcg catcgatgac gagatcggtg
     6061 agctgcggac caccgacgtg atcgtggtcc ccggatcgac ccgcacgacc acgacggagg
     6121 agcgaccatg agcaccgaat cggctccgct gttccggggc accgggctgg tgaaggagta
     6181 ctcgcgttcg cgggggatct tcaagcagcg cacggtgttc cgcgcagtcg acgacgtcac
     6241 cctggaggtg caccgcggcg agacgctcgc cctggtcggc gagtccggat cgggcaaatc
     6301 cacgaccgcg cgtctggcgg cccggctcgt cgacgtcacc agtggcaccg tcgagttcga
     6361 gggccaggac gtcacgcatc tgaagggctc cgacctgcgc gacttccgca gcgccgtgca
     6421 ggtgatcttc caggacccgt actcgtcgct gaacccgaag cacagcgtgg agcgtctggt
     6481 gaccgcaccg ctggtgtacc agaagcggaa gatcccgggc ggggcccgtg cgttcgcgcg
     6541 tgatctgatg gcccgtgtcg gcctgaaccc cgaccacagc gagcgctacc cctcccagtt
     6601 ctccggcggt caggcgcagc gcatcggcat cgcgagggcg ctggcggccg gcccccggct
     6661 ggtgatctgc gacgaagcgg tgtccgctct ggacgtgtcg gtgcaagccc aggtgatcaa
     6721 cctgctgcgg gagctgcagg cggatgaggg cttcggctac ctgttcatcg cacacgatct
     6781 cgccgtggtg cgccagatcg cgacccgggt cgcggtgatg caccagggcc ggatcgtgga
     6841 gaccgggccg cgcgacgtcg tgttcgacca cccgcaggac gactacacca agaccctgct
     6901 gcaggccgtg ccgcgcatcg acccggagtg ggatcgacgc cggcgtgagg ctcagcaggc
     6961 gcgaggcgcc ggggccatcg cgaccgcgac cgaggaggag ggccgatgac ggtgtcgtac
     7021 cacctgccgg ggatcacggt acgcgagcag cgactggacg cgcccctgga ctgggcggat
     7081 ccgggcgccg ggaccgtcag cctgctggtc agggagctcg tggatccgga gcgggccggc
     7141 gatgacctgc cgctgatgac gtacctgcag ggcggcccgg gcggcgccaa tccgcggccc
     7201 ctgctgcggg acggatggat cgacgaggcg ctgcgcgact accgggtcgt gctcgtcgac
     7261 cagcgcggca ccggaggcag cagtccgctg gagtcagctg acttctcaga cctcgacggg
     7321 gcggccgggg cccgcctgct ctcactgcac cgtgccgact cgatcgtgcg cgacctcgag
     7381 catgtgcgta cgtcgctgta cggtggccgg cgctgggcga cccttgcgca gagctacggc
     7441 ggcttcctca cgctgaccta tctgtcgcag gcgccggagg cactagccgc ctgctatgtc
     7501 gcgggtggaa tccccggcgt cccgccccgc gcggccgacg tgtacgcccg caccttcgat
     7561 cgggtggcgg cgaagaccgc cgagctgtac cgcagattcc cggaagacgc cgaggcggtc
     7621 gcgcggatag ccgaccggct ggccgaaggc gacgtgcagc tgcccgacgg cgatgcgctc
     7681 acggtgcatc gtttccagtc actgggaatc gatctcggca tgaagcccgg gcacgagcgc
     7741 atgcactggt tggtcgagaa ggcgttcgct cgccccggtc gactctccga gggcttcctc
     7801 gcggaggtgc tcgcccgaag ctcgagcgcg ggcaacccgc tgttctggac cctgcaggag
     7861 tccatctacg gtgaccagga gagcgggccg acaggatggg ccgcgcagcg ggagcgcgat
     7921 cggcgtcccg agttcgaccc cggccgtcgg ccgctgctgt tcaccgggga gatggccttc
     7981 ccgtggatgt tcgacgaggt gcggcttctg cgaggcttcc gcgacgccgt gcacgtgctc
     8041 gcggaacagg tcgagtggag cccactgtac gaccgggagc gtctcgcggc gaacgaggtg
     8101 ccgctggcag ccgccgtcta cttcgatgac atgtacgtcg atgcggggct gcagctggag
     8161 agcctgtccg gcatcggcaa cgcgcagtac tgggtgacca acgagttcga gcacgacggc
     8221 atcggctccg gccgggtgct ctcggcgctg cgcgaacgag tacgcgatcg tggaggagaa
     8281 cacagatgac agtcgcacct cggacctcgg tccgcccgcc ctacgccgga acccgctacc
     8341 cgcggctggc agaggtgccc gccttcacag acatgatcgg ccagggctgg caggagcaga
     8401 ccgtcctgcc cgatgtggag ccggggactg ccgaggcggc ggcctcccac cgtgctcgtc
     8461 tcagcgccgc tcatccggga cgcaccctgc tcttcgccgc aggcaccgct cccactcgca
     8521 acgacgacgc gcagtatgcg ttccgtgccg acagcggctt cgtctggctc accggatgcc
     8581 aggccgaggg ggccgtcctg gtgatgtccg cgcggccggg gggacacgac gccgtgctct
     8641 acatccccga gccgttccgc ccgggtgact cgggcttctt ctcggatgcc gcccacggcg
     8701 agctctgggt cgggccatcc gcgggccttg cggagtgggc ggtggcgctg cagatcacgg
     8761 tggagccgct gagcgcgctg gagggtcggc gcagcgagtt gcgcggcgcg cacgccgccc
     8821 ggtcggcgac cgacctggct cacgaggtgg acgccgaact gtccgccgag ctggaacggt
     8881 cggtgtcgat cctgcgaatg gtgaaggacg actgggagat cgggcagctc cgctcggccg
     8941 tggacgccac cgtcgggggc ttcgacgccg tcgcccggga ggttccgcga gccatcgcag
     9001 agggtctcgg ggagcgctgg ctgcagggca ccttcgaccg gcatgcccgc acgttcggca
     9061 atgggccggg atactccacc atcgtcgggt cgggcccgca cgcgcccatc ctgcactggg
     9121 ttcgctgcga tggcgacgtg ctgccggacg cggccctgct gctggacatg ggagtcgaag
     9181 cacgctcgct gtacacggcc gacgtcacgc gcaccatccc ggcctccggg gccttctcgc
     9241 ccgcccagcg agccgtgcac gacctggtcg agcgcgccca ccgcgccggg ctggagcaga
     9301 tccgtcccgg cgtcgagtac ctggacttcc acttcgctgc gatggaggtg atcgccgccg
     9361 gcctgcacga ctgggggctg ctgccggtct ccgtggacga ggcgctctcc ccgcacgggc
     9421 agcagcaccg ccgctacctc gcgtgcggca tcgggcacca cctcgggctg gatgtgcacg
     9481 actgcagcca ggcccactac gaggactaca tgggcgcacc gctgcaggcc ggagtggtga
     9541 tgacggtgga gcccggactg tacttccacg cccacgacct caccgtgccg cccgagctgc
     9601 gcggtgtcgg ggtgcgtctg gaggacgacg tgctggtcac gccgcacggc tcggaggtgc
     9661 tctccgaggc gctgccgatc gacgcggacg gcgtcgagcg gtgggtgcgg gagcgactcg
     9721 ggaccgcggc atgagcgcag tctccgcacc gcgactgcgc ccgttcccgc tcggcgatgt
     9781 gcggatcggg gacgacggga tgttcgcccg ctcccgtcgg cagatgctgc atctggcccg
     9841 cgcgtacccc gtcgaccggc tgctggcggt gttccgcgcc aacgccgggc tggacgtgcg
     9901 cggcgcggag ccgcccgggg cctgggaggg cttcggtcac ccggacgagc agccatggag
     9961 cgaacatgac tatccgggcc gggaggcggc gcacaccgcc aacctgctgc gggggcacta
    10021 cgccggtcac ttcctctcca tgctctcgct ggcggcggcg ggtgaggcgg atgccggact
    10081 gcgcggcaag gtcgacgagt tcgtcgccgg tctcgccgag gtccaggcgg cgctggccgc
    10141 cagcggacgg tactcgcatc cgggcttcct cgccgcctac ggcgaatggc agttctcgcg
    10201 actggagcag tacgcgccgt atggcgagat ctgggcaccg tactacacct gccacaagat
    10261 catggcgggg ctgctcgacg catacgaact ggcgggcagc acgcaggcgc tggagatcgt
    10321 gaccggcatg gcacgctggg tcgggtcccg gctgagccga ctggatgccg cacagctgca
    10381 gcggatgtgg tcgctgtaca tcgccggtga gttcggcggg atgaacgaga cgctggcgcg
    10441 gctggccgcc gtcaccgggg agcccgagtt cctcgacacg gcgcggctgt tcgaccagag
    10501 ggagttgatc gacgccggcc gctccggcct cgacatcctc gacggcatgc acgccaatca
    10561 gcatctgccg cagctgatcg gatacgtgga cgagtatgag gccacgggtg atcgtgagta
    10621 cctggacgca gcacagggcg tctggggtca ggtcgtgccg ggccggatgt acccgcacgg
    10681 cggcaccggc gagagcgagc tctggggccc cgccgggtcg gtcgcgcgtg acatcgggcg
    10741 gcgcaacgcc gagacctgcg cgacgtacaa cctcgtcaag ctgtctcgca ggctgttcgc
    10801 gcacaccctc gaccccaggt acacggacta catcgaccgg gcactgagca accacatcct
    10861 cggttcgcgg cgcgacgtcg actcggacgt cagccccgag gtgacctaca tgttccccgt
    10921 gcaccctggc gcgctgcccg agtacgacaa catcggcacg tgctgcggcg ggaccggcct
    10981 ggagaaccat gtgacgcagc aggcgagcat gttcctccgc ggggaggacg gcgacctgtg
    11041 gctcacccag tacacgccgg ccgagctgga ctggaccgag cgcggggtgc gcgtcgttct
    11101 ggagcaggag catccgttcg cctccaccgt gcgcgttcgc gtcgagccca ccgatgaccc
    11161 gctggcgctg accgtgcacc tgcgcatccc cgactgggtg gagggcgaac cgcgcgcgac
    11221 agtggccggc cggccactcg acttggacgt gcggaacggg tacgcctcag tgcgccggac
    11281 ctggcgccga ggagaggcgc tgacgctgga gctccccacc gctctgcgtg ccgtccccgc
    11341 tgtcgacgac ccgcacctgg tgcacctgcg ctacgggccg cacgtgctgg tggcgcgctc
    11401 cgacagcacc accgccctcg agctcgcgat cgcagcggcc cgccgtccgg acggctcgct
    11461 cgtcagcgac gctgaccccg cacaggcgct ccgcgacggc ggagtgaccg tggacggcat
    11521 ccgcttcgtg ccgtcgtgga cgggggccga tgcgcggtac cacatgtacg tgcgggtggc
    11581 ggatgccgtg gtcggcttcg ccggcgtgca gacggaggtt ccggcgcggc agcgggcgga
    11641 cggcagcaca ctgctcgacg atgtctgggc cagccccggg ccggtcactg tgaccgagtt
    11701 cgccgagcgc gtcgtcaccg cggtgcgcga cgggctgcgg gac
//
LOCUS       DATMSX010000183        10140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_30986,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000183 DATMSX010000000
VERSION     DATMSX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30986"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VN041_05615"
     CDS             complement(<1..674)
                     /locus_tag="VN041_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWU28556.1"
                     /translation="MIPMLDWGDIFSFQDYGDLLVLLTNSLIAGAVLGIVGGLVGVFV
                     MQRDLAFAVHGVSELSFAGAAAALLFGGSVVLGSIGGALVAAMLIGILGSRARDRNSI
                     VGVLMPFGLGLGILFLSLVQGRTANRFGLLTGQIVAVSSPDLGWLIGISLAVLVGLLL
                     MWHPLRFDSLDPESAAARGVPVRAVSLLFMVLLGLIVAVSVHIIGALLVLALLVTPAA
                     AAMRVSA"
     gene            complement(671..1537)
                     /locus_tag="VN041_05620"
     CDS             complement(671..1537)
                     /locus_tag="VN041_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWU28557.1"
                     /translation="MSRASAAAGASGAAAPGAPVLGISAAALHRGGRELWAGLDLEVQ
                     PGEFIAVLGPSGSGKTTLLRSILGLQSLSAGSIRVGGTPVRRGDSRIGYVPQQRPLPP
                     DTSMRARDLIALGVTGTRFGLPIPRRDDRARVDALLEGVGAAHYADRRVGLLSGGEQQ
                     RLRVGQALADQPMLLLCDEPLSNLDLANQRGITEIIDRQRHEHGAAVLFVTHDVNPIL
                     GMVDRILYIAGGRFVLGTPDEVLQTSVLSALYGTPVFVMRTGGRLVVVGVPDAEPHHD
                     HDHEEPVSGGAA"
     gene            complement(1534..2562)
                     /locus_tag="VN041_05625"
     CDS             complement(1534..2562)
                     /locus_tag="VN041_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWU28558.1"
                     /translation="MRKILVSAALAASSLLVLAGCAAGPADGSKDGGSGNGRVQVVAS
                     TNVYGDIVRQIASDRADVTSLIDDAAKDPHSYEATARDRLAVQKADLIIENGGGYDSF
                     METLREGSDAPVVVAAEFSHDYPGDPHEHEDAATPEPTGDAHDDHADEHDDHGDADDA
                     HAGHDHIEGFNEHVWFDVHTITHVVEQVTTDLVKLDVAGKADYEAASKKFIGELNGFE
                     TQLEGLHTELEGTKVVMTEPLPGLLAAAAGLDDVTPDGFASAVEEGNDVPPAILLETT
                     RLISSGEVKAVLANAQTGGGETDKVEQAAKAAGVPVLTFSELLKSDQTYAEWMHAAIT
                     DLAAALHR"
     gene            2706..3608
                     /gene="mshB"
                     /locus_tag="VN041_05630"
     CDS             2706..3608
                     /gene="mshB"
                     /locus_tag="VN041_05630"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HWU28559.1"
                     /translation="MAPVAHVLLAHAHPDDESCATGGTIARLLAEGHGVTVVTSTQGE
                     QGRIAVPELQHLSVDEDDQLGPERARELSDAMTALGVTDHRYLGGAGRFRDSGRMGIP
                     ANDRSDSFWQADLDEAGLLMAEIIRETRPTAVITYDPNGGYGHPDHIQTHRVTMRGIE
                     LAADASAASGLPVWDVPLVYWTAVPRELIVADLTELAGHAEELGLWVDPDPAEYPDGV
                     HDDDEIDVEFDVSAHLAAKTAALACHRTQLTVKGDCWVLASGRGMRIQNREWFIRVKG
                     GRRRTPGYETELLPQQPTTEPEAD"
     gene            3608..4726
                     /locus_tag="VN041_05635"
     CDS             3608..4726
                     /locus_tag="VN041_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU28560.1"
                     /translation="MSRSTLALDDRSTQRARVVGALHEQRKSRSDLVEQFGVSSSLIT
                     RLVRGLIDDGILREVGAQAQAIGRPPVRLEVVRDHGALLAVSCTAEGLRARCVNLHGG
                     IIAETSTAPIAGVVSPESIVETVAQLIARTPRVLGIGVAVPGVVDAETGDVSAAPDLG
                     WTTEVPLRTMLRERFGVVVTIDNDVNLMMASERRTLDGRAARNAVYLYLGARGIGAGI
                     IAAGRLIEGDHGAAGEIGLSPLSANDRTDFEDTTSTLAIAARLEAAGHTGSEPAVDRL
                     LALADAGDPAAVRIRADVLTAFSHAIAVITALLDPHVILLGGHARAFREAEHREMLQP
                     LRGELPFLPDVRSAHPKPDAILDAALNRCWQQLLAGGL"
     gene            4798..5898
                     /locus_tag="VN041_05640"
     CDS             4798..5898
                     /locus_tag="VN041_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28561.1"
                     /translation="MKAHITRRRLAAFATFATAGALLLSGCAGDVAPPKSEAPTDGGS
                     KVTPSESLTIYTGRDKDEVAWVVSEFEKANPDYAGKVTTVIAGAQDNLDRMRAEAGNP
                     QAGFLWGGTQQQFEQAAKEGLLAAYEPKNGDAVDAKYKDPKNQWIAEMLLPEVIIYNS
                     DLLDEKSAPQDWADLAEPEWKDKIVIRDVMPSGTMRTIYASMVYSAFAKDSKPDAGYD
                     LLKKIDANTVSYAATPDDMYTQLDQGTGQVTVWNMQDALIQPLKNNRPWGYVMPKSGA
                     PVLVDGVGIIKNEKQQAAAQAFMDFLMEPKMQAKLATDYYQIPAMDIPKADQPDWMKD
                     FKLKEMKIDWAVFAEHQDEWMTYWADNIKDKG"
     gene            5907..6992
                     /locus_tag="VN041_05645"
     CDS             5907..6992
                     /locus_tag="VN041_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28562.1"
                     /translation="MVSVTLNQLRKSFARSGTVVDGIDLTIADGEFFTLLGPSGCGKS
                     TTLRMIAGFEEPDDGTIHFDDRDVTYAPANKRDVGLVFQNYALFPHMSVTRNVGFGLD
                     VRKVQKAEASRRISDVLAQVGLSAQAGARVDELSGGQQQRVALARALVFRPQLLLLDE
                     PMSNLDAKLRLEMRGALRDLHAETGITSVYVTHDQAEALAMSTRIAVMNTAVVHQVGT
                     PDEVYSRPRTAFVAGFLGRNNLLPATVRRVDGGRASLELADGSALEVLVENAAPGVSF
                     EPGSALIATVRAESLRPAASGETANVVSGTVADIDFVGYSLSCELDTPLGRIKVDLTG
                     THARPQRGDALRVVLPADAAYCVPAEQ"
     gene            6992..8734
                     /locus_tag="VN041_05650"
     CDS             6992..8734
                     /locus_tag="VN041_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWU28563.1"
                     /translation="MTTLTNAQRVTGPSKRPPWIRRRWSTVLTLILLMVVLLGYVIGP
                     MIATARRSVSQDKAAVLGFSFKAWSDFFANPTQTSAIGGSIVLSLLSVLCAGLLGTLV
                     AILLTRWEFPGRRICQVLALLPIALPPLMGVWSFKLLFGVGGRIPFAMQEWFGLDQKM
                     FWLQGLAGVLIVHAMTMYPYFFLTVSAALSNADASLEEAAESMGAGRARVWAKVVLPM
                     LTPALVAGSLITFMSAMSSYTAPLLFQYTDVMTQQIVIAKTNGDMRLASIISTTLAVI
                     SIVFLAIIRSYEQRKVYRTQSKGGGRKRSTVTSAPVKVLLFVIAVPTTVFLVLPIIMI
                     AVLSFTAKGGWLRSVLPTGYTLEWWAQLFDGAEFWRPVTNSLLMSVIAVGGAMILGAG
                     AAYVMSRMHFRGKLALDIAIMLPWALPGTVVAINLITAFSSPSPFAFGQVLVGTFAIL
                     PLAYFVRFNPLIFRSTFASFSQLDTNLEDAARSLGASWWYAFRRVVLPLISRGIMAGA
                     LLAFVDGVGEFVASILIYTPAWVPLSIAINNENYQGNIGTGSVYGMIQVLLVLGVLIV
                     TRRMEAKQALAPAL"
     gene            8792..>10140
                     /gene="argH"
                     /locus_tag="VN041_05655"
     CDS             8792..>10140
                     /gene="argH"
                     /locus_tag="VN041_05655"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWU28564.1"
                     /translation="MNRDEIIQRDGDAFPGESYTTAVLRPAYDQAKRMLLPSMIEIHR
                     AHLVMLHEAGILNDADAAAIARSIDELDLDALAASEYNGQFEDLFFTVEHEMMRIGGE
                     VTGSLHTARSRNDMGMTLYRMVMRRQLLRVLDAAGELYETLVRLGAEHADTLSLEHTH
                     TQPAQPSILGHRFLAIADVLGRDIRRVQQAYESLDYSPMGGAALTGTGFGIDRDHMAR
                     LLGFRGLVENSYDAVAATDYIAHSAIALQLMAIDTGRSCVDFLTWCTAEFGLYRVAAP
                     YVQISSIMPQKRNPVSIEHSRSLLSAASASAATVLTMMHNTPFGDIVDTEDDLQPYAW
                     RAVEMLTDVLQLLTGVLGTIQVNTAVMRERALSSFANATELADTLVRDGGLTFTQAHT
                     VVSRIVREADAAGITDIRSIDPARVHEVTTEVAGHDVTLTPEDLARALDADNFVAVRT
                     "
BASE COUNT         1614 a         3623 c         3375 g         1528 t
ORIGIN      
        1 gccgacaccc gcatggcagc ggccgcgggc gtgacgagca gcgcgagcac gagcagcgcc
       61 ccgatgatgt gcacgctcac ggccacgatc agcccgagca gcaccatgaa cagcaggctg
      121 acggcgcgca caggcacgcc gcgcgcggcg gcggactccg ggtcgaggga gtcgaagcgc
      181 agcggatgcc acatcagcag caggccgacg agcaccgcca ggctgatgcc gatcagccag
      241 cccaggtcgg ggctggagac cgcgacgatc tgcccggtga gcagtccgaa gcggttggcc
      301 gtgcgcccct gcaccaggga gaggaacagg atgcccaggc ccaggccgaa gggcatgagc
      361 acgcccacga tcgaattgcg gtcccgcgcg cgggaaccga ggatgccgat cagcatggcc
      421 gccacgagcg ccccaccgat cgaacccagc acgacgctcc cgccgaacag cagagccgcc
      481 gcggcgcccg cgaacgagag ctcgctcacg ccgtgcaccg cgaaggccag atcccgctgc
      541 atgacgaaga cgccgaccag cccgccgacg atgccgagca cggcgccggc gatcagcgag
      601 ttggtcagga gcacgagcag gtcgccgtag tcctggaacg agaagatgtc gccccagtcg
      661 agcatcggga tcatgccgca cctcccgaca cgggctcttc gtggtcatgg tcgtgatgcg
      721 gctcggcatc cggcacaccg accaccacga gccgaccgcc ggtgcgcatc acgaacaccg
      781 gcgtgccgta cagcgcgctg agcacgctgg tctgcagcac ctcgtcgggt gtgccgagca
      841 cgaaccggcc gcccgcgatg tagaggatgc ggtcgaccat gccgaggatg ggattgacgt
      901 cgtgcgtgac gaacagcacg gcggccccgt gctcgtggcg ctgcctgtcg atgatctcgg
      961 tgatgccgcg ctggttggcc aggtcgaggt tcgacagcgg ttcgtcgcag agcagcagca
     1021 tgggctgatc ggcgagcgcc tgcccgaccc tcagccgctg ctgctcgccg cccgagagca
     1081 ggcccactcg gcgatcggcg tagtgcgcgg cgcccactcc ctcgagcagg gcgtcgacac
     1141 gcgcccgatc gtcgcggcga gggatgggaa gcccgaaccg cgtccccgtc acgccgaggg
     1201 cgatcaggtc tcgcgcccgc atgctcgtgt ccgggggcag cggacgctgc tggggcacgt
     1261 agccgatgcg ggagtcgccc cgacgcaccg gcgttccacc gacgcggatg ctgccggcgg
     1321 acagcgactg gagacccagg atgctgcgca gcagagtggt cttgccggaa ccggacgggc
     1381 cgagaaccgc gatgaactcc cccggctgca cctccaggtc gagccccgcc cacagctctc
     1441 ggccaccgcg gtgcagcgcg gcggccgaga tgccgagtac gggtgctccc ggtgcggccg
     1501 cgcccgaggc gccggccgct gcggaagcgc ggctcatcgg tggagagcgg cggcgaggtc
     1561 cgtgatcgcg gcgtgcatcc actcagcgta cgtctgatcc gacttcagca gctcgctgaa
     1621 ggtcagcacc ggcaccccgg cggccttcgc cgcctgctcg accttgtcgg tctcgccgcc
     1681 accggtctgc gcgttcgcca gcacggcctt gacctctccg gaggagatca gacgcgtcgt
     1741 ctcgagcagg atggccggcg gcacgtcatt gccctcctcc acggcggatg cgaagccgtc
     1801 gggagtcaca tcgtcgagcc ctgcggccgc cgccagcagc ccgggcagcg gctcggtcat
     1861 cacgaccttc gtgccctcga gctcggtgtg cagcccctcc agctgggtct cgaagccgtt
     1921 gagctcgccg atgaacttct tggacgccgc ctcgtagtcg gccttgcctg ccacgtcgag
     1981 cttcacgagg tcggtggtga cctgctccac gacgtgggtg atggtgtgca cgtcgaacca
     2041 cacgtgctcg ttgaagccct cgatgtgatc gtgaccggcg tgggcgtcat ccgcgtcgcc
     2101 gtgatcgtcg tgctcgtcgg cgtggtcgtc gtgcgcatcg ccggtgggct cgggcgtcgc
     2161 tgcgtcctcg tgctcgtggg ggtcgcccgg gtagtcgtgg gagaactccg ccgcgacgac
     2221 gacgggcgcg tccgagccct cacgcagggt ctccatgaac gagtcgtagc ccccgccgtt
     2281 ctcgatgatc aggtcggcct tctgcacggc gagccgatcg cgggcggtgg cctcgtacga
     2341 gtgcgggtcc ttggctgcgt catcgatcag gctggtgacg tcggcgcggt cactggcgat
     2401 ctgccgcacg atgtcgccgt acacgttggt ggacgcgaca acctggaccc ggccgttgcc
     2461 ggacccgccg tccttcgatc cgtcggcggg accggccgcg caaccggcga ggacgagcag
     2521 ggatgaggcg gcgagcgccg cggagaccag gatctttcgc atgccctcat gctaatcgtt
     2581 aatgagaacc attatcaact tgacgttctc ggcgacttat tttccgccct gtcgcacttt
     2641 tattcttgct gtcagaatca aagtctgctc ccccgaagca gccccgacga tcagaggaga
     2701 ttgccatggc acccgtcgca cacgtgcttc tcgcccacgc gcatcccgac gacgagagct
     2761 gcgcgacagg cggcaccatc gcccgcctcc tcgccgaagg acacggcgtc accgtcgtca
     2821 cctcgaccca gggtgagcag ggccggatcg ccgtccccga gctgcagcat ctctctgtcg
     2881 acgaggacga tcagctcggc ccggagcgcg cgcgggagct cagcgacgcc atgaccgcgc
     2941 tcggcgtcac ggatcaccgc tacctgggcg gagcgggccg cttccgcgac tccggacgca
     3001 tgggcatccc cgcgaacgac cggtccgact ccttctggca ggcggacctc gacgaggcgg
     3061 gactgctcat ggcggagatc atccgtgaga ccaggccgac cgccgtcatc acctacgatc
     3121 cgaacggcgg ctacggccat ccggatcaca tccagaccca ccgcgtcacc atgcgcggca
     3181 tcgagctggc cgccgacgcg tcggccgcat ccggcctgcc ggtctgggac gtacccctcg
     3241 tgtactggac ggccgttccg cgcgagctga tcgtggcgga cctgaccgag ctggccgggc
     3301 acgccgagga gctcggtctc tgggtcgacc ccgaccccgc cgagtacccc gacggtgtgc
     3361 acgacgacga cgagatcgac gtcgagttcg atgtctccgc gcatctcgcc gcgaagaccg
     3421 ccgcactcgc ctgccaccgc acccagctca ccgtcaaggg cgactgctgg gtgctcgcct
     3481 cgggccgggg catgcgcatc cagaatcgcg agtggttcat ccgcgtgaag ggcgggcggc
     3541 gccggacccc cggatacgag accgagctgc tcccgcagca gccgaccacg gaaccggagg
     3601 ccgactgatg tcccgctcca ccctcgccct cgacgaccgc agcacgcagc gtgcgcgggt
     3661 ggtcggcgca ctgcacgaac agcgcaagag ccgctccgac ctggtcgagc agttcggagt
     3721 gagctcctcg ctgatcacca ggctcgtgcg cgggctgatc gacgacggca tcctgcgcga
     3781 ggtcggcgct caggctcagg ccatcgggcg tccgcccgtg cgcctcgagg tcgtccgcga
     3841 ccacggcgcc ctgctcgcgg tgtcctgcac ggccgagggg ctgcgtgcgc gctgcgtcaa
     3901 cctgcacggc gggatcatcg cggagaccag caccgccccg atcgcggggg tggtgagccc
     3961 ggagtcgatc gtcgagaccg tcgcgcagct gatcgctcgc acgccgcgcg tgctcggcat
     4021 cggcgtggcc gtcccgggcg tggtcgatgc ggagaccggc gacgtcagcg ccgcccctga
     4081 cctcggctgg accacggagg tgccgctgcg cacgatgctg cgggagcggt tcggcgtggt
     4141 cgtcacgatc gacaacgacg tgaacctcat gatggcctcc gagcggcgca ccctggacgg
     4201 tcgcgcagcc cgcaacgccg tctacctcta tctcggcgcc cgcggcatcg gcgcgggcat
     4261 catcgccgcg ggacgcctga tcgagggcga ccacggcgcg gccggcgaga tcggcctgtc
     4321 gccgttgagc gcgaacgacc gcaccgactt cgaggacacc acctccaccc tggccatcgc
     4381 ggcacggctg gaggccgcag ggcacaccgg atccgagccc gcggtggatc gactgctcgc
     4441 cctcgcggat gccggcgacc cggccgccgt gcgcatccgc gccgacgtgc tgacagcgtt
     4501 ctcccacgcg atcgccgtga tcaccgcgct cctcgacccg cacgtcatcc tgctgggcgg
     4561 ccatgcccgg gcgtttcgcg aggcggagca ccgcgagatg ctccagcccc tgcgcggcga
     4621 gctcccgttc ctcccggacg tgcgatccgc ccatccgaag ccggatgcca tcctcgacgc
     4681 cgcgctcaac cgctgctggc agcagcttct ggccggaggt ctatgagcat ccatcccacc
     4741 cgcaccgatc accaccccac ccgcggcacc cgccgcatca cttcgatgag gaagaacatg
     4801 aaggcacaca tcacgcgccg ccgtctggcg gcattcgcaa cattcgccac cgccggagcc
     4861 ctgctgctga gcggctgcgc cggcgacgtc gccccgccca agagcgaggc gcccaccgac
     4921 ggcggctcga aggtcacgcc cagcgagagc ctgacgatct acacgggtcg tgacaaggac
     4981 gaggtcgcct gggtcgtctc ggagttcgag aaggccaacc ccgactacgc gggcaaggtc
     5041 accacggtga tcgccggcgc ccaggataac ctcgaccgca tgcgcgccga ggccggcaac
     5101 ccgcaggccg gcttcctctg gggcggcacg cagcagcagt tcgagcaggc ggcgaaggag
     5161 ggcctgctgg ccgcctacga gccgaagaac ggcgacgcgg tcgacgcgaa gtacaaggac
     5221 ccgaagaacc agtggatcgc ggagatgctg ctgcccgagg tcatcatcta caactccgac
     5281 ctgctggacg agaagtccgc gccgcaggac tgggccgacc tcgccgagcc ggagtggaag
     5341 gacaagatcg tgatccgcga cgtcatgccc tcgggcacga tgcgcacgat ctacgcctcg
     5401 atggtctact cggctttcgc gaaggacagc aagcccgacg ccggctacga cctgctgaag
     5461 aagatcgacg cgaacacggt gtcctacgcc gcgaccccgg acgacatgta cacccagctc
     5521 gaccagggca ccggccaggt cacggtctgg aacatgcagg acgccctgat ccagccgctc
     5581 aagaacaacc ggccgtgggg ctacgtcatg ccgaagtcgg gcgctccggt gctggtggac
     5641 ggcgtcggca tcatcaagaa cgagaagcag caggccgccg cccaggcgtt catggacttc
     5701 ctgatggagc ccaagatgca ggccaagctc gccacggact actaccagat ccccgcgatg
     5761 gacatcccca aggcggacca gcccgactgg atgaaggact tcaagctcaa ggagatgaag
     5821 atcgactggg ccgtgtttgc ggagcaccag gacgagtgga tgacgtactg ggccgacaac
     5881 atcaaggaca agggctgatc tgacgcatgg tctcggtcac actgaaccag cttcgcaagt
     5941 cgttcgcgcg ctccggcacc gtcgtcgacg gcatcgacct gacgatcgcc gacggggagt
     6001 tcttcaccct gctcggaccg tcgggatgcg gaaagtcgac gacgctgcgg atgatcgccg
     6061 ggttcgaaga gcccgacgat ggcacgatcc acttcgacga ccgcgacgtg acgtacgcgc
     6121 cggcgaacaa gcgcgacgtg ggtctggtct tccagaacta cgcgctgttc ccgcacatgt
     6181 ccgtcacgcg caacgtcggc ttcgggctcg acgtgcgcaa ggtgcagaag gccgaggcct
     6241 cgcgtcgcat cagcgacgtg ctggcacagg tgggtctctc agctcaggcg ggcgcccgcg
     6301 tcgacgagct ctcgggcggg cagcagcagc gcgtcgcgct cgcccgggcg ctcgtcttcc
     6361 gtcctcagct gctgctgctg gacgagccga tgtccaacct cgacgccaag ctgcgtctgg
     6421 agatgcgcgg ggcgctgcgc gacctgcatg ccgagaccgg gatcacatcg gtctacgtca
     6481 cgcacgacca ggcggaggcg ctcgcgatgt cgacccgcat cgcggtgatg aacaccgccg
     6541 tcgtgcacca ggtcggcacg cccgacgagg tgtacagccg tccgcggacc gcgttcgtcg
     6601 ccggattcct cgggcgcaac aacctcctgc ctgcgaccgt tcgccgggtg gacggcgggc
     6661 gcgcgtctct cgagctcgcc gacggcagcg cgctggaggt gctcgtcgag aacgcggcgc
     6721 cgggcgtctc gttcgaaccc ggctccgccc tgatcgccac ggtccgcgca gagagcctgc
     6781 gcccggcggc atccggagag accgccaacg tcgtctccgg cacggtcgcc gacatcgact
     6841 tcgtcggata ctcgctgtcc tgcgagctcg acaccccgct cggccgcatc aaggtcgacc
     6901 tgaccggcac ccacgcccga ccgcagcgcg gcgacgccct gcgcgtcgtg ctgccggcgg
     6961 atgccgcgta ctgcgtcccg gcggagcagt gatgacgacg ctgacgaacg cgcagcgggt
     7021 caccggcccg tcgaagcgcc cgccgtggat ccggcgtcgc tggtccaccg tgctcacgct
     7081 catcctgctg atggtcgtgc tgctcggcta tgtgatcggg cccatgatcg ccaccgcccg
     7141 gcgcagcgtc tcccaggaca aggccgcggt gctgggcttc tcgttcaagg catggagcga
     7201 cttcttcgcc aatcccacgc agacgtccgc catcggcggg tcgatcgtgc tgtcgctgct
     7261 gtctgtgctg tgcgcgggcc tgctcgggac cctggtcgcg atcctgctca cccggtggga
     7321 gttccccggc cgccgcatct gccaggtgct ggcgctgctg ccgatcgcgc tgcctccgct
     7381 gatgggcgtg tggtcgttca agctgctctt cggcgtcggc gggcgcatcc cgttcgccat
     7441 gcaggagtgg ttcggcctgg accagaagat gttctggctg caggggctgg ccggcgtgct
     7501 catcgtgcac gccatgacga tgtaccccta cttcttcctg accgtctccg cggcgctctc
     7561 caacgccgac gcgtcgttgg aggaggcggc cgagtcgatg ggcgccggca gggcccgggt
     7621 ctgggccaag gtcgtgctgc cgatgctcac cccggcgctg gtcgccgggt cgctgatcac
     7681 gttcatgtca gcgatgtcgt cgtacacggc cccgctgctg ttccagtaca ccgacgtgat
     7741 gacgcagcag atcgtgatcg cgaagaccaa cggcgacatg cggctggcgt cgatcatctc
     7801 caccacgctc gcggtcatct ccatcgtgtt cctcgcgatc atccgctcct acgagcagcg
     7861 caaggtctac cgcacgcagt ccaagggcgg cggacgcaag cgctcgaccg tcacctccgc
     7921 cccggtgaag gtgctgctgt tcgtgatcgc cgttcccacg acggtgttcc tcgtgctgcc
     7981 gatcatcatg atcgcggtgc tctccttcac cgcgaagggc ggctggctgc gcagcgtgct
     8041 gcccaccggc tacaccctcg aatggtgggc ccagctcttc gacggtgccg agttctggcg
     8101 cccggtgacc aattccctgc tgatgagcgt gatcgccgtc ggcggcgcga tgatcctcgg
     8161 cgcgggtgcc gcgtacgtga tgagccgcat gcacttccgc ggcaagctcg cgctggacat
     8221 cgcgatcatg ctgccctggg cactgcccgg caccgtcgtc gcgatcaacc tgatcaccgc
     8281 gttctccagc ccgtcgccgt tcgcgttcgg tcaggtgctc gtgggcacgt tcgccatcct
     8341 tccgctggcg tacttcgtga ggttcaatcc gctgatcttc cggtccacgt tcgcgtcgtt
     8401 ctcgcagctc gacacgaacc tcgaggacgc tgcgcgcagc ctcggcgcca gctggtggta
     8461 cgccttccgc cgggtggtgc tgcccctgat ctcgcgcggc atcatggccg gtgccctgct
     8521 cgcgttcgtc gatggagtcg gcgagttcgt ggccagcatc ctgatctaca cccccgcctg
     8581 ggtgcccctg tcgatcgcga tcaacaacga gaactatcag ggcaacatcg gcaccggatc
     8641 ggtgtacggc atgatccagg tgctcctggt cctcggcgtg ctgatcgtca cccgccgcat
     8701 ggaggcgaag caggcgctcg cccccgccct ctgacacatc ccccggatgc cggcgacacg
     8761 tcggcgaccg tccatctgag agagaagaag aatgaaccgc gacgagatca tccagcgcga
     8821 tggagacgca ttccccggcg agagctacac cactgccgtg ctccgtcccg cctacgacca
     8881 ggcgaagcgg atgctgctgc cgtcgatgat cgagatccac cgtgcgcacc tggtgatgct
     8941 gcacgaggcg ggcatcctga acgacgcgga tgccgcggcg atcgcgcgct cgatcgacga
     9001 gctcgacctc gacgccctcg cggccagcga gtacaacggg cagttcgagg acctgttctt
     9061 caccgtcgag cacgagatga tgcgcatcgg cggcgaggtg accggcagcc tgcacacggc
     9121 ccgcagccgc aacgacatgg ggatgacgct gtaccgcatg gtcatgcgcc gtcagctgct
     9181 gcgggtgctg gatgccgccg gcgagctgta cgagacgctc gtgcgcctgg gcgcggagca
     9241 cgccgacacg ctgtcgttgg agcacaccca cacgcagccc gcgcagccgt cgatcctggg
     9301 gcatcgcttc ctcgcgatcg ccgacgtgct cggtcgcgac atccgccgcg tgcagcaggc
     9361 ctacgagagc ctggactact cgcccatggg cggggccgcc ctcacgggca ccggcttcgg
     9421 gatcgaccgc gaccacatgg cgcgcctgct gggcttccgc ggtctggtcg agaactcgta
     9481 cgacgcggtc gccgcgaccg attacatcgc gcactcggcg atcgccctgc agctcatggc
     9541 catcgacacc gggcgcagct gcgtggactt cctgacgtgg tgcacggccg agttcggcct
     9601 gtaccgggtg gccgccccct acgtgcagat ctcgtcgatc atgccgcaga agcgcaaccc
     9661 cgtgtcgatc gagcactccc ggtcgctgct gtcagccgca tccgcgagcg ccgcgacggt
     9721 cctgacgatg atgcacaaca cgcccttcgg cgacatcgtc gacaccgagg acgacctgca
     9781 gccgtacgca tggcgcgcgg tcgagatgct caccgacgtg ctgcagctgc tgaccggcgt
     9841 gctcggcacc atccaggtga acacggcggt gatgcgtgag cgcgcgctgt cctcgttcgc
     9901 gaacgcgacc gagctggccg acacgctggt gcgcgacggc ggcctgacct tcacgcaggc
     9961 gcacacggtc gtctcccgca tcgtgcgcga agcggatgcc gccggcatca ccgacatccg
    10021 ctcgatcgac ccggcgcgcg tgcacgaggt gacgaccgag gtcgccggac acgacgtgac
    10081 gctcacgccc gaggacctcg cgcgcgcact cgacgcggac aacttcgtgg ccgtgcgcac
//
LOCUS       DATMSX010000184        10772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135179,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000184 DATMSX010000000
VERSION     DATMSX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135179"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1535)
                     /locus_tag="VN041_05660"
     CDS             complement(63..1535)
                     /locus_tag="VN041_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWU28565.1"
                     /translation="MSVVLDLSAFVLVLVATVVLAVMGVRMSRTTSDFFVASRSARPV
                     WNASAISGEYLSAGTFLGLAGLVLLVGSDGFWFPIGYAAGYLLLLLFVAAPLRRSGAY
                     TIPDFVEARLESRTVRRVTSLLVLVIGWLYIVPQLHGASLTLGVVADLPPWVGAVVVA
                     VVVSGIVAAGGMRAITVVQAMHYWVKLSALAIPAVLMLIVLSGREPSSDPVVAFPAEL
                     GPVAADPYSTASLMIALLLGTIGLPHVLVRFYTSPDGASARRTTVLVVAMIGLFYAVS
                     GTLGLIARVAAPDLALPGVADTVVLALPARLLPEPLGTIVTAIVVAGAFAAFLATSSG
                     LVVSLAGVISQDLFDGSVRSFRLSAVLCVVAPLIVALLTGPEALVSSVGTVFLVAATS
                     LTPVILLGVWWRGLTARGALAGMLVGTLTVGASLIASIALDPQARAAASWIVQPAAWA
                     IPLVVVVIVLVSRWDRRRIPGRAEWYLTRLHLPERSGDPG"
     gene            complement(1532..1948)
                     /locus_tag="VN041_05665"
     CDS             complement(1532..1948)
                     /locus_tag="VN041_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal transporter"
                     /protein_id="HWU28566.1"
                     /translation="MSHGDGGRPARVRVSFQSPARSADAARASSGAAPAVRPVREAES
                     VFLTDLMRAQLRLAGACLIGFVLVSLTLTVAIMIIANTIDLPVFGVPLSWLLQAYAYY
                     PIILGFAVVFARNAARNERRFRMLADADSVQGGADG"
     gene            complement(1945..2673)
                     /locus_tag="VN041_05670"
     CDS             complement(1945..2673)
                     /locus_tag="VN041_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWU28567.1"
                     /translation="MIIDVLVADDEPPALAELVALLEADARIGAIHAASTGADALRML
                     SSEAVEAVFLDIHMPGVDGFDLARALGRFAQRPALVFVTADDAGAVTAFDLDALDYVL
                     KPVRPERLRRAIDRVFADARAKEAADDVDTGTVAVPVGQSVRLVRRADVRWVKAEGDY
                     SRLWTEAGDTHLVRIPISELEERWEAAGFVRIHRSYLVQADAITGVRLTGPLPVVELP
                     EVELPVSRRLIPAVRSRLVKVDSR"
     gene            complement(2670..3860)
                     /locus_tag="VN041_05675"
     CDS             complement(2670..3860)
                     /locus_tag="VN041_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU28568.1"
                     /translation="MTDQVLLSGGLGAAIGGLLTLVLLWARRFARASRDLGGEAERAT
                     SRTLHLAGLAAAELRADQGPELVRAARHLRNLLGVHAVAFVAPDGSVATDGAVTGLET
                     ASREIAARVQETGRRQVFPGTGAQDRTTLEAVGAPMTVDGESRGILIAYSDQVRGRLV
                     RATGQVAEWCAAQIALTELEASRASLAEAELRALRAQISPHFIYNSLAAIASFITTDP
                     ERARTLVLDFADFTRYSFRKKGEFATLAQELGSIRNYLELERARFGDRLDVQLRIAPE
                     TLSTVIPFLSVQPLVENAVRHGLEPLASGGRIIIAAADESTHTEVSVEDDGAGMDPAA
                     VRRALAEESAQGTHVGLRNVDTRLRRLYGPEGGLIIETNLDAGTLVRMRIPKSQPLHE
                     TEER"
     gene            complement(3893..4129)
                     /locus_tag="VN041_05680"
     CDS             complement(3893..4129)
                     /locus_tag="VN041_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HWU28569.1"
                     /translation="MAVSPLDAVPAPVAFWRRVRGWASSIRWYLTTLMGDRAYDVYVG
                     HCRAQHPDAAIPTEREFWRDRMAAQDANPGARCC"
     gene            complement(4129..6381)
                     /locus_tag="VN041_05685"
     CDS             complement(4129..6381)
                     /locus_tag="VN041_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWU28570.1"
                     /translation="MTTPSAAGDGTHSAAQALDTPPTAVAEEDRRRDSRWTLPKILIW
                     VAIALLGAVAWTMLAFVRGETVNAIWFVFAAVCTYLIGYRFYSKVIERHLLRPDDRRA
                     TPAEIREDGKDYVPTDRRVLYGHHFAAIAGAGPLVGPVLAAQMGFLPGTIWIIVGVVL
                     AGAVQDYTVLFFSMRRGGRSIGQMARDVLGRIGGTAAIIASLLIMIIIIAILALVVVN
                     ALGESPWGVFSVAMTLPIALFMGLYLRFLRPGRITEVSLIGFVLLIAAIVGGGAVAGT
                     EWGQAMFHLDRSVIAWGIIVYGFIAAILPVWLLLAPRDYLSTFMKIGVIVMLAGAIVL
                     VRPEITVPAFTDFAFTGLGPVFSGPLFPFLFVTIACGALSGFHALISSGTTPKLVEKE
                     RQTRFIGYGGMLMESFVAIMALVAAISIDQGIYFAMNAPAALTGGTAESAVAFVNSLG
                     LTGPSLTPDVLTSTAAAVGEESIISRTGGAPTLALGLAHIMHQIGGGTAMMGFWYHFA
                     IMFEALFILTAVDAGTRVARFMLQDSIGAWVPRFRDNSWRPGVWICTAIMVAGWGYIL
                     ILGVTDPLGGINTFFPLFGIANQLLAAIALAVVFAIVAKRGRDYVRWLWIIGVPLVFT
                     AVITITASMYKIFSPVPSIGYWANNAKYRAAQAAGDDALGAPEVIEAVIRNTAVQGTL
                     SIVFVVLTIIVIIVAAVVAISAIRRGAEDDGEDAPQPSRRFAPAGLITTRAERELEAQ
                     WRDVGAGQGR"
     gene            complement(6493..7887)
                     /locus_tag="VN041_05690"
     CDS             complement(6493..7887)
                     /locus_tag="VN041_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU28571.1"
                     /translation="METNDTQISGFSRRGALRLFGAGVGAAGLGALGLAQPAAAAPLP
                     AVVPAAGDAGFWRKVEQMFVLDKRILYMNVGTVGSPPRQVLDVVDAVHRQVARDAEAS
                     YSDFADVRQVIATAHGVDKDEIVITHNTSDGMSKALLGLNLREGDEILTTNHEHGGGN
                     TPMALLQDRQGVVIKRVELPIGDKQTAEDYVELFRAGITSRTKLIMFSAPTYLTGTML
                     PIRELAVLAQEHGITTLVDGAHIPGMMNYDFRELGVDFLAASAAKWQCAPGGTGILYI
                     RNRITAANPNPLPEFWPIVSSGYPTNGSVDLRHRDPSQDIGKYLQSVGNGTKALNAGV
                     QKAVEVWNEIGRQRIHDHVVGLGDYAKEQIVERWGVDALYSAKSDQRLTCALTGFNPF
                     QNPADVKDANKSSEFVARLLDEYRVEIRNTAVPVIGDSVLHRPVRVSTHLFHQQEHVD
                     RMIDAAWKLSKRIA"
     gene            complement(7900..8424)
                     /locus_tag="VN041_05695"
     CDS             complement(7900..8424)
                     /locus_tag="VN041_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWU28572.1"
                     /translation="MFNFAPRSRRAALLVAGTILVTGLASTSLLAGAAQGASSPSDHS
                     SSSSKRVLHTTDAPAAIGPYSQGIAAGRTVYVSGQLPIDPTVGKIDNSQSIAEQTALS
                     VKNIRAVLKEARLTLDDVVSTTVYLADMNDFAAFNTVYAEYFGTESAPARATVQVAAL
                     PQGAKVEISAIAVH"
     gene            complement(8631..>10772)
                     /locus_tag="VN041_05700"
     CDS             complement(8631..>10772)
                     /locus_tag="VN041_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HWU28573.1"
                     /translation="DELGLSATTSEEFAAVLASAHEAEGEGSSRRAARAAFADAETAR
                     RSLMRRRGELDAERQKADAVHGSIPSPLHEARMLLARAAGLDAEDLPFVGELIEVRTE
                     FEPWREAFNLALGGFATTLLIDAENVSAFRAAIDQVRTPIRLRYEGVQAGLATSAGAD
                     GRSLPGRLDFRPTPFTGWLQQRLDERFGFVCVDSSAQLSAHSMALTITGQMSQGNRGA
                     HGGHGRDNVLGFSNERRLHDLERQLEQLDAQIVAATDAADAAEQAMDAIENRLTAFAK
                     IQDVTWEQIDVDALAQEVERWASVEAEVTGANPEIALLKEQAETLKLKASVLRDEIGS
                     LSAQRDQVAERWGDVTDDVDDCQRVIDAAEGMELDAGQTAFLDDRFILADESDDAASD
                     RLARLDVALKTAAARLAGDRRAASEAYEEQRERMRQLMASFLERWPNLNLLPDPDESV
                     AEFEGILEALKTSGLHELESEWRDSLLGLSGNDLTSLDSTLSRSVREIRERIEPINSI
                     MRELPFYDDDHRLQISPRENQSEARKRFRRDLREVRGLIEAASTDEERENAYRRMSRL
                     IGRMRRTAPDFAELIDVRNHVRVSAERVVAATGEHVALYDHIGEKSGGESQELVAFIV
                     GAALRYQLGDAGAERPRYAPVFMDEALIKADAHFTKRAIGAWRGLGFQLVIGAPNDKY
                     SAIEPHVDVEYTILKDTRGRSWAKPKVAVEG"
BASE COUNT         1713 a         3779 c         3742 g         1538 t
ORIGIN      
        1 atcacgcccg agcccgtcca gtaacagggc gtgccggaga cccgccgaag tacgagcgac
       61 tctcagccgg ggtcgccgct gcgctccggc aggtgcaggc gtgtgagata ccactccgcg
      121 cggcccggga tccgccgccg gtcccaccgc gagaccagca cgatcaccac gacgaccagc
      181 gggatcgccc acgccgcggg ctgcacgatc caggaagccg cggcccgcgc ctgcgggtcg
      241 agggcgatcg acgcgatcag cgacgcgccc accgtgagcg tacccaccag catccccgcg
      301 agcgcccctc gggcggtcag accgcgccac cacacgccga gcaggatgac cggagtcagg
      361 ctggtcgccg ccaccaggaa caccgtgccg acgctcgaca ccagggcttc cggtccggtc
      421 agcagcgcca cgatcagtgg tgcgacgacg cacagcacgg ccgaaagacg gaatgaccgc
      481 accgacccgt cgaagaggtc ctggctgatc acgcccgcaa gagagaccac cagacctgac
      541 gacgtcgcca ggaacgccgc gaaggcgccg gcgacgacga tcgccgtgac gatggtgccg
      601 agcggctcgg gcagcagccg ggcgggcagc gccagcacga ccgtgtcggc cacccccggg
      661 agcgccagat cgggtgcggc gacgcgcgcg atcagcccga gcgtgcccga caccgcgtag
      721 aacaggccga tcatcgcgac gacgagcacc gtcgtccgcc gcgcagaggc cccgtccggg
      781 ctggtgtaga accgcaccag cacgtgcggg agaccgatcg tgcccagcag cagcgcgatc
      841 atgagggatg ccgtcgaata gggatccgcc gccaccggcc ccagctccgc ggggaaggcg
      901 accaccgggt cggacgacgg ctcccgcccc gacagcacga tcagcatgag caccgccgga
      961 atggccagcg cgctgagctt gacccagtag tgcatggcct gcacgaccgt gatcgcgcgc
     1021 atgccaccgg ccgccacgat gcccgacacg acaacagcga cgacgacggc accgacccag
     1081 ggcggcagat ccgccacgac gcccagcgtc agtgacgccc cgtgcagctg cggaacgatg
     1141 tacagccagc cgatcaccag caccagcagg ctcgtgaccc ggcgcacggt gcgggactcc
     1201 agccgagcct ccacgaagtc cgggatcgtg tacgcgccgc tgcgtcgcag cggcgcagcg
     1261 acgaacagca gcagcagaag atatcccgcc gcgtacccga tcgggaacca gaacccgtcc
     1321 gaaccgacca gaagcacgag cccggccagc cccaggaacg tgcccgcgct gagatactcg
     1381 ccactgatcg cggaggcgtt ccacaccggg cgggcgctgc gggacgccac gaagaagtcg
     1441 ctcgtggtgc ggctcatccg cacgcccatc accgccagca cgacggtggc cacgagcacg
     1501 agcacgaacg ccgagagatc gagaacgacg ctcatccgtc ggcaccgccc tgcacggagt
     1561 ccgcgtccgc gagcatccgg aatcggcgct cgttgcgcgc cgcgttgcgg gcgaaaacca
     1621 ccgcgaagcc caggatgatc ggatagtacg catacgcctg cagcagccac gacagcggca
     1681 ccccgaagac cggcaggtcg atcgtgttcg cgatgatcat gatcgcgacg gtcagcgtga
     1741 gcgagaccag cacgaacccg atcaggcagg cgccggccag gcgcagctgt gcgcgcatca
     1801 gatcggtgag gaacaccgac tcggcctcgc ggaccggccg cactgcgggt gcggcgccgc
     1861 tggacgcccg cgctgcgtcg gcagaacggg ccggagactg gaatgagacc cgaacgcgcg
     1921 ccggacgacc gccgtcgccg tgactcatcg gctgtccacc ttcacgagtc ggctccgcac
     1981 cgcggggatg aggcgccggc tgacgggcag ctcgacctcg ggcagctcca ccaccggcag
     2041 agggccggtc agccgcactc ccgtgatcgc atcggcctgg acgaggtacg agcggtggat
     2101 gcgcacgaac cccgcggcct cccagcgctc ctcgagctcg gagatcggga tgcgcacgag
     2161 gtgcgtgtcg cccgcctccg tccacagccg cgagtagtcg ccctccgcct tcacccagcg
     2221 cacgtcggcg cggcgcacca gccgtacgga ctgcccgacc ggaacggcga ccgtcccggt
     2281 gtcgacgtcg tcggcggcct ccttcgcccg tgcgtcggcg aacacccggt cgatcgcgcg
     2341 acgcaggcgc tcgggacgga cgggcttgag cacgtagtcg agcgcgtcca ggtcgaaggc
     2401 cgtcacagct ccggcgtcgt ccgcggtgac gaacacaagg gccggtcgct gcgcgaaccg
     2461 ccccagcgcc cgcgcgagat cgaacccgtc gactccgggc atgtggatgt cgaggaagac
     2521 ggcctcgacg gcctcgctcg agagcatccg cagcgcatcg gcgccggtgg acgccgcgtg
     2581 gatggcgccg atcctggcat ccgcctccag cagcgcgacg agctcggcga gcgccggcgg
     2641 ctcgtcgtcc gcgaccagca cgtcgatgat catcgctcct ccgtctcgtg cagcggctgc
     2701 gacttcggga tccgcatccg caccagcgtc cccgcgtcga ggttcgtctc gatgatcagc
     2761 ccgccctcgg gtccgtacag ccggcgcaga cgcgtgtcga cgttgcgcag cccgacgtgc
     2821 gtgccctgcg cgctctcctc ggcgagcgcg cgccggaccg cagccggatc catgcccgct
     2881 ccgtcgtcct cgaccgagac ctcggtgtgc gtgctctcgt cggcggcggc gatgatgatc
     2941 cggccaccgg atgcgagagg ctcgagcccg tgccggaccg cgttctcgac cagcggctgc
     3001 accgacagga aggggatcac ggtcgagagg gtctcggggg cgattcgcag ctgcacgtcc
     3061 agcctgtccc cgaaccgcgc acgctccagt tcgaggtagt tgcggatgct gcccagttcc
     3121 tgcgcgaggg tggcgaactc gcccttcttg cggaacgagt accgggtgaa gtcggcgaag
     3181 tcgagcacga gcgttctggc ccgctcggga tccgtggtga tgaacgaggc gatcgcggcc
     3241 agcgagttgt agatgaaatg cgggctgatc tgcgcgcgca gggcccgcag ctcggcctcg
     3301 gcgaggctgg cacgcgaggc ctccagctcc gtcagcgcga tctgagcggc gcaccactcc
     3361 gcgacctggc cggtggcgcg cacgagccgt ccgcgcacct ggtcggagta ggcgatcagg
     3421 atgccgcggc tctcgccgtc cacggtcatc ggtgcgccca ccgcctcgag ggtcgtccga
     3481 tcctgggctc cggtcccggg gaacacctgc ctgcggccgg tctcctgcac gcgtgcggcg
     3541 atctcccgcg atgccgtctc cagtcctgtg acggcgccgt cggtggcgac ggagccgtcc
     3601 ggcgccacga acgcgacggc gtgcacaccg agaagattcc gcagatgccg ggctgcgcgc
     3661 acgagctcag ggccctgatc agcgcgaagc tcggcggcgg cgaggccggc gaggtggagc
     3721 gtccggctcg tggcccgctc ggcctctccg ccgagatctc gggatgctcg cgcgaagcgt
     3781 cgcgcccaca gcagtacgag tgtcagcagc cccccgatcg ccgctcccag cccgcccgag
     3841 agcagtacct ggtcggtcat gccttcacca tacggtcgcc agcggggtcc tgtcagcagc
     3901 accgcgcccc ggggttcgca tcctgcgccg ccatccggtc ccgccagaac tcgcgttcgg
     3961 tggggatcgc ggcatccgga tgctgtgccc ggcagtgccc gacgtagacg tcgtacgcac
     4021 ggtcgcccat cagagtcgtg agataccagc ggatgctgga ggcccagccc cgcacccgcc
     4081 gccagaaggc gacgggtgcg gggacggcgt ccaacgggga cacggccatc agcggccctg
     4141 cccggcgccg acgtcgcgcc actgtgcttc gagctcgcgc tcggcgcgcg tggtgatcag
     4201 gcccgccggc gcgaagcggc gggacggctg aggagcgtcc tcgccgtcgt cttcggcacc
     4261 acggcggatc gccgagatcg cgacgaccgc agcgacgatg atcacgatga tcgtgagcac
     4321 gacgaacacg atcgacagcg tgccctgcac cgcggtgttg cggatcacgg cctcgatcac
     4381 ctcaggggcg cccagcgcgt cgtcaccggc ggcctgcgcg gctcggtact tcgcgttgtt
     4441 ggcccagtac ccgatgctcg gcaccggcga gaagatcttg tacatcgagg ccgtgatggt
     4501 gatcaccgcg gtgaagacca gcggcacgcc gatgatccac agccagcgca cgtagtcccg
     4561 cccgcgcttg gcgacgatgg cgaacaccac ggcgagcgcg atcgcggcca gcagctggtt
     4621 ggcgatgccg aacagcggga agaacgtgtt gatgccgccc agcggatcgg tgactcccag
     4681 gatcaggatg tacccccacc ccgcgaccat gatcgcggtg cagatccaga cgcccgggcg
     4741 ccagctgttg tcgcggaacc tcggcaccca ggcgccgatc gagtcctgca gcatgaagcg
     4801 cgcgacacgg gtgccggcgt cgacggcggt gaggatgaac agcgcctcga acatgatggc
     4861 gaagtggtac cagaagccca tcatcgccgt gccgccgccg atctggtgca tgatgtgcgc
     4921 caatccgagc gcgagcgtcg gcgccccgcc ggtgcgggag atgatgctct cctcgcccac
     4981 ggccgcggcc gtcgacgtga gcacgtccgg ggtcaggctc ggaccggtca ggcccagcga
     5041 gttgacgaag gccaccgcgc tctcggcggt gccgccggtg agtgcggccg gcgcgttcat
     5101 cgcgaagtag atcccctggt cgatcgagat cgcggccacc agcgccatga tcgccacgaa
     5161 cgactccatc agcatcccgc cgtagccgat gaagcgggtc tgtcgctcct tctccaccag
     5221 tttcggagtg gtgccggagg agatcagcgc atggaagccg gacagcgcac cgcacgcgat
     5281 cgtgacgaag aggaacggga acagcggtcc ggagaacacc ggcccgagcc cggtgaacgc
     5341 gaagtccgtg aaggcgggga ccgtgatctc cggacggacc aggacgatcg ctcccgcgag
     5401 catcacgatc acgccgatct tcatgaacgt ggacaggtag tcgcggggcg cgagcagcag
     5461 ccacaccggg aggatcgcgg cgatgaagcc gtagacgatg atgccccacg cgatcacgga
     5521 gcggtccagg tggaacatcg cctgtcccca ctcggtgccg gccaccgcgc ctccgccgac
     5581 gatcgcggcg atcagcagca cgaagccgat cagcgacacc tcggtgatgc gccccgggcg
     5641 caggaaccgc aggtagaggc ccatgaagag cgcgatgggc agcgtcatcg cgacgctgaa
     5701 cacgccccac gggctctcac cgagagcgtt gacgaccacc agcgcgagga tcgcgatgat
     5761 gatgatcatg atgagcagcg aggcgatgat ggcggccgtt ccgccgatcc tgccgagcac
     5821 gtcgcgcgcc atctgtccga tggagcggcc accgcgccgc atggagaaga acagcacggt
     5881 gtagtcctgc accgcgccgg cgagcacgac gccgacgatg atccagatcg tgccgggcag
     5941 gaatcccatc tgcgcggcga gcacggggcc gaccaggggg cccgccccgg cgatggcggc
     6001 gaagtggtgg ccgtagagca cccggcggtc ggtcgggacg tagtccttgc cgtcctcccg
     6061 gatctcggcg ggggtggcgc gacggtcatc gggtcgcagc aggtgcctct cgatcacctt
     6121 ggagtagaag cggtagccga tcaggtacgt gcacaccgcg gcgaacacga accagatggc
     6181 gttgacggtc tcgccgcgca cgaacgcgag catcgtccag gcgacggcgc cgagcagggc
     6241 gatcgccacc cagatcagga tcttgggaag agtccagcgg gagtcccgcc gccggtcctc
     6301 ctcggcgacg gctgtgggcg gtgtgtcgag cgcctgtgcg gcggagtggg ttccgtcgcc
     6361 ggctgcggaa ggtgtggtca tggtctcctc cttgagacgc gggcaggtcg ggtgcgctgc
     6421 gagtgcgcga ggtcgggcga cgatcacggc cggctcatcc gctgggcgga tgagccggcc
     6481 gcgtcgccga ggtcaggcaa tccgcttgga gagcttccag gcggcgtcga tcatgcggtc
     6541 gacgtgctcc tgctgatgga acaggtgcgt cgagacgcgc acgggccggt gcaggacgga
     6601 atcgccgatg accggcaccg cggtgttgcg gatctcgacc cggtactcgt ccaggagccg
     6661 cgcgacgaac tcggacgact tgttcgcgtc cttcacatcg gccgggttct gaaacgggtt
     6721 gaacccggtc agtgcgcagg tgagacgctg gtcggacttc gccgagtaca gcgcgtcgac
     6781 cccccaccgc tcgacgatct gctccttcgc gtagtcgccg aggcccacga cgtgatcgtg
     6841 gatgcgctgg cgcccgatct cgttccacac ctcgaccgcc ttctgtacgc cggcgttgag
     6901 ggccttggtg ccgttgccga cggactggag gtacttgccg atgtcctgcg aggggtcgcg
     6961 gtgccgcagg tcgaccgacc cgttcgtcgg gtaccccgac gacacgatcg gccagaactc
     7021 cgggagcgga ttcgggttcg cggccgtgat gcggttgcgg atgtagagga tgccggtgcc
     7081 gccgggtgcg cactgccact tcgcggcgga tgccgcgagg aagtcgaccc cgagctcgcg
     7141 gaagtcgtag ttcatcatcc cggggatgtg cgcgccgtcg acgagggtcg tgatcccgtg
     7201 ctcctgcgcc agcaccgcca gctccctgat cggcagcatc gtgccggtga ggtaggtggg
     7261 cgcggagaac atgatcagct tggtgcggct ggtgatgccg gcgcggaaga gctccacgta
     7321 gtcctcggcg gtctgcttgt caccgatcgg cagctccacc cgcttgatga cgacgccctg
     7381 gcggtcctgc agcagggcca tcggggtgtt gccgccgccg tgctcgtggt tggtcgtcag
     7441 gatctcgtcg ccctcgcgca ggttcagccc gagcagtgcc ttggacatgc cgtccgaggt
     7501 gttgtgcgtg atgacgatct catccttgtc gacgccgtgc gcggtggcga tcacctggcg
     7561 cacgtccgcg aaatcgctgt acgaggcctc ggcatcgcgg gcgacctggc ggtgcacggc
     7621 atccacgacg tccagcacct gacgcggcgg cgagccgacc gtgccgacgt tcatgtagag
     7681 gatcctcttg tcgagcacga acatctgctc gaccttcctc cagaatcccg cgtcgccggc
     7741 agccgggacg accgcgggaa gcggcgcggc ggcagcaggc tgcgcgagtc cgagcgcgcc
     7801 gaggcccgct gcgccgacgc cggcgccgaa cagcctgagg gcaccgcggc gtgagaaacc
     7861 gctgatctgc gtgtcattcg tttccatggt gttcatctcc tagtggacgg cgatcgcact
     7921 gatctcgacc tttgcgccct ggggcagcgc ggcgacctgg acggtcgcgc gagccggcgc
     7981 ggattcggtg ccgaagtact cggcgtagac cgtgttgaag gcggcgaagt cgttcatgtc
     8041 tgcgaggtac acggtcgtgc tcacgacgtc gtcgagcgtc agcctcgcct ccttgagcac
     8101 cgccctgatg ttcttcaccg acagcgcggt ctgctcggcg atcgactggg agttgtcgat
     8161 cttgccgacg gtcgggtcga tgggcagctg ccccgacacg tagaccgtcc tgcctgcggc
     8221 gatcccctgc gagtaggggc cgatggcggc cggcgcgtcg gtggtgtgca gcacccgctt
     8281 cgaggagctg gaggaatgat cggatgggga cgatgcaccc tgcgcggcac cggccagcag
     8341 gctggtggac gcgaggcccg tgaccaggat cgtcccggcg accaggaggg ccgctcttcg
     8401 ggaccggggg gcgaagttga acatctactc tccttcgagt cagacgagca cccggcgttc
     8461 tgtgctcatg cgcgtcatcg cgtcctgtca cggtagggag caacgtcgtc gggcgggaga
     8521 gtcgccgacc gactgtgcgc gttcatgcga cgaacggtgc gatcggcgcg accaacggca
     8581 gaggcccggg ggcgctgcac tccccgggcc tcccctgtcg tcgcttcgcg tcagccctcg
     8641 acggccacct tcggcttggc ccaggacctg ccgcgggtgt ccttgaggat cgtgtactcc
     8701 acgtccacgt gcggctcgat cgcgctgtac ttgtcgttcg gggcgccgat caccagctgg
     8761 aagcccaggc cccgccaggc gccgatcgcg cgcttggtga aatgcgcgtc ggccttgatg
     8821 agggcctcgt ccatgaacac cggcgcgtag cgcgggcgct cggcaccggc atcccccagc
     8881 tggtagcgca gcgcggcgcc gacgatgaac gcgaccagct cctgcgactc accaccggac
     8941 ttctcgccga tgtggtcgta cagggcgacg tgctcgcccg tcgccgcgac gacgcgctcc
     9001 gccgacaccc gcacgtggtt gcgcacgtcg atcagctcgg cgaagtcggg cgcagtgcgg
     9061 cgcatgcgac ctatcagcct gctcatgcgg cggtatgcgt tctcgcgctc ctcgtcggtg
     9121 gaggccgctt cgatcagccc gcgcacctcg cgcaggtcgc ggcggaagcg cttgcgcgct
     9181 tcggactggt tctcccgcgg cgagatctgc agccggtgat cgtcgtcgta gaacggcagt
     9241 tcgcgcatga tgctgttgat cggctcgatg cgctccctga tctcgcgcac cgaccggctc
     9301 agcgtcgagt ccaggctggt cagatcgttg ccggacagac cgagcaggct gtcgcgccat
     9361 tcgctctcca gctcgtgcag cccgctggtc ttcagcgcct ccaggatgcc ctcgaattcg
     9421 gcgaccgact cgtcggggtc gggcagcagg ttgaggttcg gccagcgctc caggaaggac
     9481 gccatcagct gccgcatccg ctcgcgctgc tcctcgtacg cctcggatgc tgcgcgccga
     9541 tcgcccgcga gccgcgccgc ggccgtcttc agtgcgacgt cgagtcgcgc gaggcggtcg
     9601 ctggcggcat cgtcggactc atcggcgagg atgaaccggt cgtcgaggaa cgcggtctgg
     9661 ccggcatcca gctccatgcc ctccgcggca tcgatcacgc gctggcagtc gtccacgtcg
     9721 tcggtcacat cgccccagcg ctcggcgacc tggtcgcgct gcgcgctcag cgaaccgatc
     9781 tcgtcgcgca gcacggatgc cttgagcttg agcgtctcgg cctgctcctt cagcagcgcg
     9841 atctcggggt tcgccccggt gacctcggcc tcgacggatg cccagcgctc cacctcctga
     9901 gccagggcgt cgacgtcgat ctgctcccag gtcacatcct ggatcttcgc gaacgcggtg
     9961 agccggttct cgatggcgtc catcgcctgc tcggccgcat ccgcggcatc cgtcgccgcg
    10021 acgatctgcg catccagctg ctccagctgc cgctcgaggt cgtgcaggcg gcgctcgttc
    10081 gagaacccga gcacgttgtc gcggccgtga ccgccgtgcg ccccgcggtt gccctgggac
    10141 atctgaccgg tgatggtgag agccatgctg tgggcggaga gctgtgcgga ggagtccacg
    10201 cagacgaagc cgaagcgctc gtcgaggcgc tgctgcagcc agcccgtgaa cggcgtcggg
    10261 cggaagtcga gccgtccggg aagactgcgc ccatcagcgc cggccgacgt cgcgaggccg
    10321 gcctgcacgc cctcgtagcg cagccggatc ggcgtgcgca cctggtcgat cgctgcccgg
    10381 aacgcggaca cgttctccgc gtcgatgagc agcgtcgtcg cgaatccgcc gagcgccagg
    10441 ttgaacgcct cgcgccaggg ctcgaactcc gtgcggacct cgatcagctc gccgacgaac
    10501 ggcaggtctt cggcatcgag cccggccgcg cgggccagca gcatccgggc ctcgtgcaaa
    10561 ggcgagggga tgctgccgtg cacggcatcc gccttctgcc gctcggcgtc gagctcgcca
    10621 cgacgacgca tcagcgaacg ccgcgcggtc tcggcatccg cgaacgccgc gcgcgcggcg
    10681 cggcgggagg agccctcgcc ctcggcctcg tgcgcggacg cgagcaccgc cgcgaactcc
    10741 tcggacgtcg tggccgacag accgagctcg tc
//
LOCUS       DATMSX010000185         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135181,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000185 DATMSX010000000
VERSION     DATMSX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135181"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN041_05705"
     CDS             <1..349
                     /locus_tag="VN041_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28574.1"
                     /translation="GCAARVAGDEEGAQLEFDAARSVLEQLGAVPDLARLERLAATVH
                     PASAEPGLSRREREVLELVAQGCSNRGIAERLFLSERTVARHVGNILSKLDVPSRSAA
                     TAYAYAHGLVSAI"
     gene            472..1563
                     /locus_tag="VN041_05710"
     CDS             472..1563
                     /locus_tag="VN041_05710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU28575.1"
                     /translation="MSTTALDLAIIGAGAAGLHTARRAMDRGVRLEVFDASARIGDSW
                     RERYRSLRLFTPRRWAELPGLRLDIGMFEYPTAAQLADYLERYVTAFEIPVRTGIRVQ
                     RLSTAPNGLFHLELSTGEDVVADKVVVAAGAHHRPLLPGCAVDVDPAIRQVHSLEYRG
                     PEDLATGSVLVVGAANSGTDIALEAAANGHETVLAGRNPDQVPGRIDTPVGNLISSIF
                     IRRLRNLTLDTEKGRRAWELQRGHGVNLVRNHLSDLRRAGIRQAGRLEGVQGGLPVLA
                     GGERLRPATIVWCTGSEPIRDWIDLPGYADEAGIHHDRGLSTTVPGLAFVGLPFQYSI
                     ASPTLMGMGRDAEYVVEHLLQPAPVLRAA"
BASE COUNT          235 a          592 c          574 g          250 t
ORIGIN      
        1 cggctgcgcc gcccgggtcg ccggagacga ggagggcgcc cagctcgagt tcgacgcggc
       61 ccgctcggtg ctggagcagt tgggtgccgt gccggacctc gcacggctgg agcggctcgc
      121 cgctaccgtt cacccggcat ccgccgagcc gggcctgagc cgtcgcgaac gggaggtgct
      181 cgagctcgtc gcgcaggggt gctcgaaccg cgggatcgcg gaacgtctct tcctcagcga
      241 gcgcacggtc gcccggcatg tcgggaacat cctcagcaag ctcgacgtgc cgtcgcgctc
      301 ggcggcgacc gcctatgcct acgcacacgg gctggtcagc gcgatctgac cctcgccgac
      361 catcggctcc atgacctgtc gaattggtca tttctgccga tgcccgcctg gcgaccgccg
      421 acctactgtt ctcagacgcg gggaatccga atccccggtg aggaggcgat catgtccacc
      481 acggcactcg atctggccat catcggcgca ggtgcggcag ggctgcacac agcacgccgt
      541 gcgatggatc gaggcgtgag gctcgaggtg ttcgacgcga gcgcgcgcat cggcgactcc
      601 tggcgcgagc gctatcgctc tctgcggttg ttcaccccga ggcgctgggc cgagctgccc
      661 gggctccgcc tcgacatcgg gatgttcgag taccccaccg ccgcgcagct cgccgactat
      721 ctggaacggt acgtcaccgc gttcgagatc cccgtccgca cgggcatccg cgtgcaacgc
      781 ctctcgacgg cgccgaacgg actgttccat ctcgaactca gcaccgggga ggatgtcgtc
      841 gcggacaagg tggtcgtggc cgcgggtgcg caccaccgcc cgctcctacc cggctgcgcc
      901 gtcgatgtgg atccggcgat acgtcaggtg cactcgctcg agtaccgcgg acccgaggat
      961 ctcgccacgg gatccgtgct ggtcgtgggc gcggccaact cggggaccga catcgccctc
     1021 gaggctgcgg cgaacggtca cgagacggtg ctcgcggggc gcaatccgga ccaggtgccc
     1081 gggcggatcg acacaccggt cggcaacctg atcagcagca tcttcatccg gcggctgcgc
     1141 aatctcaccc tcgacacgga gaagggccga cgcgcatggg agctgcagcg cggacacggc
     1201 gtgaacctcg tccgcaatca tctctccgat ctgcgccggg cgggcatccg ccaggcgggc
     1261 cggctggagg gcgtccaggg cgggctcccg gtcctggccg gcggcgagcg cctgcgcccc
     1321 gcgacgatcg tctggtgcac cggatccgag ccgatccggg actggatcga tcttcccggc
     1381 tacgccgacg aagcgggtat ccaccacgac cgcggtctgt cgacgaccgt gcccggtctg
     1441 gccttcgtgg gcctgccctt ccagtactcg atcgcctcgc ccacgctgat gggcatgggg
     1501 cgcgatgccg agtacgtggt cgagcacctg ttgcagcccg ctcctgtgct cagggccgca
     1561 tgaggcctgc gagccgcacg agtggcggcg gccgggccgc tcccggccac tgcgctcagc
     1621 gcgccggcag gatcaccgtc ggcgtgggcg c
//
LOCUS       DATMSX010000186         5454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_69081,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000186 DATMSX010000000
VERSION     DATMSX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69081"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN041_05715"
     CDS             complement(<1..249)
                     /locus_tag="VN041_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28576.1"
                     /translation="MTTTAPLPTVTETVRRMPYAALLTMMAMSFLLVSAEFLPNGVLT
                     EIAAELGVTPGQAGQLVTVTALAGLIVAPTIGLALPRLD"
     gene            complement(360..1058)
                     /locus_tag="VN041_05720"
     CDS             complement(360..1058)
                     /locus_tag="VN041_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28577.1"
                     /translation="MADPDEGAQEPDATTVGGAEGAAPPSGDGSGAGGLTEPGTEATG
                     TPVPDAVATDADVPELQSPGAGAAGAAVADADVPDLEIPDAETFVPDLEIPDADVPEL
                     DQREPVIPDPVLPESFLTEPVIPPPPPDDPPVARPAWRSDRDYPAGSGTRVPEGAPSV
                     TRATRRAASDSLGSPSAPAPDPRLTAPDPDPAVVPPPSGATSGGYRGLTIAIYGVLGA
                     LLVGAVVMISTLLR"
     gene            complement(1060..1665)
                     /gene="dcd"
                     /locus_tag="VN041_05725"
     CDS             complement(1060..1665)
                     /gene="dcd"
                     /locus_tag="VN041_05725"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWU28578.1"
                     /translation="MLLSDRDIKAELASGRIGLSPYEEEMVQPSSVDVRLDRYFRLFD
                     NHKYPFIDPSVEQPELTRLIEVAPDEPFILHPGEFALGATFEQVSLPDDVAARLEGKS
                     SLGRLGLITHSTAGFVDPGFTGHVTLELANVATLPIKLWPGMKIGQFCFFRLTSPAEN
                     AYGSGPYGNRYQGQRGPTASRSFLNFHRTDVGTTDAGAIGG"
     gene            1828..2394
                     /locus_tag="VN041_05730"
     CDS             1828..2394
                     /locus_tag="VN041_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HWU28579.1"
                     /translation="MNDSITAQTETPIRSRKRLIVPLAGLLIAAAITVGSGADFVANS
                     VNAANAFSTGTLTQSNSKSGSAIFNAANLKPGDTLNGTVTITNSGSLPADFTLTENAT
                     NGFVTKADLGLVITRAGSGTPVWSGTFGDLTAAGPLKLGTFAPGEARDYTFSTTLVQS
                     ASNAEQGKTASATYTWNSTQTAASTTNQ"
     gene            2472..3089
                     /locus_tag="VN041_05735"
     CDS             2472..3089
                     /locus_tag="VN041_05735"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWU28580.1"
                     /translation="MSATQRTRRFANSLITIVAIIAMGIVALMLVPGLLGLDRYVITG
                     GSMSGTFERGTLVFERQVPVSDLKVGDVITYLPPSDSGVTELVTHRIESITEDASAAN
                     GRVFHTKGDANASADPWTFTLASHTQPRVVGWVPTIGWAFIALSMPIVRILVIGVPAA
                     LIALLFLRDLMRGLRGSRPGRSITASEPVTTTASTTVVLETTATS"
     gene            3246..5087
                     /locus_tag="VN041_05740"
     CDS             3246..5087
                     /locus_tag="VN041_05740"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU28581.1"
                     /translation="MISTALRNTALAVLACILIAASALNSAQFSSAMYTAQSANGSST
                     ASAAADWTPPTVAVVDPGAPLKGAVTITANAADGETGVASVVLQKQVTGSGTWVTLCT
                     TTAAPYSCAWNTVADADGSYDLRAIATDRAGYSTTSASVRVLVSNAFGIIMTDPGDVL
                     RGSASLVATLQNANLLGLYNIRFEYSAGGTGTWTQIAGCLSILGSANCTQTWSTTGVA
                     SGTYDLRAVASPVLNPSAVVYSAVFTDVIVDNTRPTVTMTDPGSPLSGTVTLAASASD
                     ADSGVATVVIQYAPNGSSTWTTACTLTGAPYSCRFSTTALARGTYSFRAIATDVAGNT
                     TTSASVTNRQVDNTISSVSMADPGAYLEGTETLTVDANSTAGVTSVTTQYAPTGASTW
                     TTICATSQSPYTCTWDTTSLPDGLYDLRAVLVDGTGKVTISTLVTGRRIDNTPVRGAD
                     VQTANTTGIVGRLDTGDTITYTYSKQMVLGSILPGWSGAATPVTLRLRDGGLLGTGAA
                     GDTIDMLTGTTPVALGTVNLRGDYVKSGKTSTSNGTMVATRATVNGSPVTVVTVTVGT
                     LISGGALRSVSTASTMIWSPSGTATDLSGAACSTAPVSETGAADREF"
     gene            5138..>5454
                     /locus_tag="VN041_05745"
     CDS             5138..>5454
                     /locus_tag="VN041_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU28582.1"
                     /translation="MGRHILVVEDDDGIAVPLMRTLDREGYVVERVAEGAAALERAGD
                     DVDLVVLDLGLPDMDGLDVCRRLREQDFEGGIVILTARDGELDRVVGLDVGADDYIAK
                     PFAL"
BASE COUNT          891 a         2039 c         1757 g          767 t
ORIGIN      
        1 gtccagccga ggcagcgcca gtccgatcgt gggcgcgacg atcagccccg cgagcgcggt
       61 gacggtgacc agctgccccg cctgcccggg cgtcacgccc agctcggcgg cgatctcggt
      121 gagcacgcca ttgggcagga actcggcgct cacgagcagg aagctcatgg ccatcatcgt
      181 cagcagcgcg gcgtacggca tgcgccgcac cgtctcggtg acggttggca ggggtgcggt
      241 cgtggtcatg tttccagggt tcccgaagag cggatgccgt acccgaccac gcgtccggag
      301 catccgaccg ttcgtccggg acgtccagga gcgcgcggag cgctccgtgc tcgggccact
      361 cagcgcagga gcgtggagat catcaccacg gcgccgacga gcagcgcccc gaggacgccg
      421 tagatcgcga tcgtcagacc gcggtagccg ccggacgtcg cccccgaggg tggcggcacc
      481 acggccgggt ccggatccgg cgcggtcagc cggggatcgg gtgcgggggc cgacggagag
      541 ccgagagaat cggatgctgc gcgtcgggtc gcccgcgtga ccgatggcgc gccctcggga
      601 acccgtgttc cggatccggc cgggtagtcg cggtcggaac gccacgccgg gcgcgccacc
      661 ggcgggtcgt ccggcggagg cggcgggatc accggctcgg tcaggaacga ctccggcagc
      721 acggggtcag ggatcaccgg ctcccgctga tccagctccg ggacatcggc atccgggatc
      781 tcgagatcgg ggacgaacgt ctcggcatcc gggatctcca gatcgggaac gtcggcgtcc
      841 gccaccgcgg cgccagccgc cccagcgccc ggagactgaa gctccggcac atccgcatcc
      901 gtcgcaacag catccggaac cggcgtgccc gtcgcctcag ttcccggctc cgtcagccca
      961 ccggcaccgg aaccgtcccc cgacggcggt gcggcgccct cggcgccgcc caccgtcgtg
     1021 gcgtccggct cctgcgcgcc ctcgtcggga tctgccatgt cagcctccga tcgctcccgc
     1081 atcggtcgtg cccacgtcgg tgcggtggaa gttgaggaac gaacgggatg ccgtgggtcc
     1141 ccgctgcccc tggtaacggt tgccgtacgg tccggagccg tacgcgttct cggccggtga
     1201 ggtgagccgg aagaagcaga actggccgat cttcatgccg ggccacagct tgatcggcag
     1261 ggtcgccacg ttcgcgagct ccagggtcac gtgcccggtg aagccggggt cgacgaaacc
     1321 ggccgtcgaa tgcgtgatca ggcccaggcg cccgagcgac gacttgccct ccagccgcgc
     1381 agcgacgtcg tcgggcagcg acacctgctc gaaggtcgca cccagggcga actcccccgg
     1441 gtgcaggatg aacggctcgt ccggggcgac ctcgatcagg cgggtcagct ccggctgctc
     1501 caccgaggga tcgatgaacg ggtacttgtg gttgtcgaac agccggaaat agcggtccag
     1561 acgaacgtcc acgctcgagg gctgcaccat ctcctcctcg tagggggaca gcccgatgcg
     1621 gcccgatgcg agttctgcct tgatgtcgcg gtcgctgaga agcacgtctt cagcgtaacg
     1681 gtgctgttgc cggaccgcag agctgtgtcc gaacgctggc atccgtgtta cccagccgtg
     1741 gatcaggcgt tgcgcgcgtc tccgtagctt gattcctacg agcttccccc caccgctcgg
     1801 ccaaccacct caacgagaag gaaaccgatg aacgacagca tcaccgctca gaccgagacc
     1861 ccgatccgct cgcgcaagcg cctgatcgtg ccgctcgccg gcctgctcat cgccgccgcg
     1921 atcaccgtcg gatccggcgc cgacttcgtc gccaactccg tcaacgcggc caacgccttc
     1981 agcaccggca ccctgacgca gagcaactcc aagtcgggca gcgcgatctt caacgccgcc
     2041 aacctcaagc cgggcgacac gctcaacggc accgtgacca tcaccaactc gggcagcctg
     2101 ccggccgact tcaccctcac cgagaacgcc accaacggct tcgtcaccaa ggccgacctg
     2161 ggcctcgtga tcacccgcgc cggctccgga acccccgtgt ggtccggcac cttcggcgac
     2221 ctgaccgccg ccggcccgct gaagctcggt accttcgctc ccggcgaggc ccgcgactac
     2281 accttcagca ccacgctcgt gcagtcggcg agcaacgccg agcagggcaa gacggcctcg
     2341 gcgacctaca cctggaactc gacgcagacc gctgcgagca cgaccaacca gtgaccagtg
     2401 atcggggcgc aaccccccgc gccccgatca ctctgaaacc ggtcacccga agaccggaag
     2461 agaggacgag catgagcgcc acgcagagga cacgacgatt cgcgaactcg ctgatcacga
     2521 tcgtcgcgat catcgcgatg gggatcgtgg cgctcatgct cgtccccggc ctgctcggcc
     2581 tggaccgcta cgtcatcacg ggcggatcca tgtcgggcac cttcgagcgc ggcaccctgg
     2641 tcttcgaacg ccaggtgccc gtctcagacc tgaaggtcgg cgacgtcatc acgtacctgc
     2701 cgccgtccga ctccggcgtc accgagctgg tgacccatcg catcgagtcg atcaccgagg
     2761 acgcctcggc cgcgaacggc cgcgtcttcc acaccaaggg cgacgcgaat gccagcgccg
     2821 acccgtggac cttcaccctc gcatcccaca cgcagccccg cgtcgtggga tgggtaccca
     2881 ccatcggctg ggccttcatc gccctgtcga tgccgatcgt gcggatactg gtcatcggcg
     2941 ttcccgcggc actcatcgcc ctgctgttcc tgcgtgacct catgaggggg ctgcgaggga
     3001 gccgcccggg tcggagcatc accgcgtcgg agcccgtcac caccacggca tccaccaccg
     3061 tggtcctcga gaccaccgcc acctcgtgac agcccgctgc tgacgcagcc gccctgtccc
     3121 gaacacggca gcacagtccc gacgcgatcg cgccgggact gtatccgcgt tgaggcacgc
     3181 ctgtagtcgg gctgtaaccg gctgcctcgt agcgtcgtga gtaagacatg gaaggagtag
     3241 cagcgatgat cagcaccgca ctgcggaaca cggccctggc cgtgctggcg tgcatcctca
     3301 tcgccgcctc cgcgctgaac agcgcgcagt tctcctcggc catgtacacg gcgcagtccg
     3361 cgaacggctc gagcacggca tccgccgccg ccgactggac gccgccgacc gtcgcggtcg
     3421 tcgaccccgg cgctccgctg aagggcgccg tgacgatcac cgcgaacgcg gccgacggcg
     3481 agaccggcgt cgcgagcgtc gtgctgcaga agcaggtcac gggctccggc acctgggtca
     3541 cgctctgcac caccaccgcc gctccctaca gctgcgcctg gaacacggtc gccgacgccg
     3601 acggctccta cgacctgcgc gcgatcgcga ccgaccgcgc gggctactcg acgacctccg
     3661 cctcggtgcg cgtgctcgtc agcaacgcct tcggcatcat catgaccgac ccgggcgacg
     3721 tgctgcgcgg cagcgcctcc ctggtggcca ccctgcagaa cgcgaacctt ctcgggctgt
     3781 acaacatccg cttcgagtac tccgccggcg gcacgggcac ctggacgcag atcgccggat
     3841 gcctcagcat cctgggatcg gcgaactgca cccagacctg gtcgacgacc ggcgtggcca
     3901 gcggtaccta cgacctccgc gctgtcgcga gccctgtgct gaacccgtcg gcggtggtct
     3961 actcggcggt gttcaccgac gtgatcgtcg acaacacccg gccgaccgtc acgatgaccg
     4021 acccgggctc gcccctcagc ggcacggtca ccctcgcggc atccgcttcc gacgccgact
     4081 ccggcgtcgc cacggtcgtc atccagtacg caccgaacgg cagctccacc tggaccaccg
     4141 cgtgcacgct gaccggcgct ccgtactcct gccggttcag caccaccgcc ctcgcccgtg
     4201 gcacctactc gttccgcgcg atcgcgaccg acgtcgccgg caacacgacc acctccgcca
     4261 gcgtcacgaa ccgccaggtc gacaacacga tctcctcggt gtcgatggcc gacccgggcg
     4321 catacctcga gggcaccgag acgctcaccg tcgatgccaa ctccaccgcc ggcgtcacct
     4381 cggtcaccac ccagtacgcc cccacgggcg cctcgacgtg gacgacgatc tgcgccacgt
     4441 cccagtcgcc gtatacctgc acctgggaca ccacctcgct gcccgacggc ctgtacgacc
     4501 tgcgtgcagt gctcgtcgac ggcacgggca aggtcacgat ctccacgctg gtcaccggtc
     4561 ggcgcatcga caacaccccc gtgcgcggcg ccgacgtgca gaccgcgaac accaccggca
     4621 tcgtcggacg gctggacacc ggcgacacga tcacctacac gtacagcaag cagatggtcc
     4681 tcggcagcat cctgcccggc tggagcggcg cggcgacgcc ggtgacgctg cggctgcgcg
     4741 acggcggact gctggggacc ggcgcggccg gcgacacgat cgacatgctg acgggcacca
     4801 cccccgtcgc gctcggcacg gtcaacctgc ggggcgacta cgtgaagtcg ggcaagacgt
     4861 cgacctccaa cggcaccatg gtcgccacca gggccaccgt gaacggctcg cccgtcaccg
     4921 tggtgaccgt caccgtcgga acgctgatca gcggcggcgc cctgcgctcc gtgtcgacgg
     4981 cgtccaccat gatctggtcg ccctcgggca cggccaccga cctctcgggc gcagcatgct
     5041 cgacggcccc ggtctcggag accggcgccg cggacaggga gttctgatga tgcacaccgg
     5101 catatcggat gacgatgacc ttgatagcct cgaccacgtg ggacgacaca tcctggtcgt
     5161 ggaggacgac gacggcatcg cggtgccgct gatgcgcacg ctcgatcgcg agggatacgt
     5221 cgtcgaacgg gttgccgagg gcgcggccgc gctcgagcgc gcgggtgacg acgtcgacct
     5281 ggtcgtgctc gacctcgggc tgcccgacat ggacggtctt gacgtgtgcc ggcggctgcg
     5341 cgagcaggac ttcgagggcg gcatcgtcat cctcacggcc cgcgacggcg agctggaccg
     5401 cgtcgtcggc ctggatgtcg gcgccgacga ctacatcgcg aagccgttcg cgct
//
LOCUS       DATMSX010000187         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_107206,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000187 DATMSX010000000
VERSION     DATMSX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107206"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VN041_05750"
     CDS             complement(<1..864)
                     /locus_tag="VN041_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28583.1"
                     /translation="MRSSAAHPGMRIGLIGLAVGAAWFAVSLFTGAQGASAATGDSDA
                     GLLGSLVSQVESTATEAVQAVTAPVKPVVAPVEKVVTPVAQVAAPVVETVKAVPKAAA
                     SPASVTKVVAHVPEAVAQTVRSTVAVVESAPKTVADVVDSVPAVLDAVEVGDITAPVT
                     RLIDTVVNDTVGQVPLAGDLVTGLLGDAPTGDLLTPVIGGVDDVVDAVHGTPGALIPA
                     PPGAPAEDAVSEAVPVPGAASAASAAIAASASALVALPATDADAAPTTVVADDSPADS
                     PTGGELPPVAPA"
     gene            complement(1029..1481)
                     /gene="rplI"
                     /locus_tag="VN041_05755"
     CDS             complement(1029..1481)
                     /gene="rplI"
                     /locus_tag="VN041_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWU28584.1"
                     /translation="MAKLILTNEVAGLGSAGDVIEVKNGYARNYLIPQGFAVAWTRGG
                     AKQVESIQAARKARQIHDLDEATALKNKLEGTKVRLAVKAGAEGRLFGSVKTADVADA
                     VAAADLGSIDKRKVHFAAPIKSVGEHEATVRLHDDVTAVITLQVVAAK"
     gene            complement(1493..1747)
                     /gene="rpsR"
                     /locus_tag="VN041_05760"
     CDS             complement(1493..1747)
                     /gene="rpsR"
                     /locus_tag="VN041_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWU28585.1"
                     /translation="MAGKSSGDRRKPRKGGKPTAPAKSIRVGVIDYKDVATLRKFISE
                     RGKIRARRITGVSVQEQRLIAKAIKNAREMALLPYAGAGR"
     gene            complement(1809..2315)
                     /locus_tag="VN041_05765"
     CDS             complement(1809..2315)
                     /locus_tag="VN041_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWU28586.1"
                     /translation="MAGETVITVVGNLTADPELRYTQNGLPVANFTIASTPRTFDRQA
                     NEWKDGEALFLRASVWREFAEHVAGSLTKGMRVIAQGRLRQRSYQDRDGNNRTAIELE
                     VDEIGPSLRYATAQVTRAASNGGGGGSLQGGGRPQQQVSEEPWSTPGSSSADAWSTPG
                     SFGDDTPF"
     gene            complement(2321..2689)
                     /gene="rpsF"
                     /locus_tag="VN041_05770"
     CDS             complement(2321..2689)
                     /gene="rpsF"
                     /locus_tag="VN041_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWU28587.1"
                     /translation="MTHQYELMVILTPELDERQVAPNLDKFLKVITNDGGSIDKVDVW
                     GKRRFAYEINKKNEGIYAVVNFTATSEATQELDRQLKLSELVFRTKVLRAEEAQAMVA
                     AEAKRAADKAARKPKTAAKA"
     gene            complement(3164..>3901)
                     /locus_tag="VN041_05775"
     CDS             complement(3164..>3901)
                     /locus_tag="VN041_05775"
                     /inference="COORDINATES: protein motif:HMM:NF042780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HWU28588.1"
                     /translation="DDRRRIAEILRLAAVADPARRVVVKLRSRTGEVEAHQERDGYTE
                     LLENAPDNLVFSFAPMAEALATAEGLVTVSSTAAIEAMAAGVPVIALDEFGVRPELLN
                     GVFADSGLLGGADDVVARRFRHPNPRWATTNYFHDPRDSRWWEQTQRLVAQRRRGTLP
                     ARTVPRARGGALHEAWHRRNVLGAEDRSISGRMAGVIVTPVIALLASVKRRRGAVVPD
                     AMAADFTLAQSPVVEPVGRLVGTRPRL"
BASE COUNT          592 a         1343 c         1328 g          638 t
ORIGIN      
        1 ggcaggcgcg acaggcggca gctcgccacc cgtcggcgaa tcagcagggc tgtcgtccgc
       61 gacgacggtg gtgggcgcgg cgtcggcatc cgtcgcagga agagcgacca gcgcggatgc
      121 ggacgcagcg atggcggcag acgcagcgga tgccgcgcca gggacgggaa cggcctcaga
      181 cacggcgtcc tccgccggag cccccggcgg tgcgggaatg agcgccccgg gggtgccgtg
      241 cacggcatcg acgacgtcgt cgaccccgcc gatgaccggc gtgagcaggt ctcccgtcgg
      301 cgcatcgccg agcagtcccg tgaccaggtc gccggcgagc ggcacctgtc cgacggtgtc
      361 gttcaccacg gtgtcgatga gccgcgtgac gggtgccgtg atgtcgccga cctcgaccgc
      421 atcgagaacg gcggggacgg agtcgaccac gtccgccacg gtcttcgggg cgctctcgac
      481 gaccgcgacc gtgctccgca cggtctgtgc gacggcttcg ggcacgtgcg cgacgacctt
      541 cgtgacggat gccggagaag cggcggcctt cggtacggcc ttgacggtct cgaccacggg
      601 tgccgcgacc tgcgcgaccg gtgtgacgac cttctcgacc ggcgcgacca ccggcttcac
      661 gggggcggtg accgcctgaa cggcctcggt cgcggtgctc tcgacctggc tgaccaggga
      721 gcccagcagt ccggcatccg agtcgccggt cgcggcggat gcgccctgcg caccggtgaa
      781 gaggctgacc gcgaaccagg cggcgccgac cgcgagaccg atgaggccga tgcgcatgcc
      841 ggggtgggcg gcgctgctac gcattgcgga gtcgcctgtc tgcatcggaa tcaccccccc
      901 ggtcttcact cggaggctac acccgcacgc gcgaacatgt gaagacccaa tattctggct
      961 gtgcaccgcc cgggaatgca gaacgtcccg ccctccgaag aggacgggac gttcgacgcg
     1021 tgcggcgatt acttcgcagc gaccacctgg agggtgatca cggcggtgac gtcgtcgtgc
     1081 agacggacgg tcgcctcgtg ctcgccgacc gacttgatcg gggcggcgaa gtgcaccttg
     1141 cgcttgtcga tcgagccgag gtcggcagcg gcgacagcgt cggccacgtc ggccgtcttc
     1201 accgagccga acaggcgacc ctcggcgccg gccttgacgg ccagacgcac cttggtgccc
     1261 tcgagcttgt tcttcagagc cgtcgcctcg tcgaggtcgt ggatctggcg agccttgcgg
     1321 gcagcctgga tcgactcgac ctgcttggcg ccaccgcggg tccacgcgac agcgaagccc
     1381 tgcgggatga ggtagttgcg ggcgtacccg ttcttgacct cgatgacgtc accggcgctt
     1441 ccgagcccgg cgacctcgtt cgtgagaatc agctttgcca ttttcgtacc ccttaacggc
     1501 cagcgccggc gtagggcagc agggccatct cgcgagcgtt cttgatcgcc ttggcgatca
     1561 gacgctgctc ctggaccgag acaccggtga tgcgacgggc gcggatcttc ccacgctccg
     1621 agatgaactt gcgaagcgtt gcgacgtcct tgtagtcaat gacgcccacg cggatcgact
     1681 tcgcgggagc ggtgggcttg ccacccttcc gcggcttgcg gcggtcgccg ctcgactttc
     1741 cagccatgag ttttccttaa agatgagatc ggatgcctga gccctgaacg ggctcgggga
     1801 tccagaagtt agaacggggt gtcgtcgccg aagctgcccg gggtgctcca cgcgtcagcg
     1861 ctcgacgaac cgggggtcga ccacggctcc tccgagacct gctgctgcgg gcggccaccg
     1921 ccctggaggc ttccgccccc accaccgttg gatgcggccc gcgtgacctg tgccgtcgca
     1981 tatcgcagcg acggaccgat ctcgtcaacc tccagctcga tcgccgtgcg gttgttgcca
     2041 tcgcggtcct ggtaggagcg ctgacgcaga cggccctgcg cgatcacgcg catgcccttg
     2101 gtcagcgaac ctgccacgtg ctcggcgaac tcacgccaca ccgatgcgcg gaggaacagc
     2161 gcttcgccgt ccttccactc gttggcctga cggtcgaacg tgcgcggggt cgatgcgatg
     2221 gtgaagttcg ccacaggcag gccgttctgc gtgtaacgca gctcggggtc cgccgtgagg
     2281 ttgcccacca cggtgatgac ggtttcgccg gccatgatgc ttacgccttc gcggccgtct
     2341 tgggcttgcg agcggccttg tcggcagcgc gcttcgcctc ggcggcgacc atggcctgtg
     2401 cctcttcggc gcgcaggacc ttggtgcgga agacgagctc gctcagcttc agctgacggt
     2461 cgagctcctg ggtggcctcg ctcgtcgcgg tgaagttgac gacggcgtag atgccctcgt
     2521 tcttcttgtt gatctcatag gcgaaacggc gcttgcccca cacgtcgacc ttgtcaatcg
     2581 agccaccatc gttggtgatg accttcagga acttgtcgag gttgggggca acctggcgct
     2641 cgtccagctc gggggtcaga atgaccatga gctcgtactg gtgcgtcact tacccacctc
     2701 cttcggacta gaacggctcc cgggactttc ccggaagcag gagggtgtgt tgcgtgtcat
     2761 ccgctccttc ggcatggcgc cgccgggcag acaacctcag cagtctaccg gatcggagcg
     2821 gatgccgcag gccggcccct cgcccagccc ggggcgacga aagtctcggc gaggatcgac
     2881 attcgtcgag agttccgtcc ggccgttgat attccagtat tccggtcgcg aatatgagag
     2941 cgcgcccccc ggcgcaccac agaactgggc tgccgcccac cgaacgaagg aatgaacatg
     3001 gacgctgttc tcggtctcat caccggtctg ctcaacactc tcctcggtct cctcggcggc
     3061 ctcggcggct gaccatcgcg ggccggggag ccggccacag accagagatc aagcgaagcc
     3121 ccgtgagaac acggggcttc gccttgtccg gctccgaccg cggttacagt cgggggcggg
     3181 tgcccaccag tctcccgacc ggctcgacga cgggactctg agcgagtgtg aagtcagcag
     3241 ccatggcatc cggcaccaca gctccccgtc gacgcttcac gctcgccagg agagcgatca
     3301 ccggggtgac gatgaccccg gccatccgcc ccgagatcga gcggtcctcg gctccgagca
     3361 cattgcgccg atgccatgcc tcgtgcagcg cgccaccgcg cgcccgcggc acggtgcggg
     3421 cgggcagcgt gcctctgcgg cgctgcgcga ccaggcgctg cgtctgctcc caccagcgcg
     3481 agtcgcgcgg gtcgtggaaa tagttggtcg tcgcccagcg cgggttggga tgccggaacc
     3541 tgcgtgcgac cacgtcatcc gcaccgccca gcaggccgct gtccgcgaag accccgttga
     3601 gcagttccgg gcgcactccg aactcgtcga gtgcgatcac cggcacgccg gccgccatcg
     3661 cctcgatcgc cgccgtcgag ctgacggtga cgagcccctc ggccgtggcg agcgcctccg
     3721 ccatcggggc gaacgagaac accaggttgt cgggcgcgtt ctcgagcagc tcggtgtagc
     3781 cgtcgcgctc ctggtgcgcc tcgacctcac ccgtccgcga acgcagcttc accacgacgc
     3841 ggcgtgcggg atcggcgacg gcagcgaggc gcaggatctc cgcgatccgg cggcggtcgt
     3901 c
//
LOCUS       DATMSX010000188         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_21394,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000188 DATMSX010000000
VERSION     DATMSX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21394"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VN041_05780"
     CDS             <1..172
                     /locus_tag="VN041_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWU28589.1"
                     /translation="ARGGVLRALRHAEGHRMPSDAVLADWPDAVQRDRAIDSLIGDGL
                     VEAVDGLLRLPV"
     gene            complement(246..668)
                     /gene="rbfA"
                     /locus_tag="VN041_05785"
     CDS             complement(246..668)
                     /gene="rbfA"
                     /locus_tag="VN041_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWU28590.1"
                     /translation="MAGERQARLADRIRVILAERLEKGLRDPRLGFVTITDVRVSGDL
                     QHASVFYTVLGSDEERVASGAALNSATGMLRKEVGRQLNVRLVPTLEFIPDALPESAG
                     HIADLLRQARERDEEVAKLAAGATHAGDADPYVHDDED"
     gene            complement(802..>2850)
                     /gene="infB"
                     /locus_tag="VN041_05790"
     CDS             complement(802..>2850)
                     /gene="infB"
                     /locus_tag="VN041_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWU28591.1"
                     /translation="GGFQRPGGAPGAPAGGFAGRPGGGGGRGRGPGGGTAGAFGKGGG
                     KSKQRKSRRAKRQEFEMRSAPVVGGVNVSKGNGEIIRLRRGASIADFADKLEALNGYT
                     VQPGTLVTILFNLGEMATATESLDEATFEVLGAELGYKIQMVSPEDEDKELLEGFGLD
                     LEAELEAESEDDLEIRPPVVTVMGHVDHGKTRLLDAIRKSNVIDGEAGGITQHIGAYQ
                     IWTEHDGIERAVTFIDTPGHEAFTAMRARGAQVTDIAILVVAADDGIMPQTVEALNHA
                     QAAGVPIVVAVNKVDKPEANPAKVRQQLTEYGLVAEEYGGDVMFVDVSARQGTGIQEL
                     IDAVLLTADAGLDLTANPNKAARGVAIEAKLDKGRGSVATVLIQSGTLRVGDAIVAGT
                     AYGRVRAMLDENGEQVTEAAPSRPVQVQGLNSVPRAGDVFIVTDEDRMARQIAEKREA
                     VERNAQLAKARKRISLEDFTRALEEGKVETLNLIIKGDVSGAVEALEESLLKIEVDDS
                     VQLRIIHRGVGAITESDVNLATIDNAIIVGFNVRPDAKAREASNREGVDIRFYSVIYA
                     AIEEIENSLKGMLKPEFEEVQSGVAEIREVFRSSKFGNIAGVIVRSGTITRNAKARVI
                     RDGVVIADGLAIESLRRFKDDVTEVRTDFEAGIGLGKYNDIQIGDEIETTEMVEKPRS
                     "
BASE COUNT          412 a          982 c          958 g          498 t
ORIGIN      
        1 ggcgcgcggg ggcgtgctgc gcgcgctgcg ccatgccgag ggtcatcgga tgccgtcgga
       61 tgccgtgctg gccgactggc cggacgctgt ccagcgcgac cgggcgatcg actccctgat
      121 cggcgacggg ctggtcgagg ccgtggacgg gttgctgcgt ctgcccgtgt gaggcagtcg
      181 ccgagagagg cgtctcagag ctgtcgagtg ccccggcggg cactcagcgc tcgtcaggcc
      241 ccgtctcagt cctcgtcgtc gtgcacgtac ggatcggcgt cgcctgcgtg cgtggcgccc
      301 gcggcgagct tcgccacctc ttcgtcccgc tcgcgggcct gacgcagcag gtcggcgatg
      361 tgcccggccg actccggcag agcgtccggg atgaactcca gcgtcggcac gaggcgcacg
      421 ttcagctgac ggccgacctc cttgcggagc atccccgtcg ccgagttcag cgcggcgccg
      481 ctggcgacgc gctcctcatc gctgccgagc accgtgtaga acacggatgc gtgctgcagg
      541 tcgcccgaca cacgcacatc ggtgatggtc acgaaaccca gtcgcgggtc gcgcagcccc
      601 ttctcgaggc gctcggcgag gatgacgcgg atgcgatccg cgagccgtgc ctgtcgttct
      661 ccagccatgt tcctattcct ataccttccg agcggcttcc gtgggaaccg cgaaggcggc
      721 tgccttccct tcacgagcat tggggtaggg ccccactctg cgagtgaagg gaaggcagcc
      781 gccggagcag ctttccagga ctcagctgcg aggcttctcg accatttcgg tggtctcgat
      841 ctcgtcgccg atctggatgt cgttgtactt gcccaggccg ataccggcct cgaagtcggt
      901 gcggacctcg gtgacgtcat ccttgaagcg acgcagcgac tcgatggcca ggccatcggc
      961 gatgacgacg ccgtcgcgga tgacgcgtgc cttggcgttg cgcgtgatcg ttccggagcg
     1021 gacgatgaca ccggcgatgt tgccgaactt cgaggagcgg aacacctcgc ggatctcggc
     1081 aacacccgac tggacctctt cgaactccgg cttgagcatg cccttgaggg agttctcgat
     1141 ctcctcgatg gccgcgtaga tcaccgagta gaaccggatg tccacgccct cgcggttcga
     1201 ggcctcgcgc gccttggcat ccggacggac gttgaagccc acgatgatcg cgttgtcgat
     1261 cgtcgccaga ttcacatccg actccgtgat ggcgccgacg ccgcggtgga tgatgcgcag
     1321 ctgcaccgag tcgtcgacct cgatcttgag cagcgactcc tccagcgcct cgacggcacc
     1381 ggacacgtcg cccttgatga tgaggttgag cgtctcgacc ttgccctctt cgagagcacg
     1441 ggtgaagtcc tcgagcgaga tgcgcttgcg ggccttggcc agctgggcgt tgcgctcgac
     1501 ggcctcgcgc ttctccgcga tctgacgggc catgcggtcc tcgtcggtga cgatgaacac
     1561 gtcaccggcg cggggcacgg agttcagacc ctgcacctgc acggggcggg acggcgcggc
     1621 ctcggtgacc tgctcgccgt tctcgtcgag catggcgcgg acgcgaccgt acgcggtacc
     1681 ggcgacgatg gcgtctccga cgcgcagcgt tccggactgg atcagcacgg tggccaccga
     1741 accgcggccc ttgtcgagct tggcctcgat cgcgacacca cgggccgcct tgttcgggtt
     1801 ggccgtgagg tccagccccg cgtcggcagt cagcagcacg gcatcgatca gttcctggat
     1861 gccggtgccc tggcgggccg acacgtccac gaacatgacg tccccgccgt actcctcggc
     1921 gaccagaccg tactcggtga gctgctggcg caccttggca gggttggcct cgggcttgtc
     1981 gaccttgttg accgcgacca cgatcggcac cccggccgcc tgggcgtggt tcagcgcctc
     2041 caccgtctgc ggcatgatgc cgtcgtcggc ggcgaccacg aggatcgcga tgtcggtcac
     2101 ctgcgcacca cgggcacgca tggcggtgaa cgcctcgtga cccggggtgt cgatgaaggt
     2161 gacagcgcgc tcgatgccgt cgtgctcggt ccagatctgg tacgcaccga tgtgctgggt
     2221 gatgccgccg gcctcgccat cgatgacgtt gctcttgcgg atcgcgtcga gcagtcgggt
     2281 cttaccgtgg tcgacgtgac ccatgacggt gaccaccggc gggcggatct cgaggtcgtc
     2341 ctcgctctcc gcctccagct cggcctcgag gtcgagaccg aagccctcca ggagctcctt
     2401 gtcctcgtcc tcgggcgaga ccatctggat cttgtagccg agctcggcac ccaggacctc
     2461 gaaggtcgcc tcatccaggg actcggtggc cgtggccatc tcgcccaggt tgaagaggat
     2521 cgtgacgagc gttccgggct ggacggtgta gccgttcaga gcctcgagct tgtcggcgaa
     2581 gtcggcgatg gaggcaccgc ggcgcagacg gatgatctcg ccgttgccct tcgagacatt
     2641 gacgccgccg acgaccggcg ccgaccgcat ctcgaattcc tgccgcttcg cccgacgcga
     2701 cttgcgctgc ttgctcttgc cgccgccctt gccgaaggca cctgccgtgc caccgccggg
     2761 tccacgacca cgaccgccgc caccaccggg acgaccggcg aaaccgccgg ccggagctcc
     2821 gggagcaccg ccaggacgct ggaagccgcc
//
LOCUS       DATMSX010000189         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_21399,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000189 DATMSX010000000
VERSION     DATMSX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21399"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /gene="valS"
                     /locus_tag="VN041_05795"
     CDS             complement(<1..2409)
                     /gene="valS"
                     /locus_tag="VN041_05795"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWU28592.1"
                     /translation="MSVIPDKPALEGLEAKWGERWSEQGTFLFDRLRAAEAGRTGVYS
                     IDTPPPTASGSLHIGHVFSYTHTDIKARFERMRGKNVFYPMGWDDNGLPTERRVQNYY
                     GVRCDPSLPYDPDFTPPFEGGDNKSSRAADQLPISRRNFIELCEKLTIEDEKQFEALF
                     RQLGLSVDWTQTYRTISDDTIRQSQLAFLRGLERGEAYQSLAPTLWDIDFRSAIAQAE
                     LEDREQQAAYHRVAFHKSDGSGDIQIETTRPELLAACVALVAHPDDERYQPHFGSTVR
                     TPIFDVEVPVLAHHLAQPDKGSGIAMICTFGDVTDIIWWRELDLPNRTILGKDGRVLA
                     DVPEAIVSDAGRAAYAELAGKTVFSARKSMVELLQASGDLIEVGKPFNHAVKFFEKGD
                     RPLEIVSTRQWYIRNGARDEKLRDDLLENGRQMSWHPDFMRVRYENWVGGLTGDWLIS
                     RQRFFGVPIPLWYGLDENGERDYDRVITPSHDSLPIDPTSDVPAGYTAEQRGVPGGFD
                     AEQDIFDTWATSSLTPQLAGGWQRDEDLWNLVAPFDLRPQGQDIIRTWLFSTMLRSTL
                     EDQRAPWSNAAISGFIVDPDRKKMSKSKGNVVTPADILDKHGSDAVRYWSASSRLGMD
                     AAFDPQNPTQVKIGRRLAIKILNAAKFVLSFPVPEGAQVTHALDASMLATLDRVVREA
                     TAAYENYDQAKALEVTEAFFWAFCDDYLELVKERAYDQTDVGQASAALALRLALSTLL
                     RLLAPIVSFATEEAWSWFEEGSIHTASWPEALDIEGDPAVLATASEALVGIRRAKTEA
                     KASQK"
     gene            2575..4209
                     /locus_tag="VN041_05800"
     CDS             2575..4209
                     /locus_tag="VN041_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28593.1"
                     /translation="MPSFPVRRLLPLAALAAASALILSACAGPATSGGADAGTDPDAE
                     LSWAIEGANLSNGHMDPQTSQLDVSAMVQRQVLDSLVFQEADGSFSPWLAKSWKVSDD
                     GKAYTFELRDDVTFTDGTPFDAAAVKANFDRIIDPKTESASAAAMLGGELYAGTKVVS
                     DHTVEVDFTSPYAPFLQAASTAQLGFWSPKVLKNSAAQLKAGGPDVNIGTGPFTLTEY
                     TADQEIVYTRNDAYAWGPDGAKAPKVKTLRVEILPEASVRAGVLSSGEAQVASQLPPS
                     TVKELGKDVTVSAKEYPGLPYSLFLNEKHGVFADQNVRQAFELSIDVDAAVKQIFFGE
                     FPRAWSILGPTTPGYDKALEGTWKFDPEKAGQLLDQAGWTARDGEGYRTKDGKRLSAR
                     WIAWTPVSDEHTAMANAIQSDLKKVGFEVVRETLEPAAYNEQYGPKTYDLTDWDYSGV
                     DADLLRNHLGTDGFQNASQVSDPAVDKLLQDGLSTTDPAARAKIYQEVQQWNAEHVAI
                     VPLYVSSLITASDKGVKGLTYDLYGRPQFYTASLAG"
     gene            4223..5185
                     /locus_tag="VN041_05805"
     CDS             4223..5185
                     /locus_tag="VN041_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28594.1"
                     /translation="MSRALIRSAGILASVVIVLWGAATLAFLAFRIIPGDAVSVMLGP
                     QAQVSEEVKDGIRAELGLDRPPLVQYLSYLGGLLHGDLGRSYQLRMPVTEVIGRQLGP
                     TVQLTALALLIALVLALAAALFARRGFARGVVVAAELIVLSSPVFWIGLLLLAVFAFT
                     LGWFPVAGSRTPATLVLPAVTLALPVAALIGQVLRDGIDAAERQPFAETVRARGATPA
                     RLVLRHSLRHGAASALTLTAYLVGSLLGGAVLVETVFARPGLGRVTLAAIVNRDLPVV
                     AGIIMLSALVFVIVNVLVELLHPLIDPRLGASGRFEAPAIGERR"
BASE COUNT          764 a         1792 c         1853 g          864 t
ORIGIN      
        1 cttctgcgag gccttcgcct cggtcttggc gcggcggatg ccgaccaggg cctcgctcgc
       61 ggtggcgagc acggccgggt cgccctcgat gtccagcgcc tcgggccagg aggccgtgtg
      121 gatcgagccc tcctcgaacc agctccacgc ctcctcggtc gcgaacgaca cgatcggggc
      181 gagcaggcgc agcagcgtcg acagcgcgag ccgcagcgcg agggcggcgg atgcctggcc
      241 cacgtccgtc tggtcgtagg cgcgctcctt gacgagctcc aggtagtcgt cgcagaacgc
      301 ccagaagaac gcctcggtga cctcgagggc cttggcctgg tcgtagttct cgtacgccgc
      361 ggtggcctcg cgaaccaccc ggtcgagggt ggcgagcatc gatgcgtcca gggcatgcgt
      421 gacctgcgcg ccctccggca ccgggaacga gagcacgaac ttcgcggcgt tgaggatctt
      481 gatcgcgagg cgccggccga tcttgacctg cgtcgggttc tgcgggtcga acgcggcgtc
      541 catgccgagg cggctcgagg ccgaccagta gcgcacggca tccgagccgt gcttgtcgag
      601 gatgtcggcg ggagtcacca cgttgccctt ggacttcgac atcttcttgc gatcggggtc
      661 cacgatgaag ccggagatcg ccgcgttgct ccacggtgcg cgctggtcct cgagggtcga
      721 gcgcagcatg gtggagaaca gccaggtgcg gatgatgtcc tggccctgcg ggcgcaggtc
      781 gaacggggcg accaggttcc acaggtcctc gtcgcgctgc cagccgccgg ccagctgcgg
      841 ggtgagcgag gaggtcgccc aggtgtcgaa gatgtcctgc tcggcgtcga agccgccggg
      901 cactccgcgc tgctcggccg tgtagccggc gggcacgtcg ctggtcgggt cgatcggcag
      961 ggagtcgtgc gagggggtga tcacgcggtc gtagtcgcgc tccccgttct cgtcgaggcc
     1021 gtaccagagc gggatcggca cgccgaagaa gcgctgacgc gagatcagcc agtcaccggt
     1081 caggccgccg acccagttct cgtagcgcac gcgcatgaag tcggggtgcc aggacatctg
     1141 gcggccgttc tcgagcaggt cgtcacgcag cttctcgtcg cgggcgccgt tgcggatgta
     1201 ccactggcgg gtcgagacga tctcgagggg acggtcgccc ttctcgaaga acttcacggc
     1261 gtggttgaag ggcttcccca cctcgatgag gtcgccggac gcctgcagca gctccaccat
     1321 cgacttgcgg gcgctgaaca ccgtctttcc cgcgagctcc gcgtatgcgg cccggccggc
     1381 atccgacacg atcgcctcgg gaacgtcggc cagcacgcgg ccgtccttgc cgaggatggt
     1441 gcggttgggc agatcgagct cgcgccacca gatgatgtcg gtcacgtcac cgaaggtgca
     1501 gatcatggcg atgccggagc ccttgtcggg ctgcgcgagg tggtgcgcca gcacgggcac
     1561 ctcgacgtcg aagatcggcg tgcgcacggt ggagccgaag tgcggctggt agcgctcgtc
     1621 gtccgggtgc gcgaccaggg ccacgcaggc ggcgagcagc tcgggccggg tggtctcgat
     1681 ctggatgtca cccgatccgt cgctcttgtg gaacgcgacg cggtggtacg cggcctgctg
     1741 ctcgcggtcc tcgagctcgg cctgtgcgat cgcggagcgg aagtcgatgt cccagagcgt
     1801 cggggcgagg gactgatacg cctcgccgcg ctccagcccg cgcaggaacg ccagctggct
     1861 ctgccggatc gtgtcgtccg agatggtgcg gtaggtctgg gtccagtcga cgctgagacc
     1921 cagctggcgg aacagggcct cgaactgctt ctcgtcttcg atggtcagct tctcgcagag
     1981 ctcgatgaag ttgcggcggc tgatcggcag ctggtctgcg gcgcgggagg acttgttgtc
     2041 gccgccttcg aacggcggcg tgaagtcggg gtcgtagggg agcgaggggt cgcagcgcac
     2101 gccgtagtag ttctgcacgc ggcgctcggt gggcagaccg ttgtcgtccc agcccatcgg
     2161 gtagaagacg ttcttgccgc gcatccgctc gaagcgggcc ttgatgtcgg tgtgcgtgta
     2221 cgagaacacg tgaccgatgt gcaggctgcc ggatgccgtg ggagggggcg tgtcgatcga
     2281 gtagacgccg gtgcggccgg cttcggcggc acgcaggcgg tcgaacagga acgtcccctg
     2341 ctcggaccag cgctcacccc acttcgcttc gagaccctcg agtgcgggct tgtcgggaat
     2401 gacggacata ggggtctcct tgagcgatgt gtgcggcact gtgtgagcgt gcctgagtgt
     2461 gggttgtggg gcgagtctac cgggcatccg ccggtgcgac gtagatcgac ccggtccaag
     2521 aaaccgcgga taggatggaa cgtcaccctt tccgcactct cccgaggttc ccgaatgcct
     2581 tccttccccg tgcgccggct tcttccgctg gcggctctcg ccgcagcatc cgcactcatc
     2641 ctcagcgcct gcgccggacc cgccacctcc ggcggggcgg atgccgggac ggatcccgac
     2701 gccgagctca gctgggcgat cgagggcgcc aacctctcga acggtcacat ggacccgcag
     2761 accagccagc tcgacgtctc cgcgatggtg cagcgtcagg tgctcgactc cctggtgttc
     2821 caggaggccg acggctcgtt ctctccgtgg ctcgcgaaga gctggaaggt ctccgacgat
     2881 ggcaaggcgt acacgttcga gctgcgcgac gacgtgacgt tcaccgacgg caccccgttc
     2941 gacgccgcgg ccgtcaaggc gaacttcgac cgcatcatcg acccgaagac cgagtcggca
     3001 tccgcggcgg ccatgctcgg cggtgagctg tacgcgggca ccaaggtcgt ctccgaccac
     3061 acggtcgagg tggacttcac ctctccctac gcgccgttcc tgcaggcggc cagcaccgcc
     3121 cagctcggct tctggtcgcc caaggtcctc aagaactcgg ccgcgcagct caaggcgggc
     3181 ggtcccgacg tgaacatcgg caccggcccg ttcacgctca ccgagtacac ggccgaccag
     3241 gagatcgtct acacccgcaa cgacgcctac gcctggggcc ccgacggcgc caaggcgccg
     3301 aaggtcaaga cgctgcgcgt ggagatcctg cccgaggcct ccgtccgcgc cggggtgctc
     3361 agctccggag aggcgcaggt cgccagccag ctcccgccga gcacggtgaa ggaactcggc
     3421 aaggacgtca ccgtcagcgc caaggagtac ccgggcctgc cgtactcgct gttcctcaac
     3481 gagaagcacg gcgtcttcgc cgaccagaat gtgcgccagg cgttcgagct gtcgatcgat
     3541 gtggatgcgg ccgtgaagca gatcttcttc ggcgagttcc cgcgggcatg gagcatcctc
     3601 ggcccgacca ccccgggcta cgacaaggcg ctggagggca cctggaagtt cgaccccgag
     3661 aaggccggcc agctgctcga ccaggccggc tggaccgccc gcgacggcga gggctaccgc
     3721 acgaaggacg gcaagcgcct gtccgcgcgc tggatcgcct ggacgccggt ctcggacgag
     3781 cacaccgcca tggccaacgc catccagtcc gacctgaaga aggtcggctt cgaggtcgtg
     3841 cgcgagactc tggaacccgc tgcgtacaac gagcagtacg gcccgaagac ctacgacctc
     3901 accgactggg actactccgg cgtcgacgcc gacctgctgc gcaaccatct cggcacggac
     3961 gggttccaga acgcgtccca ggtcagcgac ccggccgtcg acaagctcct gcaggacggt
     4021 ctgtccacca ccgacccggc cgcgcgcgcc aagatctacc aggaggtgca gcagtggaac
     4081 gccgagcacg tggcgatcgt cccgctctac gtctcctcgc tcatcaccgc gtcggacaag
     4141 ggcgtcaagg gcctgaccta cgacctgtac gggcgtccgc agttctacac cgcgagcctg
     4201 gccggctgag tcgcgttccg acgtgtcgcg cgcactgatc cggagcgccg ggatcctggc
     4261 atccgtcgtc atcgtcctgt ggggcgcggc gacgctcgcc ttcctggcgt tccggatcat
     4321 ccccggcgac gcggtctcgg tgatgctcgg cccgcaggcc caggtgagcg aggaggtgaa
     4381 ggacggcatc cgcgccgagc tggggctgga tcgtccgccg ctggtgcagt acctgtcgta
     4441 cctcggcggg ctcctgcacg gcgacctcgg acggtcgtac cagctgcgga tgccggtgac
     4501 cgaggtcatc gggcgccagc tcggtcccac agtgcagctg accgccctgg cgctgctgat
     4561 cgcgctcgtt ctggctctcg ccgccgcgct gttcgcccga cgcgggttcg cgcggggcgt
     4621 ggtcgtggcg gccgagctga tcgtgctgtc atcgcccgtt ttctggatcg gcctgctgct
     4681 gctggcggtg ttcgcgttca cgctcggctg gttccccgtg gcgggttcgc gcacgcccgc
     4741 gacgctcgtc ctgccggccg tcaccctggc gcttcccgtc gccgctctca tcgggcaggt
     4801 cctgcgcgac ggaatcgatg cggccgagcg tcagccgttc gcggagaccg ttcgggcgcg
     4861 gggtgcgacc ccggcacggc tcgtgctgcg gcactccctc cggcatggcg ccgcgagcgc
     4921 cctgacgctg accgcgtacc tggtcggctc gctgctgggc ggggctgtgc tcgtcgagac
     4981 cgtgttcgca cgccccggtc tcgggcgcgt gacgctcgca gcgatcgtca accgcgatct
     5041 gcccgttgtc gccgggatca tcatgctcag cgctctcgtg ttcgtgatcg tgaacgtgct
     5101 ggtggagctg ctgcatccgc tcatcgaccc gcgcctgggc gcgtcgggac gcttcgaggc
     5161 gcccgcgatc ggggagcggc gatgaaggcg gcggggctct ggatcgccgg gctgtacctc
     5221 gcgctcgtcg cgctggccgc attcgcgccc gatctgctcg cgacacacga ccc
//
LOCUS       DATMSX010000190        12410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_69120,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000190 DATMSX010000000
VERSION     DATMSX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69120"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VN041_05810"
     CDS             complement(<1..855)
                     /locus_tag="VN041_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28595.1"
                     /translation="MDNVEEQHPEPRLGLVPGAHRLIRRLDGTESPLTGELVSDGEHR
                     RVLVDTAATDAALWEFAGAEHVAGVHDLVRTADGHGALLPWCVEPVETLIARRRAAGR
                     PLTSGEIVTLVGSLMRGIVEVADRAVTGRWWLEDGGRPVFVPGEGVSSTAASVAVVER
                     VRDECADRAMQRTLVRIAAGMSDHRMVLRQLDAWEGELTELAAPRALEREVPDMDGPW
                     PTPERVRALPFHRAGLAEVAGQLEERRSLRERLVLIGARLQERLRESMPSKPKVSSGV
                     DAGADGRTR"
     gene            981..1691
                     /locus_tag="VN041_05815"
     CDS             981..1691
                     /locus_tag="VN041_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HWU28596.1"
                     /translation="MAKRAPEAEKRPGFFSQIRSLFRFTREVYGWLPWAQLAILIGGV
                     LIGLILGFLVGGGAVLSLILWAVTGLMFGVLGAMFLMTRLSTKAMYAKIDGMPGASGH
                     VLSTSLGRSWQASDSPVGINPKTQEAVYRAIGRGGIVVVGEGTRGRLTRLVNEERQKA
                     QRVANGVPVTVLYVGHGEDEVPISDLAKRIKKIPKAIDKATMAAVIRRIDSVSQSLSS
                     LPIPKGIDPTKVRAQRPR"
     gene            complement(1755..2942)
                     /locus_tag="VN041_05820"
     CDS             complement(1755..2942)
                     /locus_tag="VN041_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HWU28597.1"
                     /translation="MKIAVLSRAPQSYSTQRLRAAAQQRGHTVKVLNTLRFAIDLTAD
                     APDLFFRGRQLSDYDAILPRIGNSITYFGTAVVRQFEQMDVYTPNTANGISSARDKLR
                     ANQILSRHNIAMPATAFVRNRADVRPAIERVGGAPVVIKLLEGTQGIGVILAPQVKVA
                     EAIIETLHSTKQNVLIQKFIAESRGRDIRALVVGDRVVAAMRRVAEGDEFRSNVHRGG
                     RVEPVTLDPVYEQTAVRSAQIMGLRVAGVDMLESDEGPLVMEVNSSPGLQGIEAATNL
                     DVAGAIIDYIANQVAFPDIDIRQRLSVSTGYGVAELMVHSGAEQVGMKLGDLGLWDRD
                     ITVLTLHRGVSVIPNPRKHVVLEAEDRLLCFGKLEEMRSMIPEKRRRRPRVRRLPKEP
                     LGE"
     gene            2968..3447
                     /locus_tag="VN041_05825"
     CDS             2968..3447
                     /locus_tag="VN041_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HWU28598.1"
                     /translation="MNRSSHSNTSTGWREWVSLPELGVDWIKAKIDTGARTSSLHAFD
                     VEEFTRDGEDWVRFAVHPWQETREDAVVHECPVHDRRAVRSSSGHAEHRIVVLLRMRL
                     VGHDVQGEVTLTNRDEMGFRMLIGRQVLRRGFVVDPAKSFLGGRAPREMRRRNRGRD"
     gene            complement(3490..3906)
                     /locus_tag="VN041_05830"
     CDS             complement(3490..3906)
                     /locus_tag="VN041_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWU28599.1"
                     /translation="MNDYPGERLGLPATGPRSIARPGRRIGALAIDYFASTLIALGFL
                     GYDPLALPAEAGWRQFAPMMVFALLQILFIPTAGGSPGHRILGMRVVRFGGGWVGLWR
                     PIVRTLLLVVVIPAVIWDADQRGLHDKIAGTVLIRS"
     gene            4024..5448
                     /gene="glnA"
                     /locus_tag="VN041_05835"
     CDS             4024..5448
                     /gene="glnA"
                     /locus_tag="VN041_05835"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWU28600.1"
                     /translation="MFKEPADVLRYIEENDVKFLDIRFTDLPGVQQHFNIPASTVDED
                     FFRDGQLFDGSSIRGFASIHESDMQLIPDVTTAYLDPFREAKTLVMVFDIYNPRTGEI
                     YSKDPRQVAKKAEKYLASTGIADTAYFAPEAEFYIFDDVRYSVTAGESFYRIDSEEAA
                     WNSGREEEGGNLANKTPFKGGYFPVAPVDKTADLRDDMTLRLIDAGFHLERSHHEVGT
                     AGQQEINYRFDTMVSAADDILKFKYIIKNTAQEWGKTVTFMPKPLYGDNGSGMHTHQS
                     LWLDGKPLFFDEAGYGQLSDIARWYIGGLLKHAHAVLAFTNPTLNSYHRLVKHFEAPV
                     NLAYSAGNRSAAIRIPLTGSNPKAKRIEFRVPDASGNPYLAFAAQMMAGLDGIKNRIE
                     PMEPIDKDLYELPPEEAKNIPQVPNSLLDSLEALRNDHDFLLEGGVFTQELIDTWIDY
                     KYEKEILPMAQRPHPYEYELYYGV"
     gene            complement(5520..6437)
                     /locus_tag="VN041_05840"
     CDS             complement(5520..6437)
                     /locus_tag="VN041_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWU28601.1"
                     /translation="METKHRLRRVWDRARWPLVAAIGPIAFGSNYFVTHEYLPAGSPL
                     WGSAIRALPAGLVLLLLARRLPTGAWWWRSVVLGTLNMGLFFCLVYVAAQLLPSSVAA
                     SLMSLSPIVIAGAAWLLIDERPAPRVIVGAVIGGAGVLLIVGTATGALDGWGIAASIA
                     SMLLSSIGAILTRRWQDDTPVLTVTSWQLVVGGLELFAAAVVIEGAPPALTAVEAAAF
                     AYASLIATALGFVCWFTGFRHLPAGVVGVIGLLNPVTGVALGVFAGGEILTPLQAAGI
                     ALVLISIAIVNARRRTAVVLDGERQVVKR"
     gene            6477..6962
                     /locus_tag="VN041_05845"
     CDS             6477..6962
                     /locus_tag="VN041_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU28602.1"
                     /translation="MGSHDRVGTFIEQWRRERPDLDPSPMAVIGRMRRISDDFTTELI
                     RNYRRFGLGEGEFDVLCALRRAGTPFALSQGDLAEHTMVTAGATSKRVDRLEAAGLVI
                     RGQREDDTRGRIVELTPEGRALIDEAYPAHLQTERQLLSSLSDDERSQLERLLRKLVR
                     D"
     gene            complement(7029..8510)
                     /locus_tag="VN041_05850"
     CDS             complement(7029..8510)
                     /locus_tag="VN041_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWU28603.1"
                     /translation="MPSSHPTFGVLAALRSPRQLSREVFAGLVTTLALVPEVISFSVV
                     AGVDPMTSLVASIVLCLTMTFLGGRPGVITAAAGSVALVVAPLVHEHGVKYVLPTVVL
                     AGLLQIVFGVLGLARLMRYVPRSVMLGFVNALGILIFVAQLPHIIGGALPVWGLFALT
                     LAIVLLLPMLTKAVPSPLVAIVVVTAVVIVGRMTVPDVADEGPVGGGLPGLTPLDVPL
                     DLSTLQLILPTAISVALVGLMESLLTAKLVDEITETPSHKGRESWALGVANIAAGFYG
                     GVAGCAMIGQTVLNVKTSGARTRISTFVAGVFMLALVTGLSSLMGQIPMVALAAVMMV
                     VAVTTVDWHSVRPSTLRRMPLSETIVMLVTIGTVVLTSNLAYGVIAGVVLAMILFARR
                     VAHVISVERMPSSDGASVRYAVRGPLFFGSSNDLVEHFRYAEDPASVIIDLSAAQIWD
                     ASTVAALDSIATRYERRGVTIGITGLDARSERFHGRLSGYLGA"
     gene            complement(8582..9010)
                     /locus_tag="VN041_05855"
     CDS             complement(8582..9010)
                     /locus_tag="VN041_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28604.1"
                     /translation="MDGGFDSLHGRWAGEEEVYATAWTPAGRASAELVFAPGPGGTLL
                     IDYAEHRADGDMTGHGVVSDGGWWWFDSYGFVPASAGTSAWEDGELILERRSERGRNV
                     MVLASDGDRLTMRLAAAPVDGELQQLVSGAYRRVRADESV"
     gene            complement(9043..9978)
                     /locus_tag="VN041_05860"
     CDS             complement(9043..9978)
                     /locus_tag="VN041_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWU28605.1"
                     /translation="MSERIGIVWNPTKIDEAVLRDAVSAAFSEADDVQWWETSADDPG
                     RSMAEAAVAAGCDVVIAVGGDGTVRVVAEVIAQQGEGAPALGIVPQGTGNLLARNLGI
                     PLGSVPKALTTIAAGRTREIDMGWVRADDAEAETGFLVMIGSGLDAHMLAETDDDLKD
                     KAGWLAYVEAMGRALTAAETMDFTLRVDDGDEQLLSGHTLLIGNCGAIQGGVTLLPDA
                     VPDDGRLDLLLVSADGAAQWMQTLRTIVWDNGIRRVLAGDEETVSTDAAQHLSGESIR
                     VRLSTPSAFEIDGEEAGEVSSLEVRVQPAALRVLC"
     gene            complement(10000..>12410)
                     /locus_tag="VN041_05865"
     CDS             complement(10000..>12410)
                     /locus_tag="VN041_05865"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HWU28606.1"
                     /translation="VARATVTDAGTPRAEVSATRLAIIGMGKAGARELNYLSDVDVIF
                     VAGTGDEQIVSEPRAIDVATRLARETMRVLSGIDAEPPLWEVDAALRPEGKQGALVRS
                     LASHEAYYDRWAKSWEFQALLKARPLAGDAELGAEYIAAVQPRVWTSAERSDFVDSVQ
                     RMRERVTDNIPAEDVPYQLKLGPGGLRDIEFTVQLLQLVHGVSDPSLHTRGTLESLEA
                     LAEGGYIGRAEGAAFAEQYRVLRLLEHRLQLHELTRTHLMPRDDEGMRRLARATGLAD
                     SGPALRQLWENLRRDVREQHVRLFYRPLLAAVASLPAEERALTTGQAADRLAAIGFRD
                     PAGALRHIAALTTGLSRKASIQRYLMPVMLRWFADGTDPDYGLIAFRRISERLGDTHW
                     FLGMLRDSSGAAERLTRVLSSSRYVGELMEWIPESVAWLDSASDLTLRSGAALDEEAR
                     AIQTRHATIGPAMKAVRALRRRELLRTAMGAVLDHVGVHEMARAISEISEATIQAALR
                     AVRREVVPPEDDALDFSIIAMGRFGGAELGFGSDADVLFVYEPNGVEPQRAQQLAASI
                     VAGLREHLTDHRLPLDLDADLRPEGRNGPIVRTLEAYTQYYRRWSLSWEAQALLRARG
                     IAGSVKLIGRFMELADGIRFPEQVSLQDVREIKRIKARVEGERLPQGVDPRRHLKLGP
                     GTLSDVEWMVQVLQLQHGHRVPDLRTTSTLQALDAAVLADLLPAGAAERLREAWVLCS
                     RLRSAMTLLTGQTRDVLPVDHRELDGVGRLLGYPDRGAMLLEEDYLRVTRRARRVFET
                     HFYG"
BASE COUNT         2028 a         4498 c         4099 g         1785 t
ORIGIN      
        1 tcgagtgcgg ccgtccgctc cggcgtccac gccggaggac accttcggct tcgacggcat
       61 gctctcgcgg aggcgctcct gcagacgtgc gccgatcagc acgagccgct cacggagcga
      121 gcgccgctcc tccagttgcc cggccacctc ggccaggccg gcacggtgga agggcagggc
      181 gcgcacccgc tccggggtcg gccatgggcc gtccatgtcg ggtacctcgc gctcgagggc
      241 acgcggggcg gcgagctcgg tcagttcgcc ctcccaggcg tcgagctgac gcagcaccat
      301 cctgtgatcc gacattcccg cagcgatccg caccagcgtc cgctgcatcg cgcgatccgc
      361 gcactcgtct ctgacgcgct cgacgactgc caccgatgcg gccgtgctgg acaccccctc
      421 acccgggacg aacaccggac gtccgccgtc ctcgagccac cagcggccgg tgaccgcccg
      481 atccgccacc tcgacgatcc cgcgcatcag gctgccgacg agagtcacga tctcgccgga
      541 cgtcaacgga cgtccggcgg cgcgccggcg ggcgatcagc gtctcgaccg gttcgacgca
      601 ccagggaagc agtgcgccgt gaccgtcggc ggtccgcacg agatcatgca cgccggcgac
      661 gtgctccgcg cccgcgaact cccacagcgc ggcgtccgtc gctgcggtgt cgaccagcac
      721 cctccgatgt tcaccgtcgc tgacgagctc cccggttaac ggcgactccg tgccgtcgag
      781 ccggcggatg aggcgatgcg ctccgggcac gagtccgagc cggggctccg gatgctgctc
      841 ctcgacgttg tccatcgctc catgagagcg catgcctgcg acccccaagg gcccggatca
      901 cgacgaaggt ggagaagtca cgggataccc gcggctgtgc agggagaacg acggggcgat
      961 gactcggtag gctggtaccc atggcaaagc gtgctcccga agctgagaag cgtccaggct
     1021 tcttctccca gatccgttcc ctgttccggt tcacccggga ggtctacggc tggcttccct
     1081 gggcgcagct ggccatcctc atcggcggcg tgctcatcgg cctgatcctc ggcttcctgg
     1141 tcggcggcgg cgccgtgctc tcgctgatcc tgtgggccgt caccggcctg atgttcggcg
     1201 tcctcggcgc catgttcctg atgacgcgcc tgtcgaccaa ggccatgtac gccaagatcg
     1261 acgggatgcc cggcgcgagc ggtcacgtgc tcagcaccag cctcggccgc agctggcagg
     1321 catccgacag ccccgtgggg atcaacccca agacccagga ggccgtctac cgcgccatcg
     1381 gccgcggcgg catcgtcgtg gtcggcgagg gcacccgtgg tcgactcacc cgcctcgtga
     1441 acgaggagcg tcagaaggcg cagcgcgtcg ccaacggcgt gcccgtgacc gtgctgtacg
     1501 tcggccacgg cgaggacgag gtcccgatct ccgacctggc caagcggatc aagaagatcc
     1561 ccaaggcgat cgacaaggcc accatggccg ccgtcatccg acgcatcgac tcggtgtcgc
     1621 agtcgctctc gtcgctgccg atccccaagg gcatcgatcc caccaaggtc cgcgcgcagc
     1681 gtccgcgctg atccgcgcca ctgcacgcag cggaacggcc cctcactcga tcggagcgag
     1741 gggccgttcc gcgttcactc ccccagcggc tccttgggga ggcggcgcac acgcggccgg
     1801 cggcgacgct tctccgggat catcgatcgc atctcctcga gctttccgaa gcacagcagc
     1861 cggtcctcgg cctcgagcac cacatgcttg cgcgggttgg ggatgacgct cacgccgcga
     1921 tgcagggtga gcaccgtgat gtcgcggtcc cacagcccca ggtcgcccag cttcatgccg
     1981 acctgctccg caccgctgtg caccatgagc tcggcgacgc cgtagccggt ggagacgctc
     2041 agccgctgac ggatgtcgat gtcggggaac gccacctggt tcgcgatgta gtcgatgatc
     2101 gcgccggcga catcgaggtt ggtggccgcc tcgatgccct gcagtccggg cgaggagttg
     2161 acctccatga ccagcggccc ctcgtcgctc tcgagcatgt cgacaccggc gacccgcaga
     2221 cccatgatct gggccgagcg gaccgcggtc tgctcgtaca ccgggtccag cgtcaccggc
     2281 tccacgcgcc cgccgcggtg cacgttggag cggaactcgt cgccctcggc gacgcgccgc
     2341 atcgcggcca cgacccggtc tccgacgacc agagcgcgga tgtcgcgtcc ccggctctcg
     2401 gcgatgaact tctggatcag cacgttctgc ttggtggagt gcagcgtctc gatgatcgcc
     2461 tcggccacct tcacctgcgg agcgaggatc acaccgatcc cctgggtgcc ctccagcagc
     2521 ttgatcacga cgggggcgcc gcccacgcgc tcgatcgcag gacgcacgtc cgcgcggttg
     2581 cgcacgaacg ccgtggccgg cattgcgatg ttgtggcggg acaggatctg gttggcccgc
     2641 agcttgtcgc gcgcgctgga gatgccgttc gcggtgttcg gggtgtagac gtccatctgc
     2701 tcgaactgac gcaccaccgc cgtcccgaag tacgtgatgg agttgccgat gcggggcagg
     2761 atcgcgtcgt agtcgctgag ctgcctgccg cggaagaaca ggtccggggc gtcagcggtg
     2821 aggtcgatcg cgaaccgcag ggtgttcagc accttcactg tgtggccccg ctgctgcgcg
     2881 gccgcgcgaa ggcgctgggt ggagtacgac tgcggtgccc gcgagagaac ggcgatcttc
     2941 acagatgttt cctgccagga tgtaggggtg aatcggtctt ctcattcaaa cacgtccacg
     3001 gggtggcgcg agtgggtgtc cctgcccgag ctgggtgtcg actggatcaa ggccaagatc
     3061 gacaccggcg cgaggacctc gtcgctgcac gcgttcgacg tcgaggagtt cacccgtgac
     3121 ggcgaggact gggtgaggtt cgccgtgcat ccctggcagg agaccaggga ggatgccgtg
     3181 gtgcacgagt gccccgtcca cgatcgccgc gccgtgcgca gctcgtccgg ccacgctgag
     3241 caccggatcg tcgtgctgct gcggatgcgg ctggtcggcc acgacgtgca gggcgaggtg
     3301 acgctcacca atcgcgacga gatgggcttc cggatgctca tcggccgcca ggtgctgcgt
     3361 cgcggcttcg tcgtcgatcc cgccaagtcg ttcctgggcg gtcgtgcccc gcgcgagatg
     3421 cgccggagga accgcgggcg cgactgagcc catcgcgccc ggtcgcgggc gggaccgagc
     3481 gcgtccggat caggaccgga tgagaaccgt gccggcgatc ttgtcgtgca gaccgcgctg
     3541 atcggcatcc cagatgacgg cggggatgac gaccaccagc aggagcgtgc ggacgatcgg
     3601 acgccacagc ccgacccagc cgcctccgaa gcggaccacg cgcatgccga ggatgcgatg
     3661 ccccgggctg ccaccggcgg tcgggatgaa caggatctgc agcagcgcga agaccatcat
     3721 cggcgcgaac tggcgccagc cggcctccgc gggcagagcc agcgggtcgt agccgaggaa
     3781 gccgagcgcg atgagcgtcg aggcgaagta gtcgatcgcc aatgcgccga tcctgcggcc
     3841 gggacgggcg atgctgcggg gtccggtggc gggcagaccg aggcgttcac cggggtagtc
     3901 gttcactccc ccagcctacc gagcacacgt aacatgcccg aaacatggcg gacacggctg
     3961 ggaaactccc aatgcataac ctgcgtaagg ttgatgcaac cgatacccga tcaggagtcg
     4021 tacatgttca aagagcccgc agacgtcctt cgctacatcg aggagaacga cgtcaagttc
     4081 ctcgacatcc gtttcaccga ccttccgggc gtgcagcagc acttcaacat cccggcatcc
     4141 acggtcgacg aggacttctt ccgggacgga cagctgttcg acggctcctc gatccgcggg
     4201 ttcgcgtcga tccacgaatc ggacatgcag ctgatccccg atgtcaccac cgcgtacctc
     4261 gacccgttcc gcgaggcgaa gaccctcgtg atggtcttcg acatctacaa cccgcgcacc
     4321 ggcgagatct acagcaagga cccgcgtcag gtcgccaaga aggccgagaa gtacctcgcg
     4381 tccaccggca tcgcggacac cgcgtacttc gcccccgagg cggagttcta catcttcgac
     4441 gacgtgcgct actccgtcac cgcgggcgag agcttctacc ggatcgactc cgaggaggcc
     4501 gcctggaact ccggtcgcga ggaggaaggc ggcaacctcg ccaacaagac ccccttcaag
     4561 ggcgggtact tcccggtcgc tcctgtcgac aagacggccg acctgcgcga cgacatgacg
     4621 ctgcgcctga tcgacgcggg cttccacctg gagcgctcgc accacgaggt cggcaccgcg
     4681 ggccagcagg agatcaacta ccgcttcgac accatggtga gcgcggccga cgacatcctg
     4741 aagttcaagt acatcatcaa gaacaccgcc caggagtggg gcaagacggt cacgttcatg
     4801 ccgaagccgc tgtatggcga caacggctcg ggcatgcaca cgcaccagtc cctgtggctg
     4861 gacggcaagc cgctgttctt cgacgaggcc ggctacggcc agctcagcga catcgcccgc
     4921 tggtacatcg gcggtctgct caagcacgcg cacgccgtgc tggccttcac caacccgacg
     4981 ctgaacagct accaccgcct ggtgaagcac ttcgaggccc cggtcaacct ggcgtactcc
     5041 gccggaaacc gctccgccgc gatccgcatc ccgctgaccg gctcgaaccc gaaggccaag
     5101 cgcatcgagt tccgcgtgcc ggatgcctcg ggcaacccgt acctggcctt cgctgcgcag
     5161 atgatggccg gcctggacgg catcaagaac cgcatcgagc cgatggagcc catcgacaag
     5221 gacctgtacg agcttccccc ggaggaggcc aagaacatcc cgcaggtgcc gaactccctg
     5281 ctggactcgc tcgaggcgct ccgcaacgac cacgacttcc tgctcgaggg cggcgtgttc
     5341 acgcaggagc tcatcgacac ctggatcgac tacaagtacg agaaggagat cctgccgatg
     5401 gcgcagcgtc cgcacccgta cgagtacgag ctgtactacg gcgtctgagt cgcgcccctc
     5461 tggctcgagg gcctgacctt cctgggttct tcccggaggt caggccctcg aggcgtttat
     5521 cacctcttca cgacctgtcg ctctccgtcc aggacgacag cggtgcggcg tcgggcgttg
     5581 acgatcgcga tgctgatcag cacgagcgcg atgcccgccg cctggagcgg tgtgaggatc
     5641 tcgccgccgg cgaagacccc gagtgcgacc ccggtgacgg gattgagcag accgatcaca
     5701 ccgacgactc ccgccggcag gtggcggaac cccgtgaacc agcagacgaa gccgagggcc
     5761 gtcgcgatca ggctcgcgta ggcgaacgcg gccgcctcga cagcggtgag ggcgggaggc
     5821 gcgccttcga tcacgaccgc ggccgcgaac agctcgagtc cgccgaccac cagctgccac
     5881 gaggtcaccg tgagcaccgg agtgtcgtcc tgccagcgcc gggtgaggat cgcgccgatc
     5941 gacgagagca gcatcgaggc gatcgacgcc gcgatccccc agccgtccag ggctccggtc
     6001 gccgttccga cgatcagcag cacgccggca cctccgatca ccgcgccgac gatcacgcgc
     6061 ggtgcagggc gctcgtcgat cagcagccag gccgctcccg cgatcacgat ggggctcagc
     6121 gacatcagcg acgcggcgac actcgacgga agcaactgag ccgccacgta caccaggcag
     6181 aagaacaacc ccatgttgag cgtgccgagc acgacggaac gccaccacca cgcccccgtc
     6241 ggcaggcgtc gcgcgaggag cagaagaacc agcccggccg gcagcgcgcg gatcgccgac
     6301 ccccacagcg gcgagccggc cggaaggtac tcgtgcgtca cgaagtagtt cgacccgaac
     6361 gcgatcgggc cgatcgccgc gacgagcggc caccgggccc tgtcccacac acgtcgcagc
     6421 cggtgcttag tttccatgga aaccattata tgttccgcgg aaactatgct ggatgcatgg
     6481 gcagtcacga ccgagtagga accttcatcg agcagtggcg tcgcgagcgc ccggatctcg
     6541 atccgagccc gatggccgtc atcggcagga tgcgccgcat ctcggacgac ttcacgactg
     6601 aactgatcag gaactatcgc cgcttcggcc tgggcgaggg cgagttcgac gtcctctgcg
     6661 ctctccgcag ggccggaacg cccttcgcgc tctcccaggg cgatctcgcc gagcacacga
     6721 tggtgacggc gggagccacc tccaagcgcg tcgatcggct cgaagccgcc ggtctcgtga
     6781 tccgcggtca gcgcgaagac gacacccgcg gtcgcatcgt cgagctcacg ccggagggac
     6841 gcgcactcat cgatgaggcc tatcctgcgc atctgcagac ggagcgccag ttgctctcct
     6901 ctctcagcga cgacgaacga tcgcagctcg agcgcctgct ccgcaagctc gtgcgcgact
     6961 gagctccccc actcccccga cgggcgcggc ggccgcctgc ggcagcgcag ggctgccgcc
     7021 cgataccctc aggcgcccag gtagccgctc aggcggccgt ggaagcgctc gctgcgggcg
     7081 tcgaggccgg tgatgccgat cgtgacccca cggcgctcgt agcgcgtcgc gatcgagtcc
     7141 agggcggcca cggtcgaggc gtcccagatc tgcgcagcgc tcaggtcgat gatgacggat
     7201 gccggatcct ccgcgtaccg gaagtgctcg accagatcgt tgctgctgcc gaagaacagc
     7261 ggtccgcgca cggcatagcg cacgctcgcg ccgtcggagg acggcatccg ctccacgctg
     7321 atcacgtgcg ccacgcggcg cgcgaagagg atcatcgcca ggaccacccc ggcgatgacg
     7381 ccgtaggcga ggttgctcgt cagcacgacg gtcccgatgg tgacgagcat gacgatcgtc
     7441 tcggagagcg gcatccgccg cagcgtcgac ggacgcacgc tgtgccagtc caccgtggtg
     7501 acggcgacga ccatcatcac cgcagccagc gccaccatcg ggatctgccc catcagggag
     7561 ctgagcccgg tgacgagggc cagcatgaac acgcctgcaa cgaaggtgga gatgcgcgtc
     7621 cgcgcaccgc tggtcttcac gttgagcacg gtctggccga tcatggcgca tcccgcgacg
     7681 ccgccgtaga agcccgcggc gatgttcgcg acgccgagcg cccacgactc ccgacccttg
     7741 tgcgagggag tctcggtgat ctcgtccacg agcttcgccg tcagcagcga ctccatcagc
     7801 ccgaccagcg ccacgctgat cgcggtcggc aggatcagct gcagggtcga gagatccagc
     7861 gggacgtcca gcggggtgag gcccggcaga ccgccgccca cgggaccctc gtcggcgacg
     7921 tcgggcaccg tcatccggcc gacgatgacg accgcggtga cgaccacgat cgcgaccagc
     7981 ggcgagggaa ccgccttggt cagcatcggc agcaggagca cgatcgccag agtcagggcg
     8041 aacagacccc acaccggaag ggccccgccg atgatgtgcg gcagctgcgc gacgaagatc
     8101 aggatgccga gcgcattgac gaatccgagc atgaccgacc tcggcacgta ccgcatcagc
     8161 cgtgccagcc ccaggacccc gaagacgatc tgcaggaggc ccgccaggac gacggtcggc
     8221 agcacgtact tcacgccgtg ctcgtgcacg agcggcgcga cgaccaacgc gaccgagccg
     8281 gccgcggccg tgatcacgcc gggccgcccg ccgaggaacg tcatcgtcag gcagagcacg
     8341 atcgacgcca ccaggctcgt catcgggtcg acgccggcga ccaccgagaa cgagatcacc
     8401 tcgggcacca gagcgagggt cgtcaccagc ccggcgaaca cctcgcgcga gagctgccgc
     8461 gggctgcgca gtgcggcgag cacgccgaac gtcgggtgcg atgagggcat cgggactcct
     8521 gcctgagggg acgcccgatt ctacccgcgt cggcgggcgc ctccggcacc cgaagacgac
     8581 ctcagaccga ctcgtccgcg cgcacgcgac ggtacgcacc cgagacgagc tgctggagct
     8641 ccccgtccac cggtgcggcc gcgagacgca tcgtgagccg gtcgccgtcg gatgccagga
     8701 ccatcacgtt gcgcccacgc tccgagcggc gctccaggat cagttcgccg tcctcccacg
     8761 cggacgtccc cgccgaggcg ggcacgaacc cgtacgaatc gaaccaccac cagccgccat
     8821 ccgagaccac gccgtggccg gtcatgtccc cgtcggcgcg atgctccgcg tagtcgatga
     8881 gcagggtgcc tccgggcccc ggtgcgaaga ccagctcggc cgacgcacgg ccggccggcg
     8941 tccacgccgt cgcgtacacc tcctcctcgc ccgcccatcg cccgtggaga ctgtcgaagc
     9001 cgccgtccat cctgcgcccg tccttccctc gacccgtcgc cctcagcaca gcactcggag
     9061 cgccgccggc tgcacgcgca cctcgagcga cgagacctca ccggcctcct cgccgtcgat
     9121 ctcgaacgcc gaaggcgtgg agagccggac gcggatgctc tcaccggaga ggtgctgcgc
     9181 ggcatccgta ctgaccgtct cctcgtctcc cgcgaggaca cgccggatgc cgttgtccca
     9241 gacgatcgtg cgaagggtct gcatccactg cgccgcaccg tcggcgctca cgagcagcag
     9301 atcgagccgg ccgtcatcgg gcacggcatc gggcagcaga gtcaccccgc cctggatcgc
     9361 gccgcagttg ccgatcagca gggtgtgacc gctgagcagc tgctcgtccc cgtcatcgac
     9421 gcggagcgtg aagtccatcg tctcggctgc ggtcagggcg cgccccatcg cctcgacgta
     9481 ggccagccag cccgccttgt ccttcaggtc atcgtcggtc tcggcgagca tgtgcgcgtc
     9541 caggcccgac ccgatcatca ccaggaagcc ggtctcggcc tcggcgtcgt cggcacgcac
     9601 ccagcccatg tcgatctcgc gcgtgcggcc tgcggcgatc gtggtgagcg ccttcggcac
     9661 gctcccgagc gggatgccga gattgcgggc gagcaggttg ccggtgcctt gcgggacgat
     9721 gcccagtgcg ggcgcgcctt caccctgctg cgcgatgacc tccgccacca cccgaaccgt
     9781 tccgtcaccg ccgaccgcga tgaccacgtc gcatccggcc gccaccgcgg cttcggccat
     9841 ggagcgcccg gggtcgtccg ccgacgtctc ccaccactgc acgtcgtcag cctcggagaa
     9901 cgccgcgctg acggcatccc tcagcactgc ctcatcgatc ttggtcgggt tccacacgat
     9961 cccgatgcgc tcagacatga ctccccctcg tgttctttct cagccgtaga aatgggtctc
    10021 gaagacccgc cgggcgcgac gcgtgacccg cagatagtcc tcctcgagca gcatcgcgcc
    10081 gcggtcaggg taacccagca ggcgcccgac cccgtccaac tcgcggtgat cgaccggcag
    10141 cacatcacgg gtctggccgg tcagcagcgt catcgccgag cgcagccggc tgcacagcac
    10201 ccaggcctcg cgcagcctct ccgcggcacc ggccggcagc agatcggcga gcacggcggc
    10261 gtccagcgcc tgcagcgtgg atgtcgtccg caggtcgggc acccggtggc cgtgctgaag
    10321 ctgcagcacc tgcaccatcc actccacgtc gctgagggtc ccgggaccga gcttgagatg
    10381 cctgcgcgga tccacgccct gcggcagtcg ctcgccctcc acccgcgcct tgatgcgctt
    10441 gatctcgcgc acgtcctgca gcgagacctg ctccgggaag cggatgccgt cggcgagctc
    10501 catgaaccgg ccgatgagct tcacgctgcc ggcgatgccg cgggcgcgca gcagcgcctg
    10561 cgcctcccag gacagggacc agcgccggta gtactgcgtg tacgcctcca gcgtccggac
    10621 gatcggtccg ttgcgcccct ccgggcgcag gtcggcgtcc agatcgagcg gcagcctgtg
    10681 atcggtcagg tgctcccgca gacccgcgac gatgctcgcg gccagctgct gggcccgctg
    10741 cggctcgacg ccgttcggct catagacgaa cagcacatcg gcatccgatc cgaagccgag
    10801 ctcggccccg ccgaaacggc ccatcgcgat gatcgagaag tccagcgcgt cgtcctcggg
    10861 cggcacgacc tcgcgccgca ccgcgcgcag cgctgcctgg atcgtggcct cgctgatctc
    10921 gctgatggct cgcgccatct cgtgcacgcc gacgtggtcg agcaccgcgc ccatggcggt
    10981 gcgcagcagc tccctgcggc gcagcgcccg tacggccttc atcgcgggac cgatcgtggc
    11041 gtgccgggtc tggatggccc gcgcctcctc gtccagcgcc gccccgctgc gcagggtgag
    11101 gtcgctcgcg gagtccagcc acgccaccga ttccgggatc cactccatca gctcgccgac
    11161 gtagcgcgag ctggagagca cgcgggtgag ccgttcagcg gccccggacg agtcgcgcag
    11221 catccccagg aaccagtgcg tgtcgccgag gcgctcgctg atccggcgga aggcgatcag
    11281 cccgtagtcg gggtcggtgc cgtctgcgaa ccagcgcagc atcaccggca tgaggtaccg
    11341 ctggatgctg gccttgcggc tgaggcccgt ggtcagcgcc gcgatgtgcc gcagcgcgcc
    11401 ggccggatcg cggaagccga tggccgcaag acgatctgcg gcctgcccgg tggtgagcgc
    11461 ccgctcctcg gcgggcagcg aggccaccgc cgcgagcagc ggccggtaga acagccggac
    11521 atgctgctcc cgcacgtcgc gccgcagatt ctcccagagc tggcgcaggg cggggccaga
    11581 gtcggcgagc cccgtggccc gggccagtct gcgcatgccc tcgtcgtcgc ggggcatcag
    11641 atgggttcgg gtcagttcgt gcagctgcag gcggtgctcg agcaggcgca gcacccgata
    11701 ctgctcggcg aacgcagcgc cctcggcccg cccgatgtag ccgccttcgg ccagtgcctc
    11761 cagcgactcc agcgtgccgc gcgtgtgcag gctcgggtcg ctgacgccgt gcaccagctg
    11821 cagcagctgc accgtgaact cgatgtcgcg cagaccgccc gggccgagct tgagctggta
    11881 cggcacatcc tcggcgggga tgttgtccgt gacgcgctcg cgcatgcgct ggacgctgtc
    11941 cacgaagtcc gatctctcgg cgctggtcca cacgcgcggc tgcacggcag cgatgtactc
    12001 ggcgcccagc tccgcgtctc ctgcgagcgg acgcgccttc agcagcgcct ggaactccca
    12061 gctcttcgcc caccggtcgt aataggcctc atgcgaggcc agggagcgca ccagcgcgcc
    12121 ctgtttgccc tccggccgca gcgcggcgtc cacctcccag agcggcggct ccgcgtcgat
    12181 gccggagagc acgcgcatgg tctcgcgggc gagcctggtc gccacgtcga tggcgcgcgg
    12241 ctcgctcacg atctgctcat cgcccgtgcc ggcgacgaag atcacgtcca cgtcgctgag
    12301 gtagttcagc tctcgcgcgc cggccttgcc catcccgatg atcgccagcc gggtcgccga
    12361 gacctccgct cgcggtgtcc cggcgtccgt cacggtcgcg cgggcgacgg
//
LOCUS       DATMSX010000191        10801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_21416,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000191 DATMSX010000000
VERSION     DATMSX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21416"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VN041_05870"
     CDS             <1..772
                     /locus_tag="VN041_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU28607.1"
                     /translation="RAVQRGRLRRLLEEAGLARTSTRTLVVTMVGVGLVAAALAWLVT
                     GLPVLALLAGLAAGSAPVLYLRGRRLRLRKARRQLWPDVCDLLVAAIRVGLSLPDAVA
                     SLADSAPSAVRPAFAVFARDLRATGRFETSLDRLKATLADPIADRIIETLRMARQVGG
                     TELTGVLRALSSSVRADAALRGEVEARQSWIRGAAVLGAVAPWVILGLLVSRPEGREA
                     YASAEGVIVIVVGAAVSLVAYRIMLRIGRLAEPERWFG"
     gene            769..1707
                     /locus_tag="VN041_05875"
     CDS             769..1707
                     /locus_tag="VN041_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWU28608.1"
                     /translation="MSAVTDLALAVLLGGAFALGVLGVLSALPRWGALPLDRRIAPYL
                     RDVVPDEAMPSGILPRVGMLPVHGRTVWERVKSAFERMLGGGDALALRLAQAGSPLSP
                     AVFRGRQLAWGVTGLVAGALLVVLIAVAGRMTPPAVLLPLLSAAGVAVAYDMQLTGRA
                     KSRRARLADELPTTLEFLALCLSAGESLLDALRRVSGIGTGELTEELRHAVLAVHTGS
                     PLADALGETATRLQLPGLTRAVDQIIAALEHGAPLAAVLHSQAADARDEAKRVLIEQA
                     GHKEILMLLPLVFLILPLSVLFAVYPGLFILRLGLG"
     gene            2129..2293
                     /locus_tag="VN041_05880"
     CDS             2129..2293
                     /locus_tag="VN041_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28609.1"
                     /translation="MEDLDWGAQPRCPEDGILMRDVPGGWECPHCGHVQQAQDVEPPP
                     EFDGPSIWGG"
     gene            complement(2290..2490)
                     /locus_tag="VN041_05885"
     CDS             complement(2290..2490)
                     /locus_tag="VN041_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28610.1"
                     /translation="MDRDKLKFAFWLVGSCGVLAVTLAVIDFYVDPASGANLSGIIDM
                     LKLVATTALGFVFGRSLGKGDH"
     gene            complement(2595..3071)
                     /locus_tag="VN041_05890"
     CDS             complement(2595..3071)
                     /locus_tag="VN041_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007051890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HWU28611.1"
                     /translation="MVFSAVNVSNNILKRAFEEKVSVSPMKLQKILYFVASEYAKRTG
                     RPLLSEGFQPWRYGPVVRTVYDEFRSFGGGEIRAFGKDAQGKAYMADESKDAALSAAL
                     SDVWRVTKPVSAVELSRITHLAGSAWSQHSGSYGYLSDAAVLGDKTYLEPLGLVER"
     gene            complement(3178..3345)
                     /locus_tag="VN041_05895"
     CDS             complement(3178..3345)
                     /locus_tag="VN041_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28612.1"
                     /translation="MARIHDTWMRLEDGAIITLHGLLGDQIIWAYRGDETAMFTHHEA
                     FVRDHRQVTLH"
     gene            complement(3388..3651)
                     /locus_tag="VN041_05900"
     CDS             complement(3388..3651)
                     /locus_tag="VN041_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28613.1"
                     /translation="MTAINIEHTVTIWMVNDIPARMVYAGRRWRVTDTPTRIRHSIWT
                     VPLDEPHHGLYGWRFQGTDDDGLSLVFDVYRGEDGWHVHRAYD"
     gene            3725..4078
                     /locus_tag="VN041_05905"
     CDS             3725..4078
                     /locus_tag="VN041_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28614.1"
                     /translation="MGTNKRYADSIDRRANERHILDLSRTAPLQSLTEKELQLDIYPL
                     TTYPRPQRCRVWVRFGPHPFRVEGRVLRTTQLAAGVEFKLEETTFKCWVWGPACEVIT
                     LDDVIADRKAHGGLA"
     gene            4109..4804
                     /locus_tag="VN041_05910"
     CDS             4109..4804
                     /locus_tag="VN041_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWU28615.1"
                     /translation="MCGRFAMDAKTDELIQQFVADGGKPEDWWKDFHGSYSIAPTDDA
                     VIVRDRGEGRSLELVRWDWQKPANRPKGAPIINARIEKLDTGFWLPAFTAARCVVPMR
                     GYFEWTGEKGDKTPHFLHGDGLLAAAGLTWSMDLPSGERSRCMVVVTREARDAGGEVH
                     DRMPAFLTEDAMAAWLEPVKLDRGDRDGMLSLLDAASADVASTIRQYVVDRKVNNSRT
                     VDTGDQSVIAPAA"
     gene            4815..5057
                     /locus_tag="VN041_05915"
     CDS             4815..5057
                     /locus_tag="VN041_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28616.1"
                     /translation="MLTGQIRPVKTRILDVEGTHDEIVAALNAAAVDGWEVDSITTRR
                     LAHYGATTTVEAADIDAVRAKVPDGHQLLAVTSSGL"
     gene            5128..5625
                     /locus_tag="VN041_05920"
     CDS             5128..5625
                     /locus_tag="VN041_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28617.1"
                     /translation="MKKLSTIAQALAEEIAAQDWSDAHNRADGARHDRAADHTTTTQL
                     SPTEAEFVRVNVMWVTAQALSAHEANFDVHAFAEAAGVDPRFRLRSDGSPSGAIPAGL
                     RWSDQTYKPADLARLLGYRDEARPGKVVRDYLRKTYPDHQKHKPWLLSEEEAADVLAN
                     VPRKR"
     gene            complement(5694..6134)
                     /locus_tag="VN041_05925"
     CDS             complement(5694..6134)
                     /locus_tag="VN041_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28618.1"
                     /translation="MDIILPTIPAGVLVLLALLAPYVQALIQRPSWSPIVKKVAAVAV
                     ALALTGVVLGFYYMYTGDTVPEWPALALLAIVVAQASYTMVTKSSATALELRTSRDVV
                     LSQDEDGTWRDPAVRDRVLADFAKLDPDEQNTTMQNAIAVTSGE"
     gene            complement(6134..6460)
                     /locus_tag="VN041_05930"
     CDS             complement(6134..6460)
                     /locus_tag="VN041_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28619.1"
                     /translation="MTMEAAQLITIILSSSVVSGIVLKLFDLIKDARAGQLQQRRAEV
                     DRAETAKDKALARAEAAEAAEEAQARRTRIVEESLAVHRRLIIDAPCLGPGTLPDYPS
                     RSERNL"
     gene            complement(6457..6825)
                     /locus_tag="VN041_05935"
     CDS             complement(6457..6825)
                     /locus_tag="VN041_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28620.1"
                     /translation="MKVTYAAIYALVLATGIATLLGPPRSIEGELGSVLTVIWSVFLI
                     VGGTAGALTVLPGWWWAERLSLILTMTGITIYGLVVLSLHLTQEGSRLTQLGMILLAM
                     SVFAVRWMLIRHYSFEPRRG"
     gene            complement(6873..7016)
                     /locus_tag="VN041_05940"
     CDS             complement(6873..7016)
                     /locus_tag="VN041_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28621.1"
                     /translation="MTRGTNIQHGQARRHPIPLREWIGLGVLLAVGLAALTLMCAPSW
                     GWW"
     gene            complement(7013..7963)
                     /locus_tag="VN041_05945"
     CDS             complement(7013..7963)
                     /locus_tag="VN041_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HWU28622.1"
                     /translation="MTKGTVSGIAGADRMMSWRTYGSGLCLQAVDRALQKPLSDRPGF
                     YTYALRALETVPAANRHYDRTPPKGAIVFFSAGRNGYGHICISVGGGNVVSTDIPSSG
                     RVGVTSISAIEKAWGRKFLCWTNWVMGYNVTTATPAAPAPAGGASGSYPARKLYGGGW
                     VTFVQDLLAILGHDTGGRDGKDGTKTQAAVKHEQGAAKSNGYGTLTQDGIAGPASLTY
                     LLWAVQKFRTKGEKTAGQPARTRYGQAWVKLIQRMLVELGHPLTVDGADGAKTQAAVR
                     HEQGAAKSNGYGTLTQDGIAGFATAKYLVWAIAKYRLPWT"
     gene            complement(8118..8429)
                     /locus_tag="VN041_05950"
     CDS             complement(8118..8429)
                     /locus_tag="VN041_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28623.1"
                     /translation="MATTTQHINARTDPDLLARFVAAAEQADVPDASAWVQANMGRLV
                     CITIDGTQTVSDVHAYAATAREQYVAATPPRPGENLGAVTDTHLAVAITALRPAPEPE
                     A"
     gene            complement(8441..10444)
                     /locus_tag="VN041_05955"
     CDS             complement(8441..10444)
                     /locus_tag="VN041_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF859 family phage minor structural protein"
                     /protein_id="HWU28624.1"
                     /translation="MTVRLTFNETGPITQFLLDADYIGMSIDGNYSIVDVRIRNINTG
                     SGTTSWYGGAGTHVGSIDGVGEFARWQVSSNFLNKVNSNVTRWDIYKRVYIPHNPDGT
                     RSPAVIRMSVAYGNINHGDAYSMALPTIPRATTPRFRVGSTDVTTFDAGAAVTVFLDR
                     ASSSFTHDVSFQFGNQSGTIATGAGVSAAWTPPMSLLTEIPNNTSGTGTVTVVTKNGS
                     TVIGTKQKNFTLTAPATVVPTISGINVSDDNPDVANLIGGLVQGQSRLKADVIAAGAY
                     GSTISSRMVTVDGQTVTSGQSLPLPQSGSRVVAAAVTDSRGRTAADSGTVTVLPYDLP
                     QIIDFSVQRATAAGVPSSTGTNLLMTLNAAVSSLVVGSEKNAATITVATRPRGGSAWT
                     DRNVITPGLTYNTSVLVSGGGIFMTTTAWDVRVTVADKLQSAQDFYLAPTAGAILDAT
                     PTKVAIGKMVEDAGPALQLQGPARVYDGDLEADLELRHRGGYAVEPVGILVAFAGATA
                     PGGWLLCDGAAVSRTTYAALFAVIGTVYGAGNGSSTFNVPDLRGRIPVGRDSGQTEFD
                     VLGETGGEKAHKLTPSEMPTHNHGAPSGFFYVESPTGGMRRATNLNWAGTSGEDGVWR
                     QRIAETSGGSGGGQPLGTANAGGDGVHNNLQPYVVTNHIIKAF"
     gene            complement(10505..>10801)
                     /locus_tag="VN041_05960"
     CDS             complement(10505..>10801)
                     /locus_tag="VN041_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28625.1"
                     /translation="RHQHGLFGAQAQAAAAQSGVDALQLAVQITPQDVRISRPGAPVA
                     LHLGNDQASFRRNGVAQTWWDENQMIVPKLKASQVVVGQTVITEGAGRTTWQRL"
BASE COUNT         1650 a         3558 c         3787 g         1806 t
ORIGIN      
        1 gcgggcggtg cagcgcggtc gattgcgacg gcttctggag gaggcgggac tcgcacgcac
       61 ctcgacgcgc acgctcgtcg tgacgatggt cggggtcggg ctggtggccg cggcccttgc
      121 ttggttggtc accggcctgc cggtgctcgc gctcctggcc gggcttgcgg ccggttccgc
      181 tcccgtcctg tatctgcgcg gacgccgtct tcgtctgcgc aaggcgcgcc gccagctgtg
      241 gccggacgtc tgcgatctgc tggtggctgc gatccgggtc ggactctcgc tgcccgacgc
      301 cgtggcgagc ctcgccgact ccgcgccttc ggccgttcgt cccgcgttcg cggtcttcgc
      361 ccgtgatctg cgcgcgaccg gacgcttcga gaccagcctg gatcggctga aggcgacgct
      421 cgccgatccg atcgccgacc gcatcatcga gacgctgcgg atggccaggc aggtgggtgg
      481 caccgagctg accggggtgc tgcgggctct ctcctcgtcg gtgcgggcgg atgccgcgtt
      541 gcgcggcgag gtggaggcgc ggcagtcctg gatccgcgga gccgcggtgc tcggcgcggt
      601 cgccccctgg gtgatcctcg ggctgctggt gtcccgcccg gagggacggg aggcatacgc
      661 cagtgcggag ggcgtgatcg tgatcgtcgt gggcgccgcg gtgtcgctcg tcgcctaccg
      721 gatcatgctg cgcatcggcc gcctcgcgga gcctgagagg tggttcgggt gagtgccgtg
      781 accgacctcg cgctcgccgt gctgctgggc ggcgccttcg cgctgggcgt gctcggcgtg
      841 ctgtcggcgc tgccacgctg gggtgccctg ccactcgatc gccgcatcgc gccgtacctg
      901 cgtgacgtgg ttcccgacga ggcgatgccc tctggcatcc tgccgcgcgt cgggatgctg
      961 ccggtgcacg gccgcacggt gtgggagcgt gtgaagtcgg cattcgagcg gatgctcggt
     1021 ggcggggacg cgctggcgct ccgcctcgcg caggccggtt ccccgctcag ccccgcggtc
     1081 ttccgcggac gtcagctggc gtggggagtg accgggctcg tcgccggcgc cctgctggtg
     1141 gtgctgatcg ccgtcgccgg ccgcatgacg ccacccgctg tgttgctgcc gctcctctcc
     1201 gcggccggag tcgcggtcgc gtacgacatg cagctcaccg ggcgagcgaa atcacgtcgg
     1261 gcgcggctgg cggacgaact gcccaccacc ctggagttcc tcgcactgtg cctctcggca
     1321 ggggagtcgc tgctggatgc actgcggcga gtgtccggga tcggcaccgg cgagctcacc
     1381 gaggagctgc ggcatgccgt gctggcggtg cacaccggtt caccactggc cgacgctctc
     1441 ggcgagaccg ccacccggct gcagctgccc ggactgacgc gcgcggtcga ccagatcatc
     1501 gcggcgctcg agcacggggc gccgttggcc gccgtgctgc actcccaggc tgcggatgcg
     1561 agggatgagg ccaagcgcgt gctcatcgag caggccgggc acaaggagat cctgatgctc
     1621 ctgccgctgg tcttcctgat cctgccgctg agcgtgctct tcgccgtcta ccccggcctc
     1681 ttcatcctcc ggctcggcct cggctgagcc acaccaccac atgcaccgac cgacacgaga
     1741 cagacacgaa cgaggagcag acatgacgac gctcaggaga tggttcaccg caggcaggac
     1801 gctcgccgag gacgagaccg gggatgtgcc cggctgggtt ctggttacct tcgtgactgg
     1861 tgccagatac cgtgcctcac ctatgtgaag cgggggcgac ggcgttctct gactctccga
     1921 gctgtgatcg gatctgcagc catcccctca agcgatctcg ccgcccgcat cagcagctga
     1981 tagaaccgag ccggcgggat gcccagctca gtcatgatct tggcttcctt cccgggcgga
     2041 tggccggggt gtcgtcgctc gaacgcgagc aggtcagatg gcgtcacggt ctctaccctt
     2101 ccgccgaccg ccgacatacg ctgtcgtgat ggaggatctc gactggggtg cgcagccgcg
     2161 atgcccggag gacggcatcc tcatgcgcga cgtgccggga gggtgggagt gcccgcactg
     2221 tggtcacgtc cagcaggctc aggatgtgga gccgccgccg gagttcgacg ggccgtcgat
     2281 ctggggcggc tagtggtcac ccttgccaag tgagcgtccg aaaacgaatc cgagcgcagt
     2341 ggtggccacg agcttcaaca tgtcgatgat cccgctgagg ttcgcgccag atgctgggtc
     2401 gacgtagaag tcgatgaccg ccagtgtcac ggccagcacg ccacaacttc ccaccagcca
     2461 gaatgcgaat ttgagcttgt cccggtccat cccacgacga tgatcccctt cggggtcacg
     2521 cggcgcgggc accaatgcgc cgttacctcc tgggggaacg tcctcagtcg catcgtccgg
     2581 gacagacgac tcggtcacct ctcgacaagt ccgagtggtt ctaggtatgt cttgtcgccc
     2641 aggacggccg catcgctgag atacccgtaa ctgccactgt gctgactcca cgcagagcca
     2701 gccaggtgtg tgatgcgcga gagctcgacg gcagacacag gcttggtgac tcgccacacg
     2761 tcgctcagcg cagcgctgag cgcagcatcc ttggattcgt cggccatgta tgccttgccc
     2821 tgcgcatcct ttccgaatgc ccgaatctca ccgccaccga acgaacggaa ctcgtcgtag
     2881 acggtgcgca cgacggggcc gtaacgccaa ggctggaagc cttcgctcag caacggcctg
     2941 cctgtgcgct tggcgtactc gctcgccaca aaatagagga tcttctgcag cttcatcgga
     3001 gagacactga ccttctcctc gaacgcgcgc ttcaagatgt tgttgctgac attgacggca
     3061 ctgaaaacca tgtttctcca ccacccctct ggttgacact ctacggtaac gcgatcggcg
     3121 tgtcgtgtat ttcggatcgt agaagaggct actgacatca gcagtcggag gcctcgatca
     3181 atggagggtg acctgacggt ggtcgcgcac gaacgcctcg tgatgcgtga acatggcggt
     3241 ctcgtcccct cgatacgccc agatgatctg gtcgccgagc agtccgtgga gggtgatgat
     3301 cgcgccgtct tcgagtcgca tccatgtgtc gtggattcgg gccatgtcgc gacggtagcg
     3361 agaacgctct gcaacattgt tgcgagttca gtcgtaggcg cggtgcacgt gccagccgtc
     3421 ctcgccacgg tagacatcga agacgagcga taggccgtcg tcgtcggtgc cttggaagcg
     3481 ccagccgtag aggccgtggt gcggctcatc gaggggcacg gtccagatcg agtggcggat
     3541 ccgtgttggg gtgtcggtga cgcgccaccg tcggcccgcg tagaccatcc gtgcggggat
     3601 gtcgttgacc atccatatgg ttacagtgtg ttcgatgttg atggccgtca tgggttaaca
     3661 caatagaacg cgtgttcgaa gattgctacc ctgatggtgg ccggaggaag gggacgcggg
     3721 ggcggtggga acgaacaagc gctacgcgga ctcgatcgac cgcagagcga acgagagaca
     3781 catcctcgac ctctcgcgca cggcgcccct gcagtcgctc acggagaagg aactgcagct
     3841 cgacatctac ccgctcacga cgtatccgag gccgcagcgc tgccgcgtgt gggtgcggtt
     3901 cggcccgcac ccgttccggg tcgaggggcg cgtgcttcga accacgcagc tcgccgccgg
     3961 cgtcgagttc aagctcgagg agaccacgtt caagtgctgg gtgtggggcc ccgcgtgcga
     4021 ggtcatcacc ctcgacgacg tgatcgctga ccggaaggcg cacggcggtc tcgcctgagc
     4081 gtcggcgggg cggagtaggg ttcctgccat gtgcggacga ttcgcgatgg acgccaagac
     4141 cgacgagctc atccagcagt tcgtagccga cggcgggaag cctgaggact ggtggaagga
     4201 cttccacggg tcgtactcga tcgcacccac cgacgatgcg gtgatcgtcc gcgaccgcgg
     4261 cgagggtcgc agcctcgaac tcgtgcggtg ggactggcag aagcccgcca accgccccaa
     4321 gggcgccccg atcatcaacg cgaggatcga gaagctggac acagggttct ggctgccagc
     4381 gttcaccgcc gcacggtgtg tggtcccgat gcgtggctac ttcgagtgga ccggcgagaa
     4441 gggcgacaag accccgcatt tcctccatgg cgatggcctg ctcgccgccg ccgggctcac
     4501 gtggtcgatg gacctgccga gcggtgagcg gtcccgctgc atggtcgtcg tcacccggga
     4561 agccagggat gccggcggcg aggtccacga ccggatgccc gcgttcctca ccgaagacgc
     4621 catggccgcg tggctggaac cggtcaagct cgaccgcggc gaccgcgacg ggatgctgtc
     4681 cctcctcgac gcggcatccg ccgacgtcgc ctccacgatc cgccagtacg tggtcgaccg
     4741 gaaggtgaac aactcccgca cagtggacac aggcgaccag tccgtgatcg cccccgcggc
     4801 ctaagctgag agccatgctc accggtcaga tccgccccgt caaaacccgc atcctcgacg
     4861 tcgaggggac gcacgacgag atcgtggccg cactgaacgc cgcggccgtc gatgggtggg
     4921 aggtcgactc catcacgacg cggcgactcg cccactatgg cgcgacgacc accgtcgagg
     4981 cggcggacat cgacgccgtc cgggcgaagg tgcccgacgg acaccagctg ctggcggtga
     5041 cctcgtccgg actctgatgt cggaccatgc tcttatgatg aagacctcgc tgtaggggcg
     5101 aggctcacga ctcataggag gtccatcatg aagaagctca gcactatcgc ccaggcgctg
     5161 gctgaggaga tcgccgcgca ggactggtca gacgcgcata accgcgcaga cggtgctcgc
     5221 cacgaccgcg ccgctgatca caccacgacc acacagctgt ccccgacaga agccgagttc
     5281 gtgagggtga acgtgatgtg ggtgaccgcg caggcactgt ctgcccacga agcgaacttc
     5341 gacgtccacg cgttcgccga ggctgcaggc gtagacccgc gattccgcct tcgatcggac
     5401 ggcagcccaa gtggcgccat cccggccgga ctgcgttgga gcgaccagac gtacaagcct
     5461 gcggacctcg cacggctcct cggctaccgc gacgaggcgc gccccgggaa ggtggtccgt
     5521 gactacctgc ggaagaccta tcccgatcac cagaagcaca agccatggct cctgtccgaa
     5581 gaagaggcag ccgacgtgct cgcgaacgtc ccgcggaagc gctgaccact cacgcaacga
     5641 cgaaaccgcc ccaccctccc gaaggagagt ggggcggttt gagtgtgcgg acgctactcg
     5701 ccggaggtga ctgcgatcgc gttctgcatc gtggtgttct gctcgtcggg gtccagcttc
     5761 gcgaagtctg cgaggacgcg gtcacgaacg gcaggatcac gccacgtgcc gtcctcgtcc
     5821 tgcgacagca cgacatcacg tgaggtgcgc agttcgagtg cggtggcgga gctcttggtg
     5881 accatggtgt aggaggcctg agcgacgacg atcgcgagca gcgcgagcgc cggccactcc
     5941 gggacagtgt cgcccgtgta catgtagtag aagcccagca cgacaccggt gagggcgagc
     6001 gcgaccgcga cggccgcgac cttcttcaca atcggcgacc acgaggggcg ctgaatgagc
     6061 gcctgcacgt acggggcgag cagtgccagc agcacgagca cgcctgcggg gatggtgggc
     6121 agaatgatgt ccatcacaga ttcctttcag agcgggacgg gtagtccggc agggtgccgg
     6181 gcccgaggca gggggcgtcg atgatgagtc gacggtgcac ggcgagggac tcctcgacga
     6241 tgcgtgtgcg gcgcgcctgc gcctcttccg cggcttccgc ggcttccgcg cgggcgagtg
     6301 ccttgtcctt cgcggtctca gcgcggtcga cctctgcgcg gcgctgctgc agctgaccgg
     6361 cgcgcgcgtc cttgatgagg tcgaagagct tgagcacgat cccggagacg acgctggacg
     6421 acaggatgat cgtgatcagc tgggcggcct ccatggtcac ccccgacgtg gttcgaagct
     6481 gtagtggcgg atcagcatcc accgcactgc gaacacggac atggccagca ggatcatccc
     6541 aagctgcgtg agccgggaac cctcctgcgt gaggtgcagg gacaggacga ccagcccgta
     6601 gatcgtgatg cccgtcatgg tgaggatcag cgacaggcgt tcagcccacc accagcccgg
     6661 gagcacggtc agagcgccgg ccgtgccgcc gacgatcagg aacacggacc agatgaccgt
     6721 gagcaccgac ccgagctcac cctcgatcga ccgcggcggg ccgagcaggg tcgcgatccc
     6781 ggtggccagc acgagggcgt agatggccgc gtacgtgacc ttcatgtgcc gaggctcggt
     6841 gatcgactcc cagagcctgc gaagcggggc catcaccacc acccccagga cggcgcgcac
     6901 atgagcgtga gcgccgcgag gccgacggcc aggagcacgc cgagcccgat ccactcccgg
     6961 agggggatcg ggtgtcggcg cgcctggccg tgctggatgt tggtgccgcg ggtcatgtcc
     7021 acggcagccg gtacttggcg atcgcccaca ccaggtactt cgcggtcgcg aagccggcga
     7081 tgccgtcctg cgtcagcgtg ccgtacccgt tggacttcgc ggcgccctgc tcatgccgca
     7141 ccgcggcctg ggtcttggcg ccgtcggccc cgtcgacggt gagggggtgg ccgagctcga
     7201 ccagcatccg ctggatcagc ttcacccacg cctgcccgta ccgggtccgc gccggctggc
     7261 cggcggtctt ctcgcccttc gttcggaact tctggaccgc ccacagcagg tacgtcagcg
     7321 acgcgggccc ggcgatcccg tcctgagtga gggtgccgta gccgttcgac ttcgcggcgc
     7381 cctgctcgtg cttgacggcc gcctgcgtct tggtgccgtc cttgccgtcg cggccaccgg
     7441 tgtcgtggcc gaggatcgcg agcagatcct gcacgaacgt gacccagccg ccgccgtaga
     7501 gcttgcgggc ggggtagctg ccggatgcgc cgccggcggg tgccggtgcg gcgggcgtcg
     7561 ccgtggtgac gttgtagccc atcacccagt tcgtccagca caggaacttc cggccccagg
     7621 ccttctcgat cgccgagatg ctggtcacgc cgacccggcc ggacgagggg atgtcggtgg
     7681 agacgacgtt cccgccgccg acgctgatgc agatgtggcc gtacccgttg cggccggcgg
     7741 agaagaacac gatcgcgccc ttcgggggcg tgcggtcgta gtgccggttc gccgcgggga
     7801 cggtctcgag cgcacgcagc gcgtacgtgt agaagccggg ccggtcggag agcggcttct
     7861 gcagtgcgcg gtcgaccgcc tgcaggcaca ggccgctccc gtaggttcgc cacgacatca
     7921 tccggtcggc gccggcgatg ccgctcacag tgcccttggt cacagctcct cctcggtctg
     7981 cgtctcggct tcgggaacat ccgcgatgtc ctcgaacacc tcgatctcgg tgtcgttcac
     8041 ttcgggcgtg ctcatgacgc cctcctttca tcgggggtac gagaaagccc cgcgcgatgg
     8101 cggagctcag ggtggagtca ggcttccggt tccggggcgg gccggagcgc ggtgatcgcg
     8161 acggccaggt gcgtgtcggt gaccgcgccg aggttctcgc cggggcgagg cggcgttgcg
     8221 gcgacgtact gttcacgtgc ggtggcggcg taggcgtgaa cgtcggagac ggtctgcgtg
     8281 ccgtcgatcg tgatgcagac cagccggccc atgttcgcct gcacccatgc ggatgcgtcc
     8341 ggcacgtcgg cctgttcggc ggcggcgacg aaccgggcga gcaggtccgg gtcggtgcgg
     8401 gcgttgatgt gctgtgtggt ggtggccacg atgtcccctc tcagaaggct ttgatgatgt
     8461 ggttggtgac gacgtacggc tgcaggttgt tgtgcacgcc gtcgccgccg gcgttggcgg
     8521 tccccaatgg ctgtcccccg ccagagcccc cactggtttc tgcgatccgc tgccgccata
     8581 cgccgtcctc accggaggtg ccggcccagt tgaggttggt ggcgcggcgc atgccacccg
     8641 tcggggactc cacgtagaag aagccggatg gtgctccgtg gttgtgcgtc ggcatctccg
     8701 acggcgtcag cttgtgcgcc ttctcgccgc cggtctcgcc gagcacgtcg aactcggtct
     8761 gcccgctgtc gcggccgacg gggatgcgcc ctcgcaggtc gggcacgttg aacgtggacg
     8821 acccgttgcc cgcaccgtag acggtgccga tcacggcgaa cagcgcggcg taagtggtgc
     8881 ggctgacggc cgcgccgtcg cacagcagcc acccgcccgg ggcggtcgcg ccggcgaacg
     8941 cgacgaggat gccgaccggt tcgacggcgt acccgccgcg gtgccgcagc tccaagtccg
     9001 cttcgaggtc gccgtcgtac acgcgcgccg ggccctgcaa ctgcagggct ggcccggcat
     9061 cctcgaccat cttcccgatc gcgaccttcg tcggcgtcgc gtcgaggatc gcgcccgcgg
     9121 tcggcgcaag gtagaagtcc tgcgcggact gcagcttgtc ggccaccgtc acgcggacgt
     9181 cccacgcggt ggtcgtcatg aagatgccgc cgccggacac cagcaccgac gtgttgtagg
     9241 tcaggccggg ggtgatcacg ttccggtcgg tccacgccga cccgccgcgc gggcgggtgg
     9301 cgacggtgat ggtcgcggcg ttcttctccg acccgaccac caggctcgag acggccgcgt
     9361 tcagcgtcat caggaggttg gtgccggtgg aggaggggac gccggcggcg gtcgcgcgct
     9421 gcacgctgaa gtcgatgatc tgtggcaggt cgtacgggag gacggtgacg gtgccggagt
     9481 cggccgcggt gcggccgcgg gagtccgtga ccgccgcggc gaccactcgg ctgccggact
     9541 gcgggagcgg gagggactga ccggaggtga cggtctgccc gtcgaccgtg accatgcggc
     9601 tgctgatcgt ggacccgtac gcgcccgccg cgatcacatc ggccttgagc cgggactggc
     9661 cctgcacgag tccgccgatc aggttggcga cgtcggggtt gtcgtccgag acgttgatgc
     9721 cgctgatcgt cggcaccacc gtggccgggg cggtgagggt gaagttcttc tgcttcgtcc
     9781 cgatgacggt ggagccgttc ttggtgacga ccgtgaccgt gcccgtgccc gaggtgttgt
     9841 tcgggatctc cgtcaggagc gacatcggtg gcgtccacgc ggcggacacg cccgcgccgg
     9901 tcgcgatcgt ccccgactgg ttgccgaact ggaagctcac gtcgtgcgtg aagctgctcg
     9961 acgcgcggtc gaggaagacg gtgacggcgg cgccggcgtc gaacgtggtg acgtcggtcg
    10021 acccgactcg gaaccgcggc gtcgtggcgc gcgggatggt cggaagagcc atcgaatagg
    10081 cgtcgccgtg gttgatgttt ccgtacgcca cggacatgcg gatcacggct ggggagcgcg
    10141 tgccgtccgg gttgtgcggg atgtagaccc gcttgtagat gtcccagcgg gtgacgttgc
    10201 tgttgacctt gttcaggaag ttcgaggaca cctgccagcg agcgaactcg ccgacgccgt
    10261 cgatcgagcc gacgtgcgta ccggcaccgc cgtaccacga cgtcgtgccg ctaccggtgt
    10321 tgatgttccg gatgcggacg tcgacgatcg agtagttccc gtcgatcgac atgccgatgt
    10381 aatcggcgtc gagcaggaac tgggtgatgg gccccgtctc gttgaatgtc agcctgaccg
    10441 tcaccgtggg cccccgttca tgcaggaagg gccaccccgc gtggagtggc ccttccgtgt
    10501 gtgatcagag gcgctgccag gtcgtgcgac cggcgccctc cgtgatgacc gtctgaccga
    10561 cgaccacctg acttgccttc aacttcggga cgatcatctg gttctcgtcc caccaggtct
    10621 gcgcgacacc gttccgccgg aagctcgcct gatcgttccc caggtggaga gcgaccggag
    10681 cgcccggacg ggagatccgc acatcctgcg gtgtgatctg caccgccagc tgcagcgcgt
    10741 ccacaccgga ctgcgccgcc gcggcctgag cctgcgcccc gaaaagaccg tgctgatgcc
    10801 g
//
LOCUS       DATMSX010000192         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_107277,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000192 DATMSX010000000
VERSION     DATMSX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107277"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VN041_05965"
     CDS             complement(<1..888)
                     /locus_tag="VN041_05965"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HWU28626.1"
                     /translation="MKAVHFGAGNIGRGFVGLLLHQGGYELVFSDVAAALVDAINAAD
                     EYTVHEVGDGGADTVVTGFRAINSAEDPEGLIAEIADADVVTTAVGPTVLRFVAAPIL
                     DALRRRDAQAAPLQVMACENAIGATDLLRQEIIARAGEDWGALAGRAVFANTAVDRIV
                     PGQPEGAGIDVTVEPFFEWAIERGPFGENPPRIPGAHFVDDLAPYIERKLFTVNTGHA
                     ATAYLGAQAGYERIADALADDAISTAVAAALEETSALLAVKHGLDPAELAEYRATILR
                     RFANPALPDTVWRVGRQPLR"
     gene            complement(885..2894)
                     /locus_tag="VN041_05970"
     CDS             complement(885..2894)
                     /locus_tag="VN041_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWU28627.1"
                     /translation="MTTTSPATRGGIRVGVQRFGTFLSGMIMPNIPALIAWGIITAFF
                     IPAGWTPNAQLAELVGPMIHYLLPIVIAYTGGTLVYKVRGGVVAAIAVMGAIAGSDYL
                     IAQVNAADLQAWVDAGNTADTFRALGQVHMFIGAMIMGPLAAYTMKWLDGLWEGRVKA
                     GFEMLVNMFSAGIWGFVMAVVGFYPVAWLVNGIMNVLSAAVNWLVATNLLPLTSIIIE
                     PAKVFFLNNAINHGVLTPLGTQQATDSGKSILFLLEANPGPGIGLLLAFALFGLGAAK
                     ASAPGAAVIQFFGGIHEVYFPFALMKPVLILALIAGGMTGVTTNMLFHTGLIAPAAPG
                     SIIAVLGQTYRTDYVGVILSVVLSAAVTFLIAGLILRASRRKDLAKEDAFAAAVAQTE
                     ANKGKKSSVLGSLAGAGTGVTTRPIASIVFACDAGMGSSAMGASVLRNKIKKAGIPGV
                     TVTNAAIANLDGTADLVITQQQLTDRAKAQSPASEHLSVDNFMNSPVYDDVVTLLQNQ
                     GAAPAASEEQVAAEAPSSAVLTPDRVRIHSGSATRDEALKEAADALVTAGAVTPDYLQ
                     AMHDREKSVSTFMGNGLAIPHGTNEAKDAVLGSALSVVRYDGGVDWDGDRATFVIGIA
                     GKDGAHLQILSSIAELFSDDDDVAALNAAATPEELFALLSAVNEA"
BASE COUNT          438 a          999 c         1091 g          460 t
ORIGIN      
        1 ccgcagcggc tgccggccca cccgccagac ggtgtcgggc agcgccgggt tggcgaagcg
       61 gcgcaggatc gtcgcgcgat actcggcgag ctcggcggga tccaggccgt gcttgacagc
      121 cagcagcgcc gaggtctctt cgagcgcggc ggccaccgcc gtgctgatcg cgtcgtcggc
      181 cagggcgtcg gcgatgcgct cgtaacccgc ttgcgcgccg aggtacgcgg tcgcggcgtg
      241 ccctgtgttc acggtgaaca gcttgcgctc gatgtagggt gcgaggtcgt cgacgaagtg
      301 cgctccgggg atgcggggcg gattctcgcc gaacgggccg cgttcgatcg cccactcgaa
      361 gaagggctcc acggtcacgt cgatcccggc gccctccggc tgcccgggca cgatgcggtc
      421 gacggcggtg ttcgcgaaca ccgcccggcc ggcgagggcc ccccagtcct cgcctgcacg
      481 cgcgatgatc tcctgcctca gcaggtcggt ggcgccgatc gcgttctcgc acgccatcac
      541 ctgcaaggga gccgcctgcg cgtcccggcg ccgcagggcg tcgaggatgg gcgcggcgac
      601 gaaccgcagc acggtgggcc cgaccgcggt ggtcacgaca tcggcatccg cgatctcggc
      661 gatcagaccc tccgggtcct cggcgctgtt gatcgcacgg aagccggtga ccacggtgtc
      721 ggcgccgccg tcgcccacct cgtgcaccgt gtactcgtcg gccgcgttga tcgcgtcgac
      781 cagggcggcg gcgacgtcgg agaacaccag ctcgtacccg ccctggtgca gcagcaggcc
      841 tacgaagccc cggccgatgt tgccggcgcc gaagtggacg gccttcatgc ctcgttcacc
      901 gccgacagca gtgcgaagag ctcctcgggc gtcgccgcgg cgttgagcgc ggccacgtcg
      961 tcgtcgtcgg agaacagctc ggcgatgctg gagaggatct gcaggtgcgc accgtccttg
     1021 cccgcgatgc cgatcacgaa cgtggcgcgg tcgccgtccc agtccacgcc gccgtcgtag
     1081 cggaccacgc tgagcgccga gccgagcacg gcgtccttcg cctcgttggt gccgtgcggg
     1141 atcgccagcc cgttgcccat gaaggtcgac accgacttct cgcggtcgtg catggcctgc
     1201 aggtagtcgg gagtgacggc acccgcggtc accagcgcgt ccgcggcctc cttcagcgcc
     1261 tcgtcgcggg tcgcggatcc ggaatggatg cgcacgcggt cgggggtaag gacggcggag
     1321 gacggcgcct cggccgcgac ctgctcctcg gatgccgcgg gtgcggcgcc ctggttctgc
     1381 agcagggtca cgacgtcgtc gtacaccggg gagttcatga agttgtccac ggacaggtgc
     1441 tcggatgccg gggactgcgc cttcgcgcga tccgtgagct gctgctgcgt gatgaccagg
     1501 tcggcggtgc cgtcgaggtt cgcgatcgcc gcgttggtga ccgtgacgcc ggggatgccc
     1561 gccttcttga tcttgttgcg cagcacgctc gcgcccatcg ccgacgagcc catgcccgcg
     1621 tcgcaggcga acacgatgct cgcgatgggg cgggtcgtca ccccggtgcc cgcaccggcg
     1681 agcgagccca ggacgctcga cttcttgccc ttgttggcct cggtctgcgc gacggcggcc
     1741 gcgaaggcgt cctccttggc gaggtccttc ctgcggctgg cccgcaggat caggccggcg
     1801 atcaggaagg tcacggcggc cgagaggacg acggacagga tcacgccgac ataatcggtg
     1861 cgataggtct ggccgagcac cgcgatgatg cttcccggcg ccgcgggggc gatgagcccg
     1921 gtgtggaaca gcatgttcgt ggtgacgccg gtcattccgc cggcgatcag ggccaggatc
     1981 agcacgggct tcatcagggc gaacgggaag tacacctcgt ggatgccgcc gaagaactgg
     2041 atgacggcgg cgccgggagc cgaggccttc gccgctccca ggccgaacag cgcgaacgcg
     2101 aggagcaggc cgatgccggg gccggggttg gcctcgagca ggaacaggat cgacttgccg
     2161 ctgtcggtcg cctgctgcgt gcccagcggc gtgagcacgc cgtggttgat cgcgttgttc
     2221 aggaagaaca ccttggcggg ctcgatgatg atgctcgtca gggggagcag gttggtggcg
     2281 acgagccagt tcacggccgc ggagagcacg ttcatgatgc cgttgaccag ccaggccacc
     2341 ggatagaagc cgacgaccgc catcacgaag ccccagatgc cggccgagaa catgttcacg
     2401 agcatctcga agccggcctt gacccggccc tcccacaggc cgtcgagcca cttcatggtg
     2461 taggcggcga gcgggcccat gatcatcgcg ccgatgaaca tgtgcacctg ccccagcgcc
     2521 cggaacgtgt cggcggtgtt gcccgcgtcc acccaggcct gcagatcggc ggcgttcacc
     2581 tgagcgatca ggtagtcgga tccggcgatg gcgcccatca cggcgatcgc cgcgaccact
     2641 ccgccgcgga ccttgtagac cagcgtgccg cccgtgtagg cgatgacgat gggcagcagg
     2701 tagtggatca tcgggccgac cagctcggcc agctgcgcgt tcggagtcca tccggcgggg
     2761 atgaagaagg cggtgatgat gccccacgcg atcagggcgg ggatgttcgg catgatcatg
     2821 ccggacagga acgtgccgaa tcgctgcacg cccacgcgga tgccgccacg ggtggcgggt
     2881 gacgtcgtcg tcatggtctt ctctttctga tgcggggtgg gggggaagtg gtcagtgctg
     2941 tgcggcgtcg agcgctgcgg cgcgggcgcc ggcggcgtcg tccgccag
//
LOCUS       DATMSX010000193         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_2987,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000193 DATMSX010000000
VERSION     DATMSX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1902)
                     /locus_tag="VN041_05975"
     CDS             complement(109..1902)
                     /locus_tag="VN041_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HWU28628.1"
                     /translation="MHRRPLRAVAALTAAVALAITGAPLAASAAPAASPPGHERAAAA
                     YAQTTMESMTLEQKIGQLFILFAYGPTATTDDPRNTALYGEADAADIISEYEPGGFIL
                     FSARDNVTDPVQVATLANGLQDAAQHAGAGIPLLVSTDQEQGIVTRIGSPATLFPGSM
                     ALGAGRSAKDTRKAAAITGQELAAMGIQQNNAPDADVNVNPDNPVIGVRSFSSDPALV
                     SELTAAAVQGYQDDAGIVATAKHFPGHGDTATDSHTGLPLITHTLEQWQQIDAPPFKA
                     AIAAGVDSIMTAHIVVPALDDSGDPATLSKKIVTGQLRDALGFQGVIVTDGLEMAAVR
                     QKYGDGEVAVRALEAGVDQLLLPAAPQEAYRAVEDAVATGRITEQRIDESVERILKMK
                     YDQGVTKHAKVDTGKVMNVVGKNSSLRAAEKITDRTTTLIRNENGALPVAVDGKKVLV
                     TGYGVVTGQTLAGELTARGADATALSTGTAPTQAQIDQAVAAAQTSDVTVVLTNGVTA
                     TSTQKALVAALDATDSHLVVVGVRNPYDINQLGDVDDYIATYSYSAPALASLAKVITG
                     EISPTGKLPVDIYSADDPTQILYPFGYGLTW"
     gene            complement(2033..2941)
                     /locus_tag="VN041_05980"
     CDS             complement(2033..2941)
                     /locus_tag="VN041_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWU28629.1"
                     /translation="MAETTGRVAVYLDFDNIVISWYDRVHGRNAYGKDRQKITDDPHD
                     AEVTERLRQAMIEVGAIIDYASSFGTLVLTRAYADWSSPVNAEYRSQLVARAVDLVQL
                     FPAAAYAKNGADIRLAVDAVEDMFRLPDLTHVVVVAGDSDYVPLAQRCKRLGRYVIGV
                     GVAGSTAKSLAAACDEFATYDSLPGVVRPKKDAAPAGQAKDAKAKADQKPATTRRAKA
                     TTKPDEQGEATLLLERALQLGHDKADADEWLHASAVKTQMRRMDPSFSEKALGFRSFN
                     DFLRSRDDIAELEESGHERLVRLRER"
     gene            complement(2944..3960)
                     /locus_tag="VN041_05985"
     CDS             complement(2944..3960)
                     /locus_tag="VN041_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010523714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU28630.1"
                     /translation="MTGLRWGILATGGIAASFASDLRTAGLDLQAVGSRSQESADAFA
                     ARFDIPRAHASYESLVADPDIDIVYVATPHPMHHENARLALEHGKHVLVEKAFTVTRA
                     QAEDLQRLAAEKGLIAMEAMWTRYLPHMVRIREIIADGTLGEIRSVMADHTQSLPTDP
                     AHRLNALELGGGALLDLGIYPVSFIWDVLGEPVTVQASARLVETGADAEVATIMTHTG
                     GAISTSFSSSRTAGPNTATVLGTKARIEIDRVWYSPTSFRVIGTDGTVLEDYASQVDG
                     RGMQYQAFAAEGAVAAADPAGGVLALPIAESVAIMGTLDEIRRQVGVVYPGVDDAHAD
                     GEAS"
     gene            complement(3989..5044)
                     /locus_tag="VN041_05990"
     CDS             complement(3989..5044)
                     /locus_tag="VN041_05990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU28631.1"
                     /translation="MAIDLGYWTPVYGGFLRNVRDEGRMAATWDYIREVSVKADRLGF
                     HTTLVPELYLNDRKGTDAPSLEAWSLSAAILAATDRLRVMTAVRPGFHLPAVLAKTVS
                     TLDSIAPGRVALNVVAAWWAEEARQFGGVFPAHDARYAQATEFVEVLNGLWESTPFSY
                     RGDHYEFDGTIVEPKPSRHPVVFAGGESDAGREAIAGFADAYVMHGGTLEEVRTNVSD
                     MNARSERLHGRPIAEFGMPAYVIVRDTEAEAQRELDRITTVDPHSPGYASFEQFRQNS
                     NLSLELSRREYSVGTRGLRPNLVGTAEQVAERIGEYADAGISLLLIQASPLDEELDRI
                     AEQVFPLVKQRSLVTSV"
     gene            complement(5083..6375)
                     /locus_tag="VN041_05995"
     CDS             complement(5083..6375)
                     /locus_tag="VN041_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HWU28632.1"
                     /translation="MTEGFATTQVQGGYAVGLPSFSAVPPIHQTAAYEFSSLQEAADL
                     FALRKAGIIYSRNASPTQQILEERVAALEGGASAVAVASGQAAVAVTLLALAGRGGHI
                     VAADQLYGGTVDLLQDTFDDFGIPVTFVDQDDPDAWRAAVTPQTRALFAETVANPVAQ
                     VLDIRLIADIAHEAGVPLVVDSTVGTPALIRPGDHGADFVVHSATKFLSGHGSSLGGV
                     VVDQGTFDFGAEPERWPQFTAPYQRFGELVLWERFGQGQAFAALVKSKYVHDLGPSLS
                     PFNAWQILQGIETLDLRVARQSSSALAIAQHLAQHPAVSRVNHPGLADSPWHALAQRY
                     LPKGVPSVFSFDVDGTEDEAPARIARLVDELNVFRLVANLGDARSLVAHPASMTHSHL
                     TSAQRAAAGISLNTVRLSIGIEDPADLIADLDRALAIA"
     gene            complement(6372..>6576)
                     /locus_tag="VN041_06000"
     CDS             complement(6372..>6576)
                     /locus_tag="VN041_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28633.1"
                     /translation="LAAAALVSIPDLEVVETVGGLHDALNDASHRSVAASIVLWLERL
                     RAGDVHEPVIRRAVPAHEQGAAA"
BASE COUNT          922 a         2224 c         2380 g         1050 t
ORIGIN      
        1 gcgctgctgc tgacggccgc gccggcggcg gtggccgcgg cgactcccgc tgcgccgaag
       61 agtcctcgtc tgctgatgtt catgagtctt ctcctcctcg tccttcggtc accacgtgag
      121 cccgtagccg aacgggtaga ggatctgcgt cgggtcgtcg gccgagtaga tgtcgacggg
      181 cagcttcccg gtggggctga tctcgccggt tatgaccttc gcgagcgacg ccagcgcggg
      241 agccgagtac gagtacgtgg cgatgtagtc gtcgacgtct cccagctggt tgatgtcgta
      301 cgggttgcgc acgccgacca cgaccaggtg gctgtccgtg gcatccaggg ccgcgaccag
      361 cgccttctgt gtgctggtgg ccgtcacgcc gttggtgagc acgaccgtga cgtcgctcgt
      421 ctgcgcggct gcgacggcct ggtcgatctg agcctgtgtg ggcgcggtgc ccgtcgagag
      481 cgcggtggca tccgcgccgc gtgcggtgag ctctccggcc agcgtctgtc cggtcaccac
      541 gccgtagccg gtgaccagca ccttcttgcc gtcgacggcg acgggcagag cgccgttctc
      601 gttgcggatc agcgtggtcg tgcggtcggt gatcttctcc gccgcgcgca ggctggagtt
      661 cttgcccacg acgttcatca ccttgccggt gtcgaccttc gcgtgcttcg tcacgccctg
      721 gtcgtacttc atcttcagga tgcgctcgac cgactcgtcg atccgctgct ccgtgatgcg
      781 gccggtggcg acggcgtcct cgaccgcgcg gtaggcctcc tgcggggcgg cggggagcag
      841 cagctggtcg accccggcct cgagtgcgcg cacggccacc tcgccgtcgc cgtacttctg
      901 acgcaccgcg gccatctcca ggccgtcggt gacgatcacg ccctggaatc cgagcgcgtc
      961 gcgcagctgg ccggtgacga tcttcttgga cagcgtggcg gggtcaccgc tgtcgtcgag
     1021 cgccggcacc acgatgtgcg cggtcatgat cgagtccaca cccgcggcga tcgccgcctt
     1081 gaagggcggg gcgtcgatct gctgccactg ctcgagcgtg tgcgtgatca ggggcaggcc
     1141 ggtgtgcgaa tcggtggcgg tgtcgccgtg gccggggaag tgcttggccg tcgcgacgat
     1201 gccggcgtcg tcctgatatc cctgcacggc agcggcagtg agctcggaga cgagagcggg
     1261 gtcggatgag aagctgcgca cgccgatcac ggggttgtcg ggattgacgt tgacgtcggc
     1321 atccggggcg ttgttctgct ggatgcccat cgcggcaagc tcctggccgg tgatggcggc
     1381 tgccttgcgg gtgtccttcg cgctgcgtcc ggcgcccagc gccatgctgc cggggaagag
     1441 cgtcgcaggg gagccgatcc tggtgacgat gccctgctcc tgatccgtgc tcaccagcag
     1501 cgggatgcca gcgccggcgt gctgggcggc atcctgcagg ccgttcgcga gcgtggcgac
     1561 ctgcacggga tcggtcacgt tgtcgcgagc cgagaacagg atgaacccgc ccggctcgta
     1621 ctccgagatg atgtcggccg cgtccgcctc gccgtagagg gcggtgttgc gcgggtcgtc
     1681 cgtcgtggcg gtcgggccgt aggcgaacag gatgaacaac tgcccgatct tctgctccag
     1741 cgtcatcgac tccatggtcg tctgcgcata cgccgcggct gcacgctcgt ggccgggggg
     1801 cgacgcggcg ggggcagcgg atgccgccag cggggcgcct gtgatcgcca gggcgacggc
     1861 tgccgtcagg gctgcgaccg cgcggagggg ccttctgtgc atgacgttct cctcatcgag
     1921 attcgtcccc agctcgcccc cagacgtgcc cgtcagccta gcaacgcctc gcgctcttgg
     1981 acagcctctc gctcttcgac ggagcggcgg cgccccgggc gagcaccgtg cgtcaccgct
     2041 cgcgcaggcg caccagccgc tcgtgcccgc tctcctcgag ctccgcgatg tcatcgcgcg
     2101 aacggaggaa gtcgttgaac gagcggaagc cgagcgcctt ctcactgaac gaggggtcca
     2161 tgcggcgcat ctgggtcttc accgccgagg cgtgcagcca ctcatcggca tccgccttgt
     2221 catggccgag ctgcagagcg cgctccagca gcagcgtcgc ctcgccctgc tcgtcgggct
     2281 tcgtcgtagc cttcgcgcga cgggtggtcg ccggcttctg atctgccttc gccttggcat
     2341 ccttcgcctg gccggcaggc gcggcatcct tcttcggccg caccacgccg ggcagcgagt
     2401 cgtaggtcgc gaactcgtcg caggccgcgg ccagcgactt cgccgtggag cctgcgacgc
     2461 ccaccccgat cacatagcgg ccgagccgct tgcagcgctg cgcgagcggc acgtagtcgc
     2521 tgtcaccggc gacgacgacc acgtgcgtga ggtcgggcag acggaacatg tcctccacgg
     2581 cgtcgacggc caggcggatg tctgcgccgt tcttcgcgta ggccgcagcg gggaacagct
     2641 gcaccaggtc gaccgcgcgg gccaccagct gcgagcggta ctcggcgttg accggcgacg
     2701 accagtcggc gtaggcgcgc gtgaggacca gcgtgccgaa cgaggaggcg tagtcgatga
     2761 tcgcgccgac ctcgatcatc gcctgcctga gccgctcggt cacctcggcg tcatgcgggt
     2821 cgtcggtgat cttctggcgg tccttgccgt aggcgttgcg gccgtgcacc cggtcgtacc
     2881 aggagatcac gatgttgtcg aagtcgaggt agaccgcgac gcgtccggtc gtctccgcca
     2941 tgatcagctg gcctctccgt cggcgtgcgc gtcgtcgacg ccagggtaga cgaccccgac
     3001 ctgccggcgg atctcgtcga gcgtgcccat gatcgccacg gactcggcga tcggcagggc
     3061 gagcacgcct ccggccgggt ccgcagcggc cacggcgccc tcggcggcga aggcctggta
     3121 ctgcatcccg cggccatcga cctgggacgc gtaatcctcc agcacggtgc cgtcggtgcc
     3181 gatcacgcgg aacgacgtcg gcgagtacca gacccggtcg atctcgatcc gcgccttcgt
     3241 gccgagcacc gtcgcggtgt tcggtccggc cgtgcgggac gacgagaagc tggtcgagat
     3301 cgcgccgccg gtgtgcgtca tgatcgtcgc gacctcggca tccgctccgg tctcgaccag
     3361 gcgggcggat gcctggacgg tgaccggctc gcccagcacg tcccagatga acgagaccgg
     3421 gtagatgccg agatccagca gagcgccgcc gccgagctcg agcgcgttca ggcgatgcgc
     3481 cggatcggtc ggcaggctct gcgtgtggtc ggccatgacc gagcggatct cgccgagggt
     3541 gccgtcggcg atgatctcgc ggatgcgcac catgtgcggc agatagcgcg tccacatcgc
     3601 ctccatggcg atcaggccct tctccgcggc gaggcgctgc aggtcctcgg cctgtgcccg
     3661 cgtgacggtg aacgccttct cgacgagcac gtgcttgccg tgctccagcg cgagccgggc
     3721 gttctcgtgg tgcatgggat gcggggtcgc gacgtagacg atgtcgatgt ccgggtcggc
     3781 cacgagcgac tcgtaggagg cgtgcgcgcg cgggatgtcg aagcgtgcgg cgaacgcgtc
     3841 ggcggattcc tgcgatcggg agccgaccgc ctgcaggtcg agtcctgcag tgcgcaggtc
     3901 ggatgcgaag gatgcggcga tgccgcccgt ggcgaggatg ccccaacgaa gaccggtcat
     3961 gggatcagcg tatcgggcgc cgccgatctc agaccgaagt gaccagcgag cgctgcttca
     4021 ccagcgggaa gacctgctcg gcgatgcggt cgagctcctc gtccagcggg gacgcctgga
     4081 tcagcagcag gctgatgccg gcatccgcgt attcgccgat gcgctcggcg acctgctcgg
     4141 ccgtgccgac gagattcggg cgcaggcccc gggtgccgac cgagtactcg cgacgggaca
     4201 gctccagcga cagattcgag ttctgccgga actgctcgaa ggaggcgtag ccgggggagt
     4261 gcgggtcgac ggtggtgatc cggtccagct cgcgctgcgc ctcggcctcg gtgtcgcgca
     4321 cgatcacgta ggcgggcatc ccgaactcgg cgatcggacg gccgtgcagg cgctcggagc
     4381 gggcgttcat gtcggagacg ttcgtgcgca cctcctcgag cgtgccgccg tgcatcacgt
     4441 acgcgtccgc gaagcccgcg atcgcctccc ggccggcgtc gctctcgccg cccgcgaaga
     4501 ccaccgggtg acgggagggc ttgggttcga cgatcgtgcc gtcgaactcg tagtggtcgc
     4561 cgcggtagct gaacggcgtg ctctcccaca gtccattcag cacctcgacg aactcggtcg
     4621 cctgcgcgta gcgggcgtcg tgcgcgggga agactccgcc gaactgccgc gcctcctccg
     4681 cccaccaggc cgcgaccacg ttcagcgcca cccggcccgg cgcgatgctg tcgagcgtgg
     4741 acaccgtctt ggcgaggacg gcgggaaggt ggaacccggg gcgcaccgcc gtcatcaccc
     4801 gcaggcgatc ggtcgcggcg aggatcgccg cggacagcga ccacgcctcc aggctcgggg
     4861 catccgtgcc cttgcggtcg ttcaggtaca gctccgggac cagagtggtg tggaacccga
     4921 gccggtcggc cttcaccgac acctcccgga tgtagtccca cgtggcggcc atgcggccct
     4981 catcgcggac attgcggagg aacccgccgt agacgggcgt ccagtatccg aggtcgatgg
     5041 ccatgagggt cctcctggtg cttcgggttc agagtgcggc ggtcaggcga tcgcgagcgc
     5101 gcgatcgagg tcggcgatca ggtcggcggg atcctcgatc ccgatcgaca ggcgcacggt
     5161 gttcagggag atgcccgccg ccgcacgctg ggcggaggtc aggtgcgaat gcgtcatgga
     5221 tgcggggtgc gcgaccagag agcgggcgtc gccgaggttc gcgaccaggc ggaacacgtt
     5281 cagctcgtcc accagccggg cgatgcgcgc cggcgcctcg tcctcggtgc catccacgtc
     5341 gaaggagaac actgatggca cccccttggg gagatagcgc tgcgcgagag cgtgccacgg
     5401 gctgtctgcg aggccgggat ggttcacccg ggagacggcc ggatgctgcg ccaggtgctg
     5461 tgcgatcgcc agcgcgctcg acgactggcg ggcgacgcgc aggtcgagcg tctcgatgcc
     5521 ctgcaggatc tgccaggcgt tgaacggcga cagcgagggc ccgaggtcgt gcacgtactt
     5581 ggacttgacc agagccgcga aggcctgccc ctgcccgaag cgctcccaca gcaccagctc
     5641 tccgaaccgc tggtacgggg cggtgaactg cggccagcgt tccggctcgg cgccgaagtc
     5701 gaaggtgccc tgatcgacga ccaccccgcc cagcgaggaa ccgtgaccgg acaggaactt
     5761 cgtcgccgag tgcaccacga agtccgcgcc gtgatcgccc gggcggatca gcgcaggggt
     5821 tcccaccgtg ctgtccacga cgagcggcac gccggcctcg tgggcgatgt cggcgatcag
     5881 gcggatgtcg agcacctgcg cgaccggatt ggcgaccgtc tccgcgaaca gcgccctggt
     5941 ctgcggcgtg acggccgccc gccaggcatc cggatcgtcc tgatcgacga aggtgaccgg
     6001 gatgccgaaa tcgtcgaacg tgtcctgcag caggtcgacg gtgccgccgt agagctgatc
     6061 ggccgcgacg atgtgcccgc cgcgacccgc gagggcgagc agggtcacgg cgaccgccgc
     6121 ctgaccggat gcgacggcga ccgcactcgc accgccctcg agtgcggcga cccgctcctc
     6181 gaggatctgc tgggtggggc tggcgttgcg gctgtagatg atgcccgcct tgcgcagcgc
     6241 gaacagatcg gcggcctcct gcagagagga gaactcgtag gccgcggtct gatggatggg
     6301 cggcacggcg ctgaaggagg ggagaccgac ggcgtagccg ccctgcacct gggtcgtggc
     6361 gaatccctcg gtcacgctgc cgccccctgt tcgtgagcgg ggacggcgcg gcggatgacc
     6421 ggctcgtgca cgtcgccggc gcggaggcgc tccagccaca gcacgatgct cgcggccacg
     6481 ctgcggtggc tggcgtcgtt cagcgcgtcg tgcaggccgc ccacggtctc gacgacctcg
     6541 aggtccggga tcgacacgag cgctgccgcg gccaga
//
LOCUS       DATMSX010000194         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_163687,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000194 DATMSX010000000
VERSION     DATMSX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163687"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="VN041_06005"
     CDS             <1..1824
                     /locus_tag="VN041_06005"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HWU28634.1"
                     /translation="LAVTETTFRDAHQSLLATRVRTSDLVAVAPYVARMTPQLLSVEA
                     WGGATYDVALRFLGEDPWERLAALRTALPNINIQMLLRGRNTVGYTPYPTEVTDAFVR
                     EAAATGVDIFRIFDALNDVSQMRPAIDAVLGTGTAVAEAALCYTGDLLNPAEDLYTLD
                     YYLRLAEQIVDAGAHVIGIKDMAGLLRPAAAAKLVTALRERFDQPVHVHTHDTAGGQL
                     ATLLAASGAGADAVDVASAPMSGTTSQPSLSALIAALAHTERDTGIDLQAASDLEPYW
                     EAMRHLYRPFESGLPSPTGRVYRHEIPGGQLSNLRQQAIALGLADDFELIEDMYAAAN
                     DILGRVPKVTPSSKVVGDLALHLAAVKADPADFAANPERYDIPDSVVGFMAGELGDLP
                     GGWPEPFRSKVLAGRAAKAGITPITDVQQAALDGTSAERRGMLNELLFPAPTKIFRQN
                     REEFGDLSVLDTSDYLYGLVPGVEHAVEIERGVRLYVGLEAIGEADPKGVRTVMTTLN
                     GQLRPVSVRDRSIVVDAHETEKADTSKPGQIAAPFSGAVTLKVEEGAVVKAGDAVASI
                     EAMKMEAAITTSVDGVVERLAIDQTQQVDAGDLLVVVRPAH"
     gene            1870..>2777
                     /locus_tag="VN041_06010"
     CDS             1870..>2777
                     /locus_tag="VN041_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28635.1"
                     /translation="MTSKNNVEPEGDDSLGVLDDTTSSDTIAIGLHEGTAQVSVALPT
                     GGEDDDLVDDEVIGGEVVSDLIIDTSAVADPDVLARISQDAPEIVIELPAEELPAAAE
                     TAEDRPVDPETVTELVVEAEEPGIEAADAVAHADDIVDADVVPESGGADAEVITAGDD
                     EIVADASDEAEADAGVETMTEAEAIAGETAAPPAPKPGIRPEPVLLPPLTAARGRKAV
                     RKPAKSKKKAEQPKRESPVDETTPAAAETAPAVENVTVATPEQVAKPEAAAKPEATAK
                     PEADAKSDQPVPPAKSEPQADETGSEP"
BASE COUNT          452 a          978 c          986 g          361 t
ORIGIN      
        1 ctcgccgtca ccgagacgac gttccgcgac gcgcaccagt cgctgctggc cacgcgcgtg
       61 cgcaccagcg atctcgtcgc ggtcgcgccg tacgtcgccc gcatgacccc gcagctgctc
      121 tcggtggagg cctggggcgg cgcgacctat gacgtcgcgc tgcgcttcct cggcgaggac
      181 ccctgggagc ggctggcggc tctgcgcacg gcgctgccga acatcaacat ccagatgctc
      241 ctgcgcggac gcaacaccgt cgggtacacg ccgtacccga ccgaggtgac cgacgcgttc
      301 gtgcgcgagg ccgccgcgac aggcgtggac atcttccgca tcttcgacgc cctcaacgac
      361 gtgtcccaga tgcgccccgc gatcgatgcg gtgctgggca ccggcaccgc cgtcgcggag
      421 gcggcgctct gctacacggg tgacctgctc aatcccgccg aggacctgta cacgctggac
      481 tactacctgc gactcgccga gcagatcgtg gacgcgggtg cgcacgtgat cggcatcaag
      541 gacatggccg gtctgctgcg tcctgcggcg gccgcgaagc tcgtcacggc gctgcgcgag
      601 cggttcgacc agcccgtgca cgtgcacacg cacgacaccg ccggcggcca gctggcgacc
      661 ctgctcgccg cgagcggagc cggagcggat gccgtggacg tggcatccgc tccgatgtcg
      721 ggcaccacca gccagccgtc cctgtcggcg ctgatcgcgg cgctcgcgca caccgagcgc
      781 gacaccggca tcgacctgca ggcggcatcg gacctggagc cgtactggga ggcgatgcgc
      841 cacctgtacc gcccgttcga gtcgggcctg ccgagcccca ccgggcgcgt gtaccgccac
      901 gagatccccg gcgggcagtt gtcgaacctg cgccagcagg cgatcgccct gggactggcc
      961 gacgacttcg agctcatcga ggacatgtac gccgcggcga acgacatcct cggccgggtg
     1021 ccgaaggtga ccccgtcctc caaggtggtc ggagatctgg cgctgcacct cgcagcggtc
     1081 aaggcggacc ccgccgactt cgccgcgaac ccggagcgct acgacatccc ggactccgtc
     1141 gtcggcttca tggccggtga gctgggcgac ctgcccggcg gatggccgga gccgttccgc
     1201 agcaaggtgc tcgccggtcg cgctgccaag gccgggatca cgcccatcac ggacgtccag
     1261 caggcggctc tcgacggcac gtccgcggag cgccgcggga tgctcaacga gctgctgttc
     1321 cccgcgccca cgaagatctt ccggcagaac cgagaggagt tcggcgacct gtccgtgctg
     1381 gacacctccg actacctcta cggactcgtc cccggtgtcg agcacgctgt cgagatcgaa
     1441 cgaggcgtgc gactgtacgt cgggctcgag gcgatcggcg aggcggaccc caagggcgtg
     1501 cgcaccgtca tgacgacgct gaacggccag ctgcggccgg tgtccgtacg cgatcgctcg
     1561 atcgtcgtcg acgcccacga gaccgagaag gcggacacct ccaagcctgg tcagatcgcc
     1621 gcgccgttct ccggcgcggt cacgctgaag gtcgaggagg gcgctgtcgt gaaggccggc
     1681 gatgcggtcg cgtcgatcga ggcgatgaag atggaggcgg ccatcacgac gtcggtcgac
     1741 ggggtggtgg agcggctcgc gatcgatcag acccagcagg tcgacgccgg tgatcttttg
     1801 gtcgtggtcc gcccggcgca ctaatcttgg gcgggcgcta agtctgcgcc ggagcttgga
     1861 gctggaaccg tgacgtcgaa gaacaacgtg gagcccgaag gggacgattc gctgggcgtg
     1921 ctcgacgaca ccacgtcgtc ggacacgatc gcgatcggcc tgcacgaggg caccgcgcag
     1981 gtcagcgtgg cgcttccgac cggcggcgag gacgatgatc tcgtcgacga cgaggtgatc
     2041 ggtggcgagg tcgtgtccga cctcatcatc gacacctcgg ccgtcgccga ccccgacgtg
     2101 ctcgcccgca tcagccagga tgcacccgag atcgtcatcg aactgcccgc cgaggagctg
     2161 ccggcagcgg ctgagacggc agaagaccgc cccgtcgacc cggagaccgt gaccgagctc
     2221 gtggtggagg cggaggagcc cggcatcgag gcggctgacg ccgtcgcgca tgccgatgac
     2281 atcgtcgacg cggacgtcgt acccgagtcc ggcggcgcgg atgccgaggt catcacggcc
     2341 ggcgatgacg agatcgtcgc ggatgcctct gacgaggccg aggcggatgc cggcgtcgaa
     2401 accatgaccg aggcggaggc gatcgcgggg gagaccgccg cgccgcctgc gcccaagccc
     2461 ggcatccggc cggagcccgt gcttctcccg cccctgaccg ccgcgcgcgg ccggaaggcg
     2521 gtgcgcaagc ccgcaaagtc gaagaagaag gccgagcagc cgaagcgcga gtcgcccgtc
     2581 gacgagacga cgccggccgc agcggagacc gcgcctgccg tcgagaacgt caccgtcgcg
     2641 acgcctgagc aggtcgcgaa gccagaggcc gcggcgaagc ccgaggcgac ggcgaagccc
     2701 gaggcggacg cgaagagcga ccagcccgtg ccgcctgcga agtccgagcc gcaggccgat
     2761 gagacgggct cggagcc
//
LOCUS       DATMSX010000195         8707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_50199,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000195 DATMSX010000000
VERSION     DATMSX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50199"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VN041_06015"
     CDS             <1..695
                     /locus_tag="VN041_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28636.1"
                     /translation="TSELRAKLAAAPTAGLSSQLRKRGLHSCFIDGVSANIAGSKIVG
                     TAKTLRFIPAREDLFRSHGGGYNAQKRAFDAVGEGEIIVIEARGDAGTGTLGDILALR
                     AKTRGAAGVVTDGGVRDFDAVTEIGLPVFSKGAHPSVLGRRHVPWEFDVTIACGGAAV
                     QPGDVIVGDGDGVIVIPFELAKEVADAALAQEDEDAWIADQVAAGHPVDGLFPMNAEW
                     RARYDERSGTGS"
     gene            692..1372
                     /locus_tag="VN041_06020"
     CDS             692..1372
                     /locus_tag="VN041_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28637.1"
                     /translation="MTTLVREGSKSEQAYAWIRSRIASHAFGPGYRLVLGTIADELGM
                     SVVPVREAIRRLEAEGLVTFERNVGAHVSLIDESEYAHTMQTLGIVEGAATALSAPLL
                     DADALDRAEAVNDRMLHLLGHFDAHTFTSLNQAFHSVLYEPCPNPHILDLVHRGWSRL
                     SGIRDSSFAFVPGRARHSVDEHTRILELIRGGADPLEIELAARDHRWRTMDAFLAARH
                     PHPTEEGR"
     gene            1372..2892
                     /gene="hpaE"
                     /locus_tag="VN041_06025"
     CDS             1372..2892
                     /gene="hpaE"
                     /locus_tag="VN041_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HWU28638.1"
                     /translation="MTDVYTPADLPAKIQHYIDGEFVDSLDGDTFEVLNPVTNETYVH
                     AAAGKKADIDRAVAAARRAFTEGPWPRMLPRERSRVLHRIADIVESRDARLAELESFD
                     SGLPITQALGQARRAAENFRFFADLIVAQADDAFKVPGKQMNYVNRKPIGVAGLITPW
                     NTPFMLESWKLGPALATGNTVVLKPAEFTPLSASLWAGIFEEAGLPKGVFNLVNGLGE
                     DAGDALVKHPDVPLISFTGESRTGQIIFGNSAPFLKGLSMELGGKSPAVVFADADLDT
                     AVDACVFGVYSLNGERCTAGSRIIVERAIYDEFVERFATMAKRVKVGLPHDPTTEVGA
                     LVHPEHYEKVMSYVEIGKTEGRLIAGGGRPEGFPTGNYVAPTAFADVSPDARIFQEEI
                     FGPVVAITPFDTDEEALSLANNTKYGLAAYIWTNDLKRAHNFAGAVEAGMVWLNSNNV
                     RDLRTPFGGVKASGLGHEGGYRSIDFYTDQQSVHITLGAPHNPAFGKQDLDDPDPR"
     gene            2929..4110
                     /gene="hpaD"
                     /locus_tag="VN041_06030"
     CDS             2929..4110
                     /gene="hpaD"
                     /locus_tag="VN041_06030"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HWU28639.1"
                     /translation="MSNNPASAAHPGKTLTSSGFYVSQDTEIICENPTPTPTSTPPDV
                     LRCAYMELVVTDLAASREFYVDVLGLYVTEEDDQAIYLRSTEEFIHHNLVLRKGEVAA
                     VAAFSYRVRSAEDLDRAVAFYTELGCDVRRAPSGFTKGIGDSVRVVDPLGFPYEFFHA
                     TDHVERLSWRYDLHKPGELVRLDHFNQVTPDVPRAVKYMQDLGFRVTEDIQDEEGTVY
                     AAWMRRKPTVHDTAMTGGDGPRMHHVCFATHEKHNILAICDKLGALRRSDAIERGPGR
                     HGVSNAFYLYLRDPDGHRVEVYTQDYYTGDPDNPVITWDVHDNQRRDWWGNPVVPSWY
                     TDASLVLDLDGNPQPVRARTDSSEMAVTIGADGFSYTRPDDEAEMPEWKQGEYKLGHQ
                     L"
     gene            4107..4895
                     /locus_tag="VN041_06035"
     CDS             4107..4895
                     /locus_tag="VN041_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU28640.1"
                     /translation="MTLPAETITALAAELAEAERTRGIMPRITARYPDATIEDSYAIQ
                     GVWRDAQLAAGRTLVGRKIGLTSKAMQQATGITEPDYGVMFDDTVYATGSEIPYDHFS
                     NVRIEVELAFALKAPLEGPDCTLEDALAAIDYAVPALEVLNSHIELEGRTIVDTISDN
                     AAYGAMVLGDVHMHPDEIDLRWVPGVLSRNGQIEETGVAAGVLGHPATGVAWLANKFH
                     QHGQRLEAGEIILAGSFTRPMWVQRGDTVLCDYREMGTIECRFV"
     gene            4880..5683
                     /locus_tag="VN041_06040"
     CDS             4880..5683
                     /locus_tag="VN041_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWU28641.1"
                     /translation="MPLRLDPTFRDRLAESHRPLIGMWLSTGSPLIAEICAGSGVDWL
                     LIDMEHGPNTLTTVQQQLQVIAAYPVTTVVRVPFNDAVIIKQVLDAGAQNLLVPMVAS
                     AEEARAAVAAVRYPPEGIRGVGSALARSARWGRVSGYVPNASQTISLIVQVESADAVE
                     RAAEIAGVDGVDGVLVGPADLSASMGLPGQQGHPDVVAGVHRVFEAAHAAGKKAGVNA
                     FDPAGADAYLEAGADFLSASADVTILARGSEALAARFIPASDEAGADSY"
     gene            5816..7060
                     /locus_tag="VN041_06045"
     CDS             5816..7060
                     /locus_tag="VN041_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28642.1"
                     /translation="MTRGLAAPRLAAMGDRNFRWFFLARAITMITGSMSSIALAFAVL
                     EIDNHPVSLSLVLTAFTVANVVFVLFGGVVADRLPRALVIQACYVLDLLSIGTIAYLL
                     FTHTATIPVMVALAAVNGASTAFVLPAMQGIIPQLTRPETLQQANAMLSFVRSAVTIG
                     GPVIAGILVTTAGPAWAMVVQLIGWLSAIPILAMVKLPPPVGDGRFSLFADLRIGWTE
                     FWSRSWLYAVVLAFMIMNAIHVGAWGVVGPYIAKNDERLGIAGWGWVVSAEGVGVLLM
                     TLVLMWFPLRRPLRMGMIGISLFAIPLTMLGAHPAVVLVAIAALLAGAGAEVFSTGWS
                     VAMMENIPTDKLSRVSSYDMLGSFVVMPIGTLVYGWLITRADPAVVLVTSGILYAVIA
                     VGTAFVPSVWRMGRVEAATVRG"
     gene            complement(7106..8470)
                     /locus_tag="VN041_06050"
     CDS             complement(7106..8470)
                     /locus_tag="VN041_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HWU28643.1"
                     /translation="MPTTQTRAEKRSVMRPINRFLERWVPSALTFAIVLTLVVAVLAM
                     TLTRTSPVDVVKSWGDGLAGLLAFMTQMCLILLLGYMLANTRPVRKLLRRLARVPGTA
                     AAAYIFVFVVAAVASLITWGLGLIVGTLLAREIAVVARERGIKVHFPLLVAAGYSGFV
                     VWHMGYSGSGPLTAATPGSFLAPSLGDKIIPVSQTTFSSWNMLAALGVIVVCGVLFFL
                     IRPRKDDPVYELPVHVGSEDDDAADDEVVTPADRIDASRILTLLLGLGLVAYLVVHFV
                     QGGTLTLDIVNWSFLALILLIVRNPFELIHLTKKAASNVGEILLQFPLYAGILGIMAG
                     SGLIKLFSDAFVSIATPVTFGVLALLSAGIVNFFVPSGGGQFAVQGPIMLDAANQLGV
                     DPSIAIMAVAYGDQWTNMIQPFWALPVLAIAGLKMRDILGYTFVTLLGSGVVLAAALL
                     IVSL"
     gene            8565..>8707
                     /locus_tag="VN041_06055"
     CDS             8565..>8707
                     /locus_tag="VN041_06055"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28644.1"
                     /translation="MTFETLRALADRAALFTALRQDQLMTATDALGEHRWDVDLAAGT
                     FTFA"
BASE COUNT         1410 a         3094 c         2908 g         1295 t
ORIGIN      
        1 tcacctcgga gctgcgcgcc aagctcgcgg cggcgccgac ggcgggcctg tccagccagc
       61 tgcgcaagcg cggcctgcac tcctgcttca tcgacggggt gtccgcgaac atcgccggca
      121 gcaagatcgt cggcaccgcc aagacgctgc gcttcatccc ggcccgcgag gatctgttcc
      181 ggagccacgg cggcggctac aacgcgcaga agcgcgcctt cgacgcggtc ggcgagggcg
      241 agatcatcgt gatcgaggcc cgcggcgatg ccggcaccgg aactctcggc gacatcctgg
      301 ccctgcgcgc caagacccgc ggagccgcgg gcgtcgtcac cgacggcggt gtgcgcgact
      361 tcgatgccgt caccgagatc ggtctgccgg tgttctcgaa gggggcccac ccgtccgtgc
      421 tgggccgccg ccatgtgccg tgggagttcg acgtcacgat cgcctgcggc ggcgccgccg
      481 tgcagcccgg cgacgtgatc gtgggagacg gcgacggcgt catcgtgatc ccgttcgagc
      541 tcgcgaagga ggtcgccgac gcggctctgg cccaggagga cgaggacgcc tggatcgccg
      601 atcaggtcgc cgccgggcat cccgtcgacg ggctgttccc gatgaacgcc gagtggcgcg
      661 cccggtatga cgagaggtcg ggcaccggat catgaccacc ctggtccgcg agggcagcaa
      721 gtcggaacag gcctacgcct ggatccgctc gcgcatcgcc tcgcacgcct tcggccccgg
      781 gtaccgcctg gtgctcggca cgatcgccga cgaactcggc atgagcgtgg tccccgtgcg
      841 ggaagccatc cgccgcctcg aggccgaggg tctggtcacg ttcgagcgca acgtcggcgc
      901 gcacgtctcg ctcatcgacg agagcgagta cgcgcacacc atgcagaccc tgggcatcgt
      961 cgaaggcgcc gccaccgcac tgtccgctcc cctgctggac gccgatgcgc tcgaccgcgc
     1021 cgaagccgtc aacgaccgga tgctgcacct gctgggccac ttcgacgcgc acacgttcac
     1081 ctcgctgaac caggcgttcc actcggtgct ctacgagccc tgcccgaacc cgcacatcct
     1141 cgacctggtg caccgcggat ggagccggct ctccggcatc cgcgactcct cgttcgcgtt
     1201 cgtccccggc cgcgcccggc actcggtgga cgagcacacc cgcatcctcg agctgatccg
     1261 cggcggcgcc gacccgctcg agatcgagct cgccgcccga gaccaccgct ggcgcaccat
     1321 ggacgcgttc ctggcggccc gccaccccca ccccaccgaa gaaggacgat gatgaccgac
     1381 gtgtacaccc cggccgacct gcccgcgaag atccagcact acatcgacgg cgagttcgtc
     1441 gactccctcg acggcgacac cttcgaggtg ctcaacccgg tcacgaacga gacctacgtg
     1501 cacgccgctg ccggcaagaa ggccgacatc gatcgcgcag tcgccgccgc ccgtcgtgcg
     1561 ttcaccgagg gcccgtggcc gcggatgctg ccgcgcgagc gctcgcgcgt gctgcaccgc
     1621 atcgccgaca tcgtcgagtc gcgcgacgcg cgcctcgccg agctggagtc gttcgactcc
     1681 ggactgccca tcacgcaggc cctgggccag gctcgccgcg ccgccgagaa cttccgcttc
     1741 ttcgccgacc tgatcgtcgc gcaggccgat gacgccttca aggtgcccgg caagcagatg
     1801 aactacgtca accgcaagcc gatcggcgtc gcggggctca tcacgccgtg gaacaccccg
     1861 ttcatgctcg agtcctggaa gctcggcccc gccctggcca ccggcaacac ggtcgtgctc
     1921 aagcccgccg agttcacgcc gctgtccgca tcgctgtggg cgggcatctt cgaagaggcg
     1981 ggcctcccga agggcgtgtt caacctcgtc aacggactcg gtgaggacgc cggagacgcc
     2041 ctggtgaagc atccggacgt cccgctgatc tcgttcacgg gtgagagccg caccgggcag
     2101 atcatcttcg gcaactcggc gccgttcctg aagggcctgt cgatggagct cggcggcaag
     2161 agccccgcgg tcgtcttcgc ggatgccgat ctcgacaccg cggtcgacgc ctgcgtcttc
     2221 ggcgtgtact ccctgaacgg cgagcgctgc acggcgggct cgcgcatcat cgtcgagcgc
     2281 gcgatctacg acgagttcgt cgagcgcttc gccaccatgg ccaagcgcgt gaaggtgggc
     2341 ctcccccacg acccgaccac cgaggtcggc gcgctcgtgc accccgagca ctacgagaag
     2401 gtgatgagct acgtcgagat cggcaagacc gagggtcgcc tgatcgccgg cggcgggcgc
     2461 cccgagggct tccccaccgg caactacgtc gccccgaccg ccttcgccga cgtgtctccg
     2521 gatgcccgga tcttccagga ggagatcttc gggccggtcg tcgcgatcac gcccttcgac
     2581 acggacgagg aggcgctgtc cctcgcgaac aacaccaagt acggtctcgc cgcctacatc
     2641 tggaccaacg acctcaagcg cgcgcacaac ttcgcgggcg ccgtggaggc cggcatggtg
     2701 tggctgaact cgaacaacgt gcgcgacctg cgcaccccgt tcggcggcgt gaaggcgtcg
     2761 ggtctgggcc acgagggcgg ctaccgctcg atcgacttct acaccgatca gcagagcgtg
     2821 cacatcaccc tcggcgcccc gcacaacccc gccttcggca agcaggacct ggacgacccg
     2881 gacccgcgct gacccgccgc ccgatttcag aagagcaagg acgctgacat gagcaacaac
     2941 ccggcctctg ccgcccaccc cggcaagacc ctcacctcct ccggcttcta cgtgagccag
     3001 gacacggaga tcatctgcga gaaccccacg ccgacgccga cgagcacccc gcccgacgtg
     3061 ctgcgctgcg cgtacatgga gctggtcgtg accgaccttg cggcgtcccg cgagttctac
     3121 gtcgacgtgc tcggcctgta cgtgaccgag gaggacgacc aggcgatcta cctgcgctcc
     3181 accgaggagt tcatccacca caacctcgtg ctgcgcaagg gcgaggtcgc cgcggtcgcc
     3241 gcgttctcgt accgcgtgcg ctcggctgag gacctcgacc gcgcggtcgc gttctacacg
     3301 gagctgggct gcgacgtgcg ccgcgcgccg tccggcttca cgaagggcat cggcgactcg
     3361 gtgcgcgtcg tcgacccgct cggcttccca tacgagttct tccacgcgac cgatcacgtc
     3421 gagcggctgt cgtggcgcta cgacctgcac aagccgggcg agctcgtgcg gctggaccac
     3481 ttcaaccagg tgacccccga cgtgccccgc gccgtgaagt acatgcagga cctgggcttc
     3541 cgcgtcacgg aggacatcca ggacgaggag ggcaccgtct acgccgcgtg gatgcgccgc
     3601 aagcccaccg tgcacgacac cgccatgacc ggcggtgacg gaccgcgcat gcaccacgtg
     3661 tgcttcgcga cgcacgagaa gcacaacatc ctggccatct gcgacaagct cggtgcactg
     3721 cgccgctcgg acgccatcga gcgcggtccc ggccgtcacg gcgtcagcaa cgcgttctat
     3781 ctctacctgc gcgaccccga cgggcaccgc gtggaggttt acacgcagga ctactacacc
     3841 ggcgaccccg acaacccggt catcacctgg gacgtgcacg acaaccagcg ccgcgactgg
     3901 tggggcaacc cggtcgtgcc gagctggtac acggatgcct ccctcgtgct cgacctggac
     3961 ggcaacccac agccggtgcg cgcccgcaca gacagcagcg agatggccgt gaccatcggc
     4021 gccgacggct tctcatacac ccgtcccgac gacgaggccg agatgccgga gtggaagcag
     4081 ggcgagtaca agctgggtca tcagctgtga ctcttcccgc cgagacgatc accgcgctgg
     4141 ccgccgagct ggccgaggcc gagcgcaccc gcggcatcat gccgcgcatc accgctcgat
     4201 acccggatgc cacgatcgag gactcctatg cgatccaggg cgtctggcgc gacgcgcagc
     4261 tggcggccgg ccgcacgctc gtgggccgca agatcggcct gacctcgaag gccatgcagc
     4321 aggcgaccgg gatcaccgag ccggactacg gcgtgatgtt cgacgacacg gtgtacgcca
     4381 ccggatcgga gatcccgtac gaccacttct cgaacgtgcg catcgaggtg gagctggcct
     4441 tcgccctgaa ggcgcccctc gagggcccgg actgcacgct cgaggacgcc ctcgccgcga
     4501 tcgactacgc cgtgccggca ctggaggtgc tgaactccca catcgaactg gagggacgca
     4561 ccatcgtcga cacgatcagc gacaacgccg cctacggcgc gatggtgctg ggcgatgtgc
     4621 acatgcaccc cgacgagatc gacctgcgct gggtgcccgg ggtcctctcc cgcaacggcc
     4681 agatcgagga gaccggcgtc gccgcaggcg tgctggggca tcccgccacc ggcgtggcct
     4741 ggctggcgaa caagttccac cagcacgggc agcggctgga ggccggggag atcatcttgg
     4801 caggatcgtt cacgcggccc atgtgggtgc agcgcggcga caccgtgctg tgcgactacc
     4861 gcgagatggg gaccatcgaa tgccgcttcg tctagacccg accttccgcg accggctcgc
     4921 cgagtcccac cggccgctga tcggcatgtg gctgagcacc ggttctcccc tgatcgccga
     4981 gatctgcgcc ggctcgggtg tcgactggct gctgatcgac atggagcacg ggcccaacac
     5041 cctgaccacc gtccagcagc agctgcaggt gatcgccgcg tacccggtga ccacggtcgt
     5101 gagggtgccg ttcaatgacg cggtgatcat caagcaggtc ctggatgcgg gcgcgcagaa
     5161 cctgctcgtg cccatggtgg cctccgccga ggaggcacgg gcggccgtcg ccgccgtgcg
     5221 ctacccgccg gagggcatcc ggggcgtcgg ttccgcgctc gcgcgcagcg cgcggtgggg
     5281 ccgggtgagc ggatacgtgc cgaacgcctc gcagacgatc tcgctgatcg tgcaggtcga
     5341 gtcggctgat gccgtggaac gggccgccga gatcgccggg gtcgacggcg tcgacggcgt
     5401 gctggtgggc ccggccgacc tgtcggcctc gatgggcctg cccggtcagc aggggcatcc
     5461 ggatgtcgtc gccggcgtgc accgcgtgtt cgaggccgcg catgccgcag gcaagaaggc
     5521 cggcgtgaac gcgttcgacc cggcgggtgc cgacgcctac ctcgaggccg gcgccgactt
     5581 cctctcggcg agcgcagatg tgaccatcct cgctcggggc tccgaggctc ttgcggcccg
     5641 gttcatcccg gcatccgacg aagcgggcgc cgactcgtac tgacgcccgt cacagctccg
     5701 gagaaccgac gacagtccgc cgcccgacgg gggattccgt gccgttcgcg acaggttctc
     5761 cggagtcgtg gccgctgtgg ttggatgaac ggatgcacag cgccgagacc gaccgatgac
     5821 gcgcggcctc gccgctccgc gcctggcggc gatgggcgac cgcaacttcc ggtggttctt
     5881 cctcgccagg gcgatcacga tgatcacggg ctccatgtcg tcgatcgcgc tggcgttcgc
     5941 ggtgctcgag atcgacaacc acccggtcag cctgtcgctg gtgctcaccg ccttcacggt
     6001 cgcgaacgtc gtcttcgtgc ttttcggcgg tgtcgtggcc gaccggctcc cccgcgcgct
     6061 ggtgatccag gcctgctacg tgctcgatct gctctcgatc ggcacgatcg cctatctgct
     6121 gttcacgcac accgccacca tccccgtgat ggtggccctc gcagcggtca acggcgcgtc
     6181 gaccgcgttc gtgctccccg cgatgcaggg catcatcccg cagctcaccc ggccggagac
     6241 tctgcagcag gccaacgcga tgctctcgtt cgtgcgctcc gcggtcacga tcggcggacc
     6301 ggtgatcgcc ggcatcctgg tcacgaccgc gggccccgcc tgggcgatgg tcgtgcagct
     6361 gatcggctgg ctctcggcca tcccgatcct ggcgatggtg aagctgccgc cacccgtcgg
     6421 cgacgggcgg ttctcgctgt tcgccgacct gcggatcggc tggaccgagt tctggagccg
     6481 gtcctggctg tacgcggtcg tgctcgcgtt catgatcatg aacgcgatcc acgtcggcgc
     6541 atggggcgtc gtcggtccct acatcgccaa gaacgacgag cggctgggca tcgccggctg
     6601 gggctgggtg gtcagcgccg agggtgtcgg tgtgctgctg atgacgctgg tcctgatgtg
     6661 gttccccctc aggcgccctc tgcggatggg gatgatcggc atcagcctgt tcgcgatccc
     6721 gctgacgatg ctgggcgcgc acccggcggt cgtgctcgtc gcgatcgcgg ctctgctggc
     6781 cggcgcgggc gcggaggtgt tcagcacggg ctggagcgtg gcgatgatgg agaacatccc
     6841 caccgacaag ctcagccgcg tctccagcta cgacatgctg ggcagcttcg tggtgatgcc
     6901 gatcggcacc ctcgtgtacg gctggctgat cacgcgggcc gatccggccg tggtgctggt
     6961 cacctccggc atcctgtacg cggtcatcgc cgtcggcacc gcgttcgtgc cgagcgtgtg
     7021 gcggatgggc cgcgtcgagg cggcgaccgt ccggggctag tccgaagatc gccgcctcgg
     7081 atgcgccgag ccggatcggg acggatcaga gactgacgat gagaagcgcg gctgcgagca
     7141 cgacgcccga ccccagcagg gtcacgaagg tgtacccgag gatgtcgcgc atcttcagac
     7201 ccgcgatcgc cagcacgggc agcgcccaga acggctggat catgttggtc cactgatcgc
     7261 cgtacgccac ggccatgatc gcgatcgacg gatcgacgcc cagctggttc gcggcgtcga
     7321 gcatgatcgg tccctgcacg gcgaactggc ctccgccgga gggcacgaag aagttcacga
     7381 tgccggccga gaggagcgcc agcactccga acgtcacggg cgtggcgatc gacacgaagg
     7441 catccgagaa cagcttgatc aggccactgc ccgccatgat gccgaggatg ccggcgtaga
     7501 gcgggaactg gagcaggatc tcaccgacgt tcgacgccgc cttcttggtg aggtggatca
     7561 gctcgaacgg gttgcgcacg atcagcagga tcagtgccag gaacgaccag ttgacgatgt
     7621 cgagggtgag cgtcccgccc tgcacgaagt gcacgaccag ataggcgacc agcccgagtc
     7681 ccagcagcag ggtgaggatg cgcgaggcgt caatacggtc cgcgggagtg acgacttcgt
     7741 catcggcggc gtcgtcgtcc tccgaaccga cgtgcaccgg aagctcgtac accgggtcgt
     7801 ccttgcgcgg gcggatcagg aagaacagca caccgcagac cacgatcacc ccgagagcgg
     7861 ccagcatgtt ccagctcgag aacgtggtct ggctgaccgg gatgatcttg tcgcccagac
     7921 tcggcgcgag gaaggaaccc ggcgtagccg cggtcagcgg gcctgacccg gagtacccca
     7981 tgtgccagac gacgaagccg gagtatccgg cggcgaccag cagcgggaag tgcaccttga
     8041 tgccccgctc tcgtgccacg accgcgatct cacgggccag cagcgtaccg acgatcagac
     8101 cgagacccca ggtgatgagg ctcgcgactg cggcgacgac gaaaacgaag atataggccg
     8161 cggcggcggt gccgggcacg cgggccagcc ggcgcagcag cttccgcacg gggcgggtgt
     8221 tggcgagcat gtaacccagc agcaggatca ggcacatctg cgtcatgaac gccaacaggc
     8281 ccgccaggcc gtcgccccag ctcttcacga cgtcgaccgg gctggtgcgc gtgagggtca
     8341 tcgcgaggac ggccacgacc agggtcagga cgatcgcgaa ggtgagcgcg gagggcaccc
     8401 agcgctccag gaatcggttg atcgggcgca tcacactgcg cttctcagct cgtgtctgcg
     8461 ttgtgggcat gacagaaact ccttcgtctc ggtgatacct cgaccatagc gatctcacag
     8521 ccagcgcctt ggaactgcgc ggcttccggg gccagactgg acgcatgacc ttcgagacgc
     8581 tgcgagccct cgccgaccgc gccgccctgt tcaccgccct gcgccaggac cagctgatga
     8641 ccgcgaccga cgcgctcggc gagcaccgct gggacgtcga cctcgccgcc ggcaccttca
     8701 cgttcgc
//
LOCUS       DATMSX010000196         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_173380,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000196 DATMSX010000000
VERSION     DATMSX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173380"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VN041_06060"
     CDS             complement(<1..612)
                     /locus_tag="VN041_06060"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HWU28645.1"
                     /translation="MTRLDGARPVAVLYTGGTFGMVTTPRGLAPDPHLREQIESLVAR
                     MNTGESAPTWTYETTARAIDSAELQPGSMAELADGIREIVEAQHPVGIVVIHGTDTMA
                     YSAAFAAFALADVDVPVVFTGAQVPLGMPGSDAPDNFAQAFAAASEETPAGTWIAFGG
                     HLLPAVRTTKRSSRSLDAFAALRALAPGSSGAPPELTAALTRAA"
     gene            745..1113
                     /locus_tag="VN041_06065"
     CDS             745..1113
                     /locus_tag="VN041_06065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWU28646.1"
                     /translation="MTDRTKPEFDAPTGPAPTELVIRDIIVGDGDEAKPGDTVTVHYA
                     GVEHDSGEEFDSSWGRGETIQFPLRGLIQGWQEGIPGMKVGGRRELTIPPHLAYGPAG
                     AGHFLSGKTLIFIIDLVAVG"
     gene            1309..1857
                     /locus_tag="VN041_06070"
     CDS             1309..1857
                     /locus_tag="VN041_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWU28647.1"
                     /translation="MSIEIPGYRPGTYVLDPAHSEVTFSVRHMMISKVRGTFGVKEAT
                     LVAPENPLEAKVEAKVDVTSIDTKDENRDAHLRSADFFDVENHPSMEFVSTGTRVEDG
                     DFLVDGDLTIRGITKPVTFEFEFGGFGTDPYGNYKAGASAKTVINREDFGLTWNAALE
                     TGGVLVGKEITIGLDLQGALQA"
     gene            complement(1901..3193)
                     /locus_tag="VN041_06075"
     CDS             complement(1901..3193)
                     /locus_tag="VN041_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28648.1"
                     /translation="MQFSRGLLIDLSPLKISPAFTRMWIGSTLAGIGGQLTVVTVMLH
                     VFELTGSTFAVSMIAVAGLVPMILAGLYGGMLADAFDRRRVALISATVAFIATALLAT
                     LTWTHTETIWWLYGLSMTIAAANSVGMATRTAIVPRLIPLDHLAAASALNGVAFGLTV
                     MVGPAVAGLLVALTGFGWTYTIDVILMLSMFLGLWTLPALLPEGTIVRPGLASLVDGW
                     RFLRRAGNIRMQYLMDITAMTFGQPLALFPALGTVILGGGAFTTGILTAAVAVGTFAC
                     SLFSGRIVHYRWHGLGISRAVQAYGVAIALFGAVLLIGMLLPPASEQVPHVALIVAAC
                     VALALSGAADNVSSIYRNTMMQAAVPDTMRGRLQGVFIIVVAGGPRVGALYAGALATV
                     TSLWFPPLLGGFLVIGIVAVLARLSPRFRAYDALSPEA"
     gene            3335..>3436
                     /locus_tag="VN041_06080"
     CDS             3335..>3436
                     /locus_tag="VN041_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWU28649.1"
                     /translation="MALEADVKKAIIEEYATHPGDTGSPEVQVAMLTQ"
BASE COUNT          609 a         1147 c         1178 g          502 t
ORIGIN      
        1 cgccgctcgg gtgagtgcgg cggtcagctc gggcggcgcg ccggagctcc cgggggcgag
       61 tgctcgaagg gcggcgaagg cgtccaggct gcggctcgag cgcttcgtcg tgcgtaccgc
      121 aggaagcagg tgccctccga acgcgatcca ggtgcccgca ggcgtctcct cggatgcggc
      181 ggcgaacgcc tgcgcgaagt tgtcaggcgc atcgctccca ggcatgccca gcggtacctg
      241 tgcgcctgtg aacaccaccg ggacgtcgac atccgccagc gcgaaagccg cgaaggccgc
      301 gctgtaggcc atggtgtccg tgccgtggat cacgacgatc ccgaccggat gctgcgcttc
      361 cacgatctcg cggatgccgt cggcgagttc ggccatgctg ccgggctgga gctctgcgct
      421 gtcgatggcc cgggccgtcg tctcgtacgt ccacgtcgga gcggactcac cggtgttcat
      481 gcgcgcgacc aggctctcga tctgctccct caggtgcgga tccggcgcca ggccacgagg
      541 ggtcgtcacc atcccgaacg tgccgccggt gtagagcaca gcgaccgggc gtgctccgtc
      601 gagtcgggtc atggagcgct cctcgaggcc ttgctgatga cctcgcaatc ctattccgag
      661 gggagtcggg tgcgtgcgct gctgttcgct ctcggcgcgc gcggcgacgt tgaccgaggt
      721 gcgtggctcg ggtttgatgg aatcatgaca gatcgcacca agcccgagtt cgacgcaccg
      781 accggacccg cacccaccga gctcgtcatc cgcgacatca tcgtcggcga cggcgatgag
      841 gcgaagcccg gcgacaccgt caccgtgcac tacgccggcg tcgagcacga ctccggcgag
      901 gagttcgact cctcgtgggg gcgcggtgag accatccagt tcccgctgcg cggcctaatc
      961 cagggctggc aggagggcat ccccggcatg aaggtcggcg gtcgtcgtga gctgaccatt
     1021 ccgccgcacc tcgcgtacgg tcccgccggc gcaggccact tcctctccgg caagacgctc
     1081 atcttcatca tcgacctggt cgccgtaggc tgatcctgcg aggtcggtca gccctcggca
     1141 tcgacctggt cgccgtcggc tgaacctcga catcctcgaa gcccccgtcc gcatcctgcg
     1201 ggcgggggct ttctgcaaaa atcctgaaag ccgggaatgc attcaagtga atgcaagtta
     1261 tcctcattac ggcccacggg caccccacca cttcaaggag aagcagacat gtcgatcgag
     1321 atccccggct accgtcccgg cacctacgtc ctcgaccccg cgcacagcga ggtgaccttc
     1381 agcgtccgtc acatgatgat ctcgaaggtg cgcggcacct tcggcgtgaa ggaggcgacg
     1441 ctggtcgccc ccgagaaccc gctcgaggcc aaggtggagg cgaaggtcga cgtcacgtcg
     1501 atcgacacca aggacgagaa ccgcgacgcc cacctgcgct ccgcggactt cttcgatgtc
     1561 gagaaccacc cgtcgatgga gttcgtgtcg accggcaccc gtgtcgagga cggtgacttc
     1621 ctcgtcgacg gcgacctgac catccgcggc atcaccaagc cggtcacctt cgagttcgag
     1681 ttcggcggct tcggcaccga tccttacggc aactacaagg ccggcgcgtc cgcgaagacc
     1741 gtcatcaacc gcgaggactt cggcctcacc tggaacgccg cgctggagac cggtggcgtc
     1801 ctggtcggca aggagatcac catcggcctc gacctgcagg gtgccctgca ggcctgatcc
     1861 gcttccccct cacgagcccc gtggcccggc gcagtcagcg tcaggcttcg gggctcagtg
     1921 cgtcgtaggc gcggaagcgg gggctgagcc gtgcgagcac cgcgacgatc ccgatcacca
     1981 ggaagccgcc caacagggga gggaaccaca ggctggtcac cgtggcgagg gcaccggcat
     2041 acaatgcacc gacgcgggga ccgccggcga ccacgatgat gaagacgccc tgcaggcgcc
     2101 cgcgcatggt gtccgggacg gccgcctgca tcatcgtgtt ccggtagatc gagctgacgt
     2161 tgtccgcggc accggagagc gccagtgcga cgcaggcggc gacgatgaga gcgacgtggg
     2221 gcacctgctc cgatgccggc ggcaggagca tcccgatcag cagcaccgcg ccgaacagcg
     2281 cgatggcgac gccgtaggcc tgtactgcgc gggagatgcc cagcccgtgc cagcggtagt
     2341 gcacgatccg cccggagaac aggctgcagg cgaacgtgcc gaccgcgacc gccgccgtga
     2401 ggatgccggt ggtgaaggca ccgccgccga ggatcactgt tccgagtgcg gggaacaggg
     2461 cgaggggctg accgaaggtc atcgccgtga tgtccatcag gtactgcatc cggatgttcc
     2521 ccgcgcgccg caggaagcgc cacccgtcga ccagggaggc gagccccggg cgcacgatcg
     2581 tcccctccgg gaggagcgcg ggcagtgtcc acaggccgag gaacatcgac agcatcagga
     2641 tgacgtcgat cgtgtacgtc catccgaatc cggtgagcgc caccagcagg ccggcgaccg
     2701 ccggaccgac catcacggtc agcccgaagg cgacgccgtt gagggcggat gctgcggcga
     2761 ggtgatcaag ggggatcagt cgcggcacga tcgcggttcg cgtggccatc ccgacggagt
     2821 tcgccgcggc gatcgtcata ctcagcccgt acagccacca gatcgtctcg gtgtgcgtcc
     2881 aggtcagcgt cgccagcagc gcggtcgcga tgaaggcgac cgtcgcggag atgagcgcga
     2941 cgcggcgccg gtcgaaggca tccgccagca tccctccgta gaggcctgcg aggatcatgg
     3001 gcacgagacc tgcgacggcg atcatcgaca cggcgaaggt gcttccggtc agctcgaaga
     3061 cgtgcagcat cacggtcacg acggtgagct gcccgccgat gcccgcgagg gtcgagccga
     3121 tccacatgcg agtgaacgcc gggctgatct tcaaggggga gaggtcgatg agaagaccgc
     3181 gggagaactg cacctctcga ggctatcggc tgtgcgcgac cggggcgccg aagctggtag
     3241 gctatcgagg ttgccgttcg atcggccgcg gatgaagaga gcccacacat caggtgtcgg
     3301 gcgccgcgca acaagcagag aggggatcga atctatggca ctggaagcag acgtcaagaa
     3361 ggcgatcatc gaagagtacg cgacgcaccc cggtgacacc ggatcccccg aggtgcaggt
     3421 cgcgatgctg acgcag
//
LOCUS       DATMSX010000197         6679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_12230,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000197 DATMSX010000000
VERSION     DATMSX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12230"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VN041_06085"
     CDS             <1..678
                     /locus_tag="VN041_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWU28650.1"
                     /translation="VRGIGERLAERIVAARDEHGPFRDLHDLVRRTDATAAQLEALAT
                     AGAFECLGLSRREAIWLAGAAAEDRARFLPGTALAVQPPLFPDQSGYERLAADLWATG
                     VSTDDHPMAHLRDVLIERGVLTSQQLRTHESGRRIEVAGVVTHRQRPATASGITFLNL
                     EDEHGLVNVICSAGVWGRYRRVARDSPALIVRGILERSPEGVANVLADAFEDLRTGLA
                     HRSRDFR"
     gene            complement(716..1516)
                     /locus_tag="VN041_06090"
     CDS             complement(716..1516)
                     /locus_tag="VN041_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28651.1"
                     /translation="MTREYAGAVLELRDVHLRRSGRAILREVSFTVRTGQRWALLGPN
                     GAGKSTVLGFCGAQTHPSAGTVDVLGERIGRVDLQALRRRIGHVDPRHPLQSPLSATQ
                     VVLTGVTGTVELPMRWTPRSEEVARAHSALDAVGLSDRHEANWSTMSQGERGRALIAR
                     ALVMDPELLLLDEPTTGLDVAAREQFLETVDALVRREPELTSILVTHHLEELPESTTH
                     ALVISAGEVVAAGPVAEVLTTETISRAFRHPIEVTRHGRRWGARAVRS"
     gene            complement(1513..2208)
                     /locus_tag="VN041_06095"
     CDS             complement(1513..2208)
                     /locus_tag="VN041_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28652.1"
                     /translation="MAQVMRETLADRAAQLLLERIRSGEWALGERLPGETTLGPQLGV
                     GRSTVREAIRQLAAKGVLQTRQGAGVFVTALDLRSDWDDVLRRADILAVIETRTAIES
                     EAADLAARRRTQTDLRALRQALAGRDASRVAARGSRRTASIEGHVDADLALHRCIVVA
                     AHNPVLLDLYDSLTPRLRQAMIDMLRMRRSFGDDVDHEAHRDLVDAVVGRRSSEASAL
                     SRAHLTGLAEAIA"
     gene            complement(2247..2372)
                     /locus_tag="VN041_06100"
     CDS             complement(2247..2372)
                     /locus_tag="VN041_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28653.1"
                     /translation="MVSSSGLVSATGLDTSRLHDGEPIELGPVVATKEKTLVKHG"
     gene            2371..3813
                     /locus_tag="VN041_06105"
     CDS             2371..3813
                     /locus_tag="VN041_06105"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWU28654.1"
                     /translation="MSASITPASPVGTSEPPAPAWNRQRHSPMPSHRYSDVFSRVDVP
                     LRDDRLWPGRRLTAAPLWVPVDLRDGNQALAEPMSPQRKRRFFELMVTMGYKEIEVGY
                     PSASQTDYDFVRLLAETDLAPDDVTIVVFTPARRDLIERTAAAIRGIRNDVVIHLYTA
                     TAPIWRDVVLGRSRSELRELILGGGRDVLEVAGDLPNVRFEFSPEVFNQTEPDYVLEI
                     CDAMTSLWDASPDRPVIINLPATVEIATPNVYADQIEYMDRHLDRRDSVILSVHPHND
                     RGTGVACAELAMLAGAQRVEGCLFGNGERTGNVDLVTLALNLHTQGIDPMVDFSDIDG
                     IRGTVEECNRIEVHPRHPYAGSLVHTAFSGTHQDAIRKGLAEHRSRATAEGRSEAEIA
                     WRVPYLPIDPADIGRGYEAVIRVNSQSGKGGIAHLLQAAHGIELTREEQVELALRVQQ
                     HADATGGEVTADEVLGILRSRPARKAQPID"
     gene            complement(3819..4688)
                     /locus_tag="VN041_06110"
     CDS             complement(3819..4688)
                     /locus_tag="VN041_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28655.1"
                     /translation="MSWVEITEPRQRGELLAAAVGRAYRAKAAAERQDVPVPESAAGE
                     IWCTDDGSAAVWAIPREDEVVLADLHTTGQDPAAVWMLLDGLAAEAGWTGIWHFSSFS
                     GDAFMTALAESADARRVATKMRVEVAEVPAPAGIRLHPMDDADYAAFRDASDEEYAQE
                     RFESGADPTIEAARRTTAEQMLELLPEGLQTPGNRFWTVRDDDDRRAGILWLHLRENL
                     GFICDIAMDEDRRGQGLGTQTLRAAAEETARAGLDVLALNVFGSNPGARRLYEREGYR
                     ITESLWSVPIPRR"
     gene            complement(4685..4846)
                     /locus_tag="VN041_06115"
     CDS             complement(4685..4846)
                     /locus_tag="VN041_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28656.1"
                     /translation="MEKNRAEEPERPAAVDAEEALREADAAAGAAKAEANRWGGADKK
                     PAEPKDRFW"
     gene            complement(4892..5515)
                     /locus_tag="VN041_06120"
     CDS             complement(4892..5515)
                     /locus_tag="VN041_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU28657.1"
                     /translation="MREESAAGGIPVAGRRSVQTPDGLADADVYDVDYSDPEQVLVDR
                     EGMPPADVRQITELMSSLGELREAEEQLSEASRRYMRLNDTDMRALHYLIVCAHRGIL
                     ATPGGIAHHLRISSASTTKLLDRLERGGHIVRAPHPTDRRALAISITPQTHQAAMETV
                     GRQQAKRFYAAARLTSAEREVVIRFLRDMAREIAPGDEAWTQQHAKD"
     gene            5612..>6679
                     /locus_tag="VN041_06125"
     CDS             5612..>6679
                     /locus_tag="VN041_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU28658.1"
                     /translation="MSHSTEKTHRTWPRVLISSALVLLWLVGAGLGGPLFGKIDEVSS
                     NDATAYLPDSAAATEVQRSLGDFLGSDSIPAVVVFSSDAKIDDALKDELAARLEDATA
                     AEGVKEGSSPVLVSDDGLAAQAFVPIDADAEVGEVTAALSADLRKSVPDGVTVHITGP
                     AGFTADLLAGFSGIDGILLLVALLAVLVILVIVYRSLLLPLIVLCTSLFALCVALLVV
                     WWAAKLGILLLSGQTQGILFILVIGAATDYSLLLVARFREELHTTQNRWAAVRAAWKG
                     AFEPILASGGTVIAGLLCLLLSDLKSNSTLGPVAAIGIVFAMLAALTLLPALLGLAGR
                     AAFWPRRPAFEPGLTPQDDPLQ"
BASE COUNT          949 a         2402 c         2254 g         1074 t
ORIGIN      
        1 gtccgcggca tcggcgagag gcttgcggag cggatcgtgg ccgcacgcga cgagcacggg
       61 ccgttccgcg atctgcatga tctggttcgg cgcacggatg ccacggctgc gcagctcgag
      121 gcgctcgcca ccgccggcgc cttcgagtgt ctcggcctga gcaggcgcga ggcgatctgg
      181 ctggcggggg cggccgccga ggatcgcgcg cgattcctgc ccggaacggc gctcgccgtg
      241 cagccgccgc tgttccccga ccagtcgggg tacgagcgcc tggcggctga tctgtgggcc
      301 accggggtct ccaccgacga ccacccgatg gctcacctgc gggatgtcct catcgagcgc
      361 ggtgtgctca cctctcagca gctgcgcacg cacgagtccg gccgccgtat cgaggtggcg
      421 ggtgtggtca cgcaccggca gcggccggcg actgccagtg gcatcacctt cctgaacctg
      481 gaggacgagc acgggctggt caacgtgatc tgctccgccg gcgtctgggg gcgctatcgg
      541 cgggtcgccc gcgactcccc tgcgctcatc gtccgcggca tcctggagcg ttcccctgag
      601 ggcgtcgcga atgtgctcgc cgacgccttc gaggatctgc gcaccggcct cgcgcaccgc
      661 tcccgggact tccgatgagc ggcgcgagcg gcgccggcat cgcgacaggt tgtgttcagc
      721 tgcgtaccgc tctcgcaccc cagcgtcggc cgtgccgggt cacctcgatc ggatgccgga
      781 aggcgcgcga gatcgtctcc gtcgtgagca cctcggccac cggccccgcc gcgaccacct
      841 cgccggccga gatgacgagt gcatgcgtcg tggactcggg cagctcctcg agatggtgcg
      901 tgacgaggat gctggtcagc tcgggctctc ggcgcaccag ggcatccacc gtctcgagga
      961 actgctcgcg cgccgcgaca tcgaggccgg tcgtgggctc gtccagcagc agcagctcgg
     1021 ggtccatgac gagagcgcgc gcgatcagcg cacgcccgcg ttcgccctgc gacatcgtgg
     1081 accagttcgc ctcgtgacgg tcactcagac cgacggcgtc gagtgcggag tgcgctcgtg
     1141 ccacctcctc ggaccgcggt gtccacctca tcggcagctc gacggtcccg gtcactccgg
     1201 tgagcaccac ctgcgtcgcc gagagcggcg actggagcgg atgcctcgga tcgacgtgtc
     1261 cgatgcgtcg gcgcagcgcc tgcagatcga cgcgaccgat ccgctccccc agcacgtcca
     1321 ccgtccccgc gctgggatgc gtctgcgcac cgcagaagcc gagcacggtg ctcttccccg
     1381 ccccgttcgg accgagcaga gcccagcgct gtccggtccg caccgtgaac gagacctcgc
     1441 gcaggatggc ccgtccgctc cggcgcaagt ggacgtcccg cagctccagc accgcacccg
     1501 cgtactcgcg ggtcacgcga tcgcctcggc gagtccggtg aggtgtgccc ggctgagcgc
     1561 ggatgcctcg gatgacctgc ggcccaccac cgcgtcgacg agatcccggt gagcctcatg
     1621 gtcgacgtca tcgccgaacg accggcgcat cctgagcatg tcgatcatcg cctgacgcag
     1681 tcgcggtgtg aggctgtcgt acaggtcgag caggacgggg ttgtgcgcgg cgacgacgat
     1741 gcacctgtgc agcgccaggt cggcatcgac gtgaccctcg atcgatgcgg tgcggcgcga
     1801 gccacgggct gcgacacgag atgcatcgcg ccccgccagc gcctgccgca gggcgcggag
     1861 gtccgtctgc gtgcgtcgtc gggccgcgag atccgccgcc tcgctctcga tcgcggtgcg
     1921 cgtctcgatc acggcgagga tgtcggcccg acgcagcacg tcgtcccagt cgctgcgcag
     1981 gtcgagggct gtgacgaaga cacctgcgcc ctgccgggtc tgcagcacgc ccttcgcggc
     2041 gagctggcgg atcgcctcgc gcaccgtgga gcggcccact ccgagctggg gcccgagtgt
     2101 cgtctcgccg ggcaaccgct ccccgagcgc ccactccccg ctccggatcc gttcgagcag
     2161 cagctgcgcc gcccggtcgg ccagtgtctc gcgcatcacc tgtgccatcg caacccatct
     2221 gcttgtctga ggagttgtgt tacagtctag ccatgcttca ccagggtctt ctccttcgtc
     2281 gccacgacgg ggcctagttc gatcggctcc ccgtcgtgga gcctcgacgt gtccaggccg
     2341 gtcgcactca ccagaccgga agaagagacc atgtccgcat ccatcactcc cgcctccccc
     2401 gtcggcacct ccgagccgcc cgcaccggcg tggaaccggc agcggcactc gccgatgccc
     2461 tcgcaccgat actccgacgt cttctcccgc gtggacgtcc ccctgagaga cgatcggctg
     2521 tggccgggac ggcgtctgac agcggcgccg ttgtgggtcc ccgtcgacct gcgcgacggc
     2581 aatcaggccc tcgccgaacc catgagcccc cagcgcaagc gccgcttctt cgagctcatg
     2641 gtcacgatgg gctacaagga gatcgaggtg ggctacccgt ccgcctcgca gaccgactac
     2701 gacttcgtgc ggctgctcgc cgagaccgat ctcgcacccg acgatgtcac gatcgtggtc
     2761 ttcacgcccg cgcgccgcga tctcatcgag cgcacggcgg ccgcgatccg cgggatccgg
     2821 aatgacgtgg tcatccacct ctacaccgcc accgccccga tctggcgcga tgtcgtgctg
     2881 ggccggagcc gaagtgagct gcgggagctg atcctgggcg gcggccgaga cgtgctcgaa
     2941 gtcgcaggcg acctcccgaa cgtccgcttc gagttctccc ccgaggtgtt caatcagacc
     3001 gagcccgact acgtgctcga gatctgcgac gcgatgacct cgctgtggga cgcgtcaccc
     3061 gatcgtcccg tgatcatcaa cctccccgcc acggtggaga tcgccacgcc gaacgtctac
     3121 gccgatcaga tcgagtacat ggatcgtcat ctcgatcgcc gcgactccgt gatcctgtcg
     3181 gtgcatccgc acaacgaccg gggcaccggg gtcgcctgcg cggaactggc gatgctggcc
     3241 ggggctcaac gcgtggaggg ctgtctcttc ggcaacggcg agcgcaccgg gaatgtcgat
     3301 ctcgtgacgc tcgccctgaa cctgcacacc cagggcatcg acccgatggt cgacttctcg
     3361 gacatcgatg ggatccgcgg cacggtcgag gagtgcaatc gcatcgaggt gcacccgcgg
     3421 catccgtacg ccggctcgct ggtgcacacg gctttcagcg gcacgcacca ggacgcgatc
     3481 cgcaaaggac tggcggagca ccgcagccgc gcgactgcgg aggggcggag cgaggcagag
     3541 atcgcgtggc gcgtgcccta cctgccgatc gatcccgccg acatcggccg ggggtacgag
     3601 gccgtgatcc gcgtcaactc gcaatcgggc aagggtggca tcgcgcatct tctgcaggcc
     3661 gcccatggca tcgagctcac gcgggaagag caggtcgagc tggcgctgcg cgtgcagcag
     3721 cacgccgatg cgacaggcgg agaggtcacc gccgacgagg tgctggggat cctgcggagc
     3781 cgcccggcgc ggaaggccca gccgatcgac tgagaccgtc agcgccgcgg aatggggacg
     3841 gaccagagcg actcggtgat gcgatatccc tcgcgctcgt acagccgtcg tgcgcccggg
     3901 ttgctgccga agacgttcag cgccagcacg tcgagacccg cgcgggccgt ctcctccgcc
     3961 gccgcgcgca gcgtctgcgt gccgagtccc tgcccgcgac ggtcctcgtc catcgcgatg
     4021 tcgcagatga agcccaggtt ctcgcgcaga tgcagccaca ggatccccgc gcgccgatcg
     4081 tcatcgtcgc ggacggtcca gaaccggttc ccaggcgtct gcagtccctc gggcagcagt
     4141 tcgagcatct gctcggcggt cgtccggcgc gcggcctcga tcgtggggtc ggcaccggac
     4201 tcgaagcgct cctgcgcata ctcctcgtcc gacgcatcgc ggaaggcggc gtagtccgca
     4261 tcgtccatcg gatgcaggcg gatgccggcc ggcgcgggca cctctgcgac ctcgacgcgc
     4321 atcttcgtcg cgacgcggcg cgcatccgcg gattcggcga gcgcggtcat gaaggcgtcg
     4381 ccgctgaagg agctgaagtg ccagatgccc gtccagccgg cctcggccgc cagtccgtcc
     4441 agaagcatcc agaccgcagc agggtcctgt cccgtggtgt gcagatcggc gagcaccacc
     4501 tcatcctcgc gagggatagc ccacacggcc gcactgccgt catcggtaca ccagatctcc
     4561 ccggccgccg actcgggcac ggggacgtct tgccgttcgg ctgcggcctt cgctctgtac
     4621 gcacgcccta cggccgctgc gagcagctcg cctcgctgcc ggggctcggt gatctcgacc
     4681 cagctcacca gaagcggtcc ttcggctcgg cgggcttctt gtcggcgcca ccccagcgat
     4741 tcgcctcggc tttcgcggcg cccgctgcgg catcggcttc gcgcagcgcc tcctcggcgt
     4801 cgaccgcggc gggccgctcg ggctcctccg ctcgattctt ctccatgacc acactctagg
     4861 caccagaggc gaccggtggg cgcgaagggc gtcagtcctt cgcgtgctgc tgagtccacg
     4921 cctcgtcgcc gggggcgatc tcgcgcgcca tgtccctcag gaagcggatc accacctcgc
     4981 gctcggcgga ggtgaggcgg gccgccgcgt agaagcgctt cgcctgttgc ctgcccacgg
     5041 tctccatggc ggcctgatgc gtctgcgggg tgatcgagat cgccagcgcc cgccggtcgg
     5101 tggggtgagg cgcccgcacg atgtgcccgc cgcgctccag cctgtcgagg agcttggtcg
     5161 tcgaggccga cgatatgcgc agatgatggg cgatgccgcc gggggtcgcg aggatcccgc
     5221 gatgggcgca cacgatcaga tagtgcaggg ctcgcatgtc ggtgtcattg agccgcatgt
     5281 agcgacgcga ggcctcggag agctgctcct ccgcctcacg gagttccccc agcgacgaca
     5341 tcagctcggt gatctgccgc acatcggccg gcggcatgcc ctcacgatcg accagcacct
     5401 gctcgggatc gctgtagtcg acgtcgtaga cgtcggcgtc ggccaggccg tcaggcgtct
     5461 gcaccgaccg ccggccggcg accgggatgc cccctgcggc cgattcctct cgcatgcatc
     5521 gcattctagc cgttcctcta ttcacgccga gttgttttct agcttggcta gcaatatcct
     5581 cttccatgaa tcctgcgaag aagggcccgt catgtcgcac agcaccgaga agacgcaccg
     5641 cacctggcca cgagtcctga tctcgtcggc gctcgtcctg ctgtggctgg tgggagccgg
     5701 cctcggcgga ccgctgttcg ggaagatcga cgaggtctcc tccaacgatg cgacggcata
     5761 cctgccggat tcggcggcgg ccaccgaggt gcagaggtcg ctgggcgact tcctcggctc
     5821 cgactcgatc ccggccgtgg tggtcttctc gtccgacgcg aagatcgacg acgccctcaa
     5881 ggacgagctc gccgccaggc tcgaggatgc gaccgccgcc gaaggcgtga aggagggctc
     5941 ctcccccgtc ctcgtctcgg acgacgggct ggccgcgcag gcgttcgtgc cgatcgacgc
     6001 ggatgccgag gtcggggagg tcaccgccgc gctgtcggca gatctgcgca agagcgtgcc
     6061 ggacggcgtg accgtgcaca tcacgggacc ggccggcttc accgcggatc tgctcgccgg
     6121 gttctccggc atcgacggca tcctgctcct ggtggcgctg ctggcggtgc tcgtgatcct
     6181 cgtgatcgtg taccgctccc tcctgcttcc cctgatcgtg ctgtgcacca gcctgttcgc
     6241 cctgtgcgtg gcactgctgg tggtgtggtg ggcggccaag ctcgggatcc tgctgctgag
     6301 cggccagacc cagggcatcc tgttcatcct ggtgatcggc gccgcgacgg actactcgct
     6361 gctgctggtc gcacggttcc gcgaggagct gcacaccaca cagaaccgct gggccgcagt
     6421 gcgcgcagcg tggaagggcg ccttcgagcc gatcctggcc tccgggggca cggtgatcgc
     6481 gggtctgctg tgcctgctgc tgagcgatct gaagtccaac agcacgctgg gcccggtggc
     6541 ggcgatcggg atcgtgttcg cgatgctcgc cgccctcacc ctgctgcccg cactgctggg
     6601 cctcgccggg cgggcggcgt tctggccgcg ccgacccgcc ttcgaacctg gtctcacgcc
     6661 gcaggatgac ccgctgcag
//
LOCUS       DATMSX010000198         9904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_21469,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000198 DATMSX010000000
VERSION     DATMSX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21469"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VN041_06130"
     CDS             complement(<1..311)
                     /locus_tag="VN041_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28659.1"
                     /translation="MGVLDEGERVTAADAATWRGWLEANHATAPGVWLLNVRGSAADG
                     IDYEDAVRHALCFGWIDGPVRTFAENSVGQWFSPRRKGSGWAATNKARLAELEAARLL
                     AP"
     gene            396..1445
                     /locus_tag="VN041_06135"
     CDS             396..1445
                     /locus_tag="VN041_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU28660.1"
                     /translation="MAKMSDENADAAGRPTMKDVAARAGVGLSTVSRVVAGKGGVSRA
                     KSKAVERAIAELGYSRNDFAHTLRTGSARTIGVVVTRISDPFYSELVFTIEQRAQERD
                     LLVLVASGGDDPIEGERVLRRMLSRRLDGLIVVAHEEVDVSFLAAEKAAGTKIVFVDR
                     PPQGLSADLVIVDNEHGAATAVAHLVQAGHRSIACLAHTSGRYTSERRQAGFRQGIRD
                     AGLPARPELIVAVDDDVDACVEALSELASLDDPPTALFTTNSRTTKAVLKALRLLDAH
                     PALVGFDDFDLAELMSPPVTTVAQDPWAIGDAAADLLFDRIAGLVGPDRRIVLGTRMV
                     ERGSGETPPGAVTGL"
     gene            1535..2824
                     /locus_tag="VN041_06140"
     CDS             1535..2824
                     /locus_tag="VN041_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28661.1"
                     /translation="MRKALAAVAGLGVAALVLAGCSGSPGDGGSKGGSDDASQVEVFT
                     WWASGSEKTGLDALVKVFGEQFPKTKFVNAAVAGGAGSNAKNALASRLKADNPPDSFQ
                     AHAGAELTDYIEANKIEDLSSFYKDNGLNDVFPKTLLDRLTVDGKIYSVPSNIHRANV
                     VWVNNAVLTKAGIDPKTAPADIDAWLADMKKLKDAGVETPLSVATDWTQVQLFENVLL
                     ADLGADGYTGLWDGKTKFDDAKVTKAIKDYTSLLSYANTDGTGQDWPVATDMVIDGTA
                     AYNVMGDWALAEFDAKNKTYGTDYTTWPTPGTDGTFDFLADSFTLPVGAPHAGGAKNW
                     LKTISSADGQKAFNLAKGSIPARTDAVATDYPEYQQGAMASFAKDTVVSSLAHGAAVK
                     VSWLNDITSAIGQFVSTKDGKALQDALVQAQTKGLGK"
     gene            2895..3785
                     /locus_tag="VN041_06145"
     CDS             2895..3785
                     /locus_tag="VN041_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28662.1"
                     /translation="MRKGIRNWGPPLLLISPTIVLLGIFVYVMIGQNFYTAMTRDSAL
                     SASEFVGLENFTDLLTEPRFLHSLANLGIFTVFFMVGTMLFGFLWAWMLDKGVTAEGV
                     FRSVYLFPMAVSFVASGVVWRWLLSSAQGDRASGLNRLLESIGLGGLQNDWYNQPLWG
                     MAAMAMPAIWQLAGYVMALFLSGFRGIPEELREAARMDGASEWKLYRHVIFPQLSPVA
                     LSALVIVAHMSLKVFDLIMAVTGPVYITEVPATYMWTALTGGEYGKASAIATVLLLLV
                     CIFIVPYLISTARAERRARK"
     gene            3782..4699
                     /locus_tag="VN041_06150"
     CDS             3782..4699
                     /locus_tag="VN041_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28663.1"
                     /translation="MTVATGIERRATTAGSIPTAHRTRARIGRTTKYVLLLLFIVIVL
                     MPVYVLVVTSLKPPSDVSAVTSWAWPSRWPWQADGDGSTGFGNWVTAWDALAAPLGRT
                     FLLAIPAALVSAMLGSMNGFVLSRWKFPYANVVFTLILFGMFIPYQAIMVPLVQMKTS
                     LGLPSSIVTLLFVHIIYGLPICTLIFRNYYEGVPEELMEASRMDGAGMLGSYWRIILP
                     LSLPGFVVTIIWQFTSAWNDYLFALFLSDANQGPVSLSLVNLSQGAQLSNYGASMAGA
                     LIASIPTLLVYILLGKYFVGGLMSGSVKS"
     gene            4848..6173
                     /locus_tag="VN041_06155"
     CDS             4848..6173
                     /locus_tag="VN041_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28664.1"
                     /translation="MRKLKRVVVIATVGAVAAAALVGCGPKTGSEGDAKGVVKMVTWP
                     GPEGDAMAKVVKAYNAGQGKKDGITVDNVLISREDTFNKEVALMTAKDSSMDVYFTAT
                     YNVGQFSASLDALDGIDTSAYFPVAVDGLKYNDKLYALPLDVSNHFLYYRTDLLDALA
                     SDPAQTAKYAEISKKVVGEALVPKKPEDWSMDDFKATVAYFSQAENPASPTKYGTILQ
                     AKDLLYNTMIWNDVLWGYGGSWTDDSGKAAIDSDAGRKAVQLYADIYKNGWTSPDSAQ
                     AEFPETQAALQSGQVAVALQWSAAYADLADAAKSPKIADKIAIAPVPGGASHVHALAV
                     AINKYGKNKAAARKWLEYLATPEAMDAYAKAGGIPAMPDVLKQNTDLNAAFPVVIDAV
                     SKGYAEPVFPKTFEAYKELADALSPAWVGQKPADEAVKQANAKLQDLLP"
     gene            6247..7128
                     /locus_tag="VN041_06160"
     CDS             6247..7128
                     /locus_tag="VN041_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28665.1"
                     /translation="MQSQAMRQRRWTAALLAAPLLIFAVCFVAYPLATGALTAFQDKT
                     VYSPEETFSGLKNFGALFRDGDFVQAVVFTVLYTVVVVAVEVVLGFGLALLMHRVMPG
                     KKLWFTLILLPIMIAPAMLGVMFRLLLNGDIGAVPAILEHLGVSVSLFSPGSVIPLLI
                     VLDILQWTPFTFLIIYAGLQSFPLELSEAAAVDGAGYWRTLRSVVLPVIAPVFIAAMF
                     LRMIDALRTFDVIYVLTAGGPGGMTTTVSIYIYKTAFVSGDFGKAAAAALVMLVCVLP
                     LVPVIVKRITQSGGRNA"
     gene            7121..7972
                     /locus_tag="VN041_06165"
     CDS             7121..7972
                     /locus_tag="VN041_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28666.1"
                     /translation="MPEQTLRRHRRHTTGAVIAAVAAAVIALLLNAPFLNSLLVSFKT
                     EGDIAGDPLGFSSGPTLDHYLNVLYQAGYDFPRFFLNSILISLGAVLLVLIISVPASY
                     AIIRLRFGGDAVVNAASGLRLLPAVFFIVPFFVMFNLVGLTDTVAGLVFANTFLNVPL
                     ALILLTGAIRELPIEVEEAAAVDGAGILATLWRIVIPMLAPTLVAVCVLVFIFSWDDY
                     LFALVLSTSEATPVTLGAANFITSTGIRWGDISAAAVLSVIPPVLFAVFAQRYLVSGL
                     SSGAVKG"
     gene            7976..8677
                     /locus_tag="VN041_06170"
     CDS             7976..8677
                     /locus_tag="VN041_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28667.1"
                     /translation="MHKYEVLATDLRSLVATMSAHEPLPSERDLMVRYGVSRATVRQA
                     IGRLVDEGRLYNVHGSGTFVGSNDLFTKSPKLTSFTEDMRSRGFEPSSRVLSVSSVRA
                     SAATVAMLQLDADEKCTQIRRLRLADGHPMALEEVTIPARFLEVEDFDMTASLYEQFA
                     ARDLDVYRAEQEVQAVSADSATADLLGLAAGGACLHVTRVGSTRRGLLVEHGQTWYRG
                     DRYSFQFAVTREHEK"
     gene            8689..9363
                     /locus_tag="VN041_06175"
     CDS             8689..9363
                     /locus_tag="VN041_06175"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HWU28668.1"
                     /translation="MLTALAGRLVVSCQAYPGEPLRTPGSMTRMALSAVAGGAAGIRA
                     QGIEDIAMIDDCVDVPLIGLWKIGTEGVFITPTLEAALACARAGADIVAVDGTRRPRP
                     DGLSLADVVAEVHEQTDALVMADVGSLEDARHAEDAGADVISTTLAGYTPERERTRGP
                     DLELLTQIVRSTALPVFAEGRIHTPQQAADAMAAGATAVVVGTAITHPATITTWFAAA
                     VNRNAN"
     gene            complement(9397..9564)
                     /locus_tag="VN041_06180"
     CDS             complement(9397..9564)
                     /locus_tag="VN041_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28669.1"
                     /translation="MTQQDMLFLVMLVIGLTGLTVLTLQLLKRPKRDQKGEWYEGPWD
                     DDDRPRGPHNG"
     gene            9684..>9904
                     /locus_tag="VN041_06185"
     CDS             9684..>9904
                     /locus_tag="VN041_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase"
                     /protein_id="HWU28670.1"
                     /translation="MTILTPALDHAALRAIPGLMLPGDPGYDEARTPWNLAADQHPAA
                     VAEPTTIADAARIVGAVAALGLRIAPQASG"
BASE COUNT         1551 a         3482 c         3242 g         1629 t
ORIGIN      
        1 ggcgccagca gccgggcagc ctccagctcg gcgaggcggg ccttgttggt cgcggcccag
       61 ccgctgcctt tgcggcgcgg cgagaaccac tggccgacgg agttctcggc gaacgtgcgc
      121 accgggccgt cgatccaccc gaagcagagg gcatgacgca ccgcgtcctc gtagtcgatg
      181 ccgtccgccg cggacccgcg cacgttcagc agccacacac cgggcgctgt ggcgtggttg
      241 gcctccagcc agccgcgcca ggtcgcggca tccgccgccg tcacccgctc gccctcgtcg
      301 agaaccccca tccgcacctc cgtgtcgccg tgggcccagc ctagggcgcg ccgtcgacat
      361 ggacttggca acgttttcat gaaatcgttc tgggtatggc gaagatgagc gacgagaacg
      421 cggatgccgc gggtcgcccc accatgaagg acgtcgcggc gcgcgccggc gtcgggctgt
      481 cgaccgtctc gcgcgtcgtc gcgggcaagg gcggggtcag ccgcgccaag tccaaggcgg
      541 tcgagcgtgc gatcgccgaa ctcgggtaca gccgcaacga cttcgcgcac acgctgcgca
      601 ccgggtcggc gcggaccatc ggcgtcgtcg tgacgcgcat ctccgacccg ttctactccg
      661 aactggtctt cacgatcgag cagcgcgcac aggaacgcga cctgctggtg ctggtcgcct
      721 ccgggggcga cgacccgatc gagggggagc gggtgctgcg gaggatgctg agccgtcggc
      781 tggacggcct gatcgtcgtc gcccacgagg aggtcgacgt gtcgttcctc gcggcggaga
      841 aggccgcggg cacgaagatc gtgttcgtcg accggccacc gcaaggactg agcgccgatc
      901 tcgtcatcgt ggacaacgag cacggtgccg ccaccgccgt ggcccatctc gtgcaggccg
      961 ggcatcgcag catcgcctgt ctcgcgcaca cctccggccg gtacacctcc gagcgcaggc
     1021 aggccgggtt ccggcagggc atccgggatg ccggactgcc ggcgcgtccc gagctcatcg
     1081 tcgccgtcga cgacgacgtc gacgcgtgcg tcgaggcgct gagcgagctc gcctctctgg
     1141 acgacccgcc cacagcgctg ttcaccacca acagccgcac caccaaggcg gtcctcaagg
     1201 cgctgcggct gctcgacgcg catccggccc tggtcggctt cgacgacttc gacctggccg
     1261 agctgatgag cccgccggtg acgaccgtcg cgcaggaccc gtgggcgata ggcgatgccg
     1321 cagccgacct gctgttcgac cggatagccg gtctcgtcgg cccggatcgc cgcatcgtgc
     1381 tgggcacgcg gatggtcgag cgcggttcgg gggagacgcc gcccggggct gttaccgggt
     1441 tgtgaccccg accccgttgc gtttggcaac gttttcagat tcgatgacag aaggcccgcg
     1501 cacagcaggc cgtcttcatc cgatggagga tcacatgcgc aaagcgctcg cagcagtagc
     1561 gggtctcggt gtcgcggctc tcgtcctggc aggctgctcc ggcagccccg gcgacggcgg
     1621 cagcaagggc ggctccgacg atgccagcca ggtcgaggtg ttcacctggt gggcatccgg
     1681 ctccgagaag acgggcctgg acgcgctcgt caaggtcttc ggcgagcagt tcccgaagac
     1741 gaagttcgtc aacgcggccg tcgccggcgg tgcgggatcg aacgcgaaga acgcgctcgc
     1801 ctcgcgcctg aaggccgaca acccgccgga ctcgttccag gcgcacgccg gtgccgagct
     1861 caccgactac atcgaggcga acaagatcga ggacctcagc tccttctaca aggacaacgg
     1921 cctcaacgac gtcttcccga agacgctgct cgaccgcctc accgtcgacg gcaagatcta
     1981 ctcggtgccc agcaacatcc accgcgccaa cgtcgtgtgg gtcaacaacg cggtgctgac
     2041 caaggccggc atcgacccga agacggcgcc cgccgacatc gacgcgtggc tcgccgacat
     2101 gaagaagctc aaggacgcgg gcgtcgagac cccgctctcg gtggcgaccg actggaccca
     2161 ggtgcagctg ttcgagaacg tgctgctcgc cgacctcgga gccgacggct acacgggcct
     2221 gtgggacggc aagaccaagt tcgacgacgc caaggtgacc aaggcgatca aggactacac
     2281 ctcgctgctg tcgtacgcga acaccgacgg caccggccag gactggccgg tcgcgaccga
     2341 catggtcatc gacggcaccg ccgcgtacaa cgtgatgggc gactgggctc tggccgagtt
     2401 cgacgccaag aacaagacct acggcaccga ctacaccacc tggccgaccc cgggcaccga
     2461 cggcacgttc gacttcctcg ccgactcctt cacccttccg gtcggtgctc cgcacgccgg
     2521 cggcgcgaag aactggctca agacgatcag ctccgccgac gggcagaagg cgttcaacct
     2581 cgccaagggc tcgatcccgg cgcgcaccga cgcggtcgcg accgactacc ccgagtacca
     2641 gcagggcgcg atggcctcgt tcgccaagga caccgtggtc agctcgctgg cccacggcgc
     2701 ggccgtgaag gtctcctggc tgaacgacat cacctccgcc atcgggcagt tcgtctcgac
     2761 caaggacggc aaggctctgc aggacgctct cgtccaggcg cagaccaagg gcctcggcaa
     2821 gtaacatcca tcctccgagc ggggcggcgc accggcgccg ccccgctcgc atccccgtct
     2881 gaaggagggc gccgatgcgc aagggcatcc gcaactgggg gccgccgctg ctgctgatct
     2941 cgccgacgat cgtgctgctg ggcatcttcg tctacgtcat gatcggccag aacttctaca
     3001 ccgccatgac cagggactcc gccctgtcgg catccgagtt cgtgggcctt gagaacttca
     3061 ccgacctgct caccgaaccg cggttcctgc actcgctggc caacctcggc atcttcaccg
     3121 tgttcttcat ggtcgggacc atgctcttcg ggttcctctg ggcgtggatg ctggacaagg
     3181 gcgtcacggc ggagggcgtg ttccgctcgg tctacctctt cccgatggcc gtctcgttcg
     3241 tggcatccgg cgtggtctgg cggtggctgc tcagctcagc gcagggcgac cgcgcctcgg
     3301 gcctgaaccg cctgctcgag tccatcgggc tcggcggcct gcagaacgac tggtacaacc
     3361 agccgctgtg gggcatggcc gccatggcca tgcccgccat ctggcagctc gccggctacg
     3421 tgatggcact gttcctctcc gggttccgcg gcatccccga ggagctgcgc gaggccgccc
     3481 gcatggacgg cgccagtgag tggaagctct accgtcacgt gatcttcccg cagttgagcc
     3541 cggtggccct gtcggccctc gtgatcgtcg cgcacatgtc gctgaaggtc ttcgacctga
     3601 tcatggccgt caccggtccg gtgtacatca ccgaggtccc ggcgacctac atgtggaccg
     3661 cgctgaccgg cggcgagtac ggcaaggcat ccgccatcgc gaccgtcctg ctgctgctgg
     3721 tgtgcatctt catcgtgccg tacctgatct ccacggcccg cgccgagagg agggcacgca
     3781 agtgaccgtc gcaaccggaa tcgagcgccg cgccaccacc gccggcagca tcccgaccgc
     3841 gcaccgaacc cgcgcgcgca tcggccgcac caccaagtac gtgctgctcc tgctgttcat
     3901 cgtcatcgtg ctgatgccgg tgtatgtgct ggtggtcacc agtctgaagc cgccgtccga
     3961 cgtcagcgcc gtcacctcgt gggcgtggcc gtcgcggtgg ccgtggcagg ccgacggaga
     4021 cggcagcacc ggcttcggca actgggtcac cgcctgggat gcgctggctg cgccgctcgg
     4081 ccgcaccttc ctgctggcga tccccgccgc cctcgtctcg gccatgctcg gctcgatgaa
     4141 cggcttcgtg ctctcgcgct ggaagttccc ctacgccaac gtggtgttca cgctgatcct
     4201 gttcggcatg ttcatcccgt accaggcgat catggtgccg ctggtgcaga tgaagacctc
     4261 gctggggctg ccgtcgagca tcgtcacgct gctgttcgtg cacatcatct acggtctgcc
     4321 gatctgcacg ctgatcttcc gcaactacta cgagggcgtg cccgaggagc tcatggaggc
     4381 gtcccgcatg gacggcgcag gcatgctcgg ctcgtactgg cgcatcatcc tgccgctgtc
     4441 gctgccgggg ttcgtcgtga cgatcatctg gcagttcacg tcggcgtgga acgactacct
     4501 gttcgcactg ttcctctcgg atgccaatca ggggcccgtc tcgctgtccc tggtcaacct
     4561 gtcgcagggc gctcagctgt cgaactacgg cgcgtcgatg gccggtgcgc tgatcgcgtc
     4621 gatcccgacg ctgctcgtgt acatcctgct cggcaagtac ttcgtcggcg gactgatgag
     4681 cggatccgtc aagtcctgag ctgtgaccgc gcaggacgcg aatggggagt ctggtatgta
     4741 ccagactccc cattcttttg ctttcctcct gcgtgctctt gaccttctcg cggaactctg
     4801 tcaaccttgc ggacaaggtg gttcgtacca gtcatcaagg aggatgcatg cgaaagctca
     4861 agagagtcgt cgtgatcgcg accgtcggcg ccgtggcagc cgccgcgctc gtgggctgcg
     4921 gcccgaagac gggcagcgag ggcgacgcca agggagtcgt gaagatggtg acctggcccg
     4981 gccctgaagg cgacgccatg gcgaaggtgg tgaaggccta caacgccggt cagggcaaga
     5041 aggacgggat caccgtcgac aacgtcctga tctcgcggga ggacaccttc aacaaggagg
     5101 tcgcgctgat gacggcgaag gactccagca tggacgtgta cttcacggcc acctacaacg
     5161 tgggtcagtt ctcggcatcg ctcgacgcgc tcgacgggat cgacacctcg gcgtacttcc
     5221 cggtcgccgt cgacgggctc aagtacaacg acaagctgta tgcgctgccg ctggacgtgt
     5281 ccaaccactt cctgtactac cgcacagatc tgctcgacgc gctcgcgagc gaccccgcgc
     5341 agaccgcgaa gtacgccgag atctcgaaga aggtcgtcgg cgaggcgctc gtccccaaga
     5401 agcccgagga ctggtcgatg gacgacttca aggccacggt cgcgtacttc tcgcaggcgg
     5461 agaacccggc atccccgacg aagtacggca cgatcctgca ggcgaaggac ctgctgtaca
     5521 acaccatgat ctggaacgac gtgctctggg gctacggcgg atcctggacc gatgactcgg
     5581 gcaaggccgc gatcgactcg gatgccggac gcaaggccgt gcagctgtac gccgacatct
     5641 acaagaacgg ctggacctcg cccgacagcg cacaggccga gttccccgag acgcaggcgg
     5701 ccctgcagtc cggacaggtc gccgtggcgc tgcagtggtc ggccgcgtac gccgatctgg
     5761 ccgatgccgc caagtcgccc aagatcgccg acaagatcgc gatcgctccc gtgccgggtg
     5821 gggcatcgca cgtccacgcc ctcgccgtcg cgatcaacaa gtacggcaag aacaaggccg
     5881 cagcgcgcaa gtggctcgag tacctcgcga ccccggaggc gatggatgcc tatgccaagg
     5941 ccggcggcat cccggcgatg cccgatgtgc tcaagcagaa caccgacctc aacgccgcgt
     6001 tcccggtggt catcgacgcc gtcagcaagg gctatgcgga gccggtcttc cccaagacct
     6061 tcgaggcgta caaggagctg gccgacgcgc tcagcccggc gtgggtcggg cagaagccgg
     6121 ccgacgaggc ggtgaagcag gccaacgcga agctgcagga cctgctgccc tgaccgtgcg
     6181 gcgggggcgc gcgagcgctc ccgccggcac cgcccccgtc cacccgccag ctccgaggag
     6241 acctgaatgc aatctcaggc catgcgccag cgtcgatgga ccgcggcgct gctcgccgca
     6301 ccgctgctga tcttcgccgt gtgcttcgtc gcctaccccc tggcgaccgg cgcactgacc
     6361 gccttccagg acaagaccgt gtactccccg gaggagacgt tcagcggcct gaagaacttc
     6421 ggggcactgt tccgcgacgg cgacttcgtg caggcggtgg tcttcaccgt cctctacacg
     6481 gtcgtcgtgg tcgccgtcga ggtcgtgctg ggattcgggc tcgcgctgct catgcaccgc
     6541 gtgatgccgg gcaagaagct gtggttcacg ctcatcctgc tgcccatcat gatcgccccc
     6601 gcgatgctcg gcgtgatgtt ccgtctgctg ctgaacggcg acatcggcgc cgtcccggcc
     6661 atcctggagc acctcggggt cagcgtgagc ctgttctcgc ccggctccgt gatcccgctg
     6721 ctcatcgtcc tcgacatcct gcagtggacg cccttcacct tcctcatcat ctacgcgggc
     6781 ctgcagtcgt tcccgctgga gctcagcgag gcggccgcgg tcgacggggc cgggtactgg
     6841 cgcacgctgc gcagcgtggt gctccccgtc atcgcccccg tgttcatcgc cgcgatgttc
     6901 ctccggatga tcgacgcgct ccgcacgttc gacgtcatct acgtgctgac ggcgggcgga
     6961 cccggcggca tgacgacgac ggtgagcatc tacatctaca agaccgcctt cgtgtcgggc
     7021 gacttcggca aggccgccgc cgctgccctc gtcatgctgg tctgcgtgct tccgctggtc
     7081 cccgtcatcg tcaagcgcat cacccagtcg ggaggacgca atgcctgagc agaccctccg
     7141 ccgtcatcgg cggcacacga cgggcgctgt catcgccgca gtcgcggccg ccgtcatcgc
     7201 gctcctcctc aacgccccgt tcctgaactc cctcctcgtc agcttcaaga ccgagggaga
     7261 catcgccgga gatccgctcg ggttctcgtc cgggcccacg ctcgaccact acctgaacgt
     7321 gctgtatcag gccggctacg acttcccgcg cttcttcctc aactccatcc tgatctcgct
     7381 gggcgcggtc cttctcgtgc tgatcatcag cgtgcccgcc tcctatgcga tcatccggct
     7441 gcgattcggt ggggacgcgg tcgtcaacgc ggcatccgga ctccggcttc tgcccgcggt
     7501 gttcttcatc gtcccgttct tcgtgatgtt caacctggtc gggctcaccg acaccgtggc
     7561 agggctcgtc ttcgcgaaca cgttcctgaa cgtgccgctg gcgctgatcc tgctcaccgg
     7621 cgccatccgc gagctgccca tcgaggtcga ggaggccgcc gccgtcgacg gtgcaggcat
     7681 cctcgcgacg ctgtggcgga tcgtgatccc catgctggcg ccgacgctcg tcgcggtgtg
     7741 cgtgctggtc ttcatcttca gctgggacga ctacctgttc gccctggtgc tgtcgacctc
     7801 cgaggcgacg cccgtcacgc tgggggccgc gaacttcatc accagcacgg ggatccgctg
     7861 gggcgacatc tccgcagcag ccgtgctgtc cgtgatcccg cctgtgctct tcgccgtgtt
     7921 cgcgcagcgc taccttgtga gtggactttc gtccggagcg gtgaagggct gagggatgca
     7981 taagtacgag gtgctggcca ccgacctgag gtcgctcgtg gcgacgatgt cggcgcacga
     8041 gccgcttccc tccgaacgag atctcatggt gcgctacgga gtcagccgtg cgacggtgcg
     8101 ccaggcgatc ggccggctcg tcgacgaggg gcgtctctac aacgtgcacg gctccggcac
     8161 cttcgtgggc tcgaacgacc tgttcaccaa gtcgccgaag ctcacgtcct tcacggagga
     8221 catgcgcagc cgcgggttcg agccgtcgtc gcgggtgctc tcggtgagct cggtgcgcgc
     8281 gtcggccgcg accgtggcga tgctgcagct ggacgcggat gagaagtgca cgcagatccg
     8341 ccgtctgcgt ctggctgacg ggcatccgat ggcgctcgag gaggtcacga tcccggcgcg
     8401 gttcctcgag gtggaggact tcgacatgac cgcctccctc tatgagcagt tcgccgcacg
     8461 cgacctcgac gtctaccggg cggagcagga ggtgcaggcg gtctcggccg attccgcgac
     8521 cgccgacctg ctgggtctcg cggccggagg cgcctgcctg cacgtcaccc gggtcggctc
     8581 gacccgtcgc ggactgctcg tcgagcacgg ccagacgtgg taccgcggcg atcggtactc
     8641 gttccagttc gccgtgacca gggagcacga gaagtgaccc cggagcagat gctgaccgca
     8701 ctggccggac gcctggtcgt ctcgtgccag gcgtaccccg gcgaaccgct gcgcacaccc
     8761 ggctccatga cccggatggc actgtcggcg gtggcgggcg gtgcggccgg catccgcgcc
     8821 caggggatcg aggacatcgc catgatcgac gactgcgtcg acgtgcccct catcggtctc
     8881 tggaagatcg ggaccgaagg ggtcttcatc actcccaccc tggaggccgc cctggcgtgc
     8941 gccagggcgg gcgccgacat cgtcgccgtg gacggcacgc gccgcccgcg accggacggc
     9001 ctgtcgctcg ccgacgtggt cgccgaggtg cacgagcaga cggatgccct ggtgatggcc
     9061 gacgtgggca gcctcgaaga cgcgcgtcac gccgaggacg ccggagcgga cgtcatcagc
     9121 accaccctcg ccgggtacac tcccgaacgg gagcgcacgc gcggccccga cctcgagctg
     9181 ctcacgcaga tcgtccgctc caccgcgctc cccgtattcg cggaagggcg catccacacc
     9241 cctcagcagg cggcggatgc catggccgcc ggcgcgacgg ccgtggtcgt gggcaccgcg
     9301 atcacgcatc ccgcgaccat cacgacctgg ttcgccgcgg ccgtgaatcg caacgcgaac
     9361 tgagctccgc cgggatgctc gcctgtcgcg acgggctcag ccgttgtggg ggcctcgcgg
     9421 acgatcgtcg tcgtcccagg ggccctcgta ccactcgccc ttctggtcgc gcttggggcg
     9481 cttgagcagc tgcagggtca gcacggtcag gccggtcagg ccgatgacga gcatgaccag
     9541 gaagagcatg tcctgctgag tcatcggcgt cctctctcga tgggaccagc ctaggcggtg
     9601 cagttcggcg gatgctgcgt ccatcacgaa tctccgtgtt ctccgggatg tccggagtgc
     9661 atccggcggc gcacactcga gccatgacca tcctcacgcc tgccctggac cacgccgcgc
     9721 tgcgtgcgat ccccgggctg atgctgcccg gcgaccccgg atacgacgag gctcgcacac
     9781 cctggaacct ggctgcggac cagcatccgg cagccgtcgc ggaaccgacg acgatcgccg
     9841 acgcggcgcg catcgtgggc gccgtcgcgg cactcggact gcggatcgcc ccgcaggcga
     9901 gcgg
//
LOCUS       DATMSX010000199         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135311,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000199 DATMSX010000000
VERSION     DATMSX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135311"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VN041_06190"
     CDS             <1..1045
                     /locus_tag="VN041_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28671.1"
                     /translation="YEIARFVQRCAMSDISDTDAAASVRDDLQPLMDQYGSCEGRTSA
                     LPYSVMAASVIYNKEIFRAQGLEVPQTWDELIALCEQLKDSGIDPFYATFKDDWTVGQ
                     GWYDYSIGGSVDTIEFFDAMAVEGAAVGPDSEVSFEKDFAEPMEQMMQLADDYTNEDA
                     ASRGYGDGNLAFSKGEAAMYMQGPWAFSEIAKTAPDLELGTFPLPMTDDPADLGVRVN
                     MDLGAMIPEGSRHQEAARDFLEYLYLPENIEEYNASQLGFTPTKDAPAPDDPRIEGMI
                     DYYEDGQIYQGPSVLVPKTIPVMNYAQAMVLGASTTSTLRTMDADWARIAFRAPIPTT
                     GDAAAGETEESAP"
     gene            1105..1998
                     /locus_tag="VN041_06195"
     CDS             1105..1998
                     /locus_tag="VN041_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28672.1"
                     /translation="MVTGQGRGLRRHTRRVEPIYYLFLLPTLVLFTLAITLPAVMGIF
                     FSFTDSIGIGEWSFNGLTNYIAMFSDPAILQSYLFTFGFSIATVIVVNVIAFLLAVGL
                     TSRIRFKTGLRTIFVIPMVISGIIIAYVFNFLFSNSIPAAGAATGIPWLETSLLANPD
                     LAWVAIVIVTAWQAVPGALLIYIAGLLSVPNEVYEAASIDGASKTQQLTRITIPLVAG
                     YVVINVILGLEGFLNAYDIIVGLTNGGPGTSTRSIAMTIIAGFNGGDYAYQMANATIF
                     FIVAVLISLLQLSLTRGRNAL"
     gene            1998..2876
                     /locus_tag="VN041_06200"
     CDS             1998..2876
                     /locus_tag="VN041_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU28673.1"
                     /translation="MSTQTMTTVTADGRRPRIRMERVNWSGTIILFLCAVTVLLPLYV
                     TISMAFKTTGQAVDGNAFSLPAPFSLDGFVEAWNLTKFPVGAAISLAVTAGTVIATIV
                     LAAFASYAIVRNWDRRLFRWSFFYLLAAMFIPFPVVALPQIQLTGRVGLDNPLGVIIL
                     ATMFQLSFSVLLFTAFLRSIPLELEESARMDGASTWQTFWRIIFPLLGPMSATVGIFA
                     FLYAWNDFMMPSLIISDPALQTLPVRQNLFQNQFSNNYNVSFASYLMAMAPAIVAYLF
                     TQRWVMEGVTQGAVKG"
     gene            2962..4638
                     /locus_tag="VN041_06205"
     CDS             2962..4638
                     /locus_tag="VN041_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HWU28674.1"
                     /translation="MTDALLVESRTDDAAAWWRQAAVYQIYPRSFADANGDGLGDIPG
                     ILSRVDYLAELGIDAVWLSPFYPSALADGGYDVDDYRNVDPRLGTLEDFDAMVEALHA
                     RGIRVVVDIVPNHTSNRHEWFQEALAAGRGSAARDRYIFREGTGPDGAEPPTDWVSVF
                     GGSAWERVEDGQWYFHNFAVEQPDLNWSSTEVREDFLTTLRFWSDRGVDGFRIDVAHM
                     LTKDLSEPLPSRAELALLPQDGSHPLIDRDDVHEIYAEWRKVFDSYDPPRTAVAEAWV
                     STPERRARYASAEGLGQAFNFDLLVADFDAEQFRRIIADNLAQATLSGSSTTWVLSNH
                     DVTRHATRYGLPPLNGRAVKQGTEWVAAGGPAEQLDRDRGRRRAHAATLLLLGLPGSA
                     YLYQGEELGLHEVAEITPEQRQDPAFFRGGEFDGLGRDGCRVPLPWSASGASFGFGAD
                     GAHLPQPAWFAEYAVDVEAADPASTLSLYREALRLRHELQADETLEWLETGRVDVLRF
                     ARPNGWQIVTTFGAEPFDLGEDAARVALSSLPLVGGAMPGEATAWIAPGV"
BASE COUNT          757 a         1617 c         1526 g          763 t
ORIGIN      
        1 ctacgagatc gcgcgcttcg tgcagcggtg cgccatgagc gacatctccg atacggatgc
       61 cgccgcatcc gtgcgcgacg acctgcagcc cctgatggat cagtacggct cgtgcgaggg
      121 ccgcacgagt gcgttgccgt actcggtgat ggcggcctct gtgatctaca acaaggagat
      181 cttccgggcg caggggctcg aggtgccgca gacgtgggac gagctgatcg ccctgtgcga
      241 gcagctgaag gattctggga tcgacccctt ctacgcgacg ttcaaggacg actggacggt
      301 cggtcagggc tggtacgact actcgatcgg tggctccgtc gacacgatcg agttcttcga
      361 cgccatggcc gtcgagggcg ccgcggtcgg ccccgactcc gaggtctcgt tcgagaagga
      421 cttcgccgag cccatggagc agatgatgca actcgcggac gactacacga atgaggatgc
      481 ggcgagccga ggctacggcg acggcaatct cgccttcagc aagggcgagg cggccatgta
      541 catgcagggg ccgtgggcgt tcagcgagat cgcgaagacc gcacccgacc tcgagctcgg
      601 aaccttcccg ctgccgatga ctgacgaccc ggccgacctc ggcgtgcgcg tgaacatgga
      661 cctcggcgcg atgatcccgg agggatcgcg gcaccaggag gccgcgcgcg acttcctgga
      721 gtacctgtac ctcccggaga acatcgagga gtacaacgca tcgcagctcg gcttcacccc
      781 gacgaaagat gcccccgcac cggacgatcc gcggatcgag gggatgatcg actactacga
      841 ggacgggcag atctaccagg ggccctcggt gctcgtgccc aagacgatcc cggtgatgaa
      901 ctacgcacag gcgatggtgc tgggagcctc gaccacctcg acgcttcgga ccatggacgc
      961 ggactgggct cgcatcgcct tccgtgcgcc gatccccacc acaggcgatg cggcagcagg
     1021 cgagacagag gagtccgcac cgtgagcacc actgcacccg cgaacatcac gcgaccgaac
     1081 agcccgacga ccgatacgac ctcgatcgtc accggacagg ggcgggggct ccgccggcac
     1141 acccggaggg tcgagccgat ctactacctg ttccttcttc cgacgctcgt gctcttcacc
     1201 ctcgcgatca cgctccccgc ggtgatgggc atcttcttca gcttcaccga ctcgatcggc
     1261 atcggggagt ggagcttcaa cgggttgacc aactacatcg cgatgttcag cgacccggcc
     1321 atcctgcaga gctacctctt caccttcggg ttctctatcg ccacggtgat cgtcgtgaac
     1381 gtcatcgcgt tcctgctcgc ggtgggtctg acctcccgca tccgcttcaa gactgggctg
     1441 cgcacgatct tcgtgatccc gatggtgatc tcgggcatca tcatcgcgta cgtcttcaac
     1501 ttcctcttct cgaactcgat ccccgccgcg ggcgcggcca ccggcatccc gtggctcgag
     1561 acgagcctgc tggcgaaccc ggatctcgcc tgggtggcga tcgtgatcgt gaccgcgtgg
     1621 caggccgtcc ccggtgcact gctgatctac atcgcggggc tgctctccgt gccgaacgag
     1681 gtctacgagg cggccagcat cgacggtgcg agcaagacgc agcagctcac ccgcatcacg
     1741 atcccgctcg tcgcggggta cgtcgtgatc aacgtgatcc tcggattgga gggcttcctg
     1801 aacgcctacg acatcatcgt cggcctcacc aacggcggtc ccggaacctc cacccgctcc
     1861 atcgcgatga ccatcatcgc cgggttcaac ggcggcgact acgcgtacca gatggccaac
     1921 gcgaccatct tcttcatcgt cgccgtcctc atctccctgc tgcagctctc gctgactcgc
     1981 ggaaggaacg ccctctgatg tcgacgcaga ccatgaccac cgtcaccgcc gacggccgcc
     2041 gaccccgcat caggatggag cgggtgaact ggtcgggcac gatcatcctg ttcctgtgcg
     2101 cggtcaccgt gctgctgcct ctgtacgtca cgatctcgat ggcgttcaag acgacaggtc
     2161 aggccgtgga cggcaatgcc ttctcactgc ccgcgccgtt cagcctggac ggattcgtcg
     2221 aggcgtggaa tctcacgaag ttcccggtcg gcgccgcgat ctccctggcg gtcaccgcgg
     2281 gaaccgtcat cgcgaccatc gtgctggccg cgttcgcctc ctacgcgatc gtgcgcaact
     2341 gggaccgtcg cctgttccgg tggtcgttct tctacctgct cgcggcgatg ttcatcccgt
     2401 tccccgtcgt cgcactgccg cagatccagc tcaccgggcg ggtcggactc gacaacccgc
     2461 tgggggtgat catcctcgcg acgatgttcc agctcagctt cagcgtgctg ctgttcaccg
     2521 cgttcctgcg gtccatccca ctggagctcg aggagagcgc ccgcatggac ggcgccagca
     2581 cgtggcagac gttctggcgg atcatcttcc cgctgctggg cccgatgagc gccacggtcg
     2641 gcatcttcgc gttcctctac gcgtggaacg acttcatgat gccgtcgctg atcatctccg
     2701 atccggcgct gcagacgctg cccgtgcggc agaacctgtt ccagaaccag ttcagcaaca
     2761 actacaacgt gtccttcgcg tcgtacctca tggcgatggc gccggccatc gtcgcctacc
     2821 tgttcacgca gcgctgggtc atggagggcg tgacgcaggg cgccgtcaag ggctgagcgc
     2881 aaaccgctcg gccggcggat gctgaacgca gagcacgaca gaaccgacga cagaccacga
     2941 agagaaggag cagtaccccg catgactgac gcgctactcg tcgagagccg taccgacgac
     3001 gcagccgcct ggtggcgcca ggcggccgtc taccagatct acccgcgcag cttcgccgac
     3061 gcgaacggtg acggcctcgg cgacatcccc ggcatcctgt cgagggtgga ctacctcgcc
     3121 gagctcggca tcgacgcggt ctggctgagc ccgttctacc cctctgcact cgccgacggc
     3181 ggctacgacg tcgacgatta ccggaacgtc gatccacggc tgggcacgct ggaggacttc
     3241 gacgcgatgg tggaggcgct gcacgcccgc ggcatccgcg tcgtggtgga catcgtgccg
     3301 aaccacacct cgaatcggca cgagtggttc caggaggcgc tcgccgccgg ccgggggtcg
     3361 gcggctcgcg accggtacat cttccgcgag ggcacgggac ccgacggcgc cgagccgccc
     3421 accgactggg tgtcggtctt cggcggctcc gcctgggagc gtgtcgagga cgggcagtgg
     3481 tacttccaca acttcgcggt cgagcagccc gacctgaact ggtcgagcac cgaggtgcgc
     3541 gaggacttcc tgacgacgct gcggttctgg tcggaccggg gcgtggacgg cttccgcatc
     3601 gacgtcgcgc acatgctgac caaggatctc agcgagccgc tgcccagccg cgcggagctc
     3661 gcgctgctgc cgcaggacgg cagccacccg ctgatcgacc gcgacgacgt gcacgagatc
     3721 tatgccgagt ggcggaaggt gttcgactcc tacgacccgc cccgaacggc cgtcgcggag
     3781 gcgtgggtgt cgacgccgga gcgtcgcgcc cggtacgcgt ccgcggaggg gctcggtcag
     3841 gcgttcaact tcgacctgct ggtcgcggac ttcgacgccg agcagttccg ccgcatcatc
     3901 gccgacaacc tcgcccaggc gaccctgagc ggctcgtcca ccacgtgggt gctgtcgaac
     3961 cacgacgtca cccggcacgc gacccggtac ggactgccgc cgctgaacgg ccgcgcggtc
     4021 aagcagggca cggagtgggt cgccgcgggc ggtcccgcgg agcagctcga ccgcgatcga
     4081 ggccggcggc gtgcgcacgc cgccacgctg ctgctgctcg ggctgcccgg cagcgcctac
     4141 ctgtatcagg gcgaggagct cggactgcac gaggtcgccg agatcacgcc cgagcagcgg
     4201 caggacccgg cgttcttccg gggcggcgaa ttcgacgggc taggcaggga cggctgccgc
     4261 gtgccgctgc cgtggtcggc gagcggagcc tccttcgggt tcggagcgga cggcgcccac
     4321 ctgccgcagc ccgcgtggtt cgcggagtac gccgtcgatg tcgaggccgc tgacccggca
     4381 tcgacgctgt ccctgtaccg ggaggcgctc cggctgcggc acgagctgca ggccgacgag
     4441 accctggaat ggctggagac gggacgcgtc gacgtgctgc gcttcgcgcg cccgaacggc
     4501 tggcagatcg tcaccacgtt cggtgccgag ccgttcgatc tcggcgagga cgccgcgcgc
     4561 gtggcgctga gcagccttcc cctcgtcgga ggggcgatgc ccggcgaggc gaccgcctgg
     4621 atcgctcccg gcgtctgacc ccggccgcac atggaggtgc gcc
//
LOCUS       DATMSX010000200         6429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_163729,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000200 DATMSX010000000
VERSION     DATMSX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163729"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VN041_06210"
     CDS             complement(<1..314)
                     /locus_tag="VN041_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28675.1"
                     /translation="MGNGRARRIAEEMLALLLAAACPGCDRTGELLCDGCRRMLIADP
                     LDLSTPGGLLVHAALRYEGVPARCIRRVKEEGATALARPWGVACGFRREVSHPSMCTR
                     ASG"
     gene            complement(408..2114)
                     /locus_tag="VN041_06215"
     CDS             complement(408..2114)
                     /locus_tag="VN041_06215"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HWU28676.1"
                     /translation="MRRIRGIRLLAVAVLGLLLAGCTGLPTSGPPNPGLAIGGEEDTL
                     IYTPIASGPKPGDSPEGIVKGFLEASMTPADNWEIAQEFLTDDFRPTWKPGTLVTIDE
                     SVLQRKFQSSTDSEDKSAKNAEVHAQFDQVANVDASGAYNAESGLGKATYQLKRVKGE
                     WRIAKAADGIVLDAETFGQVYQKYALKYFDTSWSHLVPDVRWFPRRTAMATSVARALI
                     SGKPSDWLAPAVRNAFTEDITLAGDAVTVDSSQVATVALSRSALSASASDLARMRTQL
                     EGSLSGAGVAEVRFTVDGAPLEAGTVPLDENIQEAGVLVQNAETFGRAVSGDTIEPVG
                     GLTAQIAKITAPIRAVDVSLDATLASVQLQDGTVWALADGNPTKLDVRADLIEPSLDP
                     FGFTWTVPRGAPRELQAWGVQGPTSHPIANAWPPYDSISQLRVSADGARVAAVATQAG
                     QRQLVVAAVLRGKSSEPTELGTPHVVSLLPGPTLGLGWVGSDTLAVLSSTPDPALTMY
                     PVGGPSITTAPPDAAAALAGAKATTGLRVLGSDGSVYAQRGSSWQVSVEGIRVLGTRA
                     GY"
     gene            complement(2111..3754)
                     /gene="mtrB"
                     /locus_tag="VN041_06220"
     CDS             complement(2111..3754)
                     /gene="mtrB"
                     /locus_tag="VN041_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HWU28677.1"
                     /translation="MDWRSPRSWPEAVIAEWRHSMRFRATVITLVATALTILVTCVIM
                     GLVIRNDLFEARKDEALHNSRAAVAQAQRVLESGVAGDDRATLSGMWRQAQNELGRAA
                     SSKLIVGVVSEAFGEGYIPPGFNSQQFSPAQLSGELITRVRDVPARQAWQSIALSHSD
                     APDSAGIVVGQILEIRGVARYEVYIAYDLADTDETLQFVQRTLWLAGLALVTLIAGIL
                     WLVLRSVSVPIVEAAETSARLASGDLGVRLRVHGADELASLGHSFNAMADSIESQIKE
                     LAELSLVQQRFVSDVSHELRTPLTTIRLAAEMINDQKEQFDPTTARTAELLHTQVQRF
                     ETLLSDLLEISRYDAGSVQLEVEATSLAQLAEELVEQMRPLAETHGIELRMVAPGGYS
                     PVDMDPRRVRRVLRNLIGNAIEHGEGQPVVVTVDSNQHAVAVGVRDFGMGMRPEDAEH
                     VFDRFWRADPSRKRTIGGTGLGLSIALGDARLHGGTLQVWSELGVGSNFVLTIPRAGG
                     EATGQGPIPLEPPETLADLGSVTQPIVVRRPDPEGGDRS"
     gene            complement(3796..4476)
                     /gene="mtrA"
                     /locus_tag="VN041_06225"
     CDS             complement(3796..4476)
                     /gene="mtrA"
                     /locus_tag="VN041_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HWU28678.1"
                     /translation="MTSRILVVDDDTALAEMIGIVLRTEGFDVLFCADGAQAVDEWRT
                     SRPDLVLLDLMLPGMDGIEICTRIRAESGVPIIMLTARTDTADVVRGLEVGADDYMVK
                     PFNPKELVARIRTRLRPAPQASAEVLRVGDLTVDVDAHEVRRGSTPIALTPLEFQLLV
                     ALASKPQQVFSREMLLEQVWGYHYKADTRLVNVHVQRLRAKVELDPDNPRIVMTVRGV
                     GYRAGSVS"
     gene            4646..6133
                     /locus_tag="VN041_06230"
     CDS             4646..6133
                     /locus_tag="VN041_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28679.1"
                     /translation="MSGQTWTPAPKKGAIPLHPMTFGMVLGRAFAALRHNPKVLFGFA
                     IVVEFIVTLATVGVLILMVWFIAVRIQSVPPSSPDYWPILIGSTALGGLAAVVMGLAS
                     VAFTSVVQGLVAADVSFATVSRKASLRLLWSRVKPAFWRLFSYSLLQGLAALLWVALL
                     FGVVGGVLAALGPDNGGAIALAVILGLLLGLGSIPLYVWLTTKLLVVPAVLVLERATI
                     RSAVVRSWRLIRGRFWFAFGVMFVIGAIMGIAVQVVAMPASLFSGIIGGILSPTGGGD
                     ETTAMIAVVLANVIPQVLVLAVQAIALVVTGTGGTLIYIDSRMRYEGLDQALIRYVEL
                     SAQGTPDDQLGDPFAVDPERAVSKNPPPVQKPAYAAYPGQYPSPGQYPYPAQGYPAPP
                     AYGAQPSYGQPAYGAQPAYPQAPYGAQPGYPATAAHPGGAQVPYTAPGGAQVPSSAPA
                     SPYAAPPAPPASEQQPPAYDPPAPQPPHVIPPSSASPWAPPGGDA"
     gene            6130..>6429
                     /locus_tag="VN041_06235"
     CDS             6130..>6429
                     /locus_tag="VN041_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28680.1"
                     /translation="MILLPLADVFVPDGDEARRQAAEELSKQEYQAAKPTWFDEFVAG
                     IVQWFVNLFTQNGAGGVAPFALTLIIVVVIAALVVALLVWGRPRASRTVARRRDLL"
BASE COUNT          903 a         2319 c         2204 g         1003 t
ORIGIN      
        1 cctgacgcgc gcgtgcacat gctgggatgg ctcacttcac ggcggaaccc gcatgcaaca
       61 ccccaaggac gggccagcgc ggtcgccccc tcttccttga cacggcggat gcagcgcgcc
      121 ggcacgccct cgtacctcag cgccgcgtgc acgaggagcc cgccgggcgt gctcaggtcg
      181 agcggatcgg cgatcagcat ccgccggcac ccgtcgcaca gcagctcccc cgtccgatcg
      241 caacccgggc acgcggccgc cagcagcagc gccagcatct cctcggcgat ccgtcgcgcg
      301 cgtccgttcc ccatccgacg atcatggcgc cgcccgcgtc gcacacgcgc gccggttcac
      361 ggcatccgtg cacaactcct ccagggcggg tgatgtgcag gagcagttca gtacccggcg
      421 cgggtcccca gcacgcggat gccctcgacg gacacctgcc acgacgaccc gcgctgtgcg
      481 tagacggagc cgtccgaccc gagcacgcgc agccctgtcg tcgccttggc tccggcgagc
      541 gctgctgcgg cgtccggcgg cgcggtcgtg atggaaggac cgcccaccgg gtacatcgtc
      601 aatgcggggt ccggcgtgct gctgagcacc gccagggtgt ccgagccgac ccaccccagg
      661 ccgagcgtcg gccccggaag caggctcacc acgtgcggtg tgcccagctc ggtgggctcg
      721 gacgacttgc cgcgcagcac cgcggccacg accagttggc gctgccccgc ctgggtcgcg
      781 accgcagcca cccgcgcacc gtcggcggag acccggagct ggctgatgga gtcatagggc
      841 ggccaggcgt tggcgatcgg atgcgacgtc gggccctgaa ctccccacgc ctgcagctcc
      901 cgcggcgcac cacgcgggac agtccaggtg aagccgaacg gatccaggga cggttcgatc
      961 aggtcggcgc gcacgtcgag cttggtgggg ttgccgtccg ccagggccca cacggtgccg
     1021 tcctgcagct gcacggaggc gagcgtcgca tccagcgaca cgtccacggc gcggatcggc
     1081 gcggtgatct tggcgatctg agctgtcagt ccgccgacgg gttcgatcgt gtcacccgac
     1141 acggcgcgac cgaacgtctc ggcgttctgc acgagcactc cggcctcctg gatgttctcg
     1201 tccaggggga ctgtgccggc ctccagcggt gcgccgtcga cggtgaagcg cacttcggcg
     1261 actccggcac ccgacaggct cccctccagc tgcgtgcgca tacgcgcgag atcggatgcc
     1321 gaagccgaca gcgcgctgcg cgagagcgcc accgtcgcca cctgcgagga gtcgacggtc
     1381 acggcgtcgc ccgcgagagt gatgtcctcg gtgaaggcgt tccgcaccgc gggcgccagc
     1441 cagtcgctgg gcttgccgga gatcagcgcc cgcgcgaccg acgtcgccat cgccgtgcgc
     1501 cgcgggaacc agcgcacatc ggggaccagg tgcgaccagc tggtgtcgaa gtacttcagc
     1561 gcgtacttct ggtagacctg tccgaacgtc tcggcatcca gcacgattcc gtcggcggcc
     1621 ttggcgatcc tccactcgcc cttgacccgt ttgagctgat aggtggcctt gcccagcccg
     1681 gactccgcgt tgtacgcgcc cgaggcatcg acgttcgcca cctgatcgaa ctgggcgtgc
     1741 acctccgcgt tcttggcgga cttgtcctcg gagtcggtcg aggactggaa cttccgctgc
     1801 agcacggact catcgatcgt cacgagagtg cccggtttcc aggtcggccg gaagtcgtcg
     1861 gtgaggaact cctgcgcgat ctcccagttg tccgccgggg tcatcgaggc ctccaggaag
     1921 cccttcacga tcccctccgg actgtcaccc ggcttggggc cggacgcgat cggcgtgtag
     1981 atcagcgtgt cctcctcgcc cccgatcgcg aggccggggt tcggcggccc cgatgtgggc
     2041 agccccgtgc agccggcgag cagcaggccg agaacggcga cggcgagaag acggatgccg
     2101 cggatgcgcc tcatgagcga tcgccacctt ccgggtcggg acggcgcacg acgatcggct
     2161 gcgtgacgct gccgaggtcg gcgagggtct ccggcggttc gagcgggatg ggcccctgcc
     2221 cggtggcctc gccgccggca cgagggatcg tgagcacgaa gttgctgccc acgccgagtt
     2281 cggaccacac ctgcagagtg ccgccgtgca gtctggcgtc gcccagcgcg atcgagagcc
     2341 cgagccccgt cccgccgatg gtgcgcttgc gcgaggggtc ggcgcgccag aagcggtcga
     2401 acacgtgctc ggcgtcctcg ggacgcatgc ccatcccgaa gtcgcgcacg cccacggcga
     2461 cggcgtgctg attgctgtcg accgtgacca ccaccggctg cccctccccg tgctcgatcg
     2521 cgttgccgat gaggttgcgg agcacccgac gcacccggcg cgggtccatg tcgacaggcg
     2581 agtacccgcc gggggccacc atccgcagct ctatgccgtg cgtctccgcc agcggcctca
     2641 tctgctcgac cagctcctcg gccagctgcg cgaggctggt cgcctcgacc tcgagctgca
     2701 cggatccggc gtcgtagcgg ctgatctcca gcaggtccga gagcagcgtc tcgaagcgct
     2761 gcacctgggt gtgcagcagc tcggccgtcc gcgcggtggt cgggtcgaac tgctccttct
     2821 gatcgttgat catctcagcg gccagccgga tggtcgtgag cggcgtacgc agctcgtgcg
     2881 agacgtcgga cacgaaccgc tgctgcacga gcgagagctc ggcgagctcc ttgatctgcg
     2941 actcgatgct gtcggccatg gcgttgaagg agtgcccgag gctggccagc tcgtcggcgc
     3001 cgtgcacgcg cagccgcacg ccgaggtcgc ccgacgcgag cctcgcgctg gtctccgcgg
     3061 cctcgacgat cggcaccgac acggatctga gcaccagcca gaggatgccc gcgatcaggg
     3121 tgaccagcgc gagtcctgcg agccacaggg tccgctgcac gaactgcagg gtctcgtcgg
     3181 tgtcggcgag gtcgtacgcg atgtagacct cgtagcgcgc gacccccctg atctcgagga
     3241 tctggcccac gacgatcccc gccgaatccg gagcatcgct gtgcgagagc gcgatggact
     3301 gccacgcctg ccgggccggg acgtccctca cacgggtgat cagctcgccg ctgagctgcg
     3361 cggggctgaa ctgctggctg ttgaagccag gcgggatgta gccctcgccg aatgcctcgg
     3421 acaccacgcc cacgatgagc ttcgacgacg ccgcgcggcc gagctcgttc tgcgcctgac
     3481 gccacatgcc ggacagggtg gcacgatcgt cgcccgccac accggactcg agcactcgct
     3541 gcgcctgcgc cacggcggcg cgcgagttgt ggagcgcctc gtccttgcgg gcctcgaaca
     3601 ggtcgttgcg gatgaccagg cccatgatga cgcaggtgac gaggatggtc agcgccgtgg
     3661 ccaccagggt gatgacggtg gcgcggaacc gcatggagtg tcgccattcg gcgatcactg
     3721 cctcgggcca gctgcgcggg gaccgccagt ccacggcctg cacccgcgtc gcggtcgtcg
     3781 cgatcacgag aactcctagc tgacgctccc ggcgcggtac ccgacgccgc gcacggtcat
     3841 gacgatgcgc gggttgtccg ggtcgagctc gaccttggca cgcagccgct gcacgtgcac
     3901 gttcaccagg cgcgtgtcgg ccttgtagtg gtagccccag acctgctcga gcagcatctc
     3961 gcgggagaag acctgctgcg gcttggaggc cagcgcgacc agcagctgga actccagcgg
     4021 ggtcagcgcg atcggcgtgg atccgcggcg cacctcgtgg gcgtcgacgt cgacggtgag
     4081 gtcgcccacg cgcagcacct cggcgctcgc ctgcggagcg ggacggaggc gggtccggat
     4141 gcgggcgacg agctccttcg ggttgaaggg cttgaccatg tagtcgtccg cgcccacctc
     4201 gagccctcgg acgacatcgg cggtgtcggt gcgcgcggtg agcatgatga tcggcacgcc
     4261 ggactcggcg cggatgcggg tgcagatctc gatgccgtcc atgccgggga gcatgaggtc
     4321 cagcagcacg aggtcgggac gcgaggtgcg ccactcgtcc acggcctgcg ccccgtccgc
     4381 gcagaacagc acgtcgaatc cctcggtgcg cagcacgatc ccgatcatct cggcgagcgc
     4441 cgtgtcatcg tccaccacga ggatccgtga ggtcatcttc tgcccagcct catctttctc
     4501 tgcgaagcag cgcgtcgatt cccctgccgc gcgcgactcg gatcagccta cttgagaaac
     4561 cgggtcccgt accggagtgg atcgcgtgcc cacgcaatcc acgcgccctg tgccacgatg
     4621 gacatgcaca tggaggggag tgccgatgag cggtcagacc tggacgcccg cgcccaagaa
     4681 gggcgccatc ccgttgcatc cgatgacctt cggcatggtg ctcggccggg ccttcgccgc
     4741 cctgcggcac aacccgaagg tgctgttcgg gttcgcgatc gtggtcgagt tcatcgtgac
     4801 gctggccacc gtgggcgtgc tgatcctcat ggtctggttc atcgcggtgc gcatccagtc
     4861 cgtgccgccc tcctcccccg actactggcc gatcctcatc gggtccacgg cgctgggtgg
     4921 cctggccgcg gtcgtcatgg gcctggcatc cgtcgccttc acctcggtgg tgcaagggct
     4981 ggtggccgcc gacgtctcgt tcgcgacggt gtcccgcaag gcctcgctgc gtctgctctg
     5041 gtcgcgggtg aagcccgcgt tctggaggct gttctcctac tcgctgctgc agggccttgc
     5101 cgcgctcctc tgggtggccc tgctcttcgg cgtggtgggc ggcgtcctcg cggccctcgg
     5161 ccccgacaac ggcggcgcga tcgcactggc cgtgatcctg ggactgctgc tggggctcgg
     5221 cagcatcccg ctgtacgtgt ggctgaccac caagctcctg gtggtccccg ccgtgctcgt
     5281 gctcgagagg gcgacgatcc gcagcgccgt ggtgcgctcc tggcggctca tccgcggcag
     5341 gttctggttc gccttcggcg tgatgttcgt gatcggcgcg atcatgggca tcgcggtgca
     5401 ggtcgtggcg atgccggctt ccctgttctc cggcatcatc ggcggcatcc tgagcccgac
     5461 cggtggcggc gacgagacga ccgcgatgat cgcggtcgtg ctcgcgaacg tcattccgca
     5521 ggttctcgtg ctcgcagtcc aggcgatcgc actggtcgtc acgggcaccg gcggcacgct
     5581 gatctacatc gacagccgga tgcggtacga gggactggac caggccctga tccggtacgt
     5641 cgagctcagc gcgcagggca ctccggacga ccagctcggc gacccgttcg ccgtggaccc
     5701 cgagcgcgcg gtgtcgaaga accccccgcc cgtgcagaag ccggcctacg ccgcctatcc
     5761 gggccagtac ccgtcccccg gtcagtaccc gtaccccgcg cagggctatc cggcaccgcc
     5821 cgcgtacggg gcccagccgt cgtacggtca gccggcatac ggcgctcagc cggcgtatcc
     5881 gcaggccccg tacggcgccc agccgggata cccggcgacc gccgcgcatc ccggtggggc
     5941 gcaggttccg tacacggcac ccggtggggc gcaggttccg tcctccgcac ccgcatcccc
     6001 gtacgcggct ccgccggctc ccccggcatc cgagcagcag cccccggcgt acgacccgcc
     6061 cgccccgcag cctccgcatg tgatcccgcc ctcctccgcc agcccgtggg ctcccccggg
     6121 cggagacgca tgatcctgct ccccctcgcc gacgtcttcg tccccgacgg ggacgaagcg
     6181 cggcgtcagg ccgcagagga gctgtcgaag caggagtacc aggccgccaa gccgacctgg
     6241 ttcgacgagt tcgtcgccgg catcgtccag tggttcgtga atctgttcac gcagaacggc
     6301 gcaggtggtg tggcgccgtt cgccctgacg ctgatcatcg tcgtcgtgat cgcggcactg
     6361 gtcgtggcgc tgctggtgtg ggggcgcccc cgcgcctcgc gtaccgtggc gcgccgccgc
     6421 gacctgctc
//
LOCUS       DATMSX010000201        10466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_116668,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000201 DATMSX010000000
VERSION     DATMSX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116668"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VN041_06240"
     CDS             complement(<1..651)
                     /locus_tag="VN041_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU28681.1"
                     /translation="MSVIDVKLIAQCLASLRDLERATISDLAESTGLSRPTVTAALTA
                     MTDSGLAIEHEARAAAGRPARTFSPVAGSPVIVGIDLTRSTVRLIVADRTGRVLGRQE
                     AEVASPTTDQPSLAPVRALLQQTLERIGRRQSDVAAIGIAAAGLIDENGILISAPQVT
                     SWNGRDLAAMFSDHFGAPVVVDNDLSLAAEAESRGGALTDAETGVYALTWHHVSARV"
     gene            782..2833
                     /locus_tag="VN041_06245"
     CDS             782..2833
                     /locus_tag="VN041_06245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWU28682.1"
                     /translation="MIEATNPLLSPSALPYGLPDYAAIRPEHYLRAFRQAFEEQLREV
                     RAITVVRSMPTFENTLVALERSGELLDRVAHAFYTVSSADATPEIQAIDEELAPLMAA
                     HEDAIRLDSQLYWRVSQVHAQLDELELDAEQRYLVQRHFREMTHAGAALDDEAKKRLT
                     ELNKRLSTLSTAFEKNLLNDTNDLAVHFDDASELDGLTSGELSATARAASDRGEDGRF
                     LVGLPLFSGHPYLSSLTVRESRRRILEASLSRGSRGNANDNGAVLTEIVRLRAERAAL
                     LGYPSHAAYITADETAGSPDAVERMLRDLAAPAARNASREAEALQEIIDATEPEPFAL
                     EAHDWAFYTEKVRQARYDIDTAALRPWFEAERVLRDGVFFAAGQLYGVSFTEREDLVA
                     YHPGARVFEVHDADGSAVGLYVLDLYTRDSKRGGAWMNPIVSQSALRGTLPVVVNNLN
                     VARPGDGDPTLLTLDEVTTLFHEFGHALHGLFARVTYPHFAGTNVFRDFVEFPSQVNE
                     MWILWSEVLDNYARHFETGEPLDAAIVERLRATEAFDQGHATSEYLAAAWLDQAWHSI
                     AAGAEVDDVAAFEAAALSDIGLLNPLVPTRYSSTYFAHVFSGGYSAGYYSYIWSEVLD
                     ADTVEWFRSNGGLTRANGDRFRQRLLGVGGSKDPLEAYRDFRGRDAEIAPLLKRRGLD
                     G"
     gene            complement(2934..4202)
                     /gene="clpX"
                     /locus_tag="VN041_06250"
     CDS             complement(2934..4202)
                     /gene="clpX"
                     /locus_tag="VN041_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWU28683.1"
                     /translation="MARIGESADLFKCSFCGKSQKQVQQLIAGPGVYICDECVELCNE
                     IIEERMAEAGSGPVADFELPKPREIFAFLEEYVVGQELAKRSLAVAVYNHYKRVRARG
                     TLQTAEQKAEEVEVAKSNILMLGPTGCGKTYLAQTLAKRLNVPFAVADATALTEAGYV
                     GEDVENILLKLLQAADYDVKRAETGIIYIDEIDKIARKAENPSITRDVSGEGVQQALL
                     KILEGTVASVPPQGGRKHPHQEFLQIDTTNVLFIVAGAFAGLEDIISARVGKHGVGFG
                     APLHDKGKDLDLFGEVLPEDLHKFGLIPEFIGRLPVVTSVSPLDQDALIDILTGPRNA
                     LVKQYQRMFEIDGVQLEFEEDALRSIADLAVLRKTGARGLRAILEDVLGPIMFEIPSA
                     EDVTKVIVTKAAVEEGAAPTVVLSRKRKSA"
     gene            complement(4355..5221)
                     /locus_tag="VN041_06255"
     CDS             complement(4355..5221)
                     /locus_tag="VN041_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU28684.1"
                     /translation="MSKRLTVPKTYKLAIGGAFPRSESGRTYEVVSAKGAFLANAAKA
                     SRKDARDAVVAARGAVKGWSGATAYNRGQVLYRVAEILEGRRAQFVDEIVDQEGVSAA
                     AAGAQVDAAVDLWVWYAGWCDKYAQVGGNANPVAGPYFNISVPEPTGVVAIVAPQDSA
                     LLGLVSVIAPALVTGNTVVAIASELFPLSAISLAEVLATSDVPGGVVNVLTGSPAEIT
                     PWLASHADVNAIDLAGAGDLDWVDLEIAAADTLKRVLRPGVGSNGAVEQSLDRITAFT
                     ETKTVWHTKSMV"
     gene            complement(5218..6711)
                     /locus_tag="VN041_06260"
     CDS             complement(5218..6711)
                     /locus_tag="VN041_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU28685.1"
                     /translation="MSFLEYAPAPESKAILKLKDSYGLFIDGEFVDGSGPSFHTISPA
                     DESLIAEIASASDEDVDRAVVAARRAYDRVWSKMSGRDRGKYLFRIARLVQERARELA
                     VAESLDNGKPIKESRDVDVPLVAAWFFYYAGWADKLDYAGLGADPKALGVAGQIIPWN
                     FPLLMLAWKLAPALAAGNTVVLKPAETTPLTALIFAEILQQADLPAGVVNIITGAGAP
                     GATLVRHPDVDKVAFTGSTGVGRDIARAIAGTDKRVTLELGGKAANIVFDDAPIDQAV
                     EGIVNGIFFNQGHVCCAGSRLLVQESIHDEVVDRLKHRLSTLRLGDPLDKNTDIGAIN
                     SAAQLARIRELSDIGEAEGAERWTADCAIPEKGFWFAPTIFTGVEASSRIARDEIFGP
                     VLSVLTFRTPAEAIAKANNTPYGLSAGIWSDKGSRILAVADRLRAGVVWANTFNRFDP
                     SSPFGGYKESGYGREGGRHGLTAYLKSAGPAETRAITAGATKKGASA"
     gene            complement(6722..7723)
                     /gene="deoC"
                     /locus_tag="VN041_06265"
     CDS             complement(6722..7723)
                     /gene="deoC"
                     /locus_tag="VN041_06265"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWU28686.1"
                     /translation="MTTTEIARRTAVDVLGGEPDDATLRRFLHGLPGVDAVGLEQRAA
                     GLGTRSIKTTSKAWALDKIISLIDLTTLEGADTAGKVRSLVAKAKTPDASDPTTPRVA
                     AVCVYGDMVGHAVEALGSLHGDPDEGRISVAAVATAFPSGRASIEVKLADTADAVAAG
                     ADEIDMVIDRGAFLEGRYGLVFDQIARVKEACRREDGSYASLKVIMETGELNTYDNIK
                     RASWLSILAGGDFIKTSTGKVQPAATLPTTLLMLETVRDWHRATGEKIGVKPAGGIRS
                     SKDAIKYLVTVAETAGEQWLQPHLFRFGASSLLNDVLLQRQKTTTGHYSGADYVTID"
     gene            complement(7720..8655)
                     /locus_tag="VN041_06270"
     CDS             complement(7720..8655)
                     /locus_tag="VN041_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HWU28687.1"
                     /translation="MDDELLMVRVAELSYDENKTQDEIGALLGISRWKVGRLLVQARE
                     RGIVRIEIVHPRARRLGAERALVERYGLKDAVVVPAPDDQGAVLERVARAAADMLTAL
                     RPVPRTLAVSWGRTLTAIADALPEGWTRGVTVVQANGGVSLNRRSGGAASVAVTIAQR
                     GAGQAVLLPSPAILERVETKQAIEADRTVAAILDQAAHADTFLFTAGPCDPASAHVEN
                     GYLSAEDIAELARRGAVGDLLGRYIDADGNIVDPQLDARTVGVGLDQVRNAPRSIFVT
                     AGAAKHDIARTVVSNGLCSVLVTDESTALALLEEI"
     gene            complement(8811..9452)
                     /locus_tag="VN041_06275"
     CDS             complement(8811..9452)
                     /locus_tag="VN041_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWU28688.1"
                     /translation="MYTPTFRAAGNLPSSRYVLPQFEERTAYGFKRQDPYNKLFEDRV
                     IFLGVQVDDASADDVMAQLLVLESQDSERDITMYINSPGGSFTAMTAIYDTMQYVAPQ
                     IQTVVLGQAASAASVLLAAGAPGKRLALPNARVLMHQPAMGEAGQGQASDIEIQAAEI
                     LRMRTWLEETMAKHTGQDVAKVNRDIDRDNILSAEQAKEYGIVDQVLTSRKRA"
     gene            complement(9468..10106)
                     /locus_tag="VN041_06280"
     CDS             complement(9468..10106)
                     /locus_tag="VN041_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWU28689.1"
                     /translation="MSPVDSNHRNTETGADMAEPLMATSVFDRLLKDRIIWLGSEVRD
                     ENANEICAKILLLAAEDPEKDIYLYINSPGGSITAGMAIYDTMQFVPNDIVTVGIGMA
                     ASMGQLLLTSGTKGKRYITPNARVLLHQPHGGFGGTASDIQTQAQLILSMKQRLAEIT
                     AGQTGKTVEQINEDGDRDRWFTADEALEYGFVDHIREHASDVHGGGGTGADK"
     gene            complement(10147..>10466)
                     /locus_tag="VN041_06285"
     CDS             complement(10147..>10466)
                     /locus_tag="VN041_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU28690.1"
                     /translation="PLYRAALEAGLDPVRRTRTVIQLASTIRNLGKLDEALAMLSAEY
                     DATRGGAMADEAAAFYALALTSSGDPVRAASVALTALAPHLSRYTRSVTGYAAELLDP
                     RGE"
BASE COUNT         1529 a         3531 c         3682 g         1724 t
ORIGIN      
        1 gacgcgagcg gacacgtggt gccaggtcag ggcgtacacg ccggtctccg cgtcggtcag
       61 ggcgccgccg cgggactcgg cctcggccgc gagggacagg tcgttgtcga cgacgacggg
      121 ggcaccgaag tggtcgctga acatcgccgc gaggtcgcgt ccgttccagc tcgtgacctg
      181 gggggcggag atcaggatgc cgttctcgtc gatgagcccc gcagcggcga tgccgatggc
      241 cgcgacgtcc gactgccgcc gcccgatccg ctccagggtc tgctgcagca gcgcacggac
      301 gggggcgagc gagggctggt ccgtggtggg cgaggcgact tccgcctcct ggcgtcccag
      361 cacgcggccg gtgcggtccg cgacgatcag gcgcacggtg ctgcgcgtca ggtcgatccc
      421 gacgatgacg ggtgaccctg cgaccggcga gaacgtccgg gcgggccgcc cggcggcggc
      481 acgcgcctcg tgctcgatcg cgagtccgga gtcggtcatg gcggtgaggg ccgcggtgac
      541 ggtcggccgg gagaggccgg tgctctcggc gaggtcggag atggtggcgc gttccagatc
      601 gcgcaggctg gcaaggcact gggcgatgag cttcacgtcg atcacagaca tcggcggacc
      661 cttctccttt gaaagacact ttctaagctc ctttcataac cgcttacgaa agtgctttca
      721 tcattgtggg gccccgaccg ccgactgtca aaccccgcgg accaacccgt aggctcgagg
      781 gatgatcgag gccacgaacc cgctgctgtc cccctccgcg cttccctacg gactgcccga
      841 ctacgcggcc atccgcccgg agcactacct gcgggcgttc cgtcaggcgt tcgaggagca
      901 gttgcgcgag gtccgggcga tcacggtggt gcgctcgatg ccgaccttcg agaacacgct
      961 ggtcgcactg gagcgttccg gcgagctgct cgaccgcgtc gcgcacgcct tctacaccgt
     1021 gagctccgcc gacgcgacgc ccgagatcca ggcgatcgac gaggagctgg ctccgctgat
     1081 ggccgcgcac gaggacgcca tccgcctgga ctcgcagctc tactggcgcg tttcgcaggt
     1141 gcacgcgcag ctcgacgagc tggagctgga tgccgagcag cgctacctcg tgcagcgcca
     1201 cttccgcgag atgacgcacg ccggcgccgc cctcgacgac gaggccaaga agcggctcac
     1261 agagctcaac aagcgcctgt ccactctcag cacggctttc gagaagaacc tgctgaacga
     1321 caccaacgat ctcgcggtgc acttcgacga cgcgtcggag ctcgacgggc tgacctccgg
     1381 cgaactgagc gcgaccgccc gggcggcatc cgaccgcggc gaggacggga ggttcctggt
     1441 gggcctgccg ctgttcagcg gccacccgta cctgtcatcc ctgaccgtgc gcgagagccg
     1501 acggcgcatc ctcgaggcat ccctctcccg cggatcgcgc ggcaacgcga acgacaacgg
     1561 ggctgtgctc accgagatcg tgcgattgcg cgccgaacgc gccgcgctgc tcgggtaccc
     1621 ctcgcacgcc gcctacatca ccgccgacga gaccgccggc tcgccggacg ccgtggagcg
     1681 gatgctgcgc gatctggccg cgcccgcagc gcgcaacgcc tctcgcgagg ccgaggcact
     1741 gcaggagatc atcgacgcca ccgagcccga gccattcgcc ctggaggccc acgactgggc
     1801 gttctacacc gagaaggtgc gccaggctcg ctatgacatc gacaccgcgg ccctgcgtcc
     1861 gtggttcgag gcggagcgcg tgctgcgtga tggcgtgttc ttcgctgccg ggcagctgta
     1921 cggagtcagc ttcaccgagc gcgaggacct ggtcgcctac caccccggtg cccgggtgtt
     1981 cgaggtgcac gacgccgacg ggtcggccgt gggcctgtac gtgctcgacc tctacacgcg
     2041 cgactccaag cgcggcggcg cctggatgaa cccgatcgtg agtcagtccg ccctgcgcgg
     2101 cacgctgcct gtggtggtca acaacctcaa cgtcgcccgg cccggcgacg gcgatccgac
     2161 gctgctgacc ctcgacgagg tcacgacgct gttccacgag ttcgggcacg ccctgcatgg
     2221 gctgttcgcc cgggtgacct acccgcactt cgcgggcacc aatgtcttcc gcgacttcgt
     2281 ggagttcccc agccaggtca acgagatgtg gatcctgtgg agcgaggtgc tggacaacta
     2341 cgcgaggcac ttcgagaccg gcgagccgct ggatgccgcg atcgtcgagc gtctgcgcgc
     2401 caccgaggcc ttcgatcagg gtcatgcgac cagcgagtac ctggcggcgg cttggctgga
     2461 ccaggcgtgg cacagcatcg ccgccggagc cgaggtcgat gacgtcgccg ctttcgaggc
     2521 ggccgccctg tccgacatcg ggctgctgaa tccgctggtg ccgacccggt attcctcgac
     2581 ctacttcgcg cacgtgttct cgggcggcta cagcgccggc tactactcgt acatctggag
     2641 cgaggtgctc gacgccgaca cggtcgagtg gttccgctcg aacggcgggc tcacccgggc
     2701 caacggcgac cggttccggc agcgcctgct cggcgtgggc ggctcgaagg atccgctgga
     2761 ggcctaccgc gacttccgcg gccgggatgc cgagatcgcc ccgctgctga agcgtcgcgg
     2821 cctggatggc tgacggcaca gccggggtgt aactcggcag aaacgcacga ctccgcagga
     2881 atccggtcag gatctgcgga gttgtgcgtt tctgcgaggt tgcgcagcga aggtcaggcg
     2941 ctcttgcgct ttcgggagag caccacggtc ggggcagcgc cctcctcgac ggcggccttg
     3001 gtgacgatca ccttggtgac gtcctcggca gaggggatct cgaacatgat cggccccagc
     3061 acatcctcga ggatggcccg cagaccacgg gcgccggtct tgcgcagcac ggccagatcg
     3121 gcgatcgagc gcagcgcgtc ctcttcgaac tcgagctgca cgccgtcgat ctcgaacatc
     3181 cgctgatact gcttgaccag agcgttgcgc gggccggtga ggatgtcgat cagcgcgtcc
     3241 tggtcgagcg gcgacacgga ggtgacgacc ggcaggcggc cgatgaactc cgggatcagc
     3301 ccgaacttgt gcaggtcttc ggggagcacc tcgccgaaga gatcgaggtc cttgcccttg
     3361 tcgtgcagcg gggcgccgaa gccgacgccg tgcttgccga cgcgcgcgga gatgatgtcc
     3421 tcgagtcccg cgaaggcgcc cgccacgatg aacagcacgt tcgtcgtgtc gatctgcagg
     3481 aactcctgat gcgggtgctt gcgtccgccc tgcggcggca cggatgcgac tgttccctcg
     3541 aggatcttca gcagcgcctg ctgcacgccc tcgcccgaga cgtcacgggt gatcgacggg
     3601 ttctcggcct tgcgcgcgat cttgtcgatc tcatcgatgt agatgatgcc ggtctcggcg
     3661 cgcttgacgt cgtagtcggc ggcctgcagc agcttgagca ggatgttctc gacgtcttca
     3721 ccgacgtagc cggcctcggt gagcgcggtg gcgtcggcga cggcgaacgg cacgttcagc
     3781 cgcttggcga gggtctgcgc gaggtaggtc ttgccgcagc cggtcgggcc gagcatgagg
     3841 atgttgctct tggcgacctc gacctcctcg gccttctgct cggcggtctg cagcgtgccg
     3901 cgggcgcgca cgcgcttgta gtggttgtag acggcgaccg cgagggagcg cttcgcgagc
     3961 tcctgaccga cgacgtactc ctcgaggaag gcgaagatct cacggggctt gggcagctcg
     4021 aagtcggcga ccggaccgct gccggcctcg gccatgcgct cctcgatgat ctcgttgcac
     4081 agctcgacgc actcgtcgca gatgtacacg ccgggaccgg cgatcagctg ctgcacctgc
     4141 ttctggctct ttccgcagaa tgagcatttg aacaggtcgg cgctctcacc gatgcgtgcc
     4201 atgcgcgtcc tcctcgaagc tgtggatcag gcgggggctc tgatcccgtc aatcgagcct
     4261 aaccgcccgc accgacaccg gggcgtattg ccacgcatgc gaagggcccg gtccgccgtg
     4321 agcggaaccg ggcccccgag cgtccgatcg gaggtcagac catgctcttg gtgtgccaca
     4381 ccgtcttggt ctcggtgaac gccgtgatgc ggtccaggct ctgctcgacg gcgccgttcg
     4441 atccgacgcc ggggcgcagc acgcgcttga gcgtgtcggc ggcggcgatc tccaggtcga
     4501 cccagtccag gtctcccgca cccgccagat cgatggcgtt gacgtcggcg tgcgaggcga
     4561 gccacggcgt gatctccgcc ggcgatccgg tcagcacgtt gacgactccc ccgggcacgt
     4621 cgctggtggc gagcacctcg gccaggctga tcgccgagag cgggaagagc tcgctcgcga
     4681 tcgcgaccac cgtgttgccg gtgaccagcg ccggcgcgat gaccgacacc agcccgagca
     4741 gggcggagtc ctgcggggcg acgatcgcga ccacgccggt gggctcgggc accgagatgt
     4801 tgaagtacgg gcctgcgacc gggttggcgt tgccgcccac ctgcgcgtac ttgtcgcacc
     4861 agccggcgta ccagacccac aggtcgacgg cggcatccac ctgggcgccc gccgcagccg
     4921 cggagacgcc ctcctggtcg acgatctcgt cgacgaactg ggcgcggcgc ccctccagga
     4981 tctcggcgac tcggtacagc acctgcccgc ggttgtacgc ggtggcaccc gaccacccct
     5041 tcaccgcgcc gcgcgcggcg accacggcat ccctggcatc cttgcgcgaa gccttggcgg
     5101 cgttggcgag gaaggcgccc ttcgcggaga ccacctcgta ggtgcggccc gactcgctgc
     5161 gcgggaacgc cccgccgatc gcgagcttgt aggtcttcgg tacggtcagt cgcttgctca
     5221 tgccgatgct cccttcttcg ttgcgccggc ggtgatcgcg cgggtctcgg cgggccctgc
     5281 ggacttcaag tacgcggtca ggccgtgacg gccgccctcg cggccgtagc cggattcctt
     5341 gtagccgccg aacgggctcg acgggtcgaa gcggttgaag gtgttcgccc acaccacgcc
     5401 ggcgcgcaac cggtcggcga cggcgaggat gcgcgatccc ttgtcgctcc agatgccggc
     5461 cgacagaccg taaggcgtgt tgttcgcctt ggcgatcgcc tcggcgggcg tgcggaaggt
     5521 gagcaccgac agcaccggcc cgaagatctc gtcgcgggcg atgcggctgg acgcctcgac
     5581 gccggtgaag atcgtcggcg cgaaccagaa acccttctcc ggaatggcgc agtccgccgt
     5641 ccagcgctcc gcgccctccg cctcgccgat gtcgctgagc tcgcggatgc gggccaactg
     5701 ggcggccgag ttgatggcgc cgatgtcggt gttcttgtcg agcggatcgc ccaggcgcag
     5761 cgtcgacagg cggtgcttca gccggtcgac gacctcgtca tggatcgact cctgcaccag
     5821 cagccggctc ccggcgcagc acacgtgacc ctggttgaag aagatgccgt tcacgatgcc
     5881 ctcgacggcc tggtcgatgg gcgcgtcgtc gaacacgatg ttggcggcct tgccgcccag
     5941 ctcgagcgtg acgcgcttgt cggtgccggc gatcgccctc gcgatgtcgc ggccgacgcc
     6001 ggtcgaaccg gtgaacgcga ccttgtccac gtcggggtgg cgaacgagcg tggcgccggg
     6061 agcacccgca cccgtgatga tgttcacgac gcccgcgggc aggtcggcct gctgcaggat
     6121 ctcggcgaag atcagcgcgg tcagcggcgt ggtctcggcg ggcttcagca ccaccgtgtt
     6181 gccggccgcg agcgccgggg cgagcttcca cgccagcatg agcaacggga agttccacgg
     6241 gatgatctga ccggcgacgc cgagcgcctt ggggtcggcg cccaggccgg cgtagtcgag
     6301 cttgtccgcc cagcccgcgt agtagaagaa ccacgcggcg acgaggggca cgtcgacgtc
     6361 acggctctcc ttgatcggct tgccgttgtc caggctctcc gcgacggcca gctcgcgagc
     6421 gcgctcctgg acgaggcgcg cgatgcggaa caggtacttg ccgcggtcgc ggccgctcat
     6481 cttcgaccac accctgtcgt acgcacggcg ggcggcgacg acggcgcggt cgacatcctc
     6541 atcggaggcc gaggcgatct cggcgatgag gctctcgtcg gcgggtgaga tggtgtggaa
     6601 ggacgggccg gagccgtcga cgaactcgcc gtcgatgaac agtccgtagc tgtccttgag
     6661 cttcaggatc gccttggact ccggggcggg agcgtattcc aggaatgaca tatgaggctc
     6721 ctcagtcgat ggtgacgtag tcggcaccgg agtagtgacc ggtggtggtc ttctgacgct
     6781 gcagcagcac gtcattgagc aggctcgacg cgccgaagcg gaacaggtgc ggctgcagcc
     6841 actgctcacc tgcagtctcg gcgactgtga cgaggtactt gatggcgtcc ttcgacgagc
     6901 ggatgccgcc ggcgggcttc acgccgatct tctcccccgt cgcccgatgc cagtcccgga
     6961 cggtctcgag catgagcagc gtggtcggca gggtcgccgc gggctgcacc ttgccggtgg
     7021 atgtcttgat gaagtcgcct ccggcgagga tcgacagcca cgacgcgcgc ttgatgttgt
     7081 cgtaggtgtt cagctcgccg gtctccatga tgaccttgag cgaggcgtag ctgccgtcct
     7141 cgcggcggca ggcctccttc acgcgggcga tctggtcgaa cacgagcccg tagcgcccct
     7201 ccaggaacgc gccgcgatcg atcaccatgt cgatctcgtc ggcgcccgcg gccacagcat
     7261 ccgccgtgtc ggccagcttg acctcgatgg aggcgcgccc ggaggggaac gcggtcgcga
     7321 cggccgcgac ggagatgcgc ccctcgtcgg ggtcgccgtg cagggatccg agcgcctcga
     7381 cggcgtgacc gaccatgtcg ccgtacacgc agacggccgc cacgcgcggg gtcgtcgggt
     7441 cggaggcatc cggggtcttc gccttcgcga ccagcgagcg caccttgccc gcggtgtcgg
     7501 cgccctcgag ggtggtgagg tcgatcagcg agatgatctt gtccagcgcc cacgctttcg
     7561 aggtggtctt gatcgagcgg gtgccgaggc cggcggcgcg ctgctccagg cccacggcat
     7621 ccacgccggg cagtccgtgc aggaaccggc gcagggtggc gtcgtcgggc tcaccgccca
     7681 gtacgtcgac cgccgtgcgg cgggcgatct ctgtggtcgt catatctcct ccaagagtgc
     7741 aagggcagtg gattcgtcgg tcaccagaac gctgcagagg ccgttgctga cgactgtacg
     7801 cgcgatgtcg tgcttggcgg cgcctgcggt gacgaagatg gatcggggtg cgttgcgcac
     7861 ctggtcgagg ccgacaccca cggtgcgggc gtcgagctgc gggtcgacga tgttgccgtc
     7921 cgcgtcgatg tagcggccga gcagatcgcc caccgccccg cggcgggcga gctcggcgat
     7981 gtcctccgcc gacaggtagc cgttctcgac gtgagcggat gccgggtcgc acggccccgc
     8041 ggtgaacagg aacgtgtcgg cgtgcgcggc ctggtcgagg atcgccgcga cggtgcggtc
     8101 ggcctcgatg gcctgtttgg tctcgacacg ctccaggatc gccgggctgg gcagcagcac
     8161 cgcctgtcca gcgccgcgct gggcgatggt gacggcgacg gatgccgcac ctccggagcg
     8221 ccggttcagg ctcacgccgc cgttcgcctg gacgaccgtg actccgcgcg tccatccctc
     8281 gggcagcgcg tctgcgatcg ccgtgagagt gcggccccag ctgacggcga gggtacgggg
     8341 cacgggacgg agagcggtga gcatgtcggc ggcggcgcgg gcgacgcgct cgaggacggc
     8401 accctgatca tccggcgcgg gtacgaccac cgcatccttc agcccgtacc gctcgacgag
     8461 ggcgcgctcg gcaccgagcc tgcgggcacg cggatgcacg atctcgatgc ggacgatccc
     8521 ccgctcgcgg gcctgcacga gcagccgccc gaccttccag cgcgagatcc ccaggagggc
     8581 gccgatctcg tcctgcgtct tgttctcgtc gtacgagagc tcggcgactc gcaccatgag
     8641 cagttcgtcg tccacggcat cctccttccg tgtcccagct taggaggtga ccgacgcacg
     8701 cgcatcgctc tgctcagatg agcatggatg ccccgcgcgg ccgcgcatcc gtggggatac
     8761 gcggaagggc ggtccggcat gatgccggac cgccctttgc gatgatctgc tcaggcgcgc
     8821 ttgcgcgagg tgagcacctg gtcgacgatg ccgtactcct tggcctgctc ggccgagagg
     8881 atgttgtcac ggtcgatgtc gcggttcacc ttggcgacgt cctggccggt gtgcttggcc
     8941 atggtctcct cgagccaggt gcgcatgcgg aggatctccg cggcctggat ctcgatgtcg
     9001 gaggcctggc cctgtcccgc ctcgcccatc gccggctggt gcatgagcac acgggcgttg
     9061 ggcagagcga ggcgcttgcc gggagcgccg gcggccagca gcacggaagc cgcggatgcc
     9121 gcctggccca gcacgacggt ctggatctgc ggcgccacgt actgcatggt gtcgtagatc
     9181 gccgtcatgg ccgtgaacga gccgccgggc gagttgatgt acatcgtgat gtcccgctcg
     9241 gagtcctggc tctccaggac cagcagctgg gccatcacgt cgtcggccga cgcgtcatcg
     9301 acctgcacgc cgaggaagat cacacggtcc tcgaacagct tgttgtacgg gtcctggcgc
     9361 ttgaagccgt aggcggtgcg ctcctcgaac tggggcagca cgtagcggct ggacgggagg
     9421 ttgcccgcgg cgcggaaggt gggggtgtac atgtgtgtcg tcctgcctta cttgtccgcg
     9481 ccggttccgc caccgccgtg cacgtcactg gcgtgctcgc ggatgtggtc gacgaatccg
     9541 tactcgagtg cctcgtcggc ggtgaaccag cggtcgcggt caccgtcctc gttgatctgc
     9601 tcgacggtct tgccggtctg acccgcggtg atctcggcga ggcgctgctt catcgagagg
     9661 atcagctgcg cctgcgtctg gatgtcgctg gccgtgcctc cgaagccgcc gtgcggctgg
     9721 tgcagcagca cgcgcgcgtt cggcgtgatg tagcgcttgc ccttggtgcc gctggtcagc
     9781 agcagctggc ccatcgacgc cgccatgccg atgccgacgg tgacgatgtc gttcggcacg
     9841 aactgcatgg tgtcgtagat ggccatgccc gcggtgatcg agccgccggg cgagttgatg
     9901 tagaggtaga tgtccttctc gggatcctcg gcggcgagaa ggaggatctt cgcgcagatc
     9961 tcgttcgcgt tctcatcgcg cacctcagag cccagccaga tgatgcgatc cttcagcagt
    10021 ctgtcgaaga cgctcgtcgc catcaggggt tcagccatgt cagctcctgt ttccgtgttt
    10081 cggtgattcg aatctaccgg cgacaacgca ggcgcccggc cgtgttcgcc ctgagcacag
    10141 agcgtgtcac tcgccgcgcg ggtccagcag ctccgcggcg tatccggtga ccgagcgcgt
    10201 gtaccggctc aggtgcggcg cgagcgcggt cagcgcgacg gatgccgcac gcacggggtc
    10261 acccgacgag gtcagcgcca gtgcgtagaa cgccgcggcc tcgtcggcca tggcgccacc
    10321 gcgggtcgcg tcgtactccg cactgagcat cgcgagcgcc tcgtcgagct tgccgaggtt
    10381 gcggatggtg ctggcgagct ggatcacggt ccgggtgcgg cgcaccgggt cgaggccggc
    10441 ctcgagcgcg gctcggtaga gcggct
//
LOCUS       DATMSX010000202         9314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_182993,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000202 DATMSX010000000
VERSION     DATMSX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182993"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VN041_06290"
     CDS             <1..498
                     /locus_tag="VN041_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWU28691.1"
                     /translation="SIMLGGDDPDDVALAADVLAPCGTSVHLGPLGAGEVAKACNQLV
                     VSATILALGEATVLADRSGLDLDRMWRLLAGGYAGSRLLETRRDKLVSGDDSPSGMAK
                     YMVKDLGFASDIAAATGTRAALLPALRAAFDEIVDAGLGERDISVTRRLTAQRGAPAG
                     ETRES"
     gene            554..2002
                     /gene="gndA"
                     /locus_tag="VN041_06295"
     CDS             554..2002
                     /gene="gndA"
                     /locus_tag="VN041_06295"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWU28692.1"
                     /translation="MPEASANIGVVGLAVMGSNLARNLASREGNTVAVLNRSRTKTDE
                     LVAAHPEAGFIPAFSYEEFAASLQKPRTAIIMVKAGGPTDAVIDELVRVFEPGDIIVD
                     GGNAYFPDTIRREKAVRGTGINYVGAGISGGEEGALNGPSIMPGGSDESWVTLGPILK
                     SIAAVAEGEPCVTHIGHDGAGHFVKMVHNGIEYADMQLIAEAYDLIRRGTGKSPAEIA
                     EIFAEWNKGELESYLIEITAEVLRQVDAATGQPLVDVILDQAGAKGTGAWTVQTALSL
                     GTPVSGIAEATFARSLSSHPEQRAVAGALPGPDEAFTVADADAFIEDVRLALYASKIV
                     AYSQGFDEIRAGAAEYDWTIDLGAVSKIWRAGCIIRAQFLNRIADAYAEAPDLPVLMT
                     APYFAEAITRAQAAWRRVVVAAAQAGIPAPAFSSSLSYYDGIRADRLPAALVQGQRDF
                     FGAHTYKRIDKPGTFHTLWSGDRTEIEAEDTH"
     gene            2119..2847
                     /locus_tag="VN041_06300"
     CDS             2119..2847
                     /locus_tag="VN041_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU28693.1"
                     /translation="MTHEHPDLRRPDGSPLRILAVDDEPMLTDLLAMALRMEGWEVRT
                     AGSGLEALQVARDFEPDALVLDVMMPDLDGVGVLRRLREAGNLVPVVFLTAKDSVGDR
                     IAGLTAGGDDYVTKPFSLEEVIARLRAVIRRSGQGRADDEQSILRVADLSLNEDSHEV
                     MRGDDEIELTATEFELLRFLMRNERRVLSKAQILDRVWSYDFGGKSSVVELYISYLRK
                     KIDAGRTPLLHTVRGVGYMIKSPQ"
     gene            2855..4405
                     /locus_tag="VN041_06305"
     CDS             2855..4405
                     /locus_tag="VN041_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU28694.1"
                     /translation="MFARPVSLQARLMAAVIGFVSLILVVVAIITSATLGKTLEDRLQ
                     EQLNGTAATTKSVVTSQANRMLPLGGTFTAASALADYKVQGIDLLLMIVPPGMSASGV
                     VVTPDSRREISGADILTITAALRDANSATVSLPDFGSYRLTVSRTDNGVVVVTGLSRA
                     DVQRTMTMLFTVIALATLGGLLLLALTTALTISMGLRPLRAVAATATRVAGQPLDRGE
                     VSITERVPDYEADPRTEVGRVGAALNTLLDHVDSSLAARQRNEERMRRFVADASHELR
                     TPLASIRGYSELSLKAIRQAEQADADQSARTLENTTSALERIQAQSLRMTRLVEDLLL
                     LARLDEGRELVSREVDLAQLAVEALTDAQPTAPDHAWELDVPEDPVMLMGDAGRLHQV
                     LANLLANARTHTPAGTTITLSLAIESGTAELRVHDDGPGIDPQVRDELFARFARGDAS
                     RARQTGGTGLGLAIAKAIVEGHGGTIDVTSAPGDTTFTVRLPLETAPQQTVPEEFPPP
                     PPPAPPAQ"
     gene            complement(4399..5949)
                     /locus_tag="VN041_06310"
     CDS             complement(4399..5949)
                     /locus_tag="VN041_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28695.1"
                     /translation="MIPSLTALRQRVLAVLGGISMYRLVFFALVALAVVALGFSFTGL
                     VVPSAAEILASFAVLAIVISAVDAAAQRILRLPWRVESSLVTALILLFVMQPSLTAGG
                     LLGNALAGALASLSKYLIAWRGRHVLNPAAFGAAVVTVLGLGSRSSWWVGTPVLAVAV
                     AVLGLLVLWRIEKVRIVMVFLLVVVGVSVVRQALLAQSAGTEVAIADAVAFAIVHTPY
                     LFLGAFMLSEPLTQPPRRWQQFSVAALVGVLAGWPIPIGDLFTLGQERALLVGNLLAF
                     AFALRGSVRLVLEKRRMVTPTAQELTFRTKGRLSFLPGQYLELDVPHHRPDARGTRRE
                     FSIVSAPADLPILRIAYKNGDQKHPSSYKRALAAAEPGAVLAVTGTWGDFLLPRGGAP
                     TLMVAAGIGVTPFVSQLRQMQSLGVQRDVVLVYVAAEAAELAFRDELQATGVRAVVFT
                     RDEPADLAENWTWAQGVRLDAAGLERVVPDIAARHAFISGPPRLIADLAPALQKARSL
                     TTDAFAGY"
     gene            complement(5966..7000)
                     /locus_tag="VN041_06315"
     CDS             complement(5966..7000)
                     /locus_tag="VN041_06315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWU28696.1"
                     /translation="MGHKKKTVALDRENGVAPGLIAKTKKRLVVAGGRSHPELTAAVA
                     AALGQEMAPVEHRTFASGEIYARFEVSIRGCDLFIVQSFGEPVNEWLMETLIMLDAAK
                     RASAKRITVVAPYYPYSRQDKKGRGREPISARLVADMLKVAGADRVMSVDLHAAQIQG
                     FFDGPVDHLFAKPVLLKHFEETLTAEDRETLTIVSPDMGRVRVADTWSDSLNAPLAII
                     HKRRDPKVANQVSVHEIVGTVNDRTCLLVDDMIDTGGTIVKAAQALKANGARKVIVAA
                     THAIFSDPASVRLQDEAIDQVVVTDTIPLSESRRWDELVILPIAPLLATAIRQVFEDG
                     SVTSMFNGAA"
     gene            complement(7010..8440)
                     /gene="glmU"
                     /locus_tag="VN041_06320"
     CDS             complement(7010..8440)
                     /gene="glmU"
                     /locus_tag="VN041_06320"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWU28697.1"
                     /translation="MSENALAIIVLAAGQGTRMKSRLPKVLHEIGGRPLVGHVLTTAH
                     ALGAQHIEVVVRHEREQVIATLAEQYPDARIVDQDEIPGTGRAVQVALDALPDDFDGD
                     VLVLSGDVPLLEADTLRHLLDGHRAASAAATLLSAHLDDPTGYGRVIRGADGAVERIV
                     EQKDATDAEAAVTEINAGVYVFRVAALRTFLTRIDQNNAQGELYLTDVIGLLRGAAEH
                     VAAELAPDAASTFGVNDRAQLAEAGRVLNQRIVRRWQREGVTIIDPATTWIDDDVVLA
                     PDVTVLPNTQLLRACVIATGATIGPDTTLVDTEVGEHAVVKRTDANLAVIGARATVGP
                     FSFLRPGTVLGEGGKIGAYVETKNAQIGEGSKVPHLSYVGDAVIGRHVNLGASTITAN
                     YDDVNKHRTEIGDEVHTGSHTVLVAPVRLGAGAKTGAGAVVRKDVPAGSLAMSVAPQR
                     NIEGWVEKNRAGTGAADAAARERSAE"
     gene            complement(8442..8513)
                     /locus_tag="VN041_06325"
     tRNA            complement(8442..8513)
                     /locus_tag="VN041_06325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8479..8481),aa:Gln,seq:ttg)
     gene            complement(8601..>9314)
                     /locus_tag="VN041_06330"
     CDS             complement(8601..>9314)
                     /locus_tag="VN041_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HWU28698.1"
                     /translation="VAVDRPVVDPRGGVFCYALTEDRWVIGGAINNGGVTLDWVRQEI
                     ADATTPIEELLDRAGAVPVGSGGLLMLPYLLSERAPHWSSIASGAYIGLTRAHTRDHM
                     VRAAVEGVALQLALVLQSMRAAGLEIDEIRATGGVIKHPLWQQTIADAFGAPIGLLAG
                     HEGSGFGAALLGMQALGLIESFDVAAQLQPVERVVRPRPAESAVYRSLLPVFDEIYDA
                     LLPTFHRLRRLAPSLPVSH"
BASE COUNT         1436 a         3327 c         3233 g         1318 t
ORIGIN      
        1 tcgatcatgc tgggcggcga cgacccggac gacgtggcgc tggccgccga cgtgctggcg
       61 ccgtgcggca catccgtgca cctcggtccg ctcggtgcag gcgaggtcgc gaaggcctgc
      121 aatcagctcg tggtctcagc caccatcctg gccctgggcg aggcgaccgt gctcgcggat
      181 cgctccggcc tggacctcga tcggatgtgg cgactgctcg ccggcggcta cgcgggctcg
      241 cgcctgctcg agacccgacg cgacaagctg gtcagcggcg acgactcccc cagcggcatg
      301 gccaagtaca tggtgaagga cctcggcttc gcatccgaca tcgccgctgc caccggaacc
      361 cgggcggcgc tgctgcccgc gctgcgcgcg gcgttcgacg agatcgtcga cgccggcctc
      421 ggcgagcggg acatctccgt cacccggcgt ctgaccgcgc agcgtggagc gcccgcgggc
      481 gaaacgcgag agtcctgacc ctcccccagc ggataccctg aaaccacctg cacccacgat
      541 cgaggagctc tcagtgcccg aagcatcagc caacatcgga gtcgtcggac tcgccgtcat
      601 ggggtcgaac ctggcccgca acctcgcgag ccgcgagggc aacacggtgg cggtgctgaa
      661 ccgttcccgc accaagaccg acgagctggt cgccgcgcac cccgaggcgg gtttcatccc
      721 ggcgttctcg tacgaggagt tcgcagcgtc gctgcagaag ccgcgcaccg cgatcatcat
      781 ggtcaaggcg ggcggcccga cggatgccgt gatcgacgag ctcgtgaggg tgttcgagcc
      841 gggcgacatc atcgtcgacg gcggcaacgc gtacttcccc gacaccatcc gccgcgagaa
      901 ggcggtgcgc ggcaccggta tcaactacgt gggcgcgggc atctccggcg gcgaggaggg
      961 cgcgctcaac ggtccgtcca tcatgcccgg cggctcggac gagtcctggg tcacgctcgg
     1021 ccccatcctg aagtccatcg ccgcagtcgc cgagggcgag ccgtgcgtga cgcacatcgg
     1081 ccacgacggc gccggccact tcgtcaagat ggtgcacaac ggcatcgagt acgccgacat
     1141 gcagctcatc gccgaggcct atgacctgat ccgccgcggc acgggcaaga gccccgccga
     1201 gatcgccgag atcttcgccg agtggaacaa gggcgagctg gagtcgtacc tcatcgagat
     1261 caccgccgag gtgctgcgcc aggtggacgc cgccaccggc cagccgctcg tcgacgtcat
     1321 cctcgaccag gcgggcgcca agggcaccgg cgcctggacc gtgcagaccg ccctctcact
     1381 gggcaccccg gtctcaggca tcgccgaggc gacgttcgcc cgctcgctgt cctcccaccc
     1441 cgagcagcgc gcggtcgccg gggccctccc gggaccggac gaggcgttca ccgtcgcaga
     1501 cgcggacgcc ttcatcgagg acgtgcgcct ggcgctgtac gcgtcgaaga tcgtcgccta
     1561 ctcgcagggc ttcgacgaga tccgcgcggg cgcagccgag tacgactgga ccatcgacct
     1621 gggcgccgtg tccaagatct ggcgtgcggg ctgcatcatc cgcgcccagt tcctgaaccg
     1681 catcgccgat gcctacgccg aggcccccga cctgcccgtg ctgatgaccg cgccgtactt
     1741 cgccgaggcc atcacgcgcg cgcaggccgc ctggcgccgc gtcgtcgtgg ccgccgcgca
     1801 ggccggcatc cccgcccccg cgttctcgtc gtcgctgtcg tactacgacg gcatccgcgc
     1861 cgaccgcctc ccggccgcgc tcgtgcaggg ccagcgcgac ttcttcggcg cgcacaccta
     1921 caagcgcatc gacaagcccg gcaccttcca cacgctgtgg tccggcgacc gcaccgagat
     1981 cgaggccgag gacacgcact gatccccaca ccccactggg ctggatgagg gccggatgcc
     2041 gaacaggcat ccggccctca cagcatccgc atagccaacc ctatggattc tcgatgagtg
     2101 ggcgcgcaca atgagtgcat gactcacgag caccccgacc tgcgccgtcc ggacggctcc
     2161 cccttgcgca tcctcgccgt cgatgacgag cccatgctca ccgacctgct ggcgatggcg
     2221 ctgcgcatgg agggctggga ggtgcgcacc gcgggttcgg gcctggaggc tctgcaggtc
     2281 gcccgcgact tcgagccgga tgccctggtg ctggacgtca tgatgcccga cctcgacggc
     2341 gtgggagtcc tgcgccggtt gcgcgaggcc ggcaacctcg tgccggtggt gttcctcacc
     2401 gccaaggact ccgtcggcga ccgcatcgcg ggtctgacgg ccggcggcga cgactacgtg
     2461 accaagccgt tcagcctcga ggaggtcatc gcgcgactgc gcgccgtgat ccgccgttcc
     2521 ggccaggggc gcgccgacga cgagcagtcg atcctgcgcg tggccgacct gtcactgaac
     2581 gaggacagcc acgaggtgat gcgcggcgac gacgagatcg agctgaccgc caccgagttc
     2641 gagctgctgc gcttcctgat gcgcaacgag cggcgcgtgc tctccaaggc gcagatcctg
     2701 gaccgcgtgt ggagctacga cttcggaggc aagtcctcgg tcgtggagct gtacatctcg
     2761 tacctgcgca agaagatcga cgccggccgc actcctctgc tgcacaccgt gcgcggcgtc
     2821 ggctacatga tcaagtcccc gcagtagcgt gcacatgttc gcccgcccgg tgagcctgca
     2881 ggcgcggctg atggctgcgg tgatcgggtt cgtctctctg atcctggtgg tggtggccat
     2941 catcaccagc gccacgctcg gcaagactct cgaagaccgt cttcaggagc agctgaacgg
     3001 caccgcagcc acgacgaagt cggtggtgac ctcgcaggcg aatcggatgc tgcccctggg
     3061 cggaaccttc acggccgcca gcgccctggc ggactacaag gtgcagggca tcgacctgct
     3121 gctgatgatc gtgcccccgg ggatgtccgc ctccggcgtc gtggtgacgc cggactcgcg
     3181 tcgtgagatc tccggcgcgg acatcctgac gatcacggcc gcgctgaggg acgcgaactc
     3241 cgccacggtg agccttccgg acttcgggtc ctaccggctc acggtgagcc ggacggacaa
     3301 cggcgtggtc gtggtcacag gcctttcccg cgccgacgtg cagcgcacca tgaccatgct
     3361 gttcacggtg atcgccctgg cgaccctcgg cggcctgctg ctgctcgcgc tcaccacggc
     3421 cctcaccatc agcatggggc tccggccgct ccgcgccgtg gcggcgacgg ccacgcgggt
     3481 ggccgggcag cccctggacc gcggcgaggt gagcatcacg gagcgggtgc ccgactacga
     3541 ggccgacccg cgcaccgagg tgggccgcgt gggcgccgcg ctgaacaccc tgctggatca
     3601 cgtcgactcc tcactggcgg cgcgccagcg caacgaggag cggatgcggc gcttcgtcgc
     3661 cgacgcgagc cacgagctgc gcacgcccct ggcatccatc cgcggttact ccgagctgtc
     3721 gctgaaggcg atcaggcagg cggagcaggc ggacgccgat cagagcgcgc ggacgctgga
     3781 gaacaccacc tcggcgctgg agcgcatcca ggcccagtcg ctgcgcatga cgcggctggt
     3841 cgaggatctg ctgctgctgg cccgcctgga cgagggccgc gagctcgtga gcagggaggt
     3901 cgacctcgcc cagctcgccg tggaggcgct caccgatgcg cagccgacgg cgcccgacca
     3961 cgcctgggag ctggacgtcc ccgaggaccc ggtcatgctc atgggcgacg cgggacggct
     4021 gcaccaggtg ctcgcgaacc tgctcgccaa cgccaggacg cacacccccg cgggcaccac
     4081 gatcacgctg tccctggcga tcgagtccgg aaccgccgag ctgcgcgtgc acgacgacgg
     4141 ccccggcatc gacccgcagg tgcgggacga gctgttcgcc cgattcgcgc gcggcgatgc
     4201 gtcacgcgcc cgtcagaccg gcggcaccgg gctcgggctg gcgatcgcca aggcgatcgt
     4261 ggagggccac ggcgggacga tcgacgtgac gagcgcaccc ggcgacacga ccttcaccgt
     4321 gcgcctaccg ctggagaccg ctccgcagca gaccgtcccc gaggagttcc cccctccgcc
     4381 tcctcccgct cccccggctc agtagccggc gaaggcgtcc gtcgtgagac tgcgcgcctt
     4441 ctgcagagcg ggcgcgaggt ccgcgatcag gcgcggcggt cccgagatga acgcgtgccg
     4501 tgcggcgatg tcgggcacca cgcgctccaa gcccgcggca tccagccgca cgccctgcgc
     4561 ccaggtccag ttctcggcca gatccgccgg ctcgtcgcgc gtgaagacca cggcccgaac
     4621 accggtggcc tggagctcgt cgcggaacgc cagctcggcc gcctcggcgg ccacgtagac
     4681 gagcacgacg tcgcgctgca cgcccagcga ctgcatctgg cgcagctgcg agacgaacgg
     4741 cgtgacgccg atgcccgccg ccaccatgag ggtcggggcc cctccgcgcg gcaggaggaa
     4801 gtcgccccag gtcccggtca ccgccagcac cgccccgggc tccgccgccg cgagagcgcg
     4861 cttgtagctg gagggatgct tctgatcgcc gttcttgtac gcgatgcgca gtatgggcag
     4921 atcggcgggg gcggagacga tgctgaactc gcgccgggtg ccacgggcat ccggccggtg
     4981 gtgcggcaca tccagctcca ggtactgccc cggcaggaag ctcagccgcc ccttggtgcg
     5041 gaacgtcagt tcctgggcgg tgggcgtcac catccgcctc ttctccagga ccagccgcac
     5101 cgagccgcgc agcgcgaagg cgaacgccag caggttgccg accagcaggg cgcgctcctg
     5161 acccagggtg aacaggtcac cgatcgggat cggccagccc gccagcacgc ccacgagggc
     5221 tgcgacgctg aactgctgcc agcgccgcgg aggctgcgtg agcggctcgg agagcatgaa
     5281 ggcgccgagg aacaggtacg gcgtgtgcac gatggcgaac gccacggcat ccgcgatcgc
     5341 cacctcggtc cccgccgact gcgcgagcag cgcctgacgc accaccgaga cgccgacgac
     5401 gacgagcagg aagaccatca cgatgcgcac cttctcgatg cgccacagca cgagcagccc
     5461 cagcaccgcc accgcgacgg cgagcacggg cgttcccacc caccacgacg agcgggagcc
     5521 gaggccgagc accgtcacga cagccgcgcc gaaggccgca ggattcagca cgtgccggcc
     5581 gcgccacgcg atcaggtact tcgacaggct cgcgagggcg ccggccagcg cattgcccag
     5641 caggcctccg gccgtcagcg agggctgcat cacgaacagc aggatgagcg cggtgaccag
     5701 cgatgactcc acgcgccagg gcagccggag gatgcgctgc gctgcggcgt cgaccgccga
     5761 gatcacgatg gcgagcaccg cgaaggacgc caggatctcc gcggcgctcg gaacgacgag
     5821 cccggtgaag gagaagccga gggccaccac ggcgagcgcg acgagggcga agaacacgag
     5881 gcggtacatc gagatgccgc cgagcaccgc cagcacgcgc tgccgcagtg cggtcaggga
     5941 ggggatcact gcgtgacggc gtgcctcagg cggcgccgtt gaacatgctc gtgaccgaac
     6001 cgtcctcgaa gacctggcgg atggccgtgg ccagcagcgg tgcgatcggg aggatgacga
     6061 gctcgtccca gcgacgcgac tccgacagcg ggatcgtgtc ggtcacgacg acctggtcga
     6121 tcgcctcgtc ctgcaggcgc acggatgccg ggtcactgaa gatcgcatgc gtggcggcga
     6181 cgatcacctt gcgggcaccg ttcgccttca gcgcctgcgc ggccttcacg atcgtgccac
     6241 cggtgtcgat catgtcgtcg acgagcaggc aggtgcggtc gttcacggta ccgacgatct
     6301 cgtgcaccga gacctggttc gcgaccttcg ggtcgcggcg cttgtggatg atcgccagcg
     6361 gcgcgttcag gctgtcggac caggtgtcgg cgacgcgcac gcggcccatg tcgggcgaca
     6421 cgatggtgag cgtctcgcgg tcctcggcgg tgagggtctc ctcgaagtgc ttcagcagca
     6481 cgggcttggc gaagaggtgg tcgacgggac cgtcgaagaa gccctggatc tgcgcggcgt
     6541 gcagatccac gctcatgaca cggtcggcgc cggcgacctt cagcatgtcg gcgaccagtc
     6601 gggcgctgat cggctcgcga ccgcggccct tcttgtcctg gcgggagtac gggtagtacg
     6661 gggcgacgac ggtgatgcgc ttggcggatg cccgcttggc ggcgtccagc atgatcagcg
     6721 tctccatgag ccactcgttg accggctcgc cgaaggactg cacgatgaag aggtcgcagc
     6781 cgcggatcga cacctcgaag cgggcgtaga tctcacccga ggcgaacgtg cggtgctcga
     6841 cgggcgccat ctcctgaccc agcgcagcgg cgaccgccgc cgtcagctcg ggatgggagc
     6901 gaccgccggc gacgaccagt cgcttcttgg tcttggcgat cagcccgggt gcgaccccgt
     6961 tctcccgatc cagtgccacc gtcttcttct tgtgccccat ccgagccgcc tattccgccg
     7021 atcgttcccg tgcggcggca tccgccgcgc cggttcctgc tctgttcttc tcgacccacc
     7081 cctcgatgtt gcgctgaggg gcgacgctca tggccagcga gcctgcgggg acgtccttgc
     7141 ggacgacagc acctgcaccg gtcttcgcac ctgcaccaag cctaacgggc gcgacgagca
     7201 ccgtgtgaga gccggtgtgc acctcgtccc cgatctcggt gcggtgcttg ttcacatcgt
     7261 cgtagttcgc ggtgatcgtg ctcgcaccca ggttgacgtg gcggccgatc acggcatccc
     7321 ccacgtagga cagatgcggg accttgctgc cctcgccgat ctgcgcgttc ttggtctcga
     7381 cgtaggcgcc gatcttgccg ccctcgccga gcacggtccc gggtcgcagg aacgagaacg
     7441 ggcccaccgt ggcgcgagcg ccgatcacgg ccaggttcgc atcggtgcgc ttgacgacgg
     7501 cgtgctcgcc gacctcggtg tcgaccagtg tggtgtcggg gccgatggtg gcgcctgtcg
     7561 cgatcacgca ggcgcgcagg agctgcgtgt tcggcaggac ggtcacgtcg ggtgcgagca
     7621 cgacatcgtc gtcgatccac gtggtggccg ggtcgatgat cgtgacgccc tcgcgctgcc
     7681 agcgacggac gatgcgctgg ttgagcaccc ggccggcctc ggcgagctgg gcgcggtcgt
     7741 tcacgccgaa ggtggatgcc gcgtccggtg cgagctcggc ggcgacgtgc tcggccgcgc
     7801 cccgcagcag gccgatcacg tcggtgaggt agagctcgcc ctgagcgttg ttctggtcga
     7861 tgcgggtcag gaaggtgcgc agggccgcga cccggaagac gtagacgccc gcgttgatct
     7921 cggtgacggc ggcctcggcg tccgtggcgt ccttctgctc gacgatgcgc tcgacggcgc
     7981 cgtcggcgcc gcggatcacg cggccgtagc cggtcgggtc atccagatgg gcgctcagca
     8041 gggtcgccgc ggcggacgcc gcccggtgac cgtcgagcag gtgccgcagc gtgtcggcct
     8101 ccagcagcgg cacgtctccg gagagcacga gcacgtcgcc gtcgaagtcg tccgggagtg
     8161 cgtcgagtgc aacctgcacg gcgcggccgg tgcccgggat ctcgtcctgg tcgacgatgc
     8221 gcgcgtccgg gtactgctcg gcgagcgtcg cgatgacctg ctcccgctcg tgccgcacga
     8281 cgacctcgat gtgctgcgcc cccagcgcgt gcgcggtggt cagcacgtgc ccgaccaggg
     8341 gcctgccgcc gatctcgtgc agcaccttgg gcaggcgcga cttcatgcgg gtgccctgcc
     8401 ctgcggcgag gacgatgatg gcgagtgcgt tctccgacat gctccgccgc caggactcga
     8461 acctggactt cacagctcca aaggctgtcg tgctgccatt acaccacggc ggaccacggc
     8521 atcctccgac gggagccgcc cgtcaagtct gccatgagcg acggagtgcg acctgacagg
     8581 accgcactcc gtcgcgggcg tcagtgcgac accggcagcg atggcgcgag gcgtcgcaga
     8641 cggtggaacg tcggcagcag cgcgtcgtag atctcgtcga acaccggaag caacgagcgg
     8701 tacaccgcgg actcggcagg gcggggtcgc acgacgcgct cgaccggctg cagctgggcc
     8761 gcgacgtcga acgactcgat gagaccgagc gcctgcatgc cgagcagcgc cgcgccgaag
     8821 ccggatccct cgtgacctgc gagcagtccg atgggcgcgc cgaaggcgtc ggcgatcgtc
     8881 tgctgccaga gcggatgctt gatcaccccg ccggtggcgc ggatctcgtc gatctcgagg
     8941 cccgccgccc gcatcgactg cagcacgagg gcgagctgga gggcgacgcc ttcgacggcc
     9001 gccctgacca tgtggtcgcg ggtgtgcgca cgggtcaggc cgatgtacgc accgctggcg
     9061 atgctgctcc agtgcggggc gcgctcgctg agcaggtacg gcagcatcag caggccgccg
     9121 gatccgaccg gcaccgcccc tgcgcggtcc agaagctcct cgatcggcgt cgtggcgtcc
     9181 gcgatctcct gacgcaccca gtccagcgtg acgccgccgt tgttgatcgc gccgccgatc
     9241 acccagcggt cctcggtgag ggcgtagcag aacacgccgc cgcgcgggtc gaccaccggc
     9301 cggtccaccg cgac
//
LOCUS       DATMSX010000203        12948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_126275,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000203 DATMSX010000000
VERSION     DATMSX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126275"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VN041_06335"
     CDS             complement(<1..142)
                     /locus_tag="VN041_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HWU28699.1"
                     /translation="MSTEYRGVVVIGDALIDEIRDASGVRELVGGAALNVAVGLRRLG
                     VPA"
     gene            259..867
                     /locus_tag="VN041_06340"
     CDS             259..867
                     /locus_tag="VN041_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28700.1"
                     /translation="MKLRDGAAAALILAGVVILAACTPSADDATPSGSAKPTASASSS
                     PGPTKEPSTKPTPAPTSTVKAIALPKDCKAILSEQVLAQLEDVPLNDPAFGESGTLKN
                     GTLVCIWGSPQADTTGLTTRIERMPRGKALDMLNALADTEGYSCFTPSGGTRCEKTWQ
                     NEKYPVTDGRTLFWRDDVMIDTRYSNLAPKGYTSAIIKAIWG"
     gene            complement(1056..4922)
                     /gene="rpoC"
                     /locus_tag="VN041_06345"
     CDS             complement(1056..4922)
                     /gene="rpoC"
                     /locus_tag="VN041_06345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWU28701.1"
                     /translation="MLDATTFDELRIGLATADDIRGWSYGEVKKPETINYRTLKPEKD
                     GLFGEQIFGPSRDWECACGKYKRVRFKGIVCERCGVEVTKSSVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDMAPKDLEKVIYFAAYMVISVDEDARHRDLATQENNIRLELK
                     TLGDRRDAKIAERMAKLEEELAALEAEGAKADAKKKVKDAAEKEMALTRKGADEAIAK
                     LERVWEDFRTLEVGALRPEDDVFHELQDRFGQYFEAHMGAESIKRRLETFDLQGEAEN
                     LRLQISEGKGQRKIRAIKRLKVVSSFLETGMSPAAMVLDVVPVIPPELRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLRRLIDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GTGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIIVGPQLKLHQCGLPKQMALE
                     LFKPFVIKRLIDLGHSQNIKAAKRAVERTRPEVWDVLEEIIRERPVLLNRAPTLHRLG
                     IQAFEPQLVEGKAIQLHPLVCAAFNADFDGDQMAVHLPLSVEAQAEARVLMLASNNIL
                     KPSDGRPVTLPSQDMIIGLHHLTTVKEGAAGEGRAFGSVGEAILANDEGTLDLQAKVR
                     IRIPGLTFLEGEAPEGYERHGLVDASLGQAIFNDALPKGYPFVRGVADKGKLSQIVNK
                     LAEEYPKVETAASLDRIKDAGFHWATRSGVTVALSDVVTPPNKGEIVAGYEKQAEKVQ
                     GQYDRGLITDAERRRELIQIWTSATDDVQAAMMANFPEDNTINRMVTSGARGNWLQIR
                     NIAGMRGLVNNPKGELIPRPIISSYREGLSVAEYFIATHGTRKGLADTALRTADSGYL
                     TRRLVDVSQDVIIREDDCGTSKGLELPIAAPNSQGVLVRDANVENSVFARTLASEVVN
                     EAGEVLATAGEDVGDVLIDKLVAAGVETIKVRSVLTCDSAVGVCAQCYGRSLATGKTV
                     DIGEAVGIIAAQSIGEPGTQLTMRTFHLASAGDITQGLPRVQELFEARTPKGASPIAE
                     ADGRITIEENEKAKKVILTPDNGDEEVVYPVLKRATLLVEDGQHVTVGQPLQVGTLDP
                     KEVMRVMGAREVQRYLVGGVQGVYRSQGVPIHDKHIEVIVRQMLRKVTVVDHGDTTLL
                     PGEMVDLKRYQQINREAVSEGKRPASGRPELMGITKASLATESWLSAASFQETTRVLT
                     QAAMEGKRDPLVGLKENVIIGKLIPAGTGLSKYRDITVEATEEAKSERYPNRIFASDG
                     AYADGDFGYVDFDAFSTDDITPGTYN"
     gene            complement(4970..8470)
                     /locus_tag="VN041_06350"
     CDS             complement(4970..8470)
                     /locus_tag="VN041_06350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWU28702.1"
                     /translation="MAAARNASTSTTKKNGRGVSRLSFAKISDTLTVPDLLALQTESF
                     GWLVGNDDWKARVAEAKKAGRSDVPEVSGLGEIFEEISPIEDLGETMQLSFTNPYLEP
                     EKYSIEECKERGKTYAAPLYVEAEFMNHLTGEIKTQTVFMGDFPLQTDKGTFIINGSE
                     RVVVSQLVRSPGVYFDKTPDKTSDKDIVTARVIPSRGAWLEFEIDKRDQVGVRVDRKR
                     KQSVTVFLKALGMTSEEILAEFAGFPSIEETLAKDTILTKEDALRDIYRKLRPGEQVA
                     AEAARALLDNFYFNPKRYDLAKVGRYKINQKLGLAGSLTDSVLTVEDIVATIKYLVRL
                     HRGDESFEGIRSGKKAEIRIAVDDIDNFGNRRIRAVGELIQNQVRTGLSRMERVVRER
                     MTTQDIEAITPQTLINVRPVVAAIKEFFGTSQLSQFMDQNNPLAGLTNKRRLSALGPG
                     GLSRDRAGVEVRDVHPSHYGRMCPIETPEGPNIGLIGALATFARINSFGFIETPYRKV
                     EDGRVTDQIDYLTAAEEADFNIAQANAPLTKDGRFTESHVLARPRGGSGEVDLFLPEE
                     IGYIDVSPRQMVSVATSLVPFLEHDDAQRALMGANMQRQAVPLLRSDSPLVGTGMEGY
                     TAIDAGDVITALKAGVVSEVSADRVVVMLDEGGTQEYHLRKFDRSNQGTSYNQRVIVN
                     AGERVEVGEVIADGPATDNGELALGKNLLVAFMPWEGHNFEDAIILSQDLVKEDTLSS
                     IHIEEYEVDARDTKLGKEEITRDLPNVSPELLKDLDERGIIRIGAEVRPGDILVGKVT
                     PKGETELSAEERLLRAIFNEKSREVRDTSLKVPHGEQGTIIAVKEFNAEEGDDELGSG
                     VNRRVVVYIAQKRKITEGDKLAGRHGNKGVIAKILPVEDMPFLSDGTPVDVVLNPLGI
                     PGRMNFGQVLETHLGWIAKTGWKVEGNPEWAVKLADDARDVAPNTKVATPVFDGASEE
                     EIAGLLDSTLETRDGVRLIDRSGKTQLFDGRSGDPFPAPVSVGYMYILKLHHLVDDKI
                     HARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDILGR
                     VKVYEAIVKGENIQEPGIPESFKVLMKEMQSLCLNVEVLSADGTAVNLRDTDDEAFRA
                     AEELGINISSRFEAASIDEI"
     gene            8710..9936
                     /locus_tag="VN041_06355"
     CDS             8710..9936
                     /locus_tag="VN041_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWU28703.1"
                     /translation="MKRTPMAQHAPNRTPTAWATLLRLLGIGLAVVTVSAVAVAGYVV
                     VDLTQRAGADAVKLEAAPTVPPPSLGEFEGPFSMLLIGTDECGEVSKQVLGERCRRED
                     GILNDINLLVHVSAAPRHVTVVSLPRDLMLKVPDCRREDGSTASGMRKAPINTVYSHA
                     GLSCVAKTVSELSGMDIGFAAKLSFDGVMKITDAIGGVEVCIGPKGLHDKKYTGIDWP
                     PGPRTIKGLEALQFIRVRHGIGNNSDLSRISNQQQYMSRLARTILSSETLTDVPKMLR
                     LASTIVDNVQASDELADPVRLAQLALTMKGVRYDDFLFLQLPTLDDPDAPTRRVVENT
                     DAAKPMWEAIRTGGSMQITGAASNGGSATVETPATPPPSTDPSPTPSSTPPDTTVLPS
                     EITGIDLNQQTCTGSH"
     gene            9959..10828
                     /locus_tag="VN041_06360"
     CDS             9959..10828
                     /locus_tag="VN041_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWU28704.1"
                     /translation="MGRARGKDAENPRARLEHGGTAEIAPSEFTSGFELIVDGTPQSH
                     VDLEDPTHLHFEYIVRMGAVIDQLGPSASAPLSVVHLGAGALTIPRYIDATRPGSRQQ
                     VIEWEAPLVALVREHLPLPRGAAVRIRIGDAREGLKRLPPALHGQCDLVVSDVFSGAQ
                     TPAHLTSVEFYREIAELLAPGGVLLVNVADGPGLAFARRQVATAATVFPEVALLADTQ
                     VLKGRRFGNLVLAASALPLPTDWLPRMLAAGPHPAKIAQGEEVAAFARGASIVTDADA
                     VASPRPDSSLFLR"
     gene            10924..11454
                     /locus_tag="VN041_06365"
     CDS             10924..11454
                     /locus_tag="VN041_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28705.1"
                     /translation="MNLIQGYDPDTLREIVSERECLMRLAEIENQRSLPVLLERVWLL
                     KVLDRLDEALETAEETVRQARMAGTRKDLLRARVLHATVLQHRGAHAAAALELAHSAS
                     EAEGQGWIGIAAFAHQHHGKNAYEARDFEQARESFKRSLFLRREAGADDAELEQALRA
                     IEAAERRSAEGADALV"
     gene            11542..12030
                     /locus_tag="VN041_06370"
     CDS             11542..12030
                     /locus_tag="VN041_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HWU28706.1"
                     /translation="MADLNRVRVWAQALIDMHLDDSWSFDFDHAKRRAGLCDYRRKRI
                     TVSRYLAARFEDDEIHQTLLHEVAHAVAGHTAAHGPEWKRVARDLGYVGGTTHHGETA
                     TELAPWVGTCPVGHVTYRHRRPARETSCARCSRSFDSRYLFAWTRREITAADRRAAQL
                     PR"
     gene            complement(12109..12483)
                     /locus_tag="VN041_06375"
     CDS             complement(12109..12483)
                     /locus_tag="VN041_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HWU28707.1"
                     /translation="MRYLPAFVLDAVLVLIFSVIGRASHGEEPIGFLITAWPFLVALV
                     IGHLLAMLVPARPRRPWSLGWGVVAWIVTVAGGMLLRIATGDTAQAPFIIVATVVLGV
                     FLLGWRLIALLVRRYRGPREQA"
     gene            complement(12557..>12948)
                     /locus_tag="VN041_06380"
     CDS             complement(12557..>12948)
                     /locus_tag="VN041_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphosulfolactate phosphohydrolase"
                     /protein_id="HWU28708.1"
                     /translation="AVMTLQERRAERTSVTVIAAGERTPAGDLRFAVEDQLGAGAVIA
                     ALTDLGIDHSSPDAAVAAEAFRALRRALRHLLSASGSGRELVDGVASTARMVEAGIRP
                     ATTEEAAEPDAVDAVPMLRDGSFERFV"
BASE COUNT         2044 a         4371 c         4406 g         2127 t
ORIGIN      
        1 tcgccggcac gccgagccgg cgcagcccga cggcgacgtt caaggcggca ccgccgacca
       61 gctcgcggac gcccgaagcg tcccggatct cgtcgatcag cgcgtcgccg atgacgacga
      121 ccccacggta ttcagtgctc acgtggcgag actaccgcgc gaggcagccc tgcatccgcc
      181 ggatgaacgg catccggtcg cgcgcaccgg acggcgcggc gcgctccccc gggcttcctc
      241 cggtcctact ctgtgggcat gaagctccgc gacggcgcgg cggccgcgct catcctcgca
      301 ggtgtcgtga tcctcgcggc ctgcacgccc tccgctgacg acgcgacgcc gtccggcagc
      361 gcgaagccaa cggcatccgc ctcctcgtcc ccggggccga cgaaagagcc gtcgacgaag
      421 ccgacgcccg ctcccacctc gaccgtgaaa gccatcgcgc tgccgaagga ctgcaaggcg
      481 atcctctccg agcaggtgct cgcgcagctc gaggacgtgc cactgaacga ccccgccttc
      541 ggcgagagcg gcactctcaa gaacggaacg ctggtgtgca tctggggctc accgcaggcc
      601 gacaccaccg gtctcacgac ccggatcgag cggatgcccc gcggcaaggc actcgacatg
      661 ctcaacgccc tcgcggacac cgaggggtac tcctgcttca cgccctcggg cggcacccgc
      721 tgcgagaaga cctggcagaa cgagaagtac ccggtcacgg acggccgcac cctgttctgg
      781 cgcgacgacg tgatgatcga cacgcgctac tcgaacctcg caccgaaggg ctacacctcc
      841 gcgatcatca aggcgatctg gggctgagca tcatcacccc tccgcccacc gtgtacagat
      901 gtcggcggaa agcacggatg tcggtgcgga tgccgggttt tcgacagaca tccgtgatct
      961 ggaccgacat ctgtacctgg tggcccggct tctgccgcgc atgcgaaagg ccccggccct
     1021 cctgagaggg gccggggcct tcaatcacgc ctcaatcagt tgtaggtgcc gggcgtgatg
     1081 tcgtcggtcg agaacgcgtc gaagtcgacg tagccgaagt cgccgtcggc gtacgcaccg
     1141 tcggatgcga agatccggtt cgggtagcgc tcgctcttgg cctcctcggt cgcctcgacc
     1201 gtgatgtcac ggtacttcga cagaccggtt ccggccggga tgagcttacc gatgatgacg
     1261 ttctccttga gaccgaccag cgggtcgcgc ttgccctcca tggcggcctg cgtgagcacg
     1321 cgggtcgtct cctggaagga cgcagccgac agccacgact cggtcgcgag ggacgccttg
     1381 gtgataccca tcagctccgg gcgaccggac gcggggcgct tgccctcgga cacggcctcg
     1441 cggttgatct gctggtagcg cttcaggtcg accatctcac cggggagcag cgtggtgtcg
     1501 ccgtggtcga ccacggtgac cttgcgcagc atctggcgca cgatgacctc gatgtgcttg
     1561 tcgtggatcg gcacaccctg cgagcggtac acgccctgca cgccgccgac gaggtacctc
     1621 tgcacctcgc gggcacccat gacacgcata acctccttgg ggtcgagcgt gccgacctgc
     1681 aggggctgac cgaccgtgac gtgctggccg tcctcgacga gaagcgtcgc acgcttcagc
     1741 accgggtaga ccacttcctc gtcaccgttg tcgggcgtga ggatgacctt cttggccttc
     1801 tcgttctcct cgatcgtgat gcggccgtcg gcctcggcga tcggggacgc gcccttgggc
     1861 gtacgagcct cgaagagctc ctgcacacgg ggcagaccct gggtgatgtc acccgcggat
     1921 gccaggtgga aggtacgcat cgtcagctgg gtaccgggct caccgatcga ctgggccgcg
     1981 atgatgccca cggcctcacc gatgtccacc gtcttgccgg tggccagcga acggccgtag
     2041 cactgcgcgc agacaccgac ggccgagtcg caggtcagca cggagcggac cttgatcgtc
     2101 tcgacaccgg ccgcgacgag cttgtcgatg agcacgtcgc cgacgtcctc accggcggtg
     2161 gcgagaacct cgccggcctc gttgacgacc tcggatgcca gggtgcgggc gaacacggag
     2221 ttctccacgt tcgcgtcgcg caccagcacg ccctgcgagt tgggagcggc gatcggcagc
     2281 tccagaccct tcgacgtgcc gcagtcgtcc tcacggatga tgacgtcctg cgagacgtcc
     2341 accagacgac gggtcaggta acccgagtcg gcggtgcgca gagcggtgtc ggccagaccc
     2401 ttgcgggtac cgtgcgtcgc gatgaagtac tccgccaccg acagaccctc gcggtacgag
     2461 gagatgatcg gacgcgggat gagctcaccc ttcgggttgt tcaccagacc gcgcataccg
     2521 gcgatgttac ggatctgcag ccagttacca cgggcgcccg aggtcaccat gcggttgatg
     2581 gtgttgtcct ccgggaagtt cgccatcatg gcggcctgga catcgtcggt cgccgacgtc
     2641 cagatctgga tcagctcgcg acgacgctca gcatccgtga tcagaccacg gtcgtactga
     2701 ccctggacct tctcggcctg cttctcgtag cccgcgacga tctcgccctt gttcggcggg
     2761 gtgacgacgt cactgagcgc gacggtcaca ccggaacggg tcgcccagtg gaaaccggca
     2821 tccttgatgc ggtccagcga cgcggcggtc tcgaccttgg ggtactcctc ggcgagcttg
     2881 ttgacgatct gcgacagctt gcccttgtcg gcgacgccgc gcacgaacgg gtagcccttc
     2941 ggcagggcgt cgttgaagat cgcctggccg agcgaggcat ccacgaggcc gtggcgctcg
     3001 tagccctcgg gggcctcgcc ctcgaggaag gtcagacccg ggatgcggat gcggaccttc
     3061 gcctgcaggt cgagggtgcc ctcgtcgttg gcgaggatcg cctcgcccac cgagccgaac
     3121 gcacgaccct caccggcagc gccctccttg accgtggtca ggtggtgcag accgatgatc
     3181 atgtcctgcg agggcagggt gaccggacga ccgtccgacg gcttgaggat gttgttcgag
     3241 gcgagcatca gcacgcgggc ctcggcctgg gcctcgaccg acagcggcag gtggactgcc
     3301 atctggtcac cgtcgaagtc ggcgttgaac gccgcgcaca ccagcgggtg cagctggatg
     3361 gccttgccct cgacgagctg cggctcgaag gcctggatgc ccaggcggtg cagggtgggg
     3421 gcgcggttca gcagcacggg acgctcgcgg atgatctcct cgagcacgtc ccacacctcg
     3481 ggacgggtgc gctcgacggc acgcttggcg gccttgatgt tctgcgagtg accgaggtcg
     3541 atcaggcgct tgatcacgaa cggcttgaac agctccagcg ccatctgctt gggcagaccg
     3601 cactggtgca gcttcagctg cggaccgacg atgatgaccg aacggcccga gtagtccacg
     3661 cgcttgccga gcaggttctg gcggaagcga ccctgcttgc ccttcagcat gtcgctgagg
     3721 gacttcaggg cacggttgcc ggtaccggtg acgggacgac cacggcggcc gttgtcgaac
     3781 agcgcgtcga cggcctcctg cagcatccgc ttctcgttgt tgacgatgat ctcgggggcg
     3841 ccgaggtcga tcagacgacg aagacggttg ttgcggttga tcacgcgacg gtacaggtcg
     3901 ttcaggtcgg aggtcgcgaa gcggccaccg tctagctgca ccatcggacg cagctccggc
     3961 gggatcaccg ggacgacgtc cagcaccatg gcggccgggc tcatgccggt ctccaggaag
     4021 gagctcacga ccttcaggcg cttgatcgca cggatcttgc gctggccctt gccctcggag
     4081 atctgcagac gcaggttctc ggcctcgccc tgcaggtcga aggtctccag gcggcgcttg
     4141 atcgactcgg cacccatgtg ggcctcgaag tactgaccga agcggtcctg cagctcgtgg
     4201 aacacgtcgt cctcggggcg cagggcgccc acttcgagcg tgcggaagtc ctcccacacg
     4261 cgctcgagct tggcgatggc ctcgtcggca cccttgcggg tgagggccat ctccttctcg
     4321 gcggcgtcct tgaccttctt cttggcgtcg gccttggcac cctccgcctc cagggcggcg
     4381 agctcctcct ccagcttggc catgcgctcc gcgatcttgg cgtcgcggcg gtcgcccagc
     4441 gtcttcagct cgaggcggat gttgttctcc tgcgtggcga ggtcgcggtg acgagcatcc
     4501 tcgtcgaccg agatcaccat gtaggcggcg aagtagatga ccttctcgag gtccttcggc
     4561 gccatgtcga gcaggtagcc caggcgcgac ggcacgccct tgaagtacca gatgtgcgtc
     4621 acgggcgcgg cgagctcgat gtggcccatg cgctcacggc ggacggagga cttggtgacc
     4681 tccacgccgc agcgctcgca cacgatgccc ttgaagcgga cgcgcttgta cttgccgcag
     4741 gcgcactccc agtcgcggga gggtccgaag atctgctcgc cgaagaggcc gtccttctcg
     4801 ggcttcagcg tgcggtagtt gatcgtctcg ggcttcttga cctcgccgta ggaccatccg
     4861 cggatgtcgt cagcggtcgc gaggccgatt cgaagctcat cgaaagttgt tgcgtcgagc
     4921 acttgttctc ctgtgtagaa aaaatctgtc gcttgtcgaa ggtccgagat cagatctcgt
     4981 cgatcgatgc ggcttcgaaa cggctggaga tgttgatgcc gagctcctcc gccgcgcgga
     5041 aggcctcgtc atcggtgtcg cggaggttga ccgcggtgcc gtcggccgag agcacctcga
     5101 cgttcaggca gagcgactgc atctccttca tgagcacctt gaaggactcg gggatgccgg
     5161 gctcctggat gttctcgccc ttgacgatcg cctcgtacac cttgacgcgg ccgaggatgt
     5221 cgtcggactt gatcgtgagg agctcctgca gcgcgtacgc ggcaccgtag gcctcgaggg
     5281 cccacacctc catctcaccg aagcgctgtc caccgaactg cgccttacca ccgagcggct
     5341 gctgggtgat catcgagtac gggcccgtgg agcgcgcgtg gatcttgtcg tccacgaggt
     5401 ggtgcagctt caggatgtac atgtatccga ccgagacggg cgccgggaac gggtcgccgg
     5461 agcggccgtc gaacagctgg gtcttgccgg agcggtcgat caggcggacg ccgtcacggg
     5521 tctcgagcgt cgagtccagg agacctgcga tctcctcctc gctggcaccg tcgaacaccg
     5581 gggtcgcgac cttggtgttc ggggcgacgt cgcgggcgtc gtcagcgagc ttgacggccc
     5641 actcggggtt gccctcgacc ttccagcccg tcttggcgat ccatccgagg tgggtctcga
     5701 gcacctggcc gaagttcatt cgacccggga tgccgagcgg gttcagcacc acgtcgaccg
     5761 gcgttccgtc ggagaggaac ggcatgtcct cgaccggcag gatcttcgcg atgacgccct
     5821 tgttgccgtg gcggccggcg agcttgtcgc cctcggtgat cttgcgcttc tgggcgatgt
     5881 agaccacgac gcggcggttg acgcccgagc ccagctcgtc gtcgccctcc tcggcgttga
     5941 actccttgac ggcgatgatc gtgccctgct cgccgtgcgg caccttcagg gaggtgtcac
     6001 ggacctcgcg gctcttctcg ttgaagatcg cgcggagcag gcgctcctcg gccgacagct
     6061 cggtctcgcc cttcggcgtg accttgccga ccaggatgtc gccggggcgg acctcggcgc
     6121 cgatgcggat gatgccgcgc tcgtcgaggt ccttcagcag ctcagggctg acgttgggga
     6181 ggtcacgggt gatctcctcc ttgcccagct tcgtgtcgcg ggcgtcgacc tcgtactcct
     6241 cgatgtggat cgacgagagg gtgtcctcct tcaccagatc ctggctcagg atgatggcgt
     6301 cctcgaagtt gtggccctcc cacggcatga acgcgacgag caggttcttg ccgagcgcga
     6361 gctcgccgtt gtcggtggcg ggaccgtcgg cgatgacctc gccgacctcg acgcgctcac
     6421 cggcgttgac gatcacgcgc tggttgtagg acgtgccctg gttcgagcgg tcgaacttgc
     6481 gcaggtggta ctcctgcgtg ccgccctcgt cgagcatgac gacgacgcgg tccgcggaga
     6541 cctcgctgac gacaccggcc ttcagcgcgg tgatcacgtc accggcgtcg atcgcggtgt
     6601 agccctccat accggtgccg acgagcggcg agtcggaacg gagcagcggc acggcctgac
     6661 gctgcatgtt ggcacccatg agggcgcgct gcgcgtcgtc gtgctcgagg aacggcacca
     6721 gcgaggtcgc gaccgacacc atctggcgcg gcgagacgtc gatgtagccg atctcctcgg
     6781 gcaggaacag gtcgacctca cccgatccgc cgcgggggcg ggccagcacg tggctctcgg
     6841 tgaagcggcc gtccttggtg aggggcgcgt tcgcctgggc gatgttgaag tcggcctctt
     6901 cggcggcggt gaggtagtcg atctggtcgg tgacccggcc gtcctcgacc ttgcggtacg
     6961 gggtctcgat gaagccgaag gagttgatgc gcgcgaacgt ggcgagcgca ccgatcaggc
     7021 cgatgttcgg gccttcaggg gtctcgatcg ggcacatgcg gccgtagtgc gaggggtgga
     7081 cgtcacggac ctcgacgcca gcacggtcgc ggctcagacc accggggccg agggccgaga
     7141 ggcggcgctt gttggtcaga cccgcgagcg ggttgttctg gtccatgaac tgcgacagct
     7201 gcgaggtgcc gaagaactcc ttgatcgcgg ccacgacggg acgcacgttg atcagggtct
     7261 gcggggtgat cgcctcgatg tcctgcgtgg tcatgcgctc gcggacgacg cgctccatgc
     7321 gggacagacc ggtgcggacc tggttctgga tcagctcgcc caccgcgcgg atgcgacggt
     7381 tgccgaagtt gtcgatgtcg tcgaccgcga tgcggatctc ggccttcttg ccggagcgga
     7441 tgccctcgaa cgactcgtcg ccgcggtgca ggcgcaccag gtacttgatc gtcgcgacga
     7501 tgtcctcgac ggtcagcacc gagtcggtga gggaacctgc caggcccagc ttctggttga
     7561 tcttgtagcg acccaccttg gccaggtcgt agcgcttcgg gttgaagtag aagttgtcca
     7621 gcagcgcgcg cgcggcctcg gcggcgacct gctcgcccgg acggagcttg cggtagatgt
     7681 cgcggagcgc gtcctccttg gtgaggatgg tgtccttcgc gagcgtctcc tcgatggagg
     7741 ggaacccggc gaactcggcg aggatctcct cgctggtcat gccgagggcc ttcaggaaga
     7801 ccgtgaccga ctgcttgcgc ttgcggtcga cgcgcacgcc gacctggtcg cgcttgtcga
     7861 tctcgaactc gagccaggcg ccgcggctgg ggatgacgcg cgcggtgacg atgtccttgt
     7921 ccgaggtctt gtcgggggtc ttgtcgaagt agacacccgg cgagcggacg agctgcgaga
     7981 cgacgacgcg ctcggagccg ttgatgatga acgtgccctt gtcggtctgc agggggaagt
     8041 cgcccatgaa gaccgtctgg gtcttgatct cacccgtgag gtggttcatg aactcggcct
     8101 cgacgtacag cggggcggcg taggtcttgc cgcgctcctt gcactcctcg atcgagtact
     8161 tctcgggctc gaggtagggg ttcgtgaacg agagctgcat ggtctcgccg aggtcctcga
     8221 tcggcgagat ctcctcgaag atctcgccca ggccggacac ctcggggacg tcgctgcggc
     8281 cggccttctt ggcctctgcg acccgggcct tccagtcgtc gttcccgacg agccagccga
     8341 acgactccgt ctgcagtgcg agcagatcgg gaaccgtcag cgtgtcggag atcttggcga
     8401 acgagaggcg ggagactccg cggccgttct tcttggtggt ggatgtggat gcgttgcgag
     8461 cagcagccaa gggagtaacc tccgtgagcc cgggcagggg ctggtcgatt cctagacgtc
     8521 ggtggagtgc acgctcgaga actcaacaat tgccgaccac catatgaggg catggagaag
     8581 cgagcgcaac tacctactat aggcacgaat cggcgacatg gcaagctgaa tccttgacca
     8641 atcctgaaag ctgcggtatg agcgccgctc tcggtcgacg gtcgggagcc acaggtaggg
     8701 tcggatgcca tgaagcggac cccgatggcg cagcacgctc cgaatcgcac gccgacggcg
     8761 tgggccactc tgctgcgtct gctgggcatc ggactggccg tcgtgaccgt ctccgccgtg
     8821 gccgtcgccg gatacgtcgt ggtcgatctc acgcagcgcg cgggcgcgga cgccgtgaag
     8881 ctggaggcgg cgccgacggt gccgccgccg tcgctcggcg agttcgaggg gccgttctcg
     8941 atgctgctga tcggcacgga cgagtgcggc gaggtgtcga agcaggtgct cggcgagcgc
     9001 tgccgtcgtg aggacggcat cctgaacgac atcaacctcc tcgtgcacgt ctcggcggca
     9061 ccgcggcacg tgaccgtagt gtcgctcccc cgcgacctga tgctgaaggt gccggactgc
     9121 aggcgcgagg acggcagcac ggcatccgga atgcgcaagg cgcccatcaa caccgtctac
     9181 tcccacgccg ggctgtcatg cgtcgccaag acggtgtcgg aactgagcgg gatggacatc
     9241 ggcttcgccg cgaagctcag cttcgacggc gtgatgaaga tcacggatgc catcggcggc
     9301 gtcgaggtgt gcatcgggcc gaagggcctg cacgacaaga agtacaccgg catcgactgg
     9361 ccgccggggc cgaggacgat caagggattg gaagccctgc agttcatccg ggtgcggcac
     9421 gggatcggca acaacagcga cctgtcgcgc atctccaacc agcagcagta catgtcccgg
     9481 ctggcgcgca cgatcctcag cagcgagacg ctcaccgacg tccccaagat gctgcgcctc
     9541 gccagcacga tcgtggacaa cgtgcaggcg agcgacgagc tggccgaccc cgtcaggctg
     9601 gcgcagctcg cactgaccat gaagggcgtc cgctacgacg acttcctctt cctgcagctg
     9661 cccacgctcg acgacccgga tgcgccgact cggcgcgtcg tggagaacac cgacgcggcg
     9721 aagccgatgt gggaggcgat caggaccggc gggtccatgc agatcaccgg cgccgcgagc
     9781 aacggcggca gcgcgacggt cgagacaccg gccacgcctc cgccctcgac agacccctcc
     9841 cctactccgt cgtcgactcc cccggacacc accgtgctcc cctccgagat cacgggtatc
     9901 gacctcaacc agcagacctg caccggcagc cactgacgcg ctgggtaggc tcgttcccat
     9961 gggacgagca cgcgggaagg acgccgagaa cccgcgtgca cggctggagc acggcggcac
    10021 cgccgagatc gcgccgagcg agttcaccag cggcttcgag ctgatcgtcg acggcacgcc
    10081 gcagtctcac gtcgatctcg aggaccccac gcatctccac ttcgagtaca tcgtgcggat
    10141 gggcgccgtg atcgatcagc tcggaccttc ggcatctgct ccgctgagcg tcgtgcacct
    10201 gggcgcgggc gcgctcacca tcccccgcta catcgacgcg acccgaccgg gttcgcggca
    10261 gcaggtgatc gaatgggagg cgcccctggt cgcgctggtg cgagagcacc tgccgctgcc
    10321 gcgtggcgcg gccgtccgga tccgcatcgg cgacgcccgt gaggggctga agcggctccc
    10381 tcccgcgctg cacggccagt gcgatctggt ggtgtcggat gtcttctcgg gcgcgcagac
    10441 ccccgcgcat ctcaccagcg tggagttcta ccgcgagatc gccgagcttc tggcgcccgg
    10501 cggtgtgctg ctcgtgaacg tcgccgacgg acccggcctc gccttcgccc ggcggcaggt
    10561 cgccaccgcc gcgacggtgt tccccgaggt ggcgctgctc gccgatacgc aggtgctcaa
    10621 gggtcgccgg ttcgggaacc tcgtgctcgc ggcatccgct cttccactgc ccaccgactg
    10681 gctgccgcgg atgctggcgg ccggccccca ccccgccaag atcgcgcagg gcgaggaggt
    10741 cgcggcgttc gcccgtggcg cgtcgatcgt gacggatgcg gatgccgtgg cctcgccgcg
    10801 tccggactcg agcctcttcc tgcgctgaca tcctgtcccg gtccgctcct cgcagggcgt
    10861 gcctcgcccg gttcccaggt cggcgatgca gactggatgc tgcgcggaga ggagccagca
    10921 gtcgtgaatt tgatccaggg ttacgacccc gacacccttc gtgagatcgt cagcgagcgc
    10981 gagtgcctga tgcggcttgc cgagatcgag aaccagcgca gcctgccggt gctgctcgag
    11041 cgcgtgtggc tgctcaaggt gctcgatcgt ctcgacgagg ccctcgagac cgccgaggag
    11101 accgtgcgcc aggcgcggat ggcgggcacc cgcaaggatc tgcttcgggc gcgcgtgctg
    11161 cacgccaccg tgctgcagca tcgcggtgca catgccgcag ctgcgctgga gctcgcccac
    11221 agcgcgagtg aggcggaggg ccagggctgg atcggcatcg cggccttcgc ccatcagcac
    11281 cacggcaaga acgcctacga ggcccgcgac ttcgagcagg cccgggagag cttcaagcgc
    11341 tcgctgttcc tgcgtcgcga ggccggtgcc gacgacgccg agctggaaca ggcgctgcgc
    11401 gccatcgagg ccgccgagcg ccgcagcgcc gagggcgccg acgcactggt ctgaggtccg
    11461 gatcctgagc ccgtccgccc ccggctcgct gagcccgtcg aagcggagcc cgatgtcggc
    11521 cgacggcttt aatctgatgt catggccgat ctgaaccgcg tgcgagtctg ggcacaggcg
    11581 ctgatcgaca tgcacctcga cgactcgtgg agcttcgact tcgaccacgc caagcgccgc
    11641 gcggggctgt gcgactaccg gcgcaagcgc attaccgtgt cccgctacct ggccgcccgg
    11701 ttcgaggacg acgagatcca tcagacgctg ctgcacgagg tcgcgcacgc cgtcgcaggg
    11761 cacaccgcag cgcacggtcc ggagtggaag cgcgtcgcgc gggacctcgg ctatgtgggt
    11821 ggcaccaccc accacggtga gacggccacg gagctcgccc cctgggtggg cacgtgcccc
    11881 gtcggacacg tcacctaccg gcatcgtcgt cccgctcgcg agacgtcctg cgcgaggtgc
    11941 tcacgcagct tcgacagccg ctatctgttc gcgtggaccc gacgcgagat cacggccgcc
    12001 gatcggcgag ccgctcaact cccccgctga ccgatcgatc ccgcggcgta cgcgcccgaa
    12061 ctctgctccg ttcgacgtgc gccccacgcg gaaaggcact gcgccggatc aggcctgctc
    12121 gcgcgggccg cggtagcggc gcacgagcag cgcgatcagg cgccagccga gcaggaagac
    12181 acccagcacc acggtcgcca cgatgatgaa cggcgcctgc gccgtgtcgc ccgtggcgat
    12241 gcgcagcagc atcccgcccg ccaccgtcac gatccaggcg accactcccc agcccagcga
    12301 ccaggggcgc cgcgggcggg cgggcaccag catcgcgagc aggtgcccga tgaccagggc
    12361 gacgaggaac ggccacgcgg tgatgaggaa cccgatcggt tcctcaccgt gcgacgcgcg
    12421 cccgatgacg gagaagatca gcaccagcac ggcgtcgagc acgaaggcgg gaaggtacct
    12481 catgcaagaa ccctatgcgc atccgccggc ggctcggcgc ggcgcgcggc gcccaccggc
    12541 aaacacgaga atgccctcag acgaagcgct cgaaggagcc gtcgcgcagc atggggacgg
    12601 catccacggc atccggctca gcagcctcct ccgtggtcgc agggcggatg cccgcctcga
    12661 ccatccgcgc ggtcgaggcc actccgtcca ccagctcacg tcccgaccca ctggcgctga
    12721 gcaggtgccg gagcgcccgg cgcagcgcac ggaacgcctc ggctgccaca gccgcatcgg
    12781 gcgaggagtg atcgatgccg aggtcggtca gcgcggcgat cacggccccc gctccgagct
    12841 ggtcctcgac ggcgaagcgc aggtcgccgg cgggagtcct ttcccccgcg gcgatcacgg
    12901 tcaccgacgt gcgttcggcg cggcgctcct gcagcgtcat gacggcac
//
LOCUS       DATMSX010000204         9585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3053,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000204 DATMSX010000000
VERSION     DATMSX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VN041_06385"
     CDS             complement(<1..308)
                     /locus_tag="VN041_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWU28709.1"
                     /translation="MAIRKYKPTTPGRRGSSVADFAEITRSTPEKSLLRPLSKTGGRN
                     NQGRITTRHIGGGHKRQYRVIDFRRNDKDGVNAKVAHIEYDPNRTARIALLHYFDGEK
                     R"
     gene            complement(329..649)
                     /gene="rplW"
                     /locus_tag="VN041_06390"
     CDS             complement(329..649)
                     /gene="rplW"
                     /locus_tag="VN041_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWU28710.1"
                     /translation="MTEQNILATALNKDPRDIILGPVVSEKSYGLIDEGKYTFLVDPR
                     ASKTEIKLAIEKIFGVKVASVNTINRVGKARRTRFGTGKRKDTKRAIVALKSGSIDIF
                     TAVG"
     gene            complement(646..1308)
                     /gene="rplD"
                     /locus_tag="VN041_06395"
     CDS             complement(646..1308)
                     /gene="rplD"
                     /locus_tag="VN041_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWU28711.1"
                     /translation="MADSTLALDVIAVDGKKAGSIELPAAIFDVKTNVPLIHQVVVAQ
                     LAAARQGTHSTKRRGEVSGAGRKPFKQKGTGNARQGSIRAPHMTGGGIVHGPKPRDYS
                     QRTPKKMIAAALLGALSDRFRGERLHAVEAFVADATPSTKTAAGLLAAVAGPKNVLVV
                     IERSDELTVKSVRNLPNVHVLSFDQLNAYDVVVSDDIVFTKAALEAFIASKTGATEEV
                     SA"
     gene            complement(1311..1970)
                     /gene="rplC"
                     /locus_tag="VN041_06400"
     CDS             complement(1311..1970)
                     /gene="rplC"
                     /locus_tag="VN041_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWU28712.1"
                     /translation="MADINAKISKGMLGTKLGMTQVWNENGKLVPVTVIELAPNVVTQ
                     IRTPEKDGYNAVQIAYGQIDPRKVNKPLTAHFEAAGVTPRRHVTEIRTADAADYALGQ
                     ELTVDGTFEAGQLVDVVGTSKGKGTAGVMKRHNFKGVSASHGAHRNHRKPGSIGASST
                     PSRVFKGMRMAGRMGGERVTVLNLTVHAVDVEKGLLLVKGAVPGARGRIVFVRNAVKG
                     A"
     gene            complement(1979..2287)
                     /gene="rpsJ"
                     /locus_tag="VN041_06405"
     CDS             complement(1979..2287)
                     /gene="rpsJ"
                     /locus_tag="VN041_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWU28713.1"
                     /translation="MAGQKIRIRLKSYDHEVIDTSARKIVDTVTRAGATVVGPVPLPT
                     EKNVIAVIRSPHKYKDSREHFEMRTHKRLIDIVDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            3089..3754
                     /locus_tag="VN041_06410"
     CDS             3089..3754
                     /locus_tag="VN041_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28714.1"
                     /translation="MDDSLGTHTRDAAAIPRRTLIRAGFWSVPVIAAATAAPLAAAST
                     VGSLEIFATRSSTAMLPLQTLDGTSVQPEATTTLPIGVTIWNRAGSTLADSGATLTLA
                     CDLPDYTLASGIARGVAVYSFAGVVIPSSARSIVYAEDGAGTPAGFPRTSFTTALPLD
                     ISAGTFSYDFELECRLTNETDSPTIDSGLNFPFRMTLRLSDGTVAAENSQPLYFGPGA
                     NVL"
     gene            complement(3827..4039)
                     /locus_tag="VN041_06415"
     CDS             complement(3827..4039)
                     /locus_tag="VN041_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28715.1"
                     /translation="MGIEDLVNQGKELFEQNKEKVEELLHSEQAEGISDKVLDGAAEL
                     AKKVAPGAAEQIDGVRDNVDGAVGNE"
     gene            complement(4214..5407)
                     /gene="tuf"
                     /locus_tag="VN041_06420"
     CDS             complement(4214..5407)
                     /gene="tuf"
                     /locus_tag="VN041_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWU28716.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLSAAISKVLADTYPSET
                     NVQRDFASIDSAPEERQRGITINISHIEYETPKRHYAHVDAPGHADYVKNMITGAAQM
                     DGAILVVAATDGPMAQTREHVLLAKQVGVPYLLVALNKSDMVDDEEILELVELEVREL
                     LSSQGFDGDNAPVVQVSALKALEGDEKWVKAILELMQAVDDSVPDPERDRDKPFLMPI
                     EDVFTITGRGTVVTGRAERGTLAINSEVEIVGIRPTQKTTVTGIEMFHKQLDEAWAGE
                     NCGLLLRGTKREDVERGQVVVKPGSITPHTNFEGTAYILSKDEGGRHNPFYTNYRPQF
                     YFRTTDVTGVITLPEGTEMVMPGDTTDMSVELIQPIAMEEGLGFAIREGGRTVGAGTV
                     TKINA"
     gene            complement(5557..7671)
                     /gene="fusA"
                     /locus_tag="VN041_06425"
     CDS             complement(5557..7671)
                     /gene="fusA"
                     /locus_tag="VN041_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWU28717.1"
                     /translation="MAQEVLTDLSKVRNIGIMAHIDAGKTTTTERILFYTGVNHKLGE
                     THDGASTTDWMEQEKERGITITSAAVTCFWNKNQINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFDGKEGVEPQSETVWRQADKYNVPRICFVNKMDKLGADFYYTVDTIVNRLGAK
                     PLVLQLPIGAENDFVGVIDLIEMRALVWPGDAKGDVTMGASYEIQEIPADLQAKAEEY
                     RQQLLETVAETDDALLEKFFGGEELTVAEIKGAIRKLVVANEIYPVLCGSAFKNRGVQ
                     PMLDAVVDFLPNPLDVGAIEAHDPKDEEKVIERHPDANDPFSALAFKVAVHPFFGRLT
                     YVRVYSGHLDSGSAVVNSTKGKKERIGKIFQMHANKENPVDSLTAGNIYAVIGLKDTT
                     TGDTLADPAAPVVLESMTFPEPVIEVAIEPKTKADQEKLGTAIQKLAEEDPTFRTELN
                     PETGQTTIKGMGELHLDILVDRMKREFKVEANVGKPQVAYRETIKKAVEKHDYTHKKQ
                     TGGSGQFAKIQFTIEPLEVTADKTYEFSNEVTGGRIPREYIEPTNQGFQDAMNTGVLA
                     GYPMVGVKARLIDGASHDVDSSEMAFKIAGSMGFKEAVRKANPVLLEPLMAVEVRTPE
                     EYMGDVIGDLNSRRGQIQSMEDAQGVKVVRASVPLSEMFGYIGDLRSKTSGRAVYSME
                     FDSYAEVPRAVADEIIQKTKGE"
     gene            complement(7752..8222)
                     /gene="rpsG"
                     /locus_tag="VN041_06430"
     CDS             complement(7752..8222)
                     /gene="rpsG"
                     /locus_tag="VN041_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWU28718.1"
                     /translation="MPRKGPAPKRPVVNDPVYGAPIVTSLVNKILVDGKKSLAESIVY
                     GALRGVEAKNGQDAVATLKKALDNVRPTLEVRSRRVGGSTYQVPVEVKPHRANTLALR
                     WLVSYAKGRREKTMTERLQNEILDASNGLGAAVKRREDTHKMAESNRAFAHYRW"
     gene            complement(8228..8596)
                     /gene="rpsL"
                     /locus_tag="VN041_06435"
     CDS             complement(8228..8596)
                     /gene="rpsL"
                     /locus_tag="VN041_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWU28719.1"
                     /translation="MPTIQQLVRKGRTPKVSKTKAPALKSNPQQAGVCTRVYTTTPKK
                     PNSAMRKVARVKLRNGTEVTAYIPGEGHNLQEHSLVLVRGGRVKDLPGVRYKIVRGAL
                     DTQAVKNRKQARSRYGAKKG"
     gene            complement(8825..>9585)
                     /locus_tag="VN041_06440"
     CDS             complement(8825..>9585)
                     /locus_tag="VN041_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28720.1"
                     /translation="DAPAALAAARWLTVPGALAFGIGVWPAIAEDWFVIWGMWTLMLG
                     WLALMVLTARRSSTTLPPVWVLFGLAFITAVVAWSPRELRVETFSLPLGLFLLAAGAL
                     GLRPAGGARGADAVGADRKRDVPGLDAWPHGWRGSWPLLAPGLIVMMSASVVSTFTDP
                     LTWRAILVMVLALAAILAGAARRLAAPFVIGLIVLPVENVFVFAVQLGRGIESMPWWI
                     TLATIGAVLLIIAVAGERREGADRSVVARMRDLR"
BASE COUNT         1469 a         3074 c         3272 g         1770 t
ORIGIN      
        1 cgcttctcgc cgtcgaagta gtgcagcagt gcgatgcgtg cggtgcggtt ggggtcgtac
       61 tcgatgtgag cgaccttggc gttgacgccg tccttgtcat tgcgacggaa gtcgatgacg
      121 cggtactggc gcttgtggcc accaccgatg tgacgggtcg tgatgcggcc ctggttgttg
      181 cgaccaccgg tcttcgagag cggacgcagc agcgacttct ccggcgtcga tcgggtgatc
      241 tcggcgaagt cggccaccga cgaaccgcgg cgacccgggg tcgtgggctt gtacttgcga
      301 atagccatta tctgtcctta tcccccggtc agccgactgc cgtgaagatg tcgatggaac
      361 ccgacttcag cgcgacgatg gcgcgcttgg tgtccttgcg cttaccggtg ccgaaacggg
      421 tgcggcgggc cttgccgacg cggttgatcg tgttgaccga agccaccttg acgccgaaga
      481 tcttctcgat ggcgagcttg atctcggtct tcgaggcgcg ggggtccacc aggaaggtgt
      541 acttgccctc gtcgatcaga ccgtagctct tctcggagac gaccgggccc aggatgatgt
      601 cgcgcgggtc cttgttcagg gccgttgcga ggatgttctg ctcagtcatg ccgagacctc
      661 ctcggttgcg ccggtcttcg aggcgatgaa ggcctcgagg gcggccttgg tgaagacgat
      721 gtcgtcggag acgaccacgt cgtaggcgtt cagctggtcg aagctgagca cgtgcacgtt
      781 cggaaggttc cggacgctct tcacggtcag ctcgtcgctg cgctcgatga cgacgagcac
      841 gttcttcgga ccggcgacgg cggcgagcag gcccgcagcg gtcttggtcg aaggcgtggc
      901 gtcggcgacg aaggcctcga cagcgtggag gcgctcaccg cggaagcggt cgctgagcgc
      961 gcccagcagg gcggcggcga tcatcttctt gggggtgcgc tgcgagtagt cgcgcggctt
     1021 cgggccgtgg acgatgccac caccggtcat gtgcggcgcg cggatggagc cctggcgggc
     1081 gttacccgtg cccttctgct tgaagggctt gcggcctgca ccggagacct caccgcgacg
     1141 cttggtcgag tgggtgccct ggcgtgcggc agcgagctgc gcgacgacga cctggtggat
     1201 cagcgggacg ttggtcttga cgtcgaagat ggcggcgggc agctcgatgg agccggcctt
     1261 cttgccgtcg actgcgatga cgtcgagcgc gagagtcgag tcagccatga tcaggcaccc
     1321 ttcactgcgt tgcggacgaa gacgatgcga ccacgtgcgc cggggacggc gcccttgacg
     1381 agcagcagac ccttctcgac gtcgacggcg tgcaccgtga ggttgaggac ggtcacgcgc
     1441 tcgccaccca tacggccggc catgcgcatg cccttgaaca cgcggctcgg ggtcgacgag
     1501 gcgccgatcg agccgggctt gcggtggttg cggtgcgcac cgtgcgatgc cgagacgccc
     1561 ttgaagttgt ggcgcttcat gacacccgcg gtgcccttgc ccttgctggt gcccacgacg
     1621 tcgaccagct ggccggcctc gaaggtgccg tccacggtca gctcctggcc gagcgcgtag
     1681 tcagcggcat ccgcggtgcg gatctcggtg acgtggcgac gcggcgtgac gccggcggcc
     1741 tcgaagtgag cggtgagcgg cttgttgacc ttgcgggggt cgatctgacc gtaggcgatc
     1801 tgaacggcgt tgtagccgtc cttctcgggg gtgcggatct gggtgaccac gttcggggcc
     1861 agctcgatga cggtgacggg aacgagcttg ccgttctcgt tccacacctg ggtcatgccg
     1921 agcttggtgc cgagcatgcc cttggagatc tttgcgttga tgtcagccat gtcgaacctc
     1981 agagcttgat ctcgatgttg acatcggccg gaaggtcgag acgcatcagc gagtcgacag
     2041 ccttgggcgt cgggtcgacg atgtcgatca gacgcttgtg ggtgcgcatc tcgaagtgct
     2101 cgcggctgtc cttgtacttg tggggggaac ggatcacggc gatcacgttc ttctccgtgg
     2161 gaagcggcac cgggccgacg acagtcgcgc ccgcacgggt cacggtgtcg acgatcttgc
     2221 gggccgacgt gtcgatgacc tcgtggtcat acgacttcag gcgaatgcgg atcttctgtc
     2281 ccgccattgt ctgctctctc tctataagcg tcttaccgac cggggtcggg tgaccctggg
     2341 gcattggacg cacctcggcg ctgttcacgc ctcggcactc gatcggctcc gaagaacctc
     2401 tctgcgcacc actgttctgc tgtcattccg cgcgccggaa cgcgcggcat ccgacttcct
     2461 gccatgcagg cacaccagga ccccgtgagg aggtgtcgga ttgtgtgttc tgctgcccgc
     2521 ggcctagaac cctgcgcatg ctcgtgatga gcgcgccgcc tatgcacttt cgtggcagtg
     2581 atcccgcgca tcgcgtagag cgcaggaatg tggaacctgt ctattctgcc atgaccgatt
     2641 cgcctactgc aaacccgggc gtgtcgcgct ccgattccgc gtgtttccgc gcgttcgcga
     2701 ggtgcggctc aagacgctgg gcggagcatc cccatgctcc gcccagcgct atgggaggcc
     2761 cggtgggagg ggacgcaccg ggcgagaggt gccgacgaga agggatgccg gtcgcctcgc
     2821 tcaggtgcgc gaccggacgg tcgcgcagac atggcgatgc atccgtggtc gacccacgtg
     2881 cgcggcacgg cgttccccat gatgaatccc cagtcattcg gatgctgtgc ccccacggca
     2941 tccttcgccg cacagatccc caggcggcgc tcacagtcta tggcgtgcag acgcggaatg
     3001 cgataacacc cacgcgatat atcagtggag agtaaggtac gtcccgaacc cctcggagca
     3061 cgcacgcaga atcggaacgg atgcgcagat ggacgactcg ctcgggacgc acaccaggga
     3121 cgctgcggcg atcccccgac gcacgctgat acgagccggc ttctggtccg tccccgtgat
     3181 agccgccgcc accgcggcac cgctggctgc ggcatccacg gtggggtcgt tggagatctt
     3241 cgcgacccgc tcgagcaccg ccatgctgcc gttgcagaca ctcgacggca cgtcggtgca
     3301 gccggaggcg accacgaccc tcccgatcgg cgtgacgatc tggaaccgcg ccggaagcac
     3361 gctggccgat tcgggcgcga cgctgacgct cgcctgcgac ctcccggact acacgctcgc
     3421 ctccggtatc gcgcgaggag tggcggtgta ctccttcgcc ggcgtcgtca tcccgtcgtc
     3481 cgcccggtcg atcgtctacg ccgaggacgg cgccggaacg ccggccggct tcccgcgcac
     3541 atccttcacg accgcactgc ccctggacat ctccgcgggc accttcagct atgacttcga
     3601 gctggagtgc cgcctgacga acgagacgga ctcgccgacg atcgactccg gtctgaactt
     3661 cccgttccgg atgacgctcc gcctctcaga cgggacggtg gcggccgaga actcacagcc
     3721 gctctatttc ggaccgggag cgaacgtgct ctgacccggt cccgacagcg gaacggcggt
     3781 ccctccgcag tggagggacc gccgttgcgc ggaatgcgcc gtgagactac tcgttgccga
     3841 cggctccgtc gacgttgtcg cggacgccgt cgatctgctc ggcggcaccc ggggcgacct
     3901 tcttggcgag ttcagccgct ccgtcgagga ccttgtcgct gatgccctcg gcctgctcgc
     3961 tgtgcagcag ctcctcgacc ttctccttgt tctgctcgaa cagttccttg ccctggttga
     4021 cgagatcctc gatgcccatg ggttcctccc cctgtcgctt cacgcagttc tctgcgccac
     4081 gtacagttct acgcgatgca cgcggctccg acaagagccg agatcgtccc ggcgcgtcgg
     4141 cggcggaccc gtggggaaga cgaaaggggc cgggcccgaa ggcccgaccc ctgaggtcgc
     4201 ttgagaagcg gaattacgcg ttgatcttcg tcaccgtgcc ggcgccgacg gtgcggccac
     4261 cctcacggat cgcgaagccg aggccctctt ccatggcgat cggctggatc agctcgaccg
     4321 acatgtcggt ggtgtcgccg ggcatgacca tctcggtgcc ctcgggcagc gtgatgacgc
     4381 cggtgacgtc cgtggtgcgg aagtagaact gcggacggta gttcgtgtag aaggggttgt
     4441 gacggccacc ctcgtccttc gagaggatgt aggcggtgcc ctcgaagttg gtgtgcgggg
     4501 tgatcgagcc cggcttcacg acgacctgac cgcgctcgac gtcctcgcgc ttggtgccgc
     4561 ggagcagcag accgcagttc tcgccggccc atgcctcgtc gagctgcttg tggaacatct
     4621 cgataccggt gaccgtggtc ttctgcgtcg ggcggatgcc gacgatctcg acctcggagt
     4681 tgatggccag ggtgccacgc tcggcgcggc ccgtgacgac ggtgccacga ccggtgatcg
     4741 tgaagacgtc ctcgatcggc atcaggaacg gcttgtcacg gtcgcgctcc gggtccggca
     4801 cgctgtcgtc gacggcctgc atgagctcga ggatcgcctt gacccacttc tcgtcgccct
     4861 cgagcgcctt cagcgccgag acctgcacga cgggagcgtt gtcaccgtcg aagccctgcg
     4921 acgagagcag ctcgcggacc tcgagctcga cgagctccag gatctcctcg tcgtcgacca
     4981 tgtcgctctt gttcagggcg acgagcaggt acggcacgcc gacctgcttg gcgagcagca
     5041 cgtgctcgcg ggtctgagcc atcgggccgt cggtggcggc gaccacgagg atcgcgccgt
     5101 ccatctgggc agcaccggtg atcatgttct tgacgtagtc ggcgtggccg ggggcgtcaa
     5161 cgtgcgcgta gtggcgcttc ggggtctcgt actcgatgtg cgagatgttg atcgtgatac
     5221 cgcgctgacg ctcctcggga gccgagtcga tcgaggcgaa gtcacgctgg acgttggtct
     5281 ccgacgggta ggtgtcggcg agcaccttcg agatcgcggc agacagcgtg gtcttgccgt
     5341 ggtcgacgtg accgatcgtt ccgatgttga cgtgcggctt ggtgcgctcg aacttggcct
     5401 tggccactgg gtcctcctca ggacgttcgt gtagagacgg ccgggcactg gattgtgacc
     5461 ggctccctac gggttaggtt tctcagttta gtagagaggg aatgtgaagt tgtagggagc
     5521 ccgggatgcc gttcgcacgg catcccggac aagggcttac tcgcccttgg tcttctggat
     5581 gatctcgtca gcgacggcgc gaggaacctc ggcgtagctg tcgaactcca tcgagtacac
     5641 ggcacggccc gaggtcttcg agcgcaggtc gccgatgtag ccgaacatct cggacagcgg
     5701 gaccgacgca cgcacgacct tgacgccctg ggcgtcctcc atcgactgga tctggccacg
     5761 acgcgagttc aggtcgccga tgacgtcgcc catgtactcc tcgggagtac ggacctcgac
     5821 ggccatcagc ggctcgagca gcacggggtt cgccttgcgg acggcctcct tgaagcccat
     5881 cgagccggcg atcttgaacg ccatctccga ggagtcgaca tcgtgcgatg cgccgtcgat
     5941 caggcgggcc ttcacgccga ccatggggta gccggcgagc acaccggtgt tcatggcgtc
     6001 ctggaagccc tggttggtcg gctcgatgta ctcgcgcggg atgcgaccac cggtgacctc
     6061 gttggagaac tcgtaggtct tgtcggccgt gacctcgagg ggctcgatcg tgaactggat
     6121 cttcgcgaac tgacccgaac caccggtctg cttcttgtgc gtgtagtcgt gcttctcgac
     6181 ggccttcttg atcgtctcgc ggtacgccac ctgcggcttg ccgacgttgg cctcgacctt
     6241 gaactcgcgc ttcatgcggt ccacgaggat gtcgaggtgc agctcgccca tgcccttgat
     6301 ggtcgtctga ccggtctcgg ggttcagctc ggtgcggaag gtcgggtcct cctcggccag
     6361 cttctggatc gcggtgccca gcttctcctg gtcggccttg gtcttcggct cgatggcgac
     6421 ctcgatcacg ggctccggga acgtcatcga ctcgaggacg accggggcgg ccgggtcggc
     6481 gagggtgtca ccggtggtgg tgtccttcag gccgatgacg gcgtagatgt tgccggcggt
     6541 cagcgagtcg accgggttct ccttgttggc gtgcatctgg aagatctttc cgatgcgctc
     6601 cttcttgccc ttggtcgagt tgacgaccgc ggagccggag tccaggtggc ccgagtacac
     6661 gcgcacgtag gtcagacgac cgaagaacgg gtgcacggcg accttgaagg cgagcgcgga
     6721 gaacgggtcg ttggcgtcgg ggtgacgctc gatgaccttc tcctcgtcct tcgggtcgtg
     6781 cgcctcgatg gcgcccacgt cgagcgggtt cggcaggaag tcgacgacgg cgtccagcat
     6841 cggctgcaca ccgcggttct tgaacgccga accgcacagc acggggtaga tctcgttcgc
     6901 gacgaccagc ttgcggatgg cgcccttgat ctcggcgacc gtgagctcct cgccgccgaa
     6961 gaacttctcg agcagcgcgt cgtcggtctc ggcgacggtc tcgagcagct gctgacggta
     7021 ctcctcggcc ttggcctgga ggtcggccgg gatctcctgg atctcgtacg acgcgcccat
     7081 ggtcacgtca cccttggcat cgccgggcca caccagtgcg cgcatctcga tcaggtcgat
     7141 gacaccgacg aagtcgttct cggcaccgat cgggagctgc agcacgagcg gcttggcacc
     7201 caggcggttc acgatggtgt cgacggtgta gtagaagtcg gcgcccagct tgtccatctt
     7261 gttgacgaag cagatgcggg gcacgttgta cttgtcggcc tgacgccaca ccgtctcgga
     7321 ctggggctcg acgccctcct tgccgtcgaa gacggcgaca gcaccgtcga gcacgcggag
     7381 cgagcgctcc acctcgaccg tgaagtccac gtggccgggg gtgtcgatga tgttgatctg
     7441 gttcttgttc cagaagcagg tcacggcggc agacgtgatc gtgatgccgc gctccttctc
     7501 ctgctccatc cagtcggtcg tcgaggcgcc gtcgtgggtc tcgccgagct tgtggttgac
     7561 acccgtgtag aacaggatgc gctcggtggt ggtggtcttg ccggcatcga tgtgcgccat
     7621 gatgccgatg ttgcggacct tgctcaggtc ggtgagcact tcttgtgcca cgggggttcc
     7681 tccgggatgg ttcaggaacg aacagttggt gggagcggat gccgcaggtc ctgcggcatc
     7741 cgctcagccc gttaccagcg gtagtgggcg aaggcgcggt tcgactcggc catcttgtgg
     7801 gtgtcctcgc ggcgcttgac cgcggcaccc agaccgttcg acgcgtccag gatctcgttc
     7861 tgcaggcgct cggtcatcgt cttctcacga cgacccttcg cgtagctgac cagccagcgc
     7921 agcgcgaggg tgttcgcacg gtgcggcttg acctcgacgg gcacctggta ggtcgagccg
     7981 ccgacgcgac ggctgcggac ctcgagggtg gggcgcacgt tgtcgagcgc cttcttgagg
     8041 gtggcgacgg cgtcctggcc gttcttcgcc tcgacgccgc ggagggcgcc gtagacgatc
     8101 gactcggcga gcgacttctt gccgtcgacg aggatcttgt tcaccagcga ggtgacgatc
     8161 ggagcaccgt agaccgggtc gttgacgacc gggcgcttgg gagcgggtcc cttacgaggc
     8221 atctaactca acccttcttc gcgccgtagc gggaacgagc ctgcttacgg ttcttgacag
     8281 cctgggtgtc cagggcgcca cggacgatct tgtagcggac accggggagg tccttcacac
     8341 ggccaccgcg cacgagcacc agcgagtgct cctgcaggtt gtgaccctca ccggggatgt
     8401 aggcggtcac ctcggtgccg ttgcggagct tcacacgagc gaccttgcgc atcgccgagt
     8461 tcggcttctt cggggtggtg gtgtacacac gggtgcacac gccggcctgc tgcgggttcg
     8521 acttcagggc gggcgccttg gtcttcgaga ccttgggcgt acgtcccttg cgaaccaact
     8581 gctgaatcgt tggcacgttc tctcctaata gtgctgcacg gtgacagcat gatgggtttc
     8641 acatcatgac ccaccggcag gacggaaccg tcctgctttt ggtgtgatgg gtatgccgtg
     8701 ggggcgaacc ggcggtgccg gcgcccagcg tgcgcgcaca gccgtgcgca cacccgatca
     8761 agtgtaatgc ggcgtaaccc cggcggtcaa atgacagcgg acgccacgcc ggtctgccga
     8821 gatctcatcg cagatcccgc atccgcgcga ccacactgcg gtcggcgccc tcgcgccgct
     8881 cccccgcgac ggcgatgatc agcagcaccg cgccgatggt cgccagcgtg atccaccacg
     8941 gcatggactc gatcccccgc ccgagctgca ccgcgaacac gaacacgttc tcgaccggca
     9001 gcacgatcag gccgatcacg aacggcgcgg ccagtcgacg cgcggccccg gccaggatcg
     9061 cggcgagcgc gagcaccatc acgaggatcg cccgccaggt gagcggatcg gtgaaggtgg
     9121 acacgacgga tgccgacatc atcacgatca ggcccggcgc gagcagcggc caggagcccc
     9181 gccagccgtg cggccaggcg tccaggccgg gaacgtcgcg ctttcggtcc gctccgacgg
     9241 catccgctcc cctcgctccg cccgcggggc gcaggccgag ggcgccggcg gcgagcagga
     9301 acaggccgag gggcagcgag aacgtctcca cgcgcagctc gcggggactc caggcgacga
     9361 cggctgtgat gaaggcgagg ccgaacagca cccacaccgg cgggagtgtc gtcgaggagc
     9421 ggcgtgcggt cagcaccatc agtgccagcc agccgagcat gagcgtccac atcccccaga
     9481 tcacgaacca gtcctccgcg atcgcaggcc acacgccgat gccgaaggcg agcgctccgg
     9541 gcacggtgag ccagcgagcg gccgccagcg ccgcgggggc gtcgg
//
LOCUS       DATMSX010000205         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_31207,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000205 DATMSX010000000
VERSION     DATMSX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31207"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..931
                     /locus_tag="VN041_06445"
     CDS             194..931
                     /locus_tag="VN041_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HWU28721.1"
                     /translation="MAKESIFGRISTLVRANINALLDQAEDPQKMIDQLVRDYTNNIA
                     DAESAIAETIGNLRLLERDHEEDVQAATDWGNKALAASRKADELRGIGHTADADKFDN
                     LAKIALQRQISSEREAKAAEPQIASQTEIVDKLKDGLNGMKNKLGELKTKRSELLARA
                     KVAEAQTKVQDAIGSINVLDPTSELGRFEDKIRRQEALAQGKAEIAASSLDAQFESLE
                     DLGELTEVEARLAELKSGKPQAAIEAE"
     gene            1009..1836
                     /locus_tag="VN041_06450"
     CDS             1009..1836
                     /locus_tag="VN041_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWU28722.1"
                     /translation="MTRFLIVPQWQGSPASRAMLLVDGATAIAGDLPRKDTTVLDVPL
                     EAGESLGTGVRRLSSLLRVRELIVEHMTDADDQIVLVGGDCSVSVFALSAIDTSDLAV
                     LWCDAHPDLNDPSTSPSGAFSGMSLGAVLGDGEPQLALNPGIPAERVVLLGARSVDDG
                     EKSRLDALRTLSPEDAQDPDAVAEALRATGASRVWVHIDVDVIDPSEFEGVSDAEPFG
                     VSTAALAAAVKRVRQDLPLAGATIAGFAPRSPADAVQDMGAILRLIGSVAWRGARHE"
     gene            1846..2286
                     /locus_tag="VN041_06455"
     CDS             1846..2286
                     /locus_tag="VN041_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HWU28723.1"
                     /translation="MSADWRADAERALDRGRRSDLRIPSFLLRSPISRIGYWWGTAVG
                     WVWGSLWSTGPVERRAGLWVFRGLPAWTYGRGGVCVGGCYLTGDVEPSDAVLRHEGVH
                     KQQWLRYGFLMPVLYLFAGRNALRNRFEIEAGLEDGGYVPRSRD"
     gene            complement(2290..>2541)
                     /locus_tag="VN041_06460"
     CDS             complement(2290..>2541)
                     /locus_tag="VN041_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWU28724.1"
                     /translation="MTVTWLPRTRRGIRRAAGETLTRAATAWADEMLCDDSEGAMRTE
                     ATLLGGYLSTADIVEHLTGSCGVEPTAIHAPARYGLPVF"
BASE COUNT          399 a          892 c          871 g          379 t
ORIGIN      
        1 ggcggcggag gcggcggagg cttctccgtc ggcggcttcg gcggaggagg cggcggcttc
       61 ggcggcagca gcggaggcgg cggctcgttc tgaccgcatg cgcacctgat cgcgactcat
      121 cagatcgacc tctaacctga ccagacacgt acacggcaag acaaccatcc gagaagaaaa
      181 gggaacccac acaatggcca aggaatccat cttcggacgc atctccacgc tcgtccgtgc
      241 caacatcaac gctctcctcg accaggccga ggatccgcag aagatgatcg accagctcgt
      301 ccgcgactac acgaacaaca tcgccgacgc cgagtccgcg atcgcggaga ccatcggcaa
      361 cctgcgtctt ctcgagcgcg accacgagga ggacgtgcag gctgcgacgg actggggtaa
      421 caaggccctc gccgcctccc gcaaggccga cgagctgcgc ggcatcggcc acaccgccga
      481 cgccgacaag ttcgacaacc tcgcgaagat cgcgctgcag cgccagatca gctccgagcg
      541 cgaggcgaag gcggccgagc cgcagatcgc gtcgcagacc gagatcgtcg acaagctgaa
      601 ggacggcttg aacggcatga agaacaagct cggcgagctg aagaccaagc gcagcgagtt
      661 gctcgcccgt gccaaggtcg ccgaggcgca gaccaaggtt caggacgcca tcggctcgat
      721 caacgtgctg gacccgacca gcgagctggg ccgcttcgag gacaagatcc gccgccagga
      781 ggctctcgcc cagggcaagg ccgagatcgc tgcctcgtcg ctggacgcgc agttcgagag
      841 cctcgaggac ctcggtgagc tcaccgaggt cgaggcgcgc ctcgctgagc tcaagtcggg
      901 caagccgcag gccgccatcg aagccgagtg atcggacaca cagattgacc gagcgggcgg
      961 acgccgcatc ccggcatccg cccgctcctt ctcctcccag gagagaccat gacccgtttc
     1021 ctcatcgtcc cgcagtggca gggctcgccc gcctcgcggg ccatgcttct cgtcgacggc
     1081 gccaccgcga tcgccggcga cctgccccgc aaggacacca ccgtcctgga tgtgccgctc
     1141 gaggcgggtg aatcgctcgg caccggcgtg cgccggctca gctcgctctt gcgcgtgcgg
     1201 gagctgatcg tggagcacat gacggatgcc gatgatcaga tcgtgctcgt cggcggcgac
     1261 tgcagcgtga gcgtgttcgc gctctccgcg atcgacacct cggatctcgc cgtgctctgg
     1321 tgcgacgcgc atcccgatct gaacgatccc tccacctccc cgtccggcgc gttctccggc
     1381 atgtccctcg gagccgtgct cggcgacggc gagccgcagc ttgccctgaa cccgggcatc
     1441 cccgccgagc gggtcgtcct cctcggcgcc cgcagcgtcg atgacggcga gaagtcgcgg
     1501 ctggacgcgc tgcgcacgct ctcgccggag gacgcgcagg atccggatgc cgtggccgag
     1561 gcgctgcgcg cgaccggggc atcccgggtc tgggtgcaca tcgacgtcga tgtcatcgat
     1621 ccctcggagt tcgagggtgt ctctgatgcg gagccgttcg gcgtcagcac cgccgccctc
     1681 gccgccgccg tcaagcgcgt ccgtcaggat ctcccgctcg ccggcgcgac gatcgccggg
     1741 ttcgcgcccc gcagccctgc ggatgccgtg caggacatgg gcgcgatcct gcgcctgatc
     1801 ggatccgtgg cgtggcgagg cgcgcggcat gagtaggacc gcaccatgag cgccgactgg
     1861 cgcgccgacg ccgagcgcgc actcgatcgg ggacgacgct ccgacctgcg catcccctcc
     1921 tttctgctgc gctcccccat cagccggatc ggctactggt ggggcactgc cgtcggctgg
     1981 gtctggggct cgctctggag caccgggccg gtcgagcgcc gcgcgggtct ctgggtgttc
     2041 cgcggcctgc cggcctggac ctacggtcgc ggcggggtct gcgtgggcgg ctgctatctg
     2101 accggtgatg tcgagccgtc ggatgccgtg ctgcgtcacg agggcgtgca caagcagcag
     2161 tggctgcgct acggcttcct catgcccgtg ctgtacctgt tcgccggccg gaatgcgctg
     2221 cgcaaccgct tcgagatcga ggcggggctg gaagacggcg gctatgtgcc gcgttcccgc
     2281 gactgagcgt cagaagaccg gcagtccgta tcgtgcggga gcgtggatgg ccgtcggctc
     2341 gacgccgcac gatccggtga ggtgctcgac gatgtctgcc gtgctgaggt agccgcccag
     2401 cagcgtggcc tcggtgcgca tcgcaccctc ggagtcgtcg cacagcatct cgtccgccca
     2461 tgcggtcgcg gcgcgtgtga gcgtctctcc ggccgcccgg cggatgcccc ggcgggtgcg
     2521 aggcagccag gtgacggtca t
//
LOCUS       DATMSX010000206         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_144785,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000206 DATMSX010000000
VERSION     DATMSX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144785"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(540..>1884)
                     /locus_tag="VN041_06465"
     CDS             complement(540..>1884)
                     /locus_tag="VN041_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWU28725.1"
                     /translation="PPATCELGGGMATAYHRRPVLSRRDVAALAHGKIGNGSAWQGYY
                     MYVGGRNPGPGLQETQATGYPNDMTEWSYDFHAPIGQSGDLHGSAALLRAQHAFLSAF
                     GDRLGGMTSTLPELQADGLDDTATLRWALRSDGAEGFCVITHHQPHIPVQPVRGVQLR
                     VELDDEALLLPRTPIDIPAGTIARWPVGLRIGDARLRWATASALTVLPGGVLVLAADP
                     GIPVEVAVSDEEPRVVTPGLHEIGGLTLLVLADAADAWVLGDELWLCDGQLLWDGSYV
                     QALDATRLRRFDTATRTWVETPVDRPSDAVPVGIRTVRPAGEVPADHGFHGPRHSAPA
                     AETFDALAAEFALELPRWDGDALLDIDWTGDAAQLRVDGATVDDRFWDGTRWSVCLRD
                     VGADAGSTLSLHVLPLSRESTVWLPEGAQQKRGAAAEGVVAAVSLRVRKSARVSG"
BASE COUNT          278 a          665 c          662 g          279 t
ORIGIN      
        1 gagcgtggcc gcggcggggg cggccgtgag tgcggcgccg atgacggcgg cgaccgtggc
       61 cgcggcgagc gtgcagtgtg ttcgtcgtgc gaagagcggt cgtgcagaac gtgtggaatg
      121 gtccacggtt cctccttggg cgtctctgac aaaggggttc cggcgtgcgc cgcagcatcg
      181 tagagcacgt cgaaggaacc ggggcgcgta cggactcggc cacggatgga gcagctggaa
      241 aacgcattcc cagctgcttc cgatgatcct tgcatgagct ccacagggtg agcaagcgtt
      301 ttctgacacg tttcaaagaa ttcgatgaaa ctgttgtgca acgttgaaga gttggttacg
      361 gtcggaacga ccctcaccgg ccggtacccc gtgccggttc cagagctccc gctgtcggcg
      421 cgattacgga ccgtgtcggc agcgggtccc acgcccggac cgaccccgga ctccccactt
      481 ccgtcgccgg ccctccggcg tccggccgct gagcccgtcg aagcgtcgac tgctcctgct
      541 cagccgctga cccgggcgct cttcctcacc cgcagcgaca cggcggcgac gacaccctcc
      601 gcggccgcgc cgcgcttctg ctgggcccct tccggcagcc acaccgtgct ctcccgcgac
      661 agtggaagca catgcagact cagggtgctg ccggcatccg ctccgacgtc gcgcaggcag
      721 accgaccagc gggtaccgtc ccagaagcga tcgtcgaccg tcgcgccgtc gacccgcagc
      781 tgggccgcat cgcccgtcca gtcgatgtcg agcagcgcgt cgccgtccca ccggggcagc
      841 tcgagcgcga actccgcggc aagtgcgtcg aatgtctccg cagcaggcgc actgtgcctc
      901 ggcccgtgga agccgtggtc ggcgggaacc tctccggccg gcctgaccgt gcggatgccc
      961 actggcacgg catccgaggg gcggtcgacc ggagtttcca cccacgtgcg cgtggcggtg
     1021 tcgaagcggc gcaggcgggt ggcgtcgagc gcctggacgt atgagccgtc ccacagcagc
     1081 tgcccgtcgc agagccacag ctcgtcgccg agcacccagg catccgcggc atcggcgagg
     1141 actagcagcg tgagtccgcc gatctcgtga agccctggtg tcacgacgcg cggctcctca
     1201 tcggatacgg cgacctccac cggtatgccc gggtcggcgg ccagcaccag gacgcccccc
     1261 ggcagaaccg tcagagcgga tgccgtcgcc caccgcagcc gtgcgtcccc gatccgcagt
     1321 cccaccggcc agcgcgcgat ggtgcccgcc ggtatgtcga tcggggtgcg ggggagcagc
     1381 agtgcctcgt cgtcgagctc gacccgcagc tgcacgccgc gcacgggctg caccgggatg
     1441 tgcggctgat gatgcgtgat cacgcagaac ccctcggcgc cgtccgagcg cagtgcccag
     1501 cgcagcgtgg cggtgtcgtc gagaccgtcg gcctgcagct cggggagggt cgaggtcatg
     1561 ccgccgagac ggtctccgaa cgccgagagg aacgcatgct gggcgcgcag cagtgcggcg
     1621 gatccgtgca gatcgccgga ctgtccgatc ggggcgtgga aatcgtagct ccactcggtc
     1681 atgtcgttcg ggtagcccgt cgcctgcgtc tcctgcaggc cgggacccgg gttccgcccg
     1741 ccgacgtaca tgtagtaccc ctgccaggcc gacccgttgc cgatcttccc gtgcgcgaga
     1801 gcggccacgt cgcggcggct gagcacgggg cgccggtgat aggcggtggc catgccgccg
     1861 ccgagctcgc acgtcgcggg cggg
//
LOCUS       DATMSX010000207         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_154594,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000207 DATMSX010000000
VERSION     DATMSX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154594"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VN041_06470"
     CDS             <1..255
                     /locus_tag="VN041_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWU28726.1"
                     /translation="GDGDAASLPAGAKVMPNEARLERYIGSAPPAGTGVHRYFFVIDA
                     LDVESLDLDGDETPGTLGFHRHFHTLARGILIGTADPAER"
     gene            313..1158
                     /locus_tag="VN041_06475"
     CDS             313..1158
                     /locus_tag="VN041_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWU28727.1"
                     /translation="MRLATWNINSIRTRVGRAVDFAVREDIDVLAFQEIKCKPEQFPY
                     APFEEAGYHVEVHGLSQWNGVAIASREPITEVSTSFAGMPGFAKGHEGPDAPLEARAL
                     GVTVGGVRVWSLYVPNGRSLDDPHMAYKLHWLDALRTATAAEIAANPSRPLALVGDFN
                     IIPFDHDNGDPDIVVGRSTHVSPEERAAFFALESAGLSDVVRPLIPEGFTYWDYQRLK
                     FPRNEGIRIDFVLGSDAFAGAVTAASIHREERKGEQPSDHVPVVVELDLGGPEEDDDL
                     PMIFA"
     gene            1155..1967
                     /locus_tag="VN041_06480"
     CDS             1155..1967
                     /locus_tag="VN041_06480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWU28728.1"
                     /translation="MSIRLIATDLDGTLLGDDGAVSPRTRAALDAARNAGITTIPVTA
                     RQPIGLRPIAAQAGFTDWALCGNGAYGVHLTTGEHLFAEEIPPAVQQELADALNSVLP
                     GLVYASVRDAGEGFVAQEGYATLAKLSDHKRDPETMGGVPLADVLALPSLKLVIRHPS
                     VSIPEIYAALQSLGLTGFEATLSGAPFVEVMAQGVTKATGVAQVCERLGIDRSEVLAF
                     GDALNDLEMLRWAGRGVAVGNAIDVVRDAADEVAPSNADDGVAQVIERMLAG"
     gene            2139..>2653
                     /locus_tag="VN041_06485"
     CDS             2139..>2653
                     /locus_tag="VN041_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28729.1"
                     /translation="MTTQSEYPQPPLSLTKGLGPALAHDDPARLIGSSLFAADRDFPQ
                     LTLNGPSIAHNIEAMMGYVRDRGVSIAPHGKTAMSPQLAQQQLDAGAWGITAATPFQL
                     RVYRAAGMPRLLLANQLVEPGAIAWIRGELAADPDFECLVYADSTAGLDLLADPAAAD
                     GDRPLAVLLEAG"
BASE COUNT          389 a          958 c          923 g          383 t
ORIGIN      
        1 ggcgacggcg acgccgcgtc cctccccgcc ggcgcgaagg tgatgccgaa cgaggcgcgc
       61 ctggagcgct acatcgggtc agcaccgccg gcgggcaccg gcgtgcaccg ctatttcttc
      121 gtgatcgacg cgctggacgt cgagagcctc gacctcgacg gcgacgagac gccgggcacg
      181 ctcggattcc accggcactt ccacacgctc gcccgcggca tcctgatcgg caccgctgac
      241 ccggccgagc gctgagggcg cgggtccacg ggcccggtga gtgacgtcgg agaccccgaa
      301 taggctggac gcatgcgtct ggccacctgg aacatcaact cgatccgcac gcgcgtgggg
      361 cgcgccgtcg acttcgcggt ccgcgaggac atcgacgtgc tcgcgttcca ggagatcaag
      421 tgcaagcccg agcagttccc ctatgcgccg ttcgaggaag ccggatacca cgtcgaggtg
      481 cacggcctta gccagtggaa cggcgtcgcg atcgcgagcc gtgagccgat caccgaggtg
      541 tccacctcgt tcgccggaat gccgggcttc gcgaagggcc acgagggtcc ggatgccccg
      601 ctcgaggcgc gtgcgctggg cgtgacggtg ggcggcgtgc gggtttggag cctctacgtg
      661 cccaacggcc gttcgctgga cgacccgcac atggcgtaca agctgcactg gctcgacgcg
      721 ctgcgcaccg cgaccgccgc ggagatcgcc gcgaacccga gccgcccgct ggcgctggtc
      781 ggcgacttca acatcatccc gttcgaccac gacaacggcg accccgacat cgtggtcggc
      841 cgctcgacgc acgtctcgcc cgaggagcgc gcggcgttct tcgccctgga gtcggctggc
      901 ctgtccgatg tggtgcgccc gctcatcccg gagggcttca cctactggga ctaccagcgg
      961 ctcaagttcc cccgcaacga gggcatccgc atcgacttcg tgctcggttc cgacgccttc
     1021 gcaggagccg tcaccgcggc atccatccac cgcgaggagc gcaagggcga gcagcccagc
     1081 gaccacgtgc cggtggtcgt ggagctcgac ctcggcgggc cggaggagga cgacgacctg
     1141 ccgatgatct tcgcgtgagc atccgcctga tcgctaccga tctcgacggc actctgctgg
     1201 gcgacgacgg cgcggtctcg cctcgcacgc gcgcggcgct ggatgcggcg cggaatgccg
     1261 ggatcacgac gatcccggtg acggcgcgtc agccgatcgg gctgcgaccg atcgccgcgc
     1321 aggcgggctt caccgactgg gcgctgtgcg gcaacggcgc atacggtgtg cacctgacca
     1381 ccggcgagca cctgttcgcc gaggagatcc cgccggccgt gcagcaggag ctcgccgacg
     1441 cgctgaacag cgtgctgccc ggcctcgtct acgcgagcgt gcgggatgcc ggtgagggct
     1501 tcgtcgcgca agagggctac gccaccctcg ccaagctgag cgaccacaag cgcgatccgg
     1561 agacgatggg cggtgtgccg ctggcagacg tgctggcgct tcccagtctg aagctcgtga
     1621 tccggcatcc gtcggtctcg atccccgaga tctacgccgc gctgcagtcg ctggggctga
     1681 ccggcttcga ggccacgctg tcgggtgcgc ccttcgtcga ggtgatggcc cagggcgtga
     1741 ccaaggcgac cggcgtcgct caggtgtgcg agcggcttgg catcgatcgc agcgaggtgc
     1801 tggccttcgg cgacgcgctg aacgacctcg agatgctgcg ctgggcgggc cgcggcgtcg
     1861 ccgtcgggaa cgccatcgac gtggtgcggg acgccgccga cgaggtggcg ccgtcgaacg
     1921 ccgacgacgg cgtcgcgcag gtgatcgagc ggatgctggc gggctgacgg cgtacctcgc
     1981 attccatatg cgcagtgcag cgttgatggc tgcgttgcag aatggatacc catgtgccac
     2041 cgtgaacggg tccccgagct ggcgccgatg gtcatcgccg cggccgccga caactcccgg
     2101 cgcatgggcg cccccgcttc cgcacggagg tcattcgcat gacgacgcag tccgaatacc
     2161 cgcagccccc gctgagcctg accaaggggc tcggccccgc actggcgcac gacgacccgg
     2221 cgcggctcat cggcagctcg ctgttcgccg ccgatcgcga cttcccgcag ctcacgctga
     2281 acgggccctc catcgcgcac aacatcgagg cgatgatggg ctacgtccgc gaccgcggag
     2341 tgtccatcgc cccgcacggc aagaccgcca tgtcgcctca gctcgcccag cagcagctcg
     2401 acgccggcgc atggggcatc accgccgcga cgcccttcca gttgcgcgtg taccgtgccg
     2461 ccggcatgcc ccggctgctg ctggccaacc agctcgtcga gccgggcgcg atcgcctgga
     2521 tccgcggcga gctcgcggcc gaccccgact tcgagtgcct ggtctacgcg gactccaccg
     2581 cgggcctcga cctgctcgcc gatccggccg ccgctgacgg ggaccgcccg ctcgccgtgc
     2641 tgctcgaggc ggg
//
LOCUS       DATMSX010000208         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_88364,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000208 DATMSX010000000
VERSION     DATMSX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88364"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VN041_06490"
     CDS             <1..702
                     /locus_tag="VN041_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWU28730.1"
                     /translation="DARRALTGLEAAAAVALSRGDDAEITADDVAQAVDRALLRYDRQ
                     GDEHYDVISAFIKSIRGSDPDAAVHYLARMIEAGEDPRFIARRLVISAAEDIGLADPQ
                     ALSIATAAADAVAFIGMPEGRIPLAEATIYLATTAKSNAAYLAIDAAIADVRGGGFGR
                     VPIHLRDAHYAGAKRLGHGKGYRYPHDSDAGILPQQYLPDELVGRHYYEPKNLGAERD
                     VSARLERIRRILGDR"
     gene            875..1504
                     /gene="rpsD"
                     /locus_tag="VN041_06495"
     CDS             875..1504
                     /gene="rpsD"
                     /locus_tag="VN041_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWU28731.1"
                     /translation="MVTKSQDRRKVRLSRALGIPLTPKAARYLEKRPYAPGEHGRTKR
                     KADSDYAVRLREKQRLREQYGIREKQLRIAFNEARRKDGLTGENLVELLEMRLDALVL
                     RAGFARTTAQARQLVVHRHILVDGQLVDRPSFRVKPGQLIHVKAKSEALEPFQVAAAG
                     GHAEVLPPVPGYLEVELDKLQARLVRRPKRVEVPVTCEVQLVVEYYAAR"
     gene            1568..1759
                     /locus_tag="VN041_06500"
     CDS             1568..1759
                     /locus_tag="VN041_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28732.1"
                     /translation="MKNLLWFLIGVIGGFVAAHFMNKDPRGQEIFADIDARISEFTGI
                     LGEAYRQQEARLADTADEA"
     gene            1830..>2380
                     /locus_tag="VN041_06505"
     CDS             1830..>2380
                     /locus_tag="VN041_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWU28733.1"
                     /translation="MRTAEIAQRYLDYFEKNDHLIVPSASLVSDDPSLLFTVAGMVPM
                     IPYLTGVVPAPHPRIADVQKCIRTNDIEEVGRTARHGTFFQMLGNWSFGDYFKEGAIR
                     YAWELLTSSESDGGLGFDEKDLWVTVYETDDEAEAIWRDIIGLKPERIQRLGRADNYW
                     NTGQPGPGGPDSEIFFDRGPAYGR"
BASE COUNT          400 a          841 c          776 g          363 t
ORIGIN      
        1 gacgcccgcc gggcactgac cgggctcgag gccgccgccg ccgtcgcgct gtcacgcgga
       61 gacgatgcgg agatcaccgc cgacgacgtc gcgcaggcgg tggatcgcgc gctgctgcgc
      121 tacgaccgcc agggcgatga gcactacgac gtgatcagcg cgttcatcaa gtcgatccgc
      181 gggtcggacc cggatgcggc cgtgcactac ctggcgagga tgatcgaggc gggggaggac
      241 ccgcgcttca tcgcccggag gctggtcatc tccgcggcgg aggacatcgg tcttgcggat
      301 ccgcaggcgc tgtcgatcgc caccgcggcg gcggatgccg tggccttcat cggcatgccg
      361 gaaggccgca tcccgctcgc cgaggcgacc atctacctcg ccacgacagc caagtcgaac
      421 gccgcgtacc tggcgatcga cgccgcgatc gccgatgtgc ggggcggcgg cttcgggcgc
      481 gtgcccatcc acctgcgcga cgcgcactat gcgggcgcga agcggctcgg tcatggcaag
      541 ggctaccgat acccgcacga cagcgatgcc ggcatcctgc cgcagcagta cctgcccgac
      601 gagctcgtcg gccggcacta ctacgagccg aagaacctcg gtgccgagcg cgacgtctcc
      661 gcccggctcg agcgcatccg ccgcatcctc ggcgaccgct gagacgtccg ggctcatctg
      721 atagcatgga ccggctcgaa acccagtttt cgggcatccc tccattcctg aagcacttcc
      781 cggcgcgcct cggcgtacgc ggcgggaagg gggagcagag gagatacgcc cgcgagtcgt
      841 gaaagacacg tccgcgtcat ggaaggagca cttcgtggtc acgaagtctc aggaccgccg
      901 caaggtccgc ctcagccgcg cgctgggcat cccgctcacc ccgaaggccg cccgctacct
      961 cgagaagcgt ccctacgccc cgggtgagca cggccgcacc aagcgcaagg ccgacagcga
     1021 ctacgccgtc cgtctgcgcg agaagcagcg tctgcgcgag cagtacggca tccgcgagaa
     1081 gcagctgcgc atcgcgttca acgaggcccg ccgcaaggac ggcctgaccg gtgagaacct
     1141 ggtcgagctg ctcgagatgc gtctggacgc cctcgtgctg cgtgccggct tcgcccgcac
     1201 caccgcgcag gcccgtcagc tggtcgtgca ccgtcacatc ctggtcgacg gtcagctcgt
     1261 cgaccgcccg tccttccgcg tgaagccggg tcagctcatc cacgtcaagg ccaagtccga
     1321 ggctctcgag cccttccagg tcgcagccgc cggcggtcac gccgaggtcc tgcccccggt
     1381 cccgggctac ctcgaggtcg agctcgacaa gctgcaggcc cgtctggtcc gtcgtccgaa
     1441 gcgcgtcgag gtccccgtga cctgtgaagt gcagctggtc gtcgagtact acgcggctcg
     1501 ctgatacagc atccgtgcga gaggcgtcgg ggggattccc cggcgcctct cgtcgtagga
     1561 tggcggaatg aagaatctgc tctggttcct gatcggtgtg atcggcggtt tcgtcgccgc
     1621 gcacttcatg aacaaggacc cccggggtca ggagatcttc gccgacatcg acgcgcgcat
     1681 ctcggagttc accggcatcc tcggtgaggc gtaccgccag caggaagcgc gtctggcaga
     1741 cactgcagac gaagcctgat tccgacgatt cccccatccc gtcgcgtcct cggacgcggc
     1801 tccgccacgc aaggaaaccc acgagcacta tgagaactgc ggagatcgcg cagcggtacc
     1861 tcgactactt cgagaagaac gaccacctca tcgtcccttc ggcttcgctg gtcagcgatg
     1921 acccgtcgct gctgttcacg gtcgccggta tggtgccgat gatcccgtat ctcaccggtg
     1981 tcgtcccggc cccgcatccg cgcatcgccg acgtgcagaa gtgcatccgc acgaacgaca
     2041 tcgaggaggt cgggcgcacc gcgcgtcacg gcaccttctt ccagatgctc ggcaactggt
     2101 cgttcggcga ctacttcaaa gaaggcgcca tccgctacgc ctgggagctg ctgacgagct
     2161 cggagtccga tggcgggctc ggcttcgatg agaaggacct ctgggtcacg gtctacgaga
     2221 ccgacgacga ggccgaggcc atctggcgcg acatcatcgg gctgaagccg gagcgcatcc
     2281 agcgcctcgg tcgtgcggac aactactgga acaccggtca gcccggcccc ggcggcccgg
     2341 actccgagat cttcttcgac cgcggacccg cctatggccg
//
LOCUS       DATMSX010000209         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_163839,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000209 DATMSX010000000
VERSION     DATMSX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163839"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VN041_06510"
     CDS             complement(<1..520)
                     /locus_tag="VN041_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28734.1"
                     /translation="MDGPVLSGGVIVVVAVLLWALYFLPSWRGRHQYYAAERNAVRLN
                     QALRVLAETSETPEEVRLELTARTVMQQQRLAKRVQAEKENAELERLRQELAATRRDP
                     LVRQARARRRLRLTASTGLVIATAMIGFGVWDYLSTGGWMLAASGAAVAVLGVMVLVR
                     MAQVARNAVRRAA"
     gene            complement(590..1213)
                     /locus_tag="VN041_06515"
     CDS             complement(590..1213)
                     /locus_tag="VN041_06515"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWU28735.1"
                     /translation="MAIDGFDPMQHGPVGLRLVRSRDARTLQRELLSNRSWLRPWEAT
                     IPGGVATFDMRVSIRRLLQQHRDGAGYPFVMEYDGEIAGQLNIWGVARGSLGSATIGY
                     WVSERFAGRGITPTAVAMATDAAFDQFGLHRMEICIRPENRASLRVVEKLGFRYEGLR
                     RRYIHIDGDWRDHHAFALVKEDVPEGVLTRWVQGRVPPGLGVAPSEI"
     gene            complement(1227..2108)
                     /gene="galU"
                     /locus_tag="VN041_06520"
     CDS             complement(1227..2108)
                     /gene="galU"
                     /locus_tag="VN041_06520"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWU28736.1"
                     /translation="MSKMKAVIPAAGLGTRFLPATKAMPKEMLPVVDKPAIQYVVEEA
                     VSAGIDDILVIIGRNKNAISDHFDSVPELEVRLQQKGDHGRLERVMKSSDLADIHFVR
                     QGEPKGLGHAVLRARTHVGDSSFAVLLGDDLIDERDPLLTEMIAAHERTGAAVIALMQ
                     VPAESIHLYGVAATDGDDLDGAVRVTGLVEKPSAEDAPSDLAVIGRYVLPPAVFEVLE
                     RTQPGKGGEIQLTDALQELATDPDGPGVVGVVFRGRRYDTGDRVDYIKAIVQLAVDRD
                     DLGADLRPWLKDFAGGL"
     gene            2177..2773
                     /locus_tag="VN041_06525"
     CDS             2177..2773
                     /locus_tag="VN041_06525"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWU28737.1"
                     /translation="MPGDEEQQKRALRAELRERRQLLSDSQRESASDGVAAQLHELIR
                     SHGARSVSCFLSTTTEPGTRRFVREAVATGIRVLLPITRADGLLDWAVANDSDEVSEG
                     LYGLPEPTGEVLGPIAVNDVDLMIIPAAAIDRTGTRLGWGRGYFDKTIGSMEKCPPVY
                     AVIYDSEVLDSLPREVHDQPVDGIVTPTQTIALSPARH"
     gene            2812..3084
                     /locus_tag="VN041_06530"
     CDS             2812..3084
                     /locus_tag="VN041_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWU28738.1"
                     /translation="MPTYAYACTACDHRFDAVQSFSEDSLTVCPECGGALRKQYGTVG
                     VTFNGSGFYRTDSRGNTGATTGSPASSKVDSSTGSNPAPAKTPASS"
     gene            3112..3492
                     /gene="mscL"
                     /locus_tag="VN041_06535"
     CDS             3112..3492
                     /gene="mscL"
                     /locus_tag="VN041_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWU28739.1"
                     /translation="MFQGFKEFIAKGNVIDLAVAVVIGAAFSAIIAALVDNLINPLIG
                     LIFNAQDLNHVKLGVIGIGAIIGAVINFLAVALIVYFVFVLPMNKYKEHQAAKNPAVE
                     EETLPSEQELLIEIRDALRKNAAG"
     gene            complement(3575..3754)
                     /locus_tag="VN041_06540"
     CDS             complement(3575..3754)
                     /locus_tag="VN041_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28740.1"
                     /translation="MSDEDRQHIERVPGARRARLTPVAGTTAEPHVREERHAGTEGDH
                     GPNDDRMLREVPPHY"
     gene            complement(3747..>4010)
                     /locus_tag="VN041_06545"
     CDS             complement(3747..>4010)
                     /locus_tag="VN041_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28741.1"
                     /translation="LLPLVAQHGGRAVVIESDPESRDESLRESLRLRPQVLRRLGWHY
                     VRVHAFDLYSDPATVARRIATVLGIEEDAVRADNDTQPFDVDE"
BASE COUNT          618 a         1449 c         1325 g          618 t
ORIGIN      
        1 ccgcagcgcg gcggacggcg ttccgcgcga cctgcgccat gcgcaccagc accatcacgc
       61 cgagcacggc gacggcggct ccggatgccg ccagcatcca gccgccggtg gagagatagt
      121 cccagacgcc gaagccgatc atggcggtgg cgatgaccag tccggtactg gccgtgaggc
      181 gcagccggcg acgggccctg gcctgacgga cgagcggatc ccgcctggtc gccgcgagct
      241 cctgacggag acgctccagc tcggcgttct ccttctcggc ctgcacgcgc tttgcgagtc
      301 gctgctgctg catcaccgtg cgcgcggtga gctcgaggcg gacctcctcg ggggtctcac
      361 tggtctcggc cagcacccgc agcgcctggt tcagccggac ggcgttgcgc tcggcggcgt
      421 agtactggtg ccgcccccgc cagctgggca ggaagtacag tgcccacagc aggacggcga
      481 cgacgacgat cacgccccca ctcagcaccg gcccatccat agagaccacg gtaagcgagc
      541 ggatccgaac cgccgcgaaa cagccttagc gtgtcgcggc gattgcgtct cagatctcag
      601 acggagcgac cccgaggccc ggcgggacgc gcccctgcac ccagcgtgtc agcacaccct
      661 ccggcacgtc ctccttgacc agggcgaacg cgtgatggtc gcgccagtcg ccgtcgatgt
      721 ggatgtaccg ccgacgcagc ccctcgtacc gaaagccgag cttctccacc acacgcaggc
      781 tcgcgcggtt ctccgggcgg atgcagatct ccatccggtg caggccgaac tggtcgaagg
      841 ccgcgtccgt cgccatcgcc acggcggtcg gagtgatccc cctgcctgcg aaccgctcac
      901 tcacccagta gccgatggtc gccgatccca gcgagccgcg tgccacgccc cagatgttga
      961 gctgcccggc gatctcgccg tcgtactcca tcacgaacgg gtacccggcg ccgtcccggt
     1021 gctgctgcag cagacggcgg atgctgacgc gcatgtcgaa ggtcgcgacg cccccgggga
     1081 tcgtcgcctc ccacggccgc agccaggagc ggttggagag cagctcgcgc tgcagcgtgc
     1141 gcgcatccct gctgcggacc agtctcagac ccaccgggcc gtgctgcatc gggtcgaagc
     1201 cgtcgatcgc catgtcggtc cgtcggctac aggccgccgg cgaagtcctt cagccaggga
     1261 cgcaggtccg cccccaggtc gtcgcggtcg acggccagct gcacgatcgc cttgatgtag
     1321 tcgacgcggt cgccggtgtc gtagcgacgg ccgcggaaca ccacgccgac cacgccgggc
     1381 ccgtcaggat cggtggcgag ttcctgcagg gcgtcggtca gctggatctc cccgcccttg
     1441 ccgggctgcg tgcgctccag cacctcgaag acggccgggg gcagcacgta ccggccgatg
     1501 acggcgaggt cggacggggc gtcctcggcg ctcggcttct cgacgagccc ggtcactcgg
     1561 acggcgccgt ccaggtcgtc gccgtcggtc gcagcgacgc cgtagaggtg gatgctctcg
     1621 gcgggaacct gcatgagcgc gatcaccgcg gccccggtgc gctcgtgcgc ggcgatcatc
     1681 tcggtgagca gcgggtcgcg ctcgtcgatg aggtcgtcgc cgagcagcac cgcgaaggag
     1741 ctgtcgccca cgtgggtcct ggcccgcagc acggcatgcc cgaggccctt cggctcgccc
     1801 tgccggacga agtggatgtc ggccaggtcg ctcgacttca tcactcgctc caggcggccg
     1861 tggtcgccct tctgctgcag cctgacctcc agctcgggca ccgagtcgaa gtggtcggag
     1921 atggcgttct tgttccgccc gatgatgacc aggatgtcgt cgatcccggc gctcacggcc
     1981 tcttccacga cgtactggat cgcgggcttg tcgacgacgg ggagcatctc cttgggcatc
     2041 gccttcgtcg cagggaggaa ccgggttccg agccctgcgg cgggaatgac cgccttcatc
     2101 ttgctcatcc ggtcagcata ccggcggcat ccgctcgcac ggcgggcccg caggcggccc
     2161 gccgtatgat cggagcatgc ccggcgacga ggaacagcag aagcgagcac tgcgtgcaga
     2221 gctgcgcgaa cgacggcagc tgctgtccga ttcgcagcgc gaatcggctt cggacggcgt
     2281 ggccgctcag cttcatgagc tcatccgctc ccatggcgcc cgctcggtgt catgcttcct
     2341 gtccacgacc acggagcccg ggacgcgccg attcgtgcgc gaggccgtcg ccaccggcat
     2401 ccgcgtgctg ctgccgatca cccgggcgga cggactgctg gactgggcgg tcgcgaacga
     2461 cagcgatgag gtgtccgagg gcctgtacgg cctcccggag ccgaccggcg aggtgctcgg
     2521 cccgatcgcc gtgaacgacg tggacctgat gatcatcccc gctgccgcga tcgaccgcac
     2581 cggcacccgg ctgggctggg gacgcggcta cttcgacaag acgatcggct ccatggagaa
     2641 gtgcccgccg gtttacgccg tcatctatga ttccgaagta ctcgattcgc tgccgcgcga
     2701 ggtccacgac cagccggtgg atggcatcgt caccccgacg cagaccatcg ccctgtcgcc
     2761 cgcgcgccac tgagacgccc cgcaccgagc ccgaaggaat caggaactcc catgcccacc
     2821 tacgcctacg cctgcacggc ctgcgaccac cgcttcgacg ccgtgcagag cttctcggag
     2881 gactccctca ccgtctgccc tgagtgcggc ggcgcgttgc gcaagcagta cggcaccgtg
     2941 ggtgtgacct tcaacggctc gggcttctat cgcaccgact cgcgcggaaa tactggtgcg
     3001 acgacgggaa gtccggcatc atccaaggtg gattcttcga ccggctccaa tccggcgcct
     3061 gcgaagaccc cggcctcgag ctgaggtcga acacaactcc agggggagtc catgttccag
     3121 ggattcaagg aattcatcgc caagggcaat gtcatcgacc ttgccgtcgc agtcgtcatc
     3181 ggcgcggcct tctcggccat catcgcggcc ctcgtcgaca acctcatcaa cccgctcatc
     3241 ggcctgatct tcaacgccca ggacctcaac cacgtcaagc tcggcgtgat cgggatcggc
     3301 gccatcatcg gtgcggtcat caacttcctg gccgtcgcac tgatcgtgta cttcgtgttc
     3361 gtcctgccga tgaacaagta caaggagcac caggccgcca agaacccggc cgtcgaggag
     3421 gagaccctcc ccagcgagca ggaactgctc atcgagatcc gcgacgcgct gcgcaagaac
     3481 gcggccggct gacctcggcc atcacgacga cgagcgcctc gtcccttcgg gggcggggcg
     3541 ctcttctgca tcccgccgcg ctcacccacc gtgctcagta gtgcgggggc acctcgcgca
     3601 gcatccgatc gtcgttgggg ccgtggtcgc cctcggtccc ggcatggcgc tcctcgcgca
     3661 cgtgcggttc ggcggtggtc cccgccacgg gagtcaggcg tgcgcgccgc gcgccgggga
     3721 cgcgttcgat gtgctgacgg tcctcgtcac tcatcgacgt cgaacggctg cgtgtcgttg
     3781 tcggcgcgga cagcgtcctc ctcgatgccg aggacggtgg cgatgcgccg tgcgaccgtc
     3841 gcggggtcgc tgtagagatc gaacgcgtgc acgcgcacat agtgccagcc caggcgccgc
     3901 agcacctgcg gacgcagccg cagactctcc cgcagcgact cgtcgcggga ctccgggtcg
     3961 gactcgatca cgaccgcccg tccgccgtgc tgcgcgacca gcggcagcag
//
LOCUS       DATMSX010000210         3633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_31261,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000210 DATMSX010000000
VERSION     DATMSX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31261"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN041_06550"
     CDS             complement(<1..246)
                     /locus_tag="VN041_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28742.1"
                     /translation="MAFALRRARQHAGRLSLIALVVALLVAGIGGIDAVAERMLAEGA
                     SRVFTDAEPGARTIRVVADEATDATAQDEAVRREISAA"
     gene            complement(364..439)
                     /locus_tag="VN041_06555"
     tRNA            complement(364..439)
                     /locus_tag="VN041_06555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(404..406),aa:Ala,seq:ggc)
     gene            558..1349
                     /locus_tag="VN041_06560"
     CDS             558..1349
                     /locus_tag="VN041_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28743.1"
                     /translation="MVRNLLRLVWLLLSAWLIVWVLEGLFGVDQRHSVLPFQGDGSSR
                     PALFIGVAWGILPMLGIFSPAGPGRRRPRHDDRIAVAKVIAVDATGTTINDVPQYDIF
                     VRVTPDSGEDFIGQLRMLITPDEQSSLVIGAPLPVRFSSVKPDAVALADPLDPAVRQA
                     LLDWRIAKGLIDPRQVRARTSGITSPADILSVRPTGKRLEGQSELALTVLISPEGQTP
                     WEAETTLFAYPQAIGRLQVGSPVWAMYRREDPKTVAITIEKETVA"
     gene            1351..1884
                     /locus_tag="VN041_06565"
     CDS             1351..1884
                     /locus_tag="VN041_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28744.1"
                     /translation="MLDTIFWLLNFPASNGYAMVFIAAFGSTGLAMLAFGGTRGGRSR
                     LQALREQEGLATPTASAGPAVLARARQIVFRIVFAVVLGSGALGLLALIGVPVTNAYI
                     HANGIETTGTMDSGDWVTFTTEDGTEYTLANDFFTPSVYPDAHASVGWGDAPVTVRYL
                     PGHPQAFVIDSTPASGG"
     gene            1960..2385
                     /locus_tag="VN041_06570"
     CDS             1960..2385
                     /locus_tag="VN041_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HWU28745.1"
                     /translation="MSTDDAGRPLTNWHTSHTEQLTRGQRAADRLRNGMGSWTFIAAF
                     VLFMLAWAAVNLTAVAWDPYPYILLNLFLSMLAGLQGAILLIAAKRQDAIAAALAQHD
                     YDTNLAAKREIEELMEINRTQLQLLRELRSAAGEAPAAR"
     gene            2472..2789
                     /locus_tag="VN041_06575"
     CDS             2472..2789
                     /locus_tag="VN041_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28746.1"
                     /translation="MSEPTPGYLPEDPDLDDDAEPGTRVVDGEEVLDPDADPDLIDSA
                     EADRLAADGDDAADVGDVPAGGAGADSGAVEAVQVDPEDEAEIPDISETNDEPGVGTR
                     RPD"
     gene            complement(2815..3192)
                     /gene="rsfS"
                     /locus_tag="VN041_06580"
     CDS             complement(2815..3192)
                     /gene="rsfS"
                     /locus_tag="VN041_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWU28747.1"
                     /translation="MQSPDNALEMLQIAADAADSKGGEDLVALNVSGPLPLVDIFLLV
                     TGNSERNVAAIADEIEDQLHEAGHRRVRREGRAEARWVLLDFGDLIVHVFHSEERVYY
                     GLERLWKDCPVVPIELTTKVPGE"
     gene            complement(3192..>3633)
                     /locus_tag="VN041_06585"
     CDS             complement(3192..>3633)
                     /locus_tag="VN041_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28748.1"
                     /translation="ATAPFDALVGGTSSSAHHGTSTALIFTPSPGVGSLSGPIASTGE
                     ILVTGTYALPDGLGSQGHAHGTTDGKDIDAVLIDGELAPASSPTPIAASAAVSTSKPA
                     GEVIRPPAPDKGNKLMLTLAIVAGTLAVVLGATLVIAFTTGVFG"
BASE COUNT          560 a         1274 c         1254 g          545 t
ORIGIN      
        1 tgcggcggag atctccctgc gcacggcctc gtcctgcgcg gtggcgtcag tcgcctcgtc
       61 ggcgaccacg cggatcgtgc gggcgccggg ctcggcatcc gtgaagacgc gggaggcgcc
      121 ttccgccagc atccgctcgg cgaccgcatc gatgccaccg atccccgcca ccagcagcgc
      181 gaccaccagg gcgatcagcg acagacgacc ggcgtgctgt cgggcccgcc tcagtgcaaa
      241 cgccattgct cctcatcaca ccacgcaatg ctgaacatcc tcgcgtggac gggacggcga
      301 cgcaacggct cgtgcgtagg gccgggaacg gaagaagccc gcgatctctc gcgggcttct
      361 cgatggtgga cctgagggga ctcgaacccc tgaccccctg catgccatgc aggtgcgcta
      421 ccagctgcgc cacaggccca tcttcgctgc caggaccgga gccccggcaa cctgttcagc
      481 atactacgga gtccgcagcg ctcgcgaatc gtgccgattc gccgggtgtg tcgccgagca
      541 gccgatagcc tgggcgcgtg gtccgcaacc tcctccgcct cgtctggctg ctgctctccg
      601 cctggttgat cgtctgggtg ctcgaggggt tgttcggcgt cgatcagcgt cattccgtcc
      661 ttccgttcca gggagacgga tcgagccgtc cggcactgtt catcggagtg gcgtggggca
      721 tcctgccgat gctcgggatc ttctccccgg caggacccgg tcgcaggcgc ccgcgacacg
      781 acgatcggat cgccgtcgcc aaggtcatcg ccgtcgacgc caccggcacc acgatcaacg
      841 acgtgccgca gtacgacatc ttcgtgcgcg tgaccccgga ctccggcgag gacttcatcg
      901 gacagctgcg gatgctgatc acgcccgacg agcagtcctc tctcgtgatc ggggcgcccc
      961 tgccggtgcg cttctcctcg gtgaagcccg acgcggtcgc cctggccgat ccgctggatc
     1021 ccgcggtgcg ccaggctctg ctggactggc ggatcgcgaa gggcctgatc gacccgcggc
     1081 aggtgcgggc caggacgagc ggcatcacct cccccgcgga catcctctcg gtccgcccca
     1141 cggggaagcg cctcgagggg cagagcgaac tcgccctcac cgtgctcatc agccccgagg
     1201 gacagacacc gtgggaggcg gagacgacgc tgttcgccta ccctcaggcg atcggacgcc
     1261 tgcaggtcgg ttcaccggtg tgggcgatgt accgacgcga ggacccgaag accgtcgcga
     1321 tcacgatcga gaaggagacg gtggcctgag atgctcgaca cgatcttctg gctgctgaac
     1381 ttccccgcat ccaacggcta tgcgatggtc ttcatcgccg cgttcggcag cacgggcctc
     1441 gccatgctcg ccttcggcgg cacacgcggc ggccgatcgc ggcttcaggc gctgcgcgag
     1501 caggagggtc tggcgacccc gacggcatcc gcgggtccgg cggtgctcgc acgggcgcgg
     1561 cagatcgtct tccgcatcgt cttcgccgtc gtgctcggca gcggagctct cggcctgctg
     1621 gcgttgatcg gggtgccggt cacgaacgcg tacatccacg cgaacggcat cgagaccacc
     1681 ggcacgatgg actcgggcga ctgggtgacg ttcaccaccg aggacggcac cgaatacacc
     1741 ctcgcgaacg acttcttcac gccgtcggtg tacccggacg cgcacgcgag cgtcggctgg
     1801 ggagacgccc ccgtcaccgt gcgctacctg cccgggcatc cgcaggcgtt cgtgatcgac
     1861 agcactccgg catccggcgg ctgaaccgcg ggccacgctc ccccggggcc ttcccgatcg
     1921 actagatcgg gacgaccggt gcacgcctat atttctcaca tgagcactga cgacgcggga
     1981 cgtcccctga cgaactggca cacctcgcac accgagcagc tgacccgcgg gcagcgagcc
     2041 gccgaccgac tgcggaacgg gatgggctcg tggacgttca tcgccgcatt cgtgctgttc
     2101 atgctcgcct gggcggccgt caacctgacc gccgtcgcct gggatccgta cccgtacatc
     2161 ctgctgaacc tgttcctcag catgctcgcg ggactccagg gcgcgatcct gctgatcgcg
     2221 gcgaagcggc aggacgccat cgccgcggcg ctcgcgcagc acgactacga caccaacctc
     2281 gccgcgaagc gggagatcga ggagctgatg gagatcaacc ggacgcagct gcagctgctg
     2341 cgcgagctgc gctcggcagc cggcgaggct cctgcggcgc gctagccgga ccgcggtcgc
     2401 gcgtcaaccc gctcgtcacg cctgccgctc ggacgtaacg tcaccggcat ccgttctgag
     2461 aggagcacgc catgtccgaa ccgacacccg gctatctgcc cgaggacccc gacctcgatg
     2521 acgacgcgga acccggcacg cgggtcgtcg acggcgagga ggtcctggac cccgatgccg
     2581 accccgacct gatcgacagc gccgaggccg accgactggc cgccgatggc gacgacgcgg
     2641 ccgatgtcgg cgacgtgccg gccggtggtg ccggtgctga cagcggcgcg gtcgaggccg
     2701 tgcaggtgga cccggaggac gaagccgaga tcccggacat ctccgagacc aacgacgagc
     2761 cgggtgtcgg gacgcgtcgt cccgactgaa ccgcgggggt tactccccgg ggagttactc
     2821 cccggggacc ttcgtggtca gctcgatcgg aacgaccggg cagtccttcc agagccgctc
     2881 gaggccgtag tagacgcgct cctcggagtg gaacacgtgc acgatgaggt cgccgaagtc
     2941 cagcagcacc cagcgcgcct cggcgcgtcc ctcccggcgc acgcgcctgt gcccggcctc
     3001 gtgcagctgg tcctcgatct catcggcgat cgcggcgacg ttgcgctcgc tgttaccggt
     3061 gacgagcagg aagatgtcga ccagcggaag cgggccggag acgttgaggg cgacgagatc
     3121 ctcgccgccc ttcgagtccg ccgcgtctgc ggcgatctga agcatctcga gggcgttgtc
     3181 gggggactgc atcaaccgaa gactcctgtg gtgaaggcga tgacgagggt ggcacccagc
     3241 acgacggcca gggttcccgc gacgatcgcc agggtgagca tgagcttgtt gcccttgtcg
     3301 ggtgccggcg ggcggatgac ctcaccggcg ggcttggagg tgctgacggc ggcgctggcc
     3361 gcgatcggcg tgggcgacga ggcgggcgcg agctcgccgt cgatgagcac ggcgtcgatg
     3421 tccttgccgt ccgtcgtgcc gtgcgcgtgc ccctgcgatc ccagcccgtc gggcagggcg
     3481 taggttccgg tgacgaggat ctccccggtc gaggcgatcg gccccgagag cgagccgacg
     3541 ccgggcgacg gggtgaagat cagcgcggtg gaggtgccgt ggtgcgcgct ggacgacgtg
     3601 ccgccgacga gggcgtcgaa cggcgcggtg gcc
//
LOCUS       DATMSX010000211         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_144862,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000211 DATMSX010000000
VERSION     DATMSX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144862"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3917)
                     /locus_tag="VN041_06590"
     CDS             complement(<1..3917)
                     /locus_tag="VN041_06590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWU28749.1"
                     /translation="MSDVLDRFTPATQDWFRGAFDAPTNAQAGAWEAISAGRNALVVA
                     PTGSGKTLSAFLWAIDSVFRERVEGADADGTPRTRILYISPLKALGVDVERNLRSPLI
                     GIGQSARRLGLPVPEVTVGVRSGDTTSSDRRKLVSAPPDILITTPESLYLMLTSRAGE
                     TLRGVHTVIIDEVHAVAATKRGAHLAVSLERLDALRAARGAESPAQRIGLSATVRPID
                     EVARFLGGSAPVDIVAPRAVKTFELGVVVPIEDMRNPPPPPGAATSNDDPAEYTEVTG
                     SVWPHVEEAIVDKVLENRSTIVFANSRRLAERLTGRLNEIYSERIGVALPDNASPPAA
                     MMAQAGSTAGADPVLAKAHHGSVSKEQRAIVEEELKSGVLRCVVATSSLELGIDMGAV
                     DLVIQVEAPPSAASGLQRVGRAGHQVGEISRASLFPKHRGDVLHTAIVTERMLKGQIE
                     AIAVPRNPLDILAQQTVAACALDPISVEEWFETVRRSAPFQTLPRSAYEATLDLLAGK
                     YPSDEFAELRPRVVWDRDAGTLTGRPGAQRIAVTSGGTIPDRGLFGVFVAGETTGARV
                     GELDEEMVYESRVGDVFTLGTTSWRIAEITHDRVNVIPAYGQPGKVPFWHGDGIGRPF
                     ELGEALGRFSREVSTAEPSKAQQRLIDAGLDDMARANLIGYLTEQREATGTLPTDRTL
                     TVERGHDEVGDWRVILHSPYGMKVHAPWALAINARVRERLGVEGSAVASDDGIIVRIP
                     DADAEPPGADLFVFDADELEQIVTAEVGGSALFASRFRECAARALLMPRMNPNKRTPL
                     WQQRQRSAQLLEVARRHPTFPVILETLREVLQDVYDLPSLRRLITDISERRIRLVETE
                     PAQPSPYARDLLFGYVGAFMYEGDSPLAERRAAALSVDPALLGELLGTVEMRELLDPD
                     VIAQFEREAQRLDPQRRARGVEGVADLLRMLGPLDAEEVAARLEPSTDGGESSASPEQ
                     ASALLNELVTARRAVPITIAGHARVAVIEDAGRLRDALGAALPTGIPVAFLEPVADPL
                     GDLVSRHARTHGPFTTDAVATRFGIGAAVARHTLQRLESAGRLTSGYFLPADDADPGG
                     AKEWCDAEVLRRLRMRSLAAIRGSVEPVSPQAYARFLPDWQHLTRPLEGIDGVLSVIE
                     QFAGVPIPASAWESLVLPSRVRDYAPALLDELTAAGEVVWAGHGSLPGRDGWVSLHPA
                     DLAPFTLPVPEESPGAGSLEEKVLAVLRLSGASFAGALRAMTDAENERSVLDALWTLT
                     WQGHVTNDTFAPVRALIAGGSQAHKTARRAPRTRTYRGVSLTRP"
     gene            3974..4588
                     /locus_tag="VN041_06595"
     CDS             3974..4588
                     /locus_tag="VN041_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28750.1"
                     /translation="MEEAEFTRLYGQWAPRAPEDVRDFFDGYGGTWWIAGGWALQAFT
                     GVERHHEDIDPGILRAELPLLRAHVGDRLNLWAAFSGALKPVASDDPDDLPDGCGQLW
                     GRRSAADPWEYDILLGPGTTDEWQYRRDPSVRMPMADALWQRDGIRFLQPEIQLLYKA
                     RGLRDKDRADFDATLPFLDGRRRAWLADALRRTIPGHPWLAALG"
     gene            4627..5037
                     /locus_tag="VN041_06600"
     CDS             4627..5037
                     /locus_tag="VN041_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HWU28751.1"
                     /translation="MAHTTNYTNTFIEVSEDCRAEHGEEPPIAENLSIAALHYRLLIE
                     EPYTRTSDDVLFETHALRKGIDPHDEAAREEFFSKGQACLRSSPLGKRYGWGTHHDDE
                     GRVALIPRESEEYARLAADAELAHTRAMRSSRAK"
     gene            complement(5041..5223)
                     /locus_tag="VN041_06605"
     CDS             complement(5041..5223)
                     /locus_tag="VN041_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWU28752.1"
                     /translation="MTPLVRPRQDRVIAGVCSAVARRFDISPTTVRVITVLAVLFAGL
                     SIWAYVLLWIIVPNEL"
     gene            complement(5263..>6776)
                     /locus_tag="VN041_06610"
     CDS             complement(5263..>6776)
                     /locus_tag="VN041_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28753.1"
                     /translation="YLPQRLTLDTDRRVAELLGIAAQLDAVRAITAGDVDPAHFDAVG
                     DDWDIEARAEVSLAEAGLDPSFLDRTVGELSGGESVLVAIAGIRLRRAPITLLDEPTN
                     NLDRVARGRLADMVRGWKGALIVVSHDIALLELMDDTAELYGSSLSVFGGPYSEWRAW
                     LDAEQEAARQDERDATQAFKKEKRQRIEAETKLAHRASFAKKAYENKREPKIVMNGRK
                     MAAQVSAGRLRTEVAGKEETARAAMDAAGRRVRDDASMKIELPDPGVSNSRRIATLGD
                     GTRSWVVQGPERVALIGRNGVGKTTLLRRLVADADLTGGNASASSGNVHLARMDPEDS
                     SAAGAHSRVEGADAAASAFPINPELVAEAHTDRIGYLSQRVDGLDETASVVENIAAAA
                     PHVPDKELRNRLARFLIRGATMDRPVGALSGGERFRVALAKLLLADPPPHLVVLDEPT
                     NNLDLDTADQLVQALLAYRGAVLVVSHDDVFLQRLELDLTLELDAEGQLAQIG"
BASE COUNT          976 a         2406 c         2414 g          980 t
ORIGIN      
        1 ggtcgcgtca gcgagacgcc ccggtacgtg cgggtgcgcg gtgcgcgtcg cgccgtcttg
       61 tgcgcctgcg agcctccggc gatcagcgcc ctcacgggtg cgaacgtgtc gttcgtgaca
      121 tggccctgcc acgtcagggt ccagagggcg tcgagcacgg accgctcgtt ctcggcgtcc
      181 gtcatcgcac gcagcgcgcc cgcgaacgac gctccgctga gccgcagcac ggcgagcacc
      241 ttctcctcga gcgatcccgc accgggcgac tcctcgggga ccggcagcgt gaacggcgcg
      301 aggtcggccg gatgcagcga gacccagccg tctcggcccg ggagcgaccc atggccggcc
      361 catacgacct cgcccgccgc ggtcagctcg tcgagcagcg ccggcgcgta gtcgcgcacc
      421 cggctcggca gcaccagcga ctcccacgca ctggcgggga tcggaactcc ggcgaactgc
      481 tcgatcacgc tgagcacgcc gtcgatgccc tccagcgggc gggtcagatg ctgccagtcc
      541 ggcaggaacc gcgcataggc ctgtgggctg acaggctcga cgctgccgcg gatcgcggcg
      601 agcgagcgca tccgcagccg gcgcagcacc tcggcgtcgc accactcctt cgccccgccc
      661 gggtcggcgt cgtcggcggg caggaagtaa ccgctggtca gtcgtcccgc cgattccagc
      721 cgctggaggg tgtgccgcgc gacggcggcc ccgatgccga agcgcgtggc gacggcatcc
      781 gtcgtgaacg gaccgtgggt gcgggcatgc cgcgaaacga ggtcgccgag ggggtcggcg
      841 accggctcca ggaacgcgac ggggatgccc gtgggcagag ctgcgccgag tgcgtcgcgc
      901 agacgcccgg catcctcgat cacggcgact cgggcgtggc cggcgatcgt gatcgggacg
      961 gcgcgacggg cggtgacgag ctcgttgagg agagcggatg cctgctcggg cgaagcggag
     1021 ctttcccctc cgtccgtcga gggctccagc cgcgcagcaa cctcctcggc atccaggggt
     1081 ccgagcatcc gcagcaggtc ggcgacgccc tccacccccc gtgcgcggcg ctgcgggtcg
     1141 aggcgctgcg cctcccgctc gaactgcgcg atgacgtcgg ggtcgagcag ctcgcgcatc
     1201 tcgaccgtgc cgagcagttc gccgagcagc gcgggatcga ccgacagtgc ggcggcgcgg
     1261 cgctcggcca gcggcgagtc gccctcgtac atgaacgcgc cgacatagcc gaagagcaga
     1321 tcgcgcgcat acggcgaggg ctgcgcgggc tcggtctcga ccaggcggat gcgccgctcg
     1381 gagatgtcgg tgatgagtcg gcgcagcgac ggcaggtcgt agacgtcctg cagcacctcg
     1441 cgcagggtct ccaggatcac agggaaggtc gggtggcgcc tggcgacctc cagcagctgc
     1501 gccgagcgct ggcgctgctg ccacaggggc gtgcgcttat tggggttcat ccgcggcatc
     1561 agcagggcgc gggccgcgca ctcgcggaag cgcgaggcga acagcgccga cccgccgacc
     1621 tcggcggtga cgatctgctc cagctcgtcg gcgtcgaaca cgaagaggtc cgccccgggc
     1681 ggctcggcat cggcatccgg aatgcgcacg atgatgccgt cgtcgctcgc gaccgccgat
     1741 ccctccactc cgagccgctc ccgcacgcgt gcgttgatcg ccagcgccca cggcgcgtgc
     1801 accttcatgc cgtacgggga atgcaggatg acccgccagt cgccgacctc gtcgtggccg
     1861 cgttcaacgg tgagggtgcg gtcggtgggc agcgtgccgg tggcctcgcg ctgctcggtc
     1921 aggtagccga tcaggttggc gcgcgccatg tcgtccaggc ccgcgtcgat caggcgctgc
     1981 tgcgccttcg agggctccgc cgtggagacc tcgcgggaga agcggccgag ggcctcgccc
     2041 agctcgaagg gccgcccgat gccgtcaccg tgccagaacg gcaccttgcc cggctggccg
     2101 tacgcgggga tgacgttgac ccggtcgtgg gtgatctcgg cgatccgcca gctggtcgtg
     2161 ccgagcgtga acacgtcgcc gacgcgcgac tcgtagacca tctcctcgtc gagctcgccc
     2221 acacgggcgc ccgtggtctc gcccgccacg aagacgccga acagtccgcg gtcggggatc
     2281 gtcccgccgc tggtcaccgc gatgcgctgc gcaccgggtc ggccggtcag cgtcccggca
     2341 tcccgatccc acaccacccg ggggcgcagt tctgcgaact cgtcggacgg gtacttgccg
     2401 gcgagcaggt cgagcgtcgc ctcgtacgcc gagcggggga gagtctggaa cggcgcgctg
     2461 cgccgcacgg tctcgaacca ctcctcgact gagatcggat cgagcgcgca ggccgcgacg
     2521 gtctgctgag cgaggatgtc gagcggattg cgcggcacgg cgatcgcttc gatctgtccc
     2581 ttcagcatcc gctcggtgac gatggccgtg tgcagcacgt cgccgcgatg cttgggaaag
     2641 agggatgccc ggctgatctc gccgacctgg tgcccggcgc ggccgacgcg ctgcaggccg
     2701 gatgccgcgg acggcggcgc ctccacctgg atcaccaggt cgaccgcacc catgtcgatg
     2761 ccgagctcca ggctgctcgt cgccacgacg cagcgcagga cgccggactt caattcctcc
     2821 tcgacgatcg cgcgctgctc cttggaaacc gagccgtgat gggccttcgc gagcacggga
     2881 tcggcgccgg cggtcgaccc cgcctgcgcc atcatggcgg ccggcggaga ggcattgtcg
     2941 ggaagggcca ccccgatccg ctccgagtag atctcgttga gtcgcccggt gagccgctcc
     3001 gccagccggc gcgagttggc gaacacgatg gtcgaccggt tctcgagcac cttgtcgacg
     3061 atcgcctcct cgacgtgcgg ccagacggag ccggtcacct cggtgtactc ggcgggatcg
     3121 tcgttcgagg tcgctgcgcc gggcggcggg ggagggttgc gcatgtcctc gatcggcacc
     3181 accacgccga gctcgaacgt cttcacggcg cgcggcgcca cgatgtcgac cggcgcggaa
     3241 ccgccgagga agcgggcgac ctcgtcgatc ggtcggacgg tggccgacag gccgatgcgc
     3301 tgcgccggag attccgcgcc gcgcgctgca cgcagggcat ccagcctctc caggctcact
     3361 gcgagatgtg cgccgcgctt ggtggcggcc accgcgtgca cctcgtcgat gatcaccgtg
     3421 tgcacgccgc gcagtgtctc gccggcgcgg ctggtgagca tcaggtacag cgattcgggc
     3481 gtcgtgatga ggatgtcggg tggggcgctg accagcttgc ggcggtcgct cgacgtggtg
     3541 tccccggaac gcacgccgac cgtgacctcg ggcacgggca gacccagccg ccgcgcggac
     3601 tgcccgatgc cgatcagggg cgagcggagg ttgcgctcca cgtcgacgcc cagcgccttc
     3661 agcggggaga tgtagaggat gcgcgtgcgc ggcgtgccgt cggcatccgc tccctccacc
     3721 cgttctcgga agacgctgtc gatcgcccag aggaacgcgg acagcgtctt gcccgacccg
     3781 gtcggcgcga cgacgagcgc gttgcgtccc gcggagatcg cctcccaggc acccgcctga
     3841 gcgttcgtcg gcgcgtcgaa cgccccgcgg aaccagtcct gggtcgcggg ggtgaagcgg
     3901 tcgagcacat cgctcatccc tccatcatgc ggcaggccgc cgacactctg gcggcctccg
     3961 ataccgtgaa cggatggaag aggccgagtt cacgcgtctg tacgggcagt gggcgccgcg
     4021 cgcgcccgag gacgtccggg acttctttga cggctacggc ggcacgtggt ggatcgccgg
     4081 cggctgggcc ctgcaggctt tcacgggtgt cgagcgccac cacgaggaca tcgatcccgg
     4141 catcctccgc gccgagctgc ctctcctgcg tgcgcatgtc ggcgatcgtc tgaacctgtg
     4201 ggcggcgttc tcgggtgcgt tgaagccggt agcatccgac gatccggacg acctcccgga
     4261 cggatgcggt cagctctggg ggcgccgcag cgccgcggat ccttgggagt acgacatcct
     4321 gctcgggccg ggaacgacgg acgaatggca gtaccggcgc gacccgagcg tccggatgcc
     4381 gatggccgac gccctctggc agcgcgacgg catccgcttt ctgcagcccg agatccagct
     4441 gctctacaag gcgaggggac tgcgcgacaa ggatcgcgcg gacttcgacg cgaccctgcc
     4501 gttcctggat ggtcggcgac gcgcctggct cgcggatgcg ctgcggcgga cgatccccgg
     4561 gcacccgtgg ctcgcggcgc tgggctgatc ctcgacaatg tcgccggggt gggggatgct
     4621 ggccgcatgg cgcacacgac gaactacacg aacacgttca tcgaggtctc ggaggactgc
     4681 cgtgccgagc acggcgagga gcctccgatc gccgagaacc tctccatcgc cgcgctgcac
     4741 taccgactgc tcatcgaaga gccgtacacg cgcacgtccg acgacgtgct gttcgagacg
     4801 catgcgctgc gcaagggcat cgacccgcac gacgaggcgg cacgtgagga gttcttctcg
     4861 aagggtcagg cctgtctgcg ctcgtccccg ctgggcaagc gctacggctg gggcacacac
     4921 cacgatgacg aggggcgcgt ggcgctgatc ccccgggagt ccgaggagta cgcgcggctc
     4981 gccgccgacg cggagctcgc ccacaccagg gccatgaggt ccagccgggc gaagtgagcc
     5041 tcagagctcg ttcggcacga tgatccagag cagcacgtag gcccagatcg acaggcccgc
     5101 gaacagcacg gcaagcaccg tgatcacgcg cacggtcgtc gggctgatgt cgaatcgtcg
     5161 cgccacggcc gagcagacgc cggcgatcac gcggtcctgg cgggggcgga cgagaggagt
     5221 catacctccc atcctgcccg ccaggaccgc gccgcggaac cctcaaccga tctgggccag
     5281 ttggccttcg gcatccagct ccagcgtcag gtcgagttcc agtcgctgca ggaatacgtc
     5341 gtcgtggctg acgacgagca ccgctccgcg gtaggcgagc agcgcctgaa ccagctgatc
     5401 ggccgtgtcg aggtcgaggt tgttggtcgg ctcgtcgagc accacgaggt gcggcggggg
     5461 atcggcgagc agcagcttcg ccagggccac ccggaacctc tcaccgcctg aaagggcgcc
     5521 gacggggcgg tccatcgtgg ccccgcggat caggaaccgg gcgagccggt tgcgcagctc
     5581 cttgtcgggc acgtgcggag ccgccgccgc gatgttctcc accacggacg ccgtctcgtc
     5641 gagcccgtcc acacgctggg acaggtagcc gatccggtcg gtgtgcgcct ccgcgaccag
     5701 ctccgggttg atcgggaacg cggatgccgc ggcatccgca ccctccacgc gggaatgtgc
     5761 gccagcggcg gacgaatcct ccggatccat ccgcgcgagg tgcacattcc cgctcgaagc
     5821 gctcgcgttc ccgccggtga ggtcggcatc ggccaccagc cgccgcagca gcgtggtctt
     5881 cccgacgccg ttgcgcccga tcagcgccac gcgctccggc ccctgcacca cccacgaccg
     5941 cgtgccgtcg ccgagtgtgg cgatgcgtcg cgagttcgag acgcccgggt cgggcagctc
     6001 gatcttcatc gacgcgtcgt cgcgcacacg gcggccagcg gcgtccatcg cggcgcgtgc
     6061 cgtctcctcc ttgcccgcga cctcggtgcg cagccggccg gccgagacct gcgcggccat
     6121 cttgcgaccg ttcatgacga tcttcggctc gcgcttgttc tcatacgcct tcttcgcgaa
     6181 gctcgccctg tgcgcgagct tggtctccgc ctcgatgcgc tgccgcttct ccttcttgaa
     6241 cgcctgcgtc gcatcgcgct catcctgccg ggcggcttcc tgctcggcat ccagccatgc
     6301 ccgccactcg gagtaggggc cgccgaacac gctcaggctg ctgccgtaga gctcggcggt
     6361 gtcgtccatc agctccagca gcgcgatgtc gtgactcacc acgatcagcg cgcccttcca
     6421 gccgcggacc atgtccgcca ggcgaccccg cgccacgcgg tcgaggttgt tggtcggctc
     6481 gtcgagcagg gtgatcggcg cacggcgcag ccggatgccg gcgatcgcga ccagcacgga
     6541 ctcgccgccg gacagctcgc cgaccgtgcg atcgaggaac gacgggtcga gcccggcctc
     6601 ggcgagggac acctcggctc tcgcctcgat gtcccagtcg tcgccgacgg cgtcgaagtg
     6661 cgccgggtcg acgtcgccgg ctgtgatcgc gcgcacggcg tcgagctgag cggcgatgcc
     6721 gagcagctcg gcgacgcggc ggtcggtgtc gagcgtgaga cgctgcggca ggtagg
//
LOCUS       DATMSX010000212         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3235,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000212 DATMSX010000000
VERSION     DATMSX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VN041_06615"
     CDS             <1..251
                     /locus_tag="VN041_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWU28754.1"
                     /translation="DAIIAAWVGLEETAADSGVGRGVSETPAEFTLRILLRRPGIEHP
                     AQQLLAVYEGVRFGGHRGTEQDRALASRALALIEEGWR"
     gene            248..778
                     /locus_tag="VN041_06620"
     CDS             248..778
                     /locus_tag="VN041_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28755.1"
                     /translation="MRWLHALLMAAGVGFLAGIGLAVLRVQVEFVWGWSVLVAALVLL
                     IGLRLPDDPRMDAPRLPDPPDYIGSDVSRLAWAINMHTDTANEAVTRRLRATLRRRLR
                     QHGMDVDDPRQASAVDRMLGDGLWGRLNGRRTTVADVRAALAAAERLGGDRGDDAARR
                     NIAQDPERPSQRESTS"
     gene            775..1740
                     /locus_tag="VN041_06625"
     CDS             775..1740
                     /locus_tag="VN041_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU28756.1"
                     /translation="MNFADIAELGQRIRASVATAVVGMDDPLEVALATILAGGHVLFE
                     DVPGLGKTLAARSLASALGLSFRRLQCTPDMLPGDVTGSYVYAPDSGDFVFRPGPIFT
                     GLLLADEINRTTPKTQSAMLEAMAERQVTVEGNSFPLEPPFHVIATANPIEYEGTYSL
                     PEAQLDRFMVRLSVGYLDQQGETEILLGRLRRQQEATHVDPVLAPGQLSQLQQAVERI
                     HVDPDVAAYCVALAAATRQAQHVAVGASPRGSQSLVLLGRALAALDGRDYVRPDDIKR
                     IAVPVLAHRLTLTPQAWAQGVDPTEIVRGLVSSVAVPPSIAAVRG"
     gene            1744..>2087
                     /locus_tag="VN041_06630"
     CDS             1744..>2087
                     /locus_tag="VN041_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28757.1"
                     /translation="MADAAASGGARRAGPPLFLAVFAAAGALIAGVVFSRPDVIAIGV
                     PLALWAAIALTLTGRGGVDVRIERMPDAEEGVLADDIVVDSDAEMVELAVAQAGRTRR
                     RLFVPGRTRVRAT"
BASE COUNT          279 a          743 c          749 g          316 t
ORIGIN      
        1 ccgacgccat catcgcggcc tgggtggggc tggaggagac ggcggcggac tccggggtcg
       61 gtcgcggcgt cagtgagacg cccgcggagt tcaccctgcg gatcctgctg cgcaggccgg
      121 gcatcgagca tccggcgcag cagctgctcg ccgtctacga gggcgtgcgc ttcggcggac
      181 accggggcac cgaacaggat cgtgccctcg cctcccgcgc gctggcgctc atcgaggagg
      241 gctggcgatg aggtggctgc atgcgctgct gatggccgcc ggggtcggct tcctcgccgg
      301 catcgggctg gccgtgctgc gcgtgcaggt cgagttcgtc tggggctggt cggttctcgt
      361 cgccgcgctc gtgctgctga tcgggctgcg gctgccggac gatccgcgca tggacgcacc
      421 gcgccttccg gatccgcccg actacatcgg ctccgacgtc tcgcgtctcg cgtgggccat
      481 caacatgcac accgacaccg ccaatgaagc cgtcacccgc cggctccgcg ccacgctgcg
      541 ccgccggctg cggcagcacg gtatggacgt ggacgacccg cgacaggcat ccgccgtcga
      601 ccggatgctg ggcgacggac tctgggggcg cctgaacggg cgtcgcacca cggtggccga
      661 tgtccgagcc gcgctcgcgg ccgcggagcg gctgggcggc gatcgcggtg acgacgcggc
      721 ccggcggaac atagcgcagg atccggaaag accatcgcag agggagagca catcatgaac
      781 ttcgcggaca tcgccgaact cgggcagcgc atccgcgcgt ccgtggccac cgcggtcgtc
      841 ggcatggacg acccgctcga ggtcgctctg gccacgatcc tcgccggagg acacgtgctg
      901 ttcgaggacg tgcccggcct cggcaagacc ctggccgcgc gcagcctggc atccgcactc
      961 ggcctgtcgt tccgtcgcct gcagtgcacg ccggacatgc tcccgggcga cgtgacggga
     1021 tcgtacgtgt acgcgcccga cagcggcgat ttcgtcttcc ggcctgggcc catcttcaca
     1081 gggctgctgc tggccgacga gatcaaccgc acgactccga agacgcagtc cgccatgctc
     1141 gaggcgatgg cggagaggca agtgaccgtc gagggcaaca gcttccccct cgagcctccc
     1201 ttccacgtca tcgccacggc caacccgatc gagtacgagg gcacgtactc gctgccagag
     1261 gcgcagctcg accggttcat ggtgcgtctc tccgtggggt atctcgacca gcagggcgag
     1321 accgagatcc tgctcggacg tctgcggcgc cagcaggagg cgacgcacgt ggaccccgtc
     1381 ctggcaccag gtcagctgtc gcagctgcag caggccgtcg agcggatcca cgtggacccc
     1441 gacgtcgcgg cgtactgtgt cgcgctggcc gcggcgacac ggcaggctca gcatgtggcg
     1501 gtcggcgcgt ccccgcgtgg atcgcagtct ctcgtgctgc tcggtcgtgc gctggcggcg
     1561 ctggacggac gcgattacgt gcgccccgat gacatcaagc gcatcgccgt gccggtgctc
     1621 gcgcaccgtc tcaccctcac cccgcaggcg tgggcgcagg gtgtcgaccc gaccgagatc
     1681 gtccgcggac tggtctcgtc cgtcgccgtt ccgccgagca tcgccgccgt ccgcggctga
     1741 gtcatggccg acgcagcagc atccggggga gcacgacgtg ccggcccgcc gctcttcctc
     1801 gcggtcttcg ccgctgccgg cgcgctgatc gcgggcgtcg tgttctcgcg gcccgacgtg
     1861 atcgcgatcg gagtaccgct cgccctctgg gcggccatcg ctctcacgct gaccggtcgc
     1921 ggtggcgtcg atgtccgcat cgagcgcatg ccggatgcgg aggagggggt gctcgccgat
     1981 gacatcgtgg tggactcgga tgccgagatg gtggagctgg cggtggcgca ggccggccgc
     2041 acccgccgac ggctgttcgt ccccggccgc acccgggtgc gggccac
//
LOCUS       DATMSX010000213         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135494,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000213 DATMSX010000000
VERSION     DATMSX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135494"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1185
                     /gene="argH"
                     /locus_tag="VN041_06635"
                     /pseudo
     CDS             1..1185
                     /gene="argH"
                     /locus_tag="VN041_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            1246..1890
                     /locus_tag="VN041_06640"
     CDS             1246..1890
                     /locus_tag="VN041_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HWU28758.1"
                     /translation="MAVVVTADIIGSRRLLDRGDAQNALDSAIAQVERDLPVAVEGMR
                     PTVGDEEQAVYPTLEAALGTILLLRLALPDGVEFRYGIGIGEVGTVPSTASDRGISDG
                     PGWWAARSAIEQVETLQRRTAPQARTWVVAHESTDEVSPDAVRWANAYLLSRDELVGA
                     MSERTRRLAYGRCLSRTQSELAASEGITQSAVSQALSSSGAGSVVEGFRLLELT"
     gene            complement(1902..2486)
                     /locus_tag="VN041_06645"
     CDS             complement(1902..2486)
                     /locus_tag="VN041_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28759.1"
                     /translation="MPSLDPFASAMLASAIVVVLLVVLVLSLVGLRRPSTVLAAVGGA
                     LVASALTIVVVLPPAATPLLGVPITVLGFAAATVGGNPFTRRALDIAAGNRVRETEDG
                     GIVILASHDADPAHARTLMRGGTVIGYLERICTVVAIAVGFPEAIAAIIAVKGIGRFP
                     ELAEAEARERFIIGTLASLLWAGAVGTVIRLALV"
     gene            2561..>3761
                     /gene="tyrS"
                     /locus_tag="VN041_06650"
     CDS             2561..>3761
                     /gene="tyrS"
                     /locus_tag="VN041_06650"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWU28760.1"
                     /translation="MSNPALTTASVALDPTFENVWDELLWRGLVHVSTDQEALRALLA
                     GDPITYYCGFDPTAPSLHLGNLVQLLLLRRLQLAGHKPLGLVGGSTGLIGDPRPTAER
                     TLNTRETVEEWVGRLRAQVERFLSFEGDNAARMVNNLDWTAPMSAIDFLREIGKHYRV
                     GTMLKKDAVAARLNSDAGISYTEFSYQILQGMDFLELYRLYGCVLQTGGSDQWGNLTS
                     GTDLIHRVEGVSAHAIGTPLITNSDGTKFGKSEGNAIWLDAEMCSPYRMYQFWLGTTD
                     ADVVDRLKVFTFLTRAEIEEYAALVEAEPFRRAAQKRLALEVCVTVHGAEATAAVIAA
                     SEALFGQGDLSALDAATLRTALEELPNATVAAGTPVTEVLVATGLVSSVSEARRAISQ
                     GGVSLDGE"
BASE COUNT          568 a         1327 c         1335 g          531 t
ORIGIN      
        1 ggcacgctgc tgcccgaccc gggcgactag gacgtgcacg gtgcgctcga gaaggcgctg
       61 atcgccgaga tcggtcccga ggtgggcggc cgcctgcgcg ccggccgcag ccgcaacgat
      121 cagatcgcga cgctcgtgcg catgtacctg atcgatcacg cccggatgat cgcccgcgac
      181 ctgctgcgcg tcatcgatgc cctggtcgcg caggccgagg cgcacccgga tgccatcctc
      241 ccggggcgca cgcacctgca gcacgcccag ccggtgctgc tcgcacacca cctgcaggcg
      301 cacgcctggc ccctggtgcg cgaactggag cgcctggtcg actggcgtcg ccgcgccggc
      361 ttctcgccct acggcggggg agcgctggcc gggtccactc tcggtctcga cccggctctc
      421 gtggctcgcg agctggggct ggaccgtccc gcggagaact cgctggacgg caccgccgcc
      481 cgcgacgtcg tcgcggagtt cgcgttcatc gccgcgatga cgggcatcga cctgtcccgc
      541 ctgtcggagg agatcatcct ctggaacacc cgcgagttcg gcttcgtcac gctcgacgac
      601 gcgtactcca ccgggtccag catcatgccg cagaagaaga accccgacat cgccgagctc
      661 gcgcgcggca agtccggccg cctgatcggc aacctgaccg ggctgctcgc cacgctcaag
      721 ggccttccgc tcgcgtacaa ccgcgacctg caggaggaca aggagccggt cttcgactcg
      781 gtccgcaccc tcgaggtcgt gctgccggcg ttcgcgggca tgatcgccac gctgcgcttc
      841 gacacgtcgc gcatggccga gctcgcaccg cagggcttct cgctggcgac ggacgtcgcc
      901 gagtggctgg tcaagcagcg cgtgccgttc cgcgatgcgc acgagatctc cggtgcgctg
      961 gtgcgcgcct gcgaggagcg cgggatcggg ctggaggatg ccgatgacga gctgctgcgc
     1021 tcggtgtcgg agcacctgac tccggccgtc cgtgacgtgc tctcgatcga gggctcggtg
     1081 gcgtcccgca ccggcgccgg aggcaccgct cctgtgcgcg tggcggagca gcgggctgag
     1141 ctcgtggcgc gcacgcagac ggcggcgcac gcgctgggtc tctgatattc acggaacatc
     1201 aggcttctcc ttatatttct gaaatatcag tgataggctg atcgcatggc agtcgtcgtg
     1261 acagccgaca tcatcggctc ccgcaggctc ctcgacaggg gagacgcgca gaacgccctc
     1321 gactcggcga tcgcacaggt ggagcgagac ctcccggtgg cggtcgaggg aatgcgtccg
     1381 accgtcggcg acgaggagca ggcggtctat ccgacgctgg aggcggcact cgggaccatc
     1441 ctgctgctga ggctcgcact gcccgacggc gtcgagttcc gctacgggat cggcatcggc
     1501 gaggtgggga ccgtcccctc cacggcatcc gatcggggga tctccgacgg tcccggctgg
     1561 tgggcggcgc gcagcgcgat cgagcaggtg gagacgcttc agcggcgcac cgcgccgcag
     1621 gcgcggacct gggtcgtcgc tcatgagagc acggacgagg tgtcgccgga cgccgtccgc
     1681 tgggcgaacg cctatctgct gtccagggac gagctggtcg gagcgatgag cgagcgcacc
     1741 cgtcgtctcg cctacggccg ctgcctctcc cgcactcagt ccgaactcgc cgcgtccgag
     1801 ggcatcacgc agtcggccgt gtcccaggcg ctgtcgtcat cgggggcggg cagcgtcgtg
     1861 gaggggttcc ggctgctcga gctgacctga acggagcgcg ctcagacgag cgcgagacgg
     1921 atcaccgtgc cgaccgcgcc ggcccagagc agactggcga gggtgccgat gatgaagcgc
     1981 tccctggcct cggcctcggc gagctccggg aaccggccga tgcccttcac cgcgatgatc
     2041 gcggcgatgg cctcggggaa acccaccgcg atcgccacca cggtgcagat gcgctccagg
     2101 tagccgatga cggtgccgcc gcgcatgagg gtccgcgcgt gcgcggggtc ggcatcgtgc
     2161 gaggcgagga tcacgatgcc gccgtcctcg gtctcgcgaa cgcggttgcc tgcggcgatg
     2221 tcgagcgcgc gtcgggtgaa cggattgccg ccgaccgtcg cggcggcgaa ccccagcacg
     2281 gtgatcggga cgcccagcag gggcgtcgcg gctggaggca ggaccacgac gatcgtcagc
     2341 gcggatgcca cgagcgcacc gccgacggcg gccaggacgg tcgacggccg gcgcagcccc
     2401 acgagcgaga gcacgagcac caccagcagc accacgacga tcgctgaggc cagcattgcg
     2461 gaggcgaagg ggtccaggga cggcatgccg ccagcatggc acgggcctcc gacgtcacgc
     2521 gggagcggag cgcgcgatcc gcgctgatag cctggaacgc gtgtcgaatc ccgctctgac
     2581 gaccgcctcc gtggcgctcg atcccacgtt cgagaacgtg tgggacgagc tgctgtggcg
     2641 cggcctcgtc cacgtgtcca ccgatcagga ggcgctgcgc gcccttctcg ccggggaccc
     2701 gatcacgtac tactgcggct tcgacccgac ggcccccagc ctgcacctcg gcaacctcgt
     2761 gcagctgctc ctgctgcgcc gtctgcagct cgccgggcac aagcccctcg ggctggtcgg
     2821 cggctccacc ggcctgatcg gcgacccccg cccgaccgcg gagcgcacgc tgaacacgcg
     2881 cgagacggtc gaggagtggg tgggccggct gcgcgcccag gtcgagcgct tcctcagctt
     2941 cgagggcgac aacgccgcgc gcatggtcaa caacctcgac tggacggcgc cgatgagcgc
     3001 catcgacttc ctgcgcgaga tcggcaagca ctaccgcgtc ggcacgatgc tgaagaagga
     3061 cgcggtcgcc gcgcgcctga actcggacgc cgggatcagc tacacggagt tcagttacca
     3121 gatcctgcag ggcatggact tcctggagct ctaccgcctg tacggctgcg tgctgcagac
     3181 cggcggctcc gaccagtggg gcaacctgac cagcggcacc gacctgatcc accgcgtcga
     3241 gggcgtgtcc gcgcacgcga tcggaacgcc cctgatcacc aacagcgacg gcaccaagtt
     3301 cggcaagagc gagggcaacg cgatctggct ggacgccgag atgtgcagcc cgtaccggat
     3361 gtaccagttc tggctcggga cgacggatgc cgacgtcgtc gaccggctga aggtcttcac
     3421 gttcctgacc cgcgccgaga tcgaggagta cgcggccctg gtcgaggccg agccgttccg
     3481 ccgtgccgcg cagaagcgtc tcgcgctcga ggtgtgcgtg acggtgcacg gagcggaggc
     3541 gacggccgcc gtgatcgcgg catccgaggc tctgttcggc cagggggacc tgtccgcgct
     3601 cgacgcggcc acgctgcgca ccgccctcga ggagctcccg aacgcgacag tcgccgccgg
     3661 cactccggtc accgaggtgc tcgtcgccac ggggctggtc tccagcgtct ccgaggcgcg
     3721 ccgcgcgatc tcgcagggcg gggtctcgct ggacggcgag c
//
LOCUS       DATMSX010000214         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_97751,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000214 DATMSX010000000
VERSION     DATMSX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97751"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VN041_06655"
     CDS             <1..738
                     /locus_tag="VN041_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWU28761.1"
                     /translation="TVVATGLAAIAPAVTIPAVPATAEAGDGLTVTAYNLRMGYGENG
                     TFDPIGVAEQIRASGARVVLLSEVDRGWLLNGGQDQLAILSRMLGMQAVFGPAGDQIW
                     GDAILTDLPLSDVSKTRYPMFDALTGAGITAATVTWDDRPVRILATHLQPDGNETDAT
                     VRQAQLFADALGAGDGAVIGGGDLNTTRGSDAWRALLASGVQDAFADASDQPEIDHLL
                     VAGLTAENAEIIDSPLSDHPMIVATFR"
     gene            complement(816..1379)
                     /locus_tag="VN041_06660"
     CDS             complement(816..1379)
                     /locus_tag="VN041_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWU28762.1"
                     /translation="MTTIGYIVGSISSTSINRTLAEALAKLAPEGVEFREIAIKDLPF
                     YSPDLDGSFPQVALDFKQAIADVDGVLIVTPEYSRSIPGVLKNALDWSARPYGQASFD
                     GKPVAVIGASGGGIATAAAQQHLRAILGHYNAVTMGQPEGFIQLTPGLIDADGNVTVE
                     STREFLGFYLQSFLTLIARVEAARKHA"
     gene            1512..1652
                     /locus_tag="VN041_06665"
                     /pseudo
     CDS             1512..1652
                     /locus_tag="VN041_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate transporter"
BASE COUNT          253 a          561 c          605 g          251 t
ORIGIN      
        1 acggtggtgg cgaccggact cgcggcgatc gcccctgccg tcacgatccc cgcggtgccc
       61 gcgaccgccg aggcgggcga cgggctcacg gtcaccgcct acaacctgcg gatgggctac
      121 ggcgagaacg gcacgttcga cccgatcggc gtggctgagc agatccgcgc ctccggtgcg
      181 cgcgtcgtgc tgctcagcga ggtggaccgc ggctggctgc tcaacggcgg gcaggatcag
      241 ctggcgatcc tctcccggat gctcggcatg caggccgtct tcggccccgc gggcgatcag
      301 atctggggcg acgcgatcct caccgacctg ccgctgagcg acgtgtcgaa gacgcggtac
      361 ccgatgttcg acgccctgac gggagccggg atcacggccg cgaccgtgac ctgggatgac
      421 cgtccggtgc gcatcctcgc gactcacctg cagcccgatg ggaacgagac cgacgcgacc
      481 gtccgccagg cgcagctgtt cgccgacgct ctcggagccg gtgatggcgc ggtcatcggg
      541 ggcggcgacc tgaacacgac gcgcggctcg gacgcgtggc gtgcgctgct ggcatccggc
      601 gtgcaggacg cgttcgcgga cgcctcggat cagcccgaga tcgaccacct gctcgtcgcc
      661 gggctgacgg cggagaatgc cgagatcatc gactccccgc tgtccgacca cccgatgatc
      721 gtcgcgacct tccgctgagc cccgagacgc cggatgcccc cggccgtgag gcgccggggc
      781 atccgatcgt cgcgcccgcc ggcgcgcctg cgaggtcagg cgtgcttgcg agcggcctcg
      841 acgcgggcga tcagcgtgag gaacgactgc aggtagaagc cgaggaactc cctggtgctc
      901 tcgaccgtga cgttgccgtc ggcgtcgatg agacccgggg tgagctggat gaagccctcg
      961 ggctgaccca tggtcacggc gttgtagtgc ccgaggatgg cacgcaggtg ctgctgcgcg
     1021 gccgcggtgg cgataccgcc gcccgaggcg ccgatgacgg cgacgggctt gccgtcgaag
     1081 gaggcctggc cgtaggggcg ggccgaccag tcgagggcgt tcttcagcac gccggggatc
     1141 gagcggctgt actcgggggt cacgatcagg acgccgtcga cgtcggcgat ggcctgcttg
     1201 aagtcgaggg cgacctgcgg gaaggagccg tcgaggtcgg gcgagtagaa cggaaggtcc
     1261 ttgatcgcga tctcacggaa ctcgacgccc tcgggagcga gcttcgccag ggcctcggcc
     1321 agagtgcggt tgatggaggt gctggagatg ctgccgacga tgtagccgat ggtggtcatt
     1381 ggagatcctt ctcgaaagat gcaggggtac ggcggggttc aacccgtcgc acgtggactc
     1441 tattccgccg catataaaaa tcctctgaga gcagccgcta ccctcggatc atcccgcccg
     1501 atgaggagcc gatgaccgcc accacccgcc gacccctgtc gcgtcctgtc accgccggca
     1561 tcgtgaccgc cctcgtcggc ttcacgagct cgttcgccgt cgtgctcacc ggcctggacg
     1621 ccgtcggcgc gaatccggcg caggcggcga gctgctgcat caggcggtag
//
LOCUS       DATMSX010000215         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_144939,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000215 DATMSX010000000
VERSION     DATMSX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144939"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VN041_06670"
     CDS             complement(<1..909)
                     /locus_tag="VN041_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWU28763.1"
                     /translation="MTRFWVGGYGDDMDGDAEGIGFLSVDEGRGASTLTYRGTAAPVS
                     SPSWLAQHPTLDVVYAALEGSAAVQAFARSGEATLRPLGRPVDAGKYVCHLAVSPDGR
                     MLIASCYGDGRVVRYALGADGSILPAAPDKAAALRAALFGEAEPADDTDPAAAKDPYP
                     TPTGEEPRVSHAHAAVFLPDGRIATTDLGFDLVRIWRSAGTGLALDHEVVLPRGTGPR
                     HMVMHPSGHLHVVTEYSCEVFTLASARDGSWALASATTASPIAQVGFDTPAELALSRD
                     GHTLYTALRGSNTISALRVRGSGEAVE"
     gene            complement(1045..2331)
                     /locus_tag="VN041_06675"
     CDS             complement(1045..2331)
                     /locus_tag="VN041_06675"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWU28764.1"
                     /translation="MPGIVIVGVQWGDEGKGKATDLLGERTDWVVKFNGGNNAGHTVV
                     VGDEKYALHLLPSGILTPGVTPVIGNGVVVDLEVLFQELEALSARGVDVSKLKVSANA
                     HIITQYHRTLDKVTERFLGKRNIGTTGRGIGPAYADKINRVGIRVQDLFDENILRQKV
                     EGALDQKNHLLVKIFNRRAITCDEIVDELLTYADRLRPMVADTGHLVAEALDRGEVVV
                     FEGGQATMLDVDHGTYPFVTSSTATSAGAAAGSGVGPNRLDRIVGIVKAYTTRVGSGP
                     FPTELLDEMGEWLRKTGFEYGTTTGRLRRTGWYDAPITRYATRVNGITDLVLTKLDVL
                     TGLEQIPVCVAYDVDGVRFDDVPVNQTDFHHAKPIYEYFPGWSEDISKARSFEDLPQT
                     AQEYVLALEKMSNTRISVIGVGPEREQVIVRHDLVD"
     gene            2508..3449
                     /locus_tag="VN041_06680"
     CDS             2508..3449
                     /locus_tag="VN041_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU28765.1"
                     /translation="MRSSPDPALDETAPPAKAARPNGPLVLLAALVTVVLWASAFIGI
                     RGAGPHYDPGALALLRMAVGTLALGVIALRRGIRVPARRQLPLVVVWGVGWFCVYNLA
                     LNAAERTLDAGTAAMVVNLAPLMVVVFSGLFLREGFPKPLIIGAPLAFLGVVLIGMTS
                     HTDAGLDVTGLLLALLAAVMYAGCTLLQKHLLSAGADSTTLTFFGAAAGTIALLPWTG
                     GVIQAVQTAPLDATLWVVYLGIFPTAVAFTTWAYVLQRSTAGQTSATTYVVPAIAILL
                     SWLILGEVPTPLMLLGGALCLLGVLVTRMRWRRRVRE"
     gene            3513..4253
                     /locus_tag="VN041_06685"
     CDS             3513..4253
                     /locus_tag="VN041_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HWU28766.1"
                     /translation="MRTAGAVTTWWPWARIAASALGFAAIIGQLALTLSIAAHAKTEW
                     GGHLPTVAANFFSFFTILSNLGAAVILAIAAIWAWTKGRDAAQEPRWLAVTLACVATY
                     MIVTGIVYNVLLRGIALPQGSTLGWSNEVLHVVIPLFLLVDVLFAPKRRMLSWGTIGA
                     IAAFPIVWAIYTLVRANLIIAPATGDPYWYPYPFLNPHITPGGYLGVAGYIVGIAVAI
                     IAVGAGVVWVARKRAASVAESAAEPATV"
     gene            complement(4263..5285)
                     /locus_tag="VN041_06690"
     CDS             complement(4263..5285)
                     /locus_tag="VN041_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HWU28767.1"
                     /translation="MPGNRLDERELTAPVVLTRADGTLNADAVGWARSPLVDTSGIAR
                     DGLRFAGRNKRWEYWGITTPTHVIGMTVSSIDYAAVHEVWVLDRATERTWHRSVTAIP
                     ARGVTLPPSAEQGPSRARAKDLVIDIDELPEGTRLRARIPGAAIDVIARLPEDHERLA
                     VVVPWSRTRFQYTLKDVARPASGTLTIDGAVHVLPEGESWAVLDHGRGRWPYDVTWNW
                     GAGSGRSGGRVIGIQVGAKWTDGTGSTENAFLVDGRLHKISVELDWRYDIADWRRPWR
                     ISGGGLDAVFTPAYDKQTRTNLGILSSSTDQCFGDWSGTFRTDDGEAIAFEGIFGWAE
                     EVHNRW"
     gene            5365..>5723
                     /locus_tag="VN041_06695"
     CDS             5365..>5723
                     /locus_tag="VN041_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28768.1"
                     /translation="MTDDWSEATAELWARAAHPLRRDARAAAPAGLPAATPIAHAPQQ
                     GAVVHRGLLIAGFAFIAVIGLAATTVGILTSGVGAGVTADASAGRQGTTSLLAERGVP
                     SVADDEPEESADAGDAGA"
BASE COUNT          771 a         1990 c         2054 g          908 t
ORIGIN      
        1 ctcgaccgcc tcgccggaac cgcgcacccg cagcgccgag atcgtgttgc tgccgcgcag
       61 cgccgtgtac agcgtgtgcc cgtcgcggga gagggcgagc tcggcggggg tgtcgaatcc
      121 gacctgtgcg atcggcgagg cggtggtcgc cgatgccagc gcccagctgc cgtcccgggc
      181 ggatgccagg gtgaacacct cgcaggagta ctccgtcacg acgtgcaggt gaccgctggg
      241 gtgcatgacc atgtgccgcg ggccggtgcc gcggggcagc acgacctcgt gatcgagagc
      301 cagtccagtg ccggcggacc gccagatgcg caccaggtcg aagccgaggt cggtggtggc
      361 gatgcggccg tcgggcagga acacggcggc atgcgcgtgc gagacacgcg gctcctcgcc
      421 ggtcggggtg gggtacgggt ccttcgccgc ggcggggtcc gtgtcgtccg cgggttccgc
      481 ttccccgaaa agcgccgcgc gcagtgccgc ggccttgtcg ggtgcggcag gcaggatgct
      541 gccatcagcg ccgagcgcgt agcggacgac gcgtccgtcg ccgtagcagc tggcgatcag
      601 catccgcccg tccggggaga ccgcgaggtg gcagacgtac ttgccggcgt cgacagggcg
      661 gccgagcgga cgcagtgtcg cttcgccgct gcgagcgaag gcctggacgg cggccgagcc
      721 ctccagcgcc gcgtacacga cgtcgagggt gggatgctgc gccagccacg acggggacga
      781 gaccggcgcc gccgtgccgc ggtaggtgag cgtcgacgcg ccacgcccct cgtcgacgga
      841 gaggaagccg atgccctcgg catccccgtc catgtcgtcg ccgtatccac cgacccagaa
      901 ccgcgtcatg cccactcctc cgatgtcgaa ctgcttctcg agtgcaccgc ttcgcgacga
      961 gggaaccacc tgccgacgcg gagaaccggt tcactcgacg ggaaccggtg caccggcgcc
     1021 ggaaacgcgc tccggcggcg tgcgtcagtc gacgaggtcg tgacgcacga tgacctgctc
     1081 gcgctcggga cccacgccga tcaccgagat gcgcgtgttg ctcatcttct ccagcgccag
     1141 cacgtactcc tgggcggtct gcggcaggtc ctcgaaggag cgcgccttcg agatgtcctc
     1201 gctccagccc gggaagtact cgtagatcgg cttggcgtgg tggaagtcgg tctggttgac
     1261 cggcacgtcg tcgaagcgca cgccgtcgac gtcgtaggcg acgcacacgg ggatctgctc
     1321 caggccggtc agcacatcga gcttggtgag caccaggtcg gtgatgccgt tcacgcgcgt
     1381 ggcgtagcgg gtgatcggtg cgtcgtacca gccggtgcga cgcagacggc ccgtcgtggt
     1441 gccgtactcg aagccggtct tgcgcagcca ctcgcccatc tcgtcgagca gctcggtggg
     1501 gaacggaccc gagccgacgc gggtcgtgta cgccttcacg atgccgacga tgcggtccag
     1561 gcggttcggg cccacaccgg agccggcggc cgcacccgcg gaggtcgcgg tggacgaggt
     1621 cacgaacggg taggtgccgt ggtcgacgtc gagcatggtg gcctgaccgc cctcgaacac
     1681 gacgacctcg ccgcggtcga gcgcctcggc gaccaggtga ccggtgtcgg cgaccatcgg
     1741 gcgcagccgg tctgcgtagg tgagcagctc gtcgacgatc tcgtcgcacg tgatcgctcg
     1801 gcggttgaag atcttcacca gcaggtggtt cttctggtcg agcgcgccct ccaccttctg
     1861 ccgcaggatg ttctcgtcga acaggtcctg cacgcggatg ccgacgcggt tgatcttgtc
     1921 ggcgtaggcg gggccgatgc cgcggccggt ggtgccgatg ttgcgcttgc cgaggaagcg
     1981 ctcggtgacc ttgtcgagcg tgcggtggta ctgcgtgatg atgtgcgcgt tcgcgctgac
     2041 cttgagcttg gagacgtcga cgccccgggc ggagagcgcc tcgagctcct ggaacagcac
     2101 ctccaggtcg acgaccacgc cgttgccgat caccggcgtc acgccggggg tcaggatgcc
     2161 ggagggcagc aggtgcaggg cgtacttctc gtcgccgacg acgacggtgt gcccggcgtt
     2221 gttgccgccg ttgaacttga cgacccagtc ggtgcgctcg ccgagcaggt ctgtcgcctt
     2281 gcccttgccc tcatcgcccc actggacgcc gacgataacg attcctggca tgggtgtacc
     2341 ccctggtact gcacgctgtt cgccgggttt gcaccggcag atgagctggc ccgaacgggc
     2401 gtgtccatcc taccggaggg cctgcgacag ccccttggcg ggaatcggat gccggatggc
     2461 tgccgacgcg gtcgaccgat gtcggcggca cctgggacga tcggaggatg cgatcatcac
     2521 cggaccccgc gctcgacgag accgcacctc ccgcgaaggc cgcgcgcccg aacgggccgc
     2581 tcgtcctgct cgctgcgctg gtcacggtcg tgctgtgggc gtcggcgttc atcgggatcc
     2641 gcggcgcggg tccgcactac gaccccggcg cgctcgccct gctgcggatg gccgtcggca
     2701 cgctcgcgct cggcgtgatc gcgctgcgac gcggcatccg cgtccccgcc cgcaggcagc
     2761 ttccgctcgt ggtggtctgg ggcgtcggct ggttctgcgt gtacaacctg gcgctgaacg
     2821 ccgccgagcg cacgctcgac gcgggcaccg cggcgatggt cgtgaacctc gctccgctca
     2881 tggtggtggt gttcagcgga ctgttcctgc gagagggctt cccgaagccg ctgatcatcg
     2941 gcgcaccgct cgcattcctg ggtgtggtgc tgatcggcat gacctcgcac acggatgccg
     3001 ggctcgacgt caccggactg ctgctggccc tgctcgccgc cgtcatgtac gccggatgca
     3061 ccctgctgca gaagcatctg ctcagcgcgg gggcggactc caccacgctc accttcttcg
     3121 gtgcggccgc gggcacgatc gcgctgctgc cgtggaccgg gggcgtgatc caggccgtgc
     3181 agaccgcgcc gctcgacgcc accctgtggg tcgtctacct cggcatcttc cccaccgcgg
     3241 tcgccttcac cacctgggcg tatgtgctgc agcgcagcac cgccgggcag acctccgcca
     3301 ccacgtacgt cgtccccgcg atcgcgatcc tgctctcctg gctgatcctc ggcgaggtgc
     3361 cgacgccgct catgctgctc ggcggcgcgc tgtgcctgct cggcgtgctc gtgacgcgca
     3421 tgaggtggcg gcggcgcgtg cgcgaatgac cgcgtcgttc cgctccccgt cgctacagtg
     3481 agcgtcgggg gaccgagagc gtcggaggaa caatgcgcac cgcaggagca gtgacgacct
     3541 ggtggccgtg ggcccgcatc gcagcatccg ccctgggctt cgccgcaatc atcggacagc
     3601 tcgcgctgac gctgagcatc gccgcgcacg cgaagaccga gtggggcggg cacctgccga
     3661 ccgtcgcggc gaacttcttc agcttcttca cgatcctgtc gaacctgggc gctgcggtca
     3721 tcctcgcgat cgccgcgatc tgggcctgga cgaagggccg ggacgccgcg caggagcccc
     3781 gctggctggc cgtgaccctc gcctgcgtgg cgacgtacat gatcgtcacc ggcatcgtct
     3841 acaacgtgct gctgcggggc atcgcgcttc cgcagggctc gaccctgggc tggtcgaacg
     3901 aggtgctgca cgtggtcatc ccgctgttcc tgctggtcga cgtgctgttc gccccgaagc
     3961 gccggatgct gtcctggggc acgatcggcg ccatcgccgc gttcccgatc gtctgggcga
     4021 tctacacgct ggtgcgcgcg aacctgatca tcgcgcccgc caccggcgat ccctactggt
     4081 acccctaccc gttcctgaac ccgcacatca ccccgggcgg ctacctgggc gtggcggggt
     4141 acatcgtcgg gatcgcggtc gcgatcatcg cggtgggtgc gggcgtggtg tgggtcgcgc
     4201 ggaagcgcgc ggcatccgtg gccgagtccg cggcggagcc ggccacggtc tgaggcgccc
     4261 gctcaccagc ggttgtgcac ctcctcggcc cagccgaaga tcccctcgaa cgcgatggcc
     4321 tcgccgtcgt cggtgcggaa cgtgcccgac cagtcgccga agcactgatc ggtggaggag
     4381 gagaggatgc cgaggttcgt gcgcgtctgc ttgtcgtagg cgggcgtgaa cacggcatcc
     4441 agtccaccgc cgctgatccg ccagggccgg cgccagtcgg cgatgtcgta ccgccagtcg
     4501 agctccacgc tgatcttgtg caggcgtccg tcgacgagga acgcgttctc cgtcgagccg
     4561 gtgccgtcgg tccatttcgc gccgacctgg atgccgatca cgcggccacc ggacctgccg
     4621 gagccggcgc cccagttcca ggtgacgtcg tagggccagc ggccccgccc gtggtcgagc
     4681 acggcccagg actcgccctc ggggagcacg tgcacagcgc cgtcgatggt gagggttccg
     4741 gatgccggcc gggcgacgtc cttgagcgtg tactggaacc gggtgcggct ccacggcacc
     4801 accacggcca gtcgctcgtg gtcttcgggc agccgcgcga tgacgtcgat cgcggcgccg
     4861 gggatgcggg cgcgcagccg ggtgccctcc ggaagctcgt cgatgtcgat cacgaggtcc
     4921 ttcgcgcggg cgcggctcgg cccctgctcg gcgctcggcg gaagggtcac tccgcgcgcc
     4981 gggatggcgg tgacggagcg gtgccaggtg cgctcggtgg cccgatcgag cacccacacc
     5041 tcgtgcaccg ccgcatagtc gatggacgac acggtcatgc cgatcacatg cgtcggagtg
     5101 gtgatccccc agtactccca gcgcttgttg cggcccgcga agcggaggcc atcccgcgcg
     5161 atccccgagg tgtccacgag cggcgagcgc gcccagccca cggcatccgc attcagcgtc
     5221 ccgtcggcac gggtgagcac gacgggtgcg gtgagttcgc gctcatcgag acggttcccg
     5281 ggcacggcgc ccccctcctg tcctgtgatc ccaggatgcc agcaggcata ctggaggtcg
     5341 accggagtgg tcagggggtg tgtgatgacg gacgactgga gtgaggcgac ggccgagctg
     5401 tgggcccgcg ccgcccatcc tctgcgccgc gatgcgcgtg ccgccgcacc agccggtctc
     5461 cccgcagcga ccccgatcgc gcacgcacct cagcagggcg ccgtcgtgca ccgcggcctg
     5521 ctgatcgccg gtttcgcgtt catcgcggtg atcggcctgg cggccacgac cgtcggcatc
     5581 ctgacctccg gtgtcggggc gggtgtcacc gcggatgctt cggcaggtcg gcagggcacg
     5641 acgtccctgc tcgccgagcg aggcgtcccc tccgtggcgg acgacgagcc cgaggagtcg
     5701 gcggatgccg gggacgcggg cgc
//
LOCUS       DATMSX010000216        14534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3329,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000216 DATMSX010000000
VERSION     DATMSX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 14534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14534
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3329"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VN041_06700"
     CDS             <1..358
                     /locus_tag="VN041_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWU28769.1"
                     /translation="LRRTDGDLDRLTGILQRRRRAAEEGDEAAFVQADVDFHVGLADA
                     SHNRTLREMYRSFSAVLGSTVELSDCYGPGSIELSRDHEDLLDVVRAQDHSAVVRAAL
                     RILDAQESAVRDRERD"
     gene            548..1639
                     /gene="ald"
                     /locus_tag="VN041_06705"
     CDS             548..1639
                     /gene="ald"
                     /locus_tag="VN041_06705"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWU28770.1"
                     /translation="MRIGVPTEIKNNENRVALTPAGADRLVHEGHRVLVQSGAGLGSR
                     ITDEAYLAAGAEIVSSAEEAWGEADLLLKVKEPIAQEYGFLRPELTLFTYLHLAADRA
                     LTGALVDAGTTAVAYETVQLPDRSLPLLVPMSEIAGRLSVTVGSYSLMRSNGGRGTLL
                     GGIAGTPRAKTVVIGGGVAGEHAAANALGLGSKVTVFDISLPRLRELEHRYGGALETR
                     ASSRYDIAEELATADLVIGSVLIPGAAAPKLVTDEMVAGMKPGSVLVDIAIDQGGCFE
                     GSHPTTHDDPTFAVHDSIYYCVANMPGAVPETATRALTNATLPYVSAIAGKGWDRAAS
                     DDAALAKGLNVKDGRITLPAVAEAHGLAA"
     gene            1701..2918
                     /locus_tag="VN041_06710"
     CDS             1701..2918
                     /locus_tag="VN041_06710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWU28771.1"
                     /translation="MTERAPLSRKLSAIAESATLKVDAKAKALKAEGKPVISYAAGEP
                     DFATPQFIVDAAAEALADPANYRYTPAPGLPALREAIAAKTLRDSGLEVSPSQVIVTN
                     GGKQSVYQAFQTVVNPGDEVLLPAPYWTTYPEAIRLADGTPVEVFAGADQDYKVTVAQ
                     LEAARTERTTVLVFVSPSNPTGSVYSADETKAIAEWALEHGIWIISDEIYQNLTYDGV
                     KAASVVQALPEIANQTILVNGVAKTYAMTGWRVGWMVGPADAIKIAGNLQSHLSSNVN
                     NVAQRGAIAALNGPQDEAEQMRQAFDRRRRLIVSELSKIEGLVVPTPTGAFYVYPDVQ
                     GLLNRTWTRRDGSTVTPTTSLELADFILDEAEVAVVPGEAFGPSGYIRMSYALGDDQL
                     LEGVQRLQKLFAS"
     gene            complement(3012..3410)
                     /locus_tag="VN041_06715"
     CDS             complement(3012..3410)
                     /locus_tag="VN041_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU28772.1"
                     /translation="MSGLIPYLLFPGDAAEALQQYAAIFGGELQMFTYAQAGRTDGPE
                     DAIAHGMLVNGPVDLAGADAATGEDTVAMSGMFLSLLGTADPGTLAGWFDALAAGGEV
                     IDPLQKRPWGDSDGTLVDRHGVRWLIGHQG"
     gene            complement(3449..4591)
                     /locus_tag="VN041_06720"
     CDS             complement(3449..4591)
                     /locus_tag="VN041_06720"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWU28773.1"
                     /translation="MASREVTPIPLRQLTTLRTGAAPERMLDAGSTAELVDALHEVWA
                     TGDEWFVLGGGSNLFVGDEPFEGAVIRILTSGIEELPSPHEGRIRLRAQAGHGWDDLV
                     AYAVARGYAGIEAMSGIPGTVGAAPVQNVGAYGQEIQETLVEVELIDEGASAPVVVPA
                     AELGLGFRTSVLKHHYGGVPQRRAVITSVTLDLAVTGDAGRVIRGEQLRRALSLEGFD
                     PVPLEWVRTRILATRTAKGMLLNEDDPDTHSAGSFFQNAIVPEHVARTLPPECPRWPV
                     EPDLDTMTVIPLENYYGLTPRADRPEGLVKVSSAWLIEHSGIGKAFKLPRSRASVSTK
                     HALALTNRGGANAAELSELARFIQSRVHSEFGLLLQPEPVLVGVEL"
     gene            complement(4593..4994)
                     /locus_tag="VN041_06725"
     CDS             complement(4593..4994)
                     /locus_tag="VN041_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWU28774.1"
                     /translation="MAGFTVGDVIAERTVHLTRESLVRYAGASGDFNPIHYRDDVAAA
                     VGLPGVLAHGMLTMGLASSVVVAALPADARLIDYGVRFTKPVVVDPEDGADVHVLAKV
                     GAVDETSARIDLTVKAAETTVLVKAQLRVAL"
     gene            complement(4994..5455)
                     /locus_tag="VN041_06730"
     CDS             complement(4994..5455)
                     /locus_tag="VN041_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWU28775.1"
                     /translation="MPVNNDLVGREFPPTAPYLVGREKVREFARAVFADAPQHADVAA
                     AQAAGYADVVAPPTFAMVIQDLTLQQLLAEPDSGIVLARTIHAEQRFTYSRPIVAGDE
                     LTAQLSVTGIRALGGNAMITSDATITDAAGEHVVTATSVLLVGSDSEGEDA"
     gene            5618..9421
                     /locus_tag="VN041_06735"
     CDS             5618..9421
                     /locus_tag="VN041_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HWU28776.1"
                     /translation="MKLNSPSCRPRLRALGLAIALGVSGIGIAGTASAASAASVAQTV
                     AKASSAPTPITPRPAAPEVPVDSTGTSIGSITDVQRDGASVTLTAEHGAMRVTFLEDG
                     TFRLEADPSGSFTDPANTPQGDAARTADIVVGAKTFGGADVAIAEGDVIRIASKAVTV
                     SVDRATGAVSATRADGSTIFAESAAITFGKSSATQHLKGQKGEQILGGGMQNGRSIHT
                     GSVINIANNFDWDDDGYPNAVPYYMSSEGYGVLRNTFAKGTYDFAQHTTTHQERRFDA
                     YYFVGDYEQSLESYTQLTGRPMMPPVYALEYGDADCYNRSSPTYTGTRTPSKLRTPQA
                     LDVAKGFVENDMPAGWMLVNDGYGCEYQELPETVDAIQEQTDLKTGLWTQRSLDKQEY
                     EVGEAGVRLRKLDVAWVGSGYRLALTGCEAAHDGIEKYSDARGTALMVEGWAGSQRCG
                     MQWTGDHSGNLDAVRWQISALTGAGNSGLAFSTGDVDGIFGGSPESYVRDLQWKAFAP
                     ALYSMSGWASVDKRPWLYGDEATKINRSYLQLRQRLMPYIYTLAAESHRSGLPMMRSL
                     ALEHPKDPGSYSVEANNEFLLGSDYLVAPVFTKTDVRNGIYLPAGSQWVDFWTGAVHE
                     GGQVLNGHPAPLDRLPIFVRAGAVVPQGITARNASLVPANSPVTLSVFPQGESSFSLY
                     EDDQVTRAYADGASSTQKFTVQAPGAGKKGSVTIGIGERAGSYDGKADARPYVIEAHT
                     GSAPANVSIDGKGLKQAASREALDAASSGWFFDAEDAGGVIRVKVPAVSSSASAEVVL
                     AATSAVGGRDADLTSASVSVTLDDRVFQGDTTSVEATFRNTGTKKKENVTLTPQLPEG
                     WSLVSAKGDRTSVVEPGASVTATFEAKVTDAAAADLQTVRVVADYTTQKTAQQASGAN
                     QIYVAYGSLAGAYNADAVTTIDTAKAGDFDGGGATFSAEALARAGVTPGSTVPVGTGD
                     AAIAYTWPSPSGAKDSVSPAGQTIALTGKGTHLAVLASAASGGGVNPALELHYADGTI
                     SKQNVFFPNWLPQASGLGGASVAITSLGRNSATNPNVYEYPTGRYQVYSNLVRLNPAK
                     ELAYVVLPTESRLKIFDWKVVDQPMPPAPSGTVYASDLNWISATNGWGVIGRDVANKD
                     SATSPDLPLAINYIDPATGSSPTYEKGLGVHATSKVTYYLGGKCSSFTASVGLEKDFA
                     GNVIFTVDTDGTNRYQSRTFTPGFAPEEVNVDLTGVQYIDLNVTAPGSINGAHGVWGD
                     ALFHC"
     gene            complement(9520..10308)
                     /locus_tag="VN041_06740"
     CDS             complement(9520..10308)
                     /locus_tag="VN041_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWU28777.1"
                     /translation="MRDEQANTRGPRAYAGFVAIGLIAGLLSGLFGVGGGTVIVPLLV
                     LLLGFDQRLAAGTSLTAIVPTASVGVISYALSGSVAWIPALILAAGAVAGAQIGSRLM
                     PRISQRALRWGFVAFLLVVLVSLFLVIPSRDSVFHLTWLNGAGLVLVGILTGVIAGLI
                     GVGGGVVVVPVLMVVFGTSDLVAKGTSLLMMIPTAVSGTIGNLRHRNVDMAGALIIGI
                     SACTTTALGAWLATLVDPATGNLLFAAYLVAIAVQMSIKAIRSR"
     gene            complement(10361..11908)
                     /locus_tag="VN041_06745"
     CDS             complement(10361..11908)
                     /locus_tag="VN041_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWU28778.1"
                     /translation="MDSWSLLLEGFATALQPEYLMWAFIGVFIGTAVGVLPGIGPAMT
                     VALLLPLTYTLDATAAIITFAGIYYGGMYGGSTTSILLNTPGESASIVTAIEGNKMAK
                     LGRGAAALATAAIGSFIAGTIATVGLTLLAPILADFAVSLGNPDKLALIVVAFVTVGA
                     LMGKSVPRGVLSLAIGLFIGLVGTDALSGQQRYTLGIPELNQGISVVLVAVGLFAVGE
                     TLYVASRMRHGGVDVIPVTKGWSNWMTKEDWRRSWKPWLRGTAIGFPIGTIPAGGADV
                     ATFLSYAAEQKLSKHKEQFGRGAIEGVAGPEAANNAAAAGVLVPLLTLGLPTTATAAI
                     ILFAFQSYGIQPGPLLFANQPALVWALVASLYLGNVILIVLNLPLVGMWVKLLQIPRP
                     YLYAGILMFAAFGAYAIKFNITDIIILLIIGVLGYFMRRYGIPVAPLVVGMILGPMGE
                     GELRKALQLSQGDPMTLIASPFAAIAYGVLVVLILGALWLRRRQTRYEQHLTESIAVP
                     IKADQEV"
     gene            complement(11908..12453)
                     /locus_tag="VN041_06750"
     CDS             complement(11908..12453)
                     /locus_tag="VN041_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HWU28779.1"
                     /translation="MTAPITGANGRAEETRPAPRVPIGELVFALLMLALGVLALVGAF
                     SIHVPVGIQVGPTVFPIAVSVLLIGSAAAVLIGVLRGERAEVEEGEDIDPDASTDWVT
                     LAKIVGAIVVHLLLIELIGWAPAAAVLFGIVAWALGAKRWWLGFVIGLVVALVVQIVF
                     GGFLGLSLPWGPAFGWLGGMF"
     gene            complement(12455..13489)
                     /locus_tag="VN041_06755"
     CDS             complement(12455..13489)
                     /locus_tag="VN041_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWU28780.1"
                     /translation="MMNQRLKKSRNAIVAIAAIAALALAGCSAQNGGGDKTDAGGKDA
                     KSSFSNVSIVVPADPGGGWDQTGRTMAKVLTEDKIVKTAPVTNVGGAGGTIGLAQLAT
                     EKDPATLMVNGLVMVGAIETNASKVRLEDTTPIARLTDEPLVVVVPADSKYKDLKSLV
                     EDIVDKGQAITVTGGSAGGADHILAGLLLDAAGVKSADIPAKLNYTPNAGGGEAVSLI
                     LGGKVAAGISGVGEFQQHIEAGKMRALAVSSEKPVEQLPDVPTITDAGYDVVLTNWRG
                     VIAPGDISDADRAELERIVTEMHDSEAWKKELETKGWADAFLTGSELDSFVKQNISDV
                     QGTLKNIGLI"
     gene            13648..>14534
                     /locus_tag="VN041_06760"
     CDS             13648..>14534
                     /locus_tag="VN041_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWU28781.1"
                     /translation="MASRMKHASKKMRERRMTLRVQLILLQALIVAVVTLIAGVTAGI
                     IQAHTVRDAYEDRMLAVAQSIASMPLIRAAFDDEDPSATIQPLAEAIRRASDLTYVVV
                     ANADGIRYSHPNEALIGQKVSTDPSVPLSGKIYMGTQTGTLGPSWRVKVPIFSDDSQV
                     IGTVSVGILESQGDGEFAVYLFWIIGAMLGAVILGVFGSAWVTAVIRRRIHLLEPHQI
                     TALVRNQETTLHGLSEGVITVDAHGIVTLANDAAARLLGTDAETLTGSPAADVLGDDL
                     MAVLHDGEDAGRPVIVGSSV"
BASE COUNT         2319 a         5170 c         4912 g         2133 t
ORIGIN      
        1 cctccgccgc acggacggcg acctcgaccg gctcaccggc atcctgcagc gccgccgtcg
       61 cgctgccgaa gagggcgacg aggccgcctt cgtgcaggcc gacgtcgact tccacgtcgg
      121 gctggcggat gcttcgcaca atcgcacgct gcgcgagatg taccgcagct tctcggctgt
      181 gctcggttcg accgtcgagc tgagcgactg ctacggcccc ggcagcatcg agctgtcgcg
      241 cgaccatgag gatctgctgg acgtcgtgcg cgcgcaggac cactccgccg tggtgcgggc
      301 ggcactgcgc atcctggatg cccaggaatc cgcagtccgc gaccgcgagc gcgactgaca
      361 cgtccgcccc tcccgagaag agccgggcgc tgatctgcgc gacccgactg ctccagatgc
      421 cgctttcgac gaagaatctg tcttctttgc ccctgatccg gaatattctt cggatcctcc
      481 cccgcagacc cgatgtttct gcgattctct tctacgctct gctgtcatcc acgcaccagg
      541 agaatcaatg aggatcggcg tacccaccga aatcaagaac aacgagaatc gcgtcgcgct
      601 cacccccgcc ggcgccgatc ggctcgttca cgagggccac cgcgtcctcg tgcagtccgg
      661 cgcaggactc ggatcgcgca tcaccgacga ggcctatctg gccgccggcg cggagatcgt
      721 ctcctcggcc gaggaggcgt ggggcgaggc ggacctgctg ctgaaggtca aggagccgat
      781 cgctcaggag tacgggttcc tgcgtcccga gctgacgctc ttcacctacc tgcatctcgc
      841 cgccgaccgg gctctcaccg gcgcactcgt cgatgcgggc accacggccg tcgcctacga
      901 gaccgtgcag ctgccggatc gcagcctgcc gctgctcgtg ccgatgagcg agatcgccgg
      961 tcgcctctcg gtgaccgtcg gctcgtactc gctgatgcgc tccaacggcg gccgcggaac
     1021 cctgctcggc ggcatcgccg gaaccccacg cgccaagacc gtggtgatcg gcggcggggt
     1081 cgcgggcgag cacgccgccg cgaacgccct gggcctgggc tcgaaggtca ccgtgttcga
     1141 catctcgctc ccccgtctgc gcgagctcga gcaccgctac ggtggcgcgc tcgagacccg
     1201 ggcctccagc cgctacgaca tcgccgagga actcgccacg gccgatctgg tgatcggatc
     1261 ggtgctgatc cccggcgccg cagcgcccaa gctcgtcacc gacgagatgg tggcgggaat
     1321 gaagccgggc tcggtgctgg tcgacatcgc gatcgatcag ggcggatgct tcgagggctc
     1381 ccaccccacc acgcacgacg accccacctt cgccgtgcac gactcgatct actactgcgt
     1441 ggcgaacatg ccgggcgccg tgcccgagac cgccacccgg gcgctcacca acgccacgct
     1501 gccctacgtg tcggcgatcg cgggcaaggg ctgggaccgc gcggcatccg acgacgccgc
     1561 cctcgcgaag ggcctcaatg tcaaggatgg ccggatcacc ctcccggcag tcgccgaggc
     1621 gcacggtctc gccgcctgat cgtccggggc gcgggtcagt attccgttct gctgatccgc
     1681 gcccgcgtac gatggatgcc gtgaccgaac gcgcaccact ctcccgcaag ctgtccgcca
     1741 tcgccgagtc cgccaccctg aaggtggatg ccaaggccaa ggccctcaag gctgagggca
     1801 agcccgtcat ctcctacgcg gccggcgagc ccgacttcgc aaccccgcag ttcatcgtgg
     1861 acgctgcggc cgaggccctc gccgacccgg cgaactaccg ctacaccccc gcccccggcc
     1921 ttcctgcgct gcgcgaggcg atcgccgcga agacgctgcg cgactcgggg ctggaggtct
     1981 cccccagcca ggtcatcgtc acgaacggcg gcaagcagtc ggtctaccag gccttccaga
     2041 ccgtggtgaa cccgggcgat gaggtgctgc tgcccgcccc gtactggacc acctaccccg
     2101 aggccatccg cctggccgac ggcaccccgg tcgaggtctt cgccggcgcc gatcaggact
     2161 acaaggtcac cgtcgctcag ctcgaggccg cacgcaccga gcgcaccacc gtgctcgtgt
     2221 tcgtctcgcc gtccaacccg accggctcgg tctactccgc ggacgagacg aaggccatcg
     2281 ccgagtgggc gctcgagcac gggatctgga tcatctccga cgagatctac cagaacctca
     2341 cctacgacgg cgtgaaggcc gcctcggtcg tgcaggcgct gcccgagatc gcgaaccaga
     2401 cgatcctcgt caacggcgtg gccaagacct acgcgatgac gggctggcgc gtgggctgga
     2461 tggtcggccc cgcggatgcc atcaagatcg ccggcaacct gcagtcgcac ctgtccagca
     2521 acgtgaacaa cgtcgcgcag cgcggcgcga tcgccgccct gaacggcccc caggacgagg
     2581 ccgagcagat gcgccaggcc ttcgaccgcc gtcgccgcct gatcgtctcg gagctgtcga
     2641 agatcgaggg cctcgtggtc ccgaccccca ccggcgcctt ctacgtgtac cccgacgtgc
     2701 aggggctgct gaaccgcacc tggacccgcc gcgacggcag caccgtgacg cccaccacct
     2761 cgctcgagct cgcggacttc atcctcgacg aggccgaggt cgcggtcgtg cccggcgagg
     2821 cattcggccc ctccggctac atccgcatgt cgtacgcgct gggcgacgac cagctgctgg
     2881 agggcgtgca gcgcctgcag aagctgttcg cctcctgacg cgctcttcgc cgacacccgc
     2941 tttccgtcga cgcccccagg cacggactcc cgtgcgtggg ggcgtcgtcg tcggaacggg
     3001 tgtgcgtgtc gtcagccctg gtggccgatc agccagcgca cgccgtgccg gtcgaccagc
     3061 gtgccgtcgg aatcacccca cgggcgcttc tgcagcgggt cgatgacctc cccgcccgcg
     3121 gcgagagcgt cgaaccaccc cgcgagcgtg cccgggtcgg cggtgccgag cagcgagagg
     3181 aacatgccgc tcatcgccac cgtgtcctcg ccggtcgcgg catccgctcc cgccaggtcg
     3241 accgggccgt tcaccagcat cccgtgcgcg atcgcgtcct cggggccgtc ggtgcgcccg
     3301 gcctgggcgt aggtgaacat ctgcagctcg ccgccgaaga tcgcagcgta ctgctgcagc
     3361 gcctcggccg cgtctccggg gaacagcagg taggggatca atccgctcat cccgcgagcc
     3421 tacgcaggaa gggtcgcgat caccagacct acagctccac ccccacgagc accggctccg
     3481 gctgcagcag caggccgaac tcggagtgca cacggctctg gatgaagcgg gccagctcgc
     3541 tgagctccgc cgcattcgcc ccgccccgat ttgtcagcgc gagggcgtgc ttggtcgaga
     3601 cggacgcacg tgaccggggc agcttgaacg ccttgccgat tccggagtgc tcgatcagcc
     3661 aggcggagct gaccttgacg agcccctccg ggcggtcggc gcgcggcgtg agcccgtagt
     3721 agttctccag cgggatcacg gtcatcgtgt cgaggtcggg ctcgaccggc cagcgcgggc
     3781 actccggcgg cagggtgcgg gcgacgtgct cgggcacgat cgcgttctgg aagaacgacc
     3841 cggcgctgtg cgtgtcgggg tcgtcctcgt tcagcagcat ccccttcgcc gtgcgtgtgg
     3901 cgaggatgcg ggtgcgcacc cactccagtg gcaccgggtc gaatccttcc aggctcaggg
     3961 cccggcgcag ctgctcgccg cggatgacgc gtccggcatc gcccgtcacg gcgaggtcga
     4021 gcgtgacgga cgtgatcacg gctcgccgct gcgggacgcc gccgtagtga tgcttgagca
     4081 ccgaggtgcg gaagcccagt cccagttcgg cggccggcac gacgaccgga gcgctcgcac
     4141 cctcgtcgat cagctccacc tcgacgaggg tctcctggat ctcctggccg taggcgccga
     4201 cgttctgcac gggtgcggcc ccgacggtgc cggggatgcc gctcatcgcc tcgatgcccg
     4261 catagccgcg cgccacggcg taggcgacca ggtcgtccca gccgtgcccg gcctgcgcac
     4321 ggagccggat gcgaccctcg tgcggagacg ggagctcctc gatgccggag gtcaggatgc
     4381 ggatcaccgc gccctcgaag ggctcgtcgc ccacgaacag gttcgagccg ccgcccagca
     4441 cgaaccactc gtccccggtc gcccacacct cgtgcagggc gtcgaccagt tcggcggtgg
     4501 agccggcatc cagcatccgc tccggagcgg cgccggtgcg cagggtggtc agctgccgca
     4561 gcgggatcgg ggtgacctct cgcgacgcca tgtcagagcg cgacccgcag ctgcgccttg
     4621 acgagcacgg tcgtctcggc cgccttgacc gtcaggtcga tgcgcgccga ggtctcgtca
     4681 acggcgccca ccttcgcgag cacgtgcacg tcggcgccgt cctccgggtc gacgacgacg
     4741 ggcttcgtga agcgcacacc gtagtcgatc agccgcgcgt cggcgggaag agcggcgacg
     4801 acgacggagg atgccaggcc catggtcagc atcccgtgcg ccaggacacc cggcaggccg
     4861 actgccgccg cgacgtcgtc acggtagtgg atcgggttga agtcgccgga tgccccggcg
     4921 tagcgcacca gcgactcgcg cgtcaggtgc acggtgcgct cggcgatgac gtcgcccacg
     4981 gtgaacccgg ccatcaggcg tcctctccct cggagtccga gccgaccagc agcacgctcg
     5041 tcgcggtgac gacgtgctcg ccggccgcat cggtgatggt cgcgtcactc gtgatcatcg
     5101 cgttgccgcc gagcgcgcgg atgccggtga ccgacagctg cgcggtgagc tcgtcacccg
     5161 cgacgatcgg gcgcgagtag gtgaagcgct gctccgcgtg gatggtgcga gccagcacga
     5221 tcccggagtc gggctcggcg agcagctgct gcagcgtgag gtcctggatc accatcgcga
     5281 aggtgggcgg agccacgacg tccgcgtagc cggccgcctg cgcggctgcg acgtcggcgt
     5341 gctgcggagc gtcggcgaag accgcgcgcg cgaactcgcg caccttctcg cgaccgacga
     5401 ggtagggggc cgtcggcggg aactcccggc cgaccagatc gttgttcacg ggcacccttc
     5461 gatcctatcc ggggccgctg ggcgtcgtgg cctgcgcgcc accgtgggaa ttctgctgtg
     5521 cgaacacgag cggacccctt gaccatcagc cagctcattg attaaatgcg attgtttctg
     5581 ccatcgaacg atcagaatca ctcaccacga ggagacgatg aagttgaact caccatcctg
     5641 ccgcccgcgc ttgcgtgcgc tcggcctcgc catcgccctg ggggtgtccg gcatcgggat
     5701 cgccggcacc gcatcggcgg catcagccgc atccgtcgcc cagacggtgg cgaaggcgtc
     5761 ctccgccccc accccgatca ctccccgacc cgccgctccc gaggtccccg tcgactcgac
     5821 cggcacctcg atcggctcga tcacggacgt gcagcgcgac ggagcgtcgg tcaccctcac
     5881 cgccgagcac ggcgccatgc gcgtgacctt cctcgaggac ggcaccttcc ggctcgaggc
     5941 cgacccgagc ggttccttca cggatcccgc gaacaccccg cagggcgatg cggcacgcac
     6001 cgctgacatc gtggtgggcg cgaagacgtt cggcggagcg gatgtcgcga tcgccgaggg
     6061 cgacgtgatc cgcatcgcgt cgaaggccgt cacggtctcg gtcgaccgcg ccacgggagc
     6121 cgtctccgcg acccgcgccg acggctcgac gatcttcgcg gagtccgccg cgatcacgtt
     6181 cggcaagagc tccgccaccc agcacctgaa gggccagaag ggcgagcaga tcctcggcgg
     6241 cggcatgcag aacggccgct cgatccacac cggatcggtc atcaacatcg ccaacaactt
     6301 cgactgggac gacgacggct acccgaacgc ggtgccgtac tacatgtcct ccgagggcta
     6361 cggcgtgctg cgcaacacct tcgcgaaggg cacgtacgac ttcgcacagc acaccaccac
     6421 gcaccaggag cgccggttcg acgcgtacta cttcgtgggc gactacgagc agtccctcga
     6481 gtcgtacacg cagctcaccg ggcggccgat gatgccaccg gtgtatgcgc tggagtacgg
     6541 cgacgcggac tgctacaacc gctccagccc cacgtacacc ggcaccagga cgccgtcgaa
     6601 gctgcgcacc ccgcaggcgc tcgacgtcgc caagggcttc gtcgagaacg acatgccggc
     6661 cggctggatg ctggtcaacg acggctacgg ctgcgagtac caggagctgc cggagaccgt
     6721 cgacgccatc caggagcaga ccgatctgaa gaccggcctg tggacccagc gctcgctgga
     6781 caagcaggag tacgaggtcg gtgaggccgg cgtgcggctg cgcaagctcg acgtggcgtg
     6841 ggtgggcagc ggctaccgtc tcgccctcac cggctgcgag gccgcccacg acggcatcga
     6901 gaagtactcg gatgcccgcg gcaccgcgct catggtcgag ggctgggccg gctcccagcg
     6961 ctgcggcatg cagtggacgg gcgaccattc cggaaacctc gacgccgtgc ggtggcagat
     7021 ctccgcgctg accggcgcgg gcaactcggg cctcgcgttc tccacgggtg acgtcgacgg
     7081 catcttcggc ggctcgccgg agagctacgt gcgcgacctg cagtggaagg cgttcgcccc
     7141 ggctctctac tcgatgagcg gctgggcctc ggtcgacaag cgaccgtggc tgtacggcga
     7201 cgaggcgacg aagatcaacc gcagctacct gcagctgcgc cagcgcctga tgccgtacat
     7261 ctacacgctg gccgccgagt cccaccgctc ggggctgccc atgatgcgct ccctggcact
     7321 ggagcacccg aaggaccccg gctcgtacag cgtcgaggcg aacaacgagt tcctgctcgg
     7381 cagcgactac ctggtcgccc cggtcttcac gaagaccgac gtgcgcaacg gcatctacct
     7441 gcccgccgga tcccagtggg tggacttctg gaccggcgcc gtgcacgagg gcggccaggt
     7501 gctgaacggg caccccgccc cgctggacag gctgccgatc ttcgtgcgcg ccggagcggt
     7561 ggtgccacag ggcatcaccg cccgcaacgc gtcgctcgtg ccggcgaact ctcccgtcac
     7621 gctgtcggtg ttcccgcagg gcgagagctc gttctccctc tacgaggacg accaggtcac
     7681 ccgcgcctac gcggacggcg cctccagcac ccagaagttc acggtgcagg ccccgggcgc
     7741 aggcaagaag ggctccgtca cgatcggaat cggcgagcgc gccggctcct acgacggcaa
     7801 ggccgacgcc cggccgtacg tgatcgaggc gcacaccggc tccgctccgg ccaacgtgtc
     7861 gatcgacggc aagggcctga agcaggccgc ttcccgcgaa gcgctggatg ccgcctcatc
     7921 cggctggttc ttcgatgcgg aggatgccgg cggcgtcatc cgcgtgaagg ttcccgccgt
     7981 ctcctcctcc gcatcggccg aggtcgtgct cgcagcgacc agcgccgtcg gcggacggga
     8041 cgccgacctc acctcggcat ccgtctcggt gactctcgac gaccgcgtgt tccagggcga
     8101 caccacttcg gtggaggcca ccttccgcaa caccggcacc aagaagaagg agaacgtgac
     8161 cctcaccccg cagctccctg agggctggtc gctggtctcc gcgaagggcg acaggacctc
     8221 cgtggtcgag cccggcgcct cggtgaccgc caccttcgag gcgaaggtca cggatgctgc
     8281 tgccgccgac ctgcagacgg tgagggtggt cgccgactac acgacacaga agaccgcgca
     8341 gcaggcgtcc ggggcgaacc agatctatgt cgcctacggg tcgctggccg gtgcgtacaa
     8401 cgccgacgcg gtgaccacga tcgacaccgc gaaggccggg gacttcgacg gcggcggcgc
     8461 cacgttctcg gccgaggcac tggcgcgggc cggcgtcact cccggatcga cggtcccggt
     8521 cggcaccggt gacgcggcga tcgcgtacac gtggccgagt ccgtccggcg cgaaggactc
     8581 ggtctccccg gccgggcaga ccatcgcgct gacgggcaag ggcacgcacc tcgcggtgct
     8641 ggcctccgcg gcatccggcg gtggcgtcaa ccctgcgctc gagctgcact acgccgacgg
     8701 cacgatctcg aagcagaacg tgttcttccc gaactggctg ccgcaggcat ccggactggg
     8761 cggcgccagc gtggccatca cgagcctcgg gcgtaacagc gcgaccaatc cgaacgtgta
     8821 cgagtacccc accggcaggt accaggtgta ctcgaacctc gtgcggctga acccggcgaa
     8881 ggagctcgcg tacgtcgtgc tgcccaccga gagccggctg aagatcttcg actggaaggt
     8941 cgtcgaccag ccgatgccgc cggcgccctc gggcacggtc tacgcatcgg acctgaactg
     9001 gatcagtgcg accaacggct ggggcgtgat cggcagggac gtcgccaaca aggactcggc
     9061 gacctcgccc gatcttccgc tggcgatcaa ctacatcgat ccggcgaccg gttcgagccc
     9121 gacctacgag aagggcctcg gcgtgcacgc cacatcgaag gtgacgtact acctcggtgg
     9181 caagtgctcg agcttcacgg catccgtcgg tctcgagaag gacttcgccg gcaacgtcat
     9241 cttcacggtc gacaccgatg ggaccaaccg ctaccagtcc cgcacgttca cgccgggctt
     9301 cgcgccggag gaggtgaacg tcgacctcac gggggtgcag tacatcgacc tcaacgtgac
     9361 cgctccgggc agcatcaacg gtgcccacgg agtgtgggga gacgcgctgt tccactgctg
     9421 atccgtcgga tgcgggcggc gtctcctccg ggaggcgccg cctgcgccgt gtccggggca
     9481 tgcgccgcgg cgagcgccgc cctgggaccc acccggtgct cagcggctgc ggatcgcctt
     9541 gatgctcatc tgcacggcga tcgcgacgag gtacgccgcg aacaggaggt tgcctgtcgc
     9601 cgggtcgacc agggtcgcca gccatgcccc gagagcggtc gtggtgcacg ccgagatccc
     9661 gatgatcagc gcgcccgcca tgtcgacgtt gcggtgccgc aggttgccga tcgtgccgga
     9721 gaccgcggtc gggatcatca tcagcagcga ggtgcccttg gcgaccaggt cgctggtccc
     9781 gaacaccacc atcagcacgg ggacgacgac cactcctccg ccgacgccga tcaggcccgc
     9841 gatgacgccg gtcaggatgc cgaccagcac cagtccggct ccgttcagcc aggtcaggtg
     9901 gaacaccgag tcgcgggagg ggatcaccag gaagaggctg accagcacca cgagcaggaa
     9961 ggcgacgaag ccccagcgca gcgcgcgctg cgagatcctc ggcatcagcc gcgatccgat
    10021 ctgcgcgccg gccacggctc ccgccgcgag gatgagcgcc gggatccagg cgaccgagcc
    10081 cgagagcgcg tacgagatca cgccgacgct cgccgtcggc acgatcgcgg tcagcgaggt
    10141 gccggccgcg agtcgctggt cgaagcccag cagcagcacg agcagcggca cgatcaccgt
    10201 gcccccgccg acgccgaaca gaccggagag gaggccggcg atgaggccga tcgccacgaa
    10261 cccggcgtac gcgcgaggcc cccgggtgtt ggcctgttca tcgcgcacga tgaacagact
    10321 atcccgggag ccccgtcggc cctgcggcgc gtgacgcctc tcagacctcc tggtcggcct
    10381 tgatcggcac cgcgatcgac tcggtcaggt gctgctcgta gcgcgtctgc ctgcggcgca
    10441 gccagagcgc accgaggatc agcaccacca gcacgccgta cgcgatcgcc gcgaacgggc
    10501 tcgcgatcag cgtcatcggg tcgccctggc tcagctgcag ggccttgcgc agctcgccct
    10561 cgcccatcgg gccgaggatc atgccgacga ccagcggtgc cacggggatg ccgtagcgcc
    10621 gcatgaagta gcccagcacg ccgatgatca gcaggatgat gatgtcggtg atgttgaact
    10681 tgatcgcgta cgcgccgaag gcggcgaaca tcaggatccc ggcgtacagg tacggtcgcg
    10741 ggatctgcag cagtttgacc cacatgccta ccagcggcag gttcagcacg atcaggatga
    10801 cgttgccgag gtacaggctc gccaccagcg cccacaccag cgcgggctgg ttggcgaaca
    10861 gcaggggccc cggctggatg ccataggact ggaacgcgaa caggatgatc gcggccgtcg
    10921 ccgtggtcgg caggcccagc gtcagcagcg gcacgagcac gccggcggcc gcggcgttgt
    10981 tggccgcctc cgggcccgcc acgccctcga tcgcgccgcg tccgaactgc tccttgtgct
    11041 tggagagctt ctgctcggcc gcgtaggaga ggaaggtcgc gacgtcggcg ccgccggcgg
    11101 ggatcgtgcc gatggggaat ccgatcgccg tgccgcgcag ccacggcttc cacgaccggc
    11161 gccagtcctc cttggtcatc cagttgctcc agcccttcgt gaccgggatg acatcgacgc
    11221 cgccgtgccg catcctcgac gcgacgtaca gggtctcgcc gacggcgaac aggcccaccg
    11281 cgacgagcac gacgctgatg ccctggttga gctcggggat gccgagggtg tagcgctgct
    11341 gcccggacag ggcgtccgtg ccgaccagcc cgatgaacag gccgatcgcg agcgacagca
    11401 cgccgcgcgg caccgacttg cccatcagcg cgccgaccgt gacgaacgcg accacgatca
    11461 gcgccagctt gtcgggattg ccgagcgaga ccgcgaagtc ggccaggatc ggcgcgagca
    11521 gcgtcagtcc gacggtcgcg atggtgcccg cgatgaacga gccgatcgcc gccgtcgcga
    11581 gcgcggcggc gccacgtccg agcttggcca tcttgttgcc ctcgatggcc gtgactatcg
    11641 aggcggattc gcccggtgtg ttcagcagga tgctggtggt cgatccgccg tacatgccgc
    11701 cgtagtagat gccggcgaag gtgatgatcg ccgcggtggc gtcgagcgtg taggtcagcg
    11761 gcagcagcag cgccacggtc atggccgggc cgatgccggg gagcacgccg acggccgtgc
    11821 cgatgaagac gccgatgaag gcccacatca ggtactcggg ctgcagcgcc gtggcgaagc
    11881 cctccaggag cagactccag ctgtccatca gaacatccct cccagccagc cgaacgcagg
    11941 accccagggc agcgagagcc ccaggaaccc gccgaacacg atctgcacga ccagcgcgac
    12001 gaccagtccg atgacgaagc ccagccacca gcgcttggca ccgagggccc aggcgacgat
    12061 gccgaacagg acggctgcgg cgggcgccca gccgatgagc tcgatgagca gcaggtgcac
    12121 gacgatcgct ccgacgatct tcgcgagcgt cacccagtcg gtgctggcat ccggatcgat
    12181 gtcctcgccc tcctcgacct ccgcgcgctc accgcgcagc acgccgatga ggacggcggc
    12241 cgccgagccg atgagcagga cagacacggc gatcgggaag acggtcgggc cgacctggat
    12301 gccgaccggg acgtggatgc tgaaggcgcc gaccagggcg aggacgccga gggcgagcat
    12361 gagcagggcg aagacgagct ctccgatggg aacccgtggg gcgggccgag tctcctcggc
    12421 ccgcccgttc gccccggtga tcggggcggt catgtcagat cagcccgatg ttcttgagcg
    12481 tgccctgcac gtcggagatg ttctgcttga cgaaggagtc cagctcggag ccggtgagga
    12541 aggcatcggc ccaccccttg gtctccagtt ccttcttcca cgcctcggag tcgtgcatct
    12601 cggtgacgat gcgctcgagc tcggcgcggt cggcgtcgga gatgtccccc ggtgcgatca
    12661 cgccgcgcca gttggtgagc accacgtcgt agccggcatc ggtgatggtc ggcacgtcgg
    12721 gcagctgctc gacgggcttc tcactggaca cggcgagcgc gcgcatcttg cccgcctcga
    12781 tgtgctgctg gaactcgccg acgccggaga ttccggcagc gaccttgccg cccaggatca
    12841 gcgagacggc ctcgccgccg cctgcgttgg gcgtgtagtt tagcttggcg gggatgtcgg
    12901 cgctcttcac cccggccgcg tccagcagga gcccggcgag gatgtggtcg gcgccgccgg
    12961 cggatccgcc ggtgacggtg atggcctggc ccttgtcgac gatgtcctcg accaggctct
    13021 tcaggtcctt gtacttcgag tcggccggca cgacgaccac gaggggctcg tcggtgaggc
    13081 gggcgatcgg cgtggtgtcc tccaggcgca ccttggacgc gttggtctcg atggcgccga
    13141 ccatgaccag gccgttgacc atcagggtgg cgggatcctt ctcggtggca agctgggcga
    13201 ggccgatcgt gccgcccgcg cctccgacgt tggtgaccgg agcggtcttc acgatcttgt
    13261 cctcggtgag caccttggcc atcgtgcgac cggtctggtc ccagccgccg cccgggtcgg
    13321 ctgggacgac gatggagacg ttcgagaagc tggacttggc gtccttgccg ccggcatccg
    13381 tcttgtcgcc tccgccgttc tgtgccgagc atccggcgag tgccagggct gcgatggccg
    13441 cgatcgcgac gatcgcgtta cgggacttct tcaggcgctg attcatcatg cgctcctcct
    13501 tgagcggtgt agggaatgac gcaacgctat gcatcgcggc gccgccgcca tcgcttcgga
    13561 aaccattggt cgcgtttcga aagtattggt cgtggatgcc tgcggaaggc ggttccgggc
    13621 gtgcgcgagt gcggcgagaa tggatccgtg gcctcgagga tgaagcacgc atcgaagaag
    13681 atgcgcgaac gcaggatgac tctgcgcgtg cagctgatcc tgctgcaggc cctgatcgtg
    13741 gccgtcgtca cgctcatcgc cggggtgact gcggggatca tccaggcgca caccgtgcgg
    13801 gatgcgtacg aggaccggat gctggcggtc gcgcagtcca tcgcatccat gccgctcatc
    13861 cgtgcggcgt tcgacgacga ggacccgtcg gcgaccattc agccgctcgc cgaagccatc
    13921 cggcgcgcct cggacctcac ctacgtggtc gtggcgaacg ccgacggcat ccgctactcg
    13981 catccgaacg aggccctcat cggccagaag gtctccaccg atccgtccgt gccgctgtcc
    14041 gggaagatct acatgggcac gcagaccggc acgctgggtc cgtcgtggcg cgtcaaggtg
    14101 ccgatcttct cggacgactc gcaggtcatc ggcacggtct cggtgggcat cctggagtcc
    14161 cagggcgacg gcgagttcgc cgtctacctg ttctggatca tcggcgccat gctcggcgcg
    14221 gtgatcctcg gggtcttcgg ctccgcctgg gtgacggcgg tgatccgccg ccgcatccat
    14281 ctgctcgagc ctcatcagat cacggcgctc gtgcgcaatc aggagactac gctgcacggc
    14341 ctcagcgagg gcgtgatcac cgtcgacgcg cacgggatcg tcacgctggc gaacgacgcc
    14401 gccgcccggc tgctcggcac ggacgccgag acgctcaccg gctcccccgc cgccgacgtg
    14461 ctcggcgatg acctcatggc cgtgctgcat gacggcgagg atgcggggcg cccggtcatc
    14521 gtgggcagca gcgt
//
LOCUS       DATMSX010000217         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_78630,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000217 DATMSX010000000
VERSION     DATMSX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78630"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VN041_06765"
     CDS             <1..493
                     /locus_tag="VN041_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWU28782.1"
                     /translation="ALQAIRPLRTVRLIGRDVGRAAAAVEEARTFAPGAEITVGTPDA
                     VRTADLIVCATTASDPLFHSDDVRDDATVVAIGSHEPQKAELDPALVGRSQVVVESLR
                     VATSEAGDVIRAIEAGALSEGDLVTMHDLFTGTVAPATDRPRIVKTCGMGWQDLAVAR
                     LAV"
     gene            526..1509
                     /locus_tag="VN041_06770"
     CDS             526..1509
                     /locus_tag="VN041_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HWU28783.1"
                     /translation="MHWSTEDWHTAGEPFRIVENVPTHGATVAERRVEAITGEADGIR
                     RFLCLEPRGHDDMYGGFITPGDDEGADFGVLFWHKDGFSTACGHGTIALGAWAVRTGR
                     VPSDPDGETVVTIDVPSGRVQARVHQSGGRIEDIVFRNVESRVLERGIPLSTSRGDVV
                     VDLVFGGAVYATLPASALGLEVAPEHTADLIAIGREIKWALNEHPSAQLEDSRLSGVY
                     GTILFDDLGRTAEGPHQRNVTVFADGEVDRSPCGSGTAARVALLAATGELADGEVLTH
                     DSIIGTRFLARIAERTSLGVIPEVSGIAHRVGTSTFELDEGDPLPSGFSLR"
     gene            1529..2716
                     /locus_tag="VN041_06775"
     CDS             1529..2716
                     /locus_tag="VN041_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU28784.1"
                     /translation="MRTGSLIITGAQVWDGAGFTARDVHIVDGRVVSTSEADADAERL
                     DASGRWLIPGLIDAHFHAYATSMDGLENERGPLSFTAINGARRLTRALQRGFTTVRDV
                     AGGDIGLAQAVDADLFPSPRYHFTGPALSQTGGHGDPRSAHVDVCFSHGHMCEVVDGV
                     DNLRLAVRNRMRTGAHAIKVMASGGVFSLVDPIRNPQYSSEELRAVVDEATRRGSYVC
                     AHAYSSEAVQHAVAAGIRSIEHGNLIDQATAELMAEAGAFLVPTLAAYDAMDRRGEEL
                     GLNETSLAKNAVVLEKGQDAVRLAHAAGVRVGFGTDLMGDLEDDQLAGIRLQVAASSA
                     AETLRSMTSVNAELIGDDALGHLGEGAYGDAVLLASDPLADPSALWEQDARVAVVRAG
                     VVV"
     gene            2861..3304
                     /locus_tag="VN041_06780"
     CDS             2861..3304
                     /locus_tag="VN041_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HWU28785.1"
                     /translation="MSEITIRPAAADDHPALVRIWRSAVDATHDFLAEADRDAIESRL
                     ASDYFPAVTLVIAEVEDSPIGFAGALNGNLEMLFVDAAHRGAGIGTLLLEHAIAHHAV
                     SRVDVNEQNPSAAAFYDRRGFEVIGRSELDDAGRPYPLLHLRLRG"
BASE COUNT          533 a         1317 c         1213 g          494 t
ORIGIN      
        1 ggcgctccag gccatccgcc cactgcgcac cgtccgcctg atcggccgcg acgtcggtcg
       61 cgccgcggca gccgtggagg aagcccgcac gttcgcaccc ggtgccgaga tcaccgtcgg
      121 aaccccggat gctgtgcgca ccgccgacct gatcgtgtgc gcgaccacgg catccgatcc
      181 cctcttccac tccgacgacg tgcgcgacga cgcgaccgtc gtcgcgatcg gctcgcacga
      241 accgcagaag gccgagctcg accccgcact ggtcggacgc tcgcaggtcg tcgtcgagag
      301 cctgcgcgtc gcgacctcag aggccggcga cgtgatccgc gccatcgagg cgggcgcgtt
      361 gagcgagggc gacctggtca ccatgcacga cctgttcacc ggtaccgtcg ctccggcgac
      421 cgaccggccg cgcatcgtga agacctgcgg catgggctgg caggacctcg ccgtcgcccg
      481 cctggccgtc tgagccggcc ggcccgaatt ccgagaggac cccgaatgca ctggagcacc
      541 gaggactggc acacggcagg cgagccgttc cgcatcgtgg agaacgtgcc cacgcacggc
      601 gcgaccgtcg cagaacgccg cgtcgaggcg atcacgggcg aagccgacgg catccgccgc
      661 ttcctctgcc tggagccgcg cggacacgat gacatgtacg gcgggttcat cacccccggc
      721 gacgacgagg gcgccgactt cggcgtgctg ttctggcaca aggacggctt ctccacggcg
      781 tgcgggcacg gcacgatcgc tctgggcgcg tgggcggtgc gcacgggtcg cgtgccctcc
      841 gaccccgacg gcgagaccgt cgtgaccatc gacgtgccca gcggccgcgt gcaggcgcgc
      901 gtgcaccaga gcggcggacg catcgaagac atcgtgttcc gcaacgtcga gtctcgcgtg
      961 ctcgaacgcg gcatcccgct gagcacctcc cgcggcgacg tcgtcgtcga tctggtgttc
     1021 ggcggcgccg tgtacgccac gctcccggca tccgccctcg gcctcgaggt ggcccccgag
     1081 cacaccgccg acctcatcgc gatcggccgc gagatcaagt gggcgctcaa cgagcatccg
     1141 tccgcgcagc tggaggattc ccgcctcagc ggcgtctacg gcaccatcct gttcgacgac
     1201 ctcggccgca ccgcggaggg cccgcatcag cgcaacgtca ccgtcttcgc cgacggcgag
     1261 gtggaccgga gcccgtgcgg ctccggcacc gccgcccgcg tcgcgctgct cgcggcgacc
     1321 ggcgagctcg ccgacggtga ggtgctcacg cacgactcga tcatcggcac gcgcttcctg
     1381 gcgcggatcg ccgagcgcac cagcctcgga gtgatccccg aggtcagcgg gatcgcgcac
     1441 cgcgtcggga catcgacgtt cgaactcgac gagggcgacc cgctcccgtc cggattcagc
     1501 cttcgctgac ttgtctgagg agtaacccat gcgcacagga tcgctcatca tcaccggcgc
     1561 ccaggtctgg gacggcgcgg gcttcacagc ccgcgacgtg cacatcgtcg acggccgggt
     1621 cgtctccacc tcggaggcgg acgcggatgc cgaaaggctg gacgcttccg gccgctggct
     1681 gatccccggc ctgatcgatg cccacttcca cgcctatgcg accagcatgg acggcctcga
     1741 gaacgaacga ggcccgctca gcttcaccgc gatcaacggc gcccgtcgcc tgacccgtgc
     1801 gctgcagcgc gggttcacca ccgtgcgcga cgtcgccggc ggggacatcg gcctggcaca
     1861 ggcggtggat gccgatctct tcccctcccc gcgctaccac ttcacgggtc cggcactcag
     1921 ccagaccggt ggccacggcg acccgcgatc cgcacacgtg gacgtgtgct tctcgcacgg
     1981 acacatgtgc gaggtcgtcg acggcgtgga caacctgcgc ctggccgtgc ggaaccggat
     2041 gcgcaccggc gcgcacgcca tcaaggtgat ggcatccggt ggcgtgttct cgctggtcga
     2101 cccgatccgc aatccgcagt actcctcaga ggagctgcgc gcggtggtcg acgaggccac
     2161 ccgccgcggc agttacgtgt gcgcgcacgc ctactcctcc gaggcggtgc agcacgccgt
     2221 cgccgccggc atccggtcga tcgagcacgg caacctgatc gatcaggcga cagccgaact
     2281 gatggcggag gcgggcgcct tcctcgtgcc gacgctcgcc gcctatgacg ccatggaccg
     2341 taggggcgag gagctcggac tgaacgagac ctcactcgcg aagaacgcgg tcgtcctgga
     2401 gaagggtcag gatgcagtgc gcctcgcgca cgccgccggc gtccgcgtcg gattcggcac
     2461 cgacctgatg ggcgacctcg aggacgacca gctcgccggc atccgcctgc aggtcgcggc
     2521 gtcgagcgcg gccgagacgc tgcgctccat gacctctgtg aacgccgagc tcatcggcga
     2581 cgacgctctc ggacacctgg gcgagggcgc ctacggcgac gccgtgctgc tggcatccga
     2641 tcccctcgcc gatccgagcg ccctgtggga acaggatgct cgcgtcgccg tggtgcgtgc
     2701 gggggtcgtg gtctgactct gcgctcgagt gcaccgtctg ccgacgactg caccgctcag
     2761 cgacgtcggc aggcggtgca ctcgacgaca agtggtgcac tcgagcgtgc ggggcagcgg
     2821 cgcgggcgcg ggcgcagcgc gcatcgcgta gcgtcgaggg gtgagcgaga tcaccatccg
     2881 gccggccgcg gccgacgacc accccgccct ggtgcgcatc tggcgcagcg ccgtcgatgc
     2941 cacgcacgac ttcctcgccg aggctgatcg cgacgcgatc gagtcgcgtc tggcgtccga
     3001 ctacttcccc gccgtcaccc tggtcatcgc cgaggtcgag gactccccca tcggcttcgc
     3061 cggcgcgctg aacggcaatc tggagatgct gttcgtggat gccgcacacc gcggcgcggg
     3121 gatcggaaca ctcctgctcg agcacgcgat cgcccaccac gcagtgagcc gggtcgacgt
     3181 caacgaacag aacccgtcgg cagcggcgtt ctacgaccgc cgcgggttcg aggtcatcgg
     3241 ccgcagcgag ctcgacgacg ccggccgccc gtacccgctg ctgcatctgc gcctgcgcgg
     3301 ctgaccgagc tcccttgtag agcattgccg cccctgctcg gcgtcgccgc ccctgtccag
     3361 ttgcgcaact gaacaggggc ggcggcgaca ggcaggggcg ggaccgcgtc gaaacccggc
     3421 tcagcgcacc gaagcgcccc acgaggtcga gttgccggcc cggctgatgc tctgcggcac
     3481 accccagggg ttcgcgtcct gcagcggcgc gggcaggagc tcctgcgggg caccctggta
     3541 ggccacgccg cgcagga
//
LOCUS       DATMSX010000218         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135642,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000218 DATMSX010000000
VERSION     DATMSX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135642"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VN041_06785"
     CDS             complement(<1..846)
                     /locus_tag="VN041_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HWU28786.1"
                     /translation="MSDDNAPKPTPPIPAPPRKPMPRVTAAPAAAPTVPAPVSSDASE
                     WGRVAEDGTVEVREGDDWRPVGQYPDGTPDEALAYYARKFDDFAVKLGTLETRAQGGG
                     SSASDLTKQAKHLLDEVTGAAAVGDLAGLRTRIETLISSLSEAAAKEAEAAKEALDAA
                     IAERTVIVEKAEAIAGRDLAKVQWKQVTVEMTELFDAWQTHQQNGPRLPKSTAQQLWK
                     RFRDARSTVDKARRAFFSELDDVHRAARDVKSRLVERAEALAPRGIDGIPAYRTLLDE
                     WKAAGR"
     gene            complement(905..1210)
                     /locus_tag="VN041_06790"
     CDS             complement(905..1210)
                     /locus_tag="VN041_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28787.1"
                     /translation="MATRGSRGAQQRARTDAERARLYAARRDWNDGLVARRRRDIVIA
                     VVAGGLIVIGAGVSQAVHAQVSAPAPKPSPTATEPAPTPLLTPPADGAPAPTQTPGE"
     gene            1365..3959
                     /gene="nirB"
                     /locus_tag="VN041_06795"
     CDS             1365..3959
                     /gene="nirB"
                     /locus_tag="VN041_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HWU28788.1"
                     /translation="MEQGAMTEQLSAAADVLVVGAGMVAHRFVDSLLSDGKDRHSVTV
                     IGEEPRDPYDRVGLTSFFSGATVDELTLDRRVFADPRVTHLSGDPVTRVDRGARTVQT
                     RSGAVLGYGTLVLATGSYAPRLPVEGRDLDGCFVYRTLDDVTRLREYVALRSARLGRT
                     LRGAVIGGGLLGLEAAGALQGMDVDCTVVQSSDRLMSAQLDAAGGTVLRRLIEARGIS
                     VRTRSRTSRLTADDSGAVTAIEFAHDGYLPADVVVFTVGVRPRDELARASGLAVHPDG
                     GVIIDDGCRTDDPRILAVGEVANFEGRAVGLVAPGYAMAEVAAARLNGADASFPGYDD
                     STKLKLSGVDVASFGDAMAEIPGALDVVYTDPVAGVYKKLVLSDDAKTLLGGILIGDA
                     SAYGALRPLVGGPLGGDPAGYLLPEDRMDAPSRELSDDAVVCSCSNVTAGRIRTAVHE
                     EGCADAASVKSCTRAGATCGSCVFMVSKIVAKELAKSGRTASTALCEHFDMSRRQLFD
                     AVRVSELTTFSAIIARFGRGRGCDICKPALASILSVLVGEHVLEGENAALQDTNDHVM
                     ANMQKDGTYSVVPRMPGGEVTPAGLIAIGRIADDYGLYTKITGGQRIDMFGARLEQLP
                     AIWGRLVEAGFESGQAYGKALRTVKSCVGSTWCRYGVLDSVGMAVRLELRYRGLRAPH
                     KLKVGVSGCARECAEARSKDVGVIATETGWNMYVGGNGGFSPRHAELLASELDDEGLI
                     RAIDRFFMYYIRTADRLQRTAPWCEELEGGLDGLREVIFDDSLGICADLDAAMARHVD
                     RYEDEWAATLRDPEKLRRFGSFVNAADTPDPSLAYVPVRGQVRPATADERSAESILIA
                     GTTLEVRR"
     gene            3956..4318
                     /gene="nirD"
                     /locus_tag="VN041_06800"
     CDS             3956..4318
                     /gene="nirD"
                     /locus_tag="VN041_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWU28789.1"
                     /translation="MTIAATVDTLVRVCSVRDLEVERGRAALFGSTQVALFLLADGTV
                     HAVSNLDPYSGAHVMSRGIVGTRDQSPTVASPIHKQVFDLRTGLCVATQGRHPASLRV
                     WPVVVRDEEVYLDFEEQG"
     gene            4315..>5328
                     /gene="cobA"
                     /locus_tag="VN041_06805"
     CDS             4315..>5328
                     /gene="cobA"
                     /locus_tag="VN041_06805"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWU28790.1"
                     /translation="MTLMLGVSLAGRAVLVVGGGAVSARRLRRFLDEGAVVRVVAPDL
                     HPDTARLVRTHALSWQARRFRAADLAGMWMAHVATGDANVDRRIVALCERRRVLCVAA
                     GDGSQGSARMTAQTVVGDVTLGVVSTHGADPRRTMAIRDAIGDLVANGRLPLRRNRRG
                     GAGRVDLVGGGPGPVDLMTVRARRLIAEADVIVTDRLGPAQEVVRELDPAVQVIDVGK
                     RPGAHPVPQEEINSILVEHALEGRRVVRLKGGDPFVFGRGGEEMQACLAAGVPVEVTP
                     GVSSAIAVPQAAGIPVTHRGTAAAFHVVNGQGGLEPSTLAAMADPTVTTIVLMGVAAL
                     PRLV"
BASE COUNT          732 a         1806 c         1950 g          840 t
ORIGIN      
        1 gcggccggcc gccttccact cgtcgagcag ggtgcggtag gcggggatcc cgtcgatgcc
       61 gcgcggagcg agggcctcgg cgcgctcgac cagacgggac ttcacgtcgc gtgcggcgcg
      121 gtgcacgtcg tcgagctcgg agaagaacgc gcggcgcgcc ttgtccacgg tcgaacgggc
      181 atcgcggaac cgcttccaga gctgctgcgc cgtgctcttg ggcaggcggg gtccgttctg
      241 ctggtgcgtc tgccatgcgt cgaacagctc ggtcatctcg accgtgacct gcttccactg
      301 caccttggcg aggtcgcggc cggcgatggc ctcggccttc tccacgatga cggtgcgctc
      361 ggcgatcgcg gcgtccagcg cctccttggc ggcttccgcc tccttcgcgg cagcctcgga
      421 gagcgagctg atcagcgtct cgatgcgcgt gcggagtcct gcgaggtcgc cgaccgcggc
      481 ggcaccggtg acctcgtcga gcaggtgctt cgcctgcttg gtcagatcgg acgccgagct
      541 tccgccgccc tgcgcccggg tctccagcgt gccgagcttg acggcgaagt cgtcgaactt
      601 gcgcgcgtag taggcgaggg cctcgtcggg cgtgccgtcc gggtactgtc cgaccggacg
      661 ccagtcgtcg ccctcacgca cctcgacggt gccgtcctcg gcgactcggc cccactcgga
      721 tgcatccgag gagaccggcg cagggaccgt gggggctgcg gcgggagcgg cggtgacccg
      781 cggcatcggc ttgcgcggcg gtgcggggat cggcggggtg ggcttgggtg cgttgtcgtc
      841 agacacggtg ttctccttgc gcggtctgtc cgcgggagtg ggaaaggact ccgctcagcc
      901 tagttcactc gccgggagtc tgcgtgggtg cgggcgcccc gtcggcgggc ggggtcagca
      961 gtggtgtggg cgcgggctcg gtcgccgtcg gagacggttt cggggcgggt gcgctcacct
     1021 gcgcgtggac cgcctgactc actccggctc cgatgacgat gagaccgccc gcgacgacgg
     1081 cgatcacgat gtcacggcgt ctgcgggcga cgaggccgtc gttccagtcg cggcgtgccg
     1141 cgtagagacg tgctcgttcc gcatccgtgc gtgcgcgctg ctgcgcgcca cgggaaccgc
     1201 gtgtcgccat cgagttcctc tccctccgag cgggccgggg cctgccaccg caaccctatc
     1261 gttcggtctg ctcacaccat cgttacccgt ttgtgagcga actgttcgac gcgcgtaacg
     1321 cggatcggcc ggttcgcgaa acacgggctc catagcttcg atgcatggaa cagggtgcga
     1381 tgaccgaaca gctcagtgcc gccgcggatg tgctcgtggt gggtgcgggc atggttgcgc
     1441 accgcttcgt cgacagtctt ctcagcgacg ggaaggaccg gcactccgtc accgtgatcg
     1501 gcgaggagcc gagggatccc tacgaccgcg tgggcctgac gtcgttcttc tccggtgcga
     1561 cagtcgacga gctcacgctg gaccgcaggg tgttcgccga cccccgggtg acccatctga
     1621 gcggagatcc cgtgacgcgc gtcgaccgtg gcgcccgcac ggtccagacc cgctccggcg
     1681 cggtgctcgg ctacggcacg ctggtgctcg ccaccgggtc gtatgccccg cggcttccgg
     1741 tcgagggccg cgaccttgac ggatgcttcg tctatcgcac cctcgacgac gtgacgcggc
     1801 tgcgtgagta cgtcgcgcta aggagcgcgc gactggggcg gacgctccgc ggtgcggtga
     1861 tcggcggcgg gctgctcggg ctcgaggctg cgggggcgct gcaggggatg gacgtggact
     1921 gcacggtggt gcagtcgtcg gatcggctca tgtccgccca gctcgacgcg gcgggcggga
     1981 ccgtgctgcg gaggctcatc gaggcacgag gcatctccgt ccgcaccaga agccgcacca
     2041 gcagactcac ggccgacgac tccggcgccg tcaccgcgat cgagttcgcg cacgacggct
     2101 accttcccgc cgacgtcgtg gtgttcacgg tcggcgtccg tccgcgcgac gaactcgctc
     2161 gcgcgtcggg actcgcggtc cacccggacg gaggcgtgat catcgacgac ggatgccgca
     2221 cggacgatcc tcggatcctc gccgtcggtg aggtcgcgaa cttcgagggt cgcgcggtgg
     2281 gtctggtcgc gcccgggtac gcgatggcgg aagtggcggc cgcgcgactg aacggtgcgg
     2341 acgcgtcgtt ccccggatac gacgactcga cgaagctgaa gctgtccggc gtggatgtcg
     2401 cgagcttcgg agacgcgatg gcggagatcc ccggcgcgct cgacgtggtc tacaccgatc
     2461 cggtggcggg cgtctacaag aagctcgtcc tctcggacga cgcgaagacg ctcctcggag
     2521 gcatcctgat cggcgacgca tcggcgtacg gcgcactgcg tccgctcgtc gggggcccgc
     2581 tgggcggcga ccccgcgggg tatctcctcc cggaggaccg catggatgcg ccctcccgtg
     2641 agctgtccga cgacgctgtc gtctgctcgt gctcgaatgt cacagcaggg cgcatccgca
     2701 ccgccgtgca cgaggaggga tgcgcggacg cggcatcggt gaagagctgc acccgagcgg
     2761 gcgcgacctg cggttcctgc gtcttcatgg tgtcgaagat cgtcgccaag gaactcgcca
     2821 agtccggtcg cacggcttcc acggctctgt gcgagcactt cgacatgtca cgccgccagc
     2881 tgttcgacgc cgtgcgcgtg tcggagctga cgaccttcag cgcgatcatc gcgcgcttcg
     2941 gtcgcgggcg gggctgcgac atctgcaagc ccgccttggc cagcatcctc tccgtgctgg
     3001 tcggagagca cgtgctcgag ggcgagaacg ccgcgctgca ggacaccaac gaccacgtga
     3061 tggcgaacat gcagaaggac ggcacctatt cggtcgtgcc gcgcatgccg ggcggcgagg
     3121 tcacacccgc cgggctgatc gcgatcggcc ggatcgccga cgactacggg ctctacacca
     3181 agatcaccgg cggtcagcgg atcgacatgt tcggcgcgcg gctcgagcag ctgcccgcga
     3241 tctgggggcg cctggtcgag gcgggattcg agtcggggca ggcctacggc aaggcgctgc
     3301 gcacagtgaa gtcgtgcgtg ggctccacct ggtgccgata cggcgtcctc gactcggtgg
     3361 gcatggcggt gcgcctcgag ctgcgctacc gcgggctgcg cgcgccccac aaactcaagg
     3421 tgggcgtctc gggatgcgcc cgcgaatgcg ccgaggcgcg cagcaaggac gtcggagtga
     3481 tcgccaccga gaccggctgg aacatgtacg tcggcgggaa tggaggattc tcgccgaggc
     3541 acgccgagct gctggcctcg gagctcgacg acgagggact catccgcgcg atcgaccgct
     3601 tcttcatgta ctacatccgc acggccgatc gcctgcagcg gacggcaccc tggtgcgagg
     3661 agctcgaggg cggtctggac ggcctgcgcg aggtcatctt cgatgacagc ctcggcatct
     3721 gtgcagatct cgatgccgcg atggcgcgcc acgtcgatcg ctacgaggac gagtgggcag
     3781 cgacgctccg cgacccggag aagctgcggc gcttcggctc cttcgtgaac gccgcagaca
     3841 cccccgatcc ctcgctcgcc tacgtgcccg tccgcgggca ggtgcggccg gcgaccgcgg
     3901 acgagcgatc ggccgagagc atcctcatcg ccggaaccac cttggaggtg cgcagatgac
     3961 catcgcagca accgtcgaca cgctcgtgcg cgtgtgctca gtccgggacc tggaggtgga
     4021 acgcggccgc gccgcgctgt tcggcagcac gcaggtcgca ctgttcctcc tcgcggacgg
     4081 cacggtgcac gccgtgtcga acctcgaccc gtacagcggc gcgcacgtga tgtcccgcgg
     4141 catcgtcggc accagggatc agtcacccac cgtcgcttct ccgatacaca agcaggtctt
     4201 cgatctgcgc accggtctgt gcgtggcgac gcagggcagg cacccggcgt ccctgcgggt
     4261 ctggccggtc gtcgtccgcg acgaagaggt ctacctcgac ttcgaggagc agggatgacg
     4321 ctgatgctgg gggtctcact ggcaggacgc gcggttctgg tggtcggggg aggagccgtt
     4381 tcggcgcgcc gactgcgtcg gttcctcgat gagggggccg tcgtgcgcgt cgtcgccccg
     4441 gacctgcatc ccgacaccgc tcggctcgtt cgcacgcatg cgctgagctg gcaggcgcga
     4501 cggttccgcg ccgcggacct cgcagggatg tggatggcgc acgtggcgac cggcgacgcg
     4561 aacgtcgatc gccgcatcgt ggccctctgc gagcgacgcc gggtcctgtg cgtcgcggcc
     4621 ggtgacggct cgcagggctc agcgcggatg acggctcaga cagtggtggg tgacgtcacg
     4681 ctcggcgtcg tcagcaccca cggcgccgat ccgcggcgca cgatggcgat ccgggatgcg
     4741 atcggagacc tcgtggcgaa cggacgcctc cccctgcgcc gcaaccggcg aggcggcgca
     4801 ggccgcgtgg acctcgtcgg cggagggccc ggacccgtcg atctgatgac ggtgcgtgcg
     4861 cggcgcctca tcgccgaggc ggacgtgatc gtcaccgacc gtctcgggcc cgcccaggag
     4921 gtcgtccgcg aactcgaccc cgcggtgcag gtgatcgatg tcggaaagcg cccgggagcc
     4981 caccccgtgc cgcaggagga gatcaacagc atcctcgtcg agcatgctct cgaggggcgc
     5041 cgggtggtcc gcctcaaggg cggcgatccg ttcgtgttcg gccgcggtgg tgaggagatg
     5101 caggcctgcc tcgccgccgg tgtgccggtc gaggtcacgc cgggggtgtc cagcgcgatc
     5161 gcggtgccgc aggccgcagg catccccgtc acgcaccgcg gcaccgcagc cgccttccac
     5221 gtggtcaacg gccagggcgg actggagccg tcgacgctgg ccgcgatggc cgatccgacg
     5281 gtgacgacga tcgtgctcat gggcgtcgcc gcgctcccgc ggctcgtg
//
LOCUS       DATMSX010000219         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_97853,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000219 DATMSX010000000
VERSION     DATMSX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97853"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VN041_06810"
     CDS             complement(<1..820)
                     /locus_tag="VN041_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HWU28791.1"
                     /translation="MSYVKPAELVERMLDAGASKMQLSARDTVVRAFMGAALLTMGAA
                     FAVTVSTQTGQPLLGALLFPVGFAILYLFGYDLLTGVFTLGPLAVIARRPGATVPAML
                     RNWGLVLLGNAGGAVLVAVLMALYFTYSFSSEPSAVGQAISEIGHGRTVGYAEHGAAG
                     MLTLFVRGVLCNWLVSTGVVLAMASDSITGKVLGMWLPIMLFFYMGFEHSVVNMFLFP
                     SGLLLGADFTLWDYLVWNEFPTLLGNLVGGLLFVGLPLYLSHGRTRPRAAGSAAR"
     gene            995..>1633
                     /locus_tag="VN041_06815"
     CDS             995..>1633
                     /locus_tag="VN041_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU28792.1"
                     /translation="MTAAPLLSGQTPLAVRMRPVSLDEVAGQAHLLRTGSPIVALADP
                     DAKAPGAVSIILWGPPGTGKTTLAQAIARSSGRRFVELSAITAGVKDVREVMQEAITQ
                     RDLYGQTTILFLDEIHRFTKAQQDALLPGVENGWVILIAATTENPSFSVISPLLSRSL
                     LLTLQPLTDDDIGVLVDRAITDARGLSGAVTVADDARAALIRLASGDARRALT"
BASE COUNT          281 a          558 c          567 g          227 t
ORIGIN      
        1 ggcgcgccgc ggagcctgcc gcgcgcgggc gggtgcggcc gtggctcagg tagagcggga
       61 gcccgacgaa cagcagacca cccacgaggt tccccagcag ggtggggaac tcgttccaga
      121 cgaggtagtc ccacagggtg aagtctgctc cgagcagcag accggagggg aacaggaaca
      181 tgttgaccac ggagtgctcg aagcccatgt agaagaagag catgatgggc agccacatgc
      241 ccaggacctt gcctgtgatg ctgtccgacg ccatcgccag caccacgccc gtggacacca
      301 gccagttgca cagcacgccg cgcacgaaca gcgtcagcat gccggccgcg ccatgctccg
      361 catagcccac ggtgcggccg tgaccgatct cgctgatcgc ctgccccacc gcgctgggct
      421 cggaggagaa cgagtaggtg aaatacagtg ccatgagcac cgcgacgagg acggcgccgc
      481 ccgcgttgcc gaggagcacc agcccccagt tgcggagcat ggcggggacg gtcgcgcccg
      541 gcctccgggc gatcaccgcc agcgggccga gggtgaacac cccggtgagc aggtcgtatc
      601 cgaacagata gagaatggcg aagccgacgg ggaagagcag cgcgcccagc aggggctggc
      661 cggtctgcgt cgacacggtc acggcgaaag ccgcgcccat cgtgagcagg gccgcaccca
      721 tgaaggcgcg cacgacggtg tcgcgtgcgg agagctgcat cttgctggcg ccggcgtcga
      781 gcatgcgctc gacgagctcg gcgggcttga cataagacat gaggacctcc agtgacagga
      841 gcccgacggg ctcactcggg tccagcgtca ccggttcgcg tttcagggga tggcggatgg
      901 ggttacggca gtggaacgaa gaactcacct tccgccgttc gcgctgtgaa gtcgcgggcc
      961 tttgctgtcg gtgccgccca ctagcctgga gagcatgacc gccgctccgc tcctctcggg
     1021 tcagacgccg ctcgccgtgc gcatgcggcc ggtctcgctc gacgaggtgg cggggcaggc
     1081 gcacctgctg cgcaccgggt ctccgatcgt ggccctcgcc gatcctgacg ccaaggcgcc
     1141 cggtgccgtg tcgatcatcc tgtggggccc gcccggcacg ggaaagacca cgctcgcgca
     1201 ggcgatcgcg cgatcgtccg gccgacggtt cgtcgagctg tccgcgatca cggccggtgt
     1261 caaggacgtc cgcgaggtga tgcaggaggc gatcacccag cgggacctct acggccagac
     1321 cacgatcctg ttcctggatg agatccaccg cttcacgaag gcccagcagg acgcattgct
     1381 cccgggcgtc gagaacggct gggtgatcct catcgccgcg accaccgaga atccctcgtt
     1441 ctcggtcatc tcgccgctgc tgtcgcgttc cctgctgctc acgctccagc cgctgaccga
     1501 cgacgacatc ggagtgctgg tcgaccgcgc catcacagat gcgcgcggtc tgtccggagc
     1561 ggtcacggtg gccgacgacg cgcgcgccgc gctgatcagg ctggcctccg gcgacgcccg
     1621 ccgggcactg acc
//
LOCUS       DATMSX010000220         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_50595,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000220 DATMSX010000000
VERSION     DATMSX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50595"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..265)
                     /locus_tag="VN041_06820"
     CDS             complement(26..265)
                     /locus_tag="VN041_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28793.1"
                     /translation="MTDLSWRQADEDVFVATNRGEYAGFVTIEQNVHVVHDSYSRRIG
                     SYPTLAAARQALVDATRPPSRRRERLRRRILERRR"
     gene            593..1711
                     /gene="purM"
                     /locus_tag="VN041_06825"
     CDS             593..1711
                     /gene="purM"
                     /locus_tag="VN041_06825"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWU28794.1"
                     /translation="MAASPTNPYAQAGVDTAAGDLAVELMKASVRATHGPEVLGGVGG
                     FAGMFDASALLGYTRPLLASSTDGVGTKVAIAQAIDKHDTIGQDLVGMVVDDIVVVGA
                     KPLFMTDYIACGKVVPERIADIVRGIAEACSATGTALVGGETAEHPGLLGPRDYDVAG
                     AATGVVEADAVLGADRVQDGDAVIALASSGLHSNGYSLVRHIVANAGIGYGDNAADLG
                     TTWGEALLEPTRLYTTPLLKLIEELPAGAIHSLSHVTGGGIAANLARVLPQGSWSEVD
                     RSTWSPSPVFRVLADIAGTPITETEGTWNLGIGFLAVVAAEQKDAAIAAIEAQGIPAW
                     QVATVHTGARPAGEFEEGAKGVDGGAVRLVGAYANGTK"
     gene            1718..3184
                     /gene="purF"
                     /locus_tag="VN041_06830"
     CDS             1718..3184
                     /gene="purF"
                     /locus_tag="VN041_06830"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWU28795.1"
                     /translation="MCGIVGMVAGGPVNQDIYDALLLLQHRGQDATGIATAEPNGVMH
                     MAKAEGMVREAFRTRDMRSLLGNIGLGHVRYATKGTASSEEEMQPFYVNAPYGIVLIH
                     NGNLTNTRELTADMAQRDRRHLNSSSDTELLLNVLAHELQTTTSAVDLDPERIFEAVA
                     RTHQRIEGAYAVISIIAGYGLLAFRDPFGIRPLILGRRAGREDATKDEWVVASESLVL
                     ENADYEVVREVEPGEAVFITNEGELFTRQCAENPQLIPCAFEYVYLARPDSIMNGVSV
                     YESRLRMGERLADTIAKHVPLDEIDVVMPIPDSSRPSAMEVARKLGKEYREGFYKNRY
                     VGRTFIMPGQAVRKKSVRQKLNAMSAEFQGKNVLLIDDSIVRGTTSTQIIQMARDAGA
                     KSVIFASAAPPVRHPHVYGINMPSRAELVAHGRTIPEIAEELGCDRIVYQEVEDLKSA
                     IIEGTDLTDLDMSCFDGRYVTGTVTPEYLEWVEKSQSS"
     gene            3181..>3798
                     /locus_tag="VN041_06835"
     CDS             3181..>3798
                     /locus_tag="VN041_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28796.1"
                     /translation="MITSGARPLWQGRALAVLGILLCAFSLRSAVASLSPVIHEISAD
                     FPLSSAVLGLIGTLPPVCFAVFGLITPLLERRLGIERLTVIALCAMTAGLILRGLVAE
                     SVGLVLATAVIFAGVGMGNILMPPLVKKYFPDRIGLMMTLYTTAMACSTFVPPLVAVP
                     VADAVGWRFSLAMWAVFAAAGVIPWLIMLMRRTTDPAAPALTRPGI"
BASE COUNT          584 a         1313 c         1314 g          587 t
ORIGIN      
        1 gcgcggctcg gagggcgcgg ggcgctcagc gcctgcgctc gagtatgcga cggcgcagcc
       61 gctcgcgacg acgggacggg ggacgcgtgg catccaccag ggcctgacgg gcggccgcga
      121 gggtggggta gctgccgatg cggcggctgt agctgtcgtg caccacgtgc acgttctgct
      181 caatggtgac gaagcccgcg tactcgccgc ggttggtcgc gacgaagacg tcctcgtcgg
      241 cctgtcgcca ggagaggtcg gtcatctgtt cggtctgtgg ttcctgagag agcggagcga
      301 gacgaagggc gggcgcgtcg gagcgcgcgg aagtgtggat actgctcatg attcttgatt
      361 cgtgtgcggg cggcacgctg ccgcctccgc aatggccggg gagcggatcg catcggtgcg
      421 gtccagcctt gagagtgccc tggccggggc gtgcgctgcg ccgggtgaga gtcggcgaaa
      481 acttgcgcaa ccacatactc tagcacgtct ggtggatcag catccgtcgc gacggcatcc
      541 ggcgggtgca tccgcgacct tcgtgacgcg cccggcccgg taggctgtac gggtggcagc
      601 ctcccccacc aatccctatg ctcaggccgg cgtcgacact gccgccggtg atctcgcagt
      661 cgaactcatg aaggcctccg tgcgcgcgac gcacgggccc gaggtgctcg gcggagtcgg
      721 cggattcgcc ggcatgttcg atgccagcgc cctgctcggc tacacgcgtc cgctgctggc
      781 gtccagcacc gacggcgtgg gcacgaaggt cgccatcgca caggcgatcg acaagcacga
      841 cacgatcgga caggacctgg tcggcatggt cgtggacgac atcgtcgtgg tgggcgcgaa
      901 gccgctgttc atgacggact acatcgcctg cggcaaggtc gtgcccgagc gcatcgccga
      961 catcgtgcgc gggatcgccg aggcgtgctc ggccacgggc accgcgctcg tgggcggcga
     1021 gaccgccgag catccgggtc tgctcggccc gcgcgactac gacgtcgcgg gtgcggccac
     1081 cggagtcgtc gaggcggatg ccgtgctcgg cgccgaccgc gttcaggacg gcgacgcggt
     1141 gatcgcactg gcatccagcg gtctgcactc caacggctac tcgctcgtgc ggcacatcgt
     1201 tgcgaacgcg gggatcggct acggcgacaa cgccgccgac ctcggcacga cctggggcga
     1261 ggcactgctc gagccgaccc gcctgtacac gacgccgctg ctgaagctca tcgaggagct
     1321 gcccgccggc gccatccact cgctgagcca cgtgaccggc ggcggcatcg ccgccaacct
     1381 cgcccgcgtg ctgccccagg gcagctggtc cgaggtcgat cggtcgacct ggtcgccgag
     1441 cccggtgttc cgcgtgctgg ccgacatcgc cggcactccg atcaccgaga ccgagggcac
     1501 gtggaacctc ggcatcggct tcctggccgt cgtcgcggcg gagcagaagg atgctgccat
     1561 cgccgcgatc gaggcgcagg gcatcccggc ctggcaggtc gcgacggtgc acaccggcgc
     1621 gcggccggca ggcgagttcg aagagggcgc caagggcgtc gatggtggag ctgtgcgcct
     1681 ggtcggcgcc tacgcgaacg gaacgaagta acaccccatg tgcggcatcg tcggaatggt
     1741 cgcaggcggc cccgtcaatc aggacatcta cgacgcgctg ctgctgctgc agcatcgcgg
     1801 gcaggacgcc accggcatcg ccacggcgga accgaacggc gtcatgcaca tggcgaaggc
     1861 cgagggcatg gtgcgcgagg cgttccgcac ccgtgacatg cgctcgctgc tcgggaacat
     1921 cggcctgggc catgtgcgct acgccacgaa gggcaccgcc tccagcgagg aggagatgca
     1981 gcccttctac gtgaacgccc cctacggcat cgtgctgatc cacaacggca acctcacgaa
     2041 cacgcgtgag ctgaccgccg acatggcgca gcgcgaccgc cgtcatctga actcctccag
     2101 cgacaccgag ctgctgctga acgtgctcgc gcacgagctg cagaccacca cctccgccgt
     2161 cgacctcgac ccggagcgca tcttcgaggc ggtcgcccgc acgcaccagc gcatcgaggg
     2221 cgcctacgcc gtgatctcga tcatcgccgg gtacggcctg ctcgccttcc gcgacccgtt
     2281 cggcatccgt cccctgatcc tgggccgccg ggccggtcgg gaagacgcga ccaaggacga
     2341 gtgggtcgtc gccagcgagt cgctcgtgct cgagaacgcc gactacgagg tggtgcgcga
     2401 ggtcgaaccc ggcgaagccg tgttcatcac caacgagggc gagctgttca cccggcagtg
     2461 cgccgagaac ccgcagctga tcccgtgcgc tttcgagtac gtgtacctcg cccgccccga
     2521 ctcgatcatg aacggcgtct cggtctacga gtcgcgcctg cgcatgggcg agcggctggc
     2581 ggacaccatc gccaagcatg tgccgctcga cgagatcgac gtggtcatgc cgatcccgga
     2641 ctcctcgcgc ccttcggcca tggaggtcgc ccgcaagctc ggcaaggagt accgcgaggg
     2701 cttctacaag aaccgctacg tgggccgcac gttcatcatg cccggacagg cggtgcgcaa
     2761 gaagagcgtg cgccagaagc tgaacgccat gtcggcggag ttccagggca agaacgtgct
     2821 gctgatcgac gactcgatcg tgcgcggcac caccagcacc cagatcatcc agatggcgcg
     2881 cgacgccggt gccaagtctg tgatcttcgc ctcggcggcc ccgcctgtcc gccacccgca
     2941 cgtgtacggc atcaacatgc cctcccgcgc tgagctggtc gcgcacggcc ggacgatccc
     3001 cgagatcgcc gaagagctcg gctgcgaccg catcgtgtac caggaggtcg aagacctcaa
     3061 gtccgcgatc atcgagggca ccgacctgac cgacctcgac atgagctgct tcgacgggcg
     3121 ctacgtcacc ggaaccgtga cccccgagta cctggagtgg gtcgagaagt cgcagtcgtc
     3181 gtgatcacgt cgggcgcacg gccgctctgg caggggcgcg cgctcgcggt gctcggcatc
     3241 ctgctctgcg ccttctcgct gcgttcagcg gtggcatccc tctccccggt gatccacgag
     3301 atctcggcgg acttcccgct gtcctcggcg gtgctcgggc tcatcggcac gcttccgccg
     3361 gtgtgcttcg cggtgttcgg cctgatcaca ccgctcctgg agcgccggct cggcatcgaa
     3421 cgactcacgg tgatcgcgct gtgcgcgatg accgcggggc tgatcctgcg cggcctggta
     3481 gcggagtcgg tcggtctggt cctcgcgacc gcggtgatct tcgcgggcgt ggggatgggc
     3541 aacatcctca tgccgccgct ggtgaagaag tacttccccg accgcatcgg cctgatgatg
     3601 acgctgtaca ccaccgcgat ggcgtgctcg acgttcgtgc cgccgctggt cgccgtgccg
     3661 gtggcggatg ccgtgggctg gcgcttctcg ctggccatgt gggccgtgtt cgccgccgcg
     3721 ggagtgatcc cgtggctgat catgctgatg aggcgcacga ccgatcccgc cgcccccgcc
     3781 ctgacccgcc cgggcatc
//
LOCUS       DATMSX010000221         6913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_126604,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000221 DATMSX010000000
VERSION     DATMSX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126604"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1677)
                     /locus_tag="VN041_06840"
     CDS             complement(217..1677)
                     /locus_tag="VN041_06840"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWU28797.1"
                     /translation="MENVVTHHDDWHINEELAERMIPLIGGLNRKHDVVTSLHGHRLL
                     GLSTTGILEVHERVAQLGHSHLALTDTLAVLEALREIGPGASSLDVARLVEGHAASGM
                     DLVAYLRGELAPALDAELSEPVDVVLYGFGRIGRLLARIIIAHKGGGSGLRLRAIVVR
                     KGAENDLVKRASLLLRDSVHGRFEGTVDVDEANSQIIANGTRIQVIYSDDPGTIDYTA
                     YGIRDAIVVDNTGRWRDEAGLSRHLESTGVARVLLTAPGKGELKNIVHGINDSTIEPG
                     DRILSAASCTTNAITPVLAAMNEAYGIVRGHVETVHSFTNDQNLIDNFHKGDRRGRSA
                     VLNMVITETGAAKAVARALPELAGKLTGSAIRVPTPDVSLAVLHLTLENPATKDELND
                     YLRRASLHSKLRQQIDYVESPEVVSSDFVGSHRAGIVDGLATIANDKDVVLYVWYDNE
                     YGYSCQVVRVLEVMAGSHPVVLPARREVTLHEDVLV"
     gene            complement(1856..3004)
                     /locus_tag="VN041_06845"
     CDS             complement(1856..3004)
                     /locus_tag="VN041_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWU28798.1"
                     /translation="MPQLKQIAAEMQDVLRRPLGTAEARETVALLAGAAAFVIGFPVS
                     WLIFGGAHLAITGPGSIGWYAAVGGAIVAVLAFTAGRLAVRPSDAPADQPRDGFVGPA
                     DRLRWFDLVAIGGAYASIAFLGWLGIAQLLELSFIGAPVFAFPGAILVAVAFALTAYV
                     SFLGAARLTPMTLSLDLAVFLVVGAFAAMLTSSDPKWWQDNLSSLGQTTNSAAPAFNV
                     TLIISGVIVTTIARYATAGLPVDTRAQRRGRTLMRTGLVLVGIFLACVGVFPVDRFFL
                     VHNTVATGMAVVYAMLICALPWLLPSLPKVFFGFGYTYVAVIALLAIFFATGYYNLTA
                     VELIAAVLIFSWIIVFLRTASVAVQRDAPGAVERDPADASSPNEDAVR"
     gene            3095..3745
                     /locus_tag="VN041_06850"
     CDS             3095..3745
                     /locus_tag="VN041_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWU28799.1"
                     /translation="MTHSIRARAILLDMDGTLVDSTAVVERLWLRWSQAHGLDPRRVL
                     EVIHGRQGHQSMAILLPDRDHAINLAENAEMLATETADVEGVVEIPGAAGLLVALRDA
                     PHAIVTSADVALMTARMHAAGLTAPAMTVTAESVSASKPDPEGFLLGARMLGIEPADC
                     VVFEDSEAGVQAGLAAGMRVIGVGAHAHAHGATFVVDDLTHVQVRADEDGIELTLS"
     gene            complement(3789..4226)
                     /locus_tag="VN041_06855"
     CDS             complement(3789..4226)
                     /locus_tag="VN041_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU28800.1"
                     /translation="MSDLDAVDRAILDELRRDARTSMTAIADAVHISRAGAHARIKRL
                     TDAGVITGYTVRTDPVQLGHHASAYVMLAIEQSSWQDVQARLRAIREIEHMALVGGDF
                     DVILLVRASDARDLRRIVLEDIQAIPFIRSTRTTLIFEDFGRD"
     gene            4379..5482
                     /locus_tag="VN041_06860"
     CDS             4379..5482
                     /locus_tag="VN041_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWU28801.1"
                     /translation="MQHEDLLPRDTPVALIDKDGASVADEQYALPDAETLQRAYRGLV
                     EGRRINDQAYALVRQGRLAVYPSSHGQEACQVAASMVLGEHDWLFPTYRDSVAVISRG
                     VRPADAMVLLKGDWHSGYDPAEHRVAPQSTPLATQLLHAVGFAQAAKHRGEDTVVLAL
                     CGDGSTSEGDFHEALNFAAVFHVPVVFFVQNNEFAISVPLSRQTAAPSLAHKAIGYGM
                     PGQRVDGNDVAALLAVLGEAVDRARSGGGPSLVEAHTYRMQAHTNADDDTRYRDRDEV
                     KAWADRDPLSRMQVFLRAQGLLDDAAQQGIADGAEVIAAALRDALNADSDLDPEDLFR
                     FVTAERSPQLEEQWQVLRGEIERASLTDAGGHR"
     gene            5479..6516
                     /locus_tag="VN041_06865"
     CDS             5479..6516
                     /locus_tag="VN041_06865"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWU28802.1"
                     /translation="MTMANDIEIETPHVVGTMTMAAAINRALADAIAEDPDVVVFGED
                     VGALGGVFRITDGLAERFGEDRCFDTPLAESGIMGTAVGMAMNGMRPVVEMQFDAFAL
                     PAFEQVVSHVAKMGNRTRGAVRMPIVIRIPFGGGIGGVEHHCDSSEAYYAHTPGLTVV
                     SPSTPQDAYSLLRAAIASPDPVVFLEPKKLYWSKGEVDTSVTADLGTARIAREGTDVT
                     VLAYSAMVPVALEAAEIAASEGRSVQVIDVRSLSPFDDATVTAAVRSTGRAVVVAEAP
                     GYVSVASEIQARVFERCFEYLEAPVRRVTGFDTPFAPPKLEHWYLPDADRVLDAIDSL
                     HWDDDAEGAAR"
     gene            6513..>6913
                     /locus_tag="VN041_06870"
     CDS             6513..>6913
                     /locus_tag="VN041_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWU28803.1"
                     /translation="MSTTTARVFRLPDLGEGLTEAGLVQWLVKVGDTIATDQAIAEVE
                     TAKSIVELPSPFAGVITALHGEPGDVIDVGAPVLEVASDAPEAPTPTASDATEKDAYR
                     AEERAGSGNVLIGYGTTERSGSGRRRPRAVSA"
BASE COUNT         1090 a         2383 c         2415 g         1025 t
ORIGIN      
        1 gcccagcgac cggcgagcag gtcctggctc tgcacggcga gcatgtgccg cgcggcatcc
       61 accggagtcg cggcgggggc cgtcagccga tgcgagcgga gtcggcgctc ccgcagtgca
      121 cggggtgtca tccgtccatc atgccgggaa catccgacat cggcgcgccg cccggagcgc
      181 tctgaccccc ggacggcgcg acgcggtgtc acaccgtcag acgagaacgt cctcgtgcag
      241 cgtcacctca cggcgggccg gcaggacgac ggggtgggag ccggccatca cctcgagcac
      301 gcgcaccacc tggcaggagt agccgtactc gttgtcgtac cagacgtaca gcacgacgtc
      361 cttgtcattg gcgatcgtgg ccaggccgtc gacgatgccc gcgcgatgcg aaccgacgaa
      421 gtcgctggag acgacctccg ggctctcgac gtagtcgatc tgctggcgca gcttggagtg
      481 cagcgaggca cggcggaggt agtcgttgag ctcgtccttg gtggccggat tctcgagcgt
      541 caggtgcagc acggcgagcg acacgtccgg cgtggggacg cggatcgcgg agcccgtgag
      601 cttgcctgcg agctcgggca gcgcacgggc cacggccttg gcggcgccgg tctcggtgat
      661 gaccatgttg agcacggctg agcgcccgcg gcggtcgccc ttgtggaagt tgtcgatcag
      721 gttctggtcg ttggtgaacg agtgcacggt ctcgacgtgg cctcgcacga tgccgtaggc
      781 ctcgttcatc gccgccagaa ccggggtgat cgcgttggtg gtgcaggatg cggccgacag
      841 gatgcggtcg cccggctcga tcgtggagtc gttgatgccg tgcacgatgt tcttcagctc
      901 gcccttgccg ggcgccgtga gcagcacgcg ggcgacgcct gtggactcca gatggcggct
      961 caggcccgct tcgtcgcgcc agcggccggt gttatcgacg acgatcgcgt cgcggatgcc
     1021 gtaggcggtg tagtcgatcg tccccgggtc atcggagtag atcacctgga tgcgggtgcc
     1081 gttggcgatg atctgggagt tggcctcgtc gacgtcgacg gtgccctcga agcggccgtg
     1141 cacggagtcg cgcagcagca gggaggcgcg cttgaccagg tcgttctcgg cgcccttgcg
     1201 cacgacgatc gcccgcaggc gcagaccgct gccgccgccc ttatgggcga tgatgatgcg
     1261 ggcgagcagg cggccgatgc ggccgaagcc gtacagcacc acgtcgaccg gctcggaaag
     1321 ctcagcatcc agtgccgggg cgagttcgcc gcgcaggtat gcgacgaggt ccatgccgct
     1381 cgcggcgtgc ccctcgacca gtcgggcgac atccagcgag gacgcgccgg ggccgatctc
     1441 gcgcagcgcc tcgagcacgg cgagcgtgtc ggtgagggcg aggtggctgt gacccagttg
     1501 cgcgacgcgc tcgtgcacct cgaggatccc ggtcgtcgac aggcccagca ggcggtggcc
     1561 gtgcagcgag gtcacgacgt cgtgcttgcg gttcaggccg ccgatcagcg ggatcatccg
     1621 ctcggcgagc tcttcgttga tgtgccagtc atcgtggtgc gtcacgacgt tttccatgtg
     1681 cgtccttgcg agcgtgttgc agcatcgtcg tttcacgatg cggaggtggg agaggcggat
     1741 ccgccacgtc aaggctaacg tctgcctgcg catgaacctg tggttttcga cccgcaagaa
     1801 ctcgtctgga acccgggttc tgaaaagatc caaccttctt aagggcagga ctcgttcagc
     1861 gcacggcgtc ttcgttcggg gagctcgcgt ccgctggatc tcgctcgacg gcacccggcg
     1921 catcccgctg cacggccacg ctcgcggtgc gcaggaacac gatgatccag ctgaagatca
     1981 ggacggcagc gatcagctcc accgcggtga ggttgtaata gcccgtcgcg aagaagatcg
     2041 cgagcagggc gatcacggcc acgtaggtgt atccgaatcc gaagaacacc ttcggcaggc
     2101 tcggaagcag ccacggcagc gcgcagatca gcatggcgta gacgacggcc atgccggtgg
     2161 cgacggtgtt gtgcacgagg aagaagcggt cgacggggaa caccccgacg caggccagga
     2221 agatcccgac cagcacgagc cccgtgcgca tcagcgtccg cccacggcgc tgagcgcggg
     2281 tgtcgaccgg gaggcccgct gtggcgtagc gggcgatggt ggtcacgatg acgcccgaga
     2341 tgatcagcgt gacgttgaag gcgggggccg cgctgttggt cgtctgcccg agcgatgaca
     2401 ggttgtcctg ccaccatttc gggtcgctcg aggtgagcat ggcggcgaac gctccgacca
     2461 ccaggaagac ggcgagatcg agggagagag tcatgggcgt gaggcgcgcg gcgccgagga
     2521 aggacacgta ggcggtcagc gcgaacgcca cggcgacgag gatcgcaccg gggaaggcga
     2581 acaccggagc gccgatgaag ctcagctcca gcagctgggc gatgcccaac caccccagga
     2641 aggcgattga cgcgtaggcc ccgccgatcg cgacgaggtc gaaccagcgc aaccggtcgg
     2701 cggggccgac gaagccgtca cgaggctgat cggccggcgc gtcggagggc cgcacggcca
     2761 gccgcccggc cgtgaaggcg agcaccgcga cgatcgcacc gccgaccgcc gcgtaccatc
     2821 cgatcgaccc agggccggtg atggcgaggt gcgcaccacc gaagatcagc caggagacgg
     2881 ggaacccgat cacgaacgcg gccgcgccgg ccagaagggc gacggtctcg cgcgcctccg
     2941 ctgttcccag cggacgtcgg agcacgtcct gcatctctgc ggcgatctgc ttcagctgcg
     3001 gcatccgatt ccggccggct cccggccgcc tcccgcgctc actctatggc acccgccgtg
     3061 cgtccttcac gggcacggcg gatagcctgg gcgcatgact cacagcatcc gcgcccgggc
     3121 gatcctcctc gacatggacg gcacgctcgt cgactccacc gccgtcgtgg agaggctgtg
     3181 gctgcggtgg tcccaggcgc acggactgga cccgcgacgc gtgctcgagg tgatccacgg
     3241 gcgccagggg caccagagca tggcgatcct gctccccgac cgcgaccatg cgatcaacct
     3301 cgccgagaac gccgagatgc tcgcgaccga gacggccgat gtcgagggcg tcgtggagat
     3361 ccccggcgcg gccgggctgt tggtcgctct gcgcgacgct ccgcacgcga tcgtgacctc
     3421 ggcggacgtg gcgctgatga ctgcgcggat gcacgctgcc gggctgaccg cgccggcgat
     3481 gaccgtgacg gccgagagcg tgtcagcatc caagcccgac ccggagggat tcctgctggg
     3541 cgcgcggatg ctgggcatcg agccggccga ctgcgtggtg ttcgaggact ccgaagcggg
     3601 cgtgcaggcc gggctcgcgg ccggcatgcg cgtgatcggc gtcggcgccc acgcgcatgc
     3661 gcacggcgcg accttcgtcg tcgacgacct cacgcacgtg caggtgcgcg cggacgagga
     3721 cgggatcgag ctcactctca gctgagccgg tcaggcggcg ctcggcgtct cgtaggcccg
     3781 ccgaagggtc agtcgcggcc gaagtcctcg aagatcagcg tggtgcgggt cgagcggatg
     3841 aacgggatcg cctggatgtc ctccaggacg atgcgccgca gatcacgtgc gtcactggcc
     3901 cgcacgagca ggatcacgtc gaagtcgcct ccgacgagtg ccatgtgctc gatctcccgg
     3961 atcgcccgca gtcgcgcctg cacgtcctgc cagctggact gctcgatggc gagcatgacg
     4021 taggccgatg cgtgatggcc gagctgcacc ggatcggtgc gcaccgtgta gccggtgatc
     4081 accccggcat ccgtcagccg cttgatgcgg gcgtgcgcac cggcgcgcga gatgtgcacg
     4141 gcgtcggcga tggctgtcat cgaggtgcgc gcatcccggc gcagctcatc caggatcgcc
     4201 cgatccacgg catccagatc cgacatcggc gcccctttcg ttccgtcagc agatcctgac
     4261 acttcgcacg tattcagccg atcgtactgt cgaacgtcca cttttgtgcc actcctgtcg
     4321 aacgattgat gattccgcgg cacactgtgc gaagaggttc cgcaaggagg cgaacacgat
     4381 gcagcacgaa gacctgctcc cccgcgacac tcccgtcgca ctcatcgaca aggacggcgc
     4441 gtccgtcgcc gacgagcagt acgcgctgcc ggatgccgag acgctgcagc gcgcgtaccg
     4501 cggcctcgtc gagggccgtc gcatcaacga ccaggcgtac gcgctcgtgc ggcagggacg
     4561 tctcgccgtg tacccctcct cgcacggcca ggaggcgtgc caggtcgccg cgtccatggt
     4621 gctcggcgag catgactggc tgttccccac ctaccgcgac tccgtcgccg tgatctcccg
     4681 gggcgtgcgc ccggcagacg ccatggtgct gctgaagggc gactggcact ccggctacga
     4741 cccggccgag catcgcgtcg ccccgcagtc cacgccgctg gccacccagc tgctgcacgc
     4801 cgtcggcttc gcacaggccg ccaagcatcg cggcgaggac accgtggtgc tcgccctgtg
     4861 cggcgacggc tccaccagcg agggcgactt ccacgaggcg ctcaacttcg cggccgtgtt
     4921 ccacgtgccg gtcgtgttct tcgtgcagaa caacgagttc gcgatctccg tccccctctc
     4981 ccgccagacc gcagcaccct cgctcgcgca caaggcgatc ggctacggca tgccggggca
     5041 gcgcgtggac ggcaacgacg tcgcagccct cctcgccgtg ctcggcgagg ccgtcgaccg
     5101 cgcacgcagc ggtggcggcc cctcgctggt cgaggcgcac acctatcgca tgcaagccca
     5161 caccaacgcc gacgacgaca cccgctaccg cgaccgcgac gaggtgaagg cgtgggcgga
     5221 ccgcgacccg ctcagccgta tgcaggtctt cctgcgggcc cagggcctgc tcgacgacgc
     5281 cgcgcagcag ggcatcgccg acggcgccga ggtgatcgcc gcagcgctgc gcgatgcgct
     5341 gaacgccgat tccgacctcg accccgagga cctgttccgc ttcgtcaccg ccgagcgctc
     5401 ccctcagctc gaggagcagt ggcaggtgct gcgcggcgag atcgaacgcg catccctcac
     5461 cgacgctgga ggccaccgat gaccatggcg aacgacatcg agatcgagac cccgcacgtg
     5521 gtcggcacca tgaccatggc cgcggcgatc aaccgcgcgc tggccgacgc catcgccgag
     5581 gacccggacg tcgtcgtctt cggtgaggac gtgggcgcgc tcggcggcgt gttccgcatc
     5641 accgacgggc tggccgagcg cttcggcgag gaccgctgct tcgacacccc gctggccgag
     5701 tcgggaatca tgggcaccgc ggtcggcatg gccatgaacg gcatgcgccc cgtcgtcgag
     5761 atgcagttcg acgcgttcgc gctgcccgcc ttcgagcagg tggtcagtca tgtcgcgaag
     5821 atgggcaacc gcacgcgagg tgccgtgcgg atgccgatcg tgatccgcat cccgttcggc
     5881 ggcggcatcg gcggtgtgga gcaccactgc gattcctccg aggcgtacta cgcgcacacc
     5941 cccggcctca ccgtggtctc gccgtccacg ccgcaggacg cgtactcgct gctgcgtgcg
     6001 gcgatcgcct cccccgaccc ggtcgtgttc ctggagccga agaagctgta ctggtcgaag
     6061 ggcgaggtcg acacctccgt cacggccgac ctcggcaccg cgcgcatcgc ccgtgagggc
     6121 acggatgtca ccgtcctggc gtactcggcg atggtgcccg tcgcactcga ggccgccgag
     6181 atcgcggcat ccgaagggcg cagcgtgcag gtcatcgacg tgcgcagcct ctcccccttc
     6241 gacgacgcga cagtgaccgc cgccgtgcgc tcgaccggtc gtgccgtcgt cgtggccgag
     6301 gcgccggggt acgtcagcgt tgcgagcgag atccaggcgc gggtgttcga gcgctgcttc
     6361 gagtacctcg aggcgccggt gcgtcgcgtc accggtttcg acaccccctt cgcgccgccg
     6421 aagctcgagc actggtatct gccggatgcc gatcgtgtcc tggacgccat cgactcgctg
     6481 cactgggatg acgacgcgga aggcgcagca cgatgagcac cacgaccgcg cgggtgttcc
     6541 gcctgccaga tctgggcgag ggcctcaccg aggccggcct cgtgcagtgg ctcgtgaagg
     6601 tgggcgacac catcgccacc gaccaggcca tcgccgaggt cgagacagcg aagagcatcg
     6661 tcgagcttcc ctccccgttc gcgggcgtca tcacagcgct gcacggcgaa cccggcgatg
     6721 tcatcgacgt cggcgccccg gtgctcgagg tcgcctcgga tgcccctgag gcgccgacgc
     6781 ccaccgcatc cgacgccacc gagaaggacg cctatcgcgc cgaggagcgc gccggctccg
     6841 ggaacgtcct gatcggctac gggaccaccg agcgcagcgg ctccggccgt cgccgccctc
     6901 gcgcagtatc cgc
//
LOCUS       DATMSX010000222         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_135758,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000222 DATMSX010000000
VERSION     DATMSX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135758"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VN041_06875"
     rRNA            <1..330
                     /locus_tag="VN041_06875"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            516..592
                     /locus_tag="VN041_06880"
     tRNA            516..592
                     /locus_tag="VN041_06880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:550..552,aa:Ile,seq:gat)
     gene            751..826
                     /locus_tag="VN041_06885"
     tRNA            751..826
                     /locus_tag="VN041_06885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Ala,seq:tgc)
     gene            1231..>2438
                     /locus_tag="VN041_06890"
     rRNA            1231..>2438
                     /locus_tag="VN041_06890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          689 a          520 c          706 g          523 t
ORIGIN      
        1 ataggtgggg ctacacacgt catacaatgg ctggtacaaa gggttgccaa cccgcgaggg
       61 ggagctaatc ccataaaacc agtcgtagtc cggatcgcag tctgcaactc gactgcgtga
      121 agtcggaatc gctagtaatc gcggatcagc atgccgcggt gaatacgttc ccgggtcttg
      181 tacacaccgc ccgtcacacc atgggagcgg gtctcgccag aagtaggtag cctaaccgta
      241 aggagggcgc ttaccacggc ggggttcgtg actggggtga agtcgtaaca aggtagccgt
      301 atcggaaggt gcggctggat cacctccttt ctggaaaaca gcttcgctgt ttcggtggat
      361 gctaactttg tgcaaacaaa gttaagtgcc atcggcacgg caggagccaa aaaagcaatc
      421 aagattgaac gcccacactt atcggttgtt ggaacaagcc aaagctttta aaggctgggt
      481 ctgcacaaga cacggcactt gcaaagaatt ggaatgggtc tgtagctcag ctggttagag
      541 cactgtgttg ataacgcagg ggtcgttggt tcgagcccaa ctagacccac caaatacttc
      601 caacggattt agaggttgag cttatgccgc tttggttgat tggctaaatg ctgattgact
      661 agagccaagc agtgcgaggc gtctggtgcg aagcacacta gcgtgtgtga gcagcaggca
      721 acgaagcaat gcgcaggcta taggcaatca gggggattag ctcagctggg agagcacctg
      781 ctttgcaagc agggggtcgt cggttcgatc ccgtcatcct ccaccaataa cctggttgtc
      841 atgccaggct ccgatagaca cttatcactc aacaccaaag cggtttcgca agagactgtt
      901 ttgttgttga ttccggattc ccggatcaaa cggctgttct ttaaaaattc atagagtcga
      961 aaatcagcgt tgctggtgga aactgcacat tcgtaaaggt ttagtgcaga ccgtgccacc
     1021 agcaacaatt tgattgcgtc aaaacgaatg aaactttgcg agagcaattt tattcagtaa
     1081 tgacgaatat tcttcaagaa tgtggatccg ggagaccggg tctacagcaa agatattcac
     1141 attacggcat aacgcgcgag gtgagagacc tcgcaaagca gttgcttggg ttcttggctt
     1201 gagatctaga gatagatgtc aaagttatag ggtcaagtga ctaagagcat gtggtggatg
     1261 ccttggcgat gataggcgac gaaagacgtg atagcctgcg ataagcttcg gggagttggc
     1321 aaataaactt tgatccggag atttctgaat gggggaaccc acctagcaat aggtatcact
     1381 gagtgaatac atagctcagt gaagcgaacc tggagaactg aaacatctaa gtacccagag
     1441 gaaaagacat caaccgagat tccgatagta gtggcgagcg aattcggaag agccttgcag
     1501 tgatagtcag tgtgttaaca aaacggaatg gaaagtccgg ccatagtggg tgatagcccc
     1561 gtatgtgaaa acgcattggt ggtactaggc tgcagacaag tagggcgggg cacgagaaac
     1621 cctgtctgaa tatgggggga ccatcctcca aggctaaata ctcatcatcg accgatagtg
     1681 aacaagtacc gtgagggaaa ggcgaaaaga accccgggag gggagtgaaa tagatcctga
     1741 aaccgcatgc ttacaaaaag tcggagcctt taggggtgac ggcgtacctt ttgtataatg
     1801 ggtcagcgac ttacattcag tggcaagctt aaccgaatag ggaaggcgta gagaaatcga
     1861 gtccgaatag ggcgacaagt cgctgggtgt agacccgaaa ccaagtgatc tatccatggc
     1921 caggatgaag gtgccgtaac aggtactgga ggtccgaacc gactagtgtt gcaaaactag
     1981 cggatgagct gtggataggg gtgaaaggct aaacaaactt ggaaatagct ggttctctcc
     2041 gaaaactatt taggtagtgc ctcaagtatt accatcgggg gtagagcact gttttggcta
     2101 gggggtcatg gcgacttacc aaaccaaggc aaactccgaa taccgatgag tacagcttgg
     2161 gagacagagc accgggtgct aacgtccgga ctcaagaggg aaacaaccca gaccgccagc
     2221 taaggtccct aaaattggct aagtgggaaa cgaagtggga aggctaaaac agtcaggatg
     2281 ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactgatc gagtcgtcct
     2341 gcgcggaaga tgtaacgggg ctaagccagt taccgaagct gcggatttgc aatttattgc
     2401 aagtggtagg agagcgttct gtaagcctgt gaaggtgc
//
LOCUS       DATMSX010000223         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_12698,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000223 DATMSX010000000
VERSION     DATMSX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12698"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="acnA"
                     /locus_tag="VN041_06895"
     CDS             <1..710
                     /gene="acnA"
                     /locus_tag="VN041_06895"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077426.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWU28804.1"
                     /translation="AKLGNSVTTDHISPAGSIKADSPAGTYLTEHGIDRKDFNSYGSR
                     RGNHEVMIRGTFANIRLKNQLLDGVEGGFTRDFTQPDGPQAFIYDASQNYQAQGIPLV
                     VLGGKEYGSGSSRDWAAKGTSLLGVRAVITESFERIHRSNLIGMGVVPLQFPAGESWE
                     SLGLDGTEIISIEGLTALNEGITPKTVKVTATPSEFSPEGKQVVEFDAVVRIDTPGEA
                     DYYRNGGILQYVLRSLV"
     gene            894..2837
                     /gene="dxs"
                     /locus_tag="VN041_06900"
     CDS             894..2837
                     /gene="dxs"
                     /locus_tag="VN041_06900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWU28805.1"
                     /translation="MPILPSITGPRDLDRLSPDQLAELAAEIRTFLVEKVARTGGHLG
                     PNLGVVELTIALHRVFSSPEDPIIFDTGHQSYVHKLLTGRQDFSQLRSRGGLAGYPQR
                     SESAHDVVESSHASSSLSWADGVSRALTATGRGDRHVVAVVGDGALTGGMTWEALNNI
                     SDDNDRNLVIVVNDNGRSYAPTIGGMSRYLNRVRTAAAYRDLHRKSDRVFRAFGPFGR
                     ALFRGVRGGTHGFLSRFTNNVALYSNLDIKYLGPVDGHDLPALLETLQLAKDFGAPVI
                     VHVITEKGRGYQPALDDEADQFHSVGRIDPKTGGALGKSSGRSWTSVFADALVDVGGR
                     DEKVIAMTAAMLRPTGLAPFAERYPHRVYDVGIAEQHAVASSAGLAFGGLHPVVAIYA
                     TFMNRAFDQVLMDVALHKAGVTFVLDRAGVTGPDGPSHHGIWDLAMLQLVPGIRIAAP
                     RDAARLQEVLDEAVAVDDAPTVIRYPKGSVPDDIPAVERLADGVDVLSRDETEDVLLV
                     GIGPFTELALDVAARLRAQGIGATVIDPRWAIPVQPSILELAARHRLVITLEDGIRVG
                     GVGTRVRQVLREAGIDTAVDELGVPDAFIEHASREQVLQDAGLTAAKIAQDVVAQVLG
                     KRVPMARQTGETGAIELPIHHRR"
BASE COUNT          441 a         1052 c          961 g          440 t
ORIGIN      
        1 tggccaagct cggcaactcg gtcaccaccg accacatctc gcccgccggc tccatcaagg
       61 cggacagccc ggccggcacg tacctcaccg agcacggcat cgaccgcaag gacttcaact
      121 cctacggctc gcgccgtggc aaccacgagg tgatgatccg cggcacgttc gcgaacatcc
      181 gcctgaagaa ccagctgctc gacggcgtgg agggcggatt cacgcgcgac ttcacgcagc
      241 ccgacggtcc gcaggcgttc atctacgacg cctcgcagaa ctaccaggcg cagggcatcc
      301 cgctcgtcgt cctcggcggc aaggagtacg gctccggctc gtcgcgcgac tgggcggcca
      361 agggcaccag cctcctcggc gtccgggcgg tcatcaccga gagcttcgag cgcatccacc
      421 gctccaacct catcggcatg ggcgtcgtcc cgctgcagtt ccccgcgggc gagtcctggg
      481 agtcgctggg cctggacggc accgagatca tctcgatcga gggcctgacc gcgctcaacg
      541 agggcatcac gccgaagacg gtgaaggtca ctgcgacccc gagcgagttc tcgcccgagg
      601 gcaagcaggt cgtcgagttc gatgccgtgg tccgcatcga cacccccggt gaggctgact
      661 actaccgcaa cggcggcatc ctgcagtacg tgctgcgttc gctggtctga tcccacgacg
      721 agagcgcccc acccggcttc gaccgggcgg ggcgctctcg cgtccccgag gagtgcagcg
      781 ccgaaccccg gtcgtccgcg ccacgcaccg aactcccacc gtctcggaga tagactggac
      841 aggttcgcgc gagccctccg gccgcgcagt cgtcgagtcg cggaggagcg tccatgccga
      901 tcctgccgag catcaccggt ccccgggatc tcgaccggct ctcacccgat cagctcgctg
      961 agctggcggc cgagatccgc accttcctgg tcgagaaggt ggcccgcacc ggtggtcacc
     1021 tcggtccgaa cctcggcgtc gtcgagctga cgatcgccct gcaccgggtg ttctcctcgc
     1081 ccgaggaccc gatcatcttc gacaccgggc accagtccta cgtgcacaag ctgctgaccg
     1141 gcagacagga cttctcgcag ctgcgctccc gcggcggtct cgccggctac ccgcagcgct
     1201 cggagagcgc gcacgacgtg gtggagtcct cgcacgcctc cagctcgctg agctgggccg
     1261 acggcgtctc ccgcgcactc acggccacag gacgcggcga ccgtcatgtg gtcgcggtcg
     1321 tcggcgacgg cgcgctcacg ggtggtatga cgtgggaggc tctgaacaac atctccgacg
     1381 acaacgatcg caatctcgtg atcgtcgtca acgacaacgg acgctcctac gcgcccacca
     1441 tcggcggcat gtcccgttac ctcaaccggg tgcgcaccgc cgccgcctat cgcgacctgc
     1501 accgcaagtc cgaccgggtg ttccgcgcct tcggcccgtt cgggcgggcg ctgttccgcg
     1561 gagtgcgcgg tggcacgcat ggcttcctct cccgcttcac gaacaacgtc gcactgtatt
     1621 ccaatctgga catcaagtac ctcggtccgg tcgacggtca cgacctgccc gctctgctgg
     1681 agacgctcca gctggcgaag gacttcggtg cccccgtgat cgtgcacgtc atcaccgaga
     1741 agggccgcgg ttaccagccg gccctcgacg acgaggcgga ccagttccat tccgtcggac
     1801 gcatcgatcc gaagacggga ggggcgctgg ggaagtcctc gggccgctcg tggacctcgg
     1861 tgttcgccga cgccctggtc gacgtcggcg gcagggacga gaaggtcatc gcgatgaccg
     1921 ccgccatgct gcgaccgaca ggcctcgcgc ctttcgctga gcgctatccg caccgcgtgt
     1981 acgacgtggg tatcgccgag cagcacgccg tcgcctcgtc ggccggcctg gcgttcggtg
     2041 gcctgcaccc cgtggtggcg atctacgcga ccttcatgaa ccgcgccttc gaccaggtgc
     2101 tgatggatgt cgcgctgcac aaggcgggcg tcacgttcgt gctggaccgg gccggtgtca
     2161 ccgggcctga cggcccgagc caccacggga tctgggatct ggcgatgctg cagctggtcc
     2221 ccggcatccg catcgcggcc cctcgggacg cggcgcgtct gcaggaggtc ctcgacgagg
     2281 ccgtggccgt cgacgacgcg cccacggtga tccgctaccc gaaggggtcg gtaccggacg
     2341 acatccccgc cgtcgaacga ctggcggacg gggtcgacgt gctctcccgc gacgagaccg
     2401 aggacgtgct cctggtgggg atcgggccct tcaccgagct cgccctcgac gtcgccgctc
     2461 gcctgcgcgc gcaggggatc ggcgccaccg tgatcgaccc gcgctgggcg atccccgtgc
     2521 agccgtcgat cctggaactg gccgcgcgcc accgcctggt gatcacgctc gaggacggca
     2581 tccgcgtggg cggcgtcggc actcgggtgc gtcaggtgct gcgtgaggcg ggcatcgaca
     2641 ccgccgtcga cgagctcggc gtgccggacg cgttcatcga gcacgcgtcg cgcgagcagg
     2701 tgctgcagga tgccggtctc acggccgcga agatcgcgca ggacgtcgtc gcccaggtgc
     2761 tcggcaagcg cgtcccgatg gcgcgtcaga ccggcgagac cggtgcgata gagctgccga
     2821 tccatcaccg caggtgaggc tcaggcctcc agcgcgtcgt cctccacggc ggcgtcgcga
     2881 tcgatgcgcg cgtg
//
LOCUS       DATMSX010000224         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_117095,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000224 DATMSX010000000
VERSION     DATMSX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117095"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="VN041_06905"
     CDS             <1..2184
                     /locus_tag="VN041_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU28806.1"
                     /translation="EAPVADAVLEWARAEHPEFRPTPEQAAVIGTTHGHALVEARAGS
                     GKTTTMIARAAYLIERRGVQPDEILMLAFNSAAAADLRRRAGEWLPADAQPHIMTLDA
                     LAYRLVRPVERVLADDADRRVEEIVRSLWHDPASQDEMRELVLSFVRADADGETGTRY
                     WSLRGDALPTRAAQSIADALLRSGLGSDGRDFRFHRSSSVFELLDADGGSRLRIAITD
                     PRRPFSADLRYDERTTAASGFEERLRRDLRSAGVPTAEMDDDRLWEAIRGSIGERLLR
                     AITQIVMRARQRMWSGADLLREWRQRDGDESRRGAIELGARMLDLYVRALAEAQEEDF
                     PGIVWRAAARVGEGLTDFSGGGRSGDVRAITHIVIDEFQDFTPRFDALIQAVLGQAPR
                     ARVMAVGDDWQAINEFAGSSTEYFAGFTARFPGAIRLRMLGNQRSSADIVDVGNSIMA
                     GRGRPARAVADLTGRIRQFGMDGFEPTPEEERLGRPSTSAVLRLVKEHLGNGRTVAVL
                     ARTREVAERAVAGLARVVPPDAVARCDIGTVHSYKGREADAVILVGVAGWAFPHVHPT
                     WQLGRVFGDSFASILTAERRLLYVGMSRPRRWLDVVTGREDDPSPLWESASLLPAVHR
                     TDWASVAPALGAPAVRRATVRVYNGMYDGRWLLSWDDFHESKSLLTADGGFRYEPSGG
                     RAHWVARMDAAELHARDVLDHPWARFDGIRVEVEWPDRRRQVFTR"
     gene            2281..4191
                     /gene="typA"
                     /locus_tag="VN041_06910"
     CDS             2281..4191
                     /gene="typA"
                     /locus_tag="VN041_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWU28807.1"
                     /translation="MAHALRSDLRNVAIVAHVDHGKTTLVDAMLRQTGSFGSHEHMEE
                     RAMDSNDLEREKGITILAKNTAITYKGEHTETPVTINVIDTPGHADFGGEVERGLSMV
                     DGVVLLVDASEGPLPQTRFVLRKALEAKLPVILLVNKTDRADARIAEVEEEAHDLLLG
                     LASDLVDDVPDLDVDALLDVPVVYASGRAGAASRNRPANGDLPDNDDLEPLFEAILEH
                     VPAPSYDDEAPLQAWVTNLDSSSFLGRLALLRVFNGTLKKGQTVAWVRQDGTTQNARI
                     TELLMTKALDRYPAESAGPGDIAVIAGFADIMIGETIADPEDVRPLPQITVDEPSISM
                     TIGTNTSPLVGKVKGHKLTARMVKDRLDRELIGNVSIRVNDIGRPDAWEVQGRGELQL
                     AILVENMRREGFELTVGKPQVVTRKIDGKVHEPYEHLTVDVPEEHLGAVTQLMANRRG
                     RMDNMINHGTGWIRMEFIVPSRGLIGFRTEFLSITRGTGIANAISHGYDEWAGSIVAR
                     QNGSIVADRAGVATPFAMIALQERMSFFVRPTEEVYEGMVVGENSRADDMDVNITKEK
                     KLTNMRSSTADTFESMTPPRILSLEESLEFAREDECVEVTPDAVRIRKVILDQTVRGR
                     EASRLKRQDANA"
     gene            4512..>4985
                     /locus_tag="VN041_06915"
     CDS             4512..>4985
                     /locus_tag="VN041_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28808.1"
                     /translation="MSARSAMRKPLVGVAVGAVLAAAAATGILAAPALAAPSQADVHK
                     ASQAESDGRIALNNAVALNHVAESADVLPGTTLKDVAITDLMHDVKVLSSAEQPSSDQ
                     LADMTDDVVRSTVLVQRDTAALQESLTAAKEAKAAEIARQQAEAARIAAEQAAEQQ"
BASE COUNT          791 a         1772 c         1711 g          711 t
ORIGIN      
        1 gaggcaccgg tcgcggacgc cgttctcgag tgggcccgtg cggagcatcc ggaattccgg
       61 cccacgcccg aacaggcggc cgtgatcggc acgacccacg ggcatgcgct cgtcgaggcc
      121 cgcgccggga gcgggaagac cacgaccatg atcgcccgcg ccgcctacct gatcgagcgg
      181 cgcggtgtac agcccgacga gatcctgatg ctcgcgttca acagcgccgc ggccgcggat
      241 ctccggcgtc gcgcggggga gtggctgcct gcggacgctc agccgcacat catgacgctg
      301 gacgcgctcg cctaccgcct ggtccgtccg gtggaacgtg tgctcgccga cgacgcggat
      361 cggcgtgtcg aggagatcgt gcgatccctg tggcacgacc ccgcgtcgca ggacgagatg
      421 cgggaactgg tgctgtcgtt cgtgcgggcg gacgccgacg gcgagaccgg caccaggtac
      481 tggagcctgc gcggcgatgc gctcccgacc cgcgccgcgc agagcatcgc cgacgccctg
      541 ctgcgcagcg ggctgggcag tgatgggcgg gacttccggt tccaccgcag cagctccgtc
      601 ttcgagctgc tcgacgcgga tggcgggtca cggctgcgga tcgccatcac cgatccgcgc
      661 cgcccgttct ccgccgacct gcgctacgac gagcggacca cggcggcgag cgggttcgag
      721 gagcgtctgc gccgcgatct ccgctcggcg ggcgtaccga ccgccgagat ggacgacgac
      781 cgcctgtggg aggcgatccg ggggtcgatc ggcgagagac tgctgcgcgc gatcacgcag
      841 atcgtgatgc gtgcgcggca gcgcatgtgg agcggtgcgg acctgctgcg ggagtggcgg
      901 cagcgcgacg gggacgagtc ccggcgaggg gcgatcgagc tcggcgcccg gatgctcgac
      961 ctctacgtgc gggcgctcgc cgaagctcag gaagaggact tccccgggat cgtctggcgc
     1021 gcggcggcgc gggtcgggga gggtctgacc gacttctccg gcggtggccg cagcggagac
     1081 gtccgcgcga tcacgcacat cgtcatcgac gagttccagg acttcacacc gcgcttcgac
     1141 gccctgatcc aggctgtgct cggccaggcg cctcgggcga gggtcatggc cgtcggcgac
     1201 gactggcagg ccatcaacga gttcgccggg tcgtcgaccg agtacttcgc agggttcacc
     1261 gcccgattcc caggagcgat ccgactgcgg atgctgggga atcagcgctc cagcgccgac
     1321 atcgtcgacg tcggcaacag catcatggcc gggcgcggca gaccggccag ggcggtggca
     1381 gacctcaccg gccgcatcag gcagttcggg atggacgggt tcgagccgac gcccgaagag
     1441 gagcggctcg gccgtccgtc gacgtctgcc gtgctgcggc tggtcaagga gcacctcgga
     1501 aacgggcgca cggtggccgt gctggcgcga acgcgcgagg tcgcggagcg cgccgtcgcg
     1561 ggccttgcgc gggtcgtgcc gccggatgcc gtcgcgcgct gcgacatcgg caccgtccac
     1621 tcctacaagg gccgggaggc ggatgctgtg atcctggtgg gcgtggccgg atgggcgttc
     1681 ccgcacgtgc atccgacctg gcagctcggg cgcgtgttcg gcgactcctt cgcctcgatc
     1741 ctcaccgcgg agcgacgact gctctacgtc gggatgagca ggccccgtcg ctggctcgac
     1801 gtcgtgacgg gtcgcgagga cgacccgtcg ccgctctggg agagcgcctc gctgctgccc
     1861 gccgtgcacc gcacggactg ggcatcggtc gctcccgccc tcggcgcgcc ggccgttcga
     1921 cgtgcgaccg tgcgggtgta caacggcatg tacgacgggc gctggctgct ctcctgggac
     1981 gacttccatg agagcaagag cctgctcacg gccgacggcg gcttccgcta cgagccgtcc
     2041 ggcgggcgtg cgcactgggt cgcgcggatg gatgccgccg agctgcacgc gcgggacgtg
     2101 ctcgatcacc cgtgggcgcg tttcgacggc atccgcgtcg aggtcgagtg gcccgaccgc
     2161 cgtcgtcagg tgttcacccg ctagcctgac cgggccttca ggaaggtgct gatggcgtgc
     2221 gctatcctgg aacgtcggca tcccggcgca caaatcccaa ctccgcttag gactccttga
     2281 atggcgcacg ccctccgctc tgacctccga aacgtcgcga tcgtcgcaca cgtcgaccac
     2341 ggcaagacca ctctcgtcga cgcgatgctc cgtcagacgg gctcgttcgg ctctcacgag
     2401 cacatggaag agcgcgccat ggactcgaac gacctggagc gcgagaaggg catcacgatc
     2461 ctcgccaaga acacggcgat cacctacaag ggcgagcaca ccgagacgcc cgtgacgatc
     2521 aatgtgatcg acacccccgg ccacgccgac ttcggcggcg aggtggagcg cgggctgtcg
     2581 atggtcgacg gcgtcgtgct gctggtggac gcctccgagg gcccgctgcc gcagacacgc
     2641 ttcgtgctgc gcaaggcgct cgaggccaag ctccccgtca tcctgctggt caacaagacc
     2701 gaccgcgccg acgcccgcat cgcggaggtc gaggaggagg cgcacgacct gctgctgggc
     2761 ctggcgtccg acctggtcga cgacgtgccc gacctcgacg tcgacgccct gctggacgtg
     2821 ccggtcgtgt acgcctccgg ccgtgccggt gcggcctccc gcaaccgccc cgccaacggc
     2881 gacctgcccg acaacgacga cctcgagccg ctcttcgagg cgatcctcga gcacgtgccc
     2941 gcaccgtcct acgacgacga ggccccgctg caggcgtggg tcaccaacct cgactcctcg
     3001 tccttcctcg gccgcctcgc gctgctgcgc gtcttcaacg gcacgctgaa gaaggggcag
     3061 accgtcgcct gggtgcgtca ggacggcacc acccagaacg cgcgcatcac cgagctgctg
     3121 atgaccaagg cgctggaccg ctacccggcc gagtccgccg gccccggcga catcgccgtg
     3181 atcgcgggct tcgccgacat catgatcggt gagaccatcg ccgaccccga ggacgtgcgc
     3241 ccgctgccgc agatcacggt cgacgagccg tcgatctcga tgacgatcgg caccaacacc
     3301 tcgccgctgg tcggcaaggt caagggtcac aagctcaccg cccgcatggt gaaggaccgc
     3361 ctggaccgcg agctcatcgg taacgtctcg atccgcgtga acgacatcgg ccgtccggat
     3421 gcctgggagg tgcagggccg cggtgagctg cagctcgcga tcctggtcga gaacatgcgc
     3481 cgcgagggct tcgagctcac ggtcggcaag ccgcaggtcg tcacccgcaa gatcgacggc
     3541 aaggtgcacg agccctacga gcacctcacg gtcgacgtcc cggaggagca cctcggtgcc
     3601 gtgacccagc tgatggccaa ccgccgtggc cgcatggaca acatgatcaa ccacggcacc
     3661 ggctggatcc gcatggagtt catcgtgccc tcgcgcggcc tgatcggctt ccgcaccgag
     3721 ttcctctcga tcacccgcgg caccggtatc gcgaacgcga tctcgcacgg ctacgacgag
     3781 tgggccggct ccatcgtggc ccgtcagaac ggctcgatcg tcgcggaccg cgccggcgtg
     3841 gccaccccgt tcgccatgat cgccctgcag gagcgcatgt cgttcttcgt gcgtcccacc
     3901 gaagaggtct acgagggcat ggtcgtgggc gagaactcgc gcgccgacga catggacgtg
     3961 aacatcacca aggagaagaa gctcaccaac atgcgctcct ccacggccga cacgttcgag
     4021 tcgatgaccc cgccgcgcat cctgtcgctg gaggagtccc tcgagttcgc ccgcgaggac
     4081 gagtgcgtcg aggtcacccc ggatgccgtc cgcatccgca aggtgatcct ggaccagacc
     4141 gtccgcggtc gtgaggcatc gcgcctgaag cgtcaggacg ccaacgcctg acgcgcgttc
     4201 cgatccaccc gaaggccccg gttccgaccg gggccttcgc ttttcgcggc gggccgtcga
     4261 cggcgaagta acgaacgcat aacacatctt cttgggagcg tgaagccggg attcccacga
     4321 accaggcacg gaatgtccag gtccgtggga tgttccgtcc agaacggggg cgcagactcg
     4381 ttcttcgtgc ccgggaaacc gctcccgatg agcaagccct ccgcgggttc cgggccgacg
     4441 acgacccgaa agtcgaaacc cgaatgcctc ataccctcca cacccgtgcg tcccgccggg
     4501 tgcatgccgc cgtgtccgcc cgctccgcga tgcgcaagcc gctggtcggc gtcgccgtcg
     4561 gcgccgtcct cgccgcagcc gcggccaccg gcatccttgc cgctcccgct ctcgccgccc
     4621 cctcgcaggc tgatgtccac aaggcatcgc aggcggagag cgacggccgg atcgccctga
     4681 acaacgccgt cgcactgaac cacgtggccg agtcggcgga tgtgctgccc gggaccacgc
     4741 tcaaagatgt cgcgatcacc gacctgatgc acgatgtgaa ggtgctgtcg agcgccgagc
     4801 agccgagctc cgatcagctc gccgacatga ccgatgacgt ggtccggagc accgtgctcg
     4861 tgcagcggga caccgccgcg ctgcaggagt cgctgaccgc cgccaaggag gccaaggccg
     4921 cggagatcgc ccgccagcag gccgaagccg cgcgcatcgc tgccgagcag gccgccgagc
     4981 agcag
//
LOCUS       DATMSX010000225         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3570,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000225 DATMSX010000000
VERSION     DATMSX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VN041_06920"
     CDS             <1..849
                     /locus_tag="VN041_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28809.1"
                     /translation="LSGVLVALTSPGGIIPLAGYLFAFSVLGGIVVATVLLVLRRPLV
                     GIVVAGLIAAVVAVAVLGFHAPDLFASVAVAFSAAIPAMLVSLAHIGAATAIVLWTML
                     DAGASRNGFAAWVRRHSVAITVAAALCAAPYVIARATWLTPWPLFAPSPEVLDADPST
                     RLLGLVLGLGMLTGAVLTLGLVRPWGERFPRWMAGLGGRRVPVGLAVVPASLVAVLFT
                     FGGIDFLVMAATGPKVVAGVLEMALMLPFWLWGPLLALATWGYAMHRRGERSQAPHPA
                     EVVAAG"
     gene            1045..2760
                     /locus_tag="VN041_06925"
     CDS             1045..2760
                     /locus_tag="VN041_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWU28810.1"
                     /translation="MASDYTPPVDDYRFLFGEAFGLDLVARGTGGAFSAEDATEIIAG
                     AGEFAASVLAPLETVGDREGARLADGQVHLPAGFAEAYQAFVEAGWVSAEAPESAGGD
                     GLPGSIRAGLGEIWNGSNAAFALCWLLTAGQIHALDAAASDELRETYLTKLVAGTWTG
                     TMNLTEPDAGTDLGAIRTMATPRADGSWGVSGQKIFITWGDHDVAENIVHLVLARTPD
                     APAGAKGLSLFVVPKFLPDAAGDPGERNAVTTVAVEHKLGIHGSPTCVLAYEDATGYL
                     VGEVGGGLAGMFVMMNSARAGMGFQATGISDRAYQQAVAYADSRLQGAVLDRPAGTPI
                     AGHPDVRRLLLSMQSRLFAMRALGVYLGDLFDRADADGTGALAEFFVPILKGWATEDA
                     VALTSDAIQVHGGMGFIEETGVAQHYRDARIMPIYEGTTAIQSNDLIGRKVIRDGGAT
                     AEQLFAAILASVSDLRALENPVAARTADRLSRAVDAARAATASVVGFGQTRDAYGVSV
                     PYLMLLGTLAGGWMHGLAVVAVLAHASVDEADAARLVSADFYGAHHLPRVHMLAETVA
                     AGEVA"
     gene            complement(2761..4095)
                     /locus_tag="VN041_06930"
     CDS             complement(2761..4095)
                     /locus_tag="VN041_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWU28811.1"
                     /translation="MASPASAPPSLNREILHLAVPALGALVAEPLFLILDAAMVGHLG
                     TTPLAGLGIASAVLQTIVGLMIFLAYSTTPAVARRFGAGRPGDAVSVGIDGMWLALGL
                     GAVLAIAGALTTPWLVSLFGAADTVAVEARTYLTISMWGLPAMLIVYAATGLLRGMQD
                     TVTPLWIAGLGFGANALLNWIFIYGFGWGIAGSAFGTVTAQWGMVAAYVLVIRRLAIR
                     HGASMKAQSEGLRGTARSGGWLLLRNVSMRVALLATVAIATGIGTLELAGWQIAFTIF
                     TTAAFALDALAIAAQALIGKSLGSGDLTQVHRVLRRTTAWGVWFGVLVGALVAALSGV
                     LGIVFTGDAAIAALVQPALIVLAVAQPIAGVVFVLDGVLMGANDARYLAIAGGLNLVP
                     FLPALWIIAATGIDGDVGLLWLAIAFFGVYLVTRLGTLGWRVRTDQWTRAAA"
     gene            complement(4413..>5415)
                     /locus_tag="VN041_06935"
     CDS             complement(4413..>5415)
                     /locus_tag="VN041_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HWU28812.1"
                     /translation="LSLDDVRRLVKRAEILLEQDRLDAREEERRASRSLTFFERDGML
                     HLSLVTDVESGAPIRTAIDGYVTAQFQARKDAEAAAVAARRADGGDGDAGADGAREAG
                     AAHPVASKADGSGVDEDRRAVAMMRTDALTLFCGHVIGCETSVPVAGATVIVRVDLDD
                     LESGTGHGIIDGTDQPVSVAAIRRMAAAGGVIPCVLGSEGEILDLGRERRLFTRAQKL
                     ALTERDGGCARCGLPPHMTKVHHLRWWKRDTGPTDLSNGVLLCESCHHRIHDNGWDIR
                     IDNPPGNHADTSDTTGTRRRQGTGVNVHAKVWFIPPTHIDPTRTPQLGGRARYDIAA"
BASE COUNT          732 a         1796 c         2033 g          854 t
ORIGIN      
        1 ctgtcgggcg tgctggtcgc gctcacctca ccgggcggga tcatcccgct cgccggctac
       61 ctgttcgcgt tctcggtgct cggcggcatc gtcgtggcga ctgtgctgct ggtgctgcgt
      121 cgcccgctgg tcggcatcgt cgtcgccggc ctcatcgccg ccgtcgtggc ggtggccgtg
      181 ctggggttcc acgcccccga cctgttcgcc tcggtcgcgg tcgcattctc agcggccatc
      241 ccggccatgc tcgtgtccct cgcgcacatc ggcgccgcca cggcgatcgt gctgtggacg
      301 atgctcgacg ccggcgcctc gcgcaacggg ttcgccgcgt gggtgcggcg tcacagcgtc
      361 gcgatcaccg tcgcggcggc gctgtgcgcc gcgccgtacg tgatcgcccg tgccacctgg
      421 ctcacgccct ggccgctgtt cgcccccagc cccgaagtgc tcgacgccga cccgtcgacg
      481 cggctgctgg ggctggtgct cggcctcggg atgctgaccg gcgccgtgct cacgctcggt
      541 ctggtgcggc cgtggggcga gcggttccct cgctggatgg cgggtctggg cggacgcagg
      601 gttcccgtcg ggctggcggt cgtgccggcg agcctggtcg cggtgctgtt cacgttcggc
      661 gggatcgact tcctggtgat ggccgcgacg ggtccgaagg tggtcgccgg ggtgctggag
      721 atggcgctca tgctcccgtt ctggctctgg gggccgctgc tcgcgctcgc gacgtggggc
      781 tacgcgatgc atcgccgggg cgagcggtcg caggcaccgc accctgccga ggtggtcgct
      841 gccggctgaa tcaccgcggc gcatgccgaa gtcatcagcg cgtcgacgtc tgaacgagac
      901 gatcgacgcg ctggtgcatg cccgggacgg cgatgtgctt cgcgctgcgg acgggtccac
      961 gggagccgga ggagaacacc tgattccctt gagacgcagt ctcgcgtaaa ctgacgctca
     1021 gtctcaaacc gattggagtc cgccatggca tccgactaca cgccgccggt cgacgattac
     1081 cgcttcctgt tcggcgaggc gttcggcctc gacctggtcg cgcgcggcac gggaggcgcg
     1141 ttcagcgccg aggacgccac cgagatcatc gcgggtgcgg gcgagttcgc tgcatccgtc
     1201 ctggctccgc tcgagaccgt cggcgaccgc gagggcgcgc gcctcgcaga cggccaggtg
     1261 catcttccgg ccgggttcgc tgaggcgtat caggcgttcg tcgaggcggg gtgggtcagc
     1321 gccgaggcgc ccgagtccgc aggcggagac gggctgcccg gcagcatccg cgccggtctc
     1381 ggcgagatct ggaacggctc caacgccgcg ttcgcgctgt gctggctgct gacggcgggc
     1441 cagatccacg cgctggatgc cgcggcatcc gacgagctgc gcgagacgta cctgaccaag
     1501 ctcgtcgccg gcacctggac cggcacgatg aatctcacgg agcccgacgc cggaaccgat
     1561 ctgggcgcga tccgcacgat ggcgacgccg cgcgcggacg gctcgtgggg tgtcagcggt
     1621 cagaagatct tcatcacctg gggcgaccac gatgtggccg agaacatcgt gcacctcgtg
     1681 ctcgcgcgca cccctgatgc tcccgctggg gccaaggggc tgtcgctgtt cgtggtgccc
     1741 aagttcctgc ccgacgctgc cggcgacccg ggcgagcgca acgcagtcac gacggtggcc
     1801 gtcgagcaca agctcggcat ccacggcagc ccgacgtgcg tgctcgccta cgaggacgcc
     1861 accgggtatc tggtcggcga ggtcggcggt gggctcgcgg gcatgttcgt gatgatgaac
     1921 tccgcgcgcg cggggatggg cttccaggcc accggcatct cggaccgcgc ctatcagcag
     1981 gctgtcgcct acgcggattc gcgtctgcag ggggcggtgc tcgaccgccc cgcaggcacg
     2041 ccgatcgccg ggcatccgga cgtgcgccgc ctgctgctgt cgatgcagag ccgtctgttc
     2101 gcgatgcgtg cgctgggcgt gtacctgggt gacctgttcg accgggctga cgccgacggg
     2161 accggcgcgc tcgccgagtt cttcgtgccg atcctgaagg gctgggcgac ggaggatgcc
     2221 gtggcgctga ccagcgacgc gatccaggtg cacggtggca tgggcttcat cgaggagacc
     2281 ggcgtcgcgc agcactaccg cgacgcgcgc atcatgccga tctatgaggg caccacggcg
     2341 atccagtcca acgacctgat cggccgcaag gtgatccgcg acggcggcgc gacggcggag
     2401 cagctgttcg ccgcgatctt ggcgtcggtg tccgacctgc gcgctctcga gaacccggtg
     2461 gctgcgcgca ccgccgatcg cttgtcacgg gctgtcgatg cagcacgtgc ggcgacggca
     2521 tccgtggtcg gcttcggcca gacccgcgac gcctacggcg tgagcgtgcc gtacctgatg
     2581 ctgctcggca cgctcgccgg tggctggatg catgggctcg cggtggtcgc cgtgctcgcg
     2641 cacgcttctg tcgatgaggc ggatgcggct cgtctggtct ccgcggactt ctacggcgcc
     2701 caccacctgc cgcgcgtgca catgctggcg gagaccgtgg cggcgggcga ggtcgcctga
     2761 ctaagcggcc gccctcgtcc actggtcggt gcggacgcgc cagccgaggg tgccgaggcg
     2821 ggtgaccagg tagacgccga agaatgcgat ggccagccac agcaagccga cgtcgccgtc
     2881 gatgccggtg gcggcgatga tccacagcgc gggcaggaac ggcaccaggt tcagcccgcc
     2941 ggcgatggcg aggtagcggg cgtcgttcgc gcccatcagc accccgtcca gcacgaagac
     3001 gacgcccgcg atgggctggg cgacggcgag cacgatcagc gccggctgaa ccagggcggc
     3061 gatcgcagca tccccggtga agacgatccc cagcactccg gagagcgctg cgacgagcgc
     3121 gccgacgagg acgccgaacc acacgcccca ggcggtggtg cggcgcagca cgcggtgcac
     3181 ctgagtgagg tcgccggagc cgagactctt gccgatcagc gcctgcgcgg cgatcgcgag
     3241 cgcgtcgagt gcgaaggccg cggtcgtgaa gatcgtgaac gcgatctgcc agccggcgag
     3301 ctccagcgtg ccgatgccgg ttgcgatggc gacggtggcg agcagggcga cgcgcatgct
     3361 cacgttacgg agaagcaacc atccgccgga ccgggcggtg ccgcggaggc cttcgctctg
     3421 cgccttcatc gaggcgccgt gccggatggc gaggcgacgg atgacgagca cgtaggcggc
     3481 gaccatgccc cactgcgcgg tgacggtgcc gaacgcggag ccggcgatgc cccagccgaa
     3541 gccgtagatg aagatccagt tgagcagggc gttggcgccg aagccgaggc cggcgatcca
     3601 gagcggggtg acggtgtcct gcatgccgcg gagcaggccg gtggcggcgt agacgatgag
     3661 catggcgggc aggccccaca tggagatcgt caggtaggtg cgggcttcga cggcgacggt
     3721 gtcggcggca ccgaacaggc tgaccagcca gggggtcgtg agggcgccgg cgatcgcgag
     3781 gacggcgccg aggccgaggg cgagccacat gccgtcgatg ccgacggaga cggcgtcgcc
     3841 ggggcggccg gcgccgaatc ggcgggcgac ggccggggtg gtcgagtagg cgaggaagat
     3901 catcaggccg acgatggtct gcaggacggc gctggcgatt ccgaggcccg cgagcggggt
     3961 ggtgccgaga tggccgacca tcgccgcgtc gaggatgagg aacagcggct cggcgacgag
     4021 tgctccgagc gccggcaccg cgaggtgcag gatctcgcgg ttcagcgacg ggggagcgga
     4081 tgcgggggat gccacctcac gagcctatgg cgtgcgggcg gagaggtcgt ctccaggcgg
     4141 ggcttagctg cctggtcgct ggtcgctggt cgcgtggtca ggcggtgata tcggcgggcg
     4201 catcggtgct gctgacgtgc accgctcgcc cgtgcatccg cgcgactgtg ggttgccgat
     4261 ccccgtcgcc atgcagccct tcgtcatagt ccggcgcgaa catcggtctg tcgccggggt
     4321 tgtgcagggc cgccgcacgg tgaggtgctc aagccggcga agtgctcaat cggccacgtg
     4381 ccaggcggcc atgcgctcaa gcagccgcgt gctcaggcgg cgatgtcgta gcgggcgcgt
     4441 ccgccgagtt gtggggtgcg ggtggggtcg atgtgggtgg gtgggatgaa ccagactttc
     4501 gcgtgcacgt tgacgccggt cccttgcctt cgccttgtgc cggtggtgtc gctcgtgtcg
     4561 gcgtggtttc cgggtgggtt gtcgattctg atgtcccatc cgttgtcgtg gatgcggtgg
     4621 tggcaggatt cgcagagcag gaccccgttg gacagatcgg tcggcccggt gtcgcgtttc
     4681 caccatctca ggtggtggac cttggtcatg tggggtggga gtccgcatcg ggcgcatccg
     4741 ccgtcgcgtt cggtgagggc gagtttctgg gcccgggtga acaggcgtcg ttcgcgtccg
     4801 aggtcgagga tctcaccctc ggagccgagc acgcagggga tgacgccacc ggcggctgcc
     4861 atccgccgga tggcggccac gctgacgggc tggtcggtcc cgtcgatgat gccgtgtccg
     4921 gtgccgctct cgaggtcgtc gaggtcgacc ctgacgatca cggtcgcgcc tgcgacggga
     4981 acggaggttt cgcagccgat gacgtgcccg cagaacaggg tcagcgcgtc ggtgcgcatc
     5041 atcgcgaccg cgcgccggtc ctcgtccaca ccactgccat ccgcttttga ggccacaggg
     5101 tgggccgcac ctgcctcgcg cgcgccgtca gccccggcat ccccgtcccc gccatcggcc
     5161 cgccgtgcag cgaccgccgc ggcctcggcg tccttccgtg cctggaactg ggcggtgacg
     5221 tatccgtcga tcgcggtgcg gatcggcgca ccggactcca catcggtgac aagactcagg
     5281 tgcagcatcc cgtcgcgttc gaagaaggtc agcgaccggg acgcccgacg ctcctcctca
     5341 cgtgcgtcga gcctgtcctg ctccagcagg atctcggctc gtttgacgag ccggcgcaca
     5401 tcatccaggg acagt
//
LOCUS       DATMSX010000226         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_50710,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000226 DATMSX010000000
VERSION     DATMSX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50710"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VN041_06940"
     CDS             <1..473
                     /locus_tag="VN041_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HWU28813.1"
                     /translation="VQPGASASDDLFETGRRRGGMPWWGWLIAAVGVVAVVLGIVFAS
                     TLAGGLTPTPDKTTAAPPQPKDPIAGLVPNVAELKGTSDAGVVTFTWTNPDPEKGDSY
                     IWYEYTLDGPGTLQTTEKTSVTLDGARTGATCVEVLIKRADGRSSPDPVRGCVG"
     gene            470..1186
                     /locus_tag="VN041_06945"
     CDS             470..1186
                     /locus_tag="VN041_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28814.1"
                     /translation="MMDITVEYAGEYFSLDPDEPFHVGRQGDLELDDNLFLHRNFLEI
                     SNTDGLWWLSNVGARLTATVTDSSGGVNAWLAPGARLPIVFEQTTVVFTAGPTTYEFT
                     IHAKGPAFRIAPREAADGGQSTIGHVPLTESQKLLILALAEPVLRREGTGTSEIPSSA
                     KAAERLGWTSTRFNRKLDNVCDKFDRIGVQGMRGGQGKLATNRRARLVEHAVSSRLVT
                     REDLALLDAPVLVPDDDVDA"
     gene            1274..>2083
                     /locus_tag="VN041_06950"
     CDS             1274..>2083
                     /locus_tag="VN041_06950"
                     /inference="COORDINATES: protein motif:HMM:NF020396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HWU28815.1"
                     /translation="MSPQTTAVAQSQLVRLSVQWESERIDVGVPGGVPVAEVLPSLTR
                     RLRMLDSMSASAGFRLVRADGSALDPDRTLTAQSVHDGDFLVLEQGASAAVPKRYDDL
                     VEAVADVVEENAPAWSVQNSVTTATVVASVLLLLGLVPAVWAWVDSRSIITAAGAGAA
                     AVVLMICALVMDRTGAPTQASMSLALVSSLFAGVAGYTALPGGPGWGLPIVVGSAAML
                     VFGVVTMLALKSRREYGLIPVALGVVFLVIGAIITWLGAPVPATLAAAMAVA"
BASE COUNT          306 a          723 c          738 g          316 t
ORIGIN      
        1 ccgtgcagcc gggcgcctcg gcatccgacg atctcttcga gaccggacgc cgccgcggcg
       61 gcatgccgtg gtggggctgg ctcatcgctg ccgtcggcgt cgtcgccgtg gtgctgggca
      121 tcgtcttcgc cagcacgctg gccgggggac tgacacccac gcccgacaag accacagccg
      181 cgccgccgca gccgaaggac cccatcgcgg gcctcgtgcc gaacgtcgcc gagctgaagg
      241 gcaccagcga tgccggtgtc gtgacgttca cctggaccaa cccggacccc gagaagggcg
      301 actcgtacat ctggtacgag tacaccctgg acgggccggg tactctgcag acgacggaga
      361 agaccagcgt caccctggac ggagcacgta cgggggcgac ctgtgtcgag gtgctgatca
      421 agagggctga cgggcgttcc tcgcccgatc cggtcagggg gtgcgtgggc tgatggacat
      481 cacggtggag tacgcggggg agtacttctc gctcgatccc gacgagccgt tccacgtcgg
      541 caggcagggc gacctcgagc tcgacgacaa tctcttcctg cacaggaact tcctggagat
      601 ctcgaacacc gacgggttgt ggtggctgtc caacgtcggg gcgcgactca ccgcgacggt
      661 gacggactcc tcgggaggag tcaacgcctg gctcgcaccc ggtgcgcggc tgccgatcgt
      721 cttcgagcag accacggtgg tgttcaccgc cgggccgacc acgtacgagt tcacgatcca
      781 cgcgaagggc cccgcgttcc gcatcgcacc tcgcgaggcg gccgacggcg gccagtcgac
      841 gatcgggcac gtgccgctga ccgagagcca gaagctgctg atcctcgcgc tggcggagcc
      901 ggtgctgcgc cgcgagggaa ccggcacgag cgagattccc agctccgcca aggccgcgga
      961 gcgactgggc tggacgagca cgcggttcaa ccgcaagctc gacaacgtct gcgacaagtt
     1021 cgaccgcatc ggcgtccagg gcatgcgcgg cgggcagggc aagctcgcga ccaaccggcg
     1081 ggcgcgtctg gtcgagcacg cggtgtcgag ccggctggtc accagggagg atctggcgct
     1141 gctggacgcc ccggtcctcg tccccgacga cgacgtcgac gcctagtccg acgcaccggt
     1201 aggcaggacg ccgcaaggag cagatcgagc gcgcgatagt gtgaaacgcg cggttcaacg
     1261 aaagcaggga ttcatgtcgc cccagacgac ggctgtcgcg cagtcgcagc tcgtgcgcct
     1321 ctcggtgcag tgggagtccg agcgcatcga tgtgggcgtg ccaggtggcg tcccggtcgc
     1381 ggaggtgctc ccgtcgctga cgcgacgtct gcggatgctg gacagcatgt cggcgtccgc
     1441 cgggttccga ctcgtcaggg ccgacggctc cgccttggat cctgaccgca cgttgaccgc
     1501 gcagagcgtg cacgacggcg acttcctcgt gctcgagcag ggcgcgagcg ccgcagtgcc
     1561 caagcgctat gacgaccttg tcgaggccgt cgccgacgtc gtggaggaga acgcgccggc
     1621 ctggtcggtg cagaactccg tgaccacggc gaccgtggtg gcatccgtgc tgctgctgct
     1681 gggcctcgtt cccgcggtgt gggcctgggt cgactcccgc tcgatcatca ccgccgccgg
     1741 tgccggagcc gccgccgtcg tgctcatgat ctgcgcgctc gtgatggacc gcaccggtgc
     1801 gcccacccag gcgtccatgt ccctcgcgct cgtctcctcg ctgttcgccg gcgtcgcggg
     1861 atacaccgcg ctgccgggcg gcccgggctg ggggctgccg atcgtcgtcg gcagcgccgc
     1921 gatgctcgtc ttcggcgtcg tcaccatgct ggcgctgaag agccgccgcg aatacgggct
     1981 catcccggtc gccctcggcg tcgtgttcct cgtgatcggc gccatcatca cgtggctcgg
     2041 cgcgcccgtc cccgcgaccc tggccgccgc gatggcggtc gcc
//
LOCUS       DATMSX010000227         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3608,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000227 DATMSX010000000
VERSION     DATMSX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VN041_06955"
     CDS             <1..1391
                     /locus_tag="VN041_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28816.1"
                     /translation="VGIPLSLGSHRLPDAFGGLATPRPQPVVRRPLRPKPIIVALTVL
                     ALLLSGGWAASEWPELPAAGRSADFASPDPQAPWLPDYVPDSAQYPKAWASMNAALRA
                     KDRDAFLSYAEGEARDQLALWWDNTERIGRGTAYIVPTTGSDGGAGAFLGAELAFSSR
                     PLRGSGDQDAGYRLTQGFTYDITTKGDGDRMRLTSFTPVTPMPWDEGELYVARGDHVV
                     LYGMADEQALVDATVDTAEQGAVLAIRTITDLGGKVPMAGFVSGITDDQERMDRWQYG
                     EDVPEGDPTVAGYATATNRPAFRSDFFEPDIAVGDATSGVLVMMGPLSTEDRLATFTH
                     EFAHGLHYAAAPLTSYDAPPVAVYEGFATYIELRTGITDIRWLQEPDVKDLVASQGVA
                     ALSDQALTEGEDAWLSYIAAGSYYVFVAENGGDPWKLALDGADAVGKNLIEIENDPRF
                     SEANWKAWLKRR"
     gene            1500..1673
                     /locus_tag="VN041_06960"
     CDS             1500..1673
                     /locus_tag="VN041_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWU28817.1"
                     /translation="MGVMDDAKHNAEKLVGKAKEHIGDATDNEDLEAEGKLDQAKASV
                     KKVGDDVKDAFDK"
     gene            1791..>2007
                     /locus_tag="VN041_06965"
     CDS             1791..>2007
                     /locus_tag="VN041_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWU28818.1"
                     /translation="MPFTGRFGIEEEFALLDAFTLVPVSAEGVHAAVLGDVPGGGRLS
                     AEFMTSQVEAATEPVGSLAQAREQLVRM"
BASE COUNT          323 a          668 c          736 g          280 t
ORIGIN      
        1 ccgtcggcat cccgctctcg ctcggctcgc accggttgcc ggacgccttc ggcggactcg
       61 cgactccgcg accgcagccg gtggtgcgcc gtccgctgcg cccgaagccg atcatcgtcg
      121 cgctcaccgt gctggcgctg ctgctcagcg gcggctgggc ggcatccgag tggccggagc
      181 tgcccgcagc aggccgctcc gccgacttcg cctcgcccga tccgcaggcg ccctggctgc
      241 cggactacgt ccccgactcg gcgcagtacc cgaaggcgtg ggcgtcgatg aacgcggcgc
      301 tgcgcgccaa ggaccgcgac gcgttcctct cctacgccga gggcgaggcc cgggaccagc
      361 tcgcgctgtg gtgggacaac accgagcgga tcgggcgggg gaccgcctac atcgtcccga
      421 cgaccggcag cgacggaggg gcgggcgcgt tcctgggcgc cgagctggcg ttctcgtcgc
      481 gtccgctgag ggggagcggt gatcaggacg cgggctaccg gctgacgcag ggtttcacct
      541 acgacatcac caccaagggc gacggcgaca ggatgcgcct cacgtcgttc acgcccgtca
      601 cgccgatgcc gtgggatgag ggtgagctgt acgtcgcgag aggcgaccac gtcgtgctgt
      661 acggcatggc ggacgagcag gccctcgtcg acgcgacggt cgacaccgcc gagcaggggg
      721 cggtgctggc gatcaggacg atcaccgacc tgggcggcaa ggtgccgatg gcgggattcg
      781 tgtcgggcat caccgacgat caggaacgca tggaccgctg gcagtacggc gaggacgtgc
      841 ccgaggggga cccgacggtc gccggctacg cgaccgccac gaaccggccc gcgttccggt
      901 cggacttctt cgagcccgac atcgccgtgg gcgatgcgac ctccggcgtg ctggtgatga
      961 tgggcccgct gagcaccgag gaccggctgg cgaccttcac gcacgaattc gcgcacggac
     1021 tgcactacgc ggccgcgccg ctgacctcgt acgacgcgcc gccggtggcg gtctacgagg
     1081 gcttcgcgac gtacatcgag ctgcgcaccg ggatcaccga catccgctgg ctgcaggagc
     1141 cggacgtgaa ggatctcgtc gcgtcgcagg gtgtggcggc cctgtccgac caggcgctca
     1201 ccgagggcga ggacgcgtgg ctgtcgtaca tcgcggccgg ctcgtactac gtgttcgtcg
     1261 ccgagaacgg cggggacccg tggaagctcg cgctcgacgg agcggatgcg gtgggcaaga
     1321 acctcatcga gatcgagaac gacccgcgct tcagcgaggc gaactggaag gcgtggctga
     1381 agcgacgctg acggaccgcg gcctggttgt caatcccctt catccggatc ctcgcggatg
     1441 agagtcttgc ctctccgcaa tcgggcggcg taccgcccac cacaggaagg ggatatccca
     1501 tgggtgtcat ggacgatgcg aagcacaacg ccgagaagct ggtcggcaag gccaaggagc
     1561 acatcggcga cgcgacggac aacgaggatc tggaggccga gggcaagctc gatcaggcca
     1621 aggccagtgt caagaaggtc ggcgacgacg tcaaggacgc cttcgacaag tagtcgtcgc
     1681 tcatcgacat acgacggttc gcgaggcgga tcgtctgaga acgggcgggt gcaccgtcgg
     1741 tcccggacgg gaccgcggcg cacccgctct cagctgtgaa gggggcacgg atgccgttca
     1801 ccggtcgctt cggcatcgag gaggagttcg cactgctcga cgccttcacc ctcgtgcccg
     1861 tctccgccga gggggtccac gccgccgtgc tcggcgacgt tcccggggga ggccggctga
     1921 gcgcggagtt catgacgagc caggtggagg cggcgaccga accggtcgga tcgctcgcgc
     1981 aggcgcgcga gcagctggtg cggatgc
//
LOCUS       DATMSX010000228         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_145281,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000228 DATMSX010000000
VERSION     DATMSX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145281"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VN041_06970"
     CDS             <1..385
                     /locus_tag="VN041_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWU28819.1"
                     /translation="VADALAARVGDTTAWQEQTFIPRVAKRGAEIIEVRGSSSVASAA
                     SAAIDHVRDWVHGTDDWTSAGVVSHGEYGVPEGLICSFPVQSVEGGWRIVEGLELDAW
                     ARARVDASVAELIEERDAVRALGLL"
     gene            464..937
                     /locus_tag="VN041_06975"
     CDS             464..937
                     /locus_tag="VN041_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWU28820.1"
                     /translation="MSDVTNAKGTGSIEQGLEAALTSPLHLPHAAATCPQCLANEKHD
                     WWSARPEGTRLVGLVVARDGMPSVTQQREDLTRFGVPIEGFRHPAPEILESWGDRLAR
                     LIETLRAGDVLVVANVHALGRDRDEESRTVAELRQRGIMVKVLGHHARHLADADG"
     gene            complement(1052..1342)
                     /locus_tag="VN041_06980"
     CDS             complement(1052..1342)
                     /locus_tag="VN041_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HWU28821.1"
                     /translation="MGGNGYKMQFGAVDAAGADLVGGAGNIETKLNDMENALKPLQAD
                     WTGAASEAYVQAKAQWNSALTEMKALLHDIGRQVSQDSADGQSNENRNVNRW"
     gene            complement(1362..1673)
                     /locus_tag="VN041_06985"
     CDS             complement(1362..1673)
                     /locus_tag="VN041_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HWU28822.1"
                     /translation="MGGEVSAADGALQRGAQIVNQSKTDIVGELNSIQNKLAGIGSSW
                     TGAGAAAFQQTFQAWQDKSRRITNALDGFEQNLRDSQSAYSQTDDSSAQAHSKFQGRL
                     G"
     gene            complement(1831..>3663)
                     /gene="eccCb"
                     /locus_tag="VN041_06990"
     CDS             complement(1831..>3663)
                     /gene="eccCb"
                     /locus_tag="VN041_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCb"
                     /protein_id="HWU28823.1"
                     /translation="FTAAPVIVQETAEEPEAVPEVGGAEAPEKRVTFDIAVSLMKGRS
                     MPAHQVWLPPLDVPATFDEMLGDLVEDPQLGLISPRWRQAGPLTIPLGIVDRPLEQRR
                     DSLTMSLVGAGGHLAIVGGARSGKSTLARSVLTGIALTHTPQEVQFYVMDFGGGTFVP
                     FSKMAHVAGVAGRNQPDVLRRMFQEVMGIVNARERYFTANAIDSIETYRRLRAQGRAD
                     DGYGDVFLIIDGWPTIRAEFDEMEFEIQSLAGRALTFGVHLIVTTTRWMDFRTAIRDT
                     FGSKLELRLGDTSDSEIDRKVAANVPLGRPGRGLAPSKHHMLTALPRVDGSGDPDTLS
                     EGVEDLINRVNAAWKGPQGPKLRLLPEMLELPQLQQLAAGTPLQNQVLLGVDEAELAP
                     TAFDTRRNPHLYLFGDSQSGKSSFLRTFAHEVMRTASPKDAQFFVVDYRRALLSEIPD
                     EYLAGYFTTAQQATEELAGLTEYLRSRLPGTDVTPQQLRDRSWWSGAEVYVLVDDYDL
                     VNTQSGNPIAVLQPLLAQATDVGLHLTLVRRSGGASRATFEPVMQTLRDLASPGIMLS
                     GSPDEGPLIGNVKASPAVAGRAKVITRERGLQIMQLAYAPPSQL"
BASE COUNT          543 a         1226 c         1299 g          595 t
ORIGIN      
        1 ggtcgcggat gccctggccg cgcgcgtcgg cgacacgacg gcctggcagg agcagacgtt
       61 catcccccgc gtcgccaagc gcggtgccga gatcatcgag gtgcgcggat cgtcgtcggt
      121 ggcgtccgcg gcgagtgccg cgatcgacca cgtgcgcgac tgggtgcacg gcacggacga
      181 ctggacgagt gcgggcgtcg tctcgcacgg cgagtacggc gtgcccgagg gcctgatctg
      241 ctcgttcccc gtgcagtcgg tcgagggagg ctggcggatc gtcgagggcc tggagctgga
      301 tgcctgggcg cgcgcccgcg tggacgcttc cgtcgcggag ctgatcgagg agcgcgacgc
      361 ggtccgcgca ctgggtctgc tctgaccccg caccccttca ccgggacgca tgtcggcgga
      421 ctcgggcaga atgggagcgc agaatgggag cggaggtgcg ttcatgagtg acgtgacgaa
      481 tgcgaagggc acgggctcga tcgagcaggg cctggaagcc gctctcacga gcccgctgca
      541 cctgccgcac gccgcggcga cgtgcccgca gtgcctggcg aacgagaagc acgactggtg
      601 gtcggcacgc cccgagggca cccgcctggt cggactggtg gtcgcgcgcg acgggatgcc
      661 gtcggtgacc cagcagcgtg aggatctgac gcgcttcggc gtgccgatcg aaggcttccg
      721 gcatccggcg cccgagatcc tggagagctg gggcgaccgg ctggcccggc tgatcgagac
      781 gctgcgcgcg ggcgatgtgc ttgtcgtcgc caatgtgcac gcgctcggcc gcgaccgcga
      841 cgaggagtcg cgcacggtgg cggagctgcg tcagcgcggc atcatggtca aggtgctggg
      901 ccaccacgcc cggcatctcg ccgacgccga cggctgagct cccgcgcacg atgccgggcg
      961 cacgacgccc gccccgcacg acaagaaacc ggctgccatc cccgagggga tgacagccgg
     1021 ttcgaacgcc tgacgcgtca gtgacagaag gtcaccagcg gttgacgttg cggttctcgt
     1081 tggactggcc gtcggccgag tcctgcgaca cctgacgacc gatgtcgtgc aggagcgcct
     1141 tcatctcggt cagcgcggag ttccactgcg ccttggcctg gacgtacgcc tcggaagccg
     1201 caccggtcca gtctgcctgg agaggcttga gcgcgttctc catgtcgttg agcttggtct
     1261 cgatgttccc cgcgccgccg accaggtctg cacctgctgc gtcgacggca ccgaactgca
     1321 tcttgtagcc gttaccaccc atgttgttct tccctccgga ttcagccgag acggccctgg
     1381 aacttgctgt gggcctgcgc ggacgagtcg tcggtctgcg agtacgccga ctgggagtcg
     1441 cgcaggttct gctcgaatcc gtcgagagcg ttcgtgatac gacgcgactt gtcctgccat
     1501 gcctggaacg tctgctggaa cgccgcggcg cccgcacccg tccacgacga gccgatgccc
     1561 gcgagcttgt tctgaatcga gttcagctcc cccacgatgt cggtcttcga ctggttgacg
     1621 atctgggcgc cccgctgaag agcaccatcc gctgcagaga cttctccgcc cacggtcacc
     1681 tcctggtcct gagggctttg caagccctga ttgcttcagc cccggagggg ccgtgtcccc
     1741 gatgcccggc gactaataac accttatggt ccccgctggg ggatgcctat gggtagctct
     1801 gcccacgcag atctgcccat cggcatccgc tcacagctgg gacggcggcg cgtacgccaa
     1861 ctgcatgatc tgcagtccgc gctcacgggt gatcaccttg gcgcgacccg cgacggcggg
     1921 cgacgccttg acgtttccga tcagcggtcc ctcgtccggg cttccggaga gcatgatccc
     1981 cggggatgcc aggtcgcgca gcgtctgcat gaccggttcg aacgtggcgc gcgaggcgcc
     2041 gcccgatcgc cgcaccagcg tcagatgcag accgacgtcg gtcgcctggg cgagcagggg
     2101 ctgcagcacc gcgatcgggt tgcccgactg cgtgttgacc aggtcgtagt cgtcgacgag
     2161 cacgtacacc tctgcgccgg accaccacga ccggtcacgc agctgctgtg gcgtgacatc
     2221 ggtgccggga aggcggctgc gcaggtactc ggtgaggcct gcgagctcct ccgtggcctg
     2281 ctgcgcggtc gtgaagtacc cggcgaggta ctcatcgggg atctccgaga gcagcgcacg
     2341 gcgataatcg acgacgaaga actgggcgtc cttcgggctc gccgtgcgca tgacctcgtg
     2401 ggcgaaggtg cgcaggaagc tcgacttgcc cgactggctg tctccgaaca ggtacaggtg
     2461 cgggttgcgc cgggtgtcga acgcggtggg cgccagctcg gcctcgtcga caccgagcag
     2521 cacctggttc tgcagtggcg tgcctgccgc gagctgctgc agctgcggaa gctcgagcat
     2581 ctcgggcagc agacgcagct tggggccctg cgggcccttc caggccgcgt tcacgcggtt
     2641 gatcaggtcc tccacgccct cgctgagggt gtcggggtcg ccggagccgt ccacacgcgg
     2701 cagcgccgtg agcatgtggt gcttggacgg cgcgagaccg cgaccgggac gacccagcgg
     2761 cacgttggcg gcgaccttgc gatcgatctc ggagtcgctg gtgtcaccga gacgcagctc
     2821 gagcttggag ccgaacgtgt cccggatcgc ggtgcggaag tccatccagc gcgtggtggt
     2881 cacgatcagg tgcacgccga aggtgagcgc ccttccggcg agtgactgga tctcgaactc
     2941 catctcgtcg aactcggcgc ggatggtcgg ccagccgtcg atgatcagga acacgtcgcc
     3001 gtagccgtca tccgccctgc cctgcgcgcg caggcgccgg tacgtctcga tcgagtcgat
     3061 cgcgttggcg gtgaagtagc gctcacgggc gttgacgatg cccatgacct cctggaacat
     3121 gcgtcgcagc acgtcgggct ggttgcgacc cgcgacgccg gcgacgtgcg ccatcttcga
     3181 gaacggcacg aacgtgccgc cgccgaagtc catcacgtag aactgcacct cctgcggagt
     3241 gtgcgtcagg gcgatgccgg tcagcacgct gcgggcgagc gtgctcttgc cgctgcgcgc
     3301 accgccgacg atggcgaggt gtccgccggc gccgaccagg ctcatggtca ggctgtcacg
     3361 gcgctgctcg agcggacggt cgacgatccc gagcgggatg gtcagcgggc cggcctgacg
     3421 ccagcgtggc gagatgaggc cgagctgcgg gtcctcgacc aggtcgccga gcatctcgtc
     3481 gaaggtggcg ggcacgtcca ggggcggcag ccacacctgg tgcgcaggca tgctgcggcc
     3541 cttcatcagc gagacggcga tgtcgaaggt cacgcgcttc tcgggcgctt cggccccgcc
     3601 gacctcgggc acggcctcgg gctcctccgc cgtctcctgc acgatcacgg gcgccgcggt
     3661 gaa
//
LOCUS       DATMSX010000229         5289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_12793,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000229 DATMSX010000000
VERSION     DATMSX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12793"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="atpA"
                     /locus_tag="VN041_06995"
     CDS             <1..1171
                     /gene="atpA"
                     /locus_tag="VN041_06995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWU28824.1"
                     /translation="DSMTPIGRGQRQLIIGDRATGKTTIAIDTIINQKQNWESGDPTK
                     QVRCIYVAIGQKGSTIASVRGALEEAGALEYTTIVAAPASDPAGFKYLAPYTGSAIGQ
                     HWMYGGKHVLIIFDDLSKQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLS
                     DELGAGSMTGLPIIETKANDVSAYIPTNVISITDGQIFLQSDLFNANQRPAVDVGISV
                     SRVGGDAQVKSIKKVSGTLKLELAQYRSLEAFAMFASDLDAASRRQLSRGARLTELLK
                     QPQYSPYPVEEQVVSIWAGTNGKLDSIEVEDVLRFERELLDYLRRNTKVLDTLRETNV
                     LDDDTVAELDKQTDNFILEFQGGKGHAIGAPGHEEHAAAEAEDVNQEKIVKGRRA"
     gene            1191..2087
                     /locus_tag="VN041_07000"
     CDS             1191..2087
                     /locus_tag="VN041_07000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWU28825.1"
                     /translation="MGAQLRVYKQKISSAQTTKKITKAMELIAASRIQKAMARVKASS
                     PFARAVTRAVSAVATHSNVDHPLTREPEKISRSAVVIFSSDRGLAGAFNSQILREGLE
                     VAELLREQGKEPVFYLVGRKAVGYFQFRGIASAAEWTGDTDTPSFHTAEKISHTLLDA
                     FNRGGDAGGVDEITLVYNRFVSMMTQSPESVRLLPLEIAEADDSEAGSTVYPLYEFEP
                     DAETVLDAILPVYIQSRVFNALLQSSAAKQAATQKAMKSASDNADKLITDYTRLRNNA
                     RQAEITQQIAEIVGGADALASS"
     gene            2114..3568
                     /gene="atpD"
                     /locus_tag="VN041_07005"
     CDS             2114..3568
                     /gene="atpD"
                     /locus_tag="VN041_07005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWU28826.1"
                     /translation="MTPTATADKPATAVVGRVARVNGPVVDIEFPHDSIPDIYNALKT
                     TIVIGDESNEITLEVAQHLGDDLVRAIALKPTDGIVRGQEVRDTGEAISVPVGDVTKG
                     KVFNVIGEVLNLEPGETIEVTERWPIHRKAPNFDQLESKTTMFETGIKSIDLLTPYVL
                     GGKIGLFGGAGVGKTVLIQEMIQRVAQDHGGVSVFAGVGERTREGNDLIHEMEEAGVF
                     DKTALVFGQMDEPPGTRLRVALSALTMAEYFRDVQNQDVLLFIDNIFRFTQAGSEVST
                     LLGRMPSAVGYQPNLADEMGVLQERITSTRGHSITSLQAIYVPADDYTDPAPATTFAH
                     LDATTELSREIASKGLYPAIDPLTSTSRIMDPRYLGEDHYRVATTVKQILQKNKELQE
                     IIAILGVDELSEEDKIVVARARRIQQFLSQNTYMAKKFTGVEGSTVPLKETIESFDAI
                     CRGDFDHVAEQAFFNVGGISDVEEAWAKIQKENG"
     gene            3571..3831
                     /locus_tag="VN041_07010"
     CDS             3571..3831
                     /locus_tag="VN041_07010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWU28827.1"
                     /translation="MALHVSLVSADAEVWTGEASLVVAKTVEGEIGFMSGHEPVLAIL
                     AEGQVRITRPDGTKVLANAQDGFLSMEGDALTIVAGNAALVA"
     gene            3908..4648
                     /gene="yaaA"
                     /locus_tag="VN041_07015"
     CDS             3908..4648
                     /gene="yaaA"
                     /locus_tag="VN041_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HWU28828.1"
                     /translation="MKILLPPSETKRTGGRPRPLDVGGLALPALAPQRDAVVDALVAL
                     SADADAARRVLKLSDRQLADVDHNRMLRTAATMAAVDRYTGVLYDALDAGSLDQPARR
                     WLGRNVLIHSAPFGPIGALDPIPVYRLSAGASLPGIPPLRRHWSAATSAALAGADFVL
                     DLRSEAYVALGPIPATVPSAYVRVVTETGRALNHFNKKSKGELMRALASTRPRAKSVD
                     AVLLWAREQGILLRETADAGVLELVVAE"
     gene            4725..>5289
                     /locus_tag="VN041_07020"
     CDS             4725..>5289
                     /locus_tag="VN041_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HWU28829.1"
                     /translation="MDDYSGYGYDAGLGALIGLIWLIALLAGPIFYVITAWFMMNIFE
                     KAGVQGKWRAWVPFYNGMIFLKLGDVSPWLLFGVFVTWIPVLGWLVSIGLAVMSILAA
                     YRIGQKLQKDGAWIVLYILLAPVWMGILAFDSSRWNPNIPPAAWAGNGFLGDRTVWDG
                     IPVQPSAAAAPGFAAPGYGAPQGYQPPA"
BASE COUNT          919 a         1806 c         1725 g          839 t
ORIGIN      
        1 cgactcgatg accccgatcg gccgtggcca gcgccagctg atcatcggcg accgcgcgac
       61 cggcaagacc acgatcgcga tcgacacgat catcaaccag aagcagaact gggagtcggg
      121 cgacccgacc aagcaggtcc gctgcatcta cgtcgcgatc ggccagaagg gctcgaccat
      181 cgcctcggtg cgtggcgccc tcgaggaggc cggcgccctg gagtacacca cgatcgtggc
      241 agctcccgcg tccgaccccg ccggcttcaa gtacctggct ccgtacaccg gttcggccat
      301 cggccagcac tggatgtacg gcggcaagca cgtcctcatc atcttcgacg acctgtcgaa
      361 gcaggccgag gcctaccgcg ccgtctcgct gctgctgcgc cgcccgccgg gccgcgaggc
      421 gtacccgggt gacgtcttct acctgcactc ccgcctgctg gagcgttgcg cgaagctgtc
      481 cgacgagctc ggtgccgggt cgatgacggg tctgcccatc atcgagacca aggcgaacga
      541 cgtctcggcg tacatcccga ccaacgtgat ctcgatcacc gacggccaga tcttcctgca
      601 gtccgacctc ttcaacgcca accagcgtcc ggcggtcgac gtgggtatct cggtgtcgcg
      661 agtcggtggt gacgcacagg tcaagtccat caagaaggtc tccggaacgt tgaagctgga
      721 gttggcgcag tatcgctcgc tcgaggcgtt cgcgatgttc gcgtccgacc tcgacgcggc
      781 ctcgcgtcgt cagctctccc gtggtgcgcg tctgaccgag ctgctcaagc agccgcagta
      841 ctcgccgtac cccgtcgagg agcaggtcgt ctcgatctgg gccgggacga acggcaagct
      901 cgactcgatc gaggtcgaag acgtgctgcg gttcgagcgt gagctgctgg actacctgcg
      961 tcgcaacacc aaggtgctcg acacgctgcg tgagaccaac gtgctggatg acgacacggt
     1021 cgccgagctc gacaagcaga cggacaactt catcctggag ttccagggcg gcaagggcca
     1081 cgccatcggc gcccctggtc acgaggagca cgctgcggcc gaggccgagg acgtcaacca
     1141 ggagaagatc gtcaagggtc gtcgcgcgta atcgcgtgag gaactgacat atgggcgctc
     1201 aactcagggt ctacaagcag aagatctctt ctgctcagac gaccaagaag atcacgaagg
     1261 cgatggaact catcgcggct tcgcgcatcc agaaggcgat ggcacgcgtc aaggcgtcca
     1321 gccccttcgc gcgtgccgtg acgagggccg tgtccgccgt cgcgacgcac tcgaacgtcg
     1381 accacccgct cacccgcgag cccgagaaga tcagccgctc cgcggtcgtg atcttctcgt
     1441 cggaccgcgg tctcgccgga gccttcaact cgcagatcct ccgtgagggt ctcgaggtgg
     1501 cagagctcct gcgcgagcag ggcaaggagc cggtgttcta cctggtgggc cgcaaggccg
     1561 tcggttactt ccagttccgg ggcatcgcct cggcggcgga gtggaccggc gacacggaca
     1621 cgccgtcctt ccacaccgcg gagaagatct cgcacacgct gctggatgcc ttcaaccgcg
     1681 ggggagacgc cggcggcgtg gacgagatca ccctcgtgta caaccgcttc gtcagcatga
     1741 tgacgcagtc gccggagtcc gtgcgcctgc tgccgctgga gatcgcggag gcggacgact
     1801 cggaggcggg cagcactgtc tacccgctct acgagttcga gccggatgcc gagaccgtgc
     1861 tggacgcgat cctgccggtg tacatccaga gccgtgtgtt caacgcactg ctgcagtcgt
     1921 ccgccgcgaa gcaggcggcg acgcagaagg cgatgaagtc ggccagcgac aacgccgaca
     1981 agctgatcac cgactacacc cgcctgcgca acaacgcgcg tcaggccgag atcacccagc
     2041 agatcgccga gatcgtcggt ggcgccgacg cactggcatc cagctgacag acactcagga
     2101 aagagacgaa gaaatgaccc ccaccgctac ggctgacaag cccgcgaccg cggtcgtcgg
     2161 gcgcgtcgca cgcgtcaacg gtccggttgt cgacatcgag ttcccgcacg actcgatccc
     2221 cgacatctac aacgcgctga agacgaccat cgtcatcggc gatgagtcca acgagatcac
     2281 cctcgaggtc gcacagcacc tgggtgacga cctggtgcgc gcgatcgccc tgaagccgac
     2341 ggatggcatc gtccgcggcc aggaggtgcg cgacaccggc gaggcgatct ccgtccccgt
     2401 cggcgacgtc accaagggca aggtcttcaa cgtgatcggc gaggtcctga acctcgagcc
     2461 cggcgagacc atcgaggtca ccgagcgctg gccgatccac cgcaaggctc ccaacttcga
     2521 ccagctcgag tcgaagacca cgatgttcga gaccggcatc aagtcgatcg acctgctcac
     2581 cccgtacgtg ctgggtggaa agatcggtct gttcggtggc gccggtgtcg gcaagaccgt
     2641 cctcatccag gagatgatcc agcgcgtcgc gcaggaccac ggtggtgtgt cggtgttcgc
     2701 cggtgtcggt gagcgcaccc gtgagggcaa cgacctgatc cacgagatgg aggaggcggg
     2761 cgtcttcgac aagaccgccc tcgtcttcgg ccagatggac gagccgccgg ggacgcgcct
     2821 gcgcgtcgcc ctgtcggccc tgaccatggc ggagtacttc cgtgatgtgc agaaccagga
     2881 cgtgctgctc ttcatcgaca acatcttccg cttcacgcag gccggttccg aggtctccac
     2941 gctgctgggc cgcatgccct ccgccgtggg ttaccagccg aacctggccg acgagatggg
     3001 tgttcttcag gagcgcatca cctcgacgcg cggtcactcg atcacctcgc tgcaggcgat
     3061 ctacgtgccg gccgacgact acaccgaccc ggcgccggcg accacgttcg cgcacctcga
     3121 cgcgacgacc gagctcagcc gtgagatcgc gtcgaagggt ctgtacccgg ccatcgaccc
     3181 gctgacctcg acgtcgcgca tcatggaccc ccgctacctg ggcgaggacc actaccgcgt
     3241 ggccaccacg gtcaagcaga tcctgcagaa gaacaaggag ctccaggaga tcatcgcgat
     3301 cctcggtgtc gacgagctct cggaagagga caagatcgtc gtcgcccgcg cgcgccgcat
     3361 ccagcagttc ctctcgcaga acacctacat ggcgaagaag ttcaccggcg tcgagggttc
     3421 gaccgtgccg ctgaaggaga ccatcgagtc gttcgatgcg atctgccgcg gtgacttcga
     3481 ccacgtcgcc gagcaggcat tcttcaacgt cggtggcatc tccgacgtgg aagaggcgtg
     3541 ggcgaagatc cagaaggaga acggctgacc atggctctgc acgtcagcct cgtctccgcg
     3601 gacgcggagg tctggacggg agaagcgtcg ctcgtcgtcg ccaagaccgt cgagggcgag
     3661 atcggcttca tgtccggtca tgagccggtc ctggccatcc tcgccgaggg gcaggtccgg
     3721 atcacccggc ccgacggcac gaaggtgctc gccaacgcgc aggacggctt cctctccatg
     3781 gagggcgacg ccctgacgat cgtggccgga aacgccgcgc tggtcgcctg acaaccattc
     3841 ccgagacgtc cgcctcgacc gcgctccggc gcaggacgag gcggacgtct ccgtctgcac
     3901 ggagctcatg aagatcctcc tcccgccctc ggagaccaag cgcaccgggg gccgaccgcg
     3961 gccgctggat gtcggcggtc tcgcgctgcc cgcgctggcc ccgcagcgcg acgcggtcgt
     4021 ggacgccctc gtggcgctgt cagcggatgc cgacgccgcg cggcgcgtgc tcaagctcag
     4081 cgaccgtcag ctggcggatg tggaccacaa ccggatgctg cgcacggccg ccacgatggc
     4141 ggccgtcgac cggtacaccg gcgtgctcta cgacgcactg gacgccggat cgctcgacca
     4201 gcccgcccga cggtggctgg gacggaacgt gctgatccat tccgcgccct tcggcccgat
     4261 cggcgcgctg gacccgattc cggtgtaccg gctgtcggcc ggggcctcgc tcccgggcat
     4321 cccgccgctg cgacggcact ggagcgcagc gaccagcgcc gcgctggccg gcgcggactt
     4381 cgttctcgac ctgcgcagcg aggcgtacgt cgcgctcggg ccgatacccg cgacggttcc
     4441 ctccgcgtac gtgcgcgtgg tcaccgagac gggccgggcg ctcaaccact tcaacaagaa
     4501 atccaagggc gagctgatgc gggccctcgc ctcgacacgc ccgagggcga agtcggtcga
     4561 tgccgtcctc ttgtgggccc gcgagcaggg catcctgctc agggagacag cggatgccgg
     4621 agtgctggag ctcgtcgtcg cagagtgacg attgcccgcg gtaccgggcg ttggctagtg
     4681 tggtcacggc gtccgcgtgg acgcgaatgc ctacggaggg aaacatggac gactacagcg
     4741 ggtatggcta tgacgccggg ttgggcgccc tgatcggtct gatctggctg atcgcgctgc
     4801 ttgccggccc catcttctac gtcatcacgg cctggttcat gatgaacatc ttcgagaagg
     4861 caggcgtgca gggcaagtgg cgtgcctggg ttccgttcta caacgggatg atcttcctga
     4921 agctcggcga tgtcagcccc tggctgctct tcggcgtgtt cgtcacctgg atccccgtcc
     4981 tcggctggct ggtgagcatc ggcctcgctg tgatgagcat cctcgcggcc taccgcatcg
     5041 gtcagaagct acagaaggac ggcgcgtgga tcgtgctgta catcctgctc gctccggtct
     5101 ggatgggaat tctcgccttc gactcgtcgc gctggaaccc gaacatcccg ccggcggcct
     5161 gggcgggcaa cggcttcctc ggtgaccgca ccgtctggga cggcatcccc gtgcagccgt
     5221 cggctgccgc tgctcccggc ttcgccgccc cgggctacgg cgccccgcag ggttaccagc
     5281 cgccggcac
//
LOCUS       DATMSX010000230         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_50791,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000230 DATMSX010000000
VERSION     DATMSX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50791"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VN041_07025"
     CDS             complement(<1..825)
                     /locus_tag="VN041_07025"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWU28830.1"
                     /translation="MIHVVRHRGWPADASPSALPEPAEAALGITVTDRAVLRDGVPFV
                     PVSGEIHFSRLPRERWGERLRQMRANGVTLASAYVLWLHHSPRRGIAHFDGALDVAAF
                     IDECAAVGLGVVLRIGPWAHGEARNGGFPDWVQDAEVEHRSDDPRYLELVAEWFAQLA
                     AHLDGRARPGGPVLAIQLENELYDRPGHLVTLKRLAREAGMSAPLWTATAWGGADLPA
                     GEVMPLFGGYGDGFWADPQDDWDPSFRAHYFPSHVWDDPGVGADVRAAQGFDAAASG"
     gene            complement(822..1790)
                     /locus_tag="VN041_07030"
     CDS             complement(822..1790)
                     /locus_tag="VN041_07030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWU28831.1"
                     /translation="MSSYSAAFDWARRHVNAERLPTAVLGVATTDGIVALDGFGAPTD
                     AVYPLFSITKVLTGITAARAVERGLLTLETPLASALPDFGATRDDVVRLRHLVSHTSG
                     ITEPALDTDVPLRIELLTRGRDFAAGTASRYSTLAFEGIAALTEQATGTTWDDDTRRW
                     AATVGADGLTLDHEGSMGIPDASAGGLDLDRFRATRAPGAGLLGRAEDLLRIGSELLR
                     IHAGRTDGILAPATLDMMLRPLTGDIPRLEPYPVERGQDWGFTFNLRSRAPGLIDRDA
                     YGHGGWAGTEFWVHPGAGLCWVLLTNRAVREGVDADQLDNAVVSGR"
     gene            complement(1790..3541)
                     /locus_tag="VN041_07035"
     CDS             complement(1790..3541)
                     /locus_tag="VN041_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HWU28832.1"
                     /translation="MTSRFTIGETDFLLDGAPHRIISGAMHYPRVHPDLWRDRIRKAR
                     LMGLNAIETYVAWNAHEPVKGQWREDGDVDLGRFLDIIAEEGMHAIVRPGPYVCAEWH
                     NGGLPTWLTADKSMRLRSSDPAYLAEVTAYVEKVNAIVAPRQIDRGGSVILVQIENEY
                     GAYGADKAYLEALIRTTRDNGITVPLTQVDQPIAHMLQGGAHPELHMTGSFGSRIDER
                     LATLREFQPAGPLMCMEFWCGWFDHWGEKHHTTAFADSAADLDRLLAAGASVNIYMVH
                     GGTNFALTNGANDSGRYLPMVTSYDYDSPISESGDLTEKFHAFREVIARHAPVPDLPA
                     VEPVEAPTPSAAFESAGDPLELVAVDRGGFPTPPTMDELGSDVVLVEYTTDAAGSGVL
                     EAEVRDYAWVERDGAPVGRLQRGIGDLSLAVPGGRTLRMLVEETGRVNYDQKIGEAKG
                     LIGSPLIDGTPLDGPWRVRTFDVPALGAAVSEAADATPVASGRVAGPVGLRAGFDLDE
                     PADLFLDTAGWGKGYAWINGFFLGRYWRRGPQRTLYVPGPVTRAGRNEIVVVELESMT
                     DATARFLARPDLGHEVE"
     gene            complement(3734..>4521)
                     /locus_tag="VN041_07040"
     CDS             complement(3734..>4521)
                     /locus_tag="VN041_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HWU28833.1"
                     /translation="LWYAEARAASLPVSGVLGRRRDSVRAYGSGVNLHYSLEELVAQV
                     RRWTDAGFGAVKIKVGKPTLDEDIERVAAVREALGPDRELMIDANQRWDLDRATRSIE
                     ALSAFDLTWIEEPLRADDLAGHTELGRRLAASSRVPIALGENLHNAHRFGDFLRAGAA
                     QIVQPNIVRVGGITPFLRIAELAGEHGAAAHPHLLPELSGQLALTLPGTVAEPWAEDV
                     EDAGFGDLGALIDPSPVATAAGRLSEVPHLGLGIRFRDSRPDR"
BASE COUNT          652 a         1578 c         1649 g          642 t
ORIGIN      
        1 cccggatgcc gccgcgtcga agccctgcgc ggcccgcacg tcggcgccga cgccgggatc
       61 gtcccagacg tgcgagggga agtagtgcgc acggaacgac ggatcccagt catcctgcgg
      121 gtcggcccag aagccgtcgc cgtagccgcc gaacagcggc atgacctcgc cggcgggcag
      181 gtcggccccg ccccaggccg tcgcggtcca cagcggtgcc gacattccgg cctcgcgtgc
      241 caggcgcttg agcgtgacca gatggcccgg gcggtcgtag agctcgttct cgagctggat
      301 cgccagcacg ggcccgccgg gtcgggcacg cccatccaga tgtgcggcga gctgcgcgaa
      361 ccactccgcc acgagttcca ggtagcgcgg gtcgtcgctg cggtgctcga cctcggcatc
      421 ctgcacccag tcggggaagc cgccgttgcg cgcctccccg tgcgcccagg gaccgatccg
      481 cagcaccacg ccgagtccga ccgcggcgca ctcgtcgatg aatgcggcga cgtcgagagc
      541 gccgtcgaag tgcgcgattc ctcggcgagg ggagtggtgc agccagagca cgtaggcgga
      601 tgccagcgtc accccgttcg cacgcatctg ccgcagccgc tcaccccacc gttcgcgggg
      661 gaggcggctg aagtggatct ctcccgagac cggaacgaag ggcacgccgt cgcgcagcac
      721 ggcgcggtcg gtcacggtga tcccgagtgc tgcctccgcc ggttcgggca gggcgctggg
      781 ggaggcgtcg gcgggccagc cgcgatgtcg cacgacgtgg atcatcgacc cgagaccacg
      841 gcgttgtcga gctggtcggc gtcgacgccc tcgcgcaccg cgcggttcgt cagcagcacc
      901 cagcacaggc cggcgcccgg gtgcacccag aactccgtgc ccgcccagcc gccgtgcccg
      961 tacgcgtcgc gatcgatgag accgggcgcg cgggagcgca ggttgaacgt gaagccccag
     1021 tcctgccccc gctccacggg gtacggctcg agtcgcggga tgtcgccggt gagcgggcgc
     1081 agcatcatgt ccagcgtcgc gggagcgagg atgccgtcgg tcctgccggc gtggatgcgc
     1141 agcagctcgg agccgatgcg cagcaggtcc tcagcgcgcc cgagcaggcc cgctccggga
     1201 gcgcgggtgg cgcggaagcg atccagatcg agtccgccgg cggaggcatc cgggatgccc
     1261 atcgagccct cgtggtcgag cgtgaggccg tcggcgccga cggtcgccgc ccaccgccgg
     1321 gtgtcgtcat cccaggtcgt gccggtggcc tgctcggtga gtgcggcgat gccctcgaag
     1381 gcgagcgtcg aataccggga tgccgtgccg gcggcgaagt cgcggccccg ggtgagcagc
     1441 tcgatgcgca gcggaacgtc ggtgtcgagc gccggctcgg tgatgccgga ggtgtggctg
     1501 acgagatggc gcaggcgcac cacgtcgtcg cgggtcgccc cgaagtccgg cagggcggat
     1561 gccagcggag tctcgagcgt gagcagcccg cgctcgaccg cccgtgcggc ggtgatgccg
     1621 gtgagcacct tggtgatcga gaacagcggg tagacggcat ccgtcggggc cccgaacccg
     1681 tcgagcgcga cgattccgtc agtggtcgcg acaccgagca cggccgtggg cagcctctcg
     1741 gcgttgacgt gccggcgcgc ccagtcgaag gctgcggagt acgacgacat cactccacct
     1801 cgtggccgag gtcggggcgg gcgaggaagc gcgcggtggc atcggtcatc gattccaact
     1861 ccacgacgac gatctcgttc cggcccgcgc gcgtgaccgg acccggcacg tagagcgtgc
     1921 gctgcggccc acggcgccag tacctgccga ggaagaagcc gttgatccag gcgtagccct
     1981 tgccccagcc ggcggtgtcg aggaagagat ccgcgggctc gtcgagatcg aacccggcgc
     2041 gcaggcccac ggggcccgcg acgcgcccgc ttgcgacggg tgtggcgtca gcggcctccg
     2101 acacggccgc gcccagcgct ggaacgtcga aggtgcgcac gcgccagggt ccgtcgagcg
     2161 gcgtcccgtc gatcagtggc gagccgatca ggcccttcgc ctcgccgatc ttctggtcgt
     2221 agttgacccg gccggtctcc tccaccagca tccgcagggt ccgtcccccg ggcaccgcca
     2281 gcgacaggtc gccgatgccg cgctgcaggc ggccgaccgg tgcgccgtcg cgctcgaccc
     2341 aggcgtagtc ccgcacctcg gcctcgagca cgccggagcc tgcggcatcc gtcgtgtact
     2401 cgaccagcac gacgtcggag ccgagctcat ccatggtcgg cggcgtcggg aatccgccgc
     2461 gatcgacggc caccagctcg aggggatcgc cggccgactc gaacgcggcg gacggcgtgg
     2521 gagcctcgac cggttcgacg gcggggagat ccggcaccgg ggcgtgcctc gcgatcacct
     2581 cgcggaaggc gtggaacttc tcggtcagat cgccggactc gctgatcggg gagtcgtagt
     2641 cgtagctcgt caccatcggc aggtagcggc cggagtcgtt cgcgccgttc gtcagggcga
     2701 agttcgtacc gccgtgcacc atgtagatgt tgacggatgc ccctgccgcc agcagccggt
     2761 ccagatcggc ggcgctgtcg gcgaaggcgg tggtgtggtg cttctcgccc cagtggtcga
     2821 accagccgca ccagaactcc atgcacatga gcggacctgc gggctggaac tcgcgcaggg
     2881 tggcgaggcg ctcgtcgatc cggctgccga aggaaccggt catgtgcagc tcggggtgcg
     2941 cgccgccctg gagcatgtgc gcgatgggct ggtccacctg ggtgaggggc accgtgatgc
     3001 cgttgtcgcg ggtcgtgcgg atcagcgcct cgagataggc cttgtcggcg ccgtaggccc
     3061 cgtactcgtt ctcgatctgc acgaggatca cgctgccgcc gcggtcgatc tgacgcggtg
     3121 cgacgatcgc gttgaccttc tcgacgtagg cggtcacctc ggcgaggtac gccgggtcgc
     3181 tgctgcgcag tcgcatgctc ttgtcggcgg tcagccacgt cggcaggccg ccgttgtgcc
     3241 actcggcgca cacatacgga ccgggccgca cgatcgcgtg catcccctcc tcggcgatga
     3301 tgtcgaggaa gcgcccgagg tcgacgtcgc cgtcctcccg ccactggccc ttgaccggct
     3361 cgtgcgcgtt ccacgcgacg taggtctcga tcgcgttcag ccccatcagg cgcgccttgc
     3421 ggatgcggtc gcgccacagg tcggggtgaa cacgcgggta gtgcatggcg ccggagatga
     3481 tccggtgcgg ggcgccgtcg agcaggaagt cggtctcgcc gatggtgaag cgggaggtca
     3541 tgcgctcagc gtatcggaaa gcgctttctg aacgacagtc ctgggcggga gtcagccctt
     3601 cggtttcgga tgcgtgcctc agaacaggag gatcgctcag agcaggacga tccggcgctg
     3661 gatcgtcctg tctccggcgt tcgtcctgtg ctcgatgacg tcggcctcgg acgagccggc
     3721 gggagcgcga tgctcagcga tccggccgtg aatcccggaa ccggatgccg agaccgagat
     3781 gcggtacctc gctcaaccgc cccgcggcgg tcgccaccgg tgaggggtcg atgagggcgc
     3841 cgaggtctcc gaacccggca tcctccacgt cctcggccca gggctccgcg acggtgccgg
     3901 gaagagtcag cgcgagctga ccggacagct ctggcagcag gtgcggatgc gcggcggcac
     3961 cgtgctcgcc ggccagctcg gcgatgcgca ggaacggcgt gatgccgccg acgcgcacga
     4021 tgttcggctg cacgatctgc gcggcaccgg cgcgcaggaa gtcgccgaac cgatgcgcgt
     4081 tgtgcaggtt ctcgcccagc gcgatcggca cgcgactgga tgccgccagc cggcggccga
     4141 gctcggtgtg gccggcgagg tcgtcggcgc gcaggggctc ctcgatccac gtgaggtcga
     4201 acgcagacag cgcctcgatc gaccgggtcg cgcggtccag gtcccagcgc tgattcgcgt
     4261 cgatcatcag ctcgcgatcc gggcccagcg cctcgcgcac ggccgccacg cgctcgatgt
     4321 cctcgtccag tgtcggcttg ccgaccttga tcttgacggc gccgaacccg gcatccgtcc
     4381 accgccgcac ctgcgcgacc agctcctcca gcgagtagtg caggttcacg cccgaaccgt
     4441 aggcgcgcac ggagtcgcgc cgccggccca gcacgccgga cacgggcaag gacgccgcgc
     4501 gcgcctcggc ataccacagt g
//
LOCUS       DATMSX010000231         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_22020,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000231 DATMSX010000000
VERSION     DATMSX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22020"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VN041_07045"
     CDS             <1..1017
                     /locus_tag="VN041_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28834.1"
                     /translation="ADAAGPASARRPRAAILLLIVAMFGLTLTLFAPMSVRMLSGRPD
                     AADIAFAIIGVLIPLGMLAVPVWIAARWLAEDRRRVRLRGFAAANGWTYRFWARSGGA
                     VGPAFEAGGDENLFDVLRTADRGVEFGRYKSVTGTGRSRTVRTTEYVTFSVAAELPHI
                     VLDSRANDGPFGRSNLVLDPVRDQHLKLEGDFDRSFRMYCPTGYERDALYLLPPDIMQ
                     SLLTHARALDVELRGSTVRLYAPQGIITTDGDSWRRLLDTVESIRDMARQWAQWRDDR
                     LMPEDAVGLSPGARPLGVARQGRRLRIRFPWFAFVFGLVGLIVWLISVISEAAEGIAG
                     WLGG"
     gene            1053..1790
                     /locus_tag="VN041_07050"
     CDS             1053..1790
                     /locus_tag="VN041_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HWU28835.1"
                     /translation="MSIPAAPVDVPDRVRDLAGDARLEPVWANDIGGLTFRASPAAGH
                     PFYIKWGPRNDECNVRDEAERMRWAGRWIRVPEVIDQGQDASHEWLVTRAIDGDSAVH
                     PRWVARPEVAVRAVGEGLRALHDALPVDDCPWDWAVESRIANAERRGIHVPERFHDAP
                     PIDRLVVCHADACCPNTLIGDDGRWLAHVDLGLLGVGDRWGDIAVASMSTEWNYGPGW
                     EDALIEAYGLEPDRERLAYYRELWNAT"
     gene            complement(1813..2577)
                     /locus_tag="VN041_07055"
     CDS             complement(1813..2577)
                     /locus_tag="VN041_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWU28836.1"
                     /translation="MTRSAWLDGVNPVTKLVLALLLSVPLFASIDPVSALVAIALQLL
                     CLPLTGHAVRTVLRRLLPIALFSPIAGLSMLLYAEPEGHVFWRFGVAAISEGSIELAI
                     AVTLRVIALGLPTILLFGRTDPTELGDALAQVAHLPSRFVLGVLAGTRMLGLFLDDWR
                     TMALARRARGVGDHGALRRFFSMAFVLLVFAVRRGTKLAMAMEARGFGSGLPRTWSRP
                     SRLHPRDAVAVLGGVAIMVIAIAAAVASGVFRFVWS"
     gene            complement(2619..>3199)
                     /locus_tag="VN041_07060"
     CDS             complement(2619..>3199)
                     /locus_tag="VN041_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28837.1"
                     /translation="GPNGAGKSTLALTLAGLLPELEGAVTAASDLAGRSGRRPSRWTS
                     RELLTRIGTVFQEPEHQFLARTLRDELAVGPRALKKPDAEATAIADDLLERLQLAPLA
                     LANPFTLSGGQKRRLSVATVLASAPQVIVLDEPTFGQDRRGWIGIVELLQEEIAAGRT
                     VVAVSHDASVVRHLGGHRVELAVAASASGGVR"
BASE COUNT          473 a         1130 c         1165 g          431 t
ORIGIN      
        1 gccgacgccg ccggtccggc gagcgcacgc cggccccgcg cggccatcct gctgctcatc
       61 gtggcgatgt tcgggctgac gctgacgctc ttcgccccga tgtcggtgcg gatgctgtcc
      121 ggccgaccgg atgccgccga catcgcgttc gcgatcatcg gagtgctcat cccgctcggc
      181 atgctcgccg tgccggtgtg gatcgccgca cgctggctgg ccgaggaccg gcggcgggtg
      241 cgcctgcgtg gcttcgcggc ggcgaacggc tggacctatc gcttctgggc gcggtccggc
      301 ggcgccgtcg gcccggcgtt cgaggccggc ggtgacgaga acctgttcga cgtgctgcgc
      361 accgccgatc ggggcgtcga gttcggccgc tacaagtcgg tgacgggcac cgggcgcagc
      421 cgaacagtca ggaccaccga gtacgtgacc ttctcggtcg cggccgagct gccgcacatc
      481 gtgctcgaca gccgcgcgaa cgacggcccg ttcgggcgca gcaacctggt gctcgatccg
      541 gtgcgcgatc agcacctcaa gctcgagggc gacttcgacc gctcgttccg gatgtactgt
      601 ccgaccggct acgagagaga tgccctgtac ctgctgcctc ccgacatcat gcagtcgctg
      661 ctgacgcatg cccgcgcgct ggacgtggag ctgaggggtt cgaccgttcg cctctacgcc
      721 ccgcagggca tcatcacgac cgatggcgac agctggcggc gactgctcga cacagtcgag
      781 tcgatccgcg acatggcgcg ccagtgggcg cagtggcgcg atgacaggct catgccggaa
      841 gacgcggtcg ggctgtcccc cggcgcacgc ccgctcggcg tcgcccggca gggtcgacgg
      901 ctgcgcatcc gcttcccctg gttcgcgttc gtcttcgggc tcgtcggact catcgtgtgg
      961 ctgatctcgg tcatcagcga ggccgccgag ggcatcgccg gctggctggg cggctgagcg
     1021 cgcccgcacc ggtgacgggt caggatggat gcatgtccat tcctgcagca cccgtcgacg
     1081 tccctgaccg tgtgcgcgac ctggccggtg acgcccggct cgaacccgtg tgggcgaacg
     1141 acatcggcgg gctgaccttc cgcgcgagcc ccgccgccgg gcatccgttc tacatcaagt
     1201 ggggcccgcg caacgacgag tgcaacgtgc gcgacgaggc cgagaggatg cgctgggcgg
     1261 ggcgctggat ccgggtgccg gaggtcatcg accagggcca ggatgccagt cacgagtggc
     1321 tggtcacgag ggcgatcgat ggcgacagcg cggtgcaccc gcgctgggtg gcgcgccccg
     1381 aggtcgcggt gcgcgcggtc ggcgagggcc tgcgggcact gcacgatgcg ctgcccgtcg
     1441 acgactgccc gtgggactgg gcggtcgaga gccgcatcgc gaacgccgag cgccgaggca
     1501 tccacgtccc cgagcgcttc catgacgccc cgccgatcga ccgtcttgtc gtctgccacg
     1561 cggatgcctg ctgcccgaac accctgatcg gcgacgacgg ccgctggctg gcgcacgtcg
     1621 acctcggcct gctcggcgtg ggcgatcgct ggggcgacat cgcggtcgcc tcgatgagca
     1681 ccgagtggaa ctacgggccc ggctgggagg acgcgctcat cgaggcgtac ggcctcgaac
     1741 ccgaccgcga gaggctggcg tactaccggg agctgtggaa cgcgacctga gtcgaccgcc
     1801 gacggagccc tgtcagctcc acacgaagcg gaagacgccg gatgccacgg ctgcggcgat
     1861 cgcgatcacc atgatggcca ccccgcccag caccgcgacg gcgtcgcgcg gatgcagccg
     1921 cgagggccgc gaccacgtgc gcgggagacc ggagccgaac ccgcgcgcct ccatggccat
     1981 cgccagcttg gtgccacggc gcacggcgaa gacgagcagc acgaacgcca tcgagaagaa
     2041 gcggcgcagc gcaccgtgat cgccgacacc gcgcgcccgg cgtgccagcg ccatcgttcg
     2101 ccagtcgtcg aggaacagcc ccagcatccg cgtgccggcg agcacgccca gcacgaagcg
     2161 gctcggcagg tgagcgactt gggcgagcgc atcgccgagc tcggtggggt cggtgcgtcc
     2221 gaagagaagg atggtcggca gacccagcgc gatcacgcgc agtgtgacgg cgatggccag
     2281 ttcgatcgag ccctcgctga tggcggcgac gccgaatcgc cagaacacgt gcccctcggg
     2341 ctcggcgtac agcagcatgc tcagccccgc gatcggcgag aacagcgcga tgggcagcag
     2401 ccggcgcagc accgtgcgca ctgcatggcc ggtgagggga agacacagca gctgcagcgc
     2461 gatcgcgacc agcgcgctga ccgggtcgat cgatgcgaac agcgggaccg agagcagcag
     2521 cgccagtacg agcttggtca ccgggttgac gccgtcgagc caggcggagc gcgtcatgcc
     2581 ggtgagagcc gatgccgggg gctgcgccct cacgacggtc acctcacccc tccggatgcc
     2641 gaggcggcga cggccagttc cacccgatgc ccgccgagat gccgcacgac cgacgcatcg
     2701 tggctcaccg cgaccaccgt gcggcccgcg gcgatctcct cctgcagcag ctcgacgatg
     2761 ccgatccacc cgcgccggtc ctgtccgaag gtcggctcgt cgagcacgat cacctgcggc
     2821 gcggaggcga gcacggtggc caccgacagc cgccgcttct gcccgccgct gagcgtgaac
     2881 gggttggcga gcgccagcgg ggccagctgg agccgctcca gcaggtcgtc ggcgatggcg
     2941 gtggcctcgg catccggctt cttcagtgct cgcggaccga ccgcgagctc gtcgcgcagc
     3001 gtgcgggcga ggaactggtg ctcgggttcc tggaacaccg tgccgatgcg ggtgagcagc
     3061 tcgcgcgagg tccagcgcga cgggcgcctg ccggagcgtc cggccagatc ggatgccgcg
     3121 gtcaccgcgc cctcgagctc gggcagcagg cccgcgagcg tgagcgccag ggtggacttg
     3181 ccggccccgt tcggcccgg
//
LOCUS       DATMSX010000232         5517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_117226,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000232 DATMSX010000000
VERSION     DATMSX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117226"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VN041_07065"
     CDS             complement(<1..935)
                     /locus_tag="VN041_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU28838.1"
                     /translation="MTDPAVTGAERLLSRLRRLTEIESPSRDVGGAREMAALLAQWWT
                     DAGARVRLVESDAGPHLVAEVDGAGDPLLLVGHTDTVWPQGTLEGEVPWGVDGDVVRG
                     PGVYDMKSGLVAMIAAVERLRGRPRRAVRAVLVCDEEIGSPLSQDLMRECAAGVAGAI
                     GFESPHPDGAMKVGRRGSTRLRLQVRGRAAHAALDPDAGISAIDELVDQLMVIRSIIA
                     DPALDSEVLCNVGTIDGGGRANVVPAEAAAEIGLRFLDGATEERVLGALRALRPVRAG
                     AEVETTVLSHRPAWSPSAADQRLLARVADAGARIG"
     gene            1047..1811
                     /locus_tag="VN041_07070"
     CDS             1047..1811
                     /locus_tag="VN041_07070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28839.1"
                     /translation="MHHASERRYQCATLRNHAELHAAADLYARVFDYETPDLHLNTNL
                     LSALVRNGGSAVGARAEDGELVGFAYGFAGRDRAGHEFHYSQAAVVDPAHQGSGVGRL
                     LKAAQRDVALGWGQRRMRWTFDPSLARNAHFNFSALGAEGVDYADDYYGRPGTDRIVV
                     EWALDRRDDPYAAARSLRPPEFRPEDWGRPVPAEDDAIWLPVPAQPGDDPVVCRAVRS
                     GLRGLVAEGRVLVACTRVDDRTAAHLAVRRLDEEEE"
     gene            1808..2518
                     /locus_tag="VN041_07075"
     CDS             1808..2518
                     /locus_tag="VN041_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWU28840.1"
                     /translation="MKANKSLSAATNEEDAHDRTLVSPGERYGERIRTNVSAEALQAR
                     VIEAETRSLAQTFDELSRTGDVPRAAALILGARRRYIGGQGKAAAYAELLGADLSATL
                     SNVFLVDGRALTPLTVLTDVRASDVLVVFSMRRYREDTVRFGELFRESGGQLVVITDS
                     ADAPLAAVASALIRVHTGSASYADSPTSVAAVCHLLSTLTTASAKGARRRLTVRDRFG
                     RDLGLYHAGPAAGAEPRG"
     gene            2515..3639
                     /gene="menC"
                     /locus_tag="VN041_07080"
     CDS             2515..3639
                     /gene="menC"
                     /locus_tag="VN041_07080"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HWU28841.1"
                     /translation="MRIDRLRLFEVALPLVHGFQTSSHRKTALDHILVEATDAEGRTG
                     WGEIASPADPFYGSETTETAWTIALRHLVPAVLGVEWDTPEQLEASWRRVRGHEFAKA
                     GFSSAGWDLHARAQGLSLATALGGSRTEVLAGVSLGIEPTIDDLLAQVERQRDAGYGR
                     VKLKIAPGWDVEPVRAVRAAHPQLDLHVDANGAYPDDDASAEVFRVLDEAELTMIEQP
                     FAPRDFVAHARLQQSIETPICLDESVVDLGDLRTMLALDAGRILNIKVSRMGGLSVAR
                     AAHDLAAEAGVPVWCGGMHEYGVGRAANVAISSLPNFTLPSDVSGSDKYFTRDVVTPP
                     VVAHEGRVTVPTSPGIGHDIDLEWVRQNTSRSFDTDRSDG"
     gene            3636..5441
                     /locus_tag="VN041_07085"
     CDS             3636..5441
                     /locus_tag="VN041_07085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWU28842.1"
                     /translation="MIPVVDGHNDLAWERRERFGSTTTGLDASVPTLHTDLPRLVAGG
                     VGGQFWSVWVDPELVGAEQVTATLEQIDFVQRLIAAYPDRLVAARTAADVRTAMAEGR
                     IASLIGVEGGAQIDGSLAVLRQYARLGARYMTLTWSRTLAWADSATDEPQHGGLTDFG
                     RDVVREMNRIGMLVDLAHVAPTTMRDALAVSTRPVVVTHSCALALCDHPRNVPDDVLA
                     MIGAQGGVVMVAFVPSFVSQARRDWVLAGEHGEPPVVGIADVADHVEHVRDVAGVHAV
                     GLGADYDGTGSMPSGLEDVSRYQSLLEELRTRGWSQQDLEAVAHGNVLRVLEASDTDH
                     AAFLAGTAGEPVASTPSVDIAQRTAARAPRVLVVVNAESSGPRRLGRWLEEDGIVVDA
                     ALGSDGLPDDLSGYDGLVMLGGGLMPDDDDRAPWLAQERVLAREAIDADLPTLGICLG
                     GQLLAHVAGGEVRASFGPKERGATLITPSSQGSSDALLSALGDAVHMIENHQDMITAL
                     PPDAVLLASSEAVENQAFRLGAHVRGLQFHPEVGAEDLTRWEEPTTRAEGDRPLAQLL
                     AEARDVDQLNTRASRAMATAFAAEVRAAAATRIAG"
BASE COUNT          799 a         1996 c         1900 g          822 t
ORIGIN      
        1 tgaccgattc gcgcgccggc atccgccacc ctcgcgagca gccgctggtc ggcggcagag
       61 ggactccagg cgggtcgatg actgagcacg gtcgtctcga cctccgctcc ggcgcgcact
      121 ggccgaaggg cgcgcagcgc accgagcact cgctcctcgg tcgcgccgtc caagaagcgc
      181 agcccgatct cggctgcggc ctcggcgggc accacgttcg cgcgtccgcc gccgtcgatc
      241 gttccgacgt tgcagagcac ctccgagtcc agggcgggat ccgcgatgat gctccggatc
      301 accatgagct ggtcgaccag ttcatcgatc gccgagatgc ctgcgtccgg gtcgagagca
      361 gcgtgggccg cccgtccgcg cacctgcaga cgcagccggg tgctgccacg tcttcccacc
      421 ttcatcgcgc cgtccggatg cggcgattcg aatccgatcg cgccggcgac gccggccgcg
      481 cactcccgca tcaggtcctg cgacaggggc gagccgatct cctcgtcgca caccagcacg
      541 gcacgcactg cgcgccgggg ccggccgcgc agccgctcga cagccgcgat catcgcgacc
      601 aggccgctct tcatgtcgta gacgccgggg ccgcgcacga catcgccgtc cactccccac
      661 gggacctctc cctcgagggt gccctggggc cagaccgtgt cggtgtgacc gaccagcagc
      721 agcggatcgc ccgcgccgtc gacttcggcg acgaggtgcg ggcccgcgtc gctctccacc
      781 aggcgcaccc gcgcgccggc atccgtccac cactgcgcga gcagggcagc catctccctg
      841 gcgccgccga catcccgcga gggggactcg atctccgtca atcgacgcag cctgctcagc
      901 aggcgctccg cgcccgtcac cgcgggatcc gtcatgctcg cccccttcgt gcgatgttat
      961 gaaccatatc tgatcattgc ttcgcccctc atcctccgat atcttcaacc ttcggatgga
     1021 aatcagcgca atataggata cgtatcatgc accacgcatc ggagcgaagg taccagtgcg
     1081 cgacactgcg caaccatgcg gagctgcacg cggccgccga tctgtacgcg cgcgtgttcg
     1141 actatgagac ccctgatctg catctgaaca ccaacctgct cagcgccctc gtgcgcaacg
     1201 gcggatcagc cgtgggcgct cgcgccgagg acggcgagtt ggtcggcttc gcgtacgggt
     1261 tcgccggccg cgaccgcgca ggtcacgagt tccactactc gcaggccgcg gtggtcgacc
     1321 ccgcccacca ggggtccggg gtcgggcggc tgctcaaggc cgcccagcgc gacgtcgccc
     1381 tgggctgggg acagcgccgc atgcgctgga ccttcgaccc ctcgctggcg cgcaacgcgc
     1441 atttcaattt cagcgcgctc ggcgccgagg gcgtcgacta cgcggacgac tactacggcc
     1501 gcccaggcac cgaccgcatc gtggtggagt gggcgctgga ccgccgagac gacccctacg
     1561 ccgccgcacg gtcgttgcgg cccccggagt tccgtcccga ggactggggc cggccggttc
     1621 cagccgaaga cgacgcgatc tggctgccgg tccccgcgca gcccggcgac gatccggtcg
     1681 tctgccgtgc ggtgcgctcg gggctccgcg gactcgtcgc cgagggccgc gtactcgtcg
     1741 cgtgcacgag agtcgacgac agaactgccg cgcacctcgc ggtgcgccgc ctcgatgagg
     1801 aggaagagtg aaagcgaaca agtccctttc ggcggccacg aacgaggagg acgcccacga
     1861 tcggacgctg gtctcacccg gcgagcgcta cggcgagcgc atccgcacga atgtgtcggc
     1921 cgaagcgctg caggcgcgcg tcatcgaggc ggagacccgg tccctggcgc agaccttcga
     1981 cgagctctcc cgcacgggcg acgtgcctcg cgccgccgcc ctgatcctcg gcgcccgtcg
     2041 ccgctacatc ggcggacagg gcaaggccgc ggcgtacgcg gaactgctcg gcgccgacct
     2101 ctcggcgacc ctctcgaacg tgttcctggt cgacggccgc gcactgaccc cgctcaccgt
     2161 gctgaccgac gtgcgcgcca gcgatgttct cgtggtgttc tcgatgcgtc gctaccggga
     2221 ggacacggtg cgcttcggcg agctgttccg cgaatcgggc ggccaactgg tggtgatcac
     2281 cgacagcgcg gatgcgccgc tggcggccgt cgcctcggct ctcatccgcg tgcataccgg
     2341 ttcggcctcg tacgccgact cccccacctc ggtcgccgca gtatgtcacc tgctctcgac
     2401 tctcaccact gccagtgcca agggggcacg ccgccggctg accgtgcggg accggttcgg
     2461 cagagatctg ggcctgtacc acgccggccc ggccgcggga gcggagccac ggggatgagg
     2521 atcgatcgac tgcgtctctt cgaggtcgcg ctcccgctcg tgcatggatt ccagaccagt
     2581 tcacatcgca agaccgccct cgaccacatc ctggtcgagg cgacggacgc cgagggccgg
     2641 acgggctggg gcgagatcgc ctcccccgcc gacccgttct acggctcgga gaccacggag
     2701 acggcgtgga cgatcgcgtt gcggcatctg gtaccggccg ttctcggcgt cgagtgggac
     2761 actcctgagc agctcgaggc gagctggcgc cgcgtccgcg gccacgagtt cgcgaaggcc
     2821 gggttctcgt ccgccggctg ggatctgcac gcccgcgccc agggcctctc gctcgcgacc
     2881 gcgctcggcg gcagccgcac cgaggtcctc gccggggtgt cactcggcat cgagccgacc
     2941 atcgacgacc tcctcgcaca ggtggagcgt cagcgggatg ccgggtacgg ccgggtcaag
     3001 ctcaagatcg ctcccggctg ggacgtggag cccgtgcgcg ccgtgcgcgc ggcgcatcca
     3061 cagctcgacc tgcatgtcga cgcgaacggc gcgtaccccg atgacgacgc atccgccgag
     3121 gtcttccgcg tcctcgacga ggcggagctg accatgatcg agcagccttt cgcgccgcgc
     3181 gacttcgtcg cgcacgcccg attgcagcag agcatcgaga ccccgatctg cctcgacgaa
     3241 tcggtcgtcg atctcggtga tctgcgcacc atgctcgcgc tggacgccgg ccgcatcctg
     3301 aacatcaagg tgtcgcggat gggcgggctc tcggtcgcgc gcgccgcgca tgatctcgcc
     3361 gcagaggccg gtgtgccggt ctggtgcggc ggcatgcacg aatacggggt gggccgggcc
     3421 gccaacgtcg cgatctcgtc gcttcccaac ttcacgctgc cctcggatgt gtcagggtcg
     3481 gacaagtact tcacgcgcga cgtcgtgacg ccccccgtgg tggcgcacga gggacgtgtg
     3541 acggtaccca cctcacccgg gatcggtcac gacatcgacc tggaatgggt gcgccagaac
     3601 acctcccgca gcttcgacac ggacaggagt gacggatgat ccccgtcgtc gacggccaca
     3661 acgatctcgc ctgggagcgc cgcgagcgct tcggctcgac gacgacgggt ctggacgctt
     3721 cggtgccgac gctgcacacc gatctgccgc ggctcgtcgc cggcggcgtc ggcgggcagt
     3781 tctggtcggt gtgggtggac cccgaactgg tcggggccga gcaggtcacc gcgacgctgg
     3841 agcagatcga cttcgtgcag cgtcttatcg ccgcctaccc cgaccgcctc gtggccgcgc
     3901 gcacggccgc cgatgtgcgg accgcgatgg cggagggccg gatcgcctcg ctgatcggtg
     3961 tcgagggcgg tgcgcagatc gacggatcgc tcgccgtgct ccgtcagtac gcacgactcg
     4021 gcgcccggta catgacgctc acctggtcgc gcacgctcgc ctgggcggat tcggcgaccg
     4081 acgagccgca gcacggcggc ctgaccgact tcgggcgcga cgtcgtgcgc gagatgaacc
     4141 ggatcggcat gctggtggac ctggctcatg tggcgccgac gacgatgcgc gacgccctcg
     4201 ccgtgagcac gcgaccagtg gtggtgacgc attcatgcgc cctggcactg tgcgaccacc
     4261 cccgcaacgt gcccgatgac gtgctcgcga tgatcggcgc gcagggtggc gtggtgatgg
     4321 tggcgttcgt gccgtccttc gtctcgcagg cccggcgcga ctgggtgctg gcgggcgagc
     4381 acggcgagcc ccctgtggtc gggatcgcgg atgtcgccga ccatgtcgag catgtccgtg
     4441 acgtcgccgg cgtgcacgcg gtcgggctcg gcgcggatta cgacggcacc gggtcgatgc
     4501 catccggctt ggaagatgtg tcgcgctatc agagcctgct cgaggagctg cgcacccgcg
     4561 gatggtcgca gcaggatctc gaggccgtcg cgcacggcaa cgtgctccgc gtactcgagg
     4621 catccgacac cgaccacgcc gccttcttgg caggaaccgc cggcgagccg gtggcgagca
     4681 ccccgtcggt cgacatcgcg cagcgcactg ctgcgcgggc cccgcgcgtg ctcgtcgtcg
     4741 tcaacgccga gtcctcggga ccacgtcgac tcggacgctg gctcgaggag gacggcatcg
     4801 tcgtggacgc cgcgctcggg tcggacggcc tgcccgatga cctcagcggc tacgacgggc
     4861 tggtcatgct cggcggcgga ctgatgcccg acgacgacga tcgcgccccc tggctggcgc
     4921 aggagcgggt cctggcccgc gaggcgatcg acgccgatct gccgactctc ggcatctgtc
     4981 tgggcggtca gctgctcgcg cacgtcgccg gtggcgaggt acgcgcctcc ttcgggccga
     5041 aggagcgcgg cgccaccctg atcacgccgt cctcgcaggg gtcgtcggat gccctgctgt
     5101 cagcgctcgg cgacgccgtt cacatgatcg agaaccatca ggacatgatc acggcgctgc
     5161 cgccggacgc ggtgctgctc gcgtcgagcg aggccgtcga gaaccaggcc ttccgtctcg
     5221 gcgcgcacgt gcgcgggttg caattccatc ccgaggtggg cgccgaggat ctcacccggt
     5281 gggaggagcc gaccaccagg gcagagggtg accgtccact cgcgcaactc ctcgccgagg
     5341 cacgggatgt cgaccagctc aacacccgtg ccagtcgggc gatggccacg gcgttcgcgg
     5401 cagaggtacg cgctgccgca gcgacccgga tcgcaggatg acaccgcgtc cgctcgcgac
     5461 ggaggagctg gccgtgcagg cacgcgaggc gctggccgga cggctggatg ccgcgac
//
LOCUS       DATMSX010000233         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_126811,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000233 DATMSX010000000
VERSION     DATMSX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126811"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VN041_07090"
     CDS             complement(<1..887)
                     /locus_tag="VN041_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28843.1"
                     /translation="MTIRSNHRPAYAAVAVSVASIALLQNLVIPVIPMIEADLRIDSD
                     AATWTMTAWLIAAAVATPLLGRVGDLRGRRATLLAVLGLVMLGDLVAAFAPNIGVLIA
                     GRVLQGAGGAVFPLAFALLREFMPPERLTGAIGSVSALIGIGGAAGSVLAGPLSASIG
                     WRGLFEVALIAAAIGALLTVAWIPRSATRATGRLNTLSGVLLSGWLVALLLPLASGAH
                     WGWTSPATLGLFALAAVLLVAWVVSELRSATPLVDIRMLAHRSIWPVNAAGVLIGVAV
                     FGFWGYLPLFLEVPADTGWA"
     gene            1030..1599
                     /locus_tag="VN041_07095"
     CDS             1030..1599
                     /locus_tag="VN041_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28844.1"
                     /translation="MTTVAPASALAERRPKRADAARNFDALVAAGRAAFAESGSDASL
                     EDIARRAGVGIGTLYRNFPTRDDLIETLYLGEVDALAASALEVASLEPWDALRAWLDR
                     FVSYVGTKHALLDGLNRESSVLTQCRHIMLSAGEPLLSRAQQAGAVDPDITISDVVKL
                     VSGVAAVASYDSPAQRERVLNLAINAIRR"
BASE COUNT          278 a          576 c          622 g          217 t
ORIGIN      
        1 gcccagccgg tgtcggcggg cacctcgagg aacagcggca ggtagcccca gaacccgaag
       61 accgccacgc cgatcagcac gccggcggcg ttcaccggcc agatgctgcg gtgcgcgagc
      121 atccggatgt cgaccagcgg cgtcgcggag cgcagctccg agacgaccca tgccacgagc
      181 agcacggccg cgagggcgaa cagtccgagg gtggcggggc tggtccagcc ccagtgcgcc
      241 cccgaggcga gcgggaggag cagggcgacc agccagcccg acagcagcac tccggagagc
      301 gtgttcagtc gaccggtggc acgggtggcg gaccgtggga tccacgcgac cgtgaggagg
      361 gcaccgatcg cggccgcgat gagtgccacc tcgaacagac cgcgccagcc gatcgaggcc
      421 gagagcgggc cggccagcac gctgcccgcg gccccgccga tgccgatcag ggcgctcacg
      481 gagccgatgg cgccggtcag gcgctccggg ggcatgaact cacgcagcag ggcgaaggcg
      541 agtgggaaga cggcgcctcc ggcgccctgc aggacgcgcc cggcgatgag gacgccgatg
      601 ttgggcgcga atgcggcgac gagatcgccg agcatgacca ggccgaggac ggcgaggaga
      661 gtggcccgtc gtccgcgcag gtcgcccacg cgtccgagca ggggagtggc gacggccgcg
      721 gcgatcagcc aggcggtcat ggtccaggtc gcggcatcgg agtcgatgcg caggtcggcc
      781 tcgatcatgg ggatgacggg gatgacgaga ttctggagca gcgcgatcga cgcgacgctc
      841 acggcgacgg ccgcataggc gggtcggtgg ttcgagcgga tggtcatggt ccttccgatc
      901 ttgcagagag gagaggtaga atcggagagt ggctccgaac ggaggaagcc tccgcttaga
      961 agttaccgga ggatccctcc gtttcgcaag tgccgaacag acttttttcc gacatccctg
     1021 gaggtcagcg tgacgacggt ggcaccggca tctgctctgg cggagcgccg ccccaagcgg
     1081 gccgatgccg cgcgcaactt cgatgccctg gtggcggcgg ggcgcgcggc tttcgcggag
     1141 tccggatccg acgcatcgct ggaggacatc gcccgtcgcg ccggggtggg gatcggcacg
     1201 ctgtatcgca acttccccac gcgcgacgac ctcatcgaga ccctgtacct cggcgaggtc
     1261 gacgcgctgg ccgcgtcggc gctcgaagtc gcctcgctcg agccctggga cgcgctgcgc
     1321 gcctggctcg accgcttcgt ctcgtacgtg ggcaccaagc atgcgctcct cgacggcctc
     1381 aaccgcgagt cgtcggtgct gacgcagtgc cgtcacatca tgctctcggc gggggagccg
     1441 ctgctctccc gcgcgcagca ggccggcgcc gtcgatcccg acatcaccat cagtgacgtc
     1501 gtgaagctcg tctccggcgt cgcggccgtg gcctcctacg acagcccggc ccagcgcgag
     1561 cgcgtcctga acctcgcgat caacgccatc cggcgctgac atcgcccgcg cggcgtcgcg
     1621 cgtccggatg cagaacgggg gccgacacgc cactctccgg acgtctccag cgagaacgtc
     1681 cgagatgtgg cgt
//
LOCUS       DATMSX010000234         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_174033,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000234 DATMSX010000000
VERSION     DATMSX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174033"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VN041_07100"
     CDS             complement(<1..429)
                     /locus_tag="VN041_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWU28845.1"
                     /translation="MSVRQSLLAILDQGPCYGYQLRAEFDRRTGSTWPLNVGQIYNTL
                     ERLERDGLVRKGDVDDQGHVYYSITDAGRDEVRGWLDSPVSQQGGTRDELPIKLALAA
                     TLPGVDVAAVMRRQRETSLAVLEGLRAARPPSATGGEDLDR"
     gene            641..>2164
                     /gene="secA"
                     /locus_tag="VN041_07105"
     CDS             641..>2164
                     /gene="secA"
                     /locus_tag="VN041_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWU28846.1"
                     /translation="MANPLEKMLRAGEGRILRRLKQTVKAVNALEDDFEKLTDEELRG
                     ETAELRARYEKGETLDQLLPEAFAAVREAARRTLGMRAYDVQIMGGAALHNGNIAEMK
                     TGEGKTLVAAFPSYLNAIAGKGVHVITVNDFLASYQSELMGRVHRALGMTTGTIISGQ
                     TPDVRRHQYAADITYGTNNEFGFDYLRDNMAWRKEDLVQREHFFAIVDEVDSILIDEA
                     RTPLIISGPSSGEANRWFTEFAKIARTLEPGVDYEVDEKKRTVGVLEPGIEKVEDYLG
                     IDNLYESANTPLISFLNNAIKANALFKKDKDYVVMNGEVLIVDEHTGRILMGRRYNEG
                     IHQAIEAKEGVEVKAENQTLATVTLQNYFRLYKKLSGMTGTAETEAAEFMSTYKIGVV
                     PIPTNKPMIRIDQPDLVYKNEEAKFGQVVEDLVRRHEKGQPVLVGTTSVEKSEYLSRL
                     LAKKGVRHEVLNAKNHAREAAIVAQAGRLGSVTVATNMAGRGTDIMLGGNAEFLAVAE
                     "
BASE COUNT          428 a          737 c          678 g          321 t
ORIGIN      
        1 ccgatctagg tcctcgccgc cggtcgcgct cggcggtcgg gccgcacgca gcccttcgag
       61 aaccgcgagc gacgtctcac gctgacggcg catcactgcc gcgacgtcca ctccggggag
      121 ggtcgccgcc agcgcgagct tgatcggcag ctcgtcgcgc gtgccgccct gctgcgaaac
      181 gggggagtcc agccagcccc gcacctcgtc gcgaccggca tccgtgatcg agtaatagac
      241 gtgcccctgg tcgtcgacgt cgcccttccg caccagtccg tcccgctcca gccgctcgag
      301 ggtgttgtag atctgaccga cgttgagcgg ccaggtcgac ccggtgcggc ggtcgaactc
      361 ggcgcgcagc tggtatccgt agcacggacc ctgatcgagg atggcgagca ggctctggcg
      421 gacggacatg cggtctccga tcatctcccg ggtattgcat tgccgagtat atgcatgccc
      481 ggaaacgtcg caccgacgcc gtgatccgcg tgtcctgttc gcgctcagcg tggccggccg
      541 gccccgcagt gcacagcgga tgcatgtcac gggccggtaa catgacacgg tatgcccggc
      601 gacgaccctt gccggcaccc gagatccaag ggagacatcc gtggcgaatc ctctcgagaa
      661 gatgctgcgc gccggcgaag gacgcatcct tcgcaggctc aagcagaccg tcaaggccgt
      721 gaacgcgctg gaggacgact tcgagaagct caccgatgag gagctgcgcg gcgagaccgc
      781 cgagctgcgg gcacgctacg agaagggcga gaccctcgac cagctcctgc cggaggcgtt
      841 cgccgccgtc cgcgaggccg cgcgccgcac cctcggcatg cgcgcctacg acgtgcagat
      901 catgggtggc gccgccctgc acaacggaaa catcgccgag atgaagaccg gtgagggcaa
      961 gacgctcgtc gcggccttcc cgtcctacct caacgccatc gcaggcaagg gcgtgcacgt
     1021 catcacggtc aacgacttcc tggcctcgta ccagtccgag ctgatggggc gcgtccaccg
     1081 cgccctcggc atgaccaccg gcacgatcat ctccggtcag actcccgacg tgcgccgcca
     1141 tcagtacgcc gcggacatca cctacggcac gaacaacgag ttcggcttcg actacctgcg
     1201 cgacaacatg gcatggcgca aggaggacct cgtccagcgc gagcacttct tcgccatcgt
     1261 cgacgaggtc gactccatcc tcatcgacga ggcgcgtacg ccgctgatca tctccggccc
     1321 ctcctccggc gaggcgaacc gctggttcac cgagttcgcg aagatcgcgc gcaccctcga
     1381 gcccggcgtg gactacgagg tcgacgagaa gaagcgcacg gtcggcgtgc tcgagcccgg
     1441 catcgagaag gtcgaggact acctcggcat cgacaacctg tacgagtcgg cgaacacacc
     1501 gctgatctcc ttcctgaaca acgccatcaa ggcgaacgcc ctgttcaaga aagacaagga
     1561 ctacgtcgtc atgaacggcg aagtgctcat cgtcgacgag cacaccggcc gcatcctgat
     1621 ggggcgccga tacaacgagg gcatccacca ggccatcgag gcgaaggagg gcgtcgaggt
     1681 caaggccgag aaccagaccc tcgccacggt gaccctgcag aattacttcc gcctctacaa
     1741 gaagctgtcc ggcatgaccg gtacggcaga gaccgaagcc gctgagttca tgtcgacata
     1801 caagatcggt gttgtgccga tcccgacgaa caagccgatg atccgcatcg accagccaga
     1861 cctcgtttac aagaacgaag aggccaagtt cggccaggtc gtcgaagatc tcgttcgtcg
     1921 ccacgagaag ggtcagcccg ttctcgttgg aaccacgagc gttgagaaga gcgaatacct
     1981 ctcacggttg ctcgccaaga aaggcgtgcg gcacgaggtg ctgaacgcga agaaccacgc
     2041 gcgtgaggcg gcgatcgtcg cccaggccgg gcgactcgga tccgtcacgg ttgccaccaa
     2101 catggcaggc cgcggaacag acatcatgct cggaggcaac gccgagttcc tcgccgtcgc
     2161 cgag
//
LOCUS       DATMSX010000235         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_164296,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000235 DATMSX010000000
VERSION     DATMSX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164296"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1389
                     /locus_tag="VN041_07110"
     CDS             235..1389
                     /locus_tag="VN041_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU28847.1"
                     /translation="MANVREIGIIMNGVSGRMGYRQHLVRSILAIRDQGGIELSDGSK
                     VTVKPILVGRSEEKLAELAAKHGIADYTTDLDAALADPQWEIYADFLVTKARATAIRK
                     AIAAGKAIYTEKPTAESLEEAIELAQLAKTAGVKTGVVHDKLYLPGLQKLKRLIDSGF
                     FGRILSVRGEFGYWVFEGDWQPAQRPSWNYRTEDGGGIITDMFPHWNYVLENLFGEVK
                     SVYAQAAVHIADRWDEKGEHYDATAEDAAYGIFELEGGIIAEINSSWTVRVNRDELVE
                     FQVDGTHGSAVVGLFGCKIQPRNATPKPVWNPDLEDTHDYDADWQTVPTNDVFLNGFR
                     QQWEEYLESYVLGTDYGFDLLAGARGVQFAEAGLASSAEGRKIAIEKLSL"
     gene            1386..2555
                     /locus_tag="VN041_07115"
     CDS             1386..2555
                     /locus_tag="VN041_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWU28848.1"
                     /translation="MTALRLLSASGAVTDAELNEVTGFTRPTAPLRSRVAYAAAHVVP
                     VAWGDNTPGQPADIDWDSTLAFRRNVYSWGLGVADAMDTAQRNMGLDAAATRELISRS
                     AQVAREEGGSVVVGVNTDHVDETHISLDQVIDAYKSQLHFTEEQGAGPVLMASRHLAR
                     VAESAEDYRRVYRSVLESATTPVVLHWLGTAFDPELEGYFGASDWQTASGVLLDIIAE
                     NPGKVAGVKMSLLNAESEISVRERLPEGVRMFTGDDFNYVSLIGDSGTGARPYSDALL
                     GAFAAITPVASAAIQALDAGDAAAYQRILGPTEELSRQVFAAPTFYYKTGVAFLAWLN
                     GHQPAFQMVGGLHSARSLPHLSRIVELANASLALENPELARERWHGMLRLNGVTL"
     gene            2552..>2859
                     /locus_tag="VN041_07120"
     CDS             2552..>2859
                     /locus_tag="VN041_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWU28849.1"
                     /translation="MSAVDPRLSINQATIKYADLATALKVTADAGVQSIGLWREPVNE
                     VGLDVACRMLNDSGLRFSTHCRGGFFTLPDGPERTAALEDNRRAIDETAALAAAGAAG
                     S"
BASE COUNT          469 a         1019 c          958 g          413 t
ORIGIN      
        1 ggcgagggag cgcgtgaccg cggcccgcgc ggcctatgag gagcggatcg gtgcgcgcct
       61 gcgggcctgg tcggcgggcg acctcaatca cttcgccgag ctgctggacg cgctcgggcg
      121 ctgacgggcg gaacccggcc cttgcgctcc acgtgacaac ctgctttgat atgggaaagc
      181 gcttaccgag gtagccggtg gcgcgatgac ttcccgaaca gaacggaaac accaatggcg
      241 aacgtgcgcg agatcggcat catcatgaac ggcgtctccg gacgcatggg ctaccggcag
      301 cacctggtgc gctcgatcct cgcgatccgc gaccagggcg gcatcgagct gtccgacggg
      361 tcgaaggtga ccgtcaagcc gatcctcgtc ggccgcagcg aggagaagct cgccgagctc
      421 gccgccaagc acggcatcgc cgactacacc accgacctcg acgcggccct cgccgacccg
      481 cagtgggaga tctacgccga cttcctggtc accaaggcgc gcgccaccgc catccgcaag
      541 gccatcgccg cgggcaaggc gatctacacc gagaagccca ccgccgagtc gctggaggag
      601 gcgatcgagc tcgcgcagct ggcgaagacc gccggtgtga agaccggcgt cgtgcacgac
      661 aagctgtacc tccccggcct gcagaagctc aagcgcctca tcgactcggg cttcttcggc
      721 cgtatcctct ccgtgcgcgg cgagttcggc tactgggtgt tcgagggtga ctggcagccc
      781 gcgcagcgcc cgagctggaa ctaccgcacc gaagacggcg gcggcatcat caccgacatg
      841 ttcccgcact ggaactacgt gctcgagaac ctgttcggcg aggtcaagag cgtctacgcc
      901 caggccgccg tgcacatcgc cgaccgctgg gacgagaagg gcgagcacta cgacgccacc
      961 gccgaggacg ccgcgtacgg catcttcgag ctggagggcg gcatcatcgc cgagatcaac
     1021 tcctcctgga ctgtgcgcgt gaaccgcgac gagctcgttg agttccaggt cgacggcacg
     1081 cacggatcgg ccgtggtcgg cctgttcggc tgcaagatcc agccgcgcaa cgccaccccg
     1141 aagccggtgt ggaaccccga cctcgaggac acccacgact acgacgccga ctggcagacc
     1201 gtccccacca atgacgtctt cctgaacggc ttccgccagc agtgggagga gtacctggag
     1261 tcatacgtgc tcggcaccga ctacggcttc gacctgctgg ccggcgcccg cggcgtgcag
     1321 ttcgccgagg ccggcctcgc ctccagcgcc gagggccgca agatcgcgat cgagaagctg
     1381 tcgctgtgac cgcgctgcgc ctgctgtcgg catccggagc agtgacggat gccgagctga
     1441 acgaggtcac cggattcacc cgccccaccg cgccgctgcg ctcgcgcgtc gcctatgccg
     1501 cggcgcacgt cgtgccggtc gcctggggcg acaacacccc cggtcagccc gccgacatcg
     1561 actgggactc gacgctcgcg ttccgtcgga acgtctactc ctgggggctc ggtgtcgcgg
     1621 acgccatgga caccgcgcag cggaacatgg gcctcgacgc cgctgcgacg cgcgaactca
     1681 tctcccgcag cgctcaggtg gcgcgcgagg agggcggctc cgtcgtggtc ggcgtcaaca
     1741 ccgaccatgt ggacgagacg cacatctccc tcgaccaggt gatcgacgcg tacaagtcgc
     1801 agctgcactt caccgaggag cagggggcag ggccggtgct gatggcatcc cggcacctcg
     1861 cccgcgtcgc ggagagcgcc gaagactacc gccgggtgta ccggtccgtt ctggagagcg
     1921 ccaccactcc cgtcgtgctg cactggctcg gcactgcgtt cgatcctgag ctcgagggct
     1981 acttcggcgc atccgactgg cagacggcat ccggcgtgct gctggacatc atcgccgaga
     2041 acccgggcaa ggtcgcgggc gtgaagatga gcctgctgaa cgccgagagc gagatctcgg
     2101 tgcgcgagcg ccttcccgag ggtgtgcgga tgttcaccgg cgacgacttc aactacgtct
     2161 cgctcatcgg cgactccggc accggggctc ggccgtactc ggacgcgctg ctcggcgcct
     2221 tcgcggcgat cacgccggtg gcatccgccg cgatccaggc gctcgatgcc ggcgatgcgg
     2281 cggcgtacca gcgcatcctc ggcccgacgg aggagctcag ccgtcaggtg ttcgccgcgc
     2341 ccaccttcta ttacaagacc ggcgtcgcat tcctggcctg gctgaacggc caccagcccg
     2401 cgttccagat ggtcggcggc ctgcactccg cgcgcagcct gccgcacctg tcgcggatcg
     2461 tcgagctggc caacgcctcg ctcgccctgg agaacccgga gctcgcgcgc gagcgctggc
     2521 acgggatgct gcgcctgaac ggagtcactc tgtgagtgct gtcgaccccc gtctgtcgat
     2581 caaccaggcg accatcaagt acgccgacct cgcgacggcg ctgaaggtga ccgccgacgc
     2641 cggcgtgcag tcgatcggac tctggcgcga gccggtgaac gaggtcgggc tcgacgtcgc
     2701 gtgccggatg ctgaacgact cgggactgcg cttctcgacg cactgccgcg gcgggttctt
     2761 caccctgccc gacgggcccg agcggaccgc cgcgctcgag gacaaccgcc gggcgatcga
     2821 cgagaccgcc gcgctcgccg ccgcaggagc cgctggttc
//
LOCUS       DATMSX010000236        18254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_155173,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000236 DATMSX010000000
VERSION     DATMSX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 18254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18254
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155173"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VN041_07125"
     CDS             <1..215
                     /locus_tag="VN041_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase"
                     /protein_id="HWU28850.1"
                     /translation="AGELAGSDAAAVGEALRKRDASDSSVVDFLNAAPGVTVVDSTHL
                     DFTQTIDAVLAVIERAFDAEKEPHHG"
     gene            208..1728
                     /gene="der"
                     /locus_tag="VN041_07130"
     CDS             208..1728
                     /gene="der"
                     /locus_tag="VN041_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWU28851.1"
                     /translation="MADEEYEGGPDHLAEKMAELDEQLADQRADLMRATLADYDLDEE
                     DAHLLEGVTLGEDGITVMPALPVVAIVGRPNVGKSALVNRILGRREAVVEDTPGVTRD
                     RVTYKAEWMERRFSLVDTGGWEPDAKGIDRSVAAQAEVAIDLADMVLFVVDAKVGATS
                     TDEHVVKLLRKSGKPVFLIANKIDDARQEPEAAALWNLGLGEPYPVSAIHGRGVADLL
                     DMIVEKLPEVSAVAKAEIGGPRRVAILGRPNVGKSSLLNKAAKEERVVVNDLAGTTRD
                     PVDEIVELGGKMWRLVDTAGIRRRVHLQQGADFYASLRTSAALEKAEVAIVVLDVSQP
                     ISVQDLNIIDMVLESGRALVLAFNKWDRLSDDDMENSDRRRYLEREIEQDLAHVAWAP
                     RVNISAKTGWHFDKLVPALERALENWDRRIPTGKFNAFITELVAAHPHPLRGGKQPRI
                     LFGTQASTRPPTFVLFTTGFLDPGYRRFVQRRLRELYSFEGTPIVINMRVRERRQR"
     gene            1849..2919
                     /locus_tag="VN041_07135"
     CDS             1849..2919
                     /locus_tag="VN041_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HWU28852.1"
                     /translation="MTHTLPLPDFAHERVEVTTGRRSGLFIAVALHSSVLGSALGGAR
                     LWTYPHWSDALGDALRLSAAMTLKNAAAGLDAGGGKSVIRLEPGQTLDADQRRAAFLD
                     LGDAVELLDGRYRTAEDVGSTTEDMLTVSERTQHVVGLPSTVGGSGEPAGPTSLGVYA
                     SLQAVLERITGSASADGRRVTISGLGQVGGRLAARLAEQGAQLTVTDINPARRSFAEE
                     IGATWVEPGTEHLVPGDVFVPAGIGGVLTDEVIDVLDVKAVCGPANNPLADRSGADRL
                     AGRGILYAPDFVVNAGGVIYLDLEAKQLGTSDEIMARVAAIGDTVRTILDDAEERGVT
                     PLQAAEELAASRLRAGAAVGAR"
     gene            2949..3401
                     /locus_tag="VN041_07140"
     CDS             2949..3401
                     /locus_tag="VN041_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28853.1"
                     /translation="MGDGTQHEVEVLTSVSDEDAADLRHLLGLLSTTAVFDRARIESM
                     ITHDATELLVVREQGRIVGMTTIVTFPLPTGIRGIVEDVAVHESMRGKGIARLLLERA
                     TLLARERGLRTLDLTSRPSREAALRLYESVGFVRRDTNVLRYTPDAVS"
     gene            3454..4359
                     /locus_tag="VN041_07145"
     CDS             3454..4359
                     /locus_tag="VN041_07145"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWU28854.1"
                     /translation="MTIIPVQPGEPRRPDGPRDPGDAWVVAESGERYWGRFGAAGVLA
                     FDPERGVLLQHRVGWSHFGGTWGIPGGALHEGESARDGALREAQEEAGIPDGALKARF
                     ASVLDLRIWSYTTVVADVVSSFEPVISDPESLALEWVPVDEVDQRPLHPGFGAAWPAL
                     RALLSARPAVVVDAANVVGSVPDGWWKDRVGAAERLRERLSTWTGGGVTAPGLGVGAD
                     LWYPPVSMVVEGEAREIADAVDERVEIVRASGAGDDAIVSETRRLRDGGFSVTAVTSD
                     RGLRARLEEAGAQVQSSGWLLERLG"
     gene            complement(4418..4792)
                     /locus_tag="VN041_07150"
     CDS             complement(4418..4792)
                     /locus_tag="VN041_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWU28855.1"
                     /translation="MTDAVKPARIDSWLWAVRVYKTRSAATTAVRAGHVRVNGERIKA
                     AQTVKPGDEVRVRISGFDRILIVRQTLTKRVGAPVAATAYDDRTPPRDPVAMLAVRDR
                     GAGRPTKRERREIDRLRGRNDL"
     gene            complement(4789..5457)
                     /locus_tag="VN041_07155"
     CDS             complement(4789..5457)
                     /locus_tag="VN041_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HWU28856.1"
                     /translation="MPRSIRLLDVLAWVPYIVLSIIHVSAIALDSPLAPPTKLWLMPL
                     LALPVAAAAPRLRPRAVVGLLLAALLFSWLGDSAGAFFPTLPELPLMLGFFGIAHIGY
                     IVLFLRFARIRRLPWWTLVYIAWWVGMLSILGPHTGGLFPAVAAYGLVLAGTAVTAAR
                     CHPIVTIGGAFFLASDSILAFRLFLPEDLPGWMSPTVMITYTIGQGLIVWGVLHTLAR
                     RETR"
     gene            complement(5496..5951)
                     /locus_tag="VN041_07160"
     CDS             complement(5496..5951)
                     /locus_tag="VN041_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28857.1"
                     /translation="MSAQHPSIDDPELGVLTRAESTFDDGTVAVHDWYAGSVADGDAD
                     IELMVDGPEVDDVRSRLPRLRSVVADIDGIRRRASDAVISHFSDVEPEAHELDEGAAD
                     LRLEAIEASSDDEIVLHFVDTCGQHFPEGYWPAAHLAADDTVAAVTVES"
     gene            complement(5974..6555)
                     /locus_tag="VN041_07165"
     CDS             complement(5974..6555)
                     /locus_tag="VN041_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28858.1"
                     /translation="MTSITTERATISRWDDVQHSLTGGGDGRSCQCIWPVVSNKEWQS
                     TTVEQRRDLLKDEIAAGPPPGLIAYVDGEAAGWIRIGPRSAQARIVRTRAIVAATAEP
                     LDDASVWAVTCFVIRRDHRGKGLSAQLLKAAIEYARESGARLIEAYPVDTSEGTHHSN
                     DLFHGTLSTFLAHGFRQTKPLTRGRVLVVRELE"
     gene            6686..6759
                     /locus_tag="VN041_07170"
     tRNA            6686..6759
                     /locus_tag="VN041_07170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6720..6722,aa:Pro,seq:ggg)
     gene            complement(7730..8239)
                     /locus_tag="VN041_07175"
     CDS             complement(7730..8239)
                     /locus_tag="VN041_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28859.1"
                     /translation="MDPTALAFALIAALGGAALTAVAASIGAIWQSKREHKKWLRERR
                     FDAYKEFVPAMRGLDLHFAAITSVKHDLDALNDARPAVPDASYTGRVDRLRLRLDAAT
                     TDEKPFTARALDSMAAVTILGPTSVTNHVESYFVAIRVQDNDAITAAEDKLLAAMRKA
                     VRVTRWSRG"
     gene            8961..11627
                     /locus_tag="VN041_07180"
     CDS             8961..11627
                     /locus_tag="VN041_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWU28860.1"
                     /translation="MSTLSIALALRFGLESRRDDIDRAVELSALAVALARQLDDARLD
                     ERCLRAGATLADRFESQGDDQDLVTAVEYFREALAATEPLGGTVSGRCADLARALLIK
                     YGRWARVEDLDEAATLYRRAIDELPAGHYEIASHKSGLGGVLVTRYEHYGDSADLAPA
                     IELLTEAYELTDSADPAKPIRLGRLAEALIQSHSHFGAIADLDNAIRLLEFGLEHPSP
                     NTRIHVSTQVTLAGALRRRGLLRNDIGDLGRTELLLQEVAARSERAAYGDASLTFNLA
                     SVRSDMHSISGEASHLSSAIAGMREALAVSRVDSPLHSHAKTFLAQLLLIEADDAGVA
                     SDSPVFAECLALSREAAVDPILVPALGLISAATWAELADGTERLVAYDRALAILPYVS
                     SHGLTVADSLSRLRDLPSVASDACAAFVEAGQLERAVEALEEGRAIAVRLRMRRRTDV
                     EELRRVRADLAEAYEDALEGTNHPTSGLDVDPRGGSRRLEELLAQIREITGFARFGFP
                     RTFREIQALIGDAMVVYLNTSSRRCDALVVTATGVTVVSLSTSAAEIAGRATDFLSAM
                     EESVVARAVSEVVRSERVGRDVAAWLWDAVVHPVLDELQLDPQEETTDATLWPRVWWI
                     PTGHLAVLPIQSAGHHAEYGSQVITSSHTAIDRVVSSQIPSIETLAAARQVSDDGADD
                     IRCLIVASSHPAGESILRGVDEEVERLVAQLGRERTTLLADQASLLPDGPSTKVAVLS
                     MLAQNNWAHFACHASPSPENPTESQLILTDHRADPLRVEDLLTVRPIAPRLAYFSACS
                     TAQISPDLAHEPVHFGIAAALAGYAHSVLTLWPVRDDPEIADAVYKGVLRAMSWPMDA
                     STGAAVHDANRMMRKRSANAIHRWAATIHQGS"
     gene            12036..12458
                     /locus_tag="VN041_07185"
     CDS             12036..12458
                     /locus_tag="VN041_07185"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWU28861.1"
                     /translation="METNAPEGWYEDGSGSQLRYWDGARWTDEFAPRLPAPASTSVVP
                     SPGSDQKRERTGGPVLGIVGIVAAVLGLFGVVLPWIAAETAGILLLIAAFALSGIAAA
                     RAKRRWIGAIGLGIAAVGTAAGFVMFAIGLAQSLGAFR"
     gene            12533..13249
                     /locus_tag="VN041_07190"
     CDS             12533..13249
                     /locus_tag="VN041_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28862.1"
                     /translation="MEPNGTEAPVGAPGKSGKNVVNRRPRVGEIADTLRERIISGDLQ
                     PGERLVEQTLANDLRVSRMPIREALNILRGQGFVSAIPNRGMVVRPLSDQDIENLFEV
                     REVLDQLTARRAAERATDWEIEQMRLALEGGTTSLGADATDAHGRINQGFHDVLTAAA
                     HNDLLVTINEPIEGRLQWLVSRFDHPATYHQEHLAIFDAIRRRDPDAAAAAALAHLQT
                     SRELWRSSQARDRAQIARTR"
     gene            13416..14801
                     /locus_tag="VN041_07195"
     CDS             13416..14801
                     /locus_tag="VN041_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28863.1"
                     /translation="MVQQNSSSLIERMDRLPVNRAHWKIAMIGGLGLLFDGMDGSLVS
                     YILPILRDKWALDGLQSGLIGSSLLIGMLIGALTAGPISDRIGRRRVMMWALLLFTLA
                     TFVAAFSMNWQMFFLLRVLAGIGIGAESAVIPTYVAELIPAKRRGLFAGAVAGFLAFG
                     YVLASLIGYFVVARFDDGWRYGQIITAIPVLLVLWWRRAMPESPRWLLSRGENDKAEE
                     IVGGIERGVLVPVTSPLSWKAQGDGTNSPAKLIQHHELLGRDLLGRTLLLWLIWMALT
                     FSFFGFFSWIPSLLVDRGLTVTTSFSYVLLMTIAQIPGYYGAAWLNEKIGRKTNIALF
                     LAIGTAGAWLLSQSVDPWQVIASGALLSAGMNGTYAALYAYTPEVYPTRLRATGMGMA
                     SAVGRIGGIAAPIIIGVSQQALGFAGVFIMIMSVLAVTAVLVVVFGVSTKGRSLESLT
                     EAVPIVAQKEN"
     gene            14807..15997
                     /locus_tag="VN041_07200"
     CDS             14807..15997
                     /locus_tag="VN041_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU28864.1"
                     /translation="MPIRSDSPRPAAPIALVGARVLDTDANVLSGPAVVVLRDGLIDE
                     VREGAASAPAGTDAVDVTGRVLMPGLIDAHVHVTQAGTHAEIHDWDSSYHVARSAREM
                     EHMLRRGFTTVRDMGGAETGLARAVEERLFTGPRLLAGGPIFSPTGGHGLTHIVDGEV
                     ELRKALRAQFRDGADHIKLTVSGGVIAKMRIEALGFSEEELRAAVDEARLAKRYIGAH
                     AYTAEAVNRALRLGVRTIEHGTYLDDESIRLLTELEDVHLVPTLVALWGMLQGESRGT
                     IGDDARQSVQDLFEVGLQTLKTADSAGVSIGFGTDLHGSGQRLQLEEIRIRARVQTPE
                     AILRSLTTVGADIVRRADRIGRVAPGYDADLLLVDTDPRVDLDAVADATRSRLVITAG
                     EIVD"
     gene            complement(16007..16696)
                     /locus_tag="VN041_07205"
     CDS             complement(16007..16696)
                     /locus_tag="VN041_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWU28865.1"
                     /translation="MDRIRTLGIIGAGRLGSVLAALAASAGYGVLVARSGDPAPISRA
                     MTALGAQAVTVERMATEADGVVLALPLGAYRTLPADLLRGMLVIDAMNYWWGADGIRP
                     DLTDPRTSTSELVQAHLSGARVVKALGHMGYQDLEDDARPAGDPDRRAIAICGDAADD
                     LALVSQLVDAVGFDPVIAGPLASGIMLEPGAEAFGADVDAAELRAMLDRFPTSQRGIV
                     VARARGGALWD"
     gene            16833..17546
                     /locus_tag="VN041_07210"
     CDS             16833..17546
                     /locus_tag="VN041_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28866.1"
                     /translation="MSSRRTRVLSAWAVHAFTLTGVLWATLALLALFDDDPKLMWLYL
                     GIALVVDGVDGTLARKAEVRTYAPHFDGVILDSIIDYLTWTLIPALFLFRTGLLGEGA
                     LAVVLLLLITVSSMFCYANTKMKTGDYYFLGFPAAWNVVALAIWLLDAGIVVNAIIVV
                     VLALLTWAPIAFVHPLRVARFRTLNIIATAVWILASAALVIAYPTAEPWIAVVWLVSG
                     AWVILTGIIRTVAATRALR"
     gene            complement(17438..>18254)
                     /locus_tag="VN041_07215"
     CDS             complement(17438..>18254)
                     /locus_tag="VN041_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28867.1"
                     /translation="VEPAQLRDPVQRVVVLREALEHGIRMLFVEVASPLEGVLLADDL
                     PGLVHPVDDAPQEEDALHAVNVVQLLRFCLASAPVETAEALTGMPTRHVPRAAMRLLP
                     PDAPVRRMHPLRRLMGDPSVPVYLAVGIYSALRALPVAFIPQFHGSLLVLWMIDILTA
                     VPYTWGVLAMLFAPRRWIRMLATLTTITTFAAPYVYFWMHGRDYPPFVVAVIAALTIA
                     SISIEVVKYLQERMLRRRYRSARVAATVRMIPVRITQAPETSQTTAIQGSAVG"
BASE COUNT         2900 a         6048 c         6305 g         3001 t
ORIGIN      
        1 gtgcgggcga gctcgccggc tcggacgcgg ccgccgtcgg cgaggccctg cgcaagcgcg
       61 acgcctccga cagtagcgtc gtcgacttcc tcaacgccgc acccggcgtg acggtggtcg
      121 actccacgca cctggatttc acacagacca tcgatgccgt gctcgctgtg atcgagcgcg
      181 ccttcgatgc cgaaaaggag ccccaccatg gctgatgaag agtacgaggg cggccccgac
      241 cacctcgccg agaagatggc cgagctcgac gagcagctgg ccgaccagcg cgccgacctc
      301 atgcgcgcga ccctcgccga ctacgacctg gacgaggagg acgcgcacct gctcgagggc
      361 gtgaccctcg gcgaggacgg catcacggtc atgccggcgc tgccggtcgt ggcgatcgtg
      421 ggccgcccga acgtgggcaa gtcggcgctg gtgaaccgca ttctgggccg ccgcgaggcc
      481 gtcgtggagg acacccccgg ggtcacgcgc gaccgcgtca cctacaaggc cgagtggatg
      541 gagcggcgct tctcgctcgt ggacaccggc gggtgggagc ccgatgccaa gggcatcgac
      601 cgctccgtgg ccgcgcaggc cgaggtcgcg atcgacctgg ccgacatggt gctgttcgtc
      661 gtcgacgcga aggtcggcgc cacctccacg gacgagcacg tcgtcaagct gctgcgcaag
      721 agcggcaagc cggtgttcct catcgcgaac aagatcgacg atgcccgtca ggagcccgaa
      781 gccgctgcgc tgtggaacct gggtctgggg gagccgtacc ccgtctccgc catccacgga
      841 cgcggcgtcg ccgacctgct ggacatgatc gtcgagaagc tgccggaggt ctcggcggtg
      901 gccaaggccg agatcggcgg cccgcgccgc gtggcgatcc tgggcaggcc gaacgtgggc
      961 aagtcctcgc tgctgaacaa ggcggccaaa gaggagcgcg tggtcgtcaa cgacctcgcc
     1021 ggcaccacac gcgacccggt ggacgagatc gtcgagctgg gcggcaagat gtggcggctg
     1081 gtcgacaccg ccggtatccg ccgccgtgtg cacctgcagc agggcgccga cttctacgcc
     1141 tcgctgcgca cctccgccgc gctggagaag gccgaggtcg cgatcgtcgt cctcgacgtg
     1201 agccagccca tcagcgttca ggacctcaac atcatcgaca tggtgctgga gtcgggccgt
     1261 gcactggtgc tcgcgttcaa caagtgggat cgcctgtcgg acgacgacat ggagaactcc
     1321 gaccgccgcc gctacctgga gcgcgagatc gagcaggacc tggcgcacgt cgcgtgggct
     1381 ccgcgcgtga acatctcggc gaagaccgga tggcacttcg acaagctcgt gcccgcactg
     1441 gagcgcgcgc tggagaactg ggaccgtcgt atcccgaccg gcaagttcaa cgcgttcatc
     1501 accgagctcg tggccgcgca tccgcacccg ctgcgcggtg gcaagcagcc gcgcatcctg
     1561 ttcggcaccc aggcgtcgac ccgtccgccg acattcgtgc tgttcacgac gggcttcctc
     1621 gacccgggct accgccgatt cgtgcagcgc cgtctgcgcg agctgtactc gttcgaaggc
     1681 acgccgatcg tgatcaacat gcgcgtgcgg gagcggcgcc agcgctagcg ctgccgccgg
     1741 gtctcccggc tgggtccctg agttcgtcga agggataatg gggagcggat gccatgatcc
     1801 cggcatccgt tcccgcctgg cccacgaggc ctgccgaagg agactgcgat gacgcacacc
     1861 ctgcccctgc ccgacttcgc ccacgagcgc gtggaggtga cgacgggacg gcgcagcggc
     1921 ctcttcatcg ccgtcgcgct gcactcctcg gtgctcggct ccgccctggg cggggcgcgg
     1981 ctgtggacct acccgcactg gagcgacgcc ctcggcgacg ccctgcgcct ttccgccgcg
     2041 atgacactga agaacgcggc ggccggtctg gatgcgggtg gcggcaagtc cgtcatccgt
     2101 ctggagccgg ggcagaccct ggatgctgat cagcgtcgcg ccgcgttcct cgatctcggg
     2161 gatgccgtgg agcttctcga cggtcgctat cgcacggcgg aggatgtggg ctcgacgacc
     2221 gaggacatgc tcacggtgag cgagcgcacg cagcatgtgg tgggcctgcc gtcgactgtc
     2281 ggcggttccg gggaacccgc gggtcccacc agcctggggg tgtacgcctc gctgcaggcg
     2341 gtcctggagc ggatcaccgg atcggcttcc gccgacgggc gccgggtgac gatctcgggc
     2401 ctcgggcagg tcggcggacg acttgccgcg cgcctggcgg agcagggcgc acagctgacc
     2461 gtgaccgaca tcaaccccgc gcggcgctcg ttcgccgagg agatcggtgc gacctgggtc
     2521 gagccgggca ccgagcacct ggttcccggt gacgtgttcg ttcccgccgg catcggcggc
     2581 gtgctgaccg acgaggtcat cgacgtactg gacgtgaagg ccgtgtgcgg ccccgcgaac
     2641 aacccgctgg ccgaccgctc cggagcggat cgcctcgccg ggcgcggcat cctgtacgcg
     2701 cccgacttcg tcgtcaacgc gggcggcgtg atctacctcg acctggaggc gaagcagctc
     2761 ggcacgagcg acgagatcat ggcccgcgtc gccgcgatcg gcgacaccgt gcgcaccatc
     2821 ctggacgacg ccgaggagcg gggcgtcacg ccgctgcagg cggccgagga gctcgccgcg
     2881 tcgcggctgc gtgccggtgc cgcggtgggg gcgcggtaac gcgagaaccg attgacgaga
     2941 ggacgacgat gggcgacggc acgcagcacg aggtggaggt tctcacctca gtcagcgatg
     3001 aggacgccgc cgacctgcgg catctgctcg gactgctctc cacgaccgcc gtgttcgatc
     3061 gggctcgcat cgagtcgatg atcacccacg acgcgacgga gctgctggtc gtccgcgagc
     3121 agggccggat cgtcggcatg acgacgatcg tcacgttccc gctcccgacg ggcatccgcg
     3181 ggatcgtcga agacgtcgcc gttcacgagt cgatgcgcgg gaaggggatc gcccgtctgc
     3241 tgctggagcg ggcgaccctt ctcgcgcgcg aacgcgggct gaggacgctg gatctgacct
     3301 cacgcccctc gcgcgaagcg gcgctgcggc tgtacgagtc ggtcgggttc gtccgccgcg
     3361 acacgaacgt gctgcggtac acgccggatg ccgtgagctg actcacttcg tcttcgtggc
     3421 cgctgcgctg cgcagctgtg aaaggctgaa cgggtgacga tcattcctgt gcaacccggt
     3481 gagccgcgcc gtccggacgg accgcgggac ccgggggatg catgggtcgt cgccgagtcg
     3541 ggcgagcggt actggggtcg attcggcgcc gccggcgtgc tcgcgttcga ccccgagcgc
     3601 ggcgtgctgc tgcagcatcg ggtgggctgg agccacttcg gcggcacctg gggcattccc
     3661 ggaggcgcgc tgcatgaggg cgagtccgcg cgtgacggcg cgctgcgcga ggcccaggag
     3721 gaggccggga tccccgacgg cgccctgaag gcacggttcg cgagtgtgct ggatctgcgg
     3781 atctggtcgt acacgaccgt ggtcgccgac gtggtgtcgt cgttcgagcc ggtgatcagc
     3841 gaccccgaga gcctcgcgct cgagtgggtg ccggtggacg aggtggacca gcgtccgctg
     3901 catccggggt tcggcgcggc ctggcctgct ctgcgcgccc tgctgtctgc gcgacctgcg
     3961 gtcgtcgtcg atgcggcaaa cgtcgtgggt tcggtgcccg acggctggtg gaaggatcgc
     4021 gtcggtgcgg cagagcggct gcgtgagcgg ctgtcgacgt ggaccggtgg tggcgtcacc
     4081 gcgcccggtc tcggtgtggg agcggatctc tggtatccgc cggtgtcgat ggtcgtggag
     4141 ggcgaggcgc gggagatcgc ggatgccgtt gatgagcgcg tcgagatcgt gcgcgcgtcc
     4201 ggtgcaggtg acgacgcgat cgtctcggag acgcgacggt tgcgtgatgg cgggttctcg
     4261 gtgacggccg tgaccagcga tcgcggtctg cgagcacggc tggaggaggc cggggctcag
     4321 gtgcagtcgt ccggctggct gctggagcgg ttgggctgag ggaaccaggg gcgctcccga
     4381 cgagatcgcg gcggggcgtc gagtcgacgg cgcgttctca gagatcgttg cgcccgcgca
     4441 ggcgatcgat ctcgcggcgc tcccgcttgg tcggtcgccc cgctccgcgg tcgcggaccg
     4501 cgagcatggc caccgggtca cgcggcgggg tgcgatcgtc gtaggcggtg gccgccacgg
     4561 gtgcgccgac gcgcttggtc agggtctgcc gcacgatgag gatccggtcg aagccggaga
     4621 tccgcacccg cacctcgtcg cccggcttca ccgtctgcgc ggccttgatg cgctcaccgt
     4681 tcacgcgcac gtgccccgcg cggacggctg tcgtggcggc cgatcgggtc ttgtacacgc
     4741 ggacggccca tagccagctg tcgatgcgcg ccggtttcac agcatccgtc atcgtgtctc
     4801 tcgtctcgcg agggtgtgaa gcacgcccca cacgatgagt ccctggccga tcgtgtacgt
     4861 gatcatgacg gtcgggctca tccatccggg caggtcctcg ggcaggaaga gccggaaggc
     4921 gaggatcgag tcgctggcga ggaagaacgc cccgccgatc gtgacgatcg gatggcagcg
     4981 cgccgcggtc acggccgtgc cggcgaggac gagcccgtac gccgccacgg cggggaacag
     5041 gccgccggtg tgaggtccga ggatcgacag catccccacc caccaggcga tgtagacgag
     5101 cgtccaccag ggcaggcgac ggatgcgcgc gaagcggagg aacagcacga tgtagccgat
     5161 gtgcgcgatg ccgaagaagc ccagcatgag gggcagctcg ggcagcgtcg ggaagaaggc
     5221 gccggcggaa tcgcccagcc aggagaagag cagggcggcg agcaggaggc cgacgactgc
     5281 ccgcgggcgc agccgaggcg cggcggcggc gacggggagc gccagcagcg gcatcagcca
     5341 gagcttggtc ggcggcgcca gcgggctgtc cagggcgatc gctgagacat ggatgatcga
     5401 gaggacgatg tacgggaccc aggcgagaac gtcgagaagc cggatgctgc ggggcatccg
     5461 ctcttctccg actgcgtgcg agaccgtggg cgagctcacg actcgaccgt cacggcggcg
     5521 acggtgtcgt cggcggcgag gtgcgcggcg ggccagtagc cctcggggaa gtgctggccg
     5581 caggtgtcca cgaaatgcag cacgatctcg tcgtcggagg atgcctcgat cgcctcgaga
     5641 cgcaggtcgg ctgcgccctc gtccagttcg tgagcctcgg gctcgacgtc gctgaagtgg
     5701 ctgatcacgg catccgacgc gcgccggcgg atgccgtcga tgtcggccac caccgagcgg
     5761 agccggggga gccgcgagcg cacgtcatcg acctcggggc cgtcgaccat cagctcgatg
     5821 tcagcgtcgc cgtcggcgac gctgcccgcg taccagtcat ggacggcgac cgtgccatcg
     5881 tcgaaggtgc tctcggcgcg ggtgaggacg ccgagttcgg ggtcgtcgat cgacgggtgc
     5941 tgtgcgctca tggcttcacg gtagcgcgga cggtcattcc agctcgcgca ccacgaggac
     6001 gcggcctcgg gtgagtggct tcgtctggcg gaatccgtgg gcgaggaacg tggagagcgt
     6061 gccgtggaac aggtcgttgg agtgatgcgt gccctcggag gtgtccaccg gatacgcctc
     6121 gatgagacgc gctccggatt cgcgcgcgta ctcgatcgcg gccttcagga gctgtgcgct
     6181 caggcccttg ccgcgatggt cgcggcggat gacgaagcag gtcaccgccc acacgctcgc
     6241 atcgtccagc ggctcggcgg tggcggcgac gatcgccctg gtgcggacga tgcgcgcctg
     6301 cgcgctgcga ggcccgatgc ggatccagcc ggcggcctca ccgtcgacat aggcgatgag
     6361 tcccggcggg ggaccggccg cgatctcatc tttgagcaag tcgcgccgct gctcgaccgt
     6421 ggtggactgc cactccttgt tgctcacgac gggccagatg cactggcagc tacgcccgtc
     6481 tccgcctccc gtgagggaat gctgcacgtc gtcccaccga ctgatcgtcg cgcgttccgt
     6541 ggtgatcgaa gtcatgcagg gcacgctacg cgcggtatcg gacatcggca acgggccgcg
     6601 cgccacgccc gggcggcgcg gttcgcgcag gccccattcg tcggctagga ttgtggagtt
     6661 gcccgcgctc cggtgcggat cacaacgggc tgtggcgcag cttggtagcg cacttgactg
     6721 ggggtcaagg ggtcgcaggt tcaaatcctg tcagcccgac gtaaacgcac gaggagggtc
     6781 ttgatccgca aggatcgggg ccctcctcgt gcgtttgctc gcacctgtga agaggggcca
     6841 cgcggcgccg agaggctccg cgcgccgctt ctgcgcgcaa cgcgagccga tctcgacacc
     6901 gggcggcgcg tggagggcgc cgtggggatg cagcccgacc gagaagtccc ggaaactcaa
     6961 gggtttccgg gactttttct gcaggtggat gtgatccggt catcgaaaac ccatcacttt
     7021 ggctccctcg cggacctctc aggccgctcc gcgccggcag aggagcgacc gtgcgggttg
     7081 ctcccggtcc gtgggtggga gcaggacgca cctgttcggt atcgacaccg aacaccgccc
     7141 ggcgggcaac ccctggggcc tggatccgct gctcgacgtc ggcgagctcg ccggctatct
     7201 gggcgcgccg tctcgaaggc ctacgactgg cgcactcgag gccgcggtcc gtgggtgtac
     7261 cgctccggga cgtatctgaa gttcaggctc tcggacgtgc ggatttggat cgagcagcaa
     7321 cgcgatcccg aaccgctcgc gccggtcgac cggaggtgac gcgatgagtc ggcagcgttt
     7381 ggccgtcggc accttcgggg agatcggccc cttgtaagcc tcaacggacg cgttgtcgcg
     7441 cgcgcccgct accgtgactg gggactcttc tcgacccagg tatgaatctc cgcaacatgg
     7501 cggcgcacgg attcaagttc gacttcgccc catccgaggc cgcagttctc ttgcgtcttg
     7561 agggcagcgc atcgaaggct ctggcgtcgc ctctcttgga cgtggcgcta gttcccgcaa
     7621 cggtcgaggc caaacgacga cgatgtcgaa cctagcgatt tcgccggtag acagtcccgc
     7681 tgcgcgctga agctgatatc gccagtaacc ggcatggtct tcgatgcagt cagcctcgac
     7741 tccagcgcgt cacccgcacg gcctttcgca tcgcggcgag cagtttgtct tctgcggcgg
     7801 tgattgcatc gttgtcctga acgcggattg caacgaagta ggactcgaca tggttggtta
     7861 ccgacgtcgg tccgagaata gtcaccgcag ccatcgagtc gagagcgcgg gcagtgaacg
     7921 gcttttcgtc cgtcgtggcg gcatcgagtc tcaaccggag gcggtcgacg cgcccggtgt
     7981 aactcgcgtc gggaacggca ggcctcgcat cgttgagggc gtcgaggtcg tgcttcacac
     8041 tggtgatcgc ggcgaagtgc aaatcaaggc cgcgcatcgc tggcacgaac tccttgtacg
     8101 cgtcgaagcg tcgttcacgt agccacttct tgtgctctcg cttcgactgc cagatcgccc
     8161 cgattgatgc ggcaactgcc gtgagggcag ccccgccgag agcggcgatc agggcgaaag
     8221 cgagtgcggt cggatccatg acgcgagcat atcgaccgtc acaccgactc atgcctcaca
     8281 gaggccggct tggagctggg cgtatcgccg tgcagagatc ggcaccgagc ccgagatccg
     8341 gggcgatgag tgtcgttcgc gcgaatcgga tccgctcgcg ggcattgctc gtgagcggat
     8401 ccactgcgtg tggccctcga ggcgctcagt cccgcactgg ccacctgtag tcgacccaac
     8461 ggtcagtagc gcgtcattgg acgtggtgga ccggcatagc gaaatcttca acgccgaccg
     8521 attggtgagc acgtcgctat cgtaagactg gggcatggtg ctcccaaggg gctaggctcc
     8581 ggacctaccg ccattggaga cgaccgctga ctcgggaaat tgagcttcga agcgaaatga
     8641 gacgtctcga atccgaactc actggcggag aaggcgacgc gccggctctc gaccgcctta
     8701 tggagatcag gtgggagttg tggcacacgg agcacctcgc cacggatctc agtgcggcaa
     8761 ttgttacggc aaggagactg ctcgacgcct cagctgacgc ggcgcccgaa acccgagggg
     8821 tgagactcga gcgtctaggt acactgcttg tgattcgata tgaatgcacg ggcaatcgtg
     8881 cggacctaga tgatgcgatc agcgcgctcg agcgggctgt acaagatcta cagcccacgc
     8941 actcgttcta tcccgagtgc gtgtccaccc tttccatcgc gcttgcgctc cgatttgggc
     9001 ttgagagccg ccgagacgac atcgatcgag ccgtcgagct atccgccctc gccgtggcgc
     9061 tcgcccggca gctagacgat gctcgtttgg atgagcgctg tctgcgcgcg ggagcgactc
     9121 tcgccgaccg gtttgagagt cagggcgacg atcaggacct cgtcaccgcc gtcgagtatt
     9181 tccgcgaggc cctcgcagcg actgaaccac tagggggcac ggtttctggt cgctgcgcag
     9241 acctggcgcg cgcattgctg atcaagtatg ggcggtgggc ccgggtggaa gatctggatg
     9301 aagccgccac actgtatcgc cgcgcgatcg acgagctgcc cgccgggcac tacgagatcg
     9361 cctcccacaa gtctggcctc ggtggggtgc tggtcactag gtacgagcac tacggagact
     9421 cagccgatct agcacctgcg atagaactcc ttaccgaggc atacgagttg acggactccg
     9481 cagacccggc gaagccgatt cggcttggca ggttggccga agcgctcatc cagagccata
     9541 gccactttgg tgcaatagca gatcttgata atgcaattag gttgctcgaa tttggcctgg
     9601 aacatcccag tccaaataca cgtatccacg tctccacaca ggtgaccctc gcaggcgccc
     9661 tccgcaggcg cggtctactc cgcaatgata ttggcgacct cggccgcaca gaactacttc
     9721 tccaggaggt ggccgcgaga tccgagcgcg ccgcatacgg cgatgcatcg ttgacgttca
     9781 atctcgcgtc agtgcgttcc gacatgcatt cgatttccgg agaggcgtca catctcagct
     9841 ctgcaatcgc cggcatgcgc gaagctctcg cggtatcacg cgttgactcg cccttgcact
     9901 cgcacgccaa gaccttcctg gcccagctcc tgctgattga ggcagacgac gcaggcgttg
     9961 cgtccgattc cccggtcttc gccgaatgcc ttgccctgtc gcgagaagct gcggttgatc
    10021 caatcttggt tcccgcgctc gggctgatct ccgccgcgac ctgggcggaa cttgcagatg
    10081 gcactgagag actcgttgcg tacgaccgag cactcgcgat tctcccttat gtctccagcc
    10141 acggattgac tgtcgcggac tctctctctc ggctccgtga tctaccgtcc gtcgcgtcgg
    10201 acgcgtgcgc ggccttcgtt gaagctgggc agttggaacg cgctgtcgag gcgcttgaag
    10261 agggccgagc gatcgccgtg aggcttcgca tgcgtcggcg cactgatgtt gaagagttgc
    10321 gtcgcgtgcg cgccgacctc gccgaggcgt atgaagacgc ccttgaaggt acgaaccacc
    10381 cgacctcggg cctcgacgtc gatccgcgcg gcggatcacg gcgcctggaa gaacttcttg
    10441 cacagattcg tgagattacc gggttcgcac gattcggatt tccacgaacc ttccgagaga
    10501 tccaggcact catcggtgac gccatggttg tatacctcaa cacctcttcg agaagatgtg
    10561 acgcgcttgt cgtcactgcg accggggtga ccgtcgtatc gctctcgacg tcggctgcgg
    10621 agatcgcggg tcgggcgact gacttcttga gcgccatgga agagtccgtc gtagcgcggg
    10681 ccgtctccga agtcgtccgt tccgaacgcg tcggaaggga cgtggctgcg tggctgtggg
    10741 atgccgtcgt ccatccggtt ctcgatgagt tgcaactcga cccgcaggag gaaacaaccg
    10801 acgcaaccct gtggcctcga gtctggtgga taccaacggg acacttggct gtgctgccta
    10861 tccaatcggc agggcaccac gcggaatacg gctcgcaggt gatcacctcc tcccacaccg
    10921 cgatcgaccg cgtcgtatca agccagatcc cgtcaatcga gacgctcgcg gcagcgcgac
    10981 aagtttctga cgacggcgct gatgacatcc ggtgtttgat cgttgcttcc tcgcatcctg
    11041 caggcgagag cattctccgc ggagtcgacg aggaggtgga acgtcttgtt gcgcagttgg
    11101 ggcgagagcg gaccacgctc ctcgccgatc aggcgtcgct tctgcctgac ggcccttcga
    11161 caaaggtcgc tgtgttgtcc atgcttgcgc agaataactg ggcgcacttt gcctgccacg
    11221 catcgccttc gcccgagaat ccgacggaga gtcaactgat cctgactgac catcgggcgg
    11281 acccattgcg ggtcgaagat ctgctgacgg ttcgtccgat cgcaccaaga ttggcctact
    11341 tctccgcctg ctcgaccgcg cagatttctc ccgatttggc gcacgagccc gtccatttcg
    11401 gcatcgcggc ggcgctagca ggctatgccc actctgtgct cacgctttgg ccggttcgcg
    11461 acgatccaga gattgcggat gccgtgtaca agggagtgct tcgcgcgatg agctggccga
    11521 tggacgcatc gaccggggcg gccgtccatg atgcgaatcg catgatgagg aagcgaagcg
    11581 cgaacgcgat ccaccgctgg gcggcgacga ttcatcaagg gtcctagaaa tcgaccggga
    11641 catatcgtcg gcttgtcgca cggtggtgct acttcagcgc cctcactcaa cgttgaaagc
    11701 ttccatccgg atcccagttc cgacatgcac gtggtacggc ggaacggagc ccgagaggaa
    11761 gtaaccccat cgactgcggc aacgagcgtg gtgtaccagc ttaggggcgc tactgccgaa
    11821 gggctgtcgc cctgcacgcg gtggcgtcgc gaaggtgcgg cagagtggtg ggctgcgttt
    11881 gctccgatcg catcgagttg tgcccagaag ctgcgtatcg aacgaggagg atctcgatcc
    11941 gcaaggtcgg tgccctcctc gtgcatctgc tcgcgtctgc agcgagggca ccgtgggatg
    12001 ccgcggcgca acgaagccct tggcaagatc gaggcatgga gacgaacgcc cccgaggggt
    12061 ggtatgagga tggcagcggg tcgcagctga ggtactggga cggtgccagg tggaccgatg
    12121 agttcgcacc acggctgccc gcccccgcct cgacatcagt cgttccatcg ccgggcagcg
    12181 atcagaagcg ggagcggaca ggtggtcctg ttctcggcat cgtcggaatc gtcgcggcgg
    12241 ttctggggct cttcggcgtc gtgctgccgt ggatcgccgc agaaactgcg gggattctgt
    12301 tgctcatcgc cgcgttcgcg ctctccggca tagctgccgc ccgcgcgaag cggcgctgga
    12361 tcggggccat cggactgggc atcgcggccg tcgggacggc cgcaggcttc gtgatgttcg
    12421 ccatcggtct ggcacagtca ctcggagcct tccgctgatc gcgatccaga gcaggcgaca
    12481 cagcaccgcc gatcctccga gcgcgccttc cgcctccgtt atggtgggga gcatggagcc
    12541 gaatggcaca gaagccccgg tcggcgcacc tggcaagtcc ggcaagaacg tcgtgaatcg
    12601 ccgtccgcgg gtgggggaga tcgccgacac gctgcgtgag cggatcatct cgggggacct
    12661 gcagccgggg gagcggctgg tggagcagac gcttgcgaac gatctgcggg tgtcgcggat
    12721 gccgatccgc gaggcgctca acatcctccg cggccaggga ttcgtctcgg ccataccgaa
    12781 ccgcggaatg gtcgtgcggc cgctctccga tcaggacatc gagaacctgt tcgaggtccg
    12841 cgaggtgctc gaccagctga cggcacgtcg agcggccgag cgcgcgacgg actgggagat
    12901 cgagcagatg cgcctggcgc tcgagggcgg caccacctcc ctcggtgcgg atgcgacgga
    12961 tgcgcacggc cggatcaacc agggctttca tgacgtgctc accgcggcgg cgcacaacga
    13021 tctgctcgtg acgatcaatg agcccatcga ggggcggctg cagtggctgg tgagccggtt
    13081 cgaccaccct gcgacgtacc accaggagca cctcgcgatc ttcgacgcga tccggcgtcg
    13141 agaccccgat gcggcggcgg cggccgcact cgcgcacctc cagacgagcc gtgaactgtg
    13201 gcgctcctcg caggcgcgcg accgggcgca gatcgcccgc acccgctgac gcgccgagcc
    13261 cgctgacaca acagcaacca accgaccaac cggagcgccc gccggagcga atcagctcat
    13321 ttcgacaaag tgtatgcaat ctctcaaatt cgtatacact ttcacgagag cgatccgtca
    13381 gccgcagatc gtcccgtgtc ggcaaggagg ccagcgtggt ccaacagaac tcttcctcac
    13441 tcatcgagcg catggacagg ctgcccgtca accgggcgca ctggaagatc gccatgatcg
    13501 gcggcctcgg cctgctcttc gacggcatgg acggctcgct ggtctcgtac atcctcccga
    13561 tcctgcgcga caagtgggcg ctcgacggac tgcagtcggg cctgatcggc agctcgctgc
    13621 tcatcggaat gctgatcggc gcgctcaccg ccggcccgat cagcgaccgg atcggccgcc
    13681 ggcgggtgat gatgtgggcg ctgctgctgt tcaccctcgc caccttcgtc gcggcgttct
    13741 cgatgaactg gcagatgttc ttcctgctgc gcgtcctcgc gggcatcggc atcggcgcgg
    13801 aatcggcggt catccccacc tatgtcgccg aactcatccc cgccaagcgc aggggcctct
    13861 tcgccggcgc cgtcgcaggc ttcctcgcct tcggctacgt tctcgcatcg ctgatcgggt
    13921 acttcgtcgt cgcgcggttc gacgacggct ggcgctatgg gcagatcatc accgccattc
    13981 cggtgctgct ggtgctgtgg tggcgccgcg ccatgcccga atcgccccgc tggctgctct
    14041 cccgaggcga gaacgacaag gccgaggaga tcgtcggcgg catcgaacgg ggtgtgctcg
    14101 tccccgtcac ctcgccgctc tcctggaagg cgcagggtga cgggacgaac agccccgcga
    14161 agctgattca gcatcacgag ctgctggggc gcgatctgct cggccgcacg ctgctgctgt
    14221 ggttgatctg gatggcgctc acgttctcct tcttcgggtt cttcagctgg atcccgtcgc
    14281 tcctggtcga ccgcggtctg accgtgacga cgagcttcag ttacgtgctg ctcatgacca
    14341 tcgcgcagat ccccggctac tacggcgccg cctggctcaa cgagaagatc gggcgcaaga
    14401 cgaacatcgc cctgttcctc gcgatcggca cggctggagc atggctgctg tcgcagtcgg
    14461 tcgacccctg gcaggtcatc gcctccggcg ccctgctctc ggcgggtatg aacggcacct
    14521 acgccgccct gtacgcctac acccccgagg tctacccgac ccgcctgcgg gccaccggca
    14581 tgggcatggc ctcggccgtc ggtcgcatcg gcggcatcgc cgctccgatc atcatcggcg
    14641 tgtcccagca ggctctcggc ttcgcgggcg tcttcatcat gatcatgagc gtgctcgccg
    14701 tcaccgcggt gctcgtcgtg gtcttcggcg tctcgacgaa gggacgctcc ctcgaatccc
    14761 tcaccgaagc cgtgcccatc gtggcccaga aggagaactg accaggatgc ccatccgttc
    14821 cgacagccca cgtcccgccg ctccgatcgc gctggtcggc gcccgggtgc tcgacaccga
    14881 cgctaacgtg ctcagcggcc ccgccgtcgt cgtgctgcgg gacgggctca tcgacgaggt
    14941 gcgtgagggc gcggcctcgg cgcccgcggg cacggatgcc gtcgacgtga cgggcagggt
    15001 gctcatgccc ggcctgatcg acgctcacgt gcatgtcacc caggccggca cgcacgccga
    15061 gatccatgac tgggactcca gctatcacgt ggcgcgaagc gcccgggaga tggagcacat
    15121 gctgcggcgc ggattcacga ccgtgcgcga catgggcggc gcggagaccg ggctggctcg
    15181 ggcggtggag gagcgcctgt tcaccgggcc gcggctgctc gcgggcggtc cgatcttctc
    15241 accgaccggt gggcacgggc tcacgcacat cgtcgacggg gaggtcgagc tgcggaaggc
    15301 gctccgcgcg cagttccgcg acggggccga ccacatcaag ctcaccgtct cgggcggcgt
    15361 catcgccaag atgcggatcg aggcgctcgg cttctcggaa gaggagctgc gagcggcggt
    15421 cgacgaggcc cggctggcga aacgctacat cggggcgcac gcctacacgg ctgaagccgt
    15481 gaaccgggcg ctccgcctcg gcgtgcgcac catcgagcac ggcacctacc tcgatgacga
    15541 gtccatccgg ctgctcaccg aactcgagga cgtgcacctc gtgcccactc tggtggcgct
    15601 gtggggcatg ctgcagggcg agtcgcgcgg cacgatcggg gacgatgccc ggcagtcggt
    15661 gcaggatctg ttcgaggtcg gcctgcagac gctgaagacc gccgacagcg ccggtgtgtc
    15721 gatcgggttc ggcacggatc tgcacggcag cggccagcgc ctgcagctcg aggagatcag
    15781 gatcagagcg cgcgtgcaga ctccggaggc gatcctgcga tcgctcacga ccgtcggcgc
    15841 cgacatcgtg cgacgtgcag accggatcgg ccgggtcgca cccggatacg acgccgatct
    15901 gctgctcgtc gacaccgatc cccgtgtgga cctggacgcg gtcgcggatg ccaccaggtc
    15961 gcgcctggtg atcaccgcgg gggagatcgt cgactgaggc gggagctcag tcccagagcg
    16021 ccccgccccg cgcccgtgcc acgacgatcc cgcgctgcga tgtcgggaac cggtccagca
    16081 tggcgcgcag ctccgccgca tcgacgtcgg cgccgaacgc ctcggctccc ggctccagca
    16141 tgatcccgga tgccaggggg ccggcgatca cgggatcgaa tcccacggca tccacgagct
    16201 gggagacgag cgcgagatcg tccgccgcat cgccgcagat ggcgatggca cgccggtcgg
    16261 gatcgccagc ggggcgcgcg tcgtcctcga ggtcctgata gcccatgtgc ccgagcgcct
    16321 tcaccacgcg ggctcccgat aggtgcgcct gcacgagctc gctggtcgac gtgcgcgggt
    16381 cggtcaggtc ggggcggatg ccgtcggcgc cccaccagta gttcatggcg tcgatcacga
    16441 gcatcccgcg cagcaggtcg gcaggcagcg tgcggtatgc gccgagcggg agcgccagca
    16501 ccacgccatc cgcttcggtc gccatccgct cgaccgtcac cgcctgcgcg cccagagcgg
    16561 tcatcgcccg cgagatcggc gccggatcac cggaacgcgc caccagcacg ccgtatccgg
    16621 cggatgccgc gagcgccgcc agcaccgaac ccagccgccc cgcgccgatg atgccgagcg
    16681 tgcggatgcg atccatcccg ccatcgtaag tccgggcatg ccggtggagg tcacggagcc
    16741 cgccgcgcgc cccgctaccg tggaaccggc gctcccgatg cgccggccga tcgacgccga
    16801 tcgaccgcat ccgacattcg cgaaatcagg tcatgtcctc tcgacgcacc cgcgtgctct
    16861 ccgcctgggc ggtgcacgcc ttcaccctca caggggtcct ctgggcgacc ctcgccctgc
    16921 tggccctgtt cgacgacgat cccaagctca tgtggctgta cctgggcatc gccctcgtcg
    16981 tcgacggcgt ggacggcacg ctggcccgca aggcggaggt gcgcacctac gcgccgcact
    17041 tcgacggggt gatcctcgac tcgatcatcg actacctgac ctggacgctg atccctgcgc
    17101 tgttcctgtt ccgcaccgga ctgctcggcg agggcgcgct cgccgtcgtg ctcctgctgc
    17161 tcatcaccgt gtcgtcgatg ttctgctacg cgaacacgaa gatgaagacc ggcgactact
    17221 acttcctcgg cttccccgcg gcgtggaacg tggtcgcgct cgcgatctgg ctgctggacg
    17281 ccggaatcgt ggtcaatgcg atcatcgtgg tcgtgctggc gctgctgacc tgggcgccga
    17341 tcgcgttcgt gcatccgctg cgggtcgcgc ggttccggac gctgaacatc atcgcgaccg
    17401 cggtctggat cctggcatcc gctgccctcg tgatcgccta cccgaccgcc gaaccctgga
    17461 tcgccgtcgt ctggctggtc tcgggcgcct gggtgatcct gaccgggatc atccgcaccg
    17521 tcgcggcgac ccgcgcgctg cggtagcgcc ggcgcagcat ccgctcctgc aggtacttga
    17581 ccacctcgat cgagatgctc gcgatcgtga gcgcggcgat gacggccacc acgaacggcg
    17641 ggtagtcgcg tccgtgcatc cagaagtaga cgtacggcgc cgcgaaggtc gtgatggtgg
    17701 tgagtgtcgc cagcatccgg atccaccggc gcggggcgaa cagcatcgcc agcacgcccc
    17761 aggtgtacgg cacggcggtg aggatgtcga tcatccagag cacgagcagg ctgccatgga
    17821 actgcgggat gaacgccacc ggcagcgccc gcagcgcgga gtagatgccg acggccaggt
    17881 acaccggcac gctcgggtcg cccatcagac ggcggagcgg atgcatgcgc cggacgggcg
    17941 cgtccggggg cagcaggcgc atcgccgccc gcggcacgtg cctggtcggc atcccggtga
    18001 gcgcctcggc ggtctcgacg ggagcggatg ccaggcagaa ccgcagcagt tgcaccacgt
    18061 tcacggcgtg cagtgcgtct tcctcctgcg gcgcgtcatc gacgggatgg acgagccccg
    18121 gaagatcatc cgcgagcagg accccctcca ggggcgacgc gacctccacg aagagcatcc
    18181 ggatgccgtg ctcgagcgcc tcgcgcagca cgacgacgcg ctggacgggg tcgcgcagct
    18241 gggcgggctc gacg
//
LOCUS       DATMSX010000237         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_107941,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000237 DATMSX010000000
VERSION     DATMSX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107941"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VN041_07220"
     CDS             complement(<1..675)
                     /locus_tag="VN041_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HWU28868.1"
                     /translation="MPAAIAVGAVCLAGLAGIPATAAYGADGLAGSIHYASDIAWVSA
                     ENGWGPVELDYNNGARAVPDPDRTIQSIRGTKYPKGLGTHAPAKIVYDVGAQCTQLLG
                     YVGIDDSQNGKGRVDFVVAVDGTEKFRMERTGTSPAGRLNLDLRGASRVELRAEPGPE
                     GNGNDHADWADLKFNCTDGFIAQPLALTAKDVDLSLLAPGMPVSIAIEGAAPRVPVTV
                     AFGDTSV"
     gene            860..2620
                     /locus_tag="VN041_07225"
     CDS             860..2620
                     /locus_tag="VN041_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWU28869.1"
                     /translation="MTKINHSLQESLLLNSTTHPSTEATRSAREVFTAISGLIVGMFV
                     AVLSGTVVSTSLPVIVADLGGTQAQYTWVITASLLATAVTTPIWGKLADLVDRKILVQ
                     LSLVLFTAGTVAAGFSHDINMLIAMRVLQGLGVGGLMSLVMIAVALIISPRERGKYMG
                     VVGGIMALATIGGPLLGGFITDVFGWRANFFVGVPFAIVALILLQITLHLPKPQTAKV
                     SIDYLGIVLLAVGVSALLIWVSMGGDQFEWDSVTSYVLAIGSGVMLLAFVLVEVFVKE
                     PLIPMSLFRNRTFTLSVIASIAIGVSMFATSVFLAQYFQLARGATPTESGLMTIPMIV
                     GQMGASIVIGQLVSRFGKWKSWMLLGSVLALVGVSLMATLRYDTPFALVAVYMFVLGA
                     GLGMVMQNLTLIVQNDTPPQQLGAASSGVNFFRTIAGTIGVTIMGSILATNVKDYIVD
                     GFKGFKPSSPSDLQALKALDSGNVPKMSDLPESIRNIVESAYGHGIADAFVIAIPLAV
                     IGVIAIAFLRNKPLSTKNSAEQLRANAEESAIEVAEAEVGAATGTVRVIGADGAETAS
                     GRVLVLERDDDGADDEDAVR"
     gene            2622..3059
                     /locus_tag="VN041_07230"
     CDS             2622..3059
                     /locus_tag="VN041_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU28870.1"
                     /translation="MATQTADDTLADFQAHMNLIFARAKALWKESAARMHPDLQPSGY
                     KLLAYIARNGDTSAHRLADLFEMDKSVVSRQIRMLEEFGLLQSRPDERDGRLRVLTAT
                     PEASAVLAQLRAENAERMRSVLAELTPEEVQAASKVFRLLAEV"
     gene            3135..>3473
                     /locus_tag="VN041_07235"
     CDS             3135..>3473
                     /locus_tag="VN041_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28871.1"
                     /translation="MSEHASEPTLDGAELDAAVERVERELGRLFARIRIGWREAAATV
                     HPDLQPLGYQVLVGIVSGEATTAGQIIERVQADKSAVSRQVRHLTELGLVDSIRDPDD
                     RRARSLAPTEL"
BASE COUNT          507 a         1210 c         1174 g          582 t
ORIGIN      
        1 gacgctcgtg tcaccgaagg cgaccgtgac ggggacccgc ggcgcggcgc cctcgatcgc
       61 gatcgacacc ggcatccccg gcgccagcag cgacaggtcg acgtccttcg cggtgagcgc
      121 gagcggctgg gcgatgaagc cgtcggtgca gttgaacttc agatccgccc agtcggcgtg
      181 gtcgttgccg ttgccctccg ggccgggttc ggctcgcagc tcgacgcggg atgcaccgcg
      241 gaggtccagg ttgagccggc ccgccggcga ggttccggtc cgctccatgc ggaacttctc
      301 cgtgccgtcg acggcgacga cgaagtccac cctgcccttg ccgttctggc tgtcgtcgat
      361 gccgacgtag ccgagcagct gcgtgcactg cgcgcccacg tcgtagacga tcttcgccgg
      421 ggcgtgcgtg cccagcccct tggggtactt ggtgccgcgg atcgactgga tcgtgcggtc
      481 ggggtcgggc acggcgcggg cgccgttgtt gtagtcgagc tcgaccggtc cccagccgtt
      541 ctcggcggag acccaggcga tgtcgctggc gtagtggatg gatcccgcca gtccgtccgc
      601 accgtaggcg gcggtggcgg ggatgccggc caggccggcc aggcagacgg cgccgacggc
      661 gatggccgcg ggcatccgtg ttcgtctgat gcgcaaggga ttcctcctcg aatcacgcac
      721 ccgggccggg gtggcctcgg atgcccacat cgtgacagca gcggcgcatc aggtgtgttc
      781 aggtgaacac acccgaacac gggggacgga atagttgacc cgaggcaatg gttgagaaag
      841 atcaaccaat gatgtatcgt tgacgaagat caaccattct ctgcaggaga gccttctttt
      901 gaactccacc acacatccct ccaccgaagc cacgcgctcg gcccgagagg tcttcaccgc
      961 gatctccggc ctcatcgtcg gcatgttcgt cgccgtgctg tccggcacgg tcgtgtcgac
     1021 ctcgctcccc gtcatcgtcg ccgacctcgg cggcacccag gcccagtaca cctgggtcat
     1081 caccgcgagc ctgctggcca ccgccgtcac caccccgatc tggggcaagc tggccgacct
     1141 cgtcgaccgc aagatcctcg tgcagctgtc gctcgtgctg ttcaccgcgg gaaccgtcgc
     1201 cgccggcttc tcgcacgaca tcaacatgct catcgccatg cgcgtgctgc agggcctcgg
     1261 cgtcggcggc ctgatgtcgc tcgtcatgat cgcggtcgcg ctgatcatct cgccgcgcga
     1321 gcgcggcaag tacatgggcg tcgtcggcgg catcatggct ctcgccacga tcggcggccc
     1381 gctgctgggc ggcttcatca ccgacgtgtt cggctggcgc gccaacttct tcgtcggcgt
     1441 gcccttcgcg atcgtcgcgc tgatcctgct gcagatcacc ctgcacctgc ccaagccgca
     1501 gaccgcgaag gtctccatcg actacctcgg catcgtgctc ctggcggtcg gcgtctcggc
     1561 gctgctgatc tgggtgtcga tgggcggcga ccagttcgag tgggactcgg tcaccagcta
     1621 cgtgctcgcc atcggctccg gcgtgatgct gctcgcgttc gtgctggtcg aggtgttcgt
     1681 caaggagccc ctcatcccga tgtcgctgtt ccgcaaccgc accttcacgc tctcggtcat
     1741 cgcctcgatc gccatcggcg tgtcgatgtt cgcgacctcg gtgttcctcg cgcagtactt
     1801 ccagctcgcc cgtggcgcca caccgaccga gtcgggcctg atgacgatcc ccatgatcgt
     1861 cggccagatg ggtgcgtcga tcgtgatcgg ccagctggtc agccgcttcg gcaagtggaa
     1921 gagctggatg ctgctgggct cggtgctcgc gctcgtcggc gtcagcctga tggccacgct
     1981 gcgctacgac accccgttcg ccctcgtcgc ggtctacatg ttcgtgctcg gcgcgggcct
     2041 cggcatggtg atgcagaacc tcaccctgat cgtgcagaac gacaccccgc cgcagcagct
     2101 gggcgcggcg tcgtccggtg tgaacttctt ccgcacgatc gccggcacca tcggcgtgac
     2161 gatcatgggc tcgatcctgg ccaccaacgt caaggactac atcgtcgacg gcttcaaggg
     2221 cttcaagccc tcgtcgccgt ccgacctcca ggcgctgaag gcgctggact cgggcaatgt
     2281 cccgaagatg tccgacctgc cggagtcgat ccgcaacatc gtcgagtcgg cgtacggcca
     2341 cggcatcgcg gatgccttcg tcatcgccat cccgctcgcc gtgatcggcg tcatcgccat
     2401 cgccttcctc cgcaacaagc cgctgtcgac gaagaactcc gccgagcagc tgcgcgcgaa
     2461 cgccgaggag tcggcgatcg aggtcgccga ggccgaggtg ggtgccgcca ccggcaccgt
     2521 ccgcgtcatc ggcgcggacg gtgcggagac cgcctccggc cgcgttctgg tgctggagcg
     2581 cgacgacgac ggcgcggatg acgaggacgc ggtgcgctga gatggcgacg cagaccgccg
     2641 acgacacgct cgcggacttc caggctcaca tgaacctgat cttcgcgcgt gcgaaggcgc
     2701 tgtggaagga gtcggccgcc cggatgcacc cggacctgca gccctccggc tacaagctgc
     2761 tggcgtacat cgcccgcaac ggtgacacga gcgcgcaccg gctcgccgat ctgttcgaga
     2821 tggacaagtc ggtcgtgagc aggcagatcc ggatgctcga ggagttcggc ctgctgcagt
     2881 cgaggccgga tgagcgcgac ggccgcctgc gggtgctgac ggcgacgccg gaggcctcag
     2941 ccgtgctcgc ccagctgcgt gccgagaacg ccgagcggat gcgctcggtg ctggccgagc
     3001 tgacgccgga agaggtgcag gccgcctcga aggtgttccg gctgctcgcc gaggtctgac
     3061 ccgcggcatc cgctccaccg tcctgaagcc cgcctcccga ttcgggaggc gggcttcgcc
     3121 gtaagctggc gggcatgagt gagcacgcgt ccgaacccac gctcgacggg gcggagctgg
     3181 acgccgccgt cgagcgcgtc gagcgggagc tcggccggtt gttcgcccgc atccggatcg
     3241 gctggcgcga ggcggccgcg accgtgcacc ccgacctgca gccgctgggc taccaggtgc
     3301 tggtcggcat cgtcagcggc gaggcgacca cggccgggca gatcatcgag cgcgtgcagg
     3361 cggacaagtc cgcggtcagc cggcaggtgc gtcacctcac cgagctcggt ctggtggaca
     3421 gcatccgcga ccccgatgac cgccgcgcgc gcagcctggc gccgacggag ctg
//
LOCUS       DATMSX010000238         8324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_164354,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000238 DATMSX010000000
VERSION     DATMSX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164354"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VN041_07240"
     CDS             <1..336
                     /locus_tag="VN041_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpcA/G6PD domain-containing protein"
                     /protein_id="HWU28872.1"
                     /translation="AASPSTALLAAWLRMALDVPVSWSYEDPEHWQEGIKSVRLTRAS
                     GDILLERPVPGTAVLTQPGQPDHELHLPRRTLRECLAEELRRLDADVLYGRVITEGWE
                     RLGPADKGA"
     gene            342..1127
                     /gene="pgl"
                     /locus_tag="VN041_07245"
     CDS             342..1127
                     /gene="pgl"
                     /locus_tag="VN041_07245"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWU28873.1"
                     /translation="MVTTVQGSVKSVVVEPTPASLAARVADRFLTRVVARTRNGRIAH
                     ICLTGGSMGGAVLAAAADDERVATIDWSLVHFWWGDERFVERDDPDRNSLQSRRALLD
                     RLDIPAENIHEVAAPSDGLSLDEAAAAYAAELARFGRSSEAWPSFAVCFLGVGPDGHI
                     ASLFPDRQEVTVTDAAVLPVRNSPKPPPERVTLTRPVLNHSKRVWLVLTGADKASALG
                     LALAGASYASVPAAGAKGRRRTVFFVDEAAAAEVAPELIDRDY"
     gene            complement(1121..2143)
                     /locus_tag="VN041_07250"
     CDS             complement(1121..2143)
                     /locus_tag="VN041_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU28874.1"
                     /translation="MTGVSDQLIGVFQNPALLLGIPLALAGAVFMSLGAQYQHRGVEK
                     VEKLTGTEGASGLTGAQIKGLLTRPSWIIGTLMLGFAVICQLSAMAIAPLIVVQPLGA
                     VALVVTTLLNARVSGHTPTKKSLIAIGSCLGGIFLFVGLAAFYAVERKISDEDLFTIL
                     AILLVVIIVLGTFWLILRHRMRALFYVIGAGILYGFVATLAKAVISRIQNGNFEWLTV
                     ACLIALFAAAAVGAYFVQSAYSSGPPDLVIAGLTVVDPIVAVLIGALVLGEASEAPLP
                     VLILFGIAGVIAVFGVFGLARYHPQVLSDSQELAIQRGSDASTGSAAQDQSRGPREQA
                     ESPDAQ"
     gene            2227..2793
                     /gene="def"
                     /locus_tag="VN041_07255"
     CDS             2227..2793
                     /gene="def"
                     /locus_tag="VN041_07255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWU28875.1"
                     /translation="MAVLPIRIMGDPVLHAPAAEVSEITDEIRTLVADMYETMDVAPG
                     VGLAAPQVGVALRIYVYTYVDDEDRPWRGEIINPVLWMTPPEPGSPDPDEESEGCLSF
                     PGERFPLRRSDRVMVTGIDLEGAPVRIEVDGWRARIMQHEFDHLNGILYIDRLSDGDW
                     KTVQKIARKRGWARGGASWMPGVDDLEG"
     gene            2880..3398
                     /locus_tag="VN041_07260"
     CDS             2880..3398
                     /locus_tag="VN041_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HWU28876.1"
                     /translation="MTSQRALLRGTTLTGAAIILTALLSGCSVPTNLFSGDAQRDDKG
                     NVTDGSSIDIFKLKVGDCKFEDDQSSTELSDTNVVPCDEAHDEEVYAEFTLPDGKFPS
                     EDALDAAADPKCGEEFTKFVGLAVDASKLDYFYYSPTKQGWDELDDRLIQCVLYADDE
                     SQLTGSMKGSGA"
     gene            4170..4391
                     /locus_tag="VN041_07265"
     CDS             4170..4391
                     /locus_tag="VN041_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28877.1"
                     /translation="MAMISAPTIGPIGGALIAITVLAASIPAQSTTCRALLAVMRCGD
                     LDEDTEARTIDVSKAAMIAWFDMPMNQDP"
     gene            4724..5614
                     /locus_tag="VN041_07270"
     CDS             4724..5614
                     /locus_tag="VN041_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HWU28878.1"
                     /translation="MTEPLKLRASRRYGTLSPLRYPGGKASLAGFFADLIAELGLAGA
                     TYVEPYAGGVGAGIALLRENLVERLVINDFDPAVYAFWASVTTRPSDFIDLVVRTPLD
                     IEEWQRQRAIYREARTDDLLELGFAFFYLNRTNRSGVLTGGVIGGLAQTGNYKMDARF
                     NRGTLIDRITAIAELSERILVTSRDGRSVIREYASSSDVFMYIDPPYVQAGSRLYMNA
                     FEGRDHAALADVVDGIDSAHWLMTYDVDPLIERLYSQHFQTRYELYYSARNYIMSDEL
                     LIASPRVADAITQKVLKVPA"
     gene            complement(5583..7085)
                     /locus_tag="VN041_07275"
     CDS             complement(5583..7085)
                     /locus_tag="VN041_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28879.1"
                     /translation="MPYTPFPIVDVDVTALHLDRSNFRIDDVLPSESAAITYLFAEHD
                     VLGLARQILIEGYVDNELILAIEEDGKYVVLEGNRRTTALKALLDPSVAPGEARASLE
                     LLKRRHEIEVDDLPAKVRVMVAPNRAATQMLLARLHIGQSKIGWGRDAQARFVIAQLE
                     SGMSISMIRQELPGVKNAAAYVRQYHVRKILKEARFKDPSVAKYAASPRLRMTSFEYA
                     YGNPDIQKVLGLSFDKEGVPTSIPTSSVEIQALERLVALFQSNELNTRKFPKRTSVTY
                     DTEMRTLIAALTGKEPEKSGASTKSDSPDDESQVPEGESGDGGDPEGSGESTAKGGSV
                     GGGAPSGGEAGGGRGRGPNNPDTLKTLHIALDYRDAPVGVRKRLNELRSLDLGNHPIA
                     AAVLMRSVIEASIKWHFSARSAHVSGELGTVMGEVAKSYASHRPLRNAIELLRDGSRR
                     NVRSGSLHWFNMAAHDSNHVVDAQSVRDAWQHVEQFVGFMLTPAPSTPSA"
     gene            7739..>8324
                     /locus_tag="VN041_07280"
     CDS             7739..>8324
                     /locus_tag="VN041_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28880.1"
                     /translation="MSPVDIHAPERERFIDSHAGAQHHIHNATKEERRRGDVSLGSNR
                     PGGHAPPNNLDLLRGQRRGGGFHSALPAQQANRIAVAKPMGECHSECTAERRSREGVG
                     AASALKLDLLKNPSYPRGALRILQPHCTDERNQPSRRVPINSQRLRSHFAGSIFEPEV
                     KRSSQREISCSAAVHESETVHTSFKPPRPRKTRSI"
BASE COUNT         1524 a         2760 c         2612 g         1428 t
ORIGIN      
        1 gcggcctcgc cgtcgacggc cctgctggcc gcctggctgc ggatggcgct ggacgttccg
       61 gtgagctggt cctacgagga cccggagcac tggcaggagg gcatcaagtc ggtgcgcctc
      121 acccgcgcca gcggcgacat cctgctcgag cgccccgtgc ccggcacggc cgtgctcacc
      181 cagcccgggc agcccgacca cgagctgcac ctcccgcggc gcacgctgcg tgagtgcctc
      241 gcggaggaac ttcgccgcct cgacgccgat gtgctgtacg gtcgggtcat cacggagggc
      301 tgggagcggc tgggccccgc agacaaggga gcgtgagttc gatggtgacc acggtgcagg
      361 gatcggtcaa gagcgtcgtc gtggagccga cccccgcgag tctggccgcg cgcgtcgcgg
      421 atcggttcct gaccagggtc gtcgcacgca cccgcaacgg ccgcatcgcg cacatctgcc
      481 tgaccggagg atcgatgggc ggcgcggtgc tcgccgctgc cgccgacgat gaacgggtcg
      541 ccacgatcga ctggtcgctg gtgcacttct ggtggggcga cgagcgcttc gtggagcgcg
      601 acgaccccga ccgcaactcc ctgcagtcgc gccgcgcgct gctggatcgt ctcgacatcc
      661 ccgcggagaa catccacgag gtggccgcac cctcggacgg gctgagcctg gacgaggcgg
      721 cggcggccta cgcggccgag ctggcgcgct tcggccggtc gagcgaggca tggccatcgt
      781 tcgcagtgtg cttcctgggc gtcggtcccg acggccacat cgcctcgctc ttcccggacc
      841 gtcaggaggt gacggtgacg gatgccgcgg tgcttccggt gcgcaactcc cccaagccac
      901 cgcccgagcg cgtcacgctc acccgcccgg tgctcaacca ctccaagcgg gtctggctcg
      961 tgctgaccgg cgccgacaag gcatccgccc tcggcctggc tctggcaggt gccagctacg
     1021 cgagcgtgcc ggccgcggga gccaagggac ggcgccgcac ggtgttcttc gtcgacgagg
     1081 ccgccgcggc cgaggtcgca ccggagctca tcgaccgcga ctactgagcg tccggcgact
     1141 ccgcctgctc ccgaggccca cggctctgat cctgggccgc tgagcctgtc gaagcgtcgc
     1201 tcccccgctg gatcgcgagc tcctggctgt cgctgagcac ctgcgggtgg taccgggcca
     1261 ggccgaacac gccgaacacg gcgatcacgc cggcgatccc gaacaggatc agcaccggca
     1321 gcggcgcttc ggatgcctcg ccgagcacga gcgcgccgat gaggacggcc acgatggggt
     1381 cgacgacggt gagaccggcg atcacgaggt cgggcgggcc cgagctgtac gccgactgga
     1441 cgaagtacgc gccgacggcg gcggcggcga acagcgcgat gaggcacgcg accgtcagcc
     1501 actcgaagtt gccgttctgg atgcggctga tcacggcctt cgcgagcgtg gcaacgaagc
     1561 cgtacaggat tcccgcgccg atcacgtaga acagcgcgcg catccggtgg cggaggatga
     1621 gccagaacgt gcccagcacg atgatgacca cgagcaggat ggcgaggatc gtgaacaggt
     1681 cctcgtcgct gatcttgcgc tccaccgcgt agaacgcggc caggccgacg aacaggaaga
     1741 tgccgcccag gcaggagccg atcgcgatca gcgacttctt cgtcggggtg tgccccgaca
     1801 cgcgcgcgtt cagcagcgtg gtgaccacca gcgcgacggc gccgagcggc tgcacgacga
     1861 tcaggggcgc gatggccatg gcgctgagct ggcagatcac ggcgaagccc agcatgagcg
     1921 tcccgatgat ccaggacggc ctcgtcagca gccccttgat ctgggcgccg gtgagtccgg
     1981 aggcaccttc ggtgccggtc agcttctcga ccttctcgac cccgcggtgc tggtactgcg
     2041 cgccgagcga catgaacacc gcgccggcca gtgccagcgg gatgcccagc agcagcgcgg
     2101 ggttctggaa cacgccgatg agctgatcgc tcactcctgt cacgactccc accccaagct
     2161 gcactgtttc accctacccg gcccgacggt cccgtccggg ccgaacgcgc ccagtagggt
     2221 ggtggcgtgg ctgttcttcc gatccgcatc atgggcgacc ccgtgctgca cgcccccgct
     2281 gccgaggtct ccgagatcac cgatgagatc cgaacgctcg tcgccgacat gtacgagacc
     2341 atggacgtcg cgcccggtgt gggcctggcc gcaccccagg tgggcgtcgc gctgcgcatc
     2401 tacgtgtaca cctacgtcga cgacgaggac cgaccgtggc gcggggagat catcaacccc
     2461 gtgctctgga tgacgccgcc cgagcccggc tcccctgacc cggacgagga gtcggaggga
     2521 tgcctgtcgt ttcctggcga gcggttcccg ctgcgtcgct ccgaccgcgt gatggtcacc
     2581 ggtatcgacc tcgagggtgc gcccgtgcgc atcgaggtgg acggatggcg tgcgcgcatc
     2641 atgcagcacg agttcgacca tctcaacggc atcctctaca tcgaccgcct ctcggacggc
     2701 gactggaaga ccgtgcagaa gatcgcccgc aagcggggct gggcgcgcgg cggcgcgagc
     2761 tggatgcccg gagtcgacga cctcgagggc tgatacccgg tcgacttgcc gcggaatcgc
     2821 gagggtcagt acggtggggt gcgattcaca gcatccatcc cccgatctag gaccagcaca
     2881 tgacctctca gcgagccctg ctccgcggca ccaccctgac cggtgcggcc atcatcctca
     2941 ccgctctcct cagcggctgc agcgtgccga cgaacctgtt cagcggtgac gcccagcgcg
     3001 acgacaaggg caacgtcacc gacggctcga gcatcgacat cttcaagctg aaggtcggcg
     3061 actgcaagtt cgaggacgac cagagcagca ccgagctcag cgacaccaac gtcgtgccct
     3121 gcgacgaggc gcacgacgag gaggtctacg cggagttcac gctccccgac ggcaagttcc
     3181 cttccgagga cgcactcgac gcggccgccg acccgaagtg cggcgaggag ttcacgaagt
     3241 tcgtcggcct cgccgtcgac gcatccaagc tggactactt ctactactcg cccaccaagc
     3301 agggctggga cgagctggac gaccgcctga tccagtgcgt gctctacgcg gatgacgagt
     3361 cccagctgac cggatcgatg aagggctccg gcgcctgacc cgttccggac cgacgccgct
     3421 gtgatccgcg tccctcacca atcgcgcgga ccagggttcg gattgaaagt cgggcgctgt
     3481 cgcctgccct ctacgtagtc ggtgatatcc cgcgggggcg ggcgacgacc ggatgccgtc
     3541 gtcgttccgg tctggcgata ttcggcgaag agctcgggtt ctcgacgcag cgcagtacgc
     3601 ttccactgtc gtttgattcg cctattgttc cggcgagctc gacgtgctgg cgctccacgt
     3661 acccaggcat aaacgcagat ggtcaacagc accccgacca atattccgac cgcgccaccc
     3721 gcgatgatca gcagtgattc acgagtggcc agaactcccc agtagtccac ggccaaacca
     3781 tagcggacat cagagcaatc ggacagatgt ccggacggtc ccggagacga cgaaaccccg
     3841 ccacgagggc ggggtttcgg tggggttggc tccccggctt ggactcgaac caagaacctg
     3901 ccgttatggc acctcatggt tcgagcacgc caggaggcgt cacatcatga aagataggct
     3961 gcgaaaaccc ccggtcagga aggttaacta tccgctcccg ccatgtatct cgcaggaatc
     4021 aggatgccgg ttgcgacggg atcagccgaa ctgaatcggg ttgaatcgcg tgggtagggc
     4081 gagtttcgcg tgagtaaaag gtgagtgccg gatcgatgcc ccgcggcacc tgaccacgca
     4141 actgccaccc gcctcgagga gcaatgaaag tggcgatgat cagcgccccg acgattggcc
     4201 cgatcggcgg ggcgttgatc gcaatcacag tgctcgcagc cagcatcccc gcgcagagca
     4261 ccacctgtcg ggcactcctt gctgtgatgc ggtgcggtga tctcgacgag gatacggaag
     4321 cccgaacgat cgacgtcagc aaggcggcaa tgatcgcatg gttcgacatg cccatgaacc
     4381 aagacccgta gaacccgcac gcgggtcaag agcacgccta gtgaccaagg cgcctggtga
     4441 ggtcttcgca acttgccgtc tcgatgcttc agttggagat gtgaaacagt tactatctgt
     4501 ttcatgcccg tgatgaactt gccctcgcca gacgaactcg agaacctcga ggccgaacgt
     4561 ctgacgaagt tgggcggcct ggtcaagcat gaacatctgc gccggcgaaa cgcctttcgc
     4621 gtctgcgcac actgtggcgc tagttggcta gcccgagcaa acgcccgata ctgtcagccg
     4681 agctgtcgcc ttgcagcatt ccgcgagcga aagaaggcga cgcatgactg aaccgctgaa
     4741 actgcgcgcg tcgcgtaggt acggaacgct gtcgcccttg cggtacccag gaggaaaggc
     4801 gtcccttgca ggtttcttcg ccgacctcat tgcggagctt ggactggcgg gcgccacata
     4861 cgtcgagcct tacgcaggcg gtgtcggagc tggcatagcg ttgcttcggg agaacctcgt
     4921 cgagcgtctg gtcatcaacg acttcgaccc tgccgtgtac gcgttctggg ctagcgtgac
     4981 gacaaggccg tccgacttca tcgacttggt cgtccgaacc ccgttggata tcgaagaatg
     5041 gcaacgacag cgtgcgatct atcgagaggc acgaaccgat gaccttctgg agctcggttt
     5101 cgcgttcttc tatctcaacc gcacaaaccg ctcgggggtc ctcactggcg gcgttatcgg
     5161 aggtctggcg cagaccggaa actacaagat ggacgcccgg ttcaatcgcg gcaccttgat
     5221 cgacagaatc acggcgatcg cggaactctc tgagcggatc ctcgtcacct cgcgcgatgg
     5281 gaggtccgtc attcgagagt acgcgagcag cagcgatgtc ttcatgtaca tcgatccccc
     5341 ctacgtccag gctgggtcgc gactgtatat gaacgcattc gaggggcggg accatgccgc
     5401 tctcgcagac gtcgttgacg ggatcgacag cgcacactgg ctcatgacct acgacgtcga
     5461 ccctctcatc gagcgtctgt actcacagca ctttcaaact agatatgagc tgtactactc
     5521 ggcccggaac tacatcatgt cggacgagct gctgattgcg tccccccgcg ttgccgatgc
     5581 gatcacgcag aaggtgttga aggtgccggc gtaagcatga agcccacgaa ctgctccacg
     5641 tgctgccacg catctcgaac gctttgagca tcgaccacgt ggttggagtc gtgcgccgcc
     5701 atgttgaacc aatgcaacga ccccgagcgg acgttccttc gggatccatc tcgcaatagc
     5761 tcgatcgcgt tgcgtagcgg acgatgcgag gcgtacgact tagcgacctc ccccatcaca
     5821 gtccccagtt caccactcac gtgggcgctc ctggccgaga agtgccactt gatgctcgcc
     5881 tcgatcacgc tgcgcatcag aacggcggcc gcaatcgggt ggttgcccag gtcgagcgag
     5941 cggagttcgt ttagcctctt gcgcactccg accggggcgt cgcgatagtc gagggcgatg
     6001 tgcaaggtct tcagcgtatc cgggttgttc ggcccacgcc ccctaccgcc tccagcctca
     6061 ccgccagacg gcgccccgcc accaacgctt ccaccctttg cggttgactc gccgctacct
     6121 tcagggtcgc caccatcgcc cgattcgccc tcagggacct gcgattcatc gtcgggcgag
     6181 tcgctcttgg ttgatgcgcc cgacttttcc ggctctttgc ctgtcagcgc cgcgatcagc
     6241 gtacgcatct ccgtgtcata ggtgacggaa gtacgcttcg ggaacttacg agtgttcagc
     6301 tcgttcgact ggaagagtgc cacgagcctc tcgagcgcct gaatctccac agaagatgtg
     6361 gggattgacg tgggcacgcc ttctttgtcg aagctcagtc cgagaacctt ctggatgtcg
     6421 ggattgccat acgcatactc gaacgacgtc atcctgaggc gtgggctcgc tgcgtacttg
     6481 gccacgcttg gatccttgaa tctcgcttcc ttgagaatct tcctgacgtg gtactgacgc
     6541 acgtacgccg ctgcgttctt aaccccaggg agctcttgcc gaatcatcga gatcgacatt
     6601 cctgattcaa gctgggcgat cacgaaccgg gcctgcgcgt ccctccccca cccaatcttc
     6661 gactgcccaa tgtgaagacg cgccagcaac atctgggtcg cggccctgtt cggcgccacc
     6721 ataactcgga ccttcgcggg gagatcatcg acctcaatct cgtgccttcg tttgaggagt
     6781 tccaggctgg ccctggcctc ccccggagca actgaaggat caagcagagc cttcagcgcg
     6841 gtcgtgcgcc gatttccctc aaggacgacg tacttgccat cctcttcgat ggccaggatc
     6901 agttcgttgt cgacgtatcc ctcgatcagg atctgacgag caaggccaag aacatcatgt
     6961 tcggcgaaca gataggtgat cgccgcggac tcgctcggga gcacatcgtc gattcggaag
     7021 ttgctccgat cgaggtggag tgcggttacg tcgacatcaa cgatcggaaa cggcgtgtac
     7081 ggcatcgagc ttccccccgg gagttcgagt agggctagtt cgccctatta tggctcatgc
     7141 cctcggaatg acgaccttgc gcaacgatgc cgcaagtgtg tcgccacctt tgtagaagtg
     7201 gcctactcgg aggcgggccc ccgacactcc gctaacagtc gtcgatacgc ccgtacccga
     7261 gcaggcgttc cagagcgtcc gctgagtcca cccgacgaaa cgcaacgccc acgtagtgtc
     7321 ggatcgtggt gcgaggatcc gtatggccca ggaggcgctg ggctgcaccg atctccccgg
     7381 atgagaccgc cacaagagtt gccgcagtgc gacgaaacac atgtggagtg agatcgctcc
     7441 gaaccaaccc cgtcaacgcg agcgctgcga ctagttcccg gtcctcgcga aggcttcgga
     7501 gagaccgcct gatgttgcgc gcgtgacacc acgctccaac gacccgcccc tgtatcgccg
     7561 ggctcaccgg ggtgagcgcc ccttcgtcgg gcacgcgctg tcgtgcgtca tgaagtgcgc
     7621 gaatcgcgaa ctgaggaaga tgaatgaccc gggcttgact ggaccctttc aggagatcct
     7681 ggcggagcac tcgatggctt gaggtatcca cgagcgtgcc tgtgactgcg atagtggaat
     7741 gtcgccagtc gacatccatg caccggagcg cgagcgcttc attgattcgc acgccggagc
     7801 ccaacatcac atccataacg caaccaagga ggaacgccga agaggcgacg tatcgctcgg
     7861 ttcgaatcgc ccgggcgggc acgcaccgcc gaacaatctc gacctgctcc gggggcagag
     7921 acgtggcgga ggctttcact cggctttgcc ggcgcagcaa gcgaaccgga ttgccgtcgc
     7981 aaagcccatg ggcgagtgcc actccgaatg caccgctgag cgcagatcgc gcgaaggagt
     8041 aggcgccgca tccgcgctta agctcgacct cctcaagaat ccttcgtatc cgcgcggggc
     8101 gttgcgcatc ctccagccgc actgcaccga cgagaggaat caaccatctc gtcgtgtccc
     8161 gataaactcg cagcgtttgc ggtcgcactt cgcgggctcg atcttcgagc cagaggttaa
     8221 gcgctcctcc cagcgtgaga tctcctgctc tgccgccgtc catgagagcg agacggtgca
     8281 cacttcgttc aagccccccc gcccaaggaa gacccgctca atcc
//
LOCUS       DATMSX010000239        12794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_145492,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000239 DATMSX010000000
VERSION     DATMSX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145492"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..845
                     /locus_tag="VN041_07285"
     CDS             177..845
                     /locus_tag="VN041_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HWU28881.1"
                     /translation="MSTVPVDEAAIAEAKQLRDQFQRFLREYEFGMREVETKISILRD
                     EFTHMHAYNPIEHVSSRLKSPDGIVEKVARKGIDPDFDVIRREITDIAGVRVTCSFVT
                     DVYRLFELFTRQDDITVLMVKDYIAEPKPNGYRSLHAIVEVPVFLSTGAIPVPVEVQF
                     RTIAMDFWASLEHKIHYKFDGRVPAHLVQSLTEAADAAGELDLRMERLHREALEINHR
                     VIEA"
     gene            complement(953..2167)
                     /locus_tag="VN041_07290"
     CDS             complement(953..2167)
                     /locus_tag="VN041_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWU28882.1"
                     /translation="MSIDLEALYIDLHQHPELSFQETRTAGIAAGHLRDLGLEVHEGV
                     GGTGVVGVLANGEGPTVWVRADMDALPVTEETGLPYASKATGIDPDGNEAGVMHACGH
                     DMHVTAMIGAIEQLVAEQNQWHGTLVVVIQPAEELGAGARAMLDDGALDRFPKPDIVL
                     GQHVGPLPAGAVGLRPGPQMSASDGLHVVLHGQGGHGSRPDSTIDPVVMAAATIMRLQ
                     TIVSREVDPTGMAVVTVGAVHAGTKNNIIPAEATLDLSLRYPDDAAREKVLKKVERVI
                     RAEAAASGAERDPEISTLHTLPATINDADATARATSAFQHAFGEQAVIDTGLITGSED
                     VSWFTRDAGVPLVFWFWGGVDPAVFAAAVQNDSVDHDIPTNHSPFFAPVIHPTIEVGV
                     KALVTAAREFLG"
     gene            2428..2631
                     /locus_tag="VN041_07295"
     CDS             2428..2631
                     /locus_tag="VN041_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWU28883.1"
                     /translation="MATGTVKWFNSEKGFGFIAPDDGSADLFAHFSAIASSGYKELTE
                     NQKVEFDAERGPKGMQAMNIRPL"
     gene            2761..3297
                     /locus_tag="VN041_07300"
     CDS             2761..3297
                     /locus_tag="VN041_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28884.1"
                     /translation="MTKDETRPAGFGALLWLAMATFSMGIDGYVLAGLLPQIAKDLDV
                     SEPAAGQLVAVFALTAALAGPVLSALTGAWERKGIISASLAVFVVGNLVIALSVNYTM
                     AMGGRILSAIGGSLLSAVVSAYVLAKTAPERRGRALSFVLGGFLAATALGSRSDSSSA
                     RRTGGCRCTWSPGSAPSR"
     gene            3309..3950
                     /locus_tag="VN041_07305"
     CDS             3309..3950
                     /locus_tag="VN041_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28885.1"
                     /translation="MNVPRLHLPALSLRETLKPLARPALVGSILVATGIMCASYLCFT
                     YATLIFGPRVGEGPAIIAAMFGYGVVSLFGNIVSGRATDRFNPVHVISLIVGILIVVA
                     LLGTAGLLLPGAAGAVAAFAWFFCCAFFNGGSGVALQSRLGDLAPDAMSLVLALNGSG
                     MQLGSALGGMLGGALLASGLAADGLVPASAVVLAATMILHLIVSRAKPAARTA"
     gene            complement(3951..4592)
                     /locus_tag="VN041_07310"
     CDS             complement(3951..4592)
                     /locus_tag="VN041_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28886.1"
                     /translation="MPKATQDTPRTRAAQHKRIEILNAAVEIFGSKGSTNGTLADVAE
                     QVGLTHAGVLHHFGSKQRLLLEVLAYRDQADVADLDDKHIPGGLELFLHLVRTAFANE
                     RRPGIVQAYTVLSSESVTDDHPGREYFEDRYITLRREVTEAFEVLCRAEGVTDTDTIA
                     HAAAAILAVMDGLQLQWLLHPEIVGLGESSEFAITAIVNGVLHPGPPLESYRR"
     gene            4759..6432
                     /locus_tag="VN041_07315"
     CDS             4759..6432
                     /locus_tag="VN041_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28887.1"
                     /translation="MKLKKYLIAGTAIAALSALALTGCSSSGSAGSGGGGTSLTVAKP
                     DGAGVLSTQINNPFIGTGSGMSLAYDRMIYEPLAMVNPVGKNETTPWLASKVEWNSDY
                     TQLTVTPRKDVKWNDGKPFTADDIVFTFTLIKNTPALDLGGLKLSGIEKDGDDVVMTF
                     SESKFVKQGDVLQVPIVPEHQWKSIADPTKDPVKDAIGTGPYTIKSFSSQGVVLSARS
                     DYWGGKVPVATLKYLEYNDNTGLLRALQSKEADWAQIFITDYQTNFVDKDPKHNVFWG
                     ANVLSPDMILVNTTKAPFNDVAFRKAVNMVVDRKAHAEKARSNAGPELKNVTGIPQPT
                     GDQYIAPEYKDKNFTVDVDGAKKILTDAGYTWKGGALIDPSGNPVSFTLQDPQGWNDY
                     VTGIQLVATQIKSTLGADAKVATPDADTWFSNMATGNFDAALHWSGSGSNPWHIYDDV
                     MNGAYLEQASGGKVSDNFGRYDNPEATALLKQYAQASDDAARTDALNKIQKIFVEDVP
                     AIPIGTHPQLAEYNTRTFTGWPSESDQYATADPTQPSAVQVLMNLKPVK"
     gene            6499..7323
                     /locus_tag="VN041_07320"
     CDS             6499..7323
                     /locus_tag="VN041_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28888.1"
                     /translation="MTDPLLSIRDFSVVYDVDPPVEAVKHVTLDIRRGEIIGLAGESG
                     SGKTTLAYGVQRLLKPPAVITSGSVVFHDEDGADIDLHALDVEQMRRFRWDKVSMVFQ
                     GAMNALNPVATIGSQLDDVFVVHRPRMGRRERREAVDELLEIVKVGAQRARSYPHELS
                     GGMRQRVMIAMALALRPQLMVMDEPTTALDVLVQREILKQISQLRHEFGFSVVFITHD
                     LPLLLEISDRIAVMREGEIVELSDARELWTNPRHEYTQNLLRSFPRLTGERGVVTR"
     gene            7320..8129
                     /locus_tag="VN041_07325"
     CDS             7320..8129
                     /locus_tag="VN041_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU28889.1"
                     /translation="MTTLEFKNVTKIYNVRGSGQIRALDDVSFTLRSHQTIGLVGQSG
                     SGKSTIAKILTQLETPTHGEVLLDGTPIPRGGRGLRKYRQQLRMVFQDPFASLNPYHS
                     IRHHVERPLRLDKVVPSGEIDAEVRRLLERVKLDPDAVMERRPHELSGGQRQRVAIAR
                     ALASRPALLVADEPVSMLDVSLRLGVLNLLADLQREQGLGVLYITHDLATARHFSDEI
                     MVLNQGRAVEYGPADDVILNPKDPYTRELRAASPDPEEFFRTAATQAGGAR"
     gene            8126..9244
                     /locus_tag="VN041_07330"
     CDS             8126..9244
                     /locus_tag="VN041_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28890.1"
                     /translation="MSAEQTSAEAVDRASAEQPAVTAATDPTLVGTTATKAEHGGIRV
                     PWRFIGSRVAFYAFTAWAAITINFFLPRLMKGDAVDAYMARSQGQLTPDAEKALRILF
                     GLDKSIPLWQQYLTYWGNLLHGDLGVSISTGMAPVGQAIAAALPWTLGLVGVATVISF
                     IIGTVLGAVIGWKRGSRLDSLVPITTFLGTVPYFWLGLIFIAVFATLLGWFPAGHAYE
                     VGVEPGWNGEFISQVVSHAALPVITIVLVSLGGWVLGMRNMMLTVLDEDYVTVAQAKG
                     MPDDRVLWRYAARNAVLPQIQSFALALGFIVGGTIVMEMVFSYPGIGLLLLSATNAKD
                     FALMQGIFLVLVLAVLIANILADIAYAILDPRTRQTEA"
     gene            9343..10308
                     /locus_tag="VN041_07335"
     CDS             9343..10308
                     /locus_tag="VN041_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28891.1"
                     /translation="MAMFSNAKSATGLVILAIFTLVAILAPLIAPYDPTAQHLDQTLQ
                     PPSLQHLLGTTHIGEDVFSQMVYGTRGVLVVGFLATIMATVVAIVVGVTAGYLRGWKS
                     ESLSALSNVFLVIPGLPLMIIIASQFADPPLWVIAAVLGLTGWAWGARVLRAQTMSLR
                     NRDFIQAARANGEPLHRIILVEMLPNLMAIIASSFVGTMTAAVLGLTTLAFIGVIPVS
                     NLNWGTILFWAQQNNAFPDYWWWYVPAGLCIALVGVALALINFGIDEYVNPRLRSAGE
                     RARAMRKKGLDLNAAVTSVRTPPASARTDEAGAQPGENHAKESVR"
     gene            10305..12581
                     /locus_tag="VN041_07340"
     CDS             10305..12581
                     /locus_tag="VN041_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU28892.1"
                     /translation="MTSHPYQDAALPTADRVEDLLSRMTLEEKVGQMLQLDAREDLDD
                     AVRSRHAGSILHTSPERILLANDLVAQTRLRIPLLVAEDCIHGHSFWPGATIYPTQLG
                     MAASWDADLLERVARATAEEVAVTGIHWTFSPVLCIARDLRWGRVNETFGEDPYLIGE
                     LASAMVRGYQGDGLSDRTAVLATAKHFAGYSETQGGRDASEADISPRKLRSWFLPPFE
                     RVAREGCRTFMLGYQTTDGVPITLNDWLLTDVLKGEWGYTGTLVTDWDNVGRMVWEQH
                     VQPDYAHAAAAATKAGNDMIMTTPRFFEGALAAVEQGLIAPDAFDDAVRRILTLKFDL
                     GLFEDPRMPSEHLDTVVGSRAHADLNLEIARRSLVLLENDGTLPISPAAPGGLISPAA
                     PHSADAAPLRVAVVGPLADDAQNQLGDWAGGSGQAGWLDGQPREMITTVLDGLRAVDG
                     LAVRHARGADILTLVDDPLGATFPDGQPRPPLVVPTPPDQQLLAEAVREAEAADVVVA
                     VVGDRIELVGEGRSTATLELIGGQNALLDALIATGTPVVIVLLASKPLVLPASASQAA
                     AVIWAANPGMQGGRAIAELITGAIEPSGRLPISFARHVGQQPTYYNQIRGQHGHRYAD
                     LTQSPAWAFGEGLSYTTVEYSALSILSTPLGVDDTIVAEVTVTNTGARPMLETVQVYV
                     RDAVTSVSWADKELKTHRQVRLAPGESQRVRIELPVADCTIVDARGRRIIEPGDFEML
                     VGPSSRDEVLLRAGFTVR"
BASE COUNT         2023 a         4456 c         4284 g         2031 t
ORIGIN      
        1 gcggctccgg ctgcgcccga ggctcccgcc gacccggctg cgccgaaggc ctgacgcact
       61 gctccggatg ccggggacgc cgctgacgcg gcatccccgg catccgtcgt ctccgggccc
      121 atctcggcgc tgcagcgtcc agcccattcc gaaccgcacg gcgtaacgtc gagggcatga
      181 gcacggtgcc tgtcgacgag gccgcgatcg ccgaggcgaa gcagctgcgg gatcagttcc
      241 agcgcttcct ccgcgaatac gagttcggca tgcgcgaggt cgagacgaag atctcgatcc
      301 tgcgcgacga gttcacccac atgcacgcct ataacccgat cgagcacgtc tcgagcaggc
      361 tgaagtcgcc agacggcatc gtcgagaagg tcgctcgcaa gggcatcgac cctgacttcg
      421 acgtcatccg gcgggagatc accgacatcg ccggcgtgcg agtcacctgc agtttcgtca
      481 cggacgtgta ccgcctgttc gagctgttca cccggcagga cgacatcacc gtgctgatgg
      541 tgaaggacta catcgccgag cccaagccca acgggtaccg cagcctgcac gcgatcgtcg
      601 aggtgcccgt cttcctgtcc accggtgcga tccccgtgcc cgtcgaggtg cagttccgca
      661 ccatcgcgat ggacttctgg gcgagcctgg agcacaagat ccactacaag ttcgacgggc
      721 gtgttcccgc ccacctggtg cagagcctga ccgaggcggc ggatgccgcc ggcgagctcg
      781 acctgcgcat ggagcgcctg caccgcgagg ccctcgagat caaccatcgg gtgatcgagg
      841 cctgacggcc gacatccggc ccgctcttct gaaggacgat cgacgttctg aaggatgacg
      901 ccgcccagga tctccttcag aacgtcatcc gtccttcaga agcgacgccg gatcagccga
      961 ggaactcccg cgcagccgtc acgagggcct tcacgcccac ctcgatggtc gggtggatca
     1021 ccggggcgaa gaacggcgag tggttggtcg ggatgtcgtg gtcgaccgag tcgttctgca
     1081 cggccgccgc gaagacggcc gggtccacac cgccccagaa ccagaacacc agcggcactc
     1141 ccgcatcccg tgtgaaccag gacacgtcct cgctgccggt gatcaggccg gtgtcgatca
     1201 cggcctgctc accgaacgcg tgctggaacg ccgaggtcgc ccgcgccgtg gcatccgcgt
     1261 cgttgatcgt cgccggaagc gtgtgcagtg tcgagatttc ggggtcgcgt tcggcaccgg
     1321 atgccgcggc ttcggcgcgg atcacgcgct cgaccttctt cagcaccttc tcgcgggccg
     1381 cgtcgtcggg gtagcgcagg ctgaggtcca gggtggcctc ggccgggatg atgttgttct
     1441 tcgtgccggc gtgcacggca ccgaccgtga cgaccgccat gcccgtcgga tcgacctcgc
     1501 gcgagacgat cgtctgcagg cgcatgatcg tcgcggccgc catcacgacc gggtcgatcg
     1561 tggaatccgg ccgcgagccg tgcccgccct ggccgtgcag cacgacgtgc agcccgtcgg
     1621 atgccgacat ctgcggtccg gggcgcaggc cgaccgcgcc tgccgggagc ggcccgacgt
     1681 gctgaccgag cacgatgtcc ggcttcggga accggtccag cgcgccgtcg tcgagcatcg
     1741 cgcgcgcgcc ggcgcccagc tcctccgccg gctggatcac gacgacgagc gttccgtgcc
     1801 actgattctg ctcagccacg agctgctcga tcgcgccgat catggcggtc acgtgcatgt
     1861 cgtggccgca ggcgtgcatc acgccggcct cgttgccgtc cggatcgatg cccgtcgcct
     1921 tcgaggcgta gggcagaccg gtctcctcag tgacgggcag ggcgtccata tcggcacgaa
     1981 cccatacggt cggcccctcg ccgttggcga gcacgcccac gacgcctgtt ccgccgacac
     2041 cttcatgcac ctccagtccg aggtcgcgca gatggccggc ggcgatgccg gcggtacggg
     2101 tctcctggaa cgagagctca ggatgctggt ggaggtcgat gtacagcgct tcgaggtcga
     2161 tgctcatgac gctgagccta gccaccgggg tcggctcgtc ggcaggtgcg cgtggtaggg
     2221 ttgcatgtcc tccgatccgg aggccccggt gatctctcac ctcggcgacc gtcaccatcg
     2281 ccccattcca tctcgcacgc ggacaccgcg cgtgcgaccg caacgttcat ccgctcccgt
     2341 ccgggaggcg cgatccgcgt ccgtcggctt tccgccggga cgcacccggc cgtctcggcg
     2401 gccgaatcaa caggaagaag aaagcacatg gccactggca ctgtgaaatg gttcaactcg
     2461 gagaaggggt tcggcttcat cgcgccggac gacggctccg ccgacctctt cgcgcacttc
     2521 tcggcgatcg catcgtcggg atacaaggaa ctcaccgaga accagaaggt ggagttcgac
     2581 gccgagcgcg gccccaaggg catgcaggcg atgaacatcc gcccgctctg aaccgatgcc
     2641 acgaaccggc ccgcgagatc tcgcgggccg gttctgcgta tccggcgcga ctcggcgggc
     2701 gactctcgtc ccgaaggaca cgcccgccgt cacggccccg gactctggaa ggatggatcg
     2761 gtgacgaagg atgagacccg ccccgccgga ttcggcgccc tgctctggct ggcgatggcg
     2821 acgttctcga tgggcatcga cggctacgtg ctggccggcc tcctcccgca gatcgcgaag
     2881 gacctcgacg tctccgagcc cgccgccggg cagctcgtcg cggtgttcgc gctcaccgcg
     2941 gcgctggcgg gtcccgtgct cagcgcgctg accggcgcct gggagcgcaa ggggatcatc
     3001 tccgcgtcgc tggccgtgtt cgtcgtgggc aatctcgtga tcgcgctgtc cgtgaactac
     3061 accatggcga tgggcggccg gatcctctcc gcgatcggcg gctcgttgct gagtgcggtc
     3121 gtgagcgcct acgtgctggc caagaccgcg ccggagcgcc gggggcgtgc cctgtcgttc
     3181 gtgctcggcg gcttcctcgc agcgaccgcc ttggggtccc gatcggactc gtcatcggcc
     3241 aggaggactg gcggctgccg ctgtacctgg tcaccggggt cggcgccgtc gcgctgatcg
     3301 gcatcctggt gaacgttccg agactgcatc tgccggcgtt gtcgctgcgc gagacgctga
     3361 agccgctggc ccgtccggct ctcgtgggct cgatcctcgt ggccaccggg atcatgtgcg
     3421 cgagttacct gtgcttcacc tacgccaccc tgatcttcgg gccgcgggtc ggcgaggggc
     3481 ccgcgatcat cgcggccatg ttcggctacg gcgtcgtcag cctgttcggc aacatcgtct
     3541 cggggcgcgc caccgaccgg ttcaacccgg tgcacgtgat ctcgttgatc gtcggcatcc
     3601 tgatcgtcgt cgcgctgctg ggcaccgcgg ggctgctgct ccccggcgcg gccggtgcgg
     3661 tcgcggcgtt cgcgtggttc ttctgctgcg cgttcttcaa cggcggctct ggggtcgccc
     3721 tgcagtcccg gctgggtgac ctggctcccg acgcgatgtc gctggtgctg gcgctgaacg
     3781 gcagcggcat gcagctcggc tcggcgctgg gagggatgct gggtggcgcg ctgctggcat
     3841 ccggacttgc cgccgacggg ctggtgccgg catccgccgt ggtgctcgcc gcgacgatga
     3901 tcctgcacct gatcgtgtcg cgggcgaagc ccgcggcgcg cacggcctga tcagcggcgg
     3961 taggactcca gcgggggtcc ggggtgcagc acaccgttga cgatcgcggt gatcgcgaac
     4021 tcgctcgact cgccgaggcc gacgatctcg ggatgcagca gccattgcag ctgcaggccg
     4081 tccatgactg cgaggatggc ggcggcggcg tgggcgatcg tgtcggtgtc cgtgacgccc
     4141 tccgccctgc acagcacctc gaaggcctcc gtgacctcgc ggcgcagcgt gatgtaccgg
     4201 tcctcgaagt actcccgtcc cggatggtcg tcggtcaccg attcggacga cagcacggtg
     4261 tacgcctgca cgatgccggg gcgacgctcg ttcgcgaacg cggtgcgcac gaggtgcagg
     4321 aacagctcga gcccgcccgg gatgtgcttg tcgtccagat cggcgacgtc ggcctggtcg
     4381 cgatacgcca gcacctcgag cagcagtctc tgcttggagc cgaagtggtg cagcacgccg
     4441 gcgtgggtca gtccgacctg ctcggccaca tccgcgagcg tgccgttggt cgagcccttg
     4501 ctcccgaaga tctcgaccgc cgcgttcagg atctcgatgc gcttgtgctg cgccgcccgg
     4561 gtgcggggcg tgtcctgtgt ggccttcggc acgatgtcct cctcagggcg agtgtaacgg
     4621 tctgataatg ctctcttgaa agcttactta ctagtaggta acctaccctc aattcacttt
     4681 ctcgccagta agtgaaactc ggaggccgag cgcagacgct cgcccctcgc accaggacac
     4741 cgtgccctag gagaagcaat gaagctcaag aagtacttga tcgcaggcac tgcgatcgcc
     4801 gcactgagtg cgctcgccct gaccggatgc tcgtcgagcg gctcggccgg gtccggcggt
     4861 ggcggcacgt cgctgaccgt cgcgaagccg gacggcgccg gtgtgctctc gacgcagatc
     4921 aacaacccgt tcatcggcac cggctccggc atgtcgctgg cgtacgaccg gatgatctac
     4981 gagcccctcg cgatggtcaa cccggtcggc aagaacgaga ccacgccgtg gctcgcctcg
     5041 aaggtggagt ggaacagcga ctacacgcag ctcacggtca ccccgcgcaa ggacgtgaag
     5101 tggaacgacg gcaagccgtt caccgccgat gacatcgtct tcaccttcac cctcatcaag
     5161 aacacccccg ccctcgatct cggcggcctg aagctgagcg gcatcgagaa ggatggcgac
     5221 gacgtcgtga tgacgttcag cgagtcgaag ttcgtcaagc agggagacgt cctccaggtg
     5281 ccgatcgtcc ccgagcacca gtggaagagc atcgccgacc cgaccaagga cccggtcaag
     5341 gatgccatcg gcaccgggcc ctacacgatc aagagcttct cgtcgcaggg cgtcgtgctc
     5401 agcgcgcgca gcgattactg gggcggcaag gtcccggtgg cgaccctcaa gtacctcgag
     5461 tacaacgaca acaccggcct gctccgcgcg ctgcagagca aggaggccga ctgggcgcag
     5521 atcttcatca ccgactacca gaccaacttc gtcgacaagg acccgaagca caacgtgttc
     5581 tggggcgcca acgtcctgag tccggacatg atcctggtca acacaacgaa ggcccccttc
     5641 aacgacgtcg cgttccgcaa ggccgtcaac atggtcgtcg accgcaaggc gcacgccgag
     5701 aaggcgcgca gcaacgccgg tccggagctg aagaacgtca ccggcatccc gcagcccacc
     5761 ggcgaccagt acatcgcccc cgagtacaag gacaagaact tcaccgtcga cgtcgacggt
     5821 gccaagaaga tcctcaccga cgccggctac acctggaagg gtggcgcact gatcgatccg
     5881 tccggcaacc cggtctcgtt caccctgcag gacccgcagg gatggaacga ctacgtcacg
     5941 ggcatccagc tggtcgcgac ccagatcaag agcaccctcg gcgccgacgc gaaggtcgcg
     6001 accccggacg ccgacacgtg gttcagcaac atggcgaccg ggaacttcga tgcggcactg
     6061 cactggtcgg gatccggatc caacccgtgg cacatctacg acgacgtcat gaacggcgcg
     6121 tatctcgagc aggcgtcggg cggcaaggtc tccgacaact tcggacgcta cgacaacccg
     6181 gaggcgaccg ccctgctgaa gcagtacgcc caggcatccg acgacgcagc ccgcaccgac
     6241 gcgctcaaca agatccagaa gatcttcgtc gaggacgtcc cggccatccc gatcggcacc
     6301 cacccccagc tggcggagta caacacccgc acgttcacgg gctggccgag cgagagcgac
     6361 cagtacgcga cggccgaccc gactcagccg tccgccgtcc aggtgctgat gaacctgaag
     6421 ccggtcaagt agtcgtcagt gcaggtccgg ggcgggctct cccgccccgg acctgcaccg
     6481 agccgaaggt ccttcctcat gacagatccc ctcctctcga tacgcgactt ctcggtcgtg
     6541 tacgacgtcg atccgccggt cgaggcggtc aagcacgtca ccctcgacat ccgccgcggt
     6601 gagatcatcg gtctcgccgg cgagagcggg agcggcaaga ccacgctcgc ctacggcgtg
     6661 cagagactcc tcaagccccc ggccgtgatc accagcggca gcgtcgtctt ccacgacgag
     6721 gacggcgccg acatcgacct gcacgcgctc gacgtggagc agatgcggcg cttccgctgg
     6781 gacaaggtct ccatggtgtt ccagggcgcc atgaacgcgc tcaaccccgt ggcgaccatc
     6841 ggctcgcagc tcgacgacgt gttcgtggtg caccggcccc gcatgggacg acgtgagcgc
     6901 cgtgaggcgg tggacgaact gctggagatc gtgaaggtgg gcgctcagcg tgcgcggtcg
     6961 tacccgcatg agctctccgg cggcatgcgc cagcgcgtca tgatcgcgat ggccctcgcc
     7021 ctgcgaccgc agctgatggt catggacgag cccacgaccg ctctggacgt gctggtgcag
     7081 cgggagatcc tcaagcagat ctcccagctg cgccatgagt tcgggttctc ggtggtgttc
     7141 atcacccatg acctgcccct gctgctggag atcagcgacc gcatcgccgt gatgcgcgag
     7201 ggcgagatcg tcgagctgtc ggacgcgagg gagctgtgga cgaacccccg gcacgagtac
     7261 acgcagaacc tgctgcgctc gttcccccgc ctcacgggag agagaggagt ggtgacgcga
     7321 tgacgaccct cgagttcaag aacgtcacga agatctacaa cgtccgcgga tcggggcaga
     7381 tcagggccct cgacgatgtg agcttcacgc tccgctcgca tcagacgatc ggactggtag
     7441 gacagtccgg cagcggaaag tcgacgatcg cgaagatcct cacgcagctc gagacgccca
     7501 cgcacggcga ggtgctgctc gacggcacgc cgatcccgcg cggcgggcgg gggctgcgca
     7561 aataccggca gcagctgcgc atggtgttcc aggacccgtt cgcctcgctg aacccgtacc
     7621 actccatccg ccaccacgtc gagcgtcccc tccgcctgga caaggtcgtc ccgtccggcg
     7681 agatcgacgc ggaggtgcgc cggctgctgg agcgcgtgaa gctcgatccg gatgccgtga
     7741 tggagcgacg cccgcatgag ctgtccggcg ggcagcgcca gcgcgtggcg attgcccgtg
     7801 ccctggcatc ccggcccgcg ctgctggtcg ccgacgagcc ggtctcgatg ctcgacgtgt
     7861 ccctgcgcct gggcgtgctc aacctgctcg cggacctgca gcgcgagcag gggctcggcg
     7921 tgctctacat cacgcacgat ctcgccacgg cccggcactt cagcgacgag atcatggtgc
     7981 tcaaccaggg cagggcggtc gagtacgggc ccgcagacga cgtgatcctg aacccgaagg
     8041 acccctacac gcgcgagctg cgcgccgcat cccccgaccc ggaggagttc ttccggaccg
     8101 ccgcgacaca ggcaggaggc gcacgatgag cgcggagcag acatcggcgg aggcggtgga
     8161 ccgggcatcc gcggaacagc ccgccgtcac ggccgcgacc gacccgacgc tggtcgggac
     8221 gacggcgacg aaggccgagc acggcggcat ccgcgtgccc tggcggttca tcggcagtcg
     8281 cgtcgcgttc tacgcgttca cggcgtgggc cgcgatcacc atcaacttct tcctcccccg
     8341 gctgatgaag ggcgacgcgg tcgacgcgta catggcgcgc agtcagggac agctcacgcc
     8401 ggatgccgag aaggcgctgc gcatcctgtt cgggctggac aagtccatcc cgctctggca
     8461 gcagtacctc acgtactggg gcaacctgct gcatggcgat ctgggcgtct cgatctccac
     8521 gggcatggcg ccggtgggtc aggccatcgc cgcggcgctg ccgtggacgc tcgggctggt
     8581 gggcgtggcg accgtgatct cgttcatcat cggaaccgtg ctcggcgccg tgatcggctg
     8641 gaagcggggc agcagactcg actcgctggt gcccatcacg acgttcctcg gcaccgtgcc
     8701 ctacttctgg ctcggcctga tcttcatcgc ggtgttcgcc accctgctcg gatggttccc
     8761 cgcggggcac gcgtacgagg tgggtgtcga gcctggctgg aacggcgagt tcatctcgca
     8821 ggtcgtctcg cacgccgcgc tccccgtgat cacgatcgtg ctcgtctccc tgggtggctg
     8881 ggtgctcggg atgcgcaaca tgatgctcac ggtgctcgac gaggactacg tcaccgtcgc
     8941 acaggcgaag ggaatgcccg acgaccgcgt gctgtggcgc tatgccgctc ggaacgccgt
     9001 gctgccccag atccagagct tcgccctggc cctcgggttc atcgtcggcg gcacgatcgt
     9061 gatggagatg gtgttctcct acccgggcat cggcctgctg ctgctcagtg cgaccaacgc
     9121 caaggacttc gccctgatgc aggggatctt cctggtgctg gtcctcgccg tgctgatcgc
     9181 caacatcctc gccgacatcg cctacgcgat cctcgacccc cgcacccggc agacggaggc
     9241 ctgatcatga ccgctcccac gcagaacctg atcgcccagc aggcgttcac cgcgcccccg
     9301 gcgccccggc ggaagacgtt ctggagtcag ctcggcgcat cgatggcgat gttcagcaac
     9361 gccaagtcgg ccacggggct cgtcatcctc gcgatcttca cgctggtcgc gatcctcgcg
     9421 ccgctgatcg ccccgtacga cccgaccgcg cagcacctcg accagacgct gcagccgccg
     9481 tcgctccagc acctgctggg gaccacccac atcggcgagg acgtgttcag ccagatggtc
     9541 tacggcacgc gcggcgtgct cgtggtcgga ttcctggcga cgatcatggc cacggtggtc
     9601 gcgatcgtcg tcggcgtgac ggccggctat ctgcgcggct ggaagagcga gtcgctgtcg
     9661 gcgctgtcca acgtcttcct ggtcatcccg gggctgccgc tgatgatcat catcgcctcg
     9721 cagttcgccg acccgccgct gtgggtcatc gcggcggtgc tggggctgac cgggtgggcg
     9781 tggggcgccc gggtgctgcg cgcgcagacc atgtcgctgc gcaaccgcga cttcatccag
     9841 gccgccaggg ccaacggcga gccgctgcac cgcatcatcc tggtggagat gctgccgaac
     9901 ctcatggcga tcatcgcctc cagcttcgtc gggaccatga ccgctgcggt gctggggctg
     9961 acgacgctcg ccttcatcgg ggtgatcccg gtgtcgaacc tgaactgggg caccatcctg
    10021 ttctgggcgc agcagaacaa cgcgttcccg gactactggt ggtggtacgt gcccgccggc
    10081 ctgtgcatcg cgctggtcgg tgtcgccctg gcgctgatca acttcggcat cgacgagtac
    10141 gtcaacccgc ggctccgctc cgcgggtgag cgcgctcgtg ccatgaggaa gaagggcctg
    10201 gatctgaacg ccgccgtcac ctcggtgcgg acgccgccgg catccgcccg caccgacgag
    10261 gccggagcgc agcccggcga gaaccacgcg aaggaatcgg tacgttgacc tcccacccct
    10321 atcaggacgc cgctctgccg accgccgatc gggtggagga tctgctcagc aggatgaccc
    10381 tcgaggagaa ggtcgggcag atgctgcagc tggacgcccg cgaggacctg gacgatgcgg
    10441 tgcgttcccg ccacgcggga tcgatcctgc acacctctcc ggagcggatc ctgctggcga
    10501 acgacctcgt cgcgcagacc cggctccgca tcccgctgct ggtggcggag gactgcatcc
    10561 acggccactc gttctggccc ggcgcgacga tctaccccac ccagctcggc atggcggcgt
    10621 cctgggacgc cgatctgctc gagcgcgtcg cgagggccac cgctgaggag gtcgccgtca
    10681 cgggcatcca ctggactttc tccccggtgc tgtgcatcgc ccgcgatctg cgctgggggc
    10741 gggtgaacga gaccttcggc gaggacccgt atctgatcgg cgagctggcg tcggcgatgg
    10801 tacgcggcta ccagggggac ggcctgtcgg atcgcacggc cgtcctcgcc acggccaagc
    10861 acttcgccgg ctactccgag acccagggcg ggagggatgc cagcgaggcc gacatctcac
    10921 cccgcaagct ccgctcgtgg ttcctgcctc cgttcgagcg cgttgcccgg gagggctgcc
    10981 gcacgttcat gctcggctac cagaccacgg acggcgtgcc gatcacgctg aacgactggc
    11041 tgctcaccga cgtcctcaag ggcgagtggg gctacaccgg cacgctggtc accgattggg
    11101 acaacgtcgg gcgcatggtg tgggagcagc acgtgcagcc cgactacgcg cacgccgccg
    11161 cggcggcgac gaaggccggc aacgacatga tcatgacgac gccacggttc ttcgaaggcg
    11221 cgctcgccgc ggtggagcag gggctgatcg caccagacgc gttcgacgac gccgtgcgcc
    11281 gcatcctgac tctcaagttc gacctcgggc tgttcgagga cccgcggatg ccgtcggagc
    11341 acctcgacac ggtggtcggc agccgggcgc acgccgacct gaacctcgag atcgcgcggc
    11401 gctcgctcgt cctgctcgag aacgacggga ctctaccgat ctcgccggcc gcgcctggtg
    11461 ggctgatctc gccggctgcg cctcactctg cggatgccgc acccctccgg gtcgcggtgg
    11521 tcggcccgct cgcggacgac gcgcagaacc agctcggcga ctgggccggc ggctcaggtc
    11581 aggcgggctg gctggacggg cagccgcggg agatgatcac caccgtgctc gacgggctcc
    11641 gcgccgtcga cggcctcgcc gtacgtcatg cgcggggcgc ggacatcctc actctggtgg
    11701 acgacccgct cggcgcgacg ttcccggacg gacagccgcg cccgccgctg gtggtgccca
    11761 cgccgcctga tcagcagctg ctcgctgagg ccgtacgcga ggcggaggcc gcggacgtcg
    11821 tcgtcgccgt cgtgggcgac cggatagaac tggtgggcga gggcaggtcc acggcgacgc
    11881 tggagctcat cggcgggcag aacgcgctgc tggacgcgct gatcgccacc ggaacgccgg
    11941 tcgtgatcgt gctgctggcc tccaagccgc tggtgctgcc ggcatccgcg tctcaggccg
    12001 ccgcagtgat ctgggcagcg aaccccggca tgcagggcgg tcgcgcgatc gccgagctga
    12061 tcaccggcgc catcgagccc tccggacggc ttcccatctc gttcgcgcgt cacgtcggcc
    12121 agcagccgac gtactacaac cagatccggg gccagcacgg ccaccgctac gccgatctga
    12181 cccagtcacc cgcctgggcc ttcggtgaag ggctttcgta cacgaccgtc gagtactccg
    12241 ccctgagcat cctgtccacc ccgctgggcg tcgacgacac gatcgtggca gaggtcacgg
    12301 tgaccaacac gggcgcccgc ccgatgctcg agaccgtgca ggtctatgtg cgcgatgccg
    12361 tgaccagcgt cagctgggcg gacaaggagc tgaagaccca tcggcaggtg cggctggcgc
    12421 cgggggagtc gcagcgcgtg cgcatcgagc tgcccgtcgc ggactgcacg atcgtcgatg
    12481 cccgcggtcg gcggatcatc gagcccggcg acttcgagat gctcgtcggg ccgtcgtccc
    12541 gcgacgaagt gctgctgcgg gccgggttca ccgtgcgctg accctgcgcg gtcgtcgccg
    12601 cgcgccgcgc cgcgctctcg catgctgtgc gcttggtgcg ggaatgtgcg ctttgcgcgg
    12661 acggatgccg tgattcgatc cgcgggaagc gcacatctcc gcgcgaagtg ctcggcgcgg
    12721 ttgcgtctgc gccgccgcaa gcaggaccag cgcgctacac ccgcgccgtc gtgccgcgga
    12781 cgatcagctc gtag
//
LOCUS       DATMSX010000240         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_31877,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000240 DATMSX010000000
VERSION     DATMSX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31877"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VN041_07345"
     CDS             complement(<1..396)
                     /locus_tag="VN041_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HWU28893.1"
                     /translation="MADVTKRMLDLLSALQSGRGYAGPELAALLGVSTRTLRRDVDRL
                     RDYGYPVHTRPGPAGGYRLIAGGSMPPLLLDDDEAVAAQVALTVVAAMQDSAPGSISD
                     STARAQAKLDQVLPERLRPHAAALRDALDI"
     gene            460..2715
                     /locus_tag="VN041_07350"
     CDS             460..2715
                     /locus_tag="VN041_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWU28894.1"
                     /translation="MTDTISIRGARTNNLRGIDIDIPRGELVVFVGLSGAGKSSLVFE
                     TIAAEAGYQLNETFPPFARNRLPKWKRPTVDAISGISPVVVIDQKRIGGNARSTVGTV
                     TDAWTHLRLLFSRLSTPHVGESTSFSFNDPSGMCPRCAGLGEVVTSAVDRFLDLDRSL
                     EQGAILVPGFGHGGYFYRLYADAGSFDLGTPLREWTEDERAALLYGGEAMARLGRGAP
                     RDYEGVVPRFERIHLHTGDHVSERKAAVVREFTRSEPCPDCGGDRLNEAARTATVHGR
                     TIVELARQEVTELADLVRGIDEPAVAPVVEALVQRLDALDSIGLGYLNLGRATPTLSG
                     GESQRIKTVKHLGSSLTQMLYVFDEPTVGLHPADVDAMVGLLRELRDKGNTVLVVEHD
                     PAVMASADQVIEIGPSAGEGGGRLVFQGGYEELLHADTATGRALRAPLPDPGDRKPPT
                     GWIGVRRARRNNLHDLSLDLPTGVLTVLTGVAGSGKSSLAAELVAQHHAVVIDQRPVA
                     GNRRSTPITYTGIATPLRRMFADAHGVSPGLFSANSEGGCLACGGLGVIHTDLAFMEG
                     QETPCEACRGRRFRPEVLALTVDGLSIADVDELTVAEAAVRLPSPRIRTALAHLDGIG
                     LGYLRLGQPLNTLSGGESQRVKIAAELRDRTEPALYVLDEPTTGLHASDVDTLFRMLV
                     ALRDRGHTVVVIEHNLAVIQRADWLIDLGPGAGRHGGRLLYQGPVAGVTGTPTARALQ
                     QEQSRRASSAV"
     gene            2844..3605
                     /locus_tag="VN041_07355"
     CDS             2844..3605
                     /locus_tag="VN041_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28895.1"
                     /translation="MTDAPAVTVEALVVRRGRRTLFDGLDVSIPHGEITGLLGPSGCG
                     KTTLLRAIVGVQKVKGGMVTVLGQPAGSRGLRERVAYGTQGAAVYGDLTVRQNLQYFA
                     SVLGVPRGDVDRVLDEVGLRDHAKQQVADLSGGQAGRVSLGAALLGSPELVVLDEPTV
                     GLDPVLRSELWGMFRRIADAGTTLIVSSHVMDEARRCDRLLLMRDGRLIADTTPAALL
                     EDTGEDDAEDAFLALIARDEREHPPTRRSQRGAAS"
     gene            3602..4336
                     /locus_tag="VN041_07360"
     CDS             3602..4336
                     /locus_tag="VN041_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU28896.1"
                     /translation="MNPRRMFATAGRVFGQLRHDPRSIALMLVAPSLLVGLFAWLFAD
                     QDGVFDRFGGAILALFPFIVMFLITSITTLRERRSGTLERLMTTPLGKADFILGYALA
                     FGLMAILQAVITVAFAVYACGLTVDGPLWQLGAVAVLDAVLGTALGLLASAFAQTEFQ
                     AVQFMPVLVFPQIILGGLFMPRDRMPDVLYAISDWLPLSHAIDAIQAVTKGDEGWDVG
                     APLLIVAAFAVGCLVLAPLTLSRRTP"
     gene            complement(4456..>5036)
                     /locus_tag="VN041_07365"
     CDS             complement(4456..>5036)
                     /locus_tag="VN041_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HWU28897.1"
                     /translation="FIWRDAADELRRRLALLHRELGAPWPDVSDAGLLAAIDAWLTPE
                     LDALAGGTPADRLDLASALRRLLPWPEAVRFDELAPERLEVPSGSRIRVAYPPLDDPT
                     ARPVVAVKLQECFGWAETPRLVDGRVPVLFHLLSPAGRPLAVTDDLASFWSGPYAQAR
                     AEMRGRYPKHPWPEDPWAAAPTRHTKNRAPRG"
BASE COUNT          732 a         1717 c         1834 g          753 t
ORIGIN      
        1 gatgtccagg gcatcgcgca gggcggccgc gtgtggccgc agccgctcgg ggaggacctg
       61 gtcgagcttg gcctgggcgc gtgcagtgct gtcggagatc gatcccggag cagagtcctg
      121 catcgccgcg acgacggtca gcgcgacctg cgcggcgacg gcttcgtcat cgtcgagaag
      181 gagcggcggc atggatcctc cggcgatcag ccgatagccg ccggccggcc cgggcctggt
      241 gtgcaccgga tacccgtagt cgcgcagccg atcgacgtct cggcgcagcg tgcgcgtgct
      301 cactccgagc agcgcggcga gctccgggcc ggcgtacccg cgcccgctct gcagcgcgga
      361 gagcagatcc agcatccgct tcgtgacgtc ggccatgaca acgaggatag gtcgaattgc
      421 ggacagaatc aggccgcaat gcggcctagc gtgtgcgaca tgaccgacac gatcagcatc
      481 cgaggagcgc gcacgaacaa cctgcgcggc atcgacatcg acatcccgcg cggcgagctc
      541 gtcgtgttcg tcggcctgtc cggcgcgggc aagtcctcgc tcgtcttcga gacgatcgcc
      601 gccgaggccg gctatcagct gaacgagacg ttcccgccgt tcgcccgcaa ccgcctgccg
      661 aagtggaagc gtccgacggt ggacgccatc tccggcatct cgccggtggt ggtgatcgac
      721 cagaagcgca tcggaggcaa cgcgcggtcg acggtcggca ccgtcaccga cgcctggacc
      781 cacctgcgtc tgctgttctc ccggctcagc acgccgcacg tcggggagtc gacgtcattc
      841 tccttcaacg acccctccgg catgtgccct cgctgtgccg gtctcggtga agtcgtcacc
      901 agcgccgtgg accggttcct cgacctcgac cgctcgctcg agcagggcgc gatcctcgtg
      961 ccggggttcg gccacggcgg ctacttctac cgtctgtatg cggacgccgg cagtttcgac
     1021 ctcggcactc cgctgcggga gtggaccgag gacgagcgcg cggccctgct gtacggcggt
     1081 gaggcgatgg ccaggctcgg ccgtggcgcg ccgagggact acgaaggagt ggtgccgcgg
     1141 ttcgaacgga tccatctgca caccggtgat catgtctcag agcgcaaggc cgcggtcgtg
     1201 cgcgagttca cccggtcgga gccctgcccg gactgcggcg gggatcggct gaacgaggcg
     1261 gcacgcacgg cgaccgtgca cggccgcacg atcgtcgagc tcgccaggca ggaggtcacg
     1321 gagctggccg atctcgtgcg cgggatcgac gagccggcgg tggcacccgt ggtcgaggca
     1381 ttggtgcagc ggctcgacgc actggactcc atcggtctgg gctatctgaa tctcggacgg
     1441 gcgacgccga ccctctcggg tggggagtcg cagcggatca agaccgtgaa gcatctcggc
     1501 tcgagcctga cccagatgct ctacgtcttc gacgagccga ccgtcgggct gcatccggca
     1561 gatgtcgacg ccatggtcgg actgctgcgt gagctgcgcg acaagggcaa cacggtgctc
     1621 gtggtcgaac atgaccccgc ggtgatggca tccgcggatc aggtgatcga gatcggcccg
     1681 tccgcagggg agggcggcgg tcgtctggtg ttccagggag gatacgagga gcttctgcac
     1741 gcggacactg ccaccggccg cgcactgcgc gcgccgctgc cggatcccgg ggaccggaag
     1801 ccgcccacgg ggtggatcgg cgtgcggcgt gctcggcgca acaacctgca cgacctgtcg
     1861 ctggatcttc ctaccggggt gctgacggtc ctcaccgggg tcgcgggctc gggcaaatcc
     1921 agcctcgcgg ccgaactcgt ggcgcagcac cacgcggtcg tgatcgacca gcgcccggtg
     1981 gcaggcaatc ggcggtcgac gccgatcacc tacaccggaa tcgcgacacc gctgcgccgc
     2041 atgttcgccg acgctcatgg cgtctccccc ggcctgttca gcgcgaactc ggaaggcgga
     2101 tgcctggcat gcgggggact cggagtgatc cacaccgatc tggcattcat ggaggggcag
     2161 gagacgccct gcgaggcgtg caggggacgc cgcttccgac ccgaggtgct cgcgctgacc
     2221 gttgacgggc tgagcatcgc agacgtcgac gaactgacag tggcggaagc cgccgtgcgg
     2281 ctgccgtcgc cgcgcatccg cactgcactc gcgcatctcg acgggatcgg actcggctac
     2341 ctgcgactgg ggcagccgct gaacacactc tcgggcggag agagccagcg agtgaagatc
     2401 gcggccgagc tgagggatcg gaccgagccc gcgctgtacg tgctcgacga gccgacgacg
     2461 ggactgcatg cctcggatgt cgacacgctg ttccggatgc tcgtcgcgct gcgagaccgg
     2521 gggcacacgg tcgtcgtgat cgaacacaac ctggccgtga tccagcgggc ggactggctg
     2581 atcgacctcg gccccggtgc ggggcggcac ggcggccgtc tgctgtacca ggggccggtc
     2641 gccggagtca cggggacgcc gactgcgcgg gcgctgcagc aggagcagtc ccgcagggca
     2701 tcatcggccg tctgagccgg atccggcggt gagacccgtg cgaccgcgcg gatcgagcgg
     2761 cggcgcggtc ctggaacgga aggacgacga gccgctgcgg cgggtccctg gtgcgtgcgc
     2821 cgtcgacggc gcacaatcaa cacatgacgg atgctccggc ggtgacggtg gaggccctcg
     2881 tggtgcggcg cggacgccgc acccttttcg acgggctgga cgtgtcgatt ccacacggcg
     2941 agatcaccgg gctgctcggg cccagcggat gcggcaagac gacgcttctg cgcgcgatcg
     3001 tgggagtgca gaaggtgaag ggcggcatgg tgaccgtact cggccagccc gccgggtcgc
     3061 gagggctgcg cgagcgggtc gcctacggaa cccagggtgc cgcggtgtac ggcgacctga
     3121 ccgtgcggca gaacctgcag tacttcgcgt ccgttctcgg tgtgccacgc ggtgacgtcg
     3181 accgagtgct cgacgaggtg ggcctgcgcg atcatgcgaa gcagcaggtc gccgacctca
     3241 gcggcgggca ggccgggcgg gtctcgctcg gcgccgccct gctgggcagc cccgaactgg
     3301 tcgtgctgga cgaaccgacg gtgggcctcg acccggtgct gcgcagcgag ctctggggca
     3361 tgttccggcg aatagcggat gccgggacga cgctcatcgt ctccagccac gtgatggatg
     3421 aagcccgccg ctgcgaccgc ctgctgctca tgcgcgacgg ccggctgatc gccgacacca
     3481 cgccggcggc gctgctcgag gacaccggcg aggacgatgc cgaggacgcg ttcctcgccc
     3541 tcatcgcgcg cgacgagcgg gagcatccgc ccacgcggcg ctcccagcgg ggagcggcgt
     3601 cgtgaacccg cgccggatgt tcgcgaccgc ggggcgcgtc ttcggtcagt tgcgccacga
     3661 tccgcgttcg atcgcgctca tgctcgtggc gcccagcctg ctggtcggcc tgttcgcctg
     3721 gctgttcgcc gatcaggatg gggtgttcga caggttcggt ggcgcgatcc tggcgctgtt
     3781 cccgttcatc gtgatgttcc tgatcacgtc gatcaccacg ctgcgcgagc ggcgctccgg
     3841 cacgctcgag cggctgatga ccacgccgct cggcaaggcc gacttcatcc tcggctacgc
     3901 gctcgcgttc gggctcatgg cgatcctgca ggcggtcatc acggtcgcgt tcgccgtgta
     3961 cgcgtgcggg ctgaccgtcg acgggccgct gtggcagctg ggcgcggtgg cggtgctgga
     4021 tgccgtgctc ggcaccgccc tcggactgct ggccagcgcg ttcgcgcaga ccgagttcca
     4081 ggccgtgcag ttcatgccgg tgctggtgtt cccgcagatc atcctcggcg gcctgttcat
     4141 gccgcgcgat cggatgcccg acgtgctcta cgccatctcc gactggttgc cgctcagcca
     4201 tgcgatcgac gccatccagg cggtcacgaa gggcgacgag ggctgggacg tcggcgcgcc
     4261 gctgctgatc gtcgcagcct tcgcggtcgg atgcctggtg ctggcgccgc tcaccctgag
     4321 ccgccgcacg ccgtgaggtc gtccgatccc gagcatccgt cgcccgatct gcggacgaag
     4381 tcccgattcc cggacggatg cggtcgatcg gcccactgat cgggacaacg tccgctgacc
     4441 ggggcggtga gtggctcagc cgcgcggggc gcggttcttc gtgtgtcggg tgggggcggc
     4501 cgcccaggga tcctctggcc acggatgctt ggggtaccgc ccccgcatct cggcgcgcgc
     4561 ctgcgcgtac gggcccgacc agaaggaggc cagatcgtcg gtgacggcga gtggccgccc
     4621 ggcgggggag agcaggtgga acagcaccgg cacgcggccg tcgaccagtc gcggtgtctc
     4681 ggcccagccg aagcactcct gcagcttcac cgcgaccacc ggccgcgccg tcgggtcgtc
     4741 gagcggtggg taggcgacgc ggatgcggga gccactcggc acctcgaggc gctccggcgc
     4801 cagctcgtcg aaccgcaccg cctctggcca cggcagcagg cgtcgcagcg cggatgccag
     4861 gtccagccga tccgccgggg tccctccagc gagggcatcg agctcgggcg tcagccaggc
     4921 gtcgatggcc gcgagcaggc cggcatccga cacatcgggc cagggcgcgc ccagctcccg
     4981 atgcagcagt gccaggcggc gccgcagctc gtccgccgca tcccgccaga tgaaca
//
LOCUS       DATMSX010000241         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_22228,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000241 DATMSX010000000
VERSION     DATMSX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22228"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1878
                     /locus_tag="VN041_07370"
     CDS             <1..>1878
                     /locus_tag="VN041_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HWU28898.1"
                     /translation="PDRLVLDLDPGPGVGLAECAEVARIARGILSGMGLEPMPVTSGS
                     KGIHLYARLPTADDGTGLQSSDEVSAVAKELARLIEADHPDLATRTMAKSARGGKVFI
                     DWSQNSASKTTIAPYSLRGRARPWVAAPRTWEELDDPALRQLEFDEVLERVDAGIDPL
                     AALAPPSTALTSYLAKRDAAKTPEPMPTTAVAGAPGAPRFVIQEHHASRLHYDLRIER
                     DGVLISWAVPKGVPETPDRNHLAVMTEPHPLEYLTFEGEIPRGEYGAGSMTVWDTGTV
                     ALEKWRDDEMIGTFTGQAGGRLGSARLALIRTEGEGEKSQWLLHRMKDGGGSPPAPER
                     RRGEPSPERRRRLLTREAAIPAEAASSAECPIRPMLSETGSPGLARGYDWAEIKWDGV
                     RAIGTWADGRLTLRARSGTDITARYPELTADGAPHLPASDAVIDGEIVAFDAQGRPSF
                     TRLQNRMHLTRGREIEREVVRTPVVYLLFDLMRLDGHDLTRMPLRQRRELLEQLASDL
                     DAPVQVPPVFDDLDAAMEMSGRYGLEGVVAKDPGSPYRPGARSSSWLKLKHTRTQEVV
                     VVGIRPGQGNRREGIGSLLLAVPDDDGALRYVGRVGTGFTDRMLSDLRARLEPLRTGS
                     P"
BASE COUNT          286 a          679 c          673 g          240 t
ORIGIN      
        1 ggcccgaccg gctggtgctg gacctcgacc ccggccccgg agtggggctc gcggagtgcg
       61 cggaggtggc gcggatcgcc cgcggcatcc tctccggcat gggactggag ccgatgcccg
      121 tgaccagcgg cagcaagggc atccacctgt acgcccgtct gccgaccgcc gacgacggga
      181 ccggcctgca gagcagcgac gaggtgtccg ccgtcgcgaa ggagctcgcc cggctcatcg
      241 aggcggatca tcccgacctc gccacgcgaa ccatggcgaa gtcagcgcgc ggggggaagg
      301 tcttcatcga ctggagccag aacagcgcct ccaagacgac catcgcgccc tactcgctgc
      361 gcgggcgcgc gcgcccgtgg gtcgctgccc cgcgcacctg ggaggaactg gacgatcccg
      421 cactccgcca gctggagttc gacgaggtcc tggagcgggt ggatgccggg atcgacccgc
      481 tcgcagccct cgcgccgccc agcaccgcgc tcacttccta cctcgccaag cgggatgccg
      541 cgaagacgcc cgagccgatg ccgaccacgg ccgtcgccgg cgcccccggt gcaccgcgtt
      601 tcgtgatcca ggagcaccac gcgagccgcc tgcactacga cctgcgcatc gagcgcgacg
      661 gcgtgctgat cagctgggcg gtgcccaagg gcgtgccgga gacgccggat cgcaaccacc
      721 tggcggtgat gaccgagccg catccgctgg agtacctcac cttcgagggc gagatcccgc
      781 gcggggagta cggcgccggt tccatgaccg tgtgggacac cggcaccgtg gcgctggaga
      841 agtggcgcga cgacgagatg atcggcacgt tcaccgggca ggcgggcggg cggttgggat
      901 cggcgcggct cgctctgatc cgcaccgagg gcgagggcga gaagtcgcag tggctgctgc
      961 accggatgaa ggacggcggc ggtagccctc ctgccccgga gcggcgacga ggggaaccct
     1021 cccctgaacg ccgccgtcgt ctcctcacgc gagaagctgc catccccgcg gaagcggctt
     1081 cgtccgctga atgccccatc cggcccatgc tctccgagac gggctccccc ggcctcgccc
     1141 gaggctacga ctgggcggag atcaaatggg acggcgtccg cgcgatcggg acctgggccg
     1201 acggcaggct caccctccgc gcccgcagcg gcactgacat caccgcacgc tatccggagc
     1261 tcaccgccga cggtgcgccg cacctgccgg catccgatgc ggtgatcgac ggcgagatcg
     1321 tcgcgttcga cgcccagggc aggccgagct tcacccgcct gcagaaccgg atgcacctga
     1381 cccgcggccg cgagatcgag cgggaggttg tgcgcacgcc ggtcgtgtac ctgctgttcg
     1441 acctgatgcg cctcgacggc cacgacctca cccgcatgcc gctgcgacag cgacgcgaac
     1501 tgctggagca gctggcatcc gatctcgacg cgccggtgca ggtgccgccg gtgttcgacg
     1561 acctcgacgc ggcgatggag atgagcgggc gatacgggct ggagggcgtc gtcgccaagg
     1621 acccgggatc cccctaccgt cccggggcgc ggtcgtcgtc ctggctgaag ctcaagcaca
     1681 cccgcacgca ggaggtggtg gtggtcggca tccgccccgg tcagggcaac cggcgcgagg
     1741 gcatcggctc gctgctgctc gccgtgcccg acgacgacgg ggcgctgcgg tacgtcggcc
     1801 gggtcggcac cggcttcacc gatcgaatgc tgagcgatct gcgcgcacgc ctggagccgc
     1861 tgcgcacggg gagcccgg
//
LOCUS       DATMSX010000242         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_41681,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000242 DATMSX010000000
VERSION     DATMSX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41681"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..372)
                     /locus_tag="VN041_07375"
     CDS             complement(97..372)
                     /locus_tag="VN041_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATPase"
                     /protein_id="HWU28899.1"
                     /translation="MSTHDDSSGTSNGLLDRILAYTALALTAASIICFFAIPIGTATG
                     MQQKDFGQGAWPFIAALPLYGLPVAFLMIITLLIITFVRRGRANTRR"
     gene            499..867
                     /locus_tag="VN041_07380"
     CDS             499..867
                     /locus_tag="VN041_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWU28900.1"
                     /translation="MPTGKVRFYDDEKGFGFIASDDGQDVFLHASALPAGVSVKKNTR
                     VEFGLADGRRGPQALSVRVLDAPPARAKAPRANAHDMAVIIEDLMKMLDDASADLRHG
                     RPTSRALPAVLRKVADDLDA"
     gene            851..1462
                     /locus_tag="VN041_07385"
     CDS             851..1462
                     /locus_tag="VN041_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HWU28901.1"
                     /translation="MTSTPDELASATRELALAALHEITPASTVGPAAGHSVEESGAVS
                     LRFENRLPGYPGWYWTVSVAQVDGEEPTVLEVELMPGESALLAPDWVPWTERLAEYRA
                     HQAELAEQAAAAGEDADEDSDDADESDDEDSDGDDDHDESDLLHAGDLDGVDIDELDA
                     DADDQDDDSSDESDEDDESDDDDESDEDGSDEDEDGDDSDEEE"
     gene            complement(1525..2631)
                     /gene="serC"
                     /locus_tag="VN041_07390"
     CDS             complement(1525..2631)
                     /gene="serC"
                     /locus_tag="VN041_07390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWU28902.1"
                     /translation="MAIEIPRELLPVDGRFGCGPSKVRPAQVEALASVGESLLGTSHR
                     QLPVKNLVASARERLSTLFRLPDGYEIILGNGGSTAFWDAAAFGLIERRSQNLVFGEF
                     GGKFAKAAAAPWLEAPDVRNAEPGSLTSAEVVEGIDVYAWPHNETSTGVAAPVQRVAA
                     EGALTVIDATSAAGGIDFDVTQADVYYFAPQKNLGSDGGLWFAAVSPAAVERIERIAA
                     SGRYVPEFLSLKNALDNSRLSQTLNTPALTTLHLLDSQLAWILNHGGLEWAAARTAES
                     SGLLYDWAEATSVTTPFVVDPAHRSPVVATIDFDGVDAAAIAKTLRANGIVDTEPYRK
                     LGRNQLRIATFVSIEPDDVRQLIRSIEFVLEHQG"
     gene            2732..3433
                     /locus_tag="VN041_07395"
     CDS             2732..3433
                     /locus_tag="VN041_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWU28903.1"
                     /translation="MTDLIDTTEMYLRTILELEEENIVPLRARISERLGHSGPTVSQT
                     VGRMERDGLVVVSEDRRLELTDAGRRKAVDVMRKHRLAERLLSDVIGLDWAFVHEEAC
                     RWEHVMSEQVERRLVELLGHPTESPYGNPIPGLDQLGDVPTRSFDDGVIGLVKKLDAA
                     GEPIEGTVRRLAEPAQVDPELLEQLRDAGVVPGARGDYRYNEGYVLIRMEGREEGLEL
                     PVELASHIFLVGGNS"
     gene            3687..>3961
                     /locus_tag="VN041_07400"
     CDS             3687..>3961
                     /locus_tag="VN041_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HWU28904.1"
                     /translation="MAEDIATTDAAEDPKKSRKVRGRAASRTGTRSSVKPMRTVAIFA
                     AVGALVAAVALPAYAATGGASAKSGAATLQQMAADDAQSLVVASEATT"
BASE COUNT          653 a         1313 c         1402 g          593 t
ORIGIN      
        1 cgcaccgcac ggctgcgcag caggtcggcc agccgggcat cgctcaccgc ggccagcctg
       61 tcggccagcg ggcgcgcgtg ggtgctcatg ctcggctcag cggcgcgtgt tcgcgcgtcc
      121 cctgcggacg aacgtgatga tcagcagcgt gatgatcatc aggaacgcga cgggaaggcc
      181 gtagagcggc agcgccgcga tgaagggcca ggcgccctgg ccaaagtcct tctgctgcat
      241 gccggtggcg gtgccgatgg ggatcgcgaa gaagcagatg atggacgccg cggtcagcgc
      301 cagggcggtg tatgccagga tgcgatcgag cagaccgttt gaggtgccgc tgctgtcgtc
      361 gtgcgtgctc atcacactcc agcgtatcgc ctgggcggtt gcgcgggatc gagcgtccgc
      421 accgggtgca cgggtaggct ggaggggacg cgccgccgcg cgacgattca cgacagcgac
      481 attcagcgag gttctcccat gcccaccggc aaggtcaggt tctacgacga cgagaagggg
      541 ttcggcttca tcgcatccga tgacggccag gacgtcttcc tgcacgcctc tgcccttcct
      601 gcaggcgtct ccgtgaagaa gaacacccgc gtggagttcg gcctcgcaga cggccgccgc
      661 ggcccgcagg ccctctccgt gcgcgtgctg gatgcaccgc ctgcccgcgc gaaggccccg
      721 cgcgcgaacg cccatgacat ggccgtgatc atcgaggacc tcatgaagat gctcgatgac
      781 gcgagcgcgg acctgcgcca cggtcgtccc accagccggg ctctgcccgc cgtgctccgc
      841 aaggtcgctg atgacctcga cgcctgacga actcgcttcg gcgacgcggg agctcgcgct
      901 cgccgcgctg cacgagatca ccccggcatc caccgtcggc ccggcggccg gtcactccgt
      961 cgaggagagc ggcgcggtct cgctgcgctt cgagaaccgc cttcccggat acccgggctg
     1021 gtactggacg gtctcggtcg ctcaggtcga cggcgaggag ccgacggtgc tcgaggtcga
     1081 gctgatgccc ggcgagagcg ccctcctggc gccggactgg gtgccgtgga ccgagcggct
     1141 ggcagagtac cgcgcgcacc aggcggagct cgccgagcag gcggccgccg ccggtgagga
     1201 cgcggacgag gactccgacg acgccgacga gtccgatgac gaggactccg atggggacga
     1261 cgaccacgac gagagcgacc tgctgcacgc cggtgacctc gacggtgtcg acatcgacga
     1321 gctggacgcg gacgccgacg accaggacga cgactcatcc gacgagtccg atgaggatga
     1381 cgagtccgat gacgacgacg agtccgacga ggacggctca gacgaggatg aggacggtga
     1441 cgactccgac gaggaggagt gaccccagaa cggagaacgc ccgcacgacc tgacggtctg
     1501 tgcgggcgtt ccgtgctttg gggctcagcc ctggtgctcg agcacgaact cgatcgagcg
     1561 gatcagctgg cgcacgtcat cgggttcgat ggacacgaac gtcgcgatgc gcagctggtt
     1621 gcggccgagc ttgcggtagg gctcggtgtc gacgatgccg ttggcgcgca gcgtcttggc
     1681 gatggctgcc gcatcgaccc cgtcgaagtc gatggtcgcg acgacggggg agcggtgagc
     1741 cgggtcgacg acgaacggcg tggtgaccga cgttgcctcg gcccagtcgt acaggagacc
     1801 ggacgactcg gcggtgcggg cggctgccca ctccagtccg ccgtggttca ggatccacgc
     1861 gagctggctg tccagcagat gcagcgtggt cagcgcggga gtgttcagcg tctggctgag
     1921 ccgcgagttg tccagggcgt tcttcaggct gaggaactcc gggacgtacc ggccggatgc
     1981 cgcgatgcgc tcgatgcgct cgacggcggc gggggagacg gccgcgaacc acaggccgcc
     2041 gtcggagccg agattcttct gcggcgcgaa gtagtagacg tctgcctgcg tcacatcgaa
     2101 gtcgatgccg ccggccgcgc tggtcgcgtc gatcacggtg agggcgccct ctgcggcgac
     2161 gcgctgcacg ggggcggcca cgccggtgga ggtctcgttg tgcggccagg cgtagacatc
     2221 gatgccctcg acgacctcgg ccgaggtcag cgatccgggc tccgcgttgc gcacatccgg
     2281 ggcctccagc cacggggccg cggccgcctt ggcgaacttg ccgccgaact cgccgaacac
     2341 caggttctgg ctgcggcgct cgatcagtcc gaaggccgcg gcgtcccaga acgcggtgga
     2401 tccgccgttg ccgaggatga tctcgtatcc gtcgggaaga cggaacaggg tggagaggcg
     2461 ctcacgcgcg ctcgcgacca ggttcttgac gggcagctgg cgatgggacg tgcccagcag
     2521 actctctccg accgaggcga gcgcctcgac ctgcgcgggg cgcactttgg acggaccgca
     2581 gccgaagcgt ccgtcgacgg gcaggagttc tcgggggatc tcgatcgcca tgggtcgatt
     2641 gtaggcgggt atcccgtgcc catccgcccg catgacggcg acgagctcct gtaaaaaggt
     2701 aggcttgcct aagaaccttc ggagggcccg catgaccgat ctgatcgaca cgaccgagat
     2761 gtatctccgc accatcctcg agctcgagga ggagaacatc gtgcctctgc gcgcgcgcat
     2821 ctccgagcgc ctcgggcact ccggtccgac cgtgtcgcag accgtggggc gcatggagcg
     2881 cgacggcctc gtcgtcgtct ccgaggaccg ccggctcgag ctcacggatg ccggccgccg
     2941 caaggccgtc gacgtcatgc gcaagcatcg cctcgccgag cggctgctct ccgacgtgat
     3001 cgggctggac tgggcattcg tgcacgaaga ggcctgccgg tgggagcacg tcatgagcga
     3061 gcaggtcgag cgtcgcctcg tggaactgct cggtcatccg acggagtcgc cgtacggcaa
     3121 cccgattccc ggcctcgacc agctcggcga cgtcccgacg cgatccttcg acgacggcgt
     3181 gatcggactg gtcaagaagc tggacgccgc cggagagccg atcgagggca ccgtccgacg
     3241 cctcgccgag cccgctcagg tggatcccga actgctcgag cagctgcgtg acgccggtgt
     3301 cgtccccggc gctcgagggg actaccggta caacgagggc tacgtcctca tccgcatgga
     3361 aggccgcgaa gagggtctgg agctgccggt cgagctggcg tctcacatct tcctggtcgg
     3421 cgggaactcc tgatcagatg atctttcgcc ctccgccagg aagattgcca ggttcggagg
     3481 gtgacagaat cgttatcttc cggtaacgtc gttggggtcg ccgacgaaga agcccgtcgg
     3541 tgacgatcac ggggatcgcc tccctggctc gtcgtccccg tgagcagttc gcacaacgtg
     3601 ccgtgtgaca tacgtgcccg agaatcgaca ttgctgacga gcctgcgcta gcccgaatga
     3661 gcgcggaggc gcaggaggaa tcccttttgg ccgaagacat cgccacgacc gacgcagctg
     3721 aagacccgaa gaagagcagg aaggtgcgag gacgcgccgc cagccgcaca ggcacccgct
     3781 ccagcgtgaa gcccatgcgc actgtcgcga tcttcgcggc agtcggtgct ctcgtcgccg
     3841 ccgtcgccct tcccgcctac gcagcgaccg gcggcgcgtc cgccaagtcc ggtgccgcga
     3901 cccttcagca gatggccgcc gacgacgcgc agtcgctcgt cgtggcatcc gaggccacca
     3961 c
//
LOCUS       DATMSX010000243         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_60580,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000243 DATMSX010000000
VERSION     DATMSX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60580"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..501
                     /locus_tag="VN041_07405"
     CDS             151..501
                     /locus_tag="VN041_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU28905.1"
                     /translation="MPESSDLDLVLALERELQTPACRSDPARLEELLAPEYIEFGASG
                     QVWSRTEIIAELAGEDGGVIEVHDLEAREVSDDVVLVLWRSRMADRQALRSSLWHREP
                     SGWRLVHHQGTPTG"
     gene            complement(585..1016)
                     /locus_tag="VN041_07410"
     CDS             complement(585..1016)
                     /locus_tag="VN041_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU28906.1"
                     /translation="MNDVILVDLESEDPRWARALPVLRELRPHLTEELFTQVMREAGP
                     QGLRFTAVFAGERCVAIAGWRIVANTSAIRKLYIDDLCTAAADRSHGYGRMLMDELRE
                     RAVAAGCRSLELDSGVQRFDAHRFYLRERMSITAHHFDLEL"
     gene            complement(1013..1528)
                     /locus_tag="VN041_07415"
     CDS             complement(1013..1528)
                     /locus_tag="VN041_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU28907.1"
                     /translation="MPDRLIVLNGMAGAGKTTLARPLAAELGVPFVSKDALKEALGDA
                     VDAPLPTRALGALAADALWRITGMLDGTVLVESVWLADRDEEWFRRGWESTGSPAGVE
                     VWCEAPREVMRERFFTRPRHSAHDDQGRRDEWEQFADAARPMTGFPVVTVDTTGPVDV
                     TALARAVKEML"
     gene            complement(1521..>2389)
                     /locus_tag="VN041_07420"
     CDS             complement(1521..>2389)
                     /locus_tag="VN041_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWU28908.1"
                     /translation="RVEHIGSRGALDVEALGEVTAAALTQPSHPAVPPLDTEAGLFSL
                     SLEQIVPISVVVRDSETGLPKEDDDGIVKTRSPFSRNPTADEKKAGIEGPQPSSAAVK
                     LLAELEKAKTKELWRLLVSLNIRHVGPVAARALAQWFGSLDAIRAASRDELAAVEGVG
                     GIIADSLLNWFEVDWHQDIVQQWADAGVQWSTPGHPGPGAAVAEGGVLDGLTVVATGS
                     LDGYTREGAQEAIIKAGGKAASSVSKKTDFVAAGPGAGSKLAKAEELGIRVLDAAQFH
                     VLVTQGPDALDA"
BASE COUNT          345 a          891 c          807 g          346 t
ORIGIN      
        1 gggatcgccg tggcggatac caggacgacc ggcgcgggcg cgacgagggg cgcgaccggg
       61 gctactcccg ggatgaccgc cccgctcgcg gcgatcgctt cgagcgcgat gaccgtccgg
      121 cccggaagcc ccgtcacaag ggctgaccgg atgccggaga gcagcgacct cgatctggtg
      181 ctcgcgctgg agcgcgagct ccagacgccg gcctgtcgtt ccgaccccgc ccgactcgag
      241 gagctgctcg ccccggagta catcgagttc ggagcgtcgg gtcaggtctg gagccgcacc
      301 gagatcatcg ccgagctcgc cggcgaggac gggggcgtca tcgaagtcca cgacctcgaa
      361 gcccgcgagg tctccgacga cgtggtgctc gtgctctggc gatcccgcat ggcggaccgg
      421 caggcgctgc gcagctcgct ctggcatcgc gagcccagcg gctggcgtct ggtgcaccat
      481 cagggcacac ccaccggctg agcccgccat cggctaagcg cgcccgcgcg tcgagttcgc
      541 agggctccgt cgaccgcctt actcgttcgc gccaggaccg ccgctcagag ctcgagatcg
      601 aagtggtgcg cggtgatgct catccgctcg cgcaggtaga accggtgcgc gtcgaagcgc
      661 tgcaccccgg agtccagttc cagggatcgg cagccggcgg cgacggctcg ctcgcgcagc
      721 tcgtccatca gcatccgccc gtacccgtgc gaccggtcgg cggcggccgt gcacaggtcg
      781 tcgatgtaca gcttgcggat ggcgctggtg ttggccacga tccgccaccc tgcgatcgcc
      841 acgcacctct cgccggcgaa gaccgccgtg aaccggagcc cctgcggtcc cgcctcccgc
      901 atcacctggg tgaacagctc ttccgtcagg tgcgggcgca gctcccgcag caccggcagg
      961 gcgcgtgccc agcgcgggtc ctcggactcc aggtccacca ggatcacgtc gttcacagca
     1021 tctccttcac agcgcgggcg agggccgtca cgtcgacggg accggtggtg tcgactgtca
     1081 ccacggggaa gccggtcatc ggccgcgctg catccgcgaa ctgctcccac tcgtcgcgtc
     1141 tgccctggtc gtcgtgcgcg gagtgccgtg gccgggtgaa gaagcgctcg cgcatgacct
     1201 cgcgtggcgc ttcgcaccac acctccacgc ccgccggcga gcctgtggac tcccagccgc
     1261 gccggaacca ctcctcgtcg cggtcggcca gccagaccga ctcgacgagc accgtcccat
     1321 ccagcatccc ggtgatccgc cagagcgcat ccgcggcgag agcgccgagg gcgcgggtgg
     1381 gaagcggcgc gtccacagcg tcgccgagag cttccttgag ggcgtccttc gacacgaacg
     1441 gcacgccgag ctcggcggcc agcgggcgag cgagcgtcgt cttccccgcc ccggccatcc
     1501 cgttcaggac gatgagacgg tcaggcatcc agcgcgtccg ggccctgcgt caccagcacg
     1561 tggaactgcg cggcgtccag cacccggatg ccgagttcct cggccttcgc gagcttcgac
     1621 cccgctccgg gacctgcagc gacgaaatcg gtcttcttgg agacggacga cgccgctttg
     1681 ccaccggcct tgatgatcgc ctcctgcgcg ccctcgcgcg tgtagccgtc cagggaaccg
     1741 gtggcgacga cggtgagacc gtcgagcacc ccgccctcgg cgaccgccgc gcccgggccc
     1801 gggtgaccgg gcgtggacca ctgcacaccg gcatccgccc actgctgcac gatgtcctga
     1861 tgccagtcga cctcgaacca gttcagcagc gagtctgcga tgatgccgcc cacgccctcc
     1921 accgctgcca gctcgtcccg cgatgcggcg cggatcgcgt cgagggagcc gaaccactgc
     1981 gcgagagcgc gtgcggcgac cgggccgaca tggcggatgt tcagcgagac cagcagccgc
     2041 cacagctcct tggtctttgc tttctccagc tcggcgagca gcttcacagc ggcggacgac
     2101 ggctgcggtc cctcaatgcc cgccttcttc tcgtcggcgg tggggttccg gctgaacggc
     2161 gatcgggtct tcacgatccc gtcgtcgtcc tccttcggca gacccgtctc ggagtcgcgc
     2221 accacgaccg agatcggcac gatctgctcc agggagagcg agaacagtcc ggcctcggtg
     2281 tccagcggcg gcactgcggg atggctcggc tgcgtgagcg ctgcggccgt gacctcaccg
     2341 agcgcctcca cgtccagcgc gccgcgggac ccgatgtgct ccacgcgcc
//
LOCUS       DATMSX010000244        10865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_155358,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000244 DATMSX010000000
VERSION     DATMSX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155358"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VN041_07425"
     CDS             <1..154
                     /locus_tag="VN041_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HWU28909.1"
                     /translation="CAQFADAPEHESGARAVTAYAGAGIVAGSDPEAELLETRVKFRP
                     LVDALA"
     gene            241..1302
                     /locus_tag="VN041_07430"
     CDS             241..1302
                     /locus_tag="VN041_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWU28910.1"
                     /translation="MTSSPASPGTRLASRLGFSDRVFLGPAARRIATRIEEGLDMVEA
                     GLADELRMADPVADAVARYLYEAGGKRIRPVLTLLTAQLGDGNSAAVIDIAKALELTH
                     LGSLYHDDVMDGADTRRGVPAAHGVWGNNVAILTGDILFSRASQIMSRHGERAMRLQA
                     DTFERLVLGQMHETVGAQPGDDPIEFYIQVLADKTGSLIAAATQGGVIFSGAPAEYEE
                     PLRVYGEKVGVAFQLLDDVIDLSADAEETGKVPGTDLRAGVPTMPSLLLKTGTAPADI
                     DLAARIDEGVARIAEGADPAVLDGPLAELRDHDATRRTMELARTWTADAVAALAPLPK
                     GTVRESLTRFAESLADRSS"
     gene            1317..2696
                     /locus_tag="VN041_07435"
     CDS             1317..2696
                     /locus_tag="VN041_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU28911.1"
                     /translation="MTKLKLAIVGAGPAGIYAADILLKAERKFDVSIDLFEQLPAPYG
                     LVRYGVAPDHPRIKGIINALRNVIDHGDMRLFGNVHFGTDITLEDLKRHYNAVIFATG
                     AVRDSALDIPGIDATHSFGAAEYVSWFDGHPDVSPEWTLDAESVGVIGNGNVALDVAR
                     MLAKHADDLLVTEIPDNVYQGLKASRITDVHVFGRRGPAQVKFTPLELRELGELRDVD
                     MVVYDEDFQYDEASQQAVASNKQVMVMDRIMQSWRKLDSVNNAGGTASRRLHLHFWAR
                     PVEVKKDENGRVAALVYERTESDGAGGAVGTGELREVPMQGLYRAIGYFGSPLAGVPF
                     DKKHGVIPNREGQVLETKSNQRVPGIYTTGWIKRGPVGLIGHTKSDAMETVQHLINDQ
                     GSWWHPEDPSDEAIPALLAERGVQWTDLDGWHRLDAHEIALGEAHESTRERVKLVPRD
                     EMVRVSRGE"
     gene            2756..3742
                     /locus_tag="VN041_07440"
     CDS             2756..3742
                     /locus_tag="VN041_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU28912.1"
                     /translation="MGEWVPDVLGDEFQQLTLPLEPDAEGDVVATVVRALPEPRPWWH
                     VRDRRPLADVDVLYVHGWSDYFFQKRLARFWTSRGARFFALDLRKYGRSLRPGQTPGF
                     ITDLAEYDADIEAALAARGDAEGRRLVLLGHSTGGLTLSLWASRHPRAVDAVILNSPW
                     LEFQLGAARTAIAPMVELQARWWPKDAAPQVDLGFYTRAQNEVADPDDPMEVDLAWRP
                     EQTMTVHAAWLHAILQGHRAVSAGLHIDAPVCVLLSARTAVPTRWSDELTRADSVLVV
                     DDIARAALKLGPSVTVERIDGALHDVFLSRREAREDAYARLERWVTGWVASR"
     gene            complement(3761..4384)
                     /locus_tag="VN041_07445"
     CDS             complement(3761..4384)
                     /locus_tag="VN041_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU28913.1"
                     /translation="MGSASTAKKQLLAEGVFHLIGERIVTGELAPGDRIRDADVAQEL
                     AVSRTPVREALQRLERIGLVTMYPSRYTEISRLTPETIRMSHDFAGLQAGVIVRLSCP
                     RLTTADHETLSPMIARIAASVDDPAACSQARREVIGYLAARSGNTLQQSLVDEASMAL
                     ARALRGFAITPEHKPRLLDACVELDRALRDGDADAAEQACRVIYDVA"
     gene            complement(4557..5045)
                     /locus_tag="VN041_07450"
     CDS             complement(4557..5045)
                     /locus_tag="VN041_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWU28914.1"
                     /translation="MADSSFDIVSKVDHQEADNALNQARKEIEQRYDFKGTGASISWS
                     GEQIVIVANSEERAKAVLDVFQTKLIKRGISLKSLESGDPVASGKEYRLTSTLKDGIS
                     SENAKKIGKIIRDEGPKGVKSQIQGDELRVQSKSRDDLQAVIALLKGSDLDIDLQFIN
                     YR"
     gene            5236..6471
                     /locus_tag="VN041_07455"
     CDS             5236..6471
                     /locus_tag="VN041_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU28915.1"
                     /translation="MKVTKRSIAAFGAITVLTSLALAGCSAANTGDKGTDGDSAKGGK
                     LVVWVDSERVDAVKGAAEAYEKKTGVKVQLVGKNVDDIKDDFIQQVPTGKGPDITMGA
                     HDWLGELSTNGVVAPLELGDSSKDYLPVALQASTYDGNVYMLPYAVENIAVLRNTAIV
                     PEAATSFDDMIAKGKAAGLDQPFVVEQGAEGNPYHLYPFQTAFGAPVFGTDAKGGYDP
                     TNLQLGSEGGTAFANWLAAQGKAGTLNTDIDGEIAKQQFLDGKAAFWLTGPWNVGAAT
                     DAGIKVAIDKIPSPTDKAASPFAGVKGFFISAESKNKVAANDFLVNYIGTPDVQLDLF
                     KAGNVLPALTAAADKAASDPIIAGFQAVGADAVPMPAIPAMGSVWQFWGVAEAAIIKG
                     ADPQATWTKLADDVAGAIK"
     gene            6579..8195
                     /locus_tag="VN041_07460"
     CDS             6579..8195
                     /locus_tag="VN041_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU28916.1"
                     /translation="MSQATELEAPQAPRTRKQRQAAAIAEAASGHVGWLLLKIGLLAI
                     VDAIALYAAFVLYTASEWLVLGVVVIVAILVNWIYFSRRRVPAKYLTPGVIFLILFQV
                     FTLLYTGYIGFTNYGTGHNGSKDQAVAALMRSAQERVADSPTYGVTVVEKLGTLGLLV
                     TDPEDGDVWVGTDEQPLQKVQGAGMDGDTAVSVDGWTSLSFADVISRSDDVEKLSVPF
                     SEDPNDGAIRTPDGRRGYLYVSTLEYDAPAGTMTDTTSGTVYRDTGVGAFTAEGGEQL
                     LPGWQITVGFDNFLRAVTDASIRGPLISVTIWTFVFALISVASTFFLGLLLALVFNNT
                     RMRFRNGYRILLILPYAFPAFLSALVWAGMMNESFGFLNQVVFGGASIPWLTDPTLAK
                     VSVLIVNLWLGFPYMFLVCMGALQGIPSDVNEAASMDGANPWQIFRSIKLPLLLVTVA
                     PLLISSFAFNFNNFNLIYMLTNGGPRFDDVSIPVGHTDILISMVYKVAFTGQTRDYGL
                     ASAFTILIFIVVAAISIISFRKTKALEELN"
     gene            8197..9093
                     /locus_tag="VN041_07465"
     CDS             8197..9093
                     /locus_tag="VN041_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU28917.1"
                     /translation="MSTASSTRDLRPARRSFGKWFGDTGWRHVVAIIVSAFAVFPLLY
                     VVSASLNPKGTLTGSNRLFSAVGIDSYVRILTDPKNPYPMWFWNTLVIATVTGFVTVF
                     LGALAAYAFSRMRFTGRRVGLVTIVVVQMFPQLLAVVAIFLLMSTLGDWFPAIGLNTH
                     TGLILVYLGGALGVNTYLMYGFFNTLPMELDEAARIDGAGHARIFFTMILPLVAPILA
                     VVALLSFIGTVNEYVIASIMLIDPDKQTLVVGLTKLVANPRYADWSAFSAGAVMAAVP
                     VMVLFLLLQKYIVGGLTAGAVK"
     gene            9109..>10865
                     /locus_tag="VN041_07470"
     CDS             9109..>10865
                     /locus_tag="VN041_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HWU28918.1"
                     /translation="MTLPHHDGSPLFVSDDAPALGDTVAVRLRVPAGYGPLRAVRTRS
                     NPDHEPEWTDAVLLGAADGWDWWEAPIHVRNPRHGYRFVLLHEDGRVEWLNQTGIHTG
                     ETLDAEDFALAATPAPPAWLHGSVMYQVFPDRFARSAQADAHPTPDWAITASWDDPVD
                     PVVPGRSQQFYGGDLPGIVEHLDHLVALGVDLLYLTPVFPAGSNHRYDASSFDTIDPL
                     LGGEDAYVKLIEEAHARGIRVIGDLTSNHSGDRHEWFQQALGHPGAETESFYYFTDAD
                     NTTYESWLGTPTLPKFNWSSDALRERFITAEDSVVAKWLKPPYSIDGWRIDVANMTGR
                     LGAVDLNAEVRQLLADTVRAINPDAIVLGESTNDAATDLQGDGWHGAMTYPAFTRPLW
                     GWLSEPTGAPYLTYDGERATEPWFFTQPIGGIPRYTARDFADAVTRFTAGIPWRVRLG
                     NMQPLDTHDTARFATNAAPGALPVAVGLSMTMPGVPVVFAGDEFGLTGADGESSRTPM
                     PWSRQDEPELAEVFETYRSLVGLRRAHPVLGTGGMRWLHVDDETVVFVRESAEESVLV
                     LATRGGADIELAPGALPGAA"
BASE COUNT         1679 a         3803 c         3668 g         1715 t
ORIGIN      
        1 ctgcgcccag ttcgccgacg ctcccgagca cgagtccggc gctcgcgccg tgacggccta
       61 cgccggcgcc ggcatcgtgg cggggagcga ccccgaggcg gagctgctgg agacccgtgt
      121 gaagttccgt ccgctcgtgg acgccctggc ctgagtgatc gcggggccgg agaagccgtc
      181 tgcgactcgt cagtcaccgg acggacatgc gcgcccgtgc gcgcaggtag gctggaacac
      241 gtgacttcga gccccgcttc cccgggcacg cgactggcga gccgactcgg cttcagcgac
      301 cgcgtcttcc tcggtcccgc cgcgcgacgc atcgccacgc gcatcgagga gggcctcgac
      361 atggtcgagg cggggctggc cgacgagctg aggatggcgg accccgtcgc ggacgccgtg
      421 gcgcgctacc tctacgaggc cggtggcaag cgcatccgcc ccgtgctcac gctgctgacg
      481 gcccagctcg gcgacggcaa cagcgcggcc gtgatcgaca tcgccaaggc gctggagctc
      541 acccacttgg gctcgctgta ccacgacgac gtcatggacg gcgcggacac ccgacgcggc
      601 gtcccggccg cgcacggcgt gtggggcaac aacgtcgcga tcctcaccgg tgacatcctc
      661 ttctcgcgcg ccagccagat catgtcgcgg cacggcgagc gggcgatgcg cctgcaggcg
      721 gacaccttcg agcgcctcgt gctcggtcag atgcacgaga ccgtgggtgc gcagccgggc
      781 gacgacccca tcgagttcta catccaggtg ctcgccgaca agaccggctc gctgatcgcc
      841 gcggccacgc agggcggcgt catcttctcc ggcgcccccg ccgagtacga ggagccgctg
      901 cgcgtctacg gcgagaaggt cggcgtcgca ttccagctgc tcgatgacgt gatcgacctc
      961 tctgcggatg ccgaggagac cggcaaggtt cccggcaccg acctgcgggc gggcgtcccc
     1021 acgatgcctt cgctgctgct gaagaccggc acggcgcccg ccgacatcga cctggccgcg
     1081 cgcatcgacg agggcgtcgc gcgcatcgcc gagggggcgg atcccgccgt cctcgacggg
     1141 ccgctcgcag aactgcgcga tcatgacgcc acccggcgca ccatggagct cgcccgcacc
     1201 tggacggccg atgccgtcgc cgcgctcgca ccgctgccca agggcaccgt gcgcgagtcg
     1261 ctgacccggt tcgcggagtc tctggcagac cgcagcagct gacggaagga accccattga
     1321 cgaagctcaa gctggccatc gtcggggcgg gaccggcggg catctacgcc gctgacatcc
     1381 tgctcaaggc ggagcgcaag ttcgatgtct ccatcgacct gttcgagcag cttccggcgc
     1441 cgtacggcct ggtccgctac ggcgtcgcgc cggatcaccc gcgcatcaag ggcatcatca
     1501 acgcgctgcg gaacgtgatc gaccacggcg acatgcgcct gttcggcaac gtccacttcg
     1561 gcaccgacat cacgctcgag gatctcaagc gccattacaa cgcggtgatc ttcgcgaccg
     1621 gcgcggtccg cgactcggcc ctggacatcc ccggcatcga cgcgacccac tccttcggcg
     1681 cggccgagta cgtgagctgg ttcgacgggc atccggacgt ctcgccggag tggacactgg
     1741 atgccgagtc ggtcggcgtc atcggcaacg gcaacgtcgc gctggacgtg gcgcgcatgc
     1801 tcgccaagca cgccgacgac ctgctcgtga ccgagatccc cgacaacgtc taccaggggc
     1861 tgaaggccag ccggatcacc gatgtgcacg tgttcggccg gcgcggtccg gcgcaggtga
     1921 agttcacccc gctcgagctg cgcgagctcg gcgagctgcg cgacgtcgac atggtcgtgt
     1981 acgacgagga cttccagtac gacgaggcct cccagcaggc cgtcgccagc aacaagcagg
     2041 tcatggtcat ggaccggatc atgcagtcct ggcgcaagct cgactccgtg aacaacgcgg
     2101 ggggcacggc atcccgccgt ctgcacctgc acttctgggc gcgcccggtc gaggtgaaga
     2161 aggacgagaa cggccgcgtc gcggccctcg tctacgagcg caccgagtcc gacggcgccg
     2221 gcggcgcggt cggcacgggc gagctccgcg aggtccccat gcaggggctg taccgcgcga
     2281 tcggctactt cggctcgccg ctggccggtg tgccgttcga caagaagcac ggcgtcatcc
     2341 cgaaccgcga gggccaggtg ctggagacca agtcgaacca gcgggtgccc ggcatctaca
     2401 cgaccggctg gatcaagcgc ggtccggtgg gcctgatcgg ccacaccaag tcggatgcca
     2461 tggagaccgt gcagcacctg atcaacgacc agggctcgtg gtggcacccg gaggacccct
     2521 ccgacgaggc gatcccggcg ctgctcgccg agcgcggcgt ccagtggacc gacctggacg
     2581 gctggcaccg cctggacgcg cacgagatcg ccctgggcga ggcccacgag agcacgcgcg
     2641 agcgcgtcaa gctcgtgccg cgcgacgaga tggtccgcgt ctctcgcggg gagtgagcgg
     2701 tcccctcgga gccgtgggct cgaagcgccg tccggccgac cgataggttg acctcatggg
     2761 cgagtgggtt ccggatgtcc tcggcgacga gttccagcag ctgacgctgc cgctggaacc
     2821 ggatgctgag ggcgacgtgg tcgcgacggt cgtgcgcgcc ctgcccgagc cgcggccgtg
     2881 gtggcacgtc cgcgaccggc gtccgctcgc ggatgtcgac gtgctgtacg tgcacgggtg
     2941 gtcggactac ttcttccaga agcgcctggc ccgcttctgg acctcgcgcg gtgcgcggtt
     3001 cttcgctctc gacctgcgca agtacgggcg cagcctgcgt cccgggcaga cgccgggctt
     3061 catcacggac ctcgccgagt acgacgccga catcgaggcc gcgctcgctg cgcgagggga
     3121 tgccgagggg cgtcggctcg tgctgctcgg gcactccacg ggcgggctga cgctcagcct
     3181 gtgggcgtca cggcatccgc gcgcggtcga tgccgtcatc ctcaactcgc cgtggctcga
     3241 gttccagctc ggcgctgcgc gcacggcgat cgcgccgatg gtcgagctgc aggcgagatg
     3301 gtggccgaag gacgccgcgc cgcaggtgga tctgggcttc tacacgcgtg cgcagaacga
     3361 ggtggccgac cccgacgatc ccatggaggt ggatctggcc tggcgtcccg agcagaccat
     3421 gaccgtgcac gccgcgtggc tgcacgcgat cctgcagggc catcgggcag tctccgcagg
     3481 tctgcacatc gacgcgccgg tctgcgtgct gctgtcggcg cgcaccgcgg tgccgacgcg
     3541 ctggtcggac gagctcacga gggccgacag cgtgctggtc gtcgacgaca tcgcccgtgc
     3601 cgccctgaag ctcgggccgt cggtgacggt cgagcgcatc gacggcgcat tgcacgacgt
     3661 cttcctgtcg cgacgcgaag cgcgcgagga cgcttacgcc cgcctggagc gctgggtcac
     3721 cgggtgggtg gcctcgcgct gacggggcgg atgcccctga tcacgccacg tcgtagatga
     3781 cgcggcaggc ctgctctgcg gcatccgcat ctccgtcgcg cagcgcccgg tccagctcga
     3841 cgcaggcgtc gagaaggcgc ggcttgtgct cgggggtgat cgcgaacccg cgcagagcgc
     3901 gagccagcgc catggaggcc tcgtcgacca gcgactgctg cagcgtgttg ccgctccggg
     3961 cggcgaggta gccgatcacc tcgcgtcgag cctgcgagca ggcggcgggg tcgtcgacgc
     4021 tcgccgcgat ccgcgcgatc atcggcgaca gcgtctcatg gtcggcggtc gtcagccgcg
     4081 ggcaggacag ccgcacgatg acgccggcct gcaggccggc gaagtcgtgc gacatccgga
     4141 tggtctcggg cgtgaggcga ctgatctcgg tgtaccggct ggggtacatc gtcaccaggc
     4201 cgatgcgctc gagccgctgc agcgcctctc ggacgggcgt gcgcgacacc gccagctcct
     4261 gcgccacatc ggcgtcgcgg atgcggtcgc cgggcgcgag ctcgcccgtg acgatgcgtt
     4321 cgccgatcag gtggaacacc ccttcggcca gcagctgctt tttcgccgtg ctcgcggacc
     4381 ccatccgctg ttcttcgtcg acggacaccc cgatgacccc cagattcccc aatgacccag
     4441 ggattcccca cgaatccccg ggcggagcgt cccctaacgc cccgctgggg cccactctag
     4501 cgggtgcgcg ggcatctgag gagagggcag gatgccggta tgacggcggc tgcgggtcag
     4561 cggtagttga tgaactgcag gtcgatgtcg aggtcgctgc ccttcaggag cgcgatcacg
     4621 gcctgcaggt cgtcgcggct cttcgactgc acgcgcagct cgtcgccctg gatctgggac
     4681 ttcacgccct tcgggccctc gtcgcggatg atcttgccga tcttcttcgc gttctcgctg
     4741 gagatgccgt ccttgagggt cgacgtcagg cggtactcct tgccgctggc gacggggtcg
     4801 ccggactcca ggctcttcag cgagatgccg cgcttgatga gcttcgtctg gaacacgtcc
     4861 agcaccgcct tcgcgcgctc ctcggagttc gcgacgatga cgatctgctc gccgctccac
     4921 gagatggagg cgccggtgcc cttgaagtcg tagcgctgct cgatctcctt gcgggcctgg
     4981 ttgagggcgt tgtccgcctc ctggtgatcc accttggaca cgatgtcgaa tgagctgtca
     5041 gccatgcccg aagtctatcg aggatgccgt ttcatcgtct cgttggaagc gctttcaagc
     5101 tgttgcagtg cctgacttcc cgaataacag gttcatatcg accactcctt ccattgcgtt
     5161 accgtgcccg agcgatgcat actgtaagcg cttgcaatca tgcaggcccc actacatcga
     5221 gagaggcacc caccaatgaa ggtgaccaag aggagcatcg cggcgttcgg cgcgatcact
     5281 gtcctgacct cgctggcgct cgcgggctgc tcggccgcga acacgggcga caagggaaca
     5341 gacggcgact ccgccaaggg cggcaagctg gtcgtctggg tcgactccga gcgagtcgac
     5401 gcggtcaagg gcgcggctga ggcgtacgag aagaagacgg gcgtgaaggt ccagctcgtc
     5461 ggcaagaacg tcgacgacat caaggacgac ttcatccagc aggtgccgac cggcaagggc
     5521 cccgacatca ccatgggcgc gcacgactgg ctcggcgagc tgtccaccaa cggcgtcgtg
     5581 gctccgctcg agctgggcga ctcgtccaag gactacctgc ccgtcgcact gcaggcctcg
     5641 acctacgacg gcaacgtcta catgcttccc tacgccgtcg agaacatcgc cgtgctgcgc
     5701 aacaccgcca tcgtgcccga ggccgcaacc agcttcgacg acatgatcgc gaagggcaag
     5761 gcggccggtc tcgaccagcc gttcgtggtc gagcagggcg ccgagggcaa cccctaccac
     5821 ctgtacccgt tccagaccgc gttcggtgct cccgtcttcg gcaccgatgc gaagggcggc
     5881 tacgacccga cgaacctcca gctgggcagc gagggcggca ccgcgttcgc caactggctg
     5941 gcagcgcagg gcaaggccgg cacgctgaac accgacatcg acggtgagat cgccaagcag
     6001 cagttcctcg acggcaaggc ggccttctgg ctgaccgggc cctggaatgt cggcgccgcc
     6061 accgacgcgg gcatcaaggt cgccatcgac aagatcccca gcccgaccga caaggccgcc
     6121 tcgccgttcg ccggcgtgaa gggcttcttc atcagcgccg agtccaagaa caaggtcgcc
     6181 gcgaacgact tcctcgtgaa ctacatcggc acgcccgacg tccagctcga cctgttcaag
     6241 gccgggaacg tgctgcccgc cctcaccgcg gcggcggaca aggcggcatc cgacccgatc
     6301 atcgccggtt tccaggccgt gggcgccgac gccgtgccga tgcccgccat ccccgccatg
     6361 ggctcggtct ggcagttctg gggcgtcgcc gaggccgcga tcatcaaggg cgcggacccg
     6421 caggccacct ggaccaagct ggccgacgac gtcgcaggcg cgatcaagta atcgcgacag
     6481 ccgaccgcgg ccgaccggcc gaggtcgagt gaagcagtcc accgctgccg ggacggggca
     6541 cgtgccccgt cccggcagcc gtgcacaaag gggtgaggat gagccaggcc actgagctgg
     6601 aggcgccgca ggcgccccgc acgagaaagc agcgccaggc tgccgcgatc gccgaagcgg
     6661 cgtcggggca tgtcggctgg ctgctgctga agatcggcct tctggcgatc gtcgacgcga
     6721 tcgccctgta cgcggcattc gtgctctaca cggcatccga atggctcgtg ctgggcgtcg
     6781 tcgtcatcgt ggcgatcctg gtcaactgga tctacttctc gcggcgacgt gtgccggcga
     6841 agtacctgac gcccggcgtg atcttcctga tcctgttcca ggtgttcacg ctgctgtaca
     6901 ccgggtacat cggcttcacc aactacggca ccgggcacaa cggcagcaag gaccaggcgg
     6961 tcgcagcgct catgcgctcc gcccaagagc gcgtggcgga ctcgcccacc tacggcgtga
     7021 ccgtcgtcga gaagctcggc acactgggcc tgctggtcac cgaccccgag gacggcgacg
     7081 tctgggtcgg caccgacgag cagccgctgc agaaggtgca gggtgccggc atggacggcg
     7141 acacggccgt gtccgtcgac gggtggacga gcctgtcgtt cgccgacgtg atctcccgct
     7201 ccgacgatgt cgagaagctg tccgtcccgt tcagcgaaga ccccaacgac ggcgccatcc
     7261 gcactcccga cggccgtcga ggatacctgt acgtgtcgac gcttgagtac gacgccccgg
     7321 ccgggaccat gaccgacacc acgtccggca cggtctaccg cgacaccggc gtcggtgcgt
     7381 tcaccgctga gggcggcgag cagctgctgc ccggctggca gatcacggtg ggcttcgaca
     7441 acttcctgcg ggcggtgacg gatgcctcga tccgcgggcc gctgatctcg gtgacgatct
     7501 ggacgttcgt gttcgcgctg atctcggtgg cgtccacgtt cttcctggga ctgctgctgg
     7561 ccctggtgtt caacaacacc cggatgcgat tccgcaacgg ctaccgcatc ctgctcatcc
     7621 tgccctacgc gttcccggcg ttcctgtcgg cgctggtctg ggcgggcatg atgaacgaga
     7681 gcttcggctt cctcaaccag gtggtcttcg gcggggcatc catcccctgg ctgaccgacc
     7741 cgacgctggc gaaggtgtcc gtgctgatcg tgaacctgtg gctgggcttc ccgtacatgt
     7801 tcctggtgtg catgggcgcg ctgcagggca tcccgtccga cgtcaacgag gccgcctcga
     7861 tggacggcgc gaacccgtgg cagatcttcc gcagcatcaa gctgcccctg ctgctggtca
     7921 ccgtggcacc gctgctgatc tcgtcgttcg cgttcaactt caacaacttc aacctgatct
     7981 acatgctcac caacggcggt ccccgcttcg atgacgtgtc gatccccgtc gggcacaccg
     8041 acatcctgat ctccatggtc tacaaggtgg cgttcaccgg tcagacccgc gactacggtc
     8101 tggcatcggc gttcacgatc ctgatcttca tcgtcgtcgc ggcgatctcg atcatcagct
     8161 tccgcaagac caaggccctc gaggagctga actgagatga gcaccgcttc ctccacccgc
     8221 gacctgcgcc ccgcccgccg ctcgttcggc aagtggttcg gcgacaccgg ctggcgacac
     8281 gtcgtcgcga tcatcgtgag cgcgttcgcg gtgttcccgc tgctgtacgt ggtctccgcc
     8341 tcgctgaacc cgaagggcac cctcaccgga tcgaaccggc tgttctccgc cgtcggcatc
     8401 gacagctacg tgcgcatcct caccgacccg aagaacccct acccgatgtg gttctggaac
     8461 accctcgtca tcgcgacggt gaccggattc gtgacggtct tcctgggtgc tctggccgcg
     8521 tacgcgttct cgcggatgcg gttcaccggt cgccgtgtgg gcctcgtcac catcgtggtg
     8581 gtgcagatgt tcccgcagct gctggccgtg gtcgcgatct tcctgctcat gtcgacgctg
     8641 ggggactggt tccccgcgat cggcctgaac acgcacaccg gcctgatcct cgtctacctc
     8701 ggcggcgcgc tgggcgtgaa cacgtacctg atgtacgggt tcttcaacac gctgccgatg
     8761 gagctcgatg aggccgcccg catcgacggc gcaggtcacg cccggatctt cttcacgatg
     8821 atcctgccgc tggtcgcgcc gatcctcgcg gtcgtcgccc tcctgtcgtt catcggcacg
     8881 gtcaacgagt acgtcatcgc cagcatcatg ctgatcgacc ccgacaagca gacgctcgtg
     8941 gtcggactga ccaagctcgt cgccaatccg cgctacgcgg actggtcggc gttctccgcg
     9001 ggcgccgtga tggcggccgt cccggtgatg gtgctgttcc tgctgctgca gaagtacatc
     9061 gtcggcggac tcaccgcggg tgcggtcaag tagggcagga tgcctgtcat gaccctcccg
     9121 catcacgacg gctcaccgct cttcgtctcg gacgacgccc ccgctctcgg cgacacggtc
     9181 gccgtgcggc tgcgggtgcc tgcggggtac ggcccgctga gggcggtgcg cacccggtcc
     9241 aaccccgatc acgagccgga gtggacggat gccgtgctgc tcggcgccgc cgacggctgg
     9301 gactggtggg aggcacccat ccacgtgcgc aacccgcggc acgggtaccg cttcgtgctg
     9361 ctgcacgagg acggccgagt ggaatggctg aaccagaccg gcatccacac cggcgagaca
     9421 ctggacgccg aggacttcgc gctcgcagcc acccccgccc cgccggcatg gctgcacggc
     9481 tccgtgatgt accaggtgtt cccggatcgc ttcgcgcggt ccgcgcaggc cgacgcgcac
     9541 cccactccgg actgggcgat caccgcgtcc tgggacgatc cggtcgatcc ggtggtcccg
     9601 gggcgctcgc agcagttcta cggcggagac ctgccgggca tcgtcgagca cctcgaccac
     9661 ctggtcgcgc tcggcgtgga cctgctctac ctgacgccgg tgttccccgc ggggtcgaac
     9721 caccgctacg acgcatccag cttcgacacg atcgacccgc tgctcggcgg cgaggacgca
     9781 tacgtcaagc tgatcgaaga ggcgcacgcc cgcggcatcc gcgtgatcgg cgacctgacc
     9841 agcaaccact ccggcgaccg gcacgaatgg ttccagcagg cgctcgggca cccgggcgcc
     9901 gagaccgaga gcttctacta cttcacggat gccgacaaca cgacctacga gtcgtggctg
     9961 ggaacgccga cgctgccgaa gttcaactgg tccagcgacg cgctgcgtga gcggttcatc
    10021 accgccgagg actcggtggt ggcgaagtgg ctgaagccgc cgtactcgat cgacggctgg
    10081 cgcatcgatg tggccaacat gaccggccgc ctgggcgcgg tcgacctgaa cgccgaggtg
    10141 cggcagctgc tcgccgacac cgtgcgggcc atcaaccccg acgcgatcgt gctgggggag
    10201 tcgaccaacg acgcggccac cgacctgcag ggcgacggct ggcacggcgc catgacctat
    10261 ccggcgttca cccgcccgct gtggggctgg ctgagcgagc cgaccggtgc gccgtatctg
    10321 acctacgacg gcgagcgggc gaccgagccg tggttcttca cgcagccgat cggcggcatc
    10381 ccgcgctaca cggcacggga cttcgcggat gccgtgaccc gcttcacggc gggcatcccg
    10441 tggcgggtgc ggctggggaa catgcagccg ctcgacacgc acgacaccgc gcgcttcgcg
    10501 accaacgccg cgccgggagc actgccggtg gcggtcgggc tgtcgatgac catgcccggg
    10561 gtgccggtcg tcttcgccgg cgacgagttc gggctcaccg gcgccgacgg cgagagcagc
    10621 cgcacgccga tgccgtggtc acggcaggac gagcctgagc tcgccgaggt gttcgagacc
    10681 tatcgctcgc tggtcgggct gcgccgtgcg caccccgtgc tggggacggg cgggatgcgc
    10741 tggctgcacg tcgacgatga gaccgtcgtc ttcgttcgtg agagcgccga ggagagcgtg
    10801 ctcgtgctcg cgacccgcgg gggagcggac atcgagctcg cgcccggcgc gctgcccggt
    10861 gcagc
//
LOCUS       DATMSX010000245         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3901,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000245 DATMSX010000000
VERSION     DATMSX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3901"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..879
                     /locus_tag="VN041_07475"
     CDS             166..879
                     /locus_tag="VN041_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HWU28919.1"
                     /translation="MTDAHPDEPHGAGLGQRLNWLRAGVLGANDGIVSVASLVVGVAG
                     ATTDNAALLIAGLAGLVGGAISMALGEYVSVSSQRDTERALIHKEREELRTMPEQELD
                     ELTQLYRDRGLSDETARQVAVELTEHDALAAHLEVELGIDQDDLVNPWHAAISSAISF
                     TIGALLPLLAILLPPPAVRVPITFLAVLVALALTGYVSAKVGGASPVRASLRLVIGGA
                     LALLATWLIGTLLGTTGVV"
     gene            complement(937..1587)
                     /locus_tag="VN041_07480"
     CDS             complement(937..1587)
                     /locus_tag="VN041_07480"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28920.1"
                     /translation="MPDPTAGAALPAVVDAQPGRSPSGALAPRPRRRGDALIDAIHAA
                     VLAELTEGGYASLTMERVAERAGAGKASLYRRWNSRAELVRDTAYHSLRDADGAPDTG
                     SLRGDLVAALSHTAALLTGPLGAALRAVLSETLADDRVEMRELSLGRGRRLMHDIVQR
                     AVSRGEVRADAVTDLRLDVGQALLRDRFLFRDSAIDAETAVRIVDEVLIPLLTDAR"
     gene            complement(1580..>1877)
                     /locus_tag="VN041_07485"
     CDS             complement(1580..>1877)
                     /locus_tag="VN041_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWU28921.1"
                     /translation="AGGGTATGPARIIREAADFHRLQQGDVLVCPYTNPAWTPLFLRA
                     SAVVVDTGSIASHAAIVAREYGLPAVMGTGDGTRVLVDGERITVDGTTGTVTRA"
BASE COUNT          260 a          669 c          683 g          265 t
ORIGIN      
        1 gcgccgcggg cgggaccggc gcctccgggg cggtgggcgc ggtgccctgg cccggtgccg
       61 gggaagtcgg attcgtggtc atggcatcca cgctacggtc atgaggaaat cgttgacacg
      121 gggctgacca gagaccccga ttcgggtttt ccctacactg gcaccatgac ggacgcgcat
      181 cccgacgaac cgcacggagc gggcctcggt cagagactga actggctgcg agcaggcgtc
      241 ctcggcgcca acgacgggat cgtctcggtg gcatccctgg tcgtgggtgt cgcgggtgcg
      301 acgaccgaca acgcggcgct gctgatcgcg ggtctcgccg gcctggtcgg cggcgcgatc
      361 tcgatggcgc tcggcgagta cgtgtcggtg agcagccagc gcgacacgga acgggcactg
      421 atccacaagg agcgcgaaga gctgcgcacc atgccggagc aggagctcga cgagctcacc
      481 cagctctacc gcgaccgcgg cctgagcgat gagaccgcac ggcaggtggc ggtggagctc
      541 accgagcacg atgcgctggc cgcacacctc gaggtggagc tcggcatcga tcaggacgac
      601 ctggtcaacc cgtggcacgc cgcgatctct tcggcgatct cgttcacgat cggggcgctg
      661 ctgccgctgc tggccatcct gctcccgcct cccgccgtgc gggtgccgat cacgttcctc
      721 gccgtgctgg tcgccctggc cctcaccggc tacgtctcgg cgaaggtcgg cggggcgagc
      781 ccggtccggg cctcgctgcg cctcgtcatc ggcggtgccc tcgcgctgct cgcgacctgg
      841 ctgatcggca cgctgctggg gacgaccggg gtcgtctagc ccccggatcc gctcgctctt
      901 caggcatccg ctccatcgat ctgtagcgga tgccgttcag cgggcgtcgg tgagcagggg
      961 gatcagcacc tcgtcgacga tccgcacggc ggtctcggcg tcgatcgcgc tgtcgcggaa
     1021 caggaacctg tcgcgcagca gcgcctgccc gacgtcgagg cgcaggtccg tcacggcatc
     1081 cgcccggacc tctccccgcg agacggcgcg ctgcacgatg tcgtgcatca gccggcggcc
     1141 ccgtccgaga gacagctcgc gcatctcgac ccggtcgtcg gcgagcgtct cggacagcac
     1201 cgcccgcagt gccgcgccga gaggaccggt cagcagcgcc gcggtgtggg agagcgcggc
     1261 gaccaggtct ccgcgcaggg atccggtgtc gggggcgcca tcggcgtcgc gaagggagtg
     1321 ataggcggtg tcgcgcacca gctcggcccg cgagttccag cggcggtaga gggatgcctt
     1381 gcccgccccc gcacgctcgg cgacccgctc catggtgagc gacgcgtatc cgccctcggt
     1441 cagctccgcg agcacagccg cgtggatcgc atcgatcagc gcgtcaccac ggcgacgcgg
     1501 tcgtggcgcc agggccccgg acggcgagcg tccgggctgg gcatccacca ccgcgggcaa
     1561 cgcggcgccg gccgtgggat caggcacggg tcaccgtccc ggtggttccg tcgaccgtga
     1621 tccgctcgcc gtcgacgagc acccgggtgc cgtcgccggt gcccatcacg gcgggcagac
     1681 cgtactcgcg cgcgacgatc gcggcgtgcg aggcgatcga tccggtgtcg acgaccacgg
     1741 cggatgcccg caggaacagc ggtgtccagg ccggattcgt gtacgggcac accagcacgt
     1801 cgccctgctg caggcggtgg aagtcagccg cctcccggat gatccgcgcg ggcccggtcg
     1861 cggtgccgcc acccgcg
//
LOCUS       DATMSX010000246         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13067,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000246 DATMSX010000000
VERSION     DATMSX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13067"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /gene="xseA"
                     /locus_tag="VN041_07490"
     CDS             complement(<1..1054)
                     /gene="xseA"
                     /locus_tag="VN041_07490"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWU28922.1"
                     /translation="MTVFEAAALPGEPPPADSVAPRDSTPDAPTSVARLNGTIRDFIA
                     RWNTVWVEGEITSWNLRAGNVFARLKDVRSDAQISVRVWSSVRARIPADLAVGDHVVA
                     AVKADYFVKGGDFSFIVSAMKHVGLGDQLERLEKLRAQLRQEGLFDASRKKELPFLPH
                     CIGLITGEKSDAEKDVLRNAELRWPQVHFRTAHVAVQGDRSVPELLAALKRLDADPEV
                     DVIIIARGGGDPQTLLGFSDERLVRAVAAASTPVVSAIGHENDHPLLDDVADLRASTP
                     TDAAKRVVPDVGEQRALIAQLRARATSRLTQRISHDIAQLEQLRSRPVLRTPDPIIDD
                     RAQEVWLLASRGRDTLT"
     gene            1194..2183
                     /locus_tag="VN041_07495"
     CDS             1194..2183
                     /locus_tag="VN041_07495"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWU28923.1"
                     /translation="MDGAKRVLLAAPRGYCAGVDRAVVAVEKALERYGAPVYVRKQIV
                     HNIHVVTELEKKGAIFVDEVDEVPEGAHVVFSAHGVAPAVVNAAFDRGLHAIDATCPL
                     VTKVHREAVRFARDDFEILLIGHDGHEEVEGTAGEAPDHVTIVNSPDEADTVVVKDPS
                     KVVWLSQTTLSVDETMETVNRLRTRFPELHNPPSDDICYATQNRQVAIKKVAKDADLV
                     IVVGSANSSNSVRLVEVALEHGAKAAYRVDYADEVKQEWLDGVRTVGVTSGASVPEVL
                     VREVLEALSEAGYGDVEEVRTAEEDLMFSLPKELRQDAAGKRDERALGGRSGH"
BASE COUNT          367 a          805 c          835 g          337 t
ORIGIN      
        1 gggtgagggt gtcgcggccg cgggatgcca gcagccacac ctcctgggcg cggtcgtcga
       61 tgatcggatc gggcgtgcgc agcacggggc gcgagcggag ctgctcgagc tgggcgatgt
      121 cgtgcgagat ccgctgagtg agacggctgg tggcgcgggc gcgcagctgg gcgatcagtg
      181 cgcgctgctc gcccacgtcc gggacgacgc gcttcgcggc atccgtcgga gtcgatgccc
      241 gcagatcggc gacgtcgtcg agcagggggt ggtcgttctc atgtccgatc gcgctgacga
      301 cgggagtgga tgctgcggcc acggcgcgca cgagtcgttc gtcgctgaag ccgagcagcg
      361 tctgcggatc accgccgccg cgcgcgatga tgatcacgtc gacctcgggg tcggcatcca
      421 gccgcttcag cgcggcgagc agctcgggca cgctgcggtc gccctgcacg gccacgtgcg
      481 cggtgcggaa atgcacctgc ggccagcgca gctcggcgtt gcgcagcacg tccttctccg
      541 catccgactt ctcgcctgtg atcaggccga tgcagtgcgg caggaacggg agctccttct
      601 tgcggctcgc gtcgaacagt ccctcctgac gcagctgcgc ccgaagcttc tcgagccgct
      661 ccagctggtc gcccaggccg acgtgcttca tggccgagac gatgaagctg aaatccccgc
      721 ccttgacgaa gtagtccgcc ttcaccgcgg cgaccacgtg atcgccgacg gcaaggtcag
      781 cggggatgcg cgcacgcacg ctcgaccaca cgcggacgga gatctgcgcg tccgagcgca
      841 cgtccttgag ccgcgcgaac acgttgcccg cgcgcaggtt ccacgaggtg atctcgccct
      901 cgacccacac cgtgttccag cgcgcgatga agtcgcggat cgtcccgttc aggcgggcga
      961 ccgatgtcgg ggcatccggc gtggagtcgc gcggcgcgac cgaatccgcg ggcggcggct
     1021 cgccggggag cgcggcggct tcgaagacgg tcatccctcc attgtcccgg atgccgccga
     1081 cacggccctt catcaggcgc cctcggtaga cttcaagggt gagtgcaacc atcgtcaggc
     1141 ttcccgagcc cccgcgccca gcccgtcgat cggcgcccct ccaggaccgt cccgtggacg
     1201 gagcgaagcg ggtgctgctg gcggccccgc gaggctactg cgcgggcgtc gaccgcgccg
     1261 tcgtcgcagt cgagaaggcc ctcgagcgct acggcgcgcc ggtgtacgtg cgcaagcaga
     1321 tcgtgcacaa catccacgtc gtgaccgagc tcgagaagaa gggcgcgatc ttcgtcgacg
     1381 aggtcgacga ggtgcccgag ggcgctcatg tcgtcttcag cgcgcacggt gtcgcgccgg
     1441 ccgtggtgaa cgcggcattc gaccgcggcc tgcacgcgat cgacgcgacc tgcccgctgg
     1501 tgaccaaggt gcaccgcgaa gccgtgcgct tcgcacggga tgacttcgag atcctgctca
     1561 tcggccacga cggccatgag gaggtcgagg gcaccgcggg cgaggccccc gaccacgtga
     1621 ccatcgtgaa ctccccggac gaggcggaca cggtcgtcgt caaggatccg agcaaggtgg
     1681 tctggctgtc gcagaccacg ctctccgtcg acgagaccat ggagacggtc aaccggctcc
     1741 gcacccgctt cccggagctg cacaacccgc cgtccgacga catctgctac gccacccaga
     1801 accgtcaggt cgcgatcaag aaggtcgcga aggacgccga tctggtgatc gtggtcgggt
     1861 ccgcgaactc gtccaacagc gtccgcctcg tcgaggtcgc cctcgagcac ggggcgaagg
     1921 ccgcctaccg tgtggactac gccgacgagg tgaagcagga atggcttgac ggagtgcgga
     1981 cggtcggcgt cacgagcgga gcctcggtgc ccgaggtgct cgtccgtgag gtgctcgagg
     2041 ccctgagcga ggccggctac ggcgatgtcg aagaggtgcg cacggccgag gaggacctga
     2101 tgttctcgct ccccaaggaa ctgcgccagg atgccgcggg caagcgcgac gagcgcgccc
     2161 tgggcggacg ctcgggccac tgacctcgca gggccgcggt cccgcgctgt caccgtctcg
     2221 cccgacggcg gcgttcacat ccccgcccaa ggcccgggcg gtagcgtgga tgccatgagt
     2281 gtcgagcggc cgcagtacgg cgaatacgca acgcccgagg agcagcgtgc gcgcgcaggc
     2341 ctgc
//
LOCUS       DATMSX010000247         6833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13094,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000247 DATMSX010000000
VERSION     DATMSX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13094"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VN041_07500"
     CDS             complement(<1..903)
                     /locus_tag="VN041_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28924.1"
                     /translation="MTPQITAARIVVPEDAPRTVWMLERGEGPTASIISDIAHGGIKT
                     WRRVLEQMMNGSTFRGNRATAAGQSREDALLDEAADQLHTVTPNRALWASLENDLHRA
                     TPDGIGANSDGSIVAVEVKSHEHGYTFAGIPRDHMEQNQWQQHVLGAVSGLYGYEIRD
                     EDDQPPADGATWIDVPRDDEMIAFLVYRANLFIAWRDAGCPDIDDLPDDVAAALDTWA
                     PLKHELDALTKAEKKAADALKKAIAQSMPHAERFGAVGIREAGGFQVSVSETTGIDED
                     AFAADSLPLYRGWVADIARAKERAE"
     gene            complement(900..1103)
                     /locus_tag="VN041_07505"
     CDS             complement(900..1103)
                     /locus_tag="VN041_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28925.1"
                     /translation="MSARIDYVAEAKVQATLASVEQQRTANMQFEWAMLCQRSGRRLG
                     EDAAQAHSEGDALDTLIREGLGL"
     gene            complement(1100..1249)
                     /locus_tag="VN041_07510"
     CDS             complement(1100..1249)
                     /locus_tag="VN041_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28926.1"
                     /translation="MSSGYVHNIPITPTPATDEDRDAARRMVARNVPDAAERARVEAM
                     LGIAS"
     gene            complement(1246..1602)
                     /locus_tag="VN041_07515"
     CDS             complement(1246..1602)
                     /locus_tag="VN041_07515"
                     /inference="COORDINATES: protein motif:HMM:NF040315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6085 family protein"
                     /protein_id="HWU28927.1"
                     /translation="MSQMDMITALVSEGIQKNLRAHRKYIAAGERRDIADDSASVAIA
                     VLIEMSGGNHHIVRSDGETFTLQHPLTERLTGDLLDCDLLRHIYRGERIPKGRYRVLM
                     NDDDTDVDLIPLKEFE"
     gene            complement(1599..1943)
                     /locus_tag="VN041_07520"
     CDS             complement(1599..1943)
                     /locus_tag="VN041_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28928.1"
                     /translation="MSAPAIITTGDNPRARKSDPKTSHAAADSITPEGREASELEVLA
                     ILADETSPITAEQIESRHVQRAWQGQSNHTYTASRLRTALKQLADDGHVIRDGEGRTK
                     SGRRAATWRVAS"
     gene            complement(1940..2230)
                     /locus_tag="VN041_07525"
     CDS             complement(1940..2230)
                     /locus_tag="VN041_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28929.1"
                     /translation="MSITQITCPGSPATSAEVEDGVVDDVHPAPFAPVPAIHPRDAAA
                     CYICGRGEHPPTDEHAFWSNADADREFNLMPVGPLPSMTAVETLDPREAVFL"
     gene            complement(2286..2585)
                     /locus_tag="VN041_07530"
     CDS             complement(2286..2585)
                     /locus_tag="VN041_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28930.1"
                     /translation="MGTVAPASGTRPAAVAPSGAMVTLRWALLAGMVACGVAVAVTGR
                     WNGVALLQIVFLLAYFATYQNVRAAVARFIGGMDPAPSLSRLDELDGLGRHDHQK"
     gene            complement(2648..2911)
                     /locus_tag="VN041_07535"
     CDS             complement(2648..2911)
                     /locus_tag="VN041_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28931.1"
                     /translation="MSNVTALRVTPEAETESQEGLGFEGLPVVMSPSLLADVLQVTTR
                     TLERWRVDETGPKWFKIPGSSLIRYARVDFIDWIVAARDGSAS"
     gene            complement(2908..3162)
                     /locus_tag="VN041_07540"
     CDS             complement(2908..3162)
                     /locus_tag="VN041_07540"
                     /inference="COORDINATES: protein motif:HMM:NF020251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU28932.1"
                     /translation="MTKQTSPAAVTSRIADEVRAEMARQKKTGAELAAVLGITQHTVG
                     RRLNGATPFNMIELILVAAWLGISLGELIRRAETPLAVAS"
     gene            3248..3595
                     /locus_tag="VN041_07545"
     CDS             3248..3595
                     /locus_tag="VN041_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28933.1"
                     /translation="MEDDEFATRFSDALVSEIKAEMGRQDLSSRGLARLIGSNPQFVS
                     SRLDGGNPRTGERVILNIRDIVQIANALGIPYLDLITRAENVARQRDDYDLVANETIN
                     EFPEGDDADWDQA"
     gene            3609..3917
                     /locus_tag="VN041_07550"
     CDS             3609..3917
                     /locus_tag="VN041_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28934.1"
                     /translation="MSTPRTPLFTGKEIGIVAGSIAGFIVLAIIAAAGGFWTLITLGP
                     LLLVALVLGSIALTVWLFYKFGVIVVRNGVIAANQEIARFERPAAAQPGSEPTQTGLP
                     "
     gene            3917..4360
                     /locus_tag="VN041_07555"
     CDS             3917..4360
                     /locus_tag="VN041_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWU28935.1"
                     /translation="MSLDAWDLAADLGLEVRERRGIHRSGYLPGADYIEITPGMKGRV
                     LRGVIYHEIGHHVLGHRPTEFGLIRKRQEYGANLWAAHRLITPATYAEAEHNRDGRIT
                     GMAIDLNVPDEFIVVFRESLLRTEQVTYVRPRMGVGQWAHKIEVA"
     gene            4360..5691
                     /locus_tag="VN041_07560"
     CDS             4360..5691
                     /locus_tag="VN041_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWU28936.1"
                     /translation="MGRAWVTDRWVKDAVVTMPDGTTTKISATSAQLRSLKTLPEHFR
                     TSKFMKGSRWEVGWHDPTAAGRRQVKKLFARRSDADAFAAELEDDIRSDRYIDPASRA
                     RTYRAVAEAWLASKNRIKDSSYFRYRRELDNYVLPKWGERTIGSITRTDIDEWILQLR
                     DGTAVHDFDRTEYLKQTKARKPRTPVKMKPSYLRHVASATFGGPLRYAVREGWIGRDP
                     FRNVELPRDEGDLESDLPSLSYQDIDALADAALEITDNESDRWLVQTLAVSGPRIGEA
                     TALKIKDLDLSNKRVRIHRTWTTDREGKRKLGPVKTWEKRWLPLADFIVSGLKAIIKD
                     RDPEDYVFTSPRGAAVDGRNWRNRVWEKAQGAVGLTQHLSVHDLRHVAATNAIAAGAD
                     VKLVQQMLGHKDATETLNTYSHLWPNRVAEVIAKIEKRRRKALKNAHDKAA"
     gene            complement(5828..5904)
                     /locus_tag="VN041_07565"
     tRNA            complement(5828..5904)
                     /locus_tag="VN041_07565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5868..5870),aa:Met,seq:cat)
     gene            complement(5938..6009)
                     /locus_tag="VN041_07570"
     tRNA            complement(5938..6009)
                     /locus_tag="VN041_07570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5975..5977),aa:Thr,seq:ggt)
     gene            complement(6122..>6833)
                     /locus_tag="VN041_07575"
     CDS             complement(6122..>6833)
                     /locus_tag="VN041_07575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWU28937.1"
                     /translation="ASLGVMGVAGMIAVSMTLPASAVAAVQNNGSGATASLVASAAPA
                     KKVADDEIQAYVASAAVEDEDLAREGEYSTVSLLDVAAEEGIKRVSNSLYTNDPNAAI
                     QWPFMVGVAMSSPYGMRNGRMHAGIDLVPGAGATIQAIADGTVRIATESGGAYGVTVY
                     VDHVIDGQVVTSHYAHMQHGSLAVKTGQKVKVGDTIGKVGNTGRSYGAHLHFEIIING
                     STVDPLAWMRKNAGRYSY"
BASE COUNT         1105 a         2339 c         2269 g         1120 t
ORIGIN      
        1 ctccgcgcgc tccttggcgc gcgcgatgtc ggccacccag ccgcggtaca gcggcagcga
       61 gtcggcagcg aacgcgtcct cgtcgatgcc cgtggtctcg gacacgctca cctggaagcc
      121 gccggcctcg cggatgccga ctgcgccgaa ccgttcggcg tgtggcatgc tctgagcgat
      181 cgccttcttc agcgcgtcgg cggccttctt ctctgccttc gtgagggcgt cgagctcgtg
      241 cttcagcggg gcccacgtgt cgagcgcggc ggcgacgtcg tcggggaggt cgtcgatgtc
      301 ggggcatccg gcgtcccgcc atgcgatgaa caggttggcg cggtagacga ggaacgcgat
      361 catctcgtcg tcgcgaggga cgtcgatcca tgtggcgccg tcggcgggcg gctggtcgtc
      421 ctcgtcgcgg atctcgtacc cgtacaggcc ggacaccgcg ccgagcacgt gctgctgcca
      481 ctggttctgc tccatgtggt cgcgcgggat ccctgcgaac gtgtagccgt gctcgtggga
      541 cttgacctcg accgccacga tcgagccgtc cgagttcgcg ccgatgccgt ccggggtcgc
      601 ccggtgcagg tcattctcga gcgacgccca gagggcccgg ttcggggtga cggtgtgcag
      661 ctggtcggcg gcctcgtcga ggagggcatc ctcgcgggac tggccggcgg ccgtggcgcg
      721 gttcccgcgg aacgtgctgc cgttcatcat ctgctcgagc acgcgccgcc acgtcttgat
      781 gccgccgtgc gcgatgtccg agatgatcga cgccgtcgga ccctcgccgc gctcgagcat
      841 ccacacggtg cgcggggcgt cctccgggac cacgatgcgg gccgcggtga tctgcggggt
      901 cacagtccca gcccctcacg gatcagggtg tcgagcgcgt cgccctcgga gtgcgcctgt
      961 gccgcgtctt cgccgaggcg gcgtccggat cgctgacaca gcatcgccca ctcgaactgc
     1021 atgttcgcgg tgcgctgctg ctcgaccgat gccagcgtgg cctgcacctt cgcctcggcg
     1081 acgtagtcga tgcgggcgct catgatgcga tccccagcat cgcctcgacc cgcgcgcgct
     1141 cggccgcatc cgggacgttt cgggcgacca tgcggcgggc cgcgtcgcgg tcctcgtccg
     1201 tcgccggggt cggcgtgatc gggatgttgt gcacgtaccc gctgctcatt cgaactcctt
     1261 cagggggatc aggtcgacgt cggtgtcgtc gtcgttcatc aggacccggt agcggccctt
     1321 cgggatccgc tcgccgcggt agatgtggcg cagcaggtcg cagtcgagga ggtccccggt
     1381 caggcgctcg gtgagcgggt gctgcagagt gaacgtctcc ccgtcggagc ggacgatgtg
     1441 gtggttgccg ccggacatct cgatcagcac ggcgatcgca acggatgcgg aatcgtcggc
     1501 gatgtcccgc cgctcaccgg cggcgatgta cttccggtgc gcgcgcaggt tcttctggat
     1561 gccctccgac accagggccg tgatcatgtc catctggctc atgacgccac ccgccatgtc
     1621 gcggcgcggc ggccggactt cgtgcggccc tcaccgtcac ggatcacgtg cccgtcgtcg
     1681 gcgagttgct tcagcgcggt ccgcagacgg gatgccgtgt acgtgtggtt ggactggccc
     1741 tgccatgcgc gctgcacgtg gcgggactcg atctgctccg ccgtgatcgg ggaggtctcg
     1801 tcggcgagga tcgcgagcac ttccagctcg gatgcctccc ggccctccgg ggtgatcgag
     1861 tccgccgcgg cgtgcgacgt cttcgggtcg ctcttgcggg cccgggggtt gtctcccgta
     1921 gtgatgatcg cgggggcgct cacaggaaca ccgcctcacg cgggtcgagc gtctcgaccg
     1981 cggtcatgct cgggagcgga ccgacgggca tcaggttgaa ctcgcggtca gcgtcagcgt
     2041 tcgaccagaa cgcgtgctca tctgtcggtg ggtgctcccc gcggccgcag atgtagcatg
     2101 cggccgcgtc gcggggatga atcgcaggga ccggcgcgaa cggagcgggg tgcacgtcat
     2161 cgacgacgcc gtcctctact tccgcactgg tggccggcga gccggggcag gtaatctggg
     2221 tgatggacat ggttggatgt cctttctctt gatggccggt cccgttgcag cggggccggc
     2281 tttcttcact tctggtggtc gtggcggccg aggccgtcga gctcgtcgag acgcgacagg
     2341 ctcggagcgg ggtccatgcc gccgatgaag cgcgccaccg cggcgcgcac gttctggtag
     2401 gtcgcgaagt acgcgagcag gaagacgatc tgcagcagcg cgacgccgtt ccagcggcca
     2461 gtgaccgcga cggcgacacc gcaggcgacc atgcctgcga gcagcgccca ccgaagcgtc
     2521 accatggcgc cggaaggcgc gaccgccgcg ggccgcgttc cggatgccgg ggcaaccgtc
     2581 cccacggtgc cccggccatc cgagcccgaa accccagcgg gcacccggtg ctccccagta
     2641 ccggagatca tgatgcgctc ccgtcgcggg ccgcgacgat ccagtcgatg aagtcgacac
     2701 gggcgtagcg gatcaggctg gacccgggga tcttgaacca cttcggcccg gtctcgtcca
     2761 cgcgccagcg ctcgagcgtg cgggtcgtga cctgcagcac gtcggcgagg aggctcgggg
     2821 acatcacgac cgggagcccc tcgaatccca ggccctcctg ggattcggtt tcggcctcgg
     2881 gtgtcactcg cagcgcagtc acgttgctca cgacgccacc gccagcgggg tctctgctcg
     2941 ccggatcagc tcgccgaggc taatccccag ccacgccgcc acgaggatca gctcgatcat
     3001 gttgaacgga gtcgcgccat tcaggcgacg ccccactgtg tgctgggtaa taccgagcac
     3061 cgctgcgagt tctgcgccgg tcttcttctg gcgtgccatc tcggcacgca cctcatcggc
     3121 gattcgggaa gtcacagcag cgggggatgt ctgctttgtc atacagccat cgtgggtgac
     3181 aaatcatcca ctgtcaagct gatcattcat attgacgaaa aatcattcag ggtgcatgct
     3241 tggacgcatg gaagacgacg agttcgccac acgcttctcc gacgccctgg tctccgagat
     3301 caaggcggag atggggcgcc aggatctctc atcgcgcggg ctcgcgcgcc tgatcggctc
     3361 gaacccacag ttcgtgtcat cccgcctcga cggtgggaac ccgcgcacgg gcgagcgggt
     3421 gatcctcaac atccgtgaca tcgtccagat cgcgaacgcg ctcggcatcc cctacctcga
     3481 tctgatcacc cgcgccgaga atgtcgcacg ccaacgcgat gattatgacc tcgtcgcgaa
     3541 cgagaccatc aacgagttcc cggaaggcga cgacgccgac tgggaccagg cataaccaag
     3601 gagccatcat gtctacgccc cgcacaccct tgttcactgg caaggagatc ggcatcgtcg
     3661 ccggttcgat cgccgggttc atcgtgctcg ccatcatcgc cgcggcgggc gggttctgga
     3721 ccctcatcac cctcggacca ctcctgctcg tggcgctggt ccttgggtct atcgcgctca
     3781 ccgtctggct gttctacaag ttcggcgtca tcgtcgtccg caacggagtg atcgcagcga
     3841 accaggagat cgcgcgcttc gaacggcccg cggccgcgca gcccgggtcc gagcccaccc
     3901 agacaggcct cccctgatgt cgctcgacgc atgggacctc gcggccgatc tcgggctcga
     3961 ggtccgcgaa cgccgcggca tccatcgcag cggctacctc ccgggcgcgg actacatcga
     4021 gatcaccccc ggcatgaagg gccgagtcct ccgcggcgtc atctaccacg agatcgggca
     4081 ccacgtcctc ggccaccgcc ccacggagtt cggactcatc cgcaagcgcc aggagtacgg
     4141 cgccaacctc tgggccgcac acaggctgat cacgcccgcc acctacgccg aagccgagca
     4201 caaccgagac ggacgcatca caggcatggc gatcgacctc aacgtccccg acgagttcat
     4261 cgtcgtgttc cgcgagtccc tgctccgaac tgagcaggtc acctacgtcc ggccccgcat
     4321 gggcgtcggc cagtgggccc acaagatcga agtcgcctga tgggccgcgc gtgggtcacc
     4381 gatcgatggg tgaaggacgc cgtcgtcacc atgcccgacg gcacgaccac caagatcagc
     4441 gcgacgagcg cccagctgcg gtcactgaag accctgcccg agcacttccg gacgtcgaag
     4501 ttcatgaagg gatcgcgctg ggaggtcgga tggcacgacc ccacggccgc cgggcggcgg
     4561 caggtgaaga agctcttcgc gcgacgcagc gacgccgacg ctttcgcggc agagctcgag
     4621 gacgacatcc gctccgaccg gtacatcgac ccggcatccc gcgctcgcac gtaccgtgcc
     4681 gtcgccgagg cctggctcgc gtcgaagaac cgcatcaagg actcgtcgta cttccgctat
     4741 cgtcgcgaac tcgacaacta cgtgctcccg aagtggggtg agcggacgat cgggagcatt
     4801 acccgaaccg acatcgacga gtggatcctg cagctgcgcg atggaaccgc cgtgcacgac
     4861 ttcgaccgca cggaatacct gaagcagacg aaggcacgca agccgcgcac accggtgaag
     4921 atgaagccct cgtacctgcg ccacgtggca tccgccacct ttggcggccc gctgcgctat
     4981 gccgtgcgcg agggctggat cggccgcgac ccgttccgca acgtcgaact cccccgcgac
     5041 gagggcgacc tagagtcgga ccttccttcg ctgagctacc aggacatcga cgccctcgcg
     5101 gacgccgcgc tcgagatcac cgacaacgag tccgaccggt ggctcgtgca gacgctcgcc
     5161 gtcagcggtc cgcgcatcgg cgaagcgacc gctctgaaga tcaaggatct cgacctgtcg
     5221 aacaagcggg tccgcatcca ccgcacctgg accaccgacc gcgaaggcaa gcgcaagctc
     5281 ggcccggtga agacgtggga gaagcggtgg ctgccgctgg ccgacttcat cgtctccggg
     5341 ctcaaggcca tcatcaagga ccgcgacccg gaggactacg tcttcaccag cccgcgtggc
     5401 gcggcagtcg acggccgcaa ctggcgcaac cgggtatggg agaaggcgca gggcgccgtg
     5461 ggccttacgc agcacctctc cgtgcacgac ctgaggcacg tcgcggcgac gaacgcgatc
     5521 gccgccggcg cggacgtgaa gctcgtgcag cagatgctcg gccacaagga cgccaccgag
     5581 acgctgaaca cgtactcgca tctctggccg aaccgcgtcg ccgaggtcat cgccaagatc
     5641 gagaagcgcc ggcgcaaggc cctcaagaac gcacacgaca aggccgcctg accccgcaac
     5701 gacgaacgcc ccgccgctct tacgagtggc ggggcgtttg caatgtgaga cgaatgtgag
     5761 acggcggccg atatgagact ctcaggaaac gaaaagcccc cggaatctcg cgggattccg
     5821 ggggttctgg tagcaggagc ggggcttgaa cccgcgacct cacgattatg agtcgtgcgc
     5881 tctcaccaac tgagctaccc tgccgcagcg catccgcttc gaaagtcgaa tgctgcgagc
     5941 cccgagtcag gattgaactg acgacccctt ccttaccatg gaagtgctct gccactgagc
     6001 tatcggggcg tgctgtcctc ttcaggacaa ccagatgagc ttatcatccg cgacggccag
     6061 ccacgaaatc gtgacggcag ttcgcgtcgg ccgggcgtgc ctgcgcggca gacgcgagcc
     6121 gtcagtacga gtaccggccc gcgttcttgc gcatccacgc aagcgggtcg actgtcgaac
     6181 cgttgatgat gatctcgaaa tgcaggtgcg caccgtagga gcggccggtg ttgccgacct
     6241 ttccgatggt gtcccccacc ttgaccttct ggccggtctt gaccgccagc gagccgtgct
     6301 gcatgtgcgc gtagtggctc gtgacgacct ggccgtcgat cacgtgatcc acgtacacgg
     6361 tcacgccgta ggcgccgccg gactcggtcg cgatgcggac cgttccgtcg gcgatcgcct
     6421 ggatcgtggc acctgcaccg ggcacgaggt cgatgcccgc gtgcatgcgg ccgttgcgca
     6481 tgccgtaggg cgagctcatg gccacaccga ccatgaacgg ccactggatc gcggcgttcg
     6541 ggtcgttggt gtagaggctg ttggagacgc gcttgatgcc ctcttcagca gcgacgtcga
     6601 gcagcgagac cgtggagtac tctccctcac gagccaggtc ctcgtcctcc accgcggcgg
     6661 atgcgacgta ggcctggatc tcgtcgtcgg cgaccttctt ggcgggcgcg gcggacgcga
     6721 cgagcgatgc cgtcgcgccg gagccgttgt tctgcaccgc ggcgaccgcg ctggcgggca
     6781 gggtcatgga cacggcgatc attcctgcga cgcccatgac gccgagggac gcg
//
LOCUS       DATMSX010000248         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_3956,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000248 DATMSX010000000
VERSION     DATMSX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>2159)
                     /locus_tag="VN041_07580"
     CDS             complement(108..>2159)
                     /locus_tag="VN041_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C14"
                     /protein_id="HWU28938.1"
                     /translation="AEGPQNVRRVESVAELAGIRARAGDVAIAAGYRTAGDAGVLVYT
                     GVDAGGVKANGGTVIAAQKGTVWLLQHDGTVDFRAFGILDAKTAADDALDAMVGDESI
                     RRIEAHTDLLFQRRHRFTRSRLEIDFGGHTISTEGIEKNTHDNPFGAVMFFTGVAKDD
                     TIEHALAEAWPELTDAVAVPDAGRFPVGSWWAVQCAPVAGGGGDERELQRFVEVTQHI
                     DDTHIRIDYLNGWPLEKGRRLTWRRFDPVTSVRISNMRFLGAGPFDGPTDGGLPDPRE
                     MTGSHPVAFEYAIRCDVADVHASQTWWPVVMRRWNTHFVTERCSIENPPTVFYGGAGY
                     LTQQIYCLYGRVSDCTSSNARHLNDLTASAYCLVENCHGDGDDQGGNPFTTHGQYEHD
                     LTFLGCSGLMDIANSGAQWGTSAKRITVRDHVCSWFVAGTKITDLTLENVRVIPRSTF
                     DPQATMTINADGAQLRGCTAGLLAIGQRSSRSTRPTTIEDCTFALPKDQVLVQTPVAN
                     TVTFSRCTIGGLDAAKVRGSGAVRFLDCDLSGPASGGPVEIGSSRVTIRGGTVRDAAL
                     VMTAARDQVVVLDGVELTTTRTQGATLGRGAGGGTVSWRLSGVESTASGDAAHLGIGS
                     GVNHVRIQDARFNGGRLDLAEGFAGGSTLLYSASVENGVKRSLPAAGDRILIDDTILT
                     V"
BASE COUNT          298 a          722 c          795 g          344 t
ORIGIN      
        1 acggcgctgc gcggcacgcg gatgcgggcg ccgagggcgg tgccctgacg tgccgccgac
       61 gcgggcgatt gctgcgtgct ctgcatgagt gccgttccgg tggtgggtca gacggtcagg
      121 atcgtgtcgt cgatgaggat acggtcgcct gccgcgggca ggctgcgctt cacgccgttc
      181 tcgacggatg cgctgtacag cagcgtcgag ccgcccgcga agccctcggc aagatcgagt
      241 cgtccgccgt tgaaccgcgc gtcctggatg cgcacgtgat tcacgccgga cccgatgccg
      301 agatgcgccg cgtcgccgga tgccgtggac tccacgccgc tcaggcgcca ggagacggtg
      361 ccgccgcccg cgcctctgcc gagggtcgca ccctgggtgc gggtcgtggt cagttcgacg
      421 ccgtcgagca cgacgacctg gtcgcgcgcc gccgtcatca ccagggcggc gtcgcggacg
      481 gtgccgccac ggatcgtgac tcgcgacgag ccgatctcca ccggtccgcc cgaggcggga
      541 ccgctgaggt cgcagtcgag gaaccgcacg gcgccggagc cgcgcacctt ggccgcatcg
      601 agcccgccga tcgtgcagcg cgagaaggtg acggtgttcg cgaccggggt ctgcacgagc
      661 acctgatcct tcggcagtgc gaacgtgcag tcctcgatcg tcgtgggacg ggtggatcgc
      721 gagctgcgct gcccgatggc gagcaggccg gccgtgcatc cacgcagctg cgcgccgtcg
      781 gcgttgatcg tcatggtcgc ctgggggtcg aaggtcgacc gcgggatcac gcggacgttc
      841 tccagggtga ggtcggtgat cttcgtgccc gcgacgaacc acgagcagac atggtcgcgg
      901 accgtgatgc gcttcgcgga ggtgccccac tgggcgccgg agttcgcgat gtccatcagt
      961 ccggagcatc cgaggaaggt gaggtcgtgc tcgtactgcc cgtgggtggt gaacgggttg
     1021 ccgccctggt cgtcgccgtc gccgtggcag ttctcgacca ggcagtacgc gctggcggtg
     1081 aggtcgttca ggtgccgcgc gttcgaggag gtgcagtcgc tgacccggcc gtataggcag
     1141 tagatctgct gggtgaggta gcccgcgccg ccgtagaaca cggtgggcgg gttctcgatc
     1201 gagcagcgct cggtcacgaa gtgcgtgttc cagcgccgca tcacgaccgg ccaccacgtc
     1261 tgcgaggcgt gcacgtcggc gacgtcgcag cggatggcgt actcgaaggc gaccggatgc
     1321 gatccggtca tctcgcgcgg gtccggaagg ccgccgtcgg tggggccgtc gaagggcccc
     1381 gcgccgagga agcgcatgtt cgagatgcgc acgctggtga ccgggtcgaa ccgacgccag
     1441 gtcaggcggc gtcccttctc gagcggccag ccgttgaggt agtcgatacg gatgtgggtg
     1501 tcgtcgatgt gctgggtgac ctcgacgaac cgctgcagtt cgcgctcgtc ccctccgccg
     1561 cctgcgacgg gggcgcactg cacggcccac caggagccga ccgggaaccg cccggcgtcg
     1621 ggcaccgcga cggcgtcggt cagctcgggc caggcctcgg cgagcgcgtg ctcgatggtg
     1681 tcgtccttcg ccacgccggt gaagaacatc acggcaccga acgggttgtc gtgcgtgttc
     1741 ttctcgatcc cctcggtgga gatcgtgtgc ccgccgaagt cgatctcgag acgggagcgg
     1801 gtgaagcggt gccttcgctg gaagagcagg tcggtgtgcg cctcgatccg gcggatgctc
     1861 tcgtcaccga ccatcgcgtc gagggcgtcg tcggccgccg tcttcgcgtc gaggatgccg
     1921 aaggcgcgga agtcgacggt gccgtcatgc tgcagcagcc acaccgtgcc cttctgcgcg
     1981 gcgatcaccg ttccgccgtt cgccttcacc ccaccggcat cgacacccgt gtagacgagc
     2041 acgccggcat cacctgcggt gcggtagccg gcggcgatcg cgacatcgcc ggcacgtgcg
     2101 cggatgcccg ccagctcggc gacgctctcc acgcgccgca cgttctgcgg accctcggc
//
LOCUS       DATMSX010000249         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_69913,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000249 DATMSX010000000
VERSION     DATMSX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69913"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..973
                     /locus_tag="VN041_07585"
     CDS             98..973
                     /locus_tag="VN041_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWU28939.1"
                     /translation="MADTYVRERSTKGRKDLSYPAAPEPLTVPVYDNHCHLEITDGVE
                     PLSLREQLDRAGAVGIAGVVQASGDIESSRWAVDAAASDPRVLAAVAIHPNDAPTYQQ
                     DGRLDEAIAVIDELAAHPRTRAIGETGLDYFRTDEQGRPAQHESFEAHIALAKKHGIA
                     MQIHDRDAHDDVLETLARVGAPERTVFHCFSGDEAMARTAADAGYWLSFAGNITFKNA
                     QNLRDALHVTPLDRILVETDAPFLTPVPLRGRPNAPYLVPLTMRFMAAELEMDVDELC
                     AQVAANTLAVYGSFE"
     gene            988..1572
                     /locus_tag="VN041_07590"
     CDS             988..1572
                     /locus_tag="VN041_07590"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWU28940.1"
                     /translation="MLFLDEITLEDDHVRLEPLSHEHAEDLGRAAATLAHAWYTSVPT
                     AEGVPAEIDRRLTAREAGSMNPFAVRRLGSGEVIGMTTFCSIDQPNRRVEIGYTWIGA
                     LAHGTEVNPAMKRLMLAHAFERCDAIAVSLMTHFHNRQSRAAIERLGAKLDGVLRNHR
                     ILPDGSLRDTVAYSILPHEWPAVRNGLDARLARY"
     gene            complement(1566..2876)
                     /locus_tag="VN041_07595"
     CDS             complement(1566..2876)
                     /locus_tag="VN041_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28941.1"
                     /translation="MTALGELIAPRRLGRDFRWLMSAAWTSNIGDGIALAASPLLIAS
                     MTSSPVLVASGAMLQFLPWLLFGLHAGAIADRVERRMLVMLAHGVRAVIVLALVAFLV
                     TGTANIAIVLAVSFLYGTAEVFADTSTSTLLPMMVKPADLGIGNARLQAGFLVANQLA
                     GPPLGAFLFAVGHFWPFLVQVLAVSVALVLVARIARQPVPPRASAGKDPSAESEPRKS
                     HVGHDIAEGVGWAWRNKPVRTLIVIILAFNVTWAAPWGILVLYATEHLKMGPVGFGAL
                     TTASALGGLAATLSFGWLERHVSFATLMKICLSLEVLMHLAFALTTNGVVALGIMFGF
                     GAYAFVWGTISTTVRQRLVPHELQGRVGSVNMVGVFGGMVIGQAVGGLIAQAWGLTAP
                     WWFAFGGAALTLLLMWRQISHIAAAKPVLEDAPADESPAGDAAQ"
     gene            2924..3772
                     /gene="rsmA"
                     /locus_tag="VN041_07600"
     CDS             2924..3772
                     /gene="rsmA"
                     /locus_tag="VN041_07600"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWU28942.1"
                     /translation="MTVSLLGATEIRRLAAELDVTPTKKLGQNFVVDANTVRKIVQAA
                     DVQAGERVVEIGPGLGSLTLAILETGASVTAVEIDHRLAERLPQTAAERGVPDGALTV
                     VDADAMRVTELPGDPSVLVANLPYNVSVPVLLHFLETFPHLQRGVVMVQAEVAERLAA
                     KPGSKIYGAPSVKAAWYGPWRLTGTVSRQVFWPVPNVDSLLVGFHRDPEPRGDEIERR
                     RTFQIVDAAFNQRRKMLRQALSGIFGSSAEASAVLEAAGVAPTARGEDLTVDDYHRIA
                     RVRQDG"
     gene            3782..4846
                     /gene="mgrA"
                     /locus_tag="VN041_07605"
     CDS             3782..4846
                     /gene="mgrA"
                     /locus_tag="VN041_07605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HWU28943.1"
                     /translation="MTDPRFRPDVPELHRPYTAASDRYQRFEYRQVGSSGLYLPPISL
                     GLWWNFGDNIPMDDQRALLRHAFDSGITHFDLANNYGPPYGSAEKNFGRIFAEDLKPY
                     RDELIISSKAGYDMWPGPYGDLGSRKYILASAEQSLTRMGLDYVDIFYSHRVDAVTPV
                     EETIGALDTLVRQGKALYVGISSYSAERTEAALAVARDLGTPLVIHQPSYSILNRWVE
                     DGLTDTLRAEGVGAIAFTPLAQGLLTGKYLADGTAERAQKRGSLPDRPLSDEGIAVLR
                     GLNDIAAERGQSLAQMALQWTLRDPVVVSALIGASRPEQLDENIAAVDGPAFTDEELT
                     AIDALAHGIDVNLWSISSDL"
     gene            4854..>5254
                     /locus_tag="VN041_07610"
     CDS             4854..>5254
                     /locus_tag="VN041_07610"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191631.1"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWU28944.1"
                     /translation="MSFARHPDAVHVRAPGKINVYLGVGPRHDDGYHALATVFQAVSL
                     YEDVYASASDGFSIDVVGDVDISGVPLDDRNLALRAAKLLAQTAGVTQGVHLDIRKGV
                     PVAGGMGGGSADAAAALVACDALWGTELGPRG"
BASE COUNT          828 a         1894 c         1799 g          733 t
ORIGIN      
        1 gatccgcgag gccggtgcct ggggtgcgct gaagccgggc accaccgtgt cggcgctcgc
       61 gccgctgttc ccgcgcgtgg agcagaccgc gtagagcatg gccgacacct acgtgcgcga
      121 gcgctccacg aaggggcgca aggacctgag ctacccggcg gcgccggagc cgctcacggt
      181 cccggtgtac gacaaccact gccacctgga gatcaccgac ggcgtcgaac cgctctcgct
      241 gcgcgagcag ctggatcggg cgggcgccgt cgggatcgcc ggcgtcgtgc aggcctccgg
      301 cgacatcgag tcctcgcggt gggcggtgga tgccgcggca tccgatccgc gcgtgctcgc
      361 ggccgtcgcg atccacccga acgacgcgcc gacctaccag caggacgggc gcctcgatga
      421 ggcgatcgcg gtgatcgacg agctcgcggc gcacccgcgc acccgggcga tcggcgagac
      481 cgggctggac tacttccgca cggacgaaca gggccgcccc gcgcagcacg agtccttcga
      541 ggcgcacatc gcgctggcga agaagcacgg catcgccatg cagatccacg accgcgacgc
      601 gcacgacgac gtgctggaga ccctcgcccg tgtcggggcg ccggagcgca ccgtgttcca
      661 ctgcttctcg ggcgacgagg cgatggccag gacggcggcg gatgccggat actggctctc
      721 cttcgcgggc aacatcacct tcaagaacgc gcagaacctg cgcgacgcgc tgcatgtgac
      781 gccgctggat cggatcctgg tggagacgga tgcccccttc ctgacgccgg tgccgctgcg
      841 cggacgtccc aacgcgccgt atctcgttcc gctgaccatg cggttcatgg cggccgagct
      901 cgagatggat gtcgacgagc tctgcgcgca ggtcgcggcg aacacgctcg ccgtctacgg
      961 ctccttcgaa tgacgggtgg ggatcggatg ctcttcctcg acgagatcac gctcgaagac
     1021 gaccatgtgc ggctcgagcc gctcagccac gagcacgccg aggatctcgg ccgggccgcg
     1081 gcgaccctgg cgcatgcctg gtacacgtcg gtcccgaccg ccgagggcgt gcccgccgag
     1141 atcgaccgtc gcctgaccgc gcgggaggcg ggcagcatga acccgttcgc cgtccggcgc
     1201 ctcggcagcg gcgaggtgat cgggatgacc acgttctgca gcatcgatca gccgaaccgt
     1261 cgcgtcgaga tcggctacac gtggatcggc gcgctggcgc acggcaccga ggtcaacccg
     1321 gcgatgaagc ggctcatgct cgcgcacgcc ttcgagcgct gcgatgccat cgccgtgtcg
     1381 ctgatgacgc acttccacaa ccggcagtcg cgggcagcca tcgagcgcct cggcgcgaag
     1441 ctcgacggcg tgctgcgcaa tcaccgcatc cttccggacg gctcgctgcg cgacacggtg
     1501 gcgtactcga tcctgccgca cgagtggccg gcggtgcgca acgggctgga tgcccgcctg
     1561 gcgcgctact gagcggcgtc tccggccggc gactcatcag ccggtgcgtc ctccagcacc
     1621 ggcttcgccg cggcgatgtg cgagatctgc cgccacatca gcaggagcgt gagcgccgcc
     1681 ccgccgaagg cgaaccacca cggcgccgtc agtccccacg cctgcgcgat cagcccgccg
     1741 acggcctgcc cgatgaccat gccgccgaac acgcccacca tgttgaccga tccgacccgc
     1801 ccctgcagct cgtgcggcac cagccgttgg cgcacggtcg tggagatcgt gccccacacg
     1861 aacgcgtagg cgccgaatcc gaacatgatc ccgagcgcca cgacgccgtt cgtcgtcagc
     1921 gcgaaggcca ggtgcatcag cacctccagc gacaggcaga tcttcatcag cgtggcgaac
     1981 gacacgtgcc gctccagcca cccgaagctg agcgtggcgg cgaggccgcc cagagcggat
     2041 gccgtggtca gcgcgccgaa gccgaccggc cccatcttca gatgctcggt cgcgtacagg
     2101 accaggatgc cccagggggc cgcccaggtg acgttgaatg ccaggatgat gacgatcagc
     2161 gtgcgcacgg gcttgttgcg ccacgcccag cccacgccct ccgcgatgtc gtgtccgacg
     2221 tgcgacttcc ggggctctga ctcggctgac gggtccttgc ccgccgatgc gcgcggcggc
     2281 accggctgcc gggcgatccg cgccaccagc accagagcca ccgacaccgc cagcacctgc
     2341 accaggaacg gccagaagtg gcccaccgcg aacaggaagg cgcccagcgg gggccctgcc
     2401 agctggttcg ccaccaggaa gcccgcctgc aggcgggcgt tgccgatccc caggtcggcc
     2461 ggcttcacca tcatcggcag cagcgtgctc gtgctggtgt cggcgaagac ctccgccgtg
     2521 ccgtacagga acgacaccgc cagcacgatg gcgatgttcg cggtgcccgt caccaggaac
     2581 gccacgagcg ccagcacgat cacggcgcgg acgccatggg cgagcatgac cagcatccgc
     2641 cgctccacgc gatccgcgat cgcgccggcg tgcaggccga acagcagcca cggcaggaac
     2701 tgcagcatgg cgccggaggc gaccagcacc ggcgacgagg tcatcgaggc gatcagcagc
     2761 ggagaggcgg cgagggcgat gccgtcgccg atgttgctcg tccacgcggc cgacatcagc
     2821 cagcggaagt cccggccgag ccggcgcggc gcgatgagct ctccgagtgc ggtcactcgc
     2881 tcgatcctag gggcgggtcc ggacatacgc gagaatggga tccatgaccg tctccctgct
     2941 cggcgccacc gagatccgcc gcctcgccgc cgaactcgat gtgaccccca ccaagaagct
     3001 cgggcagaac ttcgtggtcg acgccaacac cgtgcgcaag atcgtgcagg ccgccgatgt
     3061 ccaggcgggg gagcgtgtcg tggagatcgg accgggcctc ggatcgctga ccctcgcgat
     3121 cctcgagacc ggggcgtccg tgaccgccgt cgagatcgat cacaggctcg ccgagcgcct
     3181 tccgcagacg gccgctgagc gcggcgtgcc cgacggtgcc ctcacggtgg tcgacgccga
     3241 cgcgatgcgc gtcaccgagc tgccaggcga cccgagcgtg ctggtcgcca atctcccgta
     3301 caacgtctcc gtgcccgtgc tgctgcactt cctggagacc ttcccgcacc tgcagcgcgg
     3361 cgtggtcatg gtgcaggcgg aggtcgccga gcggctcgcc gcgaagcccg gctcgaagat
     3421 ctacggtgcg cccagcgtga aggcggcctg gtacggcccc tggcggctga ccggcaccgt
     3481 gtcgcggcag gtgttctggc cggtgccgaa cgtcgacagc ctgctggtgg gcttccaccg
     3541 cgaccccgag ccgcgtgggg acgagatcga gcgccggcgc accttccaga tcgtggatgc
     3601 cgcgttcaac cagcgccgca agatgctgcg ccaggcgctg tccggcatct tcggcagctc
     3661 ggcagaggca tccgcggtgc tggaggccgc cggcgtcgcg ccgaccgctc gcggagagga
     3721 cctcacggtc gatgattacc accggatcgc gcgcgtccgg caggacggct agcctggctg
     3781 ggtgaccgat ccccgcttcc gtcccgacgt tcccgagttg caccggccgt acacggcggc
     3841 atccgatcgc tatcagcgat tcgagtaccg ccaggtcggc tcctccggcc tctacctgcc
     3901 gccgatctcg ctcggcctgt ggtggaactt cggcgacaac atccccatgg acgaccagcg
     3961 tgccctgctg cggcacgcgt tcgacagcgg catcacgcac ttcgacctgg cgaacaacta
     4021 cggcccgccg tacggctctg ccgagaagaa cttcggccgc atcttcgcgg aggacctcaa
     4081 gccctaccgc gatgagctga tcatctcgtc gaaggcaggc tacgacatgt ggcccggccc
     4141 gtacggcgac ctgggcagcc gcaagtacat cctcgccagc gcggagcagt cgctcacccg
     4201 catggggctc gactacgtcg acatcttcta ctcgcaccgc gtcgacgcgg tcacgcccgt
     4261 cgaggagacg atcggtgcgc tggacaccct ggtgcgtcag ggcaaggcac tctacgtcgg
     4321 catctcctcg tacagtgcgg agcgcacgga ggcggcgctc gccgtcgcac gcgacctggg
     4381 cacgccgctc gtgatccacc agccgtcgta ctcgatcctg aaccgctggg tagaggacgg
     4441 gctgaccgac acgctccgcg cggagggggt cggcgccatc gcgttcacac cgctcgccca
     4501 gggactgctg accggcaagt acctcgccga cggcacggcc gagcgggcgc agaagcgcgg
     4561 ctcgctgccg gatcggccgc tctccgacga ggggatcgca gtgctgcgcg gcctcaacga
     4621 catcgccgcg gagcgcggtc agagcctcgc gcagatggcg ctgcagtgga ctctgcgcga
     4681 ccccgtggtc gtgtcggcgc tgatcggcgc ctcgcgtccg gagcagctgg acgagaacat
     4741 cgcggccgtc gacggcccgg ccttcacgga tgaggagctc accgccatcg acgccctcgc
     4801 gcacggcatc gacgtcaacc tctggtcgat ctcgtcggat ctgtgacgcg accgtgagct
     4861 tcgcccgaca tccggatgcc gtgcatgtgc gcgcgccggg gaagatcaac gtctacctgg
     4921 gcgtcggtcc gcgtcatgac gacgggtacc acgctctggc gacggtcttc caggcggtgt
     4981 cgctgtacga ggacgtgtac gcctcggcgt ccgacggctt ctcgatcgac gtcgtgggcg
     5041 acgtcgacat ctccggggtg ccgctggacg atcgcaatct cgcactgcgc gctgccaagc
     5101 tgctcgcgca gacggccggc gtgacgcagg gagtgcatct ggacatccgc aagggcgtac
     5161 ccgtcgcggg tggcatggga ggcggctccg cggatgcggc ggcggcgctg gtggcctgtg
     5221 acgccctgtg gggcaccgag ctcggcccgc gcgg
//
LOCUS       DATMSX010000250         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_145674,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000250 DATMSX010000000
VERSION     DATMSX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145674"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1393)
                     /locus_tag="VN041_07615"
     CDS             complement(80..1393)
                     /locus_tag="VN041_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HWU28945.1"
                     /translation="MRTPTSARPPMGWNSWDCYGTTVTEDEVLANARFMSAHLLEYGW
                     DTIVVDIDWSDPTARSHGYNESAPLCMDEHGRLVPDPERFPSSAGGRGFAPLAAEIHA
                     LGLRFGIHTMRGIPRRALAADTPILGTDARASDVADPSNACEWNPDMVGLDHRHPAAQ
                     AYYDSTLAMYAQWGVDFIKADDMLWPYQAADVEAYARAIERSGRPIELSLSPGRDLSL
                     AHLPHLREHATMWRICDDLWDRWEDVEANFARFARWAPHASAHGWPDGDMLPLGRIGL
                     RAERGEPRDDRLTPAERVCLLTLWIIARSPLMIGGDLPSSDPETTALFRNADVLAVLR
                     DSSGGRELFREGDTVIWGADGPGGIRFAAVFNLAGVAREIVLDTRSLALPEGASAVTE
                     LWTGRTVRTEPVEVQSDAARGVAPGSRALRLDIPAHGAALVRCEA"
     gene            complement(1438..>1839)
                     /locus_tag="VN041_07620"
     CDS             complement(1438..>1839)
                     /locus_tag="VN041_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU28946.1"
                     /translation="SLAAHGITPDPRLVRSTPFTLEGGRAAMGELLAEFPEVDGVFCS
                     TDLIAIGAVRALADAGRRVPQDVKVVGFDDVPLASFLTPSLTTVAPDHAAMADAAVTM
                     LIDRIEGRRTADDYRTVVGPVRLIARESTAD"
BASE COUNT          254 a          624 c          706 g          255 t
ORIGIN      
        1 gtcaccgccc gggcgggcat cgacgtattc cgggccggtc cgggtcagcg tgcgctcgtg
       61 ccgccggtgt cgctggcgct cacgcctcgc agcggaccag cgccgccccg tgcgcgggga
      121 tgtccagtcg cagagcacga ctgccgggtg cgacgccgcg ggcggcatcc gactgcacct
      181 cgacgggctc ggtgcgcacg gtgcgtcccg tccacagctc ggtgacggcg gacgcaccct
      241 ccggcagcgc gagggagcgg gtgtcgagga cgatctcgcg tgcgacgccc gccaggttga
      301 acacggcggc gaagcggatg ccgccgggac cgtccgcgcc ccagatcacc gtgtcaccct
      361 cgcggaacag ctcccgccct cctgaggagt cgcgcagcac ggcgaggacg tcggcgttgc
      421 ggaacagcgc ggtggtctcc ggatcgctgg agggcaggtc gccgccgatc atcaacggcg
      481 agcgcgcgat gatccacagc gtcagcaggc agacgcgctc ggcgggggtg agacgatcgt
      541 cacgcggttc cccgcgttcg gcgcggaggc cgatcctgcc cagcggcagc atgtcgccgt
      601 ccggccagcc gtgcgcggat gcatgcggcg cccagcgcgc gaagcgggcg aaattggcct
      661 cgacgtcctc ccaccggtcc cagaggtcgt cgcagatgcg ccacatggtg gcgtgctcgc
      721 gcaggtgcgg aagatgggcg agcgacaggt cacgaccggg cgacaggctc agctcgatcg
      781 gccgcccgct gcgctcgatg gcgcgtgcgt acgcctcgac gtcggccgcc tgatacggcc
      841 agagcatgtc gtcggccttg atgaagtcca cgccccactg cgcgtacatc gcgagggtgc
      901 tgtcgtagta cgcctgcgcg gcgggatgcc ggtggtcgag accgaccatg tcgggattcc
      961 actcgcacgc gttcgacgga tcggcgacgt cggacgccct ggcatccgtg ccgaggatcg
     1021 gtgtgtcggc ggcgagcgcc cggcgcggga tgccgcgcat cgtgtggatg ccgaaccgca
     1081 gcccgagcgc gtggatctcc gcggcgagcg gggcgaaccc gcgtccaccc gcggagctgg
     1141 ggaaccgctc cggatcgggc acgagacgcc cgtgctcgtc catgcacagc ggtgcgctct
     1201 cgttgtagcc gtggctgcgc gcggtggggt ccgaccagtc gatgtcgacg acgatcgtgt
     1261 cccagccgta ctcgagcagg tgggccgaca tgaaccgcgc gttggcgagc acctcgtcct
     1321 ccgtcaccgt cgtgccgtag cagtcccagc tgttccagcc catcggcggg cgggcggagg
     1381 tcggtgtgcg catgggcgtc gggctccttc ggctgggcgg atccggtggt ggcgggttca
     1441 gtccgcggtg gattcgcgtg cgatcaggcg caccggcccg accaccgtgc ggtagtcatc
     1501 ggcggtgcga cggccttcga tgcggtcgat cagcatcgtg acggcggcgt ccgccatggc
     1561 ggcatggtcg ggggcgacgg tcgtcaggga cggggtgagg aaggatgcga gcggcacgtc
     1621 gtcgaagccg accaccttga cgtcctgcgg cacgcggcgc ccggcgtcgg ccagggcccg
     1681 cacggcgccg atggcgatca gatcggtgga gcagaacacg ccgtcgacct ccgggaactc
     1741 cgcgaggagc tcgcccatcg cggcgcggcc gccctcgagc gtgaagggcg tggagcgcac
     1801 caggcgcggg tcgggagtga tgccgtgggc ggcgagaga
//
LOCUS       DATMSX010000251         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13173,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000251 DATMSX010000000
VERSION     DATMSX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13173"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VN041_07625"
     CDS             complement(<1..906)
                     /locus_tag="VN041_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28947.1"
                     /translation="MTPKITAARVVVPEDAPRTVWMLERGEGPTASIISDIAHGGIKT
                     WRRVLEQMMNGSTFRGNRATAAGHSREDALLDEAAEHLHTVAPNRALWASLKNDLHRA
                     TPDGIGANSDGSVVVVEVKSHEHGYEDKGIPRDHMEQMQWQQHVLGAVSALYGSEIRD
                     EDDQPPADGATWTDVPRDDEMIAFLVYRADLFIAWRDAGCPDVDVDLPDDVAAALDAW
                     APLKRELDTAVKAEKKAADALKKAIAKSMPHAERFGAVGIREAGGFQVSVSETTGIDE
                     DAFAADSLPLYRGWVADIARAKERAE"
     gene            complement(903..1217)
                     /locus_tag="VN041_07630"
     CDS             complement(903..1217)
                     /locus_tag="VN041_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28948.1"
                     /translation="MTARIDHVGEALACEEVGLSILREQVLGIAPHMAAVIQFDLARL
                     HAQLALVEQQRIANLIAVAEWNDRALREGARRTDGSEVDYDAERARVDAIFVMVREGL
                     GL"
     gene            complement(1214..1363)
                     /locus_tag="VN041_07635"
     CDS             complement(1214..1363)
                     /locus_tag="VN041_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28949.1"
                     /translation="MSSGYVHNIPITPNPATDEDRAAARRMVARNVPDAAERAQVEAM
                     LGLES"
     gene            complement(1360..1716)
                     /locus_tag="VN041_07640"
     CDS             complement(1360..1716)
                     /locus_tag="VN041_07640"
                     /inference="COORDINATES: protein motif:HMM:NF040315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6085 family protein"
                     /protein_id="HWU28950.1"
                     /translation="MSQLDMITALVSEGIQKNLRANRKYIAASARRDIADDSASVALA
                     VLIDMTGGHHHIVKSDGDTFTLQHPLVERLTGDLLDCDLHQHIAAGEPIEKGRYRVLM
                     NDDPTDVDLIPMKEFE"
     gene            complement(1713..2057)
                     /locus_tag="VN041_07645"
     CDS             complement(1713..2057)
                     /locus_tag="VN041_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28951.1"
                     /translation="MSAPVILPAGDTARARKTDPVTSHAAADSITPEGREASEHEVLA
                     ILADADTPITAEAIASRHLNRAGWGETPHHYTDSRLRTALKQLADDGLVIRDGEGRTK
                     SGRRAATWRAAS"
     gene            complement(2054..2374)
                     /locus_tag="VN041_07650"
     CDS             complement(2054..2374)
                     /locus_tag="VN041_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28952.1"
                     /translation="METRHGRATLGGRRRLLDDIDEAVQTAEEIAGRLYALEPIEVVA
                     MTPVSASAHVRLVGVPTIDPEPRPVAGMHCPCGARGTLYEGEDRDAWDRWVESHDGCS
                     EMVA"
     gene            complement(2463..2702)
                     /locus_tag="VN041_07655"
     CDS             complement(2463..2702)
                     /locus_tag="VN041_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28953.1"
                     /translation="MITLRWALLAGMVACAVAVAVTGRWNGVALLQIGFLLAYFATYQ
                     NVRAAVARFIWGMDPEPGLSRLDELDGLGRHNHQK"
     gene            complement(2699..2893)
                     /locus_tag="VN041_07660"
     CDS             complement(2699..2893)
                     /locus_tag="VN041_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28954.1"
                     /translation="MMKTKEQALRDLGRVVAEEAAIVSKLTVREAAERVYQPGGLSMD
                     ELMSRIADAGICRVESGMAA"
     gene            complement(2890..3150)
                     /locus_tag="VN041_07665"
     CDS             complement(2890..3150)
                     /locus_tag="VN041_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU28955.1"
                     /translation="MATERISERATRIVRGLLGKKGTTQEWLSNESGIPMRTLARRLH
                     NTNPSPMTLDELAAVAEALGTDLVSILVAAREESASRVEQVA"
     gene            3207..3620
                     /locus_tag="VN041_07670"
     CDS             3207..3620
                     /locus_tag="VN041_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU28956.1"
                     /translation="MPTGAQPLPSRLSQEVGRILGEAVAERSATQADIAIAAGMSPAQ
                     LSRALSGKKVFTIDQLDSVCSVLDVDLIDVITRADIASRDRIRPVLAGELIQGRFNVP
                     DTEDTDDDFETPDLETLDFAAKRGTRKADAAPWAE"
     gene            3630..4082
                     /locus_tag="VN041_07675"
     CDS             3630..4082
                     /locus_tag="VN041_07675"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWU28957.1"
                     /translation="MERATAAANQLGYAVMVIALPGDVLACTVPALKRIFVDCRLTED
                     EQRAHLWHEVGHVFHGHACDKHARTAQGRANERQADRFAARKLIDPAKYAALEAVNPD
                     QHHLADELSVPVEYIHVYEQDCLTRVRGTVYTHAREGVGQWAHRAEVA"
     gene            4082..5497
                     /locus_tag="VN041_07680"
     CDS             4082..5497
                     /locus_tag="VN041_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWU28958.1"
                     /translation="MAPTARLRPVGAPAPRAIIYLRQSHYREESISLELQELACRDYC
                     DRMGYTVTAVEADPGISGRTWKRPAVQRVMAAIEARDADVVILWKWSRLSRSRKDWAL
                     AIDRADVAGGKIESATEPIDTATASGRFARGVMTEYAAFQSEQIGEQWEEVRQRRLRM
                     GLPASGRLPFGWVWADGGIVPHETQAHLVVEMYQRYLSGDGAAAIAMWMNEQGIPAPN
                     GGLWRRARPLTVLDSPIHAGLIPYRGATYPGAHEPIIDAATWAAYQRERTQRGAMNAK
                     PRSYQHLLSNLMYCECGARMHGKGSVTGGKLYRGYICSQTLNGHSQRAYVSALTVEPV
                     IASWLMDFDPDVDTTSDGAANRARLDAVIRERNALEIEVVNLTRQHTRNIIPEAAYVR
                     ALADITTEIDRLRADELRLTPTRAANPVQVRSLQQMWHGMSIPAQNKGLRQVIDRIST
                     HADGRSFTVRTVWGDSSEVHA"
     gene            complement(5451..5642)
                     /locus_tag="VN041_07685"
     CDS             complement(5451..5642)
                     /locus_tag="VN041_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HWU28959.1"
                     /translation="MTNKKKNDLPVLTRRRAAILFQDDSGAATAEYAITTMAAVAFAG
                     LLVVIRRALRTSRPTPSAQ"
     gene            complement(5699..>5940)
                     /locus_tag="VN041_07690"
     CDS             complement(5699..>5940)
                     /locus_tag="VN041_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HWU28960.1"
                     /translation="AATDAVLELSRSAGVPAGTLLRASAAQQRHEARVQGRIRAARLS
                     SSLLLPLGACTLPAFLLLGVAPLMLSVLGSTPLPI"
BASE COUNT          911 a         2117 c         1955 g          957 t
ORIGIN      
        1 ctccgcgcgc tccttggcgc gcgcgatgtc ggccacccag ccgcggtaca gcggcagcga
       61 gtcggcagcg aacgcgtcct cgtcgatgcc cgtggtctcg gacacgctca cctggaagcc
      121 gccggcctcg cggatgccga ccgcgccgaa ccgttcggca tgcggcatgc tcttcgcgat
      181 cgccttcttc agtgcgtcgg cggccttctt ctccgccttc accgccgtgt cgagctcgcg
      241 cttcaacggg gcccacgcgt cgagcgcggc ggcgacgtcg tcgggcaggt cgacgtcgac
      301 atcggggcat ccggcatccc gccacgcgat gaacaggtca gcgcggtaga cgaggaacgc
      361 gatcatctcg tcgtcgcggg gaacgtcggt ccacgtggcg ccgtcagcgg ggggctggtc
      421 gtcctcgtcg cggatctccg acccgtacag agccgacacc gcgccgagca cgtgctgctg
      481 ccactgcatc tgctccatgt ggtcgcgcgg gatgcccttg tcctcatagc cgtgctcgtg
      541 ggacttgacc tcgaccacca cgacagaacc gtccgagttc gcgccgatgc cgtccggggt
      601 tgcccggtgc aggtcgttct tgagcgacgc ccagagggcc cggttcgggg ccacggtgtg
      661 caggtgctcg gcggcttcgt cgaggagggc gtcctcgcgg gagtggccgg cggccgtggc
      721 gcggttgccg cggaacgtgg acccgttcat catctgctcg agcacgcgcc gccacgtctt
      781 gatgccgccg tgcgcgatgt ccgagatgat cgatgccgtc ggtccctcgc cgcgctcgag
      841 catccacacc gtgcgcggtg cgtcctccgg gaccacgacc cgggccgcgg tgatcttcgg
      901 agtcacagtc ccagcccctc tcgcaccatc acgaagatcg cgtcgacgcg cgcgcgttca
      961 gcgtcgtagt cgacctctga gccatctgtc ctccgcgcgc cctctcggag ggcacggtcg
     1021 ttccactccg ccactgcgat caggttcgcg atgcgctgct gctcgacgag cgccagctgg
     1081 gcgtgcagtc gagccaggtc gaactggatc accgctgcca tgtgcggcgc gatcccgagc
     1141 acctgctcac gcaggatcga caggccgact tcttcgcacg ccagcgcctc gccgacgtgg
     1201 tcgatgcggg cggtcacgat tccagcccca gcatcgcctc gacctgcgcg cgctcggcgg
     1261 catccgggac gttccgggcg accatgcggc gggccgcggc gcggtcctcg tccgtggccg
     1321 ggttcggcgt gatcgggatg ttgtgcacgt atccgctgct cattcgaatt ccttcatggg
     1381 gatcaggtcg acgtcggtcg ggtcgtcgtt catcaggacc cggtagcggc ccttctcgat
     1441 cggttcgccg gcggcgatgt gctggtgcag gtcgcagtcg aggaggtcgc cggtgaggcg
     1501 ctcgacgagc gggtgctgca gggtgaacgt gtcgccgtcg gacttcacga tgtggtggtg
     1561 gccgccggtc atgtcgatca gcacggccag ggcgacggat gctgagtcgt cggcgatgtc
     1621 gcgccgcgcg gaggcggcga tgtacttccg gttcgcgcgc aggttcttct ggatgccctc
     1681 cgacaccagc gccgtgatca tgtccagctg gctcacgatg cggcccgcca ggttgcggcg
     1741 cggcggccgg acttcgtgcg gccctcaccg tcacggatca cgagcccgtc gtcggcgagc
     1801 tgcttcagcg cggtccgcag gcgggagtcc gtgtagtggt gaggggtctc gccccacccg
     1861 gcgcggttca ggtgccggga cgcgatcgcc tccgcggtga tcggggtgtc cgcgtcggcc
     1921 aggatcgcga gcacctcgtg ctcggatgcc tcccgaccct caggggtgat cgagtcggcc
     1981 gcggcgtgcg aggtgaccgg gtccgtcttc cgtgccctcg cggtgtcacc cgcgggcagg
     2041 atcaccgggg cgctcacgcg accatctccg agcatccgtc gtgcgactcg acccaccggt
     2101 cccacgcgtc acgatcctcg ccctcgtaga gcgtgccgcg tgcgccgcac gggcagtgca
     2161 tgcccgcgac cggacgaggc tcagggtcga tcgtcgggac cccgacgagg cgtacgtgcg
     2221 ccgatgcgga gacgggcgtc atggccacga cctcgatggg ttctagcgcg tacaggcgac
     2281 cggcgatctc ctcggccgtc tgcaccgctt cgtcgatgtc gtcgagcagg cgacgtcggc
     2341 cgccgagagt cgcgcgaccg tgacgcgttt ccacggccaa gaacgtagga ttgtcggtga
     2401 tggacatgtt cggatgtcct ttctcttgat ggccggtccc gctgcaacgg ggccggctct
     2461 cttcacttct ggtggttgtg gcggccgagg ccgtcgagct cgtcgaggcg tgacagaccc
     2521 ggctcggggt ccatgcccca gatgaagcgc gccaccgcgg cgcgcacgtt ctggtaggtc
     2581 gcgaagtacg cgagcaagaa gccgatctgc agcagggcga cgccgttcca gcgacccgtg
     2641 acggccacgg cgacagcgca cgcgaccatc ccggcgagca gggcccagcg gagcgtgatc
     2701 atgccgccat tcccgactcg acccggcaga tgccggcgtc cgcgatgcgg gacatcagct
     2761 catccatcga caggccaccc ggctgataca cgcgctctgc ggcctcacgg accgtcagct
     2821 tcgagacgat cgccgcctct tcagcgacga ctcgaccgag gtccctgagc gcctgctcct
     2881 tcgtcttcat cacgccacct gctccacgcg cgatgcgctc tcctcacgcg ccgccacgag
     2941 gatgctcacc aggtcagtgc cgagcgcttc cgccactgcc gcgagttcgt cgagcgtcat
     3001 cggcgagggg ttcgtgttgt ggaggcgtcg tgcgagtgtc cgcataggga tgccgctctc
     3061 gttcgagagc cactcctgcg tggtcccctt cttgccgagg agaccgcgga cgatgcgcgt
     3121 cgcccgttcg ctgatccgtt ccgttgccat atggcaatga tgcgctagct attgccaaat
     3181 ggcaagggga ctgccaaaat tggcacatgc ctacaggagc acaaccgctg cccagccgcc
     3241 tcagccagga ggttggccgt atcctcggcg aggccgtcgc agagcgctca gccactcagg
     3301 ccgatatcgc catcgcggcg gggatgtcgc ccgcacagct gtcccgcgca ctctcaggca
     3361 agaaggtctt cacaatcgac cagctcgatt ccgtgtgctc cgtgctcgat gtcgacctca
     3421 tcgacgtcat cacgcgtgcc gacatcgcat cgcgggaccg aatccggcca gtcctcgcgg
     3481 gcgaactgat ccaaggtcgg ttcaatgtcc ccgacaccga ggacactgac gatgacttcg
     3541 agacgcccga cctggaaacg ctcgacttcg ccgccaagcg cggcacccgc aaggccgacg
     3601 ccgcgccgtg ggcggaatga gggggaccga tggaacgggc aacagcagca gctaaccaac
     3661 tgggctacgc cgtgatggtg atcgcgctgc ccggcgacgt gctcgcctgc accgtcccgg
     3721 cactcaagcg catattcgtc gactgccgac tcaccgaaga tgagcagcgc gcgcacctct
     3781 ggcacgaggt cggccacgtc ttccacggcc acgcgtgcga caagcacgcg cgcaccgcgc
     3841 agggtcgcgc caacgagcgc caggcggatc gcttcgcagc acgcaagctc atcgacccgg
     3901 cgaagtacgc cgcgctcgag gccgtgaacc ctgaccagca tcacctcgcc gacgagctga
     3961 gcgtgcccgt cgagtacatc cacgtctacg aacaggactg cctcacccgc gtgcgcggca
     4021 ccgtgtacac gcacgcccgc gagggcgtcg ggcagtgggc gcaccgcgcc gaggttgcgt
     4081 gatggccccc accgcacggc tgcgccccgt cggcgcgccc gcgccgcgcg cgatcatcta
     4141 cctgcgacag tcccactacc gcgaagagtc catcagcctc gagctgcagg agctcgcgtg
     4201 ccgcgactac tgcgaccgca tgggctacac ggtcaccgcc gtagaagccg atcccggcat
     4261 ttccggccgc acgtggaagc gccccgccgt gcagcgcgtc atggctgcga tcgaggcacg
     4321 cgacgccgac gtcgtcatcc tgtggaagtg gtctcgactc tcccgttcgc ggaaggattg
     4381 ggccctcgcg atcgaccgag ccgacgtcgc cggtggcaag atcgaatccg ccacagagcc
     4441 tatcgatacc gccaccgcgt ctggccgctt cgcgcgtggt gtcatgaccg agtacgcggc
     4501 gttccagtcc gagcagatcg gcgagcagtg ggaggaggtc cggcagcgtc gcctgcgcat
     4561 gggcctccct gcatccggcc gcctgccctt cgggtgggtt tgggccgacg gcggcatcgt
     4621 gcctcacgag acgcaggctc acctcgtcgt cgagatgtac cagcgctacc tctccggcga
     4681 tggcgccgcg gcgatcgcca tgtggatgaa cgaacagggg atccccgctc cgaacggcgg
     4741 cctgtggcgc cgggctcgcc ccctcaccgt cctcgactca cccatccacg ccggcctgat
     4801 cccctaccgc ggtgccacct acccgggcgc gcacgagccg atcatcgacg ccgcgacgtg
     4861 ggcggcctac cagcgcgaac gtacccaacg aggagccatg aatgccaagc cccgcagcta
     4921 tcaacacctg ctctcgaacc tcatgtactg cgaatgcggc gcccggatgc atggcaaggg
     4981 cagcgtcact ggcgggaagc tctaccgcgg atacatctgc agtcagacgc tgaacggcca
     5041 cagccagcgc gcctatgtct cggcactcac cgtcgagccg gtgatcgcct catggctgat
     5101 ggacttcgac cccgacgtcg acaccacatc cgacggagcc gccaaccgtg cgcgactcga
     5161 cgccgtcatc cgagagcgca acgccctcga gatcgaggtc gtcaacctca cccgacagca
     5221 cacccgcaac atcatccccg aagccgccta cgtgcgcgct ctcgcggata tcacgaccga
     5281 gatcgaccgg cttcgcgccg atgagctgcg cctcactccc acccgcgccg ccaaccccgt
     5341 gcaggtgcgg agtctgcagc agatgtggca tggcatgtcg atccccgcac agaacaaggg
     5401 cctgcgccag gtgatcgacc gaatctctac gcacgccgac ggccgctcgt tcactgtgcg
     5461 gacggtgtgg ggcgactcgt ccgaagtgca cgcctaatca cgaccagcag acccgcgaac
     5521 gcgacggcgg ccatcgtcgt gatggcgtac tccgcggtgg cggcgcccga atcgtcctgg
     5581 aacaggatcg ccgcccgccg acgggtcagg acgggcagat cgttcttctt cttgttcgtc
     5641 atgtcattcc ttctctcttc atctctttct gtggtggtgc gttctctcgg tggcgacgtc
     5701 agatcggcag tggcgtggag cccagcacgc tgagcatgag cggggcgacg ccgagcagca
     5761 ggaaggcagg cagcgtgcac gcgcccagtg gcagcagcag ggacgacgac agccgcgcgg
     5821 cacggatgcg gccctgcacc ctcgcctcgt ggcgctgctg cgccgccgac gcccgcagca
     5881 gcgttcccgc agggacgccg gcgctgcggg acagctccag cacggcgtcg gtcgcggcgc
//
LOCUS       DATMSX010000252         6050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_89136,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000252 DATMSX010000000
VERSION     DATMSX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89136"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..2097
                     /locus_tag="VN041_07695"
     CDS             370..2097
                     /locus_tag="VN041_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28961.1"
                     /translation="MLGKLLVRYLSRYWWLLLLVVVLQFASVMASLNLPQINADIINK
                     GVARADIGYIWARGGFMLLVSLGQIVASVLATFFAARMAMAAGRDIRADVFARVSGFS
                     EREVSQFGAGSLITRNTNDVQQVQMLAMMGATMFVTAPLLAIGGIIYAIQTDIGLSWL
                     IAVSVPLLLIIAVLIIGRMVPLFRANQRKIDNVNSIMREQLTGVRVVRAFVRERIEER
                     RFREVNTDLMVLGRNIGSLFVLMFPLFMLVLNVTIVAVIWFGGVEINAGHAEVGTIFA
                     FMQYIGQIMMGIIMSSFMAMMIPRAAVSAERVGEVLDAEASMSRPANGVKEFPAPGAV
                     AFDDVEFTYPGAESPVLSGISFRAEPGETVAIIGSTGSGKTTLVSLIPRLFDVTGGAV
                     SVGGVDVRDADLDSLWKSIGLVPQRPFLFTGTVASNLRYGREDATDEDLWKALEIAQG
                     RDFVEDMPDGLDSHISQGGTNVSGGQRQRLAIARAIVHQPRILVFDDSFSALDLTTDA
                     RLRQALWRELPDVTKIVVAQRVSTITDADRIVVLEDGKVVGVGTHDELLAANETYKEI
                     VVSQLGVDA"
     gene            2133..4313
                     /locus_tag="VN041_07700"
     CDS             2133..4313
                     /locus_tag="VN041_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28962.1"
                     /translation="MVAARAASAPVVEMTEAEREEAEQAEKARQHGGSMFDGPAPGKA
                     DHFGPSFRRMIGLLKPSAAWFALVSLFGAIGVVLSVAAPKILGEATNIVYEGFISMTL
                     GEGKTAFPKGTTQDQVVEVLRQAKQDTFADMIAAMAPFKVGDGVNFESLRWIIIAVMA
                     IYIVSALLSWLQGYVINVIMVRTMWRLREDVEAKINRLPLSYFDKVQRGELISRVTND
                     IDNITQTMQQSLSGALTSVLTVVGVLVMMFSISWQLALVALVSLPLMAVIFGVIGPRS
                     QKAFGIQWRKVGRLNARVEEAFSGHALVKVFGREQDALDSFQVENEELFQASFKAQFL
                     SGVIMPAMTFVGNLQYVGIAVLGGLMVANGGLRLGDVQAFIQYSQQFSQPLSELGGMA
                     AVVQSGTASAERVFDFLDAEEQEPDDEDAPTVDGGRGVIEFDHVAFSYSEDRPLITDL
                     SFRVEPGQTVAIVGPTGAGKTTLVNLIMRFYELNSGRILLDGQNIAEMARDDLRRRTG
                     MVLQDPWLFGGTIRENIRYGNESATDDEIVAAAAATYVDRFVHSLPDGYDTVLDEDAS
                     NVSAGERQLITIARAFVAQPSILILDEATSAVDTRTELLLQHAMAALRQGRTSFVIAH
                     RLSTIRDADLILVMEHGDIVEQGTHDELIAAQGAYWRLYQSQFEQAASDIDAEDALTG
                     STPVVVTGDAEDAAQEAVAEAADAPQLAEAVREAESAASESDRT"
     gene            complement(4500..>6050)
                     /locus_tag="VN041_07705"
     CDS             complement(4500..>6050)
                     /locus_tag="VN041_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HWU28963.1"
                     /translation="PADDSASPAIAGLFDAPAKADAPELFDEPAPVTAEEPSPLFLSD
                     IENALAAEADTTEVSEPQVEAQPAQVTAWADERHPITALTWVDPLKVGATPSAEPETA
                     PALFAGVRLAPTGRRLRLILPLAIIGALCASYVGGTLLAPLDNVAPAVQNAPVEIAAA
                     PAATVTWPATGSAAVAVLGMNPVASTSDRDQIASLSKVASVMMVLDKLPLKVGEQGPS
                     YAFTRADQREFLRYRRSNQSSLDVPVGGSLTEYQMLQGVLLGSANNYIDRLAREIWGS
                     EAAFADASAKWLRDNGIEGITLENPSGFSKSNVATPEAMLRLSELAMRNPVFAEIVAT
                     RSAEIPGVGTVTNTNKMLDDPGVIGIKTGTLDHWNLLTAKEVKVGDTTIRLFTSVLGQ
                     DSNKDRLAVTRELLAGMEKSLADQPVSVPKGTVVGKVTTEWGSEVNLVTDSDVRVVLW
                     NGATATATADLALGSKTTAGAKAGTLSAKGPVNTAETTVSLTKEVTAPSIWWRLTHPL
                     ELFGIDKQ"
BASE COUNT          927 a         2046 c         2067 g         1010 t
ORIGIN      
        1 ggtgcgacgg tcacgacgcc gtcggcggtc agcgcatcgg catccggggc cagcaccacg
       61 acaggcactc cctcgctcgc cagggcctcg gcctgccgca cgaacgcggt ctgcgcgccg
      121 cccaggtact tcagcgaata atcgcagacc agcaggacag cagaaccggt tcgcatgcct
      181 gcgagtctat tcgcggcacc ctgaccgatt tctcgatcgg atccaggaaa acgggccccg
      241 tgggagcggt cccggcgccc gtttcgccct cagcgaaggt catccctgag ggggatacga
      301 atgtccgcgg ttcgtggcac tctgtcatgt ggccctctcc gggccccgat cccaaaagga
      361 agtccgcctg tgctgggaaa actcctcgtt cgctacctct ccagatactg gtggctgctg
      421 ctcctcgtcg tggtgctgca gttcgccagc gtgatggcat ctctcaacct gccgcagatc
      481 aacgccgaca tcatcaacaa gggcgtggcc agggccgaca tcggctacat ctgggcgcgc
      541 ggcggcttca tgctgctggt ctcgctcggc cagatcgtcg cctcggtcct cgcgaccttc
      601 ttcgccgccc gcatggcgat ggcggccggt cgtgacatcc gcgccgacgt gttcgcgcgc
      661 gtgagcggct tctccgagcg cgaggtctcg cagttcggcg ccggctcgct gatcacccgc
      721 aacaccaacg acgtgcagca ggtgcagatg ctggcgatga tgggcgcgac gatgttcgtc
      781 accgccccgc tgctcgccat cggcggcatc atctacgcca tccagaccga catcggcctc
      841 agctggctga tcgccgtctc ggtcccgctg ctgctcatca tcgccgtgct gatcatcggc
      901 cgcatggtgc cgctcttccg tgccaaccag cgcaagatcg acaacgtcaa cagcatcatg
      961 cgcgagcagc tgaccggcgt gcgggtcgtg cgcgcgttcg tgcgcgagcg catcgaggag
     1021 cggcgcttcc gcgaggtcaa caccgacctg atggtgctcg gacgcaacat cggttcgctg
     1081 ttcgtgctga tgttcccgct gttcatgctg gtgctcaacg tgacgatcgt cgcagtgatc
     1141 tggttcggcg gcgtggagat caacgccggc cacgccgagg tcggcacgat cttcgccttc
     1201 atgcagtaca tcggccagat catgatgggc atcatcatgt ccagcttcat ggccatgatg
     1261 atcccgcgcg ctgcggtctc cgccgagcgc gtcggcgagg tgctcgacgc cgaggcgtcg
     1321 atgagccgcc cggcgaacgg cgtgaaggag ttcccggctc ccggggcggt cgccttcgac
     1381 gacgtcgaat tcacctaccc cggcgccgag tcgcccgtgc tcagcggcat cagcttccgt
     1441 gcggagccgg gcgagaccgt cgcgatcatc ggctccacgg gctccggcaa gaccacgctg
     1501 gtgtccctca tcccccgcct gttcgacgtc accggcggcg ccgtgagcgt gggcggcgtc
     1561 gatgtgcgcg acgccgacct cgactcgctg tggaagagca tcggtctggt gccgcagcgt
     1621 ccgttcctgt tcaccggcac ggtcgcgtcc aacctccgct acggccggga ggacgccacc
     1681 gacgaggatc tctggaaggc gctcgagatc gcgcaggggc gcgacttcgt cgaggacatg
     1741 cccgacgggc tggactcgca catctcgcag ggtggcacga acgtctcggg cggtcagcgt
     1801 cagcgactcg cgatcgcccg cgccatcgtg caccagccgc gcatcctcgt cttcgacgac
     1861 tcgttctctg cactcgacct gaccacggat gcccgcctga ggcaggcgct gtggcgcgag
     1921 ctgccggacg tgaccaagat cgtcgtcgcg cagcgcgtgt cgacgatcac ggatgccgac
     1981 cgcatcgtcg tgctcgagga cggcaaggtc gtcggcgtgg gcacgcacga tgagctgctc
     2041 gccgccaacg agacctacaa ggagatcgtc gtgtctcagc tgggggtgga cgcatgagcg
     2101 gggaggacaa gaagatgagc cgccgcgagc gcatggtcgc cgcacgcgcc gcatccgcgc
     2161 ccgtggtcga gatgaccgag gccgagcgcg aagaggccga gcaggccgag aaggcccgcc
     2221 agcacggcgg cagcatgttc gacgggcccg cgcccggcaa ggccgatcac ttcggcccga
     2281 gcttccggcg catgatcggg ctgctcaagc cgtcggcggc gtggttcgcg ctggtgtcgc
     2341 tgttcggcgc catcggcgtg gtgctgtccg tcgccgcgcc caagatcctc ggtgaggcga
     2401 cgaacatcgt ctacgagggc ttcatctcca tgacgctcgg cgagggcaag acggccttcc
     2461 cgaagggcac cacccaggac caggtcgtcg aggtgctgcg ccaggcgaag caggacacct
     2521 tcgccgacat gatcgcggcg atggctccgt tcaaggtcgg cgacggcgtg aacttcgagt
     2581 cactgcgctg gatcatcatc gcggtcatgg cgatctacat cgtctcggcg ctgctgagct
     2641 ggctgcaggg ctacgtgatc aacgtgatca tggtgcgcac catgtggagg ctgcgcgagg
     2701 acgtcgaggc gaagatcaac cgcctgcccc tgtcgtactt cgacaaggtg cagcgcggtg
     2761 agctgatctc gcgggtcacc aacgacatcg acaacatcac gcagaccatg cagcagtcgc
     2821 tgtccggggc gctcacctcg gtgctcacgg tcgtgggcgt gctggtcatg atgttctcga
     2881 tctcgtggca gctcgcactg gtcgcgctgg tgtcgctgcc gctgatggcc gtcatcttcg
     2941 gcgtcatcgg accgcggtcg cagaaggcgt tcggcatcca gtggcgcaag gtcggccgcc
     3001 tgaacgcccg tgtcgaggag gccttctcgg gccacgccct cgtcaaggtg ttcggccgcg
     3061 agcaggacgc cctcgacagc ttccaggtcg agaacgagga gctgttccag gcgagcttca
     3121 aggcgcagtt cctgtcgggt gtgatcatgc ccgccatgac gttcgtcgga aacctgcagt
     3181 acgtcggcat cgcggtgctc ggcggtctga tggtcgccaa cggcggtctg cggctcggcg
     3241 acgtgcaggc gttcatccag tactcgcagc agttcagcca gccgctcagc gagctgggtg
     3301 gcatggcggc cgttgtgcag tcgggcacgg cgtcggccga gcgcgtcttc gacttcctgg
     3361 atgccgagga gcaggagccg gatgacgagg acgccccgac cgtcgacggc ggccgcggtg
     3421 tgatcgagtt cgaccacgtg gccttctcgt acagcgagga ccgtccgctg atcaccgacc
     3481 tgtcgttccg ggtggagccg ggccagacgg tcgcgatcgt cggcccgacc ggtgccggca
     3541 agacgacgct ggtcaacctg atcatgcggt tctacgagct gaactcgggc cggatcctgc
     3601 tcgacgggca gaacatcgcc gagatggcgc gtgacgacct gcgacgccgt accggcatgg
     3661 tgctgcagga cccgtggctg ttcggcggca cgatccgcga gaacatccgg tacggcaacg
     3721 agtccgcgac cgacgacgag atcgtcgccg ccgcggcggc gacctatgtc gaccggttcg
     3781 tgcactcgct gccggacggc tacgacaccg tgctcgacga ggacgcgtcg aacgtctcgg
     3841 cgggtgagcg ccagctgatc acgatcgccc gcgcgttcgt cgcccagccg tcgatcctca
     3901 tcctcgacga ggccaccagc gcggtcgaca cccgtaccga gctgctgctg cagcacgcca
     3961 tggcggcgct gcgtcaggga cgcacctcgt tcgtgatcgc gcaccgtctc tcgacgatcc
     4021 gcgacgccga cctcatcctc gtgatggagc acggcgacat cgtcgagcag ggcactcacg
     4081 acgagctgat cgccgcccag ggcgcctact ggcgcctgta ccagtcgcag ttcgagcagg
     4141 cggcatccga catcgacgcc gaggacgccc tcaccggcag cactccggtg gtcgtgacgg
     4201 gcgacgccga ggatgccgcg caggaagcgg tcgccgaggc ggcggacgcc ccgcagctgg
     4261 ccgaggccgt gcgcgaggcg gagtcagcgg catccgagtc cgaccggacc tgagccgccg
     4321 caccagaccg gcacatgcag aacgcccgcc ccttccgggg cgggcgttct cgtgtctggg
     4381 cccagttcga ccggacagat gtcggcccga agtacggatg ctggtcgaga agcggcctgc
     4441 gggccgacat ccgtgatctg aaccgtcgat gcgggccagg ggctgaattc gggcgggacc
     4501 tactgcttgt cgatgccgaa cagctcgagc gggtgcgtca gccgccacca gatgctcggg
     4561 gctgtgacct ccttggtgag cgacaccgtg gtctctgccg tgttcaccgg acccttggcg
     4621 ctcagtgtgc cggccttcgc accggccgtt gtcttcgaac cgagtgcgag atccgcggtg
     4681 gcggtggcgg tcgcgccgtt ccacagcacc acgcgcacgt cggagtccgt gacgaggttc
     4741 acttcgctgc cccactcggt cgtgaccttc ccgacgaccg tgcccttcgg gaccgacacc
     4801 ggctgatcgg cgagcgactt ctccatgccg gccagcagct cccgggtcac ggcgagccga
     4861 tccttgttgc tgtcctgccc gagcaccgag gtgaacaggc ggatggtggt gtcgcccacc
     4921 ttcacctctt tggccgtcag caggttccag tggtcgagcg tgccggtctt gatgccgatc
     4981 acgccagggt cgtcgagcat cttgttggtg ttggtcacgg tgccgacgcc cgggatctcg
     5041 gcgctgcggg tggcgacgat ctctgcgaag acggggttgc gcatcgccag ctcgctgagc
     5101 cgcagcatcg cctcgggcgt cgcgacgttc gacttgctga acccggacgg gttctccagc
     5161 gtgatgccct cgatgccgtt gtcgcgcagc cacttcgccg aggcatcggc gaaggcggcc
     5221 tccgagcccc agatctccct ggcgagccgg tcgatgtagt tgttcgccga tcccagcagc
     5281 acaccctgga gcatctggta ctcggtcagg ctgccgccga cgggcacatc cagagaggac
     5341 tggttcgagc gccggtatct caggaactcg cgctgatccg ccctggtgaa cgcatagctg
     5401 ggtccctgct cgcccacctt gagaggcagc ttgtcgagca ccatcatcac cgaggcgacc
     5461 ttgctgaggc tcgcgatctg gtcgcggtcg gaggtggagg ccaccgggtt catgcccagc
     5521 acggccaccg cggcgctgcc cgtggcgggc caggtcacgg tcgcggccgg tgccgcggcg
     5581 atctcgacgg gcgcgttctg caccgccggt gcgacgttgt cgagcggagc gagcagcgtg
     5641 ccgccgacgt agctcgcgca cagagcaccg atgatcgcga gagggaggat cagccgcagc
     5701 cgtcgtcctg tgggagcgag gcggactccg gcgaacagcg cgggggcggt ctccggctcg
     5761 gccgacggcg tcgccccgac cttcagcgga tcgacccagg tcagcgcggt gatcggatgc
     5821 cgttcatcgg cccacgccgt cacctgggcg ggctgcgcct cgacctgggg ctcggagacc
     5881 tcggtggtgt cggcctccgc ggccagcgcg ttctcgatgt cgctcaggaa gaggggggaa
     5941 ggctcctcag cggtcacggg agcgggctcg tcgaacagct ccggggcatc ggccttcgcc
     6001 ggcgcgtcga acaggccggc gatcgcgggg gaggcggagt catcggcggg
//
LOCUS       DATMSX010000253         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_174408,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000253 DATMSX010000000
VERSION     DATMSX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174408"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1572)
                     /locus_tag="VN041_07710"
     rRNA            complement(46..1572)
                     /locus_tag="VN041_07710"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          390 a          472 c          369 g          484 t
ORIGIN      
        1 taaattaaaa gaaggaaaca acagctcaag gttaatgagc tctaaaaagg aggtattcca
       61 gccgcacctt ccgatacggc taccttgtta cgacttagcc ccaattaccg attttaccct
      121 aggcggttcc ttgcggttgc cgacttcagg tccccccggc tttcatggct tgacgggcgg
      181 tgtgtacaag gtccgggaac gtattcaccg tatcattgct gatatacgat tactagcgat
      241 tccagcttca tgaggtcgag ttgcagacct caatccgaac tgagatagag tttttgagat
      301 tagcagcttg ttaccaagta gcagcccttt gtctctacca ttgtagcacg tgtgtagccc
      361 tgggcataaa ggccatgatg acttgacatc atcccctcct tcctcgcgtc ttacgacggc
      421 agtttcacta gagttcccac cttgacgtgc tggcaactag tgataggggt tgcgctcgtt
      481 gcgggactta acccaacacc tcacggcacg agctgacgac agccatgcag caccttacaa
      541 tctgtgtatt gctacaaaga cacctttcag cgccggtcag actgcattct agcccaggta
      601 aggttcctcg cgtatcatcg aattaaacca catgctccac cgcttgtgcg gacccccgtc
      661 aattcctttg agtttcaacc ttgcggtcgt acttcccagg tggattactt aatgctttcg
      721 ctcagacaca cattgtgtat cacgtatgtc gagtaatcat cgtttagggc gtggactacc
      781 agggtatcta atcctgtttg atccccacgc tttcgtgcct cagcgtcaat atttgtgtag
      841 ccagctgcct tcgcaattgg tgttctatgt catatctatg catttcaccg ctacatgaca
      901 tattccgcta acctccacaa cattcaagac aaatagtatc catggcagtt tctgggttaa
      961 gcccagagat ttcaccacgg acttaagtgt ccgcctacgc accctttaaa cccagtgaat
     1021 ccggataacg cttgcaccct ccgtattacc gcggctgctg gcacggagtt agccggtgct
     1081 tattcacctg gtaccgtcaa gctccctaga aaggaagtgt ttcgtcccag ataaaagaag
     1141 tttacaaccc agagggcctt catcctccac gcggcatggc tggttcagac ttccgtccat
     1201 tgaccaatat tccttactgc tgcctcccgt aggagtcggg cccgtgtctc agtgcccgtg
     1261 tgactggtcg cgctctcacg ccagttactg atcgtcggct tggtgagccg ttacctcacc
     1321 aactacctaa tcagacgcac gcccatcttt aagtgataaa tctttaaatg ctgactgatg
     1381 ccagtccaca ttcttatggg gtattaatcc aaatttctct gggctattcc ccgcttaaag
     1441 gaaggttgcg tacgtgttcc gcacccgttt gccggtcgcc acccagtatt gctacctgtg
     1501 ctgcccctcg acttgcatgt attaggcctg ccgctagcgt tcatcctgag ccaggatcaa
     1561 actctccatt gtaaagaagt tttgatactg actaatttct ctcgaaatcg gtcggatcgt
     1621 tttttaatta tgttagcttt aacattacct gtgtttgatt caaactatca ctaatttgaa
     1681 tcgtgctgtt gtttccattc tttcaaagaa cttac
//
LOCUS       DATMSX010000254         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_108155,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000254 DATMSX010000000
VERSION     DATMSX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108155"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VN041_07715"
     CDS             <1..943
                     /locus_tag="VN041_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU28964.1"
                     /translation="VILSAAALQASGTATAERIGAGRWTLALPASYVAGLQVLARSLL
                     AGTDPILLDGKLTPDALIEAADRGSRYISVVPAQLAALVDAADDPRVLAALRAHDAIL
                     VGGQALSDVIRMRAAELRIPYVHTYGSSETSGGCVYDGIPLDGVSVRLIDDELRLSGP
                     MLADGYLGDTALTARVFETDEHGTRWYRTGDRGFILDGEVEVHGRLDNVIVSGGINIS
                     LDRVEQVVRSVPGLEQAVVLGMPDERWGESSIIFADWGAGDGGRMLDEARARVAEKIG
                     RHARPVSLQGGEIPLLASGKPDRELLRRMIHDVPPRD"
     gene            1013..>1523
                     /locus_tag="VN041_07720"
     CDS             1013..>1523
                     /locus_tag="VN041_07720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU28965.1"
                     /translation="MAVYTHGHHDSVLRSHNTRTVANSAAYLEPLLSADAHLLDVGAG
                     PGTITVDFASRVGRVTATEINPEALSLSQALAAERGATNIDFSVEDVHALSFADDSFD
                     IVHAHQVLQHVGDPVQALREMRRVTRPGGTVAVRDADYAGFIWFPLLPELDEWLRLYR
                     EAARANGGEP"
BASE COUNT          210 a          554 c          548 g          211 t
ORIGIN      
        1 cgtgatcctc agcgcggccg cgctgcaggc gagcggcacg gcgaccgccg agcggatcgg
       61 cgccggacga tggacgctcg cgctcccggc cagctacgtc gcgggtctgc aggtgctggc
      121 gcgctccctg ctcgcgggca ccgatccgat cctcctcgac ggcaagctga ccccggatgc
      181 cctgatcgag gcggccgatc gggggagccg gtacatctcc gtcgtccccg ctcagctggc
      241 ggcgctggtc gacgcggccg acgatccgcg agtgctcgcc gcgctgcgcg cccacgatgc
      301 gatcctggtc ggcgggcagg cgctgtccga cgtcatccgg atgcgcgccg ccgagctgcg
      361 gatcccgtac gtgcacacgt acggatcgag cgagaccagc ggcggctgcg tctacgacgg
      421 catcccactg gacggcgtct ccgtgcggct gatcgacgac gagctgcggc tcagcgggcc
      481 catgctcgcc gacggctacc tgggcgacac cgcgctcacg gcgcgcgtgt tcgagaccga
      541 cgagcacggc acccgctggt accgcacggg tgaccgcggc ttcatcctcg acggcgaggt
      601 cgaggtgcac ggccggctcg acaacgtgat cgtctccggc ggcatcaaca tctccctcga
      661 ccgggtggag caggtcgtgc gcagcgtgcc cgggctcgag caggcggtcg tgctggggat
      721 gcccgatgag cgctgggggg agtcgtcgat catcttcgcc gactggggcg ccggtgacgg
      781 gggacggatg ctggacgagg cgcgtgcgcg cgtcgccgag aagatcggcc ggcatgcccg
      841 cccggtttcg ctgcagggcg gggagatccc gcttctggca tcgggcaagc ccgatcgcga
      901 gctgctgcgg cggatgatcc acgacgtccc gccccgggac tgagcgttcg cgcgcgacgc
      961 ctcggtgaaa cgcggtgcga cgggttcccg cacgccgcct aggctgaccg acatggccgt
     1021 ctacacccac gggcaccacg actcggtgct gcgctcgcac aacacccgca ccgtcgcgaa
     1081 ctccgcggcc tacctcgagc cgctcctgag cgcggacgcg cacctgctcg acgtcggggc
     1141 ggggcccggc accatcaccg tcgacttcgc gagccgcgtg ggtcgtgtca ccgccaccga
     1201 gatcaacccc gaggcgctca gcctgtccca ggcgctcgcg gccgaacgcg gcgcgacgaa
     1261 catcgacttc tcggtcgagg acgtgcatgc gctgagcttc gccgacgaca gcttcgacat
     1321 cgtccatgcg caccaggtgc tgcagcacgt cggcgatccc gtgcaggcgc tgcgcgagat
     1381 gcggcgcgtc accaggcccg gtggcacggt cgcggtgcgc gacgccgact acgccggctt
     1441 catctggttc cccctgctgc ccgagctcga cgagtggctg aggctgtacc gcgaggccgc
     1501 gcgggccaac ggcggcgagc cgg
//
LOCUS       DATMSX010000255        10670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13211,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000255 DATMSX010000000
VERSION     DATMSX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13211"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VN041_07725"
     CDS             complement(<1..499)
                     /locus_tag="VN041_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWU28966.1"
                     /translation="MTRTWTPGSHDEAVTAIARTPVLLVALDFDGTASPLVADPMSAR
                     ALPEIADAVARLAVLPDTEVAYVSGRSLVHLREIAEHSDDSPVILAGSHGAQYWFPGE
                     GEADAAESAQERDLRESLFDELEPLLTEYPGVELERKTFGVGIHGRPADPAVERAAFA
                     AVDEVM"
     gene            complement(496..1917)
                     /locus_tag="VN041_07730"
     CDS             complement(496..1917)
                     /locus_tag="VN041_07730"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWU28967.1"
                     /translation="MAAAEFVVVANRLPVDRIETADGEEIWRTSPGGLVAALEPMMRS
                     VDGAWVGWAGMPDVELAPFDADGIRLIPVALSEADVQEYYEGFSNDTIWPLYHDVIAP
                     PQYHREWWEAYVSVNLRFAEAASAAVAKGGTVWVHDYQLQLVPEMVRNLRPDVTIGYF
                     HHIPFPSHGLYAQLPWRDQVLRGLLGADVIGFQRSQDAANFLAAVKRRLGFETKASAI
                     TLPDGRRSHARAYPISIDTTPYRELAQRADIRARAVEIREGLGNPKHILLGVDRLDYT
                     KGIRHRIKAYGELLDQGRISVEDVTFIQVASPSRERVDAYVHLRDEVELAIGRINGDH
                     DTLGHTAIRYLHQGFPREEMVAMYLAADVMLVTALRDGMNLVAKEYVATRADNRGVLV
                     LSEFTGAADELRQAVRVNPHDIEGVKDAIVSAIEMPRTEQSRRMRSLRKRVLEHDVTA
                     WSQSFLKALEHASHHRHQLEDPA"
     gene            complement(1965..3185)
                     /locus_tag="VN041_07735"
     CDS             complement(1965..3185)
                     /locus_tag="VN041_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU28968.1"
                     /translation="MTQIDSSPSLLRLAGLPYFLIAFIARLPFAMMVVGVLTIVVSAR
                     DSLSLGGLTSAAVGIGTALIGPFLGAAADRYGQRPVLVAAAVLNAAMLAAFAAVVYSP
                     LAQGFVLLAAFAIGATAPQVSPMSRSRLVTIIDERMPQPRRARTTSATMSYESAADET
                     VFVFGPFLVGALASLIAPWAPMVMASALTLMFVGAFALHPTARHVSASRAADGTAPSA
                     VSELFRPTLLVVVLGILGVGLFFGAMLTALTSFMADRGVAEQAGLLYGVMGIGSAILA
                     LGVAWLPPRFTLHLRWLVFGGILLAGSVLVITASDVPTMTVALAILGIGVGPTLVTLY
                     SLGAARSPLGRSATVMTMLGAGVVVGQAAAAAITGELAEGSGTRAALIMPLIAAAIVV
                     ATGIANRLMTPARR"
     gene            3322..3600
                     /locus_tag="VN041_07740"
     CDS             3322..3600
                     /locus_tag="VN041_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28969.1"
                     /translation="MRYLFSTGLIGAITAGMSLLRGTREAPITWRAVLAWISWGITLA
                     LAIGAAVDMRRDEHGLSVAYDSPLAPKQTKRAKKQLKAIEKAAKGTKH"
     gene            complement(3704..4882)
                     /locus_tag="VN041_07745"
     CDS             complement(3704..4882)
                     /locus_tag="VN041_07745"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWU28970.1"
                     /translation="MSDIVIVAAARTPQGRLKGQLAPFTAPQLGSFAIRGALEQAAIS
                     PTDVDAVVMGQVLAAGSGQNAARQAAIGAGIGWDVPASSVNKVCLSGLTAIIDAARMI
                     RTGDATTVVAGGMESMTRAPHLLMGSRDGWTYGTVEVLDHMAYDGLTDAYDKESMGAS
                     TERQNPRYEMTRELQDAVAALSHQRAAAGQADGAFDAEIVPVTVPQRKGDPLVLTQDE
                     GIRPETTVETLAGLRPAFAEGGTITAGNSSQISDGAAAVVVTTRENAEAKGWPVLAVV
                     GASGQTAGPDNSLQAQPARAIEKALGKQGITTDDLDLVEINEAFGAVVARSQQELGLS
                     SDIVNVHGGGIAIGHPIGASGTRLVVHLVHELAKRGSGTAVAALCGGGGQGEALILMR
                     "
     gene            complement(4879..5532)
                     /locus_tag="VN041_07750"
     CDS             complement(4879..5532)
                     /locus_tag="VN041_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HWU28971.1"
                     /translation="MALTRNEMAARAAQELTDGSYVNLGIGLPTLVPNYVPEGVTVVL
                     QSENGILGVGPYPTEENVDPDLINAGKETVTLLPGAAFFDSATSFGMIRGGKIDAAIL
                     GAMQVSATGDLANWMIPGKMVKGPGGAMDLVHGAGRVIILMEHVAKDGSTKIVNDCTL
                     PLTGRGVVHRIITDLAVIDVTPDGLVLVETAPGVTAQEVIDATEPPLTVSENLKENS"
     gene            complement(5534..6313)
                     /locus_tag="VN041_07755"
     CDS             complement(5534..6313)
                     /locus_tag="VN041_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HWU28972.1"
                     /translation="MIDKQFASAAEAVADIPDGASLAVGGFGLSGNPMLLIEALLAQG
                     TKDLSVVSNNCGVDDWGLGVLLGARRIRKMTSSYVGENKEFERQFLEGDLELELTPQG
                     TLAEKLRAGGSGIAAFFTQTGVGTQVAEGGLPRRYNADGTIAVASPVKETRSFDFNGV
                     EREFVLEESITTDFALVHALKGDRHGNLVFNKAARNFNPLAAMAGRICIAQVEELVEP
                     GELDPDGIHLPGVFVHRIVEVGTDIEKRIERHTVAAGQGGD"
     gene            6418..7212
                     /locus_tag="VN041_07760"
     CDS             6418..7212
                     /locus_tag="VN041_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWU28973.1"
                     /translation="MESTAPGRRSVPGAQAIARASALLRLVSASTDGASLQQLATDSS
                     LSRSTAHRLLTALKVEGLVDQDPTTSCWTAGPELYLMGTAAASRYDITEISRDIVRSL
                     AVKTEESAFLSTRRADETVCLIREEGSFPIRSFVLSEGIRFPLGVASAGIAILSFLPD
                     HDVDAYLERHPELEGRWGAAHGPEPLRKRLAETKARGYAVNPGLIVEGSWGIGAAVFD
                     REGRAEWALSLTGAEFRFGPDRLPQLGRTLLAHAHQLSSRIAGARR"
     gene            complement(7213..8118)
                     /locus_tag="VN041_07765"
     CDS             complement(7213..8118)
                     /locus_tag="VN041_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU28974.1"
                     /translation="MERSLMPHTYRTIDVPVAGGALRVAIWEPVGETVATALLIHGVT
                     SSHLAWPFVVERMSGVRIIAPDLRGRGMSSSVEGPAGMAAHADDMAAVLDAVGVASTT
                     VVGHSMGAFVAVAFAHRHPDRVRRLVLVDGGFPLDLPAGVDPEQAVQVVLGPVAERLS
                     MRWSSVQEYTDRFWRNHPALAEDWSDELERYIAYDLVPEGDAFRAATTYRSMADDTRD
                     MNVGTLLPEALAALSKPALFITVPRGLQNETPGLYPPAHLEKVLAQFPQVRHVRLDDL
                     NHYTVVMSERGADALAPLVLAELGS"
     gene            complement(8103..8855)
                     /locus_tag="VN041_07770"
     CDS             complement(8103..8855)
                     /locus_tag="VN041_07770"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HWU28975.1"
                     /translation="MTEQDLAGRRAVVTGGASGIGLACAKEFAGRGAHVIIADLNAEA
                     AAAAADEVGGEAWVVDLSDTAALDDLELQADILVNNAGIQRVSPIAEFDPDAFRLIQR
                     IMLEAPFLLIRAVLPGMYERGWGRIINVSSVHGLRASAFKSAYVAAKHGLEGLSKVTA
                     LEGGPHGVTSNCINPGYVRTPLVEKQIADQARLHGIPEGEVVERIMLTESAIKRLVEA
                     AEVASLAGWLASDAAGMVTGASYTMDGGWSAR"
     gene            complement(8852..9553)
                     /locus_tag="VN041_07775"
     CDS             complement(8852..9553)
                     /locus_tag="VN041_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU28976.1"
                     /translation="MITPTEEPPVSPATGRPLTKRGQLTRRRLLEAAEQVFADLGYHE
                     ASIVKITEHAGVALGTFYLYFDSKQSIFEALVVDLNSRVRHSMSEAMAGAEDRIEAER
                     RGFEGFFRFTAQHPALYRVVREAEFVSPAMLKLHYERIVDGYRAGLAAAQRDGEVASE
                     LDTEVVAWALMGAGELIGMRYLLWETDAEGRPPAEIDPAVLDGMTAFITRALRPDTTA
                     SQTTKASPTTKGAAG"
     gene            complement(9537..>10670)
                     /locus_tag="VN041_07780"
     CDS             complement(9537..>10670)
                     /locus_tag="VN041_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU28977.1"
                     /translation="DAPPAEQAVAARPARDDDPLLVIYTSGSEAAPKGVVLTHENCFW
                     NNLALDRAMPLGADDVVLAILPQYHVAAWNVQPLQAWWRGATVVLERSFDPGRVLQLI
                     PERGVTTMMGVPTQYRMLLQHPNWASADLASLRRVVVGGATIPDDLVTSWAARGLTFT
                     QGYGLTEAGPNVLYLAPELVADHPGAVGRPYPGVDARLVDPATGLELRGEATGELWVR
                     GASVFPGYLDDPDATARARSGEWLRTGDLLHRDADGIHRVVDRLKEIYVSGGENIAPA
                     EIEGVLNRHPLVAASAVVGVPDPVWGERGCAFVVTDGPVSADELRAFTAERLAAFKLP
                     AHIVLLDELPRSTIDKVARGRLRRMAQDLLADPPLKEEHDHAH"
BASE COUNT         1564 a         3741 c         3767 g         1598 t
ORIGIN      
        1 ccatcacctc gtccaccgcc gcgaacgcgg cgcgctccac cgccggatcc gcaggacggc
       61 cgtggatgcc gactccgaag gtcttccgct ccagctccac gcccgggtac tccgtgagca
      121 gcggctcgag ctcgtcgaac agcgactcgc gcaggtcgcg ctcctgcgcg ctctcggcgg
      181 catccgcctc gccctccccc gggaaccagt actgcgcgcc gtgggagccc gcgaggatca
      241 ccggtgagtc gtcggaatgc tccgcgatct cgcgcaggtg cacgaggctc cgcccggaga
      301 cgtaggccac ctcggtgtcg ggcagcaccg ccagtcgcgc cacggcatcc gcgatctcgg
      361 gcagggcgcg cgcgctcatc gggtcggcga ccaggggtga cgccgtgccg tcgaagtcga
      421 gcgcgaccag cagcacgggc gtgcgggcga tcgcggtgac ggcctcgtcg tgactgcccg
      481 gtgtccaggt gcgggtcatg cggggtcctc cagctggtga cggtggtggg acgcgtgctc
      541 gagcgccttc aggaacgact gagaccaggc ggtgacgtcg tgctcgagca cgcgcttgcg
      601 cagcgatcgc atccggcggc tctgctcggt gcgcggcatc tcgatcgccg agacgatggc
      661 atccttcacg ccctcgatgt cgtgcgggtt cacccgcacc gcctgccgaa gttcgtcggc
      721 cgcgccggtg aactcgctga gcacgagcac gccgcgattg tcggcgcgag tggcgacgta
      781 ctccttcgcg acgaggttca tgccgtcgcg cagcgccgtc accagcatca cgtcggcggc
      841 caggtacatc gccaccatct cctcgcgagg gaatccctgg tgcagatacc ggatcgccgt
      901 gtgcccgagc gtgtcgtggt cgccgttgat gcggccgatg gcgagctcca cctcgtcgcg
      961 caggtgcacg taggcgtcga ctcgctcgcg gctggggctc gccacctgga tgaaggtgac
     1021 gtcctccacg gagatcctgc cctggtcgag cagctccccg taggccttga tgcggtgccg
     1081 gatgcctttc gtgtagtcca gccggtccac gcccagcagg atgtgcttcg gattgccgag
     1141 cccctcacgg atctccaccg cgcgggcacg gatgtcggcg cgctgcgcga gctcgcggta
     1201 cggcgtcgtg tcgatcgaga tggggtacgc cctcgcgtgg ctgcggcggc cgtcgggcag
     1261 ggtgatggcg ctggccttcg tctcgaaccc taggcggcgc ttgaccgcgg cgaggaagtt
     1321 cgcggcgtcc tgggaacgct ggaagccgat cacgtcggcg ccgagcaggc cgcgcagcac
     1381 ctggtcccgc cacggcagtt gcgcatagag cccgtgggag gggaacggga tgtggtggaa
     1441 gtagccgatg gtcacgtccg gacgcaggtt gcgcaccatc tcgggcacca gctgcagctg
     1501 gtagtcgtgc acccagacgg tgccgccctt cgcgaccgct gcggacgcgg cctcggcgaa
     1561 gcgcagattc accgagacgt acgcctccca ccactcgcgg tgatactgcg gcggggcgat
     1621 cacgtcgtgg tacagcggcc agatcgtgtc gttgctgaag ccctcgtagt actcctggac
     1681 gtcggcctcg ctgagcgcga cggggatcag gcggatgccg tcggcgtcga acggggcgag
     1741 ctcgacatcc ggcatccccg cccagcccac ccaggcaccg tccacgctgc gcatcatggg
     1801 ctcgagtgcc gccacgagcc cgccgggcga agtgcgccag atctcctcgc catcggctgt
     1861 ctcgatccgg tcgaccggca gtcggttggc gacgacgacg aactctgcgg ctgccatggg
     1921 gactcctctg ctcggttcct ccaggctacc gatcgcggcg cggctcagcg ccgggctggc
     1981 gtcatgagcc gatttgcgat accggtcgcg acgacgatgg cggcggcgat caggggcatg
     2041 atgagtgccg ctcgggtgcc cgagccctcg gccagttctc cggtgatcgc cgctgcggcc
     2101 gcctggccga ccacgacccc tgcgcccagc atggtcatca cggtggccga gcgtcccagc
     2161 gggctgcgtg cggcccccag gctgtacagc gtgacgagcg tcggcccgac gccgatgccg
     2221 aggatcgcga gggcgacggt catggttggg acatcggatg ccgtgatcac cagcaccgat
     2281 ccggccagca ggatgccgcc gaacaccagc cagcgcaggt gcagcgtgaa ccgcggcggc
     2341 agccaggcga cgccgagagc caggatcgcc gagccgatcc ccatcacgcc gtacagcagt
     2401 ccggcctgct cggcgacgcc gcgatcggcc atgaacgacg tgagggcggt gagcatcgcc
     2461 ccgaagaaca ggcccacgcc gaggatgccg agcaccacca cgagcagcgt gggacggaag
     2521 agctccgaca cggcggacgg cgcggtgccg tccgccgcgc gggacgcgga gacgtgccgc
     2581 gcggtggggt gcagggcgaa tgcgccgacg aacatcaggg tgagggcgga tgccatcacc
     2641 atcggcgccc agggggcgat cagcgaggcg agcgcgccga cgaggaacgg accgaacacg
     2701 aacacggtct cgtcggccgc ggactcgtag gacatggtcg ccgaggtcgt acgggcgcgc
     2761 cgtggctgcg gcatccgctc gtcgatgatg gtgaccagcc gcgatcgcga catgggcgac
     2821 acctgcggcg cggtggcgcc gatcgcgaag gcggcgagca gcacgaagcc ctgggcgagc
     2881 gggctgtaca cgacggcggc gaacgcggcg agcatcgccg cgttgagcac cgcagcggcg
     2941 accagcaccg ggcgctgacc gtagcggtcg gcggcagctc ccaggaacgg gccgatcagc
     3001 gccgtgccga tcccgacggc ggcggaggtg aggccaccca gggacaggga gtcgcgggcg
     3061 ctgaccacga tggtcagcac gccgacgacc atcatggcga acggcagtcg cgcgatgaac
     3121 gcgatgagaa agtacggaag gccggcgagg cgcagcaggc tcggcgagga atcgatctgt
     3181 gtcatggctc tcgggcgccg catcgcgacg ccgggtcggg tgccgccgct gtccgccggg
     3241 gagccggcgg ccggtagata cacagctgta actgctgtca agcctagccg aggagggatg
     3301 ctcgtcggct aacgtgaagc catgcggtat ctcttcagca ctggactcat cggcgccatc
     3361 acggccggaa tgtcactgct gcgcggcacc cgcgaggcgc cgatcacgtg gcgcgccgtg
     3421 ctcgcctgga tcagctgggg catcacgctc gccctcgcga tcggcgccgc cgtcgacatg
     3481 cgccgcgacg agcacggtct ctcagtcgcc tacgactcgc cgttggctcc gaagcagacc
     3541 aagcgggcca agaagcagct caaggcgatc gagaaggccg ccaaaggcac caagcactga
     3601 gcgacgacac cctgtcctct cgtcgacacc ccgggtgtgg acctccacag cccggggtgt
     3661 cgacgatttc acggggtgtc gacggagatg catccgctga gcgtcagcgc atgaggatca
     3721 gcgcctcgcc ctgaccgccg ccaccgcaga gggcggccac ggcagtgccg gacccgcgct
     3781 tggcgagctc gtgcacgagg tgcacgacca gtcgtgttcc gctggcgccg atcggatgcc
     3841 cgatcgcgat gccgccgccg tgcacgttca cgatgtcgct ggacagtccc agctcctgct
     3901 gggaccgcgc gaccacggcg ccgaacgcct cgttgatctc gaccaggtcg agatcgtccg
     3961 tcgtgatgcc ctgcttgccc agggccttct cgatcgcccg ggccggctgc gcctgcagcg
     4021 agttgtccgg gcccgcggtc tggccgctgg cgcccacgac ggccagcacg ggccagccct
     4081 tggcctccgc gttctcccgc gtggtgacga cgacggccgc cgcaccgtcg gagatctgcg
     4141 aggagttgcc ggccgtgatg gtgccgccct cggcgaacgc cgggcggagc cccgcgaggg
     4201 tctcgaccgt cgtctcgggg cggatgccct cgtcctgggt gagcacgagc ggatcgccct
     4261 tgcgctgcgg caccgtgacc ggcacgatct cggcatcgaa cgcgccgtcg gcctgccctg
     4321 cggcggcacg ctggtgggac agcgccgcga ccgcatcctg caactcgcgg gtcatctcgt
     4381 agcgcgggtt ctgacgctcg gtggacgctc ccatgctctc cttgtcgtac gcgtcggtca
     4441 ggccgtcgta cgccatgtga tcgagcacct cgaccgtgcc gtaggtccag ccgtcgcggg
     4501 agcccatcag caggtgcggc gcgcgggtca tggattccat gcctccggcg accaccgtgg
     4561 tggcgtcgcc ggtgcggatc atgcgcgcgg cgtcgatgat cgcggtgagc ccggagaggc
     4621 agaccttgtt gaccgagctg gccgggacgt cccagccgat gccggcgccg atcgcggcct
     4681 ggcgtgccgc gttctgcccg gagccggccg cgaggacctg gcccatgacc acggcgtcga
     4741 cgtccgtcgg cgagatggcg gcctgctcga gggcgccccg gatcgcgaac gagccgagct
     4801 gcggcgcggt gaacggtgcg agctgcccct tcagacgtcc ctgcggggtg cgggcagcgg
     4861 cgacgatgac gatgtcgctc atgagttctc cttcaggttc tccgagaccg tcagcggcgg
     4921 ctcggtggcg tcgatgacct cctgcgcggt cacgccgggt gccgtctcca cgagcacgag
     4981 cccgtcaggc gtgacgtcga tgaccgcgag gtcggtgatg atgcggtgca cgacccccct
     5041 gccggtcagc gggagcgtgc agtcgttcac gatcttggtg gagccgtcct tggcgacgtg
     5101 ctccatcagg atgatcacgc ggccggcgcc gtgcaccagg tccatcgcgc cgccggggcc
     5161 cttgaccatc ttgcccggga tcatccagtt ggccaggtct cccgtggcgg agacctgcat
     5221 ggcgccgagg atggcggcgt cgatcttgcc gccgcggatc atgccgaaac tcgtcgccga
     5281 gtcgaagaac gccgcaccgg gcagcagggt gacggtctcc ttgcccgcgt tgatcaggtc
     5341 aggatcgacg ttctcctcgg tcgggtacgg tccgacgccg aggatgccgt tctcggactg
     5401 cagcacgacg gtcacgccct cgggcacgta gttcggaacc agcgtgggca ggccgatgcc
     5461 gaggttcacg tacgagccgt cggtgagctc ctgcgcggcg cgggccgcca tctcgttacg
     5521 cgtgagcgcc atgtcagtcg cctccctgtc cggcggcgac ggtgtgccgt tcgatgcgct
     5581 tctcgatgtc ggtccccact tcgacgatcc ggtgcacgaa gacgcccggc aggtggatgc
     5641 cgtcggggtc gagctcgccg ggctcgacga gctcctcgac ctgagcgatg cagatgcgcc
     5701 cggccatggc ggcgagcggg ttgaagttgc gggcggcctt gttgaagacg aggttcccgt
     5761 gccggtcgcc cttcagcgcg tgcacgagcg cgaagtcggt ggtgatggac tcctccagca
     5821 cgaactcgcg ctcaacgccg ttgaagtcga acgagcgggt ctccttgacg ggagacgcga
     5881 ccgcgatcgt cccgtcggcg ttgtagcggc gcggcagccc gccctcggcg acctgcgtgc
     5941 cgacgccggt ctgcgtgaag aacgcggcga tgcccgagcc tccggcgcgg agcttctcgg
     6001 ccagcgtgcc ctgcggggtg agctccagct ccagatcgcc ctcgaggaac tgccgctcga
     6061 actccttgtt ctcgccgacg taggacgagg tcatcttgcg gatgcgccgt gcgccgagca
     6121 gcacgcccag cccccagtca tccacgccgc agttgttgct gaccacgctg aggtccttcg
     6181 tgccctgcgc gagcagcgcc tcgatgagca gcatcgggtt cccagagaga ccgaatccgc
     6241 cgaccgcgag cgacgctccg tcggggatgt cggcgacggc ctcggccgcc gatgcgaact
     6301 gtttgtcgat cactgaccac tccttcgtga atgtcctcct ccagcatccg cccagcgcga
     6361 cgacggcggc aagccgatgc ttgcgatgtg gtcacgaatc cgggtagcgt ccacattgtg
     6421 gaatcaacgg caccggggag gcgctccgtc cccggagccc aggcgatcgc ccgggcctcc
     6481 gccctgctgc ggctggtgtc ggcgtcgacc gacggcgcat cgctgcagca actggcgacg
     6541 gattcttcgc tcagccgctc gactgcccac cggctgctca ccgcgctgaa ggtcgaggga
     6601 ctggtggatc aggatccgac cacctcatgc tggacggccg gtcctgagct gtacctgatg
     6661 ggcacggccg ccgcgtcgcg ctacgacatc acggagatct cgcgcgacat cgtgcgctcg
     6721 ctggcggtga agacagagga gagcgcgttc ctgtccacgc gacgcgcgga tgagacggtg
     6781 tgcctgatcc gcgaagaggg gtcgttcccg atccgctcct tcgtgctcag cgagggcatc
     6841 cgctttccgc tcggcgtcgc cagcgccggc atcgcgatcc tgtcgttcct acctgaccac
     6901 gatgtggatg catatctcga gcgccacccg gagctggaag ggcgctgggg tgcggcgcac
     6961 ggccccgagc cgctgcgaaa gcggctcgcg gagacgaagg cgcgcgggta cgccgtgaat
     7021 cccgggctga tcgtggaggg ctcatggggc atcggcgccg cggtgttcga tcgcgaggga
     7081 cgcgccgagt gggcgctgag tctcaccggc gccgagttcc gcttcggccc cgaccggctc
     7141 ccccagctcg gccggacgct gctggcgcac gcgcaccagc tctcctcccg gatcgcgggc
     7201 gcccgacggt agtcagctcc ccagttcggc gagcaccagc ggcgccagtg cgtccgcccc
     7261 gcgctcggac atcacgaccg tgtagtggtt gaggtcgtcg agacgcacgt gccgcacctg
     7321 cgggaactgg gccagcacct tctccaggtg cgccggcggg tagagccccg gcgtctcgtt
     7381 ctgcaggccg cgcggcacgg tgatgaacag cgccggcttc gacagcgccg ccagcgcctc
     7441 cgggagcagc gtgccgacgt tcatgtcgcg ggtgtcgtcg gccatcgacc gataggtggt
     7501 cgcagcgcgg aacgcgtcgc cctccgggac gaggtcgtag gcgatgtacc gctccagctc
     7561 gtcgctccag tcctcggcca gcgcgggatg attccgccag aaccggtcgg tgtactcctg
     7621 cacgctcgac cagcgcatcg agaggcgctc ggcgacaggc cccagcacga cctgcaccgc
     7681 ctgctcgggg tcgacgcccg caggcagatc cagcgggaac ccaccgtcga cgagcaccag
     7741 gcgacgcacg cgatccggat gcctgtgcgc gaacgcgacc gcgacgaatg cgcccatcga
     7801 gtgccccacg acggtcgtgg acgccacccc cacggcatcc agcaccgcgg ccatgtcgtc
     7861 ggcgtgcgcg gccatgcccg ccggcccctc gacggagctg ctcatccccc ggccgcgcag
     7921 gtcgggtgcg atgatccgga cgccggacat ccgctcgacg acgaacggcc aggccaggtg
     7981 cgaggacgtg accccgtgga tcagcagcgc cgtcgcgacg gtctccccca ccggttccca
     8041 gatcgcgacg cgcagggcgc cgcccgcgac ggggacgtcg atggtgcggt aggtgtgcgg
     8101 catcagcgag cgctccatcc gccgtccatg gtgtacgacg caccggtcac catgccggcg
     8161 gcgtcggatg ccagccatcc cgcgagggag gcgacctcgg cggcctccac gaggcgcttg
     8221 atcgcactct ccgtgagcat gatccgctcc acgacctcgc cctcggggat gccgtgcagc
     8281 cgcgcctggt cggcgatctg cttctcgacg agcggggtgc gcacgtagcc ggggttgatg
     8341 cagttgctgg tcacgccgtg cgggccgccc tccagcgccg tcaccttcga caggccttcc
     8401 agcccgtgct tggcggcgac gtacgccgac ttgaacgcgc tggcacgcag accgtgcacg
     8461 ctggagacgt tgatgatccg cccccagccg cgctcgtaca tgcccggcag cacggcgcgg
     8521 atgagcagga acggcgcctc gagcatgatc cgctggatga gccggaaggc gtcggggtcg
     8581 aactcggcga tcgggctcac ccgctggatg ccggcgttgt tcaccaggat gtcggcctgc
     8641 agctcgaggt catcgagcgc tgcggtgtcg gagagatcga ccacccacgc ctccccgccg
     8701 acctcgtcgg cggcagccgc cgcggcctcg gcgttcagat cggcgatgat cacgtgcgct
     8761 ccgcggccgg cgaactcctt ggcgcaggcc aggccgatcc cactcgctcc cccggtgacg
     8821 acggcccgcc tgccggcgag gtcctgttcg gtcatcctgc tgcccccttc gtggtcggtg
     8881 aggccttcgt tgtctgtgag gccgtggtgt ccgggcgcag cgcccgggtg atgaatgcgg
     8941 tcatgccgtc gaggacggcg gggtcgatct cggcaggcgg gcgaccctcg gcatccgtct
     9001 cccagagcag gtagcgcatg ccgatcagct cgccggcgcc catcagcgcc cacgcgacca
     9061 cctcggtgtc cagctcggac gcgacctcgc cgtcgcgctg tgcagcagcg aggccggctc
     9121 ggtagccgtc cacgatgcgt tcgtaatgca gcttgagcat ggcgggcgag acgaactccg
     9181 cttcgcggac gacccggtac agcgccggat gctgggcggt gaaccggaag aagccctcga
     9241 agcccctccg ctcagcctcg atgcggtcct cggcgcccgc catcgcctcg gacatggagt
     9301 gccgaacacg actgttcagg tcgacgacca gggcctcgaa gatcgactgc ttgctgtcga
     9361 agtacagata gaaggtgccc agcgcgaccc cggcgtgctc ggtgatcttc acgatcgacg
     9421 cctcgtggta gccgagatcg gcgaacacct gctcagccgc ctccagcagg cggcggcgtg
     9481 tcagctgacc tcgcttggtc agcggccgtc ccgtcgccgg ggagaccggc ggttcctcag
     9541 tgggcgtgat catgttcctc cttcagaggc gggtccgcga gcaggtcctg cgccatccgc
     9601 cgcaaccggc ctctggcgac cttgtcgatg gtcgaacggg gcagttcgtc gagcagcacg
     9661 atgtgcgcgg gcagcttgaa cgcggcgagc cgctccgcgg tgaacgcgcg cagctcgtcc
     9721 gccgacacgg ggccgtcggt caccacgaac gcgcagccgc gttcacccca caccgggtcg
     9781 ggcacgccga cgacggcgga ggcggcgacc agcggatgcc ggttcagcac gccctcgatc
     9841 tcggccgggg cgatgttctc cccgcctgag acgtagatct ccttgagccg gtccaccacc
     9901 cgatggattc cgtcggcgtc gcggtgcagc agatcaccgg tgcggagcca ctccccgctc
     9961 ctggcccgcg cggtcgcatc ggggtcatcg aggtagccgg ggaagacgct cgctccgcgc
    10021 acccacagct ccccggtcgc ctcaccccgc agctcgaggc ccgtcgccgg gtcgaccagc
    10081 cgcgcgtcca cgcccgggta gggcctgccg accgcaccgg ggtgatcggc gacgagctcg
    10141 ggagccaggt acagcacgtt gggtcctgcc tcggtgagtc cgtagccctg ggtgaacgtc
    10201 aggccgcggg cggcccagct cgtgacgagg tcgtcgggga tggtcgcccc gccgaccacc
    10261 actcgacgca gcgatgcgag gtcggcggat gcccagttcg ggtgctgcag cagcatccgg
    10321 tactgggtcg gcacgcccat catggtggtg acgccgcgtt cagggatcag ctgcagaacg
    10381 cgaccagggt cgaaggagcg ctccagcacg acggtcgcgc cgcgccacca cgcctgcagc
    10441 ggctgcacgt tccaggcggc cacgtggtac tgcggcagta tggcgagcac gacgtcgtcg
    10501 gcgccgaggg gcatggcccg gtccagcgcc aggttgttcc agaagcagtt ctcgtgcgtg
    10561 aggacgacgc ccttgggcgc cgcctcgctg ccggaggtgt agatcacgag cagcgggtcg
    10621 tcgtcgcggg ctggtcgcgc ggctacggcc tgctcggcgg gcggcgcgtc
//
LOCUS       DATMSX010000256         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_4095,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000256 DATMSX010000000
VERSION     DATMSX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="galT"
                     /locus_tag="VN041_07785"
     CDS             <1..702
                     /gene="galT"
                     /locus_tag="VN041_07785"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWU28978.1"
                     /translation="VCFSPEPEGSLGTQSVTRARTIIEAWADRTAALSALPGIEQVFP
                     FENRGEAIGVTLPHPHGQIYSYPYITPRTQRLLDSITRTAPDLMTRVLESEQASSRVV
                     LQNEHWTAFVPFAARWPLEVQVMPHRHAADFTELTSAERDALAPLFLRLLRGVDALYE
                     TPTAYIGAWHQAPVNVGRDSIRMRFELTSPRRAADKLKFLAGTEAAMNAWSAEILPED
                     TAARLRDAIDTIAAP"
     gene            734..1921
                     /gene="galK"
                     /locus_tag="VN041_07790"
     CDS             734..1921
                     /gene="galK"
                     /locus_tag="VN041_07790"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HWU28979.1"
                     /translation="MTALTAARDLFASSFGHEPEGVWSAPGRVNLIGEHTDYNEGFVL
                     PFAIQHRTYAAVAPRTDDRIRVTSSFARLTVECALDDLESMFPVEYQGADRDVPEWSA
                     YVLGVAWALRASTGSPAPQRGVDIAIASDVPVGAGLSSSAAIEGAVASALNDLWELGL
                     DPVTLAQVGRKAENEAVGAPTGIMDQMSSMLGRPDAATFIDCRSLEARSIPTGFRDAG
                     LEVLVIDTQVTHAHSTGGYRERRESCERGAQLLGIRSLRDASVSDLPRAAEIMDDVTF
                     RRVRHIVTENQRVLDTVAALTEQGPQAIGDLLVASHASMRDDFEISVAELDTAVNTAL
                     ANGAIGARMTGGGFGGAAIALVSHADLQRVSDAVEATFAYAGFTAPHIFTVSPSEGPR
                     RDA"
     gene            1986..>2863
                     /gene="galE"
                     /locus_tag="VN041_07795"
     CDS             1986..>2863
                     /gene="galE"
                     /locus_tag="VN041_07795"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWU28980.1"
                     /translation="MSWLVTGGAGYIGAHVVRALASAGLTPVVLDDLSSGRPSFVPEG
                     VTLVEGSILDRELIERTLREQNAEGVIHVAGFKYAGVSVQRPLHTYAQNVEGTRVILE
                     AMEAAGVANIVFSSSAAVFGTPDVELVTEDTAKRPASPYGESKLIGEWLLHDQGVATA
                     ASEKPLRHTSLRYFNVVGSADPTVYDVSPHNLFPIVFEALLEGRTPQINGDDYDTPDG
                     TNVRDYVHVGDIAAAHVVAAQRLASGESIEPAYNLGSGDGLSVKQIMDAMVRVTGIDF
                     VPAIGPRRPGDPDRIVA"
BASE COUNT          422 a         1092 c          946 g          403 t
ORIGIN      
        1 gtctgcttca gccccgagcc cgaggggtcg ctgggcaccc agtccgtcac ccgcgcgcgc
       61 accatcatcg aggcgtgggc ggaccgcacc gcggccctgt cggcgctgcc cggcatcgag
      121 caggtgttcc cgttcgagaa ccgcggcgag gccatcggcg tgaccctccc gcatccgcac
      181 ggccagatct actcgtaccc gtacatcacc ccgcgcacgc agcgtctgct cgacagcatc
      241 acgcgcaccg cgcccgacct gatgacgcga gtgctcgagt cggagcaggc ctccagccga
      301 gtcgtgctgc agaacgagca ctggacggcg ttcgtgccct tcgccgcccg ctggccgctc
      361 gaagtgcagg tcatgcccca ccggcatgcc gccgacttca ccgaactgac gtccgcggaa
      421 cgggatgcgc tcgccccgtt gttcctgcgg ctgctgcgcg gcgtcgacgc actgtacgag
      481 accccgactg cgtacatcgg cgcctggcat caggctccgg tgaacgtcgg ccgcgacagc
      541 atccggatgc gattcgagct gaccagcccg cgccgcgccg ccgacaagct gaagttcctg
      601 gccggcaccg aggccgccat gaacgcctgg agcgccgaga tcctccccga ggacacggcg
      661 gcccgcctgc gcgacgcgat cgacaccatc gccgccccct gacctgccgc accactgcat
      721 ccctggagtc cccatgaccg cactcaccgc cgcccgtgac ctgttcgcgt cgtccttcgg
      781 gcacgagccc gagggggtct ggtccgctcc cggacgcgtg aacctcatcg gcgagcacac
      841 cgactacaac gagggcttcg tgctgccgtt cgcgatccag caccgcacgt acgcggcggt
      901 ggcgccgcgc accgacgacc ggatccgggt gacctcgagc ttcgcgcgcc tgaccgtcga
      961 gtgcgcgctc gacgatctgg agagcatgtt ccccgtcgag taccagggcg ccgaccgtga
     1021 cgtcccggaa tggtcggcgt acgtgctcgg cgtcgcgtgg gcactgcgcg cttcgacggg
     1081 ctcaccggcc ccgcagcgcg gcgtggacat cgcgatcgcc tccgacgtgc cggtcggcgc
     1141 gggactgtcc tcctcggcag cgatcgaggg agccgtggcg tccgccctga acgacctctg
     1201 ggagctgggc ctggatccgg tcaccctcgc ccaggtcggt cgcaaggccg agaacgaggc
     1261 cgtcggcgca ccgaccggga tcatggatca gatgtcgtcc atgctcggcc gaccggatgc
     1321 cgcgaccttc atcgactgcc gcagcctgga ggccaggtcc attccgaccg gcttccgcga
     1381 cgccgggctc gaggtgctcg tgatcgacac gcaggtcacg catgcccact ccaccggcgg
     1441 ctaccgggag cgccgggagt cctgcgaacg gggcgcacag ctgctcggca tccgctccct
     1501 gcgcgacgcg agcgtctccg acctgccgcg cgcggccgag atcatggacg acgtcacgtt
     1561 ccgccgggtg cgccatatcg tcaccgagaa ccagcgcgtg ctcgacacgg tcgccgccct
     1621 caccgagcag gggccgcagg cgatcggcga tctcctggtc gcctcgcacg cgtccatgcg
     1681 cgacgacttc gagatctcgg tcgccgagct cgacacggcg gtgaacacag ccctggcgaa
     1741 cggcgcgatc ggcgcccgga tgaccggggg cggcttcggc ggcgcggcga tcgcgctggt
     1801 ctcgcacgcg gatctgcagc gggtgtcgga tgccgtggag gcgacgttcg cgtatgccgg
     1861 cttcaccgcc ccgcacatct tcacggtctc gccctccgag gggccgcgcc gcgacgcctg
     1921 accccgcccg gcgcaccgcg cctgacacaa tggaacccgc gcgtccgcgc gacggaagga
     1981 gacccatgtc ctggcttgtc accggcggtg ccggctacat cggagcgcac gtggtgcgcg
     2041 cactggcatc cgcgggtctc accccggtcg tgctggacga cctgtccagc gggcgcccgt
     2101 cgttcgtgcc cgagggcgtg acgctggtcg agggcagcat cctcgaccgc gagctcatcg
     2161 agcgcaccct gcgcgagcag aacgcggagg gcgtgatcca cgtcgccggc ttcaagtacg
     2221 ccggtgtctc cgtgcagcgc ccgctgcaca cctacgcgca gaacgtggag ggcacccgcg
     2281 tgatcctcga ggccatggag gccgcgggcg tcgcgaacat cgtgttctcc tccagcgccg
     2341 ccgtcttcgg cacccccgac gtcgagctcg tcaccgagga caccgccaag cggccggcca
     2401 gcccctacgg cgagtcgaag ctcatcggcg agtggctgct gcacgaccag ggcgtcgcga
     2461 cggcggcatc cgagaagccc ctgcggcaca cctcgctgcg ctacttcaac gtggtcggct
     2521 ccgccgaccc gacggtctac gacgtgagcc cgcacaacct gttcccgatc gtgttcgagg
     2581 cgctgctcga gggccgcacc ccgcagatca acggcgacga ctacgacacc cccgacggca
     2641 ccaacgtgcg cgactacgtg cacgtcggcg acatcgcggc cgcgcacgtc gtggccgcac
     2701 agcggctcgc ctccggcgag tcgatcgagc ccgcctacaa cctcggctcg ggtgacgggc
     2761 tgagcgtgaa gcagatcatg gatgccatgg tgcgcgtcac cggcatcgac ttcgtccccg
     2821 cgatcggtcc gcgccgcccc ggcgaccccg accgcatcgt cgc
//
LOCUS       DATMSX010000257         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_89261,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000257 DATMSX010000000
VERSION     DATMSX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89261"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VN041_07800"
     CDS             complement(<1..1369)
                     /locus_tag="VN041_07800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HWU28981.1"
                     /translation="MSVPGSGRPRSMSAFAAVDLGATSGRVMIGRVHDDRVELEPVSR
                     FPNGPVERADGLHWDFDALFENIVEGLAEAVRREPGIRSIGIDSWAVDYGLLRDGELL
                     AEPFHYRDNRTARGVEQVHAVVPFEELYRRNGLQFLPFNTLYQYRVEEQLPQADAALL
                     IPDLIAFLLTGRRVAERTNASTTGLLDVRTSEWDVELAERLGIPVGVLPDLVDPGTVI
                     GTLRPELARRIGAELRVVAVGSHDTASAVVATPLSSPQSAYISCGTWGLVGLELAEPV
                     VTDAARAANFTNELGVDGRIRFLHNVTGLWLLSETVRAWEAEDGAPIDLPSLLAQAAD
                     ADGETPLFDANEPSLSAPGDMPARIAALLGPDAPVSRPAFARSIVDSIAAAFADAVRT
                     ASALSSREIDSIHLVGGGSLNRLLCQATADRSGLPVLAGPVEATALGNVLIQARATQD
                     PPTSLE"
     gene            complement(1366..>2127)
                     /locus_tag="VN041_07805"
     CDS             complement(1366..>2127)
                     /locus_tag="VN041_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU28982.1"
                     /translation="GAASGIGKAIATRLAAEGACVVVADLDLEKAQAAAAELGNTDVA
                     IGVAANVADADAVQAALDEAVLAFGGVDLVVNNAGLSLSKPLLETTEKDWDLQHDVMA
                     KGSFLVSKAAAKVLIEQKLGGDIIYISSKNSVFAGPNNIAYSATKADQAHQVRLLAVE
                     LGEYGVRVNGINPDGVVRGSGIFASGWGANRAATYGVAEEDLGQFYANRTILKREVVP
                     ENVADAVYVLTGPELSRTTGLHIPVDSGVAAAFLR"
BASE COUNT          313 a          743 c          757 g          314 t
ORIGIN      
        1 gctccagcga cgtcggcgga tcctgcgtgg cgcgcgcctg gatcagtacg ttcccgagcg
       61 cggtcgcctc gaccgggccg gcgagcaccg gaagtccgga gcggtcggcg gtcgcctggc
      121 agagcaggcg gttgagggac ccaccgccga ccaggtggat gctgtcgatc tcgcggctgg
      181 agagagcgga tgccgttcgc acggcgtcgg cgaacgccgc cgcgatcgag tccacgatgc
      241 tgcgcgcgaa ggccggtcgt gagaccggcg cgtccgggcc gagcagtgcc gcgatacgtg
      301 ccggcatgtc accgggtgcg ctgagcgacg gctcgttcgc gtcgaacagg ggcgtctcgc
      361 cgtcggcatc cgccgcctgc gcgagcagcg acggcaggtc gatcggcgcg ccatcctctg
      421 cctcccacgc gcgcacggtc tcgctgagca gccagaggcc cgtcacgttg tgcaggaagc
      481 ggatgcggcc gtccacgccg agctcgttgg tgaagttcgc tgcgcgcgcg gcatccgtca
      541 ccaccggctc tgccagttcc agccctacca gcccccaggt tccgcaggag atgtacgccg
      601 actgcggcga ggacagcggg gtggcgacga cggcggatgc cgtgtcgtgc gagccgaccg
      661 ctaccacccg cagttccgca ccgatccggc gggcgagctc tggccgcagc gtgccgatca
      721 cggttcccgg gtcgacgagg tcgggaagga cgccgaccgg gatgccgagc cgttcggcga
      781 gctcgacgtc ccactccgac gtgcgcacgt ccagcaggcc ggtcgtcgac gcgttcgtgc
      841 gctccgcgac ccgccgccct gtgagcagga acgcgatcag gtcggggatc agcagtgcgg
      901 catccgcctg ggggagctgc tcctcgaccc ggtactggta cagggtgttg aacggcagga
      961 actgcaggcc gttgcggcga tacagctcct cgaacgggac gacggcgtgg acctgctcca
     1021 caccgcgcgc ggtgcgattg tcgcggtagt ggaacggctc cgcgagcagt tcgccgtcgc
     1081 gcagcagccc gtagtccacg gcccaggaat cgatgccgat gctccggatg cccggttcgc
     1141 ggcgcaccgc ctcggccagg ccctcgacga tgttctcgaa cagggcgtcg aagtcccagt
     1201 gcaggccgtc ggctctctcg accgggccgt tggggaagcg cgagaccggc tcgagctcga
     1261 cccgatcgtc gtgcacgcgt ccgatcatca cgcggccgct cgtggccccg agatcgacag
     1321 cggcgaaggc gctcatcgac cgggggcgcc ccgacccggg aacgctcatc gcaggaaggc
     1381 cgcggcgacg ccggagtcga cggggatgtg cagacccgtg gtgcgcgaca gctcggggcc
     1441 ggtcagcacg tacacggcgt cggcgacgtt ctcgggaacg acctcgcgct tgaggatggt
     1501 gcggttcgcg tagaactggc ccagatcctc ctctgccacg ccgtaggtcg cggcgcggtt
     1561 cgcgccccag cccgaggcga agatgcccga gccgcgcacg acgccgtcgg ggttgatgcc
     1621 gttcacgcgc acgccgtact cgccgagctc caccgccagc agacgcacct ggtgcgcctg
     1681 gtcggccttg gtcgcggagt aggcgatgtt gttcgggccc gcgaacaccg agttcttcga
     1741 ggagatgtag atgatgtcgc caccgagctt ctgctcgatc agcaccttgg ctgcggcctt
     1801 cgacaccagg aaggagccct tcgccatgac gtcgtgctgc aggtcccagt ccttctcggt
     1861 ggtctccagc agcggcttcg acagcgacag accggcgttg ttgaccacca ggtccacgcc
     1921 gccgaacgcc agcacggcct cgtcgagcgc ggcctgcacg gcatccgcat ccgcgacgtt
     1981 cgctgcgacg ccgatcgcga catccgtgtt tccgagttca gcggccgcgg cctgagcctt
     2041 ctcgagatcg aggtcggcga cgacgacgca cgcgccctct gcggcgagac gggtggcgat
     2101 ggccttgccg atgccggatg ccgcgcc
//
LOCUS       DATMSX010000258         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13253,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000258 DATMSX010000000
VERSION     DATMSX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13253"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2027)
                     /locus_tag="VN041_07810"
     CDS             complement(219..2027)
                     /locus_tag="VN041_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28983.1"
                     /translation="MTITGTRDEDRSDLTKEESRAIRQRSLRLLGSLIHPLRLRIVIA
                     AIVLVVSTALQVAGPILIGIALDSALPQLLKNADWMPAIMVTAVYLFAGIVGSALIGF
                     YVVLAARITQAVLLDLRKRIFLHTQRLSLEFHESYTSGRIISRQTSDLDSIRELLDGG
                     LNNLVSGVLFGIFTFIALVIADWQAGVVLLLAGVPLVLLMRWFYRRSQLVYRESRVIS
                     AKVIVQFVETMTGIRAVKSFRKEPRNDVAFRKVAGDYRDINRRSMVLFGTFEPGLMAV
                     SALTITLVVLWGGLRVADGALGVGVLLATVLYVRNFFAPMQEIAMFLNSYQSATAALE
                     KVSGVLEEVPTVPDPQKPIDLWEARGAIRFDDVTFGYNGDKTILPSFSLDIPAGQTIA
                     LVGTTGAGKSTLAKLVSRFYDPSEGEVTLDGVDLRMLHPKDLRRAIVMVTQEAYLFSG
                     TVADNIALGKPDATLDEIRAAARAVGADEFITSLPDGYDTDVNKRGGRVSAGQRQLIS
                     FARAFLADPAVLILDEATASLDIPSERLIQDALQTLLADRTAIIIAHRLSTVAIADRV
                     LVMEHGRIIEDDTPDALIAGTGKFAQLHAAWQETLV"
     gene            complement(2024..3970)
                     /locus_tag="VN041_07815"
     CDS             complement(2024..3970)
                     /locus_tag="VN041_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU28984.1"
                     /translation="MSATSSSSSLSTFAALWRLKPFIRPIFGRLIGGAASALAAAVIA
                     LMIPIVLEQIVKGLGDVSGAHLNGGVIGLIIAGAFGVLALALGEAAMVWLRRWFVLTP
                     ATEVEYRMRAELYSRLQNLPVAFHDRWQSGQLLSRMMQDLSLIRRWIAFGLILLVVNI
                     LTIIIGALLLFRWHWMLGAIFIATGIPLWIAGYLFEKRYGTLARRSQDQAGDLATSVE
                     ESVHGIRVLKAFGRGKHALTRFSRQAETLRETELGKARAVGQIWFWLDLMPQLAFGLS
                     LITGIWLISTGAIDVAELFAFFAMATVLRWPIESIGFLFSFMLDARTATDRVFDIYRE
                     TNTITDPEKPVHLAKPRGALAFEGARFRYQDAGPSERDLLDGIDLVLEPGETMALVGL
                     TGSGKTTLTTLPARLYDVTGGRITLDGVDIRDFELAELRRHIAMAFEDATLFSASVRE
                     NVLLGRADLDLHSEEADRVLREALDVAQAGFADSLPDGLETIIGEEGLSLSGGQRQRL
                     ALARAVAAAPKVLVLDDPLSALDVDTEALVEEALRHVLAETTALIVAHRPSTVALADR
                     VALLEKGRITAVGKHSDLLRTSAHYRHVISSLEAEEAARTGTIPIITEAVAEAHRASA
                     TASADRETAVPADEASATEEEVQA"
     gene            4391..>5186
                     /locus_tag="VN041_07820"
     CDS             4391..>5186
                     /locus_tag="VN041_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU28985.1"
                     /translation="MQENADELIADVQRLVSCESPSHDLEAVGRSADAVAEIGIRLLG
                     AAPERVVIDGCTHLRWRFGTAARVLVLGHHDTVWPLGSLRAHPAEVVDGRLRGPGSFD
                     MKAGLVMALHAVAALPDRDGVTVLVTGDEEPGSPTSRVLIEQEARGCEAALILEAAGP
                     GGALKTARKGVSRYEVHVHGRAAHAGLEPENGVNATIELSHQVQAVAALSDPVRGTTV
                     TPTTANGGTTSNTVPARASFSVDVRAASVAEQHRTHDAITALRPRLT"
BASE COUNT          826 a         1792 c         1829 g          739 t
ORIGIN      
        1 gcagctcgag ccgggccgcg tcgtacccga gccgcttgcc gttcaccgtc tgccgcctcc
       61 gcaccgtcgc gaacccgatc gcccctgcgc cggctgcagc cgtcggcgcg atccaccaca
      121 gctcggccgc cgcagcccag atcggatcca ctccttcaac ctaacagccc gtccggccgc
      181 gccgaagggc gggacggacg ggctgtcggg gaggcgggtc agaccagggt ctcctgccag
      241 gccgcatgca gctgggcgaa cttgccggtg cccgcgatca gggcatccgg ggtgtcgtcc
      301 tcgatgatcc tcccgtgctc catcaccagc acgcgatcgg cgatcgcgac ggtcgacagg
      361 cggtgcgcga tgatgatggc cgtcctgtcc gccagcagcg tctgcagcgc gtcctggatc
      421 agccgctccg acgggatgtc cagcgatgcg gtggcctcgt cgaggatcag caccgccggg
      481 tcggcgagga acgcgcgcgc gaacgagatc agctggcgct gtcccgccga gacgcgcccg
      541 ccgcgcttgt tcacgtcggt gtcgtagccg tccggcagcg aggtgatgaa ctcgtccgcg
      601 cccacggccc gggccgccgc acggatctcg tccagcgtgg cgtcgggctt gccgagggcg
      661 atgttgtccg ccacggtccc gctgaacaga tacgcctcct gcgtgaccat gacgatcgcg
      721 cggcgcagat ccttcggatg cagcatccgc aggtcgacgc cgtccagcgt gacctcgccc
      781 tcgctcggat cgtagaaccg cgacacgagc ttggcgagcg tcgacttccc ggcccctgtc
      841 gtgcccacca gcgcgatggt ctgcccggcg gggatgtcga gcgagaagct cggcaggatc
      901 gtcttgtcgc cgttgtaccc gaaggtcacg tcgtcgaagc ggatcgcgcc ccgcgcctcc
      961 cacagatcga tcggcttctg cgggtccgga accgtcggca cctcctcgag cacgcccgac
     1021 accttctcca gcgccgccgt ggcggactgg taggagttca ggaacatcgc gatctcctgc
     1081 atgggcgcga agaagttgcg cacgtacagc acggtcgcca gcaggacgcc gacgccgagg
     1141 gcgccgtcgg cgacgcggag gccgccccac agcaccacca gggtgatcgt cagcgccgag
     1201 accgccatca gtccgggctc gaacgtgccg aacagcacca tggaacgccg gttgatgtcg
     1261 cggtagtcgc ccgccacctt gcggaaggcg acgtcgttgc gcggctcctt gcggaacgac
     1321 ttgaccgcgc ggatgcccgt catcgtctcc acgaactgca cgatcacctt ggcgctgatc
     1381 acccgcgact cgcggtacac gagctgcgag cggcggtaga accagcgcat cagcagcacc
     1441 agcggaacgc ccgccagcag cagcacgacg cccgcctgcc agtcggcgat caccagcgcg
     1501 atgaacgtga agatgccgaa cagcacgccc gagacgaggt tgttcaggcc accgtccagc
     1561 agctcgcgga tcgagtccag gtcgctggtc tgccgggaga tgatccggcc cgacgtgtac
     1621 gactcgtgga actccagact cagccgctgc gtgtgcagga agatgcgctt gcgcaggtcg
     1681 agcagcacgg cctgcgtgat gcgggccgcg agcacgacgt agaagccgat cagcgcggaa
     1741 cccacgatgc cggcgaacag gtacaccgcg gtgaccatga tcgcaggcat ccagtccgcg
     1801 ttcttcagca gctgcggcag cgcggagtcg agcgcgatgc cgatcaggat cggccccgcg
     1861 acctgcagcg cggtcgagac gaccagcacg atcgcggcga tcacgatgcg caggcgcagc
     1921 gggtggatca gcgagccgag caggcgcagc gagcgctgcc ggatggcacg gctctcctcc
     1981 ttcgtcaggt ccgagcggtc ctcgtcccgg gttcccgtga tggtcatgcc tgcacctcct
     2041 cttcggtggc ggatgcttcg tccgcgggca ccgcggtctc gcggtcggcg gatgccgtag
     2101 cggacgccct gtgcgcctct gcgacggcct cggtgatgat cgggatggtc ccggtgcgag
     2161 cggcctcctc ggcctccagg ctcgagatca cgtgacggta gtgcgcgctc gtgcgcagca
     2221 ggtcggagtg cttcccgacc gccgtgatcc tgcccttctc gagcagcgcg acccggtccg
     2281 cgagcgcgac ggtcgacgga cggtgcgcga cgatcagcgc cgtggtctcg gccaggacgt
     2341 gccgcagggc ctcttcgacg agcgcctcgg tgtcgacgtc gagcgccgac agcgggtcgt
     2401 cgaggacgag caccttgggt gcggcggcga ccgcgcgggc cagggccaga cgctgccgct
     2461 gaccgccgga gaggctcagc ccctcctcgc cgatgatcgt ctcgagcccg tccgggagcg
     2521 agtccgcgaa cccggcctgc gccacgtcga gcgcctcgcg cagcacgcgg tcggcctcct
     2581 cgctgtgcag gtcgaggtcg gcccggccca gcagcacgtt ctcgcgcacg gatgccgaga
     2641 acagcgtggc atcctcgaag gccatcgcga tgtgccggcg cagttcggcc agctcgaagt
     2701 cgcggatgtc cacgccgtcg agcgtgatgc ggccacccgt cacgtcgtac aggcgcgcgg
     2761 gcagcgtggt gagcgtggtc ttgccggacc cggtcaggcc caccagggcc atggtctcgc
     2821 ccggctcgag caccaggtcg atgccgtcga gcaggtcgcg ctcggacggg ccggcgtcct
     2881 gataccggaa gcgggcgccc tcgaacgcga gtgcgccgcg cggcttcgcc aggtgcaccg
     2941 gcttctcggg gtcggtgatg gtgttggtct cgcggtagat gtcgaacacg cggtccgtcg
     3001 ccgtgcgagc gtcgagcatg aacgagaaca ggaagccgat cgattcgatc ggccagcgca
     3061 gcacggtggc catcgcgaag aacgcgaaca gctcggcgac gtcgatggct ccggtcgaga
     3121 tcagccagat gccggtgatc aggctcaggc cgaacgcgag ctgcggcatg aggtcgagcc
     3181 agaaccagat ctggcccacc gcacgggcct tgcccagctc ggtctcacgc agcgtctcgg
     3241 cctgtcggct gaaccgggtc agcgcgtgct tgccccggcc gaaggccttc agcacccgga
     3301 tgccgtggac gctctcctcc acgctggtgg cgaggtcgcc ggcctggtcc tggctgcgtc
     3361 gcgcgagcgt gccgtagcgc ttctcgaaca ggtagccggc gatccacagc gggatgcccg
     3421 tggcgatgaa gatcgcaccg agcatccagt gccagcggaa cagcagcagc gcgccgatga
     3481 tgatcgtcag gatgttgacg accagcagga tcaggccgaa cgcgatccag cggcggatca
     3541 ggctgagatc ctgcatcatg cggctgagca gctgcccgga ctgccagcgg tcgtggaaag
     3601 cgaccggcag gttctgcagc cgcgagtaca gctcggcccg catccggtac tccacctcgg
     3661 tagcgggggt gagcacgaac cagcggcgca gccacaccat ggcggcctcg ccgagtgcga
     3721 gggcgagcac gccgaaggcg ccggcgatga tcagtccgat cacaccgccg ttcaggtgcg
     3781 cgccggagac gtcgcccagc cccttgacga tctgctcgag cacgatcggg atcatcaggg
     3841 cgatgacggc cgcggcgagc gcactggccg caccgccgat gaggcggccg aagatcggcc
     3901 ggatgaacgg tttcagtcgc cagagtgcgg cgaacgttga cagggacgac gaagaagagg
     3961 tggcagacat gactctcggt tgcgtagcga tcaggtgacg aagacagggg gccggatgct
     4021 cggcaatccg gtgtggcggg cgggcggtgc tgctccgtga cggagggccg ccggccgcgt
     4081 tcagtcgcgc aggcggggtg cgcgcgagcc gagggcgatg aacgtcgtgg cagtcgtcgt
     4141 ttccatggtg tcctcctcag gctcggcttc gtcgaaccga tcggtgcgac agccttcgag
     4201 cctatgcctg tgtccctcct gaaggcaaga ggaaatccct ttccggtgct gccgatgctc
     4261 tcgctgcggc gcgctcatcg agcccgacca aagcaggcgc cggcattcgg tcattccgac
     4321 agacggcacg cgccggccgg accgcaggat ggactcccag ggccgcacag gcgacagcaa
     4381 aggagcatcg atgcaggaga acgccgacga gctgatcgcg gacgtccagc gcctcgtgag
     4441 ctgcgagtcg ccctcgcacg acctcgaggc ggtcggacgc agcgcggacg ccgtcgcgga
     4501 gatcggcatc cggctgctgg gcgcggcacc cgagcgcgtg gtgatcgacg gctgcaccca
     4561 cctgcggtgg cggttcggga ctgcggcacg agtgctggtg ctcgggcatc acgacacggt
     4621 gtggccgctg ggatcgctcc gggcgcaccc ggcagaagtc gtggacggca ggctgcgcgg
     4681 gccgggatcg ttcgacatga aggcagggct cgtcatggcc ctgcacgcgg tcgccgcact
     4741 gcccgatcgc gacggcgtga ccgtgctggt gaccggcgac gaggagcccg gatcgcccac
     4801 ctcgcgtgtg ctgatcgagc aggaggcccg cggctgcgag gccgcgctga tcctggaggc
     4861 cgccggaccc ggcggcgcgc tgaagaccgc acgcaaaggg gtctcgcggt acgaggtgca
     4921 cgttcacggg cgtgcggcgc acgccggtct ggagccggag aacggcgtca acgccaccat
     4981 cgagctctcc caccaggtgc aggccgtggc tgcgctgtcc gatcccgtac ggggcaccac
     5041 ggtgacgccg acaacggcga atggcgggac cacgtcgaac acggtccccg cccgggcctc
     5101 gttcagcgtc gacgtccgcg ccgcctcggt ggcggagcag caccgcacgc acgacgccat
     5161 caccgcgctg cgtccccgcc tcaccg
//
LOCUS       DATMSX010000259         7216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_60829,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000259 DATMSX010000000
VERSION     DATMSX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60829"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VN041_07825"
     CDS             <1..402
                     /locus_tag="VN041_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU28986.1"
                     /translation="SVQPAHAVDDRDLADDLWREQQAIGYPLASLMNAGVPLRFGSDA
                     PVAVLDPWATIAAAVFRTGDERVPWHPEERVPVDAALRASGRHGDAVIALGAVADLAL
                     CGMDPRTADRRQLTAMPVAATVLGGRITHRA"
     gene            complement(455..940)
                     /locus_tag="VN041_07830"
     CDS             complement(455..940)
                     /locus_tag="VN041_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HWU28987.1"
                     /translation="MSKAQTVPTTAVDPTVAAAAAQFLSPVVLGLEALTVNGKQAHWH
                     VRGANFVGVHELLDTIVAHAGDFADTAAERIVALGLPIDSRIGTVAEKAGATAVPAGF
                     AQSDELVRAIISDIDAIIADVKAAVDGLDAVDLTSQDVAIEIQRGLEKDRWFLVSHIA
                     A"
     gene            1082..1627
                     /locus_tag="VN041_07835"
     CDS             1082..1627
                     /locus_tag="VN041_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWU28988.1"
                     /translation="MSIAATASVLALSDRAPQLAEDSFVADGARIVGAVTLGARASVW
                     YNAVLRGDSAPIVIGADSNVQDNVSVHVDSGHGVQIGERVSIGHNAVVHGCTIGDGSL
                     IGMGAVVLSGAVIGRGCLVAGGAVVLGGTEVPDGSLVAGVPAKVRRALTDEERQGLIA
                     NAEIYLGHIDTHAAADSRHPS"
     gene            1717..2655
                     /locus_tag="VN041_07840"
     CDS             1717..2655
                     /locus_tag="VN041_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWU28989.1"
                     /translation="MRRVARGFFRTTAPDAAAGLTFYAVLALIPALVAAFSLIGMVGS
                     RGDGVRAVLDVAKDVLAPAALVGIGRALEKVASLGGSGWVLVLSLLLALWSVARYVTA
                     LGRAINRIYGVPEGRVLWKAKPIHLLVTLAVFALVALAAVIAGLSRPLAESLGRALGA
                     GEVALSVWAVARWPVLVLVIVLVIAVLYYFAPNIAHAHFRLVSIGAAVAVLVFALASF
                     GFEYYVAAVAHYDWIYGSFAGIIIFLVWLWIGNMALLLGALVDAELERLRELRAGRPA
                     ERELQVELRDTTRMERNAVNDLRDEREARRFRRSRR"
     gene            2708..2781
                     /locus_tag="VN041_07845"
     tRNA            2708..2781
                     /locus_tag="VN041_07845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2742..2744,aa:Ile,seq:cat)
     gene            2899..3942
                     /locus_tag="VN041_07850"
     CDS             2899..3942
                     /locus_tag="VN041_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28990.1"
                     /translation="MRPSIEIRRARRFWSTGALVAAMALTGCSSPLDVSAAERAVAAT
                     CEALEPLPGVSTASCLVDDGGFDAGIRRRTAVELENGATAEQVRSLVDAWLSSEPGGA
                     EEYAIQGSRATPLQVTLSGRSEASFSVAPGAPAPSVAFVQEWLGRATRGVPIVASVAD
                     TQRISIAQDGLSPAEQAALLDEFGMRTRSDGLVLEIGSGSSGTNGASSVEAPVPATLY
                     EALGQLETVYRTLSNDDPAHELAFTMDANPGRSPMLRLRVPTDLVPAGAPGQPVTRTP
                     GWPTIRAILEAAAPGGAEYGLTIAGRPGDVVGGISTSGCAPDVPGPRPRYDDELQAEW
                     AIIHDVPAVGACE"
     gene            complement(3982..4245)
                     /locus_tag="VN041_07855"
     CDS             complement(3982..4245)
                     /locus_tag="VN041_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28991.1"
                     /translation="MTDTTQEPADEQQPSTDDLEEPSTEKEPDEEPKAPARNDPEPDH
                     QAVGIGVIGGPQTDTDDGRTPGQGTAADSEGREDDEPDLDEQP"
     gene            complement(4314..4901)
                     /locus_tag="VN041_07860"
     CDS             complement(4314..4901)
                     /locus_tag="VN041_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWU28992.1"
                     /translation="MLRLREPRTALANFFASGVLALGDRLGPILWQLPERQEFDSALI
                     GDFLAQLPRSTAEARELARDHDDRVEGRAWLDVDADRPLRYALEPRSQSFANPELVPL
                     LAAHGVALATADTAGRWPRFEAATADFAYVRLHGSAELYASGYTGSELRAWADTCRRL
                     CTAPDGRDVFVYFDNDARGHAPHDAEALARLVDDT"
     gene            complement(4982..5173)
                     /locus_tag="VN041_07865"
                     /pseudo
     CDS             complement(4982..5173)
                     /locus_tag="VN041_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            5314..5520
                     /locus_tag="VN041_07870"
     CDS             5314..5520
                     /locus_tag="VN041_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU28993.1"
                     /translation="MNGRTDDELSSQAAEGDRLEQDRDHWDEPDDPERTASPALPDGV
                     EADPADVAEQSVDVPDDAEDEREP"
     gene            complement(5536..6789)
                     /locus_tag="VN041_07875"
     CDS             complement(5536..6789)
                     /locus_tag="VN041_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU28994.1"
                     /translation="MTTKTTIRRMQGLTIEEHTLTVPLVWDDPADTRTIDVFAAVVAR
                     EGGEDLPYLLFLQGGPGHEAPRAFQNSSSPAWLDAALKEHRVVLLDQRGTGRSTPVGD
                     RDLERGSDAVAEHLTHLRADSIVRDAEAVREHLGAETWNVLGQSFGGFTTLAYLSTDA
                     ASLDRVYFTGGLSTIDRTPDEVYALCYDKMRDASERYYRRFPEHRDRMRQLVDLAASG
                     GIVLPGGEVVSPSRLRSFGSALGTNDGWQTVWSLLELDPASNAFRYDLAAAMPFDGRN
                     PLYFVFHESSYASGHVTRWSAERVEPADFVEDTSLFTGEHVRQEWTETVPAFQPWRDV
                     ALALAEHEWPKIYDRAALEASDARGAAAVYVNDVYVPMEYSLETARLLPGVAAWVTSE
                     HEHNGLRGGPVLEHLVDLAHGRRVR"
     gene            complement(6789..>7216)
                     /locus_tag="VN041_07880"
     CDS             complement(6789..>7216)
                     /locus_tag="VN041_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HWU28995.1"
                     /translation="AELVRIWARPDVGEIDDDGLTPVLDAVRRRGWPAGWDREFDYCE
                     YRGEHWEVKDVKALFNRVFRRAWTDDRPAALAYSARHGGPLYESMAWKGHWDTLAEGC
                     HLYMGWDSNYMMTALQGVLEESGIAAEVFVKYSYIGNVM"
BASE COUNT         1027 a         2451 c         2640 g         1098 t
ORIGIN      
        1 agcgtgcagc cggcgcacgc cgtggacgat cgcgatctcg cggacgacct gtggcgcgag
       61 cagcaggcga tcggctatcc gctcgcctcg ttgatgaacg cgggcgtgcc cctgcgtttc
      121 ggatccgacg cgcccgtcgc cgtcctcgat ccgtgggcga cgatcgccgc cgcggtgttc
      181 cgcaccggcg acgagcgcgt gccctggcat cccgaggagc gcgtgccggt cgatgcggcg
      241 ctgcgcgcga gcggacgtca tggagacgcg gtgatcgcac tgggagcggt ggctgatctc
      301 gcgctgtgcg gcatggatcc gcggacagcc gaccgccggc agctgaccgc gatgccggtc
      361 gccgccacgg tcctcggcgg ccggatcacg catcgcgcct gaacgcgaga agggtgccgg
      421 agctcgcgct ccggcaccct tctgatgcgc tcggtcaggc cgcgatgtgc gagacgagga
      481 accagcggtc cttctcgagg ccgcgctgga tctcgatcgc gacgtcctgg ctggtgaggt
      541 cgacggcgtc gaggccgtcg acggccgcct tcacgtcggc gatgatcgcg tcgatgtcgg
      601 agatgatcgc gcggacgagc tcgtcggact gggcgaagcc ggcgggaacg gcggtggcgc
      661 cggccttctc ggcgaccgtg ccgatgcgcg agtcgatcgg caggccgagg gcgacgatgc
      721 gctccgcggc cgtgtcggcg aagtcaccgg cgtgggccac gatcgtgtcg agcagctcgt
      781 ggacgcccac gaagttggcg ccgcgcacgt gccagtgcgc ctgcttgccg ttgaccgtca
      841 gagcctcgag gccgaggacc acgggggaca ggaactgagc ggcggcggct gccacggtcg
      901 ggtcgacggc ggtggtggga acggtctgtg ccttgctcat gaggtcctcc ggatgcgttg
      961 tgaaacgggc ttgatgtcgt caacgctact catcagagaa cattccgcaa gcaagcgaag
     1021 gcaacgctta ccggggcatt ctgccgcggg attccgcggt tctgagggta gtctcaccta
     1081 catgagcatc gcagccacgg catccgtcct cgctctctcc gaccgcgcac cgcagctggc
     1141 ggaggactcc ttcgtggcgg acggcgcccg catcgtcggg gccgtcaccc tgggggcgcg
     1201 cgccagcgtc tggtacaacg ccgtcctgcg cggggactcc gctccgatcg tgatcggcgc
     1261 ggacagcaac gtgcaggaca acgtgtccgt gcacgtcgac agcggacacg gcgtgcagat
     1321 cggcgagcgc gtctcgatcg ggcacaacgc cgtcgtccac ggctgcacga tcggtgacgg
     1381 ctcgctcatc gggatgggcg cggtcgtgct cagcggtgcg gtgatcggcc gcggatgcct
     1441 ggtggcaggc ggtgcggtgg tcctcggcgg aaccgaggtg ccggacggct cgctggtcgc
     1501 cggcgtcccc gcgaaggtgc gccgcgcact gaccgacgag gagcgccagg ggctcatcgc
     1561 caacgccgag atctacctcg gccacatcga cacgcatgcg gcggctgatt cccgccaccc
     1621 gtcgtgagcc gcgctcgacg cgccgtggag cggtccgagc gcggggacgg ggagaggtcg
     1681 tcaacgagtc cacggggcgg acaggtcgcg ttcgtcgtgc ggcgcgtcgc ccgggggttc
     1741 ttccggacga cggcaccgga tgccgccgcc gggctgacct tctacgccgt actggccctc
     1801 atcccggcgc tggtggcggc gttctcgctg atcgggatgg tcgggagcag aggagacggc
     1861 gtgcgagccg tgctcgacgt cgccaaggac gtgctcgcgc ccgccgcgct cgtcggcatc
     1921 ggccgagcac tggagaaggt ggcgtcgctc ggtgggtcgg gctgggtgct ggtgctgtcg
     1981 ctgctcctcg ccctctggtc ggtcgcgcgc tacgtgacgg cgctcggccg tgcgatcaac
     2041 cggatctacg gcgtgccgga ggggagggtg ctgtggaagg cgaagccgat ccacctgctg
     2101 gtcacgctcg cggtgttcgc cctggtggcg ctggccgccg tgatcgcggg actcagccgg
     2161 ccgctcgccg agtccctggg acgggcgctc ggagccgggg aagtggcact gagcgtgtgg
     2221 gcggtggccc gctggccggt gctggtcctg gtgatcgtcc tcgtgatcgc cgtgctctac
     2281 tacttcgcgc cgaacatcgc ccacgcgcac ttccgcctgg tcagtatcgg tgcggcggtg
     2341 gcggtcctgg tcttcgcact ggcatcgttc gggttcgagt actacgtcgc cgctgtggcg
     2401 cactacgact ggatctacgg gtcgttcgcc gggatcatca tcttcctcgt gtggctgtgg
     2461 atcgggaaca tggcgctcct gctcggggcg ctggtcgacg cagaactgga gcgcctccgc
     2521 gagctgcgag ccggacgccc cgcggagcgg gagctgcagg tcgaactgcg cgacaccacc
     2581 aggatggagc ggaacgccgt gaacgacctc agggacgagc gcgaggcccg ccgattccgg
     2641 cgctcccggc gctgacatcg cccgacgacg atgcgcgcgg tgcggcaccg gtaggctggt
     2701 gctctacggg gcggtggcca agctggtgaa ggcagtgggc tcataaccca acgatcgcgg
     2761 gttcaagtcc cgcccgccct accgacatcg acttctctcc acggcatgcg cacgccgctc
     2821 cgagaggagc gtccatggcc gcgggcgacc gtttcggggt cccctacgat gggcgacatg
     2881 ggctgggagg ggagggccat gcggccgtcg attgagatcc gcagggcgag acggttctgg
     2941 tcgaccggag cgctcgtggc ggcgatggcc ctcacgggct gctcgtcacc gctcgacgtc
     3001 tccgcggcgg agcgtgcggt ggcggcgacg tgcgaggcgc tggagccgct ccccggcgtg
     3061 agcacggcgt cgtgcctggt cgatgacggc gggttcgatg ccggcatcag gcggaggacg
     3121 gcggtggagc tcgagaacgg ggccacggcc gagcaggtcc gcagcctcgt cgacgcctgg
     3181 ctgtcatcag agcccggcgg ggcggaggag tacgcgatcc agggctcgcg tgcgaccccc
     3241 ttgcaggtga ccctctcagg acgcagcgag gcctcgttct ccgtcgctcc cggcgcacca
     3301 gccccgagcg tcgcgttcgt gcaggaatgg ctgggacgcg cgacccgggg agtgccgatc
     3361 gtcgcatccg tcgccgacac tcagcggatc tccatcgccc aggacgggct ctcgcccgcg
     3421 gagcaggcgg cgcttctcga cgagttcggg atgcgcacga ggagcgacgg tctcgtgctg
     3481 gagatcggca gcggctcctc tgggacgaac ggcgccagct ccgtcgaggc acccgtaccg
     3541 gcgacgctgt atgaggcgct ggggcagctc gagacggtct accggaccct gtcgaacgac
     3601 gatccggccc atgagctcgc cttcaccatg gacgcgaatc cggggcggtc gccgatgctc
     3661 cggcttcggg tcccgactga cctggttccg gccggcgctc cgggccagcc cgtcacccgg
     3721 acacccggct ggccgacgat ccgcgcgatc ctcgaggctg ccgcgccggg cggtgcggag
     3781 tacggtctga cgatcgctgg tcgcccgggc gacgtcgtcg gcggcatcag cacgtccggc
     3841 tgcgctcccg acgtgcctgg gcctcgcccc aggtacgacg atgaactgca ggccgagtgg
     3901 gcgatcatcc acgacgtccc ggcggtgggc gcctgcgagt gaggcgccca ccgctcggag
     3961 acggagaggt ctgatcgctt ctcagggctg ctcgtccagg tcgggctcgt cgtcctcccg
     4021 gccttcgctg tccgcggcgg tgccctgacc cggggtccgg ccgtcgtccg tgtcggtctg
     4081 ggggccgccg atcacgccga tccctacggc ctggtggtcc ggttccgggt cgttccgcgc
     4141 gggggccttc ggctcctcgt cgggctcctt ctccgtgctc ggctcctcca gatcgtcggt
     4201 gctgggctgc tgttcgtcag caggttcctg agtcgtgtct gtcatgaggc cgagtgtcgc
     4261 gccagagacc cgatgcggga agcgccttga caggacggtg tccgaccatc ccgtcaggtg
     4321 tcgtcgacga ggcgggcgag cgcttccgca tcgtgcggcg catggccgcg tgcgtcgttg
     4381 tcgaaataga cgaacacgtc gcggccatca ggggcggtgc acaggcgccg gcaggtgtcg
     4441 gcccacgccc gcagctcaga gccggtgtag ccgctcgcgt acagttctgc cgatccatgc
     4501 agcctcacat aggcgaagtc ggccgtggct gcctcgaagc gcggccagcg gcccgcagtg
     4561 tcggcggtgg cgagcgcgac gccatgggcg gccaggaggg gaacgagctc cggattcgcg
     4621 aagctctgcg aacgcggctc gagggcgtat ctgagcggac ggtccgcgtc cacatccagc
     4681 caggcacggc cctcgacccg gtcgtcatga tcgcgggcca gttcccgcgc ctcggccgtc
     4741 gatcggggca gctgtgcgag gaagtccccg atcagcgcac tgtcgaactc ctgccgctcc
     4801 ggcagctgcc agaggatcgg ccccaggcgg tctccgagcg ccagcactcc ggaggcgaag
     4861 aagttcgcga gtgccgtgcg cggctcgcgc aggcgcagca tgtgcgtgat gtaccgggag
     4921 ccaccgcgaa cacgaatccc tcggggacac tgtcccgcca gcgcaggtag ctggcgggcc
     4981 gctgcaagga gtagaagctg ccgttcagct cgaccgtcga gaaacgctcg ccgatgaagc
     5041 tcagctcctg gcgctgcgcg agcccgcgcg ggtagaagtc gcctcgccac gaggcgtatc
     5101 gccagcccga ggtgcccacg cggatgcgac ccgcgcctcg agccgccccc tcgtccgcgc
     5161 gaatggccgc catccgtcct ccctccgtcg catcgacacg ctatccgggt ggcgcgcatc
     5221 ccgcaccggg gttgacgcca cgcatccgca tccccctacc gtgggagatc accacgaggg
     5281 atcctggagg tcaggatgaa ggaaggagtg cgcatgaacg gccgaacaga cgacgagctc
     5341 tccagccagg cagccgaagg agaccggctg gagcaggaca gggaccactg ggacgagccg
     5401 gacgatcctg agcggactgc gtcgccggcg ctcccggacg gcgtcgaggc cgatccggcg
     5461 gacgtcgccg agcagtccgt ggacgtcccc gacgacgccg aggacgagcg cgagccctga
     5521 gccgcggacc gcgggtcagc gcacgcggcg cccgtgcgcg agatcgacga ggtgctccag
     5581 gacggggccg ccgcggagtc cgttgtgctc gtgctcgctg gtgacccatg cggccacgcc
     5641 ggggagcagc cgtgcggtct cgagcgagta ctccatcggc acgtacacgt cgttgacgta
     5701 gacggcggcg gctccccggg catcggatgc ctcgagcgcg gcgcggtcgt agatcttcgg
     5761 ccactcgtgc tcggcgagcg ccagcgccac gtcgcgccac ggctggaacg cgggaaccgt
     5821 ctcagtccac tcctggcgca cgtgctcacc cgtgaacagc gaggtgtcct cgacgaagtc
     5881 ggccggttcc acgcgctcgg cggaccagcg ggtgacgtgg cctgatgcgt agctcgactc
     5941 gtggaagacg aagtacagcg gattgcggcc gtcgaacggc atggccgcgg cgaggtcgta
     6001 acggaaggca ttcgacgcag ggtccagctc cagcagcgac cagaccgtct gccagccgtc
     6061 gttggtgccc agtgccgagc cgaacgagcg caggcgcgag ggggagacca cctcgccgcc
     6121 gggcagcacg atgccgccgg atgctgcgag gtcgaccaac tggcgcatgc ggtcgcggtg
     6181 ctcggggaag cgccggtagt agcgctccga ggcgtctcgc atcttgtcgt agcagagcgc
     6241 gtacacctcg tccggcgtgc ggtcgatcgt gctgagtccg ccggtgaagt acacacggtc
     6301 gagcgatgcg gcatccgtcg acagataggc gagcgtggtg aacccgccga aggactgccc
     6361 gagcacgttc caggtctcag cgcccaggtg ctcgcggacg gcttccgcgt cgcgcacgat
     6421 cgagtcggcg cgcagatgcg tgaggtgctc ggccacggca tccgaacccc gctccagatc
     6481 gcggtctccg accggcgtcg agcggccggt gccgcgctgg tcgagcagga cgacacggtg
     6541 ctccttgaga gccgcatcca accaggcggg tgacgacgag ttctggaacg cgcgcggtgc
     6601 ctcgtgcccc ggccctccct gcaggaagag caggtacggc aggtcttcgc cgccctcacg
     6661 ggcgacgacc gccgcgaaga cgtcgatggt gcgggtgtcg gccgggtcat cccacaccag
     6721 cgggacggtg agggtgtgct cctcgatcgt caggccctgc atgcggcgga tcgtcgtctt
     6781 cgtcgtcatc acatcacgtt cccgatgtag gagtacttca cgaacacctc cgcggcgatg
     6841 ccggattcct ccagcacccc ctgcagggcg gtcatcatgt agttcgagtc ccatcccatg
     6901 tagagatggc agccctcggc gagggtgtcc cagtggccct tccaggccat cgactcgtac
     6961 agcggcccgc cgtggcgtgc actgtaggcg agggcggcgg gccggtcatc ggtccaggcg
     7021 cgccggaaga cgcggttgaa cagcgccttg acgtccttga cctcccagtg ctcgccgcgg
     7081 tactcgcagt agtcgaactc gcgatcccag ccggccggcc agccgcgccg gcgcacggcg
     7141 tccagcaccg gtgtcaggcc gtcgtcgtcg atctcgccga cgtcgggccg cgcccagatg
     7201 cgcaccagct ccgcgg
//
LOCUS       DATMSX010000260         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_51264,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000260 DATMSX010000000
VERSION     DATMSX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51264"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1427)
                     /locus_tag="VN041_07885"
     CDS             complement(156..1427)
                     /locus_tag="VN041_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU28996.1"
                     /translation="MIKARLLAAVGMTAVAALALAGCSSAADAGDGGNSNEKVTLRVL
                     VNVTPNLTEKFWNDLVAPFEAKNKNIDVVIQNPGAEGVSAAIPRLLASGDAPDIVQSL
                     APNGKLAPELVDLSKYEWAKKGPLAEQYSMDGKYLMAGIGVQLQSLFFYNKTAFKEAG
                     ITEVPTTVDELTEDLGKLKDAGWTPIQEGGDWMSSATLQALALPTIVSENPKWFQDVS
                     SGKVEFGKTYGPAIETFADWVAKGYVPKDALGIKYPDAEQAFLSGKTAIYSMGSWFMG
                     SQAKATDSADIGVFRAPATKAGDKPAMGANIASPYSILKASKNQEASAKLIEFLTTDK
                     KAVADQLEVDGNFRDGYEYDMTPLGEELLKIVADTPASDYTPTGGGYGERTMPDGFSG
                     ELNTQTQALMGGTPAADVIKSMDAWFAANAG"
     gene            complement(1568..2104)
                     /locus_tag="VN041_07890"
     CDS             complement(1568..2104)
                     /locus_tag="VN041_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWU28997.1"
                     /translation="MIFTSDTAEALRAAVALVNSAEQPDTLETIDDYEGFLAEYPYTG
                     RLDRDAAELRSLRDLRPRLRGMLLAPRDEMVAHVNAALADVTLTPRLTRHDAADWHLH
                     AVADDHPLAERILVETAMALIDVVRADEGSRLDVCADADCQAIALDLSRNRSKRYCST
                     TCANRNAVAAYRARRASL"
     gene            2144..3100
                     /locus_tag="VN041_07895"
     CDS             2144..3100
                     /locus_tag="VN041_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU28998.1"
                     /translation="MGHVAHDTRVGAGLLVALGAAFSFGMSGAWARGLIDAGWTPGAA
                     VTARVWVAALVLLVPTILALRGRWSVLRRNAGMVIGYGLLAVTATQLFYFQAVAVMDV
                     GIALLVEYTAPIAVVLWLWLRRGEKPTRRSILGAVIAFAGLVLMLDILTGARVDAAGI
                     LWSLGAMVGAATYFVLSAREDTGMPPIVLAGSGLLLGAIGLTVAGVTGILPMAFTFGD
                     VAYRFGTVPWFVPVLAIGLLATALAYVLGIASTRMLGSRPASFVALAEVVAALLFGWM
                     LLGQLPEPLQLLGAALVLAGVVVVKLGEPAVAEEGEPLAVPV"
BASE COUNT          397 a         1015 c         1169 g          593 t
ORIGIN      
        1 cggattctcg ggtgaactga acacccagac ccaggcgctg atgggcggaa ccccggccgc
       61 cgacgtgatc aagtccatgg acgcctggtt cgccgcgaac gcaggctgat cgccgtggcg
      121 ggcgggacga cgcgtcgtcc cacccgccac ggcgatcagc ctgcgttcgc ggcgaaccag
      181 gcgtccatgg acttgatgac gtcggcggcc ggggttccgc ccatcagcgc ctgagtctgg
      241 gtgttcagct cacccgagaa cccgtcgggc atggtgcgct cgccgtagcc gccgccggtg
      301 ggcgtgtagt cggatgccgg ggtgtcggcg acgatcttga gcagctcctc gccgaggggc
      361 gtcatgtcgt actcgtagcc gtcgcggaag ttgccgtcga cctcgagctg gtcggcgacc
      421 gccttcttgt ccgtggtcag gaactcgatc agcttggccg aggcctcctg gttcttgctc
      481 gccttgagga tcgagtacgg gctggcgatg ttggcgccca tggcgggctt gtcgccggcc
      541 ttggtcgcgg gagcgcggaa cacgccgatg tcggcggagt cggtggcctt cgcctgcgat
      601 cccatgaacc agctgcccat ggagtagatc gcggtcttgc cggagaggaa cgcctgctcg
      661 gcatccgggt acttgatgcc cagggcgtcc ttggggacgt aacccttcgc gacccagtcg
      721 gcgaaggtct cgatggcggg cccgtaggtc ttgccgaact ccaccttgcc ggaggagacg
      781 tcctggaacc acttcgggtt ctcggagacg atggtcggca gggcgagggc ctgcagggtg
      841 gcgctggaca tccagtcgcc gccctcctgg atcggggtcc agccggcgtc cttcagcttg
      901 ccgaggtcct cggtgagctc gtcgaccgtg gtgggcacct cggtgatgcc cgcctccttg
      961 aaggcggtct tgttgtagaa gaacaggctc tgcagctgca cgccgatgcc ggccatcagg
     1021 tacttgccgt ccatggagta ctgctcggcg agcgggccct tcttggccca ctcgtacttg
     1081 gacaggtcga cgagttcggg tgcgagcttg ccgttcgggg cgagggactg cacgatgtcg
     1141 ggcgcgtcgc cggaggcgag gaggcgcggg atcgcggccg agacgccctc ggcgccgggg
     1201 ttctggatga cgacgtcgat gttcttgttc ttcgcctcga acggggcgac gaggtcgttc
     1261 cagaacttct cggtgaggtt cggcgtgacg ttgacgagca cacgcagtgt gaccttctcg
     1321 ttcgagttgc cgccgtcgcc ggcgtctgct gcgctgctgc agccggccag cgccagggcg
     1381 gcgaccgcgg tcatgccgac tgcggcgagg agtcgagcct tgatcatcgt tgatcctttc
     1441 gggatgcagg ggagggtgct tccagggaca ttacactaat cgataatcgt ttgtgaagag
     1501 cgtgcgaagg attgccagag gcgctctccg ggtaggcggt gccgcgcggg ggtggggtcg
     1561 cgcggtgtca gagcgaggcg cggcgggcgc gataggcggc cacggcgttg cggttcgcgc
     1621 aggtggtgga gcagtaccgc ttggagcggt tgcggctgag gtccagcgcg atcgcctggc
     1681 agtcggcatc cgcgcagacg tccagccggc tgccctcgtc ggcgcggacg acgtcgatca
     1741 gtgccatggc ggtctcgacg aggatccgct cggccagcgg atggtcgtcg gcgacggcgt
     1801 gcaggtgcca gtcggcggcg tcgtgacgcg tcaggcgcgg cgtgagcgtc acgtctgcga
     1861 gggcggcgtt gacgtgcgcg accatctcgt cgcgaggggc gaggagcatg ccgcgaaggc
     1921 gcggtcgaag atcgcgcagg ctccgcagct cagcggcgtc gcggtcgagt ctgccggtgt
     1981 acgggtactc ggcgaggaag ccctcgtagt cgtcgatcgt ctcgagggtg tccggctgct
     2041 cggcggagtt caccagtgcg acggcggcgc ggagcgcctc cgcagtgtca ctggtgaaga
     2101 tcatgttgac accttacatc tcccaggaat agtgtcatct cccatggggc atgttgcaca
     2161 tgacactcgc gtgggcgcgg gtcttctcgt cgcgctgggt gcggcgttct cgttcggcat
     2221 gtccggagcg tgggcgcgtg gcctgatcga tgcgggatgg acgccgggcg cggccgtgac
     2281 cgcgcgggtg tgggtggcgg ccctggtgct gctggtgccg acgattctcg cgctgcgcgg
     2341 gcgctggtcg gtgctgcgcc gcaacgccgg catggtcatc ggctacgggc tcctcgccgt
     2401 cacggccacg cagctgttct acttccaggc ggtcgccgtg atggatgtcg gcatcgccct
     2461 gctggtcgag tacaccgctc ccatcgcggt ggtgctctgg ctctggcttc gccgcggcga
     2521 gaagcccacg cgtcgcagca tcctgggcgc ggtgatcgcg ttcgcgggcc tggtgctcat
     2581 gctcgacatc ctcaccggtg cacgggtcga cgccgccggc atcctgtggt cgctcggcgc
     2641 catggtgggc gccgcgacct acttcgtgct ctccgcccgc gaggacaccg gcatgccgcc
     2701 gatcgtgctt gccgggtccg gtctgctgct cggtgcgatc gggctgaccg tcgccggggt
     2761 caccggcatc cttccgatgg cgttcacctt cggcgatgtc gcctatcgct tcggcaccgt
     2821 cccgtggttc gttcccgtcc tggcgatcgg cctgctggcg accgccctcg cctacgtgct
     2881 cggcatcgcc tcgacgcgga tgctcggctc gagaccggcc tccttcgtcg ccctcgccga
     2941 ggtcgtcgcg gcgctgctgt tcggctggat gctgctgggg cagctgcccg agcccctcca
     3001 gctgctcggc gccgccctgg tgctcgcggg cgtcgtggtc gtcaagctcg gcgagccggc
     3061 ggtcgccgag gagggggagc ccctcgcggt gccggtgtga ctcgccgacg ttgccattca
     3121 cggtgaccgc aggatgctgg ccttcgagga tccccggacc aacagcatcc gcac
//
LOCUS       DATMSX010000261         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_155614,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000261 DATMSX010000000
VERSION     DATMSX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155614"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VN041_07900"
     CDS             complement(<1..699)
                     /locus_tag="VN041_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HWU28999.1"
                     /translation="MTPSRSSAVPRDEELRLPRPPGVIRRFWARHPLFADILIAVLCL
                     LVSVGTSTIPAPGTPPAPPAEVVFAVLMLVGSSVLLVWRRRWPIAVLVAAIATDVTLL
                     FVMRSAGAPLFALALYALAVYRSVRAAWIGLGAGTLVIAGLAFAWFGFGGIDLQAAVN
                     AVLSVAVPGLIAALIGINVGNRKRYVEAIIDRSRQLLVERDQQAQLAAAAERDRIARE
                     MHDIVSHSLTVVVAL"
     gene            complement(723..1532)
                     /locus_tag="VN041_07905"
     CDS             complement(723..1532)
                     /locus_tag="VN041_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29000.1"
                     /translation="MTAIQARATHIPAPAGRLSFFRQLRSEYLKLTTVRGTWWSIGIV
                     AVITIAIAFAVTSAISGPVENIMMAVLMPVQFTMLIAAILGVISVTGEYSTGMIRSTF
                     TADPVRGAVVAAKATVLGVFMFVVSLITFSVAAIGISPIAASKDMAVDWADPSQTILP
                     IISASAVMALFALLGVGLGFLLRSGAGAIAVTVGIVFILPTVLGLLGMWLSDLTWISD
                     LAKYLPASLAPTAILPSEGAEMNAAQSLGVLAAWPAAALLGAWAVVRGRDV"
     gene            complement(1529..2473)
                     /locus_tag="VN041_07910"
     CDS             complement(1529..2473)
                     /locus_tag="VN041_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29001.1"
                     /translation="MITAEGLGKRYGDKVAVDDVSFTVRPGSVTGFLGPNGAGKSTTM
                     RMIVGLDRPTTGRVTINGQDYRKLRAPLTEVGVLLDAKAVHTGRSARNHLRAMAATHG
                     ISNSRVDEVIDITGIGSVARKRAGGFSLGMGQRLGIAAALLGDPHTLILDEPVNGLDP
                     EGVRWVREFVRHAASEGRTVLLSSHLMSEMSQTADHVIVLGRGKVLADAALPDLVRAW
                     TTARVHVRSPRAAELADAVAGRDVEIVTADPFTLDVTGLPASRIGDIAAERGIPVHEL
                     TPTSGSLEDAYLALTGDAVEYRTRAVPHTDAPLEGALA"
     gene            2622..2695
                     /locus_tag="VN041_07915"
     tRNA            2622..2695
                     /locus_tag="VN041_07915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2654..2656,aa:Gly,seq:ccc)
     gene            complement(2732..3373)
                     /locus_tag="VN041_07920"
     CDS             complement(2732..3373)
                     /locus_tag="VN041_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013348860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWU29002.1"
                     /translation="MIQVQPLFAFALVAALLTIVPGIDTSLVLRGALTRGHRYGFATA
                     IGICCGALVWGVAAAIGASALLAASELAYRVLTLAGAGYMIWLGGSLIWKSFRRGADA
                     AAHDLPAMRTADSLGRAWLTGFGTNLLNTKVGVFYLATIPQFIPAGTSPALMGMALAA
                     VHALLSIIWFAAIILATAVVSRWLRSPRAVRIIDRITGTVLIGFGVKLAAFPH"
     gene            3427..3885
                     /locus_tag="VN041_07925"
     CDS             3427..3885
                     /locus_tag="VN041_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWU29003.1"
                     /translation="MSYVVDFTNVSTVGLESSPVVEALAGLRANEARYFHNKYEIDFT
                     TEPAEGTDTLAWVTKILADERGIVISSPALEVAEPPVPGIRWAYVFYESGLAINVLYE
                     LTEGGKRAVGFKLSDGMEVPDELGAFKFARQKSKLAGTIRGSYFVIKGEY"
     gene            complement(3899..4441)
                     /locus_tag="VN041_07930"
     CDS             complement(3899..4441)
                     /locus_tag="VN041_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU29004.1"
                     /translation="MNAKYHFVSTFRITGDPELVWRVLSDVERWILWWRCLEVSEVLS
                     EGERTADGDSPAVGSAYRHTVRTPFGYRLRYEIRVTGVESRRSVDAEVSGDLVGRGRA
                     VAEAAGDEATVRFLWLVAVSKPWMRRFSPIARPAFTWAHRRLMADFGEGFTTASGAHL
                     VSTEHVALRPGDASFWVMPE"
BASE COUNT          770 a         1582 c         1643 g          648 t
ORIGIN      
        1 gagcgcgacg acgacggtca gggagtgcga cacgatgtcg tgcatctcgc gggcgatccg
       61 gtcgcgctcg gcggcggcgg ccagctgggc ctgctgatcg cgctcgacga gcagctgccg
      121 cgaacggtcg atgatcgcct cgacgtagcg cttgcgattg ccgacgttga tgccgatgag
      181 cgcggcgatc agcccgggca ccgcgacgga cagcaccgcg ttgaccgcgg cctgcaggtc
      241 gatgccgccg aacccgaacc aggcgaacgc caggccggcg atgaccaggg tgccggcccc
      301 gagaccgatc caggcggccc gcacggagcg gtacaccgcg agcgcataca gcgcgagggc
      361 gaacagcggt gcgcccgccg atcgcatgac gaacagcagc gtcacgtcgg tggcgatggc
      421 cgcgaccagg accgcgatcg gccagcggcg ccgccacacc agcagcacgc tggatccgac
      481 cagcatcagc accgcgaaca cgacctcggc gggcggcgcc gggggtgtgc cgggcgcagg
      541 gatcgtgctg gtgccgaccg agacgagcag gcacagcacg gcgatgagga tgtcggcgaa
      601 cagcggatgc cgtgcccaga agcgacggat cacgccgggc ggacgcggca gccgcagctc
      661 ctcgtcccgg gggacggcgg agctgcggct gggggtcacg ctgtcgactc tacgtgcgcg
      721 cgtcacacat cccgtccgcg cacgacggcc cacgcgccca gcagggcagc ggcgggccag
      781 gcggcaagaa cgccgagcga ctgagccgcg ttcatctcgg ctccctctga ggggaggatc
      841 gcggtcggtg cgagcgatgc cggcaggtac ttcgcgagat cgctgatcca ggtcaggtcg
      901 ctgagccaca tccccagcag tccgaggacg gtcggaagga tgaacacgat tccgaccgtg
      961 acggcgatgg ccccggcgcc cgagcgcagc aggaagccga gccccacgcc cagcagcgcg
     1021 aacagcgcca tgaccgcgga ggcgctgatg atcggcagaa tcgtctgcga cgggtccgcc
     1081 cagtccactg ccatgtcctt cgaagcggcg atcgggctga tgccgatcgc tgcgacgctg
     1141 aaggtgatca gcgagacgac gaacatgaag actccgagca cggtcgcctt ggcggcgacc
     1201 acggcgccac gcacgggatc ggcggtgaag gtggagcgga tcatgcccgt ggagtactca
     1261 ccggtgacgg agatgacacc gaggatggcg gcgatcagca tggtgaactg caccggcatg
     1321 agcacggcca tcatgatgtt ctcgaccggc ccggagattg cggaggtcac ggcgaacgcg
     1381 atcgcgatcg tgatgaccgc gacgatgccg atcgaccacc aggttccacg cacggtggtg
     1441 agcttcaagt actcgctgcg cagctggcgg aagaagctca gccggcctgc gggcgcgggg
     1501 atgtgggtgg cgcgagcctg gatcgcggtc atgcgagtgc tccttcgagc ggggcgtcgg
     1561 tatgcgggac ggcccgggtg cggtattcga cggcgtcgcc ggtgagggcg aggtaggcgt
     1621 cctccagcga gccgctggtg ggggtgagct cgtgcaccgg gattccgcgc tcggcggcga
     1681 tgtcgccgat gcgggatgcc ggcaggccgg tcacgtcgag ggtgaacggg tcggcggtga
     1741 cgatctcgac gtcgcgtccg gcgacggcat ccgccagctc cgcggctctc gggctgcgga
     1801 cgtgcacacg agccgtggtc caggcgcgca cgaggtcggg cagggcggcg tcggcgagca
     1861 ccttgccgcg gccgagcacg atcacgtggt cggccgtctg ggacatctcg ctcatcagat
     1921 ggctggagag cagcacggtg cggccctcgg atgccgcgtg gcgcacgaac tcgcgaaccc
     1981 agcgcacgcc ctcggggtcg aggccgttga ccggctcgtc gaggatgagc gtgtgcgggt
     2041 cgccgagcag cgccgcagcg atcccgagac gctgacccat acccagcgag aagccgccgg
     2101 cacgcttgcg ggcgaccgag ccgatgccgg tgatgtcgat cacctcgtcg acgcgggagt
     2161 tcgagatgcc gtgggtggcc gccatcgcgc gcaggtggtt gcgggcgctt cgcccggtgt
     2221 ggaccgcctt cgcgtccagc agcacgccca cctcggtgag tggtgccctg agcttgcggt
     2281 agtcctgtcc gttgatggtc acccggcctg tcgtgggcct gtccaggccc acgatcatgc
     2341 gcatggtggt ggacttaccc gcgccgttcg gccccaggaa tcccgtgacg ctccccggcc
     2401 gcaccgtgaa ggacacgtcg tccacggcga ccttgtctcc gtacctcttc ccgaggccct
     2461 ctgctgtgat catgccttcg acgctatttc cgcaggtgcc gctgccgcgt ccgcccggag
     2521 gatgaaacct gtgcggggcc tatggcactt tggtgccata ggccccgcgt cgggatcggg
     2581 cccgcgggtt tgttatgctg gccaggcgcc gcaaggcgtg cggggctgta gttcaatggc
     2641 agaacttctg cttcccaagc agacagcgcg ggttcgattc ccgtcagccc ctccacctga
     2701 aggcctccac tgatgtgggg gccttcttcc gtcagtgcgg gaacgccgcc agcttcacgc
     2761 cgaagccgat cagcaccgtg ccggtgatcc ggtcgatgat ccgcaccgcg cgcgggctgc
     2821 gcagccagcg cgagacgacc gccgtcgcga ggatgatcgc ggcgaaccag atgatcgaca
     2881 gcagcgcgtg cacggcggcg agggccatgc ccatcagagc gggcgaggtg ccggcgggga
     2941 tgaactgcgg gatcgtcgcc aggtagaaga cgcccacctt cgtgttgagc aggttggtgc
     3001 cgaagccggt gagccacgca cggccgagcg agtccgccgt gcgcatcgcg ggcaggtcgt
     3061 gcgcggcagc atccgctccc cgccggaacg acttccagat cagcgagccg cccagccaga
     3121 tcatgtaccc ggcgccggcg agcgtgagca cccggtaggc gagctcggat gctgcgagca
     3181 gggcggatgc cccgatcgcc gccgccacgc cccagaccag cgcgccgcag cagatgccga
     3241 tcgcggtcgc gaacccgtac cggtgcccgc gggtcagggc gccgcgcagc acgagcgagg
     3301 tgtcgatgcc ggggacgatc gtcagcaggg ccgccacgag cgcgaaggcg aagagaggct
     3361 gcacctggat cacggatcga tcctagttct gcgacgacca cgcccccgcc ggtatagcgt
     3421 tgccccatgt cgtacgtcgt cgatttcacg aacgtgtcca ccgtcgggct ggagtcatcg
     3481 cctgtcgtcg aggcgcttgc cggtcttcgc gccaacgagg cgcgctactt ccacaacaag
     3541 tacgagatcg acttcaccac cgagcccgcc gagggcaccg acaccctcgc gtgggtcacg
     3601 aagatcctcg ccgacgagcg cggcatcgtc atctcctccc ccgcgctcga ggtcgccgag
     3661 ccgccggtcc cgggcatccg ctgggcgtac gtcttctacg aatcgggcct ggcgatcaac
     3721 gtgctctacg agctgaccga aggcggcaag cgcgccgtgg gcttcaagct ctccgacggc
     3781 atggaggtgc ccgacgaact cggcgcgttc aagttcgcgc ggcagaagtc caagctcgcc
     3841 ggaaccatcc gcggctcgta cttcgtgatc aagggcgagt actgagcggc cgcagccgct
     3901 actccggcat cacccagaaa gacgcatccc cagggcgcag cgcgacgtgc tcggtcgaga
     3961 cgagatgcgc tccggatgcc gtggtgaacc cctccccgaa gtcggccatc agccgacggt
     4021 gcgcccaggt gaacgccggt cgggcgatgg gcgagaaccg tctcatccat ggcttcgaca
     4081 cggcgacgag ccagaggaag cgcacggtcg cctcatcccc cgccgcttcg gcgaccgcgc
     4141 ggccgcggcc gaccagatcg ccggagacct cggcatccac ggatcgccgg gactcgacac
     4201 ccgtgacccg gatctcgtat cgcaggcggt agccgaatgg cgtgcgcacc gtgtgccggt
     4261 aggccgatcc cacagccggt gagtcgccgt ccgccgtccg ctccccctcg gacagcactt
     4321 cggagacctc gagacacctc caccagagga tccagcgctc gacgtcgctg agcactcgcc
     4381 acacgagttc cggatcaccg gtgatccgga acgtgctgac gaagtggtac ttcgcgttca
     4441 tggtctcccc gtctgcgtga tggtccgaac ctactccggg tgtcacaggg ctcagaggaa
     4501 cgcggccccg ccagcggagt cgaatccgcg cagccgcgcg tagtcatcca gggagcgcag
     4561 agccgcccag gccaccacgg cgctggaacg cggccggtcg gcagacggcg tgttcgtgac
     4621 cacggcgcgc acctcgccga gcg
//
LOCUS       DATMSX010000262         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_164768,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000262 DATMSX010000000
VERSION     DATMSX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164768"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..956
                     /locus_tag="VN041_07935"
     CDS             219..956
                     /locus_tag="VN041_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29005.1"
                     /translation="MSMDMADAGRLMAELRETAAELQRELRSVATAAEQMTSVTVEDD
                     RKAVKLTLAKNGAVEALVLDEDWRDTIGEAGLGAVVSLCYQTALGKRFDQLMGGFEEQ
                     DEDDTPVTIPAPQFQLGDPTTSSAAEARSEILDAIDEARDQREAYLNKVNQKGSEVRD
                     AVNSAKTVTVSRRGTTITGITLDERWVRNTNDGIIEREIVRAMNNVMVMAASDRQNTY
                     EGFPAVQRLMEMTRDPQEMLRRMGSIR"
     gene            973..>1654
                     /locus_tag="VN041_07940"
     CDS             973..>1654
                     /locus_tag="VN041_07940"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HWU29006.1"
                     /translation="MGFGDFVGDVVDAVSPVDEIVDAGKNIMEGDLIGALGNVVDAAT
                     PIDEIVSAGSHLFGGPDLGISDITNDLPDKLPVIEGESALPEPSPQPAASSFGEEGKV
                     DVKTEYLRKDAGLWDEAGTTYGTIAGAADGLTIDASAFTFFGVDAAQAYADFQVTMAQ
                     LLHSAPDAHKGFADSLRLTADDYDKTVQKSADDQKKNDPGSANTNNNTGGGGGGGGGG
                     GGGGGGGGG"
BASE COUNT          308 a          500 c          595 g          251 t
ORIGIN      
        1 tggccgaccc gctggacccc gcccgcgcga tcgtgggctg ggggttcggg gccggggcga
       61 cgatctcggt gatcctgctg ctgctcgccc gagcccgcgc ccgtcgcgcc ggcgactgag
      121 tcggatgggc agcgctgcat ggggagtcct ccccctatgg cgctcggacg cctcttcgta
      181 ggattgcttc aacggtccac gacagtaggg agtcccacgt gagcatggac atggcggatg
      241 ccggtcgcct gatggcggag ctgcgtgaga ccgcagcgga gctgcagcgc gagctccggt
      301 cggttgcgac cgcagcggag cagatgacct cggtgacggt cgaggatgat cggaaggctg
      361 tgaagctgac cctcgccaag aacggtgcgg tcgaggctct cgtcctcgac gaggactggc
      421 gtgacacgat cggcgaggcg ggactcggcg cagtggtgag cctgtgttac cagaccgcgc
      481 tcggcaagcg attcgaccag ctgatggggg gcttcgagga gcaggacgag gacgacaccc
      541 ctgtcacgat ccccgcgccg cagttccagc ttggtgaccc cacgacgagc tcggcggctg
      601 aggcgcgcag cgagatcctc gatgcgatcg acgaggcgag ggaccagcgg gaggcttacc
      661 tcaacaaggt caaccagaag ggcagcgagg tccgggatgc ggtgaactcc gcgaagacgg
      721 tcacggtctc gcgccgcgga accaccatca ccgggatcac cctcgatgag cgctgggtgc
      781 gcaacacgaa cgacggcatc atcgagcggg agatcgttcg cgcgatgaac aacgtcatgg
      841 tcatggcggc gagtgaccgc cagaacacgt acgaaggctt cccggccgtt cagaggctga
      901 tggagatgac ccgggatcct caggaaatgc tgcgccgcat gggcagcatc cgctgagaca
      961 aggagcagag caatgggatt tggagatttt gtaggggacg tcgtcgatgc ggtctctccg
     1021 gtcgacgaga tcgtcgatgc cggtaagaac atcatggagg gcgacctcat cggcgcgctg
     1081 ggcaacgtcg tcgacgcggc caccccgatc gacgagatcg tgagcgccgg gtcccacctc
     1141 ttcggaggcc cggacctggg catctcggac atcaccaacg accttccgga caagctgccg
     1201 gtgatcgagg gggagtccgc tcttcccgag ccgtcgccgc agccggccgc atcgtcgttc
     1261 ggcgaggagg gaaaggtcga tgtcaagacc gagtatctgc gcaaggacgc cggtctgtgg
     1321 gatgaagcgg gaaccacgta cggcaccatc gccggtgcgg ctgacggttt gacgatcgac
     1381 gcgtcggcgt tcacgttctt cggcgtcgat gcggcacagg catacgcgga cttccaggtg
     1441 acgatggcgc agctgctgca cagtgctcct gacgcgcaca agggtttcgc agactccctc
     1501 cgtctgacgg ccgacgacta tgacaagacc gtccagaagt ccgcggatga ccagaagaag
     1561 aacgatcccg ggagcgccaa caccaacaac aacaccggtg gcggtggcgg tggcggcggc
     1621 ggtggcggtg gtggcggtgg cggcggcggc ggtg
//
LOCUS       DATMSX010000263         9897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_98619,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000263 DATMSX010000000
VERSION     DATMSX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98619"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VN041_07945"
     CDS             complement(<1..823)
                     /locus_tag="VN041_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWU29007.1"
                     /translation="MLRPPFGVVLAGVVVAALSLLPLIFVITTAVQIGWDQSIALIFR
                     PRVGELLGNTVLLVALAVPLSAVLGVGGAWLVERTSLAGAGFWGAVLAMPLAIPAFVN
                     SYAWVSAIPSLAGLGSGVLLSTLSYFPLVYLPVRAALAGLDPALEQSAASLGRGPVRV
                     FFGTVLPQLRLALLGGSLLVGLHLLSEYGAFALIRFDTFTTAIMDQYRSTFNGSAGSM
                     LAGVLTLLCLLMLVLESRARGRARYARIGSGTAAPPSRVLLRGWQLPAQLGLGALA"
     gene            complement(876..1925)
                     /locus_tag="VN041_07950"
     CDS             complement(876..1925)
                     /locus_tag="VN041_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HWU29008.1"
                     /translation="MNRRLRLLSATAAAAVAALTLAACSGPAATDSGSSTTKPSDETI
                     TVYNAQHETLTKEWIDAFEKETGIEVQVRNGDDTEMSNQIIQEGDRSPADVFLTENSP
                     SMTAVEKAGLFTSPGEEALKNVPEQYRPSSEKWVGIAARSTVFAYNTTKLTEAQLPKS
                     MMDLADPAWKGRWAASPSGADFQAIVSALVQLKGEKAAESWLQAMKTNSVAYKGNSTA
                     MKAVNAGEIDGAIIYHYYWFGDQAQTGENSKNVALHYFKNQDPGAFVSISGGGVLKST
                     KHKAAAQKFLAFITGKKGQEVLQNGTSFEYPVGSDVPANSELVPLTDLDAPKIDPATL
                     NSKKVTDLMTQAGLL"
     gene            2062..3327
                     /locus_tag="VN041_07955"
     CDS             2062..3327
                     /locus_tag="VN041_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWU29009.1"
                     /translation="MRQEWFDEARFGMFVHFGIYSVAARHEWVQSYERLTDDDYRQYF
                     EHFDPDRFDARAIARQAREAGMGYVVLTTKHHDGFCLFDSALTDFSSQQTSGRDLVRE
                     FVDAVRAEGLKVGLYHSVIDWHHPDFTVDWNHPRRDDENAAALNEGRDMGRYREYLHG
                     QVQELLTQYGRIDYLFFDFTYPESRDGWAGKGPDAWDAEALLGMCRELQPDMLVNDRL
                     GIPADFVTPEQYQPTAPLVDEHGAPRVWEACQTLNGSWGYHRDNTDQKSPTLLVQMLA
                     DSVSMDGNMLLNIGPDGRGAIAPRDARTLEEIGRWMLLHRDAIVGAGHAEFTPPREGV
                     YTLRGDRLYLHLFSWPLGFVHLPDLAGRVSFARLLNDGSWLKTSVPDPDQHDTHMTPT
                     LQDEGTLTVHLPVQRPDVLMPVIELTLAD"
     gene            complement(3344..4474)
                     /locus_tag="VN041_07960"
     CDS             complement(3344..4474)
                     /locus_tag="VN041_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU29010.1"
                     /translation="MPSPDDAPTRRIVELIATGAARSRTEIATALGVAPSTVGIRVQS
                     LLDAGILREAGEGTSRGGRRPRVLQLAEAGTILTADLGGGHARIGRHTLGGALDRIET
                     IAIDLSEGPKRTLERVSEVFDRFAENASVRAIGVGLPGPVVVETGSVEQPSRMPGWPG
                     FRVGEHLTARHEVPVIVDNDANLAALGEHTSQLGGDGHSITVKAGTAIGSGIIVDGRV
                     HRGATGAAGDITHTRIDGSGDIPCSCGNTGCLETVASGASLVRQMRERGHQAVHTTED
                     VLALARDADPEATTLVRTAGTHLGQALSGVVNFFNPHAVFLTGSMSGSEPFLAAVRSR
                     VYEACHPLVTQTLRIEAARTGADAILLGAARLAMDHLEMPVA"
     gene            4629..5888
                     /locus_tag="VN041_07965"
     CDS             4629..5888
                     /locus_tag="VN041_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU29011.1"
                     /translation="MKIKRVGAVIVVAGVAVAMAGCSNGPSTGGSGDGDAIVVDMWAG
                     SADDTAALKAQIDVVKKQNPDLKVELRTAPWGDFFTKLTTNMASGNMACVTGMNSGML
                     ASYTDGFVKLTDADLKAAGLAESDFAAGATDILKNKGDLYGLPFDVSTMVTYYNEDQL
                     NAAGAELPKVGWTFDDFEATAKKATTGGKFGFGIGMGDFQWQALPIAKSGTQPVSADG
                     KLQLTDPGFAAAAGWYADLVTKQKVAAPVASASDGGWGENQFTGENAAMAVDGTWNAV
                     GYLKNDAGFKAGLAPLPSGDKGDLSLVLGSGFGIAKTCKNKDAALKMLGSLLSKDSQD
                     YIASSGRSYPARAESQPLYFESLDPAYRDQVKAVFEAAFKNVEGQHVSDNWAKVGTFV
                     QPELVSVYNGQESMKSVLESAQQQFGK"
     gene            5919..6830
                     /locus_tag="VN041_07970"
     CDS             5919..6830
                     /locus_tag="VN041_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU29012.1"
                     /translation="MTITTAAKRRRGSLAKVEARQALGFASPALVGLALFTIVPVGLS
                     IVMSFFDWPTFGARTFNGGANYLRLFEDPNFLPALRNSMVFTVLYVPLSVIFSLLLAV
                     GLGPRIRGRGALRVLFFIPVVTPMVANVLVWKMLLQPQGLFNGLAQTWFGVELPNFLA
                     DRNWAMIMVVVMSVWQGIGYNMLIFSAALEQLPESVLEAASIDGATGFRMTWSIMIPM
                     ISPAIFFATIMTMITSLQVFVQPQMLTGGGPGNSTLPLVMWIYNQGFKFQDLGLAAAG
                     AWILFALIITITAIQFTAQKRWVHYEH"
     gene            6820..7749
                     /locus_tag="VN041_07975"
     CDS             6820..7749
                     /locus_tag="VN041_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU29013.1"
                     /translation="MSTDIAVPSTKAMVAVDGDDHVDTERRSGDAPMTPAARVRLILA
                     HVAVYIAAALFMLPLVYAFFSALKPNGEMFAMPPSLFGSEIRWSNFAEVFQFGPFWTY
                     IGNSLFTSIAGTLVVLFVSTTAGYAFGRLRWKGRDAVFVLFLATLMVPAEVLVIPMFQ
                     VMQWFGWVDTYQALIFPFAFGAFGTFLMRQFFRGIPYELEEAARVDGAGPVRTFAQII
                     LPLSKSSIAVLSVFTFLSFWNSYLWPLIVTVDYGSLGTLPVGLASFSGLTGTRWDLQM
                     AASIISMIPTTILVIALQKHLVKGIAMAGLGGR"
     gene            7758..8795
                     /locus_tag="VN041_07980"
     CDS             7758..8795
                     /locus_tag="VN041_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU29014.1"
                     /translation="MATSLGTAMPFRRSLAPDGVIAGIDIGGTKIAALLVAADGEILG
                     RGQVAAPALDGGAAMADAAAELTRRLLEESGRPLLAAGVGAAGVIDHVTGTVRAASAT
                     FVGWAGFALADELRARLGVPVAVENDVNAFLLGEVSHGETTGDDVLGVMLGTGVGGAV
                     VLDGRLRHGPHGAAGEIGHTPGYSTLVCTCGQTGHLETLASGTSIGLRYGEMTGVAGL
                     SSPEVADRARAGDASAIAVFEAAGRAVALACASAAGLLDVSMAVVGGGVARAWDLLAP
                     AIERTLATDSPISGVTLRITSATLHTDAVALGAAAAAKAILLSGAAPSPHDPQSPHHS
                     SSPHHEEGAVA"
     gene            8792..>9897
                     /locus_tag="VN041_07985"
     CDS             8792..>9897
                     /locus_tag="VN041_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HWU29015.1"
                     /translation="MTDRLEAHEIWMPALAPVSPGGLERPRIGIGGISIESSTFSPHV
                     SGDEAFTIRTGADLIGYYPFLEAGRDLAEAAAWVPIHHGRSLPGGAVAPETYQRMKSA
                     IVDGIRESIRESGPFDGFFFDIHGAMSVIGMTDAEGDLAIAVREALGPDTLVSTSMDL
                     HGNVSETLRDAVDLLTCYRMAPHEDWMNTKERAVWNLLARLRSAIGSDPLRRRPFKAW
                     VPVPVLLPGEKTSTRLEPAAGLYAMLPEIEGREGVVDASVWIGYAWADEPRCQAYVVV
                     LGDDEQLIAREAERVARAFWDAREDFVFVAPTATLDGALERATAPGAQHPYLISDSGD
                     NPTAGGAGDVTWTLTELLGRPELTDGSRTTLVAS"
BASE COUNT         1557 a         3290 c         3435 g         1615 t
ORIGIN      
        1 ccgcgagtgc gccgagtccg agctgggccg gcagctgcca gccgcggaga agtacgcggc
       61 tcggcggcgc ggcggttccg gaaccgatcc gcgcataccg cgcgcgaccg cgcgcacgac
      121 tctccagcac cagcatcagc aggcacagca gcgtgaggac tccggcgagc atgcttccgg
      181 cggagccgtt gaacgtggac cggtactggt ccatgatcgc cgtcgtgaac gtgtcgaagc
      241 ggatcagcgc gaacgcgccg tactcgctga gcagatgcag gcccacgagc agcgatccgc
      301 cgagcagagc cagccgcaac tgggggagca ccgtgccgaa gaacacccgc acgggcccgc
      361 ggcccaggct cgcagcgctc tgctccagtg cgggatccag acccgccagc gccgcccgta
      421 caggcagata caccaggggg aagtacgaca gcgtggagag caggacaccc gagccgaggc
      481 cggcgagcga ggggatcgcc gacacccatg cgtaggagtt gacgaaggcg gggatcgcga
      541 gcggcatcgc gaggaccgcg ccccagaagc cggcgccggc gagtgaggtg cgctccacca
      601 gccatgctcc gcccacgccg agcacggcgg acagcggaac ggccagggcg acgagcagca
      661 ccgtgttgcc gagaagctcg ccgacccggg ggcggaagat cagagcgatc gactgatccc
      721 agccgatctg caccgccgtg gtgatcacga agatcagggg aagcagcgag agcgcggcga
      781 cgacgacacc ggcgaggacg acgccgaagg gcgggcggag cactcgggct ccgcccgccc
      841 tgacaggagc ggtggttgtc gtgatgttcc cgactttaga gcaggccggc ctgcgtcatc
      901 aggtcggtga ccttcttgga gttcagggtc gcgggatcga tcttcggggc gtccaggtcg
      961 gtcagcggca cgagctcgga gttcgcgggc acgtcggagc cgaccgggta ctcgaaggaa
     1021 gttccgttct gcagcacctc ctggcccttc ttgccggtga tgaaggccag gaacttctgc
     1081 gccgctgcct tgtgcttggt ggacttgagc acgccaccgc cggagatcga gacgaacgcg
     1141 cccgggtcct ggttcttgaa gtagtgcagg gcgacgttct tggagttctc gccggtctgc
     1201 gcctggtcgc cgaaccagta gtagtggtag atgatcgcgc cgtcgatctc gccggcgttg
     1261 accgccttca tcgcggtgga gttgcccttg taggcgacgg agttggtctt catcgcctgc
     1321 agccagctct cggctgcctt ctcgcccttg agctggacga gtgcggagac gatggcttgg
     1381 aagtcggcgc cggagggtga agctgcccag cggcccttcc acgccgggtc ggcgaggtcc
     1441 atcatcgact tcggcagctg cgcctcggtg agcttggtgg tgttgtaggc gaacacggtg
     1501 gagcgggctg cgatgccgac ccacttctcg gacgacgggc ggtactgctc ggggacgttc
     1561 ttcagcgcct cctcaccggg ggaggtgaac aggcccgcct tctcgaccgc ggtcatcgac
     1621 ggggagttct cggtcaggaa gacgtccgcg ggggagcggt cgccctcctg gatgatctgg
     1681 ttcgacatct cggtgtcgtc gccgttgcgg acctgcacct cgatgccggt ctccttctcg
     1741 aaggcgtcga tccactcctt ggtgagcgtc tcgtgctgcg cgttgtagac ggtgatcgtc
     1801 tcgtctgagg gcttggtggt ggacgacccg ctgtcggtgg cggcaggtcc gctgcacgcg
     1861 gcgagagtca gggcggcgac ggcggcggcc gcggtggctg agagcaggcg aagacgacgg
     1921 ttcacagtgg tccttcgatg aggggagcgg cgcgagtgcg cacctacaaa tataggtaag
     1981 gcaaccctaa tgctgcaatg tgatgagact cttccggact gatcagcgaa agacatccga
     2041 tgtctgtgtc aggatggacc catgcgacag gaatggttcg acgaggccag gttcgggatg
     2101 ttcgtgcact tcggcatcta cagcgttgcc gcgaggcacg agtgggtgca gagctacgag
     2161 cggctcacgg acgacgacta ccgccagtac ttcgagcact tcgatcccga tcgtttcgat
     2221 gcgcgcgcca tcgcccgcca ggccagggag gcgggtatgg gatacgtggt gctcacgacc
     2281 aagcatcacg acggcttctg cctgttcgac tcggctctga ccgacttcag ctcgcagcag
     2341 acgtcgggtc gcgacctcgt gcgcgagttc gtcgacgccg tgcgggcgga ggggctgaag
     2401 gtgggcctgt accactccgt tatcgactgg catcacccgg acttcaccgt ggactggaac
     2461 cacccgcgcc gcgacgacga gaacgcggca gccctcaacg agggccggga catggggcgg
     2521 taccgcgagt acctgcacgg tcaggtgcag gagctgctga cgcagtacgg ccggatcgac
     2581 tatctgttct tcgacttcac ctatcccgag agccgtgacg gctgggccgg caagggtccg
     2641 gatgcgtggg atgccgaggc gctgctgggc atgtgccgcg agctgcagcc cgacatgctc
     2701 gtgaacgacc gtctcggcat ccccgccgac ttcgtcaccc cggagcagta ccagcccacc
     2761 gcaccgctcg tcgacgagca cggggcgccg cgggtgtggg aggcctgcca gacgctgaac
     2821 gggtcgtggg gctatcaccg cgacaacacg gatcagaagt cgccgacgct gctggtgcag
     2881 atgctcgccg actcggtgtc gatggacggc aacatgctgc tgaacatcgg cccggacggg
     2941 cgcggcgcga tcgccccgcg cgacgcgcgc acgctggagg agatcggacg gtggatgctg
     3001 ctgcaccgtg acgcgatcgt cggcgccggg cacgcggagt tcacgccgcc ccgggaaggc
     3061 gtctacaccc tccgtggcga ccggctgtac ctgcacctgt tcagctggcc tctcggcttc
     3121 gtgcacctgc ccgatctggc cggtcgggtg tccttcgccc gcctgctcaa cgacgggtcc
     3181 tggctgaaga cctccgtgcc ggatcccgat cagcacgaca cccacatgac gccgaccctg
     3241 caggacgagg ggacgctgac ggtgcacctc ccggtgcagc ggccggacgt gctgatgccg
     3301 gtgatcgagc tgacgctcgc ggactgagag ggccctgagg ctgtcaggcc acgggcatct
     3361 ccaggtgatc catcgccagc ctcgccgcgc ccagcaggat cgcgtcggcg cccgtgcggg
     3421 cggcctcgat gcggagcgtc tgcgtgacga gcgggtggca cgcctcgtac acccggctgc
     3481 gcaccgcggc gaggaacggc tccgagccgc tcatgctgcc ggtgaggaag acggcgtgcg
     3541 ggttgaagaa gttgaccacg cccgacagcg cctgcccgag gtgtgtgccc gcggtgcgca
     3601 ccagggtcgt ggcctccgga tccgcgtccc gagcgagggc gaggacgtcc tccgtggtgt
     3661 gcaccgcctg gtgtcctcgt tcgcgcatct gacgcaccag gctggcaccg ctcgcgacgg
     3721 tctccagaca tcccgtgttc ccgcaggagc acgggatgtc tccgctgccg tcgatgcgcg
     3781 tgtgcgtgat gtctcctgct gctccggtcg cgccgcggtg cactcggccg tccacgatga
     3841 tcccgctgcc gatcgcggtg ccggccttga ccgtgatgct gtggccgtcg ccgccgagct
     3901 gggaggtgtg ctcgccgagt gccgcgaggt tcgcgtcgtt gtcgacgatc acgggtacct
     3961 cgtggcgcgc ggtcaggtgc tcgcccacgc ggaatccggg ccaacccggc atccgtgacg
     4021 gctgctcgac cgaaccggtc tccacgacga cgggaccggg cagtccgacg ccgatcgcac
     4081 gcacggacgc gttctcggcg aatcggtcga agacctcgct gacgcgctcg agtgtgcgct
     4141 tcggtccctc ggagagatcg atggcgatgg tctcgatgcg gtccagtgcg ccgccgaggg
     4201 tgtgcctgcc gatccgtgcg tgaccgccgc cgaggtcggc ggtcaggatg gtcccggcct
     4261 cggcgagctg gaggacgcgg ggcctgcggc ctccgcgtga cgtcccttcg ccggcctccc
     4321 gcaggatgcc ggcgtcgagc agggactgga cgcggatccc caccgtggac ggggccacgc
     4381 cgagcgcggt ggcgatctcg gtgcgcgaac gcgccgctcc ggtcgcgatc agctcgacga
     4441 tacggcgggt gggtgcgtcg tctgggctcg gcatgcctgt ctcccggggg ctctccgctg
     4501 cgtgatgcgc ggactaaaca tcggatgttc gtgttgcgtg tgtgctcgaa gtatgaaagc
     4561 gtaacttcgt tcaagacgtt accaggttgt ttcgatggtc gaaacaacct ttgatgagga
     4621 ggcaatcaat gaagatcaag cgcgtgggtg cggtgatcgt ggtcgcgggc gtcgccgtcg
     4681 ccatggcggg ctgctccaac ggcccgagca cgggcggctc cggcgacggg gacgcgatcg
     4741 tcgtcgacat gtgggcggga agcgcggatg acaccgctgc cctcaaggcg cagatcgacg
     4801 tcgtcaagaa gcagaacccc gacctgaagg tggagctgcg cacggctccc tggggcgact
     4861 tcttcacgaa gctgaccacg aacatggcat cgggcaacat ggcgtgcgtc accggcatga
     4921 actccggcat gctcgccagc tacaccgacg gcttcgtcaa gctgacggat gccgatctga
     4981 aggccgccgg actcgccgag agcgacttcg ccgcgggtgc gaccgacatc ctcaagaaca
     5041 agggcgacct ctacggcctg ccgttcgacg tgtccacgat ggtcacgtac tacaacgagg
     5101 accagctgaa cgcagccgga gccgagctcc cgaaggtcgg ctggaccttc gacgacttcg
     5161 aggcgacggc caagaaggcg accaccggcg gcaagttcgg tttcggcatc ggcatgggcg
     5221 acttccagtg gcaggcgctg cccatcgcca agtcgggcac gcagccggtc agcgcggacg
     5281 gcaagctcca gctcaccgac cccggattcg ctgccgctgc aggctggtac gcggatctgg
     5341 tcacgaagca gaaggtcgcc gctcccgtcg catccgcctc ggacggcgga tggggcgaga
     5401 accagttcac cggtgagaac gccgcgatgg cggtcgacgg cacgtggaac gcggtcggct
     5461 acctgaagaa cgacgcgggc ttcaaggcag gtctcgctcc tcttccgagc ggcgacaagg
     5521 gggatctgag cctggtgctc ggctccggct tcggcatcgc gaagacctgc aagaacaagg
     5581 acgccgcgct gaagatgctc ggttcgctgc tgagcaagga ctcgcaggac tacatcgcgt
     5641 cctcgggccg cagctacccg gctcgcgcgg agagccagcc gctctacttc gagtcgctcg
     5701 acccggcgta ccgcgaccag gtcaaggccg tcttcgaagc ggcgttcaag aacgtcgagg
     5761 gtcagcacgt ctccgacaac tgggccaagg tcggcacctt cgtgcagccc gagctggtga
     5821 gcgtctacaa cgggcaggag tcgatgaagt cggttctgga gtccgcgcag cagcagttcg
     5881 gcaagtgacc cggaactgac ggaaagggat accaggagat gaccatcacg accgctgcca
     5941 agcgtcgtcg cgggtcgctc gccaaggtcg aggcgcgcca ggcgctgggc ttcgcaagcc
     6001 cggccctggt gggcctcgcc ctgttcacga tcgttccggt cggcttgtcc atcgtgatga
     6061 gcttcttcga ctggccgacc ttcggcgcgc gcaccttcaa cgggggagcg aactacctcc
     6121 ggctgttcga ggacccgaac ttcctccccg cgctgcggaa ctcgatggtg ttcaccgtcc
     6181 tgtacgtgcc gctgagcgtg atcttctcgc ttctgctggc ggtcggtctg ggcccccgca
     6241 tccgcgggcg cggtgcgctg cgggtgctct tcttcatccc ggtggtgacg cccatggtcg
     6301 ccaacgtcct cgtctggaag atgctgctgc agccgcaggg cctgttcaac ggcctcgcgc
     6361 agacctggtt cggcgtggag ctgcccaact tcctcgctga ccggaactgg gcgatgatca
     6421 tggtcgtcgt catgagcgtc tggcagggca tcggctacaa catgttgatc ttctccgcgg
     6481 ctctcgagca gctgccggag agcgtgctgg aggcggccag catcgacggc gccaccggct
     6541 tcaggatgac ctggagcatc atgatcccga tgatctcccc ggcgatcttc ttcgccacca
     6601 tcatgacgat gatcacgtcg ttgcaggtgt tcgtgcagcc gcagatgctg acaggcggag
     6661 gcccgggcaa cagcacgctg cccctcgtga tgtggatcta caaccagggc ttcaagttcc
     6721 aggacctcgg cctcgccgcc gcaggcgcct ggatcctgtt cgccctcatc atcacgatca
     6781 ccgccatcca gttcacggcg cagaagaggt gggtgcacta tgagcactga catcgcagtc
     6841 cccagcacga aggcgatggt cgcggtggac ggcgacgatc acgtcgacac cgagcgccgc
     6901 tccggcgacg cgccgatgac gccggccgcg cgcgtgcgcc tcatcctcgc gcatgtcgcg
     6961 gtctacatcg ctgccgccct gttcatgctg ccgctggtct acgcgttctt ctcggccctc
     7021 aagccgaacg gcgagatgtt cgcgatgccg ccgagcctgt tcggatccga gatcaggtgg
     7081 tcgaacttcg ccgaggtgtt ccagttcggg ccgttctgga cgtacatcgg caactcgctg
     7141 ttcacgtcga tcgcgggaac cctggtggtg ctgttcgtct ccaccaccgc cggctacgcg
     7201 ttcggcagac tgcggtggaa gggacgcgac gccgtcttcg tgctcttcct cgccactctc
     7261 atggtccccg ccgaggtgct ggtcatcccg atgttccagg tgatgcagtg gttcggatgg
     7321 gtggacacgt accaggcact gatcttcccg ttcgcgttcg gcgcgttcgg cacgttcctc
     7381 atgcggcagt tcttccgcgg catcccgtac gagctggagg aggcggcccg tgtcgacggc
     7441 gcggggccgg tgcgcacctt cgcgcagatc atcctgccgc tgtccaagtc gtcgatcgcc
     7501 gtcctgtcgg tgttcacgtt cctgtcgttc tggaacagct acctgtggcc cctgatcgtc
     7561 acggtggact acggctccct cggaacccta cccgtcggcc tcgcgagctt ctccgggctc
     7621 accggcacgc gctgggacct gcagatggcc gcttcgatca tctcgatgat cccgaccacg
     7681 atcctggtga tcgctttgca gaagcacctg gtcaagggca tcgccatggc cggcctcggg
     7741 gggcgttgaa cggatcgatg gccacgtcac tcggcacagc catgcccttc cgaaggtcgc
     7801 tcgcgcctga cggcgtgatc gccggcatcg acatcggagg gaccaagatc gcagcactgc
     7861 tcgtggctgc ggacggggag atcctcggtc gcggacaagt ggccgctccc gcgctggacg
     7921 ggggagcggc catggcggac gcggccgcgg agctcacccg ccgcctcctc gaggagagcg
     7981 gacggcccct gctcgccgca ggagtcggcg ctgcgggcgt gatcgatcac gtcacgggaa
     8041 ccgtgcgcgc cgcatcggcc accttcgtcg gctgggcggg tttcgcgctc gcggatgagc
     8101 tgcgcgcgcg tctcggcgtg cccgtcgcgg tggagaacga cgtgaacgcg ttcctgctcg
     8161 gcgaggtcag tcacggcgag accacgggag acgacgtgct cggcgtgatg ctgggcacgg
     8221 gcgtcggcgg cgcggtcgtg ctggacgggc gtctgcgcca cggaccgcac ggagcagcgg
     8281 gcgagatcgg gcacacgccg gggtacagca cgctcgtatg cacctgcgga cagacgggcc
     8341 acctggagac gctcgcctcg ggcacctcga tcgggctgcg atacggcgag atgaccgggg
     8401 tcgccgggct cagttccccg gaggtcgcgg acagggcccg cgcgggtgac gcgagcgcga
     8461 tcgcagtgtt cgaagcggca gggcgagcgg tcgcactcgc ctgcgccagc gcagccgggc
     8521 tgctcgacgt gtccatggcc gtcgtcgggg gaggcgtggc ccgcgcgtgg gatcttctcg
     8581 cacccgccat cgagcgcacg ctcgcgacgg attcgccgat ctccggcgtc accctgcgga
     8641 tcacatccgc gaccctgcac acggacgccg tggcgctcgg agccgccgcc gcggcgaagg
     8701 ccatcctgct ctcgggcgcc gcaccgagcc cccatgatcc acagagtccc caccactcat
     8761 cgagccccca ccacgaggaa ggagccgtcg catgacggac cgtctggaag cccacgagat
     8821 ctggatgccc gcgctggcgc ccgtgtcgcc cggcgggctg gagcgaccgc gcatcggcat
     8881 cggcggcatc tccatcgagt cgagcacgtt ctcaccgcat gtctcaggag acgaggcgtt
     8941 cacgatccgg accggcgcgg acctcatcgg gtactacccg ttcctcgaag caggacgcga
     9001 ccttgctgag gccgccgcgt gggtcccgat ccaccacggc cgttcgcttc ccggcggggc
     9061 tgtcgcgccg gagacctatc agcggatgaa gagcgccatc gtcgacggca tccgcgagag
     9121 catccgcgag tccggaccct tcgacggctt cttcttcgac atccacggcg cgatgagcgt
     9181 gatcggcatg accgacgccg agggcgacct cgcgatcgcc gtgcgcgagg cgctggggcc
     9241 ggacacgctc gtctccacgt cgatggacct gcatggcaac gtctccgaga ctctgcgcga
     9301 cgccgtcgat ctgctcacct gctatcgcat ggcgccgcac gaggactgga tgaacaccaa
     9361 ggagcgggcg gtgtggaatc tgctcgcgcg gctccgcagc gcgatcggct ccgacccgct
     9421 gcgacgtcgt cccttcaagg cctgggttcc cgtccccgtg ctccttccgg gcgagaagac
     9481 gagcacccgt ctggagcccg cggccggcct ctacgcgatg ctgccggaga tcgaaggcag
     9541 ggagggcgtc gtcgacgcct ccgtctggat cggatacgcg tgggcggacg aaccgcgctg
     9601 ccaggcctac gtcgtcgtgc tcggcgatga cgagcagctc atcgcgcgcg aagccgagcg
     9661 cgtggcgcgg gccttctggg acgcgcgcga ggacttcgtg ttcgtcgcgc ccaccgcgac
     9721 cctcgacgga gcgctcgagc gcgcgacggc gccgggggcg cagcatccgt atctgatctc
     9781 cgactccggg gacaacccga ccgccggcgg tgcgggcgac gtcacctgga cgctgaccga
     9841 gctgctcgga cgtccggagc tcacggacgg cagccgcacg accctggtcg cgtcgat
//
LOCUS       DATMSX010000264         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_108219,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000264 DATMSX010000000
VERSION     DATMSX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108219"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VN041_07990"
     CDS             <1..406
                     /locus_tag="VN041_07990"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29016.1"
                     /translation="TLAARAALDALGLRSVGLVFPGPRGIAEEIVAQYEADGIRMPVL
                     SAPEVELANAEIARVSDDWIDALMRPAFSGEVDGVLCIGTNLRSAYRVAQFEAEFGIP
                     VIDSATATLWHLLQLAGAARPVPGWGSLLARG"
     gene            complement(424..1317)
                     /locus_tag="VN041_07995"
     CDS             complement(424..1317)
                     /locus_tag="VN041_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWU29017.1"
                     /translation="MTAHPLPRDLRGYGEHPPEVLWPGGSRAAVSLVINVEDGSERSR
                     ARGDDADDLGAHWTAHPAHPHQRNLDLESAFEYGARAGIWRLLRTLRRHDARATAFCC
                     AAGLEANPRIAEALVRDGHEIADHGLLWEPHTLLTPEDEHARMVRSREIIEALTGRRP
                     TTWYSRDGLTEHSRSSMIAEGFRYDANSFNDDVPHAGPDGLALPVIPYAGDTNDSALL
                     RQYPTAASFAGHLCDALDMLLDDPREGAGVLSVGLHPRLIGRPAYIGALDALLRRARE
                     KGAWIATREQIAEHWSRAIAA"
     gene            1398..>1827
                     /locus_tag="VN041_08000"
     CDS             1398..>1827
                     /locus_tag="VN041_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29018.1"
                     /translation="MPTLIRAGVVVPATGGTILEGGAVVVDDGRIVEVLDALGARRAA
                     EALAGEVVDEPDSILMPGLVNIHTHGVSSSPLFPSGSAPLPDERWLGHLDRHAVAGTT
                     TVLSTCGLGTMDEIREADRRHAVHVRGATAHTPGSIAAALA"
BASE COUNT          248 a          618 c          693 g          268 t
ORIGIN      
        1 gacgctcgcc gcgcgcgccg cgctggacgc gctcgggctg aggtcggtcg gtctggtgtt
       61 cccggggccg cgtggcatcg ccgaggagat cgtcgcgcag tacgaggccg acggcatccg
      121 gatgcccgtg ctgtcggcac ccgaggtcga actggccaac gccgagatcg cccgcgtcag
      181 cgacgactgg atcgatgccc tgatgcgacc ggctttctcc ggcgaggtcg acggcgtgct
      241 gtgcatcggc accaacctgc gcagcgcgta ccgcgtggcg cagttcgaag cggagttcgg
      301 gatcccggtg atcgacagcg cgacggcgac gctctggcac ctgctgcagc tcgccggtgc
      361 ggctcgcccc gtcccggggt gggggagcct gctcgcacga ggctgagcga ggcccatcgc
      421 gtctcaggcg gcgatcgccc tgctccagtg ctcggcgatc tgctcgcggg tggcgatcca
      481 ggcacccttc tccctggcgc ggcggagcag cgcgtcgagg gcgccgatgt acgcagggcg
      541 cccgatcaga cggggatgca ggcccacgct gagcacgcct gcgccctcgc ggggatcgtc
      601 gagcagcatg tccagcgcat cgcacagatg ccccgcgaag gatgctgccg tcgggtactg
      661 gcgcagcagg gcggagtcgt tggtgtcgcc cgcgtagggg atgaccggaa gggccagtcc
      721 gtccggcccc gcgtgcggga cgtcgtcgtt gaagctgttc gcgtcatagc ggaatccctc
      781 ggcgatcatc gacgagcggg agtgctcggt gaggccgtca cgggagtacc aggtcgtcgg
      841 ccgccggccg gtgagcgcct cgatgatctc gcgggagcgc accatgcgcg cgtgctcgtc
      901 ctcgggggtg agcagggtgt gcggctccca gagcaggccg tggtcggcga tctcgtgccc
      961 gtccctgacg agggcctcgg cgatgcgggg attggcctcc agcccggcgg cgcagcagaa
     1021 cgctgtggca cgggcgtcgt gacgacgcag ggtgcgaagc aaccgccaga tccccgcgcg
     1081 ggcgccgtac tcgaaggcgg actcgaggtc gagattgcgc tgatgcggat gcgccggatg
     1141 agccgtccag tgcgctccga gatcgtcggc gtcatcgccc cgggcgcgac tgcgctcgga
     1201 gccgtcctcg acgttgatga ccagggagac cgcggcacgg gagccgcccg gccagagcac
     1261 ctcgggcggg tgctcgccgt agccgcgcag gtctcgggga agggggtgcg cggtcatcgg
     1321 tcctcctggc tatagtgaag cggcttcgat atgatgaatc taccggtgac gaggccggcc
     1381 gtcgagagga ggagtccatg ccgactctta tccgcgccgg cgtggtggtg cccgccaccg
     1441 gcgggacgat cctcgagggc ggcgccgtgg tcgtcgacga cggccggatc gtggaggtgc
     1501 tggatgcgct gggcgcgcgt cgcgccgcgg aggcgctcgc cggcgaggtc gtcgacgagc
     1561 ccgacagcat cctcatgccc ggactggtca acatccacac gcacggcgtc agttcgtcgc
     1621 cgctgttccc cagcggctcc gcaccgctgc cggacgagcg ctggctcggc cacctcgacc
     1681 gtcatgcggt cgccgggacg acgaccgtgc tcagcacctg cgggctcgga accatggacg
     1741 agatccgcga ggcggaccgt cgtcatgccg tgcatgtgcg cggcgccacg gcgcacaccc
     1801 ccggttcgat cgcggcggcg ctcgccg
//
LOCUS       DATMSX010000265        12816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_136434,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000265 DATMSX010000000
VERSION     DATMSX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136434"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VN041_08005"
     CDS             <1..285
                     /locus_tag="VN041_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29019.1"
                     /translation="LEADARMPEAPAPQAAVQQASAQTASAQPASGQLWAEASDLLDG
                     LDDASTGPGSRRGFDPVELTVTLTADEHARLRDRADAQGVSLQEALRRLI"
     gene            305..601
                     /locus_tag="VN041_08010"
     CDS             305..601
                     /locus_tag="VN041_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWU29020.1"
                     /translation="MWIGWVEFDILLGDVHSLKQKRSVIRPIIADLARRTEAAVAEVG
                     AQDLHRRTMIGVSVVATHAGHVRDVLDQAERMLVDQHPEVTLLSARRGLRSSED"
     gene            626..1381
                     /locus_tag="VN041_08015"
     CDS             626..1381
                     /locus_tag="VN041_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HWU29021.1"
                     /translation="MTEASSPDRAAHDEWRRQRVSAATAPTGKLSLVETRWFPSGTSE
                     AEAAALTGAERASAVGRVTVSTLDRENATTGEPEFGLRVWDADAPAIGAFEGIDTFPF
                     DPAWVVEGEFRPASGQVVAFEHIRDAGSTRDHAIPGEVVFTREGQTFTLAALDNGDTL
                     LVVFGDPTNGVGGEDGTYGPGRFLFLPLDESGETGVTRPIRIDFNRAAVPPCGFSAQF
                     NCPLPPMQNRLPWPVRAGEKNVRFRAGFDMYSA"
     gene            complement(1460..2452)
                     /locus_tag="VN041_08020"
     CDS             complement(1460..2452)
                     /locus_tag="VN041_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU29022.1"
                     /translation="MPVIDINADTENLTLTVTAEFAAPIERVWYAYSDPRQLERFWGP
                     PSWPATFLTWDHTVGGRADYAMTGPHGERHRAYWEFLEIEEPNRFSVLDGFSDDEGKP
                     NGDLPAMRMDFTFEPTTDGTRMVTSSRFDSLEALEQVIAMGVIEGTRMAMSQIDAVLR
                     DLREYAEGKGTTVEFIDDTHISITRLVNGPRDLVWRAYTEPDLIKQWMLGPDGWEMTE
                     CEVGLKAGDRYRNSWAPVGDNEGEPFGFEGEMLVVEAPRRAVQSERMQGTDGPDILSD
                     VSFYEEDGATLITVIMEFPDKDTRDGILATGMTDGMEASYQRLESTVLAGESVA"
     gene            complement(2454..2864)
                     /locus_tag="VN041_08025"
     CDS             complement(2454..2864)
                     /locus_tag="VN041_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU29023.1"
                     /translation="MVVQSELSEAEIDRVFHALATATRRDILRRTIEREQSVSDLARD
                     YEMSFAAVQKHVAVLEEARLIVKRAEGRERLVRANPEMIARARALLARYEELWRARIA
                     RLDDLLAGPPPSEAVPPVSDPTDKNHDTDKNQGA"
     gene            complement(3018..4205)
                     /locus_tag="VN041_08030"
     CDS             complement(3018..4205)
                     /locus_tag="VN041_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWU29024.1"
                     /translation="MMNNEPNGTQDTTALLADDERVTPFVLSIPDADLNDLRDRLART
                     RYTTEVPGADPAYGASLTAVRAMAEYWRDEFDWRSVEAKLAAFPQFMTTIDGQPIHFL
                     HVRSAEPEAFPIVLTHGWPGSFAEFADMIGPLVDPVAHGGRAEDAFHVVVPSFPGYAF
                     SGPVTEPGWGSARVAGAWDTLMKRLGYERYGAHGGDGGSLVGRELGILAPDGLVGTHV
                     LQLFSFPSGDPAEFEKLGPKDYEALEILKDFGEISAFATLQSTRPQTLAHALADSPVG
                     QLAWNELMATFDRPTPLTRDQILTHVSLYWFTNTAGSSARQYKEDARFNAGKGISARV
                     NHAPTGVSVFRNDFHSIRAFAERDNDRIVFWKEHEVGGHFAAMEVPDVLVADLREFFG
                     PLR"
     gene            4264..5220
                     /locus_tag="VN041_08035"
     CDS             4264..5220
                     /locus_tag="VN041_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HWU29025.1"
                     /translation="MNQTATRVLQVLALLRQRRAWTASELAQRTSVSTRTIRADIARL
                     RELGYVIDATAGPDGGYRLDRETDLPPLMLDDDEGIAVAIALHQVTTNSVAGIEGSAA
                     RALAKLENVLPPALAAQVRAMDVATAPQTRAADAEVEASVLAAIARAVQDAEWLRFEY
                     TPFQGERASRRVEPYRLVSWGRRWYLVAFDLERDDWRSFRVDRMSLRPPTRRTFTPRD
                     LPADDVAGYVLRGVASAGWRFRARVLIHAPASAVADKIDPSVGLVEAVDDARCVLATG
                     ADHPMTVAVHLALLDEPFTVDGPPELSDAIRTLARRYAEATA"
     gene            complement(5236..6300)
                     /locus_tag="VN041_08040"
     CDS             complement(5236..6300)
                     /locus_tag="VN041_08040"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWU29026.1"
                     /translation="MTLRGIARRRLRSQLLEAPAADAETVVRTLLAVQAENPAQSAWA
                     VAARTAAPDREDLAIALTEGRIVRTHVLRPTWHYVHRDDARWVIELTAPRVLPVFDQQ
                     LRPSADRITELTDAIMRTVSGDTGRTRAEIAATLADAGLDLPASQLTLLLGRLELQCL
                     LSSGAPRDGQHTWALMDERMPPAAAVDRDEALARLAFRYLTAHGPATDRDLAYWSTLT
                     LTDARRGIAAVSARLECFDHDGRTYWHAPGDESPAGRGPAHLLQILDEMYRGYQDSRW
                     ALDSEGIVPREREASIGMVLCDGQLVAGMRRSVDARRVVFEVTPHRGLRLRERTEIQR
                     AARRCAAYLGVEPEVQFTSE"
     gene            complement(6297..6839)
                     /locus_tag="VN041_08045"
     CDS             complement(6297..6839)
                     /locus_tag="VN041_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29027.1"
                     /translation="MSTVALTKVALLIVVVALVPAVVWWMRRHPNRSKQHPDRQRMPK
                     LVAVVGWLLLVVGVLMSLVAFTSSDADDPTAFRIASVAILFGGVLFLMMYRNWYVAPE
                     RDAVHYRTLLGREDRIIYSDIVDYRMTEANGQPNLRIRASNGAKLALNPAMYDMSPLL
                     AAIRFKEARGRWPLPGELTG"
     gene            complement(6917..8236)
                     /gene="hisD"
                     /locus_tag="VN041_08050"
     CDS             complement(6917..8236)
                     /gene="hisD"
                     /locus_tag="VN041_08050"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWU29028.1"
                     /translation="MRYSSPVLQSLAGSFQHLKTPLIDAPAAQRDPAVIETVSAMLSD
                     IQKRGMDAVLDYARKLDRYDGGDIELSAQQIATSGDRLPADLRAAIELGAERTQAFAR
                     KQREHLADFETELVPGLVTGARYIPVSRVGAYLPAGRFPLTASAFMTVGVAKAAGVPT
                     VIACTPPQPDGGANDAVVYAAHLSGVDRVFVLGGVQALAAMAYGLLGDLPVDMLVGAG
                     NAFVAEAKRQLFGTVAIDLLAGPSEVAVISDETADPEIVAADLLGQAEHGPNSPAALV
                     TTSEEHGRAVIAAVDAQLETLATAEIAGAAWRDYGSVTVAKDRETAAALMDDLAPEHL
                     ELITADDEWYHNRLRNYGSLFIGPWSTVAYSDKGMAGTNHVLPTAGGAKHSAGLSVSR
                     FLKPLTYQRIAQPATPELANAVQVISDSEGMAAHSATATRRLALYAD"
     gene            8313..9239
                     /locus_tag="VN041_08055"
     CDS             8313..9239
                     /locus_tag="VN041_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU29029.1"
                     /translation="MTIAQLRAFLLALELGSFTAAASELGMTQASVSELIARLERELG
                     VPLFTRGGRRLVPTAAAVELRPHAEQAIGAIRNGVETVRSLTALEGGTCTFGVLRNAA
                     YYALADLAETFHYRHPNVRVRLTGINSALVARSIARGEVEAGLVVLPVEENGLSIRPL
                     FRDEVLYASAQRPASAGAVTIDETADAKLVLYDAFAGWDDPTRRQLLERARLRGLQLE
                     PAIEVEHVETALSLVASGAADTIVSRTIAESDAFPEGIRTFPFAEPMYDTIALVQREG
                     VVLSPATQRLAELAERMLLESVSEHGGRTRLV"
     gene            complement(9248..10042)
                     /locus_tag="VN041_08060"
     CDS             complement(9248..10042)
                     /locus_tag="VN041_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWU29030.1"
                     /translation="MTMLTVAAISPEVRIGDVDGNLARLADVIRDAVRRGAGLIVLPE
                     LATSGCMFTDRDEVLAAALRRDDPRWRLLQDALSESAVVVVGYAEDDDGGPYNTSAAL
                     TRDRRLHDYRKAHLWGAEVNLFTPGHEAGAVVDAPFGRLGLAVCYDDEFPEVPRRIAL
                     AGADVLALPVAWPAVPRPAGEHPPETIQAMGSARSSRMPIVIADHHGSERGVEWTGGT
                     AVVDGLGWLAAEDERFAMAELDLDSLRDKSLPPYNHLFTDRRPELY"
     gene            complement(10135..11283)
                     /locus_tag="VN041_08065"
     CDS             complement(10135..11283)
                     /locus_tag="VN041_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29031.1"
                     /translation="MNDVIVDAAEIGRRTIRKSDWVACNAAFIDCRTPGSDKKENYSF
                     IGVGVSQNASQYINLTEKHGFNTGAAGMPNGISNNLHLHFTAEVFVNLGGEFRLRWGV
                     DGKQGEYISHDGDIISIPTWIFRGFTNEGPDDGVLYTVLGRDDTGGIIWGPSVLREAE
                     SYGLHLTADNRLIDTVAGDELPEGVPLITPMKQHYIDELTSYSVEEMRSRVVQPGDRR
                     YGSHALLCTAVDGGRVDLALVIGYGMTQNRRQVPSIHEPHSFNLAWVRAEAGQGVLRY
                     RQQQTQALLIKSGQWEVVLNGDPATALRFGPGDSFSVPVGAWRSFTCVENGESGAGEV
                     LVINGGDDRVYPEWAPEVVEAAAAADATIDPNGYLAPLGVMVTATEDD"
     gene            complement(11330..12310)
                     /locus_tag="VN041_08070"
     CDS             complement(11330..12310)
                     /locus_tag="VN041_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWU29032.1"
                     /translation="MPLDPVAYQPYKDPDDFIREVTDLIWVDRSISYIRENYEPDSIV
                     HGAYGTSTSRAEVIEGTLMRISDTPDRTGQAEDVIWEARGDDAFLSSHLVLSGHLNEV
                     GVSRTIANCLYRRGRMVEEWVVRDSLAGALRSGADLDELARAQAFRGYLGSWTEAAPA
                     DPIAVGDSGARPDDYRSEVELVIDMINTVWNDRDLQKVERYFQRDLVLLTVGDRTIIR
                     PEGYRRALLRFLESFPAGQFEIRDIQTNYDVRYAGLRVAVTWKFTGDYNGRANYGPLT
                     GKPVDVLGISQFTFHQGALVKEVRLWDDIALRAQIAGMRGDEPVGPSNIY"
     gene            complement(12462..>12816)
                     /locus_tag="VN041_08075"
     CDS             complement(12462..>12816)
                     /locus_tag="VN041_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU29033.1"
                     /translation="YGAVEGEAAAAELLAGDEPPTVLFCGNDVVAYGALNAARRAGLS
                     VPGDVSIIGFDDLPEASWPIVDLTTIGYDITGMARAAADLIVRRIEHPDAERSEITFA
                     SSFVPRSTVAAPRPR"
BASE COUNT         1929 a         4364 c         4531 g         1992 t
ORIGIN      
        1 ctcgaggcgg atgcccggat gcctgaggcg cccgcgccgc aggccgccgt gcaacaggca
       61 tcggcgcaga cggcatccgc gcagccggca tccggacagc tctgggccga ggcatccgat
      121 ctcctcgacg gtctggacga cgcatcgacg ggtccgggaa gccggcgcgg gttcgacccg
      181 gtcgagctga cggtcaccct cacggccgac gagcacgcgc ggctgcgcga ccgcgcggat
      241 gcgcagggcg tctcgctgca ggaggcgctg cgccggctga tctgagcgat gtcagactga
      301 cagcatgtgg atcgggtggg tggagttcga catcctgctc ggcgacgtgc actcgctcaa
      361 gcagaagcgc agcgtgatca ggccgatcat cgcggatctc gcgcggcgca ccgaggcggc
      421 ggtggccgag gtcggcgcgc aggatctgca ccgccgcacc atgatcggcg tctccgtcgt
      481 cgccacgcac gccggtcacg ttcgcgatgt gctggatcag gccgagcgga tgctggtgga
      541 ccagcatccg gaggtcaccc tgctgtcggc gcgacgcgga ctgcgctcca gcgaggacta
      601 gggattccgc accagaatgg aacccgtgac cgaagcatcc tccccagacc gggccgcgca
      661 cgacgagtgg cgccggcagc gcgtctcggc cgcgacggcg cccaccggca agttgagcct
      721 ggtcgagacc cgctggttcc cctcgggaac gagcgaggcc gaggcggccg cgctcaccgg
      781 ggcagagcgc gcgagcgccg tcgggcgcgt cacggtgagc actctggatc gtgagaacgc
      841 gacgacgggc gaacccgagt tcgggctgcg tgtgtgggac gcggatgcgc cggcgatcgg
      901 tgctttcgag ggcatcgaca cgttcccctt cgaccccgcc tgggtggtcg agggcgagtt
      961 ccggccggcg tccgggcagg tcgtggcgtt cgagcacatt cgcgatgccg gatcgacccg
     1021 cgatcacgcg atcccaggtg aggtcgtgtt cacccgcgag ggacagacgt tcacgctggc
     1081 cgcgctcgac aacggcgaca cactgctggt ggtgttcggc gaccccacca acggcgtggg
     1141 cggcgaggac ggcacctacg ggcccggtcg gttcctgttc ctgccgctcg acgagtccgg
     1201 cgagaccgga gtcactcgac ccatccggat cgacttcaac cgtgcggcgg tcccgccgtg
     1261 cgggttctcg gcgcagttca actgcccgct gccacccatg cagaaccgcc tgccgtggcc
     1321 ggtgcgggcc ggcgagaaga acgtgcgctt ccgcgccggc ttcgacatgt acagcgcgta
     1381 gcgcggcatc cgctcgcaga gacgcacaac tccgcagccg gaccgcgatc cggttgcgga
     1441 gctgtgcaga actgcggagt tacgcgacgg actcgcccgc gagcaccgtc gactcgagac
     1501 gctggtacga cgcctccatg ccgtcggtca tgccggtggc gaggatcccg tcgcgggtgt
     1561 ccttgtcggg gaactccatg atcacggtga tcagggtcgc gccgtcctcc tcgtagaagc
     1621 tcacgtcgct gaggatgtcg gggccgtccg tgccctgcat ccgctcgctc tgcacggcac
     1681 ggcgcggggc ctcgacgacg agcatctcgc cctcgaaccc gaacggctcg ccctcgttgt
     1741 ctccgaccgg cgcccacgag ttgcggtagc ggtcgcctgc cttcaggccg acctcgcact
     1801 ccgtcatctc ccagccgtcg gggccgagca tccactgctt gatcaggtcg ggctcggtgt
     1861 aggcgcgcca gaccaggtcg cgcgggccgt tgacgagccg ggtgatgctg atgtgcgtgt
     1921 cgtcgatgaa ctcgacggtg gtgcccttgc cctcggcgta ctcgcgcagg tcgcgcagca
     1981 cggcgtcgat ctgcgacatg gccatgcggg tgccctcgat cacacccatg gcgatgacct
     2041 gctccagcgc ctcgagcgag tcgaaccggc tggaggtgac catgcgcgtg ccgtcggtgg
     2101 tcggctcgaa cgtgaagtcc atgcgcatcg cgggcaggtc gccgttgggc ttgccctcgt
     2161 cgtcgctgaa gccgtcgagc acgctgaacc ggttcggctc ctcgatctcg aggaactccc
     2221 agtaggcgcg gtgccgctcg ccgtgggggc cggtcatggc gtagtccgcg cgtccgccga
     2281 ccgtgtggtc ccaggtgagg aaggtggccg gccagctcgg cggaccccag aagcgctcga
     2341 gctgacgcgg gtcgctgtag gcgtaccaca cgcgctcgat gggggccgcg aactcggcgg
     2401 tgacggtgag ggtcaggttc tcggtgtcgg cgttgatgtc gatcacgggc atgtcatgct
     2461 ccttggttct tgtcggtgtc gtggttcttg tcggtgggat cggataccgg cggcaccgcc
     2521 tcgctcggag gaggaccggc cagcagatcg tcgagccggg cgatgcgtgc ccgccagagc
     2581 tcttcgtatc gggcgaggag cgcccgcgcg cgggcgatca tctcggggtt cgcgcggacg
     2641 agccgctcgc gtccctcggc gcgcttgacg atgagccgtg cctcttcgag cacggccacg
     2701 tgcttctgga ctgcggcgaa ggacatctcg tagtcgcggg ccaggtcgga aaccgactgc
     2761 tcgcgctcga tcgtccggcg caggatgtcg cgccgggtcg ccgtcgccag tgcatggaac
     2821 acacggtcga tctccgcttc gctgagttca ctttgtacaa ccatttggtt gtacgttatg
     2881 cgaggcgggc ggtggtgtca agagttttct gcggtcgcac gtggcggccg cgacggtgcc
     2941 tcagcgcggg tatccgcgct gaggcaccga agaccggtcg gctgcacctg ctctcagaag
     3001 cggccgtcgc gcatcgatca gcgcagtggt ccgaagaact cgcggaggtc cgcgacgagc
     3061 acgtccggca cctccatcgc ggcgaaatgc ccgccgacct catgctcctt ccagaacacg
     3121 atccggtcgt tgtcccgctc agcgaaagcg cggatcgagt ggaagtcgtt ccggaatacc
     3181 gacaccccgg tcggggcgtg attgacccgt gccgagatgc ccttacccgc gttgaaacgg
     3241 gcgtcttcct tgtattgccg cgcggatgag cccgcggtgt tggtgaacca gtacagcgac
     3301 acgtgcgtga ggatctggtc acgcgtcagc ggggtcggac gatcgaacgt cgccatgagc
     3361 tcgttccacg ccagctgacc gacgggcgag tcggcgagcg catgcgccaa ggtctgcggg
     3421 cgggtggact ggagggtcgc gaatgcggag atctcgccga agtccttcag gatctcgagc
     3481 gcctcgtagt ccttcgggcc gagcttctcg aactcggccg ggtcgccgct cgggaacgag
     3541 aacagctgca gcacgtgcgt gcccaccaac ccatcggggg cgaggatgcc gagctcacgg
     3601 ccgacaaggg agccgccgtc tccaccgtgc gcgccgtatc gctcgtagcc caggcgcttc
     3661 atgagcgtgt cccaggcccc ggcgaccctg gccgaccccc agccaggctc cgtcacggga
     3721 ccactgaacg cgtaaccggg gaagctgggc accacgacgt ggaaggcatc ctccgcacgc
     3781 ccgccgtgcg ccacgggatc caccagcggt ccgatcatgt cggcgaactc cgcgaacgac
     3841 cctggccagc cgtgggtgag cacgatcggg aacgcctcgg gctcggcgga gcgcacgtgc
     3901 aggaaatgga tcggctgacc gtcgatcgtc gtcatgaact ggggaaaggc ggccagcttc
     3961 gcctcgacgc tgcgccagtc gaactcgtcg cgccagtact cagccatcgc gcgcaccgcg
     4021 gtgaggctgg cgccgtacgc gggatccgcg ccgggcacct cggtcgtgta ccgggtgcgg
     4081 gcgaggcggt cgcggaggtc gttgaggtcg gcgtcgggaa tcgacagcac gaatggggtt
     4141 acccgctcgt cgtcggcgag gagtgcggtg gtgtcctggg tgccgttcgg ctcgttgttc
     4201 atcatggctc taagttagga actgatgcgg aaggatagct tccgcatgga aggcggtgag
     4261 cgagtgaatc agaccgcgac acgcgtgctg caggtgctgg cgctgctgcg tcagcggcgg
     4321 gcctggaccg cctccgaact ggcgcagcgc acgtccgtgt cgacgcggac gatccgcgcc
     4381 gacatcgcgc ggcttcgcga gctcggctac gtgatcgacg ccacggcggg gccggacggc
     4441 ggctaccggt tggaccggga gaccgacctt ccgccactca tgctggacga cgacgagggc
     4501 atcgcggtcg cgatcgccct gcaccaggtg acgacgaact ctgtggcggg catcgagggc
     4561 agcgcggcgc gggcgctggc gaagctcgag aatgtgctgc ccccggcgct cgccgctcag
     4621 gtgagagcca tggacgtcgc gaccgcgccg cagacgcggg ctgcggatgc cgaggtcgag
     4681 gcatccgtgc tggcggccat cgcccgggcg gtgcaggacg ccgaatggct gaggttcgag
     4741 tacacaccgt tccagggtga gcgcgcctcg cgccgcgtcg agccgtaccg gctggtcagc
     4801 tggggccgac gctggtatct ggtcgcgttc gatctcgagc gcgatgattg gcgcagcttc
     4861 cgggtagatc ggatgtcgct gcgaccgccg acacgacgca ccttcacgcc tcgcgatctc
     4921 ccggccgacg acgtcgccgg ctacgtgctg cgcggagtcg cctccgccgg ctggcgcttc
     4981 cgcgcccgcg tgctgatcca cgcacccgcg agcgcggtcg cggacaagat cgatccgtcg
     5041 gtcggcctgg tcgaggcggt cgacgacgcg cgatgcgtcc tcgccaccgg cgccgatcat
     5101 ccgatgacgg tcgccgtgca cctcgccctt ctcgatgagc cgttcacggt cgacgggccg
     5161 ccggagctca gcgacgccat ccgcacgctg gctcgccgct acgccgaggc gacggcgtag
     5221 ccccgcgatc gagcctcact ccgaggtgaa ctgcacctcg ggctcgaccc cgagatacgc
     5281 ggcgcagcgc cgggccgccc tctggatctc cgtacgctcc cgcaaccgga gcccgcggtg
     5341 cggcgtgacc tcgaagacca ccctgcgagc atccacgctg cggcgcattc cggccacgag
     5401 ctggccgtcg cacagcacca tgcctatcga cgcctcgcgc tcgcgcggaa cgatgccttc
     5461 ggagtcgaga gcccagcggg agtcctgata gccccggtac atctcatcga ggatctgtag
     5521 caggtgcgcg ggtccgcgtc cggccgggga ttcatccccg ggggcatgcc agtacgtgcg
     5581 tccgtcgtgg tcgaagcact ccagccgggc ggagacggcg gcgatccccc gccgcgcgtc
     5641 cgtcagcgtg agggtcgacc agtaggcgag atcacgatcc gtcgcaggac cgtgcgcagt
     5701 gagatagcgg aacgcgagcc gagcgagcgc ctcgtcgcgg tcgaccgcgg cagcaggtgg
     5761 catccgctcg tccatgagcg cccaggtgtg ctgaccgtca cgtggtgcgc cactgctgag
     5821 caggcattgg agctcgagcc ggcccagcag cagcgtcagc tgcgacgcgg gcaggtcaag
     5881 cccggcatcc gcgagggtcg ccgcgatctc cgcacgggtg cgtccggtgt cgccggatac
     5941 cgtccgcatg atcgcgtcgg tgagctcggt gatacggtcc gccgaaggcc gcagctgctg
     6001 atcgaaaacg ggcagcaccc gcggtgcggt gagctcgatc acccagcgcg cgtcgtccct
     6061 gtgcacgtag tgccacgtcg gtcgcagcac gtgcgtccgc acgatgcgcc cctccgtcag
     6121 tgcgatcgcc agatcctccc ggtcgggggc tgctgtccgc gccgcgacgg cccaggcgga
     6181 ctgcgcgggg ttctccgcct gcacggcgag cagcgtgcgg acgacggttt cggcatccgc
     6241 ggccggcgct tccagcagct gggagcgcag ccggcgccgc gcgatccccc gcagcgtcac
     6301 cccgtcagct cccccggcag cggccagcgt ccccgggcct ccttgaaccg gatcgccgcg
     6361 agcagcggag acatgtcgta catggcgggg ttcaaggcga gcttggctcc gttcgaagcc
     6421 ctgatccgca ggttcggctg cccgttcgcc tcggtcatcc tgtagtcgac gatgtcgctg
     6481 tagatgatcc gatcctcgcg acccagcagc gtgcggtaat gcaccgcatc ccgctccggc
     6541 gcgacgtacc agttgcggta catcatcagg aacagcaccc cgccgaacag gatcgcgacc
     6601 gaggcgatcc ggaacgcggt gggatcgtcg gcatcgctcg aggtgaacgc caccagcgac
     6661 atcagcacgc cgacgaccag cagcagccac ccgacgacgg cgacgagctt cggcatccgc
     6721 tgccggtccg gatgctgctt cgaacgattc ggatgccgtc gcatccacca caccaccgcg
     6781 gggacgagcg ccaccaccac gatcagcagc gcgaccttgg tgagtgcgac ggtgctcatg
     6841 aaccgacggt atcaacgccg ggacgccgaa cggcggagcc cgatgctccc atcggactcc
     6901 gcctcggatc gacgggtcag tcggcgtaga gcgccaggcg tcgggtcgcc gtcgcactgt
     6961 gcgcggccat cccctccgag tccgagatca cctgcaccgc gttcgcgagc tccggggtcg
     7021 cgggctgcgc gatgcgctgg tacgtgagcg gcttcaagaa gcgcgagacc gacagaccgg
     7081 ccgagtgctt ggcaccacct gcggtgggga gcacatggtt ggtcccggcc atgcccttgt
     7141 ccgagtacgc gacagtgctc cacgggccga tgaacagcga gccgtagttg cgcagccggt
     7201 tgtggtacca ttcgtcgtcc gcggtgatca gctcgagatg ctcgggagcg aggtcgtcca
     7261 tgagggcagc ggcggtctcc ctgtccttcg ccacggtcac cgatccgtag tcgcgccagg
     7321 cggcaccggc gatctcggcg gtcgcgaggg tctccagctg cgcatcgacg gcggcgatga
     7381 ccgcgcggcc gtgctcctct gaggtggtca cgagcgcggc gggcgagttc ggcccgtgct
     7441 cggcctggcc gagcagatcg gccgcgacga tctcagggtc ggcggtctcg tccgagatca
     7501 cggcgacctc ggaagggccg gcgagcaggt cgatcgcgac cgtgccgaac agctggcgct
     7561 tggcctcggc cacgaaggca ttgcctgcac cgacgagcat gtcgacgggc agatcgccga
     7621 gcaggccgta cgccatcgcg gcgagcgcct gcacaccgcc cagcacgaag acgcgatcga
     7681 caccggagag gtgcgcggcg tagacgacgg cgtcgttggc tccgccatcc ggctgcggcg
     7741 gggtgcacgc gatgacagtc ggcaccccag cggccttggc gacccccacg gtcatgaagg
     7801 cgctcgcggt gagcgggaac cggccggcgg gaaggtaggc gccgacacgg gagaccggaa
     7861 tgtacctggc gccggtcacg agcccgggca cgagctcggt ctcgaagtcc gcgagatgct
     7921 cgcgctgctt gcgtgcgaac gcctgagtgc gctccgcgcc gagctcgatc gccgcacgga
     7981 ggtcggcggg cagccggtcg ccgctggtgg cgatctgctg cgcggacagc tcgatgtcgc
     8041 ctccgtcgta acggtcgagc ttgcgcgcgt agtcgagcac ggcatccatg ccgcgcttct
     8101 ggatgtcact gagcatggcc gacacggtct cgatgaccgc ggggtcgcgc tgcgcggcag
     8161 gggcgtcgat gaggggagtc ttgaggtgct ggaaggagcc ggcgaggctc tgaagcacag
     8221 gtgagctgta acgcatgtca gcaggttagg tggccgctag cgatggtaaa agggagcaga
     8281 acctgagcca ggcatagggt ttaccattgg ccatgaccat cgctcagctg agggccttcc
     8341 tgctcgctct ggaactgggt tccttcaccg ccgccgcctc cgaactgggc atgacgcagg
     8401 cctcggtgtc cgagctcatc gcacgcctcg aacgcgagct gggcgtgccg cttttcaccc
     8461 gcggagggcg ccggctcgtg ccgacggcgg cggcggtcga gctgcgcccg cacgccgaac
     8521 aggccatcgg cgcgatccgc aacggtgtcg agacggtgcg ctcgctcacc gccctggagg
     8581 gcggcacctg caccttcggg gttctgcgca acgccgcgta ctacgcgctg gccgatctcg
     8641 ccgagacctt ccattaccgc catccgaacg tgcgcgtccg gctcaccggc atcaactctg
     8701 ccctggtggc gcgctcgatc gcccgcggcg aggtggaggc gggcctggtc gtgctcccgg
     8761 tggaggagaa cgggctcagc atccgcccac tgttccgcga cgaggtgctc tacgcctcgg
     8821 cgcagcgtcc ggcgtccgcc ggcgccgtga cgatcgacga gacagccgac gcgaaactgg
     8881 tgctctacga cgcgttcgcc ggctgggatg accccacccg gcgccagctg ctggagcgcg
     8941 cacgactgcg cggcctgcag ctcgaaccgg cgatcgaggt ggagcacgtc gagacggcgc
     9001 tgagcctggt cgcatcgggg gcggccgaca cgatcgtgtc acgcacgatc gccgagtcgg
     9061 acgccttccc cgagggcatc cgcaccttcc ccttcgccga accgatgtac gacacgatcg
     9121 cgctcgtgca gcgcgaaggc gtggtgctgt cgccggcgac gcagcggctc gcggagctgg
     9181 cggagcggat gctgctggag agcgtgtccg agcacggcgg gcggacgcgg ctggtctgac
     9241 ggagaggtca gtagagctcg ggcctgcggt ccgtgaagag gtggttgtac gggggcaggg
     9301 atttgtcgcg gagggagtcg aggtcgagct cggccatcgc gaagcgctcg tcttcggcgg
     9361 ccagccagcc cagcccgtcg accacggcgg tgccgccggt ccattccacg ccgcgctcgg
     9421 aaccgtggtg gtcggcgatg acgatcggca tccgcgagga gcgcgccgac cccatcgcct
     9481 ggatcgtctc gggcggatgc tcccccgcgg gacgcggcac agccggccag gccacgggca
     9541 gagcgagcac atcggccccg gcaagggcga tccggcgcgg cacctccggg aactcgtcgt
     9601 cgtagcagac cgccaggccg aggcgcccga acggcgcatc caccacggcc ccggcctcat
     9661 gaccgggggt gaacagattc acctcagctc cccagaggtg cgccttgcgg tagtcgtgca
     9721 gccgccgatc gcgcgtcagc gctgccgagg tgttgtacgg cccgccgtca tcgtcctcgg
     9781 cgtatccgac gacgacgacg gcgctctcgg acagggcatc ctgcagcagc ctccagcgcg
     9841 gatcgtcccg gcgcagcgca gcggccagca cctcgtcgcg atcggtgaac atgcatccgc
     9901 tcgtcgcgag ttcgggcagc acgatcagac cggcgccgcg ccggacggca tcccggatca
     9961 cgtcagccag ccgggccagg ttcccgtcga cgtcgccgat gcgcacctcg ggcgagatcg
    10021 ccgccaccgt cagcatcgtc atgtcagcat cctctcgtga tcgggccgcg ccccgtgccg
    10081 cacagcaggt gtgagcggga ccgggatcac ccggccccgc tcaccgtcgt cctgtcagtc
    10141 gtcctcggtg gccgtgacca tcacgccgag gggcgcgagg tagccgttgg ggtcgatggt
    10201 ggcgtccgcc gcggcggccg cctcgacgac ctcgggtgcc cactccgggt acacgcggtc
    10261 gtcgccgccg ttgatcacga gcacctcgcc cgcgcccgac tcgccgttct cgacgcaggt
    10321 gaaggatcgc caagcgccga cgggcaccga gaacgagtcg ccgggaccga agcgcagcgc
    10381 cgtcgccggg tcgccgttga gcacgacctc ccattgaccc gacttgatca gcagggcctg
    10441 ggtctgctgc tggcggtagc gcagcacgcc ctgtccggcc tcggcgcgca cccacgcgag
    10501 gttgaacgag tgcggctcgt ggatcgacgg cacctgccgg cggttctgcg tcatgccgta
    10561 gccgatcacc agcgcgaggt cgaccctgcc accgtcgacc gcggtgcaca gcagcgcatg
    10621 cgagccgtag cgacggtcgc ccggctgcac gacgcgggag cgcatctcct cgacggagta
    10681 cgaggtgagc tcgtcgatgt agtgctgctt catcggcgtg atcagcggca ccccctccgg
    10741 cagctcgtca ccggccacgg tgtcgatcag ccggttgtcg gcggtgaggt gcaggccgta
    10801 ggactcggcc tcccgcagca ccgacggccc ccagatgatg ccgcccgtgt cgtcgcgccc
    10861 cagcacggtg tacagcacgc cgtcgtccgg gccctcgttc gtgaagccgc ggaagatcca
    10921 ggtcgggatg gagatgatgt cgccgtcatg gctgatgtac tcgccctgct tgccgtcgac
    10981 gccccaccgc agccggaact cgccgccgag gttgacgaac acctcggcgg tgaagtgcag
    11041 gtgcaggttg ttcgagatgc cgttcggcat gccggcggcg ccggtgttga agccgtgctt
    11101 ctcggtcagg ttgatgtact ggctcgcgtt ctgcgagacg cccacaccga tgaaggagta
    11161 gttctccttc ttgtcggagc cgggggttcg gcagtcgatg aaggccgcgt tgcaggcgac
    11221 ccagtcggac ttgcggatcg tccgtcgacc gatctcggcc gcgtcgacga tgacgtcgtt
    11281 catttcactc tctccttgtc gcccgggatg cgggctgttg ttcgttgtct cagtagatgt
    11341 tcgaggggcc gaccggctcg tcgccgcgca tgcccgcgat ctgcgcgcgc agggcgatgt
    11401 cgtcccacag ccgcacctcc ttgaccaggg ctccctggtg gaaggtgaac tgcgagatgc
    11461 cgagcacgtc gacgggcttg ccggtgagcg ggccgtagtt cgcacggccg ttgtagtcgc
    11521 cggtgaactt ccaggtgacc gccacgcgca ggccggcgta gcgcacgtcg tagttggtct
    11581 ggatgtcgcg gatctcgaac tgtccggccg ggaacgactc caggaagcgc agaagcgcac
    11641 ggcggtatcc ctcagggcgg atgatcgtgc ggtcgccgac ggtgagcagc accagatcgc
    11701 gctggaagta gcgctcgacc ttctgcaggt cgcggtcgtt ccacaccgtg ttgatcatgt
    11761 cgatcaccag ttcgacctcg ctgcggtagt cgtccgggcg ggcgccggaa tccccgaccg
    11821 cgatcgggtc ggcgggagcc gcttcggtcc acgagcccag gtatccgcgg aacgcctgcg
    11881 cccgagcgag ctcgtccagg tcggcgccgc tccgcagtgc gccggcgagc gagtcgcgca
    11941 ccacccattc ctcgaccatg cgaccccgcc ggtacaggca gttcgcgatg gtgcggctga
    12001 caccgacctc gttcaggtgc ccggagagca ccaggtgcga gctcaggaac gcgtcatcgc
    12061 cgcgcgcctc ccagatgacg tcctcggcct gaccggtgcg gtcgggagtg tccgagatgc
    12121 gcatcagggt gccctcgatg acctcggcgc ggctcgtgga ggtgccgtag gcgccgtgca
    12181 cgatcgagtc cggctcgtag ttctcccgga tgtagctgat cgaccgatcc acccagatga
    12241 ggtcggtcac ctcgcggatg aagtcgtcgg ggtccttgta cggctggtat gcgaccgggt
    12301 cgagaggcac gctgttgctc ctttgctgct gtccttgtcg gcgaacctcg gagagatcgc
    12361 cgatgttggc agtgtatgcg tatgcacaaa gggatgcaat agcggatccg agaacacgcg
    12421 cttgtcagag cgcgcgaccg tcggctcgct gacgccgcgg gtcagcgcgg gcgaggagcg
    12481 gcgacggtgc tgcgcggcac gaacgaggag gcgaaggtga tctcgctccg ctcggcgtcc
    12541 ggatgctcga tgcgacggac gatgagatcc gccgccgcgc gggccatgcc ggtgatgtcg
    12601 tatccgatcg tggtgaggtc gacgatcggc caggatgcct cgggcaggtc gtcgaagccg
    12661 atgatcgaca catcgcccgg caccgagagc cccgcccgac gcgccgcgtt cagcgcaccg
    12721 taggcgacca cgtcattgcc gcagaagagc acggtgggcg gctcgtcccc tgcgagcagc
    12781 tccgcggcag cggcctcgcc ctcgaccgcc ccgtac
//
LOCUS       DATMSX010000266         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_164907,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000266 DATMSX010000000
VERSION     DATMSX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164907"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3221
                     /locus_tag="VN041_08080"
     CDS             <1..>3221
                     /locus_tag="VN041_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HWU29034.1"
                     /translation="TTGVFTLTDTAGAGLDFGAVTSAGAFTCNAADPLICTLPAGTAV
                     GTYSLTYTAVVNASATGTVTNAVVGTGGGEGNPACSGTCDTSTTVTSPAVTYAKSANV
                     TEARVGDTIEYSLTATVTNSPTTDIVTLTDTPGAGLDFTAVINAGAFACNAEIPLVCT
                     LPAGTAVGTYSLTYAAVVNATASNGSVTNAVVGTGGGGGNPTCSGVCDTNTPLIAPEV
                     IFAKTASTAGPVKAGDVITYTLTTTIANSKTTSDVVLLTDTLGTGIDFTAVTSAGAYG
                     VDASGAPVVRFTLPAGTGPGTYAVSYTATVNDQASGSVSNVVVGSGPDNPVCTTACGT
                     DTSVKDPVVTYHKSVSAPGATVKVGDTLSYTLTATVADAATTDIVTLTDTPGAGLDFT
                     AVTIAGAFTCNAASPLVCTLPAGTAVGSYSLTYTATVNATASGTVTNAVVGSGGGGGN
                     PACSGACDTSTIVTPSTITYGKTTSATNAKVGDILTFTLTATVADAATDAIFTLTDTP
                     GAGLDFTAVTSAGAFTCNAASPLVCTLPARTAVGTYSLTYTATVNATASGSVTNAVVG
                     TGGGGGNPACAGACDTDTPLVAPEVIFAKTASTAGPVKAGDVITYTLTTTIANSKTTS
                     DVVLLTDTLGTGIDFTAVTSAGAYGVDASGAPVVRFTLPAGTGPGTYAVSYTATVNER
                     ASGTVSNAVVGSGTDNPVCTKACGTETNVIAPAVTYAKSANVAQAKVGDTIEYSLIAT
                     VTNSPTTDVVTLTDTPGMGLDFTAVTSAGAFTCNAASPLICTLPAGTPTGTYSLTYSA
                     VVNASASNGSVTNAVVGTGGGGGNPTCSGACGTNTPLVAPEVVFAKSASTAGPVKAGD
                     VITYTLTTTIANSKTTSDVVLLTDTLGTGIDFTAVTSAGLYTVDASGAPVVRFTLPAG
                     TGPGTYAVSYTATVNDRASETVSNVVVGSGTDNPTCTTGCGTETNVIVPTVTYAKNVD
                     ATQAKVGDTLTYTLTATVADAPTIEIVALTDTLGSGLDFGAVTSAGAFTCIGAGPLVC
                     TLPAGTAVGTYSLTYTATVNATATGSVTNAVVGTGGGGGNPACA"
BASE COUNT          572 a         1137 c         1041 g          471 t
ORIGIN      
        1 accacgggcg ttttcacgct caccgacacg gccggcgccg gcctcgactt cggcgcggtg
       61 accagcgcag gcgccttcac ctgcaatgcg gcggacccgc tcatctgcac cttgcccgcg
      121 ggtactgcgg tcggcaccta cagcctcact tacaccgcgg tcgtcaacgc cagcgcgacg
      181 ggtaccgtca ccaatgccgt tgtcggaacg ggcggcggcg aaggtaatcc ggcctgctcc
      241 ggaacctgcg ataccagcac gacggtcacg tctccggcag taacttatgc caaaagcgcg
      301 aatgtgacgg aggcaagggt cggcgacacg atcgaatata gcttgaccgc gacggtcacg
      361 aattcgccga ccaccgacat cgtgacgctg accgatacgc cgggggcggg tctcgacttc
      421 acggcggtga ttaacgcggg ggcgttcgcc tgcaatgcgg agattccgct ggtctgcacc
      481 ttgcctgccg gcacggcggt cggcacctac agcctcacct acgcagcggt cgtcaatgcg
      541 acggccagca atggcagcgt caccaacgct gtcgtcggca cgggtggcgg cggcggaaat
      601 ccgacatgct cgggtgtctg cgacaccaac acgccactga tcgcgcccga agtcatcttc
      661 gccaagaccg cgagcacggc gggaccggtg aaggcgggcg atgtcatcac ctatactctg
      721 accacgacga tcgcgaattc caagacgacc tcggacgtcg tcctgctcac cgacacgctc
      781 ggcacgggca tcgacttcac cgcggtcacc agcgcgggcg cctatggtgt cgacgcctcg
      841 ggtgcgccgg tggtgcgctt caccctgccg gcgggcacgg gtcccggcac ctatgccgtg
      901 agctacaccg cgacagtgaa cgaccaggcg agtggatcgg tcagcaacgt cgtcgtcgga
      961 tcggggcccg acaaccccgt ctgcaccacg gcctgcggca ccgacacgag cgtcaaggat
     1021 ccggtcgtca cttatcacaa gagcgtctcg gctccgggcg cgacggtcaa ggtcggcgac
     1081 acactgagct atacgctgac cgcaacggtc gccgatgccg ccacgacaga cattgtcacc
     1141 ttgaccgaca cgccgggcgc gggtctcgat ttcacggctg tgaccatcgc gggcgccttc
     1201 acgtgcaacg cggcgagccc gctggtctgc accttgcccg ccggcaccgc ggtcggcagc
     1261 tacagtctga cctacaccgc gacggtcaac gccaccgcga gcggcaccgt caccaacgct
     1321 gtcgtcggct cgggcggcgg cggaggtaat ccggcctgct cgggcgcatg cgataccagc
     1381 acgatcgtca cgccctcgac gattacctat ggcaagacga cgagcgcgac gaacgcaaag
     1441 gttggcgata ttctgacctt tacgctcacc gcgacggtcg cggatgcggc gacggacgca
     1501 atcttcacgc tcaccgatac gccgggtgcg ggtctcgatt tcacggcggt gaccagcgcg
     1561 ggcgccttca cgtgcaacgc cgccagcccg ctggtctgca ccttgccggc ccgcaccgcg
     1621 gtcggcacct acagcctgac ctacaccgcg acggtcaatg ccaccgcgag cggcagcgtc
     1681 accaatgccg tcgtcggcac cggcgggggc gggggcaatc cggcctgcgc gggcgcctgc
     1741 gacaccgaca cgccgctcgt cgcgcccgag gtcatcttcg ccaagacggc gagcaccgcg
     1801 ggtccggtga aggcgggtga cgtcatcacc tacacgctga cgacgacgat cgcgaattcc
     1861 aagaccacct cggacgtcgt cctgctcacc gatacgctcg gcacgggcat cgatttcacc
     1921 gcggtgacca gcgcgggtgc ctatggcgtg gatgcttcgg gtgcgccggt ggtgcgcttc
     1981 accctgccgg cgggcacggg ccccggcacc tatgcggtga gctacaccgc gacggtgaac
     2041 gagcgggcga gcgggaccgt cagcaacgcc gtggtcggat cgggcaccga caacccggtc
     2101 tgcaccaagg cctgcggtac ggagacgaat gttatcgccc cggccgtgac atacgcgaag
     2161 agtgcgaacg tggcgcaggc gaaggtcggc gacacgatcg aatatagctt gatcgcaacg
     2221 gtcacgaatt ccccgaccac ggacgtggtg acgctgaccg acacgccggg catgggtctc
     2281 gatttcacag cggtaaccag cgcgggcgcc ttcacatgca acgcggccag cccgctgatc
     2341 tgcactctgc cggccggcac gccgacgggc acttacagcc tcacatattc ggcggtcgtc
     2401 aacgcgtcgg ccagcaatgg cagcgtcacc aacgccgtcg tcggcacggg tgggggcggg
     2461 ggcaacccga cctgctcggg cgcctgcggc accaacacgc cgctcgtcgc gcccgaggtc
     2521 gtcttcgcca agtccgcgag caccgcgggg ccggtgaagg cgggcgacgt catcacctat
     2581 acgctgacca ccacgatcgc gaactcgaaa accacctcgg acgtcgttct gctcaccgac
     2641 acgctcggca cgggcatcga tttcaccgcg gtgaccagcg cgggcctcta cacggtcgac
     2701 gcctcgggtg cgccggtggt gcgcttcacg ctgccggcgg gcacgggccc cggtacctat
     2761 gccgtgagtt acaccgcgac ggtgaatgac cgggcgagcg agacggtcag caacgtcgtc
     2821 gtcggttcgg gcaccgacaa tccgacctgc accaccggct gtggaaccga gacgaacgtc
     2881 atcgtcccga ccgtcaccta cgccaagaat gtggatgcga cgcaagcgaa ggtcggcgac
     2941 acgctgacct ataccctgac cgctacggtc gccgacgcgc cgactatcga aatcgtcgcg
     3001 ctgaccgaca cgctgggctc cggcctcgac ttcggcgcgg tgaccagcgc gggagcgttc
     3061 acctgtattg gagcgggccc gctggtgtgc accttgccgg ctggcaccgc ggtcggcacg
     3121 tacagcctga cctacaccgc gacggtcaat gccaccgcca cgggcagcgt caccaacgcc
     3181 gtcgtcggca cgggcggcgg cgggggcaac ccggcctgcg c
//
LOCUS       DATMSX010000267         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_164978,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000267 DATMSX010000000
VERSION     DATMSX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164978"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VN041_08085"
     CDS             <1..1155
                     /locus_tag="VN041_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU29035.1"
                     /translation="IAAVIRPLGWTVMALAVAAWITGQLLGWQEVVVTGLVLAAVAVI
                     CALFLIGRTEYDVSLDLARTRVVVGERAVGALTLLNRGSRAILPSRVVLPVGSGRGEF
                     SIGRLAPGQEAEELFAIPTQRRGVVKVGPVSVVRGDPLGLFERAHRRDDPVDLYVHPK
                     TTLFGGQSLGFLRDLEGLPAADLSRDDVSFHALLEYQPGDDLRHVHWRSTARTGTMMV
                     RQFEETRRSHFVIGLTRSPRDYRSDEDFELAISVAGSIGLRALRDSQKVDVRVQGREL
                     PAGTGKQLLDSLSMVEHSRPRDGGVPELAGVLAATTASASVVALVCGSAVSSEDLRLA
                     CSRMPFGARVIAVVVQGGVEQPALRRIADADVVTIGALDQLPLALQKVLA"
     gene            1152..>1854
                     /locus_tag="VN041_08090"
     CDS             1152..>1854
                     /locus_tag="VN041_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWU29036.1"
                     /translation="MTSASMTSSTSKGAPLPLRRWILDLAATAVLMVVALLGFWPSFA
                     GPSFLGAALGGMLLGLAIAAVCTWRRWGILIIAGLTIAAYFLFGGALALPHTALAGFV
                     PTIETLKSLALGAVTSWKQLLTTVAPVSAADGHLVVPFIVALVATVTTASLALRLRQV
                     WWALIPAASALILVIALGVPDPAFPLVQGVIFAAVTAVWMSLRVWWAPQNSAVDVSEV
                     DPTRAAHMRTRRLLGG"
BASE COUNT          197 a          686 c          655 g          316 t
ORIGIN      
        1 atcgcggccg tcatccgccc cctcggctgg acggtgatgg cgctcgcggt cgcagcatgg
       61 atcaccggtc agctgctcgg ctggcaggag gtcgtggtca ccggcctcgt gctggccgcg
      121 gtcgccgtca tctgcgcgct gttcctgatc ggccgcaccg agtacgacgt ctccctcgac
      181 ctcgcgcgca cccgtgtggt ggtgggggag cgcgcggtgg gcgcgctgac cctgctcaac
      241 cgcggatcgc gcgcgatcct gccctcgcgg gtggtgctgc cggtcggctc cggccgcggc
      301 gagttctcga tcggccgtct ggctcccggt caggaggccg aggagctgtt cgcgatcccc
      361 acgcagcgcc gtggcgtcgt caaggtcggc ccggtgagcg tggtgcgcgg cgacccactg
      421 gggctgttcg agcgcgctca ccgtcgcgac gacccggtgg acctgtacgt gcacccgaag
      481 accacgctgt tcggcggcca gtccctcggc ttcctgcgtg acctcgaggg cctgccggca
      541 gcggacctgt cgcgcgacga cgtctccttc cacgcactgc tggagtacca gcccggcgat
      601 gacctgcgcc acgtgcactg gcggtcgacg gcccgcaccg gcacgatgat ggtgcgccag
      661 ttcgaggaga cccgccgctc gcacttcgtg atcggtctga cgcggtcgcc ccgcgattac
      721 cgcagcgacg aggacttcga gctcgccatc tcggtcgccg gatccatcgg cctgcgcgcc
      781 ctgcgcgact cgcagaaggt cgacgtgcgc gtgcagggtc gcgagctgcc tgccggcacc
      841 ggcaagcagc tgctcgactc gctgtccatg gtggagcact cgcgtccccg cgacggcggc
      901 gtgcccgagc tcgccggcgt gctggcggcg accacggcat ccgccagcgt cgtcgccctg
      961 gtgtgcggct ccgccgtctc cagcgaggac ctgcggctcg cctgctcgcg gatgccgttc
     1021 ggcgcgcgcg tgatcgccgt ggtcgtgcag ggcggcgtcg agcagcccgc gctgcgccgc
     1081 atcgccgacg ccgatgtcgt cacgatcgga gcgctcgacc agctccccct cgctctccag
     1141 aaggtgctcg catgacctcc gcatcgatga cgtcatcgac ctcgaagggt gccccgctgc
     1201 cgctgcggcg ctggatcctc gacctcgccg cgactgccgt gctcatggtg gtggcgctgc
     1261 tcggcttctg gccgagcttc gccgggccgt ccttcctcgg tgcggctctc ggcgggatgc
     1321 tgctggggct ggccatcgcg gcggtgtgca cctggcgccg ctggggcatc ctgatcatcg
     1381 ccgggctcac aatcgcggcc tacttcctct tcggcggtgc gctcgcactg ccgcacacgg
     1441 ccctggccgg gttcgtgccg acgatcgaga cgctgaagag tctcgccctc ggcgcggtga
     1501 cctcgtggaa gcagctgctc acgacggtgg ccccggtgtc cgcggcggac ggccacctcg
     1561 tcgtcccgtt catcgtggcc ctggtggcga cggtgacgac cgcctcgctg gccctccggc
     1621 tgcgccaggt gtggtgggcg ctgatccccg cggcatccgc tctcatcctg gtcatcgcac
     1681 tcggcgtgcc cgaccccgcc ttccccctgg tgcagggcgt gatcttcgcc gcggtgacgg
     1741 ccgtgtggat gtcgctgcgg gtctggtggg cgccgcagaa cagtgccgtc gacgtctccg
     1801 aggtcgaccc gacccgggct gcgcacatgc gcacgcgccg gctgctcggc ggtg
//
LOCUS       DATMSX010000268        10107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13513,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000268 DATMSX010000000
VERSION     DATMSX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13513"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VN041_08095"
     CDS             complement(<1..398)
                     /locus_tag="VN041_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWU29037.1"
                     /translation="MIITVTPNPALDLTWRVAALEIGGVHRADAGKSRAGGKGLNVAR
                     VAHAQGASAVAITTSGGATGAEFAAELDASGVPHMLVPVTASTRRSIALVDEALGDTT
                     IVNERGEAPTDAEWAALKDAVARALARASV"
     gene            complement(395..523)
                     /locus_tag="VN041_08100"
     CDS             complement(395..523)
                     /locus_tag="VN041_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29038.1"
                     /translation="MTSALSGTDARSGNTCTIGSPDASCIRSLIVQVSSHEATVAA"
     gene            complement(520..1506)
                     /locus_tag="VN041_08105"
     CDS             complement(520..1506)
                     /locus_tag="VN041_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU29039.1"
                     /translation="MTEHDRIPDVVKDESGERLAAAAGIGAGAPVLAFDVGGTDIKSA
                     LFDATGAAVGLRRTPTPVGGDDPVGALIDRLAELAEELREQHPEIVPEAIGLVVPGIV
                     DADSGIAVFSSNLGWRDAPIRDLTAARFGLPVAFDHDVRSASWAERVLGGARDYADAV
                     VLIIGTGIAGALLVGGQPYTAGGYAGEIGHSPIGEWPCPCGARGCLEARASAGAISKR
                     YAEETGTVVDGAREVIALASAGDETGARIWNEALDALAMSIAQLTAVMAPQAVVIGGG
                     LSRAGGALFDELRTRLADRLSFHRIPVLVPAELSGNAGILGSALRAREAAHA"
     gene            complement(1515..2414)
                     /locus_tag="VN041_08110"
     CDS             complement(1515..2414)
                     /locus_tag="VN041_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWU29040.1"
                     /translation="MTNAAGAHMRAELTSQPETWARAAGMRAEQALLPASGARIAVVG
                     CGTSWFMAQSYAALREAAGHGETDAFTASEFPYGRGYDAIVALTRSGTTTEVLELTER
                     VKGKTPVVAVLGDDTSPLVDLADTVIALPFADEQSVVQTRFATTALALFRASLGEDLD
                     AAIADAQAVLDGQASDDPALTDAEQYSFLGMGWTFGLAHEAGLKMRESSQSWTESYSS
                     MEYRHGPIAIAAPGRITWQFGAAPDGLQAQVEATGARFVQHPIDPMAELVRVHRVALD
                     RAVGKGLDPDQPRNLTRSVILDD"
     gene            complement(2613..2909)
                     /locus_tag="VN041_08115"
     CDS             complement(2613..2909)
                     /locus_tag="VN041_08115"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWU29041.1"
                     /translation="MSDEAARAELLRLRASIDNIDAALIFLLAERFRATQQVGHLKAD
                     HEMPASDPKREEQQVARLRALAEEAHLDPEFAEKWFNFVVAEVIRHHTEAAEGR"
     gene            3055..4233
                     /locus_tag="VN041_08120"
     CDS             3055..4233
                     /locus_tag="VN041_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWU29042.1"
                     /translation="MSADESVKSEPTAESATVQAVVVEDAAPSPAPPVVEPPLPSRSF
                     WTRIDKPFAFGFLVALGGLAAILLALTLSNLSTVLIYIAFAMFAALGLDPAVRFLERR
                     GLSRGVSVLAVILGLVVVLALILWMIIPVVVEQVTLFVKSVPHLITDFMHSDVYAFLE
                     KQFGDQFQQLVEDAQKFLTDGKTWQAVGGGALQVGSSIATGISGFIVVLVLTLYFVAG
                     LPTIKTALLRFAPARDRERARGITDQITDSVGGYVQGMVILAFANAIVALVLYLALGL
                     PFPPLMATVAFLVTLIPLVGSVLFWAIGSVIALFADPWHALIFAAVYLVYMQVEAYFL
                     TPRVMNKAVAVPGALVVIGALAGGTLLGLLGALVAVPVTASILIIINQVWLPRQDARV
                     "
     gene            complement(4258..5178)
                     /locus_tag="VN041_08125"
     CDS             complement(4258..5178)
                     /locus_tag="VN041_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU29043.1"
                     /translation="MITARKSRLALAVGVALAATLALAGCGSSNPLDEPSTGGGTDKG
                     GETIVVGSQAYYSNEIIAEIYAQALENAGFTVEKKFNVGQRDAYMPDVESGKINLFPE
                     YTGNLLEYLDKSATATSPDDVYAALQKALPKTLKALDYSKASDQDTYTVTKKFAQDNG
                     LESIADLAKLNTKPVLGGAPELEQRPYGPAGAKKIYDVDLGFSATAQSTLESLLAGQV
                     QVADIYTADPAFQTKDIVALKDPKNMILASNVVPIASSDIADKIAKVINPISAALGAE
                     DLVSMNVQSTVDQASAEDIAAKWLKAKGLD"
     gene            complement(5211..5954)
                     /locus_tag="VN041_08130"
     CDS             complement(5211..5954)
                     /locus_tag="VN041_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29044.1"
                     /translation="MNLFTDAIAWLLNPAQWQGQYALPTLLGQHLLYTAISVLVAAAI
                     ALPAGWLIGHTGKGREIAVAVSGAARAVPSFGLLILLVLLLGVLQTPLAAVITFVLLA
                     IPSLLAGAYTGFEAIDRRVIDAARAVGMTEWQIFTKVELPLGMPLLVGGIRSALLQVI
                     ATVTIGAYVNLGGLGWPIIQGIPLRRFDQVLAGALLVAVLALLVDLLLAALQRAAVPT
                     GIRSPSPDRTSSSDRNGRRSARRAASVTA"
     gene            complement(5951..6649)
                     /locus_tag="VN041_08135"
     CDS             complement(5951..6649)
                     /locus_tag="VN041_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU29045.1"
                     /translation="MNWVGDNLDLILSLTLVHLKQSATPIVLGLLLSVPLGWVAWRYR
                     LVRGPVIVLTGLLYTIPSLALLILLPALFGYSATDEPNLIIALTIYAVAILVRAVADG
                     LDSVDPDVRQAATAMGYAAPRRFFTVELPLAGPVILAGLRVAAVSTISLATVGILIGV
                     ENLGYLFTNGLQRRIIAEVLAGVVAVVVIALVVDVLLVLLGRMLMPWTRATAAGGGAL
                     SPSKARRAVVRSAA"
     gene            complement(6651..7469)
                     /locus_tag="VN041_08140"
     CDS             complement(6651..7469)
                     /locus_tag="VN041_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU29046.1"
                     /translation="MIEFTSVSKRFPDGTLAVEDFSLVLPSRKTTVFVGSSGCGKTTL
                     LRMINRMVDPTSGTIAIDGESVLDRDPVRLRRSIGYVMQNSGLMPHFTVIDNVSTVLR
                     LNGVSRTEAHGRSRELLSTVGLDASLADRYPSQLSGGQQQRVGVARGLAADPNILLMD
                     EPFGAVDPIVRADLQQELIRLQVELDKTVVFVTHDIDEAFLLGDQVVILDKGARVVQV
                     GSPSEIIENPADDFVEAFIGAERGRRALSLKQTPRGTVVVDSEGRTQGVLKDGT"
     gene            complement(7662..9059)
                     /locus_tag="VN041_08145"
     CDS             complement(7662..9059)
                     /locus_tag="VN041_08145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU29047.1"
                     /translation="MSDEYDLIVLGGGPVGENVADRAVQGGLTAVIVESELVGGECSY
                     WACMPSKALLRPIQALRAAQHVKGVSGGTLSPADVLARRDSFTSHWKDDGQVDWLKGA
                     GIDLVRGHGRLTGEREVTVGGRVLRARQAVAISTGTDATIPGIPGLADAKPWTSREAT
                     SVDHVPESLAIIGGGVVAAEMATAYAGLGASVTLIARSGLLGGLEPFAGERVAEGLRG
                     MGVDVRLNTATSAVRRDGDHVVVTVGDGEEITASEVLVATGRTPRSGDIGLETVGLEP
                     GRYIEVDDTLRVPGSDWLYAVGDVNGRVLLTHQGKYQARAAGDVIAARSRGEVVADQP
                     WGRHVATADHAAVPQVVFSEPEVASVGLTADAARAAGHEIEVIDYDLGWVAGSGLHAD
                     DYQGQARMIVDTEREVLLGVTFVGADVAELLQAATIAVTGEVPISRLWHAVPAYPTMN
                     EVWLRLLEEYGRQSA"
     gene            9204..>10107
                     /locus_tag="VN041_08150"
     CDS             9204..>10107
                     /locus_tag="VN041_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29048.1"
                     /translation="MAIRGAPVVDWTGRFDQLVAREALLDAEELDDLGQAAWFTGHDD
                     VSHRAWERAHLRHLDGGDLTAAVRCAFWLGFTLSEQGEDVRARTWMGRLVELCVRPEA
                     DAHAAAYRELCGAVAHYAQGDLLGSVPLYRRAAAQAADEGDGDLEALATMGLGRALVR
                     LGEVEEGFGCLDRVMLMIGSDRVSDRAAGPAFCAVVASLLARGDLERARVWTRDLGDW
                     CDTQQGLEPFRGECTLHRATVMQLGGEWGEAAKAADQVCRTEKRIETLGNAWYRAAEL
                     HRVAGRATQARDAYRRATGLGREAQ"
BASE COUNT         1457 a         3479 c         3602 g         1569 t
ORIGIN      
        1 cagaccgacg cccgcgcgag cgcgcgcgcc acagcatcct tcagcgcggc ccactcggca
       61 tccgtcgggg cctcgccgcg ctcgttcacg atcgtcgtat cgcccagcgc ctcatcgacc
      121 agcgcgatgc tgcgacgtgt ggaagcggtc acgggcacca gcatgtgcgg cacccccgag
      181 gcatccagct ccgcggcgaa ctcggcgccc gtcgccccgc ccgaggtcgt gatcgccacg
      241 gcggatgcgc cctgcgcgtg cgcgaccctg gcgacgttca ggcccttgcc tccggcgcgc
      301 gacttcccgg catccgctcg gtgcaccccg ccgatctcca gcgcggcgac gcgccaggtg
      361 aggtcgaggg cggggttcgg cgtgacggtg atgatcatgc ggccaccgtc gcctcgtgcg
      421 aggacacctg cacgatcagg gaacggatgc acgacgcatc cggcgatccg atcgtgcagg
      481 tgttcccgga tcgtgcatcc gtgcccgaca gcgcggaggt catgcgtgcg ccgcctcgcg
      541 agcgcgcagc gcggaaccca ggatgccggc attgccggag agctcggcgg ggacgaggac
      601 cgggatgcgg tggaagctca ggcggtcggc gagccgcgtc cgcaactcgt cgaacagcgc
      661 cccgcccgcg cgcgacaggc cgccgccgat gaccacggcc tgcggcgcca tgacggcggt
      721 cagctgcgcg atcgacatgg cgagcgcgtc gagggcctcg ttccagatcc gcgcgccggt
      781 ctcgtcgccg gcggacgcca gggcgatcac ctcgcgtgcg ccgtcgacga ccgtgcccgt
      841 ctcctccgca tagcgcttgg agatcgcgcc ggcggatgct ctggcctcca ggcatccgcg
      901 ggctccgcag gggcagggcc attcgccgat cggcgaatgg ccgatctcgc cggcgtagcc
      961 gcccgcggtg tacggctgtc cgccgaccag cagcgcgccg gcgatcccgg tgccgatgat
     1021 cagcacgacc gcgtccgcgt agtcccgagc accgccgagc acgcgctccg cccagctcgc
     1081 cgagcgcacg tcgtgatcga acgcgacggg cagcccgaac cgcgcggccg tcaggtcccg
     1141 gatcggggca tcccgccagc cgagattgct gctgaacacg gcgatgccgc tgtcggcgtc
     1201 gacgatgccc ggcaccacca ggccgatcgc ctcgggcacg atctccggat gctgctcgcg
     1261 cagctcctcg gccagttcgg cgagccggtc gatcagcgcg cccaccgggt cgtcaccgcc
     1321 gacgggagtg ggcgttcgcc gcagtccgac ggccgcgccg gtggcgtcga acagcgccga
     1381 cttgatgtcg gtgccgccca cgtcgaaggc cagcacgggt gcacccgcac cgattcccgc
     1441 cgccgcggcg agccgctcgc ccgactcgtc cttgacgacg tcggggatgc ggtcgtgctc
     1501 ggtcatggcg cgtctcagtc gtcgaggatc acggagcggg tcaggttgcg cggctggtcg
     1561 gggtcgagcc ccttccccac ggcgcggtcg agtgcgacgc gatgcacccg caccagttcg
     1621 gccatcgggt cgatcggatg ctgcacgaag cgcgctccgg tggcctcgac ctgcgcctgc
     1681 agaccgtcgg gggcggcacc gaactgccag gtgatgcggc cgggggcggc gatggcgatc
     1741 gggccgtgac ggtactccat cgaactgtac gactcggtcc acgactgcga ggactcgcgc
     1801 atcttcaggc ccgcctcgtg tgcgagtccg aaggtccatc ccatgccgag gaagctgtac
     1861 tgctcggcgt ccgtgagcgc ggggtcgtcg gatgcctgtc cgtcgagcac ggcctgggcg
     1921 tcggcgatcg ccgcgtccag gtcttcgccg agggatgccc ggaacagggc gagcgcggtg
     1981 gtggcgaaac gggtctgcac caccgactgc tcgtcggcga acgggagcgc gatgacggtg
     2041 tcggccaggt cgaccagcgg agaggtgtcg tcgccgagca cggccacgac gggggtcttg
     2101 cccttgacgc gttcggtcag ttcgaggacc tcggtggtgg tgccggagcg ggtgagcgcg
     2161 acgatcgcgt cgtagccgcg gccgtacggg aactcggatg ccgtgaacgc gtcggtctca
     2221 ccgtggccgg cggcctcgcg cagagcggcg taggactgcg ccatgaacca ggaggtgccg
     2281 cagccgacga ccgcgatgcg tgcgccggag gccgggagca gcgcctgctc tgctcgcatc
     2341 ccggccgcgc gggcccaggt ctccggctgc gaggtgagct ccgcgcgcat gtgcgcgccg
     2401 gcggcgttgg tcatgtccga ctcccgagga tgcaagtttg tgagcgatat acgcatgtaa
     2461 cgctcacatt atggcatctg tcggtgcgga ttgtcgagac tccccctgca caaccccgca
     2521 gaaacgcacg actccgcagc cagaacccta tccggatgcg gagttgtgca gaactgcgga
     2581 gatgtgcagc atcccgagag gggaacgcgg gctcagcgcc cctcagcggc ctcggtgtgg
     2641 tggcggatga cctcggcgac gacgaagttg aaccacttct cggcgaactc gggatcgaga
     2701 tgcgcctcct cggcgagcgc acgcaggcgc gcgacctgct gctcctcgcg cttcggatcg
     2761 gacgcgggca tctcgtggtc ggccttcagg tgaccgacct gctgggtggc gcggaagcgc
     2821 tcggccagca ggaagatcag ggcggcatcg atgttgtcga tgctggctcg caggcgcagc
     2881 agctcggcgc gggcggcttc gtcggacatg atcctcctca gtggtgcggc gcgggtgcga
     2941 cagttcgacg atagtcggcg ggctctgctc ggatcgcgtg aattgcggca tccggtgacg
     3001 gattcgcgca cgtcacgcga ttcgagggct gcgtgcgcac atagagtgtt cggcatgagc
     3061 gccgacgagt ccgtgaagtc agagcccacc gcagagtcgg caacggtgca ggccgtcgtc
     3121 gtcgaggatg ccgcgccttc ccccgccccg ccggtggtcg agccgccgct gccgagccgc
     3181 tcgttctgga cgcgcatcga caagccgttc gcgttcggat tcctggtcgc cctgggcggc
     3241 ctcgccgcga tcctgctcgc actcacgctc tcgaacctgt ccaccgtgct gatctacatc
     3301 gcgttcgcga tgttcgcggc gctcggcctg gaccctgcgg tgcgattcct cgagcgacgc
     3361 ggtctctcgc gcggcgtcag cgtgctcgcg gtgatcctgg ggttggtcgt cgtgctggcg
     3421 ctgatcctgt ggatgatcat tcccgtagtg gtcgagcagg tcacgctgtt cgtcaagtcc
     3481 gtgccgcatc tgatcaccga cttcatgcac agcgatgtgt acgcgttcct ggagaagcag
     3541 ttcggcgacc agttccagca gctcgtcgag gacgcgcaga agttcctcac cgacggcaag
     3601 acgtggcagg ccgtcggcgg cggcgctctg caggtgggca gctccatcgc gaccggcatc
     3661 tcgggcttca tcgtggtgct ggtgctgacg ctgtacttcg tcgcaggcct ccccacgatc
     3721 aagaccgcgc tgctgcgctt cgcgccggcc cgcgaccgcg agcgggcccg cggcatcacc
     3781 gaccagatca cggactccgt cggcggctac gtgcagggca tggtgatcct ggcgttcgcg
     3841 aacgccatcg tcgcgctcgt gctgtacctg gccctcggcc tgccgttccc gcccctgatg
     3901 gcgacggtcg cgttcctggt cacgctgatc ccactggtgg gatcggtgct gttctgggcc
     3961 atcggctcgg tgatcgccct gttcgccgac ccgtggcacg cgctgatctt cgcggccgtc
     4021 tacctggtct acatgcaggt cgaggcgtac ttcctcacgc cccgcgtgat gaacaaggcg
     4081 gtcgcggtgc cgggggcgct cgtggtgatc ggcgcactgg ccggcggaac gctgctcggc
     4141 ctgctgggcg ccctcgtcgc ggtgccggtc acggcatcca tcctgatcat catcaatcag
     4201 gtgtggctgc cgcggcagga tgcccgggtc tgagcatccc gccgcggcag accggactca
     4261 gtcgagcccc ttcgccttga gccacttggc ggcgatgtcc tcggccgagg cctgatccac
     4321 cgtcgactgc acgttcatcg acacgaggtc ctcggcgccg agcgcggcgc tgatcgggtt
     4381 gatgaccttg gcgatcttgt cggcgatgtc gctggacgcg atgggcacga cgttcgaggc
     4441 caggatcatg ttcttcggat ccttcagcgc cacgatgtcc ttggtctgga aggcgggatc
     4501 ggcggtgtag atgtcggcca cctgcacctg acccgccagc agcgactcga gcgtgctctg
     4561 cgccgtggcg gagaacccga ggtcgacatc gtagatcttc ttcgcgccgg cggggccgta
     4621 cggacgctgc tccagctcgg gcgcgccgcc gagcacgggc ttcgtgttca gcttcgccag
     4681 gtcggcgatc gactcgaggc cgttgtcctg tgcgaacttc ttcgtgaccg tgtaggtgtc
     4741 ctggtcgctg gccttggagt agtccagcgc cttcagggtc ttcggcagcg ccttctgcag
     4801 tgccgcgtag acgtcgtcgg ggctggtggc ggtggcgctc ttgtcgaggt actccagcag
     4861 gttgccggtg tactcgggga agaggttgat cttgcccgac tccacgtccg gcatgtaggc
     4921 gtcgcgctgg ccgacgttga acttcttctc gaccgtgaag ccggcgttct ccagcgcctg
     4981 cgcgtagatc tcggcgatga tctcgttgga gtagtacgcc tgtgagccga cgacgatggt
     5041 ctcgccaccc ttgtctgtgc cgccgccggt ggagggctca tcgagcgggt tgctgctgcc
     5101 gcatccggcg agcgccaggg tcgcggcgag cgcgacgccg acggcgagtg cgagtcggga
     5161 cttccgtgcg gtgatcatga tgtgcctctc tgtggagtgg ttcaagcggt tcaggccgtg
     5221 acggatgccg cgcgtcgcgc ggaccgccgc ccattgcggt cggaggaaga ggtgcggtcg
     5281 ggggacgggg agcggatgcc ggtcggcaca gccgcgcgct gcagcgcggc cagcagcagg
     5341 tcgaccagca gcgcgagcac ggcgacgaga agcgcaccgg cgagcacctg gtcgaatcgc
     5401 cgcagcggga tgccctggat gatcggccag ccgagcccgc cgaggttcac gtacgcgccg
     5461 atcgtgacgg tcgcgatcac ctgcagcagg gcggagcgga tgccgccgac cagcagcggc
     5521 atgccgagcg ggagctcgac cttcgtgaag atctgccact cggtcatgcc gacggcacgt
     5581 gcggcgtcga tcaccctgcg atcgatggcc tcgaagcccg tgtatgcgcc cgcgagcagc
     5641 gaggggatgg cgagcagcac gaacgtgatg accgcggcca gcggtgtctg cagcacgccc
     5701 agcagcagca cgagcaggat gaggagcccg aacgacggca cggcgcgcgc ggcgccggag
     5761 acggccacgg cgatctcgcg cccctttccg gtgtgcccga tcagccagcc ggcggggagc
     5821 gcgatggcgg cggccaccag cacggagatc gcggtgtaca gcaggtgctg gccgagcagg
     5881 gtgggcaggg cgtactgccc ctgccactgc gcggggttca gcagccaggc gatcgcgtcg
     5941 gtgaagaggt tcatgcggcc gacctcacca ctgcgcgccg ggccttcgac gggctgaggg
     6001 cgccgccgcc cgccgctgtc gcccgcgtcc acggcatgag catccggccc aggagcacca
     6061 gcagcacgtc gacgacgaga gcgatcacga ccaccgcgac cacgccggcg agcacctcgg
     6121 cgatgatgcg ccgctgcagg ccgttggtga acaggtagcc caggttctcg acgccgatca
     6181 ggattccgac agtggcgagc gagatggtgc tgaccgctgc cacgcgcaga cccgcgagga
     6241 tcaccgggcc ggcgagcggc agctcgaccg tgaagaatct gcgcggggcg gcgtagccca
     6301 tggccgtggc cgcctgccgc acgtcagggt cgaccgagtc gagcccgtcg gcgaccgcgc
     6361 gcacgaggat cgcgaccgcg tagatggtca gggcgatgat cagattcggc tcgtcggtgg
     6421 ccgagtaccc gaagagggcc ggcaggagga tcagcagcgc gagggagggg atcgtgtaca
     6481 gcaggcccgt gagcacgatg accggcccgc gcaccagccg gtagcgccag gccacccagc
     6541 ccagcgggac ggacaggagc aggcccagca cgatcggcgt cgcgctctgc ttgagatgca
     6601 cgagcgtgag gctgaggatc agatccaggt tgtcgccgac ccagttcacg tcaggtcccg
     6661 tccttgagca cgccctgggt gcgtccctcg gagtccacca cgaccgtgcc gcgcggggtc
     6721 tgcttgagcg agagcgcgcg acggccgcgc tcggcgccga tgaacgcctc gacgaagtca
     6781 tccgccgggt tctcgatgat ctcgctgggg ctgccgacct gcacgacccg tgcacccttg
     6841 tcgaggatga ccacctggtc gccgagcagg aacgcctcgt cgatgtcatg cgtgacgaag
     6901 acgaccgtct tgtcgagctc gacctgcagg cggatgagct cctgctgcag atcggcgcgc
     6961 acgatcgggt cgacggcgcc gaaaggctcg tccatcagca ggatgttggg gtcagccgcg
     7021 agtccgcggg cgacgcccac gcgctgctgc tggcctccgg agagctggct gggatagcgg
     7081 tcggcgagcg aggcatccag ccccaccgtg ctcagcagct cccgtgaccg gccgtgcgcc
     7141 tcggtgcggc tcacgccgtt cagcctcagc acagtggaga cgttgtcgat caccgtgaag
     7201 tgcggcatga gcccggagtt ctgcatcacg tacccgatgc tgcggcgcag ccgcacaggg
     7261 tcgcggtcga gcacgctctc gccgtcgatc gcgatggtgc ccgaggtcgg gtcgaccatg
     7321 cggttgatca tgcgcagcag cgtggtcttg ccgcatccgg acgacccgac gaagacagtc
     7381 gtcttgcggg agggcagcac gaggctgaag tcctcgacgg ccagcgtgcc gtcggggaag
     7441 cgcttcgaga ccgaagtgaa ctcgatcatc cgccgcggcc taccggccga cctgccgcag
     7501 cgcggtggcg gggaagcagt ggttgccttc gatcacggtt ccctcgagta ctgccttcat
     7561 gtgcgtacct cctggcgtcc tttcgagcgt aggcgacacc tccgacaacg cgcaggagtc
     7621 gcgggaactt cccggattcc cagcatccgc gccgtccgga gtcaggcgga ctggcggccg
     7681 tactcctcca gcagccgcag ccacacctcg ttcatggtcg gataggccgg cacggcgtgc
     7741 cagagccggc tgatcggcac ctcgccggtg acggcgatgg tggcggcctg gagcagctcg
     7801 gcgacgtcgg cgccgacgaa cgtgactccc agcagcacct cgcgctcggt gtcgacgatc
     7861 atgcgcgcct gcccctggta gtcgtccgca tgcaggcccg atccggccac ccaccccagg
     7921 tcgtagtcga tgacctcgat ctcgtgtccg gccgcgcggg cggcatccgc cgtcaggccg
     7981 accgaggcga cctccggctc ggagaagacc acctgcggca cggccgcgtg gtcggcggtc
     8041 gcgacgtgac gcccccaggg ctgatcggcg accacttcgc cgcgcgaacg cgccgcgatc
     8101 acgtcgcccg cggcacgcgc ctggtacttg ccctggtggg tcagcagcac ccggccgttc
     8161 acatcgccga ccgcgtacag ccagtcgctg ccgggcacgc gcagggtgtc gtcgacctcg
     8221 atgtagcggc cgggctcgag gccgaccgtc tccaggccga tgtcgccgct gcggggcgtg
     8281 cgcccggtcg cgacgagcac ctcggatgcc gtgatctcct cgccgtcgcc gaccgtgacc
     8341 accacgtggt cgccgtcgcg gcgcacggcg gaggtggcgg tgttcagccg cacatccacg
     8401 cccatgccgc gcagaccctc ggcgacccgt tccccggcga agggctccag gccgccgagc
     8461 aggccactgc gggcgatcag cgtgacggat gccccgaggc cggcgtacgc ggtggccatc
     8521 tccgccgcca cgacgcctcc gccgatgatc gccagtgact cgggcacatg atcgacgctg
     8581 gtcgcctcac ggctggtcca cggcttcgca tccgcgagcc cggggatgcc ggggatcgtc
     8641 gcatccgttc cggtgctgat cgcgacggcc tgcctggcgc gcagcacccg gccgccgacg
     8701 gtgacctcgc gttcgccggt gaggcggccg tggccgcgca ccaggtcgat gccggcaccc
     8761 ttcagccaat cgacctggcc gtcatccttc cagtgcgagg tgaaggagtc gcggcgggcg
     8821 agcacgtcgg cggggctcag cgttccgccg ctcacgccct tgacgtgctg cgccgcgcgc
     8881 agcgcctgga tcgggcgcag cagcgccttc gacggcatgc acgcccagta cgagcattca
     8941 ccgccgacca gctcgctctc cacgatcacg gcggtcagcc cgccctgcac ggcccgatcg
     9001 gccacgttct ctccgacagg tccgccgccc agcacgatga ggtcgtattc gtcgctcatt
     9061 gcttcagtct tgctcagcat ccgctcggtg gggccgtcgc ctcgcagatc tgcacaactt
     9121 cgtctggttc tgcggacaga tctgcagaat tgcgagggaa tcgggccctt gccgccgatc
     9181 ccgcccggga acatactgtg cacatggcga tccggggcgc gcccgtcgtc gactggacgg
     9241 gaagattcga tcagctcgtc gcgcgggagg ctctcctcga cgcggaggaa ctggatgacc
     9301 tcggtcaggc agcgtggttc accggtcatg acgacgtcag ccaccgcgcc tgggagcggg
     9361 ctcacctccg tcacctcgac gggggcgacc tcaccgcggc cgtgcgctgc gcgttctggc
     9421 tcggcttcac gctcagcgag cagggggagg atgttcgggc gcgcacctgg atgggccggc
     9481 tggtcgagct gtgcgtccgc ccggaagcgg atgctcatgc ggccgcctac cgtgaactgt
     9541 gcggcgccgt agcgcattac gcccaagggg atctgctcgg atcggtaccg ctctaccggc
     9601 gcgccgcagc gcaggccgcc gacgagggcg acggcgatct ggaggccctc gccacgatgg
     9661 ggctgggccg tgcgctcgtg cgcctcggcg aggtcgagga ggggttcggc tgcctggacc
     9721 gggtgatgct catgatcggt tccgaccggg tcagcgatcg agccgcagga cccgcgttct
     9781 gcgccgtcgt ggcgagcctg ctcgcccgcg gcgatctgga gcgggcgagg gtgtggacgc
     9841 gcgatctggg cgactggtgc gacacgcagc aggggctgga gccgttccgc ggcgagtgca
     9901 ccctgcatcg cgcgacggtg atgcagctcg gcggcgagtg gggcgaagcc gcgaaggccg
     9961 ccgatcaggt ctgccgcacc gagaagcgca tcgagaccct cggcaacgcc tggtatcgag
    10021 cagcggagct gcatcgcgtc gcgggccgcg ccacccaggc ccgggacgcg taccggagag
    10081 cgacggggct cgggcgcgag gcgcaac
//
LOCUS       DATMSX010000269         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_108388,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000269 DATMSX010000000
VERSION     DATMSX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108388"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN041_08155"
     CDS             complement(<1..255)
                     /locus_tag="VN041_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29049.1"
                     /translation="MKWSDAAIERLNDARLCPLCEMSHLVQGRCAYCGADLSGPQGVA
                     LWNASTVAAESLRAREALLATVPRLVPVPTAVGVIAPPMAE"
     gene            complement(252..467)
                     /locus_tag="VN041_08160"
     CDS             complement(252..467)
                     /locus_tag="VN041_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29050.1"
                     /translation="MPIVIDIDVMLARRKMSVQEFADAIGITPANVAVLKNGRAKAVR
                     FTTLDAICRVLECQPGDILRYEPRGRS"
     gene            complement(467..943)
                     /locus_tag="VN041_08165"
     CDS             complement(467..943)
                     /locus_tag="VN041_08165"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HWU29051.1"
                     /translation="MQPVATLLLRAMIAVLIALLVFVQVVMIPGVADETARRNPGLAY
                     LEVPGIIGAVLFLALVQIVLVCVFALLSLVRADRIFSPHSFRWVDIIIGTMLAAAALL
                     VASFIVLAKDGAVNPSILVLAVFGVVVSLSLALLVSVMRGLLRKALQLEQDLSEVV"
     gene            complement(1076..1723)
                     /locus_tag="VN041_08170"
     CDS             complement(1076..1723)
                     /locus_tag="VN041_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU29052.1"
                     /translation="MERSLETQVGQAVDAWLRWVPRWEPATHRGRVAPCRRCLGSPIL
                     SAAGIGGNVPHGVQHGLSTRIKAIVDHAVAEYTARNLPMLQAELDHQAARNRSRTYRP
                     AEGLDPEYEGMPLDPDPVPGAPFLFTVAGLADEASAELPPLPPLSDEAKAALRQEVAL
                     ADEYANMIGREICGILLRHRLSVQAAISQYVEPQIEAMLAELSEALDSPFDPDTP"
     gene            complement(1848..2342)
                     /locus_tag="VN041_08175"
     CDS             complement(1848..2342)
                     /locus_tag="VN041_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29053.1"
                     /translation="MTTTGDPHGTPPQGVSASLSLVIGFVSFAALCILGLGMLSFFAD
                     IDILAVPGLDCWPGIVGMLGAIGAFSWMLWPTLSRGRASFLAVPSVALVTAVAHLVLV
                     WLSALLSGAGTDSALEAVVQLISRGSSAVLLGAALIAAWIAIALRRTKAAAPHWPWER
                     TDEE"
     gene            complement(2382..>2963)
                     /locus_tag="VN041_08180"
     CDS             complement(2382..>2963)
                     /locus_tag="VN041_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HWU29054.1"
                     /translation="APPAAPQPIFVQAPEPPRELGNRGAAGGIGLIAAVVFAILYLGA
                     ILGLGALAGDVTGENIATAALEPLTTWAFWVPVVVFWLAFWLLGAFVNRARWGKWVIL
                     GLLVAAATYGGHILGQLFQAPFWSIAPSKSAELVNEQLLAPLAIAAFLFARELTIWFG
                     AWVARSGAKKKALNAEAQAEYERTLEAGPSALR"
BASE COUNT          494 a         1031 c         1053 g          385 t
ORIGIN      
        1 ctcggccatc gggggcgcga tgacaccgac cgcggtcggc accggcacca gcctcggaac
       61 ggtcgcgagc agcgcctccc gagcacgcag cgattccgcc gccaccgtcg acgcattcca
      121 gagcgcgacg ccctgcggcc cgctcagatc ggccccgcag tacgcgcagc gcccctgcac
      181 gagatgcgac atctcgcaca gcgggcagag ccgcgcatcg ttcaaccgct ctatcgcggc
      241 atccgaccac ttcatgagcg ccccctcggc tcgtatctca gaatgtcgcc gggctggcac
      301 tccagcaccc ggcagatcgc gtccagcgtg gtgaagcgca cggccttggc ccgcccgttc
      361 ttcagcaccg ccacgttcgc cggggtgatg ccgatggcat cggcgaactc ctgcacgctc
      421 atcttgcgac gggcgagcat cacgtcgatg tcgatcacga tgggcatcag accacctcgg
      481 acagatcctg ctcgagctgc agcgccttgc gcagcaggcc gcgcatcacg ctgaccagca
      541 gcgccagcga gaggctgacc accacgccga acaccgcgag gaccaggatg ctggggttca
      601 cagcgccgtc cttcgccagc acgatgaacg aggcgacgag aagggcggcg gccgccagca
      661 tcgtgccgat gatgatgtcg acccagcgga aggagtgcgg gctgaagatg cggtcggcgc
      721 gcaccagcga cagcagggcg aacacgcaga ccaggacgat ctggaccagg gcgaggaaca
      781 gcactgctcc gatgatcccc gggacctcga gatacgcgag gccggggttg cggcgcgcgg
      841 tctcgtcggc gacgccgggg atcatgacca cctgcacgaa gaccagcagt gcgatcagca
      901 cggcgatcat cgccctgagc aggagtgtgg cgacgggctg catggtgctc ctatcgaaag
      961 acagcaacta tctatcgaaa aacgatagcc agcggatgca taggaactca agcgggtgcg
     1021 ggtgattccc agcatccgcg cggccgcacg tcgctgggcg ggtggcgacg ggtcgctacg
     1081 gggtgtcggg atcgaagggc gagtccagcg cctcgctgag ctcggccagc atcgcctcga
     1141 tctgcggctc gacgtactgt gagatcgccg cctgcacgct cagccggtgc cgcagcagga
     1201 tgccgcagat ctcccggccg atcatgttcg cgtactcgtc ggcgagcgcg acctcctggc
     1261 gcagagcggc tttcgcctca tcgctgagcg gaggcagggg cggcagttcc gcggacgcct
     1321 cgtcggcgag accggccacg gtgaacagga acggcgcccc cggcaccggg tcggggtcca
     1381 gcggcatccc ctcgtactcg gggtccaggc cctcggcggg acggtacgtg cgcgagcggt
     1441 tgcgggcggc ctgatgatcg agctcggcct gcagcatcgg caggttgcgc gccgtgtact
     1501 ccgcgacggc gtgatcgacg atcgccttga tgcgggtgga cagaccgtgc tgcaccccgt
     1561 gcgggacgtt cccgccgatc ccggcggcgg agaggatcgg cgaccccagg cagcggcggc
     1621 acggagcgac ccgcccgcga tgcgtggcgg gctcccagcg cggcacccag cgcagccagg
     1681 cgtcgactgc ctggcccacc tgggtttcca gtgaccgctc cacggttaca gggtacggcc
     1741 cggagcgctt cgcggcgcgg catccgggcg tgtcatccgt cgccgtccga cggcggatcc
     1801 ggcatcccga gttccggtgc ctcgcgcggt gaggccttcg tcacgcctca ttcctcgtcg
     1861 gtgcgctccc acggccagtg cggagcggcg gccttcgtgc ggcgcagggc gattgcgatc
     1921 caggcggcga tgagggccgc tcccagcagc acggcgctcg agccgcgcga gatcagctgc
     1981 acgacggcct cgagcgcgga gtccgtgccc gccccgctca gcagcgcgct gagccacacg
     2041 agcacgagat gcgcgaccgc ggtgaccagc gccacggagg gcaccgcgag gaacgatgcc
     2101 cgcccgcggg acagcgtcgg ccacagcatc caggagaagg cgccgatcgc accgagcatg
     2161 ccgacgatgc ccggccaaca gtccagcccc ggcaccgcga ggatgtcgat gtctgcgaag
     2221 aagctcagca tccccagacc caggatgcac agcgccgcga acgagacgaa gccgatcacc
     2281 agcgacagag acgcggagac cccctgcggc ggtgtgccgt gggggtctcc ggtggtggtc
     2341 atggcgccac cttacgcgcg tgcgcgacgg tggagggcgg ttcagcgcag cgcgctgggg
     2401 cctgcctcga gggtgcgctc gtactcggcc tgcgcctccg cgttcagcgc cttcttcttg
     2461 gcgccgctgc gggcgaccca tgcgccgaac cagatggtga gctcgcgggc gaacaggaag
     2521 gcggcgatcg cgagcggcgc gagcagctgc tcgttgacca gctctgccga cttgctgggc
     2581 gcgatgctcc agaacggcgc ctggaacagc tggccgagga tgtggccgcc gtaggtggct
     2641 gcggccacga gcaggcccag gatgacccac ttgccccagc gggcgcggtt gacgaaggcg
     2701 ccgagcagcc agaacgccag ccagaagacg accaccggaa cccagaaggc ccacgtggtc
     2761 agcggctcga gtgcggccgt cgcgatgttc tcaccggtga cgtcgcccgc gagggcgccg
     2821 aggcccagga tcgcaccgag gtacaggatg gcgaagacga cggccgcgat caggccgatg
     2881 ccgcctgcgg cgccgcggtt gcccagctcc cgaggcggtt ccggagcctg gacgaagatc
     2941 ggctgcgggg ctgcgggcgg ggc
//
LOCUS       DATMSX010000270         8073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_61110,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000270 DATMSX010000000
VERSION     DATMSX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61110"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VN041_08185"
     CDS             complement(<1..742)
                     /locus_tag="VN041_08185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWU29055.1"
                     /translation="MSSVTTSDRTADLHVSGFATLPSPSEVIGELPVDDLSVVVSRAR
                     DEVRAIMSGADDRLLVVVGPCSIHDTAAGLEYARRLASEAERHRDDLLIVMRTYFEKP
                     RTTVGWKGLINDPHLDGSHDIEAGLRLARGFLRDVTALGLPCATEFLEPISPQYTADL
                     IAWGAIGARTTESQIHRQLASGLSMPIGFKNGTDGGLQVALDAASAASAPQAFLGIGA
                     DGRASLVATTGNPDTSVILRGGADGPNFG"
     gene            complement(995..2467)
                     /locus_tag="VN041_08190"
     CDS             complement(995..2467)
                     /locus_tag="VN041_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWU29056.1"
                     /translation="MSTADTGTTPAQGASPGFIPAKDMAVIWVLLAAAFVAILNETTM
                     GMAIPHLITDLGITPIAAQWVTSAFMLTMAVVIPITGFLLRRFTTRQMFLSAIVLFSV
                     GTLLAALATGFPMLLGARVVQASGTAVMMPLLMTTIMNLVPAASRGRMMGRVSVVISL
                     APAIGPTMSGFLLDHFGWRAIFVVVLPIALVAMFIGWRWLTNVGETMHAPIDALSIVL
                     SAFGFGGLVFGLSQIGGMGNGASWMPMAIALAVGVVGLGLFLWRQVALQKEDDALLDL
                     RVFASRDFSLSMAQMFLLSLAFFGSITVIPLFLQDALQLKALDAGLVVLPGALAMGLL
                     GPVIGRIYDSHGTRILLIPGSILAAAMLWLFTTLSTGSSVWLVMVAQTLLSVGLALSF
                     TPLFTASLASLKPKFYSYGSAVIGTIQQVAGAAGIAVMMAVFTTIVNAGGGTEVPAAV
                     AAGTRTSITIGAVIATITIIGAFLIRKPEDDGEGPQHGGH"
     gene            2638..4641
                     /locus_tag="VN041_08195"
     CDS             2638..4641
                     /locus_tag="VN041_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29057.1"
                     /translation="MGGVRGGGGRGFRGVDEAAQRRLNAEAPRIADLGKRVVALFRPY
                     RGRIALTVVLVVLGAAIAVIPPLLVQRIFDDALFPVGGGEPVLGLLLRLVLTMIGLFL
                     LSAGLGVAQTWLTSTVGNSVTGDLRVRLFEHLQAMELGFFTRTKTGVIQSRLQNDVGG
                     VSGVLTNTITSILGNVVTVIASLVAMILIDWRLTLIAVVLMPLLVLVQRRVGQVRARI
                     AGQTQESLSELTAITQETLSVSGILLSKSFNRQRTESSRYQQENRNQVRLQVRQAMSG
                     QGFFAVVQVLMASIPAVIYLVSGYLVAGGTGAITAGTIVAFTTVQARLLMPMMGLMRV
                     ALDLQTSSALFARIFEYLDMVPEIQDAPDAIPVSQAPGPRGRVEFRDVVFRYPDAAPD
                     ARATLRGVSFVAEPGQQVAFVGPSGAGKTTILYLAPRMYAAASGEVLFGGADVRTLTQ
                     ESIIDDVGIVSQETYLFHATIRENLLYAKPEATEEELIAACTAANIHHIIAGFEHGYD
                     TVVGERGYRLSGGEKQRIAIARVLLKDPPVLLLDEATSALDTVSERVVQHALDQAAAG
                     RTVLTIAHRLSTVMGADMIHVIEAGRIVESGTHTELLAAGGLYTELAAQQMAASNVLS
                     DDTPALPERRADQAPEDSASEDAVRALTASVPVLDVPHLDPDA"
     gene            complement(4675..5448)
                     /locus_tag="VN041_08200"
     CDS             complement(4675..5448)
                     /locus_tag="VN041_08200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU29058.1"
                     /translation="MTDYLDSLFSLHGRIAVVTGGSSGIGRGIATALAKAGAATVIAA
                     RGRDHVEQTVQELTDAGCLAAGVIGDLSTREGIRKLAEAATEPFGEPDILVHSAGINI
                     RPPMTEITDDDWDATLTVNTHAPFLLGQRFAPGMAERGYGRIIHISSQQAHRAFVSSG
                     IYGVSKGAIESLMRSEAEAWGGTGVTSNTLVPGFVLTPLNARLQEDPAKVAELAARTM
                     IGRNGLPDDFAGAAVFLAGAGSGYVTGHSLFVDGGLSAH"
     gene            complement(5466..6404)
                     /locus_tag="VN041_08205"
     CDS             complement(5466..6404)
                     /locus_tag="VN041_08205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU29059.1"
                     /translation="MHPLVTMIVPGRDIASFAPAAIASLRAQTVPRWRALLIDDGSVD
                     DTGAVFADAAASDGRFEVLHHVEARGLGAARNIGLDRVDTPFVGFLDADDELTPSAIE
                     LWLRTLQESGSDFVAGAYVRNRANGAGYLPGRVQPWVAKATSPRRLGVTLAEHPDASA
                     NIVAWSKLSRTSLWDGLRFPENVAYEDQIVAQRMYTEARAFDVIPDVVAHWRLRADGT
                     SITQGKDRMPVLRDYLAALRGGIRVLHDAGHRAAVVARLDLILAMDLPPLRRIAAEHP
                     DPEYSAALMTFETELQELPEFADARPDPALAAALAW"
     gene            6474..7463
                     /locus_tag="VN041_08210"
     CDS             6474..7463
                     /locus_tag="VN041_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HWU29060.1"
                     /translation="MSSLTTEKTDWLLDSDPALRWQVQRDLLGDPADVWEATRDRIAT
                     AGFGAQILSHQDPDGQWAGGAYFPSRERDLTGLGIVERDEDAQPWIATAWALKDLRDL
                     GLNATALEGTAEKLAESSRWEYDDLPYWGGEVDVCINAFTLANGAWLGADVSALAAWF
                     PAHRLADGGWNCEAEEGNSVRSSFHSTLNAVVGMREYERLTGDTSLRATRHVGEEYLL
                     ERALMRRLSTGEAVAPWVSEFIAPSRWQYSALAALDHFRAAALLEGAAPDPRLAEAVE
                     LVRAARQPDGTWLQGEVPGGAVWVEIDAPQGEPSKWLTLIGTRVLDWWDASQH"
     gene            complement(7606..>8073)
                     /locus_tag="VN041_08215"
     CDS             complement(7606..>8073)
                     /locus_tag="VN041_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU29061.1"
                     /translation="GGQERGRRSGTENVAGAVALATALELAEAERARSSAAVMAATEA
                     FIAGVLRAVPQARLTGHPTRRLPGTASFTFEGISGESVLLELERRGVISSSGSACAAG
                     SDEPSHVLIACGIDPLVAQTSVRFTFGRTPLPDGAPAQLAALVAASVQAVAGP"
BASE COUNT         1245 a         2717 c         2935 g         1176 t
ORIGIN      
        1 cgccgaagtt cgggccgtcg gcgccgccgc gaaggatcac cgaggtgtcg gggttgccgg
       61 tcgtggcgac caggctcgcc cgcccatcgg cgccgatccc gaggaacgcc tgcggagcgg
      121 atgccgcgga tgcggcgtcc agggccacct gcagtccgcc gtccgtgccg ttcttgaagc
      181 cgatcggcat ggacagaccg gaggccagct gccggtggat ctggctctcg gtcgtgcgcg
      241 cgccgatcgc gccccaggcg atgaggtcgg cggtgtactg cgggctgatg ggttcgagga
      301 actccgtcgc gcagggcaga ccgagcgcgg tcacatcgcg caggaagccg cgggccaggc
      361 ggagccccgc ctcgatgtcg tggctgccgt cgagatgcgg atcgttgatc aggcccttcc
      421 agccgaccgt ggtgcgcggc ttctcgaagt aggtgcgcat gacgatgagc aggtcgtcgc
      481 ggtggcgttc cgcctcggat gccagtcggc gggcgtactc gagcccggca gccgtgtcgt
      541 ggatcgagca cgggccgacg acgacgagga gcctgtcgtc tgcgccggac atgatcgccc
      601 gcacctcgtc gcgggctcgg gagacgacga cggagaggtc gtcgaccgga agctcaccga
      661 tcacctcgga cggggagggg agggtggcga agccgctgac gtgcaggtcg gcggtgcggt
      721 cggatgtcgt gacgctgctc atgggtccag tcctctcgaa ggcggacccc gtgcagagcc
      781 cgccgaaacg gcacggggct tcgccccgaa atgacgaaag gcagagcgga tgctctgcct
      841 ttgccggctc tgaaggtgtc ggtgcgcgct acattcgcgc cggctcctcc agagccggcc
      901 caaaatacgc gaaccaacgg gtcatacccg gaacactacc gcacgcccgg ggaatccggg
      961 cgtgcggcag tgccatgtga cgacgcgacg gcggtcagtg cccgccgtgc tgcggcccct
     1021 cgccgtcgtc ctcgggcttg cggatcagga aggcgccgat gatcgtgatc gtggcgatga
     1081 ccgcgccgat cgtgatcgag gtgcgcgtgc ccgcggcgac ggccgccggc acctcggtgc
     1141 cgccgcccgc gttcacgatc gtggtgaaca cggccatcat caccgcgatg cccgcggcgc
     1201 ctgcgacctg ctggatggtg ccgatcaccg cgctcccgta ggagtagaac ttcggcttga
     1261 gcgaggccag cgacgcggtg aacagcggcg tgaacgacag ggccagtccc accgacagca
     1321 gcgtctgagc gaccatcacc agccagaccg agctgccggt gctgagcgtc gtgaacagcc
     1381 agagcatggc ggcggcgagg atcgaccccg ggatgagcag gatcctggtg ccgtggctgt
     1441 cgtagatgcg gccgatgacc gggcccagca gacccatcgc gagcgcaccg ggcagcacga
     1501 ccaggccggc gtcgagcgcc ttcagctgca gggcgtcctg caggaagagc gggatcaccg
     1561 tgatgctgcc gaagaaggcc agcgagagaa ggaacatctg cgccatggag agcgagaagt
     1621 cgcgcgacgc gaacacgcgc aggtcgagca gcgcgtcgtc ctccttctgc agcgccacct
     1681 ggcgccagag gaacaggccg agcccgacca cgccgacagc gagggcgatc gccatgggca
     1741 tccagctggc gccgttgccc atgccgccga tctggctgag accgaacacg aggccgccga
     1801 acccgaaggc cgagagcacg atcgacaggg cgtcgatcgg ggcgtgcatg gtctcgccga
     1861 cgttcgtcag ccagcgccat ccgatgaaca tcgcgaccag ggcgatcggc agcacgacca
     1921 cgaagatggc gcgccagccg aagtggtcga gcaggaagcc ggacatggtc ggcccgatcg
     1981 ccggagcgag ggagatgacc acgctcacgc gtcccatcat gcggccgcgg gaggcggcag
     2041 gcacgaggtt catgatcgtc gtcatcagga gcggcatcat gacggccgtg ccggatgcct
     2101 gcacgacgcg ggcgccgagc agcatcggga atccggtcgc cagcgctgcg agcagggtgc
     2161 cgacggagaa cagcacgatg gcactgagga acatctgacg ggtcgtgaaa cggcgcagca
     2221 ggaaccccgt gatcgggatg accacggcca tggtcagcat gaacgcgctg gtcacccact
     2281 gcgcggcgat cggcgtgatg ccgaggtcgg tgatcaggtg cgggatcgcc atgcccatcg
     2341 tcgtctcgtt gaggatggcg acgaaggcgg ctgcgagcag cacccagatg acggccatgt
     2401 ccttggccgg gatgaagccg ggggaagcgc cctgggcggg tgtggtgccg gtgtcggcgg
     2461 tggacatggg actcctcagg agatcttgat gtgcggtgtt ctcgcgagtg cgactgatca
     2521 gcgttacagc gtaaccggag ccaccgacat tccattccgg ttcgcaggat ctttccgatg
     2581 aacgcgccct gaacgggctt cgacgtcgga tgggcggatt agcctggggc gatgagcatg
     2641 ggtggcgttc gcggtggggg cggtcgcgga ttccgcggtg tcgacgaggc ggcgcagcga
     2701 aggctgaacg ccgaggcccc gaggatcgcc gacctcggga agcgcgtggt cgcactcttc
     2761 cgcccgtacc gcgggcgcat cgcactgacc gtcgtgctgg tggtgctggg cgccgcgatc
     2821 gccgtgatcc cgccgctgct ggtgcagcgg atcttcgacg acgcgctgtt cccggtcggc
     2881 ggcggcgagc ccgtgctcgg cctgctgctg cggctcgtgc tgaccatgat cgggctgttc
     2941 ctgctctcgg ccgggctcgg ggtcgcgcag acctggctca catccacggt cggcaacagc
     3001 gtcacgggcg atctgcgggt acggctgttc gagcatctgc aggcgatgga gctcggcttc
     3061 ttcacccgca ccaagaccgg tgtcatccag tcgcggctgc agaacgacgt cggcggggtg
     3121 tccggcgtgc tcaccaacac gatcaccagc atcctcggca acgtcgtgac cgtgatcgcc
     3181 tcgctcgtgg ccatgatcct gatcgactgg cggctgacgc tgatcgccgt cgtgctgatg
     3241 ccgctcctcg tgctggtgca gcgccgggtc ggtcaggtgc gcgcgcgcat cgccggtcag
     3301 acccaggagt cgctgtctga gctgaccgcg atcacacagg agaccctgag cgtgtcgggc
     3361 atcctgctgt cgaagtcgtt caacaggcag cgcaccgagt cctcgcgata ccagcaggag
     3421 aaccgcaacc aggtgcgcct gcaggtgcgg caggcgatga gcggccaggg cttcttcgcc
     3481 gtggtccagg tgctgatggc ctccatccca gccgtgatct acctggtgtc gggctacctg
     3541 gtcgcgggcg gcaccggcgc gatcacggcg ggaaccatcg tcgccttcac caccgtgcag
     3601 gcccgtctgc tcatgccgat gatgggcctg atgcgcgtcg cgctggacct gcagacgtcc
     3661 tccgcactgt tcgcgcgcat cttcgagtac ctcgacatgg tccccgagat ccaggacgcc
     3721 cccgatgcga tccccgtgtc gcaggcaccc ggcccgcggg gccgggtgga gttccgcgac
     3781 gtcgtcttcc gctacccgga tgccgcgccc gatgcgcgcg cgacgctgcg gggcgtctcg
     3841 ttcgtggccg agccggggca gcaggtcgcg ttcgtcgggc cctcgggagc cggcaagacc
     3901 acgatcctct atctggcccc gcggatgtac gccgcggcct ccggcgaggt cctgttcggc
     3961 ggcgcggatg tgcgcacgct cacccaggag tcgatcatcg acgacgtggg catcgtgtcg
     4021 caggagacct acctgttcca cgcgaccatc cgcgagaacc tgctctacgc caagcccgaa
     4081 gccaccgagg aggagctgat cgcggcctgc accgccgcga acatccacca catcatcgcg
     4141 ggcttcgagc acggctacga caccgtcgtc ggtgagcgtg gctaccggct ctccggcggc
     4201 gagaagcagc gcatcgccat cgcgcgcgtg ctgctgaagg acccgccggt gctgctgctg
     4261 gacgaggcga ccagcgccct ggacaccgtg tcggagcggg tggtgcagca cgcactggac
     4321 caggcggccg ccgggcggac cgtgctcacc atcgcgcatc ggctgtccac ggtgatgggc
     4381 gccgacatga tccacgtgat cgaggcaggc cgcatcgtcg agtccgggac gcacaccgag
     4441 ctgctggccg cgggcgggct gtacaccgag ctcgcggcgc agcagatggc cgcctcgaac
     4501 gtgctctcgg atgacactcc cgctctcccg gagcgacgcg ccgatcaggc gccggaggac
     4561 tcggcgtccg aggatgcggt gcgcgcgctc acagcatccg tcccggtgct cgacgtgcct
     4621 cacctcgacc ccgacgcata gcggcggccg gccggcgcgc gttcccgcat gccatcagtg
     4681 agcggagagg ccgccgtcca cgaacagcga gtgccccgtg acataccccg accccgcgcc
     4741 agccaggaac acggcggcac cggcgaagtc gtccggcagc ccgttgcggc cgatcatggt
     4801 gcgtgccgcg agttcagcga ccttcgcagg atcctcctgc agccgcgcgt tcaacggggt
     4861 cagcacgaag ccgggcacca gcgtgttgct cgtgacgccg gtcccgcccc acgcctcggc
     4921 ctccgagcgc atgagggatt cgatcgcgcc cttcgagacg ccgtagatgc cgctggacac
     4981 gaacgcgcga tgcgcctgct gcgagctgat gtggatgatc cgcccgtagc cgcgctcagc
     5041 catgcccggc gcgaaccgct gccccagcag gaacggggcg tgcgtgttca cggtgagggt
     5101 ggcatcccag tcgtcgtcgg tgatctcggt catcggcgga cggatgttga tgcccgccga
     5161 gtgcaccagg atgtccggct cgccgaacgg ctcggtggcc gcctccgcca gcttacggat
     5221 gccctcgcgg gtgctcagat cgccgatgac gccggcggcg aggcatccgg catccgtcag
     5281 ctcctgtacg gtctgctcca cgtgatcccg cccgcgggcc gcgatcacgg tcgccgcgcc
     5341 cgccttcgcg agtgcggtgg cgatgccccg gccgattccg gaggaaccgc ctgtgacgac
     5401 ggcgatgcgg ccgtgcagcg agaagagcga gtcgaggtag tcggtcacgg cgtctccgtt
     5461 cgggttcacc acgcgagtgc ggcggcgagg gcggggtcgg ggcgggcatc ggcgaactcc
     5521 ggaagctcct ggagctcggt ctcgaaggtc atcagcgccg ccgagtactc ggggtcggga
     5581 tgctcggcgg cgatgcgacg cagcggcggc agatccatgg cgaggatcag atcgaggcgg
     5641 gcgacgacgg cggcgcggtg accggcatcg tgcagcacgc ggatgccgcc gcggagcgcc
     5701 gcgaggtagt cgcggagcac cggcatccga tccttgccct gcgtgatcga ggtgccgtcg
     5761 gcgcgcagcc gccagtgcgc gacgacgtcg gggatcacat cgaacgcccg cgcctcggtg
     5821 tacatccgct gcgcgacgat ctggtcctca tacgcgacgt tctcgggaaa gcgcaggccg
     5881 tcccagaggc tcgtccggct gagcttggac caggccacga tgttcgcgga ggcatccgga
     5941 tgctcggcga gggtgacgcc gagacgccgc ggagaggtgg ccttcgccac ccacggctgg
     6001 acgcgccccg gcagatagcc cgcgccgttc gcccgattgc gcacgtatgc accggcgacg
     6061 aagtcgctgc cggattcctg gagagtccgc agccacagct cgatcgccga cggggtgagc
     6121 tcgtcgtcgg catcgaggaa ccccacgaac ggggtgtcga cgcgatccag cccgatgttg
     6181 cgggcggcgc ccaggccgcg cgcctcgacg tggtgcagca cttcgaaacg accatcggat
     6241 gccgccgcat ccgcgaacac ggctccggtg tcgtcgaccg atccgtcgtc gatgagcagc
     6301 gctcgccatc gcggcacggt ctgcgcccgc agcgatgcga tcgcggcggg tgcgaaggag
     6361 gcgatgtcgc gccccgggac gatcatggtg acgagcgggt gcacgacccc gatcctactt
     6421 ttcccggaga gggcgggctt ccggatgtca ggggtgcggc gtagcgtcgc agcatgtcat
     6481 cgctgacgac cgagaagacg gactggctgc tcgactccga cccggccctg cgctggcagg
     6541 tgcagcgcga cctgctcggc gatcctgccg atgtctggga ggcgacgcgc gaccggatcg
     6601 ccaccgcggg gttcggagcg cagatcctct cgcatcagga tccggacggg cagtgggccg
     6661 gaggggcgta cttcccgtcg cgcgagcgcg acctgaccgg gctggggatc gtcgagaggg
     6721 acgaggacgc gcagccctgg atcgcgacgg cctgggcgct gaaggacctg cgcgatctcg
     6781 gactcaacgc gacggcgctc gaggggacgg cggagaagct cgccgagagc agccgctggg
     6841 agtacgacga cctgccctac tggggcggcg aggtggatgt ctgcatcaac gccttcaccc
     6901 tggcgaacgg cgcctggctg ggcgcggacg tctctgcgct ggccgcctgg ttccccgcgc
     6961 accggctcgc cgacggcgga tggaactgtg aggcggagga ggggaactcg gtgcgctcgt
     7021 ccttccactc caccctcaac gcggtcgtcg ggatgcggga gtacgagcgt ctcaccggag
     7081 acacgtcact gagggcgacg cggcatgtgg gggaggagta tctgctcgag cgcgccctga
     7141 tgcgccggct ctccaccgga gaggccgtcg ccccgtgggt gagcgagttc atcgcgccca
     7201 gccgctggca gtacagcgcg ctcgccgcgc tggatcattt ccgcgctgca gcgctgctgg
     7261 agggagccgc tcccgacccg cgcctggccg aggccgtcga actggtccgc gcggcacggc
     7321 agccggacgg cacgtggctg cagggcgagg tgccgggagg cgcggtgtgg gtggagatcg
     7381 acgcaccgca gggcgagccc tcgaagtggc tcacgctgat cggcacgcgc gtgctcgact
     7441 ggtgggatgc ctcgcagcac tgagcgccac cgcgtgcgtg cgggcacagc cggattgccg
     7501 caaggtgcgc ggatgccgcg tgcacgtcgg gcggagaaat gtgctccgtg cgggtggtat
     7561 cgcgggattc gtccgcggga ggtgcacgcg cgggaagtgc tcggctcagg gtcccgcgac
     7621 ggcctggacg gatgctgcga ccagcgccgc gagctgtgcg ggcgcaccgt caggcagcgg
     7681 agtgcgtccg aaggtgaacc gcaccgaggt ctgcgccacc agcgggtcga tgccgcacgc
     7741 gatcaatacg tgcgagggtt cgtcggaccc cgctgcgcat gccgatccgc tggagctgat
     7801 cacgccgcgc cgttcgagct ccagaagcac ggactcgccg ctgattccct cgaacgtgaa
     7861 gctcgccgtg cccggcaggc gccgggtcgg atgccccgtc aggcgcgcct gcgggacggc
     7921 cctcagcacc ccggcgatga acgcctccgt cgccgccatg accgcggcgg atgaccgcgc
     7981 gcgctccgcc tcggcgagtt cgagggcagt cgccagggcg acggctcccg cgacgttctc
     8041 ggtgccgctc cgtcgccctc gctcctgtcc gcc
//
LOCUS       DATMSX010000271         7535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_22781,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000271 DATMSX010000000
VERSION     DATMSX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22781"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1523)
                     /locus_tag="VN041_08220"
     CDS             complement(129..1523)
                     /locus_tag="VN041_08220"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWU29062.1"
                     /translation="MSTMNITPTPPRNDRMLTVDRLRGMILEGEIDTVVLAFTDMQGR
                     LQGKLLHARFFLDSVLGHGTEGCNYLLAVDVDMNTVDGYDHASWASGYGDMEFIPDLS
                     TIRLMPHRPGSVLIQCDLTSTTGEAVRVSPRSMLRAQVDRAAGHGWKAVAGTELEFVI
                     YNTPYEKAWDDGYRNLIPANRYNIDYSILGSSIVEPLLRDIRNTMYSSGLTVESAKGE
                     CNFGQHEIAFLFDEVIATADNHSYYKTAAKDIARQHGQSITFMAKPNQREGNSCHVHM
                     SLRGKEDDGLVFWDEQNGRRSAVYDHFIAGVLATMREFTLFYAPNINSYKRFVKGSFA
                     PTSVAWGIDNRTTAVRLVGSGKSARMENRLPGGDMNPYLGLAAMLAGGLYGIENELSL
                     EEETRGNAYESGAPVVPTTMHAARDALANSEIAAQVFGEDVVRHYVHYADVEIEAFDA
                     AVTDWELTRGFERL"
     gene            complement(1520..2233)
                     /locus_tag="VN041_08225"
     CDS             complement(1520..2233)
                     /locus_tag="VN041_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWU29063.1"
                     /translation="MMDSLLVDAALHPVRGQMAFESCVEQLGSAIQLGIFRVGEKLPG
                     ERELAERLNVSRATLREAISALRAAGLVSTTRGRGGGTVVEPPKQAWPAGQHPPRRAE
                     IDDLIIFRSVIEPGAAHRAALTDLTDEQRQLLETGLAEVDAAETPADYRQADARLHLA
                     ISTVCGSDELAHACSGVQAKIHQLLAEIPFLKKNIEHADAEHREIVAAILDGDAERAR
                     RVMAAHCDATAALLKGLLK"
     gene            complement(2250..3797)
                     /locus_tag="VN041_08230"
     CDS             complement(2250..3797)
                     /locus_tag="VN041_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWU29064.1"
                     /translation="MSESTKAVGTGPVDDDAAALAELGYKQELHRGMSGFSNFAVSFS
                     IISILAGCITSFNIALKSGGPSAITIGWPLVGVFVLCVALAMAEVCSRYPTAGGLYFW
                     AGRLAKRSKRQWAWFVGWYNFLGEVAVTAAIDYGAAATMMAFANLMWGTEVTALNTFL
                     LFVALIVVHGLLNTFGVNLVSLLSSVSAWWHIIGVVIIVAVLWIMPSQHQDVAWTFTT
                     WHNETGWGFGPYVFLMGLLMAQYTYTGYDASAHVAEETKNASVAAPKGIVMSVLISII
                     GGWILLYTIVAAIQDGSEAGLSKIVGTDLGSGPAQIFMDALNNPDVAKFLLFIVCGAQ
                     FFCGMASVTANSRMSYAFSRDNAIPGSRLWAQVNKRTGTPTNSIWLCVVLSIVVTVPA
                     LFNTTAYAAVTSIAVIGLYIAYVTPVFLRRLNSAFQPGPWNLGRWSGLIGWVAVVWVV
                     FIVILFVLPPVTPITVDTFNYAPIAVAVVAILSIVLWYAYGKRDFMNSSAEAEHLTKS
                     ADTLLDE"
     gene            complement(3898..4800)
                     /gene="xerD"
                     /locus_tag="VN041_08235"
     CDS             complement(3898..4800)
                     /gene="xerD"
                     /locus_tag="VN041_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWU29065.1"
                     /translation="MQLDRALNAYLRHITIERGLSEHSIAAYRRDLSAYVDWLHGRGV
                     DATEGITQAVVAEFIGERSAADPPPAATSLARLQSSVRGWHRFLAREGIEEDDPTGRM
                     RPPKTPQRLPKALTIDQVERLLAAPSPEDPIGIRDRALLELLYATGARVSEAVSLDVD
                     DLSYGDVLRLRGKGDKERIVPVGSYARAAVDAYLTRVRPGLAAKGRASARLFLGARGA
                     PLSRQSAWLIIRAAAEKAQITAEVSPHTLRHSFATHLLQGGADVRVVQELLGHASVAT
                     TQIYTHVSVDTLRDVYATSHPRAT"
     gene            complement(4805..5392)
                     /locus_tag="VN041_08240"
     CDS             complement(4805..5392)
                     /locus_tag="VN041_08240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWU29066.1"
                     /translation="MTSPDPSREGLRDESFEPEVLSSEVGFRGAVWNVLDERVAYGDG
                     EIRRQYVAHTGAVAVVAIDDQDRVLLIQQYRHPIRHRDWELPAGLLDIPGEDPMVAAQ
                     RELAEEADITAEHWEHLVSSWTTPGGNDEMIHVYLATGIGAAAAAHAREDEEADIRVE
                     WVPLADAVEAVLEGRMHNGILSIGVLAAATKRDRR"
     gene            complement(5379..7070)
                     /locus_tag="VN041_08245"
     CDS             complement(5379..7070)
                     /locus_tag="VN041_08245"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWU29067.1"
                     /translation="MNSSVAGPKNDTTRHIFVTGGVVSSLGKGLTAASLGNLLTARGL
                     RVVMQKLDPYLNVDPGTMNPFEHGEVFVTDDGAETDLDIGHYERFLGIELSKAANVTT
                     GQIYSQVIAKERRGDYLGATVQVIPHITDEIKRRMRLQATEEPRPDVIITEVGGTVGD
                     IESQPFLESARQLRHELGRGNVFFVHVSLVPFMGASGEQKTKPTQHSVAALRQVGIQP
                     DALVLRSDRPVSESNRNKIALMCDVDTEGVVNTVDLPSIYDIPSTLNDQGLDAYIISK
                     LGLDERAGEVDWTRWNQVLHAVHNPKHEVTIGLVGKYIDLPDAYLSVTEALKAGGFAH
                     ETKVNIRWIASDTCETPEGAAKNLGDLDGILVPGGFGIRGIEGKLGALRFAREQGIPT
                     LGICLGLQCMVIEYGRHVAGIAGASSTEFDPETAEPIIATMAEQVEILDGGDLGGTMR
                     LGLYEAALAEGSLARELYGADTASERHRHRYEVNNSYRDRLTDAGLVFSGLNPDLDLV
                     EYVELPRDVHPYYIATQAHPELRSRPTAPHPLFRGLIGAAIERHRSSELFGDDES"
     gene            complement(7088..>7535)
                     /locus_tag="VN041_08250"
     CDS             complement(7088..>7535)
                     /locus_tag="VN041_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWU29068.1"
                     /translation="DVAILLAPHPGAEPRSVSRGASGGELSRVMLAIEVVIAGTDPVP
                     TFVFDEVDAGIGGAAAIEVGRRLAQLARTSQVIAVTHLAQVAAFATNHLSVVKSNDGS
                     VTASSVTRLSGEQREAEMARLLSGLADSDAALSHARELLSLGSGTN"
BASE COUNT         1157 a         2466 c         2682 g         1230 t
ORIGIN      
        1 gcgctcgatc gccgcatccg tctcggcgag atcggcctgc gggacggtgg cgacggcagc
       61 gccggtcgcg gggttcagca ccgtgaaggc gccgccggcg ccgaacgggg tggtggggat
      121 cctcatgctc acagcctctc gaatccgcgg gtcagctccc aatcggtgac ggcggcatcg
      181 aacgcctcga tctcgacgtc cgcgtagtgc acgtagtgcc ggacgacgtc ctcgccgaac
      241 acctgcgcgg cgatctcgga gttggccagt gcgtcacggg ccgcgtgcat ggtggtcggc
      301 acgaccggcg caccggactc gtaggcgttg ccgcgggtct cctcctcgag cgagagctcg
      361 ttctcgatgc cgtacaggcc gccggcgagc atcgcggcca ggcccaagta cgggttcatg
      421 tcgccgccgg ggaggcggtt ctccatccgc gcgctcttcc cgctgccgac cagccgtacc
      481 gcggtggtgc ggttgtcgat gccccaagcg acggaggtgg gcgcgaacga gcccttcacg
      541 aagcgcttgt aggagttgat gttcggcgcg tagaacagcg tgaactcgcg catggtcgcc
      601 aggacgcccg cgatgaagtg gtcgtacacc gcggagcgac ggccgttctg ctcgtcccag
      661 aagaccaggc cgtcgtcctc cttgccgcgc aacgacatgt gcacgtgaca ggagttgccc
      721 tcgcgctggt tcggcttggc catgaacgtg atcgactggc cgtgctgacg ggcgatgtcc
      781 ttcgccgcgg tcttgtagta ggagtggttg tcggcggtcg cgatcacctc gtcgaacagg
      841 aacgcgatct cgtgctggcc gaagttgcac tcccccttgg ccgactcgac ggtcagtccc
      901 gaggagtaca tggtgttgcg gatgtcgcgc agcagcggct cgacgatcga ggagcccagg
      961 atggagtagt cgatgttgta ccggttggcc gggatcaggt tgcggtagcc gtcgtcccac
     1021 gccttctcgt agggcgtgtt gtagatcacg aactccagct cggtgccggc gacggccttc
     1081 cagccgtgcc cggccgcgcg atcgacctga gcgcgcagca tgctgcgcgg cgacacccgc
     1141 accgcctcgc cggtcgtgct ggtcaggtcg cactggatca gcacgctgcc cgggcggtgc
     1201 ggcatcagcc ggatggtcga caggtcgggg atgaactcca tgtcgccgta gccgctcgcc
     1261 caggacgcat ggtcgtagcc gtcgaccgtg ttcatgtcga cgtcgacggc gagcaggtag
     1321 ttgcagccct ccgttccgtg gcccagcacg gagtcgagga agaagcgggc gtgcagcagc
     1381 ttgccctgca ggcggccctg catgtcggtg aaggcgagga cgaccgtgtc gatctcccct
     1441 tccaggatca ttccgcgcag gcggtccacg gtcagcatcc gatcgttgcg cggaggtgtg
     1501 ggagtgatgt tcatcgtgct catttcagaa ggcccttcag gagtgcggcc gtggcgtcgc
     1561 agtgcgccgc catgacacgg cgcgcgcgct cggcgtcgcc gtcgaggatc gccgcgacga
     1621 tctcccggtg ctcggcgtcg gcgtgttcga tgttcttctt gaggaatggg atctcggcga
     1681 gcagctggtg gatcttcgcc tgcacgccgc tgcacgcgtg cgcgagctcg tccgagccgc
     1741 agaccgtgct gatggcgagg tgaagcctgg catccgcctg ccggtagtcg gcgggcgtct
     1801 cggcggcgtc gacctcggcg aggccggtct ccagcagctg ccgctgctcg tcggtgaggt
     1861 cggtgagggc ggcgcggtgc gccgctcccg gttcgatcac cgagcggaag atgatcaggt
     1921 cgtcgatctc ggctcggcgg ggcggatgct ggccggcggg ccacgcctgc ttcggcggct
     1981 cgacgacggt gccccctccg cgcccccggg tcgtcgatac gagtccggcg gcacggaggg
     2041 cgctgatggc ctcccggagg gtggcgcgcg agacgttcag ccgctcggcg agctcgcgct
     2101 cgccgggcag cttctcgccc acccggaaga tgccgagctg gatggccgat ccgagctgct
     2161 ccacgcagga ttcgaaggcc atctgcccgc gcaccgggtg cagcgcggcg tcaaccagga
     2221 gggagtccat catcgtcgtc cgatcgcgct cactcgtcga gcagcgtgtc tgcggacttg
     2281 gtcaggtgct cggcctctgc gctgctgttc atgaagtcgc gcttgccgta ggcgtaccac
     2341 agaacgatcg acaggatggc gacgaccgcg acggcgatcg gcgcgtagtt gaacgtgtcg
     2401 accgtgatcg gggtgaccgg cggcagcacg aacaggatga cgatgaacac cacccagacc
     2461 accgcgaccc agccgatcag accgctccag cggccgaggt tccacgggcc gggctggaac
     2521 gcggagttca gccggcgcag gaacaccggg gtcacgtagg cgatgtacag gccgatcacc
     2581 gcgatggagg tgacggccgc atacgcggtc gtgttgaaca gggcggggac agtgaccacg
     2641 atcgacagca ccacgcacag ccagatcgag ttggtcgggg tgccggtgcg cttgttcacc
     2701 tgtgcccaca gcctcgatcc ggggatggcg ttgtcgcgcg agaacgcgta gctcatccgc
     2761 gagttggcgg tgaccgatgc catgccgcag aagaactggg cgccgcacac gatgaacagc
     2821 aggaacttcg cgacgtcggg gttgttcagc gcgtccatga agatctgcgc cgggcccgag
     2881 ccgagatcgg tgccgacgat cttcgacagt cccgcctcgc tgccgtcctg gatcgccgcg
     2941 acgatcgtgt acagcaggat ccagccgccg atgatcgaaa tcagcacgct catcacgatg
     3001 cccttcggcg ccgcgaccga ggcgttcttg gtctcctccg cgacgtgcgc cgacgcgtcg
     3061 tagccggtgt aggtgtactg ggccatcagc aggcccatca ggaacacgta gggaccgaag
     3121 ccccagccgg tctcgttgtg ccaggtggtg aaggtccagg cgacgtcctg gtgctgcgag
     3181 ggcatgatcc acagcacggc gacgatgatc acgacgccga tgatgtgcca ccacgccgac
     3241 accgaggaga gcaggctcac caggttcacg ccgaaggtgt tcagcagtcc gtggacgacg
     3301 atcagcgcca cgaacagcag gaaagtgttc agcgcggtga cctcggtccc ccacatcagg
     3361 ttggcgaagg ccatcatggt ggcggccgcg ccgtagtcga tggcggcggt caccgcgacc
     3421 tcgccgagga agttgtacca cccgacgaac cacgcccact ggcgcttgct cctcttggcc
     3481 aggcgtccgg cccagaagta caggccgccg gcggtgggat agcgggagca gacctccgcc
     3541 atggccagcg cgacgcacag cacgaagacg ccgaccagcg gccatccgat ggtgatcgcg
     3601 ctcggtcccc ccgacttcag cgcgatgttg aaggacgtga tgcaaccggc caggatcgag
     3661 atgatcgaga aggacaccgc gaagttcgag aagcccgaca tcccgcgatg gagttcctgc
     3721 ttgtagccga gttcggcgag ggcggcggcg tcatcatcga cgggaccggt ccccactgcc
     3781 tttgtggatt cgctcatgga agtgctacct gctttcagtg gagggagcgt ttggagcgat
     3841 tctggcctca tccaccgaaa gctgtcaatg gtctgacttc aatccattta cggtcttcta
     3901 agtcgctcgc ggatgcgagg tggcgtagac gtcgcgcagg gtgtcgaccg agacgtgggt
     3961 gtagatctgc gtcgtcgcga cggatgcatg ccccagcagc tcctgcacga cacgcacgtc
     4021 ggcgccgccc tgcaacaggt gcgtggcgaa ggagtgccgc agggtgtgcg gcgacacctc
     4081 cgcggtgatc tgcgccttct cggccgcggc gcggatgatc agccaggcgc tctgccgcga
     4141 cagcggagcg ccgcgcgcgc ccaggaacag tcgggcgctc gcccgccctt tggccgccag
     4201 tcctgggcgc accctggtca ggtacgcgtc gacggcggca cgggcgtagg agcccaccgg
     4261 cacgatgcgc tccttgtcgc ccttgccgcg caggcgcagc acatccccgt aggacaggtc
     4321 gtccacgtcc agcgacacgg cttccgacac gcgcgcgccg gtcgcgtaga gcagctcgag
     4381 cagagcgcga tcgcgaatgc cgatcggatc ctccggcgag ggcgccgcca gaagccgttc
     4441 gacctgatcg atcgtcagcg ccttcggcag cctctgcggg gtcttcgggg gacgcatccg
     4501 accggtggga tcgtcctcct cgatgccctc cctggcgagg aagcggtgcc agccccgcac
     4561 ggaggactgc agccgggcga gactggtcgc cgccggcggc ggatcggccg ccgagcgctc
     4621 gccgatgaac tccgccacca cggcctgagt gatgccctcg gtcgcgtcga ccccgcgccc
     4681 atggagccag tcgacgtacg cgctcaggtc gcggcggtag gcggcgatcg agtgctcgga
     4741 gaggccgcgc tcgatcgtga tatgtcgcag gtaggcgttc agcgcacggt cgagctgcat
     4801 gtcgtcagcg gcggtcgcgc ttcgtcgcag ccgcgagcac gccgatggag aggatgccgt
     4861 tgtgcatccg tccctcgagc acggcctcca cggcatccgc cagcggcacc cactccacgc
     4921 ggatgtcggc ctcctcatcc tcccgggcgt gcgcggcggc ggccgcgccg atgccggtgg
     4981 cgaggtagac gtggatcatc tcgtcgttgc cgcccggagt cgtccagctc gagaccaggt
     5041 gctcccagtg ctcggcggtg atgtcggcct cctccgccaa ctcgcgctgc gccgcgacca
     5101 tggggtcctc ccccgggatg tcgagcaggc cggcgggcag ttcccagtcg cggtgccgga
     5161 tcggatgccg gtactgctgg atcagcagca cacggtcctg atcgtcgatc gcgacgacgg
     5221 cgacggcgcc ggtgtgcgcg acgtactggc ggcggatctc accgtcgccg tacgccacgc
     5281 gctcatcgag cacgttccag acggctcccc ggaacccgac ctcactggag aggacctcgg
     5341 gctcgaagga ctcgtcccgc aggccctctc gcgaaggatc aggactcgtc atcgccgaac
     5401 agctcgctcg agcggtgccg ctcgatggcg gcgccgatca ggccgcggaa cagcgggtgc
     5461 ggcgcggtcg ggcgcgagcg cagctcgggg tgcgcctgcg tggcgatgta gtacgggtgc
     5521 acatcgcgcg ggagctcgac gtactcgacc aggtcgaggt cggggttcag acccgagaac
     5581 accagtccgg catcggtgag gcggtcgcgg taggagttgt tcacctcgta gcggtggcgg
     5641 tggcgctcgg aggccgtgtc cgcaccgtac agctcgcgcg ccagggagcc ctcggcgagg
     5701 gcggcctcgt acaggcccag gcgcatggtg ccgcccaggt cgccgccgtc gaggatctcg
     5761 acctgctcgg ccatggtcgc gatgatgggc tcggcggtct cggggtcgaa ctcggtcgac
     5821 gacgcccccg cgatgcctgc gacgtggcgg ccgtactcga tgaccatgca ctgcaggccc
     5881 aggcagatgc cgagcgtggg gatgccctgc tcacgcgcga agcgcagcgc gcccagcttg
     5941 ccctcgatgc cccggatgcc gaagccgccc ggcacgagga tgccgtccag gtcgccgagg
     6001 ttcttcgcgg cgccttccgg cgtctcgcag gtgtccgagg cgatccagcg gatgttgacc
     6061 ttggtctcgt gagcgaagcc gccggccttg agcgcctcgg tcacggacag gtacgcgtcg
     6121 ggcaggtcga tgtacttgcc gactaggccg atggtgacct cgtgcttggg gttgtgcacg
     6181 gcgtgcagaa cctggttcca gcgggtccag tcgacctcgc cggcccgctc gtccaggccc
     6241 agcttggaga tgatgtacgc gtccaggccc tggtcgttca gggtggaggg gatgtcgtag
     6301 atgctcggca gatcgacggt gttgacgacg ccctcggtgt cgacgtcgca catcagggcg
     6361 atcttgttgc ggttgctctc gctcacaggc cggtcgctgc gcagcacgag ggcgtcgggc
     6421 tggatgccga cctggcgcaa ggcagcgacg gagtgctggg tcggcttggt cttctgctcg
     6481 cctgaggcgc ccatgaaggg gaccagcgag acgtgcacga agaacacgtt gccgcgaccg
     6541 agctcgtggc gcagctgccg cgcggactcg aggaacggct gcgactcgat gtcaccgacg
     6601 gtgccgccga cctcggtgat gatcacgtcg gggcgcggct cctcggtggc ctgcaggcgc
     6661 atgcggcgct tgatctcgtc ggtgatgtgc ggaatcacct gcaccgtggc gccgaggtag
     6721 tcgcctcggc gctccttggc gatcacctgc gagtagatct ggccggtcgt gacgttggcg
     6781 gccttcgaca gctcgatgcc gaggaagcgc tcgtagtgac cgatgtcgag gtcggtctcc
     6841 gcgccgtcgt cggtgacgaa cacctcgccg tgctcgaacg ggttcatggt gcccgggtcc
     6901 acgttcaggt acgggtcgag cttctgcatg accacgcgca gtccgcgggc ggtgaggagg
     6961 ttgcccaggc tcgccgccgt gagacccttc cccaacgacg aaacgacacc gcctgtcacg
     7021 aagatgtgtc gagtggtgtc gttcttgggc cccgcaacag aagagttcat cacgggcttt
     7081 tatcctatca gttcgttccc gagccgaggc tcaggagttc acgggcgtgg ctgagagcgg
     7141 catccgaatc ggcgagcccg gagagcagtc gcgccatctc cgcctcgcgc tgctcacccg
     7201 agagacgggt gacgctggag gccgtgacgg agccgtcgtt cgacttgacc acggacaggt
     7261 ggttggtggc gaaggccgcg acctgggcga ggtgggtgac cgcgatcacc tgcgaggtgc
     7321 gggcgagctg ggcgagcctg cggccgacct cgatggcggc cgcgccgccg atgccggcgt
     7381 cgacctcgtc gaacacgaag gtcgggaccg ggtcggtgcc ggcgatgacg acctcgatgg
     7441 ccagcatgac gcgggacagc tcgccgcccg aggctccgcg cgacacggag cgcggctcgg
     7501 cgccggggtg cggggcgagc aggatcgcga cgtcg
//
LOCUS       DATMSX010000272         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_108394,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000272 DATMSX010000000
VERSION     DATMSX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108394"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="hisD"
                     /locus_tag="VN041_08255"
     CDS             <1..1083
                     /gene="hisD"
                     /locus_tag="VN041_08255"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWU29069.1"
                     /translation="RAALETAIERVRRGSAAQVPAPQTTDIGPGARITQRWQPITRAG
                     VYIPGGKAPLASSVVMNVVPAQVAGVTSIALASPPQAGHDGRVHPTILAAAALLKVEE
                     VYAIGGAGAIGALAYGVPELGLDPVDVVTGPGNNYVASAKRAVAGVVGTDSEAGATEI
                     LIVADEDADADLVAADLISQAEHDEQASAVLVTDSAALAERVSERVAARAAATRHAER
                     VAAALSGPQSAIVLVDDRRMAESFSNAYAPEHLELHLVDAAQAAGRFTSAGAVFVGAQ
                     TPVSLGDYMAGSNHVLPTGGQARYASGLGAYTFLRPQQVIEYDHDALARVRSGVVALA
                     DAEILPAHGEAVEARFAGAPDAERAE"
     gene            1095..1571
                     /gene="nrdR"
                     /locus_tag="VN041_08260"
     CDS             1095..1571
                     /gene="nrdR"
                     /locus_tag="VN041_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWU29070.1"
                     /translation="MHCPFCRHPDSRVIDSRTSDDGLSIRRRRQCPECGGRFSTTETA
                     SLVVIKRSGVMEAFSRDKVISGVRKACQGRPVTEADLAVLAQKVEEAIRQTGVSQLET
                     NEIGLSILGPLRELDEVAYLRFASVYQEFDSLEDFERSISELRADHAKAGTDAADR"
     gene            1582..2601
                     /locus_tag="VN041_08265"
     CDS             1582..2601
                     /locus_tag="VN041_08265"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWU29071.1"
                     /translation="MYPLLFRHVLSRFDPEFAHHAAMAVIRVLGVPPFSWATRALTAP
                     SPEQKVHALGLTFPSPFGVAAGFDKNVVGVRGLDALGFGHIEVGTVTAIPQDGNPKPR
                     LFRLVPDRAVVNRMGFNNNGAEAAEARLMRLRRRAPRAVIGVNIGKSRVVAVEDAIAD
                     YVASATRLAPLADYLAVNVSSPNTPGLRGLQAVETLAPLLRAVKEAAGSTPLLVKIAP
                     DLPDDEIAAIARLAVDEGLAGIIAHNTTISREGLRTDPSVVEAAGAGGLSGAPLKERS
                     LEVLAVVKAAVPADFCVIAVGGVETADDVQKRLDAGATLVQGYTAFLYRGPFWARQIN
                     RGLRR"
     gene            complement(2634..>3115)
                     /locus_tag="VN041_08270"
     CDS             complement(2634..>3115)
                     /locus_tag="VN041_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HWU29072.1"
                     /translation="EREAANRRPLVANTKEARAAAKADLQARREKARIGMEQGDDKYL
                     PARDKGPQRRWVRDYVDAGWHISEWLMAAMLVVILLNLTPIADVQLWAMVALWGYMLL
                     AIGDMVLLSIRVKKKVADKFGASRREKGLGWYAAMRALQMRFMRLPKPQVKRGQYPA"
BASE COUNT          443 a         1136 c         1078 g          458 t
ORIGIN      
        1 agggccgcgc tggagaccgc gatcgagcga gtccgccgcg gatcggctgc ccaggttccc
       61 gcgccgcaga ccaccgacat cgggccgggc gcgcgcatca ctcagcgctg gcagccgatc
      121 acccgcgccg gtgtctacat ccccggcggc aaggcacctc tcgcctccag cgtcgtcatg
      181 aacgtcgtgc ccgcccaggt cgccggcgtc acgagcatcg cgctggcctc gcccccgcag
      241 gcggggcacg acggccgcgt gcacccgacg atcctcgccg ccgccgcgct gctgaaggtc
      301 gaggaggtct acgcgatcgg tggtgccggt gccatcggcg cgctcgccta cggcgtccct
      361 gagctcggtc tcgaccccgt ggacgtcgtg accggccccg gtaacaacta cgtcgcctcg
      421 gccaaacgcg ccgtcgcggg agtcgtcggc acggactccg aggcgggcgc cacggagatc
      481 ctcatcgtcg cggacgagga cgcggatgcc gatctcgtcg cggccgacct catcagccag
      541 gccgagcacg acgagcaggc ctccgccgtg ctcgtgaccg actccgccgc cctcgccgag
      601 cgcgtctccg agcgggtcgc cgcacgtgca gcggccacac gccacgccga gcgcgtggcc
      661 gcggcgctga gcggaccgca gtccgctatc gtcctggtcg acgatcgccg catggcggag
      721 tcgttcagca acgcctacgc tccggagcac ctcgaactgc acctggtcga cgctgcccag
      781 gcggcaggcc ggttcaccag cgccggcgcc gtcttcgtcg gggcgcagac gccggtcagc
      841 ctcggagact acatggccgg cagcaaccac gtcctgccga ccgggggcca ggcgcggtac
      901 gcgtcggggc tgggcgccta caccttcctc cgtccgcagc aggtgatcga gtacgaccac
      961 gatgcgctcg cacgggtccg ctccggagtc gtcgcgctgg cggacgccga gatcctgccc
     1021 gcgcacggcg aggccgtcga ggcgcgattc gccggggcgc ccgacgcgga gcgcgccgag
     1081 taggctggaa catcatgcac tgccccttct gccggcatcc ggattcccgc gtcatcgact
     1141 cgcgcacgag cgatgacggt ctgtcgatcc gtcgccggcg gcagtgcccg gagtgcgggg
     1201 gccgcttctc gacgaccgag accgcgagcc tggtggtgat caagcgctcc ggtgtcatgg
     1261 aggcgttcag ccgggacaag gtgatctccg gcgtgcgcaa ggcctgtcag ggccgcccgg
     1321 tgaccgaggc cgacctcgcc gttctcgcgc agaaggtcga ggaggccatc cgccagaccg
     1381 gggtgtcgca gctggagacg aacgagatcg gcctgtcgat cctcgggccg ctgcgcgagc
     1441 tcgacgaggt cgcctacctg cgattcgcca gcgtgtacca ggagttcgac tcgctggagg
     1501 acttcgagcg ctccatcagc gagctgcgcg ccgaccacgc caaggcggga acggacgccg
     1561 cggaccggta atctggcagg gatgtatccg ctgctcttcc gccacgtcct gtcgcgcttc
     1621 gatccggagt tcgctcacca cgccgccatg gcggtcatcc gcgtgctcgg cgtcccgccg
     1681 ttctcctggg cgacccgcgc gctgacagcg ccgtcgccgg agcagaaggt gcacgcgctc
     1741 ggcctgacct tcccctcgcc gttcggcgtg gcagcaggct tcgacaagaa cgtcgtgggt
     1801 gtgcgcggcc tggacgcgct gggcttcggt cacatcgagg tcggcactgt gaccgcgatc
     1861 ccgcaggacg ggaacccgaa gccgcggctg ttccgcctcg tgcccgatcg cgcggtcgtc
     1921 aatcggatgg gcttcaacaa caacggtgcg gaggccgcgg aagcgcggct gatgaggctt
     1981 cgtcgtcgcg ccccgcgtgc ggtgatcggc gtgaacatcg gcaagagccg cgtggtcgcc
     2041 gtcgaggacg cgatcgccga ctacgtcgca tccgccacac gcctggcacc gctcgccgac
     2101 tacctcgccg tcaacgtctc ctctccgaac acgcccggac tgcgcgggct gcaggccgtg
     2161 gagacactcg cgccactgct gcgcgcggtc aaggaggccg cgggctcgac gcccctgctg
     2221 gtgaagatcg cgcccgacct gcccgacgac gagatcgccg ccatcgcgcg gctcgccgtt
     2281 gacgagggac tcgccggcat catcgcgcac aacaccacga tctcccgcga agggcttcgc
     2341 accgatccgt ccgtcgtcga ggcggccgga gccggtggcc tctccggcgc gcccctgaag
     2401 gagcgctcgc tcgaggttct cgcggtcgtc aaggccgccg ttcccgcgga cttctgcgtg
     2461 atcgcggtcg gcggcgtgga gacggcggac gacgtgcaga agcggctgga cgccggtgcg
     2521 accctcgtgc agggctacac ggcgttcctg taccgcggtc cgttctgggc gcgccagatc
     2581 aacagaggcc tgcgccgctg agcggcacag gcctctgttg agagctcttc gcgtcaggcg
     2641 gggtactgac cgcgcttgac ctgcggcttg ggcaggcgca tgaaacgcat ctgcagagcg
     2701 cgcatcgcgg cgtaccagcc caggcccttc tcccggcgcg aggcgccgaa cttgtcggcg
     2761 accttcttct tcacgcggat gctgagcagc accatgtcac cgatggcgag gagcatgtag
     2821 ccccagagcg cgaccatggc ccacagctgc acgtcggcga tcggagtgag gttcagcagg
     2881 atgaccacca gcatcgccgc catcagccac tcggagatgt gccagccggc gtcgacgtaa
     2941 tcgcgcaccc agcggcgctg cggtcccttg tcgcgagcgg ggaggtactt gtcgtcgccc
     3001 tgctccatcc cgatgcgggc cttctcgcgg cgcgcctgca ggtcggcctt cgcagcagcc
     3061 ctggcctcct tggtgttcgc gacgaggggg cggcggttgg ccgcctcgcg ctcgg
//
LOCUS       DATMSX010000273         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_98809,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000273 DATMSX010000000
VERSION     DATMSX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98809"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VN041_08275"
     CDS             <1..1828
                     /locus_tag="VN041_08275"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HWU29073.1"
                     /translation="GRVTGDGERRESMETIGRSFLLASARIAGSPDGDVEADALIEFY
                     AQAIVDGTDPGHPSAWPRGVTCRTPLTGITNSIVEGANLAFGLYTAGDRFWSRLDDRQ
                     QGQVLAWLRHHALREVWQNNWQLFPAMAEAFIRHRGGDARGLHGERNVARVEGWYLGD
                     GWYTDGPSHAVDYYNAWAIHPYLWAWYRMTDRTQTYEGQRHLERLAQFVERMPSFVAS
                     DGALVHFGRSLTYRTAALAPLWCAEISGVSPLPPARTRQVATSVLDGFVRHGVGADGQ
                     LHLGWYDEHLPASLQDYSGFGSPYLAGIGFLGLALGAGHRVWTDAAEPAEHPASTCPL
                     DGTGITLSTAGGDAPVILVNHGSDHAELPVQDHGIRDPDDPHYGSFAYSTHTAPEVGD
                     GWLEAVDGHVCLFDDQGRPSRRAKLRGRMQDGPLSASVHYPQIDGRLLVGAAIVTASV
                     VNRGTEVRAHLVIAPKVHDRIREGAFALAADEPPEQDVASDRLHASVADGTHRVMLTG
                     LHGWDDAGVVTHRGTNAMGEHSAIPILTATRGEDETVHVAVHQLLRDRDDPDAALAGL
                     RVAVDGTQVRVEWPDDSVDVEFDLRTVVEWDGQPGRPGGRRN"
     gene            complement(2010..3455)
                     /locus_tag="VN041_08280"
     CDS             complement(2010..3455)
                     /locus_tag="VN041_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWU29074.1"
                     /translation="MTTSPWTERALPSDRQPIPLRFGTQVSHREDEAMQRFRRYGLGQ
                     FIHFGLYSILGGEYQGVSAAGGSAAPEWIRAWSGPTAPEGWQGIYDRLNESFDPAELD
                     AHAWARQAKRMGARYVIFTTKHHDGFALWPSAHSDYTVRESPCGRDLVGEVVDAYTAE
                     GIDVFLYFSVLDWHHPGYLRTVPATDAERAGWQDFLQYTRAQLLELLDRYPAARGLWF
                     DGTWDASWIASHEFTHALEAELRARRPGLIIGSRFRNDEHGARHFDSNGDLLGDYEQG
                     WERKLPASFEMLGGNDWDCVITIGPNAWGWLRDTRDQYQKTADDLIDLLMRCRSMNGN
                     LVVNFGPDGLGRMTSHEDEIAEQLGAWVAANADAVYGAHHVDLDRTGAGWLTGDGDRL
                     FVTVVNRPVTGILRMRFPAGAERMPVSARLLDSGRDLPVRVTDIGFDLDDALYCDVSI
                     PADLEAEHAFVIELALGEASASAGDRIDAWM"
     gene            complement(3493..>4000)
                     /locus_tag="VN041_08285"
     CDS             complement(3493..>4000)
                     /locus_tag="VN041_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWU29075.1"
                     /translation="LFAGGGYRPELPAGAEDGPLEVVALMDEAVMSRTARELLHRAHA
                     AVFTYAVLPDIVGHTEGVTDRYRRAVETCDEQLGVLLAAIDARENREHEDWTVIVLTD
                     HGHLDAGHHGGDSDEERLAWMAAAGPDVPSVEPVVDHADVLPHVLKALGLPADSALPG
                     APFGGRSR"
BASE COUNT          576 a         1419 c         1426 g          579 t
ORIGIN      
        1 ggggcgcgtg accggcgacg gggagcgccg cgagtcgatg gagaccatcg ggcgcagctt
       61 cctgctcgcc tccgcccgca tcgcgggctc acccgacggc gacgtcgagg cggatgccct
      121 gatcgagttc tacgcccagg ccatcgtgga cggcaccgat ccggggcatc cgtcggcctg
      181 gccgcgcggg gtgacctgcc gcaccccgct gaccgggatc accaactcca tcgtcgaggg
      241 cgccaacctg gcgttcgggc tgtacaccgc cggcgaccgg ttctggtcgc ggctcgatga
      301 tcgccagcag ggtcaggtgc tggcctggct gcggcaccac gcgctgcgcg aggtgtggca
      361 gaacaactgg cagctcttcc ccgcgatggc cgaggcgttc atccggcacc gcggcggcga
      421 cgcccgcgga ctgcacggcg agcgcaacgt cgcccgggtc gagggctggt acctcggtga
      481 cggctggtac accgatggtc cgtcccacgc ggtggactac tacaacgcgt gggccatcca
      541 cccctacctg tgggcctggt accggatgac cgaccgcacg cagacgtacg agggacagcg
      601 ccacctggag cggctcgcgc agttcgtcga gcggatgccg tcgttcgtcg cctccgacgg
      661 ggcgctggtg cacttcgggc gttcgctcac ctatcgcacg gccgcgctcg cgccgctgtg
      721 gtgcgcggag atctcgggcg tcagccccct gccgccggcg cgcaccaggc aggtcgcgac
      781 ctcggtgctc gacggattcg tgcggcacgg cgtcggcgcc gatggtcagc tgcacctggg
      841 ctggtacgac gagcacctgc ccgcgtcact gcaggactac agcgggttcg ggtcgccgta
      901 cctggccggg atcggcttcc tcgggctggc gctgggcgcc gggcatcggg tgtggacgga
      961 tgcggcagag ccggcggagc atccggcctc cacctgcccc ctcgacggca cggggatcac
     1021 gctcagcacc gcgggtggcg acgcgccggt gatcctcgtc aaccacggct ccgaccacgc
     1081 cgagctgccc gtccaggacc acggcatccg cgaccccgac gacccgcact acggctcgtt
     1141 cgcctactcc acgcacaccg cacccgaggt gggcgacggc tggctcgagg ccgtcgacgg
     1201 gcacgtgtgc ctgttcgacg accagggccg cccgagtcgc cgcgccaagc tgcgcgggcg
     1261 gatgcaggac ggtccgctca gcgcgtcggt gcactacccg cagatcgacg gaaggctgct
     1321 ggtcggggcg gcgatcgtca cggcatccgt ggtgaatcgc ggtacggagg tgcgcgcgca
     1381 cctggtgatc gcgccgaagg tgcacgaccg catccgtgag ggcgccttcg cgctcgccgc
     1441 ggacgaaccg cccgagcagg acgtggcctc cgatcgcctg cacgcatcgg tcgccgacgg
     1501 cacgcatcgc gtgatgctga ccggcctgca cggatgggac gacgcaggcg tcgtgacgca
     1561 ccgcggcacg aacgcgatgg gcgagcactc cgcgatcccg atcctcaccg ccacacgcgg
     1621 cgaggatgag accgtgcatg tcgcggtcca ccagctgctg cgcgaccggg acgatccgga
     1681 tgccgcactc gccggtctgc gcgtggctgt ggacggcacc caggtgcgcg tggagtggcc
     1741 ggacgacagc gtcgacgtcg agttcgacct gcgcaccgtc gtggagtggg acggtcagcc
     1801 gggcaggccg ggcggccgga ggaactgaag gggcgcggct ccgcgttcgt ctgcgctcgc
     1861 cgcgtgaatc tgcgcacgcc tgtgcgcttc gcgcggagaa gggcgcttcg cgcggaggag
     1921 cgcgcttcgc gcggacgaat ccctggaaac tctccgcgcg aagcgcacga atccgcgtca
     1981 ggcgctcgcc gctgacgcgg tcggatgcgt cacatccagg cgtcgatgcg gtccccggcg
     2041 gaggcggatg cctcgcccag cgccagctcg atcacgaagg cgtgctccgc ctcgagatcc
     2101 gcggggatgc tcacgtcgca gtacagggcg tcgtccaggt cgaagccgat gtcggtcacg
     2161 cgcaccggga ggtcgcgtcc ggagtcgagc aggcgcgccg agaccggcat ccgctccgcg
     2221 ccggccggga accgcatccg caggatgccc gtgacaggcc ggttcacgac cgtgacgaac
     2281 agccggtcgc cgtctccggt cagccatccg gcgcccgtgc ggtccaggtc gacgtgatgg
     2341 gcgccgtaca ccgcatccgc gttcgcggcg acccacgcgc ccagctgctc ggcgatctcg
     2401 tcctcgtgcg aggtcatgcg gccgaggccg tcggggccga agttcacgac aagattgccg
     2461 ttcatcgagc ggcagcgcat cagcaggtcg atgaggtcgt ccgcggtctt ctggtactga
     2521 tcacgggtgt cccgcagcca tccccaggcg ttcgggccga tcgttatcac gcagtcccag
     2581 tcgttcccgc cgagcatctc gaagctcgcc ggcagtttgc gctcccagcc ctgctcgtag
     2641 tcgcccagca ggtcgccgtt ggaatcgaag tgccgggcgc cgtgctcgtc gttgcggaag
     2701 cgagagccga tgatcaggcc ggggcggcgt gcgcgcagct ccgcctcgag cgcgtgggtg
     2761 aactcgtgcg aggcgatcca cgacgcatcc caggtgccgt cgaaccacag gccgcgcgcg
     2821 gcgggatagc ggtccagcag ctcgagcagc tgggcgcgcg tgtactgcag gaagtcctgc
     2881 cacccggcgc gctccgcatc ggtcgcgggc accgtgcgga gataccccgg gtggtgccag
     2941 tcgagcaccg agaagtacag gaacacgtcg atgccctcgg ccgtgtacgc gtcgaccacc
     3001 tcgccgacca ggtcacgccc gcacggcgat tcgcgcaccg tgtaatcgga gtgcgcagag
     3061 ggccacagcg cgaacccgtc gtgatgcttg gtggtgaaga tgacgtagcg cgcgcccatc
     3121 ctcttcgcct gtcgggccca ggcgtgcgca tccagttcgg ccggatcgaa gctctcgttg
     3181 agtcggtcgt agatcccctg ccagccctcc ggggctgtgg gcccgctcca tgcgcggatc
     3241 cactccggcg ccgccgatcc gccggccgcg ctcacgccct ggtactcgcc gccgaggatc
     3301 gagtacaacc cgaagtggat gaactggccg agcccgtacc ggcggaagcg ctgcatggcc
     3361 tcgtcctcac ggtgcgagac ctgcgtgccg aatcgcagcg ggatcggctg gcggtcggag
     3421 gggagtgcgc gttcggtcca gggagaggtc gtcatcgggt cctcgttcgg cgttggtaag
     3481 cggtttccca gtctatcggc tgcgcccgcc gaacggggcg cccggcagcg cggaatccgc
     3541 cggcaggccg agcgccttca gcacgtgcgg aagcacatcg gcgtggtcca cgacgggttc
     3601 gacggagggg acgtccggac ccgccgcagc catccacgcc agccgctcct cgtcgctgtc
     3661 gccaccgtga tgccccgcgt cgaggtgccc gtgatcggtg agcacgatga cggtccagtc
     3721 ctcatgctcg cggttctcgc gtgcatcgat cgctgcgagc agcacgccga gctgctcatc
     3781 gcacgtctcc acggcccggc gatagcggtc ggtcacgcct tcggtgtggc cgacgatgtc
     3841 cggcagcacg gcataggtga acaccgccgc gtgagcgcga tggagcagtt cgcgggcggt
     3901 acgcgacatc acggcctcgt ccatcagggc gacgacctcg agcggcccgt cctcagcgcc
     3961 ggccggcagc tccggccggt acccgccgcc ggcgaagagc
//
LOCUS       DATMSX010000274         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_61208,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000274 DATMSX010000000
VERSION     DATMSX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61208"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VN041_08290"
     CDS             <1..225
                     /locus_tag="VN041_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWU29076.1"
                     /translation="ARLLEGLRSPLPRLTDRETEVLRLLATGAANREIARTLFVTEAT
                     VKSHLAHIFTKLDVDSRSRAIHVAREAGLI"
     gene            244..690
                     /locus_tag="VN041_08295"
     CDS             244..690
                     /locus_tag="VN041_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HWU29077.1"
                     /translation="MKRLVNAAFAYMLAGVASGLFYREFTKANDFTDATFTQLSVVHT
                     HLLILGFVMLLIVLVLEKVFAISRSPRLFSWFLWLYNAGVIVTAGMMTWHGILTVLGQ
                     ESSAMIAGIAGLGHILITAGLIVFFIALRRAVASDAGAAGRAALTG"
     gene            932..1096
                     /locus_tag="VN041_08300"
     CDS             932..1096
                     /locus_tag="VN041_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29078.1"
                     /translation="MCRAVTCRTCGKTTWAGCGQHVDSVMAGVPAARRCRGHEDEPRT
                     GFLAKLFGRS"
     gene            1101..>1877
                     /locus_tag="VN041_08305"
     CDS             1101..>1877
                     /locus_tag="VN041_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU29079.1"
                     /translation="MRVIIIGGVAGGMSAATRLRRLDETAEIIVIEQGDHVSFANCGL
                     PYYVGGVIADRDALLLQTPASLRARFELDVRTGHRAVAIDRDARTVSIQDLSTGQVTD
                     EHYDHVVLATGAVPRDALASVEGGPVVSTLRTIDDVDRITLALADLPEAGRAVVAGGG
                     FIGLEAVENLVHRGLAVTLVQHGGHPLSPLDPEMASLVVDELTARGVDLRMHSSVELL
                     DADGVHLDDGTVVPADIVIDARGVRPAASLAVDAGLALGPT"
BASE COUNT          276 a          683 c          625 g          293 t
ORIGIN      
        1 gcgcgcctgc tcgagggcct gcgcagcccg ctgccgcggc tcaccgaccg tgagaccgag
       61 gtgctgcggc tgctcgccac cggagccgcg aaccgcgaga tcgcccgcac cctgttcgtc
      121 accgaggcga ccgtcaagag ccacctcgcc cacatcttca ccaagctcga cgtcgacagc
      181 cggtcgcgcg cgatccacgt cgcccgcgaa gccgggctga tctgacccat tggagaaacc
      241 atcatgaaga gactcgtcaa cgccgcattc gcgtacatgc tcgccggggt cgcatccggc
      301 ctgttctacc gcgagttcac caaggccaac gacttcaccg acgcgacgtt cacccagctg
      361 tcggtggtgc acacgcacct gctgatcctc ggcttcgtga tgctgctgat cgtgctggtg
      421 ctcgagaagg tcttcgcgat ctcccgcagc ccgcgcctgt tctcctggtt cctctggctg
      481 tacaacgccg gggtgatcgt gaccgcggga atgatgacct ggcacgggat cctcacggtg
      541 ctcggtcagg agtccagcgc gatgatcgcc gggatcgccg gcctcgggca catcctcatc
      601 accgccggtc tgatcgtgtt cttcatcgcg ctgcgccgtg ccgtggcctc ggatgccggg
      661 gcggccggac gcgccgcgct caccggctga tccggagggc cgaccgcacg ggcgcgtgac
      721 ccgcacgcgg ctccaggtcg cagcggatgc cgcccattcc ggcgcacccg atctcaggtc
      781 gcaatggatg ccgcccttct gccgtctgaa cggcatccgt tgcgacctgg gccatgggtg
      841 agcgctgagc agtcacgctc agcgagttcc aagatacccc cgggggtatt tctgatacgg
      901 tggcactcga cgacagagag gacccgaatc gatgtgtcga gccgtcacct gccgcacctg
      961 cggcaagacc acctgggccg gatgcggcca gcacgtggac agcgtcatgg ccggagtgcc
     1021 ggcagcgcgg cgctgccgcg gtcacgagga cgagccgcgc acgggcttcc tggcgaagct
     1081 gttcggaagg tcctgagcgc atgcgggtga tcatcatcgg gggcgtcgcc ggcggcatgt
     1141 ccgcagcgac ccgcctgcgc cgcctggacg agacggccga gatcatcgtg atcgagcagg
     1201 gcgaccacgt cagcttcgcc aactgcggcc tgccctatta cgtgggcggc gtgatcgccg
     1261 accgcgacgc cctgctcctg cagacgcccg cgtcgctgcg tgcgcgcttc gagctcgacg
     1321 tgcgcaccgg ccaccgcgcg gtggccatcg accgggatgc caggaccgtc agcatccagg
     1381 acctgtcgac cggacaggtg acggacgagc actacgacca cgtcgtgctc gccaccggtg
     1441 ccgtgccgcg cgacgcgctc gcatcggtcg agggcgggcc ggtcgtgtcc acgctgcgca
     1501 cgatcgacga tgtcgaccgc atcacgctgg ctctggccga cctgccggag gccggccgcg
     1561 cggtcgtcgc aggcggcggg ttcatcgggc tcgaagccgt cgagaacctc gtgcaccggg
     1621 gcctcgccgt cacgctcgtg cagcacggcg ggcatccgct cagtcctctc gacccggaga
     1681 tggcgtcgct cgtcgtggac gagctgaccg cccgcggcgt ggatctgcgc atgcacagct
     1741 ccgtcgagct actggatgcc gacggcgtgc acctcgacga cggcacggtc gtccccgccg
     1801 acatcgtcat cgatgccagg ggcgtccgtc ccgcggcatc cctcgccgtc gacgccggcc
     1861 tggcgctcgg gcccacc
//
LOCUS       DATMSX010000275        19106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_184069,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000275 DATMSX010000000
VERSION     DATMSX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184069"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VN041_08310"
     CDS             complement(<1..283)
                     /locus_tag="VN041_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29080.1"
                     /translation="MTSRAPGPSVRLGVLVPSSNSNAESSIQRMLAGQEDVAAHVSRF
                     RLPASLGDRIDAEVLGEAPALLADVEPDAVAFHGTSGSWTGFDGDRLLCE"
     gene            complement(304..921)
                     /locus_tag="VN041_08315"
     CDS             complement(304..921)
                     /locus_tag="VN041_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU29081.1"
                     /translation="MGTTSTPKSIPEEGLDRDFWISHLGAQLREQRSGRFTVEELADR
                     AGVSAGLISQIERGIGNPSFATLLRLANSLDLPLASMFADPDPGNEQMLVRRADRRRI
                     EIPSQGIMMELIVPDAERKLGVVNMTIPARFDGSSVPHSHEGEEVVLINAGRLVATVA
                     GRDFELEAGDSLSYDASLPHWWSNRTDSAAVMLAISTPPSLGRAH"
     gene            complement(932..1681)
                     /locus_tag="VN041_08320"
     CDS             complement(932..1681)
                     /locus_tag="VN041_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWU29082.1"
                     /translation="MHAPLRDEVGLITADPTPTLLGLLEVAGIDSIVIDAEQTGITVG
                     ACADAVQRLRGSGVRVGIRVPALDDDCLLAFANTGADELVLPRVRRVEELENAHRATR
                     FPPEGERPRQVSPASRYGTAYAHVPVLSVLVETTDAVDAIEQFAAHPLFEGAWIGPTD
                     LADDLARADRAGELDSAVQRVIDVVAASGHPIGLPAPSAARAAEVHARGANRAAVYWE
                     REVTATLRTLAASRGAEDALSSRYNEPSSTK"
     gene            complement(1684..2094)
                     /locus_tag="VN041_08325"
     CDS             complement(1684..2094)
                     /locus_tag="VN041_08325"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU29083.1"
                     /translation="MGRPPADISRLVHNAHDIGYSDLAKIGLKGQRDQGQVCNLISRD
                     LSGSDDLNVSLGRILPGQHHLCHHHPDVSEFYVIISGTPIVHLDGTDYRARPGDGIYI
                     PRGTSHGITNDTDENIEMVVGMSKPADWQFVCDE"
     gene            complement(2096..3013)
                     /locus_tag="VN041_08330"
     CDS             complement(2096..3013)
                     /locus_tag="VN041_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWU29084.1"
                     /translation="MRDLIGYGEHPPVVGWPNGARVAVSLVLNYEEGAELSIEAGDER
                     DEDISIFGGWSADPSRRSLMKESFFEYGSRVGIWRYLGMFREFGVRSTVMACGVALER
                     NPEAARAIVRDGHEICGHGYKWRGLVGMTAEEQRAEIRRCVASIEETAGVRPTGWYVR
                     EGITEETRDVLAEEGFLYDSNSYSDDLPYYVDTEQGPHLVVPYSGDTNDARFWGSGSL
                     GSPDDYFAVLRDTLDCLLIEGAETPRMMSVGVHLRIGGRPSVAMAVRRFLAYAQEQQG
                     VWFATREEIARWWLEHAPAGSSRELEEAR"
     gene            complement(3095..3913)
                     /locus_tag="VN041_08335"
     CDS             complement(3095..3913)
                     /locus_tag="VN041_08335"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU29085.1"
                     /translation="MTAVFESPRRIADGVYWLGGCLSAFGQGEEVHYHVNAYLVIGEE
                     RTALIDTGDPAHRAVVLAQLDEALAGRTLDYLVPTHPEIPHAGNLPALMERFPQATVL
                     GEVRDYHLHFPEFADRLRAVPAGQSVDLGGRELVLMPAHIRDLENTTWAWDSGAGVLF
                     VSDGFSFIHDVPSPDEEADEPAHLPGQCRLFSGEMPEPPSVAQAAYGTGRALYWTKFV
                     DVSDAFTAIEGLLDEHPTGFIGPAHGNVIDDVDGMLRTSLAAHRAVYQSRGLGQ"
     gene            complement(3910..4614)
                     /locus_tag="VN041_08340"
     CDS             complement(3910..4614)
                     /locus_tag="VN041_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29086.1"
                     /translation="MSATTVHRLGGDIDLDERVSWCPPGVRASQPVSCYLIIGETGAL
                     LVDTGIRTHEQEVLAALQELYDGGVPLAVLLTRTEMECCLNLPAIESRFPIDAVWYTG
                     GITVPRSHAEVRRVSVEPGTSQWIEPHPGMRIELISPMMRLLPTLWVHEPETGALLTS
                     DAFTHGDGPAENGLAKFRWFTRAETGDIAAHVRSVVAERRISLIGPGYGRPFEGEEVA
                     EQTAALAAAIERIGLS"
     gene            complement(4628..5299)
                     /locus_tag="VN041_08345"
     CDS             complement(4628..5299)
                     /locus_tag="VN041_08345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWU29087.1"
                     /translation="MIEICGKQVRDTFAELLDPATTALVVIDMQKGAVSKGGAIADSG
                     HDMSMMPRIVDQCGRAIRAARANGVPIFHICVENLPDGKSSTAAWLRALYTVGQGRST
                     DLGRLSAKGDPLTEFAEACEPEGDEIVIRKRRQSAFVGTELALLLRSQGIESVAIVGV
                     STGGCVEATLRDAGSNDFYAVLIDDAVGAYDETVHEAALTVMRARHDHCSLDEAIAVW
                     ESAAR"
     gene            complement(5370..6971)
                     /locus_tag="VN041_08350"
     CDS             complement(5370..6971)
                     /locus_tag="VN041_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU29088.1"
                     /translation="MKHSRLALLGAVAAAVSLVVTGCSGTPSSDGTAPAASKPESVVV
                     AVGAVPDSLTPSPWGGSASHVVLSGLGSQLMAYKGAGSEESCLKPSTDLTGRLAESAE
                     LSPDGTGVIVKLRDLTSQWGNKLSAEDVAWSLQIGMVRQPVMKGTLTTSGFDVDNLTK
                     IIDEHTIQLNTKVVTSYTLASLQNNLFYIHDSVEAKKHATADDPTANDWLSKNLADYS
                     GWDLEEFTPGTSLTLKADSHWEGKRGSVKRVVIKAVSNTATRSQLVESGEAQVAGSFE
                     YDQYASLAKAPGVQVLDCVGQTRDTLMINTKTGPLADPAVRRAVSQAIDRAALVKGAY
                     SGYGEPSKAIFPGVKATPSYTFDKAAAKKALADAGYPDGFDMELSYSVTRPGPVAERS
                     AVLIQSMLKDVGIRVALKNIASTTDFSTAVIDGRYQAILYSEPIVINDPAFYSYAFYA
                     GGAPSNSTGWADPRFDEARLKMADVKTSEADRKTLLQDAADYIDEGSPILALVQTKNM
                     LARQKGLTGANPLTNNQIYFAALGG"
     gene            complement(7033..7851)
                     /locus_tag="VN041_08355"
     CDS             complement(7033..7851)
                     /locus_tag="VN041_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU29089.1"
                     /translation="MENIITETMAAEFPREIRPGVFWMGACLPFHLADRVIHGHNSAY
                     VVKGTEASMIVDTGNPSSWPTISAKLDELLGDEPLTYVFPTHPELPHTGNLPRLVEKY
                     PDIKVVGDMHDYHLFYPQIVPNLNSRVPGDVIDLGGGQEVRIVEAMIRDLPNTQWAVA
                     SREKVMFTADGFCYMHRPELDDEDPLHLPGECALTTDELSQPIAVENAAFFTGSALYW
                     ARFNNDADRVYDRVLALVDELGVEIVAPTHGNVITNVRDVEPIIRAGHKQAYRY"
     gene            complement(7863..8690)
                     /locus_tag="VN041_08360"
     CDS             complement(7863..8690)
                     /locus_tag="VN041_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29090.1"
                     /translation="MFTKVHDGGLTVLGGAYELSDLVSWVPRGSTGYQASNCYLLSEG
                     EDHLLIDSGLAVHSEQVLDDLAELIGEGGGVSMFFTRPEMDCVSNLEPIARRFDILRL
                     FTGGVINPFDAFDDVSRMALRGRRQQIDGTRTVEGDALERSMTIEVAPGRILDVQSPL
                     LRLLPTFWGWDEKTGALFTSDTFTHGVMDRPDGYRFIDDTVKDDTTPAQVAAHLFAKY
                     EWLPRATKHPLRDWLVETFDRFDPEVIAPSRGAVIRGRASVRRHLDLMLAALADDAA"
     gene            complement(8763..9824)
                     /locus_tag="VN041_08365"
     CDS             complement(8763..9824)
                     /locus_tag="VN041_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29091.1"
                     /translation="MIRYIISRLIIAVLLLWGLITLSFGLVSLLPGDPAKALLGEFAT
                     PQDVARINEQLGLDKPFLERYLDYLVRVLHGDLGTSFFTGSSVSEELWTRLPNTLVYL
                     VPGLILALIIGLAMGAVAAYRVGRLPDRTFGVVVSILMAMPEFVLALILLFIFYQQLK
                     VAPAPLGMLSALDSPPPKVTGWVPVDAVIAGQWGTVGSIFSRAVLPTVTIGLFFAAAF
                     GKTVRTGMLQILGSPQIEFARACGLRPAQVFRYALTDVRGALMTYMVLLFAASLSGAA
                     IIESVFSWPGIGGWSLDGVLKGDVPVIQGFVLVMGATSLLGYVVLDTLITLLDPRTRQ
                     NVAPRRREPAAQPAVATAV"
     gene            complement(9821..10645)
                     /locus_tag="VN041_08370"
     CDS             complement(9821..10645)
                     /locus_tag="VN041_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HWU29092.1"
                     /translation="MALIELTDVTKTFALPHGGAKVAVDGVSLSVEHGECLGIIGESG
                     SGKSTLGRLILRLYDADSGSVVLEGQDIRALSRRELRRHRHRWQIVFQEPYASLNPRL
                     TIGQIVEEPLIVHGGYGGRAARRERVTSTLQEVGLSPDFLDRRPANLSGGQQQRVGIA
                     RALVTDPSLVVLDEPTASLDLTIRASTLRMLNRLRESRGLTYVFISHDIETVRHFCSR
                     VVVMHHGRFVEGGTAQQVLDAPVEDYTRNLMAAAMPPIPYRGRTAVLDDLDVSGES"
     gene            complement(10647..11498)
                     /locus_tag="VN041_08375"
     CDS             complement(10647..11498)
                     /locus_tag="VN041_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29093.1"
                     /translation="MSGLEVTGLVTDFENRTTSIRALHGVSFGVGESEVVGIVGESGS
                     GKSTVVRSIIKLLMPPGKVTEGHVDFYGASLLDLKERELRRIRGKEIGFVAQNPFSAL
                     NPVLRIEKQFDNIAKAHGAPRGQELHRRAVDLLEATGVKDAERVIRGYAHELSGGMAQ
                     RVVIAMALFLNPRLVIADEPTTALDLTVQRQVLDTLTRLTVDSGRSMLIVTHDLGVVA
                     NYCDRVLVMYQGRIVEQGPVSRVFVTPEHEYTASLLDSVVRPEESPAGGTRNIATVGV
                     RPKVMEY"
     gene            complement(11495..12382)
                     /locus_tag="VN041_08380"
     CDS             complement(11495..12382)
                     /locus_tag="VN041_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29094.1"
                     /translation="MSVQSAPPTSASIRVVRWSRRGRLRLGARWNGIAATIAVIVIIG
                     LPVLALIGPYPIDPFRPDPNAIGIAPNGVHWFGTDGNGMDVFARTIEAAKLDVPISVA
                     ATLLALVVGVPIGLFATTGRVGDAVMRVVDAFAALPIIVIAIVAIQLMGGGAWNVILA
                     IAIVGAPRFVRLSRASAIALRSSRYVEAAVAIGCSPLRVAFNHVFRNAYGVVLVQATL
                     TAANALGTIAALNFLGVGVNPPVPTWGAMISDGASMLIRGEWWAAAFPTLAMLLVIAS
                     FNVIAGAIENRIERVERAR"
     gene            12684..13628
                     /locus_tag="VN041_08385"
     CDS             12684..13628
                     /locus_tag="VN041_08385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWU29095.1"
                     /translation="MSPTPSPVRYLLHDGELIDYADAQLHVLSTSTKYGVGVFEGFRA
                     YWSEEDGELYAFRVEDHMRRLIDSMRVVEIDGPTDTGVLADQLIGLIRANELRSNLHM
                     RTQVFVDSPDGKPDDTGPATVFMAAIPMGNYFGATGLDVQISSWARLSDRAMPPRVKS
                     IANYQNGRLALLEAKRNGYGAALLLTESGHVAEGAGYNVFMVRQGRLCTPPSTDSILE
                     GITRDSVLQLATTQLGFDVDIRPIDRTELYSADEIFVCGSAAEVNHVTSVDRTKIGDG
                     STAGPITASLQGLYADAVRGRIADFGHWVTPVYGTASA"
     gene            13625..14527
                     /locus_tag="VN041_08390"
     CDS             13625..14527
                     /locus_tag="VN041_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWU29096.1"
                     /translation="MTGTTAHADFLHARGEFDPGAEPVLSVVQGQRFRLEARSVLTDP
                     RFETADDFSIFSIPVTGPVRIEGVRPGDLVRIDVHDIRIADRGGMVTMPGRGGFGEPL
                     KMHGMVVPVHDGFAHFPDDVRVPIRPMIGKIGFAPEGESPNSSTVGRYGGNMDCRDII
                     TGSSLFITAQVDGGLLYAGDLHAVQGDGECSLTAVEMAGSVELSCCVVHPAPVRGPVV
                     FAEDRMIVLGDGEDLDEAATVALDEMMTIMRADRGWTRERTAMLLSAAADVAVSQLVN
                     ARRSVKVSLDTRYLLTAPFQTKED"
     gene            14529..15953
                     /locus_tag="VN041_08395"
     CDS             14529..15953
                     /locus_tag="VN041_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU29097.1"
                     /translation="MYDLLITGGLVVTPSGAEPLDIAIADGRIAALAFAGTTPLDSAA
                     RVIDVGGKLVVPGGVDPHVHTNSVLPTAAESGIMCFGADRVSEGAIYGGTTTLVDFAH
                     WKPGDDLSDSFARKAAEWNGQTYTDFALHGTFKEPEVPFSVLEQIPDAVRNGHGSWKV
                     WMTNTTPTRPHQKTDLGNMWGIMEQTAAAGAMLAVHAEDDDIVMYSYRRLKHQGAIGI
                     ENMHHAHNVLSEQLSFQRAITLARHVGAAIYLMHVSANVGIDAIRKARGEGAPIYGEI
                     LPHYAYFSAENYREPHGAIYHTYPSLKDADDRDHMWPALIDGTLSTLATDGVCTDFDV
                     KTRGNTIYDATGGHAGVEMRMAVAYTEGVVGRGLDLTRYVDLTSANAAKILGLYPRKG
                     AIAVGSDADLAVIDTDVDRRVTAADLHEADYTPWEGYRTRAWNMMTLLRGQVMVDDRE
                     LVAGPVGELQSQRVSSDVLSRPAC"
     gene            15964..17454
                     /locus_tag="VN041_08400"
     CDS             15964..17454
                     /locus_tag="VN041_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29098.1"
                     /translation="MTAPAAAPVRMLVISLMASMLVVSFNQTVLNTALPTIIAELGGL
                     DRIGWIVAAFVLTSTLSMPLAGALSDVLDRKRLLLGASIVFGLGTLLGALSWNLDVLI
                     IARLAQGIGGGGVMVLTQTIMAAAVPSRERAKYAGAFGSVWAVATISGVLIGGWLTDG
                     PGWRWVFWGTLPLLVVAMVLGARFIAPSRPSDARVQPDIAGMLLLCIATTALVTATSA
                     SLPAEWSWFIGAVGAGAVCALVFAERRAEQPVLPGILFRSRTFVLATLSGMLCSGVAS
                     FVVLSYLPSFAQMVLGLSALQAGLLLLPMIVATVTSSTLTGFAVSRTGRYILIPALGA
                     GAVVVGLLLLGTLGPDTTPLRLSLSAALLGAGIGTNVLLLTLLAQNALPARNIGVATA
                     ANNFFRQAGATMGISLVGVAFASRLHDALASALDTRGVPQREDLPLATISPEQLALLD
                     PGLRSAVAEAYASALPPLFLALIPLALASLVMILALERRPLAESHI"
     gene            17557..18372
                     /locus_tag="VN041_08405"
     CDS             17557..18372
                     /locus_tag="VN041_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HWU29099.1"
                     /translation="MCRLFAFVSPVTASARGELGADGMESLLSLARLHGDGWGRASVR
                     APGAAPIVTRSAISAAEDPDFDAALATPCRAAVVHLRWATAGLPVNIRNAHPFEADGI
                     AFAHNGTIKPRETLQGLLSAGSVAALQGTTDSEMYFALIRERVSAGEPLPEAAAHVAH
                     TLRELFPLASLNALIIDAEHLVVVHASATSILTEHDLARLAPVAELLPDEHNEDYFAL
                     RIRETPDGTVMVGSTGVAGLDWEPLPAESVIAIRLDDRRRSTIGIASLLAARS"
     gene            18537..>19106
                     /locus_tag="VN041_08410"
     CDS             18537..>19106
                     /locus_tag="VN041_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWU29100.1"
                     /translation="MPTVSAHVALTLAQHIDHVFGVMGNGNAYFLDAIETQTDATFTA
                     VRHEQGSVVAADAHFRASGRIAAATTTYGAGFTNTITSLAEAVQAHVPLVLVVGDEPT
                     SGPRPWDVDQIALASAVGARTYTTGRADAAATTVIAIEHALTYRVPVVLAIPYDVAAL
                     EAGPVPEAPAPRIPAPLAPRGDLVKHPAGE"
BASE COUNT         2959 a         6594 c         6565 g         2988 t
ORIGIN      
        1 gctcgcacag gagtcggtcg ccgtcgaacc ccgtccacga tcccgacgtg ccgtggaagg
       61 cgacggcatc cggctcgacg tctgcgagca gcgccggcgc ctcgcccagc acctccgcgt
      121 cgatccggtc gcccagcgac gcggggagcc ggaaccggct gacgtgcgcg gcgacgtctt
      181 cctggcccgc cagcatccgc tggatcgagg actcggcgtt cgagttcgaa gacggcacga
      241 gcacgccgag gcgcaccgag ggcccggggg ctcgggatgt catggttcct ccgagagtcg
      301 cgctcagtgc gcgcggccga gcgaaggcgg cgtcgagatc gcgagcatca cggctgccga
      361 gtcggtcctg ttgctccacc agtgcggcag ggaggcgtcg tagctgaggc tgtcgcccgc
      421 ctcgagctcg aagtcccgtc ccgcgaccgt ggcgacgagg cgaccggcgt tgatgagcac
      481 gacctcctcg ccctcatggg agtgcggaac cgatgagccg tcgaagcgcg cggggatggt
      541 catgttcacg acgccgagct tgcgctccgc atccgggacg atcagctcca tcatgatccc
      601 ctgcgacggg atctcgatgc ggcgacggtc ggcacggcgc acgagcatct gctcgttgcc
      661 cgggtcaggg tcggcgaaca tgctggccag cggcaggtcc agcgagttgg ccaggcgcag
      721 cagcgtggcg aaggacggat tgccgatgcc gcgctcgatc tggctgatca gcccggcact
      781 gactcccgcc cgatcggcga gctcctcgac cgtgaagcgg ccggagcgct gctcgcgcag
      841 ctgagcgccg agatgcgaga tccagaagtc ccgatcgaga ccttcctcag ggatcgactt
      901 cggcgtagag gtggtaccca cgttcagccc ttcacttcgt tgacgatggc tcgttgtatc
      961 gagacgatag cgcgtcctcg gcgccccgag atgcagcgag ggtccgaaga gtcgcggtca
     1021 cctcgcgctc ccagtacacc gcggcccggt tcgcaccccg cgcgtgcacc tcggcggcgc
     1081 gggccgcgga gggagcggga aggccgatcg gatgccctga cgcggccacc acgtcgatga
     1141 cgcgctgcac ggcggagtcc agctcccccg cgcgatccgc ccgcgcgagg tcgtccgcga
     1201 ggtctgtcgg gccgatccac gccccctcga agagcggatg cgcggcgaac tgctcgatgg
     1261 cgtccacggc atccgtcgtc tcgacgagca cggacagcac gggcacgtgc gcataggcgg
     1321 tgccgtagcg cgaagcgggc gacacctgcc gcggccgctc cccctcagga gggaaccggg
     1381 tcgcacgatg ggcgttctcg agctcttcga cacggcgcac gcgcgggagc accagctcat
     1441 cggcgccggt gttggcgaag gccagcaggc agtcgtcgtc cagggcggga acacggatgc
     1501 ccacgcgcac cccgctgccg cggagtcgct gcacggcgtc ggcgcaggcg ccgacggtga
     1561 tgccggtctg ctcggcgtcg atgacgatcg aatcgatccc ggcgacctcg agcaggccga
     1621 gcagggtcgg cgtggggtcg gcggtgatca gcccgacctc gtcgcggagc ggtgcgtgca
     1681 cgttcattcg tcgcacacga actgccagtc ggcgggtttg ctcatcccga ccaccatctc
     1741 gatgttctcg tcggtgtcgt tggtgatgcc gtggctcgtg ccgcgcggga tgtagatccc
     1801 atcgccgggg cgcgcccggt agtcggtgcc gtcgaggtgc acgatcggcg tgcccgagat
     1861 gatcacgtag aactcggaca cgtccgggtg atggtgacac aggtggtgct gcccgggcag
     1921 gatccgcccg aggctcacgt tgaggtcgtc cgagccgctc aggtcgcggg agatcaggtt
     1981 gcagacctgc ccctggtcgc gctggccctt caggccgatc ttggcgaggt cgctgtaacc
     2041 gatgtcgtgc gcgttgtgca cgagacggga gatgtccgcc ggcggacggc ccatgtcagc
     2101 gcgcctcctc aagctcgcgc gagcttccgg cgggagcgtg ctccagccac cagcgcgcga
     2161 tctcctcacg cgtcgcgaac cagaccccct gctgctcctg cgcgtaggcg aggaaacggc
     2221 gcacggccat cgcgacactg gggcggcctc cgatgcgcag gtgcacgccg accgacatca
     2281 tccggggcgt ctccgccccc tcgatgagca ggcagtcgag ggtgtcgcgc agcacggcga
     2341 agtagtcgtc ggggctgccg aggctgccgc tcccccagaa gcgggcgtcg ttcgtgtcgc
     2401 cggagtaggg cacgaccaga tgcggaccct gctccgtgtc cacgtaatag ggcaggtcgt
     2461 cggagtagga gttggagtcg tagaggaacc cctcctccgc caggacgtca cgggtctcct
     2521 cggtgatgcc ctcccggacg taccatccgg tgggccgcac gccggcggtc tcctcgatgg
     2581 aggcgacgca gcggcggatc tcggctctct gctcctcggc cgtcatgccg accagcccgc
     2641 gccacttgta cccgtgcccg cagatctcgt gcccgtcgcg cacgatcgca cgcgccgcct
     2701 cggggttgcg ctccagcgcg accccgcagg ccatgacggt ggagcgaacc ccgaactcgc
     2761 ggaacatgcc gaggtagcgc cagatgccca cccggctgcc gtactcgaag aacgactcct
     2821 tcatcaggct gcgacggctg gggtcggcgg accagccgcc gaagatcgag atgtcctcgt
     2881 cgcgctcgtc gccggcctcg atcgacagct cggcgccctc ctcgtagttc agcaccagcg
     2941 agaccgccac acgtgcgccg ttcggccagc ccacgacggg cgggtgctct ccgtatccga
     3001 tgagatcacg catggtcggt gcttcttcct ccgagaccgg gcggcagcat ccttgcgttc
     3061 actccggtag tcctatcttc attatagtga attctcactg gcccagcccc ctgctctgat
     3121 acacggctcg gtgcgccgcg agagaggtgc gcagcattcc gtcgacatcg tcgatgacgt
     3181 tgccgtgcgc gggtccgatg aagccggtcg gatgctcgtc gagcaggccc tcgatcgccg
     3241 tgaaggcgtc gctgacgtcg acgaacttcg tccagtagag cgctcgaccc gtgccgtacg
     3301 ccgcctgcgc cacagacggg ggctcgggca tctcaccgga gaagagacgg cactgacccg
     3361 ggagatgcgc cggctcgtcg gcctcctcat ccggcgacgg gacgtcgtgg atgaacgaga
     3421 agccgtcgga cacgaagagg accccggccc cggagtccca cgcccaggtg gtgttctcca
     3481 gatcgcggat gtgcgccggc atcagcacca gctcccgtcc gccgagatcc acggactgcc
     3541 cggcggggac cgcgcgcagg cgatcggcga actccgggaa gtgcagatga tagtcgcgca
     3601 cctcaccgag gacggtcgcc tgcgggaacc gctccatcag agcgggcagg ttccccgcgt
     3661 gcgggatctc ggggtgggtg gggacgaggt agtcgagggt ccgcccagcg agagcctcgt
     3721 cgagctgagc caggaccacc gcgcgatgcg cggggtcgcc ggtgtcgatc agcgcggtgc
     3781 gctcctcgcc gatcaccaga taggcgttga cgtggtagtg cacctcctcg ccctggccga
     3841 aggcggacag gcatccgccc agccagtaca cgccgtcggc gatccggcgg ggcgactcga
     3901 acacggcggt catgacagtc cgatcctctc gatggcggcg gcgagcgcgg cggtctgctc
     3961 cgccacctcc tcgccctcga acggccgacc gtacccggga ccgatcagcg agatccgtct
     4021 ctccgcgacg accgaccgca cgtgcgccgc gatgtccccg gtctccgcgc gggtgaacca
     4081 gcggaacttc gccaggccgt tctcggccgg cccgtcaccg tgggtgaagg cgtccgaggt
     4141 gagcagtgcg cccgtctccg gctcgtgcac ccacagggtg ggcagcagcc gcatcatcgg
     4201 ggagatgagc tcgatgcgca tgccggggtg cggttcgatc cactgcgatg tgcccggctc
     4261 gacactcacg cgccggacct cggcgtgcga gcgcggcacg gtgatgcccc cggtgtacca
     4321 gaccgcgtcg atcgggaacc gtgactcgat ggccggcagg ttcaggcagc actccatctc
     4381 ggtacgggtg agcagcacgg cgagcgggac gccgccgtcg tacagctcct gcagggcggc
     4441 gagcacctcc tgctcgtggg tgcggatgcc cgtgtccacc agcagtgcac cggtctcgcc
     4501 gatgatgaga tagcagctga cgggctgcga ggcccgcacc cccggagggc accagctcac
     4561 tcgctcgtcg aggtcgatgt caccgccgag gcggtgcact gtggtggccg acatcagcgt
     4621 gccccgctca gcgagcggcg ctctcccaga ccgcgatggc ctcgtccagc gagcagtggt
     4681 catggcgggc gcgcatgacg gtcagcgccg cctcgtgcac ggtctcgtcg tacgcgccga
     4741 ccgcgtcgtc gatcagcacc gcgtagaagt cgttggagcc cgcatctcgc agcgtcgcct
     4801 cgacgcatcc gcctgtcgac accccgacga tcgcgaccga ctcgatgccc tggctgcgca
     4861 gcagcagggc cagctcggtg ccgacgaacg cgctctggcg gcgcttgcgg atgacgatct
     4921 catcgccttc gggctcgcat gcctcggcga actccgtgag cgggtccccc ttggcgctga
     4981 gcctgccgag gtcggtcgag cgcccctgac ccacggtgta cagggcccgc aaccaggccg
     5041 ccgtcgagct cttgccgtcg ggcaggttct ccacgcagat gtggaagatc ggcacgccgt
     5101 tcgctcgggc ggccctgatc gcgcgaccgc actggtcgac gatgcgcggc atcatgctca
     5161 tgtcgtggcc cgagtccgcg atggccccgc ccttggagac cgcgcccttc tgcatgtcga
     5221 tgacgaccag tgcggtcgtg gccgggtcca gcagttcagc gaaggtgtcg cgcacctgct
     5281 tgccgcagat ctcgatcatg tcgttctcct ttcagacccg gtgccggccg ggatcgctct
     5341 ccgaccggca ccggtgtgat gtcttctcgt cagccgccga gggcggcgaa gtagatctgg
     5401 ttgttggtga gcggattcgc cccggtcagg cccttctgcc gcgcgagcat gttcttggtc
     5461 tgcacgagcg cgaggatcgg cgagccctcg tcgatgtagt ccgcggcgtc ctgcagcagc
     5521 gtcttgcgat cggcctcgct ggtcttgacg tcggccatct tcagacgggc ctcgtcgaac
     5581 cgagggtccg cccagccggt gctgttgctc ggagcgccgc cggcgtagaa cgcgtagctg
     5641 tagaacgccg gatcgttgat gacgatcggc tcggagtaca ggatcgcctg atagcggcca
     5701 tcgatcacgg cggtggagaa gtccgtggtg ctcgcgatgt tcttcagtgc gacgcggatg
     5761 cccacgtcct tgagcatcga ctggatcagc accgccgagc gttcggccac cgggccggga
     5821 cgcgtgacgc tgtagctcag ttccatgtcg aagccgtccg ggtaaccggc atccgccagc
     5881 gccttcttgg ccgcggcctt gtcgaaggtg tagctcggag tcgccttcac gccggggaag
     5941 atcgctttcg acggctcacc gtagcccgag tacgcgccct tgaccagtgc agcacggtcg
     6001 atcgcctgcg agacggcgcg acgcaccgcg gggtcggcga ggggtccggt cttggtgttg
     6061 atcatgagcg tgtcgcgcgt ctgtccgacg cagtcgagca cctgcacgcc gggggccttc
     6121 gcgagcgagg cgtactggtc gtattcgaac gacccggcca cctgggcctc gccggactcg
     6181 acgagctggc tgcgggtggc tgtgttcgac acggccttga tgacgacgcg cttgacggag
     6241 ccgcgcttgc cctcccagtg cgagtcggcc ttcagcgtga gcgacgtgcc gggggtgaac
     6301 tcctcgaggt cccagccgga gtagtccgcg aggttcttgg agagccagtc gttggccgtc
     6361 gggtcgtccg cggtcgcgtg cttcttggcc tccacgctgt cgtggatgta gaacaggttg
     6421 ttctgcagcg acgcgagcgt gtacgaggtg acgaccttgg tgttcagctg gatggtgtgc
     6481 tcgtcgatga tcttggtgag gttgtcgacg tcgaacccgc tcgtggtcag cgtgcccttc
     6541 atgaccggct gacggaccat gccgatctga aggctccacg cgacgtcctc ggcagagagc
     6601 ttgttgcccc actggctggt cagatcgcgg agcttgacga tgacgccggt gccgtccggg
     6661 ctgagctcgg cgctctcggc cagacgcccg gtgaggtcgg tcgacggctt gaggcagctc
     6721 tcctcggagc cggcgccctt gtacgccatc agctgcgagc cgagaccgct gaggacgacg
     6781 tgggaggcgc tgccgcccca gggcgagggg gtcagactgt cgggaaccgc cccgacggcg
     6841 acgacgacgc tctcgggctt cgacgcggcc ggagccgtcc cgtcggacga cggtgtgccg
     6901 gagcaccccg tgacgaccag cgagaccgcc gcggcgactg cgccgaggag agccagacgt
     6961 gaatgcttca ttgcatgttc cgttctgatt tcgggtattg caggggtggg tgggggcgag
     7021 cggtgaccga cctcagtagc ggtaggcctg cttgtggccc gctcggatga tgggctcgac
     7081 atcgcggacg ttcgtgatga cgttcccgtg ggtgggtgcg acgatctcga cgccgagctc
     7141 atccacgagc gccaggacgc ggtcgtagac gcgatcggcg tcgttgttga atcgcgccca
     7201 gtacagtgcg ctgccggtga agaacgcggc gttctcgacg gcgatgggct gggagagctc
     7261 gtccgtggtg agcgcgcact cccccgggag gtgcagcgga tcctcgtcat cgagctcggg
     7321 acggtgcatg tagcagaacc cgtcggccgt gaacatgacc ttctcgcgcg acgcgaccgc
     7381 ccactgggtg ttcggcaggt cgcggatcat cgcttcgacg atgcgcacct cctggccacc
     7441 gccgaggtcg atcacgtcac cggggacgcg cgagttcagg ttcggcacga tctgcgggta
     7501 gaagaggtgg tagtcgtgca tgtcgccgac caccttgatg tcggggtact tctcgacgag
     7561 ccggggcagg ttgccggtgt gcggcagctc ggggtgcgtc gggaagacgt aggtgagcgg
     7621 ctcgtctccc agaagctcgt cgagcttggc ggagatggtc ggccagctgg acgggtttcc
     7681 ggtgtcgacg atcatcgacg cctcggtgcc cttgaccacg tatgccgagt tgtggccgtg
     7741 gatgacgcgg tccgcgaggt ggaagggcag gcaggcgccc atccagaaga cgcccggcct
     7801 gatctcccga gggaactcgg cggccatcgt ctcggtgatg atgttctcca tggcgatgtc
     7861 tcctaggcgg cgtcgtcggc cagtgcggcc agcatgaggt cgaggtggcg gcggacgctg
     7921 gcgcgcccgc ggatgacggc gccgcgcgag ggagcgatga cctcgggatc gaagcggtcg
     7981 aaggtctcca cgagccagtc gcgcagcggg tgcttggtcg cacggggaag ccactcgtac
     8041 ttggcgaaca gatgcgcggc gacctgcgcc ggcgtcgtgt cgtccttcac ggtgtcgtcg
     8101 atgaagcggt agccgtcggg acggtccatg accccatgcg tgaaggtgtc cgaggtgaac
     8161 agcgcgccgg tcttctcgtc ccagccccag aaggtgggga gcaggcgcag cagcggcgac
     8221 tggacgtcga ggatgcggcc gggagcgacc tcgatggtca tcgagcgctc gagcgcatcc
     8281 ccctcgacgg tgcgggtccc gtcgatctgc tggcggcgcc cgcggagggc catcctgctc
     8341 acgtcatcga acgcgtcgaa cgggtttatg acaccgccgg tgaagagccg gaggatgtcg
     8401 aagcggcgcg cgatgggctc gaggttggag acgcagtcca tctcggggcg ggtgaagaac
     8461 atcgagaccc cgccgccctc cccgatcagc tccgcgagat cgtcgagcac ctgctcgctg
     8521 tgcactgcca gtcccgagtc gatgaggagg tgatcctccc cctccgacag caggtagcag
     8581 ttcgacgcct ggtagccggt cgatccgcgt gggacccagc tgacgaggtc gctgagctcg
     8641 taggctccgc cgaggacggt cagtccgccg tcatgcactt tagtgaacat ggcttcaatc
     8701 tattgaagat ggatgatggt tgtcaagacc cgaggcacgg cttccgcagt cgcggagcgg
     8761 gctcagacgg ccgttgcgac ggcgggctgc gcggcaggct cacggcgacg cggcgcgacg
     8821 ttctgccgcg tacgcgggtc gagcagggtg atgagggtgt ccagcaccac gtagccgagc
     8881 aggctggtag cgcccatcac gagcacgaag ccctggatga ccggcacatc gcccttcagc
     8941 acgccgtcga gcgaccagcc cccgattccg ggccacgaga acacactctc gatgatcgcg
     9001 gcgccgctga gcgacgcggc gaacagcagc accatgtacg tcatcagcgc accgcgcaca
     9061 tcggtgagcg cgtaccggaa gacctgcgcg ggacgaaggc cgcaggcgcg ggcgaactcg
     9121 atctgcggcg accccaggat ctgcagcatc ccggtgcgca ccgtcttgcc gaacgccgcc
     9181 gcgaagaaca gcccgatggt caccgtcggc agcacggcac gactgaagat ggagcccacg
     9241 gtcccccact ggccggcgat cacggcgtcc accggaaccc agccggtcac cttcggcggc
     9301 ggcgagtcga gggcggagag catgcccagc ggcgccggcg cgaccttcag ttgctggtag
     9361 aagatgaaaa gcaggatcag cgcgagcacg aactccggca tcgccatgag gatcgacacc
     9421 accacgccga acgtgcggtc gggcaggcgt cccacgcggt acgcggcgac cgcacccatc
     9481 gccaggccga tgatcagcgc gaggatcagt cccgggacca ggtagacgag cgtgttgggg
     9541 agccgggtcc agagctcctc ggatactgag ctcccagtga agaacgacgt ccccaggtcg
     9601 ccgtgcagaa cgcgcaccag gtagtcgagg taccgctcga ggaagggctt gtccaggccc
     9661 agctgctcgt tgatcctggc gacgtcctgc ggggtggcga actcgcccag cagcgccttg
     9721 gcggggtcgc cgggcagcag ggagaccagc ccgaagctca gcgtgatcag tccccacagc
     9781 agcaggacgg cgatgatcag acgggagatg atgtagcgga tcatgattct cctgagacgt
     9841 cgaggtcgtc cagcacggcc gtgcgtccgc ggtacgggat cggcggcatg gccgcggcca
     9901 tgagattgcg cgtgtagtcc tcgaccgggg catccagcac ctgctgcgcg gtcccgccct
     9961 cgacgaaacg gccgtggtgc atcacgacga cgcgcgagca gaagtgccgc accgtctcga
    10021 tgtcgtgcga gatgaacacg taggtcagac cgcgagactc gcgcagccgg ttcagcatcc
    10081 gcagcgtcga cgcgcggatc gtgagatcga gggatgccgt cggctcgtcg agcacgacca
    10141 gcgacgggtc ggtgaccagc gcccgggcga tgccgacacg ctgctgctga ccgccactga
    10201 gattggcggg acgacgatcc aggaaatccg gcgagagccc gacctcctgc agcgtcgagg
    10261 tcacgcgttc gcgccgagcg gcacggccac cgtagccgcc gtgcacgatg agcggctcct
    10321 cgacgatctg gccgatggtc agacgcgggt tcagcgaggc gtacggctcc tggaacacga
    10381 tctgccagcg gtgacggtgg cggcgcagct cgcgtctgct gagcgcgcgg atgtcctgcc
    10441 cctcgagcac gacggagccg ctgtcggcgt cgtagagacg caggatcagc cggcccaggg
    10501 tcgacttgcc cgaccccgat tcgccgatga tgcccagaca ctcgccgtgc tccaccgaga
    10561 gcgagacccc gtccacagcg accttggcgc cgccgtgcgg cagggcgaag gtcttggtga
    10621 cgtcggtgag ttcgatcagt gccatctcag tactccatga ccttgggccg gacgccgacg
    10681 gtggcgatgt tcctggtgcc gcccgcgggc gactcctccg gacgcaccac ggagtccagc
    10741 agggacgccg tgtactcgtg ctccggtgtc acgaagacgc gggacaccgg cccctgctcg
    10801 acgatccgcc cctggtacat gaccaggacg cggtcgcagt agttggcgac gacaccgagg
    10861 tcgtgggtca cgatcagcat cgatcggccg ctgtcgacgg tcagcctggt gagggtgtcc
    10921 agcacctggc gctgcaccgt gaggtcgagc gcagtggtcg gctcgtccgc gatcacgagg
    10981 cgggggttga ggaacagcgc catggcgatg accacgcgct gcgccattcc gccgctgagc
    11041 tcgtgcgcgt agccgcggat gacgcgctcg gcgtccttca cgccagtggc ctcgagcagg
    11101 tccaccgcac gacggtgcag ttcctggccg cgcggcgcgc cgtgcgcctt ggcgatgttg
    11161 tcgaactgct tctcgatccg cagcaccgga ttcagcgcgc tgaaggggtt ctgcgcgacg
    11221 aatccgatct ccttgccgcg gatccggcgc agctcgcgct ccttaagatc caggagcgag
    11281 gcgccgtaga agtccacgtg tccctcggtc accttccccg ggggcatcag cagcttgatg
    11341 atggagcgga cgaccgtgga cttgccggaa ccggattcgc cgacgatgcc caccacctcg
    11401 ctctctccca cgccgaacga gacgccgtgc agcgcgcgga tggaggtcgt cctgttctcg
    11461 aagtcggtga ccaggccggt cacttccagg ccgctcatcg ggcacgctcc actcgctcga
    11521 tccggttctc gatcgcaccg gcgatcacgt tgaacgaggc gatcacgagc agcatcgcca
    11581 gggtggggaa cgcggcggcc caccactccc cgcggatcag catgctggct ccgtcgctga
    11641 tcatggcgcc ccaggtgggg acaggcgggt tcactccgac gccgaggaag ttcagcgcgg
    11701 cgatcgtgcc gagcgcgttc gccgcggtga gcgtggcctg cacgagcacc accccatagg
    11761 cgttgcggaa gacgtggttg aacgccacgc gcaggggcga gcatccgatg gcgacggcgg
    11821 cctcgacgta gcgggaggag cgcagggcga tggcggatgc ccgggacagg cgtacgaagc
    11881 gcggagcgcc gacgatcgcg atcgcgagga tgacgttcca ggcgccgccg cccatgagct
    11941 ggatcgcgac gatcgcgatg acgatgatcg gcagcgccgc gaaggcgtcg acgacccgca
    12001 tgaccgcgtc acccacccgc ccggtcgtgg cgaagaggcc gatgggcacg ccgacgacca
    12061 gcgcgagcag cgtggccgcc acggagatgg gcacgtcgag cttggcggcc tcgatcgtgc
    12121 gggcgaacac gtccatgccg ttgccgtccg tgccgaacca gtgcaccccg ttgggggcga
    12181 tcccgatcgc attcgggtcg gggcggaacg gatcgatcgg gtacgggccg atcagcgcga
    12241 gcaccggcag gccgatgatg acgatcacgg cgatggtcgc cgcgatcccg ttccagcgcg
    12301 cacccaggcg gagccggccc cgccgactcc agcgcactac gcggatcgac gccgatgtcg
    12361 gcggagcgga ctgcacgctc atgagaccct gctgcggtgt cgggcggctc cgggaagcgc
    12421 ggcggcctgc ggctgatctg acatgcggac tcctgtcgtg acctcgtcgt cgagatccgg
    12481 aagcggatca tcaacttatt gaagacatgt tcgacgcgag agtcaaccct tgatgtcgat
    12541 gacgcgcgta aatcctgccc tgaaaacccg agtacgccat ttcatcagaa tgaagagctt
    12601 catcgagcat tgacttcggc tgccctgatc ttcattatgg tgatgacaac acttcttgat
    12661 tgaagacagg atggtgcacg acgatgtccc ccactccctc ccccgtccgc tatctgctgc
    12721 acgacggcga gctcatcgac tacgcggacg cgcagctgca cgtgctcagc acgagcacga
    12781 agtacggcgt cggcgtcttc gagggcttcc gcgcgtactg gagcgaggag gacggcgagc
    12841 tctatgcctt ccgcgtcgag gatcacatgc gccggctgat cgactccatg cgggtcgtcg
    12901 agatcgacgg gcccaccgac accggcgtac tcgccgacca gctgatcgga ctcatccgcg
    12961 cgaacgagct gcgctcgaac ctgcacatgc gcacgcaggt gttcgtggac tcccccgacg
    13021 gcaagccgga cgacaccggt cccgcgacgg tgttcatggc cgcgatcccg atgggcaact
    13081 actttggcgc gacgggcctc gacgtgcaga tctccagctg ggcccgcctg tcggaccgcg
    13141 cgatgccgcc tcgggtcaaa tcgatcgcca actaccagaa cggacgcctc gccctgctgg
    13201 aggccaagcg caacggctac ggagcggcgc tgctgctcac cgagagcggt cacgtcgcgg
    13261 aaggtgcggg atacaacgtc ttcatggtgc gccagggccg gctctgcacg cccccgtcga
    13321 cggacagcat cctcgagggg atcacccgcg acagcgtgct gcagctggcg accacgcagc
    13381 tcggcttcga cgtcgacatc cgcccgatcg accgcaccga gctctactcg gccgatgaga
    13441 tcttcgtctg cggcagcgcc gcagaggtca accacgtcac ctccgtggat cgcacgaaga
    13501 tcggcgacgg cagcaccgcc ggtccgatca cggcgagcct gcagggcctg tacgcggatg
    13561 ccgtgcgcgg ccgcatcgcc gacttcggcc actgggtcac gccggtctac ggcaccgcct
    13621 cggcatgacc ggcacgacgg cgcacgccga cttcctgcac gcgcggggcg agttcgaccc
    13681 gggcgccgaa ccggtgctgt ccgtcgtgca gggtcagagg ttccgtctcg aggcgcggag
    13741 cgtgctcacc gatccgcgct tcgagacggc cgacgacttc tcgatcttct ccatccccgt
    13801 cacggggccc gtgcgcatcg agggcgtccg tcccggcgat ctggtccgca tcgatgtcca
    13861 cgacatccgt atcgccgatc gcggcggcat ggtcacgatg ccgggtcgcg gaggcttcgg
    13921 cgagcccctg aagatgcacg ggatggtggt cccggtgcac gacggcttcg cgcacttccc
    13981 ggatgacgtg cgcgtcccga tccgtccgat gatcggcaag atcggcttcg ccccagaggg
    14041 cgagagcccg aactcctcga ccgtgggccg ttacggcggc aacatggact gccgcgacat
    14101 catcaccggc tcctccctct tcatcaccgc gcaggtggac ggtggccttc tctacgcggg
    14161 cgacctgcac gccgtgcagg gcgacggcga gtgctcgctc accgccgtcg agatggcggg
    14221 ctcggtcgag ctgtcctgct gcgtcgtgca tcccgctccg gtgcgcggac cggtggtctt
    14281 cgccgaggat cgcatgatcg tgctcggcga cggcgaggat ctcgacgagg ccgccaccgt
    14341 cgcgctcgac gagatgatga cgatcatgcg cgccgaccgc ggatggaccc gcgagcgcac
    14401 cgccatgctc ctctccgccg ccgccgacgt cgccgtctcg cagctcgtca acgcccgcag
    14461 gagcgtgaag gtctccctcg acacccgcta cctgctcacc gctcccttcc agacgaaaga
    14521 ggactgacat gtacgacctc ctcatcaccg gtggcctggt ggtcaccccc tccggcgccg
    14581 agccgctgga catcgcgatc gccgacggcc gcatcgccgc cctcgccttc gccggcacca
    14641 cgccactcga ctctgccgcc cgagtgatcg acgtcggcgg caagctggtg gtgccgggcg
    14701 gcgtcgaccc gcacgtgcac acgaactccg tgctgcccac cgccgccgag agcgggatca
    14761 tgtgcttcgg agcggaccgg gtcagcgagg gcgccatcta cggcggcacc acgaccctgg
    14821 tcgacttcgc gcactggaag cccggcgacg acctctccga ctccttcgcc cgcaaggctg
    14881 cggagtggaa cggccagacc tacaccgact tcgctctgca cggcacgttc aaggagcccg
    14941 aggtcccgtt cagcgtgctg gagcagatcc cggatgccgt gcggaacggc cacggcagct
    15001 ggaaggtgtg gatgaccaac accaccccca cacgcccaca ccagaagacc gacctcggaa
    15061 acatgtgggg catcatggag cagaccgccg ccgcgggcgc catgctcgcc gtgcatgcgg
    15121 aggacgacga catcgtcatg tactcctacc gccgtctcaa gcaccagggc gcgatcggca
    15181 tcgagaacat gcaccacgcg cacaacgtgc tgtccgagca gctgtcgttc cagcgcgcga
    15241 tcacgctcgc gcgccacgtg ggcgccgcga tctacctgat gcacgtgagc gccaacgtcg
    15301 gcatcgacgc catccggaag gcgcgcggtg agggtgcccc gatctacggc gagatcctgc
    15361 cgcactacgc ctacttctcc gcggagaact accgcgagcc gcacggcgcg atctaccaca
    15421 cctatccgtc tctgaaggat gccgacgatc gcgaccacat gtggccggcg ctcatcgacg
    15481 gcacgctcag cacgctcgcc accgacggcg tgtgcacgga cttcgacgtg aagacgcgag
    15541 gcaacacgat ctacgacgcc acgggcggtc acgccggggt cgagatgcgg atggccgtgg
    15601 cgtacaccga gggcgtcgtg ggccgcggcc tcgacctcac ccgctatgtg gacctcacct
    15661 cggcgaacgc cgcgaagatc ctggggctgt atccgcgcaa gggcgcgatc gcggtcggca
    15721 gcgacgccga tctcgccgtg atcgacacgg acgtggaccg gcgggtcacc gccgcggacc
    15781 tgcacgaggc cgattacacg ccgtgggagg ggtaccgcac ccgcgcctgg aacatgatga
    15841 cgctgctgcg cggccaggtg atggtcgacg accgcgaact ggtggccggt ccggtcgggg
    15901 agctgcagag ccagcgcgtg tcgtcggacg tgctcagccg tccggcctgc tgagggatcg
    15961 cacatgacgg cgccggccgc tgcgccggtg cggatgctgg tgatcagcct gatggcgtcg
    16021 atgctcgtcg tctcgttcaa tcagaccgtg ctgaacaccg cgctgcccac catcatcgcc
    16081 gagctcggcg ggctggaccg catcggctgg atcgtggccg cgttcgtgct gacgtccaca
    16141 ctgagcatgc cgctggccgg tgcgctgtcc gacgtgctcg atcgcaagcg cctgctgctc
    16201 ggagcgagca tcgtgttcgg cctgggcacg ctgctcggcg cgctgtcctg gaatctcgac
    16261 gtgctcatca tcgcccggct cgcccagggc atcggcggcg gcggggtgat ggtgctgacg
    16321 cagacgatca tggccgcggc ggtcccctcc cgcgagcgcg ccaagtacgc cggcgccttc
    16381 ggttcggtgt gggcggtcgc gaccatcagc ggggtgctga tcggcggctg gctcaccgac
    16441 ggccccgggt ggcgctgggt cttctggggc acgctccccc tgctggtggt ggcgatggtg
    16501 ctgggcgcga ggttcatcgc gcccagcagg ccgagcgatg cccgcgtgca gcccgacatc
    16561 gccggcatgc tgctgctgtg catcgcgacg accgcgctgg tcacggccac ctccgcctcg
    16621 ctccccgccg agtggtcatg gttcatcggg gcggtgggcg ccggcgcggt gtgcgcgctc
    16681 gtgttcgccg agcgacgggc cgagcagccg gtgctgcccg gcatcctgtt ccgcagccgc
    16741 accttcgtcc tcgcgacact gtccgggatg ctgtgcagcg gcgtcgcctc gttcgtggtg
    16801 ctctcctacc tgccgtcgtt cgcgcagatg gtgctcgggc tctccgctct gcaggccggc
    16861 ctgctcctgc tgcccatgat cgtcgcgacg gtgacctcgt ccacgctgac cggcttcgcc
    16921 gtgtcgagga cgggtcggta catcctgatc ccggctctgg gggccggcgc cgtggtggtc
    16981 gggctgctgc tgctcgggac gctcggcccg gacacgaccc cgctccgcct ctcgctctcc
    17041 gcggcgctgc tgggcgcggg catcggcacg aacgtgctgc tgctgaccct gctggcgcag
    17101 aacgccctcc cggcacggaa catcggcgtg gcgacggcgg ccaacaactt cttccgccag
    17161 gcgggcgcga ccatggggat ctcgctggtc ggcgtcgcct tcgcctcgcg actgcacgac
    17221 gcactggcat ccgcactcga cacgcgcggc gtcccgcagc gcgaggatct gccgctggcg
    17281 acgatctctc ccgagcagct cgcgctgctg gaccccgggc tgcggtcggc cgtggcggag
    17341 gcatacgctt ccgcgctgcc accgctgttc ctggcgctca tccccctggc cctggcctcg
    17401 ctggtgatga tcctcgcgct cgagcggcgc cctctcgccg agtcgcacat ctgacgcacg
    17461 gccccacccc cgccggcgat ggggtacaaa cgaagagggc gtccgctccg cgggacggat
    17521 gcccacccgc atccgcccac tgaccgaggt cccctcatgt gccgcctgtt cgccttcgtc
    17581 tcccccgtca ccgccagcgc gcgcggagaa ctgggagcgg acggcatgga gagtctgctg
    17641 tcgctggcgc ggctgcacgg cgacggctgg ggccgtgcga gtgtgcgagc ccccggcgcc
    17701 gctccgatcg tgacccgctc ggcgatctcc gccgccgagg accccgactt cgatgcggcg
    17761 cttgcgacgc cctgtcgcgc agccgtggtg cacctgcgct gggcgaccgc cgggctgccc
    17821 gtgaacatcc ggaacgcgca tcccttcgag gcggacggca tcgcgttcgc gcacaacggg
    17881 acgatcaagc cgcgcgagac gcttcagggg ctgctctccg ccggcagcgt cgcagctctg
    17941 cagggcacga ccgacagcga gatgtacttc gcgctgatcc gcgaacgggt ctcggccgga
    18001 gagccgctcc ccgaagcagc ggcgcacgtc gcgcacacgc tgcgcgagct gttcccgctc
    18061 gccagtctca acgcgctgat catcgacgcc gagcacctcg tggtcgtgca cgccagtgcc
    18121 accagcatcc tcaccgagca tgacctggcg cgactggcgc cggtggcgga gctgctcccg
    18181 gacgagcaca acgaggacta cttcgctctg cgcatccggg agacccccga cggcacggtc
    18241 atggtcggat cgacgggcgt ggccggcctg gactgggagc cgctcccggc cgagtcggtc
    18301 atcgccatcc gcctcgacga ccggaggcgc tcgacgatcg ggatcgcctc cctcctcgcc
    18361 gcgcgatcct gacggagcat cgctagaatc gaggggtccg gccacggccg gtgcccgaaa
    18421 aaggggcacg aagcactctg cgaccgatcg aaggtcactt ccgatcgagt cccgcgatga
    18481 cgcgcgagcg gcgagacaca tacggacccc ctccgttcct ctcgaaaggc tgtcgcatgc
    18541 ccaccgtctc cgcgcacgtc gccctcaccc tcgcccagca catcgaccac gtcttcggcg
    18601 tgatgggcaa cggcaacgcc tacttcctcg acgcgatcga gacccagacg gatgccacct
    18661 tcaccgcggt ccgtcacgag cagggctccg tcgtcgcggc cgacgcgcac ttccgggcat
    18721 ccggacgcat cgccgcagcc accacgacct acggcgccgg tttcaccaac acgatcacct
    18781 cgctcgccga ggccgtgcag gcgcatgtgc cgctggtgct ggtggtcggc gacgagccga
    18841 catcgggccc ccgcccgtgg gatgtcgacc agatcgcgct cgcatcggcg gtcggcgcgc
    18901 gcacctacac gaccggccgc gcggatgccg cggcgaccac cgtcatcgcg atcgaacacg
    18961 ccctcaccta tcgggtgccc gtggtgctcg cgatccccta cgacgtcgcg gccctcgaag
    19021 cgggtcccgt tcccgaggcg cctgcgcccc gcatcccggc tcccctggct ccgcgcggcg
    19081 atctcgtcaa gcatcccgcc ggcgaa
//
LOCUS       DATMSX010000276         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_98914,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000276 DATMSX010000000
VERSION     DATMSX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98914"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN041_08415"
     CDS             complement(<1..578)
                     /locus_tag="VN041_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU29101.1"
                     /translation="MTQATLAPFPWSEVWGQDAAVETLQQAASDPAALSHAWLITGPP
                     GSGRSTLAYAFAAALITEHPDDENAMRQVLAGTHPDLTALRTDKVLITIKEARELVAR
                     SYFSPSAGRYRVIVVEDADRMVERTSNVLLKALEEPPEQTVWVLCAPSEADLLPTIRS
                     RVRSLRLREPDVADVARLITLRTRVDEAIAEQA"
     gene            complement(620..1249)
                     /gene="tmk"
                     /locus_tag="VN041_08420"
     CDS             complement(620..1249)
                     /gene="tmk"
                     /locus_tag="VN041_08420"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWU29102.1"
                     /translation="MTSERRGLWITLEGGDGSGKTTQAALLAEWLGAQGRTVLHTREP
                     GGSEVGVLIRNIVLHHRGEVAPRAEALLYAADRAHHVATVVRPALQRGDVVIQDRYLD
                     SSVAYQGAGRVLDATEVRDLSLWATEGALPDVTVLLDIDPSEARRRLDADDKPFDRLE
                     AEKEEFHTRVRDSYLRLAAAEPERFLVVDATRDPEDIASVIRARLSVLL"
     gene            complement(1246..4098)
                     /gene="topA"
                     /locus_tag="VN041_08425"
     CDS             complement(1246..4098)
                     /gene="topA"
                     /locus_tag="VN041_08425"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWU29103.1"
                     /translation="MAQGKKLVIVESPTKMRSIQGYLGDGYEVLSSVGHIRDLADKKD
                     IPAADKQAYGKYSIDVDHDFDPYYVVSDRKTKTVAELKRALKTADELLLATDEDREGE
                     AIAWHLLETLKPKVPVKRMVFHEITKDAIQAAVGRTRDLDLALVDAQETRRILDRLYG
                     WDVSPVLWYKVKSGLSAGRVQSAATRMIVDRERERMAFTSAEYWDVEAQASATGQSFG
                     VRLVRVDGGQLARGTDFDDTGKLKKAVVVLTEAEVMALAAAVNDAGAATVSKVEAKPG
                     TRSPYAPFTTSTMQQEAGRKLSMSAKQAMSVAQRLYEKGYITYMRTDSVALSTQAIQA
                     ARSQAVALYGDSAVPLKPRVYKSKSKNAQEAHEAIRPSGENFRTPASLSSQLDREEQR
                     LYDLIWKRTVASQMSDAKYETTTVTLESDADGKRLEFTASGTVYTFKGFLEAYEEGRD
                     EKRNAQDSAENQSLPAVAVGDELAMADAEAKGHRTTPKPRYTEASLVKALEEHGIGRP
                     STFASIIGTVIDRGYASKRGQALVPTWLAFSVVRLLEQHFADLIDYDFTAALEDDLDA
                     IARGEQNRNEWLRSFYFGSDAHVGLRQVVDNLGEIDARALNSTRITDTATLRFGKYGP
                     YLEVVDPANPDAKPRIVNVPEDLAPDELTPAKAQELVDAPVAGDRVLGENPENGKIVV
                     VKDGRFGPYVQENDPVSDEDEVDTETGEVVEAAPKKKSAKKKEAAPKPRTASLFRSMS
                     VETIDLETALQLLSLPRVVGKDPESGDEITAQNGRYGPYLKKGADSRSLESESQIFDV
                     TLEQALAIYAQPKYGAGSRRASSALKEFQADPVSGKPIRIRDGRFGAYVTDGETNVTI
                     PRGQTPDDVTFEIAVQMLADKRAKGPAPKRGAKKTTAKKAPAKKPAAKKAPAKKALTD
                     DQKAAARSEAAKKAAATRKANAIAKADAARKAKAGS"
BASE COUNT          580 a         1467 c         1571 g          762 t
ORIGIN      
        1 gcctgctcgg cgatggcctc gtcgacacgg gtgcggagcg tgatcagccg cgccacgtct
       61 gcgacatcgg gctcacgcag tcgcagcgag cgcactcgcg atcggatggt gggcagcagg
      121 tcggcctcgc tgggcgcgca cagcacccat acggtctgct cgggcggctc ctcgagcgcc
      181 ttcagcagca cgttggaggt gcgttcgacc atgcggtcgg cgtcctcgac cacgatcacc
      241 cggtagcgac cggccgacgg ggagaagtag gagcgggcga cgagctcgcg cgcctccttg
      301 atcgtgatga gcaccttgtc ggtgcgcagc gccgtgagat cgggatgagt gcccgccagc
      361 acctgccgca tggcgttctc gtcgtccgga tgctcggtga tcagcgccgc cgcgaaggcg
      421 taggcgagcg tggagcggcc ggatccgggc gggccggtga tcagccacgc gtgcgagagc
      481 gcggcaggat cggaggccgc ctgctgcagc gtctcgacgg ccgcatcctg accccacacc
      541 tcgctccagg ggaacggagc gagcgtggcc tgagtcatgc tgtccagcct aacctcggca
      601 cggacatcga cctgcgtgtt cagagcagga cggacaggcg ggcgcggatc acgctcgcga
      661 tgtcctcggg atcgcgggtg gcgtccacga cgaggaagcg ctcgggctcg gcggcggcga
      721 ggcgcaggta ggaatcccgc acgcgggtgt ggaactcctc cttctccgcc tccagacggt
      781 cgaacggctt gtcgtcggca tccaggcggc ggcgagcctc ggaggggtcg atgtcgagca
      841 ggaccgtgac gtcgggcagc gcgccctccg tcgcccacag cgacaggtcg cgcacctccg
      901 tggcatccag cacgcgcccg gcaccctggt acgcgaccga cgagtcgagg tagcggtcct
      961 ggatgacgac atcgccgcgc tgcagcgcgg gacgcaccac ggtggcgacg tggtgcgcac
     1021 ggtcggcggc gtacagcagc gcctcggcgc gcggcgcgac ctctccgcgg tggtgcagca
     1081 cgatgttgcg gatcagcacg ccgacctcgg aaccacccgg ctcacgcgtg tgcagcaccg
     1141 tgcggccctg cgcgccgagc cattcggcca gcagcgccgc ctgggtggtc ttccccgagc
     1201 cgtcgccacc ctcgagcgtg atccagagac cccgacgctc cgaggtcacg agcctgcctt
     1261 ggccttgcgc gccgcgtcgg ccttcgcgat cgcgttggcc ttgcgggtgg cggccgcctt
     1321 cttagcggcc tccgagcgcg cggccgcctt ctgatcgtcg gtcagcgcct tcttcgcggg
     1381 tgccttcttc gcagcgggct tctttgcggg ggccttcttg gccgtcgtct tcttcgcccc
     1441 gcgcttgggc gcgggaccct tggcccgctt gtcggcgagc atctgcacgg cgatctcgaa
     1501 agtgacgtcg tccggggtct gcccgcgcgg gatggtgacg ttggtctcgc cgtcggtcac
     1561 gtaggcaccg aagcggccgt cgcggatgcg gatcggcttg ccgctgacgg gatcggcctg
     1621 gaactccttg agcgcgctgg aagcccgccg cgaacccgca ccgtacttcg gctgtgcgta
     1681 gatcgccagc gcctgctcga gcgtcacgtc gaagatctgg gattcgctct ccagcgaccg
     1741 cgaatcagcg cccttcttca ggtacgggcc gtagcggccg ttctgcgcgg tgatctcgtc
     1801 gccggactca gggtccttgc cgaccacgcg gggcaggctg agcagctgca gcgcggtctc
     1861 gaggtcgatc gtctcgaccg acatggagcg gaacagcgag gcggtgcgcg gcttgggcgc
     1921 tgcctccttc ttcttggcgc tcttcttctt cggcgctgcc tcgacgacct cgcccgtctc
     1981 ggtgtcgacc tcgtcctcgt cggagaccgg gtcgttctcc tgcacgtacg ggccgaagcg
     2041 gccgtccttg accacgacga tcttgccgtt ctcggggttc tcccccagca cccgatcgcc
     2101 ggcgacgggc gcgtcgacca gctcctgtgc cttggcgggc gtcagctcgt cgggggcgag
     2161 gtcctcgggc acattgacga tgcgcggctt ggcatcgggg ttcgccgggt cgacgacctc
     2221 gaggtaaggc ccgtactttc cgaagcggag cgtggcggtg tcggtgatgc gggtggagtt
     2281 cagcgcccgc gcgtcgatct cgccgagatt gtcgaccacc tgccgcagcc cgacatgggc
     2341 gtcggagccg aagtagaagg agcgcagcca ctcgttgcgg ttctgctcac cacgggcgat
     2401 cgcgtcgagg tcgtcctcga gcgccgcggt gaagtcgtag tcgatcagat cggcgaagtg
     2461 ctgctcgagc agccgcacca cgctgaacgc cagccaggtc ggcacgagcg cctggccgcg
     2521 cttggacgcg taaccacggt cgatcacggt gccgatgatg ctcgcgaacg tcgacgggcg
     2581 gccgatgccg tgctcctcga gcgccttgac cagcgaggcc tcggtgtagc ggggcttggg
     2641 ggtggtgcgg tggcccttgg cctcggcatc cgccatcgcc agctcgtcgc cgacggcgac
     2701 ggcgggcagg gactggttct cggcggagtc ctgcgcgttg cgcttctcgt cgcggccctc
     2761 ctcgtacgcc tccaggaagc ccttgaaggt gtagacggtg ccggatgcgg tgaactcgag
     2821 ccgcttgccg tcggcatccg actccagcgt gaccgtggtg gtctcgtact tggcgtcgga
     2881 catctggctg gcgaccgtgc gcttccagat caggtcgtag aggcgctgct cctcgcggtc
     2941 gagctgggag gagagggatg ccggcgtccg gaagttctcg cccgatggac ggatcgcctc
     3001 gtgagcctcc tgcgcgttct tgctcttgga cttgtacaca cggggcttga gcggcaccgc
     3061 gctgtcgccg tacagcgcga cggcctggct gcgcgcggcc tggatggcct gcgtcgacag
     3121 cgcgaccgag tctgtacgca tataggtgat gtaccccttc tcgtagaggc gctgggcgac
     3181 gctcatcgcc tgcttcgcgc tcatcgagag cttgcgtccg gcctcctgct gcatggtgga
     3241 ggtggtgaag ggcgcgtagg ggctgcgggt gcccggcttg gcctcgacct tgctgacggt
     3301 cgcggcgccc gcgtcgttca cggccgcggc gagggccatg acctcggcct cggtcaggac
     3361 gacgacggcc ttcttcagct ttccggtgtc gtcgaagtcg gtgccgcggg cgagctgtcc
     3421 gccgtcgacg cgcaccaggc gcacaccgaa ggactgcccg gtcgcggagg cctgcgcctc
     3481 gacgtcccag tactcagccg aggtgaacgc catccgctcg cgctcgcggt cgacgatcat
     3541 gcgggtcgcg gccgactgca cgcgtccggc ggacaggccg gacttgacct tgtaccagag
     3601 cacgggtgag acgtcccagc cgtagagccg gtccaggatg cggcgggtct cctgcgcgtc
     3661 gacgagcgcc aggtcgaggt cgcgggtgcg gccgacggcc gcttggatcg cgtccttggt
     3721 gatctcgtgg aagaccatcc gcttgacggg caccttcggc ttgagagtct ccagcaggtg
     3781 ccacgcgatg gcttcgccct cgcggtcctc atcggtggcg agcaggagct cgtcggcggt
     3841 cttcagggcg cgcttcagtt cggcgacggt cttcgtcttg cggtcggaga cgacgtagta
     3901 ggggtcgaag tcgtggtcga cgtcgatcga gtacttcccg tacgcctgct tgtcggcggc
     3961 ggggatgtcc ttcttgtcgg cgaggtcgcg gatgtggccg acggagctga gcacctcgta
     4021 gccatcgccg aggtatccct ggatcgaccg catcttcgtc ggggactcga cgatgacgag
     4081 cttcttgcct tgtgccaaga gagcgtcctt tcttcgaagc acaccataca caccgctctc
     4141 ctggtgcgga cacagcgtcg catcgacacg gcgtcgtcgg gtgaccggag gcgggggtgg
     4201 gggtcgtcgt cctcatccct cgggttgccc ggcacgggcg cgggagacgg cgacgagacc
     4261 agcgtacgcg gattcgacct gcacggtcgc catgagaccg tccgcccggc acgcgacgag
     4321 cgtcgcgccg gctgccagcg ccacctgtgt cgcgagggcg caggggtcct cgccggtgac
//
LOCUS       DATMSX010000277         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_22949,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000277 DATMSX010000000
VERSION     DATMSX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22949"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..547)
                     /locus_tag="VN041_08430"
     CDS             complement(206..547)
                     /locus_tag="VN041_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HWU29104.1"
                     /translation="MARRIVHQLVDDIDGTLLEVGEGETVHFSLNGVAYEIDLTNSHA
                     EELRAALDPYINAGRRASAGTARAAAPRRRASRDSEVGEIREWAKAQGLKVSERGRIA
                     ADVMDAYRAAH"
     gene            complement(625..1473)
                     /locus_tag="VN041_08435"
     CDS             complement(625..1473)
                     /locus_tag="VN041_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWU29105.1"
                     /translation="MSVQTGSAEELVADGVRELARRTRFPVAFGGLIEDGEVAVTTIV
                     GNRTRHLDGLQVRPERGLGGRAMIELRPRMTNNYRASQQITHDYDVFVLGEGLRTLLA
                     LPIIVEGRPRGVLYAGAWEESEVGGVTTAPAMSVAQRIATELRIRDEVDRRLRESAPA
                     PALATPQREELRESFAELRSIAAGITDEALRDRIAAVERRLMGFVEPEQSATTGTIPT
                     VHLSPRELDVLACAAVGSTNSEIAAQLGLREGTVKAYLGTAMSKLDASTRHSAVARAR
                     RAGLLP"
     gene            1589..1936
                     /locus_tag="VN041_08440"
     CDS             1589..1936
                     /locus_tag="VN041_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWU29106.1"
                     /translation="MTDRIDEESGVGIDYIAVEESQRFQGLRRMQRSFIFPLAVFFLL
                     WYFAYVLLAAFAVDFMSQRVWGDITVGLLFGLGQFVTTFVITMWYVSFANRKLDPVAT
                     EIREELEKAEAGA"
     gene            1933..3552
                     /locus_tag="VN041_08445"
     CDS             1933..3552
                     /locus_tag="VN041_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156506.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HWU29107.1"
                     /translation="MNVTPLAAEAAKNDPVLNIAIFAAFVAVTLFIVIRASRNNKTAA
                     DYYAAGRSFSGTQNGFAIAGDYLSAASFLGICGAIAVNGYDGFLYSIGFLVAWLVALL
                     LVAEMMRNTGKFTMADVLSFRLKQRPVRMAAAITTLAVCFFYLLAQMSGAGGLVSLLL
                     GIDSKVGQSIVIAIVGVLMIIYVLIGGMKGTTWVQIVKAGLLIAGALAMTVWVLALHG
                     FSLNNLLQDAVANAPQGDKILGPGLKYGANPLDFLSLGMALVLGTAGLPHVLMRFYTV
                     PTAKEARKSVVWAIWLIGGFYLLSLVLGYGAGALVGPEAITKAPGGVNSAAPLLAQAL
                     GGPLLMGFISAVAFATILAVVAGLTITAAASFAHDIYSNVIKRGKDGTGESDAAGEVK
                     VARRTVIVIGILAIIGGIGAQGQNIAFLVALAFAIAASANLPTILYSLFWKRFNTRGA
                     VWSMYGGLIVAVALIVISPVFSGSPTAMIPGLDIAIFPLENPGIISIPAGFLLGWIGT
                     VTARTAESRALAAEMEVRSLTGFGAEKAVEH"
     gene            complement(3556..4164)
                     /locus_tag="VN041_08450"
     CDS             complement(3556..4164)
                     /locus_tag="VN041_08450"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWU29108.1"
                     /translation="MTFRHDFVPTPFGDALAVFSDDGIVSFDLSESADPSVPWLLEGI
                     SRRLGEVPDEAPGAGDEVAHLLDDYFEGAPIRFDDHVRFDWRLIDGFARAALQAVCDI
                     PWGETMSYGEVAVIAGSPGAARAVGTACRLTPFSVIVPVHRVIRSDGSPGHYGAHPER
                     KRFLLDLEREAASTSRTKRSPACRPPGSAPWGRPGRTGERPE"
     gene            4394..>5404
                     /locus_tag="VN041_08455"
     CDS             4394..>5404
                     /locus_tag="VN041_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU29109.1"
                     /translation="MNLGELSDSSTGIDIAHAAEARRTTLRAEAADLGGASPLVTFGD
                     TPEAGIDISKAHPGSLPQFITGRSTLLSNLFRDEVGLRTARMAAERITARNTELRTVR
                     GIDAVRLAVGIARWRIGGAAFSAPVLLRPLAIRRHHADFELKLQGGFEVNPELIRVAR
                     DHFGLTLDGPGLASLAYDGGIFKPQPVIDQLRALTHSIDTFAVHPRLVVSTFADVSGA
                     MVRDMVELDHPVLNALGGHAGDREQVSARREAPVVHGPDDRAPAAETLLLDADAEQEE
                     VLARIAAGHSLVVSTLPGTGGTQTVINALGAFVRAGKRVLVVSTRRSTLDGVRHRLLG
                     LQR"
BASE COUNT          818 a         1853 c         1849 g          884 t
ORIGIN      
        1 gtccttcgtc gtctagtctc tctcgacaga ggggccccgc cgggctgaga ggctccgcgc
       61 gccgcctctc agtccgacgt gacccgatct cgctgacgag cggcgtgtgg agtgcccggt
      121 ggggacgcga gggcacgacg cgcgacggca cgcatgatga aggccgatgc tccgaggagc
      181 atcggccttc tctgcattcc ggatgtcagt gcgctgcgcg atatgcgtcc atgacgtcag
      241 cggcgatgcg gccgcgttcg gagaccttca ggccctgggc cttcgcccac tcgcggatct
      301 caccgacctc ggagtctcgc gacgcccggc ggcgcggggc ggcggcgcgg gcggttccgg
      361 ccgaggcgcg gcgcccggcg ttgatgtacg ggtcgagagc cgcacgcagc tcctccgcgt
      421 gggaattcgt cagatcgatc tcgtacgcca cgccgttcag ggagaaatgg acggtctcgc
      481 cctctccgac ctccagcagc gtgccatcga tatcgtcaac gagctgatgc acaattcttc
      541 gagccatggg aagaacaata agtgcgatat tcccccctgg caagaatgcg cgcggtaaat
      601 cgcagcgaat tcatttcgga aatgtcaggg aagaagacca gcgcggcggg cgcgcgccac
      661 cgcggaatgg cgagtggagg catccagctt cgacatcgcc gttcccaggt acgccttgac
      721 ggtgccttca cgcagcccga gctgggcggc gatctcggaa ttcgtggagc ccacggcggc
      781 gcacgcgagc acgtccaatt cgcgcgggga gagatgcacc gtgggaatgg tcccggtggt
      841 ggctgactgc tcgggctcga cgaatcccat caggcggcgt tcgacggcgg cgatgcggtc
      901 gcgcagggcc tcgtcggtga ttccggcggc gatgctgcgg agctccgcga agctctcccg
      961 gagctcctct cgctgcggcg tcgccagagc gggcgcggga gcggactcgc gcagccgccg
     1021 gtcgacctcg tcccggatgc gcagctccgt ggcgatgcgc tgagcgacgc tcatggccgg
     1081 cgccgtcgtc acgcctccga cctcggactc ctcccatgcg ccggcgtaga gcactccgcg
     1141 cggacgcccc tcgacgatga tgggaagggc gagcagcgtg cgcagcccct cgcccagcac
     1201 gaacacgtcg tagtcgtgcg tgatctgctg cgaggcgcgg tagttgttcg tcatgcgggg
     1261 gcggagctcg atcatcgcgc gcccgccgag tcctcgctcc gggcgcacct gcagcccgtc
     1321 caggtgacgc gtgcgatttc cgacgatggt cgtgaccgcg acctcgccgt cctcgatgag
     1381 tccgccgaac gcgaccggga agcgcgtgcg ccgagcgagc tcgcgtacgc cgtcggcgac
     1441 gagctcctct gcggatccgg tctgcacgct cattctcagc aaataccaac tttcgggggt
     1501 gaccgctgcg tcctcccctt tcgtagcgtc gacgatacca cccacgtgac gccgtcacgt
     1561 ggcatcggac atgtggcaag gaggcctcat gacagatcgg atcgacgaag aatcaggcgt
     1621 aggcatcgat tacatcgcgg tcgaggaatc gcagcggttc caagggctga ggaggatgca
     1681 gcggtcattc atcttccctc tcgcggtgtt cttcctgctc tggtatttcg catatgtgct
     1741 gctcgctgcg ttcgcggtgg atttcatgag ccagcgggtg tggggtgaca tcaccgtcgg
     1801 cctgctcttc ggactgggcc agttcgtgac cacgttcgtg atcaccatgt ggtacgtctc
     1861 attcgccaac cggaagctcg acccggtggc gacggagatc cgcgaagagc tcgagaaggc
     1921 ggaggcgggc gcatgaacgt gacacccctg gccgcggaag ccgcgaagaa cgaccccgtc
     1981 ctgaacatcg ccatcttcgc ggcgttcgtc gcggtgacgc tgttcatcgt gatccgtgcg
     2041 agccgcaaca acaagaccgc cgccgactac tacgcggccg ggcgctcgtt cagcggaacg
     2101 cagaacggct tcgcgatcgc cggcgactat ctctcggcag catccttcct cggcatctgc
     2161 ggtgccatcg cggtcaacgg ctacgacggc ttcctctact ccatcgggtt cctggtggcg
     2221 tggctggtcg cgctgctgct ggtcgccgag atgatgcgga acaccggcaa gttcaccatg
     2281 gcggacgtgc tctcgttccg cctcaagcag cggccggtgc gcatggcggc cgcgatcacg
     2341 accctcgcgg tctgcttctt ctacctgctc gcgcagatgt cgggcgccgg tgggctggtc
     2401 tcgctgctgc tcggcatcga cagcaaggtc ggccagtcga tcgtgatcgc gatcgtcggc
     2461 gtcctgatga tcatctacgt gctcatcggc gggatgaagg gcaccacctg ggtgcagatc
     2521 gtgaaggccg gcctgctgat cgccggtgcc ctcgccatga cggtgtgggt gctggcgctg
     2581 cacgggttca gcctgaacaa cctgctccag gatgcggtgg ccaacgctcc gcagggagac
     2641 aagatcctcg gcccgggcct gaagtacggc gcgaaccccc tggacttcct ctccctcggt
     2701 atggcgctgg tgctcggcac cgcgggtctg ccgcatgtgc tcatgcggtt ctacacggtg
     2761 ccgacggcca aggaggcgcg caagtccgtg gtgtgggcga tctggctcat cggcggcttc
     2821 tacctgctga gcctggtgct cggctacgga gccggagcgc tggtgggacc cgaggccatc
     2881 accaaggcgc ccggcggggt gaactccgcc gccccgctgc tcgcacaggc tctcggcggt
     2941 ccgctgctga tgggcttcat ctcggcggtc gcgttcgcga cgatcctcgc ggtggtcgca
     3001 ggtctgacca tcacggcggc ggcatcgttc gcgcacgaca tctactccaa cgtgatcaag
     3061 cgcggcaagg acggcacggg cgagtccgac gccgcaggcg aggtcaaggt cgcgcgccgc
     3121 acggtcatcg tgatcggcat cctggcgatc atcggcggca tcggagcaca gggccagaac
     3181 atcgcgttcc tggtggcact cgcattcgcc atcgcggcgt cggcgaacct gccgacgatc
     3241 ctctactcgc tcttctggaa gcggttcaac acgcgcggcg cggtgtggag catgtacggg
     3301 ggcctgatcg tggcggtcgc gctcatcgtg atctcgccgg tcttctccgg aagcccgacc
     3361 gcgatgatcc cgggcctcga catcgcgatc ttcccgctgg agaaccccgg catcatctcg
     3421 atcccggcgg gcttcctgct cggctggatc ggcacggtga cggcgaggac cgcggagtcg
     3481 cgcgcccttg ccgccgagat ggaggtgcgc tcgctcaccg gcttcggtgc cgagaaggcg
     3541 gtcgagcact agtgatcatt cgggtcgctc cccagtgcga cccgggcgtc cccagggagc
     3601 ggagcccggc ggcctacacg ccgggctccg cttcgttctc gacgtcgacg cggcttcgcg
     3661 ttcgaggtcg agcaggaagc gcttccgctc cgggtgcgcg ccgtagtggc cgggggagcc
     3721 gtcggagcgg atcacgcgat gcacagggac gatcaccgag aagggggtga gccggcaggc
     3781 ggtgccgacc gcacgggccg cgcccgggct gcccgcgatg acggcgacct cgccgtagct
     3841 catcgtctcg ccccagggga tgtcgcacac cgcctgcagc gcggcacgcg cgaagccgtc
     3901 gatcagacgc cagtcgaagc ggacgtggtc gtcgaagcgg atcggcgcgc cctcgaagta
     3961 gtcatcgagc aggtgagcga cctcgtcgcc ggcgcctggc gcctcgtcgg gaacctcacc
     4021 cagtcgtcgg ctgatcccct cgagcagcca cggcacgctc gggtcggcgg actccgagag
     4081 gtcgaagctg acgatgccgt cgtccgagaa gaccgccagg gcgtcgccga agggcgtcgg
     4141 aacgaagtcg tgtcgaaagg tcatgccacc catcttcctg cgccgccgga cagcaggcgg
     4201 cgcggatccc ggcatccgtg gacaaccctt acggaaacct cccctgtgca gggaggatgt
     4261 tcacacgagt ccgccggcgc caatccgcgc gaatcggccc gggataccgg cccccgggcg
     4321 cgtcgcgctt aggctttcag atgtgtggcg acgagaggcg aagggcgccg aggacgcggg
     4381 agaggatgcc gcgatgaacc tcggggagct cagcgactca tcgacgggca tcgacatcgc
     4441 ccacgcagcg gaggcacgac gcaccacgct gcgcgccgag gccgccgatc tcggcggtgc
     4501 ctcgccgctc gtcaccttcg gcgacacacc cgaggccggg atcgacatct ccaaggcgca
     4561 ccccggcagc cttccccagt tcatcaccgg gcgctccaca ctcctgtcga acctgttccg
     4621 cgacgaggtg ggcctgcgca cggcgcggat ggcggccgag cggatcacag cgcgcaacac
     4681 cgagctgcgc acggtccgcg gcatcgacgc cgtgcggctg gcggtgggca tcgcgcgctg
     4741 gcggatcggc ggtgcggcct tctccgcccc cgtgctgctg cgcccgctgg cgatccgtcg
     4801 ccaccacgcc gacttcgagc tcaagctgca gggcggcttc gaggtgaacc cggagctcat
     4861 ccgtgtcgcc cgcgaccact tcggcctcac actcgacggc ccgggccttg catccctcgc
     4921 ctacgacggc ggcatcttca agccgcagcc ggtgatcgac cagctgcgcg ctctcacgca
     4981 ctccatcgac accttcgcgg tgcatccgcg gctggtggtc tccacgttcg ccgacgtctc
     5041 cggcgccatg gtgcgcgaca tggtcgaact cgaccacccc gtgctcaacg cgctgggcgg
     5101 gcatgcgggc gatcgcgagc aggtctctgc acgccgggag gctccggtcg tgcacggccc
     5161 cgacgatcgg gccccggccg ccgagacact gctgctggac gcggatgccg agcaggaaga
     5221 ggtgctcgcg cggatcgcgg ccgggcattc gctggtcgtc tccaccctgc cgggcacggg
     5281 cggcacgcag acggtgatca atgccctcgg cgcgttcgtg cgcgccggca agcgggtgct
     5341 cgtggtctcc acccggcgct ccacgctcga cggcgtccgc catcgcctgc tcgggctgca
     5401 acgg
//
LOCUS       DATMSX010000278         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_98994,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000278 DATMSX010000000
VERSION     DATMSX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98994"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VN041_08460"
     CDS             <1..918
                     /locus_tag="VN041_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylate synthase"
                     /protein_id="HWU29110.1"
                     /translation="LIVHGYRELLRDEALGGRVERAVVFGHPTLSREVAALLARADVD
                     VVAVRGGGEELDLNGSTTSVDAVSVADGPADRQWLGAWMNASAGGAIDLSAPAPDQDG
                     LSSEDKATRLSAVRAELDAVRRRVDREILADAVWRATWPHDRLVFGSSRLVRVADQVL
                     GGKKVPVHGNRGLAGIDGTIATALGIASASQVSGAVGVTRVLLGDLAFLHDVGALLLP
                     ADEPRPRIQVIVGNDGGGTIFDGLEVAASAPTEDLDRAFYTPHGANLRELAAAYGWDF
                     AQVTTRSALDQALTSPVAGPQIIEVPLER"
     gene            complement(943..>2187)
                     /gene="rmuC"
                     /locus_tag="VN041_08465"
     CDS             complement(943..>2187)
                     /gene="rmuC"
                     /locus_tag="VN041_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWU29111.1"
                     /translation="RRLAEVRDEAEADLADERRRAAERLEELKADQKRLSDEFDALSR
                     RALEANSKAFLEQAEERLKRSQSEGAAELQKRQDAVQQLIEPIQKTLDTVKTEMTTAE
                     KARLEAHAALSEQVQFMRQSSETLGAETRNLVNALRAPQVRGRWGELQLRRVVEASGM
                     VNHVDFDEQLHHSTDEGALRPDMVVHLAGDKRVVVDSKVAFNGYLEAMEATDDTVRAQ
                     RLAAHARHLKKHIDDLGTKEYWDVVAGCPEFVVMFVPAEPFLAAALDQDATLYEYAFE
                     RNVVIATPSTLIALLRTVGHAWRQDQLAQEAQQIFTVGKELHKRLGTLGQHLAKLGKS
                     LNSTVDAYNRFAGSLDRNVVTQARRFSALQGLDEVLTDTSPVEALAIAPSKADLYELE
                     APSADEERYASADVGEIAQRLD"
BASE COUNT          280 a          771 c          785 g          351 t
ORIGIN      
        1 ctcatcgtgc acggctaccg cgagctgctg cgggacgagg cactcggcgg ccgggtcgag
       61 cgcgccgtcg tcttcgggca tccgaccctc agccgagagg tcgcggcact gctcgcccgc
      121 gccgacgtgg atgtcgtcgc ggtgcgcggt ggcggcgagg aactggatct caacgggagc
      181 acgacctcgg tggacgccgt gtccgtggcc gacggaccag ccgaccggca gtggctgggc
      241 gcgtggatga acgcgtcggc cggaggggcg atcgatctga gcgccccggc gccggaccag
      301 gacgggctgt catccgagga caaggccacc cggctgagcg cggtgcgggc cgagctggat
      361 gcggtgcggc gccgcgtgga tcgggagatc ctcgcggatg ccgtgtggcg cgccacctgg
      421 ccgcacgacc gtctcgtgtt cggatcttcg cgactggtgc gcgtggccga tcaggtgctc
      481 ggcggcaaga aggtgcccgt gcacggcaat cgcgggctgg cggggatcga cggcacgatc
      541 gccacggcac tcggcatcgc ctcggcgagt caggtctcag gcgcggtcgg ggtgacgcgc
      601 gtgctgctcg gcgacctggc cttcctgcac gacgtcgggg cgctgctgct gccggcggat
      661 gagccgcggc cgcgcatcca ggtgatcgtc ggcaacgacg gcggcggcac gatcttcgac
      721 gggctcgagg tggcggcatc cgctcccacc gaggacctgg atcgcgcttt ctacacgccg
      781 cacggcgcga acctgcgcga gctggccgcc gcctatgggt gggatttcgc ccaggtgacc
      841 acgcgctccg cgctggacca ggcgctgaca tcgcccgtgg cggggccgca gatcatcgag
      901 gtcccgctgg agcgctgaac ctccgctccg accggccgcg gtctagtcga gccgctgggc
      961 gatctcgccg acgtccgcgc tggcgtaccg ctcctcatcc gccgagggcg cctcgagctc
     1021 gtacagatcc gccttggacg gagcgatcgc gagggcctcg accggtgagg tgtcggtcag
     1081 cacctcgtcc agcccctgca gcgcgctgaa ccggcgcgcc tgagtgacca cattgcggtc
     1141 cagggagccc gcgaaccggt tgtaggcgtc gaccgtgctg ttgaggctct tgcccagctt
     1201 cgcgaggtgc tggccgagcg tgccgaggcg cttgtgcagc tccttgccga cggtgaagat
     1261 ctgctgcgcc tcctgcgcga gctgatcctg ccgccaggcg tgcccgaccg tccgcagcag
     1321 cgcgatgagg gtggaggggg tcgcgatcac gacgttccgc tcgaacgcgt actcgtacag
     1381 ggtggcgtcc tggtcgagcg ccgcggcgag gaacggctcg gcgggcacga acatcaccac
     1441 gaactcgggg cagcctgcca ccacgtccca gtactccttg gtgcccaggt cgtcgatgtg
     1501 cttcttcaga tgccgggcgt gcgccgccag gcgctgggcg cgcacggtgt cgtccgtggc
     1561 ctccatggcc tcgagatagc cgttgaacgc aaccttcgag tcgaccacga cccgcttgtc
     1621 gccggcgagg tgcaccacca tgtcgggacg cagcgcgccc tcgtcggtgc tgtggtgcag
     1681 ctgctcgtcg aaatcgacgt ggttgaccat gcccgaggcc tcgaccacac ggcggagctg
     1741 cagctcgccc cagcggccgc gcacctgcgg cgcccgcagc gcgttcacga ggttgcgggt
     1801 ctcggcgccc agcgtctcgg aggactgccg catgaactgc acctgctcgc tgagcgcagc
     1861 gtgcgcctcc aaccgtgcct tctcggcggt ggtcatctcg gtcttcaccg tgtcgagcgt
     1921 cttctggatc ggctcgatga gctgctgcac ggcgtcctgc cgcttctgca gctcggcagc
     1981 cccttcgctc tggctgcgct tcagccgctc ctcggcctgc tccaggaacg ccttggagtt
     2041 ggcctccaga gcccggcggc tcagcgcgtc gaactcgtcg gagagccgct tctgatcggc
     2101 cttcagctcc tccaggcgct cggccgcgcg gcggcgctcg tcggcgagat cggcctccgc
     2161 ctcgtcccgc acctcggcga gccgccg
//
LOCUS       DATMSX010000279        12784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_127673,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000279 DATMSX010000000
VERSION     DATMSX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127673"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..775)
                     /locus_tag="VN041_08470"
     CDS             complement(197..775)
                     /locus_tag="VN041_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU29112.1"
                     /translation="MPGTTPTSELRRRWRASALPELRGVVLDLGAGSGIAADHLASGV
                     AWLALEPRRRIEPALRARLERRSGSRLLTAPAEAIPLESGSVDAVIASTVLCSVRDPA
                     RALEEVRRVLRPGGRLVFFEHVAGERGSWTSVLQALYAPLSRLIDHGCDPHRDTAASI
                     RAAGFASVEVKRMLAPGVLGTVEPLIEGVAVA"
     gene            complement(901..1656)
                     /locus_tag="VN041_08475"
     CDS             complement(901..1656)
                     /locus_tag="VN041_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU29113.1"
                     /translation="MTTPMTFTLVGGPTAVIEYAGLRMLTDPTFDPPGVYEGGIPLHK
                     LVGPALSPEEVGGIDVVLLSHDQHPDNLDHAGREFLSRASAVLSTPEAASRIAGVIGL
                     AAWEAHSVGDVEIVAVPALHGPEGAEALSGPVTGFLLRAPEQPTVYISGDNASVALVA
                     EIVAQVEVIDIAVLFAGAANVGRFGDSDLTLNARTAVAAARALGEAVIVPAHAEGWHH
                     FSETRERLVREFEYSGLAHRLRVPQHGVPLELS"
     gene            complement(1795..2697)
                     /gene="sucD"
                     /locus_tag="VN041_08480"
     CDS             complement(1795..2697)
                     /gene="sucD"
                     /locus_tag="VN041_08480"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWU29114.1"
                     /translation="MSIYLNKDSKVIVQGITGGEGTKHTALMLKAGTNIVGGVNARKA
                     GTTVTHDKPGSGEVELPVFGSVAEAIQKTGADVSVAFVPPAFAKDAMIEAIDAEIPLL
                     VVITEGIPVGDSAEAWAYAKSKGNKTRIIGPNCPGIITPGEALAGITPANITGTGPIG
                     LVSKSGTLTYQMMFELKDIGFSTAIGIGGDPIIGTTHIDALAAFEADPATKAIVMIGE
                     IGGDAEERAAEFIKAHVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSAGTAAAKKEALE
                     AAGVKVGKTPSEAASLMRGIIEAL"
     gene            complement(2719..3885)
                     /gene="sucC"
                     /locus_tag="VN041_08485"
     CDS             complement(2719..3885)
                     /gene="sucC"
                     /locus_tag="VN041_08485"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWU29115.1"
                     /translation="MDLYEYQARDLFEKYDVPVLAGIVADTPEEVKAAAEKLGGVVVV
                     KAQVKTGGRGKAGGVKVAKNPDEAYEAAKAILGLDIKGHIVKRVMVAAGARIAEEFYF
                     SVLLDRANRSYLSLCSVEGGMEIEQLAVEKPEALARVEVDPLVGIDKTKAIEIAKAAN
                     FPDELVSKVADVFVKLYDVYKGEGATLVEVNPLVRTEDGEIIALDGKVSLDENASEVR
                     HPEHEALEDKAAADPLEAKAKASGLNYVKLDGQVGIIGNGAGLVMSTLDVVAYAGEKH
                     GGVKPANFLDIGGGANAQVMANGLDVILGDPQVKSVFVNVFGGITSCVAVAEGIVKAL
                     EILGDSATKPLVVRLDGNQVEEGRAILAAANHPLVTSASGMDEGADKAAELANA"
     gene            4016..4675
                     /locus_tag="VN041_08490"
     CDS             4016..4675
                     /locus_tag="VN041_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29116.1"
                     /translation="MQNPAQLHTGVVAAALDLFAAQGFEATSVEQIAKAAGVSRSTFF
                     RQFGGKEDVVFTDHEQMLDDLRAFFEQPHEDPWAAVCEASERVFDYFVRDPDLARRRY
                     EIVRQVPVLRDREIVTVFRYERLFDEYLRDALPGLAPIDAVGFSALVTAVHNHVLRQL
                     LRGRKVPLSTLRTALDDVRRRYGVLPGGSDAAPDDVVVAVFPRSMPIAEISRRVQAEL
                     S"
     gene            complement(4708..5214)
                     /locus_tag="VN041_08495"
     CDS             complement(4708..5214)
                     /locus_tag="VN041_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29117.1"
                     /translation="MNELQGPTPAGTSRPYRSLAEALRAHRIPVENHEFIGAVTDAVG
                     VLSYIDRGRYIEALRREGAALHIGRTYINGFTEDEQITVAGRVLPLLPSEGRAPYFYV
                     NQPSGLPSLTVAAKRPAGTRTSAPRGAKPASAPRTPKATPVEERDYGVCDTCFMVRNA
                     AGKCGCDE"
     gene            5312..6346
                     /locus_tag="VN041_08500"
     CDS             5312..6346
                     /locus_tag="VN041_08500"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU29118.1"
                     /translation="MYEIPAPMLAKAAAAVPDSSKKPMLYEPKWDGFRGLVAWDGETL
                     EIGSRGAKPLTRYFPELVEQLPRILPGPCLLDGEIVVATGPAGAQRLDWEALSQRIHP
                     AASRVAKLSTETPAMFIAFDLLAEGEEDLQQHPFRERRARLERMLAGAASPLHLTRTT
                     EDAELARRWLAEFEGAGLDGVVAKPLDDPYAPGKRTLIKVKHARTADVVALGYRIHKS
                     GSGVGSLLVGLYGDDGVLRQVGGVAAWSDAMRAQLVEDLAPLVERDESGEAVVGEGER
                     SRFSGAKDVSFVRLRPERVLEVRYDQLEGARFRHTVQFERWRPDRDAHSCTYEQLDTV
                     AGYDLADVLA"
     gene            complement(6350..7438)
                     /locus_tag="VN041_08505"
     CDS             complement(6350..7438)
                     /locus_tag="VN041_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU29119.1"
                     /translation="MASERVTLTVTDADGEREISLSSPDKMVWPVEGGDGITKAEYAD
                     YVQQVSTPFLAANGHRPVSLERFRDGIASSDPDAAPGAGGFFSKNPPKGTPDFVDAVD
                     VTYNSGRSHPQIVLNRTSAIVWAVQMNTIVFHPWASLASNTDNPVELRIDLDPQPGTG
                     IAEAIVAAHGLREVLQEAGLEPFIKTSGNRGLHVFCPIVPEWEFLTVRHAVIAAGREL
                     ERRMPEKVTMSWWKEERGERIFVDFNQANRDRTMAGAYSPRAKVGAPVSTPIHWDELD
                     DVDPTRFTVRTIPGRLAEIGDPWADLQASPGRIDTLLEWWERDVSNGLGELSFPPEFP
                     KMPGEPPRVQPSKKVAANWDAEGNPVED"
     gene            complement(7482..8618)
                     /locus_tag="VN041_08510"
     CDS             complement(7482..8618)
                     /locus_tag="VN041_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HWU29120.1"
                     /translation="MALFSRRKKSVDQPDVETEAPVDDAIAQTDGVPEAPAESVPTVG
                     ISVQAFRGVGAEAGPEVSAPVESGTDAGEAVDEGIQMPRPASGAPVIAEEQMGGPRLP
                     LAPALPPEQTHTVDGLVDNVLLRTALAELEEGASNEQLLGVMRQSLQGHLYLRVHGDA
                     REQMAAGESLSVGVVRDDERTFMLAYSSGAALQASIQDEEDAASTSAIAQPATLVYQQ
                     VVSGGFTGIIIDNSSVPHRVVFPTELLQRALEQGDAEMSVKTLLAAPRDEQTPVRVAE
                     ALAKTRSWVAINDGSNGQPVGIAEAHTTDGRRFLQLFSHPLEVVVLGRNDQPMPFEPE
                     QLAKVLSEHDEIAGVLVDIAGPSIAIEREDLSAVMVLAVDLGDD"
     gene            complement(8676..11144)
                     /locus_tag="VN041_08515"
     CDS             complement(8676..11144)
                     /locus_tag="VN041_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU29121.1"
                     /translation="MTDAPLIVPASSGPRSSDGSDELLAGLNPQQLEAVTYRGNALLI
                     VAGAGSGKTSVLTRRVALLLRRREAWPSQILAITFTNKAAGEMRERVEGLVGDVARGM
                     WISTFHSACVRILRREAEQFGFTKSFTIYDSGDSRALLKRLVKEHEADAYGLTPGAVQ
                     SRISKLKNELLDAEGHARQANMSDPAERKFVDIFTDYQRALQKANAFDFDDLIAQTVF
                     LFRAFPKVANTYRRRFRHILVDEYQDTNHAQYSLIRELTKPVDTSAESGTAFESNGMM
                     IFEPDAPASGSEGEGASLTVVGDSDQSIYAFRGADIRNITEFERDFPGAKVVLLEQNY
                     RSTQNILSAANALIGNNFDRKAKNLWSDKGPGEKIIGFTGYSQHDEAQFVADEVEQLR
                     RSGVPYAEMAVFYRTNSQSRALEEIFIRAAVPYKIMGGTKFYERAEIKDALAYLVAVA
                     NPADEMAVRRILNRPRRGIGGVTEASIAMFAEQHGISFRDALRHPSELGVGPKLQASI
                     AQLDAVLSEAAEILQPSSGEVPPSTSVADGLTVLLERSGYFESLRSSRDPQDEARLEN
                     LDEFVAVARDFARNNPDGTIVDFLTEVALVSDADDLDDESGVVSLMTMHTAKGLEYDA
                     VFVTGVEEDLIPHRISAGEPGGPQEERRLFYVGITRARKRLHLSLAMTRAQFGEVSVA
                     MPSRFLQEIPADLIDWRQSPGDVNSRGGMQSRALNARKSGGWGSSGGGSGDRFGVKAL
                     PKRDSLKPLSTAMDRFPNKVTAKVRDNGDLELAAGDRIRHDDFGEGRVEAVTGEGAKR
                     IAHVRFDAAGQKKLLIKIAPIVKL"
     gene            11210..11584
                     /locus_tag="VN041_08520"
     CDS             11210..11584
                     /locus_tag="VN041_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HWU29122.1"
                     /translation="MRTILNIIWVVLGGFWHFLGYMLAGVILCIPIITIPWAIAAFRI
                     GKYSLWPFGRQVVDKPTSGLGSALGNVIWVVLAGWWLAIGHIVSGIAMCVTIIGIPMG
                     IADFKLVPVSLMPLGKEIVSSR"
     gene            complement(11589..12566)
                     /locus_tag="VN041_08525"
     CDS             complement(11589..12566)
                     /locus_tag="VN041_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWU29123.1"
                     /translation="MPRKSPLIIGHRGAPGYRPEHSRSSYELAFALGVDAVEPDVVAT
                     KDGVLVIRHENEISGTTDVAQHPEFADRRTTKRIDGEKLTGWFTEDFTWAELSTLRVR
                     ERLPRVRTSSATFDDAQPILRLPDLLEMISAASAEHGREIGLVLEVKHATYFEGIGLD
                     LVPLIERDLRAGGWAAGELPLVIESFESTVLDRLRETGIAASFIYLIEASGKPFDLVT
                     SMGPSAPDYAAAVTPEGLGALRADGISVNKRMLLQPGNTIVPDAHARGLRVFTWTCRP
                     ENQFLSPRFRAGGGKAAFGDYEGEWRVIADTGVDGVFVDHPDLGVAFFR"
BASE COUNT         1857 a         4378 c         4485 g         2064 t
ORIGIN      
        1 gatgcttccg cccgcgcgag cgcgcacgcc gcgctggaca agctgctgga cgccttcgcg
       61 gcctgacgca cgcggggtcc tcctctgccg cgcgacgccc attctgaagg acgtccgcag
      121 ttctgaagga tgcccggccg cgcagcatcc ttcagaggcg cgatcctcct tcagaagcgg
      181 atgccgctgc aggcggtcac gcgacggcca cgccctcgat gagcggctcc accgttccga
      241 ggacgcccgg cgccagcatc cgcttcacct cgaccgacgc gaagccggcg gcccggatcg
      301 aggcggccgt gtcgcggtgg ggatcgcagc cgtgatcgat cagccgcgac agcggggcgt
      361 agagggcctg gagcaccgag gtccagctgc cgcgctcccc cgcgacgtgt tcgaagaaca
      421 ccagccgccc gccggggcgc agcacccgtc gcacctcctc gagcgcgcgg gcggggtcac
      481 gcaccgagca gagcacggtc gaggcgatca ccgcgtcgac cgaaccgctc tcgagcggaa
      541 tcgcctcggc cggcgccgtg agcagacggg aaccgctccg gcgctccagg cgcgctcgaa
      601 gcgcgggttc gatcctgcga cgcggttcca gtgccagcca ggcgacgccg gatgccaggt
      661 gatccgccgc gatccccgat ccggcgccga ggtcgagcac gacgccgcgc agttcgggca
      721 gagccgaggc gcgccagcgg cggcgcagct cagaggtcgg cgtggtcccg ggcatccgct
      781 caggataggc acgattccgc cgcggcggaa cccgtgacgt ctgaaggtga tctgcgcttc
      841 tgaaggatct tccggcgccc ggcgtccttc agaggcgcga tcgtccttca gaagcgggga
      901 ttacgagagc tccagcggca cgccgtgctg cggcacgcgc agccggtgag cgagcccgga
      961 gtactcgaac tcccgcacga gtctctcgcg agtctcgctg aagtgatgcc atccctcggc
     1021 gtgcgccggc acgatgacgg cctcgcccag cgcccgagcc gcagcgaccg cggtgcgcgc
     1081 gttcagcgtg aggtcgctgt cgccgaagcg accgacgttc gcagcgcccg cgaacagcac
     1141 ggcgatgtcg atgacctcga cctgcgccac gatctcggcg acgagcgcga ccgacgcgtt
     1201 gtccccggag atgtagacgg tcggctgctc cggcgcccgc agcaggaagc cggtgaccgg
     1261 gccggagagt gcctccgcgc cctcaggacc gtgcagggcc gggacggcga cgatctcgac
     1321 gtcgccgacg gaatgggcct cccacgccgc cagtccgatc accccggcga tccgcgatgc
     1381 cgcctcgggc gtggagagca cggcactcgc ccgggacagg aattcgcgcc cggcatggtc
     1441 gagattgtcc ggatgctggt cgtgcgacag cagcacgacg tcgatgccgc cgacctcctc
     1501 agggctcagg gccggcccca cgagcttgtg cagcgggatg ccgccctcgt agacgccggg
     1561 aggatcgaag gtcggatcgg tcagcatccg gagccctgcg tactcgatca cggccgtcgg
     1621 gccgcccacc agggtgaagg tcatcggcgt ggtcatgtct ccatcctgcc ccggccagga
     1681 tctcctcggg ccggaatccg cgcacttccg aggagacccg tgcttctgcc gggtgcccgg
     1741 acatgacgaa ggccccgggg tggagcccgg ggccttcgag atacagctta cagctcagag
     1801 agcttcgatg atgccgcgca tcagcgaagc cgcctcggac ggcgtcttgc cgaccttgac
     1861 gccggcggcc tcgagggcct ccttcttggc ggctgcggtg cctgcggagc cagacacgat
     1921 ggcgccggcg tggcccatgg tcttgccctc gggagcggtg aagccggcca cgtagccgac
     1981 gaccggcttg gtgacgtgcg ccttgatgaa ctcggccgcg cgctcctcgg cgtcgccgcc
     2041 gatctcaccg atcatgacga tcgccttggt ggcggggtcg gcctcgaacg cggcgagcgc
     2101 atcgatgtgc gtggtgccga tgatcgggtc gccgccgatg ccgatggcgg tcgagaatcc
     2161 gatgtccttc agctcgaaca tcatctggta ggtcagcgtg cccgacttcg agaccaggcc
     2221 gatcggaccg gtgccggtga tgttggcggg cgtgatgccg gccagcgcct caccgggcgt
     2281 gatgatgccg gggcagttcg ggccgatgat gcgggtcttg ttgcccttgg acttggcgta
     2341 ggcccaggcc tcggccgagt cgccgacggg gatgccctcg gtgatgacca cgagcagcgg
     2401 gatctcggcg tcgatggcct cgatcatcgc gtccttggcg aaagccggcg gcacgaaggc
     2461 gaccgacaca tcggcgccgg tcttctggat cgcttcggcg acggagccga agacgggcag
     2521 ctcgacctcg cccgagccgg gcttgtcgtg cgtgaccgtg gtgccggcct tgcgggcgtt
     2581 cacgcccccg acgatgttgg tgccggcctt gagcatgagg gcggtgtgct tggtgccctc
     2641 gccgcccgtg atgccctgga cgatgacctt ggagtccttg ttgaggtaga tcgacatgtt
     2701 tctctcgtcc ttacaatctc aggcgttcgc cagctcggcg gccttgtcgg cgccctcgtc
     2761 catgcccgac gccgaggtga ccagcgggtg gttcgcggcg gcgaggatcg cacgcccctc
     2821 ctcgacctgg ttgccgtcca ggcgcacgac cagcggcttg gtggcggagt cgcccaggat
     2881 ctcgagggcc ttcacgatgc cctcggccac ggccacgcag gaggtgatgc cgccgaagac
     2941 gttgacgaac acgctcttga cctgcgggtc gccgaggatg acgtccaggc cgttggccat
     3001 gacctgcgcg ttcgcgccgc cgccgatgtc gaggaagttg gcgggcttca ctccgccatg
     3061 cttctcgccg gcgtaggcga ccacgtcgag cgtcgacatg accagacccg cgccgttgcc
     3121 gatgatgccg acctggccgt cgagcttgac gtagttcagg ccggatgcct tggccttggc
     3181 ctcgagcggg tcggccgcgg ccttgtcctc gagcgcctcg tgctcggggt ggcgcacctc
     3241 ggaggcgttc tcgtcgagcg agaccttgcc gtcgagggcg atgatctcgc cgtcctcggt
     3301 gcggaccagc gggttcacct cgacgagcgt ggcgccctcg cccttgtaca cgtcgtagag
     3361 cttgacgaac acgtcggcga ccttcgagac gagctcgtcg gggaagttcg cagccttggc
     3421 gatctcgatc gccttggtct tgtcgatgcc gaccagcggg tcgacctcga cgcgggccag
     3481 ggcctcgggc ttctccacgg cgagctgctc gatctccatg ccgccctcga cgctgcacag
     3541 cgacaggtag gagcggttgg cgcggtccag cagcacggag aagtagaact cctcggcgat
     3601 gcgggcgccg gcggcgacca tgacgcgctt gacgatgtgg cccttgatgt cgagaccgag
     3661 gatcgccttg gcggcctcgt acgcctcgtc ggggttcttg gcgaccttga cgccgcccgc
     3721 cttgccacga ccaccggtct tgacctgggc cttgacgacg accacgccgc cgagcttctc
     3781 agctgctgcc ttcacctcct caggggtgtc ggcgacgatg ccggcgagaa ccggcacatc
     3841 gtacttctcg aaaaggtctc gtgcctggta ctcgtagaga tccacagtgc agtccttcgc
     3901 tggttgcggc gggtgggacg cgacagttct gtcgggcgtc gatatcattc gacccgatcc
     3961 aaaagcctac taccgccgac gacatcacca gaacggccga gactaggctt ccactatgca
     4021 gaatcccgct cagctccaca ccggtgtggt cgccgccgcg ctcgacctct tcgccgccca
     4081 gggattcgag gcgacctcgg tcgagcagat cgccaaggcc gccggcgtct cgcgctccac
     4141 cttcttccgc cagttcggcg gcaaggagga cgtggtcttc acggaccacg agcagatgct
     4201 cgacgatctg cgcgccttct tcgagcagcc gcacgaggat ccgtgggcgg ccgtctgcga
     4261 ggcatccgag cgcgtcttcg actacttcgt gcgcgacccc gacctcgccc gccgccgcta
     4321 cgagatcgtg cggcaggtgc cggtgctgcg cgatcgcgag atcgtgacgg tgttccggta
     4381 cgagcgcctg ttcgacgaat acctgcgcga cgcgctgccc ggtctcgccc cgatcgacgc
     4441 ggtcgggttc tcagcgctgg tcaccgccgt gcacaatcac gtgctgcgcc agctgctgcg
     4501 cggacggaag gtgccgctct ccacactgcg caccgccctc gacgatgtgc gccgccgcta
     4561 cggcgtgctg ccgggcggat cggatgccgc acccgacgac gtcgtggtcg cggtgttccc
     4621 gcggtcgatg ccgatcgccg agatctcacg ccgggtgcag gccgagctgt cctgagcccg
     4681 gccgccccgg tcaggagagc gggctcgtca ctcgtcgcag ccgcacttgc ctgcggcgtt
     4741 gcgcaccatg aagcaggtgt cgcacacgcc gtagtcgcgc tcctccaccg gggtcgcctt
     4801 gggcgtgcgg ggagcggatg ccggcttcgc cccgcgcggt gcggaggtgc gggtgccggc
     4861 gggtcgcttg gccgcgacgg tgagcgacgg cagtccgctc ggctggttca cgtagaagta
     4921 cggcgcgcgg ccctcgctgg gcagcagagg cagcacgcgg cctgcgacgg tgatctgctc
     4981 gtcctctgtg aagccgttga tgtaggtgcg tccgatgtgc agcgcggcgc cctcgcggcg
     5041 cagcgcctcg atgtagcggc cgcggtcgat gtagctgagg acgccgacgg catccgtgac
     5101 ggcgccgatg aactcgtggt tctcgacggg gatccggtgc gcgcggagcg cttcggcgag
     5161 ggatcggtag gggcgggagg ttcctgcggg ggtcggaccc tgtagttcgt tcatcacctc
     5221 gattctctca tccggagatc caggtgatga cccggatcgg gcgggccccg cgctctcgac
     5281 gggaggcgtg gcgcggggca ggatggggag catgtacgag atccccgcgc cgatgctcgc
     5341 gaaggcggcc gcggccgtgc ccgacagctc gaagaagccg atgctgtacg agccgaagtg
     5401 ggacggcttc cgcgggctgg tcgcctggga cggcgagacg ctcgagatcg gctcgcgcgg
     5461 cgccaagccg ctgacgcggt attttcccga gctggtcgag cagctgccgc ggatcctgcc
     5521 gggtccgtgc ctgctcgacg gcgagatcgt cgtggcgacc ggccccgcgg gcgcgcagcg
     5581 cctggactgg gaggcgctca gccagcgcat ccatccggcc gcctcgcggg tggcgaagct
     5641 gtccaccgag acgcccgcga tgttcatcgc cttcgacctg ctcgccgagg gcgaggagga
     5701 tctgcagcag catccgttcc gcgaacgcag ggcacggctg gagcggatgc tggcgggtgc
     5761 cgcctcgccg ctgcatctca cccgcacgac cgaggacgcc gagctcgccc ggcgctggct
     5821 cgccgagttc gagggcgcgg ggctcgacgg cgtggtcgcc aaaccgctcg acgacccgta
     5881 cgcccccggc aagcgcaccc tgatcaaggt caagcacgcc cgcaccgcgg atgtcgtcgc
     5941 gctcggctac cgcatccaca agagcggctc gggcgtcggg tcgctgctgg tggggctgta
     6001 cggcgacgac ggcgtgctgc ggcaggtcgg cggcgtggcc gcctggtcgg atgccatgcg
     6061 agctcagctc gtcgaggacc tcgcacccct ggtcgagcgc gacgagtccg gcgaagcggt
     6121 cgtcggcgag ggcgagcgct cccgcttcag cggggccaag gacgtctcgt tcgtgcggct
     6181 gcgcccggag cgcgtgctgg aggtgcgcta cgaccagctc gagggcgccc gattcaggca
     6241 caccgtgcag ttcgagcgct ggcggcccga tcgcgacgcg cactcgtgca cctacgagca
     6301 gctggacacg gtcgccggct acgacctcgc ggacgtgctg gcctgagtct cagtcctcga
     6361 cggggttgcc ctcggcatcc cagttcgcgg cgaccttctt gctcggctgc acccgcggcg
     6421 gctcaccggg catcttgggg aactcaggcg ggaaggacag ctcgccgagg ccgttcgaca
     6481 cgtcgcgctc ccaccattcc agcagcgtgt cgatgcggcc cggcgatgcc tgcagatcgg
     6541 cccacggatc gccgatctcg gcgagccggc cggggatcgt ccgcaccgtg aaccgcgtcg
     6601 ggtcgacgtc gtcgagctcg tcccagtgga tgggcgtgga cacgggtgcc ccgaccttgg
     6661 cccgagggct gtacgccccg gccatcgtgc ggtcgcggtt cgcctggttg aagtcgacga
     6721 agatgcgctc gccgcgctcc tccttccacc agctcatggt gaccttctcc ggcatccgcc
     6781 tctccagctc ccgtccggcc gcgatcaccg cgtgacggac cgtgaggaac tcccactccg
     6841 gcacgatcgg gcagaacacg tgcagcccgc ggttgccgct ggtcttgatg aacggctcga
     6901 gacccgcctc ctgcagcacc tcgcgcaggc catgcgcggc gacgatcgcc tcggcgatgc
     6961 cggtgcccgg ctgggggtcg aggtcgatgc gcagctcgac cgggttgtcg gtgttcgagg
     7021 cgagcgatgc ccacggatgg aacacgatcg tgttcatctg aaccgcccac acgatcgcgc
     7081 tcgtgcggtt cagaacgatc tgcgggtggc tgcgcccgct gttgtaggtg acgtccaccg
     7141 cgtccacgaa gtccggcgtg cccttgggcg ggttcttcga gaagaatccg ccggctccgg
     7201 gcgcggcatc cggatcggaa ctggcgatgc cgtcgcggaa ccgctccagc gagaccgggc
     7261 ggtgcccgtt ggccgccaga aagggcgtgg agacctgctg cacgtagtcg gcgtactcgg
     7321 ccttggtgat gccgtcgccg ccctccacgg gccagaccat cttgtcagga ctggacaggg
     7381 agatctcccg ctcgccgtcg gcgtcggtca cggtcagagt cacacgctca gaggccatgc
     7441 gacgagcgta cggggtccgg gcggcctcgc gacagatctc gtcagtcgtc gccgaggtcg
     7501 acggccagca ccatgacggc gctcaggtcc tcgcgctcga tcgcgatcga ggggccggcg
     7561 atgtcgacga gcacgcctgc gatctcgtcg tgctcgctga gcaccttcgc gagctgctcg
     7621 ggctcgaagg gcatcggctg gtcgttgcgg cccagcacca ccacctcgag cgggtgcgag
     7681 aagagctgca ggaaccggcg gccgtccgtg gtgtgcgcct cggctatccc gacgggctgc
     7741 ccgttggagc cgtcgttgat cgccacccag ctgcgggtct tcgcgagcgc ctcggcgacg
     7801 cgcaccggcg tctgctcgtc gcggggagcg gcgagcagcg tcttgaccga catctcggcg
     7861 tcaccctgct cgagagccct ctgcagcagc tcggtcggga acaccacgcg gtgcggcacg
     7921 gacgagttgt cgatgatgat gccggtgaac cctccgctga cgacctgctg gtacaccagg
     7981 gtcgcgggct gcgcgatggc ggaggtggac gcggcatcct cctcatcctg gatggaggcc
     8041 tgcagagccg cgcccgagct gtacgcgagc atgaacgtgc gctcgtcatc gcgcacgaca
     8101 ccgaccgaga gcgactcgcc ggctgccatc tgctcgcgcg cgtcgccgtg cacccgcaga
     8161 tacaggtgtc cctgcagcga ctggcgcatg acgcccagca gctgctcgtt gctggcgccc
     8221 tcctcgagtt cggccagtgc ggtgcgcagc agcacgttgt cgacgagacc gtcgacggtg
     8281 tgggtctgct cgggcggcag cgcgggggcc agcgggagac gtggtccccc catctgctcc
     8341 tccgcgatga ccggggctcc ggatgccggg cgcggcatct ggatgccctc gtcgacggct
     8401 tcaccggcgt ccgtcccgga ctcgacggga gcgctcacct ccggaccggc ctcggcaccg
     8461 acgccacgga acgcctgcac ggagatgccg accgtcggca ccgactcggc gggagcctca
     8521 ggcacgccgt cggtctgcgc gatcgcatcg tcgacgggtg cttcggtctc gacgtccggc
     8581 tggtcgacgg acttcttacg gcgggagaag agggccatat gagccagcct aatcgccgac
     8641 agcggtgccg atgggtccgt gagcctgtcg aagggttaca gcttgacgat cggtgcgatc
     8701 ttgatcagca gcttcttctg cccggcggcg tcgaaccgca cgtgcgcgat gcgcttcgcg
     8761 ccctctccgg tgaccgcctc gacgcgcccc tcgccgaagt cgtcgtgacg gatgcggtcg
     8821 cctgccgcca gctcgagatc gccgttgtcc ctcaccttcg cggtgacctt gttcgggaag
     8881 cggtccatgg cggtcgacag cggtttgagg gagtcgcgct tgggcagcgc cttgacaccg
     8941 aaccggtcgc ccgagccgcc accggaggag ccccacccgc cggacttgcg ggcattcagc
     9001 gcacgcgact gcatgccccc acgggagttg acgtcgcccg gcgactgccg ccagtcgatg
     9061 agatcggccg ggatctcctg cagaaagcgg ctgggcatcg cgacggacac ctcgccgaac
     9121 tgggcgcggg tcatcgcgag cgagaggtgc aggcgcttgc gggcccgggt gatgcccacg
     9181 tagaacagcc gacgctcctc ctgcggacca cccggctccc ccgccgagat gcggtgcggg
     9241 atgagatcct cctcgacacc cgtgacgaac actgcgtcgt actcgaggcc cttggcggtg
     9301 tgcatggtca tcagggagac gacgccggac tcgtcgtcca gatcatcggc atccgagacc
     9361 agcgcgacct cggtgaggaa gtccacgatc gtgccgtcgg ggttgttgcg ggcgaagtcg
     9421 cgggcgaccg cgacgaactc gtcgaggttc tccagtcgcg cctcatcctg cgggtcgcgg
     9481 ctggagcgca gcgactcgaa gtagccgctc cgctcgagca gcacggtgag cccgtccgcg
     9541 accgaggtcg acggcgggac ctcgccggag gacggctgca ggatctcggc ggcctcggac
     9601 agcacggcgt ccagctgcgc gatcgacgcc tgcagcttcg gccccacgcc gagctcggac
     9661 ggatgccgca gggcgtcgcg gaagctgatg ccgtgctgct cggcgaacat cgcgatcgac
     9721 gcctcggtca cgccgccgat gccgcgtcgc ggccggttga ggatgcgccg caccgccatc
     9781 tcgtcggccg gattggcgac ggccacgagg taggccagcg cgtccttgat ctcggcgcgc
     9841 tcgtagaact tcgtgccgcc catgatcttg tacggcacag ccgcgcggat gaagatctcc
     9901 tccagcgcac gggactgcga gttggtgcgg tagaacacgg ccatctcggc gtacgggact
     9961 cccgagcggc gcagctgctc gacctcgtcg gcgacgaact gcgcctcgtc gtgctgcgag
    10021 tagccggtga agccgatgat cttctcgccc gggcccttgt cgctccacag gttcttcgcc
    10081 ttgcggtcga agttgttgcc gatcagggcg ttcgccgcgg acaggatgtt ctgggtggaa
    10141 cggtagttct gctcgagcag cacgaccttg gcgccgggga agtcgcgctc gaactcggtg
    10201 atgttgcgga tgtcggcacc acggaacgcg tagatcgact ggtcggagtc gccgaccacg
    10261 gtgagcgagg cgccttctcc ctcggaacca gatgccggtg cgtccggttc gaagatcatc
    10321 atgccgttcg actcgaacgc ggtgcccgac tccgcagagg tgtcgacggg cttggtgagc
    10381 tcgcggatca gcgagtactg ggcgtggttg gtgtcctggt actcgtccac gaggatgtgc
    10441 cggaaccgtc ggcggtaggt gttcgccacc ttcgggaatg cgcggaacag gaacacggtc
    10501 tgcgcgatga ggtcgtcgaa gtcgaacgcg ttggccttct gcagggcccg ctggtagtcg
    10561 gtgaagatgt cgacgaactt gcgctccgcg gggtcggaca tgttcgcctg gcgggcgtgc
    10621 ccctcggcgt cgagcagctc attcttgagc ttggagatgc gggactgcac cgccccgggc
    10681 gtgagtccgt aggcatccgc ctcatgctcc ttgaccaggc gcttgagcag cgcgcgcgag
    10741 tcgccggagt cgtagatggt gaacgacttg gtgaagccga actgctcggc ctcacgccgc
    10801 aggatgcgca cgcacgccga gtggaaggtg gagatccaca tgccgcgcgc gacatcgccg
    10861 acgagcccct cgacgcgctc gcgcatctcg cctgcggcct tgttggtgaa cgtgatcgcc
    10921 aggatctggc tgggccatgc ttcgcggcga cgcagcagca gggcgactcg gcgcgtgagc
    10981 acgctggtct tgcccgatcc ggcgccggcg acgatgagca gcgcgttccc gcggtaggtg
    11041 acggcctcga gctgctgagg gttgaggccc gcgagcagct cgtcggaccc gtcggaggag
    11101 cgcgggccgg aggatgcggg gacgatgagt ggagcgtcgg tcatggccct tcgagtctag
    11161 gcggcggctc cgacagcggc ctcccgagcg ggcgtgcgag aatcacggca tgcgcacgat
    11221 cctcaacatc atctgggttg tcctcggcgg gttctggcac ttcctcggct acatgctcgc
    11281 cggcgtcatc ctgtgcatcc cgatcatcac gatcccgtgg gcgatcgcgg cgttccgcat
    11341 cggcaagtac tcgctgtggc cgttcggccg ccaggtcgtg gacaagccga cgtcgggact
    11401 gggctcggcg ctgggcaacg tgatctgggt cgtgctcgcc ggctggtggc tggcgatcgg
    11461 ccacatcgtc tcgggaatcg cgatgtgcgt gacgatcatc ggcatcccga tgggcatcgc
    11521 cgacttcaag ctcgtccccg tctcgctgat gcccctcggc aaggagatcg tctccagccg
    11581 ctgacgcctc agcggaagaa ggccacgccc aggtcggggt ggtcgacgaa cacgccgtcg
    11641 acgccggtgt cggcgatcac ccgccactcg ccctcgtagt cgccgaaggc ggccttcccg
    11701 cctccggctc ggaaccgcgg cgagaggaac tggttctcgg gtcggcacgt ccaggtgaac
    11761 acccgcaggc cgcgggcgtg ggcatccggc acgatcgtgt tgcccggctg cagcagcatc
    11821 cgcttgttga cgctgattcc gtccgcgcgc agcgcgccca ggccttcggg cgtgacggcg
    11881 gcggcgtagt cgggcgctga cgggcccatc gaggtgacga ggtcgaacgg cttgccgctc
    11941 gcctcgatca ggtagatgaa ggatgccgcg atgcccgtct cccgcagccg gtccagcacc
    12001 gtcgattcga aggactcgat gaccagcggc agctctccgg cggcccagcc gccggcgcgc
    12061 aggtcgcgct cgatcagcgg gacgaggtcc aggccgatgc cctcgaagta ggtggcgtgc
    12121 ttgacctcca gcaccagacc gatctcccgg ccgtgctcgg cggaggccgc gctgatcatc
    12181 tcgagcaggt cgggcaggcg caggatcggc tgcgcgtcat cgaaggtcgc actggacgtg
    12241 cggacccgcg gcagccgctc cctgacacgc agggtggaca gctctgccca ggtgaagtcc
    12301 tcggtgaacc agcccgtcag cttctcgccg tcgatccgct tggtcgtgcg cctgtcggcg
    12361 aactccggat gctgcgcgac atcggtcgtg cccgagatct cgttctcgtg ccggatgacc
    12421 agcaccccgt ccttggtggc gaccacatcg ggttcgaccg cgtcgacgcc gagcgcgaag
    12481 gccagctcgt acgacgagcg actgtgctcg ggacggtagc cgggcgcacc gcggtgcccg
    12541 atgatcagag gggatttcct gggcacgctc tcagcgtaaa gcaccccgtt cataggcgac
    12601 cgccactatc gttgaggaaa gagttgaccg atccctcggg agtagaggcc acccaccatg
    12661 agcaatttcg tcttcaacac ccccgcgttc cagcagcagg atccgcggaa cgtcgccacg
    12721 taccccgggg cgccgcagca gcccggccag cagggccagt acgccccgcc gcccccgccc
    12781 ggcg
//
LOCUS       DATMSX010000280         6827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_13759,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000280 DATMSX010000000
VERSION     DATMSX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13759"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VN041_08530"
     CDS             <1..456
                     /locus_tag="VN041_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29124.1"
                     /translation="VAGCGQRRRRRVVRAAMTTGRKATGVSAWALGLLILLPVPMLGS
                     LASGGAMVAAYGTLSRQGQLAKANAAAARRWGALFLVLSTGLLLLQLVLGLIRLAAHT
                     DEASGFFPQGIPITLYVLVCLVHLAVVIAGTLRARRGEVVRLPFMRDTA"
     gene            453..1121
                     /locus_tag="VN041_08535"
     CDS             453..1121
                     /locus_tag="VN041_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWU29125.1"
                     /translation="MSRSVELPSGEVQVSTAWERPAGPSRGVVAIAHGAGARMEHPFL
                     VGFAGALRDAGFTTVRFNFPYSEAGRRMPGPASHAILTWRAMVAAAHTEAPGEPVFAC
                     GKSYGGRMASMAVAEGLEVDGLVYVGYPLHPPGRPEKPRVEHLPEVRPPQLFVEGTND
                     PFVQPLSQLEEAVASCQDARIAWFEGGGHSFEVKGRKRPADEVGASIAPAVVDFLTAV
                     MTVG"
     gene            1129..1518
                     /locus_tag="VN041_08540"
     CDS             1129..1518
                     /locus_tag="VN041_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HWU29126.1"
                     /translation="MAGFHHVELWVADSTGFGAWAWLLNRLGFTLAQEWAEGRSWSAG
                     DAYLTLTTSPNLTASEHDRRRPGLNHLAFHGGDRDAVDALMRTAAGYGWTPLYAERYP
                     HAGGPDHYAGWLENEDGFKVEVVADPA"
     gene            complement(1487..2146)
                     /locus_tag="VN041_08545"
     CDS             complement(1487..2146)
                     /locus_tag="VN041_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWU29127.1"
                     /translation="MPFSRMNSLVMLSAAAGLGALWLWAELVDRRASRRWLGGGRPAV
                     EAPERTHAVVVLGYGNSGERANLVNRFRVRAGVRSLDPRSENLLVFCGGPVHSSTSEA
                     VIMQRYAREHLGYVGPTALDTTSRSTRENIAHAIPLIEHADTIAIVSNAPHAEMGREI
                     LWSERPDLAVRLVRGAEHRLGEAPVLKILGALRATYAKRTALWRRSSRGITPDLRPPR
                     P"
     gene            complement(2185..3357)
                     /locus_tag="VN041_08550"
     CDS             complement(2185..3357)
                     /locus_tag="VN041_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cmx/CmrA family chloramphenicol efflux MFS
                     transporter"
                     /protein_id="HWU29128.1"
                     /translation="MPLALYALALAIFVMGTSEFILAGLLPAIATDLDVSIGTAGLLT
                     SAFAGAMVIGAPLMAAVSHRWPPRLTLVVCLLLFAGCHIVGALTPDFSLLLATRALSA
                     LANAGFLAVALSRATTLVPVDQRGRALSILLSGTTIATVVGVPAGTLIGTTLGWRTMF
                     WLLAALCVPAAIGVLRGITSAATRVGQAVSPGLRAEFSQLRRPRVILAMSLGALINGG
                     TFAAFTFLAPVVTEVADLTEGWISAALVMFGVGAFLGVATAGRLSDRRPGAVLGLGGP
                     LLLAGWIVSALVASFPVALIVLVFVQGFLSFGVGSTLIAQVLHAASGAPTMGGSYATA
                     ALNIGAAAGPVLGAFGLTTRAGLLAPFWIAAALTGIALVVLLVAARTREDETAAAR"
     gene            3624..4049
                     /locus_tag="VN041_08555"
     CDS             3624..4049
                     /locus_tag="VN041_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU29129.1"
                     /translation="MLIAPNAEGTAHAVSLNAPTPENPAGYHRLIGGGVEFGETHEAA
                     IRREVDEELSAAIRELRFLGSIENIFQIDGVIGHEVVFVYTGRLDPEPALRGATLIES
                     DGSVVPVVWRPLADDDEQLPVYPEGALRLLAATLDETRA"
     gene            complement(4123..5010)
                     /locus_tag="VN041_08560"
     CDS             complement(4123..5010)
                     /locus_tag="VN041_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29130.1"
                     /translation="MESLLEIDSTRLFVDQRGPEDGHPLLFIHGGPGNPCWDFMASVG
                     DLFAARGIRIIGVDQRGVLRSDDLPEAEPLTVALLVQDFEGIRRRLSIERWTIIGHSS
                     GGGYALEYALTHPEHVSGLILDCPALDADATDRHRLPRAAAMLDDDGNADAAEVCRTL
                     AGLERRLTADDRTWEAMLPLGPRYLDLFFHDAATRALYEDVMEAAPKGLDWAKGMSHL
                     ALMAEMYRDRRPSLASLTVPSVLIHGEDDMVAPPVVRDAYRASTGGEIITIPDAGHFA
                     FVEQPETYVDVVTRFLGTL"
     gene            5091..5687
                     /locus_tag="VN041_08565"
     CDS             5091..5687
                     /locus_tag="VN041_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWU29131.1"
                     /translation="MIRAVVFDLDGVLRRFDPAHVAAIEARHGLLDGSIHAAAFAPEM
                     LDPVITGRISRAAWVRTVGEQLAHPQAAEEWGRHPSVRDAGMLALVDELRSRGIRTAI
                     LTNGTDTIRAELRESGIDVHVDEIFNSAEIGVAKPDPRAFQHVLDALDLPASEVFFTD
                     DSSSKLTGAEALGIHTHHFTGEVALRAALCDAGILVSR"
     gene            complement(5677..6315)
                     /locus_tag="VN041_08570"
     CDS             complement(5677..6315)
                     /locus_tag="VN041_08570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWU29132.1"
                     /translation="MSSAGLIIFDLDGTLVDQEAAAGRWADEFIRTYGADPALRTWIT
                     GELTARTPKDQVFPRVVEHSGARVDPSALWHEYRRRMPELVELFPGVVGALRDLRAAG
                     WMLGIATNGIADNQVGKIERTGLDVLVDGWVVSSEVGIRKPDPGLLIALAERVGVPLR
                     GWMVGDGLETDVEAGRRAGLRTAWITDRPRTDTAADLVVSDVPGFAVAVLSD"
     gene            complement(6312..>6827)
                     /locus_tag="VN041_08575"
     CDS             complement(6312..>6827)
                     /locus_tag="VN041_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWU29133.1"
                     /translation="ALILALRAVPVGARVFDGRGRGGSTQDHDEWIAHCITQSEGLVD
                     VSGVSRAWAELREVPQAGEHVMSHRDLTPLNLLMSHGRLAGVLDGGDFGPADRSLDLV
                     CAWHLLSEGPRQHLREVVGASDAEWRRGAAWALQQAMGLVWYYEASNPVMAELGRSTV
                     SRVLNDQDVIG"
BASE COUNT         1100 a         2369 c         2356 g         1002 t
ORIGIN      
        1 gtcgccggat gcgggcaacg gcgccgacgg cgagtcgtcc gagccgcgat gaccaccggg
       61 cggaaggcga ccggcgtctc cgcgtgggcg ctgggactgc tgatcctgct cccggtgccg
      121 atgctgggat ccctcgcctc cggtggtgcg atggtggccg cgtacggcac gctgtcccgg
      181 caggggcagc tggccaaggc gaacgcggcg gcggcgcggc gatggggtgc gctcttcctg
      241 gtgctctcga ccgggctgct gctcctgcag ctcgtgctcg gcctgatccg gctcgcagcg
      301 cacacggacg aggcatccgg gttctttccc cagggcatcc cgatcacgct gtacgtcctg
      361 gtctgcctgg tgcacctggc ggtggtgatc gccggcacgc tgcgcgctcg ccgcggtgag
      421 gtcgtccgtc tgccgttcat gcgggacacg gcatgagccg ttcggtcgag ctgccttccg
      481 gagaggtgca ggtgagcact gcctgggagc gcccggcggg tccgtcacgc ggggtcgtcg
      541 cgatcgcgca cggggcgggc gcacggatgg agcacccgtt cctggtcggc ttcgccggcg
      601 cgctgcgcga tgccgggttc acgaccgtgc gattcaactt cccctactcc gaggcgggca
      661 ggcggatgcc cggcccggcg tcgcacgcga tcctgacctg gcgtgcgatg gtggctgccg
      721 cgcataccga ggcgccgggc gagcccgtct tcgcctgcgg caagtcgtac ggcggcagga
      781 tggcgtcgat ggcggtcgca gaggggctgg aggtcgacgg actcgtgtac gtcggctatc
      841 ccctgcatcc gcccggcagg ccggagaagc cgcgggtgga gcacctgccc gaggtgcgac
      901 ctcctcagct gttcgtggag gggacgaacg atccgttcgt gcagccgctg tcgcagctgg
      961 aggaggccgt ggcgtcgtgc caggacgccc ggatcgcctg gttcgagggc ggcggtcact
     1021 cgttcgaggt gaaggggcgc aagcgcccgg ccgacgaggt cggcgcatcg atcgcgccgg
     1081 cggtcgtgga cttcctgacc gcggtcatga cggtggggtg aagcgagcgt ggccgggttc
     1141 caccatgtcg agctgtgggt cgcggactcc accgggttcg gtgcctgggc gtggcttctg
     1201 aaccggctcg gtttcacgct cgcccaggag tgggcggagg gacgctcctg gagtgccgga
     1261 gacgcctacc tcacgctcac cacgtcgccg aacctgacgg cgtcggagca cgaccggcgc
     1321 cggcccgggc tgaaccacct ggcgttccac ggaggtgatc gcgacgcggt ggacgcgctc
     1381 atgcgcacgg cggccgggta cggatggact ccgctctacg ccgagaggta cccgcatgcc
     1441 ggcggtcccg atcactatgc cggctggctc gagaacgagg acgggttcaa ggtcgaggtg
     1501 gtcgcagatc cggcgtgatg ccccgggagc ttcgacgcca cagcgctgtg cgcttggcgt
     1561 aggtcgcgcg cagcgccccg aggatcttca ggacgggggc ctcgccgaga cggtgctcgg
     1621 cgccccggac gagccgaact gcgagatcgg ggcgctcgct ccagaggatc tccctgccca
     1681 tctcggcgtg cggggcgttc gagacgatgg cgatcgtgtc ggcgtgctcg atgagcggga
     1741 tcgcgtgcgc gatgttctcg cgcgtggagc ggctggtcgt gtccagcgcc gtcgggccga
     1801 cgtagccgag gtgctcgcga gcgtagcgct gcatgatgac ggcttcggac gtgctgctgt
     1861 gcacgggccc gccgcagaaa acgagcaggt tctcggaccg gggatcgagc gatcggacac
     1921 cggcccgtac ccggaagcgg ttcacgaggt tggcgcgctc tccgctgttg ccgtagccga
     1981 gcaccacgac ggcatgcgtg cgctcgggtg cttcgaccgc gggtctcccg cccccgagcc
     2041 agcgcctgct ggcacggcgg tcgacgagct cggcccagag ccagagggcg ccgaggccgg
     2101 ctgccgccga cagcatgacc aacgagttca tccggctgaa gggcacgtgg ccaccatagc
     2161 cgccggtcgg tgtcgagaaa cggatcaccg ggctgccgcc gtctcatcct cgcgcgtacg
     2221 ggcggcgacg agcaggacga cgagagcgat ccccgtcagt gccgcggcga tccagaacgg
     2281 ggcgagcagc cctgccctgg tcgtcaaccc gaaagcaccg agcacgggcc cggcggcagc
     2341 tccgatgttc aacgccgcgg tcgcatacga gccacccatc gtcggcgcac cggacgctgc
     2401 gtgcagcacc tgagcgatca gggtgctgcc gacgccgaag gacagaaagc cctgaacgaa
     2461 gacgagcacg atgagtgcga ccggaaacga cgcgaccagt gccgacacga tccagcccgc
     2521 caacagcaga ggtccgccca gcccgagcac ggcgccgggg cgtcgatccg acagccgccc
     2581 cgcggtcgcg acgccgagga atgctccgac gccgaacatc accagtgcgg ccgagatcca
     2641 gccctcggtc aggtccgcga cctccgtcac gaccggtgcg aggaatgtga acgccgcgaa
     2701 ggtccctccg ttgatcaacg ctcccaacga catcgccagg atgactctcg gtcgcctcaa
     2761 ttgactgaac tcggcacgga ggccgggtga caccgcctgg ccgactcgtg tggcggcgct
     2821 cgtgatgcct cgaaggaccc cgatcgccgc aggaacgcag agggcggcca gtagccagaa
     2881 catggttcgc caacccagcg tggtaccgat cagggttccg gccggcaccc ccacgaccgt
     2941 cgcgatcgtc gtgccggaga gcaggatcga caatgcccgt cccctctgat ccacagggac
     3001 gagagtcgtc gccctgctca gggcgacggc caggaatcca gcgttcgcaa gagcgctgag
     3061 tgcgcgtgtg gcgaggagga gcgagaaatc cggcgtcaac gcgccaacga tgtggcagcc
     3121 cgcgaacagg agaaggcaga cgaccaacgt gaggcgcggc ggccagcggt gagagaccgc
     3181 cgccatcagt ggcgcgccga tgaccatggc acccgcgaaa gcagaggtca gcagacctgc
     3241 cgtgccgatg gagacgtcga gatccgtcgc gatggcgggc agcagcccgg cgagtatgaa
     3301 ttcggaagtg cccatgacga agatggccag ggcaagggca tagagagcaa gaggcatcga
     3361 ggactccgag gtgagagatc aagaaacggt tcttcttgtc accgcggtca gtgccccgcg
     3421 agtcggacat acccccacac ggactgtggg cgtgatgact agtgcttcaa ggggctgacg
     3481 gcgggaccga caacccctga cctgtctgac tcgggactcg acataccttg aacagtacag
     3541 tgctcgactg catccggtcg cggcggccct gccgatagcc tgatcggatg ccgatagatc
     3601 gaagcgtcat ccgcgtgaag gccatgctca tcgcgccgaa cgcggagggc actgcgcacg
     3661 ccgtcagcct caacgccccg acacctgaga atcccgcggg atatcaccgc ctgatcggcg
     3721 gcggtgtcga gtttggcgag acgcacgaag ccgccattcg acgagaagtc gacgaggagc
     3781 tgtccgccgc gatccgcgag cttcgctttc tcggatcgat cgagaacatc ttccagatag
     3841 acggggtgat cggccacgag gtggtcttcg tctacaccgg acgcctcgat ccggagccgg
     3901 cgctccgcgg cgcgacgctg atcgagagcg atggaagtgt ggttcccgtc gtctggcggc
     3961 ccctcgccga cgatgacgag cagctgcctg tctatcccga aggagctctc cgcctgctcg
     4021 ctgcaaccct ggacgagacg cgcgcctgac gaccaccccg tcgcgtcgga aggtgcctgc
     4081 tcgccggggc acgctgctga cgccgacgtc ggactgcgcg cgtcacaacg tcccgaggaa
     4141 tcgcgtgacg acgtccacgt aagtctccgg ctgctccacg aaagcgaaat ggccggcgtc
     4201 ggggatcgtg atgatctccc ctccggtcga cgcgcggtag gcgtcgcgca cgaccggcgg
     4261 cgcgaccatg tcgtcctcgc cgtggatcag caccgacggc acggtcagcg acgcgagcga
     4321 cggccggcga tcgcggtaca tctccgccat cagcgccagg tgagacatcc ccttcgccca
     4381 gtcgaggccc ttcggagccg cctccatcac gtcttcgtag agtgcgcgcg tggccgcgtc
     4441 gtggaagaag aggtccaggt agcgcgggcc cagcggcagc atcgcctccc aggtgcggtc
     4501 gtccgcggtc aggcgacgtt ccagacctgc gagcgtgcgg cacacctctg cggcatcggc
     4561 attgccgtca tcgtccagca tcgccgctgc cctcgggagg cggtgccgat cggtggcgtc
     4621 ggcgtccagc gccggacagt ccaggatcag gccgctgacg tgctcgggat gcgtcagcgc
     4681 gtattcgagc gcgtacccgc cgccggagga gtggccgatg atcgtccagc gctcgatgct
     4741 gagccgacgg cggatcccct cgaagtcctg cacaagcagg gcgaccgtca gcggttcggc
     4801 ctccggcagg tcatcggatc tcagcacgcc gcgctgatcg acgccgatga tgcggatgcc
     4861 cctggcggcg aacagatcgc cgaccgacgc catgaagtcc cagcacgggt tgcccggtcc
     4921 gccatggatg aacagcagcg gatgcccgtc ctcgggacca cgctggtcga cgaagaggcg
     4981 ggtgctgtcg atctcgagaa gggactccac cccagcaagc tacggtgccg cagtcgcttc
     5041 ggaaaggggc cttgtacatc tgcgcgccct ggccctggca cgatggatcc atgatccgcg
     5101 ccgtcgtctt cgatctggac ggcgtgctgc gccgtttcga tccggctcat gtggcggcga
     5161 tcgaagcacg ccatggtctg ctcgacggca gcatccatgc cgccgcgttc gctccggaga
     5221 tgctcgatcc ggtcatcacg gggcggatca gccgcgcagc ctgggtgcgg accgtggggg
     5281 agcagctcgc gcatccgcag gcggcagagg aatggggtcg tcatccatct gtgcgggatg
     5341 ccggaatgct cgcgctcgtc gacgaactgc gttcacgcgg catccgcacc gccatcctca
     5401 cgaacggcac agacacgatc cgagccgaac ttcgcgagtc cggtatcgac gtccacgtcg
     5461 acgagatctt caactcggcc gagatcggcg tcgccaaacc cgaccctcgc gcgttccagc
     5521 atgtgctgga cgcgctggac ctgcccgcct ccgaggtctt cttcactgac gactcgtcgt
     5581 ccaagctcac cggtgcagag gcactcggca tccacacgca ccacttcacc ggcgaagtcg
     5641 cccttcgcgc cgcactctgc gacgccggca tcctcgtcag tcgctgagca ccgcgacggc
     5701 gaagccaggc acgtcgctga ccacaagatc ggctgccgta tccgtgcggg ggcgatcggt
     5761 gatccacgcc gtgcgcaacc ccgcccggcg gcccgcttcg acgtccgtct cgagcccgtc
     5821 gcccaccatc cagccgcgca gcggcacgcc gacgcgctcg gccagcgcga tcagcaggcc
     5881 cgggtcgggc ttgcggatgc cgacctccga cgacacgacc cagccgtcca ccagcacgtc
     5941 caggcccgtc cgctcgatct tcccgacctg gttgtcggcg atcccgttcg tcgcgatgcc
     6001 cagcatccat ccggctgcac gcagatcgcg cagcgcgccg accacgccgg ggaagagctc
     6061 gacgagctcg ggcatccgac ggcggtactc gtgccacagg gcggacgggt ccaccctcgc
     6121 cccgctgtgc tcgacgacac gcgggaacac ctgatccttc ggcgtgcgtg cggtcagttc
     6181 gccggtgatc caagtccgca gggcagggtc cgccccgtac gtccggatga actcgtccgc
     6241 ccagcgtccg gctgccgcct cctgatcgac gagcgtgccg tcgaggtcga agatgatcag
     6301 cccggcgctg ctcacccgat cacgtcctga tcgttcagca cccgcgagac ggtgctgcgg
     6361 cccagctccg ccatcacggg gttggaggcc tcgtagtacc agaccagtcc catggcctgc
     6421 tgcagcgccc acgcggcacc ccgccgccac tcggcatccg acgcgccgac gacctcgcgc
     6481 aggtgctggc gcggtccctc ggagagcaga tgccaggcgc acaccagatc cagcgaccgg
     6541 tccgccgggc cgaagtcgcc gccgtccagc accccggcga gcctgccgtg cgacatgagc
     6601 aggttcagcg gcgtcaagtc gcggtggctc atcacgtgct cgccggcctg aggaacctcc
     6661 cgcagctccg cccatgcccg cgagacgccg gacacgtcga cgagcccctc gctctgcgtg
     6721 atgcagtgcg cgatccactc gtcatgatcc tgcgtcgagc cgccgcgtcc gcggccgtcg
     6781 aagacccggg cgccgacagg caccgcccgc agcgcgagga tcagcgc
//
LOCUS       DATMSX010000281         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156114,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000281 DATMSX010000000
VERSION     DATMSX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156114"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VN041_08580"
     CDS             complement(<1..996)
                     /locus_tag="VN041_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWU29134.1"
                     /translation="MTGYRIGIDVGGTNTDAVILDEDLAVLASVKRPTTADTGEGVAD
                     AIDAVLAASGVDPARISHAMLGTTHCTNAIVERRGLGKVGILRLGAPATTAVPPLEGW
                     PDDLSRAVGEHVHLLAGGFEVDGRTLSALDEDAVRAACDSMREQVDAVAVVGVFSPVD
                     ESQEKRVAEIVASELGVPVSRSARIGSLGLLERENATVLNAALMQTLKQMATGFVAAL
                     RDRGIPATPYFGQNDGTLMQLEYALEFPVLTIGCGPTNSIRGAAHLSGATDALVVDIG
                     GTTTDIGVLVEGFPRQSAHAVEIGGIRTNFRMPDVLSVGLGGGTIVRSGGASLGPD"
     gene            complement(993..2111)
                     /locus_tag="VN041_08585"
     CDS             complement(993..2111)
                     /locus_tag="VN041_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWU29135.1"
                     /translation="MTALITEADIDEIALGAAVLGTGGGGDPHVGSLIAKRLIRMHGP
                     VRMLTVDDVTDEDFVVPVGGIGAPSVSVERIMAESELLAALEAIERGTGRRPTAVMPI
                     EVGGGNSMIPIAAAAVAGIPVVDGDAMGRAFPEAQMVTYHLAGYGPGTTVLVDHHGNE
                     VVSRPVNGEWSERLARAVTVEMGGGATMIDYAYGGAVARECAIPGTLTLAKRIGGILR
                     GRDAAGRQLRDDERIDALCTDLGAIRLFDGKVRDLQRAVDGGFTRGAAELDGVGADRG
                     SAFRLDFQNEMLLARRDGALAAVTPDLIAVLDLATGMPMTTDAVRYGARVAIIAIPCH
                     EKWRTPRGLETAGPAHFGYDVEWTPVEQLAAAGTEIAR"
     gene            complement(2108..3436)
                     /locus_tag="VN041_08590"
     CDS             complement(2108..3436)
                     /locus_tag="VN041_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWU29136.1"
                     /translation="METTDYEHGVVPQDQRKGWLSIAAVWIAIGIDLSGAFLGIELAS
                     GMAFWDAIWATMLGSLLLGLLAMACTYVGASTGLSTAMISRAVFGRIGGAVLAIAITI
                     SLVGWFAVQTGFFGQNAQIAFHEFTGIDVPVQLFNAVGGVLMAITAFWGYRSINRLSS
                     LAVPLLLLLLVVGVVVAFIQHGTAGLDARVDVTYTFGGAVSLVMGIFILGVVLSPDMA
                     RWAKTPKQAMAAGFVGFFFGNSIILVVAILLARVLNQTELMTMYFTLGMGGIAVVVLI
                     LAQWTTNTTNLYSAGLAFSSISSRLGRRTVTAVLAVIGIVIGVVGAADVFVSFILVIG
                     TLIAPYGGVYLASFLTGRRTARWAHGAAVPHVDAWSIIAWATGVLVAFATTNPADGPG
                     FGWFTITTISSLDGLLVGFAVYVLLWLVRGGRRADATEAVPDARTDEVAA"
     gene            complement(3488..>4041)
                     /locus_tag="VN041_08595"
     CDS             complement(3488..>4041)
                     /locus_tag="VN041_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWU29137.1"
                     /translation="AAEAEGCDAIAVDCMLDPGVHAAREVVGIPVLGPCEASMHLAAT
                     LGHRFSVISVAEHHRDLMIDQARRYGVEPRLASVRSLQIPVLELEDDPDATFRTMVAR
                     AREAVQHDHAQAIVLGCTGLAGQAAQVQSALEAEGIGVPVIDPSAAATRMLETIVAMG
                     LAGSRVTYPRRSGATSAWPEPRH"
BASE COUNT          620 a         1406 c         1455 g          560 t
ORIGIN      
        1 gtcgggcccg agggaggcgc cgccggagcg cacgatcgtg ccgccgccga gcccgaccga
       61 gagcacgtcc ggcatgcgga agttggtgcg gatgccgccg atctccacgg cgtgcgcgga
      121 ctggcggggg aagccctcca cgagcacccc gatgtcggtg gtggtgccgc cgatgtcgac
      181 cacgagagca tccgtggcgc cggacaggtg cgccgcgccg cggatcgagt tggtcggacc
      241 gcagccgatc gtgagcaccg ggaactccag cgcgtactcc aactgcatca gcgtgccgtc
      301 gttctgcccg aagtacggcg tggccgggat gccgcggtcg cgcagtgctg ccacgaagcc
      361 ggtggccatc tgcttcagcg tctgcatgag cgccgcgttc agcacggtcg cgttctcgcg
      421 ctcgagcagc ccgagtgagc cgatccgcgc ggatcgggac acagggactc cgagttcgga
      481 tgcgacgatc tcggcgacgc gcttctcctg gctctcgtcg accggcgaga acacgccgac
      541 caccgcgacc gcgtcgacct gctcgcgcat gctgtcgcat gccgctcgga cggcgtcctc
      601 gtccagtgcg gacagcgtgc ggccgtccac ctcgaaacct ccggccagca ggtgcacgtg
      661 ctcaccgacg gcgcggctga ggtcgtccgg ccagccctcc agcggcggca cggcggtggt
      721 cgcgggtgcg ccgaggcgca ggatgccgac cttcccgagt ccgcgccgct cgacgatcgc
      781 gttggtgcag tgcgtggtgc cgagcatcgc gtgcgagatc cgcgcggggt cgactccaga
      841 tgctgcgagc acggcgtcga tggcatccgc gacgccctcg ccggtgtcgg ccgtggtcgg
      901 ccgcttgacg gatgccagca cggcgaggtc ctcgtcgagg atcacggcgt cggtgttggt
      961 ccccccgacg tcgattccga tccggtaccc ggtcatcgtg cgatctccgt tcctgcggcg
     1021 gcgagctgct ccaccggcgt ccactccacg tcatagccga agtgcgcggg ccccgcggtc
     1081 tcgagcccgc gcggcgtgcg ccacttctca tggcagggga tcgcgatgat cgcgacacgg
     1141 gcgccgtagc gcaccgcatc cgtcgtcatc ggcatcccgg tggcgagatc cagcacggcg
     1201 atgaggtcgg gcgtcacggc cgcgagcgcg ccgtcgcggc gggccagcag catctcgttc
     1261 tggaagtcca gccggaacgc gctgccgcgg tcggcgccca cgccgtcgag ctcagcggca
     1321 ccgcgggtga agcccccgtc gacggcgcgc tgcaggtcgc gcaccttgcc gtcgaagagc
     1381 cggatcgccc cgagatcggt gcacaacgcg tcgatccgct cgtcgtcacg cagctgacgc
     1441 cccgccgcat ctcggccgcg caggatcccg ccgatgcgct tcgcgagtgt gagggttccg
     1501 gggatcgcgc actcccgtgc gactgcgccg ccgtacgcgt agtcgatcat ggtggcaccg
     1561 ccgcccatct cgacggtgac ggcgcgggcg agccgctccg accactcccc gttcacgggg
     1621 cggctgacga cctcgttgcc gtgatggtcg acgagcaccg tggtgcccgg gccgtacccg
     1681 gcgagatggt aggtgaccat ctgcgcctcc gggaacgcgc gacccatcgc atcgccgtcg
     1741 acgacgggga tgcccgctac ggccgctgcg gcgatgggga tcatcgagtt cccgccgccg
     1801 acctcgatcg gcatcaccgc ggtgggcctg cgcccggtac cgcgctcgat cgcctccagc
     1861 gcggcgagca gctccgactc ggccatgatc cgctcgacgc tcaccgacgg tgcgccgatg
     1921 ccgccgaccg gcacgacgaa gtcctcgtcc gtcacgtcgt cgaccgtcag catccggacc
     1981 gggccgtgca tgcggatgag gcgcttcgcg atcagcgacc cgacatgcgg gtcgcctccg
     2041 ccgccggtgc cgagcaccgc cgcgccgagc gcgatctcgt cgatgtcggc ctcggtgatc
     2101 agcgcggtca tgcggccacc tcgtccgtcc gggcatccgg caccgcttcc gtggcatccg
     2161 ctcgccgtcc accgcggacc agccacagca gcacgtacac ggcgaagccg acgagcaggc
     2221 cgtccagtga cgagatcgtc gtgatcgtga accagccgaa accgggtccg tccgcggggt
     2281 tcgtggtcgc gaaggcgacc agcacgccgg tggcccaggc gatgatcgac catgcatcca
     2341 cgtgcggcac ggcggcgccg tgcgcccacc gcgcggtgcg acgtccggtc aggaacgacg
     2401 ccaggtagac gccgccgtag ggcgcgatga gcgtgccgat caccaggatg aacgagacga
     2461 acacgtccgc cgcaccgacg acaccgatca cgatgccgat cacagcgagc acggcggtca
     2521 cggtgcggcg cccgagcctg gacgagatcg acgagaacgc gaggccggcc gagtagaggt
     2581 tcgtggtgtt cgtggtccac tgcgcgagga tcagcacgac gacggcgatg ccgcccatcc
     2641 cgagcgtgaa gtacatcgtc atcagttcgg tctggttcag cacgcgggcc agcaggatcg
     2701 cgacgacgag gatgatcgag ttgccgaaga agaacccgac gaagccggcc gccatggcct
     2761 gcttcggcgt cttcgcccag cgggccatat cgggagagag cacgacgccg aggatgaaga
     2821 tgcccatgac cagcgacacc gcgccgccga acgtgtaggt gacgtcgacc cgggcgtcca
     2881 gccccgcggt tccgtgctgg atgaaggcga ccaccactcc gacgaccagc agcaggagca
     2941 gcagcggcac cgcgagcgag ctcaggcggt tgatcgagcg gtagccccag aacgcggtga
     3001 tcgccatcag cacgccgccg acggcgttga agagctgcac gggcacgtcg atgccggtga
     3061 actcgtggaa ggcgatctgg gcgttctgtc cgaagaagcc ggtctgcacg gcgaaccagc
     3121 cgaccaggct gatcgtgatg gcgatggcca gcaccgcgcc cccgatccgg ccgaacacgg
     3181 cgcgcgagat catcgcggtg gacaggccgg tcgaggcgcc gacgtaggtg caggccatgg
     3241 ccagcagtcc gagcagcagc gagccgagca tggtggccca gatggcgtcc cagaacgcca
     3301 tgccggaggc gagttcgatg ccgaggaacg cgccggagag atcgattccg atcgcgatcc
     3361 agacggcggc gatcgaaagc cagcccttgc gctgatcctg gggcacgacg ccgtgctcgt
     3421 agtcggtggt ttccatgagg ggtccttgct tggtgagggg tgaggggctt ggtgacgggt
     3481 gaggggctca gtgacggggt tcgggccagg cgctcgtggc gccggaacgt cgggggtagg
     3541 tgacgcggct gccggcgagc cccatcgcga cgatggtctc gagcatccgg gtggcggcgg
     3601 ccgaggggtc gatgacgggc accccgatcc cctcggcctc gagcgccgac tgcacctggg
     3661 cggcctggcc ggccagcccg gtgcatccga gcacgatcgc ctgggcatgg tcgtgctgca
     3721 cggcctcgcg ggcacgggcc accatcgtgc ggaaggtcgc gtcggggtcg tcctcgagtt
     3781 cgaggacggg gatctgcagc gaccggacgg atgccagccg cggctcgaca ccgtaccggc
     3841 gggcctggtc gatcatcagg tcgcggtggt gctcggcgac ggagatcacc gagaagcggt
     3901 ggccgagggt ggcggccagg tgcatggatg cctcgcacgg gccgagcacg gggatcccga
     3961 cgacctcgcg cgccgcgtgc actccggggt cgagcatgca gtccacggcg atcgcgtcgc
     4021 acccctcggc ctcggccgcc t
//
LOCUS       DATMSX010000282         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_118055,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000282 DATMSX010000000
VERSION     DATMSX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118055"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VN041_08600"
     CDS             <1..751
                     /locus_tag="VN041_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWU29138.1"
                     /translation="VKLAEAHAANPCADAAVTSALAAHDDAAAVRAFGGGEQFRSAVT
                     AGNAPCISLSDPAHDWVVVNKLRPLAPVDFAPASVRASSLRATSGSVSMRPEVADALD
                     RMSADLTAGGAGTIGVNNAYRSYGLQQVTYSRFVRSEGVAGADAGSARPGHSEHQSGL
                     AVDVVACSEGCGSLGAFGSTAQSNWIVQHAWEYGFIVRYEKGATPTTGYMPEPWHLRF
                     IGVELAKAYHDGGFHTLEEFFGLPAAPDYAH"
     gene            904..2067
                     /locus_tag="VN041_08605"
     CDS             904..2067
                     /locus_tag="VN041_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWU29139.1"
                     /translation="MERDIYEEDHEAFRDLVKDFVKRYATNENIEKWEAAGEIDRATM
                     LAAGEAGLIGLSVPEEFGGAGMLQDYRFRTIVMEEVIAAGAGSLAGAFGIQDDLAVPY
                     LVHMGTQEQKEKWLPRMATGEVLGALAMTDPGAGSDLRGIKTNAKKVDGGYILNGAKT
                     FISSGTTADIVVTFVKTGEGNRPDAFSLLIVEKGMEGFDQGKKLSKMGFHGWDTAELS
                     FTDVFVPDENLISGKEGQGFIQLMMNLPLERLSIGVAAAAAAEAATNWTIAYTKDREA
                     FGERIADFQNTRFRLADMVTTTEVMWAYVDRALLAYKDSKLSAEDAAKVKFWATEREW
                     EVLDMGVQLHGGYGYIMEYPIAKAFTDARVHRIYGGTNEIMRDLVGRQIAGKR"
     gene            complement(2246..3121)
                     /locus_tag="VN041_08610"
     CDS             complement(2246..3121)
                     /locus_tag="VN041_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HWU29140.1"
                     /translation="MSVGKIVKPASAELIHLTVLRTQRLTSGWMRVTLGGGEIERFTP
                     MGYDQWFRLFLPLGGEEGLERVPAKANGLLGYLKFLRIPDGVRPVMRNYTVRSYRPAS
                     ADAGAEIDVDFVLHGSAADGTAGPASRWAETCRPGEHVLVIDEGLTFNPARGEDRVVL
                     VGDETALPAIAGICSSLPTTAVGTVIVEVAAAEDALEFAHPAAIEVQTIVRTDAEVPG
                     VRALSALRELALPSMPFHAYIAGEQSLASGARRHLVAELGVDKDHVSFTGYWKRGASS
                     PASRASREAAARPAN"
     gene            complement(3143..>3343)
                     /locus_tag="VN041_08615"
     CDS             complement(3143..>3343)
                     /locus_tag="VN041_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWU29141.1"
                     /translation="VVPLLASGIRHAERVALSMDARAFGAHRTRTERHLVPWRTRDTV
                     FVGAVVLASAVVFTATFPWQLP"
BASE COUNT          552 a         1160 c         1145 g          486 t
ORIGIN      
        1 cgtgaagctg gccgaggcgc acgccgcgaa tccgtgcgcg gatgctgctg tgacgtccgc
       61 gctcgccgcg cacgacgatg ccgccgctgt gcgcgccttc ggtggtggcg agcagttccg
      121 ttccgcggtc acggcgggca acgccccgtg catctcgctc tccgatcccg ctcatgactg
      181 ggtcgtcgtc aacaagctcc gcccgctggc cccggtggac ttcgcgcctg cgtccgtgcg
      241 ggcgtcgagc ctgcgtgcca cctccggttc ggtgagcatg cgccccgagg tcgcggacgc
      301 cctcgaccgg atgagcgccg acctgacggc cggaggagcc ggcacgatag gcgtcaacaa
      361 cgcctatcgc tcctacggcc tgcagcaggt cacatactcc cgcttcgtgc gctcggaggg
      421 tgtcgcggga gcggatgccg gatccgcgcg ccccggtcac agcgagcacc agagcggcct
      481 ggccgtcgac gtcgtcgcct gctcggaggg ctgcgggtcg ctcggcgcgt tcggttccac
      541 cgcgcagagc aactggatcg tgcagcacgc ctgggagtac gggttcatcg tccgctatga
      601 gaagggcgcc acgccgacca ccggctacat gccggagccg tggcatctgc gcttcatcgg
      661 cgtggagctg gcgaaggcat accacgacgg cggcttccac acgctggagg agttcttcgg
      721 gctccccgca gcccccgatt acgcgcactg agggcctccg gaaacgccca ctgagggcat
      781 cccacaaacc cggaacgcag tgccacggat tgtgggacgc attcccacaa tccgctgcgt
      841 ccggacctcg tcgcgtcgta gactcgccgg tgcgaagacg cgcacgactt tcaggaggac
      901 gtcatggaac gcgacatcta cgaagaggat cacgaggcct tccgcgacct ggtcaaggac
      961 ttcgtcaagc ggtacgccac gaacgagaac atcgagaagt gggaggccgc cggcgagatc
     1021 gaccgcgcca ccatgctcgc cgccggtgag gcgggactga tcggcctttc ggtgcccgag
     1081 gagttcggcg gcgccggaat gctgcaggac taccgcttcc gcacgatcgt catggaggag
     1141 gtcatcgccg cgggtgcggg ctcgctggcc ggtgccttcg gcatccagga cgacctggcc
     1201 gtgccgtacc tggtgcacat gggcacccag gagcagaagg agaagtggct gccccgcatg
     1261 gccaccggtg aggtgctcgg cgcgctcgcc atgaccgacc cgggtgccgg ctccgacctg
     1321 cgcggcatca agaccaacgc caagaaggtc gacggcggct acatcctcaa cggcgccaag
     1381 acgttcatct cctccggcac gaccgccgac atcgtggtca ccttcgtcaa gaccggtgag
     1441 ggcaaccgcc ccgacgcgtt cagcctgctg atcgtggaga agggcatgga gggcttcgac
     1501 cagggcaaga agctcagcaa gatgggcttc cacggctggg acacggcgga gctcagcttc
     1561 actgacgtct tcgtgcccga cgagaacctc atcagcggca aggagggtca gggcttcatc
     1621 cagctgatga tgaacctgcc gctcgagcgc ctctcgatcg gtgtcgccgc cgctgcggcc
     1681 gctgaggccg cgaccaactg gaccatcgcg tacaccaagg accgcgaggc cttcggcgag
     1741 cgcatcgccg acttccagaa cacccgcttc cgcctggccg acatggtcac caccaccgag
     1801 gtgatgtggg cgtacgtcga ccgcgcgctg ctggcctaca aggacagcaa gctcagcgcc
     1861 gaggacgccg ccaaggtgaa gttctgggcc acggagcgcg agtgggaggt gctcgacatg
     1921 ggcgtgcagc tgcacggcgg ctacggctac atcatggagt acccgatcgc gaaggcgttc
     1981 accgacgccc gcgtgcaccg catctacggc ggcaccaacg agatcatgcg cgacctcgtc
     2041 ggccgtcaga tcgccggcaa gcgctgacgc atcaccatcg aggggccgtc cggatgctgc
     2101 atccggacgg ccctcgtcgt cccgctcgac ctgtcctgac agtacagatg tcggtcggga
     2161 gtacggatgt cggccggacg cccccgtttc ggccgacatc cgtactctga gccgacatcc
     2221 gtaccggtcg aagggcggag cgggctcagt tcgcaggccg ggccgccgcc tcgcgggacg
     2281 cgcgggatgc gggcgacgag gcgccgcgct tccagtagcc ggtgaagctc acatggtcct
     2341 tgtccacgcc gagctcggcg acgaggtgcc gccgggcgcc ggaggccagg gactgctcgc
     2401 ccgcgatgta tgcgtggaac ggcatggacg gcagcgccag ctcgcgcagc gcggacagcg
     2461 cgcgcacgcc gggcacctca gcatccgtgc gcacgatcgt ctgcacctcg atggctgccg
     2521 gatgcgcgaa ctcgagcgcg tcctccgcgg ccgcgacctc gacgatcacg gtcccgaccg
     2581 ccgtcgtcgg cagcgaggag cagatgcccg cgatcgcggg cagagccgtc tcgtcgccca
     2641 ccagcacgac gcggtcctct ccgcgtgcgg ggttgaacgt cagtccctcg tcgatcacca
     2701 ggacgtgttc gccaggacgg caggtctccg cccatcgcga tgcgggccct gctgtgccgt
     2761 cagccgccga cccgtgcagc acgaagtcca cgtcgatctc ggcgccggca tcggcgctcg
     2821 ccgggcggta cgaccgcacg gtgtagttgc gcatcaccgg acgcacgccg tcggggatgc
     2881 gcaggaactt caggtacccg agcaggccgt tggccttcgc gggcacgcgc tccagtccct
     2941 cctcgccgcc cagcggcagg aacagccgga accactggtc gtatcccatc ggggtgaagc
     3001 gctcgatctc accgccgccg agcgtcaccc gcatccagcc actggtgagt cgctgcgtgc
     3061 gcagcacggt caggtggatg agctccgcgg atgcgggctt gacgatcttg ccgacggaca
     3121 tgcggaacct cagttcattc gatcagggga gctgccaggg gaaggtggcg gtgaacacga
     3181 cggcggatgc caggaccacg gcgcccacga acaccgtgtc gcgcgtgcgc cacgggacga
     3241 ggtgccgctc ggtgcgggtg cggtgggcgc cgaaggcgcg ggcatccatc gacagcgcga
     3301 cgcgttcggc atgccggatg ccggatgcca gcagcggcac gac
//
LOCUS       DATMSX010000283         8592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_146348,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000283 DATMSX010000000
VERSION     DATMSX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146348"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VN041_08620"
     CDS             <1..308
                     /locus_tag="VN041_08620"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWU29142.1"
                     /translation="SRAWIRPQHPLLDGEAISSRSRMLGMADFANGIATRVAPERALY
                     PNIDLTASIFREPTGDWFGLDTSVSFGPDGIGLTESVLSDADGPVGTSSQTLTVRPR"
     gene            complement(346..1788)
                     /locus_tag="VN041_08625"
     CDS             complement(346..1788)
                     /locus_tag="VN041_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29143.1"
                     /translation="MATSGLELSTLGHRIRHHRTKKGFTLDELGALVGIAGSQLSLIE
                     NGKREPKLSLLQAIALACSTDVADLISGEPPNHRAALEIELDRAQTSPVFRQLGIAPI
                     RVSKSMSDETIEAVLGLHRELHRREREAIATPEEARRANTELRLTMREKSNYLPEIEA
                     LAEKQLKAAGHVSGALTHRTVSIMAERLGFELIYVDDLPHSTRSITDLDNGRIYLPPA
                     SIPGGHGLRSMALQAMAHRLLGHTRPTDYADFLQQRLEINYFAACCLIPESAGVAFLQ
                     QARKDRNLAVEDFRDAFGVTHEAAGLRMTNLMTHHLDMRLHFLRVDENGAITRVYEND
                     DLPLPMDVTGAVEGQPACRKFSARAAFEQRNRTTEHYQYTDTPSGTFWCATQTGSTSS
                     GGFSITVGVPFDDARWWRGRETTHRAQSTCPDEACCRRAPLAVSQRWEGRAWPSARVH
                     THMFSPLPRGAFPGVEESEVYAFLDRHVDE"
     gene            1991..3772
                     /locus_tag="VN041_08630"
     CDS             1991..3772
                     /locus_tag="VN041_08630"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWU29144.1"
                     /translation="MAELREWVDGIAALTQPDRVHWIDGSRAENDAILRELVDEGKII
                     KLNPEWRPGSYLARSHPSDVARTEARTFISSEKEIDAGPTNNWADPVEMRDTMNRIFA
                     GSMRGRTMYVIPFSMGPIGGKLSHIGVQVTDSAYAVASINIMTRVGDAVTRQIAEGAP
                     WVKTVHSVGAPLEPGQADVEWPCNDEKYIVHFPDTLEVYSYGSGYGGNAILAKKCFAL
                     RIASVIARDEGWLAEHMLLIRVIDPKGKAYHVAAAFPSACGKTNLAMLRPTIPGWKVE
                     TLGDDIVWIRPGEDGRMYAMNPEAGFFGVAPGTGESTNVTAVETLWGNTIFTNVALRP
                     DGDVWWEGLTDEAPSELTDWQGNPWTPESGRPAAHPNSRFTVSAAQCPQISADWEDPE
                     GVALDVILFGGRRATNVPLVVEATDWTHGVFLGSTISSERTAAAEGTVGELRRDPFAM
                     LPFCGYNMGDYFSHWLKVGRQLRFDRAPRIFQVNWFRRGEDGRFLWPGFGDNSRVIDW
                     IIRRISGEVGAVDSPIGRLPRREDLNLDGLTISDADLDELFDVDVDAWMAEADSTGEY
                     FGIFGDRLPAALRSELEALRYRLSAAR"
     gene            3947..5125
                     /locus_tag="VN041_08635"
     CDS             3947..5125
                     /locus_tag="VN041_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain"
                     /protein_id="HWU29145.1"
                     /translation="MRTLIVGAGVAGPALAYWLRRAGHEVTLVERAPRLRTGGYLIDF
                     WGAGFEVADRMGIAPRLIEEGYVLSGAYDVDDRGRVRARVNQARLAESVGGRYVSIVR
                     SDLARALCDCVDAGVEFILGDTVAALTDQGDHVDVEFERSEPRRFDLVFGADGLHSRV
                     RELAFGPQDRFEQELGIAVAAFDVDGYRPRDENTVVMHAAVGSQSARVALRDDVTLFL
                     LTFRHDGALPDDPEAQRELVRTAMADAGGETPKILEHLPRARTLYLDRASQIRMPQWS
                     RGRIALLGDAAACPSLLAGQGAALAMAEAYVLAVELVRSGGDHRVAFREYERRMMPMV
                     LAKQDGARGLATAFAPLTRGKLRLRNALFRMMGIRFVANLVMGRSLRDPIEVPDWPGV
                     "
     gene            5127..5744
                     /locus_tag="VN041_08640"
     CDS             5127..5744
                     /locus_tag="VN041_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU29146.1"
                     /translation="MPRVRFERLDSAQQAVILDAALREFSTDGYADASLNRIIAAAGL
                     SKGAMYYYFEDKADLYLYVVSTQMARLAGSPADLEFLRARSADDFWSMLESDSIRVLT
                     RLSELPDVAAVLRDAIAGTGAAVMRRGGGEVEAVTQSWIERAIGYGQQRGAVRADVPI
                     GLLGAVAGAMMNAMDAWYLSRRDVAQPDRRVVHDFIGMLRRALQP"
     gene            complement(5762..7639)
                     /locus_tag="VN041_08645"
     CDS             complement(5762..7639)
                     /locus_tag="VN041_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29147.1"
                     /translation="MSRTATARRRGRGAQQDGPRATFRQLLPFLFEHKRVLVAVALLS
                     IVGAAASLAQPLLVGQVIDRVQKGEMLGALVWLLIGLVIVASVISGYQHYLLQRTGTA
                     VVYSSRRKLIARILHLPISEFDARRTGDLVSRVGTDTTLLYAVLTQGLADAVGGAILF
                     IGALIAMLLIDPLLLLLIVLVIGASVVVVVLLSGRIRIASAAQQHKVGELASGIERGI
                     SSIRTVRASGATERETAAVDGIAGEAYGLGVRIAKISSMVVPVAGIALQLSLLVVLGV
                     GGFRVASGALSIASLVTFIMFLFMLVMPLGQAFGAITSVNQALGALGRIQEILDLPTE
                     TQDDESDAAITAAAVAGAPAIEFRDVRFRYPDDVIAAREAAAKEARNLLADAHLRRAG
                     EADPEISRDVLKGISFAVPRGARVALVGPSGAGKSTTLALIERFYDPTGGSIRLAGHD
                     VRSYPRQQLRAHFGYVEQDAPTLAGTLAENLRLAAPDATDADCERVLRSVNLGDVLER
                     SPLGLDAPVGEDGVMLSGGERQRLAIARALLTEAPILLLDESTSSLDGVNEQRMREAI
                     DAAAADRTLVVIAHRLSTVVDSDLIVVLQDGLVVGQGTHDELIESTPLYRDLARHQLL
                     V"
     gene            complement(7693..7899)
                     /locus_tag="VN041_08650"
     CDS             complement(7693..7899)
                     /locus_tag="VN041_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HWU29148.1"
                     /translation="MTPHTALAVDPEGEPIAELHRLTGIRRELARAEEAQVRRARLAG
                     YSWQAIASALGVTKQAAHKKFGRQ"
     gene            complement(7987..8400)
                     /locus_tag="VN041_08655"
     CDS             complement(7987..8400)
                     /locus_tag="VN041_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29149.1"
                     /translation="MATIEDVRRIAMSLPRVVERSGGHNGGLAWRTSAGMLVWERPPR
                     RHDLEQLTELGREWPEGVVVGIRVDGEQAKAAAIETYPEVFFTIPHFDGFPAVLTRLD
                     AIDEQLLREMLTDAWLLRVPKTVATAWLAENGLSE"
BASE COUNT         1282 a         2940 c         3024 g         1346 t
ORIGIN      
        1 ggtcgcgcgc gtggatccgc ccgcagcatc cgctcctgga cggggaggcg atctcgtcac
       61 ggtcgcggat gctgggcatg gccgacttcg ccaacggcat cgccacgcgg gtcgcgcccg
      121 agcgcgcgct ctacccgaac atcgacctga ccgcgagcat cttccgcgag cccacgggcg
      181 actggttcgg cctggacacc tcggtgtcgt tcggacccga cggcatcggg ctcaccgagt
      241 ccgtgctcag cgacgccgat ggcccggtgg gcacctcgtc gcagacgctg accgtgcgcc
      301 cgcgctgagt cgcggccggc gatccggccg ggactcagcg ttctgctact cgtcgacgtg
      361 gcggtcgagg aacgcataga cctcgctctc ctcgacgccg gggaaggcgc cgcgggggag
      421 cggcgagaac atgtgcgtgt gcacccgagc gctcggccac gcgcgcccct cccaccgctg
      481 cgacaccgca aggggcgcgc gtcggcagca cgcctcgtca gggcaggtcg actgcgcacg
      541 atgggtggtc tcgcgtccgc gccaccagcg cgcatcgtcg aacggcacgc cgaccgtgat
      601 cgagaagccg ccggagctgg tcgacccggt ctgggtcgcg caccagaacg tgcctgacgg
      661 ggtgtcggtg tactggtagt gctcggtggt gcggtttcgc tgctcgaacg cggcccgtgc
      721 ggagaacttg cggcaggcgg gctgcccctc gaccgccccc gtgacatcca tcggcagggg
      781 aagatcgtcg ttctcgtaca cgcgggtgat cgcgccgttc tcgtccacgc gcaggaagtg
      841 caggcgcatg tcgaggtggt gggtcatcag gttggtcatg cgcaggcccg cggcctcgtg
      901 cgtgacgccg aaggcgtcgc ggaagtcctc gaccgcgagg ttgcggtcct tcctggcctg
      961 ctgcaggaag gccactccgg cgctctccgg gatcaggcag cacgcggcga agtagttgat
     1021 ctccagccgc tgctgcagga agtcggcgta gtcggtcgga cgggtgtgcc cgagcagtcg
     1081 atgcgccatg gcctgaagcg ccatggaacg caggccgtgc ccgccgggga tcgaggccgg
     1141 cggcaggtag atgcggccgt tgtccaggtc ggtgatggag cgcgtggaat gcggcaggtc
     1201 gtcgacgtag atcagctcga agccgagtcg ctcggccatg atgctgaccg tgcggtgcgt
     1261 cagcgcgccg gacacgtggc cggcggcctt cagctgcttc tcggcgagcg cctcgatctc
     1321 cggcaggtag ttcgacttct cgcgcatggt gaggcgcagc tcggtgttgg ctcggcgcgc
     1381 ctcctccggc gtggcgatgg cctcgcgctc gcggcggtgc agttcgcggt gcaggcccag
     1441 cacggcctcg atggtctcgt cgctcatgct cttcgacacc cggatgggcg cgatgcccag
     1501 ctggcggaac accgggctcg tctgtgcgcg atccagctcg atctccagcg ccgcgcggtg
     1561 gttcggcggc tcacccgaga tgaggtcggc gacgtcggtg ctgcacgcca gggcgatggc
     1621 ctgcagcagc gagagcttgg gctcgcgctt gccgttctcg atcaggctca actgcgaacc
     1681 ggcgatcccc accagggcgc cgagctcgtc gagcgtgaag cccttcttgg tgcggtgatg
     1741 ccggatgcga tgtccgaggg tgctgagctc gagtccactt gtcgccattc cttgagcata
     1801 cccgaaagaa acatgcttct ttcaagctga aacggccgaa agcgtgcgga aagtacgcgg
     1861 atgatggatg tatcgccccc ctcttcacaa aggagcactc atggccatgg ctgaaacata
     1921 cgcccctcgc acctccccgg tcgcccccgt gcgcagcttc ggggccgtgc cggcgtatga
     1981 caccccggcc atggccgagc tgcgcgaatg ggtcgacggc atcgccgcgc tcacccagcc
     2041 cgaccgcgtc cactggatcg acggctcccg tgcggagaac gacgcgatcc tgcgggaact
     2101 ggtcgacgag ggcaagatca tcaagctcaa ccccgagtgg cgccccggct cctacctggc
     2161 ccgctcgcac cccagcgacg tcgcgcgcac cgaggcccgt accttcatct cctccgagaa
     2221 ggagatcgac gccggaccga ccaacaactg ggcggacccg gtcgagatgc gcgacaccat
     2281 gaaccgcatc ttcgccggct cgatgcgcgg ccggacgatg tacgtcatcc ccttctcgat
     2341 gggccccatc ggcggcaagc tctcgcacat cggcgtccag gtcaccgaca gcgcctacgc
     2401 cgtggcatcc atcaacatca tgacccgcgt cggcgacgcc gtcacccgcc agatcgccga
     2461 gggcgccccg tgggtcaaga ccgtgcactc ggtcggagcc ccgctcgagc ccggtcaggc
     2521 cgacgtcgag tggccctgca acgacgagaa gtacatcgtg cacttccccg acacgctcga
     2581 ggtctactcc tacggctccg gctatggcgg gaacgccatc ctcgccaaga agtgcttcgc
     2641 gctgcgcatc gcgtcggtga tcgctcgcga cgagggctgg ctcgccgagc acatgctgct
     2701 catccgcgtg atcgacccga agggcaaggc gtaccacgtg gccgccgcgt tcccctcggc
     2761 ctgcggcaag accaacctcg ccatgctgcg cccgacgatc cccggctgga aggtcgagac
     2821 cctcggcgac gacatcgtct ggatccgtcc cggcgaggac ggccgcatgt acgcgatgaa
     2881 ccccgaggcc ggcttcttcg gcgtcgcccc cggcaccggc gagtcgacca acgtcaccgc
     2941 cgtcgagacc ctctggggca acacgatctt cacgaacgtg gcgctgcgcc cggacggcga
     3001 cgtgtggtgg gagggcctga ccgatgaggc gccgtccgag ctgaccgact ggcagggcaa
     3061 cccgtggacg ccggagtccg gccgccccgc ggcgcacccg aactcccgct tcaccgtctc
     3121 ggccgcgcag tgcccgcaga tctctgcgga ctgggaggac cccgagggcg tcgccctgga
     3181 cgtcatcctg ttcggcggtc gccgcgcgac caacgtgccg ctcgtcgtcg aggccaccga
     3241 ctggacgcac ggcgtcttcc tcggatcgac gatctcgtcc gagcgcaccg ctgccgcaga
     3301 gggcacggtc ggcgagctgc gtcgcgaccc gttcgcgatg ctcccgttct gcggctacaa
     3361 catgggcgac tacttcagcc actggctcaa ggtcggacgc cagctgcgct tcgatcgtgc
     3421 cccgcgcatc ttccaggtga actggttccg ccgcggcgag gacgggcgct tcctgtggcc
     3481 gggcttcggc gacaactcgc gcgtgatcga ctggatcatc cgtcgcatct cgggcgaggt
     3541 cggagccgtc gacagcccga tcggccgcct gccgcgccgt gaggacctca acctcgacgg
     3601 gctgacgatc tcggatgccg atctcgacga gctgttcgat gtcgacgtcg acgcctggat
     3661 ggccgaggcc gattcgaccg gagagtactt cggcatcttc ggcgaccggc ttcccgccgc
     3721 tctgcgcagc gagctggagg cgctgcgcta ccgcctcagc gccgcgcgct gaacgcaccc
     3781 tgacccgcga cggcgggatg ctggagacga cccatggctg agtggtctcc ggcatcccgc
     3841 cgtcgcgctt ctccgccgtc gctgctccag gattgaggct ttcggagagc tgtgggacga
     3901 ggtagtttca gaccagtggt ctaaccagtg gtctgaaagg ttgagcatgc gcacattgat
     3961 cgtcggcgcc ggtgttgccg gcccggcgct ggcgtactgg ctgcgtcgcg ccggtcacga
     4021 ggtcacgctt gtcgaacgcg cgccccggtt gcgcacgggc ggctatctga tcgacttctg
     4081 gggcgccggg ttcgaagtgg ccgacagaat gggcatcgcc ccgcgcttga tcgaggaggg
     4141 gtacgtgctc tccggtgcgt acgacgtgga tgatcgcgga cgggttcgcg cgagggtgaa
     4201 ccaggcacgt ctggcggaat cggtgggtgg ccgttacgtc agcatcgtgc gttcagatct
     4261 cgcgcgggcg ctctgcgact gcgtggatgc aggtgtcgag ttcattctgg gcgacacggt
     4321 cgcagccctg accgatcagg gtgaccacgt cgacgtcgag ttcgaacgct ccgaaccgcg
     4381 gcggttcgac ctcgtgttcg gcgcggacgg actgcattcg cgcgtgcgcg agctcgcttt
     4441 cggcccgcag gaccggttcg agcaggaact cggcatcgct gtcgcggcgt tcgacgtcga
     4501 tggctaccgc cccagggacg agaacactgt ggtcatgcac gcggcggtcg gctcgcagag
     4561 cgcgcgggtc gcgctgcgcg atgatgtcac gctgttcctg ctcacgttcc ggcacgacgg
     4621 cgccctgccg gacgatcccg aggcgcaacg agagctggtg cgaacggcga tggcggatgc
     4681 cggcggcgag acgccgaaga tcctggaaca cctgccgcgg gcacgaaccc tgtacctcga
     4741 tcgtgccagc cagatcagga tgccacagtg gagccggggc cgcatcgccc tgctcggcga
     4801 tgcggcggcc tgtccctcgc tgctcgccgg acagggagca gcgctggcaa tggccgaggc
     4861 ctacgtgctg gcggtggagc tggtgcgcag cggtggagat catcgggtcg cgttcaggga
     4921 gtacgagcga cggatgatgc cgatggtcct ggcgaagcag gacggggccc gcgggcttgc
     4981 gacagccttc gcgccgctca cccgcggcaa gctccggctt cggaatgctc tgttccggat
     5041 gatgggaatc cggttcgtgg cgaatctcgt gatggggcgc agcctgcgcg acccgatcga
     5101 ggtgccggac tggccggggg tgtgaggtgc cacgcgtccg tttcgaacgc ctcgattccg
     5161 cgcagcaggc cgtcatcctg gatgccgctc tgcgtgagtt ctccaccgac ggctatgccg
     5221 acgcatcgct gaaccgcatc atcgccgcgg cgggactgtc gaagggggcg atgtactact
     5281 acttcgagga caaggctgac ctgtacctgt acgtggtcag cactcagatg gcgcgcctgg
     5341 cggggtctcc tgcggatctc gagttcctgc gcgcccggag cgcggacgac ttctggtcga
     5401 tgctggagtc cgacagcatc cgggtgctga cccggctctc ggagcttccc gacgtcgcgg
     5461 ccgtcctgcg ggatgcgatc gcgggcacag gggctgccgt gatgcggcgc gggggaggcg
     5521 aggtcgaagc ggtgacgcag tcctggatcg agcgcgccat cgggtacggg cagcagcggg
     5581 gcgccgtacg cgccgatgtg ccgatcgggc tgctcggcgc cgtcgccggc gcgatgatga
     5641 atgcgatgga cgcctggtat ctctcccgcc gtgatgtcgc gcagccggac cgtcgcgtcg
     5701 tgcacgactt catcgggatg ctgcggcgag cactgcaacc ctgactgccg cgtcgccagg
     5761 ctcagaccag cagctgatgc ctggcgaggt cgcggtacag cggcgtggac tcgatcagct
     5821 cgtcatgcgt gccctggccg accacgaggc cgtcctgcag caccacgatc aggtcgctgt
     5881 ccaccacggt cgacaggcgg tgcgcgatca cgacgagcgt gcggtcggca gcggccgcgt
     5941 cgatggcctc gcgcatccgc tgctcgttca cgccgtccag tgaggacgtc gactcgtcca
     6001 gcagaaggat cggagcctcc gtcagcagcg cgcgggcgat cgcgagacgc tggcgctcac
     6061 ctcccgagag catcacgccg tcctcgccga caggggcgtc caggcccagc gggctccgct
     6121 cgagcacgtc gccgaggttc accgagcgca gcacgcgctc gcagtcggca tccgtggcat
     6181 ccggcgctgc caggcgcagg ttctcggcca gcgtgccggc gagcgtcggg gcatcctgct
     6241 ccacataccc gaagtgggcg cgcagctgct gccgtgggta gctgcgcacg tcgtggcccg
     6301 cgagccggat cgagccgccg gtcgggtcgt agaagcgctc gatgagcgcg agggtggtgc
     6361 tcttgcccgc accggaaggt ccgaccaggg ccactcgcgc tccgcgcggc acggcgaacg
     6421 agatgccctt cagtacgtcg cggctgatct cagggtccgc ctcgcctgcc cgccgcaggt
     6481 gcgcgtcggc gagcaggttc cgcgcctcct tcgcggccgc ctcccgcgcg gcgatgacgt
     6541 cgtcggggta gcggaagcgc acgtcgcgga attcgatggc aggggcaccc gcgaccgcgg
     6601 ccgcggtgat ggcggcatcc gattcgtcgt cctgcgtctc ggtgggcaga tcgaggatct
     6661 cctggatgcg gcccagcgcg cccagcgcct ggttcaccga ggtgatggcg ccgaacgcct
     6721 gaccgagggg catgaccagc atgaacagga acatgatgaa cgtgaccagc gaggcgatgc
     6781 tgagggcgcc ggacgccacg cggaacccgc ccacgcccag caccaccagc agcgagagct
     6841 gcagggcgat gccggcgaca ggcacgacca tcgaggagat cttcgcgatg cgcacgccca
     6901 ggccgtacgc ctcgccggcg atgccgtcga cggcggccgt ctcccgttcg gtcgcgccgg
     6961 aggcgcgcac ggtgcggatg ctggagatgc cgcgctcgat gccggaggcg agctcgccga
     7021 ccttgtgctg ctgcgcggcg gatgcgatgc ggatccggcc gctgagcagc acgacgacca
     7081 ccaccgatgc gccgatcacg agcacgatca gcagcaggag cagtgggtcg atgagcagca
     7141 tggcgatgag ggcgccgatg aagaggatgg cgccgccgac cgcatccgcc agcccctgcg
     7201 tgaggaccgc gtacagcagc gtcgtgtcgg tgcccacacg ggagacgagg tcgccggtgc
     7261 ggcgcgcgtc gaactcgctg atcggcaggt gcaggatgcg cgcgatcagc ttgcgacggc
     7321 tggagtagac cacggccgtg ccggtgcgct gcagcaggta gtgctggtag ccggagatga
     7381 ccgacgccac gatcaccagt ccgatgagca gccagaccag ggcgcccagc atctcgccct
     7441 tctgcacgcg gtcgatgacc tggccgacga gcagcggttg cgcgagggat gccgccgcgc
     7501 cgacgatgct gagcagcgcg acggccacca gcaccctctt gtgctcgaag aggaagggga
     7561 ggagctggcg gaacgtggcg cgcgggccgt cctgctgcgc gccgcgcccg cgtcggcgtg
     7621 ctgtcgccgt gcgagacatg gggaacctcg ttctggggag cggtgcgacg gtatcgggcc
     7681 gcggcatccg atctattgtc tgccgaactt cttatgcgcc gcctgtttgg tgacgccgag
     7741 ggcgctcgcg atcgcctgcc aggagtatcc ggccaggcgg gcgcggcgca cctgcgcctc
     7801 ctccgctcgg gccagctcgc gtcggatgcc ggtgagccga tgcagctcgg cgatcggctc
     7861 tccctcggga tcgacggcga gggcggtgtg cggggtcatg gagtcaatct aggttgactc
     7921 gtgtcatttg tcaatagaag ttgacggctg atcctgtgtc cgccacgggg agtcctgcgg
     7981 atccggctat tcggacagcc cgttctcggc gagccaggcg gtggcgaccg tcttcggcac
     8041 gcgcagcagc caggcatccg tgagcatctc gcgcagcagc tgctcgtcga tggcgtccag
     8101 ccgggtgagc accgcgggga agccgtcgaa gtgcgggatg gtgaagaaca cctccgggta
     8161 ggtctcgatg gccgcggcct tcgcctgctc gccgtcgacc cggatgccga cgaccacgcc
     8221 ctccggccat tcccgtccga gctccgtcag ctgctcgagg tcatgtctgc gcggcggccg
     8281 ctcccacacc agcatcccgg cgctcgtgcg ccacgcgagc ccgccgttgt ggccgccgct
     8341 gcgctccacc acgcgcggga gactcatcgc gatccgacgc acatcctcga tcgtcgccat
     8401 gccgccaggc tacgcggacc cgtgcccggc atccagggcg gggtgatccc gaccgagggg
     8461 tgagtcagcc cagccgcacg cgcccgcgca gctcctgcac ggtggagacg ccggtcagct
     8521 gcatcgtgcg gacggcctcc gcgcgcatca gctgcagcat ccgatcgacc ccgcgctcgc
     8581 cgccggccat ca
//
LOCUS       DATMSX010000284         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_89873,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000284 DATMSX010000000
VERSION     DATMSX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89873"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VN041_08660"
     CDS             complement(<1..889)
                     /locus_tag="VN041_08660"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWU29150.1"
                     /translation="MTDKSMDLGGVVVATTLAFKEDASAPAGLAVDYDKFGEHVDFLM
                     SNGCRGVGPNGSLGEYSSLTDDERRKVIQVAVEAVDGRGIVIAGVHGVGSHQARQWAE
                     YAREDGADGVLLLPPTIYRANESEVIAHYQEVAKVGLPIMAYNNPFDTKVDLVPSLVA
                     KLAEIPEVVAIKEFSGDVRRVYEIKELCDIDIIAGADDVLFELMVNGAVGWFAGYPNA
                     FPREAVELYNLCADGNWHEAKALYEQLVAVFRWDSKTEFVQAIKLSMDICGNSYGGAT
                     RPPRGPLSDEQRAQVTADTE"
     gene            complement(961..2280)
                     /locus_tag="VN041_08665"
     CDS             complement(961..2280)
                     /locus_tag="VN041_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29151.1"
                     /translation="MSTTTEIEDQIASPGAAEARRVRKVMTSSAIGHFVEWFDFAVYA
                     YAAPVIAALFFPKTDAAAALMAVFAIYAVGFVARPVGAFLLGNLGDRFGRKRVLAGVI
                     LVMGVSTTLIGLLPTYAVIGVAAPVLLVVLRMIQGLSAAGETIGSNSFVAEHSPTARR
                     GFNVGMVYTWSNLPPVIAALLVLGLTTMLSPDAYESWGWRVPFLLGAPLAVVGLYIRT
                     RVDESPAFLEMRESRQVEKAPIRTVFREQWRSMLVCFSIAAVASLGYYSLTGYFYSYM
                     TVTIGLESSAALISNSIALLITFFTVPVAALVSDRIGRRRMLLIAGLFGAVIAVPAYL
                     LVGLGTLGAAILSQGLLGLALGLFFGPAGPAFVELFPARVRYTGASISYNLAFTIFGG
                     TAPLLATGLISLTGSTVAPAWYVVIVTVLALLVVVTMPETNRRSMRD"
BASE COUNT          412 a          800 c          944 g          378 t
ORIGIN      
        1 gctcggtgtc ggccgtgacc tgggcgcgct gctcgtcgct cagcgggccg cgcggcgggc
       61 gggtcgctcc gccgtaggag ttaccgcaga tgtccatcga cagcttgatg gcctggacga
      121 actccgtctt cgagtcccac cggaacacgg cgacgagctg ctcgtacagc gccttcgcct
      181 cgtgccagtt gccgtcggcg cagaggttgt acagctcgac ggcctcgcgc gggaaggcgt
      241 tcgggtagcc ggcgaaccag cccacggcgc cgttgaccat cagctcgaag agcacgtcgt
      301 cggcaccggc gatgatgtcg atgtcgcaga gctccttgat ctcgtagacg cggcgcacat
      361 cgccggagaa ctccttgatc gcgacgacct cggggatctc ggcgagcttg gcgaccagcg
      421 aggggaccag gtcgaccttg gtgtcgaacg ggttgttgta ggccatgatc ggcaggccga
      481 ccttcgcgac ctcctggtag tgcgcgatga cctcggactc gttggcgcgg tagatggtcg
      541 gcggcagcag cagcacgccg tcggcgccgt cctcacgggc gtactcggcc cactggcggg
      601 cctggtggct gcccacgccg tgcacgccgg cgatcacgat tccacggccg tcgacggcct
      661 cgacggcgac ctggatcacc ttgcggcgct cgtcgtcggt cagcgaggag tactcgccga
      721 gcgagccgtt cgggccgacg ccgcggcatc cgttcgacat caggaagtcg acgtgctcgc
      781 cgaacttgtc gtagtcgacg gccaggcccg ccggcgcgga tgcgtcttcc ttgaacgcga
      841 gggtcgtggc gacgacgacg ccgccgaggt ccatgctctt gtcagtcata gctgttctct
      901 ttctcatctg tcgcgccgcg gatgcggcgg ttctgtcggg ggtgtcaggg gtcggtgcgc
      961 tcagtcgcgc atcgaccgtc ggttggtctc gggcatcgtc acgacgacga gcagcgccag
     1021 cacggtcacg atcacgacgt accaggcggg tgcgaccgtc gagccggtca ggctgatcag
     1081 gccggtggcg agcagcggcg cggtgcctcc gaagatcgtg aacgcgaggt tgtagctgat
     1141 ggatgcgccg gtgtagcgca cccgggcggg gaacagctcc acgaacgcgg ggccggccgg
     1201 gccgaagaac agtccgagcg cgagtccgag cagcccctgg ctgaggatgg ccgcgccgag
     1261 cgtgccgaga ccgaccagca ggtacgccgg caccgcgatg acggcgccga agagaccggc
     1321 gatcagcagc atccgccgac ggccgatcct gtcggagacg agcgccgcca cgggaaccgt
     1381 gaagaacgtg atgagcagcg cgatgctgtt cgagatcagc gcggcggagg actccagccc
     1441 gatcgtcacc gtcatgtatg agtagaagta gccggtcagc gagtagtagc cgaggctggc
     1501 cacggcggcg atgctgaagc agaccagcat gctgcgccac tgctcgcgga acaccgtgcg
     1561 gatcggggcc ttctcgacct gacggctctc gcgcatctcc aggaaggcgg gcgactcgtc
     1621 gacgcgggtg cggatgtaga ggccgacgac ggcgagcggg gcgccgagca ggaacggcac
     1681 gcgccagccc cacgactcgt acgcgtcggg cgagagcatg gtggtcaggc cgagcacgag
     1741 cagggccgcg atcacgggag gcaggttcga ccaggtgtag accatgccga cgttgaatcc
     1801 gcggcgggcc gtgggcgagt gctcggccac gaaggagttg gagccgatcg tctcgccggc
     1861 ggcggacagg ccctggatca tgcgcagcac gaccaggagc acgggggccg cgacgccgat
     1921 gaccgcatag gtcgggagca ggccgatcag ggtcgtcgag acgcccatga ccaggatcac
     1981 gccggcgagc acgcgcttgc ggccgaagcg gtcgccgagg ttgccgagca ggaaggcgcc
     2041 gacggggcgc gctacgaagc cgaccgcgta gatggcgaag accgccatga gggcggcggc
     2101 cgcgtcggtc ttggggaaga acagcgcggc gatgacggga gcagcgtagg cgtacaccgc
     2161 gaagtcgaac cattcgacga agtgtccgat ggccgaggac gtcatgacct tgcggacacg
     2221 gcgggcctcg gctgcgccgg gcgaggcgat ctggtcttcg atctcagtgg tggtgctcat
     2281 gcggctcctt tgtcgcgtgg gggagtgata tgtaacatat cacacgctac tgcgatgcgc
     2341 cagggtggcg ggtggtgtgc gctgtgcggc gtcagctccc gtcgatgggg tgtggcgcgg
     2401 cgggcgatcg gtgtgcggcg agttctccga tgcgcaccga cgagagcacc ggtcgccggt
     2461 cgatgcccgt cggggcgccg atgatgtcct ccacgctgcg accgcacgtg cgcccctggc
     2521 acgggccgag cccc
//
LOCUS       DATMSX010000285         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_4758,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000285 DATMSX010000000
VERSION     DATMSX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4758"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VN041_08670"
     CDS             complement(<1..1348)
                     /locus_tag="VN041_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29152.1"
                     /translation="MNAAVRIAGRVGRALRETLLTLAAVGGVACVVLTILAFTGGYSL
                     IMFKTGSMSPTIPAGSVALVQRVPASDLAVGDVTTVDRAGELPITHRIIGISPGPTPT
                     QRMITMKGDANQSADPAPYTISDARIVRGSLPGLAYVIVWFGSPWVLGTITVGAALLV
                     TWAFWPRVRRPAPADAEDDHLDTTGDDAPPSPEFASRRERRLGQHVASCVAVGTLVVL
                     PSLTSAPPAGAAELISITSNGGGTHVLDAVRPYDWDLDIDAGSAPADGELTVTLRGEG
                     HGVQLSTIVRSCTTRWEGSGCPGTATTLDAAAPLTLNSVEKPLLRQATPSAVHLRISV
                     TGGGDPSGGATLVLRATALPRKRRRSTSCLHSSSSPPPRGGRRNMVGLLNGINNLLDS
                     LQQADIRQTLPPLLASSSGLSRGSNHVSLIDTLQMLGTSPSMTEERFSLSLTNLMAG"
     gene            complement(1353..2018)
                     /locus_tag="VN041_08675"
     CDS             complement(1353..2018)
                     /locus_tag="VN041_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HWU29153.1"
                     /translation="MEVGSGIPVAALRSTRRENRARARHLRSRRIRAVLAGGLVLGVG
                     FTMTLAAWTDQELASSTVQAGTFSVSSRTVQTDPFVSHGAGNPATLQLNATGLYPGVS
                     RAAWIQVQTSGSLGGTVSLTNVSASAPGAADQALLNALTAVVVQVAQPSACTPTTTSA
                     TMQPSPLTTVPTGAQLPPAALQGGGANTVTYCVVLTLPSTATTAAQGGTVAPTWTFTG
                     TTG"
     gene            complement(1991..2545)
                     /locus_tag="VN041_08680"
     CDS             complement(1991..2545)
                     /locus_tag="VN041_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HWU29154.1"
                     /translation="MDTSSAAKGLRRRRIYAVLAGGLVLGVGTAVTLAAWNDSEFAKS
                     TFTAGSFVFQGSTDGTAFADHASEAGAADLTFLVNPTNLSPGDTVYAPYAVKLTGSSA
                     ADLTQTVAIASGGTLVAADLAFDAVATTAFGCDATAFGAGTAIPTSITAGQTVNLCLK
                     VSATEDLVQGHTGTVTWKWEAVSQ"
     gene            complement(2704..2777)
                     /locus_tag="VN041_08685"
     tRNA            complement(2704..2777)
                     /locus_tag="VN041_08685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2743..2745),aa:Gly,seq:tcc)
     gene            2884..2960
                     /locus_tag="VN041_08690"
     tRNA            2884..2960
                     /locus_tag="VN041_08690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2918..2920,aa:Pro,seq:tgg)
     gene            3021..4481
                     /gene="tig"
                     /locus_tag="VN041_08695"
     CDS             3021..4481
                     /gene="tig"
                     /locus_tag="VN041_08695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWU29155.1"
                     /translation="MANSTVEKLSPTRVKLTITVTPEDLKPSIEHAYEHIAQDVQIPG
                     FRKGKVPAPIIDQRIGRGAVIEHAVNEGLDKFFREAASEHNVRIVGRPSADITQWPNE
                     KDFSGDLLVDVEVDVRPEIEIPAYDDITLTVDAVETDDAAIDAELDGLRARFGTLIPV
                     DRPAAKGDFVELDLVATIDENEIDRADGVSYEVGSGELLEGIDEAIDSLTAGEQTTFR
                     SKLVGGDHAGEEAEVSVTVKAVKERELPEADDDFAQMASEFDTIAELRDSLAERVGQQ
                     GVFTQGAAARDQLIDALLEKVEIPVPAQLIEDEVHNHLENENRLEDDVHRAEVAEASE
                     KQFRTQVLLDTIAEGANVQVSQEELSQYIVQSAAQYGMAPQEFVEALQGAGQLPALVG
                     EIARNKALASVLGKVKVVDSNGKPVDLSEFIGDVDEAEAEEAPAEEKPAKKPAAKKAP
                     AKKAADKADDAEKKPAAKKAPAKKAAAKKDEAEKAE"
     gene            4565..>4749
                     /locus_tag="VN041_08700"
     CDS             4565..>4749
                     /locus_tag="VN041_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29156.1"
                     /translation="MTAVSDDWQSRVDAVWQDADLTDDARIDAIDDLAQELGPDHPVA
                     LFERAGARDSAGRELEA"
BASE COUNT          823 a         1589 c         1669 g          668 t
ORIGIN      
        1 ctccggccat gagattggtg agagacaggc taaacctctc ctccgtcatg ctcgggcttg
       61 tcccgagcat ctgcaacgtg tcgatcaatg agacgtggtt agatcctcgg gacaagcccg
      121 aggatgacgc caagaggggg ggtaatgttt gacggatgtc tgcctgctgt agcgaatcca
      181 acaaattgtt tatcccgtta agcagcccga ccatattccg cctgccgccg cgtggcggtg
      241 gcgagctgga actgtgcaag caactcgttg agcgccgccg cttccgaggc aaggccgtgg
      301 cgcgcaggac gagcgtcgcg ccgcccgagg ggtcaccgcc accggtgacg gagatgcgca
      361 ggtgcacggc actcggggtc gcctgccgga ggagcggctt ctcgacgctg ttgagcgtca
      421 gtggcgcggc ggcgtcgagg gtggtcgccg tgccggggca tcccgacccc tcccaccggg
      481 tcgtgcacga ccgcacgatc gtggacagct gcacgccgtg gccctcaccg cgcagggtga
      541 cggtcagctc accgtccgca ggtgcggacc cggcgtcgat gtcgaggtcc cagtcgtagg
      601 gtcgcaccgc gtcgagcacg tgggtgccac cgccgttcga ggtgatcgag atcagctccg
      661 cggccccggc gggcggggcc gacgtgaggg acggcagcac gacgagcgta ccgaccgcga
      721 cgcaggaggc gacgtgctga cccaggcgac gctcgcggcg cgacgcgaac tcgggggagg
      781 gcggagcgtc gtccccggtg gtgtccaggt ggtcgtcctc ggcatctgcg ggcgccggtc
      841 tccgcacgcg cggccagaag gcccaggtga ccagcaacgc ggcaccgacg gtgatggttc
      901 cgagtaccca ggggctgccg aaccagacga tgacgtaggc gagtcccggc agcgagccgc
      961 gcacgatcct ggcgtccgag atcgtgtacg gcgcggggtc ggcgctctgg ttcgcgtcac
     1021 ctttcatcgt gatcatgcgc tgcgtcgggg tgggaccggg ggagatgccg atgatccgat
     1081 gcgtgatcgg cagctcgccc gcacggtcga cggtcgtcac gtcgccgacg gcgagatcgg
     1141 atgccgggac cctctgcacg agcgcgaccg agccggcagg gatggtcggt gacatcgatc
     1201 cggtcttgaa catgatcagc gaatacccgc cggtgaacgc gaggatcgtc agcacgacgc
     1261 aggccacccc gccgacggcg gcgagcgtga gcagcgtctc gcggagcgcg cggcccacgc
     1321 gaccggcgat gcggacggcg gcattcatcg ggtcacccgg tcgtcccggt gaaggtccac
     1381 gtcggcgcga ctgtcccgcc ctgggctgcc gtggtggcgg tcgagggcag ggtgagcacg
     1441 acgcagtagg tcacagtgtt cgcgccaccg ccctgcagag ccgcgggcgg cagctgagcg
     1501 ccggtcggca ccgtcgtcag cggcgagggc tgcatcgtcg cgctggtggt cgtcggcgtg
     1561 caagccgacg gctgcgccac ctgcacgacg acggctgtga gcgcgttcaa cagcgcctga
     1621 tcggcagcac ccggcgcgct cgcgctcacg ttcgtgaggc tgaccgtgcc gcccagcgac
     1681 ccggacgtct ggacctggat ccacgccgcc ctgctgacac ccgggtacag gccggtcgcg
     1741 ttgagctgca gcgtggccgg gttccccgcg ccgtgcgaca cgaacgggtc ggtctggacc
     1801 gtcctggacg agaccgagaa cgtccccgcc tggacggtcg acgaggcgag ctcctggtcc
     1861 gtccaggcgg caagagtcat cgtgaagccg acgcccagca ccagcccgcc ggcgagaacg
     1921 gcgcggatgc gccgcgatcg cagatgtcgc gcccgagcac ggttctcgcg gcgggtggag
     1981 cggagggcgg ctactgggat accgcttccc acttccatgt caccgttcct gtgtgaccct
     2041 gcacgaggtc ctcggtcgcc gagaccttca ggcagaggtt caccgtctgg ccggcggtga
     2101 tcgaggtggg gatcgccgtg cctgcgccga acgccgtcgc gtcgcagccg aaggcggtgg
     2161 tggccacggc gtcgaaggcc aggtcggcag cgacgagcgt tccgcctgac gcgatcgcga
     2221 cggtctgcgt gagatcagcg gccgagctcc ccgtgagctt cacggcatat ggggcgtaca
     2281 ccgtgtcacc gggggacagg ttggtcggat tcacgaggaa ggtcaggtcg gcggcgcccg
     2341 cctcggacgc atggtcggcg aacgcggtgc cgtccgtgga cccctggaac acgaacgagc
     2401 ccgcagtgaa ggtgctcttc gcgaactcgg agtcgttcca ggcggccaga gtgaccgccg
     2461 tgcccacgcc gagcacgagg ccaccggcga gcacggcgta gatcctgcgc cggcgcaggc
     2521 ccttcgcggc agatgaagtg tccatgagtt tcccttctca tcaggacagc gtgtggggga
     2581 gcacgctttc gcgtcagcgt agtgacatat cggaatatcg ggaagggaat ccgcaaccga
     2641 tgcagaacgg tgatcaaagc cggggacgag agaaggccct cgccgatgcg gcgagggcct
     2701 tgctggaggg gatgacggga atcgaacccg cgtcattagt ttggaagact aaggctttac
     2761 cattaagcta catccccgct cggcggcccg ctgagcggtg cgcctcgtaa agcatagcgg
     2821 acgtcggacg tgctctcgtc cgttagactc gtcaaggccg ttcccgtgcg cgcgggttca
     2881 agccggggcg tagctcagct tggtagagcg cccgctttgg gagcgggagg tcgcaggttc
     2941 aaatcctgtc gccccgacca cggctacaga cttcatgccc gcgccatccc gcgcggaaac
     3001 cacaaggaga acacaccagt atggccaaca gcaccgtcga gaagctgtcc ccgacccggg
     3061 tcaagctcac catcacggtc acccctgagg acctcaagcc gagcatcgag cacgcctacg
     3121 agcacatcgc ccaggacgtg cagatccccg gcttccgcaa gggcaaggtc ccggctccga
     3181 tcatcgacca gcgcatcggc cgcggtgcgg tcatcgagca cgccgtcaac gagggcctcg
     3241 acaagttctt ccgcgaggcc gccagcgagc acaacgtgcg catcgtcggc cgcccgtccg
     3301 ccgacatcac ccagtggccg aacgagaagg acttctccgg cgacctgctg gtcgacgtcg
     3361 aggtcgacgt gcgtcccgag atcgagatcc cggcctacga cgacatcacc ctcacggtcg
     3421 acgccgtcga gaccgacgac gccgccatcg acgccgagct cgacggcctg cgcgcccgtt
     3481 tcggcacgct gatccccgtc gaccgtccgg ccgccaaggg cgacttcgtc gagctggacc
     3541 tggtcgccac catcgacgag aacgagatcg accgcgccga cggcgtgtcc tacgaggtcg
     3601 gctccggcga gctgctcgag ggcatcgacg aggccatcga ctcgctgacc gccggtgagc
     3661 agaccacctt ccgctccaag ctcgtcggcg gcgaccacgc cggcgaggag gccgaggtct
     3721 ccgtgaccgt caaggcggtc aaggagcgcg agcttcccga ggccgacgac gacttcgctc
     3781 agatggcatc cgagttcgac acgatcgccg agctgcgcga cagcctcgcc gagcgcgtcg
     3841 gccagcaggg tgtcttcacc cagggcgccg ccgctcgcga ccagctcatc gacgctctgc
     3901 tcgagaaggt cgagatcccg gtccccgcgc agctcatcga ggacgaggtg cacaaccacc
     3961 tcgagaacga gaaccgtctc gaggacgacg tgcaccgcgc cgaggtcgcc gaggccagcg
     4021 agaagcagtt ccgcacgcag gtgctgctgg acacgatcgc cgagggtgcg aacgtgcagg
     4081 tctcgcagga ggagctgagc cagtacatcg tccagtccgc tgcgcagtac ggcatggctc
     4141 cccaggagtt cgtcgaggct cttcagggcg ccggccagct tcccgccctc gtcggcgaga
     4201 tcgcgcgcaa caaggcgctc gcctcggtgc tcggcaaggt caaggtcgtc gacagcaacg
     4261 gcaagccggt cgacctgtcc gagttcatcg gtgacgtcga cgaggccgag gccgaggagg
     4321 cccccgccga ggagaagccc gccaagaagc cggctgccaa gaaggctccc gcgaagaagg
     4381 ccgccgacaa ggccgacgac gccgagaaga agccggctgc caagaaggct ccggccaaga
     4441 aggccgccgc gaagaaggac gaggccgaga aggccgagtg atcgcggacc ggctctgagg
     4501 gccgatcaga tctgagagag ggcgggtgct gcggcatccg ccctctccgt atccgggagg
     4561 aatcatgact gcagtgagcg acgactggca gagccgtgtg gatgccgtat ggcaggacgc
     4621 cgacctcacc gacgacgcgc gcatcgacgc gatcgacgac ctggctcagg agctcggccc
     4681 cgatcacccg gtcgcgctgt tcgagcgcgc cggcgcccgg gacagcgccg gccgggagct
     4741 ggaggccga
//
LOCUS       DATMSX010000286         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_165362,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000286 DATMSX010000000
VERSION     DATMSX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165362"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VN041_08705"
     CDS             <1..435
                     /locus_tag="VN041_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HWU29157.1"
                     /translation="VGATVSLRAGAGVDHGPSGPAAITGEVFSITDGDPAAGTQAVIA
                     VGGLHAIITERRKPFHHLDDFRMLGLDPESADIVVVKIGYLEPELYDLAADWTLALTP
                     GGVDQDLLRLGHRHLAPGVFPFDTTGNPPLTAHVSRRQETSA"
     gene            432..>1644
                     /locus_tag="VN041_08710"
     CDS             432..>1644
                     /locus_tag="VN041_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HWU29158.1"
                     /translation="MMNFETRTGPDFGAGAPSYPGNGVPEWYRDAKLGFFVHWGLYSV
                     PAWAVQHGAGVNIPTEDAYAWHQYAEWYGNTVRIAGSPTWRRHQELYGPGTSYEDLAD
                     RWDAAAFDADAFVGELVDAGARYIVPTTKHHEGFCLWDTATTGFNAAARGPRRDLIAE
                     FHDAARRAGARFGVYFSGALDWHVSDFPPIESDTDLFRFRRHDEAFSRYAAAQLEELI
                     ERFSPDVLWNDIEWPDGGKGRDEYAVAALLERYFGAVPEGVVNDRWGVPYHGFLTREY
                     THIPDILEQPWESTRGLGYSFGFNQAEDERHSLSGVELIRMLVDIVSKNGNLLINVGP
                     AADGSIPELQRAAMRELGSWLRENGDAVYGTRPWIRFGEETGAPVRYTRSAAGVHVHA
                     LDPVAGEIALPA"
BASE COUNT          242 a          581 c          577 g          244 t
ORIGIN      
        1 gtcggcgcga cggtctcgct gcgcgcggga gccggcgtcg atcacggtcc cagcggtccg
       61 gcggcgatca ccggagaggt cttctcgatc acggacggcg atccggctgc gggcacgcag
      121 gcggtcatcg ccgtcggcgg gctccacgcg atcatcaccg agcgacggaa gccgttccac
      181 catctggatg acttccggat gctcggactc gaccccgaga gcgcggacat cgtcgtggtc
      241 aagatcggct acctcgagcc ggagctgtac gatctcgcgg ccgactggac gctcgccctc
      301 acccccggcg gggtcgacca ggacctgctg cggctggggc atcgccacct cgctccgggt
      361 gtgttcccgt tcgacaccac cggcaatccg cctctcaccg cgcacgtgag ccgacgtcag
      421 gagacctccg catgatgaac ttcgagacgc gcaccgggcc tgacttcggt gccggcgcac
      481 cgagctaccc gggcaacggc gtgcccgagt ggtatcgcga cgccaagctg ggcttcttcg
      541 tgcactgggg gctctactcg gtgccggcct gggccgtgca gcacggcgcg ggcgtgaaca
      601 tccccaccga ggacgcatac gcctggcatc agtacgcgga gtggtacggg aacacggtgc
      661 gcatcgcggg cagcccgacg tggagacggc atcaggagct gtacgggccc ggcacctcct
      721 acgaggatct cgccgaccgg tgggacgcgg cggcgttcga tgcggacgct ttcgtcggag
      781 agctcgtcga tgccggggcc aggtacatcg tgcccaccac caagcaccat gagggcttct
      841 gcctgtggga cacggcgacg accggcttca acgccgcggc gcgcggaccg cgccgcgacc
      901 tcatcgccga gttccacgac gccgcccgcc gcgccggtgc ccggttcggc gtgtacttct
      961 cgggtgcgct ggactggcac gtgagcgact tcccgcccat cgagtccgac accgacctgt
     1021 tccggttccg ccggcacgac gaggcgttct cgcggtacgc ggcggcccag ctggaggagc
     1081 tgatcgagcg gttctcgccc gatgtgctct ggaacgacat cgagtggccg gacggcggca
     1141 aggggcgcga cgagtacgcc gtcgccgcgc tgctggagcg ctacttcggc gcggtgccgg
     1201 agggcgtcgt caacgaccgg tggggcgtcc cgtaccacgg gttcctcacg cgcgagtaca
     1261 cgcacatccc cgacatcctc gagcagccct gggagtccac gcgcggcctg ggatactcgt
     1321 tcggcttcaa ccaggcggag gacgagcgcc attcgctctc cggcgtcgag ctgatccgga
     1381 tgctggtcga catcgtctcc aagaacggga acctgctgat caacgtcggc cccgccgccg
     1441 acggctcgat ccccgagctg cagcgggcgg cgatgcgtga gctgggcagc tggctgcgcg
     1501 agaacggcga cgccgtgtac ggcacgcggc cgtggatccg cttcggcgag gagacgggag
     1561 ctcccgtgcg ctacacgcgc tcggctgccg gcgtgcacgt ccacgccctc gaccccgtgg
     1621 ccggcgagat cgcgctgcct gcgg
//
LOCUS       DATMSX010000287         7755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_89927,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000287 DATMSX010000000
VERSION     DATMSX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89927"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VN041_08715"
     CDS             complement(<1..1276)
                     /locus_tag="VN041_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29159.1"
                     /translation="MTTSSAAGKRASQRRIAFATIIGTTIEWYDFFIYATSAGLVFAQ
                     LFFEPAGKEIALPLSFATVGISFLFRPLGAFLAGHFGDVIGRRAMLVVTLVLMGVATT
                     LIGTLPTYSQIGITAPILLLLLRILQGLSAGGEWGGAVLMAVEHAPADRRGRAGSWPQ
                     LGVPLGFLLASGMTALMTGVISPGEAYLEWGWRVPFLVSIVLIVVGFVIRRSVEESPV
                     FAEIAERGKQAGLPIVTLFRRHWHLVLLAALVFVGNGTLGYMTTGGFLNSYAISVGHL
                     ETTSVLLAAVFAAVVWFFSTLLAGYLADAIGRRSTFLIGFGLQAVIVFPVFWLVDAGG
                     LPGLYVALLLISTGLGLTYGPLAAWYAEIFPASIRFSGVSITYAIGAVLGGAFAPMIA
                     QMLLGATGTTAAVSAYLLLATLLGAGATLCLRD"
     gene            1424..2389
                     /locus_tag="VN041_08720"
     CDS             1424..2389
                     /locus_tag="VN041_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU29160.1"
                     /translation="MVDVSLRQLELFAALPHFPTLSAAAAHLHISESALSQAITSLEN
                     AVGEQLCVRRKARGLTLTPTGQDFARRARQIVAETHELVLGAGREQELQGPVRLGCYS
                     SFATNVVPELLEGFPKRHPGVTIEIMVGTNEELLSALEAGRLDVALVYDVSLPVGYRR
                     RRIYATELEAHLHPEHPLAVHDTVDLAELADEPYILYDATPGIRNVTDAFTARGLEPR
                     IAAKVTQIILVEALVGRGLGYGLLMSRPNALPMSSEGRPIAIRPFDPPVTVSHVVGIW
                     PADMKLTPRASALLDFAVEKLGGHGRADAIGVPGASEEATESAPG"
     gene            complement(2400..3182)
                     /locus_tag="VN041_08725"
     CDS             complement(2400..3182)
                     /locus_tag="VN041_08725"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29161.1"
                     /translation="MTDGTMGAWSERRTRTPLTDSERILSGVLRHVREAVASDSTLPG
                     EHRLAEALGCTRQQIRHALADLENQGIVVRRQGAATTIDPLTLRLSARFEARVEYGDV
                     LARMGYTPSVEVLSASFEPIPDEIAALLHPQIEPVGAHIRLRWYADDIPAMVGEYILP
                     LPAGMERPFIDPTRPVFDIAQDLWSEAIAWEVVSAGVTALTAEEAEHLEQPEGEVAKM
                     WTIVGASLSGKRISYSREVHHRDLVTYSFIRSVKEPWKIGPR"
     gene            3342..4547
                     /locus_tag="VN041_08730"
     CDS             3342..4547
                     /locus_tag="VN041_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU29162.1"
                     /translation="MTTTDSIRGIPLWDGHAYRGASSLEWSGDRIEAVETHDERLDGY
                     CVIPGLIDTHVHLGAYSGSGRADWSSWPLITPWEEQVFHIANNAQKAARAGVTTLRDL
                     SGDERQLAVKRAFDEGIQQGPRILVHGAVGMTAGHGDLFIPPHYPHRGRTADSPDECR
                     KLVREYARMGADGIKIFTTGGVLSMGDKVGWRNQTLDEIRATIDEAHALSMPVAAHSH
                     SAEGNEIALSVGVDSIEHGTGLEPDQWDRLIERNISVAPTLLINDAIADGRVAVGEEA
                     QAKAKDVVAYRDSLFGDAGRAGIRFVLGTDANGVFVQFGDQMEEVRLMARMFGWTAER
                     ALRAATSDAADVVRMSDRIGSLAAGHGADFVVLRGRPWEDIEDLRLENIIAVVSRGRL
                     VHGELPTAG"
     gene            4601..6208
                     /locus_tag="VN041_08735"
     CDS             4601..6208
                     /locus_tag="VN041_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU29163.1"
                     /translation="MNISTRHASARWAAALGAAALGAGLLAGCSAGNGGGGGGGGTTK
                     DELYIAVQDDPVCLDPQQVTLTTALNIGRQLVDSLVDQNPETGEIVPWLAKSFEANDN
                     LTEFTFTLRDDVTFSDDSKLTPETVKANFDALAALGSTAALASQYLAGYQGTTVVDGS
                     TVKVAFAEPNAQFLQGASTITLGLLSDKSAAMTAEERCQHPVGSGAFVLDSYTSNDSV
                     VVTARKGYDWGSALRTRKGDAGVKKITFAVTPEPGVRTGGLQTGEFDMILDLPAADEK
                     RFGTDQYEIYARANPGVPHSLVPNTDRPIVSDPAVREAMILATNRAEIKDLTGSSKVP
                     AVTGVLSSATPSFLAQKDALAYDLKGAQKLLDDAGWKAGAGGIREKDGTKLSVSVTAF
                     YGQDVLEATQAQLAKAGIDLKINMVTAGDFFGAVASKDFDFLGAGLTRTDPDVLRVMF
                     SSASKARWAVVTDPELDEQLATQAETADPKARTAILEDVQKRIVEKAYLVPLLEVAQV
                     HASQKGVSGVEFDSSSRIILHDVTVKG"
     gene            6253..7266
                     /locus_tag="VN041_08740"
     CDS             6253..7266
                     /locus_tag="VN041_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29164.1"
                     /translation="MKATLRVLRYIAPKLAQFVFVVLATYTVVFLLITFLPGDPLLAA
                     LASKSGGDTIVDPEVLERLRQQYGLAGTPWEQYWHRLGMLVHGDLGISIATGQPVATM
                     IARTLPNSVEIALIALVIAIVIAVLFTFLAFLAPVRWLRNLLLQIPPFGVAIPAFLTG
                     LLLISFFSFTLGWLPSSGSRQPGSAILPAVTLALPVGAILFQVFSAAVLEAQASQHVF
                     TAVAKGVPPWRIFTAHLLRNALLPTITVLGLTIGYLAAGTAVVETVFSRDGIGRMTVN
                     AVLARDINVIQGIVLMIAAVYATVNLVVDLAYGVIDPRTRRSGRRPARTAQPADEPKE
                     VTA"
     gene            7263..>7755
                     /locus_tag="VN041_08745"
     CDS             7263..>7755
                     /locus_tag="VN041_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU29165.1"
                     /translation="MRLLRQPGFVISLLVLLLVAVAIVAPSLLSTHDPYLSVPKDRLL
                     PPSAEYLFGTDNLGRDLYSRVVHGTALSIVSAALAVAVGVIVGGLVGLASGFLGGPVD
                     FAIMRLVDVLVAIPGILLALIVVATLGFGPVSIGLGVGLGAAGSFARVMRSQVLRVRN
                     EEYV"
BASE COUNT         1219 a         2707 c         2672 g         1157 t
ORIGIN      
        1 gatcgcgcag gcacagcgtg gcgccggccc ccagcagtgt ggcgagcagc agataggcgg
       61 agaccgccgc ggtggtgccg gtcgcgccca gcagcatctg ggcgatcatc ggcgcgaacg
      121 ccccgcccag caccgcgccg atggcgtacg tgatcgagac gcccgagaag cggatggatg
      181 ccgggaagat ctcggcgtac cacgccgcca gcggtccgta ggtgaggccg agacccgtcg
      241 agatcagcag cagtgccacg tagaggccgg ggagtccgcc tgcgtcgacg agccagaaga
      301 ccgggaagac gatgacggcc tgcaggccga agccgatgag gaaggtgctg cgccggccga
      361 tcgcgtccgc gaggtatccg gccagcagcg tggagaagaa ccacacgacg gcggcgaaga
      421 cggcggcgag cagcaccgag gtggtctcga gatggccgac gctgatcgcg tagctgttca
      481 ggaagccgcc ggttgtcatg tagccgaggg tgccgttgcc gacgaagacg agggccgcga
      541 ggagcaccag gtgccagtgg cgccggaaga gcgtgacgat cggcaggccg gcctgctttc
      601 cgcgctcggc gatctcggcg aacaccgggc tctcctccac ggaacggcgg atcacgaagc
      661 ccaccacgat cagcacgatg ctcaccagga acggcacgcg ccaaccccac tccaggtatg
      721 cctcgccggg ggagatcacg cccgtcatca gtgcggtcat gcccgacgcc agcaggaatc
      781 cgaggggcac cccgagctgc ggccaggagc cggcgcggcc gcggcggtcg gcgggcgcgt
      841 gctccacggc catcagcacc gcgccgcccc actcgccgcc ggcggacagg ccctgcagga
      901 tgcggagcag gagcagcagg atgggcgccg tgatgccgat ctgcgagtag gtggggaggg
      961 tgccgatgag agtcgtcgcc acgcccatca ggaccagggt gacgaccagc atcgcccgcc
     1021 gtccgatgac gtcgccgaag tggccggcga ggaaggcgcc cagcgggcgg aagaggaacg
     1081 agatgccgac ggtagcgaat gacagcggga gcgcgatctc cttgcctgcg ggctcgaaga
     1141 agagctgggc gaacaccagg ccggcgctgg tggcgtagat gaagaagtcg taccactcga
     1201 tggtcgtgcc gatgatggtc gcgaaggcga ttcggcgctg cgaggcgcgc tttcctgcgg
     1261 cggaggaggt ggtcatcgtt gtccctaacg tcattgtcat cgccctccgg gtgagggctg
     1321 ggatgcgggt ttctcccatc gtgcggtgcg cacgactgcg tgtaaatcac agttattcgc
     1381 ggatatagtt cgattcgact gaagaatcgg agaaggagtc gccgtggtcg atgtgagcct
     1441 gaggcagctg gagctgttcg ccgcgctgcc ccatttcccc acgctcagcg cggccgccgc
     1501 acacctgcac atctcggagt cggcgctgtc gcaggccatc acgagcctgg agaacgcggt
     1561 gggcgagcag ctgtgcgtgc ggcgcaaggc gagggggctc acactcacgc cgaccggcca
     1621 ggacttcgcc cggcgggcga ggcagatcgt cgcggagacc catgagctcg tcctcggcgc
     1681 ggggcgggag caggagctgc aggggccggt gcggctcgga tgctattcga gcttcgccac
     1741 gaacgtcgtg cccgagctgc tcgagggatt cccgaagcgg catccggggg tgacgatcga
     1801 gatcatggtc ggcaccaacg aggagctgct ctccgcgctg gaggcggggc ggctggacgt
     1861 ggcgctcgtg tacgacgtgt cgctccctgt cggataccgg cgtcgccgga tctacgcgac
     1921 cgagctcgag gcgcacctgc atcccgagca tccgctcgcc gtgcacgaca cggtcgacct
     1981 cgcggagctg gccgacgagc cctacatcct gtacgacgcg acgcccggca tccgcaacgt
     2041 gaccgacgcg ttcaccgcgc gcggtctcga gccgcggatc gcggcgaagg tcacccagat
     2101 catcctcgtc gaggctctcg tggggcgtgg tctcggctac ggcctgctca tgtcccggcc
     2161 gaacgcgctc ccgatgagct cggaggggcg gcccatcgcg atccgcccgt tcgacccgcc
     2221 ggtgacggtg tcgcacgtgg tggggatctg gccggcggac atgaagctca cgccgcgtgc
     2281 gtcggcgctg ctcgacttcg ccgtggagaa gctcggcggt cacggccgcg cggatgccat
     2341 cggggttcca ggggcctccg aggaggcgac cgagtccgcg cccgggtgag ggctgcgcgt
     2401 cagcgcggtc cgatcttcca cggctccttc acggagcgga tgaacgagta ggtgaccagg
     2461 tcgcggtgat gcacctcgcg cgaatacgag atgcgcttgc cgctgagcga tgcgcctacg
     2521 atcgtccaca tcttcgcgac ctcgccctcg ggctgctcga ggtgctcggc ctcctcggcc
     2581 gtgagggccg tgacgccggc ggagacgacc tcccacgcga tggcctcgga ccagaggtcc
     2641 tgcgcgatgt cgaagacggg gcgggtgggg tcgatgaacg gtcgctccat gccggcgggg
     2701 agcgggagga tgtactcgcc gaccatcgcg gggatgtcgt cggcgtacca gcgcagccgg
     2761 atgtgcgcgc cgaccggctc gatctgcgga tgcagcagag cggcgatctc gtccgggatg
     2821 ggctcgaagg atgccgagag cacctcgacc gagggggtgt accccatgcg ggccaggacg
     2881 tcgccgtact ccacgcgcgc ctcgaagcgg gcgctcagtc gcagggtgag cgggtcgatg
     2941 gtcgtcgcgg ccccctggcg gcggacgacg atcccctggt tctccagatc ggccagtgca
     3001 tggcggatct gctgccgggt gcatccgagc gcctcggcga gccggtgctc gccgggcaga
     3061 gtgctgtcgg aggcgaccgc ctcgcggacg tgcctcagca cgccgctgag tatgcgttcg
     3121 ctgtcggtca gcggggtgcg cgtcctgcgc tcgctccacg cgcccatcgt gccgtcggtc
     3181 atcgccgtcc ccctgctcca ggcaagcata ttcggcccgg ggccgtcagt cgccaccatc
     3241 gtcacaacta gtcaacaagt tgcgtaatga tcgaccaaac ctatcaacaa gtggcgggag
     3301 atctgctagc gtcagtcaac ccgcacacgg aggagtcagc agtgaccacg acggacagca
     3361 tccgaggcat ccctctctgg gacggccatg cctaccgcgg ggcgagcagc ctcgagtggt
     3421 cgggcgatcg catcgaggcg gtcgaaaccc acgacgagcg gctcgacggg tactgcgtca
     3481 tccccggtct gatcgacacg cacgtgcacc tgggtgcgta ctcgggctcc ggacgcgccg
     3541 actggtcgtc atggccgctc atcaccccgt gggaggagca ggtcttccac atcgccaaca
     3601 acgcgcagaa ggccgcccgc gccggcgtga ccaccctgcg cgacctgtcc ggcgacgagc
     3661 ggcagctggc cgtgaagcgc gccttcgacg agggcatcca gcagggcccc cgcatcctgg
     3721 tgcacggcgc cgtcggcatg accgccggtc acggcgacct gttcatcccg ccgcactacc
     3781 cgcaccgcgg acgcaccgcc gacagccctg acgagtgccg caagctcgtg cgcgagtacg
     3841 cgcgcatggg cgccgacggc atcaagatct tcaccaccgg cggcgtgctc tccatgggcg
     3901 acaaggtcgg ctggcgcaac cagacgctgg acgagatccg cgcgaccatc gacgaggcgc
     3961 acgcgctgag catgcccgtc gccgcgcaca gccactccgc cgagggcaac gaaatcgccc
     4021 tgtcggtcgg ggtcgactcc atcgagcacg gcaccgggct cgagcccgac cagtgggatc
     4081 gcctcatcga gcggaacatc tcggtcgcgc cgaccctgct catcaacgac gccatcgccg
     4141 acggccgcgt cgcggtcggc gaagaggccc aggcgaaggc gaaggacgtc gtcgcctacc
     4201 gcgactccct gttcggcgac gccggccgcg caggcatccg cttcgtcctc ggcacagacg
     4261 cgaacggcgt cttcgtgcag ttcggcgacc agatggagga ggtgcgcctg atggcgcgga
     4321 tgttcggctg gaccgccgag cgtgcgctgc gcgccgccac ctccgacgcc gccgatgtgg
     4381 tccgcatgtc cgaccgcatc ggttcgctcg ccgccggtca cggagccgac ttcgtcgtgc
     4441 tgcgcggccg gccctgggag gacatcgagg acctgaggct cgagaacatc atcgccgtcg
     4501 tgtcacgagg tcgtctcgtg cacggcgagc tgcccaccgc aggctgaccg cctgcctgaa
     4561 tccgctgtac ccgaaactgc tggactctga gaggaagatc gtgaacatct cgacacgaca
     4621 tgcatccgca cgctgggccg cggccctcgg cgccgccgcc ctcggcgccg gtctccttgc
     4681 cggctgctcc gccggcaacg gcgggggcgg cgggggtggc ggcaccacca aggacgagct
     4741 gtacatcgcc gtgcaggacg acccggtgtg cctggacccg cagcaggtga ccctgaccac
     4801 cgcactgaac atcgggcggc agctggtcga ctccctggtc gatcagaacc ccgagaccgg
     4861 cgagatcgtg ccctggctcg ccaagagctt cgaggcgaac gacaacctca ccgagttcac
     4921 tttcacgctg cgcgacgacg tcaccttcag cgacgactcg aagctgacgc ccgagaccgt
     4981 gaaggcgaac ttcgatgccc tggccgccct cggcagcact gctgcgctgg cgtcgcagta
     5041 cctggccggc taccagggca ccaccgtcgt ggacggctcc acggtcaagg tcgcgttcgc
     5101 cgagccgaac gcgcagttcc tgcagggcgc cagcaccatc acgctcggac tgctctccga
     5161 caagtccgcc gcgatgaccg ccgaggagcg ctgccagcat ccggtggggt ccggcgcgtt
     5221 cgtcctggac agctacacct ccaacgactc cgtcgtggtg accgcgcgca agggctacga
     5281 ctggggctcc gcgctgcgca cccgcaaggg cgacgccggg gtcaagaaga tcaccttcgc
     5341 cgtgacgcct gagcccggtg tccgcaccgg cggtctgcag accggcgagt tcgacatgat
     5401 cctggacctg cccgcggccg acgagaagcg cttcggcacc gaccagtacg agatctacgc
     5461 gcgcgccaac cccggcgtcc cgcactccct ggtgccgaac accgaccgcc cgatcgtctc
     5521 cgacccggcc gtgcgcgagg cgatgatcct cgccacgaac cgcgccgaga tcaaggacct
     5581 caccggttcg agcaaggtcc cggcggtgac cggtgtgctc agctccgcca ccccgtcgtt
     5641 cctcgcccag aaggacgcgc tcgcgtacga cctcaagggc gcccagaagc tgctcgacga
     5701 cgcgggctgg aaggccgggg ccggcggcat ccgcgagaag gacggcacca agctgtccgt
     5761 ctcggtgacg gcgttctacg gtcaggacgt gctcgaggcg acgcaggcgc agctggcgaa
     5821 ggccgggatc gacctgaaga tcaacatggt caccgccggc gacttcttcg gcgccgtcgc
     5881 ctccaaggac ttcgacttcc tcggcgcggg cctgacgcgc accgaccccg acgtgctgcg
     5941 ggtgatgttc tcgtccgcct ccaaggcgcg ctgggccgtg gtgaccgacc ccgagctcga
     6001 cgagcagctg gccacgcagg ccgagaccgc cgacccgaag gcccgcaccg ccatcctgga
     6061 ggacgtgcag aagcgcatcg tcgagaaggc gtacctcgtg cccctgctcg aggtcgccca
     6121 ggtgcacgcc tcgcagaagg gcgtctccgg cgtcgagttc gactcctcgt cgcggatcat
     6181 cctgcacgac gtcaccgtca agggctgagc cggacacggc gggaaggtga tcgggatgac
     6241 gggagcgaaa ccgtgaaagc gacgctgcgc gtgctgcgct acatcgcacc gaagctggcg
     6301 cagttcgtct tcgtggtgct cgcgacgtac accgtcgtgt tcctcctgat caccttcctt
     6361 cccggcgatc ccctgctcgc cgctctcgcc agcaagagcg gcggcgacac gatcgtcgac
     6421 ccggaggtgc tcgagcggct ccggcagcag tacggtctgg ccggcacccc gtgggagcag
     6481 tactggcatc gcctcgggat gctcgtgcac ggcgacctgg gcatctcgat cgccaccggt
     6541 cagcccgtgg cgacgatgat cgcgcggact ctgccgaact cggtggagat cgccctcatc
     6601 gccctcgtga tcgcgatcgt gatcgccgtg ctcttcacgt tcctcgcctt cctcgccccg
     6661 gtgcgctggc tgcgcaacct gctgctgcag atcccgccct tcggagtcgc catcccggca
     6721 ttcctgaccg gtctgctgct gatcagcttc ttctccttca cgctcggctg gctgccgtca
     6781 tcgggcagtc gccagccggg cagcgccatc ctccctgcgg tcacgctggc tctgccggtc
     6841 ggggccatcc tgttccaggt gttctctgcg gccgtgctcg aggcgcaggc gagccagcac
     6901 gtgttcaccg cggtggccaa gggcgtgccg ccgtggcgga tcttcaccgc gcacctgctg
     6961 cgcaacgcgc tgctgccgac gatcaccgtg ctcggcctga ccatcggata cctcgcggcg
     7021 ggcaccgccg tggtcgagac ggtgttctcg cgcgacggca tcgggcggat gacggtgaac
     7081 gccgtgctcg cccgtgacat caacgtcatc cagggcatcg tgctgatgat cgccgcggtc
     7141 tacgcgacag tgaacctcgt ggtcgatctg gcgtacggcg tgatcgaccc ccgcacgcgg
     7201 cgctccggac gccggcccgc gcgcaccgcg caacccgcgg acgagccgaa ggaggtcacc
     7261 gcatgagact gctccgtcag cccggattcg tgatcagcct gctcgtgctg ctgctggtcg
     7321 ccgtcgcgat cgtcgcaccc tcgctgctgt ccacgcacga cccctacctg tcggtcccca
     7381 aggaccggct gctgcctccc agcgccgagt acctcttcgg aaccgacaac ctcgggcggg
     7441 acctgtactc ccgtgtggtg cacggcacgg cgctctccat cgtctcggct gcgctcgcgg
     7501 tggcggtcgg agtcatcgtc ggcggcctgg tggggctggc atccggattc ctcggcggcc
     7561 ccgtcgactt cgcgatcatg cggttggtcg acgtgctcgt cgcgatcccg ggcatcctgc
     7621 tcgccctcat cgtcgtcgcc actctcggct tcggcccggt ctcgatcggg ctcggcgtcg
     7681 gcctgggcgc cgcgggcagc ttcgcccgcg tgatgcgctc gcaggtgctg cgggtgcgca
     7741 acgaggagta cgtcg
//
LOCUS       DATMSX010000288         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_184348,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000288 DATMSX010000000
VERSION     DATMSX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184348"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..602
                     /locus_tag="VN041_08750"
     CDS             237..602
                     /locus_tag="VN041_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29166.1"
                     /translation="MKPSAAPVPVILVGGGGIIAPETLDGVSELIRPDHFGAANAVGA
                     ALGDVAGQVEKIYRVDGTEAGRRAAREDASRDAVERARLAGAASSTIEVVAVEELPAA
                     YSDGQQVLIRARAVGRLAD"
     gene            complement(656..1693)
                     /locus_tag="VN041_08755"
     CDS             complement(656..1693)
                     /locus_tag="VN041_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWU29167.1"
                     /translation="MRFPATLRSARRAPLLQVLKSAAATIAAWLIAGWVLPGPLPVFA
                     AIAALLVVQPSVNQSLSKAIERSAGVILGVIIAVLLTVVLGAQSWVVLAAVVVAMLAA
                     WAMRASTGTGNQVAISAVLVLALGASNPDYAVDRIIETLIGAAIGFVINIAIVPPVLT
                     APARRDIGMLGRELAASLDRLAEALSANRSGPQLQELMLEARLLRPMKDAAEASLEEA
                     EESLALNPRRGRHRDDLTALRALFERISPVVTQVIGMTRAYIDHYEDSLAQDPAVRAI
                     GDQLRRAGHDMRLAVQTADVAPEPDAMTSTIPALTTPLVVRPPASDHWILVGSLMEDL
                     RRIHGELRDEA"
     gene            complement(1880..2590)
                     /gene="deoD"
                     /locus_tag="VN041_08760"
     CDS             complement(1880..2590)
                     /gene="deoD"
                     /locus_tag="VN041_08760"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWU29168.1"
                     /translation="MSTHIGAASGEIAPIVLFPGDPLRAKWIAENFLDGAQLYSEVRG
                     MLGFTGTWNGHRVSVQGSGMGQPSMSIYANELFDSYDVQTIVRVGSCGGLTERVSMRD
                     IIIANGACTDSGINRTRFHGLDYAPVADFSLLRAAVEASEALSLQGRTHVGLLYSSDQ
                     FYTTRPELTEPLVAHGALAVEMETSALYTLAAYRQRRALSICTVSDHLVTHEETTSQE
                     REQTFGDMIEIALRAATA"
     gene            2643..>2950
                     /locus_tag="VN041_08765"
     CDS             2643..>2950
                     /locus_tag="VN041_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29169.1"
                     /translation="MDVERMLQQERRMLWVTAVCFAVGLLAGAVALWGGTRPFSGDGS
                     VLMPIAVVAGIIAAAAFAVSTRMHRAGETAPMPTWQAVVSDISAVAVALALVGVAGLG
                     V"
BASE COUNT          425 a          999 c         1105 g          421 t
ORIGIN      
        1 ggccgcggcc gaggggatgc acctgatcat cagcatccct cgggcccgac agcgtcggct
       61 accggctgct gcaggagggc ctggccttcg gcggcggcac gctcaccgcg accgacatcg
      121 ccctgcgggt cggagcggcg cagatcgcgg ggattgccac cccggacgtg gatgacgcgg
      181 tcgcacggac cgcctgggac gggatccgtg cgcttctcga ggacagcgtc gacaggatga
      241 agccgagcgc cgcgcccgtg ccggtgatcc tcgtgggcgg tggtgggatc atcgcgcccg
      301 agacgctgga cggagtcagc gagctcatcc ggcccgatca cttcggggcg gccaacgccg
      361 taggagctgc cctgggcgac gtcgcggggc aggtcgagaa gatctaccgg gtcgacggca
      421 ccgaggccgg ccgcagggcg gcgcgcgagg acgcgtcacg ggatgccgtg gaacgcgccc
      481 gcctggccgg cgccgcgtcc tccacgatcg aggtcgtcgc ggtcgaggaa ctgcccgcgg
      541 cgtactccga cggccagcag gtgctgatcc gtgcgcgtgc cgtggggcgg ctcgcggact
      601 gacgacgtgc ggcgcgtccg ggaggaggtc cccggcagga tctcctcccg agcgttcagg
      661 cctcgtcgcg cagctcgccg tggatgcggc gcagatcctc catgagcgag ccgaccagga
      721 tccagtgatc ggatgccggc ggacgcacca ccagcggggt ggtcagcgcc gggatcgtcg
      781 aggtcatggc atccggctcc ggggcgacgt cggccgtctg cacggcgagc cgcatgtcgt
      841 gtcctgcgcg gcgcagctga tcgccgatcg cgcgcacggc ggggtcctgg gcgagggagt
      901 cctcgtagtg gtcgatgtac gcgcgcgtca tgccgatcac ctgggtgacc acgggggaga
      961 tgcgctcgaa cagggcgcgc agcgcggtca gatcgtcgcg gtgccttccc cggcgcgggt
     1021 tcagggcgag tgactcctcg gcctcctcga gcgaggcctc cgcggcatcc ttcatcggtc
     1081 gcagcagacg tgcctcgagc atcagttcct gcagctgcgg accgctgcgg ttcgcggaga
     1141 gcgcctccgc gaggcggtcg agggaggccg cgagctcgcg ccccagcatc ccgatgtcac
     1201 gccgggcggg cgcggtgagg accggcggca cgatcgcgat gttgatcacg aagccgatgg
     1261 ccgcgccgat cagggtctcg atgatccggt cgacggcgta gtccgggttc gacgcgccca
     1321 gggccagcac gagcacggcc gagatcgcga cctggttccc cgtgccggtg gatgcccgca
     1381 tcgcccaggc cgccagcatc gcgacgacga ccgcggccag cacgacccag ctctgcgcgc
     1441 ccagcaccac cgtcagcagc accgcgatga tcacgccgag gatcacgccg gcgctgcgct
     1501 cgatcgcctt cgacagcgac tggttcacgc tgggctgcac gaccagcagg gcggcgatcg
     1561 ccgcgaacac cggcagcgga cccggcagca cccagcccgc gatcagccag gccgcgatcg
     1621 tggcggccgc cgacttcagc acctgcagca gcggggcccg tcgagccgag cggagcgtgg
     1681 cggggaagcg catgggacaa cggtagccgg gagtggtgtc agcccgggtg ccggtgtcgc
     1741 tcggtgcctc tcctcgcgcc ccgtctcggg aggagatctc agcgatggga ggaggaatcc
     1801 accagatgtc tcctccccag cgtgagatct cctcccgaac cgccgcgtcg aacgcagcga
     1861 agacgtctgc gacgaaccct caggcggtcg cggcgcgcag cgcgatctcg atcatgtcgc
     1921 cgaaggtctg ctcgcgctcc tgtgacgtcg tctcctcgtg ggtgacgagg tggtcggaca
     1981 ccgtgcagat gctcagggcg cggcgctgcc ggtaggcggc gagcgtgtac agcgcgctgg
     2041 tctccatctc gacggccagg gcaccgtgcg cgaccagcgg ctcggtcagt tcgggacggg
     2101 tcgtgtagaa ctggtcgctc gagtagagca ggcccacgtg cgtgcggccc tgcagggaga
     2161 gcgcctccga ggcctcgacc gctgcgcgca gcagcgagaa gtccgcgacc ggggcgtagt
     2221 cgagtccgtg gaagcgcgtg cggttgatgc ccgagtcggt gcaggcgccg ttggcgatga
     2281 tgatgtcgcg catgctcacg cgctcggtca gaccaccgca cgagccgacg cgcacgatgg
     2341 tctgcacgtc gtaggagtcg aacagctcgt tggcgtagat cgacatcgac ggctggccca
     2401 tgcccgagcc ctgcaccgac acgcggtgcc cgttccacgt gcccgtgaag cccagcatcc
     2461 cgcgcacctc gctgtacagc tgcgcgccgt cgaggaagtt ctcggcgatc cacttcgccc
     2521 gcagcgggtc tccggggaac aggacgatgg gggcgatctc gccggacgcg gcgccgatgt
     2581 gggtgctcat gaacccaggg tatgtccccg cggatgtccc cctgtggtgg ttgcatgaag
     2641 tcatggacgt ggagcggatg ctgcagcagg agaggcggat gctgtgggtg accgcggtct
     2701 gcttcgccgt gggcctgctc gccggcgccg tcgcgctgtg gggcgggacg cgcccgttca
     2761 gcggcgacgg ctcggtgctg atgcccatcg ccgtggtcgc cggaatcatc gcggcggcgg
     2821 cgttcgcggt gagcacccgg atgcatcgcg ccggcgagac ggcgccgatg cccacctggc
     2881 aggcggtggt ctccgacatc agcgcggtcg cggtcgcgct cgcactcgtc ggggtggcag
     2941 gactgggagt
//
LOCUS       DATMSX010000289         4381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_108736,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000289 DATMSX010000000
VERSION     DATMSX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108736"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VN041_08770"
     CDS             <1..722
                     /locus_tag="VN041_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWU29170.1"
                     /translation="GRRVWSDPDAAAFRSVRFRRASAALAASGAHPRHHRRRRRSAAS
                     EERFSMFRIRTALAGAVVAVAAVLVVAAPASAHDQIVSSTPAADEQLTTAPDQVVLTF
                     SNNLLALSDNSGTAMTVVDEQGTDWVAGAPTVAADTVTVPLKPGMPNGAYDVTWKVVS
                     SDGHPTSGEYSFSIAAAEEPTPSTGPSESAQPTAPASTQAPAESAETESAPWPLLIGL
                     GVVLLIAIVVVIVIAARKRRN"
     gene            890..1906
                     /gene="corA"
                     /locus_tag="VN041_08775"
     CDS             890..1906
                     /gene="corA"
                     /locus_tag="VN041_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWU29171.1"
                     /translation="MAIIDNGIYVDGVRTENPASLDETFELMRSRGGMGWIGLYRPDG
                     AELASVANEFGLHELAVEDALVGHQRPKIERYGEHLFVVLRPARYLDAEEEVEFGEVH
                     VFVGPDFVVTVRHAEAPDLARVRHRLESEPELLARGPEAVLYAILDEVVDQYAPVLAG
                     LENDIDEIESQLFVEDVDATQRIYELGREVIDFQRAVQPLTSMVEALLRGGVKYAADE
                     ELQRYLRDVHDHAIRVAERSASFRSILDNALTVASTIVARRQNDEMKRMTEESIRQGD
                     QMKRISSWAAILFAPTLIGAIYGMNFRVMPELHWTFGYPLAIAAMVAFGGGLYLVFKR
                     KGWL"
     gene            1903..3087
                     /locus_tag="VN041_08780"
     CDS             1903..3087
                     /locus_tag="VN041_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29172.1"
                     /translation="MSSPPRTRADQRGADLWFRLMGLPTFVPLRRWFTDLPRRVAPLI
                     ASVTIARFLLQDLVDDAIEVAVEFFGTDEWAIFASVLVLIGVALVIAWTAYALLRGLL
                     RRLRPPVGATVATAVIVLCVAGLIIGGYLMTPDATVGPVLSALALVLGCVLFTGMGGG
                     ALLTWASRLAVRNVSAIGYMASIALPVILMLVVFAFFSGEVWQMASALGWRSIWTLAL
                     VVGALAVVVVLSVCAKELDEDMHVQGHEHRLALVVGTPAEGRMQEDTLHRPLHRAQRI
                     NLLLVMAVAQVLQAVFFAALLWMLLVVIGAIAIPYGVVELWVGAGAASMPLEVVRIEV
                     ASATLPLTVNLLKAAALLSVIATLPFVFSAVSEARYRERFFDPIMADIRRAIVVRDAL
                     RA"
     gene            complement(3109..4176)
                     /locus_tag="VN041_08785"
     CDS             complement(3109..4176)
                     /locus_tag="VN041_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HWU29173.1"
                     /translation="MQLSPVQTLPSRLVRRGVDVFPGVILTAAIALVSTGIGAILPVL
                     GSAVPAIIIGVLLSIVRPRVMAGRERTDARIQPGIAYSGKFLLQLAVVVLGVQLSIGA
                     IAKVGLESLPIMLTTLIVCLVGAWLLGRMLGTERRLTTLIGVGTGICGASAIAAVSPV
                     IGAASAEIAYAVSTIFLFNILAVLIFPLIGHALNLDPHTFGLFAGTAVNDTSSVVAAA
                     GVFATTALGFAVVVKLVRTLMIIPISIGLSIMEARRSADGQKLTGRRIAKLVPWFLIG
                     FVVVALINSTGLIPDAPRQVLVHASVFLIAMALAGIGLSTDIPALRRAGWRPLALGGM
                     LWMLVTLTALATIAATAALRG"
BASE COUNT          654 a         1460 c         1596 g          671 t
ORIGIN      
        1 ggggccgccg cgtctggtct gaccccgatg ccgccgcctt ccgctctgtg cggttccggc
       61 gtgcatccgc cgcgctcgcg gcatccggcg ctcacccgcg ccatcacaga cgcagacgcc
      121 gctcagcggc atccgaagag aggttttcca tgttccgcat ccgcaccgca ctggcagggg
      181 ccgtcgtggc cgtcgccgcc gtcctggtcg tcgccgcccc ggcatccgct catgaccaga
      241 tcgtgtcgag cacgcccgcc gccgacgagc agctcaccac cgcgcccgac caggtcgtgc
      301 tcacgttctc caacaacctg ctggcgctct ccgacaacag cggcacggcc atgaccgtgg
      361 tcgacgagca gggcaccgac tgggtggcgg gcgcaccgac cgtggccgcg gacaccgtca
      421 ccgtgccgtt gaagccgggc atgcccaacg gcgcgtacga cgtgacgtgg aaggtcgtct
      481 ccagcgacgg ccacccgacg tccggcgagt actcgttctc gatcgcggcg gccgaggagc
      541 cgacgccgag caccgggccc tcggagagcg cacagcccac ggcacccgcg agcacccagg
      601 cacccgccga gtccgcggag acggagtccg cgccctggcc gctgctgatc gggctgggcg
      661 tcgtgctgct gatcgccatc gtggtggtca tcgtgatcgc cgcgcgcaag cgccgcaact
      721 gaccgtcgcc tccggctcgg gaggagaact cgcgctcggg aggggcatcc tgcgggattc
      781 gtcctcccga cgcggagttc tcctcccgag cgcgtgtggc gcatccgagc acgtgaggcg
      841 cgcggaggcg agtcgcccgc ggtgataccg tcaggccggg aggaaccgca tggcgatcat
      901 cgacaacggg atctatgtgg acggggtccg cacggagaac ccggccagcc tggacgagac
      961 gttcgagctg atgcgctccc gcggcgggat gggctggatc ggactgtacc gtcccgacgg
     1021 cgccgagctg gcgtcggtcg cgaacgagtt cggactgcac gagctggcgg tcgaggatgc
     1081 cctggtcggc caccagcggc ccaagatcga gcgctacggc gagcacctgt tcgtcgtgct
     1141 gcgccccgcc cgctatctgg acgccgagga ggaggtggag ttcggcgagg tgcacgtctt
     1201 cgtcggcccc gacttcgtgg tcaccgtgcg tcacgccgaa gcgcccgatc tcgcccgggt
     1261 gcggcatcgc ctcgagtccg agcctgagct gctggctcgc ggaccggagg ccgtgctcta
     1321 tgcgatcctc gatgaggtcg tggaccagta cgcacccgtg ctcgccgggc tcgagaacga
     1381 catcgacgag atcgagagcc agctgttcgt cgaggatgtc gacgcgacgc agcgcatcta
     1441 cgaactgggc cgcgaggtga tcgacttcca gcgcgccgtg cagccgctga cctcgatggt
     1501 cgaggcactg ctgcggggcg gagtgaagta cgccgccgac gaggagctgc agcgctacct
     1561 cagggacgtg cacgaccacg ctatccgcgt cgcagagcgc tccgcgagct tccgctcgat
     1621 cctcgacaac gccctgaccg tggcatccac gatcgtcgcc cgcagacaga acgacgagat
     1681 gaagcggatg acggaggaga gcatccgcca gggcgaccag atgaagagga tctccagctg
     1741 ggcggcgatc ctgttcgcgc ccacgctgat cggcgcgatc tacggcatga acttccgcgt
     1801 gatgcccgaa ctgcactgga cgttcggcta tccgctggcg atcgcggcga tggtcgcatt
     1861 cggaggcggg ctgtacctgg tgttcaagcg caaggggtgg ctgtgagctc gccgccgcgc
     1921 actcgcgccg accagcgcgg cgcagacctg tggttccgcc tgatggggct gccgaccttc
     1981 gtgccgctgc gcagatggtt caccgacctg ccgcggcgag tcgctccgct gatcgcgtcg
     2041 gtgacgatcg cgcggttcct gctgcaggac ctcgtcgacg atgcgatcga ggtcgcggtc
     2101 gagttcttcg gcaccgacga atgggcgatc ttcgcgagcg tgctcgtgct gatcggcgtc
     2161 gcgctggtga tcgcctggac cgcctacgcc ctgctgcggg gactcctgcg ccggctgcga
     2221 cccccggtcg gcgcgaccgt ggcgacggcg gtcatcgtgc tgtgcgtggc agggctgatc
     2281 atcggcgggt acctgatgac accggatgcc acggtgggac cggtgctgtc ggcgctggcg
     2341 ctggtgctgg gctgcgtgct cttcacgggc atgggcggag gcgccctgct cacgtgggcg
     2401 tcgcgtctgg cggtccgcaa cgtctcggcg atcggctaca tggcgtcgat cgcgctcccg
     2461 gtgatcctga tgctcgtggt cttcgcgttc ttctcgggcg aggtgtggca gatggcctcc
     2521 gccctcgggt ggcggtcgat ctggacgctg gccctcgtcg tcggcgccct cgccgtggtg
     2581 gtcgtgctga gtgtgtgcgc caaggagctc gacgaggaca tgcacgtgca gggacatgag
     2641 caccggctgg cgctcgtcgt cggcactccg gccgagggtc ggatgcagga ggacaccctg
     2701 caccgtccgc tgcaccgcgc tcagcggatc aacctgctgc tcgtgatggc ggtggcgcag
     2761 gtgctgcagg cggtcttctt cgccgccctg ctctggatgc tgctggtggt gatcggtgcg
     2821 atcgcgatcc cctacggagt ggtcgagctg tgggtgggcg ccggggcggc atccatgccg
     2881 ctggaggtcg tgcgcatcga ggtggcgtcg gcgacgctgc cgctcacggt gaatctgctg
     2941 aaggccgccg ccctgctctc ggtgatcgcg acgctgccgt tcgtgttctc cgcggtgagc
     3001 gaggcgcgct atcgggagcg cttcttcgat ccgatcatgg ccgacattcg ccgtgcgatc
     3061 gtggtgcgcg acgcactgcg ggcgtgacgg cggcacgcgg gagagcgttc agccgcgcag
     3121 cgcggcggtt gcggcgatcg tcgcgagggc ggtgagcgtc accagcatcc agagcatccc
     3181 gccgagcgcc agcggacgcc agccggcgcg gcgcagcgcc gggatgtcgg tggacaggcc
     3241 gatgccggcc agcgccatcg cgatcaggaa cacgctcgcg tgcacgagca cctggcgcgg
     3301 ggcgtcgggg atcagaccgg tggagttgat cagtgccacc acgacgaatc cgatcaggaa
     3361 ccagggcacg agcttcgcga tgcgccggcc ggtcagcttc tgaccgtccg cgctgcggcg
     3421 ggcctccatg atcgacagac cgatgctgat cgggatgatc atcagggtgc gcacgagctt
     3481 gacgaccacg gcgaagccga gcgccgtggt ggcgaacacg ccggccgctg cgaccaccga
     3541 gctggtgtcg ttgacggcgg tgcccgcgaa caggccgaac gtgtgcgggt cgaggttcag
     3601 tgcgtggccg atgaggggga agatcagcac cgcgaggatg ttgaacagga agatcgtcga
     3661 gacggcgtag gcgatctcag cgctcgccgc gccgatcacg ggcgagacgg cggcgatcgc
     3721 ggatgcgccg cagatgcccg tgccgacgcc gatcagcgtg gtcaggcggc gctcggtgcc
     3781 cagcatccgc cccagcagcc atgcgcccac gaggcacacg atgagggtcg tgagcatgat
     3841 cggcaaagac tccaggccga ccttcgcgat cgcgccgatc gacagctgca cgcccagcac
     3901 aaccacggcg agttgcagca ggaacttgcc ggagtacgcg attccgggct ggatgcgggc
     3961 atccgtcctc tcccgccccg ccatgacccg gggccggacg atcgacagga gcaccccgat
     4021 gatgatggcc ggaaccgccg agccgagcac aggaaggatc gcgccgatgc cggtggagac
     4081 cagggcgatc gcggccgtga ggatgactcc ggggaagacg tcgacgccgc gccggacgag
     4141 tcgggagggg agggtctgaa cgggggagag ctgcaccctt ccatcctgct gacccgccgt
     4201 ccgctggggg taggggctat cgtcggtagg ggtacaggcc aggactgtga gggggccatg
     4261 atcgacgatc ccgacatcgg cgcactgcgg ctgctcgcca cggccgtgcg ggaggggtcc
     4321 atctcggcgg ctgcgcgcga ggcgcgcgtg acgcagcaga ccgcgtcggc gcgactgcga
     4381 g
//
LOCUS       DATMSX010000290         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_99270,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000290 DATMSX010000000
VERSION     DATMSX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99270"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VN041_08790"
     CDS             complement(<1..1121)
                     /locus_tag="VN041_08790"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HWU29174.1"
                     /translation="MTATLTIASVSLPSAPVGEVNPLPAVATMPQAPYEASTEGLPAE
                     IASNIAYGRVDSIHPYLLQDQYGRERRTAPMRVAVLENEHMRAEFALDLGGRLVRLLD
                     KADGRDLVYRNDVFQPANLALRNAWFSGGVEWNIGMRGHWPLTCDPLYAAEVTGPDGE
                     PVLRMWEYERVRGLIVQLDATLDAHAPALHVQVRVRNPHPAETGMYWWTNIAVAQTAA
                     SRVFTSATQAYRTDYDGRLSLVDLVSDDVSYPASAPVAADWFHVLPAGELPWIAALEA
                     DGSGLGHVSTARLKGRKLFVWGDNAGGRHWQDWLGGDYFEIQGGLATTQYEHLRMPGG
                     ATWEWQETFLPVHAGDPSGAWAAASDRVASAVRTAAARAS"
     gene            1285..1536
                     /locus_tag="VN041_08795"
     CDS             1285..1536
                     /locus_tag="VN041_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HWU29175.1"
                     /translation="MPLYMDVHSLGDAVSLDDVAKAHAADLALQDQHGVQYLRYWVDE
                     AGGKIFCLVDAPDPEAANTVHREAHGLVADEIHLVREGA"
     gene            1559..>2115
                     /locus_tag="VN041_08800"
     CDS             1559..>2115
                     /locus_tag="VN041_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWU29176.1"
                     /translation="MPDFDFRALRRFPDVEAPNLQAWDATDQLLVRWALGSGVPGDEI
                     AVIGDEYGAITLALADAGVSGIRVHQDLVTGRRALAHNAAALGVPGWTSHELDAHLLA
                     GVRLVLLQLPKALAELEEIADAVARWAEPDATLVAGGRVKHMTLTQNEVLARRFGVVQ
                     PQRAERKSRLIVASEPLAVPTEPPFP"
BASE COUNT          304 a          767 c          737 g          307 t
ORIGIN      
        1 gacgcccgcg ctgccgccgt gcgcactgcc gaagcgacgc gatcactcgc cgcagcccac
       61 gctccggacg gatcgccggc gtgcacgggt aggaaggtct cctgccactc ccaggtcgca
      121 ccgcccggca tccgcaggtg ctcgtactgc gtggtcgcca gcccgccctg gatctcgaag
      181 tagtcgccgc ccagccagtc ctgccagtgg cggccgcccg cgttgtcacc ccagacgaac
      241 agcttgcgcc ccttcagccg ggcggtcgag acgtgaccga ggccggaccc gtccgcctcc
      301 agtgccgcga tccacggcag ctcgcccgcc gggagcacgt ggaaccagtc ggcggccacg
      361 ggggcggatg ccgggtacga cacgtcgtcg ctgacgaggt cgaccagcga caggcggccg
      421 tcgtagtcgg tgcggtacgc ctgcgtcgcg ctggtgaaca cccgcgacgc cgcggtctgc
      481 gccacggcga tgttcgtcca ccagtacatg cccgtctcag ccgggtgggg gttgcgcacc
      541 cgcacctgca cgtgcagcgc gggagcatgc gcgtccaggg tcgcatcgag ctgcacgatc
      601 agtccgcgga cgcgctcgta ctcccacatc cgcagcaccg gctcgccgtc gggtccggtc
      661 acctcggcgg cgtacagcgg atcacaggtg aggggccagt ggccgcgcat cccgatgttc
      721 cactccacgc cgcccgagaa ccaggcgttg cgcagtgcca ggttcgccgg ctggaacacg
      781 tcgttgcggt agacgaggtc gcgtccgtcc gccttgtcca gcaggcgcac cagccggccg
      841 ccgagatcga gcgcgaactc cgcccgcatg tgctcgttct ccagcacggc cacccgcatc
      901 ggggcggtac ggcgctcgcg accgtactgg tcctgcagca ggtacgggtg gatgctgtcg
      961 acccgtccgt acgcgatgtt cgacgcgatc tcggccggaa gcccctcggt cgacgcctcg
     1021 tacggagcct gcggcatcgt cgccaccgcg ggcagcggat tcacctcgcc gacgggtgcg
     1081 gagggaagcg agaccgaggc gatcgtgagc gttgcagtca tgatgatgct cacgctagcc
     1141 aatgcgcgtg cccgcgccca gcatccggtg caagcggctc tgtcgcggcg gcccgtaccg
     1201 gcgtagcatc gcttctcaca agccgttggg ggcgcacccc gaccaccggc cgaggcggcg
     1261 gcgatcacag atggggattc gatcatgccg ctctacatgg atgtccattc gctcggcgac
     1321 gccgtgagtc tcgacgatgt cgccaaagcg catgccgccg acctggccct gcaggaccag
     1381 cacggcgtgc agtacctgcg ctactgggtc gacgaggcgg gcgggaagat cttctgcctg
     1441 gtcgacgcgc ccgaccccga ggccgccaac acggtgcacc gcgaggcgca cggcctggtc
     1501 gccgacgaga tccacctggt cagggagggc gcctagccgg tgtcggtagg gtgagcgggt
     1561 gcccgacttc gacttccgcg cgctgcgccg attccccgac gtcgaggcgc cgaatctgca
     1621 ggcctgggat gccaccgacc aactgctcgt gcgatgggct ctcggttcgg gcgtcccggg
     1681 cgatgagatc gccgtcatcg gcgacgagta cggcgcgatc acgctcgcgc tggcggatgc
     1741 cggggtgtcc ggcatccgcg tgcatcagga cctggtcacc gggcgcaggg cgctggcgca
     1801 caatgccgcc gcgctgggcg tccccgggtg gacgtcgcac gagctcgatg cccatctgct
     1861 ggccggcgtg cggctggtgc tgctccagct gccgaaggcg ctcgccgagc tcgaggagat
     1921 cgcggatgct gtcgcccgct gggccgagcc cgatgcgacg ctggtcgccg gcggacgcgt
     1981 caagcacatg acgctcacgc agaacgaggt gctcgcgcgc cgcttcggcg tcgtccagcc
     2041 gcagcgcgcg gagcgcaagt cgcggctgat cgtggcatcc gagcccctcg ccgtcccgac
     2101 ggaaccgccc ttccc
//
LOCUS       DATMSX010000291         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_70721,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000291 DATMSX010000000
VERSION     DATMSX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70721"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VN041_08805"
     CDS             complement(<1..739)
                     /locus_tag="VN041_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWU29177.1"
                     /translation="MIPDAAQHVVVHADPTASGVVIGAPGTGKTAALVDRVVHLLDGV
                     LLPEELLVITPSRQAATALRDRVGVRIDQATPGPLVRSLGSFAFQLVRGAMVREGQDP
                     PALLTGADQDRILADLLAGDAEDDERRWPDALSPAVRASKGFRSELRAFLAECTELGV
                     RPGELEGSSREVWQSAADFLAEYRDVMDGMRVAHRDIPELLSEATGILSTADPASLGP
                     LAALRTVLIDDAQELTRGGIRLVRALRE"
     gene            880..1104
                     /locus_tag="VN041_08810"
     CDS             880..1104
                     /locus_tag="VN041_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HWU29178.1"
                     /translation="MEIRIGIVNTGRELSFETSTSADEIRSQVAGALEQNASHLSFTD
                     AKGSSYIVPTANLAYIELGTEESRRVGFVA"
     gene            1106..1393
                     /locus_tag="VN041_08815"
     CDS             1106..1393
                     /locus_tag="VN041_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29179.1"
                     /translation="MYILLALIAACSLGIGLHFVLPKRDLRGVAVTPGIATAAAAAIY
                     TILQWSGVAETSAWLWFASLGGGLLLAALLTVAISAVRTRRDAEAKQALGI"
     gene            complement(1411..2121)
                     /locus_tag="VN041_08820"
     CDS             complement(1411..2121)
                     /locus_tag="VN041_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HWU29180.1"
                     /translation="MVNWFWKRKPQRRTLTLRSRGDDGGAQRVDFAELAPELNRFLGQ
                     AAYLQLGYFETLSRSIRGTQSLARKEALSRAAGAALDKHRGIVKVIAEQGEDPTEVML
                     PFRENLDAFRRKTIGVRQEETLLAVHLTAGMLDDFYLALASSYGRTGEQVAEILRQRE
                     AGDEIVAIIRETIESDHEWRSLLSMWGRRLVGDTLLVCRDALRPGRLEADDSRIEPVY
                     TELMGAHARRMDAMGLAS"
     gene            2307..>3679
                     /locus_tag="VN041_08825"
     CDS             2307..>3679
                     /locus_tag="VN041_08825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU29181.1"
                     /translation="MTSFADLGIDQDILDALAAKGIVDAFPIQEQTIPLGLPGQDIIG
                     QAKTGTGKTFGFGIPVVQRLGQDPEPGVKALIVVPTRELAVQVYEDIDMLTSNRSTSV
                     VAIYGGKAYEGQIDQLKAGAQIVVGTPGRLIDLANQRLLDLSHATEVVLDEADKMLDL
                     GFLADIEKIFSKVPAVRHTQLFSATMPGPIVALARRFMTNPIHIRANDPDEGLTQANI
                     KHIVYRAHSLDKDEVIARILQAEGRGKTVIFTRTKRAAQRLVDELGDRGFNVGGVHGD
                     MGQEQRERSMAAFKAGKRDVLVATDVAARGIDVDDVTHVINHTIPDEEKTYLHRAGRT
                     GRAGKTGIAVTFVDWEDLHKWALINRALEFGQPEPVETYSSSPHLYTDLNIPEGTKGR
                     LRTAPKAEKAPSERRQRQPQKAAEVAAEGTDEGTTRRRRRRRRSSDAEQVGATFAEGA
                     ETSASAP"
BASE COUNT          589 a         1312 c         1267 g          511 t
ORIGIN      
        1 gctcgcgcag cgctcgcacc aggcggatgc caccgcgggt gagctcctgc gcgtcgtcga
       61 tgagcacggt gcgaagggcg gcgagggggc cgagggacgc cggatccgcc gtgctcagga
      121 tgcccgtcgc ctcggacagc agttcgggaa tgtcgcggtg ggcgacgcgc atgccgtcca
      181 tcacgtcgcg gtactcggcg aggaagtccg ccgccgactg ccacacctcg cggctggagc
      241 cctccagctc gccaggccgc acgcccagtt cggtgcactc agccaggaag gcgcgcagct
      301 cggagcggaa gcccttcgac gcacggacgg cgggactgag ggcgtcgggc cagcggcgct
      361 cgtcgtcctc ggcgtcgcct gcgaggaggt cggcgaggat gcggtcctgg tctgcgccgg
      421 tgagcagcgc gggtggatcc tgcccctcgc gcaccatggc gccgcggacg agctggaagg
      481 cgaatgagcc cagcgagcgc accagcggcc cgggcgtcgc ctggtcgatc cgcacgccca
      541 cccggtcgcg cagtgcggtg gccgcctggc ggctcggtgt gatcacgagc agctcctcgg
      601 ggagcagcac gccgtcgagc aggtgcacga cgcggtccac gagcgcggcc gtcttgcccg
      661 tgcccggcgc gccgatcacg acgccggatg ccgtggggtc ggcgtgcacg acgacgtgct
      721 gagcggcatc cgggatcatg tcctccacgc tagcggcggg tgccgacagc gtcggtccat
      781 cgcgctgtgc gcccagggcg aaatcggcgc tgccgccggg ctcggcgcgc gggccagcgg
      841 gtagagtcga ccgtgttcgc agacatctga ggagaacgcg tggaaatccg catcggcatc
      901 gtcaacaccg gtcgtgagct gagcttcgag accagcacca gcgccgacga gattcgctcg
      961 caggtggccg gagcgctcga gcagaacgcc agccacctga gcttcacgga tgcgaagggc
     1021 agctcgtaca tcgtccccac cgcgaacctc gcctacatcg agctgggcac cgaggagtcg
     1081 cgccgcgtcg gcttcgtcgc ctgacatgta catcctgctc gcgctgatcg ccgcctgctc
     1141 gctgggcatc ggactgcact tcgtgctgcc gaagcgcgat ctgcgcgggg tcgcggtgac
     1201 accgggtatc gcgaccgccg ccgctgcggc gatctacacg atcctgcagt ggtcaggcgt
     1261 cgccgagacc agcgcgtggc tgtggttcgc gagcctgggt ggcggactgc tgctggcggc
     1321 ccttctgacc gtcgcgatca gcgcggtgcg cacacgccgc gacgcagagg cgaagcaggc
     1381 gctcggcatc tgacgccgcg agagcggatg ctacgaggcg agtcccatcg catccatgcg
     1441 gcgcgcgtgc gcacccatca gctcggtgta gaccggctcg atgcggctgt cgtcggcctc
     1501 caggcggccg gggcgcagcg cgtccctgca caccagcagc gtgtctccga ccagtcgacg
     1561 cccccacatc gacagcagtg agcgccactc gtggtcgctc tcgatcgtct cgcggatgat
     1621 cgcgacgatc tcgtcgccgg cctcccgctg acgcaggatc tccgcgacct gctccccggt
     1681 acggccgtag ctggaggcca gcgccagata gaagtcgtcc agcattccgg cggtcaggtg
     1741 gaccgcgagc agggtctcct cctggcggac gccgatggtc ttgcggcgga aggcgtcgag
     1801 gttctcccgg aacggcagca tcacctcggt ggggtcctcc ccctgctcgg cgatgacctt
     1861 cacgatcccg cgatgcttgt cgagcgccgc tcccgcggcg cgggacagcg cctccttgcg
     1921 ggccagggac tgcgtgccgc ggatgctgcg gctgagcgtc tcgaagtagc ccagctgcag
     1981 gtaggcggcc tgtccgagga agcgattcag ctcgggcgcg agctcggcga agtccacgcg
     2041 ctgcgcgccg ccgtcgtcgc ccctgctgcg cagggtgagc gtgcgtcgct gcggcttgcg
     2101 cttccagaac cagttgacca cccgcccaga ctaccggccg cggtgccgga gcctgcggag
     2161 gaacggacac gcctccggta ggctgttcct gtcctgccat cggagcagga ccccgcgcct
     2221 gtggcagcag ttgacggcgt ggatcacaac gaggcgccgc tcgcacggca gaccgccgcg
     2281 cacgcgccgg acaggcaaag acagacgtga catcattcgc tgatctcgga atcgatcagg
     2341 acatcctcga cgcactcgcc gcgaagggca tcgtcgacgc gttcccgatc caggagcaga
     2401 ccatccccct cggcctcccc ggccaggaca tcatcggcca ggccaagacg ggcaccggca
     2461 agaccttcgg cttcggcatc ccggtggtgc agcgcctggg tcaggacccg gagcccggcg
     2521 tcaaggcgct gatcgtcgtc cccacccgcg agctcgcggt gcaggtgtac gaggacatcg
     2581 acatgctgac ctcgaaccgc tccaccagcg tggtcgccat ctacggcggc aaggcgtacg
     2641 agggtcagat cgaccagctc aaggccgggg cgcagatcgt cgtcggcacg ccgggtcgtc
     2701 tgatcgacct ggccaaccaa cgcctgctcg acctgtcgca cgcgaccgag gtcgtgctcg
     2761 acgaggccga caagatgctc gacctgggct tcctggccga catcgagaag atcttctcca
     2821 aggtgccggc cgtgcgacac acccagctgt tctcggcgac catgcccggc ccgatcgtgg
     2881 cactggcccg ccggttcatg acgaacccca tccacatccg cgcgaacgac cccgacgagg
     2941 gcctgacgca ggcgaacatc aagcacatcg tctaccgcgc ccactcgctg gacaaggacg
     3001 aggtcatcgc ccgcatcctg caggccgaag gccgtggcaa gacggtgatc ttcacccgca
     3061 ccaagcgcgc cgcgcagcgt ctggtcgacg agctgggcga ccgcggtttc aacgtcggcg
     3121 gcgtgcacgg tgacatgggc caggagcagc gcgagcgttc gatggcggcg ttcaaggccg
     3181 gcaagcgcga tgtgctggtc gccaccgacg tcgccgcccg cggcatcgac gtcgacgacg
     3241 tgactcacgt catcaaccac acgatccccg acgaggagaa gacgtacctg caccgcgccg
     3301 gccgcaccgg ccgcgcaggc aagacgggca tcgcggtcac cttcgtcgac tgggaggacc
     3361 tgcacaagtg ggccctgatc aaccgggcgc tggagttcgg tcagcccgag cccgtcgaga
     3421 cctactcgtc gagcccgcac ctctacaccg acctgaacat ccccgagggc accaagggcc
     3481 gcctgcgcac cgctccgaag gccgagaagg ctccgtcgga gcgtcgccag cgtcagccgc
     3541 agaaggccgc ggaggtcgct gccgagggca cggatgaggg caccacgcgt cgtcgccgcc
     3601 gccgtcgtcg cagctcggac gcagagcagg tcggcgcgac cttcgccgaa ggcgccgaga
     3661 cctcagcatc cgcccccag
//
LOCUS       DATMSX010000292         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_137031,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000292 DATMSX010000000
VERSION     DATMSX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137031"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1731)
                     /locus_tag="VN041_08830"
     CDS             complement(58..1731)
                     /locus_tag="VN041_08830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWU29182.1"
                     /translation="MLRGPVAVLAGAGTGKTRVITNRIAHGVDTGAYSPSRVMAVTFT
                     AKAAGELRGRLRALGVGGVSARTFHSAALAQLNFFWPTLTGDTAPSIIDNKVRMLGQA
                     ADAMRLRPSTATLRDIASEIEWRKVSMLSIEQYAQQGRPITGIRPEQLLELQAAYEQL
                     KDDRRQLDFEDVLLACAGMLETESRVAAAVHEQYRHFTVDEYQDVSPLQNRLLELWLG
                     DRRDICVVGDASQTIYSFAGAQQRYLLEFEKRHPDATVVRLETNYRSQAPILEAANAL
                     MHGRPGALALVPAREDARAETPTVTAYDTEREEADGIAAAIADRIASGVSPSDIAVLY
                     RAHVQSAALQQALAARGIATSVLGGTRFFDMPEVRQAVLGLRGAAVAPTDAGFLPTVR
                     DVLRGLGLTDEPPAASGAQRDGWEARRAILRLAEEAPKGTTLRVFADELMARAKDQHE
                     PTLHTVTLSTLHAAKGLEWPHVLLAGWAEGALPISYASTFEAVDEERRLAYVGITRAA
                     RTLAISWSRSAGRGERAPSRFLAEIGTASRGTGILRDGAPSARRAARRD"
BASE COUNT          233 a          607 c          635 g          257 t
ORIGIN      
        1 ggctggcagc gcgcgtgctg cgcacgcctg tcgaggaggc cgggctgacg gtgcggatca
       61 gtcccgacgg gcggctcgcc tggcgctcgg tgcgccatca cgaagaatgc ccgtgcctcg
      121 agacgccgtc ccgatctccg cgaggaaccg cgacggcgcc cgctcccctc gtcccgcgct
      181 gcgagaccac gagatcgcga gggtacgcgc ggcccgcgtg atgcccacgt aggccaggcg
      241 gcgctcctcg tcgaccgcct cgaacgtcga tgcgtaggag atcggcagag cgccctcggc
      301 ccagcccgcc agcagcacat gcggccactc caggcccttc gcggcgtgca gggtcgacag
      361 cgtcacggtg tgcagggtcg gctcgtgctg gtccttcgcc cgcgccatca gctcgtcggc
      421 gaacacccgc agcgtggtgc ccttcggtgc ctcctcggcg agacggagga tcgcccggcg
      481 cgcctcccac ccatctcgct gcgcaccgct ggctgccggc ggctcgtcgg tgagtcccag
      541 gccgcgcagc acgtcgcgca cggtgggcag gaacccggca tccgtgggcg cgacggctgc
      601 gcctcgcagt ccgagcactg cctgacgcac ctcgggcatg tcgaagaagc gggttcctcc
      661 gagcacgctc gtggcgatgc cgcgggcggc gagcgcctgc tgcagcgcgg cggactgcac
      721 gtgcgcacgg tagagcacgg cgatgtcgga cggcgagacg ccagaggcga tgcgatcggc
      781 gatggccgca gcgatgccgt cggcctcctc gcgctcggtg tcgtaggccg tcacggtggg
      841 cgtctccgca cgcgcgtcct cccgcgcggg cacgagggcg agcgctccgg gtcgcccgtg
      901 catgagcgcg ttcgccgcct caaggatggg cgcctgggag cggtagttgg tctccaggcg
      961 cacgaccgtg gcatccggat ggcgcttctc gaactccagc aggtagcgct gctgcgctcc
     1021 agcgaaggag tagatggtct ggctcgcatc gccgaccacg cagatgtcgc gacgatctcc
     1081 gagccacagc tccagcagcc ggttctgcag cggcgagacg tcctggtact cgtcgactgt
     1141 gaagtgccgg tactgctcgt gcacggccgc ggcgacgcgg gactcggtct cgagcatgcc
     1201 cgcgcatgcg agcagcacgt cctcgaagtc gagctggcgg cggtcgtcct tgagctgctc
     1261 gtaggcggcc tgcagctcga gcagctgctc cgggcggatg ccggtgatgg gtcgtccctg
     1321 ctgcgcgtac tgctcgatgg agagcatcga caccttgcgc cactcgatct cggaggcgat
     1381 gtcgcgcagg gtcgcggtgc tcggacgcag gcgcatggca tcggctgcct ggcccagcat
     1441 ccgcaccttg ttgtcgatga tcgagggcgc ggtgtcgccg gtgagcgtcg gccagaagaa
     1501 gttcagctgc gcgagcgcgg cgctgtggaa cgtacgggcc gagacgccgc cgactccgag
     1561 cgcgcggagt cggccgcgca gctctcctgc ggccttcgcg gtgaaggtca cggccatgac
     1621 gcgcgacggc gagtacgctc ccgtgtcgac gccgtgcgcg atgcggttcg tgatcacccg
     1681 ggtcttcccg gtgccggcgc cggcgagcac ggcgacgggt ccgcgcagca ag
//
LOCUS       DATMSX010000293         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_146525,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000293 DATMSX010000000
VERSION     DATMSX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146525"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VN041_08835"
     CDS             complement(<1..198)
                     /locus_tag="VN041_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29183.1"
                     /translation="MPQTPFRRIGAGTVAAVITAVLCTSALPLAASPAFAQDAVPTPV
                     TTPGATESPAPTETPTPTETPD"
     gene            complement(385..681)
                     /locus_tag="VN041_08840"
     CDS             complement(385..681)
                     /locus_tag="VN041_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29184.1"
                     /translation="MDADNDTSISGREARVNAEFQKRQTQLFTRFALAEASVLALAVL
                     AIFVLKLVEPETGILILLGIAVLSGMVLTGLLMRTIKAKQQALAQARGEAGSIW"
     gene            complement(846..1904)
                     /locus_tag="VN041_08845"
     CDS             complement(846..1904)
                     /locus_tag="VN041_08845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU29185.1"
                     /translation="MKAFGFLSFGHYADVPGSVTRTAGDMLKQTIEIAEGADEIGVNG
                     AYVRVHHWARQAASPIPLLTAMAARTERIEVGTGVIDMRYENPFQFAEEAAALDYIAD
                     GRIALGVSRGSPETALRGYEVFGFHDPEDPERGSVMAREKFELFLRAIDGELIAPGDP
                     RMVGAGHYLAIEPHSPTLRDHIWWGAGSRATAETTGRMGLNMMSSTLLTEATGQPFHE
                     LQREQIDLFRAAYKEAGHTGSPRVSVSRSVFPLVSDKDRAYFGLRSEESNDQIGIIDG
                     MRSTFGKTYAAEPDVLIEQLRADEAVMAADTLMLTIPNQLGPEYNLHVLQAFAEHVAP
                     ALGWKPNTEGPVQGDPIA"
     gene            complement(1933..3237)
                     /locus_tag="VN041_08850"
     CDS             complement(1933..3237)
                     /locus_tag="VN041_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWU29186.1"
                     /translation="MPQPHTAVEWAVPRVGVDGLEALGLAVADGADVRPLAVIRPQSF
                     VDADGVGEWWIASDLDEIALGTSLPADHVLGVGGASRTLAELVIPTPVERALDLGTGC
                     GIQALLVSRRAQHVVATDISARALAYAELNAQLNGVRNVELRLGSMFEPVAGEAFDLI
                     VSNPPFVITPRVSGVPEYEYRDGGLVGDALVQQFLQTAPAHLTPGGVAQLLGNWESRA
                     GEDGLERVRSWIGEQLDAWVIQREQLSPLSYAELWIRDGGTLPRDSGFTPLLTAWLDD
                     FAARDVTAIGFGYILVRHPSAPASVEGSAPLRRFETITQPVSNLGRALGAGLAAHDVL
                     ADGIPDRLVVASDVTEARHHMPGNDDPSVIELRQGGGFARTISVDSALAGFVGACDGE
                     LTVMQIVQALADIFEVPLTDLWADLEPRIRRLVEDGLLLPAE"
     gene            3223..>3492
                     /locus_tag="VN041_08855"
     CDS             3223..>3492
                     /locus_tag="VN041_08855"
                     /inference="COORDINATES: protein motif:HMM:NF039257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tectonin domain-containing protein"
                     /protein_id="HWU29187.1"
                     /translation="MRLRHPEHPQPAQRRQRIVARNADGRLALFGTNSADNIWHRAQT
                     SIGGAWNAWTQFDGALRSIAAETNADGRIEVFGVNAAGQIWHRSQV"
BASE COUNT          557 a         1188 c         1247 g          500 t
ORIGIN      
        1 atccggggtc tcggtgggtg tcggggtctc cgtgggcgcc ggggactccg tcgcgccggg
       61 cgtcgtgacc ggggtcggga cggcatcctg ggcgaacgcg ggcgaggcgg cgagcggcag
      121 tgcggacgta cacagcactg cggtgatcac cgcggcgacc gtgcccgcgc cgatccttct
      181 gaagggcgtt tgaggcatga cgggagaacc tgttccgtgg ggggagacgg cagaggagcc
      241 gctcccttta tagggatccc aggtggctca caggttgcgg gatgaggacg agcgcgcgtc
      301 acaggtgaac gtctgcggaa gacggggaga cgtgcggcgt gtcaccgggc cgcacattcg
      361 aggagggcga gtcgggtcag cggctcacca gatgctgccg gcctcgcctc gggcctgggc
      421 gagcgcctgc tgcttggcct tgatcgtgcg catcagcaga ccggtcagca ccatgccgct
      481 cagaacggcg atgcccagca ggatgaggat gccggtctcg ggctcgacga gcttcagcac
      541 gaagatcgcg agcacggcga gcgcgagcac cgacgcctca gcgagcgcga agcgcgtgaa
      601 cagctgcgtc tggcgcttct ggaactcggc gttgacgcgg gcctcccggc ctgagatcga
      661 ggtgtcgttg tcagcatcca tgcccagaag catcgcatga aggcggccgg gcggccggga
      721 acagcgtcac ggcggcccac ttctgaagga cgttcgacgt tctgaaggat gctgaggcct
      781 tcagcatcct tcagaagcgg aatcagcctt cagaaggcgg ggcgccggac gcgcagcggc
      841 gctgctcagg cgatcggatc gccctgcacg ggtccctccg tgttgggctt ccagcccaag
      901 gcgggcgcga cgtgctcggc gaaggcctgc agcacgtgca ggttgtactc cgggcccagt
      961 tggttcggga tggtgagcat gagcgtgtcg gcggccatca cggcctcgtc ggcccggagc
     1021 tgctcgatca gcacgtccgg ctcggccgcg taggtcttgc cgaacgtgga gcgcatgccg
     1081 tcgatgatgc cgatctggtc gttcgactcc tccgaccgca ggccgaagta ggcgcggtcc
     1141 ttgtcgctga ccagcgggaa gacgctgcgg ctgacagaga cgcgcgggct gccggtgtgc
     1201 ccggcctcct tgtaggcagc gcggaagagg tcgatctgct cgcgctgcag ctcgtggaac
     1261 ggctggccgg tcgcctcggt gagcagcgtg gagctcatca tgttcaggcc catgcgtccg
     1321 gtggtctcgg ccgtcgcacg ggatccggcg ccccaccaga tgtggtcgcg cagcgtgggc
     1381 gagtgcggtt cgatcgccag gtagtggccg gcaccgacca tgcgcggatc gccgggagcg
     1441 atcagctcgc cgtcgatcgc gcgcagaaac agctcgaatt tctcgcgggc catcacgctg
     1501 cctcgctccg ggtcctccgg gtcgtggaac ccgaacacct cgtagccgcg cagggcggtc
     1561 tcgggtgacc cgcggctcac gccgagcgcg atgcggccgt cggcgatgta gtcgagcgcc
     1621 gccgcctcct cggcgaactg gaacgggttc tcgtaccgca tgtcgatcac gccggtgccg
     1681 acctcgatgc gctcggtgcg cgcggccatg gcggtcagca gggggatcgg ggatgccgcc
     1741 tgccgcgccc agtggtgcac gcgcacgtag gcgccgttca cgccgatctc atcggcgccc
     1801 tcggcgatct cgatggtctg cttgagcatg tcgccggcag tgcgcgtgac ggagccgggc
     1861 acgtctgcgt agtgcccgaa ggagaggaac ccgaaagcct tcatgctgca tgcaaccgca
     1921 tatatcctgc ggttattccg cgggcaggag caggccgtcc tcgacgaggc ggcggatgcg
     1981 gggttcgaga tcggcccaga gatcggtcag cggcacctcg aagatgtccg ccagcgcctg
     2041 cacgatctgc atcacggtga gctcgccgtc gcaggcgccg acgaaacccg cgagcgcgga
     2101 gtcgacgctg atcgtgcggg cgaaaccgcc gccctgccgc agctcgatca cactcggatc
     2161 gtcgttgccg ggcatgtggt ggcgggcctc ggtgacatcg gatgcgacga ccaggcgatc
     2221 cgggatgccg tcggcgagca cgtcatgcgc ggccagaccc gcgcccagag cccggcccag
     2281 gttcgagacc ggctgcgtga tggtctcgaa acggcggagc ggagcggatc cctcgacgga
     2341 cgcaggcgcc gaaggatgcc gcaccaggat gtagccgaat ccgatggcgg tcacatcgcg
     2401 cgccgcgaag tcgtcgagcc aggcggtcag gagcggggtg aagccggaat cgcgcggcag
     2461 cgtcccgcca tcgcggatcc acagctcggc gtacgagagc gggctgagct gctcgcgctg
     2521 gatcacccag gcatccagtt gctcgcctat ccaggagcgc acccgctcga ggccgtcctc
     2581 gccggcgcgc gactcccagt tgccgagcag ctgcgccacg ccacccggcg tgaggtgagc
     2641 gggcgcggtc tgcaggaact gctggacgag cgcatccccg accagcccgc cgtcccggta
     2701 ctcgtactcc ggaaccccgg agacgcgcgg ggtgatcacg aacggcgggt tggagacgat
     2761 cagatcgaac gcctcccccg ccaccggctc gaacatgctg cccagacgca gctccacgtt
     2821 gcgcacgccg ttcagctgcg cgttcagctc ggcatacgcc agcgcccgcg ccgagatgtc
     2881 ggtcgcgacg acgtgctgcg cacgccgcga caccagcagg gcctggatgc cgcatcccgt
     2941 gccgaggtcc agggcgcgct ccaccggcgt ggggatgacg agctccgcca gcgtgcggga
     3001 agccccaccc acgccgagca cgtggtcagc gggaagcgag gtgcccaggg cgatctcgtc
     3061 gaggtcgctc gcgatccacc actcgccgac accgtcggca tccacgaacg actgcggacg
     3121 gatgacggcg agcggccgca catcggcacc atcggccacc gccagtccca gcgcttcgag
     3181 cccgtcgacg cccacgcgcg gcaccgccca ttcgacagcg gtgtgcggct gcggcatccc
     3241 gagcacccac agcctgccca gcgtcgccag cggatcgtcg cgagaaacgc ggacggcaga
     3301 ctcgcgctct tcggcaccaa cagcgccgac aacatctggc accgcgccca gaccagcatc
     3361 ggcggcgcct ggaacgcctg gacccagttc gacggcgccc tgcgctccat cgccgcggaa
     3421 acgaacgcgg acggccgcat cgaggtcttc ggcgtcaacg ccgccggcca gatctggcac
     3481 cgctcccagg tc
//
LOCUS       DATMSX010000294         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_184423,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000294 DATMSX010000000
VERSION     DATMSX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184423"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..939)
                     /locus_tag="VN041_08860"
     CDS             complement(397..939)
                     /locus_tag="VN041_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWU29188.1"
                     /translation="MTDSTPTASDEAAQNAALQKAVIVGIQPGQPPHVLAEALRYAKL
                     LQSPLVVVHVDVTRFVTYEDPDGYVHSAPIDLNLDAGAAEFEDVQKMAAEALDKEDIT
                     WTTRQLIGDPALAIKQLADKLDAQLIVIGTRKRGIGESIREFFTGSVAARLAHRQHRS
                     VLVVPQTESVPDDQKDVWDE"
     gene            1092..1280
                     /locus_tag="VN041_08865"
     CDS             1092..1280
                     /locus_tag="VN041_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HWU29189.1"
                     /translation="MGRGRQKAKHTKIARELKSYSPNVNFSALENELGHPSSEEDQYV
                     DKWADQYEDEDEDELERA"
     gene            1376..2200
                     /locus_tag="VN041_08870"
     CDS             1376..2200
                     /locus_tag="VN041_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWU29190.1"
                     /translation="MPRVAALYRHPIKGFTPEQCDELTIQPDGRVAGDRVLAFRFADA
                     TEPEQRDGLDYWPKSKGLALESFPSLATLRVAFDHETQHVRITHDDSLLVDVVLDDEG
                     RAELVEIVTAFVLDSPEWKLLQREGRLPLALVGDGERSRFQDRARGFVSVHSEASVDA
                     LSAALGQTVDDRRFRSNVVIEGADAWDELAWAGEVRIGDLRFTAEGPIVRCLATHANP
                     DTGIRDAKVLTTLTRGVGLPEPTLGRLLLLNGVTGSVDDAGGAGGVIRVGDEVTIG"
     gene            complement(2245..>2931)
                     /locus_tag="VN041_08875"
     CDS             complement(2245..>2931)
                     /locus_tag="VN041_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29191.1"
                     /translation="IAEAVPEGPDFAATGPIATSPSSRRLFARLARIPLVWALALTFG
                     TSSIMAYVAFAWLPVIMVEVAHVSASTAGLLLSLFAFVGLPSSLLIPVLVVRFHATRP
                     LFLVGAIGGMIGILGLLLAPLPGLLWLWTVLFGLVGLLFPLALVLISIRSRTPESAVL
                     LSSFVQSVGYVLAAIFPVLVGVLHDATGGWTVPLLVVGAMLVASIPAGMIAGRRRTVE
                     DEWERRNGAW"
BASE COUNT          485 a          972 c         1042 g          432 t
ORIGIN      
        1 aaaaatggtc gccgggcggc ctctctgctc gggctcgccc tgctgccgct gatgagcggc
       61 tgccttttcg tgacggatac gacccggatg gattccgggc ggcatccact gcgatctggg
      121 cgtggtctcg gcgggcctcg cgcgttcggg tcgcgattgg tcccggatac cgctggccgc
      181 gtccgttcag gtcgcgattc attccgctat ccgggcacga aagcgcgacg aactgcgacc
      241 cggactcacg accggatgcc gtagcgcgac gaactgcgac ccggaggcat atccggctgg
      301 acgcgatccg actcggagac gccgatgcga tccgccgcct ccgcgtgcgg aaacctcgag
      361 cgcgccgcgg agggcgatga gctcgactga ccggggctac tcgtcccaga cgtccttctg
      421 atcgtcgggg acggactcgg tctgcggcac gacgagcacc gagcggtgct gacggtgcgc
      481 cagtcgagct gcgaccgagc cggtgaagaa ctcccggatc gactccccga tgccgcgctt
      541 gcgcgtgccg atcacgatca gctgcgcgtc gagcttgtcc gccagctgct tgatcgccag
      601 ggccgggtcg ccgatgagct gccgcgtggt ccaggtgatg tcctccttgt cgagggcttc
      661 ggcggccatc ttctgcacat cctcgaactc ggcagcgccc gcatcgaggt tgaggtcgat
      721 gggcgcggag tgcacatagc cgtccggatc ctcgtaggtg acgaagcgcg tgacgtcgac
      781 atggacgacc acgagaggcg attggagcag cttggcgtag cggagcgcct ccgcgagcac
      841 gtgcggcggc tgaccgggct ggatgccgac gatgacggcc ttctgcagcg cggcgttctg
      901 agcggcttcg tcggatgctg tgggcgtgga atctgtcatg atgatcgttc ccctcaccgg
      961 ccgcaggacg gcctcaggct ctggcgtgcg tgctatcctg aatgctactc ttaccggcct
     1021 cgagccggtg ccgtgaatac catcgggcgt cgcacagccc gctgctgaac tcgtaaaagg
     1081 gggtctcgcg catgggccgt ggccgtcaga aggcgaagca caccaagatc gctcgcgaac
     1141 tcaagtcgta cagtccgaac gtgaacttct ccgcgctgga gaacgagctc gggcacccct
     1201 cttccgagga ggaccagtac gtcgacaagt gggccgacca gtacgaagac gaggacgagg
     1261 acgagctgga gcgggcctga tcccctccct cgcacgacca gacgccctgg cgaccggcat
     1321 ccgccgagtc gaccagggtt tcgtcgtacc tgtcagccga tgacacaatg gccggatgcc
     1381 ccgcgtcgca gccctgtacc gtcatccgat caaggggttc acgcccgagc agtgcgatga
     1441 gctgaccatc cagcccgacg gccgcgtcgc cggcgaccgg gtgcttgcgt tccgcttcgc
     1501 ggacgccacg gagccggaac agcgcgacgg cctggactac tggcctaagt cgaagggcct
     1561 ggcgctggag tcgttcccct cgctggccac cctgcgcgtg gctttcgacc atgagacgca
     1621 gcacgtgcgg atcacgcacg acgactccct gctcgtcgac gtggtcctgg atgatgaggg
     1681 tcgcgcggag ctcgtcgaga tcgtgaccgc attcgtgctc gactccccgg agtggaagct
     1741 gctgcagcgc gagggccgcc tgccgctggc gctcgtcggt gacggcgagc gctcccggtt
     1801 ccaggaccgg gcgcgcggtt tcgtctccgt gcacagcgag gcgagcgtcg acgcgctgag
     1861 cgccgcgctg ggtcagacgg tggacgaccg ccggttccgc tcgaacgtcg tcatcgaggg
     1921 ggcggatgcc tgggatgaac tggcatgggc cggcgaggtg cgcatcggag atctgcgttt
     1981 caccgcggag ggaccgatcg tgcgatgcct ggcgacacac gcgaaccccg acacgggcat
     2041 ccgggatgcc aaggtgctga cgacgctgac ccgcggggtc gggctgcccg agccgacgct
     2101 cggccgtctg ctgctcctga acggcgtgac cggctcggtc gacgacgccg gcggcgccgg
     2161 cggtgtgatc cgcgtcggcg acgaggtgac gatcggctga cccgtcaggt caggtggcga
     2221 tgtgcggtcc gcgaccgccg gcgatcacca cgccccgttg cggcgctccc attcgtcctc
     2281 cacggtgcgg cggcgaccgg cgatcattcc ggccggaatg ctcgcgacga gcatggcgcc
     2341 gacgaccagc agcggaaccg tccagccgcc ggtcgcgtcg tgcagcacgc cgacaaggac
     2401 ggggaagatc gcggcgagca cgtagccgac gctctgcacg aagctcgaga gcagcacggc
     2461 gctctccggc gtgcgggagc ggatgctgat cagcaccagc gccagcggga acagcagtcc
     2521 gaccaggccg aacagcaccg tccacagcca cagcagtccg ggcagcgggg cgagcagcag
     2581 accgaggatg ccgatcatgc cgccgatcgc gccgacgagg aacagcggcc tggtggcgtg
     2641 gaaccgcacc acgagcacgg ggatgagcag cgaggagggc aggcccacga acgcgaacag
     2701 cgacagcagc agacccgcgg tcgaggcgct gacgtgcgcg acctcgacca tgatcaccgg
     2761 gagccaggcg aaggccacgt aggccatgat cgacgaggtc ccgaaggtga gcgcgagcgc
     2821 ccacaccagc gggatgcgtg cgagccgggc gaacaggcga cgactgctgg gtgaggtcgc
     2881 gatcggcccg gtggcggcga agtcggggcc ttcgggcacg gcctccgcga t
//
LOCUS       DATMSX010000295         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_184454,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000295 DATMSX010000000
VERSION     DATMSX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184454"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2423)
                     /gene="katG"
                     /locus_tag="VN041_08880"
     CDS             complement(144..2423)
                     /gene="katG"
                     /locus_tag="VN041_08880"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HWU29192.1"
                     /translation="MTDVRDDMPGIGENAADIDQTKTVTEEPVEPADAGACPVIHAQP
                     HPTMGSANRVWWPEQLNIKVLAKNGEQRNPLGADFDYKAAFEALDLDQVKKDIVEVLT
                     TSQDWWPADFGHYGPLMVRMAWHSAGTYRVTDGRGGGGTGQQRFAPLNSWPDNVGLDK
                     ARRLLWPIKKKYGQALSWGDLMILAGNAALEDMGFKTFGFAGGRIDAWEPDDDVYWGP
                     ETTWLGDERYSGDRDLEKPLAAVQMGLIYVNPEGPNGNPDPLASARDIRETFARMAMD
                     DEETVALIAGGHTFGKTHGAASDSNLEDNPEAAGLEMQGLGWKNNNGTGKGDDQITSG
                     LEVTWTYHPTRWDNEFFHILYAYEWELFKSPAGANQWRPINGGGADMVPLAHSGGRRE
                     PRMLTSDLALRFDPEYGKISQRFKDDPEAFADAFARAWFKLTHRDMGPRSRYVGAEVP
                     AEELIWQDNVPAVDHALIGAEDAAALKQRVLASGLSVSDLVAFTWAAAASFRGSDKRG
                     GINGGRIRLAPQKDWEANNPARVASITAVLEGIQAEFNDARTDDVRVSFADLVVLAGN
                     AAVEKAAADAGVQVEVVFHPGRTDATQEQTDVASFAFLEPKADGFRNYVSRDTAAIPA
                     EHLLLDKANLLTLTAPEMTVLVGGLRALGANWDGSEYGVFTDRPGVLSNDVFVNLLDL
                     GTTWKPLDPGSHAFEGRKDGSDELVGRGTRVDLLFGSNSELRALAEVYASDDATEKFV
                     RDFAAAWGKVTELDRFDLV"
     gene            complement(2420..2896)
                     /locus_tag="VN041_08885"
     CDS             complement(2420..2896)
                     /locus_tag="VN041_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWU29193.1"
                     /translation="MREELMTDAAEAAIRAAGLRVTDSRRAVYDALLAHPHATADAVH
                     DTVRDVVASTSLQSVYNALGDFAAAGLVRRIEPAGHPMMFELRVDDNHHHLICTSCGA
                     VTDVDCATGHAPCLIPDDAHGYRIAVAEVTYWGLCASCAALQTSIPEEPTPTGRTS"
     gene            3041..3799
                     /gene="aqpZ"
                     /locus_tag="VN041_08890"
     CDS             3041..3799
                     /gene="aqpZ"
                     /locus_tag="VN041_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HWU29194.1"
                     /translation="MSESVSTTEKPSLGAKLAAEGFGTFLLVFGGVGTALFASNHFTD
                     PGANSAVYVAVALAFGLTVLVGVYAFGPISGGHFNPAVTVGAAAAGRIPWRDVAPYII
                     AQVVGGVIASTALVLIGLFGPDKWLQTAQDAGFASNGWDSHSPGGFGMMSAIIIEVIL
                     TGIFVMVILGSTHATRGTANAGIAIGLTLTLIHLISIPVDNTSVNPARSIAAAIYGGG
                     ESLAQLWVFLVFPIVGALIVGYAYKALFDGTTRA"
     gene            complement(3884..4873)
                     /locus_tag="VN041_08895"
     CDS             complement(3884..4873)
                     /locus_tag="VN041_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HWU29195.1"
                     /translation="MDADALTDMRRSEWERLDALSRKRRLTGTEVDELITRYRAASAD
                     LAELKTSVGDSPQGAYLSTILAAARLRFTGASDNILAQTSRFFRLQLPAALYRLRWTT
                     AGVAVVFVLIVLISGAWISSDPARIASLGSPEVLKQYAEHDFVDYYEPMASFAGQVWS
                     NNAWIALQCVLFGVTGVWPVWVIVQNAMGLGVAAAVMASHDRLDVMMLHILPHGMLEL
                     TAIFIAAAAGLHIFWSWAVPGRRTRGESLATGGRSLATVAMGLVFVLLLSGLVEGFVT
                     GSALPWPVKIGIGAVALGIVLFYMLVTGRRAARDGETGDLIEYESGTPTLFAG"
     gene            4890..>5371
                     /locus_tag="VN041_08900"
     CDS             4890..>5371
                     /locus_tag="VN041_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWU29196.1"
                     /translation="MSLPITDEQEILSGEAVAIDVQPIGFVLRAAGALIDMVIGFAVF
                     AAFEILQFWLLSLNLLDLHTLPILSIWAAVISFLVLPITVEMAMRGRSVGKLAVGGRI
                     VRVDGGAISFRHTFIRALVGVLEIYMTLGGGAVIAGAVTARSQRLGDLVAGTYSQRVR
                     T"
BASE COUNT          787 a         1841 c         1878 g          865 t
ORIGIN      
        1 atcgccgacc gctacctcga gctcaaggcc gcgggccgcc tgtagccggc atccgcggcg
       61 cgtcacgcgt ccaggcacgc cggacacgga agacgggaac ggcccccgcc ccagaggggc
      121 gagggccgtt ccgtgagaga cgctcagacg aggtcgaagc ggtccagctc cgtgaccttg
      181 ccccacgcgg cggcgaagtc gcgcacgaac ttctcggtgg catcatccga ggcgtacacc
      241 tcggcgagcg cacgcagctc ggagttcgag ccgaacagca ggtcgacgcg cgtgccgcgg
      301 cccacgagct cgtcggagcc gtccttgcgg ccctcgaacg cgtgggagcc cgggtccagc
      361 ggcttccagg tggtgccgag gtcgagcagg ttcacgaaca cgtcgttgct gagcacgccg
      421 gggcggtcgg tgaacacgcc gtactccgag ccgtcccagt tcgcgccgag agcgcgcagt
      481 ccgccgacga ggacggtcat ctcgggcgcg gtcagcgtga gcaggttcgc cttgtcgaga
      541 agcaggtgct cggcggggat ggcggcggtg tcgcgggaca cgtagttgcg gaacccgtcg
      601 gccttcggct cgaggaaggc gaacgacgcg acgtcggtct gctcctgggt ggcgtcggtg
      661 cgaccggggt ggaagaccac ctcgacctgc acgccggcgt cggcggcggc cttctcgacg
      721 gccgcgttgc ccgcgagcac gacgaggtcg gcgaacgaga cacgcacgtc gtcggtgcgc
      781 gcatcgttga actcggcctg gatgccctcc aggacggcgg tgatgctggc cacccgtgcc
      841 gggttgttcg cctcccagtc cttctgaggc gccaggcgga tccgcccgcc gttgatgccg
      901 ccgcgcttgt cgctgccacg gaacgacgcg gccgccgccc aggtgaaggc cacgaggtcg
      961 gagacgctca ggcccgacgc gagcacgcgc tgcttgaggg cagcggcgtc ctccgcgccg
     1021 atcagcgcgt ggtccacggc cggtacgttg tcctgccaga tgagctcctc cgcgggcacc
     1081 tctgcgccga cgtagcggct gcgcggcccc atgtcgcggt gggtgagctt gaaccaggcg
     1141 cgcgcgaagg cgtcggcgaa cgcctcgggg tcgtccttga agcgctgcga gatcttgccg
     1201 tactccgggt cgaagcgcag cgcgaggtcg ctggtgagca tgcgcggctc acgacggccg
     1261 ccggagtgcg ccagcggcac catgtccgcg ccaccgccgt tgatcggacg ccactggttg
     1321 gcaccggccg ggctcttgaa cagctcccac tcgtaggcgt acaggatgtg gaagaactcg
     1381 ttgtcccagc gcgtcgggtg gtaggtccag gtgacctcca ggccggaggt gatctggtcg
     1441 tcgcccttgc cggtgccgtt gttgttcttc cagcccaggc cctgcatctc caggcccgcc
     1501 gcctcggggt tgtcctcgag gttggagtcg gatgccgcac cgtgggtctt gccgaaggtg
     1561 tggccaccgg cgatcagcgc gacggtctcc tcgtcatcca tcgccatgcg ggcgaaggtc
     1621 tcccggatgt cgcgggcgga cgccagcggg tcggggttcc cgttcgggcc ctcggggttc
     1681 acgtagatga gccccatctg cacggcggcg agcggcttct cgaggtcgcg gtcgccggag
     1741 tagcgctcgt cacccagcca ggtggtctcc gggccccagt acacgtcgtc gtccggctcc
     1801 caggcgtcga tgcgaccacc cgcgaatccg aaggtcttga agcccatgtc ctcgagcgcg
     1861 gcgttgccgg cgaggatcat caggtcgccc caggagagcg cctggccgta cttcttcttg
     1921 atcggccaga gcaggcggcg cgccttgtcg aggccgacgt tgtccggcca gctgttcagc
     1981 ggtgcgaagc gctgctgccc ggtgccgccg ccaccgcggc cgtcggtcac gcggtacgtg
     2041 cccgcgctgt gccaggccat ccggaccatc agcggcccgt agtggccgaa gtcagcgggc
     2101 caccagtcct gcgaagtggt gagcacctcg acgatgtcct tcttgacctg gtcgaggtcg
     2161 agcgcctcga aggccgcctt gtagtcgaag tcggcgccga gcgggttgcg ctgctcgccg
     2221 ttcttggcga gcaccttgat gttgagctgc tcgggccacc acacgcggtt ggccgaaccc
     2281 atcgtggggt gcggctgagc gtggatgacc gggcacgcac cggcgtccgc cggctcgacg
     2341 ggctcctcgg tgacggtctt cgtctggtcg atgtcggcag cgttttctcc gatgccgggc
     2401 atgtcgtcgc gaacgtcggt catgatgtcc ttccggtggg ggttggttct tcaggaatgg
     2461 aagtctgcag cgccgcgcac gaggcgcaca ggccccagta cgtgacctcg gcgacggcga
     2521 tgcggtagcc gtgtgcgtcg tcggggatca ggcagggcgc gtggccggtc gcgcagtcga
     2581 cgtcggtgac cgcaccacag gaggtgcaga tcaggtggtg gtggttgtcg tcgacgcgga
     2641 gctcgaacat catcggatgc cccgcgggtt cgatccggcg cacgagtcct gccgcggcga
     2701 agtcgcccag cgcgttgtag acggactgca ggctggtcga ggcgaccacg tcccggacgg
     2761 tgtcgtgcac ggcgtcggct gtggcgtgcg gatgcgcgag cagcgcgtcg tacacggccc
     2821 ggcgcgagtc ggtgacgcgc aggcccgcag cgcggatcgc cgcctccgcg gcatccgtca
     2881 tcagctcctc gcgcatgcct ccatcctatc ttcttttgag tgaatcaaga aaagagcgga
     2941 tgccccggcg cggcgcgcct cacagcggat gcacagcagc ggcgacatca tgtcctcgca
     3001 cggcgatctc gccgtgcaca cgaccggaag gacatggaag atgtctgagt cagtcagcac
     3061 cacagagaaa ccctccctcg gcgcgaagct cgccgctgag ggattcggca cgttcctgct
     3121 cgtgttcggc ggcgtcggca ccgcgctgtt cgcgtcgaac cacttcaccg atcccggcgc
     3181 gaactcggcg gtctacgtcg cggtcgcgct cgcattcggc ctcaccgtgc tcgtcggggt
     3241 gtacgccttc gggccgatct ccggcggtca tttcaatccc gccgtcaccg tcggcgcggc
     3301 tgctgcggga cgcatcccgt ggcgcgacgt cgcgccgtac atcatcgctc aggtcgtcgg
     3361 cggcgtgatc gcctcgaccg cgctggtcct gatcggcctc ttcggtcccg acaagtggct
     3421 gcagaccgct caggacgcag ggttcgccag caacggctgg gactcgcact cccccggcgg
     3481 cttcggcatg atgtcggcga tcatcatcga ggtcatcctc accggcatct tcgtgatggt
     3541 catcctcggc tccacccacg cgacgcgcgg cacggcgaac gccggcatcg cgatcggcct
     3601 caccctcacg ctgatccacc tgatctcgat cccggtggac aacacgtcgg tcaacccggc
     3661 ccgttcgatc gccgcggcga tctacggcgg cggcgagtcc ctcgcgcagc tgtgggtgtt
     3721 cctggtgttc ccgatcgtgg gcgcactgat cgtgggctac gcctacaagg cgctgttcga
     3781 cggcaccacc agggcctgac gacacgcgga ggcgttcggt ctcacagtat gcaggagggg
     3841 acgactcagg tcgtcccctc ctcgctttcc ggggccgcgc ccctcagcct gcgaacagcg
     3901 tgggcgtgcc ggactcgtac tcgatcaggt cgccggtctc gccgtcacgc gcggcgcgac
     3961 ggcctgtgac cagcatgtag aacagcacga tgccgagagc gacggcgccg attccgatct
     4021 tgacaggcca gggcagcgcc gatccggtga cgaacccctc gacgagcccg gacagcagca
     4081 gcacgaagac caggcccatc gcgacggtgg ccagggagcg cccgccggtc gccagggact
     4141 ccccccgcgt gcgccggccc ggcaccgccc acgaccagaa gatgtgcagc cctgcggccg
     4201 cggcgatgaa gatcgcggtc agctcgagca tgccgtgcgg caggatgtgc agcatcatga
     4261 cgtccaggcg gtcgtgcgag gccatcaccg ccgccgccac gcccagcccc atcgcgttct
     4321 gcacgatcac ccacaccggc cacacgccgg tcacgccgaa gagcacgcac tgcagggcga
     4381 tccaggcgtt gttcgaccac acctgcccgg cgaacgacgc catcggctcg tagtagtcca
     4441 cgaagtcgtg ctccgcgtac tgcttgagca cctccgggct ccccagggac gcgatgcggg
     4501 caggatccga ggagatccag gcgccgctga tcagcacgat cagcacgaag accaccgcga
     4561 cgcccgcggt cgtccaacgc agccgataca gcgccgccgg cagctgcagc cggaagaacc
     4621 gggaggtctg cgcgaggatg ttgtcggatg cgccggtgaa gcgcagacgt gcggcggcga
     4681 ggatcgtgga caggtaggcg ccctgcggcg agtccccgac ggaggtcttc agctcggcga
     4741 gatccgcgga tgccgcacgg tagcgcgtga tgagctcgtc gacctcggtc ccggtcagcc
     4801 gccgcttgcg gctgagggcg tccagccgct cccactcgga gcggcgcatg tcggtgagag
     4861 catcggcatc cacgtgattg actctactca tgtcgcttcc gatcaccgat gagcaggaga
     4921 tcctctccgg cgaagccgtc gccatcgacg tgcagccgat cggcttcgtg ctgcgggccg
     4981 cgggcgccct gatcgacatg gtcatcgggt tcgcggtgtt cgcggcgttc gagatcctgc
     5041 agttctggct gctcagcctg aacctgctgg atctgcacac gctgccgatc ctcagcatct
     5101 gggccgcggt gatcagcttc ctggtgctgc cgatcaccgt cgagatggcg atgcggggcc
     5161 gcagcgtggg caagctcgcc gtgggcgggc ggatcgtgcg cgtcgacggc ggcgcgatca
     5221 gcttccgcca caccttcatc cgcgcactgg tgggcgtgct ggagatctac atgacactgg
     5281 gaggtggcgc ggtgatcgcg ggcgcggtga ccgcgcgctc gcagcggctg ggcgacctgg
     5341 tcgccggcac ctactcgcag cgggtgcgca c
//
LOCUS       DATMSX010000296        32220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_80197,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000296 DATMSX010000000
VERSION     DATMSX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 32220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32220
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80197"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1045)
                     /locus_tag="VN041_08905"
     CDS             complement(143..1045)
                     /locus_tag="VN041_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29197.1"
                     /translation="MTSLRDTLVSGIRRIDAVSPALTGRVANVAFFATTRQMRVRDSD
                     RVTHDAARRESIRIGGHDVVTYQWGPGARAALLMHGWDGRASQFATMVRDLVAEGWRV
                     VAFDAPAHGDSSGRRTDARDWVAAAQRLAASEGPFELVIGHSFGGFASLAAVREGVQA
                     RRVITISAAGSVQAFHDEFERMLRLSPRASEAFERVFHRRLGITRREGVARFDSVTHP
                     LPQEVELLIVHDEYDPRVTIENAHALYRAHGERARLLVTQGLGHNRILGADRVLDAVI
                     AFAAEGPHGIDRADASEQGGVRGA"
     gene            1113..1778
                     /locus_tag="VN041_08910"
     CDS             1113..1778
                     /locus_tag="VN041_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29198.1"
                     /translation="MSDLVTEQQVADGRRLRGDASRHTVLAHATDLASVEGLDGLSIG
                     RLAEASGRSKSSIAGLFQNKEGLQLATVDAGRAVFVSVVVEPARSRPRGLPRLVALMS
                     NVIEYSRSRVFAGGCFFAAISADYDSKPGPVRDAVRSAIEQWYGYLEIQVRTAAGDGS
                     LDLDDEGIEQLAFALPALYEQANARSLLYGSDRPYALAARGMRDLLLQAGADASTLSA
                     LPV"
     gene            complement(1798..3225)
                     /locus_tag="VN041_08915"
     CDS             complement(1798..3225)
                     /locus_tag="VN041_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29199.1"
                     /translation="MSDDHSAAGTPINTAAMTAFGREHDDVAPASRKKVISWAMWDWA
                     LQPFNTVILTFVWIALYLTSRMFLDPDVRASGLLADGSYMNCNKSEYLGSTYCSSLTD
                     LSEWWGWANFAAGIVILVLAPVLGQQADARGNKKKWVIGATIGVALLQFALFFVEADP
                     KFFWFGAFAVAIAAVISEIGNVSYYAMLSEVSTPKNVGRISGLGWGLGYIGGVVALIV
                     CVPVLFLIGQDQPLAFKLVAVICAVWTIVFSIPLFRNVPESPAYASSERVGFFRSYVV
                     LAKNIGKLFRDNRPTFWFMLSAAVYRDGLAGVFAFGAVLAAQGFGFSFLEVIVFGLAA
                     NLVAGVSTLLAGRIDDAIGPRRLIVIALSGLVVMAFIVFALKDYGTIVFWICGLLLCA
                     FVGPTQAASRSLLTRLAPPSQQGEIFGLYATTGRVASFLSPLAWSLFLAWFGGIVYGV
                     LGIGLILLIGLVLLLFVRFPKDVGV"
     gene            complement(3311..4162)
                     /locus_tag="VN041_08920"
     CDS             complement(3311..4162)
                     /locus_tag="VN041_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HWU29200.1"
                     /translation="MNRQIPASFDASPFDVTLAFADTVLQENQRWSTWPATMPTERGP
                     LPRPDWVVTSAAAVDTELGILKTGKEADVFLIERAVPDEPGQRTLLAAKRYRDSDHRS
                     FQRSSVYTEGRRTQNTRDARALAKKSAHGREVAAAQWSFAEFAALSRMHELGAPVPYP
                     VQVDGTEILMEFLGDGHAAAPRLAQVRSDAVDLADLFDQIVDLMRIFAAAGFAHGDLS
                     AYNLLVHEGRVRVIDLPQIVDTVANPQGLDLLHRDCVNICDWFTRRRLERDPEELFAE
                     LLAASFG"
     gene            4350..4697
                     /locus_tag="VN041_08925"
     CDS             4350..4697
                     /locus_tag="VN041_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29201.1"
                     /translation="MLICYPFVMVIVGMDRPWPTWRIGCIFTASKRREQGVARVAVAA
                     ALEEIRKAGGGVVEAYPEQLTDRKPQRGAYLHTGPEELYTRFGFVRDRRIAKWRWVMR
                     AEISAEERPAEHL"
     gene            4828..5289
                     /locus_tag="VN041_08930"
     CDS             4828..5289
                     /locus_tag="VN041_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HWU29202.1"
                     /translation="MFRSPARLFRALAIAEAITWTLLIGALIARAVGAAPVLVTIAGG
                     IHGFVFLSYGATAVLLAFHQRWHVGVAIAAIASAIVPYATIPAEIWLHRSRRLEGDWR
                     LTETDDPRDRTWYDRTMRWFLHRPWVLALLILVAVAAIFSILLLIGPPGGK"
     gene            complement(5290..6669)
                     /locus_tag="VN041_08935"
     CDS             complement(5290..6669)
                     /locus_tag="VN041_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HWU29203.1"
                     /translation="MSATASSSIRPEDAKLGWRANIAYGFQHVLTMYGGIIAPPLVMG
                     QAAGMSQADLGMLVACSLFMGGLATLLQTLGIPFFGSRLPLVQGVSFSGIATMLAIQG
                     DGGIQAVMGAVIVSSLIGILLVPLFAMVLRFFPPVVTGTVITSIGLSLMPVAAGWAMG
                     PDAKDPAFGSPGNVGLAAATVVIIIVLTKLGTPSIARLSVLLSLGIGTILAVFLGAAD
                     FSGVADGPVFALPTPFAFGLPTFDVASILSMMIVILVTFTETTADILAVSEVVGTKTD
                     AKRIADGLRADMVASAVSPVFNSFTQSAFAQNVGLVAITGVKSRFVVATGGVILIGLG
                     VLPVLGRVIAAVPTPVLGGVGIFLFGSVAASGIATLAKVDYRNNLNLMIVAGSLGVGL
                     LPLMHPGFYAGFPDWFSVIFNSAISSTTLTAVVLNIVFNHWVRRSKNDGRSEFAAAAE
                     HPKAEKSED"
     gene            6785..7399
                     /locus_tag="VN041_08940"
     CDS             6785..7399
                     /locus_tag="VN041_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HWU29204.1"
                     /translation="MPVLEREHALSAVVLVEGRSDRLAVEALARRRAKSPAGAGPLDG
                     VELRELDGITNLRKALTALRGTSARVLGLYDAAERRYVQRVLAGLGMLDDAGRGAQGT
                     PDMDPLEPLGFFGCERDLEDEVIRAAGAELVLEALDPRERARFRRFQRQPAQRVRSIE
                     EQLHRFAGTAAGRKSRFAADVIDAVPLERTPATLNRMLDRVGDR"
     gene            7560..9422
                     /gene="dnaK"
                     /locus_tag="VN041_08945"
     CDS             7560..9422
                     /gene="dnaK"
                     /locus_tag="VN041_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWU29205.1"
                     /translation="MARAVGIDLGTTNSVVSVLEGGEPKVIANAEGFRTTPSVVAFTK
                     DGEVLVGETAKRQAVTNVDRTISSVKRHMGTDWSFDVDGKKWTPQEISARILMKLKRD
                     AEAYLGDTVTDAVITVPAYFNDAERQATKEAGEISGLNVLRIINEPTAAALAYGLDKG
                     KEDELILVFDLGGGTFDVSLLEVGKDDDFSTIQVRATAGDNRLGGDDWDQRVVDYLIK
                     QFKDSTGVDVSGDKIALQRLKEAAEQAKKELSSSTSTSVNLPYLSLTESGPVSLSETL
                     TRAKFEDLTKDLLDRTKKPFEDVIREANIKVSDIDHIVLVGGSTRMPAVAELVKRETG
                     KEANKGVNPDEVVAVGAALQAGVLKGERKDVLLIDVTPLSLGIETKGGRMTKLIDRNT
                     AIPTKRSETFTTADDNQPSVAIQVFQGEREFTRDNKPLGTFELTGIAPAPRGIPQIEV
                     TFDIDANGIVHVSAKDKGTGKEQSMTITGGSSLSKDDIERMVREAEEHAAEDKKRAEA
                     LEQRNQAESLAYSIEKLIKENEDKLPEDVKSEVQGDVDALKTALAGEDDDAVKAAFDK
                     LNASQGKLGEAIYAQSQADAAPGAAAGDAGASADAGSDEDVVDAEVVDDEDESK"
     gene            9425..10060
                     /gene="grpE"
                     /locus_tag="VN041_08950"
     CDS             9425..10060
                     /gene="grpE"
                     /locus_tag="VN041_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWU29206.1"
                     /translation="MTDKNFDENDEVQPEGAGSDAAASGPEPQVNQDSAEASAAEGSD
                     DDFTVDDILNAAQTPEASSEDGSDAGIVDAEHALLNDLKRLTAEYANYRRRTEEQRHV
                     EIARAKGEVAKGLLPVLDDLDRAQQHGDLEEGSPFAVISEKLRGVVEKLGVVAYGEKG
                     DEFDPQQHEAIFQQPTPGATTSTILEVVEVGYRLGDVELRPAKVVVAVPAE"
     gene            10068..11051
                     /locus_tag="VN041_08955"
     CDS             10068..11051
                     /locus_tag="VN041_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWU29207.1"
                     /translation="MASQDWFDKDFYKTLGVSKDISDAELKKTYRKLARKYHPDSNPG
                     DAAAEAKFKEISEAYSVLSDAEQRREYDEIRAMGSGTRFTAPGGGGAGGFEDVFSRFG
                     QGGGRQADFDDIFSMFNQGGGSFGGGRFGQSSGGFRGFGGPQKGADVTARTTLDFVTA
                     AKGETITLQAEDGKPFKIKVPAGVADGQKIRLRGRGRPSPDGGEPGDIVVQVAVRPHP
                     VFTREGLNLRLTVPVTFTEATLGATIEVPTLGGETVKLRVAPGTPSGRVLRVKGRGIA
                     SAKKGTGDLLAELQVAVPSHLDDAAKEALQRFHELEPTENPRAEMMAKAKA"
     gene            11053..11523
                     /locus_tag="VN041_08960"
     CDS             11053..11523
                     /locus_tag="VN041_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU29208.1"
                     /translation="MDAESPVFAIAVAAELAGMHPQTLRQYDRIGLVVPGRTRGGSRR
                     YSMRDIEQLREVAQLSSEGMSLPAIARLLDLEDEVRMLRSRVTELEGALRAERESRPG
                     VRVFAAGATGSIVSLASGRRIRRSTEVVLWHPRTVSTSEHSDGTASDPPLPGTD"
     gene            complement(11533..11949)
                     /locus_tag="VN041_08965"
     CDS             complement(11533..11949)
                     /locus_tag="VN041_08965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU29209.1"
                     /translation="MDAETRSIQVEGAADLALVERVLDALDGLWITAPEVPGEDQSLF
                     RLAIAEISTNVVTHSEGHVTMSVSARVTPGTLHALIQDTAAPVTIELAAAAMPDAEAE
                     SGRGLALARASVDVLEHHPAPSGNVWVLRRSFTPQQ"
     gene            complement(11939..12271)
                     /locus_tag="VN041_08970"
     CDS             complement(11939..12271)
                     /locus_tag="VN041_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWU29210.1"
                     /translation="MNLTTAIRDHYAVIDIVGRLTATGAPQLRNAVNELIDAGSNRIV
                     IDMSRTEFVDSTGLGALVGGLKSARVAGGDLRVAAVQDSVRTVLRLTNLDRVLRNHET
                     PESAFDGR"
     gene            complement(12268..14217)
                     /locus_tag="VN041_08975"
     CDS             complement(12268..14217)
                     /locus_tag="VN041_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU29211.1"
                     /translation="MKARFALIRVLAVLSALLGVNYVAWRWLESVNWSAWWIAVPLVI
                     AETYSLIDTLLFCLTMWRARERSGPSSPPRGTADVFITTYNEPIEMVMTTARAAKAIA
                     YPHSTWILDDGSRQDLEQAARDAGIGYLTRSEDWTGKPRHAKAGNLNSALFQTDGEFM
                     LILDADQIPDPLILHRTLGYFADDPEVALVQTPQWFSNVDDADPLGSQAPLFYGPIQQ
                     GKDGWNAAFFCGSNAVLRRESLMQLGIVGYVRDVTTSTARALRVAAALAGREARRQKD
                     PALAAELTQLRSVIEKARSAVNAGEPVAEATFDVRAAVERASQALVARDFATIRADLD
                     VIARLPVARDAELDAVVVDDKALDGLAMADLSPLGAIDAVRALLDALRIDRGDEAQPI
                     LPLATISVTEDMATAMQLHALGWRSVYHHEILARGLAPEDLRTMLTQRLRWAQGTLQV
                     MLRDNPLLKRGLSIGQRLMYFGTMWSYLSGFAAIVYLAAPVIYLLFGVLPVKAWSVDF
                     FARFLPAFAANQLLFIVIARGMRTWRGQQYSLALFPVWIRACWTAFANVVLGRPLGFA
                     VTKKDGRDDRGVPWGAIWPQFTAMVVLAVALVIGVIRVIVGTADGTGTLVNAIWVAYD
                     LVVLSVIIGAARYRGPVARPSEGDV"
     gene            complement(14214..15368)
                     /locus_tag="VN041_08980"
     CDS             complement(14214..15368)
                     /locus_tag="VN041_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HWU29212.1"
                     /translation="MFDERRRQLALEELGVLDTQPDERVDRVARLAREMFGVPMVSVS
                     LLDHDRQWRKSQIGLGGDEAPREDSFCDYTVRQNRTVVIEDARATAEFAENPFVVGDP
                     HLRFYAGHPLHAPGGEPVGTLCVLDTVPHTLTVAQRDLLRDLAFWVQTELAKDQEVDH
                     AEVLQRSMQPSVLPSPDGYEIAAGSVSRGRLAGDFYHLELQHGALRITLADAMGKGIG
                     PALVAAGVRASLRTDPRRALTAAIDEADRLLEEDLGDRTMFVTAVHAELKLDTGSLEY
                     VDAGHGLAFVLRADGSWQSLRSTGLPLGMGMAFTEPRRSDFVEVRPGDHFICCSDGLL
                     DVLDADDPFGHVERVLRTAGPKGAVQEALRLAGGAQATDDVTVVVVSRTR"
     gene            complement(15450..16805)
                     /gene="mgtE"
                     /locus_tag="VN041_08985"
     CDS             complement(15450..16805)
                     /gene="mgtE"
                     /locus_tag="VN041_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWU29213.1"
                     /translation="MTPTQLQTVSEAADEIRRLIAQGDLPGASAVLTPLAVPEVVELV
                     DRLPIDDAAIVYRLLAKSRALEVFEALSPGTRGDLVQALRNVEITALFAEMDPDDRVW
                     LLDELPASVAPQLLRGLTRQERDLTSGMLGYPQDSIGRRMSPEFVTTHPQLTVRETFA
                     RIRAALDSAETVYTVPVTDARRVVGVVSLRDLMSADEDAAISDVMSEPHVAEVTENAE
                     TAARRATDYSLLALPIVDGEQRLVGILTIDDAARILKQEESEDAARQGGVEPLRRSYL
                     GTPIRQLVRSRVVWLLVLAVGATLTVQVLDTFEATLAQMTVLALFVPLLIGTGGNTGN
                     QAATTVTRSLALGEVSPRDVLRVLSREIRVGFCLGLLLGTLGFVIAGLIYEWHVGLVI
                     GLTLVAVCTVAASVGGMMPLAARAIRVDPAVFSNPFITTFVDATGLVIYFLIAKTVLG
                     L"
     gene            17052..17717
                     /locus_tag="VN041_08990"
     CDS             17052..17717
                     /locus_tag="VN041_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29214.1"
                     /translation="MSDTVRRGESLGAQIANVLRQRIVRGVLAPGERITEEALAEEFD
                     VSRGPVRDALTQLSFEKLVRIQRPRGVFVVGLTGEDVDELYSLRGALEQLALSRAIRV
                     SDDARWRTMADAVQRMADAADAGDHAAFVEADLDFHSQIYVLADHARLEGAWKQYLPT
                     FTALLDVTINHDDDLHDSAGDHVVLMDVMRSGDAARAAEVLTAHLDGARDRMLSELPQ
                     QTP"
     gene            17768..18793
                     /locus_tag="VN041_08995"
     CDS             17768..18793
                     /locus_tag="VN041_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWU29215.1"
                     /translation="MNTATLASVDNTVDQHRGAKMPRIRMSRAAAAIVAVTATALVLA
                     GCTKVEEGGAESAFPEKDIRLIIQANPGGGSDLSSRALATELEKILDVSVVPENMPGA
                     AGALAMEYVGSQKADGYVIGFAPVEIAMLNTTQGAKVLPENYDLLGQVMLAPGVITVG
                     ANSGIDSLEALVAKAKSGAVTVANSGAGSIWEAATVGLADATGAQFSPVPYDGGATAV
                     GAAASGETVAAVSGLGEALAQGDAVKILAVMHDERHPDAKDVPTVEEAIGEKVEFGGW
                     GGIYTAKGLPDDVHKTLEDAVKKAVESDGYQKFQTEAGNLVVYRDSAEWTSFVKDQFT
                     LFKDLLG"
     gene            18847..19374
                     /locus_tag="VN041_09000"
     CDS             18847..19374
                     /locus_tag="VN041_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HWU29216.1"
                     /translation="MSTTDTPVEDEYQSAAPSRGLEIAFAVVALAFAAGYLFLATQIP
                     LRREAAAGQMDARFWPTVIGVAAVALAVILLVIAISRPAPSREDVERIQPGGVLRMAA
                     TLAIACVFVALWSLGSVVLMGYRIEIFPIAAGLLMASLMLVYGHRRWLSLIIYTVAVT
                     AFVYVVFGMLLRIPL"
     gene            19371..21020
                     /locus_tag="VN041_09005"
     CDS             19371..21020
                     /locus_tag="VN041_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWU29217.1"
                     /translation="MNAFAEGLNALLDVQILLNMGVGLVLGFMVGAFPGITATMAVAL
                     AAGFTMTLEPVQGLAVMLTIYVAANFGDRVPSILINTPGTPASIATTLDGYPMAKQGK
                     AGLALTVSAIVSAVGILASLVLFSVAAVPIASFARDYFRSPELFALVIFGIAIMIGIS
                     SKSMVKGILAGLFGLMLGTVGTYAATADQRFTFGSLELVEGVNFIAVIIGLFGIAELF
                     DQLLTHRKNRVRPISSLGRWWPTWGEMKQTGRATAISGGVGLGVGLIPAAGGDIAGLI
                     GWEQARRASKHPERFGKGSIEGVAASDTASSATLGGSLTTTMALGIPGDSVMAVMIGS
                     MIIWGITPGPTLFTNRPDLVVSIVGIMLLATLLSLGLSLVRMKGMVKLLDVPQQYLWS
                     GILIFCIIGTYATSNSLSTVITMLVFGVVGVLLKRMQVPAGPVVLGLLLGPLAEENLA
                     RTMAILPTKPFFSVVSPIAIALLVLAVLSIALPAIRAARKPREQRASLEESILPTSSI
                     EQIEHAREELAQDPELLTSTVRNIEKKGARRARRTDKENEK"
     gene            21017..21886
                     /locus_tag="VN041_09010"
     CDS             21017..21886
                     /locus_tag="VN041_09010"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWU29218.1"
                     /translation="MTRYDILTAIPTAFGRDGSLDLEGSRAIFRYVGKSGNEGAFVLG
                     TTGEFPAVDLDEFTALVSAALDELQDRMRVVVHVGRPSTFEAVRLTRIARELGATEFA
                     ALTPYYLRASDDAVFDYFRQVSDAVGDGRLYVYIYPARSGNPVSPELLVRLAGLPNVV
                     GAKVSELSLDELAAYRAAVPADFELYTGADRDLIAAVDAGAQGVVSGVSSVTPQPFRA
                     LADAGRSGDASAIAAAQAAVDEVVALIGGDMARMKEGYRVLGVADSYCRMALDEPTDA
                     EREAVARVVAAYA"
     gene            complement(21974..22942)
                     /locus_tag="VN041_09015"
     CDS             complement(21974..22942)
                     /locus_tag="VN041_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29219.1"
                     /translation="MRLTERFTEAEPATADGIWLRPTGVADAEPRWGHAHGMQIGIHP
                     IPGPRGLLRVFTPYLDHPRERLVNFIAVEPIPRGEDRRGYSELEPSSLDPDENGKRFW
                     SGDDPDGLAAPRDPLAPSRGVVETIDGVETLTVWIGVERFDNGADVRVRVRFRADRPR
                     EVEVAGFENEGSAPLQRLVLTATMGNWARLRRLELADRTVTPAELWPGFGGTGFTEHG
                     RFGLAELRREGQTAAVSAIGDEEEPWSVAYADDTAEHWRFVGLRSRQTWRVDDPHPDL
                     EVLVNARWSYWASASEIPGGPAFENFEISEPFRQGASFRFAVDPLD"
     gene            23019..24170
                     /locus_tag="VN041_09020"
     CDS             23019..24170
                     /locus_tag="VN041_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HWU29220.1"
                     /translation="MSHLTSAQRALATVVVAASALALGACSASGPIAVDPDPDKPFVL
                     AGVSDVTEIAQLTGPGAMNDTASVGVAGTDLGSMVNVGERTFFLFGDTFGERDPDSVG
                     GQGGFWRSNVAAWTTDDDPTDGITFDGWHVDDVGLATALVEGDHDSNDIGGEVTKIPT
                     YGFTVGDDMYVSYMSVSHWGDPGAWDANYSALIASSDGGDSWHPVPGVRWPGDSNFVQ
                     FATAHVTDGGTEYVYIWGIRSGRFGGVQLMRVRSERAAVESQSGYEYFAGADGSQPHW
                     SADMTDAATVLEGTIGELSVMWSAYLDRWIMTYSDAGNAYIREGATPWGPWGEPIELA
                     SASDYPGLYSPYLNPRYTSADGRIVYFTMSLWGPYNVFWFSARLDRRVP"
     gene            24313..25686
                     /locus_tag="VN041_09025"
     CDS             24313..25686
                     /locus_tag="VN041_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU29221.1"
                     /translation="MAINTSRKRRMILSALGAVTAVGLLAGCGSSTPGQGAGEGDGGS
                     GGATTVQFWHRTFTPVENAWYKDIVKKFNASQDKIKVVDTEVPADAWDQKMKAAQAAG
                     KAPDIYTHSGSIQDAVNAGQLYELNKVVDQSKLDEIIDAAKPVSEIGGKYYAYPLLLE
                     AQTVLFWNKEMLTKAGVDAEAGPTTWDELLADCAKIQPTLAKGTYCISPASDAVTFAW
                     STVGQQYNFSGHTALTDDWTKPDINNDGYKSLMLKYKELWDKGYMAKQALDAYVGGKD
                     FGEKKVAFKVSGSWMMSQIGSDYKDMLTNTGVGAFPNASGADGRTATTLGNFKWVIDA
                     KSKNPEAAGKFLEWALAGDTANLVPFFVDTQFTKVPVRKSVQDAVAKDEAAAKAPWSK
                     VVVDDIAPTAIPEPTYPWDVSLAVGTAMESVMKGAATPDAAIATAEKAIQTVIDREKL
                     PEKAPKN"
     gene            25756..26682
                     /locus_tag="VN041_09030"
     CDS             25756..26682
                     /locus_tag="VN041_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU29222.1"
                     /translation="MTTAERGVRRQQRQRHDRYRYRDTKLALLMLAPVLILLGIFVIW
                     PAFYAGFLSFQDWSFYKEPVFVGFKNFTDVLSDPLFIATIGRGFVFVLMTVPPMLVLA
                     YLFSSLVVAVSRRFASILKVSIYVPTIISGVITSIIFVLIYDYSGGLLNAFLGAFGID
                     PIAWIGDPAWALLAIAIPAVWLGMGLTSLIMVAAMVDIPTEYYEAAAMEGAGWWQKTF
                     YITIPQMKNVFLYLLVTGFVAAIQQFELPLVMTGGGPLDSTTLPNLFIFNHFRNDINV
                     GYSIAAALLLFVVLGSVSALVFKFVNSERLVD"
     gene            26682..27581
                     /locus_tag="VN041_09035"
     CDS             26682..27581
                     /locus_tag="VN041_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU29223.1"
                     /translation="MAVMQGETTRIVLAGKEPSGRSKLPRTSGGWLALAGKVVVGLIF
                     LVAALFPLAWMVIAGFKSKTEVVSTPFQFFPEVWRWQNYAQILADPTFTRTMLWTFLG
                     AVLFTVLSLTVNSLAAYAFARLNFRFQRTLWVAVITTMFIPGMTILLTSFIVVTRLSM
                     LDTLAVLVIPGAASAGAVFFIRQFYLNIPASLEEAAMLDGCGRFQIFWRIFLPLSKPP
                     FVVMGITAFLAYWNSYVWPIMTITTPERFVVQQYLATFRSERSTELGLLMAGSVLAAA
                     PVVILFLIFQRQIIGNIKMAGLK"
     gene            27595..28206
                     /locus_tag="VN041_09040"
     CDS             27595..28206
                     /locus_tag="VN041_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29224.1"
                     /translation="MLLSVTGASGTGKSTALDLLREVDFGTPVICVEFDSIGVPDGAD
                     TAWRHDATERWVRFALEAQSRGEHVLLFGQVPPGELLAVPSAEQLDGLAICVLAASPH
                     AQEARLIGRGEPADSLVHHLRFGQWFRSHSEDPQHAPEVIRVDTPVPMEWSRWETLSG
                     DDPRWPVTVLDTDALSAGEVAERIEAWTRKNLADVESAEPRGR"
     gene            28235..29344
                     /locus_tag="VN041_09045"
     CDS             28235..29344
                     /locus_tag="VN041_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HWU29225.1"
                     /translation="MTEQYRQPWMSRRALLQGALAAGAVGAASAVGIAAAPAATAAGP
                     LTVTRMKVLAGTQTSVQYGVGATDLGIPARTPDGRLLFVFGDTWADHVGGANWRSPVA
                     LYSSTTNLTAGVTFNGCAGAGTATTGVAPQLWYYPHDAYFKTVIPSDVITIGSRMYLH
                     AIVNGPEFGMVRWTELWASDDSGGTWFHTGLKFPADMAGGRFQCITWGLGNDGYVYIY
                     GTGFQRDKGIVLYRVASSQMTQLSAYEPWGFANGSWGWGKPATEVLPGKFGEMCLRPL
                     GGKWILTWFNAADYRIDAMVLNTPTDNLYTAAKTTLLWGGSWGSEDATHVAQLYGGYI
                     IPGSTLGDLHLSVSQWNTGNSSVYHSEQFRVQGLV"
     gene            29367..31094
                     /locus_tag="VN041_09050"
     CDS             29367..31094
                     /locus_tag="VN041_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29226.1"
                     /translation="MIVLPEKYTPPPAGVTPAGFLVWLVRMQWTTVLQGVVCDVIWLL
                     GLALTPWAIGRAVDEGLVAGDYGAFLRWLGVVVWLQLQHSLIQGLRDRAGSINYERGV
                     TRVDQVIVHTATRITTAADRRLGAGSVVTMASNDAWWFGFVPINVGTLVSALASFITV
                     SVLLLRDSVLLGVLVIVAVPSFSMLQVSLVNALRSRQSAAREATREMNSVAADSVRGL
                     RVIRGIGGEDWFLERFRDRSDRARDAQKTVAGPVSLADALSVFIAGVLVVGLTWIGAA
                     MVARGELRVGELVAFYGYAGFMVLPVTLFNQVIRVVVRGMIASRAIVEMLDVESPWPD
                     AADRPAPDRPSQRPGALHDKRTGLTVRDGEQLAVVVRDSQEAQALVDRIARLAPDGED
                     AVVLDGRLQSSIPVQSVRRRIVASDPVPFLFSGTLRELLDPWGRHDDGEILRAVSAVD
                     AADILAPLDEGLDVRVGERGIEFSGGQRQRLGAARALLADAPVLLLHDPTSAVDAATE
                     ERMATGIRSHRAGRTTVIVTTSPLVLSSADRVVVIEESRIRIEGTHEELVRGDADYRR
                     AVLRAEGVS"
     gene            31091..>32220
                     /locus_tag="VN041_09055"
     CDS             31091..>32220
                     /locus_tag="VN041_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29227.1"
                     /translation="MTGHALPRADARRVRAELRAIWQQHRARFLLVILLNGAAAASGL
                     LPPRLVGLMIDRLRDGADAGEVALYCGAMLAGVIAQSLFVLTAARTAWKLGEDVFAGL
                     RLAFLSDALAVPMGVVESAGTGELVTRTTQDINAVSETVRWVLPGVLVSALTVVLTLI
                     AAVVASPLITPVFLLAVPLLVLMMRWYVKRAPRVYQRMGDSYGPVFATMDETANGARA
                     VEALSLQEARQRSLASALAGHWTAAVGRIRLRQTMLPWSNLAFAIPVFCSLAWGGWLA
                     VHGEVSVGTVVTVTLYASALVAPLESLIDWTDELQRSFVSFARILGVGEIVDRSPHAG
                     QQPASTDVALHGVGFAYTEGREVVHDVSLRVVPGERLAVVGPS"
BASE COUNT         5173 a        11017 c        11037 g         4993 t
ORIGIN      
        1 gacgagggca cgcgcatgtt ccggggcatg cgtggcgaac ggcgcgaagc gctcggccgc
       61 gcgctcgccg ccttcaccga ggaggagagc gttgaactcg ctcggctgct gacgaagctc
      121 gccgacaact ggggggcctg actcaggcgc cgcgcacccc gccctgctcg ctcgcatccg
      181 ctcgatcgat gccgtgcggt ccctccgcgg cgaacgcgat caccgcgtcg agcacgcgat
      241 cggccccgag gatgcggttg tggccgagtc cctgagtcac cagaaggcga gcccgttcgc
      301 cgtgcgcccg atacagggcg tgcgcgttct cgatcgtgac tcgagggtcg tactcgtcgt
      361 gcacgatgag cagctccacc tcctgcggca gggggtgtgt cacggagtcg aagcgggcga
      421 cgccctccct gcgtgtgatg ccgagtcggc ggtggaagac gcgctcgaac gcctcgctcg
      481 cgcggggcga cagccgcagc atccgttcga actcgtcgtg gaacgcctgg acggatcccg
      541 ccgccgagat ggtgatcacc cggcgtgcct gtacgccctc tcggaccgcc gcgagggacg
      601 cgaatccgcc gaaggagtgg ccgatcacga gttcgaacgg cccctcggat gcggcgaggc
      661 gctgggctgc ggcgacccag tccctggcgt cggtgcgcct gcccgatgag tcgccgtggg
      721 cgggggcatc gaaggccacg acgcgccacc cctcggcgac cagatcgcgc accatcgtcg
      781 cgaactgcga ggcgcgcccg tcccagccgt gcatgagaag tgcggcgcgc gcgcctggtc
      841 cccactggta ggtcacgacg tcgtgtccgc cgatccggat gctctcgcgg cgcgccgcgt
      901 cgtgggtgac gcgatcgctg tcacgcacgc gcatctgccg ggtcgtggcg aagaatgcga
      961 cgttcgcgac gcggccggtg agggcgggcg acacggcgtc gatccggcgg atgccgctca
     1021 cgagggtgtc tctcaacgaa gtcatgccga actcctgact atacgaacga tcgtacatag
     1081 agtatccgaa cgatcgttcg tagactagga gcatgtccga tctggtcacc gagcagcagg
     1141 tcgcggatgg gcgtcgcctg cgcggcgacg cctctcgcca cacggtgctc gcgcatgcga
     1201 ccgacctcgc ctcggtggag gggctggacg ggctgagcat cgggcgcctc gccgaggcat
     1261 ccggacgcag caagagcagt atcgccgggc tcttccagaa caaggagggg ctgcagctcg
     1321 ccaccgtcga cgccggccgt gccgtgttcg tctcggtggt cgtcgagccc gcgcgcagtc
     1381 ggccacgcgg acttccgcgt ctggtggctt tgatgagcaa cgtgatcgag tactcccgca
     1441 gccgcgtctt cgccggaggc tgcttcttcg ctgcgatctc cgccgactac gactccaagc
     1501 ccgggccggt gcgcgatgcc gtccgctcgg cgatcgagca gtggtacggg tacctcgaga
     1561 tccaggtgcg cacggccgcc ggcgacggat cgctggacct ggatgacgag ggcatcgagc
     1621 agctggcatt cgcgctgccg gccctgtatg agcaggccaa cgcccgttcg ctgctctacg
     1681 gcagcgaccg tccctacgcg ctcgcggccc ggggcatgcg cgacctcctg ctccaggccg
     1741 gagcggatgc gtcgaccctg agcgcgctgc cggtctgacg gaagacgccg cggaggctca
     1801 gaccccgacg tccttcggga agcgcacgaa aagcagcagc accaggccga tcagcaggat
     1861 cagcccgatg cccagcacgc cgtacacgat cccgccgaac caggcgagga acagcgacca
     1921 ggccaggggg gacaggaacg aggcgacgcg accggtcgtg gcgtacaggc cgaagatctc
     1981 gccctgctgg ctcggcggag cgagccgggt gagcaggctg cggctggcag cctgggtggg
     2041 gccgacgaag gcgcacagca gaagaccgca gatccagaag acgatggtcc cgtagtcctt
     2101 gagcgcgaag acgatgaacg ccatgacgac caggccggag agcgcgatca cgatcagccg
     2161 ccgggggccg atcgcgtcgt cgatgcggcc ggcgagcagc gtggagactc cggcaacgag
     2221 gttcgcggcg agtccgaaca cgatcacctc gaggaacgag aagccgaagc cctgcgccgc
     2281 gagcacggcc ccgaaggcga agacgccggc caggccgtca cggtagacgg cggccgagag
     2341 catgaaccag aaggtcggac ggttgtcccg gaacagcttt ccgatgttct tcgccagcac
     2401 gacgtaggag cggaagaagc ccaccctctc ggagctcgcg tacgcggggg actcgggcac
     2461 gttgcggaac agcgggatcg agaacacgat cgtccacacc gcgcagatga cggcgacgag
     2521 cttgaaggcg agtggctggt cctgcccgat gaggaagagc acggggacgc agacgatcag
     2581 cgccaccacc ccgccgatat agcccaggcc ccagcccagg cccgagatgc ggccgacgtt
     2641 cttgggtgtg gagacctctg agagcatcgc gtagtagctc acgttgccga tctcgctgat
     2701 cacggctgcg atcgccacgg cgaaggcgcc gaaccagaag aacttcgggt ccgcctcgac
     2761 gaagaacagc gcgaactgca gcagcgcgac gccgatcgtc gccccgatca cccacttctt
     2821 cttgttgcct cgtgcgtcgg cctgctggcc cagcacgggt gcgagcacca ggatcacgat
     2881 gccggccgcg aagttggccc agccccacca ctccgagagg tcggtcaggc tgctgcagta
     2941 cgtggagccg aggtactccg acttgttgca gttcatgtag ctgccgtcgg cgagcagccc
     3001 cgaggcgcgt acgtccgggt ccaggaacat ccgcgaggtc aggtacagcg cgatccagac
     3061 gaaggtgagg atgacggtgt tgaacggctg cagagcccag tcccacatcg cccacgagat
     3121 gaccttcttg cgggaggcgg gcgccacgtc gtcgtgctcg cgcccgaatg cggtcatcgc
     3181 ggcggtgttg atcggtgtgc ccgccgcgga gtgatcgtcg ctcatgcttc gaagtctggc
     3241 cgccgccggt gaacggccgg tgacggcgcg ccgtgcggga tgccgtggtc ggcatccgca
     3301 ggcgccggcc tcacccgaac gacgccgcga gcagctcggc gaacagctcc tccgggtcgc
     3361 gctcgaggcg gcgccgggtg aaccagtcgc agatgttcac gcagtcgcgg tgcagcaggt
     3421 ccaggccttg cgggttcgcg accgtgtcga cgatctgcgg caggtcgatc actcgcacac
     3481 gcccctcgtg caccagcagg ttgtaggcgg agaggtcacc gtgggcgaag ccggcggcgg
     3541 cgaagatgcg catgaggtcg acgatctgat cgaacaggtc ggcgagatcc acggcatccg
     3601 atcgcacctg cgcgagccgg ggcgccgcgg cgtgaccgtc gccgaggaac tccatcagga
     3661 tctcggtgcc gtccacctgc accggatacg gcaccggcgc gccgagctca tgcatccggc
     3721 tgagcgcggc gaactccgcg aacgaccact gcgcggcagc gacctcgcgg ccgtgcgcgg
     3781 acttcttcgc cagggcgcgg gcgtcgcggg tgttctgagt gcgccgcccc tcggtgtaca
     3841 ccgaagagcg ctggaaactg cggtgatcgc tgtcgcggta gcgcttggcc gcgagcagcg
     3901 tgcgctggcc gggctcgtcg gggacggcgc gctcgatcag gaagacgtcc gcctccttgc
     3961 cggtcttcag gatgccgagt tcggtgtcga ccgcggcggc gctggtcacc acccaatcag
     4021 ggcggggcag cggtccgcgc tcggtgggca tggtcgccgg ccaggtcgac cagcgctggt
     4081 tctcctgcag cacggtgtcg gcgaaggcga gggtgacgtc gaagggagaa gcgtcgaagg
     4141 acgcggggat ctgtctgttc acgggaatgg ctccggacgg gaggccactc gcggttcagg
     4201 agcgggcggc ctcgaggatg aggatgtcga cggcggtggg cgcgacaatg acggtgttca
     4261 tcgtgggctc cttccgctcg catcctccgg gcgcgtcccg ggtgcgaggt cgagtctagg
     4321 ggcgacgtcg tctgcgaggc gacgggcgga tgctgatctg ttacccgttc gtgatggtga
     4381 tcgtgggaat ggacagaccc tggcccacgt ggcgcatcgg ctgcatcttc accgcgtcga
     4441 agcgccgcga gcagggcgtg gcacgggtgg cagtggctgc ggcgctggag gagatccgca
     4501 aggcgggcgg gggagtcgtc gaggcgtacc cggagcagct gacggaccgc aagccgcagc
     4561 gcggggcgta cctgcacacc ggcccggaag agctgtacac ccggttcggc ttcgtccgcg
     4621 atcggcggat cgcgaagtgg cgctgggtga tgcgcgcgga gatcagcgcc gaagagcgtc
     4681 cggccgagca tctctgacgt tcaggacgcg ccgcggtctc cacctttcgg atgatcttcc
     4741 tgtcccagag gcccctcaga gccggaattc caggggacgg aaggcatcgg tcgcctagcg
     4801 tggaagcaga tcgacccgag gagatccatg ttccgcagcc ctgcccgact gttccgcgcg
     4861 ctcgcgatcg ccgaggcgat cacctggacc ctgctgatcg gcgcgctcat cgctcgcgcg
     4921 gtcggcgcgg caccggtgct ggtgacgatc gccggcggca tccacggctt cgtcttcctc
     4981 tcctacggcg cgacggccgt gctcctggcc ttccatcagc gctggcacgt cggcgtcgcg
     5041 atcgccgcga tcgccagcgc gatcgttccc tacgcgacca tccccgccga gatctggctg
     5101 caccgcagcc ggcgcctcga gggcgactgg cgactcaccg agaccgacga cccgcgcgac
     5161 cgcacctggt acgaccggac gatgcgctgg ttcctgcacc gcccgtgggt gctcgcgctg
     5221 ctgatcctcg tcgcagtcgc cgcgatcttc agcatcctgc tgctgatcgg ccctcccggc
     5281 gggaagtgat cagtcctcgg acttctcggc cttcggatgc tcggcggctg ccgcgaactc
     5341 gctgcggccg tcgttcttcg accgtcgcac ccagtggttg aagacgatgt tcagcaccac
     5401 agcggtgagg gtggtcgagc tgatcgccga gttgaagatc accgagaacc agtcggggaa
     5461 gccggcgtag aagcccgggt gcatgagcgg cagcaggccg acgcccagtg agcccgccac
     5521 gatcatcagg ttgaggttgt tgcggtagtc caccttcgcc agggtcgcga tgccactggc
     5581 cgcgaccgac ccgaacagga agatgcccac cccgccgagc acgggcgtcg ggacggcggc
     5641 gatgactcgc cccagcaccg gcagcacgcc cagcccgatc aggatcacgc cgccggtcgc
     5701 caccacgaag cggctcttca ctccggtgat cgcgacgagt cccacgttct gcgcgaacgc
     5761 gctctgcgtg aacgagttga acaccgggga cacggcgctg gcgaccatgt cggcccgaag
     5821 gccgtcggcg atgcgcttgg cgtccgtctt cgtgccgacc acctcgctga cggccaggat
     5881 gtcggcggtg gtctcggtga aagtcaccag gatcacgatc atcatcgaca ggatcgacgc
     5941 cacgtcgaag gtcggcagcc cgaacgcgaa cggcgtcggc agcgcgaaga cgggaccgtc
     6001 cgccacgccg gagaaatccg cggcgcccag gaacacggcc aggatcgtgc cgatgcccag
     6061 cgacagcagc acgctcaggc gcgcgatcga gggcgtgccg agcttggtca gcacgatgat
     6121 gatcacgacg gtggcggcgg ccaggcccac gttgcctgga gacccgaacg ccggatcctt
     6181 cgcatccggc cccatcgccc acccggcggc caccggcatg agcgacagtc cgatcgaggt
     6241 gatcacggtg ccggtgacga cgggagggaa gaagcgcagc accatcgcga acagcgggac
     6301 gagcaggatg ccgatgagtg acgacacgat gacggcgccc atcaccgctt ggatgccgcc
     6361 atcgccctgg atcgcgagca tggtcgcgat gcccgagaac gagacgccct gcacgagcgg
     6421 gagccgggag ccgaagaacg ggatgccgag ggtctgcagc agcgtggcga ggccgcccat
     6481 gaacagggag caggccacca gcatcccgag atcggcctgc gacatccccg ccgcctgacc
     6541 catcaccagg ggcggtgcga tgatgccgcc gtacatggtg agcacgtgct ggaagccgta
     6601 ggcgatgttc gcgcgccagc cgagcttggc gtcttcgggg cggatggagg aggatgcggt
     6661 cgcgctcatg ccctcgggtc tctcgggagt ggggaggtga ccagggtatc gcggcacgct
     6721 cgcgccgccg ctccgcgcgt gcgaggatgg ccagcgccta ccagccccca cgatcggagt
     6781 caccgtgccc gttctcgaga gggaacacgc gctgtctgcg gtcgtgctgg tcgagggccg
     6841 gagcgaccgc ctggcggtcg aggccctcgc gcgtcgccgt gcgaagtcgc cggcaggggc
     6901 cggcccgctc gacggagtgg agctgcgaga gctcgacggc atcacgaacc tgcgcaaggc
     6961 gctgaccgca ctgcgaggca cgtccgctcg cgtcctcggt ctgtacgacg cggcggaacg
     7021 gcgatacgtg cagcgggtcc tcgcaggcct ggggatgctc gacgatgcag gacggggtgc
     7081 gcagggcacc cccgacatgg acccgctcga gcccctcggc ttcttcggct gcgagcgcga
     7141 cctggaagac gaggtcatcc gcgccgccgg agccgagctg gttctcgagg ccctcgatcc
     7201 ccgggagcgg gcacggttcc gccggttcca gcggcagccg gcgcagcgcg tgcgcagcat
     7261 cgaagagcag ctgcacaggt tcgcgggcac cgccgccggc cgcaagtccc gcttcgcggc
     7321 ggatgtcatc gatgcggtgc cgctggagcg gacgcccgcg acgctcaacc ggatgctcga
     7381 tcgcgtgggt gatcgctgag gggtccgggc agggcatcca cccaaacctg agtcgaccta
     7441 tatcaacttt cttgacagca tccggcgtcg cggttacact tgagtcatcg cgactcaggt
     7501 tttcttgagt cgcccacgaa ctcactcaac ttcaccgctt caacaacaag gagaatcaca
     7561 tggcacgtgc tgtcggaatc gacctcggaa ccaccaactc cgtcgtcagc gtcctcgagg
     7621 gcggcgagcc caaggtcatc gccaacgccg agggcttccg caccaccccc tcggtggtcg
     7681 cattcaccaa ggacggcgag gtgctggtcg gcgagaccgc caagcgccag gccgtcacca
     7741 acgtcgaccg caccatctcg tcggtcaagc gccacatggg caccgactgg tcgttcgatg
     7801 tggacggcaa gaagtggacg ccgcaggaga tctccgcgcg catcctgatg aagctcaagc
     7861 gcgacgccga ggcgtacctc ggtgacaccg tgacggatgc tgtcatcacc gtccccgcgt
     7921 acttcaacga cgccgagcgc caggccacca aggaggccgg tgagatctcc ggcctgaacg
     7981 tgctgcgcat catcaacgag cccaccgccg ctgctctggc ctacggcctg gacaagggca
     8041 aggaggacga gctcatcctc gtcttcgacc tcggtggcgg taccttcgac gtctccctcc
     8101 tcgaggtggg caaggacgac gacttctcca ccatccaggt gcgcgccacc gccggtgaca
     8161 accgcctcgg tggcgacgac tgggaccagc gcgtcgtcga ttacctgatc aagcagttca
     8221 aggactcgac cggcgtcgac gtctcgggtg acaagatcgc cctgcagcgt ctgaaggagg
     8281 ctgcggagca ggccaagaag gagctgtcga gctcgaccag cacctcggtc aacctgccgt
     8341 acctgtcgct caccgagtcg ggccccgtct ccctctccga gacgctcacg cgtgccaagt
     8401 tcgaggacct caccaaggac ctgctcgacc gcaccaagaa gccgttcgag gatgtcatcc
     8461 gcgaggcgaa catcaaggtc agcgacatcg accacatcgt gctcgtcggt ggatcgaccc
     8521 gtatgccggc cgtcgccgag ctcgtcaagc gcgagaccgg caaggaagcc aacaagggcg
     8581 tcaacccgga cgaggtcgtc gccgtcggcg ccgccctgca ggccggtgtc ctgaagggcg
     8641 agcgcaagga cgtcctgctg atcgacgtca ccccgctgag cctcggcatc gagaccaagg
     8701 gcggtcgcat gaccaagctc atcgaccgca acaccgccat cccgaccaag cgcagcgaga
     8761 ccttcaccac cgccgacgac aaccagccgt cggtggcgat ccaggtcttc cagggcgagc
     8821 gcgagttcac ccgcgacaac aagccgctcg gcaccttcga gctgaccggc atcgccccgg
     8881 ctccccgcgg cattccgcag atcgaggtca ccttcgacat cgacgccaac ggcatcgtgc
     8941 acgtgtccgc caaggacaag ggcaccggca aggagcagtc gatgaccatc accggtggct
     9001 cgtcgctgtc gaaggacgac atcgagcgca tggtgcgcga ggccgaggag cacgccgccg
     9061 aggacaagaa gcgcgccgag gccctcgagc agcgcaacca ggccgagtcg ctcgcgtact
     9121 cgatcgagaa gctcatcaag gagaacgagg acaagctgcc cgaggacgtc aagtccgagg
     9181 tgcagggcga cgtcgacgct ctgaagacgg ctctcgccgg cgaggacgac gacgcggtca
     9241 aggccgcgtt cgacaagctc aacgcgtcgc agggcaagtt gggcgaggcc atctacgctc
     9301 agtcccaggc ggatgccgca cccggcgccg ccgctggtga cgcaggcgcc tccgccgacg
     9361 ccgggtccga tgaggacgtg gtggacgccg aggtcgtgga cgacgaggac gagagcaagt
     9421 aaccatgacg gacaagaact tcgacgaaaa cgacgaggtt cagcctgagg gtgcggggtc
     9481 ggatgctgcg gcatccggcc ccgagccgca ggtgaaccag gactccgccg aggcctccgc
     9541 agccgagggc tcggacgacg acttcacggt ggacgacatc ctgaacgccg cgcagacgcc
     9601 cgaggcgtcc agcgaggacg gctcggatgc cggcatcgtg gatgccgagc acgccctgct
     9661 caacgacctg aagcgcctga ccgcggagta cgccaactac cggcgccgca ccgaggagca
     9721 gcgccatgtc gagatcgccc gcgccaaggg cgaggtggcc aagggcctgc tccccgtgct
     9781 ggacgacctc gatcgcgcgc agcagcacgg cgatctggag gagggctctc ccttcgccgt
     9841 gatctccgag aagctgcgcg gcgtggtcga gaagctcggc gtcgtcgcct acggcgagaa
     9901 gggcgacgag ttcgacccgc agcagcacga ggcgatcttc cagcagccca cccctggtgc
     9961 caccacctcg accatcctcg aggtcgtcga ggtcggctac cgcctgggcg atgtcgagct
    10021 gcgtccggcg aaggtcgtcg tcgccgttcc cgcggagtag gtgatcgatg gccagtcagg
    10081 attggttcga caaggacttc tacaagacgc tcggcgtctc gaaggacatc tccgatgcgg
    10141 aactgaagaa gacgtaccgc aagctcgcgc ggaagtacca cccggactcc aacccgggtg
    10201 acgctgccgc cgaggcgaag ttcaaggaga tcagcgaggc gtacagcgtg ctcagcgatg
    10261 ccgagcagcg tcgcgagtac gacgagatcc gcgcgatggg ctcggggacg cgtttcaccg
    10321 cgcccggcgg cggtggcgcg ggcggcttcg aggacgtgtt cagccgattc gggcagggcg
    10381 gcggccgtca ggccgacttc gacgacatct tctcgatgtt caaccagggc ggcggctcgt
    10441 tcggcggagg ccggttcggg cagtcgtcgg gcggattccg tggcttcggc ggcccccaga
    10501 agggcgccga cgtcacggcc cgcaccacgc tggacttcgt cacggccgcg aagggcgaga
    10561 ccatcaccct gcaggccgag gacggcaagc ccttcaagat caaggtgccg gcgggtgtcg
    10621 ccgacggcca gaagatccgt ctgcgcggcc gtggtcgccc ctcgcccgac ggcggggagc
    10681 ccggcgacat cgtcgtgcag gtcgctgtgc gcccgcaccc ggtgttcacc cgcgagggac
    10741 tgaacctgcg gctgaccgtg cccgtcacgt tcaccgaggc gactctcggc gcgaccatcg
    10801 aggtgcccac cctgggcggc gagacggtca agctgcgggt cgctccgggt acgccctccg
    10861 ggcgcgtgct gcgcgtcaag ggccggggca tcgcctcggc gaagaagggc acgggcgacc
    10921 tgctggcaga actgcaggtg gccgtcccct cgcacctcga cgacgccgcg aaggaggcgc
    10981 tgcagcgctt ccatgagctc gagccgacgg agaacccgcg ggccgagatg atggcgaagg
    11041 ccaaggcctg acatggatgc cgagtccccc gtcttcgcga tcgcggtcgc tgccgagctg
    11101 gccggcatgc acccccagac gctgcggcag tacgaccgca tcggcctggt ggtgcccggc
    11161 cgcacgcgcg gcggctcgcg gcgctactcg atgcgcgaca tcgagcagct ccgcgaggtc
    11221 gcgcagctgt caagcgaggg gatgagcctc ccggcgatcg cccggctgct cgacctggag
    11281 gacgaggtgc ggatgctgcg ctcccgcgtc accgagctcg agggcgctct gcgcgccgag
    11341 cgggagagcc gccccggcgt tcgcgtcttc gcggccgggg cgaccgggtc gatcgtctcg
    11401 ctggcatcag gccggcgcat ccgtcgctcc accgaggtgg tgctgtggca tccgcgcact
    11461 gtgtcgacct cggagcattc ggacggaacc gcctccgacc ctcccctccc cggtacggac
    11521 tgacggcgga tctcactgct ggggcgtgaa gctcctgcgc agcacccaga cgttccccga
    11581 cggagcgggg tggtgctcga gcacgtcgac cgacgcacga gccagcgcga gtccgcgccc
    11641 gctctccgcc tcagcgtcgg gcatcgcggc agcggccagt tcgatcgtca cgggtgcggc
    11701 cgtgtcctgg atgagggcgt gcagagtacc cggcgtcacc cgtgcgctga cgctcatcgt
    11761 cacgtgaccc tcggaatgcg tgacgacgtt cgtcgagatc tcggcgatcg ccaggcggaa
    11821 caggctctgg tcctcgccgg gcacttccgg tgccgtgatc cacaggccgt cgagagcgtc
    11881 gaggacgcgt tcgacgagcg cgaggtcggc tgcgccctcg acctggatcg agcgcgtctc
    11941 agcgtccatc gaacgccgat tccggcgtct cgtgattccg cagcacgcga tcgaggttgg
    12001 tgaggcgcag cacggtgcgc accgagtcct ggacggccgc gacgcgcagg tcgccgccag
    12061 ccaccctcgc gctcttgaga cctcccacga gagcgcccag cccggtcgaa tcgacgaact
    12121 cggtcctcga catgtcgatg acgatgcggt tcgacccggc atcgatcagt tcattgacgg
    12181 cgttacgcag ctgcggtgcg cccgtggcag tcagccgccc gacgatgtcg atgacggcgt
    12241 agtggtcgcg gatggcggtg gtgaggttca tacgtctcct tcggatgggc gtgcgaccgg
    12301 ccccctgtag cgggctgctc cgatgatcac gctgagcacg accagatcgt aagcgaccca
    12361 gatcgcgttc acgagcgtgc ctgtcccgtc ggcggtgccg acgatgaccc ggatcacgcc
    12421 gatgacgagt gcgacggcga gcacgaccat cgcggtgaac tgcggccaga tcgctcccca
    12481 gggcacaccg cgatcgtcgc ggccgtcctt cttcgtcacg gcgaagccga gcggacggcc
    12541 cagcacgacg tttgcgaatg ccgtccagca ggcccggatc cacaccggga agagcgcgag
    12601 tgagtactgc tggccgcgcc atgtccgcat cccccgagcg atgacgatga acagcaactg
    12661 gttcgccgcg aacgcaggca ggaagcgcgc gaagaagtcg acgctccagg ccttcaccgg
    12721 cagcactccg aagagcaggt agatcacggg ggccgcgagg tacacgatgg cggcgaatcc
    12781 ggacagatag ctccacatcg ttccgaagta catcagtcgc tgcccgatcg acaggccgcg
    12841 cttcagcagc gggttgtccc gaagcatcac ctgcagggtt ccctgcgccc agcgcagacg
    12901 ctgggtgagc atcgtcctca gatcctcggg ggcgaggccc cgcgcaagga tctcatggtg
    12961 atagacgctg cgccagccga gcgcgtgcaa ctgcatcgcg gtggccatgt cctcggtgac
    13021 tgagatcgtc gccaggggga ggatgggctg ggcctcgtcc ccgcggtcga tgcgcagcgc
    13081 gtccagcagc gcgcgcaccg cgtcaatggc gccgagcggc gacaggtcgg ccatcgccag
    13141 accgtcgagg gccttgtcat cgacgacgac cgcgtccagc tcggcgtcgc gcgcgaccgg
    13201 aagccgcgcg atcacgtcga gatccgcccg gatcgtagcg aagtcgcggg cgacgagcgc
    13261 ctgggacgcc cgttccacgg ccgcccgaac gtcgaacgtg gcctccgcca caggttcgcc
    13321 ggcgttcact gcggaacgcg ccttctcgat gacggaacgc agctgcgtga gctcggcggc
    13381 cagcgcgggg tccttctgtc ggcgtgcctc gcggccggca agcgcagcgg ccacgcgcag
    13441 ggcgcgcgct gtcgaggtcg tgacatctcg gacatagccg acgatgccga gctgcatcag
    13501 cgactcacgt cgcagcaccg cgttcgagcc gcagaagaac gcggcgttcc agccgtcctt
    13561 gccctgctgg atcgggccgt agaagagcgg cgcctgactc ccgagcgggt ccgcgtcgtc
    13621 gacgttcgag aaccactgcg gcgtctggac gagagcaacc tccggatcgt cggcgaaata
    13681 ccccagggtc cgatgcagga tgagcgggtc cggtatctga tccgcgtcca ggatcagcat
    13741 gaactcgccg tcggtctgga acagcgcgct gttcagattc ccggccttgg cgtgacgggg
    13801 cttgcccgtc cagtcctccg agcgtgtcag gtagccgatg ccggcatcgc gcgcggcctg
    13861 ttcgagatcc tgccgtgatc cgtcgtcgag gatccacgtg gaatgcgggt acgcgatcgc
    13921 cttcgccgcg cgcgcggtgg tcatcaccat ctctatcggc tcgttgtaag tcgtgatgaa
    13981 cacatccgct gttccccgcg gcggcgagga gggacccgaa cgctctcggg cacgccacat
    14041 cgtgaggcag aacagcagcg tgtcgatcag gctgtatgtc tccgcgatca ccagcggcac
    14101 ggcgatccac catgcactcc agttcacgga ctccagccag cgccatgcca cgtaattgac
    14161 gcccagcaac gccgacagca cggcaagcac gcggataagc gcgaaacggg ccttcatcgg
    14221 gtcctgctca caaccacgac cgtgacatcg tcggtcgctt gtgctccgcc agcaagccgc
    14281 agggcctcct gaaccgctcc cttcggacct gccgtgcgca gcacccgctc gacgtgaccg
    14341 aacggatcat cggcgtcgag cacatcgagc agaccgtcgc tgcagcagat gaagtggtca
    14401 ccgggccgga cttcgacgaa atccgaacgg cgcggctcgg tgaaggccat ccccattccg
    14461 agcggcagcc cagtcgaacg cagcgattgc caggagccgt cggcccggag aacgaaagcc
    14521 aggccgtggc ccgcgtcgac gtattcgagg gagccggtgt cgagcttcag ctcggcatgc
    14581 acggcggtaa cgaacatcgt ccgatcaccg agatcctctt cgagcaggcg atccgcctcg
    14641 tcgatcgccg cggtgagcgc gcggcgcgga tccgtgcgca gcgaggccct gaccccggct
    14701 gcaaccagtg ccggaccgat tcccttgccc atcgcatcgg ccagtgtgat gcgcagggcg
    14761 ccgtgctgca gctcgaggtg gtagaagtct ccagcgagcc gcccgcggga gacgctcccg
    14821 gccgcgatct cgtagccatc cggggaaggc aggacggacg gctgcatgga ccgctgcagc
    14881 acttctgcgt gatccacctc ctggtccttc gcgagctctg tctgcaccca gaaggcgagg
    14941 tcgcgcagca gatcgcgctg cgcgacggtg agtgtatgcg gcaccgtgtc cagtacgcac
    15001 agcgtgccca cgggttcacc gcccggggcg tgcaggggat gcccggcgta gaagcggagg
    15061 tggggatcgc cgaccacgaa cgggttctca gcgaactcgg cggtggcgcg ggcgtcctcg
    15121 atcaccaccg tgcggttctg gcggaccgtg tagtcgcaga aggagtcctc acgaggggct
    15181 tcgtcgccgc cgaggccgat ctgagacttg cgccactgcc ggtcgtgatc cagcagcgag
    15241 acgctcacca tcggcacgcc gaacatctcc ctggccagcc gtgcgacgcg atcaactcgt
    15301 tcgtctggct gcgtgtcgag gactccgagt tcctcgagtg ccagctgacg tcggcgctca
    15361 tcgaacatca ccgtcccccc tccgatcgat gctctacata tcaggcagaa cgcccgcgac
    15421 cgatatatta ggcagaaggc ccgcgaacgt cacagcccca gcacggtctt cgcgatcagg
    15481 aagtagatca ccagaccggt cgcgtcgacg aacgtcgtga tgaacgggtt ggagaacaca
    15541 gccgggtcga cgcggatcgc gcgggccgcc aggggcatca tcccgcccac ggatgccgcg
    15601 accgtgcaca ccgcgaccag ggtgaggccg atcaccaggc cgacgtgcca ttcgtagatc
    15661 aggccagcga tcacgaatcc cagcgtgccc agtagcagcc ccaggcagaa tccgaccctg
    15721 atctcgcggg agagcacgcg cagcacgtcg cgcgggctga cctcgccgag cgcgagtgag
    15781 cgcgtcaccg tggtcgccgc ctggttgccc gtgttgccgc ccgtgccgat caggagcggc
    15841 acgaacagtg cgagcacggt catctgcgcg agcgtcgcct cgaacgtgtc cagcacctgc
    15901 acggtcagcg tcgcaccgac ggcgagcacg agcagccaca cgaccctcga gcgcaccagc
    15961 tgacggatgg gcgtccccag gtacgagcgg cgcagcggct ccacgccgcc ctggcgcgcg
    16021 gcatcctcgc tctcctcctg cttgaggatg cgcgccgcgt cgtcgatggt gaggatgccc
    16081 accagccgct gctcgccgtc cacgatcgga agggccagca ggctgtagtc ggtggcgcgg
    16141 cgggccgcgg tctcggcgtt ctccgtcacc tcggcgacgt gcggttcgct catcacgtcg
    16201 gagatggcgg cgtcctcatc ggccgacatc aggtcgcgca ggctgacgac ccccaccacg
    16261 cgccgggcgt cggtcacggg cacggtgtac acggtctcgg cgctgtcgag cgcggcgcgg
    16321 atgcgcgcga acgtctcgcg gacggtcagc tgcggatgcg tcgtgacgaa ctccgggctc
    16381 atgcggcgcc cgatggagtc ctgcgggtag ccgagcatcc cggaggtgag gtcgcgctcc
    16441 tgccgagtga gaccgcgcag cagctgggga gccaccgacg ccggcagctc gtcgagcagc
    16501 cagacccggt catccgggtc catctcggcg aacagtgccg tgatctcgac gtttcgcagg
    16561 gcctgcacca ggtcgccacg ggtgccgggg gagagggcct cgaacacctc gagggcgcgg
    16621 ctcttggcga gcaggcggta cacgatcgcg gcgtcgtcga tggggagccg gtcgacgagc
    16681 tcgacgacct cgggaacggc gagaggggtc agcacggcgg atgcgccggg caggtcaccc
    16741 tgggcgatga ggcgacggat ctcgtcggcg gcctcggaga cggtctgaag ctgggtagga
    16801 gtcatgggcg tgccttagga aggcaccgcc acactgagcg gggttacggg aacgccgtgg
    16861 cgcgcgtcgc agcagacgcc gccgctcagc cgagcggtgc ggaagcggag gggatgacgt
    16921 gtctgcgact gtgactgtca ctcgacatga tgaaccacct cgcttcctgg tgctcgggga
    16981 aggattcctc gtcgatccta gaccctttga cggaggatgt accctgtcaa cagtcaacaa
    17041 ggggagtgcc ggtgtcggac acagtcagaa gaggcgagtc tctgggcgcg cagatcgcga
    17101 acgtgctgcg gcagcgcatc gtccgcggcg tgctggctcc gggggagcgg atcaccgagg
    17161 aggcgctcgc cgaggagttc gacgtcagcc gggggccggt gcgcgatgcg ctcacccagc
    17221 tcagtttcga gaagctggtg cgcatccagc ggccccgggg tgtcttcgtc gtcgggctga
    17281 ccggcgaaga cgtcgatgag ctctacagcc tgcgtggtgc gctcgagcag ctcgcgctgt
    17341 cgcgggcgat ccgcgtgtcg gacgacgccc gctggcggac gatggcggac gccgttcagc
    17401 ggatggcgga cgccgcggat gccggtgacc atgccgcctt cgtggaggcc gacctcgact
    17461 tccactcgca gatctacgtg ctcgccgacc atgcccgcct cgagggtgcc tggaagcagt
    17521 acctgccgac gttcacggct ctgctcgacg tcacgatcaa ccacgacgac gatctgcacg
    17581 actccgcagg cgaccacgtc gtgctgatgg acgtcatgcg cagcggcgat gccgcgcgcg
    17641 cggccgaggt gctcacggcg cacctggacg gtgcccgcga ccgcatgctc agcgaactcc
    17701 cgcagcagac accctgatcc ggccccgcgg tctgcgcggg catcgtctcg ggtgttgact
    17761 gttaacagtg aacactgcta cgctcgccag cgtcgacaac acggtcgacc agcaccgagg
    17821 agcaaagatg ccccgcatcc gcatgtcccg agcggccgca gccatcgtcg ccgtcaccgc
    17881 aaccgcactc gtcctggcgg gctgcaccaa ggtcgaagag ggaggtgccg agtcggcctt
    17941 ccccgagaag gacattcgcc tgatcatcca ggcgaacccg ggtggcggct ccgacctctc
    18001 ctcgcgcgca ctcgccaccg agctcgagaa gatcctcgac gtgagcgtcg tccctgagaa
    18061 catgcccggc gctgccggcg ccctcgccat ggagtacgtc ggcagccaga aggccgacgg
    18121 ctacgtcatc ggcttcgcgc ccgtcgagat cgcgatgctc aacaccaccc agggcgccaa
    18181 ggtgctgccc gagaactacg atctgctcgg ccaggtcatg ctcgcaccgg gcgtgatcac
    18241 cgtcggcgcg aacagcggca tcgactcgct cgaagcgctc gtcgccaagg cgaagtccgg
    18301 tgccgtgacg gtcgcgaact ccggtgccgg ctcgatctgg gaggccgcga ccgtcggtct
    18361 cgccgacgcc accggcgccc agttctcgcc cgtcccgtac gacggcggcg ccaccgccgt
    18421 cggcgctgcc gcatccggcg agacggtcgc cgcggtctcg ggcctcggcg aggcgctcgc
    18481 gcagggcgac gccgtgaaga tcctcgctgt catgcacgat gagcgccacc ccgacgcgaa
    18541 ggatgtcccc accgtcgagg aggccatcgg cgagaaggtc gagttcggcg gctggggcgg
    18601 catctacacc gccaagggcc tgccggacga cgtgcacaag accctcgagg atgccgtgaa
    18661 gaaggccgtc gagtccgacg gctaccagaa gttccagacg gaggccggaa acctggtcgt
    18721 gtaccgcgac tcggccgagt ggacctcatt cgtgaaggac cagttcaccc tgttcaagga
    18781 cttgctgggc tgagtgcccc gggaccgggt ccacgggccc ggtcccggcc tctcgagagg
    18841 acacccatgt cgaccaccga cacccccgtc gaggacgagt accagagcgc ggcaccgtcg
    18901 cgcggactcg agatcgcctt cgccgtcgtc gcgctcgcgt tcgccgccgg ctacctgttc
    18961 ctggccacgc agatcccgct gcgccgcgag gccgcggccg ggcagatgga tgcccggttc
    19021 tggcccaccg tgatcggtgt cgcggccgtc gccctcgccg tcatcctgct cgtcattgcg
    19081 atctcgcggc ccgcgccgag ccgcgaggac gtcgagcgca tccagcccgg cggcgtgctg
    19141 cgcatggccg cgacgctggc gatcgcctgc gtcttcgtcg cgctctggtc gctggggtcg
    19201 gtggtgctga tgggctaccg catcgagatc ttcccgatcg ccgcaggcct gctgatggcc
    19261 tccctgatgc tcgtctacgg ccaccgccgc tggttgagcc tgatcatcta caccgtcgcg
    19321 gtcacggcgt tcgtctacgt cgtgttcggc atgctgctga ggattcccct gtgaacgctt
    19381 tcgccgaggg cctgaacgcc ctgctggacg tccagatcct gctgaacatg ggcgtcggtc
    19441 tcgtactcgg cttcatggtc ggcgccttcc ccggcatcac ggccaccatg gccgtcgccc
    19501 tcgcggcggg cttcaccatg acgctcgagc ccgtgcaggg cctggctgtg atgctcacca
    19561 tctacgtcgc cgccaacttc ggcgaccggg tgccgtccat cctgatcaac accccgggca
    19621 cgcccgcctc catcgccacc accctcgacg ggtatccgat ggccaagcag ggcaaggcgg
    19681 gactcgccct gactgtctcc gcgatcgtct cggccgtcgg catcctggca tccctcgtgc
    19741 tgttctcggt cgccgcggtc cccatcgcca gcttcgcccg cgactacttc cgctctcccg
    19801 agctgttcgc cctggtgatc ttcggcatcg ccatcatgat cggcatctcg tccaagtcga
    19861 tggtcaaggg catcctcgcc ggtctcttcg gcctgatgct gggcacggta gggacctatg
    19921 ccgccacggc cgaccagcgc ttcaccttcg gatcgctgga gctggtggag ggcgtgaact
    19981 tcatcgcagt gatcatcggc ctcttcggca tcgcggagct cttcgaccag ctgctcaccc
    20041 accgcaagaa ccgggtgcgg ccgatctcca gcctcggccg ctggtggccg acctggggcg
    20101 agatgaagca gaccggtcgt gccaccgcga tctcgggtgg cgtgggtctc ggagtcggcc
    20161 tgatccccgc ggccggcggt gacatcgccg ggctcatcgg ctgggagcag gcgcgccgcg
    20221 catccaagca tccggagcgc ttcggcaagg gctcgatcga gggcgtcgcg gcatccgaca
    20281 cggcctccag cgcgacgctc ggcgggtcgc tcaccacgac catggcgctg ggcatcccgg
    20341 gcgactcggt catggcggtg atgatcggct cgatgatcat ctggggcatc acccccggcc
    20401 cgacgctctt caccaaccgg cccgacctgg tggtctccat cgtcggcatc atgctcctcg
    20461 ccacgctgct ctcgctcggg ctgagcctgg tgcgcatgaa gggcatggtc aagctgctcg
    20521 acgtgccgca gcagtacctg tggagtggca tcctgatctt ctgcatcatc ggcacctacg
    20581 ccacctccaa cagcctgtcg accgtgatca cgatgctcgt gttcggcgtg gtcggcgtgc
    20641 tgctcaagcg gatgcaggtg ccggcgggcc cggtcgtcct cggcctgctg ctgggcccgc
    20701 tcgcggagga gaacctcgcc cgcacgatgg cgatcctgcc gaccaagccg ttcttctcgg
    20761 tggtcagccc catcgcgatc gcgctgctgg tgctcgccgt gctctccatc gccctgccgg
    20821 cgatccgtgc cgcccgcaag ccgcgcgagc agcgcgcctc gctcgaggag tcgatcctgc
    20881 cgacgtccag catcgagcag atcgagcacg cccgcgagga gctcgcgcag gaccccgaac
    20941 tgctcacgtc caccgtgcgc aacatcgaga agaagggcgc gcgtcgtgcg cgccgcaccg
    21001 acaaggagaa cgagaagtga cccgctacga catcctcacc gcgatcccca cggccttcgg
    21061 gcgcgatgga tcgctcgatc tggaggggtc gcgcgcgatc ttccgctacg tcgggaagtc
    21121 cggcaacgag ggcgcgttcg tcctcggcac gaccggcgag ttccccgccg tcgacctcga
    21181 cgagttcacc gctctcgtct cggcggcact ggacgagctg caggaccgga tgcgggtcgt
    21241 cgtgcacgtc ggacgcccca gcaccttcga ggccgtccga ctcacccgca tcgcccggga
    21301 gctcggcgcg accgagttcg cggcgctgac gccgtactac ctccgcgcct cggacgatgc
    21361 ggtgttcgac tacttccggc aggtgtcgga tgccgtgggc gacgggcggc tgtacgtgta
    21421 catctacccg gcacgcagcg ggaacccggt ctcgcccgag ctgctggtgc gcctcgcggg
    21481 gctgccgaac gtggtcggcg cgaaggtcag cgagctctcg ctcgacgaac tggccgccta
    21541 ccgcgccgcc gtgcccgcgg acttcgagct ctacacggga gccgaccgcg atctcatcgc
    21601 cgcggtcgac gccggcgcgc agggggtcgt ctcgggcgtc tcctccgtca cgcctcagcc
    21661 gttccgtgca ctggcggatg ccggccgctc cggggacgcc tcggcgatcg ccgccgcgca
    21721 ggcggccgtc gacgaggtcg tcgccctgat cggcggcgac atggcgcgca tgaaggaggg
    21781 ctatcgggtg ctgggcgtcg cggactccta ctgccggatg gcgctggacg agccgacgga
    21841 tgccgagcgc gaggccgtcg cgcgagtggt ggccgcatac gcctgaggcg gctccgcccc
    21901 tcgagaaacc accccgcgca tgagagatca tgcgcggggt ggtttctcat gtcgaacggg
    21961 tttctcaccg gcgtcagtcc agcggatcga cggcgaagcg gaagctcgcg ccctggcgga
    22021 agggctcgga tatctcgaag ttctcgaacg ccgggccgcc cggtatctcg gaggcactcg
    22081 cccagtacga ccatcgagcg ttgacgagca cctcgaggtc ggggtgcggg tcgtcgacgc
    22141 gccaggtctg tcgcgaccgc agccccacga accgccagtg ctccgccgtg tcgtcggcat
    22201 aagcgaccga ccacggctcc tcctcgtcgc cgatcgcgct gaccgccgcc gtctggccct
    22261 cccggcgcag ctcggccagt ccgaaccgcc cgtgctcggt gaagccggtg cccccgaacc
    22321 cgggccagag ctcggctgga gtgacggtcc ggtcggccag ctcgagccgg cgcagtcgcg
    22381 cccagttgcc catggtcgcg gtgagcacca ggcgctgcag cggtgcggat ccctcgttct
    22441 cgaatccggc cacttccacc tcgcgcgggc ggtccgcgcg gaatcgcacg cgcacccgca
    22501 cgtccgcccc gttgtcgaac cgctccaccc cgatccacac ggtcagcgtc tcgacgccgt
    22561 cgatcgtctc gaccacgccg cggctcggtg cgagcgggtc cctcggtgcg gcgagaccgt
    22621 cggggtcgtc accggaccag aaccgtttgc cgttctcgtc ggggtcgagt gaactcggct
    22681 ccagctcgga atatccccgt cggtcctcac ctcgcgggat gggctccacg gcgatgaagt
    22741 tcacgagccg ctcgcgcggg tgatccaggt acggggtgaa cacccgcagc agcccgcgag
    22801 ggcccgggat cggatggatg ccgatctgca tgccgtgcgc gtgcccccag cgcggctcgg
    22861 catccgcgac tcccgtcggt cgcagccaga tgccgtccgc agtcgccggc tccgcttccg
    22921 tgaagcgctc agtcaggcgc atcggatctc cttctgtctt gaaatcgatt tcgccatggt
    22981 atcgatttca gtagagtgtg gccagatcaa ggaggatcat gagccatctc acgtcagctc
    23041 agcgggcgct cgcgacggtc gtcgtcgcgg catccgctct cgcgctcggc gcctgctctg
    23101 cgagtgggcc gatcgcggtg gatccggacc cagacaagcc gttcgtgctc gcaggagtct
    23161 ccgacgtcac cgagatcgcg cagctcaccg gacccggcgc catgaacgac accgcctccg
    23221 tcggtgtggc gggcaccgat ctcgggtcga tggtgaacgt cggcgagcgg accttcttcc
    23281 tcttcggcga caccttcggc gagcgggatc ccgactcggt cggcggccag ggcgggttct
    23341 ggcgctcgaa tgtcgcggcg tggacgaccg acgacgatcc gaccgacggc atcaccttcg
    23401 acggctggca cgtggatgac gtcggcctgg ccacggcgct cgtcgaaggg gaccacgact
    23461 ccaacgacat cggcggggag gtgacgaaga tccccaccta cggattcacc gtcggtgacg
    23521 acatgtacgt ctcgtacatg tccgtgtccc actggggtga ccccggcgcc tgggatgcga
    23581 actactccgc gctgatcgca tcctccgacg gcggggacag ctggcatccg gtccccgggg
    23641 tgcgctggcc gggcgactcg aacttcgtcc agttcgcgac ggctcacgtc acggacggcg
    23701 gcaccgagta cgtgtacatc tggggcatcc gctccgggag gttcggcggc gtccagctca
    23761 tgcgcgtgcg gtccgagcgc gccgccgtgg aatcgcagtc gggctacgag tacttcgcgg
    23821 gagccgacgg ctcgcagccg cactggagtg cagacatgac ggacgcggcg accgtgctcg
    23881 agggcacgat cggcgagctc tcggtgatgt ggtcggccta tctcgaccgc tggatcatga
    23941 cctattcgga tgccgggaat gcgtacatcc gcgagggagc gacgccctgg ggtccctggg
    24001 gtgagccgat cgagctggcg tcggcgagcg actacccggg gctctactcg ccctatctga
    24061 acccgcggta cacctccgcc gacgggcgca tcgtgtactt cacgatgtcg ctctggggcc
    24121 cgtacaacgt cttctggttc tccgctcgac tcgatcgcag agtgccgtga gggagtccct
    24181 tgtgcttgaa atcgatttcg tctaacgtga cctctacccg gtcggcgcgg cccgttcgcc
    24241 cgcccgtgtc cctcggcacc agccgaacac agccccgatt cgacgacgat tcgtgttcct
    24301 gaggaggaac caatggcaat caacacttct cgcaagaggc ggatgatcct ctccgccctc
    24361 ggtgcggtca cagcggtcgg ccttctcgcc ggctgcggtt cgagcactcc cggccagggc
    24421 gccggcgaag gcgacggcgg cagcggcggc gccaccacgg tgcagttctg gcaccgcacc
    24481 ttcactccgg tggagaacgc ctggtacaag gacatcgtca agaagttcaa cgcctcgcag
    24541 gacaagatca aggtcgtcga caccgaggtt cccgccgacg cgtgggacca gaagatgaag
    24601 gcggcgcagg ctgcaggcaa ggctcccgac atctacacgc actccggcag catccaggac
    24661 gccgtcaacg ccggtcagct ctacgaactc aacaaggtcg tcgatcagtc caagctcgac
    24721 gagatcatcg atgcggcgaa gcccgtctcc gagatcggcg gcaagtacta cgcgtatccg
    24781 ctgctgctcg aggcgcagac cgtgctgttc tggaacaagg agatgctgac caaggccggc
    24841 gtcgatgcgg aggccggtcc caccacctgg gacgagctgc tcgcggactg cgccaagatc
    24901 cagccgacgc tcgccaaggg cacgtactgc atctccccgg cgtccgacgc cgtgaccttc
    24961 gcctggtcca cggtcggaca gcagtacaac ttctccggtc acaccgcgct gaccgacgat
    25021 tggaccaagc ccgacatcaa caacgacggc tacaagagcc tgatgctgaa gtacaaggag
    25081 ctctgggaca agggctacat ggccaagcag gcgctcgacg cgtacgtcgg cggcaaggac
    25141 ttcggcgaga agaaggtcgc cttcaaggtg tccggctcgt ggatgatgtc gcagatcggt
    25201 tccgactaca aggacatgct gaccaacacg ggcgtcggcg cgttcccgaa cgcctccggc
    25261 gcagacggtc gcacggcgac caccctgggc aacttcaagt gggtgatcga cgcgaagagc
    25321 aagaaccccg aggccgcggg caagttcctg gagtgggctc tcgccggtga caccgccaac
    25381 ctcgtgccgt tcttcgtgga cacccagttc accaaggtgc cggtccgcaa gtcggtgcag
    25441 gatgccgtcg ccaaggacga ggccgccgcg aaggcaccct ggtcgaaggt cgtcgtcgat
    25501 gacatcgccc cgaccgccat ccccgagccg acctacccgt gggacgtgtc cctcgccgtc
    25561 ggcaccgcga tggagtcggt gatgaagggc gctgccacgc cggatgccgc gatcgcgacg
    25621 gccgagaagg cgatccagac cgtgatcgat cgcgagaagc tgcccgagaa ggcgccgaag
    25681 aactgacatc cgagatccgg gagccggccg actggcgccg gctcccggaa ccatgccgac
    25741 aggacgggac caccgatgac caccgccgag aggggcgtgc ggcgccaaca gcgtcagcgc
    25801 catgaccgct accgctaccg ggacaccaag ctcgcgctgc tgatgctcgc gccggtgctg
    25861 atcctgctgg ggatcttcgt gatctggccg gccttctacg cggggttcct gtccttccag
    25921 gactggagct tctacaagga gcccgtgttc gtcgggttca agaacttcac cgatgtcctg
    25981 agcgacccgc tgttcatcgc cacgatcggc cgcggcttcg tgttcgtgct gatgaccgtg
    26041 ccgccgatgc tcgtgctggc gtacctgttc tccagcctgg tcgtcgcggt gtcgcgccgc
    26101 ttcgccagca tcctcaaggt cagcatctac gtcccgacca tcatctcggg cgtgatcacg
    26161 tcgatcatct tcgtgctgat ctacgactac tccggtggtc tgctcaacgc cttcctcggc
    26221 gctttcggca tcgatccgat cgcgtggatc ggcgatcccg cctgggcgct cctggcgatc
    26281 gcgatcccgg ccgtctggct gggcatgggc ctgacctcgc tcatcatggt cgccgcgatg
    26341 gtcgacatcc cgaccgagta ctacgaggcc gcggcgatgg agggcgccgg ctggtggcag
    26401 aagaccttct acatcaccat cccgcagatg aagaacgtct tcctctacct gctcgtgacc
    26461 gggttcgtcg cggcgatcca gcagttcgag ctccccctcg tcatgaccgg cggcgggccc
    26521 ctggattcga ccacactgcc gaacctgttc atcttcaacc acttccgcaa cgacatcaac
    26581 gtcggctact cgatcgccgc cgcactgctg ctgttcgtcg tgctgggctc cgtctcggcc
    26641 ctcgtgttca agttcgtcaa ctccgagagg ctggtcgact gatggccgtc atgcagggtg
    26701 agaccacccg tatcgtgctc gcgggcaagg aaccctccgg ccggtcgaag ctgccgcgga
    26761 cgtcgggcgg ctggctcgcg ctggccggaa aggtcgtcgt cggcctgatc ttcctcgtcg
    26821 ccgcgctctt cccgctcgcc tggatggtga tcgcgggctt caagtccaag accgaggtcg
    26881 tctcgacgcc gttccagttc ttccccgagg tgtggcgctg gcagaactac gcgcagatcc
    26941 tcgccgaccc gacgttcacg cgaacgatgc tgtggacgtt cctcggcgcc gtgctcttca
    27001 cggtgctgag cctgaccgtg aactctctgg cggcgtacgc gttcgcgcgc ctgaacttcc
    27061 ggttccagcg caccctgtgg gtcgcggtca tcacgaccat gttcatcccg ggcatgacaa
    27121 tcctgctgac gagcttcatc gtggtcaccc gcctgtcgat gctcgacacg ctcgcggtgc
    27181 tcgtgatccc cggggctgcg agcgcgggag cggtgttctt catccggcag ttctatctga
    27241 acatcccggc cagcctggag gaggcggcca tgctcgacgg atgcggccgc ttccagatct
    27301 tctggcgcat cttcctgccc ctgtcgaagc cgccgttcgt cgtgatgggg atcaccgcct
    27361 tcctggccta ctggaactcc tacgtgtggc cgatcatgac gatcacgacc cctgagcgct
    27421 tcgtggtgca gcagtatctc gccacgttcc gctccgagcg gagcaccgag ctcggcctgc
    27481 tgatggcggg atcggtcctc gccgcggctc cggtcgtgat cctcttcctc atcttccagc
    27541 gccagatcat cgggaacatc aagatggccg ggctcaagtg agcggagcac caggatgctg
    27601 ctgtcggtga cgggcgcgag cgggacgggc aagtcgaccg ccctcgatct gctgcgcgag
    27661 gtcgacttcg ggactcccgt gatctgcgtc gagttcgatt cgatcggcgt gcccgacggc
    27721 gcggacacgg cctggcgcca cgacgccacc gagcgttggg tccgcttcgc cctcgaggcc
    27781 cagtcgcggg gcgagcacgt gctgctgttc ggccaggtgc cgcccggtga gctgctcgcc
    27841 gtgccgtccg ccgagcagct cgacgggctc gcgatctgcg tgctggcggc gtcaccgcac
    27901 gcgcaggagg cacgcctgat cggccggggc gaaccagccg actcgctcgt gcaccacctg
    27961 cgtttcgggc agtggttccg aagccactcg gaggatccgc agcatgcgcc ggaggtcatc
    28021 cgcgtggaca cgccggtgcc gatggaatgg tcgcgctggg agacgctgag cggcgacgat
    28081 ccgcgctggc cggtgacggt gctggacacc gatgcactct cggccggaga ggtcgccgag
    28141 cggatcgagg catggacgag gaagaacctg gccgacgtcg agtcggccga accccgaggc
    28201 cgctgaaccc gacctcacga cgaaggagtc gagcatgacc gagcagtacc gccaaccatg
    28261 gatgagccgt cgcgcactgc tgcagggtgc cctcgctgcg ggggcggtgg gtgcggcatc
    28321 cgccgtcggc atcgcggccg caccggctgc gacggccgcc gggccgctga ccgtcactcg
    28381 gatgaaggtg ctggcgggca cccagacgag cgtgcagtac ggcgtcgggg cgaccgacct
    28441 gggcatcccg gcgcgcaccc ccgacgggcg cttgctcttc gtgttcgggg acacctgggc
    28501 cgaccacgtc ggcggcgcga actggcgctc acccgtggcc ctgtactcgt cgacgacgaa
    28561 tctgacagcg ggcgtgacgt tcaacgggtg cgccggcgcc ggaacggcga cgaccggcgt
    28621 ggccccgcag ctctggtatt acccgcatga cgcctacttc aagacggtca tcccctcgga
    28681 tgtcatcacc atcggatcgc ggatgtacct gcatgcgatc gtcaacggcc ccgagttcgg
    28741 catggtgcga tggaccgagc tgtgggcatc cgacgacagc ggaggcacct ggttccacac
    28801 cgggctgaag ttcccggcgg acatggcggg cgggcgcttc cagtgcatca cctggggttt
    28861 gggcaacgac gggtacgtgt acatctacgg gacggggttc cagcgcgaca agggaatcgt
    28921 gctgtaccgc gtggcatcga gccagatgac gcagctgtcc gcctatgagc cgtggggatt
    28981 cgcgaatggg tcgtggggct gggggaagcc cgccaccgag gtgctgccag gcaagttcgg
    29041 cgagatgtgc ctgcgaccgc tgggcggcaa gtggatcctc acgtggttca acgcggccga
    29101 ctaccggatc gacgcgatgg tgctgaacac gccgaccgac aacctctaca cggctgcgaa
    29161 gacgaccctg ctgtggggcg gcagctgggg gtcggaggat gctacgcacg tcgcgcagct
    29221 ctacggcggc tacatcatcc ccggatccac tctgggcgac ctgcatctga gcgtcagtca
    29281 gtggaacacc ggcaacagca gcgtctacca ctccgagcag ttccgggtgc aggggctggt
    29341 ctgaccacga cgaaagagcg cggacgatga tcgtcctccc cgagaagtac acgccgccac
    29401 cggcgggcgt caccccggcc gggttcctcg tgtggctcgt gcgcatgcag tggaccaccg
    29461 tgctgcaggg cgtcgtgtgc gacgtgatct ggctgctcgg cctcgcactc accccatggg
    29521 cgatcggccg cgcggtcgat gaaggtctgg tggcgggcga ctacggcgcg ttcctgcgtt
    29581 ggctgggcgt ggtggtgtgg ctgcagctgc agcactccct gatccagggc cttcgggaca
    29641 gagcgggttc gatcaactac gagcgcggag tcacccgcgt cgaccaggtg atcgtgcaca
    29701 ccgcgacgcg catcacgacc gcagccgacc gcaggctcgg agcgggttcg gtggtgacca
    29761 tggcctcgaa cgacgcctgg tggttcggct tcgtgccgat caacgtcggc acgctggtct
    29821 cggcgctcgc ctcgttcatc acggtttcag tgctgctgct gagggactcc gtgctgctcg
    29881 gagtgctcgt gatcgtggcg gtgccgtcgt tctcgatgct gcaggtgtcc ctggtgaacg
    29941 cgttgcgctc gcggcagagc gccgcgcggg aggccacgcg cgagatgaac tccgtcgccg
    30001 ccgacagtgt gcgcggcctt cgcgtgatcc gcggtatcgg cggcgaggac tggttcctcg
    30061 agcgcttccg tgaccgctcc gaccgggcgc gcgacgcgca gaagactgtg gcgggccctg
    30121 tctcgctggc ggatgccctg agtgtgttca tcgccggcgt gctcgtggtc gggctcacgt
    30181 ggatcggcgc cgcgatggtc gccagaggag agctgcgggt cggcgagctc gtcgccttct
    30241 acgggtacgc cggcttcatg gtgctgccgg tgacgctctt caaccaggtg atccgcgtgg
    30301 tcgtgcgcgg gatgatcgcc tcacgcgcga tcgtggagat gctcgacgtc gagtcgccgt
    30361 ggccggatgc cgcagaccga ccggctccgg atcggccgtc acagcggccg ggagcgctgc
    30421 acgacaagcg cacgggactg accgtgcgcg acggcgagca gctcgcggtc gtcgtgcgcg
    30481 actcccagga ggcgcaggcg ctggtcgacc ggatcgcccg gctggcgccc gacggcgagg
    30541 acgccgtggt gctggacgga cgtctgcaga gcagcatccc ggtccagagc gtgcgccgcc
    30601 gcatcgtggc atccgatccg gtccccttcc tgttcagcgg cactctccgg gagcttctcg
    30661 atccgtgggg ccggcacgac gacggcgaga tcctgcgggc ggtctccgcg gtggacgccg
    30721 ccgacatcct ggcaccgctc gacgaaggac tggacgtgcg ggtcggggag cgcggcatcg
    30781 agttctccgg cgggcagcgt cagcggctcg gcgccgcgcg ggcactgctc gccgacgcgc
    30841 ctgtcttgtt gctgcacgac ccgacctcgg cggtcgacgc ggcgacggag gagcggatgg
    30901 cgaccggcat ccgctcccac cgggcgggcc ggaccacggt gatcgtcacg accagcccgc
    30961 tggtgctcag cagcgccgac cgcgtggtgg tcatcgagga gtcgaggatc cgcatcgaag
    31021 ggacgcacga agagctcgtg cgcggcgacg ccgactaccg tcgagcggtg ctgcgggcgg
    31081 agggtgtgtc atgaccggtc atgctctgcc cagggccgat gcgcggcgag tgcgagccga
    31141 gctgcgcgcc atctggcagc agcaccgagc ccgcttcctg ctggtcatcc tgctcaacgg
    31201 cgccgccgcc gcaagcgggc tgctcccgcc acgactggtg gggctgatga tcgaccgcct
    31261 ccgagacggc gccgatgcgg gcgaggtcgc gctgtactgc ggcgcgatgc tcgcgggggt
    31321 gatcgcgcag agcctgttcg tgctcaccgc cgcgcgaacc gcgtggaagc tcggcgagga
    31381 cgtgttcgct ggcctccgtc tcgcgtttct gagcgacgct ctcgccgttc cgatgggcgt
    31441 ggtcgagtcg gccggcaccg gcgagctcgt gacccgcacg actcaggaca tcaacgccgt
    31501 ctccgagact gtgcggtggg tgctccccgg cgtgctggtg tcggcgctca ccgtcgtgct
    31561 cacactgatc gcggccgtgg tcgcctctcc gctgatcaca ccggtgttcc tgcttgcggt
    31621 gccgctgctc gtgctcatga tgcgctggta cgtgaagcgc gctccgcgcg tgtaccagcg
    31681 gatgggtgac agttacggtc cggtgttcgc cacgatggac gagacggcca acggcgcgcg
    31741 cgccgtcgag gcactcagcc tgcaggaggc cagacaacgt tcgctggcgt cggcgctcgc
    31801 cgggcactgg accgcggcgg tgggccgcat ccgcctgcgc cagacgatgc tcccatggtc
    31861 gaatctcgcg ttcgcgatcc cggtcttctg ctcactggcc tggggcggct ggctcgccgt
    31921 gcacggcgag gtgagcgtgg ggacggtcgt gaccgtgacg ctgtacgcgt ccgcgctcgt
    31981 cgcgccgctg gagtccctca tcgactggac cgacgagctg cagcgcagct tcgtctcgtt
    32041 cgcgcggatc ctcggggtcg gggagatcgt cgatcgctcg ccgcacgcgg gtcagcagcc
    32101 tgcatcgacc gacgtcgcgc tgcacggcgt cggcttcgcc tacaccgagg ggcgcgaggt
    32161 cgtgcacgac gtgtcgctgc gggtcgtgcc gggggagcgg ctcgcggtcg tcggaccctc
//
LOCUS       DATMSX010000297         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_137103,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000297 DATMSX010000000
VERSION     DATMSX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137103"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN041_09060"
     CDS             complement(<1..186)
                     /locus_tag="VN041_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29228.1"
                     /translation="MIERGAGDPTSLRAEAEDDQGLVVEQTPWGPAHRLQVPLKITGI
                     PLQWTLPAAELGAHRARW"
     gene            complement(183..1481)
                     /gene="rlmN"
                     /locus_tag="VN041_09065"
     CDS             complement(183..1481)
                     /gene="rlmN"
                     /locus_tag="VN041_09065"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWU29229.1"
                     /translation="MPASDKTPPARSTPVRTTGPARTQVRPATEGWTQKKDAEGRPLL
                     QFASPKRGKPPVHLADMTPEERIAKVKELGLPGFRAKQLSTHYFTHYTSDPAHMTDLP
                     AEQREELVGGMLPPLMTEVRRLTTDRGDTIKFLWRLHDGALVESVLMRYPGRITLCVS
                     SQAGCGMNCPFCATGQAGLTRNMSTAEIVEQIVRANRAIADGELGGKRASDHSMERVS
                     NIVFMGMGEPLANYKRVMDAVRVMVAPQPNGLGMSARGITVSTVGLVPAIRKLADENI
                     PVTFALSLHAPDDQLRDELIPVNSRWKVDEALDAAREYFEKTGRRVSIEYALIKDMND
                     HAWRADLLAEKLNARGRGWVHVNPIPLNPTPGSIWTSSDVDVQNEFVRRLNDAGIPTT
                     LRDTRGKEIDGACGQLVATTEDEAAAAARHACHWRARRSC"
     gene            1561..1998
                     /locus_tag="VN041_09070"
     CDS             1561..1998
                     /locus_tag="VN041_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HWU29230.1"
                     /translation="MEPWMWIVAVVVLLVLVPWATKGMRPKAPQPTASVAPRALSPAE
                     SAEIDALVAGDRKIAAIKRLRELQSLGLQEAKDAIDAWVPETTWTPAAAASASAPGDA
                     LTPAARAEITALIADDEKIRAIKRYREETGVGLKQAKDAVENW"
     gene            2057..>2346
                     /locus_tag="VN041_09075"
     CDS             2057..>2346
                     /locus_tag="VN041_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29231.1"
                     /translation="MTDRLSRARTAGVLAVLRAPSPESALEAADAIIRGGIPGIEVTY
                     TTPDAPAVIRELIARHGDDQLAGVDVVDAHLGPRILRLHQPTLDGIRAHGCQ"
BASE COUNT          336 a          812 c          826 g          372 t
ORIGIN      
        1 ccaccgggcc cgatgcgcgc ccaactccgc cgccggcaag gtccattgca ggggtattcc
       61 ggtgattttc aagggcacct gcaggcgatg ggccggtccc cacggcgtct gttcgaccac
      121 cagtccctga tcatcctcgg cctcggcccg caatgaggtc ggatcccccg cgccacgttc
      181 gatcaacagc ttcgccgtgc gcgccagtga caagcgtgcc gagccgccgc ggcggcctcg
      241 tcctcggtgg tcgcgaccag ctgaccgcag gccccgtcga tctccttgcc gcgggtgtcg
      301 cgcagggtgg tcgggatgcc ggcgtcgttc aggcggcgca cgaactcgtt ctgcacgtcg
      361 acatccgacg aggtccagat cgacccgggc gtggggttca gcgggatcgg gttcacgtgc
      421 acccagccgc ggccgcgcgc gttgagcttc tccgcgagga ggtcggcgcg ccaggcgtgg
      481 tcgttcatgt ccttgatcag cgcgtactcg atcgagacac ggcggccggt cttctcgaag
      541 tactcgcgcg cggcatccag cgcctcgtcg accttccacc gcgagttgac cgggatcagc
      601 tcgtcgcgca gctggtcgtc gggggcgtgc agcgagaggg cgaaggtcac cgggatgttc
      661 tcgtcggcga gcttgcggat cgccgggacc aggcccacgg tggacaccgt gatgccgcgg
      721 gcgctcatgc ccaggccgtt cggctgcggc gcgaccatga cgcgcacggc gtccatcacg
      781 cgcttgtagt tggccagcgg ctcacccatg cccatgaaca cgatgttcga gacccgctcc
      841 atggagtggt cgctcgcccg cttgccgccc agctcgccgt ccgcgatggc gcggttggcg
      901 cgcacgatct gctcgacgat ctcggctgtc gacatgttgc gcgtcaggcc cgcctggccg
      961 gtggcgcaga aggggcagtt catgccgcag cctgcctgcg acgacacgca cagcgtgatg
     1021 cggccgggat agcgcatcag caccgactcg accagggcgc cgtcgtgcag tcgccagagg
     1081 aacttgatgg tgtcgccgcg gtcggtggtg agccggcgca cctcggtcat gagcggcggc
     1141 agcatcccgc cgacgagttc ctcgcgctgc tccgcgggaa ggtcggtcat gtgcgccgga
     1201 tcggaggtgt agtgcgtgaa gtagtgggtc gacagctgct tcgcgcggaa gccggggagc
     1261 cccagctcct tcaccttcgc gatgcgctcc tcgggcgtca tgtcggcgag gtgcacgggc
     1321 ggcttgccgc gcttggggct cgcgaactgc agcagcggcc gcccctcggc atccttcttc
     1381 tgcgtccacc cctccgtcgc agggcgcacc tgcgtgcgag cgggcccggt ggtgcgcacg
     1441 ggcgtgctgc gggcaggcgg ggtcttgtcg gatgcaggca tgcatcaagg gtacggggaa
     1501 cgacccgcga gagggctgga tgcccggtat ccgagcctcc gggctggcag gctggtcggc
     1561 atggaaccgt ggatgtggat cgtggccgtc gtcgtcctgc tggtgctcgt gccgtgggcc
     1621 acgaagggga tgcggccgaa ggcgccgcag ccgacggcgt ccgtcgcacc tcgtgcactg
     1681 tctcccgcgg agtccgccga gatcgacgcc ctggtcgccg gcgaccggaa gatcgcggcg
     1741 atcaagcgcc tgcgggagct gcaatcgctg ggactgcagg aggcgaagga cgcgatcgac
     1801 gcctgggttc cggaaacgac gtggacgccg gccgcggcgg catccgcttc cgcgccgggg
     1861 gacgccctga cgcctgcagc acgcgccgag atcaccgcgc tcatcgccga cgacgagaag
     1921 atccgggcga tcaagcgcta tcgcgaggag accggcgtgg gtctgaagca ggcgaaggac
     1981 gccgtcgaga actggtgatc cgacttgtaa ttacaagtgc tttccgagca caatggaggc
     2041 gacgaacgga gccctcatga ccgaccgcct ctcccgtgcc cgcaccgccg gcgtcctcgc
     2101 cgtgctgcgc gctccctccc ccgagtccgc gctggaggcc gccgacgcga tcatccgcgg
     2161 cggcatcccc ggtatcgagg tcacgtacac cacacccgac gcccccgccg tgatccgcga
     2221 gctgatcgcc cgccatggcg acgatcagct tgccggtgtt gacgttgtgg atgcgcacct
     2281 cggtccgcgg atcctgcgcc tgcaccagcc cacgctcgat ggcatacggg cccacggctg
     2341 ccagca
//
LOCUS       DATMSX010000298         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_32998,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000298 DATMSX010000000
VERSION     DATMSX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32998"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN041_09080"
     CDS             <1..263
                     /locus_tag="VN041_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator PtsJ"
                     /protein_id="HWU29232.1"
                     /translation="GAFVSALAERGIETASSDGLSLWVRLAVPARTAAERLMRRGWLV
                     RTGDEFRIDEASPASDHLRLTVHDLSAADTARLLDDLEAAAR"
     gene            295..1146
                     /gene="pdxY"
                     /locus_tag="VN041_09085"
     CDS             295..1146
                     /gene="pdxY"
                     /locus_tag="VN041_09085"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HWU29233.1"
                     /translation="MKILSIQSAVAHGHVGNSAAVFPLQRIGVEVLPVYTVNFSNHTG
                     YGAWRGPMIAPDDVREVITGIEERGVLPQIDAVLSGYQGGEGIADVIIEAVAKVKAAN
                     PDAVYACDPVMGNAKSGCFVAPAIPELLRDKVVPVADIITPNQFELGYLTRTEPGDLE
                     STLASVDAAMAMGPSVVLVTSVERPDREEGTIEMLAVDGKGAWLVATPQLPMKANGSG
                     DVTAALFTAHYVETGDAKVALERTVSSVFDLLQNTLDSGERELQLVESQEAYANPRLQ
                     FTAYQVR"
     gene            complement(1152..1688)
                     /locus_tag="VN041_09090"
     CDS             complement(1152..1688)
                     /locus_tag="VN041_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29234.1"
                     /translation="MTGCSQATSPESKPMQKATPSTSASPVPERTAGVDAGDPQMADA
                     FAERDAFFAEQGFTGNGPLRATTEAQRALVQAQKEWTEAKGGVWDDRGETILLALGSD
                     TCETGILNQHRVDGTTLKKLIVSSPLTAQLIPKDTPADQAALYERNIASMSVFSASYL
                     CPADKDAWEAAFRTAYPG"
     gene            complement(1824..2384)
                     /locus_tag="VN041_09095"
     CDS             complement(1824..2384)
                     /locus_tag="VN041_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWU29235.1"
                     /translation="MTSTPEHEVAVEDGGHLAGVPDEFQRLWTPHRMAYIQAGPEVLR
                     EDCPFCEAPKHPDPERLIVARGETAYVLLNLFPYNSGHLLVCPYRHIATYDQATPEEV
                     AEIGALTQTGMRVLREVSNCDGFNLGMNQGAVAGAGVDAHLHQHIVPRWTSDANFFPI
                     IAKTKALPQLLGEVREAVAHAWPVDN"
     gene            complement(2381..4318)
                     /gene="thrS"
                     /locus_tag="VN041_09100"
     CDS             complement(2381..4318)
                     /gene="thrS"
                     /locus_tag="VN041_09100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWU29236.1"
                     /translation="MRVNGVLKDLAATVTENDVVEPVTIDSPAGLDILRHSTAHVLAQ
                     AVQRIRPAANLGIGPFITDGFYYDFGVDEPFTPEDLKAISKEMQRIVRDGQRFTRRVV
                     TEEEARAELANEPFKLELIDLAGGPGSGADAADGASVEVGAGELTIYDNVTKDGEVAW
                     KDLCRGPHVPSTRMLGNGWDLTRVAAAYWRGSEKNPQLQRIYGTAWPSKEELRAYQER
                     LAEAERRDHRKLGVEMDLFSFPDEIGSGLAVFHPKGGIIRYEIEENLRRHLLRNGYDV
                     VNSPHITKKDLFMTSGHLQTYADGMFPPMHLDEVVDDEGNVTRQGQDYYLKPMNCPFH
                     NLIYRARGRSYRELPLRLAEFGTVYRYEKSGTLSGLTRVRGLTQDDAHIYVAQDQVRE
                     ELTTNLNLVLELLRDYGLNDFYLELSTNEEGNPKFLGEPEQWTTAIDTLREVALESGL
                     ELVADPGGAAFYGPKISVQARDAIGRTWQMSTIQLDFNQPERFELEYTGPDGQKHRPV
                     MIHRALLGSVERFFAILLEHYAGDFPLWLAPMQVVGVPVADAFGDYLDEVVGRLKAEG
                     VRAEVDRSDDRMQKKIRNHTTAKVPVILIAGEQDRAAGTVSFRFRDGSQENGVPIADA
                     VARIRGAIASHARVMTSEDLA"
     gene            4651..4833
                     /locus_tag="VN041_09105"
     CDS             4651..4833
                     /locus_tag="VN041_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29237.1"
                     /translation="MNFADQTLLMLILNVSIFALATVIGLLLLYLVIRQGVFHGLRAH
                     TRWIDSGKDYKTVSQR"
     gene            complement(4906..4977)
                     /locus_tag="VN041_09110"
     tRNA            complement(4906..4977)
                     /locus_tag="VN041_09110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4943..4945),aa:Val,seq:gac)
     gene            complement(5005..5075)
                     /locus_tag="VN041_09115"
     tRNA            complement(5005..5075)
                     /locus_tag="VN041_09115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5041..5043),aa:Cys,seq:gca)
     gene            complement(5130..5202)
                     /locus_tag="VN041_09120"
     tRNA            complement(5130..5202)
                     /locus_tag="VN041_09120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5167..5169),aa:Gly,seq:gcc)
     gene            5418..5490
                     /locus_tag="VN041_09125"
     tRNA            5418..5490
                     /locus_tag="VN041_09125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5451..5453,aa:Val,seq:cac)
     gene            5492..5567
                     /locus_tag="VN041_09130"
     tRNA            5492..5567
                     /locus_tag="VN041_09130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5525..5527,aa:Gly,seq:gcc)
     gene            5628..>6062
                     /locus_tag="VN041_09135"
     CDS             5628..>6062
                     /locus_tag="VN041_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWU29238.1"
                     /translation="MTAGHVVIAGASGLIGRALTASLRADGIRVVHLVRRAPTGPDEV
                     RWLDSAHPLSPDVLAGADAVVGLNGASIGRLPWTRGYRDRLRSSRLLPTQTLAATLRA
                     LGGDAPAFLSASGIGFYGDRPGEALDESSGAGRTFLAELCVDW"
BASE COUNT          933 a         2033 c         2048 g         1048 t
ORIGIN      
        1 acggcgcgtt cgtctccgcc ctcgccgagc gcggcatcga gaccgcttcc tctgacgggc
       61 tcagcctctg ggtgcgcctc gcggtacccg cacggacggc ggccgagcgc ctgatgcgcc
      121 gcggctggct ggtgcgcacg ggcgacgagt tccgcatcga cgaggcatcc ccggcatccg
      181 accatctgcg cctgacggtg cacgatctca gtgccgcaga caccgcccgg ctgctcgacg
      241 atctcgaggc cgccgcgcgc tgaggcgcct cgcggcgggt gagaggatgg caggatgaag
      301 atcctctcga tccagtcggc cgtcgcgcac ggccacgtcg gcaactcggc cgccgtcttc
      361 ccgctgcagc gcatcggcgt cgaggtgctg ccggtctaca cggtcaactt ctccaaccac
      421 accggctatg gcgcctggcg cggaccgatg atcgcgccgg acgacgtgcg cgaggtcatc
      481 accggcatcg aggagcgcgg cgtgctgccg cagatcgacg ccgttctcag cggctaccag
      541 ggtggcgagg gcatcgccga cgtcatcatc gaggcggtcg ccaaggtcaa ggcggcgaac
      601 ccggatgccg tctacgcctg cgacccggtg atgggcaacg cgaagtccgg atgcttcgtc
      661 gcaccggcca ttccggagct tctgcgcgac aaggtggtgc cggtcgccga catcatcacg
      721 cccaaccagt tcgaactggg ctacctgacc cgcaccgagc cgggcgacct ggagtccacg
      781 ctggcgtccg tggacgccgc gatggcgatg ggcccctccg tcgtgctggt gacctcggtc
      841 gagcggcccg accgcgagga gggcaccatc gagatgctcg cggtggacgg gaagggcgcc
      901 tggctggtcg cgacccccca gctgccgatg aaggccaacg gctcgggcga tgtcaccgcg
      961 gcgctcttca ccgcgcacta cgtcgagacc ggcgacgcga aggtcgcact ggagcgcacc
     1021 gtgtcgagcg tcttcgacct gctgcagaac acactcgact cgggtgagcg cgagctgcag
     1081 ctcgtcgagt ctcaggaggc gtacgccaac ccgcgcctgc agttcaccgc gtaccaggtg
     1141 cgctgacgga atcagcccgg gtaggccgtc cggaaggcgg cctcccaggc gtccttgtcc
     1201 gcaggacaga ggtacgaggc gctgaacacc gacatcgagg cgatgttgcg ctcatacagc
     1261 gcggcctggt cagccggggt gtccttcggg atcaactgag ccgtcaatgg ggatgagacg
     1321 atcagcttct tcaacgtggt tccgtcgact ctgtgctgat tcaggatgcc ggtctcgcag
     1381 gtgtccgacc ccagagccaa cagaatcgtc tcgccccgat cgtcccagac tccgcctttg
     1441 gcctcggtcc actccttctg ggcctgcacc agtgcccgct gggcctcggt cgtggccctg
     1501 agcggcccgt ttcccgtgaa gccctgctcc gcgaagaacg cgtcgcgctc ggcgaaggca
     1561 tccgccatct gcgggtcacc ggcgtcgacc ccggccgtgc gctcgggcac tggtgaagcg
     1621 gacgtcgaag gcgtggcctt ctgcatcggc ttcgactccg ggctcgtcgc ctgcgagcag
     1681 cctgtcatga cgatgagagc aaccgcgctg caggcgacga ctgccgccac tcgcgaaaca
     1741 ggcatagaac tgcgaaccgc accgttcatc tttcccccct gagaaactgt gcgttcagtc
     1801 tcgcagaggc ggcgttccga aaatcagttg tccacaggcc aggcgtgcgc gaccgcttcg
     1861 cggacctcgc cgagcagctg cggcagtgcc ttcgtcttcg cgatgatcgg gaagaagttc
     1921 gcgtccgacg tccaccgcgg aacgatgtgc tggtgcaggt gcgcgtccac tcccgcgccg
     1981 gcgacggcgc cctggttcat gcccaggttg aagccgtcgc agttcgacac ctctcgcagc
     2041 acgcgcatgc cggtctgcgt cagcgcgccg atctccgcga cctcttccgg cgtcgcctgg
     2101 tcgtaggtcg cgatgtggcg gtagggacag accagcaggt gcccggagtt gtacgggaac
     2161 aggttgagca gcacgtaggc cgtctcgccg cgggccacga tgagccgctc gggatccgga
     2221 tgcttgggcg cctcgcagaa cgggcagtcc tcgcgcagca cctcggggcc tgcctggatg
     2281 taggccatgc ggtgcggagt ccacagccgc tggaactcgt ccggcacacc ggcgaggtgg
     2341 ccgccgtcct cgacggccac ctcgtgctcg ggcgtggagg tcacgcgaga tcctcggagg
     2401 tcatcacccg cgcatgcgac gcgatcgctc cgcggatgcg ggcgacggca tccgcgatcg
     2461 gcacgccgtt ctcctgcgag ccgtcgcgga agcggaacga cacggtgccg gcggcgcggt
     2521 cctgctcacc ggcgatgagg atgaccggca ccttggccgt ggtgtggttg cggatcttct
     2581 tctgcatccg atcgtcggag cggtcgacct cggcgcgcac gccttcggcc ttgagccgtc
     2641 ccacgacctc gtccaggtag tcaccgaacg cgtcggcgac cgggacgccc acgacctgca
     2701 tcggtgcgag ccacagcggg aagtcgcccg cgtagtgctc gagcaggatc gcgaagaacc
     2761 gctccaccga tccgagcagc gcgcggtgga tcatcaccgg gcggtgcttc tgcccgtcgg
     2821 gaccggtgta ctccagctcg aagcgctccg gctggttgaa gtccagctgg atggtcgaca
     2881 tctgccaggt acggccgatg gcgtcccgcg cctgcaccga gatcttcggg ccgtagaagg
     2941 ctgcgccgcc cgggtctgcg accagttcga ggccggattc cagggcgacc tcgcgcagcg
     3001 tgtcgatggc ggtcgtccac tgctcgggct cgccgaggaa cttcgggttg ccctcctcgt
     3061 tggtcgagag ctccaggtag aagtcgttca gcccgtagtc acgcagcagt tcgagcacca
     3121 ggttgaggtt ggtggtcagc tcctcgcgca cctggtcctg ggcgacgtag atgtgcgcgt
     3181 cgtcctgggt cagaccgcgc acgcgggtga ggcccgagag cgtgccgctc ttctcgtacc
     3241 ggtacacggt gccgaactcg gccaggcgca gcggcagctc gcggtagctg cgtccgcggg
     3301 cccggtagat caggttgtgg aacgggcagt tcatgggctt caggtagtag tcctggccct
     3361 gacgggtgac gttgccctcg tcgtcgacca cctcgtcgag gtgcatcggc gggaacatgc
     3421 cgtcggcgta ggtctgcaga tgacccgagg tcatgaacag atccttcttg gtgatgtggg
     3481 ggctgttcac cacgtcgtag ccgttgcgca gcagatggcg ccgcaggttc tcctcgatct
     3541 cgtagcggat gatcccgccc ttggggtgga agaccgccag tcctgagccg atctcgtcgg
     3601 ggaacgagaa caggtccatc tcgacgccga gcttgcggtg gtcgcggcgc tcggcctcgg
     3661 cgaggcgctc ctggtaggcg cgcagctctt ccttgctggg ccacgcggtg ccatagatgc
     3721 gctgcagctg cgggttcttc tcgctgcccc gccagtaggc tgcggcgacc cgcgtgaggt
     3781 cccagccgtt gccgagcatg cgggtgctgg gcacgtgcgg accgcggcag aggtccttcc
     3841 aggccacctc gccgtccttg gtgacgttgt cgtagatggt cagctcgccg gctccgacct
     3901 cgacggatgc cccgtcggcg gcgtccgcgc cggagccggg gccgccggcc aggtcgatca
     3961 gttcgagctt gaagggctcg ttcgcgagct cggcgcgcgc ctcctcctcg gtgacgacgc
     4021 ggcgggtgaa gcgctggccg tcgcggacga tgcgctgcat ctccttcgag atggccttga
     4081 ggtcctcggg cgtgaagggc tcgtccacgc cgaagtcgta gtagaagccg tcggtgatga
     4141 acgggccgat gcccaggttc gcggcaggac ggatgcgctg cacggcctgt gcgagaacgt
     4201 gcgcggtgga gtggcgcagg atgtcgaggc ctgcggggct gtcgatggtg accggttcga
     4261 cgacgtcgtt ctcggtgacg gtcgcggcga ggtccttgag gacgccgttc acccgcatgg
     4321 cgatgacgga tcgatcggtg aagagtgcga agccgtcgcg cccctcggcg ctgggctggg
     4381 cgatgtctga cacggggatt ctcctgatgg acgaatggaa tgagacgact cagcttcggg
     4441 cgcgacggcg ccctgcccgc tcaggcgcca gtagttcgcg tggcgacacc ggtgacagag
     4501 gtcacgagaa gggtcgacac ggattccatg ggaagagtct acgctgtcgg cccgaagccc
     4561 tcggaaacga cgaaaccccc gggggcaccg ggctcttctc cctgatcggg taggagagcg
     4621 gcttcgccgt gtgtcgctat ggtcgcgagt atgaacttcg ctgatcagac cctgctgatg
     4681 ctcatcctca acgtgtcgat cttcgctctc gcgaccgtga tcggccttct gctgttgtac
     4741 ctcgtcatcc ggcagggtgt cttccacggc ctccgtgcgc acacccgctg gatcgactcc
     4801 ggcaaggact acaagaccgt cagccagcga tgatgccaag catctgtttc ctccgagagg
     4861 tccccgatac aagaaaaccc ccggtaaacc gggggtttct gtctgtgcgc gatactggga
     4921 tcgaaccagt gacctcttcc gtgtcaggga agcgcgctac cgctgcgcca atcgcgccta
     4981 gaaggctatt aagttttcgc tgagaggtgg cgacgggatt cgaacccgtg taaacggctt
     5041 tgcaggccgg tgcctagccg ctcggccacg ccaccagagg tggattcgaa cccacatgcc
     5101 agaacccctg gctggggctc ctgcactcga gcggatgacg agactcgaac tcgcgaccct
     5161 caccttggca aggtgatgcg ctaccaactg cgctacatcc gcaaactgtt cagtttttcg
     5221 ttcccgcccg gcgtaccggg cactcgaatt acattagccc aggttcaggc gttcgccaaa
     5281 ccggatgacg ccgtcgggcg tgtccaccca caccgctcct gggcacgcca cgcccggcct
     5341 gccgaccgat accggtgctg cccatccgat cgggtatgct tttcaatcgt gccgttgtgc
     5401 acaccacaac agcacagggg cgattggcgc agttggtagc gcgcttcctt cacacggaag
     5461 aggtcgtcgg ttcgagtccg gcatcgccca cgcggatgta gcgcagttgg tagcgcataa
     5521 ccttgccaag gttagggtcg cgagttcgag tctcgtcatc cgctccacgt cagaggcccg
     5581 ggatcgatga tcccgggcct ctcgtcgtat ccggagggag acaggacatg accgcaggcc
     5641 acgtcgtcat cgccggcgca tccggcctga tcggacgcgc gctcacggcc tcactccgcg
     5701 cggacgggat cagggtcgtg cacctcgttc gccgcgcacc gaccgggccc gacgaggtgc
     5761 gatggctcga ttcagcgcat ccgctctccc ccgacgtcct cgccggtgcc gacgcggtgg
     5821 tcggcctgaa cggcgcgagc atcggcagac tgccgtggac gcgtggttac cgcgaccggc
     5881 ttcgatcctc tcggctgctg cccacgcaga cgctcgcggc caccctccgg gcactgggcg
     5941 gcgatgcacc ggccttcctc tcggcgtccg gcatcggctt ctacggcgac cgcccgggcg
     6001 aggctctcga cgagagcagc ggcgcgggcc gcacattcct cgcggagctc tgcgtcgact
     6061 gg
//
LOCUS       DATMSX010000299         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_137117,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000299 DATMSX010000000
VERSION     DATMSX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137117"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1632)
                     /locus_tag="VN041_09140"
     CDS             complement(<1..>1632)
                     /locus_tag="VN041_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWU29239.1"
                     /translation="GLTAAGMLLTGGIAPALADSGPSDAQGRTGQFDTPFAGFMKGST
                     MLRHSTPEALGLDPAPIEKAWAAIDGFAAPTAPGAKPMYASAVGVMGHQGRIVSEHHT
                     GDSLLYADGTTKLPEAQRIAAQQDTIYDMASVSKLFTSIVIMQLVEDGLVDLDATYGS
                     YVPEFANGGKETITIRQMLTHTSGLVSWLPLWSAYPDKDARIQAVMDVPVKNPPGTVY
                     EYSDLNLISLGVLAERLTGKSLDVLVAEGITAPLKMVDTGYNPDASEKPRIAATEYQT
                     SPARGMVWGSVHDENAWSLGGVAGHAGIFSTAADMAILAQTMLNGGTYGGERILSEAS
                     VRAMITDENTAFPGDSHGLGFELNQLWYMGGLGAGSTAGHTGYTGTSLVIDFTSRSFV
                     VLLTNRVHPSRSWGSNNPSRRAIAQGLAEALAVSPRKGRTEWFSGTEDASEHTLSVDV
                     AADSGVSLSFDVFADLESSDVFALEASPDGGSTWSLVPYTVRFSDRVVETDGTFDNQG
                     HRDWGRATATVPDGTDTLRWRLTTDANTQGRGAFVD"
BASE COUNT          243 a          564 c          561 g          264 t
ORIGIN      
        1 gtcgacgaac gcgccgcggc cctgcgtgtt ggcatccgtg gtgagccgcc agcgcagcgt
       61 gtcggtgccg tccggcacgg tggccgtggc cctgccccaa tcccggtggc cctggttgtc
      121 gaacgtgccg tcggtctcga cgacgcgatc gctgaagcgc accgtgtacg gcacgagcga
      181 ccacgtcgac ccgccgtcgg gcgacgcctc gagggcgaac acgtcgctcg actccagatc
      241 ggcgaacacg tcgaacgaca gcgacactcc gctgtcggcg gccacgtcga ccgagagcgt
      301 gtgctcggag gcatcctcgg tgccactgaa ccactccgtc ctgcccttgc ggggcgagac
      361 ggcgagggcc tcggcgaggc cctgcgcgat cgcacgacgc gacggattgt tcgaacccca
      421 gctgcgactc gggtgcaccc ggttggtcag cagcacgacg aacgaccgcg acgtgaagtc
      481 gatcaccagc gacgttccgg tgtacccggt gtgcccggcg gtggagccgg cgccgagccc
      541 gcccatgtac cagagctggt tcagctcgaa gccgagaccg tgggagtcgc cggggaaggc
      601 ggtgttctca tcagtgatca tcgcccgcac cgacgcctcg gacaggatcc gctcgccccc
      661 ataggtaccg ccgttgagca tggtctgcgc gaggatcgcc atgtcggccg cggtggagaa
      721 gatgccggcg tggcctgcca cgccgcccag gctccacgcg ttctcgtcat gcactgagcc
      781 ccagaccatg ccccgggcgg gggaggtctg gtactcggtc gcagcgatcc ggggcttctc
      841 gctcgcgtcc gggttgtagc cggtgtcgac catcttcagc ggcgcggtga tgccctccgc
      901 gaccagcacg tcgagagact tgccggtcag ccgctcggcg agcacgccca gcgagatgag
      961 gttgagatcg gagtactcgt acacggtgcc cggcgggttc ttcaccggca cgtccatcac
     1021 cgcctggatg cgcgcatcct tgtcggggta cgcgctccac agcggcagcc acgatacgag
     1081 acccgatgtg tgcgtgagca tctgccggat cgtgatcgtc tccttgccgc cgttcgcgaa
     1141 ctccggcacg tagctgccgt aggtcgcatc cagatcgacg aggccgtcct ccaccagctg
     1201 catgatcacg atcgaggtga agagtttgct caccgacgcc atgtcgtaga tggtgtcctg
     1261 ctgcgcggcg atgcgctgcg cctcgggcag cttggtcgtg ccgtccgcgt acagcaggga
     1321 gtcgccggtg tgatgctcgc tgacgatcct gccctggtgg cccatcacgc cgaccgccga
     1381 cgcgtacatc ggcttggcgc ccggagcagt cggggcggcg aagccgtcga tcgccgccca
     1441 ggccttctcg atcggtgcgg gatccagccc gagcgcctca ggggtgctgt gccgcagcat
     1501 cgtggagcct ttcatgaaac ccgcgaaggg ggtgtcgaac tgaccggtcc tgccctgggc
     1561 gtcggaaggc ccgctgtccg cgagcgccgg cgcgatgccg ccggtgagca gcatccccgc
     1621 ggcggtcagc cc
//
LOCUS       DATMSX010000300         4557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_165520,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000300 DATMSX010000000
VERSION     DATMSX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165520"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VN041_09145"
     CDS             <1..1014
                     /locus_tag="VN041_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29240.1"
                     /translation="PGYAQPGYAQPQPGQPQAPQQPPYVGYAQQPAYSPQGYPVTSYP
                     GYGAAPGQLPNPGVGGLAIAAIIVSGIAFLSGWIPIVGVVLALVGLVLSILAIRSGRG
                     KVLGIIGTILAGIALLFGAFMTTMIFVVAPWNDMSSSSSDPWSVETPSAAPDDGAFDE
                     AEAATVSGQFIETPCWSYDGPKHFTDNISAEEAGNCYGQLELWGEYDESGDFVPSGYG
                     ETAGQITVEPVSSATAAQYSDGTDLSATVDALEKPYLSTVGGTISSLHEETTLGGVPA
                     NITRIDSDVAETQMKVVLTTFSPQPYDIGGDQRKLFLISITTPYSNGEEQLQQLLDSW
                     EWR"
     gene            1053..2507
                     /locus_tag="VN041_09150"
     CDS             1053..2507
                     /locus_tag="VN041_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29241.1"
                     /translation="MKAILVTTGRTLARHWPAMLAWYLGGIAVHYVIVQVAGIVGAHS
                     ATVGFLILPVAILARLISYVAMFLVLRDGLRELDAIAPLPESAADRRKTFLTALLAGI
                     LPFLAVYWGQGLLTDDVVAYSARALRERTDIIFQAALTPGQRLSTDDTVLNLPLNVWT
                     IAIIVVAFAARWAWTKWQQRIPGWVSLIAVYLEVVWVFFSVIVIGDITDRVKAWVDTR
                     AAMAWLEQVRENVLDAVAPLRWAWDGIGWLLGEAGPILLAPLAWLTVGGVVYGQAIVA
                     EKLRIENELLANLREHAAVVPNPIMRRLKDLGDELGSRFVPIGRALLLMWRAGPVLIA
                     SYALLHVAVKTLETYLQYGAVRLVGPHELVFWGIALPLVSLVPVLLTEPLRVAVIAGA
                     YDATLGRLRQRQQKKVDAGVVEQAEADVRAVADAAGVLPDAVLEAGLKPETTGGQGSM
                     ENLTNRPSPRGSTSSQNGPVTSSGTTNPTVSENG"
     gene            complement(2390..2998)
                     /locus_tag="VN041_09155"
     CDS             complement(2390..2998)
                     /locus_tag="VN041_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29242.1"
                     /translation="MTIPTPAVAEAPEPQTPSPRQPGWWRTNRLALIALLVLIPAIGI
                     GVGWNEWYSYYGYGARKVTAVEVPDKGTVKVAGAEWGPIRSGEIKDTSTLNMPEGTRL
                     LAAIVPVTPDGKAVSCEPPMLVQQSTGREWTPMRSEVGIPYSYDENDTCVSDLADEKD
                     PESAQPFSLTVGFVVPDDVTGPFWLDVDPRGDGRFVRFSIDP"
     gene            complement(2995..3543)
                     /locus_tag="VN041_09160"
     CDS             complement(2995..3543)
                     /locus_tag="VN041_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29243.1"
                     /translation="MKKVLPWLLTLVLLAGAWVLNAVALPDGSSEASFLTPATVGHAA
                     TARNLTLTVTNVRAARSIAGPTGKTLESTWVVVDVDAEALDSQYGATLGFAELTVGER
                     TFRATERIESFFRARLVPGVPRSGAVAFELPSDALKGTGTLRLGIPSDARYDGLVELR
                     VDLGDIPVEDAITLPATDWTRS"
     gene            complement(3540..4208)
                     /locus_tag="VN041_09165"
     CDS             complement(3540..4208)
                     /locus_tag="VN041_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29244.1"
                     /translation="MNAAPPSPTQERTLLTRVRRMASLIPTPWLITAGGAVLLAATAA
                     FGGLQTVPPKPVPVVSVGEQYSGSDLEMTVQGVELRAKGGNAASFPDKAKHEKVLAVI
                     VDVVNTFDKPRLASGTGTPSPAVDGIHVDGIDEKPLIAYADGSSGSVWLQPDVPVRLV
                     LSWVVGPDELHDGDEVRLTLPDSTHSVGTNVQRGVDIWDEVVVGATVTTTVQEIPASD
                     EDAS"
     gene            4288..>4557
                     /locus_tag="VN041_09170"
     CDS             4288..>4557
                     /locus_tag="VN041_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29245.1"
                     /translation="MSEQPSVAPGARLEDAAVELTRRWVQEEASIPVDPAAQRLAGVL
                     RDPNGLPFTLGFVDGVMRPESAAAAASNLHRVAPLAPGFLPWYLRG"
BASE COUNT          659 a         1589 c         1586 g          723 t
ORIGIN      
        1 cccggctacg cgcagcccgg ctacgcgcag ccccagcccg gtcagccgca ggcaccgcag
       61 cagccgccct acgtcggcta cgcgcagcag cccgcctaca gcccgcaggg ctatcccgtg
      121 acctcctatc cgggatacgg cgcggccccc gggcagctcc cgaaccccgg cgtcggcgga
      181 ctcgcgatcg ccgcgatcat cgtctcgggc atcgccttcc tcagcggctg gatcccgatc
      241 gtcggagtcg tcctcgcgct cgtcgggctc gtgctcagca tcctcgcgat ccgcagcggc
      301 aggggcaagg tgctcggcat catcggcacc atccttgccg gcatcgcgct gctgttcggc
      361 gcgttcatga cgaccatgat cttcgtcgtc gcgccgtgga acgacatgtc gagcagctcc
      421 tccgatccct ggtccgtcga gacgccctcg gccgcccccg acgacggcgc cttcgacgag
      481 gccgaggccg cgacagtgtc cggccagttc atcgagacgc cgtgctggag ctacgacggt
      541 ccgaagcact tcaccgacaa catctcggcg gaagaagcag gaaactgcta cggccagctc
      601 gagctgtggg gcgagtacga cgagagcggc gacttcgtcc ccagcggcta cggcgagacc
      661 gccgggcaga tcacggtgga gccggtcagc tcggccacgg ccgcccagta ctcggacggc
      721 accgacctga gcgcgacggt ggacgccctg gagaagccct atctgagcac ggtcggcggc
      781 acgatctcga gcctgcatga ggagaccacg ctgggcggcg tgccggcgaa catcacgcgc
      841 atcgacagcg acgtggccga gacgcagatg aaggtggtcc tgaccacgtt ctcgccccag
      901 ccgtacgaca tcgggggcga ccagcgcaag ctgttcctga tcagcatcac cactccgtat
      961 tcgaacggcg aggagcagct gcagcagctc ctggacagct gggagtggcg ctgagcacgc
     1021 gcccgctcca cgactcgcga tagcgtgatg ccgtgaaggc gatcctcgtc acgaccgggc
     1081 gcacgctggc ccggcactgg cccgcgatgc tcgcgtggta cctcggcggt atcgccgtgc
     1141 actacgtcat cgtgcaggtc gccggcatcg tcggcgcgca ctccgccacg gtggggttcc
     1201 tcatccttcc ggtcgcgatc ctcgcgcggc tgatcagcta tgtcgcgatg ttcctggtgc
     1261 tgcgggatgg actgcgcgag ctcgacgcga tcgcgccgct gccggagtcg gccgccgatc
     1321 ggcgcaagac attcctcacg gcgctgctcg cgggcatcct gcccttcctc gccgtgtact
     1381 gggggcaggg actgctgacg gatgatgtgg tggcgtactc cgcgcgcgcc ctgcgcgagc
     1441 gcacggacat catcttccag gctgcgctga ccccgggtca gagactgagc accgacgaca
     1501 cggtgctgaa cctgccgctg aacgtgtgga cgatcgcgat catcgtggtc gccttcgccg
     1561 cccgctgggc gtggacgaag tggcagcagc gcatccccgg ctgggtctcg ctgatcgcgg
     1621 tgtacctcga agtggtctgg gtgttcttct cggtgatcgt gatcggcgac atcaccgacc
     1681 gcgtgaaggc ctgggtggac acccgtgccg cgatggcgtg gctggaacag gtgcgcgaga
     1741 acgtgctgga tgccgtcgcc ccgctgcgct gggcctggga tggcatcggc tggctgctcg
     1801 gcgaggcagg gcccatcctg ctggccccgc tggcctggct cacggtcggc ggtgtggtct
     1861 acgggcaggc gatcgttgcc gagaagctgc gcatagagaa cgagctgctg gcgaacctgc
     1921 gcgagcacgc cgccgtggtg cccaacccga tcatgcgacg gctgaaggat ctcggtgacg
     1981 agctcggctc ccgcttcgtg ccgatcgggc gcgcgctgct gctgatgtgg cgggcgggtc
     2041 ccgtgctgat cgcctcctac gcgctgctgc acgtcgcggt gaagaccctg gagacctacc
     2101 tgcagtacgg cgccgtgcgc ctggtcggac cgcacgaatt ggtgttctgg ggcatcgcgc
     2161 tcccgctggt gtctctggtg ccggttctgc tgacggagcc gctgcgcgtc gcggtgatcg
     2221 cgggggcgta cgacgcgacg ctggggcggc tgcgccagcg tcagcagaag aaggtcgatg
     2281 ccggcgtggt cgagcaggcc gaggccgacg tgcgtgccgt ggcggatgcc gccggcgtgc
     2341 tgccggatgc cgtgctcgag gccggcctga agcctgagac caccgggggt caggggtcga
     2401 tggagaacct gacgaaccgg ccgtcgccgc gcgggtcgac atcgagccag aacgggccgg
     2461 tgacatcgtc cggcacgacg aatcccacgg tcagcgagaa cggctgagcc gattccgggt
     2521 ccttctcgtc ggcgaggtcg ctgacgcagg tgtcgttctc gtcgtagctg tacgggatgc
     2581 cgacctcgga gcgcatcggc gtccattcgc gtccggtgct ctgctgcacg agcatcggcg
     2641 gttcgcagga gacggccttg ccgtcgggcg tcacaggcac gatcgcggcg agcaggcggg
     2701 tgccctccgg catgttcagc gtgctggtgt ccttgatctc gccgctgcgg atcggacccc
     2761 attcggcacc ggcgaccttc accgtcccct tgtccggaac ctcgaccgcg gtcaccttgc
     2821 gcgccccgta gccgtagtag gagtaccact cgttccagcc cacgccgatt ccgatggccg
     2881 ggatcagcac gagcagcgcg atcagcgcca ggcggttggt gcgccaccat ccgggctgcc
     2941 tcggtgaggg cgtctggggt tccggggcct ctgcgacggc gggcgtgggg atcgtcatga
     3001 gcgcgtccag tccgtggcgg gcagggtgat ggcatcctcc acgggaatgt cgccgaggtc
     3061 gaccctcagc tcgacgaggc cgtcgtagcg ggcatccgac gggatgccca gacgcagcgt
     3121 tcccgtcccc ttcagcgcat cggagggcag ctcgaaggcc accgcgccgc tgcgcggcac
     3181 accggggacg agccgcgcgc ggaagaagct ctcgatgcgc tcggtggcac ggaacgtgcg
     3241 ttcgccgacc gtcagctcag cgaatccgag cgtcgcgccg tactgggagt cgagtgcctc
     3301 ggcatccaca tcgaccacca cccaggtgct ctccagcgtc tttcccgtcg ggccggcgat
     3361 gctccgagcc gcgcgcacat tcgtcaccgt gagcgtgagg ttgcgcgcgg tcgcggcgtg
     3421 gccgaccgtg gcgggtgtga ggaagctcgc ctcggacgag ccgtccggca gggccacggc
     3481 gttcagtacc caggcgccgg cgagcagcac gagcgtcagc agccagggga gcaccttctt
     3541 catgaggcat cctcatcgct cgcggggatc tcctgcacgg tcgtggtcac agtggcgccg
     3601 accaccacct cgtcccagat gtccacgcca cgctgcacat tggtgcccac gctgtgcgtg
     3661 gaatcgggaa gggtcaggcg cacctcgtcg ccgtcgtgca gttcatccgg cccgacgacc
     3721 cacgacagga cgagccgcac cgggacgtcg ggctgcagcc agaccgaccc ggagctgccg
     3781 tcggcgtagg cgatcagcgg cttctcgtcg atgccgtcga cgtggatgcc gtcgacggcg
     3841 ggcgacggag tgccggtccc gctcgccaac cggggcttgt cgaaggtgtt caccacatcc
     3901 acgatcacgg cgagcacctt ctcgtgcttt gccttgtcgg ggaaggatgc cgcattgccg
     3961 cccttcgcgc ggagctccac cccctggacc gtcatctcca gatccgatcc ggagtactgc
     4021 tctccgacgg acacgacggg gaccggcttc ggcggcaccg tctgcaggcc gccgaacgcc
     4081 gccgtcgcag ccagcagcac cgcgccgccg gccgtgatca gccacggcgt cgggatcagg
     4141 ctcgccatgc gccgcacccg cgtgagcaga gtcctctcct gagtgggcga gggcggggcg
     4201 gcgttcacga gatcagcata gggtgacttc cgccggtgcc accgggttcc gccgccttcc
     4261 cgcgcggtcg ggacctacgc tgggaacatg tctgagcagc cgtccgtcgc acccggggcg
     4321 cgtctggagg acgccgccgt cgaactcacc cgtcgctggg tgcaggagga ggcgtcgatc
     4381 cccgtcgatc ccgcagcgca gcgcctggcc ggcgtgctgc gcgatccgaa cggcctgccg
     4441 ttcaccctgg ggttcgtcga cggcgtcatg cggccagaga gcgccgcggc cgcggcatcc
     4501 aacctgcacc gcgtcgcacc gctcgctccc gggttcctgc cctggtacct gcgtggc
//
LOCUS       DATMSX010000301         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_146639,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000301 DATMSX010000000
VERSION     DATMSX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146639"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="dusB"
                     /locus_tag="VN041_09175"
     CDS             complement(<1..739)
                     /gene="dusB"
                     /locus_tag="VN041_09175"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWU29246.1"
                     /translation="MTLATDTATTPALRIGPIDLDVPVVLAPMAGITNTAFRRLCREY
                     GAGLYVSEMITSRALVERNAVTMRLIQHHESETTRSIQLYGVDPATIAEAVRIIVAED
                     RADHVDLNFGCPVPKVTRKGGGAALPWKIGLFADIVDRAVKAAGDVPLTVKMRKGINK
                     DHLTFLEAGRAAEDAGAAAVALHARTASEYYSGHADWNAIGELKQAVTSIPVLGNGDI
                     WSADDAVRMMAETGADGVVVGRGCLGRP"
     gene            801..1304
                     /locus_tag="VN041_09180"
     CDS             801..1304
                     /locus_tag="VN041_09180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HWU29247.1"
                     /translation="MTETTLRQIPFSDAEGTTKTLDDYGSDVVLVVNVASKCGLTPQY
                     EQLEQLQREYGDRGFSVVGFPCNQFMGQEPGSMEQILEFCSTTYGVTFPVNGKVKVNG
                     RNAHELYKALKQTSDAGGKAGRVEWNFEKFLVSADGSVQRFRPKQKPDAPEIVSAIED
                     ALVAAGR"
BASE COUNT          226 a          517 c          566 g          253 t
ORIGIN      
        1 acggccggcc caggcagcct cggccgacga ccacgccgtc ggcgccggtc tccgccatca
       61 tccgcacggc gtcgtcggcc gaccagatgt cgccgttgcc gagcaccggg atgctggtga
      121 cggcctgctt gagctcgccg atcgcgttcc agtcggcatg gccggagtag tactcggatg
      181 cggtgcgggc gtgcagcgcg acggccgccg ctccggcgtc ctccgccgcg cgtcccgcct
      241 ccaggaaggt caggtggtcc ttgttgatgc ccttgcgcat cttgaccgtg aggggcacgt
      301 cgcctgcggc cttgacggcg cggtcgacga tgtcggcgaa caggccgatc ttccagggaa
      361 gcgccgctcc cccgcccttg cgggtgacct tgggcaccgg gcatccgaag ttgaggtcga
      421 catggtcggc gcggtcctcc gcgacgatga tgcggacggc ctcggcgatg gtggccgggt
      481 ccacgccgta gagctggatc gaacgggtcg tctcgctctc atggtgctgg atcaggcgca
      541 tcgtgacggc gttgcgctcc accagggcgc gggaggtgat catctcgctg acgtagaggc
      601 ctgcaccgta ctcgcggcag agccgccgga aggcggtgtt ggtgatgccc gccatcgggg
      661 cgagcacgac gggtacgtcg aggtcgatcg gtccgatgcg gagcgcgggg gtcgtggcgg
      721 tgtcggtggc gagagtcatg tcctctccat tctcccagac gtcggcgccc ggtgggcgcg
      781 gcggaattag ggtggggagt atgaccgaaa cgacgctgcg acagatcccg ttctccgacg
      841 cggagggcac cacgaagacg ctcgacgact acggctcgga cgtggtgctg gtggtcaacg
      901 tggcctccaa gtgcggcctg accccgcagt acgagcagct cgagcagctg cagcgcgagt
      961 acggcgaccg cggcttctcg gtggtgggct tcccctgcaa ccagttcatg ggccaggagc
     1021 cgggttcgat ggagcagatc ctggagttct gctccacgac gtacggcgtg acgttccccg
     1081 tgaacgggaa ggtcaaggtc aacggccgca acgcccacga gctgtacaag gcgctcaagc
     1141 agacgtcgga cgccggcggg aaggccgggc gcgtggagtg gaacttcgag aagttcctgg
     1201 tctccgccga cggctcggtg cagcggttcc gtcccaagca gaagccggat gccccggaga
     1261 tcgtgtccgc gatcgaggac gccctggtgg ccgcggggcg ctgagtccgg catccggcgt
     1321 ccggccgtcg ttcatgagcg cttcaggcgg tcatgcgcct cagccgggcg catcgttcac
     1381 cttcccgtcg gacgccggcc atctccggct cctaccgtct ccgcggcgat gcatcgcccg
     1441 agacgaagga gcccttgaat gtctgatcga ctgccgctga cccggcgcac cgtcctgacc
     1501 gcgggagcgc tcggcgccgt cagcgcggca ctgcccgctg tggccgcgag tgccggcacg
     1561 ac
//
LOCUS       DATMSX010000302         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_70853,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000302 DATMSX010000000
VERSION     DATMSX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70853"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VN041_09185"
     CDS             <1..760
                     /locus_tag="VN041_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29248.1"
                     /translation="QLAERLRRVPVADLLQVQEHLGAGAVPGPHSMRFSPVVGRDPLP
                     LVPTAAARSGIGAGIPLIIGTARDESHIALGADPGAASRDLPSEALHRLVADGLDRPE
                     DASRILRRYRELLPDSPPGELWLSIASDQFRIRSLRLAEARLGGGAAATYVYRYDVPD
                     AAGSVPHGAEVAAFFGRTADTAAADCLAAFARTGSPGPSWEAYARGSRHEHRFRETGA
                     ADVPDPDAERRLAWDGIGTGPRSHPWARLLEPAE"
     gene            802..1935
                     /locus_tag="VN041_09190"
     CDS             802..1935
                     /locus_tag="VN041_09190"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29249.1"
                     /translation="MTTRKQTAVQKACARGEAGIGALAASATDHVRAELPRYALVPVE
                     QHRGTVRDTIQSLLHALAGGGGFSADDAKAIRYAALRREGTGIPVQDILAAFHVVGRD
                     VWDALAHVEGISAGTLVQLADPLWSWIQRASALVAETSAAGGGAQGRQVVLRQRLLES
                     VRAGDAAEGDAVENARRLGFVPGGSFRAVCTEAAPWVEGDLELLQRGLARLPGTNHAG
                     LHGTLMIVLCQGASPQAVAEEVHRQLPGHGSVGVGLERPGVSGAETSIGDAERALRVA
                     WPESVSFFERAWLHSGLVDARTRLTPLYGEAKAIAAEHPGLAQAVRAFADEGFSLAAA
                     AAALQLHPNTVAYRLDRWHELTGHDPRRFDGLALSMLSIGPPR"
     gene            2123..3085
                     /locus_tag="VN041_09195"
     CDS             2123..3085
                     /locus_tag="VN041_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU29250.1"
                     /translation="MRLLSVGPAGAEEPFALIDDAGVDGPLSPEAVVVPLRPLAARHG
                     IRIEKLSDVLEQLADLARWLAEPQVRDEGERLGDRRIGAPIPVPRLVVGVGMNYPAHA
                     AGLDTRAGDPVLFLKPPTAVCGPYDDIVRPPETTALDHEVELGVVIGTAAHRVSAERA
                     LTHVAGFVVADDVSARDIALGAGLAHPLSLQLTRAKGFPTFCPLGPWLLTVDETPQPA
                     DLLLELSVNGAVRQRARAGEMITGIAELVAFVSAGIPLEPGDIILTGSPPGCGFEQDP
                     PVFLLPGDVVEAGVTGLGRMRTRVIDERDPSRREQPTINPRSAT"
     gene            3082..3552
                     /locus_tag="VN041_09200"
     CDS             3082..3552
                     /locus_tag="VN041_09200"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWU29251.1"
                     /translation="MIRTARPFIEAINPSRLAPPAGHFDRAMRIGPWLTVSGTSALTN
                     LTGPVEERRLPDDFRAQAELAFDNIAVVLDSVDASFEHVYEIHATLARAEDFGTLNDI
                     FRERIPERGFVGSGYVAEFLGPGMLIEVEVRAYLPHFADDTTTPHPITPNGAHA"
     gene            3549..>4271
                     /locus_tag="VN041_09205"
     CDS             3549..>4271
                     /locus_tag="VN041_09205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWU29252.1"
                     /translation="MTIPTLLGSRIVDLTTPLGPSTPVVPVPEPFAPTAALSFTPVSD
                     FDEHGPGWSWSDITLGEHAGTHVDAPRHWISGRDGRSVDQIPPVRLVGPVGVLDCTAE
                     VAENPGYLVTVADLEGWEAEHGRIQPGTWLFVRTGWSARGDDPARFLNDNVWPGLTLK
                     AAKWLAAHPNVSGLGVEAIGIDGLGAGPEADDAQDDPGAFGPGHYYLLGSDKYGLTSL
                     RNLDALPATGAWVVVAPLPLVGG"
BASE COUNT          608 a         1561 c         1495 g          607 t
ORIGIN      
        1 gcagctcgcg gaacggctcc gccgcgttcc ggtcgccgat ctcctgcagg tgcaggagca
       61 cctcggcgcg ggtgccgtcc ccggcccgca cagcatgcga ttctcgccgg tcgtcggacg
      121 agacccgctg cccctcgtgc cgaccgccgc cgcgaggtcc ggcatcggag caggcatccc
      181 cctcatcatc ggcacggcgc gcgacgagtc gcacatcgca ctcggagccg atccgggggc
      241 agcatcgcgt gacctgccat cggaagcact gcaccggctc gtcgcggatg ggctggacag
      301 gccggaggac gcatcgcgca tcctgcggcg gtaccgcgag ctgctgccgg actccccgcc
      361 gggggagctc tggctgtcga tcgcctccga ccagttccgg atccgcagcc tccgtctcgc
      421 cgaggcgcgt ctgggcggcg gcgccgccgc gacgtacgtg taccgctacg acgtgcccga
      481 cgcggcggga tcagtcccgc acggcgcgga ggtcgcggcg ttcttcggca ggactgcgga
      541 cacagccgcg gcggactgcc tcgcagcctt cgcccgcact ggttctcccg gcccgtcctg
      601 ggaggcatac gcgaggggct cgcggcacga gcaccggttc cgtgagacgg gggcggcgga
      661 cgttccggat ccggatgccg agcgtcgcct ggcctgggac ggcatcggca ccggcccccg
      721 gtcgcatccg tgggcgagac tgctcgaacc cgcggagtaa cctgcattcc acgacgagat
      781 cgcccttcgc ccgggagagg catgacaacg aggaagcaga ccgcggtgca gaaggcgtgc
      841 gccaggggcg aggcagggat cggcgcgctg gcggcctccg ccacagatca tgtgcgcgcc
      901 gagctccccc gatacgccct ggtgccggtg gaacagcacc gcggaaccgt gcgcgacacc
      961 atccagtcac tgctgcacgc gctcgcgggc gggggcggct tctcggccga cgatgcgaag
     1021 gcgatccgct acgcggcgct gcgccgggag ggcaccggca tcccggtcca ggacatcctc
     1081 gcggcgttcc atgtggtggg acgggacgtg tgggatgcgc tcgcgcatgt cgaggggatc
     1141 tcggccggca cgctggtgca gctggctgat ccgctgtggt catggatcca gcgggcgagc
     1201 gcgctggtcg ccgagacatc ggccgcaggc ggtggcgcgc aggggcgcca ggtcgtgctg
     1261 cgtcagcgcc ttctcgaatc cgtccgcgcc ggcgacgccg cggaaggcga cgcggtggag
     1321 aacgcccgtc ggctggggtt cgtccccggg ggcagcttcc gcgccgtgtg cacggaagca
     1381 gcgccctggg tggagggcga tctggagctg ctgcagaggg gcctcgcgcg cctgcccgga
     1441 acgaatcatg ccgggttgca cggaactctg atgatcgtgc tctgccaggg cgcgtccccg
     1501 caggcggtgg ccgaggaggt gcaccggcag ctgcccggcc acggcagcgt cggagtcggc
     1561 ttggagcggc ccggcgtatc gggggccgag accagcatcg gcgacgcgga acgggcgcta
     1621 cgggtcgcgt ggccggaatc cgtctcgttc ttcgagcgtg cgtggctgca ttcgggtctg
     1681 gtggatgcgc gtacccgcct cacgcccctc tacggggagg cgaaggcgat cgccgcggag
     1741 catccgggtc tcgcgcaggc cgtgcgggcc ttcgcggacg agggcttcag cctcgctgcg
     1801 gccgcggcgg cactgcagct gcatccgaac accgtcgcct accggctgga tcgctggcac
     1861 gagctcaccg gacacgaccc gcgacgcttc gacggcctgg cactctcgat gctgtcgatc
     1921 ggcccgccgc gctgaatccc ggagccgatc ggcgacccgc aatggcctga ccgccgacgc
     1981 acgcaactca ttcgtggaat ccacgaagaa ccagagccca agagcaccta tccgccgagg
     2041 aactcagcgt cctcgtcctc ctagtgtcat cgaaacgaca ggcacgagga ggagtcatgg
     2101 tcgacgaggt caggcatcgc gcatgcgtct gctgagcgtc gggcccgccg gcgcagagga
     2161 gccgttcgcg ctcatcgacg acgccggagt cgacggcccg ctttcgccgg aagcggtggt
     2221 cgtgccgctg cgcccgctcg ctgcacgcca cggcatccgg atcgagaaac tgagcgacgt
     2281 gctggagcag cttgcggacc tggcccgctg gctggcagaa ccgcaggtgc gcgatgaggg
     2341 ggagcgcctc ggtgatcgcc gcatcggcgc gcccatcccg gtcccgcgtc tcgtggtcgg
     2401 cgtcggcatg aactacccgg cgcacgcggc cgggctcgac acccgcgccg gggatcccgt
     2461 gctgttcctc aagccgccga ccgccgtgtg tgggccgtac gacgacatcg tccgcccgcc
     2521 cgagacgacc gcgctggacc acgaggtcga gctcggcgtc gtgatcggca cggccgccca
     2581 ccgcgtctcc gcggaacgcg ccctcacgca cgtggcgggg ttcgtggtgg cggacgatgt
     2641 ctcggctcgg gacatcgcgc tcggggcggg cctcgcgcac ccgctctctc tgcagctcac
     2701 ccgcgccaag gggttcccca ccttctgccc gctcgggccc tggctgctga cggtcgatga
     2761 gacaccgcag ccggccgacc tgcttctgga gctgagcgtg aacggcgcgg tgcgccagcg
     2821 agcacgagcc ggggagatga tcacgggcat cgcagaactc gtcgccttcg tcagcgcggg
     2881 aatcccgctc gagccgggcg acatcatcct caccggatcc ccgccggggt gcggattcga
     2941 gcaggatcct ccggtcttcc tgctccccgg cgacgtcgtc gaggccggag tcaccgggct
     3001 cggacgcatg cgcacgcgcg tcatcgacga gcgggaccca tccaggcgag aacagccgac
     3061 catcaacccg aggagcgcca catgatccgc actgcacgcc cgttcatcga ggcgatcaac
     3121 ccgtcccgtc tcgcgccgcc cgcggggcac ttcgaccgtg cgatgcgcat cggtccctgg
     3181 ctcaccgtct ccggcacatc cgcgctcacg aacctcaccg gccctgtcga ggagcggcgc
     3241 ctgcccgacg acttccgcgc gcaggcggag ctggccttcg acaacatcgc cgtcgtcctc
     3301 gactcggtcg acgcgagctt cgagcacgtc tacgagatcc acgcgaccct ggcgcgcgcc
     3361 gaggacttcg gcacgctgaa cgacatcttc cgcgagcgga tccccgagcg cggcttcgtg
     3421 ggcagcggct acgtcgccga gttcctcggc cccgggatgc tcatcgaggt cgaggtcagg
     3481 gcctatctcc cgcatttcgc cgacgacacc accacacctc acccgatcac cccgaacgga
     3541 gcacacgcat gaccattccc acccttctcg gatcgcgcat cgtcgatctg accacgccac
     3601 tgggcccgtc cacccccgtg gtccccgtgc cggagccctt cgccccgacg gcagcgctct
     3661 cgttcactcc ggtgagcgac ttcgacgagc acggccccgg ctggtcctgg agcgacatca
     3721 ccctcggcga gcacgccggc acccacgtcg acgcgccgcg gcactggatc agcggtcggg
     3781 acggccgctc ggtcgaccag atcccgccgg tgcgcctggt gggtccggtc ggcgtgctgg
     3841 actgcacggc cgaggtcgcc gagaaccctg gctacctcgt caccgtcgcc gacctggagg
     3901 gctgggaggc ggagcacggc cggatccagc cgggcacctg gctcttcgtg cgcaccggct
     3961 ggagcgcgcg cggtgacgat ccggcgcgat tcctgaacga caacgtctgg ccgggcctca
     4021 ccctgaaggc cgccaagtgg ctggcggcgc atccgaacgt ctccggcctc ggtgtcgagg
     4081 cgatcggcat cgacggtctc ggtgccggac cagaggcgga tgacgcgcag gacgatcctg
     4141 gggccttcgg tcccggccac tactacctgc tcggatcgga caagtacggc ctgacctcgc
     4201 tgcgcaacct cgacgccctc ccggcgaccg gcgcgtgggt cgtcgtcgcg ccgttgccgc
     4261 tcgtcggcgg c
//
LOCUS       DATMSX010000303         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_118440,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000303 DATMSX010000000
VERSION     DATMSX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118440"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VN041_09210"
     CDS             complement(<1..968)
                     /locus_tag="VN041_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29253.1"
                     /translation="MNDVIYRPGEWQVVVEDAGIAALPADASADKVVALSEMLRRGVP
                     ALTEVIDVLSGGAITGLGSFAVALIGAGGTRFAVRGAVTVTTGLDEGFSGEDITTWSE
                     RYIAGEPRFEIRIGDAPDAVEYPIRSGVVLTSGIRVGEPFTAPEPEAVAEDVPEEDAD
                     AALVVDGEQADDAPDADAEALEHAGDEPAAQLEGETTSDGEQTDEQTDDVAEPEDASD
                     FASEDASSEDAQVAEPESAEEPASEPSALDADGTGVSETITPDQMLLARAAADAASEQ
                     AAPAEEPDLGATIRPDVAATIAPPAADEDSPHVAETMAGFVTPIPPA"
     gene            complement(965..1783)
                     /locus_tag="VN041_09215"
     CDS             complement(965..1783)
                     /locus_tag="VN041_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWU29254.1"
                     /translation="MTAPILTASGRSTDVGARRKLNEDSHLANAPLFIVADGMGGHDA
                     GEVASATVIEHFSALVGQDALSIAQVRDTLASAREAVGAIGQHGSAGAGTTLSGVAVA
                     TVDGLGYWLVLNIGDSRTYQFSNGELEQITVDHSYVQELIEEGEITPEQANTDPRRNI
                     ITRAIGAGSVGDADYWLFPAERGDRMLVCSDGLTTEVSDERIAEVLGSERDPQRAAEI
                     LTADAVRAGGRDNVTVVIVDALTVASVRGIHVQADEDEDLADTRPRAAADGGMR"
     gene            complement(1780..>2254)
                     /locus_tag="VN041_09220"
     CDS             complement(1780..>2254)
                     /locus_tag="VN041_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29255.1"
                     /translation="LASAPQDATPAAVPVTAENPVAPMAAPAPSAPLVTDPDEDDIES
                     TRISVPGHRLVFTWDDGQRATVSGRTVFGRNPEERDGAVNVAVRDETRSLSKTHFEAG
                     ADARGGWVMDRQSTNGTVIVREGVRIACPPGTRVPVRLGDALEIGDRIVTIGGYV"
BASE COUNT          288 a          809 c          814 g          343 t
ORIGIN      
        1 gccggcggga tcggcgtgac gaagcccgcc atggtctcgg ccacatgcgg ggagtcctcg
       61 tccgctgcgg gaggcgcgat cgtggcggcc acgtcgggcc tgatcgtcgc acccaggtcg
      121 ggctcctcgg cgggcgcggc ctgctcggat gcggcgtcgg ctgccgcgcg cgccagcagc
      181 atctggtccg gggtgatcgt ctccgaaacc cccgtgccgt cggcgtcgag ggcggaaggc
      241 tcggatgccg gctcctcggc ggattcgggt tcggcgacct gcgcgtcctc ggacgacgca
      301 tcctcggacg cgaagtccga agcatcctcc ggctcggcga cgtcatccgt ctgctcgtcc
      361 gtctgctcgc cgtcggaagt cgtctcgccc tcgagctgtg ctgccggctc gtccccggcg
      421 tgctcgagcg cctctgcgtc ggcgtcgggg gcgtcatccg cctgctcgcc gtcgacgacg
      481 agagcggcgt cagcgtcctc ctccgggacg tcctccgcaa cggcctcggg ctcgggggcc
      541 gtgaacggct cccccacccg gatgcccgag gtgagcacca cgcccgagcg gatcgggtac
      601 tccacggcat ccggagcgtc gccgatgcgg atctcgaacc gcggctcacc cgcgatgtag
      661 cgctcgctcc acgtggtgat gtcctcgccg gagaagcctt cgtcgagccc ggtggtcacg
      721 gtcaccgcgc cgcgcacggc gaagcgcgtg ccgccggcgc cgatcagggc gaccgcgaac
      781 gagccgaggc cggtgatggc gccgccggag agcacgtcga tgacctcggt cagtgcgggg
      841 acgcccctgc gcagcatctc ggacagcgcc acgaccttgt cggcgctcgc gtcggcgggc
      901 agcgcggcga tgccggcgtc ctcgacgacg acctgccact caccgggacg atagatcacg
      961 tcattcatcg cattcctcca tccgccgccg cgcgcgggcg cgtgtcggca aggtcctcgt
     1021 cctcatcggc ctgcacgtgg atgccccgca ccgatgcgac cgtcagtgcg tcgacgatca
     1081 cgaccgtgac attgtcacga ccgcccgctc gcacggcatc cgcggtgagg atctccgcgg
     1141 cgcgctgcgg gtcgcgctcg ctgcccagca cctcggcgat gcgctcgtcg gacacctcgg
     1201 tggtcagccc gtcggagcag accagcatcc ggtcgccgcg ctcggcgggg aagagccagt
     1261 agtcggcgtc accgacgctg cccgcgccga tcgcacgcgt gatgatgttg cgccgagggt
     1321 cggtgttggc ctgctcgggg gtgatctcac cctcttcgat cagctcctgc acgtaggagt
     1381 ggtcgacggt gatctgctcg agctcgccgt tcgagaactg gtacgtgcgg gagtcgccga
     1441 tgttcagcac cagccagtag ccgagtccgt ccaccgtggc gacggcgacg ccgctgagcg
     1501 tcgtgcccgc gcccgcggag ccgtgctggc cgatggcgcc gaccgcctcc cgagcggatg
     1561 ccagggtgtc gcgcacctgc gcgatcgaga gcgcgtcctg gccgaccagg gctgagaagt
     1621 gctcgatgac cgtggcgctg gccacctcgc cggcgtcgtg accgcccatg ccgtcggcga
     1681 cgatgaacag cggcgcgttc gccaggtggg agtcctcgtt gagcttgcgg cgggccccca
     1741 catcggtgga gcggccgctc gcagtcagga tcggcgcggt cacacgtaac ctccgatggt
     1801 gacgatgcgg tcgccgatct cgagcgcgtc gcccagacgc acgggaaccc gagtgccagg
     1861 ggggcacgcg atgcgcacgc cctcgcgcac gatcacggtc ccgttggtcg actgacggtc
     1921 catcacccag ccgccgcggg cgtccgcgcc ggcttcgaag tgcgtcttgg acagcgaacg
     1981 ggtctcgtcg cggacggcga cgttgaccgc accgtcgcgc tcctcggggt tgcgtccgaa
     2041 cacggtgcgc ccggagacgg tggcacgctg cccgtcgtcc caggtgaaca ccaggcggtg
     2101 accggggacg ctgatgcggg tggactcgat gtcgtcctcg tcggggtccg tgacgagcgg
     2161 tgcggacggg gccggcgctg ccatcggcgc gaccgggttc tccgccgtca cggggacggc
     2221 ggcgggcgtc gcatcctgcg gcgcggatgc cagt
//
LOCUS       DATMSX010000304         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_42756,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000304 DATMSX010000000
VERSION     DATMSX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42756"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VN041_09225"
     CDS             complement(<1..555)
                     /locus_tag="VN041_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29256.1"
                     /translation="MHSLDDIMRASAAWVWFPRGGDQERAELQLVRYPARFGGGVKAS
                     MVQSGLDAEGVLEHALARTRSWGEPRLTFWTNASDSPDLEAELQRRGAEHVDTVAVFA
                     RPIDGLVVEVPEGVTAEIVRTLEQVREVDAVNVPVWDQHPLDEAGLQEELAEVTAGLE
                     SGTGARVLARLDGAAVSTGGCTVVD"
     gene            complement(587..>1910)
                     /locus_tag="VN041_09230"
     CDS             complement(587..>1910)
                     /locus_tag="VN041_09230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU29257.1"
                     /translation="EDWAASVHHAAPGFPADAATDILRRTAPRTVSGAGLPDAGDDRI
                     DAIVRGVLDLDDSYLAVQGPPGTGKTYTGSQVIARLVNEHGYRVGVVAQSHSIVENLL
                     ERVVADGVPASKVAKKPKDEEAPAAYTVIRKDGMAAFLAEHAQGGAVVGGTAWDFSNT
                     RRVDRKGLDLLVIDEAGQFSLASTIAVAAGAKRLLLLGDPQQLPQVSQGTHPEPVNTS
                     ALGWVMDGDSVIDPAYGYFLAESWRMHPEVAAPVSTLSYAGRLASAPGAEGRIVEGVE
                     PGLHIVPVRHRGNATQSPEEAAEVVRIVRDLVGRGYVDGMEDAAQRPLDQSDIIVVAP
                     YNAQKQLIHDELAAAGFGGVAVGTVDNFQGKEAVVSITSLAASSGRDAPRGPEFLLLQ
                     NRLNVAISRAKSVAYLVHSPALLDDLPWTPEGVARLSAFAHLVGADRD"
BASE COUNT          266 a          713 c          643 g          288 t
ORIGIN      
        1 gtcgacgacg gtgcagccgc cggtgctgac cgcggctccg tccaggcggg ccagcacccg
       61 cgcgccggtt ccggactcca gccccgcggt gacctcggcg agctcctcct gcagacctgc
      121 ctcgtcgagc ggatgctgat cccacaccgg cacgttcact gcatccacct cgcgcacctg
      181 ctcgagcgtc cgcacgatct ccgcggtgac gccctccggc acctcgacca cgagaccgtc
      241 gatcggcctc gcgaacacag cgaccgtgtc gacgtgctcg gcgcccctgc gctgcagctc
      301 ggcctcgagg tccgggctgt cggaggcgtt cgtccagaac gtcagcctcg gctctcccca
      361 ggagcgggtg cgcgcgaggg cgtgctccag cactccctcg gcatccagtc ctgactgcac
      421 catcgaggcc ttcactccgc caccgaaccg ggccgggtaa cgcacgagct gcagctcggc
      481 cctctcctgg tcgccgccac gcgggaacca gacccatgcc gctgaggccc gcatgatgtc
      541 gtcgagactg tgcatgctag caatctaaca tcttgcgtcc gagcgctcag tcgcggtcgg
      601 ccccgaccag atgggcgaag gcgctcagtc gcgcgacgcc ctccggcgtc cagggcaggt
      661 cgtcgagcag agccggcgag tgcacgaggt aggcgacgct cttggcgcgg gagatcgcga
      721 cgttgaggcg gttctgcagc agcaggaact cgggcccgcg cggcgcgtcc ctgccgctgg
      781 acgctgccag cgacgtgatc gagacgaccg cctccttgcc ctggaagttg tccaccgtgc
      841 cgacggccac tcccccgaag cctgcggcgg cgagctcgtc gtggatgagc tgcttctgcg
      901 cgttgtaggg cgccaccacg atgatgtccg actggtcgag gggccgctgc gcagcatcct
      961 ccatcccatc cacgtatccg cgaccgacga ggtcgcgcac gatgcggacc acctcggccg
     1021 cctcctcggg cgactgcgtc gcgttgccgc ggtgtcgtac ggggacgatg tgcagccccg
     1081 gctcgactcc ctcgacgatg cgcccctcgg ccccgggagc cgaagccagg cgtcccgcgt
     1141 aggacagcgt cgagacgggg gctgccacct ccggatgcat gcgccaggac tcggcgagga
     1201 agtacccgta cgccggatcg atgaccgaat cgccgtccat cacccagccc agtgccgacg
     1261 tgttcaccgg ctcgggatgc gtgccctggc tcacctgcgg cagctgctgc gggtcgccga
     1321 gcagcagcag gcgcttcgcg cccgcggcga ccgcgatcgt ggaggccagc gagaactgtc
     1381 cggcctcgtc gatcacgagc aggtcgagcc ccttccggtc gacgcgccgc gtgttgctga
     1441 agtcccaggc cgtaccgccg accaccgccc cgccctgggc gtgctcggcg aggaacgcgg
     1501 ccatgccgtc cttgcggatg acggtgtagg ccgccggggc ctcctcgtcc ttgggcttct
     1561 tggcgacctt cgacgccgga accccgtccg ccaccacccg ctccagcagg ttctcgacga
     1621 tcgagtgcga ttgcgcgacg actccgacgc ggtagccgtg ctcgttcacc agccgcgcga
     1681 tgacctgcga gccggtgtag gtcttgccgg tgcccggtgg gccctgcacg gcgagatagc
     1741 tgtcgtccag gtcgaggaca ccgcgcacga tcgcgtcgat gcggtcgtcg ccggcatccg
     1801 gcagtcccgc acccgacacg gtgcgcggcg cggtcctgcg gaggatgtcg gtggccgcat
     1861 cggcggggaa gccgggtgcg gcgtgatgca cgctcgccgc ccagtcctcg
//
LOCUS       DATMSX010000305         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_5021,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000305 DATMSX010000000
VERSION     DATMSX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5021"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN041_09235"
     CDS             complement(<1..249)
                     /locus_tag="VN041_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29258.1"
                     /translation="MSSKHRDPEYLRNARIVRAQVRRAWRLGTEVRCWRCGRVIEPGM
                     PFDVGHLDPDGGHGRSNLAPECVPCNRRDGGRMGAAVTN"
     gene            complement(532..1836)
                     /locus_tag="VN041_09240"
     CDS             complement(532..1836)
                     /locus_tag="VN041_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HWU29259.1"
                     /translation="MPSFLHPDTGRFDATLLADHIAHSPTKLGRDFAGNLYEYRDGVY
                     VRDERVVTRRCAAALKSSYTRNVETQAAAHLLNIDLPEVGLPELPRGYLDYVVLENGV
                     YWWAEDRLTPHNEMLGALTKLPITYDETALPHEFKTWLVQVFGDDDELIRHVWEVLGY
                     LLMTGNPLQKIIMLFGGGGDGKGTLMRLIRAMLGKSNYSSVSMHQLVEDRFSSANLYG
                     KIANISGDLSSAFLRDPQILKEITGGDSINAAYKHGQAFEFVPYAVPLFAANEFFRTS
                     DNSYGWRRRWMVIEFPNKVEAEGATGPLYDESVLHDEIPAIFNYAMEGLRRLMTRGRF
                     LPPDAVQEATTRMHDEADPFLTWLDEDDAVWVGEDQSSPKEDVYKAYAAWSRRNGYQV
                     MNHSSLAQRLKQRGVTATRPRVGGSRVHYYQGIGVTLSTVTG"
     gene            complement(1836..2180)
                     /locus_tag="VN041_09245"
     CDS             complement(1836..2180)
                     /locus_tag="VN041_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29260.1"
                     /translation="MPECRADATIPAPWPLVTSDDFHRRRPGEPQGYVPAEGDRIRYA
                     LRHTHWCPAAGCMVAGHDEPEEFRGVVTVIGAFERDGETRYTLRHAGGDLSWIDLDWW
                     ITEHVLEDGALW"
     gene            complement(2173..2358)
                     /locus_tag="VN041_09250"
     CDS             complement(2173..2358)
                     /locus_tag="VN041_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29261.1"
                     /translation="MADRWQDVADIIGAYDAGMDAEDLGEAGQAWARQRFGGEARYLV
                     KVFDYLMAAREVGSSDA"
     gene            complement(2351..2527)
                     /locus_tag="VN041_09255"
     CDS             complement(2351..2527)
                     /locus_tag="VN041_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29262.1"
                     /translation="MAEFEARITVYKDGQELMFNDALGDSASDALYAAHHDLWNDIVR
                     MESSTNREDGSNHG"
     gene            complement(2520..2966)
                     /locus_tag="VN041_09260"
     CDS             complement(2520..2966)
                     /locus_tag="VN041_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29263.1"
                     /translation="MAEHDHITKTDTLGCPACDKLEGRDRVPAPMPEQPTMPDLSAFP
                     SAQHRIEGGKFICPGDENAWCHQYPEDDCDHEYWPCGHEFTSHAECWIKPWIDATDLA
                     DSYAGVEHELVLRDEDFPDGDVDWTWEGEYVTFDYAPDRENGSDHG"
     gene            complement(3292..3654)
                     /locus_tag="VN041_09265"
     CDS             complement(3292..3654)
                     /locus_tag="VN041_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29264.1"
                     /translation="MARRSENNALTFLRCGECTAGVADITGAISVRHNPDGTHALIFP
                     DPRPDHGQLSVWLDVKAALNHVHQVQFYLGYLPEKPQAMWDHEAIRMQLDDARAVLER
                     LVDGLWDQTSWPRDEAGS"
     gene            complement(3624..3884)
                     /locus_tag="VN041_09270"
     CDS             complement(3624..3884)
                     /locus_tag="VN041_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29265.1"
                     /translation="MGDRTHRTFPVGSTRPHISHETWLYMRVRIEHLEAEVSRLTVDR
                     DHWYAAANYTPEELHEQFMRASQGRDPETGEWLWPDGVRTTR"
     gene            complement(3889..4017)
                     /locus_tag="VN041_09275"
     CDS             complement(3889..4017)
                     /locus_tag="VN041_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29266.1"
                     /translation="MSRRRTYVPHVTRREHLAAALGAVVGMLAISLVVVVFLCAAR"
     gene            complement(4014..4217)
                     /locus_tag="VN041_09280"
     CDS             complement(4014..4217)
                     /locus_tag="VN041_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29267.1"
                     /translation="MKTTDSRDSDWLKPGEAASALGVSVDTVARLADKGEIRAIRPGG
                     GHRRYAAADVEAIVAGEPWEPAS"
     gene            complement(4589..4661)
                     /locus_tag="VN041_09285"
     tRNA            complement(4589..4661)
                     /locus_tag="VN041_09285"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4626..4628),aa:Asn,seq:gtt)
     gene            complement(4751..5515)
                     /locus_tag="VN041_09290"
     CDS             complement(4751..5515)
                     /locus_tag="VN041_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU29268.1"
                     /translation="MPASDAAIDCSDLVIDRIGHGGVTRAVDGVTFTVRAGELMCVGG
                     ATGAGKSTLVSALAGYGDSSLKVAGGRARVCGVDIRRPGRRRRMLTALSGYIPQSAGA
                     NLPPRLTVGEVISEPITSREKRVNSKALAIRVASLLDELHLPLGAAAKFPYELSAGMR
                     QRVAIARAFMLEPHVLIADEPLANLDLEVRPVVFDAITRRRNEQSMAALLVTNDAAFI
                     RELDAEALILRSGHVVARGIGKDLLWSPNSEADAAR"
     gene            complement(5550..>6016)
                     /locus_tag="VN041_09295"
     CDS             complement(5550..>6016)
                     /locus_tag="VN041_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWU29269.1"
                     /translation="VRLADLPRTADVAIERDALMGMLQYGHQIDQALLSRAVAEQFRH
                     PGLDAVREAIAAAPDRTRVGWATTAADAVREPYRSLAGELLMMPFPAKDEERALVSAT
                     DLCRRLLLRAIEREKNELLGAVQRVPADSDGGRALRVRLRDLDQERLRFVES"
BASE COUNT          936 a         2114 c         1962 g         1004 t
ORIGIN      
        1 gttcgtgacg gcggcaccca tccggccacc gtcgcgacgg ttgcacggca cgcactcggg
       61 tgcgaggttc gaccggccgt gaccaccgtc cgggtcgagg tgcccgacgt cgaacggcat
      121 gcccggctcg atcactcgac cgcagcgcca gcaccgaacc tcggtgccga gccgccaggc
      181 acgacgcacc tgtgcacgca cgatccgggc gttgcgcagg tactcgggat cgcgatgctt
      241 gctcgacacg atgagactcc gatccggtcc acgtggtcag cccggtcagg gcagtggccc
      301 tacttctctc tgttcttttc tttgcgcgag agtaagtaga gatagggctg accgggctga
      361 ccagcccgcc ccccgcctgc gatgcagggc tgaccacagg cctgaccgca ccctgaccac
      421 agggctgacc acctcggtgg tcagagcgaa gctcctcatt ccgtggggcg cggggagcgg
      481 ggcgtgcggc cctggcctga ccaccggtca gccctgacca caccacggcg ctcacccggt
      541 caccgtcgag agtgtgacgc cgatgccctg gtagtagtgc acgcgggagc cgccgacgcg
      601 gggacgggtg gcggtgacgc cgcgctgctt gagccgctgc gccagggacg agtggttcat
      661 cacctggtag ccgttgcggc gcgaccaggc ggcgtaggcc ttgtagacgt cttccttcgg
      721 gctggactga tcctcaccca cccacacggc gtcgtcctcg tcgagccagg tcaggaacgg
      781 gtcggcctcg tcgtgcatgc gcgtggtggc ttcctggacg gcatccggtg gcaagaaccg
      841 gccacgggtc atcagccggc gcagaccctc catcgcatag ttgaagatcg ccggtatctc
      901 gtcatgcagc accgactcgt cgtacagcgg cccggtggcg ccctcggcct cgaccttgtt
      961 cgggaactcg atcaccatcc accgacgccg ccacccgtag ctgttgtcgg aggtgcggaa
     1021 gaactcattc gcggcgaaca gcggcaccgc gtacggcacg aactcgaacg cctgcccgtg
     1081 cttgtacgcg gcgttgatcg agtcgccgcc ggtgatctcc ttgaggatct gcgggtcgcg
     1141 caggaacgcg gacgagaggt cgccgctgat gttcgcgatc ttgccgtaga ggttcgccga
     1201 tgagaaccgg tcctcgacga gctggtgcat gctcacgctc gagtagttcg acttgccgag
     1261 catcgcccgg atgaggcgca tcagcgtgcc cttgccgtca ccaccaccgc cgaacagcat
     1321 gatgatcttc tgcagcgggt tgcccgtcat cagcaggtag ccgagcacct cccacacgtg
     1381 ccggatcagc tcgtcatcgt caccgaacac ctgcacgagc cacgtcttga actcgtgcgg
     1441 cagcgcggtc tcgtcatagg tgatcgggag cttggtcagc gcgccgagca tctcgttatg
     1501 cggggtcagg cggtcctcgg cccaccagta gacgccgttc tcgagcacca cgtagtcgag
     1561 gtagccgcgc gggagctcgg gcagaccgac ctcgggcaga tcgatgttca gcaggtgcgc
     1621 ggccgcttga gtctcgacat tgcgggtgta cgacgacttc agcgccgcag cgcaccggcg
     1681 agtcaccacc cgctcgtcac gcacgtacac gccgtcccgg tactcgtaga ggttgcctgc
     1741 gaagtcgcgg ccgagcttcg tcggcgagtg cgcgatgtga tccgcgagca acgtcgcatc
     1801 gaaccggccg gtatcgggat gcaggaacga cggcattacc agagcgcccc atcctcgagt
     1861 acgtgctcgg tgatccacca gtcaaggtcg atccaactga ggtcgccgcc ggcatggcga
     1921 agcgtgtagc gtgtctcgcc gtcgcgctcg aacgctccga tgacggtcac gacgccgcgg
     1981 aactcctcgg gctcgtcgtg gccggcgacc atgcaacccg cggccgggca ccagtgtgtg
     2041 tgccgcagtg cataccggat gcgatcgccc tcggcaggca cgtacccctg aggctcaccc
     2101 ggccgccgcc ggtggaaatc gtccgaggtc acgagcggcc acggtgccgg gatcgtggca
     2161 tccgccctgc actcaggcat ccgaactccc aacctcgcga gcggccatca ggtagtcgaa
     2221 caccttgacc agatagcggg cttcgccgcc gaagcgttgg cgcgcccagg cttgacccgc
     2281 ttcgcccagg tcttcggcat ccatcccggc gtcgtatgcg ccgatgatgt cagcgacgtc
     2341 ctgccagcga tcagccatgg ttgctcccgt cctcgcggtt ggtactgctc tccatccgca
     2401 cgatgtcgtt ccacaggtcg tggtgtgcgg catagagcgc gtcggatgcg gagtcaccca
     2461 gcgcatcgtt gaacatcagt tcctggccgt ccttatagac cgtgatccgc gcctcaaact
     2521 cagccatggt cgctcccgtt ctcgcggtct ggggcgtagt cgaaggtgac gtactcgcct
     2581 tcccacgtcc agtccacgtc gccatccggg aagtcctcgt cgcgcagcac cagctcgtgc
     2641 tcgacgcctg cgtacgagtc ggccagatcg gtcgcgtcga tccacggctt gatccagcac
     2701 tcggcgtggc tggtgaactc gtggccgcac ggccagtact cgtgatcgca gtcgtcctcc
     2761 gggtactggt ggcaccatgc gttctcgtcg ccggggcaga tgaacttgcc gccctcgatg
     2821 cggtgctgcg ccgaggggaa cgccgacagg tcgggcatcg tcggctgctc cggcatcggt
     2881 gcgggcactc ggtcgcgacc ttccagcttg tcgcacgcgg ggcacccgag agtgtcggtc
     2941 ttcgtgatgt ggtcgtgctc agccatcgct cttgctcccg tgcacgtgca cggatgctcc
     3001 gggcacgtgc ggccctcgcg ggcgtcgtgc tgggacaggt gctcgcatgg ctgagggcag
     3061 atctcgcgat ctgtgtcccg ccattcgtcg tcacgctcgt gtccgcgaag gatgccgagc
     3121 acggcgcacg gacatccgcc gtcgcccgcc cagttcgggc acagctcctg gtcgcatccg
     3181 gtcatgactc gctcccgttc tcgcggtctg tagcacgggc atcgaccctg caggcgaccc
     3241 gccaggcgac gtagaggaac gctccggcga ggatccatgc accgttgatg atcatgagcc
     3301 ggcctcgtct ctcggccacg aggtctgatc ccagagcccg tcgacgagac gctcgagcac
     3361 ggcgcgcgcg tcgtcgagct gcatccggat cgcctcgtga tcccacatcg cctgcggctt
     3421 ctcgggcagg tagccgaggt agaactgcac ctggtgcacg tggttcagcg ccgccttgac
     3481 gtcgagccac accgacagct gaccgtgatc cggccgcggg tccgggaaga tcagcgcgtg
     3541 cgtgccgtcc gggttgtgcc ggacgctgat cgcacccgtg atgtccgcga cgcctgcggt
     3601 gcactcacca caccgcagaa acgtcagcgc gttgttctca ctccgtctgg ccatagccat
     3661 tcccctgtct ctgggtctcg tccttgggat gcccgcatga actgctcgtg cagctcctcg
     3721 ggcgtgtagt tcgccgccgc gtaccagtgg tcgcggtcga ccgtgagccg ggacacctcc
     3781 gcctcgaggt gctcgatccg gactcgcatg tacagccagg tctcgtggct gatgtgcggg
     3841 cgggtggagc cgaccgggaa cgtgcggtga gtccggtcgc ccacgacgtc accgggccgc
     3901 acacaggaac accaccacga ccaggctgat cgcgagcatc ccgaccacgg cgccgagcgc
     3961 agcggccagg tgctcgcgcc gcgtcacgtg cggcacgtac gtgcgccggc ggctcacgag
     4021 gccggctccc acggctcacc cgcgacgatc gcctcgacat cggcggcggc gtagcgtcga
     4081 tgaccaccgc ccggtcggat agcgcgaatc tcgcccttgt cggcgaggcg agcgactgtg
     4141 tcgacgctga caccgagcgc ggaagcggcc tcgcctggct tgagccaatc ggaatcccga
     4201 ctatctgtcg tcttcatggt tatgcaccat acccgacaat cagtcggata tcgcaaaacc
     4261 cgtagttctc ttgcgtgttt gcgggattta ctggatactc gtgggtatgc agacgacacg
     4321 ccagcgaaac acgccgagcc gcgacatcga cgccgagatc ggtcgtcgcg ctcacatgct
     4381 catgtgggat gccaagcgga agcagggtga ggtagccgag cagatcggca tccactcgga
     4441 ttccctgagc cgcaagctca agggcaccac tggatgggcg gcatccgagc tcgtcgcgat
     4501 cgcgggtgtg ctcggaacgt cggtcagtta cctggtgggg gaagcagaag acccccaccc
     4561 tggtatgggt gggggtcttc ggggatcgct ccccggcttg gactcgaacc aagaacctgc
     4621 cggttaacag ccggctgctc tgccaattga gctaccgagg atcatgttcc ccgctgtgcg
     4681 ggcaactcat ccagcttagc aaactccccg ccgtgctccg aacacgggcg gcgtcccggg
     4741 cgtggcgcgt tcagcgcgcg gcatccgcct cggagttggg tgaccagagc aggtccttgc
     4801 cgatcccccg agccaccacg tgcccgctgc gcaggatcag cgcctctgcg tcgagctccc
     4861 ggatgaaggc tgcgtcgttg gtgaccagca gcgccgccat cgactgctcg ttgcggcggc
     4921 gcgtgatcgc gtcgaacacc acggggcgca cctcgaggtc gaggttggcg agcggctcgt
     4981 ctgcgatcag cacgtgcggc tccagcatga aggcccgggc gatcgcgacg cgctggcgca
     5041 tgcccgcgct gagctcgtag gggaacttcg ccgcagcccc cagcggcagg tgcagctcgt
     5101 ccagcagcga cgcgaccctg atcgcgagcg ccttgctgtt gacccgcttc tcgcgcgagg
     5161 tgatcggctc cgagatgacc tcgccgaccg tgagccgcgg gggcaggttc gcccccgcgc
     5221 tctgcgggat gtacccgctc agcgcggtca gcatccggcg gcggcgcccg ggcctgcgga
     5281 tgtcgacccc gcacaccctc gcacgaccgc cggcgacctt cagcgacgag tcgccgtacc
     5341 cggcgagagc cgacacgagc gtcgatttgc cggcgcccgt cgcgccgccg acgcacatca
     5401 gctcgcccgc ccggaccgtg aacgtgacgc cgtccacggc gcgcgtcacg ccaccgtgcc
     5461 cgatccgatc gatgacgagg tcggagcagt cgatggcggc gtcactggcg ggcatgcctc
     5521 catcctgcct gctgaacccg ctcagacgct cacgactcga cgaagcgcag ccgctcctga
     5581 tccagatccc gcagtcgcac ccgcagcgcc cgaccgccgt cggagtcggc gggtacgcgc
     5641 tgcacggcgc ccagcagctc gttcttctcc cgctcgatcg cccgcagcag caggcgccgg
     5701 cacagatccg tcgccgatac cagcgcgcgc tcctcgtcct tcgcggggaa cggcatcatc
     5761 agcagctccc ccgccaggct ccgatacggc tcgcgcacgg cgtccgccgc ggtcgtcgcc
     5821 cagccgaccc gggtccggtc cggggcggcg gcgatcgcct cacgcacggc gtcgaggccc
     5881 ggatgccgga actgctccgc cacggcgcgc gagagcagcg cctgatcgat ctgatgcccg
     5941 tactgcagca tgcccatcag cgcgtcccgc tcgatcgcga cgtccgccgt gcgcgggagg
     6001 tcggccagac gcaccc
//
LOCUS       DATMSX010000306         1920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_184586,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000306 DATMSX010000000
VERSION     DATMSX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184586"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VN041_09300"
     CDS             complement(<1..480)
                     /locus_tag="VN041_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWU29270.1"
                     /translation="MTSPRSLLDGFARLYPASDGPLHGSALLWAHGGGFVHGDLDMPE
                     ADAVARAFADRGATVVSVDYALVGDDGSRTFPAGSDDILTGWAWLVEHAQELGAERLF
                     AGGTSAGGNLIAGTVLRLLGHAPASDLPLPNGVFLAYPTMLAVQPAPDAELRAALDAN
                     "
     gene            549..1208
                     /locus_tag="VN041_09305"
     CDS             549..1208
                     /locus_tag="VN041_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29271.1"
                     /translation="MMLERITGLESSRVADALRDDIMLGRREPGSRLIERDIAAELQV
                     SRLPVREAIKALVADGIVVARPRSWAVVREFSVQDLQDFAEVRSAMETLAFVLATQRA
                     DESALALLESLIDDEEASARSGDAVASRQASAMFHITAVELAQNAMLTELAASLVTRL
                     RWLFGQHDELEEMAVQHRDILRAMRERDVEAIKVMIPAHLDEGRLAAEQRLRARHDEN
                     G"
     gene            1261..>1920
                     /locus_tag="VN041_09310"
     CDS             1261..>1920
                     /locus_tag="VN041_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29272.1"
                     /translation="MIPALRTFARILARHWPALIAWYVGGEVLHRLLVQLAGFVGGYS
                     TLGGLLLLPLAVGARLAAYVAMYLTVRPSLAHVAQSGEPDAGIRAFLRSTLVAILPFS
                     IFYAAWGMLDADQTEFFRIASAIAFRETGFGLGEAIVDRGGLISVGVLPVTVLVVALL
                     VRALHARFRARLPEWTLTVAAYAELLWTFMLFTLVGQWLAGVRAWFAGRAGALWLQDF
                     GD"
BASE COUNT          247 a          674 c          701 g          298 t
ORIGIN      
        1 gttcgcgtcc agcgccgccc gcagctcggc gtcgggcgcg ggctgcacgg ccagcatggt
       61 cgggtaggcc aggaacacgc cgttcggcag ggggagatcg gatgccggtg cgtgccccag
      121 cagccgcagc acggtcccgg cgatcaggtt gccgccggcg ctggtgcccc cggcgaacag
      181 cctctcggcg ccgagctcct gtgcgtgctc gaccagccac gcccatccgg tgaggatgtc
      241 gtccgacccg gccgggaacg tgcgcgagcc gtcatcgccg acgagggcgt agtccaccga
      301 gacgaccgtg gcgccccgat ccgcgaacgc ccgggccacg gcatccgcct ccggcatgtc
      361 cagatccccg tgcacgaagc caccgccgtg cgcccacagc agagccgatc cgtgcagcgg
      421 gccgtcggac gcggggtaga ggcgggcgaa accatcgagc aggctgcggg gagacgtcat
      481 gcacccagaa tggcacccgt ttcaccggaa cactggccgc aagtcgcacc gggtggttga
      541 atccaggaat gatgctggag cggatcaccg ggctggagtc gtcacgtgtg gcggatgccc
      601 tccgcgacga catcatgctt ggccggcgcg agcccggttc gcggctgatc gagcgcgaca
      661 tcgccgccga gctgcaggtc tcccggcttc cggtgcggga ggcgatcaag gctctggtcg
      721 cggacggcat cgtcgtggcg cgcccacgca gctgggcggt cgtgcgggag ttctcggtgc
      781 aggacctgca ggacttcgcc gaggtgcgct cggcgatgga gaccctcgcc ttcgtgctgg
      841 ccacgcagcg cgccgacgag agcgccctcg ccctgctgga gtcgctcatc gacgacgagg
      901 aggcgtccgc gcggtcgggc gacgcggtcg cgtcgcgcca ggcgtccgcg atgttccaca
      961 tcaccgccgt ggagctggcg cagaacgcca tgctcaccga gctcgccgcc tctctcgtca
     1021 cccggctgcg ctggctgttc ggccagcacg acgagctgga ggagatggcg gtccagcacc
     1081 gtgacatcct ccgtgccatg cgcgagcgcg acgtcgaggc catcaaggtc atgatccccg
     1141 cccacctgga tgaggggaga ctcgccgccg agcagcggct gcgtgcccgc cacgacgaga
     1201 acggctgacg ccggcgtgaa cgctgccgtg cttcccccgc gcagccgata ccgtgggggc
     1261 atgatcccgg cgctgcgcac cttcgcgagg atcctcgcgc gccactggcc ggcgctgatc
     1321 gcctggtacg tgggcggcga ggtgctgcac cggctgctcg tgcagctggc cggcttcgtc
     1381 ggcggctaca gcacgctcgg cggcctgctc cttcttccgc tcgcggtcgg cgcgcgtctg
     1441 gccgcgtacg tggccatgta cctgacggtg cgcccctcgc tcgcgcatgt ggcgcagagc
     1501 ggggagcccg atgcaggcat ccgcgcgttc ctgaggtcga cgctcgtcgc gatcctgccg
     1561 ttctcgatct tctacgcggc gtgggggatg ctcgacgccg atcagacgga gttcttccgc
     1621 atcgcgagcg ccatcgcgtt ccgcgagacg ggcttcggtc tcggggaggc gatcgtcgat
     1681 cgaggcgggc tcatctccgt gggcgtgctg ccggtgacgg tgctcgtggt ggctctgctg
     1741 gtccgcgccc tgcatgcccg gttccgcgcg cgcctgccgg agtggaccct gacggtggcc
     1801 gcctatgcgg agctgctgtg gacgttcatg ctgttcaccc tggtcggcca gtggctcgcc
     1861 ggcgtgcgcg cgtggttcgc cggccgggcg ggggccctgt ggctgcagga cttcggcgac
//
LOCUS       DATMSX010000307        12738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156414,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000307 DATMSX010000000
VERSION     DATMSX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156414"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VN041_09315"
     CDS             complement(<1..430)
                     /locus_tag="VN041_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWU29273.1"
                     /translation="MPVWRFDAIGTRWEIESSRELTDDERGAATAVIDRFDQEWSRFR
                     PDSVVSSLAGGGSAPLPSDAAAMLDAYRELSEATDGAVNPLIGGSLDALGYDASISLI
                     PSDPQPAPADWRSLLGWDGRLSLAAPAIIDVGALGKGRLVD"
     gene            complement(439..897)
                     /locus_tag="VN041_09320"
     CDS             complement(439..897)
                     /locus_tag="VN041_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWU29274.1"
                     /translation="MIRTTVPSSARKGAAVVGVAGLLVLAGCSGAADAEGSADADMTG
                     SDSTASTSSAEYADGTYTAEGSYQTPETVETISVTLTLADGVVTEVEVTGDPQAPETE
                     QYQGEFIDGIEAEVVGVAIDDLAVDRVAGSSLTSGGFNAAVEDIKEQAAA"
     gene            complement(1048..2082)
                     /locus_tag="VN041_09325"
     CDS             complement(1048..2082)
                     /locus_tag="VN041_09325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWU29275.1"
                     /translation="MARKKKTVELDRDNGIAPGLVAKTKKRLVVAGGRSHPELTRAVA
                     EALGTELAPTEHRTFASGEIYARFEVSIRGCDLFLIQTFGEPVNEWLMETLIMIDAAK
                     RASAKRITVVAPYYPYSRQDKKGRGREPISARLVADLLKTAGADRVMSVDLHAAQIQG
                     FFDGPVDHLFAKPVLLDYFERTLTPEDREILTVVSPDMGRVRVADTWSDSLGAPLAII
                     HKRRDPKVANQVSVHEIVGTVEGRTCLLVDDMIDTGGTIVKAAQALKANGAHRVIVAA
                     THAIFSDPANERLQDSSIDEVVVTDTIPLTESRRWENLTILPIAPLLARAIREVFEDG
                     SVTSMFGGDA"
     gene            complement(2057..3520)
                     /gene="glmU"
                     /locus_tag="VN041_09330"
     CDS             complement(2057..3520)
                     /gene="glmU"
                     /locus_tag="VN041_09330"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWU29276.1"
                     /translation="MTQNNLAIVVLAAGQGTRMRSRLPKVLHPIAGRPLVGHVLTTAR
                     RLGAEHIEVVVRHERDQVVAALAEDYPGTVFVDQDDVPGTGRAVEVALDAIPDFDGDV
                     LILSGDCPLADVDTLTAFVDEHRSTGAAATLMTAVVEDPTGYGRVIRDAAGDVDRIVE
                     QKDATADEAAVSEINAGMYVFRAAELRRYLPTVGVDNAQGEKYLTDVPGLLRQAGDRV
                     AASVVSDVTVTYGVNDRAQLALVGRLLNERIVRSWQLAGVTIVDPATTWIDDDAKLAA
                     DVTILPHTQILGATVIAEGATVGPDTTLVDCEVGADAVVKRTDANLAVIGAGATVGPF
                     SFLRPGTVLGANGKIGAYVETKNAQIGDGSKVPHLSYVGDATIGRGVNLGASTITANY
                     DDVNKHRTEIGDEVHTGSHTVLVAPVRLGAGAKTGAGAVVRKDVPAGALAMSVAPQRN
                     IEGWVEKNRAGTGAADAASRAHDAAEKAGDGAQEEDR"
     gene            complement(3522..3593)
                     /locus_tag="VN041_09335"
     tRNA            complement(3522..3593)
                     /locus_tag="VN041_09335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3559..3561),aa:Gln,seq:ttg)
     gene            3716..4216
                     /locus_tag="VN041_09340"
     CDS             3716..4216
                     /locus_tag="VN041_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29277.1"
                     /translation="MSIVIERVDTATPALQAFVAAHHAEMEGTAPPESQHALSFDRLL
                     EPGVRLFAGYHEGRPVATGALAAVDGGHEELKSMRIAPTSRRQGFGRAMLAFLIGDAG
                     SRGIRRLSLETGRDPFFAPARALYADAGFVECEAFGRYLPDPHSAFLTLAPVPVAGME
                     AAPHAG"
     gene            4224..4718
                     /locus_tag="VN041_09345"
     CDS             4224..4718
                     /locus_tag="VN041_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29278.1"
                     /translation="MSEVDEVDRIVSAWNAQRPDLDFSPLEVLSRMDRLSRHLDRARR
                     EVFHRSDIEPWEWDVLSALRRAGSPFQLSPKQLLQQTLVTSGTMTNRIDRLVGRRFVR
                     READPGDGRSILVILTDDGRVRVDAAITRLVDAEAELLQALPRADRDRLAGLLRKLSL
                     SFDV"
     gene            4735..5676
                     /locus_tag="VN041_09350"
     CDS             4735..5676
                     /locus_tag="VN041_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWU29279.1"
                     /translation="MTSPLPVRDGVGATRLHLPLSGPWPTIGAYMAERFFHLDPERLL
                     TRFDRGEIVARDGSPLARDTPLGEHEFVWYYREPPVEREIPFEIEVLHQDRDLVVADK
                     PHFLPTTPGGKYLQNSALVRLRNLLGIPELTPIHRLDRATAGLLMFSVRPETRGAYQL
                     LFENRRVEKVYEAVSALPEGWDAASPALDGRPFPIVHRNHIEKLRANVCVRVDGDREP
                     NSETLIELIDADESVLHTRLRPHSGKMHQLRVHLAALGAGILNDGFYPVLLPEAPDDF
                     TKPLQLLARELRFTDPLTGAAREFVTRRTLQEAPTGA"
     gene            complement(5663..6322)
                     /locus_tag="VN041_09355"
     CDS             complement(5663..6322)
                     /locus_tag="VN041_09355"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HWU29280.1"
                     /translation="MDRLIELGDEFWKAAVETLYMTVFALVMAGVLGTLIGVGLYLTR
                     RGGLLPNRFVNGLLELLVNFFRPIPFVIFIAVAMPLTRAVIGVGIGTTAGAFAIGLAA
                     SFAIARIVEQHLVSVSPGVLEAARAMGAGPWRILLTVAIPESLGPLILGYTFIVVALI
                     DMTAMAGLVGGGGLGAFAQIYGFRQFEPVVMWAAIVLIVVFVHLVQLLGTKLARVIMR
                     R"
     gene            complement(6322..7365)
                     /locus_tag="VN041_09360"
     CDS             complement(6322..7365)
                     /locus_tag="VN041_09360"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HWU29281.1"
                     /translation="MPIVSLAGVTKSYPAAGRDSAPVTAVDDVTLDIEQGDVFGIIGY
                     SGAGKSTLVRLINALEPATSGTIHVDGVDITALGERELRRIRGGIGMIFQQFNLFSAK
                     TVKANIAYPLKLAGWSPREIDERVAELLRFVGLADKARAYPEQLSGGQKQRVGIARAL
                     ATRPSILLADEATSALDPQTTHEVLDLLGRVNREQGVTIVVITHEMDVIQTIATKVAV
                     MEKGRVIEQGEVFDVFSRPQNPASQRFVGTVIKGLPSPAEAAALRERHAGRLVTFSFR
                     DGDSSQSQVLLDLAQAGLEFELVYGGINDIRGRAFGHLTLAIRGADATIDEALRGIRQ
                     HAEVTELEQEGTR"
     gene            complement(7462..8367)
                     /locus_tag="VN041_09365"
     CDS             complement(7462..8367)
                     /locus_tag="VN041_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU29282.1"
                     /translation="MSRRITTLAAAAATAAVLIASLVGCASNPASSTDSAKKDDTVKI
                     GVVGKSDAQWPAFVAAAKKEGISVELVDFGSYEQPNPALAEGELDLNQFQHIVYLAQY
                     DKASGKDLQPIGATAIYPLGLYSKKYDSVKDIPKGETVAVPDDASNQARALLVLQSAG
                     LIKLKSGGTIVSDLADVDTDKSKVKVSALEAAVIPTALPDVAAAIINNDFVKDAGLSF
                     KDAIAQDDPTDPNALPYVNIFAARADDVDNPTYKKLVEIFQTDAAVQKGLQESSGDTA
                     VPLKTAAADLQKSLKTVLKDTVIKK"
     gene            complement(8638..9279)
                     /locus_tag="VN041_09370"
     CDS             complement(8638..9279)
                     /locus_tag="VN041_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29283.1"
                     /translation="MLPDSPAHRTRLRLLTAASEEMIECGYAAASLSRIAARLGLTKG
                     ALGHHFPTKQDIAIAVFDRADEIAVQVAADARRLFPDSAMRGCIAYFAGLARAGAADP
                     IAAAWLALYQDRSVPVEIARRGYVTSRDVVAGFLADHLAREGGELRMPPAQATLFLQL
                     LVSGELAIARFLTDYSAQNAVDSFATALTGLGVRDAESVVVDGIRAVWTSSPE"
     gene            complement(9317..11140)
                     /locus_tag="VN041_09375"
     CDS             complement(9317..11140)
                     /locus_tag="VN041_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWU29284.1"
                     /translation="MAHLLGAEALHLEYPTRVVFDSVTLGIEEGDRIGIVGRNGDGKS
                     SLLGMLAGRKEPDAGRVTVRGGTTIGVLDQADTLDDALTVGGAVVGDTPEHEWAGDAK
                     VRDVIAGLVADLDWDASVASLSGGQRRRVALARLLAGDWDVIALDEPTNHLDVEAITW
                     LAGHLKTRWAGNAGALLVVTHDRWFLDEICTATWEVHDRIVEPFEGGYAAYILQRVER
                     DRMAAASEAKRQNLARKELAWLRRGAPARTSKPKFRIDAANELIADVPPIRDSVSLQS
                     LAVSRLGKDVVDLLDAGVSYPSTGSGTEEKVVLRDVEWRIAPGERTGILGVNGAGKST
                     LLGLIAGTVEATSGRVKRGKTVQVRTLTQRIDELEQHWDEPVRTVISGLRTSYTFGAG
                     SKAQDLTPGQLLERLGFSSAQLSTRVKELSGGQQRRLQLLLVLLDQPNVLILDEPTND
                     LDTDMLAAIEDLLDSWPGTLLVVSHDRYFLERVTDQQYAVMGGHLRHLPGGIDEYLRL
                     RAFEQRSGGASDGAATPAAPSKTPALDGAARRTAEKELAALERRMQKLNEQIDRAKHA
                     LADHDQSDYAGLGERMKTIQAQQDEVDELELRWFELTERLD"
     gene            11250..12254
                     /locus_tag="VN041_09380"
     CDS             11250..12254
                     /locus_tag="VN041_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29285.1"
                     /translation="MDRVRAPNIRDVARLAGVSYQTVSRVLNDSPNIRSATRDRVLAA
                     IDELGYRPNQAARTLVTSRSRIIGVLTSMSVHYGPTTAINALEVSAREAGYRISLTTL
                     ATGDDASIRGSLDFLMSQGIEALVVIAPQERIIQALADASMSIPTITLDTSGRANAHS
                     VGVDQVTGARLATQHLIDLGHTDIVHIAGPRDWIEADARMQGYLQALSDADLRTRAPI
                     LGDWSAAFGYYAGRELLRYRDFTAVFAGNDQMALGFLHACREMGIDAPGEMSVVGFDD
                     IPEAAHFAPPLTTVRQDFAEIGRRAMSMLLALIDGGDAEQARVLPELVLRASTAPAPR
                     "
     gene            12420..>12738
                     /locus_tag="VN041_09385"
     CDS             12420..>12738
                     /locus_tag="VN041_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWU29286.1"
                     /translation="MPHPSAPQDRADAAVAQVLIARTREQLCEACAAASALPSPAAVS
                     ARVDGTDLFVITPDAADLSSVGPSQTSVFGIDGRIVSAPWDRRTAPSSASSEHAGIHR
                     AGAG"
BASE COUNT         1859 a         4467 c         4416 g         1996 t
ORIGIN      
        1 ggtcgacgag gcgccccttc ccgagcgcgc cgacatcgat gatcgccggt gccgcgagcg
       61 acagccgtcc gtcccagccg agcagcgacc tccagtcggc cggcgccggc tgcggatcgg
      121 acgggatcaa cgagatcgac gcgtcgtagc cgagagcgtc gagactgccg ccgatcagcg
      181 gattgaccgc gccgtcggtc gcctcggaca gttccctgta cgcatccagc atcgccgcgg
      241 catccgatgg cagcggtgcg gaaccgccgc ccgcgagcga gctgacgacc gagtcgggcc
      301 ggaaccggga ccactcctga tcgaaccggt cgatcaccgc ggtcgcggcc ccgcgctcgt
      361 cgtccgtcag ttcgcgggag ctctcgatct cccagcgggt gccgatcgcg tcgaaccgcc
      421 acaccggcat gatgcgcctt aggccgctgc ctgctctttg atgtcctcga cggcggcgtt
      481 gaacccgccg ctcgtgagcg aggacccggc gacccgatcg accgcgagat cgtcgatcgc
      541 gacgccgacg acttcggcct cgatgccgtc gatgaactcg ccctggtact gctcggtctc
      601 cggcgcctgg gggtcgcccg tcacctcgac ctcggtgacg acgccgtccg ccagcgtcag
      661 tgtgaccgag atcgtctcga ccgtctccgg cgtctgatag gagccttcgg cggtgtacgt
      721 gccgtcggcg tactcggcgc tgctcgtcga cgcggtcgaa tccgaccccg tcatgtccgc
      781 gtcggcactg ccctcggcat ccgcggctcc ggagcagccg gcgagcacga ggagtccggc
      841 gacgccgacg acggcggcgc ccttgcgggc ggatgaggga acggtcgtgc ggatcatggc
      901 tgtcctcctg aaggtgtcgg tcgctgcgag cctagggccg cacactatgg ctgggctgtg
      961 gttcagccat gcgaaccacc gtcgacgtgg agttcatcgc cgatgccggc ctgcgccacc
     1021 gtggccgaca cgcaggcgcg accgacccta cgcgtcgccg ccgaacatgc tcgtgacgga
     1081 gccgtcctcg aacacctcgc ggatcgcgcg cgccagcagc ggcgcgatcg gaaggatcgt
     1141 gaggttctcc cagcgacgcg actccgtgag cgggatcgtg tcggtgacga cgacctcgtc
     1201 gatcgaggag tcctgcaggc gctcgttggc cgggtcgctg aagatcgcgt gggtcgctgc
     1261 gacgatgacg cggtgcgcgc cgttggcctt cagcgcctgg gctgccttga cgatcgtgcc
     1321 gccggtgtcg atcatgtcgt ccacgagcag gcaggtacgg ccctcgacgg tgccgacgat
     1381 ctcgtgcacc gagacctggt tggcgacctt cgggtcgcgg cgcttgtgga tgatcgcgag
     1441 cggagcgccg aggctgtccg accaggtgtc ggcgacgcgg acgcggccca tatcgggcga
     1501 cacgaccgtg aggatctcgc gatcctcggg ggtcagcgtg cgctcaaagt agtccagcag
     1561 cacgggcttg gcgaacaggt ggtcgacggg gccgtcgaag aagccctgga tctgcgcggc
     1621 gtgcaggtcg acgctcatga cgcggtcggc gcccgcggtc ttgagcagat cggcgacgag
     1681 acgggcactg atcggctcgc ggccgcggcc cttcttgtcc tgacgcgagt acgggtagta
     1741 cggcgcgacg acggtgatgc gcttggccga cgcgcgcttg gccgcgtcga tcatgatgag
     1801 cgtctccatg agccactcgt tgaccggctc gccgaaggtc tggatgagga acaggtcgca
     1861 gccgcggatc gacacctcga agcgcgcgta gatctcaccg gaggcgaacg tgcggtgctc
     1921 ggtcggcgcg agctcggtgc ccagcgcctc ggcgaccgcg cgcgtcagct ccggatgcga
     1981 ccggccgccg gcgacgacga gccgcttctt cgtcttggcg acgagccccg gagctatccc
     2041 gttgtcccga tccagttcaa cggtcttctt cttgcgcgcc atcgcctgcc ttttctgccg
     2101 catcgtgtgc acgggatgcc gcatcggctg cgcccgtacc cgccctgttc ttctcgaccc
     2161 acccctcgat gttgcgctga ggggcgacgc tcatggccag ggcacccgcc gggacgtcct
     2221 tgcggacgac ggcaccggct ccggtcttcg ctccagcacc cagcctaacg ggcgcgacga
     2281 gcaccgtgtg cgagccggtg tgcacctcgt cgccgatctc ggtgcggtgc ttgttcacgt
     2341 cgtcgtagtt cgcggtgatg gtgctggcgc cgaggttcac gccgcggccg atcgtcgcgt
     2401 cgccgacgta cgacaggtgc gggaccttgc tgccgtcgcc gatctgcgcg ttcttggtct
     2461 cgacgtaggc cccgatcttg ccgttggcgc ccaggaccgt gcctggacgc aggaacgaga
     2521 acggtccgac cgtggcgccg gcgccgatga ccgccaggtt ggcgtcggtg cgcttgacca
     2581 cggcatccgc accgacctcg cagtcgacca gcgtcgtgtc aggacccacg gttgcgcctt
     2641 ctgcgatcac ggtggccccg aggatctggg tgtgcggcag gatcgtgacg tcggccgcga
     2701 gcttcgcatc gtcgtcgatc caggtggtcg cggggtcgac gatcgtgacg ccggcgagct
     2761 gccagctccg cacgatgcgc tcgttgagca ggcgcccgac cagggccagc tgggcgcggt
     2821 cgttgacgcc gtaggtcacg gtgacgtcgg agacgacgga tgccgcgacg cgatcgcctg
     2881 cctggcggag gagccctggg acgtccgtga ggtacttctc gccctgcgcg ttgtcgacgc
     2941 cgacggtcgg caggtaccgg cggagctctg ctgcgcggaa cacgtacatg cccgcgttga
     3001 tctcgctcac cgcggcttcg tccgccgtgg catccttctg ctcgacgatg cggtcgacgt
     3061 cgccggcggc gtcgcggatg acgcgcccat agccggtcgg gtcttccacg accgcggtca
     3121 tgagcgtggc cgcggctccc gtggaccggt gctcgtcgac gaaagcggtg agcgtgtcga
     3181 cgtcggccag cgggcagtcg ccggacagga tcagcacgtc gccgtcgaag tccgggatcg
     3241 catccagtgc gacctcgacc gcacggccgg tgcccggcac gtcgtcctgg tcgacgaaca
     3301 cggtgccggg gtagtcctcg gcgagcgccg cgacgacctg gtcgcgctcg tggcggacca
     3361 cgacctcgat gtgctcggca cccaggcgcc gagcggtggt cagcacgtgg ccgacgaggg
     3421 ggcgcccggc gatcgggtgg agcaccttcg gcaggcgcga gcgcatccgg gtgccctgcc
     3481 ccgcggcgag gacgacgatg gcgaggttgt tctgcgtcat gctccgccgc caggactcga
     3541 acctgaacct cacagctcca aaggctgtcg tgctgccatt acaccacggc ggaccgcggc
     3601 ccccgtggga caccgcccgt caagtctgcc acgttcaccc gtgcgctctg agcgcggcaa
     3661 cggacgtcgg cccgctcgtc atctgccgga aacactcccc tgcgatcatg agggcatgag
     3721 catcgtcatc gagcgcgtcg atacggccac ccccgcgctg caggcgttcg tcgccgcgca
     3781 ccacgccgag atggagggca ccgccccgcc ggagagccag cacgcactgt ccttcgaccg
     3841 gctgctcgag ccgggcgtcc ggctcttcgc cggctaccac gagggccgtc ccgtcgccac
     3901 cggcgcgctg gctgcggtcg acggcggcca cgaggagctg aagtcgatgc gcatcgctcc
     3961 gacctcccga cggcaggggt tcggccgcgc catgctcgcc ttcctgatcg gggacgccgg
     4021 gagccggggc atccggcggc tgtcgctcga gaccggacgc gatccgttct tcgctcccgc
     4081 ccgcgccctg tacgccgacg ccggcttcgt ggagtgcgag gcgttcgggc ggtacctgcc
     4141 cgatccgcac agcgcgttcc tgactctcgc ccctgtgccg gtggcgggca tggaggcggc
     4201 gccgcacgcg ggataatggt gcgatgagcg aggtggatga ggtcgatcgg atcgtcagcg
     4261 cgtggaacgc gcagcgtccc gacctggact tctcccctct cgaggtgctc tcccgcatgg
     4321 accggctctc ccgccatctg gacagggcca ggcgcgaggt gttccaccgc agcgacatcg
     4381 agccctggga atgggacgtg ctctccgcgc tgcggcgcgc gggctcgccc ttccagctct
     4441 cgcccaagca gctgctgcag cagacgctgg tgaccagcgg caccatgacc aaccgcatcg
     4501 accggctcgt cggccgccgg ttcgtgcgcc gcgaggcgga tccaggcgac ggccgcagca
     4561 tcctggtcat cctcaccgat gacgggaggg tgcgcgtgga tgccgccatc acccgcctcg
     4621 tggatgccga ggccgagctg ctgcaggcgc tgccccgcgc ggaccgcgac cggctggcgg
     4681 gtctcctgcg caagctcagt ctgagcttcg acgtatgagc gcgcgctgat ggcgatgacc
     4741 tcaccgctgc cggtgcgcga cggcgtcggt gcgacccggc tgcacctgcc gctgagcggc
     4801 ccgtggccca cgatcggcgc gtacatggcc gagcgcttct tccacctcga cccggaacga
     4861 ctgctgacgc gcttcgaccg cggcgagatc gtggcgcgag acggctcgcc cctcgccagg
     4921 gacaccccgc tgggcgagca cgagttcgtc tggtactacc gggagccgcc ggtggagcgc
     4981 gagatcccgt tcgagatcga ggtgctgcac caggaccgcg acctcgtggt ggccgacaag
     5041 ccgcacttcc tgccgaccac cccgggcggc aagtacctgc agaactccgc cctggtgcgg
     5101 ctgcggaacc tgctcgggat ccccgagctg accccgatcc atcgtctgga ccgggcgacc
     5161 gcgggcctgc tgatgttctc ggtgcgcccg gagacccggg gcgcctacca gctgctgttc
     5221 gagaaccgcc gggtggagaa ggtgtacgag gcggtgtcgg ccctgcccga ggggtgggat
     5281 gcggcatccc cggcgctcga cggacgtccg ttcccgatcg tccaccgcaa ccacatcgag
     5341 aagctccgcg cgaacgtgtg cgtacgggtc gacggcgatc gcgagcccaa ctccgagacg
     5401 ctgatcgagt tgatcgacgc ggacgagagc gtgctgcaca cgcgactccg gccgcactcc
     5461 gggaagatgc accagctgcg ggtgcacctg gcggcgctgg gcgccggcat cctcaacgac
     5521 ggcttctacc ccgtgctgct gcccgaagcg cccgacgact tcacgaagcc cctgcagctg
     5581 ctggcccgcg aactgcgatt cacggatccg ctcaccggcg ccgcacggga gttcgtgacg
     5641 cgcaggaccc tgcaggaggc gcctaccggc gcatgatcac gcgcgcgagc ttcgtcccca
     5701 gcagctgcac gaggtgcacg aacacgacga tcagcacgat ggccgcccac atcacgaccg
     5761 gttcgaactg acggaagccg tagatctgcg cgaaggcgcc cagaccaccg ccgcccacga
     5821 ggccggccat ggcggtcatg tcgatgaggg ccaccacgat gaacgtgtag ccgaggatga
     5881 gcggcccgag cgactcgggg atcgcgacgg tcaagaggat gcgccacggg ccggcgccca
     5941 tcgcgcgcgc ggcctcgagc acgcccggtg agaccgacac gaggtgctgc tcgacgatgc
     6001 gcgcgatcgc gaaggaggcc gcgagaccga tcgcgaaggc gccggccgtg gtgccgatgc
     6061 cgacgccgat cacggcccgg gtcagcggca tcgccacggc gatgaagatc acgaacggga
     6121 tcgggcggaa gaagttcacc agcagctcga gcagcccgtt gacgaagcgg ttcggcagca
     6181 gtccgccacg gcgggtgagg tacaggccca cgccgatgag ggtgccgaga acgcccgcca
     6241 tgaccagcgc gaacaccgtc atgtacagcg tctcgacggc ggccttccag aactcgtcgc
     6301 ccagttcgat gagacgatcc atcagcgcgt cccctcctgc tcgagctcgg tcacttcagc
     6361 gtgctgcctg atgccgcgca gcgcctcgtc gatcgtggca tccgcacccc ggatcgccag
     6421 ggtgagatgg ccgaaggcgc gaccgcggat gtcgttgatg ccgccgtaca cgagctcgaa
     6481 ctcgaggccg gcctgtgcga ggtccagcag cacctgcgac tgcgaggagt ccccatcgcg
     6541 gaacgagaac gtgaccagac gtccggcgtg ccgctcacgg agagccgcag cctcggccgg
     6601 cgagggcagt cccttgatga ccgtgccgac gaagcgctgg gaggcggggt tctgcggtcg
     6661 ggagaagacg tcgaagacct cgccctgttc gatcacccgg cccttctcca tcacggcgac
     6721 cttcgtcgcg atcgtctgga tgacgtccat ctcgtgcgtg atgacgacga tcgtcacgcc
     6781 ctgctcgcgg ttgacccgcc ccagcagatc gagcacctcg tgcgtggtct gcgggtcgag
     6841 cgcgctggtc gcctcgtcgg cgagcaggat gctgggccgg gtcgcgagcg cacgggcgat
     6901 gcccacgcgc tgtttctggc cgccggagag ctgctcgggg taggcacgcg ccttgtcggc
     6961 gaggcccacg aatctcagca gctcggccac gcgctcgtcg atctcgcgcg gggaccagcc
     7021 ggcgagcttc agcggatagg cgatgttggc cttgacggtc ttcgcggaga acaggttgaa
     7081 ctgctggaag atcatgccga tcccgccgcg gatccggcgc agctcccgct cgcccagcgc
     7141 ggtgatgtcg acgccgtcga cgtggatcgt cccgctcgtg gcgggttcga gggcgttgat
     7201 cagccgcacc agagtggact tgcccgctcc ggagtagccg atgatgccga acacgtcgcc
     7261 ctgctcgatg tcgagggtga cgtcgtcgac tgcggtcaca ggtgcgctgt cgcgccccgc
     7321 tgccgggtag ctcttggtga cgccggcgag gctcacgatg ggcatgtgtt ctcccgaggg
     7381 tggggatgcg gtgtgcacgg aatcgggcgg cgcgagttcg cgccgcccga ttctctcgca
     7441 ttcaggtgtg aacgcgggat atcacttctt gatgacggtg tccttgagaa ccgtcttcag
     7501 cgacttctgc aggtctgcgg ccgcggtctt cagcggcacc gccgtgtcgc ccgaggactc
     7561 ctgcaggccc ttctgcaccg ccgcgtcggt ctggaagatc tcgacgagct tcttgtaggt
     7621 cgggttgtcg acgtcatcgg cgcgggccgc gaagatgttc acgtacggca acgcgttcgg
     7681 gtcggtcggg tcgtcctgcg cgatggcgtc cttgaagctc aggcccgcat ccttgacgaa
     7741 gtcgttgttg atgatcgccg cggcgacgtc gggcagagcg gtcgggatga cggctgcctc
     7801 gagcgcggag accttgacct tggacttgtc ggtgtcgacg tcggcgaggt cgctgacgat
     7861 ggtgcctccg ctcttcaact tgatgaggcc ggccgactgg agcaccagca gtgcgcgggc
     7921 ctggttggag gcgtcgtccg gcacggcgac ggtctcgccc ttcgggatgt ccttcaccga
     7981 gtcgtacttc ttcgagtaca ggcccagcgg gtagatcgcg gtcgcgccga tcggctgcag
     8041 gtccttgccc gaggccttgt cgtactgcgc gaggtagacg atgtgctgga actggttcag
     8101 gtcgagctcg ccctcggcga gcgcggggtt gggctgctcg taggagccga agtcgaccag
     8161 ttcgaccgag atgccctcct tcttggccgc ggcgacgaac gcgggccact gcgcgtcgct
     8221 cttgccgacg acgccgatct tgaccgtgtc gtccttcttg gcggagtcgg tggaggatgc
     8281 gggattggag gcgcacccga cgagggaggc gatcaggaca gcggccgttg cggcggctgc
     8341 tgcgagggtg gtgatgcgac gggacatggt ctgtgattcc tctcgtgggg gactcgaaaa
     8401 cggtacgtgg cgcctccggg cgcaccgtaa tcggctcgtc acattacgtc acagcaccgg
     8461 cggcctcact acgcttcgcg cggcgcccgg acggagaggg gcatcaggcc cgtgggagtg
     8521 gacgaaccac ctcaccacgg gcctggtcgc gtccccagcc gcgagagcag cgccgcggtc
     8581 ccctcgatcc cccaaccgag gtagacgggc gtctatatgc tcgtagacga gcgtatatca
     8641 ctcgggtgat gaggtccaga ccgcccggat gccgtcgacg acgacggatt cggcgtcccg
     8701 gacaccgagt ccggtcaggg ccgtggcgaa gctgtcgacg gcgttctgcg ccgagtagtc
     8761 cgtgaggaac cgcgcgatcg cgagttcccc ggacacgagc agctgcagga acagcgtggc
     8821 ctgcgccggc ggcatccgca gctccccgcc ctcacgggcg aggtgatcgg cgaggaagcc
     8881 cgcgacgacg tcgcgcgagg tgacgtagcc gcgccgcgcg atctcgacgg gcacggagcg
     8941 gtcctgatag agcgcgagcc atgcagcggc gatcgggtcg gccgctcccg cccgcgcgag
     9001 cccggcgaag tacgcgatgc agccacgcat cgcactgtcg gggaaaagcc ggcgcgcgtc
     9061 cgccgcgacc tggacggcga tctcgtccgc gcgatcgaac accgcgatcg cgatgtcctg
     9121 cttggtcggg aaatggtgcc cgagcgcgcc cttcgtgagc ccgagcctgg cggcgatccg
     9181 cgacagcgat gcggcggcgt agccgcattc gatcatctcc tcgctggcgg cggtcagcag
     9241 gcgcagtcga gtgcggtgtg cgggggaatc ggggagcacc catccaccat agggcgcgag
     9301 agcgccgcgg tgcaggtcag tccagccgct cggtgagctc gaaccagcgc agctcgagct
     9361 cgtccacctc gtcctgctgc gcctggatcg tcttcatccg ctcgccgagc ccggcatagt
     9421 cggactgatc gtggtcggcg agcgcgtgct tggcgcgatc gatctgctcg ttgagcttct
     9481 gcatcctgcg ctcgagggct gcgagctcct tctcggccgt gcgacgggcc gcgccgtcca
     9541 gcgccggggt cttcgagggc gccgccgggg tcgcagcacc gtcggacgcg ccgccggacc
     9601 tctgctcgaa agcgcgcagg cggaggtact cgtcgatacc gcccggcagg tgccgcaggt
     9661 ggccgcccat cacggcgtac tgctggtcgg tgacccgctc caggaagtac cggtcgtggc
     9721 tcacgacgag cagcgtgccc ggccacgagt cgagcagatc ctcgatcgcg gcgagcatgt
     9781 cggtgtccag gtcgttcgtg ggctcgtcga ggatcagcac attcggctgg tccagcagca
     9841 cgagcagcag ctgcagccgc cgctgctggc cgcccgagag ctccttcacg cgcgtggaca
     9901 gctgcgcgga gctgaagccg agccgctcca ggagctgacc gggcgtgagg tcctgcgcct
     9961 tcgacccggc cccgaaggtg tacgaggtgc gcagcccgct gatgaccgtg cgcaccggct
    10021 cgtcccagtg ctgctcgagc tcgtcgatcc gctgggtgag cgtgcgcacc tgcacggtct
    10081 tgccgcgctt tacccgcccg ctcgtggcct ccacggttcc ggcgatcagc ccgagcagcg
    10141 tggactttcc cgcgccgttg acgcccagga tgccggtgcg ctcccccgga gcgatgcgcc
    10201 actcgacgtc gcgcagcacc actttctcct cggtccccga gcccgtcgaa gggtacgaga
    10261 cgccggcgtc cagcagatcg acgacgtcct tgccgaggcg ggagaccgcc agcgactgca
    10321 gcgagacgga gtcgcggatc ggcggcacgt cggcgatcag ctcgttggcc gcgtcgatgc
    10381 ggaacttcgg cttgctggtg cgcgccggcg caccgcgtcg cagccacgcc agctccttgc
    10441 gcgccaggtt ctgccgcttc gcctcggatg ccgcggccat ccggtcgcgc tcgacgcgct
    10501 gcaggatgta ggccgcgtac ccgccttcga acggctcgac gatccggtcg tgcacctccc
    10561 acgtggcggt gcagatctcg tccaggaacc agcggtcgtg cgtgaccacc agcagcgccc
    10621 ccgcattccc tgcccaccgg gtcttcaggt gcccggcgag ccaggtgatg gcctcgacgt
    10681 cgaggtggtt ggtgggctcg tcgagagcga tgacgtccca gtcgcccgcg agcagccggg
    10741 ccagcgcgac ccggcgtcgc tgccctccgg agagggatgc caccgaggcg tcccagtcga
    10801 ggtcggcgac gaggccggcg atgacgtcgc gcaccttcgc gtcgcccgcc cactcgtgct
    10861 ccggggtgtc ccccacgacc gctcccccga cggtgagggc gtcatcgagg gtgtccgcct
    10921 gatcgagcac gccgatcgtc gtccccccgc gcacggtcac gcggccggca tccggttcct
    10981 tcctcccggc gagcatcccg agcaggctcg acttgccgtc gccgttgcgc ccgacgatgc
    11041 cgatgcggtc cccctcctcg atgccgaggg tgaccgagtc gaagacgacg cgcgtggggt
    11101 actcgaggtg gagcgcttcg gcgccgagaa gatgtgccat gtcgtcttcc agggtagtcg
    11161 tgcgggcctg tcgtgctttg cggccttcca ggccgcctcc ataatggagt gcaccatgtg
    11221 aacgtacaca tcgagggggt cgaggcactg tggaccgcgt ccgggcaccg aacatccgcg
    11281 atgtcgcgcg cctggccgga gtctcgtacc agaccgtctc ccgggtgctg aacgactccc
    11341 ccaacatccg ctcggcgacc agggatcgcg tgctggccgc gatcgacgag ctcggctacc
    11401 gcccgaacca ggccgcgcgc acgctcgtga ccagccgatc ccgcatcatc ggcgtgctca
    11461 cctcgatgtc ggtgcactac ggcccgacca ccgcgatcaa cgcgctcgag gtctcggccc
    11521 gcgaggccgg gtaccgcatc agcctgacca cgctggccac gggcgacgac gcctcgatcc
    11581 gcgggagcct ggacttcctg atgtcgcagg ggatcgaggc gctcgtcgtg atcgcacccc
    11641 aggagcgcat catccaggcc ctcgcggacg cgtcgatgag catccccacg atcacgctcg
    11701 acaccagcgg tcgcgcgaac gcgcacagcg tcggcgtcga tcaggtcacg ggcgcccgcc
    11761 tggccaccca gcacctgatc gacctcggcc acaccgacat cgtgcatatc gccgggcccc
    11821 gcgactggat cgaggcggat gccaggatgc agggctacct gcaggccctc tcggatgccg
    11881 accttcgcac ccgggcgccc atcctcggcg actggagcgc ggccttcggg tactacgcgg
    11941 gtcgcgagct gctgcgctac cgggacttca cggcggtctt cgcgggcaac gaccagatgg
    12001 cactcgggtt cctgcacgcc tgccgcgaga tgggcatcga cgctccgggc gagatgagcg
    12061 tggtcggctt cgacgacatc cccgaggccg cgcacttcgc cccgccgctc accaccgtgc
    12121 gacaggactt cgccgagatc ggccgccgcg ccatgtcgat gctgctcgcc ctcatcgacg
    12181 gcggggacgc cgagcaggcg cgggtcctcc ccgagctcgt gctgcgcgcc tcgacggcac
    12241 ccgcgccgcg ctgacccggg cccggacggg cgtcgatcat cacgaatcgg taaccgctcc
    12301 acgtcgggcc gttgacagat gtgaccgttt cgcatgtatc atcgagcaca cgttgtgaac
    12361 ggtcacaaat gagagcacgg cccgacagag gccacccaca agacgatgga gtcccgaaga
    12421 tgccgcaccc ctcagccccg caggatcggg ctgacgccgc cgtggcgcag gtgctcatcg
    12481 cccgcacccg cgaacagctc tgcgaggcgt gtgccgcggc atccgccctg ccctcccccg
    12541 ccgcggtgtc cgcacgcgtc gacggcacgg acctgttcgt gatcacgccc gatgccgccg
    12601 acctgtcctc cgtcggcccg tcgcagacct ccgtcttcgg catcgacggc cgcatcgtga
    12661 gcgcgccgtg ggaccgccgc acagctccct cctccgcatc ctccgagcac gccggcatcc
    12721 acagggcggg cgcaggac
//
LOCUS       DATMSX010000308         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_42784,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000308 DATMSX010000000
VERSION     DATMSX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42784"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN041_09390"
     CDS             <1..349
                     /locus_tag="VN041_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29287.1"
                     /translation="LLRDHTELHDVVRRVEAADAIIAFRQRASLPKGLSAETLERVSA
                     ALAQHPDASAAELGEVLSISRVSARRYLEHLASTGRALRSLDYSTKGRPGTRYRANEP
                     VGTLGTVSQIVRD"
     gene            403..>1512
                     /locus_tag="VN041_09395"
     CDS             403..>1512
                     /locus_tag="VN041_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU29288.1"
                     /translation="MFRSTYPDVEIPAISIYDYLFGDLDEATLDRVAIVDGPTGAETT
                     YRQLIGQIDLFAGALAARGAGVGTTVAVLCPNVPAFATVFHGILRAGATATTLNSLYT
                     ADEIENQLRDAEAEWLVTVSPLLAGAKEAAARAGFADDHVIVLDGAEGYPSLRDLLGE
                     GRPAPEVSFDPATHLAVLPYSSGTTGRPKGVMLTHRNLVANVAQCQPPVISVDSSDRL
                     LAVLPFFHIYGMTVLLNFALRQRAGLVTMPKFDLPEFLRIIAEHRTTWVFVAPPIAVA
                     LAKHPIVDQYDLSSVKVIFSGAAPLDGALASAVSERLHCRVVQGYGMTETSPAVNIVP
                     ADRTDIDPSSIGPLVPNTEARLVDPDTGADVEPPAA"
BASE COUNT          220 a          553 c          505 g          234 t
ORIGIN      
        1 gctgctgcgc gaccacaccg agctgcacga cgtggtgcgc cgggtggaag cggcggacgc
       61 catcatcgcg ttccggcagc gcgcgagtct gcccaagggg ctcagcgcgg agacgctgga
      121 gcgggtctcg gccgcgctgg ctcagcatcc ggacgcctcc gccgcggagc tcggcgaggt
      181 gctgtcgatc tcacgcgtga gcgcgcgacg ctacttggag cacctggcca gcacggggcg
      241 agccctgcgc tcgctggact actccaccaa gggcagaccg ggaacgcgct accgtgcgaa
      301 cgagccggtc ggaacccttg ggactgtgtc tcagattgtg cgagactgac tcccataact
      361 gggcggtgtg cctgacgccg cacgtcgatc aaaggggatc ccatgttccg cagcacgtac
      421 cctgatgtcg agatccccgc gatctcgatc tacgactacc tcttcgggga tctggacgag
      481 gccacgctgg accgcgtcgc catcgtcgac gggcctaccg gcgcggagac gacctatcgt
      541 cagctcatcg gccagatcga cctcttcgcg ggtgcgcttg cagcccgcgg tgccggcgtc
      601 ggcaccacgg tggccgtgct ctgccccaat gtcccggcgt tcgcgaccgt gttccacggc
      661 atcctgcggg cgggcgcgac ggccaccacc ctgaactcgc tgtacaccgc cgacgagatc
      721 gagaaccaac tgcgggatgc cgaggcggag tggctcgtga ccgtgtcgcc gctgctcgcc
      781 ggcgcgaagg aggcggcagc gcgcgccggc ttcgccgacg accacgtgat cgtgctcgac
      841 ggcgcggagg gataccccag cctgcgcgat ctgctgggcg aggggcgccc cgctccggag
      901 gtgtcgttcg atccggccac gcacctcgcg gtcctcccct actcctccgg caccaccggg
      961 cgcccgaagg gcgtgatgct cacccaccgg aacctggtcg ccaacgtggc gcagtgccag
     1021 ccgccggtga tcagcgtcga ctcgtcggac cggctgctgg cggtgctgcc gttcttccac
     1081 atctacggca tgacggtgct gctcaacttc gcgctgcgtc agcgtgcggg cctggtgacg
     1141 atgccgaagt tcgacctgcc ggagttcctg cgcatcatcg ccgagcaccg caccacctgg
     1201 gtgttcgtgg cacccccgat cgcggtcgcg ctggccaagc atccgatcgt cgaccagtac
     1261 gacctgtcct cggtcaaggt catcttctcc ggcgcggcac ccctggacgg cgcgctcgcg
     1321 tcggccgtgt ccgagcggct gcactgccgc gtggtgcagg gctacggcat gaccgagacc
     1381 agcccggccg tgaacatcgt gcccgccgac cgcaccgaca tcgatccctc gtcgatcggt
     1441 ccgctcgtcc cgaacaccga ggcccggctc gtcgaccccg acaccggtgc ggacgtcgag
     1501 ccgccggccg cc
//
LOCUS       DATMSX010000309         1947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156458,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000309 DATMSX010000000
VERSION     DATMSX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156458"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VN041_09400"
     CDS             <1..427
                     /locus_tag="VN041_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29289.1"
                     /translation="LAIATAVLAAACVVALIALHLARWTQLTRLPGVLRGSPTEVEQE
                     RGRSLTRDLRGQMLATMTLWSLAAFASMGLVLGEDVVATSSGRTLGLLGILVLWVAGL
                     SLLTWLRVRLERLGPLLREEFGYDVPVRTASGDSSPGLV"
     gene            complement(373..987)
                     /locus_tag="VN041_09405"
     CDS             complement(373..987)
                     /locus_tag="VN041_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29290.1"
                     /translation="MARRGSYAKGIARREEILDSALEVIGRDGYQNASLRGIAAEVGV
                     TPAALLHYFGTKEELFTAVLRRRDERDGLRAEDGSDDARAGFIRAIRHNTEVPGLVEL
                     FSRLAVDAVDPEHPAHDYFLERNARLRALMSAAVPSASGAALDPETLARVIQAVSDGL
                     QLQWMIEPEVDMAGIIDRLITVLERAGQTSPGDESPDAVRTGTS"
     gene            1094..>1947
                     /locus_tag="VN041_09410"
     CDS             1094..>1947
                     /locus_tag="VN041_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU29291.1"
                     /translation="MTEASDLTLEEKASLTSGADFWTTKAIERLGVPSIMVTDGPHGL
                     RKQAGASDHLGIADSVPATCFPPAVGIGSSFDPELIERVGAAIGVEAAIEDVAVVLGP
                     GINIKRSPLCGRNFEYFSEDPIVSGVLGAASVRGIQSQGVGTSLKHFAANNQEHDRMR
                     SSSDVDPRPLREIYLRGFERVVKDAAPWTVMCSYNKLNGVWTSEDPWLLTSVLRDEWG
                     FDGLVVSDWGAVNDRIAGIAAGLDLEMPASGGRTDAQLVAAVQAGELDEKVLDVAASR
                     ALQLVRRAG"
BASE COUNT          273 a          670 c          690 g          314 t
ORIGIN      
        1 gctcgccatc gccaccgcgg tgctcgcggc cgcatgcgtg gtcgcgctga tcgcgctgca
       61 tctcgcccgc tggacgcagc tcactcgtct gccgggcgtg ctccgcgggt cgccgaccga
      121 ggtcgagcag gagcgcggac gttcgctcac gcgggacctg cgcggccaga tgctggcgac
      181 catgaccctg tggtcgctcg cggccttcgc gagcatgggg ctcgtcctgg gcgaggacgt
      241 ggtcgccacg agttccggaa gaaccctcgg cctcctggga atcctcgtcc tgtgggtcgc
      301 ggggctgtcg ctgctcacct ggctgcgggt gcggctggag cgcctcggtc cgctcctgcg
      361 cgaggagttc ggctatgacg tgccggtgcg cacggcatcc ggtgactcgt cgccagggct
      421 tgtctgacct gctcgttcga gcacggtgat cagccggtcg atgatgccgg ccatatcgac
      481 ctcgggctcg atcatccact gcagctgcag cccgtcggag acggcctgga tgacgcgcgc
      541 gagcgtctcg gggtcgaggg ccgcgccgga tgcggagggg acggcagcgg acatgagcgc
      601 acgcagccgt gcgttgcgct cgaggaagta gtcgtgcgcc ggatgctcgg ggtcgacggc
      661 gtccacggcg agccgggaga acagctcgac cagtcccggc acctcggtgt tgtgccggat
      721 ggcgcggatg aagcccgcgc gggcgtcgtc gctgccgtcc tcggcgcgga ggccgtcgcg
      781 ctcgtcgcgt cggcgcagca cggcggtgaa cagctcctcc ttggtgccga agtagtgcag
      841 cagcgcggcc ggggtcacgc cgacctcggc ggcgatgcct cgcagcgacg cgttctggta
      901 gccgtctcgg ccgatcacct cgagggcgct gtcgaggatc tcctcacggc gggcgatgcc
      961 cttcgcgtac gaacctctgc gtgccacgcc ccgagcctaa tcctgaacag tggttgattt
     1021 ccagaaattg aaggtcgtat agttttcgca tgacagcgac gtcgccatgg acgtccccat
     1081 ggaaggaacc ggcatgaccg aagcatccga tctcactctc gaggagaagg cctcgctcac
     1141 cagcggtgcg gacttctgga ccaccaaggc gatcgaacgc ctcggcgtgc catcgatcat
     1201 ggtcaccgac ggcccgcacg gcctgcgcaa gcaggccggc gccagcgacc acctcggcat
     1261 cgccgacagc gtgcccgcca cctgcttccc gcccgccgtc ggcatcggct cgtccttcga
     1321 cccggagctg atcgagcgcg tgggcgcggc gatcggcgtc gaggccgcga tcgaggacgt
     1381 cgcggtcgtg ctcggccccg gcatcaacat caagcgctcg ccgctgtgcg gccgcaactt
     1441 cgagtacttc tccgaggacc cgatcgtctc gggcgtgctg ggcgcggcat ccgtccgcgg
     1501 catccagtcg cagggcgtgg gcacctcgct caagcacttc gcggccaaca accaggagca
     1561 cgaccggatg cgctcctcga gcgacgtcga tccgcgtccg ctgcgcgaga tctacctgcg
     1621 cggcttcgag cgggtcgtca aggatgccgc tccctggacc gtgatgtgct cgtacaacaa
     1681 gctcaacggc gtgtggacca gcgaggaccc gtggctgctg accagcgtgc tccgcgacga
     1741 gtggggcttc gacgggctgg tcgtctcgga ctggggagcc gtcaacgacc gcatcgccgg
     1801 catcgccgcc ggtctggatc tggagatgcc cgcctcgggc gggcggacgg atgcccagct
     1861 cgtcgccgct gtgcaggccg gtgagctcga cgagaaggtg ctcgacgtcg cggcgtcgcg
     1921 cgcgctgcaa ctggtgcgcc gcgccgg
//
LOCUS       DATMSX010000310        11513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_14152,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000310 DATMSX010000000
VERSION     DATMSX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14152"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN041_09415"
     CDS             <1..219
                     /locus_tag="VN041_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29292.1"
                     /translation="LRARIWGEPDEDLRGLPRGVAFAAHAFGFISGAEQVRVTRSGRW
                     TRLAFARGHVLSRGPAVTGLTAVRETGH"
     gene            complement(275..1933)
                     /locus_tag="VN041_09420"
     CDS             complement(275..1933)
                     /locus_tag="VN041_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HWU29293.1"
                     /translation="MASRTHLTDVLVIGWGLSGLVAAAEALEAGRRVTVIDQEPRSNL
                     GGQAWWSFGGLFLVDSPEQRRMGIRDSLELATQDWFGNAGFDRPEDEWPRRWASAYLE
                     FAAGEKRSWLRERGVGFFPVVGWAERGGGGAIGPGNSVPRFHITWGTGPGIVAPFAAA
                     MERAEAEGRATILPRHRVTRLIVEDGTVAGASGDVLADELTARGVPSSRERSGEFEIR
                     ADAVIVASGGIGGNHDLVRAAWPDRLGTPPEHMLTGVPAYVDGSMHAVSEEAGAHLIN
                     GDRMWHYVEGIENWDPVWPSHGIRILPGPSSLWLDATGTRLPVPLYPGFDTLGTLAHL
                     RTTGHDHSWFITSLKIVEKEFALSGSEQNPDLTGKDVGLLVKSRLAKGPTGPVQSFLD
                     EGADFVVENDLESLLTQMKRMPGGESLDVDRARQEVLARDREIGNDFTKDGQIAMLRS
                     MRAYRGDRLIRTAPPHRLQDPAAGPMVAVKLHVLTRKSLGGIQTDLDGRALAADGAPI
                     PGLFAAGEASGFGGGGVHGYRALEGTFLGGCLFSGRQAGRAASR"
     gene            complement(1964..2407)
                     /locus_tag="VN041_09425"
     CDS             complement(1964..2407)
                     /locus_tag="VN041_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HWU29294.1"
                     /translation="MTFYDEIGGHATFQRIVEVFYREVAVDPVMRPMYPEDDLGPATE
                     RLTMFLEQYWGGPGTYSERRGHPRLRMRHVPFHVNPDARDRWLAHMRTALDEAQLSPL
                     HDATFWDYLQRAAHSLVNTFEDSPEPPPTTIGPTQERRPDLGLTS"
     gene            complement(2404..3384)
                     /locus_tag="VN041_09430"
     CDS             complement(2404..3384)
                     /locus_tag="VN041_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWU29295.1"
                     /translation="MNAIWVAVIIASCVVAAFVLRLVIRRIVKRIVDSAKSKARVDDT
                     QALERSPLADMRLVQRTRTLGTILQNIVNVILVVVAIVLTVNQLSPSLLGSLTLLTAA
                     VGAGLGFGAQNIVKDVLNGLFLVAEDQIGIGDVVDLGLASGVVEYVSVRITQVRDVNG
                     TLWYVRNGEVTRIGNMSQGWARAIIDLGVQPDADLELVESTMLETAQGLAKDPKWRTR
                     IIERPEVWGLESVTGDALVVRVVMKTRANAMDDVAQELRARLRQALTEKDVLVPSLAS
                     VTLSGLEGARRVRGANPPKTRPNAITGVPPVAERGVWRRKKTGDKEGDAK"
     gene            complement(3553..6123)
                     /gene="pepN"
                     /locus_tag="VN041_09435"
     CDS             complement(3553..6123)
                     /gene="pepN"
                     /locus_tag="VN041_09435"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWU29296.1"
                     /translation="MPGENLTRTEAQERRAVIDTQSYQVSLDLTKGAEVFGSRSVVRF
                     TATPGGATFIDLIAKEVREISLNGEQIDPSEAFADSRIALSGLQAENVLIVDADCLYT
                     NSGEGLHRFVDPVDGEVYLYSQFEVPDSRRMFAVFEQPDLKATFQFTVTAPAAWKVVS
                     NSPTPEPIVHDASTDSGTQQTATWGFEPTPRISSYITALIAGPYEVTFSELTSASGRV
                     IPLGVYGRKSLWAHLDADYIFDKTREGFAYFESKFGVPYPFAKYDQLFVPEFNAGAME
                     NAGAVTFTETYVFRSKVTDAVKERRVVTILHELAHMWFGDLVTMKWWNDLWLNESFAE
                     WASTIATAEATEWTEAWTTFAAMEKTWAYRQDQLPSTHPIVAEINDLEDVQVNFDGIT
                     YAKGGSVLKQLAAWVGIEAFFAGVSQYFQKHAWGNTELSDLLTELEATSGRELSTWAK
                     KWLETAGVNTLAPVIAEDAAGVITRFAVTQTAPADYPTIRPHRLGIGFYNLDGEALVR
                     THYVEVDIDGDRTEIPELQSLQRPDMVLLNDKDLAYAKIRLDDRSLATAVEHLSDISD
                     PLARSLVWGAAWDQTRDAESPASAYIDLVLGNIGRETESTTVRTTLSQLLLAASSYVT
                     PENRTAAREKVADGLWALAQQAEPGSDSQLQLVTAFANVLVTPEHAGIVGRLRSGEET
                     LPGLDIDADLSWQLLNGLAAIGATDAAAIDAALAADNTSKGAEFAAQARAALPTAEAK
                     QAAWSSLIDNADLPNTIVRSAALGFVHPSGVGVLGDFIPKYFDMLVPIWEGRTYQIAD
                     YLVVGLYPRSLASVELRDATRAWLSSHQDASPALRRLVHENLADVERALAAQSRDAED
                     "
     gene            6267..7451
                     /locus_tag="VN041_09440"
     CDS             6267..7451
                     /locus_tag="VN041_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWU29297.1"
                     /translation="MISEATDVVLHASSAASGGEPFIEQPVAYDAILLAGFGGPEGQD
                     EVIPFLRNVTRGRGIPDERLEEVAHHYRHFGGVSPINGQNRALKAALEAELEARSIDL
                     PVYWGNRNWAPYLEDAVAEAAGHGDTTLLAFATSAYSSFSSCRQYREDFARVLDGTEY
                     DGVVTIDKIRPFYDHPGFVQAFVEGVHDAVAAFLAEGIPADAVQVLFSTHSIPTADAE
                     RSGPRDIDFGPGGAYAAQHESVAAWVMDRVAQLEPAAADVPWELVYQSRSGPASQPWL
                     EPDVCDVIGELAERGRQAVAVVPVGFMSDHMEVLWDLDTEAKEAAEQAGLRFTRTPTP
                     GVSPAFVAGIVDLIQERLEGRPNADRARVASVGGSFDVCRPGCCENVRAGFKPAAAGI
                     LP"
     gene            7483..7968
                     /locus_tag="VN041_09445"
     CDS             7483..7968
                     /locus_tag="VN041_09445"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HWU29298.1"
                     /translation="MRIHIATDHAGLDFSTRLQEHLRSAGHDVTDHGPVEYDALDDYP
                     AFCIRAAQAVIADQTAGVEALGVVFGGSGNGEQIAANKVQGIRAALVWNTSTAELARE
                     HNDANVISIGARQHTFDEVTSFIDTFIGTPFSGEERHVRRIAQIADFEISGSLLPDPR
                     A"
     gene            7970..8968
                     /locus_tag="VN041_09450"
     CDS             7970..8968
                     /locus_tag="VN041_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWU29299.1"
                     /translation="MPEGHSVHRIARQFARNFVGLPVHASSPQGRFAEGAAVLEGREM
                     LQAMAVGKQMFLEFEGDTWLRVHLGLYGAWDFAGEILVDPTIASANGRMGQTNQRGTD
                     LDGVILDDAGENSLTSIGAPRKTRVHVRMSEQTKGLADEDAEWPPPVVGQVRLRLLTD
                     ATCADLRGPTACVLQTPDEMLATIAKLGPDPLVGDPAEGEERFVRIVRRKPTAIALLL
                     MDQAVVSGIGNVYRAEMLFRARLNPHTPGRDVPEEVVRQLWRDWVRLLAIGVETGQMM
                     TMDDLSPEQYRAAMASRDDRHWVYRRAGLPCRVCGTEIALEELGARKLYWCPFCQR"
     gene            9059..10534
                     /locus_tag="VN041_09455"
     CDS             9059..10534
                     /locus_tag="VN041_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HWU29300.1"
                     /translation="MDLSGLQAVLENVSSWIWGPWVLIPLLLGTGLYLTIRLGGIQFL
                     KLGAALRLGLFTRKDPDSDGDISQFQALTTALAATVGTGNIVGVATAIGIGGPGALFW
                     MWVTGLLGMASKYSEAFLGVRFRTTDAAGEKSGGPQYYLERGIKNGFGKFLAIFFAIS
                     AVIACFGIGNMTQGNSISANLENSFNVPTWVTGLVLTVFALLVLVGGIKSIGRVTAGL
                     VPVMIIFYVLGAIYILIANIAGVPAAFAQIFSEAFTGTSAVGGFAGSLIIVAVQMGVA
                     RGIFSNESGMGSAAIAAAAAKTSHPVRQGLVSMTQTFIDTIIVVTCTGLVIITTGTWN
                     AVDDEGKQISPALMTGEAFSHGLPGEWGHYIVTIGLVLFAGSTILGWSYYGERSLERL
                     VGVKAIMPYRIIFSLVVFVGCTTELGVVWAFSDVMNGLMALPNLIGLLVLSGLVARET
                     KKYLTNDPKLTATTDEVNAFMAGDAGFEDWKTQAVPVVKAR"
     gene            10594..11235
                     /locus_tag="VN041_09460"
     CDS             10594..11235
                     /locus_tag="VN041_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWU29301.1"
                     /translation="MRQNPSFAMTDIAELRRVISQSPWATLVATGDDGLVASHYPVLL
                     DPERDDLTVVGHVGKPDDQVLHLGERELLMIFQGPHGYISPSWYGDVRAVPTWNYTAV
                     HLTGIPEVVDTQANLRILDDLVTHFEQRLPQPRMMWQAPNTEEFVNALEMGTVGFRMT
                     PTRVSAKRKLSQNKPDSIVENVIAELDGSGEYAHPALAAEMRRAHEARLAHRG"
     gene            11232..>11513
                     /locus_tag="VN041_09465"
     CDS             11232..>11513
                     /locus_tag="VN041_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWU29302.1"
                     /translation="MSADLLIRSVRVAGAGRELLPFASPDGDEPVDILVSGGVIADIA
                     PAGNLRATADALDGDGTWVVPGLWDHHVHTVQWALDAQRVPLGGATSAAE"
BASE COUNT         1742 a         3893 c         4042 g         1836 t
ORIGIN      
        1 ctccgcgccc gcatctgggg tgagcccgac gaggacctgc gcgggctgcc gcgtggtgtc
       61 gccttcgccg cgcacgcctt cgggttcatc agcggcgccg agcaggtgcg cgtcacgcgg
      121 tccgggcgat ggactcgtct cgctttcgcg cgggggcacg tgctcagtcg cggtccggcg
      181 gtgaccggac tcacggcggt gcgggagacc ggtcactgaa tcgaccgggg atgctgaacc
      241 cgttgaacgc ggccggcgat cccgagcaag caggtcagcg gctcgctgcg cgtcccgcct
      301 gacgcccgga gaacaggcac ccgcccagga acgtgccctc cagtgcacgg tatccgtgca
      361 ctccgccccc gccgaatccg ctcgcctcgc cggcggcgaa cagtccgggg atcggcgcgc
      421 cgtcggcagc cagcgcgcgt ccgtccagat cggtctggat gccgccgagt gatttgcggg
      481 tcagcacgtg cagcttgacc gcgaccatgg gaccagccgc cggatcctgc agccgatgcg
      541 ggggagcggt gcggatcagc ctgtcgccgc ggtacgcccg catcgatcgc agcatggcga
      601 tctggccgtc cttcgtgaag tcgttgccga tctcgcggtc gcgcgccagg acctcctggc
      661 gagcccgatc cacatccagg gactctcctc ccggcatccg cttcatctgc gtcaggagcg
      721 actccagatc gttctcgacg acgaagtcgg cgccctcgtc caggaacgac tgcaccggac
      781 cggtcggacc cttcgcgagc cgcgacttca ccagcaggcc gacgtccttg ccggtgaggt
      841 cggggttctg ctcactgccg gagagcgcga actccttctc cacgatcttg agcgaggtga
      901 tgaaccagga gtggtcgtgc ccggtggtgc gcaggtgtgc gagggtgccg agcgtgtcga
      961 acccggggta cagcggcacc ggcaggcgcg tgccggtcgc gtccagccac agcgacgagg
     1021 ggccgggcag gatgcggatg ccatgactcg gccacaccgg atcccagttc tcgatgccct
     1081 ccacgtaatg ccacatccgg tcgccgttga tcaggtgcgc ccctgcctcc tcggagacgg
     1141 cgtgcatgga gccgtcgacg tacgcgggga cgcctgtgag catgtgctcc gggggcgtgc
     1201 ccagccggtc cggccaggct gcacgcacga gatcgtggtt gccgccgatg cctccggatg
     1261 ccacgatcac ggcatccgcg cggatctcga actccccgga gcgctcccgc gatgagggga
     1321 cgccgcgcgc ggtcagctcg tcggcgagga cgtcgccgct cgcacctgcc accgtgccgt
     1381 cctcgacgat cagcctcgtc accctgtggc gtgggaggat cgtcgcccga ccctccgctt
     1441 cggcgcgctc catggccgca gcgaagggtg cgacgatgcc ggggccggtg ccccaggtga
     1501 tgtgaaagcg cggcacggag ttgccagggc ctatcgcgcc gccgcctccc cgctcggccc
     1561 agcccacgac ggggaagaag ccgacaccgc gttcgcgcag ccagctgcgc ttctcgccgg
     1621 cggcgaactc caggtaggcg gacgcccagc ggcgcggcca ctcgtcctcc ggcctgtcga
     1681 acccggcgtt gccgaaccag tcctgggtgg cgagttcgag ggagtcgcgg atacccattc
     1741 gccgctgctc gggagagtcg acgaggaaca gcccgccgaa ggaccaccag gcctgtccgc
     1801 ccaggttcga gcgcggctcc tggtcgatga cggtcacgcg cctgccggcc tccagcgcct
     1861 ctgcagcggc gaccaggcct gacaggcccc agccgatgac gagcacgtcc gtgaggtgtg
     1921 tgcgggatgc catggcgact ccttcgtcgt cggtgagggg aggttacgag gtcagtccga
     1981 gatcgggacg gcgctcctgc gtgggcccga tcgtcgtcgg aggaggctca ggggagtcct
     2041 cgaacgtgtt cacgagcgag tgcgcggcgc gctgcagata gtcccagaac gtcgcatcgt
     2101 gcagggggga gagctgcgcc tcgtcgaggg cggtgcgcat gtgcgcgagc cagcggtcac
     2161 gggcatccgg gttgacgtgg aacggcacgt gccgcatgcg cagtcgcggg tgtccgcggc
     2221 gttcgctgta cgtgccgggt ccgccccagt actgctccag gaacatcgtc agccgctcgg
     2281 tggcggggcc gaggtcgtcc tccggataca tcggacgcat cacgggatcc actgccacct
     2341 cgcggtagaa cacctcgacg atgcgctgga aggtggcgtg accgccgatc tcgtcgtaga
     2401 acgtcacttg gcgtcgcctt ccttgtcgcc ggtcttcttg cgacgccaga ctccgcgctc
     2461 ggcgaccggg ggcacgcccg tgatcgcgtt cgggcgggtc ttgggcggat tggcgccgcg
     2521 cacacggcgt gcaccctcca gcccggagag ggtgacggat gccaggctgg gcacgagtac
     2581 gtccttctcc gtcagcgcct ggcgcagacg cgccctcagc tcctgcgcga cgtcgtccat
     2641 ggcgttggca cgggtcttca tcaccacgcg cacgaccagc gcgtcgccgg tgacggactc
     2701 caggccccag acctccgggc gctcgatgat gcgggtgcgc cacttcgggt ccttggcgag
     2761 tccctgcgcg gtctccagca tcgtggactc gacgagctcg agatccgcgt ccggctgcac
     2821 gccgagatcg atgatcgcgc gggcccagcc ctgcgacatg ttgccgatgc gcgtcacctc
     2881 gccgttgcgc acgtaccaga gcgtgccgtt cacgtcgcgc acctgggtga tgcgcacgct
     2941 gacgtactcg accacaccgg atgccaggcc cagatccacg acgtcgccga tgccgatctg
     3001 gtcctcggcg acgaggaaga ggccgttcag cacgtccttg acgatgttct gcgcgccgaa
     3061 accgaggccc gcacccaccg ctgcggtgag gagcgtcagc gagccgagca gggaaggact
     3121 cagctggttg accgtcagca cgatggccac gaccaccagg atcacgttga cgatgttctg
     3181 cagaatggtg ccgagcgtgc gcgtgcgctg cacgagccgc atgtccgcga ggggggagcg
     3241 ctccagtgcc tgggtgtcgt cgaccctggc cttgctcttg gcgctgtcca cgatgcgttt
     3301 gacgatgcgc cggatgacga ggcgcagcac gaacgccgcg accacgcagg acgcgatgat
     3361 gaccgcgacc cagatggcgt tcatgccgac ctcgccgagc acgccgagca ccttgtccca
     3421 ggtcgtgggc ttcgtcacat cttcgggggg tggggttgct gcgaggtgga gattcatcac
     3481 ctcgatgtta ccgagaaggc ctcggcgcac actgggtgcg ccgaggcctt ctcgcggagt
     3541 gggctgtgcg gatcagtcct cggcgtcccg ggactgcgcg gccagcgccc gctcgacgtc
     3601 ggcgaggttc tcgtgcacca gacggcgcag cgcgggcgac gcgtcctggt gcgacgacag
     3661 ccacgcgcgg gtcgcgtcgc gcagctcgac gctggccagc gaccgcgggt acaggcccac
     3721 gaccaggtag tcggcgatct ggtaggtgcg tccctcccag atgggcacga gcatgtcgaa
     3781 gtacttcggg atgaagtcgc cgagcacccc gacgcccgag gggtgcacga aaccgagtgc
     3841 cgccgagcgg acgatcgtgt tcggaaggtc ggcgttgtcg atcagcgatg accaggccgc
     3901 ctgcttcgcc tcagcggtcg gcagcgcggc gcgcgcctgc gcggcgaact ccgcgccctt
     3961 ggacgtgttg tctgcggcca gcgcggcatc gatcgctgcg gcatccgtcg ccccgatcgc
     4021 agccagaccg ttcagcagct gccatgacag gtccgcgtcg atgtccaggc ccggcagcgt
     4081 ctcctcgccg gagcgcaggc gaccgacgat gccggcgtgc tcgggggtga ccaggacgtt
     4141 cgcgaacgcc gtcaccagct ggagctggct gtcgctgccc ggctcggcct gctgcgcgag
     4201 cgcccacagg ccgtcggcca ccttctcccg cgccgcggtg cggttctcgg gagtgacgta
     4261 gctggaggcg gccagcagca gctgcgagag cgtggtgcgc accgtggtcg actcggtctc
     4321 gcgcccgatg ttgccgagca cgaggtcgat gtaggcggat gccgggctct cggcgtcacg
     4381 cgtctgatcc cacgcggcgc cccagacgag cgagcgcgcc agcgggtcgc tgatgtcgga
     4441 gaggtgctcg accgcggtgg ccagcgagcg gtcgtcgagc cggatcttcg cgtaagcgag
     4501 gtccttgtcg ttcaggagca ccatgtcggg gcgctgcaaa ctctgcagct ccgggatctc
     4561 cgtgcggtcg ccgtcgatgt cgacctcgac gtagtgcgtg cgcacgagcg cctcgccgtc
     4621 gaggttgtag aacccgatgc ccaggcggtg cgggcggatg gtcgggtagt cggcgggagc
     4681 ggtctgcgtg acggcgaagc gggtgatgac cccggctgcg tcctcggcga tgaccggcgc
     4741 gagcgtgttg acgcccgcgg tctccagcca cttcttcgcc caggtgctca gctcacggcc
     4801 gctggtggcc tcgagctcgg tgagcaggtc gctgagctcg gtgttgcccc aggcgtgctt
     4861 ctggaagtac tgcgagacac cggcgaagaa cgcctcgatg cccacccagg cggcgagctg
     4921 cttgagcacc gagccgccct tggcgtaggt gatgccgtcg aagttgacct gcacgtcctc
     4981 gaggtcgttg atctcggcga cgatcggatg cgtggagggg agctggtcct ggcggtatgc
     5041 ccaggtcttc tccatggcgg cgaaggtcgt ccaggcctcg gtccactcgg tggcctcggc
     5101 ggtggcgatc gtggacgccc actcggcgaa cgactcgttc agccacaggt cgttccacca
     5161 cttcatggtg accaggtcgc cgaaccacat gtgcgcgagc tcgtgcagga tcgtgacgac
     5221 gcggcgctcc ttgacggcat ccgtcacctt gctgcggaac acgtaggtct cggtgaacgt
     5281 gaccgcgccc gcgttctcca tggcgccggc gttgaactcc ggcacgaaga gctggtcgta
     5341 cttggcgaac gggtagggca cgccgaactt cgactcgaag tacgcgaagc cctcgcgcgt
     5401 cttgtcgaag atgtaatcgg cgtccaggtg cgcccacagg ctcttgcggc cgtagacgcc
     5461 gagcgggatg acccggcccg atgcactggt gagctcggag aaggtgacct cgtacggacc
     5521 cgcgatcagg gccgtgatgt acgaggagat ccgcggggtg ggctcgaagc cccaggtcgc
     5581 ggtctgctgg gtgccggagt ccgtcgacgc atcgtgcacg atcggctcgg gggtgggcga
     5641 gttcgacacg accttccacg cggcgggcgc ggtgaccgtg aactggaagg tggccttcag
     5701 atcgggctgc tcgaacacgg cgaacatgcg ccgcgagtcg ggcacctcga actgcgagta
     5761 gaggtagacc tcgccgtcga ccgggtcgac gaagcggtgc aggccctcgc cggagttcgt
     5821 gtacaggcag tcggcgtcga cgatgagcac gttctcggcc tgcagcccgc tcagcgcgat
     5881 gcgcgagtcg gcgaaggcct cgctcgggtc gatctgctca ccgttgagcg agatctcccg
     5941 tacctccttc gcgatcagat cgatgaaggt cgcgccgccg ggggtggcgg tgaagcgcac
     6001 gacgctgcgc gatccgaaca cctccgcgcc cttggtcagg tcgagcgaga cctggtagga
     6061 ctgcgtgtcg atgacggcgc ggcgctcctg cgcttcggta cgggtgaggt tctctccagg
     6121 cacagctgtt tctcccagtg gtgagggggt gtcgtccctc gtcggcactg ctggcgccga
     6181 cggcaaccct gacagcctac gccaggcgac gtctcgggca agcgactccg cgctggctca
     6241 tagccggcag tggaaggatg gagggcgtga tctctgaagc aacggatgtc gttctgcacg
     6301 cctcttccgc agcatccggg ggtgaaccgt tcatcgagca gccggtcgcg tacgacgcga
     6361 tcctcctggc cggattcggc ggacccgagg ggcaggacga ggtcatcccg ttcctgcgca
     6421 acgtgacccg tggacggggc atccccgacg agcgtctcga ggaggtcgcg caccactacc
     6481 gtcatttcgg tggggtcagc ccgatcaacg gacagaaccg tgcgctgaag gccgcactcg
     6541 aggccgaact ggaagcgcgc tcgatcgacc tgcccgtgta ctggggcaat cgcaactggg
     6601 cgccgtatct ggaggacgct gtggcggagg ccgccgggca cggcgacacc acgctcctgg
     6661 cgttcgcgac cagcgcgtac agctccttct ccagctgccg gcagtaccgc gaggacttcg
     6721 cgcgcgtact ggacggcacg gagtacgacg gggtcgtcac catcgacaag atccggccgt
     6781 tctacgacca cccgggcttc gtgcaggcgt tcgtcgaggg cgtgcacgac gccgtcgccg
     6841 cgttcctcgc cgagggcatc cccgcggacg ccgtgcaggt gctgttctcc acgcacagca
     6901 tcccgacggc ggatgccgag cgctccgggc cccgggacat cgacttcggc ccggggggcg
     6961 cctacgccgc gcagcacgag tccgtcgccg cctgggtgat ggatcgtgtc gcgcagctgg
     7021 agccggccgc ggcggacgtg ccatgggagc tcgtctacca gtcccgctcc ggccccgcct
     7081 cgcagccctg gctcgagccc gacgtctgcg acgtgatcgg cgagcttgcg gaacgcggca
     7141 ggcaggccgt ggcggtcgtg ccggtcggat tcatgagcga ccacatggag gtgctctggg
     7201 acctggacac cgaggcgaag gaggccgccg agcaggccgg cctgcgattc acgcggacgc
     7261 ccaccccggg cgtgtcgccg gcgttcgtgg ccgggatcgt cgacctcatc caggagcgcc
     7321 tggagggacg cccgaacgcg gaccgcgcgc gggtggcctc ggtgggcggt tcgttcgacg
     7381 tctgccgccc cggatgctgc gagaacgtgc gggccggatt caagccggcc gccgccggca
     7441 tcctgccctg agcggagcgg accggaaagt aggatgggcg ccatgcgcat ccacatcgcc
     7501 accgatcacg ccggtctcga cttctccacc cgcctgcagg agcatctgcg ttccgcaggg
     7561 cacgacgtga ccgatcacgg accggtggag tacgacgcgc tcgacgacta cccggcgttc
     7621 tgcatccgtg ccgcgcaggc cgtgatcgcc gaccagaccg ccggcgtcga ggcgctcggc
     7681 gtcgtgttcg ggggctccgg caacggcgag cagatcgctg cgaacaaggt gcagggcatc
     7741 cgcgcggcgc tggtgtggaa cacctccacg gcggagctcg cccgcgagca caacgacgcc
     7801 aacgtcatct cgatcggtgc acgccagcac accttcgacg aggtcacctc gttcatcgac
     7861 accttcatcg gcacgccgtt ctcaggcgag gagcgccacg tgcggcggat cgcccagatc
     7921 gccgacttcg agatctccgg ctcccttctg cccgatccgc gcgcctgaca tgcccgaggg
     7981 tcactccgtc caccgcatcg cgcgccagtt cgcgcgcaac ttcgtcgggc tcccggtgca
     8041 cgcgtccagc ccgcagggtc gcttcgccga gggcgcggcg gtgctcgagg gtcgcgagat
     8101 gctgcaggcg atggcggtgg gcaagcagat gttcctcgag ttcgagggcg acacctggct
     8161 gcgcgtccat ctcgggctgt acggcgcgtg ggacttcgcc ggggagatcc tcgtcgaccc
     8221 caccatcgca tccgccaacg ggcggatggg gcagaccaac cagcgcggca ccgatctgga
     8281 cggcgtgatc ctcgacgacg cgggggagaa ctcgctcacc tcgatcggcg ctccgcggaa
     8341 gacccgcgtg cacgtgcgca tgtcggagca gaccaagggg ctcgccgacg aggatgccga
     8401 gtggccgccg ccggtggtcg gccaggtgcg gctgcgcctg ctgaccgatg ccacctgcgc
     8461 cgacctgcgg ggtcccaccg cgtgcgtgct gcagacgccg gacgagatgc tggcaacgat
     8521 cgcgaagctg ggcccggacc cgctcgtcgg agaccccgcc gagggcgagg agcggttcgt
     8581 ccgcatcgtg cggcgcaagc ccaccgcgat cgcactgctg ctgatggatc aggcggtggt
     8641 cagcgggatc ggcaacgtgt accgcgccga gatgctgttc cgcgctcgcc tcaacccgca
     8701 cactccggga cgtgacgtcc cggaggaggt cgtgcggcag ctgtggcgcg actgggtccg
     8761 gctgctggcg atcggcgtgg agaccggcca gatgatgacc atggacgacc tctcgcccga
     8821 gcagtaccgg gccgccatgg ccagccgtga cgaccggcat tgggtgtacc gccgtgccgg
     8881 gctgccgtgc cgggtctgcg ggaccgagat cgctctggag gagctcggcg cccgaaagct
     8941 gtactggtgc ccgttctgcc agcgatgatg tgctgacacg ccgggatatt ggaacctgag
     9001 tgcgggctgt gaataggatg gccgaatccc ttcgcaacga cgcacgacag gagaagtcat
     9061 ggacctatcc ggcctgcagg ccgttctgga gaacgtcagc tcatggatct gggggccgtg
     9121 ggtcctcatc ccgctgctgc tcggaaccgg gctctacctc acgatccgcc tcggcggcat
     9181 ccagttcctc aagctcggcg cggccctgcg actgggcctg ttcacccgca aggaccccga
     9241 ctccgacggt gacatctcgc agttccaggc actgacgacg gcgctggcgg cgacggtggg
     9301 caccggaaac atcgtgggcg tcgccaccgc catcggcatc ggcggacccg gcgcactgtt
     9361 ctggatgtgg gtcaccggcc tgctcggtat ggcgtcgaag tactccgagg cgttcctggg
     9421 cgtgcgcttc cgcaccacgg atgccgcagg cgagaagtcc ggcgggccgc agtactacct
     9481 cgagcgcggc atcaagaacg ggttcggcaa gttcctcgcg atcttcttcg cgatctccgc
     9541 ggtgatcgcc tgcttcggca tcggcaacat gacccagggc aactcgatct ccgcgaacct
     9601 ggagaacagc ttcaacgtgc ccacctgggt caccggcctc gtgctcaccg tcttcgcact
     9661 gctcgtgctg gtcggtggca tcaagtccat cggtcgcgtc accgccggcc tcgtgcccgt
     9721 catgatcatc ttctacgtgc tcggcgcgat ctacatcctg atcgccaaca tcgccggtgt
     9781 tccggcggcc ttcgcgcaga tcttcagcga ggccttcacc ggcaccagtg ccgtgggcgg
     9841 cttcgcgggc tcgctcatca tcgtcgccgt gcagatgggc gtcgcccgcg gcatcttctc
     9901 caacgagtcc ggcatgggct cggcggcgat cgcggcggct gcggccaaga ccagccaccc
     9961 ggtccgtcag gggctcgtgt cgatgacgca gaccttcatc gacacgatca tcgtcgtcac
    10021 ctgcacgggt cttgtgatca tcaccaccgg cacctggaac gcagtggacg atgagggcaa
    10081 gcagatcagt cccgcgctca tgaccggcga ggccttctcg cacggacttc cgggtgaatg
    10141 gggccactac atcgtgacca tcggcctggt gctgttcgcc ggctccacga tcctcggctg
    10201 gtcgtactac ggagagcgca gcctggagcg gctggtcggg gtgaaggcga tcatgcccta
    10261 ccggatcatc ttctcgctgg tggtgttcgt cggctgcacg accgagctcg gcgtggtgtg
    10321 ggcgttctcc gatgtgatga acggcctgat ggcgctgccg aacctgatcg gcctgctggt
    10381 gctctcgggc ctggtcgccc gcgagaccaa gaagtatctg accaacgatc cgaagctcac
    10441 cgcgacgacc gacgaggtca acgccttcat ggcgggcgat gcgggtttcg aggactggaa
    10501 gacccaggcg gttccggtcg tcaaggcgag gtagcggcgg tggcgggcgg ggcacgacga
    10561 ccctgcccgc cccatcggtc ctaatctgga accatgcgcc agaacccgag cttcgccatg
    10621 acggacatcg ccgagctgcg gagggtcatc tcgcagagcc cctgggcgac gctggtcgcg
    10681 accggtgacg acgggctggt cgcatcgcac tatcccgtgc tgcttgaccc cgagcgcgac
    10741 gacctcacgg tggtcggtca cgtcggcaag cccgacgacc aggtgctgca cctgggggag
    10801 cgggagctgc tgatgatctt ccagggtccc cacgggtaca tctcgccgag ctggtatggc
    10861 gacgttcggg cggtgccgac ctggaactac acggcggtgc acctgaccgg catcccggag
    10921 gtcgtcgaca cgcaggcgaa cctgaggatc ctcgacgatc tcgtcacgca tttcgagcaa
    10981 aggctgccgc agccgcgcat gatgtggcag gcgccgaaca ccgaggagtt cgtgaacgcc
    11041 ctggagatgg ggacggtcgg attccggatg acccccacgc gggtctcggc gaagcgcaag
    11101 ctgagtcaga acaagccgga cagcatcgtg gagaacgtga tcgcagagct cgacggcagt
    11161 ggtgagtacg cgcatccggc cctggcggcg gagatgcgcc gcgcgcacga ggcacgtctg
    11221 gcgcaccgcg gatgagcgcc gatctgctga tccggtccgt gcgggtcgcc ggtgccggcc
    11281 gcgagctgct gccgttcgcc tcgccggacg gagacgaacc cgtcgacatc ctggtctccg
    11341 gtggcgtgat cgccgacatc gccccggccg gcaatctccg agccacggcg gatgcgctcg
    11401 acggcgacgg cacctgggtg gtgccgggac tgtgggacca ccacgtgcac accgtgcagt
    11461 gggcgctcga cgcacagcgt gtgccgctcg gtggcgcgac gagcgcggcc gag
//
LOCUS       DATMSX010000311         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_118625,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000311 DATMSX010000000
VERSION     DATMSX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118625"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN041_09470"
     CDS             complement(<1..524)
                     /locus_tag="VN041_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29303.1"
                     /translation="MSAAGTLSPRTPDQPMAFTRDEFVMGAVASWLFFLGLLTVTEVA
                     IAVVSDVMASVRGGSSGFSSLGLLVPVLLISLLIAAPVSGIATLLAAPAIRRVAWRLR
                     ARRSPWLHAAVHAAFGAAVGALTIVVLQACFLLWTGAAAPLPQFGGYCLIAAVLTSAS
                     TVLGWAVTARNARR"
     gene            complement(521..1189)
                     /locus_tag="VN041_09475"
     CDS             complement(521..1189)
                     /locus_tag="VN041_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29304.1"
                     /translation="MFSSELTPKAEQTRARIRVAAIESFIERGYPDTTIRLIAREAGV
                     SVGNAYYYFPSKTHLVQELYEQVQQDHARVAVPLLEGVTGLVDRLRIVFETGLATLEP
                     YQRVAPGFLSAMIAPDSPINPLARESSGAREMTIALFRRAVDGAEHRLPEDVAKRLPE
                     ALFVAFLGLIVRWTYDEDDHTRTRRLLDTALKLLAVALPFVRVPGVRGVARDLLDQIA
                     EVRA"
     gene            complement(1235..>2035)
                     /locus_tag="VN041_09480"
     CDS             complement(1235..>2035)
                     /locus_tag="VN041_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU29305.1"
                     /translation="QDVLTGERLTIRARSVINATGVWAGSLDDAITVRPSRGTHIVLD
                     GADLGHPRAALTIPHVGSVSRYVFALPQPGGRVYVGLTDEDAPGDVPRVAQPTEPEIA
                     FLLESLNRVLQTPVHPGQVLGAFAGLRPLVDSGGGSTADVSRRHLVAASDDGFVSVLG
                     GKLTTYRRMAEDAVDLAVRARGLTAPPSRTASLRLASFCRVDPAPPLAEGLTLTAADV
                     TDAVRTQGAMTADDVLDRRTRVGLVPDERARLIPSVADLVARTLDELG"
BASE COUNT          336 a          703 c          740 g          256 t
ORIGIN      
        1 caccggcgcg cgttgcgcgc ggtgacggcc cagccgagca ccgtggacgc ggaggtgagc
       61 accgcggcga tcaggcagta gccgccgaac tgcggcaatg gtgccgcggc acccgtccac
      121 agcaggaagc acgcctgcag gacgacgatg gtcagtgctc cgaccgccgc gccgaaggcg
      181 gcgtggacgg cggcatgcag ccaaggggag cgccgcgcac gcagccgcca cgcgactctg
      241 cggatcgccg gcgccgcgag cagcgtcgcg attccggaga ccggcgccgc gatcagcagc
      301 gagatgagca gcacggggac cagaagcccc agcgaggaga agccggagga gccaccgcgc
      361 accgaggcca tgacatcgga gacgacggcg atggcgacct ccgtgaccgt gagcagtccg
      421 aggaagaaga gccaggacgc caccgccccc atgacgaact cgtcccgcgt gaacgccatc
      481 ggctgatcag gggtgcgcgg cgacagtgtt cccgccgcgc tcatgcccgc acctccgcga
      541 tctgatcgag caggtcgcgt gcgactccgc gtacgccggg gacgcgtacg aacggcaggg
      601 ccaccgcgag cagcttcagg gcggtgtcca gcaggcggcg agtgcgggtg tggtcgtcct
      661 cgtcataggt ccagcgcacg atgagtccga ggaaggcgac gaagagggct tccgggagcc
      721 gcttcgcgac gtcctccggc agccggtgct cggctccgtc caccgcgcgc cggaagagcg
      781 cgatcgtcat ctcccgcgca ccggatgact ctcgcgccag cgggttgatg ggggagtccg
      841 gggcgatcat ggcgctgagg aagcccggcg ccacgcgctg atagggctcc agcgtggcga
      901 ggccggtctc gaagacgatc cgcaggcggt cgaccaggcc cgtgaccccc tccagcagcg
      961 ggacggcgac gcgagcgtga tcctgttgca cctgctcgta cagctcctgc accaggtggg
     1021 tcttcgaggg gaagtagtag tacgcgttcc cgactgacac ccccgcttct ctggcgatca
     1081 gccggatcgt cgtgtcgggg tagccgcgct cgatgaagga ctcgatcgcc gccacccgga
     1141 tgcgggctcg cgtctgctcg gccttcgggg tgagctcaga actgaacatg ttcaaataat
     1201 agcgccaacc aagggtcgaa ggcgaggcgc gcagtcagcc gagttcgtcg agggtccgcg
     1261 cgacgaggtc ggcgaccgac gggatcagtc gtgcccgctc gtccggaacc aggcccacgc
     1321 gggtcctccg gtcgagaacg tcatccgccg tcatggcgcc ctgagtgcgc acagcatccg
     1381 tgacgtcggc cgcagtcagg gtcaggccct ccgccagcgg aggcgcggga tcgacgcggc
     1441 agaaggatgc cagtcgcaga gaggccgtgc ggctgggcgg ggccgtcagg ccgcgcgccc
     1501 gcacggccag atccacggca tcctccgcca tccggcggta tgtggtgagc ttgccgccca
     1561 gcaccgagac gaagccgtcg tcggacgccg cgacgagatg cctgcgggac acgtcggccg
     1621 tgctcccgcc gccgctgtcc accaggggac ggagtccggc gaacgcaccg agcacctgcc
     1681 ctgggtggac cggcgtctgc agcacccgat tcaggctctc gagcaggaac gcgatctccg
     1741 gctccgtggg ctgcgcgacg cgcggcacgt cgcctggcgc gtcctcgtcg gtgagcccga
     1801 cgtagacccg cccgcccggc tgcggaagag cgaacacgta ccggctgacg gagccgacgt
     1861 gcgggatggt cagggcggcg cgcggatgcc cgagatcggc gccgtcgagc acgatgtgcg
     1921 tcccgcggct gggacgcacc gtgatcgcat cgtcgagcga gccggcccac actcccgtgg
     1981 cgttgatcac gctgcgcgcg cggatcgtca gccgctcgcc ggtgagcaca tcctg
//
LOCUS       DATMSX010000312         7067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_23579,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000312 DATMSX010000000
VERSION     DATMSX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23579"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VN041_09485"
     CDS             complement(<1..657)
                     /locus_tag="VN041_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU29306.1"
                     /translation="MSVQLRVVLDQASQVVDADQARAALSLTAGLVATAPRGCVVSAL
                     VPRGAEVPVHGVQDVRGLPLGRQELAATWQLGIVPGVGGGLIHAPSLMAPLVRHDRAN
                     DNDQVTVTLWDLDAWEAPERLSKAQLVWQKAMLKRAAKHADAVVVPSHSMAERLGELG
                     RFGDRVRVIAGAAPEGFAVPADAPRRREDLQIPGRYIALVGDADSLLQGFRAAAQAGA
                     D"
     gene            complement(715..2169)
                     /locus_tag="VN041_09490"
     CDS             complement(715..2169)
                     /locus_tag="VN041_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWU29307.1"
                     /translation="MTLAAPRAPEAGARPLIESRPIRNPDQQDSHSMGHRAWWLVVLN
                     VLIPGSAQVLAGNRRLGRFGLGATLLGWFLVIVAVGFALFARQTLVWLATGPMSWFVL
                     TLAQVLCLAYALLWVVLTVDTLRLLRLVKLPTITRWAAPIASLVVLALVGSGAVYASN
                     IAAGSRGALNALFGNSGPSLPPSEGYYNVLLLGADSGDGRDSMRFDSISVVSVNATTG
                     ATTITGIPRELPDPPFSADSPMHKLYPDGFRSHGSSTCGWNAWMNQIRNAAEVCRKDR
                     GKALYPDAAAHGSAPGIEATKDAAEAVLGIKIPYYVFVDMNGFADLVDALGGVDINVT
                     ERLPKGGPPPGSGAHDVDKWAIGWIEPGQQHMNGDTAQWYARSRYTTSDYDRMKRQRQ
                     LQEAILAQFTPENVLTHFSEVMKAGTALASTDLPQDKLPEFFDLMMKAKKQPVTQIEL
                     TPENGIEEKTPDYDLIREKVKDALHPPTKAPSEG"
     gene            complement(2166..2633)
                     /gene="purE"
                     /locus_tag="VN041_09495"
     CDS             complement(2166..2633)
                     /gene="purE"
                     /locus_tag="VN041_09495"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWU29308.1"
                     /translation="MGSDSDWRVMSDASQALTDFGIPHEVEVVSAHRTPDKLMSYARE
                     ARGRGIRVIIAGAGGAAHLPGMLASMTALPVVGVPVPLAYLDGMDSLLSIVQMPAGIP
                     VATVSIGGARNAGILAARILGTSDAELADRVEAYARDLEGQVEQKNRRLKDSL"
     gene            complement(2751..3875)
                     /locus_tag="VN041_09500"
     CDS             complement(2751..3875)
                     /locus_tag="VN041_09500"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWU29309.1"
                     /translation="MTVRVGVIGGGQLARMMIAPAVELGIDLRVLAEDHGMSAQLAAT
                     AVGDYRDLDTVRAFVRDVDVVTFDHEHVPQDVLRALVADGVQVHPGPDALQFAQDKLV
                     MRARLAELGVPQPDWAAVSDAEQLQRFIDEHGGGAVVKTPRGGYDGKGVRVVRAGDEA
                     ADWFAGLAEGESLLAEELVSFTRELAQQIARRPGGEMVAYPVVETVQRDGVCAEVIAP
                     APGTSARLVQVAEQIGRTIAEGVGVTGMLAVELFETDDERILVNELAMRPHNSGHWSQ
                     DGAITGQFEQHLRAVADLPLGRTEPRAAWTVMVNILGGPAEGALEDRFTRAMTEHPEA
                     KIHTYGKAPRPGRKVGHVNVAGSDLDDVVYVARAAAAQFL"
     gene            complement(3952..4461)
                     /locus_tag="VN041_09505"
     CDS             complement(3952..4461)
                     /locus_tag="VN041_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWU29310.1"
                     /translation="MPPPGAPVEELLVARFRSHARRLFWSALVLIGVCGATAFLYGNL
                     EPPFEDWMLLAAAAAAVVLLVIVPFLVWLSRTYTITTRRVIVREGMGSRGRRELSHTR
                     GYSIAVRRGILQRMWGAGTITLSNGVDAPLRLVNVPSVNLVHEALADQIEVNQILAHR
                     DAQSGTAGD"
     gene            complement(4555..5319)
                     /locus_tag="VN041_09510"
     CDS             complement(4555..5319)
                     /locus_tag="VN041_09510"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWU29311.1"
                     /translation="MDLTRTSAAASRLLVLPECGSTNAELRGRAADVVAWPHLSALLT
                     DTQTSGRGRLDRTWTTPAGAALAVSVLLRDLPADVELWGWIPLAAGVGMTDAVSAQLP
                     GHEVGLKWPNDVLVDGRKICGILAEVTGGAIVLGTGINTTMTAEQLPVPTATSFAALG
                     VEVDIDRLLSDYLTGLAAVVDALAQQADAAASGLHDAATSRCLTLGQDVDVSLPDGSV
                     LTGRAVRLASDGRLVVDDGRTEHEVASGDVVHVRPA"
     gene            complement(5320..6603)
                     /locus_tag="VN041_09515"
     CDS             complement(5320..6603)
                     /locus_tag="VN041_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWU29312.1"
                     /translation="MGVVSDGKKAYRLLRKALTSRSAVQRVRRTLAAQEPHEPGRYRV
                     AVYFADGAVNIYQMRQWYRPLQGLAERWPVVVLSRSATGAEKLIEESGLPVAFVPTVR
                     NLEKFLAKQDIRIVLYVNQNTRNFQMFRYGRRWHVFINHGESDKMYMTTNQYKAYDYA
                     LIAGQAARDRLSRTLWDFDLDRRTIEIGRPQADHYSGTLPYTPDERTVVLYAPTWEGD
                     RPSAHYGSIATHGEALMRGLLASPRHRVIYRPHPRSGVVNEEYGAAHQRILDAIAAAN
                     AADPSAHHVYDDGPDLGWQLAAADVAVVDISAMVYDRLAAGKPLMITRPADERASIDT
                     TGYLSACEWLTVSGAENILAEVERVSADAEAVARLRTWVQHYFGDTTPGAATAKFHGA
                     IEQLMQKWEEWHARDAVESGPDDDDVSDDDEDDED"
     gene            6751..>7067
                     /locus_tag="VN041_09520"
     CDS             6751..>7067
                     /locus_tag="VN041_09520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU29313.1"
                     /translation="MPVLNEIDYLEHAVQSVLAQDVGAPTELVLALGPSSDGTTALAQ
                     RLADGDARIRLVQNPHAHIPVGLNAAIRASRYPTIIRVDAHSELSPGYARRALETLQR
                     TGAA"
BASE COUNT         1053 a         2552 c         2407 g         1055 t
ORIGIN      
        1 atccgcaccg gcctgagccg cggcgcggaa accctgcagc agggagtccg cgtcgcccac
       61 cagcgcgatg taacgcccgg ggatctgcag gtcctcgcga cgccgcggtg cgtccgcggg
      121 aaccgcgaac ccctccggag ccgcgcccgc gatcacccgg acccggtctc cgaagcgccc
      181 gagctcaccc aggcgctccg ccatcgaatg cgacggcacc acgaccgcat ccgcgtgctt
      241 ggccgcacgc ttgagcatcg ccttctgcca gaccagctgc gccttgctca gcctctcggg
      301 cgcctcccac gcatccaggt cccacagggt cacggtgacc tggtcgttgt cgttcgcgcg
      361 atcatggcgc accagcggcg ccatcaggct cggcgcgtgg atcagcccac ctccgacgcc
      421 cggcacgatg cccagctgcc acgtcgcggc gagctcctgc cgccccaggg gcagaccacg
      481 cacgtcctgc acgccgtgca cgggcacctc ggcacctctc ggcacgagcg ccgagacgac
      541 gcaccctcgc ggcgcggtgg cgaccagccc ggccgtcaag gagagcgccg cgcgcgcctg
      601 atccgcgtcc acgacctgcg acgcctgatc cagcaccacc cgcagctgca cactcataag
      661 gtcagcctaa gcgagccgcc ccgatatacc gggcgaccgg ccgcccttcc ccgatcagcc
      721 ctcgctcggg gccttcgtcg gcggatgcag cgcgtccttc accttctcgc ggatcaggtc
      781 gtagtcgggg gtcttctcct cgataccgtt ctcgggcgtc agctcgatct gcgtgacggg
      841 ctgcttcttg gccttcatca tcagatcgaa gaactccggc agcttgtcct gcggcaggtc
      901 cgtggacgcg agcgccgtac cggccttcat cacctcggag aagtgcgtga gcacgttctc
      961 cggtgtgaac tgcgcgagga tcgcctcctg cagctgccgc tgccgcttca tgcggtcgta
     1021 gtcgctcgtg gtgtaccgcg agcgggcgta ccactgcgcg gtatccccgt tcatgtgctg
     1081 ctggccgggc tcgatccagc cgatcgccca cttgtccacg tcgtgcgcgc cggatccggg
     1141 cggcgggccg cccttcggca gacgctcggt cacgttgatg tcgacgccgc ccagcgcgtc
     1201 cacgagatcg gcgaagccgt tcatgtcgac gaacacgtag tacgggatct tgatcccgag
     1261 cacggcctcg gcggcatctt tggtcgcctc gatgcccggc gcggagccgt gcgccgcggc
     1321 gtccgggtac agcgccttgc cgcggtcctt gcggcacacc tcggcggcgt tgcggatctg
     1381 gttcatccag gcgttccagc cgcacgtgct ggagccgtga gacctgaacc cgtccggata
     1441 cagtttgtgc atggggctgt cggccgagaa cggcggatcg ggcagctcac gcgggatgcc
     1501 cgtgatcgtc gtcgcgccgg tggtggcgtt gacggacacc accgagatgc tgtcgaagcg
     1561 catggagtcg cggccgtcac cgctgtcggc gccgagcagg agcacgttgt agtagccctc
     1621 gctgggcggc aggctgggtc cgctgttgcc gaacagcgcg ttcagcgcgc cccggctgcc
     1681 cgcggcgatg ttcgaggcgt agacggctcc ggagccgacc agcgcgagca cgaccaggga
     1741 tgcgatcggc gccgcccagc gggtgatcgt gggcagcttc acgagcctga gcagccgcag
     1801 cgtgtccacc gtgagcacga cccacagcaa tgcgtaggcc aggcagagca cctgtgccag
     1861 cgtgagcacg aaccacgaca tgggcccggt cgccagccac accagggtct gccgcgcgaa
     1921 cagggcgaac ccgaccgcga cgatcaccag gaaccagccc aggagcgtcg ctccgagccc
     1981 gaaccttccg agccgtcggt tgcccgcgag cacctgcgcg gaaccgggga tcagcacgtt
     2041 cagcaccacg agccaccagg cacggtgccc catgctgtgg gagtcctgct gatccgggtt
     2101 gcggatcgga cgcgactcga tgagcggccg tgcgcccgcc tcaggggcgc gcggagccgc
     2161 cagggtcaca gcgagtcctt cagccgccgg ttcttctgct ccacctggcc ctcgaggtcg
     2221 cgcgcgtacg cctcgacccg atccgccagc tcggcgtccg aggtgcccag gatgcgagcg
     2281 gcgaggatgc ccgcgttccg cgccccgccg atggacaccg tcgccaccgg gatgcccgcc
     2341 ggcatctgca cgatcgacag cagggagtcc atgccgtcga gataggcgag cggcaccggc
     2401 acgccgacca cgggcagtgc cgtcatggag gcgagcatgc ccggcaggtg ggctgcgccc
     2461 ccggcgccgg cgatgatcac gcggatgccg cggccgcgcg cctcgcgcgc atagctcatc
     2521 agcttgtccg gtgtgcggtg tgcggagacg acctcgacct cgtgcgggat gccgaagtcg
     2581 gtgagcgcct gcgatgcgtc gctcatcacg cgccaatcgg agtcggaacc catcacgacg
     2641 ccgaccaggg gcgaactgga ggaatgcagc ggctcagtca ccatgacagg ctacggggcg
     2701 cccctgtgag aacgtcgcaa cggcacgcag tcgcgctgtg agagccgggc tcagaggaac
     2761 tgcgcggcag cggcgcgcgc gacgtacacg acatcgtcaa ggtcggagcc cgccacgttg
     2821 acgtgaccga ccttgcgacc gggccgcgga gccttgccgt aggtgtggat cttcgcctcg
     2881 ggatgctcgg tcatcgccct cgtgaaccga tcctccaggg ccccttcggc gggccccccg
     2941 aggatgttca ccatcacggt ccacgcggcg cggggctcgg tgcgcccgag cggcagatcc
     3001 gcgacggcgc gcaggtgctg ctcgaactga ccggtgatcg caccgtcctg gctccagtgg
     3061 ccgctgttgt gcgggcgcat ggcgagctcg ttcacgagga tccgctcgtc gtccgtctcg
     3121 aacagctcga cggccagcat tccggtgacg cccacgccct cggcgatggt gcggccgatc
     3181 tgctcggcga cctgcaccag gcgggcgctg gtgcccggcg cgggtgcgat gacctctgcg
     3241 cacacgccgt cgcgctgcac ggtctccacg accggatagg cgaccatctc gccaccggga
     3301 cgacgagcga tctgctgcgc gagctcgcgc gtgaacgaga cgagttcctc ggcgagcagc
     3361 gactcgccct cggcgaggcc tgcgaaccag tcggctgcct cgtcgcctgc gcgcacgacg
     3421 cgcacgccct tgccgtcgta gccgcctcgg ggcgtcttca cgacggcacc tccgccgtgc
     3481 tcgtcgatga agcgctgcaa ctgctcggca tccgacaccg ccgcccagtc cggctgcggt
     3541 acgccgagct cggcgaggcg cgcgcgcatg accagcttgt cctgcgcgaa ctgcagagcg
     3601 tcggggccgg ggtgcacctg cacgccgtcg gcgacgaggg cgcgcagcac gtcctgaggg
     3661 acgtgctcgt ggtcgaaggt gacgacgtcg acgtcgcgca cgaacgcgcg cacggtgtcc
     3721 aggtcgcgat agtcgccgac cgcagtcgcg gccagctgcg ccgacatgcc gtggtcctcg
     3781 gccaggacgc gcagatcgat gccgagctcc accgccgggg cgatcatcat cctcgccagc
     3841 tggcctccgc cgatcacacc cacgcgcaca gtcatccgcc gtcctttcgt caacgaccat
     3901 tcttccgcat gcgggaagcg tgcggatgcc gttcgaaccg cgtccccggg ctcagtcgcc
     3961 cgcggtgccg gactgcgcgt cgcgatgcgc gaggatctgg ttgacctcga tctggtcggc
     4021 cagcgcctca tgcaccaggt tcacgctcgg cacgttgacc aggcgcagcg gggcgtccac
     4081 gccgttgctc agcgtgatgg tccccgctcc ccacatccgc tgcaggatgc cccgccgcac
     4141 ggcgatcgag tagccccgcg tgtgcgagag ctcccgcctg ccgcgtgacc ccatcccctc
     4201 gcgcacgatg acgcggcggg tggtgatcgt gtaggtgcgc gacagccaga cgaggaaggg
     4261 gacgatcacg agcagcacga ctgcggcagc cgccgcggcc agcagcatcc agtcctcgaa
     4321 cggcggctcg aggttcccgt agaggaaggc tgtcgcgccg catacgccga tcaggaccag
     4381 ggcggaccag aacagacgcc gcgcatggct gcggaaccgc gccaccagca gctcttccac
     4441 gggcgcgccc ggtggcggca tcatcggccg tccgccgagc gtcacgggct gggtcacccc
     4501 tccattgtgc ccgtacggct cgtcgtcgag ctcagggatc caggtgtgtc ctgatcaggc
     4561 cgggcgcacg tgcacgacgt cgcccgaggc cacctcgtgc tcggtccgtc cgtcgtcgac
     4621 gaccaggcgg ccgtcggatg cgagacgcac ggcccgtccc gtcagcacgc tgccgtccgg
     4681 cagggacacg tccacgtcct ggccgagcgt gaggcagcgc gaggtcgcgg cgtcgtgaag
     4741 tccggatgcc gcggcatccg cctgctgtgc gagcgcgtcg accacggccg ccaggccggt
     4801 caggtagtcg gagagaagcc ggtcgatgtc gacctcgacg ccgagcgccg cgaaggaggt
     4861 cgcggtgggg accggcagct gctcggcggt catggtggtg ttgatccccg tgccgaggac
     4921 gatcgcaccc ccggtgacct cggcgaggat cccgcagatc ttgcgtccgt cgacgagcac
     4981 gtcgttgggc cacttcagtc ccacctcgtg accgggcagc tgcgcggaga cggcatccgt
     5041 catccccacc ccggccgcca gcgggatcca gccccacagt tcgacatccg cgggcaggtc
     5101 gcgcagcagc acggagacgg cgagcgcggc tcccgccggt gtggtccagg tgcggtccag
     5161 gcgcccgcgc cccgaggtct gcgtgtcggt cagcagcgcc gagaggtgcg gccaggcgac
     5221 cacgtccgcg gcccggccac gcagttcggc gttggtcgag ccgcactcgg gcagcacgag
     5281 cagtcgcgag gcggcagcgg aggtcctggt caggtccatt cagtcctcgt cgtcctcgtc
     5341 gtcgtcgctc acgtcgtcgt catccggccc ggactccacg gcgtcacgcg cgtgccactc
     5401 ttcccacttc tgcatcagct gctcgatggc accgtggaac ttcgcagtgg cagcacccgg
     5461 cgtcgtgtcg ccgaagtagt gctgcaccca ggtgcgcagg cgggcgacgg cttcggcatc
     5521 ggcgctcacc cgctcgacct cggcgaggat gttctccgcg ccggagaccg tgagccattc
     5581 gcacgccgac aggtatccgg tcgtgtcgat cgaggcgcgc tcgtcggcgg gccgcgtgat
     5641 catgaggggc ttgcccgccg cgagccggtc gtagaccatg gccgagatgt cgaccaccgc
     5701 gacatcggcg gccgcgagct gccaaccgag gtccggaccg tcgtcgtaca cgtgatgcgc
     5761 ggacggatcg gcggcgttgg ccgcggcgat cgcgtcgagg atgcgctgat gcgcagcgcc
     5821 gtactcctcg ttcaccacgc cgctgcgcgg atgcggacgg tagatgacgc ggtgtcgggg
     5881 gctggcgagg aggccgcgca tgagcgcctc accgtgcgtg gcgatcgagc cgtaatgcgc
     5941 ggacgggcga tcgccctccc aggtgggcgc gtacagcacc acggtgcgct catccggggt
     6001 gtagggcagc gtccccgaat agtgatcggc ctgcggacgg ccgatctcga tcgtgcgacg
     6061 gtcgagatcg aagtcccaca gcgtgcggct gagccggtct cgggcggctt gacctgcgat
     6121 cagcgcgtag tcgtacgcct tgtactggtt cgtggtcatg tacatcttgt cggactcgcc
     6181 gtggttgatg aacacgtgcc agcggcgtcc gtaccggaac atctggaagt tgcgggtgtt
     6241 ctgattcacg tacagcacga tgcggatgtc ctgcttggcg aggaacttct cgaggttgcg
     6301 caccgtgggc acgaacgcca ccggaagccc gctctcctcg atcagcttct cggcgcccgt
     6361 cgcgctgcgg gagagcacga ccaccggcca ccgctctgcg agcccctgca gtgggcgata
     6421 ccactgccgc atctggtata tgttcacggc gccgtcggcg aagtacaccg caacccggta
     6481 gcggcccggc tcgtgcggct cctgagcggc gagcgtgcga cggacccgct gcacagctga
     6541 gcgcgaggtg agcgccttcc gcagcagccg gtacgccttc ttcccgtcag agaccacacc
     6601 catccgtcca gaatacgggg tgggatgcct tccctccgag ccggtgacag ggcaacctcc
     6661 gaatcctccg agtcgccgaa ccgcgccgca tgacatgatc gacgggtgca gcacaacgac
     6721 aggaccgact ccgacggcgt ctccttcgtc atgcccgtgc tgaacgagat cgactacctg
     6781 gagcacgcgg tgcagtccgt gctggcgcag gacgtgggcg cgccgaccga gctggtcctg
     6841 gcgctgggac cctccagcga tgggacgacc gccctcgctc agcgtctcgc cgacggcgac
     6901 gcccgcatcc gcctggtgca gaacccgcac gcgcacatcc cggtcggtct gaacgccgcg
     6961 atccgcgcga gccgataccc cacgatcatc cgcgtcgacg cgcactccga gctctccccc
     7021 ggctatgcgc ggcgcgcgct ggagacgctg cagcgcaccg gcgccgc
//
LOCUS       DATMSX010000313         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_23581,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000313 DATMSX010000000
VERSION     DATMSX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23581"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VN041_09525"
     CDS             complement(<1..154)
                     /locus_tag="VN041_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWU29314.1"
                     /translation="MTSLRVLGIDPGLTRCGVGIVDVDRTRRATLVHVGVVRTPLDAE
                     LPVRLAA"
     gene            complement(219..980)
                     /locus_tag="VN041_09530"
     CDS             complement(219..980)
                     /locus_tag="VN041_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29315.1"
                     /translation="MSGHSKWATTKHKKAVIDARRAKSWAKLIKNIEVAAKLGGADLD
                     GNPTLFDAVLKAKKTSVPKDNIDRAIKRGAGIGGEAVEYTSIMYEGYGQGGVALMIEC
                     LTDNKNRAAAEVRTALSRNGGTLADPGSVAYNFTRKGVIVVGSEGTTEDDVMMAALEA
                     GAEEIEEHAEGFQVITDASDLVPVRSALQEAGIDYESADVEFVPNLKVEIDADTARKI
                     FRLIDALEDSDDVQNVYANFDLTAEVQAELESDDE"
     gene            complement(1058..1933)
                     /locus_tag="VN041_09535"
     CDS             complement(1058..1933)
                     /locus_tag="VN041_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HWU29316.1"
                     /translation="MRLPLGSTDVSASGGTVTLVGRRGEAWEALCARSIPLDEAVELL
                     VDQHEGAHAGDEFADAVPADSEDAPRFTAGEPILWRYGRYIETARVIRDDARGLVVWI
                     PSGSARLESVPADGRATREVPLEERFSVPWEIRESTWRGPGVVRVAPSGRPWSVWFFR
                     GADGTPEGAYVNLELPHLRSATGAPGIFSRDLILDLWIEADHPGEEDVWLKDADELAA
                     AVAQGRFTAAQADAVRAIADHAAAEFLMPGGWPLDEGWAHWTPDAAMDEPIALPESAA
                     VAEARGRTGGTRLNG"
     gene            2029..2760
                     /locus_tag="VN041_09540"
     CDS             2029..2760
                     /locus_tag="VN041_09540"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWU29317.1"
                     /translation="MDMLKGEQIAAAALTDWRKLAQGLYARYLVRDYRSAARFVTAIA
                     EAGARIGHHPRTTIGEGHVDLRLVSVDAVYRDGEGVEHVVEWVTQNDVDLARAITEIA
                     AGQGIAPEPTAVSEIELGLDTPDSGMIAPVWAALLTGHAESRGRGTPSDEIRDATGRV
                     PHLWFGDADDASPQRFHVEVYVPPECVAERVAAVKDAGGVVIDDSDAPGLTVFADQDG
                     NRGVLCADTSAASRPAGDAGAVRTA"
     gene            complement(2767..3360)
                     /gene="pdxT"
                     /locus_tag="VN041_09545"
     CDS             complement(2767..3360)
                     /gene="pdxT"
                     /locus_tag="VN041_09545"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWU29318.1"
                     /translation="MTTARVGVLALQGDVREHVALLEHLGADVVRVRRPEEFAQVDGL
                     AIPGGESTVIDKLSRQFGLAEPIRAAIRDGMPMLGTCAGLIMLSDRLIDAIDGQQTFG
                     GMDVLTRRNAFGRQTESFEAELDIPALGTDPVHTTFIRGPVVEEVGADATTLAALEDG
                     RVVAVEQGNLLGISFHPELSGEPRFHQRFLDRVAARG"
     gene            complement(3357..4253)
                     /gene="pdxS"
                     /locus_tag="VN041_09550"
     CDS             complement(3357..4253)
                     /gene="pdxS"
                     /locus_tag="VN041_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWU29319.1"
                     /translation="MADQTPTTGSQRVKRGLAEMLKGGVIMDVVTADQAKIAEDAGAV
                     AVMALERVPADIRAQGGVSRMSDPDMIDSIIDAVSIPVMAKARIGHFVEAQVLQELGV
                     DYIDESEVLSPADYVNHIDKWGFTVPFVCGATNLGEALRRINEGAAMIRSKGEAGTGD
                     VSEAMKHIRKIRGEIAALGALSKDELYVAAKELQAPYELVSEIAETGKLPVVLFVAGG
                     VATPADAAMMMQLGADGVFVGSGIFKSGDPVARAKAIVKATTFYDDPKVIAEVSRGLG
                     EAMVGINVADLPAPHRLAERGW"
BASE COUNT          658 a         1569 c         1526 g          702 t
ORIGIN      
        1 ccgccgccag gcgcaccggg agctccgcgt ccagcggtgt ccgcaccacg cccacgtgca
       61 ccagtgttgc gcgccgcgtc cggtcgacgt cgacgatgcc gacgccgcac cgggtgaggc
      121 cgggatcgat gcccagcacg cgcagagagg tcactccccc aggctacggg cgcagaacgg
      181 ccccggacgc aggcgcgccc ggggccgttg cgaagctctt actcgtcgtc gctctcgagc
      241 tcggcctgca cctcggcggt caggtcgaag ttggcgtaga cgttctgcac gtcatcgctg
      301 tcctcgagcg cgtcgatcag gcggaagatc ttgcgcgcgg tgtcggcgtc gatctccacc
      361 ttgaggttcg ggacgaactc gacgtcggcg gactcgtagt cgatgcccgc ctcctgcagt
      421 gcggagcgca ccggcacgag gtcgctggcg tcggtgatca cctggaagcc ctcggcgtgc
      481 tcctcgatct cctcggcgcc ggcctcgaga gcggccatca tcacgtcgtc ctcggtggtg
      541 ccctcggagc cgaccacgat gacgcccttg cgggtgaagt tgtaggcgac cgaccccggg
      601 tcggccagcg tgccgccgtt gcggctcaga gccgtgcgga cctccgcggc tgcacggttc
      661 ttgttgtcgg tcagacactc gatcatcagg gccacaccac cctgcccgta gccctcgtac
      721 atgatcgagg tgtactcgac ggcctcgccg ccgatgcccg caccgcgctt gatggcgcgg
      781 tcgatgttgt ccttcgggac ggacgtcttc ttggccttga gcacggcgtc gaagagcgtc
      841 ggattgccgt cgagatcggc gccgccgagc ttggccgcga cctcgatgtt cttgatgagc
      901 ttggcccacg acttggcacg gcgcgcgtcg atgacggcct tcttgtgctt ggtcgtggcc
      961 cacttggaat gcccggacat atatctccgc tcgtctcgtg tgcccggagt cgcccccagg
     1021 gcatcgtcca ttctaacgaa tcagggacgt tccgccgtca gccgttcagc cgggtgccgc
     1081 cggtgcgccc tcgtgcctct gcgacggcgg cgctctcagg cagggcgatc ggctcgtcca
     1141 tcgcggcgtc cggcgtccag tgtgcccagc cctcgtcgag cggccatcct cctggcatca
     1201 ggaactccgc agccgcgtgg tcggcgatcg cccgcacggc gtccgcctgc gcagcggtga
     1261 atcgaccctg tgcgacggcc gcggcgagtt cgtcggcatc cttcagccac acgtcctcct
     1321 cacccggatg atcggcttcg atccagaggt cgaggatgag gtcacgcgag aagatgccgg
     1381 gtgcgccggt agcggagcgc aggtgaggca gctccagatt cacgtacgcg ccttcgggcg
     1441 tcccatccgc tccgcggaag aaccacaccg accaggggcg gccactcggt gccacgcgca
     1501 ccacgccggg cccccgccag gtcgactcgc ggatctccca tggcacgctg aaccgctcct
     1561 cgagcgggac ctctctggtg gccctgccgt cagccggcac ggattccagc cgggcggatc
     1621 cggacgggat ccagaccacc aggccgcgtg cgtcgtcccg gatcacccgc gcggtctcga
     1681 tgtaccgtcc gtagcgccac aggatgggtt cgccggcggt gaagcgcggt gcatcttcgg
     1741 agtccgcggg cacagcatcc gcgaactcgt cgccggcatg agcgccctca tgctgatcga
     1801 cgagcaactc gacagcctcg tcgagcggga tgctccgcgc acacagcgcc tcccacgcct
     1861 ctcccctcct gccgacgagg gtgacggttc ccccgctggc gctgacatcc gtggatccga
     1921 ggggaaggcg catgcggcca cgctaccgtc cgcgtcggcg aacgcgttca ggcagggtgc
     1981 ccattgcgac ccctgtcgcc gacggtgacc tccggctagg ctccacggat ggacatgctc
     2041 aagggcgagc agatcgccgc cgctgcactg accgactggc gcaagctcgc ccagggactg
     2101 tacgcccgat atctcgtgag ggactaccga tccgcagccc gattcgtcac ggcgatcgcc
     2161 gaagccggtg cgcggatcgg gcaccacccg cgcaccacga tcggcgaagg acacgtcgat
     2221 ctgaggctcg tcagcgtgga cgccgtctac cgtgacggcg agggcgtcga gcacgtcgtg
     2281 gaatgggtga cgcagaacga tgtggacctc gcacgcgcga tcaccgagat cgccgccggg
     2341 cagggcatcg cgcccgaacc caccgccgtg tccgagatcg agctgggcct cgacaccccc
     2401 gactcgggca tgatcgcccc ggtgtgggcc gcgctgctga cggggcacgc cgaatcccgg
     2461 ggacgcggta cgcccagcga cgagatcagg gatgccacag gacgcgtgcc gcatctgtgg
     2521 ttcggtgatg ccgacgacgc gtcgccgcag cggttccatg tcgaggtgta cgtgccgccg
     2581 gagtgcgtcg cggagcgcgt ggcagctgtg aaggacgcgg gcggtgtggt catcgacgac
     2641 tcggatgctc cgggactcac cgtgttcgcc gatcaggacg ggaaccgcgg cgtgctgtgc
     2701 gcggacacgt ctgccgcgag ccgtccggcg ggcgacgccg gcgcagtccg caccgcctga
     2761 tccggctcag ccgcgcgcgg cgacccggtc caggaaccgc tggtggaacc gcggctcgcc
     2821 gctcagctcc ggatgaaagc tgatgccgag caggttcccc tgctcgacgg ccacgacgcg
     2881 tccgtcctcc agcgccgcca gggtggtggc atccgcgccg acctcctcca ccacggggcc
     2941 acggatgaac gtcgtgtgca ccggatcggt cccgagcgcg gggatgtcga gctccgcctc
     3001 gaaggactcc gtctggcggc cgaaggcgtt gcgcctggtc agcacgtcca tcccgccgaa
     3061 ggtctgctgg ccgtcgatcg cgtcgatcag acggtccgag agcatgatca gccccgcgca
     3121 ggtgccgagc atcggcatcc cgtcgcggat cgccgcacgg atcggctccg ccagaccgaa
     3181 ctgccgggac agcttgtcga tcaccgtcga ctcgccgccg gggatggcca ggccgtcgac
     3241 ctgggcgaac tcctcgggac ggcgcacgcg cacgacgtcg gcaccgaggt gctcgagcag
     3301 ggcgacgtgc tcgcgcacat cgccctgcag cgcgagcacg ccgacgcgcg ccgtggtcac
     3361 cagccccgct cggcgagacg gtgcggcgcg ggcaggtcgg cgacgttgat gcccaccatg
     3421 gcctcaccga ggccacggga gacctctgcg atcaccttgg ggtcgtcgta gaacgtcgtc
     3481 gccttgacga tggccttcgc gcgggcgaca gggtcgccgg acttgaagat gcccgagccg
     3541 acgaacacgc cgtcggcgcc cagctgcatc atcatcgcgg catccgcggg ggtggcgacg
     3601 ccgccggcca cgaacaggac gaccgggagc ttgccggtct cggcgatctc ggagaccagc
     3661 tcgtatggcg cctgcagctc cttggccgcg acgtacagct cgtccttcga cagcgcaccg
     3721 agggctgcga tctctccgcg gatcttgcgg atgtgcttca tggcctcgga gacgtcgccg
     3781 gtgccggcct cgcccttgga acggatcatc gccgcgccct cgttgatgcg ccgcagcgcc
     3841 tcgcccaggt tggtggcacc gcagacgaag ggaaccgtga agccccactt gtcgatgtgg
     3901 ttgacgtagt cggccggcga cagcacctcg gactcgtcga tgtagtcgac gccgagttcc
     3961 tgcagcacct gcgcctcgac gaagtggccg atgcgggcct tcgccatgac cgggatggag
     4021 accgcgtcga tgatgctgtc gatcatgtcg gggtcgctca tgcgcgagac gccgccctgg
     4081 gcgcggatgt cggcggggac ccgctcgagc gccatgaccg cgacggcccc ggcatcctcg
     4141 gcgatcttgg cctggtcggc ggtgacgacg tccatgatga cgccgccctt cagcatctcg
     4201 gcgagtccgc gcttgacgcg ctgcgagccg gtggtggggg tctggtctgc catcgtgtct
     4261 cctcgggaag aaggtcagta caaaagattc tttggcctat gtcaaaagat agcaccatag
     4321 aatcggcgtg tgagcatgga tatcgccggg acgaccgctg cggagatcgc cgacagcgtg
     4381 cgggcgctgc gcgagcgcgg gatgctgcgc ccgggcgacg cgctcccccc ggtgcgggag
     4441 ctcgccggcc ttctc
//
LOCUS       DATMSX010000314         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_14223,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000314 DATMSX010000000
VERSION     DATMSX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14223"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..936)
                     /locus_tag="VN041_09555"
     CDS             complement(106..936)
                     /locus_tag="VN041_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29320.1"
                     /translation="MSALAPTATRIIPASERRLKNHAGLGLALRNTVTMAGRGLIKIR
                     RTPEQLIDVTLQPILFTLMFTYIFGGAISGDVQSYLPVIIPGILVQTVITTSVVTGVQ
                     LREDMDKGVFDRFRSLPIARIAPLSGALLADTVRYAIATVLTFTMGALMGFRPGGGIA
                     SVITAGLLVILCSWAVSWIFAFFGVIARTASSVQGISMLILFPLTFLSNAFVPVDTMP
                     GWLQWFVNINPVSHLVTAVRDLTATGAFGADAWISILGAAVIVAIFAPLTVRAYMRKA
                     "
     gene            complement(933..1943)
                     /locus_tag="VN041_09560"
     CDS             complement(933..1943)
                     /locus_tag="VN041_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU29321.1"
                     /translation="MTNSTSEWAIAADGLVKVFGANRAVDGVDLRVPTGTVYGVLGPN
                     GAGKTTTINMLATLMRPDGGDARIFGHDVVREAHIVRQLIGVTGQFASVDETLSANEN
                     LMIFARLLGLTRAEARAKSSDLLERFGLTEAARRPLAQFSGGMRRRLDLAASLIAQPP
                     LIFLDEPTTGLDPRTRGQMWDTIRELVRTGSTVMLTTQYLDEADQLADRIAVIDRGRV
                     VAEGSPDDLKASVGASSLQLRLTEPADAEDALRAIERVLGVQGVLSPEAARITAPMTD
                     ADRVADLLIVLREAGIGLNEMSVQKPTLDEVFLTITGHSAEADTDDTAAPAASQKEEV
                     RA"
     gene            2054..2497
                     /locus_tag="VN041_09565"
     CDS             2054..2497
                     /locus_tag="VN041_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29322.1"
                     /translation="MSSRIGVAVMGAALFLYVVVAAWFAVLMFSAATPLSIAFGVGLI
                     IIAVIGGWALVRELMFGFSADRLGRQLDGEGGMPEAPEALSPSGRLLREDVEPMIAQY
                     TSAAAADADDWRARYRLGVVQEASGRRKDARASIREAIRLSRVRP"
     gene            complement(2522..2947)
                     /locus_tag="VN041_09570"
     CDS             complement(2522..2947)
                     /locus_tag="VN041_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HWU29323.1"
                     /translation="MPVTSEAAATWTGSLTEGSGTVAFSSSNLGTFPIDWKARSEGSD
                     TTTTPEEMIAAAHASCFSMALSHALTENGTPPERVNTSASVTFKPGTGITGSHLNVNA
                     TVPGLTPEKFQEIAEGAKTGCPVSQALAGIEITLEASLA"
     gene            complement(3035..>3251)
                     /locus_tag="VN041_09575"
     CDS             complement(3035..>3251)
                     /locus_tag="VN041_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HWU29324.1"
                     /translation="RPPRFAQGVGLFVVGVGLLLHVLGVPLALPIATAMAFVAAFLNA
                     AFGFCLGCELYLLLQRAGVLGRGPRAA"
BASE COUNT          482 a         1032 c         1235 g          502 t
ORIGIN      
        1 tgacggtgct cgcggggcgc tgatcgcgtc gagccgacgg cacgcgggac cgcggagcgc
       61 agcagaggga cgcccgccgg cggcggacgt ccctctggtg tcgtgtcagg ccttgcgcat
      121 gtatgcccgc acggtgagcg gtgcgaagat cgcgacgatc acggctgcgc cgaggatcga
      181 gatccaggcg tccgcaccga atgcaccggt cgccgtgagg tcgcggacgg ccgtcaccag
      241 gtgcgagacc gggttgatgt tgacgaacca ctgcagccac ccgggcatgg tgtcgaccgg
      301 gacgaacgcg ttcgagagga atgtcagcgg gaacaggatg agcatcgaga tgccctgcac
      361 gctcgatgcg gtgcgcgcga tcaccccgaa gaacgcgaag atccagctga ccgcccagga
      421 gcacaggatc acgagcagtc ctgccgtgat caccgacgcg atgcctccgc cgggacggaa
      481 gcccatcagc gcacccatgg tgaacgtgag cacggtggcg atcgcatagc ggactgtgtc
      541 ggccagcagc gcgccggaca gcggggcgat gcgcgcgatc ggcagggacc ggaaccggtc
      601 gaagacgccc ttgtccatgt cctcgcggag ctgcacgccg gtgacgaccg aggtggtgat
      661 cacggtctgc accaggatgc cggggatgat caccggcagg taggactgca cgtcccctga
      721 gatcgcgccg ccgaagatgt aggtgaacat cagggtgaac aggatcggct gcagcgtgac
      781 gtcgatcagc tgctcgggcg tgcggcggat cttgatcaga ccgcgcccgg ccatcgtgac
      841 ggtgttgcgc agcgcgaggc cgagaccggc gtggttcttg agccggcgtt cggacgccgg
      901 gatgatgcgg gtggcggtgg gggcgagagc gctcatgcgc gcacctcctc cttctgtgag
      961 gcggccgggg ccgcggtgtc gtcggtgtcc gcctcagcgg agtgaccggt gatggtgagg
     1021 aagacctcgt cgagggtcgg cttctgcacg ctcatctcgt tcagcccgat gccggcctcg
     1081 cggagcacga tgagcaggtc cgcgacccga tcggcgtcgg tcatcggagc ggtgatgcgg
     1141 gcggcctcgg gtgagagcac gccctgcacg ccgagcacgc gctcgatcgc gcgcagcgcg
     1201 tcctcggcgt cggcgggctc cgtgagccgc agctgcagcg aggatgctcc gacggatgcc
     1261 ttgaggtcgt ccggcgagcc ctcggcgacg acgcggccgc ggtcgatcac ggcgatgcgg
     1321 tcggccagct gatcggcctc atcgaggtac tgcgtcgtca gcatcacggt ggacccggtg
     1381 cggaccagct cgcggatggt gtcccacatc tgtccgcgtg tccgcgggtc cagtccggtg
     1441 gtcggctcgt cgaggaagat gagcggcggc tgggcgatga gtgaggcggc gaggtcgagg
     1501 cgtcggcgca tgccgccgga gaactgcgcg agcggacggc gcgccgcctc ggtgagcccg
     1561 aaccgctcga gcagatccga ggacttggcg cgggcctcgg cgcgggtcag cccgagcagc
     1621 ctcgcgaaga tcatcaggtt ctcgttcgcc gacagcgtct cgtcgaccga ggcgaactgg
     1681 ccggtcactc cgatgagctg gcggacgatg tgcgcctcgc ggacgacgtc gtggccgaag
     1741 atgcgggcgt ctccgccgtc cggacgcatc agcgtcgcga gcatgttgat ggtggtggtc
     1801 ttgccggcgc cgttcgggcc gaggacgccg tagacggtgc cggtgggaac gcgcaggtcg
     1861 acgccgtcga ctgcgcggtt cgcaccgaac accttcacga gtccgtcggc cgcgatggcc
     1921 cattctgagg tggagttcgt catgtgacga gcatccgtcc ggccgctgtc gtcgcactgt
     1981 cggttcgctg tcggcgactg tcagtcgctg tcagcgggaa ggtgtcgccc tcgcgcgctc
     2041 ctaggattga gacatgagct ctcgcatcgg cgtcgcagtc atgggcgcgg cgctcttcct
     2101 ctacgtcgtc gtcgccgcct ggttcgccgt gctcatgttc tccgccgcga ccccgctgtc
     2161 gatcgcgttc ggggtgggcc tgatcatcat cgccgtgatc ggcggatggg cgctcgtgcg
     2221 cgagctgatg ttcggattct ccgccgaccg gctcgggcgt cagctcgacg gggagggtgg
     2281 gatgcccgag gcgccggagg ccctgagccc gagcggacgc cttctccgag aggacgtcga
     2341 gccgatgatc gcgcagtaca cgtcggcggc cgccgcggac gccgacgact ggcgggcgcg
     2401 gtaccggctc ggtgtcgtgc aggaggcatc cggacgcaga aaggacgcca gggcgagcat
     2461 ccgcgaggcg atccgtctct ctcgcgtccg tccctgacgt cccgcaggac cgccgaggcg
     2521 atcaggccag gctggcctcg agggtgatct cgatgccggc gagggcctgc gacaccgggc
     2581 aaccggtctt ggcgccctcg gcgatctcct ggaacttctc cggggtcagg ccggggacgg
     2641 tggcgttcac gttcaggtgg ctgccggtga tgccggtgcc tggcttgaag gtgacggatg
     2701 ccgaggtgtt cacgcgctcg ggcggggtgc cgttctcggt cagtgcgtgg gagagcgcca
     2761 tgctgaagca ggaggcgtgc gcggcggcga tcatctcctc gggtgtggtg gtcgtgtcgg
     2821 agccctcgct gcgggccttc cagtcgatcg ggaaggtgcc gaggttcgag ctcgagaacg
     2881 cgacggtgcc ggatccctcg gtcagggaac cggtccaggt ggcggcggct tcgctggtga
     2941 cgggcatgtt tcctccaggg gtcgcgatca tgcggtggac gcgggttcgc gtcgcttcgc
     3001 aagcctatcc aggaggcggc tccggcggta ccggtcaggc cgcgcgggga ccgcgtccga
     3061 gcacgccggc gcgctgcagc agcagataga gctcgcagcc gaggcagaag ccgaacgccg
     3121 cgttcaggaa ggcggcgacg aacgccatgg ccgtcgcgat cggcagcgcg agcggaacgc
     3181 ccagcacgtg cagcagcagc ccgacgccga ccacgaacag gccgacgccc tgcgcgaagc
     3241 gcggcggtcg g
//
LOCUS       DATMSX010000315        22310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_109044,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000315 DATMSX010000000
VERSION     DATMSX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 22310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22310
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109044"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VN041_09580"
     CDS             complement(<1..291)
                     /locus_tag="VN041_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29325.1"
                     /translation="MSHEPLLLGIDAGGTSTRAVLTDLHGECLGYGVGGRGNPISAGP
                     ERAADGVLAAVGAALLPSGRTLADIAVIVPAMAGMRAAGGADWLLDRLTPQGF"
     gene            382..1314
                     /locus_tag="VN041_09585"
     CDS             382..1314
                     /locus_tag="VN041_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HWU29326.1"
                     /translation="MPRTKDFGNGMTIARRIAAGRHTMPTAMSKIADVVTGHPAAPVE
                     LTITELADRAGTSPATVTRFCRAIGFDGYTQFRVGVASEIGRGDADESWQADIGEEFG
                     ATDEPGKVLQTLVALHVASLESTAARLDLDAVMQVANAIAASRHVDIYGVGGSGVIAR
                     EFQGRLYRIGVNAHAWSDVHDGLTSAVMQNASSVAIGVSSTGRTDETVQMLSQAGAAG
                     AFTVAITHDADSWLAGIADISLATAEPSHYLRPDDLSVKHSQLLVLDLLYLLVAQQMF
                     GEASTHLAASAMAVSAHRRPVRVPKPAPVTEENR"
     gene            1311..2063
                     /locus_tag="VN041_09590"
     CDS             1311..2063
                     /locus_tag="VN041_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWU29327.1"
                     /translation="MTATPTDLLQQATSRLERLAADAEAGALDAAIALMVKSLDDGGV
                     IHAFGTGHSEAFAMEIAGRAGGLIPTNRFALRDIVMHGDRDVEVLTGSLEREPWVVDE
                     LMATVPVGEGDVFLIASNSGVNGSIVGTALWAQEHGHKVIAVTSLQHTARVEPKHPSG
                     KRLSEIADVVIDNLAPYGDSTLEITDGIGAGAISSITAAFIAQLLTLGVARTIAERGE
                     TPPMYISANIPGGDEHNSVLEDRYAGRLRRGA"
     gene            2147..3565
                     /gene="ngcE"
                     /locus_tag="VN041_09595"
     CDS             2147..3565
                     /gene="ngcE"
                     /locus_tag="VN041_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0015764 - N-acetylglucosamine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine/diacetylchitobiose ABC
                     transporter substrate-binding protein"
                     /protein_id="HWU29328.1"
                     /translation="MELKEASISRRNLLRGAATAALLLPFGATLASCAAPGGGGGGGA
                     GTGKVTADNPFGVAANSKVDAVIFNGGYGIDYVETSAKIMEGNKKLGGVSVKVNSSTK
                     IAQELQPRFVGGNPPDLIDNSGANSIGWNTILDQLETLDDVLDSNNLEGTKISDTLFG
                     GVKAPGTFDGKFVALNYVMTVYGIWYSSSLFEENGWTVPTSWQDLKALGEAAKAKGKY
                     LFLWGKEAATYYQTMVVDSAVIQAGDDVRLPLENLDAKAWSHPAIQGILEILYDLIKS
                     GYVKPGGGGTQFTQAQSQWSLDQEALLYPSGSWIENEMKKTTKDGFKMKGIAELPLDA
                     NQTTPKGTMRAEAGEPFIVPKKAKNPAGGKELLRTMLSKESATAFAKAKLAPTIVKDT
                     VPADGFGSTALVSQSELLAAAGDNVFTIKSFNLYGMNSDQLPIWNSFLGGGMTVAEIT
                     SQLQALTDKVREDSSVKKIEIK"
     gene            3637..4584
                     /locus_tag="VN041_09600"
     CDS             3637..4584
                     /locus_tag="VN041_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU29329.1"
                     /translation="MSPRLGGRKLTFDFISFIAVFLLLPLAIFLIFVISPFVQALYYA
                     MTNWTGFSPNMDFVGLDNYVRLFNDSTFKQAMLNNVVLALIVPLITIVIALVFASMIT
                     VGGPSHGQVRGLRGSSFYRVISFFPYVIPAIVIAILWSMIYAPTGLLNGALGALGIDT
                     GQFAWLGDANTAMGATIFVIVWSMVGFYMILFIAAIKGIPAETLEAARIDGAGRFRTV
                     TSILLPQMRDNVQTAYIYLGIMALDAFVYMAGLNSTGGPGNSTLVMSQYLFRTAFEKG
                     QFGLATAMGVVLAVLTLLFAALIIGVFRLIGGKDEGGRA"
     gene            4581..5489
                     /locus_tag="VN041_09605"
     CDS             4581..5489
                     /locus_tag="VN041_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU29330.1"
                     /translation="MSTDTRAAITIDRHSASGKDVRTTKSTAGDKVVGGVSHVVMVIW
                     SLIVILPMLWTLIGSFKTTKEIFKSPFGLPANWNFDNYVSAWVDNNFGGMFLNTVIVV
                     GVSLVLVMVLGAMCAYVLARFSLPGSKVIYYLMLAGLTFPVFLAIVPLFMILQNMQLL
                     NTLAGLIVTYVAFALPFTVFFLFSFFKSLPYEIQEAAYVDGASEWRTFFQVMLPMAKP
                     GMAAVAIMNFLGLWNQFLLPISLNADKDNYVLSQGMAAYASSAGYALDFGQMFAAVMI
                     TIIPVLIVYILFQRQLQGSVSQGTSK"
     gene            5595..6389
                     /locus_tag="VN041_09610"
     CDS             5595..6389
                     /locus_tag="VN041_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU29331.1"
                     /translation="MTTRPPTRRRRTALVVVGALAAALTVFLGISRPWMPPAETPPIA
                     ASESGVQPAPLALPEHPKVLVFGDSWTYGSAATPPTNGYAYLLAGLIDGTTVVDGVRG
                     SGYQKPGLDGPDYLTRIKALDPSIAPDLIVIEGSINDRRENAAAFPAAVNAAWDALTG
                     KFPDVPIVVLGPAPHELPVGASTARIDRDLSRLAAQRAWWYISPVQENWITPENYLRL
                     IDVGAGRRHPTNAGHEYLAEKVHDALERFSTAPVTAADGAHQKPAK"
     gene            complement(6349..6924)
                     /locus_tag="VN041_09615"
     CDS             complement(6349..6924)
                     /locus_tag="VN041_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWU29332.1"
                     /translation="MTERTILTVGHSTHPLDEFLGLLSDADIGGIVDVRRLPGSRRFP
                     WFDQDALSSSLADAGIRYTRIPELTGRRPVQHDVPDEVDALWRNRSFHNYADHALGPD
                     FARGLDELVRLGGESRSAVMCSEAVWWRCHRRIIADHLLARGIRVEHIMPDGRISPAE
                     LTPGAVIRGTQVVYPAPGPTSPASDGRRPPQ"
     gene            complement(6917..7132)
                     /locus_tag="VN041_09620"
     CDS             complement(6917..7132)
                     /locus_tag="VN041_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HWU29333.1"
                     /translation="MPHRFKTGDHVTWNSEAGHVSGRIVKVHTADFDYKGHTHRASEA
                     DPQYEIKSDKTDHIAAHKGGALKLADD"
     gene            complement(7607..7765)
                     /locus_tag="VN041_09625"
     CDS             complement(7607..7765)
                     /locus_tag="VN041_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29334.1"
                     /translation="MVSSPQKTFPRLDRIEHAELLAMTAAPSILPASSCESFTPGVLV
                     MRQAARDA"
     gene            7764..8633
                     /locus_tag="VN041_09630"
     CDS             7764..8633
                     /locus_tag="VN041_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWU29335.1"
                     /translation="MTDTDLLDAFADYQRAGNRSPRTIRTRTSILRCFARRQDTTLLD
                     ATVFHLRREVGRDELAASSRASVRAVFLAFYGFLQSEGIRTDNPALRLPVVKVPPHRP
                     RPYTAEQIDAMLSSGAYYRTRVMILLGAYQGLRASEIARVHADDVDLQAGTLKVLGKG
                     GRIDYLPLHDVTRELAPTMGGGWWFPARHGGTGHIKGSSVSDLLHDARERAGIVDDRL
                     TGHSLRHSFGTELVRRGANIRAVQELMRHSSLSTTQRYTQVLDDDRRAALAVLPAPAI
                     PARSGRGGDRLAA"
     gene            8630..8809
                     /locus_tag="VN041_09635"
     CDS             8630..8809
                     /locus_tag="VN041_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29336.1"
                     /translation="MTDAAYIITTILSTLLGLLALYFVIKLAVRHALAEHRLAEARIA
                     DELRRAEASRAARSS"
     gene            complement(8811..9173)
                     /locus_tag="VN041_09640"
     CDS             complement(8811..9173)
                     /locus_tag="VN041_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29337.1"
                     /translation="MNKTLAFILIAALAVVGLAGVVVLLVLRPEAVDTLTNLLMQLLI
                     MLGGFGGLAAIQSRQGEEIKTIKHNTNGTLSAKDAEIANLRAALAEHAPQALVTVDTA
                     STPTVTRAQLREDGKPGY"
     gene            complement(9170..9958)
                     /locus_tag="VN041_09645"
     CDS             complement(9170..9958)
                     /locus_tag="VN041_09645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWU29338.1"
                     /translation="MRDRLPMPFSNPSTYRGHSGVDFGQRGGTPIFASGPGVVARHST
                     SPSGGHWIVIRYDGIGEVGYAHLNDWTGCPPVRSRVDLGDLIGYVGWDGHVVPPGRAG
                     AHVHVTKISNQTYAATAALFDMTRWVGMPAPTGKPKPLPTPKPPTPSTPAPEPEEDED
                     DMPIIIRNTENKEVSLLDPDIGTDLPRVVAGQAAKHRTEKTTNGVVNTYRGFLVTTDK
                     AIAEAWGRSYARSHGNAPQDRTPADYVTAQQEASRLATERNPRA"
     gene            complement(9955..11571)
                     /locus_tag="VN041_09650"
     CDS             complement(9955..11571)
                     /locus_tag="VN041_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29339.1"
                     /translation="MTVTEHYAHAQSDALPGVELGVEGGEVGLAVSSWPHVQATIRTP
                     ARAGLYAAMDPRTAAHRVVLSCGEQVMGLHREFDLGIRHVGIGADGLLEVQLASDEAI
                     VEDETPDADVRPWAYLRTNGYVAEVLTEMVLARSGFALTDPGPVADITPYWSLTNRVV
                     NPRFQTTTKGAAAGSGTSGLVSATHTAAGSTARRALQFTCGTAGGSAIVAGWDPVAGH
                     ESALPVSAGDWVSASFDASCVAPNRDRKIRVQFFDGAFTLIYEVIPGGQPTAPDTRWT
                     THAAVVQAPIGAVYCAVRAFLGPIAAGADQRVFVTNVSVVQWDDYVPAFNGGDTPPGG
                     YTVSWDGAPDDSTSTRYPIAPLDPESLVQKAGQGGMSFLAPVLQACGLRLVCDETRTW
                     SLRGIDHVEPGELTIAYGETMTDFTLAAGLDGWCDSATVHYEWERYGVTYEMDDTFDL
                     VTPPRKRIRRTLNMPYPGPGRAENIVRRAQAVGGRSSTAKAVSNWSVKAEQTLTVTTD
                     DTTLTGQVGAVTFDLDTDEMTVTIREEPPA"
     gene            complement(11568..11930)
                     /locus_tag="VN041_09655"
     CDS             complement(11568..11930)
                     /locus_tag="VN041_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29340.1"
                     /translation="MVTTITHSSGIIVPDIMDGFDAARDAGTLIHPVLGSPDPDVSLR
                     PAGRRSGELRLVFSSAAGAVTAETVLAVPQVLALSDPDVGAVAMSFVVAGGQLRVEID
                     DETRSAWVLSVPFQEVSP"
     gene            complement(11930..13174)
                     /locus_tag="VN041_09660"
     CDS             complement(11930..13174)
                     /locus_tag="VN041_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29341.1"
                     /translation="MAKGIDLSIAADTRSAMSAIQRGIIDPLEDVTDLLERTGRESKD
                     AGNDLERSMRDAQRRTEDSKDEIRELRDELNKAGRAGKKAGDDIGDGVRAGGDGFARV
                     KEGAQEVTQEIGQNLGEAVSSVRGNLADLGQVGQDTLGGLAATLSGTGPAGIAGAAVL
                     AAGAVGLGLVTAELEKQQEEAAKFRERISDAYRGAAEDGRTFIDDQTKISAALDILFD
                     PERAEDLKTAQKDAITLGLDLSKILAAQTGDTAALAEVQTAVAAVQQKALANENLAER
                     WDKDLTALYGRWYDIGQVTDENARKAVTARQITSDYLLDAISKAQDATEEVDEFGNRL
                     IHLPEGRDVVIDAKTGQASENLAKFRGDADGVIDHVSGRKAVIKVAADTSDADRALTR
                     FINKGRQIKIGSKIVGPAGEWD"
     gene            complement(13167..13682)
                     /locus_tag="VN041_09665"
     CDS             complement(13167..13682)
                     /locus_tag="VN041_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29342.1"
                     /translation="MLRVSALSSRELLTVLRGLRTLDRDTKRHLRQSLKDMVLTAWQA
                     ELAKHASTSLESRVLVSTGRARVSDQNVRLTSASLSRSLSGGLKPSESYGPVEFGANP
                     RPGAKEEAHSRKGTPFTRYRDPNRPFKKPNRRGYVVYPSAASIIPRVLAMYVQTFVRA
                     IHEALEGKSNG"
     gene            complement(13698..14108)
                     /locus_tag="VN041_09670"
     CDS             complement(13698..14108)
                     /locus_tag="VN041_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29343.1"
                     /translation="MAFNPLYMTESVLQLGTAPTDFAAEISGAQLVPTSSSATWKGLK
                     PGSVFTAAGLATWALTVNLGQDHETSTSLSNYLFDHEGETVPFTLEPIAGGTGFAGTI
                     IVQAATIGADVDQFGTASVTMPAKGKPTRTLAGA"
     gene            complement(14111..14497)
                     /locus_tag="VN041_09675"
     CDS             complement(14111..14497)
                     /locus_tag="VN041_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29344.1"
                     /translation="MTAFGDLRAQLENDLLANIEPGKFRWVPAQTRLDRPAKPTLMLK
                     LSTVSRHPGAQQVLMPSFVVTVVSEHADQVQADIDLDGLLDELIAAIQPIRWVAFRSA
                     EKAQFQSKYLCYDIQLEALTTIKKEA"
     gene            complement(14494..14853)
                     /locus_tag="VN041_09680"
     CDS             complement(14494..14853)
                     /locus_tag="VN041_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29345.1"
                     /translation="MAVDWITPDDLPALWPDSEGVPSEMLQPMLDAAQDACEAWVPAE
                     GDPERPRDATIRLGQLTLVRGLWNDALAGEDGLLGADGFDYNPPWLAREARRMMRPQR
                     GGNSLATGHDEFPEVIA"
     gene            complement(14928..16508)
                     /locus_tag="VN041_09685"
     CDS             complement(14928..16508)
                     /locus_tag="VN041_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29346.1"
                     /translation="MTETGLFQRIDGTRRVRGLLLPFGELSRPNLSGNEPVMFSADSI
                     TLPRDPSVVTLNRKHDRFDPIGRAVALEKTPAGVVAEFEIAETDEGDSFLASPLRKLS
                     AEVAELVRAGANAVRSKLTGAAAVDEGAFASAALFAVADGVIGEDHTETVYTDENGRE
                     YRRVYDSTTEQTTTDAGVTTITTTTITETTDPAEPDEGEADMANAQVPSTLLASKAEK
                     DKADPITSATFSAMIANAIRQPENRETMLAALNDVKIDGANAVGTGAIPPEFIGEIWS
                     GIPFVRRVIPLLQHGDLTSLSATGWRWTTEPEVAEWAGNKAAVPSNTPATEPATWGFQ
                     RFAGGWDIAREFIDFGETEVINSFLTAAAGSYAKKSDAWVLTKLLAAGTAMNIAAAVP
                     ADVPAALVGIVDGALALIAADARPSYALVNPADYRPLLFTKKSDVLEYLNLALGLEDG
                     DLSGFKIVPHTGIPAGTVTVGAREAAAVHEMPGSPIRVNALDMVKGGVDEALFGYVQF
                     RDYYAKGIQNVTLTPAGA"
     gene            complement(16514..17581)
                     /locus_tag="VN041_09690"
     CDS             complement(16514..17581)
                     /locus_tag="VN041_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29347.1"
                     /translation="MKFSRRLSAIQDIESGTLLKMPGVDAAHADGIVSPWSDGQLNSF
                     VWADIYGLNVVPVTRKEAMSVPAVAASRHRIVQLADRPLRALRGAEDVTQSQSWLYRT
                     NTAVTPWQRIASTLDDWIFYGDSLWVLERGAIPNGSKYGPILDAVHAPYDRWRVDADT
                     REILVEEQPVPAESVIYLPGPFEGLLNVASETVRAAKALERAWSSRVRNPAPTVILEE
                     REDGTVSKKEANNYTKAVATALRDPDGAVFFVPQKINLRLESGGTIDVLDKARNAVRI
                     DIANLLNLNASALDGAKAQSSLTYETQETERAELQDRLSFWTAPLEHRLSMDDVVPRG
                     TRIRFDFATTTPAQTGTPTED"
     gene            complement(18112..18537)
                     /locus_tag="VN041_09695"
     CDS             complement(18112..18537)
                     /locus_tag="VN041_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29348.1"
                     /translation="MQRLVDEYDGWAIALAPDSLGRYLVECPDDVHVMVWHRRNAPPT
                     GNRVRRVWEPVIIRQPADRRGRGAWPVTNDVLDAPAGRPGFAGAKPAAWTRWVLDALG
                     YAPGDDTLDDLFPGSGAVAAAADGMLALVGADRPDGSKR"
     gene            complement(18680..18925)
                     /locus_tag="VN041_09700"
     CDS             complement(18680..18925)
                     /locus_tag="VN041_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29349.1"
                     /translation="MTLRLRYDGIEGSPREPHKRWYKSPGRDKWSSVDVDGMGIVSVT
                     EEAIHHLLTEAGYTETDPPKWAVMTGTDRPDGSRNDG"
     gene            complement(18922..19164)
                     /locus_tag="VN041_09705"
     CDS             complement(18922..19164)
                     /locus_tag="VN041_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29350.1"
                     /translation="MTATIRREITRDVKGGQVRYVNSWCTDCTYWHAFEFTLDAAYRQ
                     GERHLMNVHDIDATTASGPRRQHEHKTTNRENGSES"
     gene            complement(19161..20876)
                     /locus_tag="VN041_09710"
     CDS             complement(19161..20876)
                     /locus_tag="VN041_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29351.1"
                     /translation="MVSAPVFETASVPPYTGSTSFSLYLWDATRNRGIRPEFLGPLLE
                     DDELRREFLVGARSMGYELVDLDDKETIAAMAAESPKRFPLQPQQLWIVDALNAAGNI
                     DDIDEAVVEAMRRASKTTTIFMWCHGRCLSRANYQVTFSAQSGLKSGARLREWKSRLD
                     RTDPDPEAAAGIPPWKRGIRTTPKAVQRQIALFGEELAPAADTEPLGRRFRIMMGEVG
                     KGIYYTNDSTFLVLKPDADAYRGEAGDISWIDEAQELDPEDGADLLAGIIPLQDTREN
                     SALVVSGTAGEIRSGPLWERIERLRAGGLIAGADYCFPPDTPWESIEREEDALALIQL
                     HHPGVGTLTTIEKQRSNWRKMDKPKWAREYGSLWPETFGERAIPGDWWTKTERGKRPP
                     LPRKVAFGVAVKPGGGVAAIVAAYRDGKRRAVLEVVDHRTGTLWLPKRMQELSKRYPG
                     STIAFDDIAEGKATATEAARLRPKPRMRMQTYRETAAGCIQFLRDLERGSLIHFTGQT
                     GLDVAVERAAKRTTRNDDGVWLFTPMERGDDITCLDAAVRALRNWDQHFDAKSRNDDT
                     GIVAA"
     gene            complement(20870..21166)
                     /locus_tag="VN041_09715"
     CDS             complement(20870..21166)
                     /locus_tag="VN041_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWU29352.1"
                     /translation="MSRHHRASHHTAATHKLRPEIEARLPLPCTECGKPVYRDQKWHV
                     AHLTPASQGGRTTRANTGAAHALCNLRAGGKLGAAATNSGRRRRTDAREGIRSW"
     gene            complement(21258..21572)
                     /locus_tag="VN041_09720"
     CDS             complement(21258..21572)
                     /locus_tag="VN041_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29353.1"
                     /translation="MSTIHDGTVTHLDRIALSHALYETAEQFAELAQRWKPETGIPKD
                     MQRQARLLAQIARAVLGTAYEYTRAEAFYDAGRRILANERAAWEFFRCVQTPPEPQEL
                     NR"
     gene            complement(21569..22141)
                     /locus_tag="VN041_09725"
     CDS             complement(21569..22141)
                     /locus_tag="VN041_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29354.1"
                     /translation="MSVESISIALHHSRATGAAKLVLIGIANHDGDGGAWPSVATLSR
                     YAGVDRRNVQRAVRKLEELHEIRAKIQRGGDSSTADARRPNLYDFVLRCPPDCDGSRH
                     HRTRVSRTGQTEIEELSTRAATAPPGGDSARGGAATAPPEPSYNQPSTTSHKSTHVGN
                     RARGACGHDLIDDRHCSRGCRAADVEGAAA"
BASE COUNT         3393 a         7523 c         7566 g         3828 t
ORIGIN      
        1 gaacccctgc ggagtgagcc ggtccagcaa ccagtccgca ccgcccgcgg cccgcatccc
       61 ggccatcgcc ggcacgatca ccgcgatgtc ggcgagggtg cgaccggacg gcaagagggc
      121 cgcgcccaca gcagccagca caccgtccgc ggcccgctcc ggccctgcgg agatggggtt
      181 cccgcgcccg ccgacgccgt atccgaggca ctcgccatgc aggtcggtca gcaccgccct
      241 ggtcgaggtc ccacctgcat cgattcccag cagcagcggt tcgtgactca tttgtgatgt
      301 tgtcctattt gacgttttcg tcgcgtgaga atagttttca tctcaacgag aaatactccg
      361 ggcgggattc aggaggcaat ggtgccacgg accaaggact tcggcaacgg aatgacgatc
      421 gcgcgccgca tcgcggccgg ccgtcacacc atgccgacgg cgatgagcaa gatcgccgac
      481 gtggtcaccg gtcatcccgc ggccccggtc gagctcacca tcacggagct cgctgaccgt
      541 gccggcacct cccccgcgac cgtcacccgc ttctgccggg cgatcgggtt cgacggctac
      601 acgcagttcc gcgtcggcgt ggcgagcgag atcgggcgcg gcgacgccga cgagagctgg
      661 caggccgaca tcggcgagga gttcggcgcc accgacgagc cgggcaaggt gctccagacg
      721 ctcgtcgccc tgcatgtcgc cagcctggag agcacggctg cacgcctgga cctcgacgcc
      781 gtcatgcagg tcgcgaacgc catcgccgcc tcgcgccacg tcgacatcta cggcgtgggc
      841 ggcagcggag tcatcgcccg cgagttccag ggcaggctgt accgcatcgg cgtcaacgcg
      901 cacgcctggt cggacgtgca cgacggcctc accagtgccg tgatgcagaa cgcctcctcg
      961 gtcgcgatcg gcgtgtcgag cacgggccgc accgacgaga ccgtgcagat gctctcccag
     1021 gcgggcgcgg ccggtgcctt caccgtcgcg atcacccatg acgcggactc gtggctcgcg
     1081 ggcatcgccg acatctcgct cgccaccgcg gagccctcgc actacctgcg tccggacgat
     1141 ctgtcggtca agcactcgca gctgctggtg ctggacctgc tgtacctgct ggtggcccag
     1201 cagatgttcg gcgaggcgtc cacgcatctc gccgccagcg cgatggcggt ctccgcccac
     1261 cgtcgcccgg tgcgcgtccc caagcccgcc cccgtcacgg aggagaaccg atgaccgcca
     1321 ccccgaccga tctgctccag caggcgacct cccggctcga gcgtctcgcc gcggatgccg
     1381 aggcgggcgc actggacgcc gccatcgccc tcatggtgaa gtcgctggac gacggcggag
     1441 tgatccacgc gttcggcacc ggccactccg aggcgttcgc gatggagatc gcgggacgcg
     1501 ccggcgggct gatccccacc aaccgattcg cgctgcgcga catcgtgatg cacggcgacc
     1561 gcgacgtcga ggtgctcacc ggctccctgg agcgcgagcc gtgggtcgtg gacgagctca
     1621 tggccaccgt tccggtcggt gagggcgacg tgttcctgat cgcttccaat tcgggcgtga
     1681 acggatcgat cgtgggcacc gcgctgtggg cgcaggagca cggccacaag gtcatcgccg
     1741 tgacgagcct gcagcacacc gcgcgcgtcg agcccaagca tcccagcggg aagcgtctca
     1801 gcgagatcgc cgacgtcgtc atcgacaacc tcgccccgta cggcgactcc acgctcgaga
     1861 tcacggacgg catcggcgcc ggcgcgatct cctccatcac cgcggccttc atcgcgcagc
     1921 tgctgacgct cggcgtcgcg cgcacgatcg ccgagcgcgg tgagacccca ccgatgtaca
     1981 tctccgccaa catccccggc ggcgatgagc acaactccgt cctcgaggac cgctacgcgg
     2041 gccgcctgcg gcggggagct tgacctaaga gctcaccctg ggaccctgag ctcgtcgaag
     2101 ggcccgaacc ctgtacaccg atcacatcac tacggaaggt atgaagatgg aactcaaaga
     2161 agcatccatc agccgtcgca acctgctgcg cggcgcggcc acggctgctc tcctgctgcc
     2221 gttcggcgca accctcgctt cgtgcgcagc acccggcggc ggcggtggcg gcggcgcggg
     2281 aaccgggaag gtcaccgctg acaacccgtt cggcgtcgct gccaactcga aggtcgacgc
     2341 ggtcatcttc aacggcggct acggcatcga ctacgtcgag acctcggcga agatcatgga
     2401 ggggaacaag aagctcggcg gcgtgtccgt caaggtgaac tcctcgacca agatcgcgca
     2461 ggagctgcag ccccgcttcg tcggcggcaa cccgccggac ctcatcgaca actcgggcgc
     2521 caactcgatc ggatggaaca ccatcctcga ccagctcgag acgctcgacg acgtgctcga
     2581 ctccaacaac ctcgagggca ccaagatcag cgacacgctg ttcggcggcg tcaaggcacc
     2641 cggcaccttc gacggcaagt tcgtggcgct gaactacgtc atgacggtct acggcatctg
     2701 gtactcctcc agcctgttcg aggagaacgg ctggaccgtc cccacctcgt ggcaggacct
     2761 gaaggccctc ggcgaggccg ccaaggccaa gggcaagtac ctcttcctct ggggcaagga
     2821 ggcggcgacc tactaccaga ccatggtggt ggactccgct gtcatccagg ccggcgacga
     2881 cgtgcgcctg ccgctggaga acctcgacgc caaggcgtgg tcgcacccgg ccatccaggg
     2941 catcctcgag atcctctacg acctcatcaa gtcgggctac gtcaagcccg gtggcggcgg
     3001 cacgcagttc acccaggcgc agtctcagtg gagcctggac caggaggcgc tgctgtaccc
     3061 ctccggctcg tggatcgaga acgagatgaa gaagacgacg aaggacggct tcaagatgaa
     3121 gggcatcgcg gagctgcccc tcgacgccaa ccagacgacc cccaagggca ccatgcgcgc
     3181 cgaggccggc gagccgttca tcgtccccaa gaaggcgaag aacccggccg gcggcaagga
     3241 gctgctgcgc acgatgctct cgaaggagtc ggccaccgcg ttcgccaagg cgaagctcgc
     3301 gccgaccatc gtcaaggaca ccgtccccgc cgacggcttc ggctcgacgg cgctcgtctc
     3361 gcagtcggag ctcctggccg cagccggtga caacgtcttc accatcaagt cgttcaacct
     3421 ctacggaatg aactccgacc agctgccgat ctggaactcg ttcctgggtg gcggcatgac
     3481 ggtggccgag atcacctctc agctgcaggc cctgaccgac aaggtccgcg aggacagctc
     3541 ggtcaagaag atcgagatca agtgagcaat tcgacaccca gcggcggacc cggctttcag
     3601 acggccgcgg tcactgcaac ccaggcgggg cgacgcatgt cgccccgcct gggcgggcgc
     3661 aagctgacct tcgatttcat ctcgttcatc gcggtcttcc tgctgctgcc gctggccatc
     3721 ttcctgatct tcgtcatctc gccgttcgtg caggcgctgt actacgccat gacgaactgg
     3781 accggcttct cgccgaacat ggacttcgtc ggcctcgaca actacgtccg gctgttcaac
     3841 gacagcacct tcaagcaggc gatgctcaac aacgtggtgc tggcactcat cgtgcctctc
     3901 atcaccatcg tcatcgccct cgtcttcgcc agcatgatca ccgtcggcgg cccgagccac
     3961 ggtcaggtgc gagggctccg gggttcgagc ttctaccgcg tcatctcgtt cttcccctac
     4021 gtcatccccg cgatcgtcat cgcgatcctg tggagcatga tctacgcgcc gaccggtctg
     4081 ctcaacggcg cgctgggcgc gctcggcatc gacaccggcc agttcgcctg gctcggcgac
     4141 gcgaacaccg ccatgggagc cacgatcttc gtgatcgtct ggagcatggt cgggttctac
     4201 atgatcctgt tcatcgccgc gatcaagggc attcccgcag agaccctcga ggccgcccgc
     4261 atcgacggcg ccggccgctt ccgcaccgtg acctcgatcc tgctccccca gatgcgcgac
     4321 aacgtgcaga ccgcgtacat ctacctcggg atcatggccc tggacgcgtt cgtctacatg
     4381 gcgggcctga actcgaccgg tggcccgggc aacagcacgc tggtcatgag ccagtacctg
     4441 ttccgcaccg cgttcgagaa gggccagttc ggcctcgcca ctgccatggg cgtggtgctc
     4501 gccgtgctca cgctgctgtt cgccgcactc atcatcggcg tgttccgcct gatcggcggc
     4561 aaggatgaag gaggacgcgc atgagcaccg acacccgcgc cgcaatcacg atcgatcgcc
     4621 actcggcctc cggcaaggat gtgcgcacca cgaagtcgac tgccggcgac aaggtcgtcg
     4681 gcggcgtctc ccacgtggtg atggtgatct ggtcgctgat cgtcatcctg ccgatgctct
     4741 ggacgctgat cggctcgttc aagaccacga aggagatctt caagtccccc ttcggcctgc
     4801 ccgcgaactg gaacttcgac aactacgtca gcgcctgggt cgacaacaac ttcggcggga
     4861 tgttcctgaa caccgtgatc gtcgtgggcg tctcgctcgt gctcgtgatg gtgctcggcg
     4921 ccatgtgcgc gtacgtgctg gcacggttct cgctgccggg cagcaaggtc atctactacc
     4981 tgatgctggc aggactgacc ttcccggtct tcctcgccat cgtcccgttg ttcatgatcc
     5041 tgcagaacat gcagctgctg aacacgctgg caggactcat cgtcacctac gtggcgttcg
     5101 cgctgccgtt cacggtgttc ttcctgttct cgttcttcaa gtcgctgccg tacgagatcc
     5161 aggaggccgc ctacgtggac ggcgccagtg agtggcgcac gttcttccag gtgatgctcc
     5221 cgatggcgaa gcccggcatg gccgccgtcg cgatcatgaa cttcctggga ctgtggaacc
     5281 agttcctgct gccgatctcg ctgaacgcgg acaaggacaa ctacgtgctc tcgcagggta
     5341 tggccgcata cgcgtcgtcg gccggctacg ccctcgactt cgggcagatg ttcgccgccg
     5401 tgatgatcac gatcatcccc gtgctgatcg tctacatcct cttccagcgc cagctgcagg
     5461 gatccgtctc gcagggcacc tcgaagtagc atccgtcggc tccgaaggcc cgggttcctc
     5521 aggttcccgg gccttcggcg tcccaccgcg gcatccgaat tctgttctga ccgaacatga
     5581 cttactcttc tccaatgacc acgcgtccgc cgacccgacg gcgccgcacg gccctcgtgg
     5641 tcgtgggcgc gctcgctgcc gcgctcacgg tgttcctcgg gatctcccgg ccctggatgc
     5701 cgcccgcgga gacgccgccg atcgcggcct ccgagagcgg tgtccagccc gccccgctgg
     5761 cgcttcccga gcacccgaag gtgctcgtgt tcggagactc ctggacgtac ggctcggctg
     5821 ccactccccc gacgaacggg tacgcgtatc tgctcgccgg actcatcgac ggaaccaccg
     5881 tggtcgacgg cgtccgcggc agcggatacc agaagcccgg actcgacgga cccgactacc
     5941 tgacccgcat caaggcgctg gatccgagca tcgcccccga cctgatcgtc atcgagggct
     6001 cgatcaacga tcgccgcgag aacgccgcgg cgttccccgc cgccgtgaac gccgcgtggg
     6061 atgcactgac cggcaagttc cccgacgtcc cgatagtcgt gctgggtccc gcgccgcacg
     6121 aactgcccgt cggcgcatcg acggcgcgca tcgaccgcga cctctcgcgt ctggcggccc
     6181 agcgcgcctg gtggtacatc tcgcctgtgc aggagaactg gatcacgccc gagaactacc
     6241 tgcgtctgat cgatgtgggt gcgggtcgaa ggcatccgac gaacgccggc cacgagtacc
     6301 tcgccgagaa ggtgcacgac gcgctggagc gcttcagcac ggcgccggtc actgcggcgg
     6361 acggcgccca tcagaagccg gcgaagtagg gcctggggcc ggatagacga cctgagtccc
     6421 ccggatcacc gcacccgggg tgagctcggc cggcgagatg cgcccgtccg gcatgatgtg
     6481 ctcgacacgg atgccgcggg ccagcaggtg gtccgcgatg atgcggcgat gacagcgcca
     6541 ccacacggcc tccgagcaca tcaccgcgct ccggctctcg ccgccaaggc gcacgagctc
     6601 gtcgagccca cgggcgaagt ccgggccgag cgcgtggtcg gcgtagttgt ggaagctgcg
     6661 gttgcgccag agcgcgtcca cctcgtcggg gacgtcgtgc tgcacgggac ggcgaccggt
     6721 cagctccggg atgcgggtgt accggatgcc ggcatcggca aggctcgagg acagggcgtc
     6781 ctggtcgaac caggggaagc gccgggagcc cggcagccgt cgcacgtcga cgatccctcc
     6841 gatgtccgca tcgctcagca gcccgaggaa ctcgtcgagc ggatgcgtgg agtgcccgac
     6901 ggtgaggatc gtccgctcag tcatcggcga gcttcagggc accgcccttg tgcgccgcga
     6961 tgtggtcggt cttgtcgctc ttgatctcgt actgcggatc ggcctcgctg gcacggtgcg
     7021 tgtgcccctt gtagtcgaaa tcggccgtgt gcaccttgac gatgcgcccg ctgacgtgac
     7081 cggcctcgga gttccaggtg acgtgatccc cggtcttgaa acgatgaggc atgacgggct
     7141 tcctccgatg gcggcgtata gggctattgt gcctcggacg gcccgtcagg tgggtcgcat
     7201 cgggtatcga gacgggagac ggccttcggc cgtcgtgccc gcgtccccat cggccactca
     7261 ccgcgccgcg ggtgggcgag gtcgggtcgc actgcgcacg gatgcggcgc ggcgagcctc
     7321 cggccgatgg ggcccctctg tcggggacgg atagaacgcc gaatgggccg cccggagggc
     7381 ggcccatttc gcgttcgtgc ccccgacagg aatcgaacct gcgacctacg gtaccaacat
     7441 ccgtcggttg gacgactatc gaatggagcg tgcggcatga acttgtcaga cggctatgag
     7501 cgcatcggag cgctcgaaat gggtatcgcg cgatacgtgc tgatcggtga gatcgaatgg
     7561 gcccgtgagc tcgcaatctc actcgcgcag acccgcgaca gcatgctcac gcatcacgag
     7621 ctgcttgccg catcacgagc acgccgggcg tgaacgactc gcacgaggat gccggcagga
     7681 tgctgggcgc ggcggtcatc gcgagcagct cggcgtgctc aatacggtcg agccgaggga
     7741 acgtcttctg aggggaagaa accatgaccg atactgatct gctcgatgct ttcgctgact
     7801 accagcgcgc cgggaaccgc tcgccgcgca cgatccgcac tcgaacgtcg atcctgcgat
     7861 gcttcgcccg caggcaggac acgacgctgc tcgatgcgac cgtgttccac ctgcgccgcg
     7921 aggtcggccg cgacgagctc gcggcatcgt cgcgggcatc cgtgcgcgcc gtgttcctcg
     7981 cgttctacgg cttcctgcaa tccgagggca tccgcaccga caacccggcg ctacgccttc
     8041 cagtggtgaa ggtgccacca caccgaccgc gaccgtacac ggcggagcag atcgacgcga
     8101 tgctctccag cggcgcgtac taccgcacgc gggtgatgat cctgctcggc gcgtaccagg
     8161 ggctcagagc gtcggagatc gcccgtgtgc acgcagatga cgtcgacctg caggcgggca
     8221 cgctcaaggt gctcggcaag ggcgggagga tcgactatct gccgctgcac gacgttacgc
     8281 gcgagctcgc accgacgatg ggcggcggat ggtggttccc ggcgcgccac ggaggcacgg
     8341 ggcacatcaa gggcagttcg gtcagcgacc tactgcacga cgcgcgcgag cgggccggca
     8401 tcgtcgatga tcgactgacc ggacactcgc tgcgtcacag cttcggcacc gaactcgtgc
     8461 gccgcggggc gaacatccgc gccgtccagg aactcatgcg ccattcgtcg ctgtcgacca
     8521 cgcaacgcta cacgcaggtg ctcgacgacg accggcgcgc ggctctcgcc gtgcttcccg
     8581 cacccgcgat ccctgcccgt tcgggaaggg gcggcgatag gctcgccgca tgaccgacgc
     8641 tgcatacatc atcacgacga tcctgagcac tctgctcggg ctcctcgccc tgtacttcgt
     8701 gatcaagctc gccgtcaggc atgctctcgc cgagcatcgg ctcgcggagg cgcggatcgc
     8761 cgacgagctg cgccgcgcgg aagccagtcg cgcggcgcgg tcgtcgtgac tcagtagccg
     8821 ggcttcccgt cctctctgag ctgcgcacgg gtgacggtcg gggtgctggc cgtgtcgacc
     8881 gtcacgagcg cctgcggggc gtgctcggcg agggccgcgc gcaggttggc gatctcggcg
     8941 tccttggcgg agagcgtgcc gttggtgttg tgcttgatgg tcttgatctc ttcgccctgc
     9001 cgcgactgga tcgcggcaag gccgccgaaa ccaccgagca tgatcagcag ctgcatcagc
     9061 aggttggtga gcgtgtcgac ggcctcgggg cggaggacga gcagcaccac gacgccagcg
     9121 aggccgacga cggcgagggc tgcgatcagg atgaatgcga gcgtcttgtt catgcgcggg
     9181 ggttcctctc ggtcgcgagg cgggatgctt cctgctgcgc ggtcacgtag tcagcgggcg
     9241 tccggtcctg tggggcgttg ccgtgggagc gggcgtaaga ccggccccac gcctcggcga
     9301 tcgccttgtc ggtggtgacc aggaacccgc ggtaggtgtt gaccacgccg ttggtcgtct
     9361 tctcggtccg gtgcttggca gcctgcccgg cgacgacgcg cggaagatcg gtaccgatat
     9421 cggggtcgag caggctgacc tccttgttct ctgtgttgcg gatgatgatg ggcatgtcgt
     9481 cctcgtcttc ctccggttcg ggtgccggtg ttgatggtgt gggtggtttc ggggttggga
     9541 gcggcttggg cttgcccgtg ggcgcgggca tcccgaccca acgggtcatg tcgaacagtg
     9601 ccgcggtggc cgcgtatgtc tggttgctga tcttggtgac gtgcacgtgt gcgccggcgc
     9661 ggcctggcgg gaccacgtgc ccgtcccatc cgacgtatcc gatcaggtcg cccagatcga
     9721 cacgggagcg gacgggcggg catccggtcc agtcgttcag gtgcgcatac ccgacttcac
     9781 cgatgccgtc gtagcggatc acgatccagt gaccgcctga cggcgacgtg gagtgccggg
     9841 cgaccacgcc cggcccggaa gcgaagatcg gtgttccacc gcgctgacca aagtccacgc
     9901 cggaatgccc gcggtaggtg ctcgggttgg agaacggcat cggcaagcgg tctctcatgc
     9961 gggcggttcc tctcggatgg tgacggtcat ctcgtcggtg tcgaggtcga aggtgacggc
    10021 gcctacttgc ccggtgaggg tggtgtcgtc ggtcgtgacc gtgagggtct gctcggcctt
    10081 gaccgaccag ttcgatacgg ctttcgcggt ggacgagcgc ccgccgacag cctgtgcccg
    10141 gcggacgatg ttctcggcgc ggccggggcc ggggtagggc atgttgaggg tgcggcggat
    10201 gcgcttgcgg ggtggggtga cgagatcgaa cgtgtcgtcc atctcgtagg tgacgccgta
    10261 gcgttcccac tcgtagtgca cggttgccga gtcgcaccat ccgtcgaggc cggcggcgag
    10321 ggtgaagtcg gtcatcgtct cgccgtacgc gatcgtcagc tcgcccggct cgacgtggtc
    10381 gattccgcgg agagaccagg tgcgggtctc gtcgcacacg aggcgcagac cgcacgcttg
    10441 cagcaccggt gcgaggaagc tcatcccgcc ctgcccggcc ttctgcacga gggattccgg
    10501 gtccagcggt gcgatcgggt agcgggtcga ggtggagtca tcgggtgccc cgtcccagct
    10561 gaccgtgtac ccgcctggcg gggtgtcgcc accgttgaac gcgggcacgt agtcgtccca
    10621 ctggacgacg ctcacgttcg tgacgaacac ccgctgatcc gcgcccgctg cgatcggccc
    10681 gaggaacgcg cgaacggcgc agtagacggc gccgatgggc gcctgcacca cggctgcgtg
    10741 tgtggtccag cgggtgtccg gggcggtcgg ctgaccgccc gggatgacct cgtaaatcag
    10801 ggtgaacgca ccgtcgaaga actggacgcg tatcttgcgg tcccggttcg gtgcgacaca
    10861 ggacgcgtcg aaggacgcgc tcacccagtc gccggcgctc accggcagag cggactcatg
    10921 cccggcgacc ggatcccacc cggcgacgat ggcgctgcca ccggcagtcc cacaggtgaa
    10981 ctgcagcgcc cgccgcgcgg tcgaccccgc cgcggtatgc gtggcggaga cgaggcccga
    11041 tgtgccagag cccgcggccg cgcccttggt ggtcgtctgg aaccgcggat tgacgacacg
    11101 gttcgtgagc gaccagtacg gggtgatatc ggccaccggg ccgggatcgg tgagggcgaa
    11161 cccagaccgg gccagcacca tctcagtcaa cacctcggcg acgtacccgt tggtgcgcag
    11221 atacgcccac gggcgcacat cagcatccgg ggtctcgtcc tccacgatcg cctcgtcgga
    11281 cgcgagctgc acctcgagca gcccatccgc gccgatcccg acgtgccgga tgccgagatc
    11341 gaactcccgg tgcaggccca tcacctgctc accacacgac agcaccaccc ggtgagccgc
    11401 cgtccgtggg tccatcgcgg cgtacagacc ggcccgcgcc ggcgtcctaa tggtcgcctg
    11461 cacgtgcggc cacgacgaca cggccaaccc gacctcaccg ccttcaacac caagctccac
    11521 gccgggcagt gcgtccgact gcgcatgcgc gtaatgctcc gtcaccgtca cggggagacc
    11581 tcctgaaacg ggactgagag aacccatgcg ctgcgagtct cgtcgtcgat ctccactcga
    11641 agctgcccgc ctgcgacgac gaaactcatc gcgaccgcgc cgacgtccgg gtcgctgagg
    11701 gcgagcacct gaggaacggc gagcacggtc tcggctgtca ccgctcccgc tgccgacgag
    11761 aagacgagac gcagctctcc ggagcgccgg ccagccggac gcagcgacac gtccggatcg
    11821 ggggagccca gcacaggatg aatgagggtg ccggcgtctc gcgcggcgtc gaatccgtcc
    11881 atgatgtcgg ggacgatgat tcccgaactg tgagtgatcg tcgtgaccat cagtcccact
    11941 cccctgcagg ccctacgatc ttcgatccga tcttgatctg gcggcctttg ttgatgaacc
    12001 gtgtaagagc gcggtcggca tccgaggtgt ccgcggcgac tttgataact gctttccggc
    12061 cgctcacatg gtcgatcacg ccgtccgcat ctccgcggaa cttggcgagg ttctcggatg
    12121 cctgcccggt cttcgcgtcg atgacgacgt ctcgaccctc gggcaagtgg atgagcctgt
    12181 tgccgaactc gtcgacttcc tcggtagcgt cctgcgcttt gctgatcgcg tcaagcaggt
    12241 agtcggaggt gatctggcga gctgtgacgg ccttgcgggc gttctcgtcg gtgacctggc
    12301 cgatgtcgta ccagcggccg tacagcgccg tcagatcctt gtcccaccgc tcggcgaggt
    12361 tctcgttcgc gagcgccttc tgctgcacgg cggcgacggc ggtctgcacc tcggcgagag
    12421 ccgcggtgtc gccggtctgc gcggcgagga tcttggacag gtcgaggccc agagtgatcg
    12481 catccttctg cgcggtcttc agatcctctg cacgctcggg atcgaagagg atatcgagag
    12541 ctgcgctgat cttggtctgg tcgtcgatga acgtgcgacc gtcctctgct gctccccggt
    12601 acgcatcgct gatgcgctca cggaacttgg cggcttcctc ctgctgcttc tccagctctg
    12661 cggtgacgag gccgagaccg accgcgccgg ccgcgaggac cgcggcaccg gcgatgcctg
    12721 cggggccggt cccggagagg gtggcggcga gcccgccgag ggtgtcctgt cctacctggc
    12781 cgaggtcggc gaggttgcct cgcacggacg agaccgcttc gccgaggttc tggccgatct
    12841 cctgggtgac ttcctgcgcg ccctccttca cgcgcgcgaa tccgtcgccg cctgcgcgca
    12901 caccgtctcc gatgtcgtcg ccggctttct tcccggcgcg gccggccttg ttgagctcgt
    12961 cgcgcagctc gcggatctcg tccttgctgt cctcggtgcg gcgctgcgca tcgcgcatac
    13021 tgcgttcgag gtcattgccg gcgtccttgg actcgcggcc cgtgcgctcc agcagatcgg
    13081 tgacgtcttc gagggggtcg atgatgccgc gctggatcgc ggacatggcc gagcgggtgt
    13141 cggcggctat cgagaggtcg atgcccttag ccattggact tcccttcgag ggcttcgtgt
    13201 attgcgcgga cgaatgtctg cacgtacatg gcgagtacgc gcgggatgat cgatgcggcg
    13261 gacgggtaga cgacgtaccc gcggcggttg ggtttcttga atgggcggtt cgggtcgcgg
    13321 taccgggtga acggggtgcc cttccggctg tgtgcttcct ctttcgcgcc ggggcggggg
    13381 ttggcgccga actccaccgg cccgtaggac tcggatggtt tcaggccacc ggagaggctg
    13441 cgagacaggc tggcggacgt gagacgcacg ttctggtccg agacacgcgc ccgcccggtt
    13501 gagaccagca cgcgcgattc cagcgaggtg ctggcatgct tcgcgagttc ggcctgccac
    13561 gcggtgagca ccatgtcttt gagggactgg cgcaggtggc gtttcgtgtc ccggtcgagg
    13621 gtgcggagcc cgcgcagcac cgtgagcagc tcacggctgc tcagggcgct gacgcggagc
    13681 atccgacatc accccggtta cgcgccggcg agcgtgcggg tcggcttgcc cttggcgggc
    13741 atcgtcacgg acgcggtgcc gaactggtcg acgtccgcgc cgatggtcgc ggcctgcacg
    13801 atgatcgttc cggcgaaccc ggtgccgccc gcaatcggct ccagcgtgaa cggcacggtc
    13861 tcgccctcgt gatcgaacag gtagttcgac aggctggtcg atgtctcatg gtcctgaccg
    13921 agattcacgg tcagcgccca ggtcgcgagg cctgcagcgg tgaacacgct cccgggcttg
    13981 agccccttcc acgtcgcgct ggacgaggtg ggaacgagct gcgcaccgga gatctccgcg
    14041 gcgaagtcgg tcggggccgt gccgagctgc agcacgcttt cggtcatgta gagggggttg
    14101 aaggccatgg tcacgcctct ttcttgatgg tggtgagagc ttcgagctgg atgtcgtagc
    14161 agaggtactt ggactggaac tgcgccttct cagcgctgcg gaacgccacc caccggatcg
    14221 gctggatagc ggcgatcagc tcgtcaagca gcccgtcaag gtcgatgtcg gcctgcacct
    14281 ggtcggcgtg ctcgctgacc acggtcacga cgaacgaggg catgagcacc tgctgtgcgc
    14341 ccgggtgccg gctgacggtc gagagcttga gcatcagcgt cggtttcgcg ggccggtcga
    14401 ggcgggtctg cgcgggcacc caccggaact tacccggctc gatgttcgcg agcaggtcgt
    14461 tctccagctg cgcacggagg tcaccgaatg cggtcacgcg atcacctcgg ggaactcgtc
    14521 gtggccggtg gcgaggctgt tgccgccgcg ctgcgggcgc atcatgcggc gggcctcgcg
    14581 ggcgagccac ggcggattgt aatcgaaacc atcagcgccg agcaggccgt cttcaccggc
    14641 gagagcgtcg ttccacaggc cgcgcacgag ggtgagctga ccgagccgga tggtcgcgtc
    14701 ccggggacgt tcggggtcgc cctccgcggg cacccatgcc tcgcaggcgt cctgcgccgc
    14761 gtcgagcatc ggctgcagca tctcggaggg aacgccctcg gagtccggcc acagggccgg
    14821 caggtcgtcg ggggtgatcc agtcgacggc catggctcgt cctctcaggt ctcagggatg
    14881 gtgggcggtg ccgcccgcgc ggtatggggg acgcgggcgg caccgggtca ggcgcccgcg
    14941 ggggtcaggg tgacgttctg gatgcccttg gcgtagtagt cgcggaactg cacgtagccg
    15001 aacagtgcct cgtccacacc gcccttcacc atgtcgagcg cgttcacgcg gatcgggctg
    15061 ccgggcatct cgtgcacggc ggctgcctcg cgggcgccga ccgtgaccgt gccggccggg
    15121 atgcccgtgt gcgggacgat cttgaatccg gagagatcgc cgtcctccag accgagcgcg
    15181 aggttcaggt actccagcac gtcgctcttc ttggtgaaca gcagcgggcg gtagtcggcc
    15241 gggttgacga gtgcgtacga cggacgcgcg tcggcggcga tcagcgccag cgcaccatcg
    15301 acgatgccga cgagcgctgc gggcacgtct gcgggaacgg ctgcggcgat gttcatcgcg
    15361 gtgcccgctg cgagcagctt ggtcagcacc cacgcgtccg acttcttggc gtacgaaccg
    15421 gcggcggcgg tcaggaacga gttgatgacc tcggtctcgc cgaagtcgat gaactcgcgg
    15481 gcgatgtccc atccaccggc gaaccgctgg aatccccacg tcgcgggctc ggtcgccggc
    15541 gtgttgctcg ggaccgcggc cttgttgccg gcccactccg cgacctcggg ctcggtcgtc
    15601 caccgccacc cggtcgcgct cagcgacgtg aggtcgccgt gctgcagcag cgggatgacg
    15661 cggcgaacga acgggatgcc ggaccagatc tctccgatga actcgggcgg gatcgcgccg
    15721 gtgccgaccg cgttcgcgcc gtcgatcttg acgtcgttca gtgccgcgag catggtctcg
    15781 cggttctcgg gctggcggat cgcgttcgcg atcatcgccg agaacgtcgc ggacgtgatc
    15841 gggtcggcct tgtccttctc tgccttcgac gcgagcagcg tggacggcac ctgtgcgttg
    15901 gccatgtcgg cctctccttc atccggctcg gccgggtctg tggtttcggt gatggtggtc
    15961 gttgtgatcg tggtgacgcc ggcatccgtg gtggtctgct cggtggtcga gtcgtacacg
    16021 cgccggtact cccggccgtt ctcgtcggtg tagacggtct cggtgtggtc ctcgccgatc
    16081 acgccgtcag cgacggcgaa cagtgccgcg gatgcgaacg ctccttcgtc gaccgcggcc
    16141 gctccggtga gcttcgagcg gacggcgttc gcaccggccc gcacgagctc ggcgacctcg
    16201 gcggagagct tgcgcagcgg cgaggcgagg aacgaatcgc cctcgtcggt ctccgcgatc
    16261 tcgaactcgg cgacgacgcc ggccggggtc ttctccagcg cgacggcccg gccgatgggg
    16321 tcgaaccggt cgtgcttccg gttcagggtg accactgacg ggtcacgagg cagggtgatg
    16381 ctgtcggcgc tgaacatgac cggctcgttg ccggacagat tgggtcggga cagttcaccg
    16441 aacggcagca gcaggccgcg cacacggcgg gtgccgtcga tgcgctggaa cagtccggtc
    16501 tcggtcatgg gtatcagtcc tccgtggggg taccggtctg cgccggtgtg gtcgtggcga
    16561 agtcgaagcg gatacgggtg ccgcggggca ccacgtcatc catcgacagg cggtgctcca
    16621 gcggggccgt ccagaacgag aggcggtcct gcagctcggc gcgctcggtc tcctgtgtct
    16681 cgtaggtgag cgagctctgc gccttcgcgc cgtcaagagc ggacgcgttg aggttcagca
    16741 ggttcgcgat gtcgatgcgc accgcgttgc gtgccttgtc gagaacatcg atcgtgccgc
    16801 ccgactccag ccgcaggttg atcttctgcg ggacgaagaa caccgccccg tccgggtcgc
    16861 gcagcgcggt tgctacggcc ttcgtgtagt tgttcgcttc cttcttgctg accgtgccgt
    16921 cctcacgttc ttcgaggatc acggtcgggg cggggttgcg gacgcgggac gaccacgcgc
    16981 gctccagcgc cttcgccgcg cgcacggtct cggatgccac gttcagcagt ccctcgaacg
    17041 ggccgggaag gtagatcacg gactcggccg gcacgggctg ctcctcgacg aggatctcgc
    17101 gggtgtcggc gtcgacgcgc caccggtcgt acggggcgtg cacggcgtcg aggatcggcc
    17161 cgtacttcga cccgttcggg atcgcgccac gctccagcac ccacagcgag tcgccgtaga
    17221 agatccagtc atccagggtc gaggcgatcc gctgccacgg cgtgacggcc gtgttcgtgc
    17281 ggtacaacca cgactgcgac tgggtgacgt cctccgcacc gcgcaacgca cgcagaggcc
    17341 ggtctgcaag ctgcacgatg cggtgacgag acgcggcgac cgccgggacg ctcatcgctt
    17401 ccttgcgggt caccgggacg acgttcaggc cgtagatgtc ggcccacacg aacgagttga
    17461 gctggccgtc cgaccatggc gacacgatcc catccgcatg cgcggcatcg acgccgggca
    17521 tcttgagcag agtgccggac tcaatgtcct ggatcgcgga gagccgccgg gagaacttca
    17581 cgctctccac gatgcggacg gggtgttacc taaccggcgc gagacggcgt gtcgggtgct
    17641 cttcgtcgct ctgctctccc gtccgggcgg tctgcaccct tgaccaactc gccgccatag
    17701 tcctcccagc aggccggaca ccacttccga cgacgagtac gcacaccgcg atacccgcag
    17761 gcgctgcacg tcgcgttcgg cttccgtcgt aggtgccgcc ccgaccacag acgcaggcgc
    17821 gtccagggcg tcggttccca gtagaacggg tcgccgatgc ggaatccgag attgagcagc
    17881 agtcgcttca tcgctcatcc tcactccgga tcgggcgatc cggcgccttc cgccagcagg
    17941 tcgcgcagca gtgtgcctcg gtcaagtcgt gctgactcga cagcggctcc atgagcgaca
    18001 ccgcgcctgc gaagtagcgt cgacacttcg acggctcgcc ttcggcggcg aagtagtgca
    18061 gcttcgcggc ttcacgagtc gggtacgccc atccgggcag ggttgccgtg ctcatcgctt
    18121 actcccgtcc gggcgatctg cacccacgag cgcgagcatg ccgtcagccg cggccgcgac
    18181 cgctcccgat ccggggaaca gatcatccag cgtgtcgtca ccaggtgcgt acccgagcgc
    18241 atcgagcacc caccgcgtcc acgcggcagg cttcgcccca gcgaatcccg gtcggccagc
    18301 tggcgcatcg agcacgtcgt tcgtgaccgg ccatgcaccg cggccgcgcc ggtctgcggg
    18361 ctgacggatg atgaccggct cccagacgcg gcgcacacgg ttcccggtcg gcggcgcgtt
    18421 gcgacgatgc cacaccatca cgtgcacgtc atccgggcac tcgaccaggt agcgaccgag
    18481 cgagtccggc gcgagggcaa tcgcccatcc gtcgtattca tccacgaggc gctgcaccag
    18541 atgagcgtga gcgggccacg agtcccagat gcctgcctcg ggatggtgat cagcgacacc
    18601 gcgaccggag tagtcgccgc gcccattccc gtaccagcgt tcgccccgac caaagtacgg
    18661 cgggtctgca atcgcgagct tagccatcgt tccgactccc atccgggcgg tctgtgcccg
    18721 tcatgaccgc ccacttcggc gggtcagtct cggtgtatcc ggcctcggtc agcaggtggt
    18781 ggatcgcctc ttcggtcacg ctgacgatgc ccattccgtc gacatcgacg gatgaccact
    18841 tgtcgcggcc cggcgacttg taccagcgct tgtgcggctc gcgcggcgat ccctcgatac
    18901 cgtcgtacct cagcctcagc gtcatgactc gctcccgttc tcgcggttgg tggtcttgtg
    18961 ttcgtgctgt cggcgcgggc cgctggcggt ggtggcgtcg atgtcgtgga cgttcatcag
    19021 gtgtcgttct ccttgccggt aggcggcgtc gagggtgaac tcgaacgcgt gccagtaggt
    19081 gcagtcggtg caccagctgt tgacgtagcg gacctgtccg cctttcacgt cgcgggtgat
    19141 ctcacggcgg atggtggcgg tcatgcggcc acgatcccgg tgtcgtcatt gcgggacttc
    19201 gcgtcgaagt gctgatccca gttccgcagc gcgcgcacgg ccgcgtcgag gcacgtgatg
    19261 tcgtcgccgc gttccatagg ggtgaacaac cacacgccgt cgtcgttgcg ggtggtgcgc
    19321 ttcgcggcgc gctccacagc gacgtcgagt ccggtctgtc cggtgaagtg gatcaggctg
    19381 ccgcgttcca ggtctcgcag gaactggatg cagccggctg cggtctcccg gtaggtctgc
    19441 atccgcatgc ggggcttggg gcgcagacgt gcggcctcgg tcgcggtggc cttgccctcg
    19501 gcgatgtcgt cgaacgcgat cgtgctgccg gggtacctct tcgagagctc ctgcatccgc
    19561 ttggggagcc acagcgttcc ggtgcggtgg tcgacaactt cgagtaccgc gcgtcgcttc
    19621 ccgtcgcggt aggcggcgac gatcgcggcg acaccaccac cgggcttgac agcgacaccg
    19681 aacgcgacct tgcgaggaag gggcgggcgc ttcccgcgct cggtcttggt ccaccagtcg
    19741 cccgggatcg cgcgttcgcc gaacgtctcg ggccacagtg atccgtactc gcgcgcccac
    19801 ttcggcttgt ccatcttccg ccagttgctg cgctgcttct cgatggtcgt gagcgtcccg
    19861 acgccggggt gatgcagctg gatcagggcg agcgcgtctt cctcacgctc gatggactcc
    19921 cacggcgtgt caggcgggaa gcagtagtca gctccggcga tgagcccgcc cgcgcgcaga
    19981 cgctcgatgc gctcccatag cgggccgctt cggatctcgc ccgcggtgcc tgacacgacg
    20041 agcgcggagt tctcgcgggt gtcctgcagc gggatgatgc cggccagcag gtcggcgccg
    20101 tcctcgggat cgagttcctg cgcctcgtcg atccacgaga tgtcaccggc ctcgccgcgg
    20161 taggcgtccg cgtcgggctt gagcacgagg aacgtcgaat cgttcgtgta gtagatcccc
    20221 ttgccgactt cgcccatcat gatgcggaac cggcgaccga gaggctcggt gtcggcggct
    20281 ggcgcgagct cttcgccgaa gagagcaatc tgccgctgca ctgctttcgg ggtggtacgg
    20341 atgccacgct tccacggcgg gatgccggct gccgcttccg ggtcggggtc ggtgcggtcg
    20401 aggcgggact tccactcacg gagccgggcg ccggacttca gaccggactg cgccgagaac
    20461 gtgacctggt agttcgcgcg cgacaggcac cgaccgtggc accacatgaa gatcgtcgtc
    20521 gtcttcgacg cccggcgcat ggcctcgacc acggcctcgt cgatgtcgtc gatgttgccg
    20581 gccgcgttga gcgcatcgac gatccacagt tgctgcggct ggagcgggaa ccgcttcggc
    20641 gactcagcgg ccatggccgc gatggtctcc ttgtcgtcga ggtcgaccag ctcgtaaccc
    20701 atcgagcgag cacccacgag gaactcacgg cgcagctcgt cgtcttcgag cagcggcccg
    20761 aggaactcgg ggcggatgcc gcggttccgg gtcgcgtccc acagatagag agagaaactt
    20821 gtgctgcccg tatacggagg tacagacgct gtctcaaaaa ctggtgcgct caccatgatc
    20881 ggatgccctc tcgtgcgtcg gtgcgtcggc gtcgtccgct gttggtcgcg gctgcgccga
    20941 gcttgccgcc cgcgcgcagg ttgcagagcg cgtgcgctgc gccggtgttc gccctcgtgg
    21001 tgcggccgcc ctgcgatgct ggggtgaggt gcgcgacgtg ccacttctga tcgcggtaga
    21061 cgggcttgcc gcactcggtg cacggcaggg gcagtcgggc ctcgatctcg gggcgcagct
    21121 tgtgcgtggc ggccgtgtgg tggctggcgc ggtggtggcg gctcacgatg cgtcaccgcc
    21181 tcgagtgtcg atccagcatc cgagcttgta ggcgatccac gcgcccacgg cgagcaggat
    21241 ccacatgccg ttgatgatca tcggttcagc tcctgcggtt cggggggggt ctgcacgcaa
    21301 cggaagaact cccacgcggc ccgctcgttg gcgaggatgc ggcggccggc gtcgtagaac
    21361 gcttcggccc tcgtgtactc gtaggcggtg ccgagcacgg cgcgagcgat ctgggcgagt
    21421 aggcgggcct gccgctgcat gtccttcggg atgccggtct ctggcttcca ccgctgggcc
    21481 agctcggcga actgctcggc ggtctcgtac agcgcgtggc tcaacgcgat gcggtcaagg
    21541 tgggtgacgg tcccgtcgtg gatcgtgctc atgctgcggc tccttccacg tctgctgcgc
    21601 ggcatccgcg cgagcagtgg cggtcatcga tcaagtcgtg cccgcacgcg ccacgcgcac
    21661 ggttaccaac gtgagttgat ttatgagaag tagtagaggg ttggttatag gacggttcgg
    21721 gcggcgctgt cgccgcccct cccctggcgc tgtcgccacc gggcggcgct gtcgccgccc
    21781 gtgtggataa ctcctcgatc tcggtctgac cggtgcgcga aacgcgcgta cggtggtgcc
    21841 gtgatccgtc gcaatcgggc gggcatcgca acacgaagtc gtagaggttg ggacggcggg
    21901 catcagccgt gctcgagtcg ccgccacgct ggatcttggc gcggatctca tgcagctctt
    21961 cgagcttccg gacggcgcgc tgaacgttgc gccggtcgac gccggcatag cgggagagcg
    22021 tggcaacgga cggccacgct cccccgtctc catcgtggtt agcgatgccg atcaggacga
    22081 gcttggccgc acccgtcgcg cgcgagtgat gcaacgcgat cgagatcgac tcaacgctca
    22141 tgacgtcact tagcgtcgtc ggggtgcaca gcaccgatcg ccgtgcgcca cgggccggtc
    22201 agcaggtcat agtgatcctg cgtaaaaccg tgctcgccga tcaggtcacg caccaccagc
    22261 cctgcggcgg cccctgcggc gtccctcgcg gcggcccctg cggcggcccc
//
LOCUS       DATMSX010000316         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_90303,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000316 DATMSX010000000
VERSION     DATMSX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90303"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VN041_09730"
     CDS             complement(<1..1536)
                     /locus_tag="VN041_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWU29355.1"
                     /translation="MFQKILVANRGEIAIRAFRAAYEVGARTVAVFPYEDRGSLHRLK
                     ADEAYEIGERGHPVQAYLDVDGIIAVALDAGADAIYPGYGFLSENPELAEKAAAAGIT
                     FIGPPAQVLEMAGNKVEAKRHAVEAGVPVLRSTEASDDVEALVAQADEIGFPLFAKAV
                     AGGGGRGMRRVETKGELAPALAEAMREAGAAFGDSRMFLEQAVLRPRHIEVQILADGQ
                     GNTVHLFERDCSVQRRHQKVIEIAPAPNLDERTRSDLHRHAIAFARSIGYQNAGTVEF
                     LLETAGPRTGEVVFIEMNPRIQVEHTVTEEVTDVDLVRAQMQIANGATLADLDLEQDD
                     IQLRGAALQCRITTEDPTQGFRPDTGKITTYRSPGGAGIRLDGGTTAAGSQISPYFDS
                     MLSKLTCRGRSFNSAVSRARRALAEFRIRGVSTNIPFLQAVLDDPSFAAGDLSTSFID
                     ERPELLRGRVSKDRGSKILRWLVDVTVNKPNGENPLALPPAHKLPALDLGVEPPAGSR
                     QQLQ"
     gene            1697..>2470
                     /locus_tag="VN041_09735"
     CDS             1697..>2470
                     /locus_tag="VN041_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWU29356.1"
                     /translation="MHVLSVSSLKGGVGKTTVTLGLASAAFARGIRTLVVDLDPQSDV
                     STGMDIQIAGRLNIADVLENPKEKVVRQAITSSGWAKVHPGTIDLLIGSPSAINFDGP
                     HPSVRDVWKLEEALATVESEYDLVLIDCAPSLNALTRTAWAASDRVMVVTEPGLFSVA
                     AADRALRAIEEIRRGLSPRLQPLGIVVNRVRPQSIEHQFRIKELRDMFGPLVLAPQLP
                     ERTSLQQAQGAAKPLHIWPGDSAQELAADFDQLLDRIIRA"
BASE COUNT          363 a          881 c          836 g          390 t
ORIGIN      
        1 ctgcagctgc tggcgcgagc ccgcgggcgg ctcgacgccg aggtcgagtg cggggagctt
       61 gtgcgcgggc ggcagagcca gcgggttctc gccgttgggc ttgttcaccg tgacgtcgac
      121 gagccagcgc aggatcttcg atccgcggtc cttcgacacc cgcccgcgca gcagctcggg
      181 acgctcgtcg atgaacgagg tggacaggtc gcccgccgcg aaggaggggt cgtccagcac
      241 ggcctgcagg aaggggatgt tcgtcgagac gccgcggatg cggaactcgg ccagtgcgcg
      301 tcgtgcgcgg gagaccgcgc tgttgaacga gcggccacga caggtcagct tggagagcat
      361 cgagtcgaag taggggctga tctgggagcc cgccgccgtg gtcccgccat cgaggcggat
      421 gccggcgccg cccggcgagc ggtaggtggt gatcttcccc gtgtccggcc ggaagccctg
      481 tgtcgggtcc tcagtggtga tgcggcactg cagggcagcg ccacgcagtt ggatgtcgtc
      541 ctgctcgaga tcgaggtcgg cgagggtcgc gccgttcgcg atctgcatct gcgctcggac
      601 gaggtcgacg tcggtgacct cctcggtcac cgtgtgctcg acctggatgc gcgggttcat
      661 ctcgatgaag acgacctccc ccgtgcgggg tccggccgtc tcgagcagga actcgaccgt
      721 tcccgcgttc tggtagccga tcgaccgcgc gaacgcgatg gcgtgacgat gcaggtcgct
      781 gcgggtcctc tcgtcgaggt tcggcgcggg ggcgatctcg atcaccttct ggtgacggcg
      841 ctgcaccgag cagtcccgct cgaacaggtg cacggtgttc ccctgaccgt cggccaggat
      901 ctgcacctcg atgtgccggg ggcgcagcac cgcctgctcc aggaacatcc gcgaatcgcc
      961 gaacgccgcc cccgcctcgc gcatggcctc ggccagcgcc ggcgcgagct cgcccttggt
     1021 ctccacgcgg cgcattccgc ggccgccgcc tccggcgacg gccttcgcga acagcgggaa
     1081 gccgatctcg tcagcttgag cgaccagcgc ctcgacgtca tcggatgcct cggtcgagcg
     1141 cagcacgggg acgccggctt ccactgcatg ccgcttggcc tccaccttgt tgccggccat
     1201 ctcgagcacc tgcgcgggtg gaccgatgaa ggtgatgccc gccgcggcgg ccttctcggc
     1261 cagctcgggg ttctcggaga ggaatccgta gcccgggtag atcgcatcgg ctccggcgtc
     1321 gagcgccacc gcgatgatcc cgtccacgtc gaggtaggcc tgcacggggt gcccccgctc
     1381 gccgatctcg tacgcctcgt ccgccttcag ccggtgcagc gagccccggt cctcataggg
     1441 gaacacggcg acggtccgag cccccacctc gtacgccgcg cggaatgcgc ggatcgcgat
     1501 ctcaccacgg ttggccacca ggatcttctg gaacatgcac acctcttctg aggtcgtcgc
     1561 gcgccggatg ccgcgcgcgg agcggaggct gagtgtgtcc ccacactaac gtgtgccgca
     1621 cgtcgactcc gccatgtgaa gccgacctgc ggggcacgga ggagactcac ggcaacggca
     1681 cggtaacgtg taggtcgtgc acgtactcag cgtcagctcc ctcaagggag gcgtcggcaa
     1741 gacgaccgtg accctcggtc tcgcttccgc cgccttcgcc cgcggcatcc gaaccctcgt
     1801 ggtcgacctc gacccgcagt cggatgtctc cacgggcatg gacatccaga tcgccggccg
     1861 actcaacatc gccgacgtct tggagaaccc caaggagaag gtggtccgtc aggcgatcac
     1921 cagcagcggg tgggcgaaag tgcacccggg cacgatcgat ctgctgatcg gcagcccgtc
     1981 cgcgatcaac ttcgacggcc cgcaccccag cgtgcgcgac gtctggaagc tcgaggaggc
     2041 gctggccacc gtcgagagcg agtacgacct cgtcctgatc gactgcgctc cgtcgctgaa
     2101 cgccctcacc cgcaccgcgt gggctgccag cgaccgcgtc atggtcgtca ccgagcccgg
     2161 actgttctcg gtcgccgctg ccgaccgcgc actgcgcgcc atcgaggaga tccgccgggg
     2221 cctctcccct cgtctgcagc ccctcggcat cgtcgtcaac cgcgttcgtc cgcagtccat
     2281 cgagcaccag ttccgcatca aggagctgcg cgacatgttc ggcccgctcg tgctggcgcc
     2341 gcagctgccc gagcgcacct ctctgcagca ggcgcagggc gcggccaagc cgctgcacat
     2401 ctggcccggc gactccgccc aggagctcgc ggccgacttc gaccagctgc tggaccgcat
     2461 catccgcgcc
//
LOCUS       DATMSX010000317         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_175349,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000317 DATMSX010000000
VERSION     DATMSX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175349"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VN041_09740"
     CDS             <1..620
                     /locus_tag="VN041_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29357.1"
                     /translation="ASVQRAQLQVLGRDRARLEAAGDASSGERVIELSARHAEILLML
                     AVHRQGLSADRLAELVYGEGASPDTLRPEMVRLRKVLERISPELVPQSRPYRLPIALE
                     TDAQNVLSLLDRGAHRVALSAYRGPVLPDSDAPGVVELREGLRGTLRETMLTEASVDV
                     LLSYADIPEGADDAEVLRLCLEMLPARSPRRAGLVSRIEKLEASA"
     gene            727..2274
                     /locus_tag="VN041_09745"
     CDS             727..2274
                     /locus_tag="VN041_09745"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU29358.1"
                     /translation="MTIVEESVSTAYAAPGRPGAIANYRPRYGHYIGGEFVDPVKGQY
                     FENISPVNGKPFTEIARGTAEDIDRAVEVAWKAFESWKRTTPAERSVILNRIADRMEQ
                     HLEEIAVAETWENGKPVRETLAADIPLAIDHFRYFAGVLRAQEGGISQLDENTVAYHF
                     HEPLGVVGQIIPWNFPILMATWKLAPALAAGNCVVIKPAEQTPASILFLFEIIGDLLP
                     AGVVNIVNGFGIEAGAPLAQHKRIRKVAFTGETTTGRLIMQYASQNLIPVTLELGGKS
                     PNVFFEDVARADDAFYEKSLEGFTMFALNQGEVCTCPSRALIQRSIYDGFLSDGLARV
                     GKVIQGNPLDPATMIGAQASNDQLEKILSYIDIGKQGGAKLLTGGECVDLGGDLSEGY
                     YVAPTVFEGTNDMRIFQEEIFGPVLSVTSFSDFDDAISIANDTLYGLGAGVWSRSGDT
                     AYRAGRAIEAGRVWTNTYHQYPAHAAFGGYKQSGVGRENHKMMLDHYQQTKNLLVSYA
                     EGPMGFF"
     gene            2341..2742
                     /locus_tag="VN041_09750"
     CDS             2341..2742
                     /locus_tag="VN041_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HWU29359.1"
                     /translation="MVSIGRYERVDVTDAAASLIRDLTSQHGPLMFHQSGGCCDGSSP
                     MCYPVGMFITGPSDVKLGDLDVGLDAPVEMYMSESQFEYWKYTHLTVDAVPGRGAGFS
                     VEGPTGMRFLTRSRMLNDAELEYFGLAPAQS"
     gene            complement(2753..>2935)
                     /locus_tag="VN041_09755"
     CDS             complement(2753..>2935)
                     /locus_tag="VN041_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29360.1"
                     /translation="PGPLPFAPPAGMLAPPPGVAVQGRRLKRGVSWVTIVAILLMLGF
                     FIVPQFLGVLGFALFR"
BASE COUNT          490 a          995 c         1030 g          420 t
ORIGIN      
        1 gtgccagcgt gcagcgggcc cagctgcagg tgctcggtcg ggaccgcgcc cggctcgagg
       61 ccgccgggga cgcgtcttcg ggggagcggg tcatcgagct cagcgcccgg catgccgaga
      121 tcctgctgat gctcgcggtg cacaggcagg gtctctcggc ggaccggctg gccgaactgg
      181 tctacggcga gggtgcgtcg ccggacacgc tgcggccgga gatggtgcgc ctgcgcaagg
      241 tgctggagcg gatctctccc gagctcgtac cgcagtcccg cccctaccgg ctgccgatcg
      301 cgctggagac cgacgcgcag aacgtgctct cgctgctgga ccgcggtgcg caccgggtgg
      361 cgctctcggc ctaccggggc ccggtgctgc ccgactccga cgccccgggc gtcgtggaac
      421 tgcgggaggg gctgcgcggc acgctgcgcg agaccatgct caccgaggcg agtgtggacg
      481 tgctgctcag ctacgccgac atccccgagg gcgccgacga cgccgaggtg ctgcgcctgt
      541 gcctggagat gctgccggcg cgctcgccgc ggcgagcggg tctggtcagc cggatcgaga
      601 agctcgaggc gtccgcctag tcgcaacccg atgcaacgtg acgcacggga ctgcaacctc
      661 ggacgaccta ctttcgacgc atcagatcgt gcggcatcgg agccgccgga tgtcgaagga
      721 gacgccatga ccatcgtcga ggagagcgtg tcgaccgcct atgccgcccc cgggcgcccg
      781 ggggccatcg cgaactaccg cccgcgttac gggcactaca tcggcggcga gttcgtcgat
      841 cccgtgaagg ggcagtactt cgagaacatc agccccgtca acggcaagcc gttcaccgag
      901 atcgcccgcg gcacggccga ggacatcgat cgtgcggtcg aggtcgcctg gaaggcgttc
      961 gagtcgtgga agcgcaccac gcccgccgag cgctcggtga ttctgaaccg gatcgccgac
     1021 cggatggagc agcacctcga ggagatcgcg gtcgccgaga cgtgggagaa cggcaagccc
     1081 gtccgcgaga cgctggccgc cgacatcccg ctggcgatcg accacttccg gtatttcgcc
     1141 ggggtgctgc gggcacagga gggcggcatc agccagctcg acgagaacac ggtcgcgtac
     1201 cacttccacg agccgctggg cgtggtcggc cagatcatcc cgtggaactt cccgatcctg
     1261 atggccacgt ggaagctggc accggcgctc gccgcgggca actgcgtggt gatcaagccg
     1321 gccgagcaga ccccggcatc catcctgttc ctgttcgaga tcatcggcga cctgctgccg
     1381 gccggcgtcg tgaacatcgt caacgggttc ggcatcgagg cgggcgcccc gctcgctcag
     1441 cacaagcgca tccgcaaggt cgccttcacg ggtgagacca ccacgggccg cctgatcatg
     1501 cagtacgcct cgcagaacct gatcccggtc acgctggagc tcggaggcaa gagcccgaac
     1561 gtgttcttcg aggacgtcgc ccgtgccgac gacgccttct acgagaagtc gctcgagggg
     1621 ttcacgatgt tcgcgctcaa ccagggcgag gtgtgcacct gcccgtcgcg tgcgctgatc
     1681 cagcggtcga tctacgacgg cttcctctcg gacgggctcg cgcgggtcgg caaggtcatc
     1741 cagggcaacc cgctggaccc cgcgaccatg atcggcgccc aggcgtcgaa cgaccagctc
     1801 gagaagatcc tcagctacat cgacatcggc aagcagggcg gtgcgaagct gctgaccggc
     1861 ggcgagtgcg tcgatctggg cggcgacctc agcgagggct actacgtcgc accgaccgtg
     1921 ttcgagggca ccaacgacat gcgcatcttc caggaggaga tcttcggccc ggtgctctcg
     1981 gtgacgagct tcagcgactt cgacgacgcg atctcgatcg cgaacgacac gctgtacggc
     2041 ctgggagccg gcgtgtggag ccgcagcggc gacaccgcct accgtgccgg ccgggcgatc
     2101 gaggccggcc gcgtctggac gaacacgtac caccagtacc cggcgcacgc cgcgttcggc
     2161 gggtacaagc agtccggtgt cggtcgcgag aaccacaaga tgatgctcga ccactaccag
     2221 cagaccaaga acctcctggt ctcgtacgcg gagggcccga tgggcttctt ctgatccgcc
     2281 gtccgggcgg ggccgggagg ccccgcccgg attccatgca cgcaggaggg aagagggccc
     2341 atggtcagca tcggcaggta cgagcgggtg gacgtcacgg atgccgcggc atccctcatc
     2401 cgcgatctca cgtcgcagca cggcccgctg atgttccatc agtcgggcgg atgctgcgac
     2461 gggtcgtcgc ccatgtgcta tccggtcggc atgttcatca cgggaccgag cgacgtgaag
     2521 ctcggcgacc tcgacgtcgg gctggacgcc cccgtcgaga tgtacatgtc ggagtcccag
     2581 ttcgagtact ggaagtacac gcacctgacc gtggacgcgg tgccaggccg cggcgccggg
     2641 ttcagcgtgg agggcccgac cgggatgcgg ttcctcaccc gatcgcggat gctgaacgac
     2701 gcggagctgg agtacttcgg gctcgcgccc gcgcagtcct gagcgtccgg cgtcagcgga
     2761 agagggcgaa gcccaggacg cccaggaact gcggcacgat gaagaagccc agcatgagca
     2821 ggatcgccac gatcgtcacc cacgagacgc cccgcttcag acggcggccc tgcacggcga
     2881 cgccgggcgg cggagccagc atcccggccg gcggcgcgaa aggcagcggt ccggg
//
LOCUS       DATMSX010000318        13085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156622,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000318 DATMSX010000000
VERSION     DATMSX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156622"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..7406
                     /locus_tag="VN041_09760"
     CDS             261..7406
                     /locus_tag="VN041_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29361.1"
                     /translation="MNKYVSRGLLLAGFAGGLWVAGTGVAAAAETPAGDEAPAVVNVQ
                     LPLTLEGQAVSATGTSTQDPPAEKPKPAPIVSVDLKAVTGVLKGEKAVAAVSTPVTVS
                     GNAVSVTGNAKSTASSVTQAPAQPKDAPSSNDPLGVLTGAQGLVDVSAPVTVAGNAVS
                     VFGDAESHGSAVTTTGAGGDGDGSALENEGIGVGTGWQAAGDVTAPLTAAGNAASVFG
                     DASSNDSRVDVRDADGTAGGAGTISNSGIGLNTGWQVPADVTTPLTAAGNAGSLFDDA
                     ASNGSQVAIEDRSGVANSGGRIENSGIGKDTGWQIPADVVAPVTFSGNAISGTGEAVT
                     SGSDVAVSDRAGVANGGGEVSNTGIGWLVGWQIPADVSAPLTASGNAFSATGDVASRD
                     SGVTVADSAGVANSGGEVGNAGYGVGSGWQIPADVIAPVTVSGNAGSVLGDAGTSGSD
                     VAIVDRSGVASGNGEVTNAGYGVGAGWQIPADVTAPLTFSGNAVSGSCDAVTTGSDVS
                     VLDNAGVADGGAASNAGYGVGAGWQIPADVTMPVTASGNAGALLDNATSTGSGVAIVD
                     NGGVADGGSEIGNAGFGLNSGWQIPADVTAPVTLAGNAVSAGNEAVTTGSGVGIVDDA
                     SVADGSGEVSNVGVGKDTGWQIVTDWTTPLTASGNAVSGAGDAVTTDSGVGIVDRGDA
                     VDGAGEASNTGIGWLVGWQVLGDVTAPATASGNSGSVLGDAGTTGSTVGVVDNAGVVN
                     GDGDVSNAGYGVGAGWQVLGDVTAPVSFSGNAVSGSGDSLTTDSGVAVVDDAGVIDGD
                     GDVSNTGIGLNSGWQVVTDWTTPGTASGNAISGVGDASTAGSDVAVVDRAGVIDGSGV
                     ISNDGIGKDSGWQVAGDVTVPLTASGNAGSAFGDADAVDSGVGVVDDTAVIGSAGQAT
                     DNGVGFGTGWQVVGDVVVPVTGSGNAASGFGDVVTDGSEVAVLTEGGSIVSGHADDSG
                     IGVASGWQVVGGVVAPITASGDAVTVAGDTGSHDSLVTVHGSNGDLGDGALGNEGVGV
                     GTAWQPVVEITTPVTVSGDAVAPVGAVSGDSTVVVEPGMSGSLNPGLPGVPSIPVPDL
                     GDAAGDALGNVHGLPSLPGGELVGGVIGDVIGAGEPGDPAVPGAGDLIDGVIGAVPGL
                     STAPGVPELGGSLGDAVDSIGTVIGGGLPEVPTEPSVPGEPEVPSLPVIGALPGVGEV
                     VGTLPGVGGVIGSLPGIPALPVLPGEPHGPVLDLGDLVGDITDCPCDAIGGVVDTVGD
                     LGGTIGDVAGSIGDIAGGLPGLPGGDPSVPGLPGLPGLPDLGGVVGDVVDTVGGLVPG
                     LPELPGLPGGDPTVPGLPGLPGLPDLGGIVGGVVDTIGGLIPGLGELPGLPGGDPTVP
                     GLPGLPGLPGLPDLGGVVGGVVDTIGGLVPGLPELPGLPGGDPTVPGLPGLPGLPDLG
                     GVVGGVVDTIGGLVPGLGELPGLPGGDPTVPGLPGLPDLGGVVGGVVDTIGGLVPGLG
                     ELPGLPGGDPTVPGLPGLPGLPDLGGVVGGVVDTIGGLVPGLPELPGLPGGDPTVPGL
                     PGLPGLPDLGGVVGGVVDTIGGLIPGLGELPGLPGGDPTVPGLPGLPGLPDLGGVVGG
                     VVDTIGGLVPGLGELPGLPGGDPTVPGLPGLPDLGGLIPGLPGLPDLGGVVGGVVDTV
                     GGLIPGLGELPGLPGGDPTVPGLPGLPDLGGVVGGVVDTIGGLIPGLGELPGLPGGDP
                     TVPGLPGLPGLPDLGGVVGGVVDTIGGLIPGLGELPGLPGSDPTVPGLPGLPGLPDLG
                     GVVGGVVDTIGGLIPGLGELPGLPGGDPTVPGLPGLPDLGGVVGGVVDTIGGLVPGLP
                     ELPGLPGGDPTVPGLPDLGGAVGGVVDTIGGLIPGLGELPGLPGGDPTLPGLPGLPDL
                     GGLIPGLPGLPDLGGVVDTIGGLVPGLGELPGLPGGDPTVPGLPGLPDLGGVVGGVVD
                     TIGGLVPGLGELPGLPGGDPTVPGLPGLPDLGGIVGGVVDTIGGLVPGLPELPGLPGG
                     DPTVPGLPGLPGLPDMGGVVGGVVDTIGGLVPGLPELPGLPGGDPTVPGLPGLPDLGG
                     LIPGLPGLPDLGGVVGGVVDTIGGLIPGLGELPGLPGGDPTVPGLPGLPGLPDLGGVV
                     GGVVDTIGGLVPGLGELPGLPGGDPTVPGLPGLPDLGGAVGGVVDTIGGLIPGLGELP
                     GLPGGDDPTVPGLPGLGGVVDTIGGLIPGLGELPGLPGGDPTVPGLPGLGVIGGVVDG
                     VGDAAGQLPLVGGLVGEVVDTVGGLVPGLDAGEPGTPGTPGTPGTPGTPGTPGTPGTP
                     GTPGTPGNPGTPGSTSGATHAAAAVVHGVSRSGSNSQGGAATLASTGSEPSILGLAAL
                     LFLMLGSLMIARPRQQD"
     gene            complement(8711..9931)
                     /locus_tag="VN041_09765"
     CDS             complement(8711..9931)
                     /locus_tag="VN041_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29362.1"
                     /translation="MRSAFADYRSARVWLLCGFGALLWAALTVLLGGSVAHAASDSPH
                     PPQSASSSAAQHPLHGASGSHVMDAPTQVPLPAGAADIVQQVREQVADIVQQSPQSEP
                     AAAQVHQPAASAQKPAQAAAQAQKPTQTQPSQLREQTVQAPVRDQAPARLQKATAALP
                     ASLQRLTAAVETSDTTVAFSTASVQTLSFAQMSAFTSPSDFSGESADFAMESGDFAME
                     SGDFAMDSYRVAVQQASDDFADAAELFASTAVSTHQHGNQALAHAVKTVRAALKGQFD
                     QARGDRGSRGDRAPIAAVNPKAVKAHIILAPRVTHAATSSNTVATPVAVSAAPAPQTP
                     QGPPAHLDVTPRHPLSAPPEASSAGSGGGMSAGTLPADAGIDDTLGMVTRAMRAGHEL
                     PPAGPVGSTDVSPD"
     gene            10430..11824
                     /gene="dnaB"
                     /locus_tag="VN041_09770"
     CDS             10430..11824
                     /gene="dnaB"
                     /locus_tag="VN041_09770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWU29363.1"
                     /translation="MSIADISDERLGGSRPPERTPPHDLLAEQSALGGMMLSKDAVAD
                     VIETLKGADFYVPKHELIFEAILSLYSHGEPTDVVAVTDELIKTGELGRAGGADYLHT
                     LTSIVPTAANAGYYAGIVSERAILRRLVDAGTRIVQLGYAGEGDATDIVNNAQAEIYS
                     VTGSETAEDYVPLTIAVDAAVEEIEAANGRDGTMTGIPTGFRELDELTNGLHGGQMIV
                     IAARPAMGKSTLALDFARAASIGHNEPSIFFSLEMGRSEIAMRLLSAEGAIPLQNMRK
                     GTLDPRDWTTVAATRGRINDAPLYIDDSPNMTLVEIRAKCRRLKQRAGLRLVVIDYLQ
                     LMTSGKRVESRQQEVSEFSRALKLLAKELQVPVIALSQLNRGAEQRSDKKPAISDLRE
                     SGSIEQDADMVILLHREAAYDKDVRPGEADLIVAKHRNGPTATITVAFQGHYSRFADM
                     APGDFGGGGGGDFN"
     gene            11965..12474
                     /locus_tag="VN041_09775"
     CDS             11965..12474
                     /locus_tag="VN041_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29364.1"
                     /translation="MTGLLLASAVALLLAGCTGMTPGAMAPAGAPNASPTPTRACPQI
                     DGADLPPECVPYDPDNAMAQNERYRDRMEMSAASRAAAEEAVVALRPALEQLRASGEV
                     SPDFVQAALQDAGLDGIEMIGDERGTEFSANGPEGGCIFGVVSDDPLTVEVGGYVMDG
                     GCFTPRGGH"
     gene            complement(12492..>13085)
                     /locus_tag="VN041_09780"
     CDS             complement(12492..>13085)
                     /locus_tag="VN041_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29365.1"
                     /translation="SDDRPGSGPDDEAADLAAVIDAAGGADAVLGHSSGAVLALYAAS
                     RGVATGALFLSEPPIDFDGSGFGEELPDKLQALVDAGTPEEAVASFQLDAVGLPAAMV
                     EAERGNGQLAALAPLGQSTVYDARLTLGLRQPDATLLDVAPPVTVLRGEQTFPFLIGS
                     SDRLAAEIPKAELVIVPESVMHRPDPAATAKVIAERL"
BASE COUNT         1740 a         4257 c         4778 g         2310 t
ORIGIN      
        1 gtccgggggc tcgggcacgt ccgcaggcgg ctccggcgcg gccggggcat ccatgatctc
       61 ggatgcggcg gccgctgcgt cccttcacga ggccacggcc tctctggcgc tgagcaccgt
      121 gcacgacgaa ctgccttcgt cgcccgtgtt cgacaccgac tccacccccg actgagcgag
      181 ggagcgtcgc gctgcgtgcg cgatgtgcgt gattcatcag atgtgaatcc actcttcttc
      241 gtactcagaa agggttaggc atgaacaagt acgtttcgcg cggtctgctt ctcgcaggct
      301 tcgcaggcgg cctctgggtc gcaggtactg gtgtcgctgc cgccgctgaa acacctgccg
      361 gggatgaagc cccggcggtc gtcaatgtcc agctgcctct cacgctcgag ggtcaggccg
      421 tctccgcgac gggcacgtcc actcaggacc cgcccgccga gaagccgaag cctgctccga
      481 tcgtgtccgt cgacctcaag gccgtaacag gcgtcttgaa gggcgagaag gcggtcgccg
      541 ctgtctcgac gccggtgacg gtgtcgggta acgcggtgag cgtgaccggg aacgcgaaat
      601 cgacggcttc gtcggtcacg caggctccgg cgcagccgaa ggacgccccg agctcgaacg
      661 atccgctggg agtgctcacg ggcgcccagg gactcgtcga cgtctccgcg ccggtgacgg
      721 tggcggggaa cgcggtgagc gtgttcggcg atgcggaatc gcacggctcg gcagtgacga
      781 ccacaggtgc aggcggcgat ggcgacggca gcgctctcga gaatgagggc atcggcgtgg
      841 ggacgggctg gcaggctgcc ggcgacgtga ccgcgccgct gaccgcggcg ggcaacgccg
      901 cgagcgtctt cggggatgcg tcctcgaacg actcgcgcgt cgatgtgcgc gacgctgacg
      961 gcaccgccgg cggtgcgggc acgatctcga actcggggat cgggctcaac accggatggc
     1021 aggtgcccgc tgatgtgacg acgccgctca cggcggccgg caacgccggc tccctgttcg
     1081 acgacgctgc atcgaacggt tcccaggtcg cgatcgagga tcgttccgga gtcgcgaact
     1141 ccggcggtcg gatcgagaac tcggggatcg gcaaggacac gggatggcag atccccgcgg
     1201 atgtcgtcgc tccggtgacg ttctcgggca acgcgatctc gggcacgggt gaggcagtca
     1261 cctcgggctc ggatgtcgcg gtctccgacc gggcgggtgt cgcgaacggc ggcggcgagg
     1321 tgtcgaacac aggcatcggc tggctcgtcg gatggcagat ccccgctgac gtgtccgctc
     1381 cgctgacggc atccggcaac gccttctcgg cgacgggtga tgtggcgagc cgggattcgg
     1441 gcgtgacggt ggccgactcg gccggcgtcg cgaacagcgg cggcgaggtc ggcaacgccg
     1501 gctacggcgt cggatccggc tggcagatcc ccgccgacgt gatcgctccc gtgaccgtgt
     1561 ccggcaacgc cggatcggtg ctcggcgacg cgggaacgtc tggatcggat gtcgcgatcg
     1621 tagaccgctc cggtgtggcg agcggcaacg gcgaggtgac gaatgcaggg tacggtgtcg
     1681 gcgctggctg gcagatccct gcggatgtca ccgctccgct gacgttctcc ggcaacgcgg
     1741 tatccggttc ctgtgacgca gtcacgacgg gctcggatgt ctcggtgctc gacaacgccg
     1801 gtgtcgccga cggcggcgcg gcctccaacg ccggttacgg tgtcggggcc ggctggcaga
     1861 tccccgcgga tgtgacgatg ccggtgacgg cgtcgggcaa cgcaggggcg ctgctggaca
     1921 acgccacctc gaccggttcc ggggtcgcga tcgtcgacaa cggcggcgtg gccgacggtg
     1981 gcagcgagat cggcaacgcc ggcttcggac tcaactcggg ttggcagatc ccggccgacg
     2041 tcaccgctcc ggtgacgctc gccggcaacg ccgtctcggc ggggaacgag gccgtcacga
     2101 ccggttccgg tgtcggcatc gtcgatgacg cgagcgtcgc cgacggttcg ggtgaggtct
     2161 ccaacgtcgg tgtcggaaag gacaccggct ggcagatcgt caccgactgg accacgccgc
     2221 tgacggcatc gggcaacgcc gtctcgggtg cgggtgacgc ggtcaccacg gactcgggtg
     2281 tcggcatcgt cgaccgcggc gacgcggtgg acggcgcagg cgaggcgtcc aacacgggca
     2341 tcggctggct cgtcggctgg caggtgctcg gcgatgtgac cgctccggcc acggcatccg
     2401 gaaactccgg ttccgtgctc ggggatgccg gtaccaccgg ctccacggtc ggcgtcgtcg
     2461 acaacgccgg cgtcgtgaac ggcgacggtg acgtctcgaa cgcagggtac ggtgtcggcg
     2521 caggctggca ggtgctcggc gacgtgacgg cgccggtgtc gttctcgggc aacgcggtct
     2581 cgggcagtgg tgactcgctc accaccgact cgggtgtcgc ggtggtcgac gacgcgggcg
     2641 tgatcgacgg cgacggcgac gtctccaaca cgggcatcgg cctcaactcc ggttggcagg
     2701 tcgtgacgga ttggacgacg ccgggtacgg catccgggaa cgcgatctct ggtgtgggtg
     2761 acgccagcac cgcgggctcg gatgtcgccg tggtcgaccg tgcaggcgtc atcgacggct
     2821 ccggcgtgat ctcgaacgac ggcatcggca aggactcggg ctggcaggtc gccggcgacg
     2881 tgaccgtgcc gctgacggcc tcgggcaacg ccggctccgc tttcggtgat gcggatgctg
     2941 tcgattcggg cgtcggcgtc gtcgacgaca ccgctgtgat cggctctgcc ggtcaggcga
     3001 ccgacaacgg ggtgggcttc ggcaccggct ggcaggtcgt gggcgatgtc gtcgtgccgg
     3061 tgaccggttc gggcaacgcg gcgtcgggct tcggcgacgt ggtgaccgac ggctcggagg
     3121 tggccgtgct gaccgagggc ggttcgatcg tctccggcca cgcggacgac tccggcatcg
     3181 gcgtcgcctc cgggtggcag gtcgtgggcg gcgtggtcgc tccgatcacc gcctcgggcg
     3241 acgcggtcac ggtcgcgggt gacaccgggt cgcacgactc gctcgtcacc gtgcacggct
     3301 cgaacggcga cctcggcgac ggggctctcg gcaacgaggg cgtcggagtc ggaaccgcct
     3361 ggcagcccgt cgtcgagatc acgactccgg tcacggtctc gggcgatgcg gtcgctccgg
     3421 tgggcgcggt gtcgggtgac tccactgtcg tggtcgagcc cgggatgtcc ggttcgctga
     3481 acccggggct tcccggtgtt ccgtcgattc cggttcccga cctcggcgac gcagcgggcg
     3541 atgccctcgg caacgtgcac ggtctgccct cactgcccgg tggcgagctg gtgggcggtg
     3601 tgatcggtga cgtgatcggc gccggtgagc ccggagaccc ggctgttccg ggtgccggag
     3661 acctcatcga cggcgtgatc ggcgctgttc ccggcctgtc caccgcgccc ggcgtgccgg
     3721 agctcggagg ctcgctgggc gacgcggtcg actcgatcgg caccgtcatc ggcggcggtc
     3781 tgcccgaggt gcccacggag ccttcggttc cgggggagcc ggaggtcccg tcgctgcccg
     3841 tcatcggcgc cctcccgggc gtcggagagg tcgtcggcac ccttcccggt gtcggtggcg
     3901 tgatcgggag ccttcccggg attccggctc tgccggttct tccgggggag ccccacggtc
     3961 ccgtcctcga cctcggcgac ctggtcggtg acatcaccga ctgcccgtgc gacgcgatcg
     4021 gcggtgtcgt cgacaccgtc ggcgacctcg gcggaacgat cggtgatgtc gccggctcca
     4081 tcggcgacat cgccggtggg ctgcctgggc tgcctggtgg tgacccttcg gttccgggtc
     4141 ttccgggtct gccgggtctg cctgacctgg gcggggttgt cggtgatgtc gttgacaccg
     4201 tcggtggcct ggttccgggt cttccggagc tgccgggtct tcccggcggt gaccccacgg
     4261 ttcccggtct cccgggcctt ccgggtctgc cggacctggg tgggatcgtc ggtggtgtcg
     4321 ttgacaccat cggtggtctg attcctggtc tgggtgagct gccgggtctt cccggtggcg
     4381 accccacggt tccggggctt ccgggtcttc ccgggcttcc gggcctgcct gacctgggtg
     4441 gggttgtcgg tggtgtcgtt gacacgatcg gtggtctggt cccgggtctt ccggagctgc
     4501 cgggtcttcc cggtggtgac cccacggttc cgggtctccc cgggcttccg ggtctgcctg
     4561 acctgggtgg ggtcgttggt ggtgtcgttg acaccattgg cggtctggtt cctggtctgg
     4621 gtgagctgcc gggtcttccc ggtggcgacc ccaccgtccc gggtctcccg ggtctgcctg
     4681 acctgggcgg ggtcgttggt ggtgtcgttg acaccattgg cggtctggtt cctggtctgg
     4741 gtgagctgcc gggtcttccc ggcggtgacc ccacggtccc gggtcttccc gggcttccgg
     4801 gcctgccgga cctgggtggg gttgtcggtg gtgtcgttga cacgatcggt ggtctggtcc
     4861 cgggtcttcc ggagctgccg ggtcttcccg gcggtgaccc cacggttccg ggtctcccgg
     4921 gccttccggg tctgccggac ctgggcgggg tcgtcggtgg tgtcgttgac accattggcg
     4981 gtctgattcc tggtctgggt gagctgccgg gtcttcccgg tggcgacccc accgtcccgg
     5041 gtctcccggg gcttccgggc ctgcctgacc tgggcggggt cgtcggtggt gtcgttgaca
     5101 ccatcggcgg tctggtccct ggtctgggtg aactgccggg tcttcccggt ggtgacccca
     5161 ccgttcccgg gcttccgggt cttcccgacc tgggtggact cattccgggt cttccgggtc
     5221 tgcctgacct gggtggggtc gttggtggtg tcgttgacac cgttggtggt ctgattcctg
     5281 gtctgggtga gctgccgggt cttcccggtg gtgaccctac ggttccgggt ctgccgggtc
     5341 ttcctgacct gggtggggtt gtcggtggtg tcgtcgacac cattggcggc ctgatcccgg
     5401 gcctgggtga actgccgggt cttcccggcg gtgaccccac ggtcccgggt ctccccgggc
     5461 ttccgggtct gcctgacctg ggtggggttg tcggtggtgt cgttgacacg atcggcggtc
     5521 tgattccggg gctaggtgaa ctgccgggtc ttcccggcag tgaccccacg gtcccgggtc
     5581 tcccgggcct tccgggtctg cctgacctgg gtggggtcgt cggtggtgtc gttgacacca
     5641 ttggcggtct gattcctggt ctgggtgagc tgccgggtct tcccggtggc gaccccaccg
     5701 tcccgggtct cccgggtctg cctgacctgg gcggggtcgt cggtggtgtc gtcgacacga
     5761 tcggtggcct ggttccgggg cttccggagc tgccgggtct tcccggtggc gaccctacgg
     5821 ttccgggtct tcctgacctg ggcggggcag tcggtggtgt tgttgacacg atcggtggtc
     5881 tgattcctgg tctgggtgag ttgccgggtc ttcccggtgg tgaccccacc cttcccggtc
     5941 ttccgggtct tcccgacctg ggtggactca ttccgggtct gcctggtctg cccgatctcg
     6001 gtggtgtcgt tgacacgatc ggtggcctgg tccctggtct gggtgagctg ccgggtcttc
     6061 ccggtggtga ccccaccgtt cccggtcttc cgggtcttcc cgacctgggc ggggtcgttg
     6121 gtggtgtcgt cgacaccatc ggtggtctgg ttcctggtct gggtgagctg ccgggtcttc
     6181 ccggtggtga ccccaccgtc ccgggtctcc cgggtctgcc tgacctgggt gggatcgtcg
     6241 gtggtgttgt tgacaccatt ggtggtctgg tcccgggtct tccggagctg ccgggtcttc
     6301 ccggtggtga ccctacggtt ccgggtcttc ccgggcttcc gggcctgcct gacatgggcg
     6361 gggtcgtcgg tggtgtcgtt gacaccatcg gtggcctggt cccgggtctt ccggagctgc
     6421 cgggtcttcc cggtggtgac cccaccgttc ccgggcttcc gggtcttccc gacctgggtg
     6481 gactcattcc gggtcttccg ggtctgcctg acctgggtgg ggtcgttggt ggtgtcgttg
     6541 acaccattgg tggtctgatt cctggtctgg gtgagctgcc gggtcttccc ggtggtgacc
     6601 ccacggtccc gggtctcccc gggcttccgg gcctgcctga cctgggtggg gttgtcggtg
     6661 gtgtcgttga cacgatcggt ggcctggttc cgggtctggg tgagctgccg ggtcttcccg
     6721 gcggtgaccc caccgtcccg ggtcttccgg gtctgcctga cctgggcggg gcagtcggtg
     6781 gtgtcgtcga caccatcggt ggcctgattc ctggtctggg tgagctgccg gggcttcccg
     6841 gtggcgacga ccctacggtc ccgggtctgc ctggcctcgg tggtgtcgtt gacacgatcg
     6901 gcggcctgat cccgggtctg ggcgagctgc cggggcttcc cggtggcgac cccaccgttc
     6961 ccggccttcc ggggctggga gtgatcggtg gcgttgtcga cggtgtcggc gatgctgctg
     7021 gtcagttgcc tctcgtcggt ggtcttgtgg gggaggtcgt cgacactgtc ggcggcctcg
     7081 tccccggcct cgacgcgggt gagcccggaa cccctggtac gccgggtacg ccgggcacgc
     7141 cgggtacgcc gggtacgccg ggtacgccgg gtacgccggg cacgcctggt acgcctggta
     7201 acccgggtac gccgggctcg acgagtggcg cgacgcacgc ggctgcggcc gttgtgcacg
     7261 gcgtgtcccg ctcgggttcg aactcgcagg gcggcgctgc caccctcgcg agcacgggga
     7321 gcgagccgtc catcctcgga ctggccgctc tgctgttcct gatgctcggc tcgctcatga
     7381 tcgcgaggcc gcgccagcag gactgatcgg agggaatgac ggccgtggcc gcgcaagggg
     7441 gaggaagcgt gccacggccg tcatcagttt gcctacggga gatctgacat cccataggag
     7501 aacccgagtc ttgacggtgc ctttcccgtc gcgcccgcgc agatcgggcg tcgcgcgctc
     7561 cagtgcgcct cagcatccgt gtgtcacagc tctgcggacg gctgcagcag cgctgtgcgt
     7621 tgatcacgca cacgcgcgct gaccgaagca cgcgcggagc gtcgtgagcc tcgacgtcgg
     7681 tgctgcagcg caacgtcagc gcggtggcag cgaacgagtc accgaggccc cgcgtcgtga
     7741 tgatgtgcgg tccaggcgga gcaggcaaga ccacgtacgc gaagcgcctg gagagcgagg
     7801 gctggactcg cctgtcattc gaggtggagt tctgggatcg cgggatcacg acgatgcctt
     7861 ccccccgagt ggtggccgac gtgtcggcag aattgaaggt gcgcctgctg caccacgtcg
     7921 cggtggggaa cgacgtcgtg ctcgattcgg gttttggttc cggcggcaac gcgacgagta
     7981 tcgtgcgctg ctcgctcacc agggcatcgt gccggagacg gtctacatcg cgacgagtct
     8041 cgagaccatc ctgagccgag ccggtgaccg gcatggcagg tgggctgacg actggccgct
     8101 caccgagcag acggcggtcg agtacttcgc gaagttcgag ccccgacgcc cgatgagggt
     8161 cccctcgagg tggtcgatta ggcccgtcct gggcgttcac gccccggcgc gagcgacaac
     8221 cgctcaaggc gatagaggcg ctccatgccc gtcgcagcac gtcgacgatg ctccggattt
     8281 ctcgaaggac tcaccctgcc gggcggagag aagaaggggc gggtgcctca ccatgaggga
     8341 gtgaggcacc cgcctgagcg gccggcactg gaaggccggt gaccgctcgg tttgcgacta
     8401 cgagggggag gggatcccct cgaagtcgct caaggatgcc gtgccgcgcg gggcgctggg
     8461 attcgccgct gggcgcaacg tggcatccga tccgtcagcc gatcgccttc ccccgaaggc
     8521 gatgcagatg ccgacggaag tcgtcatgcc gtgcggaacc ggctgtcgtc gctgaggacg
     8581 ctgcgcattg tcgggtctcc tgaccagatc gatggatcac gtgcggacgc gttcgaagcc
     8641 gcgtcggatg cgacggctca gagcctctga aagacagagt gctgccgtcg agaaagcgca
     8701 gtggtcctga tcagtcaggg gagacgtcgg tggaaccgac gggtccggcc ggcggaagct
     8761 cgtgcccggc gcgcatcgcg cgagtgacca tccccagcgt gtcgtcgata ccggcgtctg
     8821 cgggaagggt gcctgcagac atccctccgc cggatccggc ggacgatgcc tccggcgggg
     8881 cggagagcgg atgccgagga gtgacgtcca ggtgcgcagg cggtccctgg ggcgtctgcg
     8941 gcgcgggagc ggcagaaact gcgaccgggg tcgcaaccgt gttcgacgaa gtggcggcgt
     9001 gggtgacccg cggcgccaag atgatgtgcg ccttcacggc cttcgggttc acagccgcaa
     9061 tgggcgcacg gtcaccacga gatccacggt ctccccgggc ctggtcgaac tggcccttca
     9121 gagccgcgcg gaccgtcttc accgcgtgag ccagcgcctg attgccgtgc tggtgggtgc
     9181 tgaccgccgt ggacgcgaag agctccgcag catccgcgaa gtcatcggaa gcctgctgca
     9241 ccgcgacccg gtaagaatcc atcgcgaagt ccccagactc catcgcgaag tccccagact
     9301 ccatcgcgaa gtccgcagac tcacccgaga agtccgaggg cgaggtgaac gccgacatct
     9361 gcgcgaacga cagagtctgc acggacgcgg tggagaaggc caccgtggtg tccgaggtct
     9421 caacggcggc tgtcagccgc tgcagggacg ccgggagcgc cgcagtggcc ttctggagcc
     9481 tggccggcgc ctgatcgcgc acgggagcct gcacagtctg ctcgcgcagc tgcgacggct
     9541 gagtctgggt gggcttctga gcctgggcgg cggcctgagc cggcttctgc gcggacgccg
     9601 ccggctggtg cacctgcgca gctgcgggct cgctctgggg cgactgctgc acgatgtccg
     9661 cgacctgctc gcggacctgc tgcacgatgt ccgcggcccc tgcggggagc ggcacctgcg
     9721 tgggcgcatc catcacgtgc gaaccggatg cgccgtgcag cggatgctgg gcggccgagg
     9781 acgatgcgga ctgcggcgga tgcggcgaat cagaggctgc gtgggccacg gaaccgccga
     9841 gaagcacggt cagagcggcc cacaagagcg cgccgaaacc gcacagcagc catactcggg
     9901 cgctgcggta gtcggcgaac gctgaccgca cgtgattcct tccccggaac gagccgcgcc
     9961 gatatttcgc gcaacacgat cgctgtgacg ataccccgtg cgagggcgga tgtccacacg
    10021 ggaggagatc ctctacaaac ccgccgcgcg cgggaagatc aagacttgac tttccccagg
    10081 ttctgcacat ccccgtaacc agccgtcaac cttaaatctg gcattgaagt cgaatctcat
    10141 ccacaagggt gtgaatcaag atatcccagg tcagatgtct tattcgactc taaagatgct
    10201 tctctccacc gagttctcca caggggttct gcacagacac tcccgagttg tccgcacact
    10261 ctccacaaag ttatccacag ggtgtcttgc atggggatgg agcggctcct aacttggtgg
    10321 agaccggatg tcggtgactc gtggttcgct gtgcgagcgg gccggatcca ctccggtcgt
    10381 gtttgccgag aaggtgccat cagtcgccac cggcggggga ggagcacgag tgtcgatcgc
    10441 ggatatctct gacgagcgtc taggcgggtc gcgcccgcct gagcggaccc ctccgcacga
    10501 cctgctcgcc gagcagagcg cgctgggcgg catgatgctc tcgaaggacg ccgtcgccga
    10561 tgtcatcgag acgctcaaag gcgcagactt ctacgtcccc aagcacgagc tgatcttcga
    10621 ggccatcctc tcgctgtact cgcacggcga gccgacggat gtcgtggccg tcaccgacga
    10681 actgatcaag acgggcgagc tcggcagggc cggaggtgcc gactacctgc acaccctcac
    10741 ctcgatcgtg cccaccgcgg cgaacgcagg ctattacgcg ggcatcgtct cggagcgtgc
    10801 gatcctgcgt cgtctggtgg atgccggcac gcgcatcgtg cagctcggct acgcgggtga
    10861 aggcgatgcg acggacatcg tcaacaacgc ccaggccgag atctactcgg tcaccgggtc
    10921 cgagaccgcc gaggactacg tgcctctcac gatcgccgtt gacgcggcgg tcgaggagat
    10981 cgaggcggcc aacggccgtg acgggaccat gaccggcatc ccgaccggct tccgcgaact
    11041 cgatgagctg accaacggac tgcacggcgg tcagatgatc gtcatcgccg cacgtcctgc
    11101 catgggtaag tccacgctcg ccctcgactt cgcgcgcgcg gcatccatcg gtcacaacga
    11161 gccgtcgatc ttcttctcgc tcgagatggg gcgcagcgag atcgcgatgc gcctgctcag
    11221 tgctgaaggc gcgatcccgc tgcagaatat gcgcaagggc acgctcgacc cgcgcgactg
    11281 gacgacggtc gccgctaccc gcgggcgcat caacgacgcg ccgctgtaca tcgacgacag
    11341 ccccaacatg acgctggtcg agatccgggc caagtgccgt cggctcaagc agcgcgcggg
    11401 cctgcgactg gtggtcatcg actacctgca gctgatgacc agcggcaagc gcgtcgaatc
    11461 gcgtcagcag gaggtctcgg agttctcgcg tgcgctgaag ctgctcgcca aggagctcca
    11521 ggtaccggtg atcgcgctct cgcagctgaa ccgtggtgcc gagcagcgct ccgacaagaa
    11581 gccggcgatc agcgaccttc gtgagtccgg ctcgatcgag caggacgccg acatggtgat
    11641 cctgctgcat cgcgaggccg cgtacgacaa ggacgtgcgc cccggcgagg ctgacctgat
    11701 cgtcgccaag caccgtaacg gccccaccgc aaccatcacc gtcgcgttcc agggtcacta
    11761 ctcccgcttc gcggacatgg cccccggcga cttcggcggc ggtggcggcg gcgacttcaa
    11821 ctgagtcgag cgtccgctct gcggatcgtc ggtaccggcg tcgtcacgct cgtctgcgac
    11881 gtgcagcact gatccgatcg cggccggggg ttccggcgcc cgagctgctg tgctctgatg
    11941 gagggcatgg ccgccacgag ggggatgacg gggctgctgc tggcgagcgc agtcgccctg
    12001 ctgcttgccg gttgcacggg gatgacaccc ggcgcaatgg cgcccgctgg tgcgccgaat
    12061 gcctcgccaa ccccgacgcg agcatgcccg cagatcgacg gagctgacct gccgcccgaa
    12121 tgcgtgccct acgacccgga caacgcgatg gcgcagaacg agcgctaccg cgaccggatg
    12181 gagatgtcgg ccgcgagccg ggctgcggcc gaagaggcgg tcgtcgccct acgccctgcg
    12241 ctcgaacagt tgcgcgcgtc gggtgaggtc tcacccgact tcgtgcaggc tgcgctgcag
    12301 gatgccgggc tcgacggcat cgagatgatc ggcgacgaac gcgggacgga gttcagcgcg
    12361 aacggacccg agggtggctg catcttcggc gtggtgtcgg acgacccgct caccgtcgag
    12421 gtcggcgggt acgtcatgga cggcggctgc ttcacgccgc ggggcgggca ctgagcccga
    12481 acccgacggt ctcagagccg ctccgcgatc accttcgctg tcgccgcggg gtcgggccgg
    12541 tgcatgaccg actcggggac gatcacgagt tcggccttcg gaatctccgc cgccagccga
    12601 tccgacgagc cgatgagaaa cgggaacgtc tgctcgccgc gcagcacggt gaccggcggc
    12661 gccacgtcga gcagggtcgc gtccggctgc cgcagcccca gagtgagccg cgcgtcgtac
    12721 accgtcgact gacccagggg cgcgagcgcc gcgagctgac cgttcccccg ctccgcctcg
    12781 accatggcag ccgggaggcc gacggcatcc agctggaagg aggcgaccgc ctcctccggc
    12841 gtgcccgcgt cgacgagcgc ctgcagcttg tccggcagct cttccccgaa acccgagccg
    12901 tcgaagtcga tcggcggttc ggagaggaac agtgcaccgg tcgccacgcc ccgggatgcg
    12961 gcgtacagcg cgagcaccgc gcccgaggag tggccgagca cggcatccgc tcctcctgcc
    13021 gcatcgatga cggcggcgag atccgcggct tcgtcgtcgg ggccggatcc cggccggtcg
    13081 tcgct
//
LOCUS       DATMSX010000319         5694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_80490,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000319 DATMSX010000000
VERSION     DATMSX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80490"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1296
                     /locus_tag="VN041_09785"
     CDS             22..1296
                     /locus_tag="VN041_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29366.1"
                     /translation="MAQPGPGEHPKQPEPRSAVFVRELMRGSVVTTILAIVAGFVIGG
                     ILIVVTNPDVQAAAGYFFQRPSDTFAAIWQALSGGYGALFQGSIVNFNATDFAGFIMP
                     LTNTLKFAAPLIMAGLGVGLAFRVGLFNIGGQGQILIAGAFAGFLSYQLPFDMPMWLH
                     IPYSIVIGMIGGAIWAGIAGLLKARTGAHEVILTIMLNWIALYLIGYLVRTPGLQKPG
                     SNQSQSVPTPESAQFPPLLGPQFPGLTVGFLVAILLAIAVWWLMERSGLGFRMRAVGE
                     NPSAARSAGISVERMYTYAMIFAGALAGAAGINQISTATVDGFDSTFDSGIGFDAITV
                     ALLGRSRAGGIFAAGLLFGALKAGSFAMSGQGIPKEIVVVVQSIIVLFIAAPPLVRSM
                     FFLPKSEAERAAKAKAKADKKAARAAAKEVAA"
     gene            1293..2570
                     /locus_tag="VN041_09790"
     CDS             1293..2570
                     /locus_tag="VN041_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29367.1"
                     /translation="MTAIAEAPVARTVVTPVAKRLPISLSVATVLLLALFLLAPRHGT
                     AVFRLDDGTTALKIPDQSVSADLVVVLVSILLVLLSAWSWIAAVRRIRAGAWVPIVFA
                     ILGVFAFVTWAAADANGRVPVVNMLYGAISLAVPLAFGALGGVVGERAGVVNVAIEGQ
                     LLLGAFLGAVFGTLAHNPLFGLVGAIIGGMLVAFVLAAFAIKYLVEQVIVGVVLNVLV
                     SGLTTFLFTILLVPNSDTLNTTPQFGKIKIPLLSEIPVLGPVLFNQNLIVYLLYIAVP
                     VVAFGLYRTRWGLRLRAVGEYPRAADTVGIKVNPTRFWNLMLAGALAGIGGAYFTLVS
                     VPHFNRDMTNGAGFIALAAVIFGRWDPVRATLAALLFGFATNLQNTFGAIGTPVPGEF
                     LLMLPYVVTLIAVVGFVGQIKAPAADGKPYIKS"
     gene            2613..3011
                     /locus_tag="VN041_09795"
     CDS             2613..3011
                     /locus_tag="VN041_09795"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWU29368.1"
                     /translation="MTDIDWDDLRQVATEAMQKAYAPYSRYRVGAAALVDDGRVVAGC
                     NVENASYGVTLCAECALVGDLHMSGGGKLVAFVCVNNDGQTIMPCGRCRQLLSEHASP
                     EMLLETVSGIRTIDEVLPDRFGPADLEIVR"
     gene            3008..4300
                     /locus_tag="VN041_09800"
     CDS             3008..4300
                     /locus_tag="VN041_09800"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HWU29369.1"
                     /translation="MSVEPFDAVDVIRTKRDGGAVAEAPLRWMIDAYTRGYVSDAQMA
                     SFAMAVLQRGMERDEIRVMTDAMIATGERMSFADLGKRTVDKHSTGGVGDKITLPLAP
                     LVASFGVAVPQLSGRGLGHTGGTLDKLESIPGWRAALSNEEMFAQMKGDVGAVICAAG
                     SGLAPADKKLYALRDVTGTVEAIPLIASSIMSKKIAEGTDALVLDVKFGNGAFMQDID
                     RARELARTMVALGTDSGVATTALLTDMNTPLGLAIGNANEVRESVEVLAGGGPADVRE
                     LTLALAREMLALAGQPDADVEKALDDGRAMDSWNAMIRAQDGDPDAALPTARETHVVR
                     AAVDGVVTRMDALPFGIAAWRLGAGRARAEDPVIHAAGIDLAVKPGDRVTAGQTLFTL
                     SADDDARFARALQALDGAYEVGQSASAPQPIVRERITA"
     gene            4320..5435
                     /locus_tag="VN041_09805"
     CDS             4320..5435
                     /locus_tag="VN041_09805"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWU29370.1"
                     /translation="MTIDQNGDLTVQGRSLRALPKVSLHDHLDGGVRIGTILELAEEA
                     GVTAPAPDAKGLRRWFDQQCGTGSLPQYLKTFDLVTSVLQTERALTRVAREFVADLAA
                     DGVVYGEVRWAPEQHLTGGLSLDAAVQAVQAGIEQGEDDADAAGRSIRVGQILSAMRQ
                     EGRAREIAELAVRFRERGVVAFDIAGPEDGFPASRHREAFDYLASEFMPVTVHAGEAA
                     GPGSIRSALIDGRAVRLGHGVRIAEDLQIIEREGDDVQVQFGDLARWVRDREIPLELS
                     PSSNLQTGAIAPWGDEFADHPFDLLYQLGFSVTVNTDNRTMSGTSLTRELALLVDAFD
                     YDLDDLEEFQFNAASAAFLPVEEREELVEMIGEGFER"
     gene            5443..>5694
                     /locus_tag="VN041_09810"
     CDS             5443..>5694
                     /locus_tag="VN041_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWU29371.1"
                     /translation="MSSVFIAGPASWNQLVVLDRLPEPVPHMQFAEDSWETLGGTSAG
                     KALSLTALGRETLLYAVTADDEPGRRVRVALERAGVATRW"
BASE COUNT          807 a         2000 c         1979 g          908 t
ORIGIN      
        1 gcggatgcgg cggccgcgca gatcgcgcag cccggacccg gcgagcaccc caagcagccc
       61 gagccgcgct cggcggtctt cgtgcgagag ctgatgcgcg gcagcgtcgt gacgacgatc
      121 ctcgcgatcg tcgccggctt cgtgatcggc ggcatcctga tcgtcgtcac caaccctgac
      181 gtgcaggccg ccgccggcta cttcttccag cggccgtccg acacgttcgc cgcgatctgg
      241 caggcgctct ccggaggcta cggggcactg ttccagggct cgatcgtcaa cttcaacgcc
      301 accgacttcg ccggcttcat catgccgctg acgaacaccc tgaagttcgc cgcgccgctg
      361 atcatggcgg gcctcggcgt cggtctcgca ttccgcgtgg gcctgttcaa catcggcggt
      421 cagggtcaga tcctcatcgc gggcgcgttc gcgggcttcc tgtcctacca gctgcccttc
      481 gacatgccca tgtggctgca catcccgtac tcgatcgtca tcggcatgat cggcggcgcg
      541 atctgggcgg gcatcgcggg actgctgaag gcgcgcaccg gcgcgcacga ggtcatcctc
      601 acgatcatgc tgaactggat cgcgctctac ctgatcgggt acctggtgcg cacacccggg
      661 ctgcagaagc ccggctccaa ccagtcgcag tcggtcccga cgccggagtc cgcgcagttc
      721 ccgccgctgc tcggaccgca gttcccgggc ctcaccgtcg gcttcctggt ggccatcctg
      781 ctcgccatcg ccgtctggtg gctgatggag cggtccggtc tcggattccg gatgcgcgcg
      841 gtcggcgaga accccagcgc cgcacgctcc gccggcatca gcgtcgagcg gatgtacacc
      901 tacgcgatga tcttcgcggg tgcgctcgcc ggagccgccg gcatcaacca gatcagcacc
      961 gcgacggtgg acggcttcga cagcaccttc gactccggga tcggcttcga cgcgatcacg
     1021 gtcgccctgc tgggacgcag ccgcgccggc ggcatcttcg ccgctggtct gctcttcgga
     1081 gcgctgaagg ccggttcgtt cgcgatgtcc ggtcagggca tcccgaagga gatcgtggtc
     1141 gtcgtgcagt cgatcatcgt gctgttcatc gccgcgccgc cgctggtgcg ctcgatgttc
     1201 ttcctgccca agagcgaggc cgagcgcgcc gcgaaggcga aggcgaaggc agacaagaag
     1261 gccgcccgcg cggccgcgaa ggaggtggcg gcatgaccgc gatcgccgag gcgcccgtgg
     1321 cccgcaccgt ggtcacgccc gtcgccaagc gactgccgat cagcctgagc gtggccaccg
     1381 tgctgctgct cgcgctgttc ctgctcgccc cgcgtcacgg cacggccgtg ttccgtctcg
     1441 acgacggcac gaccgcgctg aagatccctg accagagcgt ctccgccgac ctggtggtcg
     1501 tgctggtctc gatcctgctg gtgctgctga gcgcctggtc gtggatcgcg gccgtccgcc
     1561 gcatccgagc cggcgcatgg gtgccgatcg tgttcgcgat cctcggtgtg ttcgccttcg
     1621 tcacctgggc ggcggcggat gccaacggcc gggtacccgt ggtgaacatg ctctacggtg
     1681 cgatctcgct cgcagtgccg ctcgcgttcg gcgccctcgg cggtgtcgtg ggcgagcgcg
     1741 ccggcgtcgt caacgtcgcg atcgaggggc agctgctgct cggcgccttc ctcggcgccg
     1801 tgttcggcac gctcgcgcac aacccgctgt tcggtctggt gggcgcgatc atcggcggca
     1861 tgctcgtggc gttcgtgctc gcggcgttcg cgatcaagta cctggtggag caggtgatcg
     1921 tcggcgtggt gctgaacgtg ctggtcagcg gtctgaccac gttcctgttc acgatcctgc
     1981 tggtccccaa ctccgacacc ctgaacacga cgccgcagtt cggcaagatc aagatcccgc
     2041 tgctcagcga gatccccgtg ctcggcccgg tactcttcaa ccagaacctg atcgtgtacc
     2101 tgctctacat cgcggtgccg gtcgtggcct tcggtcttta ccgcacccgc tggggactgc
     2161 ggctgcgcgc ggtcggcgag tacccgcgcg ccgccgacac ggtcggcatc aaggtgaatc
     2221 ccacccggtt ctggaacctg atgctcgccg gtgctctcgc cggcatcggc ggtgcgtact
     2281 tcaccctggt gtcggtgccg cacttcaacc gcgacatgac caacggcgcg ggcttcatcg
     2341 ccctggccgc cgtcatcttc ggccgctggg acccggtgcg cgccacgctg gccgcgctcc
     2401 tgttcggctt cgcgaccaac ctgcagaaca cgttcggcgc catcggcacg ccggtccccg
     2461 gcgagttcct gctgatgctg ccgtacgtcg tcacgctgat cgccgtggtc ggcttcgtcg
     2521 gccagatcaa ggcccccgcg gccgacggca agccgtacat caaatcctga cggcacgccg
     2581 tacatcgagc actgaggagc tgtgaagaag tcatgacgga catcgactgg gacgacctgc
     2641 gtcaggtcgc gaccgaagcg atgcagaagg cgtacgcgcc gtactcgcga taccgggtgg
     2701 gcgctgcggc gctcgtcgac gacgggcgcg ttgtcgccgg atgcaacgtc gagaacgcct
     2761 cctacggcgt caccctgtgc gcggagtgcg cactggtcgg cgatctgcac atgtccggcg
     2821 gcggcaagct cgtggccttc gtctgcgtga acaacgacgg gcagaccatc atgccctgcg
     2881 gtcgctgccg tcagctgctg tccgagcacg cgagcccgga gatgctgctg gagaccgtct
     2941 ccggcatccg cacgatcgac gaggtgctgc ccgacaggtt cggtcccgcc gacctggaga
     3001 tcgtccgatg agcgtcgagc cgttcgacgc cgtcgacgtc atccgcacca agcgcgacgg
     3061 cggcgcggtg gccgaggcgc cgctgcgctg gatgatcgac gcgtacaccc gtggctacgt
     3121 gtcggacgcc cagatggcgt cgttcgcgat ggccgtgctg cagcgcggca tggagcgcga
     3181 cgagatccgc gtgatgaccg acgcgatgat cgcgacgggg gagcggatga gcttcgccga
     3241 cctcggcaag cgcaccgtcg acaagcactc caccggcggc gtgggcgaca agatcacgct
     3301 tccgctggcg cccctggtgg catccttcgg cgtggccgtg ccgcagctga gcggacgcgg
     3361 cctggggcac accggcggca cgctcgacaa gctcgagtcg atccccggct ggcgtgctgc
     3421 gctgagcaac gaggagatgt tcgcccagat gaagggcgac gtcggcgcgg tcatctgcgc
     3481 ggcaggctcc ggccttgctc cggcggacaa gaagctgtac gcgctgcgcg acgtcaccgg
     3541 caccgtcgag gcgatcccgc tgatcgcctc gagcatcatg tcgaagaaga tcgccgaggg
     3601 cacggatgcc ctggtgctgg acgtgaagtt cggcaacggc gcgttcatgc aggacatcga
     3661 ccgcgcccgc gagctcgccc gcacgatggt cgccctcggc acggattcag gcgtggccac
     3721 cacggcgctg ctgaccgaca tgaacacccc gctgggcctc gcgatcggca acgcgaacga
     3781 ggtgcgcgaa tcggtggagg tgctcgccgg cggcggcccc gccgacgtgc gcgaactgac
     3841 gctcgccctg gccagggaga tgctcgccct ggcgggacag ccggatgccg acgtggagaa
     3901 ggccctcgac gacggacgcg cgatggactc ctggaacgcg atgatccgcg cgcaggacgg
     3961 cgatcccgac gcggcgctgc ccaccgcgcg tgagacccat gtggtccgtg ccgccgtcga
     4021 cggcgtcgtg acccggatgg acgcgctgcc cttcggcatc gcggcctggc gtctgggtgc
     4081 gggccgcgcc cgcgcggagg acccggtgat ccacgccgcc ggcatcgatc tggccgtcaa
     4141 gcccggagac cgcgtgacgg cggggcagac cctgttcacg ctctcggccg acgacgacgc
     4201 ccgcttcgcc cgcgcgctgc aggcgctcga cggcgcctac gaggtcggcc agtcggcgtc
     4261 cgccccgcag cccatcgtgc gcgagcgcat caccgcctga accccacgga gagatctcga
     4321 tgaccatcga ccagaacggc gatctgacgg tgcagggccg gtccctgcgc gcactgccca
     4381 aggtgtcgct gcacgaccac ctcgacgggg gcgtgcgcat cggcaccatc ctcgaactcg
     4441 cggaagaggc gggcgtcacg gctcccgcgc ccgatgccaa gggcctgcgg cgctggttcg
     4501 accagcagtg cggcacgggg tcgcttccgc agtatctgaa gaccttcgat ctggtcacct
     4561 cggtgctgca gaccgagcgc gccctgacgc gggtcgcacg ggagttcgtc gccgatctgg
     4621 cagcggacgg cgtcgtgtac ggcgaggtgc gctgggcccc tgagcagcac ctgaccgggg
     4681 gcctgagcct ggacgccgcc gtgcaggcgg tgcaggccgg catcgagcag ggcgaggacg
     4741 acgcggatgc cgccgggcgc agcatccgcg tcggtcagat cctctccgcc atgcggcagg
     4801 agggccgggc ccgtgagatc gccgagctgg cggtgcgctt ccgagagcgc ggcgtcgtcg
     4861 ccttcgacat cgccggtccc gaggacgggt tccccgcgtc acgtcaccgc gaggcgttcg
     4921 actacctggc atccgagttc atgccggtca cggtgcacgc cggtgaggcg gcgggccccg
     4981 gctccatccg ctcggcgctc atcgacggtc gcgcggtgcg gctgggccac ggcgtgcgca
     5041 tcgcggagga cctgcagatc atcgagcgcg agggcgacga cgtgcaggtg cagttcggtg
     5101 acctggctcg ctgggtgcgc gaccgcgaga tcccgctgga gctctcgccc tcgtcgaacc
     5161 tgcagaccgg cgcgatcgca ccgtggggcg acgagttcgc cgaccacccc ttcgacctgc
     5221 tgtaccagct gggcttctcg gtgacggtca acaccgacaa ccgcacgatg agcggcacgt
     5281 cgctcacccg cgagctggcg ctcctcgtcg acgcgttcga ctacgacctg gacgacctgg
     5341 aggagttcca gttcaacgcc gcttccgccg cgttcctgcc cgtcgaggag cgcgaggagc
     5401 tggtggagat gatcggcgag ggcttcgagc gctgagcgcg gcatgagctc cgtcttcatc
     5461 gcaggccccg cctcctggaa ccagctggtc gtgctggacc ggctgcccga gccggtgccg
     5521 cacatgcagt tcgccgagga ctcctgggag accctcggcg gcacgagtgc gggcaaggcg
     5581 ctgagcctca cggcgctggg gcgcgagacg ctgctgtacg ccgtcacggc ggacgatgag
     5641 ccggggcgtc gtgtgcgcgt ggcgctggag cgcgccggtg tcgcgacgcg ctgg
//
LOCUS       DATMSX010000320        15071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_99684,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000320 DATMSX010000000
VERSION     DATMSX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99684"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VN041_09815"
     CDS             complement(<1..1536)
                     /locus_tag="VN041_09815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWU29372.1"
                     /translation="MVTRLSNFFLRTLREDPADAEVTSHKLLVRAGYIRRAAPGVFTW
                     LPLGLKVKSRIERIVREEMGNAGAQEVHFPALLPRDPYEQSGRWESYGDGIFRLQDRK
                     GADYLLAPTHEEMFTLLVKDLYSSYKDLPLTIYQIQDKYRDEARPRAGLLRGREFTMK
                     DAYSFDASDEGLDASYQKQRDAYERIFQRLGLEYVIVNADNGLMGGARSEEFLHPTPV
                     GEDTFVRSVGGYAANVEAFTTAVPEPVDFSAVPAATIFDSPATPTIETLVAHANKTLD
                     GEYTAADTLKNVVLALTHLDGTRELVVVGIPGDREVDEKRAEVAFAPAEVETATPEDF
                     EKNPLLVRGYIGPWSPTGAVLGEESATGIRYLVDPRVSEGTSWITGANIDEKHAHSVV
                     AGRDFFADGIVEIANVREGDPAPDGSGPVELARGMEIGHVFQLGRFFAETLGLKVLDQ
                     NGKLVTVTMGSYGIGVTRILAIIAELNNDDKGLIWPASVAPFDVQVVAAGRDQVAFDV
                     AEDI"
     gene            complement(1595..2365)
                     /locus_tag="VN041_09820"
     CDS             complement(1595..2365)
                     /locus_tag="VN041_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWU29373.1"
                     /translation="MSTADKARTLTDLYSAPEILRVVNIWDVVSAKAIAALPETRALA
                     TAGHSIAASFGYSDGQIPRELMLDMVGRIAASVDLPVSADLDDGYGDAGETTRLAIGV
                     GIVGANVEDRLKPFAESVAQVEAIVKAGEAEGVSFALNARTDAIVRGGDKPLSDKLAD
                     AVERGRAYLDAGATAVFVPGILDADATRFLVDGIGAGRVSVIGLPGALTAAEYEALGV
                     ARISYGPLPQRVALTALQDTAADLYANGAIPAGTRALN"
     gene            2472..3893
                     /locus_tag="VN041_09825"
     CDS             2472..3893
                     /locus_tag="VN041_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWU29374.1"
                     /translation="MPQRRSHVAPSAEQSELAAALAALRAGIESPVEFSPEALAEAES
                     ATPDAPDLDLRDVPFVTLDPAGSKDLDQAMHLSRAGSGFQVRYAIADVPGFVKAGGAL
                     DAEARQRGQTLYLADGKIPLHPESLSEGRASLLADQERPALVWTFALDSAGAVTEFRL
                     ERALVRSRAQLDYVAAQKQLDAGQDGPLSLLGEIGGLRQQQEQQRGGASLNLPDEEVV
                     QKPDGTYSIERRSPLPIEDWNAQLSLMTGMAAAELMIGAKIGVLRTMPQPDEEAFARF
                     RRQTEALGRPWRKGRYGDYLRGLDKSDPMTLPVLEAAASLFRGAGYITFDGSVPTGDI
                     EQAAIGAPYAHATAPLRRLVDRWSLAICEAVSQGRPAPDWARESLPQLPDLMQQSAQR
                     ASTLDAETINRVEAALLHPLVGNSIDATVIELRGESRAAVQIAEPAVTASAPVRAGTA
                     PGDTVTLKLTSVDIAKGEVQFAA"
     gene            3890..4744
                     /locus_tag="VN041_09830"
     CDS             3890..4744
                     /locus_tag="VN041_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWU29375.1"
                     /translation="MSSLPGLDPAQQAWVAARMPDAQLVRDMSWGLVETTVLHVRAGG
                     DDVVVKAAGSSDTHLPREISAHAGYTAPLISTGHAARLRDASAEARVILLDHLPGELV
                     LGTDAEWQADTYVQAGVLLRRLHEQESRMDADYEARATAKQLYWLDQPHRIDPSIAAR
                     ARHALASAPAPAVMLVPTHGDWHPRNWLIDDGVVRAIDFGRFDFRPAATDFARMAAQQ
                     WRERPDLEAAFLDGYGSDPRDPELWRLEALRQAVGTACWAYKVRDEAFEQQGHRMLAE
                     ALAAYDGS"
     gene            complement(4835..5815)
                     /locus_tag="VN041_09835"
     CDS             complement(4835..5815)
                     /locus_tag="VN041_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU29376.1"
                     /translation="MKTRILGLGLEVSAIGLGCMGMSQSYGPNPGSREDMIAVLRGAV
                     DAGVTLFDTAEVYGPYVNEELVGEALEPVRDQVTIATKFGFDIGREGGLNSRPEHIRE
                     VADASLRRLRTDVIDLFYQHRVDPDVPIEDVAGTIAELVAAGKVRHFGLSEASAATIR
                     RAHAVFPVTAVQSEYSLWTRDPEAEVLPALEELGIGFVPFSPLVRGFLTGAIDANTTF
                     ADDDMRRGLPRFTEENRDANAALVAHVAELAAAKHATTGQVALAWLLAQRPWIVPIPG
                     TRRMSRIQENLAAADLELTPGELDDLDALAARIGVHGERYPESMQRTVNG"
     gene            5892..6608
                     /locus_tag="VN041_09840"
     CDS             5892..6608
                     /locus_tag="VN041_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HWU29377.1"
                     /translation="MLYPPEMRVLDAAREQVRALHGDPNHTVAAAAMDTTGRIHTAVN
                     DYHFTGGPCAELVVLGVAAATASGPLVTIVAVGDGDRDVIAPCGRCRQVLLDQHPDCD
                     VIIRDGDELRLVNVRNLLPHSYRHPDASPERFVRFSARYFEAVRDGVKTATTRLNDPC
                     APGPAWLLFEFDDEYRRLPGRVDSVVSKRFDEISDDDARLEGCEHADELRAGLREHYP
                     GVTDADTVEVVRFHTTGVTE"
     gene            6605..7930
                     /locus_tag="VN041_09845"
     CDS             6605..7930
                     /locus_tag="VN041_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HWU29378.1"
                     /translation="MTSFEHWLRASAHRLRTVDPDEQDDDDLEPLLDIIGDARVVAIG
                     ESMHRMHEFLQVRHRVFRFLARRAGFTTLVMESGVPEGALVDRWIRTGEGRLRDVLAE
                     GIGYRFGACQEMLDQVTWMREQHVRGSVPVRFAGMDIPDSSASVIPAVHAALALLDDA
                     DPHYAAHLRSTLLPLFSYLPADRTGLAQAAPAIQSYLALDAAVQQEITVALTDLVARM
                     RARRPDMIASLGGANAAEERVRFGIRAAESAQAGDAFLHAMVSAPSRTWPAANVRDVW
                     MADSVEWVLERAPRVLVAAANGHVQRTPFSAPPFVPEPMTTLGGHLADRLGADYVAIG
                     TAFGHGDAWLHRPRPDDAPGHSHPFVQSLGAPDPASADAAFSGAGIGDLLVDLRATPP
                     EAASALDRTVGLRNGDVLQPLPIREAFDAIAYIDEVTPWHTWIDENGLA"
     gene            complement(7942..8796)
                     /locus_tag="VN041_09850"
     CDS             complement(7942..8796)
                     /locus_tag="VN041_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29379.1"
                     /translation="MQSTRVTFPDGSLTGEGDVLIVDRERAVVVVDATPFHPVDHTWP
                     DQPGDSGTITVSDVVLDVSEAVMAAVSDEGELAVGTDIPVKRGAEGWTWLVGHRVDGE
                     IPASVVAGASARLEVDAARRAGLSRGHTACHLASLALDAALADLWRKEIGVDPLGHPD
                     FEGRANQTSFIHPDGSVDEYRLGKSLRKAGFDSEGFAASLAQREQRINDLLAGWIASG
                     GESRIDVEGPGISDRRTWRCVLPEGEVGFLCGGTHVHSVAEFASISVALDLSDPQLLV
                     MTATAVPA"
     gene            complement(8856..9188)
                     /locus_tag="VN041_09855"
     CDS             complement(8856..9188)
                     /locus_tag="VN041_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU29380.1"
                     /translation="MPAAELPAPAQAMVDAINAADTDAFVAAFAPDGFVDDWGTVKSG
                     PEGIRSWAESDAIGAGARMTVLSAETAGDTTSIRFGWRSSVFNGESAGVFVVRGDRLA
                     SFTIPPNH"
     gene            complement(9287..10438)
                     /gene="ispG"
                     /locus_tag="VN041_09860"
                     /gene_synonym="gcpE"
     CDS             complement(9287..10438)
                     /gene="ispG"
                     /locus_tag="VN041_09860"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWU29381.1"
                     /translation="MPAVNLGMPRIPEVLAPRRKSRQIRVGKVLVGGDAPVSVQSMTT
                     TKTTDINGTLQQIAELTASGCEIVRVAVPSQDDADVLHIIAAKSQIPVIADIHFQPKY
                     VFQAIDAGCAGVRVNPGNIRKFDDQVGAIAKAAQAAGVSLRIGVNAGSLDRRLLEKYG
                     KATPEALVESAVWEASLFEEHDFHDFKISVKHNDPVVMVKAYRQLAERGDWPLHLGVT
                     EAGPAFQGTIKSATAFGILLGEGIGDTIRVSLSAPPAEEVKVGHQILQSLNLRERKLE
                     IVSCPSCGRAQVDVYTLAENVTEGLKDMTVPLRVAVMGCVVNGPGEAREADLGVASGN
                     GKGQIFVKGEVIKTVPESEIVATLIEEANRIAAEMGPDAAVGTAQVITA"
     gene            10495..11121
                     /locus_tag="VN041_09865"
     CDS             10495..11121
                     /locus_tag="VN041_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HWU29382.1"
                     /translation="MSTILLTGFAPFDGAERNPSGDAVAAVAAGYDGPHRLIAATLPV
                     SFAGAAAHLHALIAEHDPDAVIATGLAGGSDRVAVERVGVNLMDARIPDNDGAQPIDV
                     PSEPGGPAARFATLPVKRIVSVLEEEGIPARASLSAGSYVCNHVLYTALGASRPGVPT
                     GFVHLPWSTATAPADAPSLSEQDLARAVRLVVDHAFDPDDSIPGGSIW"
     gene            11295..13172
                     /locus_tag="VN041_09870"
     CDS             11295..13172
                     /locus_tag="VN041_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWU29383.1"
                     /translation="MTDLNRRIRALAADPETSFVLIARDGVVELLTGEIVDVETLSDI
                     PLEGADGPREVFAMVPYRQVRERGFEAQDDGTPLRCLLVAEREGLDAEELRAALPDAP
                     IDLRGGGFDISDEEYAGIVEQVIAEEIGRGEGANFVIRRDYRAGYECDDRIAALTWFR
                     ALLEHERGAYWTFAVITPGQIAVGASPEAHVVARDGIVTMNPISGTFRHPAGGATRES
                     LTEFLSSTKETEELFMVVDEELKMMSQVCSDGGRITGPHLKEMSRLTHTEYMLRGRSR
                     LDPRDILRETMFAPTVTGSPMQNACAVIRRHERAPRGYYSGVAALFTPNADGGHDLDA
                     PILIRTAYLDRGHLRVPVGATLVRHSDPLGEVGETHGKAAGVLGAIGAIERDHVAETR
                     DDDAPAPARLADDPEIAALLASRNTRLADFWMQPQDAAATGRFGGRSALVVDAEDRFT
                     TMLAHQLRHLGLDVTIAPWSEVTDEAVDAADLVVSGPGPGDPRDPSSPRIARMQQIVA
                     RRRATGSPLLAVCLSHQILADGLDIELVPLGSPYQGVQKTVPVFGEEASVGFYNTFTA
                     RVSPGTTRIGDAEVAADAESGDVYALRGEHFASIQGHLESILSRDGIATLERLTAHAL
                     S"
     gene            complement(13198..14517)
                     /locus_tag="VN041_09875"
     CDS             complement(13198..14517)
                     /locus_tag="VN041_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWU29384.1"
                     /translation="MEILLYLGGILFMLVGLGVSIGLHEVGHLVPAKLFGVRVGQYMI
                     GFGPRLWSKRIGETEYGFKVLPVGGFISMSGMYPPSTKTGPAKGVFATLVQDARTAND
                     ETIAEGEEERVFYRLPVWKRVIVMLGGPVMNLVLAVLLFTLMASGIGIQQASTTVASV
                     SECMIPATAAQQQTCAADDPAAPAAEAGFEPGDRLVSMDGEPVATFAAASQIIQDSPG
                     RTLEVVVERKGEDVTLQLTPVAAQRQQTDAQGRPVTDKGGKPVTHSVGYAGITAQLEY
                     AHQPIWTGAQMTWQQVGGVTNLIIQLPQKLWNVGESLITGSQRDPNGPLSVVGVGRIA
                     GEVAATNAPVLDRLAVLLNLLAALNVALFVFNLIPLLPLDGGHIVVALWEGIKRLWAK
                     VTGRPEPAPVDATRLVPVTVVVAVLLIAMGALLIIADIIRPMNLLGG"
     gene            14588..>15071
                     /locus_tag="VN041_09880"
     CDS             14588..>15071
                     /locus_tag="VN041_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29385.1"
                     /translation="MTIRQLRLFRGAVASSVATILAAVSHTVGGGTAPHPLLILTVAV
                     LLSPLAALLVGTRIRLSGLTGAVLSTQFVFHALFELTGGIAPTGLVGGHQHGPIVLEL
                     APGTHPDLLSSSPGMLLAHLLAALVTTLLLWRGELLVRTIARWVRTLLRLPQTAPAHP
                     V"
BASE COUNT         2213 a         5394 c         5141 g         2323 t
ORIGIN      
        1 gatgtcctcc gccacgtcga aggcgacctg gtcgcgccct gcggcgacga cctgcacgtc
       61 gaacggggcg acggatgccg gccagatcag gcccttgtcg tcgttgttca gctcggcgat
      121 gatcgcgagg atgcgggtga cgccgatgcc gtaggagccc atcgtgaccg tcacgagctt
      181 gccgttctga tcgagcacct tcaggcccag ggtctcggcg aagaagcgac cgagctggaa
      241 gacgtgtccg atctccatgc cccgggcgag ctcgaccggg ccggagccgt caggggcggg
      301 gtcgccctcg cggacgttcg cgatctcgac gatgccgtcg gcgaagaagt cgcgccccgc
      361 gaccacggaa tgcgcatgct tctcgtcgat gttcgcaccg gtgatccacg acgtgccctc
      421 gctgacccgc gggtcgacca ggtagcggat gccggtggcg gactcctctc cgagcacggc
      481 accggtcggg gaccagggcc cgatgtatcc gcgcacgagg agggggttct tctcgaagtc
      541 ctcgggggtc gcggtctcga cctcggcggg tgcgaacgcg acctcggcgc gcttctcgtc
      601 gacctcgcgg tcaccgggga tgccgacgac cacgagctcg cgggtgccgt cgaggtgggt
      661 gagcgcgagc accacgttct tgagcgtgtc cgccgcggtg tactcgccgt ccagcgtctt
      721 gttggcatgc gccacgagtg tctcgatcgt cggcgtcgcg ggcgaatcga agatcgtcgc
      781 agcgggcacg gcggagaagt cgacgggctc ggggacagcg gtcgtgaacg cttccacgtt
      841 ggccgcgtag ccgccgacag agcgcacgaa ggtgtcctcg ccgaccggcg tcgggtgcag
      901 gaactcctcg ctgcgggcgc cgcccatcag accgttgtca gcgttcacga tcacgtactc
      961 gaggccgagg cgctggaaga tccgctcata ggcgtcgcgc tgcttctggt agctggcgtc
     1021 cagtccctcg tcggaggcgt cgaaggagta ggcatccttc atggtgaact cgcgaccgcg
     1081 cagcaggccc gcgcgcggac gcgcctcgtc gcggtacttg tcctggatct ggtagatcgt
     1141 cagcggaagg tccttgtacg aggagtacag gtccttgacc aggagcgtga acatctcctc
     1201 gtgcgtcggc gcgagcaggt agtcggcgcc cttgcggtcc tgcagacgga agatgccgtc
     1261 gccgtaggac tcccagcgtc ccgactgctc gtaggggtcg cgcggcagca gcgcggggaa
     1321 gtggacctcc tgcgcccctg cgttgcccat ctcctcgcgg acgatccgct cgatgcgcga
     1381 cttgaccttc agcccgagcg gcagccacgt gaacactcct ggcgccgcgc ggcggatgta
     1441 gccggcgcga acgagcagct tgtggctggt gacctctgcg tctgcagggt cttcgcggag
     1501 cgtgcggagg aagaagttcg atagacgtgt gaccacgggt cgattgtatc gaccgcgtgg
     1561 acgcccatcc gccgcacgtc ggttcggagc gccgtcagtt cagcgcgcgg gtgcccgccg
     1621 gaatcgcgcc gttcgcatag aggtcggctg cggtgtcctg cagcgcggtc agcgcgacgc
     1681 gctgcggcag tggtccgtac gagatgcgtg cgacgccgag agcctcgtac tcggccgcgg
     1741 tgagagcacc ggggaggccg atcacgctga ccctgcccgc gccgatcccg tcgacgagga
     1801 agcgggtggc atcagcgtcc aggatgccgg gcacgaagac ggcggtcgca ccggcgtcga
     1861 gataggcgcg gccgcgctcc acggcatccg ccagcttgtc cgagagcggc ttgtcaccac
     1921 cgcgcacgat ggcgtcggtg cgggcgttga gagcgaagga gacgccctcg gcttcaccgg
     1981 ccttgacgat cgcctcgacc tgcgcgacgg actccgcgaa cggcttcagg cggtcctcga
     2041 cgttcgcgcc gacgatgcca acgccgatcg cgaggcgggt ggtctccccc gcgtcaccgt
     2101 agccgtcgtc gaggtcggcc gagaccggca ggtcgacgga tgctgcgatg cggccgacca
     2161 tgtcgagcat gagctcccgg gggatctgcc catccgaata accgaagctc gcggcgatcg
     2221 agtgccccgc cgtggccagt gcgcgcgtct cggggagggc ggcgatcgcc ttcgccgaga
     2281 cgacgtccca gatgttgacg acacggagga tctcgggggc ggagtagagg tcggtgaggg
     2341 tgcgggcctt gtcagcggtg ctcatggctc cacgctagtc ccgctcgcgg acaccggcgc
     2401 gcggtcaggc cgacatgacg cgcggtcggt gctccccgtt tcccgaagcg tccgcggcat
     2461 aggctgacga catgcctcag cgtcgatcac atgtcgcccc gtccgccgag cagagcgagc
     2521 tggccgcagc cctcgccgcc ctgcgtgcgg gcatcgagtc gcccgtcgag ttctcgcccg
     2581 aggctctggc ggaggcggag tcggcgacac ctgatgcgcc ggatctcgat ctgcgcgacg
     2641 tgccgttcgt caccctcgac ccggcgggct cgaaagacct cgatcaggcc atgcacctgt
     2701 ctcgcgccgg ttcgggattc caggtgcgtt acgcgatcgc ggacgtcccc ggtttcgtga
     2761 aggccggtgg cgcgctggac gccgaggcgc gtcagcgggg acagaccctc tatctggccg
     2821 acgggaagat ccccctccac ccggagtcgc tctcggaggg aagggcctcc ctgctcgccg
     2881 atcaggagag gcccgccctg gtctggacgt tcgccctgga ctccgcggga gctgtgaccg
     2941 agttccgcct cgagcgcgcg ctggtgcgct cgcgcgcgca gctcgactac gtggcggcgc
     3001 agaagcagct ggatgcgggc caggacggcc cgctgtccct gctgggcgag atcggcgggc
     3061 tgcgtcagca gcaggagcag cagcgcggtg gcgcgagcct gaacctcccc gatgaggagg
     3121 tcgtgcagaa accggacggc acttacagca tcgagcgtcg gagtccgctg cccatcgagg
     3181 actggaacgc tcaactctcc ctgatgaccg ggatggccgc cgcagagctg atgatcggcg
     3241 cgaagatcgg cgtactgcgc accatgccac agccggatga ggaggcgttc gcacggttcc
     3301 gccggcagac cgaggcactc gggcggccgt ggcggaaagg ccgatacggc gactacctgc
     3361 gcggactgga caagagcgac ccgatgacgc tgccggtcct cgaagcagcc gcatcgctgt
     3421 tccgcggagc cggctacatc acgttcgacg gctccgtgcc gacgggcgac atcgagcagg
     3481 ccgcgatcgg ggcgccgtac gcgcacgcca ccgctcccct gcggcggctg gtcgaccgct
     3541 ggtcgctggc gatctgcgag gcggtgtcgc agggccgtcc cgccccggac tgggcgcgcg
     3601 agtcgctccc ccagctgccc gatctcatgc agcagtccgc ccagcgggca tcgacgctcg
     3661 acgccgagac catcaaccgt gtggaggccg cactgctgca tccgctggtc gggaacagca
     3721 tcgacgccac cgtgatcgag ctgcgaggcg agtcgcgcgc ggccgtgcag atcgccgagc
     3781 cggccgtgac ggcatccgcg cccgtgcggg cggggacggc tccgggcgac accgtgactt
     3841 tgaagctgac cagcgtggac atcgccaagg gcgaagtcca gttcgccgcg tgagttcgct
     3901 gccgggtctc gaccccgccc agcaggcgtg ggtggcggcg cggatgccgg atgcccagct
     3961 cgtgcgcgac atgtcgtggg gtctcgtcga gaccacggtg ctgcatgtgc gggcgggtgg
     4021 cgacgacgtc gtggtgaagg ccgccgggtc gtcggatacg catctgcctc gggagatctc
     4081 cgcccacgcg gggtacaccg cgccgctgat ctcgaccggt cacgccgcac ggctgcggga
     4141 tgcctcggct gaggcgcgcg tcatcctgct cgaccacctt cccggtgagc tggtgctggg
     4201 cacggacgcg gagtggcagg cggacaccta cgtgcaggcc ggagttctgc tcaggcgcct
     4261 gcacgagcag gagtcgcgga tggatgccga ttatgaggcg cgtgcgacgg cgaagcagct
     4321 gtactggctc gatcagccgc atcggatcga cccgtcgatc gccgcgcggg cgcgtcacgc
     4381 gctggcatcc gctcccgccc cggcggtgat gctggttccc acgcacggcg actggcatcc
     4441 gcgcaactgg ctcatcgacg acggcgtcgt gcgggcgatc gacttcggtc ggttcgattt
     4501 ccggcccgca gccaccgact tcgcgcgcat ggccgcccag cagtggcgcg agcgcccgga
     4561 cctggaggcg gcgttcctcg acgggtacgg ctctgatccc cgggatcccg agctgtggcg
     4621 gctggaagcc ctgcgccagg cggtcggcac cgcgtgctgg gcgtacaagg tgcgcgacga
     4681 ggcgttcgag cagcagggac accgcatgct ggccgaggct ctggctgcgt acgacggttc
     4741 atgacctcgg agatgtgcac gatctcggag gctttccgtg cgcagcatcc gagattctgc
     4801 gtatctccga agttctgccg cgcctcgacc cgactcagcc gttgaccgtg cgctgcatcg
     4861 actccgggta gcgctccccg tgcactccga tcctcgcggc gagcgcgtcg aggtcatcga
     4921 gctcccccgg agtcagctcc aggtcggccg cggcgaggtt ctcctggatg cgcgacatcc
     4981 tgcgggtgcc ggggatcggc acgatccagg gcctctgcgc gagcagccac gccagcgcga
     5041 cctgccccgt cgtggcatgc ttcgccgccg caagctcggc gacgtgggcc acgagtgccg
     5101 cgttcgcgtc gcggttctcc tccgtgaagc gaggcagacc gcgccgcatg tcgtcgtcgg
     5161 cgaaggtcgt gttcgcatcg atcgcgccgg tgaggaaacc gcggacgagc gggctgaacg
     5221 gcacgaagcc gatgcccagc tcctcgagcg ccggcagcac ctccgcctcc gggtctcgcg
     5281 tccacagcga gtactcgctc tgcaccgccg tcaccgggaa cacggcatgc gcgcgacgga
     5341 tcgtcgcggc ggaggcctca gacagaccga agtggcgcac cttgcctgcg gcgaccagct
     5401 cggcgatcgt tcccgcgaca tcctcgatcg gcacatccgg gtcgacgcga tgctgataga
     5461 acaggtcgat cacatccgtg cgcagccgcc gcagcgaagc gtccgcgacc tcgcggatgt
     5521 gctcgggccg gctgttcagc cctccctccc ggccgatgtc gaaaccgaac ttcgtcgcga
     5581 tcgtcacctg gtcgcggacc ggctcgagtg cctcgcccac caattcctcg ttgacgtacg
     5641 gcccgtagac ctcggccgtg tcgaacaggg tcacccccgc gtcgaccgcc ccgcgcagca
     5701 ccgcgatcat gtcctcccgc gatccggggt tcgggccgta gctctgcgac atgcccatgc
     5761 agcccagtcc gatggccgag acctccaggc ctagtccgag aatcctcgtc ttcatgtgtg
     5821 cactcctcat cgcctctgcg acgagcgtac gccgctgcca tgacgcagcg gaccgagtgc
     5881 gagactgaac catgctctac ccacccgaga tgcgcgtgct cgacgcggcg cgcgagcagg
     5941 tccgcgccct gcatggcgac ccgaaccaca cggtcgccgc agccgcgatg gacacgacgg
     6001 ggcgcatcca caccgccgtg aacgactacc acttcaccgg cgggccgtgc gcggagctgg
     6061 tcgtcctcgg tgtcgccgcc gccacagcat ccggcccact ggtcacgatc gtggccgtcg
     6121 gcgacggcga ccgcgacgtc atcgctccgt gcggacgatg tcgccaggtg ctgctcgatc
     6181 agcatccgga ctgcgacgtg atcatccggg acggcgacga gctgcgcctg gtgaacgtcc
     6241 gcaacctcct gccccattcc taccggcatc cggatgcctc accggagcgg ttcgtccgct
     6301 tcagcgcccg ttacttcgag gccgtgcggg acggggtcaa gaccgccacg acccgcctga
     6361 acgatccctg cgcgcccgga cccgcgtggt tgctcttcga gttcgacgac gagtaccgcc
     6421 ggcttcccgg acgcgtcgac tcggtcgtct ccaaacgatt cgacgagatc agcgatgacg
     6481 acgcccggtt ggaaggctgc gagcacgccg atgagctcag agccggtctg cgcgagcact
     6541 acccgggagt caccgatgcc gacaccgtcg aggtcgtgcg attccacacc accggggtga
     6601 ccgaatgacc tcgttcgaac actggctgcg cgcctcggcg catcgcctgc gcaccgtcga
     6661 ccccgatgag caggacgacg acgacctcga gccgctcctc gacatcatcg gcgacgcacg
     6721 tgtcgtcgcg atcggcgagt ccatgcaccg catgcacgag ttcctgcagg tacggcatcg
     6781 cgtcttccgc ttcctcgcgc gtcgtgcggg cttcaccacg ctggtcatgg agagcggggt
     6841 gcccgagggc gcactggtcg accgctggat ccgcacgggc gaggggcgac tccgcgacgt
     6901 gctggccgag ggcatcggct accgcttcgg tgcctgccag gagatgctcg accaggtcac
     6961 ctggatgcgc gagcagcacg tgcgcggttc cgtccccgtg cgcttcgccg gcatggacat
     7021 cccggactcc tcggcatcgg tcatccccgc tgtccatgcc gcgctcgctc tcctggacga
     7081 cgcggacccg cactacgccg cgcacctccg ctcgacgctg ctccccctgt tctcctacct
     7141 cccggcggac cgcacggggc tggctcaggc cgcgccggcc atccagtcct acctcgccct
     7201 cgatgccgcc gtgcagcagg agatcaccgt cgcgctgacc gaccttgtcg cgaggatgcg
     7261 cgcccgccgg ccggacatga tcgcgagcct cggtggagcg aacgcagccg aggagcgggt
     7321 gcggttcggc atccgcgccg ccgagagcgc ccaggcggga gacgcgttcc ttcacgcgat
     7381 ggtgtcggcg ccgtcccgca cctggcccgc cgccaacgta cgggacgtat ggatggcgga
     7441 ctccgtcgag tgggttctcg aacgcgcacc gcgcgtgctg gtcgccgcgg cgaacggtca
     7501 cgtgcagcgc acgccgttca gcgctccccc gttcgtcccc gagccgatga cgaccctcgg
     7561 cggccatctc gcggatcgcc tgggcgccga ctacgtggcg ataggcaccg ccttcggtca
     7621 cggcgacgcg tggctgcacc gacctcgtcc cgacgacgcc ccggggcact cccacccatt
     7681 cgtccagtcg ctcggagcgc ccgacccggc gagcgcggat gccgcgttct ccggcgcagg
     7741 catcggcgac cttctcgtcg acctccgcgc gactccgccg gaggccgcct ccgccctgga
     7801 tcgcaccgtg ggcctgcgca acggcgacgt gctgcagccc ttgccgatac gcgaggcctt
     7861 cgacgcgatc gcgtacatcg acgaggtcac gccgtggcac acctggatcg acgagaacgg
     7921 gctcgcctga ggctcagcgg ctcaggcggg gactgccgtc gccgtcatca ccagcagctg
     7981 cgggtcgctg agatcgaggg cgacgctgat ggacgcgaac tccgccaccg agtgcacatg
     8041 cgtgccgccg cagaggaagc cgacctcgcc ctcggggagc acgcagcgcc aggtgcgccg
     8101 gtccgagatg cccgggccct cgacatcgat gcggctctcg ccacccgatg cgatccagcc
     8161 ggccagcagg tcgttgatcc gctgctcgcg ctgggcgagc gaggccgcga agccctcgga
     8221 gtcgaacccg gccttgcgca ggctcttgcc gagccggtac tcgtcgacgc tgccgtccgg
     8281 gtggatgaag ctggtctggt tcgcccgccc ctcgaagtcg ggatgcccga gcgggtcgac
     8341 gccgatctcc ttgcgccaga ggtcggcgag cgcggcgtcc agcgccagcg aagcgagatg
     8401 gcacgcggtg tgaccgcggc tcagccccgc ccggcgtgcg gcatccactt ccagacgcgc
     8461 ggatgcccct gcgacgacgg atgccgggat ctctccgtcc acgcgatgac cgacgagcca
     8521 cgtccacccc tcggcgcctc gcttgaccgg gatgtcggtg ccgaccgcca gctcaccctc
     8581 atcgctgacc gccgccatca ccgcctcgga gacgtcgagc acgacgtcgg agacggtgat
     8641 cgtcccgctg tccccgggct gatcgggcca cgtgtggtcg accgggtgga acggcgtcgc
     8701 gtcgaccacg acgaccgccc gctcgcgatc gacgatcagc acatcgccct caccggtcag
     8761 agagccgtcg gggaacgtca cgcgcgtgga ctgcatgctc ccacgctacg cgacctcagg
     8821 cccgacgcac gacgtgacgg acgcccacgg agacctcagt ggttcggcgg gatcgtgaag
     8881 ctcgcaagcc tgtcgccgcg cacgacgaac acacccgccg actccccgtt gaagacgctg
     8941 ctgcgccagc cgaagcggat gctggtggtg tcgccggcgg tctcggccga gagcaccgtc
     9001 atccgggcgc ccgcgccgat cgcatcgctc tccgcccagg agcggatccc ctccggtccg
     9061 gacttcacgg ttccccagtc gtcgacgaag ccgtcgggcg cgaaggccgc gacgaatgcg
     9121 tcggtgtcgg cggcgttgat cgcatcgacc atggcctggg cgggggcggg cagctctgca
     9181 gcgggcatcg gcgtctcctc ggttcgagcc gggtcgagcc cggcatccgg acgccgagcg
     9241 taacccgcgc cggcctccgg gaccagcccc ggacccctga ctcccgtcag gcggtgatga
     9301 cctgggcggt gccgaccgcg gcatccggac ccatctcggc ggcgatgcgg ttcgcctcct
     9361 cgatcagggt cgcgacgatc tccgactcgg ggacggtctt gatgacctcg cccttgacga
     9421 agatctggcc cttgccgttg ccggaggcga caccgaggtc ggcctcacgg gcctcacccg
     9481 gtccgttcac gacgcagccc atgacggcga cgcgcagcgg cacggtcatg tccttcagac
     9541 cctcggtgac gttctcggcg agcgtgtaca cgtccacctg cgcacggccg caggacgggc
     9601 aggacacgat ctcgagcttg cgctcgcgca ggttcagcga ctgcaggatc tggtggccga
     9661 ccttgacctc ctcggcaggc ggtgcggaca gcgagacgcg gatggtgtcg ccgatccctt
     9721 cgccgagcag gatgccgaac gccgtggcgc tcttgatcgt gccctggaac gccggacccg
     9781 cctcggtcac gccgaggtgc agcggccagt cgccccgctc ggccagctgg cggtacgcct
     9841 tgaccatcac gacggggtcg ttgtgcttca cggagatctt gaagtcgtgg aagtcgtgct
     9901 cctcgaagag cgacgcctcc cagacggcgc tctccacgag ggcctccggc gtcgccttgc
     9961 cgtacttctc cagcagccgg cgatccagcg acccggcgtt gacgccgatg cgcagcgaca
    10021 cgcccgcggc ctgagcggcc ttcgcgatcg cgccgacctg atcgtcgaac ttgcggatgt
    10081 tgcccgggtt gactcggacg cccgcgcatc cggcgtcgat cgcctggaac acgtacttgg
    10141 gctggaagtg gatgtcggcg atcaccggga tctgactctt cgccgcgatg atgtgcagca
    10201 catcggcgtc gtcctgagag ggcaccgcga cacgcacgat ctcgcagccc gaggccgtga
    10261 gctcggcgat ctgctgaaga gtgccgttga tgtcggtggt cttcgtggtg gtcatcgact
    10321 gcacgctcac gggggcgtcg ccgccgacga gcaccttgcc tacccggatc tgacgggact
    10381 tgcggcgggg cgcgaggact tcggggatgc gcggcatccc aaggttcact gctggcactc
    10441 ctcaaggcta ccgtcgctga gtgcatgcgg gctggatgct cgtaggctga gcccatgtcg
    10501 acgatcctgc tgaccggctt cgcgcccttc gacggtgcgg agcgcaatcc ctcaggagac
    10561 gccgtcgccg ccgtcgccgc cggttacgac ggaccccacc gtctgatcgc tgccaccctg
    10621 ccggtctcgt tcgcgggcgc ggcagctcat ctgcacgccc tgatcgccga gcacgatccg
    10681 gatgccgtga tcgccacggg cctggcgggc ggcagcgacc gggtggcggt ggagcgcgtc
    10741 ggagtgaacc tcatggacgc gcgcatcccc gacaacgacg gcgctcagcc gatcgacgtg
    10801 cccagcgagc cgggcgggcc cgccgcgcgc ttcgccacgc tgccggtgaa gcgcatcgtc
    10861 agcgtgctcg aggaggaggg catcccggcg cgcgcgtcgc tgtcggcggg ctcgtacgtg
    10921 tgcaatcacg tgctctacac ggcgctcgga gcgtcccgtc ccggtgtacc gaccggattc
    10981 gtgcacctgc cctggtcgac cgcaacggct cccgccgacg cgccgagtct gtccgagcag
    11041 gacctggccc gggcggtgcg cctggtcgtc gaccatgcct tcgaccccga cgactcgatc
    11101 cccggcggca gcatctggtg acgtcacgcc gcaccggcgg ccatgtctga tgtgatactt
    11161 tcgcggtatg tccgcgaccc gcacctggtg gcccgccttc taggcggtgt ttcgcgaacc
    11221 acgagaacac gaccgccttc cggggcggtc ttttttcgtt ctccgcaccg gattcccaga
    11281 acctggagac cccgatgacc gacctgaacc gccgcatccg tgctctcgcc gccgaccccg
    11341 agacctcttt cgtgctgatc gcccgcgacg gcgtggtcga gctgctcacc ggggagatcg
    11401 tcgacgtgga gacgctctcc gacatcccgc tggagggtgc ggacggtccc cgcgaggtct
    11461 tcgcgatggt gccctaccgg caggtgcgcg agcgcgggtt cgaggcgcag gacgacggta
    11521 cccccctgcg gtgcctgctg gtcgccgagc gcgaggggct ggatgccgag gagctgcgcg
    11581 ctgcgctgcc cgacgcgccg atcgacctgc gcggcggcgg cttcgacatc tccgacgagg
    11641 agtacgcggg catcgtcgag caggtcatcg ccgaggagat cggccgcggt gagggcgcga
    11701 acttcgtcat ccgccgggac taccgcgccg gatacgaatg cgacgaccgc atcgccgcgc
    11761 tcacctggtt ccgtgcgctg ctcgagcacg agcgaggcgc gtactggacg ttcgccgtca
    11821 tcacccccgg acagatcgcg gtgggcgcga gccccgaggc gcacgtcgtc gcgcgcgacg
    11881 gcatcgtgac catgaacccc atctcgggga cgttccggca ccccgcagga ggcgcgaccc
    11941 gcgagtcgct gaccgagttc ctctcctcta ccaaggagac cgaggagctc ttcatggtcg
    12001 tcgacgagga gctcaagatg atgagccagg tctgctccga cggcggccgc atcaccggcc
    12061 cgcatctgaa ggagatgtcg cggctcacgc acaccgagta catgctgcgc ggacgcagcc
    12121 gcctggaccc gcgcgacatc ctccgcgaga cgatgttcgc cccgacggtg accggctccc
    12181 ccatgcagaa cgcctgcgcg gtcatccgcc ggcacgagcg cgcaccacgg gggtactact
    12241 ccggggtcgc cgcactgttc accccgaacg ccgacggcgg ccacgacctg gacgcgccga
    12301 tcctgatccg caccgcctac ctcgaccggg gacacctccg cgtacccgtc ggcgcgaccc
    12361 tcgtgcgtca ctccgacccg ctgggcgagg tgggcgagac gcacggcaag gctgccggcg
    12421 tgctgggcgc gatcggcgcc atcgagcgcg atcatgtcgc ggagacgcgc gacgacgacg
    12481 caccggcacc tgctcggctg gcggacgatc ccgagatcgc ggcgctcctg gcatcccgca
    12541 acacgcgcct ggccgacttc tggatgcagc cgcaggacgc agccgccacg ggccgattcg
    12601 gcggtcgcag tgctctggtc gtggacgccg aggaccgttt cacgacgatg ctcgcgcacc
    12661 agctgcgcca tctcggcctg gacgtgacca tcgcgccctg gtccgaggtg accgatgagg
    12721 ccgtcgacgc cgccgacctg gtggtgtccg gccccggccc cggcgacccg cgtgacccgt
    12781 cgtcgccgcg catcgcccgg atgcagcaga tcgtcgcccg gcgccgtgcg accggctccc
    12841 ccctgctggc ggtatgcctg agtcatcaga tcctcgccga cggtctcgat atcgaactgg
    12901 tgccgctggg ctctccatac cagggcgtgc agaagaccgt gccggtgttc ggggaggaag
    12961 cctcagtcgg cttctacaac acgttcaccg ctcgcgtctc ccccggaacc acccggatcg
    13021 gtgacgcgga ggtcgctgcg gatgcggaga gcggtgacgt ctacgcgctg cgcggcgagc
    13081 acttcgcctc gatccagggg catctggagt cgatcctgtc ccgtgacggc atcgcgacgc
    13141 tggagcggct gacggcgcac gcgctgagct gaccgcaggc gcgtcagccc gacagcttca
    13201 gcccccgagc aggttcatcg gcctgatgat gtcggcgatg atcagcaggg cgcccatcgc
    13261 gatcagcagc acggcgacca cgaccgtgac cggcaccagg cgcgtcgcgt ccacgggagc
    13321 gggttcgggg cgtccggtga ccttcgccca caggcgcttg atcccttccc acagtgcgac
    13381 cacgatatgc ccgccgtcca gcggcagcag cgggatgagg ttgaacacga agagcgcgac
    13441 gttcagcgcc gcgagcaggt tcagcagcac cgcgaggcgg tcgagcaccg gagcgttcgt
    13501 cgccgccacc tccccggcga tgcggccgac gccgaccacg ctgagcgggc cgttcgggtc
    13561 acgctgcgat ccggtgatca gcgactcgcc gacgttccag agcttctgcg gcaactggat
    13621 gatcaggttc gtcactccac cgacctgctg ccaggtcatc tgggcgccgg tccagatcgg
    13681 ctggtgggcg tactccagct gcgcggtgat gccggcgtag ccgacgctgt gggtgactgg
    13741 cttgccgccc ttgtcggtga cgggccggcc ctgggcatcc gtctgctgac gctgagctgc
    13801 gacgggggtc aactgcagcg tgacgtcctc gcccttccgc tcgaccacca cctcgagggt
    13861 gcgtccggga gaatcctgga tgatctgcga cgcggccgcg aacgtggcga cgggctcgcc
    13921 gtccatgctg accagacggt caccgggctc gaacccggcc tccgcggcgg gcgcggccgg
    13981 gtcgtctgcg gcacaggtct gctgctgcgc cgctgtcgca gggatcatgc actcactcac
    14041 cgaggcgacc gtcgtgctgg cctgctggat gccgataccg gatgccatga gcgtgaagag
    14101 cagcaccgcc aggaccaggt tcatgacggg cccgccgagc atgacgatca cgcgcttcca
    14161 caccggcagg cggtagaaga cgcgctcctc ctcgccctcg gcgatcgtct cgtcgttggc
    14221 cgtgcgggca tcctgaacca gggtggcgaa cacgcccttc gccggtccgg tcttcgtcga
    14281 gggcgggtac ataccggaca tcgagatgaa tccgccgacg gggagcacct tgaagccgta
    14341 ctcggtctcg ccgatccgct tcgaccacag ccgcggtccg aagccgatca tgtactggcc
    14401 cacgcgcacg ccgaacagct tcgcgggcac cagatggccc acctcgtgca gcccgatcga
    14461 cacgccgaga ccgacgagca tgaacaggat gccgcccaga tacagcagga tctccacccg
    14521 accacgctag tgccccgcac cgaggaatcc gctgtgccct cgctatgcat ccggctaggc
    14581 tgaagacgtg acgatccgcc agctgcgcct cttccgcggc gcagtggcct cttccgtcgc
    14641 gacgatcctc gccgccgtct cgcacaccgt cggtggcggc accgcaccgc acccgctgct
    14701 gatcctcacg gtggcggtgc tcctctcgcc gctggccgct ctcctggtgg gcacgcggat
    14761 ccgcctgtcc ggactcaccg gagccgtgct ctcgacgcag ttcgtcttcc atgcgctgtt
    14821 cgaactgacg ggcggcatcg cgcccacagg cctcgtgggc gggcaccagc acggcccgat
    14881 cgttctcgag ctcgcccctg ggacgcatcc cgacctgctc tcgtcgtccc ccggcatgct
    14941 gctcgcccac ctgctggcag ccctcgtcac cacgctgctg ctctggcgcg gcgaactgct
    15001 ggtccgcacg atcgcgcgct gggtgcgcac cctgctgcgc cttccgcaga ccgccccggc
    15061 gcatccggtg g
//
LOCUS       DATMSX010000321         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_52222,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000321 DATMSX010000000
VERSION     DATMSX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52222"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN041_09885"
     CDS             complement(<1..524)
                     /locus_tag="VN041_09885"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202494.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single-strand break in
                     duplex DNA and has been shown to have polymerase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU29386.1"
                     /translation="MAASGQVVQIDGRRLRVTNLDKVVYPETGTTKGEILAYVSRVAP
                     VMLPHVRGRPVTRKRWVDGVGTADAPAESFFTKQLEQGAPDWVRRMPIEHSDGPKEYP
                     LADDAATLVWFAQIAALELHVPQWRFSRTGERRNPDRFVLDLDPGEGVGLDGCAQVAR
                     LARPILSGMGLEAYP"
     gene            591..1463
                     /locus_tag="VN041_09890"
     CDS             591..1463
                     /locus_tag="VN041_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWU29387.1"
                     /translation="MRSIWKGAVTFGLVNVPVKVYSATEDHDISLHQVHAEDGGRIRY
                     QRVCEIDGETVPYSDIDRAYVDDDGQTVVLTKKDLDALPAEKSREIDVVEFVPTEQID
                     LLTLDRAYYLEPDSKSPKAYVLLRRTLEQTDRTAIVRFTLRQKTRLAALRVRGDVLVL
                     QTLLWADEVREAAFPSLDEDVKISDKELELSASLVDSYSSDFEPESFVDEYQKELRTL
                     IDAKIKAGETFDAAETFADEGEGDGGEVIDLMEALRASVERSKAERAGGKKDTEKKDS
                     EKKTAKSGGRKKAS"
     gene            complement(1497..2279)
                     /locus_tag="VN041_09895"
     CDS             complement(1497..2279)
                     /locus_tag="VN041_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWU29388.1"
                     /translation="MLHSLIQAPTALIPWLDPATIIGTAGPWALLVVCFIVFAETGLL
                     IGFLLPGDTLLVISGLLSHTSAALPHGVFGINVWVVALLIGLSAFIGGEVGYFIGHKG
                     GPAIFERKESGLFSRKNVERTNAFFERFGGITVILARFVPIVRTFAPVAAGVGHMPWK
                     RYSLYNFIGAVLWGFGLTMFGYVIGFIPPIATFVENYIDLILLAAVGGTAIVTLWHYL
                     SERRKVKKEAREAEASAPATTALRLDPEVFDRAPDLDGDGKH"
     gene            complement(2341..2422)
                     /locus_tag="VN041_09900"
     tRNA            complement(2341..2422)
                     /locus_tag="VN041_09900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Leu,seq:tag)
     gene            2532..3674
                     /locus_tag="VN041_09905"
     CDS             2532..3674
                     /locus_tag="VN041_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWU29389.1"
                     /translation="MSHPSLVVAERVRRRLRLEHTDPSSHPDEARHIAQTEVRRHNDL
                     ALQRGEAIIDDEAALIRDVLASVSGFGALQPLLDDPDIEEIWLNGPDRIFVARDGATE
                     RLDLRLTDAIVRDLVERMLQSTGRRVDISQPFVDASLPDGSRLHVAIADVVRGSWAVN
                     IRKFLPQHRTLESLVAQGSVPAHIAELLQGALREGRSIIVSGATHAGKTTLLGALLAS
                     VADSQRIITVEETFELAVEGPDVVALQGRQASLEGTGEITLRRLVKEALRMRPDRIVV
                     GEVRDAEALDLVLALNTGVPSAGTIHANSADEALEKLALLPLLAGRNIDRAFIAPALA
                     TSISYVVHCRREPDGSRRVVEVVAPTGEVVGGRILTTAVHRAERAV"
BASE COUNT          626 a         1204 c         1337 g          563 t
ORIGIN      
        1 gggtacgctt caaggcccat gccactcagg atcggacggg ccaggcgcgc gacctgcgcg
       61 catccgtcga ggccgacccc ctcgccgggg tcgagatcga gaacgaagcg gtccgggttg
      121 cgtcgctccc ctgtgcggga gaaccgccac tgcggcacgt gcagctcgag cgcggcgatc
      181 tgagcgaacc agaccagcgt cgcggcgtcg tcggcgagcg ggtactcctt ggggccgtcg
      241 gagtgctcga tgggcatccg cctgacccag tcgggcgcgc cctgctcgag ttgcttcgtg
      301 aagaagctct cggccggggc atccgccgtg cccacgccgt cgacccagcg cttccgcgtg
      361 accggccgcc cgcgcacgtg cggcagcatg accggcgcga cccgtgagac gtaggcgagg
      421 atctcgccct tggtggtgcc ggtctccgga tacacgacct tgtcgaggtt ggtgacgcgc
      481 aggcgtcggc cgtcgatctg cacgacctgc ccgctcgctg ccatggggac agattagccg
      541 ggcggcgcaa gcccctggcc ggaaccattg tgactggtga tacttgtgtg atgaggagca
      601 tctggaaggg cgcagtgacc ttcggcctgg tgaacgtgcc ggtgaaggtc tattcggcga
      661 ccgaggacca tgacatctcg ctgcaccagg tgcacgcgga ggacggcggt cgcatccgct
      721 accagcgcgt gtgcgagatc gacggcgaga ccgtgcccta ctcggacatc gaccgggcct
      781 acgtcgacga cgacgggcag acggtggtgc tcaccaagaa ggacctcgac gcactgccgg
      841 cggagaagag ccgcgagatc gacgtggtgg agttcgttcc caccgagcag atcgacctgc
      901 tgaccctcga tcgtgcctat tacctcgaac cggactccaa gtcgcccaag gcctatgtgc
      961 tgctgcgccg gaccctcgag cagaccgacc gcacggcgat cgtgcgattc acccttcgcc
     1021 agaagacccg tctggccgcc ctgcgggtgc ggggagacgt gctcgtgctg cagacgctgc
     1081 tgtgggcgga cgaggtgcgc gaggcggcgt tcccgtcgct ggacgaggac gtgaagatca
     1141 gcgacaagga gctcgagctg tcggcatccc tggtcgacag ctactccagc gacttcgagc
     1201 cggagtcgtt cgtggacgag taccagaagg agctgcgcac gctcatcgac gcgaagatca
     1261 aggccggcga gacgttcgac gcagccgaga ccttcgccga cgagggtgag ggtgacggcg
     1321 gcgaggtcat cgacctgatg gaggcgttgc gcgccagcgt cgagcgctcc aaggcggaac
     1381 gcgccggtgg gaagaaggac actgagaaga aggactccga gaagaagacc gcgaagtccg
     1441 gcgggcgcaa gaaggcgagc tgacgtcgtc gcgagcgatg ccggccggct cggggatcag
     1501 tgcttgccgt cgccgtccag gtcgggggcg cggtcgaaga cctcggggtc cagccgcagt
     1561 gcggtggtcg cgggagcgga tgcctcggcc tcccgcgctt ccttcttcac cttgcggcgc
     1621 tcgctgaggt agtgccacag cgtcacgatg gcggtgccgc cgaccgccgc gagcaggatc
     1681 aggtcgatgt agttctcgac gaacgtcgcg atgggcggga tgaagccgat cacgtagccg
     1741 aacatcgtca ggccgaagcc ccacagcact gcgccgatga agttgtacag cgagtaccgc
     1801 ttccagggca tgtgtccgac gccggcggcc accggagcga acgtgcgcac gatcgggacg
     1861 aagcgcgcca ggatgaccgt gatcccgccg aagcgctcga agaaggcatt cgtgcgctcg
     1921 acgttcttgc ggctgaagag gccggattcc ttgcgctcga agatcgccgg gccgcccttg
     1981 tgcccgatga agtagcccac ttcaccaccg atgaacgctg acaggccgat cagcagcgcc
     2041 accacccaca cgttgatccc gaacacgccg tgcggcaggg ccgcggaggt gtgtgacagc
     2101 agtcctgaga tcacgagcag ggtgtcgccc ggcagcagga atccgatcag caggccggtc
     2161 tcggcgaaca cgatgaagca cacgacgaga agcgcccacg gtccggcggt gccgatgatg
     2221 gtggccgggt cgagccaggg gatcagcgct gtcggcgcct ggatgagcga gtgaagcaag
     2281 agaggttccc gtcggtcgtg cagcggcgga tggaactggg ggatggaaca ggtcgatgcg
     2341 tgcgggagat gggacttgaa cccacacgcc caggggcaca ggaacctaaa tcctgcgtgt
     2401 ctaccgattc caccactccc gcgcgtcagc aagtctactg atggagggtt acgggttccc
     2461 gtacatctgc cgagccgagg ggctgtggat aactttgacg cgggattcgc ggattctgtc
     2521 atgatgcccg cgtgagtcat ccgtccctgg tcgtcgccga gcgagtccgg cggcgtctgc
     2581 gcctcgagca caccgacccc tcgtcgcatc cggacgaggc ccggcacatc gcgcagaccg
     2641 aggtgcggcg ccacaacgac ctggccctgc agcgcggcga ggcgatcatc gacgacgagg
     2701 cggcgctgat ccgggacgtg ctggcgtccg tctccggatt cggcgccctg cagccgctgc
     2761 tcgacgatcc ggacatcgag gagatctggc tgaacggccc cgaccgcatc ttcgtcgcgc
     2821 gggacggtgc gaccgagcgg ctggatctgc ggctgacgga tgccatcgtc cgcgacctgg
     2881 tggagcggat gctgcagtcc accggccgtc gagtggacat cagccagccc ttcgtggacg
     2941 cctccctgcc ggacggctcc cgcctgcacg tggcgatcgc ggatgtggtg cgcggctcct
     3001 gggcggtgaa catcaggaag ttcctgccgc agcaccgcac gcttgagtcg ctggtcgcgc
     3061 aggggtcggt gcccgcgcac atcgccgagc tgctccaggg agcgctgcgc gagggccgca
     3121 gcatcatcgt ctcgggcgcc acgcacgcgg gcaagaccac cctgctgggg gcactgctgg
     3181 catccgtcgc cgacagtcag cggatcatca cagtggagga gaccttcgag ctggccgtcg
     3241 aggggccgga cgtggtggcg ctgcagggcc ggcaggcgag cctggagggc accggcgaga
     3301 tcacgcttcg gcggctcgtc aaagaggcgc tgcgtatgcg ccccgaccgg atcgtggtgg
     3361 gcgaggtgcg cgacgcggag gcactcgacc tggtgctggc actgaacacc ggcgtcccgt
     3421 cggccgggac catccacgcc aactccgcgg acgaggcgct cgagaagctc gcgctgctgc
     3481 ctctgctcgc gggacgcaac atcgacaggg cgttcatcgc accggcactg gcgacctcga
     3541 tctcctacgt cgtgcactgc aggcgggagc ccgacggttc gcggcgcgtc gtcgaggtcg
     3601 tcgcgcccac aggcgaggtc gtgggcggtc gcatcctgac cactgcggtc catcgcgcgg
     3661 agcgggcggt atgacgctgc tgatcggggc gttgctcggg gcgggtctgc tgctctgcgc
     3721 gtcgccgtgg
//
LOCUS       DATMSX010000322         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_118819,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000322 DATMSX010000000
VERSION     DATMSX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118819"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VN041_09910"
     CDS             complement(<1..341)
                     /locus_tag="VN041_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29390.1"
                     /translation="MPADRTRTTPTPAQLRVWREYIETADALRRALDGRMLSESGMSS
                     GDYVVLLALSEAPRRTLRSSELADAVGWERSRLSHHLRRMERRGLITRDASEAEARGV
                     EVAIAAAGLEL"
     gene            433..1545
                     /locus_tag="VN041_09915"
     CDS             433..1545
                     /locus_tag="VN041_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU29391.1"
                     /translation="MGATIEDIEFGLNSFGDVATDAGRVMSDAESLRLLVDEAQLAES
                     VGLDVFSIGEHYKEDSVDSATPVVLAAAAQATSRIHLGTSVTVLSTQDPVRLYQQFAT
                     VDGISGGRTEMVLGRASAIESFPLFGYDIAEYEEIFEEKLELFMRLMRDDRVTWSGKY
                     RTPLDDVRLHPRMPQGGIPTWIGIGGSPDSVIRAARYGLPLMMAVIGGRPERFAGHVE
                     LYLRALAKYGHRELPVAQHSIGLIADSDDEAKNMHWQYWEPMVQQLSKERGFYPPTPE
                     RYAEEVDSGALYVGSPETVAQKMAKAFRDNHLSRFDLKYDLTHLPRAAREDSIRLFGE
                     RVAPRVRELLTEDPGSWYPTRRPVTQITKDGKAVHA"
     gene            1538..>2214
                     /locus_tag="VN041_09920"
     CDS             1538..>2214
                     /locus_tag="VN041_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWU29392.1"
                     /translation="MPDTTSPQEKSMTTIGILGAGKVGTALARLAVAAGHRALIAGSR
                     DPERIALTVEVLVPGATAVHAADAIRESDIVVLALPLGKHHSLDADLLRGKLVIDTMN
                     YWWETDGIRDDLNDPRTSTSELVQEHLVGARVVKALNHMGYHDLEDESRPAGAQGRKA
                     IALAGDDADDVATVSALVDALGFDPVLAGGLANGVVLQPGYPTFGANVTAEQLRELMD
                     AAAVQHGA"
BASE COUNT          354 a          802 c          739 g          319 t
ORIGIN      
        1 aacagctcca ggccggcggc ggcgatcgcc acctccacgc cgcgcgcctc cgcctcgctc
       61 gcgtccctcg tgatgaggcc gcggcgctcc atcctcctca ggtgatggga cagcctgctg
      121 cgctcccaac ccacggcatc cgccagctcc gaggagcgga gcgtgcgtcg cggcgcctcg
      181 ctcagggcga gcagcacgac gtagtcgccg gacgacatcc ccgactccga cagcatcctg
      241 ccgtccaggg ccctccgcag cgcgtcggcc gtctcgatgt actcgcgcca tacgcgcagc
      301 tgcgcagggg tgggcgtggt cctcgtcctg tccgcaggca tccgtcctcc tcgggaatgg
      361 atgggcccac cctacggttg acgcgattga cacgtcaacc aaatcgacgt catgcaggaa
      421 ggtcggcaca tcatgggcgc cacgatcgag gacatcgagt tcgggctgaa ctccttcgga
      481 gacgtcgcca ccgacgccgg ccgggtgatg tcggatgccg agtccctgcg actgctggtg
      541 gacgaggcgc agctggccga gtccgtgggc ctggacgtct tcagcatcgg cgagcactac
      601 aaggaggact cggtcgactc ggccacgcct gtggtgctgg ccgccgccgc gcaggcgaca
      661 tcccgcatcc acctgggcac ctccgtgaca gtgctgtcca cgcaggaccc ggtgcggctc
      721 tatcagcagt tcgccaccgt cgacggcatc tccggcggcc gcaccgagat ggtgctgggc
      781 cgggcatccg ccatcgagtc cttccccctg ttcggctacg acatcgccga gtacgaggag
      841 atcttcgagg agaagctcga gctgttcatg cgcctgatgc gcgacgaccg ggtgacctgg
      901 tcggggaagt accgcactcc gctggatgac gtgcgcctgc atccgcggat gccccagggc
      961 ggcatcccca cctggatcgg catcggcggc agccccgact ccgtgatccg cgccgcccgg
     1021 tacggcctgc ccctgatgat ggccgtcatc ggcgggcgcc ccgagcgctt cgccggccac
     1081 gtggagctgt acctgcgcgc cctcgccaag tacggccacc gggagctgcc cgtcgcacag
     1141 cactccatcg ggctgatcgc cgactcggac gacgaggcga agaacatgca ctggcagtac
     1201 tgggagccga tggtgcagca gctcagcaag gagcgcggct tctacccgcc gacgcccgag
     1261 cgctatgcgg aggaggtcga ctccggcgcg ctgtacgtcg gctcgcctga gacggtcgca
     1321 cagaagatgg cgaaggcgtt ccgcgacaac cacctgtccc gattcgacct gaagtacgac
     1381 ctgacgcacc tgccgcgcgc ggcacgcgag gacagcatcc gcctgttcgg cgagagggtg
     1441 gccccgcggg tccgcgaact cctcaccgaa gacccgggga gctggtatcc gacccgccgc
     1501 ccggtcaccc agatcaccaa ggacggcaag gctgtccatg cctgacacca cgagtcccca
     1561 ggagaagtcc atgaccacca tcggcatcct cggcgccggc aaggtcggca ccgcgctcgc
     1621 ccggctcgcc gtggcggccg gtcaccgcgc gctgatcgcc ggatcccgcg atcccgagcg
     1681 catcgcgctg accgtagagg tcctggtgcc gggcgcgacc gccgtgcacg ccgccgacgc
     1741 gatccgcgag tcggacatcg tggtgctcgc cctgccgctg ggcaagcacc actctcttga
     1801 cgcagacctg ctgcgcggca agctcgtgat cgacacgatg aactactggt gggagaccga
     1861 cggcatccgt gacgacctga acgatccccg cacctcgacc agcgagctcg tgcaggagca
     1921 cctcgtgggc gcgcgcgtgg tgaaggcgct gaaccacatg ggctaccacg acctcgagga
     1981 cgagtcccgt cctgccggcg cgcagggtcg caaggcgatc gcgctggccg gcgacgatgc
     2041 ggacgacgtg gcgacggtgt cggctctggt ggacgctctg ggcttcgatc cggtgctcgc
     2101 cggcgggctc gcgaacggag tcgtgctgca gcccggatac ccgaccttcg gcgcgaacgt
     2161 caccgccgag cagctgcgcg aactgatgga cgccgcggcc gtgcagcacg gcgc
//
LOCUS       DATMSX010000323        26803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_165931,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000323 DATMSX010000000
VERSION     DATMSX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 26803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26803
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165931"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2142)
                     /locus_tag="VN041_09925"
                     /pseudo
     CDS             complement(1..2142)
                     /locus_tag="VN041_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            complement(2395..2799)
                     /locus_tag="VN041_09930"
     CDS             complement(2395..2799)
                     /locus_tag="VN041_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HWU29393.1"
                     /translation="MSTVADKLAKKSTRRTGGKQVRLRLVYVDFWSAVKLSFLGAVAL
                     AIVTMVSFFLVFMVLQATGVLTQTSDFLDKITDGSIDLTSLVGLPQVMAFGAVVAILN
                     LIVFTVLGAVIAGIYNLAVKVTGGLLVGFMSN"
     gene            complement(2820..5411)
                     /gene="gyrA"
                     /locus_tag="VN041_09935"
     CDS             complement(2820..5411)
                     /gene="gyrA"
                     /locus_tag="VN041_09935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWU29394.1"
                     /translation="MTDEERPEPAHEHGRIDQVDLQSEMQRSYLDYAMAVIVGRALPD
                     VRDGLKPVHRRVIYGMYDGGFRPDKSFSKCARVVGEVMGQYHPHGDSAIYDTLVRLVQ
                     PWSLRYPLALGQGNFGSPGNMGAAAPRYTETKMAPLALEMVRDIEEETVDFSPNYDGQ
                     TQEPDVLPSRFPNLLVNGSIGIAVGMATNIPPHNLREVSAAALWALENPGLPREELLE
                     GLIQRIPGPDFPTGAQILGTKGIQEAYRTGRGSITMRAVVEVEEIQGRTCLVITELPY
                     QVNPDNLAVKIGELARDGKITGIADIRDESSDRTGQRLVVVLKRDAVAKVVLNNLYKH
                     TQLQDNFGANMLAIVDGVPRTLALDGFITNWLDHQLDVIVRRTGFRLRKAQARMHILQ
                     GYLKALDALDEVIALIRRSPTVDEAREGLKALLDIDDDQAQAILDMQLRRLAALERQK
                     IIDEATDLEAKITDYKDIIATEARQREIVRTELTEIVDRFGDERRTHILHGFDGDMSM
                     EDLIPVEEMVVTITREGYIKRTRSDNYRSQHRGGKGVKGAQLRADDLVEHFFVTTTHH
                     WLLFFTDKGRVYRTKTYEVPEAGRDAKGQHVANLLALQPEEKIAQVLAISDYQAKDYL
                     VLATRGGLVKKTRLGDYDTNRQGGVIAIRMREGDELVSAELVNADEDILLISAKGMSV
                     RFHATDDALRPMGRATEGVRGMKFKDDEDCLLSASIVHDDSYVFTVTDGGYAKRTTVG
                     EYKVQKRGGTGIKVAKLSDERGVLAGGLVVSEDDEVLVVLSSGKVVRSAVAEVPAKGR
                     DTMGVVFARMTGDDRILAVARNSERAIDDEDEADAETSEPQTPDAQNLETQSPTAESP
                     EAETPES"
     gene            complement(5584..7647)
                     /gene="gyrB"
                     /locus_tag="VN041_09940"
     CDS             complement(5584..7647)
                     /gene="gyrB"
                     /locus_tag="VN041_09940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWU29395.1"
                     /translation="MTPETPADDENAPSTGTEPNTQESKKVPGDYGADQIQILEGLEA
                     VRKRPGMYIGSTGPRGLHHLVYEIVDNSVDEALAGYADNIDVTILADGAVRVVDNGRG
                     IPVDPHSSDPTKSTVEVVLTILHAGGKFGGGAYAVSGGLHGVGSSVVNALSTRFEVAV
                     KQKGYVWRHVFADGGAPQQQLEKGEETSETGTSITFWPDPEIFVETIEFDYETLRTRF
                     QQMAFLNKGLRIALRDEREASAYEVDGPDGTSITKQPSDVFLYERGLVDYVEYLNKVR
                     KAEVVNEEIIDFEAEDTERKISLEVAMQWTTSYTENVFTYANTINTHEGGTHEEGFRA
                     ALTSLVNRYARANNLLKEKDDNLSGDDVREGLTAVISIKLGEPQFEGQTKTKLGNTEA
                     KAFVQKVVNDQLGDWFERNPAQTKNIIRKAIDAATARLAARKARETARRKSVFESAAM
                     PDKLKDCTSKDPSISEIFLVEGDSAGGSAVQGRDPHTQAILALRGKILNVERARLDKA
                     LGNKEVQAMIQAFGTGIGEDFDIDKARYHKIVLMADADVDGQHITTLLLTLLFRYMRG
                     LIEAGFVYLAMPPLYRLKWTNAPHEYVYTDVERDALLKHGSENGKRLPKDAGIQRYKG
                     LGEMNPKELWETTMDHTTRTLRQITIDDAAAADEIFSVLMGEDVESRRTFIQRNAKDV
                     RFLDI"
     gene            complement(7765..8250)
                     /locus_tag="VN041_09945"
     CDS             complement(7765..8250)
                     /locus_tag="VN041_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWU29396.1"
                     /translation="MMDPQPPETVATYLRLRGLKPSSKNWKRRKRIEVDDDNAPFSKG
                     RDPGTLGDILAKLTRDSGWEKTLAAEDLVRQWAELAGADTAKHSEPVSLERGVLTVKC
                     DSTAWAKNLQFMRAVIMTEIGNRYPDAGVENIRFIGPDVPSWKWGPRVVPGRGPRDTY
                     G"
     gene            complement(8228..9421)
                     /gene="recF"
                     /locus_tag="VN041_09950"
     CDS             complement(8228..9421)
                     /gene="recF"
                     /locus_tag="VN041_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWU29397.1"
                     /translation="MIVEHLNLGDFRNYATADLTLHRGPNVLVGSNGQGKTNLAEAIV
                     FLATLGSHRVSSDAPMVRDGKEFAVVRARLAHGERKVLVEVQLNRQGSNKARINGSPS
                     KTNELPRYAHVVLFAPEDLQIVRGDPSSRRRFADQLLIQRTPRMAGVIADYERVLRQR
                     TALLKSARARGVRGEGLSTLDVWDDKLISLGSEIIHARLRLASDLQGPLADAYTAIAG
                     ADHRPQLDWALSVRGGDPEEGEPDAELDVRGEIEEQFRAALQAKRSKEIERGLTLVGP
                     HRDDLILRVRDLPVKGYASHGESWSVALALRLASAELLRAESPAGDPVLILDDVFAEL
                     DADRRQRLAAITSGYEQVIVTAAVEGDIPEPLHRHVVRIHAGTISDERPESGEEVGDD
                     GPAAS"
     gene            complement(9492..9941)
                     /locus_tag="VN041_09955"
     CDS             complement(9492..9941)
                     /locus_tag="VN041_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29398.1"
                     /translation="MTLEPITPELARRIVERDERDSDHWHPEYPFADELVPLRMLSGQ
                     DAADAVFTLYMIREEDLAVGGFGFFGPPDESGTVEFGYGLIPSARGRGLATDAVTEGL
                     RIAAAAGARRAIADTDLDNAPSIRVLEKSGLVETHRSADKVFFARAL"
     gene            complement(9938..11080)
                     /gene="dnaN"
                     /locus_tag="VN041_09960"
     CDS             complement(9938..11080)
                     /gene="dnaN"
                     /locus_tag="VN041_09960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWU29399.1"
                     /translation="MRFQVNRDVFSEAVSFVVKLLPQRNPQPILAGVLIEASAEGLTL
                     SAFDYEASARTTIEATVDEPGTILVHGRLLSDIASRLPNAPIEIAVEEDGGIAVTCGS
                     ARFTLAAMPVEEYPSIPEVTGSTGVVPAEEFGTAIAQVAFAASRDDVTPVLTGVQLEV
                     SGTTLSLVATDRYRVSLRDVPWDGEGAEQTALVPARTLTEVGKTFAHAGNIEIAFSGA
                     GDREIIAFTAGNKTVTSLLIKGNFPPVRRLFPEQTDHYAVVNTGDLIEAVRRVALVLD
                     RSAPLRFTFGADGVTMDASGSEQARASESVDAHLNGGDEVTLGLNPQYLLEALGAVKS
                     EFTRVTFTSSDNANKLSPVLVTSQTSVDQAGLDSFKYLLQPNLLLR"
     gene            complement(11501..12892)
                     /gene="dnaA"
                     /locus_tag="VN041_09965"
     CDS             complement(11501..12892)
                     /gene="dnaA"
                     /locus_tag="VN041_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWU29400.1"
                     /translation="MTTAAHPDVPIWSTLRDRLAEDERVSPQLHGFLSLVVPAGVMGG
                     VLYLDVPNDLTAAQINKRLRIPLMEALSQIGDEVTSYRTVVNHELADQPTAPISVPDF
                     TTPEPVRSEPAAETPQALRHESRLNPKYTFDNFVIGQSNRFAHAAAVAVAEAPAKAYN
                     PLFIYGDSGLGKTHLLHAIGDYAQSLYAGVKVRYVSSEEFTNDFINSIANNRGAAFQS
                     RYREVDILMIDDIQFLQGRAETQEAFFHTFNQLHDHNKQVVITSDVAPKLLTGFEDRM
                     RSRFEWGLITDVQAPDLETRIAILRKKAQSESLHIPDEVLEYIATVVSSNIRELEGAL
                     IRVSAFASLNRSALDISLAQTVLRDIVDTTEDNVISPTDIITSTAAYFKLTVDDLYGS
                     SRSQQIAIARQIAMYLCRERTNLSLPKIGQLFGNRDHTTVMYACKKISELMKERRSVY
                     NQVTEITTQISRR"
     gene            13211..13348
                     /gene="rpmH"
                     /locus_tag="VN041_09970"
     CDS             13211..13348
                     /gene="rpmH"
                     /locus_tag="VN041_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWU29401.1"
                     /translation="MTKRTFQPNNRRRAKKHGFRARMRTRAGRAILSARRTKGRTELS
                     A"
     gene            13370..13720
                     /gene="rnpA"
                     /locus_tag="VN041_09975"
     CDS             13370..13720
                     /gene="rnpA"
                     /locus_tag="VN041_09975"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWU29402.1"
                     /translation="MLARPNRLTRGVDYRQVVRRGTRCGGPRLVTSVLPASDDRAPRF
                     GFIISKQVGTAVVRNTVRRRLKAVCAEFLDTVPEGTDVVIRALPASASADFADLRSDV
                     ARCLNRLVAGPVRT"
     gene            13717..14067
                     /gene="yidD"
                     /locus_tag="VN041_09980"
     CDS             13717..14067
                     /gene="yidD"
                     /locus_tag="VN041_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWU29403.1"
                     /translation="MSALPSYAFGTARFRASDLIRGILVLPRNAVLAFLVVYRAVISP
                     LYGDVCRYYPSCSAYAVGAVQQHGAVWGTILSAWRILRCNPWSKGGVDDVRPHAHFHY
                     ELTPHGFVVPARKD"
     gene            14071..15147
                     /gene="yidC"
                     /locus_tag="VN041_09985"
     CDS             14071..15147
                     /gene="yidC"
                     /locus_tag="VN041_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWU29404.1"
                     /translation="MGLDLLLASTPSPAAGNGGGFDLFSILLWPFKWIVETILVAWHW
                     LFTAFGMAPNAGLTWVLSIVGLVIVIRAAVFPLFVKQIKSQRKMMEIAPQLKKVQEKY
                     KGKRDQLSREAMSRETMALYKKHGTTPVSSCLPMLVQMPFLLGMFYTLRDVGDHAKHG
                     IGGVGLLHADLTKQFYDATLFGVAPLHIDLRTALETKPAGWEATVTILVVLVVLMIVS
                     QFYTQLQIISKNLSPEAKTGQAYQMQKIMLYVLPLGFIFSGVFFPLGVVLYWFVSNLW
                     TMGQQFLVIREMPTPGSDAARAREERLARRGKALNAEGKIVPIAVYEAEQQRLLEEAE
                     RAKEQAPKREQPMSKKRAKKKGSN"
     gene            15147..15641
                     /locus_tag="VN041_09990"
     CDS             15147..15641
                     /locus_tag="VN041_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HWU29405.1"
                     /translation="MTTDELKSSAESTIEELEQEGDIAADFLEELLDIADIDGDLNLD
                     VRQGRAYVSVEAEGDALSVLSAPETVQALQELTRLAVQSKTGSFSRMILDIGGSRDTR
                     RRQLEGLVNAAVAKLDDGASQASLPAMSSYERKLVHDIVSERGLVSESYGEGADRHTV
                     ISRG"
     gene            15742..16404
                     /gene="rsmG"
                     /locus_tag="VN041_09995"
     CDS             15742..16404
                     /gene="rsmG"
                     /locus_tag="VN041_09995"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWU29406.1"
                     /translation="MTESQLESEPAAAAEIFGDRIDLARRFTASLAAEGELRGLIGPL
                     ELPRLWTRHVLNSAIAAPLFHGSVADVGSGAGLPGLVLAIARPDVEWTLIEPMERRVT
                     WLNEQVESLELPNVRVMRARAEDVGLEFDVVTARAVSALRTLVPITAPLAKDGGELIF
                     LKGHNVDAEITAAQKVLKKHKVTDVSVEVLGEGVLTETTRVFRARVRRGESPVVDRVG
                     RA"
     gene            16618..16893
                     /locus_tag="VN041_10000"
     CDS             16618..16893
                     /locus_tag="VN041_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWU29407.1"
                     /translation="MTGYTCILRCADDTFYVGSAIDLDARLASHAQGLGSDYTTCRLP
                     VDLVWFAEFERIDEAFALEKRIQGWSHAKRQAFIEGGFVSSLRSSSH"
     gene            17100..17981
                     /locus_tag="VN041_10005"
     CDS             17100..17981
                     /locus_tag="VN041_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWU29408.1"
                     /translation="MDTPLARELADLSSRRKALREVNVTFSGETRIFTVSNQKGGVGK
                     TTSAVNLASALAELGAKVLVIDLDPQGNASTALSIPHTADTSSIYDVLINDLPFAEIV
                     QPSPESENLYCAPSTIHLAGAEIELVSQVAREFRLRRALKTYLDDNHMDVVIIDCPPS
                     LGLLTINAFTAATEVLIPIQAEYYALEGLSQLLGNIQMIQKHLNPELHLSTILLTMFD
                     ARTRLSQQVADEVRNHFPQQVLDAVIPRSVRVSEAPSFGQTVITYDGTSAGAVAYREA
                     AVEIAQRGERSSQNKEQ"
     gene            17981..18949
                     /locus_tag="VN041_10010"
     CDS             17981..18949
                     /locus_tag="VN041_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWU29409.1"
                     /translation="MAAKRTGLGRGIGALIPTADQAERPVDVFFPGAPKPKTEAEPAP
                     AAAEADLPEIPGIRLIQVDPNSIVPNPRQPRTHFDEDYLAELVHSVREFGVLQPVVVR
                     KNSDGDYELIMGERRTRAAREAGLTEIPAIVRETADEDLLRDALLENLHRSELNPLEE
                     ASAYQQLLEDFGITQEELATRIGRSRPQISNTIRLLRLPVPVQQRVAAGVLSAGHARA
                     ILSVEDPERMQRLADKVVNEDLSVRATEAAAKALPAAPGSKSKPQAGARRAYLDEVSA
                     KLGDRLNTRVQIALGARKGQVKIEFASIQDLNRILGELGEQEYGSR"
     gene            19147..20223
                     /locus_tag="VN041_10015"
     CDS             19147..20223
                     /locus_tag="VN041_10015"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HWU29410.1"
                     /translation="MTDNSRKTTRRAAGVAAIVTLAAATLLAAPAAQAAPKCPTPAKG
                     TPAINMCAQPGDLLDVRIGDVLPTQPSLGYDEVYYKLGRYTLGKDKINKKFDDWCEAN
                     GQGEAATADANSRLTDPGSFTCTIPVGQETAESIAPMKTVVIGPGGKLYLTDGHHTLT
                     SFFELADGGPNLHVRLRVLGNLTGLGQKQFWDTMQENGWTYLKDAEGGVITPKQLPTS
                     VGLENFEDDELRSLLYFGRDIGYASGSVPFQEFYWADWVRTSGAVDLAGWNRDERASY
                     LNTVERLTRAQTALAPDAVVANGLTASELGALTEWNDGKKPTGGEFGELSKPYEDAKP
                     GKLAYALEFKQLHGITSADTVGAR"
     gene            20293..20625
                     /locus_tag="VN041_10020"
     CDS             20293..20625
                     /locus_tag="VN041_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWU29411.1"
                     /translation="MTESIPRRASLEVLRAEASDELAVLIQERLRGGEDPWEFMEDLP
                     SIDELVVFLLRAENIKANDGVRPNSARHYRVLRQIALEYPELTKTVWGMLGERRHRRW
                     DPTVADAS"
     gene            20702..21262
                     /locus_tag="VN041_10025"
     CDS             20702..21262
                     /locus_tag="VN041_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HWU29412.1"
                     /translation="MLVEYDSDWPRLFEDAAEALRAVGNPAWTIEHIGSTAVPGMMAK
                     PVIDIAVRLVDQGDLDRHRHGLERGGWRVGSGLRTHRVMLFEQGGARLRIAHFFTPEQ
                     WPNAHQRVLRDWLRMHPDDAARYQDAKQEAARRATGRRSYNDAKTAVIQEIMDRARAA
                     RGLPAVNVHDKRAHASGHEEARHPEG"
     gene            complement(21305..21628)
                     /gene="trxA"
                     /locus_tag="VN041_10030"
     CDS             complement(21305..21628)
                     /gene="trxA"
                     /locus_tag="VN041_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWU29413.1"
                     /translation="MTAKATTEATWEQDVLQADGPVLVDFWAAWCGPCRMVAPVLDEI
                     QAEHPEKITILKLNVDENPNLAMQYQITSIPAMKVFQGGEVKTTIIGAKPKFALEQDL
                     AAFIG"
     gene            complement(21691..22647)
                     /gene="trxB"
                     /locus_tag="VN041_10035"
     CDS             complement(21691..22647)
                     /gene="trxB"
                     /locus_tag="VN041_10035"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWU29414.1"
                     /translation="MRQVIIIGSGPAGFSAAIYAARANLEPLLVASSVEVGGELTKTT
                     DVENFPGFPEGIQGPDLMVKLQEQAEKFGTEVLYDDVVSLELDGPVKKVTLGTGAVHE
                     ASAVIYATGSAYRKLGIEGEERLSGHGVSWCATCDGFFFREKTIAVVGGGDSAMEEAT
                     FLTRFASKVYVIHRKDTLRASKIMQERAFANDKIEFIWNSEVAEILGEFETTGVRLRS
                     TVDGTLSELDLQGVFVAIGNDPRTHLVHDKLDLTPEGTIWVDGRSSRTSVPGVFAAGD
                     VIDPTYRQAATAAGSGVVAALDVEHFLAGLEDASVPVEAIAG"
     gene            complement(22787..24409)
                     /gene="murJ"
                     /locus_tag="VN041_10040"
     CDS             complement(22787..24409)
                     /gene="murJ"
                     /locus_tag="VN041_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWU29415.1"
                     /translation="MSSLGRASAILGAGTLISRVTGLVRTIVLLAAIGSVGRASDAFS
                     TANQLPNNVFTIIQTGILTAVIIPQIVKSASHKDGGQAFISKLFTLGTVVFLAATGVA
                     MVLAPWLVFMYSSQFTPDQLALGTAFAYWCLPQIFFYGLYALIGETLNAKRVFGPYTW
                     APIANNVISIIGFIVFMMLFGDNRNHVEMWDPTMIAVMGGTATLGIAVQAALLLLAWR
                     RTGLHLRPDFHWKGVGLGEVGKLASWTFMMVIAGQLAGLAQSNIVSKASGDHPAGTIM
                     MAAWLVFMLPYSIFAVSIGTPYFTQIAEHTAEGRHDQVRGDVARSIRAISLFIVISAV
                     ALAVASVPATRIFTENGAFAEQAAPVLICYLVGLLPLSLLFIVQRTFYAYGDTRTPFW
                     FTIIQCSLIVVSALVAPLLVPLSQLAAAVALGQSLAGLVQTIIAVVLLRRRLGGLELP
                     RVALSLGRFVIAAIPAGFAGWGVYLLIGGADGWAVASTGMTGRLLGAVGTGVVGGVAF
                     VVYLVVLFVLRAPELSVVSGLVKRFLPARASR"
     gene            complement(24406..26553)
                     /locus_tag="VN041_10045"
     CDS             complement(24406..26553)
                     /locus_tag="VN041_10045"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HWU29416.1"
                     /translation="MTANSPRNGSRPRARRLLSRLAAVLVVAGIALGSSAPASAATDE
                     PKASADDVVALGISTGSGARVEPNGPLVSTITIANSTDDTLSAGSVDLEVNPTPLGDA
                     AALDSWLDDGSATGDFRTVATEPSPPVAAGDSIDVGSVADAAELGALAPGVYAVRARL
                     TGATTNDPETTAWNLTATSVLVIAPANTQSVGIVVPITATPDDDVLLSADELAKLTAP
                     DGMLSGQIDAVTDTPAILAVDPAIPAAIRMLGTSAPASALAWLQRLERLPNDKFALQL
                     GDADPATQAHAGQKSLLTAPDLTPLLQTADFPTSTPAPTAPSTATPTPALPDNAALMA
                     VMGSQPGILWPRGDLTSSDLATFDGYLGAPGTTIVPSTSFEGNPGAHAVVDGHSVLVT
                     DAAASARLSTAATSTDPIATDREIAAAAGHLFFTSRTSQTVLVGLDRSQKRSPVALRD
                     VLSAFASPAVRLATLRNSPPASATLRTTAGTVRAGALTAMLADEKRLQAFSSILDVPA
                     LMLAPERIRMLRSIAVGLSDAAFTTATTDRAKHVQDLLGAVSIQRPKPVQLIAASAPL
                     PVWVRNDLPWNVNVSLHSTPSDPRLDIQESTEVVAGRASSTRVDVPISARVASGEVKV
                     DFRLTSPTGVAIGPAETADVTLRADWEAIGLGILGGIIGLLFVFGLIRTVRRRRARAS
                     IDGTGSEEHPTDEHPADDTGPDEAPTSPTKENE"
BASE COUNT         4317 a         8776 c         9169 g         4541 t
ORIGIN      
        1 aaccgcatcc ccgcccgcgg caggccggaa caccccgcgc acgtcgacca cggggatgcc
       61 ggccagctcc gcacggcggg cgctgcgggt ggtcaccagt cgccgcagcc ggtcgatgta
      121 ctccggtcgc agcctctcga cgtcgtggat cgccgtgacc tcctcccacc gcagctggaa
      181 cacgtcgaca ggggcatgga gcacgccggc ctcctgcagc cggcgcccga tctcgagcag
      241 ggaccggcgc agcgcgggca gcgtcgccgt gaagaagaag tggctgtcct cgcggaaacc
      301 gacgcccgcc tgcgctgcgc gcacccagcg tcgagcggcg gcgcgcatcc accgcggccg
      361 cagcagcggg tgcgcgagca gccgggcgag cgcctccttc gatcgcgagc cgtggtcgtc
      421 ctccggcggg ctgtcgatga agccgctcac catgccgatg atcacctcgg gcgactcggt
      481 aagcgtcggt gggctcacca gcagcggcga cgccgtctcc ttgcgcccga agtcgtgccg
      541 gaacgcgtcc agcgctgcgg cgaagccagg gaagcgtccg gcatccagtg cggtcacgat
      601 ctcgtcgagg ctgctttcgg cgaagagtcg acgaagaacg gtatccgtcc gcaccatccc
      661 cgccatctcg accagtgccc gctccgacgc ggccgtgcgg gtcggagcgc cgccggtgag
      721 ctcaccgagc aggccgatcc ggccgagcag gaacgtgacc gcggcgagcc gcgccaccgc
      781 cagggctgtg ccgggcaggt agtcgatgcg caggtcgcgg ctgagatcca tcgccctgag
      841 cgcgcgctcc ggcacgtcca gcagtgcgcg ccacgacagt tctcgcaggg gcagggcgtc
      901 gagttcgtcg gcgatgctca gcagaccgtt cagccgaggg tcgtcctgcc agcggtccgg
      961 gtggaaccgt acggccttgc ggacgagcct gatcggggcc cgcagaatcc cagggcccgg
     1021 atgcgggttc ggtggcacga agcgggtgac caccccgtcc tcctcgcgca ggtagtcggt
     1081 gaaggccccg cgcagcccgt agtactcggt gatctgacgc atcatctcgg cggggccgcg
     1141 cccgagccag gtgctgacgt ccatcggata cggccgcacc ggcaggtact cgctgaagat
     1201 catgctctgc acgcgctgcc ggccgttcag cttcatcggc tcgggcggca gcgcggtcat
     1261 cgggcgggcc tgcagcacgt ggaccgtgcc gcctgcgacc gcccattcca tgtcctgcgg
     1321 gcgcccgaag tgcgcctcgg ccgcgcgggc gaggcgcgcg agtgtcttca gccggtcggg
     1381 cgagagcagg ggagcaccgg ttgctttcgc ctcctcctcg cgcaccccgc cgtcggcatc
     1441 ccggcggatg acgacctcgg cgcgacccgg ccggtactcg gcctgctcac cgtcgtcgtc
     1501 gagcaggtag tggtcgggcg tgacgaggcc ggacacgacc gcctctccga ggcctgccgc
     1561 gccgtcgacg acgatccggt cgcgcctccc gctgaccggg tcggcgctga acatcacgcc
     1621 ggcgagctcg gcatccacca gcacctgcac cacgaccgcg atgcgcacgg tgtgcgggtc
     1681 gacgtgacgc tcttgccggt acgacacggc gcggtcggtc cacagggagg cccagcagtc
     1741 gcggaccgca tcgatcacgg catccgcgcc gacgatgttc aggtaggtgt cctgctgccc
     1801 ggcgaaggcg gcgcccggca ggtcctcggc cgtggcgctc gagcgcaccg cgaccgggac
     1861 gtcgtcgccg agtgcccggt atgcggtcgc tatcccgtcg cgcaggtctt cgggcatggg
     1921 cgcggatgcc accacccgcc gcagctccgc cggctaggcg tcctcgacca gggcggcgag
     1981 gcccacggcg tcggctgccc gctcataggc gtccgtggtg acgacgaacc cgtccggcac
     2041 cggcagcccg gcccggatca gctccccgag gttcgccccc ttgcctccgg cgatctccag
     2101 gtcgtcccgg cccagctcgt ccagccccac caccgcgcgc atccgcactc cttagtgaac
     2161 gtctcgttca ctaaatcctc cccctgctcg ctcgtcgacg caacccttcg acgggctcag
     2221 ggatccacaa aggtgcagac agacgaggag ggggcggaga ggttcccgga gcgagcatcg
     2281 gggaggagcg aagcggaggg gtcccccgct ctgcgagcgc agggaacctc tccgccccct
     2341 ccccagacac gaaaaagccc ctcccgaatc cggaaggggc agtttcgttg tggatcagtt
     2401 cgacatgaac ccgacgagca ggccgcccgt caccttgacc gccaggttgt agataccggc
     2461 gatcaccgcg ccgagcaccg tgaagacgat caggttcagg atcgcgacga ccgcgccgaa
     2521 cgccatcacc tgcggcaggc cgaccaggct cgtcaggtcg atggagccgt cggtgatctt
     2581 gtccaggaag tcactggtct gggtgagcac gccggtcgcc tgcagcacca tgaacaccag
     2641 gaagaacgac accatcgtga cgatcgccag tgccacagca cccaggaacg acagcttcac
     2701 cgcagaccag aagtcgacgt agaccaggcg cagccgcacc tgcttgccac cggtgcgtcg
     2761 ggtggacttc ttcgccagct tgtctgcgac tgtgctcatc ggtcagctct cttcggtaat
     2821 caggattcgg gggtctcagc ctcaggggac tctgccgtag gggactgggt ttcgaggttc
     2881 tgggcgtcgg gggtctgggg ctcggaggtc tctgcatccg cctcgtcctc gtcgtcgatg
     2941 gcacgctcac tgttgcgtgc cacggcgagg atgcggtcat cgcccgtcat ccgggcgaac
     3001 acgactccca tggtgtcgcg gcccttggcg ggcacctcgg ccacggcaga gcgtaccacc
     3061 ttgccgctgg acagaaccac caagacctcg tcgtcctccg agacgaccag accgcctgcc
     3121 agaacaccgc gctcgtcgct cagcttggcc accttgatgc cggtcccgcc gcgcttctga
     3181 accttgtact cacccaccgt cgtgcgcttg gcgtagccgc cgtcggtcac ggtgaagacg
     3241 tacgagtcgt cgtgcacgat ggatgccgag agcaggcagt cctcgtcatc cttgaacttc
     3301 atgccgcgca cgccctcggt cgcacggccc atcggacgca gtgcgtcgtc ggtggcgtgg
     3361 aagcggaccg acatgccctt ggcgctgatg agaaggatgt cctcgtcggc gttcacgagc
     3421 tcggcgctga ccagctcgtc gccctcacgc atccggatcg cgatgacacc gccctgacgg
     3481 ttggtgtcgt agtcacccag gcgggtcttc ttcaccaggc cgccgcgggt ggccagcacc
     3541 aggtagtcct tcgcctggta gtcgctgatc gcgagcacct gggcgatctt ctcctcgggc
     3601 tgcagagcca gaaggttggc gacgtgctga cccttggcat cccggcccgc ctccggcacc
     3661 tcgtacgtct tggtgcggta cacccggccc ttgtccgtga agaacagcag ccagtggtgc
     3721 gtggtggtga cgaagaagtg ctcgaccagg tcgtcggcgc gcagctgcgc acccttcaca
     3781 cccttgccgc cacggtgctg cgaccggtag ttgtcgctgc gcgtgcgctt gatgtagccc
     3841 tcgcgggtga tggtgacgac catctcctcg accgggatca ggtcctccat cgacatgtcg
     3901 ccgtcgaagc cgtgcaggat gtgcgtgcgg cgctcgtcac cgaagcggtc gacgatctcg
     3961 gtgagctcgg tgcggacgat ctcgcgctgg cgagcctcgg tggcgatgat gtccttgtag
     4021 tcggtgatct tcgcctcgag atcggtcgcc tcgtcgatga tcttctgacg ctccagggcg
     4081 gccagacggc gcaactgcat gtcgaggatg gcctgggcct gatcgtcgtc gatgtccagc
     4141 agcgccttca gtccctcgcg cgcctcgtcg acggtggggg agcggcggat cagcgcgatg
     4201 acctcgtcga gggcatccag cgccttcagg taaccctgca ggatgtgcat gcgggcctgc
     4261 gccttgcgca gacggaagcc ggtgcgtcgc acgatcacat cgagctggtg gtccagccag
     4321 ttggtgatga agccgtccag cgcgagcgtg cgcggcaccc cgtcgacgat cgcgagcatg
     4381 ttcgcgccga agttgtcctg caactgggtg tgcttgtaca ggttgttcag gacgaccttg
     4441 gcgaccgcgt cgcgcttgag cacgaccacc agtcgctgcc cggtgcggtc ggaggactcg
     4501 tcgcggatgt cggcgatgcc cgtgatcttg ccgtcgcgcg ccagctcgcc gatcttcacc
     4561 gcgaggttgt cggggttgac ctggtacggc agctcggtga tgaccaggca ggtgcgcccc
     4621 tggatctcct cgacctcgac gaccgcgcgc atcgtgatcg aaccgcggcc ggtgcggtac
     4681 gcctcctgga tgcccttggt gccgaggatc tgcgcgccgg tggggaagtc cggaccgggg
     4741 atgcgctgga tcagaccctc gagcagctcc tcgcggggaa gacccgggtt ctccagagcc
     4801 cacagcgcag cggccgagac ctcacgcagg ttgtgcggcg ggatgttcgt ggccatgccg
     4861 accgcgatgc cgatcgaacc gttgaccagc aggttcggga accgcgacgg caggacgtcg
     4921 ggctcctggg tctgaccgtc gtagttcggc gagaagtcga cggtctcctc ttcgatgtcg
     4981 cgcaccatct cgagcgcgag cggagccatc ttggtctcgg tgtaccgggg tgcagcggca
     5041 cccatgttgc cgggggagcc gaagttgccc tggccgaggg cgagcgggta gcgcagcgac
     5101 cacggctgca ccaggcgcac gagggtgtcg tagatcgccg agtcgccatg cgggtggtac
     5161 tgacccatga cctcgccgac cacgcgggcg cacttcgaga acgacttgtc ggggcggaac
     5221 ccgccgtcgt acatgccgta gatgacgcgg cggtgcacgg gcttgaggcc gtcgcgcacg
     5281 tcgggcagcg cgcggccgac gatgacggcc atggcgtagt cgaggtagct gcgctgcatc
     5341 tcggactgca ggtcgacctg gtcgatgcgg ccgtgctcgt gggccggctc ggggcgttct
     5401 tcgtcagtca tgtcttttct tcttctccgg atgctgccgg actccggaat cttctcttca
     5461 ttcgcagaac gggttccctc ccgcttcgcg ggcccctccc gatgctcatt ccgagaagat
     5521 tcctccgtcc tacgtgcgtg ttctgttgtc cgagatcagc ggtgggtgag gagtaggggt
     5581 gtgtcagatg tcgaggaagc ggacgtcctt ggcgttgcgc tggatgaagg tgcgtcgcga
     5641 ctcgacgtcc tcacccatca gcacgctgaa gatctcgtcc gcagccgcgg catcgtcgat
     5701 ggtgatctgg cgaagggtgc gcgtggtgtg atccatcgtg gtctcccaca gctccttcgg
     5761 gttcatctca ccgagaccct tgtagcgctg gatgccggcg tccttgggca gacgcttgcc
     5821 gttctcgctg ccgtgcttga gcagggcatc ccgctcgacg tcggtgtaca cgtactcgtg
     5881 cggcgcgttg gtccacttca ggcggtacag cggcggcatc gcgaggtaca cgaaaccggc
     5941 ctcgatcagc ccgcgcatgt agcggaagag cagcgtcagc agcagcgtcg tgatgtgctg
     6001 gccgtcgaca tcggcatccg ccatcagcac gatcttgtgg taccgcgcct tgtcgatgtc
     6061 gaagtcctcg ccgatgcccg tgccgaaggc ctggatcatc gcctggacct ccttgttgcc
     6121 cagggccttg tccagccgag cgcgctcgac gttgaggatc ttgccgcgca gcgccaggat
     6181 cgcctgggtg tgcgggtcgc gaccctgcac cgccgaaccg ccggccgagt caccctccac
     6241 caggaagatc tcgctgatcg acgggtcctt gctggtgcag tccttgagct tgtcgggcat
     6301 cgctgccgac tcgaagacgc tcttgcggcg cgcggtctca cgcgccttgc gggcggcgag
     6361 gcgcgcagtg gccgcatcga tcgccttgcg gatgatgttc ttcgtctgcg cggggttgcg
     6421 ctcgaaccag tcgcccagct gatcgttcac gaccttctgc acgaacgcct tcgcctcggt
     6481 gttgccgagc ttggtcttgg tctgaccctc gaactgcggc tcgccgagct tgatcgagat
     6541 gaccgcggtc agaccctcgc gcacgtcgtc gccggagagg ttgtcgtcct tctccttgag
     6601 caggttgttc gccctggcgt agcggttgac caggctggtc agcgccgcgc ggaagccctc
     6661 ttcgtgcgtg ccgccctcgt gtgtgttgat cgtgttggcg taggtgaaca cgttctcggt
     6721 gtacgacgtg gtccactgca tcgcgacctc gagcgagatc ttgcgctcgg tgtcctcggc
     6781 ttcgaagtcg atgatctcct cgttgacgac ctcggccttg cggaccttgt tgaggtactc
     6841 gacgtagtcg acgagaccgc gctcgtagag gaacacgtcg gagggctgct tggtgatgct
     6901 cgtgccgtcg gggccgtcga cctcgtaggc ggatgcctcg cgctcgtcgc gcagggcgat
     6961 gcgcagtccc ttgttcagga acgccatctg ctggaagcgg gtgcgcaggg tctcgtaatc
     7021 gaactcgatg gtctcgacga agatctccgg gtccggccag aacgtgatgc tggttccggt
     7081 ctccgaggtc tcctcgccct tctccagctg ctgctggggg gcgcctccgt cggcgaagac
     7141 gtgccgccag acgtagccct tctgcttgac cgccacctcg aagcgggtcg agagcgcgtt
     7201 cacgaccgag gagcccacac cgtgcagacc acccgagacg gcgtatgccc caccgccgaa
     7261 cttgccgccg gcgtgcagga tcgtcagcac gacctcgacg gtcgacttgg tcgggtcgga
     7321 ggagtgcgga tcgaccggga tgccacggcc gttgtcgacg acgcgcaccg cgccgtcggc
     7381 gaggatcgtg acgtcgatgt tgtcggcgta gccggcgagg gcctcatcga ccgagttgtc
     7441 gacgatctcg tacaccaggt gatgcagacc gcgcggacct gtcgagccga tgtacatgcc
     7501 aggccgcttg cggaccgcct cgagaccctc gaggatctgg atctggtcgg cgccgtagtc
     7561 gccggggacc ttcttcgact cctgtgtgtt cggctccgtc cctgtcgagg gggcgttctc
     7621 gtcgtcggca ggtgtttcag gcgtcatcag aaatggttct ccacatcgaa agtacccctt
     7681 cattctagcg ggtttcgatg gtcgatatac gcctcaaacg ccgtgtgtgc gcctctgagc
     7741 gcttgagccc ccggtcgtga tgccctaccc gtaggtgtcc cgtgggcccc gtcccggcac
     7801 gacccgcggt ccccacttcc acgacgggac gtccgggccg atgaaccgga tgttctcgac
     7861 accggcatcc ggatagcggt tcccgatctc ggtcatgatc acggcgcgca tgaactgcag
     7921 gttcttcgcc cacgccgtgg agtcgcactt cacggtgaga acgccgcgct ccagcgacac
     7981 cggctcggag tgcttggcgg tgtcggcgcc ggcgagctcg gcccactgcc gcaccagatc
     8041 ctcggccgcg agcgtcttct cccagccgga gtccctggtg agcttggcga ggatgtcgcc
     8101 gagcgtgccc ggatcgcgcc ctttggagaa gggcgcgttg tcgtcgtcga cctcgatgcg
     8161 cttgcggcgc ttccagttct tcgaactggg cttcaggccg cgcagccgca ggtaggtcgc
     8221 gacggtctca ggaggctgcg ggtccatcat cgccgacctc ctctccggac tcggggcgct
     8281 cgtcgctgat ggttccggcg tggatgcgca cgacatgacg gtgcagcggc tccgggatgt
     8341 ccccttcgac ggcggccgtg acgatcacct gctcgtatcc ggaggtgatg gcggccaggc
     8401 gctgccgccg gtcggcatcc agctcggcga agacatcgtc caggatcagc accggatccc
     8461 cggcggggga ctcggcgcgc agcagttccg cggatgccag gcgcagcgcg agtgccacgg
     8521 accaggactc gccatgcgag gcgtacccct tcacgggaag atcgcggacg cgcaggatca
     8581 gatcgtcgcg gtggggcccg accagcgtca gcccgcgctc gatctccttc gagcgcttcg
     8641 cctggagcgc cgcgcggaac tgctcctcga tctcgccgcg gacatcgagc tcggcatcgg
     8701 gctcgccctc ctcgggatct ccaccgcgca cggacagcgc ccagtcgagc tgcgggcggt
     8761 ggtcggctcc cgcgatcgcc gtgtaggcgt cggcgagcgg accctgcaga tcggatgcca
     8821 gccgcaggcg cgcatggatg atctcggagc cgagcgagat gagcttgtca tcccacacgt
     8881 cgagcgttga gaggccctcg ccgcgcactc cccgcgcacg ggcggacttc agcagagcgg
     8941 tgcgctgccg cagcacgcgc tcgtagtcgg cgatcacccc tgccatccgc ggcgtgcgct
     9001 gaatgagcag ctgatctgcg aatcttctgc gcgacgacgg atcgccgcgg acgatctgca
     9061 ggtcctcggg cgcgaacagc acgacgtgcg cgtagcgcgg cagctcgttg gtcttcgacg
     9121 gagacccgtt gatgcgcgcc ttgttcgagc cctgcctgtt gagctgcacc tcgacgagca
     9181 ccttgcgctc accgtgcgcg aggcgtgcgc gcacgacggc gaactccttg ccgtcccgca
     9241 ccatcggggc atccgacgac acccgatgcg aaccgagcgt cgccaggaac acgatcgcct
     9301 cggcgaggtt ggtctttccc tgcccgttgc tgccgacgag cacgttaggg ccgcggtgca
     9361 gggtgagatc ggcggtcgcg taattgcgga aatcgcccag gttcaggtgc tccacaatca
     9421 cgaggatcag cctaccttcg gggtcggacg ctcagtccgg cgctgaatgc cggtgccgga
     9481 ggctctgcgt gtcagagtgc ccgcgcgaag aacaccttgt cggcggaacg gtgcgtctcc
     9541 acgaggccgg acttctcgag cacccggatc gagggcgcgt tgtcgaggtc ggtgtcggcg
     9601 atcgcgcgcc tcgcgccggc tgccgcagcg atccgcagcc cttccgtcac ggcatccgtg
     9661 gccagcccgc gaccgcgagc cgaggggatc aggccgtaac cgaactcgac ggtgccggac
     9721 tcgtccggtg gtccgaagaa gccgaaaccg cccacggcca ggtcttcttc tcgaatcatg
     9781 tacagcgtga agaccgcatc cgcggcatcc tgaccggaca gcatccgcag cggcaccagc
     9841 tcgtccgcga acggatattc cggatgccag tggtcgctgt cacgctcatc ccgctcgacg
     9901 atccgccgcg cgagctcagg tgtgatcggt tcgagcgtca ccgaagcagc aggttcggct
     9961 gcagcaggta cttgaacgaa tcgagacccg cctgatcgac cgaggtctgg ctggtcacga
    10021 gcaccgggct gagcttgttg gcgttgtcgc tcgacgtgaa cgtcacgcgg gtgaactcgc
    10081 tcttgaccgc gccgagcgcc tcgaggagat actgcgggtt caggccgagc gtcacctcgt
    10141 cgccgccgtt caggtgagcg tcgaccgact cggaggcgcg ggcctgctcg ctgccggagg
    10201 catccatcgt cacaccatcg gcgccgaacg tgaatcgcag cggcgccgag cggtcgagca
    10261 cgagagcgac acgacgcacg gcctcgatga ggtcgcccgt gttgacgacc gcgtagtggt
    10321 cggtctgctc ggggaacagc ctgcgcacgg gcgggaaatt gcccttgatc aggagagaag
    10381 tcaccgtctt gttgcccgcg gtgaacgcga tgatctcgcg gtcacccgct ccggagaacg
    10441 cgatctcgat gttgccggcg tgcgcgaagg tcttcccgac ctcggtcagc gtgcgggccg
    10501 ggaccagcgc agtctgctcg gcgccctccc catcccatgg cacatcgcgg agcgagacgc
    10561 ggtagcggtc ggtcgcgacc aggctcaggg tggtgccgga gacctcgagc tgcacaccgg
    10621 tgaggaccgg ggtcacatcg tcgcgggaag cggcgaacgc cacctgcgcg atcgcagtgc
    10681 cgaactcctc ggccggcacg acaccggtcg aacccgtgac ctcggggatg gagggatatt
    10741 cctcgaccgg catggccgcg agggtgaacc gggcggaacc gcaagtgacg gcgattccgc
    10801 cgtcctcctc cacggcgatc tcgatcggtg cgttcggaag ccggctggcg atgtccgaca
    10861 gcagacgacc gtgcacgagt atcgtgccgg gctcgtcgac ggtcgcctcg atcgtggtgc
    10921 gggcggatgc ctcgtagtcg aaggccgaga gggtcagccc ctcagcggat gcctcgatga
    10981 gcacacccgc caggatcggc tgcggattgc gctgtggcag cagcttgacg acgaaggaca
    11041 cagcctcgct gaacacatcg cggttgacct ggaacctcac gggcgctccc tcgttgtact
    11101 tcaccttttc gcagtgggcc cactgcaggc gatcccatgc tagtcgcacc gggcgaagcc
    11161 cccgctaccc cgaacagggc tgtgacggcc tgtccgtcca catccgtctg gtgatcgggg
    11221 agcccttcac tgagattgat ctcttaacgg tgttaacaac tgtggattct gtggataagt
    11281 cggtggatag cagcggagag taggaaacta cacgcttgtc agttgtggag accctgagga
    11341 ttccggggct tttggagggt tccgactccg aggcggcggt tcctgttatc cacaggtggg
    11401 tctgctcgac agcatccgga agccgaagtt tccacaagtt atccacaacc cctgcggaca
    11461 actgtcccga ttccgcggga cttcaggcgt gtcgggggcg tcagcgacgg ctgatctggg
    11521 tggtgatctc ggtgacctgg ttgtagaccg agcggcgctc cttcatgagc tcgctgatct
    11581 tcttgcaggc gtacatcacg gtggtgtggt cgcggttgcc gaacagctga ccgatcttcg
    11641 gcagcgacag gttcgtgcgc tcgcggcaca ggtacatggc gatctgccgg gcgatcgcga
    11701 tctgctggga acggctcgag ccgtacagat cgtcgacggt gagcttgaag tacgcggccg
    11761 tcgacgtgat gatgtccgtc ggcgagatga cgttgtcctc ggtggtgtcg acgatgtcgc
    11821 gcagcacggt ctgcgccagc gagatgtcga gcgccgagcg gttcaggctc gcgaacgcgg
    11881 agacccggat cagcgcgccc tccagctcgc ggatgttcga ggagaccacg gtcgcgatgt
    11941 attccagcac ctcatccggg atgtggagcg actcgctctg cgccttcttg cgcaggatcg
    12001 cgatgcgggt ctccaggtcg ggcgcctgga catccgtgat cagaccccac tcgaagcggc
    12061 tgcgcatccg gtcttcgaag ccggtgagca gcttcggagc cacatcgctg gtgatcacga
    12121 cctgcttgtt gtggtcgtgc agctggttga aggtgtggaa gaacgcctcc tgcgtctcgg
    12181 cgcgaccctg caggaactgg atgtcgtcga tcatcaggat gtcgacctcg cggtaccggc
    12241 tctggaaggc ggcgccgcgg ttgttcgcga tcgagttgat gaagtcgttc gtgaactcct
    12301 cgctcgagac gtagcgaacc ttcaccccgg cgtacagcga ctgggcgtag tcgccgatcg
    12361 cgtgcaggag atgggtcttg cccagtccgg agtcgccgta gatgaacagc gggttgtacg
    12421 ccttcgcggg cgcctcggcc acggcgaccg ccgccgcatg cgcgaagcgg ttggactggc
    12481 cgatgacgaa gttgtcgaag gtgtacttcg ggttgaggcg cgattcgtgc cgcagagcct
    12541 ggggcgtctc cgccgcgggc tcactgcgaa ccggttcggg ggtggtgaag tcggggaccg
    12601 agatgggggc cgtcggctgg tcggcgagct cgtggttcac caccgtgcga tatgaggtga
    12661 cctcgtcgcc gatctgggac agcgcctcca tcagcgggat gcgcagccgc ttgttgatct
    12721 gcgcggcggt cagatcgttg ggcacgtcca ggtacagcac cccgcccatc acgccggccg
    12781 ggaccaccag gctgaggaag ccgtgcagct gcggcgagac ccgctcatcc tcggcgagac
    12841 gatcgcgcag agtcgaccag atcggcacgt cggggtgggc tgcagtggtc atggtgctcc
    12901 gggcgaggtt gtggaaaggt cgctcgaagc gtggtctccc cctgtggata acgtcggatg
    12961 ccacggtagt catcacccgc cagaacatca agccgcccgc ggaaacacgc gggtgtgtcg
    13021 ctttctggcg cagtctccac aggtgtggat aatgggtgtg cgcaactgac gggcggcatc
    13081 gaggcatccg gcggtggatt tgccctgcgc ggcggcaggt cgtaccctta gtcggttgac
    13141 ttatgccttt tggcagtacc ccctgtctcc ggctgaaaaa gaccggtgac accagtcccg
    13201 gagtgattcc atgaccaagc gcaccttcca gcccaacaac cgccgccgcg ccaagaagca
    13261 cggcttccgt gcccgcatgc gcacccgcgc cgggcgtgcc atcctctcgg cacgccgcac
    13321 gaagggccgt actgagctct cggcgtaagc ccgctcaccc ggacctgccg tgctcgcccg
    13381 gccgaatcgc ctgacgcgtg gcgtcgacta ccgacaggtg gtccgccgcg gcacgcgatg
    13441 cggtgggcca cgtctggtga cttcggttct gccggcgtcc gacgaccgtg cacctcggtt
    13501 cgggttcatc atcagcaagc aggtaggaac cgctgtggtt cggaacaccg ttcgccgccg
    13561 cctgaaagcg gtgtgcgctg agttcctcga cacggtcccc gagggcacgg atgtcgtcat
    13621 ccgtgccctt cccgcatccg catccgccga cttcgccgac ctgcggagtg acgtggcacg
    13681 ctgtctcaat cggctcgtgg ccgggccggt gcggacatga gcgcgcttcc gtcctacgct
    13741 ttcggaacgg cgcggttccg ggcatccgat ctgatccgcg ggatcctcgt cctgccgagg
    13801 aacgcggttc tcgcgttcct cgtcgtctac cgggcggtga tctcgccgct gtacggggat
    13861 gtgtgcaggt attacccgtc ctgttcggct tacgctgtag gtgcggtgca gcagcacggc
    13921 gcggtgtggg ggacgatcct gtcggcctgg cgcatcctgc gctgcaatcc gtggagcaag
    13981 ggcggcgtcg acgacgtgcg tccgcacgct catttccact acgaactgac tccgcacgga
    14041 ttcgtcgtac ccgctcgaaa ggactgaaca gtgggtcttg accttctgct cgcaagcacc
    14101 cccagcccgg ccgccggcaa cgggggtggt ttcgatctgt tcagcatcct gctgtggccg
    14161 ttcaaatgga tcgtcgagac gatcctggtt gcctggcact ggctcttcac cgcattcggg
    14221 atggcaccga atgctggact gacctgggtg ttgtccatcg tcggtctcgt gatcgtgatt
    14281 cgcgcggcgg tcttcccgct gttcgtgaag cagatcaaga gccagcgcaa gatgatggag
    14341 atcgctccgc agctgaagaa ggtgcaggag aagtacaagg gcaagcgcga ccagctctct
    14401 cgtgaggcga tgagccgcga gacgatggcg ctgtacaaga agcacggcac cacgccggtg
    14461 tcgtcgtgtc ttccgatgct ggtgcagatg cccttcctgc tcggcatgtt ctacaccctg
    14521 cgcgatgtcg gtgatcacgc caagcacggc atcggcggcg tcggtctgct gcacgcggac
    14581 ctcaccaagc agttctacga cgcgaccctg ttcggtgtcg ccccgctgca catcgacctg
    14641 cgtacggcgc tcgagaccaa gcctgctggc tgggaggcca cggtcacgat cctcgtggtg
    14701 ctcgtggtcc tcatgatcgt gtcgcagttc tacacgcagc tgcagatcat ctcgaagaac
    14761 ctgtcacccg aggccaagac cggccaggcg taccagatgc agaagatcat gctctacgtg
    14821 ctgccgctgg gcttcatctt ctcgggcgtc ttcttcccgc tcggtgtggt gctctactgg
    14881 ttcgtctcca acctgtggac catgggtcag cagttcctgg tgatccgcga gatgccgacg
    14941 cccgggtctg acgccgcacg tgctcgcgag gagcgtctgg cccgtcgcgg caaggcgctg
    15001 aacgcggaag gcaagatcgt tccgatcgcg gtgtacgagg ctgagcagca gcgactgctc
    15061 gaggaggccg agcgggccaa ggagcaggct ccgaagcgag agcagcccat gagcaagaag
    15121 cgtgcgaaga agaaggggtc gaactgatga cgacggatga actgaagtcc tcggccgagt
    15181 cgacgatcga ggagctcgag caggaaggcg acatcgcggc cgacttcctc gaggagctgc
    15241 tcgacatcgc cgacatcgac ggcgacctga acctcgatgt gcgccagggc cgtgcctatg
    15301 tgtcggtcga ggcggagggc gatgccctgt ccgtgctgtc ggcgcccgag accgtgcagg
    15361 cccttcagga gctgacgcgc ctcgcggtgc aaagcaagac ggggtcgttc tcgcgcatga
    15421 tcctcgacat cggtggatcg cgcgacacgc gtcgccggca gctcgaagga ctcgtgaacg
    15481 ccgcggtcgc caagctcgac gacggggctt cgcaggcatc ccttcccgcg atgtcctcct
    15541 atgagcgcaa gctcgtgcat gacatcgtgt ccgagcgtgg actggtgtct gagtcgtacg
    15601 gcgagggcgc tgaccgccac acggtgatct cccgcggctg atcctcatcc gggatgtttc
    15661 acgtgaaaca tcccaggttg agcgatcgtt gagcgagcga agcgagtcga aattgcacat
    15721 cccctcgaag gagcccaccg cgtgaccgag tcgcagctgg aatcagaacc cgctgccgca
    15781 gcggagatct tcggtgatcg aatcgatctt gcgcgtcggt tcacggcatc cctggccgcc
    15841 gagggggagt tgcgcggtct catcggaccg ctggaactgc cccgactctg gacgcggcac
    15901 gtgctgaact cggcgatcgc tgcgccgctg ttccatggtt cggtggcgga tgtcggatcc
    15961 ggagccggcc tgccaggctt ggtgctcgcc atcgcgcgcc cggacgtgga gtggacactc
    16021 atcgagccga tggaacgtcg cgtcacctgg ctgaacgagc aggtggagtc gctggagttg
    16081 ccgaacgtgc gggtcatgcg cgctcgcgct gaagatgtcg gactcgagtt cgacgtggtg
    16141 acggcccgcg cggtgagcgc gttgcgcacg ctggttccga tcacggctcc gctggcgaag
    16201 gacggtggtg agctgatctt cctcaaggga cacaacgtgg atgctgagat cacggctgcg
    16261 cagaaggtgc tgaagaagca caaggtgacg gacgtctcgg ttgaggtgct cggcgaaggt
    16321 gtcctcaccg agacgacccg cgtcttccgc gctcgggtgc gccgagggga gtccccggtc
    16381 gtcgaccgag tgggacgagc gtgatgtttc acgtgaaacg ctgtaccggc cccaggtcgt
    16441 tgagcgagcg aagcgagacg aaacgtccat gtcctcgggc atttcgtctc ctcgcttcgc
    16501 tcgtcgctca atgaccggcg gagaggagtc ctccacagaa ggatgtttca cgtgaaacat
    16561 ccacagtcac cgagatcgaa atccgcaagc gtcgacgtcg ctcagtaccg tctcctcatg
    16621 actggttaca catgtatcct ccgctgtgct gacgacacgt tctacgtcgg cagcgcaatc
    16681 gacctcgacg ctcgactcgc atctcacgcg cagggtctgg ggagtgacta cacgacctgc
    16741 cgacttccgg tggatctggt ctggttcgca gaattcgagc ggatcgatga ggctttcgcc
    16801 ctggagaagc gcatccaggg ctggagccac gcgaagcggc aggctttcat cgagggcggc
    16861 ttcgtctcgt cgctgcgctc ctcgtctcac tgaccaggaa gaatcgtcgg ccgttgagcg
    16921 agcgaaacga cgcgaaacgc gcgtattgag gcccttcgtc cttgtcgcgg gagcgctcca
    16981 cactcaatga ccgggaaagg ggagtgggtg gtgtcgatta gagtggtaga cggtcccgaa
    17041 aggagtcgat gtttcacgtg aaaccatccg acaacgcatc gccgagccac gatttcggga
    17101 tggatacccc gcttgctcgg gaactcgctg atctgtccag ccgacgcaag gccctgcgtg
    17161 aggtcaatgt caccttctcg ggcgagaccc gcatcttcac ggtctccaac cagaagggcg
    17221 gcgtcggaaa gacgacgtcg gcggtgaacc tggcatccgc tctcgccgaa ctcggcgcga
    17281 aggtccttgt gatcgacctt gatccgcagg ggaacgcatc gacggctctc agtatcccgc
    17341 acaccgcgga cacctcgagc atctacgacg tgctgatcaa cgatcttccg ttcgcggaga
    17401 tcgtgcagcc gagcccggag tccgagaacc tctactgcgc gcccagcacg atccacctcg
    17461 ccggcgccga gatcgaactc gtctcgcagg tcgcacgaga gttccggctg cgccgcgctc
    17521 tgaagaccta tctcgatgac aaccacatgg atgtggtgat catcgactgc ccgccgtcgc
    17581 tgggcctgct cacgatcaac gccttcacgg ccgcgacgga ggttctgatc ccgatccagg
    17641 ccgagtacta cgcactggaa ggcctgagtc agctgctggg gaacatccag atgatccaga
    17701 agcacctcaa ccccgaactc cacctctcca cgatcctgct cacgatgttc gacgcgcgca
    17761 cgcgtctgtc gcagcaggtc gcggatgagg tccgcaatca cttcccgcag caggttcttg
    17821 acgccgtcat cccgcgttcg gtccgcgtgt ccgaggcccc gagcttcggc cagaccgtga
    17881 tcacgtacga cgggacgtcg gccggcgccg tcgcctaccg ggaagcagca gtcgagatcg
    17941 cacagcgagg cgaacggtcc tcgcagaaca aggagcagtg atggccgcca agcgcaccgg
    18001 actcggtcgg gggatcggag ccctcatccc caccgcggat caggccgagc gtcccgtgga
    18061 tgtgttcttc cccggagctc ccaagccgaa gacagaggcc gagccagcgc ccgctgctgc
    18121 cgaagctgat cttcctgaga tccccggtat ccggctcatc caggtcgacc cgaacagcat
    18181 cgtgcccaac ccgcggcagc cgcgtacgca cttcgacgag gattacctcg cggagctggt
    18241 gcacagcgtc cgggagttcg gtgtcctgca gcccgtcgtc gtccgcaaga actccgacgg
    18301 cgactacgag ctgatcatgg gggagcgccg tacccgcgca gcgcgcgagg ccggcctcac
    18361 tgagatcccc gcgatcgttc gcgagaccgc cgacgaggac ctgctgcgcg acgcgctgct
    18421 cgagaacctg caccgttcag agctgaatcc cttggaagag gcatccgcct accagcagct
    18481 gcttgaggat ttcggcatca cgcaggagga gctggctacg cggatcggcc gttcccgccc
    18541 gcagatcagc aacacgatcc gtctgctgcg cctgccggtt cctgtgcagc agcgcgtcgc
    18601 ggcgggtgtg ctgtcggccg gtcacgcgcg tgcgatcctc tcggtggagg accccgagcg
    18661 gatgcagcgc ctggccgaca aggtggtgaa cgaggacctg tccgtgcgcg ccacggaggc
    18721 ggctgcgaag gcacttcctg ccgcaccggg aagcaagtcg aagccgcagg cgggggcgcg
    18781 tcgcgcttac ctggacgagg tgtccgcgaa gctcggagac cggctgaaca cccgtgtcca
    18841 gatcgctctg ggagcgcgta agggccaggt caagatcgaa ttcgcgtcca ttcaggacct
    18901 caaccgcatt ctcggggagc tcggcgagca ggagtacggc tcgcgctgac cttctcgcac
    18961 agggcctggg atgtttcacg tgaaacatcc caggcccttt ctctttgaac agtcgtgagg
    19021 gcgcgcgcac ggcgtctcat cggtgcgtcc ttcactcgct ggcgccatcg ccgtttcacg
    19081 tgaaacatct cgtgtcgatc actccgcgtt cacagcgggt tcaccggcat ccgccactgt
    19141 gggcgcatga ccgacaactc ccgaaagacc acgcgccgcg ccgctggagt cgcagccatc
    19201 gtgacgctcg ctgcagcgac gctgctcgct gcacccgccg cgcaggcagc cccgaagtgc
    19261 ccgactcctg cgaaggggac tcccgcgatc aacatgtgcg cgcagcccgg tgacctgctc
    19321 gatgtccgca tcggcgacgt gctgcccacc cagccctccc tcggttacga cgaggtctac
    19381 tacaagctcg gccggtacac actgggcaag gacaagatca acaagaagtt cgacgactgg
    19441 tgcgaggcga acgggcaggg cgaggccgcg accgctgacg cgaactcgcg cctgactgac
    19501 cccggcagct ttacctgcac gatccccgtg ggtcaggaga ccgcggagag catcgcgccg
    19561 atgaagacag tggtcatcgg gcccggcggc aagctgtacc tcaccgatgg tcaccacact
    19621 ctgacgtcct tcttcgagct ggccgacggc ggcccgaacc tgcacgtgcg cctgcgcgtg
    19681 ctcggcaacc tcaccggtct cggccagaag cagttctggg acacgatgca ggagaacggc
    19741 tggacatacc tgaaggatgc cgagggcgga gtgatcacgc ccaagcagtt gccgacctcg
    19801 gtcggcctcg agaacttcga ggacgacgaa ctgcgcagcc tgctctactt cggtcgcgac
    19861 atcggctacg catccggttc cgtgcccttc caggagttct actgggcgga ctgggtgcgc
    19921 acctccggcg ccgtcgatct cgccggctgg aaccgcgacg aacgggccag ctacctgaac
    19981 actgtggagc gcctgacccg cgcgcagacc gccctggcgc cggatgccgt ggtcgcgaac
    20041 ggcctcacgg catccgaact cggtgccctg acggagtgga acgacggcaa gaagcccacg
    20101 ggcggtgagt tcggggagct ctccaagccc tatgaggacg ccaagcccgg caagctcgcg
    20161 tacgcgctcg agttcaagca gctgcacggc atcacgagcg ccgacaccgt cggcgctcgc
    20221 tgacacacgc ggaatccaca agagcgcggt cggatgccct ccggccgcgc tctcgtgcgt
    20281 aggctggaag tcatgaccga gtccatcccg cgccgcgcga gcctcgaggt gctgcgcgcc
    20341 gaagcgtcgg atgagctcgc ggtgctcatc caggagcggc tgcgcggggg agaggacccc
    20401 tgggagttca tggaggacct gcccagcatc gacgaactgg tcgtcttcct gctgcgcgcc
    20461 gagaacatca aggcgaacga cggcgtgcgg ccgaattccg cgcgtcacta ccgggtgctc
    20521 cggcagatcg cactcgaata cccggagctg acgaagacgg tgtgggggat gctgggggag
    20581 cggcgtcacc gccgctggga ccccacggtc gccgacgcct cctgacacgg gcgtacgggc
    20641 aaccgcggcg tccgccgcat ccccgaactg cgcgcggact acgctgtcgt cggaggcgca
    20701 gatgctggtg gaatacgact cggattggcc gcgtctgttc gaggacgcgg ctgaagcgct
    20761 gcgcgccgtc ggcaacccgg catggacgat cgagcacatc ggttccaccg ccgtgccggg
    20821 gatgatggcg aagcccgtca tcgacatcgc cgtccgtctc gtggatcagg gtgatctcga
    20881 ccgtcaccgg catggtctgg agcgcggcgg ctggcgtgtg ggcagcgggc tgcggacgca
    20941 ccgcgtgatg ctcttcgaac agggcggcgc acgcctcagg atcgctcact tcttcacgcc
    21001 ggagcagtgg ccgaacgcgc atcagcgtgt gctgcgtgac tggctgcgga tgcaccctga
    21061 tgacgccgcc cggtatcagg atgcgaaaca ggaggctgcc cgcagagcca cggggcggcg
    21121 cagctacaac gacgcgaaga cggcggtgat ccaggagatc atggaccgcg cgcgggccgc
    21181 ccgcggactg cccgcagtga acgtccacga caagcgggcg cacgcatccg gacacgaaga
    21241 agcccgccac cccgaagggt gacgggctcg cttcagcaga tgggctgctg ggcctgtcga
    21301 agcatcagcc gatgaacgcg gcgagatcct gctcgagggc gaacttcggc ttggcgccga
    21361 tgatcgtggt cttgacctcg ccgccctgga acaccttcat cgcggggatc gaggtgatct
    21421 ggtactgcat cgcgaggttg gggttctcgt cgacgttcag cttgaggatg gtgatcttct
    21481 cggggtgctc ggcctggatc tcgtcgagca ccggcgcgac catgcgacac ggaccgcacc
    21541 atgcggccca gaagtcgacg aggaccgggc catcggcctg gagcacgtcc tgctcccagg
    21601 tcgcctcggt ggttgccttg gcagtcatga ttctccttag ttggatgtca tccgatgcaa
    21661 caccggatgc gggaaaagtg ttccggatgc tcagccggcg atggcttcga ccgggacgga
    21721 ggcgtcttcg aggccggcga ggaagtgctc cacgtccagc gcggcgacga cacccgatcc
    21781 ggcggcggtc gcagcctgtc ggtaggtggg gtcgatcacg tcacccgcgg cgaacactcc
    21841 gggcaccgag gtgcgcgaag agcgaccgtc gacccagatg gtgccctccg gggtgagatc
    21901 gagcttgtcg tggacgaggt gggtgcgcgg gtcgttgccg atcgcgacga acacgccctg
    21961 caggtccagc tcgctgaggg tcccgtcgac cgtggagcgc aggcgcacac cggtcgtctc
    22021 gaactcaccg aggatctctg cgacctcgct gttccagatg aactcgatct tgtcgttcgc
    22081 gaatgcgcgc tcctgcatga tcttcgaggc gcgcagcgtg tccttgcggt ggatgacgta
    22141 caccttcgac gcgaagcgcg taaggaacgt ggcctcttcc atggcggagt cgccgcctcc
    22201 gacgacggcg atcgtcttct cgcggaagaa gaatccgtcg caggtggcgc accaggacac
    22261 accgtgaccg gagaggcgct cctcgccctc gatgccgagc ttgcggtagg cggatccggt
    22321 cgcgtagatg acggcggatg cctcgtgcac ggcgccggta cccagggtga ccttcttgac
    22381 tgggccgtcg agctcgagcg agacgacgtc gtcgtagagg acctcggtgc cgaacttctc
    22441 ggcctgctcc tgcagcttga ccatgaggtc ggggccctgg atgccctccg ggaagcccgg
    22501 gaagttctcg acgtcggtgg tcttggtcag ttcgccgccc acctcgacgg agctggcgac
    22561 cagcagcggc tccaggttgg cgcgcgccgc gtagatggcg gcgctgaacc cggccggacc
    22621 cgagccgatg atgatgacct gacgcatgtg ctctcctctg tcggcgggcg cgcggtgaca
    22681 cgcgggaaaa ccgccacgat ctcttcaacc catggtaact gcgcgacatt ccctgtcgct
    22741 gagcgcgact ggagcggcgg aggcgccgta gagaccagtg cggcgatcag cgcgacgcgc
    22801 gcgccggcag gaaccgcttg accagtccgg agaccacgct gagctccggg gcgcgcagca
    22861 cgaagagcac gacgaggtag accacgaacg ccacaccgcc gacgactccg gtgccgacgg
    22921 cgccgagcag acgtccggtc atccccgtcg aggcgacagc ccagccatct gcgccgccga
    22981 tcagcaggta gacgccccat ccggcgaagc cggcgggaat ggcggcgatc acgaagcggc
    23041 ccagcgagag cgcgacacgg ggaagctcca gaccgccgag acggcggcgc agcagcacca
    23101 cggcgatgat cgtctgcacg agccctgcca gggactggcc gagggcgacg gcggcggcga
    23161 gctggctgag cgggacgagc agcggagcca ccagcgccga gacgacgatc agcgagcact
    23221 ggatgatcgt gaaccagaac ggcgtgcggg tgtcgccgta ggcgtagaac gtgcgctgca
    23281 cgatgaacag cagtgagagc ggcagcaggc cgacgaggta gcagatcagc acgggcgcgg
    23341 cctgctcggc gaaggccccg ttctcggtga agattcgtgt cgcgggaacg gatgccacgg
    23401 ccagcgccac cgcggagatg acgatgaaca ggctgatcgc gcggatgctg cgggcgacgt
    23461 cgccgcgcac ctggtcatgt cggccctcgg ccgtgtgctc ggcgatctgc gtgaagtagg
    23521 gcgttccgat cgagaccgcg aagatcgagt aggggagcat gaacaccagc caggcggcca
    23581 tcatgatcgt ccctgccggg tggtcgccgg atgccttgga gacgatgttc gactgggcca
    23641 ggccggccag ctgaccggcg atgaccatca tgaacgtcca gctggcgagc ttgccgacct
    23701 cgccgagtcc gacgcccttc cagtggaagt cgggtcgcag gtgcagcccg gtgcggcgcc
    23761 aggcgagcag cagcagggcc gcctgcacgg cgatgccgag ggtggcggtg ccgcccatga
    23821 cggcgatcat cgtcggatcc cacatctcga cgtgattgcg gttgtcgccg aacagcatca
    23881 tgaagacgat gaagccgatg atggagatca cattgttcgc gatcggagcc caggtatacg
    23941 ggccgaacac gcgtttggcg ttgagcgtct caccgatgag cgcatagaga ccgtagaaga
    24001 agatctgcgg aaggcaccag taggcgaacg ccgtgccgag ggcgagctga tccggggtga
    24061 actggctcga gtacatgaac accagccacg gcgcgagcac catggccaca ccggtcgcgg
    24121 cgaggaagac gacggttccg agcgtgaaca gcttggagat gaacgcctgt ccgccgtcct
    24181 tgtggctggc ggacttcacg atctgcggga tgatgacggc ggtcaggatt cccgtctgga
    24241 tgatcgtgaa gacgttgttg ggcagctggt tcgcggtgct gaaggcgtcg ctcgcgcgcc
    24301 cgacggagcc gatcgccgcg agcagcacga tcgtgcgcac cagaccggtg acgcgcgaga
    24361 tcagcgtccc ggccccgagg atcgcgctgg ctcggccgag actgctcatt cgttctcctt
    24421 cgtcggcgag gtcggcgcct cgtccggacc ggtgtcgtcc gccggatgct cgtccgtcgg
    24481 atgctcttcc gagcccgtcc cgtcgatgct cgcgcgagcc cgacgtcgac gcacggtgcg
    24541 gatcaggccg aacacgaaca gcagtccgat aatgccgccg aggatgccga gcccgatcgc
    24601 ctcccagtcg gcgcgcagag tgacatcggc ggtctccgcc gggccgatcg cgacgccggt
    24661 ggggctggtg agccggaaat cgaccttcac ctcgccgctg gcgacgcggg ccgagatggg
    24721 cacgtccacg cgcgtgctgc tggcacggcc ggccacgacc tcggtgctct cctggatgtc
    24781 caggcgcgga tcggacggcg tgctgtgcag cgacacgttc acgttccagg gcagatcgtt
    24841 gcgcacccac acgggcagcg gtgcggacgc ggcgatcagc tgcacgggct tggggcgctg
    24901 gatgctcacc gcgcccagca gatcctgcac atgcttcgcg cgatccgtgg tcgccgtcgt
    24961 gaacgcggcg tcggagaggc cgactgcgat gctcctcagc atccggatcc gctcgggggc
    25021 cagcatcagc gccggaacgt cgaggatcga cgagaaagcc tgcagccgct tctcgtcggc
    25081 cagcatcgcg gtgagcgcgc cggcgcggac ggttcccgcc gtcgtccgga gagtggcgga
    25141 tgccgggggt gagttgcgca gcgtggcgag gcggacggcg ggagacgcga atgcgctcag
    25201 cacgtcgcgc agtgcgacgg gggagcgctt ctgcgagcgg tcgagaccca cgagcacggt
    25261 ctgcgaggtg cgggacgtga agaagaggtg cccggcggcg gcggcgatct cgcggtccgt
    25321 cgcgatggga tcggtgctcg tcgcggcggt ggacaggcga gcggaggccg cggcgtccgt
    25381 caccagcacg ctgtggccgt ccaccacggc gtgcgcgccc gggtttccct cgaacgaggt
    25441 ggatgggacg atcgtcgtcc ccggagcacc caggtacccg tcgaaggtcg cgaggtcgga
    25501 cgaggtgaga tcccctcgtg gccacaggat gccgggctgc gatcccatga cggccatcag
    25561 cgcggcgttg tcgggcagcg ccggggtcgg ggtcgccgtg ctcggcgcgg tcggggcggg
    25621 ggtgctggtc gggaagtccg cggtctgcag gaggggcgtg agatccggag cggtgaggag
    25681 ggacttctga cccgcgtgcg cctgagtggc agggtcggcg tcgccgagct gcagcgcgaa
    25741 tttgtcgttg ggaagccgct ccagccgctg cagccaggcg agcgcggagg ctggggcgct
    25801 ggttcccagc atccggatgg cggccgggat cgcggggtcg acggcgagga tcgcgggggt
    25861 gtcggtgacc gcgtcgatct ggccggacag cattccgtcc ggcgcggtca gcttggccag
    25921 ctcgtcggcg ctcagcagga cgtcgtcgtc gggagtggcg gtgatcggca cgacgatccc
    25981 gacgctctgc gtgttcgccg gggcgatgac gagcacggag gtcgcggtca gattccacgc
    26041 cgtggtctcg ggatcgttcg tggtcgctcc ggtgaggcgt gcgcgcaccg cgtacacacc
    26101 gggcgccaag gcgcccagct ccgcagcatc cgcgacgctt ccgacgtcga tgctgtcgcc
    26161 ggctgcgacg gggggtgacg gttcggtggc cacggtgcgg aagtcgcccg tggcgctgcc
    26221 gtcgtcgagc cacgagtcca gggcggcggc gtctccgagc ggagtcgggt tgacctcgag
    26281 gtcgaccgag ccggcgctca gcgtgtcgtc ggtgctgttc gcgatcgtga tcgtgctcac
    26341 caggggcccg ttcggctcca cccgggcccc ggagccggtg gagatgccca gcgcgaccac
    26401 gtcgtcggcg gatgccttgg gttcatcggt cgcggcggat gccggtgcgc tgctgcccag
    26461 tgcgatcccg gcgacgacga gcaccgcggc gagtcggctc agcagcctac gcgcgcgcgg
    26521 gcgggagccg ttgcgggggg agttcgcggt catggtgtcg ggtcgacggg gccgggagac
    26581 cggcgcaggc gacgccccga ttctagccag cgcgccgcgg tgaaccctga aggcgcgtag
    26641 agtgacggcc gtgccggatc gtgcccaccc ccgcctcgac ctcgttctcg cgcaggcgct
    26701 cgccgccgat ctggacgccg ccgacttccg ctccgaacct ctgcggcggc tgtggggtga
    26761 ggaggcggat gacgcgctgg cgcgcggact gcgcgagccg atc
//
LOCUS       DATMSX010000324        11539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_62174,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000324 DATMSX010000000
VERSION     DATMSX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62174"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VN041_10050"
     CDS             complement(<1..495)
                     /locus_tag="VN041_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWU29417.1"
                     /translation="MAYAVIDLETTGFSPARGDRIIELAVVLVDEAGRPEREWTTLVN
                     PQRDAGRAQVRGIRSEDLAGAPLIGEVASHLVGLLSGRTLVAHNQEFDLRFLKSELAS
                     DGFVLPATFDALDTMLWAHRAFGVSRLGDVCAVLDIDYDPARPALGDARAKAAILAAL
                     IAREG"
     gene            complement(609..1532)
                     /locus_tag="VN041_10055"
     CDS             complement(609..1532)
                     /locus_tag="VN041_10055"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HWU29418.1"
                     /translation="MTDEMPNWEGFMIPTLRVLSDGVVRHWREFQPLVADLVKLTDEQ
                     KTELLPSGSQLKYENRIGWGVSFLTNVGALDRPKRGNYTITDAGRQLIELFPDGVKER
                     DIRELGEDPSSPIRVYEKTERAKASTPTTGDVAESMTPIEQVQQGIERIHDEVASELI
                     SRLQGKEPGFFEQAVVDLLLAMGYGGTNGTGTVTQLSNDGGIDGVIDQDVLGLSRVYV
                     QAKRYASDNVVQRPDIQGFVGALAGKADSGVFITTSRFSEGARNYAEAVPTRIILIDG
                     SRLARLMIRYGVGVQTYETFKAIEIDEDFFA"
     gene            complement(1710..2549)
                     /locus_tag="VN041_10060"
     CDS             complement(1710..2549)
                     /locus_tag="VN041_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29419.1"
                     /translation="MPAAVPIQRITRPAVIVGSADDEALGGEHPWVWATGLPYHALAA
                     PSSGAVMPQSPRALLQRNEYGHVEGDFDHAIGYFAALQSFLTYSLGWTRHDKGLIWWL
                     DQGAPAADARFGLIRDVWFGDETLENYLSWCVEHPHIGVFDAFEHVVDDQPLELSEDW
                     RARLSTIRAQRDVDDLSLRPDQMHLQGGEHIGRPATGHADARLIGVDPGSRSAVLISQ
                     TVSGWSAALMRLGSELPTLSGGRSWRVDVHVKPVGFLGTFRRSRATGLWFAGRHAHHI
                     VGN"
     gene            complement(2549..5083)
                     /locus_tag="VN041_10065"
     CDS             complement(2549..5083)
                     /locus_tag="VN041_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWU29420.1"
                     /translation="MTRPESELLPAKPEAHLRIYAYSIADDAHSGWLKVGQTTQGVKT
                     RVAQQTKTAAITNYTIELDESAERHDGTLFTDHDVRARLKEKGFDNPTLEWMACAIDD
                     VRAAIAELRDGLRYTGTHHATFEMRAEQQAAVDKTYDYFQSIWADNADDAPEFLWNAK
                     MRFGKTFTTYQLAKKMGAKRVLVMTFKPAVEDAWDTDLKTHVDFDGWQYLSRKIGGDP
                     RDIDPEHPLVFFGSFQDLMGRDKAGNFKAKHDWLRDLHWDLVVFDEYHFGAWRDTAKE
                     LFDGEDDATRKKATAQEFGADLETFAEELEENGFDEDAFVPIRAGAYLYLSGTPFKVL
                     KDGRFIEEQIFNWTYTDEQRAKAEFASQHPDQKNPYASLPEMRLLTYQMPDELISIAS
                     QGEFDEFDLNAFFEAKGTGADAAFVHKDDVQKWLDIIRGSYAPTQLDALKAGTKPPFP
                     YSDTRLLPYLQHSIWMLPRTASCAAMANLLAEKHNVFWHDYRVVNVSAPGVGVGLDAL
                     PPVRKAIGAGFDTKTITLTVGKLTTGVTVPPWSSILMLRNLQAPESYFQAAFRVQSPW
                     TIRNPEGDDPNAEEILKPVCFVFDFAPTRALRQVSEYGTGLAPEEKNPERAVEELVKF
                     LPVLAYDGSNMTQVDAGGILDIAMSGTSATLLARKWESALLVNVDNLTLKKIMANEKA
                     MEAVMNIEGFRALGSDVFETVVNKSEAVSKTKKEKGDDLDTAEKKQLSEEEKEYKSKR
                     KQIQEKLIKFATRIPAFMYLTDYREQTLTDVITKIEPGLFQAVTGLTVDDFNTLVGLG
                     VFNASHMNQAVFAFRRYEDASLSYTGIESHEGLRRYGLYDTVVAVE"
     gene            complement(5070..6734)
                     /locus_tag="VN041_10070"
     CDS             complement(5070..6734)
                     /locus_tag="VN041_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HWU29421.1"
                     /translation="MSAKASFALQSHNPDVLTCIANLSNDEVFTPPELANQMLDTLER
                     SWAESNGGASIWADSTVTFLDPFTKSGVFLREITRRLTDGLEAQIPDLQERVDHILTK
                     QVFGIGITQLTALLARRSVYCSKDATGEHSIAKSFDRDWGNIWFERTEHTWAGGKKTF
                     AVDPLSGEEVVVYANRRCRFCGASETEYDRDDGLETHAYAFIHTNDIKARLNELFGAD
                     MQFDVIIGNPPYQLTTGGGTGTQQAKPLYNLFVERAQELSPRLLCMITPSRWFSGGMN
                     LDGFRSSMLGSHRLRSLVDFPDSRDAFAGVDIAGGVSYFLWDQQHNGPCEVTTVKGSN
                     PVVQHVVRDLDAHDVFVRDNEAVEVLDRVLAAKDFASFSSLVSAISPFGLSTAFRGAR
                     TAAGLIDPVVVRSTVGRQYAERDVIVKNRKLVDEWKVLLSSTSSEHAGQSGKDGRRRV
                     FSRIELAEPGVAMTHSYLAIGPLQDEQQAVNVAVYLRTRFVRFLASLSIITQHVSKGT
                     FGFVPIQDFSTRWTDADLYSKYALTSDEIDFIESQIKAMESDDDQT"
     gene            complement(6731..7357)
                     /locus_tag="VN041_10075"
     CDS             complement(6731..7357)
                     /locus_tag="VN041_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HWU29422.1"
                     /translation="MADERLVKSKQRVADHGEVFTPRWLVDDMLDLVRGETERIDSRF
                     LEPACGSGNFLVPVLERKLAAVQLRHGRSDFEKKHHALFGLMCIYGIELLADNAQECR
                     ENLEGTFARYLKVSADDVWARAARAVLAVNIVQGDALAMTTADGRSIEFPEWGYLGKG
                     RYQRRDFTFENLTQRAAASEGLFAHFDEHDIFTPVREHPVLTVQEIAS"
     gene            7451..7726
                     /locus_tag="VN041_10080"
     CDS             7451..7726
                     /locus_tag="VN041_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29423.1"
                     /translation="MPRVPSPAAVRIGELISDARRRRGITQDELAVLTQIDSSNIRGY
                     EKGRSMMGIPTLIRIADALKVEPGELLNDVTPDMFNVLATDARRRAR"
     gene            7955..9382
                     /locus_tag="VN041_10085"
     CDS             7955..9382
                     /locus_tag="VN041_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWU29424.1"
                     /translation="MTTTARAPKAPDTRGPVRKLLTSFGFQILAALVLGIAAGLIGVS
                     LGASDENHTILSDTLDRIGGSYVTLLKAAVVPLIFTAIVASISNLRRVQNAARLAGQT
                     ILWFAITAFIAVSIGIVLGLVIQPGSKAGEGLQTGEPYAVGTWWNFLLGLIPQNFLGL
                     SVSSSLDPTTNTVTSGVGFNILQVIVVAAAVGIAALKAGKKAEPFLAFTESLLKVIQR
                     VLWWIIRIAPLGTFGLIGSAVIKYGWDKLTALAWFAGAIYIGLAIVLFVVYPILVKTH
                     GLSIKQYFSGVWPAVQLAFVSRSSIGTLPLTERVTERNLGVPSGYASFAVPLGATTKM
                     DGCAAIYPAIAAIFVAQFFDVTLSPIQYLLIVIVSVVGSAATAGTTGAIVMLTLTLST
                     LGLPLEGVGLLLAIDPILDMGRTAVNVAGQALVPTIVAKREGILDLDLYNAPRNGLPF
                     AEDIDEADAADAAEPESGKEPVAAK"
     gene            complement(9446..11158)
                     /locus_tag="VN041_10090"
     CDS             complement(9446..11158)
                     /locus_tag="VN041_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWU29425.1"
                     /translation="MDASIFFVIVGAVAVAAVARWRGWPAPLLVTVVALAAAFLPFVP
                     NPSIDGHVLLNLVLPPLLYSAALDVSFISFTRAMPQIRRLGIGLVVVTTVVVGLVAWW
                     LLPELTLPGALLLGAIVAPPDAVSAAAIGRKLGLPRRVMTVLSGESLINDATSLTLYR
                     VFAATVVTGAAFNFWATIWQFLLAVIVGVLIGLVFGLFMHQLRMRSDDAVINGTVGLL
                     APFGAYAIAEQLGGSGVIAVVAMGLYVGFNAPRTSYTTRQQEAPLWLSADFLLESFVF
                     AYIGLQLPRVVADLSDGEVWPVIGLSAVVLLVVLVVRPLFVFPASAWGQWWTRMRLRK
                     WERMKNGGSGVPVTVERRGRRALTEEQLRQRLTETKLSWRDNAVISWAGMRGVVTLAT
                     ALAAADIAQMDAQAAHAIVVVAFVVTVGTLLLQGLTLPWLIRVLRVVDDREEQEDARQ
                     LAAVRERSREAGKAYLRDMRKQWAQEQGEDQLAAFDAFAKRLIRVESDSETTQIDLRP
                     RPTQEQFMAMAKGWLAVRRRLLLDERDAGNLSEEVMHELITAMDAEELALDTRAATRP
                     QSRA"
     gene            complement(11229..>11539)
                     /locus_tag="VN041_10095"
     CDS             complement(11229..>11539)
                     /locus_tag="VN041_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWU29426.1"
                     /translation="SRMLREGRAAEPIEGRVAIIVDDGIATGATMRTACAIARARGAS
                     HVVVAVPVAAPDALDQLTDADEVVCLCAPRDFMAVGMYYTDFRQTPEEEVVRLLAAAR
                     "
BASE COUNT         1972 a         3934 c         3598 g         2035 t
ORIGIN      
        1 gccctcgcgc gcgatcagcg ctgccaggat cgcggccttc gcgcgggcgt cgccgagcgc
       61 gggccgcgcc gggtcgtagt cgatgtcgag cacggcgcac acgtcaccga gtcgtgagac
      121 cccgaaggcc ctgtgcgccc acagcatggt gtcgagcgcg tcgaacgtcg cagggagcac
      181 gaagccgtcg gacgccagct cggacttgag gaatcgcagg tcgaactcct ggttgtgcgc
      241 cacgagcgtg cgcccgctga gcaggccgac caggtgcgag gcgacttcgc cgatcagcgg
      301 tgcgccggcg agatcctcgg atcggatgcc gcggacctgg gcgcgacctg cgtcgcgctg
      361 cgggttcacg agcgtggtcc actcgcgctc cgggcgtccg gcctcgtcga cgagcacgac
      421 cgcgagctcg atgatgcggt ccccgcgagc cggcgagaag cctgtggtct caagatcgat
      481 gacggcgtag gccattggat gttccccctg ttttccggtt cccgagcggg cgtggacggg
      541 ccgacgcggc gctcgggagg cctcttcgat attggcagac cgcgggaagg atggtggggc
      601 gcctcggatc aggcgaagaa gtcctcgtcg atctcgatgg ccttgaaggt ctcgtacgtc
      661 tggacgccaa cgccgtatcg gatcatgagc cgcgcgaggc gactgccgtc gatcaggatg
      721 atgcgcgtcg gcacggcctc ggcatagttt cgcgccccct ccgagaaacg gctggtggtg
      781 atgaacacgc cgctgtcggc cttgcctgcg agcgccccca cgaatccttg gatatcggga
      841 cgctgcacca cattgtcgga tgcatagcgc ttggcctgga cgtagacgcg actcagtccg
      901 agcacgtcct gatcgatgac cccgtcgatg ccgccgtcat tgctcagctg cgtgaccgtt
      961 ccggtgccgt tcgtgcctcc gtagcccata gcgagcagca agtcgacgac cgcttgctcg
     1021 aagaagccgg gttcctttcc ctggaggcgc gagatcagct cggatgccac ctcgtcgtgg
     1081 atccgctcga tcccctgttg cacctgctcg ataggggtca tcgactccgc cacgtcgccg
     1141 gtcgtcggcg tggatgcctt cgcccgctca gtcttctcgt acacgcgaat gggggagctc
     1201 ggatcctcgc caagttcgcg gatgtcgcgc tccttgacgc catccgggaa cagctcgatg
     1261 agctgtcggc cggcgtcggt gatggtgtag ttcccccgct tgggacgatc gagagcgccg
     1321 acattggtca ggaatgagac accccagccg attctgttct cgtacttgag ttggctcccc
     1381 gacgggagca gctccgtctt ctgttcgtct gtcagtttca cgagatcggc aaccagcggc
     1441 tggaactcgc gccaatggcg aaccaccccg tcgctgagca cacgtaacgt cgggatcatg
     1501 aagccctccc agttgggcat ctcgtcggtc acttgacgtc ctccttgcga tggcgctaag
     1561 tgctccacga gtgatcggac gcccacgcgt cacagcgacg cgccgggcca ccccctctga
     1621 gtagcactac tcgaatactg gcagaggtcg gcgacacggg tagccgctac gcgcggccga
     1681 ctctggccgc gcgcacgtgc gtaggcagtt cagttgccga cgatgtgatg cgcgtgccga
     1741 cccgcgaacc agagtccggt cgcccgagaa cgccggaagg ttccgaggaa gccgaccggt
     1801 ttgacgtgca catcgacgcg ccaactacgt ccgccagaga gcgtcggcag ctcgcttccc
     1861 aaccgcatga gcgcggcgga ccatccggaa accgtctggc tgatgagcac ggcggagcgg
     1921 gatcctggat caactccgat cagccgagcg tcggcatgtc ccgtcgcagg gcggccgatg
     1981 tgctcaccgc cctggaggtg catctggtcg ggacgcaacg agaggtcgtc gacgtcgcgt
     2041 tgggctcgga tcgtgctcaa gcgtgcgcgc cagtcctcgg agagctcaag gggctgatcg
     2101 tcgacgacat gctcgaacgc atcgaagacg ccgatgtgcg ggtgctccac gcaccaagac
     2161 aggtagttct cgagcgtctc gtcgccgaac cacacatcgc gaatcaatcc gaatcgtgcg
     2221 tctgcggccg gcgctccctg atcgagccac cagatgagcc ccttgtcgtg ccgcgtccag
     2281 cccaacgaat acgtgaggaa ggattgaagc gccgcgaaat agccgatggc gtgatcgaag
     2341 tcgccttcga catgtccata ctcgttgcgc tggagcagag cacgcgggct ctgcggcatg
     2401 acagcgccac tcgacggcgc ggcgagcgcg tggtacggca ggcccgtggc ccagacccac
     2461 ggatgctcgc caccgagcgc ctcgtcgtcc gctgagccga caatgacggc ggggcgcgtg
     2521 atccgctgaa tcgggacggc cgctggcatc actcgaccgc gacgaccgtg tcgtaaagcc
     2581 cgtaccgccg aagaccctcg tgcgactcga tgccggtgta cgagagcgaa gcgtcctcat
     2641 atcgccggaa ggcgaacacc gcctggttca tgtggctggc gttgaatact ccgagaccga
     2701 cgagcgtatt gaagtcgtcg acggtgagtc cggtgacggc ctggaagagt ccgggctcga
     2761 tcttcgtgat gacgtcggtg agggtctgct cgcgatagtc cgtgaggtac atgaacgcgg
     2821 gaatccgggt ggcgaacttg atcagcttct cctggatctg cttccgcttg gacttgtact
     2881 ccttctcttc ctcactcagt tgcttcttct cggcggtgtc gagatcgtcg cctttctcct
     2941 tcttcgtctt cgacaccgct tcgctcttgt tcacaacggt ctcgaagaca tccgacccga
     3001 gcgcgcggaa gccctcgatg ttcatcacgg cttccatcgc cttctcattc gccatgatct
     3061 tcttcagagt caggttgtcg acgttcacca gcagcgccga ctcccacttg cgcgccagaa
     3121 gggtcgctga ggtgcccgac atggcaatgt cgaggatgcc gccggcatcc acctgcgtca
     3181 tgttcgaacc gtcatacgcg agtacgggca ggaatttcac cagctcctcc accgcgcgct
     3241 cggggttctt ctcctccggc gcgagtcccg tgccgtactc actcacctgt cgcagcgccc
     3301 tcgtcggcgc gaaatcgaag acgaagcaga ccggcttcag gatctcctcg gcattcgggt
     3361 cgtcaccctc cgggttgcgg atggtccatg gggactggac ccggaacgct gcctggaagt
     3421 agctctcggg cgcctgcaga ttgcgcaaca tgaggatgga cgaccacggg ggcaccgtga
     3481 caccggtagt tagcttgccg acggtgagcg tgatcgtctt cgtgtcgaat cccgcgccga
     3541 tcgccttgcg caccggcggc aatgcatcca gccccacgcc cacgcccggg gctgacacat
     3601 tcacgacgcg gtagtcgtgc cagaagacgt tgtgcttctc ggcgagcagg ttggccatgg
     3661 cggcgcaaga tgctgtgcgc ggaagcatcc agatcgagtg ctgcagatag ggcagcagcc
     3721 tcgtgtcgga gtacgggaac ggcggcttcg tcccagcctt gagcgcgtcg agttgtgtcg
     3781 gtgcgtatga cccacggatg atgtcgagcc acttctgcac gtcgtccttg tgcacgaatg
     3841 cggcatcggc tccggtgccc ttcgcctcga agaaggcatt cagatcgaac tcatcgaact
     3901 caccctgact cgctatcgat atcagttcgt cgggcatctg atacgtcagc agtcgcatct
     3961 ccggcagtga tgcgtacggg ttcttctgat ctggatgctg tgaagcgaac tcggccttcg
     4021 cgcgctgttc gtcggtgtag gtccagttga agatctgctc ctcgatgaat cgaccgtcct
     4081 tgagcacctt gaaaggcgtg cccgacaggt acaggtatgc accggcgcgg atcggaacga
     4141 aggcgtcttc atcgaagccg ttctcctcga gctcctcggc gaaggtctcc aggtctgcac
     4201 cgaactcctg tgcggtcgcc ttcttgcggg tcgcgtcatc ctcgccgtcg aacagttcct
     4261 tggccgtgtc gcgccaggca ccgaagtggt actcgtcgaa gacgaccagg tcccagtgca
     4321 ggtcacgcag ccagtcatgc ttggccttga agttccccgc cttgtcgcgc cccatgaggt
     4381 cctggaagga accgaagaag acgagcggat gctcgggatc gatgtcccgg ggatcaccgc
     4441 cgatcttccg cgaaaggtat tgccagccgt cgaaatcgac gtgcgtcttc aggtcagtgt
     4501 cccaggcgtc ttcgaccgcg ggcttgaagg tcatgacaag tacgcgcttc gcgcccatct
     4561 tcttcgccag ctggtaggtg gtgaaggtct tgccgaagcg catcttggca ttccagagga
     4621 actcaggtgc gtcatcagcg ttgtctgccc agatcgactg gaagtagtcg taagtcttgt
     4681 cgaccgctgc ctgttgctcg gcgcgcatct cgaacgtggc gtggtgagta cctgtgtagc
     4741 gcaggccgtc acggagctcg gctatcgcgg cacggacgtc atcgatcgca caggccatcc
     4801 actcgagcgt gggattgtcg aagcccttct ccttcagccg ggcacgcacg tcatgatcgg
     4861 tgaacagtgt tccgtcgtgc cgctcggccg actcgtccag ctcgattgtg tagttcgtga
     4921 tcgccgcggt cttcgtctgc tgcgcgacgc gcgttttcac gccctgtgtc gtctgaccga
     4981 ctttgagcca tccagagtgc gcgtcgtccg cgatcgagta cgcgtagatc cgcaggtgcg
     5041 cctcgggctt cgcagggagg agttcggatt caggtctggt catcgtccga ctccatggct
     5101 ttgatctgag actcgatgaa gtcgatctca tctgaggtga gcgcatactt cgagtagagg
     5161 tccgcatccg tccatctggt cgaaaagtcc tgaatcggaa cgaagccgaa tgtgcccttc
     5221 gacacgtgct gcgtgattat tgacagcgac gcaaggaatc gcacgaatct tgtgcgcagg
     5281 taaacagcga cgttgaccgc ctgctgctcg tcttggagag gaccgatcgc gagataggaa
     5341 tgagtcattg caacgcccgg ctcagcgagt tcgattcgcg agaagacccg ccgccgcccg
     5401 tctttgcccg attgcccggc gtgctcgctc gaggtggatg acagcagcac cttccactcg
     5461 tctaccagtt tgcggttctt gacgataacg tctcgctcgg catactgcct gcccaccgtc
     5521 gatctcacga cgaccggatc aatcagccca gctgcggtcc tggctcctcg gaaggctgtg
     5581 gagagcccaa agggtgatat cgccgagacc aatgaggaga aggaagcaaa gtccttcgcc
     5641 gcgagaacac gatccaacac ctcgacggct tcgttgtcac gcacaaagac atcgtgtgcg
     5701 tcaaggtcgc gcacgacatg ttgcaccaca gggttgctgc ccttaacagt ggtcacttcg
     5761 catgggccat tgtgttgctg atcccaaagg aagtaggaca cgcctccggc gatgtcgaca
     5821 cctgcgaacg catcgcggga gtcggggaag tctacgaggg atcgcaacct gtgtgagccg
     5881 agcatcgagg agcggaatcc atccaagttc atgccgcccg agaaccatcg cgacggcgtg
     5941 atcatgcaca gcagacgggg ggaaagctcc tgcgcccgct ccacgaaaag gttatagagc
     6001 ggcttcgcct gctgcgtgcc agtacccccg ccggttgtca gctggtacgg tgggtttccg
     6061 atgatgacgt cgaactgcat gtcggctccg aacagctcgt tcagacgagc cttgatgtcg
     6121 ttggtgtgga tgaaggcgta agcgtgggtc tcaagaccgt catcgcggtc gtactcggtt
     6181 tcgctcgcgc cacagaatcg gcatcgccgg tttgcataga ccacgacctc ttcacccgag
     6241 agcgggtcga cggcgaaggt cttcttgcca cccgcccacg tgtgctcggt ccgctcgaac
     6301 cagatgtttc cccagtcgcg gtcgaagctc ttggcgatcg agtgctcgcc ggtggcatcc
     6361 ttcgagcagt acaccgaccg cctcgcgagc agcgcggtga gctgcgtgat cccgatgccg
     6421 aagacctgtt tcgtgagaat gtggtccact cgctcctgaa ggtcggggat ctgcgcttcc
     6481 agcccgtcag tgaggcggcg cgtgatctcg cggaggaaca ctcccgattt cgtgaacgga
     6541 tcgaggaagg tcacggtcga gtccgcccag atggacgcgc cgccgttcga ctcagcccag
     6601 gaccgctcaa gcgtgtcgag catctgattc gcgagctcgg gcggcgtgaa cacctcgtca
     6661 ttcgagagat tcgcgataca cgtgagcaca tccgggttgt gcgactgcag cgcgaatgac
     6721 gccttggcgc tcacgatgcg atctcctgaa cggtcagcac cgggtgctcg cggacggggg
     6781 tgaagatgtc gtgctcgtcg aagtgcgcga agagtccctc tgaggcagca gcgcgctgcg
     6841 tcaggttctc gaaggtgaag tcgcggcgct ggtaccgtcc cttgccgaga tatccccact
     6901 cggggaactc gatggaccgg ccgtccgccg tcgtcatcgc gagcgcgtcg ccctgcacga
     6961 tgttcaccgc aagcaccgcc ctggcggctc gcgcccagac atcgtcggca ctcaccttca
     7021 gataccgggc gaaagtaccc tccaggttct cgcgacactc ctgcgcgttg tcggccagca
     7081 actcgatgcc gtagatgcac atgagcccga acagcgcgtg gtgcttcttc tcgaagtcac
     7141 tgcgaccgtg cctcagctgc accgccgcga gcttgcgctc cagcacgggc accaagaaat
     7201 tgcccgaccc gcaggcgggc tcgaggaacc gcgagtcgat ccgctcggtc tcccccctga
     7261 cgagatcgag catgtcgtca acgagccatc gcggcgtgaa cacctcaccg tgatctgcga
     7321 ctcgctgctt cgacttgacc agacgttcgt cggccacgtg cacctcccag gtatccctca
     7381 cccggagcgg tacaagtacc cgggtgcgtt aaccctggca caactcaccg acattcatga
     7441 cgcttgtcag gtgccccgcg tcccttcacc cgctgctgtc cgcatcggtg agctgatctc
     7501 agacgctcgg aggcggcgcg gcatcactca ggacgagctc gcggtgctga ctcagatcga
     7561 ctcatcgaac atccgcggat atgagaaggg gcgctcgatg atgggcatcc ccacgctgat
     7621 ccgcatcgcg gatgctctga aggtggagcc cggcgagctg ctgaacgacg tgacgccgga
     7681 catgttcaac gtgctcgcca cggacgcacg gcgccgcgcg cgctgatcgc tcatctcgta
     7741 gcggcgccgc tgcgacggac ccagcgaccc gggccggaca aggactccga cgctcgactc
     7801 gagccgcgtc acgcgctcaa tgaccgcgaa gtcgaaatgt gacgtcacgc agtgtcacaa
     7861 gatccccgga attccgcggc gcgccctaac gttgggcaac atggcgcgca tggtgcgcgc
     7921 atcgcccctc cccgctcgac cacatggagc ccctgtgacc accaccgccc gcgcgccgaa
     7981 ggcgcccgac acccgaggcc cggtccgcaa gctgctgacc tccttcgggt tccagatcct
     8041 cgcagccctc gtcctcggca tcgccgccgg cctcatcggc gtctccctcg gcgccagcga
     8101 cgagaaccac accatcctct ccgacaccct cgaccgcatc ggcggctcgt acgtcacgct
     8161 gctgaaggcg gccgtcgtcc cgctgatctt caccgcgatc gtcgcgagca tctccaacct
     8221 gcgccgtgtg cagaacgccg cacgcctcgc cggccagacg atcctgtggt tcgcgatcac
     8281 ggctttcatc gcggtgtcca tcggcatcgt gctcggcctg gtcatccagc ccggcagcaa
     8341 ggccggcgag ggcctgcaga ccggcgagcc gtacgcggtc ggcacctggt ggaacttcct
     8401 gctcggcctg atcccccaga acttcctcgg cctcagcgtc tcgagctccc tcgacccgac
     8461 cacgaacacc gtcacctcgg gtgtcggctt caacatcctg caggtcatcg tggtcgccgc
     8521 cgccgtcggc atcgccgcgc tgaaggcggg caagaaggcc gagccgttcc tggcgttcac
     8581 cgagtcgctg ctcaaggtca tccagcgcgt gctgtggtgg atcatccgca tcgccccgct
     8641 cggcactttc ggcctgatcg gctccgccgt catcaagtac ggctgggaca agctcaccgc
     8701 cctggcctgg ttcgccggcg cgatctacat cggcctggcg atcgtgctgt tcgtcgtcta
     8761 cccgatcctc gtgaagacgc acggcctgtc gatcaagcag tacttctcgg gcgtgtggcc
     8821 ggccgtgcag ctcgcgttcg tcagccgctc ctccatcggc accctgccgc tcaccgagcg
     8881 cgtcaccgag cgcaacctcg gagtcccctc cggctacgcc tcgttcgccg tgccgctcgg
     8941 cgcgaccacc aagatggacg gctgcgccgc gatctacccg gccatcgccg cgatcttcgt
     9001 cgcccagttc ttcgacgtga ccctcagtcc catccagtac ctgctgatcg tcatcgtctc
     9061 ggtcgtcggc tccgccgcga ccgcgggcac caccggcgcg atcgtcatgc tcacgctgac
     9121 gctctcgacc ctgggcctgc cgctggaggg cgtcggcctg ctgctcgcga tcgacccgat
     9181 cctcgacatg ggccgcaccg cggtgaacgt cgccggtcag gcgctggtcc cgacgatcgt
     9241 cgccaagcgc gagggcatcc tcgacctcga cctgtacaac gcgccgcgca acggcctccc
     9301 cttcgccgag gacatcgacg aggcggatgc cgcggatgcc gccgagccgg agtccggcaa
     9361 ggagcccgtc gccgcgaagt gacgacgagc ggatgcctga gcgcctcgcc cctcacaggg
     9421 ggcgaggcgc tctcgcattc cggcgtcagg cgcgcgactg cgggcgggtc gcggcgcggg
     9481 tgtcgagcgc cagctcctcg gcatccatcg ccgtgatcag ttcgtgcatg acctcttcgc
     9541 tgaggttccc ggcatcccgc tcgtccagca gcagacggcg tcgtaccgcg agccatccct
     9601 tcgccatggc catgaactgc tcctgggtgg ggcgcggcct caggtcgatc tgcgtggtct
     9661 cggaatccga ctccacccgg atcagccgct tcgcgaacgc atcgaaggcg gccagctgat
     9721 cctcgccctg ctcctgcgcc cactgcttgc gcatgtcccg cagatacgcc ttgcccgcct
     9781 ctcgactgcg ctcgcgcacg gccgccagct gccgggcgtc ctcctgctcc tcacggtcgt
     9841 cgaccacccg cagcacacgg atcagccagg gcagcgtcag accctgcagc agcagggtgc
     9901 cgaccgtcac cacgaacgcg accacgacga tcgcgtgtgc ggcctgcgca tccatctggg
     9961 cgatgtcggc cgcggcgagc gcggtcgcca gcgtgaccac tccgcgcatc ccggcccacg
    10021 agatcaccgc gttgtcccgc caggacagct tggtctcggt cagacgctgc cgcagctgct
    10081 cctcggtgag cgcacgacgg cctcgccgct ccacggtcac gggcaccccc gagccgccgt
    10141 tcttcatgcg ctcccacttg cgcaggcgca tacgcgtcca ccactggccc cacgcactgg
    10201 cggggaacac gaacagggga cgcaccacca gcaccaccag cagcaccacg gccgacagcc
    10261 cgatcacggg ccagacctcg ccgtcgctga ggtcggcgac cactcgcggc agctgcaggc
    10321 cgatgtaggc gaacacgaac gactccagca ggaagtcggc gctcagccac agcggcgcct
    10381 cctgctgcct cgtcgtgtaa ctggtgcgcg gggcgttgaa gccgacgtag agtcccatcg
    10441 cgaccaccgc gatcacgccg gagccgccga gctgctcagc gatcgcgtag gcgccgaacg
    10501 gcgcgagcag tccgaccgtg ccgttgatga ccgcgtcatc gctgcgcatc cgcagctgat
    10561 gcatgaacag cccgaacacc agcccgatca gcacgccgac gatcaccgcc agcaggaact
    10621 gccagatcgt cgcccagaag ttgaaggccg caccggtgac caccgtggcg gcgaacacgc
    10681 ggtacagcgt cagcgaggtg gcgtcattga tcaggctctc tcccgacagc accgtcatca
    10741 ccctccgcgg cagacccagc ttgcggccga tggcggcagc ggagacggca tccggcgggg
    10801 cgacgatcgc ccccagcagc agcgccccgg gaagcgtcag ctccggcagc aaccaccacg
    10861 cgaccaagcc gaccacgacg gtcgtgacga ccaccaggcc gatcccgagc ctgcggatct
    10921 gcggcatggc gcgcgtgaag ctgatgaacg acacgtcgag cgccgccgag tacagcagcg
    10981 gcggcagcac gaggttcagc agcacgtggc cgtcgatcga ggggttcggc acgaacggca
    11041 ggaatgccgc tgcgagcgcg accaccgtga ccagcagcgg cgccggccac ccccgccagc
    11101 gcgcgaccgc cgccaccgcg accgcaccga cgatgacgaa gaagatgctc gcgtccatgg
    11161 gggcaaggct aacgcggcca gtcccgctca tcccggcgcc ggaccgcgct tcgccgagag
    11221 cgccgcgctc accgtgcggc ggcgagcagc cggaccacct cctcctccgg ggtctgacgg
    11281 aagtccgtgt aatacatgcc gacggccatg aagtcacgcg gcgcgcagag gcagaccacc
    11341 tcgtcggcat cggtcagctg atccagtgca tccggcgccg ccaccggaac ggcgaccacc
    11401 acgtgcgacg caccgcgggc gcgggcgatc gcgcatgccg tccgcatggt cgccccggtc
    11461 gcgatcccgt catccacgat gatcgcgaca cggccctcga tcggctctgc ggcgcggccc
    11521 tcgcgcagca ttcgcgacc
//
LOCUS       DATMSX010000325         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_43146,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000325 DATMSX010000000
VERSION     DATMSX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43146"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VN041_10100"
     CDS             <1..518
                     /locus_tag="VN041_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29427.1"
                     /translation="VLVPLLAGMSLAGFATGALSRVLALRTQLVLGALVMGASVIAAV
                     LFHAAVWQLAIVGGVFGIGVGTAYAAMASIIVEGVPASSVGIATGVNANLRSIGSAIG
                     SALMTAIVFGSLDSGGRPFESGYDTAWITIAAVAVLAAVIVAVVPVNRRRDEAPVLLP
                     TAPQPVLADAA"
     gene            complement(600..1889)
                     /locus_tag="VN041_10105"
     CDS             complement(600..1889)
                     /locus_tag="VN041_10105"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWU29428.1"
                     /translation="MSAAQPDKATLSIGDTTAEFPLLPSVSGAGSIDFSTLTRQTGYT
                     GLDYGFVNTASTKSAITFIDGDQGILRYRGYPIEQLAGRTSYLEVAWLLIYGELPTAD
                     ELADFDEKIRRHTLLHEDLKRFFSALPHTAHPMSVLSSAVAALSTYYEGQTDPHDPEH
                     VELNMVRMLAKLPVIAAYAHKKSVGQAFLYPDNSLSFVDNFLKLNFGVLSEEYQVNPV
                     MSKALELLLILHEDHEQNASTSTVRLVGSTGANQFASISAGIQALSGPLHGGANEAVL
                     TMLGQIRESGQSVQRFVERVKNKEEGVKLMGFGHRVYKNYDPRAKLVKEAADEVLAEL
                     GVTDPLLDLAKELEEIALADDYFKERRLYPNVDFYTGVIYKAMGFPTRMFTVLFAIGR
                     LPGWLAQWRELNLDPQTKIGRPQQLYVGSPERAYPGH"
     gene            complement(2049..>2676)
                     /locus_tag="VN041_10110"
     CDS             complement(2049..>2676)
                     /locus_tag="VN041_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU29429.1"
                     /translation="LDVDALRRAVEAAREIGAVVAGDECYAELGWDGPWASEPIPSVL
                     DPRVTGGTRSGLLSVYSLSKQSNLAGYRAALVAGCARIVGDLLTARKHLGLMPPAPVQ
                     HAMAVALRDDEHVAAQKELYRQRRELLVPALTAAGFRIDGSEAGLYLWVTEGRDAWES
                     MGRLADLGILAGPGHFYGDHSADHVRISLTAPLDRVAQAAQRLSASAR"
BASE COUNT          398 a          904 c          937 g          437 t
ORIGIN      
        1 tcgtgctggt gccgctgctg gcgggcatgt ctctggccgg tttcgccacc ggagcgctca
       61 gccgcgtgct cgccctgcgc acgcagctcg tgctgggcgc actggtgatg ggagcctcgg
      121 tgatcgccgc ggtgctcttc cacgccgcgg tctggcagct cgcgatcgtg ggcggcgtct
      181 tcggtatcgg cgtgggcacc gcctacgccg ctatggcgag catcatcgtc gaaggggtgc
      241 ccgcgagcag cgtcggcatc gccaccggcg tgaacgcgaa cctgcgctcg atcgggtccg
      301 cgatcggatc ggcactgatg accgcgatcg tcttcggctc cctcgactcc ggcgggcgac
      361 cgttcgagag cggctacgac accgcctgga tcacgatcgc agccgtcgcg gtgctcgccg
      421 cagtcatcgt ggccgtcgtc cccgtgaacc gtcgccgcga cgaggcgccg gttctcctcc
      481 cgaccgcccc gcagccggtg ctcgccgacg ccgcctgacg tcgctgccga ttcgccggaa
      541 cagacgagaa cggccccggg atcctccaga ggattccggg gccgttctgc atgtcacgct
      601 cagtggccgg ggtaggcgcg ctccggggag ccgacgtaca gctgctgcgg acggccgatc
      661 ttggtctgcg ggtcgaggtt cagctcgcgc cactgcgcca gccaaccggg caggcgaccg
      721 atcgcgaaca gcacggtgaa catgcgcgtc gggaagccca tggccttgta gatcacgccg
      781 gtgtagaagt cgacgttcgg gtacaggcgg cgctccttga agtagtcgtc cgcgagggcg
      841 atctcctcga gctccttggc caggtcgagc agcggatcgg tcacgccgag ctcggcgagc
      901 acctcgtcgg cagcctcctt gacgagtttg gcgcgcgggt cgtagttctt gtagacccgg
      961 tggccgaagc ccatcagctt gacgccctcc tccttgttct tcacccgctc gacgaagcgc
     1021 tgcacgctct gacccgactc gcggatctga ccgagcatgg tgaggacggc ctcgttggcg
     1081 ccgccgtgca gcgggccgga cagcgcctgg atgccggcgg agatggacgc gaactggttc
     1141 gcccccgtgg agccgaccag gcgcacggtg gaggtggacg cgttctgctc gtggtcctcg
     1201 tgcaggatca gcagcagctc gagcgccttc gacatcaccg ggttgacctg gtactcctcg
     1261 gagagcacgc cgaagttgag cttgaggaag ttgtcgacga agctcagcga gttgtccggg
     1321 tagaggaacg cctggccgac gctcttcttg tgcgcgtacg cggcgatcac cggcagcttg
     1381 gcgagcatgc gcaccatgtt cagctcgacg tgctcgggat cgtgcgggtc ggtctggccc
     1441 tcgtagtacg tcgacagcgc ggcgaccgcg gaggacagca ccgacatcgg gtgtgcggtg
     1501 tgcggcagcg cggagaagaa gcgcttgaga tcctcgtgca gcagcgtgtg acggcggatc
     1561 ttctcgtcga agtccgcgag ctcatccgcg gtgggcagct cgccgtagat gagcagccag
     1621 gccacctcga ggtagctcgt gcgccccgcc agctgctcga tggggtaccc gcggtagcgc
     1681 aggatgccct ggtcgccgtc gatgaacgtg atcgcggact tggtggaggc cgtgttcacg
     1741 aacccgtagt ccagacccgt gtacccggtc tgtcgcgtca gcgtggagaa gtcgatgctc
     1801 cccgccccgc tgacagatgg cagcagaggg aactccgcgg tcgtgtcacc gatcgagaga
     1861 gtcgccttgt ccggctgagc tgcgctcacg ccaacctcct gctgcgtctg tgtcgtcgtg
     1921 gacgatgtgt ggcgcagagc gcctgccggt gaacggccgc atcgcgttta cagcctagcc
     1981 gcgcctccgg cctactgtga catccaccaa gatatggacg atttcacgcc ggaaaccgac
     2041 aaatcacctc agcgagcgga tgccgagagc cgctgcgcgg cctgcgcgac ccggtcgagc
     2101 ggcgcggtca gcgagatgcg gacgtggtcg gcggaatggt caccgtagaa gtgtcccggg
     2161 cccgccagga tgccgaggtc ggcgaggcgg cccatcgact cccaggcgtc gcgcccctcg
     2221 gtcacccaca ggtacagccc tgcctcggag ccgtcgatgc ggaatccggc cgccgtgagc
     2281 gcgggcacga gcagctcgcg ccgctgccgg tagagctcct tctgcgcagc gacgtgctcg
     2341 tcatcgcgca gagccaccgc catggcgtgc tgcaccggcg ccggcggcat gaggccgagg
     2401 tgtttgcgcg cggtcagcag atcgccgacg atgcgcgcgc atcccgccac gagcgccgcg
     2461 cggtaaccgg cgaggttgga ctgcttgctg agggagtaga cgctgagcag accggagcgg
     2521 gtccccccgg tgacacgcgg gtccagcacg gaggggatcg gctcggatgc ccaggggccg
     2581 tcccagccga gctcggcgta gcactcgtcg cctgcaacga cggcgccgat ctcccgcgcc
     2641 gcctccaccg cccgccgcag tgcgtccacg tcgagc
//
LOCUS       DATMSX010000326         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_33417,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000326 DATMSX010000000
VERSION     DATMSX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33417"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VN041_10115"
     CDS             <1..1190
                     /locus_tag="VN041_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29430.1"
                     /translation="VSQLVTDGRVRLLVGTRGLLGEGWDCPAVNTLIDLTSVATSVGT
                     QQLRGRTLRLDPAWPEKVAHNWSVVCLIPPRVELDDVAELNRLRRRHDHLWGLSADDH
                     ARVITGLGAALDAPAQALLDRIVAKDETATIAQLNEAVRAGMRSRAQILADWRVGEPY
                     EARERDVVAVRSIRRSELVQTLPAVAAGATPSLLFSALIGGGVVGMGSGFLGWDPPLA
                     FGIGAALGALFPASLRLGAITKAVRQQRDPAQTYRAAAQAVGQALQDAGRVPPLPEGA
                     IEVDLQRLLDGSSRLRLAFQGEARTRRMLADAVQELFGPVRTPRFLLRLPGRRSPAFL
                     PVPTQIARRRADAEHFRDLWTQALGPCELIELQGEAGLVTLRAARAQPARLDEVPRTT
                     LWG"
     gene            1270..>2073
                     /locus_tag="VN041_10120"
     CDS             1270..>2073
                     /locus_tag="VN041_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU29431.1"
                     /translation="MTEQSTAQITPEITAERFAEHTSQIIASVSSVIDGKPEAVRSAL
                     TCLFAEGHLLIEDVPGVGKTMLARALAASVDATVRRIQFTPDLLPGDVTGVAVYDPVA
                     REFEFKRGAVFANIVIADEINRASPKTQSALLEAMEEGQVTVDGSTHHLPAPFLVVAT
                     QNPLEMEGTYPLPEAQRDRFMMRISMGYPDAAAETQMLRQRETSNPLAAITPVTDAAT
                     IGALIAWTRRVHVAESLEEYAVALAQATRQDPSLHLGASPRATLQLVRAA"
BASE COUNT          285 a          772 c          716 g          300 t
ORIGIN      
        1 ccgtgtcgca gctcgtgacc gacggcaggg tgcggctgct ggtgggcacg cgcgggctgc
       61 tcggtgaggg ctgggactgc cccgccgtga acacgctgat cgatctgacc agcgtggcga
      121 cgagcgtcgg cacgcagcag ctgcgcggtc gcacgctgcg actggatccg gcgtggcccg
      181 aaaaggtcgc tcacaactgg tcggtcgtct gcctcatccc accgcgcgtg gagctggatg
      241 acgtcgccga gctgaaccgg ctgcgccgcc gccacgatca cctctggggg ctgagcgccg
      301 acgaccacgc ccgcgtgatc accggtctcg gcgccgcgct cgatgcgccc gcccaggcgc
      361 ttctcgatcg catcgtggcg aaggacgaga ccgccacgat cgctcagttg aacgaggccg
      421 tgcgggccgg gatgcgatct cgtgcgcaga tcctcgcgga ctggcgcgtc ggcgagccgt
      481 acgaggccag ggagcgcgac gtggtggccg tgcgcagcat ccgccgctcc gagctggtgc
      541 agaccctgcc cgctgtggcc gcgggcgcga caccgtcact gctgttcagc gcgctgatcg
      601 gcggtggcgt tgtcggtatg ggcagcggct tcctggggtg ggatccgccg ctcgcgttcg
      661 ggatcggcgc cgccctcggc gcgctcttcc ccgcgagcct tcggctgggc gcgatcacga
      721 aggcggtgcg acagcagcgc gatcccgcgc agacgtatcg cgccgccgcg caggcggtcg
      781 gccaggcgct acaggatgct ggtcgtgtcc cgccgctgcc ggagggagcc atcgaggtcg
      841 acctgcagcg ccttctcgac ggctcgtcgc gtctgcggct ggccttccag ggtgaggctc
      901 ggacgaggcg gatgctggcg gatgccgtgc aggagctgtt cggcccggtg cgcacgccgc
      961 gcttcctgct gcgcctcccc ggccgccgct ctccggcgtt ccttccggtc ccgacgcaga
     1021 tcgcccggcg acgagcggat gccgagcact tccgcgacct gtggacgcag gccctcgggc
     1081 cgtgcgagct catcgagctg cagggcgagg cgggtctggt gaccctgcgc gccgcccgcg
     1141 ctcagcccgc ccggttggac gaggtgccgc gcaccactct gtggggctga acgctccccc
     1201 cgttcacctc cccgcgttac gatcagagtt cgcaccgtcg cgcaggggag ccaccgcatg
     1261 ccagagcaga tgaccgagca gagcaccgcc cagatcacgc ctgagatcac cgccgagcgg
     1321 ttcgccgagc acacctccca gatcatcgcc tcggtctcct ccgtcatcga cggcaagccc
     1381 gaagccgtgc gcagcgcgct gacctgcctg ttcgccgagg gccacctgct catcgaggac
     1441 gtaccgggcg tcggcaagac catgctcgcg cgcgccctcg ccgccagcgt ggacgccacc
     1501 gtccgccgca tccagttcac ccccgacctg cttcccggcg acgtgaccgg cgtcgcggtg
     1561 tacgacccgg tggcgcgcga gttcgagttc aagcgcggcg ccgtgttcgc caacatcgtg
     1621 atcgccgacg agatcaaccg cgcctcgccc aagacccagt ccgcactgct ggaggccatg
     1681 gaggagggcc aggtcacggt cgacggctcc acccatcacc tgcccgcgcc cttcctcgtc
     1741 gtcgcgacgc agaacccgct cgagatggag ggcacgtatc cgctgcccga agctcagcgc
     1801 gaccggttca tgatgcgcat ctcgatgggg tacccggatg ccgccgccga gacgcagatg
     1861 ctgcgccagc gcgagacctc caacccgctc gccgcgatca cgccggtgac ggatgccgcc
     1921 accattggcg ccctgatcgc ctggacgcga cgggtgcacg tggccgaatc cctcgaggag
     1981 tacgcggtcg cactcgccca ggccacccgc caggacccga gcctgcacct gggcgcgagc
     2041 ccccgtgcga cgctgcagct ggtccgcgcg gcg
//
LOCUS       DATMSX010000327         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_128392,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000327 DATMSX010000000
VERSION     DATMSX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128392"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VN041_10125"
     CDS             complement(<1..413)
                     /locus_tag="VN041_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWU29432.1"
                     /translation="MSGVPIYPTRGRGGGWRLIGGARTDLTGLTEGEVTSLLIGLTQS
                     LGAEPERVAAVRKLVRAMPAPFREGAQRVADATVRDVPWGEVQPASVSETVTTLQRAI
                     AARRQVRIQYEGAQGVVTRELVPLVVGSRGARWYLL"
     gene            651..1046
                     /locus_tag="VN041_10130"
     CDS             651..1046
                     /locus_tag="VN041_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU29433.1"
                     /translation="MFRGLANLNLVAVDMDAAVEWYSAVFESAPYFVRPEQGPAQYAE
                     WRFGDDEDEFALMDARFRPALPQPGGALVSMHVDDIQASFDRLIGLGAVALDPVTQRG
                     EGWWSASVSDPFGNLIGLIQSPHWAAQHA"
     gene            complement(1115..2149)
                     /gene="nusG"
                     /locus_tag="VN041_10135"
     CDS             complement(1115..2149)
                     /gene="nusG"
                     /locus_tag="VN041_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWU29434.1"
                     /translation="MSERYSDDADWATAAEQSSEEDEAQEGNILAEQEQSVASAETVA
                     LHLEGGDDSDEEYEDDIDDIDIEDPEADAIVNDALNLDEAAESEAAAEVLNDAVAEDT
                     AEEAAAAAEEVTPYDGPELGADEESDDDEAAEAAEEDPYEAFRMDLRMLPGKWYVIHS
                     YAGFERKVKANIEQRKSTLEVEDEIYQIEVPMEDVVEIKNGQRKMVTRVRIPGYVLVR
                     MELNEDTWSVVRHTPGVTGFVGNAHNPTPLRFEEAFNMLKSLVEVKDVPTAKSIAAKG
                     GVAVARPMAAEVDFEVGETITIKEGSFAGLPGTISEINAAAGKLTVLVSLFERETPVE
                     LSFEQVTKML"
     gene            complement(2166..2453)
                     /gene="secE"
                     /locus_tag="VN041_10140"
     CDS             complement(2166..2453)
                     /gene="secE"
                     /locus_tag="VN041_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWU29435.1"
                     /translation="MDQDDVRGDAVAMGPYSLRDKQLGFFGRIALFFRQVMSELRKVV
                     TPTRKELIKFTLVVLVFVLIVMGLVYGFDSLFGFVTHLVFGVPGTAPAPAQ"
     gene            complement(2495..2567)
                     /locus_tag="VN041_10145"
     tRNA            complement(2495..2567)
                     /locus_tag="VN041_10145"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2532..2534),aa:Trp,seq:cca)
     gene            2792..>4201
                     /locus_tag="VN041_10150"
     CDS             2792..>4201
                     /locus_tag="VN041_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HSP90 family protein"
                     /protein_id="HWU29436.1"
                     /translation="MSSDVQQFQVDLRGVVDLLSRHIYSSPRVYLRELLQNARDAIAA
                     RSEVDGAGGSIRISPLTDETGEFVLRDDGVGLTASEVTELLATVGRSSKRDIFDLPRS
                     DYLGQFGIGLLSCFMVSDRIVIRSRSARGGASVEWVGSSDGTFQVVELDEELPVGTSV
                     HLAPRFDQADLLRPAAVRELARTFAEFLPVRVTVEMPGGDVEITRPAPFLGEGGPDAV
                     AEYGRDLIGATPMDVIEISEPGTGTRGIAYVLPFAPPPNVRQATRMYLGRMLLAERAP
                     DVLPDWAFFVRAVVDSQTLSPTASRESLVEDAALEHVREQLGAGIRRWVLELGLTAPH
                     LLTQFVAVHELGLKSLVRHDEELARFITRWLTVETTHGTIRLGDLVERYPHVRYARTV
                     DEFRQVAGIIPASEVLVNGGYLYDADLIAALPELHREVTVEQIDVSAELDRLDPPPLD
                     DRDAAIALEDRASAVLAASG"
BASE COUNT          642 a         1467 c         1407 g          685 t
ORIGIN      
        1 agcagatacc agcgcgcgcc gcggctgccg acgacgagcg gcaccagctc gcgggtaacg
       61 acgccctgcg ccccttcgta ctggatgcgc acctgtcgcc gcgcggcgat ggcccgctgc
      121 agtgtggtca cggtctcgga cacggatgcc ggctgcacct cgccccacgg gacgtcgcgc
      181 accgtggcat ccgccactcg ctgggcgccc tcccggaacg gcgcaggcat ggcgcgcacg
      241 agcttgcgga ccgcggcgac ccgctcgggc tccgcgccca ggctctgcgt caggccgatg
      301 agcagcgagg tgacctcgcc ctcggtgagg ccggtcagat cggtgcgggc gccgccgatc
      361 agccgccacc cgccgcctcg cccgcgagtg gggtagatcg gcacgcccga catcgccagc
      421 gcctcgaggt cccgtcgtgc ggtcggaacc gagacctcca gctcgcgcgc cagctcggca
      481 gcggtgacct ggggtcgccc ctgcagcagg agaagggcct ggatgaggcg gtcggcgcgg
      541 atgcggaagc tcgattcgga agattcttca gaaggatttt taagtgatca gaagatgatc
      601 acttcagatt cgagcataga aggacaagca cctcagcaga aggagaaatc atgttccgtg
      661 gactcgccaa cctcaacctc gtcgccgtcg acatggatgc cgccgtcgag tggtactcgg
      721 ccgtgttcga gtcggctccc tacttcgtcc gccccgagca gggccccgcc cagtacgcgg
      781 agtggcgctt cggggatgac gaggatgagt tcgccctgat ggacgcccgc ttccgtcccg
      841 cgctccccca gcccggcggc gccctggtca gcatgcacgt cgacgacatc caggcctcgt
      901 tcgaccgtct gatcggcctc ggcgccgtcg ctttggaccc ggtgacccag cgcggcgagg
      961 gctggtggtc ggcatccgtc agcgacccgt tcggcaacct gatcgggctg atccagagcc
     1021 cgcactgggc cgcgcagcac gcctgaacac gctcgacaga cacgaaagaa cggccgcccc
     1081 ggaagggacg gccgttcttc gtacgcgtga agcgtcagag catcttggtg acctgctcga
     1141 acgacagctc gacgggggtc tcgcgctcga agagcgagac gagcacggtg agcttgcctg
     1201 cggcggcatt gatctcgctg atcgtgccgg gaagacccgc gaacgagccc tccttgatcg
     1261 tgatggtctc gccgacctcg aagtcgacct cggcggccat cggacgggcc acggccacgc
     1321 cgcccttggc ggcgatggac ttcgcggtgg ggacgtcctt cacctcgacc agcgacttga
     1381 gcatgttgaa cgcctcttcg aagcgcagcg gcgtcgggtt gtgcgcgttg cccacgaagc
     1441 cggtgacacc cggcgtgtgg cggacgaccg accaggtgtc ctcgttcagc tccatgcgca
     1501 cgagcacgta gcccgggatg cgcacgcggg tgaccatctt gcgctggccg ttcttgatct
     1561 cgacgacgtc ctccatcggg acctcgatct ggtagatctc gtcctcgacc tcgagcgtcg
     1621 acttgcgctg ctcgatgttc gccttcacct tgcgctcgaa gccggcgtag gagtggatga
     1681 cgtaccactt gccgggcagc atccgcaggt ccatccggaa ggcctcgtac gggtcctcct
     1741 cggcagcctc ggcggcctcg tcgtcgtcgg actcctcgtc ggcgccgagc tcggggccgt
     1801 cgtagggagt cacttcttca gcggcggcag ccgcctcctc ggcggtgtcc tccgcaacag
     1861 cgtcgttgag gacttcggcg gctgcttcgg actcagctgc ttcgtccagg ttcagagcgt
     1921 cgttcacgat cgcgtccgcc tccgggtctt cgatgtcgat gtcgtcgatg tcgtcttcgt
     1981 actcctcgtc ggagtcgtcg ccaccctcga gatgcagggc gacggtctcc gcggacgcca
     2041 ccgactgctc ctgctcggcg aggatgttgc cctcctgggc ctcgtcctcc tcgctggact
     2101 gctcggccgc ggtggcccag tcggcgtcgt cggaatatcg ttcagacacg ttgcttactt
     2161 tccgttcact gcgcgggcgc aggtgcggtg ccgggaactc cgaacaccag gtgggtcacg
     2221 aagccgaaca gcgagtcgaa gccgtagacc agccccatca cgatcagcac gaacaccagc
     2281 accaccaggg tgaacttgat cagctccttg cgagtcggcg tgacgacctt gcggagctcg
     2341 ctcatcacct gccggaagaa cagcgcgatg cggccgaaga agccgagctg cttgtcccgc
     2401 agggagtacg ggcccattgc cacggcatcg ccgcgaacgt cgtcctgatc catcccgcaa
     2461 gtacctttcg tgagtcagca tgtgcgctga cgcgcagggc ggacaggaat cgaacctgca
     2521 acctgcggtt ttggagaccg ctgctctgcc aattgagcta ccgccctaga gaccggatgg
     2581 tccctgggtc cgcgtcctcc gtgtgccgat cagggcacgg tgaaggatgc agacaactgc
     2641 ccctcaagtg tacggcatcc gatcggctgg gccgaaccga gccgacatcc cgcgtgagtc
     2701 gcgcacttcc gcagcccgcc ggatgcggat catcgcccgc acttcgggca gacttgaggg
     2761 cactgatttc cacacggccg gagggggtcc cgtgagttcg gacgtgcagc agttccaggt
     2821 cgatctgcgc ggtgtcgtcg acctgctcag caggcacatc tactcgagtc cgcgggtgta
     2881 cctgcgcgag ctgctgcaga acgcgagaga cgcgatcgcc gcgcgctccg aggtcgacgg
     2941 cgcaggcggc agcatccgca tctcccccct caccgacgag accggcgagt tcgtgctgcg
     3001 cgacgacggc gtcggcctga ccgcgtccga ggtcaccgag ctgctcgcca ccgtcggacg
     3061 cagctccaag cgcgacatct tcgaccttcc gcgcagcgac tacctcgggc agttcggcat
     3121 cggcctgctc agctgcttca tggtgtcgga ccgcatcgtg atccgctccc gcagcgcccg
     3181 cggcggtgcg agcgtggagt gggtgggcag ctccgacggc accttccagg tggtcgaact
     3241 ggacgaggag ctgccggtcg gcacgagcgt gcacctggcg ccgcgcttcg atcaggccga
     3301 tctgctgcgc ccggccgccg tacgggagct ggcgcgcacc ttcgccgagt tcctgccggt
     3361 gcgggtgacc gtggagatgc cgggcggcga cgtcgagatc acccgtccgg cgccgttcct
     3421 cggcgagggc ggaccggatg ccgtggccga atacggccgc gacctcatcg gcgcgacgcc
     3481 gatggacgtg atcgagatct ccgagcccgg caccggcacg cgcggcatcg cgtacgtgct
     3541 cccgttcgcc ccgccgccga acgtgcggca ggcgacccgg atgtacctcg gccggatgct
     3601 gctcgccgag cgagcccccg atgtgctgcc ggactgggcg ttcttcgtgc gcgccgtggt
     3661 cgactcgcag acgctgtcac ccaccgccag ccgggagtcg ctggtggagg acgccgcact
     3721 cgagcacgtg cgcgagcagc tcggcgcggg catccgccgc tgggtgctgg agctggggct
     3781 gaccgcaccg cacctgctca cccagttcgt ggcggtgcac gagctgggcc tgaagtcgct
     3841 ggtgcggcac gacgaggagc tcgcccgctt catcacccgc tggctcaccg tggagaccac
     3901 gcacggcacc atccggctcg gagacctcgt ggagcggtac ccgcacgtgc gctacgcgcg
     3961 caccgtggac gagttccgac aggtggcggg gatcatcccc gcatccgaag tgctggtcaa
     4021 cggcggatac ctgtacgacg ccgacctgat cgccgcgctc ccggagctgc atcgcgaggt
     4081 gaccgtcgag cagatcgacg tgtcggcgga gctcgaccgc ctcgacccgc cgccgctgga
     4141 cgaccgcgac gccgcgatcg cgctggagga ccgcgcgagc gccgtgctcg cggcatccgg
     4201 c
//
LOCUS       DATMSX010000328         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156788,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000328 DATMSX010000000
VERSION     DATMSX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156788"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN041_10155"
     CDS             <1..185
                     /locus_tag="VN041_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29437.1"
                     /translation="DLSPGLDGVPAPDASDAEWVRPELVGEVEFANWSPSGILRHSRW
                     RGLRPDKSPEEVRRES"
     gene            complement(186..1106)
                     /locus_tag="VN041_10160"
     CDS             complement(186..1106)
                     /locus_tag="VN041_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWU29438.1"
                     /translation="MHDHAPAPGDIRDAGHRRLLAVSLGLTATIMVVQVVGAILSGSL
                     ALLADAAHMFTDSSALVIALIATAVAARPADDRRTFGYQRAEVFAALINAIILIVLTV
                     SVAIGGVNRLLNPGEVEVAGPVMLAVAVVGMLANGTSMWLLSRAQEKSINVRGAYLEV
                     MGDLIGSVMVIIAAIVIVTTGWMPADAIASLVIAAMIVPRAISLLREVFSVLAESAPK
                     GMSVDDIRTHLKGYEGVVDVHDVHVWQLTRGAPVFTAHVSVAQSVYAQGRSAELLTRL
                     QSCLADHFDVEHSTFQLEPAGHDDCEATHA"
     gene            complement(1185..1778)
                     /gene="rdgB"
                     /locus_tag="VN041_10165"
     CDS             complement(1185..1778)
                     /gene="rdgB"
                     /locus_tag="VN041_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWU29439.1"
                     /translation="MKVVLATHNPHKVAEFQQIVAATRPDLEVIGYDGPEPVEDGVTF
                     AENALIKARAAAAHTGLPALADDSGICVDVLGGSPGVFSAYWAGQKKDATANLELLLD
                     QLRDIADPHRAAQFHSTIALVTADGTERTVQGIWPGRLAHSPAGPGGFGYDPIFIPDG
                     QDAASERTVGEFTDAEKQSQSHRARAFQALIPLLAEL"
     gene            complement(1775..2515)
                     /gene="rph"
                     /locus_tag="VN041_10170"
     CDS             complement(1775..2515)
                     /gene="rph"
                     /locus_tag="VN041_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWU29440.1"
                     /translation="MSDIVRADGRSTDQFREVTIERGWSAHAEGSALVSFGGTKVLCT
                     ASFTNGVPRWLTGKGKGWVTAEYAMLPRATNSRNDRESVKGRIGGRTHEISRLIGRAL
                     RAVVDTKALGENTIVIDCDVLQADGGTRTAAITGAYVALADAIEWGREKKFIGKNSTP
                     LLDSVAAVSVGIIDGEPMLDLAYVEDVRAETDMNVVVTGRGLFVEVQGTAEGAPFDKR
                     ELDALLELGVNGCASLREHQLASLAAAR"
     gene            complement(2544..3398)
                     /gene="murI"
                     /locus_tag="VN041_10175"
     CDS             complement(2544..3398)
                     /gene="murI"
                     /locus_tag="VN041_10175"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWU29441.1"
                     /translation="MNDAPIGIFDSGVGGLTVARAIRAQLPQESLVYIGDTAHSPYGP
                     KPIADVRRYSLEVLDTLVDQGVKMLVIACNTASAAMLRDARERYDVPVVEVIGPAVRR
                     AVSTTRNGRIGVIGTVGTIGSRAYQDMLEVNERLEVFTAACPRFVEFVEAGITGTPEV
                     LAVAEGYLAPLRDAEVDTLVLGCTHYPFLRGAISYVMGDGVSLVSSDDETAADVYRQL
                     VRHDQLAPADAVPTYTYEATGASTEDFTVLANRLMGREVRDVQLVQTGVITLPDLSGV
                     DGADALRP"
     gene            complement(3410..4333)
                     /gene="murQ"
                     /locus_tag="VN041_10180"
     CDS             complement(3410..4333)
                     /gene="murQ"
                     /locus_tag="VN041_10180"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWU29442.1"
                     /translation="MSDRDQLRTTLETLSTERVDPAFAQLDLLGTSDQVALMAEQGVV
                     AAQAVASERESIARAVDGIVARLHRGGRLVYIGAGTAGRMGVLDASEIPPTFGTDPSL
                     VTGLIAGGEHALRNAVENAEDDIDAGEQAGRELGPDDAIVGISASGRTPYVVGALRAA
                     REQGAFTVSLACNRDSEISKHADAPIEVVVGPEVVAGSTRLKAGTAQKLVINTLSTLA
                     MVQSGKVYGNLMVDVQSTNEKLRARAERTVMHATGVTPEAAAAALASAGGSVKLAIGI
                     VETGVDAETMRRALDAAGGNLRAALAAASAE"
     gene            complement(4378..>4696)
                     /locus_tag="VN041_10185"
     CDS             complement(4378..>4696)
                     /locus_tag="VN041_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HWU29443.1"
                     /translation="SAASHVAQALAVGGETFRIDAEHAADADIDVALRSAGTGQSVVL
                     VDRIDVGSPQRVALDRIRDAIPGVVTVNVGLPVADAGTVITASAASLLAARAARRTLL
                     VSR"
BASE COUNT          659 a         1611 c         1720 g          706 t
ORIGIN      
        1 ccgatctgag cccgggcctg gacggcgtgc ccgcaccgga tgcgtcggac gccgagtggg
       61 tgcgccccga actcgtcggc gaggtcgagt tcgcgaactg gtcgcccagc ggcatcctgc
      121 ggcactcgcg ctggcgcggc ctgcgcccgg acaagtcccc cgaagaggtg cgccgggagt
      181 cctgatcagg cgtgcgtggc ctcgcagtcg tcgtgcccgg cgggctcgag ctggaacgtg
      241 gagtgctcca catcgaagtg gtcggccagg caggactgca gccgggtgag cagctctgcg
      301 gaacggccct gcgcgtagac ggactgggcc acgctcacgt gcgcggtgaa caccggggcg
      361 ccgcgggtga gctgccacac gtgcacgtcg tgcacgtcga cgactccctc gtatcccttc
      421 agatgcgtgc ggatgtcgtc gaccgacatg cccttgggtg cggattcggc gagcaccgag
      481 aagacctcgc gcagcagcga gatcgcgcgg ggcacgatca tcgcggcgat cacgagggag
      541 gcgatcgcgt cggccggcat ccagccggtg gtgacgatca cgatcgccgc gatgatgacc
      601 atcaccgaac cgatcaggtc gcccatgacc tccaggtacg cgccgcggac gttgatgctc
      661 ttctcctgcg cgcggctgag cagccacatc gaggtgccgt tggccagcat gccgacgacg
      721 gcgacggcga gcatgacggg acccgccacc tccacctcgc cggggttgag caggcggttc
      781 acgccgccga tggcgacgct cacggtcagc acgatgagga tgatggcgtt gatgagcgcg
      841 gcgaacacct cggcgcgctg gtatccgaag gtgcgccggt catccgcggg ccgcgcggcg
      901 accgccgtcg cgatgagcgc gatcacgagc gccgaggagt ccgtgaacat atgcgcggcg
      961 tcggccagca gggcgagcga gcccgacagg atcgccccga cgacctgcac caccatgatg
     1021 gtggcggtca ggccgagcga gacggcgagc aggcgccggt gaccggcgtc gcggatgtcg
     1081 cccggggcgg gtgcgtgatc gtgcatgcct ccaggctagg ccgggaagac gccggtcagc
     1141 gccgattccg cctagtcggg aatggggatg attctcaata gcccctagag ctcggcgagc
     1201 agcgggatca gcgcctggaa cgcgcgggcg cggtgcgact gggactgctt ctcggcatcc
     1261 gtgaactcgc cgacggtgcg ctcggatgcg gcatcctggc cgtccgggat gaagatcggg
     1321 tcgtagccga agccgccggg gcccgcgggg ctgtgcgcca ggcggccggg ccagatcccc
     1381 tgcaccgtgc gctccgtgcc atcggcggtc accagcgcga tcgtcgagtg gaactgcgct
     1441 gcgcgatgcg ggtcggcgat gtcgcgcagc tggtccagca gcagctcgag gttggccgtg
     1501 gcatccttct tctgccccgc ccagtatgcc gagaacacgc ccggtgaacc gccgagaaca
     1561 tcgacgcaga tgccggagtc gtcggcgagc gccggcagtc cggtgtgcgc ggcggccgcg
     1621 cgcgccttga tcagggcgtt ctccgcgaac gtcacgccgt cctcgaccgg ctcggggccg
     1681 tcgtagccga tgacctcgag atcggggcgg gtggcggcga cgatctgctg gaactcggcg
     1741 accttgtggg ggttgtgcgt ggcgaggacg accttcatcg cgccgccgcc agcgatgcca
     1801 gctggtgctc cctgagcgac gcgcagccgt tcacgccgag ctccagcagg gcgtcgagct
     1861 cgcgcttgtc gaagggcgcg ccctcggcgg tgccctgcac ctcgacgaac agtccgcggc
     1921 cggtgacgac cacgttcatg tcggtctccg ctcgcacgtc ctcgacgtat gccagatcga
     1981 gcatgggctc gccgtcgatg atcccgaccg agaccgcggc cacggagtcc agcagcggcg
     2041 tggagttctt gccgatgaac ttcttctcgc gaccccactc gatggcgtcg gcgagggcga
     2101 cgtaggcgcc ggtgatcgcg gcggtgcggg tgccgccgtc ggcctggagg acgtcgcagt
     2161 cgatcacgat cgtgttctcg ccgagcgcct tggtgtcgac gacggcacgc agcgcgcggc
     2221 cgatcaggcg ggagatctcg tgcgtgcgac cgccgatgcg gcccttgacg ctctcgcggt
     2281 cgttgcgcga gttggtggcc cgcggcagca tcgcgtactc ggcggtgacc cagcccttgc
     2341 ccttgcccgt cagccagcgc ggcacgccgt tcgtgaacga tgccgtgcac agcaccttgg
     2401 tgccgccgaa gctgacgagc gccgagccct cggcgtgcgc ggaccagccg cgctcgatcg
     2461 tcacctctcg gaactggtcg gtgctgcggc cgtcggcgcg gacgatgtct gacatggtgt
     2521 cctctcggaa ggggttcggg agttcagggc cgcagcgcgt cggcgccgtc gacaccggac
     2581 aggtccggca gagtgatcac gccggtctgc acgagctgca cgtcgcgcac ctcacggccc
     2641 atgagccggt tggcgagcac cgtgaagtcc tcggtcgagg cgccggtcgc ctcgtacgtg
     2701 tacgtcggga cggcgtccgc gggggcgagc tgatcgtgtc gcaccagctg ccggtagaca
     2761 tcggccgcgg tctcgtcgtc gctggagacc agcgagaccc cgtcccccat cacgtagctg
     2821 atcgcgccgc gcaggaacgg gtagtgcgtg cagcccagca cgagcgtgtc gacctcggca
     2881 tcccgcagcg gcgccaggta gccctccgcg acggcgagca cctccggggt tccggtgatg
     2941 ccggcctcca cgaactcgac gaagcgcggg caggcggctg tgaacacctc gagccgctcg
     3001 ttgacctcga gcatgtcctg gtaggcgcgc gagccgatcg tcccgaccgt gccgatcacg
     3061 ccgatacggc cgttgcgggt ggtcgagacc gcgcgtcgca cggccgggcc gatcacctcg
     3121 acgacgggca cgtcgtaccg ctcccgcgcg tcgcggagca tggctgcgga tgccgtgttg
     3181 caggcgatca cgagcatctt cacgccctgg tccacgagcg tgtcgaggac ctcgaggctg
     3241 tatcggcgga cgtccgcgat cggcttcggc ccgtacggcg agtgcgccgt gtcgccgatg
     3301 tagacgagcg actcctgcgg cagctgggca cggatcgccc tggcgaccgt cagcccgccg
     3361 actccggagt cgaagatgcc gatgggcgcg tcgttcatga tccccaagcc tactcggcgg
     3421 atgctgcggc gagcgcggcg cgcaggttcc cgccggcggc gtccagcgcg cggcgcatgg
     3481 tctcggcatc caccccggtc tcgacgatcc cgatcgccag cttgacggag ccgcctgccg
     3541 aagccagcgc cgcagcggcg gcctccgggg tgacgccggt ggcgtgcatg acggtgcgct
     3601 cggcgcgggc acgcagcttc tcgttggtgg actggacgtc gaccatgagg ttgccgtaga
     3661 ccttgccgga ctgcaccatc gcgagcgtgg agagcgtgtt gatgacgagc ttctgcgcgg
     3721 tgcccgcctt caggcgcgtg gagcccgcga ccacctcggg acccacgacg acctcgatgg
     3781 gggcatcggc gtgcttcgag atctcggagt cgcggttgca ggccaggctc accgtgaagg
     3841 cgccctgctc gcgtgcggcg cgcagcgcgc cgacgacgta gggggtgcgc ccggatgccg
     3901 agatcccgac gatcgcgtcg tcggggccga gctcgcgacc cgcctgctcc cccgcgtcga
     3961 tgtcgtcctc ggcgttctcc acggcattgc gcagcgcgtg ctctccccct gcgattaggc
     4021 ccgtcaccag cgaggggtcg gtgccgaacg tgggcgggat ctcgctggca tccagcacgc
     4081 ccatgcgccc cgcggtgccg gcaccgatgt acaccaggcg gccgccgcgg tgcaggcgcg
     4141 cgacgatgcc gtccacggcg cgcgcgatgc tctcccgctc ggacgcgacc gcctgcgcgg
     4201 cgacgacgcc ctgctccgcc atgagggcga cctggtccga ggtgccgagc aggtcgagct
     4261 gggcgaacgc gggatcgacg cgctccgtcg acagcgtctc gagggtggtt cgcagctgat
     4321 cgcggtcgga catgtcgtcc ttcttcctgt tcggatctgt gatggggcag ctcgggctca
     4381 gcgggagacg agcagcgttc gtcgtgcggc tcgcgcggcc agcaggctcg ctgcggatgc
     4441 cgtgatgacg gttcccgcgt ccgcgacggg gagcccgacg ttgacggtca cgacgcccgg
     4501 gatcgcgtcg cggatgcggt cgagcgcgac ccgctgcgga gaccccacgt cgatgcggtc
     4561 gacgagcacg acgctctgtc cggtgcctgc cgagcgcaga gccacgtcga tgtcggcatc
     4621 cgcggcgtgc tcggcgtcga tccggaaggt ctcgccgccg acggcgagcg cctgggcgac
     4681 gtgcgaggcg gcgctg
//
LOCUS       DATMSX010000329        11470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166056,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000329 DATMSX010000000
VERSION     DATMSX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166056"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VN041_10190"
     CDS             complement(<1..507)
                     /locus_tag="VN041_10190"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HWU29444.1"
                     /translation="MNRRARRIPHTAESVAFPLGGIGTGNVSLGARGELRDWEFENLP
                     DKGRLNPRSFFAIHAAPQGGPSVTRVLEARSSGRHDRDAGYGFDELAGLPRLDSAGLH
                     GEYPVVDIDFTDATLPVTVSLHAFTPLVPLDADASGIPAAVLRYRVANPGDAPVTVTV
                     AGSMSHTAG"
     gene            complement(504..2027)
                     /locus_tag="VN041_10195"
     CDS             complement(504..2027)
                     /locus_tag="VN041_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWU29445.1"
                     /translation="MRCTLGVDIGTSSSKGVLAAEDGSILATATRAHDVDRPRTGLVE
                     MDGRVWWDEFVAISRELLASVPDAEVVGVGVSGMGPCILLADENDEPVRPAILYGVDT
                     RSTAQIARLTTELGEDEITRVGGSSLTSQAGGAKIVWVAEEEPDAWARAKRLFMPASW
                     LAHKLTGAYVLDHQSASQVSPFYDVEGERWHAPWWDAYADRIEAPPLTWAGDIAGAVT
                     AEASALTGIPEGTPVITGTIDAWTEAVSAGAHGIGDLMLMYGTTMFLVATVAETLRTP
                     SMWTTAGAFEGTRNLAGGMSTSGALTAWLKDLTDADYPRLLAEAADSGVGARGLLLLP
                     YFAGERTPIQDPDARGVIAGLTLRHGRGDLYRAALEATALGVRHNVETMREAGADIRR
                     IVAVGGGTQGRLWLQIVSDATGLVQEVPATTIGASYGAAFLAATAVAPVGAAPRIEDW
                     NPVVDRIEPDPARRAAYDELFDRYLRLYAGTAEVVHELAESQRDAAHRDAAHREGER"
     gene            complement(2162..3139)
                     /locus_tag="VN041_10200"
     CDS             complement(2162..3139)
                     /locus_tag="VN041_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29446.1"
                     /translation="MATIYDVAQLAGVSPATVSRVFNGVGVSDAKTTAVRHAAEQLNF
                     TPNRAARTLRRQSSEVIALVIPDIENPYFTEMARGVEDVASEAGYSVVLCNSDSQVDK
                     ESTYLRIAMAENMSGVILAAASDHTSLDEILATGRPVVAVDRSTGYDIDGVVMANRDA
                     GRMATEHLLKTHQRVAYIGGPEHIETSSDRAEGWRVAHRAAGIEPPVDLLTFSSFRVD
                     GGRDAMERLLALPEPPDAVVAGNNLIGVGAIQVLTEHALTPPAIGVAVIGGLPFTTLS
                     PSAVTVVRLPARHMGVTAARMLLERIRGDQQPARTVVLRNEVRAARASR"
     gene            3258..4370
                     /locus_tag="VN041_10205"
     CDS             3258..4370
                     /locus_tag="VN041_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HWU29447.1"
                     /translation="MPDLNSIAALRQVQTRSISPENFDGAAGGGGRATEGTGADCARD
                     LGPSWKISPSVDIKAGETFELASIEGAGKITHIWITTHTDNWRTLILRAFWDGADEPA
                     VEVPYGDFFCNGWGVFAQVNSQAIAANPHGGFNSYWPMPFRDGARLTIENTSVVDVRV
                     YYQVTYEIGGDHSNDAYFHAQWRRSNPLEELTPHVILEGIEGEGHYVGTYIAWGVNSN
                     GWWGEGEIKFYLDDDTDHPTICGTGTEDYFGGAWNFDIPGKGYTEFSTPYLGMPQVIR
                     PDGLYVSQQRFGMYRWHLQDPIHFATGIPKVDIQALGWRSGWRYLPLRDDIASTAMFY
                     LDRPTARRPKSPSADDMEVHLGTAPVPDLGATPPRV"
     gene            4373..5119
                     /locus_tag="VN041_10210"
     CDS             4373..5119
                     /locus_tag="VN041_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU29448.1"
                     /translation="MTHATYPSLSGRLVVVTGGGRGIGLQTVRRFLEQGARVVVLDLA
                     MTADDVDADFVKVDVTDPDAVTAAASDIADRFGTPDVVLNAAGIVRSASAEDMSADDW
                     RQVVDIDLSGTFYVCQAFGRLMLEAGRGSIVNIASMSGVISNFPQKQSSYNAAKAGVI
                     HLTKTLAGEWAARGVRVNSVSPGYIGTDLTGALLEREPELGVVWRERTPMGRLGTPDE
                     VASVILFLASDESSFMTGSDVVADGGYTVW"
     gene            5121..6221
                     /locus_tag="VN041_10215"
     CDS             5121..6221
                     /locus_tag="VN041_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HWU29449.1"
                     /translation="MNRRPASIAILAVSAVLLAGCSAASAPIEAVTVADAPTAIGVSD
                     GDLWPSCWSDDGALYAANGDGSGFGSTFSDIVMNRITGTPASDDLAGEGLVSGDALGQ
                     VWTDAAGYTRKPTGMLCLGGALYLAVQDLAPDFNAAPNATIARSDDHGRTWNWDHAAP
                     MFDDGAFTTVWFADYGKDAERRPDKDYVYAYGLDGNWRDSFTDVVSDPTELFLARVPV
                     ASVQDLSTWEFYSGEPGASEDTWTHDVAAKKPVLVDDRRTAHGMSVISQGGVTYLPKH
                     DRYLYTSWTEYTFEFYESPQPWGPWTPVLSEDFGTYPWQADRIGGYATTVPSKFVSDD
                     EKTAWVQSNVCPCAPAGVTSYWFGLRQMTLPG"
     gene            6275..7810
                     /locus_tag="VN041_10220"
     CDS             6275..7810
                     /locus_tag="VN041_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HWU29450.1"
                     /translation="MTPLKRIVAGAAAASLATVAFAAAAAPPPAEATMARTPATVVEK
                     LTGPDAPNNTWGRWDVKATDLGIMWDDGAGHVLTAFGDTFGNAWTGPGGGAPPNGNWR
                     SNVLVRSSDGDLADGMLFDWAAQGPQGVAREIIPSKKINGVEITTIPTTGISVGKRQY
                     LGFMSVKQWGPPGVWDTNFAGIAYSDDGGGTWKVSDTRWENADGHDPFQMQAWVQKGG
                     TIYVFGTQNGRNGPASVAKVPASKLLDKSAFRYWDGADWSRKESDAVPVTDAPMSEMS
                     VQYDAYSKRFLMMTLSGEDIIMRTATAPEGPWSPAQTVASSTDYPALYGGYFHPWNKD
                     GEIYFTMSQWNPYNVYLMRLRIDRDGNIIDPNLVTDASFERSTTLGDGTNGTWAAKPN
                     SGIDNAPAAGFTGDHRAFVRYNSGWRDIWQDVPVERGAKYRLTGFLRTSVNSDNGFFG
                     ARTLDGVPIGEINFHSVGAWTRFTVEFDAGDRDAVQVFGGVWTNSGDIWMQLDDVSLT
                     KVP"
     gene            7899..8444
                     /locus_tag="VN041_10225"
     CDS             7899..8444
                     /locus_tag="VN041_10225"
                     /inference="COORDINATES: protein motif:HMM:NF021872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2441 domain-containing protein"
                     /protein_id="HWU29451.1"
                     /translation="MVTDKPMHVGQQIVFDEVNRSGVWRRVQERAPEVARIYADAASD
                     RPVELDHHLAVAFRELAMEDVRAEGFPDYPSRLSSLYVSETLADAEGWCSLFVDWGRP
                     TYQIVRLTIDGRWFSGDARNCFAGTADRATNLDLARRYWSKAENDGEPILETLVDGRI
                     EVVEIIRTVNANLPPSASEEQ"
     gene            8447..8992
                     /locus_tag="VN041_10230"
     CDS             8447..8992
                     /locus_tag="VN041_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29452.1"
                     /translation="MTDSKRTRGLILNGASGVGKSRTLQELGALLGEKAVAYALVDLD
                     FLTLSWPRPQDDPWGGQVARENLAAVTSNYRRSGIERVVVAHVFTDRTHLDGCRAALG
                     AASADEAPLIRLRASRAVVEQRLRARHAADAPWELDGFLAGHDALTHALDAADLDDHV
                     IDVDGLDPRAVAERVIEAAGW"
     gene            8997..9500
                     /locus_tag="VN041_10235"
     CDS             8997..9500
                     /locus_tag="VN041_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29453.1"
                     /translation="MTDISLADAEPADITGLADAFRDWPKPAALFEHYLDERRAGRID
                     VLVARRDESPAGYCLIIWESAYPPFREVGIPEISDLNVLHAHRGAGLGRLLLDEAERR
                     IATRAGTAGLRVGLYADYGIAQQMYARRGYLPDGRGVSIDGEPVPPGTMIALDDEPVL
                     ALTRALR"
     gene            9563..9946
                     /locus_tag="VN041_10240"
     CDS             9563..9946
                     /locus_tag="VN041_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWU29454.1"
                     /translation="MKYVGGRHPGSADVGTVAEGANCQLYVYAVLDLFGRRPRPHRSS
                     ELWADPSYDHPQRDAAEPLNLVLFNDTDTAWGAHVALQMGPDELLHLCSSEKTPTVWS
                     FERFAAEPRYRRLIGVVRIPGSAPS"
     gene            complement(9932..10777)
                     /locus_tag="VN041_10245"
     CDS             complement(9932..10777)
                     /locus_tag="VN041_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWU29455.1"
                     /translation="MTSPRADRPRAEHRITTRNARFQQWQALQTNRNKRTRAREFLIQ
                     GVRPISLAVEHGWTIRVLIHDAHRELSTWARDLLRTVSAERVGMASVLLAELGEKDEA
                     PELVAVVEMPDDDLGRIPVPADFLGVLFDRPTSPGNVGSIIRSADALGAHGVIVTGHA
                     ADVYDTKSVRSSTGSLFSLPAVRVPSPGEVMAWVGAQRAQGIPLMLVGTDEHGDSDVF
                     DFDFTRPTLLLIGNETKGLSTAWREHCDHMVSIPMAGSASSLNAANAATVVLYEALRQ
                     RSGRG"
     gene            complement(10787..>11470)
                     /locus_tag="VN041_10250"
     CDS             complement(10787..>11470)
                     /locus_tag="VN041_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29456.1"
                     /translation="NLCDGAGQSGRWRPGRVRCRWQPGVGEHPVDRPTAGRATVGGVD
                     ASAIPLAHRRRHVLLVAQESHIFAATVIENVRLGRTDASDADVARALARIGADWVDEL
                     EDGWDTKVGSGGHPLSGAQEQQLALARVLIADPHTIILDEATSAMDPGIARGLEAALA
                     ATVEGRTVIAIAHRLYTAYDADRIAVVDDGRIVELGPHRELVEAGGPYARLWELWRDE
                     GTGSRPPSP"
BASE COUNT         1844 a         3910 c         3988 g         1728 t
ORIGIN      
        1 gccggcggtg tggctcatgc tgccggccac ggtgacggtg acgggtgcgt caccgggatt
       61 ggcgacccgg tagcgcagca cggcagcggg gatgccggag gcgtcggcgt cgagcggcac
      121 gagcggcgtg aacgcgtgca gcgacaccgt cacggggagc gtcgcgtcag tgaagtcgat
      181 gtcgacgacg ggatactcgc cgtgcaggcc tgcggagtcg agccgcggaa ggccggcgag
      241 ctcgtcgaac ccgtagcccg catcgcggtc atgacgtccg ctgctgcgtg cctcgagcac
      301 gcgggtgacc gaggggccgc cctgcggggc ggcgtggatc gcgaagaagg atcgagggtt
      361 gaggcgtccc ttgtcgggca ggttctcgaa ctcccagtcg cgcagctcgc cgcgtgcgcc
      421 gagcgacacg ttgcccgtgc cgattccacc gagcgggaac gccacgctct cggcggtgtg
      481 cgggatccgg cgggcgcggc ggttcatcgc tcgccctccc ggtgggccgc gtcccggtgg
      541 gccgcgtccc gctgcgattc cgcgagctcg tgcacgacct cggcagtgcc cgcgtagagg
      601 cgcaggtagc ggtcgaacag ttcgtcgtag gctgcacgac gggccgggtc cggctcgatg
      661 cgatccacga cggggttcca gtcctcgatg cgcggcgcgg caccgacggg cgcgacagcg
      721 gttgcagcga ggaacgcggc gccgtagctc gcgccgatcg tcgtggccgg cacctcctgc
      781 acgagcccgg tggcgtccga gacgatctgc agccagaggc gcccctgcgt gcctccgccg
      841 acggcgacga tgcggcggat gtccgccccc gcctcgcgca tggtctccac gttgtgccgc
      901 acgcccagtg ccgtggcctc gagagcggcg cggtacaggt cgccacgccc atgacggagg
      961 gtgagcccgg cgatcacgcc gcgggcatcg ggatcctgaa tgggagtgcg ctcgcccgcg
     1021 aagtacggca ggagcagcag gccccgcgcg ccgacgccgg agtcggccgc ctcggcgagc
     1081 agccgcgggt agtcggcatc cgtcaagtcc ttgagccagg ccgtgagcgc acccgacgtc
     1141 gacatgccgc cggcgaggtt ccgggtgccc tcgaaggccc cggcggtcgt ccacatcgag
     1201 ggcgtgcgca gcgtctcggc gacggtcgcc acgaggaaca tcgtggtgcc gtacatcagc
     1261 atcaggtcgc cgatgccgtg cgctccggcg ctgaccgcct cggtccacgc gtcgatcgtg
     1321 cctgtgatca ctggcgtccc ctcggggatg ccggtgaggg cggatgcctc ggccgtgacc
     1381 gcacccgcga tgtcccccgc ccaggtcagc gggggtgcct cgatccggtc ggcgtaggca
     1441 tcccaccagg gcgcgtgcca gcgctcgccc tccacgtcgt agaaggggga gacctggctg
     1501 gcggactggt ggtcgagcac gtaggcgccg gtcagcttgt gcgcgagcca ggaggcgggc
     1561 atgaacagcc gcttggcccg agcccaggca tccggctcct cctccgcgac ccagacgatc
     1621 ttcgcgccgc cggcctgcga ggtgagcgac gagcctccga cacgggtgat ctcgtcctcg
     1681 ccgagctcgg tggtgaggcg ggcgatctgc gcggtggagc gcgtgtccac gccgtagagg
     1741 atggcggggc gcaccggctc gtcgttctcg tcggcgagca ggatgcacgg gcccatcccg
     1801 ctgacgccga cgcccacgac ctcggcatca gggaccgagg cgaggagctc ccgcgagatc
     1861 gcgacgaact cgtcccacca gactcggccg tccatctcca ccaagccggt gcggggccga
     1921 tcgacatcgt gcgcacgcgt cgcggtggcg aggatcgacc cgtcctcggc tgccagcacg
     1981 cccttgctgc tggatgtgcc gatgtcgacc ccgagggtgc agcgcatgtg gtcctcctcg
     2041 gccgatgctg tgttgtcccg cgacctcagc gtacgcgaaa tcgatttcat gacgcaaccg
     2101 gatacatgga cgcgtcacag gggaacgggc cgcgaagacg gcgcttcggc gcccccgagc
     2161 gtcagcgcga agctctggcg gcgcgcacct cgttgcgcag cacgacggtg cgcgcgggct
     2221 gctgatcgcc gcggatgcgc tcgagcagca tgcgtgcggc ggtgacgccc atgtgccgcg
     2281 caggcaggcg caccacggtg accgcgctgg gcgaaagcgt ggtgaacgga agaccgccga
     2341 tcacggcgac gccgatcgcg ggcggtgtca gcgcatgctc ggtgagaacc tggatcgcac
     2401 ccaccccgat caggttgttg cccgccacga ccgcatccgg cggctcgggc agggcgagca
     2461 gtcgctccat ggcatcgcga ccgccgtcga cgcggaacga cgagaacgtg agcagatcga
     2521 cgggcggctc gatccccgcc gcgcgatgcg cgacccgcca gccttcggcg cggtcgctcg
     2581 acgtctcgat gtgctcgggg ccgccgatgt acgccacacg ctgatgggtc ttgaggagat
     2641 gctcggtcgc catgcgcccg gcatcgcggt tcgccatcac gacgccgtcg atgtcgtagc
     2701 cggtgctgcg gtccaccgcc acgacaggcc tgcctgtggc gaggatctcg tcgagactcg
     2761 tgtgatcgga tgccgcggcg aggatcacgc ccgacatgtt ctcggccatg gcgatgcgca
     2821 gataggtcga ctccttgtcg acctgggagt cgctgttgca gagcacgacc gagtagccgg
     2881 cctcgctggc gacgtcctcc actccgcggg ccatctcggt gaagtagggg ttctcgatgt
     2941 ccgggatcac cagggcgatc acctcggagc tctgccggcg cagcgtgcgg gcggcgcggt
     3001 tcggcgtgaa gttcagctgc tcggccgcat ggcgcaccgc cgtcgtcttc gcgtcggaga
     3061 ctccgacgcc gttgaacacg cgcgacaccg tggccggcga aacccccgcc agctgcgcga
     3121 cgtcatagat cgtggccacg tgtctcctcg catgaaaccc tgtgacgaca gggtagcgcg
     3181 ttgaaatcga ttgcaacaga cgcggcgaat aggtactgtg acggaacacc cacacgccga
     3241 cacgaggaag tgcacgcatg cccgatctca actccatcgc cgccctgcga caggtgcaga
     3301 cacgttcgat ctcaccggag aacttcgacg gcgcggccgg cggcggcggg cgcgccaccg
     3361 aagggaccgg cgccgactgc gcccgcgacc tcggtcccag ctggaagatc tcgcccagtg
     3421 tcgacatcaa ggcgggcgag accttcgagc tcgcctccat cgagggcgcc gggaagatca
     3481 cgcacatctg gatcaccacg cacaccgaca actggcgcac cctgatcctg cgggcgttct
     3541 gggacggcgc ggacgagccc gcggtcgagg tgccgtacgg cgacttcttc tgcaacggct
     3601 ggggtgtctt cgcacaggtg aactcgcagg ccatcgccgc gaacccgcac ggcggcttca
     3661 actcctactg gccgatgccg ttccgcgacg gggcgcgcct cacgatcgag aacacctcgg
     3721 tcgtcgacgt gcgggtctac taccaggtga cctacgagat cggcggcgat cactcgaacg
     3781 acgcctactt ccacgcgcag tggcggcgct ccaacccgct ggaggagctg acgccgcatg
     3841 tgatcctgga gggcatcgag ggcgaggggc actacgtcgg cacctacatc gcctggggcg
     3901 tgaactcgaa cgggtggtgg ggcgagggcg agatcaagtt ctacctcgac gacgacaccg
     3961 accacccgac gatctgcggc accggcaccg aggactactt cggcggcgcc tggaacttcg
     4021 acatcccggg caagggctac accgagttct ccacgcccta cctcggcatg ccgcaggtga
     4081 tccgcccgga cggactgtac gtgtcgcagc agcgcttcgg catgtaccgc tggcacctgc
     4141 aggacccgat ccacttcgcc acgggcatcc cgaaggtcga catccaggcg ctcggctggc
     4201 gcagcggatg gcgttacctc ccgctgcgcg acgacatcgc gtcgaccgcg atgttctatc
     4261 tcgaccgccc gaccgcgcgc cgcccgaaga gcccgtccgc ggacgacatg gaggtccacc
     4321 taggaacggc cccggtaccc gacctcggcg cgactcctcc gcgggtgtga ccatgacgca
     4381 tgcgacctac ccctcactgt ccggccggct cgtcgtcgtc accggcggcg ggcgcggcat
     4441 cggactgcag accgtgcgcc ggttcctgga gcagggtgcc cgcgtggtgg tgctcgacct
     4501 cgcgatgacc gcggacgatg tggatgcgga cttcgtgaag gtggatgtga cggatcccga
     4561 cgccgtgacg gcggcggcat ccgacatcgc cgatcgcttc ggcaccccgg atgtcgtgct
     4621 caacgccgca ggcatcgtgc gctctgcgag cgccgaggac atgtcagccg acgattggcg
     4681 gcaggtggtg gacatcgacc tgtccggcac gttctacgtg tgccaggcgt tcggcaggct
     4741 catgctcgag gcgggacgcg ggtcgatcgt gaacatcgcg tcgatgagcg gcgtcatcag
     4801 caacttcccg cagaagcagt ccagttacaa cgcggcgaag gccggcgtca tccacctgac
     4861 gaagaccctc gcgggcgagt gggccgcccg cggcgtgcgg gtgaactcgg tctcgcccgg
     4921 ctacatcgga acggatctga ccggagcgct gctggagcgc gagcccgagc tgggcgtcgt
     4981 ctggcgggag cggacgccga tgggaagact cggcacccct gatgaggtcg cctcggtgat
     5041 cctgttcctc gcctccgacg agtcgagctt catgacggga tccgacgtgg tcgccgacgg
     5101 cgggtacacg gtctggtgac atgaaccgcc ggccggcttc gatcgcgatc ctcgcagtga
     5161 gcgccgtgct gctggccgga tgctccgcgg catccgctcc gatcgaggcc gtgaccgtcg
     5221 cggatgcacc gaccgcgatc ggcgtctcgg acggcgatct gtggccgagc tgctggtcgg
     5281 atgacggtgc actgtatgcc gcgaacggtg acggctcggg gttcggcagc acgttcagcg
     5341 acatcgtgat gaaccggatc acgggaaccc cggcatccga cgatctggcg ggcgaagggc
     5401 tcgtgtccgg cgacgccctc ggccaggtct ggacggatgc cgcggggtac acgcgcaagc
     5461 cgaccgggat gctgtgcctg ggcggcgccc tgtatctggc cgtccaggat ctcgcacccg
     5521 acttcaacgc ggcgccgaac gcgacgatcg cccgcagcga cgaccacggc aggacgtgga
     5581 actgggacca cgcggcgccg atgttcgacg acggcgcctt caccaccgtc tggttcgccg
     5641 actacgggaa ggatgccgag cgccgtcccg acaaggacta cgtctacgcg tacggactcg
     5701 acggcaactg gcgcgactcc ttcaccgacg tcgtgtctga cccgaccgag ctgttcctcg
     5761 cccgtgtccc cgtcgcctcg gtgcaggacc tctcgacgtg ggagttctac tcgggtgagc
     5821 cgggcgcttc cgaggacacc tggacgcacg acgtcgctgc gaagaagccc gtcctggtgg
     5881 acgatcggcg caccgcccac ggaatgagcg tgatctcgca gggcggggtc acgtacctgc
     5941 cgaagcacga ccgctatctc tacacgtcgt ggaccgagta cacgttcgag ttctacgagt
     6001 caccgcagcc ctggggaccg tggacgcccg tgctctccga ggacttcggc acgtacccgt
     6061 ggcaggcgga tcgcatcggc gggtacgcca cgaccgtgcc gtcgaagttc gtctcggatg
     6121 acgagaagac ggcgtgggtg cagtcgaacg tctgcccatg cgctccggcg ggcgtcacgt
     6181 cgtactggtt cgggctcagg cagatgacgc tcccgggctg agcgtccggg tccgctgaga
     6241 cgtcgccgca cggcgacctg agaaaggaga ggcaatgacg cccctgaaga gaatcgtggc
     6301 gggtgccgcc gcggcatccc tcgcgaccgt cgcgttcgcg gcggccgcag cgccaccacc
     6361 ggcggaggcc accatggcgc gcacaccggc gaccgtcgtc gagaagctga ccggacctga
     6421 tgccccgaac aacacctggg gtcgctggga cgtcaaggcg accgacctcg gcatcatgtg
     6481 ggacgacggc gcagggcatg tgctcacggc gttcggcgac acgttcggca acgcgtggac
     6541 gggaccgggc ggcggtgcgc cgcccaacgg caactggcgc agcaacgtgc tggtgcgctc
     6601 cagcgacggc gacctcgccg acgggatgct gttcgactgg gccgcccagg gtccgcaagg
     6661 cgtcgcacgc gagatcatcc cgtcgaagaa gatcaacggt gtcgagatca ccacgatccc
     6721 cacgaccggc atctcggtcg gcaagcggca gtacctcggg ttcatgtccg tgaagcagtg
     6781 gggtccgccg ggcgtgtggg acacgaactt cgccggcatc gcgtactcgg atgacggcgg
     6841 gggcacctgg aaggtgtcgg acacgcggtg ggagaacgcc gacggacacg acccgttcca
     6901 gatgcaggcc tgggtgcaga agggcggcac gatctacgtc ttcggcacgc agaacgggcg
     6961 caacggcccg gcatccgtgg cgaaggtgcc cgcgtcgaag ctgctggaca agtcggcttt
     7021 ccgatactgg gacggcgcgg actggagccg gaaggagtcc gacgccgtgc ctgtgacgga
     7081 tgctccgatg agtgagatgt cggtgcagta cgacgcctac agcaagcggt tcctcatgat
     7141 gacgctgtca ggcgaggaca tcatcatgcg caccgcgacc gccccggagg gcccatggag
     7201 ccccgcgcag accgtggcga gttccaccga ctaccccgcg ctgtacggcg gatacttcca
     7261 cccgtggaac aaggacggcg agatctactt cacgatgtcg cagtggaatc cgtacaacgt
     7321 ctatctgatg cgcctgcgca tcgaccgcga cgggaacatc atcgacccga acctggtcac
     7381 ggatgcgagc ttcgagcgct ccacgacgct cggcgacggc acgaacggca cctgggcggc
     7441 gaaaccgaac tcgggcatcg acaatgctcc ggccgccggc ttcaccgggg accaccgcgc
     7501 gttcgtccgg tacaacagcg gatggcggga catctggcag gacgtacccg tcgaacgcgg
     7561 tgcgaagtac cggctgaccg ggttcctgcg cacctcggtg aacagcgaca acgggttctt
     7621 cggcgcccgg acgctggacg gcgtcccgat cggcgagatc aatttccact ccgtcggcgc
     7681 ctggacccgg ttcaccgtcg agttcgatgc gggcgaccgc gacgccgtgc aggtcttcgg
     7741 cggtgtgtgg accaacagtg gcgacatctg gatgcagctg gacgatgtct cgctgacgaa
     7801 ggtgccctga tcccacggcg gtgggagggt cgcgtggccc tcccaccgcg tccacgacac
     7861 agaaggtgca ggtcatgacg cagaggttcg cgttccacgt ggtgacggac aagccgatgc
     7921 atgtgggtca gcagatcgtg ttcgacgagg tcaatcgaag cggggtctgg cgccgtgtgc
     7981 aggaacgcgc ccctgaggtc gctcggatct acgcggatgc cgcatccgac aggccggttg
     8041 agctcgatca ccatctcgcc gtcgccttcc gagaattggc catggaagac gtgcgagccg
     8101 aggggttccc cgattatccg tcgaggctca gcagcctcta cgtatcggag acgctggcgg
     8161 atgccgaggg atggtgctcg ctcttcgtcg actggggccg cccgacctat cagattgtgc
     8221 gcctcacgat cgacggtcga tggttcagcg gcgacgcgcg caactgcttc gcgggcacag
     8281 cggatcgagc gacgaatctt gacctcgcca gaaggtattg gtcgaaagcc gaaaacgacg
     8341 gcgaaccgat cttggagacg ctcgtcgacg ggaggatcga agtcgtggag atcatccgca
     8401 ccgtgaacgc gaaccttccc ccgtcggcgt cagaggagca ataaggatga ctgactcgaa
     8461 gcgcacgcgg ggcctgatcc tgaacggagc atcgggcgtc ggcaagtcgc gcacactgca
     8521 ggaactcggc gcgctgctcg gcgagaaggc cgtggcatac gcgctcgtcg atctcgactt
     8581 cctgacactg tcgtggcccc gaccgcagga cgatccgtgg ggcggacagg tcgcgcgaga
     8641 gaacctggcc gcggtgacct cgaactaccg gagaagcggc atcgaacgcg tcgtggtggc
     8701 tcacgtgttc acagatagga ctcatctgga cggctgccga gcagctctcg gagcggcatc
     8761 cgccgacgag gcaccgctga tccggctgcg agcctcgcgc gctgtggtcg aacagagact
     8821 ccgcgcccgg cacgccgcgg acgccccgtg ggaactggac gggttcctcg ccgggcacga
     8881 cgccctcacg cacgccctcg atgcggcgga cctcgacgat cacgtcatcg atgtcgatgg
     8941 cctggacccg cgagccgtcg ccgagcgcgt cattgaagcg gccggatggt gacggcatga
     9001 cggacatctc ccttgcggat gcggagcccg cagacatcac cgggctcgct gacgcgttcc
     9061 gcgactggcc caaaccggcc gcgctcttcg agcactacct cgatgagcgg cgagccggcc
     9121 ggatcgacgt gctggtcgcg cgacgggacg agagcccggc cggatactgc ctgatcatct
     9181 gggaatccgc atatccgccc ttccgagaag tgggcatccc agagatctcc gacctcaacg
     9241 tcctgcacgc gcaccgcggt gcagggctcg gtcgcctgct gctcgacgag gcggagaggc
     9301 gcatcgcgac gagagcgggc accgccggcc tccgcgtggg tctgtacgcc gattacggca
     9361 tcgcgcagca gatgtacgcg cgccgggggt atctgcccga tggccgcggc gtcagcatcg
     9421 acggggaacc ggtgccgccg ggcaccatga tcgccctcga cgacgaaccg gtcctcgcac
     9481 tcaccagggc actgagatga cgccgactgc ccctctccac tggcccacgc ccccgggcgg
     9541 gtggcctgag tggctgtggt ccgtgaagta cgtcggtggg cggcatccag gctccgcgga
     9601 tgtcggaacg gtcgccgagg gggccaactg ccagctgtac gtctacgccg tcctcgacct
     9661 cttcggccga cgaccgagac ctcatcggtc cagcgagctg tgggctgacc cgtcctacga
     9721 tcatccgcaa cgggacgccg ccgagccgct gaacctcgtg ctgttcaacg acaccgacac
     9781 cgcctggggc gcgcacgtcg cccttcagat ggggccggac gagcttctcc acctgtgctc
     9841 gtcggagaag acacccacgg tgtggtcctt cgagcgcttc gcggcggaac ctcgatatcg
     9901 acgcctgatc ggggtggtgc ggatccccgg ctcagccccg tcctgacctc tgcctgagcg
     9961 cctcgtagag caccaccgtc gcggcgttcg cggcattcag cgagctggcg gagcctgcca
    10021 tcgggatgct gaccatgtga tcgcagtgct cgcgccaggc cgtgctgagg cccttcgtct
    10081 cgttgccgat cagcagcagc gtggggcggg tgaagtcgaa gtcgaagacg tcgctgtcgc
    10141 cgtgctcgtc ggtgccgacc agcatgagcg ggatgccctg cgcgcgctgc gcccccaccc
    10201 aggccatcac ttcgcccggc gagggcaccc ggactgccgg cagagagaag agcgaacccg
    10261 tgctcgatcg caccgacttg gtgtcgtaca cgtcggcggc atggccggtg acgatcacgc
    10321 cgtgggcgcc gagagcatcc gccgagcgga tgatgctgcc gacgttgccc gggctcgtcg
    10381 gccgatcgaa gagcacaccc aggaagtcgg ccggaaccgg gatgcggccg agatcgtcgt
    10441 caggcatctc caccacggcg acgagctcgg gagcctcgtc cttctcgccg agctcggcga
    10501 gaaggacgga tgccattccc acgcgttccg ctgacaccgt gcgcagcaga tccctggccc
    10561 aggtcgacag ctcgcggtgg gcgtcgtgga tgagcacgcg gatcgtccag ccgtgctcca
    10621 cggcgagcga gatcgggcgg acgccctgga tgaggaactc gcgcgcccgc gtgcgcttgt
    10681 tgcgattcgt ctgcagagcc tgccactgct ggaagcgtgc gttccgtgtg gtgatccggt
    10741 gctccgctcg cggccgatcc gctcgtggcg aggtcatgct gccagcttac ggcgacggcg
    10801 ggcgggatcc cgtcccttcg tcgcgccaca gctcccagag ccgcgcgtac gggccaccgg
    10861 cctccacgag ctcccggtgc gggcccagct cgacgatccg cccgtcgtcg acgacggcga
    10921 tccggtcggc gtcgtacgcc gtgtacagcc gatgcgcgat cgcgatcacg gtacggccct
    10981 ccaccgtcgc ggcgagcgcc gcctccaggc cgcgggcgat gcccggatcc atggcggagg
    11041 tcgcctcgtc gaggatgatg gtgtgcgggt ccgcgatcag cacgcgggcg agcgcgagct
    11101 gctgctcctg cgcgccgctg agcggatgcc cgccggagcc gaccttcgtg tcccagccgt
    11161 cctccagctc atccacccag tcggcgccga tccgcgccag cgccctggcg acgtcggcat
    11221 ccgaggcatc cgtccgtccg agtctgacgt tctcgatcac cgtcgctgcg aagatgtggc
    11281 tctcctgcgc gaccagcagc acgtggcgtc gacggtgggc gagggggatc gccgaggcat
    11341 ccaccccgcc gaccgtcgcc cttccggcag tcggccggtc gacgggatgt tcaccgacac
    11401 ctggctgcca tcggcaccgg acacgcccag gccgccaacg gccagattgc cctgcgccat
    11461 cgcatagatt
//
LOCUS       DATMSX010000330         6843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_156821,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000330 DATMSX010000000
VERSION     DATMSX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156821"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VN041_10255"
     CDS             complement(<1..184)
                     /locus_tag="VN041_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29457.1"
                     /translation="MSAAERLGQARAWLRQDPDAETRDELAGIITRAAAGDTLCRPVP
                     CLARDGELRRIWQSRPR"
     gene            complement(181..1014)
                     /locus_tag="VN041_10260"
     CDS             complement(181..1014)
                     /locus_tag="VN041_10260"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWU29458.1"
                     /translation="MQDTLTHPLDAPDAKPFEVAAQAAEDIARLTGVDHHDIALTLGS
                     GWGKAAELIGETVATIPATEVTGFSKPALEGHVGTLRSIRTSSGKNVLVIGARTHYYE
                     GHGVRRVVHSVRTAAATGAKIMVLTNGAGGVRETWKPGQPVLISDHINLTADSPLEGA
                     TFIDLTDLYAKRLRDIAHSIDPSLDEGVYTQFRGPHYETPAEVQMAKTIGGHIVGMST
                     ALEAIAAREAGMEILGFSLITNLAAGIQKTPLSHAEVIEAGQQAEPVISALLARVIEA
                     L"
     gene            1217..2665
                     /locus_tag="VN041_10265"
     CDS             1217..2665
                     /locus_tag="VN041_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HWU29459.1"
                     /translation="MESMSQTSFANQQSVAVLGGGPGGYEAALAAAQLGADVTLVERV
                     GVGGSAVLTDVVPSKSLIATADAAVAISEASDLGVQFYAKGAHGKPLKPEIAINLAAV
                     NKRLVALAGQQSEDMRATLLEAGVRILSGHGRLEGPNAIVVATGPGGTDFDRIEADTI
                     VVSVGASPRELDSAKPDGERILTWTQLYDMKALPEHLIVVGSGVTGAEFASAYMNLGA
                     EVTLISSRDQVLPGEDGDAAKVLEKVFKRGGMQVLSKSRADRVERTENGVLVTLSDGR
                     TVEGSHCLMAVGSIPNTRGIGLEEAGVELTESGHIRVNRVARTSVSNIYAAGDCTNFF
                     PLASVASMQGRTAVFHALGDIVIPLEQRKITANIFTAPEIATVGFSEQDIENGAADGI
                     AYKLPLAANPRAKMMGIKDGFVKVIARKGSGTVIGAVVVAPKASELIFPLAVAVERRL
                     NVDQVSRVFAAYPSLSSSITDATRAMHMVNMG"
     gene            complement(2662..3144)
                     /locus_tag="VN041_10270"
     CDS             complement(2662..3144)
                     /locus_tag="VN041_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29460.1"
                     /translation="MPEAAASPLSAQELEIWAAVATLLERLPTALDAQLQRDSGLTHF
                     EQGILYALDHSPERRLRLSVLASYASCTLSRMSRAISRLEKQGWVRRETDPTDGRFTL
                     GLLTDAGHAKVVESTPAHHALVRALVFDPLSVSQARQLGALSARISATIDSESSWTPS
                     "
     gene            3250..4041
                     /locus_tag="VN041_10275"
     CDS             3250..4041
                     /locus_tag="VN041_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU29461.1"
                     /translation="MDLGLHGRTAFISGSTQGIGYSIASALAAEGAAVRLNGRDPVRV
                     SAAVDRLRMEHPGSQVSGLVGDVAEPDAVEALLDRLGEADILVNNAGRFGRADFFAID
                     DDEWLRYLEVNLMGGVRLTRRLLPGMLQRGWGRVVFISSESGVNVPADMIHYGVTKAS
                     MVALGSGLAKLTRGTEVTVNTVLGGPTYSEGVAAAVSTVAESRGLPATAVKQAIIEGN
                     RTTLLERFIEPAEIAALVAYLVSPLASATNGAAVRADGGVLTGML"
     gene            complement(4050..5819)
                     /locus_tag="VN041_10280"
     CDS             complement(4050..5819)
                     /locus_tag="VN041_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWU29462.1"
                     /translation="MPAIAKVLIANRGEIAVRIVRAARDSGISSVAVYADQDRDAMHA
                     RLADEAYALDGSTSAETYLQIDKILSVARRSGADAVHPGYGFLAENAEFARAVIAAGM
                     IWIGPSPEAIEALGDKVTARHVAEKVGAPLAPGTPGPVETADEVVAFAEKVGLPIAIK
                     AAYGGGGRGLKVARELGEVAELFESATREAVAAFGRGECFVEKYLDKPRHVETQCLAD
                     AAGNVVVISTRDCSLQRRHQKLVEEAPAPFLTDEQNEQLYAASKAILKEVGYVGAGTC
                     EFLIGADGTVSFLEVNTRLQVEHPVSEEVTGIDLVREQFRIAAGGTIDYDDPTPQGHS
                     FEFRINGEDPGRGFLPQPGPIKVFKTFGGPGVRLDSGVTAGDTVSGAFDSLLAKLIVT
                     GRDRAEALERARRTLDEFEVAGMPTALPFHRKVVRDPAFTAENGEFGVYTRWIETEFV
                     NDIPAWDGELEDPAAAPGRHTVVVEVGGRRLEVSLPDRVTAPVSGTAGRPAAVPPSRR
                     THASTASAGASGDAVKSPMQATIVKVAVEEGQQVVKGDLVVVLEAMKMEQPLQAHKDG
                     VIGNIDAAPGTTVSAGHQLLTIS"
     gene            complement(5890..6546)
                     /locus_tag="VN041_10285"
     CDS             complement(5890..6546)
                     /locus_tag="VN041_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HWU29463.1"
                     /translation="MRVCLASTSPARLMLLRQAGIEPLIFSPEVDEDAVGAAAEAERG
                     APLAPAELVLLLARAKAADVARRLPLEHPDFDGIVIGGDSMFALGGQVYGKPYTPEAA
                     RERWRLMRGATWVLHSGHSVFRVAPGTAPVEATAVAEAGVTFADDLTDAEIDAYIATG
                     EPLLVAGAFTVDSLGGAFITRVDGDPSTVVGMSLSTLRRLAAELGVTWTDLWSASVGS
                     "
     gene            6588..>6843
                     /locus_tag="VN041_10290"
     CDS             6588..>6843
                     /locus_tag="VN041_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWU29464.1"
                     /translation="MTSSQPTLDLDITGIAHGGTFIARHEGRVVFVPDAVPGERVRAQ
                     ITDASKDSFWRAETLEVLDASPHRRAHVWAEADVSRAPEDR"
BASE COUNT         1024 a         2289 c         2494 g         1036 t
ORIGIN      
        1 gtcgcggccg agattgccat attcgacgaa gctcgccgtc gcgagccaga cagggaacag
       61 ggcggcaaag cgtgtcgccg gccgcggcgc gggtgatgat gccggccagc tcatcgcggg
      121 tctcggcatc cggatcctgg cgcagccagg cgcgcgcctg gccgaggcgc tccgcggcgc
      181 tcacagcgcc tcgatcacgc gggcgagcag cgcggagatg acaggctcgg cctgctgtcc
      241 cgcctcgatg acctccgcgt ggctgagagg cgtcttctgg atgccggcgg ccaggttcgt
      301 gatcagggag aagcccagga tctccatccc cgcctcgcgg gcggcgatcg cctcgagcgc
      361 ggtggacatg cccacgatgt ggccgccgat ggtcttggcc atctgcacct cggccggggt
      421 ctcgtagtgc ggaccgcgga actgggtgta gacgccctcg tcgaggctcg ggtcgatgct
      481 gtgtgcgatg tcgcggaggc gcttcgcgta cagatcggtc aggtcgatga aggtcgcgcc
      541 ctccaggggg gagtcggcgg tgaggttgat gtggtcgctg atcagcacgg gctgacccgg
      601 cttccaggtc tcgcgcacgc cgccggcgcc gttcgtcagc accatgatct tcgcgcccgt
      661 ggcggcggcg gtgcgcacgg agtgcaccac gcggcgcacg ccgtgtccct cgtagtagtg
      721 ggtgcgcgcg ccgatgacca gcacgttctt cccggaggag gtgcggatgc tgcgcagcgt
      781 gcccacatgg ccctcgagag ccggcttgga gaacccggtg acctcggtgg cgggaatcgt
      841 cgcgacggtc tcgccgatga gctccgcggc cttgccccag ccggagccga gggtgagcgc
      901 gatgtcgtgg tgatcgactc cggtcagccg cgcgatgtct tctgcggcct gagcggcgac
      961 ctcgaacggc ttcgcgtcgg gcgcgtcgag ggggtgcgtg agagtgtctt gcatgagtcc
     1021 actctaggaa tgtgcggggc gcactgccag tacggaggag tgcccgagtc ccggggcaca
     1081 gccgtcacgg ggttggccgc cggtacggag gacttgcacg agtccgggga cagccgtcac
     1141 ggggttggcc gcctgtacgg aggacttgca cgagtccgag gacagccgtc acggggttgg
     1201 ccgccggtac ggaagaatgg agtccatgtc tcagacttcc ttcgcgaatc agcagagcgt
     1261 ggccgtcctc ggcggcggcc ccggcggata tgaggcagca ctcgccgccg cccagctggg
     1321 cgccgatgtg accctcgtcg agcgggtcgg ggtgggcggt tccgccgtgc tgacggatgt
     1381 cgtcccgtcg aagagcctga tcgccaccgc ggatgccgcc gtcgcgatct cggaggccag
     1441 cgacctgggc gtgcagttct atgcgaaggg tgcccacggc aagccgctca agcccgagat
     1501 cgccatcaac ctcgccgcgg tcaacaagcg cctggtggcg ctggcgggtc agcagtccga
     1561 ggacatgcgc gccacgctgc tcgaggccgg cgtgcgcatc ctctccggtc atggccgcct
     1621 ggagggcccg aacgccatcg tcgtggccac cgggcccggc ggcacggact tcgatcgcat
     1681 cgaggccgac accatcgtcg tctcggtcgg cgcctcgccg cgcgagctcg attccgcgaa
     1741 gcccgacggg gagcgcatcc tcacctggac acagctctac gacatgaagg ctctgcccga
     1801 gcacctgatc gtggtgggct ccggcgtcac gggcgccgag ttcgcctcgg cgtacatgaa
     1861 cctcggtgcc gaggtcacgc tcatctcgag ccgtgaccag gtgctccccg gtgaggacgg
     1921 cgacgccgcg aaggtgctgg agaaggtctt caagcgcggc ggcatgcagg tgctgtcgaa
     1981 gtcgcgcgcc gacagggtgg aacgcaccga gaacggcgtg ctcgtcacgc tctccgacgg
     2041 gcgcacggtg gagggcagcc actgcctgat ggccgtcggg tcgatcccca acacccgggg
     2101 catcgggctg gaggaggccg gagtcgagct gaccgagtcc gggcacatcc gggtcaaccg
     2161 ggtggcgcgc acctcggtgt cgaacatcta cgccgccggc gactgcacga acttcttccc
     2221 gctggcatcc gtcgcctcga tgcagggacg cacggcggtc ttccacgccc tcggcgacat
     2281 cgtcatcccg ctggagcagc gcaagatcac ggcgaacatc ttcaccgcgc ccgagatcgc
     2341 cacggtcggg ttctcggagc aggacatcga gaacggcgcc gccgacggca tcgcctacaa
     2401 gctcccgctg gccgccaacc cccgcgcgaa gatgatgggc atcaaggacg gattcgtgaa
     2461 ggtgatcgcc cgcaagggat cgggcaccgt catcggcgcc gtggtcgtcg cgcccaaggc
     2521 atccgagctg atcttcccgc tggccgtcgc cgtggagcgc cggctgaacg tcgaccaggt
     2581 gtcgcgcgtg ttcgctgcct acccgtcgct gtcctccagc atcacggatg ccacccgcgc
     2641 gatgcacatg gtgaacatgg gctagctcgg cgtccagctg ctctcggagt cgatcgtcgc
     2701 cgagatccgt gcgctgaggg ctccgagctg tcgtgcttgc gagacgctga gcgggtcgaa
     2761 gaccagggcc cgcaccagtg catggtgtgc cggcgtggat tcgacgacct tcgcgtgccc
     2821 ggcgtcggtg agcagcccga gggtgaagcg accgtcggtc gggtcggtct cccgccggac
     2881 ccagccctgc ttctccaagc gcgagatcgc gcgggacatc cgcgacaggg tgcagctcgc
     2941 gtaggaggcg agcacgctca gtcgcaggcg ccgctcgggg gagtgatcca gggcgtacag
     3001 aattccctgt tcgaaatgcg tcagcccgct gtcgcgctgc agctgtgcgt ccagcgccgt
     3061 gggaagcctc tccagaagag tcgcgacagc cgcccagatc tcgagttcct gggcgctcaa
     3121 cggggacgca gcagcttcgg gcatgtcccc agcctacgca gagtcgactt gctcaggcaa
     3181 gtcaactctg cgtaccatca cttgactgag caagtaaatt cgcgcatcga gcgcgagaag
     3241 gagaagcaca tggacctggg gttgcacggc aggacggcat tcatcagcgg ttccacgcag
     3301 gggatcggat actccatcgc ctcggcgctc gccgccgagg gggcggcggt gaggctgaac
     3361 gggcgtgatc ctgttcgcgt atccgccgct gtcgacaggc tgcggatgga gcaccccgga
     3421 tctcaggtct ccgggctcgt cggtgacgtc gcggaaccgg atgcggtcga agcgctgctg
     3481 gacaggctcg gcgaggccga catcctcgtg aacaatgccg ggcgcttcgg ccgggcggac
     3541 ttcttcgcca tcgacgacga tgagtggctg cgctatctgg aggtcaacct gatgggcggc
     3601 gttcggctca cgcgccgcct gctcccgggg atgctgcagc gcggctgggg gcgcgtcgtc
     3661 ttcatcagca gcgagtcagg cgtgaacgta ccggccgaca tgatccacta cggagtcacc
     3721 aaggcgagca tggtggcact gggcagcggg ctggccaaac tcacccgtgg cacagaggtc
     3781 accgtgaaca cggtgctggg cggacccacg tactcggagg gggtcgcggc ggccgtctcc
     3841 acggtggccg agagccgggg gcttccggcc acggcggtca agcaggcgat catcgagggc
     3901 aaccgcacga cgctgctcga gagattcatc gagcccgccg agatcgccgc tctggtcgcc
     3961 tacctcgtca gccccctggc atcggcgacc aacggggcag ccgtccgcgc ggacggaggg
     4021 gtgctcaccg gaatgctctg agcgtgagat cagctgatcg tgagcagctg atgcccggcg
     4081 gagaccgtgg tcccgggggc ggcgtcgatg ttgccgatca cgccgtcctt gtgcgcctgc
     4141 agcggctgct ccatcttcat ggcctcgagc accacgacca ggtcgccctt gacgacctgc
     4201 tggccctcct cgaccgcgac cttcacgatc gtggcctgca tgggcgactt cacggcatcg
     4261 cctgaagcgc ccgcgctggc ggtgctggcg tgcgtccggc gggacggcgg aacggcggcg
     4321 gggcggccgg cggtgccgga gacaggtgcg gtcacgcggt cgggcaggct cacctcgagg
     4381 cggcgtccgc cgacctcgac gacgacggtg tgacgaccgg gcgcggcggc cgggtcctcc
     4441 agctcgccgt cccaggcggg gatgtcgttg acgaactcgg tctcgatcca gcgggtgtac
     4501 acgccgaact cgccgttctc ggcggtgaag gcggggtcgc gcaccacctt gcggtggaac
     4561 ggcagcgcgg tcggcatgcc ggcgacctcg aactcgtcga gcgtgcggcg ggcccgctcc
     4621 agcgcctcgg cgcggtcgcg accggtgacg atcagcttcg ccagcaggga gtcgaacgcg
     4681 ccggagacgg tgtcgcccgc ggtgacgccg gagtccaggc gcacgccggg gccgccgaag
     4741 gtcttgaaca ccttgatggg gccgggctgg ggcaggaagc cccggcccgg gtcctctccg
     4801 ttgatgcgga actcgaagga gtggccctgc ggggtggggt cgtcgtagtc gatggtgccg
     4861 ccggccgcga tgcggaactg ctcgcgcacg aggtcgatgc cggtgacctc ctcggagacg
     4921 gggtgctcga cctgcaggcg ggtgttcacc tcgaggaacg acacggtgcc gtcggcgccg
     4981 atcaggaact cgcaggtgcc ggcgcccacg tagccgacct ccttgaggat cgccttcgag
     5041 gcggcgtaca gctgctcgtt ctgctcatcg gtcaggaacg gtgcgggcgc ctcctcgacg
     5101 agcttctgat gccggcgctg cagcgagcag tcacgggtgg atatcacgac cacgttgccg
     5161 gcggcatccg ccaggcactg cgtctcgacg tggcgcggct tgtcgaggta cttctccacg
     5221 aagcattcgc cgcggccgaa cgccgcgacg gcctcgcggg tggccgactc gaacagctcg
     5281 gcgacctcgc cgagctcgcg ggcgaccttg agaccgcgtc cgccgccgcc gtaggcggcc
     5341 ttgatggcga tgggcaggcc caccttctcg gcgaaggcca cgacctcgtc agcggtctcg
     5401 accggcccgg gggtgccggg ggcgaggggt gcgccgacct tctcggcgac gtgacgcgcg
     5461 gtcaccttgt cgccgagcgc ctcgatcgcc tcgggcgacg ggccgatcca gatcatgccc
     5521 gcggcgataa cagcgcgcgc gaactcggcg ttctcggcca ggaagccgta gccggggtgc
     5581 acggcatccg cgcccgagcg gcgcgcgacg gacaggatct tgtcgatctg caggtaggtc
     5641 tcggcgctcg tcgaaccgtc gagcgcatag gcctcgtcgg cgagacgcgc gtgcatcgcg
     5701 tcgcgatcct ggtcggcgta cacggcgacg gaggagatcc cggagtcgcg ggcggcgcgg
     5761 acgatgcgta cggcgatctc gccgcggttt gcgatgagga ccttggcgat agcaggcatg
     5821 ggtttcagcc tagcgggggt tccgccggtt cttttgacga ctctcgacaa gatatcgagg
     5881 gaactgtcat caggaaccta cggaggcgct ccagaggtct gtccaggtca cgccgagctc
     5941 ggcggcgagg cgtcgcaggg tggacagcga catgccgacc accgtggacg ggtcgccgtc
     6001 cactcgggtg atgaaggcgc cgcccaggct gtcgacggtg aacgcacccg ccaccagcag
     6061 cggttcgccg gtcgcgatgt aggcgtcgat ctcggcatcc gtcaggtcgt cggcgaaggt
     6121 gacgcccgcc tcggcgacgg cggtcgcctc gacgggggcg gtcccggggg cgacgcggaa
     6181 gacggagtgg ccggagtgca gcacccaggt ggcgccgcgc atcagccgcc agcgctcccg
     6241 ggcggcctcc ggcgtgtacg gcttgccgta gacctggccg ccgagcgcga acatcgagtc
     6301 gccgccgatc acgatcccgt cgaagtccgg atgctccagc ggcagccggc gtgcgacgtc
     6361 cgcggccttc gccctggcca gcagcagcac gagctccgcg ggcgccagcg gtgcgccgcg
     6421 ctccgcctcc gccgcggcgc ccacggcatc ctcgtcgacc tcaggagaga agatgagcgg
     6481 ctcgatgccg gcctgccgca gcagcatgag gcgggcgggg gacgtggatg ccaggcagac
     6541 gcgcatgctc ccacgatagg acgctcgcgg tgtgggatcc tggaacgatg acctcctcgc
     6601 agcccacgct cgacctcgac atcaccggaa tcgcgcacgg cggcaccttc atcgcccggc
     6661 acgaggggcg ggtcgtgttc gtccccgacg ccgtccccgg cgagcgggtg cgggctcaga
     6721 tcacggatgc gagcaaggac tccttctggc gcgccgagac gctggaggtg ctcgacgctt
     6781 cgccgcaccg ccgcgcgcac gtgtgggcgg aggccgacgt gtcgcgcgcg cccgaggacc
     6841 ggg
//
LOCUS       DATMSX010000331         7645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_23889,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000331 DATMSX010000000
VERSION     DATMSX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23889"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN041_10295"
     CDS             <1..726
                     /locus_tag="VN041_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HWU29465.1"
                     /translation="GGSDDWRTDAANAAFPLSADGSTTATGTARASTTADGFVDTAER
                     PLWERVSIPQGTLDAASAVYLDMLIRVLEDGLISTGEYSRLDAIAELTQLSPQRLVTL
                     HASYLAAAYVEVLADGIVTADERRELVQIATILDLPVPEIPDVDEPAGDERAFTLAPG
                     ARIVFDGALNRPAEDWARLVTDAGLVTGSVTKSCVVLVAADAASQTGKAKTARAHGIP
                     IVTEAEFEPVFEAYLAQRAGAVS"
     gene            723..1319
                     /locus_tag="VN041_10300"
     CDS             723..1319
                     /locus_tag="VN041_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29466.1"
                     /translation="MNWIDTTASAVEALATVAAVGAAVYAGLYAKRTYEKQADQLEHA
                     KKQAQVEQQQQAQRQAAGLAAWWVRTDDQHRWGIVLANTSATPFFDVRVNATGNFIAS
                     RQPDGGAITMTAVPPGEYFIESLSSEAEKELAATGQVRPCPGWEIGAPMPGATQLKPL
                     LNAYKYRVDQMTFRDNAGRLWEWTPATGTNAQESESMT"
     gene            1386..1724
                     /locus_tag="VN041_10305"
     CDS             1386..1724
                     /locus_tag="VN041_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HWU29467.1"
                     /translation="MFTMTTNDVPGYRITQVLGEVMGLTVRASSFGQNFTAGFRSMGG
                     GEIPEYSRVLYESRMEATNRMWDEAVQRGANAIIAMRYDTSSIQTFFEICAYGTAVVI
                     EPLEPQTSER"
     gene            complement(1737..1976)
                     /locus_tag="VN041_10310"
     CDS             complement(1737..1976)
                     /locus_tag="VN041_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29468.1"
                     /translation="MSPADTDDEFTGIHCRLDELLAERGMTLTELSARVGVSIVNLSV
                     LKNDRARAIRYSTLRAICDALECEVGELLVLAERG"
     gene            complement(1973..2737)
                     /locus_tag="VN041_10315"
     CDS             complement(1973..2737)
                     /locus_tag="VN041_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29469.1"
                     /translation="MQLTSDDLLGGLGWIALWLAIVGVIVAFSIWRARRHGSTTLALD
                     ATRNASLAYLALCAAGIVMGAARYLSSGSLALDDAMQRWVTQQQDSLRTPHCSTDGVF
                     PDDSASHPGTDLMTFCQGWIDNVPFGPRLVIYLGTLLSILASAAIAWAIYTATRRAGL
                     RDPFHPSVSRTFGVASIMVMICGALGTVATSIGMTLAARSLAWAHGVSIPFELSVPLW
                     PFAVAVGLFALSAIFRYGAQLQREKELLQRETDGLV"
     gene            complement(2737..3333)
                     /locus_tag="VN041_10320"
     CDS             complement(2737..3333)
                     /locus_tag="VN041_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HWU29470.1"
                     /translation="MGEPSTPAPAGASLVWTSILAAATVAGIIVVAVIRFTGAFTNAG
                     LSLEVPIPESFGAQHAPSGASLQVTQAIVTVTGADAGLVTLVCGTILLAAIAGVAVVV
                     CYLLLTVEVSRSRTFSRRSIRMLSALSAIILIATTGCYLLDSAIARAVRTAAGLGEVG
                     AITSLSYWMAFAIVGALGLIAYAFRKGAALQRETEGLV"
     gene            complement(3333..3947)
                     /locus_tag="VN041_10325"
     CDS             complement(3333..3947)
                     /locus_tag="VN041_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29471.1"
                     /translation="MNSRSRGMPLAEAITLGLVATGAVSIAIAALTGAVGRAIEIFSS
                     PVTVDMPVHDAAVPALADAAGVASAEFTESTVSFETLDAGVRWLLLGETVLPALATVM
                     VCMTLWWLGFSLIRQQAFRRSMVPVLATAAMSLIVAGMIAPLLGGIARAEAVKALPET
                     ASDMFWTFLVRLDASPIGWGIALALIAAAFEVGRRMQRDTEGLV"
     gene            4286..6808
                     /locus_tag="VN041_10330"
     CDS             4286..6808
                     /locus_tag="VN041_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWU29472.1"
                     /translation="MFERFTDRARRVVVLAQEEAKMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLDAVREQVQDIIGQGQQQPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNKVRQQVIQLLSGAPGGREAATVGAQTNE
                     GPASAQGGSQVLDQFGRNLTQAAREGKLDPVIGREKEAERVMQILSRRSKNNPVLIGE
                     PGVGKTAVVEGLAQAIVKGDVPETLKDKQLYSLDLGSLIAGSRYRGDFEERLKKVTKE
                     IRTRGDIIVFIDEIHTLVGAGAAEGAIDAASILKPLLARGELQTIGATTLDEYRKYFE
                     KDAALERRFQPVQVNEPTLPHTINILKGLRDRYEAHHKVQITDGALVAAANLADRYVS
                     DRFLPDKAIDLIDEAGARLRLSILSSPPELREFDEKIAKVREDKETASEEQDFEKAAA
                     LRDQEKELLGERLRLEKQWKSGDVSTPAVVDEGLIAEVLAQATGIPVFKLTEEESSRL
                     VFMEKALHQRVIGQEEAIAALSRTIRRQRAGLKDPKRPSGSFIFAGPTGVGKTELAKA
                     LAEFLFDDEAALISLDMSEFGEKHTVSRLFGAPPGFVGFEEGGQLTEKVRRKPFSVVL
                     FDEIEKAHPDIFNSLLQILEEGRLTDGQGRVVDFKNTVIIMTTNLGSSAIAGGPVGFQ
                     VEGNSQTTYERMKGKVDEELKRHFKPEFLNRLDDIIVFPQLNKDELRQIVGLFVKQLA
                     DRLLDRDMTVELSDAAKDKLIEIGFDPALGARPLRRAMQREIEDQLSENILHGELNSG
                     DHVKVDVVDGKFTFDAAPRGEKVSVGVSTSPTIPSTPDLAAG"
     gene            7022..>7645
                     /locus_tag="VN041_10335"
     CDS             7022..>7645
                     /locus_tag="VN041_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWU29473.1"
                     /translation="MSAASALIGALAEWQVRHVFCCPGTSEIPVLDAIVELRGEAHVP
                     EFILTTHEAIAVSMADGYARASGDIGVAYLHTNVGVANGLAHLYAAQVARSRVAVLAG
                     IKGTATLPHRALTTSPRLLETAAPYAGHVWQNLRPEDLLDDVSRMLWSTGVVPEQPAV
                     LTVPQNHLMSEAGGAGHVHVRSSARQSPDPDAVREAAELLRHARHPVI"
BASE COUNT         1361 a         2673 c         2526 g         1085 t
ORIGIN      
        1 ggcggctccg acgactggcg gacggatgcc gcgaacgcgg cgttcccgct gtccgccgac
       61 ggatccacga ccgcgacagg gaccgcccgt gcctccacga cagccgacgg gttcgtcgac
      121 acggccgagc gcccgctgtg ggagcgcgtc agcatcccgc agggcacgct ggatgcggca
      181 tccgccgtct acctcgacat gctgatccgc gtgctggagg acggtctgat ctcgaccggc
      241 gagtactcgc gcctcgacgc gatcgcggag ctgacgcagc tcagcccgca gcgcctcgtc
      301 accctgcacg cctcgtacct cgcggccgcc tacgtcgagg tgctcgccga cgggatcgtc
      361 accgccgacg agcgccgcga gctcgtccag atcgcgacga tcctcgacct gccggtgccc
      421 gagatccccg acgtcgatga gcctgccggt gatgagcgcg cgttcaccct cgcgcccgga
      481 gcgcgcatcg tcttcgacgg cgcgctgaat cggcccgctg aggactgggc acggctcgtc
      541 acggatgccg ggctcgtcac cggcagcgtg acgaagagct gcgtggtgct ggtcgcggcg
      601 gatgctgcga gccagacggg caaggccaag accgctcgcg cacacggcat cccgatcgtc
      661 accgaagccg agttcgagcc ggtgttcgag gcgtacctgg cacaacgcgc tggagccgtg
      721 tcgtgaactg gatcgacaca acggcttcag ccgtggaggc tcttgccacg gtcgcagccg
      781 tcggcgccgc ggtgtacgcc gggctatacg ccaaacgcac ctacgagaag caggcggacc
      841 agctcgaaca tgccaagaag caggcgcagg tcgaacagca acagcaggcg cagcggcagg
      901 ccgctggttt ggctgcctgg tgggtgcgga ccgacgatca gcaccgctgg ggcatcgtgc
      961 tcgcgaacac gtcagccacg ccgttctttg atgtgcgcgt gaacgcgaca gggaacttca
     1021 tcgcctcaag gcaacccgat ggaggcgcga tcacgatgac ggccgtgcct ccgggggagt
     1081 acttcatcga atcgctctcg tccgaggcag agaaggagct tgccgctacc ggtcaggtgc
     1141 gaccttgccc gggctgggaa atcggagctc cgatgcccgg cgccacgcag ctgaaaccgc
     1201 tgttgaacgc ttacaaatat cgggtcgacc agatgacttt ccgtgacaac gccggacggc
     1261 tctgggagtg gacaccggcc acgggcacga atgcgcagga gagcgagtcg atgacgtgag
     1321 tcgaacggat cgcgctcagc tcggcaaggc gtgcgagaga cgatgaccta cgccaggatg
     1381 tgcctatgtt cacgatgacg acgaacgacg ttcccggtta ccgcatcacg caggtgctcg
     1441 gcgaggtgat gggtctgacc gtgcgcgcgt ccagcttcgg gcagaacttc accgcagggt
     1501 tccgttcgat gggcgggggc gagattcccg agtacagcag ggtgctctac gagagccgca
     1561 tggaggccac gaaccggatg tgggacgagg ccgtccaacg cggcgccaac gccatcatcg
     1621 ccatgcgcta tgacaccagc tccatccaga ccttcttcga aatctgcgcc tacggcaccg
     1681 ccgtggtcat cgagccgctc gagcctcaga cttcagagcg atagggaaac tcccgctcac
     1741 ccccgctccg cgagaaccag cagctcgccc acctcgcatt ccagcgcgtc gcagatcgcg
     1801 cgcagcgtgg agtagcggat cgcccgcgcg cggtcgttct tcagcacgga caggttcacg
     1861 atgctcacgc cgacccgcgc gctcagctcg gtcagcgtca tcccgcgctc ggcgagcagc
     1921 tcgtcgagcc ggcagtggat gccggtgaac tcgtcgtccg tgtcagcggg gctcatacga
     1981 gaccgtccgt ctctcgctgc agcagctcct tctcgcgttg cagctgcgcg ccgtagcgga
     2041 agatcgccga cagggcgaac agaccgacgg cgacggcgaa gggccagagc gggacgctga
     2101 gctcgaacgg gatgctgacc ccatgcgccc aggcgagcga cctcgcggcc agggtcatgc
     2161 cgatcgaagt cgcgaccgtg cccagtgctc cgcagatcat caccatgatc gacgcgacgc
     2221 cgaacgtgcg ggacacagac gggtggaacg ggtctcgcag gcccgcccga cgcgtcgccg
     2281 tgtagatcgc ccaggcgatc gcggctgagg cgaggatgct cagcagcgtg ccgaggtaga
     2341 tgaccaggcg cgggccgaac ggcacgttgt cgatccagcc ctggcagaac gtcatcagat
     2401 cagtacccgg gtgagatgcg gaatcgtccg ggaagacgcc atcggtgctg cagtgcgggg
     2461 ttcgcaggga atcctgctgc tgcgtcaccc accgctgcat cgcatcgtcg agagccaatg
     2521 atcccgagct caggtatcga gctgcgccca tcacgatccc cgcggcgcag agagccaggt
     2581 aggcgagaga cgcgttgcgc gtcgcatcca gcgcgagcgt ggtgctgccg tgccggcgcg
     2641 cgcgccagat cgagaaggcc acgatcaccc cgacgatcgc gagccacagc gcgatccatc
     2701 ccagaccgcc gagcagatca tccgatgtga gttgcatcag accaatcctt ccgtctctcg
     2761 ctgcagcgcc gcacccttcc ggaacgcata ggcgatcagt ccgagggcgc cgacgatcgc
     2821 gaaggccatc cagtagctca gcgacgtgat cgccccgacc tcgccgaggc cggccgccgt
     2881 gcgcacggca cgggcgatcg cgctgtccag caggtagcat ccggtcgtcg cgatgaggat
     2941 gatcgccgag agcgcgctca gcatccggat cgacctgcgc gagaacgtcc gcgaccggga
     3001 gacctccacg gtcagcagca gatagcagac cacgaccgcg acgcccgcga tcgccgccag
     3061 caggatcgtg ccgcacacga gcgtgacgag cccggcatcc gctccggtca cggtgacgat
     3121 cgcctgcgtc acctgcagcg atgccccgga gggggcgtgc tgtgcgccga agctctccgg
     3181 aatcggcacc tccagggaca gccccgcgtt cgtgaacgct ccggtgaagc ggatcaccgc
     3241 gaccacgatg atgcccgcga cggtcgctgc ggccaggatg ctcgtccaga cgaggctcgc
     3301 gcccgcgggt gcgggggtcg acggctcgcc catcagacca gaccctccgt gtcgcgctgc
     3361 atgcgccggc cgacctcgaa cgcagcggcg atcagcgcga gcgcgatgcc ccagccgatc
     3421 gggctcgcgt cgagtcgcac gaggaacgtc cagaacatgt cggatgccgt ctccggcagc
     3481 gccttcacgg cctcggcccg cgcgatgccg ccgagcagcg gcgcgatcat ccccgcgacg
     3541 atcaggctca tcgccgccgt cgccagcacg ggcaccatcg acctgcggaa cgcctgctga
     3601 cggatcagcg agaacccgag ccaccacagt gtcatgcaga ccatcacggt ggccagcgcc
     3661 ggcagcactg tctcgcccag caggagccag cggacgccag cgtccagcgt ctcgaacgac
     3721 accgtcgact ccgtgaactc ggcggatgcc accccggccg cgtccgcgag cgccggcacc
     3781 gcggcatcgt gcaccggcat gtccaccgtg accggcgagg agaagatctc gatcgccctg
     3841 cccaccgccc ccgtcagcgc cgcgatcgcg atgctcaccg ccccggtcgc tacgagcccg
     3901 agcgtgatcg cctcggccag cggcatcccc ctgctcctgc tgttcatccc ctcaaccctc
     3961 cgtatcgaca atcgttgtta tcgcttttcg ttaacataac gacagtcgat aacaccgtca
     4021 agggtgggca tgcaaactca cgagcgtgag ggcgcagcga agcttccgga gtgagcatcg
     4081 gggaggggcc cgcggagcgg gaggggtccc ccgttctgcg aacgaaggaa gcttcgcgga
     4141 gcccgcccac cggcatcttg aagtcactcg caacagaagg tatgccgacg gcgaacacgg
     4201 ccgtccgacc ccgcgccggt cgttaccctc gatacaaggc cgcagttgaa gctgcgtgcc
     4261 aggcgtagag ccaaggagtc acagcatgtt cgagagattc acggaccgag cccgtcgtgt
     4321 ggtcgtcctc gcccaggaag aggcgaagat gctcaaccac aactacatcg gcaccgagca
     4381 catcctgctc ggcctcatcc acgagggcga gggcgtcgct gcgaaggcgc tcgagagcct
     4441 gggcatctcc ctcgatgcgg tgcgcgagca ggttcaggac atcatcggcc agggccagca
     4501 gcagccgacc ggccacatcc cgttcacccc gcgtgccaag aaggtgctcg agctgtccct
     4561 gcgcgaggcg ctgcagctcg gccacaacta catcggcacc gagcacatcc tgctcggcct
     4621 catccgcgag ggcgagggcg tcgcagccca ggtgctcgtc aagctcggcg ccgacctgaa
     4681 caaggtgcgc cagcaggtca tccagctgct ctccggcgcc cccggcgggc gcgaggccgc
     4741 cacggtcggt gcgcagacga acgagggccc ggcatccgcg cagggcggtt cgcaggtgct
     4801 cgaccagttc ggtcgcaacc tcacccaggc cgcccgcgag ggcaagctcg acccggtgat
     4861 cgggcgcgag aaggaggcgg agcgggtcat gcagatcctc tcgcgccgct ccaagaacaa
     4921 ccccgtcctg atcggcgagc ccggcgtcgg caagaccgcc gtcgtcgagg gcctggccca
     4981 ggccatcgtc aagggcgacg tgcccgagac gctcaaggac aagcagctct actcgctcga
     5041 cctcggctcg ctcatcgccg gttcccgcta ccgcggtgac ttcgaggagc gactgaagaa
     5101 ggtcaccaag gagatccgca cccgcggcga catcatcgtc ttcatcgacg agatccacac
     5161 cctggtgggt gcgggcgccg ctgagggcgc gatcgacgcg gccagcatcc tgaagccgct
     5221 gctcgcccgc ggcgagctgc agaccatcgg tgcgaccact ctcgacgagt accgcaagta
     5281 cttcgagaag gatgccgcgc tcgagcgccg cttccagccg gtgcaggtca acgagcccac
     5341 gctgccgcac acgatcaaca tcctgaaggg tctgcgcgac cgctacgagg cgcaccacaa
     5401 ggtgcagatc accgacggcg cactcgtcgc tgcggcaaac ctcgccgacc ggtacgtcag
     5461 cgaccggttc ctcccggaca aggcgatcga cctgatcgac gaggccggag cccgtctgcg
     5521 cctgagcatc ctgtcctccc cgccggaact gcgcgagttc gacgagaaga tcgcgaaggt
     5581 ccgcgaggac aaggagaccg cctccgagga gcaggacttc gagaaggctg cggccctgcg
     5641 cgaccaggag aaggagctgc tcggcgagcg gctgcgcctg gaaaagcagt ggaagagcgg
     5701 cgacgtctcg acccccgcgg tcgtggacga gggcctgatc gccgaggtgc tcgcccaggc
     5761 caccggcatc ccggtgttca agctcaccga ggaggagtcc agccgcctcg tgttcatgga
     5821 gaaggcgctg caccagcgcg tcatcggtca ggaggaggcg atcgccgccc tctcccgcac
     5881 gatccgccgc cagcgcgccg gcctgaagga cccgaagcgt ccctccggct cgttcatctt
     5941 cgccggcccc accggcgtcg gaaagaccga gctcgccaag gcgctcgccg agttcctgtt
     6001 cgacgacgag gcagccctga tctccctcga catgtcggag ttcggcgaga agcacaccgt
     6061 ctcgcgtctg ttcggcgctc ctcccggatt cgtcgggttc gaagagggcg gccagctcac
     6121 cgagaaggtg cgccgcaagc cgttctccgt cgtgctgttc gacgagatcg agaaggccca
     6181 ccccgacatc ttcaactcgc tgctgcagat cctcgaagag ggtcgcctga ccgacggcca
     6241 gggtcgcgtg gtcgacttca agaacaccgt gatcatcatg acgaccaacc tcggctcgtc
     6301 ggcgatcgcc ggtggtccgg tcggcttcca ggtggagggc aactcgcaga ccacctacga
     6361 gaggatgaag ggcaaggtcg acgaggagct caagcgccac ttcaagcccg agttcctgaa
     6421 ccgcctcgac gacatcatcg tcttcccgca gctgaacaag gacgagctcc gccagatcgt
     6481 gggcctgttc gtcaagcagc tggccgaccg cctgctcgac cgtgacatga cggtggagct
     6541 gtcggatgct gcgaaggaca agctcatcga gatcggcttc gacccggcgc tcggtgcccg
     6601 tccgctgcgc cgcgccatgc agcgcgagat cgaggaccag ctgtccgaga acatcctgca
     6661 cggagagctg aacagcggcg accacgtgaa ggtcgacgtg gtcgacggca agttcacctt
     6721 cgacgccgcc ccgcgcggtg agaaggtctc ggtcggcgtc agcaccagcc cgacgatccc
     6781 gtccaccccg gatctggccg caggctgagc ggttcgcacg gacacgaggg cccggatgct
     6841 tcggcatccg ggcccttcgt cgtctccggg ctcagcggac cgcaggctcc gcagcccgtg
     6901 ccgacgcggc gtcgcaaacc ggcatccgct cttcctccgc catgtcgatg tggcctcgtg
     6961 acggctcccg tagcgttcga gtgatcgtcc cggggcaccg tcgcccggga gggagggtga
     7021 catgtccgcg gcatccgcac tcatcggagc actggccgag tggcaggtgc gtcacgtgtt
     7081 ctgctgcccg ggcaccagcg agatccccgt gctggacgcg atcgtcgaac tgcgcggtga
     7141 ggcgcacgtc ccggagttca tcctgaccac gcacgaggcg atcgccgtga gcatggcgga
     7201 cggctacgcc cgcgcctccg gagacatcgg cgtcgcgtac ctgcacacga acgtcggcgt
     7261 cgccaacgga ctggcgcacc tgtacgccgc acaggtcgcg cgcagccggg tcgcggtgct
     7321 cgccgggatc aagggcaccg cgacgctgcc tcatcgggcg ctgaccacct cgccccggct
     7381 gctcgagacg gcggcgccgt acgcggggca cgtctggcag aacctgcgcc cggaggatct
     7441 gctcgacgac gtgtcgcgga tgctgtggag caccggggtc gtgcccgagc agcccgcggt
     7501 gctgaccgtg ccgcagaacc atctgatgtc ggaagccggc ggggcagggc atgtgcacgt
     7561 gcgatcgtcc gcgcgccaga gcccggatcc ggatgccgtg cgcgaggccg ccgagctgct
     7621 gcgccacgcg cggcatccgg tgatc
//
LOCUS       DATMSX010000332         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_99893,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000332 DATMSX010000000
VERSION     DATMSX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99893"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VN041_10340"
     CDS             complement(<1..269)
                     /locus_tag="VN041_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29474.1"
                     /translation="MERGSDAAARRGLVRWPMVCWLVILVLIGVAQLVRGEWFDAAVF
                     LIAIALVCCSILLPSHGAHRPRPDLVVFAATALAAAVALLQRHSTG"
     gene            391..1521
                     /locus_tag="VN041_10345"
     CDS             391..1521
                     /locus_tag="VN041_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU29475.1"
                     /translation="MSLPRDDIDPDGLLEYSVVFTDRSLNHMSARFVGIMQQTLAILR
                     ETYGADSAAIVNGGGSYAMESVARHLATGRKALVVRNGLFSYRWSQILDAGSIASAVT
                     VCQARPESDDPQSAWSPAPIDEVVAVIRAGRPDVVFAPHVETAAGILLPDDYVSALAA
                     AVHEVGGVLVLDCIASGAMWVDMRALGVDVLLSAPQKGWSGSPGVGFVMLSERGRDAV
                     ASGTPTSFALDLNRWLAISDGYRDGRAGYHATMPTDTIARNLAQMIETRDRGLESLRA
                     AQLELGTRVRALLTERGLPSVAASAFAAPSVVVVHTTDEGLRTGAKFREQGLQIAAGV
                     PLHCDEPDSFSTFRMGLFGLDKLGDVDGTVARLEAALDAMRV"
     gene            1603..2448
                     /locus_tag="VN041_10350"
     CDS             1603..2448
                     /locus_tag="VN041_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWU29476.1"
                     /translation="MSEKIVVGVTDAQASRRAVDWAVSRANARGQSIELLGVVGGAIG
                     MVGEGIVIQAATEATDQLLAEVADRIRADGLTVTTRTALGDPVSTLIEASEHASLLVI
                     GSDYRGPGQGPARGSHGIRIAAGSHCPVVVVPDFDLEDGRRGVVVGVDGSPVSEDAIA
                     FAAAEADRLGEPLTAVIVWTPISAPRNALMTTSAGYRQGMEQASREALSLSLAGLRSQ
                     YPGLEIVEAVAEGFPSAVINDLAEEARLTVVGSRGHGVVRRFLLGSISHEVLQRLASV
                     TAVVR"
     gene            complement(2467..>2767)
                     /locus_tag="VN041_10355"
     CDS             complement(2467..>2767)
                     /locus_tag="VN041_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWU29477.1"
                     /translation="RPGVTAHEVDAAARQVLADAGLGEAFLHRTGHGIGVSVHEEPYI
                     APGNDLPLREGMAFSIEPGIYFPGEWGARIEDIVVVTADGCVRLNNAPHALASVG"
BASE COUNT          413 a         1013 c          951 g          390 t
ORIGIN      
        1 ccggtgctgt gccgctgcag cagcgcgacc gccgccgcga gtgccgtcgc ggcgaacacg
       61 acgaggtccg gccgcggccg atgcgccccg tgcgacggaa gcaggatgct gcagcagacg
      121 agcgcgatcg cgatcaggaa caccgcggcg tcgaaccact ccccgcgcac gagctgcgcg
      181 actccgatga gcaccaggat gacgagccag cacaccatcg gccagcgcac gagcccgcgc
      241 cgtgccgcag catccgatcc acgctccatc tcagctccac ccgcatgcta gtcacctcgg
      301 cgacgcggca cgagtccctc tccctcggac gagacttccg ccaggtgaaa gggcctttcc
      361 ggaacgcgcg agcggtgcga acctggacgc atgagcctcc cccgcgacga catcgatccc
      421 gacggcctgc tggagtactc ggtggtcttc accgaccgct ccctgaacca catgtctgct
      481 cgtttcgtcg gcatcatgca gcagacgctc gccatcctgc gcgagaccta cggtgccgac
      541 agcgcggcga tcgtgaacgg cggcggcagc tacgccatgg agtccgtcgc gcgtcacctc
      601 gccaccggca ggaaggcgct cgtcgtccgc aacggactgt tctcctaccg ctggtcgcag
      661 atcctcgacg ccggatccat cgcatccgcc gtcaccgtct gccaggcgcg ccccgagagc
      721 gacgatccgc agtccgcctg gagccccgct ccgatcgacg aggtcgtcgc ggtgatccgc
      781 gccgggcgcc ccgacgtcgt cttcgccccg cacgtcgaga ccgccgcggg catcctgctt
      841 cccgacgact acgtgagcgc gctcgccgcg gccgtgcacg aggtgggcgg cgtgctcgtg
      901 ctggactgca tcgcctccgg cgcgatgtgg gtggacatgc gcgcgctcgg cgtcgacgtg
      961 ctgctgagcg ccccgcagaa gggctggagc ggctcgcccg gagtcggctt cgtcatgctc
     1021 agcgagaggg gacgggatgc tgtggcatcc ggcacgccga ccagcttcgc cctcgacctg
     1081 aaccggtggc tggcgatctc ggacggctac cgcgacggca gggccggcta ccacgcgacc
     1141 atgcccaccg acacgattgc acgcaacctc gcgcagatga tcgagacccg cgaccgcggc
     1201 ctggagagtc tccgtgcggc gcagctcgag ctcggcacgc gcgtgcgtgc cctgctgacc
     1261 gagcgcgggc tgccgtccgt cgcggcatcc gctttcgccg cacccagcgt ggtcgtcgtg
     1321 cacaccaccg acgagggcct gcgcaccggc gcgaagttca gagagcaggg gctgcagatc
     1381 gccgccggcg tgccgctgca ctgcgacgag ccggactcgt tctccacctt ccggatgggt
     1441 ctgttcggcc tcgacaagct cggcgacgtc gacggcaccg tcgcacgtct ggaggcggca
     1501 ctggacgcga tgcgggtctg acgcgttttc cacggccccg gcccgcgcgg aatcgcacgg
     1561 gggtcaatag agtggaggcc cctgcagaga tgggagccgg agatgtcgga gaagatcgtc
     1621 gtcggtgtga ccgacgcaca ggcgagccgt cgagccgtcg actgggcggt gtcgcgcgcc
     1681 aacgcacgcg ggcagtccat cgagctgctg ggcgtcgtcg ggggcgccat cggaatggtc
     1741 ggcgagggca tcgtcatcca agcggcgacc gaagccaccg atcagctgct cgccgaggtc
     1801 gcggaccgga tccgagccga cgggctcacc gtcacgacac ggaccgcgct cggcgacccg
     1861 gtctcgacgc tcatcgaggc atccgagcac gcgagtctgc tggtgatcgg cagcgactac
     1921 cggggtccgg gccaggggcc tgcacgcgga tcgcacggca tccgcatcgc cgccggctcg
     1981 cactgccctg tcgtggtggt gcccgacttc gacctggagg acgggcgccg cggggtggtc
     2041 gtcggagtgg acggatcacc ggtctccgaa gacgcgatcg ccttcgccgc agcggaggcg
     2101 gatcgtctcg gagaaccgct caccgccgtg atcgtctgga ccccgatctc cgctccccgc
     2161 aacgcgctga tgaccacatc cgccggctac cgtcagggga tggagcaggc ctcccgcgag
     2221 gcgctctcgc tgtcgctggc gggccttcgc agccagtacc cgggcctcga gatcgtggag
     2281 gccgtcgccg agggattccc ctccgcggtc atcaacgacc tcgccgagga ggcgcggctc
     2341 acggtcgtcg gcagccgagg ccacggcgtc gtgcgccgct tcctgctcgg atcgatcagc
     2401 cacgaggtgc tgcagcggct cgcatccgtc acggccgtgg tgcgctgacg gcgcagtccg
     2461 cggcgctcag ccgacggatg ccagggcgtg cggcgcgttg ttgagccgca cgcacccgtc
     2521 cgccgtgacc acgacgatgt cctcgatgcg cgcgccccac tcgcccggga agtagatgcc
     2581 aggctcgata ctgaaggcca tgccctcgcg cagcggaagg tcgttgcccg gcgcgatgta
     2641 cggctcctcg tgcaccgaga cgccgatgcc gtgaccggtg cgatgcagga acgcctcacc
     2701 gagcccggca tccgccagca cctgccgtgc cgcagcgtcg acctcgtgcg cggtcacgcc
     2761 ggggcgc
//
LOCUS       DATMSX010000333        11780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_52457,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000333 DATMSX010000000
VERSION     DATMSX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52457"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VN041_10360"
     CDS             complement(<1..112)
                     /locus_tag="VN041_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29478.1"
                     /translation="MSTIADVAARAGVSKATASRALSGGGYVSQETRARVQ"
     gene            384..1715
                     /locus_tag="VN041_10365"
     CDS             384..1715
                     /locus_tag="VN041_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU29479.1"
                     /translation="MALSQRYRLLAPLALVGAAAIALTGCAEATPGGGNGGSEGKTTV
                     RISGGITGSEADDLNKTFAQFTEDTGIKVEYTGDKSFEGNIVTKVTGGDAPDIAIVPQ
                     PGLLKSLVETGKVQKAPEGVSKAVDENWSKDWKKYGTIDGTFYSAPMLANLKGYVWYS
                     PAKFKEWGVEVPKTWDEMMTLTKTIREKTSTAPWCAGFASGDASGWPGTDWVEDLVLR
                     QAGPDVYDKWASGDVKFTDPQIKDAFEAVGSILKNPEYVNAGFGDVKSINSTAFGDVA
                     AKVADGSCALTHQASFLSANFLDVKNAAGQTPKVAPDGDVYAFIMPGTKEGELSVEGG
                     GEFVAGFNADKSTVKVLEFMASPEFADARVKLGGVISANKNADPSLASSEFLQDAMKT
                     LQDEKTTFRFDASDLMPSTVGSGSFWKGMVDWIDGKSTDQVLSDIQAGYNN"
     gene            1807..3153
                     /locus_tag="VN041_10370"
     CDS             1807..3153
                     /locus_tag="VN041_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU29480.1"
                     /translation="MSQVIVEDEDQGTGELPQTTHGTDRRGRAITRTLVVVGFALIVA
                     LFFLLLFSTPVENASRIALGPVSLNTFFMWLGGLHPLLQIPVVLLAFGVVVAIVLVLI
                     EYAPRPGKGYFIMRLVACLVIPVLAFMLLRPYSNAVLYVVGIALVSGALLFFADYRSR
                     QGAGYLFQLILFMAPAAIMLLMGLIYPAVATFFKSFFDKTGKEFVGFENYVWVFTNPV
                     GTWSVINTLIWALLAPTISVAFGLAYAVFIDRARGEKVLKVLVFMPVAISFVGAGIIW
                     KFMYDYRQGDQLGLLNAIVTMFGGDPVNWLAVKPLLNTIMLLIVFIWTQTGFAMVILS
                     AAIKAVPAEQLEAAELDGTNAWQRFTNVTVPGIRSSLIVVLTTITIMSLKVYDIVAVM
                     TGGRDDTTVLGFEMVNQQQRFQSYGHSSALAVVLFIFVLPLIIYNARSMAKQREIR"
     gene            3153..4121
                     /locus_tag="VN041_10375"
     CDS             3153..4121
                     /locus_tag="VN041_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU29481.1"
                     /translation="MSATEAIVRDTRSKRQVARDTRRNEAIAHKKLTSKGATIAAVII
                     AFFWTIPTFGLFVTSFRPGADTQSTGWWTVFTDPKFTLDNYVQALNAGGTSTTLATAF
                     VNSLAITIPATVFPIVIASLAAYAFAWIDFKGRNLLFVFVFALQIVPLQMALVPLLSL
                     FSNGLTIGDVAVFPGFTLNDLDYSFARVWIAHAIFALPLATFMLHNFIAEIPGDIVEA
                     ARVDGAGHGQVFFRIILPLAMPAIASFGIFQFLWVWNDLLVATIFASPGALPITQALN
                     SLSGTWGNRWFLQSAGTLIAIVVPLIVFFALQRFFVRGLLAGATKG"
     gene            4227..5621
                     /locus_tag="VN041_10380"
     CDS             4227..5621
                     /locus_tag="VN041_10380"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HWU29482.1"
                     /translation="MHYAENIVDLVGDTPLVRLNRVTSGIACTVLVKLEYLNPGGSAK
                     DRIASRIIDAAEAAGDLKPGGTIVEPTSGNTGVGLALVAQQRGYRCVFVVPDKVGADK
                     IDVLRAYGAEVVVTPTSVPADSPESYYSVSDRLAREIPGAFKPNQYENPNGPRSHYET
                     TGPEIWRDTDGGITHFVAGVGTGGTISGTGRYLREVSGDRVRVIGVDPEGSVYSGGTG
                     RPYLVEGVGEDIWPGAYDPQVPNEIVAVTDAEAFAMTRRLAREEGILVGGSSGMAVVG
                     ALRVARDLPADAVMVVLLPDGGRGYLGKIFNDSWMRSYGFSEVEAEETVADVLAARDV
                     VIKLKQETHRAIPALVHTHPDETVLEAIGTMTEYDVSQLLVLSAEPPVMMGEVVGAVD
                     EKGLLDLLFRGEAQPADPVGMHVGERLPLIGIHTPVAQARSALADADALLVTVDGRPH
                     TVLTRQDLLGYLSR"
     gene            5738..6895
                     /locus_tag="VN041_10385"
     CDS             5738..6895
                     /locus_tag="VN041_10385"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HWU29483.1"
                     /translation="MTDRAFATRAIHAGQEPDPTTGAIIPPIYQASTHVQDGIGGFRE
                     GYEYNRAGNPTRSSLETQLAALEGGTSALSFSSGLAAEDALLRGILRPGDHVLLGNDV
                     YGGTYRLLTKVLAPWGIETTTVELQDADAIRAALRPETRVVWLETPSNPLLKIVDIAV
                     VAEIAHAAGAVLVVDNTFASPALQQPLALGADIVVHSTTKYLGGHSDVLGGAVVLGDD
                     RFEEAVRFQQFAVGAVSAPLDAWLTTRGIKTLAVRVRQHSENAQAIAEWAAARPEFSE
                     VYHPGLASHPGHEIAARQMSGFGGMLSLGLSGGPAAARAFAESTTLFQLAESLGGVES
                     LIGYPPEMTHASVRGTALAVPENVVRLSVGIEDVADLISDLEEGLARVAAR"
     gene            7123..8352
                     /locus_tag="VN041_10390"
     CDS             7123..8352
                     /locus_tag="VN041_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWU29484.1"
                     /translation="MLHPGDAISTGRRVLYILLLGALTALGPFTIDLYLPAFPQLEAD
                     FSTSAAMIQLTLTGTMIGFAIGQLIVGPLSDKVGRRVPLIVVTALHVLASIAAAVAPD
                     LILLGAARVVMGIGAAAGGVVAMAIVRDLFGGRRLVVMLSRLSLVSGVAPVVAPIIGS
                     GLLTVMPWRGIFVVLAGYGAVMLLAAILLLPETLPKARRQERGSSTVLQRYRSVFSDR
                     VFVGVLIIGGMTFSGLFSYLSASPFLFQQTHGLNPQAYGLLFAANSLGVVGGVQVSAR
                     LAARFGPQWVMAFSTALLLISGATIIVCDQVGAGFWGTVIPLWLFMTACGFTFPCVQV
                     LALDRHGKAAGTAASVLGASNNGVAAIISPAVGLISAGSGITATTMAIVMVGCAVVAI
                     LSLWLIVRPRTVAMLAP"
     gene            8399..9646
                     /locus_tag="VN041_10395"
     CDS             8399..9646
                     /locus_tag="VN041_10395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29485.1"
                     /translation="MTRARMVIAAGSMLAAAAVLGWAVVVLPGGAVNGLDQGWHDLMV
                     EVRRPWMLTLSYVLNYVGGGWRAGLAVPLVLIGILLLLRRWRAAVLAAAALLVSVGFA
                     QLMKALFDRARPEDMLVVSDPGSFPSGHTTNAATLAVLLYLVWPRRWTAAVGIVWTVG
                     MALSRTILSVHWLTDTVGGMLVGAAAPLLVAAVAGGWASLGWERSAAAAELKESPVSS
                     IRPYRPSDRDALYEVCVRTADVGGDATGILEDDRLWGDIFAAPYVERHPDLAWVVESA
                     DGRTIGYIVATPDTEAFETWFRDEWWPGVADRYPLSGESEPTRQDAMIGLANRRAPGQ
                     DAYSAEYPAHLHIDLLPETQGQGLGRRLIETLFDELRRRGVPGLHLGMNPANAAAGAF
                     YERLGMTLLEVTPDATYYGIRFA"
     gene            complement(9795..9882)
                     /locus_tag="VN041_10400"
     tRNA            complement(9795..9882)
                     /locus_tag="VN041_10400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9846..9848),aa:Ser,seq:gga)
     gene            complement(9940..10410)
                     /locus_tag="VN041_10405"
     CDS             complement(9940..10410)
                     /locus_tag="VN041_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU29486.1"
                     /translation="MSTVSSTRTADRTDRELLRRLIRTPGATIVALAESAGLARNTVR
                     ARLSRYDEDGTLRSFQRRIAPAALGYPLSAYVLTRVTQRKLDQVGEALSEIPEVIEVL
                     GLSGITDLMIQVVARDADDLYRVAGRILGVDGVKRTSTGLVMREMVPYRIQQLL"
     gene            10626..>11780
                     /locus_tag="VN041_10410"
     CDS             10626..>11780
                     /locus_tag="VN041_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HWU29487.1"
                     /translation="MNAFAAPVVDSIADLRTLAAIEQRVLWLSTSMIHHANRVRPNPS
                     GLKVGGHQASSASIATIMTALWFDQLQPQDRVSVKPHASPVLHGIHYLLGELDDHHMG
                     SLREFGGIQSYPSRSKDPDTVDYSTGSVGIGATAPIWGAIARRYAATISGAPAQGRQY
                     SLVGDAELDEGAVWEAVLDPHVSDLGEIVWIVDLNRQSLDRVVPNIAAGRLERMFGAA
                     GWQVITVPFGQRLEALLSHPGGAELRRRILDMPNAEYQRLLRCPAAELRHRLPGAEAS
                     ASAIRALLETVDDDELVATVRNLGGHDLATLREAFAQIDDARPTVIIAYTIKGNGLPT
                     AGHPQNHSSLLTHEQYAQLAQEVGMDPSAPWQRFEPDSPEGRACAATARRL"
BASE COUNT         1791 a         4009 c         4038 g         1942 t
ORIGIN      
        1 cctgcacgcg cgcgcgggtc tcctgcgaga cgtagccgcc gccgctcagc gcgcggctcg
       61 cggtcgcctt ggagacgccg gcacgtgcgg cgacgtcggc gatcgtgctc atcattcctc
      121 cgaatcggat gcccggtcgg gcgtccgggg tgggttcacg acagcgtatc cggatgccgc
      181 gtcgaaagga accggttcca cacgataggc tatgggagcg ctcccacgcg acgcatcacg
      241 tctccacaac ggttgtgagg atctctgtgc gtggcgttgt gcaagggcgc tcagggcaag
      301 taggttgtcc tggaatcggt tcctcaaagg ttgcggagat cgcagcctga gggccgggat
      361 ccacccgaat gaggagaaaa cacatggctc tgtcacagcg ataccgccta cttgctcccc
      421 tcgctctggt cggcgcggct gcgatcgcac tgaccggatg cgcggaggcg acgccgggcg
      481 gcgggaacgg cggctcggag ggcaagacca cggtccgcat ctccggcggc atcaccggct
      541 ccgaggccga cgacctcaac aagacgttcg cgcagttcac cgaggacacc ggtatcaagg
      601 tcgagtacac cggtgacaag agcttcgagg gcaacatcgt caccaaggtg accggtggcg
      661 acgcgcctga catcgcgatc gtcccgcagc cgggtctgct gaagtccctc gtcgagaccg
      721 gcaaggtgca gaaggccccg gaaggagtgt cgaaggcggt cgacgagaac tggtcgaagg
      781 actggaagaa gtacggcacg atcgacggca cgttctactc ggccccgatg ctcgccaacc
      841 tcaagggcta cgtctggtac tcgccggcga agttcaagga gtggggcgtc gaggttccca
      901 agacctggga cgagatgatg acgctcacca agaccatcag agagaagacg agcacggctc
      961 cgtggtgcgc gggcttcgcc tcgggcgacg cctcgggatg gccgggaacc gactgggtcg
     1021 aggacctcgt cctgcgccag gccggtcctg acgtctacga caagtgggct tccggcgacg
     1081 tgaagttcac cgacccgcag atcaaggacg ccttcgaggc ggtcggctcc atcctgaaga
     1141 atccggagta cgtcaacgcc ggcttcggcg acgtgaagag catcaactcg accgcgttcg
     1201 gcgacgtcgc cgccaaggtc gcggacggca gctgcgcgct cacgcaccag gcgtcgttcc
     1261 tgtcggcgaa cttcctcgac gtgaagaacg ccgcgggcca gacgccgaag gtcgcccccg
     1321 acggcgacgt gtacgcgttc atcatgccgg gtaccaagga gggcgagctc agcgtcgagg
     1381 gcggcggcga gttcgtcgcc ggcttcaacg cggacaagtc caccgtcaag gtgctcgagt
     1441 tcatggcatc gcccgagttc gcggacgccc gcgtcaagct cggcggcgtg atctccgcga
     1501 acaagaacgc cgacccgagc ctggcctcga gcgagttcct gcaggatgcc atgaagaccc
     1561 tccaggatga gaagacgacg ttccgcttcg atgcatccga tttgatgccc tcgaccgtcg
     1621 gctcgggctc gttctggaag ggcatggtcg actggatcga cggcaagtcg accgaccagg
     1681 tgctcagcga catccaggcc ggttacaaca actgatcggt cgcgctagtc tgagcgcaac
     1741 ctgctgacgg gtgcggggaa gggtggagac gcccggcccc gcacccgcct cacagaaggg
     1801 gaatccatgt cgcaagtgat cgtcgaagac gaggatcagg gaacaggcga gctgccgcag
     1861 accacgcacg gcacagaccg acgaggacgc gcgatcaccc gcacgctcgt cgtggtcggt
     1921 ttcgcgttga tcgtcgccct cttcttcctg ctgctgttct caacacccgt ggagaacgcc
     1981 tcacgcatcg cgctggggcc ggtctcgctg aacacgttct tcatgtggtt gggcggcctg
     2041 cacccgctgc tgcagatacc ggtcgtcctg ctcgccttcg gcgtcgtcgt ggcgatcgtg
     2101 ctggtgctca tcgagtacgc cccgcgtccg ggcaagggct acttcatcat gcggctcgtc
     2161 gcctgcctgg tgatcccggt gctcgcgttc atgctgctgc ggccgtactc gaacgccgtg
     2221 ctctacgtcg tcggaatcgc gctggtgtcc ggcgcactgc tgttcttcgc cgactaccga
     2281 tcgcgccagg gtgccggata cctgttccag ctgatcctct tcatggcacc ggccgcgatc
     2341 atgctgctga tgggcctgat ctacccggcc gtggcgacct tcttcaagtc cttcttcgac
     2401 aagacgggca aggagttcgt cggattcgag aactacgtct gggtgttcac gaacccggtc
     2461 ggcacctggt ccgtgatcaa caccctcatc tgggcgctgc tggccccgac catctcggtc
     2521 gcgttcggac tggcctacgc cgtcttcatc gaccgcgccc gcggtgagaa ggtgctgaag
     2581 gtcctcgtat tcatgccggt cgcgatctcg ttcgtgggcg ccggcatcat ctggaagttc
     2641 atgtacgact accggcaggg cgaccagctg ggtctgctga acgccatcgt gacgatgttc
     2701 ggcggcgacc ccgtgaactg gctggcggtc aagccgctgc tcaacaccat catgctgctg
     2761 atcgtcttca tctggaccca gaccgggttc gcgatggtga tcctctccgc cgcgatcaag
     2821 gccgtccccg ctgagcagct cgaggctgct gagctcgacg gcacgaacgc gtggcagcgg
     2881 ttcacgaacg tcacggtccc cggcatccgc tcctcgctga tcgtcgtcct gacgaccatc
     2941 acgatcatgt cgctgaaggt gtacgacatc gtcgcggtga tgaccggcgg tcgcgacgac
     3001 accaccgtgc tcggcttcga gatggtcaat cagcagcagc gcttccagag ctatgggcac
     3061 tcatcggcac tggccgtcgt gctgttcatc ttcgtgctgc cgctgatcat ctacaacgcc
     3121 cgatccatgg ccaagcagag ggagatccgc tgatgagcgc caccgaagcc atcgtccgcg
     3181 acacccgcag caagcgtcag gtcgcccgcg acacgcgccg caacgaggcc atcgcccaca
     3241 agaagctcac ctcgaagggc gcgacgatcg cggccgtgat catcgccttc ttctggacga
     3301 tccccacgtt cggcctgttc gtgacctcct tccgtccggg ggccgacacg cagtccacgg
     3361 gctggtggac ggtgttcacc gacccgaagt tcaccctgga caactacgtg caggcgctca
     3421 acgcgggtgg cacgtcgacc acgctggcga cggcgttcgt gaactcgctc gcgatcacga
     3481 tccccgccac cgtgttcccg atcgtgatcg cctcgctggc cgcgtacgcg ttcgcgtgga
     3541 tcgacttcaa gggccgcaac ctgctcttcg tcttcgtgtt cgcgctgcag atcgtgccgc
     3601 tgcagatggc cctggtgccg ctgctgagcc tgttctccaa cggactgacg atcggcgatg
     3661 tggcggtctt cccgggcttc acgctgaacg acctggacta cagcttcgcc cgggtgtgga
     3721 ttgcgcatgc gatcttcgcc ctgccgctgg ccacgttcat gctgcacaac ttcatcgccg
     3781 agatcccggg tgacatcgtg gaggcggctc gagtggacgg cgccggccac ggccaggtgt
     3841 tcttccggat catcctgccg ctcgcgatgc cggccatcgc ctcgttcggc atcttccagt
     3901 tcctgtgggt gtggaacgac ctgctggtcg cgacgatctt cgcctcgccc ggcgcgctgc
     3961 cgatcaccca ggcgctgaac tcgctgtcgg gcacgtgggg caaccgctgg ttcctgcagt
     4021 cggcgggcac gctgatcgcg atcgtcgtgc cgctgatcgt gttcttcgcg ctgcagcgct
     4081 tcttcgtccg cggtctgctg gccggcgcga ctaagggctg accggacgga agccggatgc
     4141 cgcaagtgcg gcctgcgcgg gaaacagagc ccgcgcaggc tgaaacacaa gcggcatccg
     4201 gcttccgtgc atctacggtg gaccgcatgc actatgccga gaacatcgtc gacctggtcg
     4261 gcgacacgcc cctggtcagg ctcaaccggg tgacctccgg catcgcctgc accgtcctgg
     4321 tcaagctcga gtacctgaac cccggcggct ccgccaagga ccgcatcgcc agtcgcatca
     4381 tcgacgcggc cgaggctgcc ggcgacctga agccgggcgg cacgatcgtc gagcccacca
     4441 gcggcaacac cggggtcggg ctggcactgg tggcgcagca gcgcggttac cgctgcgtgt
     4501 tcgtggtgcc cgacaaggtg ggtgcggaca agatcgacgt gctgcgcgcc tacggggccg
     4561 aggtcgtcgt gacgccgaca tcggtccccg ccgacagccc cgagtcgtac tacagcgtca
     4621 gcgatcggct cgcccgcgag atccccggtg cgttcaagcc caaccagtac gagaacccca
     4681 acgggccgcg cagccactac gagaccacag ggcccgagat ctggcgcgac accgacggtg
     4741 ggatcaccca cttcgtcgcc ggcgtcggca cgggcggcac gatcagcggc accggccggt
     4801 acctgcgcga ggtatcgggc gaccgcgtgc gcgtgatcgg cgtcgacccc gagggcagcg
     4861 tgtactcggg cgggaccggc cggccgtatc tcgtcgaggg tgtgggcgag gacatctggc
     4921 ctggggccta cgacccgcag gtgccgaacg agatcgtcgc cgtcacggat gccgaggcgt
     4981 tcgccatgac gcgccggctc gcccgcgagg agggcatcct cgtcggcgga tccagcggga
     5041 tggcggtggt cggcgccctg cgggtcgcgc gcgacctgcc cgcggacgcc gtgatggtgg
     5101 tgctgctgcc ggacggcggg cgggggtacc tgggcaagat cttcaacgat tcctggatgc
     5161 gctcctacgg cttcagcgag gtggaggcgg aggagaccgt cgcagacgtg ctggccgcgc
     5221 gcgacgtcgt gatcaagctc aagcaggaga ctcaccgagc gatccccgcg ctggtgcaca
     5281 cgcatcccga cgagacggtg ctcgaggcaa tcggaaccat gaccgagtac gacgtctcgc
     5341 agctgctggt gctcagcgcc gagccgcccg tgatgatggg cgaggtcgtc ggcgccgtgg
     5401 atgagaaggg gctgctcgac ctgctgttcc gaggcgaggc gcagcccgcc gacccggtgg
     5461 gcatgcacgt gggggagcgg ttgccgctca tcggcatcca caccccggtc gcgcaggcgc
     5521 gcagcgcgct cgccgacgcg gacgccctgc tcgtcacggt cgacgggcgc ccgcacacgg
     5581 tgctcacccg tcaggatctg ctcggatacc tctcccgctg aggacatctg ggtccctgaa
     5641 cctgtcgaag ggtcgtctcg cgacgtaaca ggacgacgga tgcccgggtc gccgggcgta
     5701 ccgtgagcac accgccacac acgacctgag gagagtcatg accgaccgcg ccttcgccac
     5761 ccgagccatc cacgcgggtc aggagcccga tccgacgacc ggcgcgatca tcccgccgat
     5821 ctaccaggcg tccacgcacg tgcaggacgg catcggcggc ttccgggagg gctacgagta
     5881 caaccgcgcg ggaaacccca cgcgctcgtc gctcgagacg cagctcgcgg ctctggaggg
     5941 cggcacctcg gcgctgtcgt tctcgtcggg gctcgccgcc gaggatgcgc tgctgcgcgg
     6001 catcctgcgc cccggcgacc atgtgctgct cggcaacgac gtgtacggcg gcacctaccg
     6061 cctgctcacg aaggtgctgg cgccctgggg catcgagacc accacggtgg agctgcagga
     6121 tgcggatgcc atccgcgcgg cgctgcggcc cgagacgagg gtcgtgtggc tggagacccc
     6181 cagcaaccca ctgctgaaga tcgtcgacat cgcggtggtc gcggagatcg cacacgccgc
     6241 cggtgcggtg ctggtggtgg acaacacgtt cgcctcgccg gcgctgcagc agccgctcgc
     6301 gctcggggcc gacatcgtgg tgcactcgac caccaagtac ctcggcgggc actccgacgt
     6361 gctcggcggg gcggtggtgc tgggtgacga caggttcgag gaggctgtgc gattccagca
     6421 gttcgccgtg ggcgcggtgt ccgcgcccct ggatgcctgg ctcacgacgc gcggcatcaa
     6481 gaccctcgcc gtgcgagtgc gtcagcacag cgagaacgcc caggcgatcg ccgagtgggc
     6541 ggcggcgcgg ccggagttct ccgaggtcta tcaccccgga ctcgcctcgc accccggcca
     6601 cgagatcgcc gcacgccaga tgagcggttt cggcgggatg ctgtcccttg ggctttccgg
     6661 agggcccgcc gcagcgcgcg cattcgccga atcgaccacg ctgttccagc tcgcggagtc
     6721 gctcggcggc gtggagtcgc tgatcggcta cccgccggag atgacgcacg cctcggtgcg
     6781 cggcaccgcg ctcgccgtcc ccgagaacgt ggtgcgtctg tccgtcggga tcgaggacgt
     6841 cgccgacctc atctccgacc tggaggaggg tctggcccgc gtcgcggcac gctgaggccg
     6901 cccgcgcgga gtccgcggcg ggctgcgcgg gcgagtaaca tgggggactt cccctgcgtc
     6961 gaatcgattc gagcgcagca tccgctcctc ccttgaaagt tccccgtgag cgacgaccgc
     7021 actcccaccg gcagcatccg ggccatcacc ggaaccatcc gcacctccac gccgaccggc
     7081 gcgatccgca ccctcggggc gaacccggcg accgcgccga tcatgctgca cccgggagac
     7141 gcgatctcca ccggacgccg ggtgctctac atccttctgc tcggcgcgct cacggcgctc
     7201 gggccgttca cgatcgacct gtacctgccg gcgttcccgc aactggaggc ggacttctcc
     7261 acctcggccg cgatgatcca gctgaccctc accggcacca tgatcggctt cgcgatcggg
     7321 cagctgatcg tcgggccgct cagcgacaag gtcgggcgcc gggtgccgct gatcgtggtg
     7381 accgcactgc acgtgctcgc cagcatcgcc gccgccgtcg cgccggatct gatcctgctg
     7441 ggggcggcgc gcgtggtgat gggcatcggc gctgccgccg gcggcgtggt ggccatggcg
     7501 atcgtgcgcg acctgttcgg cggccggcgc ctggtcgtga tgctctcccg gctgtcgctg
     7561 gtgtcgggcg tcgctcccgt ggtcgcgccg atcatcggct cgggactgct gacggtcatg
     7621 ccgtggcggg gcatcttcgt ggtcctggcc ggctacggcg ccgtgatgct gctcgcggcg
     7681 atcctgctgc tgcccgagac gctgccgaag gcgcgccggc aggagcgcgg gtccagcacc
     7741 gtgctgcagc gctaccgcag cgtgttctcc gatcgggtgt tcgtgggcgt gctgatcatc
     7801 ggcggcatga cgttctccgg cctgttctcg tacctgtcgg cctcgccgtt cctgttccag
     7861 cagacgcacg gactgaaccc ccaggcctat gggctgctgt tcgcggcgaa ctcgctcggc
     7921 gtggtcggcg gcgtgcaggt gtccgcgcgg ctggcggcgc ggttcgggcc gcagtgggtg
     7981 atggcgttct cgacggctct gctgctgatc tcgggcgcga cgatcatcgt gtgcgatcag
     8041 gtgggtgccg ggttctgggg caccgtgatc ccgctgtggc tgttcatgac ggcctgcgga
     8101 ttcacgttcc cctgcgtgca ggtgctcgcg ctggaccggc acggcaaggc cgccggcaca
     8161 gcggcgtccg tgctcggtgc gagcaacaac ggcgtcgccg cgatcatctc gcccgccgtc
     8221 gggctgatct cggcgggctc cggcatcacc gcgaccacca tggcgatcgt catggtggga
     8281 tgcgcggtgg tggccatcct gtcgctgtgg ctgatcgtgc gcccgcgcac ggtcgcgatg
     8341 ctcgcgccct gacgcgcgtg atgccgggtc gatcgggccg ggcaggcaga atgggacgat
     8401 gaccagggcc aggatggtga tcgcggccgg cagcatgctg gcggccgccg ccgtgctcgg
     8461 ctgggccgtc gtggtgctgc ccggtggggc ggtgaacggc ctcgatcagg gatggcacga
     8521 tctgatggtc gaggtcaggc ggccgtggat gctgacgctg tcgtacgtgc tgaactacgt
     8581 cggcggggga tggcgggcgg gcctcgccgt gcccctggtg ctgatcggga tcctcctgct
     8641 gctgcgacgc tggcgggccg cggtgctcgc ggctgccgcc ctgctggtga gcgtgggatt
     8701 cgctcagctg atgaaggcgc tgttcgaccg tgcgcgaccg gaggacatgc tcgtcgtctc
     8761 ggaccccggt tcattcccct ccgggcatac gacgaacgcc gccacgctcg cagtgctgct
     8821 gtacctcgtc tggcctcggc gctggactgc agcggtcgga atcgtctgga ccgtcggcat
     8881 ggcgctgtcg cgcacgatcc tcagcgtgca ctggctcacc gacaccgtcg gcgggatgct
     8941 ggtcggcgcc gcggccccgc tgctggtcgc ggcggtggca ggcggctggg cctcactagg
     9001 ctgggaacgt tccgcggcag cagcggagct gaaggagtca cccgtgtcga gcatccgccc
     9061 ttaccgtccc tccgaccgtg acgcactgta cgaggtgtgc gtgcggacgg cggatgtcgg
     9121 cggtgacgcg accgggatcc tggaggacga tcgtctctgg ggtgacatct tcgcggcgcc
     9181 ctatgtggag cggcatccgg atctggcctg ggtcgtggag tccgccgacg gacgaaccat
     9241 cggctacatc gtcgccacgc ccgacaccga ggcgttcgag acgtggttcc gcgacgagtg
     9301 gtggccgggc gtcgcggacc gctacccgct gtcgggtgag agcgagccga cgcgacagga
     9361 cgccatgatc gggctcgcga accgccgggc gccggggcag gacgcctact ccgcggagta
     9421 cccggcgcac ctgcacatcg atcttctgcc cgagacgcag ggtcaggggc tggggcgccg
     9481 gctgatcgag accctcttcg acgaactgcg ccgccgcggt gtgccgggcc tgcacctggg
     9541 gatgaacccc gccaacgcgg ccgccggtgc gttctacgag cgtctgggga tgacgctgct
     9601 cgaggtcacg cccgacgcca cgtactacgg catccgcttc gcctgagcgc aaagcggtgc
     9661 gcgagtgcgc cggatgccgc cgagtgaacc accaggcgac gtcgacaccc ggtgcagtcg
     9721 acccgaagcg gtgcattcgg cgctggagcg gaaacgcctg agggccccca cttcgtgagg
     9781 gccctcaggc gttctggcgg tgacggcggg attcgaaccc gcggttgctt gcacaacaca
     9841 cgctttccaa gcgtgctcct tcggccgctc ggacacgtca ccaggaaaca acctgtccat
     9901 cctatccgat gcggaagccg ctgatgaaca cagcgagcct cagagcagct gctggatgcg
     9961 gtagggcacc atctcgcgca tcaccaggcc cgtgctggtg cgcttgacgc cgtcgacgcc
    10021 gagaatgcgg ccggcgaccc ggtacaggtc gtcggcgtct cgcgcgacga cctggatcat
    10081 gaggtcggtg atccccgaaa gaccgagcac ctcgatcacc tcggggatct cggacagcgc
    10141 ctcaccgacc tgatcgagct tccgctgcgt gacccgggtg agcacgtacg cgctcagcgg
    10201 gtagcccagc gccgccgggg cgatgcggcg ctggaaggag cgcagcgttc cgtcctcgtc
    10261 gtatcgggag aggcgtgcgc ggacggtgtt gcgtgcgagt cctgccgatt cggcgagggc
    10321 gacgatcgtc gccccggggg tgcggatgag ccggcgcagc agctcccggt cggtgcgatc
    10381 agcggttctg gtgctggaca cagtgctcat catcctggct ctcgatcgcg ggattctgcc
    10441 cgttctgctc aatatcgacg acaactattg tccatcacgg cgcctgttgc tccaatgggg
    10501 gaaacatctg cttcatctcc gggcatcggc gcgcatcatg ctccttcagc ctgcggcccc
    10561 agaagggccg gagcatccgc ggatgacggc gaggacgccg catcccattc cgaggaaccc
    10621 gtcgcatgaa cgccttcgct gcacccgtcg tcgactccat cgcagatctc cgcacgctcg
    10681 ccgcgatcga gcagcgcgtg ctgtggctgt cgacgtcgat gatccaccac gccaaccgcg
    10741 tccgtcccaa cccgtccggg ctcaaggtgg gaggacacca ggcgtccagc gcgtccatcg
    10801 cgacgatcat gacggcgctg tggttcgacc agctccagcc ccaggatcgc gtgtcggtga
    10861 agccgcacgc ctctccggtg ctgcacggca tccactatct gctcggcgag ctcgacgacc
    10921 accacatggg ctccctgcgc gagttcggcg gcatccagag ctatccgagc cggtcgaagg
    10981 accccgacac cgtcgactac tccaccggct cggtggggat cggcgccacc gcgcccatct
    11041 ggggcgcgat cgcccgccgc tatgcggcca cgatctcggg ggcgccggcg caggggaggc
    11101 agtactcgct tgtcggggat gccgaactcg atgagggtgc ggtctgggag gcggtgctcg
    11161 accctcatgt gtccgacctc ggcgagatcg tctggatcgt cgacctgaac cggcaatcgc
    11221 tcgaccgggt ggtgccgaac atcgccgcag gcaggctgga gcggatgttc ggcgccgcgg
    11281 gatggcaggt catcaccgtg ccgttcgggc agcgactcga agcgctcttg tcgcatccgg
    11341 gaggcgccga gctccgccgg cggatcctcg acatgcccaa cgcggagtac cagcggctgc
    11401 tgcgctgccc ggcggcggag ctgcgccacc gcctgcccgg ggccgaggcg tcggcttccg
    11461 ccatccgtgc tctgctcgag accgtggacg acgacgaact cgtcgccacg gtccgcaatc
    11521 tcggcgggca tgacctggcg acactgcgcg aggcgttcgc gcagatcgac gacgcgcgcc
    11581 cgacggtgat catcgcgtac acgatcaagg ggaacgggct gcccacggcc gggcatccgc
    11641 agaaccactc ctcgctgctc acgcacgagc agtacgcgca gctcgcgcag gaggtcggga
    11701 tggacccgtc cgccccgtgg cagcggttcg agcccgactc gccggagggg cgggcgtgcg
    11761 ctgccaccgc gcggcgactc
//
LOCUS       DATMSX010000334         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_62427,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000334 DATMSX010000000
VERSION     DATMSX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62427"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VN041_10415"
     CDS             complement(<1..547)
                     /locus_tag="VN041_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU29488.1"
                     /translation="MSTSFDERERPTARSTPGARATRTGLIALAVPHLEEPYFAELSS
                     RLVRGAEARGLAVLIVQTEGEHSREIDVANGHGLPDIDGLIHIPRSLTVADLTRRTSP
                     GPLVLLGEHIQVSPFTHITIDNCGAAKTATAHLIEVGCRRIAFIGRRDARPSDAADRR
                     HAGYLEALEAAGIPADPALTAQ"
     gene            complement(616..>1536)
                     /locus_tag="VN041_10420"
     CDS             complement(616..>1536)
                     /locus_tag="VN041_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HWU29489.1"
                     /translation="ASGWQRTIAAGADAAGAGDAAPHVLAEDLGIVVPVHPLLETTDL
                     LLSASESTTLTVEVWSTGKRQNVVPAHLEHSATIDVPAGDAAWVSAPTPFAPETPQNA
                     IVVLRANPSLSVHLTSPLPAGVLTLVHRVDNDDENVEISDDGLLVKWPTKPLRGRAPI
                     FRALPESEALAPERAVSGYNRPFGGPNMWASGPLSDGPAWLRLDWAAPVTAREVRLVF
                     DDDVDVSLITLHHHRTPDEIMPQLVRDYRVEVLVDGTWVTAVAEQGNRLRHRVHPLPA
                     DLGAFTAARLVIEATNGAAEARVIAFRVQS"
BASE COUNT          203 a          534 c          576 g          223 t
ORIGIN      
        1 cctgggcggt cagcgccgga tcggcgggga taccggcggc ctccagcgcc tcgagatagc
       61 cggcgtgccg gcgatccgcg gcatccgagg ggcgcgcgtc gcgacgcccg atgaaggcga
      121 tgcgacggca cccgacctcg atcaggtgcg cggtcgcggt cttcgccgcg ccgcagttgt
      181 cgatcgtgat gtgcgtgaac gggctcacct ggatgtgctc gcccagcagt accagggggc
      241 cgggggaggt gcgacgcgtc agatcggcga cggtcagcga gcgcgggatg tggatcaggc
      301 cgtcgatgtc gggcaggccg tggccgttgg cgacgtcgat ctcgcggctg tgctcgccct
      361 cggtctgcac gatgagcacg gcgagtccgc gcgcctcggc cccgcgcacc aggcgcgagc
      421 tgagctcggc gaagtacggc tcctccaggt ggggcacggc cagcgcgatc agcccggtgc
      481 gggtcgccct ggcgcccgga gtcgagcggg cggtcgggcg ctcccgctcg tcgaaggacg
      541 tgctcaccct cggtctcctc ttggcgcgac ggtcacccgc gggaacgcgg gtggacttca
      601 cgttagcgtc atccgtcacg actgcacccg gaatgcgatc acgcgcgcct cggccgcgcc
      661 gttggtcgcc tcgatcacga gccgtgccgc ggtgaaggca ccgaggtccg caggcagagg
      721 atgcacccga tggcggagcc ggttgccctg ctccgcgacg gccgtgaccc aggtcccgtc
      781 gaccagcacc tcgacgcggt agtcgcgcac cagctgcggc atgatctcgt cgggtgtgcg
      841 gtggtggtgc agcgtgatca gtgagacgtc gacgtcgtcg tcgaacacca gacgcacttc
      901 gcgggccgtc acgggggcag cccagtccag ccgaagccag gcgggcccgt ccgacagcgg
      961 accggaggcc cacatgttcg gaccgccgaa gggacggttg taccccgaca ccgcacgctc
     1021 gggtgcgagc gcctcgctct cgggcagcgc gcggaagatc ggcgcgcggc cgcgcagggg
     1081 cttggtcggc cacttcacga gcaggccgtc gtcgctgatc tcgacgttct cgtcgtcgtt
     1141 gtccacccgg tgcacgagcg tgagcacgcc cgcggggagc ggcgaggtca ggtgcacgct
     1201 caggctcgga ttcgcccgca gcacgacgat cgcgttctgc ggcgtctcgg gcgcgaacgg
     1261 cgtcggcgcg ctcacccagg cggcgtcgcc ggcgggcacg tcgatcgtgg cgctgtgctc
     1321 gagatgcgcg ggcacgacgt tctgccgctt tccggtggac cagacctcca cggtcagcgt
     1381 ggtggactcg ctcgcgctga gcagcaggtc ggtggtctcc aggagcggat gcacgggcac
     1441 gacgatcccg aggtcctcgg cgagcacgtg cggtgcggcg tctcctgccc ccgcggcatc
     1501 cgctccggcc gcgatcgtgc gctgccagcc cgacgc
//
LOCUS       DATMSX010000335         6360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_33609,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000335 DATMSX010000000
VERSION     DATMSX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33609"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VN041_10425"
     CDS             complement(<1..378)
                     /locus_tag="VN041_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU29490.1"
                     /translation="MIDPARLRSDAAASLPQLIALRRSLHRTPEPGFSLVRTRDAVLR
                     ALQGLDLEIVAGPDDFSSITAVLRGARPGPSVLLRADMDALRVGEETGLPYSSEVEGV
                     SHACGHDLHTALPCAWRSSRSAIR"
     gene            484..1164
                     /locus_tag="VN041_10430"
     CDS             484..1164
                     /locus_tag="VN041_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWU29491.1"
                     /translation="MARVTNAAVAVRVARLLEEEITSSGGKPGDFIGRRQELAARFSV
                     APATLGEAIQLLRSRGVVDAKPGPGGGIFVADRSPFMRLSDQLLSLREGQATVADCLR
                     VLDALDAPVLHDAVDFATEADVEDVRLLAERLEASWGTPRSAAANWALHARIAQITPN
                     ELLRSLYTNLVDYIIEEELEGATAPTTAERLRTHLDFAAAIVSHDHGLATSALERHRG
                     SSMAAVLP"
     gene            1273..2319
                     /locus_tag="VN041_10435"
     CDS             1273..2319
                     /locus_tag="VN041_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU29492.1"
                     /translation="MIEFRSVIDEMLATPNRPAGATPPPIRLSQNEMPWRPADVIVDA
                     MVAAVENAHRYPDYHRAAARRAIADSIGVDADRIAIDNGSGSLLHGAARLVCEPGADG
                     VYCWPSFEAYVAALGLAGAHRTPVGLAADGAMDLPAMLAAIGDDTRIVYLCNPNNPTG
                     GFVAAAEIRAFLAQVPANVLVVLDEAYREFVTAEPVEATTALLDEFENLIILRTLSKS
                     HGLAGVRAGYAVSSPRIAEALRRTSVGFALNSLAEAAVIAAFGADAQAIADERIAIIV
                     SERERMQAALTDAGVSFLPSQSNFIFIHGDAGELMRRFADQGVLARPFPQSGGVRVTV
                     GLPSENDAALAALL"
     gene            2479..4290
                     /locus_tag="VN041_10440"
     CDS             2479..4290
                     /locus_tag="VN041_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWU29493.1"
                     /translation="MTATIDFQRDRRASIRRSATSTALWALFVFVLALVFIYPVSMIV
                     IGAFRDGLPSDNAPFTIQGLIVAFQDPGTWQTMWNSVVLVLVCGTISVAGGGLFAWMA
                     ANTNVPGRRMLTGLMALNLFVPPLFHTLGWIMLGNPQNGLLNQFGRALGMTGPLLNIQ
                     SWGGLILLLSLGYMPFAYLLMLGAFKNRDQSLDEAAAISGAGTARTFFTITIPSVGPA
                     ITGAAILVAVLVFQSFDSPQLIGRQANIFVFSTQIYHYVRDQAPAEYTKAFALSLVLI
                     ILVLVLFLAQRWLLRGRSFTTLTGKTSRREPQELGPVRWVLSALIVVFMLLNLVLPLF
                     AMILGSLQPIFGVMSTGLTFDNYVQMLTDPQFRDSLVLTAWLAIIGGFGSIAVALLTT
                     YVTARRTGFIRSYTSFAVWIPWALPGIVLALAYMFAVLYLPAFRSLYGTAFLMTLVLI
                     VATIPVSSRIAEGALAQLSPELEEAARTAGAATPRVLLTIVFRLVIPSFLSGWFLSAL
                     FISGNLAVPMLLAPPGLQPVSLTAFEQFLLGNMSQGAALFLIILVAAMAVLGLAALSM
                     KIGTLLRDKPGYAPSPVISGSAQPSVLYPNTTHREEE"
     gene            4290..5417
                     /locus_tag="VN041_10445"
     CDS             4290..5417
                     /locus_tag="VN041_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU29494.1"
                     /translation="MKFSRTTMKITTAVLASGMLLTLAACSSGGDPAPKSDKPVEVPG
                     ASADGNAYMNELYDKAVKDGKTDVVLYGPSVTAQKALFKVFTDRFPGIKIVPQNQPDA
                     QSITKIETEAQSGNKIADIYDGGESPQVAAKPDLCTKADVRTGPDGFEIPTNMDGRLT
                     YYAMRYFVFVYNTDMVKESEAPKNWHDLLDPKWKGKITIGDPTVPGGVRYILTSLMVD
                     EGGSAEKWGKPFMEKLAKQDLQFAQSEPNVPSDVASGRFPIGIAVYQGFYEDTKAKGA
                     PIAAVFPLDDGGNFMSRTGMCMVKESPHADAAQLYINWLYSPEGQQALAEKNNSYGWT
                     PAAPGPKGAPALEKLGRLPFSNPDPEFNKPYFEFLDSQFKK"
     gene            5458..>6360
                     /locus_tag="VN041_10450"
     CDS             5458..>6360
                     /locus_tag="VN041_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29495.1"
                     /translation="MTEVLVSGLTKRYGSKSALHGLDLTIGSGEFVTLLGPSGCGKTT
                     TLRCLAGLERPTTGRVVMGERTVVDIERRTFVPADKRQVGMVFQSYALWPHMSVSENV
                     AYPLKVARVPTRERIARVGEMLDAVGLAGYEKRPVTALSGGQQQRVALARALAARPGI
                     IFYDEPLSNLDSKLRYQMGQQVRALHNQFQTTSVYVTHDQEEAITLSDRIIVMSEGEI
                     VQDGAPSELYDHPRSTYVADFMGFQNILPGTVVAVSDGRADIRVDDTSLVVTGVATTD
                     VVVGERADVAFRAAHVRVDRHPSAS"
BASE COUNT         1008 a         2246 c         2083 g         1023 t
ORIGIN      
        1 ccgaatcgct gagcgtgaac ttctccacgc gcaaggtaag gccgtgtgca gatcgtgacc
       61 gcaggcgtgc gagacgccct cgacctcgga ggagtacggc aggccggtct cctccccgac
      121 gcggagcgca tccatgtccg cgcgcagcag cacggacgga ccggggcggg ccccgcggag
      181 gacggcggtg atcgaggaga agtcgtcagg cccggcgacg atctccaggt ccaggccctg
      241 cagggcgcgg agcacggcat cgcgggtccg gacgagggag aagccgggct cgggtgtgcg
      301 gtgcagcgac cggcgcaggg cgatcagctg ggggagcgag gccgcggcat cgctgcggag
      361 acgtgcggga tcgatcatgg gcctcctcgc cggcggtcga cgcgcgacag cggcgtcgaa
      421 taagatcggt tcaatatagc gaatcacatt cgtgatgcgc ggccgggtcc ggaggggaat
      481 gcaatggcgc gggtcacgaa cgccgctgtg gccgtgcgag tcgcacggct gctggaggag
      541 gagatcacct cgagcggcgg caagccgggg gacttcatcg gtcgccgcca ggagctcgcc
      601 gcgcggttct ccgtcgcgcc ggccacgctc ggcgaggcga tccagctgct gcgctcgcgc
      661 ggcgtggtcg atgccaagcc ggggccgggt ggcgggatct tcgtcgccga caggtcgccc
      721 ttcatgcggc tcagcgatca gctgctcagc ctgcgcgagg gtcaggccac cgtcgccgac
      781 tgcctgcggg tcctcgacgc cctcgatgcg cccgtgctgc acgacgccgt ggacttcgcc
      841 accgaggcgg atgtcgagga tgtccggctg ctcgcggagc ggctggaggc gagctggggc
      901 acaccgcggt cggcggccgc gaactgggcc ctgcacgcgc ggatcgctca gatcacgccg
      961 aacgagctgc tgcgcagcct ctacaccaac ctcgtcgact acatcatcga ggaggagctc
     1021 gagggggcga cggcgccgac gacggcggag cgcctgcgca cccacctcga cttcgccgcg
     1081 gcgatcgtct cgcacgacca cggactcgcg acctcggcgc tcgagcgcca tcgcgggtcc
     1141 tccatggccg ccgtgctgcc ctgaggcatc cggcgaaatg atggctccgc cggtttgaca
     1201 gcatccgcat cgatgaataa tctacatatt cattattaac aattattcct ggctgcttcg
     1261 gaggagtcgc agatgatcga gttccgcagc gtgatcgatg agatgctcgc cacgcccaac
     1321 cggcccgcag gtgcgacgcc tcccccgatc cggctctctc agaacgagat gccgtggcgc
     1381 ccggcggacg tgatcgtcga tgccatggtg gccgccgtgg agaacgcgca ccgctatccc
     1441 gactaccacc gtgccgcggc ccggcgcgcg atcgccgact cgatcggcgt ggacgccgac
     1501 cgcatcgcca tcgacaacgg atcgggatcg ctgctgcacg gcgccgcgcg gctggtgtgc
     1561 gaacccggtg ccgacggcgt ctactgctgg ccgtcgttcg aggcgtacgt ggcggcgctg
     1621 gggctcgccg gtgcgcaccg cacccccgtc ggcctggccg ccgatggcgc catggatctg
     1681 cccgccatgc tcgcggcgat cggcgatgac acccgcatcg tttacctgtg caatccgaac
     1741 aaccccaccg gcggcttcgt cgcggcggcg gagatccgcg ccttcctcgc gcaggtcccc
     1801 gcgaacgtgc tcgtcgtgct cgacgaggcg taccgcgagt tcgtcaccgc cgagccggtc
     1861 gaggccacca cggcgctgct cgacgagttc gagaacctga tcatcctgcg cacgctgtcg
     1921 aagtcgcacg ggctcgcggg agtgcgcgcc ggatacgccg tctcgtcccc gcgcatcgcg
     1981 gaggccctgc ggcgcacctc ggtcggattc gccctgaaca gcctggccga ggccgcggtc
     2041 atcgccgcct tcggcgcgga cgcgcaggcg atcgccgacg agcggatcgc gatcatcgtc
     2101 tccgagcgcg agcggatgca ggcggcgctg accgacgcgg gggtatcgtt cctgccgagt
     2161 cagtcgaact tcatcttcat ccacggcgac gcgggcgagc tgatgcgccg cttcgccgat
     2221 cagggcgtgc tggcacgccc gttcccgcag tccgggggag tccgcgtgac ggtggggctt
     2281 ccgagcgaga acgacgcggc gctggctgca ctgctgtaac aaggcgctcc gacacagtgt
     2341 cggaaccgcc gcagaggact tcggagcggg aacggatgcc cgctccgagg gtccgttcat
     2401 acgctgtcat gacagatgga cgcagtggtc ccgcccgagt ggaccgcggt gatccgagga
     2461 gcccgaaggg atgtgggagt gactgccacg atcgatttcc aacgcgatcg gcgggcgtcg
     2521 atccggcgct ccgcgacgag caccgccctg tgggccctgt tcgtcttcgt gctcgcgctg
     2581 gtgttcatct acccggtctc gatgatcgtc atcggcgcgt tccgcgacgg tctcccgtcc
     2641 gacaatgccc cgttcacgat ccagggtctg atcgtcgcgt tccaggaccc cggcacgtgg
     2701 cagaccatgt ggaactcggt cgtcctcgtg ctggtctgcg gcacgatctc ggtcgccggc
     2761 ggcggactgt tcgcctggat ggcggcgaac acgaacgttc ccggacggcg gatgctgacc
     2821 ggcctgatgg cgctgaacct gttcgtgccg cccctgttcc acactctcgg ctggatcatg
     2881 ctcggcaacc cgcagaacgg cctgctgaac cagttcggac gtgcgctggg catgacggga
     2941 ccgctgctga acatccagtc ctggggcgga ctgatcctgc tcctgtcgct cggctacatg
     3001 ccgttcgcct acctgctgat gctgggcgcc ttcaagaaca gggaccagtc cctcgacgag
     3061 gccgcggcca tcagcggcgc gggcacggca cgcacgttct tcacgatcac catcccctcg
     3121 gtgggccccg ccatcacggg tgcggcgatc ctcgtcgccg tgctggtgtt ccagtccttc
     3181 gacagcccgc agctgatcgg gcggcaggcg aacatcttcg tgttctcgac gcagatctac
     3241 cactacgtgc gggatcaggc gccggcggag tacaccaagg cgttcgccct ctcgctggtg
     3301 ctgatcatcc tggtgctcgt gctgttcctc gcccagcgct ggctgctgcg cgggcgcagc
     3361 ttcacgacgc tcaccgggaa gacctcgcgg cgtgagccgc aggagctcgg cccggtgcgc
     3421 tgggtgctct ccgccctgat cgtcgtcttc atgctgctca acctcgtgct gccgctgttc
     3481 gcgatgatcc tcggcagcct gcagccgatc ttcggcgtca tgagcaccgg cctcacgttc
     3541 gacaactacg tgcagatgct caccgacccg cagttccgcg acagcctggt gctgacggcg
     3601 tggctggcca tcatcggcgg cttcggctcg atcgcggtcg cgctcctgac cacctacgtc
     3661 acagctcgcc gcaccggctt catccgctcc tacacctctt tcgcggtgtg gatcccgtgg
     3721 gcgctgcccg gcatcgtcct ggcgctggcg tacatgttcg ccgtcctcta cctgccggcg
     3781 ttccgcagcc tctacggcac ggccttcctg atgacgctcg tgctcatcgt cgccacgatc
     3841 ccggtctcca gccggatcgc cgaaggcgcg ctcgcccagc tctccccgga gctcgaggag
     3901 gcggcgcgca ccgcgggcgc cgcgaccccg cgggtgctgc tgaccatcgt cttccgcctg
     3961 gtcatcccca gcttcctgtc cggatggttc ctgtcggcgc tgttcatctc cggcaacctc
     4021 gcggtgccga tgctgctggc gccccccggg ctgcagccgg tgtcgctgac ggccttcgag
     4081 cagttcctgc tcggcaacat gtcgcagggt gcggcgctgt tcctgatcat cctggtcgcc
     4141 gccatggccg tgctcggcct cgccgccctg tccatgaaga tcggcaccct gctgcgagac
     4201 aagcccggat acgctccgtc gcccgtgatc tccggatcgg ctcaaccctc agttctgtac
     4261 ccgaacacca cccaccgaga ggaagaatga tgaagttctc ccgcaccacc atgaagatca
     4321 ccacagcagt gctcgccagc ggaatgctgc tgaccctcgc cgcctgctcg tccggcggcg
     4381 accccgcccc gaagtccgac aagccggtcg aggtccccgg cgccagtgcc gacggcaacg
     4441 cctacatgaa cgagctctac gacaaggccg tcaaggacgg caagaccgac gtcgtcctct
     4501 acggccccag cgtcaccgcg cagaaggccc tgttcaaggt gttcaccgac cgcttccccg
     4561 ggatcaagat cgttccgcag aaccagccgg acgcgcagag catcacgaag atcgagaccg
     4621 aggcgcagag cggcaacaag atcgccgaca tctacgacgg cggcgagtcg ccgcaggtgg
     4681 cggccaagcc cgacctgtgc accaaggcgg atgtgcgcac cggtcccgac gggttcgaga
     4741 tcccgaccaa catggacggc cggctcacct attacgccat gcgctacttc gtcttcgtct
     4801 acaacaccga catggtgaag gagagcgagg cacccaagaa ctggcacgac ctgctcgacc
     4861 cgaagtggaa gggcaagatc accatcggcg acccgacggt ccccggtggc gtgcgctaca
     4921 tcctcacctc gctgatggtg gacgaaggcg gcagcgccga gaagtggggc aagccgttca
     4981 tggagaagct cgccaagcag gacctccagt tcgcccagtc cgagcccaac gtcccctcgg
     5041 acgtcgccag cggccgcttc ccgatcggca tcgccgtcta ccagggcttc tacgaggaca
     5101 ccaaggcgaa gggtgctccc atcgcggccg tcttcccgct cgacgacgga ggcaacttca
     5161 tgtcccgcac cggcatgtgc atggtgaagg agagcccgca cgccgacgcc gcccagctgt
     5221 acatcaactg gctgtactcg cccgaggggc agcaggcgct cgccgagaag aacaactcct
     5281 acggctggac tccggcggct cccggtccga agggcgctcc cgcgctcgag aagctcggtc
     5341 gcctgccctt ctccaacccc gaccccgagt tcaacaagcc ctacttcgag ttcctggact
     5401 cccagttcaa gaagtagtcg cttaccttct tcaatcctgt agagagcgtg gatcagcatg
     5461 actgaggtcc tggtatccgg actgaccaag cggtacggca gcaagtcggc actgcacgga
     5521 ctcgatctga cgatcggatc cggcgagttc gtcaccctcc tcggcccttc gggctgcggg
     5581 aagacgacga cgctgcgctg cctggccggc ctggagcgtc ccaccacggg ccgagtcgtg
     5641 atgggggagc ggacggtcgt cgacatcgag cggcgcacct tcgtgccggc cgacaagcga
     5701 caggtcggca tggtcttcca gagctacgcc ctgtggccgc acatgagcgt gtcggagaac
     5761 gtcgcgtacc cgctcaaggt ggcgcgcgtc cccaccaggg agcgcatcgc gcgcgtcggc
     5821 gagatgctcg acgcggtcgg cctcgcagga tatgagaagc gtcccgtcac cgccctgtcg
     5881 ggcggccagc agcagcgcgt cgcactggcg cgcgccctgg ccgcccgacc gggcatcatc
     5941 ttctacgacg agccgctgtc gaacctcgac agcaaactgc gctatcagat ggggcagcag
     6001 gtcagggcac tgcacaacca gttccagacc acctcggtct acgtcacgca cgaccaggag
     6061 gaggcgatca cgctctccga ccgcatcatc gtgatgagcg agggcgagat cgtgcaggac
     6121 ggcgccccgt ccgagctcta cgaccacccg cggtcgacgt acgtcgccga cttcatgggg
     6181 ttccagaaca tcctccccgg aaccgtcgtc gccgtctccg acggccgggc cgacatccgg
     6241 gtcgacgaca cctcgctggt ggtgaccggc gtggcgacca cggacgtggt cgtcggcgag
     6301 cgggccgacg tcgccttccg cgcagcgcac gtgcgcgtgg accggcaccc ctcggcgtcc
//
LOCUS       DATMSX010000336        10935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_99964,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000336 DATMSX010000000
VERSION     DATMSX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99964"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VN041_10455"
     CDS             complement(<1..402)
                     /locus_tag="VN041_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWU29496.1"
                     /translation="MVAFTKGHGTGNDFVIIEDVDGALELTPSQVQALCDRNFGVGAD
                     GVLRVVRSSAISDGAAALAEEPAAEWFMDYRNADGSIAEMCGNGIRVFAHYLLRSGLA
                     ELEPGSTLPIGTRAGVRDVMRSENGYQVDLGR"
     gene            complement(438..881)
                     /locus_tag="VN041_10460"
     CDS             complement(438..881)
                     /locus_tag="VN041_10460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HWU29497.1"
                     /translation="MTAEIRTFDPADTEDVVALWEEAGLTRPWNDPRADIERMRAVWP
                     DLLIVAVDGDRVVGTVMAGYDGHRGWIYYLASAADRRGEGIGRTLVGEAERRLLALGC
                     PKVQLMVRPGNEIVFAFYDQVGYERFDIGMTGKRLIVDTPTGASA"
     gene            complement(878..1435)
                     /locus_tag="VN041_10465"
     CDS             complement(878..1435)
                     /locus_tag="VN041_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU29498.1"
                     /translation="MTTHYYTASSLDGFIATADHSLDWLLRQDIDEDGPMAYAGFREG
                     LGAMAMGAHTYEWIMRHDEGGWTYTLPTWVFTHRRLDVPDGADIRLTQEDVRAVHGEM
                     VAAAGDKDIWIVGGGELVGRFADAGLIDEVWIQYAPVTLGSGAPVLPRALDLELIDVA
                     RNRNFLCGRYRVTGSLDDAQDGAQA"
     gene            complement(1476..2387)
                     /gene="miaA"
                     /locus_tag="VN041_10470"
     CDS             complement(1476..2387)
                     /gene="miaA"
                     /locus_tag="VN041_10470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWU29499.1"
                     /translation="MTPRVWAVVGATGTGKSELALQLAEALRARGNPAEIVNADAMQL
                     YRGMDIGTAKLSLRERRGIPHHLLDVRDVTEEAAVAWYQPLARASIHAIHEHGGDAIL
                     VGGSGLYVSSVVFDFRFPPRDAAVRARLEAELEEAGTGVLFARLQQQDPGTAERIDPK
                     NGRRIVRALEVLEQGGATHGAALPEKPELWHPETRILGMHTEREILVERLDRRVERMW
                     ADGMLDEVAALRQAGLERGVTASRAIGYAQALAQLRGDVTEQQAVAETQALTRRYARR
                     QVSWFKRYPGIEWLEAGTTAPAGLIDA"
     gene            complement(2506..4059)
                     /gene="miaB"
                     /locus_tag="VN041_10475"
     CDS             complement(2506..4059)
                     /gene="miaB"
                     /locus_tag="VN041_10475"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWU29500.1"
                     /translation="MSLLSSEPTIIAPSAAAIDADGRHRSYEVRTFGCQMNVHDSERL
                     SGSLESAGYVPVAEGEDADIVIINTCAVRDNAAGKLYGTLGHLKSRKDKHEGMQIAVG
                     GCLAQMDQQAVLDKAPWVDVVFGTHNMGSLPGLLERARHNGDAELEILESLEVFPSTL
                     PTKRDSAHSGWVSISVGCNNTCTFCIVPSLRGKEKDRRPGDILSEIRLLVEDGAIEVT
                     LLGQNVNSYGVEFGDRQAFSKLLRAAGEIEGLERIRFTSPHPAAFTDDVIAAMAETPN
                     VMPQLHMPLQSGSDRILKAMRRSYRSSKYLGILDRVRERIPNAAITTDIIVGFPGETE
                     EDFQETMRVVEQSRFSGAFTFQYSIREGTPAATMADQVPKEVVQERYDRLIALQERIS
                     LEENQKQVGREVEVLVSVGEGKKDAETHRLTGRAEDNRLVHFEVTPGSDLPRPGDVVT
                     VTITHGAPFHLLADDPTGAPLRIRRTRGGDAWDRAQAASCGVPAPAGQAAGVPRAVSL
                     GLPTLRVGV"
     gene            complement(4138..4905)
                     /locus_tag="VN041_10480"
     CDS             complement(4138..4905)
                     /locus_tag="VN041_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWU29501.1"
                     /translation="MGHTNGGEEHLAPVIPLFGGARSSKAGAPPPREPDSDSDSDGVP
                     RRTGEEGLWRSALIDGPRRRLDFPAEHSDVEAEAGPDAEEAPVDPEELRRRAEEVLVR
                     RLRGKQLSVSEARGVLRENDVDRETIEDILDEFQHRGYLDDRTLAQLLVTSGVERKGQ
                     GRIALSRALSQRGIPRDVVEEALEELPDDDAERALEFARSKASGMSRLEPDTALRRLL
                     GQLARRGYGGSVAMNAARTALREAGSGRGGVRFVDSD"
     gene            complement(4908..5957)
                     /gene="recA"
                     /locus_tag="VN041_10485"
     CDS             complement(4908..5957)
                     /gene="recA"
                     /locus_tag="VN041_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWU29502.1"
                     /translation="MPSTDDREKALDSALAHIERQFGKGSIMRLGSDERAPVEVISTG
                     SIALDSALGIGGLPRGRIIEIYGPESSGKTTLTLHAIANAQRAGGIAAFIDAEHALDP
                     EYARKLGVDIDQLLVSQPDTGEQALEIADMLIRSGAIDLVVIDSVAALVPEAEIKGEM
                     GDSHVGLQARLMSQALRKLTGGLNQTKTTAIFINQLREKIGVFFGSPETTAGGKALKF
                     YASVRLDIRRIETLKDGAEAVGNRTRVKVVKNKMAPPFKQAEFDILYGIGISREGSLI
                     DFGVEHGIVKKSGSWYTYDGDQLGQGKENARNFLIKNEDIAVAIETQIKQKLGIGVKA
                     ATEAPADELAERRPA"
     gene            complement(6231..6452)
                     /locus_tag="VN041_10490"
     CDS             complement(6231..6452)
                     /locus_tag="VN041_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HWU29503.1"
                     /translation="MRRSEFLRAVDDEFGGRATALTHDLILSGLGRTALEALDAGVPP
                     RDIWLALCEEADVPAERRYGVGRLEPRRR"
     gene            complement(6469..6777)
                     /locus_tag="VN041_10495"
     CDS             complement(6469..6777)
                     /locus_tag="VN041_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29504.1"
                     /translation="MILVRQEIGDVLRDFRLQKGRTLRQVASKASVALGYLSEVERGQ
                     KEASSEILASVADALDVPISTIMREVGDRISVLEGLSSFPDVVPDDLVASVEPELSLH
                     "
     gene            complement(6952..7434)
                     /locus_tag="VN041_10500"
     CDS             complement(6952..7434)
                     /locus_tag="VN041_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWU29505.1"
                     /translation="MSDAALLVAALRERGWTLGVAESLTGGAVAAEIVSVPGASAALW
                     GAVVAYATPVKHTLLGVDAGLLAAHGPVHPDVAVQMAEGVRRAVAVDGRSADVGISTT
                     GIAGPDSPDGQPVGTVHVGVVTPDGTRSGSHLFAGSRAEIRTQARDAAIALALRALRE
                     "
     gene            complement(7431..8006)
                     /gene="pgsA"
                     /locus_tag="VN041_10505"
     CDS             complement(7431..8006)
                     /gene="pgsA"
                     /locus_tag="VN041_10505"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWU29506.1"
                     /translation="MAIPRQLPNAITIARIPLAVVFFVLLLLAGTFGADSLTMRWVAG
                     ALFVLAISTDWVDGYLARRYDIVSDFGKLWDPIADKLLTGAGFLGLAILGEVQWWIVI
                     LILIREWGITVHRFTIVHEHVVAAAWMGKIKTVVQAVALSWALLPLHVWIGMPVWTIT
                     TLVLMIAALVLTVASGIDYVVAQVRGRRDRA"
     gene            complement(8006..9637)
                     /locus_tag="VN041_10510"
                     /pseudo
     CDS             complement(8006..9637)
                     /locus_tag="VN041_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
     gene            9621..9767
                     /locus_tag="VN041_10515"
                     /pseudo
     CDS             9621..9767
                     /locus_tag="VN041_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            10012..10428
                     /gene="rplP"
                     /locus_tag="VN041_10520"
     CDS             10012..10428
                     /gene="rplP"
                     /locus_tag="VN041_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWU29507.1"
                     /translation="MLQPARRKFRKEQKGRNTGIATTGNSVAFGDFGLKCTDRGRLTA
                     RQIEAARRAISRHVKRGGRIWIRVFPDKPISTKPAEVRMGNGKGNPEYYVAEIQPGKI
                     VFEIVGVPEELAREAFRLAAAKLPLRTTFVARQLGA"
     gene            10442..10693
                     /gene="rpmC"
                     /locus_tag="VN041_10525"
     CDS             10442..10693
                     /gene="rpmC"
                     /locus_tag="VN041_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWU29508.1"
                     /translation="MATRKKKETAASAKVTKAATLRTKDVAGLQAEIKDLQKAHFGLR
                     MQKATQQLSNTTTLRVTRRDIARAKTILAQKEQETQAAK"
     gene            10703..>10935
                     /gene="rpsQ"
                     /locus_tag="VN041_10530"
     CDS             10703..>10935
                     /gene="rpsQ"
                     /locus_tag="VN041_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWU29509.1"
                     /translation="MTEAKKSLKRTLIGKVVSDKRAKTVTVLVERRVKHELYGKIVAK
                     TSKYHAHDEKGEYKLGDTIEITESRPISKTKNWV"
BASE COUNT         1726 a         3721 c         3760 g         1728 t
ORIGIN      
        1 gcggcccagg tcgacctgat agccgttctc ggaacgcatc acgtcgcgca caccggcgcg
       61 cgtgccgatc ggcagggtcg agcccggttc gagctcggcc aggccggagc ggagcaggta
      121 gtgggcgaag acgcggatgc cgttgccgca catctcggcg atcgagccgt ccgcgttgcg
      181 gtagtccatg aaccactccg cggcgggctc ctcggcgagg gcggcggcgc cgtcggagat
      241 cgcggaggac cgcaccacgc ggagcacccc gtccgcgccg acgccgaagt tccggtcgca
      301 gagcgcctgc acctggctcg gcgtcagctc gagggcgccg tcgacgtcct cgatgatgac
      361 gaagtcgttg ccggtgccgt gtcccttggt gaatgcgacc atgccaacag tctagagagg
      421 ggcggacatg ccggcgctca ggcggacgcc ccggtcgggg tgtccacgat cagccgcttg
      481 cccgtcatgc cgatgtcgaa ccgctcgtag ccgacctggt cgtagaacgc gaagacgatc
      541 tcgttccccg gacgaaccat gagctgcacc ttcgggcagc cgagcgcgag cagcctgcgc
      601 tcggcctcgc ccaccagcgt gcgtccgatg ccctcaccgc gccgatcggc ggcgctggcc
      661 aggtagtaga tccatccgcg gtgcccgtcg tatccggcca tcacggtgcc gacgacccgg
      721 tctccgtcga cggcgacgat gagcaggtcg ggccagaccg cccgcatccg ctcgatgtcg
      781 gcgcgcgggt cgttccacgg gcgtgtgagg cccgcctcct cccacagcgc gacgacgtcc
      841 tcggtgtcgg ccgggtcgaa ggtcctgatc tctgcagtca tgcctgtgct ccatcctgcg
      901 cgtcgtcgag gctacccgtg acacggtacc gaccgcacag gaagttgcgg ttgcgcgcca
      961 cgtcgatcag ctcgagatcg agggcgcgtg ggagcacggg agcccctgac ccgagtgtga
     1021 ccggcgcgta ctggatccac acctcgtcga tcagcccggc gtccgcgaag cggccgacca
     1081 gctctccccc gccgacgatc cagatgtcct tgtcgccggc ggccgccacc atctcgccgt
     1141 gcaccgcgcg gacatcctcc tgggtgagcc ggatgtcggc gccgtccggc acatcgagcc
     1201 ggcgatgcgt gaacacccag gtcggcagcg tgtaggtcca gccgccctcg tcgtgccgca
     1261 tgatccattc gtaggtgtgc gcacccatcg ccatcgcgcc gaggccttcg cggaaccccg
     1321 cgtaggccat cggaccgtcc tcgtcgatgt cctgtcgcag gagccagtcc agcgagtggt
     1381 cggcggtggc gatgaatccg tccaggctgg aggcggtgta gtagtgggtg gtcatcagtc
     1441 catcgtgacg ggggccaccg acactcacaa cgccgtcagg cgtcgatgag ccccgccggg
     1501 gccgtcgtgc cggcttcgag ccactcgatc cccggatagc gcttgaacca cgacacctgg
     1561 cgtcgggcgt atcgacgcgt gagcgcctgg gtctccgcga cggcctgctg ctcggtgacg
     1621 tcgccgcgca gctgggcgag agcctgcgcg tagccgatgg cacgggaggc ggtgacgccg
     1681 cgctccaggc cggcctgccg gagcgccgcg acctcgtcga gcattccgtc ggcccacatc
     1741 cgctcgacgc gtcggtcgag tcgctcgacg aggatctcgc gttccgtgtg catgcccagg
     1801 atccgggtct ccggatgcca cagctccggc ttctcgggca gtgcggcgcc gtgggttgct
     1861 ccgccctgct cgagcacctc gagtgcccgc acgatgcgac ggccgttctt cgggtcgatc
     1921 cgctccgccg tcccggggtc ctgctgctgc agtcgcgcga acagcacacc cgtgcccgcc
     1981 tcctccagct ccgcctcgag ccgcgcacgc accgcggcgt ccctcggcgg gaagcggaag
     2041 tcgaagacga cgctcgagac gtacagtccg gagccgccga cgaggatcgc gtcgccaccg
     2101 tgctcgtgga tcgcgtggat cgacgcgcgg gcgagcggct gataccaggc gacggcggcc
     2161 tcctcggtca catcgcggac atcgagcaga tgatgcggga tgccgcggcg ctcgcgcagc
     2221 gacagcttgg ccgtgccgat gtccatgccg cggtacagct gcatggcatc cgcgttgacg
     2281 atctcggctg ggttgccgcg cgcgcgcagc gcctcggcca gctggagggc gagttcgctc
     2341 ttccccgtcc ccgtggcgcc cacgaccgcc cagacccgcg gtgtcatgct gatcctcgct
     2401 cagagggccg cacggctccg ccgttctgcg atgctccgca gtcgacggac tcgaggtctg
     2461 cgcacgtgcg cagacctgcg agcgagcgcg acgagaggat gcccgtcaca ccccgacgcg
     2521 cagggtcggc agtccgaggg agacggcgcg cggtactccc gccgcctgtc cggcgggtgc
     2581 gggcacaccg caactcgcgg cctgcgcacg atcccaggcg tcgccgccgc gggtgcgccg
     2641 gatgcgcagc ggagcgccgg tcggatcgtc ggccagcagg tggaacggcg caccatgggt
     2701 gatcgtgacc gtcaccacgt cacccggacg gggcaggtcg gagccgggcg tgacctcgaa
     2761 gtgcaccaga cggttgtcct cggcacgccc cgtgaggcgg tgggtctcgg cgtccttctt
     2821 gccctcaccg acagacacca gcacctcgac ctcgcggccg acctgcttct ggttctcctc
     2881 cagtgagatg cgctcctgaa gggcgatcag acggtcgtag cgctcctgca cgacctcctt
     2941 gggcacctgg tcggccatgg tcgcggcggg cgtgccctcg cggatggagt actggaaggt
     3001 gaaggcaccg gagaagcgcg actgctcgac gacgcgcatg gtctcctgga agtcctcctc
     3061 ggtctcgccg gggaagccga cgatgatgtc ggtggtgatc gccgcgttcg ggatgcgctc
     3121 gcgcacgcgg tcgaggatgc ccaggtactt cgagctgcgg tacgaccgcc gcatggcctt
     3181 gaggatccgg tcgctgccgg actgcaacgg catgtgcagc tggggcatga cgttgggtgt
     3241 ctcggccatc gcggcgatga cgtcgtcggt gaaggcggcc gggtgcgggc tcgtgaagcg
     3301 gatgcgctcc agtccctcga tctcgcccgc ggcgcggagc agcttgctga acgcctgacg
     3361 gtcgccgaac tcgacgccgt aggagttcac gttctggccg agcagggtca cctcgatcgc
     3421 tccgtcctcg accaggaggc ggatctcgct gaggatgtcg ccgggacggc ggtccttctc
     3481 cttgccgcgc aggctcggca cgatgcagaa ggtgcaggtg ttgttgcagc cgaccgagat
     3541 ggacacccac ccgctgtgcg cggagtcgcg cttggtcggg agcgtcgagg ggaacacctc
     3601 gagcgactcg aggatctcca gctccgcgtc gccgttgtgt cgggcccgct cgagcagccc
     3661 ggggagggag cccatgttgt gcgtgccgaa gacgacatcg acccagggag ccttgtcgag
     3721 caccgcctgc tgatccatct gtgccaggca tccgccgacg gcgatctgca tgccctcgtg
     3781 cttgtccttg cgggacttga ggtgcccgag cgtgccgtag agcttgcccg cggcattgtc
     3841 acgcacggcg caggtgttga tgatgacgat gtcggcgtcc tcaccctcgg cgaccggcac
     3901 gtaccccgca ctctccagcg agcccgacag gcgctcggaa tcgtgcacgt tcatctggca
     3961 gccgaaggtg cggacctcat aggagcggtg gcgaccgtcg gcgtcgatcg cggccgcgga
     4021 aggcgcgatg atcgtcggtt cggaggagag aagagacatg atccagccat tctacgatcc
     4081 gccgcggggt gcggcgtcgc cgcagacagc caggccccag cgccctcggc gcacctgtca
     4141 gtcggagtcg acgaaccgca ctccgccgcg gccgctccct gcctcgcgca gcgccgtacg
     4201 cgcggcgttc atcgcgacgg agccgccgta gccgcgcctg gcgagctgac cgagcaggcg
     4261 gcgcagcgcc gtgtccggct ccagtcgtga catccctgat gccttgctgc gcgcgaactc
     4321 gagggcgcgc tcagcgtcgt catcgggaag ctcctcgagc gcctcctcga cgacatcccg
     4381 cgggattcca cgctgcgaca gagcgcggga cagcgcgatc ctgccctgcc cctttcgctc
     4441 gacgccggag gtcaccagca gctgtgcgag ggtgcgatcg tcgagatacc cgcggtgctg
     4501 gaactcgtcg aggatgtcct cgatcgtctc ccggtccaca tcgttctcgc ggagcacgcc
     4561 gcgcgcctcg gacacggaaa gctgcttgcc gcggagccgg cgcaccagca cctcctcggc
     4621 acgacggcgc aactcctcgg ggtcgacggg cgcctcctcg gcgtcggggc ccgcctcggc
     4681 ctcgacgtcc gagtgctcag cagggaagtc caacctgcgt cgcgggccat cgatgagcgc
     4741 ggagcgccag aggccctctt cgcccgtgcg ccgcggcaca ccgtcggagt cggagtcgga
     4801 gtcgggctct cggggcggcg gcgcacccgc cttcgacgat cgcgcgccgc cgaagagagg
     4861 gatgacgggg gcgagatgct cctcgccccc gttcgtgtgc cccatgatca ggccggacga
     4921 cgctcggcga gctcgtcagc cggagcctcg gtcgcggctt tcacgccgat gcccagcttc
     4981 tgcttgatct gcgtctcgat cgcgacggcg atgtcctcgt tcttgatcag gaagttgcgg
     5041 gcgttctcct tgccctggcc gagctggtcg ccgtcgtagg tgtaccagga gccggacttc
     5101 ttgacgatgc cgtgctcgac gccgaagtcg atcaggctgc cctcacggga gatgccgatg
     5161 ccgtacagga tgtcgaactc ggcctgcttg aacggcgggg ccatcttgtt cttgacgacc
     5221 ttgacgcggg tgcggttacc gacggcctcg gcaccgtcct tgagggtctc gatgcggcgg
     5281 atgtccagac gcaccgacgc gtagaacttc agcgccttac caccggcggt ggtctcgggc
     5341 gagccgaaga agacgccgat cttctcgcgc agctggttga tgaagatggc agtggtcttg
     5401 gtctggttca gcccaccggt cagctttcgg agagcctgcg acatcagtcg agcctggagg
     5461 ccgacgtgcg agtcgcccat ctcacccttg atctcggcct cggggacgag ggcggcgacg
     5521 gagtcgatga cgaccaggtc gatcgcgccg gagcggatca gcatgtcggc gatctccagc
     5581 gcctgctcac ccgtgtcggg ctgagagacc agcagctggt cgatgtcgac gccgagcttg
     5641 cgtgcgtact cggggtcgag tgcgtgctcc gcgtcgatga acgcggcgat gccgccggcg
     5701 cgctgggcgt tggcgatggc gtgcagcgtg agcgtggtct taccggagga ctcggggccg
     5761 tagatctcga tgatgcggcc gcgcgggagc ccgccgatgc cgagtgcgga gtccagggcg
     5821 atcgagccgg tggagatgac ctcgacgggg gcgcgctcgt cgctgcccag tcgcatgatc
     5881 gatcccttgc cgaactgacg ctcgatgtgc gcgagtgcgg aatccagtgc cttctcgcgg
     5941 tcgtcggtgg atggcatgac gtgctcctca gtgtctcgaa tgctgccgcc tgtaggctgt
     6001 cgcggcctct ccgggatgga tcgactctgc gacaaggcgc atggctggtg ccacatccat
     6061 cacgctagag ggggcctccg acatcgagtc gcgagagccg ccttctgtgg atgaagatca
     6121 caccaggacc acttgtgcag aacgatacgc gcatctcgaa cacatcttcg acgacacgcc
     6181 gcacggtcgc tctcgacttt cgatcgccgc tcgcgctcag gccccgcggc tcaacgccgt
     6241 cgcggctcca gccggcccac gccgtaacga cgctcggcag gcacgtcggc ctcctcgcac
     6301 agggcgagcc agatgtcccg cggcgggacg cccgcgtcca gcgcctccag cgccgttctg
     6361 ccgagcccgc tgaggatcag gtcgtgcgtg agggccgtcg cgcgcccgcc gaactcgtcg
     6421 tcgacagcgc gcaggaattc actgcgtcgc atccgtgcct ctggtcgatc agtgcagcga
     6481 gagctcgggc tcgaccgagg cgaccaggtc gtccggcacg acgtccggga agctgctcag
     6541 gccctcgagg accgagatgc ggtcaccgac ctcgcgcatg atcgtcgaga tggggacgtc
     6601 gagtgcgtcg gctaccgagg cgaggatctc gctggaagcc tccttctgac cgcgctcgac
     6661 ctcgctcagg tatccgagcg cgaccgatgc cttgctcgcc acctgtcgga gggtgcgccc
     6721 cttctgcagg cggaagtccc gcagcacatc gccgatctcc tgtctgacaa ggatcatcgg
     6781 aacctcctct ccacgatccc cctcggggtc cccgagtgga tggccagtct aacccgactg
     6841 gatgatggtg aatctactgt ttctcgctgt ggttctgttg tgagggatga tgcgggtgtg
     6901 acggtgtgtc gattgctgag cacatcaggg acaacccgct tccggcatcc gctattcccg
     6961 cagcgcgcgc agcgccagcg cgatcgcggc gtcccgcgcc tgcgtgcgga tctccgcgcg
     7021 cgatcctgcg aagaggtggg atccggaacg agttccgtcc ggcgtgacca cgccgacgtg
     7081 aacggtcccg accggctgcc cgtcaggcga gtcggggccg gcgatgcccg tggtggagat
     7141 gccgacatcc gcagacctgc cgtcgaccgc caccgcgcgg cgcacgccct cggccatctg
     7201 cacggcgaca tcggggtgaa ccgggccatg cgcggcgagc agaccggcgt ccacgcccag
     7261 cagcgtgtgc ttgacgggtg tcgcgtaggc gaccaccgct ccccagagcg ctgcggatgc
     7321 cccaggaacc gacacgatct cggcggcgac cgctcccccg gtcagcgact ccgcgacgcc
     7381 cagcgtccat ccgcgctcgc gaagggctgc gacgagaaga gcggcgtcgc tcatgcccgg
     7441 tcccggcgac cgcgcacctg cgcgaccacg tagtcgatgc cgctcgcgac cgtgagcacg
     7501 agtgccgcga tcatgagcac gagggtcgtt atcgtccaga ccggcatccc gatccacacg
     7561 tgcagcggca gcagcgccca gctgagcgcg acggcctgga ccacggtctt gatcttgccc
     7621 atccaggctg cggcgacgac atgctcgtgc acgatcgtga agcggtggac ggtgatcccc
     7681 cactcccgga tcaggatgag gatcacgatc caccactgca cctcgccgag gatcgccagg
     7741 cccaggaacc ccgcaccggt gaggagcttg tcggcgatcg gatcccacag cttgccgaag
     7801 tcgctgacga tgtcgtaccg gcgggcgaga tagccgtcca cccagtcggt ggagatggcc
     7861 agcacgaaca gcgcgccggc gacccagcgc atcgtgaggc tgtccgctcc gaaggtgccc
     7921 gccaggagca ggagcacgaa gaacacgacc gccagcggga tgcgggcgat cgtgatcgcg
     7981 ttgggcagct gccgagggat cgccatcagt cgcggcccgt caggccccag gcgtcctcgt
     8041 caccgtcgtc ctcgctctcg acgacgggga ggtcccggaa ctgctgctcg accgggtcct
     8101 gcgcgtacat gtcgtgctcg gccggcgcgg gggcgggtgc cgcaggtgcg gccgcgggtg
     8161 cggagggcgt gtcgccgcgg agcttggcca gcaccgcggg cagctgctcg ttggtgacga
     8221 gcacgtcccg ggccttggag ccctcggacg ggccgacgat ctcgcgggac tccagcaggt
     8281 ccatcaggcg gccggccttg gcgaagccga cgcgcagctt gcgctgcagc atcgaggtcg
     8341 agccgaactg cgaggacacg atcagctctg ctgcggccag cagcagttcg aggtcgtcgc
     8401 cgatgtcctc gtcgacctcc ttcttcgtcg ccgccgcctc ctgcacgtcc gcgcgatact
     8461 cggggcgggc ctgttgcgtg acgtgcttga cgaccgtcgc gatctcggcc tcgtccaccc
     8521 aggcgccctg gaggcggaag ggcttggagg agcccatcgg cgagaacagg gcgtcaccct
     8581 gtccgatgag cttgtcggca cccggggcgt ccaggatgac gcggctgtcg gtgacgctgg
     8641 tgacggcgaa ggccagacgc gagggcacgt tggccttgat caggccggtg acgacgtcga
     8701 cgctgggtcg ctgcgtggcg agcacgaggt ggattccgga ggcgcgcgcc agctgggtga
     8761 tgcgcacgat cgagtcctcg acgtcgcgcg gggcgaccat catcaggtcg gcgagctcgt
     8821 cgacgacgac cagcaggtac gggtacggct tgagcacgcg ctcactgccg acggggacct
     8881 cgacctcgcc ggcgcggacc gcgcggttga agtcgtcgat gtgacggaac ccgaacgacg
     8941 cgaggtcgtc gtagcgcatg tccatctcct tcacgaccca ttgcagcgcc tcagcggcct
     9001 tcttggggtt cgtgatgatg ggggtgatca ggtgcggcac acccgcgtag gacgtgagct
     9061 ccacgcgctt cgggtcgatg agcaccatgc gcacgtcggc gggacgagcg cgcatcagca
     9121 ggctcgtgat catggagttc acgaagctgg acttacccga gcccgtggag ccggccacca
     9181 ggaggtgagg catcttggcg aggttggcga cgacgaactt gccgccgacg tccttgccga
     9241 cgccgatggt cagcgggtgc gtgctcttct gcgcggcggc tgagcgcagc acgtcgccca
     9301 gcgcgacggt ctccttgtcg acgttcggga tctcgatgcc gatggcgctc ttgccaggga
     9361 tcggcgagag gatgcgcacg tcgttggagg cgaccgcgta ggcgaagttg ttgctcagct
     9421 gcaggatctt ctcgaccttg acgcccagac ccacctcgac ctcgtactgc gtgaccgtcg
     9481 ggccgcgcga gaagccggtg accttggcgt cgaccttgaa ctgctcgagc acgctggtga
     9541 tctgggcgat cgtctggtcg gtggcatccg atcgggtgac cggcggcggc ccggccttga
     9601 ggatgcccgg gttgggaagc acgtacggcg ccgcaggcgt caaggtctgg gtctacaagg
     9661 gcgacacgct gggtcgtaac gacctgcccg ccgtcgaaac gccgcgtccg gacgaagagc
     9721 gccgcccgcg tggtccccgc cgtgatggtc gccctggcga ccgtccgggt ggcgaccgcc
     9781 gtggtcccgg cccccgtgcc ggtgcccgtg gtccgatcgg tggcaacacc gctccggccg
     9841 atggcagcga caagcccgca gaagcaaccg gcggtgccgg tgcagactcg aaacccgccg
     9901 ttaagcgcgt ccgcaaagcc gcgcccgctg cagcagcgga cggtgccaag accgagtaat
     9961 cggtcgctcc cgggcacgca agtgcctggg gccctcacgg agtaacaaaa catgctgcaa
    10021 cctgcacgca gaaagttccg caaggaacaa aagggccgca acaccggcat cgcaaccacg
    10081 ggtaactcgg ttgcgtttgg tgatttcggt ctcaaatgca ccgaccgcgg ccgcctcacg
    10141 gcgcgccaga tcgaagccgc tcgtcgcgcg atttcgcgtc acgtgaagcg cggtggccgt
    10201 atctggatcc gcgtgttccc ggacaagcca atctctacca agcccgctga agtgcggatg
    10261 ggtaacggta agggcaaccc cgagtactac gtcgctgaaa tccagcctgg caagatcgtg
    10321 ttcgagatcg tcggcgtgcc cgaagaactc gcccgcgaag cgttccgcct ggccgccgcc
    10381 aagcttccgc tgcgtacgac gttcgtcgct cgccagctcg gcgcctgaga ggagaacacc
    10441 catggcaacc cgtaagaaga aggaaaccgc ggcttcggcc aaggtgacga aggctgcaac
    10501 cctgcgcacg aaggacgtcg caggcctgca agccgaaatc aaggatctgc agaaggccca
    10561 tttcggcctg cgcatgcaga aggccacgca gcagctgtcg aacaccacga cgctgcgcgt
    10621 gacgcgtcgc gacatcgcgc gcgcaaagac cattcttgct cagaaagagc aagagaccca
    10681 agccgccaag taaggagtga acatgacgga agctaaaaaa tccctcaagc gcaccttgat
    10741 cggcaaggtg gtcagcgaca agcgtgccaa gaccgtgacc gtgctggtcg agcgccgtgt
    10801 gaagcacgag ctctacggca agatcgtggc caagacgagc aagtaccacg cccatgacga
    10861 aaagggcgag tacaagctgg gcgacaccat cgagatcacg gaaagccgtc cgatctcgaa
    10921 gaccaagaac tgggt
//
LOCUS       DATMSX010000337         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_43344,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000337 DATMSX010000000
VERSION     DATMSX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43344"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VN041_10535"
     rRNA            complement(<1..1101)
                     /locus_tag="VN041_10535"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          532 a          486 c          395 g          614 t
ORIGIN      
        1 acaggggttg cgctcgttgc aggacttaac ctaacacctc acggcacgag ctgacgacaa
       61 ccatgcagca ccttgaaaat tgcccgaagg aaggtctatt tctaaaccga tcaattccca
      121 tttaagcctt ggtaaggttc ctcgcgtatc atcgaattaa accacataat ccaccgcttg
      181 tgcgggcccc cgtcaattcc tttgagtttc attcttgcga acgtactccc caggtggcta
      241 acttatcact ttcgcttagt ctctgaatcc gaaaacccaa aaacgagtta gcatcgttta
      301 cagcgtggac taccagggta tctaatcctg ttcgctcccc acgctttcgt ccatcagcgt
      361 cagttaagac atagtgacct gccttcgcaa ttggtgttct aagtaatatc tatgcatttc
      421 accgctacac tacttattcc agccacttct accttactca agacctgcag tatcaatggc
      481 agtttcacag ttaagctgtg agatttcacc actgacttac agatccgcct acggaccctt
      541 taaacccaat aaatccggat aacgcttgca ccctccgtat taccgcggct gctggcacgg
      601 agttagccgg tgcttattcg tacagtacct tcagctattt acacgtaaat aggtttatcc
      661 ctgtacaaaa gaagtttaca acccataggg ccgtcgtcct tcacgcggga tggctggatc
      721 aggcgctaac ccattgtcca atattcctca ctgctgcctc ccgtaggagt ctggtccgtg
      781 tctcagtacc agtgtggggg atcaccctct caggccccct aaagatcatc gacttggtga
      841 gccgttacct caccaactat ctaatcttgc gcgtgcccat ctttatccac ctcagttttc
      901 aatataaagt gatgccactc tatatattat ggggtattaa tcttcctttc gaaaggctat
      961 ccccctgata aaggcaggtt gcacacgtgt tccgcacccg tacgccgctc tcaagtctcc
     1021 gaagaaactc taccgctcag cttgcatgtg ttaggcctcc cgctagcgtt catcctgagc
     1081 caggatcaaa ctctccattg tatgtttgtc ttgactcact caaagttttt taacgcttta
     1141 gtttttcctt acttggttgt tatattgtat gtcaatgatc ttcatttctt ccgcttccta
     1201 caaactaatt cttctgtcag ttttcgtttc gtatttgcga gtgcaaaagt aaaaaatatt
     1261 tctgaattga ccaaatgttt ttgaagaaaa ttttaaagtt tttttagtaa ccttaaaccc
     1321 tcttaaacga tccctcaaat caactcctgc tccccgtttt accggactgc aaagatacaa
     1381 atctttttta ttcccacaag atttttttta attaaatttt gtaaaagttt ttttcgtatc
     1441 cccttttcag agcttctatt ttttaaaccc cgaaaggcat ctcctgcgct acctacttcc
     1501 ttccgttttc agtggggcaa agataacaac actttatcct atattccaaa tctatttaac
     1561 ataaaattta agtttacgca atttttatcc ttaactctct ggttgtatgc atgaataatt
     1621 ttaagcctgt aaggggagaa aggaagaagg gagatgggag atgggaggct gcgaaggaga
     1681 gagagtgggt ggatctatat atatgtatgt gaaggttggg tgttgggggt tgggtgccca
     1741 tgataggagc aggggttggt caaagagaag ataagctaac ttataagctg gaaaaagtaa
     1801 aaagtgaaaa gtgagaggtg gggagtgatg gatgaaggac aggcaggata taaggggatg
     1861 ttctctaagt tacctaccgc agttttgaat tatgaaggtt ggaaatttga aaggatgaac
     1921 gtaatcataa atcctaaacc aaaactttga atattccatt cctaaacttt ctcctctcac
     1981 ttcttacttc ttacttctta ctttaacccc caccctagcc cggatcg
//
LOCUS       DATMSX010000338         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_14567,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000338 DATMSX010000000
VERSION     DATMSX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14567"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1433
                     /locus_tag="VN041_10540"
     CDS             153..1433
                     /locus_tag="VN041_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29510.1"
                     /translation="MMSSTSTTPSAVSPDREVPGSPQPRVFIPLFVLSWFGTNLALST
                     IGGAAIPKAFAFLDDSTKEINLSIATLIGGVVVMIVTPLFGRLSDRTRSRLGIRRPWI
                     LGGAIAGLIGAIVLGFAQSLWPIALGWAIVQIGFGATNAAVHALLADQIPARIRARVS
                     GIASASASIGLILGTQIIAALPNDAQWLWFVVPGVIGAVLSALLFLGLDDIVRVEPRP
                     AWSWRDVLSTYWLSPRSHPDFFWAWTCRLLVTMSVFSVATYLLFFIIDRLGIPKETAS
                     GVQALALLVFTVGGLITNILFGWISDRTGRRKPVIWISCAFSAGGLLLAMFAPDIPMF
                     LAAIALVGAAQGAYVSVDIALMTEVLPHFRDAGKDLGIVALSYQVPQFLVPMLAIPLL
                     AIGGDGPNYTALFAAAIVFGVLGGLAVLPIRSVR"
     gene            1700..2167
                     /locus_tag="VN041_10545"
     CDS             1700..2167
                     /locus_tag="VN041_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU29511.1"
                     /translation="MADLDDTDRRILAVLDSDPRRPVALIAQDLSLARGTVHARLARL
                     ESSGWLRPNSSRVLPSALGRGVSASIQVELDQHQIGAAIEALSRIPEVLECFAPAGDT
                     DLLLRVVATDPDDLYRVAEVVRLCPGILRTSTSLFLRQVIPYRVSELLKAPLP"
BASE COUNT          294 a          847 c          736 g          394 t
ORIGIN      
        1 cggtgacggt gcacgtcggc gccggccacg tgcacggctc gcccggtctc accgccggct
       61 ggcagggagc acgcgactgg cagtccgcct tcgccgtcga actgacccgc gcgtaccacc
      121 gttgagcccg ccccgcccac cctgaaagga cgatgatgtc ttccacctcc accaccccct
      181 cagcggtctc gccggaccgc gaggttcccg gctccccgca gccgagggtc ttcatcccgc
      241 tgttcgtgct gagctggttc ggcaccaacc tcgccctcag caccatcggc ggagccgcga
      301 tcccgaaggc gttcgccttc ctggacgatt cgaccaagga gatcaacctc tcgatcgcga
      361 cgctgatcgg cggcgtcgtg gtgatgatcg tcaccccgct gttcggccgg ctgagcgacc
      421 gcacccgctc acgcctcggc atccggcgcc cctggatcct cggcggagcg atcgccgggc
      481 tcatcggtgc gatcgtgctc ggcttcgcgc agtcgctctg gccgatcgcg ctcggctggg
      541 cgatcgtgca gatcggcttc ggagcgacca acgccgccgt gcacgccctg ctcgcggatc
      601 agatccctgc ccgcatccgc gcccgcgtct cgggaatcgc gagcgcgtcc gccagcatcg
      661 gcctgatcct cggcacgcag atcatcgccg cgctgcccaa cgacgcgcag tggctgtggt
      721 tcgtcgtgcc gggcgtgatc ggagcggtgc tgtcggctct cctgttcctc ggactggatg
      781 acatcgtgcg cgtcgagccc cggccggcgt ggagctggcg ggacgtgctg tcgacgtact
      841 ggctgagccc gcgcagccac cctgacttct tctgggcgtg gacctgccgg ctgctggtga
      901 cgatgtcggt gttctcggtg gccacctacc tgctgttctt catcatcgac cggctcggca
      961 tccccaagga gacggcctcc ggagtgcagg cgctggcgct gctcgtgttc accgtcggcg
     1021 ggctgatcac caacatcctc ttcggctgga tctccgaccg caccggcagg cgcaagccgg
     1081 tgatctggat ctcctgcgcc ttctcggccg gcgggctgct gctcgcgatg ttcgcgccgg
     1141 acatcccgat gttcctcgcc gcgatcgcgc tggtgggcgc ggcccagggc gcctacgtct
     1201 cggtcgacat cgcgctgatg acggaggtgc tcccccactt ccgggatgcc gggaaggatc
     1261 tcggcatcgt cgcgctgtcg taccaggtac cgcagttcct ggtgccgatg ctggccatcc
     1321 cgctgctggc catcggcggc gacgggccga actacaccgc gctgttcgcc gcggccatcg
     1381 tcttcggggt gctcggcggg ctcgccgtgc tgccgatccg ctccgtgcgc tgagtcgcgc
     1441 gcgggatccg cggccgagac gggacgagcg ctgccgctga gattcacgcg gcagcgctcc
     1501 cgggattccg cccgatcgtc gaccccctcc gcacggctcg tgaccgtcct ctgagcgcct
     1561 gccgatccgc actcgtgatg ccgatcggtc atttgactca ctgatctcag cacacagcgg
     1621 tctgcaagca ccataggatc gattgcgatt gatcgatccg accgatcaga cccgccgaca
     1681 ggaagcagtg gtgatcgata tggccgacct cgacgacacc gaccggcgca tcctcgcggt
     1741 gctcgactcc gacccgagac gcccggtggc cctgatcgca caggacctga gtctcgccag
     1801 gggcaccgtg catgcgcggc tggctcggct ggagtcgagc ggatggctgc gcccgaacag
     1861 ctcccgcgtg ctgccgtcgg cgctcggccg cggcgtctcg gcgtcgatcc aggtggagct
     1921 cgaccagcac cagatcggcg cggccatcga agctctcagc cgcattcccg aggtgctgga
     1981 gtgcttcgcc cccgcgggcg acaccgacct gctgctgcgc gtggtcgcca ccgacccgga
     2041 cgacctctac cgcgtcgccg aggtggtgcg gctgtgccct ggcatcctgc gcacctcgac
     2101 gagcctgttc ctgcgccagg tcatccctta ccgcgtcagc gaactgctga aggcgcccct
     2161 gccctgagcc cgtctcggcc ccaggcgggc tcagccgatg atctgagcac cgggcaccgg
     2221 gccgtgcacg tgcagcgcct cgcgccccgt ggccttcagc tcgtcctgca c
//
LOCUS       DATMSX010000339         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_43362,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000339 DATMSX010000000
VERSION     DATMSX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43362"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1033)
                     /locus_tag="VN041_10550"
     CDS             complement(131..1033)
                     /locus_tag="VN041_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29512.1"
                     /translation="MDADAVPHDTGQQEWSPPLPPAPGFAHRMVRTPGARCHVAEIGE
                     GDPVLLLHGFPEHWWQWRLIAPRIAAHGYRVICPDLRGSGWTETDAPGMHRTIQRDDV
                     LAILDALGVGRVHVMSHDMGAISAMQLAYTHPDRIRSMVQLSVPPGFLEFTPRIVPAF
                     AHMPALLMHRPPRSMGYLFAAPYISHPLSADVLDGYLAPERRPEVLPNVRALYRGMVI
                     PEVVRLVRGDYKRMRLQPPTLFVFGREDRPFSEANVRRISRHHDRCAERFELAFVDDA
                     AHFITDDAPDAVVDLALEWFAREG"
     gene            1141..1677
                     /locus_tag="VN041_10555"
     CDS             1141..1677
                     /locus_tag="VN041_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU29513.1"
                     /translation="MSTTRRRALEAAVTLIGTEGIRALTHGRVDAAAGLPAGSTSNHF
                     RTRAALVAGVAEWIARREMGDAGTPEITTRDEFVGALTRMIEVQSGPLAARTRARYAL
                     FLEADDEAVRPLREQRTGMEAWVRSILTRLGGDAAARNTTFLMAAGDGLLLHRLTVDP
                     DAPIDDVVSHAIDAALQL"
     gene            1736..2431
                     /locus_tag="VN041_10560"
     CDS             1736..2431
                     /locus_tag="VN041_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU29514.1"
                     /translation="MTDARILVVDDEPNIRDLLSRGLSFAGFKVKTVANGAATISAVL
                     EEEPDLIILDVMLPDMNGFSVTKRLRGAGFTAPILFLTAKDDTQDKIEGLNAGGDDYV
                     TKPFALDEIVARAQAILRRTMQNGDEESLIRAGELSMDQDTHDVFVGKVPIELSPTEF
                     KLLKYLMLNPNRVLSKAQILDHVWEYDFNGDAGIVESYISYLRRKIDPHTEESVIQTK
                     RGFGYMLKVGKTA"
     gene            2454..>3605
                     /locus_tag="VN041_10565"
     CDS             2454..>3605
                     /locus_tag="VN041_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU29515.1"
                     /translation="MAQGADSVTQWWRRISLRAKVTGVTVAVLALGLVVAGIGTVPIL
                     RGAMINNINQQLPSLTGTGQAERFFDVSIPDGKLVFDQKDDAPRATDFVVAFYTTAGD
                     PIATAGGQSTPEHRPQFPAKLSLQSAQAMGDTTVTLTTPSGQTYTAAAATQSLQKIAG
                     VTYVQMVALPLEEADKIVGTYFAVYTTVALVTILLAALLIRGLVTLTFRRLGHVESTA
                     MAIAAGDFSQRLTDLEPTTEVGRLNSAINTMLERVDQSLAQRDRTVQNMRRFIGDASH
                     ELRTPLVSVRGYAELYRMGAISGDEDTARAMERIEKEAIRMGVLVEDLLALARLDEDR
                     EKQLEIVPIDLRPVARDAALDLRVASPGRTITVIDNTAEEQEPPRLHPAA"
BASE COUNT          619 a         1275 c         1205 g          506 t
ORIGIN      
        1 gcgggcgcgg tgcaggcggc ggtggcggcg ggtacgcggc cggcgatgca tgggcggtgc
       61 gggtatcgat gtcggacggc acacgacgat ccttgcggat ccgagcagtc cgcgcatcgc
      121 cccgcgccgt tcagccctcg cgcgcgaacc actccagcgc cagatcgacg acggcatccg
      181 gtgcgtcgtc cgtgatgaag tgcgccgcat cgtcgacgaa ggcgagctcg aatcgctcgg
      241 cgcagcggtc gtgatggcgg ctgatccgcc gcacgttcgc ctcgctgaag gggcgatcct
      301 cccggccgaa gacgaacagc gtcggcggct gcagccgcat gcgcttgtaa tccccgcgca
      361 ccagcctgac cacctcgggg atgaccatgc cccggtacag cgcgcgcacg ttcggcagca
      421 cctcggggcg ccgctccggc gcgagatagc cgtccagcac gtctgccgac agcgggtggg
      481 agatgtacgg agcggcgaag aggtatccca tcgatcgcgg cggacgatgc atcaacaggg
      541 ccggcatgtg cgcgaaggcg gggacgatcc ggggcgtgaa ctccaggaac cccggaggca
      601 ccgacagctg gaccatcgag cggatgcggt cgggatgcgt ataggcgagc tgcatcgcgg
      661 agatcgcacc catgtcgtgg ctcatcacat gcacgcgccc gacgcccagc gcgtcgagga
      721 tcgccagtac gtcgtccctc tggatcgtcc ggtgcatgcc gggggcgtcg gtctcggtcc
      781 atccggatcc gcgcaggtcg gggcagatca cccgatagcc gtgagccgcg atccgcggcg
      841 cgatgagccg ccactgccac cagtgctcgg ggaagccgtg cagaagcagg acggggtcgc
      901 cctcgccgat ctcggcgaca tggcaccgcg ctccaggggt gcggaccatc cggtgcgcga
      961 agcccggcgc cggcggcagc gggggcgacc actcctgctg cccggtgtca tgaggaactg
     1021 cgtctgcgtc catgagaggg cctccacgtc gctgtgcgga ccgggccttc cggccatgcc
     1081 ctcaccttac tatgttcata gtgaattgtc actatgaaca tagttgcgta ggctcggcat
     1141 gtgagcacca cgcgacgacg cgcactggaa gccgccgtga ccctgatcgg caccgaaggc
     1201 atccgcgcgc tgacacacgg ccgagtggat gccgcggcag gcctccccgc agggtcgacc
     1261 tcgaaccact tccgcacccg cgcggcgctg gtggcgggcg tggccgaatg gatcgcccgg
     1321 cgcgaaatgg gtgacgccgg cactccggag atcaccacgc gcgacgagtt cgtcggcgcg
     1381 ctcacccgga tgatcgaggt gcagtccggc ccgctggccg cgcgaacccg cgcccggtac
     1441 gcgctcttcc tcgaagcgga cgacgaggca gtgcgcccgt tgcgcgagca gcggacgggc
     1501 atggaggcgt gggtgcgcag catcctgacc cgtctcggcg gcgacgctgc cgcgcgcaac
     1561 acgaccttcc tcatggccgc cggtgacggg ctgctgctgc atcgcctcac cgtcgacccg
     1621 gacgccccga tcgatgacgt ggtctcccac gcgatcgacg ccgcgctgca gctctgaccg
     1681 ccgcatctcc gaacgcaatc agcccgcgca ggggcggagt caccataatg gggtcatgac
     1741 tgatgcgcgc atcctggtcg tcgacgacga accgaacatc cgcgacctgc tctcccgggg
     1801 tctcagcttc gccgggttca aggtgaagac cgtcgccaac ggcgcggcga ccatctccgc
     1861 cgtcctcgag gaggagccgg acctcatcat cctcgacgtg atgctgccgg acatgaacgg
     1921 cttcagcgtc accaagcgac tgcgcggggc gggcttcacc gccccgatcc tgttcctcac
     1981 cgccaaggac gacacccagg acaagatcga gggcctgaac gccggtggcg acgactacgt
     2041 caccaagccc ttcgccctgg acgagatcgt cgcgcgcgcc caggccatcc tgcgccgcac
     2101 gatgcagaac ggcgacgagg agtcgctgat ccgcgccggc gagctgtcga tggatcagga
     2161 cacccacgac gtcttcgtgg gcaaggtgcc gatcgagctg agccccaccg agttcaagct
     2221 gctgaagtac ctcatgctca accccaaccg ggtgctctcc aaggcgcaga tcctggacca
     2281 cgtgtgggag tacgacttca acggcgatgc gggcatcgtg gagagctaca tctcgtacct
     2341 gcgccgcaag atcgacccgc acaccgagga gtcggtcatc cagaccaagc gcggcttcgg
     2401 ctacatgctc aaggtcggca agaccgcctg agcgcgggct tcagagcact cccatggcac
     2461 aaggcgcgga ctccgtcacc cagtggtggc ggcggatcag cctgcgcgcc aaggtcacgg
     2521 gagtgaccgt cgcggtgctc gccctcgggc tcgtcgtcgc ggggatcggc accgtgccga
     2581 tcctgcgcgg tgcgatgatc aacaacatca accagcagct gccctcactg accggcaccg
     2641 ggcaggcgga gcgcttcttc gacgtgtcga tccccgatgg gaagctggtc ttcgaccaga
     2701 aggacgacgc gccccgcgcg acggacttcg tggtcgcctt ctacacgaca gcgggagatc
     2761 cgatcgcgac cgccgggggg cagagcaccc ccgagcatcg cccgcagttc cccgcgaagc
     2821 tcagcctgca gtccgcgcag gccatgggcg acacgacggt cacgctcacc actccgagcg
     2881 gccagacgta cacggcagcg gcagccaccc agtccctgca gaagatcgcc ggcgtcacct
     2941 acgtgcagat ggtcgcactg cccctggagg aggccgacaa gatcgtcggc acctacttcg
     3001 cggtgtacac gaccgtggcg ctggtgacga tcctgctcgc ggcgctgctg atccgcggtc
     3061 tcgtcacgct cacgttccgc agactcggcc acgtcgagtc caccgccatg gccatcgccg
     3121 ccggcgactt cagccagcgg ctcacggatc tggagccgac caccgaggtc ggacgcctca
     3181 actccgccat caacaccatg ctggaacgcg tcgaccagtc gctcgcccag cgcgaccgca
     3241 cggtgcagaa catgcggcga ttcatcggcg atgccagcca tgagctgcgc actccgctgg
     3301 tgagtgtgcg gggatacgcc gagctgtacc ggatgggcgc gatcagcggc gacgaggaca
     3361 ccgcacgcgc catggagcgc atcgagaagg aggcgatccg gatgggcgtg ctcgtcgagg
     3421 acctcctggc cctcgcccgc ctggacgagg accgcgagaa gcagctcgag atcgtcccga
     3481 tcgacctgcg gccggtcgcc cgcgacgccg ccctggacct gcgcgtcgcc tcgccggggc
     3541 gcacgatcac cgtgatcgac aacaccgccg aggaacagga gcccccgcgc ctgcatccgg
     3601 ccgcc
//
LOCUS       DATMSX010000340        13530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_33637,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000340 DATMSX010000000
VERSION     DATMSX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33637"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2286
                     /gene="secA"
                     /locus_tag="VN041_10570"
     CDS             <1..2286
                     /gene="secA"
                     /locus_tag="VN041_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWU29516.1"
                     /translation="GQTPAVRREQYAADITYGTNNEFGFDYLRDNMAWRKEDLVQREH
                     FFAIVDEVDSILIDEARTPLIISGPSSGEANRWFTEFAKIARTLEPGEDYEVDEKKRT
                     IGVLEPGIEKVEDYLGIDNLYESANTPLISFLNNSIKAISLFKRDTDYVVMNDEVMIV
                     DEHTGRILVGRRYNEGIHQAIEAKENVPVKAENQTLATVTLQNYFRLYDKLAGMTGTA
                     ETEAAEFMSTYKLGVVPIPTNKPMIRKDQPDLVYKNEQAKFAQVVEDIAERHAAGQPV
                     LVGTVSVEKSEYLSRLLAKRGVKHEVLNAKNHAREAEIVALAGKLGAVTVATNMAGRG
                     TDIMLGGNAEFLAVQELKSKGLDPEETPEEYEAAWDETYEAMKKKVAEDSVKVTEAGG
                     LYVLGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLTDDLMRLFQSGAAEAILARTN
                     FPDDVPIESGLVSRAIRSAQSQVEARNAEMRKNVLKYDDVLNRQREAIYADRRQILHG
                     DDIADRVKHFIEDAIGGVVDDHTGEGHNESWDFDALWTELKTLYPMNVTIDEVVAEAG
                     GRKGGISAEGLKRELLSDALIAYQKREEALGEAATRELERRVVLQVLDRRWRDHLYEM
                     DYLKDGIGLRAMAQRDPLIEYQREGYAMFQSMMGQIKEESVGYLYNLEVEVRRAGADQ
                     TEVEAKGLVDQQPEQKLEYSAPNDAGDVEVRNERGQVQQAATERAATAQRGAFGQQVD
                     AEEQPAPANREQRRAQSKKKK"
     gene            complement(2425..4062)
                     /locus_tag="VN041_10575"
     CDS             complement(2425..4062)
                     /locus_tag="VN041_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29517.1"
                     /translation="MTAPAPASVSTAGQLKWWKKGDIAAFFALFTNNLTNIITFTALL
                     TMAGLPVDMVVGRIAPAFGLAILLTSSMYVWFAKRLAAKEGRDDVVALPSGPSAPSIF
                     TVTFLVILPVYSTTQDAELAVGIGLVWGFLEGLILFVGSFFGDLLRRAVPRSVLLACL
                     AGLGLLLLAMNPMLQSFQFPVVAFVVLTLVLMNWFGRSPFLGKVPTGLLLLIVGTAAA
                     WAFGLQTPEAVQAALVHAGWHPPQVSITNFIDGVAHAGPFLASAVPLALANYVFDLEN
                     IEAAEAAGDHYSARPVMLVNGGATMVGALMGNPYPVTVYIGHTAYKEMGAGIGYTMLN
                     GITMFAVGLFGMSALLLSVVPMAAIAPILIYIGVVTAAQSVRETPKVELPVIFIALFP
                     WIANWANSLINNTLKAAGTNTAEVGADALNHAGTYFAGISTLGNGAPLSSLLWGMIAI
                     FAIRNKPLQGAIIGALAGVLSFFGVIHAHVPGLPEKFALSDTHVMFLVAYLMVAALFL
                     AKWIQDRITNESVTFTANDEITLSNAVAPSLIQSSKG"
     gene            complement(4059..5483)
                     /locus_tag="VN041_10580"
     CDS             complement(4059..5483)
                     /locus_tag="VN041_10580"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HWU29518.1"
                     /translation="MSVNDLFSSELKPVNLGLDMFADDLKTQGVEPVLMDWTPPGGGD
                     PEVIAALSRLEDPAVAEKIDAANEVALERILSSQPFLEGFGQAIDTVPGMTAKTILHA
                     GPPIEFTRMSGPMKGAVTGALVFEGLAKDVDEAFELAASGEITFSPCHEHQSVGSMAG
                     VTSASMWVHKVTNRTHGNTAYTNLSEQLSKILRFGANDQSVIDRLNWMRDVFGPVLAG
                     AMALNEDGLDLRLMLSQALHMGDEAHNRNVAGTTLLIQALAPYILETDFTTKEKREVF
                     DFVASSDYFSGPTWMVAAKASMDAANGIENSTIVTTMARNGVDFGIRVAGLGGQWFTG
                     PAQEVIGPMFAGYTREDSGLDMGDSAITETFGIGGFAMAAAPAIVALVGGTVDEAMGY
                     SRTMNAITTGNNPNITIPALDFMGVPSGIDVRKVMETGILPIINTAIAHKEPGIGMIG
                     AGITHPPVEAFQKALRALAETVAL"
     gene            complement(5483..7033)
                     /gene="fdrA"
                     /locus_tag="VN041_10585"
     CDS             complement(5483..7033)
                     /gene="fdrA"
                     /locus_tag="VN041_10585"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HWU29519.1"
                     /translation="MTRHIEIRKNTYLDSVSLMSMSTKANAVEGVTQALLGMATPMNK
                     EVLANVGVNDSAVDEARPSDLMIVIDANDDAVEAAIAAVNDILARKGKKAGGAHEVRY
                     RTLDGAFAENQDTNLVLISVNGAFAAREARKALNAGKSVMIFSDNVSVEDELSLKNLA
                     HEKGLIVMGPDCGTAIINGTGLAFANAVRRGSIGVVGASGTGSQEVSVRVHDFGGGIS
                     QLIGTGGRDLSTDIGGISMIDGIRALDADPETKVIVLISKPPAEEVAEKVLAVAGAAS
                     KPVFVTFLGSDRTESGHDNVTMVTAGTKPLAIAAVVASGIDESTLDKHPLNIPLIEEV
                     RAKLAPEQKYIRGLFCGGTLCDESMFAALAKYENVYSNIQKNPAYRIGAMDASKEHTF
                     LDMGDDDFTNGRPHPMIDPSLRLQRIVQEADDPEVGVIAMDFVLGFGSHEDPVGVTIP
                     AISEAKAKAAARGQHLEILGYVLGTDLDTPALAEQVAKLEAAGVTIASSSTNLGLLAR
                     EFVAKGDN"
     gene            complement(7055..7660)
                     /locus_tag="VN041_10590"
     CDS             complement(7055..7660)
                     /locus_tag="VN041_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWU29520.1"
                     /translation="MTDNSADQRYLDAAAAGDLDGVRAALAEGADRNAADWEDATAIL
                     HAARGRHLDVVRALIAEGVDIDAQDQTCSNPFLLGCITGDVELVRIMVEAGADLTRLT
                     RMGGNGLTPAAEKGHLEVVRYLLENTRINVNLTNNLGWTALIETIILGDGGPVRQEIV
                     QLLLAHRAKPGMTNEWGVSPADLARERGFTEIAAILDQAQA"
     gene            complement(7657..8976)
                     /locus_tag="VN041_10595"
     CDS             complement(7657..8976)
                     /locus_tag="VN041_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWU29521.1"
                     /translation="MTDSAAGTAAATASDASASDEFEREPVPESHRKSLFTVSAVWFG
                     FPMILTNAVPGGLVVAMLGFWQGVCAILVANAIMFVYVSLLSWRAGRTGKSFSLQAIE
                     TFGTAGYAIASGFLSTVVVGWFAFNTGATGATLSEAFGWNEMLVAAIAGVVFIAVTYL
                     GIRALSVLGTIAAPLFIVAVIVAVVIASQTADFSAVLSYGGEKVNDAMTFGVAVTAIM
                     ATFADSGTMTADFTRWARNGKEAVLATVTAFPIASLVAQLSGGVLVAAGAIAMPAVNG
                     GNFAPILTGTGNPLLNVFLLLFVVINLGSVCTHCLYNGALGWSHLTRSKMRLLTLILG
                     VIGVIVAVLGAWQFFANWLALLGVLVPPLGAVLIADQIILARRNAGRPEKRFRVSALV
                     AWAIGAGFALTSHFFAPALSDAVVGLVVGCLAYLALEQFTPKKEITR"
     gene            complement(8969..9943)
                     /locus_tag="VN041_10600"
     CDS             complement(8969..9943)
                     /locus_tag="VN041_10600"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HWU29522.1"
                     /translation="MTPVAIPVALTAASWDAALEETLSCAAERAFADGTRATALSART
                     PAHVHSVHRRVVNVLIDDVLVALASLDVDDAPATVRVPVEDWVRLGVTQDSPIAVSAD
                     RIVLAGDGHDVVVSLTGARAWSTSASDLARLGVEELTAAVAVLDGFRASEPVTPFGHA
                     AQELLVTRTARLRRELLDGDARSVHAAASSLIGLGEGLTPSGDDILTGLVFLAAQPGA
                     LLSRRLPSLVAAIRDGRTSTGLLSHVTLSHAAEGRARQSLHDLAGSLVSGDLDRMRSV
                     ASAILEIGHTSGADILTGIRLALDVERSLRASRSAAATPPTESRIPLD"
     gene            10086..11042
                     /locus_tag="VN041_10605"
     CDS             10086..11042
                     /locus_tag="VN041_10605"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HWU29523.1"
                     /translation="MRVVVALGGNALARRGEPMTAEFLRSNVKSTCESLANLARDNEV
                     VITHGNGPQVGLLALQNLAYQDVAAYPLDILGAETQGMIGYVVQQELSNALNGEREVA
                     GIITTTVVDENDPAFSRPTKLIGPTYSAHDAAEAAAEYRWTIAKDGSAFRRVVPSPNP
                     ISIVQAPLIRRLLEAGSLAVCVGGGGVPVREDSKGRHIGVQAVVDKDLASAALAADIG
                     ADTLILLTDGDYVSENWGTPEQRDIHTASVDAVAAMKFAEGSMQPKIDAAIRVGRAGG
                     RTLIGPLDRLDDLLARRIGTEIVPALDEGIRWVEKAPAGKKA"
     gene            11078..11752
                     /locus_tag="VN041_10610"
     CDS             11078..11752
                     /locus_tag="VN041_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29524.1"
                     /translation="MSRAEERELESTRVTAWLRDAIIIGERAPGSKLIERDLAAEIGV
                     SRVPVRDALKVLEAEGLVELRPRTWAIVREFTAADLADLDEVRQVLEPMAFRLAAERH
                     RRDGLERLRAALEDEQESARSADQLISRRAAADFHEIVVDLADNRLLTDLMGGIRSRL
                     RWALAQHDDLHHIAEEHVALYEAIRDRDGARAADLAYRHVESSRLERIAHTEALRTSS
                     MPVIAD"
     gene            11841..13064
                     /locus_tag="VN041_10615"
     CDS             11841..13064
                     /locus_tag="VN041_10615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWU29525.1"
                     /translation="MTRRTLDQSSKLKNVLYEIRGKALIEAARLEAEGHQILKLNTGN
                     PAVFGFEAPHQIVRDMLAALPTAHGYSESKGIISARRAVVSRYEEIPDFPRFDPDDVF
                     LGNGVSELITMVMQALLDQGDEVLIPAPDYPLWTAMTSLAGGTPVHYVCDEENGWQPD
                     LEDIRAKVTPQTKAIVIINPNNPTGAVYSREVLEGIVQIARENQLLLLSDEIYDRILF
                     DDAVHTPTATLAPDLLCLTFNGLSKTYRVAGYRSGWLVITGPKSHARGFLEGITLLAS
                     TRLCPNVPAQHAVQAALGGVQSIDALIVPSGRLHEQRDIAWKGLESIPGVSCVKPEGA
                     LYAFPRLDPEVHEIRDDERLVYDLLVSEKILLVQGTGFNWPNPDHLRVVTLPEARVLS
                     EAVERLGNFLASYRQ"
     gene            complement(13012..13386)
                     /locus_tag="VN041_10620"
     CDS             complement(13012..13386)
                     /locus_tag="VN041_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29526.1"
                     /translation="MLSADPLGVSRFIRAHAELRHVLGGTPVTVVANRVRPGPLGIDA
                     RGQIRRTLDRFAGIDDVAFVPEDQRAADAAALHARAIADVSPRSPLVTAVRRIASSLG
                     ARAETATAGSSRGSSRVARRLR"
BASE COUNT         2286 a         4499 c         4748 g         1997 t
ORIGIN      
        1 gggcagacac cggccgtccg ccgcgagcag tacgccgcag acatcacgta cggcacgaac
       61 aacgagttcg gcttcgacta cctgcgtgac aacatggcct ggcgcaagga ggatctcgtc
      121 cagcgggagc acttcttcgc gatcgtcgac gaggtcgact cgatcctgat cgacgaggcg
      181 cgcacgccgc tcatcatctc ggggccgtcc tccggcgagg cgaatcgctg gttcaccgag
      241 ttcgcgaaga tcgcccgcac gctcgagccc ggcgaggact acgaagtcga cgagaagaag
      301 cgcacgatcg gtgtgctcga gcccggcatc gagaaggtcg aggactacct cggcatcgac
      361 aacctctacg agtcggcgaa caccccgctg atctccttcc tgaacaactc gatcaaggcg
      421 atctcgctgt tcaagcgcga caccgactac gtcgtcatga acgacgaggt gatgatcgtc
      481 gacgagcaca ccggccgcat cctggtcgga cgccgttaca acgagggcat ccaccaggcg
      541 atcgaggcca aggagaacgt cccggtcaag gccgagaacc agacgctcgc gaccgtcacg
      601 ctgcagaact acttccgcct gtacgacaag ctcgccggca tgaccggtac cgccgagacc
      661 gaggcggccg agttcatgtc gacgtacaag ctcggcgtgg tcccgatccc gacgaacaag
      721 ccgatgatcc gcaaggacca gccggacctc gtctacaaga acgagcaggc caagttcgcg
      781 caggtcgtcg aggacatcgc cgagcgccac gcagcggggc agccggtgct ggtcggaacc
      841 gtcagcgtcg agaagagcga gtacctctcc cggctgctgg cgaagagggg cgtcaagcac
      901 gaggtgctca acgccaagaa ccacgctcgc gaggccgaga tcgtcgccct cgccggaaag
      961 ctcggtgccg tcacggtcgc gaccaacatg gccggccgag gcaccgacat catgctcggc
     1021 ggcaacgcgg agttcctcgc cgtccaggag ctcaagtcca agggactgga ccccgaggag
     1081 acgccggagg agtacgaggc cgcgtgggac gagacctacg aggccatgaa gaagaaggtc
     1141 gccgaggact cggtcaaggt gaccgaggcc ggtgggctgt acgtgctcgg caccgagcgt
     1201 cacgaatcgc gccgaatcga caaccagctg cgcggtcgct cgggccgaca gggcgacccc
     1261 ggcgagagcc gcttctacct gagcctgacc gacgacctca tgcgcctgtt ccagtcgggg
     1321 gcggccgagg cgatcctcgc gcgcaccaac ttcccggacg acgtcccgat cgagtcgggt
     1381 ctggtcagcc gcgccatccg cagcgcccag tcgcaggtcg aggcccgcaa cgccgagatg
     1441 cgcaagaacg tgctcaagta cgacgacgtc ctgaaccgtc agcgcgaggc gatctacgcc
     1501 gaccgccggc agatcctgca cggcgatgac atcgccgacc gggtcaagca cttcatcgag
     1561 gacgccatcg gcggcgtggt cgacgaccac accggcgagg gccacaacga gagctgggac
     1621 ttcgacgccc tctggaccga gctgaagacg ctgtacccga tgaacgtcac gatcgacgag
     1681 gtcgtcgccg aagcgggcgg acgcaagggc ggcatctccg ccgaggggct caagcgcgag
     1741 ctgctctccg atgccctgat cgcgtaccag aagcgcgagg aggccctcgg tgaggcggcc
     1801 acccgcgagc tcgagcgccg cgtcgtgctg caggttctcg accgtcgctg gcgcgaccac
     1861 ctgtacgaga tggactacct gaaggacggc atcggcctgc gcgcgatggc gcagcgcgac
     1921 ccgctgatcg agtaccagcg cgagggctac gcgatgttcc agtcgatgat ggggcagatc
     1981 aaggaggagt cggtcggcta cctctacaac ctcgaggtcg aggtgcgccg cgccggcgcc
     2041 gaccagaccg aggtcgaggc gaagggtctg gtcgaccagc agcccgagca gaagctggag
     2101 tactcggcgc ccaacgatgc gggcgacgtg gaggtccgca acgagcgcgg ccaggtgcag
     2161 caggccgcca ccgagcgcgc cgccaccgcc cagcgcggtg cgttcggcca gcaggtggat
     2221 gccgaggagc agccggctcc ggcgaaccgc gagcagcgcc gcgcgcagag caagaagaag
     2281 aagtaagcgc ctgcgcgaac gaagaaggcc ggccccctgg gccggccttc ttcgttcgca
     2341 cggactgtgg tgctccgtgc aggatggagt tcgtgcgccg aggggtacgg gagcgcgaac
     2401 tcccgtaccc ctcacatgga tgactcagcc cttggaggac tggatcagcg acggcgcgac
     2461 ggcgttgctg agcgtgatct cgtcgttcgc ggtgaaggtc accgactcgt tcgtgatccg
     2521 gtcctggatc cacttcgcca ggaacagcgc cgccaccatc aggtaggcga cgaggaacat
     2581 cacgtgcgtg tcggacagcg cgaacttctc gggcaggccc gggacatgcg cgtggatgac
     2641 gccgaagaac gacaggacgc ccgccagtgc accgatgatc gcgccctgca ggggcttgtt
     2701 gcggatcgcg aagatcgcga tcatgcccca cagcagcgac gacagcggtg cgccgttgcc
     2761 gagcgtggag atgccggcga agtacgtgcc ggcgtggttc agagcatccg ctcccacttc
     2821 ggccgtgttc gtgcccgccg ccttcagcgt gttgttgatc aggctgttcg cccagttcgc
     2881 gatccacggg aacagcgcga tgaagatcac cggcagctcc accttcggcg tctcacgcac
     2941 cgactgcgcg gcggtgacga caccgatgta gatcaggatc ggcgcgatcg ccgccatcgg
     3001 gaccaccgac agcagcaggg cgctcatgcc gaacagtccc accgcgaaca tcgtgatgcc
     3061 gttcagcatc gtgtagccga tcccggcgcc catctccttg tacgcggtgt gcccgatgta
     3121 gacggtgacc gggtacgggt tgcccatcag ggcgccgacc atcgtggcgc cgccgttgac
     3181 gagcatcacg ggacgggcgc tgtagtggtc accggcggcc tcggccgcct cgatgttctc
     3241 caggtcgaac acgtagttcg ccagcgccag cggcaccgcc gaggcgagga acggtccggc
     3301 gtgggcgacc ccgtcgatga agttcgtgat cgacacctgc ggcgggtgcc accccgcgtg
     3361 caccagcgcg gcctgaaccg cctccggcgt ctgcaggccg aacgcccagg cggcggccgt
     3421 gcccacgatc agcagcagca gaccggtcgg aaccttgccc aggaacggcg agcggccgaa
     3481 ccagttcatc agcacgagcg tcagcacgac gaacgcgacg acggggaact ggaaggactg
     3541 cagcatcggg ttcatcgcca gcagcagcag acccagtccc gcaaggcagg ccagcagcac
     3601 cgagcggggg accgcccgac gcagcagatc gccgaagaac gagcccacga acaggatcag
     3661 accctcgagg aagccccaga cgaggccgat gcccacggcg agttcggcat cctgcgtggt
     3721 cgagtagacc ggcaggatca ccaggaacgt gaccgtgaag atcgagggcg cactgggacc
     3781 ggagggaagg gccacgacgt cgtcgcgacc ctccttggcg gcgaggcgct tggcgaacca
     3841 gacgtacatc gaggacgtca gcaggatcgc cagaccgaac gccggcgcga tgcgcccgac
     3901 caccatgtcc accgggagcc ccgccatggt gagcagtgcg gtgaaggtga tgatgttggt
     3961 caggttgttg gtgaacagcg cgaagaacgc cgcgatgtca cccttcttcc accatttcag
     4021 ctggccggcg gtggacacgg atgccggcgc gggggcggtc acagcgcgac cgtctcggcc
     4081 agggcgcgga gcgccttctg gaaggcctcg accggcgggt gggtgatgcc cgcgccgatc
     4141 atgccgatgc ccggctcctt gtgggcgatg gcggtgttga tgatcggcag gatgccggtc
     4201 tccatcacct tgcgcacgtc gatgccgctg gggacgccca tgaagtcgag cgcggggatc
     4261 gtgatgttcg ggttgttgcc cgtcgtgatc gcgttcatcg tgcgcgagta gcccatggcc
     4321 tcgtcgacgg tgccgccgac gagggcgacg atcgcgggag cagcggccat cgcgaaaccg
     4381 ccgatgccga aggtctcggt gatggcggag tctcccatgt ccagaccgga gtcctcacgg
     4441 gtgtagccgg cgaacatcgg gccgatgacc tcctgcgcgg ggccggtgaa ccactgcccg
     4501 ccgaggcctg cgacgcggat gccgaagtcg acgccgttgc gggccatggt ggtgacgatc
     4561 gtcgagttct cgatgccgtt cgccgcgtcc atcgatgcct tcgccgcgac catccaggtc
     4621 gggccggaga aatagtccga ggaggcgacg aagtcgaaca cctcgcgctt ctccttcgtg
     4681 gtgaagtcgg tctcgaggat gtacggtgcc agcgcctgga tcagtagggt ggtgccggcc
     4741 acgttgcggt tgtgcgcctc gtcgcccatg tgcagcgcct gcgagagcat cagacgcagg
     4801 tccaggccgt cctcgttcag cgccatggcg ccggcgagca cgggaccgaa cacgtcgcgc
     4861 atccagttca ggcggtcgat gaccgactgg tcgttggcgc cgaagcgcag gatcttcgac
     4921 agctgctccg acaggttggt gtacgcggtg ttgccgtgtg tgcggttggt gaccttgtgc
     4981 acccacatgc tggcgctggt gacaccggcc atcgagccga ccgactggtg ctcgtggcag
     5041 ggcgagaagg tgatctcgcc cgaggccgcg agctcgaacg cctcgtcgac gtccttcgcc
     5101 aggccctcga agacgagcgc gccggtgacg gcgcccttca tcgggcccga catccgggtg
     5161 aactcgatcg gcggaccggc gtgcaggatg gtcttcgcgg tcatcccggg cacggtgtcg
     5221 atcgcctgac cgaagccctc caggaagggc tgcgaggaga ggatgcgctc gagggcgacc
     5281 tcgttggcgg cgtcgatctt ctcggcgacc gcggggtcct cgagtcggct cagcgcggcg
     5341 atgacctccg ggtcgccacc gcccggcggg gtccagtcca tcaggaccgg ctcgacgccc
     5401 tgcgtcttca ggtcgtcggc gaacatgtcg aggccgaggt tgaccggctt cagctcggag
     5461 gagaaaaggt cgttgacgct catcagttgt cacccttcgc gacgaattca cgggccagca
     5521 ggccgaggtt ggtggaggag gaggcgatgg tcacgccggc ggcctcgagc ttcgcgacct
     5581 gctcggcaag cgccggggtg tccaggtcgg tgccgaggac gtagccgagg atctcgaggt
     5641 gctgaccgcg ggcggccgcc ttcgccttgg cctcggagat cgccgggatg gtgacgccga
     5701 cggggtcctc gtgcgagccg aagccgagca cgaagtccat ggcgatgacg ccgacctcgg
     5761 ggtcgtccgc ctcctggacg atgcgctgca gacgcagcga cgggtcgatc atcgggtgcg
     5821 gacgcccgtt ggtgaagtcg tcgtcgccca tgtcgaggaa ggtgtgctcc ttcgaggcat
     5881 ccatggcgcc gatccggtac gccgggttct tctggatgtt cgagtacacg ttctcgtact
     5941 tggcgagcgc cgcgaacatc gactcgtcgc acagcgtgcc accgcagaac aggccgcgga
     6001 tgtacttctg ctcgggggcg agcttggcgc gcacctcctc gatgagcggg atgttcagcg
     6061 ggtgcttgtc cagcgtcgac tcgtcgatgc cggaagccac gacagcggcg atcgcgaggg
     6121 gcttggtgcc cgccgtgacc atcgtgacgt tgtcgtggcc ggactcggtg cggtcagagc
     6181 ccaggaaggt cacgaagacc ggcttagagg cggcgccggc gacggccagg accttctccg
     6241 cgacctcctc cgcaggcggc ttggagatga ggacgatgac cttggtctcg gggtcggcgt
     6301 ccagggcccg gatgccgtcg atcatcgaga tgccgccgat gtcggtgctg aggtcgcgac
     6361 cgccggtgcc gatcagctgc gagatgccac cgccgaagtc gtgcacgcgc accgacacct
     6421 cctggctgcc ggtgcccgag gcgccgacga cgccgatgct gccgcggcgc accgcgttgg
     6481 cgaacgccag gccggtgccg ttgatgatcg cggtgccgca gtcggggccc atcacgatca
     6541 ggcccttctc gtgggccagg ttcttcagcg acagctcgtc ctcgacggac acgttgtcgg
     6601 agaagatcat gacgctcttg ccggcgttca gcgccttgcg cgcctcccgc gccgcgaacg
     6661 cgccgttgac cgagatcagg acgaggttcg tgtcctggtt ctccgcaaag gcgccgtcca
     6721 gcgtgcggta gcgcacctcg tgcgccccgc cggccttctt gcccttgcgg gccaggatgt
     6781 cgttgaccgc cgcgatggcg gcctcgacgg cgtcgtcgtt cgcgtcgatc acgatcatca
     6841 gatcgctcgg cctggcctcg tcgacggcgg agtcgttgac gccgacgttg gcgagcacct
     6901 ccttgttcat cggggtcgcc atgcccagca gggcctgggt gacgccctcg acggcgttcg
     6961 ccttggtgct catcgacatg agcgagacgg agtcgaggta ggtgttcttc ctgatttcga
     7021 tgtgcctggt catcgttgtc ctttcggggg agtcttaggc ctgcgcctgg tcgaggatgg
     7081 cggcgatctc ggtgaacccg cgctcgcgcg cgaggtccgc cggggacacg ccccactcgt
     7141 tggtcatgcc gggcttggcg cggtgcgcga gcagcagctg gacgatctcc tggcggacgg
     7201 ggccgccgtc gccgaggatg atggtctcga tcagggcggt ccagccgagg ttgttcgtga
     7261 ggttcacgtt gatgcgggtg ttctccagca ggtagcggac gacctcgagg tggcccttct
     7321 cggccgccgg cgtcaggccg ttgccgccca tgcgcgtcag gcgcgtgagg tcggcgcccg
     7381 cctcgaccat gatcctcacg agctcgacgt caccggtgat gcagccgagc aggaacgggt
     7441 tggagcaggt ctggtcctgg gcgtcgatgt cgacgccttc cgcgatgagc gcgcgcacca
     7501 cgtcgaggtg ccggccgcgg gcggcgtgca ggatcgcggt ggcgtcctcc cagtccgcgg
     7561 cgttcctgtc cgcaccctcg gcgagggcgg cgcgcacgcc gtccagatcg cctgctgcgg
     7621 cggcatccag atagcgctgg tccgcgctgt tgtctgtcat cgggtgatct ccttcttggg
     7681 ggtgaactgc tccagggcca ggtacgcgag gcatcccacg accaggccga ccacggcatc
     7741 cgagagggcc ggggcgaaga aatgcgaagt cagggcgaag ccggcgccga tcgcccaggc
     7801 gaccagagcg ctgacgcgga accgcttctc ggggcggccg gcgttgcggc gggcgaggat
     7861 gatctggtcg gcgatcagca cggcgcccag cggagggacg agtacgccga gcagtgcgag
     7921 ccagttggcg aagaactgcc acgcgccgag cacggcgacg atcacgccga tgacgccgag
     7981 gatcagcgtc agcaggcgca tcttcgaccg ggtcaggtgg gaccagccga gggcgccgtt
     8041 gtacaggcag tgcgtgcaga cggagccgag gttgatcacc acgaacagca gcaggaacac
     8101 gttcagcagc gggtttccgg tgccggtgag gatcggtgcg aagttgccgc cgttcaccgc
     8161 gggcattgcg atggcccctg cggcgacgag cacgccgccg ctgagctgtg cgacgaggct
     8221 ggcgatcggg aaggcggtca cggtggccag cacggcctcc ttgccgttgc gcgcccagcg
     8281 ggtgaagtcg gcggtcatcg tgccggagtc cgcgaaggtc gccatgatcg cggtgacggc
     8341 cacgccgaag gtcatcgcgt cgttcacctt ctctccgccg tagctcagca cggcggagaa
     8401 atccgcggtc tgcgacgcga tgaccaccgc gacgatgacg gcgacgatga acagcggtgc
     8461 ggcgatggtg ccgagcaccg agagcgcgcg gatgccgagg taggtgacgg cgatgaacac
     8521 gacgccggcg atggcggcca cgagcatctc gttccagccg aacgcctcgc tgagggtcgc
     8581 gccggtcgca cccgtgttga acgcgaacca gcccacgacg accgtggaga ggaagccgga
     8641 ggcgatcgcg tagccggcgg tgccgaaggt ctcgatcgcc tgcagcgaga agctcttccc
     8701 ggtgcggccg gcgcgccagc tgagcaggct gacgtagacg aacatgatcg cgttggcgac
     8761 gaggatcgca cagacaccct gccagaagcc cagcatggcg accaccaggc cgccgggaac
     8821 ggcattggtc aggatcatcg ggaacccgaa ccagacggcc gagaccgtga acagtgactt
     8881 gcggtggctc tccgggaccg gctcgcgctc gaactcgtcg gatgccgagg catccgatgc
     8941 ggtcgccgcg gcggtgcctg cagctgaatc agtcaagagg gatcctcgat tccgtcggag
     9001 gggtggcggc tgcgctccgg gaggcgcgga gcgacctctc gacgtcgagc gcgagccgga
     9061 tgccggtgag gatgtccgca ccggacgtgt ggccgatctc gaggatcgcg gaggcgacgg
     9121 agcgcatgcg atcgagatca ccggagacga gggagccggc cagatcgtgc aggctctggc
     9181 gcgcacggcc ctcggctgcg tgcgacaggg tgacgtggct gagcaggccc gtcgaggtcc
     9241 ggccgtcgcg gatggcggcg acgagggacg gcaggcggcg gctcagcagt gcgccgggct
     9301 gggcggcgag gaagacgagg ccggtgagga tgtcgtctcc ggaaggggtg agtccctcgc
     9361 ccagcccgat cagcgagctc gcggcggcgt gcacggaccg agcgtcgccg tcgaggagct
     9421 cgcggcgcag ccgcgccgtc cgggtcacca ggagctcctg tgccgcatgt ccgaacggcg
     9481 tgacgggttc ggatgcccgg aatccgtcga gcacggccac ggccgcggtg agctcctcga
     9541 cgccgagccg cgccagatcg gatgccgagg tgctccacgc gcgtgcgccg gtgagggaca
     9601 cgacgacgtc gtgaccgtcg cccgcgagca cgatgcggtc cgcggagacg gcgatggggg
     9661 agtcctgcgt cacacccagc cgtacccagt cctcgacggg gacgcggacc gtggcgggcg
     9721 catcatcgac gtcgaggctc gcgagcgcga ccaggacgtc gtcgatgagg acgttgacca
     9781 cccggcgatg caccgagtgc acgtgagccg gggtgcgggc ggaaagcgca gtggcgcgcg
     9841 tgccatcggc gaacgcgcgc tctgctgcgc atgagagcgt ctcctcaagc gcagcatccc
     9901 agctggccgc cgtcaaggcg accgggattg cgacgggggt catcgtcttc tctccatgac
     9961 ggtctcaccg tggatgtgcg atgcgacccg tcttcacttt gtgtcccctc aggtatacca
    10021 ttcaagcgga tggaatacca ttccaaaatt tggaatacca aaagtcttct actgaaggag
    10081 taagcatgcg cgtcgtagtg gcactcgggg gcaacgccct cgcccgtcgt ggtgagccga
    10141 tgaccgccga gttcctgcgc tcgaacgtga agtcgacgtg cgaatcgctg gcgaacctgg
    10201 cccgtgacaa cgaggtcgtc atcacgcacg gcaacggccc gcaggtgggt ctgctcgcgc
    10261 tgcagaacct ggcataccag gacgtcgccg cgtacccgct ggacatcctc ggcgccgaga
    10321 cccagggcat gatcggctat gtcgtccagc aggagctctc gaacgcgctg aacggcgagc
    10381 gcgaggtcgc cggcatcatc acgaccacgg tcgtcgatga gaacgacccg gccttctcgc
    10441 gtcccaccaa gctcatcggc ccgacctact ccgcccacga cgccgccgag gccgcggccg
    10501 agtaccgctg gaccatcgcc aaggacggca gcgccttccg ccgcgtcgtg ccctcgccga
    10561 acccgatctc gatcgtgcag gccccgctga tccgccgcct gctcgaggcg ggcagcctcg
    10621 ccgtgtgcgt cggcggtggc ggggttccgg tgcgcgaaga ctcgaaggga cgccacatcg
    10681 gcgtccaggc ggtggtcgac aaggacctcg cctcggccgc cctggccgcc gacatcggtg
    10741 ccgacaccct catcctgctc accgacggcg actacgtcag cgagaactgg ggcacccccg
    10801 agcagcgcga catccacacc gcttccgtcg acgcggtcgc ggcgatgaag ttcgccgagg
    10861 gctccatgca gccgaagatc gacgccgcga tccgcgtcgg ccgcgccggt ggccgcacgc
    10921 tgatcggtcc gctggaccgc ctcgacgacc tcctggcccg ccggatcggc accgagatcg
    10981 tgccggcgct cgacgagggc atccgctggg tcgagaaggc gcccgcagga aagaaggcct
    11041 gaccggcttc tcgccactgg cgtgcgagga tgaatgcgtg agcagagcag aagagcgtga
    11101 gctcgagtcg acgagggtca ccgcgtggtt gcgcgacgcg atcatcatcg gcgagcgtgc
    11161 tccgggcagc aagctgatcg agcgcgacct cgcggccgag atcggcgtca gccgcgtgcc
    11221 cgtccgcgac gcgctcaagg tcctcgaggc cgagggcctg gtcgaactgc gaccgcgcac
    11281 ctgggcgatc gtccgtgaat tcaccgccgc agacctggcg gacctcgacg aggtccgcca
    11341 ggtgctcgag ccgatggcgt tccgcctcgc tgcggagcgc catcgccgag acggcctgga
    11401 gaggttgcgc gccgccctgg aggacgagca ggagagtgcc cgcagcgccg accagctcat
    11461 ctcccgacgt gcggcggccg acttccacga gatcgtcgtg gatctggccg acaaccgcct
    11521 gctcaccgac ctgatgggcg gcatccgcag ccggctgcgc tgggcgctcg cacagcacga
    11581 cgacctgcat cacatcgccg aggagcacgt cgcgctctac gaggcgattc gggaccgcga
    11641 cggcgcccgc gccgcggatc tcgcctaccg gcacgtcgag agcagccggc tcgagcgcat
    11701 cgcgcacacc gaggcgctgc gcacctcctc gatgcccgtc atcgccgact gaacgacgac
    11761 cgctccgtcg tcctccggga cgaccccggc ggcgtggaac ggtggcgggc cggcccctgc
    11821 gctgtcggta tccttggcag atgacccgcc gaaccctgga ccagtcgtcc aagctcaaga
    11881 acgtcctcta tgagatccgt ggcaaggcac tgatcgaggc ggcgcgtctg gaggcggagg
    11941 ggcatcagat cctcaagctg aacacgggca acccggcggt cttcggcttc gaggcgccgc
    12001 accagatcgt gcgcgacatg ctcgccgcgc tcccgaccgc gcacggctac agcgagagca
    12061 agggcatcat ctccgcccgc cgtgcggtgg tcagccgcta cgaggagatc cccgacttcc
    12121 cccgcttcga cccggacgac gtgttcctgg gcaacggcgt ctccgagctc atcaccatgg
    12181 tcatgcaggc gctgctcgat cagggcgacg aggtcctgat ccccgcgccg gactacccgc
    12241 tgtggaccgc catgacctcg ctggccggtg gcactccggt gcactacgtc tgcgacgagg
    12301 agaacgggtg gcagcccgac ctcgaggaca tccgcgcgaa ggtgacgccg cagaccaagg
    12361 cgatcgtcat catcaacccg aacaacccca ccggcgcggt gtactcgcgc gaggtgctcg
    12421 aaggcatcgt gcagatcgct cgtgagaacc agctgctgct gctctcggac gagatctacg
    12481 accggatcct cttcgacgac gccgtgcaca cgccgacggc gacgctcgct cccgacctgc
    12541 tctgcctgac cttcaacggg ctgtcgaaga cctatcgcgt cgccggctac cgttcgggct
    12601 ggctggtgat caccggcccg aagtcccatg ctcggggctt cctggagggc atcactctgc
    12661 tggcctcgac gcgactgtgc ccgaacgttc ccgcgcagca tgccgtgcag gcggcgctgg
    12721 gcggcgtgca gtcgatcgac gcgctgatcg tcccgtccgg gcgcctgcac gagcagcgcg
    12781 acatcgcctg gaagggactg gaatcgatcc cgggcgtgag ctgcgtcaag cccgagggcg
    12841 ccctctacgc cttcccgcgg ctcgaccccg aggtgcatga gatccgcgat gacgagaggc
    12901 tcgtctacga cctgctcgtc tccgagaaga tcctgctggt gcaggggacc ggcttcaact
    12961 ggccgaaccc cgaccacctg cgcgtcgtca ccctccctga ggcgagggtg ctcagcgaag
    13021 ccgtcgagcg actcgggaac ttcctcgcga gctaccggca gtagccgtct cggcgcgtgc
    13081 gcccagcgat gaggcgatgc gacgcacggc ggtgaccagc ggcgaacgcg gcgacacgtc
    13141 ggcgatcgca cgcgcgtgca gagccgcggc atccgctgcg cgctgatcct cggggacgaa
    13201 cgcgacgtcg tcgatccccg cgaagcggtc cagcgtgcgc cggatctgtc cgcgtgcgtc
    13261 gatgcccagc gggccggggc gcacgcggtt cgcgaccacc gtcaccggtg tgccgcccag
    13321 gacatgcctc agctcggcgt gggcacggat gaaccggctc acaccgaggg ggtcggcaga
    13381 gagcaccgcc accacggcat cggcttccag cagggcggcg gcggtcgcgg cgggttgagc
    13441 gcaccggcgg tgggctgcgg ttccagcgcc agggtgtggc tgtcggtgcg gcggaaggtc
    13501 atgcccgaac cgctgagcag gcgttgcagt
//
LOCUS       DATMSX010000341         5516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_146979,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000341 DATMSX010000000
VERSION     DATMSX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146979"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VN041_10625"
     CDS             <1..840
                     /locus_tag="VN041_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HWU29527.1"
                     /translation="LPQLAIHLDRSANEGLALDKQTGTQPVWGLGDAASADILGELAA
                     AAGVDASEIRGYDAVVADAARGAVFGKDDAFFASGRLDDLASVHAGVVALIEASESPT
                     SGVIPVLAAFDHEELGSASRSGAAGPFLEDVLVRLYASFGADAIHQRRALAASWHLSS
                     DVGHSVHPNFAHKHDPVVQPLLGSGPILKINANQRYATDAVGAAAWAGWCESAGVGYQ
                     EFVSNNAVPCGSTIGPITATRLGIRTVDIGIPILSMHSARELAGVSDLHDLSRVARAF
                     FAG"
     gene            complement(876..1550)
                     /locus_tag="VN041_10630"
     CDS             complement(876..1550)
                     /locus_tag="VN041_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29528.1"
                     /translation="MNDEQILALEARARRHAALADPARLRMVDLLSAGDLSPTELRTR
                     IGMTSNLLAHHLNVLEREAIVSRGRSEGDGRRSYVRLDPSALSDLVPLSIIDAPRVVF
                     ICTANSARSQLARAIWNRNSPVPAVSAGTHPAPRIAPGAVEVAARHGFDLSDAVPTSA
                     DGVVGASDLIITVCDRAHEEYPTTALHWSVPDPVRGGTSEAFDAAFAELQSRVERLAP
                     RVRLVA"
     gene            1599..2300
                     /locus_tag="VN041_10635"
     CDS             1599..2300
                     /locus_tag="VN041_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWU29529.1"
                     /translation="MNHTSLPRRAAAELLGTGLLVAVVVGSGIAAERLSSDHGLRLLE
                     NSIATALGLAMLIVLLGPVSGAHFNPVITIADSLLRRDGAAAKDRAGAVTYIAAQLVG
                     GIGGALLANAMFETPAAVALHERATAATLLSEVVATAGLTLLVVGMTRAAHSLPAIAC
                     AVGAYIGAAYWFTSSTAFANPAVTLGRIFSDTFAGIAPLSAAFFLAAQLIGGAVGILL
                     AVILFPAPSLKEKTS"
     gene            2297..2698
                     /locus_tag="VN041_10640"
     CDS             2297..2698
                     /locus_tag="VN041_10640"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWU29530.1"
                     /translation="MTDHPTVLFVCVHNAGRSQMAAGFLQAIAGDRIDVRSAGSEPKD
                     AINPMAVQAMSEAGIDITTGAPKLLTIDAVKQADVVVTMGCGDACPIFPGKRYEDWQL
                     DDPAGQSIEAVRVIRDEIRSRVETLVAELAP"
     gene            2901..3248
                     /locus_tag="VN041_10645"
     CDS             2901..3248
                     /locus_tag="VN041_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU29531.1"
                     /translation="MPVQELIPLRDLQSCCAPLTRAPLSVENAASLASSLRAIADPAR
                     LRILSMIAAHADGEACVCDLTEPLGLSQPTVSHHLRVLVDAGFLEREKRGTWAFFRVA
                     DGALESLTGILGR"
     gene            3323..4780
                     /locus_tag="VN041_10650"
     CDS             3323..4780
                     /locus_tag="VN041_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWU29532.1"
                     /translation="MSASIEAASPALIERTGIEIIPESDRTAKPRDLFWPWFAANVSV
                     FGMSYGSFVLGFGISLWQATVVSVVGIIVSFFLCGLIAIAGKRGSAPTMILSRAAFGV
                     QGQKLPGIVSWLTSIGWETSLAITAVLATTTVFRSLGWLSADDQGVGLKIVATVAVAA
                     LIVAASVLGYHTIMKLQSVLTWLTGAMTVLFLILTFESINWSTAFSHPDGTLAQTIGA
                     LVMVMTGFGLGWINIAADWSRYQKRTASDGQIIAWNTIGGSVAPIILVFFGLLLAGSN
                     QKLMDAVGSDPIGALATLLPPWVLVPFLIVAILTLVSGAVLGIYSSGLTLLSLGIRIP
                     RPAAAGIDGVILTAGTIFVVFFATSFIGPFQSFLITLGVPMASWAGILIADILRRRRD
                     YDEAALFDSNGRYGAFDWTSIITMLVATGIGWGFVMNQIPDTPWNDWQGYFLDLFGWR
                     DDWGYANLGVLFALVLSFAATWFARAGRIRRQEQG"
     gene            4777..5370
                     /locus_tag="VN041_10655"
     CDS             4777..5370
                     /locus_tag="VN041_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWU29533.1"
                     /translation="MTEAPMSLGWVAEPVEASPHWLVVIDPQNIFASPDSEWGSPFFA
                     DAMVNIRSLADAHGERVLVTRWVPTADRATSWGDYFEEWPFADKPASDPLYDLVPDAA
                     ELSPHPTLDLPTFGKWGAELEAIVGRGAPVVLAGVSTDCCVLSTALAGADAGARLTVA
                     TDACAGSTAENHAAALHVLGLYPPQIALRTTAEILGG"
BASE COUNT          773 a         1993 c         1843 g          907 t
ORIGIN      
        1 ctgcctcagc tggcgatcca cctcgaccgc agcgcgaacg agggcctcgc cctcgacaag
       61 cagaccggca cgcagccggt gtgggggctc ggcgatgccg catccgccga catcctcgga
      121 gagctggccg ccgccgccgg cgtcgacgcc tcggagatcc gcgggtacga cgcggtcgtc
      181 gcggatgccg cgcgcggcgc cgtcttcggc aaggacgacg cgttcttcgc atccggccgc
      241 ctggacgacc tcgcgtcggt gcacgccggc gtcgtcgcgc tgatcgaggc gtcggagtcg
      301 cctaccagcg gagtgatccc ggtgctcgcc gcgttcgacc acgaggagct gggctcggca
      361 tcgcgctccg gcgccgccgg ccccttcctg gaggacgtgc tcgtccggct gtacgcctcg
      421 ttcggagcgg atgccatcca ccagcgccgt gcgctggcgg cgtcgtggca cctgtccagc
      481 gacgtcggcc actcggtgca cccgaacttc gcccacaagc acgaccctgt ggtgcagccg
      541 ctgctgggct ccggccccat cctgaagatc aacgccaacc agcgctacgc gacggacgcc
      601 gtgggtgccg cggcctgggc gggctggtgc gagagcgccg gggtcggcta tcaggagttc
      661 gtgtcgaaca acgccgtgcc gtgcggctcg accatcggcc cgatcaccgc gacgcgcctg
      721 ggcatccgca cggtcgacat cggcatcccg atcctgtcga tgcattccgc gcgcgagctc
      781 gccggcgtca gcgacctcca cgacctctcc cgcgtggcgc gggcgttctt cgcgggctga
      841 ggtccaggcg ccgaggtccg ctcatcgcgc cagggtcaag ccaccaggcg gacccgtggc
      901 gcgagccgtt cgacccggct ctgcagttcg gcgaaagccg cgtcgaaggc ctcggacgtg
      961 cctccccgca ccgggtcggg aaccgaccag tgcagcgccg tcgtggggta ctcctcgtgg
     1021 gcgcggtcgc acacggtgat gatcaggtcg gatgcaccga cgacgccgtc cgcgctcgtc
     1081 ggcaccgcgt cgctgaggtc gaatccgtga cgagcagcca cctcgacggc tcccggggcg
     1141 atgcgcggag ccggatgcgt gcccgcgctc actgcgggca ccgggctgtt cctgttccag
     1201 atcgctcggg ccagttgaga gcgagcggag ttcgccgtgc agatgaacac cacgcggggc
     1261 gcgtcgatga tgctgagcgg gacgagatcg gacagcgccg acgggtcgag tcgcacgtac
     1321 gaacgacgcc catcgccctc ggagcgccct cgggagacga tcgcctcgcg ctcgagcacg
     1381 ttgaggtgat gcgcgagcag gttcgacgtc atgccgatgc gcgtgcggag ctcggtcggc
     1441 gagaggtcgc ctgccgagag caggtcgacc atgcgcagcc gggcgggatc ggcgagcgcg
     1501 gcgtgacggc gcgcccgcgc ctcgagggcg agaatctgct catcattcat tgcctcaatc
     1561 ttgactgagc taactgaacc cgtcaagatg atctcgtcat gaaccacaca tctctgcccc
     1621 gtcgcgcggc agccgagctc ctgggcaccg gtctgctcgt cgccgtcgtc gtcggctcag
     1681 gcatcgccgc ggaacgactg agcagcgacc acggattgcg cctgctggag aactcgatcg
     1741 cgacggcgct gggcctggcg atgctgatcg tgctgctcgg cccggtttcg ggcgcacact
     1801 tcaacccggt gatcaccatc gcggactcgc tgctgcgacg cgacggcgca gcagcgaaag
     1861 accgcgcagg agcggtgacc tacatcgccg cccagcttgt cggcgggatc ggcggcgcgc
     1921 tgctcgcgaa cgccatgttc gagacgccgg ccgcggtcgc gctgcacgag cgcgcaacgg
     1981 ccgcaaccct cctcagcgag gtcgtcgcca ccgcaggact gacgctgctc gtcgtcggaa
     2041 tgacacgcgc cgcgcacagc ctgcccgcca tcgcctgcgc tgtcggcgcc tacatcggcg
     2101 ctgcatactg gttcaccagc tcgacggcgt tcgcgaatcc ggccgtcacc ctcggccgca
     2161 tcttctccga caccttcgcc gggatcgccc cgctctcggc cgcgttcttc ctcgcggcgc
     2221 agctgatcgg cggcgcggtc gggatcctgc tcgccgtcat cctgttcccc gcaccttccc
     2281 tcaaggagaa gacatcgtga ccgaccaccc caccgtgctc ttcgtctgcg tacacaacgc
     2341 cgggcgctcg cagatggccg cgggcttcct gcaggcgatc gccggcgacc gcatcgatgt
     2401 gcgctcggcc ggctccgagc cgaaggacgc gatcaacccc atggccgtgc aggccatgtc
     2461 ggaggccggg atcgacatca cgaccggcgc cccgaagctg ctgacgatcg atgccgtgaa
     2521 gcaggccgac gttgtcgtca cgatgggctg cggcgacgca tgcccgatct tccccggcaa
     2581 gcgctacgag gactggcagc tcgacgatcc tgccggccag agcatcgagg cggtgcgcgt
     2641 gatccgcgac gagatccgct cgcgcgtcga gacgctggtc gccgaactgg ctccctgaac
     2701 ccgccgcacc gctcgcggcg ggttcagggg cttcatctca gcagcagccg gtggggcagg
     2761 agccgtcggg cagcggctcg cagccggtgt cgcagcacgc gtccatgtca cacctccctt
     2821 catcgatgag catcgatatt tgacgttgcg atcgtcgcgc atatattgac gactgtcaat
     2881 atatggagca gaatcgacac atgccggtcc aagagctgat ccccctgcgc gatctgcagt
     2941 cctgctgcgc cccgctcacc cgcgcgccgc tgtccgtcga gaacgccgcc tctctggcat
     3001 cctcgctcag ggccatcgcc gaccccgccc gtctccgcat cctgtcgatg atcgccgcac
     3061 acgccgacgg cgaggcgtgc gtctgcgacc tcaccgagcc gctcggcctc agccagccca
     3121 ccgtctccca tcacctgcgc gtcctcgtcg acgccgggtt cctcgagcgc gagaagcgcg
     3181 gcacctgggc gttcttccgc gtcgccgacg gcgcgctgga gtccctcacc ggcatcctcg
     3241 gtcgctgagg cagcgcgtcc cctcgcgcgg tggcggcgcc ggatgccagg atgagcacaa
     3301 cccgaacatc ccggaggcac ccatgtccgc gagcatcgaa gccgcaagcc ccgctctgat
     3361 cgagcgcacc ggcatcgaga tcatccccga gtccgaccgc acggccaagc cgcgcgacct
     3421 gttctggccg tggttcgcgg cgaacgtgtc ggtgttcggc atgtcgtacg gatcgttcgt
     3481 gctcggcttc ggcatctcgc tgtggcaggc gacggtggtc tcggtcgtcg ggatcatcgt
     3541 gtcgttcttc ctgtgcgggc tgatcgcgat cgccggcaag cggggctcgg cgccgacgat
     3601 gatcctgtcc cgcgccgcct tcggcgtgca gggccagaag ctgcccggca tcgtgtcgtg
     3661 gctcacgtcg atcggctggg agacctcgct ggcgatcacg gccgttctcg ccaccacgac
     3721 ggtgttccgc agcctgggct ggctgagcgc cgacgaccag ggcgtcgggc tgaagatcgt
     3781 ggcgaccgtc gcggtggcgg cgctcatcgt cgcggcatcc gtgctcggtt accacacgat
     3841 catgaagctg cagtcggtgc tgacctggct gaccggcgcg atgaccgtgc tgttcctgat
     3901 cctcacgttc gagagcatca actggtcgac cgcgttctcg cacccggacg gcactctcgc
     3961 gcagaccatc ggcgcgctcg tgatggtgat gaccgggttc ggcctgggct ggatcaacat
     4021 cgccgccgac tggtcgcggt accagaagcg caccgcatcc gacgggcaga tcatcgcctg
     4081 gaacacgatc ggcggctcgg tggcgccgat catcctggtc ttcttcggac tcctgctggc
     4141 aggctcgaac cagaagctga tggatgccgt gggatccgac ccgatcggag ccctcgccac
     4201 cctgctgccc ccgtgggtgc tggtgccgtt cctgatcgtc gcgatcctca cgctggtctc
     4261 cggcgcggtg ctcggcatct actcctcggg cctcaccctg ctgagcctcg gcatccgcat
     4321 cccgcggccg gccgccgccg gcatcgacgg cgtgatcctc accgccggaa cgatcttcgt
     4381 ggtgttcttc gcgaccagct tcatcggccc gttccagtcg ttcctcatca cgctcggcgt
     4441 gccgatggcg tcgtgggccg gcatcctcat cgccgacatc ctgcgccgac gtcgcgacta
     4501 cgacgaggcg gcgctgttcg actcgaacgg ccggtatggc gcgttcgact ggacctcgat
     4561 catcaccatg ctcgtcgcga ccggcatcgg ctggggcttc gtgatgaacc agatcccgga
     4621 caccccgtgg aacgactggc agggctattt cctcgacctg ttcggctggc gcgacgactg
     4681 gggctacgcc aacctcggcg tgctgttcgc gctggtgctg tcgttcgccg cgacctggtt
     4741 cgcccgtgcg gggaggatcc gccggcagga gcagggatga ccgaggctcc gatgagtctt
     4801 ggctgggtcg ctgagcccgt cgaagcgtcg ccgcactggt tggtcgtcat cgacccgcag
     4861 aacatcttcg cctctccgga ctcggagtgg gggtcgccgt tcttcgcgga cgccatggtg
     4921 aacatccgct ccctcgccga cgcccacggc gagcgggtgc tcgtcacgcg ctgggtgccc
     4981 accgccgatc gggcgacatc ctggggcgac tacttcgagg agtggccgtt cgcggacaag
     5041 ccggcatccg atccgctcta cgatctggtt ccggatgccg cggaactgtc tccgcatccc
     5101 acgctcgacc tgcccacctt cgggaagtgg ggcgccgagc tcgaggcgat cgtcgggcgc
     5161 ggcgcgcccg tggtgctggc cggggtctcg acggactgct gcgtgctctc cacggccctg
     5221 gcgggagcgg atgccggcgc acgcctcacc gtcgccacgg atgcctgcgc gggatcgacc
     5281 gccgagaacc acgcggccgc cctgcacgtg ctgggtctgt acccgccgca gatcgcactg
     5341 cggacgaccg cggagatcct gggcggctga accctccggg gggtcacgac gtccaggtgt
     5401 gcacgggctc gcccgtgtgc atccgctcca ggtattccgc gagcatgccg tgcagcgcct
     5461 cggatcgcgt ctcgcctgcg gcctcgcggg cggccaccgc gccccgctgc caggtg
//
LOCUS       DATMSX010000342         8041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166130,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000342 DATMSX010000000
VERSION     DATMSX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166130"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VN041_10660"
     CDS             <1..205
                     /locus_tag="VN041_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HWU29534.1"
                     /translation="PAVAGLALFGAFGSAVQQAIDDPGERMPAVITFLVAASGVALLG
                     ISAAFWALIAGLVVRGVLHLGRP"
     gene            291..1184
                     /locus_tag="VN041_10665"
     CDS             291..1184
                     /locus_tag="VN041_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU29535.1"
                     /translation="MTGELHLEGISKSYGSRTVLDDIRFDVAPGRLTGFVGGNGAGKT
                     TTMRIILGVLASDGGTVTLDGAPVTTADRRRFGYMPEERGLYPKMKVLEQVVYLARLH
                     GFGKADATVRATALLQELGLGERLDDLIESLSLGNQQRAQIAAALVHDPEVLILDEPF
                     SGLDPLAVDVVADVLQRRAAQGASILFSSHQLDVVERLCDDLIIIAGGTIRAAGTREQ
                     LRAQHSTLRYQLDSAGDAGWLRGEPGVTVIDFEGGSALFDVDTPDTAQRVLREAVGRG
                     EVSNFAPQHPSLAQIFKEVIR"
     gene            1181..2287
                     /locus_tag="VN041_10670"
     CDS             1181..2287
                     /locus_tag="VN041_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29536.1"
                     /translation="MSTQAPSATQPSKNTSSLVWLVAEREIGSKLRSKAFLISTGILL
                     LVALAGVLIGGFSSKSPTPDTIAATSQTANVVEALPNVKVTEVADRAAAEKLVRDDEV
                     TAAVLPGDGPTGVVVLAKKDAPNSLVMSLSVSPKVEILEPATTNSLLRYLVAIGFGVV
                     FLMAASTFGATIAQSVVEEKQTRVVELLISAIPTRVLLAGKVLGNTILAMAQIVLLAA
                     IAVIGLIVTGQNEVLAGIGAPVLWFAVFFLFGFILLASLYSGAAAMVSRQEDIGSTTM
                     PLMMLVMAPYILVIVFNDNPLVMTILSYIPFSAPVAMPVRLFVGEAQWWEPLLSLVLL
                     LAACVGAILVGAKIYENSLLRMGARVKLGEALRG"
     gene            2428..3015
                     /locus_tag="VN041_10675"
     CDS             2428..3015
                     /locus_tag="VN041_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWU29537.1"
                     /translation="MSTPVLDRTAQTEHPVLDVLADRWSPRAFDTEAPIDEAKLASAL
                     EAARWSPSANNSQPWRFIVARRGTALHEGIHAALMGFNQAWAGNAAVLIVAVAETATE
                     DGTPITHAVYDLGQSIAHLSVQAHHDGLLVHQMSGFLPEQIRELAGLEERFAPMTVLA
                     LGELGDIDALPEVLAQRETAPRVRRPIGDTVLLSA"
     gene            complement(3130..4920)
                     /locus_tag="VN041_10680"
     CDS             complement(3130..4920)
                     /locus_tag="VN041_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWU29538.1"
                     /translation="MRIEDYALLSNCRTAALVSREGSIDWLCLPRLDSASTFGGLLGD
                     DSHGRWSLRPSDPDATATRRYDEDTFVLITRWESADAVAEVHDFMPIDPDAGVDIRRT
                     DVVRRVVGVQGTMHLEQRISIRFDYARAMPWVRQTGTAHHPQLVAMAGPDALALRGAA
                     VKAVDHEHRGDLTVADGEVRDLQLTWFPSHLDVPEPLDVDAELERTKAWWQGWADTIE
                     HEGRHHDVVVRSLLILRALTNHETGGIAAAATTSLPEDFGGERNWDYRYVWLRDAALT
                     LEALLDHGFLALADRWREWLLRAVAGDPADLQIMYGLAGERDLTERELDWLPGFAASA
                     PVRIGNGAAAQYQADVVGEVLVTLSAARSAGLEESKFSWPLERQLLRFASEQLERPDQ
                     GLWEIRGDPHLFTHSRVMMWAAFDRGVRAVEEFGLDGPVQRWRELRDRLCAEIDRGGV
                     TDGYFTQYYGSSEVDASLLLLPQVGFCAPDDPRMLKTVERIEQTLMHDGLLHRYRTGT
                     GVDGLAGDESPFLACSFWLVEQYAVTGREDEAQELMDRLCALRNDVGMLSEEYDPVRE
                     RQVGNTPQAFSHLALVRAADALMRTEHRPR"
     gene            5002..6369
                     /locus_tag="VN041_10685"
     CDS             5002..6369
                     /locus_tag="VN041_10685"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWU29539.1"
                     /translation="MPTTTLYILGASGDLTSRLLLPSLASLLCKEPKRRVRLRGSGTE
                     DWDAGTWRSAVEKAFADSPQALEHVDIADYVKADVTDADDVASLVKGLSPSTVLYFAL
                     PPAVTAAAIDAMEGLTLPEGTILAMEKPFGEDEASARELNGKLLALVPEQQIFRVDHF
                     LGRSTLLNLLGVRLANRIWEPVWRAEHVQQVLIRFDESVALEGRARYYDKAGAMIDMI
                     QSHLLQVMALVAMDPPSTLGERDLRDAKAAVLRATHVMGDDPATASRRAQYTAGTQGD
                     RTLPSYRDEEGVDPDRQTETLAEVTFEIASDRWAGVPFVLRSGKALDRKHSEIVVTFK
                     PVRHVPPGLNGTPVDGGRLTFSLGPDEMHLRLHVTGGDDPFALRHEDLVADLGEGQQR
                     SYEEVLDELLDGDVALSVRADEAEECWRIIQPIRDAWAAKAVPLEEYPAGSTGPDAWA
                     TSHES"
     gene            6462..7775
                     /locus_tag="VN041_10690"
     CDS             6462..7775
                     /locus_tag="VN041_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29540.1"
                     /translation="MTALPRRTRAGYALGGIASGTYGTVPGLILMPFLTDLLGVQAAI
                     AGMIVFAPKAWDFFLNPLAGRISDRSRHPRDRRRPFILRAGILLAIAFLVMFFGPTAP
                     PVAAALWVLLLFLTTATVYAFFQVPYLAMSAEITDDYDERTRLITWRVIVFTLAILVS
                     GAAAPLLVETFGGATGYRVMAGAMAVLILIGAVGVWWGTRGVPLTRSETAGGRLFEQL
                     AVVLRNRDARMLILTFVMQAVAIGMVLGGVVYVARHVTNDPGIATLAFVCFVAPAVVL
                     TPAWSALARRIGKRRGFAVATVVETIGFIGLLGARSGEAVPLLVAAGIVGIGYAGGQL
                     FPLAMLPDLAADDAKRSGTNRIGMISGVWSGFELLGYALGPALLGLALSVGGYVASTG
                     DGVAQSGTAQWAIVLGVSVIPAVLCAISLIPLSRYRLDDAMRAAA"
     gene            complement(7782..>8041)
                     /locus_tag="VN041_10695"
     CDS             complement(7782..>8041)
                     /locus_tag="VN041_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29541.1"
                     /translation="ADDVDDAAVRAQHTAQTHRVGVLVGPAPLFVCVRARTLTALTGI
                     ERDWGEGIEVVDRWTVLRGIKRNGHLLDAAGRTRGRVPLAW"
BASE COUNT         1140 a         2939 c         2728 g         1234 t
ORIGIN      
        1 cccggcggtg gccggtctcg ccctgttcgg cgccttcggc tcggccgtgc agcaggccat
       61 cgacgacccg ggcgagcgga tgcccgccgt gatcacgttc ctggtggccg cgtccggcgt
      121 cgcgctcctc ggcatcagcg cggcgttctg ggcgctgatc gccgggctcg tcgtgcgcgg
      181 cgtcctgcac ctcgggcggc cctgacgccg ccggctccgc cgcggggatg acgacgtcaa
      241 tccaccgcag gggggatgct cccacccggc gcggttcgta gcgtggaggc atgacaggcg
      301 aactgcacct cgagggcatc tccaagagct acggctcacg gaccgtgctc gatgacatcc
      361 gcttcgacgt cgcgccgggc cggctgaccg gcttcgtcgg cggcaacgga gccggcaaga
      421 cgaccaccat gcgcatcatc ctgggcgtgc tggcctcgga cggcggcacc gtcaccctcg
      481 acggcgcacc cgtgaccacc gccgaccgcc gtcggttcgg gtacatgccc gaggaacgcg
      541 gcctgtaccc gaagatgaag gtgctcgagc aggtggtcta cctcgcccgt ctgcacggct
      601 tcggaaaggc ggatgccacg gtgcgcgcca ccgcgctgct gcaggagctc gggctcggcg
      661 agcgcctgga cgacctgatc gagtcgctgt cgctgggcaa ccagcagcgc gcgcagatcg
      721 ccgcggcgct ggtgcacgac cccgaggtgc tcatcctgga cgagccgttc tccggcctgg
      781 acccgctcgc ggtcgacgtg gtcgccgacg tcctgcagcg ccgggccgcg cagggcgcct
      841 ccatcctgtt ctcctcgcac cagctcgatg tcgtcgagcg gctgtgcgac gacctgatca
      901 tcatcgccgg gggcacgatc cgcgccgccg gaacccgcga gcagctgcgc gcccagcact
      961 ccacgctgcg ctaccagctc gactccgcgg gagacgcggg ctggctgcgc ggcgagcccg
     1021 gcgtcacggt catcgacttc gagggcgggt cggcactgtt cgacgtcgac acccccgaca
     1081 ccgcgcagcg cgtgctgcgc gaggccgtcg gccgcggcga ggtttcgaac ttcgcacccc
     1141 agcatccgtc cctcgcccag atcttcaagg aggtcatccg atgagcacgc aggcgccctc
     1201 ggcgacgcag ccgagcaaga acacctcgtc cctggtctgg ctggtcgccg agcgcgagat
     1261 cggctccaag ctgcgcagca aggccttcct gatctcgacc ggaatcctgc tgctggtcgc
     1321 tctggccggc gtgctgatcg gcgggttctc cagcaagtcg ccgacacccg acacgatcgc
     1381 cgcgacctcc cagaccgcga acgtcgtgga ggcgctgccg aacgtgaagg tgaccgaggt
     1441 cgccgaccgc gcggccgccg agaagctcgt gcgcgatgac gaggtcaccg cggcggtgct
     1501 ccccggagac ggcccgaccg gcgtcgtcgt cctcgccaag aaggacgccc cgaacagcct
     1561 cgtcatgtcg ttgtccgtct cgccgaaggt ggagatcctc gaacccgcca cgacgaactc
     1621 cctgctgcgc tacctggtcg cgatcggatt cggggtcgtg ttcctgatgg cggcgtccac
     1681 gttcggggcg acgatcgccc agagcgtcgt ggaggagaag cagacccgag tcgtcgagct
     1741 tctgatctcg gcgatcccga cccgcgtgct gctggccggg aaggtgctcg gcaacaccat
     1801 cctcgcgatg gcgcagatcg tgctgctcgc ggcgatagcg gtgatcgggc tgatcgtcac
     1861 cgggcagaac gaggtgctcg ccggcatcgg cgcacccgtg ctgtggttcg cggtcttctt
     1921 cctcttcggc ttcatcctgc tcgcgtcgct gtactccggc gccgcggcga tggtctcgcg
     1981 gcaggaggac atcggctcga cgacgatgcc gctgatgatg ctcgtgatgg ccccgtacat
     2041 cctggtgatc gtcttcaacg acaacccgct ggtgatgacg atcctgtcgt acatcccgtt
     2101 ctcggctccg gtcgcgatgc cggtgcggct gttcgtcggc gaggcgcaat ggtgggagcc
     2161 gctgctgagc ctggtgctgc tcctggccgc ctgcgtgggc gcgatcctgg tgggcgcgaa
     2221 gatctacgag aactcgctgc tgcggatggg cgccagggtc aagctcggcg aggcgctgcg
     2281 cggctgacgg atgcttcgac aggctcagca ccccagtcgg gcgctgagcc tgtcggcatg
     2341 tcgggaatat gcacgcgcat agagtggttg tggcaccagg acgatgaggt ccgacagaat
     2401 ccgcaccacc cgaaaggcct catccagatg agcactcctg tcctcgaccg caccgcccag
     2461 accgagcacc ccgtcctcga cgtactggcc gaccgctgga gcccccgcgc gttcgacacc
     2521 gaggccccga tcgacgaggc caagctcgcc tcggccctcg aggccgcccg ctggtcgccc
     2581 agcgcgaaca actcccagcc ctggcgcttc atcgtggccc gccgtggcac ggcgctgcac
     2641 gagggcatcc acgccgcgct gatgggcttc aaccaggcct gggccggcaa cgcggccgtg
     2701 ctgatcgtgg cggtcgccga gacggccacc gaggacggca cccctatcac ccacgccgtc
     2761 tacgacctcg gccagtcgat cgcgcacctc tcggtgcagg cgcaccacga cggcctgctc
     2821 gtgcaccaga tgagcggctt cctgcccgag cagatccgcg agctcgccgg cctggaagag
     2881 cgcttcgccc ccatgaccgt cctcgccctg ggcgagctcg gcgacatcga tgcactgccg
     2941 gaggtgctcg cgcagcgcga gaccgccccg cgcgtgcgcc gtccgatcgg cgacacggtg
     3001 ctgctcagcg cctgacccgc ttccgggcac gccccagacc aggaggatcg ccgagaacag
     3061 gatgctctgc gcagcatccg tcctgttctc ggcgatcctc ctgttgtcag cgacgcgatg
     3121 cgcctcgcgt caccgcggcc ggtgctccgt cctcatcagg gcatcggccg cgcgcaccag
     3181 ggcgaggtgc gagaacgcct gcggggtgtt cccgacctgg cgctcccgca ccgggtcata
     3241 ctcctccgac agcatcccga catcgttccg cagcgcgcac aaccggtcca tcagctcctg
     3301 cgcctcgtcc tcgcggcccg tcaccgcata ttgttcgacg agccagaacg agcaggccag
     3361 gaacgggctc tcatcccccg cgaggccgtc gacgcccgtg ccggtgcgat agcgatgcag
     3421 gagtccgtca tgcatcagcg tctgctcgat gcgctcgacg gtcttcagca tccgcgggtc
     3481 atccggcgcg cagaacccca cctgcggcag cagcagcagc gacgcgtcca cctcactgct
     3541 gccgtagtac tgggtgaagt acccgtccgt cacgcccccg cggtcgatct cggcgcacag
     3601 ccgatcgcgc agttcacgcc agcgctgcac cggcccgtcc aggccgaact cctcgaccgc
     3661 gcgcacgccg cggtcgaacg cggcccacat catcacgcgg gagtgcgtga acaagtgcgg
     3721 gtcgccgcgg atctcccaca gcccctgatc cggccgttcg agctgctccg aggcgaagcg
     3781 cagcagctgc ctctccagcg gccaggagaa cttcgactcc tccagccccg ccgaacgcgc
     3841 agccgagagc gtcaccagca cctcgcccac cacatcggcc tgatactgcg cggcggcgcc
     3901 gttgccgatg cgcaccggcg cggatgccgc gaagccgggc agccagtcca gctcgcgctc
     3961 ggtcagatcg cgctccccgg ccagtccgta catgatctgc aggtcggccg ggtctcccgc
     4021 gaccgcccgc agcagccact cccgccacct gtcggcgaga gcgaggaagc cgtggtcgag
     4081 cagcgcctcc agcgtgagcg cagcatcccg cagccacacg tagcggtagt cccagttgcg
     4141 ctcgccgccg aagtcctcgg gcagtgacgt cgtcgccgcc gcggcgatgc cgcccgtctc
     4201 gtggttggtg agcgcacgca ggatcagcag cgaccgcacg accacgtcat ggtgccggcc
     4261 ctcgtgctcg atcgtgtcgg cccacccctg ccaccacgcc ttggtgcgct ccagctcggc
     4321 gtccacgtcg agcggctccg gaacgtccag gtgcgagggg aaccatgtca gctgcagatc
     4381 ccgaacctcc ccgtccgcga ccgtgaggtc gccccggtgc tcatgatcca cggccttcac
     4441 cgccgcgccg cgcagcgcga gggcatccgg ccccgccatc gccaccagct gcgggtggtg
     4501 ggcggtgccg gtctgccgca cccacggcat cgccctcgca tagtcgaagc ggatgctgat
     4561 ccgctgctcc aggtgcatcg tgccctgcac gccgacgacc cggcggacga cgtcggtgcg
     4621 ccggatgtcg acgccggcgt cggggtcgat gggcatgaag tcgtgcacct ccgccacggc
     4681 atccgcgctc tcccaccgcg tgatcagcac gaaggtgtcc tcgtcgtagc ggcgcgtcgc
     4741 cgtggcatcc ggatcggagg ggcgaagcga ccacctgccg tgcgagtcgt cgccgagaag
     4801 cccgccgaag gtgctggcgg agtccagacg cggaaggcag agccagtcga tgctcccctc
     4861 cctcgacacc agcgccgcgg tgcggcagtt gctcagcagt gcatagtcct cgatccgcat
     4921 ggccacgccc gcagtctcgc actcgcgcca tgccgaatcc agcctcttgc tttcagggga
     4981 cgtgggacta gcgtcgatct catgccgacc accacgctct acatcctcgg cgcctccggc
     5041 gacctcacct cccgtctgct gctgccctcg ctggcgtcgc tgctgtgcaa ggaaccgaag
     5101 cgccgcgtcc ggctgcgcgg ttcgggcacg gaggactggg atgccggcac ctggcgctcg
     5161 gccgtcgaga aggcgttcgc cgattcgccg caggcgctgg agcacgtcga catcgccgac
     5221 tacgtcaagg ccgacgtgac ggatgccgac gacgtcgcct cgctggtgaa gggcctcagc
     5281 ccctcgacgg tgctctactt cgcactgccg ccggccgtca ccgcagccgc gatcgacgcc
     5341 atggagggtc tcacgctgcc ggagggcacg atcctggcga tggagaagcc gttcggcgag
     5401 gacgaggcct cggctcgcga gctgaacggc aagctgctcg ccttggtgcc cgagcagcag
     5461 atcttccgcg tggaccactt cctgggccgg tcgacgctgc tgaacctgct gggcgtgcgc
     5521 ctggcgaacc gcatctggga gccggtgtgg cgcgccgagc acgtgcagca ggtgctgatc
     5581 cgcttcgacg agagcgtggc tctcgagggc agggcccggt actacgacaa ggccggcgcc
     5641 atgatcgaca tgatccagag ccacctgctg caggtgatgg ccctggtcgc gatggatccg
     5701 ccctccaccc tcggcgagcg cgacctgcgc gacgcgaagg cggccgtcct gcgcgccacg
     5761 cacgtgatgg gcgacgaccc ggcgaccgcg tcgcggcgcg cccagtacac ggcgggcacg
     5821 cagggcgacc gcacgctgcc ctcctaccgc gacgaagagg gcgtcgaccc ggaccggcag
     5881 accgagacgc tggccgaggt caccttcgag atcgccagcg accgctgggc gggcgtgccg
     5941 ttcgtgctcc gctccggcaa ggccctggac cgcaagcact ccgagatcgt ggtgaccttc
     6001 aagcccgtgc ggcacgtgcc gccggggctg aacggcactc ccgtcgacgg cggcaggctg
     6061 acgttctcgc tcgggcctga cgagatgcat ctgcgcctgc acgtcaccgg cggcgacgat
     6121 ccgttcgcgc tgcggcatga ggacctggtc gccgacctcg gcgaaggcca gcagcggtcc
     6181 tacgaggagg tcctggacga actgctggac ggggacgtgg cgctctcggt gcgcgccgac
     6241 gaggccgagg agtgctggcg gatcatccag ccgatccggg atgcctgggc ggcgaaggcc
     6301 gtgccgctgg aggagtatcc cgccggttcg accgggccgg atgcctgggc cacctcgcac
     6361 gagtcctgac agacggcgga tcgccccatc cacaggggca gcgctgacgg aatgagcgca
     6421 tccgtcctcc ccggatgacg gacgccccta cgatcggctc catgacagcg cttccgcgcc
     6481 gaacccgcgc cgggtacgcg ctgggcggca tcgcctcggg cacctacggc accgtgcccg
     6541 ggctgatcct gatgcccttc ctcaccgacc tgctcggcgt gcaggcggcg atcgccggca
     6601 tgatcgtctt cgcgccgaag gcgtgggact tcttcctcaa cccgctcgcg ggccgcatca
     6661 gcgatcggtc acggcatccg cgcgaccggc ggcggccgtt catcctgcgc gccggcatcc
     6721 tgctcgcgat cgccttcctg gtgatgttct tcgggcccac ggcgcccccg gtggccgcgg
     6781 cgctgtgggt gctgctgctg ttcctgacca ctgccaccgt ctacgcgttc ttccaggtgc
     6841 cgtatctggc gatgtcggcc gagatcacgg acgactacga cgagcgcacc cggctgatca
     6901 cctggcgggt gatcgtgttc acgctcgcga tcctcgtgtc gggtgccgcg gccccgctgc
     6961 tcgtggagac gttcgggggc gcgaccgggt atcgcgtcat ggcgggcgcg atggccgtgc
     7021 tgatcctgat cggcgcggtc ggcgtgtggt ggggaacgcg cggtgtgccc ctgaccagga
     7081 gcgagaccgc cggcggtcgg ctcttcgagc agctcgcagt ggtgctgcgc aaccgcgatg
     7141 cacggatgct gatcctcacc ttcgtgatgc aggccgtcgc gatcgggatg gtgctcggcg
     7201 gagtcgtgta cgtggcccgc cacgtgacga acgatccggg gatcgccacc ctcgcgttcg
     7261 tctgcttcgt cgctcccgcg gtcgtgctca cgccggcgtg gtcggcgctg gcgcgtcgca
     7321 tcggcaagcg ccgcggcttc gcggtcgcca cggtcgtgga gaccatcgga ttcatcgggc
     7381 tgctcggcgc ccgaagcgga gaggcggtgc cgctgctggt cgccgcgggg atcgtgggca
     7441 tcggctacgc cggcgggcag ctcttccccc tggcgatgct gcccgacctc gccgctgacg
     7501 atgcgaagcg gagcggcacc aaccggatcg ggatgatctc gggcgtctgg tccggcttcg
     7561 aactgctcgg ctacgcgctg ggaccggcgc tgctgggact ggcgctcagc gtcggcgggt
     7621 acgtcgcgtc aaccggcgac ggcgtcgccc agtccggcac cgcgcagtgg gcgatcgtac
     7681 tgggcgtatc ggtgatccct gcagttctgt gcgcgatcag tctcatcccg ctgtcgcgct
     7741 accgcctgga cgatgcgatg cgcgcggcgg cgtgagcgcg ttcaccaggc cagcggcacg
     7801 cgtccgcggg tgcgccccgc cgcgtcgagc aggtggccgt tgcgcttgat cccgcgcagc
     7861 accgtccatc gatcgaccac ctcgatgccc tcaccccagt cgcgctcgat tccggtcaga
     7921 gccgtcagcg tgcgggcccg cacgcacacg aacagtggtg cggggcccac cagcacgccc
     7981 acccggtggg tctgcgcggt gtgctgggcg cgcacagcgg cgtcgtcgac gtcgtcggcc
     8041 c
//
LOCUS       DATMSX010000343         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_5660,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000343 DATMSX010000000
VERSION     DATMSX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5660"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VN041_10700"
     CDS             <1..633
                     /locus_tag="VN041_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWU29542.1"
                     /translation="ATSALRGEDERERVLAVAFRLLDRTAIRIGSPGYLRRYGSRGLT
                     TLRWADVTWRDTTITLSFRGKMNLIHTIEFDDEDLIAWIVSHPAHPPRAFVLRYRDGS
                     RRRAIGPADVNAFLAEAAGEPFTAKDFRTLHGTIVAATELARIGPRDTDSARRRAVSA
                     AMRATAAALGNTPAVARSGYVDPRVLDLYDDGIVVDTGRRVEPQLGELLA"
     gene            732..974
                     /locus_tag="VN041_10705"
     CDS             732..974
                     /locus_tag="VN041_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29543.1"
                     /translation="MSDAQRGSTTHGPNLDDQLEQESRGMVQGHGAPHAESFRETEPL
                     PDDTDSPEVRQAFEREDPGADAENPYDEEDDDDKLA"
     gene            958..2319
                     /locus_tag="VN041_10710"
     CDS             958..2319
                     /locus_tag="VN041_10710"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HWU29544.1"
                     /translation="MTSSLDPDGLDLHEALSTPGAWTTVYTDGPSGEPPGAAESRMRS
                     LRDRMQEAGVPEEDADAVVAALPEDTGVPAPSARWLLVKDGSVVADEGFAAPRRGAER
                     ISHGTFPEIVPLLRHRTGNPLILVVATGRDGAEITLERLGRAGSEKVEQVEGDEYPIT
                     KSSPGGWSQARFQRSVEEVWLRNQTEIGGIVDEITRTRHPDHIFVTGDAHARGLLLES
                     IGADTLDRVVEVDVITRAAGADDEALTAAIDETVAAAHDEQVASVRDRAAERDARNGV
                     SGIGSVVAALQQAQVETLLLDARLADTDASVLALRETPWVAASPADNFDAGPGTPVSA
                     AEGLARAAVLTDAEIVFIEDEPAEGEPRREGAAAAPLAVLRWSTESGSDDLEAPVREA
                     SSADPGTPASDSATGADPGADGHPSQAEGEDPDEDVAHHDPRTSGHPSQAEGEDPADD
                     ERA"
     gene            2348..3148
                     /locus_tag="VN041_10715"
     CDS             2348..3148
                     /locus_tag="VN041_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29545.1"
                     /translation="MTDILSSLTNLPAPQFIEVGDGVAVATYAWGDPDAPVVVLVHGF
                     ASSTADTWLHTGWVRMLEREGYRVLGIDQRGHGASQKPHDSAGYAVANLARDVEAVLD
                     THLVEDALYVGYSLGARVGWQVLQDLGDRIPRGVLGGVPDGIPLDRLDVDQVCRYADD
                     GIPVTDPVTQSYIQLAERVPGNDLYALLALAEGMRASRSIDPDPAHAPQQPVLFATGS
                     EDAVIEGSRRLAEAAPQATFFTIPGRHHFNAPGSKDFRLAALEFLQAE"
     gene            complement(3166..3345)
                     /locus_tag="VN041_10720"
     CDS             complement(3166..3345)
                     /locus_tag="VN041_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29546.1"
                     /translation="MTKSTIWSILGAILALVIAWWIVSALFSLLWFVAKLALVVVVAL
                     VVYAGIRALTRDARS"
     gene            complement(3419..3985)
                     /locus_tag="VN041_10725"
     CDS             complement(3419..3985)
                     /locus_tag="VN041_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU29547.1"
                     /translation="MQTLTIDFIMSLDGYGAAEGWPGWWGLESAEYLDWLAGHPDRDA
                     PLLMGATTYRLMSAFAAQGSEGVDSLDAVQKYVFSSSLDEPLDWAHSTLVPGDAVEFV
                     RELKETSDRPLRTLGSISLCRSLLNAGLVDRYRVVVFPVITGKTGQDRIFDAYPDVLL
                     ELVEARTFDHRTQLLEYIPTVLDGPPGS"
     gene            4141..>4288
                     /locus_tag="VN041_10730"
     CDS             4141..>4288
                     /locus_tag="VN041_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWU29548.1"
                     /translation="MFEGPLSASAYEVLGVAASAPDDELRRAYRLRLRQTHPDTGGDA
                     ALFIR"
BASE COUNT          675 a         1525 c         1527 g          561 t
ORIGIN      
        1 gcgacgagcg cgctgcgcgg cgaggacgag cgcgagcggg tcctcgccgt cgcgttccgc
       61 ctcctcgatc gcacggcgat ccggatcgga tcccccgggt acctgcgccg ctacggcagc
      121 cgcgggctca cgacgctgcg atgggccgac gtcacctggc gagacacgac catcaccctg
      181 tcgttccgcg gcaagatgaa cctcatccac acgatcgagt tcgacgatga ggatctcatc
      241 gcctggatcg tctcgcatcc ggcgcatccg ccgcgcgcgt tcgtgctccg ctaccgcgac
      301 gggtcacggc gtcgcgcgat cgggccggct gacgtgaacg cgttcctcgc cgaagccgca
      361 ggcgaaccgt tcaccgcgaa ggacttccgc acgctgcacg gcaccatcgt cgccgccacg
      421 gagctggcgc ggatcggccc ccgcgacacc gacagcgcac gccgccgagc cgtctctgcg
      481 gccatgcggg cgaccgccgc cgctctgggg aacactcccg cagtcgcgcg gtcgggctat
      541 gtcgacccgc gcgtgctcga cctctacgac gacggcatcg tcgtggacac cggtcgtcga
      601 gtcgagccgc agctcgggga gctgctggcc tgacccgagg gcacggaaca cgggcatccg
      661 tcctgtcaac ccggtgtcga gcgccgacct ccccgcctaa cgtcgcccgc ggacccgatg
      721 aaggaggaga gatgagcgac gcacagcgcg gcagcacgac gcacgggccg aacctcgacg
      781 atcagctcga acaggaatcc cgtgggatgg tgcagggcca cggcgccccg cacgcggagt
      841 cgttccgcga gaccgagccc cttccggacg acaccgactc tccggaggtg cggcaggcgt
      901 tcgagcggga ggatcccggc gccgacgccg agaaccctta cgacgaggag gacgacgatg
      961 acaagctcgc ttgaccccga cggcctggac ctgcacgagg cgctcagcac tcctggcgcc
     1021 tggacgaccg tgtacaccga cggaccttcg ggcgagccgc ccggcgcggc ggagagcagg
     1081 atgcgctcgc tgcgggaccg gatgcaggag gcgggagtac ccgaggagga cgcggatgcc
     1141 gtcgtcgccg cgctgcccga agacaccggc gtgcccgcgc cgtcggcgcg ctggctgctc
     1201 gtcaaggacg gttcggtcgt cgccgacgag ggcttcgcag ccccgcggcg cggcgccgag
     1261 cgaatctcgc acgggacgtt ccccgagatc gtcccgctgc tgaggcaccg gaccggcaac
     1321 cctctcatcc tggtggtggc gacggggcgc gacggcgccg agatcactct ggagcgtctc
     1381 ggccgcgccg gatcggagaa ggtggagcag gtcgaaggcg acgagtaccc gatcaccaag
     1441 tcgagccccg gcgggtggtc gcaggcccga ttccagcgca gtgtcgaaga ggtgtggctg
     1501 cgcaaccaga ccgagatcgg cgggatcgtc gatgagatca cgcgcacccg gcaccctgac
     1561 cacatcttcg tcaccggcga cgcgcacgcc cggggcctgc tcctcgagag catcggcgct
     1621 gacacgctcg accgggtggt ggaggtggat gtcatcaccc gcgcggcggg ggctgacgac
     1681 gaggcgctga ccgccgcgat cgacgagacg gtcgccgccg cacacgacga gcaggtcgca
     1741 tcggtgcggg atcgcgccgc cgaacgcgat gcgcgcaacg gcgtgtccgg catcggctcg
     1801 gtggtcgccg cgctgcagca ggcacaggtc gagactctgc tgctggatgc acggctggcc
     1861 gacaccgatg cctcggtgct cgcgctgcgc gagacaccgt gggtcgcagc gagcccggcc
     1921 gacaacttcg acgcggggcc gggcacaccg gtgtcggcgg cggagggcct ggctcgtgcc
     1981 gccgtgctga ccgatgccga gatcgtgttc atcgaggatg agccggcgga gggtgagcca
     2041 cggcgcgaag gcgcagcggc cgcaccgctg gccgtgctcc gctggtccac cgagtccggc
     2101 agcgatgacc tcgaggctcc ggtccgcgaa gcatcctccg cggaccccgg caccccggca
     2161 tccgactccg ccaccggtgc ggacccgggc gcggacggcc atcccagcca ggccgaggga
     2221 gaggatcccg acgaggacgt cgcgcatcac gacccgcgca cgagcgggca tccctctcag
     2281 gcggagggcg aggacccggc ggacgacgag cgcgcctgac cggcgggaac agggccaaga
     2341 tggtgatgtg accgacatcc tctcctcgct gacgaacctg cctgctccgc agttcatcga
     2401 ggtgggtgac ggcgtcgcgg tggcgacgta cgcgtggggc gatccggatg cacccgtggt
     2461 cgtgctcgtg cacggcttcg catccagcac cgccgacacc tggctgcaca ccgggtgggt
     2521 gcggatgctg gagcgtgagg gctaccgcgt cctgggtatc gatcagcgag gccacggggc
     2581 gagccagaag ccgcacgatt ccgcgggtta cgccgtggcg aacctggcgc gcgacgtcga
     2641 ggccgtgctc gacacgcacc tcgtcgagga cgcgctctac gtcggctact ccctcggcgc
     2701 ccgcgtcggc tggcaggtgc tgcaggatct cggcgaccgc atcccccgcg gtgtgctggg
     2761 cggcgtgccc gacggcatcc ccctggaccg actcgacgtg gaccaggtgt gccggtacgc
     2821 cgacgacggc atccccgtca ccgacccggt cacgcagagc tacatccagc tggccgagcg
     2881 ggttccgggc aacgacctgt acgcgctgct cgcactggcc gagggcatgc gcgcatcgcg
     2941 ctcgatcgac ccagatcccg cccacgcgcc gcagcagccc gtgctcttcg ccaccggctc
     3001 ggaggacgcg gtgatcgagg gctcacgccg cctggccgag gccgcaccgc aggcgacctt
     3061 cttcacgatc cccggccggc accacttcaa cgcgccggga tcgaaggact tccggctcgc
     3121 ggcgctggag ttcctgcagg cggagtgatc ccgacgggcg agtggtcagc tgcgcgcgtc
     3181 gcgggtcagg gcgcggatcc ccgcgtagac cacgagcgcg accacgacca ccagcgcgag
     3241 cttggcgacg aaccacagca gcgagaacag cgcgctgacg atccaccacg cgatgacgag
     3301 ggcgaggatg gcgccgagga tgctccagat cgtgctcttg gtcatcgtcc cagcgtagag
     3361 gagtcccgtg atggcgccgg atgcgctcag ccacgggtgc ctcagcttcg ggatgcgctc
     3421 agcttccggg aggcccgtcg agaacggtcg ggatgtactc gagcagctgg gtgcgatggt
     3481 cgaaggtgcg cgcctcgacc agctcgagca gcacatcggg gtacgcgtcg aagatccggt
     3541 cctgtccggt cttccctgtg atcaccggga agaccactac gcggtaccga tcgacgagcc
     3601 ccgcattcag cagcgagcgg cacagcgaga tactgcctag ggtccgcagc gggcgatcgg
     3661 aggtctcctt cagctcgcgc acgaactcga ccgcgtcgcc gggcacgagc gtggagtggg
     3721 cccagtccag cggctcgtcg aggctggacg agaacacgta cttctgcacg gcgtcgaggg
     3781 agtccacgcc ctccgagccc tgcgcggcga aggcggacat cagccggtag gtggtcgcgc
     3841 ccatcagcag cggcgcgtcc cggtcgggat gcccggcgag ccagtccagg tattcggcgc
     3901 tctccaggcc ccaccacccc ggccagccct cggcggcgcc gtagccgtcc agcgacatga
     3961 tgaagtcgat cgtcagagtc tgcatcggat cctcctcggc acgcggtcca cggcgagacg
     4021 gcacccgccg cacgccggtt cccggcagag cctacgctcc gccgtccggc cgggtaaccc
     4081 cgatctgcac cgtaggatct ggttgcgacg ggcagcccgt cgggatgccg gaggagggcg
     4141 atgttcgagg ggcccctgtc ggcatccgca tacgaggtgc tgggtgtcgc ggcgagcgcg
     4201 ccggacgacg agctgcgccg ggcgtaccgg ctgcggctgc ggcagaccca ccccgacacc
     4261 ggcggcgatg cggcgctgtt catccgcg
//
LOCUS       DATMSX010000344        13444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166154,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000344 DATMSX010000000
VERSION     DATMSX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166154"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VN041_10735"
     CDS             <1..404
                     /locus_tag="VN041_10735"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWU29549.1"
                     /translation="RAGADAGEPDALVHRALAQLTAYFAGELQAFDLPVDLGRQTDAT
                     RAVLMALHETVGHGETVTYGELAARSGTDVPARGIGAIMGANPIPLIVPCHRVVASDG
                     LGGYSGGRDGEGLLTKRWLLEHEGALPTALF"
     gene            413..1177
                     /locus_tag="VN041_10740"
     CDS             413..1177
                     /locus_tag="VN041_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU29550.1"
                     /translation="MTAYWENGAYGRGSSRRRQHPIVAVEPNDDAPPPSAQWPSSIPA
                     VAQLLREGIRLAPGVTFLVGENGSGKSTIVEGVAVAYGLSPEGGSRGARHSTRPTESP
                     LSDWLRIQRGVGASRWGFFLRAETMHSFYTYLEENPSPYDSQFHEMSHGESFLEILGT
                     RFDEPGFYCLDEPEAALSFGSTLTLISVLQRIADDGGQVLCATHSPVLAALPGAQILE
                     VGEWGLRPVAWDDLELVRHWRSFLSGPERYLRHLLD"
     gene            complement(1184..2086)
                     /locus_tag="VN041_10745"
     CDS             complement(1184..2086)
                     /locus_tag="VN041_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU29551.1"
                     /translation="MTTVATPAARAWEAERGRLIGIAYRMLGDFGHAEDVVSEVAIDA
                     LRTEQADAGSVRSWPAWLTTTCVRRSIDRVRSLAAVREDYTGHWLPEPVETSRLPEDA
                     VADREMLSLALLHLAEQLTPDARAAVVLHRAFGMPATEIAEILEKSPAAVRQLISRGE
                     RRLRLDEDASPPRATDPQALSTLVDAVQGGDLGALLGLLTDETILWTDGGGVVSATRN
                     PIFGAEKIARFYLGVQAKAAVFDPVHPFTLESIDVNGEPAFALTHSGRTDVLSLEFDE
                     QGRIRGIRQVCNPHKLTRVLAQVA"
     gene            complement(2107..2565)
                     /locus_tag="VN041_10750"
     CDS             complement(2107..2565)
                     /locus_tag="VN041_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU29552.1"
                     /translation="MTRIDMARTNKLGYAAVLGMEAYASKSVERRLYELIKLRASILN
                     GCGFCVDMHATDGAKRGIPQRTLHAVGAWQHAKNLFEPKELAALALTDAITKLGPDTV
                     TDEIWNAAAEHFDESELGAIVMAIATINVWNRIAIATEMQPPVDDRHPIV"
     gene            2705..4123
                     /gene="thrC"
                     /locus_tag="VN041_10755"
     CDS             2705..4123
                     /gene="thrC"
                     /locus_tag="VN041_10755"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWU29553.1"
                     /translation="MRYISTRGGMTPQPFSETLLEGLAPDGGLAVPEQLPQVEAETLE
                     RWRALTYPQLAAEVLGLFATDIPREDLARMTADAYADFPDAVVPLRSIGDGITLVGLS
                     EGPTLAFKDMAMQFLGQALDYVLERKDQVLNIVGATSGDTGSAAEHALRGKDRVSVFM
                     LSPQGRMSSFQRAQMYSLDDANVHNIAVEGVFDDCQNLVKKLAGDLEFKRAQHLGAVN
                     SINLGRIAAQVVYYFWAWLRVTDAVEPEFRSGFEVSFTVPSGNFGNILSGFFAKGMGV
                     PIRRLVLAANENNVLDEFFRTGVYRPRSAADTHATSSPSMDISKASNLERFIFELVGQ
                     DPSRVADFFRSIDEQGFVDLSAEQPRFEAEFGIVSGTSTHADRLATIRSVHEASGEII
                     DPHTADGVKVAREYVETDVPMLVLETAKPQKFAETITEAIGVELKFSAELQRMLAAPQ
                     HVTEMADDEQALRAFVAANALH"
     gene            complement(4059..4601)
                     /locus_tag="VN041_10760"
     CDS             complement(4059..4601)
                     /locus_tag="VN041_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HWU29554.1"
                     /translation="MNAVPAVTAPLLGSAVRVAVGVLWVLEGLEKYRAGFGAADILLV
                     ADGTASNPRTPWWFTPVRATMQALPDAFGLAIPALEVLLGLALIAGVLTRLVALGSIA
                     TLALYWGSDQLIAQYPLMMLLGAAVLAVPDSGRYGAAWIIARFGSDRDGAGGDRPRRL
                     SAGRSPRRRRAAPARRQPSR"
     gene            complement(4598..5050)
                     /locus_tag="VN041_10765"
     CDS             complement(4598..5050)
                     /locus_tag="VN041_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HWU29555.1"
                     /translation="MRKNTFTAAIPVAVIALGLTLAGCSSTPGSDMPSKAPMSQGSDS
                     GSTPMADPMTGSFEGLNGKTVSGMASVEDGTLTLSGFSSDEGPDLHVYLTSGTDEAAV
                     GAGKEIDAVAFDKQSQTFMLAGVDTSMYSYVVIHCDKAKAVFGAAKLS"
     gene            5190..5882
                     /locus_tag="VN041_10770"
     CDS             5190..5882
                     /locus_tag="VN041_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU29556.1"
                     /translation="MDSSRVVVVEDDPVVRGAVGDYLRARGYRLDAFADGVAAREAIG
                     AEIPDLVIVDRMLPGISGDELCRAIREGSDVPIIMLTALGEVEQRIEGFEAGADDYLA
                     KPFALRELELRVDALLRRSMRAPAPSAFTAGDFEIDAARRKARVRGTEIALTGREYEL
                     LVHLVRNSDRTISRDEILREVWGWSFGEPSTVTVHVRRLREKIEEDPREPRYLHTVWG
                     AGYRFSAKATEL"
     gene            5882..7048
                     /locus_tag="VN041_10775"
     CDS             5882..7048
                     /locus_tag="VN041_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU29557.1"
                     /translation="MAEMIPLGVVDVLLIAGTALAGTVVVAAIALILLRWARGGSMLV
                     QMLVLASVAAVSMAAAVLLISVEMYISPHDLSVLVWVIGTSLLFSLLAAWLVARLTRA
                     RLVRISEAVRRVGEGDVVAAEPHGLREFDDLSVELADVSERLHAARQELEQLDSARRR
                     FFAWISHDLRTPLTAVRALSEAIEDGASAQPERYAGEVRAQIDTMSRMVDDLFELSKL
                     TSGAVRLRTQQVELLDVVSDAVADVRLAAEQRGVTIVERGVGGHVLWADPHQLGRVVV
                     NLLTNAVRHAPRDSEILVTATELDEQRLVLGILDHGSGVATSDLDRMFEVGWREDAAR
                     PTTANDPVASGAGLGLAIARGLARAHGGDVYAEHTGEGFRMNVLMPVGDESTQR"
     gene            7235..8575
                     /locus_tag="VN041_10780"
     CDS             7235..8575
                     /locus_tag="VN041_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HWU29558.1"
                     /translation="MARMAHADDFALSRVTPEARKPWFGIALQRFGQVSALSQFLLGA
                     TLGFSMTFGEAVLAFLFGSIILEVIMCVVGFIGQREGLNTALLARWTGFGEIGASLVG
                     LAIGISLIGWFGIQSAISAQSLDALMPGALPIWAWSLIFGLAVTAIVAFGFIGMQWLA
                     NVTVPLFLILVGWSVIGELSRHDIGELLTSPAPGPTMSVWAGTGIVAGGLIVGAIITG
                     DMTRFNRSRADVVKQTVVGVSLGEFVIGLAGVLLAHAAGTGDIVAIVTSSVGFIGLFI
                     VITGTLKINDWNLYSSTLGLVNFISTAFGRNLHRVTTTVVLGVVGSVLAAVGILGQFT
                     SFLIVLSVAFPPIAGIMVAEYFIVRRWRGELDATRDSGTLPATAPRLVPATLIVWLVS
                     SLVGYFVTWGIPSLLSLFLSMVLYVIAGKLGLVRGVGVATTRQTSASDSQPVGA"
     gene            8607..10154
                     /locus_tag="VN041_10785"
     CDS             8607..10154
                     /locus_tag="VN041_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWU29559.1"
                     /translation="MHIGIDVGGTNTDAVLMDATRTLAGVKHSTTSDVTSGIVQAIED
                     LRANHPFEGDDIDAVMIGTTHFINALVQASRLAPTAALRLGLPATRALPPLVDWPEVL
                     VEATHARSYLAHGGYEFDGRPISPIDPEELRAHAEDMKANGIRSVAISSVFSPVNHDL
                     ESQAAEIIGEVLGDDVAISLSHEIGRIGLLERENATIINAALRELASEIVDGLTSAVR
                     AQGIEAPIFLSQNDGTLMDEEYVRRYPVATFASGPTNSMRGAATTSGLDDCAVIDVGG
                     TTADIGLLINGFPRETANEVKVAGIRTNFRMPDVLSLGIGGGSIVDEETAEVGPASVG
                     YRLATEALVFGGSTLTATDIAVAAGRADVGDASKVAHLDPAFVARVLERIADRVSEAV
                     DRMRTSPEPIAVVAVGGGSILLPDELPMFGTVHRPENYAVANAIGASIAQVGGEIDKV
                     YAIEPGRRDETIAAVRAEAVDKAIAAGAKPSTVSVIDFDEVPIPYLPGNATRIRAKAV
                     GDLDMGA"
     gene            10156..11274
                     /locus_tag="VN041_10790"
     CDS             10156..11274
                     /locus_tag="VN041_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWU29560.1"
                     /translation="MTWTITAEHIDGLARGAAVLGTGGGGDPYIGALLARQALAGGDV
                     TVVGLDEVPDDAVVLFVAMMGAPTVMVEKLPSLDEVVEPVRALGVHLGRPITHIACAE
                     VGGVNSTIPIAAAAALGLPLIDADGMGRAFPELQMVLPTLYGVTASPLAFSDEKGNTG
                     VLRTVDNAWTERIARVACVEMGCSVMISGFSMSGTQARDSLVSGSLSRCVEIGQAIVG
                     AREEKADPVAAAVALLNGRELFDGKVVDVQRATTTGFARGRARIDGDGDSSITLQFQN
                     EHLVAEKDGQVLATTPDLIMVLEGESGEPITTEALRYGQRVRVIAAPADERWHSAEAL
                     AMVGPGYFGYDMPAHRFDGTISVGHGAEAAGEAAGEAA"
     gene            11271..12371
                     /locus_tag="VN041_10795"
     CDS             11271..12371
                     /locus_tag="VN041_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWU29561.1"
                     /translation="MSWELTAADLPDLARGATLLGTGGGGDPYIGKMLVERVLGDRSI
                     TILDPDELADDLFVIPTAQMGAPTVMVEKIPAGTEPTLALRTLEKHLGRTADATMPIE
                     CGGINSMIPLVVAAETGLPVVDADGMGRAFPELSMETFAVYGVHGSPLALAGERGEQV
                     VIDTGDDDRQMEWLARGITIRLGGVGHIAEYAMTGADVRRTAVPRTISMALALGRAIR
                     LAREEHRSPFEAIGATLASTLYPHLRELHVGKVVDVERRTTEGFAKGRAVIAPLGDDG
                     ETLEISFQNENLIARRGGRLVAIVPDLICVVDHETAEPITTEGLRYGQRVRVLGISTP
                     AMMRTPEALVAFGPSAFGLQEDFVAVEELAGP"
     gene            12381..13415
                     /locus_tag="VN041_10800"
     CDS             12381..13415
                     /locus_tag="VN041_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWU29562.1"
                     /translation="MVSRLGQDDLTALARGYALLGSGGGGPTTMLELMLGGSAAWPID
                     VVDPSALDPATPCLGVAFVGSTMLLSERMPGIDPFGPLIAAVERWLGHEVPAVCSLEG
                     GGMNGLAPLTLAGTHIVVDADCTGRAVPGLDQMSLFVDRVPGIVVACDTGAGGVALVE
                     AARAIDVERVVRAAIIQAGGAGCAVFAGFTVGDLREHAISGHLAHALDLGRAFVAASG
                     RPLAELADALGARLVAEGRILSVSALADDPHVSAVEIAGAGGTVHRVITRSETLAVVS
                     DGQVVAAAPTVIVVVDAISREVLEVTELSLARNVAVFAIPAPAWWIASPGRVEHVRPS
                     AYGLAELDVA"
BASE COUNT         1933 a         4737 c         4662 g         2112 t
ORIGIN      
        1 gtcgggccgg agcggatgcc ggtgagcccg acgcgctcgt gcaccgggcg ctcgcgcagc
       61 tgacggcgta cttcgcgggg gagttgcagg cattcgacct gcccgtcgac ctcggccggc
      121 agaccgacgc cacccgtgcg gtgctgatgg cgctgcacga gacggtcgga cacggcgaga
      181 cggtcaccta cggcgagctc gccgctcgca gcggcaccga cgtgccggcg cgcggcatcg
      241 gcgcgatcat gggcgccaac cccatcccgc tcatcgtgcc ctgccatcgg gtcgtcgcca
      301 gcgacggcct gggtgggtac tcgggcggac gggacggcga gggactgctc accaagcggt
      361 ggctcctgga gcacgagggc gcgctgccga cagcgctgtt ctgaggctgc ggatgacggc
      421 gtactgggag aacggggcgt acgggagggg cagctcccgc aggcggcagc atccgatcgt
      481 cgccgtggag ccgaacgacg acgcgccgcc gccgtccgcg cagtggccgt cgagcatccc
      541 cgcggtggcg cagctgctgc gcgaggggat ccggctcgca cccggcgtca cgttcctcgt
      601 cggcgagaac ggcagcggca agtcgaccat cgtcgagggc gtcgcggtgg cctacgggct
      661 gtcgccggag ggcggatcgc gcggagccag gcacagcacc cggccgaccg agtcgccgct
      721 gtcggactgg ctgcgcatcc agcgcggcgt cggcgccagc cgatggggct tcttcctgcg
      781 tgccgagacg atgcactcgt tctacacgta cctggaggag aacccgtcgc cgtatgactc
      841 gcagttccac gagatgagtc acggcgagtc gttcctcgag atcctcggca cccggttcga
      901 cgagcccggc ttctactgcc tcgacgagcc cgaggcggcc ctgtcgttcg ggtcgacgct
      961 caccctgatc tcggtgctgc agcgcatcgc cgacgacggt ggtcaggtgc tgtgcgcgac
     1021 gcactcaccg gtgctcgcgg cgctgccggg cgcgcagatc ctggaggtcg gtgaatgggg
     1081 cctgcggccg gtcgcctggg acgacctcga gctcgtccgg cactggcgct cgttcctcag
     1141 cggtcccgag cgctacctgc ggcacctgct ggactgaggt gcctcaggcg acctgcgcca
     1201 ggacccgcgt caacttgtgc ggattgcaga cctgtcggat gccgcggatg cggccctgct
     1261 catcgaactc gagcgagagc acatcggtgc ggccggagtg cgtgagcgcg aacgccggct
     1321 cgccgttgac gtcgatcgac tcgagggtga acggatggac cggatcgaac accgccgcct
     1381 tcgcctgcac gccgaggtag aagcgcgcga tcttctcggc gccgaagatc gggttgcggg
     1441 tcgcggagac gacgcccccg ccatcggtcc agaggatcgt ctcgtcggtg agcaggccga
     1501 gcagagcacc gagatccccg ccctgcacgg catcgaccag ggtggacagc gcctggggat
     1561 cggtggcacg cggcggtgac gcatcctcgt cgagccgcag ccggcgctca ccgcgcgaga
     1621 tcagctgccg cacagccgcc ggcgacttct cgaggatctc ggcgatctcg gtggccggca
     1681 tcccgaaggc gcggtgcagc accacggccg cgcgcgcatc gggggtgagc tgctcggcca
     1741 gatggagcag ggcgagcgag agcatctctc gatcggcgac cgcatcctcc gggaggcgag
     1801 aggtctccac gggctcgggc aaccagtgcc cggtgtagtc ctcgcgcacg gcggccagtg
     1861 atcgcacccg gtcgatggag cggcgcacgc aggtggtcgt gagccatgcc ggccaggagc
     1921 gcacgctgcc ggcatccgcc tgctccgtcc gcagggcgtc gatcgccacc tccgagacga
     1981 cgtcctcggc atggccgaag tcacccagca tccggtaggc gatgccgatc agcctgccgc
     2041 gctccgcctc ccaggcgcgc gcggcaggtg tcgcgacggt ggtcatggaa tcaggctacg
     2101 accccgtcag acgatcggat gcctgtcgtc gaccggcggc tgcatctcgg tcgcgatcgc
     2161 gatgcggttc cacacgttga tcgtcgcgat cgccatcacg atggctccca gttcgctctc
     2221 gtcgaagtgc tcggcggcgg cgttccagat ctcatcggtc acggtgtccg gaccgagctt
     2281 ggtgatcgca tccgtcagcg cgagtgcggc gagctccttc ggctcgaaca ggttcttcgc
     2341 atgctgccag gcgccgacgg cgtgcagggt gcgctgcggg atgccgcgct tggcgccgtc
     2401 ggtcgcgtgc atgtcgacgc agaacccgca gccgttcagg atcgatgccc gcagcttgat
     2461 cagctcgtac agccgcctct ccaccgactt gctggcgtac gcctccatcc cgagcaccgc
     2521 ggcgtagccg agcttgttcg tgcgtgccat gtcgatcctg gtcatgatgt tccttccgtg
     2581 gcgtccgatt gccttcacct gtatgtcgga tgccgagcgg gaagtgtgac atccgtcatc
     2641 cgtctccggt tcatcgcccg tcctgcgaca atggaggacc gagcgatcga cccgaggagt
     2701 cctcttgcgg tacatctcca cccgcggcgg catgacgccg cagccgttca gtgagacgct
     2761 gctcgagggc ctcgcgcccg acggcggtct ggccgttccc gagcagctgc cgcaggtcga
     2821 ggccgagacg ctggagcgct ggcgcgccct cacctacccg cagctggccg cggaggtgct
     2881 cggcctgttc gccaccgaca tcccgcgcga ggacctcgcg cgcatgacgg ccgacgccta
     2941 cgccgacttc cccgacgcgg tcgtgccgct gcgctccatc ggcgacggca tcacgctggt
     3001 cgggctctcc gaggggccga ccctggcgtt caaggacatg gccatgcagt tccttggcca
     3061 ggcgctggac tacgtgctgg agcgcaagga ccaggtcctg aacatcgtcg gggccacctc
     3121 gggtgacacc ggctcggcgg ccgagcacgc gctgcgcggc aaggaccggg tctccgtgtt
     3181 catgctctcg ccgcagggca ggatgagctc gttccagcgt gcgcagatgt actcgctcga
     3241 cgacgcgaac gtgcacaaca tcgccgtcga gggcgtgttc gacgactgcc agaacctggt
     3301 caagaagctc gcgggagacc tcgagttcaa gcgcgcgcag cacctcggcg cggtcaattc
     3361 gatcaacctg ggtcgcatcg ccgcgcaggt cgtctactac ttctgggcct ggctgcgggt
     3421 cacggatgcc gtggaaccgg agttccggtc cggcttcgag gtctccttca cggtgccgtc
     3481 gggcaacttc ggcaacatcc tctcggggtt cttcgccaag ggcatgggcg tgccgatccg
     3541 caggctcgtg ctcgccgcca acgagaacaa cgtgctcgac gagttcttcc gcaccggcgt
     3601 gtaccggccg cggtcggccg ccgacaccca cgccacctcc agcccttcga tggacatctc
     3661 caaggcgtcc aacctggagc gcttcatctt cgagctggtc ggacaggacc cttcgcgcgt
     3721 cgccgacttc ttccgctcga tcgacgagca ggggttcgtc gacctctcgg ccgagcagcc
     3781 gcgattcgaa gccgagttcg gcatcgtcag cggcacctcc acgcacgccg accggctcgc
     3841 cacgatccgt tcggtgcacg aggcatccgg cgagatcatc gacccgcaca cggccgacgg
     3901 cgtcaaggtg gcccgcgagt acgtcgagac ggacgttccg atgctggtgc tcgagaccgc
     3961 caagccgcag aagttcgccg agaccatcac cgaggcgatc ggcgtcgagc tgaagttctc
     4021 tgccgagctg cagcggatgc tggccgcccc tcagcacgtc accgagatgg ctgacgacga
     4081 gcaggcgctg cgcgccttcg tcgcggcgaa cgccctgcac tgagccggcg gggtctgtcc
     4141 cctcccgctc cgtcccggtc cgatccgaac cgcgcgatga tccacgctgc gccgtaccgg
     4201 cccgagtccg ggacggcgag cacggccgcg ccgagcagca tcatcagcgg atattgcgcg
     4261 atgagctggt ccgaccccca atagagcgcc agcgtggcga tggagccgag ggcgaccagc
     4321 cgggtgagca caccggcgat cagggccagt ccgagcagaa cctcgagtgc cgggatggct
     4381 aggccgaagg cgtccgggag cgcctgcatc gtggcgcgta caggggtgaa ccaccagggt
     4441 gtgcggggat tcgacgcggt gccgtccgcc accagcagga tgtcggccgc accgaagccc
     4501 gcccggtact tctccagccc ctcgagaacc cacagcacgc cgacggccac cctgaccgcg
     4561 ctgccgagca gcggcgcggt cacggccggc acggcgttca tgagagcttc gcggcgccga
     4621 acaccgcctt ggccttgtcg cagtggatca cgacgtacga gtacatggac gtgtcgaccc
     4681 ccgcgagcat gaaggtctgc gactgcttgt cgaaggcgac cgcgtcgatc tccttgcccg
     4741 ctccgacggc tgcctcgtcg gtgccgctcg tgaggtagac gtgcaggtcc gggccctcat
     4801 ccgatgagaa gccggagagc gtgagcgtgc cgtcctcgac gctcgccatg ccggagacgg
     4861 tcttgccgtt cagaccctcg aaagagcccg tcatgggatc ggccatgggc gtcgagccgg
     4921 agtcgctgcc ctgactcatc ggggccttgc tcggcatgtc ggaacccggc gtcgagctgc
     4981 agcccgcgag cgtcagtccg agggcgatca cggcgacggg gatcgcggcg gtgaaggtgt
     5041 tcttgcgcat gatgtctcct ggttctgccg acgaggtgtc gcgtcggccc gacttcgacg
     5101 gtagggagac cgcggatgcc cggggcggga gctttcagaa tcgtaagaac ctgccgccgc
     5161 cccgctcccg cacggttagt gtcgagcgca tggacagcag ccgagtcgtg gtcgtcgagg
     5221 acgatccggt ggtccgcggc gcggttgggg actatctgcg cgctcgcggc taccgcctcg
     5281 acgcattcgc cgacggggtc gcggcgcgcg aggcgatcgg tgccgagatc ccagacctcg
     5341 tgatcgtcga ccggatgctg cccggcatca gcggagatga gctgtgccgt gcgatccgcg
     5401 agggcagcga tgtgccgatc atcatgctga ccgccctcgg agaggtggag cagcgaatcg
     5461 aggggttcga ggccggcgcc gacgactacc tcgccaagcc cttcgcactg cgggagctcg
     5521 agctgcgcgt ggatgcgctg ctgcgcaggt cgatgcgcgc tccggcaccg tcggcgttca
     5581 ccgcgggcga cttcgagatc gatgcggcga ggcggaaggc ccgcgtgcgc ggcactgaga
     5641 tcgctctgac cgggcgcgag tacgagctgc tggtccacct cgtccgcaac tcggaccgca
     5701 cgatctcacg ggacgagatc ctgcgcgagg tctggggctg gagcttcggc gagccctcca
     5761 ccgtcaccgt gcacgtgcgg cgtctgcggg agaagatcga ggaggatcca cgtgaacccc
     5821 ggtacctgca caccgtgtgg ggcgcggggt accggttctc ggcgaaggcg acggagctct
     5881 gatggccgag atgatcccgc tcggcgtcgt cgacgtcctg ctgatcgcgg gcaccgctct
     5941 ggcgggcacg gtcgtcgtcg ccgccatcgc cctgatcctg ctgcggtggg cccgcggcgg
     6001 ttcgatgctc gtgcagatgc tcgtgctcgc ctccgtcgcc gcggtgtcga tggcggccgc
     6061 ggtgctgctc atctcggtcg agatgtacat ctcgccgcat gacctcagcg tgctggtgtg
     6121 ggtgatcggc acgtcgctgc tgttcagcct gctcgcggcc tggctcgtcg cgcggctgac
     6181 gcgcgcccgc ctggtccgga tcagcgaggc agtgcgccgg gtgggcgagg gcgacgtggt
     6241 cgcggccgag ccgcacgggc tccgcgagtt cgacgatctc tccgtggagc tcgccgacgt
     6301 ctcggagcgg ctgcacgccg cgcggcagga gctggagcag ctggactccg cccgccgccg
     6361 gttcttcgcg tggatctcgc atgatctgcg tacacctctc acagccgtgc gggcgctgtc
     6421 cgaggccatc gaggacggcg cttccgccca gccggagcgc tacgcgggcg aggtgcgtgc
     6481 gcagatcgac acgatgagcc ggatggtcga cgacctgttc gagctctcga agctcacctc
     6541 gggtgcggtc cggctgcgca cgcagcaggt ggagctgctc gacgtcgtct cggacgcggt
     6601 cgcggatgta cggctggcgg ccgagcaacg cggcgtcacg atcgtggagc ggggcgtggg
     6661 tggtcacgtg ctgtgggccg acccgcatca gctgggcagg gtggtggtga acctgctcac
     6721 caatgcggtc cggcacgcgc cccgcgacag cgagatcctc gtcacggcga cagagctgga
     6781 cgagcagcgc ctcgtgctcg gcatcctgga ccacggttcg ggcgtcgcga cgtcggatct
     6841 ggatcgcatg ttcgaggtcg gctggcgtga ggacgccgcc cgtcccacga ccgccaacga
     6901 ccccgtcgca tccggggcgg ggctgggact cgcgatcgcc cgcggcctgg cgcgcgcgca
     6961 cggcggagac gtgtacgccg agcacaccgg tgagggattc cggatgaacg tcctcatgcc
     7021 tgtcggcgac gagagcacgc agcgctgagc gcggacgcac cgttcgacgc cgggcagcgc
     7081 agaccggatg ctctctctcg ctccgagcct gccaggggtg aacagtgcat cacgcacatc
     7141 cagtcgagat tctcgtgcag aacgcacata tcgcgctggg tgaggcgtgg ctactgttgc
     7201 cacaaccctc atccctcctc tggaaggcac cctcatggca cgcatggcgc acgccgacga
     7261 cttcgcactc tcgcgggtca ctcccgaggc gcgcaaaccc tggttcggga tcgcactgca
     7321 gcgattcggg caggtctccg cgctctcgca gttcctgctc ggcgcgaccc tcggcttcag
     7381 catgacattc ggtgaggccg tcctcgcctt cctcttcggc tcgatcatcc tcgaggtgat
     7441 catgtgcgtg gtcggattca tcggccagcg cgaggggctg aacacggccc tgctcgcacg
     7501 gtggaccggt ttcggcgaga tcggagcctc gctggtcggg ctcgcgatcg gcatcagcct
     7561 gatcggctgg ttcggcatcc agtccgcgat ctcggcgcag tcgctcgacg cgctcatgcc
     7621 cggcgccctt cccatctggg cgtggagcct gatcttcggc ctcgcggtga ccgccatcgt
     7681 cgcgttcggc ttcatcggca tgcagtggct cgccaacgtc accgtgccgc tgttcctgat
     7741 cctcgtcgga tggtcggtca tcggcgagct cagccgccac gacatcggcg agctgctgac
     7801 cagccccgcc cccgggccga cgatgagcgt ctgggcgggt accggcatcg tcgccggcgg
     7861 cctgatcgtc ggcgcgatca tcaccggcga catgacccgg ttcaaccgct ctcgcgccga
     7921 cgtggtgaag cagaccgtcg tgggcgtctc gctcggcgag ttcgtcatcg gcctcgcggg
     7981 cgtgctgctg gcccacgccg ccggcaccgg cgacatcgtc gcgatcgtga cctcgtcggt
     8041 cggtttcatc ggcctgttca tcgtgatcac cggcacgctg aagatcaacg actggaacct
     8101 gtactcctcc acgctcggcc tggtgaactt catctccacg gcgttcggcc gcaacctgca
     8161 ccgcgtcacg accacggtcg tgctcggcgt cgtcggctcc gtgctcgccg cggtcggcat
     8221 cctcggccag ttcacctcgt tcctgatcgt gctgagcgtg gccttcccgc cgatcgccgg
     8281 catcatggtc gccgagtact tcatcgtgcg ccgctggcgc ggcgagctcg acgccacccg
     8341 cgactcgggg acgctccccg cgaccgcgcc gcgactcgtg ccggcgaccc tcatcgtgtg
     8401 gctggtgtcc tccctggtcg gctacttcgt cacctggggc atcccgtcac tgctgagcct
     8461 cttcctctcg atggtgctct acgtcatcgc cggcaagctc ggcctggtgc gcggcgtcgg
     8521 cgtcgccacc acgcggcaga cctccgcatc cgattcgcag cccgtcggcg cctgagcgcc
     8581 cggcctccac acagaaagcg agaaccatgc acatcggcat cgacgtcggc ggcaccaaca
     8641 ccgacgcagt cctgatggac gccacccgca cgctggcggg ggtcaagcac tccaccacct
     8701 ccgacgtgac gagcggcatc gttcaggcca tcgaggacct gcgcgccaac caccccttcg
     8761 agggcgacga catcgatgcg gtgatgatcg gcaccacgca cttcatcaac gcgctcgtgc
     8821 aggccagccg cctcgccccg acggcggcgc tccggctggg gctgcccgcc accagggcgc
     8881 tcccgcccct ggtcgactgg ccggaggtgc tcgtcgaggc cacgcatgcc cgcagctacc
     8941 tggcgcacgg gggctacgag ttcgacgggc ggccgatctc gccgatcgat cccgaggagc
     9001 tgcgcgcgca cgccgaggac atgaaggcga acggcatccg ctccgtcgcc atctcgtcgg
     9061 tgttcagccc ggtgaaccac gacctcgaat cgcaggccgc cgagatcatc ggcgaggtgc
     9121 tgggcgacga cgtcgccatc tcgctgtcgc acgagatcgg ccggatcggt ctgctcgagc
     9181 gcgagaacgc gacgatcatc aacgccgccc tgcgcgagct cgcctcggag atcgtcgacg
     9241 ggctcacctc cgccgtgcgc gcacagggca tcgaggcgcc gatcttcctc agccagaacg
     9301 acggcacgct gatggacgag gagtacgtgc gccgctaccc ggtggccacc ttcgcctccg
     9361 gtccgaccaa ctcgatgcgc ggcgcggcga ccaccagcgg tctcgacgac tgcgccgtca
     9421 tcgacgtggg cggcaccacc gccgacatcg ggctgctcat caacggattc ccgcgcgaga
     9481 cggcgaacga ggtgaaggtc gccggcatcc gcaccaactt ccgcatgccc gacgtgctgt
     9541 cgctcggtat cggcggcggc agcatcgtcg acgaggagac cgccgaggtc ggtcccgcat
     9601 ccgtcgggta tcggctcgcc accgaggcgc tcgtgttcgg cggcagcacc ctgacggcca
     9661 ccgacatcgc cgtggccgcc ggccgcgccg acgtcgggga cgcctcaaag gtcgcccacc
     9721 tcgacccggc gttcgtcgcc cgtgtgctcg agcgcatcgc cgaccgggtc tccgaggccg
     9781 tcgaccggat gcgcacctct cctgagccga tcgccgtcgt cgccgtcggc ggcggatcga
     9841 tcctgctgcc cgatgagctg cccatgttcg gcaccgtgca ccgaccggag aactacgccg
     9901 tggccaacgc catcggcgcc tcgatcgccc aggtcggcgg cgagatcgac aaggtgtacg
     9961 ccatcgagcc cggtcgtcgt gacgagacga tcgctgccgt gcgcgccgaa gccgtcgaca
    10021 aggccatcgc tgcaggcgcc aagccgtcca cggtgtcggt catcgacttc gacgaggttc
    10081 ccatcccgta tcttcccggc aacgccacgc gcatccgcgc gaaggccgtc ggcgacctcg
    10141 acatgggggc gtgacatgac ctggacgatc accgccgagc acatcgacgg actcgcccgc
    10201 ggcgcggcgg tgctcggcac cggcggcggc ggggacccct acatcggagc cctgctggct
    10261 cgtcaggctc tcgccggcgg cgacgtcacc gtcgtcggcc tggatgaggt gcccgatgac
    10321 gcggtcgtgc tcttcgtcgc catgatgggc gcaccgaccg tgatggtcga gaagctgccc
    10381 agcctggacg aggtcgtcga accggtccgc gctctcggcg tgcatctcgg ccgccccatc
    10441 acccacatcg catgcgccga ggtcgggggc gtgaactcca ccatcccgat cgccgcggcc
    10501 gcagctctcg gcctgcccct gatcgacgcc gacgggatgg gtcgcgcatt ccccgagctg
    10561 cagatggtgc tccccacgtt gtacggcgtt accgcctcgc ccctggcgtt cagcgatgag
    10621 aagggcaaca ccggcgtgct gcggaccgtc gacaacgcct ggaccgagcg catcgcccgc
    10681 gtcgcgtgcg tggagatggg atgctcggtg atgatctcgg gcttctcgat gtctggcacg
    10741 caggcgcgcg actcgctcgt ctccggatcc ctgtcgcgct gcgtcgagat cggacaggcc
    10801 atcgtcggag cacgcgagga gaaggccgac ccggtggcgg ccgccgtggc gttgctgaac
    10861 gggcgcgagc tgttcgacgg caaagtcgtc gatgtgcagc gcgccaccac caccggattc
    10921 gcgcgcggac gcgcgcggat cgacggtgat ggcgactcgt cgatcacgct gcagttccag
    10981 aacgagcacc tcgtggccga gaaggacggg caggtgctcg ccacgacgcc cgacctgatc
    11041 atggtgctcg agggcgagtc cggcgagccg atcaccaccg aggcgctgcg ctacggccag
    11101 cgcgtgcgcg tgatcgccgc cccggccgac gaacgctggc actccgccga ggcgctcgcg
    11161 atggtgggcc cgggctactt cggctacgac atgcccgcgc atcgcttcga tggcacgatc
    11221 tccgtcgggc atggcgccga agcggcggga gaagcggcgg gagaagcggc gtgagctggg
    11281 agctgactgc cgccgatctg cccgacctcg ctcggggcgc caccctgctc ggcaccgggg
    11341 gaggagggga tccgtacatc ggcaagatgc tggtcgagcg cgtgctcggc gatcgctcga
    11401 tcaccatcct cgatccggat gagctcgccg acgacctctt cgtcatcccc accgcgcaga
    11461 tgggcgcgcc gaccgtgatg gtcgagaaga tccccgccgg aaccgagccg acgctggcac
    11521 tgcgcacgct ggagaagcac ctcggccgca cggccgacgc gaccatgccg atcgagtgcg
    11581 gcgggatcaa ctcgatgatc ccgctggtcg tcgcagccga gacgggtctg cccgtggtgg
    11641 atgccgacgg catgggccgc gcgttccccg agctgtcgat ggagaccttc gccgtgtacg
    11701 gcgtgcacgg ctctcccctc gccctcgcgg gcgagcgcgg cgagcaggtc gtgatcgaca
    11761 ccggtgacga cgaccgccag atggaatggc tcgcccgcgg catcacgatc aggctcggag
    11821 gcgtgggcca catcgccgag tacgcgatga ccggcgccga tgtgcgccgc accgcggtgc
    11881 cgcggacgat ctcgatggcc ctcgcgctcg gccgcgccat ccgcctcgcc cgcgaggagc
    11941 atcgctcgcc gttcgaggcg atcggcgcga ccctcgcctc gaccctgtac ccgcacctgc
    12001 gcgagctgca cgtcgggaag gtcgtcgacg tcgagcgccg cacgaccgag ggcttcgcga
    12061 agggcagagc ggtcatcgca ccgctcggcg atgacggcga gacgctcgag atctcgttcc
    12121 agaacgagaa cctcatcgcc cgtcgcggcg ggcgcctcgt ggcgatcgtg cccgacctca
    12181 tctgcgtcgt ggaccacgag accgcggagc cgatcaccac ggaagggctg cgctacggcc
    12241 agcgagtgcg ggtgctgggc atctcgactc cggcgatgat gcgcaccccc gaggcgctcg
    12301 tcgccttcgg accgtccgcg ttcggtctgc aggaggactt cgtcgccgtc gaggagctgg
    12361 ccgggcccta acctggatgc atggtgagcc ggctcgggca agacgatctg accgcgctcg
    12421 cgcgcggtta cgcgctgctc ggctcgggcg gcggcggacc gaccaccatg ctggagctca
    12481 tgctcggcgg atcggcggca tggccgatcg atgtggtcga tccttccgct ctcgatcccg
    12541 cgacaccgtg cctgggcgtc gcgttcgtcg gctccaccat gctcctgagc gagcggatgc
    12601 ccgggatcga ccccttcggg ccgctgatcg ccgccgtcga gcggtggctc ggacacgagg
    12661 tgcccgccgt gtgctcgctg gagggcggcg gcatgaacgg cctcgccccg ctgacgctcg
    12721 ccggcacgca catcgtggtc gatgcggact gcaccggccg cgccgttccc ggtctcgacc
    12781 agatgtcgct gttcgtggac cgggtgccgg gcatcgtggt cgcctgcgac accggtgccg
    12841 gcggggtggc gctcgtcgag gcggcgcgtg cgatcgacgt cgagcgggtg gtgcgcgccg
    12901 cgatcatcca ggcgggcggc gccggttgcg cggtcttcgc gggattcacg gtcggcgacc
    12961 tccgcgagca cgcgatctcg ggccacctgg cgcacgctct cgacctcggc cgtgccttcg
    13021 tggcagcatc cggacgtccc ctcgccgagc tggcggacgc gctcggcgcg cgactggtcg
    13081 ccgaggggcg catcctgtcg gtctcggccc tcgccgatga cccgcacgtc agcgccgtgg
    13141 agatcgcagg cgccggcggc acggtgcacc gggtgatcac gcgctccgag acgctcgcgg
    13201 tggtgagcga cggacaggtc gtcgccgcgg cgccgacggt catcgtcgtg gtcgatgcca
    13261 tctcacgcga ggtgctggag gtcactgaac tgtccctcgc ccgaaatgtg gccgtgttcg
    13321 ccatcccggc gccggcgtgg tggatcgcct ctcctggccg cgtcgagcat gtgcgcccct
    13381 cggcgtacgg cctcgccgag ctggacgtgg catgagcgcc gggctgcgcc tcgcggcgct
    13441 gctc
//
LOCUS       DATMSX010000345         5663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_128622,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000345 DATMSX010000000
VERSION     DATMSX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128622"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..>5663)
                     /gene="pulA"
                     /locus_tag="VN041_10805"
     CDS             complement(51..>5663)
                     /gene="pulA"
                     /locus_tag="VN041_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HWU29563.1"
                     /translation="TVALVGDLQSELGCAADWAPDCTQTELAPTGAAGIYSAEFTVPA
                     GSFEYKVALNDTWDEAYGLDGGGDNIPLTVAGPATLRFTFDDNLKRVGVQATGLRQGY
                     TADDDALIAKPVRQPGSEEQFYFVMTDRFANGDTANDTGGLTGDRLTTGFDPTDKGFY
                     QGGDLKGLRDRLDYIEGLGTTAIWLTPSFANKPVQGTGANASAGYHGYWVTDFTRIDP
                     HLGTNAELESLIADAHARGIKVYFDIITNHTADVVDYAQKQYSYIDQATSPYKDADGK
                     AFDPADYAGTDGFPTLDASTSFPYTPVIADADKDAKTPAWLNDPTLYHNRGNSTWSGE
                     SVTYGDFDGLDDLMTENPRVVDGFVKVYQDWVDLGIDGFRIDTVKHVNFEFWKTWSTR
                     VLDYAHAAGKKDFFMFGEVYDADPVKLAPYVRDTDMNSVLDFTFQSSAVSYASGNSAK
                     GLQSLFAGDDRYTTPDSSATALPTFLGNHDMGRVGSFLQRTDAPLQRTELANELMFLT
                     RGQPVVYYGDEQGFAGPGGDKDARQTLFATQVDEYANQKLITGEQAGSVDRYGTDAPL
                     YRQIGALSKLRAGIPALRDGAQIERYAASGAGVYAFSRVDAADKVEYLVAVNNATSAQ
                     TVDLTTLTSDASYAVQYGDGQAITTDAGARTSVTVPALSAVVWKADAPVSAPGEAAPV
                     TISAPAPGGAVSGQQPVQAAIADQWAQTSLSWRVVGSDEWHALGTAEDTDPRVFHDIR
                     GLKKGTLLEYRAVTTDAAGHHAAASTYASVGNAVSLGAGEEQPESPIDMVTVPGSLNS
                     EMGCAGDWDPACEKAKLTLRSDGVWAGTFDLPAGDYEYKAAIDGSWTVNYGANGVADG
                     PNITISHKGGPISFYFDPRTNIVQSSAEGPIVTLPGSLQDELGCAGDWMPDCLATLMA
                     DGDRDGVYEFSTSKLPTGAYELKVAHGLSWAENYGADGARDGANISFSATEGKVTSFR
                     YTLATHVLEVASDDAPLPGTGEQRAQWIDRDTIAWPATLGQTDRAAYRLHSASDASLT
                     VTDGQVTGGDAVDLTVVDGGLTDAQKKRFPALAGYLALKVPADADAPALLRTQLAVAQ
                     SDDAGALTAFTGVQLPGVLDDLYAAKAADAELGAGFSGVNPTFRLWAPTAQSATLLTW
                     DAKAGQDAEPTRHKATFDAASGVWTVKAKKALKGVEYLWEVAVYAPTTGEIETNRVTD
                     PYSVALTLNSERSVAVSLDDKAFQPKAWKTTKSPVIDKPADRAIYELHIRDFSIGDDT
                     VPEAERGTYLAFTRDSAGTKQLRQLADAGMNTVHLLPSFDIATIQEERRSQAVPDCDL
                     TSYGPADTAQQACVAAVADTDGFNWGYDPYHYSTPEGSYAVNPEGGARIAEFRSMVGA
                     LHGMGLQVVLDQVFNHAAASGQAERSVLDRLVPGYYHRLNAAGAVETSTCCQNVATEH
                     LMAQKLMVDSLVTWARGYRVDGFRFDLMGHHSVANMMAARKALDALTLEDDGVDGKRI
                     YLYGEGWNFGEVANNALFPQAVQGQLGGTGIGTFNDRLRDAVHGGSPVDGGSTFMQGF
                     GTGLATDPNGDPINGSAAQQLATLGHDTDLVKLGLAGNLRDFAFTTSDGRTTAGKDID
                     YNGQRAGYADQPDEVINYVDAHDNETLYDLSVLKLPVSTSMADRVRMNTVELATVTLS
                     QSPSFWHAGTELLRSKSLDRNSYNSGDWFNRIDWTGKQSTFGSGLPTAADNQGKWDIM
                     APLLQNPALKPKASDMAAAEASALDLLRVRSEVGLLRLGSADLIRQKVSFPNGGADAA
                     PGVILMQIDDLVGKDVDPALEGALVVFNASPNPVTQTVAGLAGRDLVLTPAQAKGSDA
                     VVKTTTWDAATGTVTIPARTVAVLVDVQ"
BASE COUNT          742 a         1850 c         2096 g          975 t
ORIGIN      
        1 ccctcacggg gccggcgctt tcggttgcgg cgggggctcc ccgctcggga ctactgcacg
       61 tccaccagca cggcgaccgt gcgggcgggg atcgtgaccg tgccggtcgc ggcatcccag
      121 gtggtggtct tcaccacggc atccgagccc ttcgcctgcg cgggcgtcag cacgagatcg
      181 cgaccggcca ggccggcgac ggtctgcgtg accgggttcg gcgaggcgtt gaacacgacc
      241 agggcgccct cgagcgccgg gtcgacgtcc ttcccgacga gatcgtcgat ctgcatcagg
      301 atcacgccgg gcgcggcatc cgccccgccg ttcgggaagc tgaccttctg ccggatcaga
      361 tccgcggagc ccagccggag caggcccacc tcgctgcgca cgcgcagcag atcgagagcg
      421 gatgcctcgg cagcggccat gtcggatgcc ttcggcttga gcgcggggtt ctgcagcagc
      481 ggggccatga tgtcccactt gccctggttg tcggccgccg tcggcagccc agagccgaac
      541 gtggactgct ttccggtcca gtcgatgcgg ttgaaccagt ctccggagtt gtagctgttg
      601 cgatccagcg acttcgagcg cagcagctcg gtgccggcgt gccagaacga cggcgactgc
      661 gacagggtga ccgtcgccag ctcgacggtg ttcatgcgca cgcggtcggc catcgaggtg
      721 ctgaccggca gcttgagcac cgacaggtcg tacagggtct cgttgtcgtg cgcgtcgacg
      781 tagttgatca cttcgtcggg ctggtcggcg tagccggcgc gctgcccgtt gtagtcgatg
      841 tccttgcccg cggtggtcct gccgtcgctc gtcgtgaacg cgaagtcgcg caggttgccg
      901 gcgaggccca gcttcaccag gtcggtgtcg tggccgaggg tcgcgagctg ctgcgccgcg
      961 cttccgttga tggggtcgcc gttcgggtcg gtcgcgagtc ccgtgccgaa gccctgcatg
     1021 aacgtcgagc cgccgtcgac cgggctgccg ccgtgcacgg cgtcgcgcag ccggtcgttg
     1081 aaggtgccga tgccggtgcc gccgagctga ccctgcacgg cctgcgggaa cagggcgttg
     1141 ttcgcgacct cgccgaagtt ccagccctcg ccgtacaggt agatgcgctt gccgtcgacg
     1201 ccgtcgtcct cgagcgtgag cgcgtcgagg gccttgcgag cggccatcat gttggcgacc
     1261 gagtgatggc ccatgaggtc gaaccggaag ccgtcgacgc ggtagccgcg ggcccacgtc
     1321 acgagcgagt cgaccatgag cttctgcgcc atgaggtgct cggtcgccac gttctggcag
     1381 caggtcgagg tctccaccgc gcccgccgcg ttgaggcggt ggtagtagcc ggggacgagc
     1441 ctgtccagca cgctgcgctc ggcctggccg gatgccgcgg cgtggttgaa cacctggtcg
     1501 agcaccacct gcagacccat gccgtgcagg gcgcccacca tggagcggaa ctcggcgatg
     1561 cgcgcaccgc cctccgggtt cacggcgtac gagccctccg gcgtggagta gtggtacggg
     1621 tcgtatcccc agttgaagcc gtccgtgtcg gcgactgccg cgacgcacgc ctgctgcgcg
     1681 gtgtcggccg ggccgtacga ggtcaggtcg cagtccggca ccgcctggga cctgcgctcc
     1741 tcctggatcg tcgcgatgtc gaaggagggc agcaggtgca cggtgttcat gccggcgtca
     1801 gccagctgcc gcagctgctt cgtgccggca ctgtcgcggg tgaacgccag gtaggtgccg
     1861 cgctcggcct cgggaacggt gtcgtcgccg atcgagaagt cccggatgtg cagctcgtag
     1921 atcgcacggt cggcgggctt gtcgatcaca gggctcttcg tcgtcttcca cgccttcggc
     1981 tggaacgcct tgtcgtcgag gctgacggcg accgagcgct cggagttcag cgtgagcgcg
     2041 accgagtagg ggtcggtgac ccgattggtc tcgatctctc cggtggtggg cgcgtacacc
     2101 gcgacctccc acaggtactc gacgcccttg agcgccttct tggccttcac ggtccagact
     2161 ccggatgctg cgtcgaaggt cgccttgtgg cgggtgggct cggcgtcctg gccggccttc
     2221 gcgtcccacg tgagcagggt ggcgctctgc gcggtgggcg cccagagccg gaacgtcggg
     2281 ttgacgccgc tgaagccggc gccgagttcg gcatccgccg ccttcgccgc gtacaggtcg
     2341 tccagcacgc cgggcagctg cacgccggtg aaggcggtga gcgcacctgc gtcgtcgctc
     2401 tgcgcgacgg ccagctgcgt gcgcagcagc gccggcgcat cggcatccgc cggcaccttc
     2461 agagccaggt agccggcgag cgcggggaag cgcttcttct gggcatccgt caggccgccg
     2521 tcgacgacgg tgaggtcgac cgcatcgccg ccggtgacct gaccgtcggt cacggtcagc
     2581 gaggcgtcgg atgccgagtg cagccggtac gcggccctgt cggtctgccc gagcgtggcc
     2641 ggccacgcga tggtgtcgcg gtcgatccac tgggcgcgct gctctcccgt gccgggcagc
     2701 ggggcgtcgt cactcgcgac ctcgagcacg tgcgtggcga gggtgtagcg gaaggaggtg
     2761 accttgccct cggtggcgct gaacgagatg ttcgcgccgt cgcgggcgcc gtcggcgccg
     2821 tagttctcgg cccagctcag gccgtgcgcg accttgagct cgtaggcgcc ggtgggcagc
     2881 ttcgaggtgg agaactcgta gacgccgtcg cggtcgccgt cggccatcag ggtggccagg
     2941 cagtccggca tccagtcacc ggcgcagccg agctcgtcct gcagggagcc ggggagcgtc
     3001 acgatcgggc cctcggccga ggactgcacg atgttcgtgc gcggatcgaa gtagaacgag
     3061 atcggtccgc ccttgtggct gatggtgatg ttcggtccgt cggcgacgcc gttcgcgccg
     3121 tagttgaccg tccagcttcc gtcgatcgcg gccttgtact cgtagtcgcc ggcgggcagg
     3181 tcgaaagtgc ccgcccacac gccgtcgctg cgcagtgtga gcttcgcctt ctcgcaggcc
     3241 gggtcccagt cgccggcgca gcccatctcg gagttcaggc tgccgggcac ggtgaccatg
     3301 tcgatgggcg attccggctg ctcctccccc gcgcccagcg agacggcatt gccgacggag
     3361 gcgtaggtgg acgcggcggc gtggtggccg gcggcatccg tggtcaccgc gcggtactcg
     3421 agcagggtgc ccttcttcag cccgcggatg tcgtggaaga cgcgcgggtc ggtgtcctcg
     3481 gcggtgccga gtgcgtgcca ctcgtcggag ccgacgaccc gccaggagag gctggtctgc
     3541 gcccactggt ctgcgatggc ggcctgcacc ggctgctgac cgctgacggc gccgccgggt
     3601 gccggggcgc tgatcgtgac cggtgcggcc tcgccgggag cggagaccgg ggcatccgcc
     3661 ttccacacga cggccgacag ggccggcacg gtgacggagg tcctggcacc ggcatccgtg
     3721 gtgatcgcct gcccatcgcc gtactgcacg gcgtacgacg cgtcggaggt gagggtggtg
     3781 aggtcgacgg tctgcgcgct ggtggcattg ttcaccgcga ccaggtactc caccttgtcg
     3841 gcggcgtcga cacgggagaa ggcgtagacg ccggccccgg atgccgcgta gcgctcgatc
     3901 tgggcgccgt cgcgcagcgc cgggatgccg gcgcgcagct tcgacagcgc cccgatctgc
     3961 cggtacagcg gggcgtcggt gccgtaacgg tcgacggagc ctgcctgctc gcccgtgatg
     4021 agcttctggt tcgcgtactc atccacctgc gtcgcgaaca gcgtctggcg tgcgtccttg
     4081 tcgccgccgg ggcccgcgaa gccctgctcg tcgccgtagt agacgaccgg ctgcccgcgg
     4141 gtgaggaaca tcagctcgtt cgccaactcg gtgcgctgca gcggggcatc cgtccgctgg
     4201 aggaacgatc cgacccggcc catgtcgtgg ttgccgagga acgtcggcaa cgcggtggcg
     4261 ctggagtcgg gcgtcgtgta ccggtcatcg ccggcgaaca ggctctgcag gcccttggcg
     4321 gagttgccgg atgcgtagct gacggcggag gactggaagg tgaagtccag caccgagttc
     4381 atgtcggtgt cgcggacgta gggggcgagc ttgaccgggt cggcgtcgta cacctccccg
     4441 aacatgaaga agtccttctt gcccgccgcg tgggcgtagt cgagcacccg ggtcgaccag
     4501 gtcttccaga actcgaagtt cacgtgcttg acggtgtcga tgcggaagcc gtcgatgccg
     4561 aggtcgaccc agtcctggta aaccttcacg aacccgtcca ccacccgcgg gttctcggtc
     4621 atcaggtcgt cgagtccgtc gaagtcgccg taggtgaccg actcgcccga ccaggtcgag
     4681 ttgccccggt tgtggtacag cgtggggtcg ttcagccagg cgggtgtctt ggcgtccttg
     4741 tcggcgtcgg cgatgaccgg ggtgtacggg aagctcgtgg acgcgtcgag cgtggggaag
     4801 ccgtcggtgc ctgcgtagtc ggcggggtcg aacgccttgc cgtctgcgtc cttgtacggg
     4861 ctggtggcct ggtcgatgta ggagtactgc ttctgcgcgt agtcgacgac gtcggcggtg
     4921 tggttggtga tgatgtcgaa gtacaccttg atgccgcggg cgtgcgcgtc ggcgatcaac
     4981 gactcgagct cggcgttggt gcccagatgc ggatcgatgc gggtgaagtc ggtcacccag
     5041 tagccgtggt aaccggcgct ggcgttggcg ccggtgccct gcaccggctt gttggcgaag
     5101 ctgggggtca gccagatggc ggtcgtcccc agtccctcga tgtagtcgag ccggtcgcgc
     5161 agtcccttca gatcaccgcc ctggtagaag cccttgtcgg tggggtcgaa gccggtggtc
     5221 aggcggtcgc cggtgaggcc accggtgtcg ttggcggtgt cgccgttcgc gaaccggtcg
     5281 gtcatgacga agtagaactg ctcctcggag ccgggctggc gcaccggctt cgcgatcagg
     5341 gcgtcgtcgt ccgccgtgta cccctggcgg aggccggtcg cctgcacgcc gacgcgcttg
     5401 aggttgtcgt cgaacgtgaa gcgcagggtc gcggggccgg cgaccgtgag ggggatgttg
     5461 tcgccgccgc cgtcgagacc gtaggcctcg tcccacgtgt cgttcagcgc gaccttgtac
     5521 tcgaagctgc cggccggcac cgtgaactcg gccgagtaga tcccggcggc gccggtgggc
     5581 gcgagctcgg tctgggtgca gtccggtgcc cagtcggccg cgcagccgag ctcggactgc
     5641 aggtcgccga cgagggcgac ggt
//
LOCUS       DATMSX010000346         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_137976,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000346 DATMSX010000000
VERSION     DATMSX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137976"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1011)
                     /locus_tag="VN041_10810"
     CDS             complement(67..1011)
                     /locus_tag="VN041_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU29564.1"
                     /translation="MSRVLVTGGSGFLGSHVTALLAQHPSVEVVVSGDLRDSAVPGVI
                     SKRVDVTDAAGIAPVLRRHRIDTVVHLAAIVNPGRDVALEHRVDVEGSRNVLDACLDS
                     GVRRLVVSSSGAAYGYHADSPEWLDERDPVRGNDEFPYSRHKRIVEEMLAEARDEQPE
                     LEQVIFRIGTILGPSVHNQITALWDGARLLRVAGSDSPFVFVWVDDVAAAMVRAATDG
                     PPGVFNVAGDGRMTVPEIAARLGKRMLSVPAWALGAALAVGHALRLTEHGPEKVRFLR
                     YRPVLANRRLKEEFGYTPARTSREAFEEYLHTHPGVAR"
     gene            complement(1008..1976)
                     /locus_tag="VN041_10815"
     CDS             complement(1008..1976)
                     /locus_tag="VN041_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HWU29565.1"
                     /translation="MDFDQLTLNFTPGTLLILNIVLGLIMFGIALDTKPSDFRVVLRH
                     PRPFLIAILAQLLVLPAVTFGLTLILPVSASMALGMILVACCPPGNISQVLTHRSGGN
                     VALSVSLTAVGNLIYIVAMPLSIAFWGSLHPTARTLLHAVALDPWKMLLEIVLIIGLP
                     FVAGLLLRARLPRFAQRVQPFVRWFSLIALVGFIVGALAGNWAVFLSVIGTVLIVVAI
                     HDAVALAIGYSTAAIGGLGTPERKAMTFEVGIRNSGLGLGLIFAFFDGLGGMAVVAGW
                     WGVWDIIAGLALAGLWAMHTRRRTGSARGDASRHAAESDAAEAAAA"
     gene            complement(1976..3247)
                     /locus_tag="VN041_10820"
     CDS             complement(1976..3247)
                     /locus_tag="VN041_10820"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU29566.1"
                     /translation="MKRYAVIGAGPSGLSAARALQLRGINVEGYEASSGVGGLWDIDN
                     PRSTMYESAHLISSRTTTEFAEFPMDSDVDYPGHRALKAYFDSFADAFGLRDLFRFDT
                     RVTRVEHGADGGWMLHAEGLEPRRYDGVILANGTLAEPHIPTFPGEFTGEIMHTSAYK
                     RAGQLAGKRVLIIGAGNSGCDIAVDAVHHAASVEMSVRRGYHFVPRYLFGRPSDTLNQ
                     GRPLPARLKQFIDTRVLKAFTGDPVRFGFPKPDYRIYESHPIVNTLVLNHLGQGDLRV
                     RADVDRFDGRTVHFRDGSSGEYDLILLATGYTLDYPFVDREHLHWEQASPKLFLNVFP
                     ASFNGLYVIGMIEASGIGWQGRFEQARMLAAYLAADEERAAAFRARVGGQPWPDTTGG
                     YRYLGLARMSYYVNKDAYRSAVRAATRSLEA"
     gene            complement(3335..>3614)
                     /locus_tag="VN041_10825"
     CDS             complement(3335..>3614)
                     /locus_tag="VN041_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29567.1"
                     /translation="FALGDDWPQRRFAASAQIVFLGYGAVSVALRGLPATPLPDLAVL
                     AACTAGGMILGAFAARRVPLALARRVMLLCAWAGTLVVLGRATASLIA"
BASE COUNT          586 a         1247 c         1283 g          498 t
ORIGIN      
        1 gctgcgctcc cgccacctgc atccgctgct cgacccgatc ctggcctgag ccctggagtc
       61 ccggcgtcag cgcgcgacgc cggggtgcgt gtgcaggtac tcctcgaacg cctcccgcga
      121 ggtgcgcgct ggcgtgtagc cgaactcttc cttcagacgc cgattggcca gcaccggccg
      181 gtaacgcagg aagcggacct tctccgggcc gtgctcggtg agccggaggg cgtgcccgac
      241 cgcgagcgcg gcacccagcg cccaggcggg gacgctcagc atccgcttgc cgagcctcgc
      301 agcgatctcc ggcaccgtca tgcggccgtc gccggcgacg ttgaagacgc cgggcgggcc
      361 gtcggtggcg gcgcgcacca tggcagctgc gacgtcgtcg acccacacga acacgaaagg
      421 cgagtcggag ccggccacgc gcagcagtcg tgctccgtcc cacagcgcgg tgatctggtt
      481 gtgcacggag gggcccagga tcgtcccgat ccggaagatc acctgctcga gctcgggttg
      541 ctcgtcccgc gcctccgcga gcatctcctc cacgatccgc ttgtgccggg agtaggggaa
      601 ctcgtcgttg ccgcgcacgg ggtcgcgctc gtcgagccac tccgggctgt ccgcgtgata
      661 tccgtaggcc gcacccgagc tggagaccac gaggcggcgc acgccggagt cgaggcacgc
      721 gtccagcacg ttccggctgc cctcgacgtc gacgcggtgc tccagggcga cgtcgcgccc
      781 ggggttcacg atggcggcga ggtgcacgac cgtgtcgatg cggtgccgcc gcagcacggg
      841 tgcgatgccc gcggcatccg tcacgtcgac gcgcttggag atcacgccgg ggacggcgga
      901 gtcgcgcagg tcgccgctga cgacgacctc caccgacgga tgctgcgcga gcagcgccgt
      961 gacgtgagag ccgaggaatc cggacccgcc ggtgaccagc acccggctca cgcggcggcc
     1021 gcctctgcgg catccgattc ggcggcgtgc cgggaggcgt cgccgcgcgc tgaccctgtg
     1081 cggcgcctgg tgtgcatggc ccagagccct gccagcgcca gcccggcgat gatgtcccac
     1141 acgccccacc agcccgcgac caccgccatg cccccgagcc cgtcgaagaa cgcgaagatc
     1201 agtcccagac cgagtccgga gttgcggatg ccgacctcga aggtcatggc cttgcgctcg
     1261 ggcgtgccga ggcccccgat cgcggcggtg gagtagccga tcgccagcgc caccgcgtcg
     1321 tggatcgcca cgacgatcag cacggtgccg atcacgctga ggaacaccgc ccagttgccc
     1381 gcgagcgccc cgacgatgaa gcccaccagc gcgatcaggc tgaaccagcg cacgaacggc
     1441 tgcacgcgct gcgcgaatcg gggcaggcgt gcacgcagca gcagtccggc cacgaacggc
     1501 aggccgatga tcagcacgat ctccagcagc atcttccacg ggtcgagggc cacggcatgc
     1561 agcagggtgc gggcggtcgg atgcagcgag ccccagaagg cgatgctgag cggcatcgcc
     1621 acgatgtaga tcagattccc gaccgcggtc agcgacaccg acagcgccac gttgccaccg
     1681 gagcggtgcg tgagcacctg tgagatgttc ccgggcgggc agcaggccac caggatcatc
     1741 ccgagcgcca tcgacgcgct caccggcagg atcagcgtca gcccgaacgt cacggccggc
     1801 agcaccagca gctgcgcgag gatcgcgatc aagaacggcc gcggatgccg cagcaccacc
     1861 cggaagtcgg acggcttggt gtccagggcg atgccgaaca tgatcagccc gagcacgatg
     1921 ttcaggatga gcagcgtgcc tggcgtgaag ttgagggtga gctggtcgaa gtccatcacg
     1981 cctccaggct gcgggtggcg gcgcgcaccg cggagcggta ggcgtccttg ttgacgtagt
     2041 acgacatccg tgccagaccg aggtagcggt agccgccggt ggtgtccggc cagggctgcc
     2101 ctcccacgcg ggcgcggaag gcagcggcac gctcctcgtc cgccgcgagg taggcggcga
     2161 gcatccgtgc ctgctcgaac ctgccctgcc atccgatgcc ggatgcctcg atcatgccga
     2221 tgacgtacag cccgttgaag gaggcgggga agacgttcag gaagagcttc ggcgaggcct
     2281 gctcccagtg cagatgctcg cggtcgacga acgggtagtc cagcgtgtac ccggtggcga
     2341 gcagaatcag gtcgtactcg ccggaggagc cgtcgcggaa gtgcaccgtg cgcccgtcga
     2401 agcggtcgac gtcggctcgg acgcgcaggt ccccctgccc gaggtggttc agcaccaggg
     2461 tgttcacgat cgggtgcgac tcatagatgc gatagtcggg tttcgggaat ccgaaccgga
     2521 caggatcgcc ggtgaaggcc ttcagcaccc gcgtgtcgat gaactgcttg agccgcgccg
     2581 gaagcgggcg gccctggttg agcgtgtcac tgggcctgcc gaaaaggtac cgcggcacga
     2641 agtggtaacc gcgacgcacg ctcatctcga cggatgccgc gtggtgcacg gcatccacgg
     2701 cgatgtcgca gccggagttg cccgcgccga tgatcagcac gcgcttcccg gccagctgcc
     2761 cggcgcgctt gtaggcgctg gtgtgcatga tctcgccggt gaactccccg gggaatgtgg
     2821 ggatgtgcgg ctcggcgagc gtgccgttgg cgaggatgac gccgtcgtag cggcgtggct
     2881 cgagcccctc ggcgtgcagc atccacccgc cgtcggcgcc gtgctcgacc cgggtgaccc
     2941 tggtgtcgaa gcggaacagg tcgcgcagcc cgaacgcatc cgcgaaggag tcgaagtacg
     3001 ccttcagcgc gcgatggccg gggtagtcga cgtccgagtc catcgggaac tcggcgaact
     3061 cggtggtcgt gcgcgaggag atcagatgcg cggactcgta catcgtgctg cgcgggttgt
     3121 cgatgtccca gagcccgccg acaccggagg atgcctcgta gccctcgacg ttgatgccgc
     3181 gcagctgcag ggcccgggcg gcggacagcc cggagggacc ggcgccgatg acggcgtatc
     3241 gcttcatttc actcccgcac ggcgtcgtgc tcgatcgatg ttccccccgg ttccttgatc
     3301 gtagcggggg gagagcgctt cctggaaccg gggatcaggc gatcagagac gcggtcgcac
     3361 gccccagcac gacgagcgtc cccgcccatg cgcacagcag catcacccgt cgcgccagtg
     3421 ccagcggcac ccgccgtgcg gcgaacgccc ccaggatcat ccctcctgcc gtgcaggcgg
     3481 cgaggaccgc cagatcggga agcggcgtag cgggcagtcc gcgcagggcc accgagaccg
     3541 cgccgtagcc gaggaagacg atttgcgcgc tcgccgcgaa gcgccgctgc ggccagtcgt
     3601 cgcccagcgc gaag
//
LOCUS       DATMSX010000347         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_62606,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000347 DATMSX010000000
VERSION     DATMSX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62606"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VN041_10830"
     CDS             <1..402
                     /locus_tag="VN041_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWU29568.1"
                     /translation="LAVGAAVAGGWLMAGTLKVIVARPRPEWTDAVHRVVAPETDASY
                     PSGHVAFVTALATVLILLTWRTAARGWVLAGGVMLVVVTALARVYAVVHYPSDVVAGA
                     VCAVCGVILGFAAMEMFERRRSRNGGTATAI"
     gene            complement(459..531)
                     /locus_tag="VN041_10835"
     tRNA            complement(459..531)
                     /locus_tag="VN041_10835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(495..497),aa:Glu,seq:ctc)
     gene            complement(608..1633)
                     /locus_tag="VN041_10840"
     CDS             complement(608..1633)
                     /locus_tag="VN041_10840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU29569.1"
                     /translation="MTDIEFGLDTFGGVTHDAEGNPVPHPQVVRDLIEEAVLADGLGL
                     DFFGLGEHHRPDFAVSSPEVVLSAIAARTERIRLGSAVTVLSSDDPVRVFERFSTLDA
                     VSNGRAEIVAGRGSFIESFPLFGFDLRQYEELFEERLDLLSQLLKEEPVTWQGNTRAA
                     LENQRVFPTTANGLRTWVGVGGSPDSVVRTARYNFGLFLAIIGGPAARFAPYIDLFER
                     AQDQFGVEHKPVAVHSPGLVAETDEKARALTYDGWLAMRTRMGQERGWAPPAVGDFER
                     EIESGALYIGSPETVARKIVATLNTLRVNRFDFKYDQSMITHQTMLDSIGLYGEKVVP
                     MVKDMLG"
     gene            complement(1754..1826)
                     /locus_tag="VN041_10845"
     tRNA            complement(1754..1826)
                     /locus_tag="VN041_10845"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Glu,seq:ctc)
     gene            1976..2614
                     /locus_tag="VN041_10850"
     CDS             1976..2614
                     /locus_tag="VN041_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWU29570.1"
                     /translation="MRVTKFEHAALRLDHVGQTLVIDPGSFTAPLDDLHDLVAIVLTH
                     EHADHWTPGHLDRMLRHAPGTPIYAPAGVAEAAGDYDVNVVRPGDTVTVGEFTLRFFG
                     GDHAVIHSSLPHVDNVGVLVNEQFYYPGDSYAVPEGVAVDTLAAPLGAPWLKIGEAID
                     FVLAVAPKRAFGTHDMTLSTIGRTMHQQRLQWATEQGGGEFVDLQPGDALDL"
     gene            complement(2640..>3098)
                     /locus_tag="VN041_10855"
     CDS             complement(2640..>3098)
                     /locus_tag="VN041_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HWU29571.1"
                     /translation="ALATTGDATVEEIIAERAADDTATGRTAEIRALASRPDAATRAA
                     AWDSAWNDLTLSNDHLDATIGGFRAGGRRDLIASFDGEYFERIREVWGTRSIELAQRL
                     VVGLFPATTDTTLVDAWLEANSDAPGALRRLVIESRDDLAREIRVRAAQG"
BASE COUNT          474 a         1071 c         1050 g          503 t
ORIGIN      
        1 ctggctgtcg gcgcggccgt cgccggcggc tggctgatgg ccggcacact gaaggtgatc
       61 gtcgctcggc cccgtccgga gtggacggat gccgtgcatc gcgtcgtcgc accggagacc
      121 gatgcctcct acccgagcgg acacgtcgcc ttcgtcacgg cgttggcgac cgtgctgatc
      181 ctgctgacgt ggcgcacggc cgcgcgcggc tgggtgctcg cgggcggcgt catgctggtg
      241 gtggtcactg cgctcgcccg cgtgtacgcc gtggtgcact atccctcgga cgtcgtcgcg
      301 ggcgcggtgt gcgcggtctg cggcgtcatc ctggggttcg ctgcgatgga gatgttcgag
      361 agaagaagat ctcggaacgg tggcaccgcg acggcgatct gagccgagcg caccgaaagg
      421 aagaaggccc tccggattcc ggagggcctt ctggtgagtg accccagcgg gattcgaacc
      481 cgcgttaccg ccgtgagagg gcggcgtact aggccgctat acgatggggc cgtcactgca
      541 accgttcaag tatgccatga ccgcgaaggc cccgccaaat cgaggcggga cctccgcaga
      601 acgcccgtca gccgagcatg tccttgacca tcgggacgac cttctcgccg tacaggccga
      661 tcgaatccag catggtctgg tgcgtgatca tgctctggtc gtacttgaag tcgaagcggt
      721 tcacgcgcag ggtgttcagc gtcgcgacga tcttgcgggc cacggtctcg ggcgacccga
      781 tgtacagcgc accgctctcg atctcgcgct cgaagtcgcc gaccgccggc ggcgcccatc
      841 cgcgctcctg ccccatgcgc gtgcgcatgg cgagccatcc gtcgtaggtc agcgcgcgtg
      901 ccttctcgtc ggtctcggcg acgaggcccg gcgagtgcac ggcaaccggc ttatgctcga
      961 caccgaactg atcctgcgca cgctcgaaca ggtcgatata cggcgcgaag cgcgcggcgg
     1021 gccctccgat gatcgcgagg aacaggccga agttgtagcg cgcggtgcgc acgacggagt
     1081 cggggctgcc accgacgccc acccaggtgc gcagcccgtt cgcggtggtc gggaagacgc
     1141 gctggttctc gagagccgcg cgggtgttcc cctgccaggt gacgggctcc tctttcagca
     1201 gctgcgagag caggtcgagg cgctcctcga acagttcctc gtactgtcgc aggtcgaacc
     1261 cgaagagcgg gaaggactcg atgaacgagc cacgtcctgc gacgatctcc gctcggccgt
     1321 tcgagaccgc gtcgagagtc gagaagcgct cgaacacgcg cacggggtca tcggagctca
     1381 gcaccgtgac agcggagccg agccggatgc gctccgtgcg tgcggcgatc gccgacagca
     1441 ccacctcggg gctggacacg gcgaagtccg gccggtggtg ctcccccagt ccgaagaagt
     1501 ccaggcccag tccgtcagcg agcacggctt cctcgatgag atcgcgcacc acctgcggat
     1561 gcgggacagg gttgccctct gcgtcatggg tcacgccgcc gaaggtgtcg agcccgaatt
     1621 cgatgtccgt catgctcacg acaacctcct ccccgggaat ttcattccga tgcatagatc
     1681 gatgtctgtg ctgaacaggc cgctcggaaa tgcagaaggc cctccgatcg ctcggagggc
     1741 cttcgtgtcg ctgtgacccc agcgggattc gaacccgcgt taccgccgtg agagggcggc
     1801 gtactaggcc gctatacgat gaggccgttc tggcccttgc gggccgtttt ggcaaccgtg
     1861 caagtctgcc acggcgcatc ctgcgaatcc aaatcagggc tcctcttctc ggtccgacgg
     1921 gcgtgtcgcg gcatccggct tgctctcgca cgagcgacgg agttgactcg gcgacatgcg
     1981 cgtcaccaag ttcgaacatg ccgccctccg cctcgaccac gtcggccaga cgctcgtgat
     2041 cgacccgggc tcgttcaccg ctcccctgga cgatctgcac gacctcgtgg cgatcgtgct
     2101 cacccacgaa cacgccgacc actggacacc gggtcacctc gaccggatgc tgcgccatgc
     2161 ccccggcacg cctatctacg ctccggcggg cgtcgccgag gccgcgggtg attacgacgt
     2221 caacgtcgtc cggcccggcg acacggtcac ggtaggcgag ttcacgctgc gcttcttcgg
     2281 cggcgatcat gccgtgatcc actcatccct gccgcacgtc gacaatgtcg gcgttctcgt
     2341 caacgagcag ttctactacc ccggagactc ctacgccgtg cccgagggcg tcgccgtgga
     2401 cacgctcgcg gcgccgctcg gcgcgccgtg gctgaagatc ggcgaggcca tcgacttcgt
     2461 gctcgcggtc gctccgaagc gggcgttcgg cacgcacgac atgaccctgt ccacgatcgg
     2521 gcgcaccatg catcagcagc ggctgcagtg ggcgaccgag cagggtggag gcgagttcgt
     2581 cgatctccag cccggggacg ccctggacct ctgacccccg gctcttcgag ccctgagggt
     2641 cagccctgcg cggcccgcac ccggatctcg cgcgcgaggt cgtcgcgcga ctcgatcacc
     2701 aggcgccgca gcgcaccggg cgcgtcggag ttcgcctcca gccacgcgtc cacgagggtc
     2761 gtgtcggtcg tcgccgggaa aagccccacg acaaggcgct gcgcgagctc gatcgagcgg
     2821 gtgccccaca cctcgcggat ccgctcgaag tactcgccgt cgaaggaggc gatcagatcc
     2881 cggcgcccgc cggcccggaa gccgccgatc gtcgcatcca ggtgatcgtt gctgagggtg
     2941 aggtcgttcc aggcggagtc ccacgccgcc gcccgcgtcg ccgcatccgg gcgggacgcc
     3001 agcgcgcgga tctccgcggt gcggccggtg gcggtgtcgt cggcggcgcg ctcggcgatg
     3061 atctcctcga cggtcgcgtc gcccgtggtg gcgagggc
//
LOCUS       DATMSX010000348         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_52619,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000348 DATMSX010000000
VERSION     DATMSX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52619"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..351)
                     /locus_tag="VN041_10860"
     CDS             complement(10..351)
                     /locus_tag="VN041_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HWU29572.1"
                     /translation="MARFLIIGGFLAVVFWVFSIVDCSVQPATRHRGVSKTAWVLIVL
                     LLPMIGGILWFALGRLRADEVNTAYVVPPDDDPRVLRRMHDAEQDARIRELEAELARL
                     DDDPDGADPRS"
     gene            445..705
                     /locus_tag="VN041_10865"
     CDS             445..705
                     /locus_tag="VN041_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HWU29573.1"
                     /translation="MKKLPPLLVYSVLRLLAFLVPLAIMWFFFPIFQEFWWLAALFAA
                     LIGMSISLLFLRAPLSDASRDIYARRNRTDTDADAEDRATDE"
     gene            complement(788..1429)
                     /locus_tag="VN041_10870"
     CDS             complement(788..1429)
                     /locus_tag="VN041_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU29574.1"
                     /translation="MAVRVDLNISLDGFATTVDQTPENPMGEDWGRLTAAYVSTRTFR
                     ERVMGDTSGGGTTGVDEKYASAYFEGTGAEIMGAGMFGLHLFGDDPDWRGWWGEEPPF
                     QVPVFVLTHTAKPSIEFANGTEFHFIDTTPEDALARATEVAAGGDVRVGGGIDVVRQF
                     LRANLVDRLHVAIAPILLGRGERLWDDLRGLEQGYEVVSEVAESGTTHVTFRR"
     gene            complement(1438..1731)
                     /locus_tag="VN041_10875"
     CDS             complement(1438..1731)
                     /locus_tag="VN041_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HWU29575.1"
                     /translation="MSFQAYLDNIETKTGLTPRQFIELATQRGFDSTTKATPILEWLK
                     SDYDLSRGYGMALVHVITKGPQISDKHVGSTGSHGDASDVLWLDGKATNPANG"
     gene            complement(1743..2096)
                     /locus_tag="VN041_10880"
     CDS             complement(1743..2096)
                     /locus_tag="VN041_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU29576.1"
                     /translation="MIDTLAEPTLVAPAQIPLGAGPGRRFEGRDHGAAVSYFYVDNQP
                     GKGSGPHWHPYPETWVVLEGDATFQVGDEQIVATAGDTVTGPAFVPHRFTNTGTGTMR
                     LIGIHASAVIIQTDL"
     gene            2164..3195
                     /locus_tag="VN041_10885"
     CDS             2164..3195
                     /locus_tag="VN041_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HWU29577.1"
                     /translation="MDRNVQRRPVEGREHPDHRVDFHLSPGDIQAVRFGISPGHEFAH
                     AVRILLRPEQHPLQWGWLRQVRDRLPREPFGLLAQVIGDDGYMPDFLTADARWDMTPA
                     DELAALREAPIPGILVDLGKMIVRSSGDRRAALVRMHADPVRARALIADAWDQVWDAV
                     LAPVWPQLERLLRADIAVRARTITTDGLSAMAGALHPNVRWSDGAVRVRLRRHSEDVD
                     CRGSGLVLVPSVMSSWGCMVITEAPAQPTLFYPARGVTAGWARDSAELEDALGALLGP
                     ARAGILLRAHVARTTTGIAKDAGIAASTASHHLTVLRDAGLIASSREGNRMLHLRTPL
                     AEALVGAAL"
     gene            complement(3197..>4027)
                     /locus_tag="VN041_10890"
     CDS             complement(3197..>4027)
                     /locus_tag="VN041_10890"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HWU29578.1"
                     /translation="PLAVAPVVLGTGAAQVTDPHLHWVIALACLAVAVLLQIGVNFAN
                     DYSDGIRGTDAHRVGPARLTASGRVSPKQVLTTALVFFALAAVAGLAIVIRTQQWWML
                     IVGAACIVAAWFYTGGKRPYGYNAMGELFVFVFFGLVAVLGTTWVQSFALPLEAWLAA
                     VAAGLFACAVLLANNLRDIDQDRAVGKRTLTVLIGKRATQIVYTVFVIAPFVIAAVFA
                     LLLYPVAWLSLLVLLAIIPAILIVWTYRTPRELIIALSLTSLSSVGYAALMYWALVAA
                     "
BASE COUNT          616 a         1414 c         1379 g          618 t
ORIGIN      
        1 cgggccccgt cacgagcgcg ggtccgcgcc gtcggggtcg tcgtccaggc gcgccagctc
       61 ggcctcgagc tcgcggatgc gggcatcctg ctcggcgtcg tgcatgcggc gcagcacccg
      121 cggatcatcg tccggcggca ccacataggc cgtgttcacc tcgtcggcgc ggagcctgcc
      181 gagtgcgaac cagaggatgc cgccgatcat cggcagcagc agcacgatga gcacccaggc
      241 ggtcttcgag acgccacggt gccgcgtcgc cggctgcacg ctgcagtcga cgatgctgaa
      301 cacccagaac accacggcga ggaaaccgcc gatgatcagg aaacgcgcca tgcttccagt
      361 gtaggtcgcc gggcccggac gggccccggg acctcatcgc cgccgcacag cgcacgccta
      421 gcgcgcgctc ctagactctc ctctgtgaag aagttgccac ccctcctcgt gtactccgtg
      481 ctgcggctgc tggcgttcct cgtaccgctc gcgatcatgt ggttcttctt cccgatcttc
      541 caggagttct ggtggctggc ggccctgttc gccgcgctga tcggcatgag catctccctg
      601 ctgttcctgc gcgcgccgct gtccgacgcg tcgcgcgaca tctacgcccg tcgcaaccgc
      661 acggacaccg atgcggatgc cgaggatcgc gcgaccgacg aatgaaccgc aggcggtgag
      721 atgcagaagg agcggatgcc gatcggcatc cgctccttct gcgtcagcgc ctcagcgcct
      781 cagcgcctca gcgccggaag gtcacgtgcg tcgtgccgct ctccgcgacc tcgctgacca
      841 cctcgtaacc ctgctcgagg ccgcgcaggt cgtcccacag ccgctcgccg cggccgagca
      901 ggatcggcgc gatcgcgaca tgcagccgat cgacgaggtt cgcgcgcagg aactggcgca
      961 cgacgtcgat gcctccgccg acgcgcacat cgcctcccgc ggcgacctcg gtcgcccgcg
     1021 ccagggcatc ctccggggtc gtgtcgatga agtggaactc ggtgccgttc gcgaactcga
     1081 tcgacggctt cgcggtgtgc gtgagcacga acaccggcac ctggaacggg ggctcctcgc
     1141 cccaccagcc ccgccagtcg gggtcgtcgc cgaacaggtg cagcccgaac atgccggccc
     1201 ccatgatctc ggcacctgtc ccctcgaagt aggcgctcgc gtacttctcg tcgacgcccg
     1261 tcgtgccccc gccggaggtg tcgcccatca cccgctcgcg gaaggtgcgc gtggagacgt
     1321 acgcggcggt cagccgcccc cagtcctccc ccatcggatt ctccggcgtc tggtcgacgg
     1381 tggtcgcgaa cccgtcgagc gagatgttca ggtctactcg tactgccatg gtctctccta
     1441 gccgttcgcg ggattcgtgg ccttgccgtc cagccacagc acgtccgagg cgtcaccatg
     1501 cgatccggtg ctcccgacgt gcttgtcact gatctgcggc cctttggtga tcacgtgcac
     1561 gagcgccatt ccgtagccgc gcgacagatc gtagtcggat ttcagccact ccaggatggg
     1621 tgtggccttg gtggtggagt cgaagccgcg ctgcgtcgcc agttcgatga actggcgcgg
     1681 ggtgaggccc gtcttcgtct cgatgttgtc gaggtatgcc tggaaggaca tgtccgctct
     1741 cctcagaggt ctgtctggat gatgacggcg gaggcgtgga tgccgatcag gcgcatcgtg
     1801 ccggtgccgg tgttggtgaa ccggtgcggc acgaacgccg gaccggtgac ggtgtcaccc
     1861 gcagtggcga cgatctgctc atcgcccacc tggaaggtcg cgtccccttc gagcacgacc
     1921 caggtctcag gatacggatg ccagtgcggc cccgacccct tccccggctg gttgtcgacg
     1981 tagaagtacg acaccgcggc cccgtggtca cggccctcga agcggcgacc gggaccggct
     2041 ccgagcggga tctgggcggg tgcgacgagc gtcggctcgg cgagtgtgtc gatcatgatc
     2101 tgctccctgt cgtgtgcatg ccccactctt ccgccgcgtg cgacgcagcc ctagagtttc
     2161 gatgtggatc gaaacgttca gcgccggccg gtggagggtc gcgagcaccc cgatcaccgc
     2221 gtcgacttcc acctgagccc cggtgacatc caggcggtcc gcttcggcat ctcgcccggt
     2281 cacgagttcg cgcacgcggt gcggatcctg ctgcggcccg agcagcatcc gctgcagtgg
     2341 ggctggctgc gccaggtgcg cgaccggctg ccgcgagagc cgttcggcct gctggcccag
     2401 gtgatcggcg acgacggcta catgcccgac ttcctcaccg cggacgcgag gtgggacatg
     2461 acgcctgccg atgagctcgc cgccctgcgc gaggcgccga tccccggcat cctcgtcgat
     2521 ctcggcaaga tgatcgtgcg ctcctccggc gatcgccggg ccgcgctggt gcggatgcac
     2581 gcagaccccg tccgtgcccg agcgctgatc gccgacgcct gggatcaggt gtgggatgcc
     2641 gtgctcgccc ctgtctggcc gcagctggaa cgactgctgc gcgccgacat cgcggtgcgg
     2701 gcgcgcacga tcaccacgga cggcctctcc gcgatggcgg gcgcgctgca cccgaacgtg
     2761 cgatggagcg acggcgccgt gcgggtgcgc ctgcgccggc acagtgagga cgtggactgc
     2821 cgcggctccg ggctggtgct cgtcccgtcg gtgatgtcgt cgtggggatg catggtgatc
     2881 accgaggcgc ccgcgcagcc gacgctgttc taccccgcgc gcggagtgac ggcggggtgg
     2941 gcgcgagaca gtgcggagct cgaggacgcc ctgggcgcgc tgctcggtcc cgcgcgtgcg
     3001 ggaatcctgc tgcgggcgca cgtcgcgcgc accaccaccg gaatcgcgaa ggatgccggg
     3061 atcgccgcgt ccacggcatc ccaccacctg acagtgctcc gcgacgcagg cctgatcgcc
     3121 agctcgcgcg agggcaatcg gatgctgcac ctgcgcaccc cgctcgcaga ggcgctggtc
     3181 ggcgcggcgc tctgactcag gccgcgacga gcgcccagta catcagtgcg gcgtatccga
     3241 ccgacgacag cgaggtcagg ctcagcgcga tgatcagctc acgaggggtc cggtacgtcc
     3301 agacgatgag gatcgccggg atgatcgcca gcagcaccag cagtgacagc cacgcgacgg
     3361 ggtacagcag cagcgcgaac actgccgcga tcacgaaggg cgcgatcacg aagacggtgt
     3421 agacgatctg cgtggcacgc ttgccgatca gaaccgtgag cgtgcgcttg ccgacggcgc
     3481 gatcctgatc gatgtcgcgc aggttgttgg ccagcagcac ggcgcaggcg aacagaccgg
     3541 cggcaaccgc agcgagccac gcctccagcg gcagcgcgaa ggactgcacc cacgtggtgc
     3601 cgagcaccgc caccagcccg aagaacacga agacgaacag ctcgcccatc gcgttgtagc
     3661 cgtacgggcg cttgccgccg gtgtagaacc aggcagcgac gatgcacgcc gcgccgacga
     3721 tgagcatcca ccactgctgc gtgcggatca cgatcgcgag ccccgcgact gcggcgagag
     3781 cgaagaacac cagggcggtg gtcagcacct gcttcggtga gacgcggccg gacgccgtga
     3841 gccgggcagg gccgacccga tgggcgtcgg tgccgcggat gccgtcgctg tagtcgttcg
     3901 cgaagttcac gccgatctgc agcagcacgg cgaccgccag gcacgccagc gcgatcaccc
     3961 agtgcaggtg gggatcggtg acctgcgctg cgcccgtgcc gaggacgacc ggcgcgacag
     4021 ccagggg
//
LOCUS       DATMSX010000349         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166181,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000349 DATMSX010000000
VERSION     DATMSX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166181"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..827)
                     /locus_tag="VN041_10895"
     CDS             complement(111..827)
                     /locus_tag="VN041_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HWU29579.1"
                     /translation="MSSVLYVCVRPERSAADAEHRSFARDLGIPQLDRLDLLHASLET
                     VEWQQYNGFVIGGSPFNVTDAVKDETQQTVERDLERIADRALTGAATALFTCYGIGVV
                     TRMLGGRVTMEHPERVQAARIRLTADGTEDPLFGPNAPSMSVFTAHKESAAETPPDAV
                     LLATKDDCPVQAYRVGEHLYATQFHPEASPQDFADRMAVYRASGYFDPSEYAATERMV
                     LSASVDGADLLGRFPTLLGI"
     gene            928..3477
                     /locus_tag="VN041_10900"
     CDS             928..3477
                     /locus_tag="VN041_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWU29580.1"
                     /translation="MPNDFNEAGSGSFDEFLARYLAGEQARQARSIDLSRFLTARTQQ
                     ILQRAGRFALERGQTELDALHVLRVIVADEAVAEAISRIGVDPAAIVSAVDARLPMPG
                     ESSDDGAATITPSASRVLFHAYQVARSSGSTYIEPEHVFFALVIGQDAPSGQILARAG
                     VTAEALTQSLRGEPVHAGVAPQDESAENPASETPMLDKYGLDLTARARAGELDPVIGR
                     SVEIEQTIEILSRRTKNNPVLIGEAGVGKTAIVEGLARAIVEGAVPAALKDRRVVSLD
                     LPGMLAGTRYRGDFEERLTKTMDEISAHKGEIIVFLDELHTVVGAGGSGEGGTDAGNI
                     LKPRLARGDLHLVGATTLSEYRRIEKDPALERRFQPVKVGEPSIEDAVRILQGLKPAY
                     QEHHDVTFTDESIRAAVELSARYLPDRVLPDKAIDLIDQAGARLRLRLGVVPDVSALM
                     AELADLESRKNAAVGAEHYEEASRIRDEITDVQRRIDESRTSVSGSASGEAVVDEAQI
                     AAVISRATGIPVSRLTESERSRLHELEADLHARVIGQDDAVTAVARAVRRSRTGMGDA
                     RRPVGSFLFLGPTGVGKTELAKALADRLFDDENAVIRFDMSEFGERHTVSRLVGAPPG
                     YVGYDEAGQLTERVRRNPYSIVLFDEIEKAHPDVFNLLLQVLDDGRLTDGQGRTVDFR
                     NTVIVMTSNIGSEFLASRSGALGFVASQDASGFASQADLKARVMGKLREAMRPEFLNR
                     IDEIVIFQKLSQVELGEIVRLMLQATAARLAGREVSFEVTDAAVAWLGEHGYEPEYGA
                     RPLRRLIQNEVDDRIADLFVVGSLADGGRVVVDAADGGLIVTSGAHLATAA"
     gene            complement(3566..4288)
                     /locus_tag="VN041_10905"
     CDS             complement(3566..4288)
                     /locus_tag="VN041_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HWU29581.1"
                     /translation="MKNDRRRPARPRPGKPSPKKAQQKPAPRKAAPKKPSPQPRPAPE
                     EPRILRLGLVPGTTPGRWIDTWKERMPHVELELVPMSFAEQREQLAEVDLALVRLPVA
                     RADDLHVIPLYDELPVVIAAKESHLMAADELSPDDLAGEVLITPGDDVLGPLDLPTVA
                     PRFPTIETTEDAVATVASGVGILVVPMSLARLHKRKDADYRILRDGPTSTVALAWPRD
                     VTTPDIETFVGIVRGRTANSSR"
     gene            complement(4326..>4640)
                     /locus_tag="VN041_10910"
     CDS             complement(4326..>4640)
                     /locus_tag="VN041_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU29582.1"
                     /translation="VADVAHDEGLQIAASAPRPAEPAEDLDRVSAVLAAQGFEPHEED
                     GALMLANCPFDSLARDHTALVCSANLALIGGILEGMDCTHLQAHGEPHVGQCCVAIRA
                     EV"
BASE COUNT          678 a         1585 c         1682 g          695 t
ORIGIN      
        1 gccggcggtg cgctggtcgg cgacgggcgg gctgatgccg ctggccggct acctggacga
       61 gaggatcgcc ctgctgcaga gctgagcgct gcgccgcccg ggagggagtc tcagatcccg
      121 agcagggtgg gaaaccgccc cagcaggtcg gccccgtcga cggatgccga cagcaccatc
      181 cgctcggtcg ccgcgtactc gctcgggtcg aagtagccgg acgcgcggta caccgccatc
      241 cgatcggcga agtcctgcgg tgacgcctcg gggtggaact gcgtggcgta caggtgctcg
      301 ccgacgcggt acgcctggac ggggcagtcg tccttggtgg ccagcagcac ggcatccggc
      361 ggcgtctcgg ccgcggactc cttgtgagcg gtgaacacgc tcatcgacgg cgcgttcggg
      421 ccgaagagcg gatcctccgt gccgtcggcg gtgaggcgga tgcgcgccgc ctgcacgcgc
      481 tcgggatgct ccatggtgac gcggccgccc agcatccgcg tcacgacgcc gatcccgtag
      541 caagtgaaca gcgccgtggc ggcgccggtc agggcgcgat ccgcgatgcg ctccaggtcg
      601 cgttcgaccg tctgctgcgt ctcgtccttc acggcatccg tgacgttgaa cggcgagccg
      661 ccgatcacga agccgttgta ctgctgccac tcgacggtct ccagcgacgc gtgcagcagg
      721 tcgaggcgat ccagctgcgg gatgccgagg tcgcgggcga aggagcgatg ctcggcgtcg
      781 gccgcgctgc gctccgggcg cacgcagacg tagagcaccg aggacatgcg atcgagtcta
      841 ttcgcggatt tgcgctcgac cgggaatagg cgtcatcatc tgatgttgaa cttgacagaa
      901 gtacgctcaa gtttgagagg aaaccccatg cccaacgact tcaacgaggc gggctcgggc
      961 tcgttcgacg agttcctggc ccgttacctc gcaggtgagc aggcccggca ggcgcgatcg
     1021 atcgacctca gccgcttcct caccgcgcgc acccagcaga tcctgcagcg tgccggtcgt
     1081 ttcgcgctcg agcgcggtca gaccgaactc gatgcgctgc acgtcctgcg cgtgatcgtc
     1141 gccgacgagg ccgtcgccga ggcgatctcc cggatcggcg tcgaccccgc ggccatcgtg
     1201 agtgccgtgg atgcgcgcct gccgatgccc ggcgagtcct ccgacgacgg cgccgccacc
     1261 atcactccga gtgccagccg ggtgctgttc cacgcatacc aggtggcccg ttcctcgggc
     1321 tccacctaca tcgagcctga gcacgtcttc ttcgcgctcg tgatcggtca ggacgccccg
     1381 tcgggccaga tcctcgcgcg tgccggcgtg accgccgagg cgctcacgca gagcctgcgc
     1441 ggggagccgg tgcacgccgg cgtcgcgccg caggacgagt cggccgagaa cccggcatcc
     1501 gagaccccga tgctcgacaa gtacggcctc gacctcacgg cgcgcgcacg cgcgggcgaa
     1561 ctcgaccccg tgatcggccg cagcgtcgag atcgagcaga cgatcgagat cctcagccgt
     1621 cgcaccaaga acaacccggt actgatcggt gaggccggcg tcggcaagac ggcgatcgtc
     1681 gagggactgg cccgcgcgat cgtcgagggc gcggtgcccg ccgcgctgaa ggaccgccgc
     1741 gtggtgtcgc tcgacctgcc cggcatgctc gccggcaccc gctaccgcgg cgacttcgag
     1801 gagcgtctca ccaagaccat ggacgagatc tccgcccaca agggcgagat catcgtgttc
     1861 ctcgacgagc tgcacaccgt ggtcggcgcg ggcggctctg gcgagggcgg cacggatgcc
     1921 ggaaacatcc tcaagccccg cctggcccgc ggtgatctgc acctcgtcgg tgcgaccaca
     1981 ctgagcgagt accgccggat cgagaaggat ccggcactgg agcgccggtt ccagccggtg
     2041 aaggtcggcg agccgtccat cgaggacgcc gtccgcatcc tgcaggggct gaagcccgcc
     2101 tatcaggagc accatgacgt gaccttcacc gacgagtcga tccgtgcggc cgtcgagctg
     2161 agcgcacgct acctgcccga ccgggtgctg cccgacaagg cgatcgacct gatcgatcag
     2221 gccggtgccc ggctgcgcct gcgcctcggc gtcgtgcccg acgtgagcgc gctgatggcg
     2281 gagctggccg acctcgagtc gcgcaagaac gccgcggtcg gcgccgagca ctacgaggag
     2341 gcctcccgca tccgcgacga gatcaccgac gtgcagcgcc ggatcgacga gtcgcgcacg
     2401 tctgtctccg gttccgcctc gggtgaggcc gtggtcgacg aggcgcagat cgccgccgtg
     2461 atctcgcgcg ccaccggcat ccccgtcagc cggctgaccg agagcgagcg gtcgcggctg
     2521 cacgagctgg aggccgatct gcacgcccgt gtgatcgggc aggacgacgc ggtgaccgcg
     2581 gtcgcgcggg ccgtgcgtcg cagtcggacc gggatgggcg acgcccgccg cccggtcggc
     2641 tcgttcctgt tcctggggcc caccggcgtc ggcaagaccg agctggccaa ggccctggcc
     2701 gatcgcctgt tcgacgacga gaacgccgtg atccgcttcg acatgagcga gttcggcgag
     2761 cggcacacgg tgtcgcgtct ggtcggcgcc cctccgggat acgtcggcta tgacgaggcc
     2821 ggccagctca ccgagcgcgt gcggcgcaac ccgtactcga tcgtgctgtt cgacgagatc
     2881 gagaaggcgc accccgacgt gttcaacctg ctgctgcagg tgctcgacga cggccgtctg
     2941 accgacggcc aggggcgcac ggtcgacttc cgcaacacgg tgatcgtgat gacctcgaac
     3001 atcggctccg agttcctggc ctcgcggtcg ggcgcgctcg ggttcgtggc gtcgcaggat
     3061 gcctcgggct tcgcgtccca ggcggatctg aaggcgcggg tgatgggcaa gctgcgggag
     3121 gcgatgcgtc cggagttcct gaaccgcatc gacgagatcg tgatcttcca gaagctgtcc
     3181 caggtggagc tcggcgagat cgtgcggctc atgctgcagg cgaccgccgc gcggctcgcc
     3241 ggtcgcgagg tgtcgttcga ggtgacggat gccgcggtgg catggctggg cgagcacggc
     3301 tacgagccgg agtacggcgc gcgcccgctg cgccggctga tccagaacga ggtggacgac
     3361 cgcatcgcgg acctgttcgt cgtgggatcg ctggccgacg gcggccgggt ggtcgtggac
     3421 gccgccgacg gcggtctcat cgtgacctcc ggcgctcacc tggcgacggc cgcctgatcg
     3481 gctcgacttc agctggttcg aatggccgct tcaccctcgg tgaggcggcc attcccctga
     3541 cttgccgcgg atgctgagcc gagggtcagc gcgaggagtt cgcggtgcgc ccgcgcacga
     3601 tgccgacgaa ggtctcgatg tcgggcgtgg tgacgtcacg cggccaggcg agcgcgacgg
     3661 tcgaggtcgg gccgtcgcgg aggatgcggt agtcggcgtc cttgcgtttg tgcagtcgcg
     3721 cgagcgacat cggcacgacc aggatcccga ctccggaggc gacggtcgcg accgcgtcct
     3781 cggtggtctc gatcgtgggg aaccgcgggg cgacggtggg gaggtccaag gggccgagca
     3841 cgtcatcgcc cggggtgatc agcacctccc cggcgaggtc gtcgggggag agctcatccg
     3901 cggccatcag atgcgactcc ttcgccgcga tgaccacggg cagctcgtcg tagagaggga
     3961 tcacgtgcag gtcgtccgca cgggccaccg gcagccgcac gagcgcgaga tcgacctcgg
     4021 cgagctgctc gcgctgctcg gcgaacgaca tcggcaccag ctcgagctcg acgtgcggca
     4081 tccgctcctt ccaggtgtcg atccacctgc cgggcgtcgt gccggggacc agcccgaggc
     4141 gaaggatccg cggctcctcg ggcgccggtc ggggctgggg cgacggcttc ttcggcgcgg
     4201 ccttccgcgg tgcgggcttc tgctgcgcct tcttcggcga cggcttgccc gggcgcggac
     4261 gtgcagggcg gcgccggtcg ttcttcattg cttcagcgta acgaggtcgc gagcggatgc
     4321 ttcgctcaga cctcggcgcg gatcgccacg cagcactgac ccacgtgcgg ctcgccgtgc
     4381 gcctgcaggt gcgtgcagtc catgccctcg aggatgccgc cgatcagcgc gaggttggcc
     4441 gagcacacca gggcggtgtg atcgcgggcc agcgagtcga acgggcagtt ggcgagcatg
     4501 agcgcgccgt cctcttcgtg cggctcgaag ccctgggccg cgagcaccgc ggagacgcga
     4561 tcgaggtcct ccgcgggctc ggcgggtcgg ggagcggatg cggcgatctg caggccctcg
     4621 tcatgggcga cgtccgcgac
//
LOCUS       DATMSX010000350         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_138019,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000350 DATMSX010000000
VERSION     DATMSX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138019"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VN041_10915"
     CDS             <1..371
                     /locus_tag="VN041_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU29583.1"
                     /translation="LEPGPVIAGDWSAASGYTATAAIIDAGVTAVFCANDQMALGLLG
                     GLHEAGVRVPRDISVVGFDDIPDAAYYWPQLTTVRQDFAELARRAVAVVIADADAAAS
                     ALRPVAPVLVRRDSVARPRA"
     gene            478..1212
                     /locus_tag="VN041_10920"
     CDS             478..1212
                     /locus_tag="VN041_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HWU29584.1"
                     /translation="MSKEAPDFSPELQAAIQSVREDVSRLHGELVRYGLVVWTGGNVS
                     GRVRVGDDPNADLFVIKPSGVSYDDLTPDNMILCDLDGEVIPGTPGSDRSPSSDTAAH
                     AYVYRHMPEVGGVVHTHSDYAVAWAARGEEIPCVITAMADEFGGPIPIGPFAIIGDDS
                     IGRGIVDTLTGHRSRAVLMQNHGPFTIGVNAKDAVKAAVMVEDVARTVHLAREAGPLI
                     PIPQEAIDSLYNRYQNVYGQSADARR"
     gene            1209..>1850
                     /locus_tag="VN041_10925"
     CDS             1209..>1850
                     /locus_tag="VN041_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWU29585.1"
                     /translation="MSAATGHSAREDILAGRTSLGIELGSTRIKACLIGSSAAEVLAT
                     GSFSWENRLQDGLWTYSLDEVWTGLQSAFADLIADVHDRYGVRPDTFGAIGVSAMMHG
                     YLAFDAAGDLLAPFRTWRNTNTGPAAAELTELLGVNIPLRWSVAHLRQAQLDAEPHAG
                     EVRFLTTLAGYVHWRLTGRRVLGIGDASGMFPIDSAVRDYDQKMIARYDERAGS"
BASE COUNT          271 a          672 c          626 g          281 t
ORIGIN      
        1 ggctggagcc ggggccggtc atcgcggggg actggtcggc ggcatccggc tatacggcga
       61 ccgccgcgat catcgacgcc ggcgtcaccg ccgtgttctg cgcgaacgac cagatggcgc
      121 tcggtctgct cggcgggctg catgaggctg gggtgcgggt gccccgcgac atcagcgtgg
      181 tcggcttcga cgacatcccg gatgccgcgt actactggcc gcagctgacg accgtgcggc
      241 aggacttcgc ggagctggcc cgcagagccg tcgccgtcgt gatcgcggac gcggatgccg
      301 cggcatccgc tcttcgtccc gtcgcccccg tgctggtgcg ccgcgactcg gtcgctcgtc
      361 ctcgcgcctg acgtagcggc gagctgcgca cgtgatgttg acggcggcgc gcgagggtgt
      421 catgctgggc gatgtgaccg gtcacggtgg ccggatcatc agatcgatga ggtgcccgtg
      481 agcaaggaag cccccgactt cagccctgag ctgcaggccg cgatccagtc cgtccgcgag
      541 gacgtctccc gtctgcacgg agagctcgtc cgctacggcc tcgtggtgtg gaccggaggg
      601 aacgtctccg gtcgcgtccg cgtcggcgat gatccgaatg ccgacctgtt cgtcatcaag
      661 ccctccggtg tctcgtacga cgacctgact ccggacaaca tgatcctctg cgacctggac
      721 ggcgaggtga tcccgggcac tcccggcagc gaccgctcgc cctccagcga cacggccgcg
      781 cacgcctacg tgtaccggca catgcccgag gtcggcggcg tcgtgcacac gcactccgac
      841 tacgccgtgg cctgggcggc acgcggcgag gagatcccgt gcgtgatcac cgcgatggcc
      901 gacgagttcg gcggcccgat cccgatcggc ccgttcgcga tcatcggaga cgactcgatc
      961 ggccgcggga tcgtcgacac cctcaccggg caccgctccc gtgcggtgct gatgcagaat
     1021 cacggaccgt tcacgatcgg cgtgaacgcg aaggatgccg tgaaggccgc ggtcatggtc
     1081 gaggacgtcg cccgcaccgt gcacctcgcc cgcgaagccg gtccgctcat ccccatcccg
     1141 caggaggcca tcgacagtct ctacaaccgg tatcagaacg tgtacggcca gagcgcggac
     1201 gcacgtcgat gagcgccgca acgggacact ccgctcgcga ggacatcctc gccggccgca
     1261 ccagcctggg tatcgagctc ggctccaccc gcatcaaggc ctgcctgatc ggctcctccg
     1321 ccgccgaggt gctcgccacc ggctcgttct cctgggagaa ccggcttcag gacgggctgt
     1381 ggacctacag cctggacgag gtgtggaccg gcctgcagtc cgcgttcgca gacctgatcg
     1441 ccgacgtgca cgaccgctac ggcgtgcgcc ccgacacgtt cggcgccatc ggcgtctccg
     1501 cgatgatgca cggctacctg gccttcgatg cggccggcga tctgctcgcc ccgttccgca
     1561 cctggcgcaa caccaacacg ggtccggccg cagccgagct caccgagctg ctgggcgtga
     1621 acatcccgct gcgctggtcg gtcgcgcacc tgcggcaggc gcagctcgac gccgagccgc
     1681 acgccggcga ggtgcgcttc ctcaccacac tcgccggcta cgtgcactgg cggctcaccg
     1741 ggcgccgggt gctgggcatc ggcgacgcct cgggcatgtt cccgatcgac tccgccgtgc
     1801 gcgactacga ccagaagatg atcgcccgct acgacgagcg cgccggcagc
//
LOCUS       DATMSX010000351         4469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_109694,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000351 DATMSX010000000
VERSION     DATMSX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109694"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4469)
                     /locus_tag="VN041_10930"
     CDS             complement(<1..>4469)
                     /locus_tag="VN041_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWU29586.1"
                     /translation="TPTPGATSPAETPAPEQTPQQTPAPDAAIARISGTSGNAGEHWP
                     LAITEINGDNEGADTYEFLEVVNTTDQPIDLDATGIQFRYHTSQWNTGTVQPLIHLDG
                     EDDAPAVIPAGGVAVLWMNYAEGDARRSLTKDQFRTFYGMQDADVPIIRFGPQGGFAN
                     SGNRGFSITDAGGTTLMRAWVPADDGDGSTPWNAQFGVPDQTDSVDARILAHDVTGAT
                     AVPTPGAITTEQVTSRLIKPTDPSSEGPLLQITEVAPDTANVAGSDAYEYIELYNASD
                     APIDFGDYVLSYLSTDNDLTDPKTLSSTLWPAGPGEPTIEPGATMVLWIQNPAVVSAG
                     LTVADFNTAFGSDLTLGESILTIQSGGMANGGSRGLQLMTKSGHVISRAFYFDDAQTT
                     SSTAIQYAWNPGDPASAWKPADPSGVTQTMLGLAAPTPGAVTEAQVAPTSVAYPAPGS
                     APKIVDLTGGSELPDSGNVELGFDITDDVLVRRVTLTLKDSLGATDVRDLTFGSGDRY
                     QYAIPAVDVYGKRWIEYTVTAGDGSQLTTLGPVRIALDGSPADPVRLNVADGQFVNAQ
                     TPLVGTTDGDPGTLQVGIDGEPAGETVPSLERAPKFAIDATSTDSFFRNGIKLGDNVL
                     KIFDEGYYDRIVTVDADVPVEEVVRGEKLTLGVYAGTKAWPEPNLDENNDDFTMMNPR
                     LALPDGRVLRPTTCAGAGEGKELVERACPADAAERIALSDANLVYILFTFTLPDDAFD
                     SVSTTWDTTAVADGEHVITASEGDRTATRTVIVDNTAPRIESTVTDGSQYRGDFTIDA
                     SVTDEGSGFKSVSATLDEQDITLPLQTSSLTLTPGDHTAVITASDRVGNISTSTVAFS
                     TADEKPHTTLISPDDGAKIDADQVDLVASPGSNVDDRLELCFAEGYRFVPTDAQVHVA
                     SGTTTDSRSTDRDDARALSADELARITRMDGIEVAETSGTALPYQLFTVDVPANAKDG
                     SDVRVAWEGSANADAKVLLYVQTAKGDWNEVDRYVTSGGAPTSFTLDAQVPVTGNARD
                     GKIIFLVQHSEGWAATGRSGRDSQTTPYNPGATPRDQYDFTLAWESDTQYYNSNEGKR
                     AGTGGTDAWYRHQLNINRFLIDRRDELNLQYVAHTGDIVDNQDQDYQWRNADDAYRML
                     DEAGMPYGVLAGNHDVGHFEGNYGPYSSYFGEKRFAQNPWYGGSYKDNRGHFDLVSAD
                     GVDLLVLYMGWPDPNDTVSNSEDIAWMNSVIRQYPERKVMINLHEFMLTTGGLGPFPQ
                     RIYDEVVAPNSNVIAVGSGHYHDAYTRLDEFDDDGDGVADRTVTSMLFDYQALPEGGQ
                     GYLRLLHFDNTSGRIIVRTYSPSLDDFDSDEASLNSPAGMQEFEIPYAAGGIRPAQKT
                     LSTDSFRADVLTANRIGCVQDVVSGEKTSVTWKDLTSGAAPGQASARAQLAATSPANR
                     GWYVRTTGPYGGVEYTEVRTFTATGASTEPTDPGEGGNTPGGGNGGTGPGQGRS"
BASE COUNT          583 a         1431 c         1649 g          806 t
ORIGIN      
        1 gatctaccct ggccggggcc ggtgccgccg tttccaccgc cgggggtgtt cccgccctca
       61 cccggatcgg tgggctcggt cgacgcgccg gtcgccgtga aggtgcgcac ctcggtgtac
      121 tccacaccgc cgtacggacc cgtcgtgcgc acgtaccagc cgcggttcgc cggggaggtg
      181 gccgcgagct gcgcacgagc ggatgcctga ccgggagccg cccccgaggt caggtccttc
      241 caggtgaccg acgtcttctc gccgctcacg acgtcctgca cgcagccgat ccggttcgcg
      301 gtgagcacgt cggcgcggaa cgagtcggtc gacagcgtct tctgcgccgg tctgatgccc
      361 ccggccgcat acgggatctc gaactcctgc atccccgcgg ggctgttcag cgacgcctcg
      421 tcggagtcga agtcgtccag cgagggcgag tacgtgcgca cgatgatgcg accgctcgtg
      481 ttgtcgaagt gcagcaggcg caggtagccc tggccgccct ccggcagcgc ctggtagtcg
      541 aacagcatcg aggtcacggt gcggtcggcg acgccgtctc cgtcgtcgtc gaactcgtcc
      601 agccgcgtgt aggcgtcgtg gtagtggccg gatccgacgg cgatcacgtt ggagttgggt
      661 gccacgacct cgtcgtagat ccgctgcggg aacggcccga gcccgccggt ggtgagcatg
      721 aactcgtgca ggttgatcat gaccttgcgc tcggggtact gccggatcac cgagttcatc
      781 caggcgatgt cctcggagtt cgacacggtg tcgttggggt cgggccagcc catgtacagc
      841 acgagcaggt cgacgccgtc ggcgctcacc agatcgaagt ggccgcggtt gtccttgtac
      901 gagccgccgt accaggggtt ctgcgcgaac cgcttctcac cgaagtacga gctgtacggg
      961 ccgtagttcc cctcgaagtg accgacgtcg tggttgcccg ccagcacgcc gtaaggcatg
     1021 ccggcctcgt ccagcatccg gtatgcgtcg tcggcgttgc gccactggta gtcctggtcc
     1081 tggttgtcga cgatgtcgcc ggtgtgggcc acgtactgca ggttcagctc gtcgcggcga
     1141 tcgatcagga accggttgat gttcagctgg tgccggtacc aggcatccgt cccgccggtt
     1201 ccggcacgct tgccctcatt gctgttgtag tactgcgtgt ccgactccca ggccagcgtg
     1261 aagtcgtact gatcgcgcgg ggtggcaccc gggttgtagg gcgtcgtctg ggagtcgcgg
     1321 ccactgcggc cggtcgccgc ccagccctcg gagtgctgca cgaggaagat gatcttcccg
     1381 tccctggcgt tgccggtgac gggaacctgc gcgtcgaggg tgaacgacgt gggggcgcca
     1441 ccggacgtga cgtaccggtc gacctcgttc cagtccccct tcgccgtctg cacgtacagc
     1501 agcaccttgg cgtcggcgtt cgcggagccc tcccaggcga cgcgcacgtc ggagccgtcc
     1561 ttcgcgttcg ccggaacgtc gacggtgaac agctgatacg gcagcgccgt ccctgaggtc
     1621 tcggcgacct cgatgccgtc catgcgggtg atccgggcca gttcgtcggc gctcagcgcc
     1681 ctggcgtcgt cgcggtcggt ggagcggctg tcggtcgtgg tgccggatgc gacgtgcacc
     1741 tgggcatccg tcggcacgaa tcggtagccc tccgcgaagc agagctccag ccggtcgtcg
     1801 acgttcgagc cggggctcgc gacgaggtcg acctggtcgg cgtcgatctt cgcgccgtcg
     1861 tccgggctga tcagggtggt gtgcggcttc tcgtcggccg tcgagaacgc gacggtgctg
     1921 gtggagatgt tgccgaccct gtcgctcgcg gtgatgaccg cggtgtggtc acccggggtc
     1981 agggtgagcg acgaggtctg cagcgggagg gtgatgtcct gctcgtccag cgtcgcactg
     2041 acgctcttga agcccgaccc ctcatcggtg acggaggcgt cgatcgtgaa gtcaccgcgg
     2101 tactggctgc cgtcggtgac ggtgctctcg atgcgcgggg cggtgttgtc gacgatcacg
     2161 gtgcgcgtcg cggtgcgatc gccctcggat gccgtgatca cgtgctcgcc gtcggcgaca
     2221 gccgtggtgt cccaggtggt ggacaccgag tcgaacgcgt cgtccgggag cgtgaaggtg
     2281 aagaggatgt agaccaggtt ggcgtcgctg agggcgatcc gctccgcggc atccgccggg
     2341 caggcccgct cgacgagctc cttgccctcg cccgcaccgg cgcacgtggt cggacgcagc
     2401 acccggccgt ccggcagtgc caggcgcggg ttcatcatcg tgaagtcgtc gttgttctcg
     2461 tcgaggttcg gctcgggcca ggccttggtg ccggcgtaca cgccgagcgt gagcttctcg
     2521 ccgcgcacga cctcctcgac cgggacatcg gcgtcgaccg tcacgatgcg gtcgtagtag
     2581 ccctcgtcga agatcttcag cacgttgtca ccgagcttga tcccgttgcg gaagaaggag
     2641 tccgtactgg tcgcgtcgat cgcgaacttc ggcgccctct ccagcgacgg gacggtctcg
     2701 cccgcgggct ctccgtcgat gccgacctgc agtgtgcccg ggtcgccgtc ggtggtgccg
     2761 acgagcgggg tctgcgcgtt gacgaactgc ccgtcggcga cgttgaggcg aacggggtcg
     2821 gccggtgagc cgtccagcgc gatccgcacc ggcccgaggg tggtgagctg gctgccgtcg
     2881 cccgcggtga ccgtgtactc gatccagcgc tttccgtaga cgtcgaccgc ggggatcgcg
     2941 tactggtacc ggtcgccgga gccgaaggtc aggtcgcgca catcggtcgc gcccaggctg
     3001 tctttcagcg tgagcgtcac gcgacgcacg agcacgtcgt ctgtgatgtc gaagccgagc
     3061 tcgacgttcc cggagtcggg cagctcggat ccgccggtca ggtcgacgat cttcggtgcg
     3121 gagccggggg ccgggtaggc gacggacgtc ggggcgacct gcgcctccgt gaccgcgccg
     3181 ggcgtgggcg cggcgaggcc gagcatcgtc tgcgtgacgc cggacggatc ggcgggcttc
     3241 catgcggacg cggggtcgcc ggggttccag gcgtactgga tggccgtgct ggacgtggtc
     3301 tgcgcgtcat cgaagtagaa ggcgcgggag atcacgtgcc ccgacttcgt catcagctgc
     3361 agaccgcggg atccgccgtt ggccatgccg ccggactgga tcgtcaggat gctctcgccg
     3421 agagtcaggt cgctgccgaa cgccgtgttg aagtcggcga cggtgagacc tgccgacacg
     3481 acggcaggat tctggatcca cagcaccatc gtcgcgccgg gttcgatcgt cggctcaccg
     3541 gggccggcgg gccacagggt ggacgagagc gtcttcgggt cggtgaggtc gttgtcggtg
     3601 ctgagatagc tcagcacgta gtcgccgaag tcgatcggcg catccgaggc gttgtagagc
     3661 tcgatgtact cgtacgcgtc ggagccggcc acgttcgcgg tgtcgggcgc gacctcggtg
     3721 atctgcagca gcggcccttc ggatgagggg tcggtcggct tgatcagccg actggtgacc
     3781 tgctcggtcg tgatggcgcc gggggtgggc accgcggtcg ccccggtcac gtcgtgggcg
     3841 aggatgcggg cgtcgacgct gtcggtctgg tccggaactc cgaactgggc gttccacggc
     3901 gtggagccgt cgccgtcgtc ggcgggcacc caggcccgca tgagcgtcgt gccgccggca
     3961 tccgtgatgc tgaagccgcg gttgccgctg ttggcgaatc cgccctgcgg gccgaagcgg
     4021 atgatcggca cgtcggcgtc ctgcatgccg tagaaggtgc ggaactggtc cttggtcagg
     4081 ctgcggcgcg cgtcgccctc ggcgtagttc atccagagca cggccacgcc gccggcgggg
     4141 atgaccgcgg gtgcgtcgtc ctcgccgtcg aggtggatga ggggctgcac ggtgcccgtg
     4201 ttccactgcg acgtgtggta ccggaactgg atgccggtgg cgtcgaggtc gatgggctgg
     4261 tcggtggtgt tgacgacttc gaggaactcg taggtgtcgg cgccctcgtt gtcaccgttg
     4321 atctcggtga tggccagcgg ccagtgctca cccgcgttgc ctgaggtgcc ggagatccgg
     4381 gcgatcgcgg catcgggcgc cggcgtctgc tgcggcgtct gctcgggtgc cggagtctca
     4441 gccggcgacg tcgcgccggg ggtcggggt
//
LOCUS       DATMSX010000352        14252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_185302,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000352 DATMSX010000000
VERSION     DATMSX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185302"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1202
                     /locus_tag="VN041_10935"
     CDS             27..1202
                     /locus_tag="VN041_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU29587.1"
                     /translation="MTSEPVRVLAHRGAAGILRSELTRRGFLATALAAGGAVLFTACA
                     PGQVGTAAQANGGPLEDRLSIYTWSDYDEPELLSAFTEKKGPKIVVDAFNSNEELIAK
                     LVAARGTSGYDIVVPSGLFVMPMAENGLLEKLNLDLIPNFATMNPNFIHRSFDPDNAY
                     SICKAWGTTGFVYDKRVINRELTTWADFIDAAQNEASGKTSVLDDPAPLCGIYHWSHG
                     VDWNTTRKKDLDACEKFLVTQLAPHISIFDSAPGGQVIPQASAALVQSYSGDARLGMD
                     ESDDPERWQWVLGAPATELWMDNWAIAKGAPHPEAAHAFIDYIIAPDIQVAQVDYIGY
                     DTGVQGTQQKAEEAELDRLDMVYFSDEQVKTMHEQKFTSAQERIVEIWNKVKVAAGA"
     gene            1195..2085
                     /locus_tag="VN041_10940"
     CDS             1195..2085
                     /locus_tag="VN041_10940"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29588.1"
                     /translation="MRKVRMPFALALPAWVWLAVFFVVPIGVVVWYSFGYKPSIFSTN
                     SNDRLSFDRYVEGLSPTFFNTFQNTFWVGVIGTLLCLIIGAPVAYWIAVKAAPSKRGM
                     LLALVMVPFWTNFLVRTIGWQVILAPEGWLSGALQFIGVIPGPLDILNSRGAVLLGVV
                     YNYLPLMILPLYVAFDRVSGPLREASKDLGANSLVTFLRVTVPIAQPGIIAGVLLVYI
                     PLMGDYITATVLGGAKGNMVGQMVASQFQTAQNWALGSAMAVLLIITIAVSIGVAALL
                     LWLVTMPLRRRYALRLGEGS"
     gene            2082..2909
                     /locus_tag="VN041_10945"
     CDS             2082..2909
                     /locus_tag="VN041_10945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29589.1"
                     /translation="MSRWLLPVWSTLVFVFLFLPIIVIIVYSFNVGRLLVSWDHFGFD
                     SFAAIVTKPAIRDAVLVSVRTGLLAAVIATVLGTLAGIAMARNPGKWVVWFLGMLLLI
                     SVTPEIVDAVALLPWMVFLGQDAGIGIFNDGTVRLIIGQSLFSIAIVSYIVRARLVGL
                     DASLEEASADLYAPPFRTFVKVTLPLAMPAVLAGFLLSFTLGLDNTIVSAFVQVSGTT
                     PWPVYVLSALRSGLRPEIAAVSTIMLVLTLASLALVALVLKRAGDSATDIARTMAGG"
     gene            complement(2970..4130)
                     /locus_tag="VN041_10950"
     CDS             complement(2970..4130)
                     /locus_tag="VN041_10950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWU29590.1"
                     /translation="MSTFDAAAFLPDDLLERIRERAAVHDRENTFPQQDLDELKAAGY
                     LSILVPAELGGAGLGLEQAALLQQRLATASPATALAINMHLVWTGVAKVFSDRGVPGL
                     EFVQRGAVAGEVFAFGISEGGNDLVLFGSDTDAAPQPDGGYAFTGTKIFTSLAPVWTK
                     LGLHGLDTTGEDAPKLVFAFIDRTDAVVTGDDWDTLGMRGTQSRTTRLNGAIAAPEHV
                     VRRIDPGPNPDPIVFGIFSVFEILLASVYTGIARRALDLAVETAQKRRSKKSGTTYSQ
                     DPDIRWRVAGMGLAYDALPPQISALARDVDQLADHGPRWFTLLSGVKHRAVVMAKQVA
                     DEAMLVAGGGSYFSSNELSRLYRDVLAGHFHPSDPESAHATAATAWLGPLED"
     gene            complement(4168..5382)
                     /locus_tag="VN041_10955"
     CDS             complement(4168..5382)
                     /locus_tag="VN041_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29591.1"
                     /translation="MAGYRDLLRTPGVGRMIAAQLVARFPNGMMSLAILMHVEQQSGS
                     YGAAGLVLAAASVGQAIAGPVTSRWMGAWGMRRVLTLTLSVCVAAVLCLALLPLSIAG
                     YMAFAMLAGLSTPPVQSAVRTIYPKLVNAGQLTPLFSLDASLQEIIWIVAPVLITIVS
                     TQVGTVQGLLLVAIILAGGGTWFILSPEVGRVRIPRSRRAFGRVVLKPPVLLATVIGF
                     LLIGACSAVEVGVVATFGHGGLEAGLVLAVFSVGSLAGGLAFGHIPIGPWAMARRLLI
                     VTVGLGLTMVTLNVFWIGGTLLLAGIGIAPALAVLFAITTASVKFSETAEAFGWAGTG
                     QLIGAAAGSAVAGILIDAGGPSGAYLAAALFALAGLIVSVVFVRAFPDLRHRDPSPYP
                     DTETLSIPTVRP"
     gene            5750..5983
                     /gene="nrdH"
                     /locus_tag="VN041_10960"
     CDS             5750..5983
                     /gene="nrdH"
                     /locus_tag="VN041_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HWU29592.1"
                     /translation="MSITVYTKPSCVQCNATYRALDAKGIEYEIHDLSEDATALEQVK
                     ALGYMQAPVVVTDEDHWSGFRPDKIDELAARLA"
     gene            5996..6445
                     /gene="nrdI"
                     /locus_tag="VN041_10965"
     CDS             5996..6445
                     /gene="nrdI"
                     /locus_tag="VN041_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HWU29593.1"
                     /translation="MSAVATAAPLLVYFSSVSGNTARFIEKLGLPARRIPLHRNDEQI
                     VVDEPYVLVTPTYGGGQGRGEEQGAVPKQVIRFLNDEHNRSLIRGVISAGNTNFGESF
                     CLAGEIVSRKCHVPHLYRLEVFGTPDDVDRVSDGLERWWTAHSKSSQ"
     gene            6412..8586
                     /gene="nrdE"
                     /locus_tag="VN041_10970"
     CDS             6412..8586
                     /gene="nrdE"
                     /locus_tag="VN041_10970"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HWU29594.1"
                     /translation="MVDSALEEQSVTEQVEFKVNPAYEGLDYHALNAMLNLYDANGKI
                     QFDADKRAAREYFLQHVNQNTVFFHSLKERLDYLVEKEYYEAAVIDQYSFEFIQKLND
                     FAYSKKFRFETFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMTALALAEGDEQVAIDL
                     VDEIISGRFQPATPTFLNAGKAQRGELVSCFLLRIEDNMESIARGINSSLQLSKRGGG
                     VALLLSNIRESGAPIKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLSAHHP
                     DIMRFLDTKRENADEKIRIKTLSLGVVVPDITFELAKKGEDMYLFSPYDVEKVYGVPF
                     GDISVTEKYYEMVDDARIKKTKINAREFFQTIAEIQFESGYPYIMFEDTVNKANPIKG
                     RINMSNLCSEILQVNTPTTYSTDLSYDQIGKDISCNLGSMNIALAMDADDLGKTVETA
                     IRALTAVSTQSHISSVRSIEDGNDRSHAIGLGQMNLHGYLAREHVFYGSEEGIDFTNI
                     YFYTVLFHALRASNRLAIERGEAFDGFEDSTYASGAFFDKYIERAWQPETEKVKELFA
                     GKFVPTQQDWVELRDSIQEHGIYNQNLQAVPPTGSISYINNSTSSIHPIASKIEIRKE
                     GKLGRVYYPAPFMTNDNLEYYQDAYEIGYEKVIDTYAAATQHVDQGLSLTLFFKDTAT
                     TRDINKAQIYAWRKGIKTIYYIRLRQMALEGTDMTECVSCML"
     gene            8630..9610
                     /gene="nrdF"
                     /locus_tag="VN041_10975"
     CDS             8630..9610
                     /gene="nrdF"
                     /locus_tag="VN041_10975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HWU29595.1"
                     /translation="MTPSPKLQLVSHVDAINWNRIEDDKDLEVWNRLTGNFWLPEKVP
                     LSNDVQSWNTLTADEQLLTMRVFTGLTLLDTIQGTVGAVSLIPDAITPHEEAVYTNIA
                     FMESVHAKSYSSIFSTLASTKEIDEAFRWSTENQNLQKKAQIIMEYYRGDDPLKRKVA
                     STLLESFLFYSGFYLPIYWSSKAKLTNTADLIRLIIRDEAVHGYYIGYKYQRGLETET
                     PERRDELKDYTFSLLYELYDNEVQYTQDLYDGVGLTEDVKKFLHYNANKALMNLGYEA
                     MFPSTVTNVNPAILSALSPNADENHDFFSGSGSSYVIGKAEATEDDDWDF"
     gene            complement(9671..10066)
                     /locus_tag="VN041_10980"
     CDS             complement(9671..10066)
                     /locus_tag="VN041_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU29596.1"
                     /translation="MCASLSRLPRERRRTWTDVDRAKEFYTMIGFNPDHDHQVSDELR
                     FVQMTPPGSACSIAFGTGTGMGSGLEPGQQNSIQIVVPDADEALSHLHGLGVEASGVD
                     EQPWGRFVTFADPDGNTWTLQQIPDYGAQ"
     gene            10149..10682
                     /locus_tag="VN041_10985"
     CDS             10149..10682
                     /locus_tag="VN041_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29597.1"
                     /translation="MDTIRLRLWTVDDIGLLRAANTPEMTAHLNGPETEEQIIDRNER
                     YLRLGAAGEARNFVIVDAQDVPLGSIAYWRADWRGEPVLEAGWFVLPEAQGRGVAARA
                     LALVIDDAREHRGERRLLTAFPAADNAASNGVCRRGGFTQTGTFTEEFRGADLTMNEW
                     ALALDGSGADTFPTALR"
     gene            complement(10758..12263)
                     /locus_tag="VN041_10990"
     CDS             complement(10758..12263)
                     /locus_tag="VN041_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29598.1"
                     /translation="MNRSSAGAVELRDLIDTPDLAGLSVLHLVSGRSPVRDLALVADF
                     EDIGSVGPDTVVLLAPGGARGGWMISAALRYAWERRACALIVPEQPFTASVIELAKRL
                     EVSLLTTRRDMTRLAIDAAIRIGAARAEAMVRVRAVVDRLATVSGPAQAVDMLSDELS
                     GAQVWIESSGARTFASPMPREADADPRPMSVVRARLAPRTEEQDLVLARVPAHAVVFG
                     EQLLAGAAPSLRALLLEQRLNATRTSLPAISITALTGFAPIATFMEPSEPEDEQRARL
                     PLDGAFLAVCLLTEEPDRLGALVHQLWQLAFPEAPLARFEGGWIGFLPVGDTTEQRAE
                     TLARVRSGFVRAAGVPITGGCSRVHRSARSARHAVREAWLAARLADAGGATPEAPSSD
                     AFVEFDHAPLHLLKRVVPADLADQLVEALFPQLIADPSAPRLIEAVLGYLSANGSIST
                     AAARLGLHRNTVQTRLQRAAALGVDLTEQANVLPVHLLFAAMHRADPRVDV"
     gene            complement(12260..13339)
                     /locus_tag="VN041_10995"
     CDS             complement(12260..13339)
                     /locus_tag="VN041_10995"
                     /inference="COORDINATES: protein motif:HMM:NF044937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWU29599.1"
                     /translation="MRLSAEDVRHLRTGALFLACAADPEWCEEQQDRILARMEGGVAP
                     ELLGVEEFDEDALVAAVGFVNNGLPLSELRPAGDEFVSSVELLERELGRRIEGLFPLA
                     AANVNSMVPLLTGMQLGRPVVDADPMGRVFPLLFQSVFTLAGLPAGPLAATGPVGESA
                     FLRVRDPRRAERLVRALAEEFGGWSATALYPMTARDLARTGVLGSVSRMVRIGRILDS
                     DARVSAKHEQLRRSEGVRRIIRARVADVAGLSRPAPAGEPDHPSSVVLIEESQGRFVQ
                     LEIQNELLLLMVDGTPEAVIPDIITMLHPDDGRVASLDDLWVGNTLDLVVLPAAGQWY
                     TPEGRRLAAPETLHSLLPDRGRAPR"
     gene            complement(13323..>14252)
                     /locus_tag="VN041_11000"
     CDS             complement(13323..>14252)
                     /locus_tag="VN041_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29600.1"
                     /translation="SAAAIPPGSRVVITSVGALVNPAHELAAGEILLEHGGPLSVVFS
                     HAFASNSFATREHTAILNDALVPDAEALATALALVAGGRFPDARLSVTRNDGGRVPLS
                     RLPAEPIHSAFSGAPVDFLGAAALCGLDDGDLVLASDDGLALGTLRAGAPAVIPRLRW
                     KGARLSTQAAHLVPATEILLHGRTTIPHLVRVAGAPLPGWLDGGEVVETDHDLRALGA
                     ACAPLSDWALGTVGAVNAAEMAHELQAAEARVRARLAAFGALPSSVRILESQLVATTY
                     EHARVVSVRVRGVAGDPVGGLLAGRKVRHAAQR"
BASE COUNT         2440 a         4759 c         4887 g         2166 t
ORIGIN      
        1 gtgatcgcgc tgcccaaggc cgtccgatga cctccgaacc ggtccgggtg ctcgcgcacc
       61 gcggtgcggc aggcatcctg cgcagtgaac tgacccgccg gggattcctc gccacggccc
      121 tcgcggccgg cggagccgtg ctgttcaccg cctgcgcacc cggccaggtc gggaccgcgg
      181 cccaggcgaa cggtggcccg ctcgaggacc ggctgtcgat ctacacctgg agcgactacg
      241 acgagcccga actgctctcc gcgttcaccg agaagaaggg gccgaagatc gtcgtcgacg
      301 cgttcaactc caatgaggag ctgatcgcga agctggtggc ggcgcgcggg acctctgggt
      361 acgacatcgt cgtccccagc gggctgttcg tcatgccgat ggccgagaac ggtctgctcg
      421 agaagctgaa cctcgacctc atcccgaact tcgccacgat gaacccgaac ttcatccatc
      481 gctccttcga ccccgacaac gcgtactcga tctgcaaggc atggggcacg accggcttcg
      541 tctacgacaa gcgcgtcatc aaccgcgagc tcacgacgtg ggccgacttc atcgacgccg
      601 ctcagaacga ggcatccggc aagacctcgg tcctcgacga cccggcgccg ctgtgcggca
      661 tctaccactg gtcgcacggc gtcgactgga acaccacacg caagaaggac ctggacgcct
      721 gcgagaagtt cctggtgacg cagctcgcgc cgcacatctc gatcttcgac tccgcaccgg
      781 gcgggcaggt gatcccgcag gcgtctgcgg cgctcgtgca gtcgtacagc ggcgacgcca
      841 ggctcggcat ggacgagtcc gacgaccccg aacggtggca gtgggtgctc ggcgcaccgg
      901 cgacggagct gtggatggac aactgggcga tcgccaaggg agccccgcac ccggaggcgg
      961 cgcacgcgtt catcgactac atcatcgccc ccgacatcca ggtggcgcag gtcgactaca
     1021 tcggctacga cacgggcgtg cagggcacgc agcagaaggc cgaggaggcc gagctggacc
     1081 ggctcgacat ggtgtacttc agcgacgagc aggtgaagac gatgcacgag cagaagttca
     1141 ccagcgcaca ggagcgcatc gtggagatct ggaacaaggt gaaggtggcc gcaggtgcgt
     1201 aaggtcagga tgccgttcgc gctggcgctg ccggcatggg tgtggctcgc cgtgttcttc
     1261 gtcgtgccga tcggagtcgt cgtctggtac agcttcggct acaagccgag catcttctcg
     1321 acgaactcca atgaccggct ctccttcgac cgctacgtcg aggggctctc gccgaccttc
     1381 ttcaacacgt tccagaacac cttctgggtc ggtgtcatcg gcaccctgct gtgcctgatc
     1441 atcggggcgc cggtggcgta ctggatcgcc gttaaggcgg cgccctcgaa gcgcgggatg
     1501 ctgctggcgc tggtgatggt gccgttctgg acgaacttcc tggtgcgcac gatcggctgg
     1561 caggtgatcc tggcgcccga gggatggttg tccggcgcac tgcagttcat cggcgtcatc
     1621 cccggtccgc tcgacatcct caactctcgg ggagccgtgc tgctgggcgt cgtctacaac
     1681 tacctgccgc tgatgatcct gccgctgtac gtcgccttcg accgtgtcag cggtcctctg
     1741 cgcgaggcga gcaaggacct cggggcgaac agtctcgtca ccttcctgcg cgtgacggtg
     1801 ccgatcgcgc agccgggaat catcgccggt gtcctgctgg tgtacatccc gctgatgggc
     1861 gactacatca ccgccacggt gctgggcgga gcgaagggca acatggtcgg ccagatggtg
     1921 gccagccagt tccagaccgc gcagaactgg gcgctgggat cggcgatggc cgtgctgctc
     1981 atcatcacca tcgccgtctc gatcggcgtc gccgcgctgc tgctgtggct ggtcacgatg
     2041 ccgttgagac ggcgatacgc cctgaggttg ggagagggat catgagccgc tggcttcttc
     2101 cggtgtggtc gacgctggtc ttcgtcttcc tgttcctgcc gatcatcgtg atcatcgtgt
     2161 attcgttcaa cgtcgggcga ctgctcgtct cgtgggatca cttcggcttc gactccttcg
     2221 ccgcgatcgt caccaagccg gcgatcaggg atgccgtgct ggtgtcggtg cgcacgggtc
     2281 tgctcgccgc ggtcatcgcg acggtgctcg gcacactcgc cggcatcgcg atggcgcgga
     2341 accccggcaa atgggtggtg tggttcctgg gcatgctgct gctgatctcg gtgacacccg
     2401 agatcgtgga tgccgtcgcg ctgctgccgt ggatggtgtt cctcggccag gacgccggga
     2461 tcgggatctt caacgacggc accgtgcgac tgatcatcgg gcagtcgctg ttctccatcg
     2521 cgatcgtgtc gtacatcgtg cgggcgcgac tggtcggact ggatgccagt ctggaggagg
     2581 catccgccga cctctacgcg ccgccgttcc gcaccttcgt gaaggtcacg ctgccgctgg
     2641 ccatgccggc ggtgctggcc ggcttcctgc tctcgttcac gctcggactg gacaacacga
     2701 tcgtctcggc gttcgtgcag gtgtcgggca cgacgccgtg gccggtgtac gtgctcagcg
     2761 ccctgcgcag cggtctgcga ccggagatcg ccgccgtctc caccatcatg ctcgtgctga
     2821 cgctcgcgtc gctggcgctc gtggcgctcg tgctcaagcg ggcgggggac tcggccaccg
     2881 acatcgcccg cacgatggcg ggcggctgag tcgccgagcg gcggcaaccc tccggttctc
     2941 ggatgctcag ggtcagcgga tcgagggtct cagtcctcga gcggtccgag ccaggcggtc
     3001 gcggctgtgg cgtgcgcgga ctccgggtcg gaagggtgga agtgacctgc cagcacatcg
     3061 cggtacagcc gcgagagctc gttggatgag aagtacgatc cgccgccggc caccagcatc
     3121 gcctcgtcgg cgacctgctt ggccatgacg accgcgcgat gcttcacgcc tgagagcaga
     3181 gtgaaccagc gcggtccgtg atcggcgagc tggtcgacgt ctcgggccag cgcggagatc
     3241 tgcggcggca gcgcgtcgta tgccaggccc atgcccgcga cccgccagcg gatgtcggga
     3301 tcctggctgt aggtcgtgcc ggacttcttc gagcggcgct tctgcgcggt ctcgacggca
     3361 agatccagcg cgcggcgggc gatgccggtg tacaccgagg cgagcaggat ctcgaacacg
     3421 ctgaagatgc cgaacacgat cgggtcgggg ttcgggccgg ggtcgatgcg gcgcaccacg
     3481 tgctcggggg cggcgatcgc gccgttcagg cgggtcgtgc ggctctgcgt tccgcgcatg
     3541 ccgagcgtgt cccagtcgtc gccggtgacc acggcatccg tgcggtcgat gaacgcgaag
     3601 acgagtttcg gggcgtcctc accggtcgtg tccaggccat gcaggccgag cttcgtccag
     3661 accggcgcga gcgaggtgaa gatcttggtg cccgtgaagg cgtagccgcc gtcgggctga
     3721 ggtgcggcat ccgtgtcgct gccgaacagc accagatcgt tgccgccctc gctgatcccg
     3781 aaggcgaaga cctcgcctgc gacggcgccg cgctgcacga actcgagccc gggcacgccg
     3841 cggtcgctga acaccttcgc gacgccggtc cacaccaggt gcatgttgat ggcgagcgcc
     3901 gtggcggggg atgcggtcgc caggcgctgc tgcagcaggg ccgcctgctc gagacccagg
     3961 cccgctccgc ccagctcggc gggcacgagg atcgagaggt atccggcagc cttcagctcg
     4021 tcgaggtcct gctgcgggaa cgtgttctcg cggtcgtgca cggcggcgcg ctcgcggatg
     4081 cgctccagca ggtcgtcggg aaggaaagca gcggcgtcga aggtgctcac gcgcccagcc
     4141 tatgccgcga ggatcgtgcg cggaccgtca ggggcggacg gtcgggatgg agagcgtctc
     4201 ggtgtcgggg tagggactgg gatcgcggtg ccgcaggtcg gggaaggctc ggacgaagac
     4261 caccgagacg atcagcccgg cgagggcgaa cagcgccgcg gcgaggtacg cgccgctcgg
     4321 accgcccgcg tcgatcagga tgcccgcgac cgcggagccc gccgcggcgc cgatcaactg
     4381 cccggtgccc gcccagccga acgcctcggc ggtctcgctg aacttcacgc tcgcggtggt
     4441 gatcgcgaac agcacggcga gggccggggc gatgccgatg cccgccagca gcagagtgcc
     4501 gccgatccag aacacattga gcgtgaccat ggtcaggccc agtccgaccg tgacgatgag
     4561 cagccgcctg gccatcgccc aggggccgat ggggatgtgg ccgaacgcga gtccgccggc
     4621 caggctgccg accgagaaca ccgccagcac gagcccggcc tccagcccgc cgtgcccgaa
     4681 ggtggcgacg acgcccacct cgaccgcgga gcatgctccg atcaggagga agccgatgac
     4741 ggtcgccagc agcaccggcg gcttgaggac gacacgtccg aacgcgcgcc tgctgcgcgg
     4801 gatgcgcacg cgcccgacct cgggggagag gatgaaccag gtgccgccgc cggcgaggat
     4861 gatcgcgacc agcagcaggc cctgcacggt gccgacctgc gtggagacga tcgtgatcag
     4921 cacgggggcg acgatccaga ttatctcctg cagcgaggcg tccagcgaga acagcggcgt
     4981 gagctggccc gcgttgacga gcttcgggta gatggtgcgc accgccgact gcacgggagg
     5041 agtggagagc ccggcgagca tcgcgaaggc catgtagccg gcgatgctca gcggcagcag
     5101 agccaggcag agcacggccg cgacgcacac ggacagggtg agggtgagca cacggcgcat
     5161 gccccaggcg cccatccagc gactcgtcac cgggcccgcg atggcctgac cgacggatgc
     5221 cgctgcgagc acgaggcccg cggcgccgta ggacccggac tgctgctcga cgtgcatcag
     5281 gatcgccagg ctcatcatgc cgttggggaa gcgcgccacc agctgcgccg cgatcatgcg
     5341 gccgactccc ggcgtgcgaa gaaggtcccg atatcccgcc accagagaac cctatccggg
     5401 gtgcgcggcg gcgcgcgagg tgtcggatgc cgcgacacgc cgcgacacgc cgaagagcgt
     5461 tctccacagg aaatcggatg tcggtggtgc ggcgtagcgt gcggcctgtg gatggatccg
     5521 gcgaccggcc ccggaagccg cgagaactcg cggttcgcgg gccgttccga ggggctcccg
     5581 gctgtggaag aaaccgtgga gaagctgtgt tgaacatggg gagagcggtg gaaaactaca
     5641 cggatgtaac tactatccct agtggtcggt cgcaatgtcc gtccctatat gtagtattga
     5701 agtcccggtg gggggtctgc cggagaacag ctgagattga ggggaattca tgtcgatcac
     5761 ggtctacacc aagccttcct gcgtgcagtg caacgccacc tatcgcgcgc tcgatgcgaa
     5821 gggcatcgag tacgagatcc acgacctctc cgaggacgcc acggcgctcg agcaggtcaa
     5881 ggcgctcggc tacatgcagg cgccggtcgt cgtcaccgac gaggaccact ggtcgggctt
     5941 ccgtcccgac aagatcgacg agctcgccgc ccgtctggcg tgagtgtggc cggccatgag
     6001 cgcagtcgcg accgcagcgc cgctcctggt ctacttctcg agcgtctcgg gtaacaccgc
     6061 tcgcttcatc gagaagctcg ggcttcctgc tcgccgcatc cctctgcacc gaaacgacga
     6121 gcagatcgtc gtcgacgagc cgtacgttct cgtcaccccc acctacgggg gcggtcaggg
     6181 gcgcggcgag gagcagggcg ccgttcccaa gcaggtgatc cggttcctca acgatgagca
     6241 caaccgcagc ctgatccgcg gagtgatctc cgcggggaac accaacttcg gcgagtcgtt
     6301 ctgcctcgcc ggagagatcg tgagccgcaa gtgtcatgtg ccgcacttgt accggctgga
     6361 ggtattcggc acgccggatg atgtcgatcg cgtgagcgac ggattggaac gatggtggac
     6421 agcgcactcg aagagcagtc agtgacggag caggtggagt tcaaggtcaa ccccgcatac
     6481 gaggggctcg actatcacgc gctgaacgcg atgctgaacc tgtacgacgc gaacggcaag
     6541 atccagttcg acgccgacaa gcgcgccgct cgggagtact tcctgcagca cgtgaaccag
     6601 aacaccgtgt tcttccactc gctcaaggag cgcctggact acctggtgga gaaggagtac
     6661 tacgaggccg cggtcatcga ccagtactcg ttcgagttca tccagaagct caacgacttc
     6721 gcctactcga agaagttccg cttcgagacc ttcctcggcg cgttcaagta ctacaccagc
     6781 tacacgctga agaccttcga cggcaagcgc tacctggagc gcttcgagga ccgcgtcgtg
     6841 atgaccgccc tcgcgctcgc cgagggcgac gagcaggtcg cgatcgacct ggtcgacgag
     6901 atcatctccg gccgcttcca gccggccacc cccacgttcc tcaacgcagg aaaggcgcag
     6961 cgcggcgagc tcgtcagctg cttcctgctg cgcatcgagg acaacatgga gtcgatcgcc
     7021 cgtggcatca actcctcgct gcagctgtcc aagcgcggcg gcggcgtggc gctgctgctg
     7081 tcgaacatcc gcgagtcggg tgccccgatc aagcagatcg agaaccagtc ctccggcatc
     7141 atcccggtca tgaagctcct cgaagacagc ttcagctacg ccaaccagct cggcgcgcgt
     7201 cagggcgccg gcgcggtgta cctctcggcg caccaccccg acatcatgcg gttcctcgac
     7261 accaagcgtg agaacgccga cgagaagatc cgcatcaaga cgctgtcgct gggcgtggtc
     7321 gtccccgaca tcaccttcga gctcgccaag aagggcgagg acatgtacct gttctcgccg
     7381 tacgacgtcg agaaggtcta cggcgtgccc ttcggcgaca tctcggtcac cgagaagtac
     7441 tacgagatgg tcgacgacgc gcgcatcaag aagaccaaga tcaacgcgcg tgagttcttc
     7501 cagaccatcg ccgagatcca gttcgagtcg ggctacccgt acatcatgtt cgaggacacg
     7561 gtgaacaagg cgaacccgat caagggccgc atcaacatgt ccaacctgtg cagcgagatc
     7621 ctgcaggtga acaccccgac cacgtacagc accgacctgt cgtacgacca gatcggcaag
     7681 gacatctcct gcaacctcgg ttcgatgaac atcgcgctgg cgatggacgc cgatgacctg
     7741 ggcaagaccg tcgagaccgc gatccgcgct ctcaccgcgg tgagcacgca gagccacatc
     7801 agctcggtgc gctcgatcga ggacggcaac gaccggtcgc acgcgatcgg cctgggccag
     7861 atgaacctgc acggctacct ggcgcgcgag cacgtgttct acgggtcgga agagggcatc
     7921 gacttcacga acatctactt ctacacggtg ctgttccacg cgcttcgcgc atcgaaccgc
     7981 ctggcgatcg agcgcggcga ggccttcgac ggcttcgagg actcgaccta cgcgtcggga
     8041 gcgttcttcg acaagtacat cgagcgcgcc tggcagcccg agaccgagaa ggtcaaggag
     8101 ctgttcgccg gcaagttcgt cccgacccag caggactggg tggagctgcg cgacagcatc
     8161 caggagcacg gcatctacaa ccagaacctg caggccgtgc cgccgacggg atcgatctcc
     8221 tacatcaaca actcgacctc gtcgatccac ccgatcgcct cgaagatcga gatccgcaag
     8281 gagggcaagc tcggtcgcgt gtactacccg gcgccgttca tgacgaacga caacctggag
     8341 tactaccagg acgcgtacga gatcggctac gagaaggtca tcgacacgta cgccgccgcc
     8401 acccagcacg tggaccaggg cctgtcgttg acgctgttct tcaaggacac cgccaccacc
     8461 cgcgacatca acaaggcgca gatctacgca tggcgcaagg gcatcaagac gatctactac
     8521 atccgcctgc gtcagatggc cctcgagggg accgacatga ccgagtgcgt cagctgcatg
     8581 ctgtgatcgc tctctgatca gaatttctcc gagaacagga ctacaggaaa tgactccctc
     8641 tccgaagctt cagctggtca gtcacgtcga tgcgatcaac tggaaccgca tcgaggacga
     8701 caaggacctc gaggtctgga accgtctcac cggcaacttc tggctgcctg agaaggtgcc
     8761 gctgtcgaac gacgtgcagt cgtggaacac gctcaccgcc gacgagcagc tgctcacgat
     8821 gcgcgtgttc accggcctca ccctgctcga cacgatccag ggcacggtcg gcgccgtctc
     8881 gctgatcccc gacgcgatca ccccgcacga ggaggccgtg tacaccaaca tcgccttcat
     8941 ggagtcggtg cacgccaaga gctactcctc gatcttctcg acgctcgcgt cgacgaagga
     9001 gatcgatgag gccttccgct ggtccactga gaaccagaac ctgcagaaga aggcgcagat
     9061 catcatggag tactaccgtg gtgacgaccc gctcaagcgc aaggtcgcct ccacgctgct
     9121 ggagtcgttc ctgttctact cgggcttcta cctgccgatc tactggtcgt ccaaggcgaa
     9181 gctgacgaac acggccgacc tgatccgcct gatcatccgc gacgaggccg tgcacggcta
     9241 ctacatcggg tacaagtacc agcgcggcct cgagaccgag acgccggagc gccgtgacga
     9301 gctgaaggac tacaccttct cgctgctgta cgagctctac gacaacgagg tgcagtacac
     9361 gcaggacctc tacgacggcg tcggcctgac cgaggacgtc aagaagttcc tgcactacaa
     9421 cgccaacaag gccctgatga acctgggcta cgaggcgatg ttcccctcca ccgtcaccaa
     9481 cgtgaacccg gcgatcctgt cggccctgtc gccgaacgcc gacgagaacc acgacttctt
     9541 ctcggggtcg ggctcgtcgt acgtgatcgg caaggccgag gccaccgagg acgacgactg
     9601 ggacttctga gccggatctc gaacgaaggg agggcggatg ctgcacagca tccgccctcc
     9661 cttcgttcgc tcactgcgcc ccgtagtccg ggatctgctg cagcgtccag gtgttgccgt
     9721 cggggtcggc gaaggtgacg aatcggcccc acggctgctc gtcgacgccg gaggcctcga
     9781 caccgagccc gtgcaggtgc gacagagcct cgtcggcatc cggtacgacg atctggatcg
     9841 agttctgctg accgggctcg agcccgctgc ccatgccggt gccggtgccg aaggcgatgg
     9901 agcaggccga tccgggaggg gtcatctgca cgaagcgcag ctcgtcggag acctggtggt
     9961 cgtgatcggg attgaagccg atcatcgtgt agaactcctt cgcgcggtcg acgtccgtcc
    10021 atgtcctgcg cctttctcga gggagacgcg agaggctcgc gcacatgtgc gacggtacgc
    10081 ggggccaccg acatccgggg gagcacctcg cgctcgcgga tgtcggaggc ccgtgagagc
    10141 gtagccgcgt ggacacgata cgactgcgac tgtggacggt ggatgacatc ggcctgctcc
    10201 gcgcggcgaa caccccggag atgacggcgc acctgaacgg cccggagacc gaggagcaga
    10261 tcatcgaccg caacgagcgg tatctgcgac tcggagcggc cggtgaggcg cggaacttcg
    10321 tgatcgtgga cgcccaggac gtcccgctcg gatccatcgc gtactggcgc gccgattggc
    10381 ggggcgagcc cgtgctggag gcgggctggt tcgtgctccc cgaggcgcag ggacgcgggg
    10441 tggcggcgcg ggcgctcgca ctggtcatcg acgatgcgag ggagcatcgc ggtgagcgtc
    10501 gcttgctcac ggcgttcccc gccgccgaca acgccgcctc caacggcgtc tgccggcgcg
    10561 gaggcttcac gcagaccgga accttcaccg aggagttccg cggggcggac ctcaccatga
    10621 acgaatgggc gctcgcgctc gacgggtcgg gagccgacac gttccccacg gcgctgcgct
    10681 gacgcagcgc gggcgtcggt acggccagca atggggatga ggcgccccgg ggccttcgtc
    10741 gtgtccgccg aggcctctca cacgtccacg cgggggtccg cgcgatgcat cgcagcgaag
    10801 agcagatgca cggggagcac gttcgcctgc tcggtgaggt cgacgccgag cgcggcggcg
    10861 cgctgaagcc tggtctgcac ggtgttgcgg tgcagaccga gacgcgcggc ggcggtcgat
    10921 atcgacccgt tcgccgacag gtagccgagc accgcctcga tgaggcgggg agccgaggga
    10981 tcggcgatga gctgcgggaa cagcgcctcg acgagttgat cggcgagatc cgccggcacg
    11041 acccgtttga gcaggtgcag cggtgcgtgg tcgaactcca cgaaggcgtc ggaactgggc
    11101 gcctcgggtg tcgccccgcc ggcatccgcg agacgcgcgg cgagccaggc ctcgcggacc
    11161 gcgtgccttg cgctcctcgc ggaacgatgg actcgtgagc agcctccggt gatcgggacg
    11221 cccgcggcgc gcacgaaccc gcttctcacc cgtgccagcg tctccgcccg ctgctcggtg
    11281 gtatccccga cgggaagaaa gccgatccag ccgccttcga atcgggcgag cggtgcctcg
    11341 ggaaaggcga gctgccagag ctggtgcacg agggcgccca ggcgatcggg ctcctcggtg
    11401 agcaggcaca cggccaggaa tgcgccgtcg agcgggagcc gcgctcgctg ttcgtcctcc
    11461 ggctccgacg gctccatgaa cgtggcgatc ggtgcgaacc cggtcagcgc cgtgatcgag
    11521 atggcgggaa gcgaagtcct ggtggcgttc agtcgctgct cgagaagcag tgccctgagc
    11581 gatggcgcgg cccccgcgag cagctgctcg ccgaagacca ccgcatgtgc gggaaccctg
    11641 gcgagcacca ggtcctgttc ctcggtccgg ggcgcgagcc tggcgcggac gaccgacatg
    11701 ggccgagggt cggcatcggc ctcgcggggc atcggcgacg cgaatgtgcg ggcgccggag
    11761 gactcgatcc acacctgcgc tccgctgagc tcgtccgaca gcatgtcgac ggcctgcgcc
    11821 ggcccggaga ccgtcgccag ccggtcgacg acggcgcgca cgcggaccat cgcctcggcg
    11881 cgcgcggcgc cgatccggat cgccgcatcg atcgcgaggc gggtcatatc gcgccgggtg
    11941 gtgagcagcg agacttccaa gcgcttcgcg agctcgatga ccgacgcggt gaacggctgc
    12001 tccgggacga tgagcgcgca cgcgcgccgt tcccacgcgt agcgcagcgc tgccgagatc
    12061 atccagcccc ctcgcgcgcc gcccggagcg aggagcacga cggtgtcggg gccgaccgag
    12121 ccgatgtcct cgaagtcggc gacgagggcg agatcgcgca cggggctgcg gcccgagacg
    12181 aggtgcagca ccgagaggcc ggcgagatcg ggcgtgtcga tgaggtcgcg cagctcgacg
    12241 gctcccgcgg aggatcggtt catcgcgggg cccttccgcg gtcggggagc agggagtgca
    12301 gcgtctccgg ggccgccaga cgccggccct ccggcgtgta ccactggccg gcggcaggca
    12361 ggacgacgag gtcgagggtg ttgccgaccc acaggtcgtc gaggctggcg acgcggccgt
    12421 cgtcgggatg cagcatcgtg atgatgtccg ggatgaccgc ttcgggcgtg ccgtcgacca
    12481 tgagcagaag aagttcgttc tggatctcca gctgcacgaa gcgcccctgc gactcctcga
    12541 tgagcacgac gctcgacgga tggtccggtt cgcccgccgg agcgggtcgc gagagcccgg
    12601 cgacgtcggc gactctcgcc cggatgatcc ggcgaacgcc ctcgctgcgc cggagctgct
    12661 cgtgcttcgc actcaccctc gcgtcggagt ccaggatcct gccgatgcgc accatccgcg
    12721 acacgctgcc gagtactccg gtccgggcga gatccctcgc cgtcatcgga tagagcgccg
    12781 tcgctgacca gccgccgaat tcctcggcga gcgcccggac gaggcgctcc gcacgccgag
    12841 ggtcgcggac ccgcaggaac gcggactcgc cgactggtcc cgtcgcggcg agggggccgg
    12901 ccgggagccc cgcgagggtg aacacggact ggaacagcag cgggaacacc cgccccatgg
    12961 ggtcggcgtc gaccactggg cggccgagct gcatcccggt gagcaggggg accatcgagt
    13021 tcacgttcgc cgcggcgaga gggaacagtc cctcgatgcg gcgccccagc tcgcgctcga
    13081 gcagctcgac gctgctgacg aactcgtcgc cggcagggcg cagctccgag agcgggagcc
    13141 cgttgttcac gaacccgact gctgccacca gcgcgtcctc gtcgaattcc tcgacgccga
    13201 gcagctccgg ggcgacgccg ccctccatcc gcgcgaggat ccgatcctgc tgctcctcgc
    13261 accattcggg atcggccgcg caggcgagga agagcgcgcc ggtgcgcaga tgcctgacgt
    13321 cctcagcgct gagccgcatg gcgcaccttc ctgccggcga gcagaccccc gacgggatcc
    13381 ccggcgacgc cgcgcacgcg gactgagacc accctggcgt gctcataggt cgtggccacg
    13441 agctgcgact cgaggatgcg cacagaggac gggagtgcgc cgaacgccgc gagcctggca
    13501 cggacgcgcg cctcggccgc ctgcagctcg tgcgccatct cggcggcgtt cacggcgccg
    13561 acggtgccga gcgcccaatc ggagagcgga gcgcaggctg ctcccagcgc gcggagatcg
    13621 tggtccgtct ccacgacctc gccgccatcg agccagcccg gcagcggggc tccggcgacg
    13681 cggacgaggt gggggatcgt ggtcctcccg tgcaggagga tctcggtcgc cgggaccagg
    13741 tgcgcggcct gggtcgacag gcgggctccc ttccagcgca gccgcgggat cactgccggc
    13801 gctccggcgc gcagcgtccc cagcgccagt ccgtcatccg acgccagcac gagatcgccg
    13861 tcgtccaacc cgcagagcgc cgcggcgccg aggaaatcca ccggtgcgcc cgagaacgcg
    13921 gagtggatcg gctctgcggg gagcctgctc agcggcacgc ggcccccgtc attcctcgtg
    13981 accgagaggc gcgcgtcggg gaacctgccg ccggcgacca gtgcgagcgc ggtggcgagt
    14041 gcctcggcgt cgggcacgag ggcgtcgttg aggatcgcgg tgtgctcccg cgtcgcgaag
    14101 gagttgcttg cgaaggcgtg cgagaacacg acgctcagcg gaccgccatg ctcgaggagg
    14161 atctcgcccg cggcgagctc gtgcgccggg ttcacgagcg cgccgacgct cgtgatcacg
    14221 acgcgggacc ccggcgggat cgcggcggcg ga
//
LOCUS       DATMSX010000353         8637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_175864,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000353 DATMSX010000000
VERSION     DATMSX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175864"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..564)
                     /locus_tag="VN041_11005"
     CDS             complement(46..564)
                     /locus_tag="VN041_11005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWU29601.1"
                     /translation="MNPELARAESLIRSIPDYPQPGILFRDITPLLADARALHATTEA
                     LVEPFAGSFDVVAGVEARGFILAGAAAITAGVGFVPIRKAGKLPQPAAAVDYALEYGT
                     ATIEMHDDLPAGTRILLIDDVLATGGTLAAGRKVIESLGYTVAGISVLFEIEGLGGRD
                     VIGDLHTVFRSS"
     gene            640..2061
                     /locus_tag="VN041_11010"
     CDS             640..2061
                     /locus_tag="VN041_11010"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWU29602.1"
                     /translation="MTRSFPDDFLFGAATAAYQIEGAAFEDGRTASIWDAFARVPGAV
                     VNADNGDIACDHYHRYRDDVALMKELGLQTYRFSTSWSRVRPDGGAVNAKGLDFYSRL
                     VDELLGAGIVPWLTLHHWDMPQALEEQGGWTVRDIADRFTEYALSVHDALGDRVQHWT
                     TMNEPWCSSFLSYTAGVHAPGRTSIRDGLLASHHLLLAHGQAVQALRARDADLDLGIT
                     LNLTVADPADADDPLDIAAARRIDGQFNRWFLDPLFLGEYPSDIVRDIRAVDPAAISD
                     WQDAVHDGDLAVISTPLDALGINYYHGELLSGHPQPGGEGDPHETPGQQRETASPFPS
                     HDDIHWVERGLPRTDMGWEVQPEGLTRLLRRVADEYSGAVPLYVTENGVAYDDVVGSD
                     GEVPDAGRVAFLRDHVAATLDAIEQGVDVRGYFYWSLMDNYEWAWGYAKRFGIVRVDY
                     ETQRRTVKESGREYARIIAARAI"
     gene            2132..3133
                     /locus_tag="VN041_11015"
     CDS             2132..3133
                     /locus_tag="VN041_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29603.1"
                     /translation="MMPRSTIEEVAALAGVSRSTVSRVVNGSTAVSPEALSAVQTAIA
                     QLNYAPNRAARSLASRQTYAIGLVVPEDTTRFFGDPFLASVVAGISARLADSDYILNL
                     LIASDDPGGKMVRFVRNGGVDAAIIVSHHTSDSFVGSIADAVPVVFGGRRSNAQDSDI
                     IIDVDNVAAARDAVAHLIALGRRRIATITGTMTMSVSIDRRDGFLAALADAGLEPAGE
                     VDGDFSEQGGADAAARLLEQPERPDAIFIASDLMARGAIGVLRSAGLRVPEDVAVIGF
                     DDSALAAASDLTSVRQPMFHQGEMLAGMIIDVLAGRRPPPLTILPTELVVRGSAPAV"
     gene            complement(3199..4014)
                     /locus_tag="VN041_11020"
     CDS             complement(3199..4014)
                     /locus_tag="VN041_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HWU29604.1"
                     /translation="MRIKRTIALAATAAAALSLGAAIPAAAATPTVDSGIPSYQEFAS
                     STYRDIDGGYVVNGDELVSGQGELHAFYDRLVGSDSLTDGLIVNTVNGVDDKWSAAQV
                     GNLTYCVSTKFGARHDDIVAAMESGAALWESASSAIDFVYVSSADGNCTTRNKSVLFS
                     VEPVQTSQYIARAFFPSTPDRQMNVLVDDSIWSSGSWTPTNILGHELGHTLGFRHEHT
                     RPESGTCFEDNNWRPLTPYDSSSIMHYPQCNGTSDDLSMTSTDRAGVVALYGN"
     gene            complement(4365..5984)
                     /locus_tag="VN041_11025"
     CDS             complement(4365..5984)
                     /locus_tag="VN041_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29605.1"
                     /translation="MRPSPARAPRSSATWKFRRGTTGSALLALTVGSTLALAGASAAT
                     ASPADDAGPRSVTALVPATPEVSATIAAFAPHGCTDAALPITVEESRLTAVLPDAADP
                     ARPAERTFARTMVEGAGLGEWTSSFAGRLCGQKTLAKATETVKRLGTHLWRDAVTRAQ
                     STGPVEGVLPAGDDRPLYWTRIEAMSVLHQWKAGFAMTTEQRAELVEAFDRASRGMGD
                     ISFPAGNVKRVLVSGFDPYTLDGGKTGSAPGTVGNNIRHGNPSGATALSIDGTTTTEA
                     DGTVIFYEAYTLPVSYPEFERGYLEDTVGPFMRPGKRQLDASITVSQAGGSQFNLEQW
                     NGRYHGPTLGNDRYTPCTTIGGVPELAVDNIGCNTQVVDRWGGGDTLTDPPQWTSATL
                     PMEQMIAARTGSDIPRPPGDTWPDESVAFGAVWNTNYSYFADCSSPVVTSVNQREVAY
                     PPSVAPTPPPADSCARSGAGGSYLSNESAYRNTLLRDRMGLTIPAGHIHTPDMQHFES
                     DFGVSDATFDAWRSAIAGQARELIQVVGENS"
     gene            complement(6082..6720)
                     /locus_tag="VN041_11030"
     CDS             complement(6082..6720)
                     /locus_tag="VN041_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29606.1"
                     /translation="MPTHTPLVDILRERIIDGDFAPGSRLSESTLAEQLEVSRNTLRE
                     AFRVLAEQGLIEHVPHRGVSVASPTIADVIDIYRARRVIECTALRHSEPEHPAVRLMK
                     ESLADAEEHLPAEDWRRVGSANMAFHDAIVALADSPRLARTYRDVAAELRLVFLEIDD
                     PRALHEPFVRKNRAVLETLLERGPAAAADELERYLMASERTVLGAFTRMGKA"
     gene            6834..8111
                     /locus_tag="VN041_11035"
     CDS             6834..8111
                     /locus_tag="VN041_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HWU29607.1"
                     /translation="MSEQTPPALSAEDEARLAAAKKRVGRSAVIGAIFLMATSAIGPG
                     FITQTATFTAKMGSAFAFAILLSVIIDICVQLNIWRMITSSGKRAGELANSAIPFSGH
                     VIAVLVIIGGLAFNIGNIAGGGLGLNALLGVDPKIGGLITGALAIIIFLIKKAGPVMD
                     VVLIILGVGMIIMTVIVAIVSGPPIGDALRQTIVPDTINFATITTIVGGTVGGYITYS
                     GAHRYLASGHTGPEHAGPVMRAAANGVFVTGVMRYVLFLAILGVVASGVTLDLSSQAA
                     NPAGQAFGAVLGDAGLRIFGAIFWAAALSSVIGAAYTSATFLSTFNKRFLGGWSLQWA
                     TVAFIVVSWLIYLLIGTAPAAILVFVGGFNGLILPIGLTVFMYIGWFRRDLLGALKYP
                     LWLLIAGTLVTLLTWYMGAVSIGPIFAFLGVGA"
     gene            8146..>8637
                     /locus_tag="VN041_11040"
     CDS             8146..>8637
                     /locus_tag="VN041_11040"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HWU29608.1"
                     /translation="MASIDLNSDLGENTPDRIVGDDAAMLEIVTSANVACGFHAGSPE
                     GIRSTLDAAVRNGVVIGAHPGYRDYENFGRTNVEIDSATLQAHVEYQLGALEGLTKAA
                     GGRVAYVKPHGALYNTIARDERQAADVVAAIKAIDPNLVLLGLAGGVVLEVASRAGLA
                     VAAE"
BASE COUNT         1287 a         2961 c         2999 g         1390 t
ORIGIN      
        1 agccgacatc gcgcggccgc ccgcggcgcg cggctgacgc atccgtcagg acgagcggaa
       61 gaccgtgtgc aggtcgccga tcacgtcgcg cccgccgagt ccttcgatct cgaacagcac
      121 gctgatcccg gcaaccgtgt agccgaggct ctcgatcacc ttgcggccgg cggcgagcgt
      181 gccgccggtg gccagcacgt cgtcgatcag aaggatccgg gtgccggccg ggaggtcgtc
      241 gtgcatctcg atcgtggcgg tgccgtactc gagggcgtag tcgacggccg ccgcgggctg
      301 cggaagcttg ccggccttgc ggatcggcac gaagccgacg ccggcagtga tcgcggcagc
      361 gccggcgagg atgaagccgc gcgcctcgac gccggcgacc acgtcgaagg agccggcgaa
      421 cggctcgacg agtgcctcgg tcgtggcgtg cagcgcccga gcatcggcca gcagcggggt
      481 gatgtcgcgg aacaggattc cgggctgcgg gtagtccggg atcgaacgga tcagggactc
      541 ggcgcgcgcc agttcggggt tcacccgcca aggctaccgg accgacctcg gagtgacatg
      601 cgtgggagcg ctcccagcca gtcgtggcat cataagagga tgacccgatc cttccccgac
      661 gacttcctgt tcggcgctgc gacggccgcc tatcagatcg agggtgcggc cttcgaggac
      721 ggacgcaccg cgtccatctg ggacgcgttc gcccgcgtgc ccggtgcggt cgtgaacgcc
      781 gacaacggcg acatcgcctg cgaccactac caccgctacc gcgacgacgt ggcgctcatg
      841 aaggagctcg gtctgcagac ctaccggttc tccacgtcgt ggtcgcgcgt gcggccggac
      901 ggcggcgcgg tcaacgcgaa gggtctcgac ttctacagcc ggctcgtcga cgagctgctc
      961 ggcgccggca tcgtgccgtg gctgaccctg caccactggg acatgccgca ggcgctcgag
     1021 gagcagggcg gctggaccgt ccgcgacatc gcggatcgct tcaccgagta cgcgctcagc
     1081 gtgcacgacg ccctgggcga ccgcgtgcag cactggacca ccatgaacga gccgtggtgc
     1141 tcgtcgttcc tctcgtacac cgccggcgtg cacgcgcccg gtcgcaccag catccgcgac
     1201 ggcctgctcg cgtcccacca tctgctgctc gcacacggcc aggccgtgca ggcgctgcgc
     1261 gcgcgcgatg ccgacctcga tctcgggatc acgctgaacc tcaccgtcgc cgacccggcc
     1321 gacgccgacg atccgctcga catcgccgcc gcgcgccgca tcgacggaca gttcaaccgc
     1381 tggttcctcg atccgctctt cctcggggag tatccgagcg acatcgtccg cgacatccgc
     1441 gcggtcgacc ccgccgcgat ctccgactgg caggatgccg tgcatgacgg cgacctcgcc
     1501 gtcatctcca cgccgctgga cgcactgggc atcaactact accacggcga gctgctgtcc
     1561 gggcatccgc agcctggcgg cgagggcgac ccgcacgaga cgccgggcca gcagcgggag
     1621 accgcctcgc cgttcccctc gcacgacgac atccactggg tcgaacgcgg tctgccgcgc
     1681 accgacatgg gctgggaggt gcagcccgag ggcctcaccc ggctgctccg ccgcgtcgcc
     1741 gacgagtact ccggcgccgt tccgctgtac gtcaccgaga acggcgtcgc gtacgacgac
     1801 gtggtcggga gcgacgggga ggtgccggat gccggccgtg tcgcgttcct gcgcgatcac
     1861 gtcgcggcga cgctggatgc catcgagcag ggcgtcgacg tgcgcggcta cttctactgg
     1921 tcgctgatgg acaactacga gtgggcctgg ggctacgcca agcgcttcgg catcgtgcgc
     1981 gtcgactacg agacccagcg gcgcacggtc aaggagagcg gacgggagta cgctcgcatc
     2041 atcgccgccc gggcgatctg acccccaaca gggtcttcga agcacctggg tcgctgagcc
     2101 tgtcgaagcg tcgcccaccc gaaacgagac gatgatgccc cgctccacga tcgaagaggt
     2161 cgccgcgctc gccggcgtct cgcgctcgac cgtgtcccga gtcgtgaacg gctccacggc
     2221 ggtcagcccc gaggcgctct ccgccgtgca gacggccatc gcccagctga actacgcgcc
     2281 caaccgtgcc gcccgctccc tcgccagccg gcagacctac gcgatcgggc tggtggtccc
     2341 cgaggacacc acgcgcttct tcggcgaccc gttcctggca tccgtcgtcg ccgggatcag
     2401 tgcgcgcctg gcggactcgg actacatcct caacctgctc atcgccagcg atgacccggg
     2461 cggcaagatg gtgcgcttcg tgcggaacgg cggggtggat gccgcgatca tcgtgtcgca
     2521 ccacaccagc gactcgttcg tcgggagcat cgccgacgcc gtgccggtgg tgttcggcgg
     2581 tcgccggtcg aacgcgcagg acagcgacat catcatcgac gtcgacaacg tggctgctgc
     2641 ccgtgatgcg gtcgcccatc tgatcgcgct cggacgccgt cgcatcgcga cgatcacggg
     2701 caccatgacc atgtcggtca gcatcgaccg gcgcgacggc ttcctggccg cgctggcgga
     2761 tgccggactc gagcccgcag gcgaggtcga cggcgacttc agcgagcagg gcggggcgga
     2821 tgccgccgcg cgtcttctcg agcagcccga gcgtccggat gcgatcttca tcgccagcga
     2881 tctgatggcc cgcggtgcga tcggcgtgct gcgctcggcg gggctgcgcg tcccggaaga
     2941 tgtggcggtg atcggcttcg acgactccgc cctggccgcg gcatccgatc tgaccagcgt
     3001 gcgccagccg atgttccatc agggcgagat gctcgccggc atgatcatag acgtgctcgc
     3061 aggacgacgc ccgccgccgc tgacgatcct gccgaccgag ctggtcgtgc gaggatcggc
     3121 gcccgcggtc tgaggctcgg acgcgaaccg ggcccggtcg cgaacgaccg ggcccgggtg
     3181 gaacgacggg ggagtgcgtc agttcccgta gagagcgacc acgcctgcgc ggtcggtgct
     3241 cgtcatcgac aggtcgtccg aggtgccgtt gcactgcggg tagtgcatga tcgacgagga
     3301 gtcgtagggc gtgagcggac gccagttgtt gtcctcgaag cacgtgcccg actcggggcg
     3361 cgtgtgctcg tggcggaagc cgagcgtgtg cccgagctcg tggccgagga tgttcgtcgg
     3421 cgtccaggag cccgacgacc agatcgagtc gtcgacgagg acgttcatct gacggtccgg
     3481 cgtgctcggg aagaacgcgc gagcgatgta ctgggaggtc tggaccggct cgaccgagaa
     3541 cagcaccgac ttgttgcgcg tggtgcagtt gccgtcggca ctgctgacgt agacgaagtc
     3601 gatcgccgag gatgcggact cccacagggc cgcgccgctc tccatcgcgg cgacgatgtc
     3661 gtcgtgacgc gcgccgaact tggtgctcac gcagtaggtg agattgccga cctgtgcggc
     3721 cgaccacttg tcgtcgacgc cgttgacggt gttgacgatc agtccgtcgg tgagcgagtc
     3781 ggacccgacg aggcggtcgt agaaggcgtg cagctctccc tgacccgaga cgagctcgtc
     3841 gccgttgacg acgtagccgc cgtcgatgtc gcgataggtc gatgaggcga actcctgata
     3901 gctcgggatg ccggaatcga cggtgggcgt cgccgcggcg gcggggattg ccgcgccgag
     3961 gctgagtgcg gcggctgcgg tggcagccag cgcgatcgtt ctcttgattc gcatggaatg
     4021 ctccttgtga actgagcgat tccgcacgcc tggtcggcga gcggacctcg gatccggccg
     4081 gtgagcgtct ttgcgtcgcc gtccgactcc gaagcccgct caacgtaacg agcgtttttg
     4141 gaccctgtca agcaactgga gaatgggccg cggacatctg gcggatgggt tccaggacgg
     4201 cgcgttcacc tgtccgttcg tcatgcagat gctgtccatt tgtcatgcat ccgctgtctg
     4261 ttcgtcatga gggccgcacc gcgaggaacg ccggaagcgg gcgggttgcg aaacccgccg
     4321 cgctcccggc gctcctcgat gctcagcggt ctgcggaacc gcgctcagct gttctcgccg
     4381 accacctgga tcagctcgcg ggcctgcccc gcgatcgcgc tgcgccacgc gtcgaacgtg
     4441 gcatcgctca ctccgaagtc cgactcgaag tgctgcatgt ccggcgtgtg gatgtgtccg
     4501 gcaggaatgg tcagccccat ccggtcgcgc agcagcgtgt tgcggtaggc cgactcgttc
     4561 gacaggtacg aaccgccggc gccgctgcgc gcgcacgagt cggccggcgg cggagtgggg
     4621 gcgaccgacg gcgggtacgc gacctcgcgc tggttcacgc tcgtcacgac gggtgaggag
     4681 cagtcggcga agtacgagta gttcgtgttc cacaccgccc cgaaggcgac ggactcatcc
     4741 ggccaggtgt cccccggagg gcgcgggatg tcggagccgg tgcgggccgc gatcatctgc
     4801 tccatgggca gggtcgcgga ggtccactgc ggcgggtcgg tgagggtgtc cccgccgccc
     4861 catcggtcga cgacctgcgt gttgcagccg atgttgtcca cggcgagctc cggcacgcct
     4921 ccgatcgtcg tgcacggggt gtagcgatcg ttgccgagag tggggccgtg gtagcgcccg
     4981 ttccactgct cgaggttgaa ctgcgagccg ccggcctggc tcaccgtgat cgatgcgtcg
     5041 agctggcgct tgccggggcg catgaacgga ccgacggtgt cctcgaggta gcccctctcg
     5101 aactcggggt agctcacggg cagcgtgtag gcctcgtaga agatgaccgt gccgtcggcc
     5161 tcggtcgtcg tcgtgccgtc gatggacagg gccgtcgctc cggacgggtt gccgtgacgg
     5221 atgttgttgc ccaccgtgcc gggcgccgag ccggtcttcc cgccgtcgag cgtgtacggg
     5281 tcgaagccgc tgaccaggac gcgcttgacg tttccggcgg ggaacgagat gtcgcccatg
     5341 ccgcgcgacg ccctgtcgaa cgcctcgacc agctctgcac gctgctcggt ggtcatggcg
     5401 aagcccgcct tccactggtg cagcaccgac atcgcctcga tgcgcgtcca gtacagcggc
     5461 cggtcgtcgc cggccgggag cacgccttcg accgggccgg tcgactgggc gcgcgtgaca
     5521 gcatcccgcc acaggtgcgt gccgagtcgc ttcacggtct cggtggcctt ggcgagagtc
     5581 ttctggccgc agagcctgcc tgcgaacgac gacgtccact cgccgagacc ggccccctcg
     5641 accatggtgc gggcgaaggt ccgctccgcg ggccgggccg gatccgcagc atcggggagc
     5701 accgctgtga ggcgcgactc ctcgaccgtg atcgggagcg cggcgtccgt gcatccgtga
     5761 ggagcgaagg cggcgatcgt cgcgctgacc tcaggcgtgg cggggaccag cgccgtcacc
     5821 gagcgcgggc cggcgtcgtc ggcgggggac gccgttgctg ccgatgcgcc cgcgagggcg
     5881 agtgtggagc cgaccgtgag cgccaggagg gcgctgccgg tcgtgcctcg tctgaacttc
     5941 catgtcgccg aactgcgcgg ggcgcgagct ggggaggggc gcatgatcat ccttccgcgt
     6001 tgaagaaacg ttgttcctcg acacggtagg gattgttgaa caagaaagca atgattgttc
     6061 agcggatgga tgcgcgagag ttcaggcctt gcccatgcgg gtgaaggcgc cgagcacggt
     6121 gcgctcggat gccatcaggt agcgctccag ctcatccgcc gccgccgcgg ggcccctctc
     6181 gagcagggtc tccagcaccg cgcggttctt gcgcacgaag ggctcgtgga gggctcgggg
     6241 gtcgtcgatc tcgaggaaca ccaggcgcag ctctgcggcg acgtcgcggt aggtgcgggc
     6301 cagccggggg ctgtcggcca gggcgacgat cgcgtcgtgg aaggccatgt tggcgctgcc
     6361 cacgcggcgc cagtcctcgg ccgggaggtg ctcctcggca tccgccagcg attccttcat
     6421 caggcggacc gccggatgct cgggctcgga gtggcgcagt gccgtgcatt cgatcacgcg
     6481 gcgggcgcgg tagatgtcga tcacatcggc gatcgtgggg gatgccaccg agacgccgcg
     6541 atgcggcacg tgctcgatca ggccctgctc cgcgagcacg cggaacgcct cgcgcagcgt
     6601 gttgcgcgac acctccagct gctcggcgag ggtcgactcg gacaggcgcg aacccggggc
     6661 gaagtcgccg tcgatgatcc tctcgcgcag gatgtcgacg agcggggtgt gcgtgggcat
     6721 agcaccaggg taggggaggg tgcgcgtcat gaaagattgt tcaacaatcc ggcctgatat
     6781 gttctacact cggtgtcatc cgacacccac caacgacgat gggacctccc ctcatgtccg
     6841 agcagacacc tcctgccctc tccgccgagg acgaagcacg cctggccgcc gcgaagaagc
     6901 gcgtgggccg cagcgccgtc atcggcgcca tcttcctcat ggcgacctcc gccatcggcc
     6961 ccggattcat cacccagacc gccaccttca ccgcgaagat gggatcggcg ttcgcgttcg
     7021 ccatcctgct ctcggtgatc atcgacatct gcgtgcagct gaacatctgg cgcatgatca
     7081 cctcttccgg caagcgcgcc ggtgaactcg ccaacagcgc gatccccttc tcgggtcacg
     7141 tcatcgccgt gctcgtcatc atcggcggcc tggcgttcaa catcggcaac atcgccggtg
     7201 gcgggctggg cctgaacgcc ctgctcggcg tcgatccgaa gatcggcggc ctgatcaccg
     7261 gagcgctcgc gatcatcatc ttcctgatca agaaggccgg accggtgatg gacgtcgtgc
     7321 tcatcatcct cggcgtcggc atgatcatca tgaccgtgat cgtcgcgatc gtctccggcc
     7381 cgccgatcgg cgacgccctg cgacagacga tcgttccgga caccatcaac ttcgccacca
     7441 tcaccacgat cgtcggcggc accgtcggcg gctacatcac ctactcgggc gcgcaccgct
     7501 acctggcatc cggccacacc ggcccggagc acgccggacc cgtgatgcgc gcggccgcca
     7561 acggcgtctt cgtcaccggc gtcatgcggt acgtgctgtt cctcgcgatc ctcggcgtcg
     7621 tcgcctccgg cgtcaccctc gacctgtcca gccaggccgc caaccccgcc ggccaggcgt
     7681 tcggcgccgt gctcggcgac gcgggcctgc gcatcttcgg cgccatcttc tgggccgcgg
     7741 cgctcagctc ggtgatcggc gccgcctaca cctcggcgac gttcctctcc acgttcaaca
     7801 agcggttcct gggcggctgg tcgctgcagt gggcgacggt cgcgttcatc gtcgtgtcgt
     7861 ggctcatcta cctgctcatc ggcaccgcgc ccgcggcgat cctcgtgttc gtcggtggct
     7921 tcaacggcct gatcctgccg atcggactga ccgtgttcat gtacatcggc tggttccgcc
     7981 gcgacctgct gggagcactc aagtacccgc tgtggctcct gatcgccggc acgctggtca
     8041 cactgctgac ctggtacatg ggcgccgtct cgatcggacc gatcttcgcc ttcctcggcg
     8101 tcggggcctg agccgcgcac tcatcactca acggaagggg ccgacatggc atccatcgat
     8161 ctgaactccg acctgggcga gaacaccccc gaccgcatcg tcggtgacga cgcggccatg
     8221 ctcgagatcg tcaccagcgc gaacgtggcg tgcgggttcc acgccggcag ccccgagggc
     8281 atccggtcga cgctcgacgc ggctgtgcgc aacggcgtgg tgatcggcgc ccacccgggg
     8341 taccgcgact acgagaactt cgggcgcacg aacgtcgaga tcgattcggc cacgctgcag
     8401 gcccacgtcg agtatcagct gggcgcgctg gagggtctca cgaaggcggc cggcggcagg
     8461 gtcgcgtacg tgaagccgca cggcgcgctg tacaacacga tcgcgcgcga tgagcggcag
     8521 gcagccgacg tcgtcgccgc gatcaaggcg atcgatccga acctggtgct gctgggcctg
     8581 gcaggtggcg tggtcctcga ggtcgcgtcc cgcgccgggc tcgccgtcgc ggccgag
//
LOCUS       DATMSX010000354         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_138091,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000354 DATMSX010000000
VERSION     DATMSX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138091"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..665
                     /locus_tag="VN041_11045"
     CDS             225..665
                     /locus_tag="VN041_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29609.1"
                     /translation="MEKADVIPSIVLSAHALARFAAQDAGNEAPSAQWRALSILEQKG
                     GMRIGELAIAARTTQPGMTRLIGTLEQAGLVTRTTDPADTRATQVHATDAGLAHLAEW
                     RAEMRDTLAPRFAGLADEEWIVLQRAAEILAAHAQETTTTGEAE"
     gene            662..1888
                     /locus_tag="VN041_11050"
     CDS             662..1888
                     /locus_tag="VN041_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29610.1"
                     /translation="MSGTPQGSVWRQPAQVWAVAFACVVAFMGIGLVDPILPAIADSL
                     KASPVETELLFTSYLLVTGLAMLITSWVSSRIGAKATLLTGLALIVVFALLCAMSGGV
                     DWVIGFRAGWGLGNALFISTALATIVGAASGGSGAAIVLYEAALGLGIAIGPLLGGLL
                     GEVSWRGPFFGVVALMAIGFIAVLALVRDGGEKREPMPLSAPFRALGRPALAILAVAA
                     LFYNIGFFVLLAFSPFPLGFGAMGIGLTFFGWGVALAVTSVWVAPALMRRISRTTVIL
                     ITLPLLAIDLIVAGLLVANPTALVICIIVGGLMLGIMNTVLTEAVMEATDLPRSVASS
                     AYSAVRFLGGAMAPPVAAALAHAYNDTVPYLFAAVSVLIAALTILLGRRALSHIDDAG
                     EGAGEDAEAILMGDAA"
BASE COUNT          281 a          739 c          655 g          328 t
ORIGIN      
        1 gtcgccgggc gccgcgcccg gctgcggcgc gcacgccgtg aacacgaggg ccgcggtggc
       61 gagcaccgag ccgaggagca acttcttcgt aacccgcatc tggatgcctt tcaacggtgg
      121 aaggtcggga acatgaaacc cgattcaggt attggaatcg taagccctgc ggcatccgcc
      181 gtcaaacgat ccccattcat accaagtgat agatactcag tggtatggaa aaagcggacg
      241 tgatcccgtc gatcgtgctc tccgcgcacg ccctcgcgcg gttcgccgcg caggatgccg
      301 ggaacgaggc gccctcagcg cagtggcgcg cgctcagcat cctcgagcag aagggcggca
      361 tgcgcatcgg cgagctcgcg atcgccgccc gcaccacgca gccaggcatg acccggctga
      421 tcgggactct cgagcaggcc ggcctcgtca cccgcaccac cgaccccgcc gacacgcggg
      481 cgacgcaggt gcatgcaacg gatgccgggc tcgcccacct cgccgagtgg cgggcggaga
      541 tgcgcgacac gctcgcgccc cgcttcgcgg gactcgccga cgaggagtgg atcgtgctgc
      601 agcgcgcagc agagatcctc gccgcacacg cacaggaaac caccacgaca ggagaagccg
      661 aatgagcgga acaccacagg gatcggtctg gcgccagccc gcgcaggtat gggcggtcgc
      721 cttcgcctgc gtggtcgcct tcatgggcat cggcctggtc gacccgatcc tccccgccat
      781 cgccgactct ctgaaggcct cccccgtcga gaccgagctg ctgttcacca gctatctgct
      841 cgtgacgggg ctcgcgatgc tcatcaccag ctgggtctcc agccgcatcg gcgccaaggc
      901 gacgctgctg accggcctcg ccctcatcgt cgtgttcgca ctgctctgcg ccatgagcgg
      961 cggcgtcgac tgggtgatcg gcttccgcgc cggctggggt ctgggcaacg ccctgttcat
     1021 ctccacggcc ctggccacca tcgtcggcgc ggcctcgggt ggcagcggtg cggcgatcgt
     1081 gctctacgag gcggcactcg gcctcggcat cgctatcggc cctctgctcg ggggcctgct
     1141 gggcgaggtc agctggcgcg gaccgttctt cggcgtcgtc gcactgatgg ccatcggatt
     1201 catcgccgtc ctggcgctcg tgcgcgacgg cggcgagaag cgcgagccca tgccgctgtc
     1261 cgccccgttc cgcgctctcg gacgcccggc actggcgatc ctcgccgtcg cggcgctgtt
     1321 ctacaacatc ggcttcttcg tgctgctggc cttctcgccg ttccccctcg gcttcggcgc
     1381 catgggcatc ggcctcacgt tcttcggctg gggtgtcgcc ctcgccgtca cgagcgtgtg
     1441 ggtggccccc gccctgatgc ggcgcatctc gcgcacgacc gtcatcctca tcacgcttcc
     1501 cctgctcgcg atcgatctga tcgtggccgg actgctcgtg gcgaacccca ccgcgctcgt
     1561 gatctgcatc atcgtcggcg gcctgatgct cggcatcatg aacaccgtcc tgaccgaggc
     1621 cgtcatggag gcgaccgacc tgccgcgttc cgtggcgtcg tccgcatact ccgcggtgcg
     1681 gttcctcggc ggggcgatgg cgccgccggt cgccgcagct ctcgcgcacg cctacaacga
     1741 cacggtgccc tacctgttcg ccgccgtgtc cgtgctgatc gccgcgctca ccatcctgct
     1801 cgggcgccgc gcgctctccc acatcgacga cgccggtgag ggcgcgggcg aggacgccga
     1861 ggctatcctc atgggtgacg ccgcctgagc ggcatccgac cgaaggagcc gcccgtgaac
     1921 gcacccgacg atctgcccgc gcgcagccgg gtggtgcacg atcacctcgc ggccgccggg
     1981 gtcgacagtg cgatcgtcgt gct
//
LOCUS       DATMSX010000355         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_62730,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000355 DATMSX010000000
VERSION     DATMSX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62730"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN041_11055"
     CDS             <1..378
                     /locus_tag="VN041_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWU29611.1"
                     /translation="GIMRALGLAASRAVQGVVDQGAALERALVILDGNHDYVSRVHPA
                     RLSVRTIVKGDRDCASVSAASVIAKVARDALMTDLHPEHPAYQWDRNKGYASPEHRAA
                     IRDQGLSPLHRSSWAIADAPTLF"
     gene            408..734
                     /locus_tag="VN041_11060"
     CDS             408..734
                     /locus_tag="VN041_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HWU29612.1"
                     /translation="MDEDAFDDYDRELELALFREYRDVVAQFQYVVETERRFYLANEV
                     NVVRRDTENDFYFEISMTDVWVWDIYRADRFVKAVRVLTFKDVNVEELQRREFELPDE
                     LSLDGK"
     gene            832..1194
                     /locus_tag="VN041_11065"
     CDS             832..1194
                     /locus_tag="VN041_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWU29613.1"
                     /translation="MAAKDELGRAGEERATQHLRSRGYAILDRNWRCEQGEIDIVAFR
                     DDRLCIVEVKTRRSELYGHPFDAIDERKRRRLWRLAFAWAAAHPDAARRREIRLEAIG
                     LVGADASTARLEHLVDIL"
     gene            1191..>1596
                     /locus_tag="VN041_11070"
     CDS             1191..>1596
                     /locus_tag="VN041_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HWU29614.1"
                     /translation="MSTARTWAVALTGVDGHMVEVEADFTAQTPEFRIIGLPDKALGE
                     AVQRVRNASSNAGLDVPRRRLTVNLSPASLPKHGSGFDLSIAVATAAAGGALPAEAIR
                     STVHIGELGLDGRLRPVPGVLPSVYAAARAGFG"
BASE COUNT          248 a          548 c          552 g          248 t
ORIGIN      
        1 gggatcatgc gcgccctggg gctggccgcc tcgcgcgcgg tgcagggcgt cgtcgaccag
       61 ggcgctgcgc tggagcgcgc cctggtgatc ctggacggga accacgacta cgtctcccgg
      121 gtgcacccgg cgcggctgtc cgtgcgcacc atcgtgaagg gcgatcgcga ctgcgcatcg
      181 gtctctgcgg catccgtgat cgcgaaggtc gcccgcgacg cgctgatgac ggatctgcat
      241 cccgagcatc ccgcctacca gtgggaccgc aacaagggct acgcgagccc cgagcaccgc
      301 gccgcgatcc gggaccaggg cctttcgccg ctgcacaggt cgtcgtgggc gatcgccgac
      361 gccccgacgc tcttctagcg cgcactctct cctaggatgg aagcatcatg gatgaggatg
      421 ccttcgacga ctacgaccgc gagctcgagc tcgcgctgtt ccgcgagtac cgtgacgtcg
      481 tcgcgcagtt ccagtacgtc gtggagaccg agcggcgctt ctacctcgcc aacgaggtga
      541 acgtcgtacg tcgcgacacc gagaacgact tctacttcga gatctcgatg accgacgtgt
      601 gggtgtggga catctaccgc gccgaccgct tcgtgaaggc cgtgcgagtg ctgacgttca
      661 aggacgtcaa cgtcgaggaa ctgcagcgcc gcgagttcga gctgccggac gagctctcgc
      721 tcgacggcaa gtgagcccac tggtttccac attccgctga cgcggacggg ctcttcacag
      781 atgccgtgat ccgctgcgcc cggcgtcggc gcgccccgtc aggctgtcgt catggcagcg
      841 aaagacgaac tcgggcgcgc cggcgaagag cgggcgacgc agcatctccg ctcacgggga
      901 tacgccatcc tcgaccgcaa ctggcggtgc gaacagggcg agatcgacat cgtcgcgttc
      961 cgtgacgatc ggctgtgcat cgtcgaggtg aagactcgcc gatccgagct ctacgggcat
     1021 ccgttcgacg cgatcgacga gcgcaagcgg cgacggctgt ggcggctggc gttcgcctgg
     1081 gccgcagcgc atccggatgc cgcacgacgt cgcgagatcc gcctggaggc catcggcctg
     1141 gtgggagcgg atgcctcgac cgcccggctc gagcacctcg tggacatcct gtgagcacgg
     1201 cgcgcacctg ggccgtcgcc ctgacgggcg tggacgggca catggtcgag gtcgaggccg
     1261 acttcaccgc gcagacgccc gagttccgga tcatcgggct gccggacaag gcgctcggtg
     1321 aggcggtcca gcgggtgcgc aatgcctcga gcaacgcagg actcgacgtg ccgagacgcc
     1381 ggctcaccgt gaatctgtca cccgccagcc tgccgaagca cggctcgggc ttcgatctca
     1441 gcatcgctgt cgccaccgcg gcagccggcg gcgcgcttcc cgccgaggcg atccgctcca
     1501 ccgtgcacat cggcgagctg ggtctggacg gcaggctgcg cccggttccc ggagtgcttc
     1561 catcggtgta cgcggcggcg cgcgcgggct tcggcc
//
LOCUS       DATMSX010000356         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_100194,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000356 DATMSX010000000
VERSION     DATMSX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100194"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..938)
                     /locus_tag="VN041_11075"
     CDS             complement(3..938)
                     /locus_tag="VN041_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWU29615.1"
                     /translation="MAEVPSTGAAEGAFTWVEFVDRDQRDVLAEVRRLSAHPAMFEDS
                     LGRGRPPRLEVLDGQLLVIAWDLDPDKATSKPGDVVILAGDSRLLTAQWDGDGPARLL
                     RPLIEREHPSGATPLGAAYRILAAVVQSYEATVASIENELDEVETEVFDPAVDEDYQR
                     IYRLRRRIGHVDRAASALRQGFETNADALDDLLEDEPTLHAYFRHMRSDLAALADLLS
                     TQRMALDAVVSSHESNVSSRQNRDMRTIAAVAALLALPTVVAGIYGMNFKDMPLLHIP
                     VGWVFVLIGTVALDVAAVLVFRRRGWLGADHGRDR"
     gene            complement(944..1108)
                     /locus_tag="VN041_11080"
     CDS             complement(944..1108)
                     /locus_tag="VN041_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29616.1"
                     /translation="MNLLFIIIAVVAIILAITGGLVQSLNFLLWVGLVLLALAVIAWL
                     IRAISGRNAA"
     gene            complement(1224..1535)
                     /locus_tag="VN041_11085"
     CDS             complement(1224..1535)
                     /locus_tag="VN041_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29617.1"
                     /translation="MGLLYYGGTSDPIEIEDRALAHLKVVIATKLRRNESFTLSWKHP
                     EGGAPGRSTIWLHPSIPLRFVFEAPEPPELHAEWITAMAHSANTSGGITLVPEDMVPS
                     E"
     gene            1600..1953
                     /locus_tag="VN041_11090"
     CDS             1600..1953
                     /locus_tag="VN041_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU29618.1"
                     /translation="MGKFVYENGVKTEIEDRALMHLQLVITAKLRRGEPFPFSWREDV
                     SVGGGRTTVWMHPGSSLVFKYYGSRQPSINRGWTEALAFTANSPGGLYLVPEPPDAGD
                     GGGSTEELQAPQPEQ"
     gene            complement(1990..2214)
                     /locus_tag="VN041_11095"
     CDS             complement(1990..2214)
                     /locus_tag="VN041_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29619.1"
                     /translation="MSGIDQGMMPEHVQPQHAEDDTGPGEPEQDENGADTGAEDEAEP
                     NREHRAEEPKPVGKDPHPEGEVPHSSQRKP"
     gene            2399..3283
                     /locus_tag="VN041_11100"
     CDS             2399..3283
                     /locus_tag="VN041_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU29620.1"
                     /translation="MSDSTKDPRDAHRDEPMPPQQQEQPGKTDRTDPEPDHGESSYVG
                     HGRLNGRRALITGGDSGIGRAVAIAFAREGADVAIVHLPEEQDDADSTVDLIRQEDRT
                     GLSIPGDLRDEEFATDAVRRARDELGGLDIVVLNAAYQHDIDSFDTLRTEDMRRVFDT
                     NLGGTIFTARAAYPDLEPGAAIIVTASIQAYNPSPGLIDYAMTKAAQVAFVKALAEEA
                     GERGIRVNAVAPGPIWTPLIPATGWDADKLASFGKDTPLGRPAQPAELASAYVHLASD
                     ESSYTSGAVLAVTGGKPL"
     gene            3280..3591
                     /locus_tag="VN041_11105"
     CDS             3280..3591
                     /locus_tag="VN041_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29621.1"
                     /translation="MSTVLSDPPSAPAPRGDWEVTWLGRDSWRICDRARRQGDADCLI
                     AYVERNSTGTLDVLWLRRPCPRRTRFRDFAQLLGDLDAAVSAASDRSRAPSPIPHLPP
                     R"
     gene            complement(3613..3933)
                     /locus_tag="VN041_11110"
     CDS             complement(3613..3933)
                     /locus_tag="VN041_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29622.1"
                     /translation="MGSLYYGDADEPILIEDRALAHLKVVIATKLRRNESFTLSWRHA
                     EGAPVGRSTIWLHPSIPLRFVFDEPEPPPLSRTWIEELAHSANSSGGIALVDEHIENP
                     PPEK"
BASE COUNT          684 a         1484 c         1484 g          651 t
ORIGIN      
        1 gctcagcggt cgcggccgtg gtccgcgccc agccagccgc ggcgccggaa cacgagcact
       61 gccgcgacgt cgagcgcaac cgtgccgatc agcacgaaca cccagcccac cgggatgtgg
      121 agcagaggca tgtccttgaa gttcattccg tagatgcctg cgaccaccgt cggcagcgcg
      181 agcagcgccg cgacggcggc gatggtgcgc atgtcgcggt tctggcgcga cgacacattg
      241 ctctcatggc tggagaccac ggcgtccagc gccatgcgct gcgtcgagag caggtcggcg
      301 agagccgcga ggtcgctgcg catgtgccgg aagtacgcat gcagcgtcgg ctcgtcctcc
      361 agcagatcgt cgagcgcatc ggcgttcgtc tcgaaaccct ggcgcagagc ggatgccgcc
      421 cggtccacat ggccgatgcg ccggcgcagg cggtagatgc gctggtagtc ctcgtcgacc
      481 gcggggtcga acacctcggt ctcgacctcg tcgagttcgt tctcgatcga cgccaccgtg
      541 gcctcgtacg actgcacgac cgctgcaagg atgcggtacg cggcgcccag cggagtggcc
      601 ccgctcggat gctcgcgctc gatgagagga cgcagtagtc gcgccggtcc atcgccgtcc
      661 cactgcgcgg tcagcagccg cgaatcgccg gcgaggatga ccacgtctcc cggcttgctg
      721 gtggccttgt cgggatcgag atcccaggcg atcacgagca gctgcccgtc cagcacttcg
      781 agtctcggag ggcgtccccg cccgagcgag tcctcgaaca tcgccgggtg cgccgacagg
      841 cgcctcacct cggcgagcac gtcgcgctga tcgcgatcga cgaactcgac ccaggtgaag
      901 gcgccttcgg cagcccccgt cgagggcact tccgccatgt gactcaggcc gcgttgcgac
      961 cggagatcgc cctgatcagc caggcgatca ccgccagcgc gagcagcacg agtccgaccc
     1021 agagcaggaa gttcagcgac tgcacgaggc caccggtgat ggcgaggatg atcgcgacca
     1081 ccgcgatgat gatgaacagc aggttcatga cgatcccttc gtccggcggg gcggaggtgc
     1141 cccggctgat cacacgctac gaagaggccg gattctcgaa cagggggttg acgacgggcc
     1201 cgcgaacctg ccattgcggc ggtctactcg gacggcacca tgtcctcggg gaccagggtg
     1261 atcccgccgc tggtgttcgc ggagtgcgcc atcgcggtga tccattccgc gtgcagctcg
     1321 ggaggctcgg gggcctcgaa gacgaagcgc agcgggatgg agggatgcag ccagatggtg
     1381 gagcggcccg gtgcaccccc ttccgggtgc ttccacgaga gcgtgaagct ctcgttgcgc
     1441 cggagcttgg tcgcgatcac gaccttgaga tgtgcgagcg cccggtcctc gatctcgatg
     1501 ggatcggacg ttccgccgta gtagagcagt cccatatcgc ctaacgtaga cctcatacct
     1561 ggaatgcgtc actccgcttc cagcgcacgg aggcagcaca tgggcaagtt cgtatacgag
     1621 aacggcgtca agacggagat cgaggatcga gctctgatgc acctgcagct cgtcatcacc
     1681 gcgaagctcc gacgcggcga gccgttcccg ttcagctggc gtgaggatgt gagcgtcggc
     1741 ggcggccgca cgacggtgtg gatgcatccc ggcagctcgc tcgtgttcaa gtactacggc
     1801 agcaggcagc cctccatcaa ccggggctgg acggaggcgc tggccttcac cgccaactcg
     1861 cccggagggc tgtacctcgt gcccgagccg ccggacgccg gggatggcgg cgggtccacc
     1921 gaggagctgc aggcaccgca gccggagcag tgatcgactg aggcgaccct gccggcaccg
     1981 gctcgacgcc tacggcttcc gctggctgct gtgggggacc tcgccctcgg ggtgaggatc
     2041 ctttccgacc ggcttcggct cctcggcacg gtgctcgcga ttcggctcgg cttcgtcctc
     2101 ggcgccggtg tccgccccgt tctcatcctg ctccggctcg ccggggccgg tgtcatcctc
     2161 cgcgtgctgc ggctgcacgt gctcgggcat catgccctga tcgatgccgc tcatcacgtc
     2221 tcctctccgc cttgggactg cgttcgagtg ccactgtgca acgggtctcg tggcaccgga
     2281 tgcccgttga catcgctttc gcgcgcgcgt acgggaaccg gcgcgggtgt caaccccctg
     2341 gctcccggcg gcagcccatg acagcgtctt cggcatacag accggagagg gatcgacgat
     2401 gagcgacagc accaaggatc cgcgcgacgc gcacagagac gagccgatgc ctccgcagca
     2461 gcaggagcag ccggggaaga ccgaccgaac cgatccggag ccggaccacg gcgagtccag
     2521 ctatgtcggt cacggccggc tgaacggtcg cagggccctg atcacaggtg gcgactccgg
     2581 gatcggccgg gcggtcgcca tcgccttcgc ccgggagggc gccgatgtcg cgatcgtgca
     2641 cctgccggag gagcaggacg acgccgacag caccgtggac ctgatccgcc aggaggaccg
     2701 caccgggctc agcatcccgg gcgacctccg tgacgaggag ttcgcgacgg atgccgtgcg
     2761 acgggctcgc gacgaactgg gaggcctcga catcgtggtc ctcaacgccg cgtaccagca
     2821 cgacatcgac agcttcgaca cgctgcgcac cgaggacatg cgccgggtgt tcgacaccaa
     2881 cctgggcggc acgatcttca cggcccgggc cgcgtacccg gacctcgagc cgggggccgc
     2941 catcatcgtc acggcttcca tccaggcgta caacccctcc ccgggactga tcgactacgc
     3001 catgaccaag gcggcgcagg tcgccttcgt caaggccctc gccgaagagg ccggcgagcg
     3061 cggcatccgc gtgaacgccg tggcaccggg accgatctgg acgcccctga tcccggcgac
     3121 gggatgggat gccgacaagc tcgcgagctt cggcaaggac acgcccctcg gccgccccgc
     3181 gcagccggcg gaactcgcca gtgcgtatgt gcatctggca tccgacgagt cgtcctacac
     3241 ctcaggcgcc gtgctcgccg tgaccggcgg gaagccgcta tgagcaccgt gctctccgac
     3301 ccgccgagcg ccccggctcc gcgcggggac tgggaggtca cctggctcgg gcgcgacagc
     3361 tggcgcatct gcgatcgtgc gcggcgtcag ggcgacgccg actgcctgat cgcctatgtg
     3421 gagcgcaaca gcacgggcac gctcgacgtg ctgtggctgc gcaggccgtg cccgcgccgg
     3481 acccggttcc gtgacttcgc ccagctgctc ggtgacctcg atgcggccgt cagcgccgca
     3541 tccgaccgtt cccgggcccc ctcgccgatc ccgcacctcc ccccgcgttg atcggcccac
     3601 ccgccgccgg agtcacttct ccggcggcgg gttctcgatg tgctcgtcga cgagggcgat
     3661 gccgccgctc gaattcgccg agtgggcgag ctcctcgatc caggtgcggc tcagcggcgg
     3721 cggttccggc tcgtcgaaca cgaaccggag cggaatggac ggatgcagcc agatcgtgct
     3781 gcgaccgacc ggagcgccct ccgcgtggcg ccacgacagc gtgaagctct cgttgcggcg
     3841 cagcttggtg gcgatcacca cttttagatg tgcgagcgcg cggtcctcga tgaggatcgg
     3901 ctcatccgcg tcgccgtagt acaggcttcc cattccgggt cagcgccgtc cgccgcgtga
     3961 gcggccctgg gcggggtgag acgggatcgg atgacgacgg ttcacgtgca ctcctggggg
     4021 atagagcgga tgcccgtcac gaatcggcgg gctacaggaa tctatcgtgg cgcagcttcg
     4081 ccacgatgca gggacgcgac gtgtggacgt tacggcgaaa ggcatgttcg cgccccgtca
     4141 tcaacggatg cggaagttct gtcagcgtcg tcgcccggcg gtcgccgcgg gaggcacgcc
     4201 gtaggcatcg cggtaggccg cggagaaccg ggatgggtgc gcgaaccccc acctgcgcgc
     4261 gacggtggcg atcgagtcgg acgtgcctgc gagcagctcg cgg
//
LOCUS       DATMSX010000357         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_175913,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000357 DATMSX010000000
VERSION     DATMSX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175913"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="VN041_11115"
     CDS             <1..1975
                     /locus_tag="VN041_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU29623.1"
                     /translation="RSERAGRDEARSIRNRAESGRREAPHHRNDRDDRGGRGFDRGER
                     RFDERPRRDDRAGGFRRDERPSRDDRGGRGFERRDDRGGARGGFDRTERPRFDRDDRG
                     GRGFERRDDRPRFDRNDRNDRGGRGFDRDERPARDDRGARGGFERRDERPRFDRTDRP
                     QRTDRFDRNERRDERPRFNRDDRNERPRFERSDRRDERPRFERSDRDARPIRQNRDDR
                     PSRSDWNRTATVKAHEEHVDVVHERLEAEAVQATEVADVTFGDLGMGSNIVEVLKNMG
                     AETPFPIQAATIPAILEGRDVLGRGRTGSGKTIAFGAPLVESILLSQKGKRREIGRKP
                     RAIILAPTRELALQIDRTVQPIARSVGLFTTQIYGGVPQARQVGALNKGVDIIIGTPG
                     RIEDLIEQRKLDLSEIRIAVLDEADHMAELGFAEPVQRILRKTGTALEDGATSQKLLF
                     SATLDREVAAIVDEFLVNPAVYEVAGEDQNSSTIEHRVLVIEHRDKAEVLTSLVDREG
                     QTLVFARTRAYAEMLSEQFEDAGIASVALHGDLNQAKRTRNLQKLTAGKVQVLVATDV
                     AARGIHVDDIDLVVQADAPDEYKTYLHRSGRTGRAGRSGRVVTLITRQRRRRMEDLLN
                     RAEIDAPFEQAAPGDDLIDEITGRVPSAAELTA"
     gene            2096..2758
                     /locus_tag="VN041_11120"
     CDS             2096..2758
                     /locus_tag="VN041_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29624.1"
                     /translation="MPLIRPASLPLIRAAEGGSGDIGQADAAARVLRADYERWSRLGW
                     GTLAYLGAVLGTLVGLAMLEAASELSWGAGRAAVVVIGCVALIIAVTSLLVLYRLWRT
                     GRRLTIAAAWWLRLPFRTGQRSRRAPFWYAPRTVQYEPRILTRTTAGALLFLMAIFGL
                     SSVFFTDAARMPLLTAAMVSIGVISALCMCGILGGIMRITSGLAEGDPLWTGVRDRVR
                     GA"
     gene            2782..2967
                     /locus_tag="VN041_11125"
     CDS             2782..2967
                     /locus_tag="VN041_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29625.1"
                     /translation="MEWGSWATAGGTVWFCIALMTSGLAGVQGRTKLGWFVLGMIFGP
                     LALLLLVFLTEPMVKRD"
     gene            complement(2974..4029)
                     /locus_tag="VN041_11130"
     CDS             complement(2974..4029)
                     /locus_tag="VN041_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HWU29626.1"
                     /translation="MRWQGQTIDDADPSALPGLENRNGIVQSVTTPEFSGMTFHEVLA
                     KSALNAVPGPSRMPFNWTINPYRGCSHACVYCFARGTHQYLDLDAGHDFDSQIIVKTN
                     IVEVLRRELRRGSWQHETVALGTNTDPYQRAEGRYRLMPGIIQALAESGTPMSILTKG
                     TLVRRDIPLLMEAAKRVPIDIQMSIAMYDDALQHSIEPGTPTTQARLDTVRALTDAGF
                     RVGVFLMPVLPHITDSVGAIDEALSRIADAGADHAVYGALHLRAGVKPWFFEWLQRER
                     PDLVASYRGLYPGAAAEAPKGYRQWLAKRMRPLIRTHGLHAQSEEDHDRMRGIMRERM
                     RPTPASAPPAAKAEMLF"
BASE COUNT          642 a         1494 c         1509 g          669 t
ORIGIN      
        1 ccgatctgag cgcgcggggc gcgacgaggc ccgctccatc cggaaccgtg ctgagtccgg
       61 tcgccgcgag gcgccgcacc accgcaacga ccgcgatgac cgtggtggac gtggcttcga
      121 tcggggcgag cgccgcttcg acgagcgtcc ccgtcgcgat gaccgtgccg gtggcttccg
      181 ccgcgacgag cgtccctcgc gcgatgaccg cggtggacgt ggcttcgagc gtcgcgacga
      241 ccgtggtggc gcgcgtggcg ggttcgaccg cactgagcgt ccgcggttcg atcgcgacga
      301 ccgtggcggc cgtggtttcg agcgtcgcga cgaccgtccg cgtttcgacc gcaacgaccg
      361 caacgatcgt ggaggccgcg ggttcgaccg tgacgagcgt cctgcccgcg atgatcgcgg
      421 cgcgcgaggt ggcttcgagc gtcgcgacga gcgcccgcgg ttcgaccgca cggaccgccc
      481 gcagcgcacc gaccgcttcg accgcaacga gcgtcgcgac gagcgtccgc ggttcaaccg
      541 cgatgaccgt aacgagcggc cgcgtttcga gcgcagcgac cgtcgtgacg agcgtccgcg
      601 tttcgagcgc agcgaccgcg atgcccggcc gatccgtcag aaccgcgacg accgtccgag
      661 ccgcagcgac tggaaccgca ccgcgacggt caaggcgcac gaggagcacg tcgacgtcgt
      721 gcacgagcgg ctcgaggccg aggccgtgca ggccaccgag gtcgcggatg tgaccttcgg
      781 cgacctgggc atgggctcga acatcgtcga ggtcctgaag aacatgggcg ccgagacgcc
      841 gttcccgatc caggcagcca cgatccccgc catcctcgag gggcgcgacg tgctcggccg
      901 cggccgcacc ggctcgggca agacgatcgc cttcggtgcg ccgctggtgg agagcatcct
      961 gctgtcgcag aagggcaagc gccgtgagat cggccgcaag ccgcgcgcca tcatcctcgc
     1021 cccgacgcgt gagctggccc tgcagatcga ccgcaccgtg cagccgatcg cccgcagcgt
     1081 gggcctgttc accacgcaga tctacggtgg tgtgccgcag gcgcgtcagg tgggcgcgct
     1141 gaacaagggc gtcgacatca tcatcggcac ccccggccgc atcgaggacc tcatcgaaca
     1201 gcgcaagctc gacctctccg agatccgcat cgcggtgctc gacgaggccg accacatggc
     1261 cgagctgggt ttcgctgagc ccgtgcagcg catcctgcgc aagaccggca ccgcgctcga
     1321 ggacggcgcg accagccaga agctgctgtt ctcggcgacg ctcgaccgcg aggtcgccgc
     1381 gatcgtcgac gagttcctgg tgaaccccgc ggtgtacgag gtcgccggcg aagaccagaa
     1441 ctcgagcacg atcgagcacc gcgtgctggt gatcgagcac cgcgacaagg ccgaggtgct
     1501 cacctcgctc gtcgaccgcg agggccagac gctggtcttc gcccgcaccc gcgcctacgc
     1561 cgagatgctc agcgagcagt tcgaggatgc cggcatcgcg tccgtcgccc tgcacggtga
     1621 cctgaaccag gccaagcgca cgcgcaacct ccagaagctc accgcgggca aggtgcaggt
     1681 tctcgtcgcc accgatgtcg ccgcgcgtgg catccacgtc gacgacatcg acctggtcgt
     1741 tcaggccgat gcgcccgacg agtacaagac gtacctgcac cgttccggtc gcaccggccg
     1801 tgccggccgc tccggccgcg tcgtcacgct gatcacccgt cagcgccgtc gccgtatgga
     1861 ggacctgctg aaccgcgccg agatcgacgc gccgttcgag caggccgccc ccggtgacga
     1921 cctgatcgac gagatcaccg gtcgcgtgcc gagcgccgcc gagctgaccg cctgacgcgc
     1981 gtcacaactc cggagaaacg cccctgtccg gcctgttcca ggccgaacag gggcgtttcc
     2041 gcacccgaat ctccggagtt gcggccgcgt ccggagccgc ccgtagtctg gacggatgcc
     2101 gctgatccgc cccgcatccc tcccgctgat ccgggcggcc gagggcggct cgggtgatat
     2161 cgggcaggcg gatgctgcag cccgcgtact tcgcgcggac tacgagcggt ggagccggct
     2221 gggatggggc acgctcgcct atctcggagc ggtgctcgga acgctggtcg ggctcgcgat
     2281 gctcgaagcg gcgagcgagc tctcctgggg agccgggcgg gctgcggtcg tcgtgatcgg
     2341 atgcgtggct ctgatcatcg ccgtcacaag ccttctcgtg ctgtaccggc tctggcggac
     2401 ggggcgtcgg ctcacgatcg cggcggcctg gtggctgcgg ctgcccttcc gcacggggca
     2461 gcgctcccga cgcgctccct tctggtacgc accgcggacg gtgcagtacg agccccgcat
     2521 cctcacgagg acgacggcgg gtgcgctgct cttcctcatg gcgatcttcg gcctgtcctc
     2581 ggtgttcttc accgacgctg cgcggatgcc gctgctgacg gcggcgatgg tgtcgatcgg
     2641 cgtgatctcc gcgctgtgca tgtgcggaat tctcggcggc atcatgcgga tcacctcggg
     2701 tcttgcggag ggcgacccgt tgtggaccgg cgtgcgcgac cgcgtccgag gcgcctgagc
     2761 ggcagcccat aggctgggcg catggaatgg ggctcttggg cgaccgcggg cggcacggtc
     2821 tggttctgca tcgcgctgat gacgagcggg ctcgcgggcg tacagggccg cacgaaactc
     2881 ggctggttcg tgctcgggat gatcttcgga ccgctcgcac tgctgctcct ggtcttcttg
     2941 accgagccga tggtcaagcg ggactgaccg cgttcagaac agcatctccg ccttcgccgc
     3001 cggcggggcg gatgccgggg tcggccgcat ccgttcgcgc atgatccccc gcatccgatc
     3061 gtggtcctcc tccgactgcg cgtgcagccc gtgggtgcgg atcagcgggc gcatccgctt
     3121 ggccagccac tggcggtagc ctttcggggc ctccgcggcc gcgcccggat agagtccgcg
     3181 gtaggaggcc accaggtcgg ggcgctcgcg ctgcagccat tcgaagaacc agggcttgac
     3241 gccggcgcgc agatgcagcg caccgtacac agcgtggtcg gcaccggcat ccgcgatccg
     3301 cgacagcgcc tcgtcgatcg ctcccaccga atcggtgatg tgcggcagga cgggcatcag
     3361 gaagaccccg acccggaatc cggcgtccgt cagggcgcgc acggtgtcga gccgggcctg
     3421 cgtggtcggc gtgcccggct cgatcgagtg ctgcagcgcg tcgtcgtaca tcgcgatgga
     3481 catctggatg tcgatcggca cgcgcttcgc ggcctccatc agcaggggga tgtcgcggcg
     3541 gaccagcgtg cccttggtga ggatcgacat cggcgtgccg gattccgcca gcgcctggat
     3601 gatccccggc atcaggcggt atctcccctc cgcccgctgg tacggatcgg tgttggttcc
     3661 cagcgcgacg gtctcatgct gccaggaccc gcggcgaagc tcgcgccgga gcacctcgac
     3721 gatgttcgtc ttcacgatga tctgcgaatc gaagtcgtgc cccgcgtcca ggtcgagata
     3781 ctggtgcgtc ccgcgagcga agcagtagac gcatgcatgg ctgcatccgc ggtagggatt
     3841 gatggtccag ttgaacggca tccgcgacgg gccgggaacc gcgttcagcg ccgacttggc
     3901 cagcacctcg tggaacgtca tgccggagaa ctccggcgtg gtcaccgact gcacgatgcc
     3961 gttgcggttc tccaggccgg gcagcgcgga gggatccgca tcgtcgatcg tctgaccctg
     4021 ccaccgcatc tcctcattag aacaaagaaa cgatggccac gcaagcgatg ttcgaacaga
     4081 tctatgatca acgccgggtg cgggccatgg caggatcctg tgcgccggct cccacggact
     4141 tctagggggt ggcggcgagg tcgtgtaacg gtcagggcca tgcgtcacaa tggagcgatg
     4201 agcgccacgc attcccggca tgcgtcgcgt gcgccgctcg ccgtgcgggt cgccgtgccc
     4261 atcggcgtgc tgttcaccgc gatcgcctcg ctggcgacgc tggccggcgg tggt
//
LOCUS       DATMSX010000358         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_33891,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000358 DATMSX010000000
VERSION     DATMSX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33891"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN041_11135"
     CDS             <1..378
                     /locus_tag="VN041_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29627.1"
                     /translation="ANPGPTNPGPTTPTNPVPSDPTPPAPAPPAAPKPLAFIGITPNY
                     VLNILNLPLLTSYTLSLSGQPGAKAAVTYGGLPAGTVTFDSGGRASLVVGASLLGLSN
                     PMIGASYSDGTAGAAIQARRDSI"
     gene            496..1704
                     /locus_tag="VN041_11140"
     CDS             496..1704
                     /locus_tag="VN041_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU29628.1"
                     /translation="MTDFPVPGAASSAPPPPPAPAAPIASAPTAAPGSADAHWDTDAV
                     DATRPSAPPRPPLPTTSTDSGVPVASADRRGNATPEELARAQRILKVISDSYSTKMVG
                     QDRLRSSLLISLIAGGHILLESVPGLAKTTAASTLADTVKASFKRIQCTPDLLPSDIT
                     GSQIYDAATGTFRTVLGPVHANFVLLDEINRSSAKTQSAMLEAMQERQTTIGGEIHAL
                     PKPFLVIATQNPIEQEGTYELPEAQMDRFLLKEIVEYPSPAEEFEILGRIDSGVLNPD
                     RHVPGVISLEDVQDLQDIASRVYIDPAIRNYIVQLAYVTRNPAPYIGEERARFIKYGA
                     SPRASIAFLQASRALALLNGRTHVLPEDIRALRHLVLRHRVLLTFEADAEGIRSEEII
                     DQIFASVPTP"
     gene            1814..2752
                     /locus_tag="VN041_11145"
     CDS             1814..2752
                     /locus_tag="VN041_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWU29629.1"
                     /translation="MASLITQVKSKLFIRSSQKSMHALDGAYASLLRGRSLDFEDLRP
                     YEFGDQVRDIDWRATAKQGEPLIKRTKATRMHTVLFLVDSGRGMKALAEDERSKRDLV
                     VLLTGALGFLTVRHGDDFSIIYGDSDRVRRLPPGGTEGALEHALRTIRQAIDASDAPS
                     DRDALLRYTARNISRRSIVVIVTDEAPMSDDTDRLLRRLKVQHDVLWLTVRDAEIVVA
                     GSGRASAFDRAVDPGLARTAPGHRRDVDSGWGIPDFVHGDAEVMAELAALEEADTAHR
                     DEVLRRHEITHAEFGGQDTAVATLLTMLNRRSHARA"
     gene            2739..3227
                     /locus_tag="VN041_11150"
     CDS             2739..3227
                     /locus_tag="VN041_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29630.1"
                     /translation="MPAPDDLYPPVQYGWGWILLAIGILLLLGVAAWLLWFLTRPKHS
                     ITLHGAPSYTPPTADVLTQLRTEYLADVQRIEDAYRSGNMTPKQANLELSRLVRGFVN
                     DYSGLEAPVLALDDLVARGVDPALIDAIRRHYYPSIFQRGPAIDPRYGAEAARKVVQT
                     WH"
BASE COUNT          531 a         1274 c         1047 g          489 t
ORIGIN      
        1 gcgaatccgg gcccgacgaa ccccggtccg acgacgccga ccaaccccgt tccctcggac
       61 ccgaccccgc ccgctccggc gccccctgcg gcgccgaagc cgctggcgtt catcgggatc
      121 acaccgaact acgtcctcaa catcctcaac ctcccgctgc tgacgagcta cacgctctcg
      181 ctgtccggcc agcccggggc gaaggcggcc gtgacctacg gaggcctgcc cgccggaacg
      241 gtcaccttcg acagcggcgg ccgcgcctcc ctcgtcgtag gcgcctccct gctgggtctg
      301 agcaacccga tgatcggcgc ctcgtactcc gacggcaccg ccggagccgc gatccaggcc
      361 cgccgcgact cgatctgaga ccccgcgtcc cctccatccg gagcatgcgc acagacgacg
      421 tcgccgtcgg cggttaccct gtaccggggg gatcagcggt cgtcgtcgat ctctcgacgc
      481 acgaggagaa gacagatgac cgacttcccg gttcccggcg ccgcttcgtc cgcgcccccg
      541 cctccgcccg cgccggctgc accgatcgca tccgcaccga ctgccgcacc gggttccgcc
      601 gatgcgcact gggacacgga tgccgtcgac gctacgcgcc cgtccgcccc gccgcgaccg
      661 ccgctgccca ccacgtcgac ggactcgggt gtccccgtgg cttcggcaga ccgccgcggg
      721 aacgcgactc ctgaggagct cgcccgggcg cagcgcatcc tgaaggtcat ctcggactcg
      781 tactccacca agatggtcgg ccaggaccgg ctgcgcagca gcctgctcat ctcgctgatc
      841 gcgggcgggc acatccttct ggagtccgtg cccggactcg ccaagaccac ggcggcgagc
      901 acgctcgccg acaccgtgaa ggccagcttc aagcgcatcc agtgcacccc cgacctgctg
      961 cccagcgaca tcaccggcag ccagatctac gacgctgcga ccggcacctt ccgcaccgtg
     1021 ctcggaccgg tgcacgccaa cttcgtgctg ctcgacgaga tcaaccgctc cagcgccaag
     1081 acccagtcgg ccatgctcga ggcgatgcag gagcggcaga ccaccatcgg cggcgagatc
     1141 cacgccctgc cgaagccctt cctcgtgatc gcgacgcaga accccatcga gcaggagggc
     1201 acctacgagc tgcccgaggc tcagatggac cgcttcctgc tcaaggagat cgtggagtac
     1261 ccgagcccgg ccgaggagtt cgagatcctc gggcgcatcg actccggagt gctcaacccc
     1321 gaccgccacg tgcccggcgt gatctccctc gaggacgtgc aggatctgca ggacatcgcc
     1381 tcgcgggtct acatcgaccc ggcgatccgc aactacatcg tgcagctcgc gtacgtcacc
     1441 cggaacccgg cgccgtacat cggcgaggag cgcgcccgct tcatcaagta cggcgccagc
     1501 ccccgtgcga gcatcgcgtt cctgcaggcc tcccgtgcgc tggcgctgct gaacggccgc
     1561 acccacgtgc tgcccgagga catccgcgcg ctgcgccacc tggtgctgcg tcaccgcgtg
     1621 ctgctcacct tcgaggcgga tgccgagggg atccgcagcg aggagatcat cgaccagatc
     1681 ttcgcgtcgg tgcccacccc gtaacgacgc ttcgatcccc atgccccact ccagccgccg
     1741 ctccgcgtcg ccaggagcct cgggggcacg gggccccgct cagcaatcta ttgaggacag
     1801 tttcccggca gccatggcaa gtctgatcac ccaggtgaag agcaagctct tcatccgctc
     1861 atcgcagaag tcgatgcacg cgctcgatgg cgcctatgca tcgctgctgc gcgggcgcag
     1921 cctcgacttc gaggatctgc gtccgtacga gttcggcgat caggtgcgcg acatcgactg
     1981 gcgtgccacg gccaagcagg gcgagccgct catcaagcgc accaaggcga cgcggatgca
     2041 caccgtgctg ttcctggtgg attccggtcg cggcatgaaa gcactcgccg aggacgagcg
     2101 ctccaagcgc gatctggtgg tgctgctgac cggcgccctc ggcttcctca ccgttcgcca
     2161 cggagatgac ttctcgatca tctacggcga ttccgaccgc gtgcgccggc tgccccctgg
     2221 cggcaccgag ggcgcgctcg agcacgccct gcgcaccatc cgccaggcca tcgacgccag
     2281 cgacgccccg agcgacagag acgccctgct ccgctacacg gcgcgcaaca tctcgcgacg
     2341 ctccatcgtc gtgatcgtca ccgacgaagc gccgatgtcg gatgacaccg accgtctgct
     2401 gcggcggctc aaggtgcagc acgacgtgct gtggctgacc gttcgggacg ccgagatcgt
     2461 cgtcgccggg agcgggcgcg cgagcgcgtt cgatcgcgcc gtcgaccctg gtctcgcccg
     2521 caccgcaccc ggtcaccgcc gcgacgtgga cagcggctgg ggcatccccg acttcgtgca
     2581 cggcgacgcc gaggtgatgg cggagctggc cgcgctcgag gaggccgaca ccgcgcaccg
     2641 cgacgaggtg ctgcgccgcc acgagatcac gcacgccgag ttcggcgggc aggacaccgc
     2701 ggtggcgacc ctgctgacga tgctgaacag gaggtcgcat gcccgcgcct gacgacctct
     2761 atccgcccgt gcagtacggc tggggctgga tcctgctcgc gatcggcatc ctgctgctgc
     2821 tcggtgtcgc cgcctggctg ctgtggttcc tcacccggcc gaagcacagc atcacgctgc
     2881 acggcgcccc ctcctacaca cctccgacag ctgacgtgct cacgcagctg cgcaccgagt
     2941 acctcgccga cgtgcagcgc atcgaagatg cataccgatc cgggaatatg accccgaaac
     3001 aggcgaacct cgagctgagc cggctcgtgc gcggcttcgt caacgactac tccggcctgg
     3061 aagcccccgt cctcgcgctc gacgacctgg tcgcccgggg cgtcgacccg gcgctgatcg
     3121 acgccatccg ccggcactac taccccagca tcttccagcg cgggccggcg atcgatccgc
     3181 gctacggcgc tgaggctgcg cgcaaggtgg tgcagacatg gcactgaaca cctggtggat
     3241 gatcctcgtc gcgctcggcg tactgctcgt cgccgccggc gtgggcgtgc ggttgcgtgg
     3301 gtagaccagg ctcagtggac tcggcgtcgg tggcgtctcg g
//
LOCUS       DATMSX010000359         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_185378,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000359 DATMSX010000000
VERSION     DATMSX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185378"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VN041_11155"
     CDS             <1..1042
                     /locus_tag="VN041_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWU29631.1"
                     /translation="AAAVAARLLPAATATDTGGSIRQPAHVTGTVGTKPTYGGVSRYG
                     SIALASSLDQVGPVTRTVLDAGLLHDVIGGYDAADSTSLRDEWPSFAAAAREGATGEV
                     LKGLKVGVIKELPDSGFQAGVAASFRESLALMEAQGAEIVEISAPHFEYGVAAYYLIL
                     PAEASSNLAKFDSVRFGLRVTPEGGATVEDVMSATREAGFGPEVKRRIILGTYALSAG
                     YYDAYYGSAQKVRTLIQRDFDQAFAQVDVIATPTAPTTAFKLGEKIDDPLQMYLNDLT
                     TIPANLAGVPGISIPSGLAAEDGLPVGIQFLAPAREDARLYRVGAALEAALVDAWGAP
                     LLEKAPVLGGAR"
     gene            1042..2553
                     /gene="gatB"
                     /locus_tag="VN041_11160"
     CDS             1042..2553
                     /gene="gatB"
                     /locus_tag="VN041_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWU29632.1"
                     /translation="MATVKLMDFDKALELFEPVLGFEVHVELNTRTKMFSAAPNPANE
                     EFHGAEPNTLIAPVDLGLPGSLPVVNATAVRSSISLGLALGCSIAESSRFARKNYFYP
                     DLGKNYQISQYDEPIAFEGSVEVELEDGTLVTIPVERAHMEEDAGKLTHMGSTGRIQG
                     AEYSLVDYNRAGVPLVEIVTKPIFGAEHRAPEYAKAYISAIRDIVRGLGISEARLERG
                     NLRCDANVSLRPRGQEKLGTRTETKNVNSMRSVERAVRYEIQRQAAILADGGTITQET
                     RHWHEDTGTTSPGRPKSDADDYRYFPEPDLLPVQPSRELVEELRAELPEQPVARRRRL
                     KAEWGFTDLEFQDVRNGGLVEVVEATIAAGATPASARKWWTGEITRLANAAEQDASEL
                     ISPENVAALQQLVDAGTLTDKLARQVLEGVIAGEGTPQQVVDARGLAVVSDDGALFAA
                     IDEALAAQPDVMAKIKDGKVQAAGAIIGAVMKAMKGQADAARVRELILERAAQ"
BASE COUNT          419 a          948 c          910 g          369 t
ORIGIN      
        1 ggccgccgcc gtggccgctc gcctgctgcc tgccgccacc gcaaccgaca ccggcggctc
       61 gatccgtcag ccggcccatg tgaccggaac ggtcggcacc aagcccacct acggcggcgt
      121 gagccgctac ggctcgatcg ctctggcctc cagcctggac caggtcgggc cggtcacccg
      181 cacggtgctc gacgcgggcc tgctgcacga cgtcatcggc ggctacgacg cggccgactc
      241 cacgtcgctg cgagacgagt ggccctcgtt cgccgccgcg gcccgtgagg gcgcgaccgg
      301 cgaggtcctc aaggggctga aggtgggcgt gatcaaggag ctgcccgact ccggcttcca
      361 ggccggagtc gccgcgtcct tccgcgagtc gctcgccctg atggaggcgc agggcgccga
      421 gatcgtggag atctccgccc cgcacttcga atacggcgtg gcggcgtact acctgatcct
      481 ccccgcggag gcgtccagca acctggccaa gttcgactcc gtgcgattcg gtctgcgcgt
      541 cacccccgag ggcggcgcga cggtcgagga cgtgatgtcc gccacccgcg aggccggttt
      601 cggtcccgag gtgaagcgtc gcatcatcct cggcacgtac gcgctctccg ccggctacta
      661 cgacgcgtac tacggcagcg cgcagaaggt ccgcacgctg atccagcgcg acttcgacca
      721 ggcgttcgcg caggtcgacg tcatcgccac gcccacggct ccgaccaccg cattcaagct
      781 cggcgagaag atcgacgacc cgctgcagat gtacctgaac gacctgacca cgatcccggc
      841 gaacctcgcc ggcgtccccg gcatctcgat cccgtcgggt ctggccgccg aggacggact
      901 gcccgtcggc atccagttcc tcgcgcccgc ccgggaggat gcccggctgt accgcgtcgg
      961 tgcggcgctc gaggccgcgc tggtggacgc ctggggcgca ccgctgctgg agaaggcacc
     1021 cgtcctggga ggagcacgct gatggccacc gtcaagctga tggacttcga caaggctctc
     1081 gagctcttcg agccggtgct cggcttcgag gtgcacgtcg agctgaacac gcgcacgaag
     1141 atgttctccg cggcgccgaa ccccgccaac gaggagttcc acggtgcaga gccgaacacg
     1201 ctcatcgcac cggtggacct cggtcttccc ggctcgctgc cggtggtcaa cgccaccgcc
     1261 gtgcgctcgt cgatcagtct gggcctcgcg ctcggctgct cgatcgccga gtccagccga
     1321 ttcgcccgga agaactactt ctacccggac ctcggcaaga actaccagat ctcgcagtac
     1381 gacgagccga tcgccttcga gggctcggtc gaggtcgagc tcgaggatgg cacgctggtg
     1441 accatcccgg tcgagcgcgc gcacatggag gaggacgccg gaaagctcac ccacatgggc
     1501 tcgacgggtc gcatccaggg tgccgagtac tcgctggtgg actacaaccg tgcaggcgtg
     1561 ccgctggtgg agatcgtcac caagccgatc ttcggcgccg agcaccgcgc gccggagtac
     1621 gccaaggcgt acatctcggc catccgcgac atcgtccgag gcctgggcat ctccgaggcg
     1681 cggctggagc gcggcaacct gcgctgcgac gccaacgtct cgctccgccc gcgcggccag
     1741 gagaagctcg gcacccgcac cgagacgaag aacgtcaact ccatgcgctc ggtcgagcgc
     1801 gccgtgcgct acgagatcca gcgccaggcg gcgatcctcg ccgacggcgg cacgatcacg
     1861 caggagacgc ggcactggca cgaggacacc ggcaccacct cgcctggtcg tccgaagtcg
     1921 gatgctgatg actaccgcta cttcccggag cccgacctgc ttcccgtgca gccctcgcgc
     1981 gagctggtgg aggagctgcg cgccgagctg ccggagcagc ccgtcgcgcg tcgccgcagg
     2041 ctgaaggccg aatggggctt caccgacctg gagttccagg acgtgcgcaa cggcggactc
     2101 gtggaggtcg tcgaggcgac catcgcggcc ggggcgacgc cggcatccgc tcgaaagtgg
     2161 tggacgggcg agatcacgcg tctcgcgaac gccgccgagc aggacgcgtc cgagctgatc
     2221 agtccggaga acgtcgccgc cctgcagcag ctggtcgacg cgggaaccct gacggacaag
     2281 ctggcccgcc aggtgctcga gggcgtcatc gccggcgagg gcaccccgca gcaggtcgtg
     2341 gatgcccgcg gcctggcggt ggtctccgac gacggagctc tgttcgccgc catcgatgag
     2401 gcgctggccg ctcagcccga cgtgatggcg aagatcaagg acggcaaggt gcaggccgcc
     2461 ggcgcgatca tcggtgcggt gatgaaggcc atgaagggcc aggccgacgc cgcccgcgtg
     2521 cgcgagctga tcctggagcg cgcggcccag tagcggccat ccgggctgct gttcagatcg
     2581 cctgcgcggt ggacagcagc ccggcgctca gcagtcgcgc gaccgcgcgg agactggcgg
     2641 cctcgt
//
LOCUS       DATMSX010000360         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_100250,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000360 DATMSX010000000
VERSION     DATMSX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100250"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VN041_11165"
     CDS             <1..1093
                     /locus_tag="VN041_11165"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWU29633.1"
                     /translation="PAGDRRFTFLGDLRTESGQHLPVMRLAWESWGELNAARDNAVLI
                     LHALTGDSHVRGEAGAGHPTAGWWDSVVGPGAAIDTDRWFVIAPNMLGGCQGSTGPAS
                     IAPDGREWASRFPYLTIRDQVAAQIRLADELGIETWAAVIGGSMGGMHALEWAVMAPE
                     RVERLAVLSSPPVTTADQLALNFVQLETVRMDPRFAGGEYYDAALGEGPHRGLALARR
                     MALLNYRSPIELNQRFQRSWQSGVSPLGHGGRFAVESYLDFHGNKFTRRFDANSYITV
                     VEAMNSHDIGRDRGGVEEALHTVTAKSLVIGIDTDRLFPVDGQQRIARSIPNLIDDEA
                     IVLASDFGHDGFLIETDEVGMHLQRLLDA"
     gene            complement(1127..2350)
                     /locus_tag="VN041_11170"
     CDS             complement(1127..2350)
                     /locus_tag="VN041_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29634.1"
                     /translation="MTPSNLSRGAAMRALLSLAIGSFGIGMTEFVMMGLLPDIAQDLL
                     PDLWARSQEQAIGQSGVLVSMYALGVVIGAPTIAGAVARYPRHRVMLGLAVALTLFNA
                     LVLVLPGFGLVGMARLLAGLPHGAYFGIGALVAADVLGPGRRAQGVAFILTGLTIANV
                     VGVPVGTYLGQQWGWRTAFLAVTLVFALGAVCIALFVPAHEGDPGRTMRSELGVFRSS
                     QVWLTLGIGAIGFGGFFAVYSYIAPLVTEVAGAPQWAVPVTLVLVGVGMTVGNLAGGY
                     FADRDLRATLHLGLAGLAAALVLLALLAWSFAALVVLALIIGALSAMISPAIQTRLMD
                     VAGDNQSIAAALNHSALNIGNSLGAFLGGIAISLGLGFTAPEWVGAGLALLGLGITVI
                     AYRAERRGLQASVTV"
     gene            complement(2424..3074)
                     /locus_tag="VN041_11175"
     CDS             complement(2424..3074)
                     /locus_tag="VN041_11175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWU29635.1"
                     /translation="MSADGEIRVAGTAVVLRDGLEGVETLMLRRPSKGSFAGAWVFPG
                     GRVDPGDWADADDEADAARAAAVRETLEETAIRIADVTLLSRWTPPPEQPVKFRTWFF
                     LARDLGDEVRVNPGEIEEAVWLSPERVFAQHEASSLTLFPPTWVTLNGLLGHRTVDDA
                     LAAVGEPALYATNLRDGVDGRFALWAGDAEHADEPGGPDARHRLLMGTLPWRYERR"
     gene            complement(3076..3393)
                     /locus_tag="VN041_11180"
     CDS             complement(3076..3393)
                     /locus_tag="VN041_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HWU29636.1"
                     /translation="MLVAFSVAPSGNGRSGDSVHDAVAAAVRVVRESGLAHRTTSMFT
                     EIEGPDWDTVMDVVKRATEAVMPFGSRVSLVLKADIRPGYSGELDAKIERLEAAIDAA
                     DQD"
     gene            complement(3421..>4164)
                     /locus_tag="VN041_11185"
     CDS             complement(3421..>4164)
                     /locus_tag="VN041_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29637.1"
                     /translation="GAALLTIGAWVKIWPGAVVVAAIAAGRRSWQVLLSAASVTIVII
                     GVLLMLGAGGNLLGFLGMQTGRGLQIEAVGATPFLWMVVAGAARIEYSYEILTFQITA
                     PGADLVSTLLTPLMVLVVAGILVVGALRARGGAAWQRLLPPLTLALVTGLMVTNKVGS
                     PQFHTWLIPPVILWIVFDRARSRTAGVLVLVLCTLTFAIYPLTYDQLLRAEALPMLLL
                     TARNLLLVVLLVHAVHAVVRVPRGSVTTT"
BASE COUNT          677 a         1486 c         1471 g          530 t
ORIGIN      
        1 ccctgcgggc gaccgccgct tcacgttcct gggcgacctg cgcaccgaga gcggccagca
       61 cctgcccgtg atgcgcctgg cctgggagtc ctggggcgag ctcaacgcgg cgcgcgacaa
      121 cgcggtgctc atcctgcacg cgctgaccgg tgacagccac gtgcgcggcg aagccggcgc
      181 cgggcatccg acggccggct ggtgggattc cgtcgtcggc ccgggtgcgg cgatcgacac
      241 cgatcgctgg ttcgtgatcg ccccgaacat gctcggcgga tgccagggat cgaccggccc
      301 cgcgagcatc gccccggacg gccgggaatg ggcatcccgc ttcccctacc tcaccatccg
      361 cgaccaggtg gcggcgcaga tccgcctggc cgacgaactc ggcatcgaga cctgggccgc
      421 cgtgatcggc ggctcgatgg gcggcatgca cgccctggag tgggctgtca tggctcctga
      481 gcgcgtcgag cgcctggccg tgctctcctc tcccccggtg accacggcag atcagctcgc
      541 cctgaacttc gtgcagctgg agaccgtgcg gatggacccg cgcttcgccg gcggcgagta
      601 ctacgacgcg gcgctcggag agggcccgca ccgcggtctg gcgctggccc gccgcatggc
      661 gctgctgaac taccgcagcc ccatcgagct gaatcagcgg ttccagcgct cctggcagtc
      721 cggggtctcc ccgctgggcc acggcggccg cttcgcggtg gagtcgtacc tggacttcca
      781 cggcaacaag ttcacccgcc gcttcgacgc gaacagctac atcaccgtgg tcgaggcgat
      841 gaactcccac gacatcgggc gcgaccgcgg cggtgtcgag gaggcgctgc acaccgtcac
      901 cgcgaagagc ctggtgatcg gcatcgacac cgaccggctg ttccccgtcg acgggcagca
      961 gcgcatcgcg cgcagcatcc cgaacctcat cgacgacgag gcgatcgtgc tggccagcga
     1021 cttcggccac gacggcttcc tcatcgagac cgacgaggtc gggatgcatc tgcagcggct
     1081 gctcgacgcc tgagccatcc cgcagtaacc gccggcgatc cgcggctcag accgtgaccg
     1141 aggcctgcaa cccgcgtcgc tccgcccggt acgcgatcac ggtgatgccg agccccagca
     1201 gcgccagccc tgcgcccacc cactccggag cggtgaagcc cagcccgagc gatatcgcga
     1261 tgccgccgag gaacgcgccc aggctgttgc cgatgttcag cgcggagtgg ttcagcgcag
     1321 cggcgatcga ctggttgtct ccggcgacgt ccatcagccg ggtctggatg gccgggctga
     1381 tcatcgccga gagcgcgccg atgatcagcg ccaggaccac caacgcggcg aacgaccagg
     1441 ccagcagcgc gaggaggacg agcgccgcgg cgaggcccgc caggccgagg tgcagcgtcg
     1501 cgcgcaggtc gcggtccgcg aagtagccgc ctgcgaggtt gccgaccgtc atgcccacac
     1561 ccaccagcac cagcgtcacg gggacggccc actgcggtgc tccggcgacc tcggtgacga
     1621 gcggggcgat gtagctgtac accgcgaaga agccgccgaa cccgatcgcg ccgatgccca
     1681 gcgtcagcca tacctgcgag gagcggaaca cgcccagctc ggagcgcatc gtgcggccgg
     1741 gatcgccctc atgggcgggc acgaacaggg cgatgcacac cgcgccgagc gcgaacacca
     1801 gggtgacggc gaggaacgcg gtccgccagc cccactgctg gccgagatag gtgccgacgg
     1861 gcacgccgac gacgttcgcg atcgtgagtc cggtgaggat gaaggccacg ccctgagcac
     1921 gacgtcccgg gcccagcacg tcggccgcga cgagcgcgcc gatgccgaag taggcgccgt
     1981 gcggaaggcc agccagcagg cgcgccatcc cgaccagccc gaagccgggc agcaccaaga
     2041 cgagggcgtt gaacagggtg agggcgacgg cgaggccgag catcacccga tgacgcggat
     2101 accgcgcgac cgcaccggcg atggtcggcg cgccgatcac gacgcccagc gcgtacatcg
     2161 acacgagcac gccgctctgg ccgatcgcct gctcctggct gcgcgcccag aggtcgggca
     2221 gcaggtcctg ggcgatgtcg ggaaggaggc ccatcatgac gaattcggtc atgccgatgc
     2281 cgaagctgcc gatggcgagg gagaggagcg ccctcatcgc cgcacccctc gagaggttcg
     2341 agggagtcac ccgtccatgc tagcggcgcg cgagtgcggc gatgacggga tgacgagctg
     2401 gtgccgcgtc agcggatgcc gcatcagcgg cgctcgtacc gccagggcag tgtgcccatc
     2461 aggagccggt gccgggcgtc cggtcccccg ggctcatccg cgtgctcggc gtctcccgcc
     2521 cacagcgcga agcggccgtc gacgccgtcg cgaaggttcg tggcgtagag cgccggttcg
     2581 ccgacggcgg cgagcgcgtc atcgaccgtc cggtgcccga gcagtccgtt cagggtcacc
     2641 caggtcggcg ggaagagggt caggctgctc gcctcgtgct gcgcgaacac ccgctcgggg
     2701 ctcagccaca ccgcctcctc gatctccccg gggttcaccc gcacctcgtc gccgaggtcg
     2761 cgagcgagga agaaccaggt acggaacttc acgggctgct cgggcggcgg cgtccagcgc
     2821 gagagcaggg tgacatcggc gatgcggatc gcggtctcct ccagggtctc ccgcacggcc
     2881 gcggcgcggg cggcatccgc ctcgtcgtcc gcatccgccc agtcgcccgg atcgacgcgt
     2941 ccaccgggga acacccaggc tcccgcgaag gatcccttcg acgggcgccg cagcatcagc
     3001 gtctccacgc cctccagtcc atcgcggagc acgacggcgg tgccggcgac gcggatctcc
     3061 ccgtcggcgc tcatctcagt cctggtcggc ggcgtcgatc gccgcttcga gccgctcgat
     3121 cttggcatcc agctccccgc tgtagccggg acggatgtcg gccttcagca ccagtgagac
     3181 gcgggagccg aacggcatca ctgcctcggt ggcgcgcttg acgacatcca tcacggtgtc
     3241 ccagtccggg ccctcgatct cggtgaacat gctcgtggtg cggtgcgcga gaccggactc
     3301 gcgaacgacg cggaccgcgg cggcgaccgc gtcgtggacg gagtcgccgg agcggccgtt
     3361 gccggacggg gcgactgaga aggcgacgag catgggtgtc ctccgatttc aggggtgact
     3421 tcaggtggtg gtcacggatc cgcgcggtac gcgcacgacg gcgtgcaccg catggacgag
     3481 caggacgacc aggagcaggt tgcgcgcggt gagcagcagc atgggcagcg cttcggcgcg
     3541 cagcagctgg tcgtaggtga gggggtagat cgcgaaggtc agcgtgcaga gcaccaggac
     3601 gagcaccccc gcggtgcgcg atcgggcgcg gtcgaagacg atccacagga tgaccggcgg
     3661 gatcagccag gtgtggaact gcggagagcc gaccttgttg gtcaccatca gccccgtcac
     3721 gagcgccagc gtcagcggcg gcaggagccg ctgccacgcc gcgccgccac gcgcgcgaag
     3781 agcgccgacg accagaatcc cggcgacgac gagcaccatc agcggcgtca gcagcgtgga
     3841 gacgaggtcc gccccgggcg cggtgatctg gaaggtcagg atctcatagc tgtactcgat
     3901 gcgcgcggcg ccggccacga ccatccacag gaacggcgtg gcccccacgg cctcgatctg
     3961 caggccgcgg ccggtctgca tgccgaggaa tcccagcagg ttcccgcctg cgcccagcat
     4021 cagcagcacg ccgatgatca cgatcgtgac ggatgctgcg ctcagcagca cctgccagga
     4081 ccgtcggccg gcggcgatcg cggcgaccac caccgcgccg ggccagatct tcacccacgc
     4141 gccgatcgtg agcagggcgg cgcc
//
LOCUS       DATMSX010000361         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_100283,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000361 DATMSX010000000
VERSION     DATMSX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100283"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VN041_11190"
     CDS             <1..372
                     /locus_tag="VN041_11190"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWU29638.1"
                     /translation="EAALAVIDDALALQEAGCALIVVEAVPSEVTGLLVERLLIPVIG
                     IGAGADADGQVLVWHDLLGIYDGPAAKFVKRYADVRAASVAGVDEYAREVRSGAYPAP
                     EHGYGMPADEVVKLKELLAGR"
     gene            complement(377..826)
                     /locus_tag="VN041_11195"
     CDS             complement(377..826)
                     /locus_tag="VN041_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29639.1"
                     /translation="MSQAAEQIDLETSIGYALKEAASALRLAMEAVLRPLGLTITEYS
                     CLELLAQHPGLSNSDLARGAFVTRQSMSVLLQALERDGLVSRPAEAPVGKVLPTRLTP
                     QGRTSLDAASAAVRAVELRMLSGMTADEQAVALAGLQSMIRSLRTDE"
     gene            889..1257
                     /locus_tag="VN041_11200"
     CDS             889..1257
                     /locus_tag="VN041_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU29640.1"
                     /translation="MSVTGPDFISLQVRDLAASQAFYERYLGMVRSQAGPPHAVVFET
                     TPIAFALRDIVPGTDLASAPYPGIGAAVWLHATDVQAIHDALVADGHSIVAAPIDGPF
                     GRTFTFADPDGYRITLHDRA"
     gene            complement(1390..3600)
                     /locus_tag="VN041_11205"
     CDS             complement(1390..3600)
                     /locus_tag="VN041_11205"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWU29641.1"
                     /translation="MPDTTLTAETTYLDRADLRVAREIVDFAEPSLADAGLDADAFWT
                     GVSSIVHDLGPRNAALLEKRAMLQARIDEYHRANPGRPEPEAYRAFLTEIGYLVPEPA
                     PFAITTENVDPEIATMAGPQLVVPLLNARFALNAANARWGSLYDALYGTDAIDQTGEL
                     APGRDYNPARGAEVIAHGRALLDQAAPLSEGSHTEATAYRVDAEGLVVESATGIRRLA
                     DPAAFVGHTGDADAPTAILLQHHGLHLEIVIDPEGAIGRTDAAGVQDIVLESAITTIV
                     DLEDSVAAVDAEDKALGYANWRGLMDGTLSEEVSKGGRTFTRRLAADRTYTGRDGAEL
                     TLPGRSVLFIRNVGHLMRTDAVLDRDGEEVYEGILDAIMTALGALPELTGPNRGANSR
                     TGSDGAGSMYIVKPKMHGPEEAAFAVELFGRVEQLLGLPERTLKVGIMDEERRTSANL
                     AASIAAASDRVVFINTGFLDRTGDEIHTSLHAGPFLPKGGIKSQPFMQTYEDRNVRIG
                     LAAGLGGRAQIGKGMWAMPDLMHDMLEQKIGHVRSGASTAWVPSPTAATLHALHYHQV
                     DPFAVRPTLPAYDPASLDTLLQPPLASDGDLTPEIVATELDNNVQSILGYVVRWIDQG
                     VGCSKVPDIHGIALMEDRATLRISSQLLANWLQHGVITVDQVDAALQRLASVVDEQNA
                     GDAAYEPLAFGSDAEPGIAYTAARRLILEGATQPSGYTEPLLHALRRAKKAQQG"
BASE COUNT          520 a         1287 c         1358 g          634 t
ORIGIN      
        1 gaggcggcgc tcgccgtgat cgacgacgcc ctcgccctgc aggaggcggg ctgcgcgctg
       61 atcgtcgttg aggcggtgcc ttcggaggtg acggggctgc tcgtggagcg cctgctgatt
      121 cccgtgatcg ggatcggcgc cggagcggat gccgacggcc aggtgctcgt gtggcacgac
      181 ctgctcggca tctacgacgg gccggcggcg aagttcgtca agcggtacgc cgacgtgcgc
      241 gcggcctcgg tcgccggtgt cgacgagtac gcccgcgagg tgcggtcggg tgcgtacccg
      301 gcgccggagc acgggtacgg gatgcccgcg gacgaggtcg tgaagctgaa ggagctgctc
      361 gccggacggt gagcccctac tcgtcggtgc gcagcgagcg gatcatgctc tgcaggccgg
      421 cgagcgcgac cgcctgctcg tctgccgtca ttccgctcag cattcgaagt tccactgccc
      481 gcacggcggc gctcgcggcg tcgagcgacg tgcggccctg gggtgtgagc cgcgtcggca
      541 gcaccttccc gaccggcgcc tcggcgggtc tgctcacgag cccgtcccgc tcgagagcct
      601 gcagcagcac gctcatcgac tgccgcgtga cgaaggcgcc gcgggcgagg tcggagttcg
      661 acaacccggg atgctgggcg agaagctcca gacaggaata ttcggtgatg gtcagcccga
      721 gcggtcgcag cacggcctcc atcgccagtc gcagcgcgct tgcagcctct ttgagcgcat
      781 aaccgatgga cgtctccagg tcgatctgtt ctgccgcttg actcatgtca gtattctgac
      841 atacgttgga tcatgtcaga taactgacat gaaactgagg agcacagcat gtccgtcacc
      901 ggacccgact tcatctctct gcaggtgcgc gatctcgcgg cgtcgcaggc gttctacgag
      961 cgttacctcg gaatggtccg ctcgcaggct ggaccgcccc acgccgtcgt cttcgagacg
     1021 accccgatcg cattcgcgct gcgcgacatc gtgccgggca cggatctggc atccgctccg
     1081 tacccgggaa tcggcgcggc ggtctggctg cacgccaccg acgtgcaggc gatccacgac
     1141 gcgctggtcg ctgacggtca ctcgatcgtc gccgctccga tcgatgggcc cttcggccga
     1201 accttcacct tcgccgaccc ggatggatac cggataaccc tgcacgaccg cgcctgaggt
     1261 cgggctcgac atccgtccgg gtgcagatgt cggtcgagat cacggatatc ggctcagaag
     1321 tggcatccgg gctgacatcc gtactttggg ccgacatccg taccggacgg cgcgccggat
     1381 caggccggat cagccctgct gggccttctt cgcccgccgc agcgcgtgca gcagcggctc
     1441 ggtgtagccg ctgggctgcg tcgcaccctc caggatcagg cgccgcgccg cggtgtacgc
     1501 gatgcccggc tcggcatccg atccgaacgc gagcggctcg tacgccgcgt cgccggcgtt
     1561 ctgctcatcc accacggatg cgaggcgctg cagcgcagca tccacctggt cgaccgtgat
     1621 cacgccgtgc tgcagccagt tcgcgagcag ctggctcgag atgcgcaggg tggcgcgatc
     1681 ctccatgagt gcgatgccgt ggatgtcggg caccttcgag catccgacgc cctgatcgat
     1741 ccagcgcacc acgtagccga ggatcgactg cacgttgttg tcgagctcgg tcgcgacgat
     1801 ctcgggcgtg aggtcgccgt cggaagccag cggcggctgc agcagcgtgt ccagcgacgc
     1861 cgggtcgtac gccggcagcg tcggccgcac ggcgaacgga tcgacctggt ggtagtgcag
     1921 ggcgtgcagg gtcgccgcgg tcggggacgg cacccaggcg gtggatgccc cggagcgcac
     1981 gtgcccgatc ttctgctcga gcatgtcgtg catcaggtcg ggcatggccc acatgccctt
     2041 gccgatctgg gcgcgcccgc cgagccccgc ggccaggccg atgcgcacgt tgcgatcctc
     2101 gtaggtctgc atgaacggct ggctcttgat gccgccctta ggcaggaagg ggccggcgtg
     2161 cagcgaggtg tggatctcgt cgcctgtgcg atcgaggaac ccggtgttga tgaacacgac
     2221 ccgatcggat gctgcagcga tcgacgccgc gaggttcgcc gaggtgcggc gctcctcgtc
     2281 catgatcccg accttgagcg tgcgctccgg cagcccgagc agctgctcga cccgtccgaa
     2341 cagctcgacg gcgaacgccg cctcctccgg tccgtgcatc ttcggcttca cgatgtacat
     2401 cgagcccgcg ccgtctgaac ccgtgcggct gttcgcgccg cggttcggtc cggtcagctc
     2461 gggcagggcg ccgagcgcgg tcatgatggc gtccaggatg ccctcgtaca cctcctcgcc
     2521 gtcgcggtcg agcacggcat ccgtgcgcat caggtggccg acgttgcgga tgaacagcac
     2581 cgagcggccc ggcagtgtga gctcggcgcc gtcgcggccc gtgtaggtgc ggtcggctgc
     2641 caggcggcgg gtgaacgtgc gcccgccctt gctgacctcc tcgctgaggg tgccgtccat
     2701 cagcccgcgc cagttggcgt agccgagcgc cttgtcctcg gcatccaccg cggcgaccga
     2761 gtcctccagg tcgacgatcg tggtgatcgc cgactccagc acgatgtcct gcacgccggc
     2821 ggcatccgtt cgcccgatcg cgccctcggg gtcgatgacg atctcgaggt gcaggccgtg
     2881 gtgctgcagc aggatcgcgg tcggggcgtc ggcgtcgccg gtgtggccga cgaatgccgc
     2941 cgggtcggcg aggcgacgga tgcccgtggc cgactccacc acgaggccct cggcgtccac
     3001 gcgataggcg gtggcctcgg tgtgcgaccc ctccgagagg ggagcagcct ggtcgagcag
     3061 cgcccggccg tgggcgatga cctccgcgcc gcgggccggg ttgtagtccc ggccgggggc
     3121 gagctctccg gtctggtcga tggcatccgt gccgtacagc gcgtcgtaca gcgaacccca
     3181 gcgggcgttc gcggcgttca gcgcgaagcg ggcgttcagc agcggcacca ccagctgcgg
     3241 gccggccatc gtggcgatct cggggtcgac gttctcggtg gtgatcgcga acggtgcggg
     3301 ctcggggacc aggtagccga tctccgtgag gaatgcccgg tacgcctccg gttcggggcg
     3361 accggggttc gcgcggtggt actcgtcgat gcgagcctgc agcatcgcgc gcttctccag
     3421 gagggcggcg ttgcgcggcc cgaggtcgtg cacgatgctc gagacgccgg tccagaacgc
     3481 gtcggcgtcg agcccggcat ccgccagtga tggctcggcg aagtcgacga tctcgcgcgc
     3541 gacccgcagg tcggcacggt cgaggtaagt ggtctcggcg gtgagggtgg tgtcgggcac
     3601 ggtgccgctc cttcggtctg ggggctcgct gtccacggta gagacctcgt tgagtttcgg
     3661 agctttgatt ccgtatggtg aaagccatgc cagctgcctc ctccggttcc gtccagtccg
     3721 tcgcgcgcgc cttctcgctg ctcgaagcgc tcgccgcggc cggcggcgag gcggcgctgg
     3781 catccctggc gtccgcgac
//
LOCUS       DATMSX010000362         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_43704,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000362 DATMSX010000000
VERSION     DATMSX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43704"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..815)
                     /gene="nagB"
                     /locus_tag="VN041_11210"
     CDS             complement(36..815)
                     /gene="nagB"
                     /locus_tag="VN041_11210"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWU29642.1"
                     /translation="MAEVVITASKEAAGALVADEIVHLIDTVAEPVLGLATGSTPLPV
                     YQALRERLAGHDVSHVRGFALDEYVGIDPAHPESYRSVITREVIEPLGLDPELIHVPN
                     GAVETIEHAGDDYDAAIVAAGGVDLQILGIGTDGHIGFNEPGSSFASTTRIKTLTEQT
                     RQDNARFFDSIHDVPQHCITQGLGTILRARHLVLLAFGEGKADAVAAALEGPVSAMIP
                     ASAIQLHPHVTVVVDEAAASKLQLADYYRYTYANKPAWQGL"
     gene            complement(808..1758)
                     /locus_tag="VN041_11215"
     CDS             complement(808..1758)
                     /locus_tag="VN041_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU29643.1"
                     /translation="MSTAVRIGLDVGGTKIAGIALDADGVALGSVRIPTGWGGTAVID
                     GILHAVSALQAQLPAGRLVTSIGVGIPGLVDAEHGRVLHAVNLGVEELDVATRVHEAT
                     GIPSQVENDVKAAALGAAALRGDTRPMAYLNLGTGVAAGIVIDGRIWRGARGTAGEVG
                     HLVVDPHGRVCGCGQRGCIETLCGGGALARAWGRGGDLPVKDILDAADAGDATASALR
                     LDLQRGAAAAVRALVLTADVETVVIGGGLSALADRIGPGIRATLQEEAAASPFLRSLR
                     LDERIEMLPADSPVAAIGAALLGSASAGTDVRDKETVPHG"
     gene            complement(1755..>2076)
                     /locus_tag="VN041_11220"
     CDS             complement(1755..>2076)
                     /locus_tag="VN041_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29644.1"
                     /translation="GKAKKVDALDLPVLVERYSTIALIAFIVVAVSGIARTVVALDLA
                     EVVIAGPADLFDGVFLDAATRALHDRTLEGVFADVPIRLSDQRDIVVRGAVVTVLAAQ
                     LGVS"
BASE COUNT          296 a          746 c          737 g          297 t
ORIGIN      
        1 gcccgccgcc tcgcggcgag ccgagcgcgc agggatcaga gtccctgcca ggccggcttg
       61 ttcgcgtagg tgtagcggta gtagtcggcc agctgcagct tcgacgcggc cgcctcgtcg
      121 accaccacgg tcacgtgcgg gtgcagctgg atcgcggatg ccgggatcat cgcgctgacc
      181 gggccctcga gcgccgcagc caccgcatcc gccttgccct cgccgaacgc gagcagcacg
      241 agatgacgcg cacgcaggat cgtcccgagc ccctgagtga tgcagtgctg cggcacgtcg
      301 tggatcgagt cgaagaagcg cgcgttgtcc tgtcgcgtct gctcggtgag cgtcttgatg
      361 cgggtggtgg acgcgaacga cgagccgggc tcgttgaagc cgatgtggcc gtcggtgccg
      421 atgccgagga tctgcaggtc cacgccgccc gcggcgacga tggccgcgtc gtagtcgtcg
      481 cccgcgtgct cgatcgtctc gacggccccg ttggggacgt ggatcagctc ggggtcgagg
      541 ccgagcggct cgatgacctc acgggtgatg accgagcggt agctctccgg atgcgcggga
      601 tcgatgccca cgtactcgtc cagggcgaag ccgcgcacgt gggacacgtc atgccccgcg
      661 agacgctcgc gcagcgcctg gtagaccggc agcggagtcg acccggtggc cagtcccagc
      721 acgggctcgg cgacggtgtc gatgaggtgc acgatctcgt cggcgaccag cgctccggcg
      781 gcctccttgc tcgcggtgat gacgacttca gccatgaggg actgtctcct tgtctcggac
      841 gtcggtaccc gcggatgcgg atcccagcag ggcggcgccg atggccgcca ccggcgagtc
      901 cgccggaagc atctcgattc tctcatcgag gcgcagcgac cgcaggaacg gcgacgccgc
      961 cgcctcctcc tgcagggtgg cgcggatgcc agggccgatg cggtcggcga gtgcgctgag
     1021 ccctccgccg atgacgacgg tctcgacgtc ggcggtgagc accagggcgc gcacggcggc
     1081 tgcggcaccg cgctgcagat cgagtcggag ggcggatgct gtggcatccc cggcatccgc
     1141 ggcatccagg atgtccttca cggggaggtc tccgcctctc ccccaggccc gcgccagggc
     1201 gccgcctccg cacagcgtct cgatgcagcc gcgttgcccg catccgcaca cccgtccgtg
     1261 cgggtcgacc acgagatgcc cgacctcgcc ggcggtcccc cgtgcgccgc gccagatgcg
     1321 cccgtcgatc acgattcccg cggcgacgcc ggtgccgagg ttcagatagg ccatcgggcg
     1381 ggtgtcgccg cgcagcgcgg ccgctcccag cgcagccgcc ttcacgtcgt tctcgacctg
     1441 gctcgggatg ccggtcgcct cgtgcacgcg ggttgcgacg tccagttcct cgacgccgag
     1501 gttcacggcg tgcagcaccc gtccgtgctc ggcatccacg agccccggga tcccgacgcc
     1561 gatcgaggtc accaacctgc ccgcgggcag ctgcgcctgc agggcgctga cagcatgcag
     1621 gatgccgtcg atgacggcgg tcccgcccca tccggtcggg atgcgcacgc tcccgagggc
     1681 gacgccgtcg gcatccagcg cgatcccggc gatcttcgtg ccgcccacgt ccaggccgat
     1741 gcgtacggcc gtgctcacga cacccccagc tgggcggcga gcaccgtgac gaccgcgccg
     1801 cggaccacga tgtcgcgctg gtcgctgaga cggatgggca cgtcggcgaa cacaccctcc
     1861 agcgtgcggt cgtgcagcgc ccgggtcgcg gcatccagga agacgccgtc gaacagatcg
     1921 gcggggccgg cgatgaccac ctcggccagg tcgagcgcca cgacggtgcg ggcgatgccc
     1981 gagacggcga cgacgatgaa ggcgatcagc gcgatcgtgg agtaccgctc gaccaggacc
     2041 ggcagatcca gggcatccac cttcttcgcc ttgccg
//
LOCUS       DATMSX010000363         9972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_109865,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000363 DATMSX010000000
VERSION     DATMSX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109865"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VN041_11225"
     CDS             <1..854
                     /locus_tag="VN041_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU29645.1"
                     /translation="AFAHARGWPIPIGGSQAISDAMVDDLRAHGGELELEHEVASLAE
                     LPPSTVTLLDVTPKALVRLAGDAMPGRYRRSLEAFRYGDGVAKVDFALNAPVPWTNPD
                     LHLAGTVHVGGTRAEMADAENQVQQGIHPEHPYVLVSQPSLFDTGRAPAGMHTLWTYT
                     HVPAGSDVDRREAVIAQIERFAPGFRDTIVATSSRSAREVEEHNPNYPGGDIAAGAPD
                     LWQLFRRPVLSPDPWRTPIPGVYLASASASPGPGVTGMPGWLAALSALRHEFGTRMLP
                     DLSPESA"
     gene            879..2168
                     /locus_tag="VN041_11230"
     CDS             879..2168
                     /locus_tag="VN041_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29646.1"
                     /translation="MRDDPHGAGTGVRPREVLRIRPFALFWSATTLRSFGGAIAGVAF
                     QVLIVAIVAASPAEISVLSALSVVPYLFLGLIIGALMDRWSRQRTLVLTSIGRAVVLA
                     VIPVLLLFDALTFWSLAAVMLALGVLTLFADSAAQPLLPSLVPRTSLVQANARLGQSE
                     TVAGTAGPALGGALLSLVGAPILFIFEAVIHACAAVLQARIPVQEASPPPRAPGRHIG
                     HEIAEGMRYTYRHRTLRPLAISVHIWFLGNSLVATLFAVYVMRELKVAPWAFGVALAF
                     GGIGGLVGALLAPVLGARLGAGRAILLGRALVVIPWAALALIPADALGTVLVAVVVIS
                     AVQFVYGLSMGIEDANDSGYRQAAAPDAIQGRMNSTIRTMNRIIFFFGALIAGGLATF
                     TGYSITFAIAACVFALAAAVVVFSPLRDARHETARVE"
     gene            2378..3496
                     /locus_tag="VN041_11235"
     CDS             2378..3496
                     /locus_tag="VN041_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29647.1"
                     /translation="MSHARYLTSDHLHRALSLRDLTDPAQGDHAIQMLLDSVVNTLRS
                     GWHSTVRHVRSTPIVPVRENYDRLRYHSGDVTRDRRYTRYVSRTVMLRSHTSAELPTA
                     LEDYAGRTGIDELIVAPGLVYRRDAVDRSHVGEPHQVDLWRIRSAADTDDSDMLVMIE
                     RLVEAVLPGARWRVTDAVHPYTVGGRQIDVLHNGEWLELAECGRIHPDILSGSGLDPA
                     RWSGLALGMGLERALMLRKGIPDIRYLRAKEPRIACQMLTLEPWQHVSPLPATRRDIS
                     VVLDADEDEETVGDRIRIALGDDADVIESVEVLSRTAHEELAEAARARLETQEGQVNL
                     LLRIVLRPIDRTLTADQANAIRNAIYRAVHEGPRMELI"
     gene            complement(3565..3978)
                     /locus_tag="VN041_11240"
     CDS             complement(3565..3978)
                     /locus_tag="VN041_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU29648.1"
                     /translation="MKAHVSSILLGVRDMDRAKKFYTEGLGWKVQSDFGISVFFESDG
                     ASPVGFYGRDGLAAQVGTDPDGNGFSGVVLTYVVRSEARVEEVIAEAQAAGATVLNPA
                     GALPWGGYGGTFADPDGYIWSIGYSDHAPDQPYAE"
     gene            4128..5039
                     /locus_tag="VN041_11245"
     CDS             4128..5039
                     /locus_tag="VN041_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU29649.1"
                     /translation="MELRDIEIFLTLAEELHFGRTATRLHVSQARVSQVIAQLEMQIG
                     GSLFDRSNRRDVRLTPVGTRLRDDLIPVHAALQGSLDRAKLSARGIDARLRVGLMPYN
                     VPVLHGYWDAFAQRHPRYELQVRHASFTDPFGSLRRGEIDILVTWLPVDEADLTVGPT
                     LFTDSRVLAVSVDHELAGRSSVPVEMLADFAHVRVPNMPEAWENGYLPFRTPSGRTIE
                     RVESVTNADDLLQLVSTGEIIHPFPAHVAHYWSLPQIRWVPIPDLPRLTYALVWRTDA
                     ENAPIRAFARMLAELGPVAEGHERRAD"
     gene            5142..5618
                     /locus_tag="VN041_11250"
     CDS             5142..5618
                     /locus_tag="VN041_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU29650.1"
                     /translation="MTTTLTSTPRMPSPAAILDGASGAIFALMKAARQTDLAHSTLEL
                     VHLRVSQINGCSPCVDAGVKSAKQAGESDERLHAVVAWRETPYFTDQERAALSLAESA
                     TRLADRSDAVPDEVWNEAASFFDERQLAALILYIATTNLFNRLNATTRQFAGGSWN"
     gene            5683..5919
                     /locus_tag="VN041_11255"
     CDS             5683..5919
                     /locus_tag="VN041_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29651.1"
                     /translation="MLERRTSLIDGIRSANPAFAEATLIKLDDGSYLDIWRWDSAAEM
                     QRALQNAGSIPLVGATLSLTTDHTVIDGEVLDQR"
     gene            6065..6499
                     /locus_tag="VN041_11260"
     CDS             6065..6499
                     /locus_tag="VN041_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29652.1"
                     /translation="MSNSGDSTGPSPSAAAEALSSADATAGRIRSRRRWYIAGTLVMA
                     AALATFSIALASWPDRLADVIIPGLLIVLAILALLAWSGRTIPEAATKATNRVVLISA
                     VMMIVTLLLNRLLPVGFSGWVVLTGLLPALPFLYLAWRVARA"
     gene            6496..6816
                     /locus_tag="VN041_11265"
     CDS             6496..6816
                     /locus_tag="VN041_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU29653.1"
                     /translation="MTHPRHRLDDVIHSPVRLSVMAALRHADAVHFGLLRDTVEVSDS
                     LLSKHLASLEAAGYVEVTKGYAGRRPRTWYSLTTEGREAFDAYITALREIVDAPESPS
                     GAGS"
     gene            6933..7730
                     /locus_tag="VN041_11270"
     CDS             6933..7730
                     /locus_tag="VN041_11270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29654.1"
                     /translation="MLSVLDRWWANLKGDDGARERALLLPRLYFQHFATTSFVVEDEH
                     GQLAAFLVGFLSQTESDAAYIHFVGVDPLRQGQGVGRALYRAFIDVAGANGRHVVRSV
                     TSPENDGSRAFHRSLGFSASEVIPDYDGPGLARVAFRTTTAAARSRGVRDLRVLDGAL
                     VLERLDGIDVPAGDWVGLVRAPDGLTAIRPAPGSGAKERWIALYEADPDHGLDEPGLL
                     VAVLTPLARSGVPVFVASTYRADMVLVPEDRAQTALTALRSSGFTIS"
     gene            complement(7747..8430)
                     /locus_tag="VN041_11275"
     CDS             complement(7747..8430)
                     /locus_tag="VN041_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29655.1"
                     /translation="MTSPSLEQLAQALAEPRRLQILQELLGGIPLPVGALAARIGIAP
                     STTTAHVTRLLDAGLIAVEQQGRTRLVRIVDPAVAEAVESVLRLTGQPEVLSWAASDR
                     RAAMRRARSCYDHLAGDLGIAVADHLVERGLLDQDLGSARVAVDEIGSALDLSLTVVV
                     SSRPLVRGCLDWTARRPHLAGRLGAALLQAMLTAGWVTRRAEDRSLRITPVGAGHLDR
                     LQLRPSAAG"
     gene            complement(8427..8783)
                     /locus_tag="VN041_11280"
     CDS             complement(8427..8783)
                     /locus_tag="VN041_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29656.1"
                     /translation="MNDLVIAVDDVAADVSRIGAVLGAAGVGLEGGGVWAGQAHYLVA
                     DGAVAVAALDAAGIRATSAPALVVPLRADMPGEMGRMMSALHSAGIRVDAQYSDHDNR
                     KVFVVDDDERARTVLG"
     gene            complement(8780..9232)
                     /locus_tag="VN041_11285"
     CDS             complement(8780..9232)
                     /locus_tag="VN041_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWU29657.1"
                     /translation="MTVHQYSTALSWEGSTGAGYRAYGREHDVQISEEILTLSADAAF
                     RGDASLPNPEQLLLAAASSCLLLSFLAVAAQAGVDVVRYDDDATAFMPMGERPARITR
                     IELAPRVAVRDVEAARVVELLHEAHAGCYIANSLTAEATLTPIVEVLS"
     gene            complement(9393..9483)
                     /locus_tag="VN041_11290"
     tRNA            complement(9393..9483)
                     /locus_tag="VN041_11290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9447..9449),aa:Ser,seq:cga)
     gene            9580..>9972
                     /locus_tag="VN041_11295"
     CDS             9580..>9972
                     /locus_tag="VN041_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWU29658.1"
                     /translation="MPDVIADLTLVGAQSIAERVSMTAAVAELQRILRAGFDPEGDLP
                     RGILDVPNGQLLLMPAAVDGAVGQKFATVAPGNPALGLERIQAVYILLDGTTLAPTAI
                     LDGTALTSLRTPAVSIASVDLLAQPDAGE"
BASE COUNT         1569 a         3469 c         3285 g         1649 t
ORIGIN      
        1 ccgccttcgc gcacgcgcgc ggctggccga tcccgatcgg cggcagtcag gcgatctcgg
       61 atgccatggt cgacgacctc cgcgcgcacg gcggcgagct cgagctcgag catgaggtcg
      121 cctcgctcgc cgagctgccg ccctcgaccg tcacgctgct ggatgtgacg cccaaggccc
      181 ttgtccgcct ggccggtgat gcgatgccgg gtcggtaccg ccgatcgctg gaggcgttcc
      241 gctacggcga cggcgtcgcg aaggtcgact tcgccctgaa cgctccggtg ccctggacga
      301 atccggacct gcacctcgcc ggcacggtgc acgtcggcgg cactcgggca gagatggcgg
      361 atgccgagaa ccaggtgcag cagggcatcc atcccgagca tccgtacgtc ctcgtctcac
      421 agccgtcgct gttcgacacc ggccgcgcac cggcggggat gcacaccctc tggacctaca
      481 cgcacgtccc cgcgggcagc gacgtggatc ggcgggaggc agtgatcgcg cagattgagc
      541 ggttcgctcc gggcttccgc gacacgatcg tcgccaccag ctcgcgcagc gcgcgcgaag
      601 tcgaggagca caacccgaac tacccgggtg gggacatcgc cgccggcgcc cccgacctgt
      661 ggcagctgtt ccgccgaccg gtgctcagcc ccgacccgtg gcgcacgccc atcccgggcg
      721 tgtacctcgc gtcggcgtcg gcgtcgccgg gaccgggtgt caccgggatg cccggatggc
      781 tcgccgcgct gagtgcgctg cggcacgagt tcggcacgcg gatgctgccg gacctgtcgc
      841 cggagagcgc gtgagaccgc acccctagac tgggcgccat gcgcgacgat ccgcacgggg
      901 caggaaccgg tgttcgtccg cgcgaagtgc tccgcatccg gccgttcgcc ctgttctgga
      961 gcgccacgac gctgcgatcc ttcggcggag cgatcgccgg cgtcgccttc caggtgctga
     1021 tcgtcgcgat cgtcgccgcc tcaccggcgg agatcagcgt gctgagtgca ctgagcgtgg
     1081 tcccctatct gttcctcggg ctgatcatcg gggcgctcat ggaccggtgg agccgccagc
     1141 gcacactcgt gctgacgagc atcgggcgtg ccgtcgtgct ggccgtgatc ccggtgctgc
     1201 tgctgttcga cgcgctcacg ttctggtcgc tcgcagcggt gatgctcgcg ctgggtgtgc
     1261 tgacgctgtt cgcggattcc gcggcgcaac ccctgctgcc gagcctcgtc ccgcgtacgt
     1321 cgctggtgca ggcgaacgcc cgcctgggcc agagcgagac tgtcgcaggc accgccggcc
     1381 ccgcactggg cggggcgctg ctgagcctcg tcggtgcgcc gatcctgttc atcttcgaag
     1441 cggtgatcca tgcgtgcgcc gcagtgctgc aggcgcgcat ccccgtgcag gaggcttccc
     1501 cgccgccccg cgcaccgggc cggcacatcg gccacgagat cgccgagggc atgcggtaca
     1561 cctatcgcca ccgcacgctg cgtcccctgg cgatctcggt gcacatctgg ttcctcggca
     1621 acagcctggt tgccacgctg ttcgccgtgt acgtgatgcg ggagctgaag gtcgcaccgt
     1681 gggccttcgg cgtggccctc gccttcggcg ggatcggcgg gctcgtcgga gctctgctcg
     1741 cgcccgtgct cggcgcgaga ctcggggcgg ggcgcgcgat cctgctgggg cgggcgctgg
     1801 tggtgatccc gtgggcggcg ctcgctctga tcccggccga cgccctcggc acggtgctcg
     1861 tggcggtggt ggtgatctcg gccgtgcagt tcgtctacgg actgtcgatg ggcatcgagg
     1921 atgccaatga cagcggctat cggcaggccg cagcgccgga tgccattcaa gggcggatga
     1981 actcgaccat ccgcaccatg aaccggatca tcttcttctt cggagcgctg atcgccggtg
     2041 gcctggcgac gttcacgggc tactccatca ctttcgccat cgcggcatgc gtgttcgccc
     2101 tcgctgcggc cgtcgtcgtg ttctcaccgc tgcgtgacgc gcggcacgag acggcgcggg
     2161 tggagtgacc ggaaatcccc gcctcccggc ttcgcaggtc tcacgcgact tgcatgtacc
     2221 ctggttccgt gacgcggcag cagcgatttc agtacccccg gccgggatat ggccgcgggg
     2281 tcgtcgacgc gcgccgttag cgcatcaggt ctccgcaggt acggccgggg gaagcaccgg
     2341 accttcacca acctgcaatc gtctcggaga ccttctcatg tcgcatgccc gctatctgac
     2401 ttctgaccat cttcatcgtg ctctgtcact gcgcgacctc accgatcccg ctcaaggcga
     2461 ccacgcgatc cagatgctct tggactcggt ggtgaacacc ttgcgctcag ggtggcacag
     2521 caccgtccgc catgtgcggt cgacgccgat cgttccggtt cgagagaact acgaccgcct
     2581 ccggtaccac tcgggtgatg tcacccggga tcgccggtac acccgctacg tcagccggac
     2641 ggtgatgctg cgcagtcaca ccagcgcgga gctcccgacc gcactcgagg actacgcggg
     2701 acgaacgggg atcgacgagc tcatcgtcgc accgggcctc gtctaccggc gtgatgccgt
     2761 cgatcgcagt cacgtgggcg agcctcatca ggtggacctg tggcgcatcc gcagtgcagc
     2821 ggacaccgat gacagcgaca tgctcgtgat gatcgagcgt ctggtcgagg ctgtgctgcc
     2881 aggcgcccga tggagggtga cggatgccgt gcacccctac accgtcggcg gtcgccagat
     2941 cgatgtgctt cacaacggcg aatggctgga gctcgccgaa tgcggccgca tccatcccga
     3001 catcctcagc ggatcgggac tcgaccctgc gcgctggtcg ggcctggcac tcggtatggg
     3061 cctggaacgg gcgctgatgc tgcgcaaggg catccctgac atccgctacc tccgggccaa
     3121 ggagccgcgc atcgcctgtc agatgcttac tctggagccg tggcagcacg tctcacccct
     3181 acctgccacc cgacgagaca tctccgtggt gctggacgct gacgaggacg aggagacggt
     3241 gggcgaccgc atccgcatcg cgctcgggga cgatgcggac gtgatcgagt cggttgaggt
     3301 gctgagccgc accgctcacg aggaactcgc tgaggctgcc cgcgcgaggc tcgaaacgca
     3361 ggagggccag gtgaatctcc tgctgcgaat cgtcttgcgg ccgatcgatc gcacgctcac
     3421 ggcggaccag gccaacgcga tccggaacgc gatctaccgg gcggtgcacg aaggtccgag
     3481 gatggaattg atctgaagcc ggcgggcgcc cggtggccgg catccgggac gatgcgacgg
     3541 cacgtcccgg atgccggtgc gctattactc ggcgtacggc tgatccgggg cgtggtcgct
     3601 gtatccgata ctccagatgt agccgtccgg atcggcgaac gtgccgccgt atccgcccca
     3661 cggcaacgcg cctgcggggt tgaggaccgt cgcccctgcg gcctgcgcct cggcgatgac
     3721 ctcttcgaca cgcgcttcgg atcggacgac gtatgtcagc acgacgccgc tgaaaccgtt
     3781 gccgtccggg tccgtaccga cctgtgcggc gagcccgtcg cgtccgtaga agccgacggg
     3841 cgaggcgccg tctgattcga agaacactga gatgccgaag tcgctctgga ccttccaccc
     3901 gagtccctcg gtgtagaact tcttggcccg atccatgtcc cggacgccga gaagaatcga
     3961 gctgacgtgt gctttcacgc tgtactccct gttcgtgtga ggtgttcgtc accagactgc
     4021 gagaggtccg ccgcctcatc aacgggagat acagcgacag atcaatcaat cacgcttatc
     4081 acggagggaa taggctcgga ccaccggtgc gggcggagag gaatgcaatg gagcttcgag
     4141 acatcgagat cttcctgaca ctcgccgagg agttgcactt cggccgcacg gcgacgcggc
     4201 tgcacgtctc tcaggcgcga gtcagtcagg tcatcgccca gctcgagatg cagatcggcg
     4261 gaagcctgtt cgatcgctcg aaccgcagag atgtgcgcct cacgcctgtc ggcacccgac
     4321 tccgcgacga cctcatcccc gtccacgctg cgctgcaggg cagcctcgac cgcgcgaagc
     4381 tgtcagctcg cggcatcgac gcccgcctgc gcgtcgggct gatgccctac aacgtccccg
     4441 ttctgcacgg ctactgggac gccttcgcgc agcgccaccc gcgatatgag ctgcaggtgc
     4501 gccacgcttc gttcaccgac ccgttcggca gcctgcgcag aggcgagatc gacattctgg
     4561 tcacctggct ccccgtcgac gaagccgacc tgacggtcgg tcccaccttg ttcaccgact
     4621 cgcgtgtcct cgcggtctcc gtcgaccatg agctcgcagg gaggtcgtcg gttcctgtgg
     4681 agatgctggc ggacttcgcc catgtcaggg tgcccaacat gccagaggcg tgggagaacg
     4741 gctacctgcc cttccgcacg ccgagcggac ggacgataga gcgcgtggaa tccgtcacca
     4801 atgctgacga cctgcttcag ctcgtcagca ctggtgagat catccatccg ttcccggcgc
     4861 acgtagcgca ctactggtcc ttgcctcaga tccggtgggt gccgatcccg gacctgcccc
     4921 ggctcaccta tgcgctcgtg tggcgcacgg acgcggaaaa cgctccgatc cgcgctttcg
     4981 cccgaatgct cgcagagctc gggcccgtcg ccgagggaca tgagcgccga gccgattgat
     5041 aaccccggac gcaaggttct attcgcaatt cgtcgttgtt cgcattcagt caagtcgcga
     5101 ggctgaggac ggaatagccg atcccgcaca aggagagaga aatgacgacg acactcacgt
     5161 caacaccccg gatgcccagt cccgccgcga tcctcgatgg cgcatcgggg gcgatcttcg
     5221 cgctcatgaa agccgcccgg cagactgacc tggcgcactc gaccctcgaa ctggttcatc
     5281 tgcgagtgag ccagatcaac ggctgcagcc cctgcgtcga cgccggcgtg aagtcggcca
     5341 agcaggcagg cgagtccgac gaacgtctgc atgcggtggt cgcctggcgc gagacaccgt
     5401 acttcaccga ccaggagcgc gccgcgctgt cgttggccga gagcgccacg cggctggccg
     5461 accggtcgga tgccgtaccg gacgaggtgt ggaacgaggc cgcgagcttc ttcgacgagc
     5521 gtcagctggc cgcgctgatc ctctacatcg cgacgaccaa cctcttcaac cgtctcaacg
     5581 cgaccacccg ccagttcgcc ggcggctcct ggaactgaag gcacgatgat ggcaacagtg
     5641 cgaactcacc gctataccgt tgagacgggc agcctggtca cgatgctcga acgccgcacg
     5701 agcctgatcg acggcatccg gtccgccaat cccgccttcg ccgaggcgac cctgatcaag
     5761 ctggacgacg gttcctacct cgacatctgg cgctgggact ctgcggccga gatgcagcgc
     5821 gccttgcaga acgcgggaag catccccctg gtcggtgcga cgctgagcct gaccacggat
     5881 cacactgtca tcgacggaga ggtgctcgac cagcgctgat gcggcacacc tgcgccggtg
     5941 ccccgaatcg atcggggcac cggcgctttc cgtttgggaa tctacttgaa tagtcgtcaa
     6001 gtacgtgctt agcttgcaag caagcagatc tcaccgcaca gaccgttcct gacaaggagg
     6061 catcatgtcg aactctggag acagcaccgg cccctcaccc tcggcagcgg ccgaggcgct
     6121 cagttcagcg gatgcgacag ccggccgcat ccgctcgcgt cgccggtggt acatcgccgg
     6181 cacgctcgtg atggcggccg cactggcgac cttcagcatc gccctggcct cgtggccgga
     6241 tcgcctcgct gacgtgatca ttcctggcct cctcatcgtc ctcgcgatcc tcgctctcct
     6301 cgcgtggagc gggaggacca tcccggaggc ggcgacgaag gcgacgaacc gcgtggttct
     6361 catcagcgcc gtcatgatga tcgtgaccct gctgctgaac cgtctgcttc cggtcgggtt
     6421 ctccggatgg gtcgtcctca ccgggctgct gccggcgctg cccttcctct acctcgcttg
     6481 gcgggtggcc cgcgcatgac ccacccgcgt caccgcctgg acgacgtgat ccattctccc
     6541 gttcgcctct cggtcatggc ggcattgcgc cacgcggacg cggtccattt cgggttgctc
     6601 cgcgacaccg tggaggtcag cgactccctt ctgtccaagc acctcgcctc gctggaggcg
     6661 gccgggtacg tcgaggtgac gaagggatat gcgggacgcc gcccccgcac gtggtactcc
     6721 ctcacgaccg aagggcgaga agcgttcgat gcctacatca ctgcgctgcg agagatcgtg
     6781 gacgcgcccg agtcaccgtc gggtgccggc agctgatcag cggtcgctgt cccgagacaa
     6841 tgcaatcaat cgaagggcat gatgcgatgg tgatcgcgga tcttcctgcg ggcctggtag
     6901 ctcgccaccc tgtgccgaat gatcacccgc gagtgctgtc ggtgctcgac agatggtggg
     6961 cgaacctcaa gggcgacgac ggagcgcgcg agcgcgcact gctcctgccg aggctctact
     7021 tccagcattt cgcgaccacg agcttcgtgg tggaggacga gcacgggcaa ctcgctgcct
     7081 ttctcgtggg tttcctctcg cagaccgagt cggacgccgc gtacatccac ttcgtcggcg
     7141 tcgatcccct caggcagggg cagggcgtgg ggcgtgcgct ctaccgcgca ttcatcgatg
     7201 tcgccggcgc gaacggacga cacgtcgtgc gcagcgtgac cagccccgag aacgacgggt
     7261 cacgcgcgtt tcacagaagc ctcgggttct cagcgagcga ggtcatcccc gactacgacg
     7321 gcccggggct cgcgcgggtg gcattcagga cgacgacggc tgccgcccgc tcgcgaggag
     7381 tgcgggatct gcgcgtgctc gacggtgcgc tggtgctgga acggctcgat ggcatcgacg
     7441 ttcccgcggg tgattgggtc ggactcgtac gcgctccgga cgggctcacc gccattcgtc
     7501 cggccccggg ctcaggggcg aaggagcggt ggatcgccct gtacgaggca gacccggacc
     7561 acggcctgga cgaaccgggg ctgctcgtcg ccgtgctgac tccgctcgcg cggtcgggcg
     7621 tgcccgtctt cgtcgcgtcg acctaccgcg ccgacatggt cctggtcccc gaagaccgag
     7681 cgcagacggc tctcacggca ctgcggtcct ccggattcac gatctcgtga tgcgcagacc
     7741 gcgtcgtcaa ccggcagcgg atggccggag ctgcagccga tccaggtgcc ctgcgccgac
     7801 gggtgtgatc ctcagcgacc ggtcctcagc tcggcgcgtc acccagcccg ccgtcagcat
     7861 cgcctgaagc agcgccgcac ccaggcggcc cgctagatgt gggcggcggg cggtccagtc
     7921 gaggcaacct cgcacgagcg ggcgcgacga caccaccacc gtgagggaca gatcgagagc
     7981 cgatccgatc tcgtcgacgg cgacacgtgc cgaaccgagg tcttggtcga gcagtccccg
     8041 ctcgaccagg tggtccgcga ccgcgatgcc gaggtctcca gccaagtggt cgtagcagga
     8101 ccgggcgcgg cgcatcgcgg cgcgtcgatc gcttgccgcc caggacaaca cctccggctg
     8161 accggtcaga cgcagaacgg actccacggc ctctgccacc gcggggtcga cgatgcggac
     8221 caatctcgtg cggccctgct gctccacggc gatcagcccc gcgtcgagca gtctggtgac
     8281 gtgcgcggtc gtcgtcgacg gagcgatgcc gatccgggcg gcgagtgcgc cgaccgggag
     8341 cggtatgccg ccgagcagct cctgcaggat ctgcagcctg cggggctctg cgagtgcctg
     8401 cgccagctgc tcgagcgacg ggctggtcat ccgagcaccg tccgtgcacg ttcgtcgtcg
     8461 tcgacgacga acaccttgcg gttgtcgtga tcgctgtatt gcgcgtcgac tcggatgccg
     8521 gcggagtgca gcgccgacat catgcgcccc atctctccgg gcatgtctgc gcgcagaggc
     8581 accacgagcg cgggcgccga ggtcgcacgg atccctgcgg catccaaggc agcgaccgcg
     8641 acggcgccgt cggcgacgag gtagtgcgcc tgaccggccc acactccccc accctcgagg
     8701 cccacaccgg ccgcgccgag gactgcgccg atccggctga catctgcggc gacgtcgtcg
     8761 accgcgatga cgaggtcgtt cacgagagca cctccacgat cggcgtgagg gtcgcctccg
     8821 cggtgagcga gttggcgatg tagcatccgg cgtgcgcctc atggagaagc tcgaccacgc
     8881 gtgccgcttc cacatcccgc acagcaaccc gcggagccag ttcaatccga gtgatgcgcg
     8941 cgggccgctc tcccatcggc atgaacgccg tggcgtcgtc gtcatatcgc accacgtcga
     9001 caccggcctg cgccgcgacg gcgaggaacg agagcagcag gcacgagctc gctgcggcga
     9061 gcagcagctg ctcagggttg ggcagagacg cgtccccgcg gaacgcggcg tcggcgctga
     9121 gcgtgagaat ctcctcggag atctgcacgt cgtgctctcg cccgtatgcg cggtaacccg
     9181 cgcccgtgct gccctcccac gagagagcgg tgctgtactg gtgaacggtc atgcagccag
     9241 gctagaggcg ggacgtttcg gccggggacg aaatgtgcga agcgatggga tagccgatca
     9301 tgcgggcccc gcgtctaacc cagcttctgc ggactgattt cagcggactc cgcccaggta
     9361 cagagaaacc cccgatttct cgggggttct catggcggtg acggtgggat ttgaacccac
     9421 ggtagggggt taccctacac aacttttcga gagttgcacc ttcggccgct cggacacgtc
     9481 accgtcgtcc agcttacgat acggaccggc ctgcgcgcga atcgaggagc agaggtccgc
     9541 agactagcgc gtgacatatt accgagctag gctcgagtca tgcccgacgt catcgccgat
     9601 ctcaccctcg taggcgctca gtccatcgcc gagcgcgtca gcatgaccgc cgcggtcgcc
     9661 gagcttcagc gcatcctgcg cgccggcttc gatcccgagg gcgacctccc ccgcggcatc
     9721 ctcgacgtgc ccaacgggca gctgctgctc atgcccgctg ccgtcgacgg cgccgtcggc
     9781 cagaagttcg ccacggtcgc gcccggcaac cccgcgctcg gcctcgagcg catccaggcg
     9841 gtctacatcc tgctcgacgg gaccaccctc gcgccgaccg cgatcctcga cggcaccgcc
     9901 ctcaccagtc tgcgcacgcc cgcggtctcc atcgcgagcg tcgacctgct cgcccagccg
     9961 gacgccggcg ag
//
LOCUS       DATMSX010000364         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_128898,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000364 DATMSX010000000
VERSION     DATMSX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128898"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..968
                     /locus_tag="VN041_11300"
     CDS             69..968
                     /locus_tag="VN041_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU29659.1"
                     /translation="MASAPVPVGTARGAGSRRSASWAWLGLTPFAAYVLLFLALPTVL
                     ALASGLFTKTGAFTWSNLSALVDPAVLSTFASSTWVSLLTAIVGAVVGALVCYALLGM
                     NPEGAVRQSVDAAAGVLAQFGGVMLAFAFIATIGVQGIITTLLKDSFGVNIFEGGTWI
                     YEVPGLLLPYIYFQVPLMVITFMPALAALKPQWAEANATLGGTRASFWLHIGFPVLAP
                     SFLASLLLLFANAFSSYATAAALISQGSQVVPLQIRAALTSETVLGRENLAGALALGM
                     IVVVGVVMALYSLIQRRAARWQA"
     gene            965..1759
                     /locus_tag="VN041_11305"
     CDS             965..1759
                     /locus_tag="VN041_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU29660.1"
                     /translation="MNSLAPSRLTRWIIGILVGAFFAIPLISTFLYTLHDPKGGMSWI
                     NWHKLIDPAFASTLQPIWTGLGNSLVLAVVTVVIVLVLLAPTMILVHLRFPRLRPVFE
                     FLALLPISIPAIVLVVGLSPIYLQIARMLGTGTWTLAFAYGITVLPFAYRAIQASIDA
                     ADLRTLSEAARTLGASWPTVVVKVLAPNLRQGLLAASLISVAVVLGEFTIASLLNRQV
                     FQTAMVVVRNQDPYLPAIFTLLALLLVFVLLLIIGRAARGHRKARS"
     gene            1756..2841
                     /locus_tag="VN041_11310"
     CDS             1756..2841
                     /locus_tag="VN041_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29661.1"
                     /translation="MTAPLPRTQDNALLADAGEGTAVQLQGIVKSYSGNTVLHGVDLD
                     IAPGEFVSLLGPSGCGKTTLLRVLAGLEGSDSGAVLLGGKDVSRVPTNKRDIGMVFQS
                     YSLFPHLRVVENTAFGLRRRGVSKADAAKRALDALALVGLEEHADKFPHQLSGGQQQR
                     VALARALVTEPKVLLLDEPLSALDAKVRVQLRDEIRRIQLRLGITTVFVTHDQEEALA
                     VSDRIAVMSAGRIEQIGSPEELYGAPATASVAAFVGLSSVVGGTAEGASVEVWGQTLP
                     LQTPATGPVDVYLRPENVVFAEGADAAARGTVEESTFLGSMRRTVVRTEAGERVTVQH
                     SPGIRPAFGDAVGIALLGEPVAARPRD"
     gene            complement(2949..>4461)
                     /locus_tag="VN041_11315"
     CDS             complement(2949..>4461)
                     /locus_tag="VN041_11315"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HWU29662.1"
                     /translation="LLLAVAVNLVLAALTAVSIGSDDLRHPAELTAIDRAFPGFAVVA
                     ILVPLAVCTLLIVTRRRFAVHAEHGAGTRLAVVALLAAGVLGVADVVLLVSSDVDLGV
                     AALDALRRFLPAGFTHARIAERIGGVGNIARQVIGPLFWGVFTAAMLRLLRAPPAGAG
                     NVDDESYRALLRVHSGTLGFLGTWDGNGHWIAPDGRGGIAYRLVSGFAISVGDPVGAD
                     PDAVVHGFADFCDRQGWSPVLYSVHDPTLLTAQALGWRHMPVAEETVVPLERFSLDGK
                     AWAKVRQPYRRADRDGLHAEWTRWVDLTPGTSAQLDALSEQWIAEKALPEMGFTLGGI
                     EELKDPETALMIAIGPQGELQAVTSWLPVHDGGELVGWTLDFMRRADGSMPGVMEFLL
                     ASAALRMKADGLAFMSLSGAPLAQTADPDAAEGESQAMLGLLTWLGGVLEPAYGFRSL
                     LSFKAKFHPDRHALHMAYPDPAQLPAIGMALGRAYLPHVTTAEYLALARTLRA"
BASE COUNT          613 a         1657 c         1502 g          689 t
ORIGIN      
        1 atgcccacga cgcggagcgt cgggaccccg cgccgcgcgc ggtcccgacg gggacggggg
       61 cggatgccat ggcatccgcc cccgtccccg tcgggaccgc gcgcggcgcg gggtcccgac
      121 gctccgcgtc gtgggcatgg ctgggcctga ccccgttcgc cgcctacgtg ctgctgttcc
      181 tcgctctgcc gaccgtgctc gcgctcgcct cgggcctgtt cacgaagacg ggcgccttca
      241 cctggagcaa cctgtcggcg ctcgtggatc cggccgtgct cagcaccttc gcgagctcga
      301 cctgggtgtc gctgctcacc gcgatcgtgg gcgccgtcgt aggggcgctg gtctgctacg
      361 ccctgctggg catgaatccc gagggcgcgg tgcgccaatc ggtggatgcc gccgcgggtg
      421 ttctcgccca gttcggcggc gtcatgctcg ccttcgcgtt catcgcgacc atcggcgtgc
      481 aagggatcat caccacactg ctcaaggact ccttcggcgt gaacatcttc gaaggcggca
      541 cctggatcta cgaggtgccc ggcctgctgc tgccctacat ctacttccag gtcccgctca
      601 tggtgatcac gttcatgcct gccctcgccg cgctgaagcc gcagtgggcc gaggccaacg
      661 ccaccctggg cggcacccgc gcgagcttct ggctgcacat cggcttcccg gtgctcgccc
      721 cgtccttcct ggccagcctg ctgctgctgt tcgcgaacgc cttctcgtcg tacgccacgg
      781 cggccgcgct gatcagccag ggttcgcagg tcgtgccgct gcagatccgt gcggcgctca
      841 ccagtgagac cgtgctcggt cgcgagaacc tggccggcgc cctcgcgctc ggcatgatcg
      901 tcgtcgtcgg agtcgtgatg gccctgtact ccctcatcca gcgacgggct gcgaggtggc
      961 aggcatgaac tcgctcgccc cctcccgcct cacccgctgg atcatcggca tcctggtcgg
     1021 cgcgttcttc gccatcccgc tgatctcgac gttcctgtac accctgcacg accccaaagg
     1081 cgggatgtcg tggatcaact ggcacaagct gatcgacccc gcgttcgcga gcaccctgca
     1141 gccgatctgg acgggcctgg gcaactcgct cgtgctggcg gtcgtcacgg tcgtcatcgt
     1201 cctggtgctg ctggccccga ccatgatcct ggtgcacctg cgcttcccga ggctgcgccc
     1261 ggtgttcgag ttcctcgcgc tgctgcccat ctcgatcccc gcgatcgtgc tggtcgtcgg
     1321 cctgtcgccg atctacctgc agatcgcccg gatgctgggc accggcacct ggacgctggc
     1381 cttcgcgtac ggcatcacgg tgctgccctt cgcctaccgc gcgatccagg cgtccatcga
     1441 cgcggccgac ctgcgcacgc tctccgaagc ggctcgcacg ctcggcgcga gctggcccac
     1501 cgtcgtggtc aaggtgctcg cccccaacct gcgtcagggt ctgctcgcgg catccctgat
     1561 ctcggtggcc gtggtgctgg gcgagttcac catcgcctcg ctgctgaacc gtcaggtctt
     1621 ccagaccgcg atggtcgtcg tccgcaacca ggacccctac ctgcccgcga tcttcacgct
     1681 gctggcgctg ctgctcgtct tcgtcctgct gctcatcatc ggccgcgccg cacgcggcca
     1741 ccgaaaggcc cgctcatgac cgctccgctc ccccgcaccc aggacaacgc gctgctcgcc
     1801 gacgccggcg agggcaccgc cgtgcagctg cagggcatcg tcaagagcta cagcggcaac
     1861 accgtgttgc acggcgtcga cctcgacatc gcccccggcg agttcgtctc gctgctcggc
     1921 ccctccggct gcggcaagac cacgctgctg cgggtgctgg cgggcctcga gggctcggac
     1981 tcgggtgccg tgctgctggg cggcaaggac gtctcgcggg ttcccaccaa caagcgcgac
     2041 atcggcatgg tgttccagtc gtactccctg ttcccgcacc tgcgggtcgt cgagaacacc
     2101 gccttcggcc tgcgtcgtcg cggggtcagc aaggcggatg ccgcgaagcg cgccctcgac
     2161 gcgctggccc tggtggggct cgaggagcac gccgacaagt tcccgcacca gctctccggc
     2221 ggccagcagc agcgcgtggc gctcgcccgc gccctcgtca ccgagccgaa ggtgctgctg
     2281 ctggacgagc cgctctcggc gctggatgcc aaggtgcgcg tgcagctgcg cgacgagatc
     2341 cgccgcatcc agctgcgcct cggcatcacc acggtgttcg tcacgcacga ccaggaggag
     2401 gctctggccg tctccgaccg gatcgcggtg atgagcgccg gacggatcga gcagatcggc
     2461 tcgcccgagg agctctacgg tgctcccgcc acggcatccg tcgcagcctt cgtgggactc
     2521 tccagtgtgg tcggcggcac cgccgagggc gcgagcgtcg aggtctgggg tcagaccctc
     2581 ccgctgcaga ccccggccac gggcccggtc gacgtgtatc tccgtcccga gaacgtcgtg
     2641 ttcgccgagg gagcggatgc cgccgcacgc ggcaccgtgg aggaatcgac gttcctgggc
     2701 agcatgcgcc gcacggtggt gcgcaccgag gccggcgagc gggtgaccgt gcagcactcc
     2761 cccggcatcc gtcccgcgtt cggcgacgcc gtgggcatcg ccctgctggg cgagccggtc
     2821 gccgcgcgcc cgcgcgactg acggacaccg ccgagccacc atccagcgcc cgagccacca
     2881 cgcgaatcgc agtgaatcgc gtggtggctc ggcgttcgcg tggtggctcg gtgatcggat
     2941 gctggcgcct atgcgcgcag cgtgcgggcg agggccagat actccgccgt ggtgacgtgc
     3001 ggcaggtacg ccctcccgag cgccatcccg atcgcgggca gctgagcggg atccggatag
     3061 gccatgtgca gtgcatggcg atcggggtgg aacttggcct tgaagctgag cagcgaccgg
     3121 aagccgtacg cgggctccag cacgccgccc agccaggtca gcagcccgag catggcctgc
     3181 gactcaccct ctgcggcatc cgggtccgcg gtctgcgcga gcggcgcacc ggacaggctc
     3241 atgaacgcca gcccgtccgc cttcatccgc agggcggcgg aggccagcag gaactccatg
     3301 acgccgggca tggagccgtc cgcgcggcgc atgaagtcca gggtccagcc gacgagctcg
     3361 ccaccgtcgt gcaccggcag ccagctggtc acggcctgca gctccccttg cgggccgatc
     3421 gcgatcatga gcgcggtctc gggatccttc aactcctcga tgccgccgag ggtgaagccc
     3481 atctcgggca aggccttctc ggcgatccac tgctcgctca gggcgtccag ctgcgccgac
     3541 gtccccggcg tcagatcgac ccagcgggtc cattccgcat gcaggccgtc gcggtcagcg
     3601 cgccggtacg gctggcgcac cttcgcccag gccttgccat cgagtgagaa gcgctccagg
     3661 ggaacgacgg tctcctccgc gacgggcatg tgccgccagc ccagcgcctg cgcggtaaga
     3721 agggtcgggt cgtgcacgct gtacagcacg ggcgaccagc cctgcctgtc gcagaagtcc
     3781 gcgaagccgt ggacgacggc atccggatcc gccccgaccg ggtctcccac gctgatggcg
     3841 aagcccgaca ccagccggta cgcgatcccg ccgcgtccgt ccggggcgat ccagtgtccg
     3901 ttgccgtccc aggtgccgag gaaccccagg gtgccgctgt gcacgcgcag cagggcccgg
     3961 tagctctcat cgtcgacgtt ccccgcaccg gcgggcggcg cccgcagcag gcgcagcatc
     4021 gccgctgtga acacgcccca gaacaacggg ccgatcacct gccgtgcgat gttgccgacc
     4081 ccgccgatcc gctccgcgat gcgcgcgtgg gtgaagccgg caggcaggaa ccggcgcagg
     4141 gcgtcgaggg cggcgacccc cagatcgacg tcggatgaga cgagcagcac gacatcggcg
     4201 acgccgagca ctcccgccgc cagcagcgcg acgacggcga gcctcgtgcc cgccccgtgc
     4261 tccgcgtgca ccgcgaagcg ccggcgcgtc acgatcagca gggtgcagac ggccagtggg
     4321 acgaggatcg cgacgacggc gaagccgggg aacgcgcgat cgatcgcggt cagctcggcc
     4381 ggatgccgca gatcgtcgct gccgatgctc accgcggtca gcgccgcgag cacgaggttg
     4441 acggccacgg cgagcagcag c
//
LOCUS       DATMSX010000365         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_15004,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000365 DATMSX010000000
VERSION     DATMSX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15004"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..419)
                     /locus_tag="VN041_11320"
     CDS             complement(24..419)
                     /locus_tag="VN041_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU29663.1"
                     /translation="MPDIRVSAAVIVDRSGRVLVVRKQGTTRFMQPGGKPEPGETAAQ
                     TLIRELDEELSLHVEESDLEPLGTFVSAAANEPGHRVIADAFAMHAEPEDVVVQAEIA
                     ELRWLEPGDRTSVPLAPLSEEHLLAFAWR"
     gene            complement(412..>1560)
                     /locus_tag="VN041_11325"
     CDS             complement(412..>1560)
                     /locus_tag="VN041_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWU29664.1"
                     /translation="GLAGAANAGPGQIVLSTAEMDRIIEVRPDDLLAVVEPGILNATL
                     NAQLAEHDVWWAPDPASREISTVGGNIATGAGGLLCAKYGVVRDAVLGVDLVLADGRL
                     LRLGHRTVKGVTGLDLTSLVIGSEGRLGVIVGATLKLRRLVEGGTCTITATFDSVRDA
                     AAASAAVTASGAQPAIMELMDARMLAAVHDLLGLPVSDAGTSQLTIQTDGPGAREEAA
                     RIVAVLRAAGGAVVETDDPVEGERLLAIRRSTHPALAALGTTLIEDVAVPRSAMPAMF
                     DEIARIEQEYGVVIPTVAHAGDGNLHPNFIFDGDEVPDHIWEAAGELFRAAIRLGGTL
                     TGEHGIGMLKSRWLAEELGEDQWRIQRDIVRVFDPQGILNPGKVFSDA"
BASE COUNT          220 a          571 c          545 g          224 t
ORIGIN      
        1 cgttctcggg ctgcctgccg gcgtcagcgc caggcgaacg ccagcaggtg ctcctcgctg
       61 agcggtgcca gtggcacgct cgtgcggtcg ccgggctcca gccatcgcag ctcggcgatc
      121 tcggcctgca cgacgacgtc ctccggctct gcgtgcatgg cgaaggcatc cgcgatcacc
      181 cggtgacccg gctcgttggc cgccgccgag acgaaggttc ccagcggctc gagatcggac
      241 tcctcgacgt gcagcgagag ctcctcgtcg agttcgcgga tcagcgtctg cgccgcggtc
      301 tcgccgggct ccggcttgcc gcccggctgc atgaagcgcg tggtgccctg cttgcggacc
      361 acgagcacac ggcccgagcg gtcgacgatc acagcggcgc tgacgcggat gtcaggcatc
      421 ggagaacacc ttcccgggat tcaggatccc ctgcggatcg aagacgcgca cgatgtcgcg
      481 ctggatccgc cactggtcct cgcccagctc ctcggccagc cagcggctct tgagcatccc
      541 gatgccgtgc tcgccggtca gcgtgccgcc gaggcggatc gcggcgcgga acagttcgcc
      601 ggcggcctcc cagatgtgat cggggacctc gtcaccgtcg aagatgaagt tcggatgcag
      661 gttgccgtcg ccggcgtgcg ccaccgtcgg tatgacgacg ccgtactcct gctcgatgcg
      721 cgcgatctca tcgaacatcg ccggcatcgc gctgcgcggc acggccacgt cctcgatcag
      781 cgtggtgccc agtgcggcca gtgccgggtg cgtggaacgg cggatggcga gcaggcgctc
      841 accctccacc gggtcatcgg tctcgaccac tgcaccgccg gccgcccgga gcaccgccac
      901 gatgcgcgcg gcctcctcgc gggcgcccgg accgtcggtc tgaatggtga gctgcgacgt
      961 cccggcgtcg gagacgggca gacccagaag atcgtgcacg gccgcgagca tgcgggcgtc
     1021 catgagctcc atgatggccg gctgcgcacc ggaggcggtg accgcggccg aggccgcagc
     1081 ggcgtcgcgg actgaatcga aggtcgcggt gatcgtgcag gtgccgccct ccacgagccg
     1141 gcgcagcttg agggtcgcac cgacgatcac gcccagccgc ccctccgagc cgatcaccag
     1201 cgacgtgagg tccagcccgg tgaccccctt caccgtgcga tggccgaggc gcagcagccg
     1261 gccgtcggcc agcacgaggt ccacacccag caccgcgtcg cggacgacac cgtacttcgc
     1321 gcagagcagc ccgcctgcgc ccgtggcgat gttgccgccc acggtcgaga tctcgcggct
     1381 ggcgggatcc ggtgcccacc acacgtcgtg ctccgcgagc tgcgcgttca gcgtcgcgtt
     1441 gaggatgccc ggctcgacga ccgcgagcag gtcgtcgggg cgcacctcga tgatgcggtc
     1501 catctccgcg gtggacagca cgatctggcc gggcccggcg ttcgcggcgc cggcgaggcc
//
LOCUS       DATMSX010000366         5072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166489,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000366 DATMSX010000000
VERSION     DATMSX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166489"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VN041_11330"
     CDS             complement(<1..2360)
                     /locus_tag="VN041_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HWU29665.1"
                     /translation="MLLLLAVFLIGSLLLPVLVRWLGAQAFVIAALVPAAAFVHALTL
                     TSQVLDPKTIPFQDYEWIPQLGLHLSMRMDVLGWVLTLIVTGVGALVLLYCRWYFHDD
                     GVEVGQFAGVLLGFAGAMYGLVLTDDLIMLVMFWEVTSILSYLLIGHYRRRAASRRAA
                     LQALLVTTLGGLVLFVGAVLLVVDAGTASIRELLELAPTGPIVDAAVVMLLIGAVSKS
                     AIFPFHFWLPGAMAAPTPVSAYLHAAAMVKAGIYLIARFAPIFALSGPWRPIVISLGV
                     ITMLLGGIQALRETDLKRILAFGTVSQLGFFAVVIGFGTPGAALAGLALVIGHALFKS
                     ALFLIVGVIDRQLSTRDITELSGVGRQAPVMATAAFISVASMMGVAPTIGYVAKESTL
                     TALLEDAQHGSVWGWVALIGVTLGAVLTVAYGCRFLWGAFWRKKHASGAYMPETAWPD
                     PPVGFLASPIILAGLSIAAGIGAPAVDVALQGYARSVDPAGDGASPGHLALWHGFEPA
                     LFLSLGAIALGIGTFVLSIRSGWHRWPRLLRFTAADVYYLTMRGVDRLSVLTTSLTQR
                     GSLPLYVGTIFVVLLAAEATALIGAAPERLALSAWHTPVQIVVAPIMAVAGVIAVRAQ
                     KRYTGVVLVSVTGLGMVVLFATSGAPDLALTQILVETVTMVTFALVLRRLPSRMGEHN
                     ASVGRVPRAVLAIGVGITMALVAIVATQARIFDPISVDFPQLAYDLGHGKNVVNVALV
                     DLRGWDTMGELSVLVLAATGVASLVFVTHRADLLSTTRDLPQTARRR"
     gene            2522..4156
                     /locus_tag="VN041_11335"
     CDS             2522..4156
                     /locus_tag="VN041_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29666.1"
                     /translation="MRGAWREASGWAAAVAMSTVIVGQVASTARSELLFRDGDSLIVA
                     LVARSILDHDRLDWMFSGVLFLPETAVYTVLRMLLPALSVMGVLAVNAVVNLLALYGA
                     IRLAAGRRRPGRAPVAWSLIALGAFGMLALTETSSSRDALEPASLLLTTTYYSATVIG
                     AVLAVGLVRRWFDARGSGSSGSSGSSSPLLSVFAPAQSRHSSGARTTSRGEDARRAPG
                     RAALIALSGVVVVSTVTNPLFLAWATAPLLVLLLIALHPRLCGVPALRPLLVVAGGSV
                     LGMLIRIPLSAWIADPGTGYAHPTELLRSAEYYGALAGDRLITPAGIGATALTFALLL
                     ICLLRTFRPGSRDDDSRAGGMLLAAYGWFTPLATVVGAILLGTHAARYLEPIAFAPVL
                     GLVAHPRSIRLPSAVKVTAAIGAVLLAAGAAASVPRIHAAATAPDPDAACVVDWVNAS
                     GRTGGGQFWTVRLPKLLLKDPAQLVQVDHTLRGYNWLANRTDFAAGELTFLVEDAQTV
                     RWQIADNPQPDAVIGCGRYRILDFASQPLKIGPQRS"
     gene            complement(4172..>5072)
                     /locus_tag="VN041_11340"
     CDS             complement(4172..>5072)
                     /locus_tag="VN041_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU29667.1"
                     /translation="ASLRTGRSGIVAVVLGGELRHAFLDPVTTAMMDGLSDALAAISA
                     GILLLRDDPQGDEAPPITEAPVDAVVLIGCSGRTRSALETVRQRGLPVVVIEGDAGEG
                     IPQVTLDNREASAEMADYVCQLGHTDVATVTLALDAERERAPITPARVAAATVDVTLD
                     RLAGFREIYPDAPGVTAAASLVDEGMIAARTLLADDTGSLRADRPTAIVAQSDLLAVG
                     VIRAAEELGLRVPDDLTVVGFDGINADGLGSLRLTTIAQPAVAKGRAAGEQVARMLAG
                     EPGESVHFTCSFREGETAAPPAR"
BASE COUNT          781 a         1810 c         1768 g          713 t
ORIGIN      
        1 cggcgccgcg cggtctgcgg caggtcgcgg gtcgtcgaga gcaggtcggc ccggtgcgtg
       61 acgaagacca gggaggccac gccggtggcg gcgagcacga gcacgctgag ctcgcccatc
      121 gtgtcccaac cgcgcaggtc gacgagcgcc acgttcacga cgttcttgcc gtgcccgagg
      181 tcgtaggcca gctgcgggaa gtcgaccgag atcgggtcga agatgcgcgc ctgcgtggcg
      241 acgatcgcga ccagcgccat ggtgatgccg acgccgatcg ccaggacggc gcgcgggacg
      301 cgtccgacgg atgcgttgtg ctcccccatc cgcgagggca gccgcctcag caccagggcg
      361 aaggtgacca tggtgacggt ctcgacgagg atctgcgtga gcgccaggtc gggggcgcca
      421 ctcgtcgcga acagcacgac catgcccagg ccggtcaccg agacgagcac gacgccggtg
      481 tagcgcttct gcgcgcggac ggcgatgacc ccggcgacgg ccatgatcgg tgcgacgacg
      541 atctgcaccg gggtgtgcca cgccgagagg gcgagtcgct cgggtgccgc gccgatgagc
      601 gcggtggcct ccgccgcgag cagcaccacg aagatcgtgc cgacgtacag cggcagcgaa
      661 ccgcgctgcg tcaggctcgt ggtcagcacg gacaggcggt cgacgccgcg catggtcagg
      721 tagtagacgt cggccgcggt gaatcgcagc aggcgcggcc agcggtgcca gccggagcgg
      781 atgctgagca cgaacgtccc gatgcccagg gcgatcgcgc ccagcgacag gaacagcgcc
      841 ggctcgaatc cgtgccagag cgcgaggtgt ccgggagatg cgccgtcacc cgcgggatcc
      901 acgcttcgcg cgtacccctg cagcgcgacg tcgacggccg gcgctccgat gcccgcggcg
      961 atgctgagcc cggcgaggat gatcggcgat gccaggaagc cgaccggcgg atccggccat
     1021 gccgtctcag gcatatacgc gccggacgcg tgcttcttcc gccagaaggc gccccacagg
     1081 aatcggcatc cgtacgccac ggtcagcacc gcgccgaggg tgacgccgat cagggcgacc
     1141 catccccata cggatccgtg ctgcgcgtcc tcgagcagcg cggtgagcgt ggactccttc
     1201 gcgacgtacc cgatggtcgg ggcgacgccc atcatcgagg cgaccgagat gaacgccgcg
     1261 gtcgccatca ccggcgcctg acgtccgaca cccgagagct cggtgatgtc acgcgtggag
     1321 agctggcggt cgatgacacc gacgatcagg aacagcgccg acttgaacag cgcatgcccg
     1381 atgaccagcg cgagtccggc gagggcggca cccggcgtgc cgaacccgat cacgaccgcg
     1441 aagaagccca gctggctgac cgtgccgaat gcgaggatgc gcttgaggtc ggtctcgcgc
     1501 agggcctgga tgccgcccag cagcatcgtg atgacgccga gcgagatgac gatcgggcgc
     1561 cacggcccgg agagcgcgaa gatcggcgcg aaccgggcga tcaggtagat gccggccttc
     1621 accatggcgg ctgcgtgcag gtaggcactc accggcgtgg gcgccgccat cgcaccgggc
     1681 agccagaagt ggaacgggaa gatcgcggac ttcgacacgg cgccgatcag cagcatcacg
     1741 accgcggcat ccacgatcgg gccggtgggc gcgagctcga gcagctcgcg gatgctggcc
     1801 gttccggcat ccaccaccag caggacggcg ccgacgaaca gcaccagccc acccagcgtg
     1861 gtgaccagca gcgcctgcag cgcggcacga cggctggctg cacgacgccg atagtggccg
     1921 atgagaaggt acgacaggat gctggtgact tcccagaaca tcacgagcat gatcagatcg
     1981 tcggtcagca ccagcccgta catggcaccc gcgaaaccga gcagcacgcc cgcgaactgg
     2041 ccgacctcga ccccgtcgtc gtggaagtac caccggcagt agagcagcac gagcgcgccg
     2101 acgccggtga cgatgagcgt caggacccag ccgagcacgt ccatccgcat ggagaggtgc
     2161 agacccagct gcgggatcca ctcgtaatcc tggaacggga tcgtcttcgg gtccagcacc
     2221 tgcgacgtga gcgtgagcgc gtgcacgaac gcggcggcag gaaccagcgc cgcgatcacg
     2281 aatgcttgtg ctccgagcca gcgcaccaga accgggagaa gcagcgaccc gatcaggaac
     2341 acggcgagga gcaacagcat aagcggctcc tcaagctcgt cggggccttc cggcacgggc
     2401 gggcgaatct ccgtccattc tacgggggcg atggggccac cggtcgccgg agtccgccgg
     2461 gcaccgcacg cgtggctgcg atctcagccg tccgacaggc ccgccgtgcg agagtgaacc
     2521 catgcgtggg gcatggcgcg aggcgtcggg gtgggctgct gcggtcgcga tgtcgacggt
     2581 gatcgtcgga caggtcgcgt ccaccgcgcg ctccgagctg ctgttccgcg acggcgattc
     2641 cctcatcgtc gccctcgtgg cgcggtcgat cctggatcac gaccggctcg actggatgtt
     2701 ctcgggcgtg ctgttcctgc cggagaccgc ggtgtacacg gtgctgcgga tgctgctgcc
     2761 cgcactttcg gtcatgggcg tgctggcagt gaacgccgtg gtgaacctgc tggccctgta
     2821 cggcgcgatc cgcctcgccg cgggccggcg acgccccggc cgcgcaccgg tggcctggtc
     2881 gctgatcgcc ctgggcgcgt tcgggatgct ggcgctcacc gagacctcct cctcgcggga
     2941 tgccctggag ccggcgtccc tgctgctgac caccacctac tactcggcca ccgtgatcgg
     3001 cgccgtgctt gcggtcggtc tggtgcggcg ctggttcgac gcgcgtgggt cgggttcgtc
     3061 aggttcgtcg ggttcgtcct cgcccctgct cagcgtcttc gcccctgctc agtcacgtca
     3121 ttcatcaggg gcgaggacga ccagcagggg cgaggatgca cgtcgggctc cgggacgcgc
     3181 ggcgctcatc gcgctgagcg gggtcgtggt cgtctccacc gtcaccaacc ctctgttcct
     3241 cgcctgggcg acggctcccc tgctcgtgct gctgctgatc gccctgcatc cgcggctgtg
     3301 cggcgtgccc gcactgcgcc ccctgctcgt cgtcgcgggc ggctcggtcc tggggatgct
     3361 catacgcatt ccgctgtcgg catggatcgc ggaccccggc acggggtacg cgcatccgac
     3421 cgagctgctg aggtcggccg agtactacgg tgccctggcg ggcgaccgtc tgatcacgcc
     3481 ggccggcatc ggcgccaccg cactgacctt tgcgctgctc ctgatctgcc tgctgcgcac
     3541 gttccgaccc ggatcccgcg acgatgactc aagagccgga gggatgctgc tggccgccta
     3601 cggctggttc accccgctcg cgacggtcgt cggcgcgatc ctgctgggca cacacgcggc
     3661 tcgctatctc gagccgatcg ccttcgcccc ggtgctcgga ctcgtcgccc atccgcggtc
     3721 gatccggctc ccctctgccg tgaaggtcac agccgcaatc ggcgcggtgc tgctggctgc
     3781 gggagctgcg gcatccgtcc cgcgcatcca tgcggccgcg accgcacccg atccggatgc
     3841 cgcgtgcgtc gtcgactggg tgaacgcctc gggccgcacc ggcggcggtc agttctggac
     3901 ggtgcgcctg cccaagctgc tgctgaagga tcctgcacag ctggtgcagg tcgatcacac
     3961 cctgcgcggc tacaactggc tcgcgaaccg caccgacttc gctgccggcg agctgacctt
     4021 cctcgtcgag gacgcccaga ccgtgcgctg gcagatcgcc gacaacccgc agccggacgc
     4081 cgtgatcgga tgcggtcgct accgcatcct cgacttcgcg agccagccgc tgaagatcgg
     4141 cccccagcgc tcctgagagc acgcgaaggg atcagcgcgc cggcggcgcc gcggtctccc
     4201 cctcgcggaa cgagcaggtg aagtgcacgg actcccccgg ttcgccggcc agcatccgcg
     4261 ccacctgctc ccccgccgcc cggcccttgg ccacggcggg ctgcgcgatg gtggtgagtc
     4321 gcagcgaacc gagcccgtcg gcgttgatgc cgtcgaaccc gaccacggtg aggtcgtccg
     4381 gcacccgcag gcccagctcc tccgcggcgc ggatcacgcc caccgccagc aggtcgctct
     4441 gcgcgacgat cgcggtggga cgatcggcgc gcagggagcc ggtgtcatcc gccagcagcg
     4501 tccgggccgc gatcattccc tcatcgacca ggctcgccgc ggcggtcaca cccggcgcat
     4561 ccgggtagat ctcgcggaac cccgccagtc ggtccagggt gacgtcgacc gtcgccgccg
     4621 ccacgcgagc gggtgtgatc ggagcccgct ctcgctccgc gtcgagggcg agcgtcacgg
     4681 tcgccacgtc cgtatgcccg agctggcata catagtccgc catctccgca ctggcctcgc
     4741 ggttgtccag cgtgacctgg ggaatcccct cccccgcatc gccttcgatc acgaccacgg
     4801 gaagcccccg ctggcgcacg gtctccagcg cggaacgggt gcggcccgag catccgatca
     4861 gcaccacggc gtcgaccggc gcctcggtga tcggcggagc ctcatcaccc tggggatcgt
     4921 cgcgcagcag caggatgccg gcgctgatcg cggcgagcgc gtcggagagg ccgtccatca
     4981 tcgcggtggt caccgggtcg aggaaggcgt gccgcagctc tcctccgagc accaccgcca
     5041 cgatcccgct gcgcccggtg cgcagcgacg cg
//
LOCUS       DATMSX010000367         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_119591,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000367 DATMSX010000000
VERSION     DATMSX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119591"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1374
                     /locus_tag="VN041_11345"
     CDS             133..1374
                     /locus_tag="VN041_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HWU29668.1"
                     /translation="MTEPTALTVAIIPARGGSKGVPGKNLRRVGGVPLVERAVRAASA
                     ASGVDLVVVSTDDAAISAVAEGAGARVIARPAELAGDTASSESALLHALDVLESEGAR
                     VGTIVFVQATSPFIPSEGIAEAVDQIVSGRFDSVFSAYETYGFLWRRNAEGRAAAINH
                     DAARRPRRQDREPHYLETGAFYAFDAVGFRRFQHRFFGQVGIVEVPESSAVEIDDEQQ
                     LALAEALAAASAAPQNFGEVRHRGADATRASEILHISEVLPEPITASVLVTDFDGVHT
                     DDTATVDSEGRESVRVSREDGMGVSRLRRAGIPMLILSTEQNPVVARRAEKLQVPVLH
                     GIDDKASALAAWAAEHGIRLADIAYVGNDVNDLGALGIVGWPIAVANAHPQVKAAARV
                     VLSRRGGEGAVREVIERMLPH"
     gene            1619..2491
                     /locus_tag="VN041_11350"
     CDS             1619..2491
                     /locus_tag="VN041_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HWU29669.1"
                     /translation="MTVSIADRVVGGGRPTYVIAEIGLNHNGDVEIAKQLIDVARAAG
                     ADAVKFQKRTPEIATPEHMRDVQRETPWGTMSYLDYRRRVEFGRDEYVEIGDYATMAG
                     LHWFASPWDVPSVEFLEDLNVVAHKVASASLTDTELLQALRDTGKPVILSTGMSTIEQ
                     IDTALETLGTDRTILMHATSTYPMEPGEANLKMISTLRDRYAGIPVGYSGHERGLQIS
                     LAAVALGAVAVERHITLDRAMWGSDHAASLEPGGLERLVRDIRVIEEALGDGVKQVYP
                     GELAPMAKLRRVGA"
     gene            2488..>3011
                     /locus_tag="VN041_11355"
     CDS             2488..>3011
                     /locus_tag="VN041_11355"
                     /inference="COORDINATES: protein motif:HMM:NF042780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HWU29670.1"
                     /translation="MTYPGSRPPRLRVVAIGDADSFVKWAAHLVDRVDDISQHLLLVQ
                     TPLVVSDAQKDAALAGTRFAGDPRSVTRIAFADVVDWLASDVPDVVVLAGRAPFVRLM
                     SRQIDRLSRRPVVVSGLPGMSIPALRGALEYRRHCDLMVVHSRREVAAYARLGRAIGV
                     TVPVALATLPFAHRT"
BASE COUNT          446 a         1087 c         1044 g          434 t
ORIGIN      
        1 cacgccgtcg cgtccgccgc acgtccgcgc atcccgagcc gcgcgtgcgc tgtcgcgcgc
       61 gttcgtcgca tccgtgccgt cgatcgatgg gccgcgtgcg agcagcgtgc gcggatgcag
      121 gaagaggcag tcatgacgga accgacggcg ctcacggtcg cgatcatccc cgcgaggggc
      181 ggttcgaagg gcgtaccggg caagaacctg cgtcgcgtcg gtggcgttcc gctcgtcgaa
      241 cgggcggtgc gtgcggcatc cgcggcatcg ggtgtggacc tcgtcgtggt ctcgaccgac
      301 gacgccgcca tctcggcggt cgccgaaggc gccggtgcac gggtgatcgc ccgcccggcg
      361 gagctcgccg gcgacaccgc gtcgtcggag agcgcgctgt tgcacgcgct cgacgtgctg
      421 gagtccgagg gggcgcgggt cggcaccatc gtgttcgtgc aggcgacctc gccgttcatc
      481 cccagcgagg ggatcgccga ggccgtcgac cagatcgtct cgggccggtt cgacagcgtc
      541 ttctcggcat acgagaccta cggcttcctc tggcggcgca acgccgaggg cagggccgcc
      601 gcgatcaacc acgacgccgc ccgcaggccg cgccggcagg accgagaacc gcattacctc
      661 gagaccgggg cgttctacgc cttcgacgcc gtcggattcc gccgcttcca gcatcgtttc
      721 ttcgggcagg tcggcatcgt cgaggtgccc gagagctcgg ccgtcgagat cgacgacgag
      781 cagcagctcg ccctcgcgga ggcgctcgcc gcagcatccg ccgcgcctca gaacttcgga
      841 gaagtgcggc atcgcggagc ggatgcgacc agagcatccg agatcctgca catctccgaa
      901 gtcctgcccg agccgatcac agcatccgtc ctggtcaccg acttcgacgg cgtgcacacc
      961 gacgacaccg ccacggtcga cagcgagggg cgcgagtcgg tgcgggtgag cagagaggac
     1021 ggcatgggag tgtcgcggct gcgccgcgcc ggcattccga tgctgatcct ctccaccgag
     1081 cagaaccccg tcgtcgcccg ccgagccgag aagctgcagg tgcccgtgct gcacggcata
     1141 gacgacaagg cctccgcgct cgccgcctgg gcggcggagc acggcatccg cctggccgac
     1201 atcgcgtacg tcggcaacga cgtgaacgac ctcggcgcgc tgggcatcgt cggctggccg
     1261 atcgccgtcg cgaacgcgca tccgcaggtg aaggccgctg cccgcgtcgt gctctcccgc
     1321 cgcggcgggg agggcgccgt ccgcgaggtg atcgagcgga tgctgccgca ctgaccctgt
     1381 gcctcggttc cacttcgggg ggccgacacg ccagatctcg gacgtctccc gcgaatcggt
     1441 ccgaaaagtg gcgtgtcggc ccccgatctg cgtcgtgcac ggcgcggccg gccacggttc
     1501 agcgggcgag ttcgtcgcat gggcttccgc gaacggtcac ccgagcgacg acggcggttc
     1561 atctggcgcc cgtagaccag gaagacggac cggagaacca atcgaacgga gcgtcctcat
     1621 gaccgtcagc atcgcagacc gggtggtcgg tgggggccgt cccacctacg tcatcgccga
     1681 gatcggcctg aaccacaacg gcgacgtcga gatcgccaag cagctcatcg acgtggcgcg
     1741 cgccgccggc gcggatgccg tgaagttcca gaagcgcacg cccgagatcg ccacgcccga
     1801 gcacatgcgc gacgtgcagc gcgagacgcc ctggggcacc atgagctacc tcgactaccg
     1861 ccgccgcgtg gagttcggcc gggacgagta cgtcgagatc ggcgactacg ccacgatggc
     1921 gggcctgcac tggttcgcct caccgtggga cgtgccgagc gtcgagttcc tcgaggatct
     1981 caatgtcgtg gctcacaagg tcgcatctgc gagcctgacc gacaccgagc tgctgcaggc
     2041 cctgcgcgac accggcaagc ccgtgatcct ctccaccggc atgtccacga tcgagcagat
     2101 cgacaccgcg ctcgagaccc tcggcaccga ccgcacgatc ctcatgcacg ccacctccac
     2161 ctatccgatg gagccgggcg aggcgaacct caagatgatc tccacgctgc gcgaccgcta
     2221 cgcgggcatc ccggtcggct actccggcca cgagcgcggc ctgcagatct ccctcgccgc
     2281 ggtcgccctc ggcgccgtcg cggtggagcg tcacatcacg ctcgaccgcg ccatgtgggg
     2341 ttccgaccac gccgcttcgc tcgagccggg cgggctggag cgcctggtgc gcgacatccg
     2401 cgtgatcgag gaggccctcg gtgacggcgt caagcaggtc taccccggcg agctcgcgcc
     2461 gatggccaag ctccgccgcg tgggcgcatg acctatcccg gcagccgccc gccccggctg
     2521 cgcgtcgtcg cgatcggcga cgccgactcg ttcgtcaagt gggccgcgca cctcgtggac
     2581 cgcgtcgacg acatcagcca gcacctgctg ctggtgcaga cgccgctggt cgtcagcgat
     2641 gcgcagaagg atgctgcgct cgccggcacc cggttcgccg gcgatccgcg cagcgtgacg
     2701 cgcatcgcct tcgccgacgt cgtcgactgg ctcgccagcg acgtgccgga tgtcgtggtg
     2761 ctggcgggcc gggcgccgtt cgtgcgtctg atgagccggc agatcgaccg actcagccgt
     2821 cgcccggtcg tggtcagcgg acttcccggg atgtcgatcc cggcgctgcg cggagccctg
     2881 gagtaccgcc ggcactgcga tctgatggtg gtgcactccc gccgggaggt cgccgcgtac
     2941 gcgcgcctgg gtcgtgcgat cggagtgacg gtgcccgtcg ccctggccac gctgccgttc
     3001 gcgcaccgca c
//
LOCUS       DATMSX010000368         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166503,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000368 DATMSX010000000
VERSION     DATMSX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166503"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1531)
                     /locus_tag="VN041_11360"
     CDS             complement(32..1531)
                     /locus_tag="VN041_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWU29671.1"
                     /translation="MTQARISIDREAVVAPIDRRIFGSFVEHLGRCVYDGIYEPGHPT
                     ANEDGFRLDVVDLVRELGSTTIRYPGGNFVSGFRWEDSVGPRENRPVRRDLAWHSLET
                     NQVGVDEFARWLKLTGSELMMAVNLGTRGIEAALDLLEYCNHPSGTALSDQRIANGTT
                     EPHDIRMWCLGNEMDGPWQTGYMTADDYGKLAARTAQAMKAADRTLELVVCGSSGSSM
                     PTFGDWERTVLEHAYEHVDFISCHAYYQERGGDLDSYLASSLDMQYFIETVVASADHV
                     GYKLKSKKKIKLSFDEWNIWYLDEHQASDEVNDEWRYAPRQLEDVYSVADAVVLGNLL
                     ITLLKNHDRVASASLAQLVNVIAPIMTEPGGDAWRQTTFFPFSVTSRLAKGEIIRPRI
                     EADTYATKVYGDAPLVDSVVTTDGEGSAVFLVNRSRSSAITVTIDADELGVTSVDEAV
                     TLWDDDVYAKNTLADQNRVGLKPLEGAVLADGVLTVTLPPVSWSALALS"
     gene            complement(1558..>1830)
                     /locus_tag="VN041_11365"
     CDS             complement(1558..>1830)
                     /locus_tag="VN041_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29672.1"
                     /translation="VLVVLTGVVAVTLLARYDDRPAAVLRYALGIALTAPLTATGVVL
                     ALVSCGIIAAVLPVFVPLAGASLPLGIAARLIDRRLRTLDPGHPDH"
BASE COUNT          282 a          592 c          666 g          290 t
ORIGIN      
        1 gccgcgccgg ccaggcgcag gatcgaacgc ctcacgacag cgcgagcgcc gaccatgaga
       61 ccgggggcag cgtcacggtg agcacgccgt cggcgagcac ggcgccctcc agcggcttga
      121 gcccgacgcg gttctggtcg gcgagcgtgt tcttggcgta cacgtcgtcg tcccacagcg
      181 tgaccgcctc gtcgaccgag gtgacgccga gctcgtccgc gtcgatcgtg acggtgatgg
      241 ccgagctccg gctgcggttc accaggaaca ccgccgagcc ctcgccgtcg gtcgtgacca
      301 ccgagtcgac cagtggcgcg tcgccgtaga ccttcgtcgc gtaggtgtcc gcctcgatgc
      361 gcggcctgat gatctcgccc ttggccaggc gcgaggtcac cgagaacggg aagaacgtgg
      421 tctggcgcca ggcgtcgcca ccgggctcgg tcatgatcgg ggcgatcacg ttgaccagct
      481 gcgccagcga cgccgaggcg acccggtcgt ggttcttcag cagcgtgatg agcaggttgc
      541 cgagcaccac ggcatccgcc accgagtaga cgtcctccag ctgacgcgga gcgtaccgcc
      601 actcgtcgtt gacctcgtcg gatgcctggt gctcgtcgag gtaccagatg ttccactcgt
      661 cgaaggagag cttgatcttc ttcttcgact tcagcttgta gccgacgtgg tcggcgctgg
      721 ccaccaccgt ctcgatgaag tactgcatgt ccagcgacga cgccaggtac gagtccaggt
      781 caccgccgcg ctcctggtag taggcatggc aggagatgaa gtccacgtgc tcgtacgcgt
      841 gctcgagaac ggtgcgctcc cagtcgccga aggtcggcat cgacgacccg gacgatccgc
      901 acaccacgag ctcgagggtg cgatcggcgg ccttcatggc ctgcgcggtg cgcgcggcga
      961 gcttgccgta gtcatcggcg gtcatgtatc cggtctgcca gggtccgtcc atctcgttgc
     1021 ccaggcacca catccggatg tcgtgaggct cggtcgtgcc gttggcgatc cgctgatcgc
     1081 tcaatgccgt gccggacggg tggttgcagt actcgagcag gtcgagcgcc gcctcgatgc
     1141 cgcgggtgcc gaggttcacg gccatcatca gctcggagcc ggtgagcttc agccagcggg
     1201 cgaactcgtc gacgccgacc tggttggtct ccagcgagtg ccaggccagg tcgcggcgca
     1261 ccgggcggtt ctcacgcggg ccgacggagt cctcccagcg gaagccggag acgaagttgc
     1321 cgccggggta gcggatcgtg gtcgagccga gctcgcgcac caggtcgacg acgtccaggc
     1381 ggaagccgtc ctcgttggcg gtcgggtggc cgggctcgta gatgccgtcg tagacgcagc
     1441 ggccgaggtg ctcgacgaag gagccgaaga tgcgccgatc gatcggggcg acgaccgcct
     1501 cgcggtcgat ggagatgcgt gcctgggtca cggtgttcct tttctggatg ggtggggtca
     1561 gtggtccgga tgcccggggt cgagcgtcct gagccggcgg tcgatcagcc gtgcggcgat
     1621 cccgagcggg agcgacgctc ctgccagcgg gacgaagacg ggcagcacgg cggcgatgat
     1681 gccgcaggac acgagggcga gcaccactcc cgtggcggtg aggggtgcgg tcagggcgat
     1741 gccgagcgcg tagcgcagaa ccgccgccgg gcggtcgtcg tagcgggcca gcagcgtgac
     1801 ggcgacgacg ccggtgagca cgacgagcac
//
LOCUS       DATMSX010000369         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_63060,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000369 DATMSX010000000
VERSION     DATMSX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63060"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VN041_11370"
     CDS             <1..1806
                     /locus_tag="VN041_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HWU29673.1"
                     /translation="TSFAETGANRTDKALSCALRGILDSRRDEVALARDALSDVVRNG
                     RNSVFGYVTSGDGFYTDGSFVQHTYLPYVGTYGVVTLGGIAEILGLLGGSEWDVVDPK
                     KSVVLDAVEAAYAPFIWNGRMMDTVRGRAVSRQKAPDYVDGAGAITAILLLAQGAGEP
                     YRSRYLSLVKGWLLRSSEKQYGLPSQTVAKSLLVTSVLGDDSVVPASAPVYTKAFGDQ
                     DRLVHHRPNFSAVVNVSSKRIGRYEWGNRENNLGWYQGDGLTYFYTPVAPDQFSADFW
                     PTVDPYRLPGTTVTAEPRANGANDGTGIPRAYQAFGGGLALDDRWGIQGMDHLNFNKT
                     LSARKSWFFLDDKVVCLGAAVTSASGHEVFTTIDNRSFAPDSVPTLRTDNRYRTVAAG
                     SAAITVRRNLHIDGHGGYVFLKGENVSGSIDVQVVRRTGDWKTINSGSDTGGTDEAKT
                     RDYVTITHHHGTDPSGGGYAYIVLPNVGHSVTFDESETPTVEVVANNAQTQMIRVAGS
                     GLTLANFFAAASADGIQASGPCSVAVRTDAGRTTVALSDPSRTQSVARVTVPNAAGAS
                     IVEADAGVRVVSTAPLTLEFDLDGHGHAKRVVLGA"
BASE COUNT          292 a          667 c          627 g          255 t
ORIGIN      
        1 accagcttcg ccgagaccgg cgcgaaccgc acggacaagg cgctttcgtg cgcgctgcgc
       61 ggcatcctcg actcccgccg cgacgaggtc gcgctcgccc gcgacgcact cagcgacgtc
      121 gtgcgcaacg gccgcaacag cgtgttcggg tacgtcacca gcggcgacgg cttctacacc
      181 gacggctcgt tcgtccagca cacctacctg ccctacgtcg gcacctacgg cgtggtcacg
      241 ctcggcggca tcgccgagat cctcggcctg ctcggcggca gcgagtggga cgtcgtcgat
      301 ccgaagaagt ccgtggtcct ggatgccgtg gaggccgcct acgcgccgtt catctggaac
      361 ggccgcatga tggacacggt ccgcggacgc gcggtgtcgc gccagaaggc cccggactac
      421 gtggacggcg ccggcgccat caccgccatc ctgctgctgg cgcagggggc gggcgagccg
      481 taccgcagcc gttacctgtc gctcgtgaag ggctggctgc tgcgcagcag cgagaagcag
      541 tacggcctgc cgtcgcagac cgtggccaag tcgctgctgg tcacctccgt gctcggcgac
      601 gactcggtgg tcccggcatc cgcgcccgtc tacacgaagg cgttcggcga ccaggaccgg
      661 ctggtgcacc accgcccgaa cttcagcgcg gtcgtgaacg tgtcgtccaa gcggatcggc
      721 cgctacgaat ggggcaaccg cgagaacaac ctgggctggt accagggcga cggcctgacc
      781 tacttctaca cgccggtcgc ccccgaccag ttcagcgcgg acttctggcc caccgtcgac
      841 ccgtaccggc tgccgggcac gacggtcacg gccgagcccc gcgccaacgg cgcgaacgac
      901 ggcaccggca tcccgcgggc gtaccaggcc ttcggcggcg gcctcgccct ggacgaccgc
      961 tggggcatcc agggcatgga ccacctgaac ttcaacaaga cgctcagcgc ccgcaagtcc
     1021 tggttcttcc tcgacgacaa ggtcgtgtgc ctgggagccg cggtcacatc agcatccggc
     1081 cacgaggtgt tcacgaccat cgacaaccga tccttcgcgc cggactccgt gccgacgctg
     1141 cgcaccgaca accgctatcg caccgtggcc gcaggcagtg cggcgatcac ggtgcgccgc
     1201 aacctgcaca tcgacggtca cggcgggtac gtgttcctga agggcgagaa cgtcagcggc
     1261 tcgatcgacg tgcaggtggt gcggcgcacc ggcgactgga agaccatcaa ctccggctcc
     1321 gacaccggcg gcaccgacga ggcgaagacc cgcgactacg tcaccatcac gcaccaccac
     1381 ggcacggacc cgtccggcgg cggctatgcg tacatcgtgc tgccgaacgt cgggcactcg
     1441 gtcacgttcg acgagtccga gacgccgacc gtcgaggtgg tcgccaacaa cgcgcagacg
     1501 cagatgatcc gggtggcggg ctccgggctg acgctggcga acttcttcgc cgcggcgtcg
     1561 gccgacggca tccaggcgag cggcccgtgc tcggtcgcgg tgcggacgga tgccgggcgc
     1621 acgacggtcg cgctctcgga tccgagccgc acgcagagcg tcgcacgggt gacggtcccg
     1681 aacgcggcgg gcgcatcgat cgtggaggcc gatgccggtg tgcgggtcgt ctcgacggcg
     1741 cctctcaccc tggagttcga cctggacggc cacggccacg cgaagcgggt cgtgctcggg
     1801 gcctgacgcc tcacgctcct gcggctcggg ccctcgaggg t
//
LOCUS       DATMSX010000370         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_128974,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000370 DATMSX010000000
VERSION     DATMSX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128974"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VN041_11375"
     CDS             <1..314
                     /locus_tag="VN041_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HWU29674.1"
                     /translation="AAVEAGDTARSVTATAVRYLLQLLDEKAPGNSVEVRVPPFGAVQ
                     VVQGPRHTRGTPPNVVEMNAATWVAVATGAQTWADAVAAGRVSASGVRADLSDLLPLR
                     I"
     gene            385..702
                     /locus_tag="VN041_11380"
     CDS             385..702
                     /locus_tag="VN041_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Trk"
                     /protein_id="HWU29675.1"
                     /translation="MSHSAEHQTTEARIRPVPRFGVFLGLGAVLGVILAGILTAVGSY
                     EKSEVLDIVYPPGQVFGFALLWTVPIGIAVGGLVALILERMARKHERIVRVEHERITD
                     SED"
     gene            complement(721..1419)
                     /locus_tag="VN041_11385"
     CDS             complement(721..1419)
                     /locus_tag="VN041_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWU29676.1"
                     /translation="MKKRTRIILITAGIAVVAIGGAAFAGPLIYRDYLVAPAESAPTL
                     APASPTEVGAAIDPEDLQGDWAVGEGSFAGYRVDEVLNGTDVTVTGRTEKVTGSLTVS
                     DSTLTAARIEVDVASIATDSGNRDSYFRNTAMRAAKFPTATFELTEPVVADGIPQPGV
                     AQKVSAKGELTLAGVTRAVTVELDAVLHGATGQVAGSIPITFSDFGVDAPNLGFVKVE
                     DAGSVEFSLQLVRS"
     gene            complement(1535..2134)
                     /locus_tag="VN041_11390"
     CDS             complement(1535..2134)
                     /locus_tag="VN041_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HWU29677.1"
                     /translation="MSDKPQWLIREDAGTSVLVALALRQLLGIRAPEDLPALRGLEVR
                     RPDATDVDDALEAQWREYWDMTVEPRAHRSEVPLELVDGFDTLVALPTTGAEDLRDAI
                     VPHAAAALRYAQDAHNRYVDSVSSSGGAYRAYASAIAEFEREIGRRAHSFELNVQVLP
                     FSQRGIWWIGALSVAVTDGLRRDVVAFDAAMRPVIAELA"
BASE COUNT          322 a          783 c          801 g          346 t
ORIGIN      
        1 tcgccgcggt cgaggccggc gacaccgcgc gttcggtgac cgcgacggcc gtgcgttacc
       61 tcctccagct gctcgacgag aaggcgccgg gcaacagcgt cgaggtgcgc gtcccgccgt
      121 tcggcgcggt gcaggtcgtg cagggcccac ggcacacccg aggaacgccg ccgaacgtcg
      181 tggagatgaa tgccgcgacc tgggtcgccg tcgccacggg tgcgcagacg tgggcggatg
      241 ccgtggccgc aggccgcgtg tccgcatccg gtgtccgtgc cgatctctcc gacctgcttc
      301 ccctgcgcat ctgacgcccg gccgcactgc cgactgaacg gtgtccggag gccacgcata
      361 tgccgagcgt gggacaatgg aggcatgtcg cacagcgcag agcaccagac gaccgaggcc
      421 cggatccgcc cggtcccgcg cttcggcgtg ttcctggggc tgggcgccgt tctcggcgtg
      481 atcctcgccg gcatcctcac cgctgtcggc agttacgaga agtccgaggt tctcgacatc
      541 gtctacccgc cgggtcaggt gttcggcttc gcactgctgt ggaccgtgcc gatcggcatc
      601 gccgtcggcg ggctcgtcgc gctgatcctg gagcggatgg cgcgcaagca cgagcgcatc
      661 gtgcgggtcg agcacgagcg catcaccgac tccgaggact gagcgtcccg gcgggaccgg
      721 tcagctccgc acgagctgca gcgagaactc caccgagccg gcgtcctcga ccttcacgaa
      781 cccgaggttc ggcgcatcca cgccgaagtc ggagaaggtg atcgggatgc tgcccgccac
      841 ctgtcctgtg gcgccgtgca gcacggcgtc cagctccacc gtcacggcgc gggtgacccc
      901 tgcgagcgtc agctcgccct tcgcgctcac cttctgcgcg acgcccggct gggggatgcc
      961 gtcggcgacc acgggttcgg tcagctcgaa cgtcgcagtg gggaacttcg ccgcgcgcat
     1021 cgccgtgttg cggaagtagc tgtcgcgatt tccggagtcg gtcgcgatcg aggccacgtc
     1081 gacctcgatg cgcgccgcgg tcagtgtgga gtcggacacg gtcagtgagc ccgtgacctt
     1141 ctccgtccgc cccgtgacgg tgacgtcggt gccgttcagc acctcgtcca cgcggtatcc
     1201 ggcgaaagac ccctcgccga cggcccaatc gccctgcagg tcttccgggt cgatggccgc
     1261 ccccacctcg gtgggcgatg cgggggcgag cgtgggtgcc gattcggcgg gggcgacaag
     1321 atagtcgcgg tagatcagcg gcccggcgaa tgcggcaccg ccgatcgcga cgaccgcgat
     1381 gccggcggtg atgaggatga tgcgggtgcg cttcttcatg gcagtgtcct gtcgtggagg
     1441 agtgttcgtg acggtgcaac cttgtcgccg agtccactat gccgctcctc agcgaccgca
     1501 catgatcccg ccaggtgggc cataggaagc gcgctcaggc gagctcagcg atcacgggcc
     1561 gcatggccgc gtcgaaggcg acgacgtcgc gtcgcaggcc gtcggtcacg gcgacgctca
     1621 gcgcgccgat ccaccagatc ccccgctggc tgaacggcag cacctgcacg ttcagctcga
     1681 aggagtgcgc gcgccggccg atctcgcgct cgaactcggc gatcgcgctg gcgtacgcgc
     1741 ggtaggcgcc tccggaggag ctgaccgagt cgacgtagcg gttgtgcgcg tcctgcgcgt
     1801 agcggagagc tgccgcggcg tgcggcacga tcgcgtcgcg cagatcctcc gcaccggtgg
     1861 tgggcagcgc cacgagggtg tcgaagccgt cgaccagctc caggggcacc tcggagcggt
     1921 gcgcgcgcgg ttcgacggtc atgtcccagt actcgcgcca ctgcgcctcg agcgcatcgt
     1981 cgacatcggt cgcgtcgggc cggcgcacct cgagtccgcg cagagcggga agatcctccg
     2041 gcgcgcggat gccgagcagc tggcgcagcg cgagcgcgac cagcaccgag gtgccggcgt
     2101 cctcacggat cagccactgc ggcttgtcgc tcatggcccc atcgtatggg ggttgcgacc
     2161 atggcgcgcc gactcttcgg agggtctccg catgtcggaa tcgcgcaggg gccgcatccg
     2221 tcagacggat gcgacccctg cgcggctgca ac
//
LOCUS       DATMSX010000371         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_119626,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000371 DATMSX010000000
VERSION     DATMSX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119626"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..277
                     /locus_tag="VN041_11395"
     tRNA            205..277
                     /locus_tag="VN041_11395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Ala,seq:cgc)
     gene            363..821
                     /locus_tag="VN041_11400"
     CDS             363..821
                     /locus_tag="VN041_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29678.1"
                     /translation="MDTKNLDNAPVLVAELDRLHADASGAGIDGEIAVWKAATRLGVW
                     FFINRGTDDAVVPYAVQPPGMAPVVCVYSCRERAAEAASALGQREDVVFGLLTPKALE
                     YALALGERGAGGIVLDHPRIGLWTPMANVPRFGAWLVPQSAIRRVSEDEA"
     gene            818..1465
                     /locus_tag="VN041_11405"
     CDS             818..1465
                     /locus_tag="VN041_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29679.1"
                     /translation="MSTEWPGVLDVDALKPGHVAQVDRLADAARAGDWASVLALLDAG
                     HGLTANHSRITGRSWFAPLHQAAWLGAPEQVAEQLLRRGAWRSLRTADGELPSDIARN
                     RGHDHLQRVLELPETREPEQRRFAAWDRHLAALIAERTERLGPVRIRPVQTEVIVRDR
                     LESLWFGYPGMYGGFRLSTHQDRLFVESWSRVVGGSGQAHVITESGCVLVEEGFV"
     gene            complement(1462..>2154)
                     /locus_tag="VN041_11410"
     CDS             complement(1462..>2154)
                     /locus_tag="VN041_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU29680.1"
                     /translation="SQAVDDGVLREREQHRRFLARLDHELKNPVMAIRAQAAAVEPTP
                     EWRTVDAQAAKLSTLVRDLRKLAELESRPLELEQIDLEELVRESVQAIGQQHPDAASR
                     FQVVATTVPWPVPKVRGDLDLLSLALDNVLGNAAKYSAQGAIEVRLREQQGAAVIEVA
                     DNGRGIPAEDLPQVFDELARAGNARDVPGSGIGLTLVATVLRRHGGDVDVRSVADAGT
                     LVTLRIPAAPGV"
BASE COUNT          301 a          743 c          786 g          324 t
ORIGIN      
        1 cgcgccgagc tgcgtcgtcg tgccgaggag ctcgccccgc ccgtcccgac tccgctggtc
       61 gccccggccg cgaaggcgga tgccgcggag tcgccgtacg cgcggatggg cgtcatcgac
      121 gaccgcgaga tcgaggcgca cgtgcgcgag ctgctcgcgc gtcgagcagc cggctgagcg
      181 cgtgatagcc tagatgagtt gtcagggcct atggcgcagt tggtagcgcg cctcgttcgc
      241 atcgaggagg tcaggggttc gaatcccctt aggtccacag cagaaagccc ccggttcgcc
      301 gggggctttc gtcatagctg ccggtcgacc ggaacgatga tgcccgaggg atgaggagga
      361 gtatggacac gaagaatctg gacaacgcac ctgttctggt ggcggagctg gatcgtctgc
      421 acgccgacgc gagcggagcg ggcatcgacg gtgagatcgc cgtctggaag gcagccacgc
      481 gcctcggcgt gtggttcttc atcaaccggg gcacggacga cgcggtcgtt ccctacgccg
      541 tccagccgcc gggcatggcg ccggtcgtct gcgtctacag ctgccgcgaa agggcggcgg
      601 aggctgcctc cgcactcggt cagcgcgagg acgtggtgtt cggcctgctc accccgaagg
      661 ccctcgagta cgcgctcgcc ctgggggaga gaggagccgg cggcatcgtg ctggatcatc
      721 cgcgcatcgg cctgtggacg ccgatggcga acgtcccgcg tttcggcgcg tggctggtgc
      781 ctcagtccgc catcaggcgc gtctccgaag acgaggcatg agtacggagt ggccgggtgt
      841 cctcgacgtc gacgccctga agccgggcca tgtggcgcag gtcgatcggc tcgccgacgc
      901 cgccagggcg ggggactggg catccgtcct cgccctcctg gatgcaggcc acggcctcac
      961 cgccaaccac tcgcggatca ccgggcgatc ctggttcgcg cctctgcacc aggcggcctg
     1021 gctcggggcg cctgagcagg tcgcggagca gctgctgcgg cgcggcgcct ggcggtcgct
     1081 gaggaccgcc gacggagaac tcccgtccga catcgcccgg aacaggggcc acgaccacct
     1141 gcagcgggtc ttggagctgc ccgagaccag ggagccggag cagcgccggt tcgccgcctg
     1201 ggatcgccat ctcgcagccc tgatcgccga gcgcacggag cggctgggcc cggtgcggat
     1261 ccggcccgtg cagaccgagg tcatcgtgcg ggaccggctg gagagcctgt ggttcggcta
     1321 tcccgggatg tacggcggat tccgcctgtc gacccatcag gaccggctgt tcgtggagag
     1381 ctggtcccgc gtggtcggcg gctccggcca ggcgcacgtc atcaccgaga gcggctgcgt
     1441 gctcgtcgag gaggggttcg tctagacccc cggcgccgcc gggatgcgca gggtgaccag
     1501 ggttccggca tccgccaccg atcgcacgtc gacgtcaccg ccgtgccggc gcagcacggt
     1561 cgcgaccagc gtgaggccga taccggagcc gggcacgtcc ctggcgttgc cggcgcgggc
     1621 gagctcgtcg aacacctggg ggaggtcctc ggccggaatt ccgcgtccgt tgtcggccac
     1681 ctcgatcacg gcggcgccct gctgctcccg cagtcgcacc tcgatcgcac cctgggcgga
     1741 gtacttggcg gcgttgccga gcacgttgtc cagggcgagc gagagcagat cgaggtcgcc
     1801 ccgcaccttc ggcacgggcc acggaaccgt ggtggcgacg acctggaagc gcgaggcggc
     1861 gtccggatgc tgctgaccga tcgcctgcac ggactcgcgg accagctcct ccagatcgat
     1921 ctgctcgagc tccagcggcc ggctctccag ctcggcgagc ttgcgcagat cgcgcaccag
     1981 cgtgctgagc ttggccgcct gcgcgtcgac cgtgcgccat tccggggtcg gctcgaccgc
     2041 cgcggcctgc gcgcggatcg ccatcaccgg gttcttcagc tcatggtcga ggcgcgccag
     2101 gaagcgccgg tgctgttccc gttcccgcag cacgccgtcg tccacggcct gaga
//
LOCUS       DATMSX010000372         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166549,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000372 DATMSX010000000
VERSION     DATMSX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166549"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..852
                     /locus_tag="VN041_11415"
     CDS             469..852
                     /locus_tag="VN041_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWU29681.1"
                     /translation="MGTIHRLIEAKKAEREEEGKDAGFSLIELIIVVVILGILVAIAI
                     PIFGNIQNEAKINAAKAAAANAATQWSSQLAAGDTPSAYVTGDTDLTISAAPGAGTNI
                     EDVCATATNATITGGTTAFSSGPGC"
     gene            921..1319
                     /locus_tag="VN041_11420"
     CDS             921..1319
                     /locus_tag="VN041_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWU29682.1"
                     /translation="MSPHTRADDGFGLVEVIIAMVLLAIIAMALLPALWNGIRYSAEQ
                     SSVATATRQVNAVVEQIRGYEKPTCTDIQHELDLAAVAKPDGRNGSITVRLKDGQTLS
                     CTPGNLIDLPLEAMLDGKSIASVHGKVFVP"
     gene            1316..1864
                     /locus_tag="VN041_11425"
     CDS             1316..1864
                     /locus_tag="VN041_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29683.1"
                     /translation="MKLRDEDGLSLIELIITVLIIGVLGIAIGMIFINSVRAQDTVTT
                     TTEATNSGQLYGQSIERAMRNAQAFDVTDSGRMLRVQTSLAGAKQCQAFWITKPSGSS
                     DDALYMTSNASTLPGALTSSWPKPWHEVGGVATGDGGVYFADGGDRVSFSFNIKTDAA
                     PVTITGSAARRFPSTGGANTCW"
     gene            1855..3477
                     /locus_tag="VN041_11430"
     CDS             1855..3477
                     /locus_tag="VN041_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29684.1"
                     /translation="MLVIKRRFSAWARSAVGSDERGAILVAVVVVMLVGFIIAATVAA
                     AVMFTISANADNRSSTQAFIAAESGRDAVVSQISDGCPSAGSMSASSASPAYVANAYP
                     VTSDGQISTTGLTPTCPTADTAYVLIESTGTGPNGDKTTIQAVYRWSATFSSVPGGVV
                     TYFSGNVSQGVSHYTGDMVLRNGNWSCTVDGTLDGDLYVLSGTVSMSNNCTIKGDIWS
                     AGAVSSNSTGWKITANARTGAHGDITTNGYVSFNSNGDPAVAGSISAKGTVTLKDAGS
                     GAGKVGGTVTSMVGATVDTPAWTTGTVTQNTGAAPTFVPPLDWLLQATKWLDLNNTSG
                     WGTITPGDCSKLQNSGTASPYVTGLVGAGTTPLVLDFTGCTKAMDIKLTSSAKLTRDV
                     TIIVPPGQRMLVDLAGTLSDGAHQLFLVHSDASLTDVDADGFPKPTCGNGNQNDSFGT
                     SAVVSSDLKVMVYSPCGLTGTVTASFSGQLYSNDTTNFHAGSSYTCATMSWPGALEKL
                     GCTIKGDDGVGGDSVLVQKLGTRVVQREVPNP"
     gene            3474..4301
                     /locus_tag="VN041_11435"
     CDS             3474..4301
                     /locus_tag="VN041_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HWU29685.1"
                     /translation="MTPAILFITVFAVLFGLAIGSFLNVVAYRVPAGIPLTRESRCPQ
                     CDTGIRWWQNVPVVSWLALRGRCASCGQRISPRYPIIEALTAVLFALVALGLMLPHPT
                     PGMLTDAGFWGILITLAVFAGLSVALTLIDLDVKRLPDAIVLPGWGAIVVLLLVTTLV
                     AHLDWWSFLRALIGGAALFLFYCLIRLISPRGMGGGDVKLAGLVGTVLGWFGWGALAF
                     GAFAAFLLGGIFGGILLLTGRAKRRSAIPFGPWIIAGAWVGIVVGEPIGRWYLGFMS"
     gene            4327..>5104
                     /gene="pilM"
                     /locus_tag="VN041_11440"
     CDS             4327..>5104
                     /gene="pilM"
                     /locus_tag="VN041_11440"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWU29686.1"
                     /translation="MSSTSVGVEITEESVRAVEVSTGRSPRVLAYGEVALPPDAAKDS
                     EIVDEGAVAVALRQLWTGARIKSRSVTLGIASRRVLVREYSTAVMPPDVLRRALPFQV
                     QDLLPVPVNQAVLDYYPTTQQGDQLHGLLVAAVAETVEQLIAAFTRTKIRVQSVDLTA
                     FGLARAARVAAPEGTVAVVHVGDHTTQVVIMRDGIPEFVRILPADLETAAVRRRSALL
                     SITDDGRPISTVTDIANTVDPARLHNPEDAIGAAGIVPRGA"
BASE COUNT          841 a         1752 c         1693 g          818 t
ORIGIN      
        1 gtcgcaccgc ggcgcgggcg atcgcggaca cggatgctcc ggagcgggcg gatgcgcagc
       61 cccgacgtcg ccgcggatgg tggcggcgct cggtgtgacg cggggtcggt tcagccagcg
      121 acgatcagac cggacgtgcc caccgttccc gacgccgacg ggagccagga agatcgcttc
      181 tgccaggtcg cccgtgatga cgcggatggg cgcgctccca cgacggcgag cggagggcgg
      241 tggatgctgg acggcgtcca gttgatctcc gtgaccggac cgtgactttt ggctcttgtg
      301 gagcggatgc tgtgggtcca atatgtgctc aaggagccgc tcaatactgg gatggcggcg
      361 gctccgaact ctggggaccg ggactgacgg tattcgacag gcgctgctgg ggcgcgccgg
      421 cgcacagagg gctttccccc tacccgccgt cccggaggga actctgtcat gggcactatc
      481 caccgcctca tcgaggcaaa gaaggctgag cgcgaggaag aaggcaagga cgccggcttc
      541 tcgctcatcg agctgatcat cgtcgtggtg atcctgggca tcctggtcgc gatcgcgatt
      601 ccgatcttcg ggaacatcca gaacgaagcc aagatcaacg cagcaaaggc tgcggcagcg
      661 aacgcggcca cgcagtggag ctctcagctc gccgccgggg acacgccgtc ggcatacgtg
      721 actggggata ccgacctcac gatcagcgcc gcgcctggag ctggcaccaa catcgaggat
      781 gtctgcgcaa ccgcaacgaa cgcaacgatc accggcggaa cgacggcatt ctcgtccggc
      841 ccgggctgct gatccaatcc tgaccgaagc ggaggccctt cctcgggcct ccgcttcggc
      901 aacctccttt gaggactctc atgtctccgc atacccgagc ggatgacggc ttcggcctgg
      961 tcgaggtcat catcgcgatg gtgctcctgg cgatcatcgc catggcgctg ctgcctgcgc
     1021 tgtggaacgg catccgctat tccgccgagc agtcgagcgt cgcgacggcc acccggcagg
     1081 tgaacgcggt cgtggagcag atccgaggct acgagaagcc cacctgcacg gacatccagc
     1141 acgaactgga tctcgccgct gtcgcgaagc cagacggccg caacggctcg atcacagtgc
     1201 ggctgaagga cgggcagacg ctcagctgca cgccggggaa cctcatcgac ctcccgctcg
     1261 aggcgatgct cgacggcaag agcatcgcga gcgtgcacgg caaggtgttc gtgccatgaa
     1321 gctccgggac gaggacgggc tctcgctcat cgagctgatc atcaccgtgc tgatcatcgg
     1381 cgtgctgggc atcgcgatcg gcatgatctt catcaactcc gtcagagcgc aggacaccgt
     1441 gacgaccacc accgaagcga ccaacagcgg tcagctgtac gggcagagca tcgagcgcgc
     1501 gatgcgcaac gcgcaggcgt tcgacgtgac cgacagcggc cggatgcttc gcgtgcagac
     1561 gagcctcgcc ggtgccaagc agtgccaggc gttctggatc acgaagccgt ccggttcttc
     1621 ggacgatgcg ctgtacatga cgtcgaacgc gtcgacgctg ccgggagcgc tcacctcgtc
     1681 gtggccgaag ccgtggcacg aggtgggggg cgtcgcaaca ggtgacggcg gcgtgtactt
     1741 cgccgacggc ggggatcgcg tgtcgttctc cttcaacatc aagacggatg ctgcgccggt
     1801 gaccatcacg ggttccgcag cgcgtcggtt cccctcgacg ggaggcgcga acacatgctg
     1861 gtgatcaagc gcaggttctc ggcgtgggct cggagcgcgg tggggagcga tgagcgcggc
     1921 gcgatcctcg tcgcggtcgt cgtcgtgatg ctcgtcggct tcatcatcgc ggccacggtc
     1981 gccgcagccg tgatgttcac gatcagcgcg aacgccgaca accgcagcag cacccaggcc
     2041 ttcatcgccg ccgaatccgg gcgggatgcc gtcgtgtccc agatctccga cggctgcccc
     2101 tcggcaggca gcatgtccgc gtcgtccgct tcgcccgcgt acgtcgcgaa cgcatacccc
     2161 gtgacctcgg atgggcagat cagcaccacc ggcctgacgc cgacctgccc gaccgccgac
     2221 accgcatacg tcctcatcga atcgaccggc acgggcccga acggcgacaa gacgacgatc
     2281 caggcggtgt accgctggtc ggcgacattc tcgagcgtcc ccggtggcgt cgtcacgtac
     2341 ttctccggca acgtctctca gggcgtctcg cattacacgg gcgacatggt gctgcggaac
     2401 ggcaactggt cctgcaccgt cgacggcacg ctcgacggcg atctctacgt gctctccggc
     2461 accgtgtcga tgagcaacaa ctgcacgatc aaaggcgaca tctggtcagc cggtgcggtc
     2521 tcgagcaact cgacgggctg gaagatcacc gcaaacgcgc gcaccggagc acacggtgac
     2581 atcaccacca acggctacgt gagcttcaat tccaacggtg atcccgcggt ggccggcagc
     2641 atctccgcga aggggaccgt caccttgaag gacgccggct cgggagcggg gaaagtcggc
     2701 ggaacggtga cctcgatggt cggcgcgact gtggacacac ctgcatggac cacaggaacc
     2761 gtcacgcaga acacgggcgc tgcaccgacc ttcgtccccc cgctcgactg gctgctccag
     2821 gccacaaaat ggctcgacct gaacaacacg tcggggtggg ggaccatcac gccgggcgac
     2881 tgctccaagc tccagaactc gggcacggca tcgccctacg tgacgggact ggtcggcgcc
     2941 gggaccacgc ctctcgtcct cgacttcaca ggatgcacga aggcgatgga catcaagctg
     3001 acctccagcg cgaagctcac ccgcgacgtg acgatcatcg tgccgcccgg gcagcggatg
     3061 ctcgtcgacc tcgccggaac cctgagcgac ggcgcgcatc agctgttcct cgtgcactcc
     3121 gacgccagcc tgaccgacgt cgacgccgat ggcttcccga agccgacctg cggcaacggc
     3181 aatcagaacg acagcttcgg cacatccgcg gtcgtcagca gcgatctcaa ggtgatggtc
     3241 tactcgccct gcggtctgac gggaaccgtc acggccagct tctcgggtca gctctactcg
     3301 aacgacacca ccaacttcca cgccggttcc tcttatacct gcgccacgat gtcgtggccg
     3361 ggtgcgctcg agaagctcgg ctgcacgatc aagggcgatg acggtgtcgg cggcgactcc
     3421 gtgctggttc agaagctggg cacacgagtc gttcagcgtg aggtgcccaa cccatgaccc
     3481 ccgccatcct cttcatcacg gtcttcgcgg tcctgttcgg gctcgcgatc ggatcgttcc
     3541 tgaacgtggt cgcgtaccgc gttccggcgg gcatcccgct gacgcgggag agccggtgcc
     3601 cgcagtgcga caccggcatc cgctggtggc agaacgttcc ggtcgtctcc tggctcgcgc
     3661 tgcggggcag gtgcgcgagc tgcgggcagc gcatctcgcc gcggtacccg atcatcgaag
     3721 ccctgaccgc cgtcctcttc gcgctggtcg cgctcgggct gatgctgccg catccgacgc
     3781 cggggatgct cacggatgcc gggttctggg gcatcctgat caccctcgcc gtcttcgccg
     3841 ggctcagcgt cgcgctcacg ctgatcgacc tggacgtgaa gcggctcccc gatgcgatcg
     3901 tgctgcccgg atggggtgcg atcgtcgtgc tgctgctggt cacgacactg gtcgctcacc
     3961 tcgactggtg gtccttcctc cgtgcgctca tcggcggcgc cgccctcttc ctcttctact
     4021 gcctgatccg cctgatcagc ccccgcggca tgggcggcgg agacgtgaag ctggccggcc
     4081 tggtcggcac ggtgctgggc tggttcggct ggggagccct ggccttcggc gccttcgcgg
     4141 ccttcctgct cggcgggatc ttcggcggca tcctgctgct gaccggccgt gcgaagcgaa
     4201 gatcggccat ccccttcggc ccgtggatca tcgcgggcgc gtgggtgggg atcgtcgtgg
     4261 gcgagccgat aggccgctgg tacctgggat tcatgagctg aggggctcga gaaggggaga
     4321 aggggaatga gcagcacgag tgtcggtgtg gagatcactg aggagagcgt gcgggccgtc
     4381 gaggtgagca ccggcagatc gccgcgtgtg ctcgcgtacg gcgaggtcgc gctgccaccg
     4441 gatgccgcca aggactccga gatcgtcgac gagggcgccg tggcggtcgc gctgcgccag
     4501 ctctggacgg gcgcgcgcat caagagccga tccgtcaccc tgggcatcgc cagtcgccgt
     4561 gtgctcgtgc gcgagtacag caccgcggtg atgccgcccg acgtgctgcg ccgggcgctg
     4621 ccgttccagg tgcaggatct gcttccggtc cccgtcaacc aggccgtgct cgactactac
     4681 ccgaccacac agcagggcga tcagctgcac ggactgctgg tcgccgccgt cgccgagacc
     4741 gtcgagcagc tcatcgccgc gttcacccgc accaagatcc gcgtgcagag cgtcgacctc
     4801 accgccttcg gcctcgcccg cgccgcccgc gtcgccgccc ccgagggcac cgtcgccgtc
     4861 gtccacgtcg gcgaccacac cacgcaggtc gtgatcatgc gcgacggcat ccccgagttc
     4921 gtgcgcatcc ttccggcgga cctcgagacg gccgcggtcc gtcgccgctc ggcgctcctg
     4981 tcgatcaccg acgacggccg cccgatctcg accgtcaccg acatcgcgaa cacggtcgac
     5041 ccggcacggc tgcacaaccc ggaggatgcc atcggcgccg ccggcatcgt tccgcgcggc
     5101 gccc
//
LOCUS       DATMSX010000373         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_91151,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000373 DATMSX010000000
VERSION     DATMSX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91151"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VN041_11445"
     CDS             complement(<1..1628)
                     /locus_tag="VN041_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29687.1"
                     /translation="MADAHLSSPLRTIPAWAVLQRRLFDEIEQARRLFRDRYTLPDGR
                     LRGVAEFEDRDGVDDLYEPFFNWPAFYLLGGPDEVLADSKRAWEGVTAQLTEAGMLTD
                     EYDNGYDWFHQGESLLFFAGICAADPADEAYAARARRFAALFTDPAHGNYDAEHNIIR
                     APHNGALGARPGLDDTVVPYTADRAEMRPYGLPIQGLPGIDTWDDLADPANALHMSEE
                     MHRRAKGDVAVNLAATSLAANTWLYDGDETASAWIRRYVDGWRERAGADGVMFDSIGP
                     DGVAGSLQEGRWWGAHYGWAWPHGFHSVAMSTLIGAVNHALITRDDTALDLVRTMLDA
                     VLAEARPGSAAGSTYSLRGGWNARYGAAAEDEGMLVPYRYGADGWFDFGPLQLDVPVW
                     LWWWSRTDADRARLERAIAGIPASADRVAPFRDKEEAGHEAGWYSFLAGDLPDYPQRA
                     LEMALGQVARRTAMMLGDAQDPDAAHLHFWQRVNPVVTEVLTQLVGGAPQVLYNGGLG
                     FTALRYEDVDRGRPGLPEDVAALVHRLDDRGIGV"
     gene            complement(1631..2530)
                     /locus_tag="VN041_11450"
     CDS             complement(1631..2530)
                     /locus_tag="VN041_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU29688.1"
                     /translation="MATPLTKTPLTKTPLMKQTRNRPSLLAGTARWIVLGVFAITMLI
                     PMYTMVINAFKPQKEIIENPLLITPQSFTFDFLWAAVTSSRFNVIAAYGVTLLFVVLV
                     NLFCILLSAPVAYVIARGRSKWHLGLLLLFVTGLFIPGQVTLIPVVFVLRVLGLINTI
                     PGFILFETAATLPVSIFLFSAYLRTVPRDIDEAASLDGAGPIRAFWTCIFPIMKPVIA
                     TMVVLNSIGVWNDFVSPQIILGPSSGIYTVTTGVYAAVGQFSTDYTQVFPTLLLAVLP
                     ALIFFIVMQRHIIGGLVAGATKG"
     gene            complement(2530..>3539)
                     /locus_tag="VN041_11455"
     CDS             complement(2530..>3539)
                     /locus_tag="VN041_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU29689.1"
                     /translation="GTTRRPTRHSGIEHPELRDMFRTTPALERSPMASTAVPRQRSAA
                     QRWRRRIPGLIMASPAVAVFLAMFVIPMILACVLRFTNWTGYSLNFDFVGWDNYAKAF
                     RNPRSLNAAIFTGVIAVFGTVLCNVIGLAIAALISGPGRANTLLRTIFFYPYVISALI
                     IGFIWSAMLAPQGAVNGMLQGIGLPEMPFLTDPTFAKASIIFTIVWSHFGFNMILFLA
                     GIKSVPTEYYEAATVDGASRWQQFRSITVPLIAPVFTVNIVLTLVGLLKAYDVILALT
                     DGGPAGSTQTIVYQILKDSFANSALGFGAAQSIVLLIVTAVIGLGVTLVRRRADQKAT
                     D"
BASE COUNT          576 a         1121 c         1333 g          509 t
ORIGIN      
        1 tgcacgccga tgccgcggtc gtcgaggcgg tgcacgagcg cggcgacgtc ctccgggagt
       61 ccgggacggc cacggtcgac gtcctcgtag cggagggccg tgaagccgag tccgccgttg
      121 tagagcacct gcggagcgcc gccgacgagc tgcgtgagca cctcggtgac caccgggttc
      181 acgcgctgcc agaagtgcag gtgcgcggca tccgggtcct gcgcgtcgcc gagcatcatg
      241 gccgtgcgcc gggccacctg gccgagcgcc atctccagcg cccgctgcgg gtagtcgggc
      301 agatcgccgg cgaggaagga gtaccagccg gcctcgtgcc cggcctcctc cttgtcgcgg
      361 aacggggcga cgcggtcggc ggatgccggg atgccggcga tcgcgcgctc caggcgcgcc
      421 cggtcggcat ccgtgcgcga ccaccaccac agccagaccg ggacgtccag ctgcagcggc
      481 ccgaagtcga accagccgtc cgcaccgtag cggtagggga ccagcatccc ctcgtcctcg
      541 gccgccgcgc cgtagcgggc gttccagccg ccgcgcaggc tgtacgtgct gcccgcggcg
      601 gagccggggc gcgcttcggc gaggacggcg tcgagcatgg tgcgcaccag gtcgagcgcg
      661 gtgtcgtcgc gggtgatcag ggcgtggttg accgcgccga tgagcgtgct catggcgacc
      721 gagtggaacc cgtgcggcca cgcccagccg tagtgcgcgc cccaccagcg gccctcctgc
      781 agggagccgg cgacgccgtc ggggccgatg ctgtcgaaca tgacgccgtc ggcgccggcg
      841 cgctcgcgcc agccgtcgac gtagcggcgg atccacgcgc tcgcggtctc gtcgccgtcg
      901 tacagccagg tgttggcggc cagcgatgtc gccgcgaggt tcaccgcgac gtcacccttc
      961 gcgcgacggt gcatctcctc ggacatgtgc agcgcgttcg cggggtcggc caggtcgtcc
     1021 cacgtgtcga tgccgggcag gccctggatc ggcaggccgt agggacgcat ctcggcgcga
     1081 tcggcggtgt acggcacgac cgtgtcgtcc aggcccgggc gggccccgag ggcgccgttg
     1141 tgcggcgcgc ggatgatgtt gtgctcggcg tcgtagttgc cgtgcgccgg gtcggtgaac
     1201 agtgctgcga agcggcgcgc gcgtgcggcg taggcctcgt cggcggggtc ggcggcgcag
     1261 atgccggcga agaagagcag ggactcgccc tggtggaacc agtcgtagcc gttgtcgtac
     1321 tcgtcggtga gcatgccggc ctcggtcagc tgcgcggtca cgccctccca ggcccgcttg
     1381 ctgtcggcga gcacctcgtc cggcccgccc agcaggtaga acgccggcca gttgaagaac
     1441 ggctcgtaca ggtcgtcgac cccgtcgcgg tcctcgaact cggcgacacc gcgcaggcgg
     1501 ccgtcgggaa gcgtgtaccg gtcgcggaac agccggcgcg cctgctcgat ctcgtcgaac
     1561 agccggcgct gcagcacggc ccacgcgggg atggtccgca gcggggagga gaggtgcgcg
     1621 tctgccatgg tcagcccttc gtggccccgg cgaccaggcc gccgatgatg tgccgctgca
     1681 tgacgatgaa gaagatcagc gcgggaagca cggccagcag cagcgtcggg aacacctgcg
     1741 tgtagtcggt ggagaactgg ccgaccgcgg cgtacacgcc ggtggtgacc gtgtagatgc
     1801 cggagctcgg ccccaggatg atctgcgggc tgacgaagtc gttccacacg ccgatcgagt
     1861 tcagcaccac catcgtggcg atgacgggct tcatgatcgg gaagatgcag gtccagaacg
     1921 cgcggatggg gcccgcgccg tcgagggagg cggcctcgtc gatgtcgcgg gggacggtgc
     1981 gcaggtaggc cgagaacagg aagatcgaca ccggcagggt ggcggccgtc tcgaacagga
     2041 tgaatccggg gatcgtgttg atcagcccca gcactctgag cacgaacacc accgggatca
     2101 gggtgacctg gccggggatg aacaggccgg tcacgaacag cagcagcagt ccgaggtgcc
     2161 atttcgagcg gccgcgggcg atcacatagg cgaccggggc ggacagcagg atgcagaaca
     2221 ggttgaccag caccacgaac agcagggtca caccgtaagc ggcgatgacg ttgaaccggc
     2281 tgctggtgac ggcagcccac aggaagtcga acgtgaagct ctgcggcgtg atcagcagcg
     2341 ggttctcgat gatctccttc tgcggcttga aggcgttgat caccatcgtg tacatcggga
     2401 tcagcatcgt gatcgcgaag acgcccagga cgatccaccg cgctgtgccg gccaggaggg
     2461 acgggcggtt gcgggtctgc ttcatcagcg gggtcttggt cagcggggtc ttggtcagcg
     2521 gggtggccat cagtcggtcg ccttctggtc tgcgcggcgg cgcacgagcg tcacgccgag
     2581 gccgatcacg gccgtcacga tgaggagcac gatcgactgg gcggcgccga agccgagcgc
     2641 ggagttcgcg aaggagtcct tcaggatctg gtagacgatg gtctgcgtgg atccggcggg
     2701 gccgccgtcg gtcagggcca ggatcacgtc gtacgccttc agcaggccga ccagggtcag
     2761 cacgatgttc acggtgaaga ccggggcgat gagcggcacg gtgatcgaac ggaactgctg
     2821 ccagcggctg gcgccgtcca cggtggcggc ctcgtagtac tcggtgggca ccgacttgat
     2881 gccggcgagg aacaggatca tgttgaagcc gaagtgcgac cacacgatcg tgaagatgat
     2941 gctcgccttc gcgaaggtcg ggtcggtgag gaacggcatc tccggaaggc cgatgccctg
     3001 cagcatcccg ttcacggcgc cctgcggcgc gagcatcgcg gaccagatga agccgatgat
     3061 cagcgcgctg atcacgtacg ggtagaagaa gatcgtgcgc agcagcgtgt tcgcacgtcc
     3121 cggccccgag atcagggcgg cgatggcgag cccgatgacg ttgcagagca cggtgccgaa
     3181 cacggcgatg acgccggtga agatcgcggc gttcagggag cgcgggttgc ggaacgcctt
     3241 ggcgtagttg tcccagccga cgaagtcgaa gttcaggctg tagccggtcc agttcgtgaa
     3301 cctcagcacg caggcgagga tcatcgggat cacgaacatc gcgaggaaga ccgcgacggc
     3361 gggggaggcc atgatcaggc cggggatgcg gcgccgccag cgctgcgccg cgctgcgctg
     3421 ccgcgggacg gcggtggatg ccatggggga acgctccaat gcgggcgtgg tgcggaacat
     3481 gtctctcagc tcaggatgct cgatgccgga gtggcgggtg ggacgacgcg tcgtcccac
//
LOCUS       DATMSX010000374         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_6179,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000374 DATMSX010000000
VERSION     DATMSX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6179"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VN041_11460"
     CDS             <1..289
                     /locus_tag="VN041_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29690.1"
                     /translation="LPGGEVVGVVGWAGLVVLATGVLVVAGNWSPSTRWTDAVRAVPD
                     TVRADAAGTLYLAATAVFVGVVTWALVPLFIPAIGCAALAVVAVPSRRRRR"
     gene            345..1634
                     /locus_tag="VN041_11465"
     CDS             345..1634
                     /locus_tag="VN041_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU29691.1"
                     /translation="MMSSLARRGRALGAAVALVVGGLALAGCGAAAPEAKFDPDAKVS
                     LDFAFWGNDDRADRYNQLIEAFNEEHPNIQVKTTFSDYPSYWEKRATEAASGSLPDVF
                     QFSDSYLRQYAESGHVLDLSTVKKYIDFSAFDGSLLGTGRLDGTQYSLPTGYSVWANF
                     VNDDLLAQYGVASPEGGSSPAELDDWMAAVTKASGGKVYGGTDYTQRIQGFELELRAH
                     GENLYTQDGKLGFTKKQLKEYWAGGAKLRDGVAVPQQRLEEVSPKSGLGVQLTASEMS
                     WSNFLGGYLADSGAKSISIVAPPAAVEGAKDLYQQGGLQIAIAPNSKHKEAAAMFLDF
                     IVNSEQAGRIFGTTLGFPASSSKLAGTKLEGVDKQVADYIESVSDRIGEAPPVPVVGY
                     GSLEQKFWDLGKEIGLGTVTVDDAVDQFFAEADVVLQ"
     gene            1740..2741
                     /locus_tag="VN041_11470"
     CDS             1740..2741
                     /locus_tag="VN041_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29692.1"
                     /translation="MAVGVKDVAAAAGVSVGTVSNVLNQPDRVSARTAERVQQAIREL
                     GFVRNDAARQLRAGRSRSIGLVVPDIGNPFFADVARGAEDRAAEAGMTVLLGNSDESD
                     VRQEAHLELFQEQRVNGVLLTPASDDLSSLRRFTDAGMPVVLVDHEVDEGEIPSVSVD
                     DVEGGRLAVRHLLDAGRRRVAYVSGPRSVRQVAERLEGAQAAIAEVAGATLEVIEQAS
                     LTVLQGRAAGEALAARAEQDRPDAVFAANDLLAVGLLQAFSFAAGVRVPEDIAMVGYD
                     DIDFASATVVPLSSVRQPARLLGRTGVELLLQELDGAEHDRRVRFQPELVVRDSSRG"
     gene            complement(2757..3497)
                     /locus_tag="VN041_11475"
     CDS             complement(2757..3497)
                     /locus_tag="VN041_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29693.1"
                     /translation="MSIDPTRPEEFAVGDAKQVIRNTLMTTGCLLIVAGVLLWFLIGV
                     LLGMAGVGKPFGMILSILITLAIVALLLWLRAAQLRRNLSGAKLVAGPGGLEQHDDWG
                     NVRFVAWNDIVEAGTVRPLKKDAKMVAGNPMAKAVGEAGVNALAAAAQSLGSAVGILG
                     YGTVDFRRNGSPATRKAYADAFGAGPDGRALVGIQPSVFDADWQDGRVGEWIGHFRPD
                     ILAQARAEISRRNAKKGDTEGDAPSPAS"
     gene            3644..4735
                     /locus_tag="VN041_11480"
     CDS             3644..4735
                     /locus_tag="VN041_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29694.1"
                     /translation="MFRGISLLVLAVLAVCAVGCAADAVQEAESSQSPEPTAPPRPDR
                     SATNWKIGSVRLINPCNALPADEVSARLGNPGAAVNVIEHGFDAPVTEDAYEALTDED
                     KTLSCWLTPPWGDVHFEATLAGREADRRGEYTATGSSSGIHGISAGYAWSLEIEARGA
                     FATEADQKSATLALADTIEKNLEDPALSQRPSWDPADGADPCGVLDYDTAQSVLDGDK
                     QLIPEVKALEPMYRRSYTHRPDSTSPAAKSCGREGPFVSSVDIPKPSHAVIDVVLSQQ
                     SVLKFETGGDGEPVSGLGTEAMWKGDEQASRGSLLIRTGHGYATLEVSYGLAYWYDKK
                     NEVHRPPVPTAAQLTPLAKTLLARFPEER"
     gene            complement(4809..5012)
                     /locus_tag="VN041_11485"
     CDS             complement(4809..5012)
                     /locus_tag="VN041_11485"
                     /inference="COORDINATES: protein motif:HMM:NF044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29695.1"
                     /translation="MLALESVDFGADVAEAVLAADPVEVDGRVSAEVRRRDTGESADV
                     ALVPYHSWAQRGPSTMRVWLPTS"
BASE COUNT          788 a         1725 c         1818 g          776 t
ORIGIN      
        1 cctgcccggc ggcgaagtgg tcggcgtggt gggctgggcc ggcctcgtgg tcctcgcgac
       61 gggtgtgctg gtcgtcgccg gtaactggtc gccttcgacg cgatggacgg atgctgtgcg
      121 cgccgtgccc gacacggtgc gggcggatgc cgccggcact ctctacctgg cggcgacggc
      181 cgtgttcgtc ggcgtggtca cgtgggcgct ggtgccgctg ttcatccccg ccatcggctg
      241 tgcggccttg gcggtcgtcg cggtgccctc gcggcgtcgt cgccgctgac ctcccccgcg
      301 gaatcccgcg gaatccctgc agtcccatcc gaaaggtcct cctcatgatg agctcactcg
      361 cacgacgtgg tcgagcgctc ggtgccgccg tggcgctcgt cgtcggcggc ctggcgctcg
      421 ccggctgcgg cgccgccgcg cccgaggcga agttcgaccc cgatgcgaag gtctccctcg
      481 acttcgcgtt ctggggcaac gacgaccgcg ccgaccgtta caaccagctg atcgaggcgt
      541 tcaacgagga gcatccgaac atccaggtca agacgacctt cagcgactac cccagctact
      601 gggagaagcg cgccaccgag gctgccagcg gcagccttcc ggatgtgttc cagttctcgg
      661 acagctacct gcgccagtac gccgagagcg ggcatgtgct cgacctgtcg acggtgaaga
      721 agtacatcga cttctccgct ttcgacggct ccctgctggg caccggccgt ctggacggga
      781 cgcagtactc gctgcccacc ggctacagcg tgtgggcgaa cttcgtcaac gacgatctcc
      841 tcgcgcagta cggagtcgcg tcaccggaag gcggctccag ccccgctgag ctcgacgact
      901 ggatggcggc ggtgacgaag gcatccggcg gcaaggtcta cggcggcacg gattacacgc
      961 agcgcatcca gggcttcgag ctggaactcc gtgcccacgg tgagaacctc tacacgcagg
     1021 acgggaagct gggcttcacg aagaagcagc tcaaggagta ctgggccggc ggcgcgaagc
     1081 tgcgcgacgg cgtggccgtg ccgcagcagc ggctggagga ggtcagcccg aagtccggcc
     1141 tgggcgtgca gctgaccgcg agcgagatga gctggagcaa cttcctgggc gggtacctgg
     1201 ccgattccgg ggcgaagtcc atttcgatcg tcgcgccgcc ggccgcggtc gagggtgcga
     1261 aggacctgta ccagcagggc ggcctgcaga tcgcgatcgc accgaactcc aagcacaagg
     1321 aggcggccgc gatgttcctg gacttcatcg tcaacagcga gcaggccggg cggatcttcg
     1381 gcacgacgct gggtttcccc gcctcttcga gcaagctcgc cggaacgaag ctggagggcg
     1441 tggacaagca ggtcgcggac tacatcgaat ccgtctcgga ccggatcggc gaggcgcccc
     1501 cggtgcccgt ggtcggctac ggctcgctgg agcagaagtt ctgggatctg ggcaaggaga
     1561 tcggactcgg caccgtcacg gtcgacgacg cggtggacca gttcttcgct gaggcggacg
     1621 tcgtcctgca gtgaaccctg cggctcccgc gccccgtcgg cgcgggagcc gggccgcagg
     1681 atgagaaggt ggattccagc acgcctcctt cggaggccgg tctgaggagg ggagcaccca
     1741 tggcagtggg cgtgaaggat gtcgcggccg cggccggagt gtctgtgggc acggtctcca
     1801 acgtgctgaa ccagccggac cgcgtctccg cgcgcaccgc cgagcgcgtg cagcaggcga
     1861 tccgggaact cggattcgtg cgcaacgatg ccgctcgcca gctccgtgcc ggtcgcagcc
     1921 gcagcatcgg gctggtcgtt cccgacatcg ggaacccgtt cttcgccgat gtggcgcggg
     1981 gcgcggagga ccgggcggcc gaggcaggca tgaccgtgct gctgggcaac agcgacgaga
     2041 gcgacgtccg gcaggaggcg cacctggagc tgttccagga gcagcgcgtg aacggggtgc
     2101 tgctgacccc ggcatccgac gacctgtcgt cgctgcgtcg cttcacggat gccgggatgc
     2161 cggtcgtgct cgtggaccat gaggtcgacg agggcgagat cccctccgtg tcggtcgacg
     2221 acgtcgaggg cggccgcctc gcggtcaggc acctgctcga cgcggggcgg cgccgggtcg
     2281 cctatgtgag cggaccgcgc tccgtgcgcc aggtcgccga gcgactggag ggtgcgcagg
     2341 ccgccatcgc ggaggttgcg ggtgcgaccc tcgaggtcat cgagcaggcc tcactgaccg
     2401 tgctgcaggg gagggccgca ggcgaggcgc tcgccgcgcg tgcagagcag gaccggccgg
     2461 atgccgtgtt cgccgccaac gacctcctcg ccgtcggact gctgcaggca ttcagcttcg
     2521 ccgccggcgt tcgcgtgccc gaggacatcg cgatggtcgg ctatgacgac atcgacttcg
     2581 cctcggcgac ggtcgtcccg ctcagctcgg tgcggcagcc ggcccggctg ctgggcagga
     2641 cgggtgtcga gctgctgctc caggaactcg acggcgccga gcacgaccgc cgcgtgcggt
     2701 tccagcccga gctcgtcgtg cgggactcca gccggggctg atctcctcgg agccgttcag
     2761 ctcgccggag acggagcatc gccttccgtg tcacccttct tcgcgttccg gcggctgatc
     2821 tcggcgcgcg cctgcgcgag gatgtccggg cggaagtgac cgatccactc gcccacgcgt
     2881 ccgtcctgcc agtcggcgtc gaagacggag ggttgaatgc cgacgagcgc ccggccgtcg
     2941 gggccggcgc cgaaagcatc cgcatacgcc ttcctggtcg caggactgcc gttgcggcgg
     3001 aagtcgaccg tgccgtatcc gaggatgccg accgcggagc cgagcgactg cgccgcggcg
     3061 gccagcgcgt tgacaccggc ttctccgacg gccttggcca tggggtttcc ggcgaccatc
     3121 ttggcatcct tcttcagcgg cctcaccgtg ccggcctcga cgatgtcgtt ccaggcgacg
     3181 aagcggacgt tgccccagtc gtcgtgctgc tccaggcccc cgggacccgc cacgagcttg
     3241 gcgcctgaca ggttcctgcg cagctgagcc gcgcggagcc agaggagcag cgcgacgatc
     3301 gcgagggtga tgaggatgct gaggatcatg ccgaagggct tccccacgcc ggccatgccc
     3361 agaagcaccc cgatgaggaa ccagagcagc acccccgcga cgatcagcag gcagcccgtg
     3421 gtcatgagcg tgttccggat cacctgcttc gcgtcgccga cggcgaactc ttcggggcgg
     3481 gtggggtcga tggacatctc gagctccttc ggtttcgatg tcagtcacgt cggtgtcccg
     3541 cagacgctag tcgcccgccg tcgactccgg aatgggcagt tctctccgcc ggaaagcagg
     3601 gtccggtctc gatggcgggg tcgtcgaaag ctaccctgaa cccatgtttc gcggaatatc
     3661 gctcctcgtc ctggcggtgc tggccgtctg cgccgtcggg tgcgcagccg acgcggtaca
     3721 ggaggcagag agctcccaga gcccggaacc gaccgcgccg ccgcgaccgg atcgatccgc
     3781 caccaactgg aagatcggca gcgtccggct gatcaatccc tgcaacgccc tccccgccga
     3841 tgaggtctcc gcccgtctgg gtaacccggg agcagcggtg aacgtcatcg agcatgggtt
     3901 cgatgcgccg gtcaccgagg acgcgtacga ggcgctcacc gacgaggaca agaccctgag
     3961 ctgctggctc accccgccct ggggagacgt gcacttcgag gcgaccctgg ccggccgcga
     4021 ggcggaccga cgcggtgagt acaccgcgac gggctcgagt tcggggatcc acggcatctc
     4081 ggcgggctat gcctggagcc tggagatcga ggccaggggg gcgttcgcga ccgaggccga
     4141 tcagaagtcg gcgacgctcg cgctcgccga cacgatcgag aagaacctcg aggaccctgc
     4201 cctgagccag cggccctcgt gggatccggc cgacggtgcg gacccgtgcg gcgtgctcga
     4261 ctacgacacc gcccagtccg tgctcgacgg ggacaagcag ctcattcccg aggtgaaggc
     4321 gctggaaccc atgtaccggc gttcgtacac gcatcgaccg gacagcacgt ctccagcggc
     4381 caagagctgc gggcgcgagg ggccgttcgt gtcatcggtc gacatcccga agccttcgca
     4441 cgcggtcatc gacgtcgtgc tgtcgcagca gtccgtgctg aagttcgaga ccggcggtga
     4501 cggtgagccc gtctccgggc tcgggacgga ggcgatgtgg aagggcgatg agcaggcgag
     4561 tcgcgggtcg ctgctcatcc ggaccgggca cggctatgcc acgctcgagg tgtcgtacgg
     4621 actcgcctat tggtacgaca agaagaacga agtgcaccgg cctccggtgc ccaccgcggc
     4681 tcagctgaca cccctggcga agaccctgct cgcgcggttc ccggaggagc gttgacgcgc
     4741 tgacgtgccg tagtcgggtg ctcatgccgc tggatcccgc tcacggagat gccgcggacg
     4801 atcgtggctc aactggtcgg caaccacaca cgcatggtcg acgggccgcg ctgcgcccag
     4861 gagtggtagg ggacgagcgc gacgtcggcg gactcgcccg tgtcccgacg ccgcacttct
     4921 gcgctgacgc gtccgtcgac ctcgacggga tccgctgcga gaaccgcctc ggcgacgtcg
     4981 gcgccgaagt ccaccgactc cagagccagc acctccgggc cgcgcagccg ctacgtcggc
     5041 gcagaggtgc ccgcggagga gctcatctgg caggacaacg taccggccgt ggaccacgcg
     5101 ctgatcg
//
LOCUS       DATMSX010000375         7312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_119716,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000375 DATMSX010000000
VERSION     DATMSX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119716"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1153)
                     /locus_tag="VN041_11490"
     CDS             complement(29..1153)
                     /locus_tag="VN041_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HWU29696.1"
                     /translation="MPVSSATTESPTPAGPRDVVLARPLRVLAWLSFLAEVLIIGTGG
                     AVRLTGSGLGCSDWPLCTPESLVPILEVQGIHGAIEFGNRTMTGLVGLLALGVLMLTL
                     HAVSGKRLVIRAVWFAVGGIVLAVLVYIAASALSVVASAALMSAALLLAVLVGMVDSL
                     RRTTVRRDLAVLAWLVLVGVMAQAVVGGFAVISELNPFIVGFHYASSLLLVCVTALFL
                     VRLDAVPGPRERAVPAWFAALTHITGLALVLTILFGVLTTGSGPHSGDVNIKREGFDA
                     SILAHVHSWPGYVLAALVLALAISAWLLALPPRRWLLVLIIAILVQVAVGVLQARTGL
                     PPVLVGVHMLLASLSAAAYTVVVTKLKKPAAGAANPYAED"
     gene            1390..2808
                     /gene="sufB"
                     /locus_tag="VN041_11495"
     CDS             1390..2808
                     /gene="sufB"
                     /locus_tag="VN041_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWU29697.1"
                     /translation="MSDVLIDRPELDGLGVYEFGWHDPDAAGASARRGLSEAVVRDIS
                     ALKSEPEWMLKTRLKGLALFGRKPMPTWGADLSDIDFDHIKYFVRSTEKQAQSWEDLP
                     EDIRETYERLGIPEAERQRLVAGVAAQYESEVVYHQIREDLEAQGVIFMDTDTALREH
                     PEFFEEYFGTVIPAGDNKFAALNTAVWSGGSFVYVPKGVHVEIPLQAYFRINTENMGQ
                     FERTLIIADEDSYVHYIEGCTAPIYKSDSLHSAVVEIIVKKNARVRYTTIQNWSNNVY
                     NLVTKRAVAHEGATMEWVDGNIGSKVTMKYPSIYLVGEHAKGETLSVAFAGPGQHQDA
                     GAKMVHQAPYTQSSIVSKSIARGGGRSGYRGEVRVDPAAHHSSNSVVCDALLVDTQSR
                     SDTYPSIDIRVDDVQLGHEATVSKVSAEQLFYLQSRGISETEAMAMIVRGFIEPIARE
                     LPMEYAMELNKLIEMGMEGSVG"
     gene            2810..4006
                     /gene="sufD"
                     /locus_tag="VN041_11500"
     CDS             2810..4006
                     /gene="sufD"
                     /locus_tag="VN041_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWU29698.1"
                     /translation="MTAPTQAPAEAPHTNAHIDPAARIANTGFVPVQTRSERPHSFEP
                     ADFGAPTGREVNWKHTPIAQLTSLFEPAVANDGATYSVSHEQYLRPALARGTAPRGEF
                     FRPEDVVSAVAWQGTTDAMHLSIPAEEVVAEPIVIQIDGQGGRADAHLVIEALPHSSA
                     TVVLRHAGTAQYAQNVEIIVRDGAKLTLVSVQQWQDDAIHAAAHQARVDRDAELTHFI
                     VSFGGGVVRVNPNVELAGSGAEGRLYGLSFADAGQHLESQVYLHHKGPHTTGDVLYKG
                     ALRGASAHSVWIGDVLIGPDATGTDSYEANRNLVLTEGARADSIPNLEIETGDIVGAG
                     HASATGRFDDEQLFYLQARGITEAEARRLVVLGFLTEVVQRIQIPELQAELLAAIDAE
                     LIGVEA"
     gene            4003..4329
                     /locus_tag="VN041_11505"
     CDS             4003..4329
                     /locus_tag="VN041_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HWU29699.1"
                     /translation="MTALRACGVSELELDTPLRIELDGVAMAIVKDAAGDIHAIGDRC
                     THGDISLAEGFVEGDTLECWAHGSRFSLLSGKPLNLPAYEPVPVYVVEIDGDDVLIDP
                     TVIKEV"
     gene            4330..5100
                     /gene="sufC"
                     /locus_tag="VN041_11510"
     CDS             4330..5100
                     /gene="sufC"
                     /locus_tag="VN041_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWU29700.1"
                     /translation="MSVLEIRDLHVTVETDQGTTPILNGIDLTIKTGETHAIMGPNGS
                     GKSTLAYTIAGHPKYTVTQGTITFDGEDVLSMSVDERARAGVFLAMQYPVEIPGVTVT
                     NFLRTAKTALDGEAPSIRQWTKDVKASMTNLRMDPKFAQRNVNEGFSGGEKKRHEILQ
                     LEVLKPKLAVLDETDSGLDVDALKIVSEGVNRAKEATNLGVLLITHYTRILRYIRPQF
                     VHVVVKGKIVEEGGPELADRLEEEGYDRFLDPSAPIEA"
     gene            5110..5439
                     /locus_tag="VN041_11515"
     CDS             5110..5439
                     /locus_tag="VN041_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWU29701.1"
                     /translation="MTATLTDEKYDEVTEALKDVMDPELGINVVDLGLIYDLAWDDEN
                     DALVIHMTLTSAGCPLTDVLEEQTAQALDSVVDRFRINWVWMPPWGPERITDDGRDMM
                     RALGFAI"
     gene            5508..6698
                     /locus_tag="VN041_11520"
     CDS             5508..6698
                     /locus_tag="VN041_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU29702.1"
                     /translation="MTVTPIRALTLDQLRERSSEKWREYPEDVLPLFVAETDFPLAPA
                     ITAALERAVRIGDTGYVASHTPLAAAYAAFAQRRFGWTVDTARIRSTADVSMGIVEIL
                     RRVIHPGERVIVNPPIYPPFFDLVDEAGGVAERVPLRDTGEGWELDLEAIEKALADGS
                     RAVLLCNPHNPTGSVHSAESLDALARLAERYGATVVSDEIHAPLTQPGVVFTPFLASG
                     DAAQRIGYAVTSASKAFNLAGLKCALMISASDDTTAVLRSLPIEVEWRTGQFGMLAAV
                     AAFSEESDEWLDGLLAALDENRRLLADLLERHLPGARYRMPDAGYLAWVDLTDLGWGP
                     NPAKQILRQAKVALHFGPAFGAQGAGHVRVNFGTSPEILTEAIERIARARTTIDEAVS
                     AVRA"
     gene            6695..>7312
                     /locus_tag="VN041_11525"
     CDS             6695..>7312
                     /locus_tag="VN041_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29703.1"
                     /translation="MSAAAEPTIWDRSRLGITVGTVVLIFLAAIESLAVTTVMPIVAR
                     DLDGEALFAVAFSATISTGVIGMVAVGAWSDRSGPRAPLYTAVALFAAGLLVSGFSSD
                     MHTFILGRLIQGLGAGGQTVALYVVVARVYPPQLHGRIFAAYAAAWVVPSMIGPFLAG
                     AVTEYLHWRWTFLGVAVLTAAAFALVVSRLRGVSLDADAGSDADGV"
BASE COUNT         1217 a         2539 c         2508 g         1048 t
ORIGIN      
        1 gccgggcctc ttcgcatcgg cggggagctc agtcctccgc gtacgggttc gcggccccgg
       61 cggcgggctt cttgagcttc gtcacgacga ccgtgtaggc ggccgcggac agtgaggcga
      121 gcagcatgtg cacgccgacg agcacgggcg gcagcccggt gcgcgcctgc agcacgccga
      181 cggccacctg caccaggatc gcgatgatga gcaccagcag ccagcgccgc ggcggcagcg
      241 cgaggagcca cgccgagatc gcgagcgcga gcaccagcgc ggcgagcacg tagccgggcc
      301 aggagtgcac atgcgcgagg atgctggcgt cgaagccctc ccgcttgatg ttcacgtcgc
      361 ccgagtgagg accggatccc gtggtgagca cgccgaagag gatggtcagc acgagcgcga
      421 ggccggtgat gtgggtgagc gcggcgaacc aggccggcac ggccctctcg cgcgggccgg
      481 gaacggcgtc gaggcgcacc aggaacagcg cggtcacgca cacgagcagg agcgaggagg
      541 cgtagtggaa tccgacgatg aacgggttca gctcgctgat cacggcgaag ccgcccacca
      601 cggcctgcgc catcactccg acgagcacca gccacgcgag caccgccagg tccctgcgga
      661 cggtggtgcg gcgcagcgag tccaccatgc cgaccagcac ggccagcagc agggcggccg
      721 acatcagcgc ggccgaggcc acgacgctca gagcggaagc cgcgatgtag acgaggacgg
      781 ccagcacgat gccgccgacc gcgaaccaca ccgcgcggat gacgaggcgc ttgccgctca
      841 cggcgtgcag agtgagcatg agcacgccga gggcgagcag accgaccagg cccgtcatgg
      901 tgcggttgcc gaactcgatc gcgccgtgga tgccctgcac ctcgaggatc ggcaccagcg
      961 actcgggcgt gcagagcggc cagtccgagc atcccagccc cgaaccggtc aggcggaccg
     1021 cgccgccggt gccgatgatc agcacctcgg cgaggaagga gagccacgcc aggacgcgga
     1081 gcggacgtgc gaggacgacg tcgcgaggcc cggcgggcgt gggggattcg gtcgtggcgg
     1141 agctgacggg catatggcct ccggctccgc atcgtggacg gtcgcggccc gtccgcgagg
     1201 cggaacctgt agaatcggga tggttgtgat gggcgcgcag agcgcggcgt ccatcactga
     1261 cagtctaggc gcgagataag cgccggaatg atggggccca ccagcggcat ccgacccccg
     1321 cagactcgac gggggtcagg tgtgtcgggg cggatgacac cgttgcggcc cgtttgagga
     1381 gagtgtgtga tgtcggatgt gctgatcgac cgcccggaac tggatggtct gggggtgtac
     1441 gaattcggct ggcacgatcc cgacgccgcc ggagcgagcg cgagacgcgg cctcagcgaa
     1501 gccgtcgtgc gcgacatctc cgctctgaag tccgagccgg agtggatgct gaagacccgt
     1561 ctcaagggac tcgcgctctt cggccgcaag ccgatgccga cctggggtgc cgacctcagc
     1621 gacatcgact tcgatcacat caagtacttc gtccgctcca ccgagaagca ggcgcagtcc
     1681 tgggaggacc tcccggagga catccgcgag acctacgagc gcctgggcat ccccgaggcc
     1741 gagcgccagc gcctggtcgc cggtgtcgcc gcgcagtacg agtccgaggt cgtctaccac
     1801 cagatccgcg aggacctgga ggcgcagggc gtcatcttca tggacaccga cacggcgctg
     1861 cgcgagcacc ccgagttctt cgaggagtac ttcggcaccg tgatccccgc cggcgacaac
     1921 aagttcgcgg cgctgaacac ggccgtgtgg tcgggcggct cgttcgtgta cgtccccaag
     1981 ggcgtgcacg tggagatccc gctgcaggcg tacttccgca tcaacaccga gaacatgggt
     2041 cagttcgagc ggaccctgat catcgccgac gaggacagct acgtccacta catcgagggc
     2101 tgcacggctc cgatctacaa gtcggactcg ctgcactcgg ccgtcgtcga gatcatcgtg
     2161 aagaagaacg cccgcgtgcg ctacacgacg atccagaact ggtcgaacaa cgtctacaac
     2221 ctcgtgacca agcgcgcggt cgcgcacgag ggcgcgacga tggagtgggt cgacggcaac
     2281 atcggctcca aggtcaccat gaagtacccg tcgatctacc tggtcggcga gcacgccaag
     2341 ggcgagaccc tctccgtcgc cttcgcgggt cccgggcagc accaggacgc cggcgccaag
     2401 atggtgcacc aggcgccgta cacccagtcg tcgatcgtct cgaagtcgat cgcgcgcggc
     2461 ggcggtcgct ccggctaccg cggtgaggtg cgggtcgatc ccgccgccca tcactcctcg
     2521 aactccgtgg tctgcgacgc cctgctggtg gacacgcagt cccgttccga cacgtacccg
     2581 tcgatcgaca tccgtgtcga cgacgtgcag ctcggccacg aggccaccgt ctccaaggtc
     2641 agcgcggagc agctcttcta cctgcagtcg cgcggcatct ccgagaccga ggccatggcc
     2701 atgatcgtgc gcggcttcat cgagcccatc gcgcgcgagc tgcccatgga gtacgcgatg
     2761 gaactgaaca agctcatcga gatgggcatg gaaggcagtg tcggctgaga tgacggcccc
     2821 cactcaggcg cccgcagagg cgccgcacac gaacgcgcac atcgaccccg cagcgaggat
     2881 cgcgaacacc ggtttcgtgc cggtccagac gcgctccgag cgcccgcact ccttcgagcc
     2941 cgccgacttc ggcgcgccca ccggccgcga ggtcaactgg aagcacacgc cgatcgcgca
     3001 gctcacttcg ctgttcgagc ccgctgtggc gaacgacggt gcgacctact cggtcagcca
     3061 tgagcagtac ctgcggcccg cgctggcgcg cggcaccgcg ccccgtggcg agttcttccg
     3121 cccggaggac gtcgtctcgg ccgtggcctg gcaggggacg acggatgcca tgcatctgag
     3181 catccccgct gaggaggtcg tcgccgagcc gatcgtcatc cagatcgacg gccagggcgg
     3241 ccgtgcggac gcgcacctcg tgatcgaggc gctcccgcac agctcggcga cggtcgtgct
     3301 gcggcacgcc ggcaccgcgc agtacgcgca gaacgtcgag atcatcgtcc gcgacggcgc
     3361 caagctcacc ctggtgtccg tccagcagtg gcaggacgac gccatccacg ccgccgctca
     3421 ccaggcccgc gtggaccgcg acgccgagct cacccacttc atcgtcagct tcggcggcgg
     3481 cgtggtgcgc gtgaacccga acgtcgagct cgcaggcagc ggcgcggagg ggcgcctgta
     3541 cggcctctcc ttcgcggatg ccggtcagca cctggagagc caggtctacc tgcaccacaa
     3601 gggccctcac accaccggcg acgtgctcta caagggcgcg ctgcggggtg cgagcgcgca
     3661 cagcgtgtgg atcggcgacg tgctgatcgg accggatgcc accggcaccg actcctacga
     3721 ggcgaaccgc aacctggtgc tgaccgaggg cgcacgcgcc gactcgatcc cgaacctcga
     3781 gatcgagacc ggtgacatcg tgggagccgg ccacgcgagt gcgaccggtc gcttcgacga
     3841 cgagcagctg ttctacctgc aggcacgagg catcaccgag gccgaggccc gtcgcctggt
     3901 ggtgctcggg ttcctcaccg aggtcgtgca gcgcatccag atccccgagc tgcaggcaga
     3961 gctgctcgcc gcgatcgacg ccgagctcat cggagtcgag gcgtgaccgc gctgcgcgcc
     4021 tgcggcgtca gcgagctgga gctggacacg ccgctgcgca tcgagctcga cggtgtggcg
     4081 atggcgatcg tgaaggacgc cgcaggcgac atccacgcga tcggcgaccg ctgcacgcac
     4141 ggcgacatct ccctcgccga gggcttcgtc gagggggaca ccctggagtg ctgggcccac
     4201 ggctcgcgct tctcgctgct ctccggcaag cccctcaacc tccccgctta cgagcccgtc
     4261 cccgtctacg tcgtcgagat cgacggcgac gacgtgctca tcgaccccac tgtcattaag
     4321 gaagtctgaa tgtctgttct cgagatccgc gatcttcatg tgacggtcga gaccgatcag
     4381 ggcacgacgc cgatcctcaa cggcatcgac ctgacgatca agaccggcga gacgcacgcc
     4441 atcatgggcc ccaacggctc cggcaagtcg accctcgcgt acacgatcgc cgggcacccg
     4501 aagtacaccg tcacgcaggg cacgatcacg ttcgacggcg aggacgtgct gtccatgagc
     4561 gtcgacgagc gcgcccgtgc cggcgtgttc ctcgccatgc agtacccggt ggagatcccc
     4621 ggtgtcacgg tgacgaactt cctgcgcacc gcgaagaccg cgctggacgg cgaggccccc
     4681 tccatccgcc agtggaccaa ggacgtcaag gcgtcgatga ccaacctgcg catggacccg
     4741 aagttcgcgc agcgcaacgt caacgagggc ttctcgggcg gcgagaagaa gcgccacgag
     4801 atcctgcagc tcgaggtgct caagccgaag ctcgccgtgc tcgacgagac tgactccggc
     4861 ctcgacgtcg acgcgctgaa gatcgtctcc gagggtgtca accgcgccaa ggaggccacc
     4921 aacctcggcg tgctgctgat cacgcactac acccgcatcc tgcgctacat ccgtccgcag
     4981 ttcgtgcacg tggtcgtcaa ggggaagatc gtcgaggagg gcggccccga gctggccgac
     5041 cgcctcgagg aggagggcta cgaccgcttc ctcgacccca gtgcgcccat cgaagcgtag
     5101 gctgagagca tgaccgcaac gctgaccgac gagaagtacg acgaggtcac cgaggctctg
     5161 aaggacgtca tggatcccga gctcgggatc aacgtcgtcg acctgggact gatctacgat
     5221 ctcgcctggg acgacgagaa cgacgccctg gtcatccaca tgacgctcac cagcgccgga
     5281 tgcccgctca ccgacgtgct cgaggagcag acggcgcagg cgcttgactc cgtggtcgac
     5341 cgcttccgca tcaactgggt gtggatgccg ccgtggggcc cggagcggat caccgacgac
     5401 gggcgcgaca tgatgcgcgc cctcggcttc gcgatctgac atcggcaccg aaggggcacg
     5461 gccgcagggc tgtgcccctt cggcatcccg ttcgactgga ggagatcatg accgtcaccc
     5521 cgatccgcgc gctcacgctg gaccagctgc gggagcgctc cagcgagaag tggcgggagt
     5581 acccggagga cgtgctgccg ctgttcgtgg cggagaccga cttcccgctc gcgcccgcga
     5641 tcactgccgc gctggagcgt gccgtgcgca tcggcgacac cgggtacgtc gcctcgcaca
     5701 cgccgctggc cgccgcctat gcggcgttcg cacagcgtcg tttcgggtgg acggtcgaca
     5761 ccgcccgcat ccggtcgacg gcggatgtca gcatgggcat cgtcgagatc ctccgtcggg
     5821 tcatccaccc cggggagcgg gtgatcgtca acccgccgat ctacccgccg ttcttcgacc
     5881 tggtggacga ggccggcggt gtcgccgagc gtgttcctct gcgcgacacc ggggagggct
     5941 gggagctcga cctcgaggcg atcgagaagg ccctcgccga cggcagcagg gcggtgctgc
     6001 tctgcaatcc gcacaatccc accgggtccg tgcactccgc ggagagcctg gacgcactgg
     6061 cgcgcctcgc cgagcggtac ggcgccaccg tggtctccga cgagatccac gccccgctga
     6121 cccagccggg cgtcgtcttc actccgttcc tggccagcgg cgacgccgct cagcgaatcg
     6181 ggtacgccgt caccagcgcc agcaaggcgt tcaacctcgc agggctgaaa tgcgccctga
     6241 tgatctcggc ctccgacgac accaccgccg tgctgcgctc cctgccgatc gaggtggagt
     6301 ggcgcaccgg ccagttcggg atgctggcag ccgtcgccgc gttctccgag gagagcgacg
     6361 agtggttgga cggcctgctc gccgcgctgg acgagaaccg caggctgctg gcggacctgc
     6421 tcgaacggca tctgccggga gcgcgttatc ggatgcccga tgccggctac ctcgcgtggg
     6481 tggacctcac cgacctcggc tggggtccga acccggccaa gcagatcctg cgtcaggcga
     6541 aggtcgccct gcacttcggc ccggcgttcg gcgcacaggg agccgggcac gtgcgcgtga
     6601 acttcggcac gagccctgag atcctcaccg aggcgatcga gcggatcgcc cgagcccgca
     6661 cgacgatcga cgaggccgtc tccgcggtac gcgcgtgagc gccgccgcag agccgacgat
     6721 ctgggatcgc agccgcctcg gcatcacggt cggcacggtc gtgctgatct tcctcgccgc
     6781 gatcgagtcg ctggcggtca ccacggtcat gcccatcgtc gcgcgcgacc tggacggcga
     6841 ggccctgttc gcggtcgcgt tctccgcgac catctcgacg ggtgtgatcg gcatggtcgc
     6901 cgtgggggcg tggagcgaca ggtcgggccc gcgcgcgcct ctgtacacgg ccgtggcgct
     6961 gttcgcggcg ggccttctcg tgtcgggctt ctcgtcggac atgcacacct tcatcctcgg
     7021 gcggctcatc cagggactcg gggcgggtgg ccagaccgtc gccctgtacg tcgtggtcgc
     7081 gcgcgtctac ccgccgcagc tgcacggccg catcttcgcc gcgtatgcgg cggcgtgggt
     7141 cgtgccctcg atgatcgggc cgttcctggc gggtgccgtg accgagtacc tgcactggcg
     7201 gtggacgttc ctcggcgtcg cggtgctcac cgccgccgcg ttcgcccttg tcgtgtcgcg
     7261 actgcggggc gtgtcgctgg acgcggatgc cgggtcggat gcggacggcg tc
//
LOCUS       DATMSX010000376         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_6212,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000376 DATMSX010000000
VERSION     DATMSX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6212"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VN041_11530"
     CDS             complement(<1..392)
                     /locus_tag="VN041_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU29704.1"
                     /translation="MRFYMDHAATTPLRPEAREAWLAAGDVVGNASSTHGAGQDARRV
                     LEESRERAAAALGCEPIEVIFTSGGTESINTALHGLWRSRSEGTDAVVLPEGEHHATM
                     DTVAALAEDGAGVRPVPLDAAGRIPVAR"
     gene            502..2571
                     /gene="glgX"
                     /locus_tag="VN041_11535"
     CDS             502..2571
                     /gene="glgX"
                     /locus_tag="VN041_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWU29705.1"
                     /translation="MPAQSTPSLGGSALDDLGVRLHDGVATLRVWSQNASSVELVVFD
                     ADDLDWETAQVPLDRKPGGVWEVTTELLQPGTRYSLRVDGPHGPGNTFNSETLLLDPY
                     GRGLAQGDGYEEWRSVVIADGFDWGGVPKPNTPMDRTVIYEGHIKGLTKRHPDVTAAL
                     HGTYAGLAHPAMIEYFKSIGVTAIELLPVHAFVPEPRLLEKGLTNYWGYNTLSFFTPH
                     NAYASESARKEGPEAVLAEFKGMVKLLHEAGLEVILDVVYNHTSEEGLGGPRSSLRGI
                     DNALYYRQQPDGSYIDTTGVGNTLNTATDAAARLVLDSLRYWATEMQIDGFRFDLATA
                     LGRDANHEYDREHPLLTAIRTDPVLQDVKIIAEPWDVGLGGWQTGNFPDGWSEWNDRY
                     RDRARNFWLSDIDYARRASAPVGIGGFANRLAGSANTYSEERGPLASINFVTAHDGFT
                     LHDLVAYDVKHNEANGEGNRDGADMNRSFNHGVEGPTDDPTILAQRRKAMRNLLGTLL
                     LSAGVPMITAGDEFGRTQRGNNNAYCQDSPLSWLNWEHEQWQRDLTAHVALLTRLRAE
                     NPALRPSRYARLGEHIPDSSVMDWFDQNGETMEVEQWNDPRNRTLQYVAASVPENGES
                     NRILLIVHGTESPIDVRLPDSIEAATAFVELWSSADDTPQSEQRRHAPGEVLPVPGTS
                     MRLFRVE"
     gene            2647..>3151
                     /locus_tag="VN041_11540"
     CDS             2647..>3151
                     /locus_tag="VN041_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HWU29706.1"
                     /translation="MAARTAQEPAALRSPARIPLRPAAETDAPEGVKAGRIPLFSPTP
                     HVPQGYLPKAFAGEVVPFGVVSFREGHDRIGVHVRLTDPSGGESLHRLTARQDGTDRW
                     VADIALDVEGPWRYRFEGFADDFATWAHAASVKAEAGVDIAVMSALGATLLTSAAAEK
                     DRPAAERT"
BASE COUNT          506 a         1168 c         1046 g          431 t
ORIGIN      
        1 aagcgtgcca ccggaatccg ccccgccgcg tccagcggca cgggacgcac ccctgcgccg
       61 tcctcggcga gagctgcgac ggtgtccatg gtcgcgtgat gctcgccctc gggcagcacc
      121 acggcatccg tcccctcgct ccgggaacgc cacagcccgt gcagcgcggt gttgatcgac
      181 tcggtgccgc ccgaggtgaa gatcacctcg atgggctcgc agcccagagc cgccgcggcg
      241 cgctccctgg actcctccag cacccgccgt gcgtcctgac cggctccatg cgtggacgag
      301 gcgtttccga cgacgtcacc cgccgccagc cacgcctcgc gcgcctccgg gcgcaggggg
      361 gtcgtggccg catgatccat gtagaaccgc atccctccat tgtcgcctcc ccacaacggt
      421 gcgcgggctg cggaaccgag gctcagccga cccggcaccc ggattcacgg ccgcccgccg
      481 cgcacgttta ggctgttcct catgcccgcg cagtccaccc cgtccctcgg cggctccgcc
      541 ctcgatgacc tcggcgtgcg cctgcacgac ggcgtcgcga cgctccgagt ctggtcgcag
      601 aacgcctcct cggtcgaact ggtcgtcttc gacgccgatg acctggactg ggagaccgcc
      661 caggtgccgc tggaccgcaa gcccggaggc gtgtgggagg tcacgaccga gctgctgcag
      721 cccggcacgc gctactcgct gcgggtggac ggcccgcacg gccccggcaa cacgttcaac
      781 tcggagactc tgctgctcga tccctacggt cgcgggctcg cgcaggggga cggctacgag
      841 gagtggcgct ccgtcgtcat cgccgacggc ttcgactggg gcggagttcc caagccgaac
      901 acgccgatgg accgcaccgt gatctacgag gggcacatca agggcctcac caagcggcat
      961 cccgacgtca cggcggcact gcacggcacc tatgccgggc tcgcccaccc ggccatgatc
     1021 gagtacttca agagcatcgg cgtgaccgcg atcgagctgc tcccggtgca cgcgttcgtg
     1081 ccggagccgc ggctgctcga gaagggcctg acgaactact ggggttacaa caccctgagc
     1141 ttcttcacgc cgcacaacgc ctacgccagc gagtccgccc gcaaggaggg cccggaggcc
     1201 gtgctcgccg agttcaaggg catggtcaag ctcctgcacg aggcgggcct ggaggtcatc
     1261 ctcgacgtgg tgtacaacca caccagcgag gagggcctgg gcggcccgcg gtcgagcctg
     1321 cgcggcatcg acaacgcgct ctactaccgc cagcagccgg acggcagcta catcgacacc
     1381 accggcgtcg ggaacactct caacacggcg acggatgccg ctgcgcgcct ggtgctcgat
     1441 tcgctgcggt actgggccac cgagatgcag atcgacggct tccggttcga cctggcgacc
     1501 gccctcggcc gcgacgccaa ccacgagtac gaccgcgagc atccgctgct caccgcgatc
     1561 cgcaccgacc cggtcctgca ggacgtcaag atcatcgccg agccctggga tgtgggcctg
     1621 ggcggttggc agaccggcaa cttccccgac ggctggagcg agtggaacga ccgctaccgc
     1681 gatcgggccc gcaacttctg gctgagcgac atcgactacg cccgtcgcgc ctcggcgccg
     1741 gtgggcatcg gcggcttcgc gaaccggctc gcaggctccg ccaacacgta tagcgaggag
     1801 cgcggcccgc tggcgagcat caacttcgtc accgcgcacg acggcttcac gctgcacgac
     1861 ctggtcgcgt acgacgtgaa gcacaacgag gcgaacggcg agggcaaccg cgacggcgcc
     1921 gacatgaacc gctcgttcaa ccacggcgtc gagggtccga ccgacgaccc gacgatcctc
     1981 gcgcagcgcc gcaaggcgat gcgcaacctg ctgggcaccc tgctgctgtc ggcaggcgtc
     2041 ccgatgatca ccgccggtga cgagttcggg cgtacgcagc gcggcaacaa caacgcgtac
     2101 tgccaggact cgccgctgag ctggctgaac tgggagcacg agcagtggca gcgcgatctc
     2161 accgcgcacg tcgccctcct cacccgcctg cgcgccgaga acccggcgct gcgtccgagc
     2221 agatatgcac gactgggtga gcacatcccc gactcctcgg tgatggactg gttcgaccag
     2281 aacggcgaga ccatggaggt cgagcagtgg aacgaccccc gcaaccgcac cctgcagtac
     2341 gtggcggcca gcgtccctga gaacggcgag tccaatcgca tcctgctgat cgtgcacggc
     2401 accgagtcgc cgatcgacgt gcgcctgccg gactcgatcg aggccgcgac ggcgttcgtc
     2461 gagctgtggt ccagcgccga cgacacgccg cagagcgagc agcgccggca tgcaccgggc
     2521 gaggtgctgc ccgttccggg cacctcgatg cgcctgttcc gggtggaatg acgccttttc
     2581 agaggccggt cgcgacccgc agccggaggg gatggcgcgg ggcccgctga gcggtacatt
     2641 cgtggtgtgg cagcacgaac cgctcaagaa cccgcggcgc tgagaagccc cgcccggatt
     2701 cctctgcggc ctgccgccga gaccgacgcc ccggaaggcg tgaaggcggg gaggatcccg
     2761 ctcttctccc ccaccccgca cgtgccccag gggtacctgc cgaaggcgtt cgcgggcgag
     2821 gtcgtgccct tcggcgtcgt ctccttccgc gagggtcacg atcgcatcgg cgtgcacgtg
     2881 cgcctgaccg acccctccgg cggcgagtcc ctgcaccggc tcaccgcgcg tcaggacggc
     2941 accgaccgct gggtcgccga catcgccctc gacgtcgagg gcccgtggcg gtaccgcttc
     3001 gaaggcttcg cggacgactt cgccacctgg gcgcacgcgg catccgtcaa ggcggaggcc
     3061 ggagtcgaca tcgcggtcat gagcgctctc ggcgcgacgc tgctgacctc cgccgcggcg
     3121 gagaaggatc gcccggccgc cgagcgcacc c
//
LOCUS       DATMSX010000377         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_53153,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000377 DATMSX010000000
VERSION     DATMSX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53153"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..552)
                     /locus_tag="VN041_11545"
     CDS             complement(88..552)
                     /locus_tag="VN041_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HWU29707.1"
                     /translation="MRRTSPGVLIVIGLLSAAAGYGFDHLLTITGNATFTPTIFLPVL
                     LVLLGATCLLLAWPVRRSVRSGIRIDPFRAVRASMLAQASSLLGAILAGFGAGLLAFL
                     STRPVPAPVGSTVAMVALVVGALLLIGAALLAEQFCTLPKDSDDREPDDPAP"
     gene            complement(549..1082)
                     /gene="folK"
                     /locus_tag="VN041_11550"
     CDS             complement(549..1082)
                     /gene="folK"
                     /locus_tag="VN041_11550"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWU29708.1"
                     /translation="MDVRLTHLPEPPDPRPGREPQVAVIALGSNEGDRGETLHAAAER
                     LRRLPLVDDVVMSDPIESVAVKLDGPDPDAPRYLNAVALVTTRLAPQVLLGMMHAVEE
                     EQGRVRRERWGDRTLDLDLIAYGDFRSSEEHLQVPHPRAAERLFVLEPWLSLDPDAVL
                     PGAGRVDELVRRLKDEP"
     gene            complement(1087..1458)
                     /gene="folB"
                     /locus_tag="VN041_11555"
     CDS             complement(1087..1458)
                     /gene="folB"
                     /locus_tag="VN041_11555"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWU29709.1"
                     /translation="MDFLDEILLTGLTVFGYHGVFDFERRDGQEFTIDLKLGLPLKDA
                     AASDDVADTVHYGELADKVAAIVAGEPVNLIETLAQRIADAVLEDERVKTVTVTVHKP
                     HAPIEQTFADVAVSIHRMQKA"
BASE COUNT          235 a          535 c          576 g          210 t
ORIGIN      
        1 ggcggcaccg gagcggcggg cggcaccggc gccgagggga tcagcggcga ctcgggtgcc
       61 cgcgtcggct gctcgaatcc atggggatca cggggcgggg tcgtcgggct ctcggtcatc
      121 agaatccttc ggcagagtgc agaactgttc ggcgaggagc gccgccccga tcagcagcag
      181 cgcgccgacg accagggcga ccatcgccac agtcgaccct accggcgcgg gcaccggccg
      241 tgtggagagg aaggcgagga ggccggcgcc gaagccggcg aggatcgctc ccagcagact
      301 cgacgcctgg gcgagcatgg aggcccgcac cgcgcggaag gggtcgatgc ggatgccact
      361 gcgcacgctg cgccggaccg gccaggccag cagcaggcag gtcgcaccga gcagcacgag
      421 cagcaccggc aggaagatcg tcggcgtgaa ggtcgcgttt ccggtgatgg tcagcaggtg
      481 atcgaagccg tagcccgcgg cggccgagag cagtccgatg acgatcagca cgccggggga
      541 tgtgcgtctc acggctcgtc cttcagccgg cgcaccagct cgtccacgcg tccggcgccg
      601 ggcagcacgg catccgggtc gaggctcagc cagggctcga gcacgaacag cctctccgcc
      661 gcgcgcgggt gcggcacctg caggtgctcc tcggagctgc ggaagtcgcc gtaggcgatc
      721 aggtccaggt cgagggtgcg gtcgccccag cgctcccggc gcacccgccc ctgctcctcc
      781 tcgaccgcgt gcatcatgcc cagcagcacc tgcggcgcca ggcgcgtggt cacgagggcc
      841 acagcgttga gatagcgcgg ggcgtccggg tcgggcccgt cgagcttgac ggcgacgctc
      901 tcgatcggat cggacatcac gacatcgtcg accagcggaa ggcgacgcag ccgctcggct
      961 gcggcgtgca gcgtctcccc ccggtcgccc tcgttggacc cgagggcgat gacggcgacc
     1021 tgcggctcgc gacccgggcg ggggtcaggc ggctcgggaa ggtgggtcag gcggacatcc
     1081 atgcggtcac gctttctgca tccggtggat cgagacggca acgtcggcga acgtctgctc
     1141 gatgggcgcg tgcggcttgt gcacggtgac ggtgacggtc ttgacgcgct cgtcctcgag
     1201 caccgcgtcc gcgatccgct gcgcgagggt ctcgatcagg ttcaccggct cgccggcgac
     1261 gatcgcggcg accttgtcgg ccagctcgcc gtagtgcacg gtgtcggcga cgtcgtcgga
     1321 tgccgcggca tccttcagcg gcaggccgag cttcaggtcg atggtgaact cctggccgtc
     1381 gcgacgttcg aagtcgaaca cgccgtggta accgaagacc gtcagcccgg tcagcaggat
     1441 ctcgtcgaga aagtccatct catgctccat cccaggcgcc ggcgatggcc agcgcgtccc
     1501 tcgtggctgc gacgtcgtgc acccgcaccg cccaggcgcc ggcccgcgcg gcgagt
//
LOCUS       DATMSX010000378         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_129166,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000378 DATMSX010000000
VERSION     DATMSX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129166"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VN041_11560"
     CDS             complement(<1..243)
                     /locus_tag="VN041_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29710.1"
                     /translation="MTPSSSPPADRTRAVGVRDVAALAGVSRQTVSRVLNEHPEVAEE
                     TRRRVLSAMAELGYRMNNAARALGTRRSRTLGVLASN"
     gene            complement(347..1030)
                     /locus_tag="VN041_11565"
     CDS             complement(347..1030)
                     /locus_tag="VN041_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29711.1"
                     /translation="MSVPELRSVVRLDRQPSIRDTVKRALRSAIISGDMQPGQVYSAP
                     SLGDQFGVSATPVREAMLDLVREGLVIAVPNRGFRVTEVSADDLREVTDLRLFLEPPA
                     IERATPLIPEEDFPALRAQADDIVAGAEEGDLVRYLNADSDFHLALLRYAGNARLIDL
                     ITSLRSQTRLFGLSALLQRGQLRDSASEHHAMIDAIEARDASLARRLVHKHIEHVLTD
                     WSGAPPGRE"
     gene            complement(1131..>1578)
                     /locus_tag="VN041_11570"
     CDS             complement(1131..>1578)
                     /locus_tag="VN041_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWU29712.1"
                     /translation="LLRAATWDGAQARIDVRIGDGPWQRYDGPLRVDVEGEVVVQARA
                     IAGGRTGTATRVLHIDTLPPVTQARVTDLGASVEILLVAEDEVSGVEQIHWQGEGTFW
                     ATFQEAFVRTLTDREQVIEFAASDRAGNEEPLRRLVLPARSIVERG"
BASE COUNT          217 a          579 c          543 g          239 t
ORIGIN      
        1 gttcgaggcc agcacgccca gggtgcgcga tcgccgggtg cccagggccc gcgcggcgtt
       61 gttcatccgg tagccgagtt cggccatcgc ggacagcacc cgccggcggg tctcctcggc
      121 gacctccgga tgctcgttca gcacgcgaga caccgtctgc cgggagaccc cggcgagcgc
      181 ggcgacatcg cgcacaccga cggcacgcgt gcggtctgcg ggcggggagc tcgacggggt
      241 cacgccgaag agtgtacgtc gccgagcgct tcgtcgtccg tcgggtgagg gccgtgcttc
      301 ggcatgacca cgtcccggac atcggcggcg ggcgcggtga ggcggctcac tcgcgccccg
      361 gtggcgcgcc cgaccagtcc gtgaggacgt gctcgatgtg cttgtgcacc aggcgccgcg
      421 ccagggaggc gtctcgggcc tcgatcgcgt cgatcatggc gtggtgctcc gatgccgagt
      481 ccctcagctg accgcgttgg agcagcgcgc tcagtccgaa cagccgggtc tgcgaccgca
      541 acgaggtgat caggtcgatg agtcgcgcgt tgcccgcgta gcgcagcaga gccaggtgga
      601 agtcgctgtc ggcgttgagg taacggacca ggtcgccctc ctccgcaccg gcgacgatgt
      661 cgtcggcctg cgcgcgcagc gcggggaagt cctcctccgg tatcagcggg gtggcacgct
      721 cgatcgccgg cggctcgagg aacaaccgca gatcggtcac ctcacgcagg tcgtcggctg
      781 acacctcggt gacccggaag ccccggttgg gcaccgcgat caccagcccc tcgcgcacga
      841 gatcgagcat cgcctcacgg accggggtcg ccgagacgcc gaactggtcg cccaggctcg
      901 gcgccgaata cacctggccc ggctgcatgt caccgctgat gatggcggat cgcagggcgc
      961 gcttgaccgt gtcccggatg ctgggctgcc ggtccagccg cacgacgcta cgcagctcgg
     1021 gaacgctcat ggcgatctca ttccttccgt cctgggcggc tctccgatcc gctgggttcg
     1081 atcctcgcac gccgtccacc cggatgtctg gcagctggtc ctgcgccggt tcagcccctc
     1141 tccacgatcg acctcgccgg aagcaccaga cgtcgcagcg gctcctcgtt tcccgcgcgg
     1201 tcggatgccg cgaactcgat gacctgctcg cggtcggtga gcgtgcgcac gaacgcctcc
     1261 tggaacgtcg cccagaaggt cccctcgccc tgccagtgga tctgctccac gccggacacc
     1321 tcgtcctcgg ccaccagcag tatctccacg ctcgcaccga ggtcggtgac ccgagcctgc
     1381 gtcaccggcg gcagggtgtc gatgtgcagc acgcgggtag ccgtgcccgt gcgtcctccg
     1441 gcgatcgccc gggcctgcac gacgacctcc ccttcgacat ccacgcggag gggcccgtcg
     1501 taccgctgcc acggcccgtc accgatgcgc acgtcgatcc gcgcctgcgc gccgtcccag
     1561 gtggcggcgc gcagcagg
//
LOCUS       DATMSX010000379         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176364,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000379 DATMSX010000000
VERSION     DATMSX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176364"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VN041_11575"
     CDS             <1..822
                     /locus_tag="VN041_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWU29713.1"
                     /translation="AARDRARDADVLLVVGSKLGEAELWVDRLDARGTVIRIDLLASQ
                     IDKNQKADVGIVGDAAASLSALLTALGDGPARTGARVAETLAAVRAEVHELSPVNAAL
                     AEAIASTLPADAIVATDSSQIAYWGLMNVLRVAKPNSMPYMATYATLGYALPAALGAR
                     LGDPGRPVFAVTGDGALMFSMQEMITVVEQRLDVTVIVVDNGGYAEIRQNEADAGIAP
                     IGVDLVQPDWAALGTAFGGTGRHVSSADELAAAVQQAQAAGGLQVIHLDQSTFAL"
     gene            835..1797
                     /gene="speB"
                     /locus_tag="VN041_11580"
     CDS             835..1797
                     /gene="speB"
                     /locus_tag="VN041_11580"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWU29714.1"
                     /translation="MTSTPIGPADASKTPRYAGLSTFARLPRIEDVGETDIAVVGIPF
                     DSGVSFRPGARFGPEHVRASSRLLRPYNPAQDFSGWEAVQMADAGDIAANPFNIHTAV
                     EQIAEAATELGERVDRILTIGGDHTIAYPLLKAISAKHGPVAVVHFDAHLDTWDTYFD
                     EPITHGTPFRRASEDGFIDRTASMHVGTRGPLYGKGDLEDDERLGFSIITSEYVEEHG
                     VPAAMERLRQRIGDRPLYVSVDIDVLDPAHAPGTGTPEAGGLTSRELLRIIRGLADLD
                     IVGADVVEVAPAYDHAQITGIAASHVAYEILTAMARAIVRGRGE"
     gene            1818..>2591
                     /locus_tag="VN041_11585"
     CDS             1818..>2591
                     /locus_tag="VN041_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU29715.1"
                     /translation="MDTARPLAVYTDVEDTDPAPGIALLEAHGFEVRVLGTRDPQLIV
                     DGARDAVALLPGYASVTREMIEAMPNLEIIALMSMGFDYVDLEAATENGVWVTNVPSA
                     ATEEVATHALAIMLHSLRQLRFYLGSANPDDWNERAPKAPPRLSERTLGVIGLGRIGR
                     EFARQAGPLFGRVLGYDPLMPDTAEVRADLDALGVQRGSLDDVRASSDVLSLHLPLTP
                     ETERMVDAEFLAGIPQGALLVNVSRGALIDDDALAASLDA"
BASE COUNT          393 a          962 c          879 g          357 t
ORIGIN      
        1 gccgcgcgcg accgcgcccg tgacgcggac gtgctgctcg tggtcggctc gaagctcggc
       61 gaggccgagc tctgggtcga ccggctcgac gcccgcggca ccgtgatccg catcgacctg
      121 ctcgcttccc agatcgacaa gaaccagaag gccgatgtgg gcatcgtcgg cgacgctgcg
      181 gcatccctct ccgcactcct gaccgcactg ggcgatggtc ccgcgcgcac gggcgcacgc
      241 gtcgccgaga ccctcgcggc tgtgcgcgcc gaggtgcacg agctgtcgcc ggtgaacgcg
      301 gctctcgccg aggccatcgc gtcgacgttg ccggccgatg cgatcgtcgc caccgactcg
      361 tcgcagatcg cgtactgggg tctgatgaac gtgctgcgcg tggcgaagcc gaactcgatg
      421 ccgtacatgg cgacctacgc caccctcggc tacgccctgc ccgcggctct cggcgcacgc
      481 ctaggagatc ccgggcgtcc ggtcttcgcc gtgaccggcg acggcgccct gatgttctcg
      541 atgcaggaga tgatcacggt cgtcgagcag cgcctcgacg tcaccgtgat cgtggtcgac
      601 aacggcggct acgccgagat ccgccagaac gaggcggacg ccggcatcgc accgatcggc
      661 gtggacctcg ttcagcccga ctgggctgcg ctcgggacgg cgttcggcgg caccggccgt
      721 cacgtctcct ccgccgacga gctcgccgcg gccgtgcagc aggcgcaggc cgcgggcggc
      781 ctgcaggtca tccacctcga ccagtccacg ttcgccctct gagacaggag catcatgacc
      841 agcacaccga tcggcccggc ggacgcctca aagaccccgc gctacgcggg cctgtccacc
      901 ttcgcccgtc tgccccgcat cgaggacgtc ggcgagaccg acatcgccgt cgtcggcatc
      961 ccgttcgaca gcggagtgag cttccgcccg ggcgcgcggt tcggccccga gcacgtgcgc
     1021 gcgtccagca ggctgctgcg cccgtacaac cccgcgcagg acttctccgg ctgggaggcc
     1081 gtgcagatgg cggatgccgg cgacatcgcc gcgaatccgt tcaacatcca caccgcggtc
     1141 gagcagatcg ccgaggccgc gacggagctc ggcgagcgcg tcgaccgcat cctcacgatc
     1201 ggcggcgacc acacgatcgc ctaccccctg ctgaaggcga tcagcgccaa gcacggcccg
     1261 gtcgccgtcg tgcacttcga cgcccacctc gacacctggg acacctactt cgacgagccg
     1321 atcacgcacg gcaccccgtt ccgccgcgcc tccgaggacg gcttcatcga ccggaccgca
     1381 tcgatgcatg tcggcacgcg cgggccgctc tacggcaagg gcgacctcga ggacgacgag
     1441 cgcctcggct tctcgatcat cacgagcgag tacgtcgagg agcacggtgt acctgccgcc
     1501 atggaacggc tgcggcagcg catcggcgac aggcccctgt acgtgtcggt ggacatcgac
     1561 gtgctcgacc ccgcgcacgc gccgggcacc ggcacccccg aggcgggcgg gctcaccagc
     1621 agggagctgc tccgcatcat ccggggcctg gcggatctgg acatcgtcgg cgccgacgtg
     1681 gtcgaggtcg ccccggccta tgaccacgct cagatcacgg gcatcgccgc cagccacgtg
     1741 gcatacgaga tcctcaccgc catggcacgc gcgatcgtgc gcggacgcgg cgagtgagcg
     1801 ccggaggagg agagcacgtg gacaccgctc gaccgctcgc cgtctacacc gacgtcgagg
     1861 acaccgatcc ggctcccggc atcgccctgc tggaggcgca cggcttcgag gtgcgcgtgc
     1921 tcggcacgcg cgacccgcag ctgatcgtcg acggtgctcg cgacgctgtg gccctgctgc
     1981 ctggctacgc gtccgtcacc cgcgagatga tcgaggcgat gccgaacctc gagatcatcg
     2041 cgctgatgtc gatgggcttc gactacgtcg acctcgaagc cgccaccgag aacggggtgt
     2101 gggtgacgaa cgtgcccagc gccgccaccg aggaggtcgc gacccacgca ctggcgatca
     2161 tgctgcacag tctgcgccag ctgcgcttct acctgggcag cgcgaacccc gacgactgga
     2221 acgagcgcgc gccgaaggcg cccccgcgcc tgagcgagag gacgctcggg gtgatcgggc
     2281 tgggcaggat cggcagggag ttcgccaggc aggcagggcc cctgttcggt cgcgtgctgg
     2341 ggtacgaccc tctcatgccc gacaccgcgg aggtgcgcgc cgacctcgac gccctgggcg
     2401 ttcagcgcgg gagcctcgac gacgtgcggg catcctccga cgtgctgtcg ctgcacctgc
     2461 cgctcacgcc cgagaccgag cgcatggtcg acgccgagtt cctcgccggg atcccgcagg
     2521 gggcgctgct ggtcaacgtg tcgcgcggcg cgctgatcga cgacgacgcg ctcgccgcgt
     2581 cgctcgacgc c
//
LOCUS       DATMSX010000380        10750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72236,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000380 DATMSX010000000
VERSION     DATMSX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72236"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VN041_11590"
     CDS             <1..381
                     /locus_tag="VN041_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HWU29716.1"
                     /translation="RAALLRMVGIAEGQAVPPALRPQLSALEDDQKKRATRSLRDGID
                     RVLTDLQSMYRDVVMLQFGRTGELINSELRTELEALAAAWPVERALIALDAISDTRES
                     LERNVSPLLATESLLVTISSGRTP"
     gene            378..1928
                     /locus_tag="VN041_11595"
     CDS             378..1928
                     /locus_tag="VN041_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29717.1"
                     /translation="MTHRARRAARRILTVLAGVAAASVVLSGCLYAQIPAGAPAPERT
                     ADTSGVSAELLPYYSQKLTWKSCGAGFDCTEVKAPRDWDKPADGDLELAIVRHRATGT
                     SQGSLLINPGGPGASGYDFVHDSLDYAVGADLIEDFDVIGFDPRGVGRSTAVRCLDAK
                     KMDDYLYGVVPGPRNTPEWDAALSARDKTFADACEANSDGLLPYITTVNSARDMDLIR
                     GVLGDKTLNYLGYSYGTFLGATYAKLYPERAQRLVLDGAIDPAVPGLEVSTIQAEGFE
                     SALRAYLEGCLAGRDCPFNGSVDEALTDFRALLSSVSRTPMKNGDGRMLTVDSMITGT
                     IAAMYSQESWSYLTKGITAALQGDPSVMMGLADSYNSRNADGTYADNTAEAFRAYNCM
                     DYPVEDDPAAEDAVVAQLEAKAPTFAPYWQAPDPCKVWPYPPTGVREAITAEGSGPIV
                     VVGTTNDPATPYAWAESLAKQLQNGVLVTRVGEGHTGYNKGNSCVDQAVERFLLDGKA
                     PKDGLRCG"
     gene            2008..2079
                     /locus_tag="VN041_11600"
     tRNA            2008..2079
                     /locus_tag="VN041_11600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2040..2042,aa:Thr,seq:cgt)
     gene            2172..2765
                     /locus_tag="VN041_11605"
     CDS             2172..2765
                     /locus_tag="VN041_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWU29718.1"
                     /translation="MSRALLIVDVQNDFTEGGALAVAGGDAVASAITAHLAAHAGEYA
                     VVIASRDWHDAEGDNGGHFSAEPDYVDSWPVHCVAGSDGAAYDPLLVTGAVTHHVLKG
                     QGVPAYSMFEGRTEDGETVGGILTAHGVTDADAVGIATDHCVRASVLDAIAHGVHVRV
                     LTDLIAGVDEQASRVALAELAHAGAELVDGSTGSEAR"
     gene            2762..3301
                     /locus_tag="VN041_11610"
     CDS             2762..3301
                     /locus_tag="VN041_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWU29719.1"
                     /translation="MSVRCALLLRAVNVGGRNRVPMAELRALLGERTDLEDIATYIAS
                     GNVMADVAGDIGEACAQVRRLIHEEFDADTPVIARTHEQLVAALEANPFPDAVADKML
                     HIMFLEGEPQPDAEEALRARLQPGERIALVGGDLWIDYGLSGAANTKLTRPVLDRALG
                     TAGTARNVLTLRKLIELTA"
     gene            3341..3994
                     /locus_tag="VN041_11615"
     CDS             3341..3994
                     /locus_tag="VN041_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HWU29720.1"
                     /translation="MVSPQHPATIAAAVDHELVIRKSRFIAHVAPVSSPEDADAVIAR
                     VKKQYWDARHNCSAQVTGIDGDRARSSDDGEPSGTAGIPMLEVLRRRELTDVVAVVTR
                     YFGGIKLGAGGLVRAYSSAVSEALDLASLVDRLMLAQLTIAVPHADAGRFDNLLRDWT
                     DRNGAVLGEPQYREQAVFEVWSPADGIAVLQAEIAAASGGSVRVSLTGTERVVDVPR"
     gene            4048..4827
                     /locus_tag="VN041_11620"
     CDS             4048..4827
                     /locus_tag="VN041_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU29721.1"
                     /translation="MDLGLRGRYALVTASSAGLGYACAAQLAKAGATVVLNGRDGAAL
                     SGAAERLRADTKADVRVVAGDVSNATVQEALVRAVPQVDILVNNNGGPPFRDFREVDR
                     DALLAGVTANMATPLELVQRVVDGMIERRFGRIVNITSFSVKMPIAGLDLSSGARAGL
                     TGFLAGVARDVAHANVTINFLLPGYFATDRLRALHVREAQERGVPVESVAVQGAEGIP
                     ARRFGHPDEFGAACAFLCSEQAGYITGQSLLIDGGAYPGIL"
     gene            complement(4809..5039)
                     /locus_tag="VN041_11625"
     CDS             complement(4809..5039)
                     /locus_tag="VN041_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29722.1"
                     /translation="MTASRTAPIRIPSPLPRHEHAWMTESAHRTSEGEIVYVRCTGCG
                     ARRVDLRGADGMPPAPWSRVSPAVPGTQRMPG"
     gene            5065..5583
                     /locus_tag="VN041_11630"
     CDS             5065..5583
                     /locus_tag="VN041_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HWU29723.1"
                     /translation="MHLNPYGEYAVLLAASLANEWPDHRDGIEQRTREFGMTMAFDVA
                     SDDHVRTRRVVDAWLAVVDAEDAAARAAALNRQMAAAAAYPQLTDHDGEGWHLHYRDA
                     GSSLPDVLEAVISVGTALHLTERGMHRLGRCAAAPCANVYVDITRNGRQRYCSVRCAN
                     RDAVRRHRARGI"
     gene            complement(5614..6441)
                     /locus_tag="VN041_11635"
     CDS             complement(5614..6441)
                     /locus_tag="VN041_11635"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWU29724.1"
                     /translation="MTALDDALAAIETWPVDNAAAAVVSVEGAVLAAQGDVSRPFRLA
                     SVTKPLTAYAVLIAVEEGVFSLDTPAGPEGSTVRHLLAHASGLDMTEDVVRAAPGTRR
                     IYSNRGFEVLADAVTEHSGIPFAEYLEEAVFQPLGMSSSQLQGSPAAAAVSTVADLCR
                     FAAELQRPALISTQTLADATAVAFPGLYGVLPGYGIQRPNDWGLGFELRDGKTPHWTS
                     AANSPATYGHFGQSGTFLWVDPDAGVACVALADRDFGPWAIEAWPPLSDAVLAALRG"
     gene            6561..7049
                     /locus_tag="VN041_11640"
     CDS             6561..7049
                     /locus_tag="VN041_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29725.1"
                     /translation="MSVSRTTATLAGLALIVLPFVALLVKFVVPGWMLVFMLLYSPVL
                     LIGWATQIVIAANGLLRARGVLRTGSAGLRPIIAAWLTSVAVVGVGFFLIDGGDDGNS
                     GSAFTELAGTSSTSAGSDLSMILCLACAVVWAGAWLWLLIEWIAQLVLAGNARRAQAM
                     PA"
     gene            complement(7051..7123)
                     /locus_tag="VN041_11645"
     tRNA            complement(7051..7123)
                     /locus_tag="VN041_11645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7088..7090),aa:Lys,seq:ttt)
     gene            complement(7312..7974)
                     /locus_tag="VN041_11650"
     CDS             complement(7312..7974)
                     /locus_tag="VN041_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HWU29726.1"
                     /translation="MLSTKKKITAALSLGFAGILVLAGCTMNGGSEATSEPKAPETSD
                     SASQAPETMDPAANLVGPGCADYAKQVPDGPGSVQGMSQDPVAVAASNNPLLTTLVSA
                     VSGKLNPDVNLVDTLNGDEFTVFAPVDSAFGKIDPATIEALKTDSKTLTSILTYHVVP
                     GQIEPSDIVGMHKTVQGADVDVTGSGDMLKVNDANVICGGVQTANATVYLIDSVLMPP
                     AQ"
     gene            8190..9899
                     /locus_tag="VN041_11655"
     CDS             8190..9899
                     /locus_tag="VN041_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HWU29727.1"
                     /translation="MDLIIIGLLGGLITGISPCILPVLPVIFLTGGAQSARFEGQVAP
                     ARRSRPYLVILGLVLSFSVVTLAGSLLLSLLHLPQDVIRWAGIVVLILIGVALIVPRL
                     EQLLERPFQWVPRREVQNRGSGFGVGLALGAVFVPCAGPVLAAIIVAGSTGRIGIGTV
                     LLTASFAIGVAVPLLVFALAGRGLVERIRAFRTRERGLRITAGVAMIALAVGLVFNVP
                     QQLQRLLPDYTAPLQQDLADKQADALDLGGLVTDENRELGNCTNGSATLQDCGTAPAI
                     RGIEQWLNTPDGKAIDLKQLRGKVVLVDFWAYSCINCQRSIPHVVAWDKAYRDAGLQV
                     IGVHSPEYAFEKDAGNVASGIRDFGIDYPVALDNDLATWTAYRNRYWPAHYLIDAKGV
                     VRQIAFGEGAYSATEKLIRQLLEQADPDVSLPAATEVADSTPEVGSTTPETFLGFSKD
                     RNYGGEGAYRSGEKDFAFPAKQPKDTFALSGRWDVQSQYAAPSEGSDGSSIRLTYRAD
                     EVRMVMGGSGRIVVTGPDGATERIDVDGSPRSYRVKQGGPAVGELTVEVPPGVQVYSF
                     TFG"
     gene            9934..10533
                     /gene="sigK"
                     /locus_tag="VN041_11660"
     CDS             9934..10533
                     /gene="sigK"
                     /locus_tag="VN041_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HWU29728.1"
                     /translation="MLVEMVIDGLEVPEDGSARDSAADLLVRVAAGDQRAFAELYDLL
                     SPRVFALILRVVVNRSQSEEVLQEVFLEIWQSAGRFAPNRGQGRSWVLTIAHRRAVDR
                     VRASQSSSDRDVRVGVRDLDAARDVVEEAVEAQIEGERVVAALSALPEAQQEALILAY
                     YGGYSQTEISALTGTPLGTIKTRMRDGLTRLRSELGVST"
     gene            10530..>10750
                     /locus_tag="VN041_11665"
     CDS             10530..>10750
                     /locus_tag="VN041_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29729.1"
                     /translation="MNEREFTELAAGHALHALSAVDERRFLEARAAHPEWREHIDEDE
                     QTAATLAAALPPVTPPPMLREELLARIATT"
BASE COUNT         1583 a         3705 c         3832 g         1630 t
ORIGIN      
        1 cgcgctgcgc tgctgcgcat ggtcggcatc gccgaggggc aggccgtgcc gcccgcgctg
       61 cggccgcagc tctcggccct cgaggacgat cagaagaagc gggccacccg cagcctgcgc
      121 gacgggatcg accgggtgct caccgacctg cagtcgatgt accgcgacgt cgtcatgctg
      181 cagttcggtc gtaccggcga gctgatcaac agcgagctgc gcaccgagct ggaagccctc
      241 gccgcggcct ggccggtcga gcgcgcgctc atcgccctcg acgccatcag cgacacccgt
      301 gagtccctgg agcgcaacgt ctcgccgctg ctcgcgaccg agagtctcct ggtgaccatc
      361 tccagcggaa ggaccccgtg acccatcgag cacggcgcgc tgcgcgccgc atcctcaccg
      421 tcctggccgg agtcgccgcg gcatccgtcg tgctctccgg atgcctgtac gcgcagatcc
      481 ccgcgggggc gcctgcgccg gagcgcacgg ccgacacgag cggcgtctcc gccgagctcc
      541 tgccgtacta ctcgcagaag ctcacctgga agtcgtgcgg cgccggcttc gactgcaccg
      601 aggtgaaggc gccccgcgac tgggacaagc ccgccgacgg cgacctcgag ctcgcgatcg
      661 tgcggcaccg cgcgaccggc acctcgcagg gctcgctgct gatcaacccc ggcggacccg
      721 gcgcgagcgg ctacgacttc gtccacgaca gcctggacta cgccgtcggc gccgatctga
      781 tcgaggactt cgacgtgatc gggttcgacc cccgcggcgt cggccgttcc accgccgtgc
      841 gctgtctgga cgcgaagaag atggacgact acctctacgg cgtcgtgccc ggtccgcgca
      901 acacgccgga gtgggatgcc gcgctctccg cgcgggacaa gaccttcgcc gacgcctgcg
      961 aggcgaacag tgacggcctg ctgccgtaca tcaccacggt gaactcggcg cgggacatgg
     1021 acctcatccg cggcgtcctg ggcgacaaga ccctgaacta cctcggctac tcctacggca
     1081 ccttcctcgg ggcgacctac gcgaagctgt accccgagcg ggcgcagcgg ctcgtgctgg
     1141 acggcgccat cgatcccgcc gtgcccgggc tcgaagtcag caccatccag gccgaaggct
     1201 tcgagtcggc gctgcgcgcc tacctggagg ggtgcctggc gggccgggac tgcccgttca
     1261 acggctcggt cgacgaggct ctcaccgact tccgcgcgct gctgtcgagc gtctcgcgca
     1321 cgccgatgaa gaacggcgac gggcggatgc tgacggtcga ctccatgatc accggcacga
     1381 tcgcggcgat gtacagccag gagagctggt cctacctgac caaggggatc accgcagctc
     1441 tgcagggcga cccgtccgtg atgatggggc tcgccgactc gtacaactcg cgcaatgccg
     1501 acggcaccta cgccgacaac accgccgagg cgttccgcgc atacaactgc atggactacc
     1561 cggtcgagga cgatccggcc gccgaggacg ccgtggtcgc gcagctggag gccaaggccc
     1621 ccacgttcgc cccgtactgg caggcgccgg acccctgcaa ggtgtggccg tacccgccga
     1681 cgggcgtgcg cgaggcgatc accgccgaag gttcgggacc gatcgtcgtg gtcggcacca
     1741 ccaacgaccc ggccaccccg tacgcgtggg cggaatcgct cgcgaagcag ctgcagaacg
     1801 gcgtcctcgt cacccgggtg ggcgaggggc acaccggcta caacaagggc aacagctgcg
     1861 tcgaccaggc cgtcgagcgc ttcctgctcg acggcaaggc tcccaaggac ggtctgcgct
     1921 gcggatgacc cgccgatgcg gcctcggttt cgcggaggcc gcatcctcgt agtaggatca
     1981 ttgcttgtgc cgcagtccgc tgcgcacgcc accttagctc agtggtagag catcccactc
     2041 gtaatgggaa ggccgtcagt tcaatcctga caggtggctc agatcggccc cggaactcca
     2101 gttccggggc ctttcccgtt ccgccgggct ccctctttcc gcgcccgccg tgcgccgcgt
     2161 acgctcgagg gatgagcaga gcactcctca tcgtcgacgt gcagaacgac ttcaccgagg
     2221 gcggcgcgct cgccgtcgca ggcggggatg ccgtggcatc cgccatcacc gcgcacctcg
     2281 ccgcgcacgc gggggagtac gccgtggtca tcgcctcgcg ggactggcac gacgccgagg
     2341 gcgacaacgg cgggcacttc tccgctgagc ccgactatgt ggactcctgg cccgtgcact
     2401 gcgtcgccgg ctccgacggc gccgcctacg atccgctgct ggtgacgggc gccgtgaccc
     2461 atcacgtcct caaaggccag ggcgtcccgg cgtactcgat gttcgagggc aggaccgagg
     2521 acggggagac ggtcggcggc atcctgaccg cgcacggcgt gacggatgcg gatgcggtgg
     2581 gcatcgcgac ggatcactgc gtgcgtgcat cggtgctgga cgccatcgcg cacggcgtgc
     2641 acgtgcgagt gctcaccgac ctcatcgcgg gtgtcgacga gcaggccagc cgggtcgcgc
     2701 tggccgagct cgctcacgcg ggcgcggagc tcgtggacgg ctcgaccgga tccgaagccc
     2761 ggtgagcgtc cgctgcgccc tgctgctgcg cgccgtcaac gttggcggca ggaaccgcgt
     2821 gcccatggcg gaactgcgcg ctctgctggg tgagcgcacc gacctggagg acatcgccac
     2881 gtacatcgcc agcggcaacg tcatggccga cgtcgccggc gacatcggcg aggcgtgcgc
     2941 tcaggtgcgc aggctgatcc acgaggagtt cgacgccgac acccccgtga tcgcgcgcac
     3001 tcacgagcag ctcgtggcgg cgctggaggc gaatccgttc ccggacgccg tggccgacaa
     3061 gatgctgcac atcatgttcc tggagggcga gccgcagccg gatgccgagg aggcgctgcg
     3121 cgcacgtctg cagccggggg agcgcatcgc gctcgtcggc ggtgacctgt ggatcgacta
     3181 cggcctgagc ggggcggcga acacgaagct cacgcggccg gttctcgacc gcgcgctcgg
     3241 cactgcaggc actgcccgca acgtcctcac gctgcgcaag ctcatcgagc tcacggcctg
     3301 agcgtgcgtg aaggtcggct ctgccgtcgc gtagcctgag gtggtgtcac cgcagcatcc
     3361 cgccacgatc gccgcggccg tggaccacga gctggtcatc aggaagtccc ggttcatcgc
     3421 gcacgtggcg cccgtgtcct cgcctgagga cgcggatgcg gtcattgcgc gggtcaagaa
     3481 gcagtactgg gacgcccggc acaactgcag cgcgcaggtc acggggatcg atggagaccg
     3541 ggcgcgctcc tcggacgacg gcgagccgtc cggaacggcg ggcatcccga tgctcgaagt
     3601 gcttcgccgc cgggagctga ccgatgtcgt cgccgtcgtc acccgctact tcggcggcat
     3661 caagctgggt gccggcggcc tggtgcgtgc gtattcctcg gcggtctcgg aggcgctgga
     3721 tctggcctcc ctcgtcgatc ggctgatgct cgcgcagctg acgatcgcgg tgccgcacgc
     3781 ggatgcgggg cgcttcgaca acctgctgcg cgactggacc gaccggaacg gcgccgtgct
     3841 cggcgagccg cagtaccgcg agcaggctgt cttcgaggtg tggagccccg cggacggaat
     3901 cgcggtgctg caggccgaga tcgcggccgc ctcgggcggg tcggtgcggg tatccctcac
     3961 cgggaccgag cgcgtggtcg acgtaccgcg ctgagccccg cggggcacgt gccgggcgat
     4021 cgcggcggac cgggggagaa tcgagccatg gatctcggac ttcgcgggcg ctacgccctt
     4081 gtcaccgcgt caagcgccgg actcggctac gcgtgcgccg cacagctggc gaaggccggc
     4141 gctacggtcg ttctcaacgg cagggacggc gcagcgctga gcggcgcagc ggagcgccta
     4201 cgtgcggaca cgaaggcgga cgtgcgcgtc gtggcgggcg atgtgtcgaa cgccacggtg
     4261 caggaggcgc tcgtccgtgc cgtcccgcag gtcgacatcc tcgtcaacaa caacggcggg
     4321 ccgccgttcc gcgacttccg cgaggtggat cgggatgccc tgctcgccgg cgtcacggcg
     4381 aacatggcga cgcctctcga gctcgtgcag cgtgtggtcg acggcatgat cgagcgtcgg
     4441 ttcgggcgca tcgtgaacat cacgtcgttc tccgtgaaga tgcccatcgc gggactcgac
     4501 ctctccagcg gcgcacgtgc ggggctgacc ggcttcctcg cgggggtggc cagggatgtc
     4561 gcgcacgcga acgtgacgat caacttcctg ctgcccgggt acttcgccac cgatcggctg
     4621 cgcgcgctgc acgtgcggga agcgcaggag cggggcgtgc ctgtcgagtc ggtcgccgtg
     4681 cagggcgccg aagggatccc tgcccgccga ttcgggcacc ccgacgagtt cggcgccgcg
     4741 tgcgcgttcc tgtgctcgga gcaggccggc tacatcaccg gccagagtct gctcatcgac
     4801 ggcggggcct acccgggcat cctctgagtg ccgggcacgg cggggctcac ccggctccac
     4861 ggagcgggcg gcatcccgtc tgcgccgcgc aggtcgacgc gacgcgcccc gcatcccgta
     4921 cagcgcacgt agacgatctc gccttcggag gtgcggtgcg cggactcggt catccaggcg
     4981 tgctcgtgcc gggggagcgg gctcgggatg cggatcggtg cggtacggga tgcggtcatg
     5041 tcaccacgat gatgtaatgg ccttatgcat ctcaaccctt acggcgagta tgcggtcctg
     5101 ctcgccgcat ccctggccaa cgagtggccc gaccaccgtg acgggatcga gcagcgcacg
     5161 cgcgaattcg ggatgacgat ggcattcgac gtcgcctccg acgaccacgt ccgcacccgg
     5221 agggtggtgg atgcctggct cgccgtggtg gatgccgagg acgccgccgc ccgtgctgcg
     5281 gccctgaacc ggcagatggc cgcggcggcc gcatatccac agctcaccga ccacgacgga
     5341 gaagggtggc atctgcatta ccgcgatgcc ggaagctcgc tgcctgacgt gctcgaggcc
     5401 gtgatcagcg tgggcaccgc gctgcatctg accgagcgcg gcatgcacag gctcgggcgc
     5461 tgcgccgccg cgccgtgcgc gaacgtctac gtcgacatca cccgcaacgg acggcagcgt
     5521 tactgctccg tgcgctgcgc gaaccgcgac gcggtccggc gccatcgcgc gcgcggtatc
     5581 tgagctcggg tccccgagcc tgtcgacggg tggtcagcct cgcagcgcgg cgagcacggc
     5641 atccgacagc ggcggccatg cctcgatcgc ccacgggccg aagtcccggt cggcgagagc
     5701 gacgcaggcg acgccggcat ccggatccac ccacaggaac gttccgctct ggccgaagtg
     5761 cccgtaggtc gcgggggagt tcgccgccga ggtccagtgc ggcgtcttgc cgtcgcgcag
     5821 ctcgaacccc aggccccagt cgttggggcg ctggatcccg tagcccggca gcaccccgta
     5881 gagcccgggg aaggccactg cggtcgcatc cgcgagcgtc tgcgtcgaga tgagggccgg
     5941 acgctgcagc tccgcagcga agcggcacag gtcggcgacc gtggagacgg cggcggccgc
     6001 gggcgagccc tgcagctgcg aggacgacat gccgagaggc tggaacaccg cctcttccag
     6061 gtactcggcg aacgggatgc cggagtgctc cgtcaccgcg tcggccagca cctcgaaccc
     6121 ccggttcgag tagatccggc gcgtgccggg cgcggcgcgc accacgtcct cggtcatgtc
     6181 caggccggag gcgtgggcga ggaggtggcg aaccgtggat ccctccgggc ccgccggtgt
     6241 gtcgagggag aacacgccct cttcgacggc gatgagcacc gcgtacgcgg tgagcggctt
     6301 ggtgaccgac gccagccgga acggccggga gacgtcaccc tgcgcggcga ggacggcgcc
     6361 ctcgacggag acgacggcgg ccgcagcgtt atccaccggc caggtctcga tggcggcgag
     6421 tgcgtcgtcc agggcggtca tgggtctcct ccggctcggg tcggctcgtc gtgcgggagc
     6481 gggctcccgg cgccctggaa tcctcccata tcgcgccggc gtccgcggtc gcatggcggc
     6541 cgtctggcat gatcggggcc atgagcgtct cgcgcacgac cgccaccctc gcagggctgg
     6601 cgctgatcgt cctccccttc gtcgccctgc tggtgaagtt cgtcgtcccg ggctggatgc
     6661 tggtcttcat gctcctgtac agtccggtgc tgctgatcgg ctgggccacg cagatcgtca
     6721 tcgcggcgaa cgggctgctg cgggcgcgcg gagtgctgcg gacgggctcc gcaggcctgc
     6781 ggccgatcat cgccgcgtgg ctgacgtccg tcgccgtggt cggagtggga ttcttcctga
     6841 tcgacggcgg cgacgacggg aactcgggtt ccgcattcac cgagctggcc ggcacgagct
     6901 ccacgtcggc gggttcggac ctatcgatga tcctgtgtct cgcctgcgcg gtggtgtggg
     6961 cgggggcatg gctgtggctg ctgatcgaat ggatcgcgca gctggtgctc gcgggcaacg
     7021 cccgtcgagc acaggcgatg cctgcgtagg tgggccccgt ggggctcgaa cccacgaccc
     7081 gcggattaaa agtccgatgc tctgccaact gagctagagg cccctcgtca gggcggacct
     7141 gaccgatcca tcctatccgg cggagcggcc gcatcgtcga cccgtggacg cacgagcgcg
     7201 ctcgccggtc gaccggcgag cgcgctctcc tcggagtgtc ggtctcctcc ctgatccggc
     7261 actccggcag gtgatgccga ggcgtcccca cgcgccggca tcacctgacg gtcactgagc
     7321 cggcggcatc agcacggagt cgatcaggta cacggtcgcg ttcgcggtct gcacgccacc
     7381 gcagatcacg ttggcgtcgt tgaccttgag catgtcgccg gatccggtca cgtcgacatc
     7441 agcgccctgg acggtcttgt gcatgcccac gatgtcggag ggctcgatct gtcccgggac
     7501 gacgtggtag gtcaggatcg aggtgagcgt cttcgagtcg gtcttgagcg cctcgatggt
     7561 ggccgggtcg atcttgccga acgcggagtc caccggtgcg aagaccgtga actcatcgcc
     7621 gttgagcgtg tcgacgaggt tcacatcggg gttcagcttc ccgctcacgg cggagaccag
     7681 cgtcgtcagg agcgggttgt tcgacgcggc gacggcgacc gggtcctggg acatgccctg
     7741 cacggagccg ggaccgtccg gcacctgctt ggcgtagtcc gcacagccgg gaccgaccag
     7801 gttcgccgcc gggtccatcg tctcgggggc ctgggatgcg gagtcggacg tctccggtgc
     7861 cttcggctcc gaggtcgcct ccgatccgcc gttcatcgtg caacccgcca gcacgaggat
     7921 gccggcgaaa ccgagtgaga gagctgcggt gatcttcttc ttggtgctga gcatgtcaac
     7981 ctccggttgc tagggccgcg gacctcccgc ggcgtcgacg gttgttcggc gccctcgcac
     8041 gatcggatgg gatcttctcg gacgtctttc tccggcagca tccgtggctc cctcagggcg
     8101 tgcccgcgac gtgtccgcga cgcgccagcg gcgtctcagg caatccgaat ccgggccggg
     8161 gccgaacagc actcgacgga aggcggatca tggatctgat catcatcggg ctgctcggcg
     8221 gcctcatcac ggggatctcc ccgtgcatcc tgccggtgct cccggtcatc ttcctcaccg
     8281 gaggcgcgca gtcggcgaga ttcgagggtc aggtggcgcc cgcgcggcgg agccgcccct
     8341 acctggtgat cctgggcctc gtgctgagct tcagcgtcgt gaccctggcc ggatcgctgc
     8401 tgctctccct gctccacctg ccgcaggacg tcatccgatg ggcggggatc gtcgtcctga
     8461 tcctcatcgg cgtcgcgctc atcgtgcccc ggctcgagca gctgctggag cggcccttcc
     8521 agtgggtgcc ccgccgcgag gtgcagaacc gggggagcgg attcggcgtc ggactcgccc
     8581 tcggcgcggt gttcgtgccc tgcgcgggac ccgtcctcgc cgccatcatc gtcgcgggat
     8641 cgaccgggcg catcggcatc ggcacggtgc tgctcaccgc ctcattcgcg atcggcgtgg
     8701 ccgttccgct gctggtcttc gccctcgccg ggcgcgggct ggtcgagcgg atccgcgcgt
     8761 tccgcacgcg ggagcgcggc ctgcgcatca ccgccggtgt cgcgatgatc gccctggcgg
     8821 tgggactcgt cttcaacgtc ccgcagcagc tgcagcgcct gctcccggac tacacggcgc
     8881 cgctgcagca ggacctcgcc gacaagcagg cggatgccct cgacctgggt ggcctggtca
     8941 ccgacgagaa ccgggagctc ggcaactgca cgaacggctc ggccacgctg caggactgcg
     9001 gcacggcgcc ggcgatccgc ggcatcgagc agtggctgaa cacgccggac gggaaggcca
     9061 tcgacctgaa gcagctgcgc ggcaaggtgg tgctggtcga cttctgggcg tattcgtgca
     9121 tcaactgcca gcgctccatt ccgcacgtcg tcgcctggga caaggcgtac cgcgacgcgg
     9181 gcctgcaggt gatcggcgtg cattcgccgg agtacgcctt cgagaaggac gcgggcaacg
     9241 tcgcctccgg catccgcgac ttcggaatcg actatccggt ggcgctcgac aacgacctgg
     9301 ccacctggac cgcgtaccgc aaccgctact ggcccgcgca ctacctcatc gacgcgaagg
     9361 gcgtcgtgcg gcagatcgcc ttcggggagg gggcgtactc cgccaccgag aagctgatcc
     9421 ggcagctgct ggagcaggcc gatcccgatg tctcgctgcc cgccgccacg gaggtcgccg
     9481 acagcacgcc cgaagtgggc tcgaccaccc cggagacgtt cctgggattc tcgaaggacc
     9541 gcaactacgg cggtgagggt gcgtaccgat ccggtgagaa ggacttcgcg ttcccggcga
     9601 agcagccgaa ggacaccttc gcgctcagcg gccggtggga cgtccagagc cagtacgccg
     9661 caccgtccga gggctccgac ggcagcagca tccgtctgac ctaccgtgcc gacgaggtgc
     9721 ggatggtgat ggggggatcc ggccggatcg tggtgacagg cccggacggc gccaccgagc
     9781 gcatcgacgt ggacggcagc ccgcgttcgt atcgggtgaa gcagggcggg ccggcggtcg
     9841 gtgagctcac cgtcgaggtg ccgccgggcg tgcaggtcta ctcattcacc ttcgggtgag
     9901 agtggcgcgg gccgggagag gaggggatgc ggcatgctgg tggagatggt gatcgacggc
     9961 ctggaagtac cggaggacgg ttcggcgcga gacagcgcgg ccgacctcct cgtgcgcgtg
    10021 gccgcaggcg atcagagggc cttcgccgaa ctgtacgacc ttctctcgcc gcgcgtgttc
    10081 gccctgatcc tgcgggtggt ggtgaaccgc tcgcagagcg aggaggtcct ccaggaggtc
    10141 ttcttggaga tctggcaatc cgccggccgc ttcgctccga acagagggca gggcaggtcc
    10201 tgggtgctca ccatcgccca ccggcgggcc gtcgacaggg tgcgggcgtc gcagtcgagc
    10261 agcgatcgtg acgtgcgcgt cggtgtgcgt gacctcgatg cggcccggga tgtcgtcgaa
    10321 gaggcagtcg aggcgcagat cgaaggcgag cgggtcgtcg cggcgctctc ggcgctgccg
    10381 gaggcgcagc aggaagcact gatcctggcc tattacgggg gttacagtca gaccgagatc
    10441 tcggctctga ccggcactcc gctgggaacg atcaagacga ggatgagaga cgggctgacg
    10501 cggctgcgca gcgaactggg ggtgtcgaca tgaacgagcg ggaattcacc gagctcgccg
    10561 ccggacacgc tctgcacgcc ctctcggcag tggacgagcg gcgtttcctc gaggcgcggg
    10621 cagcgcatcc ggagtggcgc gagcacatcg acgaggacga gcagaccgcg gcgaccctcg
    10681 ccgccgctct cccgccggtg acgccgcccc cgatgctccg ggaggagctg ctcgcgcgca
    10741 tcgcgacgac
//
LOCUS       DATMSX010000381         5102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_44142,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000381 DATMSX010000000
VERSION     DATMSX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44142"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..515)
                     /locus_tag="VN041_11670"
     CDS             complement(45..515)
                     /locus_tag="VN041_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6114 domain-containing protein"
                     /protein_id="HWU29730.1"
                     /translation="MLLSDELAESSETAMTDAQHTPAESGFRHWRRQRPLIGGVLLVV
                     SGIEMFFSGQLDFGELHIQLGIEGLQATIIPILLVLLGVLAVLMPAHHVFYGVIALAV
                     ALYSLVGVNLGGFVVGMLLGCIGGILVVAWMPGRLTRRERRAARRDGQVEEQDA"
     gene            complement(506..1123)
                     /locus_tag="VN041_11675"
     CDS             complement(506..1123)
                     /locus_tag="VN041_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6230 family protein"
                     /protein_id="HWU29731.1"
                     /translation="MKFRSPKKLISKATASHAGRITLAAVPVGIVAAVLMGGVAQGQV
                     PVSFAVSGSQFQISASLLEGTGFSQYAGVTKDTEGGEHSVAIANIKSATLADLCQSVV
                     SETPLGKVGVLIKAGGGGNPASASDLQIGMTGLKGDASFGKIRIGVDASTVNTESKGT
                     AGDFAQDADSVTIKNLQQTAWSTQASVFTLTGMTLELTDGSQGCF"
     gene            1217..1816
                     /locus_tag="VN041_11680"
     CDS             1217..1816
                     /locus_tag="VN041_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29732.1"
                     /translation="MSAERRLRLTPDERREQLIAIGVNFLAEHTLDELTMEALAQRAG
                     VSRPLLFHYFDTRQGMHLAVVATARDSLLLATEPRPELPPRERIEDTLLRIAEFVQQH
                     QGTFHSLVRGIASGDSAVRRVVDESRERNAERLVEAFTELGAPDSPALRIALRAWVSF
                     TEETLISLLVERDASPADVVRFLLGTIDGVVAAAGELRI"
     gene            complement(1823..2743)
                     /locus_tag="VN041_11685"
     CDS             complement(1823..2743)
                     /locus_tag="VN041_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWU29733.1"
                     /translation="MQSRTLPVPDGLEGSRVDAALAKMLGFSRTFAAEVAEAGGVTLD
                     GVQLGKSDRLRAGGWLEVSWVPKEEPRIVPIAVPELGIVHDDDDIVVVDKPTGVAAHP
                     SLGWEGPTVVGALAAAGFRIATSGAPERQGVVHRLDVGTSGLMVVAKTEGAYKALKRA
                     FKERTVEKIYHAVVQGHPDPLVGTIDAPIGRHPNHHWKFAVVPDGKPSVTHYETLEAF
                     PGASLLEIHLETGRTHQIRVHMAAHRHPCVGDPLYGADPTLSARLGLTRQWLHAHQLA
                     FAHPATGEWVQFESPYPEDFQHALDVLRGE"
     gene            complement(2746..3276)
                     /gene="lspA"
                     /locus_tag="VN041_11690"
     CDS             complement(2746..3276)
                     /gene="lspA"
                     /locus_tag="VN041_11690"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWU29734.1"
                     /translation="MVAILAALVLAADQLVKYLTIQHLPSEKAVPVLGEFLQLFYIRN
                     SGAAFSIGASMTWVFTIALAVVAVVIVWKTLGVRSRLWAVVLGCLLGGVLGNLSDRLF
                     REPGFAVGHVVDMISMPWMLPAIFNVADVFIVTGMISVALLVVVGLRLDGTRERDHAK
                     GDDAPTADAAAAANEA"
     gene            complement(3326..3901)
                     /locus_tag="VN041_11695"
     CDS             complement(3326..3901)
                     /locus_tag="VN041_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWU29735.1"
                     /translation="MALTPDDVVHKEFQHVRFKDGFDPEEVDDYLDEIVVEWRKTLEE
                     NNELKAKLAAFESGQNTGPVAAAPVATAPTAPAQASGASATGTSAGIIELAQRLHDEH
                     VAEGESKRRQLIADAEAEVARIRTEAEAKQREESARLERERNTLEGRITELREFERDY
                     RGKLRAMIEGQLRDLDQKSTSDSTPVSAIGL"
     gene            complement(4050..4343)
                     /locus_tag="VN041_11700"
     CDS             complement(4050..4343)
                     /locus_tag="VN041_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWU29736.1"
                     /translation="MTPLSIIGGIAELVLTLYMIVLLARLVLDYIPMFNRGWRPKGVG
                     LVLAEVVFTLTDPPIRFFRRVIPPLRIGTIALDFGWTLTMILVIILLTVARAF"
     gene            complement(4349..4816)
                     /gene="sepF"
                     /locus_tag="VN041_11705"
     CDS             complement(4349..4816)
                     /gene="sepF"
                     /locus_tag="VN041_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HWU29737.1"
                     /translation="MGNPLKKTMVYLGLADEEEVYEEEATQPVARAPREREREREEHA
                     PAPVTPLRRPTAVHQPAAGAVNEILTVHPKQYRDAQVIAENFREGVPVIINLSQMSDA
                     DARRLIDFASGLSLGLYGRIERVTSKVFLLSPENIAVSGQGGLAQAESASFDS"
     gene            complement(4861..>5102)
                     /locus_tag="VN041_11710"
     CDS             complement(4861..>5102)
                     /locus_tag="VN041_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWU29738.1"
                     /translation="VLGLESLRLRGVMAVAPLDEDPASAFARLRAVADRIRVIAPEAT
                     WMSAGMTGDFVQAIDAGATHLRIGSAITGPRPDRG"
BASE COUNT          804 a         1742 c         1762 g          794 t
ORIGIN      
        1 gcacagcgcg gacgcgacga cgacggcgcg gacggggcga cggatcatgc gtcctgctcc
       61 tcaacctgcc cgtcccggcg cgccgcgcgc ctctcgcggc gcgtcagcct ccctggcatc
      121 caggcgacga cgaggatgcc gccgatgcag cccagcagca tccctacgac gaatccgccg
      181 aggttcacac ccacgagcga gtacagcgcg acggccagcg cgatgacgcc gtagaagacg
      241 tgatgcgcgg gcatcagcac cgccagcacg ccgagcagca ccagcaggat ggggatgatc
      301 gtggcctgca gaccctcgat gcccagctgg atgtgcagct cgccgaagtc cagctgcccg
      361 gagaagaaca tctcgatgcc ggacacgacg agcagcaccc cgccgatcag gggacgctgg
      421 cgccgccagt gacggaatcc cgattcggcg ggcgtgtgct gggcatccgt catggccgtc
      481 tccgaggact cagcgagctc gtcgctcaga agcatccctg cgatccgtcc gtgagctcca
      541 gcgtcatgcc cgtgagggtg aagacggacg cctgggtgct ccatgcggtc tgctgcaggt
      601 tcttgatggt gaccgagtcg gcgtcctgag cgaagtcgcc cgcggtgccc ttcgactcgg
      661 tgttcacggt cgacgcgtcc acgccgattc ggatcttgcc gaaggacgcg tcgcccttca
      721 gaccggtcat gccgatctgc aggtcggagg cgctggcggg gttgccgccg ccgccggcct
      781 tgatgagcac gcccaccttg ccgagcggcg tctcggagac caccgactgg cagaggtcgg
      841 cgagcgtcgc actcttgatg ttcgcgatcg cgaccgagtg ctcgccgccc tcggtgtcct
      901 tcgtgacgcc cgcgtactgg gagaacccgg tgccctccag aaggctcgcg ctgatctgga
      961 actggctgcc cgacacggcg aacgacacgg gtacctggcc ctgtgcgaca ccgcccatga
     1021 gcacggcggc gacgatgccg accggcacgg cggccagagt gatgcggcct gcgtgcgagg
     1081 cagtggcctt cgagatcagc ttcttcgggc tgcggaactt catcgatcct cctggtggtc
     1141 gcgtcgacat cggcgcagga gccagaatac cgctcattga cttctagtca ataccgcgcg
     1201 ccgataggat cgggacatgt ccgccgagcg tcgcctccgt ctcacgcccg acgaacgacg
     1261 ggagcagctg atcgccatcg gcgtcaactt cctcgccgag cacacgctcg acgagctgac
     1321 gatggaggcg ctcgcgcagc gcgccggcgt ctcccgccct ctgctgttcc actacttcga
     1381 cacccgccag ggcatgcacc tcgccgtggt cgcgaccgcc agggacagcc tgctgctggc
     1441 caccgagccg cgccccgagc tgccgccgcg cgagcgcatc gaggacaccc tgctgcgcat
     1501 cgccgagttc gtgcagcagc atcagggcac gttccactca ctggtgcgcg gcatcgccag
     1561 cggggactcg gccgtgcgcc gggtggtgga cgaatcccgc gaacgcaacg ccgaacgcct
     1621 ggtcgaggcg ttcacggagc tcggagcacc ggactccccc gcactgagga tcgccctgcg
     1681 ggcgtgggtg tcgttcacgg aggagacgct gatcagcctg ctcgtggagc gagacgcctc
     1741 ccccgcggac gtggtgcgct tcctgctggg caccatcgac ggtgtggtcg ccgccgccgg
     1801 cgagctcagg atctgaggcc gactactcgc cgcgcagcac gtcgagtgcg tgctggaagt
     1861 cctccgggta cggcgactcg aactgcaccc actcccccgt cgccgggtgg gcgaacgcga
     1921 gctgatgcgc gtgcagccac tgccgggtca ggccgagccg cgcggacagc gtgggatcgg
     1981 cgccgtacag cgggtcgccc acgcacggat gccggtgcgc agccatgtgc acgcggatct
     2041 ggtgcgtgcg gccggtctcc aggtggatct ccagcagcga agcgccgggg aaggcctcga
     2101 gtgtctcgta gtgggtcacc gagggcttgc cgtccggaac cacggcgaac ttccagtggt
     2161 ggttcggatg ccgtccgatc ggcgcgtcga tcgtgccgac gagcggatcg ggatgcccct
     2221 gcaccacggc gtggtagatc ttctcgaccg tgcgctcctt gaacgcgcgc ttgagcgcct
     2281 tgtaggcgcc ttcggtcttg gcgacgacca tcaggccgct ggttcccacg tcgaggcggt
     2341 ggacgacgcc ctgccgctcc ggcgcaccgc tggtcgcgat acggaacccc gcggcggcga
     2401 gagccccgac gacggtgggc ccctcccagc cgagcgaggg atgcgcggcc actcccgtgg
     2461 gcttgtcgac caccacgatg tcgtcatcgt cgtggacgat gcccagttcc ggcaccgcga
     2521 tcggcacgat ccgcggctcc tccttgggca cccagctcac ctcgagccag cctccggcac
     2581 ggagccgatc ggacttgccc agctgcacgc cgtcgagggt cacgcctccg gcctcggcga
     2641 cctcggcggc gaaggttcgc gagaatccca gcatcttcgc cagcgcggcg tcgacgcggg
     2701 acccctccaa cccgtccggg acgggaaggg tgcgactctg catgctcacg cctcgttcgc
     2761 ggcggccgca gcatccgccg tcggcgcgtc gtcgcccttg gcgtgatcgc gctcgcgcgt
     2821 gccgtccagg cggagaccga cgaccaccag cagcgccacc gagatcatcc cggtcacgat
     2881 gaacacgtcc gcgacgttga agatcgcggg cagcatccac ggcatcgaga tcatgtcgac
     2941 cacgtggccc acggcgaagc cgggctcgcg gaagagacgg tcggacagat tgcccagcac
     3001 gccgccgagc aggcatccga gcaccacggc ccacagccgg gagcgcacgc ccagggtctt
     3061 ccagacgatc accacggcga cgacggccag cgcgatcgtg aagacccagg tcatgcttgc
     3121 gccgatcgag aaggcggcgc cggagttgcg gatgtagaac agctgcagga attcccccag
     3181 caccggcacg gccttctccg agggcaggtg ctggatggtg aggtacttca caagctgatc
     3241 ggcggccagc acgagcgccg cgagaatcgc gacgatcgca ccggccgccg accgacgaag
     3301 gggacgacgt cctgtcaaga tcgacctaca ggccgatggc ggagacaggg gtcgagtcag
     3361 acgtcgactt ctggtccagg tcgcggagct gaccctcgat catggcgcgg agcttgccgc
     3421 ggtagtcgcg ctcgaactcg cgcagctcgg tgatgcgtcc ctcgagcgtg ttgcgctcgc
     3481 gctccaggcg cgcggactcc tcgcgctgct tggcctcggc ctcggtgcgg atgcgggcga
     3541 cctcggcctc ggcgtcggcg atcagctggc ggcgcttgga ctcgccctct gcgacgtgct
     3601 cgtcgtgcag gcgctgcgcc agctcgatga tgcctgcgga ggttccggtg gcggatgcgc
     3661 cgctggcctg agccggagcg gtcggggctg tggcgacggg ggctgcagcg accggaccgg
     3721 tgttctggcc ggactcgaag gcggcgagct tggccttcag ctcgttgttc tcctcgaggg
     3781 tcttgcgcca ctcaacgacg atctcgtcga ggtagtcgtc gacctcttcg gggtcgaagc
     3841 cgtccttgaa gcggacgtgc tggaactcct tgtggacgac gtcatccggg gtaagtgcca
     3901 tgggtggctc ctctttcgat gagttctttt cggccagtcg ttgaagacgg cgcggtgttc
     3961 ttcagacgcg tacccggggc gcgcgtctgg gagccaagca tagcccccca gcctgtgtgc
     4021 cgcgatgagc ggacgactcg gaggtcacct tagaaggcac gcgcgacggt cagcaggatg
     4081 atcaccagga tcatcgtcag ggtccagccg aagtccagtg cgatcgtgcc gatccgcagc
     4141 ggcgggatga ccctgcggaa gaaccggatg ggcgggtccg tcagagtgaa gacgacctcc
     4201 gccaggacca gaccgacgcc tttcggacgc cagccgcgat tgaacatcgg gatgtagtcc
     4261 agcaccagcc gggccagcag cacgatcatg tacagcgtca gcaccagctc ggcgatgccg
     4321 ccgatgatgc tgaggggtgt caccaaggtc aggagtcgaa cgaagcggac tccgcctggg
     4381 cgaggccgcc ctggccggac accgcgatgt tctcaggcga cagcaggaac accttgctgg
     4441 tgacgcgctc gatgcgtccg tacagcccca gcgacagacc gctcgcgaag tcgatcaggc
     4501 gccgcgcatc ggcgtcgctc atctgcgaga ggttgatgat caccggcacg ccctcgcgga
     4561 agttctctgc gatgacctgc gcgtcgcggt actgcttggg gtgcacggtg aggatctcgt
     4621 tcaccgcgcc ggcagccggc tggtgcacgg ccgtggggcg gcgcagcggc gtcacgggtg
     4681 cgggagcgtg ctcctcgcgt tcgcgctcgc gctcacgcgg tgcacgggcc acgggctgcg
     4741 tggcctcctc ctcgtagacc tcttcctcgt cggcgaggcc gagatacacc atggtcttct
     4801 tcagcgggtt acccatcgtg tcctccggtt cgagcgatgc gggctggtct ggttgaaagg
     4861 ttaaccccgg tcggggcggg gtcctgtgat tgcggagccg atccgcaggt gtgtcgcgcc
     4921 tgcatcgatc gcctggacga aatcgccggt catgccggcc gacatccagg tcgcctccgg
     4981 cgcgatgacg cggatgcggt cggcgaccgc acgcaggcgc gcgaatgcgg atgcgggatc
     5041 ctcgtccagc ggggccacgg ccatgacgcc gcggagacgg agcgactcga ggccgagcac
     5101 gt
//
LOCUS       DATMSX010000382         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_157798,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000382 DATMSX010000000
VERSION     DATMSX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157798"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VN041_11715"
     CDS             complement(<1..1043)
                     /locus_tag="VN041_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWU29739.1"
                     /translation="MILQPVLHPVLLVLLLAAPAALVIRALIKGPKAGPGTPGRPIWA
                     LRLGMIIVVFAMLLRPGIPGGTSQTLATDTDIVIVVDTTASIIAEDWDGDKPRLTGVR
                     ADVQSIVSEYPGARFSLISFDAAAQLRLPLTTDATALMSSLDVMLPEITDQSRGSSIS
                     IANRMLADTLSAAAKSSPDRSRMVFYLGDGEQTTSSDPESFASSAKYVDGGAVLGYGT
                     AEGGPMKKTTGRAGANGQGGDYIEYQGERAMSVIDEANLRRIGEQLGVEYQHRTADAE
                     IVLPKAPTTTTDYAASGEVGNVIELYWVLALVLVALIGVELARATMLVTRLRGLADRP
                     ARPPRPSSDKGGAA"
     gene            complement(1040..>1926)
                     /locus_tag="VN041_11720"
     CDS             complement(1040..>1926)
                     /locus_tag="VN041_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWU29740.1"
                     /translation="ESAPAALVSRAERLRSLPSFRASVLRRMSLLSGILIVGAIAVLL
                     GGVVAARPMSAKTIQPINNSRDIMLCLDVSGSMSDVDVEVLSVFEELLKGFKGERIGL
                     TIFNSSPVQVFPLTDDYPFVKEQLQRIRSSFDYNDETPEHWVGTLNGPGASLIGDGLA
                     ACTMRFDHAEEDRSRSVILATDNELAGASILTIEEAAQYAKSKKVRVFALNPVQGKDA
                     EVSAQLENAAALTGGRSYALRDTTTVGDIIAEIQKQEATALKGQAQVVRVDSPNLAIA
                     LTLVSILGFLVLLWRVRL"
BASE COUNT          280 a          643 c          701 g          302 t
ORIGIN      
        1 gcggcgcctc ccttgtcgct cgacgggcgc ggaggacgtg cggggcggtc cgccagtccg
       61 cgcaggcggg tgaccagcat cgtcgcgcgg gcgagctcga cgccgatgag cgcgaccagc
      121 acgagcgcga gcacccagta cagctcgatc acattgccca cctcgccgga ggcggcgtag
      181 tcggtcgtgg tggtcggcgc cttcgggagg acgatctcgg catccgcggt gcggtgctgg
      241 tactccacgc ccagctgctc gccgatccgc cgcaggttgg cctcgtcgat caccgacatg
      301 gcgcgctcgc cctggtactc gatgtagtcg ccgccctgac cgttggcgcc cgcccggccg
      361 gtggtcttct tcatcggccc gccctcggcg gtgccgtagc cgagtactgc cccgccgtcg
      421 acgtacttcg cactgctcgc gaaggattcc gggtcgctgg atgtcgtctg ctcgccgtcg
      481 ccgaggtaga acaccatgcg gctgcggtcg ggcgacgact tcgctgccgc tgagagggtg
      541 tcggccagca tccggttcgc gatgctgatc gacgatccgc gggactggtc ggtgatctcg
      601 gggagcatca cgtcgaggga ggacatcagc gcggtggcat ccgtcgtcag cggcaggcgc
      661 agctgcgccg ccgcgtcgaa gctgatcagc gagaaccgcg cgccggggta ttcggagacg
      721 atcgactgca cgtcggcccg cacgccggtc aggcgcggct tgtcgccgtc ccagtcctcg
      781 gcgatgatgc tggccgtggt gtcgacgacg atcacgatgt cggtgtccgt cgcgagggtc
      841 tgcgaggtgc cgccggggat gccggggcgc agcagcatgg cgaacacgac gatgatcatg
      901 cccaggcgca gcgcccagat cgggcggccg ggggtgccgg ggccggcctt ggggcccttg
      961 atcagggcgc ggatgacgag cgccgccggc gctgcgagca gcagcacgag caggacagga
     1021 tgcaggacgg gctggaggat cacaggcgca ccctccacag cagcacgagg aagccgagga
     1081 tcgagaccag cgtgagcgcg atcgccaggt tggggctgtc cacgcgcacc acctgcgcct
     1141 gacccttcag tgccgtcgcc tcctgcttct ggatctcggc gatgatgtcg ccgaccgtgg
     1201 tcgtgtcgcg caacgcgtag gaccggccgc cggtcagtgc cgcggcgttc tccagctgcg
     1261 cgctgacctc ggcatccttg ccctgcaccg ggttgagggc gaacacgcgc accttcttgg
     1321 acttcgcgta ctgcgcggcc tcctcgatgg tgaggatcga ggcgccggcc agctcgttgt
     1381 cggtggcgag gatgaccgaa cggctgcggt cctcctcggc gtggtcgaag cgcatggtgc
     1441 aggcggcgag cccgtcgccg atcagcgagg cgccggggcc gttcagcgtg cccacccagt
     1501 gctcgggcgt ctcgtcgttg tagtcgaagc tgctgcggat ccgctgcagc tgctccttca
     1561 cgaacgggta gtcgtcggtg aggggaaaca cctgcacggg cgagctgttg aagatcgtca
     1621 acccgatgcg ctcgcccttg aagcccttca gcagctcctc gaacacgctc agcacctcga
     1681 cgtcgacgtc cgacatcgag ccggagacgt ccaggcacag catgatgtcg cggctgttgt
     1741 tgatgggctg gatggtcttg gccgacatcg gccgggcggc gacgaccccg cccagcagca
     1801 cggcgatcgc gccgacgatg aggatgcccg agagcaggct catgcgacgg aggacggatg
     1861 cccggaacga cggcaggctg cgcagccgct cggcgcggga gaccagcgcg gccggtgcgc
     1921 tctcgg
//
LOCUS       DATMSX010000383         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_129330,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000383 DATMSX010000000
VERSION     DATMSX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129330"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VN041_11725"
     CDS             complement(<1..504)
                     /locus_tag="VN041_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU29741.1"
                     /translation="MTSSFPGGTSLSQLDVYDDAASDGVCGGSPHTHLVSTEAYIVIS
                     GEGSLQTIDGAGFRETTLVPGSVVWFTPGTIHRAVNRGGLRVIVLMSNAGLPEAGDAV
                     MTFPSEVVAHAASYAAAASLGDVSDAADERAARAARRRDLAVAGFERLRDAVLDGDRG
                     ALEAFHRA"
     gene            complement(501..1631)
                     /locus_tag="VN041_11730"
     CDS             complement(501..1631)
                     /locus_tag="VN041_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU29742.1"
                     /translation="MLRIGIIGTGGIAQAHVEGYRAFPHECEIVALADALPGRAAEKT
                     VAFGVPDAAAYDDPAEMIARERLDLVSICTPPSTHASLAIAALDAGVNVLVEKPMAPS
                     LEECDAMLAAQERSGRLLSVVAQNRFRDDLATLKEVVDSGLLGSISHVRVDSAWWRGL
                     PYYDLWWRGTWEKEGGGCTLNHAIHHIDLLIWLLGRPDEITAMLANAQHDNAEVEDLS
                     IAALRYETGLAQLTSSVVHHGQEQEIVIQGANARISQPWKVVAERSRPDGFPEEGGDA
                     ALVARIEALIAGRAPLAHRGHEGQIGDVLAAIREGRAPIADGRDGRSAVEVVTAIYKA
                     GFEREHVALPLSAADPYYRAGQLVAHAPHFHEKTASLIEAAS"
     gene            1747..>2669
                     /locus_tag="VN041_11735"
     CDS             1747..>2669
                     /locus_tag="VN041_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU29743.1"
                     /translation="MEKRATSNDVARAAGVSQSTVSFVLTGRAGISEATRERVLRAAA
                     ELNYRPNLAARSMRTRRTGRLAVVVPVASMNPLSLIPGAVAAAQEAGYAVEVVSLPQN
                     PGERGERLAEIVGSGQYEGVLAFTPLHTPPLDEDDPVVLSAGEFDDDMHAAGLFTDAS
                     PIVEMMERLAAMGHTRFLHIAGPQDFPSARARRQAYSDSVQRLGLTSIGVVDGDWSGQ
                     TGVDAIAALPDGILPLAVIAANDLIATGAIQAAMSRGWSVPGDVSITGWDDHPQSAFL
                     VPPLTTVAQDRERLGAHAMARLIAALRGDDAP"
BASE COUNT          399 a          951 c          922 g          397 t
ORIGIN      
        1 ggctcggtgg aaggcctcca gcgcgccgcg gtcgccgtcg agcaccgcgt ctcggagcct
       61 ctcgaatccg gcgaccgcca ggtctcggcg ccgtgccgca cgtgcagccc gttcgtcagc
      121 ggcatccgag acatcgccca gcgatgccgc cgcggcatac gacgcggcgt gcgccacgac
      181 ctccgacggg aacgtcatga cggcgtcacc cgcctcgggg agtccggcgt tgctcatcag
      241 cacgatcacg cgcaggcccc cgcgattcac cgcgcggtgg atcgttcccg gcgtgaacca
      301 cacgaccgat ccggggacga gcgtcgtctc ccgaaagcct gcgccgtcga tcgtctgcag
      361 ggagccttcg cccgagatca cgatgtaggc ctccgtcgac accaggtgcg tgtgggggct
      421 gcccccgcag acgccgtcgg atgcggcgtc gtcgtagacg tccagctgcg acagcgacgt
      481 gccgcccggg aacgacgagg tcatgacgcc gcctcgatca gcgaggccgt cttctcgtgg
      541 aagtgcgggg cgtgcgcgac gagctgccct gcccggtagt acgggtcggc cgccgacagc
      601 ggcagcgcga catgctcccg ctcgaacccg gccttgtaga tcgctgtgac gacctcgacg
      661 gcgctgcgac cgtcgcgtcc gtcggcgatc ggcgcgcgcc cctcgcggat ggcggccagc
      721 acgtcgccga tctggccctc gtggccccga tgggcgagcg gtgcgcggcc cgcgatgagt
      781 gcctcgatgc gcgcgacgag agccgcgtca ccgccttcct cggggaagcc gtccgggcgc
      841 gaccgctcgg cgaccacctt ccagggctgc gagatgcggg cgttcgcacc ctggatcacg
      901 atctcctgct cctgtccgtg gtgcaccacg gaactggtca gctgggccag accggtctcg
      961 tagcgcagcg cggcgatcga gaggtcctcg acctcggcgt tgtcgtgctg ggcgttcgcg
     1021 agcatcgcgg tgatctcgtc cggacggccg agcagccaga tcagcaggtc gatgtggtgg
     1081 atggcgtggt tcagcgtgca cccgccgccc tccttctccc acgtgccgcg ccaccacagg
     1141 tcgtagtacg gcagtcctcg ccaccaggcg gagtccacgc gcacgtggga gatcgatccg
     1201 agcaggcccg aatccacgac ctccttgagc gtggcgaggt catcgcggaa ccggttctgg
     1261 gccaccaccg acagcaggcg tcccgaccgt tcctgggcgg ccagcatcgc atcgcactcc
     1321 tccagcgagg gcgccatcgg cttctcgacg agcacgttca cccccgcgtc gagggcggcg
     1381 atcgcgaggc tcgcgtgggt cgaaggaggc gtgcagatgc tgaccaggtc gagccgttcg
     1441 cgggcgatca tctcggccgg gtcgtcatag gcagcggcat ctggaacccc gaacgcgacg
     1501 gtcttctcgg cggcccgccc ggggagggca tccgcgagag ccacgatctc gcactcgtgc
     1561 gggaacgcgc gatagccctc gacgtgggcc tgcgcgatcc cacccgtgcc gatgattccg
     1621 atcctgagca tccgcgtgcc tctcgttgct aatacgtatt acaaagacgt attaggtagt
     1681 atcggtgaga gcggatcgcg cgtcaagcac gaatcagcgg atgccgatga tcggaagcgg
     1741 agatcgatgg agaagcgtgc gaccagcaac gacgtcgccc gggcggcggg cgtctcgcag
     1801 tcgaccgtgt cgttcgtgct caccggacgg gcgggaatct cggaggccac ccgcgagagg
     1861 gtgctgcggg ccgcggccga gctgaactac cgcccgaacc tcgccgcacg gtcgatgcgc
     1921 acacggcgca caggtcgact ggcggttgtc gtgcccgtcg cgagcatgaa ccccctcagc
     1981 ctgatccccg gcgcggtggc cgcagcgcag gaggccggct atgcggtgga ggtcgtgagt
     2041 ctcccccaga acccgggcga acgaggcgag cgcctcgcgg agatcgtcgg ctcgggtcag
     2101 tacgagggcg tgctcgcctt cacgccgctg cacaccccgc ccctcgacga ggacgacccc
     2161 gtcgtgctct ccgccggcga attcgatgac gacatgcatg ccgcaggcct gttcacggac
     2221 gcctccccca tcgttgagat gatggagcgc ctggcggcca tgggacacac ccgattcctg
     2281 cacatcgccg gtccgcagga cttcccctcc gcgcgtgcgc gccgccaggc gtactcggac
     2341 agcgtgcagc ggctcgggct cacgtcgatc ggtgtcgtcg acggcgactg gagcggccag
     2401 accggggtgg atgcgatcgc cgcgctgccc gacggcatcc tgccgctcgc ggtgatcgcc
     2461 gcgaacgacc tgatcgccac cggcgcgatc caggcggcga tgtcccgtgg atggtcggtg
     2521 ccgggcgacg tcagcatcac cggctgggat gaccatccgc agagcgcttt cctcgtgccg
     2581 ccgctcacca cggtcgcaca ggaccgagag cgcctcggcg cccacgccat ggcccggctc
     2641 atcgccgcgc tgcggggcga tgatgcgcc
//
LOCUS       DATMSX010000384         7659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176494,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000384 DATMSX010000000
VERSION     DATMSX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176494"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VN041_11740"
     CDS             complement(<1..450)
                     /locus_tag="VN041_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWU29744.1"
                     /translation="MSAQGAARPLTLGDLATKKDAREPIVMVTAYDHPSAQIAEQAGI
                     DMVLVGDSAAMTVLGYDSTVPVTIDEMLMLTKAVRRGLSRALLVGDLPFGSYEASDSL
                     AVQTAQRFVKEAGCDLVKIERGGTTVERARAIAAAGIPVVGHVGLTPQ"
     gene            complement(447..872)
                     /gene="panD"
                     /locus_tag="VN041_11745"
     CDS             complement(447..872)
                     /gene="panD"
                     /locus_tag="VN041_11745"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWU29745.1"
                     /translation="MRRTMLKSKIHRATITGSDLNYVGSITIDADLLDASDILEHEQV
                     TIVDVNNGSRFETYTIAGERGTGVIQVNGAAARLVHSGDTIIVISYGDYSAKDLQTYE
                     PTVVHVDRSNRIIKVDDAVDQLLTEALSCDALSDRRETK"
     gene            complement(872..1768)
                     /gene="panC"
                     /locus_tag="VN041_11750"
     CDS             complement(872..1768)
                     /gene="panC"
                     /locus_tag="VN041_11750"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWU29746.1"
                     /translation="MKILRTIPEVRSAVRDARAAGRTVGLVPTMGAFHDGHLSLMRAA
                     RAATDLVVVSLFVNPTQFGPTEDLAAYPRDEARDAALAEQQGVDILFAPEPAEIYPDG
                     FATNIHVAGVTDVLDGATRGAFHFDGVATVVTKLFGIVRPDVAFFGQKDAQQVLVIRR
                     VVRDLDLDVRIEACPIVREPDGLAMSSRNVYLDADARQQATALNRALDAAAQLHASGE
                     RDAARIVAAAERVLADAGIRPDYLELRSNDDLSPLTHVDRDALLAVAARVGAARLIDN
                     HILIAEPAATGIRPEAHQRGGE"
     gene            complement(1765..2520)
                     /locus_tag="VN041_11755"
     CDS             complement(1765..2520)
                     /locus_tag="VN041_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HWU29747.1"
                     /translation="MSTSPDTSEISRTTIAVIGAGRLGGVLANALKRAGFTVAGPLGR
                     DDSIPASDIALLCVPDAEIATAARNARAHATLLGHVSGATPLDDVDFSMHPLQTFTGA
                     ETPEVFQGIGAAIAGCTPHAAHAGEQLARALGARPFHVDDAHRAGYHAASSIASNLVL
                     TVLDAAEQVARAAGIRSDEARELLAPLVTRTVENWRSHGASAALTGPIARGDEQTVAR
                     QRAAISEQTPALLALFDQLCDSARTIATPKGSA"
     gene            complement(2681..7456)
                     /locus_tag="VN041_11760"
     CDS             complement(2681..7456)
                     /locus_tag="VN041_11760"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HWU29748.1"
                     /translation="MANAVESAELRDAIAALEPDVRPDVERLIARLDAIDLAEREARD
                     VVGAAVAMRALAARRGRGETNVSVFTPTLSEHGWTSRRTIVDICTDDAPFLVDSVTSA
                     LAAQGLAVHLLLHPLVEVRRDDDGRLLETDARGGALESWIHLEVDRVPTEEGRDALEE
                     RLLSVLADVHASVDDWSAMRRACLDLVTDLRTAPPATADPAAVGPAVDFFSWLADDNF
                     TFLGYREYSLETDDSGEDVLVPIPHSGLGILRKPTTAVAHLRPEAQRTAREPRLLTIT
                     KANSRATVHRDVYLDYIGARMFDEAGEVIGERRFLGLFTSGAYAASVMTLPIAADKVR
                     AVLDASGFAPMSHSGKDLLGILEQYPRDELFQDSVEHLLKVVTEVTRLNERRRARTFL
                     RRDEFGRFVSVLVYLPKDRYNTPVRLRIEAVLREVFGAEQVDHATRVADSPLAQLHFV
                     VRMPRGTSLPDVAEDDLQLRLADAVRGWDEGLVDALHHAHGDDEAARLLADYGQAFPA
                     VYKETVTPDEAVGDVALLEQLREQEFAVRLNVPEKDVPGKRVLTLASREEYPLTRVLP
                     VLTDLGVDVVDERPYTISLPDGTTWHVSDFGLTAEGVDRWTDPAWGAMFEDAFSATWT
                     GAAESDRLNTLVLHGGLDWRRIVILRAIAMYLRQAGSAFSVEYTENALVSNPGLAATL
                     VRLFELRFDPSVEGDRDAQIESAQAELLEGLDQVASLDDDRILRSFDGVIAATWRTNF
                     YQPAADGTPKPWVSMKLDCARVPGLPKPLPMAEIWVYSPEVEGVHLRFGKVARGGLRW
                     SDRREDFRTEVLGLVKAQMVKNAVIVPTGSKGGFFAKRLPAPGDRAAWLEAGKSAYRT
                     FIRGLLDLTDNRVGSDVVPPADVVRHDGDDPYLVVAADKGTASFSDIANGISEEYGFW
                     LADAFASGGSAGYDHKAMGITARGAWESVKRHFRELGLDTQTEDFTVVGIGDMSGDVF
                     GNGMLRSRHIRLVAAFDHRHVFIDPNPDAESTFAERERLFALPGSSWDDFDRSVISAG
                     GGVYPLSMKSIAVTPEMAEALALDPAVTKMTPMELKKAVLLAPVDLLWNGAIGTYIKA
                     SDESDAEVGDRGNDAVRVDGRELRLRVVGEGGNLGVTQRGRIEAASAGIRINTDAIDN
                     SAGVGTSDREVNIKILLGILEREGRLDRPARNELLQSMTDEVAVQVLRDNYEQNVLLG
                     NSRANAGVMLPVHERLMEWLEERDELDRELEFLPSRAKVADLIADQQGLTRPEFAVLV
                     AYAKLALKADLAATGLADDPWFERTLAEYFPAPIREAYAGDLDAHPLRTEIIVNTVVN
                     SMVNRGGITFAYRAADETGATSEDIARAYVAVREIFDLRGFVVAVEATDNVVSTDVQT
                     DLYLTFRRLLDRATRWFVQHRPDGVDIGAEIELFREPVAHLSSNVDTWLQGVDLERFE
                     SRTSELQAAGVPAELARRGAGLLDVLSLLDIAECARTRGWTLEGVAGLYFALSARLAF
                     EQTLTKITALPQTDRWGSMARASMRDDLYAVMIELTATVAGQTEADAPEARIEAWLEQ
                     GGASTRRTVDEAIAAATAQDGSGLATLSVAVRRLRSLVR"
     gene            complement(7550..>7659)
                     /locus_tag="VN041_11765"
     CDS             complement(7550..>7659)
                     /locus_tag="VN041_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWU29749.1"
                     /translation="ACRRANAAAAIKVTRRGPTTAPTAAEVDAFLRDRS"
BASE COUNT         1095 a         2517 c         2861 g         1186 t
ORIGIN      
        1 ctgcggcgtg aggccaacgt gcccgaccac ggggattccg gcggcggcga tggcacgcgc
       61 ccgctcgacc gtggtgccgc cgcgctcgat cttgacgagg tcgcagccgg cctccttcac
      121 gaagcgctgc gcggtctgca cggcgaggga atcggatgcc tcgtacgacc cgaacggcag
      181 gtcgccgacg agcagggcac gggacagccc gcgccgcacc gccttcgtga gcatgagcat
      241 ctcgtcgatc gtgaccggca ctgtcgagtc gtatccgagc acggtcatcg ccgcggagtc
      301 gccgaccagc accatgtcga tgccggcctg ctcggcgatc tgcgcgctgg ggtgatcgta
      361 ggccgtgacc atgacgatcg gctcacgggc atccttcttc gtcgccaggt caccgagggt
      421 cagcgggcgc gcggcgccct gcgcgctcac ttcgtctccc ttcggtcgct cagtgcatcg
      481 catgacaggg cctcggtgag cagctgatcc acggcgtcgt cgaccttgat gatccggttg
      541 gagcggtcga cgtgcacgac ggtcggctcg taggtctgca ggtccttcgc ggagtagtcg
      601 ccgtaggaga tgacgatgat cgtgtcgccg gagtgcacca ggcgggcggc ggcgccgttc
      661 acctggatca cgccggtgcc gcgctcgccc gcgatcgtgt aggtctcgaa gcgcgagccg
      721 ttgttcacgt cgacgatcgt cacctgctcg tgctcgagga tgtcggacgc gtcgagcagg
      781 tcggcgtcga tcgtgatgga gccgacgtag ttcaggtcgc tgccggtgat ggtggcgcgg
      841 tggatcttcg acttcagcat ggtgcgtcgc atcattcgcc gcctctctga tgggcttcgg
      901 ggcggatgcc ggtggcggcc ggctccgcga tcaggatgtg gttgtcgatc aggcgcgcgg
      961 cgcccacgcg ggcggcgacc gcgagcaggg cgtcgcggtc gacgtgggtg agcgggctga
     1021 ggtcgtcgtt cgagcgcagc tcgaggtagt cgggtcggat gccggcatcc gcgaggaccc
     1081 gttccgccgc ggccacgatc ctcgcggcat cccgctcgcc actcgcgtgg agctgcgccg
     1141 cggcgtcgag ggcgcggttc agcgcggtgg cctgctggcg ggcatccgca tcgaggtaga
     1201 cgttgcgcga gctcatggcg agtccgtcgg gctcgcggac gatcgggcac gcctcgatac
     1261 gcacatcgag gtcgagatcg cggacgacgc gtcggatcac gagaacctgc tgggcgtcct
     1321 tctggccgaa gaaggcgacg tcggggcgga cgatgccgaa cagcttggtg acgacggtgg
     1381 cgacgccgtc gaagtggaag gcgccgcggg tggcgccgtc gagcacgtcg gtgacccccg
     1441 cgacgtggat gttcgtggcg aagccgtcgg ggtagatctc ggcgggttcg ggggcgaaga
     1501 ggatgtcgac gccctgctgc tcggcgagcg cggcgtcacg ggcctcgtcc cgcgggtagg
     1561 cggcgaggtc ctcggtgggg ccgaactggg tggggttgac gaacagcgag acgacgacga
     1621 ggtcggtcgc cgcgcgcgcg gcgcgcatga gggagagatg cccgtcgtgg aaggcgccca
     1681 tcgtggggac gaggccgacg gtgcggcctg cggcgcgtgc atcgcggacg gcggagcgga
     1741 cctcggggat ggtgcggagg atcttcatgc ggagcccttc ggggtcgcga tggtgcgtgc
     1801 gctgtcgcag agctggtcga acagggcgag aagcgcgggg gtctgctcgg agatcgcggc
     1861 gcgctggcgg gcgacggtct gctcgtcacc gcgggcgatc ggaccggtga gggctgcgct
     1921 cgccccgtgg gaacgccagt tctcgacggt gcgggtgacg agcggtgcga gcagctcgcg
     1981 cgcctcgtcc gagcggatgc ccgcggcacg ggcgacctgt tcggcggcat cgagcacggt
     2041 gagcaccagg ttcgaggcga tcgacgaggc agcgtggtag ccggcgcggt gcgcgtcgtc
     2101 gacgtggaac gggcgggcgc cgagggctcg ggcgagctgc tcgcctgcgt gtgcggcgtg
     2161 cggagtgcac cccgcgatgg cggcgccgat cccctggaac acctcaggag tctcggcgcc
     2221 ggtgaacgtc tgcagcggat gcatgctgaa gtcgacgtcg tcgaggggcg tggcgccgga
     2281 gacgtggccg agcaaggtcg cgtgcgcccg ggcgttcctc gcggctgtcg cgatctcggc
     2341 atccgggacg cagagcaggg cgatgtcgga tgccgggatg ctgtcgtccc ggccgagcgg
     2401 acccgccacg gtgaatccgg cccgcttcag cgcgttcgcg agcacgccac cgagacggcc
     2461 cgcgccgatg acggcgatgg tcgttcggga gatctcggag gtgtccggag aggtgctcat
     2521 gacgggatga agtatccgcc tggcatcgat gtcaggcaaa atctgggatc aatacatcaa
     2581 gccgactcgc tccagggatg gtcgttctga ttcagaagcg ctaacactgc ttgtcatctt
     2641 gatctcacac gagaccgcgg cgcaggcatc cgtccttcgc tcaccgcacc agcgaacgca
     2701 gcctccgcac cgcgaccgag agcgtcgcca gcccggagcc gtcctgcgcc gtcgccgcgg
     2761 cgatggcctc gtcgacggtt cgtcgcgtcg acgcaccgcc ctgctccagc cacgcctcga
     2821 tccgcgcctc cggtgcgtcc gcctcggtct gacccgcgac cgtcgcggtc agctcgatca
     2881 tcacggcgta caggtcgtcg cgcatgctcg cccgcgccat cgacccccat cggtcggtct
     2941 gcggcagcgc ggtgatcttg gtcagcgtct gctcgaacgc cagccgcgcg gacagcgcga
     3001 agtagagtcc cgcgacgccc tcgagcgtcc agccccgcgt gcgcgcgcac tccgcgatgt
     3061 cgagcagcga gagcacgtcg agcagcccgg caccgcgcct ggccagctcg gcaggaacgc
     3121 ccgccgcctg cagctcgctc gtgcgcgact cgaagcgctc cagatcgacg ccctgcagcc
     3181 acgtgtccac gttcgacgac aggtgcgcga cgggttcccg gaacagctcg atctcggcgc
     3241 cgatgtcgac accgtcgggc cgatgctgca cgaaccagcg ggtggcgcgg tcgagcaggc
     3301 ggcggaaggt gaggtacaga tcggtctgca cgtcggtcga gacgacgttg tcggtggcct
     3361 cgaccgcgac gacgaagccg cgcaggtcga agatctcgcg caccgcgacg tacgcgcggg
     3421 cgatgtcctc gctcgtcgcg ccggtctcgt ccgccgcccg gtaggcgaag gtgatgcctc
     3481 cgcggttcac catcgagttc accacggtgt tgacgatgat ctcggtgcgc agcggatgcg
     3541 cgtccagatc accggcgtac gcctcgcgga tcggcgcggg gaagtactcg gcgagcgtgc
     3601 gctcgaacca cggatcgtcg gccagcccgg tggctgcgag gtcggccttg agagccagct
     3661 tcgcataggc gacgagcacc gcgaactcgg ggcgggtgag gccctgctgg tcggcgatca
     3721 ggtcggccac cttcgcccgg ctgggcagga actccagctc gcgatcgagc tcgtcgcgct
     3781 cctcgagcca ttccatcaac cgctcgtgca cgggcagcat gacaccggcg ttggcgcggg
     3841 agttgccgag cagcacgttc tgctcgtagt tgtcgcgcag cacctgcacc gcgacctcgt
     3901 cggtcatcga ctgcagcagc tcgttgcgcg ccgggcgatc gagccgcccc tcgcgctcga
     3961 ggatgccgag caggatcttg atgttcacct cgcggtcgga ggtgccgacg ccggcggagt
     4021 tgtcgatcgc gtcggtgttg atccggatgc ccgcggatgc cgcttcgatg cggccgcgct
     4081 gcgtgacccc gaggttgccg ccctcgccga ccacccgcag gcgcagttct cggccgtcga
     4141 cgcggacggc gtcgttgccg cggtcgccga cctcggcgtc gctctcgtcg ctcgccttga
     4201 tgtaggtgcc gatggcgccg ttccagagca ggtcgaccgg cgcgagcagc acggccttct
     4261 tcagctccat cggggtcatc ttcgtgaccg ccgggtcgag cgcgagcgcc tcggccatct
     4321 cgggtgtgac ggcgatcgac ttcatcgaca gcgggtacac gccacccccg gcggagatca
     4381 cgctgcggtc gaagtcgtcc cacgacgaac cggggagggc gaacaggcgc tcccgctccg
     4441 cgaaggtgga ctcggcgtcc gggttcggat cgatgaacac gtggcggtgg tcgaaggccg
     4501 cgaccagccg gatgtggcgc gagcgcagca tcccgttgcc gaagacgtcg cccgacatgt
     4561 ctccgatgcc gacgacggtg aagtcctcgg tctgcgtgtc cagtccgagc tcgcggaagt
     4621 gccgcttgac cgactcccag gcgccacggg cggtgatgcc catggccttg tggtcgtagc
     4681 ccgccgagcc gcccgaggcg aaggcatccg caagccagaa tccgtactcc tcggagatgc
     4741 cgttggcgat gtccgagaac gacgcggtgc ccttgtcagc cgcgacgacc aggtaggggt
     4801 cgtcgccgtc gtgccgcacg acatcggccg gcggcacgac gtcggagccg acccggttgt
     4861 cggtgaggtc gagcaggccg cggatgaacg tgcggtaggc ggacttgccg gcctcgagcc
     4921 acgcggcgcg gtcgccgggg gcgggcaggc gcttggcgaa gaagccgccc ttggaaccgg
     4981 tcgggacgat gacggcgttc ttcaccatct gcgccttgac caggccgagc acctcggtgc
     5041 ggaagtcctc gcgccggtcg ctccagcgca gcccgccgcg cgcgaccttt ccgaaccgca
     5101 ggtgcacacc ctcgacttcg ggcgagtaca cccagatctc ggccatcggc agcggcttgg
     5161 gcagcccggg gacccgggcg cagtcgagct tcatcgacac ccacggcttg ggcgtgccgt
     5221 cggccgcggg ctggtagaag ttggtgcgcc aggtcgccgc gatgacaccg tcgaacgagc
     5281 gcaggatgcg gtcgtcgtcg aggctcgcga cctgatcgag tccctcgagc agctcggctt
     5341 gggcggattc gatctgcgcg tcgcggtcgc cctcgacgga cgggtcgaag cgcagctcga
     5401 acaggcgcac cagggtggcg gccagtcccg ggttcgagac gagtgcgttc tcggtgtact
     5461 ccacggagaa cgccgacccg gcctggcgca agtacatcgc gatcgcgcgc aggatgacga
     5521 tgcgccgcca gtcgaggccg ccgtgcagca cgagggtgtt gagccgatcg ctctcggcgg
     5581 cgcccgtcca ggtggcgctg aacgcgtctt cgaacatcgc gccccaggcg gggtcggtcc
     5641 accgatccac gccctcggcg gtgaggccga agtccgagac gtgccacgtc gtgccgtcgg
     5701 ggagcgagat cgtgtacggg cgctcgtcga cgacgtcgac gcccagatcg gtgagcacgg
     5761 ggagcacgcg ggtgagcggg tactcctccc gggatgcgag ggtgaggacg cgcttgccgg
     5821 ggacgtcctt ctcgggcacg ttgagccgca ccgcgaactc ctgctcgcgc agctgctcga
     5881 gcagggctac gtctccgacc gcctcgtcgg gggtgaccgt ctccttgtac accgcgggga
     5941 aggcctgacc gtagtcggcg agcaggcggg cggcctcatc gtcgccgtgg gcgtggtgca
     6001 gggcgtcgac gagaccctcg tcccatccgc gcacagcatc cgccagcctg agctgcaggt
     6061 cgtcctcagc gacgtcgggc agggaggtgc cgcgcggcat gcgcacgacg aagtgcagct
     6121 gggcgagcgg cgagtcggcc acgcgggtgg cgtggtcgac ctgctcggcg ccgaacacct
     6181 cccgcagcac ggcttcgatg cgcagtcgca cgggggtgtt gtagcggtcc ttgggcagat
     6241 agacgagcac cgagacgaag cggccgaact cgtcgcgccg caagaaggtg cgggcgcggc
     6301 gacgctcgtt caggcgggtg acctcggtca ccaccttgag caggtgctcg acggagtcct
     6361 ggaacagctc gtcgcggggg tactgctcga ggattcccag caggtccttg ccggagtgcg
     6421 acatgggagc gaatcccgag gcgtcgagca cggcgcgcac cttgtcggcg gcgatcggca
     6481 gggtcatgac ggatgccgcg tacgcacccg acgtgaacag gcccaggaac cggcgctcgc
     6541 cgatgacctc gcccgcctcg tcgaacatgc gtgcgccgat gtagtcgagg tagacgtcgc
     6601 gatgcaccgt ggcgcgcgag ttcgccttcg tgatcgtgag caggcgcggc tcgcgggcgg
     6661 tgcgctgcgc ctcaggacgc aggtgggcga cagccgtggt gggcttgcgc aggatgccga
     6721 gcccggagtg cgggatcggt accagcacgt cctcgccgct gtcgtcggtc tccagggagt
     6781 attcgcggta gccgaggaag gtgaagttgt cgtcggcgag ccagctgaag aagtcgacgg
     6841 cagggcctac ggcggcggga tcggccgtgg ccggcggcgc ggtgcgcagg tcggtgacca
     6901 ggtcgaggca ggcgcggcgc atcgcgctcc agtcgtcgac cgaggcgtgc acgtcggcga
     6961 gcaccgacag cagtcgctct tcgagggcgt cgcggccctc ttcggtgggg acgcgatcga
     7021 cctcgaggtg gatccacgat tcgagcgcac cgccccgggc atccgtctcg agcagtctgc
     7081 cgtcgtcgtc gcggcgcact tcgaccagcg gatgcagcag cagatgcacc gcgagcccct
     7141 gggcggcgag cgcggaggtg accgagtcga cgaggaacgg ggcgtcgtcg gtgcagatgt
     7201 cgacgatggt gcggcgcgac gtccagccgt gctcgctgag cgtgggggtg aagaccgaca
     7261 cgttcgtctc gccccggcct cggcgggcgg ccagagcgcg catcgcgact gcggcaccca
     7321 cgacatcgcg ggcttcgcgc tcggccagat cgatcgcgtc gagccgggcg atcaggcgct
     7381 ccacatcggg tctgacgtca ggttcgagtg ccgcgatggc gtctcggagt tcggcggatt
     7441 cgactgcgtt cgccatgctg cgcgttcccc ttcagcggtg gtgcgtgggt cgtcttcgac
     7501 ctcctgaaga gcctacccgt cggggtgggg gggacagtgt ccgggtgtct cagctgcggt
     7561 cgcgcaggaa cgcgtcgacc tcggccgcgg tgggcgcggt cgtcgggcct cggcgggtga
     7621 ccttgattgc ggcggcggcg ttggctcgcc ggcaggcat
//
LOCUS       DATMSX010000385         7582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_185962,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000385 DATMSX010000000
VERSION     DATMSX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185962"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VN041_11770"
     CDS             complement(<1..1044)
                     /locus_tag="VN041_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29750.1"
                     /translation="MTADLVVDVLGLRVLIEPGDALDAAHHKRLRAAWSGAISADAAT
                     TSDLTVSFNAEASFDQGMEQLTVDVTLAALEALRGRALMFHAAGVADAEGRVVAFVGP
                     SGRGKTTLSRALGRRFGYVSDETVAADESRSVHPYRKPLSVVRKDLPKEQVSPVDAGL
                     LPVPAVPLRLDGLVLIDRDPELDAPQLSTVPLAEALPELVTQMSYLRDHPAPLQSIAR
                     LCDAVGGVRMLRYPDAETVPAIMPEILGAEPAASEWHPLALPAASGPYGVAQARDAIR
                     SDDYLIVMVESELKVLDGIAPVIWQAAADGADLDGIIAAVLDAHGEPPTGAARQIVED
                     AVDELVVAGVLSRD"
     gene            complement(1041..2561)
                     /locus_tag="VN041_11775"
     CDS             complement(1041..2561)
                     /locus_tag="VN041_11775"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWU29751.1"
                     /translation="MELRDYLRIFRAHWLGMLAVIVGAVVLAFGWTLTQPKVYTADAT
                     ALVRTTGGDELGTVSLGNSIASQRVKTYVEIGQSRSVADRVITELDLAKSPDSLINQV
                     SITNPIDTTSLKVTANGSSPEQSRDLAEAWVRAMIAEVNKIESGDPDKTGSVFLSAVD
                     SARLPSSPSSPNTKLALAIGALVGLALAIGYGLLRYTLDRRIRDVAAVERETGVAVVG
                     TVPEEKSFTADDRLIPFDGGNSANSSKSHLFAIAEAMRELRTNIQFMDVDNPPRVMVI
                     TSPLPGDGKSTTASNLAITLASSGQKVVLIDGDLRRPMIDTVFGLPKGAGLSDVLAGR
                     AELADVAHRVGGNGRLLVIGAGKIPPNPSEVLGSARMKELLEAISREAIVIIDAPPLI
                     PVTDAAVLAHSTDGAIVVATVGKTTFEVLGKALSNLERAGARALGIVLNRVPRRGSGA
                     AYYGYQYHGDYYRVEKPEDEADQLLASTPTSEPVRSEPAARDDDGAPLRRSTHRAS"
     gene            complement(2719..3357)
                     /locus_tag="VN041_11780"
     CDS             complement(2719..3357)
                     /locus_tag="VN041_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29752.1"
                     /translation="MNENTTARAPRTIDRRTVVKGAAWSVPAIAAAVAMPLASASGTP
                     ECPGCMKAGVGAFTLAGVAASSRATLAFAAPVILDATDCGTLIGNIFDFKPAFTYIVT
                     SATLAMSDGATYNSLIGLAPGAGVLGAVGAMAAAFSFTNVRVDPSNTRTGLPRTKPVS
                     LTVQINVTFKWGLGVELLCPVTLVYDLSSAVNVSVLAAGIGAVSYTGFTAAA"
     gene            complement(3514..4389)
                     /locus_tag="VN041_11785"
     CDS             complement(3514..4389)
                     /locus_tag="VN041_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU29753.1"
                     /translation="MGNLIDVAAVMRQLGPGSEKPEAVCRHRSTLATSYTFDGGARWS
                     WRRQPTGRDALAGLVFVDVPVTVDGDLGEERAEVGSAVFLHPHRDLHLSAQARGKAVG
                     VWLPWDSLLEVETGVQSPRQVLTPTPLTVGLRAFLTSLLVQPSDATPYTDYLVERVLA
                     EMAFGALLESVPKNVAGAREDRPIDRARSLMLMRRAEGEFGVAELAQEMHMSTRHLQR
                     MFAAENSSPADELRGMRIDLANELLGDPAYDSLSISEIAMHAGLRTPSALRRAFAGRG
                     LPLPARARSERMTVG"
     gene            4561..5277
                     /gene="lepB"
                     /locus_tag="VN041_11790"
     CDS             4561..5277
                     /gene="lepB"
                     /locus_tag="VN041_11790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWU29754.1"
                     /translation="MTSADDASPAPRPLWRRITGSTWFHLILAFVVVGAVLSFVAKPY
                     AVPSGSMEQTLTPGDRALVNRLAFVGGGPSTGDIVVFDADQTWGPDGSADDGPLKGVL
                     RWIGEVTGFGPSGPHTLIKRVIAGPGQTVSCCDAEGRLLVDGEPLDEPYVFEDIPFTP
                     GSLDCTTTPASLRCLPEVTVPAGSYLVLGDHRSNSADGAYPCRAPDADAATCYRWAKR
                     EDIVGKAVAILWPVGRWSGL"
     gene            5454..6968
                     /locus_tag="VN041_11795"
     CDS             5454..6968
                     /locus_tag="VN041_11795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWU29755.1"
                     /translation="MAVSEPWGAHWGAIGEASQTPAVAFAPPASTSAEWQAAYRRRLV
                     VTDAIIVVVAVFGAQFLRFGEDSASLYIPGFSGSSFTLAYTLVSAVFAVAWLVGLRLH
                     GTRDPKTVGNGTQEYRSIADASIRTFGILTIAAFLLHIEVARAYLLLALPVGLLLLIA
                     GRWMWRGWLSAQRSQGRFLTRIVIVGEHAKSVHIAREVQRDHTAGYEIIGAVTERGHG
                     LLLPGIPVIGDLDGMLPALEAVGADAVVYSGSDLISPPRLRQFGWELQTRSIDLIVAA
                     ALTDIAGPRIHARPVAGLSLIHVDYPEFTGMRYATKRAFDVCVSALALLVLSPLLLAI
                     AFAVRTDGGPALFVQERIGLDGRRFRLLKFRSMVPDAEDMLSNLLAFSDGNGVLFKMR
                     DDPRVTRVGGVLRRFSLDELPQLWNVLRGDMSLIGPRPPLMREVQNYEDFAQRRLLVK
                     PGITGLWQVSGRSDLSWEDSVRLDLYYVENWSLTGDLVILWRTVRTLLRPHGAY"
     gene            complement(6997..>7582)
                     /locus_tag="VN041_11800"
     CDS             complement(6997..>7582)
                     /locus_tag="VN041_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29756.1"
                     /translation="TPPLGGVAAVATWLWPWTLGLAAVAVTFVLVNQPGAPEVVTAAP
                     AGIADEELGEYLRTRTPAVPVTRGDGAVMNLRALSMEKPILLLSVSETCGSCTPVIER
                     ADAYRELLPEASVQLLVRSTPEASPMASATEPFTLHDPDGYVRDSLEMPRTPSAVLLG
                     ADGLLAGGPVVGHPDIEEFVADIYESLHGTRPAA"
BASE COUNT         1120 a         2617 c         2653 g         1192 t
ORIGIN      
        1 gtcgcgactg agcacgccgg cgacgacgag ctcgtccacg gcgtcctcga cgatctgccg
       61 cgccgcaccc gtcggaggct cgccgtgcgc gtccagcacg gcggcgatga tgccgtcgag
      121 atcggcgccg tcggctgccg cctgccagat gacgggcgcg atgccgtcga gaaccttcag
      181 ctcggactcc accatcacga tcaggtagtc gtcggatcgg atggcgtccc gcgcctgcgc
      241 gacgccgtac ggaccgctcg cggccggcag agccagcgga tgccattcgg atgcggcggg
      301 ttctgcgccg aggatctcag gcatgatggc gggcacggtc tcggcgtcgg gatagcgcag
      361 catccgcacg cccccgaccg catcgcacag gcgggcgatc gactgcagcg gggcggggtg
      421 gtcgcgcagg tagctcatct gcgtcaccag ttcgggcagc gcttcggcca gcggcacggt
      481 gctcagctgc ggggcgtcga gctccggatc acggtcgatc agcacgagtc cgtcgagccg
      541 cagcggcaca gcgggcaccg ggagcagccc tgcgtcgacg ggcgagacct gctccttggg
      601 gaggtccttg cgaaccacgg acagcggctt gcggtacggg tgcacggacc gggactcgtc
      661 cgcggcgacg gtctcgtcgg acacgtagcc gaaccgacgt cccagcgccc ggctgagggt
      721 cgtcttgccg cgcccggagg ggccgacgaa cgcgacgacg cgcccctcgg cgtccgccac
      781 acccgccgca tggaacatca gcgcgcgtcc ccgcagggct tccagcgccg cgagcgtcac
      841 gtcgaccgtc agctgctcca tcccctggtc gaaggatgcc tcagcgttga aggacacggt
      901 cagatcggac gtcgtcgccg catccgcact gatcgcaccg ctccatgccg cgcggagccg
      961 cttgtggtgt gcggcgtcga gagcgtcccc gggctcgatc aggacgcgca ggccgagcac
     1021 gtcgacgacc aggtccgccg tcatgacgcg cgatgcgtgc ttctgcgcag cggagccccg
     1081 tcatcatcgc gtgccgccgg ctcactgcgc acgggctcgc tggtcggcgt gctcgcgagc
     1141 agctggtcgg cttcgtcctc aggcttctcc acgcggtagt agtcgccgtg gtactggtag
     1201 ccgtaatagg cggcgccgct tccgcgccgc gggacgcggt tgagcacgat gcccagggca
     1261 cgggcgccgg cgcgctcgag gttgctgagc gccttgccga gcacctcgaa ggtggtcttg
     1321 ccgacggtgg cgacgacgat cgcgccatcg gtgctgtggg cgaggacggc ggcgtcggtc
     1381 acggggatca gcggcggggc gtcgatgatc acgatcgcct cgcgggagat cgcctcgagc
     1441 agctccttca tgcgcgcgga gccgagcacc tcgctcgggt tcggcgggat cttgcccgct
     1501 ccgatgacca gcagccgccc gttgccgccg acgcggtgcg cgacatcggc cagctcggca
     1561 cgcccggcca gcacgtcgga gaggccggcg cccttgggca ggccgaagac cgtgtcgatc
     1621 atgggacgac gcaggtcgcc gtcgatcagg acgaccttct ggccgctgga ggcgagggtg
     1681 atcgccaggt tcgaggcggt ggtcgatttg ccgtcacccg gaagcgggct ggtgatgacc
     1741 atcacccgcg gcgggttgtc gacgtccatg aactggatgt tggtgcgcag ctcgcgcatc
     1801 gcctcggcga tcgcgaacag gtgcgacttg gagctgttgg cggagttgcc gccgtcgaac
     1861 gggatgagac ggtcgtcagc ggtgaacgac ttctcctccg ggacggtgcc caccaccgcg
     1921 acgccggtct cgcgctccac cgcggcgaca tcgcggatgc ggcgatcgag ggtgtagcgc
     1981 agcaggccgt agccgatcgc gagggcgagg cccacgaggg cgccgatcgc gagtgcgagc
     2041 ttggtgttgg gcgaggaggg gctgctggga aggcgggccg aatcgaccgc gctgaggaac
     2101 accgagccgg tcttgtcagg atcgccggat tcgatcttgt tgacctcggc gatcatcgcg
     2161 cgcacccagg cctcggcgag atcgcgggac tgctccgggc tcgagccgtt cgccgtcacc
     2221 ttcagcgagg tggtgtcgat cgggttcgtg atgctgacct ggttgatcag cgagtcgggc
     2281 gacttcgcca ggtcgagctc cgtgatcacg cggtcggcga ccgagcggct ctgcccgatc
     2341 tcgacgtagg tcttgacacg ctgcgaggcg atgctgttgc cgaggctgac cgtccccagc
     2401 tcatctccgc ccgtcgtccg cacgagagct gtggcgtccg ccgtgtacac cttgggctgc
     2461 gtcagcgtcc agccgaaggc gagcacgacg gcgcccacga tgacggccag catgcccagc
     2521 cagtgtgcgc ggaagatccg caggtagtcc ctcaactcca tgcgcgcgtg cacccctcag
     2581 tccgtacgac gcaataatgc cttcgaatct agcagtcgga tgccgcgccg acaggcgttc
     2641 cctggcgaca tccgggcatg aatgagcgac gggactcctt ccggagaagg agtcccgtcg
     2701 tctgacgcgg atgctgggtc aggctgcggc ggtgaaaccc gtgtagctga cggcaccgat
     2761 acccgcggca aggaccgaca cgttgacggc cgacgagagg tcgtacacga gagtcacggg
     2821 gcagagcagc tccacgccca ggccccactt gaacgtcacg ttgatctgga cggtcagcga
     2881 gacgggcttg gttcgcggca agcccgtgcg ggtgttgctc gggtccaccc gcacgttcgt
     2941 gaacgagaag gcggcagcca tcgccccgac cgctcccagc acgccggcac caggagcgag
     3001 gccgatcagc gagttgtagg tcgctccatc gctcatcgcg agagtcgcgc tggtcacgat
     3061 gtaggtgaac gccggcttga agtcgaagat gttgccgatc agcgtgccgc agtcagttgc
     3121 atcgaggatc accggcgcgg cgaacgcaag ggtggcacgg ctgctggctg cgacgccggc
     3181 cagcgtgaag gcgcctacac ctgccttcat gcagcccggg cattcaggcg ttcccgaggc
     3241 cgatgccagc ggcatggcga cggcggcggc gatggcggga accgaccacg cggctccctt
     3301 gacgaccgta cggcggtcga tcgtgcgcgg tgctcgcgcg gtggtgttct cattcatttg
     3361 ctgtgtttcc cctcaacaag cagtacccag tggtgtcggg cggcttctca gggccgcaca
     3421 ccatcgatga tctgagcgcc ggggagagcg ctcaacgcgc ctgtcgcact tttcacgagt
     3481 cctgtcgcaa agcggatctc ttcgcccggg gcctcagccc accgtcatcc gctcactccg
     3541 ggcgcgagcc ggaagcggga gtccgcggcc ggcgaatgcg cgccggagcg ccgacggcgt
     3601 gcgaagacct gcatgcattg cgatctcgct gatgctcagc gagtcgtacg ccgggtcacc
     3661 gagcagctcg ttggcgagat cgatgcgcat gccacgcagc tcatcagccg gcgagctgtt
     3721 ctccgccgcg aacatccgct gcaggtggcg agtcgacatg tgcatctcct gcgcgagctc
     3781 ggcgacgccg aactccccct ccgcacggcg catcagcatc agcgaacggg ctcggtcgat
     3841 gggcctgtcc tcgcgagcgc ctgccacgtt cttcggcacc gactcgagca gcgcgccgaa
     3901 cgccatctcg gcgagcacgc gctccaccag atagtcggtg tacggcgtcg cgtccgacgg
     3961 ctgcacgagc agagaggtca ggaaggcccg cagcccgacg gtcagcggcg tcggcgtcag
     4021 cacctgccgg ggcgactgaa cgcccgtctc gacctcgagc agcgagtccc acggcagcca
     4081 gaccccgacg gccttgccgc gggcttgagc cgaaaggtgc aggtcgcgat gcggatgcag
     4141 gaacacggcg ctcccgacct cggcgcgctc ctcgcccagg tcgccgtcga ccgtgacagg
     4201 gacgtcgacg aacacgagcc ccgccagtgc gtcccgcccc gtcggctgcc gtcgccacga
     4261 ccagcgcgcg cccccatcga aggtgtagct cgtcgcgagc gtgctgcgat ggcggcatac
     4321 cgcctcgggc ttctccgagc ccggccccag ctgccgcatg accgcggcga cgtcgatcag
     4381 attccccatg acggacctcc ccagatcctc gacacgcccg gccccatccg tcagcgttcg
     4441 ttcgggacag catagacccg catgggccat tccgcgacac tgaacgcacg aagatgcgac
     4501 ctcgccggcg atcggcgtcc actcccggag tcgatcagca aggtggggca tgctgtatgc
     4561 gtgacctccg cagacgacgc atcccctgcc ccccgcccac tgtggcggcg gatcacgggc
     4621 agcacgtggt tccacctgat cctggcgttc gtggtggtcg gggcggtgct gtcgttcgtg
     4681 gcgaagccct acgctgtgcc gtcgggttcc atggagcaga ccctcacccc cggtgatcgg
     4741 gcgctcgtca accggctcgc gttcgtgggt gggggcccca gcacgggcga catcgtcgtg
     4801 ttcgatgcgg accagacctg gggtccggac ggcagcgccg acgacggtcc gctcaaaggg
     4861 gtgctgcgct ggatcggtga ggtcacggga ttcggaccct ccggtccgca caccctcatc
     4921 aagcgggtca tcgcggggcc gggccagacc gtctcctgct gcgacgccga gggccggctg
     4981 ctggtcgacg gcgaacccct cgacgagccg tacgtgttcg aggacatccc cttcacaccg
     5041 ggctctctgg actgcaccac cactccggcc tcgctccgct gcctgcccga ggtgaccgtg
     5101 cccgccggct cgtatctggt gctgggcgac caccgttcga actccgccga cggcgcgtac
     5161 ccctgccggg ctccggacgc cgatgcggcc acctgttatc gctgggcgaa acgcgaggac
     5221 atcgtcggga aggccgtcgc gatcctctgg ccggtcggcc gatggagcgg tctctgaggg
     5281 ttcggcgcag accgatatat ccggggctgt acgtctgggg tttgacggct tagactgcag
     5341 acagtcctcc tgtgggagga ggatgacgtc gcttgctggg gcagcgatga agcgctgggt
     5401 gcacgatccg tcgtgtgcgc tgcgcgtgag acgaaatcct ctggggaggg atcgtggccg
     5461 tatccgagcc atggggtgcg cactggggtg cgataggaga ggcgagtcag acgcctgcgg
     5521 tggcgttcgc gccgcccgcg tcgacaagcg cggagtggca ggcggcgtac cgtcgtcgcc
     5581 tggtcgtcac ggacgcgatc atcgtcgtgg tggcggtctt cggcgcgcag ttcctgcgct
     5641 tcggtgagga ctccgccagc ctgtacatcc cgggcttcag cggcagcagc ttcaccttgg
     5701 cgtacacgct cgtctcggcg gtgttcgccg tcgcgtggct cgtcgggctc cgcctgcacg
     5761 gcacccgcga tccgaagacg gtcggcaacg gcacgcagga gtaccgcagc atcgccgatg
     5821 ccagcatccg caccttcggc atcctgacga tcgccgcctt cctgctgcac atcgaggtgg
     5881 cgcgcgcgta tctgctgctc gcgcttccgg tgggactgct cctgctgatc gcgggccgct
     5941 ggatgtggcg cggctggctc agcgcgcagc gctcgcaggg ccgcttcctc acgaggatcg
     6001 tgatcgtcgg cgagcacgcc aagtcagtgc acatcgcgcg ggaggtgcag cgcgaccaca
     6061 ccgcgggcta cgagatcatc ggcgcggtca ccgaacgcgg ccacggactg ctcctcccgg
     6121 gcatccccgt gatcggcgac ctcgacggca tgcttcccgc cctggaggcg gtgggcgcgg
     6181 atgccgtggt ctacagcggg tccgacctga tctcgccccc acggctgcgt cagttcggct
     6241 gggagctgca gacgcgcagc atcgacctga tcgtcgccgc cgcgctgacg gacatcgccg
     6301 ggccccgcat ccacgcccgc ccggtcgcgg gactctcact catccacgtg gactatccgg
     6361 agttcaccgg catgcggtac gccaccaagc gcgcgttcga cgtgtgcgtc tcagcgctcg
     6421 cgctgctggt gctctcgccg ctgctgctgg cgatcgcctt cgcggtgcgc acagacggcg
     6481 ggcccgccct gttcgttcag gagcgcatcg ggctggacgg ccggcgcttc cggctgctga
     6541 agttccggtc gatggtgccg gatgccgagg acatgctctc caacctgctg gcgttctcgg
     6601 acggcaacgg cgtgctgttc aagatgcgtg acgacccgcg ggtgaccagg gtgggcggcg
     6661 ttctgcggcg gttcagcctg gatgagctgc cgcagctgtg gaacgtgctg cgcggcgaca
     6721 tgtcgctgat cggccctcgt ccgccgctga tgcgcgaggt gcagaactat gaggacttcg
     6781 cgcagcgtcg tctgctcgtc aagccgggca tcacgggcct ctggcaggtg agcggtcgct
     6841 cggacctgtc gtgggaggac agcgtccgcc tcgacctcta ctacgtggag aactggtcgc
     6901 tgaccggcga cctcgtcatc ctctggcgca cggtccgcac cctgctgcgc ccccacggcg
     6961 cctactgagc tgctccggta ccgggcgcag cgagggtcag gcggccgggc gggtgccgtg
     7021 cagggactcg tagatgtcgg cgacgaactc ctcgatgtcg ggatgcccga ccaccgggcc
     7081 gcccgcgagc aggccgtccg cccccagcag caccgcgctc ggcgtgcgcg gcatctcgag
     7141 agaatcgcgc acgtacccgt cggggtcgtg cagcgtgaac ggctcggtgg cgctggccat
     7201 cggactcgcc tccggcgtgc tgcgcaccag cagctgcacg gatgcctcgg gcagcagctc
     7261 acggtaggcg tcggcgcgct cgatcaccgg cgtgcacgag ccgcaggtct ccgacaccga
     7321 gagcagcagg atcggcttct ccatcgacag cgcccgcagg ttcatgacgg caccgtcgcc
     7381 acgcgtgacc ggcacagccg gcgtgcgggt gcggaggtac tcccccagct cctcgtcggc
     7441 gatccccgcg ggagcagcgg tcacgacctc gggtgccccc ggctgattca ccagcacgaa
     7501 cgtcacggcg acggcggcga ggcccagagt ccagggccac agccacgtcg ccaccgcggc
     7561 gaccccgccc agtggcggcg tc
//
LOCUS       DATMSX010000386        10508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147583,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000386 DATMSX010000000
VERSION     DATMSX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147583"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VN041_11805"
     CDS             <1..553
                     /locus_tag="VN041_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HWU29757.1"
                     /translation="LPARGLHYAADAAAALAVASRVLGDEFSTDAAAQGFSRMAPAYG
                     RGEVIPLRRDPAGEQVEFVMFKNAPSLQMNLDALDGAPERALMAIDEGTPDVSWLYDV
                     DFHALESVDVVTGEKAYQLALALEHAGVRIGTVEPDMEKAVAMMRELPQTAAGRQIWF
                     VNYELMMIGRRLLGHGDQEVARR"
     gene            550..1323
                     /locus_tag="VN041_11810"
     CDS             550..1323
                     /locus_tag="VN041_11810"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWU29758.1"
                     /translation="MTATVRIAQLYPEILGITGDRGNVRALQVRLERAGATVDVLPVG
                     IGEELPADLDILVIGNGPLSAMRGVHADLVARGDGIRSHVADGRALLAIGGGAELLSN
                     GVTTLEGETVEGLGIFDATVSRTKKRKVGYIVVETAVGRVIGFEDHASEWTLSDAAHS
                     YGRVAFGQGGFALPGDGDQAARGEIIRVVNAIAANIQGPMLPLNPELVREIITGVADR
                     RGLELDLTIGVESLDTYAAGARAAIETLVHGKGFNSIQL"
     gene            1338..2033
                     /locus_tag="VN041_11815"
     CDS             1338..2033
                     /locus_tag="VN041_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HWU29759.1"
                     /translation="MSGSAAPRVLLSRSALMQNTQGLLASSDGVVDLRHDAYGHGLDE
                     VAPIVLEAGASAVRVGSAQEARRLEAAGIPATTDALPEVDPHRLLGIPDAEGRLSTQP
                     VMSLQGRVLSTKPLHAGEAVSYGYTHRASRDTRVALVTGGYAQGILRALGNRARVQIG
                     EHTHPIVGRIAMDVCVVDLEGADVPVDAPVTYFGGSGAMRDALAQWSAATGLTALEMA
                     VVIGRNGERSWQA"
     gene            2030..3064
                     /locus_tag="VN041_11820"
     CDS             2030..3064
                     /locus_tag="VN041_11820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWU29760.1"
                     /translation="MSTPVLEISRAAFRANIAAVLERLDGSQLMLVMKDDAYGHGIEW
                     AVAEAEASGVSWFGGYDIPTALRIRAVTTQRVYAWAPSTSPEVEAAVGAGIELGVGSM
                     EYLHEVIAVAERLGARLAVHLKIDTGLHRNGFRPEVWADAVAVARDAEDRGSIRIVGV
                     WSHLAEASDEEDDAAQAVFLNAVRELQNAGGEPEVLHLTASAAAWWRPELRGTLSRIG
                     AFCYGIRSAEGPQLEGLSLISRLVATVDAVEGDLVRIGVGAFDGLPSILRGAEVVTPG
                     GLRAISRIDGDSTLVHGWPGAQAGDRIVVFGPGADGESDATALAERIDTVGEEILTRL
                     TPTVRRELVD"
     gene            complement(3068..3277)
                     /locus_tag="VN041_11825"
     CDS             complement(3068..3277)
                     /locus_tag="VN041_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29761.1"
                     /translation="MNATTERETVQYRLTAMDRCDSCGAQAYIAAEVNGTEMLFCAHH
                     GRKYEEKLRAVATSWHDETARLIEA"
     gene            3459..5567
                     /locus_tag="VN041_11830"
     CDS             3459..5567
                     /locus_tag="VN041_11830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HWU29762.1"
                     /translation="MTNEYSAHHLQVLEGLEAVRKRPGMYIGSNGSPGLMHCLWEIID
                     NSVDEAVAGNGSRIDIVLHADGSVEVADRGRGVPVDVEPRTGLTGVEVVYTKLHAGGK
                     FGGGSYAASGGLHGVGASVVNALSERLDVEVDRGGKTYAMSFHRGEPGVFADTGEPRP
                     DSPFTPFENKSELRVIGKVAKGVTGTRVRYWADRQIFTKDAAFQLGDLENRARQTAFL
                     VPGLELVVRDERPSSSPDDEAGARVTSYLYEGGISEFVDYLASDAPVTDTWRIQGSGS
                     FTETVPVLQADGHMKATEVERECQVDIALRWGTGYETVSRSFVNIIATPKGGTHQQGF
                     EQELLKSLRGQVEQNARRLKVGNDKLEKDDVLAGLTAVLTVTVPEPQFEGQTKEVLGT
                     PAVRSIVAKVVRTELAARFGSTKRDDKSQTSQLLDKIVSEMKARVSARAHKETQRRKT
                     ALESSSLPAKLVDCRSNDVEQSELFIVEGDSALGTARHARNSEFQALLPIRGKILNVQ
                     KASISDMLGNAECAAIITTIGAGSGRTFDLSQARYGKVILMSDADVDGAHIRTLLLTL
                     FFRYMRPLVEAGRVFSAVPPLHRVIVMNPGSKPNETIYTYSEAELHALLAKLQKAGKR
                     WQDPIQRYKGLGEMDADQLATTTMDKQGRLLRRVTIEDAEAADAAIRTFELLMGSEVA
                     PRREFIMSSSDRLSRESIDA"
     gene            5564..6001
                     /locus_tag="VN041_11835"
     CDS             5564..6001
                     /locus_tag="VN041_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29763.1"
                     /translation="MMAPGVRLRAATADDLEHVVSLKERVLRADLERLIGWDPAKSRA
                     RVVEHFSPQHTRMILLEDALVGTITLRPDRDVVWLEMFYLDATLHGRGIGTSVLASVL
                     EAATTPLRLQVLVGSPAQRLYARHGFVVEEDDGIDVWMRRPAP"
     gene            complement(6035..8473)
                     /locus_tag="VN041_11840"
     CDS             complement(6035..8473)
                     /locus_tag="VN041_11840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HWU29764.1"
                     /translation="MPKPSSSEPVQERIQDIDLSQEMQGSFLEYAYSVIYSRALPDAR
                     DGLKPVQRRILYQMAEMGLRPDRGHVKSARVVGEVMGKLHPHGDSAIYDALVRLAQDF
                     ALRVPLVDGHGNFGSLDDGPAASRYTEARLAASALALTENLDEDVVDFVPNYDGQFQQ
                     PAVLSAAFPNLLVNGASGIAVGMATNMAPHNLIEVIAAATHLLENPDATTAELMEFVP
                     GPDFPSGGILMGLDGVKEAYETGRGAFKLRAKTSIEPLGPRRTGIVVTELPYQVGPER
                     VIEKLKDAVNAKKLQGISDVQDLTDRKNGLRLVVTVKTGFDPAAVLDQLFRFTPLEDS
                     FSINNVALVDGQPRTLGLKELLRVYLQHRIEVIERRSRYRLARRNERLHLVRGLLLAI
                     LDIDEVIQVIRSSDDSETARARLQQVFELDEVQAEYILELRLRRLTKFSRLELETERD
                     KLLAEIAELEKLLADPGLVRAQVALELDAVAEQFGTHRRTLLLNAAPPKARTTKSAAE
                     LQLADAPTTLLLSTTGRAVRVDLEPGATIATPARRSKHDAIQARLETTTRSEIGAVTS
                     AGRVVRFTPIDIPAVPASSVGLGAGVRIHDYLGLTDRSERVLALIRFDDDTPLALGTK
                     DGVVKRVVPSGLAVRPELEIIGLKPKDVVIGAAPASDETELVFVSSDAQLLHFPASNV
                     RPQGAPAGGMAGVKLGSGAEVISFTVVASPEDAVVITVSGSEGTLAGADAGRAKVSDF
                     ADFPGKGRATGGVRAHAFLKGEDRLRLAWVGVEPRAIGSDGAARQLPASGAKRDASGQ
                     PLDSVIGSVGTAIG"
     gene            8559..9680
                     /locus_tag="VN041_11845"
     CDS             8559..9680
                     /locus_tag="VN041_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWU29765.1"
                     /translation="MPLMLPSSLESTRSIAGVAPQLLAALRDAPGELPPVRSAVLVVV
                     DGLGAVPLRAHAGHARTLVSAMAKKDVAGSVFPTTTAAALTSLLTGSAPGVHGMVGYR
                     VRHPERDVLVNQLTEWDGVDPTAWQPQATVFEQAAAAGHPTFAVGIAAHAGTGLTHAI
                     LRGAEFRSAGSPAERVELAWELADAHDGALVYCYLPEADKAGHKHGVDSAEWVAALED
                     IDAALSRRVPDGVGVLVTADHGMVDVPAHRQLILDAAGGWHDGIRHIGGEPRMLHVYI
                     EPDADQPGILSRWRRDLDGFADVVSRVEAIDAGLFGGMVTDAARERVGDLLVIARSNH
                     ALYDGEAADQRGRGMVGQHGALTMEEWRVPLIRMGAFSR"
     gene            complement(9704..>10508)
                     /gene="sepH"
                     /locus_tag="VN041_11850"
     CDS             complement(9704..>10508)
                     /gene="sepH"
                     /locus_tag="VN041_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HWU29766.1"
                     /translation="GVSEADVARFEGPVLAEREHIVGQALAVPVLIGSEVEPDAQPTF
                     GVAVRAKLADLQASDERWTSWKDESGWTIKLEFTADEVEHDARWAFDPRRSTLSPLNP
                     DATQLSRQGALPEGLIPRLRAVDQERVSPYKDDSRFDSGAFGPRLLPRPEEETADASA
                     TEHSSAAVQAAAARRAPEESHPNAETADLLEALRRRRGQRESAPLFGEDEHDEPAPIA
                     LFDAIEDEAPAEPEPAPEPEPSEQPSGRRKRRNAMPSWDEIVFGARTEE"
BASE COUNT         1548 a         3574 c         3810 g         1576 t
ORIGIN      
        1 cctgcccgcg cgcggtctgc actacgccgc cgacgccgcc gccgcactgg cagtggcctc
       61 gcgcgtgctc ggcgatgagt tctccacgga tgccgccgcg cagggcttct cgcgcatggc
      121 gcccgcatac gggcgaggcg aggtcatccc gctgcgtcgc gaccccgcgg gggagcaggt
      181 cgagttcgtg atgttcaaga acgcgccgag cctgcagatg aacctcgacg cgctggacgg
      241 ggcgcccgag cgcgccctga tggcgatcga cgagggcacg cccgacgtct cgtggctgta
      301 cgacgtggac ttccacgcgc tggagtccgt cgacgtcgtc acgggcgaga aggcctacca
      361 gctcgcgctg gccctggagc acgccggcgt ccgcatcggc accgtcgaac ccgacatgga
      421 gaaggccgtc gcgatgatgc gggagctgcc gcagaccgcc gccggccgcc agatctggtt
      481 cgtcaactac gagctcatga tgatcggccg caggctcctc ggccatggtg accaggaggt
      541 ggctcgccga tgaccgcgac cgtgcgcatc gcgcagctgt accccgagat cctgggcatc
      601 accggcgacc ggggcaatgt ccgcgccctg caggtgcgcc tcgagcgcgc cggcgcgacc
      661 gtcgacgtgc tgcccgtcgg catcggggag gagctgcccg ccgacctcga catcctcgtg
      721 atcggcaacg gcccgctgtc cgccatgcgc ggcgtgcacg ccgatctggt ggcccgtggc
      781 gacggcatcc gctcccatgt ggccgacgga cgtgcgctgc tcgccatcgg cggcggcgcc
      841 gagctgctca gcaacggcgt cacgacgcta gagggcgaga cggtcgaggg cctcggcatc
      901 ttcgacgcca ccgtgtcgcg caccaagaag cgcaaggtgg gctacatcgt cgtcgagacc
      961 gcggtcggcc gcgtgatcgg gttcgaggat cacgccagcg agtggactct ctccgacgcg
     1021 gcgcacagtt acggtcgcgt ggcgttcggt cagggcggtt tcgctcttcc gggcgacggg
     1081 gaccaggctg cgcgcggcga gatcatccgc gtcgtcaacg cgatcgccgc gaacatccag
     1141 gggccgatgc tcccgctgaa tcccgagctg gtgcgcgaga tcatcaccgg cgtcgccgat
     1201 cggcggggcc tggagctcga tctgacgatc ggcgtcgagt cgctggacac gtacgcggcc
     1261 ggtgctcgcg cggccatcga gaccctcgtg cacggcaagg gcttcaactc catccagctc
     1321 tgagtcggag gcgacagatg tcaggcagtg cggcgccccg ggtcctgctg agccgttccg
     1381 cgctgatgca gaacacgcag ggactgctcg cgtcttcgga cggggtcgtc gatctgcggc
     1441 atgacgccta cggccacggc ctggacgagg tcgccccgat cgtcctggaa gccggcgcat
     1501 ccgcggtgcg ggtcggctcc gcccaggagg cccgtcggct ggaggcagcc ggcatccccg
     1561 cgaccaccga cgccctcccg gaggtcgacc cgcaccggct gctcggcatc ccggatgccg
     1621 agggcaggct ctccacgcag cccgtgatga gcctgcaggg ccgcgtgctc tcgaccaagc
     1681 ccctgcacgc cggggaggcg gtctcgtacg gctacaccca ccgcgcgagc cgggacaccc
     1741 gggttgccct ggtgaccggc ggctacgctc agggcatcct gcgcgcgctc ggcaatcggg
     1801 cccgcgtgca gatcggtgag cacacgcatc cgatcgtcgg tcgcatcgcg atggacgtct
     1861 gcgtggtcga cctggagggg gccgatgtcc cggtcgacgc tcccgtgacc tatttcggcg
     1921 gcagcggtgc gatgcgcgac gcgctcgcgc agtggtctgc ggccacgggc ctgaccgcgc
     1981 tggagatggc ggtcgtgatc ggacgcaacg gcgaacggag ctggcaggca tgagcacccc
     2041 ggtcctggag atctcccgag ccgcattccg cgcgaacatc gccgcggtgc tcgagcggct
     2101 ggacgggtcg cagctgatgc tggtgatgaa ggacgacgcc tacgggcacg gcatcgaatg
     2161 ggccgtcgcc gaggcggaag ccagtggcgt ctcctggttc ggaggctacg acatccccac
     2221 ggcgctgcgc atccgcgccg tcaccacgca gcgggtgtac gcgtgggcgc cgtcgacgag
     2281 tcccgaggtc gaggccgcgg tcggcgcggg catcgagctg ggcgtcggct ccatggagta
     2341 cctgcacgag gtgatcgcag tggcagagcg cctcggcgca cgcctcgcgg tgcacctgaa
     2401 gatcgacacg ggtctgcatc gcaacggctt ccgtccggag gtgtgggcgg atgccgtcgc
     2461 cgtcgcgcgc gatgcggagg atcgaggcag catccggatc gtcggggtct ggagtcatct
     2521 cgccgaggcc agcgatgagg aggacgacgc cgcgcaggcg gtgttcctga acgccgtgcg
     2581 cgagttgcag aacgccggtg gggagccgga ggtgctgcac ctgacagcat ccgccgccgc
     2641 ctggtggcgc cccgagctgc gggggaccct gagccgtatc ggtgcgttct gctacggcat
     2701 ccgctcagcc gaggggcccc agctggaggg cctgtccctc atctcgcgcc tggtggccac
     2761 cgtggacgcc gtggaaggcg acctggtgcg gatcggcgtc ggtgcgttcg acggcctgcc
     2821 gtcgattctc cgtggcgcgg aggtggtgac cccgggagga ctgcgggcga tctcccggat
     2881 cgacggcgac agcacgcttg tgcacggctg gccgggtgcc caggcaggcg atcgcatcgt
     2941 cgtgttcgga cccggtgccg atggggagtc ggatgccacg gctctcgccg agcggatcga
     3001 cacggtgggc gaggagatcc tcacccggct cacgccgacg gtgcggcgtg aactcgtgga
     3061 ttgaggctca ggcctcgatg aggcgggcgg tctcgtcgtg ccagctggtg gcgaccgcgc
     3121 gcagcttctc ctcgtacttg cggccgtggt gggcgcagaa gagcatctcc gtgccgttga
     3181 cctcggctgc gatgtaggcc tgcgctccac aggagtcgca ccggtccatc gccgtgaggc
     3241 ggtactggac ggtctctcgc tcagtcgtag cgttcatgtc ggtgcctcct cgggattcgg
     3301 gctcgattcg agatcgtgct caatacaacc acgggtcacc tgagtgcatg cccggatcac
     3361 ggcgtgtttc gctgtgcgcg tacgcgccgc gtaacggctg ggtcgcgggg gagtgtcgcg
     3421 gcgtccgcgg cggcggccgc gaaatagaat cgaacattgt gaccaacgag tattccgccc
     3481 atcatctcca ggtgctcgag gggctcgaag cggttcgcaa gcgcccgggc atgtacatcg
     3541 gatccaacgg atcaccgggc ctcatgcact gcctctggga gatcatcgac aactccgtcg
     3601 atgaggccgt cgccggtaac gggtcgagga tcgacatcgt gctgcacgcc gacggcagtg
     3661 tcgaggtcgc cgaccgcggg cgaggagtcc cggtcgacgt ggagccgcga acggggctga
     3721 ccggtgtcga ggtcgtctac acgaagctgc acgcgggcgg gaagttcggc ggcggttcct
     3781 atgcggcatc cggtggtctg cacggtgtcg gcgcatcggt cgtgaacgcc ctgtccgagc
     3841 gactcgacgt cgaggtcgac cgcggcggca agacgtacgc catgtcgttc caccgcggcg
     3901 agcccggcgt gttcgccgac acaggggagc cccgcccgga ctcgcccttc acgcccttcg
     3961 agaacaagag cgagctgcgc gtgatcggca aggtcgccaa gggggtcacc ggcacgcgcg
     4021 tcaggtactg ggccgaccgg cagatcttca ccaaggacgc ggcgttccag ctcggcgacc
     4081 tcgagaaccg tgcccgccag accgcgttcc tcgtgcccgg cctcgaactg gtcgtgcgcg
     4141 atgagcgtcc ttcgtcgtcc ccggacgacg aagccggtgc cagggtcacg tcgtacctgt
     4201 acgagggcgg catctccgag ttcgtcgact acctggcctc cgatgctccc gtgaccgaca
     4261 cctggcgcat ccagggcagc ggatccttca ccgagacggt ccccgtgctg caggccgatg
     4321 gccacatgaa ggccaccgag gtggagcgcg agtgccaggt cgacatcgcg ctgcgctggg
     4381 gaaccggcta cgagaccgtc agccgctcgt tcgtgaacat catcgccacg cccaagggcg
     4441 gcacgcatca gcagggcttc gagcaggagc tgctcaagtc gctgcgcggg caggtcgagc
     4501 agaacgcgcg acgcctcaag gtcggcaacg acaagctcga gaaggacgac gtgctcgcgg
     4561 ggctcaccgc cgtgctgacc gtcaccgtgc cggagccgca gttcgagggc cagaccaagg
     4621 aggtgctggg cacccctgcg gtgcgctcca tcgtcgcgaa ggtcgtgcgc acagagctgg
     4681 ccgctcgttt cggatccacc aagcgtgacg acaagagcca gacctcacag ctgctcgaca
     4741 agatcgtctc cgagatgaag gcccgcgtct ccgcgcgtgc acacaaggag acccagcgcc
     4801 gcaagaccgc gctcgagtcg tcgtcgctgc ccgcgaagct ggtggactgc cgctccaacg
     4861 acgtcgagca gagcgaactg ttcatcgtcg agggcgactc ggccctcggc accgctcgtc
     4921 atgcccgcaa cagcgagttc caggcgctgc tgcccatccg cggcaagatc ctcaacgtgc
     4981 agaaggcgtc catcagcgac atgctaggca acgccgagtg cgcggcgatc atcaccacga
     5041 tcggcgcggg ctccggacgc acgttcgatc tgagccaggc gcgctatggc aaggtcatcc
     5101 tgatgagcga cgccgacgtc gacggcgccc acatccgcac gctgctgctg acgctgttct
     5161 tccgctacat gcggccgctg gtggaggcgg gccgggtgtt ctcggccgtg ccgccgctgc
     5221 accgtgtgat cgtgatgaac ccgggatcca agcccaacga gacgatctac acgtactccg
     5281 aggccgagct gcacgcgctg ctggcgaagc tgcagaaggc gggcaagcgc tggcaggacc
     5341 cgatccagcg ctacaagggt ctcggcgaga tggatgccga tcagctggcg accacgacca
     5401 tggacaagca gggtcgtctg cttcgtcgcg tgaccatcga ggatgctgag gcggcggatg
     5461 ccgcgatccg caccttcgaa ctgctgatgg gcagcgaggt ggcaccgcgt cgcgagttca
     5521 tcatgagctc cagtgacagg ctgagccgcg agtccatcga cgcatgatgg cgccgggggt
     5581 gcggctgcgc gctgcgaccg ccgatgacct ggaacacgtc gtctcgctca aggagcgcgt
     5641 gctccgtgca gacctggagc ggctgatcgg ctgggatccg gcgaagtcga gggcgcgcgt
     5701 cgtcgagcac ttctcaccgc agcacacccg gatgatcctg ctggaggatg ccctggtcgg
     5761 cacgatcacg ctgcgcccgg accgggatgt ggtctggctg gagatgttct acctcgatgc
     5821 gacgctccac ggccgcggca tcgggacgtc cgtcctggcg agcgtgctgg aggctgccac
     5881 caccccgctg cgcctccagg tgctcgtcgg ttcgccggcg cagcggctct acgcccgtca
     5941 cggcttcgtc gtggaggagg acgacggcat cgacgtctgg atgcgccgcc cggccccctg
     6001 agtccgcgat cctgaggcca ggggccggac gcgctcagcc gatcgcggtg ccgacggagc
     6061 cgatcacgga gtcgagaggc tgtccggagg cgtcgcgctt ggcgccggac gccggcagct
     6121 gacgcgcggc accgtccgat ccgatcgcac ggggctccac gcccacccag gccagacgca
     6181 ggcgatcctc gcccttcagg aacgcgtgcg cacgcacacc gccggtggcg cggcccttgc
     6241 ccgggaaatc cgcgaagtcc gacaccttgg cgcgtccggc atccgctccc gccagcgtcc
     6301 cctcggagcc ggagaccgtg atgaccacgg cgtcctccgg ggaggccacg acggtgaacg
     6361 agatgacctc ggcgccggaa ccgagcttca cgcccgccat cccgcccgct ggtgcgccct
     6421 gagggcgcac gttcgacgcg gggaagtgca gcagctgagc atcggagctc acgaagacga
     6481 gctccgtctc gtccgatgcc ggcgccgcgc cgatgacgac atccttcggc ttcaggccga
     6541 tgatctcgag ctcggggcgc acggccaggc ccgacgggac gacccgcttg acgacgccgt
     6601 ccttggtgcc cagcgccaat ggcgtgtcgt cgtcgaaccg gatcagggcg agcacccgct
     6661 cgctccggtc ggtcagcccc agatagtcgt ggatccgcac gccggcgccc agcccgaccg
     6721 agctcgcggg cacggcgggg atgtcgatgg gcgtgaaccg cacgacccgg cccgcactcg
     6781 tgacagcgcc gatctcactg cgcgtggtcg tctccaggcg cgcctggata gcgtcgtgct
     6841 tgctgcggcg ggcgggcgtc gcgatcgtcg cccccggctc caggtccacg cgcacggcac
     6901 gcccggtcgt cgacagcagc agggtggtcg gggcgtccgc gagctgcagc tcggcagccg
     6961 acttggtcgt gcgcgccttg ggcggtgccg cgttcagcag cagggtgcgc cggtgcgtgc
     7021 cgaactgctc ggccacggca tccagctcca gcgcaacctg ggcacggacc agaccggggt
     7081 cggcgagcag cttctcgagt tcggcgatct cggcgagcag cttgtcgcgc tcggtctcca
     7141 gttcgaggcg ggagaacttc gtcaggcggc gcagacgcag ctcgaggatg tactcggcct
     7201 gcacctcgtc cagctcgaac acctgctgaa ggcgggcgcg cgccgtctcg gagtcatccg
     7261 aggagcggat gacctggatg acctcgtcga tgtcgaggat cgccagcagc aggccgcgca
     7321 ccaggtgcag acgctcgttg cggcgggcga gccggtagcg gctgcgccgc tcgatgacct
     7381 cgatgcggtg ctgcaggtac acccgcagca gctccttgag acccagggtg cggggctgcc
     7441 cgtcgaccag cgcgacgttg ttgatcgaga acgagtcctc gagcggcgtg aaccggaaca
     7501 gctggtcgag caccgccgcc gggtcgaacc cggtcttgac cgtcacgacc aggcgcagac
     7561 cgttcttgcg gtcggtgagg tcctgcacgt cgctgatgcc ctgcagcttc ttggcgttga
     7621 cggcgtcctt gagcttctcg atgacgcgct cgggacccac ctggtagggc agctcggtga
     7681 cgacgatgcc ggttcggcgc gggccgagcg gctcgatcga ggtcttggcg cgcagcttga
     7741 acgcgccgcg gccggtctcg taggcctcct tgacgccgtc caggcccatg aggatgccgc
     7801 cggaggggaa gtccgggccc ggcacgaact ccatcagctc ggccgtggtc gcatccgggt
     7861 tctcgagcag gtgcgtggcg gcggcgatca cctcgatgag gttgtgcgga gccatgttcg
     7921 tggccatgcc caccgcgatg ccgctcgcgc cgttgacgag caggttgggg aaggccgccg
     7981 acagcaccgc gggctgctgg aactggccgt cgtagttggg gacgaagtcg acgacgtcct
     8041 cgtcgaggtt ctcggtcaga gccagagcgg atgccgcgag ccgggcctcc gtgtagcgcg
     8101 atgcggcggg accgtcatcg agggagccga agttgccgtg cccgtcgacc agcggcacgc
     8161 gcagggcgaa gtcctgcgcg aggcgcacca gggcgtcgta gatggcggag tcgccgtgcg
     8221 ggtgcagctt tcccatcacc tcgccgacga cgcgggcgct cttgacatgg ccgcggtcgg
     8281 gccgcaggcc catctcggcc atctggtaca ggatgcggcg ctgcacgggc ttgaggccgt
     8341 cgcgggcgtc cggcagggcg cgcgagtaga tgaccgagta cgcgtactcg aggaacgagc
     8401 cctgcatctc ctgggagagg tcgatgtcct ggatgcgctc ctggacgggc tcggatgacg
     8461 agggctttgg catggacttc ctgctggacg ctcgggtgcg cacgggtgac tgggcacgag
     8521 tgcggggcgg gcgggccgcc tgtggaagac tggcccggat gcctctcatg ctaccgtcca
     8581 gcctcgaatc gacgcggagc atcgccgggg tggcgcccca gctgctcgcc gccctgcgcg
     8641 acgctccggg ggagctgccg cctgtgcgat ccgccgtgct cgtcgtcgtg gacggactcg
     8701 gcgccgtgcc gctgcgcgcg catgccgggc acgcccgcac cctggtgtcg gcgatggcga
     8761 agaaggacgt cgcgggctcg gtgttcccga cgacgacggc cgccgcactg accagcctgc
     8821 tgaccgggtc cgctccggga gtgcacggga tggtcggcta ccgggtgcgg catccggagc
     8881 gcgacgtgct cgtcaaccag ctcacggaat gggacggcgt ggacccgacc gcctggcagc
     8941 cgcaggcgac ggtgttcgag caggcggctg ccgccgggca tccgaccttc gctgtgggca
     9001 tcgccgcgca cgcgggcaca ggactgaccc acgcgatcct gcgcggcgcc gagttccgca
     9061 gcgccgggtc accggcagaa cgcgtggagc tggcttggga gctcgccgac gcgcacgacg
     9121 gcgccctggt gtactgctat cttccggaag cggacaaggc cgggcacaag cacggcgtcg
     9181 actccgccga atgggtcgcc gccctcgagg acatcgacgc ggcgctgtcg cgacgggtgc
     9241 cggacggcgt gggagtgctg gtcacggccg accacggcat ggtcgacgtg cccgcgcacc
     9301 ggcagctgat cctggacgcc gcggggggct ggcacgacgg catccggcac atcggcgggg
     9361 aaccgcggat gctgcacgtg tacatcgagc cggatgccga ccagccgggc atcctgagcc
     9421 gctggcgtcg cgacctggac ggattcgccg atgtggtcag ccgcgtcgag gcgatcgacg
     9481 cagggctctt cggcgggatg gtgacggatg ccgcgcgcga gcgcgtcggc gacctgctcg
     9541 tgatcgcgcg ctcgaatcac gcgctctacg acggcgaggc ggcggatcag cgcggtcggg
     9601 gcatggtcgg ccagcacggc gcgctgacca tggaggagtg gcgcgtcccg ctcatccgga
     9661 tgggcgcctt ctcgcgctga tcagactgcg ggctcggtga ccgtcactcc tcggtgcggg
     9721 ccccgaagac gatctcgtcc cagctcggca tcgcgttgcg gcgcttgcgc cgcccggacg
     9781 gctgctcgga cggctcgggc tccggagccg gttccggctc ggcgggcgcc tcgtcctcga
     9841 tggcgtcgaa cagcgcgatg ggcgccggct catcgtgctc gtcctcgccg aacaggggag
     9901 cggactcgcg ctgcccgcgg cggcgtcgca gcgcctccag caggtcggcg gtctccgcgt
     9961 tggggtggct ctcctccggc gcgcggcgcg cggcggccgc ctgcacagcg gcgctggagt
    10021 gctccgtggc ggatgcatcc gcggtctcct cctccggacg ggggagcaga cggggaccga
    10081 acgcgccgga gtcgaagcgg ctgtcgtcct tgtagggcga gacgcgctcc tggtcgaccg
    10141 cgcgaagacg cgggatcagc ccctcgggaa gagcgccctg gcgggagagc tgcgtcgcat
    10201 ccgggttcag gggcgagagc gtgctgcgac gcggatcgaa tgcccagcgt gcgtcgtgct
    10261 cgacctcgtc ggcggtgaac tccagcttga tggtccagcc ggactcgtcc ttccagctcg
    10321 tccagcgttc gtcgctcgcc tgcagatcgg cgagcttggc acgcacggcg acgccgaagg
    10381 tcggctgcgc gtcgggctcg acctcgctgc cgatcagcac cggaacggcg agggcctggc
    10441 cgacgatgtg ctcgcgctcg gcgagcacag gaccctcgaa gcgcgcgacg tccgcctcgc
    10501 tgacgccg
//
LOCUS       DATMSX010000387         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_166856,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000387 DATMSX010000000
VERSION     DATMSX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166856"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VN041_11855"
     CDS             <1..342
                     /locus_tag="VN041_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HWU29767.1"
                     /translation="VADRLRKMTEEPEPLRALVEDARVAAWGDVRGNLDRRLRVEAMR
                     PDQDPDYERMRDARMQALKLVDLQALSSEQRARRKREKAAEKAAEKEAAKEVARSERS
                     ARREARHAGDA"
     gene            412..484
                     /locus_tag="VN041_11860"
     tRNA            412..484
                     /locus_tag="VN041_11860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Arg,seq:acg)
     gene            complement(516..1466)
                     /locus_tag="VN041_11865"
     CDS             complement(516..1466)
                     /locus_tag="VN041_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU29768.1"
                     /translation="MNLEQLRGFVEVARLGHFTRASEVLHVAQPSLSRQISTLEHELG
                     GRLLHRARGHITLTPAGEVLLPRAQRILAEVGAIRAEMDELAGLRRGRVRLGAPPTLC
                     VSLVAEAISTFHGAHPGVDLHLAEAGSRALVEQLAIGELDIALITASDLRPVPGVTLS
                     RLPLLSEELVVVTSSADPLVPSGSTVDLASLSRIPLIALDRSYDLRATTDAAFRAAGL
                     TPTSVLEGPEMDAVLRFVERGLGVAVVPAMVLTDQPGLSSARLSVPLTRTVSLAHRSD
                     VIPGVAVAAMRDLIVATAVRFAERDPALSSILPETEETPS"
     gene            1538..3268
                     /locus_tag="VN041_11870"
     CDS             1538..3268
                     /locus_tag="VN041_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWU29769.1"
                     /translation="MSALERQISTTVLVIGTGGSGLRAAIEVAEHGIDVLAVGKRPRQ
                     DAHTSLAAGGINAALGTMDAEDSWQQHAADTIKESYLLADPKTVEIVTQGAERGIRDL
                     ERWGMGFAREDDGRISQRFFGAHTFRRTAFAGDYTGLEIQRTLVRRAEELDVPILDGV
                     YITRILVRDNVVFGAYGFDQADGTRYLIHADAVILAAGGHTRIWRRTSSRRDENTGDS
                     FRLAVDAGARIRDPELVQFHPSGIIEPESAAGTLVSEAARGEGGVLRNGLGERFMAKY
                     DPERMELSTRDRVALAAYTEIKQGRGTKNGGVWLDVSHLPRETIMTRLPRVYQTLLEL
                     QMLDITVDPIEIAPTAHYSMGGVWVRPEDHGTDVDGLYAVGEAASGLHGANRLGGNSL
                     IELLVYGRIVAQAAMAHALGLDAQRRSTDAVARARAEIDDLLASDGAENVRALQRAIR
                     NTMTEHAGVVRDEQGLRAGLAELDAIEARMADVGIHPDIAGFQDLAHAFDLKASAIAA
                     RATLEAALERRETRGCHNRSDFPETDPTLQVNLVWSPATGVSREPIAPIPAEISELMR
                     EVDSTGKLVE"
     gene            complement(3272..>3595)
                     /locus_tag="VN041_11875"
     CDS             complement(3272..>3595)
                     /locus_tag="VN041_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU29770.1"
                     /translation="AQRRAVVDAFLAAARDGDFAGLLAVLHPDVEFHLDQGADGIRIV
                     RGAEKVAASAAAFHRYATGYDFEIVELADGFAVVAIDDGVPVSLLFFTSEGEQIMAME
                     TRELR"
BASE COUNT          588 a         1262 c         1257 g          488 t
ORIGIN      
        1 gtcgccgacc ggctgcgcaa gatgaccgag gagcccgaac cgctgcgggc tctcgtcgag
       61 gatgcgcggg tcgccgcctg gggtgacgtg cgcggcaacc tcgaccgccg gctgcgcgtc
      121 gaggccatgc gccccgacca ggatccggac tacgagcgga tgcgggatgc acgcatgcag
      181 gccctcaagc tcgtcgacct gcaggcgctc tcgtccgagc agcgcgcgcg ccggaagcgg
      241 gagaaggccg ccgagaaagc ggccgagaag gaagccgcga aagaggtcgc acgaagcgag
      301 cgcagcgctc gccgcgaggc gcgacacgcg ggggatgcgt gacggaccga ggcgatttcg
      361 cttcgctctg atcgttgtgt aagctgttcc ttggcccgct cgccgggcct tgcgcctcta
      421 gctcaatgga tagagcatct gactacggat cagaaggttg ggggttcgag tccctcgagg
      481 cgcacactgt gttgagacag tgctgaaacc ccctctcagg agggggtttc ttctgtctcc
      541 gggaggatgc tgctcagcgc cgggtcgcgc tcggcgaacc gcaccgcggt cgcgacgatc
      601 aggtcgcgca tggccgcgac ggcgacgccg ggaatgacgt ccgagcggtg cgccaggctc
      661 acagtgcgcg tcagcggcac ggacagccgc gccgagctca gccccggctg atccgtgagc
      721 accatcgccg gcaccacggc cacgcccagc ccgcgttcga cgaaccgcag cacggcatcc
      781 atctccggcc cctccagcac ggacgtcggc gtgagcccgg cagcgcggaa cgcggcatcc
      841 gtggtggcgc ggaggtcgta gctgcgatcc agcgcgatca gcgggatccg ggagaggctg
      901 gcgaggtcca ctgtggaacc tgaggggacg agcggatccg cggacgaggt gaccacgacc
      961 agttcctcgg acagcagcgg cagccggctc agcgtcacgc cgggcacggg tcgcagatcg
     1021 gatgccgtga tcagcgcgat gtcgagctcg ccgatcgcga gctgctccac gagtgcccgc
     1081 gatccggcct cggcgaggtg caggtccacc cccggatgcg ctccgtggaa cgtgctgatg
     1141 gcctcggcca ccaggctcac gcagagcgtc ggcggcgcac ccagacgcac ccgtccacga
     1201 cggagtccgg cgagctcgtc catctcggcc cggatcgcgc cgacctcggc caggatccgc
     1261 tgcgcgcgcg gcagcagcac ctcgccggcc ggggtcagcg tgatgtgccc tcgcgcgcga
     1321 tggagcagcc gcccgccgag ctcgtgctcc agagtggaga tctgccggct gagcgacggc
     1381 tgggccacgt gcagcacctc ggatgctcgg gtgaagtgcc ccaggcgtgc cacctccacg
     1441 aatccgcgca gctgttccag gttcatgcgc tcaggctatc gaatacagct gaacaatgca
     1501 ttggagttat cgaagagacg agcctagcgt gaaccacatg agtgctctcg aacgacagat
     1561 ctccaccacc gtcctggtca tcggcaccgg cggatccggg ctgcgcgcgg cgatcgaggt
     1621 cgccgaacac ggcatcgacg tgctggcggt cggcaaacgt ccgcgccagg acgcccacac
     1681 ctccctcgcc gcgggcggca tcaacgcggc cctcgggacg atggatgccg aggacagctg
     1741 gcagcagcac gctgccgaca ccatcaagga gagctacctg ctcgccgacc cgaagacggt
     1801 cgagatcgtc acacagggcg ccgagcgcgg catccgcgac ctcgagcgct ggggcatggg
     1861 cttcgcacgg gaggacgacg ggcgcatctc gcagcggttc ttcggagcgc acaccttccg
     1921 gcgcacggcc ttcgccggcg actacaccgg gctcgagatc cagcgcaccc tggtgcgccg
     1981 tgccgaggag ctggacgtgc cgatcctgga cggcgtgtac atcacccgca tcctggtgcg
     2041 cgacaacgtc gtgttcggcg cctacggctt cgaccaggcc gacggcacac ggtacctcat
     2101 ccacgcggat gcggtgatcc tcgccgccgg gggtcacacc cggatctggc ggcgcacctc
     2161 ctcgcgccgc gacgagaaca ccggcgactc gttccggctc gccgtcgacg cgggcgcccg
     2221 catccgggac cccgaactcg tgcagttcca cccctccggc atcatcgagc cggagagcgc
     2281 cgccggcacg ctcgtgtccg aggcggctcg tggtgaaggc ggcgtcctgc gcaacggcct
     2341 gggcgagcgc ttcatggcga agtacgaccc ggagcggatg gaactgtcca cccgtgaccg
     2401 cgtcgcgctc gcggcgtaca cggagatcaa gcagggccgc ggcacgaaga acggcggcgt
     2461 ctggctcgac gtctcgcacc tgccgcgcga gacgatcatg acccggctgc cgcgcgtcta
     2521 ccagacgctg ctcgagctgc agatgctcga catcaccgtc gatccgatcg agatcgcccc
     2581 gaccgcgcac tattcgatgg gcggtgtctg ggtgcggccc gaggatcacg gcaccgacgt
     2641 cgacggcctg tacgccgtgg gcgaggcggc gagcggtctg cacggtgcga accggctcgg
     2701 cggcaactcg ctgatcgagc tgctcgtcta cgggcggatc gtcgctcagg ctgcgatggc
     2761 gcacgcgctg ggcctggatg cccagcgacg ctcgacggat gccgtcgctc gggcgcgcgc
     2821 cgagatcgac gacctgctcg cctccgacgg cgctgagaac gtgcgggcgc tgcagcgcgc
     2881 catccgcaac accatgaccg agcacgccgg tgtggtgcgt gacgaacagg gcctgcgcgc
     2941 aggactggcc gagctcgacg cgatcgaggc gcggatggcg gacgtcggca tccacccgga
     3001 catcgcggga ttccaggatc tggcgcacgc cttcgatctg aaggcatccg cgatcgccgc
     3061 gcgcgccacc ctggaggcgg cgctggagcg tcgtgagacc cgcggatgcc acaatcgcag
     3121 cgacttcccg gagaccgatc cgaccctgca ggtgaacctg gtgtggtcgc cggccacggg
     3181 cgtctcccgc gagccgatcg cgcccatccc ggccgagatc tccgagctca tgcgcgaggt
     3241 cgacagcacc ggcaagctcg tggagtagcc ctcagcgcag ttcgcgcgtc tccatcgcca
     3301 tgatctgctc gccctccgag gtgaagaaga gcagcgagac ggggacgccg tcgtcgatcg
     3361 cgacgacggc gaacccgtcg gccagctcca cgatctcgaa gtcgtagccc gtcgcgtacc
     3421 ggtggaatgc ggcggcggag gccgccacct tctccgctcc gcggacgatc cggatgccgt
     3481 cggcaccctg atcgaggtgg aactcgacgt cggggtgcag cacggcgagc agccctgcga
     3541 agtcgccgtc gcgcgccgcc gccaggaagg cgtcgacgac ggcccgtcgc tgcgc
//
LOCUS       DATMSX010000388         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_110325,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000388 DATMSX010000000
VERSION     DATMSX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110325"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VN041_11880"
     CDS             complement(<1..743)
                     /locus_tag="VN041_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HWU29771.1"
                     /translation="MSLPPLVEPVDVLSSREQLRTARHQRLAALGSDGQRRIAAASVG
                     VIGAGGLGAPVVLALAAAGVGTIVVFDDDDVELSNLQRQVIHRLQDVGRPKTDSVVRV
                     AADLSETEVRVVTTRLTHENAAELISGVDLVLDGTDSFESREVVAAACERLAVPLVWG
                     TVQEFAGLVTVFWSAPPRPAEAVRLHDLYPPGSEAPSCSAVGVFGPLCIQAGSMMASE
                     ALKLITGIGRPLLGRIAMIDALAGTVREAP"
     gene            840..1232
                     /locus_tag="VN041_11885"
     CDS             840..1232
                     /locus_tag="VN041_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWU29772.1"
                     /translation="MSASYKIALAADLLGVSDDTVRRWVDQGVLPVTDASPAEIPGEA
                     LARHAIALAKSAEDATGVRSSARNRFTGLVTNVVMDTVAAQVEIQAGPHRVVSLMTAE
                     AVRELGLEVGSLAVAVVKATTVIVETPR"
     gene            1260..2111
                     /gene="modA"
                     /locus_tag="VN041_11890"
     CDS             1260..2111
                     /gene="modA"
                     /locus_tag="VN041_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU29773.1"
                     /translation="MRRIPIIAVATAAIVLLLGGCASTASLDTTPTSSRTGGSDASGG
                     GVAPRDAPSPADADVLTGELSVSAAASLKGAFDQAIAQFEKLFPGVHVTATYDGSSTL
                     ATQIDGGAKVDVFASADEANMARVTGAGLASGPTVFARNTLVVVTPKSNPAGIKTLAD
                     LAKPGVKVVLCAPEVPCGAASKKLLAAQRVTLTPVSQEQNVTAVLTKVRSDEADAGLV
                     YKTDAAAATADVRSFTPTGADQVVNAYPIVALKDAPNPDAATTFVSFITGTQGQKILA
                     SFGFDGP"
     gene            2111..>2382
                     /locus_tag="VN041_11895"
     CDS             2111..>2382
                     /locus_tag="VN041_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWU29774.1"
                     /translation="MPRSGSQTTGARGHAPRVLIVPAALGIVFLVLPLAALVGRVQWG
                     TLWPDVTSAEALSALRLSLVTGLVATAICLLIGIPLALTIARAAQRT"
BASE COUNT          350 a          880 c          825 g          327 t
ORIGIN      
        1 ggcgcctcgc gcaccgtgcc cgcgagcgcg tcgatcatcg cgatccggcc cagcaggggc
       61 cgcccgatcc cggtgatcag cttgagcgcc tcggatgcca tcatcgagcc cgcctggatg
      121 cacagcgggc cgaagacgcc cacggccgag cacgacggcg cctcactgcc gggcgggtac
      181 aggtcatgca gtcgcacggc ctccgcaggc ctgggaggcg ccgaccagaa caccgtcacc
      241 aacccggcga actcctgcac ggtgccccac acgagcggca cggccagccg ctcgcaggcg
      301 gcggccacga cctcgcggct ctcgaaggag tccgtgccgt cgagcaccag gtcgaccccg
      361 gagatcagct cggcggcgtt ctcgtgcgtg agccgggtgg tcaccacccg gacctcggtc
      421 tcggacaggt ctgcggccac acgcaccacg ctgtcggtct tgggtcgccc gacatcctgc
      481 aggcgatgga tgacctggcg ctgcaggttg gagagctcga catcgtcgtc gtcgaacacc
      541 acgatggtgc ccacacccgc ggcggccagc gcgagcacga ccggggcgcc caggccgccc
      601 gcgccgatga ctcccacgct ggctgcggcg atgcggcgct gcccgtccga cccgagcgcg
      661 gcgaggcgct gatgccgcgc cgtgcgcagc tgctccctgc tgctgagcac gtcgacgggt
      721 tcgaccagcg gggggaggga catgacctcc agggtagacc tcgccgcggc ggggatggac
      781 tccgcttctg cgggcaagag tcctgctttc gtccgcacat gcggtgatag tctgcggaca
      841 tgtccgcaag ctacaagatc gcgctcgccg cggacctgct cggggtcagt gacgacacgg
      901 tgcgccgctg ggtcgaccag ggggtgctgc cggtcaccga cgccagcccg gccgagatcc
      961 ccggcgaagc gctcgcccgg cacgcgatcg ccctggcgaa gtcggcggag gacgctaccg
     1021 gagtgcgctc cagcgcacgc aaccgcttca ccggcctggt caccaacgtc gtgatggaca
     1081 ccgtcgcagc ccaggtggag atccaggcgg gcccgcaccg ggtggtctcg ctgatgaccg
     1141 ccgaggccgt gcgggagctg ggtctcgagg tcggttcgct cgccgtcgcc gtggtgaagg
     1201 cgacgacggt catcgtcgag accccgcgct gatcgcgcgc gcacgtgagg agaagagcca
     1261 tgcgccgcat cccgatcatc gccgtcgcca ccgcggccat cgtgctgctg ctcgggggct
     1321 gcgcgtccac ggcatccctc gacacgaccc ccacgagcag ccgcacgggg ggatcggatg
     1381 cgtccggagg aggcgtcgca ccgagggacg cgccgtcgcc ggcggatgcc gacgtgctca
     1441 ccggcgagct gtcggtctcg gccgcggcgt ccttgaaggg cgccttcgac caggcgatcg
     1501 cgcagttcga gaagctcttc cccggcgtgc acgtcaccgc gacctacgac ggctccagca
     1561 cgctcgccac ccagatcgac ggcggggcga aggtcgacgt gttcgcctcc gccgacgagg
     1621 cgaacatggc cagggtcacc ggcgccggcc tggcatccgg tccgacggtc ttcgcacgca
     1681 acacgctggt cgtcgtgaca ccgaagtcga acccggccgg aatcaagacg ctcgcggatc
     1741 tcgccaagcc gggcgtcaag gtggtgctct gcgcacccga ggttccctgc ggtgcggcgt
     1801 cgaagaagct gctcgccgcc cagcgggtca ccctcacgcc ggtcagccag gagcagaacg
     1861 tgacggccgt gctcaccaag gtgcgcagcg acgaggcgga cgccgggctc gtctacaaga
     1921 cggatgccgc cgccgcgacc gccgacgtcc gctcgttcac cccgaccggc gccgatcagg
     1981 tcgtgaacgc ctacccgatc gtggcgctga aggacgcccc caaccccgat gctgcgacga
     2041 cgttcgtctc gttcatcacc ggcacgcagg ggcagaagat cctcgcgtcc ttcggcttcg
     2101 acggcccgtg atgcctcgaa gcggctcgca gacgaccggc gcccgcggtc atgcgccgcg
     2161 ggtgctgatc gtgcccgccg cgctcggcat cgtgttcctc gtgctgccgc tcgccgccct
     2221 cgttggccgc gtgcagtggg gaacgctgtg gccggacgtg acctcggccg aggcgctcag
     2281 cgccctgcgg ctgtcgctgg tcacggggct cgtcgccacc gcgatctgcc tgctgatcgg
     2341 catcccgttg gcgctgacca tcgcccgagc cgctcagcgc ac
//
LOCUS       DATMSX010000389        12238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176517,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000389 DATMSX010000000
VERSION     DATMSX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176517"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN041_11900"
     CDS             <1..270
                     /locus_tag="VN041_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HWU29775.1"
                     /translation="MQRRLAALERASGPREPEGRAPMPGSVVALHVADGDTVREGDPL
                     VSIEAMKMEHPVLAPHDGTVHLLVAVGDQVRRDQPVARVSEEETS"
     gene            267..1433
                     /locus_tag="VN041_11905"
     CDS             267..1433
                     /locus_tag="VN041_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWU29776.1"
                     /translation="MTDDLSSIGLNEEQRELAAMVRDFAEQVVAPQAYEADRSHTLNL
                     DVVRQMGELGLFGLPFSEEYGGQGGDYLALGLAIEGLARVDQSIAITLEAGVSLGAMP
                     IYRFGTEEQKQEYLPSLLAAENLAGFGLTEPEAGSDAGATRTTARLEGGEWIIDGSKQ
                     FITNSGTAITRFVTVTAVTGVTDGRKEISTIIVPNGTPGFTVEPAYDKVGWNASDTHP
                     LTFQGVRVPEGNLLGERGRGFKNFLSILDEGRIAIAALATGAAEGCLEAAVDYAQKRT
                     IFGSALSTRQNAQFTLARMRARVHTARLAWHHAARLRDAGLPFAEEAAIAKLVSGDAA
                     MDNARDATQIFGGNGFMNEYPVGRHYRDSKILEIGEGTTEVQLLVIARKLGLAG"
     gene            1460..1936
                     /locus_tag="VN041_11910"
     CDS             1460..1936
                     /locus_tag="VN041_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWU29777.1"
                     /translation="MTDIVQRGLYFEEFELGARYLHRPGRTATEADNVLFTTLTMNTQ
                     ALHLDAAFAGAERPFGQRLMNSMWTLSTMVGSSVAQLTQGTLVAQLGLSDITFPHPVF
                     HGDTLYTESVITDKRLSSSRPGQGIVTIAHTGRNQDGVIVATATRVALMHCLPEGE"
     gene            1936..2739
                     /locus_tag="VN041_11915"
     CDS             1936..2739
                     /locus_tag="VN041_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWU29778.1"
                     /translation="MTFELGPALLFCPADRPERYAGGLAKADAVILDLEDAVLPDAKA
                     QAREHLVAADLDPARVIVRVNDPASAHFTADLEVVARTPFRTVMVAKTEDPSALDAFG
                     EEYALLALCETAAGVLNAGAIAAHPRVAALMWGAEDLVASIGGTASRFPDGRYREVAR
                     HARASVLVAAAAHGKAAIDAVHLDIDDEAGLAAEAADAVASGFRATACIHPKQVAIIR
                     DAYRPDAATLDWARAVLAAADGERGVFRFQGRMVDEPVLRHARAVLSRA"
     gene            complement(2756..3343)
                     /locus_tag="VN041_11920"
     CDS             complement(2756..3343)
                     /locus_tag="VN041_11920"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU29779.1"
                     /translation="MTLLTLVRHGQTDWNLARRIQGSTDIPLNDTGRADALTAAGRLS
                     RDTHHAIYASPLLRARETAEIIAGELGLAAPALVHDVREREFGEGEGLLVQEYIDAYG
                     DWHAVVPGAETLDEVCIRAMSALHRIARDARRRSAPRAESIIVVTHGGVIRSLIDHAS
                     GGTLPKIGSVLGNGSLHRFEVSPGSLRLLQDVHVL"
     gene            complement(3353..4198)
                     /locus_tag="VN041_11925"
     CDS             complement(3353..4198)
                     /locus_tag="VN041_11925"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWU29780.1"
                     /translation="MQFPDTIDDDTRTRIDHVAELLRGRRVAVLTGAGISTDSGIPAY
                     RGVGARTRANPMTIQTYLADESARRRYWLGGHLGWLAFTAPEPNEGHVALARMERAGV
                     VSGVVTQNVDGLHLRAGSSRVVEIHGTMHRTLCLDCGQVFDRRAIADQLEALNPWVRK
                     PENIELNPDGDVTPESTEGFVIPPCTVCGGMLKPEVVFFGEFVPAARFRSAESVVTAA
                     DALLVAGTSLTVNSGIRIIERARRRGIPLVIVNREPTRADAWADAVLAGGTSPVLSAL
                     AGALA"
     gene            4271..5092
                     /locus_tag="VN041_11930"
     CDS             4271..5092
                     /locus_tag="VN041_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWU29781.1"
                     /translation="MHVIPVSDPDDARLDDYRSLTDTALRRRNEPEGGLYMAESAKVI
                     ARAVQAGHRPRSVLTQEKWVDAVADALDGVGSGVETPVYTASAEVSETVTGFAIHRGP
                     LAAMHRPALLSVAEVLQRARPDRARIAVLEGLTDHTNVGAVFRSAAALGVDAVLVTPD
                     CADPLYRRSVRVSMGTVFQVPWTRVPAWPEGMRELQDAGFVVAGMTLGEGAIEVDDLV
                     AEDHPRLAMVLGTEGDGIRPATDALLDRRVTIPMMGGVDSLNVAAASAVAFYATR"
     gene            complement(5141..5971)
                     /locus_tag="VN041_11935"
     CDS             complement(5141..5971)
                     /locus_tag="VN041_11935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU29782.1"
                     /translation="MVDQETSRTPFVENEGPHPWRRFVAVGDSFTEGVGDPAPDLPGG
                     LRGWADRVAEVLGQDVDDFAYANLAVRGKLIAQIVADQIEPAVALKPDLVSICAGGND
                     VIRPGTDPDEIAVQLEDAVARLSSTGAAVVLFTGIDTGFTPVFRPFRGKVAIYNENVR
                     AIGDRYDCIIADQWSLKVVQDPRFFDDDRLHYNALGHHEIARMVLRALNVPNDLQAMQ
                     PDPFPVRTWREARAEDIGWAREHLVPWVLRRLRHQSSGDHVEAKRPEPSPVRMPGAER
                     "
     gene            6083..7822
                     /locus_tag="VN041_11940"
     CDS             6083..7822
                     /locus_tag="VN041_11940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU29783.1"
                     /translation="MLSPSFPQRAPWGTADKLRAWQREALDAYFQADQRDFLVAATPG
                     AGKTTFALTLAVELLRMGEVNRVIVVAPTEHLKTQWADAAARVHIRLDPRFRNSHWAP
                     SRQYHGVVVTYAQVAAKSSVHRHLTEDARTLVILDEVHHGGDALSWGDAIRDAYGPAR
                     RRLLLSGTPFRSDTAPIPFVEYIPDESGARVSSTDYRYGYGRALADGVVRPVLFHMYS
                     GKMRWRTSAGDELEAHLGQDNTKDVTSQAWRTALDPEGEWMPAVLSAADRRLTEIRHH
                     VPDAGGLVLATDQTVARAYAKILHSITGEQPTVVLSDDASASERIEKFSETDRRWMVA
                     VRMVSEGVDVPRLAVGVYATSSSTPLFFAQAIGRFVRARRRGEAASVFLPNVPVLMTL
                     ANEMERQRDHALDRRDKDDDGLDDSLLESANREEDASDALTQEFSYQAISSLAHFDRV
                     VFEGKEFGQLAEPGTPEEEEFIGFPGLLEPEHVHELLMQRQARQGKLRQAREAATPVD
                     TTTLPAPLHRTLREQRQLLNSLVGLYARQTGEPHGSVHAELRRMCGGPAVAQATVSQL
                     QARIDLLRKRVRT"
     gene            7955..9043
                     /locus_tag="VN041_11945"
     CDS             7955..9043
                     /locus_tag="VN041_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HWU29784.1"
                     /translation="MSAPAHATAADRKQWARYLVEERAEGAVYSRLAARREGEEKEIL
                     LSLAEAERRHEQHWLDLLGGEEPSRLPRAGLRSRMLGWMAGRFGSIFVLALAQSAEAR
                     SPYDSEKYATPAMRADEKVHHEVVRGLAARGRRRLSGSFRAAVFGANDGLVSNLALVL
                     GIGATGVGSGLVLFSGIAGLLAGALSMGAGEFVSVRSQRELLAATEESNDADATAGDL
                     DIDANELALVYRARGMDEDESLARAQRVMSAAQAGIRRDATGPIGVPNDHEIVGSDWT
                     AAISSFLLFASGAIVPVLPWLFGLTGFTAILVALVLVGVALLSTGAMVGILSGGPPLK
                     RALRQLAIGFGAAAITYVLGLVFGVGAV"
     gene            9147..9602
                     /locus_tag="VN041_11950"
     CDS             9147..9602
                     /locus_tag="VN041_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU29785.1"
                     /translation="MAQIIETVDVDVPVRVAYDQWTQFESFPDFLEEVVSITQVDDTR
                     NHWVVEVGGVRREFDTEITEQHPDDRVAWRSVGGETAHAGVVTFHRLTDDSSRVTVQI
                     DWKPEGLAEMVGSMAGLASHAVRKDLGNFKDFIENRSRATGAWRGDVDA"
     gene            complement(9649..9733)
                     /locus_tag="VN041_11955"
     tRNA            complement(9649..9733)
                     /locus_tag="VN041_11955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9697..9699),aa:Leu,seq:gag)
     gene            9844..11139
                     /locus_tag="VN041_11960"
     CDS             9844..11139
                     /locus_tag="VN041_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU29786.1"
                     /translation="MSDSSLPEVARIARDLIRIDTSNFGGGNSKGEREAAEYVGAYLA
                     SLGLEPEYYEPIPNRTNVMARVRGRNPERPALVVHGHLDVVPAMAEDWSVDPFEGVIR
                     DGMLWGRGAVDMKNMDAMILTAVAELLRAGEQPDRDLILVFFADEENGGVEGSALVVR
                     DRPEWFSGATEAISEVGGYSITVDDHRAYLLQVGEKALMWLRLVAKGRAGHGSRYHEQ
                     NAVTRLAEAVAAVGRTRWPIRLTPTTQALLDGLSELTGRPIDDPDALAAAAGPAEAFL
                     RSTFRTTANPTALTAGYKHNVIPETAEALIDVRVIPGTEDDVLAELQRIVGDDIRIET
                     VVRDIGMETPFTGELVDAMVAALGKHDPGVPVIPYLLGAGTDNKALASIGITGYGFAP
                     LQLPADLDFTGMFHGVDERVPVESLVFGQRVLTDLLRSC"
     gene            11188..12018
                     /locus_tag="VN041_11965"
     CDS             11188..12018
                     /locus_tag="VN041_11965"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWU29787.1"
                     /translation="MNLLEALLLGVVQGLTEFLPISSSAHLRIVGALLPSGEDPGAAF
                     TAITQIGTEAAVVVFFWRDIVRIIGTWFRSLTGKAPRNDPDAKMGWLIILGSIPIVLL
                     GLLFQDKIETTLRSLWIVAIMLIGFGLLLGIADYIGAKKRKLDELTYPHGIAYGVAQA
                     FALVPGVSRSGGTITMGLFLGYERAAAARYAFLLAIPAVFGSGFYQLFKHGGEAGQYF
                     SLGETFAATGIAFVVALGVIAFFMNWISKRSFLPFVIYRVLLGGTLIVLLSTGVLSPR
                     "
     gene            complement(12023..>12238)
                     /locus_tag="VN041_11970"
     CDS             complement(12023..>12238)
                     /locus_tag="VN041_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWU29788.1"
                     /translation="VGPAREHLRTAAAAWEASIDAAAAEDEDMTEYIHQLERTRDTWD
                     SPDASGDAIAQAFERYLRRRGDGPDKK"
BASE COUNT         1835 a         4237 c         4311 g         1855 t
ORIGIN      
        1 atgcagcgcc ggctcgcagc gctggagcgc gcgtcgggcc cgcgtgagcc cgaagggcgc
       61 gcccccatgc ccggttcagt cgtggcgctg cacgtcgcag acggtgacac cgtccgcgaa
      121 ggagaccccc tcgtctcgat cgaggcgatg aagatggagc atccggtgct cgcgccgcac
      181 gacggcacgg tgcacctgct ggtcgccgtc ggcgatcagg tacggcgcga ccagccggtc
      241 gcccgcgtca gcgaagagga gacatcatga ccgacgacct cagcagcatc ggcctgaacg
      301 aggagcagcg cgaactcgcc gccatggtgc gcgacttcgc cgagcaggtg gtggcaccgc
      361 aggcctacga ggccgatcgc tcccacaccc tcaacctcga cgtggtgcgg cagatgggcg
      421 aactgggcct gttcggcctg ccgttctcgg aggagtacgg cgggcagggc ggcgactacc
      481 tggccctggg actggccatc gaaggtctcg cccgcgtgga ccagtccatc gccatcacgc
      541 tcgaggccgg tgtgagcctg ggcgccatgc cgatctaccg gttcggcacc gaggagcaga
      601 agcaggaata cctcccctcg ctgctggctg cggagaacct cgccggattc ggcctgaccg
      661 agccggaggc cggcagcgac gccggcgcca cccgcaccac cgcccgactc gagggcgggg
      721 agtggatcat cgacgggtcc aagcagttca tcaccaactc cggcacggcg atcacgcgct
      781 tcgtgacggt cacggccgtg acgggggtga ccgacgggcg caaggagatc tccacgatca
      841 tcgtgcccaa cggcaccccg ggcttcacgg tggagcccgc ctacgacaag gtcggctgga
      901 acgcgtccga cacccacccg ctcaccttcc agggcgtgcg cgtcccggag ggcaacctgc
      961 tgggcgagcg cggtcgcggg ttcaagaact tcctcagcat cctcgacgag ggacgcatcg
     1021 ccatcgccgc tctcgccacg ggcgccgcgg agggctgcct ggaggcggcc gtcgactacg
     1081 cccagaagcg caccatcttc ggatccgccc tgagcacccg ccagaacgcg cagttcacgc
     1141 tggcgcgcat gcgcgcccgc gtgcacacgg ctcggctggc ctggcaccac gccgcgcgtc
     1201 tgcgcgacgc cggactgccg ttcgccgagg aggcggccat cgccaagctc gtctccggtg
     1261 acgccgccat ggacaacgcc cgggatgcga cccagatctt cggcggcaac ggtttcatga
     1321 acgagtaccc ggtggggcgc cactaccgcg actcgaagat cctcgagatc ggtgagggca
     1381 ccaccgaggt gcagctcctg gtcatcgccc gcaagctcgg cctggccggc tgaatccgca
     1441 gaggagctag ggtgcgatca tgaccgacat cgtccagcgc ggcctgtact tcgaggagtt
     1501 cgaactcggc gcccggtacc tgcatcgtcc gggccgcacc gcgaccgagg ccgacaacgt
     1561 gctgttcacg accctcacca tgaacacgca ggccctgcac ctggatgcag cattcgccgg
     1621 tgccgagagg ccgttcgggc agcgcctgat gaactcgatg tggacgctgt cgaccatggt
     1681 cggctcctcg gtcgcgcagc tcacccaggg gacgctggtg gcgcagctgg gactcagcga
     1741 catcaccttt ccgcatccgg tcttccatgg cgacacgctg tacacggaga gcgtgatcac
     1801 cgacaagcgc ctgtcgtcgt cccggcccgg gcaggggatc gtcacgatcg ctcatacggg
     1861 tcgcaaccag gacggcgtga tcgtcgcgac cgccacgcgc gtggcgctga tgcactgcct
     1921 gccggaaggg gaatgatgac cttcgagctc ggacccgccc tgctgttctg cccggccgac
     1981 cgtccggaac gctacgccgg agggcttgcg aaagcggatg ctgtgatcct cgacctcgag
     2041 gacgcggtgc ttccggatgc caaggcgcag gcgcgcgaac acctcgtggc ggcggatctc
     2101 gaccccgcgc gggtgatcgt gcgggtcaac gatccggcat ccgctcattt caccgccgac
     2161 ctcgaggtcg tggcgcgcac tccgttccgc accgtgatgg tcgccaagac cgaggacccg
     2221 tccgcgctgg acgccttcgg cgaggagtac gccctgctgg cgctctgcga gaccgcggcg
     2281 ggcgtgctga acgctggggc catcgccgcg catccgcggg tcgcggctct gatgtggggc
     2341 gcggaggatc ttgtggcgtc gatcggcggc accgcgagcc ggttcccgga cggacgctac
     2401 cgagaggtgg cgcgccacgc gcgggcatct gtgctggtcg ccgccgcagc gcacgggaag
     2461 gccgcgatcg acgcggtgca cctggacatc gacgacgagg cggggctggc ggccgaggcg
     2521 gcggatgccg tggcgtcggg attccgtgcc accgcctgca tccacccgaa gcaggtcgcg
     2581 atcatccgcg acgcctaccg tcccgatgcg gcaaccctgg actgggcacg tgcggtgctc
     2641 gccgctgccg atggggagcg gggcgtgttc cgcttccagg ggaggatggt cgacgagccg
     2701 gtgctgcgtc acgcccgggc ggtactgagc agggcctgac ccgaaccgag gcccgtcaga
     2761 ggacgtggac gtcctggagc agccgcaggg agccgggcga cacctcgaag cggtgcagtg
     2821 agccgttccc gagcacactg ccgatcttgg gcagcgtccc gccggaggcg tggtcgatga
     2881 gggagcggat gacgccgccg tgcgtcacga cgatgatcga ctcggccctc ggggcggaac
     2941 gacgccgggc gtcacgggcg atccggtgca gcgcactcat cgcacggatg cacacctcgt
     3001 cgagggtctc cgctccgggg accacggcat gccagtcgcc gtacgcgtcg atgtactcct
     3061 ggaccagcag gccctcgccc tcgccgaact cgcgctcgcg cacatcgtgc acgagcgccg
     3121 gcgctgcgag gccgagctca ccggcgatga tctccgcggt ctcacgtgcg cggagcagcg
     3181 ggctcgcgta gatcgcgtga tgcgtgtccc tggacagccg ccctgcggcc gtgagtgcgt
     3241 ccgcgcgtcc ggtgtcgttc agggggatgt cggtggaccc ctgaattcgc cgagcgaggt
     3301 tccagtcggt ctgcccgtgg cgcacaaggg tgagaagggt cacactccga ccctacgcga
     3361 gagcgcctgc gagggcgctc agcacggggg aggtgccgcc ggcgagcacg gcgtctgccc
     3421 aggcatccgc tctcgtcggc tcgcggttga cgatcaccag ggggatgccg cggcgccgcg
     3481 cgcgctcgat gatgcggatc ccggagttca cggtgagcga ggtgccggcc accagcagcg
     3541 cgtccgctgc ggtcacgacg gactcggccg accggaagcg ggcggcgggg acgaactcgc
     3601 cgaagaagac cacctcgggt ttgagcatgc ccccgcacac cgtgcacggt gggatcacga
     3661 agccctccgt gctctccggg gtcacgtccc cgtcggggtt cagctcgatg ttctcgggct
     3721 tgcggaccca ggggttcagc gcctcgagct ggtcggcgat cgcccggcgg tcgaacacct
     3781 gcccgcagtc caggcacagc gtgcggtgca tggtgccgtg gatctccacc acccgggagc
     3841 ttccggcacg cagatgcagg ccgtcgacgt tctgggtgac gacgccgctc accacgccgg
     3901 cccgctccat ccgcgctagt gccacgtggc cctcgttcgg ttccggtgcc gtgaacgcca
     3961 gccagcccag gtggccgccc agccagtagc ggcggcgagc ggactcgtcg gcgaggtagg
     4021 tctggatggt catcgggttc gcgcgcgtgc gcgccccgac gccgcggtac gcgggaatcc
     4081 ccgaatcggt ggagatgccg gcgccggtga gcacagcgac ccgccgacct ctcagcagct
     4141 ccgcgacatg atcgatgcgc gttcgggtgt cgtcgtcgat cgtgtccggg aactgcacgc
     4201 tcggcagtct acgtctccgg tgcggggccg ggctccgctg gcggggaccc gcacctgaca
     4261 gagtgggagc atgcacgtga ttccggtctc cgatcccgac gacgcgcggt tggatgacta
     4321 ccgctcgctg accgacaccg cgctgcgccg caggaacgag cccgaaggcg ggctctacat
     4381 ggcggagtcc gccaaggtga tcgcgcgggc cgtgcaggcc ggccaccggc cgcgatccgt
     4441 gctcacgcag gagaagtggg tggatgccgt ggccgacgcc ctcgacggcg tcgggagcgg
     4501 ggtcgagacg cccgtgtaca cggcatccgc cgaggtctcc gagacggtca ccggcttcgc
     4561 catccaccgc ggcccgctcg ccgcgatgca ccgcccggcg ctgctttcgg tggcggaggt
     4621 gctgcagcgg gcacgaccgg atcgtgccag gatcgccgtg ctcgaagggc tgaccgacca
     4681 caccaacgtc ggcgccgtgt tccgctccgc cgcggcgctc ggcgtggacg cagtgctcgt
     4741 gacgcccgac tgcgccgacc cgctgtaccg ccgctctgtg cgggtgtcca tgggcaccgt
     4801 gttccaggtg ccgtggacgc gcgttccagc atggccggag ggcatgcgcg agctgcagga
     4861 cgccgggttc gtcgtagcgg gcatgacact gggggagggc gcgatcgagg tggacgatct
     4921 cgtcgccgag gatcaccccc ggctcgcgat ggtcctcggc accgagggcg acgggatccg
     4981 tcctgcgacg gatgccctgc tggatcgccg tgtcacgatt ccgatgatgg gcggcgtgga
     5041 ttcgctgaac gtcgcggcgg catcggccgt cgccttctac gccaccagat gagctcgccg
     5101 cgagagcgga cggtcagggg gccgggcgcc gcccgcgcga tcacctctcg gcgccgggca
     5161 tccgcaccgg agacggctca ggccgcttgg cctcgacgtg gtcgcccgag gactgatgac
     5221 gcagcctgcg cagcacccag gggaccaggt gctcgcgtgc ccagccgatg tcctcggctc
     5281 gcgcttcgcg ccaggtgcgc accgggaagg gatcgggctg catggcctgc aggtcgttcg
     5341 gcacgttgag cgcccgcagc accatccgag cgatctcgtg gtggccgagc gcgttgtagt
     5401 gcagccggtc gtcgtcgaag aagcgcgggt cctgcacgac cttcagcgac cactggtcgg
     5461 cgatgatgca gtcgtaccgg tcgccgatcg cgcgcacgtt ctcgttgtag atggccacct
     5521 tgccgcggaa cgggcggaac accggggtga agccggtgtc gattccggtg aagagcacca
     5581 ccgcggcgcc ggtggaggag agccgagcga cggcgtcctc gagttgcacg gcgatctcgt
     5641 cgggatcggt gcccggacgg atcacgtcat taccgccggc acagatcgag acgagatcgg
     5701 gcttgagcgc gaccgccggc tcgatctggt ccgcgacgat ctgggcgatc agcttgccgc
     5761 gcacggcgag attggcgtag gcgaagtcgt ccacatcctg gccgagcacc tcggcgacgc
     5821 gatcggccca gccgcgcagc ccgccgggca ggtcgggcgc ggggtcgccg acaccctcgg
     5881 tgaacgagtc gccgacggcg acgaaccgtc gccagggatg cggtccctcg ttctcgacga
     5941 acggggtgcg agacgtctcc tggtccacca tcgggttctc cttcggcatc acgcgcccag
     6001 ggtgcgggcg cctgacagag cctacccgcg ctgatcggcg ggcgcgcaga gcgttgtcgg
     6061 cgggctggat tatcgttgag cagtgctctc tccgtccttc cctcagcgtg ccccgtgggg
     6121 taccgccgac aagctgaggg catggcagcg ggaggcgctg gacgcgtatt tccaggccga
     6181 tcagcgggat ttcctcgtcg ccgccacccc gggggcaggc aagaccacct tcgcgctcac
     6241 gctcgcggtc gagctgctgc gcatgggcga ggtcaaccgg gtcatcgtgg tcgcgccgac
     6301 cgagcatctg aagacgcagt gggcggatgc ggccgcgcgc gtccacatcc ggctggaccc
     6361 ccggttccgc aacagtcact gggcgccctc ccggcagtac cacggcgtgg tcgtcaccta
     6421 tgcgcaggtg gcggcgaagt ccagcgtgca ccggcacctg accgaggatg cgaggacgct
     6481 cgtcatcctg gacgaggtgc accacggcgg cgacgctctc agctggggtg acgccatccg
     6541 cgacgcgtac gggccggccc gccgccgact gctgctgtcg ggcacgccgt tccgcagtga
     6601 caccgcgccc atcccgttcg tggagtacat ccccgacgaa tccggcgcac gggtctccag
     6661 caccgactac cgctacggat acgggcgggc tctggccgac ggcgtcgtgc ggccggtgct
     6721 gttccacatg tactccggca agatgcgctg gcgcaccagc gcgggcgacg agctcgaggc
     6781 acatctcgga caggacaaca ccaaggacgt cacctcgcag gcctggcgca ccgccctgga
     6841 cccggagggg gagtggatgc cggcggtgct gtccgccgcc gatcgccgcc tcaccgagat
     6901 ccgccatcac gtcccggatg ccggcgggct cgtgctcgcg acggatcaga cggtcgcgcg
     6961 cgcctacgcc aagatcctgc acagcatcac gggggagcag ccgaccgtgg tgctctccga
     7021 cgatgcgtcg gcgtccgagc gcatcgagaa gttcagcgag acggaccggc gctggatggt
     7081 cgccgtgcgc atggtgtcgg agggcgtcga tgtcccgcgc ctggcggtgg gcgtgtacgc
     7141 gacgtcgtcg tcgacgccgc tgttcttcgc acaggccatc ggccgcttcg tgcgtgcgag
     7201 gcgccgcggt gaggccgcga gcgtgttcct gccgaacgtc cccgtgctga tgaccctcgc
     7261 gaacgagatg gagcgtcagc gcgaccacgc gctcgatcgg cgggacaagg acgacgacgg
     7321 cctcgacgat tcgctgctgg agagcgcgaa ccgcgaggag gacgcgtccg acgccctcac
     7381 ccaggagttc agctatcagg cgatctcgtc gctggcgcac ttcgatcggg tcgtgttcga
     7441 gggcaaggag ttcggtcagc tcgccgaacc cggcaccccc gaggaggagg agttcatcgg
     7501 gttccccggg cttctcgagc ccgagcacgt gcacgagctg ctcatgcagc gccaggccag
     7561 gcagggcaag ctgcgccagg cgcgggaggc ggccaccccg gtcgacacga cgaccctccc
     7621 ggcgcccctg catcgcactc ttcgcgagca gcggcagctg ctgaacagcc tggtcggcct
     7681 gtacgcgcgg cagaccggcg agccgcatgg atccgtgcac gccgagctgc gccggatgtg
     7741 cgggggaccg gctgtggccc aggcgaccgt gtcgcagttg caggcgcgga tcgacctgct
     7801 gcgcaagcgg gtgcggacct gacagacccg cccccggtat cagccgggtg cgggtctggt
     7861 tcgggcgtcc gaaatcctgt caatccgcgt cctgatgcgg ttccgaggtg cccacgtcga
     7921 taccgtggaa tgttgatcta cccacggagg acgcatgtct gcccccgccc acgccactgc
     7981 tgcggaccgc aagcagtggg cccgctatct ggtcgaagaa cgcgccgagg gagccgtgta
     8041 ctcgcggctc gccgctcgga gagaaggcga ggagaaggag atcctgctct ccctcgcgga
     8101 agccgagcgt cgccacgagc agcactggct cgatctgctc ggaggggagg agccctcccg
     8161 gcttccccgg gccggtctcc gctcccggat gctgggctgg atggccggcc ggttcggatc
     8221 catcttcgtg ctcgccctcg cccagagcgc cgaggcgcgt tccccgtacg acagcgagaa
     8281 gtacgccacc ccggcgatgc gggccgatga gaaggtgcac cacgaagtgg tccgcggcct
     8341 cgccgcgcgc ggacgccgcc gactgtccgg ctcgttccgc gcggcggtct tcggcgcgaa
     8401 cgacggactc gtctcgaacc tcgcactggt gctcggcatc ggggcgacag gggtcggctc
     8461 cggactggtg ctcttcagcg gcatcgccgg ccttctcgcc ggtgcgctgt cgatgggtgc
     8521 gggagagttc gtctccgtgc gctcccagcg cgagctgctg gccgcgacag aggagagcaa
     8581 cgacgcggat gccaccgcgg gcgacctcga catcgatgcc aacgagctcg ccctcgtgta
     8641 ccgggcgcgc ggcatggatg aggacgagtc gctggcacgc gcccagcggg tcatgagcgc
     8701 cgcgcaggcg ggcatccgtc gtgacgcgac cggaccgatc ggcgtgccga acgatcatga
     8761 gatcgtgggc agcgactgga cggccgccat ctccagcttc ctgctgttcg cgtccggtgc
     8821 gatcgtgccc gtgctgccgt ggctcttcgg gctcacgggc ttcacggcga tcctcgtcgc
     8881 gctggtgctc gtcggagtcg cgctgctgag cacgggtgcg atggtcggca tcctctccgg
     8941 cggcccgccg ctcaagcgcg cgctgcggca gctggcgatc ggcttcggag cggcggcgat
     9001 cacctacgtg ctcggtctcg tgttcggcgt cggcgcggtg tgagccgggc catgcgctga
     9061 gtcatccgcg tgccgtcaac cccctttccg ggctcggcga catgcggatg actggatgct
     9121 ccacaccgaa gagcaaggag cagagcatgg cccagatcat cgagaccgtg gatgtcgacg
     9181 tccccgtgcg cgtcgcgtac gaccagtgga cccagttcga gagcttcccc gacttcctcg
     9241 aggaggtggt ctcgatcacc caggtggatg acacgcgcaa tcactgggtc gtcgaggtcg
     9301 gcggcgtgcg ccgcgagttc gacaccgaga tcaccgagca gcacccggac gaccgcgtgg
     9361 cctggcgcag cgtcggtggc gagacggccc atgcgggcgt ggtgaccttc caccgcctga
     9421 ccgacgattc cagtcgcgtg accgtgcaga tcgactggaa gccggagggc ctggccgaga
     9481 tggtcggctc gatggcgggc ctcgcatccc acgcggtcag gaaggacctc ggcaacttca
     9541 aggacttcat cgagaaccgc agcagggcga ccggcgcgtg gcgcggcgac gtcgacgcct
     9601 gaacggagaa ggccccggat ctctcgatcc ggggccttct ccgttctgtg cgcgaggggg
     9661 gacttgaacc cccacgccct atgcgggcac tagcacctca agctagcgcg tctacctatt
     9721 ccgccacccg cgcaggtgat gaaaagtcgt tgccgaccga agaatgactc tagcacggtc
     9781 cgcggacagc cacgaaacac gccgacgccc gggcgtggca cgccgtttcg atagcctgga
     9841 gccatgtcgg attcctccct gcccgaagtc gcccggatcg cccgtgatct gatccgcatc
     9901 gacacctcca acttcggcgg agggaactcg aagggcgagc gcgaagcggc ggagtacgtc
     9961 ggtgcctacc tcgcctcgct cgggctcgag ccggagtact acgagccgat cccgaaccgg
    10021 accaacgtga tggcacgcgt caggggccgc aaccccgagc gtcccgcgct ggtcgtgcac
    10081 ggacacctgg acgtggtccc cgccatggcc gaggactgga gcgtcgaccc gttcgagggg
    10141 gtcatccgcg acgggatgct gtgggggcgc ggcgccgtgg acatgaagaa catggacgcc
    10201 atgatcctca ccgcggtcgc cgagctgctg cgggcgggcg agcagcccga ccgcgacctg
    10261 atcctggtgt tcttcgcgga tgaggagaac ggcggcgtcg agggctccgc gctcgtcgtg
    10321 cgcgacaggc cggaatggtt ctccggcgcg accgaggcga tcagcgaggt gggcgggtac
    10381 tcgatcacgg tcgacgatca ccgcgcctat ctgctgcagg tgggggagaa ggccctgatg
    10441 tggcttcggc tcgtcgccaa ggggcgggca gggcacggca gtcgctacca cgagcagaac
    10501 gccgtcacgc gtctcgcgga ggccgtcgcc gccgtcggcc gcacccgctg gcccatccgg
    10561 ctcacaccga ccacccaggc gcttctggac ggcctgagcg agctgaccgg acggccgatc
    10621 gacgacccgg acgcgctggc cgcggccgca ggtcctgccg aggccttcct gcgctcgacg
    10681 ttccgcacca ccgcgaaccc caccgcgctc accgccggct acaagcacaa cgtgatcccc
    10741 gagaccgcag aggccctgat cgacgtccgt gtgatccccg ggaccgagga cgacgtgctc
    10801 gccgagctgc agcggatcgt cggcgacgac atccgcatcg agaccgtcgt gcgcgacatc
    10861 ggcatggaga cgccgttcac cggcgagctg gtggatgcga tggtcgcggc cctggggaag
    10921 cacgaccccg gcgtgccggt gatcccgtac ctgctcggcg ccggcaccga caacaaggcg
    10981 ctggcctcga tcggcatcac cgggtacgga ttcgcgccgc tgcagctgcc cgccgatctc
    11041 gacttcacag ggatgttcca cggagtggac gagcgcgtcc ccgtagaatc tcttgtcttc
    11101 ggtcagcgcg tgctgaccga tctgctgcgc tcctgctgac cggcatccgt ccggggacac
    11161 cgacgcctca cccttggaaa gcaccgcatg aacctgctcg aagcccttct gctcggcgtc
    11221 gtccagggac tcaccgagtt cctgccgatc tcctccagcg cgcatctgcg catcgtcggg
    11281 gcgctgcttc cctccggtga ggacccgggt gccgcgttca ccgcgatcac ccagatcggc
    11341 accgaggcgg ccgtcgtggt gttcttctgg cgcgacatcg ttcgcatcat cggcacgtgg
    11401 ttccggtccc tgaccggcaa ggcgccgcgc aacgaccccg atgcgaagat ggggtggctg
    11461 atcatcctcg gcagcatccc gatcgtcctg ctggggctgc tgttccagga caagatcgag
    11521 acgacgctgc gatcgctctg gatcgtcgcg atcatgctca tcggcttcgg tctgctgctc
    11581 ggcatcgcgg actacatcgg cgccaagaag cgcaagctcg acgagctgac ctacccgcac
    11641 ggcatcgcct acggtgtggc gcaggcgttc gctctggtac ccggcgtgtc gcgatccggc
    11701 ggcaccatca cgatgggcct cttcctcggc tatgagcgcg ctgcggccgc ccgctacgcg
    11761 ttcctgctgg cgattcctgc ggtgttcggc agcgggttct accagttgtt caagcacggc
    11821 ggcgaggccg gccagtactt cagcctgggg gagaccttcg cggcgacggg catcgccttc
    11881 gtcgtggcgc tcggcgtgat cgcgttcttc atgaactgga tctccaagcg cagcttcctg
    11941 ccgttcgtga tctaccgggt gctgctgggc ggcacgctca tcgtgctgct gtccacgggc
    12001 gtgctctcgc ctcgctgatc ggtcacttct tgtcggggcc gtcgccccgg cggcgcaggt
    12061 agcgctcgaa ggcctgggcg atggcgtcgc ccgaggcatc gggagagtcc caggtgtcgc
    12121 gtgtccgctc cagctggtgg atgtactcgg tcatgtcctc gtcctcggcc gcagcggcgt
    12181 cgatcgacgc ctcccaggcc gcggccgcgg tgcgcaggtg ctcgcgggcg gggccgac
//
LOCUS       DATMSX010000390         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_119968,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000390 DATMSX010000000
VERSION     DATMSX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119968"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VN041_11975"
     CDS             <1..419
                     /locus_tag="VN041_11975"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWU29789.1"
                     /translation="AAAWAILVQVEHGGSPLSWTFLALAVVAAVAGWIAARARREGFA
                     FLGFAGFGLFGVGAIFAGMFPLVLPSTVDAAFDLTVWNAANGPYTLGVMTVVAAVSLP
                     VILLYQAWSYWIFRKRVTPGMIPEVHIVLPAVLRAR"
     gene            complement(519..992)
                     /locus_tag="VN041_11980"
     CDS             complement(519..992)
                     /locus_tag="VN041_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWU29790.1"
                     /translation="MTVIIAITAAFAVLGLAVIFGADVVGLLVQRSVYADLDDRALVQ
                     VVGRGHYYADRRMPGVGIPATILTAVTAAAAFIWGTTLLGILSAAGLVLLVTWLLVYA
                     RIAKPINTTLTAAALADEVPADVRSLQARWDSLLPLRVSLIAVALLLLLTTLALL"
     gene            1053..1619
                     /locus_tag="VN041_11985"
     CDS             1053..1619
                     /locus_tag="VN041_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29791.1"
                     /translation="MPRAAQDIERIRERERRIVRAAREIAEEEGWTAVTVRRLADAIG
                     FSQPILYRHFPRGREEIIDQVVIDGYTELLAAIGAGGEGLAPLVTAYLAFARENPAVY
                     EAMTSARTGVVFASAETPEILREGFAMFERAVGGGTPRHRAVRAELLWSTLHGASRLD
                     ADGRFDAALDDIRADEIIALFSAPRTES"
     gene            1804..1962
                     /locus_tag="VN041_11990"
     CDS             1804..1962
                     /locus_tag="VN041_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29792.1"
                     /translation="MWTLLIVLLVLWLGLSVLGFVVKGLLWLAVLGIVLFLVTLVFGL
                     FRRGRSEG"
     gene            1983..2342
                     /locus_tag="VN041_11995"
     CDS             1983..2342
                     /locus_tag="VN041_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU29793.1"
                     /translation="MLSLDPLLSLERAGWDALCDSRGGAFYGELMTEDAVMVLVHGMV
                     LDRAAVVAAMDDAPPWAGYALSDARVVPIGADSAALVYRARAEREGQEPFVALMTSVY
                     TAVDGRTRLALYQQTAV"
     gene            2427..3023
                     /locus_tag="VN041_12000"
     CDS             2427..3023
                     /locus_tag="VN041_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWU29794.1"
                     /translation="MMYEHLGARPRIHDTAVVAPTAVISGDVEIGPNCQVLHGAVITA
                     EGGPITLGEHVIVMENALIRATAANAVHIGDHTLVGTLASIAGATVGEEVFFASGARV
                     FNGALVGSHCEIRVNAIVQRRAVLPEGTVVPIGWIAAGHPVRMFSPDQADEIAEAMPD
                     LDFPGHVFGVDRDTPDLMVQLTERYASSLARHADDRQV"
     gene            complement(3146..4462)
                     /gene="gdhA"
                     /locus_tag="VN041_12005"
     CDS             complement(3146..4462)
                     /gene="gdhA"
                     /locus_tag="VN041_12005"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HWU29795.1"
                     /translation="MFGTALARSPHEPEFHQAVHEVLHSIGPVLERHPKYIENGILER
                     LVEPERQILFRVPWVDDVGKLQVNRGYRIQFSSVLGPYKGGLRFHSSVNLSIIKFLGF
                     EQIFKNALTGQGIGGGKGGSDFDPHGRSDAEVMRFCQSFMSELYRHLGEHTDVPAGDI
                     GVGGREIGYLFGQYRKITNRHESGMFTGKGIGWGGAQVRTEATGYGAVFFVQSMLAVH
                     GQTLEDKRVAVSGSGNVAIYAIEKAQQLGARAITASDSSGYVVDDAGIDLGLLRQIKE
                     VERGRIVEYANRRPGARFVEGGNVWEVPVDIAVPSATQNELNLDSARMLIANGVRAVA
                     EGANMPSTPEAVGAFQDSDVLFAPGKAANAGGVATSALEMSQNASRQRWNFDHSEQKL
                     RDIMTDIHDASFRAAERYGTPGDYVAGANIAGFERVADAMLAQGVI"
     gene            complement(4653..>6282)
                     /locus_tag="VN041_12010"
     CDS             complement(4653..>6282)
                     /locus_tag="VN041_12010"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWU29796.1"
                     /translation="KRSPDEPHRRVVLSLAHRVHATRVAGDDAYSAPEELLADLRTVQ
                     SSLATAGAARHAFGGVQHLIWQVETYGFHLTELEVRQHSQVHAEALAELDAGGELSER
                     TQEVLEVFRAVADIQKRYGLRAAGRYVVSFTRSAADLAAVHRLAAYALGEDAPVLDVV
                     PLFETFADLQAAPGILAEVVEFPEFRARLDATGNRLEVMLGYSDSSKDVGPVAANLAL
                     YEAQRDIAQWAVDAGIRLTLFHGRGGALGRGGGPANSAILAQPPHSVDGRFKLTEQGE
                     VIFARYGEPAIAMRHIDQVAAATLLASSPTVEQRTSGAAERFAPIAATMDTASRERFF
                     SLVAAEGFAPWFATVTPMEEIGLLALGSRPARRGLSVESLEDLRAIPWVFAWTQARIN
                     LAGWFGLGTALEAVGDEDALREAYREWPLLHTMVDNVAMSLAKTDERIARQYLALGDR
                     DDLAAIVLDELRLTREWVIRLTGGDALLQNKPILQRAVQLRTPYVDALSLLQLRALRA
                     LRTPSTVEPVPDADLQRLLLLSVSGVAAGLQNTG"
BASE COUNT          913 a         2104 c         2301 g          964 t
ORIGIN      
        1 ctgcggcggc gtgggcgatc ctcgtgcagg tcgagcacgg cgggagcccg ctgtcgtgga
       61 cgttcctcgc cctggccgtc gtcgccgcgg tggccggctg gatcgccgca cgggcccgcc
      121 gtgaggggtt cgcattcctg ggattcgccg gtttcgggct cttcggcgtc ggcgcgatct
      181 tcgccgggat gttcccgctc gtgctgccct cgaccgtgga cgccgccttc gacctcaccg
      241 tctggaacgc ggccaacggc ccgtacacgc tgggcgtcat gaccgtggtt gccgccgtgt
      301 cgctgcccgt gatcctgctg taccaggcct ggagttactg gatcttccgc aagcgggtga
      361 ccccgggcat gatccccgag gtgcacatag tgctcccggc cgtcctccgc gcgcgctgac
      421 gcgatcgacg ccccgccggc cgggcctggt ggcaggaccg gccggcggag tgctcgtggg
      481 cgtcatcatc gactggcgga gtccgcgagc gggggatctc acagcagggc gagcgtggtc
      541 agcagcagga gcagggccac cgcgatgagc gacacccgca gcgggaggag cgaatcccag
      601 cgggcctgca gggatcggac gtcggcgggc acctcgtcgg cgagagccgc cgcggtcagc
      661 gtggtgttga tcggcttcgc gatgcgggcg tagacgagca gccaggtgac gagcagcacc
      721 agcccggctg cggagaggat gcccaggagc gtcgtacccc agatgaacgc ggcggcggcc
      781 gtgaccgcgg tgaggatcgt cgcggggatg ccgacacccg gcatcctgcg gtctgcgtag
      841 taatggccgc gaccgaccac ctggaccagg gcgcggtcgt cgaggtcggc atagacggag
      901 cgctggacga gcaggccgac cacgtccgcg ccgaagatga cggcgaggcc gaggacggcg
      961 aacgccgcgg tgatggcgat gatgacggtc atggttcctc cggttcgatt ggtgttagcg
     1021 atgctaactg ttagcggtgc taatctaacc tcatgccccg agcagcgcaa gacatcgagc
     1081 ggatccgcga gcgggaacgc cggatcgtcc gtgctgcccg cgagatcgcg gaggaggagg
     1141 gctggaccgc cgtcaccgtg cggcgcctcg ccgacgccat cggcttcagc cagccgatcc
     1201 tgtaccggca cttcccgcgc gggcgggagg agatcatcga ccaggtcgtc atcgacggct
     1261 acaccgagct cctcgcggcg atcggcgccg ggggtgaggg cctggcgccc ctcgtcacgg
     1321 cctacctcgc cttcgcacgc gagaatcccg cggtctacga ggcgatgacc tcggcgcgca
     1381 caggcgtcgt gttcgcctcc gccgagacac ccgagatcct gcgggagggg ttcgcgatgt
     1441 tcgagcgggc cgtcggcggg ggcacaccgc ggcaccgcgc ggtgcgcgcc gagctgctgt
     1501 ggagcacgct gcacggcgcc agccggctcg acgcggatgg gcgcttcgat gccgcgctcg
     1561 acgacatccg cgccgacgag atcatcgcgc tcttcagtgc cccgcggacg gagtcctgag
     1621 caccggagcc tcagcagcct gcaccgccga cctctggatc tgcggccgcc cggagccgac
     1681 cagcacgacc gtcgctgaag gcatccggaa tgcatatcgc gcgtcgtgag cccgccgtag
     1741 cggacgcgga tgccgcggtc aacgccctgt gcttcgtgcc ccgcccgacg catgctggcg
     1801 gacatgtgga ctctgctgat cgtgctgctg gtgctgtggc tgggactgtc ggtcctggga
     1861 ttcgtggtga aggggctgct ctggctcgcg gtgctgggca tcgtgctgtt cctggtcacc
     1921 ctggtgttcg gcctcttccg gagaggacga tccgaggggt gatgcgggac gcagaggatc
     1981 ggatgctctc actcgacccc cttctgtcgt tggagagggc gggatgggac gcgctgtgcg
     2041 actcgcgtgg cggcgccttc tacggagagc tgatgacgga ggatgcggtg atggtcctgg
     2101 tccacggcat ggtgctggat cgtgccgccg tggtcgccgc gatggatgac gcgccgccgt
     2161 gggccggtta tgcgctttcg gatgccaggg tggtgccgat cggcgcggac tccgccgcac
     2221 tcgtgtaccg cgccagggcg gagcgcgagg gccaggagcc gttcgtggcg ctgatgacca
     2281 gcgtgtacac ggccgtcgac gggaggacgc ggctcgcgct ctatcagcag accgcggtct
     2341 gatccgccag accagttcgc gcgatcgcga gggacccgcc gccattccgt actgaccgtg
     2401 tgcgcgggcc ggactagtct ggccgcatga tgtacgagca cctcggggct cggccccgca
     2461 tccacgacac cgccgtcgtg gcgccgaccg ctgtgatctc cggcgacgtc gagatcggac
     2521 cgaactgcca ggtgctgcac ggcgccgtga tcaccgccga gggcgggccg atcacgctgg
     2581 gcgagcacgt catcgtgatg gagaacgcgc tcatccgcgc gaccgccgcc aacgccgtgc
     2641 acatcggcga tcacacgctg gtgggaaccc tcgcgagcat cgccggcgct acggtcggcg
     2701 aggaggtgtt cttcgcctcc ggcgcccgcg tgttcaacgg ggcgctggtc ggctcgcact
     2761 gcgagatccg cgtgaacgcg atcgtgcagc ggcgcgcggt cctgccggag ggcacggtgg
     2821 tgccgatcgg ctggatcgcg gccggccatc cggtgcggat gttctcgccc gaccaggccg
     2881 acgagatcgc ggaggcgatg ccggacctgg acttccccgg tcatgtcttc ggcgtcgacc
     2941 gcgacacccc cgatctgatg gtgcagctca cggagcgcta cgccagctcg ctggcccggc
     3001 acgccgacga ccgccaggtg tgatgctcgc ggagcatctc accgagccac ccgccgaaac
     3061 atcgagccac cacgcaggat cacgtgatct gcgtggtggc tcggtgcttg cgtggtggcc
     3121 tggcggtttc gccggccgag tcgggtcaga tgacgccctg ggccagcatc gcgtcggcga
     3181 cgcgctcgaa accggcgatg ttggcgcccg cgacgtagtc gccgggggtg ccgtagcgct
     3241 cggcggcgcg gaaggacgcg tcgtggatgt ccgtcatgat gtcgcgcagc ttctgctcgc
     3301 tgtgatcgaa gttccagcgc tggcgcgagg cgttctggct catctcgagc gcggaggtgg
     3361 cgacgccgcc ggcgttcgcg gccttgcccg gcgcgaacag cacgtcggag tcctggaagg
     3421 cgcccacggc ctccggagtc gacggcatgt tggcgccttc ggccacggca cgcacgccat
     3481 tggcgatcag catccgggcg gagtcgaggt tcagctcgtt ctgcgtggcg gagggcacgg
     3541 cgatgtcgac cggcacctcc cacacgttcc cgccctcgac gaaccgggcg ccggggcggc
     3601 ggttcgcgta ctcgacgatg cggccgcgct cgacctcctt gatctggcgc agcagcccca
     3661 ggtcgatgcc ggcgtcgtcg acgacgtagc cggaggagtc ggatgccgtg atcgcgcgtg
     3721 cgccgagctg ctgggccttc tcgatcgcgt agatcgcgac gttgcccgag ccggagacgg
     3781 cgacgcgttt gtcctcgagg gtctggccgt ggacggcgag catcgactgc acgaagaaga
     3841 ccgcgccgta accggtggcc tcggtgcgca cctgggcccc accccagccg atgcccttcc
     3901 cggtgaacat gccggactca tgccggttgg tgatcttgcg gtactgaccg aagaggtagc
     3961 cgatctcgcg gccacccacc ccgatgtcgc ctgcggggac gtcggtgtgc tcgccgaggt
     4021 gacggtacag ctcgctcatg aacgactggc agaagcgcat cacctcggca tcgctgcggc
     4081 cgtgcgggtc gaagtcggag ccgcccttgc cgccgccgat gccctggccg gtgagcgcgt
     4141 tcttgaagat ctgctcgaag ccgaggaact tgatgatcga gaggttcacc gacgagtgga
     4201 agcgcaaccc gcccttgtac gggccgagca cggacgagaa ctggatacgg tagccgcggt
     4261 tcacctgcag ctttccgacg tcgtcgaccc agggcacgcg gaacaggatc tgacgctccg
     4321 gctccacgag ccgctcaagg atgccgttct cgatgtactt cgggtgcctc tcgagcaccg
     4381 ggccgatcga gtggagcacc tcgtgcaccg cctggtggaa ctccggctcg tgcgggctcc
     4441 gagcgagggc cgtgccgaag atcggctgtg cggcagcgct gagcggatgg aagtcggcag
     4501 tggtttcggt cacgcgggaa gctttctcgg agagggatgg gggatgcgag cgatcgtgtc
     4561 gcatggatgc ttcgccggat caggctgatt ttcaccctac aacgatcacc ggagggccct
     4621 ggcggacgtg tcagccggcc ccgcggcagg tctcacccgg tgttctgcag gcctgccgcg
     4681 accccgctga ccgagagcag cagcaggcgc tgcaggtcgg catccggcac gggttcgacg
     4741 gtcgagggcg tccgcagggc gcgcagcgcc cgcagctgaa gcagggagag cgcgtccacg
     4801 tacggggtgc gcagctgcac ggcgcgctgc aggatcggct tgttctgcag cagtgcgtcg
     4861 ccgcccgtga gccggatgac ccattcgcgg gtcaatcgca gctcgtcgag cacgatggcg
     4921 gcgaggtcgt cgcgatcgcc cagcgccagg tactgccgtg cgatgcgctc atcggtcttc
     4981 gccaggctca tcgccacgtt gtcgaccatg gtgtgcagca gcggccattc gcggtacgcc
     5041 tcgcgcagtg cgtcctcgtc gccgacggcc tccagggccg tgccgagtcc gaaccagccg
     5101 gcgaggttga tgcgcgcctg cgtccatgcg aacacccacg ggatggcgcg cagatcctcc
     5161 agtgactcga cggagagtcc gcgccgcgca ggacgggagc ccagggcgag caggccgatc
     5221 tcctccatgg gagtgacggt cgcgaaccag ggggcgaagc cctcggccgc caccagcgag
     5281 aagaaccgct cacgggacgc cgtgtccatc gtggcggcga tcggtgcgaa gcgctcggcg
     5341 gcaccactgg tgcgctgctc gaccgtcggc gaggacgcca gcagggtcgc ggcggccacc
     5401 tgatcgatgt gccgcatcgc gatggcgggc tcgccgtagc gggcgaagat cacctcgccc
     5461 tgctcggtga gcttgaaccg gccgtcgacg gagtgcggcg gctgggcgag gatcgccgag
     5521 ttcgcagggc cgccgccgcg gccgagcgcg ccgccacgac cgtggaagag ggtgagccgg
     5581 atgccggcat ccaccgccca ctgcgcgatg tcgcgctgcg cctcgtagag cgcgaggttc
     5641 gcggccaccg ggccgacgtc cttcgacgag tccgaatagc cgagcatgac ctcgaggcgg
     5701 ttgccggtgg catccagccg ggcacggaac tccgggaatt ccacgacctc ggcgaggatg
     5761 ccgggggcgg cctgcaggtc ggcgaacgtc tcgaacagcg ggacgacgtc gagcaccggc
     5821 gcatcctcgc ccagtgcgta ggcggcgagg cggtgcacgg cggcgaggtc ggctgctgac
     5881 cgggtgaacg agaccacgta gcgccccgcg gcccgcagcc cgtagcgctt ctggatgtcc
     5941 gccaccgcgc ggaagacctc gagcacctcc tgggtgcgct cgctgagctc gccgcccgca
     6001 tccagctccg cgagcgcctc ggcgtgcacc tgtgagtgct gtcgcacctc gagctcggtc
     6061 aggtggaagc cgtaggtctc cacctgccag atcagatgct gcacgccgcc gaacgcatgc
     6121 cgggcggcgc cggcggtggc cagcgaggac tgcacggtcc tcagatcggc cagcagctcc
     6181 tcgggtgcgg agtaggcatc gtcgccggcg acccgggtgg cgtgcacgcg atgcgcgagc
     6241 gagagcacga cgcgacggtg cggctcgtcg ggggagcgct tc
//
LOCUS       DATMSX010000391        14325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_157904,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000391 DATMSX010000000
VERSION     DATMSX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14325
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157904"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VN041_12015"
     CDS             complement(<1..136)
                     /locus_tag="VN041_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HWU29797.1"
                     /translation="MSTPFGTRLRAALDAHGPLCVGIDPHAALLEQWGLTQDADGLRA
                     F"
     gene            complement(133..3420)
                     /gene="carB"
                     /locus_tag="VN041_12020"
     CDS             complement(133..3420)
                     /gene="carB"
                     /locus_tag="VN041_12020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWU29798.1"
                     /translation="MPKRDDITSVLVIGSGPIVIGQACEFDYSGTQACRVLREEGVRV
                     ILVNSNPATIMTDPDFADATYIEPITPEVIETIIAKEKPDAILPTLGGQTALNAAMAL
                     HERGILEKYDVELIGAKVDAIRKGEDRQIFKELVIEAGADVAKSVICHTMDELLAGAA
                     ELGYPLVVRPSFTMGGLGSGFAFDEADLRRIGGAGLHDSPTTEVLLEESILGWKEYEL
                     ELMRDTADNTVVVCSIENVDPVGVHTGDSITVAPALTLTDREYQKMRDISIDIIRAVG
                     VDTGGCNIQFAVAPTNGRIIVIEMNPRVSRSSALASKATGFPIAKLAAKLALGYRLDE
                     IPNDITGVTPASFEPTLDYVVVKVPRFAFEKFPAADATLTTTMKSVGEAMAIGRNYTT
                     ALQKALRSLEQRGSSFHWGPEERSVEELLEIAKTPTDGRIVVLQQALRKGATIEQAFD
                     ATAMDPWFLDQIVLINEVAQSVAQAAELDAATLRHAKEHGFSDAQIAQLRGISEPEVR
                     GIRHALGLRPVYKTVDTCAGEFPALTPYHYSSYDFETEVAPSERTKVVIIGSGPNRIG
                     QGVEFDYSCVHASFALSDAGYETVMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEVLD
                     AEAQSGTILGVVCQLGGQTPLGLAKGIEAAGYTVLGTSPAAIDLAEERELFSRLLDDA
                     GLVAPRNGTAIDVEGAVRIAEEIGYPVLVRPSFVLGGRGMEIVYDTASLRDYFVRTAG
                     NVVIEEGKPLLVDRFLDDAIELDVDALYDGTDLYIGGIMEHLEEAGIHSGDSSCTLPP
                     ISLGRTQIERVRAATLAIAEGVGVRGLLNVQFAISAGVLYVIEANPRASRTVPFVSKA
                     LGVPMAKAASRVMAGTSIAELIAEGMLPAQDGSSVPLGAPIAVKEAVLPFKRFRTHDG
                     KVVDSVLGPEMRSTGEVMGIAADFPTAFAKSQAAAYGGMPTSGTVFISVADSDKRDVI
                     LPAHRLAQLGFSLVATEGTAEILSRNGIQVALAAKYSETQENGERNIVDLINDGEIDI
                     IVNTPSGGAARADGYEIRAAAVAADKALFTTMAVLGAAVSGMDAANEGFSVRSLQEYE
                     LDRQAAE"
     gene            complement(3420..4592)
                     /gene="carA"
                     /locus_tag="VN041_12025"
     CDS             complement(3420..4592)
                     /gene="carA"
                     /locus_tag="VN041_12025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWU29799.1"
                     /translation="MSNPTTGAPSPLTSAVLVLEDGTRFTGRAYGAVGTTLGEVVFAT
                     GMTGYQETLTDPSYAGQIVLQTAPHIGNTGMNREDDESARIWVAGYIVRDPARRVSNW
                     RAEESLDDVLIRDGIVGISGIDTRALTRRIRDTGSMRGGIFSGPDAELDAEEQLRRVT
                     EAPQMAGQNLSAEVSVSEPTVIPAEGERIGNLAVLDLGVKQSTLDNLARLGFDVHVLP
                     QSASFADIQAVDPVGVFYSNGPGDPSASDNQVAVLRQVLDAKLPYFGICFGNQLFGRA
                     LGLGTYKLTFGHRGINQPVLDRATGRVEITAHNHGFAVEAPLHGTFASPNGYGTVEVS
                     HVGLNDQVVEGLRAHDIPAFSVQYHPEAAAGPHDAHYLFARFRDLVLADIAGKDAE"
     gene            complement(4589..5095)
                     /locus_tag="VN041_12030"
     CDS             complement(4589..5095)
                     /locus_tag="VN041_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29800.1"
                     /translation="MTREAAVLITIAVALLVLAAMFFAWRRRISRDSALTAPLGVPEH
                     ADVIDRHEVLYVATTVHDQPLERIAMKALTYRARGELVLTDRGVALSLDGSPTVFIAS
                     ERLLAADLATVAIDRVVEPNGLIRLVWRVAEGTIVDSFLRPASVGPQQILPQLQRLSA
                     GSENGEQS"
     gene            complement(5092..6390)
                     /locus_tag="VN041_12035"
     CDS             complement(5092..6390)
                     /locus_tag="VN041_12035"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWU29801.1"
                     /translation="MNRNLVITGAQLLGGDSADIAIEDGVIVEIGAGLTRSGARVIDA
                     AGLVALPGLVDLHTHLREPGFEASETVLTGTQAAAAGGFTAVFAMPNTQPVADTAGVV
                     EQELALGEAAGYATVQPIGAVTVGQKGERLAELGAMAGSRARVRVFSDDGFCVWDPLI
                     MRRALEYVKAFDGVIAQHAQDPRLTQGAQMNEGIVSAELGLAGWPAVAEESIIARDVL
                     LAEHVGSRLHVCHLSTAGSVDIIRWAKKRGIRVTAEVTPHHLLLTDELVRDYDARYKV
                     NPPLRTAEDVTAVREGLADGTIDIVATDHAPHPAENKACEWQVAANGMVGLESALRVV
                     HQSMVATGMLDWTDVARVMSSAPAGIGQLQGFGSLEVGAPAHVTLYDPSVPGVFTETD
                     LHGRSHNSPYLGRDLPGRVQWTFHGGVVTVDDGQLVQELA"
     gene            complement(6387..7352)
                     /locus_tag="VN041_12040"
     CDS             complement(6387..7352)
                     /locus_tag="VN041_12040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWU29802.1"
                     /translation="MRHLLDTRSLERDEALRILDVAEDMAETQSRQIKKLPTLLGKTV
                     VNLFFEDSTRTRISFEAAAKRLSADVINFSAKGSSVSKGESLKDTAQTLEAMGADAVV
                     VRHSASGAPRTLATSGWISAGVVNAGDGTHEHPTQALLDAFTIRKRRYGADSRGRDLS
                     GLRVVIVGDVLHSRVARSNVWLLSTLGADVTLVTPPTLVPQNVSQWPVRVLYDLDQAL
                     AEGPDAVMMLRIQVERMHAAFFPTEREYSRLWGLDALRAATLPESSIVMHPGPMNRGL
                     EISSEAADSPRSTVLEQVTNGVSVRMAVLYLLLAGERNDERGEAK"
     gene            complement(7349..7879)
                     /gene="pyrR"
                     /locus_tag="VN041_12045"
     CDS             complement(7349..7879)
                     /gene="pyrR"
                     /locus_tag="VN041_12045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWU29803.1"
                     /translation="MAARTVLQEADIARALTRIAHEILESNRGAEGLILLGIPTRGVT
                     LADRLGPIIAGIADTEIPVGSIDITLYRDDLSKHPTRAPKRTEIPEGGIDGKTVVLVD
                     DVLFSGRSIRAALDAIQSIGRPSVVRLAILVDRGHRELPIRPDFVGKNIPSSRTERVN
                     VRLKELDGADEVTIES"
     gene            8015..8587
                     /locus_tag="VN041_12050"
     CDS             8015..8587
                     /locus_tag="VN041_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU29804.1"
                     /translation="MGRITFLRHAMPEVESGVGPADWHLSGPGAAAATALHIVTSGST
                     RALSSPELKARQTTALAVGSLLEAIMTDARFREVDREERVHDGFRDARRAWVAGRLDQ
                     RHRGWEAPENAAHRFHEGLLAHAAEHLVVGTHGMVLTAWMVDQGLIAPGDEAVAFWEA
                     LAFPDVIELALPLRRVRAVRLLSPRAAPRR"
     gene            complement(8559..10130)
                     /locus_tag="VN041_12055"
     CDS             complement(8559..10130)
                     /locus_tag="VN041_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWU29805.1"
                     /translation="MRITGLGHAGMFIETVGGNIICDPVLGPSFFGSWFPFPDNRGLD
                     WERYGREADFLYISHRHRDHFDPRLLEKYIRKDIEVLLPEYVTDDLEKDIRALGYENI
                     FYAPAGEIFERGGTKIMITPLRAPSDGPIGDSSLSVDDGTASILNQNDSHPLDLEALL
                     SFGKPEAYFTQVSGAIWWPMVYDLPQDAKQNFARLKREAQNKRAMYYIEKVDAPHVFP
                     MAGPPMFLRDDIFRYNGTGQDDDSIFTDQKEFLAHLKEQAPQYDGQLFIPGTVVDLNG
                     GELNVSQSLYSDAEISHIFDEKWEYLEEQRASRQQELRDEEARRAEIIPPAEMLAAIK
                     EWWEPLIKKSRTIRLGVGGNVRFRIGELDMVVDFPKAKVREYAGEECIYWYTIPADLV
                     STNIVDHEIDWSNSIFLSMQFSVGRSGKFNEFLTTFLKCLSVDRIEYVENWYQEQTDQ
                     TEDAEIGDWVVQRRCPHLRADLTRTGKIDEDGVLTCSMHDWKWDLKTGKCLTTTGHPI
                     RVSQCPLTDEALRAG"
     gene            complement(10223..11311)
                     /locus_tag="VN041_12060"
     CDS             complement(10223..11311)
                     /locus_tag="VN041_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWU29806.1"
                     /translation="MTSHELTLEQARRIVVRAALLDADRPGDVVEVAEQIGQIKIDPT
                     AVIAPCEHTVLWSRIGWSYEPGQLKKAVEQDRLLFEFDGAFRPVSLLPAIRAGMRDPA
                     VRASAIEWLAANDGFRREVLARLRAEGPMTASEIPDTAQVSKAPDGWYGANQVPIMLD
                     LLSRHGEVGIVGRVGRNRIWDVAERAFPQDLPELEPSEATAELERRCLQAEGLARPHR
                     WWSGVGKDAGEAARVEGTSMRFRVDPDALRALDEPDPGGRVAFLNPYDRLLFDRRRLV
                     ELFGFTFVLEQFKPKPQRVYGYFAHPILFGDRFIGMLDAEHDREREVLRVNTIHEFDE
                     WGPDEHELVRLELGELADWLGVPVTGLD"
     gene            complement(11334..14111)
                     /locus_tag="VN041_12065"
     CDS             complement(11334..14111)
                     /locus_tag="VN041_12065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU29807.1"
                     /translation="MQLSVRPLMRSDSTGKWIQGDVTWDGIRRPGIRFDAAQSRWFTD
                     FLAIARDSLLSGTASDWIPLDRVESPLLWQHLHSLPSLGIPMVAAQTHTTVRLADHAS
                     AGIRIDTDEHGGLLVDADIRIDEQPVPAASVRPIGTTGLYRWQVVGADIEVTLAPVHL
                     SAPVRAAITAEAPIEVSAEDRDLFLDEAYPSIARQSTVTAASGLSLPAPVRPVPVLRV
                     NFGSADRLEHRFEWEYAGHGTVPVVSGEPVPADHAFRDADAEQHELRVLERIWASATE
                     TPFEPRAAVIGVPAAEWASTVLPAFEESDVRVVVTGKRKKYRELTGAPEITVSTVEST
                     DPDWFDLGIIVKIDGVSIPFTPLFTALSMRRTKLMLVDGSWFSLAHPALQALRELIDE
                     AADLAEWETGPRISRHQTALWADFEDLADQSESAVSWRALAEGLRDVKNVPPTPVPEA
                     LNAQLRPYQKQGFDWLAFLWGYSLGGVLADDMGLGKTLQLLALIAHAREAGETRPFLV
                     IAPTSVLGTWRSEAARFVPGLRLRIIEGTASRRDERIADAVQQADVVVTSYTLLRLDD
                     SEYADVEWAGVILDEAQFAKNPSTKAHKAIAALRARVKIAVTGTPLENSLTDLWALFS
                     LTAPGLFPSARRFREEYVQPIEKGKVPDNEEGGDYRQRRLERLRRRIRPLMLRRTKEL
                     VAAELPEKQVQEVFVELSPAHRARYDAVLQRERQKLLGLLADLDRNRFIVFRSLTMLR
                     MLALAPGLIDEADAGIPSSKLDVLLEHVQELQAEGHRALVFSQFTSFLDLAAERLTAA
                     GISYVHLDGATRNRAEVIDGFKDGEQPVFLISLKAGGFGLTLTEADYVFLLDPWWNPA
                     AEAQAIDRTHRIGQTRRVFVYRLIASGTIEEKVLALQQRKARLFTAVMDDDELFSKAL
                     TADDIRGLLET"
     gene            14198..14325
                     /locus_tag="VN041_12070"
                     /pseudo
     CDS             14198..14325
                     /locus_tag="VN041_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
BASE COUNT         2107 a         4756 c         5149 g         2313 t
ORIGIN      
        1 cgaacgcgcg cagaccgtcc gcatcctgcg tcagccccca ctgctccagg agcgccgcgt
       61 gcgggtcgat gcccacgcac agcggtccgt gcgcatcaag ggccgcgcgc aggcgggttc
      121 cgaagggagt gctcactcgg cggcctgccg atccagctcg tactcctgca ggctgcgcac
      181 cgagaagccc tcgttcgccg cgtccatgcc gctgaccgcc gcgccgagca ccgccatcgt
      241 ggtgaacagc gccttgtccg cggcgaccgc ggccgcgcgg atctcgtagc cgtcggcacg
      301 ggccgcgcct ccgctgggcg tgttcacgat gatgtcgatc tcgccgtcgt tgatcaagtc
      361 gacgatgttc cgctcgccgt tctcctgcgt ctcgctgtat ttggcggcga gggcgacctg
      421 gatgccgttg cgggacagga tctcggcggt gccctcggtg gccaccagcg agaagcccag
      481 ctgtgcgaga cggtgggcgg gcaggatgac gtcgcgcttg tcggagtcgg caaccgagat
      541 gaacacggtg cccgaggtgg gcatcccgcc gtaggcggct gcctggctct tggcgaacgc
      601 ggtcgggaag tcggccgcga tgcccatcac ctcgccggtg gagcgcatct ccgggccgag
      661 gaccgagtcg acgaccttgc cgtcgtgggt gcggaagcgc ttgaacggca gcaccgcctc
      721 cttcacggcg atcggggcgc ccagcggcac gctcgagccg tcctgtgcgg ggagcatccc
      781 ctccgcgatg agctcggcga tgctcgtccc cgccatcaca cggctcgcag ccttcgccat
      841 cggcacgccc agcgccttcg agacgaacgg caccgtgcgg ctggcccgcg ggttcgcctc
      901 gatgacgtag agcacgccag cgctgatcgc gaactgcacg ttcagcagac cgcggacgcc
      961 cacgccctcg gcgatcgcga gcgtcgccgc ccgaacccgc tcgatctgcg tgcggccgag
     1021 tgagatcggc ggcagggtgc agctcgagtc gccggagtgg atgccggcct cctcgaggtg
     1081 ctccatgatc ccgccgatgt acaggtcggt gccgtcgtac agcgcgtcga cgtccagctc
     1141 gatcgcgtcg tcgaggaagc ggtccacgag caggggcttg ccctcctcga tgacgacgtt
     1201 gccggcggtg cggacgaagt agtcgcgcag ggacgccgtg tcgtagacga tctccatgcc
     1261 gcggccgccg agcacgaagc tcggtcgcac cagcaccggg tagccgatct cctcggcgat
     1321 gcgcaccgcg ccttcgacgt cgatggccgt gccgttgcgc ggcgcgacca ggcccgcgtc
     1381 gtccagcagc cgggagaaca gctcccgctc ctcggcgagg tcgatcgcgg ccgggctggt
     1441 gccgagcacc gtgtagcccg cggcctcgat gcccttggcc aggcccagcg gcgtctggcc
     1501 accgagctgg cacaccacac ccaggatcgt cccgctctgc gcctcggcgt cgagcacctc
     1561 gagcacgtcc tcgagcgtga gcggctcgaa gtacagccgg tccgaggtgt cgtagtcggt
     1621 ggacacggtc tcggggttgc agttgaccat gacggtctcg tacccggcat ccgacagggc
     1681 gaacgacgcg tgcacgcagg agtagtcgaa ctcgacgccc tgcccgatgc ggttcggtcc
     1741 agagccgatg atgaccacct tcgtgcgctc ggacggtgcc acctcggtct cgaagtcgta
     1801 gctcgagtag tggtacggcg tcaacgcagg gaactcgccc gcgcaggtgt ccacggtctt
     1861 gtagaccggg cgcaggccca gggcgtggcg gatgccgcgc acctcgggct cgctgatgcc
     1921 gcggagctgc gcgatctggg catccgagaa gccgtgctcc ttggcgtgcc gcagggtggc
     1981 ggcgtccagc tccgcggcct gtgcgacgct ctgcgcgacc tcgttgatga gcacgatctg
     2041 gtcgaggaac caggggtcca tcgccgtcgc gtcgaacgcc tgctcgatcg tggcgccctt
     2101 gcgcagtgcc tgctgcagca cgacgatccg gccgtcggtg ggggtcttcg cgatctccag
     2161 cagctcttcg accgagcgct cctcggggcc ccagtggaac gacgagccgc gctgttcgag
     2221 ggaccgcagc gccttctgca gcgcggtcgt gtagttgcgg ccgatggcca tggcctcgcc
     2281 caccgacttc atggtggtgg tgagggtggc atccgcggcg gggaacttct cgaacgcgaa
     2341 gcgcggcacc ttgacgacca cgtagtcgag cgtgggctcg aagctggcgg gcgtcacgcc
     2401 ggtgatgtcg ttcgggatct cgtcgagccg gtagccgagc gcgagcttgg cggcgagctt
     2461 ggcgatcggg aagccggtgg ccttcgacgc cagggccgac gagcgcgaga cgcgcgggtt
     2521 catctcgatg acgatgatgc gcccgttggt cggggcgacc gcgaactgga tgttgcagcc
     2581 accggtgtcc acgcccacgg cgcggatgat gtcgatcgag atgtcgcgca tcttctggta
     2641 ctcgcggtcg gtgagcgtca gcgccggcgc gaccgtgatc gagtcgccgg tgtgcacgcc
     2701 gaccgggtcg acgttctcga tggagcagac cacgaccgtg ttgtcggccg tgtcgcgcat
     2761 gagctcgagc tcgtactcct tccagccgag gatcgactcc tccagcagga cctcggtcgt
     2821 gggcgagtcg tgcaggccgg cgccgccgat gcggcgcagg tccgcctcgt cgaacgcgaa
     2881 gccggagccc aggccaccca tggtgaagga cgggcgcacc accagcgggt agccgagctc
     2941 ggccgcaccg gcgagcagct cgtccatggt gtggcagatg accgacttgg cgacgtctgc
     3001 gccggcctcg atcacgagct ccttgaagat ctggcggtcc tcacccttgc ggatggcgtc
     3061 gaccttcgcg ccgatcagct cgacgtcgta cttctccagg atgccgcgct cgtgcagcgc
     3121 catggcggcg ttcagcgcgg tctgcccgcc gagggtcggc aggatcgcgt cgggcttctc
     3181 cttcgcgatg atcgtctcaa tcacctcggg ggtgatcggc tcgatgtacg tggcgtcggc
     3241 gaagtcgggg tcggtcatga tcgtggccgg gttggagttc accaggatga cgcgcacgcc
     3301 ctcctcacgc agcactcggc acgcctgggt gccggagtag tcgaactcgc aggcctggcc
     3361 gatcacgatc ggcccggagc cgatcaccag aacggatgtg atgtcgtcgc gcttcggcat
     3421 tactcagcgt cctttccggc gatgtctgcg aggaccaggt cgcggaacct ggcgaagagg
     3481 tagtgggcgt cgtgggggcc tgcggccgcc tcggggtggt actgcacgga gaaggcgggg
     3541 atgtcgtggg cgcgcagtcc ctcgaccacc tggtcgttca ggccgacatg gctgacctcg
     3601 acggtgccat agccgttggg gctggcgaag gttccgtgca ggggcgcctc gacggcgaat
     3661 ccgtggttgt gggcggtgat ctcgacccgg ccggtggccc ggtcgagcac gggctggttg
     3721 atgccgcgat gaccgaaggt gagcttgtag gtgcccagtc cgagagcgcg cccgaacagc
     3781 tggttgccga agcagatgcc gaagtacggc agcttcgcgt cgagcacctg gcgcagcacg
     3841 gcgacctggt tgtcggatgc ggaggggtct cctgggccgt tggagtagaa caccccgacc
     3901 gggtcgacgg cctggatgtc ggcgaagctc gcggactgcg gcaggacgtg cacgtcgaag
     3961 ccgaggcggg ccaggttgtc gagcgtggac tgcttgacgc ccaggtcgag cacggcgagg
     4021 ttgccgatgc gctccccctc ggcggggatc acggtgggtt cgctcaccga gacctccgcg
     4081 gagaggttct gtccggccat ctgcggggcc tcggtgacgc ggcgcagctg ctcctcggca
     4141 tccagctcgg catccgggcc ggagaagatc ccgccgcgca tggaaccggt gtcgcggatg
     4201 cggcgggtga gcgcgcgcgt gtcgatgccg ctgatgccga cgatgccgtc acgaatcagc
     4261 acgtcgtcga gcgactcctc ggcgcgccag ttggaaacac ggcgcgcggg gtcgcgcacg
     4321 atgtagccgg cgacccagat gcgcgcggac tcgtcgtcct cgcggttcat gccggtgttg
     4381 ccgatgtgcg gcgcggtctg cagcacgatc tgtccggcgt acgacgggtc ggtgagggtc
     4441 tcctggtagc cggtcatgcc ggtggcgaag acgacctcgc ccagggtggt gccgacggcg
     4501 ccgtaggcgc ggccggtgaa gcgggtgccg tcctcgagga cgagcacggc ggacgtcagc
     4561 ggggacggcg ccccggtggt gggattgctc aagactgttc tccgttctcg gagccggcgc
     4621 tcaggcgctg cagctgcggg aggatctgct gcggcccaac ggatgcggga cgcaggaagg
     4681 aatctacgat ggtgccttcg gcgacccgcc agacgaggcg gatcagaccg ttcggctcga
     4741 cgacccggtc gatggcgacc gtcgcgaggt cggctgcgag cagccgttcg gatgcgatga
     4801 acacggtggg agaaccgtcc aggctgagcg ccactccccg gtcggtgagc acgagctcgc
     4861 cacgggcgcg gtacgtcagc gccttcatcg cgatgcgctc cagaggctga tcgtgcacag
     4921 tcgtcgcgac gtacaggact tcgtggcgat cgatgacgtc agcgtgctcc gggactccga
     4981 ggggcgcggt cagcgcggag tcccggctga tgcgtcgtcg ccaggcgaag aacatcgcgg
     5041 cgaggaccag gagtgcgacg gcgatcgtga tcagcactgc tgcttcgcgc gtcatgcgag
     5101 ttcctgcacg agctgcccgt cgtccaccgt cacgacgccg ccgtggaacg tccactgcac
     5161 gcgtccggga agatcacgac cgaggtacgg cgagttgtgg ctgcggccgt gcaggtcggt
     5221 ttcggtgaac acaccgggga cgctcgggtc gtagagggtg acgtgagccg gcgcgccgac
     5281 ctccagcgag ccgaagccct ggagctgtcc gatccccgcg ggtgccgagc tcatgacgcg
     5341 cgcgacgtcg gtccagtcca gcatcccggt ggcgaccatc gactggtgca cgacgcggag
     5401 cgcgctttcc agcccgacca tgccgttggc ggcgacctgc cactcgcagg ccttgttctc
     5461 ggccggatgc ggggcgtggt cggtggcgac gatgtcgatc gtgccgtccg ccaggccctc
     5521 gcggacggcg gtgacgtcct cggcggtgcg cagcggcggg ttgaccttgt accgcgcgtc
     5581 gtagtcgcgg accagctcgt cggtcagcag caggtggtgc ggcgtgacct cggcggtgac
     5641 gcggatgccg cgcttcttgg cccagcggat gatgtcgacc gagcccgcgg tggacaggtg
     5701 gcagacgtgc agccgcgagc cgacgtgctc ggcgagcagc acgtcgcggg cgatgatcga
     5761 ctcctcggcg accgccggcc atccggcgag gccgagctcg gcggagacga tgccctcgtt
     5821 catctgcgcg ccctgggtga ggcgcgggtc ctgggcgtgc tgcgcgatga ctccgtcgaa
     5881 cgccttgacg tactcgagcg cgcgacgcat gatcagcggg tcccagacgc agaacccgtc
     5941 gtcgctgaag acgcgcacgc gagcgcggga gccggccatg gcgccgagct cggcgagccg
     6001 ctcgcccttc tggccgacgg tgacggcgcc gatgggctgc accgtggcgt agccggcggc
     6061 ctcgccgaga gcgagctcct gctcgaccac gccggccgtg tcggcgacgg gctgggtgtt
     6121 cggcatggcg aacacggcgg tgaatccacc ggcggcggcc gcctgggtgc ccgtgagcac
     6181 cgtctcggat gcctcgaacc cgggctcgcg gaggtgcgtg tgcaggtcca ccagtccggg
     6241 cagggcgacg agccccgccg catcgatgac gcgtgcgccg ctgcgggtga gtccggcgcc
     6301 gatctcgacg atgacgccgt cctcgatcgc gatgtcggcg ctgtcaccgc cgagcagctg
     6361 ggctcccgtg atgacgaggt tccgattcac ttcgcttctc ctcgttcgtc gttgcgctcc
     6421 cccgccagca gcaggtagag cacggccatg cgcaccgaga cgccgttagt gacctgctcc
     6481 agcacggtcg agcgcgggga gtctgcggcc tcggaggaga tctccagacc gcggttcatg
     6541 gggccggggt gcatcacgat gctactctcg ggcagcgtcg cggcccgcag tgcgtcgagc
     6601 ccccacagcc gggagtactc gcgctcggtc gggaagaacg ccgcgtgcat gcgctccacc
     6661 tggatgcgga gcatcatcac ggcatccgga ccctcggcca gcgcctggtc caggtcgtag
     6721 agcacgcgca ccggccactg cgagacgttc tgcggtacga gcgtcggcgg ggtgaccagg
     6781 gtcacgtcgg cgccgagggt cgagagcagc cagacattgg agcgtgcgac ccgggagtgc
     6841 agcacatcgc cgacgatgac gacgcgcagc ccgctcaggt cgcggccgcg gctgtcggcg
     6901 ccgtaccggc gcttgcggat cgtgaaggcg tccaggaggg cctgcgtggg gtgctcgtgc
     6961 gtgccgtcgc cggcgttcac gaccccagcc gagatccagc cgctggtggc gagagtgcgg
     7021 ggggctccgg atgccgagtg gcggaccacc acggcatccg cgcccatcgc ttcgagggtc
     7081 tgcgcggtgt ccttgagact ctcgcccttg gagacgctgg atcccttggc ggagaagttg
     7141 atcacgtccg cggagaggcg cttggcagcg gcttcgaagg agatgcgtgt gcgcgtggag
     7201 tcctcgaaga agaggttcac gacggtcttg ccgagcagtg tcgggagctt cttgatctgc
     7261 cgggactggg tctcggccat gtcctcggcc acatcgagga tgcgcagggc ctcgtcgcgc
     7321 tcgaggctgc gggtgtcgag caggtgcctc atgattcgat cgtcacctca tcggcgccgt
     7381 cgagctcctt gaggcggacg ttgacgcgct ccgtgcgcga ggacgggatg ttcttgccga
     7441 cgaagtcggg gcggatcggc agctcgcgat ggccgcggtc cacgaggatc gccagacgca
     7501 cgacggaggg ccgtccgatg gactggatcg catcgagcgc ggcgcggatg ctgcgcccgg
     7561 agaacagcac gtcgtccacc aggacgaccg tcttgccgtc gatgccgccc tcggggatct
     7621 cggtgcgctt gggggcacgg gtcgggtgct tcgacagatc atcccggtac agcgtgatgt
     7681 cgatggagcc gacggggatc tccgtgtcgg cgatgcccgc gatgatgggg ccgagccgat
     7741 cggcgagcgt caccccgcgg gtcggaatgc cgagaaggat cagaccttcg gcgccgcggt
     7801 tggattcgag gatctcatgg gcgatgcgcg tcagcgcgcg cgcgatatcg gcttcttgca
     7861 gcactgttcg tgcggccatc tgccactccc ttctccgcct cacaggacgg gcttaaaggt
     7921 tgggtgatgg ttcaagtcta gcgattcgcc tcgtccgacg ttggtatacc ggcagcagga
     7981 ggcgcctcgg cacgcgcggc ctaagctgag ccgcatgggc cgcatcacct ttctccgcca
     8041 cgcgatgccg gaggtggaat ccggagtggg gccggcggac tggcacctga gcgggccggg
     8101 cgccgcggcg gcaactgcgc tgcacatcgt gaccagcggg tcgacgcgcg cgctgtccag
     8161 cccggagctg aaggcgcggc agaccaccgc gctggcggtg ggctcgctgc ttgaggcgat
     8221 catgaccgac gctcgcttcc gcgaggtcga ccgcgaggag cgcgtgcacg acggattccg
     8281 tgacgcgcga cgggcatggg tcgcggggcg cctcgatcag cggcaccggg gctgggaggc
     8341 gccggagaac gcagcgcacc gattccatga aggtctgctc gcgcatgcgg cggagcacct
     8401 cgtcgtcgga acgcacggga tggtgctgac ggcctggatg gtggatcagg gtctgatcgc
     8461 tccgggcgac gaagccgtcg cgttctggga ggcgctggcc tttccggacg tgatcgagct
     8521 cgccctcccg ctgcggcggg tgcgtgcggt gcgactgctc agcccgcgcg cagcgcctcg
     8581 tcggtgagcg ggcactgcga gactcggatc gggtgaccgg tggtggtcag gcacttgccg
     8641 gtcttcaggt cccacttcca gtcgtgcatg ctgcaggtga gcacgccgtc ctcatcgatc
     8701 ttgccggtgc gcgtcagatc cgcacgcagg tgcgggcagc ggcgctgcac gacccagtcg
     8761 ccgatctcgg cgtcctcggt ctgatcggtc tgctcctggt accagttctc cacgtactcg
     8821 atgcggtcca ccgacaggca cttgaggaac gtggtgagga actcgttgaa cttgccgctg
     8881 cggcccaccg agaactgcat ggagaggaag atcgagttcg accagtcgat ctcgtgatcg
     8941 acgatgttgg tcgagaccag gtcggcgggg atcgtgtacc agtagatgca ctcctccccc
     9001 gcgtactcgc gcaccttcgc cttggggaag tcgacgacca tgtccagttc gccgatcctg
     9061 aaccgcacgt tgccgccgac accgaggcgg atggtgcggg acttcttgat gagcggctcc
     9121 caccactcct tgatcgcggc gagcatctcg gcgggcggga tgatctcggc ccgtcgcgcc
     9181 tcctcgtcgc ggagctcctg ctgccgcgaa gcgcgctgct cctcgaggta ctcccacttc
     9241 tcgtcgaaga tgtggctgat ctcggcatcc gagtacaggc tctgggagac gttcagttcg
     9301 ccgccgttca ggtccacgac ggtgccgggg atgaagagct gtccgtcgta ctgaggtgcc
     9361 tgctccttca ggtgcgcgag gaactccttc tgatccgtga agatcgagtc gtcgtcctgc
     9421 ccggtgccgt tgtagcggaa gatgtcgtcg cgcaggaaca tgggcgggcc cgccatcggg
     9481 aagacgtgcg gggcgtcgac cttctcgatg tagtacatgg cgcgcttgtt ctgcgcctcg
     9541 cgcttcaggc gcgcgaagtt ctgcttggcg tcctgcggaa ggtcgtacac catcggccac
     9601 cagatcgcgc cggagacctg ggtgaagtac gcctcgggct tgccgaagga cagcagcgcc
     9661 tccagatcga gagggtgtga gtcgttctgg ttgaggatgg atgccgtgcc gtcgtcgacg
     9721 ctcagggagg agtcgccgat cggcccgtcg ctgggggcgc gcagcggcgt gatcatgatc
     9781 ttggttccgc cccgctcgaa gatctcgccg gcgggggcgt agaagatgtt ctcgtacccg
     9841 agggcgcgga tgtccttctc gagatcgtcg gtgacgtact cgggcagcag cacctcgatg
     9901 tccttgcgga tgtacttctc cagcaggcgc gggtcgaagt ggtcgcgatg ccggtgcgag
     9961 atgtacagga agtcggcctc gcgaccgtag cgctcccagt cgaggccgcg gttgtccggg
    10021 aacgggaacc aggagccgaa gaaggacggt ccgagcacag ggtcgcagat gatgtttccg
    10081 ccgacagtct cgatgaacat cccggcgtgg ccgagtcccg tgatccgcat gtctctcctc
    10141 gtgcttgggc agcctcgatt ctaccggcgg gctcctgtgc cgcaccggtg accggccggg
    10201 agaaagcgcg ccgggcgcgc cgtcagtcca gcccggtcac cggcacgccc agccaatccg
    10261 ccagctcgcc cagctccagg cgcacgagtt cgtgctcgtc cggtccccac tcgtcgaact
    10321 catggatcgt gttcacgcgc agcacttccc gctcgcgatc gtgctcggca tccagcatcc
    10381 cgatgaagcg gtcgccgaac aggatcggat gcgcgaaata gccgtacacg cgctgcggct
    10441 tcggcttgaa ctgctccagc acgaacgtga acccgaacag ctcgacgagt ctcctccggt
    10501 cgaacagcag gcggtcatag gggttgagga aggctacccg gccgccggga tcgggttcgt
    10561 cgagcgcccg cagcgcgtcg ggatccaccc ggaaccgcat gctggtcccc tcgacccgcg
    10621 cggcctcgcc ggcgtccttc cccacgccgc tccaccagcg gtgcgggcgc gcgagccctt
    10681 ccgcctgcag gcaccggcgc tccagctccg ccgtcgcctc cgacggttcg agctccggca
    10741 ggtcctgcgg gaaggcgcgc tccgcgacat cccagatgcg gttgcgtccg accctgccga
    10801 cgatgcccac ttcgccatga cgggagagca ggtcgagcat gatcggcacc tggttcgcgc
    10861 cgtaccaccc gtcaggcgcc ttcgacacct gtgcggtgtc ggggatctcg gatgccgtca
    10921 tcggtccctc cgcccggagg cgggcgagca cctcgcgccg gaagccgtcg ttcgccgcga
    10981 gccattcgat cgcactcgcc cgcactgccg ggtctcgcat gcccgcccgg atggcgggca
    11041 gcagggagac cggccggaag gcgccgtcga actcgaacag cagccggtcc tgctcgaccg
    11101 ccttcttcag ctgaccgggc tcatacgacc agccgatccg cgaccagagc accgtgtgct
    11161 cgcagggcgc gatcaccgcc gtggggtcga tcttgatctg tccgatctgc tcggcgacct
    11221 cgaccacgtc gccggggcgg tcggcgtcga gcagcgccgc gcgcaccacg atgcgccgtg
    11281 cctgctcgag agtcagctca tgcgaagtca tcgctccgag cctaggccgc cggtcaggtc
    11341 tccagaaggc cccggatgtc gtcggcggtg agcgccttcg agaagagctc gtcgtcgtcc
    11401 atcacggcgg tgaacaggcg cgccttgcgc tgctgcagcg ccaggacctt ctcctcgatc
    11461 gtgcccgagg cgatcagccg gtagacgaac acccggcgcg tctgtccgat gcggtgcgtg
    11521 cggtcgatgg cctgcgcctc ggccgcggga ttccaccagg ggtcgaggag gaacacgtag
    11581 tcggcctcgg tgagcgtgag tccgaatccg cccgccttga ggctgatgag gaacaccggc
    11641 tgctcgccgt ccttgaagcc gtcgatgacc tcggcgcggt ttcgcgttgc cccgtccagg
    11701 tgcacgtagg agattcccgc ggcggtgagc cgctccgcgg cgaggtcgag gaagctcgtg
    11761 aactggctga acaccagcgc tctgtgcccc tccgcctgca gctcctggac gtgctcgagc
    11821 agcacgtcca gcttgctgga ggggattccg gcatccgcct catcgatcag gcccggtgcg
    11881 agcgccagca tccgcagcat ggtcagcgag cggaagacga tgaagcggtt gcggtcgagg
    11941 tcggcgagca ggccgagcag cttctgccgt tcgcgctgca gcacggcgtc gtagcgcgcg
    12001 cggtgggcgg gcgagagctc gacgaacacc tcctgcacct gcttctccgg cagctcggct
    12061 gcgacgagct ccttggtgcg gcgcagcatc agcgggcgga tgcgtcgtcg cagacgctcc
    12121 agccggcgct gccggtagtc gccgccctcc tcgttgtcgg ggaccttgcc cttctcgatg
    12181 ggctgcacgt actcctcgcg gaaccgtcgt gccgacggga acagcccagg ggcggtgagc
    12241 gagaacagcg cccagagatc cgtgaggctg ttctccagcg gagtgccggt cacggcgatc
    12301 ttgacccggg cacgcagcgc cgcgatggcc ttgtgcgcct tcgtcgaggg gttcttcgcg
    12361 aactgcgcct cgtccaggat cacgcctgcc cactcgacat ccgcgtactc gctgtcgtcg
    12421 agacgcagca gcgtatacga ggtgacgacg acgtcggcct gctgtacggc gtcggcgatg
    12481 cgctcgtccc gccgggacgc ggttccctcg atgatccgca accgaaggcc cggcacgaac
    12541 cgcgccgcct cgctgcgcca ggttccgagc acggaggtcg gggcgatcac caggaacggg
    12601 cgcgtctccc ccgcctcacg cgcgtgcgcg atcagtgcga gcagctgcag cgtcttgccg
    12661 agacccatgt catcggcgag cacaccgccg agactgtaac cccacaggaa cgccagccag
    12721 tcgaacccct gcttctggta ggggcgaagc tgcgcgttca gcgcttcagg aaccggcgtg
    12781 ggcggcacgt tcttcacgtc gcggagtccc tcggccagcg cgcgccacga caccgccgac
    12841 tcggactggt ccgcgaggtc ctcgaagtcc gcccacaggg cggtctgatg ccggctgatg
    12901 cgcggaccgg tctcccactc ggcgagatcg gcggcttcgt cgatgagctc gcggagcgcc
    12961 tggagcgcgg ggtgcgcgag ggagaaccag ctgccgtcga cgagcatcag cttggtgcgc
    13021 cgcatgctga gcgcggtgaa caggggcgtg aacgggatcg agacaccgtc gatcttcacg
    13081 atgatgccca gatcgaacca gtccgggtcg gtcgactcga cggtggagac cgtgatctcg
    13141 ggagcgccgg tgagctcacg gtacttcttg cgcttgccgg tgacgacgac gcgcacgtcg
    13201 ctctcctcga aggcgggcag gacggtggac gcccattcgg cggccggcac gccgatcacc
    13261 gccgcccgcg gttcgaacgg ggtttcggtc gcggatgccc agatgcgctc gagcacgcga
    13321 agctcgtgct gctcggcgtc ggcgtcgcgg aaggcgtggt cggcgggcac cggctcgccc
    13381 gagacgaccg gcacggttcc gtggcccgcg tactcccact cgaagcggtg ctcgaggcgg
    13441 tcggccgagc cgaagttgac gcggagcacg ggcacgggcc gcaccggtgc gggcagggac
    13501 aggccgctcg cggctgtgac cgtcgactgc cgcgcgatgc tcgggtacgc ctcgtcgagg
    13561 aacaggtcgc gatcctcggc ggacacctcg atgggcgcct ccgccgtgat cgcggcgcgc
    13621 acgggcgcgc tcaggtgcac gggtgcgagg gtcacctcga tgtcggcccc cacgacctgc
    13681 cagcggtaga ggccggtggt tccgatcggg cggacggatg cggcgggcac aggctgctcg
    13741 tcgatgcgga tgtccgcgtc gaccagcagt ccgccgtgct cgtcggtgtc gatgcggatg
    13801 cccgccgatg catggtcagc gagacggacg gtggtgtgcg tctgcgccgc gaccatcggg
    13861 atgccgaggc tcggcagcga atggaggtgc tgccagagca gcggcgattc gacccggtcc
    13921 agcggtatcc agtccgaggc cgtgccggag agcagcgagt cccgtgcgat ggcgaggaag
    13981 tcggtgaacc agcgcgactg cgcggcatcg aagcggatgc cggggcgccg gatgccgtcc
    14041 caggtgacgt cgccctggat ccacttgccg gtggagtcgc tgcgcatgag cggacgcacg
    14101 gacaactgca cctcgccgtg ctcgcgggtc agctcgcgag gcgtcgcggt gcgagcgggg
    14161 gcggagcccc agcggtcccg cgccctcgac ggacgcacgc gcagcgagcg agctctcccg
    14221 ccaggcgtcg tggctgtacg cccgcgacat cgtcgacggg atcatcgacc accacgacga
    14281 gatcgacgag cagatcacca cgcacagccg tgactggaag ctcga
//
LOCUS       DATMSX010000392        19686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72402,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000392 DATMSX010000000
VERSION     DATMSX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 19686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19686
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72402"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..262
                     /locus_tag="VN041_12075"
     CDS             68..262
                     /locus_tag="VN041_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29808.1"
                     /translation="MNHHRDQAGLTFEQFSERSGLSRQTLLNISAGRYHGDLRTWLKL
                     SSAFETSLDQMLATVWGTDR"
     gene            complement(311..505)
                     /locus_tag="VN041_12080"
     CDS             complement(311..505)
                     /locus_tag="VN041_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29809.1"
                     /translation="MGESSIPQLLTTDDVAVILQVSKRTLESWRTRDEGPRFLYAGKH
                     VRYRPVDVETWIEGNIPVQS"
     gene            935..2251
                     /locus_tag="VN041_12085"
     CDS             935..2251
                     /locus_tag="VN041_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29810.1"
                     /translation="MDRYWSTPRGLHAAQNYRRMVSLRISADMIPPSKRAMGAVKARA
                     REVLGDAFASSRAHASLYPKVRWPKPRAAVALDIALYGELLNAPRVDTICKWLLDELA
                     GLVYRDDRQVKLLFAHADRRDREAELANSRTDPRREDPSASATFGAAWSVISDEPGLR
                     GLDAKPKSSPTLYVHARTRANVLAALRATGYLPYSWDPIDDDDDYKYWNPYTATYPGS
                     RRSLVEYRELLESRIEPDADELTLVGRQLDYHDQASEQRIVDVLFSSLFTFLPAQRFL
                     GWNLLRDRLGTAPYVFDLGVLPDIGGSAAFRSRLQVLLNQRRTQYPELFPMRAKSGIS
                     MVLFEDGTHEKDLDNLVRTVLPDVLDVLRPPRTDRPGWVATAPDIESTYPDIPFLEVA
                     ALSAKDADMPPGTLIFGLSGGHRRQSWWAKAESDLERQLDQLIDRH"
     gene            complement(2327..2878)
                     /locus_tag="VN041_12090"
     CDS             complement(2327..2878)
                     /locus_tag="VN041_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29811.1"
                     /translation="MNRNSEDDSFIETRTYRRVVLLLGATVIICAMSVVLDLKFPPKE
                     MLPTGVLWVLLLTMLAAPIGALFERRYTSAPHDLEIACGVAGGAAAFGVALYASVGWP
                     STGVALAVTAVAAGVGSAGVLVRIHIRRIEDRHQQARDTELSQMRAALDDLRATATPV
                     RLQADGLGSRVRLATKILLRGEA"
     gene            complement(2925..3074)
                     /locus_tag="VN041_12095"
     CDS             complement(2925..3074)
                     /locus_tag="VN041_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29812.1"
                     /translation="MTKAELEERAGRRAPTENERRLAARIIVNGDKARGVKTPQWIID
                     LAKVS"
     gene            complement(3168..3710)
                     /locus_tag="VN041_12100"
     CDS             complement(3168..3710)
                     /locus_tag="VN041_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29813.1"
                     /translation="MSAVAGAITIVTLQLLGIFDIPAWWQSLGVWWRDAGWPWTSAWL
                     VSPGFGGFAAVIAASLAFGGSRHQARLNAWWQRVEWALNLYVKPNATSIEREAGAAAV
                     VELQTTRLARRADRAFLAEVARAVTFDPNGDGLEQDDWADAEARDEAEHDALLDANAE
                     GRADAEREGSSEEDLSPDAR"
     gene            complement(4021..5058)
                     /locus_tag="VN041_12105"
     CDS             complement(4021..5058)
                     /locus_tag="VN041_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29814.1"
                     /translation="MNCECGETAFISAVDYEAQANNCRVPCAHCGTEIYFGPRVAAIR
                     DRNDPALDNVAALAWYHTSTSPDWPSPDYAQQIADSMTGNKRQYWPSREGYLAEQSSK
                     ALHLGTYEAAIENMLRRMENQDDGGSQFYLYRVALSPSRLRINTGYRDENQEIAADLS
                     IGDLDGDDLDAVRYLNVHEALGTLSLAVRPQAIAAVQSIPVPVGGLAAVADSAHVGVE
                     IERVQRAEAGLAKAMAATGAIGHMELREMQLGMRPNPEGVAKQAGKAQNRVYSAWRGL
                     LDRLGECLLPSVSAEIRGDFNDAMRHWQSANQGAEVADFVERYTMMATVLERPRDVVD
                     AVTTQPWRAIA"
     gene            complement(5108..5518)
                     /locus_tag="VN041_12110"
     CDS             complement(5108..5518)
                     /locus_tag="VN041_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29815.1"
                     /translation="MHRTKEQQIKHVVDGLALGVLANGVRAVTSSKMSLEFGFNYAWR
                     RWAHASEFPSIKGHNPGNLFWIGMGKSERRRGARVAWASERWAQPYLTLDGWDVAETL
                     ELHADEVETEDWIELGRLFIERFKEDEIIWAETD"
     gene            complement(5570..6649)
                     /locus_tag="VN041_12115"
     CDS             complement(5570..6649)
                     /locus_tag="VN041_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWU29816.1"
                     /translation="MFVPLTVVSTTEQLASLWLADVDMRAEDAIEQSSREAYHNRVKN
                     NVLPYIGAVPIGSLNAGFIHNLLQRLAPERSYSFASVTRKTLSGMLTFAALNGVIPTS
                     PMNDVPKLKSNTRKRSIQLKEDQLLLIIALIREWEGKNPGRRGGSRPNVRAMEDFLLI
                     TLGTSLRPGEVLGLTLDDVHLLSIPAKVSLSGNVNRTKKFKNIRKDHPKGRGQERTMT
                     VPEYTDAVLRRPVACYEPNPDQLLIPTKNGTPMSVNYIDRLFREFRNQGRELLGRVGI
                     DLDLLTPYALRKTVASVVNQAAGAELAAKALGHTDPRITKKHYIHEMREADPEAAAIL
                     GRAFSRLTPAETAHSVGCPSQESIV"
     gene            complement(6830..7060)
                     /locus_tag="VN041_12120"
     CDS             complement(6830..7060)
                     /locus_tag="VN041_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29817.1"
                     /translation="MSKKNASTKSGSEALAKVALPALLTKEQVAEYFGVSPRTVDSWR
                     YTNTGPTPIKIGKHLRWTADSIRDYIAGQSVA"
     gene            7387..8211
                     /locus_tag="VN041_12125"
     CDS             7387..8211
                     /locus_tag="VN041_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWU29818.1"
                     /translation="MTPVHNDVILPLFEEWLTASFRADGTVRLRMRHVRHLARRVQLT
                     LADEHHLTVALGETRHLAADTRHSILSSWKLFYRWAVARRFVLLDPTLLIEQIPVPVR
                     MPRVAPDEAVQRGLVNATPRDRGLVMLGRYACLRLTEITTLHMRDRRGEVLLIRGKGD
                     KERYVYVNQPLGVALDLLEELLPRGHYFPGETDGHLHPQSVHKIIKRVTGWNPHALRH
                     AGATAAYRGTGDLRAVQEMLGHASLSTTQRYLHLDDDARRRVAAATEIGWSGRLAA"
     gene            complement(8472..8795)
                     /locus_tag="VN041_12130"
     CDS             complement(8472..8795)
                     /locus_tag="VN041_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29819.1"
                     /translation="MTDPTRRARRDALIAAGAIPVDEIEATEEQFAYAVATPNVVVQN
                     PTVRRIAGIVLGVASLLTSAAIVIDGSSPAFDISAITTPAGALVLFLAGAFQVGVTTP
                     NVPRS"
     gene            complement(8805..8972)
                     /locus_tag="VN041_12135"
     CDS             complement(8805..8972)
                     /locus_tag="VN041_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29820.1"
                     /translation="MNEQTEVVVARIDERLVAVERDVKEIKESIRPSWPSITAALVAA
                     SALVITLIDKF"
     gene            complement(8969..9667)
                     /locus_tag="VN041_12140"
     CDS             complement(8969..9667)
                     /locus_tag="VN041_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29821.1"
                     /translation="MSAPTIITIRPGVAFAPPAAASFRRLERALGRQVDVNSTYRDWD
                     LQLRMYNDWRAYVDGTGPRPAHSRAIHPKYSKHCQGLALDSDDWRTPGFIPLAEEHGW
                     IRTAASDPTERHHFEYQWWRDQHRNDPAPAGHTIPTTQEDEEDDMASLKGATYVRAAD
                     KANVCILFNEASGFYVEHTGVGGKYNNPIAENWGTGTWPPITESHATVIKRALDAVRR
                     TTVAGSLSVDLDEQ"
     gene            complement(9664..11280)
                     /locus_tag="VN041_12145"
     CDS             complement(9664..11280)
                     /locus_tag="VN041_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29822.1"
                     /translation="MTVTEHYAHAVSDALPGVQLGVEGGQVGLDVARWPHVQATIKTP
                     ARAGLYAAMDPRAAAHRVVLSCGEQVMGLHRQFDLGIRHVGIGADGLLEMQLASDEAI
                     VEDETPDADVRPWAYLRANGYVAEVLTEMVLARSGFNLTDPGPVADITPYWSLTNRVV
                     NPRFQTTIKGAAAGSGTSGLVSTTHTAAGSTARRALQFTCGTAGGSAIVAGWDPAAGH
                     ESALPVSAGDWVSASFDASCVAPNRDRKIRVQFFDGAFTLIYEVIPGGQPTAPDTRWT
                     THAALVQAPIGAVYCAVRAFLGPIASGADQRVFVTNVSVVQWDDYVPAFNGGDTPPGG
                     YTVSWDGAPDDSTSTRYPIVPLDPESLVQKAGQGGMSFLAPVLQACGLRLVCDETRAW
                     SLRGIDHVEPGELTIAYGETMTDFALGAGLDGWCDAATVHYEWERYGVTYEMDDTFEL
                     VTPPRKRIRRTLNMPYPGPGRAENIVRRAQAVGGRSSTARAVSNWSVKSEQTLTVTTD
                     DTTLTGQVGAVTFDLDTDEMTVTIREEPPA"
     gene            complement(11277..11633)
                     /locus_tag="VN041_12150"
     CDS             complement(11277..11633)
                     /locus_tag="VN041_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29823.1"
                     /translation="MTTITHSTGTIVPTIVHGFAARREARTLIHPVLGSPDDDVSLRP
                     AGRRSGVLELVFSTSAAAVAAEAVFAIGQVLTITDPDVTAVGMSFVVAGGTVGVELSE
                     SRRSWIVTVPFKELAP"
     gene            complement(11633..12925)
                     /locus_tag="VN041_12155"
     CDS             complement(11633..12925)
                     /locus_tag="VN041_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29824.1"
                     /translation="MAGGYQVGIGSETKAFKQGIDTGVIAPLEDAQKELLELGRNRGP
                     EQLEKQLREDQKATEKLGDETKRTANAIEREYRDSYRKMKNASDDALDDGAKGMSRLR
                     EGAGEVTQEIGQNLGEAVSSVRGNLADLGQVGQDTLGGLAATLAGTGGAAGILGAAAL
                     AAGATGLGLVTAELEKQNDRTQKLRQYFSEAWKAAVDGGRDYIDTATLVSQINEIALG
                     EDFAEQRKNALEVSKRTGLDLQTIYRALAGDAASLEQVHGRLTESEREYWDTLKAVGE
                     GSKTVTTEQADAYRDQHNAVSALLGDLDGVKSANADAADQARIAARAVSEQLLATIAG
                     AESATQAVDDFGNTLITLPDGQEIVIDAKTGQATSDVSRFKKDTDEAITQLNRRAISL
                     VVKTALRDAQNQVNGFIAANDGKSFRLRGRITVDDGWD"
     gene            complement(12928..13422)
                     /locus_tag="VN041_12160"
     CDS             complement(12928..13422)
                     /locus_tag="VN041_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29825.1"
                     /translation="MRLSLLVSRDFAVLVQAVRSLPAEVSKQYRAAARRVLEPAFKSE
                     MTERAETHLQQRVIVDTARVSIRDDNAILRSATIGKIGDGVPASVLAGGAEFGNDPNR
                     TVVSRSRKGKEYKRRMGGVFRRPQRGGYVFFPAVQAFIPRAGALLWQTAYRTVAETFE
                     KVSK"
     gene            complement(13426..13848)
                     /locus_tag="VN041_12165"
     CDS             complement(13426..13848)
                     /locus_tag="VN041_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29826.1"
                     /translation="MVAIAVKPFILRDCTLNIKDGATDVGDYEAHVSRVEITPTVPTQ
                     TWKGLTPTAVFQDVGAAEWVANIDYAQDYETANSFAQYLLANPGKQVTATFTPKKGTG
                     AKKITATVTLLPGGIGGGVGAFATSSVALPVTGQPVLA"
     gene            complement(13855..14247)
                     /locus_tag="VN041_12170"
     CDS             complement(13855..14247)
                     /locus_tag="VN041_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29827.1"
                     /translation="MSSDLRAWFSAQLKPLLPKRPDGSPAWRYIPNQDTPAAITVPTL
                     VFKLLELDPLPEAPLGALRARIVLTLLSPHEDDVKAENTLDNQVIELVTALDPHSTIS
                     WETARKVRDAKTDRLGWDFTVTAVINKE"
     gene            complement(14237..14866)
                     /locus_tag="VN041_12175"
     CDS             complement(14237..14866)
                     /locus_tag="VN041_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29828.1"
                     /translation="MSTWFTATDETRLLAAWSDAPIENLEVCGMILEVAREQVIAYAP
                     ADTPAVYEPATLTWPLGSTATLTREGDIVTADVTIADPDEEARLPLPDLFGPIGTVYQ
                     SIVDRAVQVSIVEVAEFGALGINVTATGLEPITGPGTFKWVAGPADGDGVPTRYVYAQ
                     LQQAKNLLTAGTVSQAGNLGEGEFVFQPRPLDRTIKQIIRPVDGKPHVL"
     gene            complement(14888..16567)
                     /locus_tag="VN041_12180"
     CDS             complement(14888..16567)
                     /locus_tag="VN041_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29829.1"
                     /translation="MHDITIQGGEILADLDDRSMTGLLIPFNEEGRTNAGRFTVQAGS
                     IELPADPSVVTLNIDHDRYQPVGRATRLWETARGIMATFSIGRTPAGDAALADTSRRS
                     LSAEFKTDIDAQRIARNGVLAAAAQVVAGAFPSARVLAELADDSAADYQDRAETIFTD
                     EDGRKYKRVYESSSTTEDVEGGTQTTTVTTSTETEITSADTEKEPTVAEQETVQAGAV
                     PGTIAPNGGGSTAVLASRGPSGQEIASAIAAVKNNRADRQAVEVLAALQDIVISDAGA
                     AIQPTWMGDIAAGITYVQEYITLFNPGTEISIGGKKGYKVHRGTAAAPLDAPMDGTWA
                     GNKTAIKSNKGFVTEHSSVRDNWAMGEDIGREFWDLSGGLEFVLAFLRMQQEDYLIWQ
                     DDLALSYAVAAAGAPIAPNSPALPDNYPTALKHLIQGILAVKKRKKDGRRDTPTFAIV
                     NDIDFEELIYAAGGEQNLPAFVNIALSTAGTGTVDGNVQIVNGDIGIEDTGAALVGAS
                     YAIDFDRPAGGLLEVDALDLARGGIDKAVHGYLQTFVKRPEALALIGVADA"
     gene            complement(16570..17676)
                     /locus_tag="VN041_12185"
     CDS             complement(16570..17676)
                     /locus_tag="VN041_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29830.1"
                     /translation="MGFFRDLFLGPAASAVTGGLLSWYSPQDSIHSMFVADALPVAPD
                     AITRDTAFRVPAVDRAHDVICSVIADMPWVAYDGTRMVEDPDTGELVPTRLEKQPEWL
                     TNTKTGIPPYDLRWAVASDLFMSGWAAIGFELGTDRLPVDALHIPRALWNIGDDGTIT
                     VNARIDARYRQRVVPIRLGYGKNGMLVDALNDIKDARTIADAYRDRIDNPIAQTILTV
                     DKDIWDGWGWDEREEFRQKWIAARSAAGGAVAMKPTYVDVDVSGAIPTDLFETGRNAN
                     RLDLANHAGLPASMLEGVRQGGSGGGTEMRYTGVQNGAQRSEVWDFGVRKYAGAIGAR
                     MSLDDVCAPGHLIRLDTSRFLAVPTPPEQQTSED"
     gene            complement(17699..17932)
                     /locus_tag="VN041_12190"
     CDS             complement(17699..17932)
                     /locus_tag="VN041_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29831.1"
                     /translation="MTRPSIRLEKSAGGVTISCTACPHWHAFAATMGGAHQSASGHEA
                     RAHRGEYRARNAAKMYARRHPEDADTPTRIMNV"
     gene            complement(17943..19547)
                     /locus_tag="VN041_12195"
     CDS             complement(17943..19547)
                     /locus_tag="VN041_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29832.1"
                     /translation="MSATPTLADLWDEGNWLEWRSRINHLDPIRLDALVTTEQSRGEF
                     IQGARLLRLDERQRAGDLGWGPSPMQLMVADLLAAGRFMNVIFEPRRSTKTTAVQAVM
                     MGRCWSREDYTVGWTMFTTGMKAGQRFREDIVKHLDKLWPDRRKSPITIGLSKGGEQL
                     EFRDTGALLRVGTPNGDMFRSGGFDFAFGDEAGEADVEQGDEVNRAVIPTMDTKPGSQ
                     FVLAGTGAKYRTGQLLWENLHDEDAAVLWHGIPENIDRAQLVSWEPDIPHPKTGATGG
                     RMREWIEATHPGLGFTTLTDAPKRSFDKFSNLDDFLIEYGGQFGFEGAADVAIPPAML
                     ERALRKGVPPEPPARFAAALKVHHLGHAASLAVAWEHEEDGDLVTAATGEVIRKRAVA
                     LWWWQQGTTGLSREVLVRLRRRPTTLWYDKRGYTEQIAEHELAKASPRPRLEPTKPAD
                     IPGSAVGLMKALEDDDLIIFATPALEKALRIATRQAFGNYGTWRFGAPKDDPEADVTP
                     LEAAALALRFLADAPTHVNPADAFHF"
BASE COUNT         3106 a         6499 c         6448 g         3633 t
ORIGIN      
        1 cccccccccc cccccccccc ccgacgggcc gcttcagcat cggacccgaa gccaacttcg
       61 cgtcatcctg aaccatcacc gcgaccaggc tggcctgaca ttcgagcaat tctctgagcg
      121 atcggggcta agccgacaga ccctcttgaa catctcagcc ggccgatacc acggcgacct
      181 ccgcacatgg ctgaagttgt cgagcgcttt cgagacgtcg ctcgatcaga tgctcgccac
      241 ggtctgggga accgaccgtt gaggctaagg gcgctagttc ttcggtatca gaccacgcgc
      301 tcgagcatcc tcaagactgt acgggaatgt ttccctcgat ccaggtctcg acgtcgaccg
      361 gacgataccg cacgtgtttg ccggcgtaga ggaatcgggg cccttcgtcg cgcgtacgcc
      421 atgattcgag tgtccgtttt gagacctgga ggatgacagc cacgtcgtcg gtcgtcagca
      481 actgcgggat agatgactca cccatgcgtt acagattgcc accaacaagc agacgatgcg
      541 tagtttccag tagcaaattt tcgaggacgc tccgaaagct gggaaaacgg acagaaaacg
      601 gacacagccg tccaaaatca gggccttcat aggaccttca ttgggccgtt ttgccgcgga
      661 atcacgcgga agttgacgaa tctcgctcga ccttgaatca taagcctcac gaagtgggat
      721 caatacgaag gtcattatga atcaacctcg cgtagttcgt ctgcagctgg tcctgggcgg
      781 gtggactacc agatcgggac acagagtcat gaccattgct acgcggtagg tgcgctcacg
      841 gagttcgcgt ccaatgcggc cccgtattct gacgatgggc gctgttggag cgctccagta
      901 ggcgaccgcg gtcgaccatc catgggagga ctagatggat cgttactggt cgacgccgcg
      961 cggactgcac gccgcccaga actaccgcag aatggtctcg cttcgcatca gcgcagacat
     1021 gattcctccg agcaaacggg cgatgggcgc tgtgaaggcc cgcgcccggg aagtgctcgg
     1081 cgatgccttc gcctcgtcca gagcacatgc ttccctgtac ccgaaggttc gttggcccaa
     1141 gccccgcgcc gcggtggcgc tcgatatcgc actgtacggc gaactcttga acgcacctcg
     1201 cgtcgacacc atctgcaagt ggcttctcga cgagcttgcg ggccttgtct atcgcgacga
     1261 cagacaggtc aagttgctgt tcgctcacgc ggacaggcgg gaccgagagg cggaactggc
     1321 caacagccga acggatccgc gacgagaaga cccgtcagca tcagcgacgt tcggggcggc
     1381 ctggagcgtg atctcggacg agcccggcct gcggggcctg gatgcgaagc ctaagtcgtc
     1441 gccgacgttg tacgtgcatg ccagaacccg cgcaaatgtg ctggctgcgc taagggcgac
     1501 aggctacctc ccctattcat gggatcccat agacgatgac gacgactaca agtactggaa
     1561 tccctacacg gcgacctatc cgggttctcg tagaagcctt gtcgagtata gggagctgct
     1621 ggaatcgcgc atcgagcccg acgcagacga gctcaccctc gttggacggc agttggacta
     1681 tcacgatcaa gcttctgaac agcggatcgt tgatgtcttg ttctcctcgc ttttcacctt
     1741 ccttcctgcg cagcggttcc tcggctggaa tctcctacgc gatcgcctgg gcacggcgcc
     1801 ctatgtgttc gatcttggcg tgctacccga catcggtggc agtgccgcgt tcaggtcgcg
     1861 cctgcaagtg ctcttgaacc agcgtcgaac tcaataccca gagctgttcc cgatgcgcgc
     1921 aaagagtggg atatctatgg tcttgttcga agacggcacc catgagaagg atctcgacaa
     1981 ccttgttcgt acggtgctac ccgacgttct cgatgtcctg cgtcccccga ggacggaccg
     2041 cccagggtgg gtggccaccg cgcccgacat cgaatccacc tatccagaca tcccctttct
     2101 cgaggtggct gcgctttcgg cgaaggacgc tgacatgcca ccgggcacgc tgatcttcgg
     2161 cctttcaggg ggccatcggc gccaaagctg gtgggcaaaa gcggagtcgg acttggagag
     2221 gcagctcgac caactcatcg accggcacta ggacgttccc gcgctctttg cgaccggcgt
     2281 cacacgaaag gcaatctgat gttggacggc ccttcagcgg tctgatctac gcttcgccgc
     2341 gcagaaggat tttcgtggca agacggacac gtgatccaag accatcagcc tgaagtcgga
     2401 cgggcgtcgc cgtagcgcgg aggtcatcaa gcgcagcccg catctgactg agctcggtgt
     2461 cgcgggcctg ttggtggcgg tcttcgatgc gtcgaatgtg aatccggacg agcactcccg
     2521 ccgatccgac cccggcggcg acagccgtca ccgccagagc cacccccgtt gatggccatc
     2581 caacggacgc gtacaacgcg accccgaatg cggctgcgcc acccgctaca ccgcacgcga
     2641 tttcaagatc atgcggtgcg gacgtgtacc ggcgctcgaa aagagcaccg atcggcgcag
     2701 ccaacatcgt tagcagcaga acccaaagga ctcctgtcgg aagcatctcc ttcggcggga
     2761 acttcaggtc caacacgacg ctcatcgcgc agatgatgac ggtggcgcca agaagaagta
     2821 cgactcgcct gtaagtgcgc gtctcgatga agctgtcgtc ttcggagttc ctgttcatgc
     2881 gggtactgtc ccacaagagc accagtgccg aagggatcag ccgctcagga aactttagcg
     2941 aggtcgataa tccactgcgg ggtcttcact cctcgcgcct tgtcaccgtt gacgatgatg
     3001 cgcgcggcga gacgacgctc gttctccgtt ggggcacgtc gacctgctcg ctcttcgagt
     3061 tctgccttcg tcatctcaca cctccttcgc gatcggtgtc tgcctgcgtt cagtgtaatg
     3121 ctcacggtcc aatggaagct agaagacagc cgtggagatc tacgctctca gcgagcgtct
     3181 ggactcaagt cctcctcgga cgagccttcc cgttctgcat cagctcgtcc ttctgcgttc
     3241 gcatcgagaa gagcgtcgtg ctcggcttcg tcgcgtgcct cggcatccgc ccagtcatct
     3301 tgttccaagc cgtcgccgtt cggatcgaat gtcacggccc gcgctacttc agcaaggaac
     3361 gcgcggtccg cacgtcgcgc gaggcgcgtt gtctgcagtt cgacgacagc agcggcccca
     3421 gcttctcgtt caatgctcgt cgcattcggc ttgacgtaca ggttcaaggc ccactccaca
     3481 cgttgccacc atgcgttcag tcgcgcctgg tgccgcgagc ctccgaacgc gagggatgcc
     3541 gcaatcacgg cggcgaaccc tccgaatcct ggactgacca gccacgcact cgtccagggc
     3601 cagcctgcat cgcgccacca cacgcccagg ctttgccacc atgctgggat atcaaagatg
     3661 ccgagcagct gaagagtaac gatagtaatc gccccggcaa cggcgctcat ggcagacgcg
     3721 aatggcgccc ggtgcgtgga tatccaggct ttcactgtcg cacctcatca gggttcgggt
     3781 ggtgtggcct catagcgggg ggagtctaag gtccaagatc cccacggggt ccaggtccgc
     3841 ggatacgttc cgggtctggt cacgttggcg catgtactca ccgtaatcgg tgttcctcgc
     3901 cctaattttt gctctgtgcg caacggccca tccgagccgc ccgacgcccg tcgcgatcgc
     3961 gcactccgta tggcgtcttc gcactcaccc gccatcgcta tcgtcccata tgaacctggc
     4021 ctacgcgatg gctctccatg gttgtgtcgt aaccgcgtcg acgacatctc gcggacgttc
     4081 gaggacggtc gccatcatcg tgtaccgctc cacgaagtcg gcgacctcgg caccctggtt
     4141 ggcgctttgc cagtgccgca tcgcgtcgtt gaagtcgcca cggatctccg cggagacgct
     4201 cggaaggagg cattccccta gccgatcgag gagcccacgc caggcgctgt agacacggtt
     4261 ctgcgcctta cccgcttgtt tcgcgacccc ttccgggttc ggacgcatcc ccagctgcat
     4321 ctcccgcagt tccatgtgtc cgatcgctcc ggtagctgcc atggccttcg ctagtcctgc
     4381 ctccgcgcgc tgcaccctct cgatttccac ccccacgtgc gcgctgtcgg caaccgcggc
     4441 cagccctcct accggcacgg ggatcgactg aactgccgca atcgcctgcg gacgtaccgc
     4501 cagggacagc gtccccagcg cctcgtgcac gttgagatac cggacggcat cgagatcgtc
     4561 tccgtcgagg tcaccgatac tgagatcggc tgcgatctct tggttctcgt ctctgtaccc
     4621 ggtgttgatc cgcagccggc tggggctcag cgcaacccgg tagaggtaga actgcgaacc
     4681 gccgtcgtct tggttctcca tccggcggag catgttctcg atcgctgcct cgtacgtgcc
     4741 gagatgcagc gccttcgacg attgctcggc gagatagcct tcgcgagagg gccagtattg
     4801 tcgcttgttc ccggtcatgg agtcggcgat ctgctgcgcg tagtccggcg atggccaatc
     4861 aggtgaagtg ctcgtgtggt accaagcgag ggccgcgacg ttgtcgaggg ctggatcatt
     4921 ccggtcgcgg atcgcagcaa ctcgcgggcc gaagtagatc tccgtgccac agtgcgcaca
     4981 cggcactcgg cagttgttgg cttgcgcctc atagtcaacc gccgaaatga aagcggtctc
     5041 tccgcattcg cagttcatcc aatagccgta gttgaaccca tcgaatcgtt caacaagtgc
     5101 ggggcgttca atcggtctcc gcccagatga tctcgtcttc tttgaatcgt tcgatgaaga
     5161 gccggcccag ttcaatccaa tcctccgtct ccacttcgtc ggcatggagc tcaagcgtct
     5221 ccgccacgtc ccagccatcg agagtgaggt aaggctgtgc ccatcgctcg gatgcccagg
     5281 cgactcgagc tccacgtcgt cgttcagatt tccccattcc gatccagaac aggtttcccg
     5341 ggttgtgccc cttgatgctc gggaactcgg atgcgtgtgc ccaccggcgc catgcgtagt
     5401 tgaacccgaa ctccagcgac atcttgctgc tcgtcactgc tcggacgccg ttggcgagca
     5461 ctccgagggc gagcccgtcg acgacgtgtt tgatctgctg ttctttcgtt ctgtgcacat
     5521 gttcacgcta ccgaccggtt ccgacgtcac ggagtccgta gcgggccgct cagacgatac
     5581 tctcctggct cggacaaccc accgagtgag cggtttctgc cggggtgagc cgggagaacg
     5641 cccggccgag gatcgcggcg gcctcggggt ccgcttccct catctcgtgg atgtagtgct
     5701 tcttcgtgat ccgcgggtcc gtgtgcccga gggccttcgc ggctagttcg gcaccagctg
     5761 cctggttgac gaccgatgcc acggtcttcc tcagcgcgta cggcgtcagt aggtcgaggt
     5821 caatgcccac acgccctagc agttcacgac cctgattgcg aaactcgcgg aagaggcggt
     5881 cgatgtagtt gacggacatc ggtgtgccgt tcttggtcgg tatgaggagc tgatcgggat
     5941 tcggctcgta gcacgcgaca ggccgtctga gcaccgcgtc cgtgtactcg ggcacggtca
     6001 tcgtgcgctc ctgccccctg cctttggggt ggtctttccg gatgttcttg aacttcttcg
     6061 tcctgttgac gttgcccgag agcgagacct tcgccggtat cgacagcagg tgtacgtcgt
     6121 cgagagtcag accgagcacc tcgccaggcc gtaacgatgt acccagggtg atgagcagga
     6181 agtcctccat ggcgcggacg ttgggcctgc ttccacctcg tcggcccgga ttcttgccct
     6241 cccactctcg gatcagggcg atgatgagca gaagctggtc ttccttgagt tgaatgcttc
     6301 gcttccgcgt attgctcttc aacttgggaa cgtcgttcat cgggctcgtc gggatcacgc
     6361 cgttgagtgc ggcgaaggtg agcattcccg atagcgtctt tcgcgttaca gaggcgaagc
     6421 tgtatgaccg ctcaggggcg aggcgctgca gaaggttgtg tatgaagccg gcattgagtg
     6481 atccgatcgg cacagcgccg atgtagggaa gcacgttgtt cttgacgcgg ttgtgatatg
     6541 cctccctact ggactgctcg atcgcgtcct ctgctctcat gtcgacgtca gcgagccaga
     6601 gcgatgcgag ctgttcggtg gtggacacga ccgtcagcgg cacgaacatg ccgccccaca
     6661 cctggttcgc cttgatctgg atcgcgtctt cggcttcctc cgcagtgttg ccccacgcct
     6721 cgagaacgtg aaggtcgccg acaccgtcgc gaactcggcc cctgctccga taggcaccac
     6781 cgatctcatt ggtggaaacc ttgccgagct cacccggctt cttctcggcc taggcaacgc
     6841 tctgccctgc gatgtagtcc cggatcgaat ccgcggtcca tcggaggtgc ttgccgatct
     6901 tgatgggtgt cggtccagtg ttcgtgtagc gccacgaatc gaccgtccgc ggcgacactc
     6961 cgaagtactc agcgacctgc tccttcgtca gaagcgccgg gagcgccacc ttggcgagag
     7021 cttcggatcc tgacttggtt gacgcgttct tcttgctcat gacggtcaca tacccggtgc
     7081 gatccggatt ctgcccggat cgtcgcgaac catcacgtag tcgttgcacc gaagccccat
     7141 cagaaggccg tggtaggcgg tttctggtga gttctcggtg agtgacaccg tgcgtaccct
     7201 cgttggcata cataggagaa ggcgccccat cccttgaaaa cactgggatg tgacgccttc
     7261 tattggctcc ccggcttgga ctcgaaccaa gaacctgccg gtttcaagcc cgtcgtctcg
     7321 ctggacgccc accgcgccgc gcgccagacc aagacggcct cctagccacg cgggtagata
     7381 ggagggatga cacctgtgca caacgatgtg atcctgcccc tgttcgaaga gtggctgacc
     7441 gcgagtttcc gcgcggacgg caccgtacgg ctgcggatgc gccacgtccg gcacctcgcc
     7501 cggcgagtgc agctcacgct cgccgatgag catcatctga ccgtcgcgct cggcgagacc
     7561 cggcacctcg ccgccgacac ccgccactcg atcttgtcga gttggaagct gttctaccgg
     7621 tgggcggtcg cgcgtcggtt cgtgctgctc gatccgacgc tgctcatcga gcagatacca
     7681 gtcccggtcc ggatgccgcg cgttgcaccc gacgaggctg tgcaacgcgg cctggtgaac
     7741 gccaccccac gagatcgagg gctcgtcatg ctcggccgct acgcgtgcct gcggctcacc
     7801 gagatcacga cgctgcacat gcgcgaccgc cgcggcgagg tgctgctgat ccgcggcaag
     7861 ggcgacaagg aacgctacgt ctacgtgaat cagccgctcg gagtcgccct cgacctgctc
     7921 gaggaactgc tgccccgcgg ccattacttc cccggggaga cagacgggca cctgcacccg
     7981 caatcggtgc acaagattat caagcgcgtg accggctgga acccacacgc gctgagacac
     8041 gcgggcgcga cggccgcata tcgcgggacc ggcgacctgc gagcggtgca ggagatgctc
     8101 ggtcacgctt cgctgtcgac cacacagcga tacctccacc tcgacgacga cgcacgccgt
     8161 cgagtcgcgg ccgcgaccga aatcgggtgg agcggtaggc tcgcggcatg agcgaccgat
     8221 cgaagttcga catttcacca aagcgcaccg cctcgccgat gggcgcgacc agtgccgagg
     8281 tggtgtgctg gacgatcttg ggcgtggcac tccccgtgct tctgatcgcg gtgatcatgt
     8341 acttcaccct cagcggaagc tactcggcct cggaaagctc gaaagcagca gcgctcggtt
     8401 gggtgacgtt ctcccaatgg gcggtcggga tcaccctcgt tccggcgctg ctgctgacgg
     8461 gcggacggca gttagctgcg gggtacgttc ggggtcgtga cgccgacctg gaacgcgccg
     8521 gcgaggaaca gtacgagggc gccggcgggc gtggtgatcg cgctgatgtc gaaggcggga
     8581 ctgctcccgt cgatgacgat cgcggctgag gtcagcagcg acgcgacacc gagaacgatg
     8641 ccggcgatgc ggcgcacggt cgggttctgc acgacgacgt tcggggtcgc gacggcgtag
     8701 gcgaactgct cctcggtcgc ctcgatctcg tcgacgggga tcgcgccggc ggcgatgagt
     8761 gcatcgcggc gggctctgcg ggtggggtca gtcatggttt gctcctagaa cttgtcgatg
     8821 agggtgatga cgagggcgct cgcggcgacg agcgcggcgg tgatcgatgg ccaggacggg
     8881 cggatgctct ccttgatctc cttcacgtcg cgctcgacgg cgacgagccg ctcgtcgatg
     8941 cgagcgacga cgacctcggt ctgctcgttc actgttcgtc gagatcgacg ctgagggatc
     9001 cggcgacggt ggtgcgccgg acggcgtcga gcgcgcgctt gatcactgtc gcgtgggatt
     9061 cggtgatcgg tggccaggtg ccggtgcccc agttctctgc gatggggttg ttgtacttgc
     9121 ctccgacgcc ggtgtgctca acgtagaagc cggatgcttc gttgaacaga atgcacacgt
     9181 tggccttgtc ggctgcgcgc acgtaggtcg cgcctttgag tgatgccatg tcgtcttcct
     9241 cgtcttcctg ggtggtgggg atggtgtgcc cggcgggtgc cgggtcgttg cggtgctggt
     9301 cgcgccacca ctggtattcg aagtggtggc gttcggtggg gtcggatgcg gcggtgcgga
     9361 tccagccgtg ctcttcggcg agggggatga atcccggtgt gcgccagtcg tccgagtcga
     9421 gggcgagccc ctggcagtgt ttcgagtact tcgggtggat cgcacgcgag tgcgccggcc
     9481 gcgggcctgt gccgtccacg taggcgcgcc agtcgttgta catccgcagc tgaagatccc
     9541 agtcacggta agtgctgttg acgtctacct gccgaccgag cgcccgctcg aggcgccgaa
     9601 acgatgcagc cgccggcggg gcgaacgcga caccggggcg gatcgtgatg atggtgggcg
     9661 cgctcatgcg ggtggttcct ctcggatggt gacggtcatc tcgtctgtgt cgaggtcgaa
     9721 ggtgacggcg ccgacttgcc cggtgagggt ggtgtcgtcg gtcgtgactg tcagggtctg
     9781 ctcgctcttg accgaccagt tcgatacggc tctcgcagtg gacgagcgcc cgccgacagc
     9841 ctgtgcccgg cggacgatat tctcggcgcg gccggggccg ggatagggca tgttgagtgt
     9901 tcggcggatg cgcttgcgcg gcggggtgac gagctcgaac gtgtcgtcca tctcgtaggt
     9961 gacgccgtag cgttcccatt cgtagtgcac ggtcgccgcg tcgcaccatc cgtcgaggcc
    10021 ggcaccgagg gcgaagtcgg tcatcgtctc gccgtacgcg atcgtcagct cacccggctc
    10081 gacgtgatcg attccgcgga gcgaccaggc gcgggtctcg tcgcacacga gtcgcagacc
    10141 gcacgcttgc agcaccgggg cgaggaagct catgccgccc tgcccggcct tctgaacgag
    10201 ggactccggg tcgagcggca cgatcgggta gcgggtcgag gtggagtcat cgggcgcacc
    10261 gtcccagctg accgtgtacc cgccgggcgg ggtgtcgcca ccgttgaacg cgggcacgta
    10321 gtcgtcccac tggacgacac tcacgttcgt gacgaacaca cgctgatcgg cgcccgatgc
    10381 gatcggcccg aggaacgcgc gaacggcgca gtagacggcg ccgatgggtg cttgcaccag
    10441 tgccgcgtgc gtcgtccagc gggtgtccgg ggcggtcggt tgaccgcccg ggatgacctc
    10501 gtagatcagg gtgaacgcgc cgtcgaagaa ctggacccgt atcttgcggt cccggttcgg
    10561 ggcgacacag gacgcgtcga aggacgcgct cacccagtcg ccggcgctca ccggcagcgc
    10621 ggactcatgc ccggcggccg gatcccatcc ggcgacgatg gcactgccac cggccgtccc
    10681 gcaggtgaac tgcagcgcac gccgcgcggt cgacccggcc gcggtatgcg tagtggagac
    10741 gagccccgat gtgccagagc ccgcggccgc gcccttgatg gtcgtctgga accgcggatt
    10801 gacgacacgg ttcgtgagcg accaatacgg ggtgatatcg gccaccgggc cgggatcggt
    10861 gagattaaac ccagaccggg ccagcaccat ctcggtcagc acctcggcga cgtacccgtt
    10921 ggcgcgcaga tacgcccacg ggcgcacatc ggcatccggg gtctcatcct ccacgatcgc
    10981 ctcgtcggat gcgagctgca tctcgagcag accatccgca ccgatcccga cgtgccggat
    11041 gccgagatcg aactgccggt gcaggcccat cacctgttca ccacacgaca gcaccacccg
    11101 gtgagccgcc gcccgcgggt ccatcgcggc gtacagaccg gcacgggccg gcgtcttgat
    11161 ggtcgcctgc acgtggggcc acctcgcaac atcgaggccg acctgaccgc cctcgacacc
    11221 gagctgcacg cccggaagcg cgtccgacac ggcgtgcgcg tagtgctccg tcaccgtcat
    11281 ggcgcgagct ccttgaacgg gactgtcacg atccacgagc ggcgggactc tgacagctcg
    11341 accccgactg tgccaccagc gacgacgaac gacataccga ccgccgtgac atccggatca
    11401 gtgatcgtga gcacctgccc gatcgcgaac accgcctcgg ccgcaaccgc tgcggccgag
    11461 gtcgagaaga caagctcgag cacaccggac cggcgcccag cagggcgcag cgacacgtcg
    11521 tcgtcgggtg agccgagcac cggatggatg agcgtgcgag cctctcgcct ggcggcgaac
    11581 ccgtgcacga tggtcggcac gatcgtgccc gtgctgtgcg tgatcgtcgt catcagtccc
    11641 acccatcgtc gacagtgatc cgtccgcgca gcctgaagct cttcccgtcg ttcgccgcga
    11701 tgaacccgtt gacctggttc tgcgcatcac gcagggccgt cttcaccacg aggctgatcg
    11761 cccggcgatt caattgcgtg attgcctcgt ccgtgtcctt cttgaaccga gacacatcgg
    11821 atgtcgcttg cccggtcttc gcatcgatca cgatctcctg accgtccggc agggtgatca
    11881 gcgtgttgcc gaagtcgtct accgcctgag tcgccgactc cgcgcccgcg atcgtggcga
    11941 gcagctgctc ggagaccgct cgggccgcga tccgcgcctg atccgcggca tccgcgtttg
    12001 ctgacttcac gccgtcgagg tcgccgagca gagcggagac ggcgttgtgc tgatcccggt
    12061 aggcgtcggc ctgctcggtt gtgacggtct tgctgccttc gccgacagcc ttgagcgtgt
    12121 cccagtactc gcgctcggac tcggtgagcc gaccgtgcac ctgctcgagt gaggcggcgt
    12181 cgccagcgag cgcacggtag atcgtctgca agtccagacc ggtgcgcttg ctgacttcca
    12241 gtgcgttctt ccgctgctcg gcgaagtctt caccgagagc gatctcgttg atctgggaga
    12301 ccagggtcgc ggtgtcgatg tagtcgcgtc ctccgtcgac ggcggccttc cacgcctcgg
    12361 agaagtactg tcggagcttc tgcgtgcggt cgttctgttt ctcgagttcg gcggtgacga
    12421 ggccgagacc ggtcgcgccc gcggcgaggg cagccgcacc aagaatgccc gcggcaccac
    12481 cggtccctgc gagggtggcg gcgagcccgc cgagggtgtc ctgtccgact tggccgagat
    12541 cggccagatt gccgcggacg gatgagacgg cctcgccgag gttctgtccg atttcctgcg
    12601 ttacctcgcc ggcgccttca cgcagccgcg acatgccctt ggcgccgtcg tcgagtgcgt
    12661 cgtcggatgc gttcttcatc ttgcggtagg agtcgcggta ctcccgctcg atcgcgttgg
    12721 cggtgcgctt ggtctcgtcg ccgagcttct ccgtggcctt ctgatcctcg cggagctgct
    12781 tctcgagctg ctccgggcca cggttgcggc cgagctcgag cagctctttc tgggcgtcct
    12841 cgagcggggc gataaccccg gtgtcgatgc cctgcttgaa tgccttggtc tcggacccga
    12901 tgccgacctg atacccgcca gccatggcta cttgctcacc ttctcgaacg tctcagcgac
    12961 cgtgcggtac gcggtctgcc acagcagcgc gccggcccgc gggatgaacg cctgcaccgc
    13021 cgggaagaac acgtacccgc cgcgctgggg ccggcggaac acgccaccca tccgccgctt
    13081 gtactccttg cccttccggg accgggacac gaccgtgcgg ttcgggtcgt tgccgaactc
    13141 ggcaccgccg gcgaggacgg atgcgggcac tccgtcgccg atcttcccga tcgtcgccga
    13201 ccgcaggatc gcgttgtcgt cccggatcga cactcgggcg gtgtcgacga tgacacgctg
    13261 ttgcaggtgc gtctcagccc gctcggtcat ctcgctcttg aacgccggtt ccagcacgcg
    13321 ccgcgccgca gcacggtact gcttggagac ctcggcgggg agcgagcgca cggcttgcac
    13381 cagcacggcg aagtcgcgcg agacgagcag gctgagacgc atggctcagg ccaggacggg
    13441 ctggccggtc accggcagcg cgaccgagga ggtcgcgaac gcgccgacgc cgccaccgat
    13501 cccgcccggc agcagggtca ccgtggcggt gatcttcttc gcgcccgtgc ccttcttggg
    13561 cgtgaacgtc gcggtgacct gcttgccggg attggcgagc aggtactggg cgaagctgtt
    13621 cgcggtctcg tagtcctgcg cgtagtcgat gttcgcgacc cactcggcgg cgccgacgtc
    13681 ctggaacacc gcggtcgggg tcaggccctt ccacgtctgc gtcggcacgg tcggggtgat
    13741 ctcgacccgc gagacgtgcg cctcgtagtc gccgacatcc gtggcgccgt ccttgatgtt
    13801 gagcgtgcag tcacgcagga tgaacggctt gacagcgata gcaaccatga tcttctactc
    13861 cttgttgatg acggccgtga cggtgaagtc ccagccgagc cggtcggtct tcgcatcccg
    13921 gaccttgcgg gcggtttccc acgagatcgt cgagtgcggg tccagggcgg tgaccagttc
    13981 gatcacctgg ttgtcgaggg tgttctccgc cttcacgtcg tcctcgtgtg gtgagagcag
    14041 cgtgaggacg atccgagcgc gcagcgcgcc gagcggggcc tcggggagcg ggtccagctc
    14101 gagcagcttg aacacgagcg tcgggacggt gatcgccgcc ggggtgtcct ggttcgggat
    14161 gtaccgccac gccggcgacc catccggccg cttcggcagc aacggcttga gctgcgcgga
    14221 gaaccacgcc cgcaggtcag aggacatgag gcttcccatc caccgggcgg atgatctgct
    14281 tgatcgtgcg gtcgagcggc cgaggctgga acacgaactc accttcaccg aggttccccg
    14341 cctgcgagac cgtgcccgcc gtcagcaggt tcttcgcctg ctgcaactgc gcatacacgt
    14401 accgggtcgg cacgccgtcg ccgtccgccg ggccggcgac ccacttgaac gtccccgggc
    14461 cggtgatcgg ctcgaggccc gtcgcggtga cgttgatccc gagcgccccg aactccgcga
    14521 cctcgacgat cgacacttga acagcgcggt cgacgatgga ctggtagacg gtcccgatcg
    14581 ggccgaacag gtccggcagt gggagtctgg cttcctcgtc ggggtcggcg atggtgacgt
    14641 cggcggtcac gatgtcgccc tcgcgggtga gggtcgcggt cgacccgagc ggccatgtga
    14701 gcgtggccgg ctcgtagacg gccggggtgt cggcgggagc gtaggcgatc acctgttcgc
    14761 gggcgacctc gaggatcatt ccgcacacct cgaggttctc gatcggtgcg tcactccacg
    14821 cggcgagcag ccgcgtctcg tcggtcgcag tgaaccaggt gctcatcgtc tacgctcccg
    14881 ccgtcgctca ggcgtccgcg acgccgatga gggcgagcgc ctcgggccgc ttcacgaacg
    14941 tctgaaggta gccgtgcacg gccttgtcga tgccaccgcg ggcgaggtcg agggcgtcga
    15001 cctcgagcag gccaccggcg ggacggtcga agtcgatcgc gtacgacgcg ccgacgagcg
    15061 cggcgccggt gtcctcgatg ccgatgtcgc cgttcacgat ctgcacgttg ccgtcgactg
    15121 tgcccgtgcc cgcggtggac agggcgatgt tgacgaacgc cgggaggttc tgctctccgc
    15181 cggcggcgta gatcagttcc tcgaagtcga tgtcgttcac gatcgcgaac gtcggggtgt
    15241 cgcggcggcc gtccttcttg cgcttcttga ccgcgaggat gccctggatc aggtgcttca
    15301 gcgcggtcgg gtagttgtcc ggcagcgcgg ggctgttcgg cgcgatgggc gcgccggcgg
    15361 cggcgacggc gtaggacagc gcgaggtcgt cctgccagat gaggtagtcc tcctgctgca
    15421 tgcgcaggaa cgcgagcacg aactcgagcc cgcccgagag atcccagaac tcccggccga
    15481 tgtcctcgcc catcgcccag ttgtcgcgga cgctgctgtg ctcggtcacg aatcccttgt
    15541 tggacttgat cgcggtcttg ttgccggccc acgtgccgtc catcggcgcg tcgagcggcg
    15601 cggccgcggt accgcggtgc accttgtagc ccttcttgcc accgatgctg atctcggttc
    15661 cggggttgaa gagcgtgatg tactcctgca cgtaggtgat cccggcggcg atgtcgccca
    15721 tccaggtcgg ctggatggcg gcgccggcat cgctgatcac gatgtcctgc agtgcagcga
    15781 gcacctcgac ggcctgccgg tcggcgcggt tgttcttcac ggcggcgatc gcgctcgcga
    15841 tctcctgacc ggacgggccg cgggatgcaa gcaccgcggt gctgccaccc ccgttgggtg
    15901 cgatggtgcc ggggacggcc ccggcctgga cggtttcctg ctcggccacg gtgggctcct
    15961 tttcggtgtc ggccgaggtg atctcggtct cggtggatgt ggtgacggtc gtggtctggg
    16021 tgccgccctc gacgtcctca gtcgtcgagg aggactcgta gacgcgcttg tacttgcggc
    16081 cgtcctcgtc ggtgaagatc gtctcggccc ggtcctggta gtcagcagcg ctgtcgtcgg
    16141 cgagttcggc gagcacgcgg gcggatggga acgcgccggc cacgacctgc gccgccgcgg
    16201 cgagcacgcc gttgcgggcg atgcgctgcg cgtcgatgtc ggtcttgaac tcggccgaca
    16261 gagaccggcg ggaggtgtcg gcgagggcgg cgtcaccggc cggggtgcgt ccgatgctga
    16321 acgtcgccat gatcccgcgg gctgtctccc agaggcgggt cgcgcggccg accggctggt
    16381 agcggtcgtg gtcaatgttg agggtgacca cggacgggtc ggcgggcagc tcgatcgacc
    16441 cggcctggac ggtgaagcgg ccggcgttcg tgcgcccctc ctcgttgaac gggatcaaca
    16501 gtcccgtcat ggagcggtcg tcgaggtcgg cgagaatctc gccgccctgg atggtgatgt
    16561 catgcatggt cagtcctcgc ttgtctgctg ctcgggtggg gtcggcacgg ccaggaaccg
    16621 ggacgtgtcg agtcggatca ggtgccccgg cgcgcacacg tcgtccagcg acatgcgggc
    16681 gccgatcgct ccggcatact tgcggacgcc gaagtcccac acctcggagc gctgcgcccc
    16741 gttctgcacg cccgtgtacc gcatctcggt gccaccgccg gacccgccct gccggacgcc
    16801 ctcgagcatc gacgcgggca ggccggcatg gttggcgagg tcgaggcggt tcgcgttgcg
    16861 gccggtctcg aacaggtcgg tggggatcgc gcccgacacg tcgacatcga cgtaggtcgg
    16921 cttcatcgcg acggcaccgc cagcagccga ccgcgccgcg atccacttct gccggaactc
    16981 ctcgcgctcg tcccagcccc acccgtccca gatgtccttg tcgacggtga ggatggtctg
    17041 cgcgatgggg ttgtcgatgc ggtcgcggta ggcgtcggcg atggtgcggg catccttgat
    17101 gtcgttcagc gcgtcgacga gcatcccgtt cttgccgtac ccgagccgga tcgggacgac
    17161 gcgctgccgg tagcgggcgt cgatccgtgc gttcacggtg atcgtgccgt cgtccccgat
    17221 gttccacagc gcccgcggga tgtgcagcgc gtcgaccggc agcctgtccg tgccgagctc
    17281 gaaaccgatc gccgcccacc cggacatgaa caggtcggac gcgaccgccc agcgcaggtc
    17341 gtacggcggg atgccggtct tcgtgttcgt cagccactcg ggctgcttct cgaggcgggt
    17401 cggtaccagc tcccccgtgt ccggatcctc gaccatgcgg gtgccgtcgt acgcgaccca
    17461 cggcatgtcg gcgatgacgg aacagatcac gtcgtgcgcc cggtcgaccg ccggcacccg
    17521 gaacgcggtg tcgcgggtga tcgcgtcagg ggcgaccggc agcgcgtcgg cgacgaacat
    17581 cgagtggatg ctgtcctgcg gggagtacca ggacagcaga ccaccggtca ccgccgacgc
    17641 ggccgggccg aggaacagat cacggaagaa tcccacgcct cagagcgtgg acccgacctt
    17701 acacattcat gattcgggtc ggcgtgtcgg catcttccgg atgccgtcgc gcgtacatct
    17761 tcgccgcgtt ccgcgccctg tattccccac ggtgcgcgcg cgcctcgtga ccggatgccg
    17821 actggtgcgc gccgcccatc gtggccgcga aagcatgcca gtgcgggcat gcggtgcagc
    17881 tgatcgtgac gccgccggcg gacttctcga ggcggatgct gggtcgagtc atggtctgct
    17941 cctcagaagt ggaatgcgtc ggcggggttg acgtgggtgg gtgcgtcggc gaggaaccgc
    18001 agcgcgagcg cggcggcctc gagcggtgtc acgtcggcct cggggtcgtc cttcggcgcg
    18061 ccgaaccgcc acgtgccgta gttcccgaac gcctgccggg tcgcgatccg cagcgccttc
    18121 tcgagcgccg gcgtcgcgaa gatgatcagg tcgtcgtcct cgagggcctt catcagaccg
    18181 accgccgacc cggggatgtc ggccggcttg gtcggctcga gcctcggtcg cgggctcgcc
    18241 ttggccagct cgtgctcggc gatctgctcc gtgtacccgc gcttgtcgta ccacagcgtc
    18301 gtcggccggc ggcgcagcct gacgagcacc tcgcgcgaca ggccggtggt gccctgctgc
    18361 caccaccaca gcgcgaccgc ccgcttgcgg atcacctcgc ccgtggcggc agtcaccagg
    18421 tcgccgtctt cctcgtgctc ccacgcgacc gcgagggatg ccgcgtgacc gaggtggtgc
    18481 accttgagcg cggcagcgaa ccgtgccggc ggctcgggcg gaacgccctt gcgcagcgcg
    18541 cgctcgagca tggccggcgg gatcgcgaca tccgccgcgc cctcgaatcc gaactgcccg
    18601 ccgtactcga tcaggaagtc gtcaaggttg ctgaacttgt cgaacgaccg cttgggggcg
    18661 tcggtgagcg tggtgaatcc gaggcccggg tgcgtggcct cgatccactc ccgcatccgc
    18721 ccgcccgtcg cgcccgtctt cgggtgcggg atgtccggct cccacgagac gagctgcgcg
    18781 cggtcgatgt tctccgggat gccgtgccag agcaccgcgg cgtcctcgtc gtgcaggttc
    18841 tcccacagca gttggccggt gcgatacttg gcgccggtcc cggcgagcac gaactgcgag
    18901 cccggcttgg tgtccatcgt cgggatgacg gcgcggttca cctcgtcacc ctgctcgaca
    18961 tccgcctcac cggcctcgtc gccgaacgcg aagtcgaatc cgccggatcg gaacatgtcg
    19021 ccattcggcg tgccgacgcg cagcagcgcg cccgtgtcac ggaactcgag ctgctcccca
    19081 cccttcgata gaccgatcgt gatcgggctc ttgcgacgat ccggccacag cttgtcgagg
    19141 tgcttcacga tgtcctcgcg gaaccgctgc cccgccttca tcccggtcgt gaacatcgtc
    19201 caccccaccg tgtagtcctc ccgcgaccag cagcgcccca tcatcaccgc ctgcaccgcg
    19261 gtcgtcttcg tcgaccggcg cggctcgaag atcacgttca tgaaccgccc ggccgcgagc
    19321 agatcggcga ccatgagctg catcggcgac ggaccccacc cgagatcgcc ggcacgttgc
    19381 cgctcgtcga ggcgcagcag ccgcgccccc tggatgaact caccccgcga ctgctcggtg
    19441 gtcaccagcg cgtcgagccg gatcggatcg agatggttga tccgcgaccg ccactcgagc
    19501 cagttgccct cgtcccacag atcggccagc gtcggcgttg cgctcatgag aatctccagt
    19561 tcagggggaa aaagtgctta tggagccgaa ggcgggggtt gcaccgaccc tcaaaaaaga
    19621 ccgccgtcac cacggcgcga gcccgtcgcc gccgcgctga ccagccggcc gagccgggtg
    19681 agctgg
//
LOCUS       DATMSX010000393        11409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147655,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000393 DATMSX010000000
VERSION     DATMSX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147655"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..5047
                     /locus_tag="VN041_12200"
     CDS             29..5047
                     /locus_tag="VN041_12200"
                     /inference="COORDINATES: protein motif:HMM:NF039610.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HWU29833.1"
                     /translation="MRVTDDATTSPDFWNAFDFTGIGAITAPAGADQVVVSVYVPVDG
                     ELTWVDSAATPIGEAAVPVPAEQYGDVQGIRFAFSRADGAYLSPAVPAPNWSTTAAFT
                     VQLRDDYRDSGDPVPLEGTIDNTVTAVSDRRTGSVVEDTANAEIALSPGTHDLAVHKR
                     VNNGTTHFATAGDLVPWDLTFRNQGTGYLTITELRDLLPPELVYLGDEPIYIPGEGGL
                     LPAPEAFTQEGDELVFTWAEGSRMAPGESFSIRILLELQPGLLLGEQAVNEITVQTVE
                     ELGSCTNIDPAGAVTDAWDADPTTCGTTEYVTPTDGTSLFVVKGVRGERDGAINTTNP
                     AIVCEQSLNATGGAYYRSPCAANSQIGGTDDWVLRAQNGGTRPISDMILFDQLPVAGD
                     QALISGASRGSVYRPQILDAIELAAPAGTTTVVEVTTSTGVCVGTWSNLTEQEPCAQN
                     GEVWVPADDATDWSTVSGLRVALDFSTTAAGALQPGAFVDITYSSTNVVASEANPSGA
                     PSAVPARDSFAWNQFGVKYRELGATGYLKISPSPVGVHLVFGSIEVAKEITGDAAGYA
                     PDEFLVDVVCTIDGVQLDLGDAAVLELNSANEHTSRIDGIPLGAACDVTEQGAIGRFG
                     ETTRTGTPVTLVVAEQNEELAVPAAQIATIGNDYAYSGLAVTKTVDTDATEGEFGPFD
                     FTLSCTTATGTPVVFDGDATELAFTLEDGETFTAPENTIPARSTCVIAEVDSADADDI
                     VIVGDNVTDNGDGTAMVLVGTETANVEFANGYDSGILTVQKVVDGEGAALYGAGPFDF
                     TAVCTYEGQTLLNEAFQLDANAQRTFGVYPTGTSCIVEETGTGGATSTAIDPADGTVV
                     ITDEADAAATVTATNTFDLTSIQVVKEITGPAAAYADDEYLVDVVCTVRGDGVPLGDD
                     AVVELNEANGFAYRIDGIPTGATCDVTEQGELGEYGETGRTGTPTVIDVTEVVAPDAE
                     VPAAQTATIANDYTFAGLAITKRVDTEAQADFGPFDFTLSCTSATGIPVLLDGDATEV
                     EFTLEADETYTAPEDTIPANATCLIAEIDSAAADEIVIVGDDVTDNGDGTATVQVGTG
                     TANVEFTNGFDAGILTVAKVVDGAGAEMWGAGPFEFTAVCTYRDQTLLDETFVLEANA
                     QRTFGTYPTGTECVVTETETAGATTSVLDPTDGVVTLSEGESATVTATNTFALTAIEV
                     TKTVTGDAADYASDEFRADVVCTVGGADVPLGADATVVLDEANDFTRRIDGIPTGATC
                     EVTEQGAVGEFGETTRPSPVAIVTADAVVTEDDEVPAEQTAELVNDYAFSGLSITKRV
                     DAEAEAGYGPFDFTLQCTSATGIPVTFGDAGTELTFTLEADGTFTAPADTIPARADCV
                     ITEVGATADGIVITGDGVTDNGDGTASVHVGVDAAAVEFTNSFDSGVLTVSKVVDGAG
                     ATLYGTGEFTFTAVCTYDGQTLLDETFALAGGATRSFGPYPTNTECVVEETDAGDATS
                     TVLDPSDGTVVLEADGTAAVTATNTFDVASITVTKERVGDLDAEGANGSFTVLLECAD
                     GVTVPGGAERVLSAQGGYTTVFRDLPVGADCRISETDAGGAVQTTIVVTAADGTAVTS
                     DGTSADVALDGDVDVEITNEFVAGLPATGLTLPIVLGILVLLLLGGGVLVLLIARRRR
                     ALEA"
     gene            5762..6070
                     /gene="rpsJ"
                     /locus_tag="VN041_12205"
     CDS             5762..6070
                     /gene="rpsJ"
                     /locus_tag="VN041_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWU29834.1"
                     /translation="MAGQKIRIRLKSYDHEVIDTSARKIVDTVTRAGATVVGPVPLPT
                     EKNVVCVIRSPHKYKDSREHFEMRTHKRLIDIVDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            6079..6738
                     /gene="rplC"
                     /locus_tag="VN041_12210"
     CDS             6079..6738
                     /gene="rplC"
                     /locus_tag="VN041_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWU29835.1"
                     /translation="MADINAKISKGLLGTKLGMTQVWDENGKIVPVTVIELAPNVVTQ
                     VRTPEKDGYNAVQIAAGQIDPRKVNKPLTAHFEAAGVTPRRHVTEIRTADAADYALGQ
                     ELTVDGLFEAGQLVDVVGTSKGKGTAGVMKRHNFKGVSASHGAHRNHRKPGSIGASST
                     PSRVFKGMRMAGRMGGERVTVLNLTVHAIDAEKGLMLVKGAVPGARGRVVYVRNAVKG
                     A"
     gene            6741..7403
                     /gene="rplD"
                     /locus_tag="VN041_12215"
     CDS             6741..7403
                     /gene="rplD"
                     /locus_tag="VN041_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWU29836.1"
                     /translation="MADSTLALDVLKADGKKAGSIELPAELFDVKTNIPLIHQVVVAQ
                     LAAARQGTHSTKRRGEVSGAGRKPFKQKGTGNARQGSIRAPHMTGGGIVHGPKPRNYA
                     QRTPKKMIAAALLGALSDRFRGERLHAVETFGIDSAPSTKTAASLLAQVAGPKNVLVV
                     TQRDDEVTLKSVRNLPNVHWLTYDQLNAYDVLVSDDIVFTKAALEAFIASKTGATEEV
                     SA"
     gene            7400..7723
                     /gene="rplW"
                     /locus_tag="VN041_12220"
     CDS             7400..7723
                     /gene="rplW"
                     /locus_tag="VN041_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWU29837.1"
                     /translation="MTEQATVLTTALNKDPRDIILKPVVSEKSYSLIDEGKYTFLVDP
                     RASKTEIKLAIEKIFNVKVAAVNTINRVGKARRTRFGTGKRKDTKRAIVTLKSGTIDI
                     FTAIG"
     gene            7744..8583
                     /gene="rplB"
                     /locus_tag="VN041_12225"
     CDS             7744..8583
                     /gene="rplB"
                     /locus_tag="VN041_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWU29838.1"
                     /translation="MAIRKYKPTTPGRRGSSVADFAEITRSTPEKSLLRPLSKTGGRN
                     NQGRITTRHIGGGHKRQYRVIDFRRNDKDGIDAKVAHIEYDPNRTARIALLHYFDGEK
                     RYILAPNKLKQGDVVESGAGADIKPGNNLPLKNIPTGTVIHAIELRPGGGAKMARSAG
                     ASVRLVAKDGPYAQLRLPSGEIRNVDARCRATIGEVGNAEQSNINWGKAGRKRWKGVR
                     PTVRGVAMNPVDHPHGGGEGKTSGGRHPVSPWGQAEGRTRHANKESDKYIVRRRNAGK
                     KRK"
     gene            8596..8877
                     /gene="rpsS"
                     /locus_tag="VN041_12230"
     CDS             8596..8877
                     /gene="rpsS"
                     /locus_tag="VN041_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWU29839.1"
                     /translation="MPRSLKKGPFVDEHLLRKVVGQNEAGSKNVIKTWSRRSMIIPAM
                     LGHTIAVHDGRKHIPVFVTETMVGHKLGEFAPTRTFRGHVKDDKKGRRR"
     gene            8877..9275
                     /gene="rplV"
                     /locus_tag="VN041_12235"
     CDS             8877..9275
                     /gene="rplV"
                     /locus_tag="VN041_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWU29840.1"
                     /translation="MVDSIARVKHIRVTPQKARRVVALIKGKQAQEALAILKFAPQGA
                     SEPIYKLVHAAIANAQVKADRDGEYLDEQDLYVKNAYVDEGTTLKRFQPRAQGRAFQI
                     KKRTSHITVVLATPEVADAAPAAKTKKASK"
     gene            9275..10021
                     /gene="rpsC"
                     /locus_tag="VN041_12240"
     CDS             9275..10021
                     /gene="rpsC"
                     /locus_tag="VN041_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWU29841.1"
                     /translation="MGQKVNPYGFRLGITTDHVSRWFSDSTKPGQRYADYVAEDIKIR
                     RLLQTQLDRAGVSGIEIERTRDRVRVDIHTARPGIVIGRRGAEAERIRGDLEKLTGKQ
                     IQLNILEVKNPEADAQLVAQGIAEQLSARVAFRRAMRKGLQGAQRAGAKGIRIQVSGR
                     LGGAEMSRSEFYREGRVPLHTLRANIDYGFYEAKTTFGRIGVKVWIYKGDLTNKELAR
                     EQANMKPQRERGDRRRAPRNEAPVAEGASA"
     gene            10021..10440
                     /gene="rplP"
                     /locus_tag="VN041_12245"
     CDS             10021..10440
                     /gene="rplP"
                     /locus_tag="VN041_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWU29842.1"
                     /translation="MLIPRKVKYRKQHHPKRTGAATGGTKVSFGEFGIQALTPAYVTN
                     RQIESARIAMTRHIKRGGKVWINIYPDRPLTKKPAETRMGSGKGSPEWWVANVKPGRV
                     LFEVAGVNEELAREALTRAIHKLPLKARIIKREEGDA"
     gene            10440..10754
                     /gene="rpmC"
                     /locus_tag="VN041_12250"
     CDS             10440..10754
                     /gene="rpmC"
                     /locus_tag="VN041_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWU29843.1"
                     /translation="MAIGTKELAPAELDTFEDQRLVEELRKAKEELFNLRFQSATGQL
                     ESHGRIRAVKRDIARLYTVIRERELGIRATPAPVEAPAKKASKAKAKKAEEADAPKEE
                     AE"
     gene            10754..11068
                     /gene="rpsQ"
                     /locus_tag="VN041_12255"
     CDS             10754..11068
                     /gene="rpsQ"
                     /locus_tag="VN041_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWU29844.1"
                     /translation="MAEKKAAEAVEHAAHDVRDADARGYRKARRGYVVSDKMDKTIVV
                     EVEDRVKHPLYGKVIRQTSKVKAHDETNSAGIGDLVLINETRPLSATKRWRLVEILEK
                     AK"
     gene            11065..>11409
                     /gene="rplN"
                     /locus_tag="VN041_12260"
     CDS             11065..>11409
                     /gene="rplN"
                     /locus_tag="VN041_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWU29845.1"
                     /translation="MIQTESRLKVADNTGAKELLTIRVLGGSSRRYAGLGDTIVATVK
                     DAIPGGNVKKGDVVKAVIVRTVKSTRRPDGSYIKFDENAAVILKNDGEPRGTRIFGPV
                     GRELRDKKFMKIV"
BASE COUNT         2101 a         4037 c         3700 g         1571 t
ORIGIN      
        1 cccggtcagc accctggcgc cggccgaggt gcgcgtcacg gacgatgcga ccacgtcgcc
       61 ggacttctgg aacgcgttcg acttcaccgg catcggcgcg atcaccgccc cggccggcgc
      121 cgaccaggtc gtcgtctccg tatacgtgcc ggtcgacggg gagctgacct gggtcgattc
      181 cgccgccacg ccgatcggcg aggcagccgt gccggtgccc gccgagcagt acggcgacgt
      241 ccagggcatc cggttcgcgt tctcgcgcgc ggacggcgcc tacctgtcgc ccgccgtgcc
      301 ggcgccgaac tggagcacca ccgcggcgtt caccgtgcag ctgcgcgacg actaccgcga
      361 ctcgggcgat cccgtccccc tcgagggcac gatcgacaac accgtgacgg ccgtcagcga
      421 ccgccgcacc ggctcggtcg tcgaggacac cgcgaacgcc gagatcgccc tgtcgccagg
      481 cacccatgac ctcgcggtgc acaagcgcgt gaacaacggc acgacccact tcgccaccgc
      541 gggcgacctc gtgccgtggg atctcacgtt caggaaccag ggcaccgggt acctgacgat
      601 caccgagctg cgcgatctgc tgccgcccga actcgtgtac ctgggcgacg agccgatcta
      661 catcccgggc gaaggcggtc tgctgccggc tcccgaggcg ttcacgcagg agggcgacga
      721 gctcgtcttc acgtgggcgg agggctcgcg catggcaccg ggggagtcgt tctccatccg
      781 catcctgctc gaactgcagc cgggcctcct gctcggcgag caggcggtca acgagatcac
      841 cgtgcagacc gtcgaagagc tcggctcctg cacgaacatc gatcctgcag gcgccgtgac
      901 cgacgcgtgg gacgccgacc cgaccacgtg cggcaccacc gagtacgtca ccccgaccga
      961 cggcacgagc ctgttcgtcg tcaagggcgt ccggggcgag cgcgacggcg ccatcaacac
     1021 gaccaacccc gccatcgtct gcgagcagtc gctcaacgcg accggcggcg cctactaccg
     1081 ctcgccgtgc gcggcgaaca gccagatcgg cggcaccgac gactgggtgc tccgcgcgca
     1141 gaacggcggc acgcggccga tctccgacat gatcctcttc gaccagctcc ccgtcgccgg
     1201 cgaccaggcc ctcatctcgg gcgcctctcg cggttcggtc taccgtccgc agatcctcga
     1261 cgcgatcgag ctcgccgccc ccgccggcac caccacggtc gtcgaggtca ccacctcgac
     1321 cggcgtgtgc gtcggcacct ggtcgaacct caccgagcag gagccctgcg cgcagaacgg
     1381 cgaggtctgg gttcccgcgg acgacgcgac cgactggtcg accgtgtcgg gcctgcgggt
     1441 cgcgctcgac ttctccacga ccgcggccgg tgccctgcag cccggcgcgt tcgtcgacat
     1501 cacctacagc agcacgaacg tcgtggcctc cgaggcgaac ccgtccggcg ccccgtccgc
     1561 cgtgccggcg cgcgactcgt tcgcctggaa ccagttcggg gtcaagtacc gcgagctggg
     1621 cgcgacggga tacctgaaga tctcgccgtc gcccgtgggc gtgcacctcg tgttcgggtc
     1681 catcgaggtc gccaaggaga tcacgggaga cgccgccggc tacgcgccgg acgaattcct
     1741 cgtcgacgtg gtctgcacga tcgacggcgt ccagctcgac ctgggcgatg ccgcggtcct
     1801 cgagctgaac tccgcgaacg agcacacgag ccggatcgac ggcatcccgc tcggcgcagc
     1861 ctgcgacgtg acggaacagg gtgccatcgg tcggttcggc gagacgacgc gcaccggcac
     1921 cccggtcacg ctcgtcgtcg ccgagcagaa cgaagaactc gccgtccccg ccgcgcagat
     1981 cgcgacgatc ggcaacgact acgcctactc gggactcgcg gtcaccaaga ccgtcgacac
     2041 cgatgccacc gagggcgagt tcggcccgtt cgacttcact ctcagctgca cgaccgcgac
     2101 cggcaccccg gtcgtgttcg acggcgacgc gaccgaactg gcgttcaccc tcgaggacgg
     2161 cgagacgttc accgcacccg agaacacgat cccggcgcgc tccacctgcg tcatcgccga
     2221 ggtcgacagt gcggatgccg atgacatcgt catcgtcgga gacaacgtca ccgacaacgg
     2281 cgacggcacg gccatggtgc tcgtcggcac cgagaccgcg aacgtcgagt tcgcgaacgg
     2341 ctacgactcc ggcatcctca ccgtgcagaa ggtcgtcgac ggagagggcg cggccctcta
     2401 cggtgccgga cccttcgact tcaccgcggt ctgcacctac gagggccaga cgctgctgaa
     2461 cgaggcgttc cagctcgacg cgaacgcgca gcgcacgttc ggcgtctacc cgaccggcac
     2521 cagctgcatc gtcgaggaga ccggcacggg cggcgccacc agcacggcca tcgaccctgc
     2581 agacggcacg gtcgtcatca cggacgaagc ggatgccgcg gccaccgtca ccgcgacgaa
     2641 cacgttcgat ctcacctcga tccaggtcgt caaggagatc acgggccctg ctgccgccta
     2701 cgccgacgac gagtacctcg tcgacgtcgt ctgcaccgtc cgcggagacg gcgtcccgct
     2761 cggcgacgac gcggtggtcg aactgaacga ggccaacgga ttcgcgtacc gcatcgacgg
     2821 catcccgacc ggcgcgacct gcgacgtgac ggagcagggc gagctcggcg agtacggcga
     2881 gaccggacgc accgggaccc cgacggtcat cgacgtgacc gaggtcgtgg caccggatgc
     2941 cgaggtcccc gccgcgcaga ccgcgacgat cgcgaacgac tacacgttcg ccggactcgc
     3001 gatcaccaag cgcgtcgaca ccgaggccca ggcggacttc ggcccgttcg acttcacgct
     3061 gtcctgcacg agcgcgaccg gcatccccgt cctgttggac ggcgacgcga cggaggtcga
     3121 gttcaccctc gaggcggacg agacctacac cgcgccggag gacaccatcc cggcgaacgc
     3181 gacctgcctg atcgccgaga tcgacagcgc tgccgccgac gagatcgtca tcgtcggaga
     3241 cgacgtcacc gacaacggcg acggcaccgc gacggtgcag gtgggcaccg gcaccgcgaa
     3301 cgtcgagttc acgaacggct tcgacgccgg catcctcacc gtggccaagg tggtcgacgg
     3361 cgccggagcc gagatgtggg gcgcagggcc cttcgagttc accgccgtct gcacctaccg
     3421 cgaccagacg ctgctggatg agaccttcgt gctcgaggcg aacgcccagc gcacgttcgg
     3481 cacctacccg accggcaccg agtgcgtcgt caccgagacc gagacggccg gcgcgacgac
     3541 gtcggtgctc gatcccaccg acggcgtcgt gaccctgagc gagggggaga gcgccacggt
     3601 caccgcgacc aacacgttcg ccctcacggc gatcgaggtc accaagaccg tcaccggcga
     3661 cgcggcggac tacgcatccg acgagttccg cgctgatgtc gtctgcacgg tcggcggcgc
     3721 cgacgtcccg ctcggcgcgg acgcgaccgt cgtgctggac gaggcgaacg acttcacccg
     3781 gcgcatcgac ggcatcccga cgggggccac ctgcgaggtc acggagcagg gagcggtcgg
     3841 cgagttcggc gagacgaccc gcccgtcccc ggtcgcgatc gtcacggcgg atgccgtggt
     3901 gaccgaggac gacgaggtgc cggcagagca gacggccgag ctcgtgaacg actacgcctt
     3961 ctccggcctg tcgatcacca agcgggtgga tgccgaggcc gaggcgggct acggtccgtt
     4021 cgacttcacg ctgcagtgca cgagcgccac cggcatcccg gtgaccttcg gcgatgcggg
     4081 caccgagctg acgttcacac tcgaggccga tggcacgttc accgcaccgg cggacaccat
     4141 cccggcccgt gcggactgcg tcatcaccga ggtcggcgcc acggcggacg gtatcgtcat
     4201 caccggcgac ggcgtcacgg acaacggcga cggcacggca tccgtgcacg tcggcgtcga
     4261 tgcggcggcc gtcgagttca cgaactcgtt cgactccggc gtcctcacgg tgagcaaggt
     4321 cgtcgacgga gcgggagcga ccctctacgg caccggcgag ttcacgttca ccgcggtctg
     4381 cacctacgac ggacagacgc tgctcgacga gacgttcgcg ctcgccggcg gtgcgacccg
     4441 atcgttcggc ccgtacccca cgaacaccga gtgcgtcgtg gaggagacgg atgccggtga
     4501 cgcgacatcc accgtgctcg acccgagcga cggaaccgtc gtcctcgagg cggacggcac
     4561 cgccgcggtc acggcgacca acacgttcga cgtcgcgtcg atcacggtca ccaaggagcg
     4621 cgtcggagac ctggacgccg agggcgcgaa cggatcgttc acggtcctcc tggagtgcgc
     4681 cgacggggtc accgttccgg gcggcgccga gcgggtgctg agcgcgcagg gcggctacac
     4741 gacggtcttc cgggacctcc cggtgggcgc ggactgccgc atctccgaga cggatgccgg
     4801 gggagcggtg cagacgacca tcgtcgtcac cgcagccgac ggaaccgcgg tcacgagcga
     4861 cggcacctcg gccgatgtcg cgctcgacgg cgacgtcgac gtcgagatca cgaacgagtt
     4921 cgtcgcaggt ctcccggcga ccgggctcac gctgccgatc gtcctcggca tcctcgtgct
     4981 gctgctcctc ggcggcggtg tactggtcct gctgatcgcc cgacggcgca gggcgctcga
     5041 agcctgacga aacggtgaga aggccgggtg cgcgatgctg gcatccggcc ttcggcatcc
     5101 tctatgctga gtgcagccgc ctggggatct tgcggctggt cggcgaaggg gttcgccggc
     5161 cgcaaccgca cggccgagcc gtgcacatga ctggggaacg actttgggga tcgatgcgtc
     5221 cgcgacgcct tctgcgcgtc gcgcgctgcc gcaacggcag tcgatccgcc gagccttcga
     5281 cggggatcgg ggcgctcctc ccgcgcccgg atccccgcgt tcggcgcgcc ccctcgcgct
     5341 gagcgcatcc ccacgtgggg cgaggcatgg ggatgcctcg cctcacggtc gtggcctcgg
     5401 atgccggcgc gacacgcccg ggtttgcaga accgaaatca gtcatggcag aataggtgag
     5461 ttccacattc cgggggtgcg ctatgcgctc cggccggatc actaccaggg cagtgcatag
     5521 acggcgcctc ttcgtgaggc agcagggttc taggccgcgg gtagaagaac tccccgacac
     5581 ctctgctgag aggttctgcc gtgcgccgcg aggggcacgg actgacagaa gaacagtggt
     5641 gcgcagatcg gctctccgga ggcgatcgag tgccgaggcg tgaacagcgc catggtgcgt
     5701 ccaatgcccc tgagtcaccc ggcccaggac ggtaagacgc ttaaagagag tgagcagaca
     5761 aatggcggga cagaagatcc gcattcgcct gaagtcgtat gaccacgagg tcatcgacac
     5821 gtcggcgcgc aagatcgtcg acactgtgac ccgtgcgggc gcaaccgtcg tcggccccgt
     5881 gccgcttccg accgagaaga acgtcgtgtg cgtcatccgg tcgccccaca agtacaagga
     5941 cagccgcgag cacttcgaga tgcgcaccca caagcgtctg atcgacatcg tcgacccgac
     6001 gcccaaggcc gtcgactcgc tgatgcgtct cgacctgccg gctgacgtca acatcgagat
     6061 caagctctga ggatcgacat ggctgacatc aacgcaaaga tttccaaggg tctgctgggc
     6121 accaagctcg gcatgacgca ggtgtgggat gagaacggca agatcgttcc cgtcaccgtc
     6181 atcgagctcg cacccaacgt ggtcacgcag gtccgcaccc ccgagaagga cggctacaac
     6241 gccgttcaga tcgccgcggg ccagatcgac ccccgcaagg tcaacaagcc cctcaccgct
     6301 cacttcgagg ccgccggcgt cacgccgcgt cgccacgtca ccgagatccg caccgcggat
     6361 gccgctgact acgcgctcgg ccaggagctc acggtggacg gtctcttcga ggccggccag
     6421 ctggtcgacg tcgtcggcac cagcaagggc aagggcaccg cgggtgtcat gaagcgccac
     6481 aacttcaagg gcgtctcggc atcgcacggt gcacaccgca accaccgcaa gcccggctcc
     6541 atcggcgcat cgtcgacccc gagccgcgtc ttcaagggca tgcgcatggc cggccgtatg
     6601 ggtggcgagc gcgtgaccgt cctgaacctc acggtgcacg ccatcgacgc cgagaaggga
     6661 ctcatgctcg tcaagggcgc cgtccccggt gcgcgtggcc gcgtcgtcta cgtccgcaac
     6721 gcagtgaagg gtgcctgaac atggctgact cgactctcgc gctcgacgtc ctgaaggccg
     6781 acggcaagaa ggccggctcc atcgagctgc ccgccgagct cttcgacgtc aagacgaaca
     6841 tcccgctgat ccaccaggtc gtcgtcgcac agctcgccgc tgcgcgtcag ggcacccact
     6901 cgaccaagcg tcgcggtgag gtctccggtg caggccgcaa gcccttcaag cagaagggca
     6961 cgggtaacgc ccgtcagggc tccatccgcg cgccgcacat gaccggtggt ggcatcgtcc
     7021 acggtccgaa gccgcgcaac tacgcgcagc gcacccccaa gaagatgatc gcggccgccc
     7081 tgctgggcgc gctgagcgac cgcttccgcg gcgagcgtct gcacgcagtc gagaccttcg
     7141 gcatcgactc cgctccttcg accaagaccg ccgcgagcct gctcgctcag gtcgccggtc
     7201 cgaagaacgt cctggtcgtc acccagcgtg acgacgaggt gacgctgaag tccgttcgca
     7261 acctgccgaa cgtgcactgg ctcacctacg accagctcaa cgcctacgac gtgctcgtct
     7321 ccgacgacat cgtcttcacc aaggccgcac tcgaggcgtt catcgcttcg aagaccggcg
     7381 caaccgagga ggtctcggca tgaccgagca ggcaaccgtc ctgacgaccg ctctcaacaa
     7441 ggacccgcgc gacatcatcc tgaagccggt cgtgtccgag aagagctact cgctcatcga
     7501 cgagggcaag tacacgttcc tcgtcgaccc gcgcgcctcc aagacggaga tcaagctcgc
     7561 catcgagaag atcttcaacg tcaaggtcgc agcggtcaac acgatcaacc gcgtcggcaa
     7621 ggcccgccgc acccgcttcg gcaccggtaa gcgcaaggac accaagcgcg ccatcgtcac
     7681 cctgaagtcg ggcaccatcg acatcttcac ggcaatcggc tgatccgggg gataaggaca
     7741 atcatggcta ttcgcaagta caagcccacg accccgggtc gtcgcggttc gtcggtggcc
     7801 gacttcgccg agatcacccg atcgacgccc gagaagtcgc tgctgcgtcc cctgtcgaag
     7861 accggtggcc gcaacaacca gggccgcatc acgacccgtc acatcggtgg tggccacaag
     7921 cgccagtacc gcgtcatcga cttccgtcgc aatgacaagg acggcatcga cgccaaggtc
     7981 gctcacatcg agtacgaccc caaccgcacc gcgcgcatcg cgctgctgca ctacttcgac
     8041 ggcgagaagc gctacatcct cgcgccgaac aagctgaagc agggcgacgt cgtcgagtcc
     8101 ggtgccggcg ccgacatcaa gcccggcaac aacctgccgc tgaagaacat ccccaccggt
     8161 acggtgatcc acgcgatcga gctgcgtccc ggcggcggtg cgaagatggc acgttcggcc
     8221 ggcgcctcgg tgcgtctggt cgcgaaggac ggcccctacg ctcagctgcg tctgccctcg
     8281 ggcgagatcc gcaacgtcga tgcgcgctgc cgcgcgacca tcggcgaggt cggcaacgcc
     8341 gagcagtcga acatcaactg gggcaaggcc ggccgcaagc gctggaaggg cgtccgcccg
     8401 accgtccgag gtgtcgcgat gaacccggtc gaccacccgc acggtggtgg tgagggcaag
     8461 acgtccggtg gacgtcaccc cgtatccccg tggggccagg ctgagggtcg tacccgtcac
     8521 gccaacaagg aatccgacaa gtacatcgtg cgtcgccgta acgccggcaa gaagcgcaag
     8581 taggagtaga ggaagatgcc acgcagtctt aagaagggcc ccttcgtcga cgagcacctg
     8641 cttcgcaagg tggtcggtca gaacgaggcc ggttccaaga acgtcatcaa gacctggtcc
     8701 cgccggtcga tgatcatccc ggccatgctg ggtcacacga tcgccgtcca cgacggtcgc
     8761 aagcacatcc ccgtgttcgt gaccgagacc atggtcggcc acaagctggg cgagttcgcg
     8821 cccacccgca ccttccgcgg ccacgtgaag gacgacaaga aggggcggcg ccgctaatgg
     8881 tcgactccat cgcacgcgtg aagcacatcc gcgtgacccc gcagaaggct cgtcgtgtcg
     8941 tcgccctcat caagggcaag caggcgcagg aagcactggc catcctcaag ttcgccccgc
     9001 agggcgcaag cgagccgatc tacaagctcg tgcacgctgc gatcgcgaac gcacaggtca
     9061 aggccgatcg cgacggcgag tacctggacg agcaggacct gtacgtgaag aacgcgtacg
     9121 tcgacgaggg cacgacgctc aagcgtttcc agccccgcgc tcagggtcgc gctttccaga
     9181 tcaagaagcg cacgagccac atcacggtcg tgctggccac ccccgaggtc gctgacgcgg
     9241 ctccggcggc caagacgaag aaggcgagca agtaatgggc cagaaagtca acccgtacgg
     9301 cttccgcctc ggcatcacca cggaccacgt gtctcgctgg ttctcggact cgacgaagcc
     9361 cggccagcgt tacgccgact acgtcgccga ggacatcaag atccgtcgcc tgctgcagac
     9421 gcagctcgac cgcgccggcg tctcgggcat cgagatcgag cgcacccgcg accgcgtgcg
     9481 tgtggacatc cacaccgcac gtccgggcat cgtcatcggc cgccgcggcg ccgaggccga
     9541 gcgcatccgc ggtgacctcg agaagctcac cggcaagcag atccagctga acatcctcga
     9601 ggtcaagaac cccgaggccg acgctcagct cgtcgcgcag ggcatcgccg agcagctgtc
     9661 tgctcgcgtg gcgttccgtc gtgcgatgcg caagggtctg cagggcgcgc agcgcgccgg
     9721 cgccaagggc atccgcatcc aggtctccgg ccgcctcggc ggcgcggaga tgagccgctc
     9781 cgagttctac cgcgagggtc gtgtgccgct gcacacgctg cgcgcgaaca tcgactacgg
     9841 cttctacgag gccaagacca ccttcggccg catcggcgtg aaggtctgga tctacaaggg
     9901 cgacctcacc aacaaggaac tcgcacgcga gcaggccaac atgaagccgc agcgcgagcg
     9961 tggcgaccgt cgtcgcgcgc ctcgcaacga ggcgcctgtc gcagaaggag cgtcggcata
    10021 atgctcatcc ctcgcaaggt caagtaccgc aagcagcacc acccgaagcg tacgggcgcg
    10081 gcaaccggcg gcacgaaggt ctccttcggc gagttcggca tccaggctct gacgcccgct
    10141 tacgtgacga accgtcagat cgagtccgct cgtatcgcca tgacgcgtca catcaagcgc
    10201 ggtggaaagg tgtggatcaa catctacccc gaccgtcccc tcaccaagaa gcctgccgag
    10261 acccgcatgg gttccggtaa gggttccccc gagtggtggg tcgccaacgt caagccgggc
    10321 cgtgtcctct tcgaggtcgc gggcgtgaac gaggagctcg cacgcgaagc cctcacccgg
    10381 gccattcaca agctgccgct caaggcacgc atcatcaagc gcgaggaggg cgacgcgtaa
    10441 tggcgatcgg caccaaggag ctcgcacctg ccgagctcga cacgttcgaa gaccagcgcc
    10501 tcgtcgagga gctgcgcaag gccaaggagg agctgttcaa cctccgtttc cagtcggcca
    10561 ccggccagct ggagagccac ggccgcatcc gcgccgtcaa gcgcgacatc gcgcgcctgt
    10621 acaccgtgat ccgcgagcgc gagctgggca tccgtgcgac gcccgctccg gtcgaggctc
    10681 cggccaagaa ggcgtccaag gcgaaggcga agaaggctga agaggccgac gcgccgaagg
    10741 aggaggccga gtaatggctg agaagaaggc tgctgaggcc gtcgagcacg ccgcacacga
    10801 cgtgcgcgac gccgacgccc gtggttaccg caaggcccgc cgtggctacg tggtcagcga
    10861 caagatggac aagaccatcg tggtcgaggt cgaggaccgc gtgaagcacc cgctttacgg
    10921 caaggtcatc cgccagacct cgaaggtcaa ggcccacgac gagaccaaca gcgccggcat
    10981 cggcgacctg gtcctcatca acgagacccg cccgctgagc gccaccaagc gctggcgtct
    11041 ggtggagatc ctggagaagg caaagtgatt cagaccgagt cccgcctcaa ggtcgccgac
    11101 aacaccggcg cgaaggagct gctcaccatc cgtgttctcg gcggttccag ccgtcgttac
    11161 gcgggcctgg gcgacaccat cgtcgcgacg gtcaaggacg cgatccccgg tggaaacgtc
    11221 aagaagggcg atgtggtcaa ggccgtcatc gtccgcaccg tcaagtccac gcgccgtccc
    11281 gacggctcgt acatcaagtt cgacgagaac gccgccgtga tcctgaagaa cgacggggag
    11341 ccccgcggca cccgtatctt cggaccggtc ggtcgtgagc ttcgcgacaa gaagttcatg
    11401 aagatcgtc
//
LOCUS       DATMSX010000394         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_6514,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000394 DATMSX010000000
VERSION     DATMSX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6514"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..1218)
                     /locus_tag="VN041_12265"
     CDS             complement(307..1218)
                     /locus_tag="VN041_12265"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HWU29846.1"
                     /translation="MTDDFVSDIFDPAEWVLAPGAEDYTDITAHVSRDGGVARIAFDR
                     PEVRNAFRPHTVDELYRALDIARQDPRIGAVLLTGNGPSPKDGGWAFCSGGDQRIRGR
                     DGYKYSESDTAVQDPARAGRLHILEVQRLIRFMPKVVIAVVPGWAAGGGHSLHVVCDL
                     TIASAEHGRFKQTDADVGSFDAGYGSAYMARQTGQKIAREVFFLAEEYSAQRAYEMGA
                     VNRVVPHAELEREALAMARTILTKSPTAIRMLKFAFNAVDDGMVGQQVFAGEATRLAY
                     GTDEAVEGRDAFLQKRDPDWSPFPYRF"
     gene            1276..1977
                     /locus_tag="VN041_12270"
     CDS             1276..1977
                     /locus_tag="VN041_12270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWU29847.1"
                     /translation="MRTPVKTPSRVRLALLALSAAAAFALAGCASAPGAAPRSSGQPS
                     ASQTQASGACTYAPDGSAAKDVTPPPADPTATGDAPVTVKTSAGDLAITLDGKSAPCT
                     VNSFLSLAEQGFYDDTKCHRLTTQGIFVLQCGDPTGTGAGGPGYAFPDELKGTETYPA
                     GTLAMANAGPNTNGSQFFIVYADTPLPAKYTVFGHLDEASTKIVADIASAGTEDGGPD
                     GAPKKPVTITSIAQG"
     gene            complement(2047..3003)
                     /locus_tag="VN041_12275"
     CDS             complement(2047..3003)
                     /locus_tag="VN041_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HWU29848.1"
                     /translation="MTATLTDRYITATVRRLPQQLQAEVRDELQTSIADAIDARAEQG
                     EPLEQAERAVLTDLGDPAALAAGYADRPLHLIGPKYYLFWWRVLTLLLATVPACAFVG
                     VAIGKAVAGEGIGAMIGEGIATALSAIVHVSFWTTLVFVILERTGSESTSKWDVDQLP
                     QEPGRGAGRGDLIGSLVMLAIFAAALLWDRFRGVTVVDGEAISVIDHALWPWWMSVVL
                     VLMLAEGAIAIAVYARGSWSTGLAVINTAVALCTASLFLTLLGRGMLFAPEFVQSVFT
                     RNGVDSGALAVLAVLTGVAVAGIAAWDIIDGWMKARRTAIAG"
     gene            complement(3005..3340)
                     /locus_tag="VN041_12280"
     CDS             complement(3005..3340)
                     /locus_tag="VN041_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU29849.1"
                     /translation="MNDSEFDGHLQELRRGTVVLASLQLLRTPGYGYGLLEQLASAGF
                     PTDANTLYPLLRRLEKQGYLDSEWNTDEARPRKFYRTSASGIRLADTLTQEVAAIASA
                     TAALREEGN"
     gene            3425..>3809
                     /locus_tag="VN041_12285"
     CDS             3425..>3809
                     /locus_tag="VN041_12285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29850.1"
                     /translation="MAAALEIRRVDPFHTADVDSWWEAYASARRAEMGENALLWSLEE
                     SRTELQQDSATTDRRAYLAVLDGVAVGSGSLALGLKDNLHSGMINVTVPPQFRRRGIG
                     SALLAHIEAEAVAADRRVLRGETAWP"
BASE COUNT          593 a         1357 c         1299 g          560 t
ORIGIN      
        1 atccggatcg ccgtgggcga cttggtgagc acggtgcgtg ccatgcgcag cgcctcgagc
       61 accgcacgcg cgtcgccgcc gtcgatcgga tgcagcctca tcggcgcttt ctccgggcga
      121 tccgggtcgc agttcgtccc gtctcgccgg tggcgttcgc tcccaggtcg cagttcgtcc
      181 cgtaatgacc cgcggatacc gggaccaatc gcgacctgca tgagaggggc catcggaaag
      241 cgggacgaat cgcgacctgg acggcggtgc gcggcgacgg cgtcgcggtg tcagcggccg
      301 acatcgtcag aagcggtagg ggaacggcga ccagtccggg tcgcgcttct gcaggaacgc
      361 gtcgcggccc tcgacggcct catcggtgcc gtaggccagg cgggtggcct cgcccgcgaa
      421 cacctgctga ccgaccatgc cgtcgtccac ggcgttgaag gcgaacttca gcatccggat
      481 ggccgtgggc gacttggtca ggatcgtccg cgccatggcg agcgcctcac gctcgagctc
      541 tgcatgcggc acgacccggt tcacggcacc catctcgtag gcgcgctgcg cggagtactc
      601 ctcggcgagg aagaacacct cgcgggcgat cttctggccg gtctggcgcg ccatgtacgc
      661 ggagccgtag ccggcgtcga agctgccgac gtcggcatcc gtctgcttga accggccgtg
      721 ctcggcggag gcgatcgtca ggtcgcacac gacgtgcagc gagtggccac cgccggcggc
      781 ccagccgggg acgacggcga tgacgacctt gggcatgaag cggatcagcc tctgcacctc
      841 gaggatgtgc agccgcccgg cccgcgccgg atcctgcacg gcggtgtcgg actcgctgta
      901 cttgtagccg tcgcgaccgc ggatgcgctg gtcaccgccc gagcagaacg cccacccgcc
      961 gtccttcggg ctcggcccgt tcccggtgag cagcacggcg ccgatgcgcg ggtcctgccg
     1021 ggcgatgtcg agcgcacggt acagctcgtc gaccgtgtgc gggcggaagg cgttgcgcac
     1081 ctcaggccgg tcgaaggcga tgcgggcgac cccgccgtcg cgcgagacgt gtgcggtgat
     1141 gtcggtgtag tcctcggcgc ccggtgcgag cacccattcc gccgggtcga agatgtcgga
     1201 gacgaagtcg tcggtcacgg catccagcct attccggcag gaaggatgcc gaacctcgtc
     1261 gcgtacgatc ttcgggtgcg cactcccgtg aagaccccgt cccgtgtccg tctcgcactg
     1321 ctggcgctga gcgccgcggc ggcgttcgcc ctcgcggggt gcgcctcggc tcctggcgcc
     1381 gcaccgcggt cctccggtca gccgagtgcg tcccagaccc aggcatccgg cgcctgcacc
     1441 tatgccccgg acggctcggc ggccaaggac gtcacgcctc ccccggccga cccgacagcc
     1501 accggcgacg cgcccgtgac agtcaagacc agcgccggcg acctggcgat caccctggac
     1561 ggcaagtccg caccctgcac ggtgaacagc ttcctctcgc tggccgagca gggcttctac
     1621 gacgacacga agtgccaccg gctcaccacc cagggcatct tcgtgctgca gtgcggagac
     1681 ccgacgggca ccggagccgg cggccccggc tacgccttcc ccgacgagct gaagggcacc
     1741 gagacgtacc cggccggcac gctggccatg gcgaacgccg gcccgaacac caacggctcg
     1801 cagttcttca tcgtctacgc cgacacaccg ctgccggcca agtacacggt gttcggccac
     1861 ctcgacgagg cgagcaccaa gatcgtggcc gacatcgcct ccgcaggcac cgaggacggc
     1921 ggccccgacg gagcgccgaa gaagccggtc acgatcacgt ccatcgccca gggctgatgt
     1981 cggtccgaca gacgcaggtc gcgtcccgga gagcttccgg gacgcgacct ctgggaaggc
     2041 cgcgcgtcag ccggcgatcg ccgtgcgccg ggccttcatc cacccgtcga tgatgtccca
     2101 cgcggcgatg ccggcgacgg cgacaccggt gagcacggcc agcacggcca gggcgcccga
     2161 gtccacgcca ttgcgggtga acaccgactg cacgaactcc ggggcgaaca gcatcccgcg
     2221 ccccagcagc gtgaggaaca gactcgccgt gcacagggcg acggcggtgt tgatcaccgc
     2281 gagcccggtc gaccagctgc cgcgcgcgta gaccgcgatc gcgatcgccc cctccgcgag
     2341 catcagcacc agcaccacac tcatccacca cggccacagc gcatggtcga tcacgctgat
     2401 cgcctcgccg tcgacgacgg tgacgccgcg gaagcgatcc cacagaagcg ccgccgcgaa
     2461 gatcgcgagc atcacgagcg agccgatcag atcgcctcga cctgcacccc gccccggttc
     2521 ctgcggcagt tgatccacgt cccacttcga ggtcgattcg gagccggtgc gctcgaggat
     2581 gacgaacacg agcgtggtcc agaacgacac gtgcacgatc gccgagagag ccgttgcgat
     2641 gccctcaccg atcatcgctc cgatgccctc accggccacg gccttgccga tcgccactcc
     2701 gacgaaagcg caggcaggca cggttgcgag cagcagggtg agcacgcgcc accagaacag
     2761 gtagtacttc gggccgatca gatgcagcgg cctgtccgcg tagccagcgg cgagcgctgc
     2821 agggtcgccc aggtcggtca gcacggcgcg ctcggcctgc tccaggggct cgccctgttc
     2881 ggcgcgggcg tcgatggcgt cggcgatcga ggtctgcagc tcgtcccgca cctcggcctg
     2941 cagctgctga ggcagcctgc ggacggtcgc ggtgatgtag cggtcggtca gcgtggcggt
     3001 catgtcagtt cccctcttca cggagcgcgg cggtcgcgct cgcgatggcg gcgacctcct
     3061 gggtcagggt gtcggcgagg cggatgccgg atgccgaggt gcggtagaac ttgcggggcc
     3121 tggcctcgtc ggtgttccac tcgctgtcga ggtacccctg cttctcgagc ctgcgcagca
     3181 gcggatacag ggtgttggcg tcggtgggga acccggcgga tgccagctgc tcgagcagcc
     3241 cgtatccgta accgggggtc cgcagcagct gcaggctggc cagcacgacc gtgccgcgtc
     3301 gcagctcctg cagatgtccg tcgaactcac tgtcgttcat gtttggaact atattgtgcg
     3361 tcccacacta ttgtcaatca cccatcgtta tatccggcac gcaacggcct cgtaggctgg
     3421 gagcatggca gccgctctcg agatccgccg ggtcgatccg ttccacaccg ccgacgtcga
     3481 ctcctggtgg gaggcgtacg cgagtgcacg acgagcggag atgggcgaga acgctctgct
     3541 ctggtcgctc gaggagagcc gcaccgagtt gcagcaggac tccgccacga ccgaccgccg
     3601 cgcctatctg gccgtgctcg acggtgtcgc cgtcggctcg ggctcgctcg cgctggggct
     3661 caaggacaac cttcactccg ggatgatcaa cgtcaccgtg ccgccgcagt tccgtcgccg
     3721 cggcatcggc tcggcactgc tcgcgcatat cgaggccgag gccgtggccg ccgatcgccg
     3781 ggtgctccgc ggcgagacgg cgtggccgg
//
LOCUS       DATMSX010000395         8402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_138809,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000395 DATMSX010000000
VERSION     DATMSX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138809"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..718
                     /gene="ruvA"
                     /locus_tag="VN041_12290"
     CDS             110..718
                     /gene="ruvA"
                     /locus_tag="VN041_12290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWU29851.1"
                     /translation="MISSLRGTVLHTASDHVIIETGGVGFSVFVPADVAHTAMIGEQL
                     RLHTSLIVREDALTLYGFGDRDELEIFGLLLSVTGVGPKSALGVLSHLTVDQIAEAVT
                     AEDDAPFRRVSGIGPKTAKLIVVQLAGKVQARVASAPSASASTAVVDQVAAALVGLGW
                     SEKVAAEAAVESAEEATDAERESVPALLRRTLAAMGPGAKRG"
     gene            711..1742
                     /gene="ruvB"
                     /locus_tag="VN041_12295"
     CDS             711..1742
                     /gene="ruvB"
                     /locus_tag="VN041_12295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWU29852.1"
                     /translation="MAEDHRDAGEALDETELAIEGALRPASLSEFVGQPKVRGQLQLL
                     LEAARIQERPADHILLAGPPGLGKTTLAMIVAHESGRPLRLSSGPAIQHAGDLAALLS
                     SLTPGEVLFIDEIHRMARSAEEMLYLAMEDFRIDIMVGKGAGATSIPLDLAPFTLVGA
                     TTRSGLLPNPLRDRFGFTGHLEFYDESDLERVIARSANVLGVALPADSLAEIARRSRG
                     TPRIANRLLRRVRDYALVHGSGASASIGDVRSALELYDVDAIGLDRLDRAVLDALVRR
                     FRGGPVGLSTLAVAVGEEAETVESVVEPYLVRIGFLGRTPRGRIAMPEAYRHLGVAHP
                     EGAALFDDL"
     gene            1832..2251
                     /locus_tag="VN041_12300"
     CDS             1832..2251
                     /locus_tag="VN041_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWU29853.1"
                     /translation="MSLMLFGLLAVMLVFMFMNTRKRQKQMKEQQEEKAAKTVPGAKV
                     LLQGGLYGTIVSFDPVDLDKPAQVELAPGVVIEVHSQAIIRVVDPTEETADDEADTED
                     AAATETTPAEGARDGIESIQVETPEETKARLERNNDN"
     gene            2287..4044
                     /gene="secD"
                     /locus_tag="VN041_12305"
     CDS             2287..4044
                     /gene="secD"
                     /locus_tag="VN041_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWU29854.1"
                     /translation="MASTSPTRHAWRVLLGLLVVTAVLFGINALGVYVFKADNDKPAS
                     SWTPELALDLQGGTQIILQAATPDGAAPSAEQLTQAVSIIRQRVDASGVSEADITTEG
                     GRNIVVQIPGAASKADRARITASAKLEFRAVLYTDAPVNSFVGEDGKSTPYPTPAATL
                     EATPTAKPTNASDPAYITPKLQAQFQAFDCKAEQESNPPEDQPLIACEFDNQGNAQAK
                     YILGPMELDGTVINDATAGREQQSGQWTVNLSMNAKGTKVFGEISQRLNANRIANLSP
                     RDQFAFVLDGKVISAPVMQAAILNGKPSISGNFTQESAKTLADQLKYGALPLSFTVQS
                     SDTVSATLGSQQLQYGLIAGLIGLGLVAVYSLLSYRALGWVIMASIAVMGVLTYIIIA
                     ILAWRMGFRLSLAGVAGLIVSIGFTADSFIVYFERIRDELRDGRSITGSIEYGWERAK
                     RTIYIAKSVNILAAVVLFILADATVKGFAFTLGLTTLIDVFIFVIFTHPVMQILARTR
                     FFGGGHRLSGLNAESLGAVRGAQTEYRTAETASKGRAARNKGARGEAERRQTIAERKR
                     AEALTAMGSKDAGKDSDAS"
     gene            4041..5036
                     /gene="secF"
                     /locus_tag="VN041_12310"
     CDS             4041..5036
                     /gene="secF"
                     /locus_tag="VN041_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWU29855.1"
                     /translation="MISMNEFGNNLYSGKTSFPFVGKRRLWFVIAILLVLGSVLVPLV
                     RPVQFSIEFTGGSQFMVVGPASTNQGLASDAVRSVVPGATTKVAIVNKKDVRVQTDRM
                     TPNQTQDVKAALASAFDVKADEVSDSFIGPAWGEGVTRQSLWGLAIFLALTFLILGIY
                     FRTWKMSAASILGLLDVLVITVGIYALAGFEISPAAVIGFLTILAYSLYDTTVVFDKV
                     RENTQEDAVSLQRTFGESVNLAVNQTLVRSINTSVVAALPVGAILFIGAFWFGAETLS
                     AISLSIFVGILVATYSTLFVAAPLYAAFRQNEPDILAHDKKVLQARERAAATTSV"
     gene            5109..7361
                     /locus_tag="VN041_12315"
     CDS             5109..7361
                     /locus_tag="VN041_12315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWU29856.1"
                     /translation="MADSQVASQGSSLRRLVPRIFSRAPRVNDLDNLIRTVRANHPRG
                     DLPIIEHAYRVAREKHEGQFRQSGEPYITHPLAVAQILAELGLGPRAIAAALLHDTVE
                     DTGYALSELTEEFGDEVAMLVDGVTKLDKVKYGESAQAETVRKMIVAMSKDIRVLLIK
                     LADRLHNARTWGFVPPEKSARKAKETLEIYAPLANRLGIQTIKSELEDLSFAVLHPKI
                     YAEINSLIAQRTPQREKYLQQVIEAIDEDLRELRIRGKVFGRPKQLYSVYQKMVVRGR
                     EFDDIYDLIGIRVLVNSVRDCYAVLGAIHARWTPLPGRFKDYIATPKFNLYQSLHTTV
                     IGPSGRTVEIQIRTHEMHQQAEFGVAAHWMYKERMNGGKVETRKTDADMAWLAHISDW
                     QAETADPSEFLDSLRFEIGAKEVYVFTPKGRVIGLPAGATPVDFAYAVHTEIGHRTMG
                     AKVNGRLVPLESELRSGDVVEVFTSKNPDAGPSQDWLTFVKSTRARNKIKGWFTKERR
                     EEAVEQGKESIARAMRKQNLPLQRLMSQESFAEVAHQLRYEDVTALYAAVGEGHVSTQ
                     SVLEKVTALVASEPDTSTGAIELPGHAPAREARSGDSGILVRGAADILVKLARCCTPV
                     PGDPIVGFVTRGSGVSVHRKDCVNVKALGSDPERFVEVEWAPTTKSVFRVQIQVEALD
                     RSGLLSDVTRVLSEHHVNILSATVSTTDERLALSRFVFEMGDSVHLDRVLNAVRRIDA
                     VYDVYRVTTS"
     gene            complement(7348..7905)
                     /locus_tag="VN041_12320"
     CDS             complement(7348..7905)
                     /locus_tag="VN041_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29857.1"
                     /translation="MHPALFYRPGERLSLPELGAARLDGVVFEVGEGFMPADTVEGAD
                     ARATSLRDLIPSHLAACGPTAAWIHGAGDAPPQIHHVRRAGPTRGRVGFSPRVVYHER
                     RAAPEELLSIGGIEVTTPLATAEELLFAATKPGGDDESWLRALLLLSPELIDALQPML
                     AATVRRPGRRRALQLMSELATIRTS"
     gene            complement(7972..>8402)
                     /locus_tag="VN041_12325"
     CDS             complement(7972..>8402)
                     /locus_tag="VN041_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HWU29858.1"
                     /translation="AEQAAAEEADSAPRIEAREALLEEAKAVADEPNLKRARQLLTRI
                     QRQWDEVGRIFPREKERALDDRMRSIEQALKSREDVDWKKNNPETKARANDMSSQLLE
                     AIEKLEQELAAAEKAGDKKAVKEATEALAARRAWLSALGA"
BASE COUNT         1336 a         2957 c         2806 g         1303 t
ORIGIN      
        1 cggctcgctg acgccggcgc agcgcgcgtg ggcggacgcc gagcgcggcg cccggggcac
       61 gactcgtgtc gctcgaacga atgtacgatc ggcccgatag gctggctgca tgatctcctc
      121 gctgcgcggc acggtgctgc acacggcctc cgaccacgtc atcatcgaga ccggcggggt
      181 cggcttctcg gtattcgtgc ccgccgacgt cgcgcacacg gccatgatcg gcgagcagct
      241 ccggctgcac accagcctga tcgtgcgcga ggatgctctg accctgtacg gcttcggcga
      301 ccgcgacgaa ctggagatct tcggcctgct gctgagcgtg accggtgtgg gcccgaagtc
      361 cgcgctcggc gtgctctcgc acctcacggt cgatcagatc gccgaggcgg tcaccgcgga
      421 ggacgacgcc ccgttccgac gcgtctcggg catcggcccg aagacggcga agctcatcgt
      481 ggtgcaattg gcgggcaagg tgcaggcgcg cgtcgccagc gcgccgtcgg cgtccgcgag
      541 cacggcggtc gtcgaccagg tggctgcggc actcgtcggg ctcggctggt cggagaaggt
      601 cgcagccgag gcggcggtcg agtccgccga ggaggcgacg gatgccgagc gggagtccgt
      661 tcccgcgctg ctgcggcgca cgctcgccgc catggggccg ggagcgaagc gtggctgagg
      721 atcaccgcga cgccggcgag gcgctggacg agaccgagct cgccatcgag ggcgctctgc
      781 gccccgcgag tctgagtgag ttcgtcggcc agccgaaggt acgcggtcag ctgcagctcc
      841 tgttggaggc cgcccgcatc caggagcgtc ctgccgacca catcctgctc gcggggcctc
      901 cgggcctggg caagacgacc ctcgcgatga tcgtggcaca cgagagcggt cgccctctgc
      961 ggctcagcag cggccccgcg atccagcacg ccggtgacct ggccgcgctg ctgtcgagcc
     1021 tcacgccggg cgaagtgctg ttcatcgacg agatccaccg catggcgcgc tccgccgagg
     1081 agatgctgta tctggcgatg gaggacttcc gcatcgacat catggtcggc aagggtgccg
     1141 gagcgacgag catcccgctg gatctcgcgc ccttcaccct ggtcggcgcg accacgagat
     1201 cgggcctgct gcccaatccg ctgcgcgaca ggttcggttt caccggccac ctggagttct
     1261 acgacgaaag cgacctcgag cgcgtcatcg cccgctccgc gaacgtcctc ggcgtggcgc
     1321 tgcccgcgga ttcgctcgcc gagatcgcgc ggcgctcccg tggcacgccc cgcatcgcca
     1381 accgcctgct gcgccgggtg cgcgactacg cgctggtgca cggctccggg gcatccgcgt
     1441 cgatcggaga cgtgcgctca gcgctcgagc tgtacgacgt ggacgcgatc ggcctcgatc
     1501 ggctggaccg tgctgtgctg gatgccctgg ttcggcgctt ccgcggtggc ccggtgggcc
     1561 tgagcactct cgccgtcgcg gtcggggagg aggccgagac ggtcgagagc gtcgtcgagc
     1621 cgtacctcgt gcgcatcgga ttcctgggcc gcacgccgcg cgggcgcatc gcgatgcccg
     1681 aggcgtaccg ccatctcgga gtcgcgcacc ccgagggcgc agcattgttc gatgacctat
     1741 aatcgctgaa ggcttcccgg attcccggtt tccccctcac gcaacgccgc gtccggcgtg
     1801 catctgaaag gcgcttgctc ctatggatcc catgtccctc atgctcttcg gtcttctcgc
     1861 cgtgatgctc gtgttcatgt tcatgaacac gcgcaagcgc cagaagcaga tgaaggagca
     1921 gcaggaggag aaggccgcca agaccgttcc gggcgccaag gtgctgctgc agggcggcct
     1981 gtacggcacc atcgtgtcct tcgacccggt cgacctcgac aagcccgcgc aggtggagct
     2041 cgctccgggc gtggtcatcg aggtgcacag ccaggccatc atccgcgtcg tcgaccccac
     2101 cgaggagacc gctgacgacg aggccgacac cgaggacgcc gccgccactg agaccacgcc
     2161 tgcggagggc gcgagggacg ggatcgagtc gatccaggtc gagacgcccg aggagacgaa
     2221 ggctcgtctc gagcgcaaca acgacaactg acgcgcagcg tcctctctcg cggaaagcgg
     2281 aaccccgtgg catccacatc cccgacccgc cacgcctggc gggtcctcct cggcctgctc
     2341 gtcgtgacgg cggtgctttt cggcatcaac gcgctcggcg tgtacgtctt caaggctgac
     2401 aacgacaagc ccgccagctc ctggacaccc gagctcgcgc tcgacctgca gggcggcacg
     2461 cagatcatcc tgcaggccgc gacgcccgac ggggccgcgc cctcggccga acagctgacc
     2521 caggccgtct cgatcatccg tcagcgtgtg gacgcatcgg gcgtgtccga ggccgacatc
     2581 accaccgagg gcggccgcaa catcgtcgtc cagatcccgg gtgctgcgtc caaggccgac
     2641 cgcgcgcgca tcaccgccag cgcgaagctc gagttccggg ccgtgctgta cacggatgca
     2701 ccggtgaaca gcttcgtcgg cgaggacggc aagagcacgc cgtacccgac gccggcggcc
     2761 accctggagg ccacgccgac ggcgaagcct accaacgcct cggatccggc gtacatcacg
     2821 cccaagctgc aggcccagtt ccaggccttc gactgcaagg ccgagcagga gagcaacccg
     2881 ccggaggacc agcctctgat cgcctgcgag ttcgacaacc agggcaacgc ccaggcgaag
     2941 tacatcctcg ggccgatgga gctcgacggc acggtcatca acgacgccac cgccgggcgc
     3001 gagcagcagt ccggccagtg gaccgtcaac ctgtcgatga acgccaaggg caccaaggtc
     3061 ttcggcgaga tcagccagcg gctgaacgcc aaccgcatcg cgaacctcag cccgcgcgac
     3121 cagttcgcgt tcgtgctcga cggcaaggtc atctcggcac ccgtcatgca ggccgcgatc
     3181 ctcaacggca agcccagcat ctcgggcaac ttcacgcagg agagcgcgaa gacgctcgcc
     3241 gaccagctca agtacggtgc gctgccgctg agcttcaccg tgcagagctc ggacaccgtc
     3301 tcggcgaccc tcggctcgca gcagctgcag tacggtctga tcgccggtct gatcgggctg
     3361 gggctggtgg cggtctactc actgctcagc taccgagcgc tcggctgggt gatcatggcc
     3421 tcgatcgccg tgatgggcgt gctgacctac atcatcatcg cgatcctcgc gtggcggatg
     3481 ggcttccggc tctcgctcgc cggtgtcgcg ggtctgatcg tgtcgatcgg cttcaccgcc
     3541 gactcgttca tcgtgtactt cgagcgcatt cgggacgagc tgcgcgacgg caggtcgatc
     3601 accggttcga tcgagtacgg ctgggaacgc gccaagcgca cgatctacat cgccaagtcg
     3661 gtcaacatcc tggccgccgt cgtgctgttc atcctcgccg acgcgacggt caagggcttc
     3721 gcgttcacgc tgggcctgac gaccctgatc gacgtgttca tcttcgtgat cttcacgcat
     3781 ccggtcatgc agatcctcgc gcgcacccgc ttcttcggcg gcggtcacag gctgtccggc
     3841 ctgaacgcgg agtcgctggg cgccgtccgc ggtgcccaga ccgagtaccg caccgccgag
     3901 accgcctcga aggggcgtgc ggcgcgcaac aagggcgccc ggggcgaggc cgagcggcgc
     3961 cagacgatcg ccgagcgcaa gcgcgctgag gcactcaccg cgatgggatc caaggatgcc
     4021 gggaaggact ccgacgcctc atgatctcca tgaatgagtt cggcaacaac ctgtactccg
     4081 ggaagacctc cttcccgttc gtcggcaagc gccgcctgtg gttcgtcatc gcgatcctgc
     4141 tggtgctcgg ctccgtgctg gtcccgctcg tgaggccggt gcagttctcc atcgagttca
     4201 ccggcggatc ccagttcatg gtcgtcggcc ccgcgtccac caaccagggc ctggccagcg
     4261 acgccgtgcg ctcagtggtc cccggcgcga cgaccaaggt cgccatcgtc aacaagaagg
     4321 acgtccgcgt ccagaccgac cggatgaccc cgaaccagac gcaggacgtc aaggcggcgc
     4381 tcgcgtcggc cttcgatgtg aaggccgacg aggtctcgga ctccttcatc ggccccgcct
     4441 ggggcgaggg cgtcacgcgg cagtccctgt ggggtctggc gatcttcctg gcgctgacct
     4501 tcctgatcct cggcatctac ttccgcacct ggaagatgtc ggccgcctcg atcctcggac
     4561 tcctggacgt gctggtcatc acggtcggga tctatgccct cgccggcttc gagatctccc
     4621 cggccgcggt gatcgggttc ctgacgatcc tcgcgtactc gctgtacgac accacggtgg
     4681 tgttcgacaa ggttcgcgag aacacccagg aggacgccgt cagcctgcag cgcaccttcg
     4741 gcgagtcggt caacctcgca gtcaaccaga ccctggtccg atcgatcaac acctccgtcg
     4801 tggccgccct tccggtcggc gcgatcctct tcatcggtgc cttctggttc ggtgcggaga
     4861 cgctctcggc gatctcgctg tcgatcttcg tcggcatcct cgtcgcgacc tactccacgc
     4921 tgttcgtggc ggccccgctg tacgcggcgt tccgtcagaa cgagccggac atcctcgcgc
     4981 acgacaagaa ggtgctccag gcgcgcgagc gcgccgcagc gaccacctcc gtgtgagcgg
     5041 acccctgcgg ggaccgggtg cgcccggtcc ccgcgtagga tgaacacatc gcggggacgg
     5101 gagcacagat ggccgattcg caggtcgcat cgcagggatc cagcctgcgc aggctggtgc
     5161 cgcgcatctt ctcccgcgcg ccccgcgtca acgacctgga caacctgatc cgcacggtcc
     5221 gcgccaatca cccccgtggc gatctcccca tcatcgagca cgcgtatcgc gtcgcgaggg
     5281 agaagcacga ggggcagttc cgtcagagcg gcgagcccta catcacgcat ccgctcgcgg
     5341 tcgcgcagat ccttgccgag ctggggctcg ggccgcgcgc gatcgcggcc gccctgctgc
     5401 acgacacggt ggaggacacc ggctacgcac tgagcgagct caccgaggag ttcggcgacg
     5461 aggtcgcgat gctcgtcgac ggcgtcacca agctcgacaa ggtcaagtac ggcgagagcg
     5521 cccaggccga gacggtccgc aagatgatcg tggccatgtc caaggacatc cgcgtgctgc
     5581 tcatcaagct cgccgacagg ctgcacaacg cgcgcacctg ggggttcgtt ccccccgaga
     5641 agtccgcgcg caaggccaag gagacgctgg agatctacgc gcccctggcc aaccggctcg
     5701 gcatccagac catcaagtcc gaactcgagg acctgtcctt cgccgtcctg catccgaaga
     5761 tctacgccga gatcaacagc ctgatcgccc agcgcacccc gcagcgcgag aagtacctgc
     5821 agcaggtgat cgaggcgatc gacgaggacc tgcgcgagct gcggatccgt ggcaaggtgt
     5881 tcggccgtcc caagcagctg tactccgtgt accagaagat ggtcgtgcgc ggacgcgagt
     5941 tcgacgacat ctacgacctc atcggcatcc gcgtgctggt caactcggtg cgcgactgct
     6001 acgcggtgct gggagcgatc cacgcgcgct ggacgccgct gccgggccgc ttcaaggact
     6061 acatcgccac cccgaagttc aacctctacc agtcgttgca caccacggtc atcgggccgt
     6121 ccggccgcac ggtggagatc cagatccgca cgcacgagat gcatcagcag gcggagttcg
     6181 gcgtcgccgc acactggatg tacaaggagc ggatgaacgg cggcaaggtc gagacccgca
     6241 agacggatgc cgacatggcc tggctcgccc acatctccga ctggcaggcc gagaccgcag
     6301 acccgagcga gttcctcgac tcgctccggt tcgagatcgg cgccaaggag gtctacgtct
     6361 tcaccccgaa ggggcgagtc atcggcctgc cggcgggcgc gacgcccgtg gacttcgcct
     6421 acgccgtgca caccgagatc ggccatcgca cgatgggggc caaggtcaac ggccggctgg
     6481 tcccgctgga gtcggagctg cgcagcggtg atgtcgtcga ggtgttcacc tcgaagaacc
     6541 cggatgccgg ccccagccag gactggctga ccttcgtcaa gagcacccgc gcgcggaaca
     6601 agatcaaggg ctggttcacc aaggagcgcc gcgaggaggc ggtcgagcag ggcaaggagt
     6661 ccatcgcccg cgccatgcgc aagcagaacc ttccgctgca gcgactcatg agccaggagt
     6721 cgttcgcgga ggtcgcgcac cagctgcgct acgaggacgt gacggcgctg tacgccgccg
     6781 tcggcgaggg tcatgtctcc acgcagtcgg tgctggagaa ggtcacggcg ctcgtcgcct
     6841 cggagcccga caccagtacg ggtgccatcg agctgcccgg ccacgctccg gctcgggagg
     6901 cgcgctcggg cgactccggc atcctcgtgc gcggcgcggc cgacatcctg gtcaagctcg
     6961 cacgctgttg cacgcccgtc ccgggcgatc ccatcgtcgg cttcgtcacc cgcggcagcg
     7021 gcgtctcggt ccaccgcaag gactgcgtca acgtcaaggc cctcggcagc gaccccgagc
     7081 gcttcgtcga ggtcgagtgg gcgcccacca cgaagagcgt cttccgtgtg cagatccagg
     7141 tcgaggcact ggaccgctcg gggctgctgt ccgatgtgac ccgcgtgctc agcgagcacc
     7201 acgtgaacat cctgtccgcg accgtctcga ccacggacga gcgtctggcg ctgagccgct
     7261 tcgtgttcga gatgggcgac tcggtgcact tggaccgcgt gctgaacgcc gtgcggcgga
     7321 tcgacgccgt ctacgacgtg taccgcgtca cgacgtcctg atcgtcgcga gctccgacat
     7381 cagctgcagg gcgcggcgtc ttccggggcg ccgcaccgtc gccgcgagca tgggctgcag
     7441 cgcgtcgatg agctcggggc tgagcagcag cagcgcgcgc agccagctct cgtcgtcgcc
     7501 gcccggcttc gtcgcggcga acagcagctc ctctgcggtg gcgagaggag tcgtgacctc
     7561 gatgccgccg atcgacagca gctcctcggg cgcggcacga cgctcgtggt agaccacccg
     7621 cggcgagaag ccgacgcgtc cgcgggtagg gccggcccgc cggacgtggt ggatctgcgg
     7681 tggtgcgtca cccgcgccgt ggatccacgc cgccgtcgga ccgcaggcgg ccagatgcga
     7741 ggggatgagg tcgcgcaggc tcgtcgccct ggcatccgcc ccttcgacgg tgtcggcggg
     7801 catgaacccc tcgccgacct cgaagacgac tccgtccagc cgcgcggcgc ccagttcagg
     7861 gagcgagagg cgctcgcccg gccggtagaa gagggcggga tgcatgcaaa gaagtatgag
     7921 ccgtgccgcc cgatgtcggg cgcgcacggc tcatactgtg gataacgcgg atcaggcgcc
     7981 gagtgcgctg agccaggcgc gacgggcggc cagcgcctcc gtcgcctcct tgacggcctt
     8041 cttgtcgccg gccttctccg ccgcggcgag ctcctgctcg agcttctcga tcgcctcgag
     8101 cagctgcgag ctcatgtcgt tcgcgcgcgc cttggtctcg gggttgttct tcttccagtc
     8161 cacgtcctcg cgggacttga gggcctgctc gatggaccgc atgcggtcgt ccagcgcacg
     8221 ctccttctcg cgcgggaaga tgcgcccgac ctcgtcccac tgacgctgga tgcgggtcag
     8281 cagctgacgc gcgcgcttga ggttcggctc gtcggcgacg gccttcgcct cctccaggag
     8341 cgcctcacgg gcctcgatgc gcggtgcgga gtccgcctcc tcggcggcgg cctgctcggc
     8401 ac
//
LOCUS       DATMSX010000396         4365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_44404,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000396 DATMSX010000000
VERSION     DATMSX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44404"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VN041_12330"
     CDS             <1..833
                     /locus_tag="VN041_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWU29859.1"
                     /translation="AYIAGFGATSNLEAGRRWGIPTMGTAAHSWTLLHDSEEDAFRAQ
                     VESLGVSTTLLVDTYDIRTGVETAIRVAGTGLGGVRLDSGDLPIVAGEVRAQLDELGA
                     TETKITVTSDLDEYAIAALAASPVDAYGVGTSVVTGSGYPTAGMVYKLVARQDAGGGW
                     VAVAKASADKGSKGGRKAAFRTLEDGVAVSETISVADGFERVDTAASHPDARALQVVL
                     VENGEIDTAYEGHDGTAAARAHHLRVREELPVRALALSKSDPAIPTHFVDAPEGPTGS
                     "
     gene            complement(931..1491)
                     /locus_tag="VN041_12335"
     CDS             complement(931..1491)
                     /locus_tag="VN041_12335"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29860.1"
                     /translation="MTGSSRIRVRDYEERDWATICRIHDAARLDELRDAAIFRPSPAE
                     LREEWTGPRDGDLSEGRTLVGPDAFLPLVSTFEAEDLFADDVRIAEYDGAVAGFIAGS
                     DDEITWLYVDPAMWRRGIARALVTDHLRRGDGRRVELEVLEGNAARGFYEQLGFVVES
                     TSTGRLAGAEEFWAVGHVMVNSASAS"
     gene            complement(1488..1754)
                     /locus_tag="VN041_12340"
     CDS             complement(1488..1754)
                     /locus_tag="VN041_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HWU29861.1"
                     /translation="MSTPLDSPDQGGVATLDRELEELLREENLEPGDHERFSHYVKKD
                     KILESAITGKPVRALCGKKWTPGRDPEKFPICPTCKEIYESMIA"
     gene            complement(1804..3372)
                     /locus_tag="VN041_12345"
     CDS             complement(1804..3372)
                     /locus_tag="VN041_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29862.1"
                     /translation="MVAHVLRLRLALVAGALRGERLVRSVLAVLVTAAITVAVCLAVL
                     GLGSAPLPTARAIIVFSAAAAFLAFFLGPMLTGTQDQLDPRRFAPFGVDEKRMPWILA
                     LASLVSVPSAALLVVYAFFVAVSITVGGIAWPVAVLAGLVGVISTALIARIGMAISAI
                     ALPERRSRELTVLFWIPIIVVAFPVAAYVSSMEWNGHVPAGVAAVTAAVGFTPLAAPQ
                     AFLFHIAAGEVGAAWGSGLIAVATLAGILALWAYLVRRMLTTTERPYATRERSGLGWF
                     SVLPANAFGAVAARSLVYWMRDRRYIVNILIVPIAGVLTVFPLMVAGVPPWAAALVPV
                     PLMALFFGWLPHNDVSYDSTALWIHIASGVRGLPDRLGRLVPVLLVSIPVLAIAVSVT
                     LAFVGKWELLLPLIGVAAALLLTGLGMSSIASVIAPYAVSRPGDSPFQQPQRSSSHGS
                     WGAAGTFVGALLFSIPTLWLFVLTVVDGDSHAPATFWTGVITGVLVLFGGAAIGGAIF
                     ERSGARLMEFAETT"
     gene            complement(3348..4334)
                     /locus_tag="VN041_12350"
     CDS             complement(3348..4334)
                     /locus_tag="VN041_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005506437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29863.1"
                     /translation="MTKPSPSSVGSPPVDAAPAEEVPAETAAAPAEEVTAEQDAEPVE
                     PAPEPAAEHPAQPEASSEPTRAPRPAPAPATGAEAILIRGLVKRFGDSNAVDGIDLTV
                     PAGSFYGLVGPNGAGKTTTLSIIAGLLRPDAGTVHISGIDQKANPLGAKRVMGVLPDR
                     LRTFDRLTGRQLLHYYGQLRGLQRAVIEKRVADLARAFDLSEALSRVVSDYSAGMTKK
                     LMLAGALIHSPRVLVLDEPFEAVDPVSSSVILDILRSYVAHGGTVILSSHGMDLVEQV
                     CTRVAVIVGGEVLAEGTVDEVRAGQTLAARFVELSGTGGEVEGLEWLHTFSD"
BASE COUNT          711 a         1532 c         1550 g          572 t
ORIGIN      
        1 ccgcctacat cgccggcttc ggcgcgacga gcaacctcga ggcggggcgc cggtggggca
       61 tcccgacgat gggcacggcc gcgcactcct ggacgctgct gcacgacagc gaggaggacg
      121 cgttccgcgc gcaggtggag agcctgggcg tgagcaccac tctgctggtg gacacctacg
      181 acatccgcac cggcgtcgag accgcgatcc gcgtggccgg caccggcctc ggtggggtgc
      241 gcctggactc cggagacctg ccgatcgtcg ccggcgaggt gcgcgcccag ctggacgagc
      301 tcggcgccac cgagacgaag atcacggtca ccagcgacct cgacgagtac gcgatcgccg
      361 ctctggccgc ctcccccgtg gatgcctacg gcgtgggcac ctcggtggtc acgggctccg
      421 gctatccgac cgcgggaatg gtctacaagc tcgtcgcgcg tcaggacgcc ggcggcggct
      481 gggtggccgt cgccaaggca tccgccgaca agggttcgaa gggcgggcgc aaggccgcgt
      541 tccgtacgct cgaggacggc gtcgcggtct ccgagacgat ctcggtggcc gacgggttcg
      601 aacgcgtcga cacggcagca tcccacccgg atgcccgcgc cctgcaggtc gtgctcgtcg
      661 agaacggcga gatcgacacc gcctacgaag ggcacgacgg cacggccgcc gcccgcgcgc
      721 accacctgcg cgtgcgcgag gagctgccgg tgcgcgcgct cgcgctcagc aagtcggacc
      781 ctgcgatccc gacccacttt gttgatgccc cggaggggcc gaccggttcc tgagcgagga
      841 gcgaagcgac gagtcgaagg gcgtcgacgc gccggagggg ccgaccggtt cctgatcccg
      901 gctcgccggc ggcacgagcc ggcgctcagc tcacgaggcg gatgcggagt tgaccatgac
      961 gtgtcccact gcccagaact cctccgcacc tgccagccgc cccgtggacg tcgattcgac
     1021 gacgaagccg agctgctcgt agaagccccg agcggcgttg ccttcgagca cctccagctc
     1081 gacacgtcgc ccgtcaccgc gacggaggtg atccgtcacc agcgctcgcg cgataccgcg
     1141 ccgccacatg gccgggtcga cgtacagcca ggtgatctcg tcgtccgagc ccgcgatgaa
     1201 gccggcgacg gcgccgtcgt actccgcgat ccgcacatca tcggcgaaca ggtcctccgc
     1261 ctcgaacgtg ctcacgagag ggaggaacgc gtccggaccg accagcgtgc gcccttcgct
     1321 caggtcgccg tcgcgggggc ccgtccactc ctcacgcagt tcggcgggcg acggccggaa
     1381 gatcgcggcg tcgcgcaact catccagtcg ggccgcatca tggatgcggc agatcgtcgc
     1441 ccagtcccgt tcctcgtagt cccgcacccg gatccgagag gagccggtca cgcaatcatg
     1501 ctctcgtaga tctccttgca ggtcgggcag atcgggaact tctccgggtc acgtcccggc
     1561 gtccacttct tgccgcagag cgcccgcacc ggcttgccgg tgatggccga ctcgaggatc
     1621 ttgtccttct tcacatagtg cgagaagcgc tcatggtcgc cgggctcgag gttctcctcg
     1681 cggaggagct cttcgagttc gcgatcaagt gttgccacgc caccctgatc ggggctgtcc
     1741 agcggagtac tcatgggccg attctagccg tcatgagccc tccggggccc ggttcctgcg
     1801 gtctcaggtg gtctcggcga actccatcag gcgggctccg ctgcgctcga agatcgcacc
     1861 gccgatcgcc gcgccgccga acagcacgag cactccggtg atgactcccg tccagaacgt
     1921 cgccggggcg tgactgtcgc catccacgac ggtgagcacg aacagccaca gcgtggggat
     1981 gctgaagagg agcgccccga cgaacgtgcc tgccgcaccc caggacccgt gcgaggacga
     2041 gcgttgcggc tgctggaacg ggctgtcgcc cgggcgcgac accgcgtacg gcgcaatgac
     2101 cgaggcgatg ctcgacatgc cgagcccggt gagcagaagc gcggcggcca caccgatgag
     2161 cggcaggagc agttcccact tcccgacgaa cgccagggtc acggacacgg cgatcgccag
     2221 cacggggatc gacacgagca gcacgggcac gagccggccg agcctgtcgg gcagcccgcg
     2281 gacgccgctc gcgatgtgga tccacagcgc ggtggagtcg taggagacgt cgttgtgcgg
     2341 cagccagccg aagaacagcg ccatgagcgg caccggcacg agcgcagccg cccacggcgg
     2401 gactccggcc accatgagcg ggaacaccgt gagcactccg gcgatcggca cgatgaggat
     2461 gttcacgatg tagcggcgat cgcgcatcca gtagaccagg ctgcgggccg cgacggcacc
     2521 gaacgcgttg gccggcagca cggagaacca ccccaggccg gagcgctcgc gggtcgcgta
     2581 cggacgctcg gtggtcgtca gcatccgccg gacgagatac gcccagagcg ccaggatgcc
     2641 ggccagcgtc gccactgcga tgaggccgct gccccacgcc gccccgacct cgccggctgc
     2701 gatgtggaac aggaacgcct gcggggcggc cagcggcgtg aagcccaccg ccgcggtcac
     2761 ggcggcgacc ccggcgggta catggccgtt ccactccatc gacgagacgt acgcggcgac
     2821 cgggaacgcg acgacgatga tggggatcca gaagagcacc gtgagctcgc gggagcggcg
     2881 ctcgggcagc gcgatggcgc tgatcgccat gccgatgcgg gcgatcagcg cggtggagat
     2941 cacgccgacc agtccggcca gcacagcaac aggccacgct atgcctccga ccgtgatcga
     3001 gacggcgacg aagaatgcat agacgacgag gagcgcggcg ctcgggacgc tgaccaggga
     3061 ggcgagcgcc aggatccacg gcatccgctt ctcgtccaca ccgaagggcg cgaagcgtcg
     3121 aggatccagc tggtcctgcg tgccggtgag catcgggccc aggaagaacg cgaggaacgc
     3181 ggcggcggcg ctgaacacga tgatcgcgcg cgcggtgggc agcggtgcgc tgcccaggcc
     3241 gagcacggcc aggcacacgg cgacggtgat cgcagccgtg accagcactg cgagcacgct
     3301 gcgcacgagt cgttcgccgc gcagagcgcc cgcgaccagc gcgagcctca gtcggagaac
     3361 gtgtgcaacc actccagtcc ctccacttcg ccacctgtgc cggagagctc gacgaagcgc
     3421 gcggcgagcg tctggcccgc gcgcacctcg tccacggtcc cctcggcgag cacctcaccg
     3481 ccgacgatga ccgcgacgcg cgtgcacacc tgctccacca ggtccatgcc gtggctggag
     3541 aggatgaccg tgccgccgtg ggcgacgtag gagcgcagga tgtcgaggat cacgctcgac
     3601 gagaccgggt cgacggcctc gaacggctcg tcgagcacga gcacgcgcgg cgagtggatc
     3661 agggcgcccg cgagcatgag cttcttggtc atgccggccg agtagtcgga gaccacgcgg
     3721 ctcagcgcct cgctcaggtc gaacgcgcgt gccagatcgg cgacgcgctt ctcgatgacc
     3781 gcgcgctgca ggccccgcag ctgaccgtag tagtgcagca gctggcggcc ggtgaggcgg
     3841 tcgaacgtgc gcagccggtc cggaagtacg cccatcacgc gcttcgcgcc gagcgggttc
     3901 gccttctggt cgatgccgct gatgtgcact gtgccggcgt cgggacgcag cagccccgcg
     3961 atgatcgaca gcgtggtggt cttgcccgcg ccgttcggcc ccaccagtcc gtagaacgaa
     4021 ccggcgggca cggtcaggtc gatgccgtcg accgcgttcg aatcgccgaa ccgcttgacc
     4081 aggccgcgga tgaggatggc ctccgcaccg gtggccggag cgggtgcagg gcgcggagcg
     4141 cgtgtgggct ccgacgacgc ctcgggctgc gcgggatgct ctgcagcggg ctcgggggcc
     4201 ggctcgacgg gctcggcgtc ctgctcggcc gtgacttctt cagccggcgc ggctgccgtc
     4261 tcggccggga cttcttcagc cggcgccgcg tcgaccggcg gcgatccgac agaggaaggc
     4321 gacggcttcg tgatggcttc gggcgtcgcc gcggaggcgg ccgcc
//
LOCUS       DATMSX010000397         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_157968,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000397 DATMSX010000000
VERSION     DATMSX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157968"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VN041_12355"
     CDS             complement(<1..1381)
                     /locus_tag="VN041_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU29864.1"
                     /translation="MSDAASGEGIFPDAAFLILSSRLIPGLDGGYTIATAARARQMAE
                     HGADVQVLTVDPADAEAHAAHRAEFRRLGMLPEGVPLRNLFDEASAPGGGAAPWLRAA
                     SETELRRPDHVSSTAPVGDVDIRLTRQEGVRSGEEADSYRELTDAEGRVYAALPVIQG
                     NPDWHLSTAAVRVYDETGETVGTLDGFGGLYRAWLDHLVEGSDRPVVVICESRQLGEL
                     LAGWSNPRVRIVHAIHTMHLEPPYTPNSPLNALWTRWFAVSDRFDAVIWPTQAQRDDV
                     QSRFGGDAVNLVSPHGIRLAAEGDHAREPGLVVSVSRLAPGKRIDHLVRAFLAADVPG
                     TRLEIWGDGPSTASVAELIATLGAGDRVALMGRTDDPAAVLRRASLMVTATAYEGQGL
                     SIVEALAAGCPVVSYDVRYGPGDALRRGGGLLVPASDERMLADAIRQVLTDAPLRERL
                     AAEAPVAASA"
     gene            complement(1387..2973)
                     /gene="guaA"
                     /locus_tag="VN041_12360"
     CDS             complement(1387..2973)
                     /gene="guaA"
                     /locus_tag="VN041_12360"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWU29865.1"
                     /translation="MTEQTETQQRPALVVDFGAQYAQLIARRVREAGVYSEIVPHTAT
                     AEEIAAKNPVALLLSGGPSSVYEEGAPKLDPAVFELGVPTLGICYGFQYMAQTLGGEV
                     ANTGLREYGATDAHIPENGSSLLAGQPAEQNVWMSHGDQVARAPEGFEVLATTDATKV
                     AAFANEERKLYGVQWHPEVKHSDHGQEILENFLHKSAGLSADWNSDNVIAEQIERIRE
                     QVGDARVISALSGGVDSAVSTALVHKAIGDQLTAVFVDHGLLRKGEREQVEKDYVEST
                     GVRLITVDAVDTFLGHLAGVTDPETKRKIIGREFIRAFEQVQRDLVEEAKATGGAPVK
                     FLVQGTLYPDVVESGGGAGTANIKSHHNVGGLPEDLDFELVEPLRALFKDEVRAIGRE
                     LGIPEAIVGRQPFPGPGLGIRIIGEVTRERLDILREADAIAREELTKAGLDQTIWQCP
                     VVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRLPYDVLSRISNRITNEVS
                     EVNRVVLDVTSKPPATIEWE"
     gene            complement(2970..3482)
                     /locus_tag="VN041_12365"
     CDS             complement(2970..3482)
                     /locus_tag="VN041_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HWU29866.1"
                     /translation="MPEPKIASFPAIRGALKFYQIASIITGVMLLLLLTEMIVKYSPI
                     HHELFLGGSGGFLWFADVIVGDGCQWYSLFVPGGMGCKLTSVGDGVNLSLGILVAHGW
                     FYVVYLFACFRVWSLMRWPFRRFILLALGGVIPFLSFFMELRVSREVKNYLATREAAD
                     ASLPAPEGAR"
BASE COUNT          543 a         1230 c         1288 g          515 t
ORIGIN      
        1 aggcggatgc cgccacggga gcctccgcgg ccaggcgctc gcgcagcggg gcgtccgtga
       61 gcacctggcg gatggcatcc gccagcatcc gctcgtcgct ggccgggacg agcagaccgc
      121 ctccgcggcg cagggcatca ccggggccgt agcgcacgtc gtacgacacg acggggcacc
      181 cggctgcgag cgcctccacg atggacaggc cctgcccctc gtacgccgtg gcggtgacca
      241 tgagcgaggc gcggcggagc actgcggccg gatcgtccgt gcggcccatc agcgcgacgc
      301 ggtcccccgc gccgagggtc gcgatcagtt cggcgacgga tgctgtggac gggccgtcgc
      361 cccagatctc caggcgcgta ccggggacgt ctgcggcgag gaaggcgcgc acgaggtggt
      421 cgatgcgctt gccgggggcc agccgcgaga cgctgacgac cagtcccggc tcgcgcgcgt
      481 ggtcgccctc cgcggcaagg cggatgccgt gcggcgagac caggttcacg gcgtcaccgc
      541 cgaaccggga ctgcacgtcg tcacgctgcg cctgcgtcgg ccagatcacg gcgtcgaacc
      601 ggtcgctgac cgcgaaccag cgtgtccaca gggcgttcag cggagaattc ggagtgtacg
      661 gcggctcgag gtgcatggtg tggatcgcgt gcacgatgcg caccctcggg ttcgaccagc
      721 ccgcgagcag ctcgccgagc tggcgcgact cgcagatcac gacgaccggg cggtcggacc
      781 cctcgacgag atgatcgagc caggcgcggt acagtccgcc gaagccgtcg agcgtgccga
      841 cggtctcccc ggtctcgtcg tacacccgca cggcggccgt gctcaggtgc cagtccgggt
      901 tcccctggat caccggcaga gccgcgtata cgcgcccctc ggcatccgtc agctcgcggt
      961 aggagtcggc ctcctccccc gagcgcaccc cctcctgacg agtgagccgg atgtcgacgt
     1021 cgccaaccgg tgcagtcgac gagacgtggt ccggtcggcg gagttccgtc tcggatgccg
     1081 cgcgcagcca gggggccgcc ccgccgccgg gggccgatgc ctcgtcgaag aggttccgaa
     1141 gggggacgcc ctcgggcagc atgccgaggc ggcggaactc cgcgcggtgc gcggcgtgcg
     1201 cctcggcatc ggcgggatcc accgtgagca cctgcacgtc ggcgccgtgc tcggccatct
     1261 ggcgggcgcg cgcagcggtg gcgatcgtgt aaccgccgtc gagcccgggg atcagcctgc
     1321 tcgacaggat gagaaacgcc gcgtccggga agatgccctc ccccgacgcg gcgtcgctca
     1381 cgatgcttac tcccactcga tcgtcgccgg cggcttcgag gtgacgtcga gcacgacgcg
     1441 gttgacctca ctgacctcgt tggtgatgcg gttcgagatc ctggacagca cgtcgtaggg
     1501 caggcgagtc cagtcggcgg tcatcgcatc ctcggacgag accggacgca gcacgatcgg
     1561 gtgaccgtag gtgcggccgt cgccctgcac gcccaccgag cggacgtcgg cgagcagcac
     1621 gaccgggcac tgccagatcg tctggtccag accggccttc gtcagctcct cgcgcgcgat
     1681 cgcgtcagcc tcgcgcagga tgtcgaggcg ctcgcgggtc acctcgccga tgatgcggat
     1741 gcccaggcca gggccgggga agggctggcg gccgacgatc gcctcgggga tgccgagctc
     1801 gcggccgatc gcgcggacct cgtccttgaa cagggcgcgc agcggctcga cgagttcgaa
     1861 gtcgagatcc tcgggcaggc cgcccacgtt gtggtgcgac ttgatgttcg cggtgcccgc
     1921 tccgccgccg gactcgacga catccgggta cagggtgccc tgcaccagga acttcacggg
     1981 cgcaccgccg gtggccttgg cctcctccac caggtcgcgc tgcacctgct cgaaggcgcg
     2041 gatgaactcg cggccgatga tcttgcgctt ggtctcgggg tcggtgacgc cggcgaggtg
     2101 gcccaggaag gtgtccacgg cgtccaccgt gatcaggcgc acgccggtgg actcgacgta
     2161 gtccttctcg acctgctccc gctcgccctt gcgcagcaga ccgtggtcga cgaacaccgc
     2221 ggtgagctgg tcgccgatcg ccttgtgcac gagagccgtc gacacggccg agtcgacgcc
     2281 gccggagagg gcggagatga cgcgagcgtc accgacctgc tcgcggatgc gctcgatctg
     2341 ctcggcgatc acgttgtcgc tgttccagtc tgcggacagc ccggcgctct tgtgcaggaa
     2401 gttctcgagg atctcctggc cgtggtcgga gtgcttcacc tcggggtgcc actgcacgcc
     2461 gtagagcttg cgctcctcgt tcgcgaaggc ggcgaccttg gtggcgtcgg tggtggcgag
     2521 cacctcgaag ccctcgggtg cgcgggcgac ctggtcgccg tggctcatcc acacgttctg
     2581 ctctgcgggc tggccggcga gcagcgagct gccgttctcg gggatgtgcg cgtcggtcgc
     2641 gccgtactcc cgcagaccgg tgttggcgac ctcgccgccc agggtctgcg ccatgtactg
     2701 gaagccgtag cagatgccca gcgtcggcac accgagctcg aacacggccg ggtcgagctt
     2761 aggggcgccc tcctcgtaga ccgaggacgg gccgccggag agcagcagcg cgaccgggtt
     2821 cttcgcggcg atctcctcag cggtcgccgt gtgcggcacg atctcgctgt acacgcccgc
     2881 ctcgcggacg cggcgtgcga tcagctgcgc gtactgcgcg ccgaagtcga cgacgagcgc
     2941 ggggcgctgc tgggtctcgg tctgttcggt caacgggcac cttccggggc ggggagggag
     3001 gcgtcggcgg cctcacgggt ggcaaggtag ttcttcacct cgcgcgagac gcgcagctcc
     3061 atgaagaacg acaggaacgg gatcacaccg ccgagggcga gcaggatgaa gcgccggaac
     3121 ggccagcgca tcaggctcca cacccggaag caggcgaaca ggtacacgac gtagaaccag
     3181 ccgtgtgcga cgaggatgcc cagcgacagg ttgacaccgt cgccgacgct ggtgagcttg
     3241 cagcccatgc caccgggaac gaacagcgaa taccactggc atccgtcgcc cacgatgacg
     3301 tcggcgaacc acaggaatcc gccggatccg ccgaggaaca gctcatgatg gatgggcgag
     3361 tacttcacga tcatctcggt cagcagcagg agcagcatga cgccggtgat gatggacgcg
     3421 atctgataga acttcagcgc gccgcggatc gccgggaaac tggcgatttt cggctcgggc
     3481 atggatacca gtctagcggc cgctccgagc gcgcccgccc ccgccccgag ccgtgcgggc
     3541 gacaggcccc gggtacgcgg agaggccgcc gggatc
//
LOCUS       DATMSX010000398         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_24839,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000398 DATMSX010000000
VERSION     DATMSX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24839"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VN041_12370"
     CDS             complement(<1..313)
                     /locus_tag="VN041_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HWU29867.1"
                     /translation="MSIPRPSAQQLRRLAGDGGYQRGLDYARRGHVERTSWDAATETL
                     TADVYGSEPAPYRCRIRFNGGEIAGTACSCPMQRGCKHVVAAMLVAPEKEARERIAVE
                     RR"
     gene            complement(374..784)
                     /gene="nusB"
                     /locus_tag="VN041_12375"
     CDS             complement(374..784)
                     /gene="nusB"
                     /locus_tag="VN041_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWU29868.1"
                     /translation="MSARTKARKRALDILFSADVRGEEVSVSLAAAAARAASELSRQA
                     SWLYARDIVDGIIDHHDEIDEQITTHSRDWKLERMPAVDRALLRIGTWEILFNDEVPT
                     AVAIDEAVELAKELSTEDSGAFVHGVLARIDRAR"
     gene            complement(786..1349)
                     /gene="efp"
                     /locus_tag="VN041_12380"
     CDS             complement(786..1349)
                     /gene="efp"
                     /locus_tag="VN041_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWU29869.1"
                     /translation="MASTADIKNGVVLSIDGQLWSVIEFQHVKPGKGGAFVRTKLKNV
                     VSGKVVDRTYNAGAKIDIENVDRRDFQYLYTDGDSFVFMDVEDYDQLTVGAATVGDAA
                     HFMLENQQVQIALNNGNPLYIELPASVVLEVTYTEPGLQGDRSSAGTKPATLETGYEI
                     QVPLFLEQGTKVKVDTRTGGYLGRVND"
     gene            complement(1426..2295)
                     /locus_tag="VN041_12385"
     CDS             complement(1426..2295)
                     /locus_tag="VN041_12385"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU29870.1"
                     /translation="MLASEDRFDAVPAEPNGTAVLLLAGSSGRVERERAELLSTTGAR
                     VRAIRWFGGADQRPSPHEVPLELFAGQIGELRRDADRVVLFGTSFGAEAVLTTAAHAT
                     VDGVIAVAPSSVVWAGAHDGAWSSHWTLGSRPLPFVAFDVSWTPQSDPPAFVSLYEAS
                     LRLDAAETDAARIPVEKIAGEVLLIAGGDDQVWPSVRFATEIEAVRTDHDLPTTIVTH
                     PGAGHRLLLPGEPPAAGGVRMLRGGTPEADAELGARAWPEIRRLLSGPAQDRVGEAGK
                     QLCGEHGPNPGIR"
     gene            complement(2301..2744)
                     /locus_tag="VN041_12390"
     CDS             complement(2301..2744)
                     /locus_tag="VN041_12390"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWU29871.1"
                     /translation="MSTRRLLLVNGPNLNLLGTREPEVYGTATLADVEQLVAEVADGL
                     GYEVRAVQSNHEGVLIDAIHAVRGDCAGIVINPGGLTHTSVSLRDALTGVGMPFAEVH
                     ISDVYARESFRHFSYLEDVASVRVVGKGIDGYAEAVRELVAQLGE"
     gene            complement(2741..3820)
                     /gene="aroB"
                     /locus_tag="VN041_12395"
     CDS             complement(2741..3820)
                     /gene="aroB"
                     /locus_tag="VN041_12395"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWU29872.1"
                     /translation="MTVKTISVTGQAAYDITVGRDILDRVRTALDPAVKKVLVVHPPT
                     LANRAAELRDRLVADGGVQVLLAEIPDAEAGKRIEVAAFCWQVMGQADFTRSDAVIGY
                     GGGAVTDLAGFVAATWLRGVQVVQVPTTVLGLVDAAVGGKTGVNTNEGKNMVGAFWAP
                     QAVIGDLDELASLSANEATAGYAEVVKAGFIWAPEILDIIEADPARAVDTSTDEFRRT
                     IELAIEMKAKVVSDDFREAGLREILNYGHTLGHAIEHTERYQWRHGAAISIGMMYAAE
                     LSRLAGRLPDAAVDRHRNVLESLGLPTTYRAGAWNTLLATMQRDKKARGGMLRFIVLD
                     DIAKPTVLQAPDESLMFAAYQEVGA"
     gene            complement(3817..4350)
                     /locus_tag="VN041_12400"
     CDS             complement(3817..4350)
                     /locus_tag="VN041_12400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWU29873.1"
                     /translation="MMSSPERADPPLTLVLVGPMAAGKTSVGRKVARLLEVPFSDTDK
                     RIAAVHGPIPQIFAEHGEPYFRELERAAVAATVADGGVVSLGGGAVTDAATRALLTRH
                     PVVFLTVSPEAVSRRIGGTGRPLLTGDDDPLERWRRIFDERRGWYEEVADLTVDTSRT
                     PMRMLAERIAAWRKEQE"
     gene            complement(4334..>4599)
                     /locus_tag="VN041_12405"
     CDS             complement(4334..>4599)
                     /locus_tag="VN041_12405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWU29874.1"
                     /translation="LRTIDVATGEEATAHHQRSDVCAVPAAGVVAEAMVAVELANSVL
                     EKFGGDSIRETRRNLEGYLAGIPEALRTAPASEAALLAHDELS"
BASE COUNT          657 a         1641 c         1569 g          732 t
ORIGIN      
        1 ggcggcgttc gaccgcgatc cgctcgcggg cctccttctc gggtgcgacg agcatcgccg
       61 cgacgacgtg cttgcagccg cgctgcatgg ggcaggagca cgcggtgccg gcgatctctc
      121 cgccgttgaa gcggatgcgg caccggtacg gtgcgggctc gctgccgtac acatcggccg
      181 tgagcgtctc ggtcgcagca tcccacgatg tgcgctccac gtggccgcga cgcgcgtagt
      241 ccaggccgcg ctgatagccg ccgtcaccgg cgagccggcg caactgctgt gcactgggcc
      301 ggggaatgct catctcagca tccttcccgg cagcaccgac actccgcccg gttcagcggg
      361 gatcctgcgc aactcagcgt gcgcggtcga tgcgggcgag cacgccgtgc acgaacgcgc
      421 cggagtcctc ggtggacagc tccttggcca gctccactgc ctcgtcgatc gcgacggcgg
      481 tgggtacctc gtcgttgaag aggatctccc aggtgccgat ccgcagcagg gcgcgatcga
      541 ccgcgggcat ccgctcgagc ttccagtcac ggctgtgcgt ggtgatctgc tcgtcgatct
      601 cgtcgtggtg gtcgatgatc ccgtcgacga tgtcgcgggc gtacagccac gacgcctggc
      661 gggagagctc gctcgctgcg cgtgcggcag ccgcggcgag ggacaccgac acctcttccc
      721 cgcgcacgtc agcggagaag aggatgtcga gggcgcgctt gcgcgccttg gtgcgtgcgc
      781 tcaactcagt cgttgacccg gccgaggtag ccaccggtgc gggtgtcgac cttgaccttg
      841 gtgccctgct cgaggaacag cggaacctgg atctcgtagc cggtctcgag cgtggcgggc
      901 ttggtgccgg ccgacgagcg gtcgccctgc aggcccggct cggtgtaggt gacctcgagc
      961 acgacggacg ccggcagctc gatgtacagc gggttgccgt tgttcagagc gatctgcacc
     1021 tgctggttct cgagcatgaa gtgcgcggcg tcgccgacgg tcgcggcacc gacggtgagc
     1081 tggtcgtagt cctcgacgtc catgaagacg aagctgtcgc cgtcggtgta gaggtactgg
     1141 aagtcgcgac ggtcgacgtt ctcgatgtcg atcttggcgc ctgcgttgta ggtgcggtcg
     1201 accaccttgc cggagacgac gttcttcagc ttggtgcgca cgaacgcacc gcccttgccg
     1261 ggcttgacgt gctggaactc gatgacgctc cacagctgtc cgtcgatcga gagcacgacg
     1321 ccgttcttga tgtctgcagt ggatgccatg ctggtgcgag tccgttcgtt tcgaaggttc
     1381 aatggccgag cgcggatgcg cgcaggaagt ggccaaggat tcatcttatc ggatgccggg
     1441 gttcggcccg tgttccccgc acagttgctt gccagcttcg cccacgcggt cctgggcggg
     1501 tccagacagc agccgtcgga tctccggcca cgcccgggca cccagttcgg catccgcttc
     1561 cggagtgccg ccgcgcagca tccgaacacc gcccgccgcc gggggctccc cgggcagcag
     1621 cagacgatgt cccgccccgg gatgggtgac gatcgtcgtg gggaggtcgt gatcggtccg
     1681 caccgcctcg atctccgtcg cgaaccggac gctcggccag acctgatcgt cgccgcccgc
     1741 gatcagcagc acctcgcccg cgatcttctc cacgggtatc cgcgccgcgt cggtctcggc
     1801 ggcatccagc cggagactcg cctcgtacag ggagacgaaa gcaggcggat ccgactgcgg
     1861 ggtccaggac acgtcgaagg cgacgaacgg cagaggccgg ctgccgagcg tccagtgcga
     1921 ggaccacgcc ccgtcgtgcg cgcctgccca gaccaccgac gagggcgcga ctgcgatcac
     1981 gccgtcgacc gtggcatggg ctgccgtggt cagcaccgcc tccgcgccga acgatgtgcc
     2041 gaacagcacc acccggtccg cgtcgcggcg cagctccccg atctgccctg cgaagagctc
     2101 cagcggcacc tcgtgcgggg agggccgttg gtctgctccg ccgaaccacc ggatcgcgcg
     2161 cacgcgtgcg ccggtcgtcg acagcagttc cgcgcgctcc cgctcgacgc gcccgctgga
     2221 ccccgcgagc agcagcacgg cggtgccgtt cggctcggcg ggaacggcgt cgaagcgatc
     2281 ctcggatgcg agcatggcga ctactcgccg agctgtgcga ccagctcccg cacggcctcg
     2341 gcatagccgt cgatgccctt gccgaccaca cgcaccgagg cgacgtcctc gaggtacgag
     2401 aagtgccgga aggactcccg cgcgtacacg tcggagatgt gcacctcggc gaacggcatg
     2461 ccgacaccgg tcagcgcgtc acgcagcgac accgaggtgt gggtcagacc gcccgggttg
     2521 atgacgatgc ccgcgcagtc cccgcgcacg gcgtggatcg cgtcgatcag cacgccctcg
     2581 tggttgctct gcacagcgcg cacctcgtag ccgaggccgt cggcgacctc ggccaccagc
     2641 tgctcgacat cggcgagcgt cgcggtgccg tacacctccg gctcgcgggt gccgagcaga
     2701 ttcaggttcg ggccgttgac gagcaggagg cgccgagtgc tcacgctccg acctcctggt
     2761 aggcggcgaa catcagggac tcgtccggcg cctgcagcac ggtgggcttg gcgatgtcgt
     2821 ccagcacgat gaaccgcagc atgccgccgc gcgccttctt gtcgcgctgc atggtcgcca
     2881 gcagcgtgtt ccacgctccc gcccggtagg tggtcggcag gcccagcgat tcgagcacgt
     2941 tccgatgccg gtcgacagcg gcgtcgggca ggcgtccggc cagacgcgac agctccgccg
     3001 cgtacatcat gccgatcgag atcgccgcgc cgtggcgcca ctggtagcgc tcggtgtgct
     3061 cgatcgcgtg cccgagcgtg tgcccgtaat tgaggatctc cctcagcccc gcctcgcgga
     3121 agtcgtccga gacgaccttc gccttcatct cgatcgcgag ctcgatcgtg cggcggaact
     3181 cgtccgtgga ggtgtcgaca gcgcgcgccg ggtcggcttc gatgatgtcc aggatctccg
     3241 gtgcccagat gaagccggcc ttcacgacct ccgcgtaacc ggcggtcgcc tcgttcgcgc
     3301 tcaggctggc cagctcgtcg aggtcgccga tcacggcctg cggcgcccag aacgcaccga
     3361 ccatgttctt gccctcgttg gtgttcacgc cggtcttgcc gcccacggcc gcatccacca
     3421 ggccgagcac cgtggtcggc acctgcacca cctgcacgcc acgcagccag gtcgcggcca
     3481 cgaatccggc gaggtcggtg accgcgccgc cgccgtaccc gatcacggcg tccgagcggg
     3541 tgaagtcggc ctggcccatg acctgccagc agaacgccgc gacctcgatg cgcttgcccg
     3601 cctcggcgtc cgggatctcg gcgagcagca cctgcactcc cccgtccgcc accaggcggt
     3661 cgcgcagctc tgccgcgcgg ttcgcgagcg tcggcgggtg caccacgagc accttcttga
     3721 ccgccgggtc cagggccgtc ctgacccggt cgaggatgtc gcgccccacg gtgatgtcgt
     3781 aggcggcctg tccggtcacg ctgatggtct tgacggtcat tcctgttcct tcctccaggc
     3841 ggcgatcctc tccgccagca tccgcatcgg cgtacgggag gtgtcgacgg tgagatccgc
     3901 gacctcctcg taccagccgc ggcgctcgtc gaagatcctc cgccatcgtt ccagcgggtc
     3961 atcgtcgccg gtgagcagcg gccgtccggt gcccccgatg cggcgggaca ccgcctccgg
     4021 ggacaccgtg aggaacacga ccggatgccg cgtcagcagc gccctcgtcg cggcatccgt
     4081 cacggcgcca cccccgagcg acaccacacc gccgtccgcc accgtggcgg cgaccgctgc
     4141 acgctccagt tcgcggaagt acggctcgcc gtgctctgcg aagatctgcg gaatcggtcc
     4201 gtgcacggcg gcgatccgct tgtcggtgtc gctgaagggc acctcgagca gtctcgcgac
     4261 cttgcgaccg acgctcgtct tgcccgcggc catcgggccg acgagcacca gtgtcagcgg
     4321 cggatcagcc cgctcaggag agctcatcat gggcgagcag cgccgcctcc gatgcgggag
     4381 cggtgcgcag ggcctccggg atgccggcga ggtagccctc caggttgcga cgcgtctcgc
     4441 ggatgctgtc gcccccgaac ttctccagca ccgagttggc gagctcgacg gcgaccatgg
     4501 cctcggcaac gacaccggct gcgggcacgg cgcacacgtc ggagcgctgg tggtgcgccg
     4561 tggcctcctc gccggtcgcg acgtcgatcg tgcgcagcg
//
LOCUS       DATMSX010000399        20593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147710,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000399 DATMSX010000000
VERSION     DATMSX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147710"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VN041_12410"
     CDS             <1..815
                     /locus_tag="VN041_12410"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWU29875.1"
                     /translation="TGAPFALAGAARVTSTPHYDDVSQLIGRLASEHTVNGLHVHVEV
                     TGDEERVRALLRLREALPLLLALSANSPFAFGVPAGLASWRSTLIRRLPVSWGPPIFT
                     DADHYRRTVDRLVGIGLLPARSSVSWAVRLSEQYDTVETRVADAQLEVEDTLLLAALT
                     RAIVLADDVIPTSSAPDDLDASLWLAARHGTQARFCTADGGSESAWAAMERVLAGIRP
                     VLDELGDAEFVDDRLAYLRAEGTGAERQLRAHASGGVEALARLFGAPSQPLA"
     gene            complement(800..1819)
                     /gene="trpS"
                     /locus_tag="VN041_12415"
     CDS             complement(800..1819)
                     /gene="trpS"
                     /locus_tag="VN041_12415"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWU29876.1"
                     /translation="MPELERRIIDDPGSFRVLTGERPTGSLHLGHHFGTIRERVRLQA
                     LGVETFIILADYQVITDRDTAANVADHVRGAVLDYLAAGLDPERTTIFTHSAVPALNQ
                     LLLPFLSLVSEAELHRNPTVKAEHAASGRALSGLLLTYPVHQAADILFCKGNLVPVGK
                     DNLPHVEITRTIARRFNERYGEVFPEPEAMVTETPEIPGLDGRKMSKSYGNAIALSMS
                     EDETADAVRRTVTDSDRVITYDPEGRPGVSGLLSTAALCTGSTPEAVAERLGDAGSGA
                     LKAFTTDAVNAFLSGHRARRAELAADPGLVDRVLREGNVRANAIAEATLAEVRDHMGM
                     GYANG"
     gene            2052..2990
                     /locus_tag="VN041_12420"
     CDS             2052..2990
                     /locus_tag="VN041_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWU29877.1"
                     /translation="MQTILGANGQIAVELARDLNRRGSALRLVSRNPREVNAGDELTR
                     ADLLDAAQTAAAVKGSEVVYFTAGLPANAELWERQFPTMLRNALDASRAAGAAFAYFD
                     NTYMYPQDDRVQTEETPFAPLGRKGRVRAAMAEMVLDEMARGDIPVLIARAPEFYGPG
                     RTQSFTNSLVLDPLLAGRKARVPVRDDRARTLIWTPDASRGLAELGTTPDAFGQTWHL
                     PVHPDRLTYRELIALAGEVFGIEPQYSVVPKWMLSAAGVFSAEARVIRELLPRYQYDN
                     LFDSTKFRTRFPAFAITDHREGLAVIRDERRASDGR"
     gene            2980..3564
                     /locus_tag="VN041_12425"
     CDS             2980..3564
                     /locus_tag="VN041_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29878.1"
                     /translation="MDDESTPESRRSGYHHGDLRASLLSTAMEMLEAGESFSLRAVAR
                     EAGVSPTAPYRHFADREALESALAVQGFDDLRRRLSADDARASSVEELVGFAVTYVAF
                     ALERPALFSLMFGRECDDSDDERVRAAGDLHALLADSLGDVFPDRDPGPLATALWSLT
                     HGLAFLHLDGKLSAASADEVAARVRTSFAALGFS"
     gene            3576..4265
                     /locus_tag="VN041_12430"
     CDS             3576..4265
                     /locus_tag="VN041_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWU29879.1"
                     /translation="MTARILHVVTNVGHYDDPAHPTGLWLSELAHAWEVFEKQGFPQT
                     IVSPAGGASPLEPRSLRFPNYDRIARAWHEDPARMALLKNTSSPDAVDAADFDAIYFT
                     GGHAVMYDFPGSEGLRRITREIFERGGVVSSVCHGYCGLLDTTLSDGSHLIAGRRMTG
                     FSWREEELAKVDELVPYNAEEAARERGALYEKALIPFVSNVVVDGRLVTGQNPGSAKA
                     TARKVVEVLGG"
     gene            complement(4362..5204)
                     /locus_tag="VN041_12435"
     CDS             complement(4362..5204)
                     /locus_tag="VN041_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU29880.1"
                     /translation="MGYINVGTENTTPIELYYEDQGEGQPVVLIHGYPLNGHSWELQT
                     RELLAQGYRVITYDRRGFGNSSKVSVGYDYDTFAADLNTVLETLDLRDVVLVGFSMGT
                     GELARYVATYGHDRVAKLAFLASLEPFLVERDDNPEGVPQEVFDGIVEAARTDRYSWF
                     TQFYNDFYNLDENLGSRISQQVVTANWNLSVTSAPAAAYAVVPAWIEDFRGDVEAVRL
                     AGKPTLILHGTKDNILPIDATARRFHEAVPAADYVEIEGAPHGLLWTHADDVNAALKA
                     FLAK"
     gene            5300..5878
                     /locus_tag="VN041_12440"
     CDS             5300..5878
                     /locus_tag="VN041_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29881.1"
                     /translation="MTETDAREQILTAAEDLYYRKGYAAVGMDELRTAAGVSLRRLYA
                     LFPSKIDVIAAVLQRKHDEWEAALEAAVTAAGDSPRDRLLAVYAHLERWFCDDDFRGC
                     AFINAFGELGGNDPEIARVVREHKAAFQERMAVLADEAGVPREVAFQLSILAEGAQTT
                     AAIAAAPQAARHARAAAEILIDASLAPHPSRA"
     gene            complement(5849..6340)
                     /locus_tag="VN041_12445"
     CDS             complement(5849..6340)
                     /locus_tag="VN041_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWU29882.1"
                     /translation="MTTSSLQLPARSGTGIRVFVMLASVATILTLTLAPRALVGPARG
                     LFMRFAHLVANPVVEPMTYAQVESTLNALMFVPLGAALAILLSRRLWVLAPMIGFVLS
                     IAVETLQSRIPGRVPDLGDVVWNSLGALAGAIVAGVLRLVALGLRRLRTRVSSRAGRM
                     RRE"
     gene            6451..7047
                     /locus_tag="VN041_12450"
     CDS             6451..7047
                     /locus_tag="VN041_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HWU29883.1"
                     /translation="MDRKTVSAHLRKQFFPLLIAGGFTRSGDILRRELDGPVVHVVEV
                     QHRPRSGVFQVNLGAHLAALGGVAGGTPVPAEQMREYDCAWRSSVVSGFRNSSDSEFA
                     YGSTEDEASESVAFLVSEWERQSSEFFSPLSRFPESFHERASSSVDVGLHPAHLLTWA
                     RVADILGDAALRTRIVEAALPTVPERATSLRAQLLELR"
     gene            complement(7135..7218)
                     /locus_tag="VN041_12455"
     tRNA            complement(7135..7218)
                     /locus_tag="VN041_12455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7182..7184),aa:Leu,seq:cag)
     gene            7403..8083
                     /locus_tag="VN041_12460"
     CDS             7403..8083
                     /locus_tag="VN041_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HWU29884.1"
                     /translation="MGLLDSFEKGLERAVNSAFAKTFRSGIQPVEIASALRREADTKA
                     AVVSRERIITPSDYLVRLSPEDYERMLSLGHALNDELFALLTKHARTQGYSFSGPLHI
                     SIEGDAQVATGTVRVNSSSVESSVSWQGVVEVDGREHTLTSARTVIGRGSDAGITIAD
                     AGSSRRHAEILWDGERAMLRDLGSTNGTKVDGQKIREAALAPGSTFTIGRTQLTFRVV
                     PVSKGAAR"
     gene            8080..8568
                     /locus_tag="VN041_12465"
     CDS             8080..8568
                     /locus_tag="VN041_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWU29885.1"
                     /translation="MSDLLLLLLRIGFLVMLWFFVFAVVYSLRADLFGVRVRKMPAAE
                     APVPAAPVHQAPRPSAPAAKAATRLIITSGPKEGLEVPLGTADSMSIGRSSESGLVIR
                     DDYTSSHHARLTRRGDIWTLQDLGSTNGTFLDGHRLSDKPVQVQIGSAIKVGATTFEL
                     RA"
     gene            8584..9906
                     /locus_tag="VN041_12470"
     CDS             8584..9906
                     /locus_tag="VN041_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HWU29886.1"
                     /translation="MVFEGSSAAISHTGKVRSNNQDSGYSGANLFVVADGMGGHAGGD
                     VASSIAIGRMEGLDHPYASTDDAQASLQAAATTAASDLVRASKERPELAGLGTTLSAI
                     VMVDDHAVIGHIGDSRIYLYRDDALTQITTDHTFVQRLVDSGRITLEEARYHPRRSVL
                     MRVLSDMDIAPDLDMFVMPTHPGDRWLLCSDGLSGVVDEAHIAKAMAQGLAPGRTADN
                     LLKQALDGGAPDNVTVVLVDVGGAHPLSSGTPTVVGAASNPSSLPVAPPRSSLTGWLH
                     PVLQAANEPSHFEPDSDYFEEIIEEDKRRRTRRRVAWLAGLLVAVLALIGGGILAYNW
                     TQTRYFVGADDDSVVIYRGIQQDLGPIKLSTEVKDTFILLADLPSYQRDSVERTINAR
                     SLADAIAITERLRQSALPPIPVPIPTPTPSAPEPTPTPSDTQTEVPTT"
     gene            9903..11285
                     /locus_tag="VN041_12475"
     CDS             9903..11285
                     /locus_tag="VN041_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWU29887.1"
                     /translation="MTDVAADTSVLKALKRIRMPQKQRNREFWLLLFAIAISGASLTL
                     VQLGALGAIDPMILVIGGALAVLVFAVHVVLRVVASDADPFVLPIATTLTGLGIAMIY
                     RIDIAKQLSGWPAFSNKQLAWTAISLAGVIALVIALRNYRVLYRYTYIFGLAGIVLLI
                     LPFIPGLKARGATADVWVSLGGIFSFQPGELAKICLAIFFAGYLVRTRESLSSVGNRF
                     LGMTWPRMRELGPVLVVWIISLLIIVSQRDLGTGVLIFGMFIAMLYVATGKTSWVLIG
                     LAGVGIGAFLATRVLAYVQRRFTNWLDAFNPDIIQARGGSYQLVEGIFGLAHGGLIGT
                     GWGQGRPQITPLANSDYIIPSLGEELGIVGLFAILCLYMVFVSRGLRIGLAGQDDFGK
                     LLSTGLSFTIALQVFIMVGGVTRVIPLTGLTTPFLAAGGSSLVANWLIVALLLRISDG
                     VRRQPRVVIG"
     gene            11282..12736
                     /locus_tag="VN041_12480"
     CDS             11282..12736
                     /locus_tag="VN041_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWU29888.1"
                     /translation="MTKELRRLSFVILAMFLSLFLATSWIQVMDADNLAQNPANTRTR
                     LDSYQIQRGSILVGDSAIASSAPTDDKYQYQRVYTDAAMWAPVTGYFNPVLQSSTGIE
                     SALNAELSGTTSNSLVAEIERILSGQPQQGLSVKLTMNANAQRAAWDALQGLQGAVVA
                     IEPSTGRILAMASTPGFDTNKLAVHDANAVNTEHDALVADPTKPLYNRAIAGNLNPPG
                     STFKLVVASAALNSGKYTLDSTLPNPARYTLPGTSTEISNASGTTCGEGETTTIAQAI
                     IRSCNIPMAELAAELGDKAIRETAEKYGFNSEFAIPLKATPSKYPEGLNDPQTALTGF
                     GQGQVTATPLQMAMVAAGIANGGAVMKPRMVDAVIRDDFSVYKQYTDEAFDQAMPTDQ
                     AKALTSVMVQGVSSGAATGARIDGVDVAGKTGTAQNGSNPYTLWFTGFAPAENPKVAV
                     AVVVEDGGGRGQSGSGNGIAAPIAKKVIEAVLGR"
     gene            12733..14466
                     /locus_tag="VN041_12485"
     CDS             12733..14466
                     /locus_tag="VN041_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWU29889.1"
                     /translation="MRPTQGVSFGGRYELQSRIAIGGMGEVWEATDHVIGRTVAIKIL
                     KDEYMGDPGFLERFRAEARHAALVNHEGIASVFDYGEENGSAFLVMELVPGEALSTIL
                     ERDGALSADKTLDIVAQTASALQAAHAAGLVHRDIKPGNLLITPDGRVKITDFGIARI
                     ADQVPLTATGQVMGTVQYLSPEQASGHAASPATDVYSLGIVAYECLAGKRPFTGESQV
                     AIAMAQINEQPPPLPSTVPQPVQNLVMAMIAKKPADRPSSAATVSRAAQALRRGDLNS
                     AAIAVPAIAGIGVANADDATRILTGMGDGQATSILPTTAQLPTGQADAEEQTQKKKRS
                     PWTWPLVALIALLVIALGGTLLGLLLNQGDDKPVKTSSSAPVTSPSNTPTKDAAEPVN
                     IESLNLIGMTCDAAKTVLAAKDLTLDCVAGNPAGSEAEQGQIYELVDSGNVTPPATIR
                     VKYYADQVAIPAPSSAPTFSGTFTEGEEATVNWNAYVCPSGTPALSSYEVTITNAKFK
                     DGKNVSNFTPGTGSAVIVVDQGTAGKTVTATYRAFCGDRASGMSNSGTSTGIQPASPL
                     PTEGITPPPAN"
     gene            14524..16221
                     /gene="pknB"
                     /locus_tag="VN041_12490"
     CDS             14524..16221
                     /gene="pknB"
                     /locus_tag="VN041_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HWU29890.1"
                     /translation="MTTEPRILADRYRVDEVIGKGGMAQVHRGYDLTLGREVAIKILD
                     PELARDTAFRTRFRLEAQAASRMSHPSIVRVFDAGDPGAAPGANGETEPYIVMELVHG
                     TLLKDVIARGPLSVEDAIRYTDGILEALDYSHRAGVVHRDIKPGNVMITDKGTVKVMD
                     FGIARAVSDSSSTVAETTQIIGTAAYFSPEQAKGESVDSRTDLYSTGVVLYEMLTARQ
                     PFRGDSPVAVAYQHVSETPVPPSEVNEELPRALDAVVLRALAKDPYQRFPDAGSFRMA
                     LQGITTGHAPTKKEIGALTSELYGSSPRQAQETARSLRQLSTDTTMTRTQSGPPVAWI
                     WAGVALLAVLLVSVLFWVVSLSMRPPETPSSSVTVPNLVDLTVAKAKTELKDVGLVAD
                     VRHESSADFEVDHVIRTQPGTNAKVSKGESIIVYVSTGADTIDMPVIEGLSQDDATKV
                     LKDNGLVLGTIEQTNDPTAKAGTVLHSSQEAGTKVAPGTTVNLVVANGKVTVEDMTGF
                     TMDAATKKLEALSLTVTPTEVGACTDGQSPNTVLSMTVSPGEVPILSTVGLSYCTAG"
     gene            16424..17248
                     /locus_tag="VN041_12495"
     CDS             16424..17248
                     /locus_tag="VN041_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29891.1"
                     /translation="MASLTSRIRAAIAVSITLVLISSSSTAAIADASVPETDPVGFAA
                     FDTSSFSDVYRSMMENNDPAKVAALLQRFAESKSADREALKLGSLSDAEATDVLDEAS
                     VYAKAGEPLPPQLSARVGSGPNAPIQGEAINAGFSWEMNGRAGWSECEWFMCEVKSWI
                     DFRVVTDPNDVNTASYWTATEGGEGRLHGFEMDSMVYQDGNFTAGTYGKGWSTPGYGT
                     QWTFHPLDSNDGHTFQAEYNLNVSTPSGGMIYTSWSTGVSHTCGEPTPGAFRCLFD"
     gene            17245..17691
                     /locus_tag="VN041_12500"
     CDS             17245..17691
                     /locus_tag="VN041_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29892.1"
                     /translation="MSTDEMILRAQIAEGSQNDLWRLAGTRGLPEWAYQAILERGDEI
                     VLSSLAMNYECPPSILATIAAQGGEIATFARRNPNASPETKDPSPLGAQSARSVDLYL
                     TARNATQEQRVRFAALHRISPVDDGPSVGEAWLQINQEMAGQDGTE"
     gene            complement(17743..18339)
                     /locus_tag="VN041_12505"
     CDS             complement(17743..18339)
                     /locus_tag="VN041_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWU29893.1"
                     /translation="MSVRVLVVDNHDSFVHTLIGYLRELGAVVTLVEADALDENDFDP
                     RLDAHDAVMISPGPGTPADAGASLAVVRAAAERRMPLLGVCLGHQAIGEAFGGTVARA
                     PELMHGMVSQVTHDGSALFHGIGSPFAAGRYHSLALVESDLPAELRVTARAENGTIMA
                     VAHESLPILGVQFHPESVLTEHGHRLLGNWLRSVPGRA"
     gene            complement(18336..18491)
                     /locus_tag="VN041_12510"
     CDS             complement(18336..18491)
                     /locus_tag="VN041_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29894.1"
                     /translation="MYAALWRLLPGPWWVRLIILLALLAAVLYALFFWVFPWISPLIT
                     PGEVDVE"
     gene            complement(18504..19286)
                     /locus_tag="VN041_12515"
     CDS             complement(18504..19286)
                     /locus_tag="VN041_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HWU29895.1"
                     /translation="MHASAPTRRTRRAAGRSRATVTSVLGELLLTAGVIVLLFVAWQM
                     WIGDVIINAQKNAEGAATIRQWAEAPAPEPPPLVEAADGTTSYALPVLRHPADGQRFA
                     IMRIPRFGADYAKDIAGGTSRARTLDRIGIGLYTQSKMPGEVGNFSLAGHRTTWGKPF
                     NQLDKLKLNDAIVVETPEGWYTYRFRTLEYVKPTEVDVLDDVPQMPEQQAGERYITLT
                     ACSPLYSLAERIVAYGVFEGFQPRAEGPPASLAPVEPATPSL"
     gene            19324..19566
                     /locus_tag="VN041_12520"
     CDS             19324..19566
                     /locus_tag="VN041_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HWU29896.1"
                     /translation="MARSRVPEEPVDRPEGVDDAAPNAVWFKPVMVGFMLLGLAWILV
                     YYISGSLYPIPGLGGWNLGIGLAIALVGFLMTTRWR"
     gene            complement(19930..>20593)
                     /locus_tag="VN041_12525"
     CDS             complement(19930..>20593)
                     /locus_tag="VN041_12525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWU29897.1"
                     /translation="VAMAGRGGRPVVTYVLLAITTAIGLLQLLPGVGAQITSTLLFNA
                     VYLYPDLSGFPFEPWRLLTSVFVHGGILHLGLNMLALWMLGQTLEPMLGRGRYLALYL
                     ISGLGGSVAVALMAPGTSTVGASGALFGMMAALLIIGRHIGANVTGILVVLAINFAYG
                     FIVSGIAWQAHLGGLIVGALIALIYINTKRRDQRTWQIVLLSVLTVLVLGLAVAGNWI
                     LA"
BASE COUNT         3363 a         7136 c         6915 g         3179 t
ORIGIN      
        1 ctacgggtgc gccgttcgcc ctcgccgggg ccgcgcgcgt cacctcgacg ccgcactacg
       61 acgacgtgtc gcagctgatc ggccggctcg cctcggagca caccgtgaac gggctgcacg
      121 tgcacgtgga ggtgaccgga gacgaggagc gcgtccgtgc tctgctgcgc ctgcgcgagg
      181 cgcttccgct gctgctcgcc ctgagcgcga actcgccctt cgcgttcggc gtgccggcgg
      241 gactggccag ctggcggagc actctgatcc ggcggctccc ggtgtcgtgg ggaccgccga
      301 tcttcacgga tgccgaccac taccgccgca cggtcgaccg gctcgtcggg atcgggctgc
      361 tccccgcgcg ttcgtccgtg tcctgggccg tgcgcctctc ggaacagtac gacacggtcg
      421 agacccgggt cgccgatgcc cagctggagg tcgaggacac cctgctgttg gctgccctga
      481 cccgggcgat cgtcctcgcc gacgacgtca tccccaccag ctcggctccg gatgatctgg
      541 atgcttcgct gtggctcgcc gcgcggcacg ggacgcaggc ccggttctgc accgcggacg
      601 gcgggtcgga gtcggcatgg gcggccatgg agcgggtgct ggccggcatc cgccccgtgc
      661 tcgacgagct cggggacgcc gagttcgtcg atgatcgcct ggcctacctg cgggcggagg
      721 gcacgggcgc cgagcggcag ctgcgagcgc acgcgagcgg gggcgtcgaa gcgctcgcga
      781 gactcttcgg tgcaccgagt caaccgttgg cgtagcccat gcccatatgg tcgcgcacct
      841 cggcgagcgt cgcctccgcg atcgcgttcg cacgcacgtt gccctcgcgg agcacccggt
      901 cgacgaggcc aggatcggcg gcgagctcgg cacggcgggc tctatgcccg gagaggaatg
      961 cattcacggc atccgtggtg aacgccttca gcgcgccgct gccggcatcc ccgagccgct
     1021 cggcgacggc ctccggagtg ctgccggtgc acagtgcggc cgtggagagc agccccgaca
     1081 ccccgggccg gccctccggg tcgtaggtga tgacccggtc gctgtcggtg accgtgcgcc
     1141 gcacggcatc cgctgtctcg tcctcgctca tcgacagcgc gatcgcgttg ccgtaggact
     1201 tcgacatctt gcggccgtcg agcccgggga tctccggcgt ctcagtgacc atcgcctcgg
     1261 gttcggggaa cacctcgccg taccgctcgt tgaagcgccg cgcgatggtg cgggtgatct
     1321 cgacgtgcgg caggttgtcc ttgccgaccg gcacgaggtt gcccttgcag aacaggatgt
     1381 cggcggcctg gtgcaccggg tacgtcagca gcagcccgct cagggcgcgc ccggatgccg
     1441 cgtgctcggc cttcacggtg gggttccggt gcagctccgc ctcgctgacc aggctgagga
     1501 acggcagcag gagctggttg agcgccggca ccgcggaatg cgtgaagatc gtggtgcgct
     1561 cggggtcgag gcccgcggcc aggtagtcga gcaccgcgcc ccgcacgtgg tcggcgacgt
     1621 tcgccgcggt gtcgcggtcg gtgatcacct ggtagtcggc caggatgatg aacgtctcga
     1681 cgcccagcgc ctgcagacgc acgcgctcac ggatcgtgcc gaagtgatgg ccgagatgca
     1741 agctcccggt gggccgctcg cccgtgagaa cgcggaacga cccggggtcg tcgataatcc
     1801 tgcgttcgag ttccggcatc cgctcctgcg tcgccgcgta tgaatccgtc atggtgagtc
     1861 cttccgagag aagggctgca cgacgaggga tgccgcgaac cggggcgcgg gctgccgtgc
     1921 agcccgcgaa catgcgtcaa ccggctgcta ctgcagccac cacgaggaac gggtcaggga
     1981 acgcatgtgc atcaggatac gcctgtcggt cggggcagcc acacaatgtg agcaatgcta
     2041 acattgatcg catgcagacc atcctggggg cgaacgggca gatcgccgtc gagctcgccc
     2101 gcgacctgaa ccggcggggg agcgcgctgc gcctcgtcag ccgcaacccg cgcgaggtca
     2161 acgccggcga cgaactgacc cgcgcagacc tcctcgacgc cgcgcagacc gctgccgccg
     2221 tcaaggggag cgaggtcgtc tacttcaccg ccggccttcc ggcgaacgcc gagctgtggg
     2281 agcggcagtt tcccacgatg ctgaggaacg cgctcgatgc ctcgcgtgcc gccggcgcgg
     2341 cgttcgccta cttcgacaac acgtacatgt acccgcagga cgaccgcgtg cagaccgagg
     2401 aaaccccgtt cgcaccgctc ggccgcaagg gcagagtgcg agcggcgatg gccgagatgg
     2461 tgctcgacga gatggcgcgg ggcgacatcc ccgtgctgat cgcccgggcg ccggagttct
     2521 acggccccgg tcgcacgcag agcttcacca actcgctggt gctcgacccg ctgctggcgg
     2581 gccggaaggc gcgggtcccg gtgcgcgacg accgcgcgag gacgctcatc tggactccgg
     2641 atgccagtcg cggactcgcc gagctgggaa cgacgccgga tgccttcggc cagacctggc
     2701 atcttcccgt gcaccccgac cggctcacct accgcgaact catcgccctg gctggggagg
     2761 tcttcggcat cgagccccag tactccgtcg tcccgaagtg gatgctgagc gccgcaggcg
     2821 tcttctccgc ggaggcgcgc gtgatccgcg agctcctgcc gcggtatcag tacgacaacc
     2881 tcttcgactc caccaagttc cgcacgcgct tcccggcgtt cgcgatcacg gatcatcgtg
     2941 agggcctcgc cgtcatccgc gacgagaggc gcgccagcga tggacgatga gtcgacgccg
     3001 gaatccaggc gatccggcta ccaccacggc gatctgcgcg cgtccctgct gagcacggcg
     3061 atggagatgc tggaggcggg ggagagcttc tcgctgcgcg ccgtcgcccg cgaggccggg
     3121 gtctcgccca ccgcgcctta ccgtcacttc gccgatcgcg aggcgctgga gtcggcactg
     3181 gcggtgcagg gcttcgacga cctccggcga cgcctgtcgg ccgacgatgc gcgcgcgtcg
     3241 agcgtcgagg agctcgtcgg gttcgccgtg acctatgtcg cattcgccct ggagcgaccc
     3301 gcgctgttct cgctgatgtt cggccgggag tgcgatgaca gcgacgacga gcgcgtgcgc
     3361 gcggccgggg atctccatgc gctgctcgcc gattcgctgg gcgacgtgtt ccccgatcgc
     3421 gaccccggcc cgctcgccac ggccctgtgg tccctcacgc acgggctcgc cttcctgcat
     3481 ctggacggca agctctccgc cgcatccgcc gatgaagtcg ccgcccgcgt gcgcacctcg
     3541 ttcgccgcac tcggattcag ctgaaggaga aggtcatgac cgctcgcatc ctccacgtcg
     3601 tcaccaacgt cggccattac gacgacccgg cgcatccgac cggactgtgg ctctccgagc
     3661 tcgcccatgc gtgggaggtg ttcgagaagc agggcttccc gcagacgatc gtcagccctg
     3721 ccgggggtgc gtcgccgctg gagcccaggt cgctgcggtt ccccaactac gaccgcatcg
     3781 cgagggcctg gcatgaggac cccgcgcgca tggcgctgct gaagaacacg tcgagcccgg
     3841 atgccgtgga tgccgccgac ttcgatgcga tctacttcac cggcgggcac gccgtgatgt
     3901 acgacttccc cggcagcgag ggtctgcggc ggatcacccg agagatcttc gagcggggcg
     3961 gcgtggtctc gagcgtgtgc cacggctact gcgggctgct ggacaccacg ctgtcggacg
     4021 gatcccacct gatcgccggg cggcgcatga ccggcttctc ctggcgcgag gaggagctgg
     4081 cgaaggtcga cgagctcgtg ccgtacaacg ccgaggaggc cgctcgcgaa cgaggcgcgc
     4141 tctacgagaa ggcgctgatc ccgttcgtgt cgaacgtcgt ggtcgacggc cggctggtca
     4201 cgggccagaa ccccgggtcg gcgaaggcca cggcccggaa ggtcgtggag gtcctgggcg
     4261 gctgatcctt cgggcgaggg ctcagccagc gcgtccgtgc tgcgaggcgt gcggcccctg
     4321 cctcccgatt cttcaccgga ggcaggggcc atgcccttcc atcacttcgc gaggaacgcc
     4381 ttcagcgccg cgttgacgtc gtccgcgtgg gtccagagca ggccgtgcgg cgcgccctcg
     4441 atctcgacgt agtcggccgc agggacggcc tcgtggaagc gccgtgcggt cgcgtcgatg
     4501 gggaggatgt tgtccttcgt cccgtgcagg atgagggtcg gcttgcccgc gagccgaacg
     4561 gcctccacat caccgcggaa gtcctcgatc cacgccggca cgacggcgta tgcggcggcg
     4621 ggcgcgctgg tgaccgagag gttccagttc gccgtcacga cctgctggct gatccggctg
     4681 ccgaggttct cgtccaggtt gtagaagtcg ttgtagaact gggtgaacca cgagtagcgg
     4741 tcggttcggg cggcctccac gatgccgtcg aagacctcct gcggcacgcc ctccgggttg
     4801 tcgtccctct cgacgaggaa cggctcgaga gacgccagga aggcgagctt cgcgacgcgg
     4861 tcatgtccgt aggtggcgac gtaccgcgcg agctcgccgg ttcccatcga gaagccgacc
     4921 aggaccacgt cgcggaggtc gagcgtctcc agcacggtgt tgaggtccgc ggcgaaggtg
     4981 tcgtagtcgt acccgacgct caccttcgac gagttgccga atccgcgacg gtcgtaggtg
     5041 atgacacggt atccctgggc gagcagctcg cgggtctgca gctcccagct gtgaccgttc
     5101 aggggatagc cgtggatgag cacgaccggc tgcccttcgc cctggtcctc gtagtagagc
     5161 tcgatgggcg tggtgttctc ggtgccgacg ttgatgtacc ccatgggatc gttcctttcg
     5221 acctgagcga gaacggtcgt tctcgcttcg ctgatcacca tacgagaacg atcgttctca
     5281 tgtcaagtat gctgatgtca tgaccgagac ggatgcacgc gagcagatcc tcacagccgc
     5341 ggaggacctc tactaccgca agggatacgc ggccgtcggc atggacgagc tgcgcacggc
     5401 cgccggcgtg tcactcaggc ggctgtacgc cctcttcccc tcgaagatcg acgtcatcgc
     5461 ggccgtgctg cagcgcaagc acgacgagtg ggaggcagcc ctggaggcgg cggtgaccgc
     5521 cgccggagac agcccccggg atcggctgct ggccgtatac gcgcatctcg agaggtggtt
     5581 ctgcgacgac gacttccgcg gttgcgcctt catcaacgcg ttcggcgaac tcgggggcaa
     5641 cgatcccgag atcgcccgcg tcgtgcgaga gcacaaggcg gcctttcagg agcggatggc
     5701 cgtactggcg gatgaagcag gggtgccacg cgaggtcgca ttccagctct cgatactcgc
     5761 cgaaggggcg cagaccacgg cggcgatcgc cgctgcgccc caagcagcac ggcacgcgcg
     5821 cgcggcggcg gagatcctca tcgacgcgtc actcgcgccg catccgtccc gcgcgtgacg
     5881 acacgcgcgt ccggagccgc cgcaggccga gggcgaccag ccggagcacg ccggcgacga
     5941 tcgctccggc gagcgcaccc agcgagttcc agacgacgtc ccccagatcg ggcacgcgac
     6001 cggggatgcg cgactgcagc gtctccaccg cgatcgagag gacgaagccg atcatcggcg
     6061 ccagcaccca gagccgccgg ctgagcagga tcgccagcgc agctccgagc gggacgaaca
     6121 tgagcgcgtt cagggtgctc tccacctgcg cataggtcat cggctcgacc accgggttcg
     6181 cgacgaggtg cgcgaaccgc atgaacagtc cgcgcgccgg ccccacgagc gctctcggtg
     6241 cgagcgtgag cgtgaggatc gtcgcgaccg acgcgagcat cacgaagacc cggatgccgg
     6301 tgccggagcg ggcgggaagc tgcagactcg aagtcgtcat gacaccactc catccgatcg
     6361 gatgttgcgt gagcgtatgc tttccggccg aggcggttca tgatccgctc gtcgcattcg
     6421 cgcccgcgga atcagtgaga ggaccccgcc atggaccgca agaccgtctc ggctcacctt
     6481 cggaagcagt tcttcccgct gctgatcgcc ggtgggttca cccgcagtgg cgacatcctg
     6541 cggcgggagc tggacgggcc ggtcgtgcac gtggtcgagg tgcagcaccg gcctcgaagc
     6601 ggcgtcttcc aggtgaacct cggcgcccac ctcgccgcgc tcgggggcgt cgcgggaggg
     6661 acgcctgtcc ccgccgagca gatgcgggag tacgactgcg catggcgctc ctccgtcgtc
     6721 tcgggcttcc ggaacagctc cgactcggag ttcgcgtacg gttcgacaga ggatgaggca
     6781 tccgaatcgg tcgccttcct ggtctcggag tgggagcgtc agtcctcgga gttcttctcg
     6841 ccgctgtccc ggttccccga gagcttccac gagcgcgcat cgtccagcgt cgacgtcggc
     6901 ttgcatcccg cccatctgct cacctgggcg cgggtcgctg acatcctcgg cgacgcagcc
     6961 cttcgcacgc gcatcgtcga ggccgccctg ccgaccgttc ccgagcgcgc gacgtcgctg
     7021 cgcgcgcagc ttctcgagct gaggtgatgc ccgagttcca cggtgccgag cggccgatgc
     7081 tgggacgaaa cggaagagac ccgggtcctc tggacccggg tctcttccgt actgtgcgcg
     7141 aggggggact tgaaccccca cgtccttgcg aacactggca cctgaagcca gcgcgtctac
     7201 ctattccgcc actcgcgcag tgtctcgttc cgaagaactc aacctgctaa cactaacatg
     7261 tccgagcccg tggtccgaac acggcggccg gccacagcat ccgtcgaacg gcgtccgcaa
     7321 gccgtaatcc cgcctcaaga ggcgcatata cagcggggct ggctacgatg gccccaaaca
     7381 ggtcggatgc cagaggagcc ccgtgggact acttgacagc tttgagaagg gtctcgagcg
     7441 cgccgtgaac agcgcgttcg cgaagacctt ccgcagcggc atccagcccg tggagatcgc
     7501 ctcggccctg cgccgcgagg cggacaccaa ggcggctgtg gtcagccgcg agcgcatcat
     7561 cacgcccagt gactacctcg tgcggctgag ccccgaggac tacgagcgga tgctgagcct
     7621 cggccacgct ctgaacgacg agctcttcgc gctgctgacc aagcacgccc gcactcaggg
     7681 ctacagcttc tccggccccc tgcacatctc gatcgagggc gacgcgcagg tcgccaccgg
     7741 caccgtgcgt gtgaactcct cctccgtgga gagcagcgtc tcctggcagg gtgtcgtcga
     7801 ggtggacggc cgcgagcaca cgctgaccag cgcccgcacc gtgatcggcc gcggcagcga
     7861 cgcgggcatc accatcgcgg atgccggctc cagccggcgc cacgccgaga tcctctggga
     7921 cggcgagcgc gccatgctgc gcgacctcgg ctccaccaac ggcaccaagg tcgacgggca
     7981 gaagatccgc gaggcggcgc tcgcccccgg atccaccttc accatcggac gcacgcagct
     8041 caccttccgc gtggtcccgg tctcgaaggg agccgcccga tgagcgatct gctgctcctg
     8101 ctgctgcgca tcggcttcct cgtgatgctg tggttcttcg tgttcgccgt ggtctactcg
     8161 ctgcgcgccg acctcttcgg cgtccgcgtg cgcaagatgc cggccgccga agcccctgtc
     8221 cccgcagccc ccgtgcatca ggctccccga ccgagtgccc ccgcggcgaa ggccgcgacg
     8281 cgcctgatca tcacctccgg gccgaaggag ggccttgagg tccccctcgg caccgccgac
     8341 tccatgtcga tcggccgctc cagcgagtcc ggcctggtca tccgcgacga ctacacctcc
     8401 agccaccacg cgcggctcac ccgccgcggc gacatctgga ccctgcagga cctcggatcc
     8461 accaacggca cgttcctcga cggccaccgg ctgagcgaca agcccgtcca ggtgcagatc
     8521 ggcagcgcca tcaaggtggg cgccacgacc ttcgagctgc gagcgtaagg gcgcagcctc
     8581 cagatggtct tcgagggctc gagcgccgcg atctcccaca ccgggaaggt ccgctccaac
     8641 aaccaggact ccgggtactc cggggcgaac ctgttcgtgg tcgccgacgg catgggcggg
     8701 catgcgggcg gcgacgtcgc ctcgagcatc gcgatcggcc gcatggaggg cctcgaccac
     8761 ccctacgcgt ccaccgatga cgcgcaggcc tcgctgcagg cggccgccac gaccgcggca
     8821 tccgacctgg tccgcgcctc gaaggagcga cccgagctcg cgggcctcgg caccacgctc
     8881 agcgcgatcg tgatggtcga tgaccacgcg gtgatcggcc acatcggcga ctcgcgcatc
     8941 tacctgtacc gcgacgacgc gctcacccag atcaccaccg accacacctt cgtgcagcgg
     9001 ctggtcgact ccggccgcat caccctcgaa gaggcccgct accacccgcg ccgctcggtg
     9061 ctgatgcgcg tgctcagcga catggacatc gcgcccgacc tggacatgtt cgtcatgccc
     9121 acgcatccag gcgatcgctg gctgctctgc tccgacggcc tgtccggcgt ggtcgacgag
     9181 gcgcacatcg cgaaggcgat ggcccagggc ctcgccccgg gccgcacggc cgacaacctg
     9241 ctcaagcagg ccctcgacgg cggtgcgccc gacaacgtga cggtcgtgct ggtcgacgtg
     9301 ggcggggcgc atccgctctc ctccggcacc cccacggtcg tcggcgcggc atccaacccg
     9361 tcgagcctcc cggtcgcgcc gccgcgcagc tcgctgaccg gctggctgca ccccgtgctg
     9421 caggccgcga acgagccgag ccacttcgag cccgactccg actacttcga ggagatcatc
     9481 gaagaggaca agcgccgccg cacccgacgg cgcgtcgcct ggctggccgg cctgctcgtc
     9541 gcggtgctcg cgctgatcgg cggcggcatc ctcgcctaca actggacgca gacgcgctac
     9601 ttcgtcggcg ccgacgacga cagcgtggtg atctaccgcg gcatccagca ggatctcggg
     9661 ccgatcaagc tgtccaccga ggtgaaggac accttcatcc tgctggccga cctgccctcg
     9721 taccagcgcg actccgtcga gcgcaccatc aacgcccgct ccctggcgga tgccatcgcc
     9781 atcaccgagc gactgcggca gagcgcgctc ccgcccattc ccgtgccgat cccgacgccg
     9841 acgccctctg cgcccgagcc caccccgacg ccttccgaca ctcagacgga ggtgccgacg
     9901 acatgaccga cgtcgccgcc gacaccagcg tcctgaaggc gctgaagcgg atccggatgc
     9961 cgcagaagca gcgcaaccgg gagttctggc tgctgctgtt cgccatcgcc atctcgggag
    10021 catccctgac gctcgtgcag ctcggcgcgc tgggcgcgat cgaccccatg atcctcgtga
    10081 tcggcggggc gctcgctgtg ctggtcttcg ccgtgcacgt ggtgctgcgc gtcgtggcat
    10141 ccgatgccga tccgttcgtg ctgccgatcg cgacgaccct caccggtctc ggcatcgcga
    10201 tgatctaccg catcgacatc gccaagcagc tctccggatg gccggcgttc tcgaacaagc
    10261 agctcgcctg gaccgccatc tccctggcgg gcgtgatcgc gctggtcatc gccctgcgca
    10321 actaccgcgt gctctaccgg tacacgtaca tcttcggcct cgccggaatc gtgctgctga
    10381 tcctgccgtt catccccggt ctgaaggcga ggggcgcgac cgccgacgtg tgggtgtcgc
    10441 tgggcggcat cttctcgttc caaccgggtg agctggccaa gatctgcctg gcgatcttct
    10501 tcgccgggta tctggtgcgc acccgcgaga gcctcagctc ggtgggcaac aggttcctcg
    10561 gcatgacctg gccgcggatg cgcgagctcg gtccagtcct ggtcgtctgg atcatctcgc
    10621 tgctgatcat cgtcagccag cgcgacctcg gcaccggcgt gctgatcttc ggcatgttca
    10681 tcgcgatgct gtacgtcgcg accggtaaga ccagctgggt gctgatcggc ctggcgggcg
    10741 tcggcatcgg cgcgttcctc gccacccgcg tgctcgcgta cgtgcagcgc cggttcacga
    10801 actggctgga cgcgttcaac cccgacatca tccaggcacg cggcggcagc taccagctcg
    10861 tcgagggcat cttcggtctg gcgcacggcg gcctgatcgg caccggctgg ggtcagggcc
    10921 gtccgcagat caccccgctc gccaacagcg actacatcat cccgagcctc ggcgaggagc
    10981 tcggcatcgt cggcctgttc gcgatcctct gcctgtacat ggtgttcgtc agccgcggtc
    11041 tgcgcatcgg gctcgccggc caggacgact tcggcaagct gctgtccacc ggcctgtcgt
    11101 tcacgatcgc cctgcaggtg ttcatcatgg tcggcggtgt gacgcgcgtc atcccgctca
    11161 ccggcctgac cacgccgttc ctcgccgccg gcggatcgtc gctggtcgcc aactggctca
    11221 tcgtcgcgct gctgctgcgc atcagcgacg gcgtgcgccg ccagccccgg gtggtgatcg
    11281 gatgaccaag gagctcaggc gcctcagctt cgtgatcctc gcgatgttcc tgtcgctgtt
    11341 cctggccacc agctggatcc aggtgatgga tgccgacaac ctggcgcaga acccggccaa
    11401 cacgcgcacc cggctggaca gctaccagat ccagcgcgga tcgatcctgg tcggtgactc
    11461 ggcgatcgcc agctcggcgc cgacggacga caagtaccag taccagcggg tctacacgga
    11521 tgccgcgatg tgggcgcccg tgacgggcta cttcaacccg gtgctgcagt cctccacggg
    11581 catcgagagc gcgctgaacg ccgagctctc cggcacgacc tcgaactcgc tggtcgccga
    11641 gatcgagcgc atcctctccg gtcagccgca acagggtctg agcgtgaagc tcacgatgaa
    11701 cgccaacgcg cagcgcgcgg cgtgggacgc cctgcagggc ctgcaggggg cggtcgtcgc
    11761 gatcgagccg tccaccgggc gcatcctggc catggcatcc acccccgggt tcgacacgaa
    11821 caagctggcc gtgcacgatg cgaacgccgt gaacaccgag cacgacgcgc tggtcgccga
    11881 cccgaccaag ccgctgtaca accgtgcgat cgccggcaac ctcaacccgc cggggtccac
    11941 gttcaagctc gtcgtcgcga gcgcagcgct gaactcgggc aagtacacgc tcgactccac
    12001 gctgccgaac ccggcccgct acacgctgcc cggcacctcg accgagatct ccaacgcctc
    12061 gggcaccacc tgcggcgagg gcgagacgac gacgatcgcc caggcgatca tccgcagctg
    12121 caacatcccg atggcggagc tggcggccga gctgggcgac aaggcgatcc gcgagacggc
    12181 ggagaagtac ggcttcaact ccgagttcgc gatcccgctg aaggccaccc cctcgaagta
    12241 ccccgagggg ctgaacgacc cgcagaccgc gctgaccgga ttcggtcagg gacaggtcac
    12301 cgcgacgccc ctgcagatgg ccatggtcgc cgccgggatc gccaacggcg gcgccgtgat
    12361 gaagccgcgg atggtggatg cggtgatccg cgacgacttc tcggtgtaca agcagtacac
    12421 cgacgaggcg ttcgatcagg cgatgcccac cgaccaggcc aaggcgctca cctctgtgat
    12481 ggtgcaggga gtcagttccg gggcggcgac gggtgcaaga atagacggcg tcgacgtcgc
    12541 cggaaagacg ggcacggcgc agaacggatc caacccgtac acgctgtggt tcacaggttt
    12601 cgcaccggcg gagaatccga aggttgcggt ggcagtcgtc gtcgaagacg gcggtggaag
    12661 aggacagtcg ggttccggca acggtattgc cgccccgatt gcgaagaaag tcatagaggc
    12721 ggtgctgggc agatgaggcc gacgcagggt gtgtcgttcg gtggtcgcta cgagctgcag
    12781 tcgcggatcg cgatcggtgg catgggtgag gtctgggagg cgacggatca cgtcatcgga
    12841 cgcaccgtcg cgatcaagat cctcaaggac gagtacatgg gcgacccggg cttcctggag
    12901 cgcttccgcg ccgaggcccg ccacgccgcg ctcgtcaacc atgagggcat cgcgagcgtg
    12961 ttcgactacg gcgaggagaa cggctccgcg ttcctcgtca tggagctcgt gcccggtgag
    13021 gcgctgtcca ccatcctcga gcgcgacgga gcactctccg ccgacaagac gctcgacatc
    13081 gtcgcgcaga cggcatccgc cctgcaggcg gcgcacgccg ccggcctggt gcaccgcgac
    13141 atcaagccgg gaaacctgct gatcacgccc gacggccgcg tgaagatcac cgacttcggc
    13201 atcgcgcgca tcgccgacca ggtgccgctg accgccacgg gccaggtgat gggcaccgtg
    13261 cagtacctgt cgcccgagca ggcctccggc cacgccgcat cgccggccac tgacgtgtac
    13321 tcgctgggca tcgtcgcgta cgagtgcctg gcgggcaagc gcccgttcac gggtgagtcg
    13381 caggtcgcca tcgcgatggc gcagatcaac gagcagccgc cgccgctgcc gtccaccgtg
    13441 ccgcagccgg tgcagaacct ggtcatggcg atgatcgcca agaagcccgc cgaccggccg
    13501 tcctcggccg cgacggtgtc gcgcgccgcg caggcgttgc gccgcggaga cctgaactcg
    13561 gccgcgatcg ccgtgcccgc catcgccggc atcggcgtgg cgaacgccga cgacgcgacg
    13621 cgcatcctca ccgggatggg cgacggtcag gccaccagca tcctgccgac cacggctcag
    13681 ctgcccacgg ggcaggcgga tgccgaggag cagacgcaga agaagaagcg cagcccgtgg
    13741 acgtggcccc tggtggcgct gatcgcgctg ctcgtgatcg cgctcggcgg cacgctgctg
    13801 ggtctgctcc tgaaccaggg cgacgacaag ccggtcaaga cctcgagctc ggcgccggtc
    13861 acgtccccgt cgaacacacc gaccaaggac gccgccgagc cggtgaacat cgagtcgctg
    13921 aacctgatcg gcatgacctg cgacgcggcg aagaccgtgc tcgcggcgaa ggatctcacg
    13981 ctggactgcg tcgccgggaa ccctgccggg tccgaggccg agcaggggca gatctacgag
    14041 ctggtggaca gcggcaacgt cacccctccg gccacgatcc gggtgaagta ctacgccgac
    14101 caggtcgcga tccccgcacc ctcctccgcg cccaccttct ccgggacgtt caccgagggc
    14161 gaggaggcga ccgtcaactg gaacgcctac gtctgcccct ccggaactcc tgcgctctcc
    14221 tcgtacgagg tcacgatcac gaacgcgaag ttcaaggacg gcaagaacgt ctcgaacttc
    14281 acgccgggca ccggctccgc ggtgatcgtc gtcgaccagg gcaccgcagg caagaccgtg
    14341 acggccacct accgggcctt ctgcggcgat cgcgcctcgg gcatgtccaa ctcgggcacg
    14401 tccaccggca tccagccggc atcgcccctg ccgaccgagg ggatcacgcc gcctcccgcc
    14461 aattgatcag gttgcagcgg gtaatctgac aggagacttc aaggcatcag ggggagacgg
    14521 gacgtgacga cagagccgcg cattctcgcc gaccgctatc gggtcgatga ggtgatcggc
    14581 aagggcggaa tggcccaggt gcaccgcggg tacgacctga ccctgggccg agaggtcgcg
    14641 atcaagatcc tcgaccccga gctcgcgcgt gacacggcgt tccgcacccg attccgcctg
    14701 gaggcgcagg ccgcctcgcg catgtcgcat ccgtcgatcg tccgcgtctt cgacgccggc
    14761 gacccgggcg ctgcccccgg tgcgaacggc gagaccgagc cctacatcgt catggagctg
    14821 gtgcacggca cgttgctgaa ggacgtcatc gcccgcgggc cgctgtcggt cgaggacgcg
    14881 atccgctaca ccgacggcat cctcgaggcg ctggactact cgcaccgcgc cggcgtcgtg
    14941 caccgcgaca tcaagcccgg caacgtgatg atcaccgaca agggcaccgt caaggtgatg
    15001 gacttcggca tcgcccgcgc agtgtcggac tcgtcgtcga ccgtcgccga gacgacgcag
    15061 atcatcggta ccgccgccta cttctcgccc gagcaggcca agggcgagtc ggtcgactcg
    15121 cgcaccgatc tgtactccac gggcgtggtg ctgtacgaga tgctcaccgc gcgtcagccg
    15181 ttccgcggcg actccccggt ggccgtcgcg taccagcacg tcagcgagac gccggttccg
    15241 ccgtcggagg tgaacgagga gcttccccgt gcgctggatg ccgtggtgct gcgcgctctc
    15301 gccaaggacc cgtatcagcg cttccccgac gccggctcgt tccgcatggc cctgcagggc
    15361 atcacgacgg ggcatgcgcc cacgaagaag gagatcggcg ccctcaccag cgagctgtac
    15421 gggtcgagcc cgcgtcaggc gcaggagacg gcccgatcgc tgcggcagct gagcaccgac
    15481 acgaccatga cccgcaccca gtccgggcct ccggtggcgt ggatctgggc cggcgtggcg
    15541 ctgctcgccg tactgctggt gtcggtgctg ttctgggtcg tcagcctctc gatgcgcccg
    15601 ccggagacgc cctcatcgtc ggtgaccgtg ccgaacctgg tggacctgac cgtcgccaag
    15661 gcgaagaccg agctcaagga cgtcgggctg gtcgccgacg tgcgccacga gtccagtgcg
    15721 gacttcgagg tggatcacgt gatccgcacg caacccggca cgaatgcgaa ggtctccaag
    15781 ggcgagtcga tcatcgtgta cgtgtccacc ggcgccgaca ccatcgacat gcccgtgatc
    15841 gaggggctgt cgcaggacga cgcgaccaag gtgctgaagg acaacgggct cgtgctcggc
    15901 acgatcgagc agaccaacga tccgaccgcc aaggcgggca ccgtgctgca ctccagccag
    15961 gaggccggca ccaaggtcgc gccggggacg acggtgaacc tggtggtcgc gaacggcaag
    16021 gtgacggtcg aggacatgac cggcttcacg atggatgccg cgaccaagaa gctcgaggcg
    16081 ctgtcgttga cggtcacccc gacagaggtc ggcgcctgca ccgacggcca gtccccgaac
    16141 accgtgctgt cgatgacggt ctcgcccggc gaggtgccga tcctctcgac ggtcggcctg
    16201 agctactgca cggccggctg atcccgctcc gcggctgagg gccttttcgc gtacgcggcg
    16261 gctgatcctc agccgcggcg atcagtcccc ccgagtcctc acaaacgtgt gctcccgtag
    16321 tcaccgaacg ttcgagcttg gcgtgtcgtc cttttggggg acagacagac aaacgcatct
    16381 catgatttgc tgtagccaac caactcaaac gattggattg cacatggcca gcctcacctc
    16441 acggatcaga gccgccattg cggtctcgat cacgttggta ttgatttctt cctcttccac
    16501 tgcggcgatc gccgacgctt cggtgcccga gaccgatcct gtgggtttcg cggcgttcga
    16561 cacttccagc ttctctgatg tgtaccgatc aatgatggag aacaacgacc cggccaaagt
    16621 tgcggcgctg ctccaacgat ttgctgagtc gaagagcgct gaccgcgagg cgttgaagct
    16681 cggcagcctc tccgatgccg aagcgactga tgttcttgac gaagcatccg tttacgcaaa
    16741 ggctggggag ccactccccc ctcaactgag cgcgcgagta gggagcggac ccaatgcgcc
    16801 gatccaggga gaagcgatta acgccggatt ctcctgggag atgaacggtc gtgcggggtg
    16861 gtcggaatgc gaatggttca tgtgtgaggt gaaatcttgg atcgatttca gggtcgtcac
    16921 cgatcccaac gatgtcaata cggcctccta ctggacagcc acggaggggg gagagggccg
    16981 gctacacggg tttgaaatgg actcgatggt ctatcaagat ggcaatttca ctgccggcac
    17041 ctacggcaaa ggctggagca ctcccgggta cggtacgcag tggactttcc acccgttgga
    17101 ctctaatgac gggcacacgt tccaggccga atacaatctc aatgtatcca caccaagcgg
    17161 tggcatgatc tacaccagtt ggagcacagg cgtatctcac acctgcggcg agcccactcc
    17221 cggggccttc cgttgtctgt tcgattgagc accgacgaaa tgatccttcg agcgcagatt
    17281 gcggagggat cgcagaacga tctttggcga ctggccggta cacgcggcct gccggagtgg
    17341 gcgtatcaag ccatcctcga gcgcggagac gagatcgtgc tctccagcct cgcgatgaac
    17401 tacgagtgcc cgccaagcat cctcgcaacg attgcggctc aaggcggcga gatcgccacc
    17461 ttcgcacgcc ggaacccgaa tgcgagtcca gagacgaagg acccctcacc gctaggggct
    17521 cagagcgcgc gttccgtcga cctctacttg acagcgcgta acgccacaca ggagcagcga
    17581 gttaggttcg ccgcactcca cagaatcagt ccggtcgatg acggcccctc cgtcggcgaa
    17641 gcctggctgc agattaatca ggaaatggcg ggccaggacg gtaccgagta ggcgggcctt
    17701 cgtcaggaag ggccgactca ccgccccacg gccgtagatc gctcatgccc ttcccggcac
    17761 gctcctcagc cagttgccga gcaggcggtg cccgtgctca gtgagcacgc tctcggggtg
    17821 gaactgcacg cccaggatcg gcagactctc gtgggcgacg gccatgatcg tgccgttctc
    17881 ggcgcgcgcg gtgacccgca gctcggcggg aagatccgac tcgaccaggg cgagggagtg
    17941 gtaccgcccg gcggcgaacg gagagccgat gccgtggaac agcgcggatc cgtcgtgggt
    18001 gacctgcgac accatgccgt gcatgagctc gggcgctctc gcgaccgtgc cgccgaacgc
    18061 ctccccgatg gcctgatggc ccaggcatac gcccagcagg ggcatccgcc tctccgccgc
    18121 cgcgcgcacg acggccagcg acgccccggc gtcggcgggg gtgccggggc cgggggagat
    18181 catgacggcg tcgtgggcgt ccagccgggg atcgaagtcg ttctcgtcca gggcatccgc
    18241 ctccaccaaa gtcacgacgg cgccgagttc gcgcaggtac ccgatgaggg tgtgcacgaa
    18301 gctgtcgtgg ttgtcgacca cgagcacccg cacactcatt ccacgtcgac ctctcccgga
    18361 gtgatcagcg ggctgatcca ggggaacacc cagaagaaca gcgcgtagag caccgccgcc
    18421 agcagggcga gcaggatgat caggcgcacc caccaggggc cgggcaggag gcgccacagt
    18481 gctgcgtaca tcggtggttc tcctcagagg gatggggtgg cgggttcgac gggggcgagg
    18541 gacgccggcg ggccctcggc gcgcggctgg aagccctcga aaacgccgta ggcgacgatg
    18601 cgctcggcga gggagtacag cggtgagcac gcggtgaggg tgatgtagcg ctcgcccgcc
    18661 tgctgctcgg gcatctgcgg cacatcgtcg agcacgtcga cctcggtggg cttgacgtac
    18721 tccagcgtgc ggaagcggta ggtgtaccag ccctcgggcg tctccacgac gatggcgtcg
    18781 ttcagcttga gcttgtcgag ctggttgaag ggcttgcccc aggtggtgcg gtggccggcg
    18841 agcgagaagt tgccgacctc gccgggcatc ttcgactgcg tgtacaggcc gatgccgatc
    18901 cggtcgagcg tgcgtgcacg gctggtgcca ccggcgatgt ccttggcgta gtcggcaccg
    18961 aaccggggga tgcgcatgat cgcgaagcgc tggccgtcgg ccggatgccg caggacgggc
    19021 agcgcatagc tggtggtgcc gtccgcggcc tcgaccagcg gcggcggctc gggagcgggg
    19081 gcttcggccc actggcggat ggtcgccgcg ccctcggcgt tcttctgggc gttgataatg
    19141 acgtcgccga tccacatctg ccaggccacg aagagaagca cgatcacgcc ggccgtgagc
    19201 agcagttcac cgagcacgct cgtgaccgtc gcgcgggagc gacccgccgc gcgccgggta
    19261 cggcgtgtcg gcgcggaagc gtgcatgagg ggaagtctat tcggcccggc tagactgatc
    19321 cccatggcac gatctcgcgt acccgaagag cccgtcgacc gccccgaggg cgtcgacgac
    19381 gccgccccca acgccgtctg gttcaagccc gtgatggtcg gcttcatgct gctcgggctc
    19441 gcctggatcc tggtgtacta catctccggc tcgctgtacc cgatccccgg cctcggcggc
    19501 tggaacctcg gcatcggcct ggccatcgcc ctggtcggct tcctcatgac cacccgctgg
    19561 cgctgacgcc agtcgcacac agactcaaag ggcccctcgc gcagcgaggg gccctttctc
    19621 gtcttcaccc accatcaact cctgccggac ggcgcccgca tccacctgag ggagcgggga
    19681 ggttcccgga gcgagcatcg gggagggccc cgcgaagcgg gaggggtcgc ccgttctgcg
    19741 agcgcaggga acctccccgc tcccggcaac ccatgacatc tctgcgcccg ccctcagcat
    19801 ccggcccgcc ccagcatccg cccacagctt tccacaggag ttctccccag agttatccac
    19861 aggctgggga gaattacacc ggtgttattc acacaggggg ataaccctgt taacaacagt
    19921 tgatcagcct taagcgagaa tccagttgcc tgcgacggcg agtccgagca ccaggactgt
    19981 gagtaccgag agcaggacga tctgccaggt gcgctggtcg cggcgcttgg tgttgatgta
    20041 gatcagcgcg atgagggcgc cgacgatgag tccgccgagg tgggcctgcc aggcgatgcc
    20101 ggacacgatg aagccgtagg cgaagttgat ggccagcacc acgaggatgc cggtgacgtt
    20161 ggcgccgatg tgtcgcccga tgatcagcag ggcggccatc atgccgaaca gcgcaccgga
    20221 ggcgccgacc gtcgaggtgc ccggtgccat gagcgcgacg gcgaccgagc cgcccaggcc
    20281 gctgatgaga tacagcgcga ggtaccggcc tcggcccagc atcggctcga gggtctgccc
    20341 cagcatccag agcgcgagca tgttcagtcc gaggtgcagg atgccaccgt gcacgaagac
    20401 cgaggtgagc agccgccacg gctcgaacgg gaagccggag aggtcgggat agaggtagac
    20461 ggcgttgaac agcagcgtgc tggtgatctg cgccccgacg cccggcagca gctgcagcag
    20521 gccgatggcc gtggtgatcg ccagcagcac gtaggtgacg acgggccggc cgccgcggcc
    20581 ggccatcgcg acc
//
LOCUS       DATMSX010000400        27538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_157974,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000400 DATMSX010000000
VERSION     DATMSX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 27538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27538
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157974"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VN041_12530"
     CDS             complement(<1..197)
                     /locus_tag="VN041_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HWU29898.1"
                     /translation="MRTDADATDPHRKSGSAPRVIHTGQALVDLIVEVPDLPARGQNV
                     MASSANRYAGGAVTVLLAAARL"
     gene            567..1157
                     /locus_tag="VN041_12535"
     CDS             567..1157
                     /locus_tag="VN041_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29899.1"
                     /translation="MSIPNPPAGAPAPQSPPPVPPVGVQPPAPKKTNVVAMVAMIVAI
                     VGFIFACIPGALIVGWILLPIAFILSIVSLFLKGDRKWMGIVGLIVSVVGTIVGFIVF
                     FAVVATSFNDAFGGGETTVSQPVEKPADDAKPADDKKPAEAAQGTRENPYPLSSQISN
                     DDWTVVVNSVNTDGNPVVADANQFNDQAPPARTTRS"
     gene            1154..1411
                     /locus_tag="VN041_12540"
     CDS             1154..1411
                     /locus_tag="VN041_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29900.1"
                     /translation="MNYTVTYNGGKKGLAAEVQVAMVTSAGNVVNSYDNLVMLKDGFG
                     LDEMYKGASATGSVAFAVPDGDAAVVRVTPGLLADEVFVKP"
     gene            complement(1466..1855)
                     /locus_tag="VN041_12545"
     CDS             complement(1466..1855)
                     /locus_tag="VN041_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HWU29901.1"
                     /translation="MADTQAGWYDDGSGTMRWWDGQQWTDQVQPPAPHEPGLDGFINR
                     ARADAEAGAQPRPAPQGMSYVVLQVVLKEKFFGSGSGNLVELEAVINRQAALGYRLHT
                     ITTASSGSTGFGGGDRIQATMVFEKLV"
     gene            1997..4114
                     /locus_tag="VN041_12550"
     CDS             1997..4114
                     /locus_tag="VN041_12550"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HWU29902.1"
                     /translation="MQTDLATPQLIFNMPQHLTVPIYQRPYVWTEDEQWAPLWADIRR
                     ITEHRLTAPTSTVSHFLGAVVTQDAAAGPVGVQEYEIVDGQQRLTTLQLVLDAAAAAF
                     EERGLTSLAGQLAFLTHNSTMFIGGAEPGLKLRHTNRDRVAFDEVMLAAPPIDYRRLD
                     HGDSLIARAHEFFATHIGAWLDDDREQSSARAEALAMSLQLSLQLVIIRLTATEDSQE
                     IFETLNARGTPLTAADLIKNYVFQRLRLEGTNAADAYARWPFETKFWEAEVSVGRFPT
                     TRSALFLSQWLVSQVGREISPRSTFSRFKFFTEHETERTMSELLDIITAQAKHYEELT
                     RRAADPHSDLDRVALHFYRMSVAQVEITKAIALWLTSPETKYRPQTIASVIDRVESWI
                     MRRRLLRLQSGDLGRIASELISYARNAADDEVIAKVEQYLTGLVSTSTYWPGDDEVRS
                     SLQHVHFYQRFSQPMQRVFLQAVEDWHRGYTAEGQSKTGIRVHRDKQQVEHLLPRTWQ
                     THWPVADAAAGAERDDNVHRLGNLTLLTGPLNASVSNAAWLGDGGKRAELRKHDVLLM
                     NRVVIDGSADGWDEVRIAERTDAMITALQETWPVPEGHEGKTVDRSARFSKATTSYSD
                     LISAGLIEVGAVLVCTEERWHDARGTLLAGGQMLYDGKTYSSPAAAANAVRGGRSGNA
                     WYFWRVENGAWLKDLREELLRQQ"
     gene            complement(4333..5559)
                     /locus_tag="VN041_12555"
     CDS             complement(4333..5559)
                     /locus_tag="VN041_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HWU29903.1"
                     /translation="MNTSAPLNVTNEITCPHCGTAFTVDEAQYADIVQQVRTVEFEAE
                     LHARLAEAEKVKQTEIALAVANATRQEHEAAAAKDVEIERLSATIKAAVTDQELAVAK
                     AVSTVEKELSSVKSRLELQTAEQKVKDATLKESHAKELAVKDDLIARYKDMKAKLSVK
                     LLGETLEQHCEIEFNRMRSLAFPNAEFAKDNDASAGTKGDYVFRDYSDDGIEYVSIMF
                     EMKNESDISATKKKNVDFLAKLDKDRNDKGCEYAVLVSMLEEDSDLYSGITDVSHLYP
                     KTFVIRPQFFLAIIALLRNAAQDTITVKSELEQVKKQNIDITDFESELESFKSAFGRN
                     YDLAKRKFDGAIKEIDAAIDRLQKVKDALLGSENNLRLANDKANALTVKKLTKGNPTM
                     QAKFAEIESFDETDAA"
     gene            5757..7739
                     /locus_tag="VN041_12560"
     CDS             5757..7739
                     /locus_tag="VN041_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29904.1"
                     /translation="MSTTSPLLIDLAGAARLAGVRRPVVSMWRSRFATAVDPFPSQIT
                     EDAGRALFDASQVAEWLVRTGHGSNSDARADAASEAVPPGFAFTDEDAVAELEAVIAL
                     YAQVGTLEALSDAELRQAAKAADPQDLMFRSEVEAHAERGAPWLDYAELLIDAAYSPS
                     AALALVHRRASSVRGTAASTGPLVAEAVSLVVESALALVGGDAVSLILDGSDAKLSVA
                     LADALGDTATLTLPRAEVARRVRRRLLTDGHWVTGADDSPPTPRSVVVARVPSQRSAD
                     SVPTLLAVDEVSLGLRDDDAAVVIGPARILTDPLGPAETRVRADILRSGRVRGIARLA
                     TGLVETASREALALWMLGAPHGDVAIADRVTVVADLTGMPLTPATRADLVSDVIASMG
                     GLRDVRAHAFRFSRPARTPSLAARGGSLVASAGIRPKASPARSDIPALIDTAADAVRS
                     DITPIPLSTTAHATPVAASVEDLIQDGHLKVVRGVRLDSNMLGTDGLIVVTASDLDAP
                     ETIGDSRVDQLAFATQHPRASLTRPGDVVFRTSPRAAAWVDADGSKVVVSPARVLRIT
                     AGDPGGLVPEVVAADIAVAPAGPGAWKRWMLRRVAPHTTAPLRKALAGIAAARADLEQ
                     RAARLNDYADLIVAGATSGAVTMIEENDAADAAATQ"
     gene            7748..9379
                     /locus_tag="VN041_12565"
     CDS             7748..9379
                     /locus_tag="VN041_12565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HWU29905.1"
                     /translation="MPRTPKTAPQAPSTMKELKDTLWKAADKLRGSMDASQYKDVILG
                     LVFLKYVSDAFDERRVGIRAELTAGGYDEEQIADLIDDTDEYTGRGVFWVPTNARWSF
                     LAENAKGLGPSMDGPEKSIGLLIDEAMDAVMQANPQLLGTLPRIFNRDNVDQRRLGEL
                     IDLFNSARFAAQGQGGSDGRRARDLLGEVYEYFLEKFARAEGKRGGEFFTPPSVVRVL
                     VELLQPTHGRVYDPACGSGGMFVQAEKFIDAHHGEGSDISVYGQELNERTWRLAKMNL
                     AIHGLNGNLAPRWGDTFARDLHPDLQADFVMSNPPFNIKDWARNEDDPRWRFGVPPAG
                     NANYGWIQHILSKLAPGGQAGVVMANGSMSSNSGGEGQIRAEIVESDLLSCMVALPTQ
                     LFRSTGIPVCVWFFAKDKGARAGEVLFIDARNLGHMIDRAERALSEEDIAKIAGTFHS
                     WIGHNAVVARAERDETPHPYADIPGFCYSATLAEIKAADYALTPGRYIGAPEAEDDGE
                     PIEEKLARLRAGLEAQFAESARLADVVREQLGRIS"
     gene            9376..10557
                     /locus_tag="VN041_12570"
     CDS             9376..10557
                     /locus_tag="VN041_12570"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWU29906.1"
                     /translation="MRLDAVCSHVVDCEHKTAPIDESGEYFAVGTPAMRDHRIEYAEA
                     RRISRDTFEAWTRRLRPQPGDLLLAREAPVGPIVRLPESAHVAPGQRTVLLRPDAAAT
                     SSTYLYYLLRSPRMQAELLLKAEGSTVHHLNVADIRALEVDLPPLAEQQAIAEVLGAL
                     DDKIAANTALAESLTRLSALECRALAASTTRSVPLADLVTTQYGVTTSAVVEPQGTGL
                     VRVTDINKRPWIEWDSVPGCSLDKGVREKYLLNPGDIVVARMADPGKAAWISLGDPPA
                     VFASYLVRLQATIEYHAPFIYEFLRGEQYRRYCEGAMQGSVQKNMNAKVIVGTTMPLP
                     DRAALREYAGRFHGLRALMSSALRENRTLAATRDALLPQLMSGKLRVRDAERIATEAG
                     V"
     gene            10557..11774
                     /locus_tag="VN041_12575"
     CDS             10557..11774
                     /locus_tag="VN041_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWU29907.1"
                     /translation="MAFAVVDFETTGILPSYHHRVVEIGLTHVEDDGTISGRWETLIN
                     PDRDLGPQRIHGIRAADVLDAPLFSDVAAEFAELLQGRVFAAHNASFDLRFLQAEFER
                     AGYWLDGGTPSVCTMRLGASFGLGASGSLVQSCSACAIDLTHAHSAGADSHAAAQLLA
                     HYQRSSADWPGWPAYWEQTADAGRRYRYPTGSRTGIVAKPRAEASATPPSFLERISTE
                     AVRETVDGAAAQYVALLDRCLLDGFLSSSERSQLAVVATELGLDRATVEGLHRDYFVT
                     LERRAWADGVLTEDEKTDLAAVGMLLELGDDAIAAAIDDLAPDGVDEDAQTAFTLNPG
                     DLVVLTGDMSRPRSDWEAELQARGFICHPAVTKKVSLVVAADPDSLSGKAKKARDYSI
                     PIVSEHWLKEFLQ"
     gene            11771..12391
                     /locus_tag="VN041_12580"
     CDS             11771..12391
                     /locus_tag="VN041_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29908.1"
                     /translation="MTTTTPRRTAPAWAKVLLVVVILALIGAAAFAGLTIGKFLGASE
                     ERDVQVVRSVQGEEQVILLTAGLTDIKEKHGKQTFFGLFDVPLSDRTSFLRYEFEAKF
                     GIEGKQVDVEPLGDKAYRITIPEFIFLGYDDPEFSVATESNGILSWTTPAIDTLKATE
                     ELLSDKAVAEHIDGFRPLLEEQAITFYTRIVSSIEPDAKLTFEFSE"
     gene            12425..13612
                     /locus_tag="VN041_12585"
     CDS             12425..13612
                     /locus_tag="VN041_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWU29909.1"
                     /translation="MTENAEEARTSEPGARDSIPKIDERPFLDATSEAKSRYGRFNLA
                     VIGSSGVGKSSLVNAVFGRDWAKVGKGLPVTRGVQYYHDDSLGIWDVEGFEIGSAVAP
                     REQLRAHLNVIAQHPADQQIAVVWYCVKSNDDRLTAADIEMIRELDAQGLPVILVLTK
                     VDVGGSVIPGKRTIPKGVQQFIDWLESPVDGEDQQLHIPYQRVIVTAVRDKNGKDSGY
                     GLGELVTETLALSPEDEKDAFRIAQRLNLPWKREMARTVIAGASTAAAGAAAVPVPVA
                     DAAILAPIQLGMMGRIATIYDLDIRTMLSAGTLAQVGVQLAGRALASSFLKLIPGAGS
                     VVNAGVAAALTGATGEGWMRLCEQVHTGKLDLAAINQSFGDYVPGFLDVIRKMAEQKL
                     SKS"
     gene            13659..16853
                     /locus_tag="VN041_12590"
     CDS             13659..16853
                     /locus_tag="VN041_12590"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HWU29910.1"
                     /translation="MTTYSESQWEQDALDMLAEPLGWRPLTGEQIAPGTDERDSWDDL
                     LVRPRLLDALRRLNPGIPGEYLQQALAEIASPKSQDAITENHRIHQYLVGGYPLSYLD
                     ADGVEQNPRLRLLGSDPSENDWIAVNQLTVRQGDLHRRFDVVLFVNGMPLVIIELKRA
                     GSASTGVAPAHAQLQTYLREFPMVFRFCVLTLASDGLEAKYGTPFTPLNHFAPWNVDD
                     DGAPLTQPRVVGGVSVEPIDDALGGLFNQERFLQLVRSFTAFDENADGLTKRIAKPHQ
                     YFAVTKAVGSTVQAVETNGKAGVVWHTQGSGKSMEMELYTNLVSRHPKLKNPTVIVVT
                     DRTELDGQLFQTFDQSLLLAEKPVQVRERAQLRDELSNRVTGGIYFTTLQKFGLTEAE
                     KQSGTQHPLLTDRRNVIVVVDEAHRSHYDNLDGYARHLRDALPSATLIAFTGTPISFA
                     ERDTRAVFGDYIDIYDLTRAVDDGATVPVYFEPRLIKVSFAEGSTEEDIDQAADEYTL
                     GLDDTERARLEASVAVVNAVYGAPERIDTLAADLVAHWEERRERMKPFLESPGKALIV
                     GGTREICARLYEAIVALRPDWHSDALDKGRIKVVYSGSASDQPPVSDHVRRDSENKAI
                     KARLKDATDELELVIVKDMMLTGFDAPPLHTLYLDRPLKGALLMQTLARVNRTFRGKQ
                     DGLLVAYAPLADNLAKALSEYTVSDQENRPIGRDVDEAVAIANELVAQIREMLAGCDW
                     RSIVKAGGARPWRTAATTATNFLRDPGNPLNAEELGPERLAARYRTATGQLSRAWALA
                     SGRDGLAELHEEIAFYEEVRVWMAKFDAAERQSRDEPIPEEISRLLGSLVADAIAPGD
                     VLDIYEAAGLPRIALDNLGPEFLAKAQAAPNAHLAIEALRASLTDASATATRGNLVRQ
                     RAFSERIAEIMNRYTNQQLTSAEIIAELVELAKEVAEEAARGANFSPPLSNDELAFYD
                     AVATNDSAIDIQGEDVLAQIARELVAVMRRDVRTDWTVRDDVRAKLRSSIKRLLVFYK
                     YPPDKQPEAIKLIMDQMESMAPRYADERRAPNHE"
     gene            complement(17738..18286)
                     /locus_tag="VN041_12595"
     CDS             complement(17738..18286)
                     /locus_tag="VN041_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29911.1"
                     /translation="MPRPRSEKARHAVLEAMLRALAADGYAAVTIEGLAAEAGVSKQT
                     IYRWWPSKAAILGEALLEGSLPGFELPMTSDLRADLRAVLSAQNPDTVAIARALIEVT
                     ATDAELGLKLNARLAAPIREWTAVRLAQAVDAGDVRPDVDAAVVADQLIAIGSYSALV
                     GGPTEAGKADGIVDVMLRGIGC"
     gene            18384..19379
                     /locus_tag="VN041_12600"
     CDS             18384..19379
                     /locus_tag="VN041_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU29912.1"
                     /translation="MALTAHSTIGEWMNDATGGPLIRGLFEKTGADPELLTPVLGLPL
                     QQLVAMSQGALPQSVVDDLVRAANGGEVPEDTVPTGWVENITPGRFTGKTVIVTGAAS
                     GIGRATASRITREGGRVIAADISADKLDALKDELPGIVTVAGDLTQQDAIDAVIAAAG
                     DQIDGLANVAGINDDFSPAGETTDAVWDRVIAINLTAPFKLMRAVIPVMEKNGRGAIL
                     NVSSEAGLRGNASGNAYTASKHGIIGVTKSAAVMYGPKGIRVNSVAPGGVATGIPMPP
                     HMSEYGSARLAPFQQAIPSVATAEHLAASITFLLSDDAVNINGAVLASDGGWSVQ"
     gene            complement(19437..19880)
                     /locus_tag="VN041_12605"
     CDS             complement(19437..19880)
                     /locus_tag="VN041_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29913.1"
                     /translation="MPEIDWSNDQLRTFLTALRSGDAFNRGVDVDQDERDRFLAQVRR
                     RIVPEIRARVLAEVGATLDPRGVAAIVLEVLEVLEDNTWGAERTWLMVTEDPWGYLTE
                     FVAAEIRKAYRKALHRTADDKTLKGIEQASNRRAIDAGGDAADAA"
     gene            20108..20302
                     /locus_tag="VN041_12610"
     CDS             20108..20302
                     /locus_tag="VN041_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29914.1"
                     /translation="MDHDRSMPAEAVAAVDGLLTSSGIVHDTRIRPDAAHGYTMADTS
                     SYDETAGDWHFTRLRESLPD"
     gene            complement(20271..21287)
                     /locus_tag="VN041_12615"
     CDS             complement(20271..21287)
                     /locus_tag="VN041_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29915.1"
                     /translation="MKGEAVAPAGILWDTRLMASDRRNPYVTATTPSRWPSFLIGVGA
                     ALVVMVPGWLTGGGLQVPTRWDAERLTGRRPFAFLPLDLDHVFPLFATLVLGGVLAGL
                     IVRMLRRRRPVQVWPAAWGVLLAQLAAAVQSFIALLGRLGIGGGSEQAVGAMLMDGTQ
                     LLYLLGLVLVVVVGLLFAQLGLLFATRRSPAFGALAVALAAPLVTNWIGGPLVVFVSI
                     DSYPEWLAIVLRWLPAVIVGLALVWCGVRPWGRLSVWVIGLAALWVMPAAITSISYAV
                     GIRAIQNDPQEIPRAIAQYFVPMLHAGVAPVVVALVVGVVGTVVRMLVRRSPVSREET
                     LEGV"
     gene            21450..23777
                     /gene="purL"
                     /locus_tag="VN041_12620"
     CDS             21450..23777
                     /gene="purL"
                     /locus_tag="VN041_12620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWU29916.1"
                     /translation="MSAEAPQTTHVPDSVENAIATPEKEQPYAALGLKDDEYARIKEI
                     LGRRPTSGELAMYSVMWSEHCSYKSSKNYLRRFGQKVSDEMKQRLMVGMGQNAGVVDV
                     GEGWAVTFKAESHNHPSFIEPFQGAATGIGGIVRDIISMGARPVAVMDALRFGAIDHP
                     DTPRVVHGVTAGISFYGNCLGLPNIGGETVFDAVYQANPLVNALAVGVLRHEDLKLAN
                     ATGVGNLVVLFGARTGGDGIGGASILASDSFDDGGPTKRPAVQVGDPFAEKVLIECCL
                     ELYKGELVEAIQDLGAAGISCATSELAANGNSGMRVSLDNVLLRDPTLTAEEILMSES
                     QERMMAIVAPEKLDAFMAVVHKWEVETSVLGEVTGDGRLVIDWQGERIVDVDPSTVAV
                     DGPVYDRPVAYPAWIDALQADAAELLPRSDDAATLREQFFKILGSPNLADTSWITNQY
                     DYYVGGNTALAFPDDAGMMRVDEESGLGFAIATDANGRYCQLDPYAGAQLALAEAYRN
                     VAVTGANPTAITDCLNFGSPENPEVMWQFGQTVDGLADGCYELRTPVTGGNVSFYNQT
                     GDVPIHPTPLVGVMGIIEDVSRRIPSGWQDEGQNIYLLGTTSTELSGSAWADVVHDHL
                     GGLPPKVDLAREKDLAGLISAARDEWLISSAHDLSEGGLGQALAEGVMRFGVGARVWL
                     TELMERDGVDAASALFSESTGRVIVTVPREDDVKFRGLCEGRGYPVLRIGVTDSEPAL
                     EVQDVFTATVAELREASKATLPAVFGPTVTEPVAV"
     gene            23786..23923
                     /locus_tag="VN041_12625"
     CDS             23786..23923
                     /locus_tag="VN041_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29917.1"
                     /translation="MNRTDEDYGDLGERRNRRIRLVAWVTIVALIVGGGGATALTLLL
                     G"
     gene            23931..24167
                     /locus_tag="VN041_12630"
     CDS             23931..24167
                     /locus_tag="VN041_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29918.1"
                     /translation="MTRRKRPYKSTAIVLWALGVMLTLPALLPLASGPPGAQAFAPIG
                     ISIGVLVLIAGTLLFFTGPKEPLEDPREAPVDDD"
     gene            24257..24577
                     /locus_tag="VN041_12635"
     CDS             24257..24577
                     /locus_tag="VN041_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU29919.1"
                     /translation="MTIQIADVAHDANLEGHLIGSPVSIIREYATTAGGGPRLHRHPY
                     AETFIIHRGSALFTVGEEQLVGVGGQILVVPALVSHRFEVLDGGYEATHVHASERFIT
                     EWLE"
     gene            24669..24851
                     /locus_tag="VN041_12640"
     CDS             24669..24851
                     /locus_tag="VN041_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29920.1"
                     /translation="MSDSTPNDEPLSDEPLNDDPLENGLDKGSGDPNDDRDFLMDQPP
                     AQVKPQEDRDDEDASR"
     gene            complement(24942..25997)
                     /gene="gnd"
                     /locus_tag="VN041_12645"
     CDS             complement(24942..25997)
                     /gene="gnd"
                     /locus_tag="VN041_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWU29921.1"
                     /translation="MQLGMIGLGRMGANIVRRLMKDGHECVAYDVNQDAVAALAAEGA
                     TGASDLKDFAAKLQTPRVVWMMVPASLTGKVVDQIAEVLEPGDILIDGGNSNYRDDVR
                     RAKALREKGIEYVDAGTSGGVFGLERGYCLMVGGSDAAVNHIGPLLKTIAPGVGEIER
                     TPGRTGELTPEEQGYLHCGPSGAGHFVKMVHNGIEYGIMASIAEGLNILKNADAGVRE
                     AEHSAEIAPLEEPEYYQFPIDTAKVTELWRRGSVISSWLLDLTATALNENPSLDGLAG
                     RVSDSGEGRWTVKAAVDVGVPVPVLAASLFERFASRDEDQFANQVLSAMRLQFGGHKE
                     LPAGDSLEAGGRKSEVG"
     gene            complement(26139..>27538)
                     /locus_tag="VN041_12650"
     CDS             complement(26139..>27538)
                     /locus_tag="VN041_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWU29922.1"
                     /translation="RPVVAGPLDVDAHHALAREAASRSIVLLKNEGGILPLAPSQKVA
                     VIGAFATEPRFQGAGSSLINPTRVDAALDALREVGGDAVSYAPGFAVKGGAIAASGRS
                     ADDLRDEAVAAASAAEVAVVFLGLPASQESEGFDREHIDLPAEQLAVLDAVVAANPNT
                     VVVLSNGGVVALPFADRVPAIVETWLLGQAGGGAVADVLYGAVNPSGKLTETVPVRLE
                     DNPSYGNFPGEFGHVRYGEGLLVGYRWYDAKDLQVTYPFGHGLSYTTFAYGDATASVR
                     DGDIVVTLEVTNTGDRDGREIVQAYTSLPGSLVQRAPRELKAFASVAVAAGETVAVEL
                     VIRREDLAYWDVRVDRFVVEGGEYVIEVGASSRDIRSSAAVGLEGDAVRLPLDMNSSI
                     GEVMAHPFAGPIVQQALAGLAEPQDAAGASMMEGDGMEKMMASFPIGRIVGFPGIPVT
                     REQIEQLIAGANAQG"
BASE COUNT         4725 a         9464 c         8852 g         4497 t
ORIGIN      
        1 aggcgcgcgg cggcgagcag caccgtcacc gcgccgcccg cgtatcggtt cgcggacgag
       61 gccatgacgt tctgtcctcg agcggggagg tccggcacct cgacgatgag gtcgacgagc
      121 gcctggccgg tgtggatcac ccggggtgcg gagccgctct tccggtgggg gtccgtggcg
      181 tcggcatccg tgcgcatgcg gacagcctac tgagcgagtg gcggtcggcc acggcatcct
      241 gtgctccgtc gatgtccgga cgcgtcgcgg accgttcatc ctgcgcattt cacgccctca
      301 accgcgccgg tgtgcgcgtt cgctctgcac gtcggcgcca gtcctcgggc ggctcggcac
      361 ccgaaaatct acgcggggag atcgttgtcc cccgcagtcg ctatagttcg actcagccgg
      421 tcctcgcgga ccacggagat ctcccccgac tccacgctcg acgcccactg ggagggcggt
      481 gatcggagtg cgcggggacc ggctccctgc gacgcccgtc gtggggcagg ccggtgctcc
      541 gcgcaccttc tcccaaggag atccgcatgt ccatccccaa cccgcccgcc ggtgcccccg
      601 cgccgcagtc tcccccgccc gtgcctccgg tcggagtgca gcctcccgca ccgaagaaga
      661 cgaacgtcgt ggccatggtg gcgatgatcg tcgcgatcgt cggcttcatc ttcgcgtgca
      721 tccccggcgc tctgatcgtc ggctggatcc tgctgccgat cgcgttcatc ctcagcatcg
      781 tgtcgctgtt cctgaagggc gaccgcaagt ggatgggcat cgtgggtctg atcgtctcgg
      841 tcgtcgggac gatcgtcggg ttcatcgtgt tcttcgccgt ggtcgccacc tcgttcaacg
      901 acgccttcgg cggcggcgag acgacggtgt ctcagccggt cgagaagccg gccgacgacg
      961 cgaagccggc ggatgacaag aagccggcgg aggccgcaca gggtacgcgt gagaatccgt
     1021 acccgctgtc gtcgcagatc agcaacgacg actggaccgt cgtggtcaac agcgtcaaca
     1081 ccgacggcaa ccccgtggtc gccgacgcca accagttcaa cgaccaggct ccgccggctc
     1141 gcactacgag atcgtgaact acacggtcac gtacaacggc ggcaagaagg gcctcgcagc
     1201 cgaggtgcag gtcgcgatgg tcaccagcgc gggcaacgtc gtgaacagtt acgacaacct
     1261 cgtgatgctg aaggacggct tcggcctgga cgagatgtac aagggcgcgt cagccaccgg
     1321 ttcggtggcg ttcgccgtgc ccgacggcga cgccgccgtc gtgcgggtga cgcccggtct
     1381 gctcgcggac gaggtcttcg tcaagccgta aggcggaggt gcacgaccgg tcaccgcccg
     1441 gtcgcgcacc ctgccgtgtt ggtcttcaga cgagcttctc gaacaccatc gtcgcctgga
     1501 tgcgatcgcc accgccgaaa ccggtgctcc cggacgacgc ggtggtgatc gtgtgcaggc
     1561 ggtagcccag cgcggcctgc cggttgatca cggcttccag ctcgacgagg ttgccggagc
     1621 cgctgccgaa gaatttctcc ttcagcacga cctgcagcac gacgtagctc atcccctgtg
     1681 gagcagggcg cggctgcgcg ccggcctcag catccgctcg ggcccgattg atgaacccgt
     1741 ctaggcctgg ctcgtgcggc gccggcggct gaacctggtc ggtccactgc tgcccgtccc
     1801 accaccgcat cgtgccggat ccgtcgtcgt accagccggc ctgtgtgtca gccatcagat
     1861 cccttctcgc cgacccggtt tcgggtcgaa tgattgccac cgtagcggcg ccgaccctct
     1921 ccgcagtgcg gttgatccgc cctcatagaa tgcgagtgac cgcgcacccc cgagcgccgt
     1981 ggcgaaggag taattcgtgc agaccgatct ggcgacgccg caactgatct tcaacatgcc
     2041 tcagcacctg acagtgccga tctaccagcg tccgtacgtg tggaccgagg acgagcaatg
     2101 ggctcctctt tgggccgaca tccgacgcat caccgagcac cggctcaccg ctcccacttc
     2161 gacggtgagt catttcctcg gcgcagtcgt cacgcaggac gcagctgcgg ggcccgtcgg
     2221 cgtgcaggag tacgagatcg tcgatggcca gcagcggctc acgacgctgc agttggtgct
     2281 ggatgccgcc gcagccgcgt tcgaggaacg agggctcacc agccttgccg ggcaactcgc
     2341 gtttctcacg cacaacagca cgatgttcat cggcggcgcc gaaccgggcc tgaaactgcg
     2401 ccacaccaat cgcgaccgtg tcgcgttcga cgaagtgatg ctcgccgcgc cgcccatcga
     2461 ctatcggagg ctcgaccacg gcgactcact catcgcccgg gcgcatgagt tcttcgccac
     2521 ccacatcggt gcatggctcg acgacgatcg cgagcagagt tcggcacgcg ccgaggcgct
     2581 cgcgatgtcg ctgcagctct cgctccagct ggtcatcatc cggctcactg cgacggagga
     2641 ctcccaggag atcttcgaga ctctgaacgc acgcggaact cctctcactg ccgctgatct
     2701 gatcaagaac tatgtcttcc agcggttgcg cctcgaaggc acgaacgctg cagacgcata
     2761 cgcccgctgg ccgttcgaga ccaagttctg ggaggcggag gtcagcgtcg gccgcttccc
     2821 gacgacgcgg agtgcgctct tcctcagtca gtggctggtg tcgcaggtcg gtcgagagat
     2881 cagcccacgg tcgacgttct cccgcttcaa gttcttcacc gagcacgaga ccgaacgcac
     2941 gatgtccgag ctgctcgaca tcatcaccgc acaggcgaag cactatgagg agctgaccag
     3001 gcgcgcagcc gaccctcact ccgatctcga ccgcgttgcg ctgcacttct atcggatgag
     3061 cgtcgctcag gtcgagatca ccaaggcgat cgccctgtgg ctcacgtctc cggagacgaa
     3121 gtaccgaccg cagacgatcg cgtccgtcat cgaccgcgtc gagagctgga tcatgcgacg
     3181 acgccttctg cgtctgcaga gcggtgacct cgggcgcatc gcatccgagc tgatctcgta
     3241 cgcccgtaac gctgccgacg acgaagtgat cgccaaggtg gagcagtacc tgaccggcct
     3301 ggtctcgaca agcacctatt ggcccgggga tgacgaggtt cgctcgtcgc ttcagcacgt
     3361 gcatttctat cagcggttct cccagccgat gcagcgggtc ttcctgcagg ccgtcgagga
     3421 ctggcaccgc gggtacaccg ccgaagggca gtcgaagacc ggcatccggg tgcatcgcga
     3481 caagcagcag gtggagcatc ttctgccgcg cacgtggcag acgcactggc cggtcgcgga
     3541 tgccgccgcg ggcgccgaac gagatgacaa cgtgcaccgt ctcggcaacc tcactctgct
     3601 gacggggcca ctcaacgcgt cggtttcgaa cgcggcgtgg ctgggcgacg gagggaagcg
     3661 cgcggagctc aggaagcacg acgtgctgct gatgaaccgc gtggtgatcg atggctctgc
     3721 cgacggctgg gacgaggtga ggatcgcgga gcggacggat gcgatgatca ccgctctgca
     3781 ggaaacatgg cctgtacccg aggggcacga gggcaagact gtcgaccggt ctgcgcgctt
     3841 cagcaaagcg accacctcct attccgacct gatcagcgcc gggctgatcg aggtcggtgc
     3901 cgttctggta tgcaccgaag aacgctggca cgacgcgcgc ggcactctgc tggccggcgg
     3961 gcagatgctc tacgacggca agacgtacag ttcaccggcc gcagctgcga atgccgtccg
     4021 gggcggacgc agcggcaacg cctggtactt ctggcgggtc gagaacggcg cctggctgaa
     4081 ggacctccgc gaggagctgc tacggcagca atgaggcccg ttcaggtctc gatattctga
     4141 cgctggaaac tcccgtcgcg gctgacgttt cgaaatcaca ggtggaccac aacgcgctcg
     4201 atctggccgc ctcggtgacg tgcacacggc gcccgttcac gcaagggtat gcaagaacgt
     4261 tgagcacgca agagcagcag ctaggcgtca tgcgatgccc cgctgcgtta cgcagcgggg
     4321 catcgctcca ccttaggcgg catcggtctc gtcgaacgat tcgatctcgg cgaacttcgc
     4381 ctgcatcgtg gggttgccct tagtcagctt cttcaccgtg agcgcattcg ccttgtcgtt
     4441 ggccaggcgc aggttgttct cggagccgag cagagcgtcc ttgaccttct gcagccgatc
     4501 gatcgccgcg tcgatctcct tgatcgcacc gtcgaacttg cgcttcgcga ggtcgtagtt
     4561 gcgcccgaac gcggacttga agctctcgag ctcggattcg aagtcagtga tgtcgatgtt
     4621 ctgcttcttg acctgctcga gttcgctctt gacggtgatc gtgtcctgcg cggcgttgcg
     4681 aagcagcgcg atgatcgcca ggaagaactg cggtcggatg acaaacgtct tcgggtacag
     4741 gtgcgagacg tcggtgatcc ccgagtagag gtcggagtcc tcctcgagca tcgacacgag
     4801 cacggcgtac tcgcagccct tgtcgttgcg gtccttgtcc agcttcgcga gaaagtcgac
     4861 gttcttcttc ttcgtcgccg agatgtcgga ctcgttcttc atctcgaaca tgatcgagac
     4921 atactcgatg ccgtcgtcac tgtaatcacg gaagacgtag tcgcccttcg tgccggccga
     4981 ggcatcgttg tccttggcga actcggcgtt tgggaaggcg agcgaacgca tgcgattgaa
     5041 ctcgatctcg cagtgctgct ccagggtctc gcccagcagc ttgaccgaca gcttggcctt
     5101 catgtccttg tagcgcgcga tcaggtcatc cttcacggcg agctccttgg cgtgcgactc
     5161 cttgagcgtc gcatccttga ccttctgctc agcagtctgg agctccagcc ggctcttcac
     5221 gctgctgagc tccttctcga cggtagagac tgccttcgcg acagcgagct cctggtcggt
     5281 gacagctgcc ttgatcgtgg cactcagccg ctcgatctca acgtccttcg cggcggcggc
     5341 ctcgtgctcc tgccgcgttg cgttggcgac ggcgagggcg atctcggtct gcttgacctt
     5401 ctccgcctct gccagccggg cgtggagttc ggcctcgaac tccacagtgc gaacctgctg
     5461 gacgatgtcg gcgtactgcg cctcgtcgac ggtgaaggca gtcccgcaat gcgggcacgt
     5521 gatctcgttg gtcacgttga gcggtgcgct ggtgttcatg gttacctccg gttgtgtcgt
     5581 gcgtggatgt caccggcgga gcgctccctc cccagaggcg ttccgcctgt gacggcgacg
     5641 tgtgtcgctg ggaacgatct tgggcgtctg gcatcgtgaa cggcattcag atagctgata
     5701 agggtagtta tcggtgattc tcaacttcgc gcggtgatcg tggatagatt ggccacatgt
     5761 ccacgacctc accccttctc atcgacctag ccggcgccgc ccggctggcc ggtgtgcgtc
     5821 gtccggtggt gtcgatgtgg cggtcccgtt tcgcgacggc cgtggatccg ttcccgtcgc
     5881 agatcaccga ggatgccggc cgggcactgt tcgacgcatc tcaagtcgcc gaatggctcg
     5941 tccgcactgg ccatggcagt aactccgatg cacgtgcgga cgcggcctcg gaagcagtgc
     6001 cgccaggctt cgccttcaca gacgaggatg cggtcgccga gctggaggcc gtcatcgctc
     6061 tttacgcaca ggtcggcacg ctcgaagcgc tgtcggacgc cgaactgcga caggcggcca
     6121 aggccgccga cccgcaagac ctgatgttcc gatccgaggt cgaagcgcac gcggaacgtg
     6181 gcgcgccatg gctcgactac gcagagctcc tgatcgacgc cgcctattcg ccgtccgccg
     6241 cactggcgct ggtccatcgg cgcgcatcat ccgttcgtgg aactgcagcg tcgacaggac
     6301 cactcgtcgc tgaagcggta tccctcgtcg tcgagtcggc gctcgccctg gtcggcggag
     6361 acgccgtctc actcatcctt gacggctcgg acgccaagct ctccgtggcg ctcgccgatg
     6421 cgctgggcga cacagcgacc ctcacccttc cgcgtgcgga ggtcgctcgc cgcgtgcgcc
     6481 gacgcctgct cacggacggc cactgggtga cgggagcgga tgacagcccg ccgacgccgc
     6541 gatccgtcgt cgtcgcgcga gtcccatcac agcgctccgc cgattcggtc cccaccctgc
     6601 tcgcggtgga cgaggtttcg ctcggcctgc gagatgacga cgcggcagtg gtgatcggtc
     6661 ctgctcggat cctgactgac ccgctcggcc ctgcagagac acgggtgcgt gccgatatct
     6721 tgcgctccgg tcgggtgcgc ggaatcgcac gcctggcgac aggacttgtc gagacagctt
     6781 cgagggaggc actggcactg tggatgctgg gcgcgccgca cggagatgtc gcaatcgcag
     6841 accgtgtcac cgtggtcgcc gatctgactg gcatgccgct cactcccgcg acccgcgcgg
     6901 atctcgtatc ggatgttatt gcgagcatgg gtggccttcg ggacgtgcgc gcgcacgcct
     6961 tccgcttctc acgccctgcg cggacgccgt ccctggcggc gcgcggcgga tcgctcgttg
     7021 cttcggccgg catccgtccc aaggcgagcc cggcgcgctc cgacattccg gcgcttatcg
     7081 ataccgctgc cgacgcagtg cgttcggaca tcacaccgat cccgttgagc acgaccgctc
     7141 acgcgactcc ggtggcggcg tcggtcgagg acctcatcca ggacggacac ctgaaggttg
     7201 tcaggggtgt ccggctggac tcgaacatgc tcggaacaga cgggctgatc gtcgtgacag
     7261 catcggacct cgacgcccca gaaacgatcg gggacagccg ggtcgaccag ctcgcgttcg
     7321 cgacgcagca tccgcgcgct tccctcaccc ggccgggcga cgtcgtcttc cggacgagcc
     7381 cgagggctgc ggcgtgggtc gacgccgacg gttcgaaggt cgtcgtgtcg cctgcgcgag
     7441 tgctgcgcat caccgcgggt gatccgggag gcctcgtacc cgaagtcgtc gctgccgaca
     7501 tcgccgtggc acctgctggg ccgggcgcgt ggaagcgatg gatgctgcgc cgcgtcgccc
     7561 cgcacaccac cgccccgcta cgtaaggcgc tggccggcat cgctgccgcc cgcgccgacc
     7621 tcgagcagcg agcagcgcgc ctgaacgact atgccgacct gatcgtcgcg ggagcgacgt
     7681 caggagctgt caccatgatc gaagagaacg acgccgccga cgcggcagcc acgcagtaag
     7741 gacacacatg cctcgcacac cgaagaccgc accgcaggcg ccgtcgacga tgaaagagct
     7801 gaaggacacg ctctggaaag ccgccgacaa gctgcgcgga tcgatggacg cctcccagta
     7861 caaggacgtc atcctcgggc tcgtgttcct caagtacgtg tcggatgcgt tcgatgagcg
     7921 ccgcgtcggg atccgtgccg aactgaccgc cggcggatat gacgaggagc agatcgccga
     7981 tctcatcgac gacaccgatg agtacaccgg gcgtggagtc ttctgggttc cgacgaacgc
     8041 acgctggtcg ttcctcgccg agaacgcgaa ggggctaggc ccgtcgatgg acggcccgga
     8101 gaagtcgatc ggcctgctga tcgatgaggc gatggatgcc gtcatgcagg cgaacccgca
     8161 actgctgggc acgctgccac gcatcttcaa tcgcgacaac gtcgaccagc gccgcctggg
     8221 cgagttgatc gacttgttca actcggcacg gttcgccgct caggggcagg gcggatcgga
     8281 cggacgccgt gcgcgcgacc tgttgggcga ggtgtacgag tacttcttgg aaaagttcgc
     8341 cagggcggag ggcaagcggg gcggggagtt cttcacgccg ccgagcgtgg tccgcgtgtt
     8401 ggtcgagcta ctgcaaccga cgcacgggcg agtgtacgac cccgcgtgcg ggtcgggcgg
     8461 catgttcgtg caggcggaga agttcatcga cgcacatcac ggtgagggca gtgatatctc
     8521 ggtgtatggg caggagctca acgagcgtac ctggcgtctc gcgaagatga acctcgcgat
     8581 ccatgggttg aacggcaacc tcgcgccgcg ctggggcgat acgttcgcgc gtgacctgca
     8641 ccccgatctt caggcggact tcgtgatgtc gaacccgccg ttcaacatca aggactgggc
     8701 gcgcaacgaa gacgacccgc gctggcggtt cggcgtgccg ccggcgggga acgcgaacta
     8761 cggctggatc cagcacatcc tgtcgaagct cgcacctggc gggcaggcgg gcgtcgtgat
     8821 ggcgaacggg tcgatgtcgt cgaactccgg tggcgagggg cagatccgtg ccgagatcgt
     8881 cgagtccgac ttgctgtcgt gcatggtggc gctgccgacg cagctgttcc gctcgacggg
     8941 gattccggtg tgcgtgtggt tcttcgcgaa ggacaaaggc gcacgggccg gcgaggtgct
     9001 cttcatcgac gcccgcaacc tcggccacat gatcgaccgc gccgaacgcg cgctgagcga
     9061 ggaggacatc gcgaagatcg ccggcacctt ccactcctgg atcggtcaca acgcggtcgt
     9121 tgcgcgagcg gagcgagacg aaacgcccca cccctacgcc gacatccccg gcttctgcta
     9181 ctccgcaact ctcgccgaga tcaaggccgc tgactacgcc ctgacgccgg gccgctacat
     9241 cggagcaccc gaggccgagg atgacggcga gccgatcgaa gagaagctcg ctcgcttgcg
     9301 agctggcctc gaggcacagt tcgcagagtc cgcgcggttg gcggacgtcg tacgcgagca
     9361 gctggggagg atctcgtgag gcttgatgcg gtgtgcagtc acgttgtcga ttgcgagcac
     9421 aagacggcgc cgatcgatga atcgggcgag tactttgcag taggaactcc ggcgatgcgc
     9481 gatcaccgga ttgaatacgc cgaagcacga cgcatctcac gcgacacgtt cgaggcgtgg
     9541 acacgacgtc tccgtccgca acccggggac ctgttgctgg cccgagaggc gcccgtcgga
     9601 ccaatcgttc gtctgcctga gtccgcccat gttgcgccgg gacagcgcac cgtgcttctc
     9661 cgaccggatg cggcggccac ttcaagcacg tatctctatt acctgctacg ttcgccgcgc
     9721 atgcaggcgg agcttctgct caaggctgag gggtctacgg ttcatcatct gaatgttgcg
     9781 gatattcgcg cactggaggt tgatctcccg ccccttgcgg agcagcaagc catcgccgag
     9841 gtgctgggcg ccctcgacga caagatcgcc gccaacaccg ccctcgccga gagtctcacc
     9901 aggctttcgg ccttggaatg tagggcgttg gcggcgagca caacgcggtc cgtcccgcta
     9961 gccgacctag tcaccacgca gtatggcgtc acgacgtccg ccgtcgtgga gccccaaggg
    10021 acgggtcttg tgagggttac tgacatcaac aagcggccct ggatcgagtg ggacagtgtt
    10081 ccgggttgtt ccctagacaa gggggtaaga gagaagtacc tcctcaaccc tggagacatt
    10141 gttgtggccc gcatggctga tccaggaaag gcggcttgga tctcgctggg cgaccctcct
    10201 gccgtgttcg cttcctatct ggttcgtctg caggcaacga tcgagtatca cgccccgttt
    10261 atttacgagt tcttgcgtgg tgagcaatac cgtcgatatt gcgaaggagc gatgcagggt
    10321 tccgtccaga agaacatgaa cgccaaggtg atcgtcggca caaccatgcc gctccctgac
    10381 cgggcagcgt tgagagaata tgccgggagg ttccacggcc tgcgggcgtt gatgtcatcg
    10441 gcgcttcgcg agaaccgcac cctcgctgcc acccgcgacg cactgctccc gcagctcatg
    10501 tcgggcaagc ttcgcgtccg tgacgccgag cgcatcgcaa cagaagcagg cgtgtgatgg
    10561 ccttcgccgt tgtcgatttc gaaaccaccg gcatcctgcc ttcgtaccac catcgcgtcg
    10621 tggagatcgg cctgacccac gtcgaggacg acggcacgat ctcgggccgt tgggagacgc
    10681 tgatcaaccc ggaccgcgat ctcggtccgc agcggatcca cggcatccgt gccgccgacg
    10741 tgctcgacgc gccgctgttc agcgacgtcg cggccgagtt cgccgaactg ctgcaaggtc
    10801 gggtgtttgc ggcgcacaac gcgtccttcg acttgcgctt tcttcaggcc gagttcgagc
    10861 gcgccggcta ctggctcgac ggcggcaccc cgagcgtgtg cacgatgcgc ctcggtgcca
    10921 gcttcggcct cggcgcctcc ggctccctcg tccagtcctg cagcgcctgc gcgatcgacc
    10981 tcacccacgc gcacagcgcc ggggccgact cccatgcggc agcacaactg ctcgcgcact
    11041 accagcgcag cagcgcggac tggccggggt ggcccgccta ttgggagcag acggcggacg
    11101 cagggcgcag gtatcgatac ccaaccggct cgcggacggg tatcgtcgcg aagccgcggg
    11161 ccgaggcatc cgcaacgcca cccagctttc ttgagcggat ctcgaccgag gccgtgcgtg
    11221 agaccgtcga cggcgcggca gctcagtatg tcgcgttgct tgatcgctgc ctgctcgacg
    11281 gcttcctctc atcgagcgag aggagccagc tcgccgttgt tgccacggaa ctcgggctag
    11341 atcgggcgac tgtcgagggc ctgcaccgtg actacttcgt caccctggaa cgccgcgcct
    11401 gggcagacgg cgtgctcact gaagacgaga agaccgatct cgctgcggtg ggcatgctgc
    11461 tcgagctcgg agatgacgcc atcgcggccg cgatcgatga tctcgcccca gacggcgtcg
    11521 acgaagacgc gcaaacggcc ttcactctga acccgggcga tctcgtcgtg ctcacgggag
    11581 acatgtcgcg tccccgctcc gactgggaag ccgagctcca ggctcgcggg ttcatctgcc
    11641 accccgccgt gacaaagaag gtctcgctcg tcgtcgccgc ggatccggat tcactgtcgg
    11701 ggaaggcaaa gaaggctcgc gactacagca ttccgatcgt cagcgagcac tggctgaagg
    11761 agttcctgca atgaccacca ccacaccgcg acgcacggca cccgcctggg cgaaagtcct
    11821 cctggtcgtc gtcatcctcg ccctgatcgg tgctgcagca ttcgccggtc tcacgatcgg
    11881 caagttcctc ggcgcgagtg aggagcgcga tgttcaggtg gtgcggtccg ttcagggaga
    11941 ggagcaggtg atcctcctga ccgccggact caccgacata aaagagaagc acggcaagca
    12001 gacgttcttc gggctcttcg acgtgccgtt gtcagatcgc acgtcattcc tccggtacga
    12061 gttcgaagcg aaattcggaa tcgaaggcaa gcaggtcgac gtcgagccgc tcggagacaa
    12121 ggcatatcgc atcacgattc cggagttcat cttccttggc tacgacgacc cggagttcag
    12181 tgtcgcaacc gaatcgaacg gcatcctgag ctggacgacc ccggcgatcg acacgctcaa
    12241 ggccaccgaa gaactcctca gcgacaaggc cgtcgccgag cacatcgacg ggttccgtcc
    12301 gctgctcgag gaacaggcga tcacgttcta cacgcgcatc gtttccagta tcgaacctga
    12361 cgcgaaactc accttcgagt tcagcgaatg acttcacact ctcacttcga atgaggaccc
    12421 ggttatgacc gaaaacgcag aagaagcgcg cacatccgaa cccggcgcgc gtgattccat
    12481 tcccaagatc gacgagcgcc cctttctcga cgcgacctcc gaggcgaagt cccgctatgg
    12541 ccgtttcaat ctcgccgtca tcggcagctc aggtgtcggc aaatcctcgc tcgtcaacgc
    12601 cgtgttcggt cgcgactggg cgaaggtcgg caagggcctg ccggtcacgc gcggcgtgca
    12661 gtactaccac gacgactcgc ttggtatctg ggacgtcgag gggttcgaga tcggctccgc
    12721 cgtcgcgccc cgcgaacagc tgcgtgcaca cctgaacgtg atcgcgcagc atcccgccga
    12781 tcagcagatc gctgtcgtct ggtactgcgt gaagtccaac gatgaccgcc tgacggccgc
    12841 cgatatcgag atgatccgcg agctcgatgc gcaaggcctg ccggtgatcc tcgtgctcac
    12901 caaggtcgac gtcggcggca gtgtcattcc cggcaagcga acgatcccta agggggtaca
    12961 gcagttcatc gactggctcg agagcccggt cgacggagag gaccagcagc tgcacattcc
    13021 gtaccagcgg gtgatcgtga ccgccgtccg agacaagaac ggaaaggact cgggatacgg
    13081 tctcggtgag ctcgtcacag agactctcgc actctctccc gaggacgaga aggatgcctt
    13141 tcgcatcgca caacggctca accttccgtg gaagcgcgag atggctcgca cggtcatcgc
    13201 gggagcgtcg accgcagcag ccggagccgc cgcggttccc gttccggtcg cggatgccgc
    13261 tattctggcg ccgatccagc tcggaatgat gggcaggatc gcaacgatct acgacctcga
    13321 catccggacc atgctgtctg ccggtaccct cgcgcaggtc ggcgtgcagc tcgccggtcg
    13381 cgcactcgcg agcagcttcc tcaagctgat ccccggcgcc ggaagcgtag tcaacgccgg
    13441 tgtcgccgct gcgttgacgg gcgcgaccgg tgagggatgg atgcgtctgt gcgaacaggt
    13501 gcacactggc aagctcgacc tcgcggccat caaccagtca ttcggcgact atgttccggg
    13561 gttcctcgac gtcatccgca agatggcgga gcagaagctg agcaagtcat gacccgccca
    13621 tcgagcacga gcaacgacgc acacgaaggg tcttcatcat gacgacatac tcggaatcgc
    13681 agtgggaaca ggacgccctg gacatgctcg ccgagccgct cggctggcgc ccgctcactg
    13741 gagagcagat cgcgcctggc actgacgagc gggactcctg ggacgacctg ctggtacgcc
    13801 caaggctgct cgacgcactg cggcgcctca accccggcat ccccggtgag taccttcagc
    13861 aggcgctggc cgagatcgca tcgccgaaat cacaggacgc gatcactgag aaccaccgca
    13921 tccaccagta cctcgtcggt ggatacccgc tgagctatct cgacgccgat ggagtcgagc
    13981 agaacccgcg gctgcgtctg ctgggctccg acccctccga gaacgactgg atcgccgtca
    14041 atcagctcac tgtccggcag ggcgatctgc acaggcggtt cgacgtcgtg ctgttcgtca
    14101 acggcatgcc cctcgtgatc atcgaactga aaagagcggg ctcggcatcc accggtgtcg
    14161 ccccagccca cgcacaacta cagacttacc ttcgcgagtt tccgatggtc ttccgattct
    14221 gcgtgctcac actggccagc gatggtctcg aagccaagta cggaacacct ttcacaccgc
    14281 tcaaccactt cgcaccgtgg aacgtcgacg acgacggtgc gcccctgacc cagccgcggg
    14341 tcgtcggtgg tgtgtccgtc gagccgatcg acgatgcgct cggcggtctt ttcaaccagg
    14401 agcggttcct gcaactggtg cgaagcttca ccgcgttcga cgagaacgcg gacgggctga
    14461 ccaagcgcat cgccaagccg caccagtact tcgccgtaac gaaggccgtc ggcagtacgg
    14521 tgcaagcggt cgagacgaac ggcaaggccg gcgtggtgtg gcacacccaa ggctcgggca
    14581 agtcgatgga gatggagcta tacacgaatc tggtgtcgcg gcatccgaag ctcaagaatc
    14641 cgaccgtgat cgtcgtcacc gaccgcaccg agctcgacgg ccagctgttc cagacgttcg
    14701 accagagcct gttgctcgct gagaagcctg tgcaggtgcg cgagcgggcg cagctgcggg
    14761 acgagctgag caaccgagtc accggcggca tctacttcac gaccctgcag aagttcgggt
    14821 tgaccgaggc cgagaagcag tcgggcaccc agcatccgct cctcaccgat cggcgcaacg
    14881 tgatcgtcgt cgtcgacgaa gctcaccgca gccactacga caatctcgac ggctacgccc
    14941 ggcacctgcg cgacgcgctc ccgagcgcga cgctgatcgc cttcaccggc accccgatct
    15001 cgttcgccga acgagacacg cgcgcggtgt ttggcgacta catcgacatc tacgacctca
    15061 cccgcgccgt cgatgacggt gccaccgtgc cggtgtattt cgaaccgcgg ctcatcaagg
    15121 tctcgttcgc cgagggttca accgaagagg acatcgatca ggccgccgac gagtacacgc
    15181 tggggctcga tgacaccgaa cgggcacgcc ttgaggcatc cgttgccgtc gtaaatgctg
    15241 tctacggtgc cccggaacgc atcgacaccc tcgccgcgga cctggtggcg cattgggaag
    15301 aacgtcgtga gcggatgaag ccgttcctgg agtcgccagg aaaggcactc atcgtcggcg
    15361 ggacgcgcga gatctgcgcc cggctctatg aggcgatcgt ggcgctgcgc cccgattggc
    15421 actccgacgc gctcgacaag ggacgcatca aggtggtgta ctccgggtcg gcatccgacc
    15481 agcctccagt aagcgaccat gtccggcgtg actccgagaa caaggcgatc aaggcgcgcc
    15541 taaaggatgc aactgacgaa ctcgagctcg tgatcgtcaa ggacatgatg ctgaccgggt
    15601 tcgatgcccc gccgctgcac actctgtatc tcgaccggcc gctcaagggc gcactactga
    15661 tgcagacttt ggcccgagtg aaccgcacgt tccgaggcaa gcaggacggt ctgctcgttg
    15721 cgtacgcgcc gctcgcagac aacctcgcca aagctctttc ggaatacacc gtctccgacc
    15781 aggagaacag gccgatcggc cgcgacgttg acgaagcggt cgccatcgcg aacgaactgg
    15841 tagcgcagat tcgcgagatg ctcgccggct gcgactggcg gtcgatcgtg aaggcgggtg
    15901 gggcgcgccc ttggcgcacc gcagcgacga cagctacgaa cttcctgcgc gatccgggca
    15961 acccgctcaa tgccgaggag cttggacccg agcgccttgc cgcgcggtac cgcaccgcaa
    16021 ccggtcagct cagccgagcc tgggctctcg catcggggcg cgacggcttg gccgagctgc
    16081 acgaggagat cgccttctac gaagaggtgc gcgtgtggat ggcgaagttc gacgccgccg
    16141 aacggcagtc acgcgacgaa ccgatcccgg aagagatctc acgactgctc ggttcactcg
    16201 tcgcagatgc aatcgccccc ggtgacgttc tcgacatcta tgaagcggcg gggcttccgc
    16261 gcatcgctct cgacaatctc ggtccagaat tcctcgcgaa ggcgcaggct gcgccgaacg
    16321 cgcacctcgc gatcgaagca ctgcgggcct cgctgacgga tgccagtgcc actgcgacca
    16381 ggggaaacct cgtgcgccag cgagcgttct ccgagcggat cgccgagatc atgaaccggt
    16441 acaccaacca gcagctcacg tcagctgaga tcatcgcgga gctcgtcgaa ctcgcgaagg
    16501 aggttgcgga ggaagccgct cgcggagcaa acttttcgcc cccgctgtcg aacgacgagt
    16561 tggcgttcta cgacgccgtc gccacgaacg attcggcgat cgacatccag ggcgaggatg
    16621 tcctcgcgca gatcgctcgc gagctcgtcg ctgtgatgcg gcgcgacgtg cgtaccgact
    16681 ggactgtgcg cgacgacgtc cgtgcgaaac tgcggtcatc gatcaaacgg ttgctcgtgt
    16741 tctacaagta cccgccggac aagcagcccg aagcgatcaa actcatcatg gatcagatgg
    16801 agtcgatggc gccgcgatac gccgacgagc gcagggctcc caaccatgaa tgacacgctg
    16861 tgcttcactc gggcgcttcc gccacagccg gttggcattg aggctgcgac gcgagaagtc
    16921 ggaggtcagt gggactggtg ttgggcaatc ccggcgcctt accgcgtcct agccgcgccg
    16981 aacgcttggc ttccgatgcc agccgctccc cgtcccaatg aggacttcat gcgctcgctt
    17041 ggctattggg cgtgcctgca atcgttcttg acgttttcct tcgcctggac tcgccatgac
    17101 agaggactcc tctggtggta cggcgagggc atgcccgtcg aggatccgcg ctttcgccta
    17161 cttcgtgatg tgtggcatgc agatggcatg ctggagcgat acctcgagtg ggtgattgat
    17221 ggacatcacg tgtcagaacc actacggggt cacgcactcg ctatcgatga acttcccgtg
    17281 cgcctatcag cagagtggaa tcgtcgggtt gaggtcgtgc gccaagaaga tctgaccgac
    17341 tccacagtcg ggcgcactcc acacggtaag cacctcgagg gcggagggca tattgccgga
    17401 ccagccatga gagttgagaa ctctgacagc gtactttttc gttcgagttc aggtgcgctc
    17461 ctctcggtgt cgtctgtccc cggctggtat gcggacctca cgcgcctcgg aaacgctgcg
    17521 cagggtatgg cgcgcacatc ggttcgcgtc gatgtacttg tgaagccgat tggcttcatg
    17581 ggcacgttcc gccgatctcg cacgactggc ctttggttta ccggcgcccg tcgctaccac
    17641 tcggtaggga attaagagcg gcccatcgcg aatggcgccc tgcctacgat tcctatgctg
    17701 ccgccattga ctgctagcga ataggctcca accaaactca gcacccaatc ccccgcagca
    17761 tcacatcgac tatcccgtcg gccttcccgg cttccgtcgg cccgcccacc agtgccgagt
    17821 acgagccgat cgcgatcagc tggtcagcca ccaccgcggc atccacgtcc gggcgcacgt
    17881 ccccggcatc caccgcctgt gccagccgca ccgcagtcca ctcgcgaatg ggcgccgcca
    17941 ggcgcgcgtt gagcttcagg ccgagctcgg catccgtcgc agtcacttcg atcagggcgc
    18001 gcgcgattgc gacggtatcc gggttctgtg cggacaacac cgcgcgcagg tcggcgcgca
    18061 gatccgacgt cataggcaat tcgaaaccag gaagcgaacc ctccagcagc gcctccccca
    18121 ggatcgccgc tttcgacggc caccagcggt agatcgtctg cttcgagacc ccggcctccg
    18181 ccgcgagtcc ctcgatcgtg acggcggcgt acccgtccgc cgcgagcgcg cgcagcatcg
    18241 cctccagcac cgcatggcgg gctttctcac tgcgaggacg gggcacggga tcagcgtatc
    18301 gggcgtagag tggaattact ggacgatgcg ttgcgttatc gcttcgacag gctccgagac
    18361 tcgaccaagg gaacgaggaa gaaatggcac tgaccgcgca ctccaccatc ggcgagtgga
    18421 tgaatgacgc caccggcggc ccgctcatcc gaggtctgtt cgagaagacc ggcgccgacc
    18481 ccgaactgct cacccccgtg ctcggcctcc ctctgcagca gctcgtcgcc atgagccagg
    18541 gcgccctgcc ccagtcggtc gtcgacgatc tggtccgcgc ggcgaacggc ggcgaggtcc
    18601 ccgaagacac cgtgcccacc ggctgggtcg agaacatcac cccgggtcgc ttcaccggca
    18661 agaccgtgat cgtcacgggc gcggcatccg ggatcgggcg cgccacggca tcccgcatca
    18721 cccgcgaggg cggacgcgtg atcgccgcgg acatctccgc cgacaagctc gacgccctca
    18781 aggacgagct ccccggcatc gtcaccgtcg ccggcgacct cacccagcag gacgcgatcg
    18841 acgccgtcat cgccgccgcg ggcgaccaga tcgacgggct cgcgaacgtc gccggcatca
    18901 acgacgactt ctcgcccgcc ggagagacca cggatgccgt gtgggaccgc gtcatcgcga
    18961 tcaacctcac cgccccgttc aagctcatgc gcgcggtgat ccccgtcatg gagaagaacg
    19021 gtcgcggcgc gatcctcaac gtttccagcg aggccggcct acgcggcaac gcctcgggca
    19081 acgcctacac ggccagcaag cacggcatca tcggcgtgac caagtcggcc gcggtcatgt
    19141 acggaccgaa gggcatccgc gtgaactcgg tcgccccggg tggcgtcgcc accggcatcc
    19201 cgatgccgcc gcacatgtcc gagtacggat cggcccgcct cgccccgttc cagcaggcga
    19261 tcccgagcgt cgcgaccgcc gagcacctgg cggcgtccat caccttcctg ctcagcgacg
    19321 acgccgtgaa catcaacggc gcggtcctgg catccgacgg cgggtggtcg gtgcagtagg
    19381 caccggacac gggtcggaag cgcggattct cctgagcccg cgcttccgac ccccgctcac
    19441 gccgcgtccg cggcatcccc tcccgcatcg atcgcgcggc ggttgctggc ctgctcgatc
    19501 cccttcagcg tcttgtcgtc cgcggtgcgg tgcagcgcct tgcgatacgc cttgcggatc
    19561 tcggccgcga cgaactcggt gaggtagccc cacggatcct cggtgaccat cagccaggtg
    19621 cgctcggcgc cccaggtgtt gtcctcgagc acctcgagca cctcgagcac gatcgcggcg
    19681 acaccgcgag ggtcgagcgt ggcgccgacc tccgcgagca cgcgggcgcg gatctcgggc
    19741 acgatgcggc gccgcacctg cgcgaggaag cgatcgcgct catcctgatc cacatcaacg
    19801 ccgcggttga aggcgtcccc cgagcgcagc gcggtgagga aagtgcggag ctggtcgttg
    19861 ctccagtcga tttcgggcat ggcagagact cctgtcgtga gtgaacgatg gcaagaagga
    19921 cgggccgctc agcggccccg cagtcacgac tgcaggtctt cttgcctcgg tagctctcga
    19981 cggacggatg ctgcccacgc agacgtccgc gcactctgac cagcgccctg gcgggcggcc
    20041 ctgcgagtgc ggacggcgga catgagatcg accgtacaac cgggtgagac atcgatcaag
    20101 agcgggcgtg gatcacgacc gctcgatgcc ggcggaggcc gttgccgccg tggacgggct
    20161 gctcacctca tccgggatcg tgcacgacac ccgcatccgc cccgacgccg cgcacgggta
    20221 cacgatggcc gacacgtcct cctacgacga gaccgccgga gactggcact tcacacgcct
    20281 tcgagagtct cttcccgact gaccgggctc ctgcgaacga gcatccgcac caccgtcccg
    20341 accacgccga ccacaagagc gacgacgacg ggcgcgacgc cggcgtgcag catcggcacg
    20401 aagtactgtg cgatcgcgcg cgggatctcc tgcggatcat tctggatcgc ccggatgccg
    20461 accgcgtacg agatcgaggt gatcgcggcc ggcatcaccc acagggccgc cagcccgatg
    20521 acccacacgc tcagccgccc ccacgggcgc accccgcacc acaccagcgc gagaccgacg
    20581 atcaccgccg gcagccaccg cagcacgatc gccagccact ccggatagct gtcgatcgac
    20641 acgaacacga cgaggggtcc gccgatccag ttcgtcacca gcggagcggc gagcgccacg
    20701 gcgagcgcgc cgaacgccgg agacctccgg gttgcgaaca gcagccccag ctgagcgaag
    20761 agcagcccga cgacgaccac cagaaccaac ccgagcaggt acagcagctg ggtgccgtcc
    20821 atgagcatcg cgccgaccgc ctgctccgag ccgccgccga tccccagccg tccgagcagc
    20881 gcaatgaagc tctgcaccgc cgccgccaac tgcgccagca gcacacccca cgctgcgggc
    20941 cagacctgca ctggacgcct gcggcgcagc atccgcacga tcagccccgc gagcacaccg
    21001 cccagcacga gcgtcgcgaa cagcgggaac acatggtcga gatcgagggg aaggaacgcg
    21061 aacggccttc ggccggtcag tcgctcggca tcccaccgcg tgggcacctg gagtcctcca
    21121 cccgtcagcc agccgggcac catcacgacc agcgccgcgc cgacgccgat gaggaacgac
    21181 ggccaccgcg acggcgtcgt cgccgtgaca tagggattcc gcctgtcgga tgccatgagg
    21241 cgagtgtccc agaggatgcc cgcaggcgcg acggcttcgc ccttcatcgg agaacggaga
    21301 ccatctgcgc ggcgcgcggc gtgtcagccc ggaaagcgcc gtccgtcgct cgactggccc
    21361 gggttctccg tgccggatgc ggggcggctg gcgccgcgac gcgccggtag actggccgtg
    21421 ccccgccttc ctcttcttca ggagcccgcg tgtccgccga agcgccccag accactcacg
    21481 tccccgactc cgtcgagaac gccatcgcga cccctgagaa ggagcagccg tacgcggcgc
    21541 tcggcctcaa ggacgacgag tatgcccgca tcaaggagat cctgggccgc cgccccacct
    21601 cgggcgagct ggcgatgtac tccgtgatgt ggtcggagca ctgctcctac aagtcgtcca
    21661 agaactacct gcgccgcttc gggcagaagg tctccgacga gatgaagcag cgcctcatgg
    21721 tcggcatggg ccagaacgcc ggcgtcgtcg acgtgggcga gggctgggcg gtcaccttca
    21781 aggccgagtc gcacaaccac ccctccttca tcgaaccctt ccagggtgcg gcgaccggca
    21841 tcggcggcat cgtccgcgac atcatctcga tgggcgcccg ccccgtcgcg gtcatggatg
    21901 ccctgcgctt cggcgccatc gaccatcccg acaccccgcg cgtcgtgcac ggcgtgaccg
    21961 ccggcatcag cttctacggc aactgtctcg gcctgccgaa catcggcggc gagacggtct
    22021 tcgacgccgt ctaccaggcc aacccgctcg tcaacgcgct cgcggtcggc gtgctccgcc
    22081 acgaggacct caagctcgcc aacgccacgg gcgtcggcaa cctggtcgtg ctgttcggcg
    22141 cccgcacggg cggcgacggc atcggcggcg ccagcatcct ggcatccgac tccttcgacg
    22201 acggcggacc caccaagcgc ccggccgtac aggtcggcga cccgttcgcc gagaaggtgc
    22261 tcatcgagtg ctgcctggag ctgtacaagg gcgagctggt cgaggccatc caggacctcg
    22321 gtgccgccgg catctcgtgc gccaccagcg agctggccgc caacggcaac tcgggcatgc
    22381 gcgtctcgct cgacaacgtg ctgctgcgcg accccaccct gaccgctgag gagatcctca
    22441 tgtcggagtc gcaggagcgg atgatggcga tcgtcgcccc cgagaagctc gacgccttca
    22501 tggccgtcgt gcacaagtgg gaggtcgaga ccagcgtgct cggcgaggtc accggagacg
    22561 gacgcctcgt catcgactgg cagggcgagc gcatcgtcga cgtcgacccg tccaccgtcg
    22621 cggtcgacgg tccggtctac gaccgtccgg tcgcctaccc ggcgtggatc gacgcgctgc
    22681 aggcggatgc cgcggagctg ctgccccgtt cggatgacgc cgcgaccctg cgtgagcagt
    22741 tcttcaagat cctcggctcg ccgaacctgg ccgacaccag ctggatcacc aaccagtacg
    22801 actactacgt cggcggcaac accgccctcg ccttccccga cgacgccggc atgatgcgcg
    22861 tcgacgagga gtccggcctg ggcttcgcga tcgccaccga cgccaatggc cggtactgtc
    22921 agctcgaccc ctacgcgggc gcgcagctgg ccctggccga ggcgtaccgc aacgtcgccg
    22981 tcaccggcgc gaaccccacc gccatcaccg actgcctgaa cttcggctcc cccgagaacc
    23041 ctgaggtcat gtggcagttc gggcagacgg tcgacggcct ggccgacgga tgctacgagc
    23101 tgcgcacccc cgtgaccggc ggcaacgtct ccttctacaa ccagaccggc gacgtgccga
    23161 tccaccccac cccgctcgtg ggcgtgatgg gcatcatcga ggacgtgtcc cgccgcatcc
    23221 cgtcgggctg gcaggacgag gggcagaaca tctacctgct cggcaccacc tcgaccgagc
    23281 tgtccggctc ggcctgggcc gacgtcgtgc acgaccacct cggcggtctc ccgccgaagg
    23341 tcgacctggc gcgtgagaag gacctcgccg gtctcatctc cgccgcccgc gacgagtggc
    23401 tgatctcgtc cgcgcacgac ctctccgaag gcggcctggg ccaggccctc gccgagggcg
    23461 tgatgcgctt cggcgtgggc gcccgcgtct ggctgaccga gctcatggag cgcgacggtg
    23521 tggacgccgc atccgctctg ttctccgagt cgaccggccg cgtgatcgtg accgtgcccc
    23581 gcgaggacga cgtgaagttc cgcgggctgt gcgagggccg cggctacccg gtgctgcgca
    23641 tcggcgtcac cgactccgag cccgcgctcg aggtgcagga cgtgttcacc gcgaccgtcg
    23701 ccgagctgcg cgaggcctcg aaggcgaccc tgccggcggt cttcggtccg acggtcaccg
    23761 agccggtggc cgtgtgacgg gcacgatgaa ccgcaccgac gaggactacg gcgatctcgg
    23821 cgagcgccgc aaccgccgca tccgcctggt cgcctgggtg accatcgtcg cgctcatcgt
    23881 cggcggcggc ggcgcgaccg ccctgacgct gctgctcggc tgagaccgcg atgaccaggc
    23941 gcaagcgacc ctacaagagc accgcgatcg tgctctgggc gctcggcgtg atgctgacgc
    24001 tgcccgcgct gctgcccctg gcatccggcc cccccggtgc acaggccttc gctccgatcg
    24061 gcatctcgat cggcgtgctc gtgctgatcg ccggcacgct gctgttcttc accggcccga
    24121 aggagccgct ggaggacccc cgcgaagcgc ccgtcgacga cgactagagt cggatgccgt
    24181 tgggccccga gcctgtcgcg gatgccgttc accttcgccc ttgaagggcg tagcctcggc
    24241 cgggaacgga ggcaccatga ccatccagat cgccgacgtc gcccacgacg cgaacctcga
    24301 ggggcatctg atcggctcgc ccgtcagcat catccgcgag tacgccacca cggcgggcgg
    24361 cgggccccgg ctgcaccggc atccgtacgc cgagacgttc atcatccatc gcgggagcgc
    24421 cctgttcacc gtcggcgagg agcagctcgt cggggtcggt gggcagatcc tcgtggtccc
    24481 ggctctcgtg tcgcaccgct tcgaggtgct cgacggcggc tacgaggcca cgcacgtcca
    24541 tgcgagcgag cgattcatca ccgaatggct ggaatagccc gctccgccct gcgatgttgt
    24601 caaccctctc gtccaggccc gccgaagctc ggatgctggc ctccaccgat caccgactgg
    24661 aggctgacat gagcgacagc acaccgaacg acgaaccgct gagcgacgaa ccgctgaacg
    24721 acgatcccct ggagaacgga ctcgacaagg gctcgggcga tccgaacgac gaccgcgact
    24781 tcctcatgga ccagccgccc gcgcaggtga agccgcagga ggatcgcgac gacgaggacg
    24841 cgtcccgctg agcgggcgcg gtccgcgcag ctcggcatcg cgtacgaggg cccggcatcc
    24901 gatcgacgga tgccgggccc tcgccgtctc tgcaggcgcg ctcagccgac ctcggacttg
    24961 cgtccgccgg cctccagcga gtcgcccgcg ggcagctcct tgtggccgcc gaactgcagg
    25021 cgcatcgcgg agagcacctg gttggcgaac tggtcctcgt cacgcgaggc gaagcgctcg
    25081 aacagggatg ccgcgagcac cggcacgggc acgcccacat cgaccgcggc cttgaccgtc
    25141 cagcggccct cgcccgagtc ggagacgcga ccggcgagtc cgtcgagcga ggggttctcg
    25201 ttcagcgcgg tcgccgtcag atccagcagc cacgacgaga tcaccgaacc gcggcgccac
    25261 agctcggtga ccttcgcggt gtcgatgggg aactggtagt actccggctc ctctagcggg
    25321 gcgatctcag ccgagtgctc ggcctcgcgc acaccggcat ccgcgttctt caggatgttc
    25381 aggccctcgg cgatcgacgc catgatgccg tactcgatgc cgttgtgcac catcttcacg
    25441 aagtgcccgg cgcccgacgg gccgcagtgc aggtagccct gctcctcggg ggtgagctcg
    25501 ccggtgcgac ccggggtgcg ctcgatctcg ccgacgcccg gcgcgatggt cttcagcagc
    25561 ggcccgatgt ggttcaccgc agcatccgac ccgccgacca tcaggcagta tccgcgctcc
    25621 aggccgaaca cgccgccgct ggtgccggcg tcgacgtact cgatgccctt ctcgcgcagc
    25681 gccttggcgc gacgcacgtc gtcgcggtag ttcgagttgc cgccgtcgat caggatgtca
    25741 ccgggctcga gcacctcggc gatctggtcg acgaccttcc ctgtcagcga agcgggaacc
    25801 atcatccaca cgacgcgcgg ggtctgcagc ttggctgcga agtccttcag atcggatgcc
    25861 ccggtcgcac cctccgcggc gagcgcagcc acggcatcct gattcacgtc gtaggccacg
    25921 cactcgtggc cgtccttcat cagtcggcgc acgatgttcg cgcccatccg gccgagtccg
    25981 atcattccca gctgcatgtc ttctcctcgc gtcgttccag ccttcccatc atcctccctg
    26041 cggcgcaggt tcgggaggag aacgcacaga ggggaggagg atgccgtgca catcctcctc
    26101 ccctcacgga gaagtcctcc cgaacgagag cgcggcgatc agccctgcgc gttcgccccg
    26161 gcgatcagct gctcgatctg ctcacgggtc accgggatac cggggaagcc gacgatgcgc
    26221 ccgatcggga acgaggccat catcttctcc atgccgtcgc cctccatcat cgaggcgccc
    26281 gcggcatcct gcggctcggc gagccccgcc agcgcctgct gcacgatggg acccgcgaac
    26341 gggtgcgcca tcacctcgcc gatcgaggag ttcatgtcca gcggcaggcg caccgcgtcg
    26401 ccctcgagac cgaccgccgc cgaggagcgg atgtcgcggc tggacgcccc gacctcgatc
    26461 acgtactcgc cgccctcgac cacgaagcgg tcgacccgca cgtcccagta cgccaggtcc
    26521 tcacggcgga tgaccagctc gaccgccacg gtctctccgg ctgcgacggc caccgacgcg
    26581 aacgccttga gctcgcgcgg ggcgcgctgc acgagagagc cgggcagtga ggtgtacgcc
    26641 tgcacgatct cgcggccgtc gcggtcgcca gtgttggtca cctccagcgt cacgacgatg
    26701 tcgccgtcgc gcacggatgc cgtggcgtcg ccgtaggcga acgtcgtgta cgacagacca
    26761 tgaccgaacg ggtaggtgac ctgcaggtcc ttcgcgtcgt accagcggta gccgaccagc
    26821 agtccctcgc cgtagcggac atggccgaac tcaccgggga agttgccgta ggaggggttg
    26881 tcctccagac gcaccggcac ggtctcggtg agcttgccgg aggggttgac cgcgccgtac
    26941 agcacatcgg cgaccgcgcc tcctccggcc tggccgagca gccaggtctc gacgatcgcc
    27001 ggcacgcggt cggcgaaggg cagcgccacc acgccgccgt tggagagcac gaccaccgtg
    27061 ttcgggttcg cggcgaccac ggcatccaga acggcgagct gctcggccgg gaggtcgatg
    27121 tgctcgcggt cgaagccctc tgactcctgc gaggcaggca gccccaggaa gaccacggcg
    27181 acctctgcgg cggacgcggc ggcgaccgcc tcgtcccgca ggtcgtcggc ggaacgaccg
    27241 gatgccgcga tcgctccacc cttgacggcg aagccgggcg cgtacgacac agcatccccg
    27301 ccgacctcgc ggagcgcgtc gagcgcggcg tccactcggg tcgggttgat cagcgatgag
    27361 cccgccccct ggaagcgagg ctcggtggcg aaggcgccga tcaccgccac cttctgcgac
    27421 ggcgcgagcg gcaggatgcc gccctcgttc ttcagcagca cgatcgagcg actggcggcc
    27481 tcgcgggcca gcgcatggtg cgcgtcgacg tcgagagggc cggcgaccac cggtcgct
//
LOCUS       DATMSX010000401         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_53433,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000401 DATMSX010000000
VERSION     DATMSX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53433"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /locus_tag="VN041_12655"
     CDS             complement(<1..2248)
                     /locus_tag="VN041_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWU29923.1"
                     /translation="MSTLLSSLGRWSYRHPWRVLVSWLLVLGIAGGGALVLGSGTDNT
                     FSIPGTESQAGLQQLERTFPQVSGTNAQFIVVAAPGDDIRDAPYKNDIEHTISKLKDV
                     DDRVLAVSSPYDETVTGMINAEHSAAIVRIQLEGQSSNIPASVKTALQDTVNALGPEL
                     PKGSQVALGGDLFAMAIPTITLTEAVGLVIALLVLIVTFRSFVVAGLPLLTAILGVGI
                     SMAGIFAATAFATVSSTTPLLALMLGLAVGIDYALFIVARHQDQVRAGVDPEESAARS
                     VGTAGSAVVFAGVTVLIALIGLGFAGIPFLTTMGIAAAVAVAVAVAIAITLTPAFLGF
                     LRGRVAGRVRADRPAKKTKKDAAPKTRRPFSQRWVAGVTHRPILVTVAVVLGLGVVAV
                     PALSLNLALPNAGVLPKGSEARVSYDLTADEFGAGFNGPLILTGTIVTSTDPLNLMKD
                     LGDAVAKIPGVKEVALATPNETADTGIVQIIPTTAPDDPKTADLVRELRAHHDEWQKE
                     FGIDLKVTGFTAVAIDISDQLGAALLPFGVFVIGLSLILLTIVFRSIWVPITAAVGYL
                     LSIVAAFGVVAAVFEWGWFADALHVARTGPIISFMPIVLMGVLFGLAMDYQVFLVSRM
                     REDYAHALRQAQGPSSEDRREAARNAVRSGFSSSARVVTAAALIMFAVFVAFVPEGDS
                     SLKPIALGLAAGIAIDAFLVRMTLIPAVMAILGERAWRIPRWLERILPHVDIEGEAVE
                     RERSLAAWP"
     gene            2388..3005
                     /locus_tag="VN041_12660"
     CDS             2388..3005
                     /locus_tag="VN041_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU29924.1"
                     /translation="MSTPVTRSRENTRARLLEAAAQVFAEIGLEGATVEAVCERAGFT
                     RGAFYSNFESKDELFLTLAASVGEVRLGAVRERVSVLVGADALGDCDPVDLVQQIMDS
                     GADDRVGVMLMSEIRIRALRDPDFGTAYLAQEREMVRSIAAIIQEIVDTGKFGLRIDA
                     EEAARLLMIVWEGMTVRGAMGGDDSDRLRRVGSEALGRMVELMRA"
     gene            complement(3010..>3651)
                     /locus_tag="VN041_12665"
     CDS             complement(3010..>3651)
                     /locus_tag="VN041_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWU29925.1"
                     /translation="GQELADDVARVRAVRELMGPEGRIRIDANGGWNVDEAEHAIHAL
                     GDCDLEYAEQPCATVDELAELRYRVKHMGIPIAADESVRKAADPLAVAQAKAADLIVI
                     KAQPLGGISRALSIVAEAGLPAVVSSALDTAVGLAQGAALAASLPELDYDCGLGTASL
                     FADDVADARPVAGSISAARVTPDAAALARLEATSDRRAWWLARLERCHALLNA"
BASE COUNT          586 a         1192 c         1342 g          531 t
ORIGIN      
        1 ccggccatgc ggcgagcgac cgctcgcgct cgacggcctc gccctcgatg tcgacgtgcg
       61 gcaggatgcg ctccagccag cgcgggatgc gccaggcgcg ctcgccgagg atcgccatca
      121 cggcggggat cagcgtcatg cgcaccagga acgcgtcgat cgcgatgccg gcggcgagcc
      181 ccagggcgat cggcttgagc gaggagtccc cctcgggcac gaacgcgaca aacacggcga
      241 acatgatcag cgcggccgcg gtgaccaccc gtgcgctgct cgagaaaccg ctgcgcacgg
      301 cattgcgcgc cgcctctcgg cgatcttctg aactgggtcc ctgagcttgt cgaagggcgt
      361 gcgcgtagtc ctcgcgcatg cgcgagacga ggaacacctg gtagtccatc gccagtccga
      421 acagcacacc catcagcacg atcggcatga agctgatgat cggcccggtg cgggccacgt
      481 gcagggcgtc cgcgaaccag ccccactcga acaccgccgc gacgacgccg aacgcggcga
      541 cgatcgagag caggtagccg accgcggccg tgatcggcac ccagatcgag cggaacacga
      601 tcgtcagcag gatcagcgac agcccgatca cgaacacgcc gaacggcagc agggccgcgc
      661 cgagctgatc ggagatgtcg atcgcgacgg ccgtgaaacc ggtgaccttc aggtcgatgc
      721 cgaactcctt ctgccactcg tcgtggtggg cgcgcagctc gcgcaccagg tcggcggtct
      781 tcgggtcgtc gggcgcggtg gtcgggataa tctgcacgat gccggtgtcg gcggtctcgt
      841 tcggggtggc gagggccacc tccttcacac cggggatctt cgcgacggca tcgccgaggt
      901 ccttcatgag gttcagcggg tcggtcgacg tgacgatcgt gccggtgagg atcagcgggc
      961 cgttgaagcc ggcgccgaac tcgtcggcgg tgaggtcgta gctgacgcga gcttcggacc
     1021 ccttcggcag cacgccggcg ttcggcagcg cgaggttcag gctcagtgcg ggaacggcca
     1081 cgacgcccag gccgagcacc accgcgaccg tgacgaggat cgggcggtgc gtgacgcccg
     1141 cgacccagcg ctgcgagaac ggacggcgag tcttcggcgc agcatccttc ttcgtcttct
     1201 tcgccgggcg atcggcgcgc acccgccccg ccacgcggcc cctcaggaag ccgaggaagg
     1261 caggcgtcag ggtgatcgcg atcgcgacgg cgacggcgac cgcgacggcc gcggcgatgc
     1321 ccatcgtggt caggaacggg atgccggcga agccgaggcc gatcagggcg atcagcaccg
     1381 tgacaccggc gaagacgacg gcggaccccg cggtgccgac cgagcgcgcg gcggactcct
     1441 cgggatcgac gcccgcgcgc acctggtcct ggtgcctcgc gacgatgaac agcgcgtagt
     1501 cgatgccgac cgcgagcccg agcatgagtg cgagcagggg agtggtggag gagacggtgg
     1561 cgaacgcggt cgctgcgaag atgccggcca tcgagatgcc cacgccgagg atcgcggtca
     1621 gcagcgggag ccctgcgacc acgaacgagc ggaacgtgac gatcagcacg agcagcgcga
     1681 tcaccagtcc gaccgcctcg gtgagagtga tggtggggat ggccatggcg aacaggtcgc
     1741 cgccgagtgc gacctgcgac cccttcggca gctcgggtcc gagcgcgttc acggtgtcct
     1801 gcagggcggt cttgacgctc gccgggatgt tgctggactg cccctccagc tggatgcgca
     1861 cgatcgcggc ggagtgctcg gcgttgatca tcccggtgac cgtctcgtcg tacggcgatg
     1921 agacggcgag cacccggtcg tcgacatcct tcagcttcga gatcgtgtgc tcgatgtcgt
     1981 tcttgtacgg cgcatcgcgg atgtcgtcgc cgggcgcggc cacgacgatg aactgcgcgt
     2041 tcgttccgct gacctgcggg aaggtgcgct cgagctgctg caggcccgcc tgcgattccg
     2101 tgccggggat cgagaaggtg ttgtcggttc ccgaaccgag cacgagggcg ccgccgccgg
     2161 cgatgccgag cacgaggagc cagctgacga gcacgcgcca ggggtgccgg tacgaccagc
     2221 gacccagcga cgacaggaga gtggacacgc gacctccgca cgagttcgat acacgggtgt
     2281 attcaatacg tagatgtatc gtaagtcagg agcgaggccg gatgctgtgc gcgggatgtg
     2341 aacatccggc ggccgtctcg tgaaagagtt ggtccccagg aggagtgatg tcgacacccg
     2401 tcacacggag ccgcgagaac acgcgagccc gtctgctgga ggccgccgcc caggtgttcg
     2461 ccgagatcgg gctcgagggt gccacggtcg aggcggtctg cgagcgcgcc ggcttcacgc
     2521 gcggtgcgtt ctactccaac ttcgagtcca aggacgagct gttcctgacc cttgcggcat
     2581 ccgtcggcga ggtgcggctg ggtgcggtgc gcgagcgggt cagcgttctg gtcggtgcgg
     2641 acgccctcgg tgactgcgat cccgtcgatc tcgtgcagca gatcatggac tccggcgcgg
     2701 atgatcgggt gggcgtgatg ctgatgagcg agatccgcat ccgtgcgctg cgggatccgg
     2761 acttcgggac cgcctatctg gcgcaggagc gcgagatggt gcgcagcatc gccgcgatca
     2821 tccaggagat cgtcgacacc ggcaagttcg gcctgcgcat cgacgccgag gaggctgcgc
     2881 gcctgctcat gatcgtgtgg gaggggatga ccgtgcgcgg cgcgatgggc ggagacgact
     2941 ccgaccgatt gcgccgtgtc ggcagcgagg ccctcggccg gatggtggag ctcatgcggg
     3001 cgtgaacgct caggcgttca ggagggcgtg gcatcgctcc agtcgggcca gccaccatgc
     3061 gcgccggtcg gacgtcgcct ccaggcgcgc cagcgcggca gcatccgggg tgacccgcgc
     3121 ggcggagatg gaacctgcga ccgggcgcgc atcggccaca tcatcggcga acagcgaggc
     3181 agtgccgaga ccgcagtcgt agtccagctc ggggagggat gccgcgagag cagcgccctg
     3241 tgcgagtccg acggcggtgt cgagtgcgct ggagaccact gcgggaagtc ccgcttcggc
     3301 gacgatcgac agcgcgcgac tgatgccgcc cagtggctgc gccttgatga cgatcaggtc
     3361 ggccgccttc gcctgggcga cggcgagcgg gtcagcggcc ttgcgcacgc tctcgtcggc
     3421 ggcgatcggg atgcccatgt gcttgacccg gtagcgcagc tcggcgagct cgtccacggt
     3481 ggcgcagggc tgctcggcgt actccaggtc gcagtcgccg agtgcgtgga tcgcgtgctc
     3541 ggcctcgtcg acgttccagc ccccgttggc gtcgatgcgg atgcggccct cggggcccat
     3601 cagctcgcgc accgcgcgca cccgggcgac gtcgtcggcc agctcctggc c
//
LOCUS       DATMSX010000402         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_157994,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000402 DATMSX010000000
VERSION     DATMSX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157994"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VN041_12670"
     CDS             <1..414
                     /locus_tag="VN041_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29926.1"
                     /translation="RDRVRAPALTGVLLIATGAALVASGLSDFIDIKQGDYGTPALSL
                     IASAAISTGLILLAEGVVRPGRFSQVCTLLALPLLTIVLLHPVFLWTPWPAGPTFAAA
                     MLVPLVIGLVALRTPLSPWLTGQARLRRPQADPAR"
     gene            440..1369
                     /locus_tag="VN041_12675"
     CDS             440..1369
                     /locus_tag="VN041_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU29927.1"
                     /translation="MKSVPFGSGTAPAVIAGMMRIPDKTDAEVRELYDTARAAGIDFF
                     DHADIYGGAMHVCEDRFASALRLSAAERDEITLQTKCGIVPSEQMFDFSYEHIIAQVE
                     GSLKALRTDHIDVLLLHRPDALVEPEEVARAFDELSAAGKVRAFGVSNHTPRQIDLLR
                     TAVTQPLIANQLQLSITHSPIIAQGVAANMSGEEQSIVRDGGGIVEYCRINGITIQAW
                     SPFQAGFFDGVFLGNPKHPELNAVIDRLAAKYAVEPIAIATAWITRHPAHMQVVLGTT
                     TPQRVADAAAGADVELSRSEWYELFRAAGHIVP"
     gene            1413..>1679
                     /locus_tag="VN041_12680"
     CDS             1413..>1679
                     /locus_tag="VN041_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWU29928.1"
                     /translation="MNPLISAAELKDRLGEVRVLDARWQLGRDDGREQYLAGHIPDAV
                     FIDVEHELSRHGEPSEGRHPLPDDAALAEAARRWGISDGVPVVVY"
BASE COUNT          250 a          613 c          566 g          250 t
ORIGIN      
        1 cgcgaccggg tgcgcgcgcc cgcgctcacg ggcgtcctgc tcatcgcgac cggtgccgcg
       61 ctcgtggcga gcggcctgtc ggacttcatc gacatcaaac agggcgacta cggcacgccg
      121 gcgttgtcgc tgatcgcgtc cgccgccatc tcgaccggac tgatcctgct ggccgagggc
      181 gtcgtgaggc ccggtcgctt ctcacaggtc tgcactctgc tggctctgcc gctgctgacg
      241 atcgtgctgc tgcatccggt gttcctctgg acgccgtggc cggcggggcc gaccttcgcc
      301 gcggcgatgc tcgtcccgct cgtgatcggg ctcgtcgccc tgcgcacgcc cctgtcgccg
      361 tggctcaccg gacaggccag gctgcggcgg ccgcaggccg accccgcgcg gtagcgttgg
      421 cgtgaaccca ggaggaaaca tgaagtcagt cccgttcgga tccggaaccg ccccggcggt
      481 catcgccggc atgatgcgca tccccgacaa gaccgacgcc gaggtgcgtg agctgtacga
      541 caccgcccgc gccgcgggga tcgacttctt cgatcacgcc gacatctacg gcggcgccat
      601 gcacgtgtgc gaggaccggt tcgcctcggc cctgcggctc agcgccgcgg agcgtgacga
      661 gatcaccctg cagaccaagt gcggcatcgt gccctcggag cagatgttcg acttctcgta
      721 cgagcacatc atcgcccagg tcgagggatc gctgaaggcg ctgcgaaccg accacatcga
      781 cgtgctgctg ctgcaccgcc cagacgcgct cgtggagccg gaggaggtcg ccagggcctt
      841 cgacgagctg tccgccgccg gcaaggtgcg cgccttcggc gtctccaacc acaccccgcg
      901 ccagatcgac ctgctgcgca ccgccgtcac ccagccgctc atcgcgaacc agctgcagct
      961 gtcgatcacg cactcgccga tcatcgcgca gggcgtcgcc gcgaacatgt ccggcgagga
     1021 gcagagcatc gtccgcgacg gcggcggcat cgtggagtac tgccgcatca acggcatcac
     1081 catccaggcc tggtccccgt tccaggccgg cttcttcgac ggcgtgttcc tcggaaaccc
     1141 gaagcacccc gagctgaacg ccgtcatcga ccggctcgcg gcgaagtacg cggtcgagcc
     1201 gatcgccatc gcgaccgcct ggatcacccg ccaccctgcg cacatgcagg tggtgctcgg
     1261 cacgaccacg cctcagcgcg tggcggatgc cgcggcgggt gcggatgtcg agctcagccg
     1321 ctcggagtgg tacgagctgt tccgcgccgc cggccacatc gtcccctgag tgcccgtccg
     1381 gcgagcgaga cgtcccgagt gaggagcccg acatgaaccc tctgatcagc gccgccgaac
     1441 tgaaggaccg gctcggcgag gtgcgcgtgc tcgatgcgcg ctggcagctc ggccgcgacg
     1501 acggacgcga gcagtatctc gccgggcaca tccccgatgc ggtgttcatc gacgtcgagc
     1561 acgagctgtc gcgccacggc gagccgagtg aggggcggca tccgctcccg gacgacgccg
     1621 cgctcgccga ggctgcgcgg cgatggggga tcagcgacgg cgtacccgtg gtggtgtac
//
LOCUS       DATMSX010000403         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_110528,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000403 DATMSX010000000
VERSION     DATMSX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110528"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VN041_12685"
     CDS             <1..968
                     /locus_tag="VN041_12685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWU29929.1"
                     /translation="AERPVRSSVGVIAKPPIRKLARDLGVELTEVTPTGADGEVTRDD
                     VVKHAEQASVFRNIQTPEWGDVREETVPAPASAPAGLARGIRPDDRTESIPVKGVRKA
                     TSSAMVQSAYSAPHVTVWKEVDATRTMELVKRLKASPDFADIRVSPLLIMARAVIWAA
                     RRTPMVNAAWVDTADGAEISVRHYVNLGIAAATPRGLLVPNIKDAQDLGMKDLARALN
                     RLTLTAREGKTTPADQQGGTITITNIGVFGMDAGTPIINPGEAGIIAMGTIAQKPWVV
                     DGEVRPRWVTTVAGSFDHRVVDGDGMSRFVADVASILEEPALLVD"
     gene            1001..1576
                     /locus_tag="VN041_12690"
     CDS             1001..1576
                     /locus_tag="VN041_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HWU29930.1"
                     /translation="MDIPWAEGRWTHAPVSAVESGTDLLVTAQEGSDAWRTTSYGFVH
                     DTEHALLAPFAQDSAVEVEFTAAFSEQFDQAGVFIRAREDHWIKAGVEYADGRPQLGA
                     VVTDGMSDWSLAPVPDWLDRRILVRVSRTGDALTIRASVDGGELQLVRVVPFPPDAVA
                     QAGPMICAPTRAGLTVPFHAWRVTEPDGGLH"
     gene            complement(1622..>1816)
                     /locus_tag="VN041_12695"
     CDS             complement(1622..>1816)
                     /locus_tag="VN041_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29931.1"
                     /translation="LYVVVGACVVYATVRFAIVHALRQARGEERIERRLPNAATWLDE
                     DERRLLDVSNASQDGTSTGH"
BASE COUNT          274 a          662 c          631 g          249 t
ORIGIN      
        1 ctgccgagcg cccggtgcgc tcctccgtcg gcgtgatcgc caagccgccg atccgcaagc
       61 tcgcgcgcga cctcggcgtc gagctgaccg aggtcacgcc caccggcgcc gacggagagg
      121 tcacccgcga cgacgtcgtg aagcacgccg agcaggccag cgtcttccgc aacatccaga
      181 cgcccgaatg gggtgacgtg cgcgaggaga ccgtgcccgc cccggcatcc gctccggccg
      241 gcctcgcccg cggcatccgc cccgacgacc ggaccgaatc catcccggtc aagggcgtgc
      301 gcaaggcgac cagctcggcc atggtgcaga gcgcgtactc cgcgccgcac gtgacggtgt
      361 ggaaggaggt cgacgcgacc cgcacgatgg agctcgtcaa gcgcctgaag gcgtcgcccg
      421 acttcgccga catccgcgtc tcgccgctgc tgatcatggc ccgcgccgtg atctgggccg
      481 cgcgccgcac cccgatggtg aacgcggcct gggtcgacac ggccgacggc gccgagatct
      541 cggtgcgcca ctacgtgaac ctcggcatcg cggcggccac gccgcgcggt ctgctggtgc
      601 cgaacatcaa ggacgcgcag gacctgggca tgaaggatct cgcccgcgct ctgaaccgcc
      661 tgactctcac ggcccgtgag ggcaagacga ccccggccga tcagcagggc ggcaccatca
      721 cgatcacgaa catcggcgtg ttcggcatgg acgcgggcac cccgatcatc aaccccggcg
      781 aggccggcat catcgccatg ggcaccatcg cgcagaagcc gtgggtcgtc gacggcgagg
      841 tgcgtccgcg ctgggtgacc acggtcgccg ggtccttcga ccatcgcgtg gtcgacggcg
      901 acgggatgag ccgcttcgtc gccgacgtgg cctcgatcct cgaggagccc gcgctgctcg
      961 tcgactgatg aggcgggacg acacgatcgg aggagcagga atggacatcc cctgggctga
     1021 aggacgctgg acgcacgcgc cggtctccgc cgttgagagc ggcaccgacc tgctcgtcac
     1081 ggcgcaggag ggcagcgacg cctggcgcac gacctcgtac gggttcgtgc acgacaccga
     1141 gcatgcgctt ctggcgccgt tcgcgcagga cagcgcggtc gaggtcgagt tcacggcggc
     1201 gttctccgag cagttcgatc aggccggcgt cttcatccgt gcgcgcgagg accactggat
     1261 caaggccggc gtcgagtacg cggacggccg cccgcagctc ggtgccgtcg tcaccgacgg
     1321 catgtcggac tggtcgctcg ccccggtgcc cgactggctc gaccgtcgga tcctcgtccg
     1381 ggtgagccgc accggcgatg cgctcaccat ccgcgcgtcc gtcgacggcg gcgagctcca
     1441 gctggtgcgg gtcgtcccct tcccgccgga tgcggtggca caggcggggc cgatgatctg
     1501 cgcgcccacg cgcgccggcc tcacggtgcc cttccacgcc tggcgcgtca cagaaccgga
     1561 cggcgggctg cactagcgcg ccccggccac tcggcacccg gcgcctctcc ggatgtggaa
     1621 gtcagtggcc ggtgctcgtg ccgtcctggg aggcgttcga cacatcgagc agacgacgtt
     1681 cgtcctcgtc gagccaggtg gccgcgttcg gcaggcgacg ctcgatccgc tcctcgccgc
     1741 gggcctgtct gagtgcgtgg acgatggcga accgcacggt cgcgtaaacg acgcatgctc
     1801 cgaccaccac gtaaag
//
LOCUS       DATMSX010000404         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147746,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000404 DATMSX010000000
VERSION     DATMSX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147746"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VN041_12700"
     CDS             complement(<1..755)
                     /locus_tag="VN041_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU29932.1"
                     /translation="MQTTAEPQTRVTPAPAIRDAHQQDAVIELRGVRKGYGAGDRRVQ
                     AVDGVDLVIRRGEIVALLGPNGAGKTTTLDMLLGLTDADEGTAQVLGGRPSRATADGS
                     IAAVLQTGGLLSDLTVGETVRLIASLHGRAALGRVPEVLARTDLEKIARRRVSKCSGG
                     EQQRVKFALALVADPDVLVLDEPTAGMDVTARRNFWDVMRADADAGRTIVFATHYLEE
                     AEQFARRTVVMNHGRVVADAPTAQLRAGLGARTV"
     gene            complement(840..2708)
                     /locus_tag="VN041_12705"
     CDS             complement(840..2708)
                     /locus_tag="VN041_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU29933.1"
                     /translation="MQFHHHGYVSGEPRVLPAEGLGIDRPADLPDEMDVLIVGSGPAG
                     MLLAAQMSQFPTVSTRIIERRAGRLELGQADGIQPRSVETFQAFGFAERIIAEAYNIG
                     WMNFWGPDPENRENIIRTSRTEDYGLKISEFPHLIVNQARVLDYFAEAAAHGPGRIVP
                     DYGVEFVGLTVHDEGEYPVEVQVKHTAGPREGEERTIRAKYVAGCDGARSGVREAIGR
                     KHVGAMAAHAWGVMDVLVNTDFPDWRTKCAINAEAGNILHIPREGGYLSRMYIDLGAV
                     AEGDNRQVKHTPLEVVIQKANEILHPYTLDVKQVAWHSVYEVGHRVTDGFDDALDGDR
                     HPRVFLTGDACHTHSAKAGQGMNVSMQDGFNLGWKLGHVLSGRAPQELLSTYGAERRP
                     VAQQLIDFDKEWSSLMARKPEEISDPQELATFYLGTAEFPSGFMTQYEPSMIVDGASQ
                     QELAVGFPLGKRFKSAQVTRVSDGNAVHLGHHATADGRWRVYAFADQDATKLAAWADA
                     VEPVFARYTPRDGDVDAVFDVKAIYQQRHDEFEFTDAPALFRPQTGPFGLIDWEKVYS
                     AAPTVWCQDDIFEVRELSRDGVVVVVRPDQYVAAILPLDAVDRFSEFFEGALLPAS"
     gene            2856..>3271
                     /locus_tag="VN041_12710"
     CDS             2856..>3271
                     /locus_tag="VN041_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29934.1"
                     /translation="MPAAPASQTLSRGIRILEILADGPLSIDEVAARLDVHRSIAYRL
                     LRTLEDHRLVARNASGLAALGPRMAALAAGVAHDLQAEALPELTAVANEFALTAFLAV
                     LDGDEVITLTSVEPRHAVANVAQRPGTRHPVTAGAPG"
BASE COUNT          457 a         1127 c         1171 g          516 t
ORIGIN      
        1 accgtcctgg cgccgagccc ggcgcgcagc tgcgccgtgg gcgcgtccgc gaccactcgg
       61 ccgtggttca tgacgaccgt gcggcgggcg aactgctcgg cctcctccag gtagtgcgtg
      121 gcgaagacga tggtgcgccc ggcatcggca tccgcccgca tgacgtccca gaagttgcgg
      181 cgggcggtca cgtccatgcc ggcggtgggc tcgtcgagca cgagcacgtc ggggtcggcg
      241 accagggcca acgcgaactt cacgcgctgc tgctcgccac cggagcactt cgagacccgg
      301 cggcgcgcga tcttctcgag atcggttcgg gccagcacct cgggcacacg cccgagcgcg
      361 gccctgccgt gcagcgaggc gatcaggcgc acggtctcgc cgacggtgag gtcggagagc
      421 agcccgccgg tctgcagcac tgcggcgatg gagccgtcgg cggtggcgcg cgaggggcgg
      481 cctccgagca cctgcgcggt cccctcgtcg gcatccgtga gtccgagcag catgtcgagc
      541 gtggtcgtct tgcccgcacc gttcggcccg agcagcgcga cgatctcgcc gcggcggatg
      601 acgaggtcga ccccgtcgac ggcctgcacg cggcggtcgc ccgcgccgta gcccttgcgc
      661 acgccgcgca gttcgatcac ggcgtcctgc tgatgcgcgt cccggatggc gggagcgggg
      721 gtgacccgcg tctgcggttc tgctgtcgtc tgcatgcatc cagcgtcgtc ggcgcgggcg
      781 ccggacaccg gagggcgatg tcatgaggtc ggcatgacag atgtcatgcc gacccgacgt
      841 caggatgccg ggaggagtgc gccctcgaag aactcggaga accggtccac ggcatccagc
      901 ggcaggatcg ccgccacgta ctggtccggg cgcaccacga cgaccacgcc gtcgcgggag
      961 agctcgcgca cctcgaagat gtcgtcttgg caccacacgg tcggggccgc ggagtagacc
     1021 ttctcccagt cgatcagccc gaaggggccg gtctgcgggc ggaagagcgc cggcgcgtcg
     1081 gtgaactcga actcgtcgtg acgctgctgg tagatcgcct tcacgtcgaa cacggcgtcc
     1141 acgtcgccat ctcgcggcgt gtagcgcgcg aacacgggct ccacggcatc cgcccaggcc
     1201 gccagcttcg tggcgtcctg gtcggcgaag gcgtacacgc gccagcgccc gtcggccgtc
     1261 gcgtggtggc cgaggtgcac ggcgttgccg tcgctgaccc gggtgacctg ggccgacttg
     1321 aagcgcttgc cgagcgggaa cccgacggcg agctcctgtt gcgatgcgcc gtcgacgatc
     1381 atagacggct cgtactgcgt catgaacccg gaggggaact ccgccgtgcc caggtagaag
     1441 gtcgcgagct cctgcggatc ggagatctcc tcgggcttgc gggccatgag cgaggaccac
     1501 tccttgtcga agtcgatcag ctgctgggcg acgggacggc gctcggcgcc gtaggtcgag
     1561 agcagctcct gcggagcgcg tccgctgagc acgtgcccga gcttccagcc caggttgaag
     1621 ccgtcctgca tggacacgtt catgccctgg ccagccttcg cgctgtgcgt gtggcaggcg
     1681 tcgccggtca ggaagacgcg ggggtgccgg tcaccgtcca gcgcgtcgtc gaacccgtcg
     1741 gtgacccgat ggccgacctc gtagacgctg tgccaggcga cctgcttcac gtccagcgtg
     1801 taggggtgca ggatctcgtt ggctttctgg atgacgacct cgagcggcgt gtgcttgacc
     1861 tggcggttgt cgccctcggc gaccgcgccc aggtcgatgt acatccggct gaggtagccg
     1921 ccctcgcgcg ggatgtgcag gatgttgccc gcctcggcgt tgatcgcgca cttggtgcgc
     1981 cagtccggga agtcggtgtt gaccagcacg tccatcactc cccaggcgtg cgccgccatg
     2041 gcgccgacgt gcttgcggcc gatcgcctcg cgcaccccgc tgcgggcgcc gtcgcagccc
     2101 gcgacgtact tggcgcggat agtgcgctcc tcgccctcgc gggggccggc ggtgtgcttc
     2161 acctgcacct cgacggggta ctccccctcg tcatggaccg tgaggcccac gaactccacg
     2221 ccgtagtcgg ggacgatgcg tccggggccg tgggcggcgg cctcggcgaa gtagtccagc
     2281 acgcgcgcct ggttcacgat caggtgcggg aactcgctga tcttcaggcc gtagtcctcg
     2341 gtgcgcgagg tgcggatgat gttctcgcgg ttctcggggt cggggcccca gaagttcatc
     2401 cagccgatgt tgtacgcctc ggcgatgatc cgctcggcga acccgaatgc ctggaaggtc
     2461 tcgacgctgc ggggctggat gccgtcggcc tgacccagct ccagacgccc ggcgcggcgc
     2521 tcgatgatgc gcgtgctcac ggtcgggaac tgcgacatct gagcggccag cagcatgccc
     2581 gcgggaccgg agccgacgat gagcacgtcc atctcgtcgg ggagatcggc cgggcggtcg
     2641 atgcccagtc cttcggcggg gagtacgcgg ggctcgccgg atacgtagcc gtggtggtgg
     2701 aactgcatcg tcgggtcctt ccctggactg catcgtcagg tcttgcgcgg cgggaacgcg
     2761 tgttctatat tcgaacgcac cgttctacta ttgaacagat cgtatatgac gagcggatgc
     2821 cctgacaaga gccgcccgtg accgaaggag cacccgtgcc cgccgccccc gcctcgcaga
     2881 cgctgagccg cggcatccgg atcctggaga tcctcgccga cggccccctc tcgatcgacg
     2941 aggtcgccgc gcggctcgac gtgcatcgct cgatcgccta tcgactcctg cgcacgctcg
     3001 aggatcatcg gctcgtggcg cgcaacgcgt cggggctcgc ggcgctgggc ccacggatgg
     3061 cggcactggc cgcgggggtc gcacacgatc tgcaggccga ggcactcccc gagctcacgg
     3121 cggtcgccaa cgagttcgcc ctcaccgcgt tcctggcggt gctcgacggc gacgaggtca
     3181 tcacgctcac cagcgtcgag ccccgccatg cggtcgcgaa cgtggcgcag cggcctggca
     3241 cgcggcatcc ggtcacggcc ggggcgccgg g
//
LOCUS       DATMSX010000405         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_44513,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000405 DATMSX010000000
VERSION     DATMSX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44513"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VN041_12715"
     CDS             <1..518
                     /locus_tag="VN041_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWU29935.1"
                     /translation="VTAAPKASAGPATHIATPLVAVADPDDVVVIDNHGRTDVSCWGG
                     LLAEAAVQRGIAGVIVDGACRDVQESEALGLPVFARQAVPVSARGRIVQQAMDERIQV
                     AGVSVASHDYVIADVNGVVFVAAADVERVVELAGRIADRESRMAEAVRSGRDVSEVMH
                     DSQFPTVTASE"
     gene            523..1179
                     /locus_tag="VN041_12720"
     CDS             523..1179
                     /locus_tag="VN041_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HWU29936.1"
                     /translation="MSDDAIVQRFAALGTATVSDALDKHVRPGSLLGLAPLADGQHMA
                     GRAYTVRYISAQTPAGTVGDYIDQVQPGQVVVLDNDARVDCTVWGDILTAVAHHRGIA
                     GTVIEGVCRDTHRALSLGYPIYSHGRFMRTGKDRVEVAEEQGPVTIGGVTVRPGDILL
                     GDSDGVVVIPQDIEEDILETAETIHRREESILEAALNGATIAEARARFGYHNLQRAEG
                     "
     gene            1176..1829
                     /locus_tag="VN041_12725"
     CDS             1176..1829
                     /locus_tag="VN041_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWU29937.1"
                     /translation="MTSADALQDAEELLQLGSATLYEASRSDCFLDAAFRPAWEGAQI
                     VGRAYPVSAQLGDNLALHHGIEAAGDGDVLVVDAGGGVHGYWGEVMTVAAQARGIRGL
                     VIDGGVRDTAQLAGLGFPAFSTSISIRGTIKQWPGVLGRPVTLRGRVVHRGDVIVADR
                     DGIAVIPSARYDEVLASSRERAAKEDAFMARLRDGESTLDLYDFRGLGAPERGPAER"
     gene            1888..3555
                     /locus_tag="VN041_12730"
     CDS             1888..3555
                     /locus_tag="VN041_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU29938.1"
                     /translation="MTQAALDADVLIIGAGPVGLVAALDLSARGVSTIVVEQREFLQP
                     PNVKCNHVASRTMESFRRLGIAAEVRAAGLPADYPQDVAFRTSLTGIEFARIPIPASG
                     ERYTSRTGPDTAWQTPEPPHRINQTFLEPILARHAAQAPGVTLLNRTLFHSLAQDESS
                     VSAVISDLDGSNERTVRARYLIGADGGRSMVRKQIGATLSGDPVLQHVQSTCIRSAEL
                     YERMSADEPRAWGYYTFNQRRLGHVYAIDGRETFLVHTYLSPEEAQTEGSVDRDAAIR
                     AILGVGDDFEYEAVSKEDWIARRLLADRFRSGRVFIAGDASHLWVPFAGFGMNAGIAD
                     VLNLTWQLGAHLSGWADEGILDAYEAERRPITEQVSHFAMNHQQKLARPGMPAEIEDA
                     TEAGERARAEFGAAVHALNEPQFAAAGLNYGYVYDASPIISYDGAEAPGYTMGTYTPS
                     TVPGCRAPHFVLADGTSFYDRLSDGYTLVARDGHGSPALVAEAERRGIPLVVVEPGDR
                     LLPAEYTAPLTLVRQDQQVVWRGAALDAADAAELCELLRGARVRVVV"
     gene            complement(3632..>4881)
                     /locus_tag="VN041_12735"
     CDS             complement(3632..>4881)
                     /locus_tag="VN041_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU29939.1"
                     /translation="SAAAAQPAWAALGHAERSRLMRAIADDIDEHAEELGEIITHEQG
                     KVAGAGEAHGAAHFLRGAADTVLEPQRLDDRSELHYMPLGVVASIGPWNFPVMIGVWH
                     IAPALRMGNTVVIKPSEYTPLSVLALVEIFRRHLPEGVVAAVSGGREVGAALASHRDV
                     AKIVFTGSTPTGRRIVESSSNNLARLTLELGGNDAGIVLPGTDVSQVAEKLFWGAFMN
                     TGQVCAALKRLYVHDSVYDELVEALAGIAETAPIGSGHVKTNRLGPVQNPSQFDIVSR
                     LVDDARARGARIVTGGEPAAELGPLFYRPTIIADVEDGAAIVDEEQFGPVLPVVRYSD
                     VEDAVRRANGTDQGLGASVWGPDEEAAKEIALRLEAGTVWINQHGGVDPSTPFGGVKA
                     SGYGLEFGVEGLKAMAATKVIRR"
BASE COUNT          709 a         1804 c         1690 g          678 t
ORIGIN      
        1 cggtcaccgc ggcgcccaag gcatccgcgg gtccggccac ccacatcgcc acgccgctgg
       61 tcgccgtcgc cgaccccgac gacgtcgtcg tcatcgacaa tcacggccgc acggacgtct
      121 cctgctgggg cggtctgctc gccgaggccg ccgtccagcg cggcatcgcc ggcgtcatcg
      181 tcgacggcgc ctgccgcgac gtgcaggaga gcgaggcgct ggggctgccc gtgttcgccc
      241 gtcaggcggt gccggtcagc gcgcgcggcc ggatcgtgca gcaggcgatg gacgagcgca
      301 tccaggtcgc cggcgtctcg gtggcgtcgc acgactacgt gatcgccgac gtcaacggcg
      361 tcgtcttcgt cgcggcggcc gacgtggaac gcgtcgtcga gctcgccggc cgcatcgccg
      421 atcgggagtc gcggatggcc gaggccgtac gcagcggccg cgacgtgagc gaggtcatgc
      481 acgattccca gttccccacc gtcacagctt cggagtaatc gcatgtcaga cgacgccatc
      541 gtccagaggt tcgccgcgct cggcacggcg accgtgtcgg acgcgctcga caagcacgtc
      601 cgccccggca gcctgctcgg cctcgccccg ctcgccgacg gacagcacat ggcggggcgg
      661 gcctacacgg tccgctatat cagcgcgcag acgcccgcgg gcacggtcgg cgactacatc
      721 gaccaggtgc agcccggcca ggtggtcgtg ctcgacaacg acgcgcgcgt cgactgcacg
      781 gtctggggag acatcctgac cgccgtcgcg catcatcgcg gcatcgcggg cacggtcatc
      841 gagggcgtgt gccgcgacac ccaccgtgcg ctctcgctcg gctacccgat ctacagtcac
      901 ggccgcttca tgcgcaccgg caaggaccgc gtcgaggtcg ccgaggagca gggccccgtc
      961 acgatcggcg gtgtgaccgt gcgccccggc gacatcctgc tcggcgactc cgacggcgtc
     1021 gtcgtcatcc cccaggacat cgaggaggac atcctcgaga ccgcggagac catccatcgc
     1081 cgcgaggagt cgatcctcga ggcagcgctc aacggcgcca ccatcgccga ggcccgcgcg
     1141 cgcttcggct accacaacct gcagagggca gagggatgac ctccgccgac gctctgcagg
     1201 atgccgagga gctgctgcag ctcggcagcg ccacgctcta cgaggcgtcg cgcagcgact
     1261 gcttcctgga tgcggcgttc cgccccgcct gggagggcgc gcagatcgtc gggcgcgcgt
     1321 acccggtatc cgcccagctc ggtgacaacc tcgctctgca ccacggcatc gaggcggccg
     1381 gcgacggaga cgtgctcgtc gtggacgccg gcggcggggt ccacggctac tggggcgagg
     1441 tcatgacggt cgccgctcag gcgcgcggca tccggggtct cgtcatcgac ggcggcgtgc
     1501 gcgacaccgc acagctggcc ggcctgggct ttcccgcctt cagcacctcc atctccatcc
     1561 gcggcacgat caagcagtgg ccgggagtgc tgggccgtcc cgtcaccctg cgcggacgcg
     1621 tggtccaccg cggcgacgtc atcgtcgccg accgtgacgg catcgccgtg atcccctcgg
     1681 cgcgctacga cgaggtgctc gcatcctcgc gggagcgcgc cgccaaggag gacgccttca
     1741 tggccaggct gcgggacggc gagagcacgc tcgacctcta cgacttccgc gggctcgggg
     1801 cgccggagcg cgggccggcc gaacgctgat cgacgaccgc gcggccggat gcagacacct
     1861 cacactgatc ggaatcggga gaatcacatg acgcaggcgg cgctggacgc ggacgtcctc
     1921 atcatcgggg cgggccccgt cggactggtc gcagcgctcg acctcagcgc gcgaggggtg
     1981 tcgacgatcg tcgtggagca gcgcgagttc ctccagccgc cgaatgtgaa gtgcaaccac
     2041 gtcgcctccc gcaccatgga gagcttccgc cggctgggca tcgccgccga ggtgcgcgcg
     2101 gccgggctgc cggcggacta cccgcaggac gtggctttcc gcacctcgct gaccggcatc
     2161 gagttcgccc gcatcccgat ccccgccagc ggcgagcggt acacgtcgcg caccggaccg
     2221 gacaccgcgt ggcagacgcc cgagccgccg caccgcatca accagacctt cctcgagccg
     2281 atcctcgcgc gccacgccgc gcaggcaccc ggggtcacgc tgctgaaccg caccctcttc
     2341 cacagcctcg cgcaggacga gtcctcggtg tcggccgtga tctccgacct cgacggctcg
     2401 aacgagcgca cggtgcgcgc ccgctacctg atcggcgcgg acggcgggcg ctcgatggtg
     2461 cgcaagcaga tcggcgccac gctgagcggc gaccccgtgc tgcagcacgt gcagtccacc
     2521 tgcatccgct ccgccgagct gtatgagcgg atgtccgcgg acgagccgcg cgcctggggc
     2581 tactacacgt tcaatcagcg ccgcctgggc cacgtctacg cgatcgacgg gcgcgagacc
     2641 ttcctggtgc acacctacct ctcgcccgag gaggcacaga ccgaggggtc ggtggaccgg
     2701 gatgccgcga tccgcgcgat cctcggcgtc ggggacgact tcgagtacga ggccgtgtcg
     2761 aaggaggact ggatcgcgcg ccgactgctc gccgatcgct tccgcagcgg ccgcgtcttc
     2821 atcgccggcg acgccagtca cctctgggtg cccttcgccg gattcgggat gaacgccggc
     2881 atcgccgacg tgctcaacct cacctggcag ctgggcgcgc acctgagcgg atgggccgac
     2941 gagggcatcc tcgacgccta tgaggccgag cggcgcccga tcaccgagca ggtctcgcac
     3001 ttcgcgatga atcaccagca gaagctcgca cggcccggca tgcccgcgga gatcgaggat
     3061 gcgacggaag caggggagcg ggcccgcgcc gagttcggcg cggccgtgca cgccctcaac
     3121 gagccgcagt tcgccgcggc agggctcaac tacggctacg tgtacgacgc ctcgccgatc
     3181 atctcctacg acggagcgga ggcgcccggg tacacgatgg gcacctacac gccctcgacg
     3241 gtcccgggct gccgcgcccc gcacttcgtg ctcgccgacg gcacctcgtt ctacgaccgg
     3301 ctctccgacg gctacaccct ggtggcgcgc gacgggcacg gcagtcccgc cctcgtcgcg
     3361 gaggccgagc gacgcggcat cccgctggtc gtcgtggagc ccggagaccg gctgctgccc
     3421 gccgaataca cggccccgct cacgctggtc cgccaggatc agcaggtggt ctggcggggc
     3481 gccgcgctcg acgcggccga cgccgcagag ctctgcgaac tgctccgcgg cgctcgcgtg
     3541 cgcgtcgtcg tctgagcggc gccgctgcgg aacaggaagg cgccggatcg accgatccgg
     3601 cgcctttctc gttctcggga ggaggtcggg ctcagcggcg gatgaccttc gtcgccgcca
     3661 tcgccttcag cccctcgacg ccgaactcga gcccgtatcc ggaggccttc acaccgccga
     3721 acggggtcga cgggtcgacg cctccgtgct ggttgatcca cacggtgccg gcctcgagac
     3781 gcagtgcgat ctccttcgcc gcctcctcgt cgggccccca caccgatgcg ccgagcccct
     3841 ggtccgtgcc gttcgcgcgg cggacggcgt cctcgacatc gctgtagcgg accacgggca
     3901 ggacggggcc gaactgctcc tcgtcgacga tggcagcccc gtcctcgacg tcggcgatga
     3961 tcgtcggacg gtagaacagc gggccgagct cggccgcggg ctcgccgccg gtcacgatac
     4021 gcgcgccgcg tgccctggcg tcgtcgacca gccgtgagac gatgtcgaac tgcgaggggt
     4081 tctgcaccgg tcccagccgg ttggtcttca cgtgtccgga gccgatcggt gcggtctcgg
     4141 cgatgcccgc gagcgcctcg accagctcgt cgtacacgga gtcgtgcacg tacaggcgct
     4201 tgagcgccgc gcacacctgg ccggtgttca tgaatgctcc ccagaacagc ttctcggcga
     4261 cctgggagac gtcggtgcca gggagcacga tgccggcgtc gttgccgccg agctccaggg
     4321 tcaggcgggc gaggttgttc gacgagctct ccacgatgcg cctgcccgtc ggggtagacc
     4381 ccgtgaacac gatcttcgcg acatcgcggt gggaggcgag cgccgctccc acctcgcgcc
     4441 cgccggagac cgcggccacg accccctcgg gcaggtgccg gcggaagatc tccaccaggg
     4501 ccagcacgct cagcggcgtg tactccgagg gcttgatgac gaccgtgttc cccatccgca
     4561 gcgcgggcgc gatgtgccac acgccgatca tgacggggaa gttccacggg ccgatcgagg
     4621 cgaccacgcc gagcggcatg tagtgcagct cggaacgatc gtcgagccgc tgcggctcca
     4681 gcacggtgtc ggcggcgccg cgcaggaagt gggccgcgcc gtgcgcctct cccgcgccgg
     4741 ccaccttgcc ctgctcgtgc gtgatgatct cgccgagctc ctccgcgtgc tcatcgatgt
     4801 cgtcggcgat cgcacgcatc agacgggagc gctcggcatg gccgagggcg gcccacgcgg
     4861 gctgggcggc ggccgcagat c
//
LOCUS       DATMSX010000406         9498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147756,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000406 DATMSX010000000
VERSION     DATMSX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147756"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VN041_12740"
     CDS             complement(<1..283)
                     /locus_tag="VN041_12740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWU29940.1"
                     /translation="MGAQLRVYKQKISSAQTTKKITKAMELIAASRIQKAMARVRASS
                     PFARAVTQAVSAVATYSNVDHPLTREPETIRRSAVVVFSSDRGLAGAFNS"
     gene            complement(303..1943)
                     /gene="atpA"
                     /locus_tag="VN041_12745"
     CDS             complement(303..1943)
                     /gene="atpA"
                     /locus_tag="VN041_12745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWU29941.1"
                     /translation="MAELSISPDVIRDALKNFAAAYEPTGAVATEVGTVIDAADGIAH
                     VEGLPGVMANELVTFGDGTKGLALNLDEHEIGVVVLGDFTGIEAGQEVTRTGEVLSVP
                     VGDGYLGRVVDPLGNPIDGLGPVATDGVRALELQAPGVMQRKSVHEPMQTGVKAIDAM
                     IPVGRGQRQLIIGDRQTGKTALAIDTIINQKANWESGDVNKQVRCIYVAIGQKGSTIA
                     SVKGALEDAGALEYTTIVAAPASDPAGFKYLAPYTGSAIGQHWMYDGKHVLIVFDDLS
                     KQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSMTGLPIIETK
                     ANDVSAYIPTNVISITDGQIFLQSDLFNANQRPAVDVGISVSRVGGDAQVKSIKKVSG
                     TLKLELAQYRSLEAFAMFASDLDAASRRQLARGARLTELLKQPQYSPYPVEEQVVSIW
                     AGTNGKLDTLEVEDVLRFERDLLDYLRRNTSILDTLRETNVLDDATVAELDKQTDAFL
                     LEFQGGKGHAIGAPGHEEHAAAEADDVNQEKIVKGRRA"
     gene            complement(1994..2782)
                     /locus_tag="VN041_12750"
     CDS             complement(1994..2782)
                     /locus_tag="VN041_12750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HWU29942.1"
                     /translation="MGSATTQALAASTQALAAASGLTLDSAGELFAAARALGDSAQLS
                     GALADPTAPAQARSALVSSVFAGVSAPVRALLDTVAGERWSSASDLIDGVEELAIRAA
                     AIASAKDDVAGELFEFSRVVAANPELELALGSRLGDSAAKAALVEKVLGSSVSGATVL
                     IAASLVRLPRERRVRQLLSRAIDIVSAQAGRTVATVHTAAPLAAAQETRLRDALARRY
                     GGEISVNQVIDPTVVGGLRVQIADDVIDGSISARLAELRQKLAS"
     gene            complement(2782..3321)
                     /locus_tag="VN041_12755"
     CDS             complement(2782..3321)
                     /locus_tag="VN041_12755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWU29943.1"
                     /translation="MLNALVTNLAASEEPNPLIPAWYDIVWSALWFLIILIVVWKVAL
                     PKLTAMLDERSAAIEGNIAKADEAQKQAEAALDEYTRQLAEARTEAGQIRDAAREDGK
                     KIVAEAKDAASAEASRITTAAHTQIEAERQAAFVSLRNEVGTLAIDLAGGVVGETLTD
                     DARATAVVDRFLADLEKAK"
     gene            complement(3349..3585)
                     /gene="atpE"
                     /locus_tag="VN041_12760"
     CDS             complement(3349..3585)
                     /gene="atpE"
                     /locus_tag="VN041_12760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWU29944.1"
                     /translation="MDATTVLAAVNGNLAAVGYGLAAIGPAIGVGIVVGKTIEGVARQ
                     PELAGRLQVLMWIGIAFTEALAFVGIAVGFIPFP"
     gene            complement(3627..4415)
                     /gene="atpB"
                     /locus_tag="VN041_12765"
     CDS             complement(3627..4415)
                     /gene="atpB"
                     /locus_tag="VN041_12765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWU29945.1"
                     /translation="MILRPFAGDGEFHGPSMGDFFPPILFEVFGVPVHRIHLIQFLSV
                     IAVVLFMWLGTRRMKVVPGRFQSLVEMGIGFVRNGIAHDLLGRKDGERFAPLLVTIFF
                     MILFMNITGIIPFLNMPGTAIIAVPLTLAVISYVTFIYAGMKKSPLGFWKNSLFPSGV
                     PWPVYIIVTPLEFISTFIIRPVTLTLRLMMNLVVGHMILVLCFSATAFFMFTAGGGWA
                     ALGVGTLAFGGAFTIFEILVVVLQSYVFTVLTAIYIQLAVAEEH"
     gene            complement(4543..5055)
                     /locus_tag="VN041_12770"
     CDS             complement(4543..5055)
                     /locus_tag="VN041_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29946.1"
                     /translation="MTPSPVSSTPILRRTLILSAVAAGVLALIAAGIGFAVAGTDGLW
                     SGLIGVLLSFIFLGITAGSILFANRWFGDPLYVQFFFAIVLGGWLVKLGLFVVLMLVL
                     REQPWIDPLVFFLALVAGIAMTLVVDVIVLTKMRLPNVSDIDLPTTNPEDDGDDAQGA
                     PGAPKQTGDI"
     gene            complement(5052..6254)
                     /locus_tag="VN041_12775"
     CDS             complement(5052..6254)
                     /locus_tag="VN041_12775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWU29947.1"
                     /translation="MKQYLFTIILTASITFALTWAVWRLSMKFKLYPGIRERDVHKTP
                     KPRLGGVAMFIGIAVAILISTANPFFERMWSPPQTLWAILAAAALIAVVGVIDDLWDL
                     DWMIKLAAQFLAAGIITAWGGLQILSLPVGTMVLVSPWLSIAITMVAIVVVMNAVNFI
                     DGLDGLVAGVCLIANGIFFVYSYILTRDSGATSYFNVATFLAAVLIGACLGFLPFNWS
                     PAKIFMGDSGALVLGLLMATSAIAVTAQGDVNAFDPAKFGRSQLLGAFIPIVLPLVIV
                     MLPLLDFGLAVFRRMRAGKSPFAPDRKHLHHRMLDLGHRDRDAVLVFYAWTAVISLAV
                     LLMYIGTRQDWPGGYVGGIVFGVLGAAACLVVTRMPSRRSPRTDEPSEGEPQTLEATK
                     PTKPLEPR"
     gene            complement(6251..7042)
                     /locus_tag="VN041_12780"
     CDS             complement(6251..7042)
                     /locus_tag="VN041_12780"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWU29948.1"
                     /translation="MSTVFDCRDDAQILAGMRHARQAIARGELVVLPTDTVYGIAADA
                     FSPAAVQRLLDAKGRGREMPPPVLVAGPEMMAALVESVPDAVQKLIDAFWPGGLTIVL
                     PAQPSLTWDLGETKGTVAVRMPDRRVALELLAETGPLAVSSANLTGHDAAVIASDAQE
                     MLGDSVAVYLEEGYSENGIPSTIVDATSLVGTAEGETPAVRILRQGAVSREQLEEVLG
                     DLLEPAEKPDETVPGHVPDAAPPAVAAPAADAASAGDAGSPVDEE"
     gene            complement(7112..7969)
                     /gene="prmC"
                     /locus_tag="VN041_12785"
     CDS             complement(7112..7969)
                     /gene="prmC"
                     /locus_tag="VN041_12785"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWU29949.1"
                     /translation="MPDSLSARLRAVAQQLADAGVPDPLVDAELLIGHVLGLGRGELQ
                     AAVVRGDGLEDADAARLDALVSRRAAREPLQHLTGVAAFRHLELAVGPGVFVPRPETE
                     TVVQIAIDALMESASPSPIAIDLGTGSGAIALAMATEVPHARVFAVERSAEAHAWAAR
                     NTADAENLTLVLGDLATAFDELRGTASVVISNPPYVPDDAIPRDPEVRLHDPAQALYG
                     GADGLDVVRVISRRAQELLHPGGLLVLEHGELQGASIRELLEADGWRAAATHEDLTRR
                     DRATTALSR"
     gene            8081..9028
                     /gene="cysK"
                     /locus_tag="VN041_12790"
     CDS             8081..9028
                     /gene="cysK"
                     /locus_tag="VN041_12790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWU29950.1"
                     /translation="MTGIHSDITSAFGNTPLVRLNRVTEGLDATVLAKLEFYNPASSV
                     KDRIGIAMVDAAEASGELKPGGTILEATSGNTGIALAMVGAARGYKVILTMPASMSKE
                     RRVLLRAFGAEIVLTDPTKGMTGAIEALKEILAENPDAVWVRQFENEANPRIHRETTA
                     QEILRDTDGNIDILVAGVGTGGTVTGVGQALKAVKPEVQVIAVEPKDSPVLSEGHPGP
                     HKIQGIGPNFVPPVLDRDVIDEVYPVEFEDSLRTARDLAAKEGLLVGMSAGAAVWAAL
                     NIARRPENAGKTIVAVVPDTGERYISTALFEDLRDQPKD"
     gene            9030..>9498
                     /gene="epsC"
                     /locus_tag="VN041_12795"
     CDS             9030..>9498
                     /gene="epsC"
                     /locus_tag="VN041_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HWU29951.1"
                     /translation="MGMIGRLREDVAAARRRDPAARSGLEVALLYPGLHAIWAHRVWH
                     ALWRSGIRFPARMGSQVSRWLTGIEIHPGARIGRRFFIDHGMGVVIGETAEIGDDVML
                     YHGVTLGGRTRDHGKRHPTLEDGVAVGAGAKILGPVRIGARSVIGANAVVTRDA"
BASE COUNT         1586 a         3124 c         3374 g         1414 t
ORIGIN      
        1 gcgagttgaa ggcaccggcg agaccgcggt cggaggagaa gaccacgacg gcggaccgac
       61 ggatcgtctc cggctcacgc gtgagcgggt ggtcgacgtt ggagtaggtc gcgacggccg
      121 aaacggcctg cgtcacggcc cgcgcgaaag gcgaggatgc gcgaacacgc gccatcgcct
      181 tctgaatgcg cgaagccgcg atgagctcca tcgccttcgt gatcttcttg gtcgtctgag
      241 cagaactgat cttctgcttg tagaccctga gttgagcgcc catgaatcag tacctcacgc
      301 gattacgcgc gacgaccctt gacgatcttc tcctggttga cgtcgtccgc ctcggcggcg
      361 gcgtgctcct cgtggcccgg ggcgccgatg gcgtgaccct tgccgccctg gaactccaga
      421 aggaaggcgt cggtctgctt gtcgagctcg gcgacggtcg cgtcgtcgag gacgttcgtc
      481 tcgcgcaggg tgtcgaggat gctcgtgttg cgacgcaggt agtcgagcag gtcgcgctcg
      541 aagcgcagca cgtcctcgac ctcgagggtg tcgagcttgc cgttggtgcc ggcccagatc
      601 gagacgacct gctcctccac cgggtacggc gagtactgcg gctgcttgag cagctcggtc
      661 aggcgggcac cgcgtgccag ctgacgacgc gaggccgcgt cgaggtcgga tgcgaacatc
      721 gcgaacgcct cgagggagcg gtactgcgcg agctcgagct tcagcgttcc ggagaccttc
      781 ttgatcgact tgacctgggc gtcaccaccg actcgcgaga ccgagatgcc cacgtcgaca
      841 gcagggcgct ggttggcgtt gaacaggtcg gactgcagga agatctggcc gtcggtgatc
      901 gagatcacgt tggtcggaat gtacgccgag acgtcgttcg ccttggtctc gatgatcggc
      961 agaccagtca tcgagccggc gccgagctcg tcggacagct tcgcgcagcg ctccagcaga
     1021 cgggagtgca ggtagaacac gtcgcccggg taggcctcgc gccccggcgg gcggcgcagc
     1081 agcagcgaca cggcgcggta cgcctcggcc tgcttggaca ggtcgtcgaa gacgatcagc
     1141 acgtgcttgc cgtcgtacat ccagtgctgg ccgatggccg agccggtgta cggggcgagg
     1201 tacttgaagc ctgcggggtc ggaggcggga gcggccacga tggtggtgta ctccagcgca
     1261 ccggcgtcct cgagcgcacc cttcaccgag gcgatggtcg agcccttctg accgatcgcg
     1321 acgtagatgc agcgcacctg cttgttgacg tcgccggact cccagttggc cttctggttg
     1381 atgatcgtgt cgatcgccag cgccgtcttg ccggtctggc ggtcgccgat gatcagctgg
     1441 cgctggccgc ggccgacggg gatcatggcg tcaatcgcct tgacgccggt ctgcatcggc
     1501 tcgtgcaccg acttgcgctg catgacgccg ggcgcctgca gctcgagcgc gcgcacgcca
     1561 tccgtcgcga cggggccgag gccgtcgatc gggttgccga gcgggtcgac aacgcgaccc
     1621 aggtagccgt caccgaccgg gaccgagagg acctcgccgg tgcgggtcac ttcctggccg
     1681 gcctcgatgc cggtgaagtc gccgaggacg accacgccga tctcgtgctc gtcgaggttc
     1741 agcgcaaggc ccttggtgcc gtcaccgaag gtgacgagct cgttggccat gacgccgggc
     1801 agcccctcga cgtgcgcgat gccgtcggct gcgtcgatga cggtgccgac ctcggtcgcg
     1861 acggcgccag tgggctcgta ggcggctgcg aagttcttca gcgcgtcacg gatgacgtcg
     1921 gggctgatcg atagttctgc cattgtcttc cctttgtgtg tctggtgtgc gcggttcccc
     1981 gcgtcagtct gtgtcagctc gcgagcttct gacggagctc ggcgagtcgg gcggagatgc
     2041 tgccgtcgat gacgtcatcg gcgatctgca cgcgcaggcc gccgacgacg gtggggtcga
     2101 tcacctggtt cacggagatc tccccgccat agcggcgtgc cagagcgtca cggagacgcg
     2161 tctcctgcgc agcggcgagc ggtgcggccg tgtggacggt cgcgaccgtg cggccggcct
     2221 gtgcggagac gatgtcgatg gcccggctca gaagctgacg gacgcggcgc tcgcgcggca
     2281 ggcgcaccag cgaagcggcg atcagcacgg tcgcacccga aaccgaggag cccagcacct
     2341 tctcgacgag cgcggccttg gccgcggagt cgccgaggcg gctgccgagc gcgagctcga
     2401 gctccgggtt cgcggcgacg acgcgggaga actcgaagag ctcgccggcg acgtcgtcct
     2461 tcgcggaggc gatggccgca gcgcggatcg cgagctcctc gacaccgtcg atgagatcgg
     2521 atgcactgga ccagcgctca ccggcgacgg tgtccagcag agcgcgcacc ggagcggaca
     2581 ccccggcgaa cacactggag accagcgcgg agcgtgcctg ggcaggcgcc gtgggatcgg
     2641 ccagcgcgcc gctcagctga gccgaatccc cgagcgcgcg ggcggctgcg aacagctcgc
     2701 ccgcactgtc gagggtcagg cccgacgccg cggcaagcgc ctgcgtggat gccgcgagtg
     2761 cctgagtggt cgcgctgccc attacttcgc cttctcgagg tcggcgagga agcggtccac
     2821 gacggccgtc gcacgagcgt cgtcggtgag ggtctccccc accacgccgc cggcgaggtc
     2881 gatcgcgagc gtgccgacct cgttgcggag ggacacgaac gcggcctggc gctcagcctc
     2941 gatctgcgtg tgcgcggcgg tggtgatgcg gctcgcctca gcggacgcgg catccttcgc
     3001 ctcagcgacg atcttcttgc cgtcctcacg ggcggcgtcg cggatctgtc cggcctcggt
     3061 gcgcgcctcg gcgagctgac gggtgtactc gtcgagcgcg gcctcggcct gcttctgagc
     3121 ctcatcagcc ttggcgatgt tgccttcgat cgctgcggag cgctcatcga gcatcgccgt
     3181 gagcttcgga agggcgacct tccagaccac gatgaggatg atgaggaacc acagcgccga
     3241 ccagacgatg tcgtaccagg caggaatcag ggggttgggt tcctctgatg ctgccaggtt
     3301 cgtgacaaga gcgttcagca tcctgtctcc ttcagaagtc gctgcggttt acgggaaggg
     3361 gatgaatccg acggcgatgc cgacgaatgc aagcgcctcg gtgaaggcga taccgatcca
     3421 catcaggacc tgcaggcgac cggcgagctc gggctggcgg gcgacaccct cgatcgtctt
     3481 gccgacgacg atgcccacgc cgatggccgg gccgatggcg gcgaggccgt agccgactgc
     3541 cgcgaggttg ccgttgactg ctgcgagaac cgtagttgcg tccacggggt tttcctttcg
     3601 attttggtca gcagatgctg acccgttcag tgctcttctg cgaccgcgag ctggatgtag
     3661 atcgcggtga ggacggtgaa gacgtaggac tgcaggacga cgacgaggat ctcgaagatc
     3721 gtgaacgcgc cgccgaaggc gagggttccg acgccgagcg cggcccagcc gccgcctgcg
     3781 gtgaacatga agaatgcggt ggccgagaag cagagcacga ggatcatgtg cccgacgacc
     3841 aggttcatca tcagtcggag cgtgagcgtg accggacgga tgatgaaggt ggagatgaac
     3901 tccagcggcg tgacgatgat gtacaccggc cacggcacgc ccgaggggaa gagcgagttc
     3961 ttccagaacc cgagggggct cttcttcatg cccgcgtaga tgaacgtcac gtagctgatc
     4021 acggcgagcg tgagcgggac ggcgatgatc gcggttcccg gcatgttcag gaacgggatg
     4081 atgccggtga tgttcatgaa caggatcatg aagaagatcg tgaccagcag cggcgcgaac
     4141 cgctcgccgt ccttgcggcc gagcagatcg tgcgcgatgc cgttgcggac gaagccgatc
     4201 cccatctcga cgaggctctg gaagcggccg gggacgacct tcatccggcg cgtgccgagc
     4261 cacatgaaca gcacgacggc gatcaccgag aggaactgga tcaggtggat ccggtgaacc
     4321 ggaacaccga agacctcgaa caggatcggc gggaagaagt cgcccatcga gggaccatgg
     4381 aactcaccat caccggcgaa tggacgaagg atcattgtcg caacgggatt caacagcgcg
     4441 ggctccagct tcgggtcacc ggtcggaacc ggggcgacga gggacggtgt gcatcacagc
     4501 gcaagcgctc gcgaaacgag cggcgggcac ggttcaacac tatcagatgt cgccggtctg
     4561 ctttggcgca ccgggcgccc cctgggcgtc gtcaccgtcg tcttcggggt tcgtcgtcgg
     4621 gaggtcgatg tcgctgacgt tcgggagccg catcttcgtg aggacgatga catccacgac
     4681 gagagtcatc gcgatgcccg cgacgagagc gaggaagaag acgagcgggt cgatccatgg
     4741 ctgctcacgc agcaccagca tgagcaccac gaacagcccg agcttgacca gccagccgcc
     4801 gagcacgatc gcgaagaaga actgcacgta cagcgggtcc ccgaaccagc ggttcgcgaa
     4861 cagaatgctg cccgctgtga tcccgaggaa gatgaacgag aggagcacgc cgatcagccc
     4921 gctccacagc ccgtcggtgc cggcgaccgc gaagccgatg cccgcggcga tgagagccag
     4981 caccccggcc gcgacggcgg acaggatcag agtgcggcga aggatgggcg tgctggacac
     5041 ggggctcggg gtcatcgggg ctccaggggc ttcgtgggct tcgtggcttc aagggtctgc
     5101 ggttcacctt cggacggttc gtccgtgcgc gggctgcggc gcgagggcat ccgggtgacc
     5161 accaggcagg cggccgcgcc gagcacgccg aacacgatcc cgccgacgta gccgccgggc
     5221 cagtcctggc gcgtcccgat gtacatcagc agcaccgcga gcgagatcac ggccgtccag
     5281 gcgtagaaga cgagcacggc gtcgcggtcg cggtggccga ggtccagcat ccgatgatgc
     5341 aggtgcttgc ggtccggcgc gaacggcgac ttgcccgcgc gcatccgccg gaacacggcc
     5401 agaccgaagt ccagcagcgg cagcatcacg atgaccagcg gcaggacgat cgggatgaag
     5461 gcgcccagca gctgcgaacg gccgaacttc gcgggatcga acgcgttcac gtcgccctgg
     5521 gcggtgacgg cgatcgccga ggtggccatc agcagcccga gcacgagcgc gccggaatcg
     5581 cccatgaaga tcttggcggg gctccagttg aacggcagga aacccaggca ggcgccgatc
     5641 aggacggcgg cgaggaaggt ggccacgttg aagtagctgg tcgcgccgga gtcgcgggta
     5701 aggatgtagg agtagacgaa gaagatgccg ttcgcgatca ggcacacgcc cgcgacgagc
     5761 ccatcgagtc cgtcgatgaa gttcacggcg ttcatgacca cgacgatcgc caccatcgtg
     5821 atcgcgatgc tgagccacgg cgagacgagg accatggtcc cgacgggcag cgacaggatc
     5881 tgcagaccgc cccatgccgt gatgatgccg gcggcgagga actgcgcggc cagcttgatc
     5941 atccagtcca gatcccagag gtcgtcgatc acgccgacca cggcgatcag cgcggccgcg
     6001 gcgaggatcg cccagagcgt ctgcggcggc gaccacatcc gctcgaagaa cgggttcgct
     6061 gtggagatca ggatcgccac ggcgatgccg atgaacatgg ccacgccgcc cagccggggc
     6121 ttcggcgtct tgtgcacgtc ccgttcacgg atgccgggat agagcttgaa cttcatgctc
     6181 aagcgccaca ccgcccacgt cagcgcgaag gtgatcgacg ctgtgaggat gatcgtgaac
     6241 agatactgct tcactcttcg tctaccggac ttcccgcgtc ccctgccgat gcggcgtccg
     6301 cggcaggagc ggcgaccgcg ggaggagcgg catcgggaac atgacccggg accgtctcgt
     6361 cgggcttctc ggcgggctcg agcagatcgc cgaggacctc ctcgagctgc tcgcgggaga
     6421 ccgcgccctg gcgcaggatc cgcacggctg gggtctcccc ctccgcggtg cccaccagcg
     6481 aggtcgcgtc cacgatggtc gaggggatgc cgttctcgct gtagccctcc tccaggtaca
     6541 ccgcgacgga gtcgcccagc atctcctggg cgtcggaggc gatgaccgcg gcatcgtggc
     6601 cggtgaggtt cgcactggac accgccagcg ggccggtctc ggcgagcagc tcgagggcca
     6661 cgcggcggtc gggcatccgc accgcgaccg tgcccttggt ctcccccagg tcccaggtca
     6721 gcgacggctg cgcgggcagc acgatggtca gcccgccggg ccagaacgcg tcgatgagct
     6781 tctgcacggc atccggcacg gattccacca gagccgccat catctcgggc cccgcgacga
     6841 gcacgggcgg cggcatctcc cggccccggc ccttcgcatc gaggagccgc tggacggcgg
     6901 caggtgagaa cgcgtcggcg gcgatgccgt agacggtgtc ggtggggagg acgacgagct
     6961 cgccgcgggc gatcgcctga cgggcgtggc gcatgccggc gaggatctgg gcgtcgtcgc
     7021 ggcaatcgaa gacagttgac atggcgtgct cattctacgg tggtggcctg tcggtccgcc
     7081 gtggacggcg gacccgggtt tccggggagg ctcagcgact cagcgcggtg gtggcgcgat
     7141 cgcgacgggt cagatcctcg tgcgtcgccg ccgcccgcca cccgtcggcc tccagcagct
     7201 cccggatcga cgcgccctgc agctcgccgt gctccagcac gagaagtccg cccggatgca
     7261 gcagctcctg cgcccgcctg ctgatcaccc gcaccacgtc gagcccgtcc gcgccgccgt
     7321 agagcgcctg cgccggatcg tgcaggcgca cctccgggtc gcgcgggatc gcgtcgtccg
     7381 gtacgtaggg cgggttggag atcacgacgg atgccgtgcc gcgcagctca tcgaacgcgg
     7441 tggcgaggtc gccgagcacg agcgtcaggt tctcggcatc cgcggtgttc ctcgcggccc
     7501 aggcgtgcgc ctcggccgag cgctcgacgg cgaacacccg cgcgtgcggc acctcggtcg
     7561 ccatcgcgag agcgatcgcg ccgctgcccg tgcccagatc gatcgcgatc ggcgagggcg
     7621 aggcgctctc catcagtgcg tcgatcgcga tctgcaccac cgtctcggtc tccggcctgg
     7681 gtacgaagac gccaggcccc acggcgagtt ccaagtgccg gaaggcggcg acgccggtga
     7741 gatgctgcag gggttccctg gctgcgcgcc gcgagacgag ggcgtccagc cgagcggcat
     7801 ccgcgtcctc cagcccgtca ccgcggacga ccgcggcctg cagctcgccg cgtccgaggc
     7861 ccagaacatg gccgatcagc agctccgcat ccaccagcgg gtccggaacc ccggcgtcgg
     7921 cgagctgctg ggcgacggcg cgcagacgcg cggagaggga gtcgggcatc cctccagcgt
     7981 aggcgtcgaa cgcgctcgga cacattcggc gagggtcggg gatccgggtc gtaggctgag
     8041 cacgctgatc acttcgacac ccttcgacga aaggcccgcc atgaccggca tccactccga
     8101 catcacctcc gcattcggca acaccccgct ggtgcgcctg aatcgcgtca ccgagggcct
     8161 ggacgcgacc gtgctcgcga agctggagtt ctacaacccg gcctccagcg tcaaggaccg
     8221 catcggcatc gccatggtcg acgccgccga ggcgtcgggc gagctcaagc cgggcggcac
     8281 gatcctcgag gccaccagcg ggaacaccgg catcgcgctc gcgatggtgg gtgccgcgcg
     8341 cggttacaag gtgatcctga ccatgcccgc gtcgatgtcc aaggagcgtc gcgtgctgct
     8401 gcgcgcgttc ggcgccgaga tcgtgctcac cgacccgacc aagggcatga cgggagccat
     8461 cgaggcgctg aaggagatcc tcgccgagaa cccggatgcc gtgtgggtcc gccagttcga
     8521 gaacgaggcc aacccccgca tccaccgcga gaccaccgcg caggagatcc tgcgcgacac
     8581 ggacgggaac atcgacatcc tggtcgcagg tgtcggcacc ggtggcaccg tcaccggagt
     8641 cggccaggcg ctgaaggcgg tgaagcccga ggtgcaggtg atcgcggtcg agcccaagga
     8701 ctcccccgtg ctcagcgagg gccaccccgg tccgcacaag atccagggca tcggcccgaa
     8761 cttcgtcccg ccggtgctcg accgcgacgt gatcgacgag gtgtaccccg tggagttcga
     8821 ggactcgctg cgcaccgcgc gcgacctcgc cgccaaggag ggactgctcg tgggcatgtc
     8881 cgccggcgcc gcggtgtggg cggccctgaa catcgcccgc cgtcccgaga acgcgggcaa
     8941 gaccatcgtc gcggtcgtcc ccgacaccgg tgagcgctac atctcgaccg cgctgttcga
     9001 ggacctgcgc gaccagccga aggactgaga tgggcatgat cgggcggttg cgcgaggacg
     9061 tcgcagccgc acgcaggcgc gacccggctg cgcgctccgg actcgaggtg gccctgctgt
     9121 acccggggct gcacgccatc tgggcacacc gggtctggca cgcactgtgg cggagcggca
     9181 tccgcttccc ggcgcggatg ggttcgcagg tgtcgcgctg gctgaccggc atagagatcc
     9241 accctggtgc gcgcatcggc cgccgcttct tcatcgacca cggcatggga gtcgtgatcg
     9301 gcgagaccgc ggagatcggc gacgacgtga tgctctacca cggcgtgacg ctgggcggcc
     9361 gcactcgcga ccacggcaag cggcatccga cgctggagga cggcgtcgca gtcggcgccg
     9421 gagccaagat cctcggtccg gtgcgcatcg gcgcgcgctc ggtcatcggg gcgaacgcgg
     9481 tcgtgacccg tgacgccc
//
LOCUS       DATMSX010000407         7348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_120125,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000407 DATMSX010000000
VERSION     DATMSX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120125"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..753
                     /gene="recR"
                     /locus_tag="VN041_12800"
     CDS             160..753
                     /gene="recR"
                     /locus_tag="VN041_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWU29952.1"
                     /translation="MYDGIVQELIDEFGRLPGIGPKSAQRIAFHILQTPSFDVARLSE
                     LLTEVRARVRFCEVCGNVSEQDRCTICRDPRRNAELICVVEDAKDVAAIERTREFRGL
                     YHVLGGAISPIAGVGPDDLRIAQLMTRLADGTVQEVILATNPNLEGEATASYLSRLLT
                     SMEITVSRLASGLPVGGDLEYADEVTLGRAFEGRRRL"
     gene            756..1673
                     /locus_tag="VN041_12805"
     CDS             756..1673
                     /locus_tag="VN041_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU29953.1"
                     /translation="MQAGFTRKGWILFAVMALVWGITYLFIKEAVETFGPPAVVAGRT
                     LLGGLVLLPFAIRSGALKAARKHWPWVLVFGLLEMGGPFLLLSHAETQLPSGLTGLLV
                     ATVPLFAVIIALLRGDRSALAPIRLGGLLVGFAGVVVVVAGPGLFPHEPASMIAIGEI
                     LLTAVMYAIAPFVIAYKLSDVPSIGTITLALFAIGLAYLPAALLTPHEPPTVRSAGSL
                     LLLGVICTALAFVAFFALIREVGPVRAPLFTYINPIVAIVLGTVLVAEPITPGLLIGF
                     PIILIGCWFAATGGRLKPRASEVAPAAIE"
     gene            1760..3046
                     /locus_tag="VN041_12810"
     CDS             1760..3046
                     /locus_tag="VN041_12810"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWU29954.1"
                     /translation="MALIVQKYGGSSVADAESIKRVAKRIVDTRRAGHDVVVAVSAMG
                     DTTDEMLDLAHEVAPIPAPRELDMLLSSGERISMALLAMAIHSMGFEARSFTGSQAGM
                     ITDSHHGKARIVDVTPVRLREALDEGAIVIVAGFQGFNRDTRDITTLGRGGSDTTAVA
                     LAAALKADVCEIYSDVDGIYTADPRVIPRAAKLDHISSEEMLELAANGAKVLYIRAVE
                     YARRHRVLIHARSTFSSAEGTYVLGEGMKNPRESEGAAMEEPIVAGVATDLSQAKITV
                     AGVPDVPGKAAEIFKIVSKSGANVDMIVQNVSAASTGRTDISFTLSKADAAGTIKALA
                     AEQSAVGFENLLHDDQIGKLSVVGAGMRTHSGVSEILFDALSRGGVNIEMISTSEIRI
                     SVVVRGSDLAQAAQAVHTAYGLDSEIEAVVHAGTGR"
     gene            3192..3674
                     /locus_tag="VN041_12815"
     CDS             3192..3674
                     /locus_tag="VN041_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU29955.1"
                     /translation="MTTDAGAKTESDAGALTIRDLEPADRDAWAALFRGYRDFYELEH
                     DDAVIDTVWEWLRDPGHELNALLAVRDGAPIGIAHWRRFARPSRGATAIFLDDLFVSP
                     EVRGGGVGRALLTRLHDIAREQGLIEVRWITSYTNTDAQRLYDRVALRAPFHTYIDQV
                     "
     gene            3916..4989
                     /locus_tag="VN041_12820"
     CDS             3916..4989
                     /locus_tag="VN041_12820"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWU29956.1"
                     /translation="MTRISDSGFSIAIVGATGQVGTVMREILAERSFPIRELRLFASA
                     RSAGTSIDFGGTGVIVEDVETADASGIDIALFSAGATASRAYAPKFAAAGAVVVDNSS
                     AWRMDPEVPLVVSEVNPDAMDERPKGIIANPNCTTMAAMPVMKALHAEAGLERLIVST
                     YQAVSGSGLAGAQELLGQVEGVLAQGDTLRLVHDGSSVDFPQPEKYVAPIAFDVIPLA
                     GSIVDDGDNETDEEKKLRNESRKILGLPDLRVAGTCVRVPVFTGHSLSIHAEFANDIT
                     PERAYEVLAAAPGVVVDEVPTPLQAAGKDPSFVGRIRADQSAPEGKGLVLFISNDNLR
                     KGAALNAVQIAEELAKRLVPASA"
     gene            5037..6497
                     /gene="mqo"
                     /locus_tag="VN041_12825"
     CDS             5037..6497
                     /gene="mqo"
                     /locus_tag="VN041_12825"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HWU29957.1"
                     /translation="MTETVDVVLIGGGIMSATLGTLLHELQPDWKIVAYERLSDVAEE
                     SSHAFNNAGTGHAALCELNYTPEAADGSVDVSKAVSINEQFQLSRQFWSSLVDRGVLS
                     EPDTFINPTPHMTFVRGEKNVSFLKTRYEALSKQTLFADMEYSEDSRVINKWAPLLME
                     KRLTADAPFAATRVTGGTDVDFGALTHQLFDHLKSSGVQVLPNHEVRSLKKQKDGTWL
                     VKNRQTVGRTPSQVKARFVFVGAGGWALKLLQNSGIPEIKGYGCFPIGGQWLMTTKPE
                     IVSKHQAKVYSKATVGAPPMSVPHLDTRVVDGETSVLFGPFATFSPKFLKNGSNWDIV
                     TQVRPSNLFSMLKVAVTNFDLIKYLVTELLKNHSRKVDDLRELMPDAKDEDWTLRQAG
                     QRAQVMHNGNLQFGTEVITSADGSISGLLGASPGASTAVPIMLGLLKRCFAEEYAGWE
                     PRLRELIPTLGEKLNDQPELAEKTFAETSATLSLQS"
     gene            6508..7137
                     /locus_tag="VN041_12830"
     CDS             6508..7137
                     /locus_tag="VN041_12830"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWU29958.1"
                     /translation="MAKLYFRYGAMNSGKSTALLQAAYNYEERGQRVLLAKPEIDTKG
                     ASQIESRLGMTREVDFLIGSDDDARALFAVHRQRIRDEADEELLPAAPADVACLLIDE
                     AQFLTPEQIDDLFRIAVLDGIPVLAYGIRNDFRTHAFPGSARLLAIAHTLEELKTICR
                     CGRKAVFNGRKVDGRFVFDGDQVAIDGVAVTYESLCGNCYLEESGGVLG"
     gene            7197..>7348
                     /locus_tag="VN041_12835"
     CDS             7197..>7348
                     /locus_tag="VN041_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU29959.1"
                     /translation="MPETTARAWRTVLARVESDLLDGVLGPGDRLPSERDLAADLGVG
                     RSSVREA"
BASE COUNT         1154 a         2618 c         2438 g         1138 t
ORIGIN      
        1 gccgtctgca gccgagtccc gtgcgtccgc gccggtcgtc tcccgggtcg cgcccgtcgc
       61 cggcggcgtc gagcgacggg gagaggccgt gatccgccag gtgctcggag cccggttcct
      121 gcgtgaagag ccctaccagc ccccgacgag gttcaactga tgtacgacgg catcgtccag
      181 gagctgatcg acgagttcgg ccggctcccc ggcatcggcc cgaagtccgc gcagcgcatc
      241 gcgttccaca tcctgcagac cccgtccttc gacgtcgcgc gcctgtcgga gctgctcacc
      301 gaggtgcgcg cccgcgtgcg gttctgcgag gtgtgcggca acgtgtccga gcaggatcgc
      361 tgtacgatct gccgcgatcc gcgccgcaac gccgagctga tctgcgtggt cgaggacgcc
      421 aaggacgtcg cggccatcga gcgcacacgc gagttccgcg gtctgtacca cgtgctcggc
      481 ggcgcgatca gccccatcgc gggcgtcggg cccgacgacc tgcgcatcgc ccagctgatg
      541 acccgtctcg ccgacggcac cgtgcaggag gtcatcctcg ccacgaaccc caacctcgag
      601 ggcgaggcca cggccagcta cctcagccgg ctgctcacca gcatggagat cacggtctcg
      661 cggctggcat ccggcctgcc cgtcggcggc gatctggagt atgcggacga ggtgacgctg
      721 ggacgggcct tcgagggccg tcgccgcctg tgaacgtgca ggcgggattc acccggaaag
      781 gctggatcct gttcgccgtc atggcgctcg tgtggggcat cacgtacctg ttcatcaagg
      841 aggcggtcga gaccttcggg ccgccggcgg tggtcgccgg acgcacgctg ctgggtggac
      901 tcgtgctgct gccgttcgcg atccgcagcg gcgcactgaa agcggcccgg aagcactggc
      961 cgtgggtgct cgtattcggt ctgctcgaga tgggcgggcc cttcctgctc ctcagccatg
     1021 ccgagacgca gctgccttcg gggctcaccg ggctcctggt ggcgacggtg ccgctgttcg
     1081 cggtgatcat cgccctgctg cgcggcgaca ggtccgcgct cgcgccgatc cggctgggcg
     1141 gtctgctcgt cgggttcgcc ggcgtggtgg tggtcgtcgc cgggcccggc ctgttcccgc
     1201 acgagccggc cagcatgata gcgatcggcg agatcctgct caccgccgtg atgtacgcga
     1261 tcgcgccgtt cgtcatcgcg tacaagctct ccgacgtccc ctcgatcggc acgatcacgc
     1321 tggcgctgtt cgcgatcggg ctggcgtatc tcccggcggc gctgctgacc ccgcacgagc
     1381 cgccgaccgt tcgctccgcg ggatcattgc tgctcctcgg agtgatctgc acggcgctcg
     1441 cgttcgtcgc cttcttcgcg ctgatccgcg aggtcgggcc ggtgcgtgcc ccgctgttca
     1501 cctacatcaa cccgatcgtc gcgatcgtgc tcggcacggt cctcgtcgcg gaaccgatca
     1561 cccccggtct gctgatcggc ttcccgatca tcctgatcgg atgctggttc gccgccaccg
     1621 gcgggcggct gaagccccgg gcatccgagg tggcgcctgc ggccatcgag tagcggacgc
     1681 cgtcacatgc gcgggacggc atggagccga tccgtacaat gatcctgggc ggttccgccc
     1741 accgtccccg ggagtgatcg tggccctgat cgtgcagaag tacggcggtt cgtccgtcgc
     1801 cgacgcagag agcatcaagc gcgtcgccaa gcgcatcgtc gacacccgtc gcgccggcca
     1861 cgacgtcgtc gtcgccgtga gcgccatggg cgacaccacc gacgagatgc tggatctcgc
     1921 gcacgaggtg gcgccgatcc ccgccccgcg cgaactggac atgctgctct ccagcggtga
     1981 gcgcatctcg atggcgctcc tggcgatggc catccactcg atgggcttcg aggcgcgctc
     2041 gttcaccggc agccaggccg gcatgatcac ggactcgcac cacggcaagg cgcgcatcgt
     2101 cgacgtcacc ccggtgcgcc tgcgcgaggc gctggacgag ggggcgatcg tcatcgtcgc
     2161 cggcttccag ggcttcaacc gcgacacccg cgacatcacc acgctcggcc gaggcggctc
     2221 cgacaccacc gccgtggctc tggcggctgc gctgaaggcc gatgtctgcg agatctacag
     2281 cgacgtcgac ggcatctaca cggcggaccc gcgcgtcatc ccgcgcgccg ccaagctcga
     2341 tcacatctcc agcgaggaga tgctcgagct cgccgcgaac ggcgcgaagg tgctgtacat
     2401 ccgcgccgtc gaatacgcgc gccgtcatcg cgtgctcatc cacgcacgat ccacgttctc
     2461 gtccgccgag ggcacctacg ttctgggcga gggcatgaag aacccccgcg aatccgaggg
     2521 agcagccatg gaagaaccca tcgtcgccgg cgtcgccacc gacctcagcc aggcgaagat
     2581 cactgtcgcg ggcgtgccgg acgttcccgg taaggccgct gagatcttca agatcgtctc
     2641 caagtcgggc gccaacgtcg acatgatcgt gcagaacgtg tcggccgcct ccacgggccg
     2701 caccgacatc tccttcacgc tgtccaaggc cgacgccgcc ggcaccatca aggcgctcgc
     2761 cgccgagcag tccgcggtcg gcttcgagaa tctgctgcac gacgaccaga tcggcaagct
     2821 ctcggtcgtc ggcgcgggca tgcgcacgca ctcgggcgtc tccgagatcc tcttcgacgc
     2881 gctcagccgc ggcggcgtga acatcgagat gatctccacc tccgagatcc gcatctcggt
     2941 cgtggtgcgc ggatccgacc tggctcaggc ggcgcaggcc gtgcacaccg cctacggcct
     3001 ggacagcgag atcgaggccg tcgtccacgc cggtaccggc cgctgacgcc tcccgacgcc
     3061 gtccgcgccc gggacacctg cacgagcggg gaacgcctgc acggccggag ccccggattc
     3121 gccgtgcatc cgttcccgga ccgtgcaccc gtgcccgacg tgcaccggtg ccgacccgat
     3181 acgatcgtcg gatgacgacg gatgccgggg cgaagaccga atcggacgca ggagctctca
     3241 cgatccgtga cctcgagccg gcggatcgcg acgcctgggc cgcgctgttc cgcggctacc
     3301 gcgacttcta cgagctcgag cacgatgacg ccgtcatcga cacggtctgg gagtggctgc
     3361 gggatcccgg ccacgagctg aacgcgctgc tggcggtgcg cgacggcgcc ccgatcggca
     3421 tcgcgcactg gcgtcgtttc gcccgcccgt cgcgcggcgc gacggccatc ttcctggacg
     3481 acctcttcgt ctcgcccgag gtgcgcggcg ggggagtggg ccgcgccctg ctgacccggc
     3541 tgcacgatat cgcccgcgag caggggctga tcgaggtgcg ctggatcacc tcatacacca
     3601 acaccgacgc ccagcgcctg tacgaccggg tcgccctccg cgcgccgttc cacacgtaca
     3661 tcgaccaggt ctgacggccg cttcggtaca gatgtcagtt cagagtacgg atgtcggccg
     3721 agatccgtac tttcggctga cattcgtact ttcggctgac aatccgtacg ggctggcacg
     3781 ccgacgcccg cgtcacggca gatccccgac ccccgatcag cgggtagact caccgcaacc
     3841 ctgacatcgg cgccgggcgc agcatcctca cccggggctg agcctggcgc accgtctcca
     3901 cgccaaggac tgctcatgac ccgcatctca gactcaggtt tctcgatcgc catcgtcggc
     3961 gcgaccggac aggtcggcac cgtcatgcgc gagatcctcg ccgagcgctc gttcccgatc
     4021 cgcgagctgc ggctgttcgc gtccgcccgc tcggccggga cgagtatcga cttcggcggc
     4081 accggcgtga tcgtcgagga tgtggagacc gcggatgcct cgggcatcga catcgcgctg
     4141 ttctcggcgg gtgcgaccgc cagccgcgcc tacgcgccga agttcgccgc cgccggcgcc
     4201 gtcgtggtcg acaactccag cgcgtggcgg atggacccgg aggtcccgct ggtcgtcagc
     4261 gaggtcaacc cggatgccat ggacgagcgt cccaagggga tcatcgccaa cccgaactgc
     4321 accaccatgg ccgcgatgcc cgtcatgaag gcgctgcacg cggaggccgg tctcgagcga
     4381 ctgatcgtca gcacctacca ggccgtctcc ggctccggcc tcgccggtgc ccaggagctg
     4441 ctcggtcagg tggagggcgt gctcgcccag ggcgacaccc tgcggctcgt gcacgacggc
     4501 tcctccgtcg acttcccgca gccggagaag tacgtggcac cgatcgcgtt cgacgtcatc
     4561 ccgctggccg gctccatcgt cgacgacggt gacaacgaga ccgacgagga gaagaagctc
     4621 cgcaacgaga gccgcaagat cctcgggctc cccgacctgc gcgtcgccgg cacctgcgtg
     4681 cgcgtgcccg tgttcaccgg ccactcgctg tcgatccacg cggagttcgc gaacgacatc
     4741 accccggagc gcgcctacga ggtcctcgcc gcagccccgg gcgtcgtggt cgacgaggtg
     4801 ccgaccccgc tccaggcggc aggcaaggac cccagcttcg tcgggcgcat ccgcgccgac
     4861 cagtccgcgc ccgagggcaa gggactggtg ctgttcatca gcaacgacaa cctgcgcaag
     4921 ggtgcggcgc tgaacgccgt gcagatcgct gaggagctcg cgaagcgcct ggtcccggca
     4981 tccgcctgag ccgtcgcgcg gaaatcggga cggatgccga cggctagact tatcgggtga
     5041 ctgaaaccgt cgatgtcgtt ctgatcggtg ggggcatcat gtccgccact ctgggtaccc
     5101 tgctgcacga actgcagccg gactggaaga tcgtcgcata tgagcgactc agcgacgtcg
     5161 ccgaggagag ctcgcacgcg ttcaacaacg cgggcaccgg ccatgccgcg ctctgcgagc
     5221 tgaactacac gccggaggcg gccgacggca gcgtcgacgt cagcaaggcg gtctcgatca
     5281 acgagcagtt ccagctcagc cggcagttct ggtcgagcct ggtcgaccgc ggtgtgctca
     5341 gcgagcccga cacgttcatc aacccgactc cgcacatgac cttcgtgcgc ggcgagaaga
     5401 acgtctcctt cctgaagacg cgctacgagg cgctgtcgaa gcagacgctg ttcgccgaca
     5461 tggagtacag cgaggactcc cgcgtcatca acaagtgggc gccgctgctc atggagaagc
     5521 gactcaccgc ggacgcgccc ttcgccgcga cgcgcgtcac cggcggcacc gacgtcgact
     5581 tcggcgccct gacccaccag ctcttcgacc acctgaagag ctccggcgtg caggttctcc
     5641 cgaaccacga ggtgcgctcg ctcaagaagc agaaggacgg cacgtggctg gtgaagaacc
     5701 gccagacggt cggccgcacc cccagccagg tcaaggcgcg cttcgtgttc gtcggcgccg
     5761 gcggctgggc cctcaagctt ctgcagaaca gcggcatccc cgagatcaag ggctacggat
     5821 gcttcccgat cggcggccag tggctgatga ccaccaagcc cgagatcgtc tccaagcacc
     5881 aggccaaggt gtactccaag gcgaccgtcg gcgccccgcc gatgtccgtc ccgcacctgg
     5941 acacgcgtgt cgtcgacggc gagacctcgg tgctgttcgg gccgttcgcc acgttcagcc
     6001 cgaagttcct gaagaacggc tcgaactggg acatcgtcac ccaggtgcgt ccctcgaacc
     6061 tgttcagcat gctgaaggtc gctgtcacca acttcgacct catcaagtac ctggtgaccg
     6121 agctgctcaa gaaccacagc cgcaaggtcg acgacctgcg tgagctcatg ccggatgcca
     6181 aggacgagga ctggacgctg cgtcaggccg gtcagcgcgc ccaggtcatg cacaacggca
     6241 acctgcagtt cggcaccgag gtcatcacct cggcggacgg ctcgatctcg ggtctgctgg
     6301 gcgcatcgcc gggcgcgtcc acggccgtgc cgatcatgct gggcctgctc aagcgctgct
     6361 tcgccgagga gtacgcgggt tgggagcccc ggctgcgcga gctgatcccc acgctgggag
     6421 agaagctgaa cgaccagccc gagctcgcag agaagacgtt cgcggagacc tcggcgaccc
     6481 tgtcgctgca gagctgacgg acccaccgtg gcgaagctgt acttccgcta cggcgcgatg
     6541 aactcgggca agtcgacggc tctgctgcag gcggcctaca actacgagga gcgcgggcag
     6601 cgggtgctgc tcgccaagcc cgagatcgac accaagggcg cctcgcagat cgagagccgc
     6661 ctcggcatga cccgtgaggt cgacttcctg atcggctccg atgacgacgc ccgtgcgctg
     6721 ttcgcggtgc accggcagcg catccgcgac gaggccgacg aggagctgct ccccgcggcg
     6781 cccgccgacg tggcgtgcct gctgatcgac gaggcgcagt tcctcacgcc cgagcagatc
     6841 gacgatctgt tccggatcgc ggtgctcgac ggcatccccg tgctcgccta cggcatccgc
     6901 aacgacttcc gcacgcatgc gttcccgggc tctgcccggc tgctggcgat cgcccacacc
     6961 ctggaggagc tgaagacgat ctgccgctgc ggccgcaagg ccgtcttcaa cgggcgcaag
     7021 gtcgacggcc ggttcgtgtt cgacggtgac caggtcgcga tcgacggcgt cgccgtgacg
     7081 tacgagtcgc tgtgcggcaa ctgctacctg gaggagtccg ggggagtgct gggctgacgg
     7141 ccgtttgcgt ggtctgacca catcggataa cctgtggtct gaccacagga ggacggatgc
     7201 cggagaccac tgcgcgcgcc tggcgcaccg tgctcgcacg ggtggagagc gatctgctcg
     7261 acggcgtgct cggcccgggg gaccggctgc cgtctgagcg cgacctcgcc gccgacctcg
     7321 gcgtggggcg ctcgagcgtc cgcgaggc
//
LOCUS       DATMSX010000408         8088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_24936,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000408 DATMSX010000000
VERSION     DATMSX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24936"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /gene="rpsA"
                     /locus_tag="VN041_12840"
     CDS             <1..1447
                     /gene="rpsA"
                     /locus_tag="VN041_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWU29960.1"
                     /translation="TTAPATKQVAINDIGSAEDFLAAVEKTLKFFNDGDIIEGTIVKI
                     DRDEVLLDVGYKTEGVIPSRELSIKHDVDPNEVVAVGDKVEALVLQKEDKEGRLILSK
                     KRAQYERAWGDVEKIKENDGVVTGTVIEVVKGGLIVDIGLRGFLPASLIELRRVRDLT
                     PYLGQELEAKILELDKNRNNVVLSRRALLEQTQSESRTTFLNNLHKGQVRKGVVSSIV
                     NFGAFVDLGGVDGLVHVSELSWKHIEHASEVVEVGQEVTVEILEVDLDRERVSLSLKA
                     TQEDPWQVFARTHAIGQIAPGKVTKLVPFGAFVRVADGIEGLVHISELSSKHVELAEQ
                     VVSVGEEVFVKVIDIDLERRRISLSLKQANESVDPHGTEFDPALYGMLAEYDENGEYK
                     YPEGFDAETGAWKDGFDAQREAWEQEYAAAQTRWEAHKAQVLKAAEAEAAAGDDFGAA
                     QSFSSDSAGAGTLADDEALAALREKLSGGNA"
     gene            1564..2163
                     /gene="coaE"
                     /locus_tag="VN041_12845"
     CDS             1564..2163
                     /gene="coaE"
                     /locus_tag="VN041_12845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWU29961.1"
                     /translation="MPLIALTGGIASGKSTIAARLAEQGAVIVDADRIVRDVQAPGSP
                     VLDELAAAFGDDVIAEDGSLNRAALGAKVFGDRTQLDRLNAIVHPAVRDESARRFAAA
                     FAADPDAVVVYDVPLLVEARADDPWDLIVVADAPADLRARRLVELRGLTEDEARARLA
                     SQVSDDDRRAVADVVIDTSVSLDRTRAQTDALWERVRPR"
     gene            2160..3374
                     /locus_tag="VN041_12850"
     CDS             2160..3374
                     /locus_tag="VN041_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU29962.1"
                     /translation="MRFTGHVPGTREYRRLIAGLFFAGVATFAQLYAPQAVLPQLSHA
                     LDITPATAAVTVSAGTLGIAVAVIPWSIVADRIGRVPAMTVGVVIATAVGLIAPLSGD
                     VSMLLTLRLVEGIALGAVPAVALAYLSEEVHARHVATAAGSYIAGTTVGGLAGRIVSG
                     LVGESLDWRSGVWAAVGMCAASAVLFVWLTPRAQGFIAGRGRADRGPAAGERLRAALT
                     SAPLWALYAQGFLLMGAFVAVYNYLGFHLSAPPFALPAWLVTLLFLAYLAGTASSPWA
                     GLLASRNGRLPVLLCSIGVMAIGTALMTLPATAGVLVGLVLFTAGFFGAHAVASGWTP
                     VAAGADARAQASSLYYFAYYAGSSLFGWGLGVLFGGAGWEALIIAVLAMCALAAGAAA
                     LALRPGALTSGS"
     gene            3562..4110
                     /locus_tag="VN041_12855"
     CDS             3562..4110
                     /locus_tag="VN041_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29963.1"
                     /translation="METSTSQISRRTVVKGAAWSVPVIAAAVAAPMAVASGDGNTVTN
                     SDANYYWASESQSTWSSLDPAQGNLEVRYSTRIAFKQEPWTSGPPAGGVLQVTVVFSQ
                     EVTLKAPIGQNWVQSSPAGLGPARTFVFQLSPSGFGGDISINLNGTTPGAITASSTMS
                     LINGGTTTWASESSDATAVLVS"
     gene            complement(4119..4715)
                     /locus_tag="VN041_12860"
     CDS             complement(4119..4715)
                     /locus_tag="VN041_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HWU29964.1"
                     /translation="MIEFLIGSSLAASAGLNAWMPLFLLGLADRLIPAVQLPAGWSWL
                     SGDIALWVIGALLVLEIVADKIPALDSLNDIVQSVLRPASGGIAFGAGASAQTVAVED
                     PSAFFTDNAWVPIVIGVGIALAVHVVKAVGRVAANSVTGGLAAPALSTAEDGASFMLA
                     VAAIVVPILALVLLVGLIVAAGVMTKRRRARKRAAAVG"
     gene            4798..6867
                     /gene="uvrB"
                     /locus_tag="VN041_12865"
     CDS             4798..6867
                     /gene="uvrB"
                     /locus_tag="VN041_12865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWU29965.1"
                     /translation="MQPTRSVRPFEVVSEYMPAGDQPQAIAELAARINAGETDIVLLG
                     ATGTGKSATTAWLIEQVQRPTLVMAHNKTLAAQLANEFRELMPNNAVEYFVSYYDYYQ
                     PEAYVPQTDTFIEKDSSVNAEVERLRHSTTNSLLSRRDVVVVSTVSCIYGLGAPEEYL
                     RAMVALQVGERYDRDALIRQFISMQYNRNDVDFSRGNFRVRGDTIEIIPVYEESAIRI
                     ELFGDEIEALYSLHPLTGEVIEKLDSVPIFPASHYVAGTDVVQRAIGTIEAELADRLA
                     ELERQGKLLEAQRLRMRTTFDLEMLQQLGFCSGIENYSRHLDGRAAGEPPHTLLDFFP
                     DDFLLVIDESHVTVPQIGAMYEGDASRKRTLVEHGFRLPSAMDNRPLRWDEFKNRIGQ
                     TVYLSATPGKYEMGIADGVVEQIIRPTGLIDPEIIVKPSKGQIDDLLEEIRIRVEQDE
                     RVLVTTLTKKMAEELTDFLGEHGVRVRYLHSDVDTLRRVELLTELRAGVYDVLVGINL
                     LREGLDLPEVSLVAILDADKEGFLRSGTSLIQTIGRAARNVSGQVHMYADNMTDSMAK
                     AIEETERRREKQIAYNKANGIDPQPLRKRIADITEVLAREAADTADMRAGRNKSGKGK
                     SPTPNLRRTGIAAEGATQLEDTITDLSEQMLAAAAELKFELAARLRDEVQDLKKELRA
                     MERAGHA"
     gene            complement(6888..7130)
                     /locus_tag="VN041_12870"
     CDS             complement(6888..7130)
                     /locus_tag="VN041_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29966.1"
                     /translation="MTEERDRPPLRTRIREAGGFYAWFNSRLIRLAGPAAVGPYESTP
                     PPSAAERAERACPLCGKPMNLHEIDRSGPKPLMHCP"
     gene            7175..7612
                     /locus_tag="VN041_12875"
     CDS             7175..7612
                     /locus_tag="VN041_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU29967.1"
                     /translation="MTDDDDLLRLENQLCFAMVTAARNVLAVYRPILEPLGLTHPQYL
                     VMLALWERSPRSLGDIADALALEPASASPLVKRLEKEGLIERVRSEQDERRLDISLAD
                     AGRALRAQALDVPRQVMQRVGLDLEAVSALREALGPFNRSLRG"
     gene            7663..>8088
                     /locus_tag="VN041_12880"
     CDS             7663..>8088
                     /locus_tag="VN041_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29968.1"
                     /translation="MTGAVQHDAGERTESVQRAPGARRVQPWRRMLLVFGALALFAVA
                     MLASAIAGHPTIDLTPEGATASPLPEERPTPTITPGAPPAQEGGGSDAALVGIGIVLA
                     LIVSALAVLLLLWIVRRIIDLWRSRRLRRQPGADTDVEVG"
BASE COUNT         1275 a         2753 c         2831 g         1229 t
ORIGIN      
        1 aacgaccgcc ccggccacca agcaggtcgc gatcaacgac atcggatctg ccgaggactt
       61 cctggccgcg gtcgagaaga ccctgaagtt cttcaacgac ggcgacatca tcgagggcac
      121 gatcgtcaag atcgaccgcg atgaggttct gctcgacgtc ggatacaaga ccgagggtgt
      181 catcccctcg cgtgagctct ccatcaagca cgacgtcgac cccaacgagg tcgtcgccgt
      241 cggcgacaag gtcgaggccc tcgttctcca gaaggaggac aaggaaggcc gtctgatcct
      301 ctccaagaag cgtgcgcagt acgagcgcgc ctggggcgat gtcgagaaga tcaaggagaa
      361 cgacggcgtc gtcaccggca ccgtcatcga ggtcgtcaag ggcggcctca tcgtcgacat
      421 cggactgcgc ggcttcctgc cggcgtcgct gatcgagctg cgtcgcgtcc gcgacctcac
      481 cccgtacctc ggccaggagc tcgaggcgaa gatcctcgag ctcgacaaga accgcaacaa
      541 cgtggttctg agccgtcgcg cgctgctgga gcagacgcag tcggagtcgc gcaccacgtt
      601 cctgaacaac ctgcacaagg gtcaggtccg caagggtgtc gtgtcctcga tcgtcaactt
      661 cggtgcgttc gtcgacctgg gcggcgtgga cggcctcgtg cacgtctccg agctgtcctg
      721 gaagcacatc gagcacgcct ccgaggtcgt cgaggtgggc caggaggtca ccgtcgagat
      781 cctcgaggtg gacctggacc gcgagcgcgt ctccctgtcg ctgaaggcga cgcaggagga
      841 cccgtggcag gtcttcgccc gcacccacgc gatcggtcag atcgcaccgg gcaaggtcac
      901 caagctcgtc ccgttcggtg ccttcgtgcg cgtcgcggac ggcatcgagg gcctcgtgca
      961 catctccgag ctctccagca agcacgtcga gctggctgag caggtcgtgt cggtgggcga
     1021 agaggtcttc gtcaaggtca tcgacatcga cctcgagcgt cgccgcatct cgctgtcgct
     1081 gaagcaggcc aacgagtccg tcgacccgca cggcaccgag ttcgacccgg cgctgtacgg
     1141 catgctcgcc gagtacgacg agaacggcga gtacaagtac ccggagggct tcgacgccga
     1201 gaccggtgcc tggaaggacg gcttcgacgc tcagcgcgag gcttgggagc aggagtacgc
     1261 ggctgcccag acccgctggg aggcgcacaa ggctcaggtc ctcaaggccg ccgaggccga
     1321 ggccgccgca ggcgacgact tcggtgcggc tcagtcgttc tcgagcgact ccgccggtgc
     1381 cggcaccctc gccgacgacg aggctctcgc ggctctgcgc gagaagctct cgggtggcaa
     1441 cgcgtaagca ccgcttcaac ggccatctcg gtcaacggca acgggccgtc acctcgcctt
     1501 cgggcgggtg gcggcccgtt ccgcgtccgc ggtgcgggtc cgagggcatg ccaggataag
     1561 aggatgccac tcatcgctct caccggcgga atcgcgtcgg ggaagtcgac catcgcagcc
     1621 cggctcgccg agcaaggcgc cgtcatcgtg gatgccgatc gcatcgtgcg cgatgtgcag
     1681 gcgccgggat cgccggtgct ggacgaactg gcggcggcat tcggcgatga tgtgatcgcc
     1741 gaggacgggt ccctgaaccg cgccgcactg ggcgcgaagg tcttcggcga ccggacgcag
     1801 ctcgaccggc tcaacgccat cgtgcatccc gccgtgcgag acgagtccgc gcggcggttc
     1861 gcggctgcct tcgcggcgga ccccgatgcg gtggtcgtct acgacgtccc gctcctggtc
     1921 gaggcccgtg cggacgaccc ctgggacctg atcgtggtcg cggatgctcc ggcagacctg
     1981 cgcgcacgcc gactggtcga gctgcggggc ctgaccgagg acgaggcgcg ggcgcgtctg
     2041 gcatcccagg tctccgatga cgaccgacgg gccgtcgccg acgtcgtgat cgacacctcg
     2101 gtctcgctgg accgcacccg cgcgcagacc gacgcgctgt gggagcgggt ccggccacgg
     2161 tgaggttcac gggccacgtc ccgggcacgc gcgagtaccg gcggctgatc gccggactgt
     2221 tcttcgcggg cgtcgcgacg ttcgcgcagc tctacgcgcc gcaagccgtg ctcccgcagc
     2281 tgtcgcatgc gctcgacatc acgccggcga ccgccgcggt gacggtgtct gccggcacgc
     2341 tgggcatcgc cgtcgcggtg atcccgtggt cgatcgtggc ggaccgcata ggcagggtcc
     2401 cggcgatgac cgtcggtgtc gtcatcgcca cggcggtagg cctgatcgcg cccctcagcg
     2461 gcgacgtctc gatgcttctg acactgcgcc tggtcgaggg gatcgcactc ggcgccgtgc
     2521 cggctgtggc gctggcctac ctcagcgagg aggtccacgc gcgacacgtc gcgaccgcag
     2581 ccggcagcta catcgcgggc acgaccgtcg gcgggctcgc cggccgcatc gtgtccgggc
     2641 tggtggggga gtcgctcgac tggcggagcg gcgtgtgggc cgcggtcggg atgtgcgcgg
     2701 catccgcggt cctgttcgtg tggctgacgc cgcgcgcaca gggcttcatc gcgggacgcg
     2761 gtcgagccga ccgcggaccg gcggcggggg agcgcctgcg cgcggcgctg acctcggcgc
     2821 cgctgtgggc gctgtacgcc caggggttcc tgctgatggg cgcgttcgtc gcggtgtaca
     2881 actatctcgg cttccatctg tcggcaccgc cgttcgccct gcctgcgtgg ctggtgacgc
     2941 tgctgttcct cgcatatctg gcgggcacgg cctcgtcgcc ctgggccggg ctgctggcat
     3001 cccgcaacgg caggctcccc gtgctgctct gctccatcgg agtgatggcg atcggcacgg
     3061 cgctcatgac gctgccggcg acggccggcg tactcgtcgg gctggtcctg ttcacggccg
     3121 gcttcttcgg cgcgcacgcg gtggcttcgg gctggacccc ggtcgcggcc ggtgcggatg
     3181 ccagggcgca ggcctcctcg ctctactact tcgcgtacta cgccggctcg agcctgttcg
     3241 gctgggggct gggggtgctg ttcggaggtg cgggctggga ggcgctgatc atcgcggtgc
     3301 tggcgatgtg cgcgctggcg gccggggcgg cggcgctcgc cctgcgccct ggggcgctca
     3361 cctccggttc gtaagcactg cttacatact ggtatcctgg tggtggggaa gtgacaacac
     3421 actggggatt ccgctgtgcg gtatcaactg cagttctttc cgggtgcgcg aagcagcgcc
     3481 cgtgacgcag gtgctgacgc cgtgacgcga cgggcgctcg tgcgtgcctt catgcacatc
     3541 acgagaacag gaagaccaca catggagacc tccacgtcgc agatctcgcg ccggaccgtc
     3601 gtcaaggggg cggcctggtc cgttcccgtc atcgcagccg cggtcgcggc cccgatggcg
     3661 gtggccagcg gtgacgggaa caccgtgacg aattcggatg ccaactacta ctgggcgtcg
     3721 gagtcgcaga gcacctggtc ctcgctcgat cccgcgcagg ggaatctcga ggtccgctac
     3781 tcgacccgga tcgccttcaa gcaggagccg tggacatcgg gaccgcccgc gggcggcgta
     3841 ctgcaggtca ccgtcgtgtt ctcgcaggag gtgacgctca aggctccgat cggacagaac
     3901 tgggttcagt cgtcgccggc gggcctcggc ccggcgagga ccttcgtctt ccagctgagt
     3961 ccgtcgggct tcggcggcga catcagcatc aacctcaacg ggacgacgcc cggtgccatc
     4021 actgcgagca gcacgatgag cctcatcaac ggcggcacga ccacctgggc gagcgagtcc
     4081 agcgacgcca ccgcggtcct cgtgagctga ggtccgactc agccgacagc ggcggcgcgc
     4141 ttgcgagcgc gtcgccgctt cgtcatcacc ccggctgcga cgatcaggcc gaccagcaga
     4201 acgagcgcca ggatcggcac cacgatcgcg gccaccgcga gcatgaagga cgccccgtcc
     4261 tccgctgtgc tgagggcggg tgcggcgagg ccaccggtca cggagttcgc agccacgcgt
     4321 ccgacggcct tgacgacgtg cacggcaagg gcgatgccca ctccgatcac gatcggcacc
     4381 caggcgttgt ccgtgaagaa cgcggagggg tcctcgaccg cgaccgtctg ggcgctggca
     4441 cccgctccga acgcgatgcc gccggacgcc ggtcgcagca cgctctgcac gatgtcgttg
     4501 agggagtcga gcgcggggat cttgtcagcg acgatctcga gcacgagcag ggcgccgatc
     4561 acccacagcg cgatgtcgcc gctgagccac gaccatccgg ccggcagctg aacggcgggg
     4621 atcagacggt cggcgagacc gagcaggaac agcggcatcc aggcgttgag gccggcggat
     4681 gccgccaggc tggatccgat caggaactcg atcacggctc cactctaggt ggccgcgggc
     4741 gcgggcgagg agcgcgcccc ggcccgagtg tcggtgaccc ctcgtaggct ggagagcatg
     4801 caacccacgc gcagcgtccg ccctttcgag gtcgtcagcg agtacatgcc cgcaggcgac
     4861 cagccgcagg ccatcgccga gctcgcggcg cgcatcaacg cgggtgagac cgacatcgtg
     4921 ctgctcggag ccacgggaac cggcaagtcc gcgacgacgg cgtggctcat cgagcaggtg
     4981 cagcggccca ccctggtgat ggcgcacaac aagaccctgg ccgcgcagct ggccaacgag
     5041 ttccgcgagc tcatgccgaa caacgccgtc gagtacttcg tcagctacta cgactactac
     5101 cagcccgagg cgtacgtgcc gcagacggac accttcatcg agaaggactc ctcggtgaac
     5161 gccgaggtgg agcggctccg gcactccaca acgaactcgc tgctcagccg gcgcgacgtc
     5221 gtcgtcgtca gcacggtctc ctgcatctac ggcctcggcg ccccggagga gtacctgcgc
     5281 gccatggtgg ccctgcaggt gggcgagcgc tacgaccgcg acgccctcat ccggcagttc
     5341 atctccatgc agtacaaccg caacgacgtg gacttctcgc gcggcaactt ccgcgtgcgc
     5401 ggcgacacga tcgagatcat ccctgtgtac gaggagagcg cgatccgcat cgagctcttc
     5461 ggagacgaga tcgaggcgct gtactccctg catccgctca ccggcgaggt gatcgagaag
     5521 ctcgactcgg tgccgatctt cccggcatcg cactacgtcg ccggcacgga tgtcgtgcag
     5581 cgtgcgatcg gcacgatcga ggccgagctc gccgatcggc tggccgagct ggaacggcag
     5641 ggcaagctgc tcgaggcgca gcggctgcgc atgcgcacga cgttcgacct cgagatgctg
     5701 cagcagctcg gcttctgctc cggcatcgag aactactccc gtcatctcga cgggcgcgcg
     5761 gcgggggagc cgccgcacac cctgctcgac ttcttccccg acgacttcct gctggtgatc
     5821 gacgagtcgc acgtgaccgt gccgcagatc ggcgcgatgt acgaaggcga cgcctcgcgg
     5881 aagcgcacgc tggtcgagca cgggttccgg ctgcccagcg cgatggacaa ccgtccgctg
     5941 cgctgggacg agttcaagaa ccgcatcggc cagaccgtgt acctctccgc gacgccgggc
     6001 aagtacgaga tgggcatcgc cgacggcgtc gtggagcaga tcatccgccc gaccggtctg
     6061 atcgacccgg agatcatcgt caagccctcg aaggggcaga tcgacgacct gctcgaggag
     6121 atccgcatcc gggtcgagca ggacgagcgc gtgctggtga ccacgctcac caagaagatg
     6181 gccgaggagc tgaccgactt cctcggcgag cacggcgtgc gcgtgcggta cctgcactcc
     6241 gacgtggaca cgctgcgccg tgtcgagctg ctcaccgagc tgcgtgccgg cgtgtacgac
     6301 gtcctcgtcg gcatcaacct gctccgcgag ggtctcgacc ttcccgaggt gtccctggtc
     6361 gcgatcctgg atgccgacaa ggagggcttc ctgcgctcgg ggacatcgct gatccagacc
     6421 atcggccggg cggcgcgaaa cgtgtcgggt caggtgcaca tgtatgccga caacatgacc
     6481 gactcgatgg cgaaggcgat cgaggagacc gagcggcgcc gcgagaagca gatcgcctac
     6541 aacaaggcca acggcatcga tccgcagcct ctgcgcaagc gcatcgccga catcacggag
     6601 gtgctggcgc gcgaggcggc cgacacggcc gacatgcgcg cagggcggaa caagtccggc
     6661 aagggcaagt cgcccacacc gaacctgcgt cgcacgggga tcgccgcgga gggcgccacg
     6721 cagctcgagg acacgatcac cgacctgtcg gagcagatgc tcgcagcggc cgccgaactg
     6781 aagttcgagc tcgccgcacg cctgcgcgac gaagtgcagg atctgaagaa ggagctgcgg
     6841 gccatggaga gggccggaca cgcctgacct cgacggcgct gctcgattca ggggcagtgc
     6901 atgagcggct tgggccccga ccggtcgatc tcgtgcaggt tcatcggctt cccgcacagc
     6961 gggcaggcgc gttccgcgcg ctctgccgcg ctcggtggcg gcgtgctctc gtaggggccg
     7021 acggccgccg gacccgccag gcggatcagc cgggagttga accatgcgta gaagccgccg
     7081 gcttcgcgga tgcgggtgcg aagcggaggg cggtctcgtt cctcggtcat gatgattagt
     7141 gtactaacta ttcgtgcacg agtagcatcg aggcgtgacc gatgatgacg acctcctgcg
     7201 cctcgagaac cagctgtgct tcgcgatggt cacggctgcc cgcaacgtcc tggccgtgta
     7261 ccggccgatt ctcgaaccgc tcggcctcac gcatccgcag taccttgtga tgctcgcgct
     7321 ctgggagcgg tctccgcgct ccctcggcga catcgccgac gcgctcgcgc tcgagccggc
     7381 gagcgcatcc ccgctcgtga agcggctcga gaaggagggg ctgatcgaac gggtgcgcag
     7441 cgagcaggac gaacggcgcc tcgacatcag cctggcggat gccggccgcg cgctgcgcgc
     7501 gcaggcgctg gacgtgccgc ggcaggtgat gcagcgcgtc gggctcgatc tggaggcggt
     7561 gagtgcgctc cgcgaggcgc tcggtccgtt caacaggtcg ttgcgcggct gagctcctcg
     7621 catccgcgcg gctgcacctg ccgactcgca tagggtgaag gtgtgacggg ggcggtgcag
     7681 cacgacgcgg gggagcggac ggagtccgtc cagcgtgcgc ccggtgcgag gcgggtccag
     7741 ccctggcggc ggatgctgct ggtcttcggc gcgctcgcgc tcttcgccgt cgccatgctc
     7801 gcctcggcca tcgccggaca tcccacgatc gacctcacgc cggaaggggc caccgcgtcg
     7861 ccccttcccg aggagcggcc gacgcccacg atcacgcctg gcgcgccgcc ggcccaggag
     7921 ggcggcggat cggatgccgc cctggtcggc atcggcatcg tcctcgcgct gatcgtctcg
     7981 gcgctggccg tcctcctgct gctctggatc gtccgtcgca tcatcgacct ctggcgcagc
     8041 cgccggctgc gcaggcaacc gggcgcggac acggacgtcg aggtcgga
//
LOCUS       DATMSX010000409         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_15509,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000409 DATMSX010000000
VERSION     DATMSX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15509"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VN041_12885"
     CDS             complement(<1..290)
                     /locus_tag="VN041_12885"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWU29969.1"
                     /translation="MKFSGELPAVDLTYSDVFLVPRRSAVSSRLQVDLAPHDGTPATI
                     PLVSSNMNSVTGPRLAAALARRGGIGVLPQDLPLQELDAAIRWVKRQPVRWDT"
     gene            454..4122
                     /locus_tag="VN041_12890"
     CDS             454..4122
                     /locus_tag="VN041_12890"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HWU29970.1"
                     /translation="MSNQVTGVGGDGGFGANSWLVDELYEQFKQDRNSVDKEWWPILE
                     NYTPDAAAAQTARTSDAPSVAPVTAPIPVIGAQPVARTTTKPAKQAPIPAQAPKPQAK
                     EETESAEEDKVTPLRGMPKTLAANMDQSLTVPTATSVRTIPAKLMIDNRIVINNHMSR
                     TRGGKVSFTHLIGWALIKTLKEFPSQNVFYAEIDGKPSVVAPAHVNLGIAVDVPKPDG
                     TRALLVPSIKRADTMTFSEYLLAYEDLIARARNNKLTAADFAGTTVSLTNPGGIGTVH
                     SVPRLMKGQGAIIGAGALNYPAEFQGSSEKVLTELGIGKVITLTSTYDHRVIQGAGSG
                     EFLKKVHELLIGERGFYDDIFAALRIPYQPIRWNADIAVDLAERVDKQARVQELINSY
                     RVRGHLMADIDPLEYVQRSHPDLEIESHGLTFWDLDREFVTGGFGGKRVMKLREILGV
                     LRDSYCRTLGIEYMHIQDPEQRRWFQEKVEVKYVKPGHDEQLRVLSKLNEAEAFETFL
                     QTKFVGQKRFSLEGGESLIPLLDEILQGAANAGLDGAAIGMAHRGRLNVLTNIAGKTY
                     GQVFREFEGSLTPGNQRGSGDVKYHLGTEGTFVAEDGAELPVYLAANPSHLETVDGVL
                     EGIARAKQDRKPIGTFTWLPILVHGDAAFAGQGVVVETLQMSQLRGYRTGGTVHIVVN
                     NQVGFTTLPTDSRTSVYATDVAKTIQAPIFHVNGDDPESVIHVAQLAFEYRERFHRDV
                     VIDLVCYRRRGHNEGDDPSMTQPLMTDLIQAKRSVRKLYTEALVGRGDITEEEYDKAK
                     ADFQNRLEIAFAETHAAETGATPIAPELTQVDPQVGEPEVTGVPLEVIHLIGDAHANK
                     PDGFTVHPKLQQQLDKRVEMSRTGGIDWGFGELLAFGSLLVEGTPVRLAGQDSRRGTF
                     VQRHAVLHDRENGQEWLPLTNLSASQGRFFVYDSLLSEYAALGFEYGYSVENTEALVL
                     WEAQFGDFVNGAQSVIDEYISAAEQKWGQQSGVVLLLPHGYEGQGPDHSSARMERFLQ
                     LCAQDNMTVARPSTPASYFHLLRRQAYTRPRRPLIVFTPKAMLRLRGATSQVEDFTQG
                     RFEPVIDDDRALDRAAVKRVVVHAGKIHWDLASELEKNPNPEIALVRLEQFYPTPIDA
                     LKAITDSYPNAELVWAQDEPENQGAWPFLALAFAEVPGPRQFRVVSRPAAASPSTGSS
                     KVHAAEQAALLKQALTLD"
BASE COUNT          741 a         1513 c         1392 g          593 t
ORIGIN      
        1 gtgtcccagc gcaccggctg gcgcttgacc cagcggatcg cggcatccag ctcctgcaac
       61 ggcagatcct ggggcagcac gccgatcccg ccgcgccggg cgagcgcggc ggcgagccgc
      121 ggcccggtca ccgagttcat gttcgacgac accagcggga tggtggcggg cgtgccgtcg
      181 tgcggcgcca gatccacctg gagccggctg gagacggcgg atcgccgtgg caccaggaag
      241 acgtccgaat aggtcagatc cactgcgggc agctcaccgg agaacttcat atcgtcacgc
      301 tacccctcca cgcctggagc gaaccgcctg accggggagc atcccgatgg cgattggcat
      361 taggctggga gactgttaca gacgtgcgac ccgacgcgct ctcgccgccg agccgcatgc
      421 acagcttcag cgatgaaagt agggcggtag agcgtgtcga accaggtgac cggcgtcggg
      481 ggcgacgggg ggttcggagc caattcgtgg ctcgtggacg agctgtacga gcagttcaag
      541 caggatcgca actccgtcga caaggagtgg tggccgattc tggagaacta cactccggat
      601 gccgccgcgg cgcagaccgc gcgcacgtcg gacgcgccga gcgtcgcgcc ggtgacggca
      661 ccgatccccg tgatcggtgc acagcccgtg gcccgcacca cgacgaagcc cgcgaagcag
      721 gcgccgatcc ccgcacaggc gcccaagccg caggccaagg aggagacgga gtccgctgag
      781 gaggacaagg tcacgccgct gcgcggcatg ccgaagaccc tcgccgcgaa catggaccag
      841 tctctgacgg tcccgaccgc gaccagcgtc cgcaccatcc cggccaagct gatgatcgac
      901 aaccgcatcg tcatcaacaa ccacatgtcc cgcacccgcg gcggcaaggt ctccttcacg
      961 cacctgatcg gctgggcgct gatcaagacg ctcaaggagt tccccagcca gaacgtgttc
     1021 tacgccgaga tcgacggcaa gccgtcggtg gtcgcgcccg cacacgtcaa cctcggcatc
     1081 gcggtggacg tccccaagcc cgacggcacg cgcgccctgc tggtccccag catcaagcgc
     1141 gccgacacca tgaccttcag cgagtacctg ctcgcctacg aggacctcat cgcgcgcgcc
     1201 cgcaacaaca agctgacggc ggcagacttc gccggcacca cggtctcgct cacgaacccc
     1261 ggcggcatcg gcaccgtgca ctccgtgccg cgtctgatga agggccaggg cgccatcatc
     1321 ggcgccggcg cgctgaacta cccggccgag ttccagggct ccagcgagaa ggtgctcacc
     1381 gagctgggca tcggcaaggt catcaccctc accagcacct acgaccaccg tgtcatccag
     1441 ggcgccggct cgggtgagtt cctgaagaag gtgcacgagc tgctgatcgg cgagcgcggc
     1501 ttctacgacg acatcttcgc cgcgctgcgc atcccctacc agccgatccg ctggaacgcg
     1561 gacatcgccg tcgacctcgc cgagcgcgtc gacaagcagg cccgggtgca ggagctgatc
     1621 aactcctatc gcgtgcgcgg gcacctgatg gccgacatcg acccgctcga gtacgtgcag
     1681 cgctcgcacc ccgacctgga gatcgagagc cacggcctga ccttctggga cctcgaccgc
     1741 gagttcgtca ccggaggctt cggcggcaag cgcgtcatga agctgcgcga gatcctcggt
     1801 gtgctccgcg actcgtactg ccggaccctc ggcatcgagt acatgcacat ccaggacccg
     1861 gagcagcggc gctggttcca ggagaaggtc gaggtcaagt acgtcaagcc cggccacgac
     1921 gagcagctgc gtgtgctcag caagctcaac gaggccgagg ccttcgagac cttcctgcag
     1981 acgaagttcg tcggccagaa gcgcttctcg ctcgagggcg gcgaatcgct gatcccgctg
     2041 ctcgacgaga tcctgcaggg cgccgcgaac gcaggcctgg acggagccgc catcggcatg
     2101 gcccaccgcg gccgcctgaa cgtgctcacc aacatcgcgg gcaagaccta cggtcaggtg
     2161 ttccgggagt tcgagggatc gctcaccccg ggcaaccagc gcggatccgg tgacgtgaag
     2221 taccacctcg gcaccgaggg caccttcgtc gccgaggacg gcgccgagct gcccgtgtac
     2281 ctcgccgcca acccctccca cctggagacg gtcgatggcg tcctggaggg catcgcccgc
     2341 gccaagcagg accgcaagcc gatcggcacc ttcacctggc tgccgatcct cgtgcacggc
     2401 gacgccgcgt tcgccggcca gggcgtcgtg gtcgagaccc tgcagatgtc gcagctgcgc
     2461 ggctaccgca ccggcggcac cgtgcacatc gtggtgaaca accaggtggg cttcacgacg
     2521 ctgccgaccg actcgcgcac ctcggtgtac gcgaccgacg tcgccaagac gatccaggcc
     2581 ccgatcttcc acgtgaacgg cgacgacccc gagtcggtca tccacgtcgc acagctggcg
     2641 ttcgagtacc gcgagcgctt ccaccgcgac gtcgtgatcg acctggtctg ctaccgccgc
     2701 cgcggtcaca acgagggcga cgacccgtcg atgacccagc cgctgatgac cgacctcatc
     2761 caggcgaagc gctcggtgcg caagctgtac accgaggccc tggtgggacg cggtgacatc
     2821 accgaggagg agtacgacaa ggccaaggcc gacttccaga accgtctgga gatcgcgttc
     2881 gccgagacgc acgccgccga gaccggcgcc accccgatcg cccccgagct gacccaggtc
     2941 gaccctcagg tcggcgagcc cgaggtcacc ggcgtgccgc tcgaggtcat ccatctcatc
     3001 ggcgacgcgc acgcgaacaa gcccgacggc ttcacggtgc atcccaagct gcagcagcag
     3061 ctcgacaagc gcgtcgagat gagccgcacg ggcggcatcg actggggctt cggcgagctg
     3121 ctcgcgttcg gctcgctgct ggtcgagggc acccccgtgc gcctggccgg ccaggactcc
     3181 cgccgtggca cgttcgtgca gcgccacgcc gtgctgcacg accgcgagaa cggccaggag
     3241 tggctgccac tgacgaacct gtcggcctct cagggacggt tcttcgtgta cgactcgctg
     3301 ctcagtgagt acgccgcgct gggcttcgag tacggctact cggtcgagaa caccgaggcc
     3361 ctggtgctgt gggaggcgca gttcggcgac ttcgtgaacg gcgcccagtc ggtcatcgac
     3421 gagtacatct cggctgccga gcagaagtgg ggccagcagt ccggcgtggt cctgctgctg
     3481 ccgcacggct acgagggcca gggaccggac cactcctccg ctcgcatgga gcgcttcctg
     3541 cagctgtgcg cccaggacaa catgaccgtg gcgcggccct ccacacccgc gtcgtacttc
     3601 cacctgctgc gtcgtcaggc gtacacgcgt ccacgcaggc cgctgatcgt cttcaccccg
     3661 aaggccatgc tgcgtctgcg cggtgccacc agccaggtcg aggacttcac gcagggacgc
     3721 ttcgagccgg tcatcgacga cgaccgcgct ctggaccgcg ccgcagtgaa gcgcgtcgtc
     3781 gtgcacgccg gcaagatcca ctgggacctc gccagcgagc tggagaagaa cccgaacccc
     3841 gagatcgccc tggtgcgcct cgagcagttc tacccgacgc cgatcgatgc gctgaaggcc
     3901 atcacggact cgtaccccaa cgccgagctg gtgtgggcgc aggacgagcc ggagaaccag
     3961 ggcgcctggc cgttcctggc gctggcgttc gccgaggtgc ccggcccgcg gcagttccgc
     4021 gtggtgtccc gtccggccgc ggcctcgccg tcgaccggat cgtcgaaggt tcacgcggcc
     4081 gagcaggccg cgctgctgaa gcaggcgctg acgctggact gagccgcgcc gcagtcgaca
     4141 ggatgccgcc cctcctcgcg aggggcggca tccttcgtct caacgggggg tcgtgagagc
     4201 tcagcgggtc gcgcgtcagc ctaggagcgc gaggtcctg
//
LOCUS       DATMSX010000410         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_44560,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000410 DATMSX010000000
VERSION     DATMSX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44560"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VN041_12895"
     CDS             complement(<1..916)
                     /locus_tag="VN041_12895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWU29971.1"
                     /translation="MVITERGLVRGEQRRERRRPAPRAVVLPAGIGVAVALVDYIGAG
                     IPSYWGDEAASVMSGQRSMPSLLQELSAVDAVHGLYYMLLHVWMWAFGTGEWATRALS
                     AIAIGALAAGVFTLGRMWFDRRTAIVAGVLVAVIPRASALAIETRGYAITAAAVVWLT
                     VLFSHLLRQGAPRRRWVLFGVLAGLCSWFFLYLLLVPLALSAVTFLAPRRGTVLRRGV
                     RGLRGDLTAAAAATLIAALPIITLAAFQRNQVSFLAHRGYLSPRGVLVTPWFTHGAVA
                     AVMWVLIAVGVAFALRRPVERASARLAVT"
     gene            1026..1370
                     /locus_tag="VN041_12900"
     CDS             1026..1370
                     /locus_tag="VN041_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWU29972.1"
                     /translation="MEMDAEEFERLVVDELDLLPDDMVDGLENVVFVVEDAAEDGEEL
                     FGLYEGFALTERGQYGMGELPDRIIVYREPHLAECDDEEQLRDEIHTTLVHEIAHFFG
                     IDDEQLHELGWA"
     gene            1367..1657
                     /gene="clpS"
                     /locus_tag="VN041_12905"
     CDS             1367..1657
                     /gene="clpS"
                     /locus_tag="VN041_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWU29973.1"
                     /translation="MSGPASVPVMDEQLTAAPLVPWELVVWDDPVNLMSYVARIFRTY
                     FGYPQEKATRLMLAVHNDGHAVVATGPRETMEVHAQAMHGFGLWATVREAGE"
     gene            1654..2124
                     /locus_tag="VN041_12910"
     CDS             1654..2124
                     /locus_tag="VN041_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HWU29974.1"
                     /translation="MTDAALSVPFASIEGRHLIALLDDFIALLDAPGDTADEALERLA
                     PDAYPDDPAAAEEYRRGTRDDLVDQRISDARTVRTALERFETDAAADIVDVPRAELDG
                     WLRTLSAMRLVLASRLGIDRDDGHDPDDPRYGTYDWLGYRLELLVQLADRLDEE"
     gene            complement(2132..2713)
                     /locus_tag="VN041_12915"
     CDS             complement(2132..2713)
                     /locus_tag="VN041_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29975.1"
                     /translation="MLSRSDDPLDSALTTATARIRDAIIAVQASNPVVVIDGRSGAGK
                     SSLAARVMRAWPLRTPAQLIALDSIYPGWSGLEAGAEIARESVLVPHGRGLLGTWHRW
                     DWEREEEAEAHAVDPALGLVLEGCGALTPASARLADVTVWVDGPHASRRTRALERDGD
                     GFRPFWDMWAAQEDAHMARHDPERLASISIRTP"
     gene            complement(2697..3014)
                     /locus_tag="VN041_12920"
     CDS             complement(2697..3014)
                     /locus_tag="VN041_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29976.1"
                     /translation="MPNIALKLGEQAASFGVKGAYGEALEIDGVSVTPVALTVTGFAG
                     GGVEQGEGGTGGGIAVPLGVYVRREEGLRFEPNIVSLLAVAVPFVWVLGRALSRVIRA
                     LKK"
     gene            complement(3053..>3589)
                     /locus_tag="VN041_12925"
     CDS             complement(3053..>3589)
                     /locus_tag="VN041_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWU29977.1"
                     /translation="MQQLARGEDARAVQTERVEKSVGHEETFENDIADADLLRSELLR
                     LADRVGARLRSSGWQAGAVAIKIRFADFSTLSRTVSLPEPTEVGQRIGETAVALFDQI
                     ARRDPVRLVGVRAEKLRETSGGALALWDDDEDQRRLEGTLDDARARFGVGMITRARHV
                     GRSGARPAGEQPRPFGRE"
BASE COUNT          579 a         1330 c         1215 g          465 t
ORIGIN      
        1 aggtcaccgc gagacgcgcg ctcgcacgct cgacgggccg gcgcagcgcg aacgcgacgc
       61 cgacggcgat caggacccac atgacggcag cgaccgcgcc gtgcgtgaac cagggggtga
      121 cgagcacgcc cctcggcgac agatagcccc ggtgcgccag gaagctcacc tggttgcgct
      181 ggaaggccgc cagagtgatg atcggcagcg ctgcgatcag cgtcgcggcg gccgccgccg
      241 tcagatcgcc ccggagtccg cgcacgccgc gtcgcagaac ggtgccgcga cgcggtgcga
      301 ggaacgtcac cgcagagagc gcgagcggca cgaggagcag gtagaggaag aaccacgagc
      361 acagcccggc gagcaccccg aacagcaccc agcgacgcct cggagcgccc tgccgaagca
      421 gatgcgagaa cagcacagtc agccagacga cggccgctgc ggtgatggca tagccgcgcg
      481 tctcgatcgc cagcgcgctg gcgcgcggaa tgaccgccac cagcacgccg gcgacgatcg
      541 cggtgcgccg gtcgaaccac atccggccga gcgtgaacac gcctgcggcc agtgcgccga
      601 tggcgatcgc gctgagcgcc ctggtcgccc actcccccgt gccgaacgcc cacatccaga
      661 cgtgcagcag catgtagtac aggccgtgca cggcatccac cgcgctcagc tcctgcagca
      721 ggctgggcat cgaccgctga cccgacatga cgctcgcggc ctcgtcgccc cagtaggacg
      781 ggatgcccgc gccgatgtag tcgacgagag cgacggcgac cccgatcccc gcgggcagca
      841 ccacagctcg cggcgccggc cggcgacgtt cgcgacgctg ctcgccccgg acgagccctc
      901 tctccgtgat gaccatgaga cgagccaatc gccggccgat ccgcgcatcc tatgaaatcg
      961 ggcctcccgg catgagagtt ctcacacgaa cgaccggaag ccctgtgcct ggatagcatc
     1021 gaggtatgga gatggatgcc gaggagttcg agcggctggt ggtcgacgaa ctcgatctgc
     1081 tccccgacga catggtcgac ggcctcgaga acgtggtgtt cgtggtcgag gacgccgcgg
     1141 aggacggcga ggagctgttc ggcctgtacg agggcttcgc actgaccgag cgcgggcagt
     1201 acggcatggg cgaactcccc gaccggatca tcgtgtaccg cgaaccccat ctggccgagt
     1261 gcgacgacga ggagcagctg cgcgacgaga tccacacgac cctcgtgcat gagatcgcgc
     1321 acttcttcgg catcgacgac gagcagctcc atgaactggg gtgggcatga gcgggccggc
     1381 atccgtcccg gtgatggacg agcagctgac agccgcaccg ctggtgccct gggagctggt
     1441 cgtgtgggac gacccggtga acctgatgag ctacgtcgcg cgcatcttcc gcacctactt
     1501 cggctacccg caggagaagg ccacccggct catgctggcg gtgcacaacg acggacacgc
     1561 ggtcgtcgcg accggtccgc gcgagaccat ggaggtgcac gcccaggcga tgcacggctt
     1621 cgggctgtgg gcgacggtgc gggaggccgg cgaatgaccg atgccgcgct gtccgtcccg
     1681 ttcgcgagca tcgagggtcg tcacctcatc gcgctcctgg acgacttcat cgcgctgctg
     1741 gatgcacccg gcgacacggc ggacgaggcg ctggagcgcc tcgcgcccga cgcgtacccc
     1801 gacgaccctg cggccgccga ggagtaccgg cgcggcacgc gcgacgacct cgtcgaccag
     1861 cggatctcgg acgcgcggac cgtccggacg gccttggagc ggttcgagac ggacgccgcc
     1921 gccgacatcg tcgacgtgcc ccgagcggaa ctggacggct ggctgcgcac gctctcggcg
     1981 atgcggctcg tcctcgcctc gcgcctgggc atcgatcgtg acgacggaca tgatcccgac
     2041 gacccccgct acggcacgta cgactggctg ggctatcggt tggagctgct cgtccagctc
     2101 gccgaccgtc tggacgagga gtgacccggc actacggcgt gcggatgctg atcgaggcga
     2161 gacgctcggg gtcgtgacgc gccatgtgcg cgtcctcctg cgccgcccac atgtcccaga
     2221 acggccggaa cccgtctccg tcccgctcca gcgccctggt gcgccgggac gcatggggac
     2281 cgtcgaccca caccgtcaca tccgccagac gcgccgacgc cggcgtcagc gcaccgcagc
     2341 cctccagcac caggcccagc gccgggtcga ccgcgtgcgc ctcggcctcc tcctcgcgct
     2401 cccagtccca gcggtgccag gtgcccagca gtccgcgtcc gtgcggcacc agcaccgact
     2461 cgcgggcgat ctccgcaccc gcctcgagcc cgctccagcc cggatagatc gagtccagcg
     2521 cgatcagctg cgccggcgtg cgcaaaggcc acgcccgcat cacccgcgcg gccaggctgc
     2581 tcttgcccgc accgctgcgt ccgtcgatca ccaccacggg gttggacgcc tgtaccgcga
     2641 tgatcgcgtc gcggatgcgc gcggtcgccg tggtcagcgc ggaatcgagc gggtcgtcac
     2701 ttcttgagag cacggatcac acggctgaga gcgcggccga gcacccacac gaagggcacg
     2761 gcgacggcca gcagtgagac gatgttgggc tcgaaccgca gcccctcctc gcggcggacg
     2821 tacacgccga gcggaacggc gatgccgcca ccggtgccgc cctcgccctg ctccacgcca
     2881 ccgccggcga agcccgtgac ggtcagtgcg acgggcgtga cgctcacgcc gtcgatctcg
     2941 agggcttcgc cataggcgcc cttcactccg aaggatgcgg cctgctcgcc cagcttcagt
     3001 gcaatgttcg gcatcccccc acgctagccg acgcacagga cccgcgcgac gctcactccc
     3061 ggccgaacgg cctcggctgc tcccccgcgg gccgagcgcc ggatcggccg acatgcctgg
     3121 cccgcgtgat catgccgacg ccgaaccgcg ccctggcgtc gtcgagcgtc ccctccagcc
     3181 ggcgctggtc ctcgtcgtcg tcccacagcg cgagcgcgcc gcccgacgtc tctcggagct
     3241 tctcggcgcg cacccccacc agacgcacgg gatcgcgcct cgcgatctgg tcgaacagtg
     3301 cgacagcggt ctccccgatc cgctggccca cctcggtggg ctcgggcaag gacaccgtgc
     3361 ggctgagggt ggagaagtcg gcgaagcgga tcttgatcgc cacggcgccc gcctgccagc
     3421 cgctcgagcg caggcgagcg ccgacccggt cggccaggcg cagcagctcg gagcgcagca
     3481 gatcggcatc cgcgatgtcg ttctcgaagg tctcctcgtg tccgacgctc ttctccacgc
     3541 gctcggtctg cacagcgcgg gcgtcctcgc cgcgggcgag ctgctgcat
//
LOCUS       DATMSX010000411         5846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_100905,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000411 DATMSX010000000
VERSION     DATMSX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100905"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VN041_12930"
     CDS             complement(<1..182)
                     /locus_tag="VN041_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29978.1"
                     /translation="MSTSEENGRTLTRKQLREIRLTGSTPVITEEEAASAAAQPPTTV
                     LPRAAQPIEIAPAPTA"
     gene            complement(179..781)
                     /gene="nadD"
                     /locus_tag="VN041_12935"
     CDS             complement(179..781)
                     /gene="nadD"
                     /locus_tag="VN041_12935"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWU29979.1"
                     /translation="MQPSTARAPRIGVMGGTFDPIHHGHLVAASEVAHSFDLDEVVFV
                     PTGEPWQKQNVSPSEHRYLMTVIATASNPRFTVSRVDIDRDGPTYTIDTLRDLKAERP
                     DAELFFITGADAVAQILSWRDHDELWDLAHFVAVSRPGHVLSTDGLPTDDVSQLEVPA
                     LTISSTACRERVNEGQPVWYLVPDGVVQYIAKHHLYRSKA"
     gene            complement(789..998)
                     /locus_tag="VN041_12940"
     CDS             complement(789..998)
                     /locus_tag="VN041_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29980.1"
                     /translation="MNLVAQIAMTAAASESHNVMGQTVIFGIIAAIIFAALALVTLSY
                     RNVANRHSAKADAYAEKHGEDGHGH"
     gene            complement(1048..2325)
                     /locus_tag="VN041_12945"
     CDS             complement(1048..2325)
                     /locus_tag="VN041_12945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWU29981.1"
                     /translation="MSTPAAGDLTADSAQQRLARAKEASRSIARLTSGEKGAALESIA
                     RAIESNVERIVAANGLDIEHGRENAIGDALIDRLRLDEKRVTALASAVRDVAALPDPI
                     GQVLGGHRMPNGIALEQVRVPFGVVGAIYEARPNVTVDIAALALRAGNAVVLRGGSAA
                     RMSNTVLVELMREALEAVGVDREAIQTVDDFGRDGAKALMHGRGFVDVLVPRGSASLI
                     ETVVTESTVPVIETGAGVVHIVLDESAPLDWARDIVVNAKVQRPSVCNAVETVLVLRQ
                     AAPLLVPVVASALQSEGVAVHGDDVVAGLVSGVIPATEEDWATEYLSLDISMKVVDTL
                     DDALAHIRKYSTGHTESIITTDSRNIERFLAEVDSAVVMANASTRFTDGGEFGFGAEV
                     GISTQKLHTRGPMGVSELTSTKWLARGTGQTRG"
     gene            complement(2381..3169)
                     /gene="proB"
                     /locus_tag="VN041_12950"
     CDS             complement(2381..3169)
                     /gene="proB"
                     /locus_tag="VN041_12950"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWU29982.1"
                     /translation="MSAQTRADLATAGRIVVKVGSSSISGEASWRIPMIVQALASAHR
                     RGVEVILVSSGAIATGIPVLDLDSRPNDLATQQAAAAVGQNLLVFRYQEALRPFRIVA
                     GQVLLTTGDLENATSRSNARRAMDRMLALGVLPIVNENDTVATQEIRFGDNDRLAALV
                     AQLLHADALVLLSDVDSLYTKPPTDPDARPIDTVAPDADLAGLEFGASVVNSVGTGGA
                     ATKVSAARMAAASGIGVMVTSADLIGKALEGAAIGTWFEPAVTA"
     gene            complement(3166..4665)
                     /gene="obgE"
                     /locus_tag="VN041_12955"
     CDS             complement(3166..4665)
                     /gene="obgE"
                     /locus_tag="VN041_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWU29983.1"
                     /translation="MVTFVDTVTLHLRAGKGGNGCVSVKREKFKPLGGPDGGNGGDGG
                     DIVLVADPQVTTLLSYHHSPHRSSGNGGFGMGDHRSGYAGETLELPVPLGTVVKSVDG
                     EVLHDMVTPGERYVVAEGGRGGLGNASLASPKRKAPGFALLGTPGYEGAIQLELKTVA
                     DVALVGYPSAGKSSLIAAMSAARPKIADYPFTTLHPNLGVVQAGDSRYTVADVPGLIE
                     GASEGRGLGLEFLRHVERCSALLHVLDCATLEPGRDPLSDLDVILAELAAYEVPEGQI
                     PLLERPQLVALNKVDVPEARELADMVRADIEARGFRVFEISTVSREGLRPLSFALAEL
                     VDEHRAATAVETPPERVVIRPKGAKEEFTIRVEGGTYGNVYRVIGEKPVRWVQQTDFQ
                     NEEAVGYLGDRLERLGVEDALVKAGAEPGSTVMIGTGDNSVVFDWEPSIASTAELMSS
                     PRGTDIRIGGSSRRTTNERREKYYERMDAKAAARAELEEQRLASREGEE"
     gene            complement(4766..5023)
                     /gene="rpmA"
                     /locus_tag="VN041_12960"
     CDS             complement(4766..5023)
                     /gene="rpmA"
                     /locus_tag="VN041_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWU29984.1"
                     /translation="MAHKKGASSTRNGRDSNAQRLGVKRFGGQAVNAGEILVRQRGTH
                     FHPGVNVGRGGDDTLFALESGAVQFGTKGGRKVVNIVAAAQ"
     gene            complement(5044..5352)
                     /gene="rplU"
                     /locus_tag="VN041_12965"
     CDS             complement(5044..5352)
                     /gene="rplU"
                     /locus_tag="VN041_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWU29985.1"
                     /translation="MVYAVVRAGGRQEKVEVGTIVQLDRVQAAQGEKIELPAVLLVDG
                     ATVTTDADKLAKVKVTAEVLGDLRGPKIIIQKYKNKTGYKKRQGHRQDLTRVKITGIK
                     "
     gene            5567..5833
                     /locus_tag="VN041_12970"
     CDS             5567..5833
                     /locus_tag="VN041_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HWU29986.1"
                     /translation="MTVLVDDPIWPAHGRLWAHLVSDADLGELHAFAAAQGLPRRAFD
                     LDHYDVPEDALPRLLAAGAVHVGGKELTRRLIASGLRVRGRDRR"
BASE COUNT          839 a         1982 c         2033 g          992 t
ORIGIN      
        1 tccgcggtcg gggccggcgc gatctcgatc ggctgcgcgg cacgcggcag caccgtggtg
       61 ggcggctgcg cggcagcgga cgccgcttcc tcctcggtga tgaccggcgt cgagccggtc
      121 agacggatct cacgcagctg cttgcgcgtg agggtccttc cgttctcttc cgatgtgctc
      181 atgccttgct ccgatacaga tgatgctttg cgatgtactg gacgacccca tccggcacca
      241 gataccagac gggctgcccc tcattgaccc gctcacgaca cgccgtcgac gaaatggtca
      301 gtgccggcac ctccagctga ctcacgtcat ccgtgggcag tccgtcggtg ctgagcacgt
      361 gtccgggtcg ggagaccgcg acgaagtggg cgagatccca cagttcatca tggtccctcc
      421 aactgagaat ctgcgctacg gcatccgctc ccgtgatgaa gaacagctcc gcgtcgggcc
      481 gttcggcctt cagatcgcgg agcgtgtcga tcgtgtaggt gggcccgtcg cggtcgatgt
      541 cgacgcggct gacggtgaat cggggattgg atgcggtggc gatgaccgtc atcagatagc
      601 ggtgctcact gggtgagacg ttctgcttct gccacggctc accggtgggg acgaacacga
      661 cctcatcgag gtcgaacgag tgcgccacct cgctcgccgc gaccaggtgc ccatggtgga
      721 tggggtcgaa agtaccgccc atcacgccga tgcgcggtgc tcgagcagtg gatggctgca
      781 tgtgcgcctt agtgaccgtg gccgtcctcg ccgtgcttct cggcgtatgc gtcggccttg
      841 gccgagtgac ggttggcgac gttgcggtac gacagcgtga ccagggccag cgccgcgaag
      901 atgatcgccg cgatgatgcc gaagatcacg gtctgtccca tgacgttgtg ggactcggac
      961 gcggcggcgg tcatcgcgat ctgggcgacg aggttcattc tggctccgtt tctgggcggt
     1021 cgggcgagcg cctgacaagt ctagcgctca gccccgggtc tggcccgtcc cccgcgccag
     1081 ccatttggtg ctggtcagct cggagactcc catcggcccg cgggtgtgca gcttctgtgt
     1141 ggagatgccc acctcggcgc cgaatccgaa ctccccgcca tccgtgaacc tggtcgacgc
     1201 gttcgccatc acgacagcgg agtcgacttc ggccaggaag cgctcgatgt tgcgggagtc
     1261 ggtggtgatg atcgactccg tgtgccccgt gctgtacttc cggatgtgcg cgagcgcatc
     1321 gtcgagcgtg tccacgacct tcatggagat gtccaggctc aggtactcgg tggcccagtc
     1381 ctcctcggtc gccgggatga caccggacac caggccggcg acgacgtcat cgccgtgcac
     1441 cgccacaccc tcgctctgca gcgcgctggc gacgaccggc accagcagcg gagccgcctg
     1501 gcgcaggacg aggaccgtct cgacggcgtt gcagacgctc gggcgctgca ccttcgcgtt
     1561 cacgacgatg tcgcgcgccc agtccagcgg ggcggattcg tccagcacga tgtgcaccac
     1621 gccggcgccg gtctcgatca cgggcacggt cgactcggtg accaccgtct cgatgaggga
     1681 cgcactcccc cgcggcacca gcacgtcgac gaacccgcgg ccgtgcatga gcgccttcgc
     1741 accgtcccgg ccgaagtcgt ccacggtctg gatcgcttcg cgatccacgc ccacggcctc
     1801 cagagcctcg cgcatcagct ccaccaagac ggtgttggac atccgcgcgg cactgcctcc
     1861 tcgcagcacc acggcgttgc ccgcccgcag cgcgagcgcc gcgatgtcga cggtcacgtt
     1921 ggggcgggcc tcgtagatcg cgcccaccac accgaacggc acgcgcacct gctcgagggc
     1981 gatgccgttc ggcatccggt ggccgcccag cacctgtccg atcggatcgg ggagcgccgc
     2041 cacgtcgcgc acagcggatg ccagtgccgt cacccgcttc tcgtccagac gcagccggtc
     2101 gatcagggca tctccgatgg cgttctcccg gccgtgctcg atgtccagcc cgttcgcggc
     2161 gacgatgcgc tcgacgttcg actcgatcgc gcgcgcgatg ctctccagcg ccgcgccctt
     2221 ctcgccgctg gtgaggcggg ctatcgatcg cgacgcctcc ttggcgcgcg cgaggcgctg
     2281 ctgtgcggaa tcggcggtga ggtcgcctgc ggcgggagtg ctcatccggt cagtgtatcg
     2341 acggcggaac ggatgccggc gccgtgtgac ccacggggga tcaggccgtg acggcgggct
     2401 cgaaccaggt gccgatagcg gcgccctcga gcgccttgcc gatcagatcc gcgctggtca
     2461 ccatcactcc gatgccggat gcggcggcca tccgcgcggc ggagaccttg gttgccgctc
     2521 ccccggttcc gacgctgttc acgacggatg ccccgaattc gagtccggcc aggtcggcgt
     2581 cgggcgcgac ggtgtcgatc ggccgcgcgt ccgggtcggt cggcggcttg gtgtacagcg
     2641 agtccacgtc gctgagcagc accagggcgt cggcgtggag cagctgggcg acgagggcgg
     2701 cgagccggtc gttgtcgccg aagcggatct cctgtgtggc gacggtgtcg ttctcgttga
     2761 cgatcggcag cacgccgagc gccagcatcc ggtccatcgc gcgccgggcg ttgctgcgcg
     2821 aggtcgcgtt ctccaggtcg ccggtggtca gcagcacctg tcccgcgacg atccggaagg
     2881 gacgcagcgc ctcctggtac cggaacacga gcaggttctg cccgaccgct gcagcggcct
     2941 gctgggtcgc caggtcgttg gggcgcgaat cgaggtcgag caccgggatg cccgtggcga
     3001 tggcacccga ggaaaccagg atcacctcga cgccgcggcg gtgcgcgctc gcgagggcct
     3061 ggacgatcat cgggattcgc caggatgcct cgccgctgat ggacgaggaa ccgaccttga
     3121 cgacgatgcg cccggcggtg gcgaggtctg cgcgggtctg cgcgctcatt cctcgccctc
     3181 gcgggatgcg agtcgctgct cctcgagctc cgcgcgggcg gcagccttgg catccatccg
     3241 ctcgtagtac ttctctcggc gctcgttggt ggtgcggcgg ctgctgccgc cgatgcggat
     3301 gtcggtgccg cgcggcgagc tcatcagctc ggccgtggag gcgatggacg gctcccagtc
     3361 gaacaccacg ctgttgtcgc cggtgccgat catgaccgtg gagccgggct ctgcaccggc
     3421 cttgaccagc gcatcctcca cgccgaggcg ctcgagacgg tcgccgaggt agcccacggc
     3481 ctcctcgttc tggaagtcgg tctgctgcac ccagcgcacg ggcttctcgc cgatcacacg
     3541 gtagacgttg ccgtacgtgc cgccctcgac acggatcgtg aactcctcct tcgcgccctt
     3601 cgggcggatc acgacgcgct cgggcggggt ctcgacggcc gtggccgcac ggtgctcgtc
     3661 gacgagctca gccagggcga agctgagcgg acgcagtccc tcgcgcgaga cggtcgagat
     3721 ctcgaacacg cggaagccgc gcgcctcgat gtcggcgcgg accatgtcgg ccagttcgcg
     3781 cgcctcgggc acgtcgacct tgttcagggc gaccagctga ggacgctcca ggagcgggat
     3841 ctgtccctcg gggacctcat aggcggcgag ctcggcgagg atcacgtcca gatcggagag
     3901 cgggtcgcgc ccgggctcca gagtggcgca gtccagcacg tgcagcaggg cgctgcagcg
     3961 ctcgacgtgg cggaggaact ccagtccgag tccgcgtccc tcgctggcgc cctcgatgag
     4021 gccgggcacg tcggcgacgg tgtatcggct gtcgccggcc tgcacgactc cgaggttggg
     4081 gtgcagcgtg gtgaacggat agtcggcgat cttgggccgc gcggccgaca tcgcggcgat
     4141 caggctggac ttgcccgccg agggatagcc caccagcgcg acgtcggcga cggtcttcag
     4201 ctcgagctgg atggcgccct cgtagccggg ggtgccgagc agcgcgaagc cgggcgcctt
     4261 gcgcttggga gaggccagcg acgcgttgcc gaggccgccg cgtccgccct cggcgacgac
     4321 gtagcgctcg ccgggcgtga ccatgtcgtg caggacctcg ccgtcgaccg acttcaccac
     4381 ggtgcccagc ggcacgggca gttcgagggt ctcgccggcg tagccggagc gatggtcgcc
     4441 catgccgaag ccaccgttac cgctgctgcg gtgcggcgag tggtggtacg acaggagcgt
     4501 ggtcacctgg gggtcggcga ccagcacgat gtcgccgccg tcgccgccgt tgccgccgtc
     4561 cggtccgccg agaggcttga acttctcgcg cttcaccgag acgcagccat tgccgccctt
     4621 gcccgcacgc aggtgcaggg tgacggtgtc cacgaacgtg accatgggtg ctccttgtga
     4681 agatggtccc tgagcctggc agaggggcct atacgcagga aggggcgagc cgaagcccgc
     4741 cccgttccga gagtgtgtgc tgcgcttact gagcggcagc gacgatgttg acgaccttgc
     4801 ggccgccctt cgtgccgaac tgcacagcgc ccgactcgag ggcgaacagc gtgtcgtcgc
     4861 caccacggcc gacgttcacg ccggggtgga agtgcgttcc gcgctgacgg acgaggatct
     4921 cgccggcgtt gacggcctgg ccgccgaagc gcttcacgcc gagtcgctgt gcgttggagt
     4981 cacgaccgtt gcgggtcgag ctcgcaccct ttttgtgtgc catttctcag ctcctcttcg
     5041 tgcttacttg atgccggtga tcttgacgcg ggtcaggtcc tggcggtggc cctggcgctt
     5101 cttgtagccg gtcttgttct tgtacttctg gatgatgatc ttcgggccac ggaggtcgcc
     5161 gaggacctca gccgtgacct tgaccttcgc cagcttgtcc gcgtcggtgg tcaccgtggc
     5221 accgtcgacg agaagcacgg cgggaagctc gatcttctcg ccctgggcag cctgaacacg
     5281 gtcgagctga acgatcgtac cgacctcgac cttctcctgc cggccaccgg cgcgcactac
     5341 tgcgtaaacc acttcatacc tgtttcgttg gggagcggac tgctccggga atctcacggg
     5401 aagacttctg cttgcgtacg acgccttcgc gaagggtgcg gacgcaagac ttctcctgcc
     5461 gcccggggag cagacagggg tctcgcacca aaggactact ttacctgaag cgaggccatg
     5521 ccgcaaagtg agcggcgggg tgtgtcacgg gccctaggat cgcgacatga ccgttctcgt
     5581 cgacgacccg atctggccgg cgcacggacg gctctgggcg cacctggtca gcgacgcgga
     5641 cctcggcgag ctgcatgcgt tcgcggcggc gcagggactc ccccggcgcg ccttcgacct
     5701 ggaccactac gacgtccccg aggacgcgct gccgcggctc ctcgccgccg gcgctgtgca
     5761 cgtcggcggc aaggagctca caaggcggct gatcgcctca ggactgcgcg tccggggcag
     5821 ggatcgacgc tgagtcggat gccgtc
//
LOCUS       DATMSX010000412        57022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176696,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000412 DATMSX010000000
VERSION     DATMSX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 57022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57022
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176696"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1060)
                     /locus_tag="VN041_12975"
     CDS             complement(170..1060)
                     /locus_tag="VN041_12975"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWU29987.1"
                     /translation="MTISGANCMIIVAAVFWLVSLAGGGRTLRVVLALLALGAFVVLV
                     TPEPSVVRAAVMAALAMLSILLGRPSAGLAMLSLAVCGLLIADPWFAGSPGFALSAAA
                     TGALLVLFAPLLAALGGKPGFRIDDAARFRMMQSFDAQSPNAPGLADIIDAAYLPVDT
                     PVTGYNARMAQLTHLFADPSRDQLLNTLRTRQAEVWSYRFDWARQPAPWNTVYGAAHA
                     FDLPFLFGNFGPSVFSRVTNSTANAPGRLALSNAMMASLKAFMQTGDPQNAALGQTWK
                     PWPSSLVFDADLGATRIRLQ"
     gene            1075..2607
                     /locus_tag="VN041_12980"
     CDS             1075..2607
                     /locus_tag="VN041_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWU29988.1"
                     /translation="MAQLIVREYLRVSKDPRGTGKSPDQQHVENVRSFEQQGWELHPS
                     APYRDTDRSASRYAKRNREDFRRLIADLEDDTFGADVLAIWESSRGSRRVGEWVDLID
                     LCKERQARIWVTTHGRLYDPANARDRRTLLEDAVDAEYESDKTSERIRRNVRAAAEAG
                     RPHGKNVFGYQRMYDAKTRELLRVEEHPEQAAIVREAARRVLAGETFYAIAKDFNVRG
                     IPPRRPTRVEHRKNHGWTPPAIKQMLTMPAYAGKRQHQGMIVSVALWPALIDYATWEK
                     LQAVMSPQQRRRTNDWPAKHLLSGIALCGVCGTPMRVGKQNAGSKKFDEDGNPLPREV
                     YGAYVCSGIPGRPGPDGKRGFHVAMRQEHLEEVVIELLFARLERPDFLTMIGDRGDAA
                     GAERDALLAEIAGYHSYLDRLREEATALLRFDILLDQEARIEPKIKAAQTKLEKLSEM
                     DPFVLTLLSTGAIRMQWDSLKLAEQRRVIRAVMAPRVNRIGKGWRGRKGLNYERVQPG
                     WR"
     gene            3343..3642
                     /locus_tag="VN041_12985"
     CDS             3343..3642
                     /locus_tag="VN041_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU29989.1"
                     /translation="MPLASTTLIDLPTAATRSDYGLSTLRRHIRAGSIPAVKVKGRIY
                     VRPEDLDAFAAPEPVHVPDVTLQEWAGRMAAKAPAFRPEQRNVIMSAFASALGGE"
     gene            3635..5446
                     /locus_tag="VN041_12990"
     CDS             3635..5446
                     /locus_tag="VN041_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HWU29990.1"
                     /translation="MSDSVTARESRPAANGAALEEFHGSTEELNTATQQTTQQTVRTT
                     PASFVPTTQESLVERTVTRYLMEIVDDPNISLRTVRDTLIRRINAEFDLENRGRKDSN
                     SYLPAIKKTPVLDEHTVTSVLLARHRVVAIDLAEGEAEDLTELAVYVDFGEAAGTYST
                     SKTRIRSLLSDLRPSMSANAIESAYARLRIHAPVVTRTIEPHLIPVANGVFDHSRQVL
                     REFSPDWVFLSKLPVDYDPGATNEHITMPDGDDWDVESWLQSLSDDEGVPELLWEIIS
                     AAVRSHAPWNRAIWFVSERGNNGKGTLIQLLRNLIGTKACSSIAFADFGREFLLEPLL
                     YSRVNLVDENDVGKLAESIAAWKAFVTGDPITLNRKHRTPVKIRWRGLDIQCHNSTIQ
                     RTKDRSGSFYRRLLIVPFNKWFGDSERAYIKTDYLTRAGVLRYVLKRALEMPHSSLST
                     PEACRRALDQYQGDNNYLLAFWLEFKDQFVWDLIPYRFLHALYVEWLRRVNPSGHPES
                     QAALTALLSAHLAGSDEWDAKRSDTRPGTAMALAEPLIDQYGLTDWMNHDYTGTDRSK
                     RCIVSPLRQNYKGLIRRTKSNHSAAGAASPVTSTDDD"
     gene            5887..7284
                     /locus_tag="VN041_12995"
     CDS             5887..7284
                     /locus_tag="VN041_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HWU29991.1"
                     /translation="MPVVVASSTRSADALISYALEDKPDQRGERYVMASGVGGMLVSV
                     AKHQMRDVRRKWAKDKPGAFVQAYHVIQSFARDELDPEQPDSWMTAQKLGRALAEERF
                     PGRQVLVVTQRDGRTGCVHNHLVANSIETKSGRSLNSAVVTHARLVEAHERVLDKQGF
                     AQRADLKQAFSDAKERQERGEPSSLRRAGSNANSELREFQQHILWETDCDLADEFGGP
                     RKKEPFSLTVLRHSIEMTLEDPAVVDWDSFVEVGRSRGVQIEQRGKKGRGISYGMLRE
                     EPDGTRAEPAASDRRRCTTLGTAYEMGAVEAAFVRNGIASIATARAGQTKNAQTAKQR
                     MLMALAEASAEGDDLAQRMIAEHRAQRPTVIDEQPESMAALETDPQDAISPSCTAVTV
                     LDESRQASRNEDEVNSLTDGLTAAAESQPSPIKTVREEAGGAARKVRSLHTRFPELFD
                     AERPAGQDTHHYGLE"
     gene            8031..10262
                     /locus_tag="VN041_13000"
     CDS             8031..10262
                     /locus_tag="VN041_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29992.1"
                     /translation="MAALFGAAVAVGATLLGLYYATVGVIASTIYKSVPGDVRDLFLQ
                     ERNNETYLRVVILTIASGIAALILSVFGRPVSGLTLLAVAGLTALTCIGHVVVTRRLL
                     EYFDPSKLALPLMRQLATAIQTATAEKSRDISFRQTEAHASALRAATSYRHLAQLLEE
                     SELRNATAPVVLSRQLIYILKLYSSRKHAIPTSSNWWSRVPRHQNWLTPSQNSLDLAL
                     NTSVGLPPELQPDYLWFENAIGRVLGRSLKVAFQAQGGADALAVSEEVADLVGHLTAR
                     LQIDEAVTIATMWERVVMAVVATPQVAAADADDNEVRLNQMAAAESVVRPVTVMLLGL
                     SHAASSLIQRDLPTEFEAALSKNDVLSVEALPTETRQMLESFAAAIRREVASEGRRVT
                     PAWWVNHLAARSLADMLLQTESGIIALIKDRALEHVSEFDEAGRPDLAVVAGMAALEL
                     LNKLDHHEQIVRRAEEKLGSFRNSNIGIERWPARTESLDAGSEHRALLGRLASLLPKL
                     RKAEFDPREPDLYGQLYQFVVDGAFHAILDGDADRGLSMYTAALAEMESARARIMSDL
                     KQQDAQIRLVYAMEPLITAMDLAGYALLMHELDGGDIWPEVKGKWDQLLELSPQLPSF
                     LLMAAKFVEDIYGLTVGGVERSRRSIALGQIFKERNLSHEWETLLEEANEPLHPSPIV
                     SAIAPRGLGIQDDLYALFIAEYLATFLENGEDLGNKARLIAEEIERYRAEQPDDDTED
                     GQG"
     gene            10259..11254
                     /locus_tag="VN041_13005"
     CDS             10259..11254
                     /locus_tag="VN041_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29993.1"
                     /translation="MMHDDAFAGLVADWSEQIPYEARPETPAPRRREIPDPAGKLHMF
                     LQDLRTRALPYGFGANDGFGLRLAPEDADVARLVTNALPGRAYPHSQLERAFRDYVET
                     ALWYLTFGEVFLKLVYYRPADRPDSRPVGFRIEFLDPERVQRRFGRYHYWTLKLHPGH
                     DVAEWKRTPLPTEGLVVVSLPRRLRRMMDRAVKIVDATNQDLKVMSEFTMGRFAGEPA
                     FDIGRYNRQIADVVLRETRAVGWGGRGLLTEGMLDPFKAWRNVQFARFDAKLRDVAVD
                     GLQDAINRAGTLMGFDATLQLSGVLGEADLDQLEHDLSAGVRPMSELFAPQPSGG"
     gene            complement(11518..12459)
                     /locus_tag="VN041_13010"
     CDS             complement(11518..12459)
                     /locus_tag="VN041_13010"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HWU29994.1"
                     /translation="MRDRSDAESPGPTVIALDVDGVLNSFGRKADPYFMTVHVGRWPI
                     RWRPAVMDRLRLLLLRPDVEGAWLTTWLEEPWLLDELEHVLGLDGIVLHRAGYPAIQT
                     TDGRSHIDPRFDAWGAFVPESPHWWKLRAAELLIEQLRPARFAWADDELDVSTRTPGS
                     RWRLGRTPAQFLVRPDPMVGLTEADLRRLEGWASEAHAGGEAAAIGVLLRDLIDGLGP
                     TLVAAFVGEKDCRVVQRWADGATPFPAAGLRERLVCAHRVWQRVAAAEGEDVARSWFV
                     GSTPWLHDDTVVAAIAEGRLTAVSIAAQALVDDSWSG"
     gene            12630..13265
                     /locus_tag="VN041_13015"
     CDS             12630..13265
                     /locus_tag="VN041_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29995.1"
                     /translation="MDGQDFDRTVNAQASATSVRALVDVISRLTDDPEWLIAALTEIM
                     RAMRAPVPPTEAEVEFLLSSAAFTPTQLSRIREEVARGSLTLAGAESFLAALHATWSL
                     EQVARYVDKTTDDVLTAVKVGKLYAVAIAERLRFPVFQFNIGRPEPLIPHLPELIEAA
                     GTQWSWISVVAFMETRQASLVAVAAQTPRAWLLDGGSFDEIKQIIDSAHWR"
     gene            13295..13594
                     /locus_tag="VN041_13020"
     CDS             13295..13594
                     /locus_tag="VN041_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29996.1"
                     /translation="MSTNTWRQEWPAHRVRLIDITARHFVDGQARASRGELPAWTELT
                     PEAQSNFCDNVAAVLAAQALAFAEIEAELSAKQARVSMNEATTRGADAAASETES"
     gene            13696..13956
                     /locus_tag="VN041_13025"
     CDS             13696..13956
                     /locus_tag="VN041_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29997.1"
                     /translation="MTAGEHVVALLAQHFEMPIFIPEPSLRNPDRYATSPHVTRGRDS
                     ISVRPPVEFGRLIKERARESGLYYGTYVVAALEEILHRQDKS"
     gene            13992..14804
                     /locus_tag="VN041_13030"
     CDS             13992..14804
                     /locus_tag="VN041_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWU29998.1"
                     /translation="METLESAGTLDLPDKRVWVIGDLHGNTGWIQTLFPAMRRFDPTM
                     RTVLQLGDYGFDHGGRGADAVDFWAKNAGIERVLVTLGNHEAWDKIAPAQDEAPGQAI
                     RVSEVVWFLPRPFRFTVAGREVLSLGGASSVDKAFRTVGKDWFEDELITDAMEHAAIS
                     TGRGDVLLTHESPIRSVPEVQSILTANPFEYSLEALHISASQRARVQRVSDATQPELH
                     LHGHMHVYGDVDLADGRRIVSLDRDTFARNAGTLDLSTLEFTPLPHAFIKQR"
     gene            complement(14995..15252)
                     /locus_tag="VN041_13035"
     CDS             complement(14995..15252)
                     /locus_tag="VN041_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU29999.1"
                     /translation="MDAQVNIPRATCASAMVVITVALEPTGQIRPVETRALDVDGTHD
                     EIVAALRAPTVNGWTINSITSRPVVLDGHKPTVVAADIGAM"
     gene            complement(15366..16061)
                     /locus_tag="VN041_13040"
     CDS             complement(15366..16061)
                     /locus_tag="VN041_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWU30000.1"
                     /translation="MCGRFAMDAKTDDLIHQFVADGGKPEDWWKDFHGSYSIAPTDDA
                     VIVRDRGEGRTLELVRWDWQKPANRPKGAPIINARIEKLDTGFWLPAFTAARCVVPMR
                     GYFEWTGEKGDKTPHFLHGDGLLAAAGLTWSMELPDGERSRCMVVITREARDAGGEVH
                     DRMPAFLTEDAMTAWLEPVKLDRGDRDGMLSLLDAASADVAGTITQYVIDRRVNNSRT
                     VDASDPSVIAPAA"
     gene            16708..16971
                     /locus_tag="VN041_13045"
     CDS             16708..16971
                     /locus_tag="VN041_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30001.1"
                     /translation="MTATNIEHTVTIWMVNEIPARMVYAGRRWRVTDTPTRLRHSIWT
                     VPLDEPHHGLYGWRFQGTDDDGLSLVFDVYRCEDGWHVHRAYD"
     gene            17014..17229
                     /locus_tag="VN041_13050"
     CDS             17014..17229
                     /locus_tag="VN041_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30002.1"
                     /translation="MANSEDVWWRLPDGVPIILTSVHRGRAYWEAFDGTTGSVTEPEF
                     ISTHRPIAVVYPPKIEGPSNSDGGSTS"
     gene            complement(17781..18152)
                     /locus_tag="VN041_13055"
     CDS             complement(17781..18152)
                     /locus_tag="VN041_13055"
                     /inference="COORDINATES: protein motif:HMM:NF017793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HWU30003.1"
                     /translation="MRIAVPHDKVAGLVAEMTIASKNIALVLDELDRESKLLQSQWTG
                     EAREAYRAAHAQWSAAHRGMNEVRRELARRLANTNQHSIDAHTLATGIWDLSPCHLAF
                     TAAEVRVRALAWEPMDISMGS"
     gene            complement(18156..18635)
                     /locus_tag="VN041_13060"
     CDS             complement(18156..18635)
                     /locus_tag="VN041_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30004.1"
                     /translation="MNAKKTHAQDSNGTFIRYFQPPMSTTTADYLRYVLGLVDGEKRT
                     TFSIWRLPEPGGDPMKRGETFIQAAGGSDKMAIEARVMTSDGTAHLYAVGRQEPHDDE
                     TTTVPISDKHVVRVFTNEIFTADEAAVIFMTFYLTDAVGRPYQLREIDLSVPQSEER"
     gene            19208..20299
                     /locus_tag="VN041_13065"
     CDS             19208..20299
                     /locus_tag="VN041_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30005.1"
                     /translation="MDMQLPLRAHGPSLTSDQARMLDDEFFHSNPLGHFSSKIANLLE
                     AERTSSHPTAESYSEFFDALGLTESEFLIHFDEQERRVQTAVEALALRHQAAEALTRF
                     TYAVAASTPNGSDARCVWLAIADSPTKMIDVLNANVDALNTEGRFLELFFPPGAVIDH
                     TAIRAADTAIAWLNHAAFLLSDNELSVNAANNKVKHGLAVSARGDVRIEFVTTPPDAD
                     GNIPASAFGEGKSVPLFDRAMLTYLCRPHSTPRQGVEAVSLRVDLPIVLAEAWMMANV
                     YAALFVSAARRHFGNDLPDSIAEYPRLVVDRLPEHVIGGRVLGFRSPITTPPDGATPH
                     RPIGLFFHRRFIPINVDFEHRFTGRVIKG"
     gene            20628..21581
                     /locus_tag="VN041_13070"
     CDS             20628..21581
                     /locus_tag="VN041_13070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWU30006.1"
                     /translation="MLRHTGPARHTQSWKSRRDPCDSSRHLQCCGFRHTTNRFLLATA
                     HEAQKGTPVIDSSPAALRVRPRVWIGLSIWIGYTAVVFLVQMIAGVPYTELGRDGATL
                     FFGAGLSLIVGAMLLALTTSLLGWWRPALFERKPSARWPIFVPAIMAVALVVNLASTD
                     WGSYDGAFFAASLVLLLVGFTEEMTARGLLLVSLRSRLGEGWVWFLTSALFGLMHLVN
                     AASGQDLLLTLQQVGMAFLGGTVLYILRRTTGTLIWAMVLLGLWDFSTFAVGHGTPSA
                     FAALAGTINLIAGVLGLIVVAFVIRGAGEKISTTKMAAVAA"
     gene            complement(21799..22572)
                     /locus_tag="VN041_13075"
     CDS             complement(21799..22572)
                     /locus_tag="VN041_13075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30007.1"
                     /translation="MTARLTDKIIIVTGAASGIGRGTALRLLEEGAHVAALDLREEDL
                     AALVEIAPEDTRSRLFVHRVDIANEESVRTAVADVVTHFGRLDAIVNAAGILYGDHTH
                     EMTLDRWNRLLQVNLTGTFLMVRESLPHLLATTSGVIINFASTSAFFAHPYMAAYAAT
                     KGAIASFTHSVALEYARHGLRAVNIVPGGITSGITNTTPQNLPADTDWTLLEHLGAPL
                     NDGRMGRPEDVAGVIAMLISEDGAFITGTEIRIDGGAHT"
     gene            complement(22584..25106)
                     /locus_tag="VN041_13080"
     CDS             complement(22584..25106)
                     /locus_tag="VN041_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWU30008.1"
                     /translation="MSRSPLNANWTYRPKTSIFADAHGQSSPGVPVHLPHDAIISLER
                     HADAPSGAAGAFFPGGAFEYRTTLDAPLDWQDKHVALEFEGVYRDAMIYVNGAFVAQR
                     PNGYAAFVVCLDGYLEYGAENTIRVDARTHADSRWYTGAGLYREVHLWVSERVHIPEQ
                     GGVQITTPDVDNERAVVSVTTRVTNSGTLTRTATVTTEILDPHGRVVGVERSPVTTLP
                     QTEGTVRQRIYVPTPERWSVDNPALYTARTTLDGISDVADTRHTRFGIRTLQLDPTHG
                     LRINGETVKLRGACIHHDNGILGAATYADAEERRVLLLKAAGFNAIRNSHNPMSVAML
                     DACDRHGMLVMDETFDMWAESKSPFDYSLAFPEWWERDVESMVATDFNHPSVIIYSIG
                     NEIPETGNAIASQWGRKLAEKVRELDSTRYVTNGINGFVSALSEISDMIEAATENASS
                     SSETEESEADGGVNALMGSTEDFMDWVATAPLVTAATAESFAILDIVGLNYGDARYEM
                     EREHPNRIVVGTETFPSRIDRNWKLVTEHPHVIGDFTWSGWDYLGEVGVGRVKYEGEP
                     DAFDGEFPWLTSWSADLDITGRRRPMSYYRETVFGLRSEPYIAVRRPSNHGRAFTLGQ
                     WSWTDAISSWSWNIDDGFPMTLEVYSDADEIRLELNDRVVAAERVGARRAFLADFEIP
                     FEAGTLTAIALRDGEEVGRSVLRSAAGDALLDVRLDKTTTELRDGALFFAEIELRDPE
                     GNLVNDADRPVTVTVNGAGSLAAFGSGRPRTTERFDENTHGTYDGRVLAVIRPDRAGA
                     VEITVTAEGAPDVVVLAEVTDRTISAASAPSTSSAPSVSIPA"
     gene            complement(25143..26144)
                     /locus_tag="VN041_13085"
     CDS             complement(25143..26144)
                     /locus_tag="VN041_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU30009.1"
                     /translation="MINRPAAFGWAILGTGTVSHSVAADLSIVDGVRRVGVWSRSAKK
                     AASFATTHRFERAFTSIEDVLAAPDIDIVYIATPHATHARLAIASLEAGKHVLVEKPI
                     GVNADEARAIARAAEANDRFAMEAMWMRFAPAYIALREDIAAGALGDISGVRAAFGLP
                     FGHPESERWTSELRSSTLLDQAIYAVTLARDVLGEPLNVTSEATVRADGVDLTLHATL
                     GYAGGRFAQLAASMVTYLEPTAAVSGTNGWATIAPPFWATDRYRLHAGDLGEAFGEPT
                     DRVFPQQGFGYVPMLRAVHEALSEGATQHPHHPLSDSVAVLQTLDTIRSEWTATAAA"
     gene            complement(26141..28414)
                     /locus_tag="VN041_13090"
     CDS             complement(26141..28414)
                     /locus_tag="VN041_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU30010.1"
                     /translation="MTVLPYRDPNLPIHERVEDLLSRLTVEEKAGQLTQYFYTGDGQP
                     IPEDFDIDSLPPEHRIHVELPALIEAAIRSGRAGSLLFLRDPALSNRLQKYAVEESRL
                     GIPLLFGFDVVHGWRTIFPAPIALAATWSEQTVEAAQAAAAREARAAGVHWTFAPMID
                     IARDPRWGRIIEGAGEDPVLASAMSAAQVRGFQGDLGTDRLLAGPKHFVGYGASRGGR
                     DYDDAEVSDHELWNVYIPPFRAAIDAGAGNVMSAYMDLNGVPAAASRTLLTDILREQL
                     GFDGFVVSDANAVRSLSVQHFAQDHADAAARALDAGLDMEMCMYEPAFDSLPQALAEG
                     MVTESVLDSAVRRVLTAKFRLGLFENPYADEASTAAVITDPENRILARSVAEASMVLL
                     KNEDAVLPLDASAVGRIAVIGQLADSRRDLLGPWVFDHDTSETVTIVDGLRTALGSGG
                     VSFEPGAGIPERIYPSMFDVMDATVERTPEGYDDDAAIERAVALAEDSDVTIVVVGQR
                     QNQIGEKSSTSTLDLPGRQLEQLQRIQATGTKVILVIVSGRPLDLRWADENVPAILQA
                     WYPGTRAGEAVASVLLGGTSPAGRLPFTWPRHVGQVPLIYAHNRTFLPEEKDARYYEE
                     SNAPLYSFGYGLSYSTFEYSEISVERSEIARGEATTVSVTVRNTGDRDADDVVQLYIH
                     QRHGTSTRPVRELKGFRRISLAAGEARRLEFKLGPEQLQYWSAVTSSTIQDATIFDVA
                     IGGDSTAPFTETLTVTP"
     gene            complement(28473..31013)
                     /locus_tag="VN041_13095"
     CDS             complement(28473..31013)
                     /locus_tag="VN041_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HWU30011.1"
                     /translation="MESRQFDCRSSLSRSADPMSESRSARFVSPPFAQGAVGEHASYL
                     RKEFVASSVPRRATLRSTAVGLIEPHLNGSVVGDEVLAPGWTSYRHRLIVSTHDVTDR
                     ITAGANVIAAVLGEGWAVGRLGWEDRRHHYSDRPAAWFELDLEYADGSRDTIASDTST
                     TASRGGVGANSIYDGETYDARDEPEGWDRPGFDDSGWVPVETFEWPLEALTTPIAPPI
                     RRIEELAPADLMRTPSGKLVLDFGQNISGWVRFTVQGDSGTIITLRHAEVMVDGEIEV
                     ETLRTAAATDQYILRGGPAETWEPRFTFHGFRYVEVDGWPGELSADAIRAIVVHSDMA
                     RTGWLTTSNPQLNQLHSNAVWSMRDNFVGVPTDCPQRDERLGWTGDINAFAPTAAFLY
                     DVRGVLGSWLKDLAVEQQEKGFVPWVVPDVLSTPSSPTALWSDVAVSLPWALYREYGD
                     EKILEDAYASMTTFIRQVADLLDDDGLWSTGFQYGDWLDPDAPEDNPAGGKTDRHLVA
                     GAYLCRTTREMADTARVLGHRADAAEFEALAERVRDAFRREYVSPNGRLVNETATAYA
                     LAISFGILDEAQRQHAGDRLAAVVARAGYRISTGFAGTPLVTDALSSTGHLDEAYLLL
                     LETKAPSFLYPVTMGATTIWERWDSVRPDGTINPSGMTSLNHYALGAVVDWMHRTIGG
                     LEATTPGYRRMRIAPQPGGNLTHADLTHDTVHGQVRIGWRLEGSQVCLDVSIPECTTA
                     EVVLPLHPDSAVIEIRGGEHSWQYDVAPVADRAPLSLDSTLREVSDDPAGWHRFTEAF
                     ARHYPGVPLDGKAPEAAGMSIRTMLDYVPGASPELVDELQSALEGPAR"
     gene            31152..32162
                     /locus_tag="VN041_13100"
     CDS             31152..32162
                     /locus_tag="VN041_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30012.1"
                     /translation="MASQTSGRRVTAADIARSLGVSRATVGFVLNETPGQTISAATTE
                     RVLAEARRLGYRPHIAARALASGRSHIVLLVLPDWPIEHSLRENIDEATHALDEHGYA
                     LITYTPHEGSRARPLWETLQPDVVMGLLPFPDALIESMRDAGVERILPDPDEVTDVRY
                     NEGGPRLQVEHLHDLGHRKLGFARPSDPRLVELADAREEAATAAAVALGLDVPATFEL
                     APAASEADPPVTEWVESGITGVVAFNDDVAAAIIGAAARAGIAVPEKLSVIGHDDTPV
                     SRLFEPRISSVRIDSTGLGQYLAALAMHAMDQTPTPDWEQTSAVTLIARASTSVSPIA
                     QG"
     gene            complement(32223..33620)
                     /locus_tag="VN041_13105"
     CDS             complement(32223..33620)
                     /locus_tag="VN041_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30013.1"
                     /translation="MCPCRHYLDNGVLMTTPNVSGSEADSPRAELQSPLPAAPGAIQE
                     GIRTTTGLIRPARASRSYIWLLVLAQFGIFVAFITPIAISLAVRVSALAPGHDEYLGY
                     ITGAGSVVVMIITPIVGILSDRTRSRLGRRRPWILGGMLVGVLSLFVMGLAPSVILLG
                     VGWILAQIGWGQSLANLTTSMADTLPEHQRGKVAGFVGFATQVAPVAGVIIAGSLVGD
                     PLLLFLIPGIVGVVFVTLFLLFSREQDSRQMIVDGVLTIGTTFRKYLYNPAKYPDFSL
                     VWAGRFCFYFGLTLNTTFTAFFFAARLNISVEEIAGVLATLSLVGIAATTIGAIGGGF
                     LSDKLRRRRLFVLLASAIFAGGVVVEAVASDITMLFIGSLIASVGIGAFSAVDQALLL
                     DVLPERDTDAGRFMGIAGFATAIPQAIAPFLAPLVLAIGASATGDKNYTLLYFVAGAI
                     TVVGGLLVMRVRQVR"
     gene            complement(34224..34700)
                     /gene="soxR"
                     /locus_tag="VN041_13110"
     CDS             complement(34224..34700)
                     /gene="soxR"
                     /locus_tag="VN041_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HWU30014.1"
                     /translation="MPQLDGDKSRQLTIGELSRRSGIATSALHFYERNGLIVSTRTAG
                     NQRRYGRDTLRRVAFIKVSQRVGIPLKDIRSALESLPEGRTPTKRDWARLSQRWRDEL
                     NARIRQLEHLRSDLDGCIGCGCLSLNSCALQNPGDVLGREGSGPIRWLKKDRPTVE"
     gene            34758..36176
                     /locus_tag="VN041_13115"
     CDS             34758..36176
                     /locus_tag="VN041_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30015.1"
                     /translation="MTADTREAGTANAAAPGILSRTYLWITMGACALVFLGAFESLAV
                     TTVMPAVSADLDGERLYALAFAGPLATGVIGMVAAGIWADRRGPVLPLYSSVIVFVAG
                     LLVAGLAPSMEVLVAARFAQGLGSGALTVALYVVVGRVYPQELHPAIFAGFAAAWVVP
                     SLIGPTVAGAVTEIWSWHWVFLGVVVLVFAALLMVVPALRGLTHGGDDTVPWAFGRLG
                     WSVLAAAAVLASSLLVDVPGIGSLLAGAAVIVALVAVRPLVPRGTLRARRGLPSVILV
                     RGLSAAAFFGAQVYLPYLLTDSYEFTPTLAGLSLTGGALAWSCAATLQGRMTATLSSI
                     TAVRIGTILVLSGTLLAAATAAFHWDAAIIMVSWVVAGTGMGLMNPRSSALTLSMSTP
                     ENQGFNSAAMTVSDSFGSALALTITGAVFAVTAAAMDPFTAVLVLTVAIGVAAVVVAP
                     RVRAGATGVSAPEKRGSRTPPR"
     gene            complement(36309..36749)
                     /locus_tag="VN041_13120"
     CDS             complement(36309..36749)
                     /locus_tag="VN041_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU30016.1"
                     /translation="MDAEAVSPLHVALQLHRAYAIATLQLNKALKPLGLSYRHVAAMF
                     LIRDGVRTHRDLTYELQTDKAGMFRTLIDMEGQGLVTKTRSERDRRSQLFQLTKHGHA
                     TLVMAQRHTRTVAENLFGTLSSDELATVHTALGRVQGPHHESSA"
     gene            36808..37257
                     /locus_tag="VN041_13125"
     CDS             36808..37257
                     /locus_tag="VN041_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30017.1"
                     /translation="MSTDDRPVPVWPRMGLRASSCIAAGLLALQSLTAGLFLAGVEAA
                     FDVHRETATAAGIAIMVGIVFGVMSVRLRGESWKPVMWSVGLLALMSLQAFAGFRSLT
                     ALHVPLGVLTIFAGVMVTAVSWGSPFGRAEGRTSADGSGGRRSRRNK"
     gene            complement(37383..38630)
                     /locus_tag="VN041_13130"
     CDS             complement(37383..38630)
                     /locus_tag="VN041_13130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30018.1"
                     /translation="MRSNWTRRRGGASWVVDAYTLPLASLLLFGGSLGDRIGAQRLFK
                     IGALGFAMASVGCALSPSLAVLIVCRAAQGVFAALLLPMILALVGKSFDDPAHRATAV
                     NLLAVFGGSGMAIGPFLGGLLTDTASWRAVFWLTVPIAVTATVLAGHTDGPGDSSRRL
                     HVDLPGQLTGTAGLVALVAGLIEAGSASSPFVSWGLISLGALLLCAFVAIEHRVTMPM
                     MPLSVFRAPGFPGAVLGGFALQFGAYGLQFFLAVYLQAAWGVTAFTGGLLLASFAIGV
                     VLAGVVINPALQRRGARAMTLTGATVAGIGTLALLLVSGGHMWWLLLLAEFVIGIGTG
                     IYSTALNQTASCSLGYAAAGLGSGIYNTARQVGQSIGIAILGSFAHLTNQSLGFSMAI
                     ILVTGCAAAIVVTELRPRTTPGR"
     gene            complement(38748..39311)
                     /locus_tag="VN041_13135"
     CDS             complement(38748..39311)
                     /locus_tag="VN041_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30019.1"
                     /translation="MSTRDDLIEATRELLWERGYGATSPRAILDASGAGQGSMYHHFR
                     GKESLARAAIERNADEMRSQIASDLASAPSAVGQVRAYLTREREVLKGCQFGMLAQDA
                     DILESPALWAEVRDMLAWVRSQLAEVIQRGVDAGELRADLDAGRTGTAIAAILQGAYV
                     LARAAQDTSAFDDAVEGALALLTAARA"
     gene            39442..39747
                     /locus_tag="VN041_13140"
     CDS             39442..39747
                     /locus_tag="VN041_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30020.1"
                     /translation="MAAGDWVKVDAWLVAPSEAGEYVRRREALALAVLAEAERRGLRA
                     GRAGKGSEDGEYVIASDAAGETAALVHLDPMTTDEWAQASARDRLTDFFARHFAASA"
     gene            39902..41116
                     /locus_tag="VN041_13145"
     CDS             39902..41116
                     /locus_tag="VN041_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU30021.1"
                     /translation="MSKAIIIGAGTAGPAVAQLLTQQGWEAPVFEARSSLDPAEGLFL
                     NVAVNGRRVLQRLGVVDRLLSDGHPAGVMEMYSGKGRLLATVPNGPAGKPASGGVVVR
                     RGWLQQVLWESAAEAGVHIHTGRRVVRVDESTRGVRVHFDDGAVEEGDIVIGADGVGS
                     RVRRFIDPGVQAEFTGLIGAGGLARVPDLDPTPGVQRFVFGRRSFFGYLVREDGTVYW
                     FANATASEPPPQDHPADAADVLAQLRALHRDDPDPVPAILQNTVGPVGSYPIFRLPTV
                     PRWWKGRAVAIGDAVHATSPSAGQGASLALEDAASLAAALSHNPDHERAFASFQADRQ
                     QRAEKVVAYAAEIDRQKKTSGNHLAVAFRDLLLPLFLRNATKDTRYDWVFDYHLPTMK
                     HSDNQPGGARDQ"
     gene            41211..41567
                     /locus_tag="VN041_13150"
     CDS             41211..41567
                     /locus_tag="VN041_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30022.1"
                     /translation="MVFAIFAAVHALLVLAQPFTAGWSLEFGGGALDWHGNTANAIVT
                     LGFLQIIPGVLARWPGGAPVPAPITAVLIFLAEVMQLQLGYHGGFALHLPLGVAVVGG
                     AVVLAVLSFRWARPRP"
     gene            41747..43090
                     /locus_tag="VN041_13155"
     CDS             41747..43090
                     /locus_tag="VN041_13155"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWU30023.1"
                     /translation="MQDGARRFELVAQAGKSRIIDAGSTTTWGINGPLLGPTLRVRRG
                     DHIEMAVRNDLGEPTTLHWHGMHLPAAADGGPHQTIAPGETWSPNWVVDQPAATLWYH
                     PHPHGQTERHVYRGLAGMLIIDDDNERELGLPRRYGEDDFPLIIQDKTFNKSGELLET
                     QRYDNGMLGDTILVNGAVSPILTITAEHTRLRVLNASTARSYNIGFSDDREFTMIGSD
                     GGLLRAPVALTRLHLTPGERAEIILRCAPDDELTLRSYPHPQGLPTAIADATGASDRL
                     DILLIRAATALTPSSPLPDELAVLPALEPPPESTWRRFDLRGFRINGADMDPSRIDVT
                     VEVDTTELWEVRNAHDKPHNFHIHDVQFQIRTIDGQAPPAHLAGWKDTVYLPPRTPVQ
                     LLMRFSDHTDRNMPYMYHCHLLWHEDNGMMGQFVVVDPGETAGTPPPIRGDHGGH"
     gene            43158..43661
                     /locus_tag="VN041_13160"
     CDS             43158..43661
                     /locus_tag="VN041_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU30024.1"
                     /translation="MDEAGNVDDEDLPDRNEEIALHGAAMQEFMARAVLFQDAVAHAA
                     GLNSTDLQTIGILMSNGPLTAGELAARVGITKGGAITAVIDRLERSGYVRRSRDLEDR
                     RRVAISAVPDALMPRIGPIYARISQIWGEYLDTLTTDQIRFATDMLRQAAELNATETL
                     RLRADHP"
     gene            43752..44393
                     /locus_tag="VN041_13165"
     CDS             43752..44393
                     /locus_tag="VN041_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWU30025.1"
                     /translation="MPSPEALLAFALASVILIAIPGPSVLFVIGRSLSLGRSGGLWSV
                     LGNELGALAPITAVALGVGTIVAQSVMVFTIMKVLGAAYLAYLGIQAIRHRRDGIGGV
                     EPATPSRVSARVVLRQGFIVGATNPKTIVFFVAALPQFVDFHAGAVPMQMLILGLVFT
                     FIAFICDAVWALLAGSARAWFARSPRRLAAVRGTGGGMMIGLGGVLLLADNKA"
     gene            complement(44786..45358)
                     /locus_tag="VN041_13170"
     CDS             complement(44786..45358)
                     /locus_tag="VN041_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWU30026.1"
                     /translation="MARFTAFLDACVIVPLAPCDTMLRMADAGAFRPLWSQKVLDEAL
                     SALERIHPDVDPSRFRSRFRSMNEAFDDALVEGWEPLVAGIELPDPDDAHVVAAALLG
                     RADVIVTENVKDFPDDVLAPLGLEVIRTDEFLLDQFDLSPGATCRIVTEQSAAMRRPP
                     VEISQLLERLARSGAPNFAQRVAAELQRDS"
     gene            complement(45361..45903)
                     /locus_tag="VN041_13175"
     CDS             complement(45361..45903)
                     /locus_tag="VN041_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HWU30027.1"
                     /translation="MDDLNDLGDNSHMTATAARTKDPLVVDPPQDLSEMLELARFLEN
                     HAAPAMLLGPDGEQIPLPMEAYDVLRQVVSAMERGEAVSVEPIDRQLTTQQAATLLGV
                     SRSTLVRLLDEDELPFERLGESRHRRLRLHDVLAYRDRKRADRRIRLDELTRQADEDG
                     LFDVDADAYRDALAAARKQS"
     gene            45979..46224
                     /locus_tag="VN041_13180"
     CDS             45979..46224
                     /locus_tag="VN041_13180"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HWU30028.1"
                     /translation="MRTTFDAEANAAYIQLVPEITPGRSVRNEIVETAGGTVVLDFDR
                     AGVLLGVEVLGAEKLLDGEFLAGAARIDQPSGSPSSR"
     gene            complement(46259..47443)
                     /locus_tag="VN041_13185"
     CDS             complement(46259..47443)
                     /locus_tag="VN041_13185"
                     /inference="COORDINATES: protein motif:HMM:NF017832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HWU30029.1"
                     /translation="MPLSATYRGERVESWSMSEDEWTQLKTGYRAAGLTMMCGQQGVP
                     KTSKLGTRFFAHKPGTDCLLHEGGPESQEHLATKAVVARAAREIGWDATVECPAPDRS
                     WIADVLVSNGNRRLAIEAQWSPQTDADFERRQKRYEATGLERVWLAGPRNVISAGAVP
                     HYAIDGSVGSLEMQLPILHGSQSFDLADGIKRLLGGGIRPIAELIVTRAAVQTQMVKC
                     WHCSKWGTLWTVTALELESRCGQTATLEWSTPWELHAAERHEKALEEHVLRELRAADL
                     PPAVVLQMRRTEAAGVTYLAMICRSCNRLQGDGMLPEGFRAGRSGSEYTVPLGKGVRL
                     PFADWVVERAHVCEDIGRGRCVLPSSTTAATTDSAVYPPATEHVYFTGWRMDAPALPQ
                     RS"
     gene            complement(47528..48292)
                     /locus_tag="VN041_13190"
     CDS             complement(47528..48292)
                     /locus_tag="VN041_13190"
                     /inference="COORDINATES: protein motif:HMM:NF027453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4839 domain-containing protein"
                     /protein_id="HWU30030.1"
                     /translation="MTDADARPEAETAKYETRNVQTVRGTEKLMISKWEKDGWELVTQ
                     NAGKLRTELVFRRPKPKVQWKLFAIIGGVLVVLGIFIGIMAAITGGDKDGDAGPASTP
                     SAEAVAPRDEPSTKPEEPVATAPAEETPLTAENNADLAALLTGPQDGPTVEAFATQYS
                     GQLIEFDGSIGAMNKHEGYTTRYDIMIVNGDYSETHSNGGPSFQFRDVNITNDLHLTG
                     DVPDTIGVGTNVHVIARVGSIDGQLFLLEPVQTSVR"
     gene            48548..49393
                     /locus_tag="VN041_13195"
     CDS             48548..49393
                     /locus_tag="VN041_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU30031.1"
                     /translation="MSSKDEAREFLTSRRAHVTPAEAGVTDWGEERRVPGLRREEVAD
                     LAGVSVGYYTRLERGEIGGASESVLEAVARALRLTEVEREHLFDLARRASSSSRSTAR
                     HPSSSVLEPMRRLVESIGVPAFVQTPRLDAVAANGLGRALFAPVFEASEPNFVRFNYL
                     DPRARDFYLDWPLARRTGAAILRREAGRDPLDGQLTALIGELSTLSPLFRDDWARTDV
                     HEHRTGVKCFVHPEVGRLDLTFQVLETPGDPTHRLVTYSAEPGSDAADKLAILGSLAA
                     ERHGE"
     gene            49474..50448
                     /locus_tag="VN041_13200"
     CDS             49474..50448
                     /locus_tag="VN041_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWU30032.1"
                     /translation="MKIVVIGGSGHIGTFLVPRLVRAGHEVVSMTRSGRNRYAASTEW
                     DQVTHVAVDRDQQDTKGIFGRTVLAQRPDAVIDLLCFTIESATALVEALRGNVGHLLH
                     CGSLFRYGPTVKYPIREGGGTEAIDEYGRQKEAIARMLIAESASPGGLATTSIHPGHI
                     VGPGWLPINPLGNTDVSVWHRISSGIPLRVPSQGGDTMHHVHADDVAQLFQLAVENRQ
                     ASTGEDFHAVAPSALTVRGYSGIVGGWFGQPGEIEPITWEQYRAETTEAFAQHSWDHL
                     HRSHYLSIDKAKSLLGYAPAYEPEQAVLESVRWLIENDQLDVARPLSV"
     gene            50680..51537
                     /locus_tag="VN041_13205"
     CDS             50680..51537
                     /locus_tag="VN041_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWU30033.1"
                     /translation="MITFTDFLPVPEPQLTKVKFNMRSGDGSGEALDFLMDDHERWLN
                     FGRWRTKQTNNNMGGARYLLAFAQYYPYGPQYFIFGGAYEVTEIKPDRIDDHGYELTP
                     LPLHEDFIKRLIIKLERPIGRTPYLRHYVGLQESTLNPEVYELAPDIKLGTFPGYQNV
                     RLRHHELQRIIAGDEPSWRDALSSVKGVYVITDLSCGRLYVGSASGEANGLWQRWSGY
                     AHLKNLTGGNVELEQLRRNLGDAHIVENFQYSILEIFDPKTRADTILARESFWKNALD
                     SRAHGMNLN"
     gene            51639..51917
                     /locus_tag="VN041_13210"
     CDS             51639..51917
                     /locus_tag="VN041_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30034.1"
                     /translation="MLIAVAGLMLFHTALIITVRANRRSRVPFWRNAEKIPAGTVAMR
                     VIGAGFIVLGIVLLGVNAWWVPFAIVLAGPVVALMVIAIHNRSVATAR"
     gene            52058..52822
                     /locus_tag="VN041_13215"
     CDS             52058..52822
                     /locus_tag="VN041_13215"
                     /inference="COORDINATES: protein motif:HMM:NF021057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HWU30035.1"
                     /translation="MTETRPAISIEGTAFEIAVIETMTPAEFGIALITAIHCGGRKRE
                     WSFADLVTVIESRRPVARRVDGVRIDDEYSRLDIRQRVADGAGWLISRGLIGPASNSS
                     GSTGEWRLTTAGIEALESGDVRIIEASYRLPLSLHPRLGEARALFERGSLQTAVFSAT
                     HAVEVRVRELAGYGPEKYGTGLMQEAFNDKTGPLAIGDTPAERTATMQLFLGTVGAFK
                     NPTSHRAVEYDDPSEAADIVQLCDLLLKVAERSASV"
     gene            53019..56528
                     /locus_tag="VN041_13220"
     CDS             53019..56528
                     /locus_tag="VN041_13220"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HWU30036.1"
                     /translation="MSDATSVGGAANELGSRHRAGVATYLAVHGLVDREVLGRPGAVP
                     IAISLEAAEAVDDIVCRMADDTRWFSQSKRTAGNSEPFRAAVRQWAQQQLEHGDRVVL
                     VARELRGLLSGLQPSIDQVVRGDPVPDNARTKVEQFVGVLRDDGVADPELLLGRVQLI
                     EWRVETPGDPDQEAGIAMLADSIVPFNQAPRAFEVLRARMLLAATRRETTTVGDWIDC
                     LVAADLDVYADGAGPRGARERARQLATASYRAHLARRANRLDLSALVTDVPEIIVEDA
                     LDAWEVSWDASGPRQIDKTDVLNVIRATPRVVIVGLPGMGKSELMRQLAARLAQEPLA
                     PVPIHIDLRNIASAVRSADDLTLDLLLELPSRSVTGQDAGVLKSALTDAVTRGDAILQ
                     IDGLDEAQRARGQVAAGLARLLAELPPATGLTLTTRDSALASAEQLHLPLVRLEPPGN
                     LRDAMLTLITELAPQHGAADSTEWIAGKNDELIAAFGSHKDVWSVPLLATLATVRLAS
                     GRGEAESVAVLLNQVIEDSVSEWELRRAAGRDELHTAVDSPMLLEGFAAIGHELVGRA
                     SISRAEAATAVRAALAAWHLAPPVEGTVAAQIVNFWDERVGVFVDNGDAIVPRSRQFA
                     ELAEVRWLSDRTKAHRSEWLQNAVQDDSYENAVSLATILHADVRAALIHRASRVSADP
                     SRDRAATWVARQWRTWPAADQSQQLQIIDVLADAAEDQLPSPGQSSGILRAMDANRRR
                     TDGDGWEFVLALVNAPRIPGVDDRRRERLANLDVDLERRTMVTALDGLIDASAEGRAP
                     DAETLAAVEEILSVQVPAEPGSQYNEEGVLVIKSSPSFVTGVGDIAERCIEFLDRLPL
                     EAPDAVYAIARKQSIRQYARINRRLVALGHRDPQPAAAASRLLDMLEAFDDDHGGLAW
                     LLRALVQLSPDIGSEPGASWRYPNVGRLIDALGYAETTIQGLARAAEEDANIVQGWLV
                     AHIEAFGLDVAVVAAEADQLIDADDSKAASDSIIYGRRQSEEDPATLTEPTALALVPA
                     LRSRSEWIVNSSFRLLTQTRYPSVATAVAEIPGRTSWRTRFERNIAIVYNAADAEAAV
                     VRMVASENTAVRSAAAAMLALDYVPRSDLLDGLRDDGDGQVRYYAGGEPRNATEWTCS
                     SCAEVNTTNHTSCSRCHVTAAWR"
     gene            complement(56871..57022)
                     /locus_tag="VN041_13225"
                     /pseudo
     CDS             complement(56871..57022)
                     /locus_tag="VN041_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830349.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10240 a        18223 c        18373 g        10186 t
ORIGIN      
        1 gtcgccagag gagacgccgc aggcggcaag ccccagcgtg gcggccgcta gggacgctgc
       61 gagaggcttt cgcagcgaat gtgtgatagc tgtcatgggt acctgtctcc ttcgttgttg
      121 ttcgctgtca tccatctgtc ggggtgaggc cgcaacctcc cccatgccct cactgcagcc
      181 gtatgcgcgt cgcgccgagg tctgcgtcga agaccaggct ggacggccag ggcttccatg
      241 tctggcccag ggccgcgttc tgcgggtcgc ccgtctgcat gaaggccttg aggctggcca
      301 tcatggcgtt cgacagcgcc agccggcccg gggcattggc cgtgctgttg gtcacgcggg
      361 agaacacgga cggcccgaag ttgccgaaga gaaagggcag atcgaacgca tgggccgcac
      421 cgtagaccgt gttccagggc gcgggctggc gcgcccagtc gaagcgatag ctccagacct
      481 cggcctgccg ggtgcgcagc gtgttgagca actggtcgcg gctgggatcg gcgaacagat
      541 gggtcaattg cgccatgcgg gcgttgtagc cggtgaccgg tgtatcgacg ggcaggtagg
      601 cggcatcgat gatgtcggcc aggcccggcg cgttgggcga ctgcgcgtcg aaggactgca
      661 tcatgcgaaa gcgcgccgca tcgtcgatcc tgaagcccgg cttgccgccc agcgcagcca
      721 gcaggggcgc gaacagcacc agcagcgccc cggtggccgc ggcggagagc gcaaaccccg
      781 gtgaccccgc gaaccaggga tcggcgatca gcagcccgca cacggccagg gacagcatcg
      841 cgagaccggc gctgggccgc ccgagcagga tgctgagcat cgcgagcgcg gccatcaccg
      901 cggcacgcac cacgctcggt tccggcgtca ccagcaccac gaacgcgccc agagccagca
      961 gcgcgagcac gacgcgcagc gtgcgcccac cgccggcgag ggagacgagc cagaagaccg
     1021 cggccacgat gatcatgcag ttcgctccag atatagtcac ttatggaggt ggctatggcc
     1081 cagctgatcg ttcgcgagta tctgagagtc tcaaaggacc ccagaggcac cgggaaaagc
     1141 ccagatcagc agcacgtcga gaacgtcagg tcgttcgagc aacagggctg ggagctgcac
     1201 ccttctgcac cgtatcgaga caccgatcgc agcgccagcc ggtatgccaa gcgcaaccgg
     1261 gaggacttcc ggcgccttat cgccgatctc gaggatgaca cgttcggcgc cgatgtgctt
     1321 gcgatctggg aatcgagccg ggggtcccgc cgtgtcggcg aatgggttga cctcattgac
     1381 ctctgcaaag aacgccaggc gcgaatctgg gtcaccactc acggtcgctt gtatgacccc
     1441 gccaacgcgc gcgatcggcg cacgctcctc gaggatgcag tcgacgccga gtacgagtct
     1501 gacaagacct cagagcgcat ccggcgcaac gtccgagccg ccgctgaggc tgggcggccg
     1561 cacggcaaga acgtgttcgg ttatcagcgc atgtacgacg cgaagacccg cgagttgctt
     1621 cgtgtcgagg agcaccccga acaggcagcg atcgtgcgtg aggcagccag aagggtactc
     1681 gccggcgaga cgttctatgc gatcgcgaag gacttcaacg tacgcggtat cccccctcgc
     1741 cgtcccacgc gagttgaaca ccgcaagaac cacggttgga cacctcctgc aatcaagcag
     1801 atgctgacta tgccggccta tgcgggtaag cgccagcacc agggcatgat cgtcagcgtt
     1861 gcattgtggc cagctctgat cgactacgcg acgtgggaga agttgcaggc ggtgatgtcc
     1921 ccgcagcaga ggagacggac gaacgactgg ccagcgaagc acctgttgtc gggaatcgcg
     1981 ctgtgcggtg tctgcggaac tccgatgcgc gtcggcaagc agaacgcggg ctcgaagaag
     2041 tttgacgagg acgggaaccc actccctcgc gaggtgtacg gagcttacgt atgctcgggg
     2101 atccctggtc ggcccggccc tgatgggaaa cgaggcttcc acgttgccat gcgccaagag
     2161 cacctggagg aagtagtgat cgagcttctc tttgcccgct tggagcgccc cgacttcctg
     2221 accatgatcg gtgatcgcgg agacgcagca ggtgccgagc gagatgcatt gctcgcggag
     2281 atcgctggtt accactcgta tctggaccgg ttgcgcgaag aggccacagc gctgcttcga
     2341 ttcgacatcc ttctggatca ggaggctcga atcgagccaa agatcaaggc ggcccagacg
     2401 aagctcgaga agctgtcgga gatggacccc ttcgtcctga cgctgttgtc aacaggagca
     2461 atccgcatgc agtgggactc cctaaagctc gcggaacaga ggcgcgtgat ccgggctgtg
     2521 atggccccgc gagtcaaccg catcgggaaa ggctggcgcg gtcgcaaggg cctcaactac
     2581 gagcgagttc agcccggctg gcgctagttc agggcgtcgg cactctgcag ggattggttg
     2641 agctcggaga ggatgggtgg gcgcgaggtc gcaacgccat gcccattctc tgttgggccg
     2701 cctgagctca gtgcactgct gtagcgactc aggtcccatt cgctacagct cgctatgcaa
     2761 gacaccgcgt gcccagggtg atcggagggt gttgtagcag tgtagcgacg tagcagcagt
     2821 aaataggctg tagagctcgt ggacgaattt ggaaggtcta cagcctgagc gcgccccact
     2881 gctacatggc tacatcgcta catcctccgc gtgccgtgta atcacgcgct cgccatgtag
     2941 cgatcggacg accctattgc gctaccagcg tgctacatgc cgagagaact cggttgcgcg
     3001 tcgagcactt gctctacacc cacgaacagc gattgatcgc cgttaatcag aaatgtccgt
     3061 gggtgctgat aatgttgagc acactcggca acccgccggg tgcacaactc cacatctata
     3121 ccgggagccg gcaggcgaca cggtgaagta cacatgcgac tccttcggga gtgcggatag
     3181 cagcgtttgg ctgacgtgtt gtccaggacg acgagcccca catgaagaag gaccctgact
     3241 tcagggtgcg gaccaccgct ttggctgagc tgtgatccag acagactagc cccgaacatc
     3301 gaattgattt gacgttgtgc tcctacttca caggagcatt cgatgcctct cgcatccaca
     3361 actctcattg atctcccgac ggcagcgacg cgcagcgact acggcctctc caccctccgg
     3421 cgccacatcc gggccggcag tattccggcg gtgaaggtca agggccgcat ctacgtgcga
     3481 cccgaagacc tcgacgcctt cgcagcgccg gagccggtcc atgtgccgga cgtcacactg
     3541 caggaatggg ccggacggat ggccgccaag gcgccggcgt tccgacccga gcagcgaaac
     3601 gtcatcatgt ccgccttcgc ttcggcgctg ggcggtgagt gattccgtga cagcaaggga
     3661 aagccgccca gcggctaacg gggcggctct cgaagagttt cacggctcta ccgaggagct
     3721 taacaccgcg actcagcaga caacacaaca gaccgtccgg actacgccgg cttcgtttgt
     3781 gccgaccacc caggagagcc tcgtcgagcg aaccgtcacg aggtacctca tggagatcgt
     3841 tgatgatccc aatatcagcc ttcgcaccgt gcgggacacc ctcattagga ggatcaacgc
     3901 ggagttcgat ctcgaaaacc ggggacgcaa ggacagcaat tcctacttgc ccgcgattaa
     3961 gaagacaccc gttcttgacg agcacaccgt cacctcggtg ctgctcgctc gccaccgcgt
     4021 tgtcgcaatc gacctggcag agggtgaggc cgaggatttg actgagctcg ccgtgtacgt
     4081 cgattttggc gaagctgcgg gtacctactc gacgtcaaag acgcgcatta ggagtctgct
     4141 gagcgatctg aggccgtcga tgtcggcaaa cgcgatcgag tccgcctatg cacggctgag
     4201 gatccacgcc ccggtagtca ccagaacgat tgagcctcat ctcattcctg tggcgaacgg
     4261 tgtcttcgat cattctcggc aagttctccg tgagttctca ccggattggg tcttcctctc
     4321 gaagctgccc gtcgactacg acccgggtgc aaccaacgag cacatcacga tgccagatgg
     4381 cgacgactgg gacgtcgaat cgtggcttca gagtctcagc gacgacgaag gggttcctga
     4441 gcttctttgg gagatcatca gcgccgcggt acgctcgcac gccccttgga accgcgccat
     4501 ctggttcgtg tcagagcggg gaaacaacgg caagggaacg ctgatccagc ttctccgcaa
     4561 cctcatcgga accaaggcat gctcttcgat cgccttcgcg gattttggaa gagagttcct
     4621 cctcgagccg ctcctctact cacgagtcaa ccttgtcgat gagaacgacg tgggaaaact
     4681 ggcggagagc atagccgcct ggaaggcttt cgtcacaggc gaccccatca cgctcaaccg
     4741 gaagcaccgg acaccggtga agattcgttg gcgcggcctc gacattcagt gtcacaactc
     4801 gacgattcag aggaccaagg accgctcggg gtcgttctat cggcggctcc tgatcgttcc
     4861 cttcaacaag tggttcggcg acagtgagcg cgcatacatc aagaccgact acctcacgcg
     4921 cgctggcgtg ttgaggtacg tcttgaagcg agcccttgag atgccccaca gttcgctgtc
     4981 cacccccgag gcgtgccgac gagctcttga tcagtaccag ggggacaaca actacttgtt
     5041 ggcgttctgg ctggagttca aggaccagtt cgtgtgggat ctgattcctt accgcttcct
     5101 tcacgccctc tacgtcgagt ggctgcgccg ggtgaacccc agcggtcatc ccgagagtca
     5161 agccgcgctt actgcgctct tgtcggcgca tctggcgggg tctgacgagt gggacgcgaa
     5221 gcgttcggat acccgtccag gaaccgcaat ggcgctggca gagcctctga ttgaccagta
     5281 cgggctgacg gattggatga atcacgatta caccggcact gaccgttcta agcgctgcat
     5341 cgtgagcccg ctcaggcaga actacaaagg cctgattcgc cgcacgaaga gcaaccactc
     5401 tgccgcgggg gcagcctcac ccgtgacgag caccgacgac gactgacctt ccgctgactc
     5461 ccaatcccga gcccagttgt gggctgaccc cgagaaccga cgagagaaga gcctcgatgc
     5521 gaagaacccg tagtcagatc cgcgccgccc agattgccgc gctggtcgcc gagcaggagg
     5581 cgttcgacag gagcgtgagt gaggcggtga aggtcgcctc attcgcacgc tgcaacgccg
     5641 tggaacagct ctatgaactg ctcgaagtga aacccgagaa gtcgatcact cgtgcgggca
     5701 agaacggtgc gtaccaggtt gcgacggaca aggacgagac gaagcgcagc gcccgcttgg
     5761 ttgaggcagt tgcggtgctc gtgtcgcagc ttgactcgcc gaactccgcg ccgttcccga
     5821 tgacatcgag acgcggatcg cgcgcctcgt tgaccacggc ctcccacctc gaggcacggt
     5881 gtagcggtgc cagtcgtggt ggcgagctca acgcgttcag cggacgcctt gatcagctat
     5941 gcgctcgaag acaagcccga ccagaggggc gagcgctacg tgatggcctc cggtgttggc
     6001 ggcatgctgg tgtcggtcgc gaagcaccag atgcgcgacg tccggaggaa atgggccaag
     6061 gacaaaccag gcgctttcgt gcaggcgtat cacgtcattc agagcttcgc gagagacgaa
     6121 ctcgaccccg agcagccaga ctcatggatg acagcgcaga agctgggtcg agctcttgcc
     6181 gaggaacgat tcccgggacg ccaagtcctg gtcgtgacgc agcgagatgg ccgcactgga
     6241 tgtgttcaca accacctcgt tgcgaactcg atcgagacta agtctggccg gagtctcaac
     6301 agcgcagtcg tcactcacgc acgactggtt gaggcgcacg aacgagtgct cgacaagcag
     6361 ggattcgcgc agcgtgcgga cctgaaacaa gccttcagtg acgccaagga gcgtcaggag
     6421 cgcggcgaac cgagcagcct tcgtcgcgcc ggctcaaacg cgaacagcga gctccgagag
     6481 ttccaacagc acatcctctg ggagaccgac tgcgacctcg ctgacgagtt cggtggcccc
     6541 cgcaagaagg agcccttcag cctgaccgtc ctcaggcaca gcatcgaaat gaccctcgaa
     6601 gatcccgccg tggtcgactg ggacagcttc gttgaggtcg ggcgctcccg cggcgtccag
     6661 atcgaacagc gcgggaagaa ggggcgcggc atctcctacg gcatgctgcg cgaggagcct
     6721 gatggaacac gcgccgaacc ggcagcaagc gatcggcgcc gctgcacaac cctcggcacg
     6781 gcgtacgaga tgggcgcggt cgaggctgca tttgtgcgca acggcatcgc ttcgattgcg
     6841 acagcgcgag cgggccagac aaagaacgct cagacggcaa agcagcgcat gctcatggcg
     6901 ttggcagagg catccgcaga gggcgacgac ctcgcacaac ggatgatcgc cgagcacagg
     6961 gcccagcgtc cgacggtgat cgacgagcag cccgaatcga tggcagcgct agaaaccgat
     7021 ccgcaagacg cgatctcgcc cagctgtaca gccgtgacag tcctcgatga atcacgtcaa
     7081 gcatcccgta acgaagacga ggtcaactcg ctcacagacg gactcactgc ggccgccgaa
     7141 tcgcagccgt caccaatcaa gacggtgcgt gaagaggcag gcggagcggc acgaaaggtc
     7201 cgcagccttc atacccggtt cccggaactg tttgacgcgg agcgaccagc agggcaggac
     7261 acccaccact acggcctcga gtgaacgagc tcctgctgcg gcgccaggat gatctgtccg
     7321 taaagtgacc actagatggt cactttacgg acagttgccg gccactcgcc gtccgcacat
     7381 tccgctcgag ttggagtggg tagaaagtga ccgtgaactg gccattatct ggtccctttt
     7441 ggggccgcag ctggacacag ttccgaagcg ggcgtgacga tgcgggcgca gcctgaccgt
     7501 aaagtggcca accaatggtc actttacggt cactcagtct tcgtcgattt tcacgcgcac
     7561 gcagtcacac agtcggtcta cgccgttcgc gggacatgtc tgtgcggccc cgagtgcgag
     7621 tagtgcgtac ttggaatggc tcacggcagc atccctccac agagcgcgaa gacgcgcttg
     7681 cgcgcggcgc gcgcggagag atacccttct gggacgatgg ccgcgtgagc ctcctctctc
     7741 tggtccgtgt taaggagctg agtacgcctc gctactggcg acggcggcag ttgtaccaag
     7801 atcgacggca acgtctcgct cgtttcacat cgacgttccg gacccgggct gatggtgcac
     7861 gcacaagcct gaacgccgga tggcaactcg caatcagaag ctttggccag ctccttcttg
     7921 cctgcttcgt tgtggcagcc ctagaactga tcgctgcttg gctacaccag gtaccggcgt
     7981 tcgaagagag cccgctgttc agcccgccag catctagcct tgcggacgtt gtggcagcac
     8041 ttttcggggc agccgttgcc gttggcgcga ctctactcgg tctctactat gcaacggttg
     8101 gtgtgattgc ttcaaccata tacaaatccg tccctggtga cgtgcgggac ttgttcttgc
     8161 aggagcggaa caacgaaacg tatctcaggg tggtcatcct cacgatcgcc agcggcattg
     8221 ctgcgctcat tctgagcgtt tttggccgcc ccgtttcggg tctcacatta cttgctgtgg
     8281 cgggcctgac agctctgacg tgcatcgggc acgtggtcgt cacccgacgt ctcctggaat
     8341 acttcgatcc atccaaactc gcgttgccgc tgatgcgtca actcgcgaca gcaatccaga
     8401 cagcaacagc agaaaaatct cgggacattt ccttcaggca aaccgaggca catgcctcgg
     8461 ctttacgcgc tgccacttcg tatcgtcatc ttgctcagct gcttgaggaa tccgagctac
     8521 ggaacgcgac tgcgccagtc gttttgagca ggcaactgat ctacattctg aaactctatt
     8581 cgtcccggaa acacgccatt ccgaccagca gcaattggtg gagccgggtg ccccgccatc
     8641 agaattggct cacgccgagc cagaacagcc tggacctcgc gcttaacacc tccgtcggtc
     8701 ttccgcccga gttgcagccc gactacctct ggttcgagaa cgccatcggt cgggtacttg
     8761 ggaggagcct gaaggtcgcg ttccaagctc agggtggcgc ggatgcgttg gcagtttccg
     8821 aagaggtggc cgatcttgta gggcacctga ctgcgcggct ccaaattgac gaggccgtca
     8881 cgatcgcgac tatgtgggag cgtgtcgtga tggcggtggt tgcaaccccg caagtcgctg
     8941 ccgccgacgc tgacgataat gaagtacgtc tcaatcagat ggcagctgcc gagagcgttg
     9001 tgcgccccgt caccgtgatg ttgctcgggc tttcccacgc cgcatcgtca cttatccagc
     9061 gggacctacc caccgagttc gaggcagcgc tcagcaagaa tgacgtgctg tctgtcgagg
     9121 cgctacccac ggagactcgg cagatgctcg agagttttgc ggcagcaatc cgccgcgaag
     9181 tggctagcga gggtcgacgc gttacgccgg catggtgggt caatcatcta gccgcgagaa
     9241 gccttgctga catgcttctt cagacggagt cgggcattat cgcgctcatc aaggatcgcg
     9301 cgctcgagca cgtgtccgag ttcgatgagg cgggaaggcc agatctcgcg gtcgttgccg
     9361 gcatggcggc ccttgaactg ctcaataagc tcgaccacca tgagcagatc gtgcggcggg
     9421 cggaggagaa actcggttcc ttccgcaact cgaacattgg aattgagcgg tggccagcac
     9481 ggacggaaag tcttgacgcg ggcagcgaac atcgcgccct tctcgggcga ctagcctcgc
     9541 tcctgcctaa gctgcgcaaa gccgagttcg atcctcgcga gcctgacctc tacggccagc
     9601 tttatcagtt cgtcgtcgat ggagcgttcc acgcgatcct cgacggtgac gctgatcggg
     9661 gtctcagcat gtacaccgct gctcttgccg agatggagtc agcacgcgca cgcatcatga
     9721 gcgacctcaa gcagcaagac gcccagatcc gtctcgtcta cgcgatggaa ccgctgatca
     9781 cagcgatgga cctcgctgga tacgcactcc tcatgcatga gcttgacggc ggcgacattt
     9841 ggccggaggt gaagggcaaa tgggatcagc ttcttgaact gagtccccaa ctgccgagct
     9901 tcctgttgat ggcagcaaag ttcgtcgagg acatttatgg attgactgtc ggtggggtcg
     9961 agcggagtcg ccggtcgatc gccctcggcc agatcttcaa ggagcggaac ctcagccatg
    10021 agtgggagac gctcttggag gaagctaacg agcccctgca tccaagcccg atcgtttccg
    10081 cgattgcgcc gcggggcctg gggattcaag acgacctcta cgcactcttc atcgctgagt
    10141 accttgcgac attcctggag aatggcgagg atctggggaa caaggcaagg ctgatcgcgg
    10201 aagagataga gagataccgc gctgagcagc ctgacgacga caccgaggac ggacagggat
    10261 gatgcacgat gacgcgttcg ccggtctcgt ggctgactgg tccgagcaga ttccctacga
    10321 ggcgcgtcca gaaaccccgg cgccgcgccg gcgggaaatc cctgacccgg ccggcaagct
    10381 acatatgttc ctgcaggatc tgcgcacacg agcacttccg tatggatttg gcgcgaacga
    10441 tggattcggg ttgcgcctcg cccccgaaga cgcagatgtg gcgcgcctgg ttaccaacgc
    10501 gctgcccggt cgtgcatacc cacacagcca gctcgagcgc gcgttccgcg attacgttga
    10561 aactgccctc tggtacctga cgttcggaga ggtcttcctc aagcttgtgt attaccggcc
    10621 cgctgatcga cctgattcca gacccgtcgg attcagaatc gagttcctgg atccggagcg
    10681 ggtccaaagg cgtttcggtc gataccacta ctggacgctc aagctccatc cgggtcatga
    10741 cgtcgccgag tggaagagaa cacccctgcc gacagaggga ctggtcgtgg tgtccttgcc
    10801 gcgacgactc cggcggatga tggacagagc tgtcaagatc gttgatgcga ccaaccagga
    10861 tctcaaggtc atgtctgaat tcacgatggg ccgtttcgcg ggtgagcccg cgtttgacat
    10921 cgggcgctac aaccgtcaga ttgctgatgt cgtcctgcgt gagactcgcg cggtgggctg
    10981 gggcggtcga ggcctcctga ccgaaggcat gctggaccct ttcaaggctt ggcgaaatgt
    11041 tcagttcgcg cggttcgatg cgaagctccg cgatgtcgca gtcgacggtc tccaggacgc
    11101 gatcaatcga gcaggcaccc tcatggggtt tgatgccacc ctgcaactct caggagtcct
    11161 gggtgaggct gatctcgacc aactcgaaca tgatctgagt gctggcgttc gaccaatgag
    11221 tgaactcttc gccccgcagc cctcaggagg gtagcccaag tcaccgccca ccctaatcga
    11281 aaagagtggt tggtgccgga gtacgagcaa ccgccaccag ttgcggcggg tttggcggta
    11341 gctcgacgtc gtcgaatgtc tgagcgcgcc cacagcgcgc ggcacttccg ccggcgcagc
    11401 gcacctctgg gatattggca gaagcagcgt ttcggtgctt ctgcacgtac taggcgctcc
    11461 ctgcatcggc gaggtgactt gtgcctggcg aaatcgctgc agcacgtagc atcctgttca
    11521 accgctccag gaatcatcca ctagcgcctg agccgcgatc gacaccgctg tcagtcgccc
    11581 ctctgcaatc gctgccacaa ccgtgtcgtc atggagccat ggggtcgagc ccacgaacca
    11641 tgatcgggca acgtcttcgc cttctgcagc ggccactcgc tgccaaactc gatgcgcaca
    11701 tacgaggcgc tcgcggaggc cggcagctgg gaagggggtg gcgccatcag cccatcgctg
    11761 cacaacgcga caatctttct cccccacgaa cgccgcaacc agcgttggcc ccagtccgtc
    11821 gatcaggtcg cggaggagaa cgccgatggc agccgcctca ccgccagcat gcgcctcgct
    11881 ggcccagccc tccagcctgc gcagatcagc ttcagtcagt cccaccatcg ggtctggccg
    11941 cacgaggaac tgggcgggtg tccgcccgag ccgccaccga ctacctgggg tgcgcgtcga
    12001 gacatccagc tcatcgtccg cccatgcgaa gcgtgcgggc cgcagttgct cgatcagcag
    12061 ttccgccgcc cgcagcttcc accaatgagg ggactctgga acgaacgccc cccatgcgtc
    12121 gaagcgtgga tcgatgtgtg atctgccgtc ggtggtctga atagcgggat agccggcacg
    12181 gtgcagcaca atcccgtcga gccccagtac gtgctccaac tcgtcgagca accaaggttc
    12241 ctccagccaa gtggtgagcc atgcgccctc gacatctggg cgtagcaaca gcaaacgcag
    12301 ccggtccatg acagccggac gccatcgaat cggccacctc cccacgtgca ccgtcatgaa
    12361 gtacgggtcg gccttccgcc cgaaggagtt gagtacgccg tcaacgtcca acgcgatgac
    12421 cgtcggacca ggactttcag cgtcggagcg atctctcatg tgcagttgat atcagcgtca
    12481 cggccttgaa tctgacaaaa ctctgcgagt tacaaatgag cgaaagcgcg ctttcttgct
    12541 cataaatggg cagatccaac aggggacgcc tcacacaaat tctcctgcgg agcaagaaca
    12601 atcccggtcg tgcacctacg gtatatcggg tggatggtca ggacttcgat cgaacggtca
    12661 atgcacaggc ttcggcgacc tcagtccgag cgctcgtcga cgtgatcagt aggttgacgg
    12721 acgacccgga atggctgatc gctgcactga ccgagattat gagagccatg cgggcaccag
    12781 tcccgccgac ggaagcggaa gtcgagttcc tgctcagctc ggctgcgttc actccgacgc
    12841 agctctccag aatccgggag gaagttgcgc gaggctctct gacgctcgca ggtgcggaga
    12901 gtttcctcgc tgcgcttcat gccacctggt ctctcgagca ggtagcccgc tatgtggaca
    12961 agaccacgga cgacgtgttg acagccgtca aggtaggaaa gctgtacgcc gtcgcgatcg
    13021 ccgagcggct gcggttcccg gtctttcagt tcaacatcgg tcgtcccgaa cctctcatcc
    13081 ctcatctccc cgagctaatc gaggcagccg gcacgcagtg gagctggatc agcgtcgtcg
    13141 cgttcatgga aacacgtcaa gcgagcctgg tcgccgttgc cgcgcagact ccacgtgcct
    13201 ggttgctcga cggtggcagt ttcgacgaga tcaagcagat catcgactcc gcacactggc
    13261 gctgaccgtc ccatgcgtca gaaaggatcc gtgaatgtcc acgaacacat ggcgacagga
    13321 gtggcccgcg caccgggtgc gactcatcga catcaccgcc cggcacttcg tcgacgggca
    13381 agctcgcgcc agtcgcggcg agctgcccgc ttggaccgag ctcacgccgg aggcccagag
    13441 caacttctgt gacaacgtcg ccgcggtact cgccgctcaa gccctagcgt tcgccgagat
    13501 cgaagcggag ctcagcgcca agcaggctcg cgtatcgatg aatgaggcga caacgcgagg
    13561 cgcagatgct gccgcctcgg agacggaatc atgacacccc cggggcgccc gagtaaggga
    13621 ccccgtgatg ccatccttat ccgcccctcc cccgagctcg gcgctctgct tcggcgcgag
    13681 ggacgtcgac accgcatgac agctggcgag cacgtggtgg ccctcctcgc tcagcatttc
    13741 gagatgccga tcttcattcc tgagccttcg ctccggaatc ccgaccggta cgccacgagc
    13801 cctcatgtca cgcgcggccg agactcgatc tcggtgcggc cacccgtcga gttcggccgt
    13861 ctcattaagg agcgcgcacg agaatctggc ctgtactacg gcacgtacgt agtcgccgcg
    13921 ctcgaggaaa ttcttcaccg tcaggacaag tcgtgagcgc tgaacgagat acgaaacacg
    13981 gacatcgagg gatggagacg ttggagagcg cggggaccct cgacctcccc gacaagcggg
    14041 tgtgggtcat cggagaccta cacggcaaca caggttggat tcaaacactg tttccagcga
    14101 tgcgtcgatt cgacccgacg atgcgcacag ttcttcagct cggcgactac gggttcgatc
    14161 acggtggtcg cggcgccgac gccgtggact tctgggcgaa gaacgccggg atagagcgcg
    14221 tgttggtgac gctcggcaac catgaagcgt gggacaagat cgctccggcg caggacgagg
    14281 cgcccgggca ggcgatccgg gtgagcgaag tcgtgtggtt cctcccgcga ccattcaggt
    14341 ttaccgtcgc aggccgcgag gtgctttctc ttggaggtgc ctccagcgtc gataaggcgt
    14401 tccggaccgt tggtaaggac tggttcgagg acgaactcat caccgatgct atggagcatg
    14461 cggcgatctc tacggggcgc ggcgacgttc tattgacgca tgaatcgcca atacgttcag
    14521 tcccggaggt gcagtcaatt ctgaccgcca atccgttcga gtactccctc gaggccctac
    14581 atatatccgc gtcgcaacgg gcgcgggtcc agcgagtaag cgacgccacc cagcccgaac
    14641 tacatctgca cggccacatg cacgtctacg gcgacgttga cctcgccgac ggccggagga
    14701 tcgttagcct cgatcgagac accttcgcaa gaaacgctgg cactctggac ctctcgacac
    14761 tcgagttcac cccactgcca cacgccttca tcaaacagcg ttgatgcaag cggtcgctgg
    14821 ccgaaattca agcgtcggaa gcgtctccag gtcctccacc agctcctgct gctcgcgcga
    14881 atgatccagg cacagtcgga agcgatctct ctcgcagtcg catatggagc gacgacgcac
    14941 ccggagttcg cgacgctcgg ccgggtcgcg gcaccggata gggcagattt tcgcctacat
    15001 ggcaccaatg tccgccgcca cgaccgttgg cttatgaccg tcgagcacca ctggtcggct
    15061 cgtgatggag ttgatcgtcc agccattgac ggtcggggcg cgtagcgcgg ccacgatctc
    15121 atcgtgcgtc ccgtcgacgt cgagggcacg ggtttcgacg gggcggatct ggccggtggg
    15181 ctccagggct actgttatta ctaccatcgc ggacgcgcag gtggcccggg ggatgttgac
    15241 ctgcgcgtcc actcgggtac cgatgtcggg gcccccgccc cggtcgcatg cccgtacgtc
    15301 agaccgagaa gggaaaaggt ccgacgcggt ttgattctag agccgatcgc gtggctctct
    15361 ggcccttatg ctgcaggggc gatcacggac gggtcgctgg catccaccgt gcgggagttg
    15421 ttcaccctcc ggtcaatgac gtactgggtg atggtcccgg ccacgtcggc ggatgccgca
    15481 tcgaggaggg acagcatccc gtcgcggtcg ccgcggtcga gcttgaccgg ttcgagccat
    15541 gcggtcatgg catcttcggt gaggaacgcg ggcatgcggt cgtgcacctc gccgccggcg
    15601 tcccgcgctt cgcgggtgat gacgaccatg cagcgggacc gctcgccgtc cgggagttcc
    15661 atcgaccagg tgagcccggc ggcggcgagc agaccgtcac cgtggaggaa gtgtggggtc
    15721 ttgtcaccct tctcaccggt ccattcgaag tagccgcgca tcgggaccac gcaccgggcg
    15781 gcggtgaacg ccggcagcca gaacccggtg tccagcttct cgatgcgggc gttgatgatc
    15841 ggggcgcctt tggggcggtt cgctggcttc tgccagtccc accgcacgag ttcgagggtg
    15901 cggccctcgc cgcggtcgcg gacgatcacc gcatcgtctg tcggggcgat cgagtacgac
    15961 ccgtggaagt ccttccacca gtcctcgggc ttcccgccgt cggcgacgaa ctggtggatg
    16021 aggtcatcgg tcttggcgtc catcgcgaat cgtccgcaca tgaccgcgac cctacgcctc
    16081 agagtcgacg ttcgcctcct cttcccgtgc gatcgacgcg aggatcactc gcatttccca
    16141 tgccggccca aggagccgac cacggactgg cagatccgat gcgcccccat cgctagacgg
    16201 atccccttct gctttgcgct tagacaaagc cgtaaccttt caacagcccc ccagcggact
    16261 gccgctccta acggagctgc catgataaag agacgcagaa tccgggggtt cattacgcgg
    16321 agtcgtcgac ctctactgcg ttgccccaca cccagcaccg gaacgtctgt tcttctgccc
    16381 ggaactcgat gccagccgcc gtcggcgttg aacgcacaag cttggcatct acgcgcacgg
    16441 cctcgccacc gaagcgcacc catgcccaca cacggcgctg cagggcatgc gggtagatcg
    16501 tcgtcgggtt ctcccgtagc gccagctcgc gatccgtcag agactgcaga gggccccgct
    16561 tggatgcctc aatgaccgcc cgcgcgagcg cgcggcggtc gtacgcggct gccagccgcg
    16621 gcccaggact ccccatcgcc ggcaccccct tcctccggcc accatcaggg tagcaatctt
    16681 cgaacactcg ttctatggtg ttaacccatg acggccacca acatcgaaca cactgtaacc
    16741 atatggatgg tcaacgagat ccccgcacgg atggtctacg cgggccgacg gtggcgcgtt
    16801 accgacaccc cgacacgact ccgccactcg atctggaccg tgcccctcga tgagccacac
    16861 cacggcctgt acggatggcg attccaaggc accgacgacg acggcctatc gctcgtgttc
    16921 gacgtctacc gctgcgagga cggatggcac gtacaccgtg cctacgactg aactcgcaac
    16981 aatgttgcag agcgttctcg ctaccgtcga gacatggcga actccgaaga cgtctggtgg
    17041 cggctccccg acggcgtccc aattatcctt acgagcgtgc accgcggtcg cgcctactgg
    17101 gaggcgttcg atgggaccac ggggagcgtg acggaacccg agttcataag cacccaccgg
    17161 cctatcgcgg tcgtctatcc gcccaagatc gaggggccgt cgaactcaga tgggggctcc
    17221 acgtcctgag cctgctggac gtgaccgcag tgcgggcact cccaccctcc cggcacgtcg
    17281 cgcatgagga tgccatcctc ggggcatcgt ggctgcgcac cccattcgag atcctcatcc
    17341 attccgacag cgtatgtgcg cgaccgggta gaaggtagag gtatgccacc gacacgagag
    17401 aatgcttgat ccagctcgcc gatgtcctgc tcgagaaggc atgcgagatg gcttccgatg
    17461 ccagcccggc aaggcgcgtc cgagagtgac ggcgccgccg ttgaggaacg tccataacga
    17521 ttgtgggcat ccgcatgctc cctgctggcg ggccctcaga gcacatctcg gccgccggtt
    17581 ctcaagctct cagtgctggg gcagaggatg ccgccatcaa cctcagcggc ggggcacttg
    17641 cccttgtatc tcttggcgtc gatggcccag accccgcagg tgcgatggtg ctgtggtaga
    17701 tgcggagaaa cggtttcctc ggtcccggta gcggcggacg cagacgccga caaacgacaa
    17761 gacgatgcag agcaagaacg tcacgagccc atcgaaatgt ccatgggctc ccacgccaat
    17821 gccctgactc gcacctcagc cgcggtgaac gctagatgac acggcgataa gtcccagatg
    17881 ccggtggcga gtgtatgggc gtcgatgctg tgctggttcg tgttggcgag gcggcgggcg
    17941 agttcgcgac ggacttcgtt catgccacgg tgggcggcgc tccattgtgc gtgggcggca
    18001 cggtaggcct cacgcgcctc tcctgtccat tgggattgga ggagtttcga ctcacgatcg
    18061 agttcgtcca gcacgagcgc gatgttcttg ctcgcgatgg tcatctccgc gaccaggcct
    18121 gcgaccttgt catgtggcac ggcgatccgc atgggtcagc gctcttcgga ctgggggacg
    18181 ctgaggtcga tctcgcggag ctggtaaggc cgaccgacgg catcggtgag gtagaacgtc
    18241 atgaagatca ccgctgcctc gtcggcggta aagatctcat tcgtgaacac ccgcaccaca
    18301 tgcttgtcgc tgatcggcac cgtggtcgtc tcgtcatcat ggggttcctg gcgcccgacg
    18361 gcgtagaggt gcgcggtccc gtcggaggtc attacccgcg cctcgatcgc catcttgtcc
    18421 gacccgcccg cagcctggat gaacgtctca cccctcttca tcggatcgcc gcctggctcc
    18481 ggaagacgcc agatcgagaa ggtcgtacgc ttctcgccgt ccaccagccc gagcacgtat
    18541 cgcagataat ctgcggtagt cgtgctcatc ggcggctgaa agtatcggat gaaggtgccg
    18601 ttgctgtcct gggcgtgagt cttctttgcg ttcatgggtt gtgtacgact atcacttcga
    18661 tgactttgat ctcttcacca ttaagattca cgacggcggg gttgtccgga ctcacttggg
    18721 aatcgaattg ccgctgcgtg ttcccggggc gctggtagtc cacggttgtg aagcgctgag
    18781 cttcggcacc cacgtcttcg ctgccgcctg cacttcctcg accccgtgcc agatctcgag
    18841 cgcatcgttc agcattgtgt acgccgacgc ccaaacctga tggcctgtca ggatgccaca
    18901 aacaccgccg gcatctgtgt agtggtaggc cgttccggtg tgcctgcgaa gagagcccca
    18961 tagctagcgg tctggtgaat catgtaacgg agagtagtgc aggctgggtg tgtcacccca
    19021 cccgactatc gcaaacacat ccccacgcct acacgtgtga accaactgtc cagtgcctcc
    19081 tccgtgtgac agtccgcgga gacccgaaga catccttgaa gtggtacggc gccgccgagg
    19141 ccccgcgcat caaaccggac gggtcgctgc tgactaggga gaaatccgcc cgcgcttggc
    19201 gcagactatg gacatgcagc ttccgttacg tgcgcacggc ccttcgctga cgtcagatca
    19261 ggcgagaatg ctcgacgatg agttcttcca ctcgaacccg ctcggccatt tctcgtcgaa
    19321 gatcgcgaat ctcctcgagg cagagcgcac atcgtcacat ccgaccgctg agtcgtattc
    19381 ggagttcttc gatgcgctcg gtttgaccga atcggagttc ttgatccact tcgatgaaca
    19441 agagcgacgc gtgcagacag ccgttgaagc gctcgcattg cgccatcaag ccgcggaggc
    19501 gctgacccgc ttcacctatg cagtggcggc ttcaacgccg aacggcagtg atgctcgatg
    19561 cgtgtggctt gcgatcgccg atagccccac gaagatgatc gacgtgctga acgcgaacgt
    19621 cgatgctctc aataccgaag gcaggttcct cgaactgttc ttcccgccgg gcgccgttat
    19681 cgatcacacg gcgatccgag ccgccgacac tgctatcgcc tggctgaacc atgcggcttt
    19741 tctgctgtct gacaacgaac tgtccgtaaa cgctgccaat aacaaggtca agcacggact
    19801 cgccgtatcc gctcggggtg atgttcgcat agagttcgtg actactcctc cagacgccga
    19861 cggcaacatc cccgcgagcg ccttcggaga agggaaatcc gttcctctct tcgaccgcgc
    19921 gatgttgact tatctgtgta gacctcacag cacccctcgc caaggtgtcg aggctgtgtc
    19981 ccttcgtgtt gacctgccca tcgtgctggc cgaagcgtgg atgatggcaa acgtttatgc
    20041 tgcccttttc gtctctgcag cgcggcggca cttcgggaac gacctgccag atagcatcgc
    20101 cgagtacccc aggctggttg tcgatcgact tcccgagcac gtcatcggtg gtcgtgtcct
    20161 cggcttccgt tcgcccatca ccactccgcc ggacggagcc acgccgcacc ggccaattgg
    20221 cctcttcttc caccgacggt tcattcctat caacgtcgac ttcgaacacc ggttcacggg
    20281 gcgcgtcatc aagggctaga cgcacctgat caacggcact tcccggccgt ttcgctctca
    20341 tccccggaaa agggggcttc gcgccgatag ttcggataac tctgaatatc ggcggttaga
    20401 tcaacacccc ttgaagtgag atggggctga gtctgagctt aacgcgttga gtgcacgaat
    20461 acgagtggat ctcgcgtttc tgcacagaaa cggcggtgat gcccgggccg tgagcccatc
    20521 gctgttggcc cggcccatgc gatggatcgt ggtgatgcca gcggcgtggt cccgctttcg
    20581 tctggcatcc ggagaacgag ggaggggacg cctcgccggg ggtgcggatg ctccggcaca
    20641 ccggccccgc gcggcatacg caatcctgga agtctcgccg cgatccatgc gactcatcgc
    20701 gacatctaca atgctgcgga ttccgccaca ccaccaatag gtttctactt gcgaccgctc
    20761 atgaagctca gaagggcacc ccagtgatcg actcctcccc tgccgcgcta cgtgtccgtc
    20821 ctcgagtctg gatcggactt tcgatctgga tcggatacac agcagtcgtg ttcctcgtcc
    20881 agatgatcgc gggtgtccct tataccgaac tcggccgtga cggcgcgacg ctgttctttg
    20941 gagccggcct gtcattgatc gtgggcgcga tgctgcttgc gctcacgacc agccttctcg
    21001 gttggtggcg gccggcactg ttcgagcgga aaccgagcgc gcgctggccc atcttcgtgc
    21061 ccgcgatcat ggcggtcgcc ctggtggtca atctcgcgag tacggactgg ggctcctacg
    21121 acggcgcgtt cttcgccgcg tctctcgtgc tgctgctggt cgggtttacc gaggagatga
    21181 ccgcacgggg tctgctgctc gtctcactgc gcagtcgact cggagaaggc tgggtgtggt
    21241 tcctcacgtc ggcactgttc gggctcatgc acctggtgaa cgctgcgagc gggcaggacc
    21301 ttcttctgac attgcagcag gtcggaatgg ccttcctcgg cggcacggtt ctctatatcc
    21361 tccgccgcac caccggcacg ttgatctggg ccatggtgct gctcggcctc tgggacttct
    21421 ctaccttcgc cgtcggccac gggactccca gcgccttcgc ggccctcgcc ggcaccatca
    21481 atctcatcgc aggcgttctc ggcctgatcg ttgttgcgtt cgtgatccgc ggcgctgggg
    21541 agaagatctc gaccaccaag atggctgccg tcgcggcgta gggcacacct tgccctgcga
    21601 cgaacttctg ccgggcctgc cttcggtggg gagcccgagg taatcgaagt cgtggtcgga
    21661 gttcaggatc acagcgtcgt tggtaatcgc gtatgcggcg aatgccgcct gcttgtcggg
    21721 gtgtcaaggg ttggggcgag gactgcccga cccgtcgcgc gggcagccgg cgttatcggg
    21781 tcggcaggtt agaagaactc acgtatgtgc gccgccgtcg attctgatct ccgtcccagt
    21841 tatgaaggcg ccgtcctccg agatcagcat ggcaatgacc cctgcgacgt cttcgggccg
    21901 ccccatcctg ccgtcgttca gcggggcgcc gagatgctcg agcaaggtcc agtctgtgtc
    21961 cgcggggagg ttctgtgggg ttgtgttcgt gatcccgctc gtgatccctc cgggcacgat
    22021 gttgaccgcg cgaagtccgt gcctggcgta ctcgagcgcg accgagtggg tgaacgacgc
    22081 gatggctccc ttggtcgcgg cgtatgcagc catgtagggg tgcgcgaaga acgccgatgt
    22141 ggacgcgaag ttgatgatga cgccactggt cgtcgcaagc agatgcggaa ggctttctcg
    22201 caccatcagg aacgtcccgg tcagattcac ttggaggagg cggttccatc ggtccagtgt
    22261 catctcgtgg gtgtggtctc cgtagaggat gcccgcggcg ttcacgatcg cgtcgagtcg
    22321 tccgaagtga gtgaccacgt ccgcgacggc agtgcggaca gactcttcgt tggcgatgtc
    22381 gacgcggtga acgaacaggc gagaccttgt gtcttcaggt gcgatctcca cgagcgcggc
    22441 gagatcctct tcgcgcaggt cgagcgcggc gacgtgggct ccctcctcga gcagccggag
    22501 ggcggtgccg cgtccgatgc cggaggcggc accggtgacg atgatgatct tgtctgtgag
    22561 ccgggcggtc atgtgtgctc cattcatgcg gggatcgata cggacggtgc ggaagatgtg
    22621 ctcggtgccg atgcggctga aatagtgcgg tcggtcactt cggcgagaac gacgacatca
    22681 ggggcgcctt cagcggtgac tgtgatctcg acggcgccgg cacggtcggg tcggatgacc
    22741 gcgaggacac gaccgtcgta ggtgccgtgc gtgttctcgt cgaatcgttc ggtggtgcgg
    22801 gggcggccgc tgccgaaagc ggcgagtgaa ccggcgccgt tcacggtcac agtgacgggg
    22861 cggtcggcgt cgttgacgag gttcccctca gggtcacgga gttcgatctc ggcgaagaag
    22921 agcgcgccgt ctcgaagttc cgtggtggtc ttgtcgagtc ggacgtcgag gagtgcgtcg
    22981 ccggccgctg atcgcagaac agaccgtccg acctcttcgc cgtcgcggag agcgattgct
    23041 gtgagggttc ctgcttcgaa cgggatctcg aagtcggcca ggaaggcccg gcgcgcgccc
    23101 acccgttcag ccgcgaccac gcggtcgttg agctcgagcc tgatctcgtc tgcgtcgctg
    23161 tagacctcca gcgtcatggg gaaaccgtcg tcgatgttcc agctccaact ggatattgcg
    23221 tccgtccagc tccactgtcc gagggtgaac gcgcgaccgt ggttcgatgg tcgccgcacg
    23281 gcgatgtaag gctcgctgcg gaggccgaag acggtctcgc ggtagtacga catcggccgc
    23341 cggcgcccgg tgatgtcgag gtcggccgac caggacgtca gccacgggaa ctcgccgtcg
    23401 aaggcatcgg gctcgccttc gtacttcacc cggccgacgc cgacttcgcc gaggtagtcc
    23461 catccggacc aggtgaagtc cccgatcacg tgcgggtgtt cggtgacgag tttccagttg
    23521 cgatcaatgc gcgacgggaa tgtctcggtc ccgacgacga tgcggttggg gtgttcgcgc
    23581 tccatctcgt agcgggcatc gccgtagttc aagccgacga tgtcgaggat cgcgaacgat
    23641 tctgctgtcg ctgcggtgac cagaggcgcc gtggcgaccc agtccatgaa gtcctcggtg
    23701 ctccccatga gcgcgttcac tccgccgtcg gcttcggact cctcggtctc gctcgaggac
    23761 gaggcgtttt cggtggcggc ctcgatcatg tcggagatct cgctcagcgc agagacgaaa
    23821 ccgttgatgc cgttggtcac ataacgggtg ctatccagtt ctcgaacctt ctcggcgagc
    23881 ttgcgccccc actggctggc gatcgcgttg cccgtctccg ggatctcgtt gccgatggag
    23941 tagatgatca cgctgggatg attgaaatcc gtggccacca tcgattccac gtcccgttcc
    24001 caccactccg ggaacgccag ggagtagtca aagggggact tcgattcggc ccacatgtcg
    24061 aatgtctcat ccatcacgag catgccgtgc cggtcgcacg cgtcgagcat ggcgacgctc
    24121 atggggttgt ggctgttgcg gatggcgttg aatcctgcgg ctttgagaag gagaacgcgt
    24181 cgctcttccg cgtctgcgta tgtggctgcg ccgaggatgc cgttgtcgtg atggatgcac
    24241 gcgccgcgca gcttcactgt ctcgccgttg atgcgcagac cgtgggtggg atcaagctgc
    24301 agcgtgcgga tgccgaagcg cgtgtgccgg gtgtctgcca cgtcggagat tccatcgaga
    24361 gtcgtgcgtg ctgtatagag cgcggggttg tcgacgctcc atctctcggg cgtgggcaca
    24421 tagatccgct gcctgacggt tccctccgtc tgaggaagcg tggtgacagg gctccgctcg
    24481 acgccgacca ctcggccgtg cgggtcgagg atctcggtgg tcaccgttgc cgttcgagtc
    24541 aaggtgccgg agttggtgac gcgggtagtc acgctcacca ccgctcgctc gttatcgacg
    24601 tcgggggttg tgatttgcac gccaccttgt tcaggaatgt ggacgcgttc gctgacccag
    24661 aggtgcactt ctcggtacag gcccgccccg gtgtaccagc gagaatccgc gtgggtgcga
    24721 gcgtccactc ggatcgtgtt ctcagcgccg tactccaagt agccgtcgag gcagacgaca
    24781 aatgcggcgt acccgttagg ccgctgggcg acgaaggcgc cgttgacgta gatcatcgcg
    24841 tcgcgataga cgccttcgaa ctcgagagcc acatgtttgt cttgccagtc gaggggtgcg
    24901 tcgagagtgg tccgatactc gaatgcgcct ccgggaaaga acgcgccggc cgcgccgctg
    24961 ggcgcgtccg cgtgccgttc gagggagatg attgcgtcgt gagggaggtg tacgggcaca
    25021 ccgggtgatg actgcccatg cgcgtcggcg aagatgctgg tcttgggccg gtaagtccag
    25081 ttggcgttga ggggtgaacg gctcattgtt gcctttcgtg cgtgggatcg tgtcacagac
    25141 agtcaggcag cggccgtggc agtccattcg gaacggatgg tgtccagcgt ctgcaggacc
    25201 gcgacgctgt cggacagagg atggtgcggg tgctgagtcg cgccttcgga cagggcttcg
    25261 tggaccgcgc gaagcatcgg aacatagccg aagccctgtt gggggaacac gcgatcggtg
    25321 ggttctccga acgcctcgcc tagatcgcct gcgtgcaacc tgtatcgatc ggtggcccag
    25381 aacggagggg cgatcgtcgc ccagccgttc gtcccgctta ctgcagcggt gggctccaaa
    25441 taggtcacca tcgatgctgc gagttgcgcg aatcgccctc cggcgtatcc gagggtggcg
    25501 tgaagtgtga gatcgacacc atccgctctg accgtcgctt cgcttgtcac gttcagcggt
    25561 tccccgagta cgtctcgcgc gagcgtcacg gcgtagatcg cctgatcgag gagagtgctg
    25621 ctgcgtagct cgctcgtcca gcgctctgac tccgggtggc cgaaaggtag cccgaacgcg
    25681 gcacgcacgc cgctgatatc accgagcgcc ccggcggcga tatcctctcg cagagcgatg
    25741 taggcgggcg cgaatctcat ccacatggcc tccatggcaa agcgatcatt cgcttctgca
    25801 gctcttgcga tggcccgagc ttcgtcggcg ttgacaccga tcggcttctc gacgaggacg
    25861 tgcttgcctg cctctaggga cgcgatcgca aggcgagcgt gcgtcgcatg cggcgtcgcg
    25921 atgtagacga tgtcgatgtc cggcgcggca agcacatcct cgatcgatgt gaatgctcgt
    25981 tcgaatcgat gcgtcgtggc gaacgatgcc gccttcttcg cactccgcga ccacacgccg
    26041 acccgccgca ccccatcgac gatgctcaga tcggcggcga ccgagtgcga tacggtgccc
    26101 gtcccgagga tcgcccatcc gaacgcggcg ggtcggttga tcatggtgtc accgtgaggg
    26161 tttctgtgaa cggtgcggtg gagtcgccgc cgatcgcgac gtcgaagatc gtcgcgtcct
    26221 ggatggtgga cgaggtgacc gcactccagt actgaagctg ctcaggcccg agcttgaact
    26281 caagacgacg agcttctccg gcggcgagcg agatgcgacg gaagcccttc agctctcgaa
    26341 ccggacgcgt tgaggtcccg tggcgctgat ggatgtagag ctggacgacg tcatccgcgt
    26401 cgcgatcgcc ggtgttcctg accgtgacgc tgactgtcgt cgcttcgcct cgcgcgatct
    26461 cggatcgctc gacgctgatc tcgctgtact cgaaggtgga gtaggacagt ccgtacccga
    26521 aggagtagag gggcgcgttc gattcctcgt agtaccgtgc gtccttctct tcggggagga
    26581 acgtgcgatt gtgtgcgtag atcaggggaa cctgcccgac atgccgcggc caggtgaacg
    26641 gcaaccgccc agcggggctc gtcccgccca gaagcaccga tgcaaccgcc tcgccggccc
    26701 gggttcccgg gtaccacgcc tgcaggatcg ccggcacgtt ctcgtcagcc caacggaggt
    26761 cgagcggacg cccggagacg atgaccagga tgactttggt gccagtggct tgaatgcgct
    26821 ggagctgctc gagctgtcga ccaggcaggt ccagcgtgct tgtcgaggac ttctcgccga
    26881 tctggttctg tcgctgtccg accacaacga tcgtgacgtc ggagtcctcc gccaaggcga
    26941 ccgcacgttc gatcgccgca tcgtcatcgt agccctctgg tgtgcgctcg actgtggcgt
    27001 ccatcacgtc gaacatcgac gggtagatac gttcgggaat gccagccccg ggctcgaagc
    27061 tcaccccacc ggaaccgagc gcggtgcgaa gtccgtcgac gatcgtgacc gtctcggagg
    27121 tgtcgtggtc gaacacccac gggccgagca gatcacgcct gctgtccgcg agctgaccga
    27181 tcaccgcgat gcgtccgacc gcggaggcat cgaggggcaa caccgcatcc tcgttcttga
    27241 gcagaaccat ggatgcttcg gcaaccgacc gagcgagtat gcggttctcg ggatcggtga
    27301 tcaccgctgc ggtgctcgcc tcatccgcat acgggttctc gaacaggccg aggcggaact
    27361 tcgcggtcag cactcggcgt acagcggagt ccaggacgct ctccgtcacc attccctcgg
    27421 cgagggcctg cgggaggctg tcgaaggcag gctcgtacat gcacatctcc atgtctagcc
    27481 cggcgtcgag agctcgggct gcggcgtcgg cgtggtcttg cgcgaagtgc tgcaccgaga
    27541 gtgagcgcac agcattcgcg tccgagacga cgaatccgtc gaagccgagc tgttcgcgca
    27601 ggatgtcggt gagcagggtc cggctcgccg ctgcaggtac tccgttgagg tccatgtacg
    27661 cgctcatgac gtttcccgcg ccagcatcga tcgcggcacg gaacggaggg atgtagacgt
    27721 tccacagctc gtggtccgac acctcggcat cgtcatagtc gcggccgcct cgggaggcgc
    27781 cgtacccgac gaagtgctta ggtccagcga gcaggcgatc cgttcccaga tcaccctgga
    27841 agccgcgcac ctgcgcggcg gacatcgccg aggccagcac cgggtcctcg cccgctccct
    27901 cgatgatgcg accccaccga ggatcccgcg cgatgtcgat catcggagcg aaggtccagt
    27961 gcacacccgc tgctcgtgcc tcccgggcgg ccgcggcctg cgcggcttcg accgtctgct
    28021 ccgaccaggt cgcggcgagg gcgatcgggg cagggaagat cgttcgccac ccgtggacga
    28081 cgtcgaagcc aaacagaagg gggatgccga gacgtgactc ctcgacggcg tacttctgca
    28141 gcctgttact cagcgcgggg tcgcgcagga acagcagaga gccggcgcgt ccggagcgga
    28201 tcgcggcctc gatgagcgcg gggagctcga cgtggatgcg atgctccggc gggagggaat
    28261 ctatatcgaa gtcctcgggg atgggctggc cgtcacccgt gtagaaatac tgcgtgagct
    28321 gccccgcctt ctcttcgacg gtgagccgcg acagaaggtc ttcgacgcgt tcgtggatgg
    28381 gcaggttcgg gtcccggtag ggcaggacag tcatcggttc ctccgtggaa tcgtgagtca
    28441 ggggggtggt gatggatggc gccgttgaag atttagcgcg ctggtccctc gagcgcgctc
    28501 tgcagttcgt cgaccagctc gggggacgca ccgggcacgt agtcgagcat cgtgcggatg
    28561 gacatccctg cagcctcggg cgccttgccg tcgagcggaa cgcccgggta atggcgggca
    28621 aaggcttcgg tgaagcggtg ccatcccgcg gggtcgtcag acacctcccg aagggtcgag
    28681 tccaggctca gcggtgcgcg gtcggcgacg ggggcgacgt cgtactgcca cgagtgctct
    28741 cctccgcgga tctcgatgac cgcagaatcg gggtgcaacg gcagaacgac ttccgcggtt
    28801 gtgcactcgg ggatgctgac atccagacag acctgactgc cctccagccg ccagccgata
    28861 cgcacctgtc catggacggt gtcgtgcgtc aggtctgcgt gggtgaggtt gccgcccggc
    28921 tgtggtgcga tgcgcatgcg gcggtagccg ggggtggtcg cctcaaggcc gccaatcgtg
    28981 cggtgcatcc agtcgacgac ggcgccgagg gcgtaatggt tgagtgatgt catcccggac
    29041 gggttgatcg ttccatccgg gcggacggag tcccacctct cccagatcgt tgtcgctccc
    29101 atggtcacgg ggtagaggaa ggacggggcc ttcgtctcga ggagaaggag atacgcctcg
    29161 tcaaggtgcc ccgtggatga cagagcatcc gtgaccagcg gcgtgccggc gaaaccggtg
    29221 gagatgcggt agcccgcccg ggcgacgact gcggccagcc ggtctcccgc atgctgccgc
    29281 tgtgcctcat cgagaatccc gaacgagatc gcgagtgcat aggcagtggc ggtctcgttg
    29341 accaggcgtc cgttcgggct cacgtattcg cgccggaaag cgtcacgaac tctctctgcg
    29401 agcgcttcga actcggcggc gtcagcccgg tgtccgagga cgcgtgcggt gtccgccatc
    29461 tcacgagtag tgcggcacag gtatgcgccg gcgacgaggt ggcggtccgt cttgccgccg
    29521 gcggggttgt cttcgggagc gtcggggtcc agccagtctc cgtactggaa tccggtgctc
    29581 cacagcccgt catcgtcgag gaggtcggcg acctggcgga tgaaggtggt catcgacgcg
    29641 tacgcgtcct cgaggatctt ctcgtctccg tactcgcgat acagtgccca cggaaggctc
    29701 acggctacgt cgctccagag cgctgtcgga gaggaggggg tcgacaacac gtccggcacg
    29761 acccagggca cgaagccctt ctcctgctgt tcgacagcga ggtctttcag ccaggatccg
    29821 aggacgccgc ggacgtcgta gaggaatgca gccgtcgggg cgaatgcgtt gatatctcca
    29881 gtccagccga ggcgttcgtc ccgctgcggg cagtccgtgg ggacgccgac gaagttgtcg
    29941 cgcatggacc acacggcgtt ggagtgcaac tgattgagct gggggttcga ggtcgtcagc
    30001 cacccggtgc gggccatgtc ggagtgaacg acgatcgcgc ggatcgcatc agctgacaac
    30061 tcgccgggcc agccgtccac ctcgacgtac cggaagccgt ggaacgtgaa acgcggctcc
    30121 catgtctcgg ccgggccgcc gcggagaatg tactgatcgg tggccgcggc ggtgcgcagg
    30181 gtctcgacct cgatctctcc gtcgaccatg acttctgcgt ggcggagcgt gatgatcgtt
    30241 ccggagtcac cctggaccgt gaagcggacc cagccggaga tgttctgccc aaagtccaag
    30301 acgagcttcc cggagggcgt gcgcatgagg tcggcgggcg cgagctcttc gatgcggcga
    30361 atcggcggcg cgatcggtgt ggtgagagct tcgagcggcc actcgaaggt ctcgaccgga
    30421 acccacccgc tgtcgtcgaa gcccggacgg tcccaccctt cgggctcatc gcgagcgtcg
    30481 tacgtctcac cgtcgtagat gctgttggca ccaactccgc cacgtgacgc ggtggtcgat
    30541 gtgtcgctgg cgatggtgtc gcggctgccg tctgcatact cgagatcgag ttcgaaccac
    30601 gcggccgggc gatcggagta gtgatgacgg cggtcttccc agcccagccg gccgacggcc
    30661 catccctcgc cgagtacggc ggcgatgacg ttcgctcctg cagtgatccg gtcggtgacg
    30721 tcgtgagtgc tcacgatgag gcgatgccgg tacgaggtcc atccgggtgc gagtacttcg
    30781 tcgccgacga cggatccgtt caggtgcggt tcgatgagcc ccacggcggt ggatcgaagg
    30841 gttgcgcgac gaggaacgct cgacgcgacg aattctttcc ggagatatga ggcgtgctcc
    30901 ccaacggcgc cctgggcgaa gggcgggctg acgaaacgtg cggatcggga ttcggacatc
    30961 ggatcggcgc tccttgagag agagcttcga cagtcgaact gtcgagactc cattgtctaa
    31021 gacgtaacta gcgcgagcta gctacggatc agaatagcat gagtgctagc gcgcacaagt
    31081 caatccctaa agccacgggc cgactgtggt ggacttgatg tggagcacgc cgagcaacga
    31141 aggagtgaca agtggcatcg cagacatcag gccgtcgggt tacggcggct gacatcgctc
    31201 gatctcttgg ggtgtcccga gcgactgtgg ggtttgtgct gaatgagact cccggtcaga
    31261 cgatctccgc cgcgacgacc gagcgcgttc tggcggaggc caggcgctta gggtaccggc
    31321 cgcacatcgc agcgcgggcg cttgccagtg ggcgaagcca catcgtcctg cttgtattgc
    31381 cggactggcc gatcgagcac agcctgcgcg agaacatcga tgaggccacg cacgcgcttg
    31441 atgagcacgg gtacgccctg atcacgtata ccccccacga aggatcgcgg gcgcgcccgt
    31501 tgtgggagac gctgcagccc gacgttgtca tgggtctcct gcccttccct gacgcactca
    31561 tcgagtccat gcgcgacgcg ggtgtcgaga ggatccttcc ggatcccgat gaggtcacgg
    31621 atgtccgcta caacgagggc gggccgcgac tgcaagtcga gcatcttcac gacctgggcc
    31681 atcgaaaact cggattcgcc cgtccgagcg atccgcgtct ggtcgagctc gcagacgcgc
    31741 gcgaggaggc cgcaaccgct gcagcggttg cactcggcct cgacgttccc gccaccttcg
    31801 agctcgctcc cgcagcgtcc gaggctgatc cgcccgtcac ggaatgggtc gaatccggga
    31861 tcacgggcgt ggtggcgttc aatgacgatg tcgcggcggc catcatcgga gcggccgccc
    31921 gcgcgggtat tgcggtgcct gagaaactgt ccgtcatcgg tcacgacgac actcctgttt
    31981 ctcggttgtt cgaaccgcgg atctcgagcg ttcgcatcga ctcaacgggc ttgggtcagt
    32041 acctcgccgc tctcgccatg cacgcgatgg accagacacc gacgccggat tgggagcaga
    32101 cctcggccgt cactctgatc gcgcgagcgt cgacttctgt gagcccgatc gcacagggct
    32161 agcacagcaa ctaagtgggg ccctcagcga acttcgccga gggccccgta tatctggtcg
    32221 gtttaccgta cctgcctcac cctcatcacg aggagtccgc caactacggt gatcgctccg
    32281 gcaacgaagt agagcagcgt gtagttcttg tctcccgtgg cgctcgcgcc gattgcaagg
    32341 acgagcgggg ccaggaaggg ggcgatggcc tgcgggatcg ccgtggcgaa accggcgata
    32401 cccatgaagc ggcccgcgtc cgtatcgcgc tcgggaagaa catccagaag gagggcctga
    32461 tcgactgcgg agaaagcccc gatacccacc gacgcgatga gcgacccgat gaagagcatg
    32521 gtgatgtcag aggccactgc ctccaccaca acgccccccg cgaagatcgc ggatgccagc
    32581 aggacgaaca ggcgacggcg gcggagcttg tccgagagga agccgccacc gatcgcgccg
    32641 atcgtggttg ctgcgatacc cacgagactc agggttgcca ggacgccggc gatctcctcg
    32701 acgctgatgt tcagtcgggc cgcgaagaag aaggcggtga acgtagtgtt cagcgtcaat
    32761 ccgaagtaga agcagaaccg gcccgcccac accaacgaga agtcaggata cttcgccggg
    32821 ttgtagaggt acttccggaa ggtcgttccg atggtgagga caccgtccac gatcatctgg
    32881 cggctgtcct gctcgcggga gaacagcagg aacagggtca cgaagacgac accgacgatg
    32941 ccagggatga ggaacagcag gagcgggtca ccgactaggc tgccggcgat gatgacaccc
    33001 gcgacgggag ccacctgggt ggcgaacccg acaaaacccg cgaccttgcc gcgctggtgc
    33061 tcgggcagcg tatcggccat cgaggtggtg aggttggcaa gggactgacc ccatccgatc
    33121 tgagcgagga tccagcccac ccctagcagg atgacgctcg gcgccaatcc catcacgaac
    33181 agggagagga cgccgacgag cattccgccg aggatccagg ggcgtcgacg accgaggcgc
    33241 gaacgtgtcc ggtcgctgag gattccgacg atcggggtga tgatcatcac gactacggag
    33301 cccgcgccag tgatgtatcc gaggtactcg tcgtggccag gggccagtgc actcacccgc
    33361 acggcgaggg agatcgcgat cggggtgatg aacgccacaa agattccgaa ctgcgcgagg
    33421 acgagcagcc agatgtacga gcggctcgcc cgagcggggc ggatcaggcc tgtcgttgtc
    33481 cggatgccct cctggatggc gcccggtgcg gcgggaagag gtgactgaag ctcagcccga
    33541 gggctgtccg cttcagaacc agaaacgttg ggcgtggtca taaggactcc attgtcgagg
    33601 tagtgccggc acgggcacat gttcgggact gcaggccgtc agctacggga tgcgaacggc
    33661 aacttgcgcg cgcaagcaga ccgactgtag caaccatcac ttgcgcgcgc aagtcccgga
    33721 ttcgtaaccg cgaggttcga ctgtcgaggg tcgatttcgg agtcgatcag ggtgggccac
    33781 ggtgaatcat ccgcaggtcg acgccagacc gtgtcgcgga tcattttgtg cttaccctcc
    33841 cacagggtcg agcgcatcct cccccagctt cagatgcctc gggcgggcac atttcaatca
    33901 cgcggcaggc ctcacctgcg caagtcgaga ctggcccctc gttatgtggt tgcgcccgga
    33961 cagctgatcc acgtagtaga ccaaaatcaa ggccgagccc ccccctcggc atcggcggcc
    34021 aagtctggca gcccgccttc acaacgcccc ggggaatgtt gtctagtggc gcatctcaac
    34081 cgtcgcgacg agcctcgtaa gacttacggc gcgtccgtgc gtcacgtcgt ggcaacgctc
    34141 ttcgcagcaa ccgctggcga cgccacggag ccatgttttc tcggcgcccg attcgttcga
    34201 atgtgcggtc gtgtttgcgt gcctcactcc acggtgggcc ggtctttctt cagccagcgg
    34261 atagggccgg agccttcgcg tccgagtacg tcgccgggat tctgcagggc gcaggagttc
    34321 aggcttaggc agccgcaccc gatgcatcca tcgaggtcgc tccgaaggtg ctccagctgt
    34381 cggatgcgcg cgttcaactc atcgcgccag cgctgcgaga ggcgggccca gtcccgcttg
    34441 gtcggtgtgc gaccctccgg tagggactct agcgcggagc ggatgtcctt caacggaatg
    34501 ccgacgcgct gggacacctt gatgaacgcg acccggcgca gggtgtcgcg accatagcgg
    34561 cgctggttgc ccgctgtgcg cgtggagacg atgaggccgt tgcgttcgta gaaatgcagt
    34621 gctgacgtcg cgatgccgct gcgacgtgac agctccccga tggtgagctg tcgactcttg
    34681 tcgccgtcga gttggggcac agagcctcct cgggtggaat tgacctcaac tttagttgat
    34741 gttttagcgt cgtcgtcatg actgcagata cgcgagaagc gggcacggcg aacgccgcag
    34801 ctccgggcat cttgagccgc acctacctct ggatcacgat gggcgcgtgc gcgctcgtgt
    34861 tcctcggagc cttcgaatcg ctggcggtga cgacggtcat gcccgccgtc agcgccgacc
    34921 tcgatggtga gcgcctctat gccctggcgt tcgccggtcc gctggcaacc ggcgtgatcg
    34981 ggatggtggc agccggcatc tgggctgacc gacgcggacc ggtcctgccc ctgtactcct
    35041 cggtcatcgt gttcgtcgcc ggcctgctcg tggccgggct ggcgccgagc atggaggtcc
    35101 tcgtggcagc gcgcttcgcg caggggctcg gaagtggcgc tctgacggtg gcgctgtacg
    35161 tcgtagtggg ccgggtgtac ccgcaggaac tccatcccgc gatcttcgcc ggattcgcgg
    35221 cagcgtgggt cgttccttcg ctgatcggac ccacggtcgc cggcgccgtg acggagatct
    35281 ggagctggca ctgggtgttc ctcggcgttg tcgtcctcgt cttcgctgcg ctgctgatgg
    35341 tcgtgccagc tcttcgcggc ctcactcacg gcggggacga cacagtgccg tgggcgttcg
    35401 gacgactcgg ctggtcggtg ctcgcggcgg ccgccgtcct cgcatcgagc ctgttggtcg
    35461 acgtaccggg gatcggttca ttgctcgccg gggctgctgt gatcgtcgcg ctggtcgccg
    35521 tccggcccct ggtcccgcgc ggaacgcttc gtgcgcgacg cggccttccg tccgtcatcc
    35581 tggtccgcgg actctcggcc gcggccttct tcggcgcgca ggtctatctg ccgtacctgc
    35641 tcaccgatag ctatgagttc acgccgacct tggcagggct gtcgctgacc ggtggggcgc
    35701 tggcctggtc gtgcgcggcc accctccagg ggcggatgac agcaacactg tcaagcatca
    35761 ccgcggtgcg gatcgggacc atcctcgtgt tgagcgggac tctgttggcc gcggctactg
    35821 ccgcattcca ctgggatgca gcgatcatca tggtgtcgtg ggtcgtcgcg ggaaccggaa
    35881 tggggctcat gaacccccga tccagtgcgc tgacattgtc tatgtccaca cccgaaaacc
    35941 agggattcaa cagcgccgcg atgactgtct cggattcctt cggaagcgcc ttggcgctca
    36001 cgatcacagg cgcggtgttc gcggtgaccg cggcggcgat ggaccccttc actgcggtac
    36061 tcgtcttgac cgtcgcgatc ggggtcgccg ctgtcgtggt ggcgccgcgc gtccgagcag
    36121 gggcaactgg cgtatcggct cccgagaagc gcggatcaag aacgccgcca cggtgagcgg
    36181 caggcgcgca tctgtcgagt cttgccggcg acgaggccgt ggcgacggac attcgcctgg
    36241 cggagacgaa gccgcgaacc aaagccatcg gccaacggta gagcgactac gagctctagg
    36301 cggtagcgtc aggcgctcga ctcgtggtga ggaccttgca cccgcccgag cgctgtgtgg
    36361 acggtggcga gttcgtcgga ggagagagtt ccgaagaggt tctccgcgac ggtgcgcgtg
    36421 tgacgctgcg ccatgacgag tgtggcgtgc ccgtgcttgg tcagttggaa aagctggctt
    36481 cgcctgtcgc gttccgatcg agtcttcgtc accaggccct gaccctccat gtcgatgaga
    36541 gtccgaaaca tccctgcttt gtcggtctgg agttcgtagg tgagatcgcg gtgagtgcgt
    36601 actccgtcac ggatgaggaa catggctgcg acatgtcgat agctaagccc gagcggcttc
    36661 agcgccttgt tgagctgcag cgttgcgatg gcgtacgccc gatgcagttg cagtgcgacg
    36721 tgcagggggg atacggcttc ggcgtccatg gaagctcccg cggttcaagg attgtttcaa
    36781 atgaaactat atagatatgc tggcggcatg agcacggatg acagacccgt tccggtgtgg
    36841 cctcggatgg gcctgcgggc gtcttcgtgt atagcggccg gcctcctcgc actgcagtcc
    36901 ctgaccgccg ggctctttct tgcgggagta gaggccgcgt tcgacgtcca tcgtgagacg
    36961 gccaccgctg ccgggatcgc gatcatggtg ggcatcgtgt tcggcgtgat gagcgtgcgc
    37021 ctgcgaggtg agtcatggaa accggtcatg tggagcgtcg gccttctcgc tctcatgtcg
    37081 ttgcaggctt tcgccgggtt ccgatcgctg accgcccttc acgttccact cggagtattg
    37141 accatcttcg ccggcgtcat ggtcaccgct gtgtcatggg gctcaccatt cgggcgcgcc
    37201 gagggccgca cgagcgcgga tggctcaggt ggccgcagat cgcgccgcaa caagtgaccc
    37261 gcgccgtcga ctgacggcgg tctcgctgtt tgcgagtctg cgatgcgcac cagctcgggg
    37321 cggggacttt atgcgggaga gccgcttagg gctctcccgc ataaggtctg tggtgccgcg
    37381 ggtcatctcc cgggcgtcgt tcgtggccgc agctcggtca cgacgatcgc tgcggcgcag
    37441 cccgtcacga gaatgatcgc catggagaac ccgaggcttt gattggtgag atgggcaaac
    37501 gagccgagga tggcgatgcc gatgctctgg ccgacttgcc gggcggtgtt gtagatgccg
    37561 gagccgaggc ccgcggccgc gtagccgaga gagcaactgg cggtctggtt cagggccgtg
    37621 gagtagatcc cggtgccgat tccgatcacg aactcggcta ggagcagcag ccaccacata
    37681 tgtccgcctg acaccaggag cagcgcgagt gtgccgatcc cggcaacggt cgcgcccgtc
    37741 agggtcatcg cgcgggcacc acggcgttgc agggccggat tgatgacgac gcccgcgagg
    37801 acgacgccga tcgcgaacga cgctagaagc agcccacccg tgaacgccgt gacgccccaa
    37861 gcagcttgga ggtagacggc gagaaagaac tggagcccat atgcgccgaa ttgaagggcg
    37921 aacccaccga gcactgcgcc gggaaatccg ggagcgcgga acacggacag tggcatcatc
    37981 ggcatcgtca cccggtgctc gatcgcgacg aaagcgcaga ggagaagggc accgagagag
    38041 atcaaacccc agctcacaaa cgggctggat gctgagccgg cttcgatgag cccggcgacg
    38101 agcgccacga gccctgctgt gccagtgagt tgcccaggca gatcgacatg caacctgcgc
    38161 gatgagtcgc cggggccgtc ggtgtgcccg gcgaggacag tagccgtgac ggcgatgggg
    38221 acggtcagcc agaaaacagc gcgccacgat gccgtgtcgg tgaggagccc tccgaggaac
    38281 gggccgatgg ccatgcccga tccgccgaac accgccaaca ggttcacggc cgtcgcccgg
    38341 tgcgccgggt catcgaagct cttaccgacg agcgcaagga tcatcggcag gagcagggcg
    38401 gcgaacacac cctgtgccgc ccggcaaacg atcagcacgg ccaggctcgg gctgagagcg
    38461 cacccgacgg aggccatggc gaaaccgagc gcgccgatct tgaacaggcg ctgcgcgcca
    38521 atgcggtctc caaggctgcc accgaacagc agcagtgagg cgagcggcaa cgtgtacgcg
    38581 tcgacgaccc aggatgctcc cccccgcctg cgcgtccagt tgctgcgcaa tcgtcggcaa
    38641 cgtcagattc agcgcgctgc cgtcgagcgt gatcacggcc cagccgagat agatcgccca
    38701 acctcgcgcc gaccacgccg tgcgcgccgg cgcaaccgag accgttgtca tgcccgcgcc
    38761 gctgtcagca gcgccagtgc gccctcgacc gcatcatcga aggcggacgt gtcctgcgcg
    38821 gccctcgcga ggacgtatgc accctgcagg atggcagcga tcgccgtgcc cgtgcggccc
    38881 gcatccaggt ctgcgcgcaa ttcaccggcg tcgaccccgc gctggatcac ctccgcgagc
    38941 tgcgaacgaa cccaggccag catgtcgcga acctcggccc acagggccgg ggactcaaga
    39001 atgtccgcat cctgggcaag catgccgaac tgacagccct taagcacttc ccgttctcgc
    39061 gtcaagtacg cccggacctg ccccaccgcg ctaggggcgg aagccagatc agacgcgatc
    39121 tgtgaccgca tctcgtccgc gttccgctcg atcgccgccc tcgcaagcga ctccttacct
    39181 cggaagtgat ggtacatgct gccctgacca gctccagacg catcgaggat cgcccgcgga
    39241 ctcgtcgctc cgtaccctcg ctcccacaac aattcgcggg tcgcttcgat caaatcatca
    39301 cgtgtgctca cgtcagtagt gtacatacca gtagttacag aactagaacg attgccctcg
    39361 gcgaatgggg cagcagggtt tatcgccacg aggaccttag gctggtgcac gcgtaagcct
    39421 cagcgtgcaa ggaggaactc gatggcagca ggagactggg tcaaggttga tgcgtggctc
    39481 gtcgccccca gcgaggccgg ggagtatgtc cgccgacgag aagcgctcgc gctcgcggtc
    39541 ctggccgaag cggaacgccg cggtcttcgg gcggggcgtg cagggaaggg aagcgaggac
    39601 ggtgaatatg tcatcgcttc ggacgccgcc ggcgagaccg ccgccctggt gcatctcgac
    39661 ccgatgacca cggatgaatg ggcccaggca tctgcacggg atcgcctcac cgacttcttc
    39721 gcacgacact ttgccgcatc ggcgtaacca gctaaatcct cccacccacg gtccggctcg
    39781 tcgctcacgc ccacctcatg cttagaagcg tcgatgtctg cggcatttca tatcgctcgc
    39841 aattcgagag aatctaatct agagtgaacg cgacggttca atcgtagctc tggaggagcg
    39901 gatgtcgaag gcgatcatca tcggtgcggg tactgcgggg ccggctgtgg cgcagttgtt
    39961 gacgcagcag ggttgggagg cgccggtctt cgaggcccgg tcgtcgttgg accccgctga
    40021 agggctcttc ctgaacgtcg cggtgaacgg gcggcgggtg ctgcagcggc tcggggtcgt
    40081 cgaccggttg ttgtccgatg ggcatccggc gggggtcatg gagatgtaca gcgggaaggg
    40141 caggcttctc gcaacggttc cgaacgggcc ggcgggcaag cccgcatccg gtggtgtcgt
    40201 cgtacggcga gggtggttgc agcaggtgct gtgggagagc gccgcggagg cgggcgtgca
    40261 catccacacc ggcagacgcg tcgtccgcgt cgacgagtcc acgcgtggag tgcgggtgca
    40321 tttcgacgac ggtgcggtgg aagagggcga tatcgtcatc ggcgcggatg gtgtgggatc
    40381 gcgggtgcgg cgtttcatcg atcccggcgt ccaggccgag ttcaccggcc tcatcggcgc
    40441 gggcgggctc gcccgcgtgc ccgaccttga tccgacaccc ggtgtgcagc ggttcgtctt
    40501 cgggcggcgg tcattcttcg gctacctcgt ccgcgaagac gggaccgtgt attggttcgc
    40561 caacgcgacc gcatccgagc cgccgcccca ggatcacccg gccgacgcgg cggacgtcct
    40621 cgcccagctg cgcgcgctgc atcgggatga ccccgacccc gtgcccgcga tcctgcagaa
    40681 cacggtcggc ccggtcggct cgtacccgat cttccggctc cccacggttc cgaggtggtg
    40741 gaaggggcgg gcagtcgcca tcggtgacgc ggtccacgcg accagcccgt ccgcaggtca
    40801 gggggcgtcc ctcgcgctcg aggatgccgc atccctcgcc gcggcactca gccacaaccc
    40861 cgatcacgaa cgcgcgttcg cgtcatttca agcggaccgt cagcaacggg cggagaaggt
    40921 agtcgcgtac gcggcggaga tcgaccggca gaagaagacc agcggcaacc atctcgcggt
    40981 cgcgttccgt gacctgctgc tcccgctctt cctccgcaac gcgaccaagg acacccgcta
    41041 cgactgggtg ttcgactatc acctccccac gatgaagcac tcggacaacc agcccggagg
    41101 cgctcgtgac cagtgagttc cgtcagaatc tgggctccgg gatcagagag gtgaaccccg
    41161 ccgccggctc tacgggaagg gcaggaacgc gtggccgacg cgcgttcagg gtggtgttcg
    41221 cgatcttcgc cgcggtgcat gcgctcctgg tgctcgccca gccgttcacg gcggggtgga
    41281 gcctcgagtt cggcggcggc gcgctcgact ggcatgggaa cacggcgaac gcgatcgtga
    41341 cgctcgggtt ccttcagatc atcccggggg tcctcgcgcg ctggcccggc ggggcccctg
    41401 tcccggcgcc gatcaccgcc gtgctgatct tcctcgccga agtcatgcaa ctgcagctcg
    41461 gctaccacgg cggcttcgcg ctccacctcc ccctcggcgt tgccgtcgtc ggaggcgcgg
    41521 tcgtcctcgc cgtgttgtcc ttccgatggg cgagaccacg cccatgacgg gtcccgcagt
    41581 gatctcgcgc cgtctgttct tgcgcgcggg cctcgccggc cttgccgtcg tagccgtcgg
    41641 caccctcgcc gggtgcgacc tcgtgccctc gtacactccg atggactcgt tcggtcgcgt
    41701 cgacttccgt cagcccctgc ccctcccccc gcttaacgaa gggcgcatgc aggacggcgc
    41761 ccgacggttc gagctcgtcg cacaggcagg caagagccgc atcatcgatg ccggttccac
    41821 gacgacctgg gggatcaacg gcccgcttct cggaccaacc ctccgtgtcc ggcgcggtga
    41881 tcacatcgag atggccgtgc gcaacgacct gggtgagccg accacgcttc actggcacgg
    41941 catgcatctg cccgctgccg ccgacggcgg accccaccag acgatcgcgc ccggtgagac
    42001 ctggtctccg aactgggtcg tggatcagcc cgccgcgaca ctctggtacc accctcatcc
    42061 tcatgggcag accgaacggc acgtctatcg cggactggct gggatgctca tcatcgacga
    42121 cgataacgag cgcgaactgg gcctgccccg caggtacggc gaggacgact ttcccctcat
    42181 catccaggac aagaccttca acaagagcgg ggaactgctg gagacccagc gctacgacaa
    42241 cgggatgctc ggcgacacga tcctcgtcaa cggtgcggtc tcccctatcc tcacgatcac
    42301 cgcggagcac acgcggctac gcgtcctcaa cgcctcgacc gcgcgaagct acaacatcgg
    42361 cttctccgac gaccgcgagt tcacgatgat cggcagcgat ggcggcctgc tgcgcgcccc
    42421 cgtcgccctc acacgattgc acctcactcc cggagagaga gcggagatca ttctccgttg
    42481 cgcgccggac gacgagctga cccttcgctc ctacccccac ccgcaagggc tccccaccgc
    42541 aatcgccgac gcgacgggag cgtcagaccg cctcgatatc ctgctcatcc gagccgccac
    42601 cgcactcacc ccatcctcgc cgcttcccga cgaacttgct gtgcttcccg cacttgaacc
    42661 gccaccggag agcacgtggc ggcgcttcga cctgcgcggc ttccgcatca acggtgccga
    42721 catggatcct tcgcgcatcg acgtcaccgt cgaagtcgac accaccgagc tctgggaggt
    42781 gcgcaacgca cacgacaaac cgcacaactt ccacatccac gacgtgcagt tccagatccg
    42841 gaccatcgac gggcaggcgc cgccggcaca cctcgccggg tggaaggaca ccgtctatct
    42901 gccccctcgt acgcccgtgc agctcctcat gcgattctcc gaccacaccg accggaacat
    42961 gccctacatg tatcactgcc acctgctgtg gcacgaagac aacggcatga tgggacagtt
    43021 cgtcgtcgtc gatccgggcg agaccgccgg gacgccacca cccattcggg gcgatcacgg
    43081 tggacactga ccatgcatgg cccgctgaat cctgtcccgc ccgttaggta cgattctggc
    43141 gcgtagagtg aggacacatg gacgaagccg gcaatgtaga cgacgaggac cttcccgacc
    43201 ggaacgagga gatcgccttg cacggcgctg ccatgcaaga gttcatggcc agagcagtcc
    43261 tcttccagga cgccgtcgcc cacgccgccg gactgaacag cacagatctc cagaccatcg
    43321 ggatcctgat gagcaatggc ccgctcacag ccggcgaact cgccgcccgc gtggggatca
    43381 ccaaaggcgg agcaatcaca gcagtgatcg accggctcga acgatcagga tacgtccggc
    43441 gcagccggga tctcgaggac cgccgccgcg tcgccatatc ggccgtaccc gatgcgctga
    43501 tgccacggat cggacccatc tacgcgcgca tcagccagat atggggcgaa tacctcgaca
    43561 ccctcacaac cgaccagatc cgcttcgcga ccgacatgct gcggcaggcc gcagaactga
    43621 acgccaccga aacccttcgg cttcgagcag atcacccctg atacgacatc gaacgcccga
    43681 agcgttcgac ccagcaccta ctcctcaaga attccggtgc cggaccccag tccattggtt
    43741 aggttcaatt catgccctca cctgaagctc ttctcgcgtt cgccctcgca tcggtgatcc
    43801 tcatcgcgat cccaggaccg agcgtcctct tcgtgatcgg acgctcgtta tctctgggtc
    43861 gcagcggcgg actgtggagc gtgctcggca acgaactcgg agccttggcg cccatcacgg
    43921 cagtagctct cggagtcgga acaatcgttg cgcaatcggt gatggtgttc acgatcatga
    43981 aggtcctcgg agcggcctac ctggcctatc tcggcatcca agcgatccgt caccgtcgcg
    44041 acggcatcgg tggcgtcgag ccagcgaccc ccagtcgggt atccgcacgc gtcgtactga
    44101 ggcagggatt catcgtcgga gccaccaacc ccaagacgat cgtgttcttt gtggccgcgc
    44161 tgccgcagtt cgtcgatttc catgcgggag ccgtccccat gcagatgctg atcctcggcc
    44221 tcgttttcac cttcattgcg ttcatctgcg atgcggtatg ggcgctactc gccggatcgg
    44281 cccgggcatg gttcgcccgc tcgccccgca gactcgccgc cgtgcgaggc acaggcggcg
    44341 gcatgatgat cgggctcggc ggcgtgctgc tcctcgccga taacaaggcg tagtgagacc
    44401 acgcccgagc ttctctgatg gcctcgcaag gagacgaact acatagaccg catcggcgac
    44461 atgatggaga tccgcgatcg gatcatcggt tgtcgcgtca gcgttcgcgc taaaggcgag
    44521 tcaatgtgcc cgctgtcagt cgatcacggg atccgcggtg gagactggcg tcgaccggat
    44581 ctgctccacc agcctgctcc gagccgggct cggagatagc tgattgaaga ctcggtctgg
    44641 caggtgaggc cgtgcggtgc gctcatatac ggcgggctct ccggccggca gttccctgcc
    44701 gtagaggtgg ccgatggccg gcacagcgca agcgcccgcg ctgctcgtcc gccgcggcgg
    44761 gcgccgtggt catgacgagc ctgcgttagg agtcgcgctg cagttccgca gcgacgcgct
    44821 gcgcgaagtt cggcgcgccg ctgcgtgcga gccgctcaag cagttggctg atctccaccg
    44881 gcggccgccg catcgcagcc gactgctcgg tgacgatccg acaggtggcg cccggcgaga
    44941 gatcgaactg gtcaaggagg aactcatcag tacggatcac ttcaagcccc aacggtgcca
    45001 gcacatcgtc cgggaagtcc ttcacgttct cggtgacgat gacatcggca cgtcccaaga
    45061 gggcagccgc gacaacgtgg gcatcgtcgg gatcgggcag ctcgatgcct gcgacgagcg
    45121 gctcccagcc ttcgacaaga gcgtcgtcga aggcttcgtt catcgaccgg aaccgactcc
    45181 ggaatctgct cggatcgacg tcggggtgga tgcgttcgag cgcgctgagc gcttcgtcga
    45241 gaaccttctg cgaccagagc ggccggaacg cgccggcatc tgccatgcgg agcatggtgt
    45301 cacacggcgc gagcggcacg atcacgcatg catcgaggaa cgccgtgaac cgtgccaccg
    45361 ctacgactgc ttccgagccg cggccagggc atcccggtag gcgtcagcat cgacatcgaa
    45421 cagtccgtct tcgtcagcct gccgggtgag ctcgtcgagg cggatgcggc ggtcagcgcg
    45481 cttacgatca cggtacgcca gcacgtcgtg cagacggagc ctgcgatgcc tggactcgcc
    45541 gagccgctcg aagggcagtt cgtcctcatc gagcagcctg accagagtgc tccggctgac
    45601 accaagcagc gtcgccgcct gctgggtcgt cagctgtcga tcgatgggct cgacactgac
    45661 tgcctcgccg cgctccattg cggagacgac ttgacggagc acgtcgtacg cctccatagg
    45721 aagggggatc tgctcgccgt ctggaccaag cagcatcgcg ggcgcagcgt gattctccag
    45781 gaatcgggcg agctcaagca tctcactgag gtcctggggc ggatcgacga caagtgggtc
    45841 cttcgtgcgt gcggctgtag cggtcatatg gctattatcg cccaaatcgt tcaaatcgtc
    45901 catatcgatc atgatcgctt gaggtcgagg gtaggagctc ggcggcgaga cgtcggaggt
    45961 gccccgtagg cttcggccat gcgcaccacg ttcgacgccg aggcaaacgc cgcctacatc
    46021 cagctcgtgc ccgagatcac tccgggacgc tcggtgcgca acgagatcgt cgagaccgcc
    46081 ggcggcacgg tggtgctgga cttcgaccgc gcaggggtac tcctcggcgt cgaggtcctc
    46141 ggcgcggaga agctcctgga cggagagttc ctcgccggcg ctgcgcggat cgaccagccg
    46201 agcggatcgc cgtctagccg gtgacgtcgt gagcgagtac gttctgggct ctggtccgtc
    46261 aggaccgttg gggtagcgcc ggagcgtcca tcctccaacc ggtgaaatac acgtgctcgg
    46321 tcgcgggcgg atagacagca ctgtcggtgg tagcggcagt tgtgctggag ggtaggacgc
    46381 agcgacctcg gccgatgtct tcgcagacgt gagctcgctc gacgacccag tccgcaaatg
    46441 gcagtctgac acccttgccg agaggcaccg tgtactcgct tccgcttcgc cccgcgcgga
    46501 acccttccgg cagcatgccg tccccctgga gcctgttgca cgaccggcag atcatcgcca
    46561 ggtacgtgac accagccgcc tctgtgcgcc tcatctgcag caccacagcg ggcgggagat
    46621 ctgcggcgcg aagctcgcgg aggacgtgtt cttcgagagc cttctcatgt cgttcagcgg
    46681 cgtggagctc ccacggcgtc gaccactcca gcgtcgcggt ctggccacag cgggactcga
    46741 gctcaagagc ggtcacggtc cagagagtcc cccacttgct gcagtgccag cacttgacca
    46801 tctgcgtttg gacggcagcc cgggtgacga tgagctcggc aataggccga atgccgccgc
    46861 cgaggagccg cttgatgccg tccgcaagat cgaatgattg agagccgtgc aggatcggga
    46921 gttgcatctc aagggagccg acgctgccgt cgatggcgta atgcgggacc gcgcctgctg
    46981 atatgacgtt ccttggtcct gccagccaca cgcgctcgag accagtggcc tcgtatcgct
    47041 tctggcgtcg ctcgaaatcg gcgtccgtct gcggcgacca ctgcgcttcg atcgcgagcc
    47101 gccgattgcc attcgagaca agcacatcag cgatccacga ccggtccggt gcagggcatt
    47161 cgacagtggc gtcccagccg atctcccgcg cagcacgcgc tacgaccgct ttggtagcca
    47221 ggtgctcctg cgactctggt ccgccttcat gaagcaggca gtcagtgccg ggcttgtgcg
    47281 cgaagaaccg tgttccgagc ttgctcgtct tcgggacacc ctgttggccg cacatcatcg
    47341 tcagacccgc ggcacggtag ccggtcttga gctgcgtcca ctcatcctct gacatgctcc
    47401 atgactcgac gcgttcaccg cggtaggtcg cagacagtgg catgggcgaa gagtagcccc
    47461 tggcgcggac atctggcttc gaggtcaaac cgggtccgct gcgaccgatg ggggcagaaa
    47521 gtcctgatca gcgcacgctg gtctgcaccg gctcgagcaa gaacaactga ccgtcgatcg
    47581 agcccactcg ggcgatgacg tgaacgttgg tgccgacgcc gatcgtgtcc ggaacatcgc
    47641 cagtcaaatg cagatcgttc gtgatgttca cgtcacggaa ctggaagctc gggccgccgt
    47701 tcgaatgggt ctcgctgtag tcgccgttga caatcatgat gtcgtagcgg gtggtgtagc
    47761 cctcgtgctt gttcatcgcc ccgatcgagc cgtcgaactc gatgagctgg cctgagtatt
    47821 gcgtggcgaa ggcctctacc gttgggccgt cctgggggcc cgtgagcaga gccgcaaggt
    47881 cggcgttgtt ctcggcggtg agaggggtct cctcggcggg agccgtcgcg acgggctcct
    47941 cgggcttcgt cgaaggctcg tctcgcggcg cgacagcttc ggcgctgggg gtgctcgccg
    48001 gtccagcgtc gccgtccttg tcgccgccgg tgatggccgc catgatgccg atgaagatgc
    48061 ccaggaccac gagcacgccg ccgatgatcg cgaacagctt ccactggacc tttggcttgg
    48121 ggcgtcggaa gacgagctca gtccgaagct tgccggcgtt ctgggtgact agctcccagc
    48181 cgtccttctc ccacttcgag atcatgagct tctcggtgcc gcgcaccgtc tggacgttcc
    48241 tggtctcgta cttcgcggtc tcggcctcag ggcgcgcgtc agcgtccgtc atcgggttct
    48301 cccttcccag atcccgggag aacagctcac cgccgggtca tcgtgagcat atcgggcgcc
    48361 gagcacggtc cgagcaccgg acgcagcgca tcagagcggc aggagctggt cgaagatcga
    48421 cagcagatgc tctggtgaca tggcaggtcc tccggcgatc ggcccctgga ggatgttcga
    48481 gcgcgggtgc ccgggagtat ctgacagtga ctcccacccg ccgaagtcgc ggacgtaccg
    48541 ttggggtatg agctcgaaag acgaagcgcg agagttcctc acctcccgcc gcgcccacgt
    48601 cactccggct gaggccggtg taactgactg gggtgaggag cgccgagtcc cagggctgcg
    48661 acgcgaggag gtcgcggatc tcgccggggt cagcgtcggc tattacacgc gtctggaacg
    48721 cggcgagatc ggtggggcgt ccgagagcgt cctggaggct gttgcgaggg ctctgcggct
    48781 gaccgaggtc gagcgcgagc acctgttcga cctggctcgc cgggcatcct cgtcgtcacg
    48841 gtcgactgcc cggcatccgt cctcgtccgt gctcgagccg atgcgccgcc tggttgagag
    48901 catcggggtc ccggctttcg tgcagacccc ccgtctggac gcggtcgcgg cgaacggcct
    48961 gggccgcgcg ctgttcgcgc cggtcttcga agccagtgaa ccgaacttcg tccgattcaa
    49021 ctatctcgac ccgcgcgcgc gagacttcta cctcgactgg ccgctggcgc ggcgcaccgg
    49081 ggcggcgatc ctgcgccgcg aagcagggcg ggacccgctc gacgggcagc tgacagccct
    49141 catcggagag ctgtcgacgc tgagcccgct gtttcgcgac gactgggctc ggacggacgt
    49201 gcacgagcac cgcacaggcg tgaagtgctt cgtccacccg gaggtcggtc gcctcgacct
    49261 gacgttccag gtgctggaga ccccgggcga tccgacgcac cgactggtca cctatagcgc
    49321 cgagccgggt tcggacgccg cggacaagct cgcgatcctc ggtagcctcg ccgcagagcg
    49381 tcacggcgag tgacccagaa tcaccgatcg gaaccacctg cgtgcgcctg caccatcgca
    49441 cgggccgcgc tcaccttgca caaggagtga accatgaaga tcgtcgtcat cggcggatcc
    49501 ggacacatcg gcaccttcct cgtcccgcgc ctcgtgcgcg ccgggcacga agtggtgagc
    49561 atgacgcgct cgggccgcaa ccggtacgcg gcgtcgaccg agtgggacca ggtcacccac
    49621 gtcgccgtcg acagggacca gcaggacacc aaaggcatct tcgggcgcac cgtcctggcg
    49681 cagagaccag acgctgttat cgacctgctg tgcttcacca tcgagtctgc gacggcgctc
    49741 gtggaggctc tccgcggaaa cgtcgggcac ctgctgcact gcggttcact cttccgatac
    49801 ggcccgaccg tcaagtaccc catccgcgaa ggcggaggga ccgaggcgat cgacgagtac
    49861 ggccgccaga aggaggccat tgcccggatg ctcatcgcgg agtccgcgtc gccgggcggc
    49921 ctcgccacca cctcgatcca tccggggcac atcgtgggtc ccggatggct gcccatcaac
    49981 cccctcggca acaccgacgt gagcgtgtgg caccggatct cctccggcat cccgctgcgg
    50041 gtgcccagcc agggcggcga cacgatgcac catgtccatg ccgacgacgt cgcgcagctg
    50101 ttccagctcg ccgtggagaa ccgccaggcg tcaaccgggg aagacttcca cgcagtggcc
    50161 ccgtcggcgc tgaccgtccg cggctactcg ggcatcgtcg gcggatggtt tggtcagccc
    50221 ggagagatcg agccgatcac ctgggagcag tatcgcgcgg agacgaccga agcgttcgcc
    50281 cagcacagct gggaccacct acaccgcagc cactacctca gcatcgacaa ggcgaaatcc
    50341 ctgctcggct acgccccggc ctacgagccg gagcaggcgg tcttggagtc tgtccgctgg
    50401 ctcattgaga acgaccaact cgatgtggcg agaccgctct cggtctgacc aagaacgccc
    50461 ccggtcaatc gatacgacgc gccgaaagcg attgcgttca gcggggagat ggtttcgctg
    50521 tcggttcgcg cctgacaggt ctggcaaatc gaggacacct cgcgagacgc tgcgtcgcgg
    50581 tcagtgcttc gcttctgacc ggttgtggtc gtggtgcatc cgtagagtga cctgtgagag
    50641 cgaccggtcg ctcgtagcca gcaccgatgg gagcacctcg tgatcacctt caccgacttc
    50701 ctgcccgttc cggagccgca gctcacgaag gtgaagttca acatgcgctc cggcgacggc
    50761 agcggcgagg ccttggactt cctcatggac gaccacgagc gctggctcaa tttcggccgc
    50821 tggcgcacga agcagacgaa caacaacatg ggcggtgcgc gctacctgct ggccttcgcg
    50881 cagtactacc cctacgggcc gcagtacttc atcttcggcg gcgcgtacga ggtgaccgag
    50941 atcaagcccg atcggatcga cgaccatggc tacgagctga cacccctccc tttgcacgag
    51001 gacttcatca agcgcctcat catcaagctc gagcggccca tcggtcggac tccatatctg
    51061 cgccactacg tgggattgca ggagagcacg ctcaatccgg aggtctacga gctcgcaccg
    51121 gacatcaagc tcgggacctt ccccgggtac cagaacgtcc ggctgcggca tcacgagctg
    51181 cagaggatca tcgccggcga cgagcccagc tggagggacg cgctctccag cgtgaagggc
    51241 gtctacgtca tcaccgacct cagctgcggg cggctatacg tcgggtcagc ctcgggggag
    51301 gcgaacgggc tgtggcagcg ctggtccggc tacgcacacc tgaagaacct caccggtggc
    51361 aacgtcgaac tcgagcagct ccgccggaac ctcggcgacg cgcacatcgt ggagaacttc
    51421 cagtactcga tcctggagat cttcgacccc aagacccggg ccgacacgat cctggcgcgc
    51481 gagtcgttct ggaagaacgc gctggacagc cgcgcgcacg ggatgaatct gaactgacac
    51541 tcgcgttcgt gtcaggttaa gcgcccgcgg gatcgcgaaa gccgttctcg atcgatgatc
    51601 ggagcgactg acacgttcga agtgtaaacg cgttcgggtt gctgatcgcg gtggcagggc
    51661 tgatgctctt tcacaccgcc ctgatcatca ccgtgcgtgc gaatcggaga tcgcgggtcc
    51721 cgttctggag gaacgctgag aagatcccag ctggaaccgt cgctatgagg gtgatcggag
    51781 cagggttcat cgtgctcggc atcgtactgc tcggtgtgaa tgcctggtgg gtgccgttcg
    51841 cgatcgtcct cgccggcccc gttgtggcgc tcatggtgat tgcgatccac aaccgttcgg
    51901 tcgctactgc tcgctgacac gaaaaggctc gagagcgtgg atgcgcattt ggtcacgtct
    51961 gcaggccgac atcgatggga actcagaagc gcgagcgcgt gtccgcggca cggtaacggc
    52021 cgattcgtag tctttgagag cgctgtagcc tgacctcgtg accgaaaccc gaccggcaat
    52081 tagcatcgaa ggcacggcgt tcgagatcgc cgtaattgag acgatgaccc cagccgagtt
    52141 cggtatcgcc ctgatcactg cgatccattg tgggggccgc aagcgggagt ggtcattcgc
    52201 ggacctcgta accgtcatcg aatcgcggcg ccctgtagcc cggagagtcg atggtgtgcg
    52261 aatcgatgac gaatacagca ggttagacat tcgacaaagg gttgccgatg gtgcgggatg
    52321 gctaatctct cgtggcctca tcggccccgc atcgaacagc tccggaagca ccggtgaatg
    52381 gcgactgact accgcaggga tcgaggcgct tgagtctggg gacgtccgca tcatcgaagc
    52441 ctcctatcgg ctgccactta gcctccaccc ccggctggga gaagcacgag cactgttcga
    52501 gcgcgggtcc ctgcagactg ccgttttctc ggcgactcac gccgtcgaag tgcgtgtccg
    52561 cgagctcgcc gggtacgggc cagaaaagta cggaaccggc ctcatgcagg aggcattcaa
    52621 cgacaagacc ggccccttgg cgatcggtga tactccggca gagcggacag cgacgatgca
    52681 gctattcctc ggtaccgtcg gggcgttcaa gaaccccacc agtcaccggg ctgtcgaata
    52741 cgatgacccg tccgaggcag ccgacatcgt ccagctgtgt gatcttcttc tcaaagtggc
    52801 ggaacgctca gcttcggtct gagacggatc aaacgccagc gctggttatg cagccatacg
    52861 gaatcgccgt tcgatagccc cggtagtggt tcctgaggga gcgtcaccct cgaagagtga
    52921 tgttcgtttt ggaacgagga ctactgtctg accctcagcg aagtcgagtg ggttggtgaa
    52981 gaatgtgatt cacacccccg atcctcgaag gacaccgaat gtctgatgcg acaagcgtcg
    53041 gcggcgcggc gaacgaactc ggtagccgtc atcgtgccgg tgttgccacg tacttggccg
    53101 tccacgggct ggtcgaccgg gaggtcctcg gacggcccgg tgcggttccg atcgccatca
    53161 gcctcgaggc ggcagaggcc gtcgatgaca tcgtctgccg gatggcggac gacacccgat
    53221 ggttctccca gagcaagcgc accgctggaa acagcgagcc gttccgggcg gccgtgcgcc
    53281 agtgggcgca acagcagctt gagcacggag atcgcgtggt actcgtcgct cgcgagctgc
    53341 gggggctgct aagcggcctc cagccctcga tcgatcaggt ggtccgcgga gatccggtgc
    53401 cggacaacgc ccgcacgaag gtcgagcagt tcgttggtgt tctccgcgat gacggcgttg
    53461 ccgacccgga gctgcttctc ggacgggtcc aactgatcga gtggagggtg gaaaccccgg
    53521 gcgacccgga ccaggaagcg ggcatagcga tgcttgccga cagcatcgtc ccgttcaacc
    53581 aggcacctcg tgcgttcgag gttctgaggg ctcgcatgct gctggcggct acccgccgcg
    53641 agactactac cgtcggcgac tggattgatt gcttggttgc cgccgatctc gatgtgtacg
    53701 cagatggcgc cgggccccgc ggcgcccgcg agcgggcccg ccagctcgcc accgccagct
    53761 atcgcgcgca tctcgcgaga cgcgcgaacc ggctcgacct gtctgccctc gtgaccgatg
    53821 tgcccgagat catcgtcgag gatgccctcg acgcgtggga agtgtcctgg gacgccagcg
    53881 ggcccaggca gatagacaaa acggatgtcc ttaacgtgat ccgtgccacc ccacgtgtcg
    53941 tcatcgtcgg cctgcccggc atgggtaagt cagagctcat gcgacagctc gccgcgaggc
    54001 tcgcacagga gccgctggcg ccggttccta tccatatcga ccttcggaac attgccagcg
    54061 ctgtgcgctc cgccgacgat ctcaccttgg acctgctgct cgagctgcca tcccggtcag
    54121 tgaccggcca ggatgctggt gttctcaagt ctgctctgac cgacgccgtc acccgaggtg
    54181 acgcgattct gcagatcgac gggctcgatg aagctcagcg ggctcgtggc caagtcgccg
    54241 ccggactggc gcgtctgctc gccgagcttc ctcccgcgac cgggctcact cttaccaccc
    54301 gcgacagcgc cctcgcatcg gctgagcaac tccacctccc gttagtgcgc ctcgagcctc
    54361 caggcaacct gcgcgacgcg atgctgacgc tgatcaccga actcgcacct caacatggcg
    54421 ccgccgactc taccgagtgg atcgccggta agaacgatga gctgatcgcc gcattcggat
    54481 cgcacaagga cgtctggagc gtaccccttc tcgccaccct tgcgacagtc cgcctcgcct
    54541 cgggccgcgg cgaggcggag tcggtcgccg ttctactcaa ccaggtgatt gaggacagcg
    54601 tgagcgaatg ggagctgcgc cgggccgccg gccgagacga gctccacacc gccgtcgact
    54661 ctcccatgct gctggaaggt ttcgccgcga tcgggcacga gctcgttggg cgcgcatcga
    54721 tttctcgcgc agaagccgca actgcggtgc gagcagcgct ggctgcgtgg caccttgcgc
    54781 cgccggtcga gggcacagtc gccgctcaga tcgtcaattt ctgggacgaa cgggtcggag
    54841 tgttcgtcga caatggcgac gctatcgtcc cgcgcagccg gcagttcgcc gaactcgccg
    54901 aggtccgctg gctgagtgac cggacaaaag cccatcgcag cgaatggctc cagaacgcgg
    54961 tccaagacga cagctacgag aatgcggtct cgctggccac gattctccat gccgatgtgc
    55021 gcgctgccct catccaccga gcgtcccgcg tctcagcgga cccgtcacgc gatcgcgccg
    55081 ccacctgggt cgcccgtcag tggcggacct ggccggccgc cgaccagagc cagcaactcc
    55141 aaatcatcga cgtgctcgcc gacgctgccg aggatcaact cccttcacct ggtcagtcga
    55201 gcggcatttt gcgagccatg gacgcgaacc gccgccgcac cgacggcgat gggtgggagt
    55261 tcgtgcttgc gctggtgaac gcacctcgca ttcccggcgt cgacgatcga cgccgggagc
    55321 gtctagccaa ccttgacgtc gaccttgaac ggcgaaccat ggtaaccgcg ctcgacggtt
    55381 tgatcgacgc gtcggcggag ggccgagcgc ccgacgcaga aaccctggcg gcggtcgagg
    55441 aaatcctcag cgtccaggtc ccggccgagc ccggttcgca gtacaacgag gagggagtgt
    55501 tggtcatcaa atcatccccg agcttcgtga ccggggtagg ggacatcgcc gagcgctgca
    55561 tcgagttcct cgatcgactc ccactcgaag cacctgatgc ggtttatgca atagcccgga
    55621 agcagtcgat acggcaatac gcccgcatca accggcgcct tgttgcgctc ggccaccggg
    55681 atccgcaacc cgccgcagcg gcgtccaggc tcttggacat gctggaggcg ttcgacgacg
    55741 accacggcgg tcttgcgtgg cttcttcgcg cactcgtcca gctgtcgccc gacatcggtt
    55801 cggagccggg agcctcgtgg cgctatccga acgtcgggcg cctgatcgac gcactcgggt
    55861 atgccgaaac cacgattcag ggactggcga gagccgcgga ggaggacgcg aacatagttc
    55921 aagggtggct cgttgcgcat atcgaggcct tcggactgga cgttgcagtc gtcgcggcgg
    55981 aggcggatca gctcatcgac gccgacgaca gtaaagctgc gagcgacagc atcatctacg
    56041 ggcgccgtca gtccgaggag gatcccgcca ccctcaccga gcccaccgct ctggcgctcg
    56101 tcccggcgct gcgttcccgc tcggaatgga tcgtcaatag cagcttccgc ctcctcacac
    56161 agacccgcta cccctcggta gcgacggctg tggcagagat tcccggccga acctcgtggc
    56221 ggacgcggtt cgaacgcaac atcgcgatcg tatacaacgc agctgacgcg gaggcggctg
    56281 tagtccgtat ggtcgcgtct gagaacacgg ctgtccggtc cgcggccgca gcgatgctag
    56341 cgctcgacta cgtgccacgt tccgacctgc tcgatggtct tcgcgacgac ggcgacggcc
    56401 aggtacggta ctacgcgggt ggcgagccgc gcaacgcaac cgaatggacc tgtagctcct
    56461 gcgcggaagt caacaccacc aatcacacat cctgttccag atgccacgtc actgcggcat
    56521 ggcgctgacc tcgtcccaat cgcacgacac caaccggctc ggtgagcgag gccgaccgca
    56581 ataggtgcgc tgctcacccg tatcaggatc accgcaagaa atctgccagg tgcggtcgtc
    56641 catccgcccg gtgcgcggcc gactggcgag cgatgccgac tggcgttggg cacgccagtc
    56701 tcttcttgcc cgcgatgccc gactatcccg cccgatgaac atgcccgtcc gggtacccgt
    56761 ccgccacgcg tgcactgtct tgagaaggaa cagccaaatg agacaaccgg gcaccggcga
    56821 ccctccagat gagttggtat ggatcaccca ccttcaaaag caactatctc tccggaaacg
    56881 gccgtcaggt ggctcaggcc ggatgccagc atcgcgtcgt tcagctcctc ggagaccgcg
    56941 cgggtgtcgc cgaccgcgag ccccgggagc agaccggcgc cgggctcggg caggcgggtc
    57001 gcacgcgcga cgaagtcggc cc
//
LOCUS       DATMSX010000413         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_129605,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000413 DATMSX010000000
VERSION     DATMSX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129605"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VN041_13230"
     CDS             <1..490
                     /locus_tag="VN041_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU30037.1"
                     /translation="MLGAHLRALGHRRVAFVHEVQRSQGYVSAGMLRADGMAEQLQLL
                     PVASAGPDRVEAEALRSAAAKGVTAFVANHDRLAAAVLKAVSAIPELADTTVTGYDDG
                     ELAEALQLTTVRQPFEETGRVALESVLERIADPSRAASRTVLEPQLVVRTSSRPARAR
                     PA"
     gene            complement(562..1776)
                     /gene="mmsB"
                     /locus_tag="VN041_13235"
     CDS             complement(562..1776)
                     /gene="mmsB"
                     /locus_tag="VN041_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HWU30038.1"
                     /translation="MSTRSETAPRTNEVLSQLTSRMRQIGIFLALIAIVVFFQVVTGG
                     KLLTAGNVANIIVQNSYILILAIGMVMIIIAGHIDLSVGSVAAFVGAMSGVMVVHWGW
                     PWWLGIIASLLVGALVGAWQGFWVAVVGIPAFIVTLAGMLLFRGLTQMVLGNTQITPF
                     PSEYRLLGGGYLFPQLFPASTSPVEWITFGLGVVTLVLFVGYEIGERSKQAKMGLETE
                     ARTWFLVRLIGGVALIAYLTYLLGMADGSRGTPVVLIVLAVLIVGYTLVMSRTVFGRH
                     IYAIGGNRHAADLSGIKSRRIDFWLFVNMGVLSALAGLVFTGRLNSAGPGAGNLFELD
                     AIAAAFIGGAAVQGGVGRIVGAITGGLIMGVLNNGMSLMGVGTDSQQFIKGMVLLLAV
                     AFDVWNKRRQRA"
     gene            complement(1763..3307)
                     /gene="mmsA"
                     /locus_tag="VN041_13240"
     CDS             complement(1763..3307)
                     /gene="mmsA"
                     /locus_tag="VN041_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWU30039.1"
                     /translation="MSTILEMRDITKEFPGVKALQDVTIEVERGQVHAICGENGAGKS
                     TLMKVLSGVYPHGTYTGDIVFEDEVAQFRDIRDSEDRGIVIIHQELALSPYLSIAENI
                     FLGNERARHGLIDWRATNLEARKLLARVGLGDDPSTPVNEIGVGKQQLVEIAKALSKE
                     VKLLILDEPTAALNDDDSAHLLDLIRHLKGQGITSIIISHKLNEIKDIADKVTIIRDG
                     RTIETLDLLSEDVSEDRIIKGMVGRDLAHRYPDRSVDLGEEVFRIEDWWVHHPADPNR
                     TVIHGANLNVRAGEVVGIAGLMGAGRTELGMSVFGRSYGTNITGRVFVRGQEVNTRTV
                     SAAIAAGIAYATEDRKQYGLNLIEDIKRNISVAALGKLARGGWVNGREEVVVAEGYRK
                     SMNIKAPSVSSVVGKLSGGNQQKVVLSKWLFADPEVLILDEPTRGIDVGAKYEIYSLI
                     NRLAAQGKGVIVISSELPELLGICDRIYALSEGRITGEVDIADATPETLMQYMTKEKE
                     ADHVHP"
     gene            complement(3381..>4430)
                     /gene="chvE"
                     /locus_tag="VN041_13245"
     CDS             complement(3381..>4430)
                     /gene="chvE"
                     /locus_tag="VN041_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU30040.1"
                     /translation="GTRGGDAPAAGGDKAASDFTVGVAMPTQQSERWIADGNNVKKTL
                     EDMGYKVDLQYANDDIPTQVSQIENMISTGADALIVASIDGTTLTDVLQQAADDKIPV
                     IAYDRLINGTKNVDYYTTFDNYQVGVQQATSLLTGLGVLDENGKDTGVTGPLNVELFA
                     GSPDDNNATFFWNGAMDTLKPYLDKGVLKVPSGQTSFEQAAILRWLPETAQKRMENIL
                     TVIGGTKLDGVLSPYDGLSIGIISALTSGGYSASALPVITGQDAEAGSVKSIVAGEQY
                     STIFKDTRELGKRAAQMVDDILKGKKPEVNDTKTYDNTVKVVPSYLLKSVIVTKDNYK
                     KVLIDSGYYTEGDLK"
BASE COUNT          670 a         1453 c         1533 g          774 t
ORIGIN      
        1 gatgctcggc gcccacctgc gtgcgctggg acaccggcgg gtggcgttcg tgcacgaggt
       61 gcagcgctca caggggtacg tgtcggccgg gatgctgcgc gcggacggga tggccgagca
      121 gctgcagctc ctgccggtgg catccgccgg gcccgatcgt gtcgaggcgg aggctctgcg
      181 ctcggcggcg gcgaagggag tcaccgcgtt cgtggcgaat cacgacaggc tggccgccgc
      241 cgtgctgaag gcggtctcgg cgatcccgga gctcgcggac acgacagtga ccggctacga
      301 cgacggcgag ctggccgagg cgctccagtt gaccaccgtg cggcagccgt tcgaggagac
      361 cggccgggtg gctctggaat ccgtgctgga gcgcatcgcc gatcccagtc gcgccgcgtc
      421 gaggaccgtg ctggagccgc aactcgtcgt gcggacgtcc tcgcggccgg cgcgcgctcg
      481 ccccgcttga gacggcgccc caacgccgag gacccggtgg atcgcgtcac gatccaccgg
      541 gccctgtgcg tctgccgcgc gtcaggcgcg ctggcggcgc ttgttccaca cgtcgaaggc
      601 cacggcaagc agcagcacca tgcccttgat gaactgctgg gagtcggtgc cgacgcccat
      661 cagcgacatg ccgttgttca gcacgcccat gatcaggcca ccggtgatcg cgccgacgat
      721 gcggccgacg ccgccctgca cggccgcgcc gccgatgaag gccgcggcga tcgcatccag
      781 ctcgaacagg ttgcccgcgc cggggccggc ggagttcagg cgaccggtga agacgaggcc
      841 ggccagggcc gacagcacgc ccatgttcac gaacagccag aagtcgatcc gccgcgactt
      901 gatgccggac aggtcggccg cgtgacggtt gccgccgatg gcgtagatgt gacgaccgaa
      961 cacggtgcgg ctcatcacca gggtgtagcc gacgatcagc acggccagca cgatcagcac
     1021 caccggggtg ccgcgcgacc cgtcggccat gccgagcagg taggtgaggt aggcgatcag
     1081 ggcgacgccg ccgatcaggc gcaccaggaa ccaggtgcga gcctcggtct ccagccccat
     1141 cttcgcctgc ttcgaccgct cgccgatctc gtagccgacg aacagcacca gggtcacgac
     1201 ccccagcccg aaggtgatcc actccaccgg cgaggtggag gcggggaaca gctgcgggaa
     1261 cagatacccg ccgccgagca gacggtactc gctcgggaac ggcgtgatct gggtgttgcc
     1321 gagcaccatc tgcgtcaggc cccggaacag cagcatgccc gccagggtga cgatgaacgc
     1381 ggggatgccg acgacggcga cccagaagcc ctgccaggcc cccaccaggg ctccgaccag
     1441 cagcgaggcg atgatgccga gccaccaggg ccagccccag tgcacgacca tcacgcccga
     1501 catggcgccg acgaacgcgg cgaccgagcc caccgacagg tcgatgtgcc cggcgatgat
     1561 gatcatcacc atgccgatcg cgaggatcag gatgtagctg ttctggacga tgatgttggc
     1621 gacgttgccc gcggtgagca gcttgccgcc ggtgacgacc tggaagaaca cgacgatggc
     1681 gatcagcgcc aggaagatgc cgatctgacg catccgcgag gtcagctgtg agagcacctc
     1741 gttcgtccgg ggtgcggtct cgctacgggt ggacatggtc ggcttccttc tccttggtca
     1801 tgtactgcat gagggtctcg ggagtggcat ccgcgatgtc gacctccccg gtgatgcgcc
     1861 cctcggagag cgcgtagatg cgatcgcaga tgcccagcag ctcgggaagc tcggacgaga
     1921 tcacgatcac gcccttgccc tgcgccgcca gccggttgat cagcgaatag atctcgtact
     1981 tcgcgccgac gtcgatgccg cgggtcggct cgtcgaggat gagcacctcg gggtcggcga
     2041 agagccactt cgacagcacg accttctgct ggttgccgcc ggagagcttg cccaccaccg
     2101 acgacaccga gggcgccttg atgttcatgc tcttgcggta gccctcggcg acgaccacct
     2161 cttcgcggcc gttcacccag ccgcccctgg cgagcttgcc gagggcggcg acggagatgt
     2221 tccgcttgat gtcctcgatc aggttgagcc cgtactgctt gcggtcctcg gtggcgtagg
     2281 cgatgcccgc ggcgatcgcg gccgacacgg tccgcgtgtt cacctcctga ccgcgcacga
     2341 agacgcggcc ggtgatgttc gtcccgtagc tgcggccgaa cacgctcatg ccgagttcgg
     2401 tccgcccggc gcccatgagc cctgcgatgc cgacgacctc tccggcgcgc acattcaggt
     2461 tcgcgccgtg gatgacggtg cggttcgggt cggcgggatg atgcacccac cagtcctcga
     2521 tccggaagac ctcctcgccg aggtcgaccg agcggtccgg gtagcggtgc gcgaggtcgc
     2581 gccccaccat gcccttgatg atgcggtcct cgctgacgtc ctcgctgagg agatcgagcg
     2641 tctcgatcgt gcggccgtcg cggatgatgg tgaccttgtc cgcgatgtcc ttgatctcgt
     2701 tgagcttgtg gctgatgatg atcgacgtga tcccctggcc cttcaggtgc cggatcaggt
     2761 cgagcaggtg cgcggagtcg tcgtcgttga gcgccgcggt cggctcgtcg aggatgagga
     2821 gtttgacctc cttcgagagc gccttggcga tctcgacgag ctgctgcttg ccgacgccga
     2881 tctcgttcac gggcgtggac gggtcatcgc ccagtccgac tcgagcgagc agcttccgtg
     2941 cctccagatt ggtggcgcgc cagtcgatca ggccgtgccg tgcccgttcg ttgccgagga
     3001 agatgttctc ggcgatcgag aggtacgggc tcagtgcgag ctcctgatgg atgatgacga
     3061 tgccgcggtc ctcggagtcg cggatgtcgc ggaactgcgc cacctcgtcc tcgaagacga
     3121 tgtcaccggt gtaggtgccg tgcgggtaca cgcccgagag caccttcatg agcgtggact
     3181 tgccggcgcc gttctcgccg cagatggcgt gcacctggcc ccgctcgact tcgatcgtca
     3241 cgtcctgcag cgccttgacg ccggggaact ccttcgtgat gtcgcgcatc tcgaggatcg
     3301 tgctcatgcg tttctcctcc cttctgcctg aggaatgccg gggtggggcc cgccgcgagg
     3361 gcgggcccca ccccgggcgg tcacttgagg tcgccctcgg tgtagtagcc gctgtcgatc
     3421 aggaccttct tgtagttgtc cttcgtgacg atgacggact tcagcaggta cgacggcacg
     3481 accttgaccg tgttgtcgta cgtcttggtg tcgttcacct cgggcttctt gcccttgagg
     3541 atgtcatcca ccatctgcgc ggcgcgcttg cccagctcgc gggtgtcctt gaagatcgtc
     3601 gagtactgct cgccggcgac gatcgacttg acgctgccgg cctcggcatc ctgacccgtg
     3661 atcaccggca gggcggaggc ggagtagccg ccggaggtga gcgccgagat gatgccgatc
     3721 gagaggccgt cgtagggcga gagcacgccg tcgagcttgg tgccgccgat gacggtgagg
     3781 atgttctcca tgcgcttctg ggcggtctcg ggcagccagc gcaggatcgc ggcctgctcg
     3841 aagctggtct gacccgacgg caccttcagc acgcccttgt ccaggtacgg cttgagggtg
     3901 tccatggcgc cgttccagaa gaacgtcgcg ttgttgtcgt cggggctgcc ggcgaacagc
     3961 tcgacgttca gcgggccggt cacgccggtg tccttgccgt tctcgtcgag cacgcccagg
     4021 ccggtgagca ggctggtcgc ctgctgcacg cccacctggt agttgtcgaa ggtcgtgtag
     4081 tagtccacgt tcttggtgcc gttgatcagg cggtcgtacg cgatcaccgg gatcttgtcg
     4141 tcggcggcct gctgtagcac gtcggtgagc gtggtgccgt cgatggatgc cacgatcagg
     4201 gcgtccgcgc ccgtggagat catgttctcg atctgggaga cctgcgtcgg gatgtcgtcg
     4261 ttcgcgtact gcagatcgac cttgtagccc atgtcctcga gggtcttctt gacgttgttg
     4321 ccgtcggcga tccagcgctc ggactgctgg gtgggcatcg cgacgccgac cgtgaagtcg
     4381 gatgccgcct tgtcgccgcc cgccgcgggg gcgtcgccgc cgcgggtgcc
//
LOCUS       DATMSX010000414         7852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_147774,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000414 DATMSX010000000
VERSION     DATMSX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147774"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VN041_13250"
     CDS             complement(<1..715)
                     /locus_tag="VN041_13250"
                     /inference="COORDINATES: protein motif:HMM:NF019735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HWU30041.1"
                     /translation="MSPLELNRRSFLAVLGAGALVTLTGAPANASTVRTATDDPLAAL
                     IAKRRIMLAGDGSAASVPELAGVLSDMSSAAAANWSAMVRPAAAPGIWADLPVAGVTG
                     TTLSGNMGVTFDRLFSLSLAYATAGCAQYQDAALAADLVDALTYLAANVYVAGRSPVG
                     NWWFWEIGVPRKAADILVLLHDVVPAEVRGSLLAAARYFTPDPNWRGRGTSFAETGAN
                     RSDKALNTALRGILDDRPDE"
     gene            complement(864..1667)
                     /locus_tag="VN041_13255"
     CDS             complement(864..1667)
                     /locus_tag="VN041_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU30042.1"
                     /translation="MLSIGAFAQIGQVTHRMLRHWDTAGLLVPAHVDEFNGYRSYDPS
                     QLDRLHRIVALRQLGFGLDEIGTILEEGLDAGRIEALLRTHRSRVEREHRIAAARLVD
                     VERRLHLIQKEKHMSRIEIIEKPLPAVRLAARTAVAAEQAEIGTIVGPAFDAVSEALS
                     RVGASLATPIAQYSSAEDGMLLTVGYEYSGPDVEGVEIVELPAAETAICGVHLGSMER
                     IPDSWAALHEEIIARGYVPSGPCRELYVRAVSEDQSDWVTELQQPVAQG"
     gene            complement(1766..3007)
                     /locus_tag="VN041_13260"
     CDS             complement(1766..3007)
                     /locus_tag="VN041_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HWU30043.1"
                     /translation="MSDVEDAIARAYRAEWAVIVAGLAGRFGDLDLAEESAAEAFATA
                     VARWPSDGVPPKPGAWLMITATRKGIDRIRRENRRTEKHTEAQSMFDVNAESEPVGVI
                     DDDRLRLIFTCCHPALAVESRLALTLRMVGGLTVAEIARAFLASESAIGQRITRAKAK
                     IKAARIPYRVPSDDDLADRVTGVLTVLFLVFNEGYLATGADTDPVRPELTAEAIRLTR
                     LVRTLLPEDGEVAGLLALMLLGEARRTARIASTGQLVPLAEQDRGSWDAALIAEGHRL
                     VRERLSAAAAGDAPGRYQILAAINAVHTSARESRDTDWSQVLALYDQLVNLDPSPIIA
                     LNRAIAVAELDGPGAALPIVDRLERDLAGYHSFHATRADLLRRLGRGEDARAAYGQAI
                     DLAGNAAEIAYLTAKRDSLPS"
     gene            complement(3004..3327)
                     /locus_tag="VN041_13265"
     CDS             complement(3004..3327)
                     /locus_tag="VN041_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWU30044.1"
                     /translation="MQYLVSVIDDRADPGSSDRRPAISEFNERLISGGYWVFAGGLAD
                     PDAATVIDNRGEHPVFTDGPFVESKEYLAGVWVWEAPDLDVALTLAAEASRICDRKLE
                     VRPFQ"
     gene            complement(3382..3741)
                     /locus_tag="VN041_13270"
     CDS             complement(3382..3741)
                     /locus_tag="VN041_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30045.1"
                     /translation="MAAMEDVLALGAELERSYLAYVRGRVKFRVGQLVYVAFSEDGRV
                     MGFAFPREERAALVGGNPRKFHLPSDADMRFNWVHADLAALEAAEARELVVDAWRMVV
                     PAKVSRAYDLAHPDGPQ"
     gene            complement(3804..4415)
                     /locus_tag="VN041_13275"
     CDS             complement(3804..4415)
                     /locus_tag="VN041_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU30046.1"
                     /translation="MKLKTITQITLDGVTQGNGGPTAEDRAQGFERGGWARGAGDETT
                     RDHIAATFQRADAFLFGRRTYDILLDFWGTIDDMKHHPIGVALNSKPKFVASRTLIEP
                     AWTNTTVLGDDLLSAVRELKSEGDGEVQVHGSSRLIQCLLENDLVDEMELITVPVVLG
                     QGARLFAETGRDIAMSLVDSRSDSRGVMIQTYRPAGRPAYATF"
     gene            complement(4597..5922)
                     /locus_tag="VN041_13280"
     CDS             complement(4597..5922)
                     /locus_tag="VN041_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30047.1"
                     /translation="MTSLWRNLQYVSWLVSDTSKGLAGALFGFAIPLIALIVTNDPAQ
                     AGIIGAAGTAVRVTLTLFGGVLADRHRRITLMVLGGSIGVALSAAFTVLALGDALTFS
                     TLLVIDVLLAARSGLFDVAGESALKDIVAPDTMGRAQAANQARDAALQLAGGPIGGAL
                     LVAGGWLVGAVMTLCHLVSAVSAAIIPRLRGADAGTGPAEPVDDVATGVETGGDAASS
                     SKPNALREIREGFAWLFSRPDLRGVLLILTIINLGINTVMTTIVYSLQQTGHSPAMIG
                     LISAGMGATMLVGALLATPLVSRIGAGVLMVAGLVALSVGSAATLFVQEPLWIVLVMA
                     IPGLTLAPLNAAMGGYFMVATPSELMGRASSASGVFAMGAMPLAPLIAGFGLTWFGRG
                     GTILLGAGLCAIAALMAVTNRGLRSLPAEAGWAAHARQFEARKTLVDTH"
     gene            5996..6580
                     /locus_tag="VN041_13285"
     CDS             5996..6580
                     /locus_tag="VN041_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30048.1"
                     /translation="MTEQQEERPSRTMTTAMLKAFANPLRRDMMRVFAKREFLRAADL
                     AEELGQPANKISFHLRVMADAGLIVEAPEHARDGRDRMWTPIRESLNVGSPEAPVADV
                     ALGNVVIAALAEDHQKLVQRVVSYALENLAGGRDGVEHGTLAIHNLRLTDAEYEQLTK
                     KLGRVISEAEAAHDRSAPDSRFWQIDIVAADESI"
     gene            complement(6588..7049)
                     /locus_tag="VN041_13290"
     CDS             complement(6588..7049)
                     /locus_tag="VN041_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU30049.1"
                     /translation="MSELTELSFTVSGRISRPVAEVYEAVTDPDRLSKYFTTGGARGR
                     LEPGAEVSWDFADFPGRFPVTVVEAEAPRRIVIEWDGNETVDDRTRVVFEFAPIDNGT
                     RTLVTITESSWRATDAGAKSAFGNCEGWTGMLAALKAWVEHGINLREGFYK"
     gene            7066..7389
                     /locus_tag="VN041_13295"
     CDS             7066..7389
                     /locus_tag="VN041_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30050.1"
                     /translation="MSSRDQAPDDDDLVFKALAAPVRRRMLDALRPAELTTGELCARF
                     PELDRTTVLQHLRVLERADLVIGRRIGRERRLSLAPLPIKRIHDRWIGDYARAAVELL
                     EDLDG"
     gene            complement(7625..>7852)
                     /locus_tag="VN041_13300"
     CDS             complement(7625..>7852)
                     /locus_tag="VN041_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HWU30051.1"
                     /translation="DLLSALPAPMRELGRFRVLAVRAALASADLAEAGRLLEEGIEVP
                     DIREGELSMSELWRQAFPDRPVPPRYDFSMS"
BASE COUNT         1270 a         2647 c         2739 g         1196 t
ORIGIN      
        1 tctcgtcggg ccggtcgtcg aggatgccgc gcagggcggt gttcagagcc ttgtcgctgc
       61 ggttggcgcc ggtctcggcg aagctggtgc cgcgtccgcg ccagttcggg tcgggcgtga
      121 agtagcgggc ggcggccagc agcgagccgc gcacctcggc ggggacgacg tcatgcagca
      181 agacgaggat gtcggcggcc ttgcgcggca cgccgatctc ccagaaccac cagttgccga
      241 cgggcgagcg gcccgccacg tagacgttgg ccgccaggta ggtgagcgca tcgacgaggt
      301 cggccgccag cgcagcatcc tgatactgcg cgcagcccgc ggtcgcatat gcgagcgaca
      361 ggctgaacag gcggtcgaac gtgacgccca tgttgccgga gagcgtggtc ccggtgacgc
      421 cggcgaccgg caggtcggcc cagatcccgg gcgcggccgc cgggcgcacc atcgcgctcc
      481 agttcgccgc ggcggcgctg ctcatgtcgc tgagcacgcc ggcgagttcg gggacggatg
      541 ccgcgctgcc gtcgccggcg agcatgatcc ggcgcttggc gatgagggcg gcgagcggat
      601 cgtctgtggc cgtgcgcacg gtcgacgcgt tggcgggtgc gccggtcagg gtgaccagcg
      661 cacccgcgcc gagcacggcg aggaaggaac ggcggttgag ttcaagaggg gacatcatcg
      721 gcctccaaag ggtgggaaga ggggttggag gccaaggtat acgctttccc gccggattag
      781 tagagactat ggactaattc ggtcatccgt ccggccacgt cgcgcgggct gcctgccgcg
      841 cgacgtggat ccgtgcgggc gattcacccc tgagcgacgg gctgctgcag ctcggtgacc
      901 cagtcggact ggtcctccga gaccgcgcgc acgtacagct cgcggcacgg gcccgagggc
      961 acgtagccgc gggcgatgat ctcctcgtgc agcgccgccc agctgtcggg gatgcgctcc
     1021 atcgagccca ggtgcacgcc gcagatcgcc gtctcggcgg cgggaagctc gacgatctcc
     1081 acgccctcga cgtcggggcc ggagtactcg tagccgacgg tcagcagcat cccgtcctcg
     1141 gcggacgagt actgcgcgat cggcgtcgcc aaggaggcgc cgaccctgct gagcgcttcg
     1201 gacacggcgt cgaaggcggg cccgacgatg gtgccgatct ccgcctgctc ggcggcgacg
     1261 gccgtgcggg cggcaagacg gacggcgggc agcggcttct cgatgatctc gatgcgggac
     1321 atgtgcttct ccttctggat gagatggagc cgccgttcca cgtcgacgag tctcgcggcc
     1381 gcgatccggt gctcgcgctc cacgcgggat cggtgggtgc gcagcagcgc ttcgatgcgg
     1441 ccggcatcca gcccctcctc gaggatggtg ccgatctcgt cgagaccgaa ccccagctga
     1501 cgcagcgcca cgatccggtg cagccgatcg agctgcgacg gatcgtagga gcggtagccg
     1561 ttgaactcgt ccacatgggc gggtacgagc agcccggccg tgtcccagtg ccgcagcatg
     1621 cggtgggtca cctggccgat ctgcgcgaac gctccgatgg acaacatggt tcagttctcc
     1681 cgtctcacac cgtgtcaggg tcaagtctgg ccgacctccg tgccgccgac tccggatcag
     1741 gacggtcgcg cccgccgaag ccatgtcacg acggaaggct gtcgcgcttt gccgtgaggt
     1801 aggcgatctc ggccgcgttg cctgccagat cgatcgcctg tccgtacgcg gcgcgcgcat
     1861 cctcgccgcg gcccagtctc cgcaacaggt ccgcgcgggt ggcgtggaag gagtgatagc
     1921 cggccaggtc gcgttcgagc cgatccacga tcgggagcgc ggctccgggg ccgtcgagct
     1981 cggcgaccgc gatggcccga ttgagagcga tgatcggcga cggatcgagg ttgacgagct
     2041 gatcgtagag agcgagcacc tgcgaccagt cggtgtctcg gctttcccga gcagaggtgt
     2101 gcaccgcgtt gatcgccgcg aggatctgat agcgacctgg agcgtcaccg gcggcagcgg
     2161 cggacaggcg ctcacgcacc agccgatggc cctcggcgat gagtgcggcg tcccaggagc
     2221 cgcggtcctg ctcggcgagg gggaccagtt gccctgtgct ggcgatccga gcggtgcgcc
     2281 gggcttcgcc gaggagcatc aacgccagca gtcccgccac ctcgccgtcc tccggcagga
     2341 gcgtccgaac caggcgagtg agccggatgg cctcggccgt cagctcggga cggaccgggt
     2401 cggtgtcggc gcccgtcgcc agatagccct cgttgaagac caggaagagc accgtgagca
     2461 cgccggtgac gcggtcggcc agatcgtcgt cagacggcac tcggtaggga atgcgcgcgg
     2521 ccttgatctt ggccttggcg cgggtgatcc gctgcccgat cgcgctctcg gaggcgagga
     2581 acgcgcgggc gatctcggcc acggtcagac caccgaccat acgcagggtc agtgccagcc
     2641 ggctctccac ggccaacgcg ggatggcagc aggtgaagat caatcggagc cggtcgtcat
     2701 ctatgacgcc gaccggctcc gattctgcgt tgacgtcgaa catcgactgt gcctcggtgt
     2761 gcttctccgt gcgtcggttc tcgcggcgga ttcgatcgat gcccttgcga gtggcagtga
     2821 tcatgagcca tgcgcccggc ttcgggggca cgccgtccga cggccaacgc gcgacagcgg
     2881 tcgcgaaggc ctccgcggcg gactcttcgg cgagatccag gtcgccgaag cggccggcca
     2941 ggccggcgac gatcacggcc cactcggcgc ggtatgcccg ggcgatcgca tcctcgacgt
     3001 cactcactgg aacggtcgca cctcgagctt gcgatcgcag atcctggacg cctctgcggc
     3061 gagggtgagg gcgacgtcca gatcgggcgc ctcccacacc cagacgccgg cgaggtactc
     3121 cttcgactcg acgaaaggac catcggtgaa cacggggtgt tcaccgcggt tgtcgatcac
     3181 ggtggccgca tcggggtcgg cgagaccacc tgcgaacacc cagtagccgc ctgagatgag
     3241 tcgctcgttg aattcgctga tcgccggtcg tctgtcggag ctgccgggat cggctctgtc
     3301 gtcgatcacg gacacgaggt actgcatggg aagatcctct cttcggttcg tccagtacgg
     3361 aacatctggc gtgcgtcggc gtcactgcgg gccgtcggga tgcgcgagat cgtaagcgcg
     3421 ggagaccttc gccgggacga ccatgcgcca cgcgtcgacg acgagttccc gagcctcggc
     3481 cgcctccagg gcggcgagat ccgcatggac ccagttgaag cgcatgtccg cgtccgaggg
     3541 caggtggaac ttgcgcgggt tccctccgac gagcgccgcc cgctcctccc tgggaaaggc
     3601 gaagcccatc acgcgtccat cttcggagaa ggcgacgtac accagctgac cgacgcggaa
     3661 cttcactctc ccgcgcacgt atgcgagata cgagcgttcc agttccgcac cgagcgccag
     3721 gacgtcctcc atcgcagcca tgtcgacagc ctctcagctc ttcggctctt tcgtgcttcg
     3781 aagatcggcg gcgcgcctgt cgatcagaag gtcgcgtagg ccgggcggcc ggcgggtcgg
     3841 taggtctgga tcatcactcc gcgcgaatcc gagcgtgagt cgacgaggct catcgcgatg
     3901 tcccgtcctg tctccgcgaa gagacgcgct ccctggccca gcaccacggg gacggtgatg
     3961 agctccatct cgtcgacgag atcgttctcc agcagacact ggatgagccg gctgcttcca
     4021 tgcacctgca cctcgccgtc gccctcggac ttcagctctc tgacggcaga cagaagatcg
     4081 tcgccgagga cggtcgtgtt cgtccacgcc ggttcgatca gcgtcctgga cgccacgaac
     4141 ttcggcttcg agttcagggc gactccgatc ggatggtgct tcatgtcgtc gatcgtgccc
     4201 cagaaatcga gaaggatgtc gtacgtcctc cgcccgaaga ggaaggcatc ggcgcgctgg
     4261 aatgtcgcgg cgatgtgatc ccgggtcgtt tcgtcaccgg cgcccctcgc ccatccaccg
     4321 cgttcgaacc cctgcgcgcg gtcctcggcc gtaggtccgc cattgccctg agtcaccccg
     4381 tcgagggtga tctgcgtgat cgtcttgagt ttcatctgcg ctcttctcca tgacctcggc
     4441 atcccggagt gggaaccgct catcaactca acgaacgagg cctgcccaat ccgacatccg
     4501 agatcagaac ccggcccgga tatgacccga gggggtgccg gtggccgccc gccgcactcg
     4561 ccgtcggtgt gtactcccgc gtgaagcgct cggggctcag tgcgtgtcga cgagggtctt
     4621 gcgcgcttcg aactgccggg cgtgcgcggc ccagccggcc tcggcaggaa gtgagcgcag
     4681 gccgcggttg gtgacggcca tcagggcggc gatcgcgcag agaccggcgc cgagcaggat
     4741 cgtgccgcct cgtccgaacc aggtgaggcc gaagccggcg atcagcgggg cgagcggcat
     4801 ggcgcccatc gcgaagacgc cggacgcgct cgaggcccgt cccatcagct cgctcggggt
     4861 cgcgaccatg aagtagccgc ccatcgcggc attcagcggg gcgagggtca gcccggggat
     4921 cgccatcacg agcacgatcc agagcggctc ctgcacgaac agcgtcgccg ccgagccgac
     4981 actcagggcg accagcccgg ccaccatcag cacaccggcg ccgatgcgcg agacgagggg
     5041 agtggcgagg agggcgccga cgagcatggt cgcgcccatc ccggcgctga tcaggccgat
     5101 catcgcgggg gagtgcccgg tctgctgcag cgaatagacg atcgtggtca tcaccgtgtt
     5161 gatcccgagg ttgatgatcg tcaggatcag cagcacgccg cgcaggtcgg gccgcgagaa
     5221 cagccaggcg aatccctccc ggatctcgcg gagggcgttc ggcttggagg aggatgccgc
     5281 atccccaccg gtctccacgc ccgtcgcgac gtcgtcgacg ggttcagccg gcccggtgcc
     5341 ggcatccgcc ccgcgcaatc gagggatgat cgcggccgac acggccgaga ccagatggca
     5401 gagcgtcatg acggcgccca cgagccaccc gccggccacg agcagggcgc cgccgatcgg
     5461 gccgccggcc agttgcagcg cggcatccct ggcctggttc gcggcctggg cgcggcccat
     5521 ggtgtcgggg gccacgatgt ccttcagcgc gctctcgccg gcgacgtcga acagcccgga
     5581 gcgcgccgcc agcagcacgt cgatcaccag cagcgtggag aacgtgagcg cgtccccgag
     5641 ggcgagcacg gtgaaggcgg cggagagcgc gacgccgatc gacccgccca gcaccatgag
     5701 cgtgatgcgg cggtgacggt cggccagcac gccgccgaac agcgtcagcg tcacgcgcac
     5761 agcggttccg gcggcgccga tgattccggc ttgagcgggg tcgttggtga cgatcagggc
     5821 gatgagaggg atcgcgaagc cgaacagtgc gccggcgagc cccttgctcg tgtcgctgac
     5881 gagccaggac acgtactgca ggttgcgcca caacgaggtc atgacgctca atctaaccat
     5941 gcaactaaac ttgcgcaagt aaagtcgcgc aaattcatct ggatatactg cgggcatgac
     6001 cgagcagcag gaggagcgtc cttcgcggac catgaccacg gcgatgctca aggcgttcgc
     6061 caacccgctg cggcgcgaca tgatgcgggt gttcgcgaag cgcgagttcc tccgcgccgc
     6121 cgatctcgcc gaggagctcg gtcagcccgc caacaagatc agcttccacc tgagggtcat
     6181 ggcggatgcc gggctcatcg tcgaggcgcc cgagcacgcc cgggacggtc gggaccggat
     6241 gtggacgccc atccgggagt cgctgaacgt gggctccccc gaggcgccgg tcgccgacgt
     6301 cgcgctcggg aacgtcgtga tcgcggcgct cgccgaggac catcagaagc tggtccagcg
     6361 ggtcgtgtcg tatgccctgg agaacctcgc gggcgggcgc gacggcgtcg agcacggcac
     6421 gctcgccatc cacaatcttc gcctcaccga cgcggagtac gagcagctca ccaagaagct
     6481 ggggcgcgtg atctcggagg ccgaggcggc gcacgaccgc tccgccccgg actcgcggtt
     6541 ctggcagatc gacatcgtcg ccgccgacga gtcgatctga ggcgggctta cttgtagaag
     6601 ccctcgcgca ggttgatgcc gtgctcgacc caggccttca gtgcggcgag catgccggtc
     6661 cagccttcgc agttgccgaa ggcgctcttc gcgccggcat ccgtcgcgcg ccacgagctc
     6721 tcggtgatcg tcaccagcgt gcgcgtgcca ttgtcgatcg gcgcgaactc gaagacgacg
     6781 cgagtgcgat cgtcaacggt ttcgttgccg tcccactcga tcacgatccg tcgcggggcc
     6841 tcggcctcga cgacggtcac cgggaatcgc cccgggaagt ccgcgaagtc ccagctgacc
     6901 tcggcgccgg gctcgagccg gccacgggcg ccgccggtgg tgaagtactt cgagaggcga
     6961 tccggatcgg tgaccgcctc gtagacctcc gcgacagggc gggagatgcg gcccgacacg
     7021 gtgaacgaca gttcggtgag ctcagacatg ttataagatt acaacatgtc atcgagagat
     7081 caagcccccg acgatgacga cctcgtgttc aaggcgctcg ccgcgccggt cagaaggcgg
     7141 atgctcgacg cgctgcgccc cgcggaactg acgaccggtg aactgtgcgc ccgcttcccc
     7201 gagctggacc gcacgaccgt gctgcagcac ctgcgcgtgc tggagcgcgc cgacctggtg
     7261 atcggccgtc gcatcgggcg ggagcggcgg ctgtcgttgg caccgctgcc catcaagcgc
     7321 atccacgacc gctggatcgg cgactacgcc cgcgctgcgg tggagctgct cgaggatctc
     7381 gacggctgac atcacgggca tccggaacgc ccctcgcgac ctccagatcg catttcgtcc
     7441 cggattcagc cgccttccgc tgtccaggtc gcacttcgtc tcggtaggcc cgacggaaag
     7501 cgggatgaaa tgcgacctga agcgccgtcg aggacgctga acgggacgta ttgcgatctg
     7561 gacattcgag ctcccgccgt tgcaggcctg ccctgcgagt gcgaggtggc agaagcgcct
     7621 ccgctcagct catcgagaag tcgtaccgcg gcggcacagg gcggtcgggg aacgcctgcc
     7681 gccacaactc cgacatgctc agctcgccct ctcggatgtc gggcacctcg atgccctcct
     7741 cgagcaggcg gccggcttcc gcgaggtcgg cggatgccag cgctgcacga acggccagca
     7801 cgcggaagcg ccccagctcc cgcatcggcg cgggcagcgc ggagagcagg tc
//
LOCUS       DATMSX010000415         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_91792,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000415 DATMSX010000000
VERSION     DATMSX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91792"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VN041_13305"
     CDS             complement(<1..387)
                     /locus_tag="VN041_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30052.1"
                     /translation="MDITAQASSIGSLADETRRALYEYVAGQTEPVSRDAAADAVGIA
                     AHMARFHLDKLVEAGLLDTEFRRLSGRTGPGAGRPSKLYLRASGTISVSLPERRYDLV
                     GHLLAGAIERAAEGADLDRAVADVAHD"
     gene            complement(550..939)
                     /locus_tag="VN041_13310"
     CDS             complement(550..939)
                     /locus_tag="VN041_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30053.1"
                     /translation="MNTEPTVPLPETMSAPDAEHATLPLEHSILPSSETSDPLAEAPT
                     TASAPSPRTRWAAIVWGLCFAAIAWFGIWMLSDGERHDSVTDWLASLNPGTITAFGLL
                     AVGALVLISGLVGLIRRAQRRLAAANN"
     gene            complement(936..>1648)
                     /locus_tag="VN041_13315"
     CDS             complement(936..>1648)
                     /locus_tag="VN041_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30054.1"
                     /translation="RASNPRAGFAYVGTTIGVALVVASAVWLLTSDPGVTTGALAVLS
                     ASLVFAFGMVIAGVARRRSGFLAFLTAGTLVAGLITGGAATVEDVRFGEASLSNVRGG
                     EIRQPFGNTGIVLMSAAEQRSHPVILHKGIGYTEIFVAEGVELRLTATVGDVDVYGNR
                     MELDAEGNSVGGGNDAFTGTTRADGRTVYRETITSAVNPASPGAPSSITVVPVTIDQE
                     AGDIVITYSALTEKEAEK"
BASE COUNT          259 a          543 c          590 g          256 t
ORIGIN      
        1 gtcatgggcg acgtccgcga cggcgcggtc gagatccgcg ccctcggcgg cccgctcgat
       61 ggcgcccgcg agcaggtgac cgaccaagtc gtagcggcgc tcggggaggg acaccgagat
      121 cgtcccgctt gcgcgcaggt agagcttgga cggccggccc gcgccaggac ctgtgcggcc
      181 ggacagacgg cggaactcgg tgtccagaag tcctgcttcg acgagcttgt cgaggtgaaa
      241 gcgggccatg tgcgcggcga tgccgaccgc gtcggcggcg gcatccctgc tgaccggctc
      301 agtctgcccc gcgacgtact cgtacagggc gcgtcgagtc tcgtcggcga ggctgccgat
      361 gctcgacgcc tgtgcggtga tgtccatggt gacctctaaa gaagtgactc ttgtattcag
      421 atgccctgct tctatttttg atcctaactt cttttagaag ggcgggtcac ccggagggga
      481 ggatctcggg cctggaaaca gcagcggcgg tgcgaaacgc ctcgcaccgc cgccgtcgtt
      541 ctgcggtctt cagttgttcg cggccgcgag gcggcgctgc gcgcgccgga tcagtccgac
      601 gagcccgctg atgagcacga gtgcgcccac ggccagcagg ccgaacgccg tgatcgtgcc
      661 cggattgagg gaggcgagcc agtcggtcac gctgtcgtga cgttcgccgt cgctgagcat
      721 ccagatcccg aaccaggcga tcgcggcgaa gcacagaccc cagacgatcg cggcccagcg
      781 ggtgcgaggc gagggagcgg atgccgtggt cggcgcctcc gcgagcgggt cggaagtctc
      841 ggacgagggg agaatcgagt gctcgagtgg gagcgtcgcg tgctcggcat ccggcgcgga
      901 catggtctcg gggagcggga cggtcggctc ggtgttcact tctcggcctc cttctcagtg
      961 agcgcggaat aggtgatgac gatgtcgcct gcctcttggt cgatggtcac aggcacgacg
     1021 gtgatcgacg acggagcgcc aggcgatgcg gggtttaccg ctgaggtgat cgtctcccgg
     1081 tagaccgtgc ggccatccgc ccgcgtcgtg ccggtgaagg cgtcgttccc cccgccgacg
     1141 gagttgccct cggcgtccag ctccatccgg ttgccgtaca cgtcgacgtc gccgaccgtc
     1201 gcggtcagcc ggagctccac gccctcggcg acgaagatct cggtgtaccc gattcccttg
     1261 tgcaggatca cagggtgaga ccgctgctcg gccgcactca tcaggacgat cccggtgttg
     1321 ccgaacggct ggcggatctc gccgccgcgg acgttggaga ggctggcctc gccgaatcgc
     1381 acgtcctcga cggtcgccgc gccaccggtg atcagccccg ccaccaaggt ccccgcggtg
     1441 aggaacgcca ggaatccgct ccgtcgccgg gcgaccccgg cgatgaccat gccgaaggcg
     1501 aacaccagcg atgccgacag caccgcaagg gcgcccgtcg tgacgccggg atccgacgtc
     1561 agcagccaca cggccgaggc gacgaccaga gccacgccga tggtcgtgcc gacgtaggcg
     1621 aaaccagcgc gcgggttgga ggcgcggc
//
LOCUS       DATMSX010000416         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176748,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000416 DATMSX010000000
VERSION     DATMSX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176748"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VN041_13320"
     CDS             complement(<1..534)
                     /locus_tag="VN041_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30055.1"
                     /translation="MSGIRRVEGGIVTGRFTDHHVHLQLVDHALLAGSRLGRVIDLGG
                     NPEWVAGVARHNSGDPRASRPVSVESGSDTHDSTELWPVTIEYAGAFLTAPGGYPSDR
                     SWAPAGSVREIADARAAASAVAELATLGASCIKVVAHSEAGPVLDDDSFRAVVASAKS
                     RGLPVVAHAEGSGQAQRS"
     gene            complement(531..1385)
                     /gene="kynA"
                     /locus_tag="VN041_13325"
     CDS             complement(531..1385)
                     /gene="kynA"
                     /locus_tag="VN041_13325"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HWU30056.1"
                     /translation="MSNDNERALEEGIVTDLKDRMTYGSYLELDKLLDAQHPVSQPEH
                     HDEMLFIIQHQTTELWLKLVLHELGTARDRLAEDDLRSALKHIARIKHIQDVLTRQWS
                     VLATLTPTEYAEFRGDLGSSSGFQSVLYRCVEFALGNKNERMLSVFRDHPENLALLTR
                     EWERPTLYDEFLRLAARRGLPIPAEVLERDVRQPYRENEELVQAIREIYENPHEHWDL
                     YEACEELVDVEDNFQFWRFRHLRTVTRTIGMKKGTGGSTGVDFLRRALDLTFFPELYS
                     VRTEIGKE"
     gene            1479..>2780
                     /locus_tag="VN041_13330"
     CDS             1479..>2780
                     /locus_tag="VN041_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HWU30057.1"
                     /translation="MGDHVGVQSFSRQDRTRFRAKVGRCLDALAVMLADGTFDVAEPR
                     LGLEIELNLVDSAHDPAMSNARVLEEIANPAFQTELGRFNIEINVAPRPLDADNLRKL
                     EGALRAALNAADERARGVGCGLAMIGMLPTVDEHHFTEQWFSEDPRYGLLSQQVIAAR
                     GEDIALQLDGPALSEGGPREALDIVCDTILPEAACTSVQLHLQVPPEEFAAYWNSAQA
                     IAGVQVALAANSPFFAGRALWHETRIPMFEQATDTRSQELKNQGVRPRVWFGERWITS
                     IFDLFEEKSRYFPALLPVCTDEDPFEVLEAGGSPKLAELRMHNGTIYRWNRPIYDAQA
                     EGRHLRLENRVLPAGPSIPDVLADAALYYGLVRSLTDADRPLWTQMTFDAAEENLHSA
                     ARDGIEARMYWPEVGWVGPRELVLRRLLPLAAEGLDAYGVDA"
BASE COUNT          419 a          934 c          987 g          440 t
ORIGIN      
        1 cgatctctgc gcctggcccg agccctcggc gtgcgccacg acgggcagtc cacgggactt
       61 cgcggatgcc acgacggcgc ggaagctgtc gtcgtccagc accgggcccg cctcgctgtg
      121 cgccacgacc ttgatgcagg acgctccgag ggtggcgagc tcggcgaccg cggacgccgc
      181 ggcccgggca tccgcgatct cccgtaccga gccggccgga gcccaggagc ggtccgacgg
      241 atacccgccc ggtgcggtga ggaacgcgcc ggcgtactcg atcgtgaccg gccacaactc
      301 cgtggaatca tgcgtatctg acccggattc gacggaaacg ggccgagaag cccgcggatc
      361 tccggagttg tggcgagcca cgcccgccac ccactccgga ttcccgccca gatcgatcac
      421 gcggcccagg cgtgagcccg ccagcagggc gtggtcgacc agctgcaggt gcacgtggtg
      481 gtcggtgaag cggccggtga cgatgccgcc ctcgacgcga cggatgccgc tcattccttg
      541 ccgatctccg tgcgcacgct gtacagctcg gggaagaacg tcaggtccag tgcgcggcgc
      601 aagaagtcca cgccggtcga tccgccggtg cccttcttca tgccgatcgt gcgggtgacc
      661 gtgcgcaggt ggcggaagcg ccagaactgg aagttgtcct ccacgtcgac gagctcctcg
      721 cacgcctcgt acagatccca gtgctcgtgg gggttctcgt agatctcgcg gatcgcctgc
      781 accagctcct cgttctcgcg gtagggctgg cgcacgtccc gttcgaggac ctcggccggg
      841 atgggcaggc cgcggcgcgc cgccaggcgc aggaactcgt cgtagagcgt cggccgttcc
      901 cactcgcggg tcagcagcgc gaggttctcg gggtggtcgc ggaacacgct cagcatccgc
      961 tcgttcttgt tgccgagtgc gaactcgacg cagcggtaca gcaccgactg gaagccggag
     1021 gagctgccca ggtcgccgcg gaactccgcg tactcggtgg gggtgagggt ggcgagcacc
     1081 gaccactggc gggtcagcac gtcctggatg tgcttgatgc gcgcgatgtg cttgagggcg
     1141 gagcgcagat cgtcctcggc gaggcggtcg cgggccgtgc cgagctcgtg caggacgagc
     1201 ttgagccaga gctccgtggt ctggtgctgg atgatgaaca gcatctcgtc gtggtgctcg
     1261 ggctgggaga ccggatgctg ggcgtccagc agcttgtcga gctcgaggta cgagccgtac
     1321 gtcatccggt ccttgagatc ggtgacgatg ccctcttcga gtgcgcgctc gttgtcgttg
     1381 ctcattccgc gacgatatca ccgttgtgtc gggcgtcaca atagtgaaat caacggcgag
     1441 tatcgctttc cgcgcgagcg catcggtaat gtcggcccat gggagaccac gtcggcgtgc
     1501 agagcttctc gcggcaggat cgcactcgat tccgcgccaa ggtcgggcgg tgcctggatg
     1561 cgctcgcggt catgctcgcc gacggcacct tcgacgtcgc cgagccccga ctgggactgg
     1621 agatcgagct caacctcgtc gacagtgcgc acgacccggc catgtcgaac gcgcgggtgc
     1681 tggaggagat cgccaacccg gcattccaga ccgagctcgg ccggttcaac atcgagatca
     1741 acgtcgcccc gcggccgctc gacgcggaca atctccgcaa gctcgagggc gccctgcgcg
     1801 ccgcgctgaa cgcggcggac gagcgggcga ggggtgtcgg gtgcggcctg gcgatgatcg
     1861 ggatgctgcc cacggtcgac gagcatcact tcaccgagca gtggttctcg gaggacccgc
     1921 ggtacgggct gctcagccag caggtgatcg ccgcccgcgg agaggacatc gccctgcagc
     1981 tggacgggcc cgcgctgtcg gagggcggcc cgcgggaggc cctcgacatc gtctgcgaca
     2041 cgatcctgcc cgaggccgcg tgcacctcgg tgcagctgca cctgcaggtg ccgcccgagg
     2101 agttcgccgc gtactggaac tcggcgcagg cgatcgccgg cgtgcaggtc gcgctcgccg
     2161 ccaactcgcc gttcttcgcc ggtcgcgcgc tgtggcacga gacgcgcatc ccgatgttcg
     2221 agcaggccac cgacacccgc tcgcaggagc tgaagaacca gggcgtgcgt ccgcgggtgt
     2281 ggttcgggga gcgctggatc acctcgatct tcgacctgtt cgaggagaaa tcacgctact
     2341 tcccggcgct tctgccggtg tgcaccgacg aggacccgtt cgaggtgctc gaggccggtg
     2401 gctcgccgaa gctggccgag ctgcgcatgc acaacggcac catctaccgc tggaaccgcc
     2461 cgatctacga cgcccaggct gagggacgtc atctgcggct ggagaaccgc gtgctgcccg
     2521 cgggaccgag catcccggac gtgctggccg atgccgcgct gtactacggg ctcgtgcgca
     2581 gcctgaccga cgccgaccgg ccgctgtgga cgcagatgac cttcgacgcc gccgaggaga
     2641 atctgcattc tgccgcgcgg gacggcatcg aggcgcggat gtactggccc gaggtcggct
     2701 gggtgggccc gcgcgagctc gtgctgcgcc ggttgctccc gctcgccgcg gaggggctgg
     2761 atgcctacgg cgtcgacgcc
//
LOCUS       DATMSX010000417         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72631,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000417 DATMSX010000000
VERSION     DATMSX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72631"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1208)
                     /locus_tag="VN041_13335"
     CDS             complement(33..1208)
                     /locus_tag="VN041_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30058.1"
                     /translation="MRKNTRTAVAVTGLATIIAATLTGCGTAGGSGGGDAGPATKLGK
                     TEGQVSILAWPGYVEDGSNDPKVDWVSAFEKSSGCKVEAKTFGTSDEALNLMKTGDYD
                     VVSASGDASLRLIASGDVVEVNTDLLKNYDGIFDFLKDRDWNTVDGKHYGVPHGYGAN
                     LLMYNTETFPTAPDSWDVVFDKASANAGKVTAYDSPIYIADAAMYLMATKPELKIKNP
                     YALDQKQFDAAVSLLKEQRPNISEYWGDYLQEVQSFQSGDSVVGTTWQVIQNTLAGEG
                     ATTDVTLPKEGATGWSDTWMISSKSKNPNCAYAWLDYISSPEANAQATAYFGEAPSSQ
                     KACDYRDDCESYHAGDADYASKIWYWTTPIAKCLDGRTDVKCIDYAGWTTAWQQIRG"
     gene            complement(1246..2328)
                     /locus_tag="VN041_13340"
     CDS             complement(1246..2328)
                     /locus_tag="VN041_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30059.1"
                     /translation="MTDKNVAIRLTGLTRTFGSVTAVDGVDLEIAEGEFFSMLGPSGS
                     GKTTVLRLIAGFEQPTSGTVSLFGEDVTSTPPFDRDVNTVFQDYALFPHMTVLDNVAY
                     GLRVRGVGRRERRERARAALASVRLDHVGDRKPTQLSGGQRQRVALARATVVEPKVLL
                     LDEPLGALDMKLREQMQVELKQIQRDLGITFIFVTHDQEEALTLSDRIAVFNAGRIEQ
                     LGTPFELYEKPSSRFVADFVGTSNLFDVETSREVLGRPGHHSLRPEKLVVGPAASTEP
                     GTRSVTGSVVEAIYLGSGVRLVIDLDSGTRITALQQNTGGRVHESHGDRVHVSWREAD
                     VVVLDDAPIGEQNDTPSRTIRAVGAR"
     gene            2441..3208
                     /locus_tag="VN041_13345"
     CDS             2441..3208
                     /locus_tag="VN041_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU30060.1"
                     /translation="MTQLTAGVDPARSVVFAPLSDVGRSELVLQRLQNAITGGVLHDG
                     ERLPSESELGRRLGVAVVTVREALESLRDAGLIDTRRGRDGGSFVTFDPETARRFVDA
                     RIHGLSRLDLRDAAVQYSAIAGMAAEIAADRASEDDVAGLRELEEQADATTGTGARRA
                     VARFQLEVAALSQSPRLVREELKLQAEIGSLLWLCLRDEHVRDRSTAARVAVIDALAA
                     ADPERARSVTLQRITGAVEWLLDEKTRLELESDASAS"
     gene            3224..4009
                     /locus_tag="VN041_13350"
     CDS             3224..4009
                     /locus_tag="VN041_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWU30061.1"
                     /translation="MNGRVADHGQRTAAHVQEVFLPVFDLVELWRGEIATLFETFAAS
                     GRPLTASVLDPLMHELASPALSAPQGLITGAGFVATPGVLADAPWHLAWWLREIRGLD
                     RGDRGGIRRLEAVEDPESDEFRDYRMLEWWRVPERTGERHVTGPYVDYLCTDDYTITV
                     TVPVVHAARMVGMVGVDVHVSRIEPILLPSLRDAEGTATIVNAQGRVVVSSETRRVTG
                     ALLRDAEVHDALAVIHDGGRTSTVGGTTAISCGDTELILVQQR"
     gene            4050..4349
                     /gene="gatC"
                     /locus_tag="VN041_13355"
     CDS             4050..4349
                     /gene="gatC"
                     /locus_tag="VN041_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWU30062.1"
                     /translation="MSEITPDLVRHLGVLARIQLSDDEVTQLTGQLDAIVDNIAKVSE
                     VATPDVVATSHPIAMSNVFRPDEVGQTLTHEQALQNAPDSADGRFRVTAILGEEQ"
     gene            4346..>4823
                     /locus_tag="VN041_13360"
     CDS             4346..>4823
                     /locus_tag="VN041_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWU30063.1"
                     /translation="MSDIIRLTAAELGERLAAREISSVEATQAHLDRIAAVESDVRAF
                     LHVNENALAAAREVDTRRAAGEQLGPIAGVPLAVKDVLVTTDMPSTSGSKILEGYMSP
                     FDATVVARARAAGLVPLGKTNMDEFAMGSSTEHSAFGPTHNPWDLDRIPGGSGGGSA"
BASE COUNT          731 a         1604 c         1685 g          803 t
ORIGIN      
        1 gcccggggct gtccccgggc ggccggggtc cgtcagcctc ggatctgctg ccaggcggtg
       61 gtccacccgg cgtagtcgat gcacttcaca tcggtgcggc cgtcgaggca cttggcgatc
      121 ggcgtggtcc agtaccagat cttgctggcg tagtcggcgt ctccggcgtg gtaggactcg
      181 cagtcgtccc ggtagtcgca ggccttctgg ctggagggcg cctcaccgaa gtaggcggtc
      241 gcctgagcgt tcgcctccgg gctggagatg tagtccagcc acgcgtatgc gcagttcggg
      301 ttcttcgact tggaggagat catccaggtg tcgctccagc cggtcgctcc ctccttcgga
      361 agagtgacgt cggtggtcgc gccctcgcct gccagggtgt tctggatgac ctgccaggtg
      421 gtgccgacca cgctgtcacc ggactggaac gactgcacct cctgcaggta gtcgccccag
      481 tactcgctga tgttcgggcg ctgctccttc agcagcgaga cggcggcgtc gaactgcttc
      541 tgatccaggg cgtacgggtt cttgatcttc agctcgggct tggtggccat gaggtacatg
      601 gcggcgtcgg cgatgtagat gggggagtcg taggcggtca ccttgccggc gttcgcggat
      661 gccttgtcga agacgacgtc ccaggaatcc ggagccgtgg ggaaggtctc ggtgttgtac
      721 atcagcaggt tggcaccgta gccgtgcggc acgccgtagt gcttgccgtc gaccgtgttc
      781 cagtcgcggt ccttgaggaa gtcgaagatg ccgtcgtagt tcttcagcag gtcggtgttg
      841 acctccacga cgtcgcccga ggcgatcaga cgcagtgacg cgtcgccgga tgccgagacg
      901 acgtcgtagt cgccggtctt catcaggttg agcgcctcgt ccgaggtgcc gaacgtcttg
      961 gcctcgacct tgcagccgga agacttctcg aaggccgaca cccagtcgac cttcgggtcg
     1021 ttggagccgt cctccacata gccgggccag gcgaggatcg agacctggcc ctcggtcttg
     1081 cccagcttcg tggcgggacc ggcgtctccg ccaccggagc cgccggccgt gccgcaaccg
     1141 gtgagggtcg ccgcgatgat ggttgcgagt ccggtgaccg caaccgcggt gcgtgtgttc
     1201 tttcgcatgt gatactcctt cgttcgctgc ggatggggat gtgcgtcagc gggcgccgac
     1261 ggcacggatg gtgcgggatg gggtgtcgtt ctgctcgccg atgggcgcgt cgtcgagcac
     1321 cacgacgtcg gcctcgcgcc aggagacgtg caccctgtcg ccgtggctct cgtgcacgcg
     1381 tccaccggtg ttctgctgca gcgcggtgat gcgcgtgcca ctgtcgaggt cgatcacgag
     1441 gcggacgccg gagccgaggt agatcgcctc cacgaccgac ccggtgacgc tgcgcgtgcc
     1501 cggctcggtg gaggcggccg gaccgacgac gagcttctcc gggcggagcg agtggtggcc
     1561 ggggcgcccg agcacctcgc ggctggtctc gacatcgaag aggttcgagg ttccgacgaa
     1621 gtcggccacg aagcgggagg agggcttctc gtacagctcg aagggcgtgc ccagctgctc
     1681 gatgcgtccg gcgttgaaca cggcgatccg gtcggagagc gtcagcgcct cctcctggtc
     1741 gtgggtcacg aagatgaagg tgatcccgag atcgcgctgg atctgcttga gctccacctg
     1801 catctgctcg cgcagcttca tgtccagcgc gccgagcggc tcgtccagca gcagcacctt
     1861 gggttcgacg acggtggcgc gggcgagcgc gacccgctgg cgctgtcctc cggagagctg
     1921 ggtgggcttg cgatcgccca catgatcgag ccgcacgctc gcgagcgcgg ccctggcgcg
     1981 ctcgcgacgc tcccggcgcc cgacgccgcg gacgcgcagt ccgtaggcca cgttgtccag
     2041 caccgtcatg tgcgggaaca gcgcgtagtc ctggaacacc gtgttcacgt cgcgatcgaa
     2101 cgggggagtc gaggtgacgt cctctccgaa cagcgagacg gtgccggagg tgggctgctc
     2161 gaagccggcg atcagccgga gcaccgtggt cttccccgag ccggaggggc cgagcatcga
     2221 gaagaactcg ccctccgcga tctcgaggtc gacgccgtcg acagcggtca cgctgccgaa
     2281 tgtgcgcgtg aggccggtga gcctgatcgc gacgttcttg tcggtcatca catctccttc
     2341 gatgccaaat cctctgcaag tagatgtatt aaatcatatg agagcagatg tttgtgactc
     2401 cggcgtgtta cggtcgtctc tcagggagag aggagcaccc gtgacccagc tgacggcagg
     2461 cgtcgacccc gcgagatcag tggtgttcgc cccgctctcc gatgtgggca gatcagagct
     2521 ggtgctgcag cgtctgcaga acgcgatcac gggcggcgtc ctccatgacg gcgagcgact
     2581 gcccagtgaa tcggagctgg gacgcaggct cggagtcgcc gtggtcaccg tgcgcgaagc
     2641 tctcgagtcc ctgcgcgacg ccgggctgat cgacacccgt cgcggcaggg acggcggcag
     2701 cttcgtcacg ttcgaccccg agaccgcacg acggttcgtg gacgctcgca tccacggcct
     2761 cagcaggctc gacctgcgcg acgccgctgt gcagtactcc gcgatcgcgg gcatggccgc
     2821 cgagatcgcc gccgaccggg cgagcgagga cgacgtggcc gggctgcgcg aactggagga
     2881 gcaggccgat gccaccacag gcaccggtgc aaggcgagcg gtcgcgcggt tccagctcga
     2941 ggtggctgcg ctcagccagt cgccgcgact ggtgcgcgag gagctgaagc ttcaggccga
     3001 gatcggatcc ctgctctggc tgtgcctgcg cgacgagcac gtccgcgatc gcagcacggc
     3061 tgcgcgcgtt gcggtgatcg atgcactcgc cgcagcggat cccgagcgtg cacggagcgt
     3121 gacgctgcag cgcatcacgg gagcggtcga gtggctgctc gacgagaaga cacgactgga
     3181 actggagtcg gacgcgtccg cgtcctgacc ggaaggaacg aacatgaacg gcagagtcgc
     3241 cgatcacggg cagcgcacgg cagcccacgt ccaggaggtc ttcctcccgg tcttcgacct
     3301 cgtcgaactc tggcgcggcg agatcgccac cctgttcgag acgttcgccg cgtccgggcg
     3361 gcccctcacg gcatccgtcc tcgatccact gatgcacgag ctcgcctcgc ccgcgctgtc
     3421 ggcgccccag gggctgatca cgggcgccgg cttcgtcgcg acgcccggcg tgctggccga
     3481 cgctccgtgg catctcgcct ggtggctgcg ggagatccgc ggactcgacc gcggcgatcg
     3541 cggcggcatc cgtcgtctgg aggcggtcga ggatccggag tcggacgagt tccgcgacta
     3601 ccggatgctg gagtggtggc gcgtgcccga gcgcaccggt gaacggcacg tcaccgggcc
     3661 gtacgtcgac tacctctgca ccgacgacta cacgatcacc gtcaccgtgc ctgtcgtgca
     3721 cgccgcacgg atggtcggca tggtgggcgt cgacgtgcac gtgtcccgca tcgagcccat
     3781 cctgctgccg tcgctgcgag acgccgaggg cacggcgacg atcgtcaatg cgcagggacg
     3841 cgtggtcgtc tcgagcgaga cccggcgggt cacgggggca ctgctgaggg atgcggaagt
     3901 gcatgacgct ctcgccgtga tccacgacgg cgggcgcacg agcaccgtcg gaggcaccac
     3961 cgcgatcagc tgcggcgaca ccgagctgat cctcgtccag cagcgctgag cagttcggtg
     4021 cggcatggcc gctctcgtag actgggggcg tgtctgaaat cacccctgat cttgtgcgcc
     4081 atctcggcgt gctcgcgcgc atccagctct ccgatgacga ggtgacgcag ctgaccggtc
     4141 agctcgacgc catcgtcgac aacatcgcga aggtgtcgga ggtggcgacc ccggatgtcg
     4201 tcgccaccag ccacccgatc gcgatgagca acgtgttccg tcccgacgag gtcggtcaga
     4261 cgctcacgca cgagcaggcg ctgcagaacg ctcctgattc cgccgacggg cgcttccgcg
     4321 tgaccgcgat ccttggagaa gagcagtgag cgacatcatc cgcctgaccg cggcagagct
     4381 cggcgagagg ctcgccgcgc gcgagatctc cagcgtcgag gccacccagg cgcacctgga
     4441 ccgcatcgcc gccgtcgagt ccgacgtgcg cgccttcctg cacgtcaacg agaatgcgct
     4501 ggccgccgca cgcgaggtcg acactcgccg tgccgccggc gagcagctcg gccccatcgc
     4561 cggcgttccg ctggccgtca aggacgtgct ggtcaccacg gacatgccct ccaccagcgg
     4621 atccaagatc ctcgaggggt acatgtcgcc gttcgacgcc acggtcgtcg ctcgcgcccg
     4681 tgccgccggc ctggtcccgc tcggcaagac caacatggac gagttcgcca tggggtcctc
     4741 caccgagcac tccgcgttcg gccccaccca caacccgtgg gacctggacc gcatccccgg
     4801 cggctccggc ggcggctcgg ccg
//
LOCUS       DATMSX010000418        11457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_24988,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000418 DATMSX010000000
VERSION     DATMSX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24988"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VN041_13365"
     CDS             <1..440
                     /locus_tag="VN041_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HWU30064.1"
                     /translation="VGAGLVGIDPSRTVELAPAGRFTAPVVHSAEVEAGTPVGYGGAH
                     VTDRRTCISVLGVGYADGIPRELSREACVEIGGRRHRVVGRVSMDQIVVDTGDVSFPR
                     GSVATVFGPGGIAPTVQEWADWAGTIPHTIVTGIGARVARTVA"
     gene            437..1450
                     /locus_tag="VN041_13370"
     CDS             437..1450
                     /locus_tag="VN041_13370"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWU30065.1"
                     /translation="MTRVLVVGGGQSAEHEVSLAGAAGVAAALRETGFVVDEITIGRD
                     GCWCSGGTALGESPAQSLRGALQVMARADVVFPVLHGLNGEDGSLAALCNLAGVPVVG
                     SGVRAGAIGMDKWTTKLVAEAVGIRTARGVLVGSADIGDVEFESPVVVKPVAAGSSHG
                     VTLVTDAGELQDALWLAARFDDRILIEEVVRGREIDVSVLREAGGVRWATPPLEIHAA
                     AADGSALFDTAAKYDGSARFTTPADLPRRERTALTAAAVRMFDALGCAGVARIDFFLT
                     EDGPVLNEVNTMPGMTAHSQVPRMFQAAGVGYGELAGRLVEAALAAHAKSANTRAGVE
                     AAG"
     gene            1504..2562
                     /locus_tag="VN041_13375"
     CDS             1504..2562
                     /locus_tag="VN041_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWU30066.1"
                     /translation="MPLMTARAREGWPDVVKGVCIILVVMWHVVVKHSSDLPWEDAGG
                     VPEIWTVVSAQLLPLRMPLFFVVSGMFAASAVLAPTRARLTTRALRLALLYLLWVVIQ
                     TFALAAAGPDFDTARATDAWSLLLQLTVSPTNLWYLLALALYLIVGRVTRGIPTAVLL
                     PLGFLVSAVAAAGMLPDSGNLWQVVQNVVFFLAGLRLPELVRRFAARASWATAAGLGA
                     LYVASLVVVSLLGARQWFGVWTLLSALAVVAGISACALIDRWEPLAAPLRWIGRRTLP
                     IYVIHMIPLALLDRMLRGVEPWVYRGPVLAVIEPLLITALVIAASLGAHALLRRMRAE
                     VLFDPLLLGRFGGRAARL"
     gene            2753..3937
                     /locus_tag="VN041_13380"
     CDS             2753..3937
                     /locus_tag="VN041_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWU30067.1"
                     /translation="MRSTSQTSHSAGELFALVRDGKAHSRADLARMTGLSASTVALRV
                     DALVASGYLEEVGQGESRGGRRPRALAVKAAGSVVAGVDLGERHATIVLLDRRGEIVG
                     EAFENVSLLDGVEAVVTQVWASTRRLAEESGGMPLAGIAMSLPGPVDSRTTRLLSPTR
                     MPGWNGVDVGEVLGAVTGLPFVIENDANAMAVGEYIERDAAFEQMVFVKAGSGIGCGI
                     VVDGAVYRGFRGVAGDISHVALHDAPPVICSCGRAGCLDVVASGSAIVDALKEAGERI
                     DNLDDVLALAENAHPRATALLREAGARTGEVLATIINFFNPQALVVGGRLATADAFVA
                     GIRQALFTLCLPMSTDALQIEVSRAGALAGARGVGWELLETMLSPARIDAELRDATPP
                     SR"
     gene            4020..5507
                     /locus_tag="VN041_13385"
     CDS             4020..5507
                     /locus_tag="VN041_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HWU30068.1"
                     /translation="MSETASLPRIAIAGMGIESSTFSPHRAGDRDFLRLEGAALVNRY
                     DALREGELADRAEWLPVYHARSIPGGAVLADTYASIKKRICDGLAEIVASGDGLDGLL
                     LDIHGAMSVVGMDDAEGDLIAAIREVIGADVVVSATMDLHGNVSRTLVENVDLITCYR
                     LAPHEDAWETRDRALRNLVTTLESGTVPHRAWVRVPILLPGEKTSTRVEPAKSLYARI
                     PEIESRAGVVDAAIWIGYAWADEPRCHATVVVTGTDEAVTAAAAEELGRALWDVRDDF
                     EFVGPPGTLDEAVERALSSATTPYLISDSGDNPGAGGTGDVTWTLAQLLQRPELTGPD
                     SPVTLCASVFDRGAIDILRTHAVGDPVSVEAGARIDHGPHGPVRVEGVLHSLHEGDAD
                     AGGIAVVRVGGLHVIITEHRKAYHDVSDFTSIGLDAQNAAIIVTKIGYLEPELYDIQR
                     GWTLALTPGGVDQDLERLGHHRIQRPMFPFDVFDSDPDLTAEIVR"
     gene            5668..7479
                     /locus_tag="VN041_13390"
     CDS             5668..7479
                     /locus_tag="VN041_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU30069.1"
                     /translation="MRTQTLRRRMLAPLGAIAVAMIALTGCQAAQGGDAGSTGDKDTV
                     SYALPVGTGPNWILPLSAPDKMATHNSAIKATLWPRLFEYNGQSGDMGWDKKGSAAES
                     YEFSEDGKTITITLGDLDWSDGKPVTSRDVEFWFNLVKANAEKTGGYSKGNIPDNVTS
                     FTTVNDKTFSLTMDKVYNQDYFVGNQLSLVYPMPQHVWDKTSDDGEIGDFDRDPAGAV
                     KVFDYLFSQAEDMKTYATNPLWKTVSGPYTVKSWSDSGLVELTANKKYTGADKAQIEN
                     VRFLPFTSADAEMNVVRSGEVDYGYISASQLTNDKQFTDLGYGVEQWAGWSITYMPYN
                     FAGPHKNLFSQLYVRQALQHSIDQETISDAIWHGAATPDYGPIPQTVPSDQLSDAQAK
                     NPYPFDLKKAGQLFTDHGWKKNSDGVLECAAPGEGADQCGAGIAAGDLAEFVITTQNG
                     SQETDNTMAEIQSSLAKVGVKVTVDAKPLDTVLTQAQECKTAEGAKCTWDLVFFGTAG
                     SWYFSPYATGERLFAKDTKWNAGQYYNAEAESLIQEMMFSSDSETSKKYSELLAKDLP
                     VMWMPNPVYQVSVVRKGLEIGTQDPGGNFMPQRWSWK"
     gene            7504..8517
                     /locus_tag="VN041_13395"
     CDS             7504..8517
                     /locus_tag="VN041_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30070.1"
                     /translation="MSVVDAAQPTAAKKKSDSRFWWYLLRRVGQGLIVILLVTLIVFA
                     LLHLAMPDGPATGILGMQATQAQIDAFNKAHGFDLPVWQQYLNFLGQIATGDLGDSFK
                     LNRPVSDAIAQRLPKTLILALISMLVALIIAIPMGIFQAVRRGRTSDYVLTTWNFIVY
                     STPSFFLGLILVIVFSQSLRILPAQAPQGESVAAVLANPGGLVLPVLTAALSIIATFS
                     RYMRSSTIDNLSEDYVRTARAKGTPVAVVVARHVVRNSLTPVIAMLGYYLPVMFGGMI
                     VVESLFNYPGMGLLFWTSAQTSDYPVLLGCILIIAVATVIGSLLADIIQALLDPRTRE
                     ELS"
     gene            8514..9410
                     /locus_tag="VN041_13400"
     CDS             8514..9410
                     /locus_tag="VN041_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30071.1"
                     /translation="MSTPSPTSTVALATRTLSPRQRGVRRFLRNRLAVFGIVTIALFL
                     LFCFVGPLLYPTDQVHTDLRSTMLPPGSPGHPLGTDDVGYDILGRLMAGGQISILIGL
                     AAGVLATVIGTLWGAIAGYVGGIVDTVMMRIVDAAIAIPATFLLLILSAVLRPSVPLM
                     ICVIGLVSWLVPARLVRAESISLKSREYVLALRAMGGSHSRAVLKHVVPNTVGTIVVN
                     ATFQVADAILLVAYISFLGMGLQKPATDWGAMLTSGMTYTYAGAWWLIFPAGFCIVLT
                     VCAFNSIGDGLRDFFDVKGRAA"
     gene            9407..11068
                     /locus_tag="VN041_13405"
     CDS             9407..11068
                     /locus_tag="VN041_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30072.1"
                     /translation="MSDALLSITGLAVSFRTEDGPVEAVKGIDLEVRAGEVLALVGES
                     GSGKSVTAMAVLGLLPRTASVTGSIRLTGRELLGLDEDAMNSVRGGEISMVFQEPMTA
                     LNPSMRIGDQITEALHNHRKTSKDAAWARAVELLARVGIPDPERRAKSYPHELSGGQR
                     QRVVIAIALACEPQLIIADEPTTALDVTVQAEILDLIRALAAESGTAFLLVTHNMGVV
                     ADIADRVAVMFRGTMVEVGTTRDVLTAPREDYTKKLLGAVPTLPDAPSAVRADAAEQT
                     PAPVLDIVSAAVTYRRGGRSFKALDGVSLSIAPGEIVGLVGESGSGKSTLGRAALGLL
                     PLSDGTMTLFGEQIGRGGVSRRRERELRGRVGAIFQDPGSSLDPRMTVGEAIAEPMLV
                     QRRRFPTSPAQRRARVAELLDAVELPAGYATRYPHELSGGQRQRIGLARAIALEPELI
                     IADEPTSALDVSVQAAVLDVLRDLQQKMHFACLFISHDLAVVHEISDRVAVMLKGEIV
                     ETGSADDVLLSPQHPYSRRLLGSVPVPDPAKQALRREARAAALHA"
     gene            11065..>11457
                     /locus_tag="VN041_13410"
     CDS             11065..>11457
                     /locus_tag="VN041_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU30073.1"
                     /translation="MTSPAVLGVDVGGSSVKALLVDARSPLSTPPEPLFRDRMTTPLA
                     DPVDGLAEIVREVRGERSLQRVVLSIPGLVDQANGVVIRSTNIPSLDGCHLGERLGAL
                     LGVPVDVVNDGHAAAVAEATWGAGAGSDD"
BASE COUNT         1713 a         3903 c         4030 g         1811 t
ORIGIN      
        1 gggtcggcgc ggggctggtc ggcatcgacc cgtcccgcac ggtcgagttg gcacccgccg
       61 ggcggttcac ggccccggtc gtgcacagcg cggaggtcga ggcgggcacc ccggtcgggt
      121 acgggggcgc gcacgtcacg gaccggcgca catgcatctc ggtgctgggg gtcggctacg
      181 ccgacggcat accgagggag ctgtcccgcg aggcgtgcgt ggagatcggc ggcaggcggc
      241 atcgcgtcgt cgggagggtg tcgatggacc agatcgtcgt cgacaccggc gatgtgtcgt
      301 ttccccgcgg gagcgttgcg actgtgttcg gacccggcgg catcgccccg accgtgcagg
      361 agtgggccga ctgggcgggg accatcccgc acacgatcgt caccggcatc ggtgcgcgcg
      421 tggcgaggac cgtcgcatga cccgggtgct ggtggtcggc ggcgggcaga gcgcggagca
      481 cgaggtgtcg ctggccggtg cggccggtgt cgccgcggca ctgcgcgaga ccggattcgt
      541 cgtggacgag atcacgatcg gcagggacgg atgctggtgc tccggtggca ccgcgctggg
      601 cgagtcgccc gcgcagtccc tgcgcggtgc gctgcaggtg atggcgcgcg ccgacgtcgt
      661 attccccgtg ctgcatggac tgaacggcga ggatggctcg ctggccgcgc tgtgcaacct
      721 ggccggcgtg ccggtggtgg gatcgggcgt gcgggcgggc gcgatcggga tggacaagtg
      781 gaccaccaag ctggtcgccg aagccgtcgg catccgcacc gcacgaggag tactggtcgg
      841 cagcgccgac atcggcgatg tggagttcga gagcccggtg gtcgtcaagc cggtcgcggc
      901 cgggtcgagt cacggggtga cgctggtcac ggatgccggt gaactgcagg acgcgctgtg
      961 gctcgcggcg cggttcgacg accgcatcct gatcgaggag gtcgtgcgcg ggcgagagat
     1021 cgacgtctcg gtgctgcgcg aagccggagg cgtgcgctgg gcgacgccgc cgctggagat
     1081 ccacgccgcc gcggcggacg gctccgcgct gttcgacacc gcggcgaagt acgacggctc
     1141 cgcccgcttc acgacgcccg ccgacctgcc caggcgggag cgcacggcgc tcaccgcggc
     1201 ggccgtgcgg atgttcgatg cgctgggctg cgcgggcgtc gcccggatcg acttcttcct
     1261 cacggaggac ggccctgtgc tcaacgaggt gaacaccatg ccgggaatga cggcgcactc
     1321 gcaggtgccg cgcatgttcc aggctgcggg agtcggctac ggcgaactgg ccgggcggct
     1381 ggtggaggcg gcgctggccg cgcatgcgaa gtccgcgaac acgcgggccg gggtggaggc
     1441 cgcaggatga cggccgggag cgagacccga ggcgctgcgg ccgggacgac gcaggcgggg
     1501 accatgcctc tcatgaccgc ccgggcgagg gagggatggc cggacgtggt caagggcgtg
     1561 tgcatcatcc tcgtggtgat gtggcacgtc gtcgtcaagc acagctcgga tctgccgtgg
     1621 gaggacgcgg gcggcgtccc ggagatctgg accgtggtca gcgcgcagct gctgccgctg
     1681 cggatgccgc tgttcttcgt ggtctcgggc atgttcgcgg cgagtgcggt tctcgcgccc
     1741 acgcgcgcac ggctcaccac ccgggcgctg cggctcgcac tgctgtacct gctctgggtc
     1801 gtcatccaga ccttcgcgct cgccgcggcc gggccggact tcgacaccgc gcgggcgacg
     1861 gatgcctgga gcctgctcct gcagctgacg gtcagcccga ccaatctctg gtacctgctc
     1921 gcgctggcgc tctacctgat cgtgggcaga gtcactcgcg gcatccccac ggccgtgctg
     1981 cttcctctcg gcttcctcgt gtcggcggtc gcggcggccg ggatgctgcc cgacagcggc
     2041 aacctctggc aggtcgtgca gaacgtcgtc ttcttcctgg cgggcctgcg cctgccggag
     2101 ctggtgcgcc gcttcgccgc gcgcgcgagc tgggcgaccg ccgcagggct cggggcgctc
     2161 tacgtcgcat ccctggtggt ggtgtcgctg ctgggtgcgc gacagtggtt cggcgtgtgg
     2221 accctgctgt cggcgctcgc cgtggtcgcg ggcatcagcg cgtgcgccct gatcgacagg
     2281 tgggagccgc tcgcggcccc gctgcgctgg atcggccggc ggaccctgcc gatctacgtc
     2341 atccacatga tcccgctggc gctgctggat cggatgctgc gcggagtcga gccctgggtg
     2401 taccgcgggc ccgtcctcgc ggtgatcgag cccctgctga tcacagcgct cgtgatcgcc
     2461 gcgagcctgg gcgcgcacgc gctgctgcgg cggatgcggg ctgaggtgct gttcgaccct
     2521 ctgcttctgg ggcggttcgg cgggagggca gcgcgcctct gaaggatgga cgacgttctg
     2581 aaggatgccc ggcgcacgca cgtccttcag aaccgtgaac atccttcaga cgtggcgtga
     2641 cgcggcgtga cgcgaccggc tgatgcgccg cggcgacgga aaatcgacga cagatggccc
     2701 caaagtagga ctatctttgt gtgaccgtcg accgaagatg caggaggcct ccgtgcgatc
     2761 gacaagccag acctcgcatt cggcgggcga gctgttcgcg ctcgtccggg atgggaaggc
     2821 gcactcgcgc gcggatctcg cgcggatgac cggtctctcc gcatcgaccg tggcgctgcg
     2881 cgtggacgcg ctggtggcca gcggctacct ggaggaggtg ggccagggcg aatcacgcgg
     2941 gggccgccgc ccccgggccc tggcggtcaa ggctgcgggc agcgtcgtcg cgggcgtcga
     3001 cctgggcgag cggcacgcca cgatcgtgct gctcgatcgg cgtggggaga tcgtgggcga
     3061 ggcgttcgag aacgtctcgc tgctcgacgg cgtggaggcc gtcgtgacgc aggtgtgggc
     3121 gagcacgcgc cggctcgcgg aggagagcgg cgggatgccg ctggccggca tcgcgatgag
     3181 ccttcccgga ccggtggact cgcgcaccac gcggctgctg tctccgaccc ggatgcccgg
     3241 gtggaacggc gtcgacgtcg gcgaggtgct cggcgccgtc acgggcctgc ccttcgtgat
     3301 cgagaacgac gccaacgcga tggcggtggg ggagtacatc gagagggatg cggccttcga
     3361 gcagatggtg ttcgtgaagg ccgggagcgg catcggctgc gggatcgtgg tcgacggcgc
     3421 ggtgtaccgc ggcttccgcg gcgtcgccgg tgacatcagt catgtcgccc tgcacgatgc
     3481 gcctccggtg atctgctcgt gcgggcgagc gggctgcctg gacgtcgtgg ccagtggttc
     3541 cgccatcgtc gacgcactga aggaggccgg ggagcgcatc gacaacctcg acgacgtgct
     3601 ggcgctggcc gagaacgcgc atccccgcgc gacggctctg ctgcgcgagg cgggcgcgcg
     3661 caccggcgag gtgctcgcga cgatcatcaa cttcttcaat ccgcaggcgc tggtcgtcgg
     3721 cgggcgtctc gcgacggcgg atgccttcgt cgccggcatc cgtcaggccc tgttcaccct
     3781 ctgcctgccg atgtccaccg atgcgctgca gatcgaggtc agcagggccg gagcgctggc
     3841 gggggcacgc ggggtcggct gggagctgct ggagaccatg ctgtctcccg cgcgcatcga
     3901 tgcggaactg cgcgacgcca ccccgccctc tcgataaaag ttcgatccgc gaagcatctt
     3961 tgaccgattc cgattcaagc aaccggagtt caaccacctg attgaataag ctctttcgta
     4021 tgtccgaaac agcttcgctt ccccgcatcg ccatcgccgg tatgggcatc gagtccagca
     4081 ccttctcgcc gcatcgagcc ggtgatcggg acttcctgcg cctggagggc gcggcgctgg
     4141 tgaaccgcta cgacgcgctg cgcgaagggg agctcgccga tcgggccgag tggctgccgg
     4201 tctatcacgc gcgctcgatc cccggcggcg cggtgctcgc cgacacctat gcgtcgatca
     4261 agaagcgcat ctgcgacggt ctcgccgaga tcgtcgcctc gggagacggt ctggacggcc
     4321 tgctgctcga catccacggc gccatgagcg tggtggggat ggacgacgcg gagggcgacc
     4381 tgatcgccgc gatccgtgag gtgatcgggg cggacgtggt cgtctcggcg accatggacc
     4441 tgcacggcaa cgtctcgcgc acgctggtcg agaacgtcga cctcatcacc tgctaccgcc
     4501 tggcgccgca cgaggacgca tgggagacgc gggaccgcgc cctccgcaat ctcgtgacga
     4561 cgctcgagag cggcactgtg ccgcaccgcg cgtgggttcg cgtgccgatc ctgcttcccg
     4621 gcgagaagac cagcacgcgg gtggagcccg cgaagagcct gtacgcccgc atccccgaga
     4681 tcgagtcccg cgcgggtgtg gtcgacgcgg cgatctggat cggctacgcc tgggcggacg
     4741 agccgcgctg ccacgccacc gtcgtggtga ccggcaccga cgaagccgtc accgccgctg
     4801 cggccgagga gctcggtcgg gccctgtggg atgtgcgcga cgacttcgag ttcgtcggcc
     4861 ctcccggaac cctcgacgag gcggtggagc gcgccctctc ctccgcgacc accccctacc
     4921 tgatcagcga ctccggcgac aacccggggg ccggcggcac gggggacgtg acctggacgc
     4981 tcgcgcagct gctgcagcgt cccgagctca ccggacccga ctcgcccgtc acgctgtgcg
     5041 cctccgtctt cgacagggga gcgatcgaca tcctgcgcac gcacgccgtg ggcgacccgg
     5101 tctcggtgga ggccggtgca cgcatcgacc acggaccgca cggaccggtg cgggtggagg
     5161 gcgtgctgca cagcctgcac gagggcgatg cggatgccgg cggcatcgcg gtcgtgcgcg
     5221 tcggcggcct gcacgtgatc atcacggagc accgcaaggc ctatcacgac gtcagcgact
     5281 tcacctccat cgggctcgat gcgcagaacg cggcgatcat cgtcacgaag atcggctacc
     5341 tggagccgga gctgtacgac atccagcgcg gctggacgct cgccctcacg ccgggcggcg
     5401 tggaccagga cctcgagcgt ctcggccatc accgcatcca gcggccgatg ttcccgttcg
     5461 acgtcttcga cagcgacccg gacctgacgg ccgagatcgt ccgctgaccg catccgactg
     5521 cacttgtttc gtcggtcgaa acaagtgtgt tattcaaacg tgacgcatcg gctttgtttc
     5581 gcgcacttgc ttcgttcata attcaaagtg cgtgacacat cgaacgaacg accgtcgagg
     5641 tcggacgtcg cgaacaagga ggtttccatg cgcacccaga cgctgcgccg ccggatgctc
     5701 gccccgctcg gggcgatcgc ggtggcgatg atcgccctga ccggctgcca ggccgcgcag
     5761 ggcggcgacg ccggctccac cggtgacaag gacacggtga gctacgcgct gcccgtcggc
     5821 accggcccga actggatcct gccgctctcg gcgcccgaca agatggcgac ccacaacagc
     5881 gccatcaagg cgacgctgtg gccccgcctg ttcgagtaca acgggcagtc cggcgacatg
     5941 ggctgggaca agaagggatc ggccgccgag tcctacgagt tctcggagga cggcaagacc
     6001 atcaccatca cgctgggcga cctcgactgg tccgacggca agccggtgac ctcgcgcgat
     6061 gtcgagttct ggttcaacct ggtcaaggcc aacgccgaga agaccggcgg ctactccaag
     6121 ggcaacatcc ccgacaacgt gacctcgttc acgacggtca acgacaagac cttctcgctc
     6181 acgatggaca aggtctacaa ccaggactac ttcgtgggca accagctgag cctggtgtac
     6241 ccgatgccgc agcacgtgtg ggacaagacc agcgacgacg gcgagatcgg cgacttcgac
     6301 cgtgacccgg ccggcgccgt caaggtgttc gactacctgt tctcgcaggc cgaggacatg
     6361 aagacctacg ccacgaaccc gctctggaag accgtctccg gtccgtacac ggtgaagagc
     6421 tggtccgact cgggcctggt cgagctgacc gccaacaaga agtacaccgg cgccgacaag
     6481 gcgcagatcg agaacgtccg gttcctgccg ttcacctcgg cggacgccga gatgaacgtc
     6541 gtccgctccg gcgaggtcga ctacggctac atcagcgcgt cgcagttgac caacgacaag
     6601 cagttcaccg acctcggcta cggggtcgag cagtgggccg gctggtcgat cacgtacatg
     6661 ccgtacaact tcgcaggacc gcacaagaac ctgttcagcc agctctacgt gcgacaggcc
     6721 ctgcagcact cgatcgacca ggagaccatc tccgacgcga tctggcacgg cgccgccacc
     6781 ccggactacg gccccatccc gcagaccgtg ccctcggatc agctctccga cgcgcaggcg
     6841 aagaacccgt acccgttcga cctcaagaag gccggtcagc tgttcaccga ccacggctgg
     6901 aagaagaact ccgacggcgt gctcgagtgc gcggcgccgg gtgagggcgc cgaccagtgc
     6961 ggtgccggaa tcgcggccgg cgacctcgcc gagttcgtga tcaccacgca gaacggctcg
     7021 caggagaccg acaacaccat ggccgagatc cagtcgtcgc tggccaaggt cggcgtcaag
     7081 gtgaccgtcg acgccaagcc gctggacacc gtgctgacgc aggctcagga gtgcaagacc
     7141 gctgagggcg ccaagtgcac ctgggacctg gtcttcttcg gcacggcggg cagctggtac
     7201 ttcagcccgt acgccaccgg tgagcgcctc ttcgccaagg acacgaagtg gaacgccggc
     7261 cagtactaca acgccgaggc ggagtcgctc atccaggaga tgatgttctc ctccgactcc
     7321 gagacgagca agaagtactc cgagctgctc gcgaaggacc tgccggtgat gtggatgccc
     7381 aacccggtgt accaggtctc ggtcgtgcgc aagggcctcg agatcggcac ccaggacccg
     7441 ggcggcaact tcatgccgca gcgctggtcc tggaagtgac cggcagagcc tgaaggatag
     7501 gacatgagcg tggtggacgc agcgcagccg acggctgcga agaagaagag cgacagccga
     7561 ttctggtggt acctgctgag gcgggtcggc cagggtctga tcgtgatcct gctcgtgaca
     7621 ctgatcgtgt tcgcgctgct ccacctcgcc atgcccgacg ggccggccac cggcatcctg
     7681 gggatgcagg cgacccaggc ccagatcgac gccttcaaca aggcgcacgg cttcgacctg
     7741 ccggtctggc agcagtacct caacttcctc gggcagatcg cgaccggaga cctcggcgat
     7801 tcgttcaagc tgaaccggcc ggtctctgac gccatcgcgc agcgcctgcc gaagacgctg
     7861 atcctcgcgc tcatctcgat gctggtggcg ctgatcatcg cgatcccgat gggcatcttc
     7921 caggcggtcc gccggggcag gacgtcggac tacgtgctga ccacgtggaa cttcatcgtc
     7981 tactcgaccc cgtcgttctt cctcgggctc atcctcgtga tcgtcttctc gcagtcgctg
     8041 cggatcctgc ccgcgcaggc gccgcagggc gagagcgtcg ccgccgtcct ggcgaacccc
     8101 ggcggcctcg tgctccccgt cctcacggcg gcgctcagca tcatcgcgac cttctcgcgc
     8161 tacatgcggt cgtcgaccat cgacaacctc tcggaggact acgtgcgcac ggcccgcgcc
     8221 aagggcacgc cggtcgccgt ggtcgtcgcg cgccacgtgg tgcgcaactc gctcacgccg
     8281 gtcatcgcga tgctcggcta ctacctgccg gtgatgttcg gcggcatgat cgtcgtcgag
     8341 tccctcttca actacccggg catgggcctg ctgttctgga cctcggcgca gacgtccgac
     8401 tatccggtgc tgctgggctg catcctgatc atcgccgtcg cgaccgtcat cggctctctg
     8461 ctggccgaca tcatccaggc cctgctcgac ccgcgcacgc gagaggagct ctcgtgagca
     8521 ctccctcccc gacctccacc gtggctctcg ccacccgcac gctcagcccg cgccaacgcg
     8581 gcgtgcgtcg cttcctgcgc aacaggctcg cggtcttcgg catcgtgacc atcgcgctgt
     8641 tcctgctgtt ctgcttcgtc ggacctctgc tgtacccgac cgatcaggtg cacaccgacc
     8701 tgcgcagcac catgctgccg ccgggatcgc ccgggcatcc gctgggcacc gacgacgtcg
     8761 gctacgacat cctcggacgg ctcatggcgg gcgggcagat atcgatcctc atcggcctcg
     8821 cggcaggcgt gctcgccacc gtgatcggca cgctgtgggg cgcgatcgcc ggctacgtcg
     8881 gaggcatcgt cgacaccgtg atgatgcgca tcgtggacgc ggcgatcgcg attcccgcga
     8941 ccttcctgct gctgatcctc tcggcggtgc tgcggccgag cgtgcctctg atgatctgcg
     9001 tgatcggact ggtgtcctgg ctggtgcccg cccgcctggt gcgtgccgag tcgatctcgc
     9061 tgaagtcccg tgagtacgtg ctggccctgc gggccatggg cggctcgcat tcccgcgcgg
     9121 tgctcaagca cgtggtgccg aacacggtcg gcacgatcgt ggtgaacgcg acgttccagg
     9181 tcgccgacgc gatcctgctg gtcgcctaca tctcgttcct ggggatggga ctgcagaagc
     9241 ccgcgaccga ctggggcgcc atgctgacca gcggcatgac ctacacctac gccggcgcct
     9301 ggtggctgat cttccccgcc ggcttctgca tcgtgctcac cgtctgcgcc ttcaacagca
     9361 tcggcgacgg gctccgtgac ttcttcgacg tgaaggggcg tgccgcatga gcgacgcact
     9421 gctgtcgatc accggcctgg ccgtgtcgtt ccgcactgag gacggccctg tcgaggcggt
     9481 caagggcatc gacctcgagg tgagggcggg cgaggtgctc gccctggtcg gcgagtccgg
     9541 ctcgggcaag tcggtcacgg ccatggccgt gctcggtctg ctgccccgca cggcatccgt
     9601 gaccggttcg atccgcctca ccggccgcga gctgctcggt ctcgacgagg atgcgatgaa
     9661 ctcggtccgc ggcggcgaga tctcgatggt gttccaggag ccgatgacgg cgctcaaccc
     9721 gagcatgcgc atcggcgacc agatcaccga agcgctccac aaccaccgca agacgagcaa
     9781 ggatgccgcc tgggcacgcg ccgtcgagct gctcgcccgt gtcggcatcc ccgacccgga
     9841 gcgacgcgcg aagagctacc cgcacgagct ctccggcggg cagcggcagc gcgtggtgat
     9901 cgccatcgcg ctggcctgcg agccgcagct gatcatcgcc gacgagccga ccacggccct
     9961 ggacgtgacg gtgcaggccg agatcctcga cctgatccgt gcgctggcgg cggagtcggg
    10021 cacggccttc ctcctggtga cgcacaacat gggcgtggtc gccgacatcg ccgaccgggt
    10081 cgcggtgatg ttccgcggca ccatggtcga ggtggggacc actcgcgacg tgctcaccgc
    10141 gccccgtgag gactacacga agaagctgct cggggccgtg ccgacgctgc ccgacgcccc
    10201 gtcggccgtt cgcgcggatg ctgcggagca gacgcccgcg ccggtgctgg acatcgtgtc
    10261 cgcggcggtg acgtaccgtc gcggcggacg ctcgttcaag gcgctcgacg gcgtcagcct
    10321 gagcatcgcg cccggcgaga tcgtcggcct ggtcggcgag tccggttcgg gcaagtcgac
    10381 gctgggacgg gcggcactgg gcctgctgcc gctgtccgat ggcacgatga ccctcttcgg
    10441 agagcagatc gggcgcggag gcgtctcgcg acgccgggag cgcgagctgc gggggcgggt
    10501 cggtgcgatc ttccaggatc ccggttccag tctcgacccg cggatgacgg tgggcgaggc
    10561 gatcgccgag ccgatgctcg tgcagcgtcg tcgcttcccg accagccctg cacagcgcag
    10621 ggcgcgcgtg gccgagctgc tcgatgcggt ggagctgccc gcgggctacg ccacccggta
    10681 tcctcacgag ctgtccggag gacagcgcca gcgcatcgga ctggcccggg ccatcgccct
    10741 ggagccggag ctgatcatcg ccgacgagcc gacctcggcg ctcgacgtct cggtgcaggc
    10801 cgcagtgctg gacgtgttgc gcgatctgca gcagaagatg cacttcgcgt gcctgttcat
    10861 cagccatgac ctcgcggtgg tgcacgagat ctccgatcgc gtcgcggtca tgctgaaggg
    10921 cgagatcgtc gagaccggtt cggccgacga cgtgctgctg tctcctcagc acccgtacag
    10981 ccgcaggctg ctgggatcgg tcccggtgcc cgatcccgcc aagcaggcgc tgcgtcgtga
    11041 agccagggcg gcggcactgc acgcatgaca tcacctgctg tcctcggtgt cgatgtcggc
    11101 ggatccagcg tgaaggcgct gctcgtcgac gcccgatccc ctctctcgac cccgccggag
    11161 ccgctgttcc gcgaccggat gaccactccg ctggccgacc ccgtcgacgg tctcgccgag
    11221 atcgtgcggg aggtgcgcgg cgaacgctcc ctgcagcgcg tcgtgctgtc gatccccggc
    11281 ctggtcgatc aggcgaacgg cgtcgtgatc cgctccacca acatcccgtc gctggacgga
    11341 tgccacctcg gcgagcgtct gggagcactg ctcggcgtgc ccgtcgacgt ggtcaatgac
    11401 gggcacgcgg ccgccgtcgc cgaggcgacc tggggcgccg gcgcgggcag cgacgac
//
LOCUS       DATMSX010000419         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101005,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000419 DATMSX010000000
VERSION     DATMSX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101005"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VN041_13415"
     CDS             <1..915
                     /locus_tag="VN041_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWU30074.1"
                     /translation="WAPTVRGVRILLGPLAQALVRAGRRVTPGGGRSSFASEEQLLSM
                     VDEAASHDLIEEDDRDLIHSVFDFTDQFVRAVMVPRTEMLTVDAAETTTDAMELFLRT
                     GVSRMPVVDDEADDVVGVLYLKDLVQFAYRDESAWRGAPVRPIARPAVFVPESMRAET
                     LLQQMKRDAVHVCIVIDEHGGISGLVTLEDLIEELVGEISDEYDRGPAEVVELGGGRY
                     RVNSRLPLEDVGDLFGLELEDEDVDSIGGLLGKALGQIPQPGATASVDGLVLTGGASR
                     GRGRGIATVFVERAPEENEDVDGGRADD"
     gene            908..1819
                     /gene="era"
                     /locus_tag="VN041_13420"
     CDS             908..1819
                     /gene="era"
                     /locus_tag="VN041_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWU30075.1"
                     /translation="MTDSTRSGFVTFVGRPNVGKSTLTNALVGEKIAITSEKPQTTRR
                     AIRGILNRPDGQLVIVDTPGIHKPRTLLGERLNDLVDQVLGDVDVIGFCVPATEKVGP
                     GDRRIAASLDGYPRAKKVAIVTKTDAADKDDIVERLMEVDSLREDWAAVIPLSALGDG
                     SMGRDQLNVLADEMLLLMPEGPRLYEDGVVTDESDEDRIAEIIREAALEDVRDELPHS
                     IAVVVDDIEPREDSDIIDVYASIVVERDSQKAIIIGRKGARLRDVGTAARVGIEEMLG
                     SRVFLKLHVRVAKEWQRDPKQLGRLGF"
     gene            1821..>2373
                     /locus_tag="VN041_13425"
     CDS             1821..>2373
                     /locus_tag="VN041_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWU30076.1"
                     /translation="MAPQRTAVHWVAIEPGTPDAWRFEQYEVPAPAPGEVTVRVRAAG
                     VNPADAKHVASARPGVEFPVPIGYEVSGVVTAVGADTEVVTGPVSVGDEVIAFRVSGG
                     YATELTVPAQKVLRKPAALTHAEAANLLLAGSTASAMLHAVGAGPGETLLLHAASGSV
                     GASVLQQARAQGIRIIGTASPARF"
BASE COUNT          359 a          786 c          894 g          334 t
ORIGIN      
        1 tgggcgccga cggtgcgcgg cgtgcgcatc ctgctcggcc cgctggccca ggcgctcgtg
       61 cgcgccggcc gccgggtcac cccggggggc gggcgcagca gcttcgcatc cgaggagcag
      121 ctgctcagca tggtcgatga ggcggcgtcg cacgacctca tcgaggagga cgaccgcgac
      181 ctcatccact cggtgttcga cttcaccgat cagttcgtcc gagccgtcat ggtgccgcgc
      241 acggagatgc tcacggtgga cgccgccgag acgacgacgg atgcgatgga gctgttcctg
      301 cgcaccggcg tctcgcggat gcccgtggtc gacgatgagg ccgatgacgt cgtcggcgtg
      361 ctgtacctga aggatctcgt gcagttcgcc taccgggacg agagcgcctg gcgcggcgca
      421 ccggtgcggc cgatcgcgcg ccccgccgtc ttcgtgccgg agtcgatgcg cgcggagacg
      481 ctgctgcagc agatgaagcg ggatgccgtg cacgtgtgca tcgtgatcga cgagcacggc
      541 ggcatctcgg gcctggtcac cctggaggac ctgatcgagg agctggtcgg ggagatctcc
      601 gacgagtacg accgcggacc ggccgaggtg gtcgagctcg gcggcggacg ctaccgggtg
      661 aactcccggc tcccgctgga ggatgttggc gacctgttcg ggctggagct cgaggacgag
      721 gacgtggact cgatcggcgg tctgctcggc aaggcgctgg ggcagatccc gcagcccggg
      781 gcgacggcat ccgtcgacgg cctggtgctc accggcggcg cctctcgggg ccgggggcga
      841 ggcatcgcga ccgtgttcgt ggagcgtgcc ccggaggaga atgaagacgt cgatggagga
      901 cgcgcagatg actgacagca cccgaagcgg attcgtgacg ttcgtgggtc ggcccaacgt
      961 cggcaagtcc acgctgacca acgcgctggt gggggagaag atcgccatca ccagtgagaa
     1021 gccgcagacc acgaggcggg cgatccgagg catcctcaac cgtccggacg ggcagctcgt
     1081 gatcgtcgac acccccggca tccacaagcc ccgcacgctg ctcggcgagc ggctgaacga
     1141 cctggtggat caggtgctcg gcgacgtcga cgtgatcggc ttctgcgtgc cggcgacgga
     1201 gaaggtgggc cccggcgatc gccgcatcgc agcatcgctg gacggctatc cccgtgccaa
     1261 gaaggtcgcg atcgtcacca agacggatgc cgccgacaag gacgacatcg tggagcgcct
     1321 catggaggtc gactctctgc gcgaggactg ggccgcggtc atcccgctgt cggcgctcgg
     1381 cgacggctcg atgggccgcg accagctgaa cgtgctggcc gacgagatgc tgctgctgat
     1441 gcccgaaggg cctcggctgt acgaggacgg cgtcgtcacg gacgagtccg acgaggaccg
     1501 catcgccgag atcatccgcg aggccgcgct ggaggacgtg cgcgacgagc tgccgcactc
     1561 gatcgccgtg gtggtcgacg acatcgagcc tcgcgaggac agcgacatca tcgacgtgta
     1621 cgcctcgatc gtcgtcgagc gcgacagcca gaaggccatc atcatcggac gcaagggcgc
     1681 gcgtctgcgc gatgtgggga ccgccgcgcg cgtgggcatc gaggagatgc tcggctcccg
     1741 cgtgttcctc aagctccatg tgcgcgtggc caaggagtgg cagcgcgacc ccaagcagct
     1801 cgggcgcctc gggttctgac gtggcgccgc agcggacggc cgtgcactgg gtcgcgatcg
     1861 agccgggaac gccggacgcc tggcgcttcg agcagtacga ggtgccggcg cccgcacccg
     1921 gcgaggtcac ggtccgcgtg cgggcggccg gtgtgaaccc ggcggatgcc aagcacgtcg
     1981 cctccgcgcg ccccggcgtc gagttccccg tgccgatcgg gtacgaggtc tcgggcgtcg
     2041 tcaccgccgt cggcgcggac accgaggtcg tcaccggccc ggtgtcggtg ggcgacgagg
     2101 tgatcgcctt ccgggtgagc gggggctacg ccaccgagct gacggtgccg gcgcagaagg
     2161 tgctgcgcaa gcccgctgcg ctgacgcacg ccgaggcggc caacctgctg ctggcgggct
     2221 cgacggcatc cgcgatgctg cacgcggtcg gcgcaggacc gggggagacg ctgctgctgc
     2281 acgccgcctc cggctctgtc ggcgcgagcg tgctgcagca ggctcgggcg cagggcatcc
     2341 ggatcatcgg cacggcgagc cccgcccgct tcg
//
LOCUS       DATMSX010000420         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_186326,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000420 DATMSX010000000
VERSION     DATMSX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186326"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VN041_13430"
     CDS             complement(<1..833)
                     /locus_tag="VN041_13430"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HWU30077.1"
                     /translation="MTSDQPARTGVVIVGSVTADVTTFSQRLPARGETILGDQFTLLL
                     GGKGANQAVAAGLAGARTSFVGCVGDDLFHDLIVDGLSGAGVDLTHLRTVPGPTGIAH
                     IRVDASAQNDIVMVPLANAQLSAEQIDAALEALAPTTSVLLTQLETPASLTLHITQRA
                     RQLGMTVVLDPAPAATLDAEVWRSIDIVTPNETEATLISGIEVTDAASAEEAGRWFLA
                     QGVGSAVITMAEKGSCVVTAEGTSFIEPIPVEPIDTTAAGDAYAGYLGAALAEGWTLA
                     DA"
     gene            947..1603
                     /locus_tag="VN041_13435"
     CDS             947..1603
                     /locus_tag="VN041_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWU30078.1"
                     /translation="MTAPRLYVDSADTERVGRLLAAGVVHGVTTNPTILERGGRTASE
                     IPSLYARWVEEGAQEVFFQTWGGDRDAFLRNAAGILELGERVAVKVPATAEGFAAASA
                     LVRDGASVLVTAVYSVAQALACASIGVRYIAPYLGRMRDAGLDGADLITRMQAVCEGT
                     DTNVLAASLRSPQDIVELRLAGVPYFTAAPDVLDALLHDPVSDSSAEEFDAAMGRLGA
                     "
     gene            complement(1834..2514)
                     /locus_tag="VN041_13440"
     CDS             complement(1834..2514)
                     /locus_tag="VN041_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWU30079.1"
                     /translation="MAVTDEAIEKIKAMIVTGELGPGDRLPPEKELAERLGLSRNSMR
                     EAVKALEVIRVLDVRRGDGTYVTSLEPHLLLEAISFVVDMHDDSSLLELFAVRRMLES
                     QATGLAAGNASEEQAQGLLDEIGSVDADAVSIDDLVAHDIRFHGSIVRLAGNDYLASL
                     IDGLSSQTIRARVWRGLTEQGAVDRTLSEHLAIAEAIAQHDSALATSLATAHVAGVER
                     WLRQAANA"
     gene            2562..3440
                     /locus_tag="VN041_13445"
     CDS             2562..3440
                     /locus_tag="VN041_13445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU30080.1"
                     /translation="MRVIDSHLHLWDPEVLTYEWLEGPLAWRFAEEEIYEERLDGVSE
                     EAAIFVQADPIESQSLDEVRWVDSIALAAGVVAIVAGARLDRGASTDEQLDALSAFDR
                     VVGVRHLLQGEPDGFALTSSFQRGAREVARRGWTFDACVRAHQLADVAALAASVPDLP
                     IVLDHVGKPAVGSASSPVGPSPDWLGDLGALAQHPQVFCKLSGLPAESGGVWTDAQMQ
                     PFLDAALDAFGPERLMWGSDWPVSSIDAGRLDGGAYVVGSRQRWFRTVADWAAQRGLD
                     VEALFWSNALTFYGIR"
     gene            complement(3664..4677)
                     /locus_tag="VN041_13450"
     CDS             complement(3664..4677)
                     /locus_tag="VN041_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30081.1"
                     /translation="MPAPRPLPESLGRRFSVASAVEAGVGRGRLRRADLEAPFHSVRA
                     RRIETALDDLDPYTKQVTERRIQADNYAPRLRADHCLSHESAVAIWGGPLPLVLPPLT
                     DPEWRTPGADRRPRQPVSGLLLDVDVSTLGDGPLVRARGVRGHRADPAVVSLRKVRGF
                     LVASPATAWAQLGHLSLLDLVALGDHFCRRWRDGHGRSNAGRRPLATVDELREKIAST
                     RWRGIRRLREAVDLVREDSWSPRESHVRCHLVLSGLPEPVLNHDAYDRHGAFLGCVDL
                     AYPERRVAIEYQGLLHSAQYAADVERIAALRAAGWTVIEVTSELLARPSLLIARVRAA
                     LQS"
     gene            complement(4961..>5100)
                     /locus_tag="VN041_13455"
     CDS             complement(4961..>5100)
                     /locus_tag="VN041_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30082.1"
                     /translation="PHGRLVSPDEVAGAVLYLVSPASGSTTGTFIEVDGGMAALRLRA
                     E"
BASE COUNT          727 a         1708 c         1868 g          797 t
ORIGIN      
        1 gcatcggcga gcgtccagcc ctcggcgagg gcggcgccca ggtacccggc gtacgcgtcg
       61 ccggcggcgg tcgtgtcgat cggctcgacg gggatgggct cgatgaacga ggtgccctcg
      121 gcggtcacca cgcaggagcc cttctccgcc atggtgatca cggccgagcc cactccctgc
      181 gccaggaacc agcgaccggc ctcctcggcc gaggcggcgt cggtgacctc gatcccgctg
      241 atgagcgtgg cctcggtctc gttgggggtg acgatgtcga tgctgcgcca cacctcggcg
      301 tccagcgtcg ccgcgggtgc ggggtcgagc accacggtca tgcccagctg gcgtgcgcgc
      361 tgggtgatgt gcagcgtgag ggatgccggc gtctccagct gcgtgagcag cacggaggtg
      421 gtcggggcga gcgcctcgag tgcggcgtcg atctgctcgg cgctgagctg agcgttcgcc
      481 aggggaacca tcacgatgtc gttctgcgcc gaggcgtcga cgcggatgtg cgcgatgccg
      541 gtgggcccgg ggaccgtgcg caggtgggtg aggtcgacgc ccgcgccgct gagcccgtcg
      601 acgatgaggt cgtggaacag gtcgtccccc acgcatccga cgaagctcgt gcgggctccg
      661 gccaggcccg cggcgacggc ctggttcgca cccttgcctc cgagcagcag cgtgaactgg
      721 tcgccgagga tggtctcgcc gcgagcgggc agacgctggg agaaggtggt cacgtcagcg
      781 gtcacgcttc cgacgatgac gacgccggtg cgggcgggct ggtcgctggt catgttctcc
      841 cgatcgtctt tgacttcaga tggacgctgt cattacatta tcggcagagt agcctgtagt
      901 gacaggttct cgggctgccc gtcaccgggc caggagagga gaccggatga ccgcgccccg
      961 gctgtacgtg gacagcgctg acaccgagcg ggtcggtcga ttgctcgccg cgggtgtcgt
     1021 gcacggcgtc acgacgaacc cgacgatcct cgagcgcggc ggacgcacgg catccgagat
     1081 cccgtcgctg tacgcacgat gggtcgagga gggcgcgcag gaggtgttct tccagacctg
     1141 gggcggagac cgggacgcgt tcctgcgcaa cgccgccggc atcctcgaac tcggggagcg
     1201 ggtcgccgtg aaggtccccg ccacggctga gggcttcgca gcagcatccg ccctggtccg
     1261 cgacggagcc tcggtgctgg tgacggccgt ctactccgtg gcgcaggcgc tcgcgtgcgc
     1321 gagcatcggg gtgcggtaca tcgcgcccta tctcgggcgg atgcgcgacg ccggcctcga
     1381 cggcgccgat ctcatcaccc ggatgcaggc cgtgtgcgag ggcaccgaca cgaacgtgct
     1441 ggccgccagc ctgcgctcac cgcaggacat cgtcgagctg cggctcgcgg gcgtgccgta
     1501 cttcacggcc gcgcccgacg tgctcgacgc gctgctgcac gatcccgtga gcgactcgtc
     1561 ggccgaggag ttcgacgccg cgatggggcg cctgggcgcc tgacgcagag aaccctccgc
     1621 caggtcgcag ttcgtcccgg aatgccgcac tcttgtccgt ccagatcgca gttcgtcccg
     1681 gaatgccgca ctcctgtccg tccagatcgc agttcgtccc gtaatccgac gcggaaagcg
     1741 ggacgaatcg cgacctggag cccgggcgtc aggcaatcgc gactggagcc cgggcgccaa
     1801 gcattggcgg cccggatgtc aggcgtccgg gcgtcaggcg ttcgcggcct gacgcagcca
     1861 gcgctccacg ccggcgacgt gggcggtggc cagcgaggtc gccagcgcag agtcgtgctg
     1921 ggcgatcgcc tcggcgatgg ccagatgctc ggacagcgtg cggtccaccg ccccctgctc
     1981 cgtcagcccg cgccacacgc gggcgcggat cgtctggctg ctgagcccgt cgatcaggct
     2041 cgccaggtag tcgttgccgg ccagtcgcac gatggagccg tggaagcgga tgtcgtgggc
     2101 gacgaggtcg tcgatgctga cggcatccgc gtcgacggag ccgatctcgt cgaggagtcc
     2161 ctgcgcctgc tcttcgctgg cgttgcctgc ggcgagaccg gtggcctgcg attccagcat
     2221 ccgccgcacc gcgaacagct cgagcagcga gctgtcgtcg tgcatgtcga cgacgaagct
     2281 gatcgcctcc agcagcagat gcggctcgag gctggtgacg taggtgccgt ccccgcggcg
     2341 cacatcgagc acccggatga cctcgagcgc cttgacggct tcgcgcatgg agttgcgcga
     2401 caggccgagc cgctccgcga gctccttctc cggcgggagc cggtcgccgg ggccgagctc
     2461 gcccgtgacg atcatcgcct tgatcttctc gatcgcctca tccgtgactg ccatggccac
     2521 atcttcgcac agccatccga tgtttccggc aggatggtgc catgcgcgtc atcgattccc
     2581 acctgcacct gtgggacccg gaggtcctca cctacgagtg gctcgagggc ccactggcct
     2641 ggcgcttcgc cgaggaggag atctacgagg agcggctcga cggcgtctcg gaggaggcgg
     2701 cgatcttcgt gcaggccgac ccgatcgagt cgcagtccct cgacgaggtg cgctgggtgg
     2761 actcgatcgc gctcgccgcg ggagtcgtgg cgatcgtcgc cggggcgcgg ctggatcgtg
     2821 gtgcgtcgac ggatgagcag ctcgacgcgc tgagcgcttt cgaccgcgtc gtcggtgtgc
     2881 ggcatctcct gcagggcgaa ccggacggct tcgcgctgac gtcgtcgttc caacgcggtg
     2941 cgcgggaggt cgcacgccgc ggctggacct tcgacgcctg tgtgcgggcg caccagcttg
     3001 cggatgtggc ggcgctcgcg gcatccgtcc cggatctgcc catcgtgctc gaccacgtcg
     3061 gcaagcccgc ggtgggttcg gcgtcgtctc cggtcgggcc ttccccggac tggctgggcg
     3121 atctgggtgc cctggcgcag catccgcagg tgttctgcaa gctgtccgga ctgcccgccg
     3181 aatccggcgg ggtgtggacg gatgcccaga tgcagccgtt cctggatgcg gcgctcgacg
     3241 cgttcgggcc ggagcggctg atgtggggga gcgactggcc ggtgtcgtcg atcgacgccg
     3301 gtcgcctgga tggcggggca tacgtggtgg gctcccgtca gcgctggttc cgcacggtcg
     3361 ccgactgggc ggcacagcgt ggcctggacg tcgaggcgct gttctggtcg aacgcgctga
     3421 ccttctacgg catccgctga cactcgcgcg gaccggccgc tcacgcgcgg ccggatgtcg
     3481 gcactcgcgc ccgcaggtcc gctcacgcgc ccccggtcgc attggatgcc gtctctgggc
     3541 cgctgatctc gctccaggtc gcaatggatg ccgctgtgat atcgaagcgg cggcatccat
     3601 cgcgacctgg acccagaagc ggcagacacg aacggcattc accgcgacct ggattcgctg
     3661 gggtcagctc tgcagtgctg ctcgtacgcg ggcgatgagc agggacggtc tggcgagcag
     3721 ttcgctggtg acctcgatca cggtccagcc ggccgctcgg agtgcggcga tgcgctcgac
     3781 gtctgcggcg tactgcgcgc tgtgaagcag accctgatac tcgatggcga ccctcctctc
     3841 gggatatgcg agatcgacgc agccgaggaa cgcgccgtgc cggtcgtacg cgtcatgatt
     3901 gagcacgggc tccggaaggc cggacagcac caggtgacag cgcacgtgcg actccctggg
     3961 agaccacgag tcctcgcgga cgagatcgac tgcctcgcgc aggcgccgga tgccccgcca
     4021 tcgggtggac gcgatcttct cgcgcagctc atcgaccgtc gccagaggcc ttcggcccgc
     4081 gttcgagcgg ccatggccgt ctcgccacct tcggcagaag tgatcgccca gagcgacgag
     4141 atcgagaagg ctcagatgtc cgagctgggc ccacgccgtc gcggggcttg ctacgaggaa
     4201 ccctcgcacc ttgcgcagag agacaaccgc cgggtcggcc cgatggccgc gcacaccccg
     4261 tgcgcgaacg agaggtccgt cgccgagcgt gctgacgtcg acgtccagga gaagcccgct
     4321 cacgggctga cggggtcggc gatcggcgcc cggcgtgcgc cattcgggat ccgtcagcgg
     4381 agggagcacg agcggcaggg gcccgcccca gatggcgacg gcggattcat ggctgagaca
     4441 gtgatcggcc cggagtctcg gcgcatagtt gtcggcctgg atccgacgtt cggtgacctg
     4501 cttcgtgtaa gggtccaggt cgtcgagagc ggtctctatc cgccgcgcac gcacgctgtg
     4561 aaacggtgcc tcgagatcag cgcgccgcag gcgtcctcgg ccgacgcccg cctcgacggc
     4621 ggatgcgacg ctgaatcgtc gtccgaggga ttcggggagc gggcgtggcg cgggcatcct
     4681 cccaccctgc cgagcacgcg cgggcgccgt ggaagttatc cacagggagt gacagcatcc
     4741 aggtcgcagt ggatgccgtc gtgcggtgtg ttgccggcgt ccaggtcgca gtggatgccg
     4801 ctgacccgcg caagaggcgg catccaatgc gacctgggct tgggaggccc cgacccgacg
     4861 gcatccagtg cgacctggac aacagatggc agctgggcgc ccgcgggaaa cgcgaggggc
     4921 cgccacgcgg atgcgggacg gccctccgaa cgggaacgga ctactcggcg cggaggcgca
     4981 gggcggccat gccgccgtcg acctcgatga acgtgccggt cgtcgacccg gaggccgggc
     5041 tgaccaggta gagcacggcg ccggcgacct cgtcggggga gaccaggcgg ccgtgcggct
//
LOCUS       DATMSX010000421         8844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_6737,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000421 DATMSX010000000
VERSION     DATMSX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6737"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VN041_13460"
     CDS             complement(<1..203)
                     /locus_tag="VN041_13460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU30083.1"
                     /translation="MPSFLDLGVPAALANVLAADGKTEPFAIQRDTLPDSLAGRDVLG
                     RGRTGSGKTIADLRCFAVEAGAVV"
     gene            complement(497..1450)
                     /gene="dapD"
                     /locus_tag="VN041_13465"
     CDS             complement(497..1450)
                     /gene="dapD"
                     /locus_tag="VN041_13465"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWU30084.1"
                     /translation="MSTARTVWGTGLSTIASDGTVLDAWFPELGFGEQDAEDAAAAAF
                     TWDAFAEPDERRGVTVEFVQLSIDLDAPVASTPDAYLRLHALSHLLVRPNEVSLDGIF
                     AHLPTVAWTNAGPMLPADAARLRPLLQRHGIQVQGLDKFPRLTDYVQPAGVRIADASR
                     VRLGAHLSPGTTVMHEGFVNFNAGTLGASMVEGRISQGVVVGDGSDIGGGASIMGTLS
                     GGGTHRISVGARTLLGANAGIGISLGDDCIVEAGLYLTAGTKVELADAAPTAEGRRQT
                     VKAAALSGEDGLLFRRNSLSGAVEVVRRAGAGVTLNEALHA"
     gene            1518..2591
                     /gene="dapE"
                     /locus_tag="VN041_13470"
     CDS             1518..2591
                     /gene="dapE"
                     /locus_tag="VN041_13470"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWU30085.1"
                     /translation="MVLDLTASSADITRVICDIPSVSGDEARLADEIEQTMRAFPHLE
                     VTRHGNTIVARTDLGRAQRVVIAGHIDTVPINGNVPTRDIEIDGVPHLWGRGTVDMKA
                     GVAVQLKLAAELTEPAADITWMWYDNEEVQESLNGLFLLASDRPDLFQADFAILGEPS
                     VGRIEGGCNGTMRAVIRTRGVRAHSARSWVGENAIHGAAPILTRLAEYRACEIMVEGL
                     GYREGLNAVSIRGGVAGNVIPDLCEVEVNYRFAPSKSVADAEAHIRAAFDGFEVEITD
                     AAAGARPGLDAPIAQRFLAAVGGEAHAKYGWTDVARFSALGVPAVNYGPGDPHLAHHD
                     EERVPLAQIEAVERGLRAWLTAP"
     gene            2573..3787
                     /locus_tag="VN041_13475"
     CDS             2573..3787
                     /locus_tag="VN041_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30086.1"
                     /translation="MADGTLSSALRRWARVPVVGRIALVYLTARLVTTGFLVLASTVS
                     TSQSRFGPAPSLRSFVLGWDAQWYWWVAVNGYPQVLPFSATGQVAENSWAFMPVYAYV
                     ANAVGLGDWGLGALLVSLVSGLLACIVLHRLLRPRIGASAAMWAVAFFASGPLAAMFQ
                     VGYAESLFTLLLLLGLDLVARRRYAWLYLVLPVMGFTRPGILAFALFLGLHGILRWVR
                     RDCEPLPVRHVVHIVALGLLSVVVGFAWQVIAGVVTGDPSAYLKTELAWRRNWLPESN
                     DSFFPFEGFVRGAEFWAGQLGVPAGGGVIALVVLVVAVAALLLRSASARRLGPEIRLW
                     SASYLLYLLAVFFPQSSTLRLLMPLTPLWGALAVPRSRTYRWGMLGLCLLAQWLWIVS
                     MYGIASTFYQVP"
     gene            3902..4075
                     /locus_tag="VN041_13480"
     CDS             3902..4075
                     /locus_tag="VN041_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HWU30087.1"
                     /translation="MAAMKPRTGDGPMEAVKEGRLIIVRVPLEGGGRLVVSVNDAEAK
                     ELHDVLGAVVTAA"
     gene            complement(4157..4786)
                     /locus_tag="VN041_13485"
     CDS             complement(4157..4786)
                     /locus_tag="VN041_13485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30088.1"
                     /translation="MSEHDANARFARETIVEPAPISRARTHALELGAAPISPGIGAQY
                     AVLAASVAARSIVEIGTGAGVSGLWLLRGAPQAVLTTIDNEPEHLAAARQAFAEAKIP
                     PAKARFITGRAADVLPRMNEGSYDIVLIDADPENVIEYVEHGLRLVRPGGLVLVPRVL
                     TGGKVADPVQRDEVTSAYRSLVQETQESSAVLAALSTTGEGLLQLSRIA"
     gene            4873..5265
                     /locus_tag="VN041_13490"
     CDS             4873..5265
                     /locus_tag="VN041_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWU30089.1"
                     /translation="MNWGLDMDKLLLIGIVAVLIIGPERLPKAAEALRRFARQAAAYL
                     RDTKDKMKDELGEDIDDVDWRKLDPRQYDPRRIIRDALFEEPAFDSAPATTSNPFTTD
                     PVAAPERAPMTRQVFSRMSPPPFDVEAT"
     gene            complement(5304..6443)
                     /locus_tag="VN041_13495"
     CDS             complement(5304..6443)
                     /locus_tag="VN041_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWU30090.1"
                     /translation="MTLAEQVRAAVSAVTDPELRRPIGDLDMVRDVAVDGNAATVSIV
                     LTIVGCPAARRIEEDVRDAAASVSGIAEVTVDVGVMTPDERRALTERLRGGRAARQMP
                     FGPDSLTRVILVSSGKGGVGKSTVTANLAVALARQGLRVGLVDADVHGFSIPGLLGIA
                     PGTQPTRIDDLMLPPVAHDVKTVSIGMFLREGESVVAWRGPMLHRTVQQFLTDVFFGD
                     LDVLLIDMPPGTGDIAISIGQILPHAEVLVVTTPQPAAAEVAIRSGLVARRTGQRVIG
                     VVENMAPYALPDGTVIDLFGSGGGAEVAKALSAPGDHGSVAEDVPLLASIPLSPALRQ
                     GGDAGMPVVLEQPEDAAGAAIISLAHTIAHRGRGLAGRALPMRLS"
     gene            complement(6440..6961)
                     /locus_tag="VN041_13500"
     CDS             complement(6440..6961)
                     /locus_tag="VN041_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HWU30091.1"
                     /translation="MARTSSLDTPRGRAGAARPSAPSRDRFGRFTEWVARAMGTPAFL
                     VILTLFCAAWIVWNIAMPEPLRFDDAALGFTALTLMLSLQASYAAPLILLAQNRQDDR
                     DRVQIEQDRQRAERNLADTEYLAREIVALRLALEERNPQIVTRDVLRQELKSLLAELD
                     RDGTKDTTAESAP"
     gene            complement(6961..8223)
                     /locus_tag="VN041_13505"
     CDS             complement(6961..8223)
                     /locus_tag="VN041_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWU30092.1"
                     /translation="MSTQRVFAARLAGCAVFDPVGDRLGKVRDVVIVYRKTAAPRVIG
                     LVVEIPGRRHVFLSIGRVTSIRAGQVISTGLINVRRFQPRPGEVRVLAEFLGRRVSLA
                     DGSGTAVIEDAAIEPDRHGEWAITQLFLRKPKTSASPFAKGPTTFASWNEVTEQQHPG
                     EAQSAEQLVATYSELHAADLATTLLDLPEQRLIEVAEELPDERLADALEEMPEDEQVG
                     ILDRLGDERAADVLDEMEPDDAADLLAQMPPERLEQLLALMEPEEAEDVRTLLRYGPD
                     TAGGLMTTEPIILSADATVAEALALIRRHELHPALAAAVFVTLPPFETPTGRLLGVVH
                     FQRMLRYPPHERLGAILDDNIEPVSVTASAAEVARMLASYDLVSLPVIDSAHRLVGAI
                     SVDDVLDYLLPNDWRTHDEDEPEASGTR"
     gene            8301..>8844
                     /locus_tag="VN041_13510"
     CDS             8301..>8844
                     /locus_tag="VN041_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HWU30093.1"
                     /translation="MSMMNRPGNGTDLGEMVASSRDYEVAQKTVSKLIAGEVPARDIA
                     IVGQGVRTVERVTGRLGYAAAARSGAINGVLIGLVLSAVMLFGNSNAPVSLFLGFILI
                     GVALGMVMSLVTYSIIRRRRDFASMTQMLADHYEVRVQAASLAKARQVLGSERQETPR
                     PTVDLSKPPQFGERLPAPGAD"
BASE COUNT         1260 a         2991 c         3219 g         1374 t
ORIGIN      
        1 acaacagcgc cggcttctac cgcgaagcag cgtaaatccg cgatggtctt gccgctgccg
       61 gtgcggccgc ggccgagcac gtcacgcccg gccagggagt cggggagggt gtcgcgctgg
      121 atggcgaagg gctcggtctt gccgtcggcc gcgagcacgt tcgcgagggc ggcgggcacg
      181 ccgaggtcga ggaaggaagg catgggtgtc tccataagga aggcgcaggg atgctgcgga
      241 atgggcgacg gcgcgggttc gcgcatcggt tcgccgctcg agatgtgacc gcggaccgga
      301 tgccggggcg gaagtgtgtc gacgacgcag acgtctcgct tggcgagagg cgtcggatca
      361 accctagcag tccctgatgg tcgcggctgt gcatacgccg acacccccat tctccgccga
      421 gacccctgcc tgcggacgtc cgcaggcagg ggtctggacg gcagatgggg gtgtcgacgg
      481 ccgaagggat accgggtcag gcgtgcaggg cttcgttcag cgtgacgccg gcgcccgctc
      541 gccggacgac ctcgacggcg ccgctcaggg agttgcggcg gaacagcaga ccgtcctcgc
      601 cggagagtgc ggcggccttc acggtctgcc ggcgaccctc ggcggtcggt gccgcatccg
      661 cgagctcgac cttggtgccc gccgtgaggt agagccctgc ttccacgatg cagtcgtcgc
      721 ccagggagat gccgattccg gcgttcgcgc cgagcagggt gcgggctccc accgagatgc
      781 ggtgcgtccc tccaccggac agcgtgccca tgatcgaggc gccgccgccg atgtcgctgc
      841 cgtctcctac gaccacgccc tgggagatgc gtccctcgac catcgaggcg cccagggtgc
      901 cggcgttgaa gttgacgaag ccttcgtgca tcacggtcgt cccgggggag aggtgcgcgc
      961 ccagccgcac gcgcgaggcg tcggcgatgc gcacacccgc aggctgcacg tagtcggtga
     1021 gccgggggaa cttgtccagt ccctgcacct ggatgccatg gcgctgcagc agcggccgga
     1081 gccgcgctgc gtcggcgggg agcatggggc ccgcgttggt ccaggccacg gtgggaaggt
     1141 gcgcgaagat gccatccagg ctgacctcgt tcggtcgcac gagcaggtgc gacagcgcgt
     1201 gcagacgcag gtaggcgtca ggggtggacg cgacgggcgc gtccaggtcg atgctgagct
     1261 gcacgaattc gaccgtcaca ccgcgacgct cgtcgggttc ggcgaatgcg tcccaggtga
     1321 aggcggccgc ggcggcgtcc tcggcatcct gctcgccgaa gccgagttcg gggaaccatg
     1381 cgtccaggac ggtgccgtcg gaggcgatcg tgctgaggcc cgtgccccag accgtgcgtg
     1441 ctgtgctcat cctgcaacgc tagcgcgaca ggtcatgcgg aggctgggca tgtgacgcac
     1501 ggtttagtgt gggtgacatg gtgctcgatc tgaccgcatc ctctgctgac atcacccgcg
     1561 tgatctgcga catccccagc gtctccggcg acgaggcccg cctcgccgac gagatcgagc
     1621 agaccatgcg ggccttcccg cacctggagg tgacgcggca cggcaacacg atcgtcgcgc
     1681 gcaccgacct gggccgcgcg cagcgcgtcg tgatcgcggg ccacatcgac acggtcccca
     1741 tcaacggcaa cgtgcccacg cgcgacatcg agatcgacgg cgtcccgcac ctgtgggggc
     1801 gcggcacggt cgacatgaag gccggcgtgg cggtgcagct caagctcgct gccgagctca
     1861 ccgaacccgc ggccgacatc acctggatgt ggtatgacaa cgaagaggtc caggagtcgc
     1921 tcaacggcct gttcctgctg gcatccgatc gccccgacct gttccaggcc gacttcgcga
     1981 tcctcgggga gccgtccgtc ggacgcatcg agggcggatg caacggcacg atgcgcgccg
     2041 tgatccgcac ccgcggagtg cgggcgcaca gcgcgcgctc ctgggtgggc gagaacgcca
     2101 tccacggcgc cgccccgatc ctcacacgtc tggccgagta ccgtgcctgc gagatcatgg
     2161 tcgaggggct cggctaccgc gagggactga acgccgtcag catccgcggc ggggtggcgg
     2221 gcaacgtcat ccccgacctg tgcgaggtcg aggtcaacta ccgcttcgcg ccgagcaaga
     2281 gcgtggcgga tgccgaggcg cacatccgtg cggcgttcga cggcttcgag gtcgagatca
     2341 cggatgccgc ggccggcgcc cggcccggcc tcgacgcacc catcgcgcag cgcttcctgg
     2401 ccgccgtcgg cggcgaggcg cacgcgaagt acggctggac cgatgtggcg cggttctccg
     2461 cgctcggcgt ccccgccgtc aactacggcc ccggcgaccc gcatctcgcc caccacgacg
     2521 aggagcgggt gccgctcgcg cagatcgagg cggtcgaacg cgggctgcgg gcgtggctga
     2581 cggcaccctg agctccgcgc tgcggcgctg ggcgcgcgtc ccggtcgtcg ggcgcatcgc
     2641 gctcgtctac ctgaccgcca ggctcgtcac gacggggttc ctcgtcctgg cgtccacggt
     2701 gtcgacgtcc cagtcgcgct tcggaccggc gccgagcctg cgctcgttcg tgctgggctg
     2761 ggacgcccag tggtactggt gggtggccgt caacggctac ccacaggttc tgcctttctc
     2821 ggcgaccggg caggtcgccg agaactcctg ggcgttcatg ccggtgtacg catacgtcgc
     2881 gaacgcggtc ggcctcgggg actggggtct cggtgctctg ctcgtgtcgc tcgtctcggg
     2941 gctcctcgcg tgcatcgtgc tgcaccgcct gctgcgcccg cgcatcggag cctcggctgc
     3001 gatgtgggcg gtcgcgttct tcgccagcgg accgctcgcc gcgatgttcc aggtgggcta
     3061 cgccgagtcg ctcttcaccc tgctgctgct gctcgggctc gacctcgtcg cacggcggcg
     3121 gtacgcctgg ctgtacctgg tgctccccgt catggggttc acccgtcccg gcatcctggc
     3181 gttcgcgttg ttcctcggac tgcacggcat cctgcgctgg gtgcgccgtg actgcgaacc
     3241 tcttccggtg cggcacgtcg tgcacatcgt ggcgctcgga ctgctctccg tggtggtcgg
     3301 cttcgcctgg caggtgatcg cgggcgtcgt cacgggtgac ccgtccgcct atctgaagac
     3361 cgagctggcc tggcggcgca actggctccc cgaatcgaac gacagcttct tccccttcga
     3421 ggggttcgtg cgcggagcgg agttctgggc tgggcagctc ggcgttcccg ccggcggtgg
     3481 cgtgatcgcg ctcgtcgtgc tggtcgtggc ggtggcggcg ctgctgctgc gctccgcctc
     3541 ggcgcgcagg ctcggccccg agatccgcct gtggagcgcg agctacctgc tctacctgct
     3601 cgcggtgttc ttcccgcagt ccagcactct acggctgctg atgccactga ccccgctgtg
     3661 gggtgcgctg gcggtgccgc gctcgcgcac gtaccgatgg gggatgctcg ggctgtgcct
     3721 gctggcgcag tggctgtgga tcgtgtcgat gtacgggatc gccagcacgt tctaccaggt
     3781 tccatagcgg gatcagagcg gccgcacagg ccgtctgacc ggcgattgtg tcgggctgac
     3841 atgctcaggc cggatgctga cgataaactt gacgcatacc accgagggaa agggagccac
     3901 gatggcagcg atgaagccga ggaccggcga cggaccgatg gaggccgtga aggaggggcg
     3961 tctcatcatc gtgcgcgttc cgctcgaagg aggcggacgt ctggtcgtct ccgtgaacga
     4021 tgccgaggcg aaggagcttc acgacgtgct gggtgccgtc gtgactgcag cctgatcggc
     4081 gaggacgcga gaggggcgat gggtttccac ccatcgcccc tcttctcgtc tgcgccttca
     4141 gctggtgtgg gcgccgtcag gcgatccgcg acagctgcag caggccctcg ccggtcgtgg
     4201 agagggctgc gagcacggcg gaggactcct gagtctcctg caccagcgag cggtacgcgg
     4261 aggtgacctc gtcgcgctgc acggggtcgg cgaccttgcc cccggtcagc acccgcggga
     4321 ccagcaccag accgccgggg cgcaccaggc gcaggccgtg ctcgacgtac tcgatcacgt
     4381 tctccgggtc ggcatcgatc agcacgatgt cgtatgagcc ctcgttcatg cgcgggagca
     4441 cgtccgccgc gcggccggtg atgaaccgcg ccttggcggg cgggatcttc gcctcggcga
     4501 acgcctgacg tgcggcggcg aggtgctcgg gctcgttgtc gatcgtggtg agcacggcct
     4561 ggggcgcacc gcgcagcagc cagagtccgg acacaccggc accggtgccg atctcgacga
     4621 tcgatcgcgc ggcgacggat gccgccagca cggcgtactg ggccccgatt ccggggctga
     4681 tgggggcggc gcccagctcg agcgcgtggg tgcgtgcgcg ggagatgggc gcgggctcca
     4741 cgatcgtctc gcgggcgaat cgggcgttcg cgtcgtgctc gctcatggca attctcctga
     4801 aacttcggtc accgaccagc gtaggcgtct cgggtgcatc cggaggggag gcgcggcggg
     4861 tagcctggtg ggatgaactg gggcctggac atggacaagc tgctgctgat cggcattgtc
     4921 gctgtgctca tcatcggtcc cgagcgtctg ccgaaggccg cggaggcgct caggaggttc
     4981 gcccgtcagg cggccgcgta cctgcgcgac accaaagaca agatgaagga cgagctcggc
     5041 gaggacatcg acgacgtcga ctggcgcaag ctcgatcccc gccagtacga ccctcgtcgc
     5101 atcatccgcg atgcgctgtt cgaggagccc gccttcgaca gcgcccccgc gacgacgtcc
     5161 aacccgttca cgaccgaccc ggtcgctgcc ccggagcgtg ctccgatgac ccgtcaggtc
     5221 ttcagccgga tgagcccgcc gccgttcgac gtcgaggcga cctgacagtt cggggatccg
     5281 ccccggccgt atgagcgatc gcgtcagctc aggcgcatcg gcagcgcgcg acccgcgaga
     5341 ccccgtccgc ggtgcgcgat cgtgtgcgcc agcgagatga tggcggcacc ggcggcgtct
     5401 tctggctgct ccagcaccac cggcatcccg gcgtccccgc cctgccgcag cgcaggggag
     5461 agcgggatgg atgccagcag cggcacgtcc tcggcgacag aaccgtgatc accgggggca
     5521 ctgagcgcct tcgcgacctc cgcgcccccg cccgagccga acagatcgat cacagtgccg
     5581 tcgggcagcg cgtacggcgc catgttctcg accacaccga tcacgcgctg acctgtgcgg
     5641 cgcgcgacca gcccgcttcg gatggcgacc tcggccgcag ccggctgcgg tgtcgtcacc
     5701 accagcacct ccgcatgcgg caggatctgg ccgatcgaga tggcgatgtc gccggtgccg
     5761 ggaggcatgt cgatcaacag cacgtccagg tcgccgaaga acacgtcggt gaggaactgc
     5821 tgcacggtgc ggtggagcat ggggccgcgc cacgcgacga cggactcgcc ctcgcgcagg
     5881 aacatgccga tggagacggt cttcacgtcg tgcgcgaccg gtggcagcat caggtcgtcg
     5941 atgcgcgtgg gctgggtgcc gggggcgatc ccgagcagcc cggggatcga gaagccgtgc
     6001 acatcggcat ccaccaggcc gacccgcagt ccctgccggg ccagcgcgac cgcgaggttc
     6061 gccgtgacgg tcgacttccc gacaccgccc ttgccactgg acacgaggat cacgcgggtg
     6121 agcgagtcgg ggccgaacgg catctgccga gccgcgcgac ccccgcgcag tcgctcggtg
     6181 agggcgcgcc gctcgtccgg cgtcatcacg cccacgtcga cggtcacctc ggcgataccg
     6241 gagacggatg ccgcggcatc ccggacatcc tcctcgatgc gccgcgctgc ggggcatccg
     6301 acgatggtga gcacgatcga caccgtcgcg gcgtttccgt cgacggcgac gtcccggacc
     6361 atgtccaggt cgccgatggg gcggcgcagc tcggggtcgg tgaccgccga taccgccgcc
     6421 cgtacctgct cggccagcgt cacggcgcgc tctcggccgt ggtgtccttc gtgccgtccc
     6481 tgtcgagttc cgccagcagg gacttgagct cctggcgcag cacgtcccgg gtgacgatct
     6541 gcgggttgcg ctcctcgagc gccagccgca gcgcgacgat ctctcgcgcg aggtactcgg
     6601 tgtcggcgag gttgcgctcg gcccgctgcc tgtcctgctc gatctgcacg cggtcgcggt
     6661 cgtcctgacg gttctgcgcc agcaggatga ggggagcggc gtacgacgcc tgcagcgaca
     6721 gcatgagggt cagcgcggtg aagcccagcg cggcgtcatc gaatcgcagc ggctccggca
     6781 tggcgatgtt ccagacgatc cacgccgcgc agaacagcgt gaggatcacc aggaaggccg
     6841 gggtgcccat ggcgcgggcg acccactccg tgaaccggcc gaagcggtcg cgggagggtg
     6901 cggagggacg ggctgcgccg gcgcgtccac gaggtgtgtc gaggctggag gtgcgggcca
     6961 tcatcgcgtg ccgctcgctt ccggctcgtc ctcgtcgtgc gttcgccagt cgttcggcag
     7021 caggtagtcg agcacgtcgt cgacgctgat cgcgccgacc aggcggtgcg cggagtcgat
     7081 gacgggcagc gagaccaggt cgtagctggc cagcatgcgc gcgacctcgg cggcggatgc
     7141 cgtgaccgag acgggctcga tgttgtcgtc caggatggcg ccgaggcgct cgtgcggtgg
     7201 gtagcgcagc atccgctgga agtggacgac gcccagcaga cggccggtcg gggtctcgaa
     7261 cgggggcagc gtgacgaaca ccgcggcggc gagggccggg tgcagctcat gccggcggat
     7321 cagtgcgaga gcctcggcga cggttgcgtc ggcggagagg atgatcggct cggtggtcat
     7381 caggccgccg gccgtgtcgg ggccgtagcg cagcagggtg cgcacgtcct cggcctcctc
     7441 cggctccatc agcgcgagca gctgctccag gcgctcagga ggcatctggg cgagcaggtc
     7501 ggccgcgtcg tcgggctcca tctcgtcgag cacgtcggcc gcgcgctcgt cgcccaggcg
     7561 gtcgaggatg cccacctgct cgtcctcggg catctcctcg agggcgtcgg cgaggcgctc
     7621 gtcggggagc tcctcggcga cctcgatcag gcgctgctcc ggcaggtcga gcagcgtcgt
     7681 cgcgaggtcc gccgcgtgca gctcggagta cgtggcgacc agctgctcgg cggactgggc
     7741 ctcaccgggg tgctgctgtt cggtgacctc gttccacgag gcgaacgtcg tcgggccctt
     7801 cgcgaacggc gacgcgctgg tcttcggctt gcgcaggaac agctgcgtga tggcccactc
     7861 accgtgccgg tccggctcga tcgcggcgtc ctcgatcacg gcggtgccac tgccgtcggc
     7921 gaggctgacc cgacggccca ggaactcggc gagcacgcgc acctcgccgg gacgcggctg
     7981 gaagcggcgc acgttgatga gcccggtgct gatcacctgt ccggcacgga tggaggtgac
     8041 gcgcccgatg gacaggaaca catggcgacg cccggggatc tcgacgacga gtccgatcac
     8101 gcgcggtgca gccgtttttc ggtacacgat gacgacgtcc cggaccttgc cgagccggtc
     8161 gcccacggga tcgaagacgg cgcaacctgc caggcgcgcg gcgaaaaccc gttgtgtgct
     8221 cacgggttcc agcgtagtcg gcgcatcgcc gggagcgccg tcggatgacc tctcaccggg
     8281 cagtctggaa caatgggacg atgagcatga tgaatcggcc cgggaacggc accgacctcg
     8341 gcgagatggt cgcgtcgtca cgcgactatg aggtcgcgca gaagaccgtc tcgaagctga
     8401 tcgccggcga ggtgcccgcc cgcgacatcg cgatcgtggg gcagggagtg cgcaccgtcg
     8461 agcgcgtgac aggcaggctc ggctatgcgg ccgcagcccg ctcgggcgcg atcaacggcg
     8521 tgctgatcgg cctcgtgctc tcggcggtca tgctcttcgg caactcgaac gctcccgtct
     8581 ctctcttcct cgggttcatc ctgatcggcg tcgctctggg catggtgatg agcctggtga
     8641 cctactcgat catccgccgt cggcgggatt tcgcgagcat gacgcagatg ctcgccgacc
     8701 actacgaggt gcgcgtgcag gcggcgtcgc tcgcgaaggc acgtcaggtg ctgggctcag
     8761 agcgtcagga gaccccgcgc ccgaccgtcg acctgagcaa gccgccgcag ttcggcgagc
     8821 ggctgccggc gccgggtgcg gatg
//
LOCUS       DATMSX010000422         5283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101045,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000422 DATMSX010000000
VERSION     DATMSX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101045"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VN041_13515"
     CDS             complement(<1..304)
                     /locus_tag="VN041_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU30094.1"
                     /translation="MMLSELVATTDEVAATSSRLAKIDALARLLERADPDEVVPLVGL
                     LLASPRQGRLGVGWRTLTSLDVTHTTAAVLTITDVDRMLDALAAASGSGSAAARHDL"
     gene            complement(336..1106)
                     /locus_tag="VN041_13520"
     CDS             complement(336..1106)
                     /locus_tag="VN041_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWU30095.1"
                     /translation="MIDLAIASDVAHVTLNAPSKLNSLDEQALHDLGRAYDEAEQAGV
                     RALVLRGEGRAFCAGRDISGVDPRDDDVIGYLGGLVTPLLKRMAAFPAPTFAVAHGAC
                     LGVGLGLLIATDVVYVAESAKIGSPFAALGATLDSGGHALFLDRLGAHKTLDLIYTGR
                     LMTGAEAVASGLFSRVLPDDEVAQTTEDAATAASRGATAAFLASKQLVARIRDERLAL
                     WESVEIENAAQAALCDTDDYREGFAAFQQKRKPEFHGR"
     gene            complement(1145..2293)
                     /gene="paaE"
                     /locus_tag="VN041_13525"
     CDS             complement(1145..2293)
                     /gene="paaE"
                     /locus_tag="VN041_13525"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HWU30096.1"
                     /translation="MPDSRPHESAAVHPSGDRHRARFHTLTVSDVRHLTDDAIEVGFT
                     VPAELADDYAYLPGQYVALRVHLDGAEVRRSYSLCRPPVPVRDGATSLAVAVKRDEGG
                     LFSTWAQTELHPGFEIDVMSPQGTFTSGLEELEGMHVVGIAAGSGITPMMALARNLLT
                     ASETTRFTLLYTNKSTSDVMFLEDLADLKDRYPTRLVLHHVLSREQRTAPVLSGRIDE
                     EKLRTILDSLILPSTVDEWFLCGPFALVDLCREVLADIGVPREHVRFELFTTGDAPAE
                     RGPRQVEARAGEKAYRIEINLDGVSSTVESPVDAHESVLNAALRVRPDAPFACSGGVC
                     GTCRARVLEGSVTMTENYALEPDELERGYVLTCQSHPTSDRIVVDYDV"
     gene            complement(2308..2808)
                     /gene="paaD"
                     /locus_tag="VN041_13530"
     CDS             complement(2308..2808)
                     /gene="paaD"
                     /locus_tag="VN041_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWU30097.1"
                     /translation="MTVSDVTTARAREIAARVTDPEIPVLTIEDLGVLRDVRTDAAGR
                     VTVDITPTYSGCPAMDAIRDDVILALTAEGFEDVEVRLVLSPAWTTDWMSAEGKRKLT
                     EFGIAPPSGRAALGQGPIRLSLSVKCPRCGSLNTREVSRFGSTSCKALYECRACLEPF
                     DHFKVI"
     gene            complement(2814..3632)
                     /gene="paaC"
                     /locus_tag="VN041_13535"
     CDS             complement(2814..3632)
                     /gene="paaC"
                     /locus_tag="VN041_13535"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWU30098.1"
                     /translation="MSHDDVHVSVDELELASELSGGEAIAASADIAEYALWLADDALI
                     LSQQLGAWISRAPELEEDVALGNIALDLLGHARSLLRYAGSWDGRSEDDLAYFRDEAE
                     FRSAWLMEQPNGDFAQTIARQLLASAYMFELYSALRHSTDETLAAIAEKSLKEVDYHR
                     DHAVQWVLRLAGGTELSRERMIRALNDVWPYVDELFRDDPLIDRLGDAAARPSALRAG
                     FDAVIATVLAEAELETPTIGHSRAGGRHGAHSTPMGHLLAEMQVLARRHPGASW"
     gene            complement(3629..3934)
                     /gene="paaB"
                     /locus_tag="VN041_13540"
     CDS             complement(3629..3934)
                     /gene="paaB"
                     /locus_tag="VN041_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HWU30099.1"
                     /translation="MATPGGSPEETWPLWEVFVRANRGLSHVHVGSLHAPDASMAVRN
                     ARDLYTRRNEGVSIWVVPADAITTSDPDDKGAFFESPAGKNYRHAVYYTASEGVPHL"
     gene            complement(3934..4908)
                     /gene="paaA"
                     /locus_tag="VN041_13545"
     CDS             complement(3934..4908)
                     /gene="paaA"
                     /locus_tag="VN041_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWU30100.1"
                     /translation="MTAPADLSVVDDADALARFDELVANEQRIEPRDWMPEAYRKTLI
                     RQISQHAHSEIIGMQPEGNWITRAPSLKRKAILMAKVQDEAGHGLYLYSAAQTLGITR
                     DTMMQQLIDGKAKYSSIFNYPTPTWADMGAIGWLVDGAAIVNQVPLCRCSYGPYGRAM
                     VRVCKEESFHQRQGFEILLTLMQGTDEQREMAQDAVNRWYWPSLAMFGPPDDQSPNSA
                     QSMTWKIKRFSNDDLRQRFVGMLVPQAEILGITLPDPDLRWNDETQSYDMGEIDWDEF
                     FEVLKGNGPCNAERIERRRTAHEEGAWVREAAAEYARKQALRAEGQVA"
     gene            5012..>5283
                     /locus_tag="VN041_13550"
     CDS             5012..>5283
                     /locus_tag="VN041_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30101.1"
                     /translation="MLSPQSLSETDRRVVAAWAADCTERVLPCFERVAPDDDRPREAI
                     ARARAYARGDLDSAGEIRRRFVAGRAAASVASDAVAIAAARSAAQAS"
BASE COUNT          753 a         1765 c         1933 g          832 t
ORIGIN      
        1 gaaggtcatg ccgcgcggcg gcggatccgg aaccggacgc ggcggccagg gcatccagca
       61 tccggtcgac gtcggtgatg gtgaggacag ctgcggtggt gtgagtgacg tcgagcgagg
      121 tcagcgtgcg ccagcccact ccgagccggc cctgtcgcgg tgaggcgagc agcagtccga
      181 ccagtggcac gacctcgtcc gggtcggcgc gctccagcag ccgtgcgagc gcgtcgatct
      241 tggccagccg cgacgatgtc gccgcgacct cgtcggtggt ggcgaccagc tccgagagca
      301 tcatcccggc attgtcgcac cggtcaccga cgttgtcagc gtccgtggaa ctccggcttg
      361 cgcttctgct ggaaggcggc gaagccctcg cggtagtcgt ccgtgtcgca cagggcggcc
      421 tgagcggcgt tctcgatctc gaccgactcc cacagcgcca gccgctcgtc cctgattcgg
      481 gcgaccagct gcttgctcgc gaggaacgcc gccgtcgctc cgcgggaggc cgcagtcgcg
      541 gcatcctcgg tggtctgcgc gacctcgtcg tcggggagca cgcgagagaa caggccggat
      601 gccacggcct cggcgcccgt catgagccgg cccgtgtaga tgaggtcgag cgtcttgtgc
      661 gcgcccagcc ggtcgaggaa cagggcgtgc ccgccggagt cgagcgtcgc cccgagcgcc
      721 gcgaacgggg agccgatctt cgcggactcg gcgacgtaca cgacatccgt ggcgatgagc
      781 agtcccaggc cgacgcccag gcatgcgccg tgcgcgacgg cgaacgtcgg ggcggggaag
      841 gccgccatcc gcttgagcag cggggtgacc aggccgccga ggtagccgat cacgtcgtca
      901 tcccgcgggt cgacgccgga gatgtcacgg cccgcgcaga acgcccggcc ctcgccgcgc
      961 agcaccagcg cgcggacacc ggcctgctcg gcctcgtcgt acgctcggcc caggtcgtgc
     1021 agcgcctgct cgtcgagcga gttgagcttc gagggcgcgt tgagcgtgac gtgcgcgacg
     1081 tccgacgcga tggcgaggtc gatcatggtg ctccttcggt cgtgggcggg cctgggcggt
     1141 ccggtcagac gtcgtagtcg acgacgatgc ggtcgctggt ggggtgggac tggcaggtga
     1201 gcacgtagcc gcgctcgagc tcatcgggct ccagcgcgta gttctcggtc atggtcacgc
     1261 tgccctccag cacccgtgca cggcaggttc cgcacacgcc gcccgagcag gcgaaggggg
     1321 cgtccgggcg cacgcgcagc gcggcgttca gcaccgattc gtgcgcatcg accgggctct
     1381 ccaccgtcga ggagacgccg tcgagattga tctcgatgcg gtacgccttc tcgccggcgc
     1441 gcgcctccac ctggcgggga ccgcgctctg cgggcgcgtc accggtggtg aacagctcga
     1501 agcgcacatg ctcgcggggt acgccgatgt cggccagcac ctcgcggcac aggtccacca
     1561 gggcgaacgg tccgcacagg aaccactcgt cgaccgtgga cggcaggatg agcgagtcca
     1621 ggatcgtgcg cagcttctcc tcgtcgatgc gtccggagag caccggcgcg gtgcgctgct
     1681 cgcgcgagag cacgtggtgc agcaccagcc gggtcgggta gcggtccttg aggtccgcga
     1741 ggtcttcgag gaacatcacg tcgctggtgg acttgttcgt gtagagcagc gtgaagcgcg
     1801 tggtctcgga cgccgtgagc agattgcgcg cgagcgccat catcggcgtg atgccggagc
     1861 cggcggcgat gccgacgacg tgcatgccct cgagctcctc gagccccgag gtgaatgtgc
     1921 cctgcgggct catcacgtcg atctcgaacc ccggatgcag ttcggtctgc gcccacgtcg
     1981 agaacaggcc gccctcgtcg cgcttgacgg cgaccgccag gctggtcgcg ccgtcgcgca
     2041 ccggcaccgg cgggcggcac agcgagtacg agcggcgcac ctccgccccg tcgaggtgca
     2101 cccgcagcgc cacgtactga ccgggcaggt aggcgtagtc gtcggcgagc tcggcgggaa
     2161 ccgtgaagcc cacctcgatg gcgtcgtcgg tgaggtggcg cacgtcggac acggtcagcg
     2221 tgtggaaccg tgcgcggtgc cggtctccgc tggggtgcac ggcggcgctc tcgtgcgggc
     2281 gggaatcggg catccggaac agcgacatca gatcaccttg aaatggtcga agggctccag
     2341 gcacgcgcgg cactcgtaga gcgccttgca cgaggtggag ccgaagcggg acacctcgcg
     2401 ggtgttcagc gaaccgcacc gcgggcactt gacgctcagc gagaggcgga tgggcccctg
     2461 cccgagggcg gcccggccgg acggcggggc gatgccgaac tcggtgagct tgcgctttcc
     2521 ctcggcgctc atccagtcgg tggtccaggc gggggagagc accagccgca cctcgacgtc
     2581 ctcgaatccc tcggcggtga gcgcgaggat gacgtcgtcg cggatggcgt ccatcgccgg
     2641 gcatccgctg tacgtcggcg tgatgtcgac ggtgacgcgc cctgcggcat ccgtcctgac
     2701 atcgcgcagc acgccgaggt cctcgatggt caagacgggg atctcggggt cggtcacgcg
     2761 tgcggcgatc tcccgcgcgc gtgcggtggt gacatcggag acggtcatgg tgctcaccag
     2821 ctcgctcccg gatgccgtcg ggcgagcacc tgcatctcgg cgagcaggtg acccatcggc
     2881 gtcgagtgag cgccgtgccg cccgccggcc cgggagtggc cgatcgtcgg cgtctcgagc
     2941 tcggcctcgg cgagcacggt cgcgatcacg gcgtcgaaac cggcgcggag cgcggacgga
     3001 cgcgccgccg cgtcgcccag gcgatcgatc agcgggtcgt cgcggaacag ctcgtcgacg
     3061 tacggccaca catcgttcag cgcgcggatc atcctctcgc gggagagctc ggtgccgccg
     3121 gccaggcgca gcacccactg cacggcgtgg tcgcggtggt agtccacctc cttgagggac
     3181 ttctcggcga tcgcggcgag ggtctcgtcg gtcgagtggc gcagggcgga gtacagctcg
     3241 aacatgtagg cggatgccag cagctggcgc gcgatggtct gcgcgaagtc gccgttgggc
     3301 tgctccatga gccaggcgct gcggaactcg gcctcgtcgc ggaagtacgc caggtcatcc
     3361 tcgctgcgac cgtcccagga accggcgtac cgcagcagcg agcgggcgtg tccgagcagg
     3421 tccagggcga tgttgccgag ggcgacgtcc tcctccagct ccggtgcacg ggagatccac
     3481 gcacccagct gctgagacag gatcagtgcg tcatcggcga gccacagcgc gtactcggcg
     3541 atgtcggcgg aggcggcgat cgcctcgcct ccggacagct cggacgcgag ttccagctcg
     3601 tcgaccgaga cgtggacgtc gtcgtggctc acaggtgcgg caccccctcg gacgccgtgt
     3661 agtacacggc gtgccggtag ttcttgcccg ccgggctctc gaagaacgcg cccttgtcgt
     3721 cggggtcgct ggtggtgatg gcatccgccg gcacgaccca gatcgacacg ccctcgttgc
     3781 gacgggtgta caggtcacgg gcgttacgga ccgccatgct cgcgtcgggc gcgtgcagcg
     3841 agccgacgtg cacgtggctc aggccgcggt tcgcgcggac gaagacctcc cacagaggcc
     3901 aggtctcctc aggggagccg ccgggggtcg ccatcatgcc acctgtcctt ccgcacggag
     3961 ggcctgcttg cgggcgtact cggcggcggc ctcgcggacc caggcgccct cctcgtgggc
     4021 ggtgcgacga cgctcgatcc gctcggcgtt gcaggggccg ttgcccttga gcacctcgaa
     4081 gaactcgtcc cagtcgatct cgcccatgtc gtagctctgc gtctcgtcgt tccagcgcag
     4141 gtcgggatcg ggcagggtga tcccgaggat ctcggcctgc ggcacgagca ttccgacgaa
     4201 ccgctggcgc aggtcgtcgt tcgagaagcg cttgatcttc caggtcatcg actgcgcgga
     4261 gttgggggac tggtcgtcgg gcgggccgaa catggccagg ctcggccagt accagcggtt
     4321 cacggcgtcc tgcgccatct cgcgctgctc gtcggtgccc tgcatgaggg tcagcaggat
     4381 ctcgaagccc tgtcgctggt ggaaggactc ctccttgcac acgcgcacca tcgcgcggcc
     4441 gtaggggccg tacgagcacc ggcacagggg cacctggttc acgatggcgg cgccgtcgac
     4501 cagccagccg atcgccccca tgtcggccca ggtgggggtg gggtagttga agatcgacga
     4561 gtacttggcc ttgccgtcga tgagctgctg catcatcgtg tcgcgcgtga tgcccagggt
     4621 ctgcgcggcg gagtagaggt acaggccgtg gcccgcctcg tcctgcacct tggccatcag
     4681 gatggccttt cgcttcaggc tcggtgcgcg cgtgatccag ttgccctcag gctgcatccc
     4741 gatgatctcg gagtgcgcgt gctgggagat ctggcggatc agcgtcttgc ggtacgcctc
     4801 cggcatccag tcgcggggct cgatgcgctg ctcgttggcg acgagttcgt cgaagcgcgc
     4861 gagcgcgtcg gcgtcgtcca ccacggacag gtctgcgggt gcggtcatct tcgactcctc
     4921 ggcgtgtgat cttttactga ccgatcgttt ggtaattctc gcacggacgc ggatcgcgcg
     4981 caagccgatc gccgcgtgga aacatggagg ggtgctctcg cctcagtcgc tcagtgagac
     5041 cgaccgtcgc gtcgtcgcgg cgtgggctgc ggactgcacg gagcgcgtcc tgccctgctt
     5101 cgaacgggtg gcaccggatg atgaccgtcc gcgcgaggcg atcgcgagag cacgggccta
     5161 cgcccgagga gacctcgact ccgcaggaga gatccgtcgc cggttcgtgg cggggcgcgc
     5221 ggcggcgtcg gttgcgtcgg atgcggtggc gatcgcggcc gcacgctcgg ctgctcaggc
     5281 ctc
//
LOCUS       DATMSX010000423         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_176870,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000423 DATMSX010000000
VERSION     DATMSX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176870"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VN041_13555"
     CDS             complement(<1..396)
                     /locus_tag="VN041_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HWU30102.1"
                     /translation="MIIDLPDTTVSQVAKQLVKVREEGGAVALGRVLTLLIIARNGVA
                     EPAIDAANDASREHPMRVIVLITGEGEAHLDAQIRVGGDAGASEVVVLRAFGDAASNA
                     ESLVTGILLPDAPVVAWWPEEAPTDAANRS"
     gene            complement(393..>1883)
                     /gene="zwf"
                     /locus_tag="VN041_13560"
     CDS             complement(393..>1883)
                     /gene="zwf"
                     /locus_tag="VN041_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWU30103.1"
                     /translation="DRRLNRIAGPSSLIIFGVTGDLSRKKLMPAVYDLANRGLLPPGF
                     ALVGFARRDWADEDFEKVVYDAVKEYARTPFDDEVWQQLAQGIRFVSGEFDDDDAFRR
                     LKETVAQLDRDRGTMGNHAFYLSIPPKAFPLVTEQLRRSGLAEQNEGWRRVVIEKPFG
                     SDLKTARELNSVVESVFPADSVFRIDHYLGKETVQNILALRFANELYEPLWNRNYVDH
                     VQITMAEDIGVGGRAGYYDGIGAARDVIQNHLLQLLALTAMEEPISMSADHLRAEKEK
                     VLEAVRLPDDISLATARGQYAGGWQGGEKVNGFLEEDGMAPQSSTETYAAIKLEIDTR
                     RWAGVPFYLRTGKRLGRRVTEIAVVFKRAPEHLFGRAQTSELGQNALVIRVQPDEGVT
                     IRFGSKVPGSGANVRDVTMDFGYGHAFTEASPEAYERLILDVLLGDPPLFPRHEEVEL
                     SWKILDPVEQYWASLHGPLEQYAPGSWGPASADALLARDGRVWRRP"
BASE COUNT          270 a          641 c          663 g          309 t
ORIGIN      
        1 cgatctgttg gcggcatccg tcggtgcctc ctccggccac cacgccacca cgggcgcgtc
       61 ggggagcagg atgccggtga cgagactctc ggcgttgctc gcggcgtcgc cgaaggcgcg
      121 cagcacgacg acctcgctcg caccggcgtc tccgcccacg cggatctgcg cgtcgaggtg
      181 cgcctcgccc tcaccggtga tcagcacgat cacgcgcatg gggtgctcgc gggacgcgtc
      241 gttggccgcg tcgatcgctg gctccgccac gccgttgcgg gcgatgatga gcagcgtcag
      301 gacgcgaccg agggcgacgg cgccgccctc ctcgcgcacc ttcaccagct gcttggcgac
      361 ctggctgacg gtggtgtcgg gaaggtcgat gatcatgggc gtctccagac tcgtccgtcg
      421 cgggccagca gggcgtcggc ggatgccggg ccccaggaac cgggtgcgta ctgctccagt
      481 ggtccgtgca gcgaggccca gtactgctcg accgggtcga ggatcttcca ggacagctcg
      541 acctcctcgt ggcgggggaa cagcggtggg tcgccgagga gcacatccag gatcaggcgc
      601 tcgtacgcct cagggctggc ctcggtgaag gcgtggccgt atccgaagtc catggtcacg
      661 tcgcggacgt tggcaccgct gcccggcacc ttcgaaccga accggatcgt gacgccctcg
      721 tcgggctgca cgcggatcac cagggcgttc tggccgagct cagaggtctg ggcgcggccg
      781 aacaggtgct cgggagcgcg cttgaacacg actgcgatct cggtcacgcg gcggccgagc
      841 cgcttgccgg tgcgaaggta gaacggcacg ccggcccagc ggcgggtgtc gatctcgagc
      901 ttgatggccg cgtaggtctc cgtggaggac tgcggcgcca tgccgtcctc ctccaggaag
      961 ccgttgacct tctcgccgcc ctgccagccg ccggcgtact gcccgcgggc ggtggccagc
     1021 gagatgtcgt cgggcaggcg cacggcctcg agcactttct ctttctcggc gcgcaggtgg
     1081 tcggccgaca tgctgatcgg ctcctccatc gcggtgaggg cgagcagctg cagcaggtgg
     1141 ttctggatga cgtcgcgggc cgcgccgatt ccgtcgtagt agccggcacg accgcccacg
     1201 ccgatgtcct cggccatcgt gatctggacg tggtcgacgt agttgcggtt ccacagcggc
     1261 tcgtacagct cgttggcgaa gcgcagcgcc aggatgttct gcaccgtctc cttgccgagg
     1321 tagtggtcga tgcggaacac ggaatcggcc gggaacaccg actcgaccac ggagttcagc
     1381 tcgcgcgcgg tcttcaggtc actgccgaac ggcttctcga tgaccacccg gcgccagccc
     1441 tcgttctgct cggcgagccc actgcggcgc agctgctcgg tgaccagcgg gaacgccttg
     1501 ggcgggatcg acaggtagaa cgcatggttg cccatcgttc cccggtcgcg gtcgagctgc
     1561 gcgacggtct ccttcaggcg ccggaacgcg tcgtcgtcgt cgaactcgcc cgacacgaac
     1621 cggatgcctt gggccagctg ctgccagacc tcgtcatcga agggggtgcg cgcgtactcc
     1681 ttgacggcgt cgtaaacgac cttctcgaag tcctcgtcgg cccagtcgcg gcgggcgaag
     1741 cccaccagcg cgaagccggg cggcagcaac ccgcggttgg cgaggtcgta gaccgccggc
     1801 atcagtttct tgcgcgacag gtcccccgtc acgccgaaga tgatcaggct gctcgggccg
     1861 gcgatgcggt tgagccgccg atc
//
LOCUS       DATMSX010000424         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_186354,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000424 DATMSX010000000
VERSION     DATMSX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186354"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VN041_13565"
     CDS             <1..839
                     /locus_tag="VN041_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HWU30104.1"
                     /translation="YGAGPLPAVGMPESVQGDGAKSAAGGSAPGTSTDTPSAGRDIIT
                     TARVTLRVADIAKAADALTALAEKHGGYVESSDIGLEPGARSGTSPDAGQGAPTEPSL
                     PSGDGWVSIRVPAADLTAVMDAVGERGQVLGSSISREDVTATAVDLRARVDASKASVQ
                     RLTELMSKSGSVADLIAAETALSERQAELESYQQQLKSLDDQVSMSSVQVQLTKRAKA
                     TQADPAGFGDGLLAGWNGLIAALNGVVIAFGFLLPWLAVAGVVLLVVWLIRRRMRKTR
                     VP"
     gene            899..1435
                     /locus_tag="VN041_13570"
     CDS             899..1435
                     /locus_tag="VN041_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30105.1"
                     /translation="MEHISVITAIAHPLRRRMIDHLQLHGETQVGTFARVLDAQVGSI
                     SHHLRMLEKAGVVERVEAPDGDGRASWWRLARRSFTWSSNDYESPADALLAREAQRAN
                     VRHQLDLLQRWYRVSPQSEATGFSTDSLAWATQEELSDLQARLNAMVDEWRRGIDRED
                     GQERMPVFFFAHGFPTEV"
     gene            1437..2684
                     /locus_tag="VN041_13575"
     CDS             1437..2684
                     /locus_tag="VN041_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30106.1"
                     /translation="MTATEPTLLRTPPAFRRDRLVQAWLVIKALSDAGDAIFTIAFAW
                     TAVQIASPAVAGLVVAAGTVPRAIVLLVGGAYADRKDARRLMVLFNALRVVVLVAVAV
                     WCLAAQPTVAALLIASIAFGLCDAFFEPAAGTMPRQLVSTADLPAYSALSQTLSRLGT
                     MAGSAMGGFLVAAFGLSGSAGLNTLTYVVVIVFIVLWLRPRFALPRASAEQSVLRGVA
                     DGFRYLGRERATRTLVIALSGLNLAVGPAISIGLPLQATAHGWGAGAVGVFEALVGGG
                     AMAGALVVVRRRPRREAIGGFTALMVQGAGIVALGIGSPIVVGAGSLAIGFTAGFASA
                     LLGSTFAATAAPDYLGRLGSILRLGDDCLMPVAMALFGLIAGVLPLWVPFALYGGAMA
                     LLMIMPLRNPGIRAISLRDAATS"
     gene            2695..>3005
                     /locus_tag="VN041_13580"
     CDS             2695..>3005
                     /locus_tag="VN041_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWU30107.1"
                     /translation="MSSAAPTPTRVIPQGRDLTLDLVRVGCVLLVVFAHILYSGVGRG
                     SDGRILIEKTVEQQPWFDVASWIVDIMPLFFVIGGFAARAGWRSAQRRGAGEDQFVRV
                     RL"
BASE COUNT          385 a          993 c         1122 g          505 t
ORIGIN      
        1 cctatggtgc agggccgctt cccgcggtgg ggatgccgga gtccgtgcag ggcgacggag
       61 cgaagagtgc ggccggaggg agcgcgcccg gcacgagcac ggacaccccc tccgcgggcc
      121 gtgacatcat caccaccgcg cgggtcacgc tgcgcgtcgc cgacatcgcg aaggcggccg
      181 atgcgctgac cgctctcgcc gagaagcacg gcggctacgt ggagtccagc gacatcgggc
      241 tcgagccggg tgcacggtcc gggacctctc cggatgccgg gcagggcgca cccaccgagc
      301 cctcgctgcc gagcggggac gggtgggtca gcatccgcgt gcccgccgcc gatctcacag
      361 cggtgatgga tgccgtcggc gagcggggtc aggtgcttgg ctcctcgatc tcgcgggagg
      421 acgtcaccgc gaccgccgtc gatctgcgcg ctcgcgtcga tgcatcgaag gcgtcggtgc
      481 agcgcctcac cgaactgatg tcgaagtcgg gctcggtggc ggacctgatc gccgcggaga
      541 ccgcgctcag cgaacggcag gcagagctgg agagctacca gcagcagctg aagtcgctcg
      601 acgaccaggt gtcgatgtcc agcgtgcagg tgcagctgac caagcgcgcg aaggcgaccc
      661 aggcagatcc ggccggtttc ggcgacggac tgctggccgg ctggaacggg ctgatcgcgg
      721 cgctgaacgg ggtggtcatc gcgttcgggt tcctgcttcc ctggctcgcc gtcgccggcg
      781 tggtgctgct ggtggtctgg ctcattcgcc gtaggatgcg caagacccga gttccttgat
      841 tttccggggt atacaagaag tcttgtgaga atgcggaagc ccgcttagcg ttgcgggcgt
      901 ggaacacatc tccgtcatca ccgcgatcgc gcatccgctg cggcgtcgca tgatcgatca
      961 tctgcagctg cacggcgaga cccaggtcgg gacgttcgcg cgggtcctcg acgcgcaggt
     1021 gggcagcatc agccaccatc tgcggatgct ggagaaggcc ggcgtcgtcg agcgcgtgga
     1081 ggcgcccgac ggcgacggaa gggcgagttg gtggcggctc gccaggaggt cgttcacgtg
     1141 gtcgtcgaac gactacgagt ccccggcgga cgcccttctg gcccgtgagg cgcagcgcgc
     1201 gaacgtgcgc catcagctcg atctgctgca gcgctggtac cgggtgtcgc cgcagagcga
     1261 ggcgaccggc ttcagcacgg actcgctggc ctgggcgacg caggaggagc tcagcgacct
     1321 gcaggcgcgg ttgaacgcga tggtcgacga gtggcgccgc gggatcgacc gcgaggacgg
     1381 tcaggagcgg atgccggtgt tcttcttcgc ccacggcttt ccgacggagg tgtgagatga
     1441 ccgccaccga gcccaccctg ctgcgcactc cgcccgcgtt ccgtcgtgat cgtctggtgc
     1501 aggcgtggct ggtgatcaag gcgctgtcgg atgccgggga cgcgatcttc acgatcgcct
     1561 tcgcgtggac cgcggtgcag atcgcctccc ccgccgtcgc gggcctcgtg gtcgccgcag
     1621 gtacggtgcc gcgggcgatc gtgctgctgg tgggcggggc gtacgccgac cgcaaggacg
     1681 cccggcggct gatggtgctg ttcaacgcat tgcgcgtggt cgtgctcgtc gcagtcgccg
     1741 tctggtgcct ggcggcgcag cccaccgtcg cggcgctgct gatcgcgagc atcgcgttcg
     1801 gcctgtgcga cgcgttcttc gagcccgcgg cggggacgat gccccggcag ctcgtatcca
     1861 ccgccgatct tcccgcctac tcggcactca gccagacgct gtcccggctg gggacgatgg
     1921 ccggctcggc gatgggtggc ttcctcgtgg ccgcgttcgg gctgtcgggc agcgccgggc
     1981 tgaacacgct gacctacgtg gtggtgatcg tgttcatcgt gctctggctg cgcccgcgct
     2041 tcgcgctccc ccgcgcgtcg gcggagcaga gcgtgctgcg cggtgtcgcc gacggattcc
     2101 gctacctggg ccgggaacgc gccactcgca cactggtcat cgccctgtcg ggcctcaacc
     2161 tggcagtggg gccggcgatc tcgatcggcc tgccgctgca ggcgacggcc catgggtggg
     2221 gcgcaggagc ggtgggcgtg ttcgaggccc tggtcggcgg cggcgcgatg gccggtgcgc
     2281 tggtggtcgt gcgacgtcgc ccgcgccgcg aggccatcgg aggcttcact gcgctgatgg
     2341 tgcagggcgc gggaatcgtc gctctcggca tcgggtcgcc gatcgtcgtg ggcgcgggga
     2401 gcctggcgat cggattcacc gcaggcttcg cctcagccct gctggggtcg acgttcgccg
     2461 cgactgcggc ccccgactac ctggggcgac tcgggtcgat cctgcggctc ggtgatgact
     2521 gcctgatgcc ggtcgccatg gcgctgttcg gcctgatcgc gggcgtcctg ccgctctggg
     2581 tgcccttcgc gctgtacggg ggtgcgatgg cgctgctgat gatcatgccg ctgcgcaacc
     2641 ccggcatccg ggcgatctcc ttgcgggacg cggccacctc ctagactcat cggcatgagc
     2701 agcgccgcac cgacgccgac ccgtgtcatc ccgcagggtc gggatctcac gctcgacctg
     2761 gtgcgcgtgg gctgcgtgct gctggtcgtg ttcgcgcaca tcctgtacag cggcgtcggc
     2821 cggggttctg acggccggat cctcatcgag aagaccgtcg agcagcagcc gtggttcgat
     2881 gtcgcgagct ggatcgtcga catcatgccg ctgttcttcg tgatcggcgg cttcgcagcc
     2941 cgcgccggat ggcgctccgc gcagcggcgc ggtgccggcg aggatcagtt cgtgcgcgtg
     3001 cggct
//
LOCUS       DATMSX010000425         6138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72809,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000425 DATMSX010000000
VERSION     DATMSX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72809"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VN041_13585"
     CDS             complement(<1..306)
                     /locus_tag="VN041_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU30108.1"
                     /translation="MRPIPRRSLVDEAIEQFREQIDNGSWPVGHRLPAEARLAEELGM
                     SRASVREATRALVHAGLLSSRQGDGTFVIATDESDVALRRQLGAAEVVEVIEVRRGLD
                     "
     gene            complement(450..914)
                     /gene="rraA"
                     /locus_tag="VN041_13590"
     CDS             complement(450..914)
                     /gene="rraA"
                     /locus_tag="VN041_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HWU30109.1"
                     /translation="MISTADLYDLHGDALQSLALQLRSFGGLLRFEGPIRTVRCFQDN
                     ALVKQILQTPGDGAVLVVDGAGSLETALMGDMIAQSAVDSGWAGVVINGCVRDSVALL
                     GMPLGVKALGTNPRKSAKEGAGEVDVVVEFGGVTFRPGALLHADEDGVLVER"
     gene            complement(1022..2617)
                     /locus_tag="VN041_13595"
     CDS             complement(1022..2617)
                     /locus_tag="VN041_13595"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HWU30110.1"
                     /translation="MTHYQDDIAAIQELKNANGSAWNAIDPESAARMRAQNRFRTGLE
                     IAQYTADIMRRDMAEYDADSSVYTQSLGVWHGFIGQQKLISIKKHLKSTNKRYLYLSG
                     WMVAALRSEFGPLPDQSMHEKTVVPALIQELYTFLRQADARELDLLFTQLDAARAAGD
                     ETAAEFIQSQIDGFETHVVPIIADIDAGFGNPEATYLLAKKMIEAGACAIQIENQVSD
                     EKQCGHQDGKVTVPHEDFIAKLNAVRYAFLELGIDNGIIVARTDSLGAGLTQKLAVSQ
                     APGDLGDQYNSFLDVEEIGEAELGDGDVVIKREGKLLRPKRLASNLYQFRQGTGVDRV
                     VLDCITSLQNGADLLWIETEKPHVEQIAGMVDRIRKVVPNAKLVYNNSPSFNWTLNFR
                     QQAYDQLVEQGQDVSAYDRDKLMSVDYDDTELARIADEKIRTFQRDGSARAGIFHHLI
                     TLPTYHTAALSTDNLAKGYFGDEGMLTYVRDVQRQEIRQGIATVKHQNMAGSDLGDNH
                     KEYFAGDAALKAGGQHNTMNQFS"
     gene            complement(2646..4319)
                     /gene="aceB"
                     /locus_tag="VN041_13600"
     CDS             complement(2646..4319)
                     /gene="aceB"
                     /locus_tag="VN041_13600"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HWU30111.1"
                     /translation="MSIATAAQTDQIRIVDTVASARPELGPALRITGPLNDRFDEILT
                     PDALAFLTELHHRFAGRRLDRLADRMRRRFEIGNGHDPRFREDTAHIREDADWRVAGA
                     GPGLEDRRVEITGPTDPKMTINALNSGARVWLADQEDATSPTWGNVIGGQLSLRDAIR
                     GELTHTSADGREYRVTAERTPTIVMRPRGWHLGEKHIDFIDRTGRTLSASGSLVDFGL
                     YFFHNAQALIDAGRGPYFYIAKLESSEEARLWNDVFSFSEEYIGIPHGTIRATVLIET
                     LPAAFEMDEILYELRDHCAGLNAGRWDYIFSIIKNYRGRGARFVLPDRSEVTMTVPFM
                     RAYTELLVKTCHQRGAHAIGGMSAFIPNRRDPEVTERALEKVAADKRREAGDGFDGTW
                     VAHPDLIPAAQAEFDAVLGDRPNQIDRQRDDVHVTAAQLLDLRIGRPITEKGVRDNVS
                     VAIRYIEAWLRGLGAVALDDLMEDAATAEISRSQVWQWIHQDRRTEEGDLITADYVER
                     LIAEMLAQTPRTEGDRFDDAAEVFREVALHPEFPAFLTLPASRFLTETD"
     gene            4450..5901
                     /locus_tag="VN041_13605"
     CDS             4450..5901
                     /locus_tag="VN041_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30112.1"
                     /translation="MASTDAPEESDALTIGRRIRQLRTAQGMTLEELATRIDRAPSQV
                     SMFETGKREPKLTLLQSIARALGTTIDALLEGEPLDERSTMEIALERAMKGQTFQALG
                     IPSFRIGKSMPDQALAAMLALHGEIERLRDERAATPEEARRANVELRHLMRSQDNYFA
                     DLEAQAADILAAVGHPGGPITQRTASEIAAHLGFTLHYANDLPQTTRSVADLAHGRLY
                     LSGNVQAKGDARTAVLQALSSRILGHGEPRSYAEFLRQRVETNYLTGAILVPESHVVP
                     VLQEAKSRRAISVEDLRDAYSVSYETAAHRFTNLATRHLDIPVHFLKVHESGTITKAY
                     ENDDVNFPTDRLGAIEGQMCCRRWTSREVFEVEDRFNPYYQYTDTGNGTYWCTARVEP
                     SSEGLHSVSVGVRFDDTKWFVGRDTTNRGVSKHSVEVCCRRAPAELEERWREQSWPNV
                     RTPRTLLATLPTGSFPGVDTTDVYEFLEAHAPR"
     gene            complement(5905..>6138)
                     /locus_tag="VN041_13610"
     CDS             complement(5905..>6138)
                     /locus_tag="VN041_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HWU30113.1"
                     /translation="QAGPTPASAEPLLTALMTAIAAAVPGDPLPAARTFTAWIFGFCT
                     MEQAGAFRLGGDVDEAFEFGLDALLGAIGARRV"
BASE COUNT          899 a         2024 c         2199 g         1016 t
ORIGIN      
        1 gtccagaccg cggcgcacct cgatgacctc gacgacctcg gcggcgccga gctgccggcg
       61 cagggccaca tcggactcgt cggtggcgat cacgaatgtg ccgtcgccct gacgggagga
      121 gagcagcccc gcgtgcacca gcgcgcgcgt cgcctcgcgg acggacgccc ggctcatgcc
      181 gagctcctcg gccagcctgg cctcggcggg cagacgatgc cccacgggcc agctgccgtt
      241 gtcgatctgc tcgcggaact gctcgatggc ctcatcgacg agcgaccggc gggggatggg
      301 gcgcatgggg tcctcccggg agtgaggcgg tcatcgaagc cgaccgccgc gggcgtcgat
      361 gaggtcgagg cgcccgcgca gaccaccacg tgatctcatc agcacactac ccagtcatcc
      421 ggccgcccga caatgcgcgc gcatcgcgct cagcgctcga cgaggacgcc gtcctcatcg
      481 gcgtgcagca gtgcgcccgg gcggaaggtc acgccgccga actcgaccac gacgtcgacc
      541 tctcctgcgc cctccttcgc gctcttgcgc gggttggtgc cgagcgcctt gacgcccagt
      601 ggcatcccga gcagcgccac gctgtcgcgc acgcatccgt tgatcacgac gccggcccag
      661 cccgagtcca cggcactctg cgcgatcatg tcgcccatca gcgcggtctc cagggatccg
      721 gctccgtcca cgacgagcac cgcgccgtcg cccggtgtct gcaggatctg cttcaccagc
      781 gcgttgtcct ggaagcaccg cacggtgcgg atggggccct cgaagcggag cagaccgccg
      841 aacgagcgca gctgcagggc gagggactgc agggcgtcgc cgtgcaggtc atagaggtcg
      901 gcggtggaaa tcatggggtc tccttcggat gtgcgggcgg tacagatgaa cgacgcggga
      961 acgcctgcac gatcggaagg ggcttccggt cgtgcaggcg ttccacggtc gtgcagctgt
     1021 gtcagctgaa ctggttcatg gtgttgtgct ggccgccggc cttgagggcg gcgtcgccgg
     1081 cgaagtactc cttgtggttg tcgccgaggt cgctgccggc catgttctgg tgcttgaccg
     1141 tggcgatgcc ctggcggatc tcctggcgct gcacgtcgcg gacgtaggtc agcatgccct
     1201 cgtcaccgaa gtagcccttg gccaggttgt cggtcgacag cgcggccgtg tggtacgtcg
     1261 gcagggtgat caggtggtgg aagatgccgg cgcgggcgga cccgtcgcgc tggaacgtgc
     1321 ggatcttctc gtcggcgatg cgggcgagct cggtgtcgtc gtaatcgacg ctcatcagct
     1381 tgtcgcggtc gtacgcggag acgtcctgac cctgctcgac gagctggtcg taggcctgct
     1441 gccggaagtt gagcgtccag ttgaacgacg ggctgttgtt gtacaccagc ttggcgttcg
     1501 gcacgacctt gcggatgcgg tcgaccatcc cggcgatctg ctcgacgtgc ggcttctcgg
     1561 tctcgatcca cagcaggtcg gcgccgttct gcagcgaggt gatgcagtcc agcaccacgc
     1621 ggtcgacacc cgtgccctga cggaactggt agaggttgct cgccaggcgc ttcggccgca
     1681 gcagcttccc ctcgcgcttg atgaccacat caccgtcgcc cagctcggcc tcgccgatct
     1741 cctccacgtc aaggaacgag ttgtactggt cgcccaggtc gccgggcgcc tggctgacgg
     1801 cgagcttctg ggtgaggccg gcgcccagcg agtcggtgcg ggcgacgatg atcccgttgt
     1861 cgatgccgag ctcgaggaac gcgtagcgga cggcgttgag cttcgcgatg aagtcctcgt
     1921 gcggaaccgt gaccttgccg tcctgatgac cgcactgctt ctcgtccgag acctggttct
     1981 cgatctggat ggcgcacgcg cccgcctcga tcatcttctt ggccagcagg tacgtcgcct
     2041 cggggttgcc gaaaccggcg tcgatgtccg cgatgatcgg cacgacgtgc gtctcgaaac
     2101 cgtcgatctg ggactggatg aactccgccg cggtctcgtc tccggccgca cgtgcggcat
     2161 ccagctgggt gaacagcagg tcgagctcac gggcatccgc ctggcggagg aacgtgtaca
     2221 gctcctggat cagggcgggg acgacggtct tctcgtgcat ggactgatcg gggagcggcc
     2281 cgaactcgga gcgcagggcc gcgaccatcc agcccgagag gtagaggtag cgcttgttcg
     2341 tcgacttcag gtgcttcttg atcgagatca gcttctgctg cccgatgaag ccgtgccaga
     2401 cgccgagcga ctgggtgtag accgatgagt cggcgtcgta ctcggccatg tcgcggcgca
     2461 tgatgtcggc cgtgtactgc gcgatctcga ggccggtgcg gaagcggttc tgcgcccgca
     2521 ttcgtgcggc cgactcgggg tcgatggcgt tccacgcgga gccgttcgcg ttcttgagct
     2581 cctggatggc ggcgatgtcg tcctggtagt gagtcatgtc atgtccttct cagtggggcg
     2641 ggtcgtcagt cggtctcggt caggaagcgg gatgccggga gcgtcaggaa cgcggggaac
     2701 tccgggtgca gggcgacctc gcggaacacc tcggcggcgt cgtcgaagcg gtcgccctcg
     2761 gtgcgcgggg tctgcgcgag catctcggcg atcaggcgct cgacgtagtc ggcggtgatc
     2821 aggtcgccct cctcggtgcg gcggtcctgg tggatccact gccacacctg ggagcggctg
     2881 atctcggcgg tcgcggcgtc ctccatcagg tcgtcgaggg ccacggcgcc caggccccgc
     2941 agccatgcct cgatgtagcg gatggcgacc gacacgttgt cgcgcacgcc cttctcggtg
     3001 atcgggcgtc cgatgcgcag gtccagcagc tgagcggccg tcacgtggac gtcgtcgcgc
     3061 tgccggtcga tctggttcgg gcggtcgccg agcacggcgt cgaactccgc ctgcgcggcc
     3121 gggatcaggt cggggtgggc gacccaggtg ccgtcgaagc cgtcgccggc ctcgcgccgc
     3181 ttgtcggcgg ccaccttctc cagggcgcgc tcggtgacct cggggtcgcg gcggttcggg
     3241 atgaaggcgc tcatgccgcc gatggcgtgg gccccgcgct ggtggcaggt cttcaccagc
     3301 agctcggtgt aggcccgcat gaacggcacg gtcatggtga cctcgctgcg gtcggggagc
     3361 acgaagcgcg caccgcggcc gcggtagttc ttgatgatcg agaagatgta gtcccagcgg
     3421 ccggcgttga gccctgcgca gtggtcgcgc agctcgtaga ggatctcgtc catctcgaac
     3481 gcggcgggca gcgtctcgat cagcacggtc gcgcggatcg tgccgtgcgg gatgccgatg
     3541 tactcctcgc tgaacgagaa gacgtcgttc cacagccgcg cctcctcgct ggactccagc
     3601 ttggcgatgt agaagtacgg gccgcggccg gcgtcgatca gcgcctgcgc gttgtggaag
     3661 aagtacaggc cgaagtccac cagggagccg gatgcggaca gcgtgcgacc tgtgcggtcg
     3721 atgaagtcga tgtgcttctc gccgaggtgc cagccgcgcg ggcgcatcac gatcgtgggg
     3781 gtgcgctccg ccgtgacgcg gtactcgcgg ccgtcggcgc tggtgtgggt cagctcgccg
     3841 cggatcgcgt cgcgcagcga cagctgaccg ccgatgacgt tgccccaggt ggggctggtg
     3901 gcgtcctcct ggtcggccag ccacacgcgg gcgccggagt tgagggcgtt gatggtcatc
     3961 ttggggtcgg tggggccggt gatctccacg cggcggtcct ccaggccggg gccggcaccg
     4021 gcgacgcgcc agtcggcgtc ctcgcggatg tgcgcggtgt cctcgcggaa gcgggggtcg
     4081 tggccgttgc cgatctcgaa gcggcggcgc atccggtcgg cgaggcggtc gaggcggcgc
     4141 ccggcgaagc ggtggtgcag ctccgtgagg aacgccaggg catccggcgt caggatctcg
     4201 tcgaaccggt cgttcagggg cccggtgatg cgcagcgcgg gaccgagctc gggtcgggcg
     4261 gatgcgacgg tgtcgacgat cctgatctgg tcggtctgtg cggcagtggc gatgctcatg
     4321 atctgtctcc tccgtgagat gggatgccgg gtggcttgtc atccactgtg ggtgaagatt
     4381 caggtgccaa ctgaccattt ctggggagaa gttttctgga atttctgttt ctgtgacaca
     4441 cttgccccca tggccagcac ggatgcccca gaagagtccg acgccctcac gatcgggcgg
     4501 cggatcagac agctccgcac cgcgcagggg atgactctcg aggagctcgc gacgcgcatc
     4561 gaccgcgcgc ccagccaggt ctcgatgttc gagaccggga agcgcgagcc caagctcacc
     4621 ctgctgcagt cgatcgcccg cgccctgggc acgacgatcg acgcgctgct cgagggtgag
     4681 ccgctggatg agcgcagcac gatggagatc gcgctggagc gcgcgatgaa gggccagacg
     4741 ttccaggcgc tcggcatccc ctccttccgc atcggcaagt cgatgcccga tcaggcgctg
     4801 gccgcgatgc tcgccctgca cggcgagatc gagcggctgc gcgacgaacg ggccgccacc
     4861 cccgaggagg cgcggcgggc caatgtcgag ctgcggcacc tcatgcgcag tcaggacaac
     4921 tacttcgcgg atctggaggc ccaggcggcc gacatcctcg cggcggtggg gcatcccggt
     4981 gggccgatca cgcagcgcac ggcatccgag atcgccgcgc atctcggctt cacgctgcac
     5041 tacgcgaacg atctgccgca gacgacccgc agcgtcgccg acctcgcgca cggccggctg
     5101 tacctctccg gcaacgtgca ggccaagggc gacgcccgca ccgccgtgct gcaggcgctg
     5161 tccagtcgca tcctgggcca tggcgaaccc cgaagctacg ccgagttcct gcggcagcgc
     5221 gtggagacca actacctcac cggggcgatc ctcgtgcccg agtcgcacgt cgtccccgtg
     5281 ctgcaggagg cgaagtcccg tcgtgcgatc tcggtcgagg atctgcgcga cgcctactcc
     5341 gtgtcttacg agacggccgc gcaccgcttc acgaacctcg cgacgcggca tctggacatc
     5401 cccgtgcact tcctcaaggt gcacgagtcc ggcacgatca ccaaggcgta cgagaacgac
     5461 gatgtgaact tccccaccga ccgcctgggc gcgatcgagg ggcagatgtg ctgccggcgc
     5521 tggaccagcc gcgaggtgtt cgaggtggag gaccgcttca acccgtacta ccagtacaca
     5581 gacaccggca acggcaccta ctggtgcacg gcccgggtcg agccctccag cgaggggctg
     5641 cactcggtga gcgtgggcgt gcgcttcgac gacacgaagt ggttcgtcgg ccgcgacacg
     5701 accaatcggg gcgtctcgaa gcactccgtc gaggtgtgct gccgtcgtgc gcccgccgag
     5761 ctcgaggagc gctggcgcga gcagtcctgg ccgaacgtgc gcaccccgcg caccctgctg
     5821 gcgacgctgc cgaccggctc gttccccggc gtcgacacga ccgacgtgta cgagttcctc
     5881 gaggcgcacg ccccgcgctg atcgtcagac gcgtcgcgca ccgatggcgc ccagtagggc
     5941 gtcgagcccg aactcgaacg cctcgtcgac gtcgccgccg agacggaagg cgccggcctg
     6001 ctccatggtg cagaagccga agatccaggc ggtgaaggtg cgtgctgccg ggagggggtc
     6061 tccggggacg gcggcggcga tcgcggtcat cagcgcggtg agcagcggct cggcggatgc
     6121 cggagtcggg ccggcctg
//
LOCUS       DATMSX010000426         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_148002,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000426 DATMSX010000000
VERSION     DATMSX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148002"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VN041_13615"
     CDS             complement(<1..408)
                     /locus_tag="VN041_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWU30114.1"
                     /translation="MDTSAATVGMIGLGSMGAPVAKRILAARGELVIHARRPQPELVE
                     AGATWSVTPRDLASRVDVLLSLLPDLPELEALLEGEDGLLAGAGDLLILIGSTSSAPG
                     PACSVDDRNAGKRLVAISWPVSEKKLAAPTLATP"
     gene            507..1565
                     /locus_tag="VN041_13620"
     CDS             507..1565
                     /locus_tag="VN041_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWU30115.1"
                     /translation="MTQTEDRLRVVAAVPLDEELCRLIERIEPRVEMISDLALVPPMR
                     WPADWEGDPAFSRTPQQQADYEAMVDSADALFGIPDVSPAHLARTVAANPRLRWVMTT
                     AAGGGGQIKAAGLDQDALDRIVFTTSAGVHAGPLAEFAVFGVLAGAKGLPRLRADQDA
                     RVWPQRWEMRQIDEMTVLVVGLGGIGAECARRFHALGATVWGTTRSGRPVDGVSRLIA
                     PDEIALVAGEVDAIVVTLPGTEQTHHLIGEQALTAIRPGAILASVGRGTVIDERALLA
                     ALDDGRIGFAAMDVFEKEPLAQDSPLWSHPSVLVSPHTAARSSKEAERIARRFAESAG
                     RLLDGEPLRATVDTVEFY"
BASE COUNT          253 a          579 c          637 g          245 t
ORIGIN      
        1 tggcgtagcg agcgtcggcg ccgcgagctt cttctcggac accggccacg agatcgcgac
       61 gagcctcttg cccgcgtttc tgtcgtcgac gctgcacgcc gggccgggcg cggaggaggt
      121 cgagccgatc aggatgagca gatcgccggc gcccgcgaga agtccgtcct cgccctccag
      181 cagcgcctcg agctcgggga ggtccggcag caggctcagc agcacatcca cccgcgacgc
      241 gaggtcgcgg ggagtgacgg accacgtcgc ccccgcctcg acgagctcgg gctgcgggcg
      301 cctggcatgg atgacgagct cgccgcgtgc ggcgaggatc cgcttcgcga cgggtgcgcc
      361 catcgaaccc agtccgatca tcccgacggt ggcagcagag gtgtccatgc ggtcatgcta
      421 gcgaagatcc actcaatgga acagtgttca acggagtgga tttgcggtac ggtggtggct
      481 gatgtcaccc gaggaagggg atcacgatga cgcagaccga ggacaggctc cgagtggtcg
      541 cggctgtgcc gctggacgag gagctctgcc ggctcatcga gcgtatcgaa ccccgggtgg
      601 agatgatcag cgatctcgca ctggtcccgc cgatgcgctg gcccgccgac tgggagggcg
      661 atcccgcgtt ctcccgcacg ccgcagcagc aggctgacta cgaggcgatg gtcgactcgg
      721 cggatgccct gttcggcatc cccgacgtgt cgccggcgca cctcgcccgc accgtcgccg
      781 ccaatccgcg cctgcgctgg gtgatgacga cggcggccgg gggtggcggt cagatcaagg
      841 cggcgggtct cgatcaggac gccctggatc gcatcgtctt caccaccagt gccggcgtgc
      901 acgccgggcc gctcgcggag ttcgcggtgt tcggggtgct ggccggtgcg aagggtctgc
      961 cccgtctgcg cgccgaccag gatgcccgtg tgtggccgca gcgctgggag atgcgtcaga
     1021 tcgacgagat gaccgtgctg gtcgtgggcc tcggcggcat cggggcggaa tgcgcacgcc
     1081 gcttccatgc cctgggggcg acggtctggg gcacgacgcg ctccggacgc cccgtcgacg
     1141 gggtgagcag gctcatcgcc cccgacgaga tcgcgctggt cgccggcgag gtggatgcga
     1201 tcgtcgtgac cctccccggc accgagcaga cccatcatct gatcggcgag caggcgctca
     1261 cggccatcag accgggagcg atcctcgcca gcgtcggccg gggcaccgtg atcgacgagc
     1321 gtgcactgct ggcggcgctc gacgacggcc ggatcggctt cgccgcgatg gacgtcttcg
     1381 agaaggaacc gctcgcgcag gactcgccgc tgtggtcgca cccgagcgtg ctcgtgagcc
     1441 cgcacacggc cgcgcgcagc tcgaaggagg cggagcgcat cgcccggcgc ttcgcggaga
     1501 gcgccggccg tctcctggac ggcgagcctc tgcgcgccac ggtcgataca gtcgagttct
     1561 actgagttca cggcgcccgt gcgggcgccg ggagggggaa gcgtggcagg cgacgaggat
     1621 tcgcgtgaag gccgggatcc cgcaccggcg gtgacccgca gcatccggct gctgggactg
     1681 ctggcgcagg cgcagccgcg cacgctcacg gagc
//
LOCUS       DATMSX010000427         7009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_186427,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000427 DATMSX010000000
VERSION     DATMSX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186427"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VN041_13625"
     CDS             complement(<1..802)
                     /locus_tag="VN041_13625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30116.1"
                     /translation="MSDPQLTVCIVTYERPEFLARCLASLQRNAPDTAVVVVDASRVD
                     GAAAVAHPGVLYLHRPGLAGWMTRSRNEALLHAGSEIIAFLDDDVVVSEGWRQSLLDA
                     FSSDLRPDAVAGRTRNLQPGEDQYDLPIGRLREDGTLTAGFAAVRDGLEVVDHGIGAN
                     MSFRREMLARLGGFRDDYPGTAMREDTDMFLRVKRVGGVTVFAPLAVVDHLPAPHVKG
                     RRFDTRYKLYGRRNHMVMLARYSGWRSPLLRRWIASDLRAVLRAGGPLR"
     gene            863..1771
                     /locus_tag="VN041_13630"
     CDS             863..1771
                     /locus_tag="VN041_13630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWU30117.1"
                     /translation="MAERRVTVIIPTNRGGAYLDAAVASVRSQTRPAHEILLVDDGSP
                     EPGLADVAARLGLRYLRQDASGLSVARNTGVAGAEGEWIAFLDDDDVWHPERLEEQLR
                     ALDTEGDVVAVSTGGWYMDAEGVPFGTGWGAPPATSAQFIAYEAVPPRITTLLIRRSE
                     YLAVGGCRRAMEPAEDNDLIQRLLQRGEFASVDRQLVGYRRHTGNITQRSLAGREASR
                     IVIDDLLRDARHRQDGEVVALLKRQRRTARRISASGNTGELIAALRAGESGYAFQLAM
                     WGLRHVPFHSVAAVARRWRSREASGR"
     gene            1768..2796
                     /locus_tag="VN041_13635"
     CDS             1768..2796
                     /locus_tag="VN041_13635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30118.1"
                     /translation="MTSAREEGTDGRAAGRDGGAQTFTLIIPTHRRPGFVVQAVDSAL
                     RQERAFDRIIVIPDGLDDPAPDALAGLPVDVHPVAHGGVSAARNAGLALADTDWVCFL
                     DDDDLLHPAYLADLEAAVGAHPETSAFNAPYWSFAAVAGPHEEFSATSLDECLSASGS
                     AVPRNDMSYLEITGRSYDALLEKLAGSMSTAAVRTAVLREAGGFPVDLIAAEDWTMYV
                     NVARLTEWRVLDERRAFFRAHGDNAMSGRSRGKMLAPLRAIRSFWRSGQPSPPHAPLE
                     AYRRAYRNEVMTALAGCRRNGDAEGREEALRIAAEILPRRWDRIFIRMPRPVRSVMWR
                     LERGSRAS"
     gene            complement(2815..4614)
                     /locus_tag="VN041_13640"
     CDS             complement(2815..4614)
                     /locus_tag="VN041_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30119.1"
                     /translation="MTRQTRMSRLRLLLEVTGAAPARWVTTTVLVSLVLAGLDMVGVL
                     SMLPLMQLITSGKTDGAFLTWISTTIGTTDVSVLLPVIAGLVLIAFLIKSIGALMFRW
                     WLLGRTTRVSALAAAALMRRYVLAPYGSHRKRASSEIYRNINDATGQAASVLLAQVSL
                     VADVVTMTAIILVLAWSSLLATVFAGFLFGVIVFGVQRLLRRRQVNLGEEVAASSLQA
                     WQSLMPGIEGFRDTRLTASSELFVNRFRAARLRGARAQQQAGILGDVPRYLLEIAFLV
                     AIAGIAVILSVTGAQDQILGVLGVFAAASMRLLPALTRVTANVATTRAGDAGLRIMVS
                     ALDELDTEGQHDETPQAPTPPTGDIVVNEVSFHFPDSTQNVLDRVSVRIPENRTTAFV
                     GASGAGKSTLLDLLLGLLSPTAGDIRCGDQIIDDDLAAWHAGLGVVPQDVFISNATLA
                     ENVAFGLERSAIDDDRVREAVRMSLLDEVVAELPDGLDTMLGDRGTRLSGGQRQRVGL
                     ARALYRRPNVLVLDEATSALDNATEHEIAQTLQRLQGSMTIIVVAHRLSTVRHVDQLV
                     FLRRGEVAATGTFEQVRAADAEFARLVELGSLQ"
     gene            4834..5397
                     /locus_tag="VN041_13645"
     CDS             4834..5397
                     /locus_tag="VN041_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30120.1"
                     /translation="MSENTKTAGDGISRRTVTKAMAWSVPAIAVAAPIPAFAASGKPP
                     TVLPGKVFKWPGGSCVNTPSGLDNNKAYLFTFRVTNTSNKPIYIYSVTITTTSGITFN
                     TVGMTPGLGTPLAPNQSTDVSVWANSSDSGNLSFQATATITWGHNYPGPDPDNHAPIV
                     KTWQVNGTPTTTSQGYSQCQNPGYIGL"
     gene            complement(5533..6630)
                     /locus_tag="VN041_13650"
     CDS             complement(5533..6630)
                     /locus_tag="VN041_13650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30121.1"
                     /translation="MTDLVVLSLERWDGVWRRNQYLVAGLLDADPELRVLFVEPPDDP
                     IHDLRHRRRPSTGRGLTRIDDRLWTLRPVKWLPRRIDRGADDRLASSVRAAVETLRMP
                     HPVLWINDPAAVQLAQRTGWPVLYDMTDDWLAAERPESAQQRIAEGEKWLLQHAAAVV
                     ACSRELIRRKRSQRDDIVLVRNGVDTARYLSPAPRPADAPEGPYALYLGTLHRDRIDV
                     DLCEATATALRGKGHLVLVGPEAWSAADGERVRAAGAIVLGARDREAVPGYLQHADVL
                     VVPHLVTAFTESLDPIKLYEYQAVGRPIVSTPVAGFRDEPGVTIASGGDFASAVASTL
                     PAASAFPEGANARAADWSTRVAAFRAALPLR"
     gene            complement(6627..>7009)
                     /locus_tag="VN041_13655"
     CDS             complement(6627..>7009)
                     /locus_tag="VN041_13655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30122.1"
                     /translation="SDAVRLLGFREDLPARLERAALFLAPCEVEGLGLALIEAMGAGA
                     APVAASAAGHLDVLQGLDARSGYRPGDIPDAADALRTLAEDEERRMRLAEQAGERART
                     EFSLAQQSARTDDVYRRAIERRLR"
BASE COUNT         1090 a         2465 c         2486 g          968 t
ORIGIN      
        1 accgcagggg tccgcccgcg cggagcacgg cccgcaggtc cgacgcgatc cagcggcgca
       61 gcagcggcga gcgccagccg gagtagcgcg cgagcatgac catgtggttc cggcgcccgt
      121 acagcttgta ccgggtgtcg aagcgccgtc ccttcacatg cggcgccgga agatgatcga
      181 ccacggcgag cggggcgaac acggtcaccc ctcctacccg cttgacccgc aggaacatgt
      241 cggtgtcctc gcgcatggcg gtgcccgggt agtcgtcgcg gaaaccgccc agacgcgcca
      301 gcatctcgcg gcggaaggac atgttggctc cgatgccgtg atccacgacc tccaggccgt
      361 cgcgcacggc ggcaaagccg gcggtcaggg tgccatcctc gcgaagccgt ccgatcggca
      421 ggtcgtactg gtcctcgccc ggctgcagat tgcgggtgcg ccccgcgacg gcatccggac
      481 gcaggtccga cgagaaggca tccagcaggc tctgccgcca cccttcggag accaccacat
      541 cgtcgtccag aaaggcgatg atttcgctgc ccgcatgcag cagcgcctcg ttgcgcgatc
      601 gggtcatcca gcccgccagg cctgggcggt gcaggtacag caccccagga tgagcgaccg
      661 ccgctgcgcc gtccacccgc gacgcgtcga cgacgaccac ggcggtgtcc ggcgcgttcc
      721 gctgcagcga cgccaggcag cgggcgagga actccgggcg ctcgtaggtg acgatgcaca
      781 cggtgagctg cgggtcggac atgcgcctca gcctagtggc gcgcatcgtc gcgataggct
      841 caccccgggg acgggaggcg gcatggccga gcgcagggtg acggtgatca tcccgacgaa
      901 ccgtggcggc gcgtatctgg acgccgcagt ggcgtcggtg cggtcgcaga cccggcccgc
      961 gcacgagatc ctgctggtgg acgacggctc gccggaaccg ggtctcgccg acgtcgccgc
     1021 acgtctcggg ctccgctatc tccggcagga cgcttcggga ctctccgtcg cccgcaacac
     1081 cggtgtcgcg ggcgccgaag gggagtggat cgccttcctc gacgacgacg acgtctggca
     1141 tccggagcgc ctcgaagagc agctgcgcgc ccttgacacc gagggcgatg tcgtcgccgt
     1201 cagcaccggc gggtggtaca tggatgccga gggggtgccg ttcggcaccg gatggggagc
     1261 gcccccggcc acgtcggccc agttcatcgc gtacgaggcg gtcccgccgc ggatcaccac
     1321 cctcctcatc cgccggagcg agtatctcgc ggtcggcgga tgccgccgtg cgatggagcc
     1381 cgcggaggac aacgacctga tccagcgcct gctgcagcgc ggtgagttcg cgagcgtcga
     1441 tcggcagctc gtgggctacc gccggcacac ggggaacatc acccagcgct cgctggcggg
     1501 gcgcgaggcg agccggatcg tcatcgacga cctgctgcgc gacgcccgcc accgccagga
     1561 cggagaggtc gtcgcgctgc tgaagcggca gcgcaggacg gcgcgacgca tctcggcgtc
     1621 gggcaacacg ggcgagctga tcgcagcgct gagggcgggg gagtccggct atgcgttcca
     1681 gctcgccatg tggggactgc ggcatgtgcc cttccattcg gtggccgccg tcgcgcgacg
     1741 ctggcgctcc cgagaggcgt ccggtcgatg acgtccgcgc gtgaggaggg cacggacggc
     1801 cgcgcagccg gccgggacgg cggcgcgcag accttcacac tgatcatccc gacgcaccgg
     1861 cgccccggat tcgtggtcca ggctgtggac agcgcgctcc ggcaggagcg tgcgttcgac
     1921 cggatcatcg tgatcccgga cgggctggac gatccggctc cggacgcgct ggcggggctt
     1981 cccgtcgacg tgcatccggt cgcccacggc ggtgtctccg cggcgcggaa cgcgggcctt
     2041 gcgctcgccg acacggactg ggtgtgcttc ctcgacgacg acgatctgct gcatccggcg
     2101 tatctcgccg acctcgaggc ggccgtcggc gcgcatccgg agacctcggc gttcaatgcg
     2161 ccgtactgga gcttcgcggc cgtcgcgggt ccgcacgagg agttctcggc cacctcgctc
     2221 gacgaatgcc tctcggcgag cggatcggcc gtcccgcgca acgacatgtc ctacctcgag
     2281 atcacgggcc gcagctacga cgcccttctc gagaagctcg cgggctcgat gagcacggcc
     2341 gcggtgagga ccgcagtgct gcgcgaggcc ggcggattcc cggtcgacct gatcgccgcc
     2401 gaggactgga ccatgtacgt caacgtcgcg cggctcacgg aatggcgggt gctcgacgag
     2461 cgccgcgcgt tcttccgcgc gcacggcgac aatgcgatga gcggccgatc gcgcgggaag
     2521 atgctcgccc cgctgcgtgc gatccgctcg ttctggcggt ccgggcagcc atcgcctccg
     2581 cacgcccccc tcgaggcgta ccggcgggcc taccgcaacg aggtcatgac cgcgctcgca
     2641 gggtgccgcc gcaacggcga cgccgaaggc cgtgaagagg cgctgcgcat cgcggccgag
     2701 atcctgccgc ggcggtggga ccggatcttc atccggatgc cgcgtcccgt gcgcagcgtc
     2761 atgtggcggc tggagcgagg atcccgcgcc tcctgaggcc gcgcggcctg cggactactg
     2821 cagcgatccg agctccacga gccgggcgaa ctcggcatcc gcggccctga cctgctcgaa
     2881 cgtgccggtg gcggcgacct caccccggcg caggaagacg agctggtcca catggcggac
     2941 cgtcgagagt cgatgcgcca ccacgatgat cgtcatcgag ccctgcagcc gctggagggt
     3001 ctgggcgatc tcgtgctcgg tcgcattgtc gagcgcggaa gtggcctcgt cgagcacgag
     3061 cacgttcggc cgtcggtaca gcgcgcgggc gagacccacg cgctgccgct ggccgccgga
     3121 gaggcgcgtg ccgcgatcac cgagcatcgt gtccaggccg tcgggcagct ccgccaccac
     3181 ctcgtcgagc agcgacatgc gcacggcctc ccgcacgcgg tcgtcgtcga tcgcactgcg
     3241 ttcgagtccg aaggcgacgt tctcggccag cgtggcgttc gagatgaaca cgtcctgcgg
     3301 gacgacaccg agcccggcat gccaggccgc gaggtcgtcg tcgatgatct gatctccgca
     3361 ccggatgtcg cccgcggtcg gggacagcag ccccagcagc aggtcgagca gcgtggactt
     3421 ccccgcgccg ctggctccga cgaacgccgt cgtgcggttc tcggggatgc gcacgctgac
     3481 gcggtcgagc acgttctgcg tcgaatcggg gaagtggaac gagacctcgt tcacgacgat
     3541 gtcgccggtg ggcggcgtcg gagcctgcgg ggtctcgtcg tgctggccct cggtgtcgag
     3601 ctcgtcgagt gcggagacca tgatccgcag cccggcgtcg cccgcgcggg tggtcgcgac
     3661 gttcgccgtg acgcgtgtga gtgcggggag caggcgcatg gatgccgcgg cgaagacgcc
     3721 cagcacgccg aggatctggt cctgtgcgcc ggtgaccgag aggatcacgg cgatgccggc
     3781 gatcgcgacg aggaaggcga tctcgagcag gtagcgcggg acgtcgccga ggatgccggc
     3841 ctgctgctgg gcgcgggcgc cacgcaggcg cgcggcgcgg aagcggttca cgaagagctc
     3901 tgagctcgcc gtcagccgcg tgtcgcggaa cccctcgatg cccggcatca gcgactgcca
     3961 cgcctgcagc gatgatgcgg cgacctcctc gccgaggttg acctggcgac ggcggagcag
     4021 acgctgcact ccgaagacga tcaccccgaa gaggaaaccg gcgaacaccg tcgccagcag
     4081 cgagctccac gcgagcacca ggatgatcgc ggtcatggtc accacgtcgg cgacgagact
     4141 cacctgcgcc agcagcacgc tcgcggcctg acctgtcgcg tcgttgatgt tgcggtagat
     4201 ctcgctcgac gcccgtttgc gatgcgagcc gtacggcgcg agcacgtaac gccgcatcag
     4261 ggcggccgcg gccagcgccg agactctggt ggtccgcccg agaagccacc accggaacat
     4321 cagcgcgccg atgctcttga tcaggaaggc gatcagcacc aggccggcga tgacggggag
     4381 cagcacgctc acgtccgtgg tcccgatcgt cgtcgagatc caggtcagga aggcgccgtc
     4441 ggtcttcccc gaggtgatca gctgcatgag ggggagcatc gacagcaccc cgaccatgtc
     4501 gaggccggcc agcacgagcg agaccagtac cgtcgtcgtg acccaccggg cgggtgccgc
     4561 gccggtgacc tccaggagca ggcgcagccg cgacatcctc gtctgacgcg tcatctgccc
     4621 actgtcccct cgacggtccc gatcgcctcc cggcgtcccg agaaatctag catcgcggct
     4681 cccggccgga gcctgaagga gggcgcggat ggatcggaat atcggtctct ttcgcatctg
     4741 gcccgattcg actatcatta gcggcaatgc ccgtcggttg agcggctcta tgggggagtc
     4801 gcgccggggc gataagcatt ggggaatgct ctcatgagcg agaacacgaa gaccgctggc
     4861 gacggcatca gtcgccgcac cgtcacgaag gcgatggcat ggtccgtgcc cgctatcgcc
     4921 gtcgccgcac cgatcccggc cttcgccgcg tcgggcaagc cgccgacagt cctgcccggc
     4981 aaggtgttca agtggcctgg cggcagctgc gtcaacacgc ccagcgggct ggacaacaac
     5041 aaggcgtacc tgttcacctt ccgtgtcacg aacacgtcga acaagccgat ctacatctac
     5101 agcgtcacca tcacgacgac cagcgggatc acgttcaaca cggtcggcat gacgcccggc
     5161 ctcggcacgc cgctggcccc gaaccagtcg accgacgtgt cggtgtgggc gaacagcagc
     5221 gacagcggca acctctcctt ccaggcgacg gcgaccatca cgtggggtca caactacccg
     5281 ggtcccgacc ccgacaacca cgcaccgatc gtgaagacct ggcaggtcaa cggcacgccc
     5341 acgaccacgt cgcaggggta cagccagtgc cagaacccgg gctacatcgg gctctgagcc
     5401 gcaccggctc gtgacatgct cacgggccag cacgcagtcc tcgaaccacc acgccgaccg
     5461 cagcggtcgg cgtggtggtt ctgcgttcgg gcgaagacac ggtgctgacg cccggtatgc
     5521 ggcgggacgc ggtcagcgca gcggaagggc ggcgcggaat gcggcgacgc gcgtcgacca
     5581 gtccgcggcg cgcgcgttcg caccctcagg gaaggcggat gccgcaggaa gggtggacgc
     5641 cactgctgag gcgaagtccc ctcccgacgc gatcgtgacc ccaggctcat cgcggaagcc
     5701 cgccaccggg gtggagacga tcgggcgccc gacggcctga tactcgtaca gcttgatcgg
     5761 gtcgaggctc tcggtgaacg cggtcacgag gtgcgggacg acgaggacat ccgcgtgctg
     5821 caggtagccg ggcacagctt cccggtcccg tgcgcccagc acgatcgcac ccgcggcgcg
     5881 gacacgctcg ccgtcggccg ccgaccaggc ttcgggcccg acgagcacca gatgaccttt
     5941 cccgcgcagc gcggtcgcgg tcgcctcgca caggtcgaca tcgatgcggt cgcggtgcag
     6001 ggtgccgagg tacagggcgt acggtccctc gggagcatcc gcggggcgcg gagcgggtga
     6061 gaggtagcgc gccgtgtcga cgccgttgcg caccagcacg atgtcgtcgc gctgcgagcg
     6121 cttgcggcgg atcagctcac gcgagcacgc gaccacggcg gcggcgtgct gcagcagcca
     6181 cttctcgccc tcggcgatgc gctgctgcgc cgactccggg cgttctgcgg cgagccagtc
     6241 gtcggtcatg tcgtacagca ccggccagcc cgtgcgctgc gcgagctgca ccgcggcggg
     6301 gtcgttgatc cagagcaccg gatgcggcat ccgcagcgtc tccacggccg cgcggacact
     6361 gctcgcgagc cggtcgtcgg caccgcgatc gatgcggcgg gggagccact tcacagggcg
     6421 cagcgtccac agccggtcgt cgatgcgcgt caggccgcgc cccgtgctcg gacgtcgcct
     6481 gtggcgcagg tcgtggatcg ggtcgtccgg cggctccacg aagagcacac ggagctcggg
     6541 gtcggcatcg agcagtcctg ccaccaggta ctggttgcgg cgccacacgc catcccaccg
     6601 ttcgagggag agcacgacga ggtcggtcat cgcagccgcc tctcgatcgc acgccggtag
     6661 acgtcgtcgg tgcgcgccga ctgctgcgcc agcgagaact ccgtgcgggc gcgctccccg
     6721 gcctgctctg cgagacgcat ccgtcgctcc tcatcctcgg cgagcgtgcg cagcgcgtcg
     6781 gcggcatccg ggatgtcccc cggacggtag ccgctgcgcg catccagccc ttgcagtacg
     6841 tcgaggtggc cggcggcgga tgccgcgacc ggcgccgcgc ccgcgcccat cgcctcgatg
     6901 agcgcgagtc ccaggccttc aacctcgcag ggcgcgagga acagggctgc ccgctccagg
     6961 cgtgcgggca gatcctcgcg gaaccccagc agccgcaccg cgtcggaga
//
LOCUS       DATMSX010000428         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_148011,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000428 DATMSX010000000
VERSION     DATMSX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148011"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VN041_13660"
     CDS             <1..944
                     /locus_tag="VN041_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HWU30123.1"
                     /translation="AWGEHVVAVGGTAPAVMAARPQVVEGTLSIGVHGPGFHVLFSRL
                     FNGLGSYRFGGSRTGGRELLAGTVTPNFWHAPTSNERGWNGPFDEGQWLLASRYGRAS
                     GYHEPADISVTQEQGGVRIGYRYELPTTPATHCELSYLIDGSGRVEVTQRMHVPDGMP
                     AMPEFATMLAMPADYDRVRWYGEGPEECYVDRRAGARLGVYDRRVPDMLTPYLNPQEA
                     GNRTGVRWAEVTDAQGVGLRFEGDPTMEFSALSWTPAEIESALHPNELPPIHRTIVRP
                     ALMRRGVAGDDSWGARPLAEHLLPTGDLEFRFAFRGI"
     gene            complement(978..2006)
                     /locus_tag="VN041_13665"
     CDS             complement(978..2006)
                     /locus_tag="VN041_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30124.1"
                     /translation="MAARADDARVWRTPSIIDVAREAGVSAQTVSRVANGMDIVRPAT
                     REKVLAAMDRLGYRPNSAARALKSGRFRTLGVIMSGLSSYGNMRTLEAIANEAARAGY
                     SLALTPLWTIAQAGFAGAFVRLAEQAVDGVLVVLEAHDLHDSDLAHIAGIPAVVVTAG
                     GSQPYPTVDIDQAQGARLATEHLLSLGHRTVWHISGPEDSFSARDRAASWEATLRAHD
                     REVPPVLHGDWSTASGFAAGRVLAADPSVTAVFAANDEMALGAMRALREAGRVVPDDV
                     SVVGFDDMDMAADFQPPLTTVHQYFDRVGTAAVEMLVDRIEQRGEPATGLVPTELVVR
                     ASTAPPRR"
     gene            complement(2029..2874)
                     /locus_tag="VN041_13670"
     CDS             complement(2029..2874)
                     /locus_tag="VN041_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU30125.1"
                     /translation="MRIALTGASGKLGAVVARELRAHGWDVIGMDVVGTRAPDFVQVD
                     LTDYGQVVDALTAVGDRHDGIDAVVHLGAVPAPGIRTDVATFHNNMPATFNVFWAAVR
                     LGIRRIVYASSETVLGLPFDVPPPYIPVDEEYPARPESVYSLVKTLEEQLARELARWH
                     PDLSITALRFSNVMAPEDYAAFPSFDADALQRKWNLWGYIDARDGAQAVERALQVAKP
                     GFDRFIIAAADTVMSRPNAELIAEVFPGVPVTREVGPNETLLSIDHARKVLGFDPQHS
                     WRDHV"
     gene            2949..3959
                     /locus_tag="VN041_13675"
     CDS             2949..3959
                     /locus_tag="VN041_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30126.1"
                     /translation="MAVTLHDVARLSGVSIKTVSNVINDYPHIRPTTREKVEKAIAEL
                     GYTPNLTARNLRSGRTGAIALALPDLSLAYFGELAAQVISEAEAAGVVVLVEQTGADR
                     DRELELLRSPRLKLTDGLIFSPLGMGQEDVAALDVPYPMVLLGERIFDGPTDHVTMRN
                     VEAARAATEYLLSLGRRRIAVVGAHEGEVIGSAALRMEGYRQALDAAGIRYDDDIIGY
                     TTLWHRANGADSMRELLARGAEFDAVFGLNDTLALGAMRVLQEAGRRVPDDVAVVGFD
                     GLDEAEYSIPSLTTVDPGRDWIAKTAVTTLLARIAGEADAAPQTLLSDFRILERESAP
                     AV"
     gene            4072..5385
                     /locus_tag="VN041_13680"
     CDS             4072..5385
                     /locus_tag="VN041_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU30127.1"
                     /translation="MKFRILAAVGIIALAGTAITGCAGASASGGDANELTFMFRGNDA
                     EKAAYTAAIKQFEKDNDANVKIIMTTADDYNTKLKAAITGHKVPDVFYIGPGDVQSYA
                     KSGVITDITKQVESAPNFSDIWDYGIDSYRYDGKVQGKGSIYAVPKDLGPFSFGYNKT
                     MFEKAGIPLPDNDKPYTWAEFAEVAKKLTQDTDGDGKLDHWGTGLNVTWNLQSWVWSN
                     GGSWLNDDQTKVTVDTPEFAEALQNFADLKSVSPSTTESASLDTYQRWMKGEIGFFPV
                     GPWDVPTYEKLDFDWDLIPYPVGATGEPATWIGTLGIAVSASTKNSELATKLALYLST
                     DKTAQQSLVDAGVQIPNLKSMADAWVTGSDLPPANKQEFLDIVKDYGRALPANNTYSG
                     EWYDEFWKNIQPVLDGKVTAADYVKKEQPAMQKLLDKAIQDAQLG"
     gene            5475..6383
                     /locus_tag="VN041_13685"
     CDS             5475..6383
                     /locus_tag="VN041_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30128.1"
                     /translation="MAALPHAARRSTLHRREHLTALAFIAVPVIGFLMFIAFPMLFAV
                     YVSFTKWNGLTAPRPNGIQNYIDIFSDPYFWQSMGNTVFMMIGIPIGLILSLLLALAL
                     NRRMRGTTFFRTVYYVPVISSLAAVAILWQLAYNGDVGLINHLLALVGIDGPNWLQDP
                     VWAKPAIVIMAIWKGLGFSMLLYLAALQSVPRHLYEAAAIDGAGAFRQFRYITVPMLR
                     PVTFFLVITSIIGGSQIFIEINIMTPTGGPEFSTASIVWYIWQKAFNYSQMGYATAMS
                     VVLGLLVFVVTAIQFQINRRSQFSIE"
     gene            6391..7329
                     /locus_tag="VN041_13690"
     CDS             6391..7329
                     /locus_tag="VN041_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30129.1"
                     /translation="MTIDAPDTVATVVSSPGGGRRLADTIGRPVRSAARTRSHRLTNV
                     ILTIVLSLGGIVMIAPLVWTLSTALKSKSAALTDPLQWIPDPIQWDNFVRVWDAGPLA
                     SGIANSLIVSVTVTVIGTGFSALAAFAFAKMRLPFKNAIFLSLLAAIMIPFPTIMIPQ
                     FIMFANLGLVDTLWPLILPGLFGNIVMVFFLRQFLDSVPDSIIEAAKLDGAGYLRIFT
                     TLIFPAIRPAVAAQFILWFMAVWNDYLAPVIYLNTPAKQTLQVVIATFNSTYATQRDY
                     PLIMAASFIALIPVLLVFVIFQKQIIESVALTGAKG"
     gene            7332..>7531
                     /locus_tag="VN041_13695"
     CDS             7332..>7531
                     /locus_tag="VN041_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HWU30130.1"
                     /translation="MHEPDTGSWGARNAHDPTIVRGDDGLWYMFSTDAAAGVDSIPAG
                     AHVRTSPDLVEWTFRGTALPGV"
BASE COUNT         1212 a         2649 c         2485 g         1185 t
ORIGIN      
        1 tggcctgggg tgagcacgtg gtcgcagtcg gcgggacggc gccggctgtc atggccgccc
       61 gcccccaggt cgtggagggc accctgagca tcggcgtgca tggcccgggg ttccacgtgc
      121 tcttctcgcg gctgttcaac gggctcggct cgtaccgctt cggcgggtcg cggacgggag
      181 ggcgcgagct gctggccggc accgtgaccc cgaacttctg gcacgcgccg acgtcgaacg
      241 agcgcggctg gaacgggccg ttcgacgaag ggcagtggct gctcgccagc cggtacgggc
      301 gggccagcgg ataccacgag ccggcggaca tctcggtgac gcaggagcag ggcggcgtgc
      361 gcatcggcta ccgctacgag ctgccgacga ccccggccac acactgcgaa ctgagctacc
      421 tgatcgacgg ctccggccgg gtggaggtca cacagcggat gcatgtgccg gacggcatgc
      481 ccgcgatgcc ggagttcgcg accatgctgg ccatgcccgc cgactacgac cgggtgcgct
      541 ggtacggcga ggggccggag gagtgctacg tcgatcgccg cgccggcgcc cggctcggcg
      601 tctacgaccg ccgagtgccg gacatgctca cgccctacct gaaccctcag gaggcgggga
      661 accgcacggg cgtgcgctgg gcggaggtga cggatgccca gggcgtcggg ctgcgcttcg
      721 agggtgatcc gaccatggag ttctcggcgc tgagctggac gccggccgag atcgagagcg
      781 cactgcatcc gaacgagctg ccgccgatcc accgcacgat cgtgcggccg gcgctcatgc
      841 gccgcggggt cgccggcgac gattcgtggg gtgcgcgtcc gctggccgag cacctcctgc
      901 cgaccggaga cctcgagttc cggttcgcct tccgggggat ctagcccgtc agtcccggtg
      961 tcgccccgcc cgtgccctca ccggcggggc ggcgcggtgc tggcgcgcac gacgagctcg
     1021 gtggggacca gccccgtcgc aggctccccg cgctgctcga tccggtcgac gagcatctcc
     1081 acggcggcgg tgccgacgcg atcgaagtac tggtggaccg tggtcagcgg cggctggaag
     1141 tcggcggcca tgtccatgtc gtcgaagccg accacgctca cgtcgtccgg cacgacgcgc
     1201 ccggcctcgc gcagggcacg catggcgccc agggccatct cgtcgttcgc ggcgaacacg
     1261 gcggtcacgg aagggtcggc ggccagcacg cgtcccgcgg caaagccgga tgccgtgctc
     1321 cagtcgccgt gcagcaccgg cgggacctcg cggtcgtgcg cgcgcagcgt cgcctcccag
     1381 gaggcagcgc ggtcacgagc ggagaacgag tcctcggggc cgctgatgtg ccacaccgtg
     1441 cgatggccga ggctgagaag atgctcggtg gccagccgcg cgccctgtgc ctggtcgatg
     1501 tcgacggtcg gatagggctg ggagccgcct gccgtcacca cgacggcggg gatgccggcg
     1561 atgtgcgcga gatcggagtc gtgcaggtcg tgcgcctcga gcacgacgag caccccgtcc
     1621 acggcctgct cggccagccg cacgaaggcc ccggcgaaac ccgcctgggc gatggtccac
     1681 agcggcgtca gcgccagcga gtagcccgcc ctcgccgcct cgttggcgat ggcctcgagg
     1741 gtgcgcatgt tgccgtagct ggacagtccc gacatgatca cgccgagggt gcggaaccgg
     1801 ccggacttca gggcgcgcgc ggcggagttg ggtcggtagc ccagccggtc catcgcggcc
     1861 agcaccttct cgcgcgtcgc cggccgcacg atgtccatgc cgttggccac ccgcgagacg
     1921 gtctgcgcgg acaccccggc ctcgcgcgcg acgtcgatga tcgacggggt gcgccatacg
     1981 cgtgcgtcgt ctgcccgagc tgccaccgat gctcccgttc tcgccgtctc agacgtggtc
     2041 gcgccaggag tgctgcgggt cgaagcccag caccttccgc gcgtgatcga tcgacagcag
     2101 cgtctcgttc gggccgactt cgcgggtcac cggcacgccg gggaagacct ccgcgatcaa
     2161 ctcggcgttg ggccgtgaca tcacggtgtc ggccgcggcg atgatgaagc ggtcgaaccc
     2221 gggcttcgcc acctgcagcg cccgctccac cgcctgcgcg ccgtcgcggg catcgatgta
     2281 gccccacagg ttccacttgc gctgcagcgc atcggcgtcg aacgacggga aggccgcata
     2341 gtcctcgggc gccatcacgt tcgagaagcg cagggcggtg atcgacaggt cgggatgcca
     2401 gcgcgccagc tccctggcca gctgctcctc gagcgtcttc accagcgagt acaccgattc
     2461 ggggcgcgcc gggtactcct cgtcgacggg aatgtagggc ggcggcacat cgaacggcag
     2521 gccaagcacc gtctcgctcg acgcgtagac gatgcggcgg atgccgagcc gcacggccgc
     2581 ccagaagacg ttgaacgtcg cgggcatgtt gttgtggaag gtcgcgacgt cggtgcggat
     2641 gccgggcgcg gggaccgcgc ccaggtgcac cacggcgtcg atgccgtcgt gccggtcgcc
     2701 gacggcggtg agcgcgtcca ccacctgccc gtagtcggtg aggtcgacct gcacgaagtc
     2761 gggggcgcgc gtgcccacga catccatgcc gatcacgtcc cagccgtgcg cgcgcagttc
     2821 gcgggcgacg acggcgccga gctttccgga tgccccggtg agtgcgatgc gcatgtgtcg
     2881 tccttccgtc ggatgcctgt tctgcaacgt tatatggtga ctgtgtccgg aacaggggga
     2941 gaagtcgcat ggctgtgacg ctgcatgatg tggcgaggct ctcgggcgtc tcgatcaaga
     3001 ccgtctcgaa cgtcatcaac gactacccgc acatccgccc caccacgcgc gagaaggtcg
     3061 agaaggccat cgccgagctc ggctacacgc cgaatctcac cgcccgcaac ctgcgctcgg
     3121 gtcggaccgg tgcgatcgcc ctcgccctgc ccgatctcag cctcgcgtac ttcggcgaac
     3181 tcgccgcgca ggtgatcagc gaggccgagg cggccggcgt cgtcgtgctg gtcgagcaga
     3241 ccggagcgga ccgcgaccgc gagctcgagc tgctgcgcag cccgcggctg aagctcaccg
     3301 acggactgat cttcagcccg ctgggcatgg gccaagagga cgtcgccgcg ctcgacgtgc
     3361 cctatccgat ggtgctgctg ggcgagcgca tcttcgacgg gcccaccgat cacgtcacca
     3421 tgcgcaatgt ggaggccgcg cgcgccgcga ccgagtacct gctctcgctc ggccgccgcc
     3481 gcatcgccgt ggtgggagcg cacgagggcg aggtgatcgg ctccgcggct ctccggatgg
     3541 agggctaccg gcaggcgctg gatgcggccg gcatccgcta cgacgacgac atcatcggct
     3601 acaccaccct ctggcaccgc gccaacggcg ccgattcgat gcgcgagctc ctcgcgcgcg
     3661 gcgccgagtt cgacgcggtc ttcgggttga acgacacgct cgcgctgggt gcgatgcggg
     3721 tgctgcagga ggccggacgg cgggtgcccg acgacgtcgc cgtggtgggc ttcgacggcc
     3781 tcgacgaggc cgagtactcg atcccgtcgc tgaccacggt cgacccgggc cgggactgga
     3841 tcgcgaagac cgcggtcacg acgctgctcg cgaggatcgc cggagaagcg gatgctgcgc
     3901 cgcagaccct gctgagcgac ttccgcatcc tcgagcgcga gtccgcgccc gcggtctgac
     3961 gcatccgccg gtaatcggca ttgacagctc gccctgttcg agtcacaatg catttacaac
     4021 gtttactaca acgttgtaaa gaacctcact ccgagaggaa gacccagcac aatgaagttc
     4081 cggatcctgg ccgccgtggg catcatcgcc ctcgccggca ccgcaatcac cggctgcgca
     4141 ggcgcgagcg cctcgggcgg cgacgccaac gagctcacct tcatgttccg cggcaacgac
     4201 gcggagaagg cggcgtacac cgctgcgatc aagcagttcg agaaggacaa cgacgcgaac
     4261 gtcaagatca tcatgaccac cgccgacgac tacaacacca agctcaaggc cgcgatcacc
     4321 gggcacaagg tgcccgacgt gttctacatc ggcccgggcg acgtgcagtc ctacgccaag
     4381 tcgggcgtca tcaccgacat caccaagcag gtcgagagcg cgccgaactt cagcgacatc
     4441 tgggactacg gcatcgacag ctaccgctac gacggcaagg tgcagggcaa gggctcgatc
     4501 tacgccgtgc ccaaggacct cggcccgttc tcgttcggct acaacaagac gatgttcgag
     4561 aaggcgggca tcccactgcc cgacaacgac aagccctaca cctgggccga gttcgccgag
     4621 gtcgcgaaga agctgaccca ggacacagac ggcgacggca agctcgacca ctggggcacc
     4681 ggcctgaacg tcacctggaa cctgcagtcg tgggtgtggt cgaacggcgg atcctggctg
     4741 aacgacgatc agacgaaggt caccgtcgac accccggagt tcgccgaggc gctgcagaac
     4801 ttcgcggacc tgaagtcggt cagcccgtcc acgaccgagt cggcgagcct ggacacctat
     4861 cagcggtgga tgaagggcga gatcggcttc ttcccggtgg gaccgtggga cgtgcccacc
     4921 tacgagaagc tcgacttcga ctgggatctg atcccctacc cggtcggtgc gacgggtgag
     4981 ccggccacct ggatcggaac gctcggcatc gccgtgagcg cgagcacgaa gaactccgag
     5041 ctcgccacga agctcgcgct gtacctcagc acggacaaga ccgcgcagca gtcgctcgtg
     5101 gacgcgggtg tgcagatccc gaacctgaag agcatggccg atgcctgggt gaccggctcc
     5161 gacctgccgc cggccaacaa gcaggagttc ctggacatcg tcaaggacta cggccgcgcc
     5221 ctgcccgcca acaacaccta cagcggggag tggtacgacg agttctggaa gaacatccag
     5281 ccggtgctcg acggcaaggt cacggccgct gactacgtga agaaggagca gcctgcgatg
     5341 cagaagctgc tcgacaaggc catccaggac gcccagctgg gttgatccgt cccgccgggg
     5401 gcgtccgcga cggacgcccc cggctcctct ctccggccca cggccggacc gcatctcacc
     5461 ctgggaggtg gatcgtggca gcactgccgc acgccgcgcg tcgttccacg ctgcatcgcc
     5521 gggagcacct gaccgcgctc gccttcatcg ccgtgccggt catcggcttc ctcatgttca
     5581 tcgcgttccc gatgctcttc gccgtgtacg tctccttcac gaagtggaac ggcctcaccg
     5641 caccccggcc caacggcatc cagaactaca tcgacatctt ctccgacccg tatttctggc
     5701 agtcgatggg caacaccgtc ttcatgatga tcggcatccc gatcgggctg atcctctcgc
     5761 tgctgctcgc cctcgcgctg aaccgcagga tgcgcggcac caccttcttc cgcacggtct
     5821 actacgtgcc ggtgatctcg tccctcgcgg cggtcgccat cctgtggcag ctggcgtaca
     5881 acggcgacgt cggactgatc aaccacctgc tcgcgctggt cggcatcgat ggaccgaact
     5941 ggctgcagga tccggtctgg gccaagcccg cgatcgtcat catggccatc tggaagggcc
     6001 tcggcttctc gatgctgctc tacctggcgg cgctgcagtc cgtgccccgt catctttacg
     6061 aggccgccgc gatcgacggc gccggcgcat tccgccagtt ccggtacatc accgtcccga
     6121 tgctgcggcc cgtgaccttc ttcctggtca tcacgagcat catcggcgga tcccagatct
     6181 tcatcgagat caacatcatg accccgaccg gcggccccga gttctccacc gcctccatcg
     6241 tctggtacat ctggcagaag gcgttcaact actcgcagat gggctatgcg acggcgatgt
     6301 cggtcgtgct ggggctcctg gtcttcgtcg tcacggcgat ccagttccag atcaaccgtc
     6361 gttcccagtt ctccatcgag tgaggcaacc atgaccatcg acgcacctga caccgtggcc
     6421 accgtcgtct cctccccggg aggtgggcga cgcctcgccg acaccatcgg ccgacccgtc
     6481 cgctccgccg cgcgcacgcg cagtcaccgc ctgacgaacg tgatcctgac gatcgtgctc
     6541 tccctcggcg ggatcgtgat gatcgcgccg ctcgtgtgga ccctcagcac tgcactgaaa
     6601 tccaagtcgg ccgcactgac cgacccgctg cagtggatcc ccgacccgat ccagtgggac
     6661 aacttcgtgc gggtgtggga tgccggcccg ctcgcctcgg gcatcgcgaa cagcctcatc
     6721 gtctcggtga ccgtgaccgt catcggcacc gggttctcgg cgctcgccgc cttcgcgttc
     6781 gcgaagatgc ggctcccctt caagaacgcg atcttcctct cgctgctcgc cgcgatcatg
     6841 atcccgttcc cgacgatcat gatcccgcag ttcatcatgt tcgcgaatct cggcctggtg
     6901 gacaccctgt ggccgctgat cctcccgggg ctgttcggga acatcgtgat ggtcttcttc
     6961 ctgcggcagt tcctcgactc cgtgccggac tcgatcatcg aggcggccaa gctcgacggc
     7021 gccggatacc tgcgcatctt cacgacgctg atcttccccg cgatccgtcc cgccgtcgcc
     7081 gcgcagttca tcctctggtt catggccgtg tggaacgact acctcgcacc ggtcatctac
     7141 ctgaacaccc cggcgaagca gaccctgcag gtcgtgatcg caaccttcaa ctcgacctat
     7201 gcgacgcagc gcgactaccc gctcatcatg gcggcatcgt tcatcgcgct gatcccggtg
     7261 ctgctggtct tcgtgatctt ccagaagcag atcatcgagt ccgtcgcgct gaccggggcg
     7321 aagggctgat cgtgcacgaa cccgacaccg gctcctgggg agcccgcaac gcgcacgacc
     7381 ccacgatcgt ccgcggcgac gacgggctct ggtacatgtt ctcgaccgac gccgccgccg
     7441 gcgtcgacag catcccggcg ggtgcgcacg tgcgcacctc gcccgacctg gtggagtgga
     7501 cgttccgcgg caccgccctg cccggtgtgc a
//
LOCUS       DATMSX010000429         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_25164,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000429 DATMSX010000000
VERSION     DATMSX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25164"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1514
                     /locus_tag="VN041_13700"
     CDS             <1..>1514
                     /locus_tag="VN041_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWU30131.1"
                     /translation="YFVATLLLRRQGAGPTVLVSPLLALMRDQIAAAERAGVRAVAIN
                     STNAHEWDDVLARLDRDEVDVLLVSPERLNNPAFREQQLPTLVQRIGMLVVDEAHCIS
                     DWGHDFRPDYRRLRDLIAQMPDGVPVLATTATANSRVVADVAEQLGGEEVLTIRGPLA
                     RASLRLGVLQLPNAASRLAWLLSHLDDLPGSGIIYTLTVAAAVDTARLLREHGHDVRA
                     YTGQTDAEEREESEGMLKRNEVKALIATSALGMGFDKPDLGFVIHLGAPSSPVAYYQQ
                     VGRAGRASDSADVLLLPGVEDRDIWHYFATASMPDRERAERVIAALGEVPISTPALEA
                     MVDIRRTPLELLLKVLDVDGAVRRVQGGWISTGAGWTYDAERYERIAAERRAEQQHMI
                     EYQQTGGCRMEFLQRSLDDATAAPCGRCDNCAGAWFPTEIGADAGAAASGALDRVGVP
                     IEPRRQWPTGADRLGVPVKGRIGSDEQAAEGRALARLTDLGWGGTLRELFAAGA"
BASE COUNT          222 a          518 c          554 g          220 t
ORIGIN      
        1 gtacttcgtc gcgaccctgt tgctgcgcag gcagggggcg gggcccaccg tgctggtctc
       61 gccgctgctc gcgctgatgc gtgaccagat cgccgccgcc gaacgcgccg gggtgcgggc
      121 ggtcgcgatc aactccacca atgcgcacga atgggacgac gtgctggcgc ggctcgaccg
      181 cgatgaggtg gacgtgctgc tggtctcacc tgagcgactg aacaatcccg cgttccgtga
      241 gcagcagctg ccgacgctgg tgcagcggat cgggatgctc gtcgtagacg aggcgcactg
      301 catcagcgac tggggtcatg acttccgtcc cgactaccgc cggctgcgcg acctgatcgc
      361 gcagatgccg gacggggttc cggtgctcgc caccacggcc acggcgaaca gtcgcgtggt
      421 ggccgacgtc gccgagcaac tgggtggcga ggaggtcttg acgatccgtg gaccactcgc
      481 ccgggcatcc ctgcgactcg gcgtgctgca gctgccgaac gccgcgagcc ggctggcctg
      541 gctgctgagt cacctcgacg atctgcctgg ttccggcatc atctacacgc tcacggtcgc
      601 ggcggccgtc gacaccgcgc ggctgctgcg cgagcacggt catgacgtgc gcgcgtacac
      661 cgggcagacg gatgccgagg agcgcgagga gtccgagggg atgctcaagc gcaacgaggt
      721 gaaggcgctg atcgccacca gcgcgctggg gatgggcttc gacaagcccg atctcggatt
      781 cgtgatccac ctgggcgccc cgtcgtcgcc ggtcgcgtac taccagcagg tcggacgcgc
      841 gggacgcgcc agcgacagcg ccgacgtgct gctgctcccc ggggtggagg acagggacat
      901 ctggcactat ttcgccacgg catccatgcc cgacagggag cgtgccgagc gggtgatcgc
      961 cgcgctcggc gaggtcccga tctcgacacc ggccctcgag gccatggtcg acatccgccg
     1021 caccccgctg gaactgctgc tcaaggtgct cgacgtcgac ggggcggtcc ggcgcgtgca
     1081 gggcggctgg atatccaccg gggccggctg gacctacgac gccgagcgct acgagcgcat
     1141 cgccgccgag cggcgcgccg agcagcagca catgatcgag taccagcaga ccggcggatg
     1201 ccgcatggag ttcctgcagc gctccctgga cgatgcgacc gctgctccct gcggccgctg
     1261 cgacaactgc gccggtgcct ggttcccgac cgagatcgga gcggatgccg gggccgccgc
     1321 atccggtgcc ctggaccgtg tcggcgtgcc catcgagccg cgtcgtcagt ggcccaccgg
     1381 tgccgaccgg ctcggcgtcc ccgtgaaggg gcgcatcggc tcggatgagc aggccgctga
     1441 aggccgtgcg ctggcacggc tgacggatct gggctggggc ggcacgctgc gagagctgtt
     1501 cgccgcggga gcgg
//
LOCUS       DATMSX010000430         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_53810,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000430 DATMSX010000000
VERSION     DATMSX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53810"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VN041_13705"
     CDS             complement(<1..307)
                     /locus_tag="VN041_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30132.1"
                     /translation="MDNLPRRGLAIGILLFAAFMDLLDVTIVQVALPAIGRDLDAGSG
                     ALEWIVSGYMLAFAAALVTGGRLGDIFGRRRVFLTGIAGFTLVSALAAAAVSAEMLVT
                     "
     gene            403..864
                     /locus_tag="VN041_13710"
     CDS             403..864
                     /locus_tag="VN041_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU30133.1"
                     /translation="MSSDPELTRGLLDRIQRFIGSAVLTNERIARSLGINVVDLQALG
                     MIARAGDRLTTGEVSQLTELPTSTTTRVLDRLERNGLIVRDADPADRRRVVVRISDHA
                     AHLLGRGGDDDPYAPILERMGRVNALFTPEELETVVRYFDAMEAEAAGDSA"
     gene            complement(878..1321)
                     /locus_tag="VN041_13715"
     CDS             complement(878..1321)
                     /locus_tag="VN041_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWU30134.1"
                     /translation="MSELRAHKILQPMGPAGAIVLDDDEVAILSTAKAFPVVVAIGGR
                     TARLRLARMGGQNLIGFSKAVRTEMGVELGDEFDVVIAADAAERTVDMPDALAEALAS
                     DPAAKTAFDALSYTRRKEIARGIAEAKQDATRERRLAKALEELRG"
     gene            1418..2452
                     /locus_tag="VN041_13720"
     CDS             1418..2452
                     /locus_tag="VN041_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWU30135.1"
                     /translation="MSDVTIFAPSPILTVTVENSGDADEIHIHAGGQGVWQARMLRRL
                     GVSVTMCCSLAGEAGQVIRHLLEDDGMTVVGIERPGRASAYVQDRRGGERAEIGTEVG
                     DPIARHELDELYGAVIREGMRSGVVILSGPAGDDVLPSDTYRRLAADLREAGCTVVVD
                     LAGERLDAALEGGVDLLKVSDEEMRAHGLDDDSPDALAAAMHSLRSRRAGTVIVTRAS
                     EPLLLLDEQGLLEVAPPRLEVVDSAGAGDSLTAGVVAGMVRGEPPRDAITLGAAAGAL
                     NVTRHGLGTGDADAIAELRGRIRVSVPGEDDDGGSMGVSPDGLAGLIETPRREAPGAE
                     TPRTESEGER"
     gene            2449..3207
                     /locus_tag="VN041_13725"
     CDS             2449..3207
                     /locus_tag="VN041_13725"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWU30136.1"
                     /translation="MTRVLVTNDDGIDSPGLHALAVVLRAHGMDVTIAAPVTQSSGSS
                     ASIMAENAEGQIRVERRTLEGLEGVPAFAVHGGPGLIALIAARGAFGDPTDVVASGIN
                     HGANVGRAILHSGTVGAALTGGLNGARGLAISLDVGLAPKTFRWEEAAEAAAELVPAL
                     LRHPRGTVINVNVPNTVESRGMVEADLAPFGIVQTTLTQQDEHHVRLAVEDLPREPVP
                     GSDAALLADGWITVTGLDPVSHVPLGIVAEVGAG"
     gene            complement(3214..3660)
                     /locus_tag="VN041_13730"
     CDS             complement(3214..3660)
                     /locus_tag="VN041_13730"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWU30137.1"
                     /translation="MASFVLERIITAPPEEVFDASLDVGLHVTSQSSHGERTVAGVTT
                     GMMGEGDEVTWSARHFGIRFRMSSVVFDVDRPRRFRDRQTRGPFAQFRHTHEFLPAEG
                     GTLMRDTLEFRSPFGPLGRLVDALVMRRHLIAVITERNDAITAHFG"
     gene            complement(3660..>3840)
                     /locus_tag="VN041_13735"
     CDS             complement(3660..>3840)
                     /locus_tag="VN041_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU30138.1"
                     /translation="SLTETGRDLVERATRALNTDVFRSPGIDADDASALVGIVARMRK
                     NAGDFTDPRPVPDPL"
BASE COUNT          584 a         1301 c         1419 g          536 t
ORIGIN      
        1 gcgtgacgag catctccgcg gagacggcgg cggcggccag cgccgacacg agcgtgaagc
       61 ccgcgatgcc ggtcaggaac acgcggcggc gtccgaagat gtcgcccagg cggccgcccg
      121 tcacgagcgc cgcggcgaac gcgagcatgt atcccgacac gatccactcc agtgcacccg
      181 agccggcgtc gaggtcgcgc ccgatcgccg gcagagccac ctgcacgatg gtcacgtcga
      241 gcaggtccat gaacgcggcg aacagcagga tgccgatggc gaggccgcgg cgggggagat
      301 tgtccatcgt ggggtccctt cctagaatga ggtgtctcca tctcggaaca ttcccgaagt
      361 gggattatcc cgatatggga atagtaggaa ggcgccctga ggatgtcaag cgaccccgaa
      421 ctgacccgtg gactgctcga ccggatccag cggttcatcg gaagtgccgt gctcaccaac
      481 gagcgcatcg cccgatcgct gggcatcaac gtggtcgacc tgcaggcgct cggcatgatc
      541 gcgcgtgccg gtgatcggct caccacgggg gaggtcagtc agctcaccga gctgccgacc
      601 agcaccacca ctcgcgtgct ggaccggctc gagcgcaacg gcctcatcgt gcgggacgcc
      661 gaccccgcgg accgcagacg cgtggtcgtg cgcatctcgg atcacgccgc tcacctgctg
      721 ggccgcggag gcgacgacga tccgtacgcg ccgatcctcg agcgcatggg gcgcgtgaat
      781 gcgctcttca cgccggagga gctcgagacc gtcgtccggt acttcgatgc gatggaggcc
      841 gaggccgccg gcgactccgc ctgagcgcag acggcgatca gccgcgaagc tcctccagag
      901 ccttcgccag gcggcgttcg cgggtggcat cctgcttggc ctcggcgatg ccgcgggcga
      961 tctccttgcg tcgcgtgtag gagagcgcat cgaaggcggt cttcgcggcc ggatcggatg
     1021 ccagcgcctc ggccagggca tccggcatgt cgaccgtgcg ctcggccgca tcggcggcga
     1081 tgacgacgtc gaactcgtct cccagctcca cgcccatctc ggtgcggacc gccttgctga
     1141 acccgatcag gttctgcccg cccatgcgcg ccagacgcag ccgcgccgtg cggccgccga
     1201 tcgcgacgac gacggggaag gccttggctg tgctgaggat cgccacctca tcgtcgtcga
     1261 gcacgatcgc cccggccggt cccatcggct gcaggatctt gtgcgctctc agctcgctca
     1321 tgccggccat cctacgtgcc ggcgggatct ggatcgacgg cttccgtcga acgcaagggg
     1381 gtgccgcacc cgcatccgcc cgggtaactt caccggcatg agcgacgtga cgatcttcgc
     1441 cccctcgccg atcctcacgg tgacggtcga gaactcgggc gatgccgatg agatccatat
     1501 ccacgcgggc gggcagggcg tgtggcaggc gcggatgctg cggcgcctcg gcgtctcggt
     1561 aacgatgtgc tgctcgctgg cgggggaggc cggccaggtc atccggcacc tgctcgagga
     1621 cgacgggatg acggtggtcg gcattgagcg tcctggcagg gcatccgcct acgtgcagga
     1681 ccgacgcggc ggggagcgcg cggagatcgg caccgaggtc ggcgatccga tcgcccggca
     1741 tgagctcgac gagttgtacg gggccgtcat ccgcgagggg atgcgctccg gcgtcgtgat
     1801 cctcagcgga ccggcaggcg acgacgtgct gccgtcggac acctaccgcc ggctcgccgc
     1861 ggatctgcgc gaagccggct gcacggtggt ggtcgacctc gccggcgagc ggctggatgc
     1921 cgcgctggag ggcggtgtcg acctgctgaa ggtcagcgac gaggagatgc gcgcgcacgg
     1981 tctcgacgac gactcgcccg acgcactcgc cgcggccatg cactcgctgc gatcgcgcag
     2041 agcgggaacc gtcatcgtca cgcgggcctc cgaaccgctg ctgctcctcg acgagcaggg
     2101 cctgctggag gtcgctccgc cgcgcctcga ggtggtcgac agcgccggcg ccggcgattc
     2161 cctcaccgcg ggcgtggtcg cggggatggt gcgtggcgaa ccgccgcgtg acgcgatcac
     2221 gctgggggcg gcggcgggcg cgctgaacgt cacgaggcac ggcctgggaa ccggcgacgc
     2281 ggatgccatc gccgagctgc gcgggcggat ccgggtctcg gtcccgggtg aggatgacga
     2341 cggcggcagc atgggtgtga gcccggacgg cctggccggc ctgatcgaga cgccccgccg
     2401 cgaggcaccg ggcgccgaga cgccccgcac cgagagcgag ggggagcgat gacccgcgtg
     2461 ctggtcacga acgacgacgg gatcgactct ccgggtctgc atgcgctggc ggtcgtcctg
     2521 cgggcgcacg gcatggatgt cacgatcgcc gcgccggtca cgcagtcgag cggatcgagc
     2581 gcgtcgatca tggccgagaa cgccgagggc cagatccgcg tggagcgacg cacactggag
     2641 ggcctggaag gcgtgccggc gttcgcggtg cacggcggtc cgggactgat cgcactgatc
     2701 gccgcccgcg gcgccttcgg cgatccgacg gacgtggtcg cctcgggcat caatcacggc
     2761 gccaacgtcg ggcgggcgat cctgcactcc ggaaccgtcg gcgcagccct gaccggaggc
     2821 ctcaacggcg cccgcggtct ggcgatctcg ctggacgtcg ggctggcgcc gaagacgttc
     2881 cggtgggagg aggccgccga ggccgccgcc gaactcgtgc cggcgctgct gcggcatccg
     2941 cgcggcacgg tcatcaacgt gaacgtcccc aacacggtcg agtcgcgcgg aatggtcgag
     3001 gccgacctcg ccccgttcgg aatcgtgcag acaacgctga cgcagcagga cgagcaccac
     3061 gtgcgcctgg cggtcgagga tctgcctcgc gaacccgtac ccgggagcga tgccgccctc
     3121 ctcgccgacg ggtggatcac cgtgacgggc ctcgacccgg tctcgcacgt gccgctgggc
     3181 atcgtggcgg aggtcggcgc aggatgagga tgctcagccg aaatgcgcgg tgatcgcgtc
     3241 gttgcgctcg gtgatcacgg cgatcagatg gcggcgcatg accagtgcgt ccaccagccg
     3301 gccgagcgga ccgaacgggg agcggaactc gagggtgtcg cgcatgagcg tgccgccctc
     3361 ggcgggcagg aactcgtggg tgtgccggaa ctgagcgaac ggacccctcg tctgccggtc
     3421 gcggaacctg cgcggtcggt cgacgtcgaa gaccaccgac gacatccgga agcggatgcc
     3481 gaagtgccgg gcgctccagg tcacctcatc gccctcgccc atcatcccgg tggtgacgcc
     3541 ggcgaccgtg cgctcgccgt gcgagctctg ggacgtcacg tgcaatccga cgtcgagcga
     3601 ggcgtcgaac acctcctcgg gcggcgcggt gatgatgcgc tccaggacga aggaggccat
     3661 cagagcgggt cggggaccgg gcgggggtcg gtgaagtcgc cggcgttctt gcgcatccgg
     3721 gccacgatgc cgaccagggc gctcgcatcg tcggcgtcga tgccggggct gcggaacacg
     3781 tcggtgttca gggcgcgggt ggcgcgctcg acgaggtcgc ggccggtctc ggtgagcgac
//
LOCUS       DATMSX010000431         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72876,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000431 DATMSX010000000
VERSION     DATMSX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72876"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VN041_13740"
     CDS             complement(<1..187)
                     /locus_tag="VN041_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HWU30139.1"
                     /translation="MQLLRLGAPGHEHPFVRDDEGVLRDLGPLTADIDGAFLAADGIA
                     RVREALTRGTLPGADGEG"
     gene            complement(206..1186)
                     /locus_tag="VN041_13745"
     CDS             complement(206..1186)
                     /locus_tag="VN041_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HWU30140.1"
                     /translation="MTGSHVLITDCDLPGDAAEQTLRAAGLRAARAPDTDADTLAALG
                     AEAEALIVQWHRIDGPLMDRMPRLKMISRLGIGYDMIDVAAATERGIAVANTPSYCIE
                     EVASHTIAMIMTQARGLPAYDTALRAGEWKPVAARPMAVRPSRTTVSVLGFGRIGSLV
                     ARGCRALGFRVLVADPYAPSEAIVAAGCEPVSIEQAIAAADLLTLHVPLTDATRHLID
                     ADSIATMKPGAVIVNTCRGPLIDEAALVAALHDGRVGAAALDVYEVEPLAPGAALREA
                     PNVLLTPHAAWYSPQALEDLPVHAAENIVRFLAGEPVGALVNPDHATARA"
     gene            1264..2013
                     /locus_tag="VN041_13750"
     CDS             1264..2013
                     /locus_tag="VN041_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30141.1"
                     /translation="MTVRRALITGASSGIGTATALELAGTGAAVWITYTGRETEAARV
                     AEECRAAGAPEARVSRLDLRDRESVDALITEITAAWGSLHVLVNNGGVCPYTAYDEID
                     IDEWDLVLETNARGTFLLTRAALPLLRAAEGDRSVINLSSIAGQVGALQTGIHYAASK
                     GALLAITRSFARHLAAEGIRVNAVTPGPVASAITDELSAEGREKLESGIPLGAFGRPA
                     DVAWIIVSLASERAGFITGATYDVNGGVRID"
     gene            2006..2347
                     /locus_tag="VN041_13755"
     CDS             2006..2347
                     /locus_tag="VN041_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU30142.1"
                     /translation="MTDPTRSAQDTVQAQLDAFNAHDLEAFVATYADDAVITGVATEP
                     IVGAAAIRAFYEPRLKNPELSCVIDTSVLFGTRWVVAQEQVINAGIATETIATFDVVD
                     GRIARASMLKA"
     gene            complement(2408..>2607)
                     /locus_tag="VN041_13760"
     CDS             complement(2408..>2607)
                     /locus_tag="VN041_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30143.1"
                     /translation="EERALAEGPPMGRIARSEEIAAVAAFLLSDEAPYLTGQSIAVDG
                     GWTSSFGDVSVPAALRERFAR"
BASE COUNT          397 a          927 c          920 g          363 t
ORIGIN      
        1 gaccctcgcc gtcggcgccg ggcagcgttc ccctcgtgag cgcctcgcgc acgcgggcga
       61 tgccgtccgc agcgaggaac gcgccgtcga tgtcggcggt gagagggccc aggtcgcgca
      121 gcactccctc gtcgtcgcgg acgaacggat gctcgtggcc gggggctccg agacgcagaa
      181 gttgcacggg tgctcctttc tcggttcagg ctcgcgccgt cgcgtggtcc ggattgacga
      241 gcgcaccgac cggttcgccg gccaggaacc ggacgatgtt ctcggccgcg tgcacgggaa
      301 ggtcctcgag cgcctgcggc gaataccagg cggcgtgcgg ggtcaggagc acgttcggcg
      361 cctcccgcag cgcggcgccg ggtgcgaggg gttccacttc gtagacgtcc agcgcggccg
      421 ctccgacgcg tccgtcgtgc agcgcggcga cgagggccgc ctcgtcgatg agcggaccgc
      481 ggcaggtgtt gacgatcacg gcgcccggct tcatcgtggc gatcgagtcg gcgtcgatga
      541 gatgacgggt ggcatccgtg agcggcacgt gcagggtgag caggtcggcg gcggcgatgg
      601 cctgctcgat cgagaccggc tcgcagccgg ccgcgacgat cgcctccgac ggggcgtagg
      661 ggtccgccac cagcacgcgg aagccgaggg cacggcatcc gcgggcgacg agcgagccga
      721 tgcggccgaa cccgagcact gacaccgtgg tgcgggaggg acgcaccgcc atcggccggg
      781 ccgccaccgg cttccactcg cctgcgcgca gcgcggtgtc gtaggccggg aggccacgcg
      841 cctgggtcat gatcatggcg atcgtgtgcg aggcgacctc ctcgatgcag tagctcgggg
      901 tgttcgcgac ggcgatcccc cgctcggtcg ccgcggcgac atcgatcatg tcgtacccga
      961 ttcccagccg gctgatcatc ttgaggcgag gcatgcgatc catgaggggt ccgtcgatgc
     1021 ggtgccactg cacgatgagc gcttcggcct cggcgcccag agcagcgagg gtgtcggcat
     1081 cggtgtcggg ggcccgagcg gcgcgcaggc ccgcagcgcg aagcgtctgc tccgctgcgt
     1141 cgccgggcag atcgcagtcg gtgatgagga cgtgggagcc agtcatgaac aaggatgcta
     1201 tagcatctag caaatgcctg tccagaggtt ccgctttgcc cgaatctgct atagcgtttt
     1261 gctatgactg ttcgccgcgc tctcatcacc ggggccagtt ccggcatcgg aacggccacc
     1321 gccctggaac tcgccggcac cggcgccgcc gtgtggatca cgtacacagg acgtgagacg
     1381 gaggccgccc gggtggcgga ggaatgccgt gccgccggcg cccccgaggc gcgcgtgtcc
     1441 cggctcgacc tgcgcgaccg cgaatccgtc gacgccctga tcacggagat caccgcggcc
     1501 tggggctccc tgcacgtgct cgtcaacaac ggcggggtct gcccttacac ggcctacgac
     1561 gagatcgaca tcgacgagtg ggatctggtc ctcgagacca acgcgcgcgg cacgttcctg
     1621 ctcacccggg ccgcgctccc gctgctgcgc gccgccgagg gcgacagatc ggtgatcaac
     1681 ctctcgtcga tcgccggcca ggtcggcgcc ctgcagaccg gcatccacta cgccgcgagc
     1741 aagggagcac tgctggccat cacccgcagc ttcgcccgcc acctcgcggc agaagggatc
     1801 cgcgtcaacg ccgtgacgcc gggaccggtc gcgagcgcca tcaccgacga actgtccgcc
     1861 gaagggcggg agaagctgga atccggcatc ccgctcggcg ccttcggccg ccccgccgac
     1921 gtcgcgtgga tcatcgtgtc actggcatcc gagcgcgccg ggttcatcac gggcgccacc
     1981 tacgacgtca acggaggagt acgcattgac tgacccgacc cgcagcgcgc aggacaccgt
     2041 ccaggcgcag ctcgacgcct tcaacgccca cgacctcgag gcattcgtcg cgacctacgc
     2101 cgacgacgcc gtgatcaccg gcgtcgcgac ggagccgatc gtcggagccg cagcgatccg
     2161 cgcgttctac gaaccccggc tgaagaatcc ggagctctcc tgcgtgatcg acaccagcgt
     2221 gctcttcgga acccgatggg tcgtcgcgca ggagcaggtg atcaacgccg gcatcgccac
     2281 ggagacgatc gcgaccttcg acgtggtcga cggccgcatc gcccgcgcct cgatgctgaa
     2341 ggcctagcgc gcctgcggca cccggcggac tgcgccgccg gcgcggcacc cgccgccccg
     2401 ggcacggtca gcgggcgaag cgctccctca gggcggcggg caccgacacg tctccgaacg
     2461 acgaggtcca tccgccgtcg accgcgatcg actgcccggt gaggtacggc gcctcgtcgg
     2521 agagcaggaa cgccgcgacc gcggcgatct cctcggaccg ggcgatacga cccatcggcg
     2581 gtccctcggc gagtgcgcgc tcctccg
//
LOCUS       DATMSX010000432         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_92001,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000432 DATMSX010000000
VERSION     DATMSX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92001"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1351)
                     /gene="hisD"
                     /locus_tag="VN041_13765"
     CDS             complement(14..1351)
                     /gene="hisD"
                     /locus_tag="VN041_13765"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWU30144.1"
                     /translation="MALHLKSAPTKSFADTAQHDVAEAVSAVIADIRAEGDTAVRRYA
                     EKFDRWSRDSYRLDDEEIERIIGTLDPQVIADIEFVQAQVRRFAQAQRDTLIDIEVET
                     LPGVLLGQKHVPVQAAGAYIPGGKYPLTASAHMTIITAKVAGVPRVVACTPPIRGEIP
                     AATVAAMKMAGADEIYILGGIQAVAAMAVGTETIAPVNMIAGPGNPYVAEAKRQLFGE
                     VGIDLFAGPTEILIVADETADPFFTAVDLLSQAEHGPESPAVLVTTSRELADRVMELI
                     DRILPEMPTKDYAEAAWRDWGQVIVADDLDEAYRIADSFASEHVQIFTAEPREALTKM
                     HDYGALFLGENTCVSYGDKVIGTNHVLPTLGAARYTGGLWVGKYLRTVTYQEIVDTAS
                     SAQLGDVLGRAARVELFEGHARSGDARVWRHEGKDFDWIDAALATADTAQATS"
     gene            1451..>1981
                     /locus_tag="VN041_13770"
     CDS             1451..>1981
                     /locus_tag="VN041_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30145.1"
                     /translation="MVVTSRDVARLAGVSQPTVSRALRDDARVSEDTKKRVREAAQLL
                     GYVPSEAGRALSSGRTRRIGLLVTDLDNQFYSHIIAPVHRELERLGYQLMLHTETADN
                     ETVAERLAANGLDGVLLATATVDSIAPLRLRDRGMPFVYFNRIGSLVGADAAVVDPAA
                     GYQDAVGRIAELGHTRV"
BASE COUNT          300 a          648 c          713 g          320 t
ORIGIN      
        1 ccgcgcagcg aggtcacgac gtcgcctgag ccgtgtcggc cgtcgcgagg gccgcgtcga
       61 tccagtcgaa gtccttgccc tcgtgtcgcc acacgcgggc gtcgcccgag cgggcatggc
      121 cctcgaacag ctcgacccgt gcggcgcggc cgagcacgtc gccgagctgc gcggacgacg
      181 ccgtgtcgac gatctcctga tacgtgacgg tgcgcaggta cttgcccacc cagagaccgc
      241 cggtgtaccg ggcggcgccg agggtcggca gcacatggtt ggtgccgatc accttgtcgc
      301 cgtatgagac gcaggtgttc tcgccgagga acagcgcgcc gtagtcgtgc atcttcgtca
      361 gcgcctcgcg cggttcggcg gtgaagatct gcacgtgctc cgaggcgaac gaatcggcga
      421 tgcggtaggc ctcgtcgagg tcgtcggcca cgatcacctg gccccagtcg cgccacgccg
      481 cttcggcgta gtccttggtc ggcatctcag gaaggatgcg gtcgatcagc tccatgacgc
      541 ggtcggcgag ctcgcgcgag gtggtcacca gcacagcggg ggactccggt ccgtgctcgg
      601 cctgcgagag cagatcgacg gccgtgaaga acgggtcggc ggtctcgtcg gcgacgatca
      661 ggatctcggt gggccccgcg aacaggtcga tgcccacctc gccgaacagc tgacgcttcg
      721 cctccgcgac gtacgggttg cccggacccg cgatcatgtt caccggcgcg atcgtctcgg
      781 taccaacggc catggccgcg accgcctgga tgccgccgag gatgtagatc tcgtcggccc
      841 cggccatctt catggcggcg acggtggccg cggggatctc gccgcggata ggcggcgtgc
      901 aggccacaac gcgggggacg ccggcgacct tggccgtgat gatcgtcatg tgcgcggatg
      961 ccgtcagcgg gtacttgcct ccggggatgt acgcgccggc ggcctggacg ggcacgtgct
     1021 tctgccccag cagcacgccg ggaagcgtct cgacctcgat gtcgatgagg gtgtcgcgct
     1081 gagcctgcgc gaagcggcgg acctgcgcct ggacgaactc gatgtcggcg atgacctggg
     1141 ggtcgagggt gccgatgatg cgctcgatct cctcgtcgtc gaggcggtac gagtcgcggc
     1201 tccagcggtc gaacttctcc gcgtagcggc ggacggcggt gtcgccttcc gcgcggatgt
     1261 cggcgatgac cgcgctgacg gcttcggcga catcgtgctg ggcggtgtcc gcgaacgact
     1321 tcgtaggggc ggacttcaga tgcagggcca tgggccttcc tctcaatgca tacgtatgct
     1381 gagattatga ccccgtatgc atttgcggtc aagggtctag aatccgagga accacaggag
     1441 ggggatctgc atggtggtca cgagccgcga tgtcgcacgc ctggccggcg tgtctcagcc
     1501 cacggtctcg cgcgctctca gagacgacgc gagagtgtcg gaggacacga agaagagggt
     1561 gcgcgaggct gcgcagctgc tgggctacgt gcccagcgaa gcgggcaggg cgctctcgtc
     1621 ggggcgcacg cgacgcatcg gactgctggt cacggatctc gacaaccagt tctactcgca
     1681 catcatcgcg ccggtgcacc gtgagctcga gcggctcgga taccagctca tgctgcacac
     1741 cgagacggcc gacaacgaga ccgtcgcgga acgactggcc gccaacgggc tggacggcgt
     1801 cctgctcgcg accgcgacgg tggactcgat cgcgccgctg aggctccgcg accgcggcat
     1861 gcccttcgtg tacttcaacc gcatcgggtc gctcgtgggt gcggatgccg cggtggtcga
     1921 cccggccgcc ggctaccagg acgcggtcgg tcgcatcgcc gagctggggc acacgcgggt
     1981 c
//
LOCUS       DATMSX010000433         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_167231,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000433 DATMSX010000000
VERSION     DATMSX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167231"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VN041_13775"
     CDS             <1..231
                     /locus_tag="VN041_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWU30146.1"
                     /translation="AAAAAGGVDKLAGVLRVGGFVASDPSFTGQPGVINGASEVLGEI
                     FGDAGKHVRAAVGVTVLPLDTPVEVEVAFLLA"
     gene            complement(297..2267)
                     /gene="acs"
                     /locus_tag="VN041_13780"
     CDS             complement(297..2267)
                     /gene="acs"
                     /locus_tag="VN041_13780"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWU30147.1"
                     /translation="MSSQIDHLLDESREFPPSDEFVADTIDDPALYERAKADREGFWG
                     DQARELLSWSKPFTQVLDWSNPPFAKWFDDGELNVAYNCLDRHVEAGLGDRVAIHWEG
                     EPGDSRSITYAELTEEVKRTANVLSDLGIGHGDRVAIYLPMIPEAVISMLAVARLGAI
                     HSVVFGGFSADSLRSRIDDAGAKVVITADGGWRKGKVSALKPAVDQALSDRHGEGEQA
                     TVEHVLVVKRGENEVDWDADRDVWWHEVVPQASAEHEAQAFPAENPLFILYTSGTTGK
                     PKGILHTTGGYLTQAAYTHKYVFDLHPETDVFWCTADIGWVTGHTYVTYGPLANGATQ
                     LMYEGTPDAPHPGRWWELIQKYGVTIFYTAPTAIRSFMKTGRQIPQQFDLSSLRLLGS
                     VGEPINPEAWMWYREVIGADRTPIVDTWWQTETGAAMVSPLPGITAAKPGSAQVPLPG
                     ISIDVVDDDGNEVGNGNGGLLVITEPWPSMLRGIWGDPERFKETYWEKFQDRGYYFAG
                     DGARLDEDGDLWLLGRVDDVMNVSGHRLSTTEIESSLVAHEATAEAAVVGANDETTGQ
                     AVVAFVIIKQSYLDQHSPEGLVAQLRGWVGEQIGPIARPRDVYIVNELPKTRSGKIMR
                     RLLRDVAEGREVGDTTTLADTMVMTTITSQVK"
     gene            2653..3660
                     /locus_tag="VN041_13785"
     CDS             2653..3660
                     /locus_tag="VN041_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HWU30148.1"
                     /translation="MPEPFVLRPRTTSGLAAVEDVSVPKTVDAAFGALADHVRDPLVT
                     DIFVNGSAGLFIDRGEGAERVTDWRASEREVRDLAVALVGAGGRHLDDQSPCVDVRLD
                     DGIRVHAVLSPVSTSGTALSIRVPRVIGADLDALAASGTFTPGQREWLGRLVADRANI
                     LITGGTGTGKTTLLTTLLTQAGPGERIVTIEDVAELRPRHPHHVALEARQPNLEGAGR
                     ITLAMLVRESLRMRPDRLVVGECRGEEVRELLTALNTGHDGGAGTLHASGLADVPARL
                     EALGALAGMDAVALARQVVSAFTIVLHLERGTDGRRRIARAGRFVLEGERLAMEEVAP
                     W"
BASE COUNT          500 a         1263 c         1316 g          610 t
ORIGIN      
        1 gcggccgcgg ccgccggcgg ggtcgacaag ctcgccggag tgctccgtgt cggcggcttc
       61 gtggcatccg acccgtcgtt cacgggccag cccggtgtga tcaacggcgc gagcgaggtg
      121 ctcggcgaga tcttcggtga cgcgggcaag cacgtccgcg cagcggtcgg cgtgactgtg
      181 ctgccgctgg acacccccgt cgaggtcgag gtcgcgttcc tcctcgcctg acgacgacgt
      241 ccgtacagca gaaggcggtc cctcccgaac gggagggacc gccttctgca tccgtgctac
      301 ttgacctggc tcgtgatggt ggtcatgacc atggtgtcgg ccagcgtggt ggtgtcgccc
      361 acctcgcgtc cctccgcgac gtcgcgcagc aggcggcgca tgatcttgcc cgagcgggtc
      421 ttgggcagct cgttcacgat gtacacgtcg cgcggacggg cgatcgggcc gatctgctcg
      481 ccgacccagc cgcgcagctg cgccaccagg ccttcgggcg agtgctggtc gaggtagctc
      541 tgcttgatga tgacgaaggc cacgaccgcc tggccggtgg tctcgtcgtt cgcgcccacg
      601 acagccgcct cggcggtggc ctcgtgcgcc accagggagg actcgatctc ggtggtcgac
      661 aggcggtggc ccgagacgtt catcacgtcg tccacgcggc ccagcagcca gaggtcgccg
      721 tcctcgtcga ggcgggcgcc gtcgccggcg aagtagtagc ccctgtcctg gaacttctcc
      781 cagtacgtct ccttgaaccg ctccgggtcg ccccagatgc cgcgcagcat cgagggccaa
      841 ggctcggtga tgaccagcag gccgccgttg ccgttgccga cctcgttgcc gtcgtcgtcg
      901 accacgtcga tcgagatgcc gggcagcggc acctgggcgg aaccgggctt ggccgccgtg
      961 atgccgggga gcggggagac catcgccgca ccggtctcgg tctgccacca ggtgtccacg
     1021 atcggcgtcc tgtcggcgcc gatgacctcg cggtaccaca tccacgcctc ggggttgatg
     1081 ggctcgccca ccgagccgag caggcgcagg ctcgacagat cgaactgctg cgggatctga
     1141 cggccggtct tcatgaagga gcggatggcc gtgggggccg tgtagaagat cgtcacgccg
     1201 tacttctgga tcagctccca ccagcggccg ggatgcgggg catccggcgt gccctcgtac
     1261 atcagctggg tggcgccgtt ggcgagcgga ccgtaggtga cgtaggtgtg gccggtgacc
     1321 cagccgatgt cggccgtgca ccagaagacg tcggtctcgg gatgcaggtc gaagacgtac
     1381 ttgtgcgtgt acgcggcctg agtcaggtag ccgcccgtgg tgtgcaggat gcccttgggc
     1441 ttcccggtcg tgccggaggt gtagaggatg aacagcgggt tctcggcggg gaacgcctgc
     1501 gcctcgtgct cggcggatgc ctgcggcacc acctcgtgcc accagacgtc gcgatccgcg
     1561 tcccagtcga cctcgttctc gccgcgcttg acgaccagga cgtgctccac ggtggcctgc
     1621 tcgccctccc cgtgccggtc gctgagcgcc tggtccacgg cgggcttgag cgccgagacc
     1681 ttgcccttgc gccagccgcc gtcggcggtg atgacgacct tcgcgccggc gtcgtcgatg
     1741 cgggagcgca ggctgtcggc gctgaacccg ccgaacacga ccgagtggat cgcgccgagg
     1801 cgggcgacgg cgagcatcga gatgaccgcc tcggggatca tcggcaggta gatggcgacg
     1861 cggtcgccgt ggccgatgcc cagatcggag agcacgttcg ccgtgcgctt gacctcctcg
     1921 gtcagctcgg cgtaggtgat gctgcgggag tcacccggct caccctccca gtggatggcg
     1981 acgcggtcgc cgaggcctgc ctcgacgtgg cggtccaggc agttgtaggc gacgttcagc
     2041 tcaccgtcgt cgaaccactt cgcgaacggc gggttcgacc agtcgagcac ctgcgtgaac
     2101 ggcttgctcc agctgagcag ctcgcgggcc tggtcgcccc agaagccctc ccggtcggcc
     2161 ttcgcgcgct cgtacagagc cgggtcatcg atggtgtcgg cgacgaactc gtcagacggc
     2221 gggaactccc gcgactcgtc gaggaggtgg tcgatctggc tgctcatggc acttcgctcc
     2281 tttgcgcggg ctgttcggcc gcgactgtgc gcgacccggg acacggccga atctagtccg
     2341 gcgtccgcac ggcggcctac tgtcgaaagt cggtagtgcg cctcgtttgc atcggccctg
     2401 ccgcctgcat acactgacct cagccgaatc ttattccggc ttcagcgcgc ccgatacccc
     2461 cgaacgcggg tcgcgcgtgg gcggcatctc attccccccg tgaggtgccg cccttcggca
     2521 ttctcggggc ggtgctctcc ggtggccttc gccgggcgcg ttctccctgc acaaccgcgg
     2581 attccgcagg attgtgcaca tcccgcgggt tcacggcctc ggtgcgtcgg ccggcccgcc
     2641 tagcgtcgtg gcatgccaga accgttcgtg ctccgtcctc gcaccaccag cggcctcgcc
     2701 gccgtcgagg acgtgtccgt gcccaagacg gtcgatgccg cattcggcgc cctcgccgat
     2761 catgttcgtg atccgctcgt caccgacatc ttcgtgaacg gctccgccgg gctgttcatc
     2821 gatcggggtg agggtgcgga gcgcgtgacg gactggcggg catccgagcg ggaggtccgc
     2881 gacctggccg tcgcgctcgt cggagccggc gggaggcacc tcgatgacca gtcgccctgc
     2941 gtagacgtgc gactcgacga cggcatccgc gtgcatgccg tgctgtcccc ggtctcgacc
     3001 tccggcacgg cgctgtcgat ccgtgtgccg cgggtgatcg gagccgatct ggatgcgctg
     3061 gcggcatcgg gcaccttcac gcctggacag agggaatggc tcggacggct ggtcgcggat
     3121 cgcgcgaaca tcctcatcac cggtggcacc ggcacgggca agacgacgct gctcacgact
     3181 ctgctgaccc aggcgggtcc gggggagcgg atcgtgacga tcgaggatgt cgcggagctg
     3241 aggccgcggc atcctcacca cgtcgccctc gaggcgagac agccgaacct ggagggcgcc
     3301 gggcgcatca cgctcgccat gctcgtgcgg gagtccctgc ggatgcgccc ggaccgcctg
     3361 gtcgtgggcg aatgccgcgg cgaggaggtg cgggagctgc tcaccgcgct caacaccgga
     3421 cacgacggcg gcgccgggac cctgcacgcc agcggtctcg ccgacgtccc tgcacgcctg
     3481 gaggcgctcg gcgcgctcgc gggcatggac gccgtcgcgc tcgcgaggca ggtcgtcagc
     3541 gccttcacga tcgtgctgca tctggagcgg ggcacagacg gtcgtcgccg catcgcccgc
     3601 gccggccgct tcgtgctgga gggcgagcgg ctggcgatgg aggaggtggc gccgtggtga
     3661 tgcacctgct gcgccggaga gaaccggat
//
LOCUS       DATMSX010000434         1872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_148091,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000434 DATMSX010000000
VERSION     DATMSX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148091"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VN041_13790"
     CDS             <1..679
                     /locus_tag="VN041_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30149.1"
                     /translation="IALAAVLVIGFSLLPLLPKGTLRAVAGLPSTVLMRGIVAGAFFA
                     AEAYVPKLLIDRFGFSPTVAGLALTLAAIGWSGASAVQGRYGDRLGSARITWIAVLLM
                     LTGVAGLLFVSAIGADPWIVIVAWGLAGAGMGLLYPRLTVLTLAYSTTANEGFNSAAL
                     SIFEASGSAVTIALAGLGFALLPVLGTGYLTVYLLAAALLVLSLVPGLRMGDGAGRTV
                     SVEGAPH"
     gene            complement(654..1202)
                     /locus_tag="VN041_13795"
     CDS             complement(654..1202)
                     /locus_tag="VN041_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU30150.1"
                     /translation="MPPARHDRHSVVDCALRLLDRVGLPDLSMRRLATELDVQPSALY
                     WHFASKQELLAAVAERILDTIPAPDAATSLADTAGSIRDALLAYRDGAEVVMSTYALR
                     LGTRRAQDALGASLGDADQTAADAVFEFVLGHTTLLQQRMHAQSIGAVAPDAADPTAH
                     ADEVFDAGIRAFASLSAARPPR"
     gene            1281..1859
                     /locus_tag="VN041_13800"
     CDS             1281..1859
                     /locus_tag="VN041_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWU30151.1"
                     /translation="MTDSPRPLGADLARIAVFAALIVVLGTVAVPVPGGVPITGQTLG
                     VMLAGLVLGARRAPLAVLVVLALAAAGLPVLAGGRGGLGVFLGPTAGYMLGWIAGSVV
                     IGLIAHHGRFTWWRAGIGAVLGGILVIYLFGIPVQSLVTGVPFGPTVLSSLVFLPGDL
                     LKAVLAVLLAVALRRAYPRAFPTRARTVAHAG"
BASE COUNT          218 a          644 c          682 g          328 t
ORIGIN      
        1 gatcgcactg gccgcggtgc tcgtcatcgg attctcgctg ctgccgctgc tgccgaaagg
       61 caccctgcgg gcggtcgcgg gcctgcccag caccgtcctc atgcgcggga tcgtcgccgg
      121 cgcgttcttc gccgcggagg cgtacgtgcc taagctgctg atcgaccgtt tcgggttctc
      181 acccaccgtg gccggactcg ccctcaccct ggccgcgatc ggctggtcgg gagcgtcggc
      241 cgtgcagggc cggtacggcg acaggctggg cagcgcgcgg atcacctgga tcgcggtgct
      301 gctcatgctg accggggtcg cggggctcct cttcgtgagc gcgatcgggg cggatccgtg
      361 gatcgtgatc gtggcgtggg gtctcgccgg agcgggtatg gggctgctgt atccgaggct
      421 gaccgtcctc actctggcgt actcgaccac ggcgaacgag gggttcaact ccgctgcgct
      481 gtcgatcttc gaggcatccg gctccgctgt caccatcgcg ctggcgggcc tcggcttcgc
      541 gctcctgccc gtgctcggca cggggtatct cacggtctac ctgctggcag cggcgcttct
      601 ggttctctcg ctggtgccgg gtctgcggat gggcgacggc gcgggcagga cggtcagcgt
      661 ggagggcgcg ccgcactgag tgaggcgaag gcgcggatgc cggcgtcgaa gacctcatcg
      721 gcgtgggcgg tcggatccgc agcatcgggt gcgacggccc cgatgctctg cgcgtgcatg
      781 cgctgctgca gcagggtcgt gtgcccgagc acgaactcga agacggcgtc cgcggcggtc
      841 tgatccgcat cgccgagcga ggcgccgaga gcgtcctgcg cgcgtcgcgt gccgagccgg
      901 agcgcgtagg tgctcatcac gacctcggcg ccgtcacggt aagcgagcag tgcatcgcgg
      961 atgctcccgg cggtgtcggc cagtgaggtc gcggcgtcgg gtgcggggat cgtgtcgagg
     1021 atgcgttcgg cgacggccgc gagcagctcc tgcttgctcg cgaagtgcca gtacagcgca
     1081 ctcggctgaa catccagctc ggtcgccagg cgacgcatgg acagatcggg cagccccacg
     1141 cggtccagca ggcgaagggc gcagtcgacc acactgtgac ggtcgtgtcg tgcgggaggc
     1201 atggctccca gtatagtgaa caccgttcag gtgaacagtg ttcaggacgg tgtgacccga
     1261 gccgtccgaa aggagccccg atgaccgatt ccccacgccc gctgggcgca gacctcgccc
     1321 gtatcgccgt cttcgccgcg ctgatcgtgg tgctcggcac cgtggcggtg cccgtacccg
     1381 gcggtgtccc gatcaccgga cagaccctgg gggtcatgct cgccggcctc gtgctgggtg
     1441 ccagacgcgc acccctggcc gtgctggtcg tgctcgccct cgccgccgcg ggcctgcccg
     1501 tgctcgccgg cggacgtggg ggactgggcg tgttcctggg ccccaccgcg ggttacatgc
     1561 tcggctggat cgccgggagc gtcgtgatcg gtctcatcgc ccaccacggc cggttcacct
     1621 ggtggcgcgc cgggatcggc gccgtgctgg gcggcatcct cgtgatctat ctcttcggca
     1681 tccccgtgca gtccctcgtg accggtgtgc cgttcgggcc caccgtcctc tccagtcttg
     1741 tgttcctgcc cggcgacctg ctcaaggccg tcctcgccgt gcttctggcc gtcgcactgc
     1801 gccgggcgta tccgcgcgcg ttcccgaccc gggcgcgcac cgtcgcgcat gccggctgag
     1861 gcggtcgcga cc
//
LOCUS       DATMSX010000435         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_72954,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000435 DATMSX010000000
VERSION     DATMSX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72954"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VN041_13805"
     CDS             <1..911
                     /locus_tag="VN041_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30152.1"
                     /translation="ARVQAAASELAYVAHSSATSLATGRSLSVGVIMPALDRWFFAEL
                     LAGIQEGLLASGQDLVLYGIQEGTAERARLFEEVLPRRRLDGIIAVGIQPSAHELERL
                     IQVESPLVSVGAYSEDTSAVSIDDVAAARIATEHLIDLGHRDIAFLGGRADAATHAFG
                     DEQRRSGYREAMNAAGLAESIRHADGAPTMPGGYEAGVRILGDRQNRPTALVAVCDEA
                     AIGGVIGARRLGLSVPAELSIVGIDDHVHAEMFALTTVRQQPRVQGRAAVDLLRQRME
                     APDAVPERRVLPSELVVRASTAAPRT"
     gene            complement(1011..1394)
                     /gene="rplL"
                     /locus_tag="VN041_13810"
     CDS             complement(1011..1394)
                     /gene="rplL"
                     /locus_tag="VN041_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWU30153.1"
                     /translation="MAKLSTEELLEQFAGLTLVELSEFVKAFEEKFDVTAAAPVAVAG
                     AAGGAGAAEAEEEKDSFDVILEAAGDKKIQVIKTVRELTSLGLGEAKAVVDGAPKAVL
                     EGANKETADKAKEALEAAGATVTLK"
     gene            complement(1442..1930)
                     /gene="rplJ"
                     /locus_tag="VN041_13815"
     CDS             complement(1442..1930)
                     /gene="rplJ"
                     /locus_tag="VN041_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWU30154.1"
                     /translation="MAQKDATVAELTKNFENSNAVLLTEYRGLTVAQLKQLRNDIRQD
                     AEYAVVKNTLTKIAANNAGITTLDDDLKGPSAVAFVHGDFVATAKAMRDFAKANPLLV
                     IKGGVFEGKTLDADEVNKYASLESREVLLAKAAGMMKATMGKAAATIDALREKLETAE
                     AA"
     gene            2295..2972
                     /locus_tag="VN041_13820"
     CDS             2295..2972
                     /locus_tag="VN041_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HWU30155.1"
                     /translation="MTPELEARIIADSRDRVAWLRARARGITATDVAGLSGEASIIRA
                     ADAKLGHGPRFGGNAYTDHGRRREPEIAAWVAATHGILPSSALFRAEVEHRHLATPDG
                     VCVDGEGRVLLAEIKTTNKSWATIPRTYLRQVWWQQYVLGAERTLFVWEEHVDFQPIH
                     DEPKSVWIERDDKEIGRLVALANRLIDELYHRTTGQTPPARGPIAPNRRDALRERDMF
                     RALALSD"
     gene            complement(3013..3471)
                     /locus_tag="VN041_13825"
     CDS             complement(3013..3471)
                     /locus_tag="VN041_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30156.1"
                     /translation="MKPFVRGNRKVLAAVGAIVLVTVATWAAYTFAYTADQMDWDCGG
                     GSCATNDPRSFAMPLALLGTVGAVLLSGYAVGLIGPAITIALPAWFFRMGVLDAVEAG
                     FSPEGSVTAPMVVTTIALIVAALCAVAWIPMLLGDLRVRRRVRERERSAA"
     gene            complement(3519..>4128)
                     /locus_tag="VN041_13830"
     CDS             complement(3519..>4128)
                     /locus_tag="VN041_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30157.1"
                     /translation="TRVRVPLAVNADSMASWFLAPLARVARQHPIDVDLHRDDQDYTA
                     RMLESGEVMAAVTSEAEPVGGSAVTPLGVLEYRAMATADYVARWFPDGVTSDALQHAP
                     FVDFDRRDALQQGWLRAREVDPWSVPRHYVPASHDFSEAVRLGLGWGLIPAPHVSDAL
                     VSLGDPLIRVPLYWQQWNLRSPLLDAIASEVAAEARRVLAPL"
BASE COUNT          649 a         1507 c         1372 g          600 t
ORIGIN      
        1 gcgcgcgcgt gcaggccgcg gcgtccgagc tcgcgtacgt cgcgcattcc tcggcgacca
       61 gcctggcgac cgggcgctcg ctctccgtcg gcgtgatcat gcccgcgctg gaccggtggt
      121 tcttcgccga gctgctcgcg ggcattcagg aaggactgct cgcctccggc caggacctcg
      181 tgctttacgg catccaggag ggcaccgccg agcgcgcgcg gctcttcgag gaggtgctcc
      241 cccggcgtcg gctggacggc atcatcgcgg tgggcatcca gcccagcgcc cacgaactgg
      301 aacgcctgat ccaggtggag tcgcccctgg tcagcgtcgg cgcctacagc gaggacacca
      361 gcgccgtctc gatcgacgac gtcgccgcgg cgcggatcgc caccgagcac ctgatcgacc
      421 tcggccaccg cgacatcgcc ttcctcggcg gcagggcgga tgctgccacc cacgccttcg
      481 gcgacgagca gcgccggtcg ggctaccgag aggccatgaa cgccgcggga ctcgccgagt
      541 ccatccggca tgccgacggc gcgccgacca tgccaggcgg atacgaggcg ggcgtgcgga
      601 tcctcggcga caggcagaac cggccgactg ccctcgtcgc ggtctgcgac gaggccgcga
      661 tcggcggcgt gatcggcgca cgaaggctcg gcctgtcggt cccggccgag ctgagcatcg
      721 tcggcatcga cgaccacgtg cacgccgaga tgttcgccct caccaccgtg cggcagcagc
      781 cgcgggtgca gggacgcgcg gcggtcgacc tgctgcgcca gcgcatggag gcgccggatg
      841 ccgtccccga gcggcgcgtg ctcccctccg agctcgtggt gcgcgcgtcg actgcggcac
      901 cgcggacata gcgctgcgga caaagcagag ggccccggga cgaatcccga ggccctccga
      961 gcgctgtgaa gcgcgggacg atgcgctttc gcgcttcgcc ccagacagaa ttacttgagg
     1021 gtgaccgtgg cgccggcggc ctccagggcc tccttcgcct tgtcagcggt ctccttgttg
     1081 gcgccctcga ggacggcctt cggcgcgccg tcgacgacag ccttggcctc gccgaggccc
     1141 agcgaggtga gctcgcggac cgtcttgatg acctggatct tcttgtcgcc ggcagcctcg
     1201 aggatgacgt cgaacgagtc cttctcctcc tcggcctcgg ccgcaccagc gccaccagca
     1261 gcgccggcga cggcgacggg ggcagcagcg gtgacgtcga acttctcctc gaacgccttc
     1321 acgaactcgc tgagctcgac aagggtcaga ccagcgaact gctcaagcag ctcctcggtg
     1381 gaaagcttcg ccatgatgta tctcctaata aatggggttt gggtgaaaga acgcaggtcg
     1441 cttacgcggc ctcggcggtc tccagctttt cgcgaagagc gtcgatggtg gcggcagcct
     1501 tgcccatcgt cgccttcatc atgcccgctg ccttcgccag cagaacctca cggctctcga
     1561 gcgaggcgta cttgttgacc tcgtcggcgt cgagggtctt gccctcgaag acgccgccct
     1621 tgatcacgag aagcgggttg gccttggcga agtcacgcat ggccttggca gtggcgacga
     1681 agtcaccgtg cacgaacgcg acggccgacg gacccttgag gtcgtcgtcc agcgtcgtga
     1741 tccccgcgtt gttggcggcg atcttggtca gcgtgttctt caccacggcg tattccgcgt
     1801 cctgacggat gtcgttgcgc agctgcttga gctgggcaac cgtcagaccg cggtactcgg
     1861 tcagcaggac ggcgttcgag ttctcgaagt tcttcgtgag ctcggcaacc gttgcatcct
     1921 tctgcgccat ggtcactcct tacgtgtacg gatgccgcgc ggctgcgtgg catcgacctc
     1981 gaccgcccgc atgaaaagca aaaacgcccc ggcgcaagcg cacggagcgt gaaaccctct
     2041 gtgtcacctg cgcgggcccc tgctgagcag tgcttcgatc ctcgtgctcg cgcacgacga
     2101 tgaccggcgg tcttcggttg cccccagctt acggcatccg cccctctccc ccaaatccac
     2161 ccgtggtcct gccgtctccc acctcccacc ttcgagagac agcaccgggg agcggcacgg
     2221 ggagatggcg cgacccgacg cgaaggcggc gtgggagtgc tgggagggtg ggaggcgggg
     2281 cgtagggtcg aagggtgaca ccggaactcg aagcgcgcat catcgcggac tcccgcgatc
     2341 gggtcgcgtg gcttcgggca cgggcgcgcg ggatcaccgc gaccgacgtc gcaggtctct
     2401 ccggagaggc ctccatcatc cgtgcggcgg atgccaagct cgggcacggt ccgcgcttcg
     2461 gcgggaacgc ctacaccgat cacggccggc gccgcgagcc ggagatcgcc gcctgggtcg
     2521 cggcgaccca cggcatcctg ccctcctccg ccctattccg cgccgaggtc gagcatcgcc
     2581 acctcgccac gcccgacggc gtctgcgtcg acggggaggg ccgcgtactg ctcgccgaga
     2641 tcaagaccac caacaagtcc tgggccacca tcccgcgcac ctatctgcgc caggtgtggt
     2701 ggcagcagta cgtgctcggg gccgagcgga cgctgttcgt ctgggaggag cacgtcgact
     2761 tccagccgat ccacgacgag cccaagagcg tgtggatcga acgcgacgac aaggagatcg
     2821 gccgcctcgt ggcgctcgcc aaccgcctga tcgacgagct gtaccaccgc acgaccggcc
     2881 agacccctcc tgcgcgcggt cccatcgcgc ccaaccgccg cgacgccctg cgcgagcgcg
     2941 acatgttccg cgccctcgcc ctgtccgact gaggccctcg gaccgagacc gtcccgccgc
     3001 cgggccggtc tctcacgcgg cggagcgctc gcgctccctc accctgcggc gcacgcgcag
     3061 atccccgagg agcatcggaa tccaggccac agcgcacagc gcggccacga tcagcgcgat
     3121 cgtggtcacg accatcggcg ccgtcaccga gccctccggc gagaagcccg cctcgacggc
     3181 gtcgagcaca cccatccgga agaaccatgc cggcagggcg atcgtgatcg cgggtccgat
     3241 cagaccgacc gcgtatccgc tgagcagcac cgcaccgacc gttcccagca gagccagcgg
     3301 catggcgaac gaccgcggat cattcgtcgc gcagcttccg ccgccgcagt cccagtccat
     3361 ctggtcggcg gtgtaggcga acgtgtacgc cgcccaggtc gcgacggtca ccaggacgat
     3421 ggcccccacc gccgcgagca ccttgcggtt tcccctcacg aacggcttca cacgaaccat
     3481 cctatgaacg gccggaggct tcgacgaacc ggcgagcatc acagcggtgc gagcacgcgg
     3541 cgcgcctcgg ccgccacctc agaggcgatc gcgtcgagca gcggcgaacg caggttccac
     3601 tgctgccagt acagcggcac ccggatgagc ggatcaccga gggacaccag cgcgtccgag
     3661 acgtgcgggg cggggatgag gccccacccc agcccgagcc gcaccgcctc ggagaagtca
     3721 tgggaggccg gcacgtagtg ccgcggcacc gaccaggggt ccacttccct ggccctcagc
     3781 cagccctgct gcagcgcgtc gcgccggtca aagtccacga acggcgcgtg ctgcagcgca
     3841 tcggacgtca cgccgtccgg gaaccagcgc gccacgtagt cggccgtcgc catcgcgcgg
     3901 tactccagca cgcccagagg cgtgacggcg ctgccgccca cgggctccgc ctcactggtg
     3961 acggccgcca tcacctcacc cgactccagc atccgcgcgg tgtagtcctg gtcgtcgcgg
     4021 tgcaggtcga cgtcgatcgg atgctgtcgc gcgacgcgcg cgagcggcgc gaggaaccac
     4081 gacgccatcg agtcggcgtt caccgcgagc ggcacgcgca cgcgcgtc
//
LOCUS       DATMSX010000436         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_120614,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000436 DATMSX010000000
VERSION     DATMSX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120614"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VN041_13835"
     CDS             <1..1386
                     /locus_tag="VN041_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HWU30158.1"
                     /translation="VPAAAIGPFKSALVRELGDLGYYVNELAISDVLLHITIAAERVA
                     AGRALAERTDSASAAVRVVGSAMARLADQHFGVALGDGDREHLANLVLTRVVAPGEQP
                     PADAARIVDAEVVQAVRAEIEQAARDYQVDLVDEAFIVRLALHVQNLLHRAQESAWTR
                     NPLTQSLKSSYPMIFDVAVSIASGLHDRLGVPLQEDEIAYIAMHVGGRLERSRAADTI
                     LTATIVCPGYRELQELLHSSVDRSLGRLIEVVQVDTSMDPDWSGIDTDLVLSTIPAVG
                     TDERVVRITPFLAETDIERVQQAAARIRRARRLSRLREELQRYFSADAFLSPVPDAGE
                     ETIIRMLGGTLTAAGLIGEDYISGTIERERMSSTAFTDALAVPHALQMTARRTAIAIG
                     IADGSVAWGAGRVQVVALAAFSEDDRNAFQTVFEQLVEVFSERESVQRIVRRATGFDA
                     FLDELVAVIDG"
     gene            complement(1373..>1612)
                     /locus_tag="VN041_13840"
     CDS             complement(1373..>1612)
                     /locus_tag="VN041_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWU30159.1"
                     /translation="VSVDDGAHARPVAELARLAQSHLSPVRVTTRSGETADLTSVLAV
                     MDLAIASGDEITFEVADSPEAPALLDALEAVLSRR"
BASE COUNT          237 a          554 c          598 g          223 t
ORIGIN      
        1 gtgccggccg ccgcgatcgg gcccttcaag tcggcgctcg tgcgcgagct cggcgacctg
       61 gggtactacg tgaacgagct ggcgatcagc gacgtgctgc tgcacatcac gatcgccgcc
      121 gaacgtgtcg cggcgggccg cgctctcgcc gagcgcaccg actcggcctc ggcggcggtg
      181 cgggtggtcg gatccgcgat ggcgcggctc gcggaccagc acttcggcgt cgcgctgggt
      241 gacggggatc gcgagcacct cgccaacctg gtcctcaccc gcgtcgtggc accgggcgag
      301 cagccacccg ccgacgcggc gcggatcgtc gacgccgagg tcgtgcaggc cgtccgagcg
      361 gagatcgagc aggccgcgcg cgactaccag gtggacctgg tcgacgaggc gttcatcgtg
      421 cgcctcgccc tgcacgtgca gaacctactg caccgggcgc aggagagcgc gtggacccgc
      481 aacccgctca cgcagtcgct gaagtcgtcc tacccgatga tcttcgacgt cgcggtgtcc
      541 atcgccagcg gactgcacga caggctgggc gtgcccctgc aggaggacga gatcgcgtac
      601 atcgcgatgc acgtcggcgg acggctggag cgcagccggg ccgcggacac catcctcacc
      661 gccacgatcg tctgccccgg ctaccgcgag ctgcaggaac tgctgcactc cagcgtggat
      721 cgctcactgg ggaggctgat cgaggtcgtc caggtggaca ccagcatgga ccccgactgg
      781 tcgggcatcg acaccgacct ggtgctcagc acgatccccg cggtggggac ggatgagcgg
      841 gtggtgcgca tcacgccgtt cctcgccgag acggacatcg agcgcgtgca gcaggccgcc
      901 gcgcgcatcc gccgggcgcg caggctcagc cgcctgcggg aggagctgca gcggtacttc
      961 tccgcagacg cgttcctctc cccggtgccg gatgccggcg aggagacgat catccggatg
     1021 ctgggaggga cgctcacagc cgcgggcctg atcggcgagg actacatctc gggcacgatc
     1081 gagcgggagc ggatgtcgtc gacggcgttc acggacgcgc tggccgtccc gcatgcgctg
     1141 cagatgaccg cgcgtcgcac ggccatcgcg atcggaatcg cggacggctc ggtcgcgtgg
     1201 ggcgcggggc gggtgcaggt cgtcgcgctc gccgcgttca gcgaggacga caggaacgcg
     1261 ttccagaccg tcttcgagca gctcgtcgag gtgttcagcg agcgcgagag cgtgcagcgc
     1321 atcgtccggc gcgcgaccgg attcgacgcg ttcctcgacg agctggtcgc cgtcatcgac
     1381 ggctgagcac ggcctccagc gcgtcgagga gtgccggcgc ctccggggag tcggccacct
     1441 cgaaggtgat ctcatccccg gaggcgatcg ccaggtccat cacggccagc acgctggtca
     1501 ggtcggcggt ctccccggag cgggtggtga cccggaccgg ggacaggtgc gactgcgcca
     1561 ggcgggcgag ctcggcgacg gggcgtgcat gggcgccgtc gtcgacgctg ac
//
LOCUS       DATMSX010000437         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_15902,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000437 DATMSX010000000
VERSION     DATMSX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15902"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1005)
                     /locus_tag="VN041_13845"
     CDS             complement(79..1005)
                     /locus_tag="VN041_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWU30160.1"
                     /translation="MSSVLYDVPGPRAIARNRILAVVTVAVVAAVVGFVVYRMAATGQ
                     FTADKWYVFTFTNVWMSIFKALGQTLGAFALAAVLSLALGFVLAIGRLSEHAWVRVPV
                     TVLTELFRAVPVLVFMMLLYYGLPVLGVRMDPYWAVVLGLMVYNGSVLAEALRAGIES
                     LPKGQREAGYAIGLRKSGVMAFILLPQAIRAMLPVIVAQLVVTLKDTALGFIITYPEL
                     LYYAKLLSSQQGRPILQSAFVIGAIYIAMCLILSGVAKWLEIRTRHSPKLKGYTPGAD
                     DDPRVHDGTVTEVIAMQRGGGKFDAGGVGPTL"
     gene            complement(1002..1658)
                     /locus_tag="VN041_13850"
     CDS             complement(1002..1658)
                     /locus_tag="VN041_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWU30161.1"
                     /translation="MNVIFGNLDLWGEAIGNTLLVFFVGGIIALVLGIIVGAMRVSPV
                     PIARAVGTVYVNLIRNTPLTLVFFFFVFGYPQLGLPDLSNTVLGILAIGIYTATYVAE
                     VLRAGISTVPVGQAEAARAIGLPFGQVMTLVILPQAFRTVVPPMMSVFIALLKNTTVA
                     AGFSIAELAALRATINDAPDRPGNPMEVLLWVAVVFVVLVMVMSTIQRHLENKWRIVR
                     "
     gene            complement(1744..2646)
                     /locus_tag="VN041_13855"
     CDS             complement(1744..2646)
                     /locus_tag="VN041_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWU30162.1"
                     /translation="MRRTKALAGIGIVAAALLALTGCNSGSPSNPGGGGGDNSGGDKP
                     LFEVAKDVKLDGSPTFDAMKKRDGVVIGVKEDQPGLGYLDATTGERTGFDVDIARWIA
                     ASLGFDEKQITFKPIASVNREQSIVNGDIDYYVGTYSITDKRKTQIDFAGPYFVTGQG
                     LLIRKGDPDADKLEDFNGKTVCSATGSTPIQNIKANFPQIKTQEYDLYSACVQDLLDK
                     KVDAVTTDQAILIGFAAQYPKDLKVTGGLFTEERYGVGLKKGDTALQQHINTLFTDGH
                     DTWQKIFDKNLGKSGIKVEQPKVD"
     gene            complement(2697..3425)
                     /locus_tag="VN041_13860"
     CDS             complement(2697..3425)
                     /locus_tag="VN041_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWU30163.1"
                     /translation="MVVVEGVQKHYGEFHALKDINLTVDKGEVVVIIGPSGSGKSTLC
                     RTINRLETITSGSIRIDGKPLPAEGKGLANLRAEVGMVFQSFNLFAHLTILENVTLGP
                     IKVRGVKKADAEKEAMALLERVGVAQQASKLPAQLSGGQQQRVAIARALAMHPKVMLF
                     DEPTSALDPEMINEVLDVMVGLAKEGMTMIVVTHEMGFARKAANRVVFMADGQIVEES
                     TPEEFFTHPKSDRAKDFLSKLLTH"
     gene            complement(3584..>4069)
                     /locus_tag="VN041_13865"
     CDS             complement(3584..>4069)
                     /locus_tag="VN041_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWU30164.1"
                     /translation="VLEASQVPAGRVRDVGLGLDASIAALTDGRIDAFFWVGGLPTPG
                     IARLAGRLPIRLLSIGPDVVERADAAHAGVYRSAEFPVGTYGSDESTVAMTVPNYLVT
                     SADVPDALVSDVLAELFSARAEVARSVPAAALLDRRLAIFTDPIELHPGAGEYYRDSR
                     R"
BASE COUNT          666 a         1320 c         1391 g          692 t
ORIGIN      
        1 gggtgacacg aagagggcgg gcatccgatc ggatgcccgc cctctcgctc ccctccggag
       61 atgtggacgt cggtgagctc agagcgtcgg ccccacgcca cccgcgtcga actttccccc
      121 accgcgctgc atggcgatca cttcggtgac cgttccgtcg tggacgcgag ggtcgtcgtc
      181 tgcacccggc gtgtagccct tcagcttcgg cgagtgccgc gtgcggatct ccaaccactt
      241 cgcgacgccc gacaggatca ggcacatcgc gatgtagatc gcgccgatga cgaacgccga
      301 ctgcaggatc ggccggccct gctgcgagct gagcagcttg gcgtagtaca gcagctcggg
      361 gtaggtgatg atgaagccga gcgcggtgtc cttcagcgtg acgaccagct gcgcgacgat
      421 caccggcagc atggcgcgga tggcctgcgg cagcaggata aaggccatga cgccgctctt
      481 gcgcaggccg atcgcgtagc cggcctccct ctggcccttg ggcagcgact cgatgcctgc
      541 gcggagcgcc tcggcgagca cggacccgtt gtagaccatc agccccagca cgacagccca
      601 gtaggggtcc atccggacgc cgagcacggg aagtccgtag tacagcagca tcatgaagac
      661 cagcaccggg acggcacgga agagctcggt gaggaccgtg accggcacgc gcacccaggc
      721 gtgctcggac agacgcccga tcgccagcac gaagcccagc gccaggctga ggacggcggc
      781 cagggcgaac gcgccgaggg tctgcccgag tgccttgaag atgctcatcc acacgttggt
      841 gaaggtgaag acgtaccact tgtcggcggt gaactgtccg gtggccgcca tgcggtagac
      901 cacgaagccg accactgctg cgaccaccgc gacggtgacg acggcgagga tgcggttgcg
      961 ggcgatcgcc cgggggcccg ggacgtcgta gagcacggaa ctcatcgcac gatcctccac
     1021 ttgttctcga gatgccgctg gatggtgctc atgaccatca ccaggacgac gaagaccacc
     1081 gcgacccaga gcagcacctc catcgggttg cccgggcggt cgggtgcgtc gttgatcgtg
     1141 gcgcgtagcg ccgcgagctc ggcgatggag aagccggccg ccaccgtggt gttcttcagc
     1201 agggcgatga acacgctcat catgggcggg accaccgtgc ggaaggcctg agggaggatg
     1261 accagggtca tcacctggcc gaacggcagc ccgatcgcgc gggccgcttc ggcctggccg
     1321 acgggaacgg tgctgatgcc cgcgcgcagg acctcggcga cgtaggtcgc cgtgtagatg
     1381 ccgatcgcga ggatgcccag cacggtgttc gacaggtcgg gcagacccag ttgcgggtag
     1441 ccgaacacga agaagaagaa gaccagcgtc agcggtgtgt tccggatgag gttcacgtac
     1501 accgtgccga cggcgcgggc gatgggcacc ggcgagacgc gcatcgcgcc gacgatgatg
     1561 cccaggacca gcgcgatgat gccgccgacg aagaacacca gcagagtgtt cccgatggcc
     1621 tcaccccaca ggtcgaggtt gccgaagatg acgttcatgc cacccttcct tcgtgacgat
     1681 gccttcgtgg atgggcgacc ccgtggtgcc ggggccgccc atcactgatg gacagcggcc
     1741 gggtcagtcg accttgggct gctcgacctt gatgccggac ttgccgaggt tcttgtcgaa
     1801 gatcttctgc caggtgtcgt gaccgtcggt gaagagcgtg ttgatgtgct gctgcagcgc
     1861 cgtgtcgccc ttcttcaggc ccacgccgta gcgctcctcg gtgaacagac cgccggtgac
     1921 cttcaggtcc ttcgggtact gggccgcgaa gccgatcagg atggcctggt cggtggtcac
     1981 ggcgtcgacc ttcttgtcga gcaggtcctg cacgcaggcg gagtacaggt cgtactcctg
     2041 cgtcttgatc tgcgggaagt tggccttgat gttctggatc ggcgtggagc cggtcgccga
     2101 gcacacggtc tttccgttga agtcctcgag cttgtcggcg tcggggtctc ccttgcggat
     2161 cagcaggccc tggccggtga cgaagtaggg accggcgaag tcgatctgcg tcttgcgctt
     2221 gtcggtgatg gagtaggtgc cgacgtagta gtcgatgtcg ccgttcacga tcgactgctc
     2281 gcggttgacc gaggcgatcg gcttgaacgt gatctgcttc tcgtcgaagc cgagcgatgc
     2341 ggcgatccaa cgggcgatgt cgacgtcgaa accggtgcgc tcacccgtgg tggcgtcgag
     2401 gtagccgaga ccgggctggt cctccttgac gccgatgacc acgccgtcgc gcttcttcat
     2461 ggcgtcgaac gtggggctgc cgtcgagctt gacgtccttg gccacctcga acagcggctt
     2521 gtccccgcca ctgttgtcgc caccgccgcc accggggttg gacgggcttc cgctgttgca
     2581 gccggtcagc gcgagcagcg ccgcggcgac gatgccgatt cctgcgagtg ccttcgtgcg
     2641 tcgcatggtg ttctccttcg tgtgctgtgg gtgcgcggga tctccgcgcg ggaaggtcag
     2701 tgggtgagga gcttggacag gaagtccttg gcgcggtcgc tcttcgggtg cgtgaagaac
     2761 tcctcgggcg tcgactcctc gacgatctgc ccgtcggcca tgaagaccac acggttggcg
     2821 gccttccggg cgaagcccat ctcgtgggtg acgacgatca tcgtcatccc ctccttggcg
     2881 aggccgacca tgacgtcgag gacctcgttg atcatctccg ggtccagcgc gctggtgggc
     2941 tcgtcgaaga gcatcacctt cggatgcatg gccagcgcgc gggcgatcgc gacacgctgc
     3001 tgctgaccgc cggacagctg cgcggggagc ttcgacgcct gctgcgcgac acccacgcgc
     3061 tccaggagcg ccatggcctc cttctcggca tccgccttct tcaccccgcg caccttgatc
     3121 gggccgagcg tgacgttctc gaggatcgtc agatgcgcga acaggttgaa cgactggaac
     3181 accatgccga cctcggcgcg gaggttcgcc agccccttgc cctcggcggg gagcggcttg
     3241 ccgtcgatgc ggatggaccc gctggtgatg gtctccaggc ggttgatcgt gcggcacagc
     3301 gtcgacttgc cggagccgga gggaccgatg atgacgacca cctcgccctt gtcgacggtg
     3361 aggttgatgt ccttcagcgc gtggaactcg ccgtagtgct tctggacgcc ctcgacgacg
     3421 accagcgatt cggctgtgct cattgtgcct cccatgggac cgaccggtcg aagggagcac
     3481 agctggaatc agagctcccc atcgaatccg ctgacccgtt cagactatgc acggatgcgc
     3541 ggccggacca gtcccttgag tgtttcttga gtgtggtgca gaatcaacgc cgcgagtctc
     3601 ggtagtactc ccccgctccc ggatgcagct cgatcggatc ggtgaagatc gcgagccgcc
     3661 ggtccagcag cgcggcggcc ggcacgctgc gggcgacctc cgcacgcgcc gagaagagtt
     3721 cggcgagcac gtcgctcacc agcgcgtcgg ggacatctgc ggaggtgacc aggtagttcg
     3781 gcaccgtcat agcgaccgtc gactcgtcgc tgccgtaggt gccgaccggg aactctgccg
     3841 agcggtagac gccggcatgc gcggcatccg ctctctccac cacgtcggga ccgatcgaca
     3901 gcagccggat cggcaggcgc ccggcgagcc tcgcgatgcc gggggtgggt agccctccca
     3961 cccagaagaa cgcgtcgatg cgtccgtcgg tgagcgccgc gatcgaggcg tccagcccga
     4021 gcccgacatc ccggaccctc ccggccggca cctgcgacgc ctccagcac
//
LOCUS       DATMSX010000438        34853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_148150,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000438 DATMSX010000000
VERSION     DATMSX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 34853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34853
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148150"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VN041_13870"
     CDS             complement(<1..1114)
                     /locus_tag="VN041_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU30165.1"
                     /translation="MTMLDTLFGLIPAESRGQQWVAEDLQLVNWGGYDGGPHRVRFSP
                     NATLLCGGSGSGKSTLMDAYIALMMPHTTPFNGASNGGVTGRPRGDEQRNILSYGRGK
                     IDESRTEEGTKLRVLRGDGEDTWTAIAMTWRDHDDSRFTAIRAWYIPAGARILEDTVR
                     VRATIDGEFDVARLESAASQRLSDASLRELGLETLGTDREFSARLHAVLGIGAAGSGA
                     KAMSLLARIQAGQQITTVDDLYKRLVLEEPETMATADAVVAHFDELESTRTSMITARQ
                     QVNALEPIAGIRERIADAAEQTRLIDEIGDFADPDSRAALWRAQRRLDLLREVESELG
                     DRKRALDAGIREKKALVDAAEVERDGLLDVLRQSGGD"
     gene            complement(1111..1728)
                     /locus_tag="VN041_13875"
     CDS             complement(1111..1728)
                     /locus_tag="VN041_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HWU30166.1"
                     /translation="MEDDLDELFPGDRGTLDAEIRRVLVHVLQRRFLSAEGNRAEWKL
                     LLEHQQIIESRMHDLYLRLVVDLGRGLAYKQQVRSDEFEVPILLKDAPYNRTETLVLV
                     HLRTVFQRESAAGESAPRIDIEDVEQTVLSYLTDADGSPARQQKAIRAALDRLDREGV
                     IDEETTGRYRISALVEVVLSAEKLAELRAWLRRQQQEQQEREIAG"
     gene            complement(1937..3427)
                     /locus_tag="VN041_13880"
     CDS             complement(1937..3427)
                     /locus_tag="VN041_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HWU30167.1"
                     /translation="MTGTRAEAAYSRSLAAFRTPTLGLLHGRQAPFVIAALSMLFSAD
                     RPSVPIADAHAEVAEALDELRAAGHDDDDRRIPAGTGREVCRTWAKQGWLVQQIDDDV
                     EVYRLSAHAVEALEITGRTGGGRTRVSNSRVRTLLEAVERLSDDAEADPVKRIARLTA
                     ERDALDAEILNIQANGAVPLDDEELYEEAENVLHLARELPADFTRVAESLKQMQRDVV
                     SHLRRDDRPTGDVLREYLQRAKDVMQSTPEGRAFAGALRLIGDADRIDALTEQLHGLL
                     AQPFARFLDPAQRIEFDAIGRRVELGVEEVLAAQRRASHVITGQVKTHDPLRDRQIDE
                     LLRDVIAGLHEWSKDAPSSASVDPVRSLPLIDLGRLRQTVGDVRPPGAPAPLTDADDV
                     EYLEADSRSWGGPYYAELEAFVAGLGAEFDLGEAFAHAPDDTRRPVDLVGLLEIAHRN
                     GMTEHDEISTADTVRPDGTTRRFAFGAVTARSAATVPDATKEDQDA"
     gene            complement(3479..4324)
                     /locus_tag="VN041_13885"
     CDS             complement(3479..4324)
                     /locus_tag="VN041_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWU30168.1"
                     /translation="MTALIVGCGDLGTEVGLRLHAAGERVIGWRRNPPAGPQTGIEFA
                     AVDIVAGCEWPGADLLVIAAAPGRDDGYEDTYLTGLQHVLQSMDAAGAAPRRALLVSS
                     TAVYGSTPGDVDETTPIAAPEGRAAVLLEAERRFLDFFAGSETDAVVLRLGGIYGPGR
                     TRLIDQVREGRAVILQTPAVTNRIHRDDAAAAVLHLLTRAERPEQLYLGVDDAPAELG
                     DVLRFIADRLGMPEPPAGEVERTRGGARRCSNALLRASGFRFAFPDHRAGYAHLLKDP
                     RPRHP"
     gene            complement(4321..4647)
                     /locus_tag="VN041_13890"
     CDS             complement(4321..4647)
                     /locus_tag="VN041_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWU30169.1"
                     /translation="MTTLPSAVQAFIDATNSGDTDAFVAAFTTDAYLDDWGREFHGRD
                     GVRSWDRTDNIGKQSHFELVSVEPGEATGAFVVTLTVTGNGYNGTGPMVFQLRDGLIS
                     SLIISG"
     gene            complement(4691..5434)
                     /locus_tag="VN041_13895"
     CDS             complement(4691..5434)
                     /locus_tag="VN041_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30170.1"
                     /translation="MSTTTQTSRVALVTGGSGGIGKAVALRLAADGFAVAVHYAGNKA
                     KAESVVAEITGNAGQAVAVGGDVADEQDMPTAFAAVEQAFGGVDVVVNTAGVMILGPV
                     ADYSLDDFDRIVRTNLRGTFVVSQLAARTVRPGGAIINFSTSVTRTQFPAYGPYVATK
                     AAVESLSLILARELRGRDITVNAVAPGPTATPLFLEGKSDEAVQGLAKAVPLERLGQP
                     DDIAETVAFLAGPARWVNGQVIFTNGGLA"
     gene            complement(5465..6127)
                     /locus_tag="VN041_13900"
     CDS             complement(5465..6127)
                     /locus_tag="VN041_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30171.1"
                     /translation="MTTRTVILEKAAELLAQSISGEISTRAVCEAAGVTQPVLYRLFG
                     DKDGLLAAVADRVWGEYLEMKRAAEPSSDPLDDLRTGWDNHTAFALRHPHAYRLVFAS
                     SLATRPAAFAEAMGLLQGILNRLAEAGRLRVEPAEAARIVMAANSGVALGLILRPESY
                     PEATASVATRDAVLGALITDAAPGDPLTAAATTLAAHLGSSETFTPAERSLLAEWLQR
                     LA"
     gene            complement(6410..6613)
                     /locus_tag="VN041_13905"
     CDS             complement(6410..6613)
                     /locus_tag="VN041_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30172.1"
                     /translation="MTRKDGRPLVTEHRVTGFDMIDYIAAEDLPPLDTRTACVMRREP
                     VDESWMEPDEGPGDDFDIGEWGA"
     gene            complement(6610..6825)
                     /locus_tag="VN041_13910"
     CDS             complement(6610..6825)
                     /locus_tag="VN041_13910"
                     /inference="COORDINATES: protein motif:HMM:NF023092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HWU30173.1"
                     /translation="MTPSALLAFERRHPGHPPGKEAAIIAELGIPPARFYQLLIRAAR
                     SIDGIRADPITARRVRAMVGRRGRAAA"
     gene            6867..7034
                     /locus_tag="VN041_13915"
     CDS             6867..7034
                     /locus_tag="VN041_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30174.1"
                     /translation="MDDDLDWGAQPRCPEDGILMRDVPGGWECPHCGHVQHAPEVELP
                     PEFDGPSIHGG"
     gene            7092..7445
                     /locus_tag="VN041_13920"
     CDS             7092..7445
                     /locus_tag="VN041_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30175.1"
                     /translation="MGTNKRYADAIDRRANAKYILEVSRKAPLQSLTEKELQMDIYPL
                     TTYPKPERCRVWVRFGPHPFRVEGRVLRSTQLAAGVEFTLEETTFRCWVWGPACEVIT
                     LDDVIADRRAHGGLA"
     gene            7476..8171
                     /locus_tag="VN041_13925"
     CDS             7476..8171
                     /locus_tag="VN041_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWU30176.1"
                     /translation="MCGRFAMDAKTDELIQQFVADGGKPEDWWKDFHGSYSIAPTDDA
                     VIVRDRGEGRILELVRWDWQKPANRPKGAPIINARIEKLDTGFWLPAFTAARCVVPMR
                     GYFEWTGEKGDKTPHYLHGDGLLAAAGLTWSMELPNGERSRCMVVVTREARDAGGEVH
                     DRMPAFLTADAMDAWLEPVKLDRGDRDGMLSMLDAASAEVASTITQYVVDRKVNNSRT
                     VDTTDPTVIAPAA"
     gene            8182..8418
                     /locus_tag="VN041_13930"
     CDS             8182..8418
                     /locus_tag="VN041_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30177.1"
                     /translation="MLTGRIRPVGTRTLEVDGTHDEIVAALHAAAVDGWEINSITTRR
                     LVRYGETTTVEAADIDGVRAKVPDGHQLLSVTAS"
     gene            complement(8475..8855)
                     /locus_tag="VN041_13935"
     CDS             complement(8475..8855)
                     /locus_tag="VN041_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30178.1"
                     /translation="MNRHEATRRVMHSIMRPDSAVKLRYVAADGSRPVVVCTPGEYLS
                     DVTKRIDAVGGSANVIMAEVRRFKVMVIEAVPDGVEADSEGVNAELSMGMFYLRANSK
                     ARRTGTFHIGGVNVDSATQAFEYA"
     gene            complement(8852..9220)
                     /locus_tag="VN041_13940"
     CDS             complement(8852..9220)
                     /locus_tag="VN041_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30179.1"
                     /translation="MSEREQKVAAWAPPPLDDCDEDRWSVGLDETHVLSVRRVTWNEQ
                     LVAFAVVLSRKIGERSLEIVCIDTLNHGCVHRHDGDHNAPFKAIRAISSQQDVQESFH
                     DAYDEVYNAYLRTMGLDELR"
     gene            complement(9331..9681)
                     /locus_tag="VN041_13945"
     CDS             complement(9331..9681)
                     /locus_tag="VN041_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30180.1"
                     /translation="MDITLPTIPAGVLVLLGLVSPFAVALINGAVKAIRKPWQKKAVA
                     ILVAIILTAVVLWFYYAYTGDVVPQWPALVLLTIVVGQFSYSMVTKTPASALEQRISP
                     TITVHESPDTFPRR"
     gene            complement(9690..10058)
                     /locus_tag="VN041_13950"
     CDS             complement(9690..10058)
                     /locus_tag="VN041_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30181.1"
                     /translation="MGMIESADIATIAGAVCILIGVLIGHRVADRKSRREAHVAAAAA
                     EVASAETSATAQDRFIDQLQEELKRYRDENDQRVDKLEDRVDKLTEENQAYRSFIGIQ
                     RDHMAAHNIPLPPWPEGLPR"
     gene            complement(10049..10201)
                     /locus_tag="VN041_13955"
     CDS             complement(10049..10201)
                     /locus_tag="VN041_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30182.1"
                     /translation="MAVVKRGTNVQHGQARRHPIPLREWIGLGVLLAIGVTALMLMCT
                     PCWGWA"
     gene            complement(10189..11706)
                     /locus_tag="VN041_13960"
     CDS             complement(10189..11706)
                     /locus_tag="VN041_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWU30183.1"
                     /translation="MPQKLPMPFGNPSTYAGHSGVDFAQPRGKAIKALGRGKVVSIHY
                     TELGGWWTTVAYDVGCTLGNGHQDRKPTIVSVGQIVNEGQDIGFVGSLGTRSTGPHLH
                     ETNKYNQTYAGTMAWLDPNRVVGQSAPTGGGTVSSNWPARELYGEAWVREAQRKLALL
                     GLYSGDNDGKDGPGTQAATKVLQLAGGLKDDGVYGPKTNALADLILAGHNNIESRPVS
                     DIQRVIGVVADNIWGGKTSFGCYVWQRKNGLTADAIWGPASDAKAFPKTTEPAPSKPV
                     LPARVAREITYPEANRAWTPPYAYRIKDGQPNVRSQKITGIAIHHTGNVVDQESFFTS
                     QNDRISCPNLYINARAEEIELVPYHLKPTSTGAADEYCIAIEVQDVTDAPTWGISDAQ
                     HEVIAQFVAWVSRQTSLGGVQVAVPLDRQHVKGHREFAESTNGTICPGPSMRLDWIVE
                     RAKQIVNPPAPEPEPDMVTVPRTELEKLRDDLAGMGSEVATAFGELAAKPAGWLS"
     gene            complement(11771..12025)
                     /locus_tag="VN041_13965"
     CDS             complement(11771..12025)
                     /locus_tag="VN041_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30184.1"
                     /translation="MKEFLKPILTQIAKLWAALDKKATYRWGTVTQTAPLLVHLDGDI
                     DAAGDPVDVAPQSVLSSIPVGARVACIEQDRRVIVIAVAS"
     gene            complement(12022..13044)
                     /locus_tag="VN041_13970"
     CDS             complement(12022..13044)
                     /locus_tag="VN041_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30185.1"
                     /translation="MLRWSFDLLDAQDRPLREVSPAVGGGSADIVAQSPLGGSGKLLL
                     DDLGQGIDFMEHRLRATFHSDAASWPVGTFLMTSPVEKHTRFKLGWEVGLLTKMNIPA
                     EDTTDARFSLAVGEPVIPAVVALLQSTGETRIAVTPSDKVRTAEVTYPAGTSKLKIIN
                     ELLQSIGYWSLWCDGSGQFRIEPYADPASRPVAFEFQHGEDSLHFPEWDREQNLSDVP
                     NKVTLVTRGDEDSEGLIGTAKNEDPASPFSFQARGHRWIGPEAETVEAESQAVLDQLA
                     ARKLADLMAPVARLKVTHAMLNLDPNALVAFTPEDGRRRLATIQRMSMNFDPFTDISA
                     EWREVA"
     gene            complement(13044..13766)
                     /locus_tag="VN041_13975"
     CDS             complement(13044..13766)
                     /locus_tag="VN041_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30186.1"
                     /translation="MATNIGFDDGHPYFETGADDGKVFIVDGRIAFTDTDATPSTVTV
                     ERSDDGGSTWTPVLGETPAEVGAAFMDWQSASYADILYRAIAVNTEGAAAVTTITVEA
                     RSLALWLSGGVGFGQTARLPLNPDVTHTTSRDGALKEYAGRDKPVAYRGEMTTRSVAV
                     SGRVTDRKFIQDTVTPDQLLQMALSPEPIFLFRDPDGRRIYGTIGPVNMPRQTTARDD
                     DGMRSWNGFWGYSFTLTEAEEA"
     gene            13765..15021
                     /locus_tag="VN041_13980"
     CDS             13765..15021
                     /locus_tag="VN041_13980"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWU30187.1"
                     /translation="MAVTLPRADGDDSARLRAAGRGSAFGSDCAIWHPESVGGRIESL
                     DGLRGVAALSVLIGHAMWVTPAFADAVQLSNAGEADFITALFTYTPLHLAWLAREAVI
                     VFFVLSGFVLARPFLSRGRSYGAYYPQRVIRLAVPAWAALAVAALIAVMLYPTTGTPH
                     SELLASQVAPHVEWGDFAGAWLIGGAPQVLLPLWSLKWEIAFSLLLPLYVLIARWAVG
                     VWKGGAVAVVCLGLSAAGTYFSVDELAYLPTFMLGCLVASNQQAITRRIRDSRATWLG
                     VGSLIALAAGWYPRGVSDSTMLTGAGQVLSIVGAAGVVIAALTWRGFAHALSGSRAML
                     WLGDRSFSLYLVHFPVIMVAAFLTLGENLAITLGIGLPVSLLVAEVFFRTVEKPSTDL
                     ARMVKRRLASKSSAAEAEAPAAQRQS"
     gene            complement(15012..16811)
                     /locus_tag="VN041_13985"
     CDS             complement(15012..16811)
                     /locus_tag="VN041_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30188.1"
                     /translation="MVRKYAITEDEFLGMHIDPADGHLKTVRDNGDPVDFGTAMGPSV
                     TDSGVAEFIADPGSLTATQLSATMADEAADTGSALAAQIGLNIERATQTIDARRFGVA
                     AGTTADNTVALQAALDAAADSRLALSLPPGKIKVQSQVRWKHRLHSIIGTQSTSLDFT
                     GATSGPAILMDPTGADATLPRTFMADLSISGPTGIGTTVDGISHLTDFDTKWAEFRRV
                     RISGFRDQVSLKTNSYLWSFEGVTFDTAVRNSVRLAGTNMGENFNFLNCTFQNNTNAP
                     FIFLEPENGSSMFLRGCSLDFGAQIAEIQGGSIEVASCHMEGRYSGAWFTIKAGGSRP
                     SALLIRGGAVIVQPKVVNTGVCHLLVRIDPTTVKPDESVVVSVDGTSVVDRSSLGMRM
                     LRDTSTQPDRNMITMRPVIGMVENGMRISMGVESSLVRNSSLSTALTLTTGAPTISDD
                     SFVSGMSSAYTFDAAKVGRDGTVGAAKFDLAATGRTTILSKRFRVPDDRRPVLFSLAT
                     ACEHLTTTGSALEYLVEWYSAANIATSLGGERGYPEALRFSYNNLTDVTRDWLTGDLL
                     VMPPRGAGFCQIRFCATNSTGTLWVRDMEATQL"
     gene            complement(16815..18398)
                     /locus_tag="VN041_13990"
     CDS             complement(16815..18398)
                     /locus_tag="VN041_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30189.1"
                     /translation="MVTIQVSKSSPYVTFTVPADRSSTNIGGNSSTMWAYLQMSKGSG
                     SFFNGDGLQIASIDGVGEFARYGASPFLPSGMTGWNYGAYYLTASHNADGTRSVTVRM
                     TLDYGTINESHTAVLVLPTIPKTPTGLTVTRVSDTQQTLNWASASVNTSAVVQRSTDG
                     GAWQQVGAPSGNAKTFTDTTTTGNHRYEYRGAVVAGAGQSSWSSTATIYTTPSVPAAP
                     SAARDGMDIVISGSFSAIATGVDVQDGATVVASNVTLPWRDVAPDPGTPHTYKVRAVR
                     GSLASPWSPASNTVQLIAPPNPPVSLAPNGGVVASDVPVVLSWTHNPVDSSTQSAFEL
                     QWRPSGGAWTTVSGTTASDTSVTLPTGGFEWQVRTKGTDPSFSDWSAVATVTVIDRPG
                     VAVTQPADVWPASTLEVEWSWFQAQGRPQSAWRVELLDATGQVVESRDGSGPTSSYVL
                     AKRLTEGQWTVRVMGATGGVWSIFGTETFMVAFVPPEPPELSGVWDDMQGGVALTLAG
                     DESAVAVLDGDAWYVEAGA"
     gene            complement(18461..18721)
                     /locus_tag="VN041_13995"
     CDS             complement(18461..18721)
                     /locus_tag="VN041_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30190.1"
                     /translation="MSKFQMIPSAVAAAAAWDEGVQSSGSFEITDLDLMVLSAASRRE
                     IQSELVERSHAAGYVLEWRRDHPRATVVFTWRRIGDVRKTSV"
     gene            complement(18732..21572)
                     /locus_tag="VN041_14000"
     CDS             complement(18732..21572)
                     /locus_tag="VN041_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HWU30191.1"
                     /translation="MAKLTVADLEVLFTANTTQVEKAEKQIVAVGKKVESKPIQVDAN
                     AKPALDSMDRVEDAARKLVSKDTALKLDADISRAEKSLERTKQRLEDLQVRALGGLDV
                     GADVKRAEAQLARTQRTLDGLVKAKNEIQVEVDEQKAESGLKRLVSLFKRKSEEAGDE
                     GGRSLSKNLDAATRGAGEKVGEVVGGDIENTLVDALSAIPIAGGIILAGVAIGKAITG
                     AINDGLQVDARQDRLQALTGISAADALRLGRAAGEAYANNFGESIETNMDTARLALQF
                     RILDPKATTRDAQLVVQGLAGIADVLGEDVRPVAQAVTQLLSTGLAKNAQGAYDIIAA
                     GARNGLNRNEDLIDTLTEYPSLFKRLGLSGEEALGLINQGMQAGARNSDLAADALKEF
                     QIRATDASVSSAAGFKALGLNAQDMTAQIARGGEDARNGLALVLDKLRETEDPVKRNA
                     AAVALFGTQAEDLGDALFAMDLSNAVDQLDGVAGSAQRMFDQLGDNTAAKVEKAQRSI
                     ETAADGIKGALAVAFADPLGDFADWVSKNRGPVLQFFSDLINGAIDFAITANTGFGEF
                     VSGPLAAMVKGLSSVIGFFDHDAGTKIEAFANEMETFSDSTDVVNDSLEKMRGKFNGF
                     ADGQVALGYLSDASMRTVDAITRVGVAADGTKLSLGGVDTSFLAASESGAKLKAQLEA
                     AGQAFEDEIGLAREAGESQQNLTDRYNTTRQALLDQMVAMGLTQEQAQSLIDTVLKTP
                     EQMETEFKSNADAEKGKVIDLGTKIITLPDGSSTISVDTAGAQGKIDRFISLNNGKVV
                     KVYVDAEGRQSYKIGGKTISAQADGGIVMPMASGGSLTPMAPRAQAVPPNTWRVVGDR
                     MRDREFYIPDDGSAQSMSVLLEAMRSFGVLPMADGGVVGAAGPGGQQLVVYVQSPFTG
                     DYLIAQVADIADARVAGAFPSAESVRSDLPFG"
     gene            complement(21576..21896)
                     /locus_tag="VN041_14005"
     CDS             complement(21576..21896)
                     /locus_tag="VN041_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30192.1"
                     /translation="MTAEDWAPYGIGEPDADGWYPDYRPVPIKLKPAHDGPAAPSDES
                     QIDTSWLAIVQHWRIVVAELAREFHIDIYDPDVRRRPWPGVRTMIFALLDSPSRLRQA
                     LTRR"
     gene            complement(21893..22300)
                     /locus_tag="VN041_14010"
     CDS             complement(21893..22300)
                     /locus_tag="VN041_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30193.1"
                     /translation="MGTAPDFAAWAVPDLVLPLGERTYTVRPPSVDAMGKLLACAVRG
                     EVNLGIVEGEIPDEVQGILDTIGPKEHPALGATYQQLVDDGIDPWTIDKMAYYAVFYW
                     TRGKEYADELAVLLFAPRDMDEPGEAEAAPKGS"
     gene            complement(22305..23063)
                     /locus_tag="VN041_14015"
     CDS             complement(22305..23063)
                     /locus_tag="VN041_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWU30194.1"
                     /translation="MSNKVTLPAGTVLGKSYEYGLDVNLGTYADPTWQSVRRMSAWQP
                     STPPITTDAATYDDLGSPNEDVTGRGFNASLTVQGNRSLTTGLYLPELEAMVAAAKAK
                     GEGAVLDVRYYHKPEIGTPHPTDAGRGFVTVDITRSNTDNTGIETKAITLTGKGEFEQ
                     IPNPFQGWDVTAPAISGISPSGAASGDLVTINGSGLLGASSITFDAIPSDDFQVINGS
                     TIVALVPTDTAGAVPVIITTPGGASPAFTYTRGA"
     gene            complement(23060..23473)
                     /locus_tag="VN041_14020"
     CDS             complement(23060..23473)
                     /locus_tag="VN041_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HWU30195.1"
                     /translation="MDDETLTTRLCEIVGAVPSFAWRPYGPNYTDTEIGVFYGTIPDA
                     PDRAVGIRVYSPIDEPNLLSRRVQLYVRGRRHQPFGADRIADVLFAVLHERPRGDGMA
                     SIFRTSAASLGADDNGREERTENYLITLDNLEASS"
     gene            complement(23466..23783)
                     /locus_tag="VN041_14025"
     CDS             complement(23466..23783)
                     /locus_tag="VN041_14025"
                     /inference="COORDINATES: protein motif:HMM:NF016754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HWU30196.1"
                     /translation="MVELKPVLSMVEKAAQDAMRKGGRTLLKRSNELAPRDDGDLITS
                     GKVVVDDLSVTVRYTAPHAVFQHEHLDWQHDDGGAKFLERASDDVDLASIVADAVKAA
                     LDG"
     gene            complement(23794..24123)
                     /locus_tag="VN041_14030"
     CDS             complement(23794..24123)
                     /locus_tag="VN041_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30197.1"
                     /translation="MTWPESFFFPDTVKVRDYQAGGGMGGGYGNTRTLAAEVIDVQEI
                     VRNAAGEEVISSTRVTVHLEANVMLGSLVTVWSAVMGAEREAEVLRIGRDVNPPPLPS
                     HQILWLK"
     gene            complement(24120..24482)
                     /locus_tag="VN041_14035"
     CDS             complement(24120..24482)
                     /locus_tag="VN041_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30198.1"
                     /translation="MAITPDELGGDENTARRILVLARSIAPCIDSLEDEAKKDAIAIL
                     KGLLDEVPAAGSRRTRSLSRNGTSMTFADITTVFDDDARQALQSLCGVTSTAGGGVPA
                     GSFPTERPASVLWPETYR"
     gene            complement(24503..24802)
                     /locus_tag="VN041_14040"
     CDS             complement(24503..24802)
                     /locus_tag="VN041_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30199.1"
                     /translation="MAAEQKAAADEAADEKQAADEKAKQDAATKAAADKAAADAKATA
                     STKKSKDFKVVGAAVVLRSEDGSERYLYRGAPVDPAAWSKDSIKHAIAVGLIAEA"
     gene            complement(24802..25722)
                     /locus_tag="VN041_14045"
     CDS             complement(24802..25722)
                     /locus_tag="VN041_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30200.1"
                     /translation="MASYTYPVKHPEGTLTTEQLHTLLANPRVIAKRLHDLTQMKFIA
                     DFLLQGRFDASGGGVFYETGEDAFASDDPESIAPLGEYPKVVLESGEVVSARTVKWGL
                     DTVISDEKIKRQGISFVNKGLTRLGNTIVRHVDSVAMAVIAAKVTSTFASPSTWTTAG
                     KAVEAIMSIQATRAALGLGIDLSTIVLTPAQYAKVIGLLIDDKALPRESGATAVQGNI
                     PVDVLGLTWAASPNYTGANPLLVDREQLGGMADEPLGGPGYVGAGQVGIEAKSIRNDD
                     DDSYTLRGRRVTVPVVTEPLAGVQLTGTGL"
     gene            complement(25739..26104)
                     /locus_tag="VN041_14050"
     CDS             complement(25739..26104)
                     /locus_tag="VN041_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HWU30201.1"
                     /translation="MADYIPKFKPGQAVTFKASAAVTGGRLVAITGARTVGPAGADSA
                     AVVGVAGFDAAIGEDVTVFTRAGGVHSLTASGAIVAGVKVSAAATGKVQTVGSTTNPI
                     GLALTAAAADNDVIDVLFI"
     gene            complement(26106..27386)
                     /locus_tag="VN041_14055"
     CDS             complement(26106..27386)
                     /locus_tag="VN041_14055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF045542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HWU30202.1"
                     /translation="MTRAETWPNRFWGSHDLPKTKREFFDAVVAPPPVGDEPEVATIR
                     LYGPIDSWGGFWGISAKDVGGVLDALPESVSQIILRINSPGGHVFEGISIMNLLRAHR
                     AKVTAVVDGLAASAASVIAAGADETVMSPGTQMMIHCTSTIVWGNAADMRKEAAVLEG
                     LDRSLAEIYASKAGEKDWASLLEAETWLTAADAVSEGLADRIAVIPDAGEAATVGDDD
                     VIVVVPDEEEPEDSASARVIRFAQHPTMAAAAAPKLPSSSEPGDPNRKENVVAYDDFK
                     AGIRERLGMTDADATDEAVIAALDEALEEQAEAPVAAATTIPAGTTLIADDVLAELRE
                     NAQAGVEARAQQVNERRDGIIATALSEGRITPASAPSFRAMLDADETAATATINSLAK
                     NTVPVAEIGHTAGEVSAEDALMAQAGWGDENEEA"
     gene            complement(27383..28666)
                     /locus_tag="VN041_14060"
     CDS             complement(27383..28666)
                     /locus_tag="VN041_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30203.1"
                     /translation="MPDEIGYVTDALPGWGDLTAEAHEKNPLLQWPYSIEIFDQMRRE
                     DAQCGSVLRAVTLPIRSTRWEIDPAGARDEVVQLVAEDLGLPIKGQQSAPRPKRGRGR
                     FSWDEFLRLALLELPFGHSVFEQVYRIENGRARLGKLAWRPPRTISKFDVAKDGGLIA
                     IEQYGFSGSGKVRIPVDRLVVFVNEREGANWLGTSLLRQAYKDWALKDKFLRIELLVA
                     ERNGLGIPVYKGAPLPDAVSKDATKAEKWLESQRDEGLKLTTEFRAGEAAGAYIPHGA
                     ELSLIGVTGKLPDMAGPIKYHDEQIARAVLAHFLTLGGDDSTGSYALGDTFADFFTGS
                     LNAVMEHQADVVQQHVVEDLVDINWGPDELAPIISPSRLGKDHPLVAESIRALVDAKV
                     ITPDETLEKHIRASAGLPAPDPATARTGVDLKEQA"
     gene            complement(28681..30360)
                     /locus_tag="VN041_14065"
     CDS             complement(28681..30360)
                     /locus_tag="VN041_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30204.1"
                     /translation="MARAKSHLGPDYRAAVGAEMPTDGVPQYGFTEPRIVTTPLCELT
                     PETSRGYEVIDFAHDVLNVVLFPWERALLIRMLELDAFGLLRFRKALVIVGRQNGKTL
                     MAAVLAAYWLYVDAGRWPNQLPEQNFIVVGAAQKLDIAMKPWKQVRRWAAPDDLKVGI
                     AIDRVPDLQEITYPPRTTNGETELRTHAGAAYLPRTFDGARGQSSARLILDELREQYD
                     YEGWSAIEKSANAMYDSLLVAFSNAGTKRSKVLKGVRETAHAGVDDPETQWFVAEWSA
                     EQDAPLDDPRAFAQANPSAGYLPGMTIAGLMRAAAEAPEKNVERIEVLGQWVTATVDN
                     YIDTATWKSRHVTPADVLAMIPKGARTVWAIDTSHDRQRNWLAAAVYTEDGRPFATIR
                     VKRPGWAWVVPFLIELAQQSGHREVALQSKGVPAMDFIKPLQDAEFEVDGRTEKFIVH
                     QVEWSAFALATGRISDRVRDGDLLLIEQPDVDRAIEGAVIRSYAENIGWSREKSMPVD
                     IAGVCAMTIALYALEVLEPEPEPEPVKPPPAVTVPRSTVASTEPNARTMQF"
     gene            complement(30377..30760)
                     /locus_tag="VN041_14070"
     CDS             complement(30377..30760)
                     /locus_tag="VN041_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30205.1"
                     /translation="MVTQFERQSVSEALERSIANSKHLRARHSAAISAARALARKIDA
                     WDQIVDWALQDAALGKGRPAVPANDNVSIASFLKYLDALGLTPEDEAPAAGPGRPASK
                     PAPAGPAASNPGNKVLAFRKKAASG"
     gene            complement(30938..31282)
                     /locus_tag="VN041_14075"
     CDS             complement(30938..31282)
                     /locus_tag="VN041_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30206.1"
                     /translation="MNKYTAAGLIAEARRGRDVAVITSDVHSAMEDLQRSPGMIGATV
                     RRANGLEEIRAHGDSGRIIVRRPGQGMRGLTADVVFLDADLVPRGFDSMDLYQDALAI
                     IHASPDGEIVRA"
     gene            complement(31292..31612)
                     /locus_tag="VN041_14080"
     CDS             complement(31292..31612)
                     /locus_tag="VN041_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWU30207.1"
                     /translation="MSKRTHTREDQRLRTEFFEEGRRLDADPETRHLADCHLCRTRID
                     YEADPGTTPDSHTLDHYYPVDDYPELQNDPDNFRHAHFSCNSSRGKGDVNENGLGEQM
                     PAWW"
     gene            complement(31975..32178)
                     /locus_tag="VN041_14085"
     CDS             complement(31975..32178)
                     /locus_tag="VN041_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30208.1"
                     /translation="MSAVVVEVDRDECDACPHDAHVQAFVYVSREDWPASLAYCAHHG
                     GRFMDRLVELDAHVIDMRHLLSS"
     gene            complement(32598..33149)
                     /locus_tag="VN041_14090"
     CDS             complement(32598..33149)
                     /locus_tag="VN041_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30209.1"
                     /translation="MAEHDHITKTDTLGCPACDKLAGRDRVPAPMPEQPKMPDLSAFP
                     SVQHRIEGGKFICPGDESAWCHQYPEDDCDHEYWPCGHEFTSHAECWIRPWIDATDLA
                     DSYAGVEHELLLRDEDFPDGDVDWTWEGEYVTFDYARPVGTLGPDCRDGKHDACDGRA
                     LDEATDEIVDCACPHHSAESGAS"
     gene            complement(33142..33297)
                     /locus_tag="VN041_14095"
     CDS             complement(33142..33297)
                     /locus_tag="VN041_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30210.1"
                     /translation="MPDEIRREAEAVALRVYPERHHIHMRRCYADAYERGFRAATDRP
                     DGSKSDG"
     gene            complement(33290..34009)
                     /locus_tag="VN041_14100"
     CDS             complement(33290..34009)
                     /locus_tag="VN041_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30211.1"
                     /translation="MADDYTPSEARICIDFCTAAVRRGRPDGSFMSMEEADAAFFRWL
                     AAHDAQKRAEWEAEQEADDEPCKAEVWSWDIFRHQEVDPYWNALSPRRAAREARELGD
                     RRDMAGGTERREPMNIAQQVTDDMVAEALDAKASMSKLHCYLYSTEKCRCGEEWTDAH
                     WMRFILERALSRAEWEAEQGATVQDLRAARAETWDEAIATVFAWWSAPEGERPATIVN
                     PYGVGLPVDETPNRPDGSRDA"
     gene            complement(34002..34196)
                     /locus_tag="VN041_14105"
     CDS             complement(34002..34196)
                     /locus_tag="VN041_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30212.1"
                     /translation="MSKGHMVWKDKRIPVREVVERPRTDEHGVRDGWNATAGEGPIES
                     RMFYPADMFTFEPEEGADRG"
     gene            complement(34193..>34853)
                     /locus_tag="VN041_14110"
     CDS             complement(34193..>34853)
                     /locus_tag="VN041_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30213.1"
                     /translation="NSHEDTAEWVRQYLFRRPVSPEPITEEQINAERARQIEKGHTPE
                     HDRKHGIAHVLRYVLHYGSRGEHLKAYAMVEAALEIAPSVGVQGEPKLIECPHWSPGR
                     VTMRKSCAACEAVQDEPQVMLRRANGSPKSNDEIRAEAAAIRDHVRALTGLTDAGLTE
                     LGGFQGEPTDAQADAAQKAFHEFTFGPDDDFLTPRRMGITRQAWKAALRAAFNETGEK
                     R"
BASE COUNT         5257 a        11767 c        11764 g         6065 t
ORIGIN      
        1 gatcgccgcc ggactgccgc agcacgtcga gcagtccgtc tcgctccacc tctgccgcgt
       61 cgacgagcgc cttcttctcg cggatgccgg cgtccagcgc gcgcttccga tctccgagct
      121 cgctctccac ctcgcgcagc aggtcgagcc ggcgctgcgc gcgccacagc gcggcgcggg
      181 agtccgggtc ggcgaagtcg ccgatctcat cgatcaggcg ggtctgctca gcggcatccg
      241 cgatccgctc ccggatgccg gcgatcggct ccagcgcgtt cacctgctgc cgggcggtga
      301 tcatgctggt gcgggtgctc tccagctcgt cgaagtgcgc gaccacggcg tccgccgtcg
      361 ccatcgtctc gggctcctcg agtacgagac gcttgtacag gtcgtcgacg gtggtgatct
      421 gctggccggc ctggatgcgg gcgagcagac tcatcgcctt cgcgccggat cccgccgcgc
      481 cgatgccgag cacggcgtgc agtcgggcgg agaactcgcg gtcggtgccg agcgtctcca
      541 gaccgagctc tcgcaggctc gcgtcgctga ggcgctggga tgccgcggat tcgagcctgg
      601 cgacgtcgaa ctcgccgtcg atcgtggcgc gtacgcgcac cgtgtcctcg aggatgcggg
      661 cccctgcggg gatgtaccag gcgcggatcg cggtgaagcg cgagtcgtcg tggtcgcgcc
      721 aggtcatcgc gatggcggtc caggtgtcct cgccgtcgcc gcgcagcacg cgcagcttgg
      781 tgccctcctc ggtgcgggac tcgtcgatct tgccgcggcc gtaggagagg atgttgcgct
      841 gctcgtcacc gcgcggacgg ccggtgacgc cgccgttgga cgcaccgttg aagggcgtgg
      901 tgtgcggcat catcagggcg atgtaggcgt ccatcagcgt ggacttgccg gagccggagc
      961 ccccgcacag cagggtggcg ttgggtgaga accgcacccg gtgcgggccg ccgtcgtagc
     1021 cgccccagtt cacgagctgc aggtcctcgg caacccactg ctggcctcgg gactcggcgg
     1081 ggatcaggcc gaagagcgtg tcgagcatgg tcatccggcg atctccctct cctgctgctc
     1141 ctgctgctgg cggcgcagcc aggcgcgcag ctcggcgagc ttctcggcgc tgagcaccac
     1201 ctcgaccagc gcgctgatgc ggtagcggcc ggtggtctcc tcgtcgatca cgccttcgcg
     1261 gtcgagcctg tccagcgccg cacggatggc cttctgctga cgtgcggggc tcccgtcggc
     1321 atccgtcagg tagctgagca cagtctgctc gacgtcctcg atgtcgatgc gcggagcgga
     1381 ctcccccgcc gccgattcgc gctggaacac ggtgcgcagg tgcacgagca ccagcgtctc
     1441 ggtgcggttg tacggggcgt ccttcagcag gatcggcacc tcgaactcgt cggagcgcac
     1501 ctgctgcttg tacgccaggc ctcggccgag gtcgacgacc agccgcaggt acaggtcgtg
     1561 catgcgcgac tcgatgatct gctggtgctc cagcagcagc ttccactccg cgcggttgcc
     1621 ctcggcgctg aggaagcggc gctgcagcac gtgcacgagc acgcggcgga tctcggcgtc
     1681 gagcgtgccg cggtcgccgg ggaacagctc gtcgaggtcg tcctccatgg cgacgggcgc
     1741 gatgaacggg tcgtcgggga ggtcggcgcc ttcgtcttcg gcgctgccgg ggtcggcgct
     1801 gtcagcgcgg gaggtaatcg tcggttccga ggcctcggaa ccgacgatct cctcctcggc
     1861 tgcggtctcg cgggtggggg cggcgggtgc ggctgcatcc gcaccggcat cgatgcgacc
     1921 cgggtcggtc gtcgtctcag gcatcctgat cctccttggt cgcgtcgggc acggtggccg
     1981 cagagcgggc cgtcaccgcc ccgaacgcga atcgtcgcgt ggtgccgtcc ggacgcacgg
     2041 tgtcggcggt ggagatctcg tcgtgctcgg tcattccgtt gcggtgcgcg atctccagca
     2101 ggccgaccag gtcgacgggc cggcgggtgt cgtcgggggc gtgggcgaac gcctctccga
     2161 ggtcgaactc tgcgccgagc ccggcgacga acgcctccag ttcggcatag tacgggccgc
     2221 cccacgagcg ggaatcggcc tcgaggtact ccacgtcgtc ggcatccgtg agcggtgcgg
     2281 gtgcgcccgg aggacggacg tcgccgacgg tctgccgcag ccggccgagg tcgatcagcg
     2341 ggaggctgcg gacggggtcg acggatgccg atgacggcgc gtccttggac cactcgtgga
     2401 gtcctgcgat cacgtcgcgc agcagttcat cgatctgccg gtcgcgcaac gggtcgtggg
     2461 tcttcacctg accggtgatg acgtgggatg cccgccgctg cgccgccagc acctcttcga
     2521 cgccgagttc cactcggcgt ccgatcgcgt cgaactcgat gcgctgcgcc gggtcgagga
     2581 agcgggcgaa gggctgtgcg agcaggccgt gcaactgctc ggtgagggcg tcgatgcggt
     2641 cggcgtcgcc gatcagccgc agggcgccgg cgaaggcacg gccctccggc gtggactgca
     2701 tgacatcctt ggcgcgctgc aggtactcgc gcagcacgtc gccggtcggc cggtcgtctc
     2761 ggcgcaggtg ggagacgaca tcgcgctgca tctgcttgag cgactccgcg acgcgcgtga
     2821 agtcggccgg cagctcgcgg gcgaggtgca gcacgttctc ggcctcttcg tacagctcct
     2881 cgtcgtcgag gggcaccgcg ccgttcgcct ggatgttgag gatctcggcg tccagggcgt
     2941 cccgctcggc ggtgagccgg gcgatgcgct tgaccggatc ggcctcggcg tcgtccgaga
     3001 gccgctcgac ggcctcgagc agcgtgcgca cccgtgagtt ggagacgcgg gtgcgtccgc
     3061 cgccggtgcg cccggtgatc tccagtgcct cgacagcgtg cgcggagagc cggtagacct
     3121 ccacgtcgtc gtcgatctgc tgcaccagcc agccctgctt ggcccaggtg cggcacacct
     3181 cgcggccggt gccggccggg atgcggcggt cgtcatcgtc gtggccggcg gcccgcagct
     3241 cgtccagagc ctcggcgacc tcggcatggg catccgcgat cgggacggaa ggacggtcgg
     3301 cggagaagag catcgacagc gcggcgatca cgaagggcgc ctgacgcccg tgcagcagcc
     3361 ccagggtcgg cgttctgaac gctgccagag agcgggaata tgccgcctcg gcgcgcgtac
     3421 cagtcatcct cacgagttta tcggccccgg tggacgacgc cgttcacggc cgtcccgctc
     3481 acgggtgacg cgggcgggga tccttgagca ggtgcgcgta gccggcacgg tgatcgggga
     3541 acgcgaaccg gaatccggat gcccgcagca gggcgttcga gcagcgccga gctcccccgc
     3601 gcgtgcgctc cacctcgccg gcgggcggct cgggcatgcc gaggcggtcg gcgatgaacc
     3661 tcagcacatc gccgagctcg gcgggggcgt cgtcgacgcc gaggtacagc tgctcgggtc
     3721 tctccgcgcg cgtgagcagg tgcagcacgg cggccgcagc atcgtcgcgg tggatgcgat
     3781 tggtcaccgc cggggtctgc aggatcacgg ctcgcccctc gcgcacctga tcgatcagcc
     3841 gcgtgcggcc gggaccgtag atgccgccga gacgcagcac cacggcgtcc gtctcgctcc
     3901 ccgcgaagaa gtccaggaag cggcgctcgg cctcgagcag cacggcggcg cgtccctcgg
     3961 gcgccgcgat cggcgtggtc tcgtcgacgt cgccgggggt ggagccgtag acggcggtgg
     4021 atgacacgag cagggcacgc cggggcgcgg cgccggcggc atccatcgac tgcagcacgt
     4081 gctgcagccc ggtcaggtag gtgtcctcat agccgtcgtc gcggccgggc gcggcggcga
     4141 tcaccagaag gtcagcccct ggccattcgc agcccgcgac gatgtcgacg gcggcgaact
     4201 cgatgcccgt ctgcgggccg gcaggcgggt tgcggcgcca gccgatcacc cgctcccctg
     4261 ccgcgtgcaa ccggagcccg acctcggtgc cgaggtcacc acaaccgacg atgagggcag
     4321 tcatccgctg atgatcaggc tcgagatgag tccgtcgcgc agctggaaca ccatcggtcc
     4381 ggtcccgttg tatccgttgc ccgtgacggt cagggtgacg acgaacgcgc cggtcgcctc
     4441 gcccggttcg accgagacga gctcgaagtg cgactgcttg ccgatgttgt ccgtgcggtc
     4501 ccaggagcgc acgccgtcgc gaccgtggaa ctcgcggccc cagtcgtcga ggtaggcgtc
     4561 tgtggtgaac gcggcgacga acgcatcggt gtcgccgctg ttcgtcgcgt cgatgaacgc
     4621 ctggacggcg gagggaaggg ttgtcatgag atgctcctcg gtcggctgat ggtgacgctg
     4681 gtgacggtat tcacgccaga ccgccgttcg tgaagatgac ctggccgttc acccatcgcg
     4741 caggaccggc gaggaaggcg accgtctcgg cgatgtcatc cggctggccg aggcgctcga
     4801 ggggcacggc cttcgcgaga ccctgcacgg cctcatcgct cttgccctcg aggaacagcg
     4861 gcgtggcagt cggtccgggt gcgacggcgt tcaccgtgat gtcacggccg cgcagctcac
     4921 gcgccaggat cagcgagagg ctctccaccg ccgccttggt ggcgacatag gggccatagg
     4981 cagggaactg cgtgcgagtg acgctggtgg agaagttgat gatcgcgccg ccggggcgca
     5041 ctgtccgggc tgccagctgc gagacgacga acgtaccgcg caggttggtg cgcacgatgc
     5101 ggtcgaagtc gtcgagcgag tagtcggcga cggggccgag gatcatgacg cccgcggtgt
     5161 tcacgacgac gtcgacgcct ccgaaggcct gctcgacggc cgcgaaggcg gtcggcatgt
     5221 cctgctcgtc ggcgacgtcg ccgccgacgg cgacggcctg tccggcgttc ccggtgatct
     5281 cggcgacgac ggactcggcc ttggccttgt tgcccgcgta gtgcacggcg acggcgaagc
     5341 cgtcggccgc caggcgcagg gcgacggcct tgccgatgcc gccggagccg cccgtcacga
     5401 gggcgacgcg ggaggtctgg gtggtggtgc tcatagtgct tcttctctcc ttgtgggtct
     5461 gtgctcaggc gagtcgctgc agccattcag cgagcagact gcgctctgca ggtgtgaacg
     5521 tctcggatga gccgaggtgg gcggcgagcg tggtggccgc tgctgtgagg gggtcgccgg
     5581 gtgcggcatc ggtgatgagg gcgccgagca cggcatcccg cgtggcgacg gatgccgtgg
     5641 cctccgggta gctctcgggg cgcaggatga ggccgagtgc gacgccgctg ttcgccgcca
     5701 tcacgatgcg ggcggcttcg gcgggctcga cgcgcagccg gcctgcctcg gcgaggcggt
     5761 tcaggatgcc ctggagaagc cccatcgcct cggcgaacgc cgcagggcgg gtggccagcg
     5821 acgacgcgaa gacgaggcga taggcatgcg gatgccgcag cgcgaacgcc gtgtggttgt
     5881 cccaaccggt ccgcagatcg tccagcgggt cggacgaggg ctcggccgcc cgcttcatct
     5941 cgagatactc accccacacc cgatcggcga cggcggcgag caggccgtcc ttgtcaccga
     6001 agagccggta gagcacgggc tgggtgaccc cggccgcctc gcacaccgcg cgcgtggaga
     6061 tctcgcctga aatcgattgg gcgagcagct cggcggcctt ctcaagtatc actgttctcg
     6121 tcgtcacgta tcaatgatac cccagattga tatcaatgat acgagcatcg tggggaacgc
     6181 tcccgatacg acaaaacccc cggcttcacc gggggttttc atctgtagca ggagcggggc
     6241 ttgaacccgc gacctcacgt cgagcaagtg cgttctgcgc ctgtgcgaca ttgggcgaca
     6301 gtcgccccat ttatcccgac tgatcagggc tgaatctcga ccacacaccg acactgaccg
     6361 acatctagtg aagcgtgcgg aaagcgacat gtgagacgta tgtgagacgc tacgcgcccc
     6421 actcgccgat gtcgaagtca tcccccggcc cttcgtcggg ctccatccac gattcgtcta
     6481 caggctcccg ccgcatcacg cacgctgtcc tcgtgtccag cggcggcagg tcctcggccg
     6541 cgatgtagtc gatcatgtcg aagcctgtca cccggtgctc ggtgaccagt gggcgcccgt
     6601 ccttgcgcgt catgctgcag ccctcccccg ccgtccgacc atcgcacgga cacgtcgcgc
     6661 cgtgatcggg tcggcgcgga tgccgtcgat cgatctcgcc gcccgaatca gcagctggta
     6721 aaaccgggcc ggcgggatgc cgagctcagc gatgatcgcg gcttccttgc cgggcggatg
     6781 gccggggtgt cgtcgctcga acgcgagcag ggcggatggc gtcatgggct ctaccctgcc
     6841 gcctgccacc gacatacgct gtcgggatgg acgacgatct cgactgggga gcgcagcccc
     6901 gatgccccga ggacggcatc ctcatgcgag acgtcccggg cgggtgggag tgcccgcact
     6961 gcggtcacgt ccagcatgcc ccggaggtcg agctgccgcc cgagttcgac gggccatcca
     7021 tccacggtgg ctgacgtcgg cgcccggacg tagactcgtc tcctctcggc cggaggaagg
     7081 ggcaccggca ggtgggaacg aacaagcgat acgccgatgc gatcgaccgt cgagcgaacg
     7141 cgaagtacat cctcgaggtc tcgcgcaaag cgccgctgca gtcgctcacc gagaaggagc
     7201 tgcagatgga catctacccg ctgacgacgt atccgaagcc ggagcgctgc cgggtctggg
     7261 tgcgattcgg gcctcacccg ttcagggtcg aggggcgggt gctcaggtcg acgcagctcg
     7321 ccgccggtgt cgagttcacg ctcgaagaga ccacgttccg atgctgggtg tggggcccgg
     7381 cgtgcgaggt catcaccctc gacgacgtga tcgcagacag gcgggcgcac ggcggtctcg
     7441 cctgagcgtc ggcggcgtgg cgtagggttc ctgccatgtg cggacgattc gcgatggacg
     7501 ccaagaccga cgagctcatc cagcagttcg tcgccgacgg cgggaaacct gaggactggt
     7561 ggaaggactt ccacgggtcg tactcgatcg cgccgaccga tgatgcggtg atcgtccgcg
     7621 accgcggcga gggtcgcatc ctcgagctcg tgcggtggga ctggcagaag cccgccaaca
     7681 ggcccaaggg cgccccgatc atcaacgccc ggatcgagaa gctcgacacc gggttctggc
     7741 tccccgcgtt caccgccgca cgctgcgtgg tcccgatgcg tggctacttc gagtggacgg
     7801 gtgagaaggg cgacaagacc ccgcactacc tccacggtga cgggctgctc gccgcagccg
     7861 gtctcacctg gtcgatggag ctccccaacg gtgagcgctc gcgctgcatg gtcgtcgtca
     7921 cacgcgaagc gcgggacgcc ggcggcgagg tgcacgaccg gatgcccgca ttcctcaccg
     7981 ctgacgcgat ggacgcatgg ttggagccgg tgaagctgga ccgcggcgac cgcgacggga
     8041 tgctgtccat gctcgacgcg gcatccgccg aggtcgcgtc caccatcacc cagtacgtcg
     8101 tagaccggaa ggtgaacaac tcccgcacgg tcgacaccac cgacccgacc gtgatcgcac
     8161 ccgcggccta aactcaggac catgctcacc ggtcgtatcc gccccgtcgg aacccgcacc
     8221 ctcgaagtcg acgggaccca cgacgagatc gtcgccgcac tgcacgccgc ggcagtcgac
     8281 gggtgggaga tcaactcgat cacgacccga cgcctggtcc gctacggcga gacgactacg
     8341 gtcgaagccg cggacatcga cggcgtgcgc gcgaaggtgc cggacggtca ccagctgctc
     8401 agtgtcaccg cgagctgacc caacgacgaa accgccccac ctccgaagag gcggggcggt
     8461 tcgattgtct tgaatcaggc gtactcgaat gcctgagtcg ccgagtcgac gttcacgcca
     8521 ccaatgtgga aagtcccggt tcggcgggct ttcgagtttg cgcgcaagta gaacatgccc
     8581 atcgaaagct ccgcgttaac gccttcagaa tctgcttcca ctccgtctgg aacggcttca
     8641 atcaccatga ctttgaacct gcggacttca gccatgatga cgttcgctga cccgccgacc
     8701 gcatcgattc gcttggtgac atcgctgagg tactcaccag gagtgcacac aacgaccggc
     8761 cgtgatccgt ctgcagcgac gtagcgcagc ttcacggcgc tgtcagggcg catgatgctg
     8821 tgcatcaccc ggcgggttgc ttcgtggcga ttcatcgcaa ctcatccaat cccatggttc
     8881 gaaggtacgc gttgtagacc tcgtcatacg cgtcgtggaa cgattcttgc acgtcctgct
     8941 gtgaggatat cgctctgatc gccttgaagg gcgcattgtg atcgccgtcg tgacgatgaa
     9001 cgcaaccatg attcaaggtg tcaatgcata cgatctcgag cgagcgctcc cctatcttgc
     9061 gcgagagaac tacggcaaag gccaccaact gctcgttcca ggtcacacga cgcacggaca
     9121 gaacgtgagt ctcgtcgagg ccgacagacc accggtcctc gtcgcagtcg tcgagcgggg
     9181 gcggggccca agcggccact ttctgctccc tctcagacat gctgatgatc ataccgagct
     9241 atcgcggatg ctccgtcagt ccgacgtcca ccccatgcac taagccgccc caccctcccg
     9301 aaggaaggtg gggcggttcg cgtgtgcggg tcagcgacgc gggaaggtgt cgggtgactc
     9361 gtgcacggtg atcgtcggcg agatgcgctg ctcgagtgcg gatgcgggcg tcttcgtcac
     9421 catggagtac gagaactggc cgacgacgat cgtgagcaga acgagggccg gccactgcgg
     9481 cacgacgtcg cccgtgtagg cgtagtagaa ccacagcacc acggccgtga ggatgatcgc
     9541 gacgaggatc gcgaccgcct tcttctgcca cggcttgcgg atcgccttca ccgcgccatt
     9601 gatcagcgcg acagcgaacg gcgagacgag gccgagcagc acgagcacgc cggcggggat
     9661 agtggggaga gtgatgtcca tcaggactcc tatcggggaa ggccttccgg ccacggaggg
     9721 agggggatgt tgtgcgcggc catgtggtcg cgctggatgc cgatgaagga ccggtatgcc
     9781 tggttctcct cggtgagctt gtcgacgcga tcctcaagct tgtcgacgcg ctggtcgttc
     9841 tcatcgcgat agcgcttaag ctcttcctgc agctggtcga tgaagcggtc ttgggctgtc
     9901 gccgacgtct ccgcggaagc aacttccgcg gctgctgcgg cgacgtgtgc ttcacggcgt
     9961 gacttgcggt ccgctacacg gtgtccgatg agtacgccga tcaggatgca gaccgcgccc
    10021 gcgatcgtcg cgatgtcggc actctcgatc atgcccaccc ccaacatggg gtgcacatga
    10081 gcatgagcgc cgtgacgccg atggccagga gcacgccgag cccgatccac tcccgcagag
    10141 ggatcgggtg gcgacgcgcc tgcccgtgct gcacgttcgt cccgcgcttc acgacagcca
    10201 ccccgccggc ttcgcggcga gctcgccgaa cgccgtcgcg acctcggacc ccatgccggc
    10261 gaggtcgtcg cggagcttct cgagctcggt gcgcggcacg gtcaccatgt cgggttccgg
    10321 ctccggtgcg ggcgggttca cgatctgctt cgcccgctcc acgatccagt cgagacgcat
    10381 cgacgggccc gggcagatcg tgccgttcgt ggactccgcg aactcccggt gcccctttac
    10441 gtgctgccgg tccagcggga cggcgacctg cacgccgccg agcgacgtct gccgggacac
    10501 ccacgcgacg aactgcgcga tcacctcgtg ctgcgcatcc gagatccccc acgtcggggc
    10561 gtcggtcacg tcctgcacct cgatcgcgat gcagtactcg tcggccgcgc ccgtcgaagt
    10621 cggcttcagg tggtacggga caagctcgat ctcctcggcg cgggcgttga tgtacaggtt
    10681 cgggcaggag atgcggtcgt tctgcgacgt gaagaacgac tcctggtcga cgacgttccc
    10741 ggtgtggtgg atcgcgatgc cggtgatctt ctgcgagcga acgttcggct gcccgtcctt
    10801 gatccggtac gcgtacggcg gggtccatgc ccggttcgct tccgggtagg tgatctcgcg
    10861 cgcgacacgg gccgggagga ccggcttcga cggtgcgggc tcggtcgtct tcgggaacgc
    10921 cttcgcgtcc gacgccgggc cccagatcgc atccgcggtg aggccgttct tccgctgcca
    10981 cacgtagcag ccgaagctcg tcttcccgcc ccagatgttg tccgcgacga caccgatcac
    11041 tcgctggatg tccgacaccg ggcgcgactc gatgttgttg tggccggcga ggatcaggtc
    11101 agcgagggcg ttcgtcttcg gcccgtagac gccgtcatcc ttcagcccgc cagcgagctg
    11161 cagcaccttc gtcgcggcct gcgtgcccgg gccgtccttg ccgtcgttgt cgcccgagta
    11221 gaggccgagc agggcgagct tgcgctgcgc ctccctcacc cacgcctcac cgtagagctc
    11281 cctcgcgggc cagttgctgg acacggttcc tcctcctgtc ggggcggact gcccgacgac
    11341 gcggttgggg tcgagccacg ccatcgtgcc ggcgtaggtc tggttgtact tgttggtctc
    11401 gtgcaggtgt gggccggtcg accgggtgcc gagcgacccg acgaacccga tgtcctgccc
    11461 ctcgttgacg atctggccga cggagacgat ggtcggcttg cggtcctggt ggccgttgcc
    11521 gagcgtgcag ccgacgtcgt aggcgacggt cgtccaccac ccgccgagct cggtgtagtg
    11581 gatgctgacg accttgccgc gaccgagcgc cttgatcgcc ttcccgcgcg gctgcgcgaa
    11641 gtcgacaccg gagtgcccgg cgtaggtgct cggattgccg aacggcatgg ggagcttctg
    11701 gggcacgatg ggccctccaa tcgggtacgg cgaacgcccc gcgctgcggc gaggctcgac
    11761 agggttgggg tcaggatgcg accgcgatga cgatcacgcg gcggtcctgt tcgatgcacg
    11821 ccacgcgggc accgacgggg atcgatgaga ggacggactg aggggcgacg tcgaccgggt
    11881 ccccggcggc gtcgatgtcc ccgtcgaggt gcaccagcag cggggccgtc tgcgtgacgg
    11941 tgccccaccg gtaggtcgcc ttcttgtcga gggcggccca gagcttggcg atctgggtga
    12001 ggatcggctt caggaactcc ttcacgcgac ctcccgccac tcggcgctga tgtcggtgaa
    12061 cgggtcgaag ttcatcgaca tgcgctggat cgtcgccagg cgccggcgcc cgtcctcggg
    12121 ggtgaacgcg accagcgcat tcgggtcgag attgagcatc gcgtgggtga ccttcagccg
    12181 ggcgaccggc gccatcaggt cggcgagctt gcgggcggca agctggtcga gcaccgcctg
    12241 cgactcggct tccacggtct ccgcctccgg gccgatccat cgatggccgc gcgcctgaaa
    12301 tgagaacggg gacgcggggt cttcgttctt cgcggtcccg atcaggccct ccgagtcctc
    12361 gtcacctcgg gtgacgagcg tgaccttgtt cggcacgtcg gaaagattct gctcgcgatc
    12421 ccactccggg aagtgcagcg agtcctctcc gtgctggaac tcgaacgcca caggccgcga
    12481 tgcggggtcg gcgtaaggtt cgatccggaa ctgcccggac ccgtcgcacc acagcgacca
    12541 gtagccgatc gactgcagca gctcgttgat gatcttcagc ttcgacgtgc cggccgggta
    12601 cgtgacctcg gcggtgcgga ccttgtccga cggggtgacc gcgatccggg tctcacctgt
    12661 cgactgcagc agggcgacga ccgcggggat cacgggctcg ccgacggcga gggagaagcg
    12721 cgcgtcggtc gtgtcctccg cggggatgtt catcttcgtg agcagcccga cctcccaccc
    12781 gagcttgaac cgggtgtgct tctccacggg cgaggtcatc aggaacgtgc ccacaggcca
    12841 cgaggccgcg tcgctatgga aggtcgcccg gagccggtgc tccatgaagt cgatgccctg
    12901 gccgaggtca tcgagcagca gcttccccga cccgcccagc ggcgactggg cgacgatgtc
    12961 ggctgaccca ccgccgacag cgggactgac ctcgcgcagc ggtcggtcct gcgcgtcgag
    13021 gagatcgaac gaccatcgga gcattacgcc tcctcggcct cggtgagggt gaagctgtat
    13081 ccccagaagc cgttccagga gcgcatgccg tcgtcgtcgc gagcggtcgt ctggcgtggc
    13141 atgttgaccg gtccgatcgt gccgtagatg cggcggccgt caggatcacg gaagaggaag
    13201 atcggctccg gcgacagcgc catctgcagc agctggtccg gcgtcacggt gtcctggatg
    13261 aacttccggt cggtgacgcg accggagacg gccacggaac gggtggtcat ctcgccgcgg
    13321 tacgcgaccg gcttgtcccg gcctgcgtac tccttcagcg cgccgtctcg gctggtcgtg
    13381 tgggtgacgt ccgggttcag cggcagccgg gcggtctgcc cgaaccccac gccaccggac
    13441 agccagaggg caagggaccg ggcctccaca gtgatcgtcg tgacggcagc ggcgccctcc
    13501 gtgttcactg cgatcgcacg gtagaggatg tcggcgtacg acgcggactg ccagtccatg
    13561 aacgcggccc ccacctccgc cggcgtctct ccgagaacgg gggtccacgt gctgccccca
    13621 tcgtctgacc tctcgacggt gaccgtggac ggcgtcgcat ccgtatcggt gaacgcgatc
    13681 cgtccatcga cgatgaacac ctttccgtcg tcggcgcccg tctcgaagta cgggtgacca
    13741 tcgtcgaatc cgatgttggt ggccatggcg gttaccctcc ctcgggcgga tggggacgac
    13801 tcggcccgcc tgagagcggc gggccgaggt tctgctttcg ggtcagactg cgcaatatgg
    13861 catcctgagt cggtgggtgg gcggattgaa tctctagacg ggttgcgcgg tgtcgcagcg
    13921 ttgagcgtgc ttatcgggca cgcgatgtgg gtgacgcctg cattcgcgga tgccgtccag
    13981 ctttcaaacg cgggcgaagc agacttcatt actgccttgt tcacgtacac gccgctgcac
    14041 ctcgcatggc ttgcacgcga agcggtgatc gtgttctttg tgctgtctgg attcgttctc
    14101 gcgcgaccgt tcctcagtcg tgggcgcagc tacggcgcct actacccgca gcgggtcatc
    14161 cgactcgcgg tgccagcgtg ggcggctctt gccgttgccg cgctcatcgc cgtgatgctg
    14221 tacccgacga ccggcacgcc acattcggag cttcttgcgt cgcaggtcgc tccccatgtc
    14281 gagtgggggg acttcgccgg cgcgtggctc atcggtggcg caccccaggt gcttctccca
    14341 ctgtggtcgc tcaagtggga gatcgccttc tctctgctgc tcccgctgta cgtgctgatt
    14401 gctcggtggg cggtcggcgt gtggaagggt ggcgcggtcg cggttgtctg cctcggcctg
    14461 tccgccgcgg gaacgtattt cagcgttgac gagctcgcgt acctgccgac gttcatgctc
    14521 ggatgtctcg tcgcgtcgaa ccagcaggcg atcactcgcc gcatccgtga cagccgtgcg
    14581 acgtggctcg gtgtcggttc cctcatcgcc ctagctgcgg gatggtatcc gcgcggggtg
    14641 agcgattcga ccatgcttac cggcgcgggg caggtgctgt ccatcgtggg cgcagcgggt
    14701 gtcgtgatcg cggcgctcac gtggcgcggg ttcgcgcacg cactgtccgg gtcacgtgcg
    14761 atgctgtggc taggggatcg atcattcagc ctgtatctcg tccacttccc agtgatcatg
    14821 gtggcggcgt tcctgacgtt gggcgagaac ctggcgatca ccctcggcat cggcttgccg
    14881 gtgagcctgc tggtcgctga ggtgttcttt cggactgtcg agaaaccgtc gacggatctc
    14941 gcccgcatgg tgaagcgacg cctcgcctcg aagagcagcg cggcggaagc cgaagccccc
    15001 gccgcgcagc gtcaaagctg agtcgcctcc atgtcccgga cccacagagt gcctgtggaa
    15061 ttcgttgcac agaaccgaat ctgacagaac ccagccccgc gtggcggcat caccaagagg
    15121 tcacccgtca accagtcccg agtcacatcg gtcaggttgt tgtaggagaa tcgcagcgcc
    15181 tccgggtacc cccgctcacc cccaagcgac gtcgcgatgt tcgccgccga ataccactca
    15241 accaggtact caagggctga gcctgtggtg gtcaggtgtt cgcatgccgt cgccaatgag
    15301 aacaacacag ggcgccgatc gtccgggact cggaaccgct tactgaggat tgtcgttcga
    15361 ccggtcgccg cgagatcgaa cttcgccgcc cccaccgtcc cgtcacggcc gaccttcgct
    15421 gcgtcgaatg tgtaggccga tgacatcccc gacacgaacg agtcgtccga gatagtcggt
    15481 gcccccgtgg tgagcgtcag cgccgttgac agtgagctat tgcgcaccag agacgactcg
    15541 acgcccatcg agatccgcat tccgttctcg accatgccta tcaccgggcg catggtgatc
    15601 atgttccgat caggctgggt tgaggtgtcg cgcagcatcc gcatgccgag cgaggaacgg
    15661 tcgacgacgc tggtgccgtc aacgcttacc acgacggact catccggctt cactgtcgta
    15721 gggtctatac gtacgagcag gtgacacacg cccgtgttga cgaccttcgg ctgaacgatc
    15781 accgcgccgc cacggatcag caaggctgat ggccgacttc cgccagcctt gatggtgaac
    15841 catgcgccgg aatagcggcc ttccatgtgg cacgatgcga cctcaatcga cccaccctgt
    15901 atctccgcga tctgcgcgcc gaagtcgagc gaacatcccc gcaggaacat cgatgacccg
    15961 ttctcgggct cgaggaagat gaacggcgca ttggtgttgt tctgaaaggt gcagttcagg
    16021 aagttgaagt tctcccccat gttggtcccc gccaggcgta ccgaatttct caccgccgtg
    16081 tcgaaggtga cgccttcgaa tgaccatagg taggagttcg tctttagtga gacctggtcc
    16141 cggaacccac taatccgcac acgccggaac tctgcccatt tcgtgtcgaa atcggtcagg
    16201 tgtgagatgc catcaacagt agtcccgata ccggttggcc cgctgatcga taggtcggcc
    16261 ataaatgtgc gaggcagggt cgcgtcagca ccagtcgggt ccatcagtat cgcagggcca
    16321 ctggtcgcgc ctgtaaagtc cagactcgtt gactgggtgc cgatgatcga gtgaagtcgg
    16381 tgcttccacc gcacttgaga ttgcaccttg atcttcccag gcggcagcga cagcgccagc
    16441 ctcgaatctg ctgcggcgtc cagcgctgct tgcaacgcga ccgtgttgtc cgctgttgtc
    16501 ccggcggcaa caccgaagcg gcgcgcatca atagtctgcg tggcccgctc gatattgagc
    16561 ccgatctgcg ctgctagcgc ggagccagta tctgcagcct catctgccat cgtggccgaa
    16621 agctgagtag ccgtcaggga ccccgggtcg gcgatgaact ccgccactcc actgtcggtg
    16681 acggagggcc ccatcgcggt cccgaagtcg actgggtcgc cgttgtcgcg cacggtcttc
    16741 aggtgaccgt ccgcagggtc gatgtgcatg ccgaggaact cgtcctcggt gatcgcgtac
    16801 tttcggacca tcgttcaagc tcccgcttct acgtaccagg cgtcgccgtc caacacggcg
    16861 accgccgact catcaccagc gagcgtcaac gcgaccccgc cttgcatgtc atcccagacc
    16921 ccggacagct ccggaggctc cggtggcacg aacgccacca tgaacgtctc tgtgccgaag
    16981 atggaccaga ccccgcccgt cgcacccatg acgcggacgg tccactgccc ttcggtcaga
    17041 cgcttcgcca gcacataact gctggtcggc ccggaaccgt cgcgcgactc caccacctga
    17101 ccggtggcgt ccagcagctc cacccgccaa gcagactgcg gccgaccctg cgcctggaac
    17161 caggaccact ccacctcgag ggtggacgcg ggccagacat ccgcgggctg ggtcaccgcg
    17221 acaccgggcc ggtcgatcac cgtcaccgtc gccacggcag accagtctga gaaggatggg
    17281 tcagtgccct tggttcgcac ctgccactcg aacccgcccg tcggcagggt cacggaagtg
    17341 tccgaggcag tggttccgga gaccgtcgtc caagccccgc cagacgggcg ccattgcagt
    17401 tcgaacgccg actgggtcga gctatccaca gggttgtgcg tccaggacag caccaccggg
    17461 acatccgacg ccacgacccc accattcggc gccaggctca caggtggatt cggaggggca
    17521 atcagctgca ccgtgttcga cgccggtgac cacggagacg cgagagaccc gcgtacagcc
    17581 cgcaccttgt aggtgtgcgg ggtgccaggg tccggcgcaa cgtcacgcca cgggagggtc
    17641 acgttcgagg cgacgacggt cgcgccgtcc tgcacgtcga cgccggtcgc gatcgcggag
    17701 aaagatcccg agatgacgat gtccatgccg tcgcgcgccg cggacggcgc ggccggcacc
    17761 gacggggtgg tgtagatcgt tgccgtgctg gaccagctgg actggcccgc tcccgcgacg
    17821 acggcccccc ggtattcgta ccggtggttt ccggtcgtcg tggtgtcggt gaacgtcttc
    17881 gcgttcccgc tcggcgcgcc gacctgctgc cacgccccac cgtcggtgct gcgctggacc
    17941 actgccgacg tgttcaccga cgccgacgcc cagttgagcg tctgctgggt atccgacacg
    18001 cgcgtcaccg tcagcccggt cggggtcttc gggatcgtcg ggaggaccag gaccgcggtg
    18061 tgcgactcgt tgatcgtccc gtagtccagg gtcatgcgca cggtcaccga gcgggtgcca
    18121 tcggcgttgt gcgaggcggt caggtagtac gcgccgtagt tccagcccgt catgcccgag
    18181 gggaggaacg ggcttgcgcc gtaccgcgcg aactccccca ccccgtcgat gctcgcgatc
    18241 tgcaggccat cgccgttgaa gaagctgccg gagcccttcg acatctgcag gtacgcccac
    18301 atcgtggacg agttcccgcc gatgttcgtg gaggaccgat ccgccggcac cgtgaacgtc
    18361 acgtacgggc tggacttcga gacctgaatc gtgaccacga gcaacccctt ccgggcatag
    18421 gtgaggggtg aggcccgaag gccccacccc tcacgatctg ttacacggac gtcttgcgaa
    18481 cgtcaccgat ccgacgccac gtgaacacga ccgtcgcccg cggatggtca cgccgccact
    18541 cgagcacgta gcccgccgca tgcgaccgct ccaccagctc gctctgaatc tcccggcgag
    18601 aggccgcaga cagcaccatc agatcgagat ccgtgatctc gaagctgccg ctgctctgca
    18661 ctccctcatc ccacgccgct gctgctgcga cggctgacgg gatcatctgg aacttcgaca
    18721 tggtgtctcc tctagccgaa cggcaggtcg gaacgaacgg actccgcgct ggggaaagct
    18781 cctgcgacgc gggcatccgc gatgtccgcg acctgggcga tcaggtagtc gccggtgaac
    18841 gggctctgca cgtacacgac gagctgctgc ccaccgggtc ccgccgcgcc gacgacaccg
    18901 ccgtcggcca tcgggaggac accgaaggaa cgcatcgctt cgagcagcac cgacatcgac
    18961 tgtgcggatc cgtcgtccgg gatgtagaac tcccggtccc gcatccgatc gccgacgaca
    19021 cgccacgtgt tcggcggcac ggcctgcgcg cgaggcgcca tcggcgtcag agacccgccg
    19081 ctcgccatgg gcatcacgat gccgccgtca gcttgcgcgg aaatcgtctt gccgccgatc
    19141 ttgtacgact ggcgtccctc ggcgtcgacg tagaccttca cgaccttgcc gttgttcaga
    19201 ctgatgaacc ggtcgatctt gccctgtgcc ccggccgtgt cgacggagat cgtcgagctt
    19261 ccgtcgggga gggtgatgat cttggtgccg aggtcgatga ccttgccctt ctcggcgtcg
    19321 gcgttcgact tgaactcggt ctccatctgc tccggcgtct tcagcaccgt gtcgatcagg
    19381 ctctgcgcct gctcctgggt gagtcccatc gcgaccatct ggtcgagcag tgcttgccgg
    19441 gtggtgttgt agcggtcggt gaggttctgc tgcgactcgc cggcttcgcg cgcgaggccg
    19501 atctcgtcct cgaacgcctg gcctgcagct tcgagctgcg ccttcagctt cgcgccggac
    19561 tccgacgctg ccaggaacga ggtgtccact ccgcccagcg acagcttcgt gccatccgcc
    19621 gcgacgccga cccgcgtgat cgcatccacc gttcgcatcg acgcgtcgct caggtagccg
    19681 agcgcgacct gcccgtccgc gaagccgttg aacttgcccc gcatcttctc gaggctgtcg
    19741 ttcacgacgt cggtggagtc gctgaacgtc tccatctcgt tggcgaacgc ctcgatcttc
    19801 gtgccggcgt cgtggtcgaa gaagccgatc acgctcgaca ggcccttgac catcgccgcc
    19861 agaggcccgg agacgaactc accgaagccg gtgttggcgg tgatcgcgaa gtcgatggcg
    19921 ccgttgatca ggtcggagaa gaactgcagc acgggcccgc ggttcttcga gacccagtca
    19981 gcgaagtcgc cgagcggatc cgcgaacgcg acagcgagcg cacccttgat gccgtcggct
    20041 gcggtctcga tgctgcgctg cgccttctct accttcgccg cggtgttgtc gccgagctgg
    20101 tcgaacatgc gctgcgcgga tccggcgacg ccgtcgagct ggtcaacggc gttggacaag
    20161 tccatcgcga acagggcgtc gccgaggtcc tccgcctgcg tgccgaacag cgcgacggcc
    20221 gccgcgttcc gcttcaccgg gtcctcagtc tcacggagct tgtcgagcac gagagccagc
    20281 ccgttgcggg catcctctcc accgcgagcg atctgcgcgg tcatgtcctg agcgttcagg
    20341 ccgagggcct tgaagccggc tgccgacgac acagaagcat cggtcgcgcg gatctggaac
    20401 tccttcagcg catccgccgc gaggtccgag ttccgggcgc cggcctgcat gccctggttg
    20461 atcagcccga gcgcttcctc accagagagg cccaggcgct tgaacagcga cgggtactcg
    20521 gtgagggtgt cgatcaggtc ctcgttgcgg ttcagcccgt tccgggcgcc cgccgcgatg
    20581 atgtcgtacg cgccctgcgc gttcttcgcc aggccggtgg agagcagctg ggtgacagcc
    20641 tgcgcaacgg ggcgcacatc ctcgcccagc acgtcggcga tgccggcgag tccctggacg
    20701 accagctgtg catcccgggt cgtggccttc gggtcgagga tccggaactg cagcgcgagg
    20761 cgcgcggtgt ccatgttcgt ctcgatcgac tcgccgaagt tgttcgcgta cgcctcgccg
    20821 gccgcgcggc cgagtcggag cgcgtccgcg gcgctgatgc cggtgagggc ctgcagccgg
    20881 tcctgccggg cgtcgacttg cagtccgtcg ttgatcgcgc cggtgatcgc cttgccgatc
    20941 gcgacaccgg cgaggatgat ccctccggcg atcgggatcg cggacagcgc gtcgacgagc
    21001 gtgttctcga tgtcgccgcc gacgacctcg ccgaccttct cccccgcgcc gcgcgtcgcg
    21061 gcatccaggt tcttcgacag ggagcgaccg ccttcgtcgc cagcttcctc cgacttgcgc
    21121 ttgaacaggg agaccagtcg cttcagaccg gactcggcct tctgctcgtc gacctcgacc
    21181 tggatctcgt tcttcgcctt gacgagccca tcgagcgtgc gctgggtccg cgcgagctgc
    21241 gcctcggcgc gcttcacatc ggcaccgacg tcgagcccgc cgagggcgcg cacctgaagg
    21301 tcttccaggc gctgcttcgt ccgttcgagg ctcttctccg cgcgggagat atccgcgtcg
    21361 agcttcaggg cggtgtcctt cgacacgagc ttcctggccg cgtcctcgac acggtccatg
    21421 ctgtcgagcg cgggcttggc gttcgcgtcg acctggatgg gcttggactc gaccttcttg
    21481 ccgaccgcga cgatctgctt ctcggccttc tccacctgcg tggtgttcgc ggtgaagagc
    21541 acctcgagat cggcgacggt cagcttggcc atgggtcacc tccgggtgag cgcctgcctc
    21601 agtcgcgatg gcgagtcgag cagagcgaag atcatcgtcc gcacgccggg ccacggccgg
    21661 cgcctgacgt cggggtcgta gatgtcgatg tggaactcgc gcgcgagctc cgcgacgacg
    21721 atccgccagt gctgcacgat cgccagccag gacgtgtcga tctgagactc gtcgctgggg
    21781 gcagcgggcc cgtcgtgggc cggcttgagc ttgatgggga ccggccgata gtccgggtac
    21841 cacccgtcgg catcgggttc accgatgccg tagggcgccc agtcttcggc ggtcacgagc
    21901 cttttggggc tgcctccgcc tcccccggct catccatgtc gcgcggcgcg aacagcaaga
    21961 cggcgagctc gtcggcgtac tccttgccgc gggtccagta gaagaccgcg tagtacgcca
    22021 tcttgtcgat cgtccagggg tcgattccgt cgtcgacgag ctgctggtac gtcgcgccga
    22081 gcgcggggtg ttccttcggc ccgatggtgt cgaggatccc ctgcacctcg tcggggatct
    22141 ccccctcgac gatgccgagg ttcacctcgc cgcggacggc gcacgcgagc aacttcccca
    22201 tggcgtccac gctgggcggg cgaaccgtgt aagtccgctc gcccagcggg agaacgaggt
    22261 cgggcaccgc ccacgccgcg aagtcaggtg cggtacccac ctggtcaggc tcctcgcgtg
    22321 taggtgaacg cgggcgatgc gccgcccggg gtggtgatga tgaccggcac ggcaccggcg
    22381 gtgtcggtcg gcacgagcgc gacgatcgtc gacccgttga tgacctggaa gtcgtccgac
    22441 gggatcgcgt cgaacgtgat ggacgacgca ccgagcaggc ccgaaccgtt gatcgtgacc
    22501 aggtctccgg atgcggcgcc cgacgggctg atgccgctga tcgccggggc cgtgacatcc
    22561 cagccctgga acgggttcgg gatctgctcg aactcgccct tgccggtcag cgtgatcgcc
    22621 ttcgtctcga tgcccgtgtt gtcggtgttc gatcgggtga tgtcgacggt gacgaagccg
    22681 cgcccggcgt cggtcgggtg aggcgtgccg atctccggct tgtggtagta gcggacgtcg
    22741 agcacggcgc cctcgccctt ggccttcgcg gcggcgacca tggcctcgag ttcgggcagg
    22801 tagagaccgg tggtcagcga ccggttaccc tgcacggtga gggacgcgtt gaacccgcga
    22861 ccggtgacgt cctcgttcgg cgacccgagg tcgtcgtagg tggcggcatc cgtggtgatc
    22921 ggcggggtcg acggctgcca tgcgctcatg cggcgcacgc tctgccaggt gggatcggcg
    22981 taggtgccga ggttgacgtc gagtccgtac tcgtaggact tccccagcac ggtgccggcg
    23041 gggagagtga ccttgttgct catgatgagg cctccaagtt gtcgagagtg atgaggtagt
    23101 tctcggtccg ctcctcgcgg ccgttgtcgt ctgcacccag cgatgccgcc gaggtgcgaa
    23161 agatgctggc catgccgtcc ccgcgggggc gttcatgcag gacagcgaac aggacatctg
    23221 cgatccggtc ggcaccgaaa ggctggtggc ggcggccgcg gacatagagc tgaacccggc
    23281 gggagagcag gttgggttcg tcgatcgggc tgtacacgcg gatgccgaca gcccggtcgg
    23341 gcgcgtccgg gatcgtgccg tagaagacgc cgatctcggt gtcggtgtag ttgggcccgt
    23401 agggtcgcca tgcgaacgag gggaccgcgc cgacgatctc gcagaggcgg gtcgtgaggg
    23461 tctcgtcatc catcgagcgc cgccttcact gcatcggcga cgatgcttgc gaggtcgacg
    23521 tcgtcggatg cgcgctcgag gaacttcgcg ccgccgtcgt cgtgctgcca gtcgagatgc
    23581 tcgtgctgga agaccgcatg cggggcggtg tacctgaccg tcacggagag gtcgtcgacg
    23641 acgaccttcc ccgacgtgat caggtcgccg tcgtcgcgtg gcgccagctc gttcgaccgc
    23701 ttcagcagcg tccggccgcc cttgcgcatc gcgtcctggg cggccttctc gaccatgctc
    23761 agcaccggct tgagttcgac catgacggcc tccctacttc agccagagaa tctgatggga
    23821 cgggagcggt ggcgggttca cgtcccgccc gatgcgcagc acttcggctt cacgttcagc
    23881 gcccatcacc gcggaccaca cggttaccag agagccgagc atcacgttcg cctcgagatg
    23941 cacggtgact cgggtggagg agatgacttc ctcgccggcg gcattgcgca cgatctcctg
    24001 cacgtcgatg acctcggccg ccagcgtgcg cgtgttcccg tagccgcctc ccatgccgcc
    24061 accggcttgg tagtcgcgga ccttcaccgt gtccgggaag aagaacgact ctggccaggt
    24121 catcggtacg tctccggcca cagcactgac gcggggcgct cggtcgggaa gcttccggcc
    24181 ggaacacctc cgcccgccgt cgacgtgacc ccgcagagcg actgcaacgc ctggcgggcg
    24241 tcatcgtcga acacggtggt gatgtccgcg aatgtcatcg atgtgccgtt gcggctgagc
    24301 gaccgggtgc gtctcgatcc cgcggcgggg acctcgtcga ggaggccctt caggatcgcg
    24361 atcgcgtcct tcttggcttc gtcttcgagc gagtcgatgc agggggcgat ggatcgtgcc
    24421 agcacgagga tccgccgtgc ggtgttctcg tccccgccga gctcatctgg tgtgatcgcc
    24481 atgatccatc gccccctact ccttaggcct ctgcgatcag accgaccgcg atggcgtgct
    24541 tgatgctgtc cttcgaccag gcagcggggt cgacgggtgc gccgcggtac aggtaccgct
    24601 cgcttccgtc ctcggatcgg agaacgaccg cggcaccgac gaccttgaag tccttcgact
    24661 tcttggtgct ggccgttgcc ttggcgtccg ccgccgcctt gtctgcggca gccttggtcg
    24721 ccgcatcctg cttggccttc tcgtctgccg cctgcttctc gtccgccgcc tcatcggcgg
    24781 cggccttctg ctcggcagcc atcagagacc cgttcccgtc agctgaacgc cggcgagagg
    24841 ctcggtgacg acggggaccg tgacgcgacg accgcgcagc gtgtagctgt cgtcgtcgtc
    24901 gttgcggatc gacttcgcct cgatgccgac ctggcccgcg ccgacgtaac cgggaccgcc
    24961 caggggctca tcggccatgc cgccgagctg ctcacggtcg accagcagcg ggttggcgcc
    25021 ggtgtagttc ggcgacgcag cccaggtcag gccgagaacg tcgacgggga tgttgccctg
    25081 cacggccgtc gcaccggact cgcggggcag tgccttgtcg tcgatcagga ggccgatgac
    25141 cttggcgtac tgcgccggcg tcaggacgat cgtcgagagg tcgatgccga ggccgagcgc
    25201 cgcgcgggtc gcctggatcg acatgatggc ctcgaccgcc ttgcccgcgg tcgtccaagt
    25261 ggacggcgag gcgaaggtgc tggtcacctt cgcggcgatg acggccatcg cgacggagtc
    25321 gacgtggcgg acgatcgtgt tgccgagacg ggtgagcccc ttgttcacga acgagatgcc
    25381 ctgccgcttg atcttctcgt ccgagatgac cgtgtccagg ccccacttga cggtgcgggc
    25441 cgagacgacc tcgcccgact cgaggaccac cttggggtac tcgccgagcg gcgcgatcga
    25501 ctccgggtcg tcggacgcga aggcgtcctc accggtttcg tagaagacgc cgccgccgga
    25561 cgcgtcgaag cgcccctgca gcaggaagtc cgcgatgaac ttcatctgcg tcaggtcgtg
    25621 cagacgcttc gcgatgacgc gagggttggc gagcagcgtg tgcagctgct ccgtggtgag
    25681 cgtcccctcg gggtgcttca ccgggtaagt gtacgaagcc atgtgctcgt cactcctctc
    25741 agatgaacag gacgtcaatg acgtcgttgt cggcggccgc ggcggtgagc gcgagaccga
    25801 tggggttggt ggtcgagccg accgtctgga ccttgccggt cgcggccgcg gagaccttca
    25861 cgccggcgac gatcgcgccg gacgcggtca gcgagtgcac gccgccagcg cgggtgaaca
    25921 cggtgacgtc ctcgccgatc gcggcatcga acccggcgac gccgacgacg gccgccgagt
    25981 cggcgcccgc ggggccgacg gtgcgtgcac cggtgatcgc gacgaggcga cctccggtga
    26041 cggccgctga ggccttgaag gtgaccgcct gaccgggctt gaacttgggg atgtagtcag
    26101 ccatctcagg cctcctcgtt ctcgtcgccc cagcccgcct gggccatgag cgcgtcttcc
    26161 gccgacacct cgccggcggt gtgtccgatc tcggcgaccg ggacggtgtt cttcgccagc
    26221 gagttgatcg tcgccgtggc ggcggtctcg tcggcgtcga gcatggcgcg gaagctcggc
    26281 gcggacgccg gggtgattcg gccctcgctc agcgccgttg cgatgatgcc gtcgcgacgc
    26341 tcgttcacct gctgtgcgcg tgcctcgaca ccggcctgcg cgttctcgcg cagctcggcc
    26401 agcacgtcgt cggcgatcag agtcgtgccc gcggggatgg tggtggcggc tgccaccggg
    26461 gcttcggcct gctcctcgag cgcctcgtcg agagcggcga tgaccgcctc gtcggtggcg
    26521 tcggcgtcgg tcatgccgag ccgctcgcga atgccagcct tgaagtcgtc gtaagccacg
    26581 acgttctcct ttcggttggg gtcacccggc tcggacgagc tcgggagttt cggggcggcc
    26641 gctgccgcca tggtggggtg ctgcgcgaac cggatgactc gcgcggaggc cgagtcctcg
    26701 ggctcctcct cgtcgggcac gacgacgatc acgtcgtcat cgccgacagt tgctgcttcc
    26761 ccggcgtcgg ggatgaccgc gatgcggtca gcgagtcctt cgctgaccgc atctgctgcg
    26821 gtcagccagg tttcggcctc gagcagactc gcccagtcct tctccccggc cttggaggcg
    26881 tagatctcgg cgagtgaccg gtccagacct tcgagcacgg cggcttcctt gcgcatgtcc
    26941 gccgcgttgc cccagacgat ggtcgaggtg cagtggatca tcatctgcgt gccgggcgac
    27001 atcaccgtct cgtccgcgcc cgcagcgatc accgacgctg cggatgccgc gaggccgtcg
    27061 acgacggcgg tcaccttcgc gcgatgtgct cggagcaggt tcatgatcga gatgccctcg
    27121 aacacgtgcc cgcccgggga gttgatccgg agaatgatct gcgacacaga ctcggggagc
    27181 gcatccagca cgccgccgac gtccttcgcc gagatccccc agaacccgcc ccacgagtcg
    27241 atcggcccgt acaggcggat cgtcgcgacc tcgggctcgt cgccgacggg cggcggcgcg
    27301 acgacagcgt cgaagaactc gcgcttcgtc ttcggtaggt cgtgactccc ccagaatcgg
    27361 ttcggccacg tctctgcgcg tgtcatgcct gctccttcag atcgacgccc gtgcgggcgg
    27421 tggccgggtc gggcgcggga aggcccgcgc tcgcgcggat gtgcttctcg agcgtctcgt
    27481 ccggggtgat gaccttcgcg tcgacgagtg ctcggatgct ctctgcgacg agcgggtggt
    27541 ctttcccgag tcgcgacggc gagatgatcg gcgcgagctc gtccggcccc cagttgatgt
    27601 cgaccaggtc ctcgacgacg tgctgctgca cgacgtccgc ctgatgctcc atgactgcgt
    27661 tgagcgagcc ggtgaagaag tccgcgaacg tgtcgccgag cgcgtacgac ccggtggagt
    27721 cgtcgccgcc gagggtgagg aagtgagcga gcactgcacg agcgatctgc tcgtcgtggt
    27781 acttgatcgg ccccgccatg tcgggcagct tcccggtgac accgatcagc gacagctcgg
    27841 caccgtgggg gatgtatgcg ccggccgctt cccccgcccg gaactcagtc gtaagcttca
    27901 ggccttcgtc gcgctgtgat tccagccact tctcggcctt ggtggcgtcc tttgagacag
    27961 catccggtag tggcgccccc ttgtagacgg gaatgccgag accgttgcgt tctgcgacga
    28021 ggagctcgat gcgcaggaac ttgtccttca gcgcccagtc cttgtacgcc tgtcgcagca
    28081 gggatgtgcc gagccagttc gcgccctcgc gctcgttgac gaagacgact agccggtcga
    28141 ccgggatgcg caccttcccg gagcccgaga acccgtactg ctcgatcgcg ataagcccgc
    28201 cgtccttcgc gacgtcgaac ttcgagatcg tgcggggtgg gcgccatgcc agcttcccga
    28261 gtcgggcgcg cccgttctcg atgcggtaca cctgctcgaa aaccgagtgc ccgaacggga
    28321 gctcgagcag cgcgagacgc aggaactcgt cccaagagaa tcggcctcgg ccgcgcttcg
    28381 gtcgaggtgc ggactgctga cccttgatcg gcaggccgag gtcctcggct acgagctgca
    28441 cgacctcgtc gcgcgcgccg gcaggatcga tctcccaccg tgtcgagcgg atggggagcg
    28501 tcactgctcg gagcacggag ccgcactgag cgtcctcgcg gcgcatctga tcgaagatct
    28561 cgatcgaata cggccactgc agcagtgggt tcttctcatg tgcctcggcg gtcaggtcgc
    28621 cccatccagg aagcgcgtca gtcacgtatc cgatctcgtc cggcatcggc gtgcctcctt
    28681 tcagaactgc atggtccgtg cgttcggctc cgtcgatgcc accgtgctgc gcggtacggt
    28741 gacggcgggc ggaggcttca cgggctccgg ctccggctcc ggctcgagca cctcgagcgc
    28801 gtacagggcg atggtcatcg cgcagacacc ggcgatgtcg accggcatgg acttctcacg
    28861 cgaccagccg atgttctcgg cgtaggaccg gatgacggcg ccttcgatcg cgcggtcgac
    28921 gtcaggttgc tcgatgagca gcaggtctcc gtcacgcact cggtcactta tgcggcctgt
    28981 ggcgagcgcg aatgcagacc actcgacctg gtgcacgatg aacttctcgg ttcgcccatc
    29041 gacctcgaac tcggcatcct gcagcggctt gatgaagtcc atcgccggga cgcccttcga
    29101 ttgcagcgcc acctcgcggt gaccggactg ctgcgcgagc tcgatcagga acgggaccac
    29161 ccacgcccag ccgggccgct tcacccggat ggtcgcgaac gggcggccgt cctcggtgta
    29221 caccgcggcg gcgagccagt tgcgctgtcg gtcatggctg gtgtcgatcg cccagaccgt
    29281 ccgagcaccc ttcgggatca tcgccaacac atcagccggc gtgacatggc gggatttcca
    29341 cgtcgccgtg tcgatgtagt tgtcgaccgt cgcggtgacc cactgtccga gcacctcgat
    29401 gcgctcgacg ttcttctccg gggcttccgc tgctgcacgc atcagcccgg cgatcgtcat
    29461 gccaggcagg tacccggcgg acgggttcgc ctgagcgaac gcccgggggt cgtcgagcgg
    29521 cgcgtcctgc tccgccgacc actccgcgac gaaccactgc gtttctgggt cgtctacacc
    29581 ggcgtgcgcg gtctcacgga cgcccttgag caccttcgag cgcttcgttc ccgcgttcga
    29641 gaaggcgacc agcagagagt cgtacatcgc gttcgcggac ttctcgatcg cagaccagcc
    29701 ctcgtagtcg tactgctcgc gcagctcgtc gaggatcaac cgggcggatg actggccgcg
    29761 cgcaccgtcg aaggtgcgtg gaaggtaggc ggcgcccgca tgcgtccgaa gttcggtctc
    29821 gccgttggtc gttcggggcg ggtaggtgat ctcctgcagg tccggcacgc ggtcgatcgc
    29881 gatgccgacc ttcagatcgt caggcgccgc ccaccgacgc acctgtttcc acggcttcat
    29941 cgcgatgtcg agcttctgcg cggcaccgac gacaatgaag ttctgctcgg gaagctggtt
    30001 cggccaccgc cccgcatcga cgtacaacca gtacgcggcg agcaccgcgg ccatgagcgt
    30061 cttgccgttc tggcgtccga cgatgacgag cgccttccgg aagcgcagca gcccgaatgc
    30121 gtcgagctcg agcatccgga tcagcagcgc ccgctcccac ggaaacagca ccacgttcag
    30181 cacgtcatgg gcgaagtcga tcacctcgta cccgcgcgag gtctccggtg tcagctcgca
    30241 cagcggagtc gtgacgatgc gcggctcggt gaacccgtac tgcggcacac catccgtcgg
    30301 catctccgcg ccgactgcgg cgcggtagtc cggcccgagg tgagacttcg ctcgcgccat
    30361 gtctcacccc tccgagctac cccgacgcgg ccttcttccg aaaggcgaga accttgttgc
    30421 caggattcga cgcggccggc cccgcgggag cgggcttcga cgccgggcga cccggcccag
    30481 cggccggtgc ctcatcctca ggagtcagac cgagcgcgtc gaggtacttc aggaagctcg
    30541 cgattgacac gttgtcgttc gccggaacag cgggccgccc cttgccgagc gctgcgtcct
    30601 gcagcgccca gtcgacgatc tgatcccatg cgtcgatctt ccgagcaagg gcacgagccg
    30661 ccgagattgc cgccgagtga cgcgcccgca ggtgctttga gttcgcgatc gaccgctcga
    30721 gcgcctccga cacgctctgc cgctcgaact gggtcaccat gcacctcctc gcgcgcgcga
    30781 ccccctggtc acgaattacc ggggagagag gaacactccc ccgggcggtg tgccgtcccc
    30841 cacccgcttc ccggattttg gaagggggtg gggcgtggac ggcggcggac cgcggcactg
    30901 gggatgccgc cgggctcgac gctgccgccc acgctgatca ggctcggacg atctcgccgt
    30961 ccggtgatgc gtggatgatg gcgagcgcgt cctggtacag gtccatggag tcgaagccac
    31021 ggggcacgag gtcggcgtcg aggaatacga cgtcggcggt cagaccgcgc atgccctgac
    31081 caggacggcg gacgatgatg cgcccgctgt ctccatgtgc ccggatctcc tcgagaccat
    31141 tcgctcgacg cacggtcgcg ccgatcatgc ccggtgagcg ctgcaggtct tccatggccg
    31201 agtgcacgtc gctggtgatg acagcgacgt cgcgtcctcg gcgagcctcc gcgatcagcc
    31261 cggcagccgt gtacttgttc actgcgtccc ctcaccacca tgcgggcatc tgctcgccga
    31321 gaccgttctc gttcacgtcg cccttgccgc gcgagctgtt gcagctgaag tgcgcgtgcc
    31381 tgaagttgtc cgggtcgttc tgcagctccg ggtaatcgtc gaccgggtag tagtggtcga
    31441 gcgtgtgcga gtccggcgtc gtgccagggt cggcctcgta gtcgatgcgc gtgcggcaca
    31501 ggtggcagtc ggcaaggtgt cttgtctccg ggtccgcgtc gagacggcgc ccctcctcga
    31561 agaactccgt ccgcagacgc tgatcctcac gagtgtgggt gcgcttgctc acgacgacgc
    31621 tcctccgctc gacaccgccc acagaagatc acgtcgaaca cctgcgtcac gccgtgcgaa
    31681 ctcagccggt agccgtgcgt acgtcgcagc gcatcgaacc cgcacgctgg gcactcgccg
    31741 gacacctgct cgacgaggac gctcaccggg atcaccacgt gtgcgggcat ccggcgcctc
    31801 cgagagtgaa tgtgccctcc gctgcgactt cgccgtgatg ccatgtccgg aagcgcgggg
    31861 cgcagtccgg atgcgtggcc ggcgggatgc aggtcatgat ccaagcgacc gggcgctgcc
    31921 agtgggtaac ggcgacgccg agcgtgaccc cgaccgccag cagcaggacc ggcttcatga
    31981 cgacagcagg tgtctcatgt cgatcacgtg cgcgtcgagc tcgaccagcc ggtccatgaa
    32041 ccggccgcca tgatgggcgc agtacgcgag gcttgctggc cagtcctccc gggacacgta
    32101 cacgaacgcc tgcacatgcg cgtcatgcgg gcaggcgtcg cactcatccc ggtcgacctc
    32161 gaccaccacg gcgctcatga tgcgtccgcg tcggtgagat cgtcgatcgg ctccaccggg
    32221 tcacaatgcg tcaccgcggc gatcagagac gggcgcggcg aaccacaccc cgggcaaggc
    32281 caagacatga ccgcctccgc tccgagaccc agacacgacg aaagccccac ccgtaggcga
    32341 ggctttcagt cacttcttgt gcaagacaca gtctacacaa gatcggcggc cacgtttccc
    32401 tgatcagctc ggcgagtcgc cgccaggtgg tgcttcccaa cgcgggttcc atcccatggc
    32461 tcgaacccgg ggctccgcgc cgacagcgac gaacgccatc ggatgctccc ggcaccgatg
    32521 catcccgcca tggtcggcgt ctcccccaca gtggtggtca ccggtggggg acgtccacgc
    32581 gaaccgacac tgcgcagtca cgatgcacca ctctcggccg aatggtgcgg acacgcgcag
    32641 tccacgatct catccgtcgc ctcgtcgagc gcgcgcccgt cgcacgcgtc atgcttccca
    32701 tcccggcagt cgggccccaa ggtcccgacc gggcgggcgt agtcgaaggt gacgtactcg
    32761 ccctcccacg tccagtccac gtcaccatca gggaagtcct cgtcgcgcag cagcagctcg
    32821 tgctcgacgc ccgcgtacga gtcggccaga tcggtcgcgt cgatccacgg cctgatccag
    32881 cactcggcgt ggctggtgaa ctcatggccg cacggccagt actcgtgatc gcagtcgtcc
    32941 tccgggtact ggtggcacca tgcgctctcg tcgccggggc agatgaactt gccgccctcg
    33001 atgcggtgct gcaccgaggg gaacgccgac aggtcgggca tcttcggctg ctccggcatc
    33061 ggcgcgggca ctcggtcgcg gcctgccagc ttgtcgcacg cggggcaccc gagagtgtcg
    33121 gtcttcgtga tgtggtcgtg ctcagccatc gctcttgctc ccgtccgggc ggtctgtggc
    33181 ggctcggaag ccgcgctcgt aggcgtcggc gtagcaccgc cgcatgtgga tgtgatggcg
    33241 ctccgggtag acgcggaggg cgacggcctc agcctcgcgg cggatctcgt caggcatctc
    33301 gactcccgtc cgggcggttg ggtgtctcgt ctacggggag cccgaccccg tacgggttca
    33361 cgatcgtcgc aggtcgctca ccttcggggg cgctccacca tgcgaagacg gtcgcgatcg
    33421 cttcatccca tgtctcggct cgagccgcgc ggagatcctg cacggtcgcg ccctgttcgg
    33481 cttcccactc ggcgcgagat agcgcgcgtt cgaggatgaa gcgcatccag tgcgcgtccg
    33541 tccactcctc accgcatcgg cacttctcgg tggagtacag gtagcaatgc agtttgctca
    33601 tggaagcctt cgcatccagg gcttcggcca ccatgtcgtc cgtgacctgc tgtgcgatgt
    33661 tcatcggctc tctccgctcc gttccaccgg ccatgtcgcg ccggtctccg agttctcgtg
    33721 cttctcgtgc ggcccgacgc ggtgacaacg cattccagta cgggtcgacc tcctggtggc
    33781 ggaagatgtc ccacgaccag acctcggcct tgcacggctc gtcgtcggcc tcctgttcgg
    33841 cttcccactc ggcgcgcttc tgtgcatcgt gagcggccag ccagcgaaag aaggcggcat
    33901 ccgcctcttc catcgacatg aacgacccgt ccgggcgtcc acgtcgcaca gcggcggtac
    33961 agaagtcgat gcagatccga gcctcgctcg gcgtgtaatc gtcagccacg gtcggctcct
    34021 tcctctggct cgaacgtgaa catgtccgcc gggtagaaca tccgcgactc gatcggcccc
    34081 tcacccgccg tcgcgttcca tccgtcgcgg actccgtgct catccgtgcg gggacgctcc
    34141 acgacctcgc gcacggggat gcgcttgtcc ttccagacca tgtgcccctt gctcatcgct
    34201 tctctcctgt ctcattgaac gcggcacgca gagcggcctt ccacgcctga cgggtgatgc
    34261 ccatccgccg aggtgtcagg aagtcgtcat ccggaccgaa tgtgaactcg tggaacgcct
    34321 tctgtgctgc gtctgcctgc gcgtcggtcg gttcgccctg gaacccgccc aactccgtca
    34381 gccccgcatc cgtcaacccg gtcagggcgc ggacgtgatc gcggatagcc gccgcctcag
    34441 cacggatctc gtcgttcgac ttcggagacc cgttcgcgcg gcgaagcatc acctgcggct
    34501 cgtcctgcac ggcctcacat gcggcgcagc tcttgcgcat cgtgacgcgc cccggtgacc
    34561 agtgcgggca ctcgatgagc ttcggttcgc cctgcacgcc aacggacgga gcgatctcca
    34621 atgcggcttc gaccatcgcg tacgccttca ggtgctcgcc acgactgccg tagtgcagca
    34681 cataccgcag gacgtgagcg atgccgtgct tgcggtcatg ctcgggcgtg tggcccttct
    34741 cgatctgccg tgcacgctcg gcattgatct gctcctcggt gatcggctcc ggggatacgg
    34801 ggcgacggaa caggtactgc cgcacccatt cggcggtgtc ctcatgcgag ttc
//
LOCUS       DATMSX010000439         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101327,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000439 DATMSX010000000
VERSION     DATMSX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101327"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VN041_14115"
     CDS             complement(<1..618)
                     /locus_tag="VN041_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU30214.1"
                     /translation="MTEVSADLGTGRASVGAVLDFAWAAGEFTATDVMTATSLTRSTA
                     IDAVDTLVSARVLHELPNARAAGAYRAGRPARRFALSPDLGVVAGLDAGDTHLAVTIA
                     DLTESTLAHRRIDLSPAQSPAERRATILEHLELALADSGHSRDDLLAICAGVAAPVNR
                     DGASPPHPDGFWERTNPDLASGLAGWAPVVEVENDALLAAVAEGSR"
     gene            721..2052
                     /locus_tag="VN041_14120"
     CDS             721..2052
                     /locus_tag="VN041_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30215.1"
                     /translation="MSARPFSRLRLAAGALAIALVSTALAGCSADGRETIRFTFSKRE
                     AIDFMTEVVAKYNASQDKVRVQIDTSGVDVVSASFVRGNPPDVMLANYNFEVARFVQR
                     CALSDLSGTRAAASLRDDLQPLMDQYGSCAGRVSALPYSVMAASVIYNKQIFDEQGIQ
                     VPKTWDELIAACDQLKAAGITPFYGTFKDDWTVGQGWYDYSLGGSVDVVDFFDRLNAE
                     GADVGPDSSVSFQKDFARPISRMETLVKDYVNPDAASRGYGDGNLAFAQGKAAMYLQG
                     PWAFSEIAKTAPDLQLGTFPLPMTDDPSQLAVRVNMDLAAMIPDGSRHKAAARDFLEY
                     LYQPERIKAYNDSQLGFTPTKDAAAPDDPRVAGMTEYYDKGRIYQGPSVLVPKTIPVF
                     NYAQAIVLGEDPKSILKTMDADWARIAFRAPVVKADASASAASPETEESGE"
     gene            2049..2960
                     /locus_tag="VN041_14125"
     CDS             2049..2960
                     /locus_tag="VN041_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30216.1"
                     /translation="MSTTTSIVTAGDSTRRRSTRRVEPIYYLFLLPTLLLFTLAITVP
                     GVIGIFFSFTDSIGIGEWSFNGLTNYIALFSDPAILQSYLFTFGFSIATVIVVNVIAF
                     LLAVGLTSRIRFKTGLRAIFVIPMVISGIVIAYVFNFLFSNSIPAAGAATGIPWLATS
                     LLANPDLAWIAIVIVTAWQAIPGTLLIYIAGLLSVPGDVYEAADIDGASKRQQLLRIT
                     LPLVAGYVVINVILGFKGFLNAYDIIVGLTNGGPGTSTRSVAMTIIAGFNSGDYAYQM
                     ANATIFFIVAVLISLLQLSLTRGRNAL"
     gene            2960..3835
                     /locus_tag="VN041_14130"
     CDS             2960..3835
                     /locus_tag="VN041_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30217.1"
                     /translation="MSTQTITTQTAGKKPRVRMERVNWSGTIILILCAVTVLLPLYVT
                     ISMAFKTTGQAVDGNAFSLPAPFSIDGFVEAWNLTKFPVGAGISLLVTAGTVIATILL
                     AAFASYAIVRNWDRRLFRYSFFYLLAAMFIPFPVVALPQIQLTGRVGLDNPLGVIILA
                     TMFQLSFSVLLFTAFLRSIPIELEESARIDGATTWQTFWQLIFPLLAPMSATVGIFAF
                     LYAWNDFMMPSLIISDPAMQTLPVRQNLFQTQFSNNYNVSFASYLMAMAPAILAYLFT
                     QRFVMEGVTQGAVKG"
     gene            3882..>4248
                     /locus_tag="VN041_14135"
     CDS             3882..>4248
                     /locus_tag="VN041_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWU30218.1"
                     /translation="MTEALLTETREDEKTAAWWRQAAVYQIYPRSFADANGDGLGDIP
                     GIVSRADYLAELGIDAVWLSPFYPSALADGGYDVADYRNVDPRLGTLEDFDDMVAALH
                     ERGIRVVVDIVPNHTSDLHE"
BASE COUNT          712 a         1464 c         1335 g          737 t
ORIGIN      
        1 ccgcgagccc tcggcgaccg ccgccagcag agcgtcgttc tcgacctcga ccacgggagc
       61 ccaaccggcc aggcccgagg cgaggtcggg attcgtgcgc tcccagaagc cgtcgggatg
      121 cgggggagag gcgccgtcgc gattgacagg ggccgcgaca cccgcgcaga tcgccagcag
      181 gtcgtcgcgg gagtgccccg aatccgccag agcgagctcc aggtgctcga gaatcgtcgc
      241 acgccgctcg gcggggctct gcgcggggct gaggtcgatg cgtcggtggg cgagggtgga
      301 ctccgtgaga tcggcgatcg tcacggccag gtgcgtgtct ccggcgtcga gacctgcgac
      361 gacgccgagg tccggggaga gcgcgaagcg gcgggcgggc cggcccgccc gataggcgcc
      421 ggccgcccgg gcgttgggca gctcgtgcag gacgcgggcg ctgacgagcg tgtcaacggc
      481 atcgatcgcg gtcgaacggg tgagggacgt cgccgtcatc acgtccgtcg ctgtgaattc
      541 ccctgcggcc caggcgaagt cgaggacggc gccgacgctg gcgcgtcccg tgccgagatc
      601 cgcggagact tccgtcattt ctgctcttga cctttcgtgt cctcgatgag agagtaccgg
      661 tcagagtaat agattcgctc actagattta ataatcgaat gtacgaatgg aaggacgaag
      721 atgtctgcga gacccttctc gagactccgg ctcgccgccg gcgcgctcgc gatcgccctg
      781 gtgtcgaccg cgttggcggg ctgctcggcg gacgggcgcg agaccatccg gttcacgttc
      841 agcaagcgcg aggccatcga cttcatgacc gaggtcgtcg cgaagtacaa cgcctcccag
      901 gacaaggtgc gcgtccagat cgacacctcg ggcgtggacg tcgtctctgc gagcttcgtc
      961 cgcggcaacc cacccgacgt catgctcgcc aactacaact tcgaggtcgc gcgcttcgtg
     1021 cagcgctgcg cgctgagcga tctctccggc acgcgcgccg ccgcctcgct gcgcgacgac
     1081 ctgcagcccc tgatggacca atacggctcc tgcgccggtc gtgtcagcgc gctgccgtac
     1141 tcggtcatgg ccgcctcggt gatctacaac aagcagatct tcgatgagca gggcatccag
     1201 gtgccgaaga cctgggacga gctgatcgcg gcgtgcgacc agctcaaggc cgcgggcatc
     1261 actcccttct acggcacgtt caaggacgac tggacggtgg gacagggctg gtacgactac
     1321 tccctcggcg gctccgtgga tgtcgtggac ttcttcgatc ggctcaacgc cgagggcgcc
     1381 gacgtgggcc cggattcgtc ggtctcgttc cagaaggact tcgcccgccc catcagccgg
     1441 atggagacgc tcgtgaagga ctacgtcaac ccggatgccg ccagccgcgg ctacggcgac
     1501 gggaacctcg cgttcgccca gggcaaggcg gcgatgtacc tgcagggccc ctgggccttc
     1561 agcgagatcg cgaagaccgc ccccgacctg cagctgggca ccttccccct gcccatgacc
     1621 gacgaccctt cgcagctcgc cgtgcgggtg aacatggacc tcgccgcgat gatccccgac
     1681 ggctccaggc acaaggcggc cgcgcgcgac ttcctcgagt acctgtacca gcccgagcgg
     1741 atcaaggcct acaacgactc ccagctcggc ttcaccccca cgaaggacgc tgcggcgccc
     1801 gacgatccgc gcgtcgcggg catgaccgag tactacgaca agggccggat ctaccagggg
     1861 ccctccgtgc tggtgcccaa gaccatcccc gtcttcaact acgcgcaggc catcgtgctc
     1921 ggagaggacc cgaagtcgat cctgaagacg atggacgccg actgggcccg catcgcgttc
     1981 cgcgcgccgg tcgtgaaggc cgacgcctcg gcatccgccg catcacccga gacagaggag
     2041 tccggagaat gagcaccacg acgtcgatcg tcaccgccgg tgacagcacc cggcggcgca
     2101 gcacgcggcg cgtcgagccg atctactacc tgttcctgct ccccactctg ctgctgttca
     2161 ccctggccat caccgtgcct ggcgtgatcg gcatcttctt cagcttcacc gactccatcg
     2221 ggatcggcga gtggagcttc aacgggctga ccaactacat cgcgctgttc agcgatccgg
     2281 ccatcctgca gagctacctg ttcaccttcg ggttctccat cgcgacggtg atcgtcgtga
     2341 acgtgatcgc cttcctgctc gcggtcggcc tcacctcccg catccgcttc aagaccgggc
     2401 tgcgggcgat cttcgtgatc ccgatggtca tctcgggcat cgtcatcgcc tatgtcttca
     2461 acttcctgtt ctcgaactcg atccccgcgg cgggcgcggc gacgggcatc ccgtggctgg
     2521 cgacgagcct gctcgcgaac cccgacctcg cctggatcgc gatcgtcatc gtcaccgcct
     2581 ggcaggcgat cccgggaacg ctgctgatct acatcgcagg gctcctctcg gtgcccggtg
     2641 atgtgtacga agccgccgac atcgacggag cgagtaagcg gcagcagctg ctgaggatca
     2701 cgctgccgct ggtcgcgggc tatgtggtga tcaacgtgat cctcggattc aagggcttcc
     2761 tgaacgccta cgacatcatc gtcggcctca ccaacggcgg gccagggacc tcgacccgca
     2821 gcgttgcgat gacgatcatc gccggcttca acagcggtga ctacgcctac cagatggcga
     2881 atgcgacgat cttcttcatc gtcgccgtac tgatctcgct ccttcagctc tcgctgaccc
     2941 gcggaaggaa tgccctctga tgtcgacgca gacgatcacc acccagaccg cggggaagaa
     3001 gccgcgcgtg cgcatggagc gtgtgaactg gtccggcacg atcatcctga tcctgtgcgc
     3061 ggtcaccgtg ctcctgccgc tctacgtcac gatctcgatg gcgttcaaga ccacagggca
     3121 ggcggtcgac ggcaacgcat tctccctgcc ggccccgttc agcatcgacg gtttcgtcga
     3181 ggcgtggaat ctcaccaagt tcccggtcgg cgcagggatc tcactgctgg tcactgccgg
     3241 caccgtgatc gcgacgatcc tgctcgcggc cttcgcgtcg tacgcgatcg tgcgcaactg
     3301 ggatcggcgg ctgtttcgct attcgttctt ctatctgctc gcggccatgt tcatcccttt
     3361 cccggtggtg gcgctgccgc agatccagct caccggacgc gtcgggctcg acaacccgtt
     3421 gggcgtgatc atcctcgcga cgatgttcca gctcagcttc agcgtgctgc tgttcaccgc
     3481 gttcctgcgg tcgatcccga tcgagttgga ggagagcgca cgtattgacg gggccacgac
     3541 ctggcagacg ttctggcagt tgatcttccc gctgctcgcc ccgatgagcg cgaccgtggg
     3601 catcttcgcg ttcctctatg cgtggaacga cttcatgatg ccgtcgctca tcatctccga
     3661 tccggcgatg cagacgctgc cggtgaggca gaacctgttc cagacccagt tcagcaacaa
     3721 ctacaacgtg tcgttcgcct cgtacctgat ggcgatggcg cccgcgatcc tggcgtacct
     3781 gttcacgcag cgtttcgtca tggagggcgt aacgcagggt gcagtgaaag gctgacaccg
     3841 cagccaggac caccgcaaga aaagaaggag caccacccct catgactgaa gcgcttctca
     3901 ccgagacccg cgaagacgag aagacagcag cctggtggcg tcaggccgcc gtctaccaga
     3961 tctacccccg cagcttcgcc gacgcgaacg gggacggcct cggcgacata ccgggcatcg
     4021 tctcccgtgc cgactacctc gccgagctcg ggatcgacgc ggtgtggctg agcccgttct
     4081 acccgtccgc cctcgccgac ggggggtacg acgtcgccga ctaccggaac gtcgatcccc
     4141 gcctcggcac gctcgaggac ttcgacgaca tggtcgcggc actgcacgag cgcggcatcc
     4201 gcgtggtcgt cgacatcgtc ccgaatcaca cctcggacct gcacgagt
//
LOCUS       DATMSX010000440         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_177198,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000440 DATMSX010000000
VERSION     DATMSX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177198"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VN041_14140"
     CDS             <1..327
                     /locus_tag="VN041_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWU30219.1"
                     /translation="GVPAIDAALDAAGTAEATESSLALVGDRRRIVTIAAPRDAAEHG
                     FQASSGSDPETARFRDAARIRLVEAAERGDLVVPMAREYPLSQAPEALRFLAEGHPGG
                     KIALVP"
     gene            501..2255
                     /gene="leuA"
                     /locus_tag="VN041_14145"
     CDS             501..2255
                     /gene="leuA"
                     /locus_tag="VN041_14145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWU30220.1"
                     /translation="MKNNQKPSSMPVHKYRPFHEQIRVDLPDRTWPSKRITEAPRWCA
                     VDLRDGNQALIDPMSPERKRVMFELLVSMGYKEIEVGFPSASQTDFDFVRQLIEEDLI
                     PDDVTIQVLTQCREHLIERTYEAITGAKQAIVHFYNSTSVLQREVVFRSDREGVKQIA
                     LEGARLCRKLEKTIPDTEVYYEYSPESYTGTELEYAVEVCNEVIEVLEPTPDRKVIIN
                     LPATVEMASPNVYADSIEWMHRHLAQRENVILSLHPHNDRGTAIAAAELGYMAGADRI
                     EGCLFGNGERTGNVDLVALGVNLFTQGIDPQIDFSDIDQVKRTVEYCNQLPVPERSPW
                     AGDLVFTAFSGSHQDAIKKGFEAMAAKAEEQGVSVDDIEWGVPYLPVDPKDLGRSYEA
                     VIRVNSQSGKGGVAYLLKTDHALDLPRKLQIEFSGVVQAKTDAEGGEVTSDQIWSIFH
                     DEYLPADDDAAKWGRFELLGTQTRSDMTGEVSLDVVLRDGEERVATAGTGNGPIAAFI
                     EILRARGFDVSLYDYVEHTMSSGGDAQAAAYVELQVDDHRLWGVGIDGDISTASLKAI
                     VSCVNRSIRTREAQLVEA"
     gene            complement(2367..3053)
                     /locus_tag="VN041_14150"
     CDS             complement(2367..3053)
                     /locus_tag="VN041_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HWU30221.1"
                     /translation="MTEPLFTIPLWADLLGVGLGGIQGAMFASGFQGERRLDWLGVAI
                     IGIMIGMGGGLIRDLLLGQPPSTLQSNWYLITAAGAALFGMLLAGVFNRINTFIVVLD
                     AVVIGMFGAFGVSKAIAFGIPPVPAVFIGVCAAVGGGVLRDVLMGLPVAIMHVGSLYA
                     VAAGVGCTATVVMHLFDVDITAAAITGIIVTAVIRVLAVIYDVSLPEQRRLYRRKVAT
                     ETGMIPIIKP"
     gene            3090..>3563
                     /gene="recO"
                     /locus_tag="VN041_14155"
     CDS             3090..>3563
                     /gene="recO"
                     /locus_tag="VN041_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWU30222.1"
                     /translation="MPTYRDEAVVLRTHKLGEADRIVTMLSRRNGKIRAVAKGVRRTS
                     SKFGSRLEPFMVADVQLYQGRSLDIVQQAESLGAYGADIAVDYERFTAASAMVETADR
                     LNEAEATSEQYLLLVGGLRSLSRGEHAARSILDSYLLRVMALSGWAPALEDCARCG"
BASE COUNT          617 a         1204 c         1209 g          533 t
ORIGIN      
        1 ggcgtcccgg cgatcgacgc cgcgctggat gcggcgggca ccgccgaggc caccgagtcc
       61 tctctcgctc tcgtcggcga tcggcggagg atcgtcacca tcgcagctcc ccgggatgcg
      121 gccgagcacg gattccaggc gagctccggc tcggaccccg agacggcgcg gttccgcgat
      181 gcggcacgca tccggctggt cgaggccgcg gaacgcggcg atctggtcgt gccgatggcg
      241 cgggagtacc cgctctcgca ggcgcccgag gcgctgcgct tcctggccga gggacacccc
      301 ggcgggaaga tcgccctcgt cccctgaccc tgctggcgtc acgtggtgag cattctgcca
      361 agcggggtgt tagcatcgtt gcatgcctct gggcggtctg cttcttctta gctgccgcga
      421 cgagtcctga tcatcgggcc tctctcgtcg cggcgcttgt gttggcctga gcacgttttc
      481 tgacaagaag aagccccatc atgaagaaca accagaagcc ctcctcgatg ccggtccaca
      541 agtaccggcc gttccacgag cagatccgcg tcgatctccc ggatcgcacc tggccctcga
      601 agcgcatcac cgaagcgccc cgctggtgcg cggtcgacct gcgcgacggc aaccaggcgc
      661 tcatcgaccc gatgtcgccc gagcgcaagc gcgtgatgtt cgagctgctc gtgagcatgg
      721 gctacaagga gatcgaggtc ggcttcccat cggccagcca gaccgacttc gacttcgtgc
      781 gtcagctgat cgaggaggac ctgatccccg atgacgtcac gatccaggtg ctgacgcagt
      841 gccgtgagca cctcatcgag cgcacctacg aggcgatcac gggcgccaag caggccatcg
      901 tgcacttcta caactcgacc agcgtgctgc agcgcgaggt cgtgttccgc tccgaccgcg
      961 aaggcgtcaa gcagatcgca ctggagggtg cgcgcctgtg ccgcaagctg gagaagacca
     1021 tccccgacac cgaggtgtac tacgagtact ccccggagag ctacacgggc accgagctgg
     1081 agtacgccgt cgaggtgtgc aacgaggtga tcgaggtgct ggagcccacg cccgatcgca
     1141 aggtgatcat caacctgccc gccacggtcg agatggccag ccccaacgtg tacgcggact
     1201 cgatcgagtg gatgcaccgg cacctggcgc agcgcgagaa cgtgatcctg tcgctgcacc
     1261 cgcacaacga ccgcggcacc gcgatcgccg cagcagagct cggctacatg gcgggagccg
     1321 accgcatcga gggatgcctg ttcggcaacg gggagcgcac cgggaacgtc gacctggtcg
     1381 ccctgggcgt caatctgttc acgcagggga tcgacccgca gatcgacttc agcgacatcg
     1441 accaggtcaa gcgcacggtc gagtactgca accagctgcc cgtgcccgag cgcagcccgt
     1501 gggcaggaga cctcgtcttc accgcgttca gcggttcgca ccaggacgcc atcaagaagg
     1561 gcttcgaggc catggccgcg aaggccgagg agcagggcgt ctcggtggac gacatcgagt
     1621 ggggcgtgcc gtacctgccg gtcgacccca aggacctggg ccgctcgtac gaagctgtca
     1681 tccgggtgaa ctcgcagtcc ggcaagggcg gcgtcgccta cctgctgaag accgaccacg
     1741 cgctcgacct gccgcgcaag ctgcagatcg agttctcggg cgtcgtgcag gcgaagacgg
     1801 atgccgaggg cggcgaggtc accagcgacc agatctggtc gatcttccac gacgagtacc
     1861 tcccggcgga cgacgacgcc gccaagtggg ggcgcttcga gctgctcggc acgcagaccc
     1921 gcagcgacat gaccggtgag gtgtccctcg acgtggtact gcgcgacggc gaggagcggg
     1981 tcgcgaccgc cggcacgggg aacggcccga tcgcggcctt catcgagatc cttcgcgcac
     2041 gcggcttcga cgtgtcgctg tacgactacg tggaacacac catgtccagc ggtggcgacg
     2101 cccaggccgc ggcctacgtc gagctgcagg tggacgacca ccggctgtgg ggcgtcggca
     2161 tcgacggcga catctcgacc gcatccctca aggcgatcgt gtcctgcgtg aaccgctcga
     2221 tccgcacccg tgaggcccag ctcgtcgagg cctagtccta cttcgccgag accccccgtt
     2281 cgcgtcgaga cccccaactg cctgcgccgg tagctggggg tctcgacggt aattaggggt
     2341 ctcggcgtca gggtgggggt tcctggtcac ggcttgatga tcgggatcat ccccgtctcg
     2401 gtcgccacct tgcggcggta cagacggcgc tgctccggca gcgacacgtc gtagatgacg
     2461 gcgagcacgc ggatgacggc ggtcacgatg atgccggtga tcgcggcggc ggtgatgtcg
     2521 acatcgaaca ggtgcatgac gaccgtggcc gtgcatccca ccccggcggc gaccgcgtag
     2581 agcgaaccga cgtgcatgat cgcgaccggc agccccatca gcacgtcgcg caggacgccg
     2641 ccgccgacgg ccgcgcacac gccgatgaac acggcgggga ccggagggat gccgaaggcg
     2701 atggccttgc tgactccgaa cgctccgaac atcccgatca ccacggcgtc gagcacgacg
     2761 atgaaggtgt tgatgcggtt gaagacaccc gccagcagca ttccgaacag cgcggcgccc
     2821 gcagcggtga tgaggtacca gttgctctgc agcgtcgaag gaggctgacc gagcagcagg
     2881 tcgcggatca gaccgccgcc catgccgatc atgatgccga tgatcgcgac gcccagccag
     2941 tcgagacgcc gctcgccctg gaagccggac gcgaacatgg cgccctggat gccgccgagc
     3001 ccgacgccga gcagatcggc ccacagaggg atggtgaaga gcggttcggt cacccgtcca
     3061 ttcttccctg tgcaggggat aatcgttgag tgcccaccta ccgagacgaa gcggtggtcc
     3121 tgcgcaccca caagctgggt gaggcggacc gcatcgtcac gatgctctcc cgccgcaacg
     3181 gcaagatccg cgcggtcgcg aagggtgtgc ggcgcacctc ctcgaagttc ggctcgcggc
     3241 tcgagccgtt catggtcgcc gacgtgcagc tgtaccaggg ccgctcgctg gacatcgtgc
     3301 agcaggccga gtcgctcggt gcgtacggcg ccgacatcgc ggtcgactac gagcgcttca
     3361 cggccgccag cgccatggtc gagacggccg accggctgaa cgaggcggag gcgacgagcg
     3421 agcagtacct gctgctggtc ggcggcctga gatcgttgtc gcgcggcgag catgccgccc
     3481 gcagcatcct ggactcctac ctgctgcgcg tgatggcgct gtccggctgg gcgccggcgc
     3541 tggaggactg cgcgcgctgc ggc
//
LOCUS       DATMSX010000441         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_186698,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000441 DATMSX010000000
VERSION     DATMSX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186698"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="VN041_14160"
     CDS             complement(<1..2165)
                     /locus_tag="VN041_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HWU30223.1"
                     /translation="MRVITAENRVVWSASDLKLAAECEFAWARAVDAKLGRVAAVEEP
                     EDATLERAARLGDRHEEQVLGRYIARLGESVDGGPGVVTLPKVSSTDAEAMDAVISDT
                     VRALRSEASVVYQAAFSTPEFVGFADFITRDEVDGRWRVQDSKLARTARATALMQLAA
                     YVDQLDRLGIPRSDEVDLILGDGTTSTHRVQDVLPLFHVRRARLRAIIADRAIETGAA
                     GAPLAWGDDRGDLQLTACGRCATCEEQVIAHRDLLMVARMRPVQRQRLRAAGILTVDD
                     LATASAAPDGMNSDTFTALRAQARLQAGLTLEPGEAPPFELVSPNAIGMMPRPSRGDL
                     FFDFEGDPLYTEPVASPADKPHWGIDYLFGWTDNDEQYSALWAHTFADEKRALLDFLE
                     FVKLRRQTNPDLHIYHYAPYETSHLAAMAARHGVGEADVDRLLREGVFVDLYPIVLRS
                     VRIGSRSYSIKKLEPLYMGAEVRTSDVQKGDDSIVQYVQARALAAAGQDAEAQAILDD
                     LADYNRYDCVSTRRLRDWLIGLARQEGVLPAPPDDPETRAYEPAPLSLALQAEAMNAE
                     REGRSGAEFRVAAAAIDYHPREAKSFWQGHFQRLREPVSIWESTRDVLRIDASSSGVD
                     EDWGVAEGQRVQTRLLTLRGEVAPGSTIGVGGRPFALYAVPAPFSAQAPSRVIHVPHE
                     VEVVEVLDDGYVVRERTVGGQTWDELPLALAPAAPPNAAT"
     gene            complement(2275..3093)
                     /gene="nadE"
                     /locus_tag="VN041_14165"
     CDS             complement(2275..3093)
                     /gene="nadE"
                     /locus_tag="VN041_14165"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HWU30224.1"
                     /translation="MTLQQQIAEDLGVKPEIDPAAEAETRIAFLVDYLHAARAKGFVL
                     GISGGQDSTLAGRLAQLAVERVRAGGGDATFVAVRLPYRVQADAADAEAAIDFIAADR
                     SLTINIEHGVDGLERDLEEGVGESISDFNRGNIKARLRMVTQYALGGHDGLLVIGTDH
                     AAEAVTGFYTKFGDGAADILPLSGLSKRQGRAILQHLGAPERLYLKVPTADLLDGVPG
                     RPDEDELGLTYEQIDDYLEGRDVPAEVSSLIEAKYLATRHKRHLPVTPGDDWWR"
     gene            3143..3709
                     /locus_tag="VN041_14170"
     CDS             3143..3709
                     /locus_tag="VN041_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HWU30225.1"
                     /translation="MKLLVAALASELQAFPAELRGFDRLVTGPGKLMAAVGLTRALDA
                     HLAEQYEEIVVVGTAGAVSDDVESGIYDVAAAIQHDVQDLDGVIGQHVSLPTRVETGR
                     VGVTIATGDIFVDDADATARIRSLGGVLVDMETFALVWVAQQFGVPIRVLRVVSDRAQ
                     DGATQVWDEVVAACSAQLWAHVQREYEV"
     gene            complement(3720..>4371)
                     /locus_tag="VN041_14175"
     CDS             complement(3720..>4371)
                     /locus_tag="VN041_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HWU30226.1"
                     /translation="CTCAHSCALQAALTATVDAAASEHAATAADARDFEILRSLADTL
                     AGRSGNTRRMNLETFVLAAELEEIVAAANLRLRDMSDGRYELRHSDAVAARGAASGLG
                     IVVFDAFTGQTRPAKSLSGGETFLSSLALALGLAEVVTARAGGIRLDTLFIDEGFGSL
                     DSDTLETAMRTLDELRQGGRTVGVISHVEAMQEQIPAQLTVRALPDGPSVIDAPRA"
BASE COUNT          613 a         1509 c         1571 g          678 t
ORIGIN      
        1 gtcgccgcgt tcggcggagc ggcaggcgcg agcgcgagcg gcagctcgtc ccaggtctgt
       61 cccccgacgg tgcgctcgcg cacgacgtag ccgtcgtcga gcacctcgac gacctcgacc
      121 tcgtgcggca catggatcac gcgcgacggc gcctgcgccg agaacggcgc aggcacggcg
      181 tacagcgcga acggacgccc tcccacgccg atcgtgctgc cgggtgcgac ctcgccgcgc
      241 agtgtcagca ggcgcgtctg cacgcgctgc ccctcggcga cgccccagtc ctcgtcgacg
      301 ccgctggacg atgcgtcgat gcgcagcacg tcgcgggtgg actcccagat cgagacgggt
      361 tcgcgcagac gctggaagtg accctgccag aagctcttgg cctcacgggg gtggtagtcg
      421 atcgccgctg cggcgacacg gaactccgcc ccgctgcgcc cctccctctc ggcgttcatc
      481 gcctcggcct gcagagccag cgacagcggc gcgggctcgt aggcgcgggt ctccggatcg
      541 tccggcggcg cgggcagcac gccctcctgg cgtgcgaggc cgatcagcca gtcccgcagc
      601 ctcctggtcg acacgcagtc gtaccggttg tagtcggcga ggtcgtcgag gatggcctgc
      661 gcctcggcat cctggcccgc cgccgccagc gcccgtgcct gcacgtactg gacgatcgag
      721 tcgtcgccct tctgcacgtc gctggtgcgc acctcggcgc ccatgtacag cggctcgagc
      781 ttcttgatcg agtaggagcg cgagccgatg cgcaccgagc ggagcacgat cgggtacaga
      841 tcgacgaaca cgccctcgcg cagcagccgg tcgacgtccg cctcgcccac gccgtgccgc
      901 gccgccatgg ccgcgagatg cgaggtctcg tagggcgcgt agtggtagat gtgcagatcg
      961 gggttggtct ggcgacgcag cttcacgaat tccaggaagt cgagcagagc gcgcttctca
     1021 tctgcgaagg tgtgcgccca cagggcggag tactgctcgt cgttgtcggt ccagccgaac
     1081 aggtagtcga tgccccagtg cggcttgtcg gccggcgagg cgaccggctc ggtgtacagc
     1141 gggtcgcctt cgaagtcgaa gaacaggtcg ccgcggctcg ggcgcggcat catcccgatc
     1201 gcgttcggtg agaccagctc gaacggcggg gcctcgccgg gttcgagggt gagtccggcc
     1261 tgcagtcgtg cctgcgcccg cagggcggtg aacgtgtcgc tgttcatccc gtcgggcgca
     1321 gccgatgccg tggcgaggtc gtcgacggtg aggatgcccg ccgcgcgaag gcgctggcgc
     1381 tgcacggggc gcatgcgggc gaccatgagc aggtcgcggt gcgcgatcac ctgctcctcg
     1441 caggtcgcgc agcgcccgca ggcggtgagc tgcaggtcac ctcggtcgtc tccccaggca
     1501 agcggagcgc cggcggctcc tgtctcgatc gcgcggtccg cgatgatcgc cctcagccgc
     1561 gcgcggcgca cgtggaacag gggcagcacg tcctggacgc ggtgggtgct ggtggtcccg
     1621 tcaccgagga tcagatcgac ctcatcggag cgcgggatgc cgagacgatc gagctggtcg
     1681 acatatgccg ccagctgcat gagcgcggtg gcacgcgctg tgcgagcgag cttggagtcc
     1741 tgcacgcgcc agcgcccgtc gacctcgtcg cgtgtgatga agtcggcgaa gccgacgaac
     1801 tcgggcgtcg agaacgcggc ctggtagacg acggatgcct cggagcgcag cgcgcgcacg
     1861 gtgtccgaga tgacggcatc catcgcctcg gcatccgtcg atgagacctt cggcagggtg
     1921 accactccgg gaccgccgtc gaccgactcg ccgagtcggg cgatgtacct gccgagcacc
     1981 tgctcctcgt gacggtcgcc gagcctcgcg gcgcgctcga gagtggcgtc ctcgggctcc
     2041 tccaccgcgg caacgcggcc gagcttggcg tcgaccgcgc gcgcccaggc gaactcgcat
     2101 tccgccgcga gcttcaggtc gctcgcactc cagacgacgc gattctccgc cgtgatgacc
     2161 cgcacgatgt ccctctccgt tgcccctctt cgaggttatc ggcagggtcg gacatcgccc
     2221 gacggccggc caggcctctc gagtccgccg gatcaggtgg tcacgcggtc gacgtcagcg
     2281 ccaccagtca tcgcccggtg tcaccggcag gtgccgcttg tgccgggtgg cgaggtactt
     2341 cgcctcgatg agcgatgaca cctcggcggg cacgtctcgc ccctcgaggt agtcgtcgat
     2401 ctgctcatag gtgaggccga gctcgtcctc gtctggacgt ccgggaacgc cgtcgagaag
     2461 atcggcggtc ggcaccttca ggtacagccg ctctggcgcc ccgaggtgct gcaggatcgc
     2521 acgcccctgc cgcttgctca gacccgagag cggcaggatg tcggccgccc cgtcgccgaa
     2581 cttcgtgtag aacccggtca ccgcctcggc cgcgtgatcg gtgccgatca ccagcagccc
     2641 gtcatgaccc ccgagcgcgt actgcgtgac catgcgcagg cgcgccttga tgttgccgcg
     2701 gttgaagtcg ctgatgctct cgccgacgcc ctcctcgagg tcgcgctcca gcccgtccac
     2761 gccatgctcg atgttgatcg tcagcgagcg gtcggccgcg atgaagtcga tcgcggcctc
     2821 ggcatcggcg gcatccgcct gcacgcggta cgggagccgc acggcgacga acgtcgcgtc
     2881 gcctccgccg gcccgcaccc gttcgacggc gagctgcgcg agccgtccgg cgagggtgga
     2941 gtcctggcca ccggagatgc cgagcacgaa gcccttcgct ctggcggcat ggaggtagtc
     3001 cacgaggaat gcgatcctcg tctccgcctc ggcggcgggg tcgatctcgg gcttcacgcc
     3061 caggtcttcg gcgatctgct gctgcagggt cacggggcct ccttcgtcgg gttccagtct
     3121 cccccactgc gagaatggat gcgtgaaact tctcgtcgcc gccctcgcct ccgaactgca
     3181 ggccttccct gccgagctcc gtggcttcga ccggctcgtg accgggccgg gcaagctgat
     3241 ggccgccgtc ggactgacca gggcgctgga tgcgcacctc gcggagcagt acgaggagat
     3301 cgtcgtggtc ggcacggcgg gcgcggtgag cgatgacgtc gagtccggca tctacgacgt
     3361 cgcggccgcc atccagcacg acgtgcagga tctggacggc gtgatcggcc agcacgtctc
     3421 cttgccgacg agggtggaga cgggtcgcgt gggtgtgacc atcgcgaccg gcgacatctt
     3481 cgtcgacgac gcggatgcca cagcgcgcat ccgctcgctc ggcggcgtgc tcgtcgacat
     3541 ggagaccttc gccctcgtgt gggtggcgca gcagttcggc gtgccgatcc gcgtgctgcg
     3601 ggtggtctcg gaccgcgcgc aggacggcgc gacgcaggtg tgggacgagg tcgtggcggc
     3661 ctgcagcgcg cagctgtggg cgcacgtgca gcgcgaatac gaggtctgag gccggagctt
     3721 caggcccgag gagcgtcgat cacgctcggg ccgtcgggca gcgcccggac ggtcagctgc
     3781 gcggggatct gctcctgcat ggcctcgacg tgactgatca caccgaccgt gcgcccgccc
     3841 tgacgcagtt cgtcgagggt gcgcatcgcg gtctcgagag tgtcggagtc gagtgacccg
     3901 aagccctcat cgatgaacag cgtgtccagg cggatgccgc cggcgcgagc cgtgacgact
     3961 tcggcgagtc ccagcgcgag ggcgagcgag gacaggaacg tctcgccgcc ggagagcgac
     4021 ttggccggcc ttgtctgccc ggtgaaggcg tcgaagacga cgatgcccag accggatgcc
     4081 gcgcctcggg cggccacggc gtcggagtgc cgcagctcgt agcgtccgtc ggacatgtcc
     4141 cgcagccgca ggttggccgc ggcgacgatc tcctcgagct cggccgcgag cacgaaggtc
     4201 tcgagattca tcctgcgggt gttgccggag cggccggcga gcgtgtcggc gaggctgcgg
     4261 agaatctcga agtcgcgcgc gtccgccgcg gtggctgcgt gctcggatgc cgcggcatcg
     4321 accgtcgcgg tgagggcggc ctgcagcgcg cagctgtggg cgcacgtgca g
//
LOCUS       DATMSX010000442         7069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101375,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000442 DATMSX010000000
VERSION     DATMSX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101375"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VN041_14180"
     CDS             <1..387
                     /locus_tag="VN041_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30227.1"
                     /translation="QRAARLGADRLAHAPFTERLTDEEIAEQAASVAWISTLAIHDDA
                     HRAIAIDNVRRFADAGGTVLYGTDMGNGPTPVDLNRRELDALREAGIDGIRLLRALAP
                     RDHDPHPLFLPDGDPAAARPLTHLEA"
     gene            389..1669
                     /locus_tag="VN041_14185"
     CDS             389..1669
                     /locus_tag="VN041_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU30228.1"
                     /translation="MTESSTPIESAEDEHRRVAGISAPDLLSEARRLDAADPLAHHLD
                     AFADAPGVTAYLDGNSLGRPLRDLPEKVGAFIREDWGTRLIRSWDEQWMALPMQLGDR
                     IAEVALGAAAGQTVVADSTSVLLYKLMRAAVAARPGRDEIVIEAGNFPTDRFIAAGIA
                     KETGATLRWVEPDEVAGVTASDVAAVVSERTALVVLSHVDYRSGALADMPAITSVVHD
                     AGALMLWDLCHSVGVVPMQLDEWGVDLAVGCTYKYLNGGPGSPAFAYLRAEHHGAVIQ
                     PIHGWWSASDIFAMGPEYAPVGGIRQLLSGTPPVMSMLAMQGMLDLIASAGMPAVRAK
                     SISLTDFAVRAYDELLAPLGVRLLTTRDAAHRGGHITIGHDSFQQVTQQLWAEGIIPD
                     FRFPDGIRLGLSPLSTSHEETLRGILAVRDALTA"
     gene            1674..2474
                     /locus_tag="VN041_14190"
     CDS             1674..2474
                     /locus_tag="VN041_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWU30229.1"
                     /translation="MTSSVLDDIDARPGSTTSLLRTLIGLYLRPLGGTISSGALVGLA
                     EDLGIPAARARTAITRLKKHGLLLAASGGYALNPAAVPMLERGDRRIFAVRTMRAGDE
                     WMLVSATIPETRRDLRHQLRRRLQFLGAGAVSAGLWILPGHLDAEVEELLDELGARGF
                     ATLFRTSDPRPAQPLVQAVTAWWDLEALRAEHEQFLASVTGLDLSTPFAAYVRLIDSW
                     RVLPYIDPGLPPELLPADWPGVRSMETFAELSALLAEPAGEHVRSVAS"
     gene            complement(2580..3221)
                     /locus_tag="VN041_14195"
     CDS             complement(2580..3221)
                     /locus_tag="VN041_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWU30230.1"
                     /translation="MTTLGIIGAGHIGSNVARAAIAAGHDVVIANSRGPETLAELVAE
                     LGPKARAATAAEAGAAGDVVVVTVPLGAIEDIPVEPLAGKIVLDTNNYYFERDGHIDA
                     LDRGETTTSEIVQRHLPTSKVAKAFNHIVAAQITTDGSPAGTPNRRALATAGDDPAAV
                     EFVTRLYDELGFDTVNIGPLSESWRVERDRPAYVVRQNAAELEANLAIANRLP"
     gene            complement(3378..3860)
                     /locus_tag="VN041_14200"
     CDS             complement(3378..3860)
                     /locus_tag="VN041_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FBP domain-containing protein"
                     /protein_id="HWU30231.1"
                     /translation="MRPIDERAIRASFRNASRKEVSSLNLPLDFDEIDFEKIDFLGWF
                     DPKMPRRAYVVAEIDDHNVGILLQRTEQRTAMRAQCSWCDDVTLRNDVQFFSARKAGA
                     AGRNGDTIGTLICANFGCSANVRKLPPLAYEGYDRELARELRMLRLREHVTSFVRELG
                     "
     gene            4177..5811
                     /locus_tag="VN041_14205"
     CDS             4177..5811
                     /locus_tag="VN041_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWU30232.1"
                     /translation="MATKPGAGMPLRDYRIHRYVNAFCPRCHEEKPDQPLSEVRRLSG
                     WLADRDGTVWLERGCPEHGLVSTLYDENADILRYLEQWTAPTKVHTPDTMGNFKPVPQ
                     AYEDGLPQMQTQHTCILLEDITDHCNLKCPTCFAESSPAASAVAPLAEVLASVDAKLS
                     RENDRIDVLMLSGGEPTLYPWLEQLIEHLVARPIVRILLNSNGLRIANDDEFVQFLKK
                     HRERVEVYLQYDGEEAASVAFHRGADIRRFKERALERLSDAGIFTTLTMTAALGVNDH
                     EIGNVIRRAQATPYVGGVTIQPVFGSGRNSGIDPNERLTHTGVLARIGPQTKGEITWR
                     DLTALPCSHPHCCSVGYFLKDDSGDWKSLVGLIGHERLKEFLDLNPDLIANRIADSNV
                     NKAIRDVVKNSLLDLLSEQSSLSHPSMGDIWRDICVNCDLGVGTLATLAASKLPGQHK
                     RMRAMLAERVLRITVKPFMDINTMIEERLTQCCVHVATVNEQTNAHQCAPFCALQAWA
                     PLSRTRISTALGRPASSMTEVRETGGRTRLPVVQAS"
     gene            5808..6140
                     /locus_tag="VN041_14210"
     CDS             5808..6140
                     /locus_tag="VN041_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30233.1"
                     /translation="MTWAPEPVEGHPLNQFDPAVGHPAPPFDPLRLCVFTTIAAISCV
                     LGPAAVLVFALVAIAGYAKARRGGLLRSRCKLGDTRLVLAYLSVLAVASAVGIPFWVM
                     LWMRMLGG"
     gene            6142..6954
                     /locus_tag="VN041_14215"
     CDS             6142..6954
                     /locus_tag="VN041_14215"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWU30234.1"
                     /translation="MFPTLHDIAPWLPRLDTHSVFVALGMLAAIAVFLIEKRRRGVRD
                     PRIVYLVLGAMAGAAIMARLGTWAQHLDPSKNLNLGEQLLRGNASVLSALVGAWLGVH
                     IAKKLCRYPGRTGDLFAPAVALAMAIGRIGCLLTEQPGTPTGAAWGIVLDADAAARTG
                     SPAGVPLHPSFVYEIVFHAVAFAVLWFWLRHRPIAAGETLTIYIAGYAVFRFLIEFVR
                     GNDVAWMGLTRPQLFLAVTIPLLLARIIWMLRRGRFRAEPGVERKVPDGQPA"
BASE COUNT         1025 a         2500 c         2445 g         1099 t
ORIGIN      
        1 cagcgcgcag cgcgcctggg ggccgaccgg ctcgcacatg cgcccttcac cgagcgcctc
       61 accgatgaag agatcgccga gcaggcggcg tccgtggcgt ggatctcgac gctcgcgatc
      121 cacgacgacg cccaccgcgc gatcgcgatc gacaacgtgc gccgcttcgc cgacgccggc
      181 ggcacggtgc tctacggcac cgacatgggc aacggcccca cccccgtcga cctgaaccgg
      241 cgcgaactcg atgccctgcg cgaggccgga atcgacggca tccgcctgct gcgcgcgctc
      301 gcgccgcggg accacgaccc ccaccccctc ttcctgcccg acggggatcc cgccgcggcc
      361 cgcccgctca cccacctgga ggcatgacat gaccgaatcg tcgaccccca tagaatccgc
      421 ggaggacgag caccgccgcg tcgccggcat cagcgcgccc gacctgctca gcgaagcccg
      481 tcggctcgac gccgccgatc cgctcgcgca ccacctggac gccttcgccg atgcgccggg
      541 cgtgaccgcg tacctggacg gcaactccct cgggcggccg ctgcgcgacc tgcccgagaa
      601 ggtcggcgcg ttcatccgcg aggactgggg cacgcgcctg atccgctcct gggacgagca
      661 gtggatggca ttgcccatgc agctcggcga ccgcatcgcg gaggtcgccc tcggcgccgc
      721 cgccggccag acggtggtcg cggactccac cagcgtgctg ttgtacaagc tcatgcgcgc
      781 ggccgtcgcc gcccgcccgg gtcgcgacga gatcgtgatc gaggccggca acttccccac
      841 cgaccggttc atcgcggcgg gcatcgcgaa ggagaccggc gcgacactgc gctgggtgga
      901 accggacgag gtcgccggcg tcacggcatc cgatgtcgcc gctgtcgtct cggagcgcac
      961 cgcgctggtc gtgctcagcc acgtcgacta ccgctcgggc gccctcgccg acatgcccgc
     1021 gatcacgtcc gtcgtgcacg acgcgggcgc gctgatgctg tgggacctgt gccactcggt
     1081 cggcgtcgtg ccgatgcagc tggacgagtg gggcgtggac ctcgccgtcg gatgcacgta
     1141 caagtacctc aacggcggtc ccggatcccc ggcgttcgcg tacctgcgcg ccgagcacca
     1201 cggcgccgtc attcagccca tccacggctg gtggagcgcg tccgacatct tcgcgatggg
     1261 cccggaatac gcgccggtcg gcggcatccg ccagctgctc agcggtactc cgccggtgat
     1321 gtcgatgctc gcgatgcagg gcatgctcga cctgatcgcg tctgccggga tgcccgcagt
     1381 gcgcgccaag tcgatctcgc tgacggactt cgcggtacgc gcctacgatg aactgctcgc
     1441 gccactgggc gtgaggctgc tcaccacgcg ggatgccgcg caccgcggcg gccacatcac
     1501 gatcgggcac gactcgttcc agcaggtgac gcagcagctg tgggccgagg ggatcatccc
     1561 cgacttccgg ttccccgacg gcatccgcct gggtctgtct ccgctgagca cctcgcacga
     1621 ggagaccctg cgcggcatcc tggccgtgcg cgacgccctg accgcctgag cccatgacct
     1681 cttcggtgct cgacgacatc gacgcacgcc cgggcagcac cacctcgctg ctgcggacgc
     1741 tgatcgggct gtatctgcgg ccgctcggcg ggacgatctc gtcgggtgct ctggtggggc
     1801 tggccgagga tctcggcatc cccgcagcgc gggcgcgcac ggcgatcacc cggctgaaga
     1861 agcacgggct gctgctcgcg gcatccggcg ggtacgcgct gaaccctgcg gcggtgccga
     1921 tgctggagcg cggcgaccgc cgcatcttcg ccgtacgcac gatgcgcgcc ggcgacgaat
     1981 ggatgctggt ctcggcgacc atccccgaga cgaggcgcga cctgcggcac cagctgcggc
     2041 gtcggctgca gttcctgggt gctggcgccg tctcggcagg gctctggatc ctgcccggcc
     2101 acctggatgc cgaggtcgag gagctgctcg acgagctggg cgcccggggg ttcgcgacgc
     2161 tgttccgcac ctccgatcct cggccggcgc agccgctcgt ccaggccgtc acggcctggt
     2221 gggatctgga ggcgctgcgc gccgagcacg agcagttcct ggcatccgtc accggcctcg
     2281 acctgtccac gccgttcgcc gcctacgtgc gcctgatcga cagctggcgc gtgctgccct
     2341 acatcgatcc gggcctgccg cccgagctgc tccccgcgga ttggcccggt gtccgcagca
     2401 tggagacgtt tgccgagctc tccgctctcc tggccgagcc ggccggagag cacgtccgct
     2461 ccgtcgcgtc ctgaaccgca tccggaccgg gcccgtcacc ggatcggaga cgcatcgccg
     2521 agagccctct cagacgcgtc tccgatccgg tccgggcggc tgcacgggtg agaaacaggt
     2581 cagggcaggc ggttcgcgat cgcgaggttc gcctcgagct ccgcggcgtt ctgacgcacc
     2641 acgtatgccg ggcggtcgcg ctcgacgcgc caggactcgc tgagcggtcc gatgttgacc
     2701 gtgtcgaacc cgagctcgtc gtagagacgc gtgacgaact cgaccgcagc ggggtcatcg
     2761 cccgcggtcg ccagtgctcg ccggttcgga gtgccggccg gcgaaccgtc ggtcgtgatc
     2821 tgcgccgcca cgatgtggtt gaaggccttg gcgaccttcg acgtcggcag gtggcgctga
     2881 acgatctcgg acgtggtcgt ctcaccccga tcgagcgcgt cgatgtgccc gtcgcgctcg
     2941 aagtagtagt tgttcgtgtc gaggacgatc ttgccggcga gcggctcgac cgggatgtcc
     3001 tcgatcgcac cgagcgggac ggtcaccacc accacgtcgc ctgcggcgcc cgcctcggct
     3061 gccgtggccg cgcgggcctt cggccccagc tccgccacga gctccgccag cgtctccggc
     3121 cctcgcgagt tcgcgatgac gacgtcgtgt ccggccgcga tcgccgcgcg cgcgacgttg
     3181 ctgccgatgt gtcctgctcc gatgattccc aatgtggtca tgtcagcggt caacgccgca
     3241 tcgggagtgt tattccgcgg tcgtaacgag gccggaacgg gtcgaagttg cagtccgcct
     3301 tcggcgtgga tcagaaacgc accgcagacc ggtgtccggc gtgcgtttct gatccacgcc
     3361 gcgagagcgt gacggcgtca gccgagttcg cgcacgaaag acgtgacatg ctcccggagg
     3421 cgcagcatcc gcagctcacg cgcgagctcg cggtcgtaac cctcgtaggc cagcggcggc
     3481 agcttgcgca cgttcgcgga gcagccgaag ttcgcgcaga tcagcgtccc gatcgtgtcg
     3541 ccgttgcggc cggcggcacc cgccttgcgg gcggagaaga actgcacgtc gttgcggagc
     3601 gtgacgtcat cgcaccacga gcactgcgcg cgcatggccg tgcgctgctc ggtgcgctgc
     3661 agcaggatgc cgacgttgtg atcgtcgatc tcggctacga cgtatgcccg ccggggcatc
     3721 ttggggtcga accagccgag gaagtcgatc ttctcgaagt cgatctcgtc gaagtcgagt
     3781 ggcaggttca gggatgagac ctccttgcgg gaggcgttgc ggaaggaggc gcggatggcg
     3841 cgctcgtcga tgggacgcat gaggaagtgc tttctgtgtc agcaccgccg atgacggcgg
     3901 agaggtgggt cgcgcccgga tgccgggtgc gaccggagat gagggcggtc ccggaagccg
     3961 ggtacgccga gagagaaggg gagcccggat gccgggcatg acgcttctcc ggtgacggga
     4021 gttccgccgc cggatcgctc tcagcctctg ccgtcggcgc gtgacgcgcc gacgatcacc
     4081 tctcgcgcca cgaaggcgcc ctctgcgaac agcacgtcgc ccagtgtacg ccgcctgcgc
     4141 tcccgctact ctggacccgg aagagggggg ctcttgatgg cgacgaagcc gggcgcaggg
     4201 atgccgctgc gcgactatcg catccaccgg tacgtgaacg cgttctgccc acgctgccac
     4261 gaggagaagc cggatcagcc gctcagtgag gtgcgccgcc tgtcgggctg gctcgccgac
     4321 cgcgacggca cggtctggct ggagcgcgga tgccccgagc acggactcgt ctccacgctc
     4381 tacgacgaga acgccgacat cctgcggtac ctcgagcagt ggacggcgcc gaccaaggtg
     4441 cacacgcccg acacaatggg caacttcaag cccgtgccgc aggcgtacga ggacggcctg
     4501 ccgcagatgc agacgcagca cacctgcatc ctgctggagg acatcaccga ccactgcaat
     4561 ctgaagtgcc ccacctgctt cgcggagtcg agccccgcgg catccgccgt cgccccgctc
     4621 gccgaggtgc tggcatccgt cgacgccaag ctcagccgcg agaacgaccg catcgacgtg
     4681 ctcatgctct ccggcggcga gccgacgctg tacccctggc tggagcagct catcgagcac
     4741 ctggtcgcgc ggcccatcgt gcgcatcctg ctgaactcga acgggctgcg gatcgcgaac
     4801 gacgacgagt tcgtccagtt cctgaagaag caccgcgagc gcgtcgaggt gtatctgcag
     4861 tacgacggcg aggaggcggc ctccgtcgcc ttccaccgcg gcgcggacat ccgccggttc
     4921 aaggagcggg cgttggaacg gctgtcggat gccggtatct tcacgaccct gaccatgacc
     4981 gcggcactgg gcgtcaacga ccacgagatc ggcaacgtca tccggcgagc gcaggcgacg
     5041 ccgtacgtcg gaggggtgac gatccagccg gtgttcggat cgggacggaa ctcggggatc
     5101 gacccgaacg agcgactcac ccacaccggc gtgctcgccc ggatcggccc gcagaccaag
     5161 ggcgagatca cctggcgcga cctgaccgcg ctcccctgca gccacccgca ctgctgctcg
     5221 gtcggctact tcctgaagga cgactccggc gattggaagt cactcgtggg gttgatcggg
     5281 cacgagaggc tcaaggagtt cctcgacctc aaccccgacc tgatcgccaa ccgcatcgcg
     5341 gactcgaacg tgaacaaggc gatacgcgac gtggtgaaga actcgctgct ggatctgctc
     5401 agcgagcagt cgtcgctctc gcacccctcg atgggcgata tctggcgcga catctgcgtg
     5461 aactgcgacc tgggcgtggg cacactcgcg acgcttgccg cgtcgaagct gccgggtcag
     5521 cacaagcgga tgcgggccat gctcgccgag cgggtgctgc gcatcacggt caagccgttc
     5581 atggacatca acaccatgat cgaggagcgc ctcacgcagt gctgcgtgca cgtggccacg
     5641 gtgaacgagc agacgaacgc gcaccagtgc gccccgttct gcgcgctgca ggcgtgggcg
     5701 ccgctcagcc gcacgcgcat ctccacggcg ctcgggcgtc ccgcgtcgtc gatgaccgag
     5761 gtgcgcgaga cgggcgggcg cacccggctt ccggtggtgc aggcgtcatg acctgggccc
     5821 ccgagcctgt cgaagggcac ccgctcaacc agttcgaccc cgcagtcggt catcccgcgc
     5881 cgccgttcga cccgctgcgg ctgtgcgtgt tcaccacgat cgcggcgatc tcgtgcgtcc
     5941 tggggccggc ggccgtgctc gtgttcgcgc tggtcgcgat cgccggctat gcgaaggccc
     6001 gccgtggcgg gctgctgcgg tcgcgctgca agctgggaga cacccgcctg gtgctggcgt
     6061 acctgtcggt gctcgcggtg gcgtcggcgg tcggcatccc gttctgggtg atgctctgga
     6121 tgcggatgct gggcggctga gatgttcccc accctgcatg acatcgcccc ctggctgccg
     6181 cggctggaca cgcattcggt gttcgtcgcg ctgggcatgc tggccgccat cgcggtgttc
     6241 ctcatcgaga agcgccggcg cggggtgcgc gacccgcgga tcgtctacct ggtgctgggg
     6301 gcgatggcgg gagccgcgat catggcgcgg ctcggcacct gggcgcagca tctggacccg
     6361 tcgaagaacc tcaacctcgg tgagcagctg ctgcggggga acgcctcggt gctgtcggcg
     6421 ctcgtcggcg cgtggctggg tgtgcacatc gccaagaagc tctgccggta cccgggtcgc
     6481 accggcgacc tgttcgcgcc ggccgtcgcg ctggcgatgg cgatcgggcg gatcggatgc
     6541 ctgctcaccg agcagcccgg gacgccgacg ggcgcggcct ggggcatcgt gctggacgcc
     6601 gacgccgcgg ctcgtacggg ctcaccggcc ggcgtcccgc tccacccgag cttcgtgtac
     6661 gagatcgtct tccacgcggt cgccttcgcg gtgctgtggt tctggctgcg gcatcgtccg
     6721 atcgccgccg gtgagacgct cacgatctac atcgccgggt acgcggtgtt ccgcttcctc
     6781 atcgagttcg tccgcggcaa cgatgtcgcg tggatggggc tgacgagacc gcagctgttc
     6841 ctggcggtca cgatcccgct gctgctggcg cggatcatct ggatgctgcg gcgcgggcgc
     6901 ttccgcgccg agcctggtgt ggagaggaag gtgcctgatg gacagccagc ctgaagaccc
     6961 tgcgcagaat gcgccgctgc ctccggacgg aggatcggcg acgccgcctg ttccgccatc
     7021 gtcggagggc gccccgtcgg cgcagcccac ggccgccgct ccctacatt
//
LOCUS       DATMSX010000443         5830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_120713,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000443 DATMSX010000000
VERSION     DATMSX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120713"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VN041_14220"
     CDS             complement(<1..1057)
                     /locus_tag="VN041_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU30235.1"
                     /translation="MTSRIEDAFRADAGRILGAVTAYTGDLQLAEDAVQDAFLRAIAQ
                     ERSGVELRAPSAWITTAARRIAVDAIRRERTAARALPTLAADARIVDDADLHAFSFTG
                     DERLELILTVCHPDLPEETRLALALRFACGVPTRDVAAMLLVSEATMAARLTRAKKRL
                     HDSEIRFAVRDDEQVRTRMPDALSVIALLYTTGYAAPTSDEGRVLCRDAVDLARDALR
                     IAGDDAEAQGLLALVLLTEARRPSRVDPSGELVALAEADRTLWDRRLIAEGERLATDA
                     LQHGTGRFALQAGIAGLHAIAPTWEATDWPSIARFYDGLVEQWPSPSARLARIVARGY
                     AEGIGAEGVRLELDADEV"
     gene            complement(1071..1436)
                     /locus_tag="VN041_14225"
     CDS             complement(1071..1436)
                     /locus_tag="VN041_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWU30236.1"
                     /translation="MSDEYMIIIHEPEWDYSTRTPAQLDADLEGHKAFQAAVAAAGQR
                     LTAGVPLRAQSEAFRIRPSAEGPVYTDGPFGETREVVTGFYTFTADSVEQAKELAALV
                     PTGGWLDVHPVLDMTAMRG"
     gene            complement(1616..3235)
                     /gene="serA"
                     /locus_tag="VN041_14230"
     CDS             complement(1616..3235)
                     /gene="serA"
                     /locus_tag="VN041_14230"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWU30237.1"
                     /translation="MSNQSASKPVVLIAEVLSPATIEALGPDFEVRNVDGTDREALFA
                     ALAEADAVLVRSATKVDAEALSHAPKLKVVARAGVGLDNVDIKAATTAGVMVVNAPTS
                     NIVSAAELTIGHILSLARRIPAAHASLSGGAWKRSSFTGAELFEKTVGIVGLGRIGAL
                     VAARLNAFDMRVIAYDPYVTSARAQQLGVQLVSLDELVAEADFLTIHMPKTPETTGMI
                     AAEQFAAMKPTAFVVNVARGGLIDEADLHEALVSGEIAGAGLDVFTSEPPVEGSTAQA
                     LLSLPNVVVTPHLGASTEEAQEKAGVSVARSVRLALGGDLVPDAVNVAGGVIDPYVRP
                     GIALVEKLGQIFSALSQSPLTSLDVEVHGELNDYDVSVLKLAALKGVFTNIVSDNVSY
                     VNAPLLAEQRGVAVRLLQDDISEEYRNVITLRGALSDGSQLAVSGTLTGPKQIEKLVS
                     INGHAIELPIEKHHVVMTYTDRPGIVAVYGQKFGEAGINIAGMQIAREAAGAQALSVL
                     TLDSPVTDELLADVSAAIDADLFRQIEITEV"
     gene            complement(3367..3720)
                     /locus_tag="VN041_14235"
     CDS             complement(3367..3720)
                     /locus_tag="VN041_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30238.1"
                     /translation="MSGNASVDATQHGGRVRSALRGFLTGLGGLAYAFVIWTAVFYAI
                     QLSVGGMSGYGWVVLLLPAVVTAAVFAVVLVLTRRRSTGALVLVLLAGLGLSAVFLLN
                     TFSYVIRNLAALTGS"
     gene            complement(3717..4223)
                     /locus_tag="VN041_14240"
     CDS             complement(3717..4223)
                     /locus_tag="VN041_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWU30239.1"
                     /translation="MRSDGDDDALSWDGDDALEARRPEMRPRRSRPQETPRPASAPKH
                     EDALSVASDPSSIRTTDAGTDDEPQGLSTVTLVTLGILGGVYLLYTVGWVIGGVGMQA
                     PVMFTLPAFLYQAALWLAVLAPALWFIAVLVVTRGSKDWVRIVWLIAGAALLVPWPFV
                     VSGGGAAL"
     gene            4296..5222
                     /locus_tag="VN041_14245"
     CDS             4296..5222
                     /locus_tag="VN041_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30240.1"
                     /translation="MAESKNKDVRPDAEAPAERTWVPTAEAKKKATTFRWIAAVLWLV
                     AIVGEAVGIFWLLKQRVVTGADGTLVRDNDTGLLREEGVTTTFPQWAFITLLVLLVVI
                     AALSITGSILWKKANHLDPAKKSDTVRFFVQNQLGAIIAIIAFVPLVILIFLNRDMDK
                     GQKTTAGIVGIVLAALAVFIGVDFNPASVEKYTADQSTVIQLLGKDQVTWVAGGSVYH
                     VCDQVSDIQTGSEIQTGTTAEAVAAHKERLTLKFASELEKCGLPVPENASEIADALRQ
                     IQGGTTVTLPAPVWGEGVKPPVEVKEAAPAAN"
     gene            complement(5286..>5830)
                     /locus_tag="VN041_14250"
     CDS             complement(5286..>5830)
                     /locus_tag="VN041_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HWU30241.1"
                     /translation="GGFVYDADEVALVAADVREIVRRGAGGVVVGAMTREGRVDRDAM
                     RRWIDAAEGAEVSFHRAIDVAPDPVAIMDELIELGVHRVLTSGGATRSVHGIGVIGDL
                     VARSAGRVQVMPGGGVHVEDIARLIALGVDAVHLSARVDSGDAAPSGPGGGIIGFDAT
                     DSSIVAAARAALDRAVRSAA"
BASE COUNT          912 a         2042 c         2041 g          835 t
ORIGIN      
        1 gcacctcgtc ggcgtccagc tccagccgga ccccctcggc tccgatcccc tccgcgtaac
       61 cgcgggcgac gatccgcgcc agccgtgccg acggcgaggg ccactgctcg accagcccgt
      121 cgtagaaccg tgcgatcgac ggccagtccg tcgcctccca cgtcggcgcg atcgcgtgca
      181 gcccggcgat gcccgcctgc agcgcgaagc gacccgtccc gtgctggagt gcgtccgtgg
      241 ccagccgctc cccctccgcg atcagccggc gatcccacag cgtccggtcc gcctctgcga
      301 gggccacgag ctcacccgag ggatccaccc gagacggtcg ccgcgcctcg gtgagcagca
      361 cgagggcgag cagtccctgt gcctcggcgt cgtcgcccgc gatgcgcagc gcgtccctgg
      421 cgaggtcgac cgcatcacgg cagagcacgc gcccctcgtc gctcgtaggc gccgcatagc
      481 ccgtcgtgta gagcagggcg atcactgaca gggcgtcggg catgcgcgtg cgcacctgct
      541 cgtcgtcccg cactgcgaat cggatctcgg agtcgtgcag gcgcttcttg gcgcgggtca
      601 gccgcgccgc catagtcgcc tccgacacca gcagcatggc cgcgacgtcc cgggtcggaa
      661 caccgcacgc gaaccgcagt gcgagtgcga gcctggtctc ctcgggcagg tcgggatggc
      721 agaccgtgag gatgagctcc agcctctcgt ccccggtgaa ggagaacgcg tgcagatcgg
      781 cgtcatcgac gatccgggca tccgccgcca gggtgggcag ggcgcgggcg gcggtgcgct
      841 cccgccgaat cgcatcgacg gcgatacggc gcgccgcggt ggtgatccag gccgacggcg
      901 cgcgcagctc cacaccggac ctctcctgcg cgatggcccg gaggaaggcg tcctgcaccg
      961 cgtcctcggc cagctgcagg tcgccggtgt acgccgtgac cgccccgagg atgcgtcccg
     1021 cgtctgcgcg gaacgcatcc tcgatgcggc tcgtcatcgt cacgcctcgg tcagccccgc
     1081 atcgccgtca tgtcgagcac cggatgcacg tcgagccagc ctccggtggg gacgagagcg
     1141 gcgagctcct tcgcctgctc gaccgagtcg gcggtgaagg tgtagaaccc ggtgacgacc
     1201 tcgcgcgtct cgccgaacgg gccgtccgtg tagacgggcc cttcggcgga gggccggatg
     1261 cggaacgcct cggactgcgc gcgcaggggc actccggccg tgaggcgctg ccccgccgcg
     1321 gcgacggccg cctggaacgc cttgtgaccc tcgaggtcag cgtccagttg cgccggagtg
     1381 cgcgtggagt agtcccattc gggctcgtgg atgatgatca tgtattcgtc ggacatcgtg
     1441 tctccttcgt cgtgcgggtg cgtgacccgc tgaggtgtcg aacaccgata tgacgtgcgg
     1501 gcgcccggga aatcgacagc ccggctggat tctttcagaa cttcttccgg ggcggaacgg
     1561 cgaacgaccc gccctcggaa gaggacgggt cgttcgggac ctcatatggc gcggatcaga
     1621 cctcggtgat ctcgatctga cggaagagat cggcgtcgat cgcggcgctc acgtcggcga
     1681 gcagctcgtc ggtgacgggc gagtcgagcg tcaggacgct gagcgcctgg gcgcctgccg
     1741 cctcacgggc gatctgcatg ccggcgatgt tgatgcccgc ctcgccgaac ttctggccgt
     1801 acacggccac gatgcccggg cggtcggtgt aggtcatcac gacatggtgc ttctcgatgg
     1861 ggagctcgat cgcgtgcccg ttgatggaca ccagcttctc gatctgcttc gggccggtca
     1921 gcgtgcccga cacggccagc tggctgccgt cggagagggc gccgcgcagc gtgatgacgt
     1981 tgcggtactc ctcgctgatg tcgtcctgca gcaggcgcac ggccacgccg cgctgctcgg
     2041 ccaggagcgg tgcgttcacg tacgagacgt tgtcgctgac gatgttggtg aacacgccct
     2101 tgagcgcggc gagcttgagc acgctgacgt cgtagtcgtt cagctcaccg tgcacctcga
     2161 cgtcgaggct ggtcagcggc gactgcgaca gggcggagaa gatctgaccg agcttctcga
     2221 ccagggcgat accggggcgc acgtacgggt cgatcacgcc accggcgacg ttcacggcat
     2281 ccggcaccag gtcgccgccg agcgcgagac gcacggagcg ggcgacggag acgccggcct
     2341 tctcctgagc ctcctcggtg ctggcgccca ggtgcggggt gacgaccacg ttcggcaggc
     2401 tcagcagagc ctgcgcggtg ctgccctcga ccgggggctc ggaggtgaag acgtcgagcc
     2461 cggcgccggc gatctcgccg gagacgaggg cctcgtgcag atcggcctcg tcgatcaggc
     2521 cgccgcgggc gacgttgacg acgaaggctg tcggcttcat ggcggcgaac tgctccgcgg
     2581 cgatcatgcc cgtggtctcg ggggtcttcg gcatgtggat ggtgaggaag tcggcctcgg
     2641 cgaccagctc gtcgagcgac acgagctgca cccccagctg ctgcgcacgc gcgctcgtga
     2701 cgtaggggtc gtacgcgatc acgcgcatgt cgaaggcgtt caggcgggcg gcgacgagag
     2761 cgccgatacg gccgagcccc acgatcccga cggtcttctc gaagagctcc gcacccgtga
     2821 acgagctgcg cttccatgcg ccgccggaga gcgaggcgtg cgcggcgggg atgcggcgcg
     2881 cgaggctcag gatgtggccg atcgtcagct ccgcggccga gacgatgttg gaggtcggtg
     2941 cgttcaccac catgacgccg gcggtcgtgg ccgccttgat gtcgacgttg tcgaggccga
     3001 cgcccgcgcg tgcgacgacc ttcagcttgg gcgcgtgcga gagcgcctcg gcgtcgacct
     3061 tcgtcgcgga gcggaccagc acggcatccg cttcggcgag ggcggcgaac agcgcctcgc
     3121 ggtcggtgcc gtcgacgttg cgcacctcga agtccggccc gagcgcctcg atcgtggcgg
     3181 gcgagaggac ttcggcgatg agaacgacgg gcttggatgc ggactggttc gacacaggta
     3241 tccttcgggg cagcacacgg tggaggggca gtcgatgcgc cgcacgccgg cgggggcgcg
     3301 atcgggacac actttacccg agcggcatcc acgctcccga ccgtgtgacg ccgggagtcc
     3361 ctgtggtcag gagcccgtca gcgccgccag gttgcggatc acgtagctga acgtgttcag
     3421 caggaagacc gcgctgagcc ccaggcccgc gagcagcacc aggacgagcg ccccggtcga
     3481 acgcctccgg gtgaggacga ggacgacggc gaagacggcc gccgtgacca ccgcgggcag
     3541 cagcaggacc acccacccgt aacccgacat ccctccgacc gagagctgga tcgcgtagaa
     3601 cacggctgtc cagatgacga acgcgtaggc gaggccgccg agtccggtga ggaacccgcg
     3661 cagggcactg cggacgcggc cgccgtgctg cgtggcgtcg accgatgcgt tcccgctcac
     3721 agcgcagcac ctcccccgga gacgacgaac ggccagggca cgagcagggc tgcgccggcg
     3781 atcagccaga cgatccgcac ccagtccttc gaaccgcgcg tgaccacgag gacggcgatg
     3841 aaccacagcg ccggcgccag cacagcgagc cacagcgcgg cctggtacag gaatgcgggg
     3901 agcgtgaaca tcacgggcgc ctgcatcccc actccgccga tgacccaccc gacggtgtac
     3961 aggaggtaga cgccgccgag gatgccgagc gtcaccaggg tcacggtgct gaggccctgc
     4021 ggttcgtcat cggttccggc atccgtggtc cggatcgagg acggatcgga tgccacagaa
     4081 agcgcgtcct cgtgcttcgg cgcggaagcg ggtcgcggcg tctcctgcgg ccgcgacctg
     4141 cggggtcgca tctccggacg gcgcgcttcc agcgcatcgt ccccgtccca gctgagggcg
     4201 tcgtcgtcgc cgtcggatct catcgttcca gcgtaccgac gcgcgacccc gctccccact
     4261 atgctcaggg cgacaccact tccggggagg aaaccgtggc agagagcaag aacaaggacg
     4321 tccggcctga tgccgaggcg cccgccgagc gcacctgggt gcccacggcg gaggcgaaga
     4381 agaaggcgac aacattccgg tggatcgcgg cggtgctgtg gctggtcgcg atcgtcggcg
     4441 aggcggtcgg catcttctgg ctgctgaagc agcgtgtggt cacgggcgcc gacggcaccc
     4501 tggtgcgcga caacgacacc gggctgctgc gcgaggaggg cgtcaccacg accttcccgc
     4561 agtgggcgtt catcaccctg ctcgtgctgc tggtcgtcat cgcggcgctc tcgatcaccg
     4621 gctcgatcct gtggaagaag gccaaccacc tcgatccggc gaagaagtcg gacaccgtgc
     4681 gcttcttcgt gcagaaccag ctgggcgcga tcatcgcgat catcgccttc gtcccgctgg
     4741 tcatcctgat cttcctgaac agggacatgg acaagggtca gaagaccacg gccggcatcg
     4801 tcggcatcgt gctcgcggcc ctcgccgtct tcatcggcgt cgacttcaac ccggcgtccg
     4861 tcgagaagta caccgccgac cagtccacgg tcatccagct gctgggcaaa gaccaggtca
     4921 cctgggtcgc gggcggctcg gtgtaccacg tgtgcgacca ggtctccgac atccagaccg
     4981 gcagcgagat ccagaccggc accaccgccg aggccgtcgc ggcccacaag gagcgcctca
     5041 cgctgaagtt cgcctccgag ctggagaagt gcggcctgcc ggtccccgag aacgcctccg
     5101 agatcgccga cgcactgcgt cagatccagg gcggcacgac cgtcacgctt cccgctcctg
     5161 tgtggggcga gggcgtcaag ccccctgtcg aggtgaagga ggccgcaccc gcggcgaact
     5221 gacccgatcg cacgaagaag aggccgtgcc cggagctccg ggcacggcct cttcttcgtc
     5281 tcctgctagg cggcggagcg cacggcccgg tcgagcgcag cacgggccgc ggccacgatc
     5341 gaggagtccg tcgcatcgaa accgatgatc ccgccgccgg gtccgctcgg cgcggcgtcc
     5401 cccgagtcca cccgggcgga cagatgcacg gcatcgacgc cgagcgcgat cagtcgcgcg
     5461 atgtcctcga cgtgcacgcc gccgccgggc atgacctgca cacggccggc cgagcgtgcg
     5521 accaggtcgc cgatcacgcc gatgccgtgc acgctgcgcg tggcaccgcc gctggtgagc
     5581 acgcgatgca cgccgagctc gatgagctcg tccatgatcg ccacgggatc gggggccaca
     5641 tcgatcgcac ggtggaacga cacctcagcg ccctccgcgg cgtcaatcca gcggcgcatg
     5701 gcgtcgcgat ccacccggcc ctcccgtgtc atcgccccga cgaccacgcc acccgcgccg
     5761 cgtcggacga tctcgcggac gtcggccgcg accagggcga cctcgtccgc gtcgtagacg
     5821 aatcctcccg
//
LOCUS       DATMSX010000444         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_45096,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000444 DATMSX010000000
VERSION     DATMSX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45096"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VN041_14255"
     CDS             complement(<1..264)
                     /locus_tag="VN041_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30242.1"
                     /translation="MLFEGGHGSAVRVDVDVSRRDAVTVVAASEAPAHLAVAVAVGVG
                     AMLETGAVLGPVLAEAVAVTKVVLSGVAPVAEAVAAIGAAATGD"
     gene            461..1339
                     /locus_tag="VN041_14260"
     CDS             461..1339
                     /locus_tag="VN041_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU30243.1"
                     /translation="MSSPVLSILPTPTSSPSIDALERVLGEVAISIRSQHRILPDADQ
                     AVVLDGLSLVYVLRGSIGTPTDAGLPDDLRAGDALLVADRSLTLRIPAGSGVLVSRLA
                     WADSAAHLSALLPDTAWIQGFDELEPAATALAHHMGGDAPSVCERSGDLVICRMMATT
                     LLQSVIRAWSSVGCAPQGWPSRSNDPFLDRVVSAIDSDPGREWSVEQMASVSALSRTV
                     FAERFRLAFGISPAQYVAEVRMRAARALLIDGRGVSEVSRELGYGSDEGFSRAFRRHT
                     GMTPSAFRMRGALASA"
BASE COUNT          199 a          597 c          476 g          234 t
ORIGIN      
        1 gtccccggtc gccgcggctc cgatcgcggc cacggcttcg gccacgggcg ccactccgga
       61 gagcacgact ttggtcacgg ccacggcttc ggcgagcacg ggtccgagaa ccgccccggt
      121 ttcgagcatc gcgccgacgc ccacggccac agccaccgcg agatgggccg gggcttcgct
      181 cgcggccacc accgtcacgg catcccggcg actcacatcc acatccacgc gcacggctga
      241 gccgtgccca ccctcgaaaa gcatccgcgc ccatccggtc cggatgcttt tcgccgttcc
      301 ccgcgcctcc caccgacgcc tttccctctc gccctgatcg agccggaggg aaagcgcccg
      361 gtatccggag tcctcaccca ccgattccca ccccgacgaa tcgtggcctc gaagtcgtcc
      421 ccaccggaat agtcacgcgc acaggcaggt tgccctgttc atgagcagtc ctgtcctgag
      481 catcctgccc acgcccacat cctcgccgtc gatcgacgcg ctggaacggg tgctcggcga
      541 ggtcgcgatc agcatccgct cgcagcatcg catcctgccc gacgccgacc aggccgtcgt
      601 cctcgacggc ctctccctcg tgtacgtgct gcgcggcagc atcgggacgc cgaccgatgc
      661 cgggctcccg gacgaccttc gcgccggtga cgcgctgctc gtcgccgacc gctcgctgac
      721 tctgcgcatc ccggcgggtt ccggtgtgct cgtgtcgcgg ctcgcctggg ccgacagcgc
      781 cgcgcacctc agtgcgctgc tgcccgacac cgcctggatc cagggcttcg acgaactcga
      841 gcccgccgcg acagcactcg cccaccacat gggcggcgac gcgccgtccg tctgcgagcg
      901 cagcggcgac ctcgtcatct gccggatgat ggcgaccacg cttctgcagt ccgtgatccg
      961 cgcgtggtcg agtgtcggct gcgcaccaca gggctggccg tcgcggtcga acgacccgtt
     1021 cctggatcgc gtggtcagcg cgatcgactc ggacccgggc cgtgagtgga gcgtcgagca
     1081 gatggcatcc gtcagcgctc tgtcccgcac ggttttcgcg gagcgcttcc gcctcgcctt
     1141 cgggatctcc cccgcgcaat acgtcgccga ggtgcgaatg cgggcggccc gcgccctgct
     1201 catcgacggc cgtggcgtga gcgaggtctc ccgcgagctc ggttacggct cagacgaggg
     1261 cttcagccgt gcgttccggc gccacaccgg gatgacgccg tcggcgttcc gcatgcgcgg
     1321 cgccctggca tccgcctgat ccgccaccct gctcgcacgc cgtgtcccag aaacggcggg
     1381 tgctgccgcg tctgagccgc cccttctgag acacggctcg cagcgtgtcc cagaagtggc
     1441 ccgaccgggc ggctcgcacc cgcctcttct gggacacgcc gccggccgtg gcccctggcg
     1501 cggcat
//
LOCUS       DATMSX010000445         5564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_167399,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000445 DATMSX010000000
VERSION     DATMSX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167399"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..817)
                     /locus_tag="VN041_14265"
     CDS             complement(230..817)
                     /locus_tag="VN041_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWU30244.1"
                     /translation="MSGYTIPNVIAQNPRGDRIMDVYSHLLSERIVYLGTEVDAGVAN
                     TIIAQLLHLDADSPDSDVQLSVNCAGGDPSAALAIYDTMQHIRPRVTTTCVGQAIGVG
                     ALLVAAGAPGMRSALPHSRIVLHQPAAQTRGAVPDLILAADEVVRVRSEMEQVLAHHT
                     GRTVEALRADTDRDRVFTAQTALDYGLIDQVLAPR"
     gene            complement(814..1494)
                     /locus_tag="VN041_14270"
     CDS             complement(814..1494)
                     /locus_tag="VN041_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWU30245.1"
                     /translation="MTDDKPIPQFGAEARRALFHERVLVLDGPLDDDNGTLLMTQLLS
                     LAAEDPVADIALWIHSPGGSVPAMLGIRDIIHAIPNDVATVALGLACSAGQFLLSAGT
                     KGKRRALAHSRILLHQGSSGIGGSAVEIEVQADDLRHMRDTVIGLIADDTGQPRERVF
                     EDSLHDHWYTADEALEYGFIDGVVTSLADVFPRRRARIGLGADGADAALGRAASAAPT
                     DPKKEESR"
     gene            1624..1962
                     /locus_tag="VN041_14275"
     CDS             1624..1962
                     /locus_tag="VN041_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWU30246.1"
                     /translation="MTESAERIATNLRKGVLEFCVLGLLAAGERYGLELASELQSRGL
                     IAGEGSLYPLLARMRDGGLVDTRTEAVGGGRPRKYYTITPAGRDQLTTFTAVWRAVGH
                     EVDTIIEGAS"
     gene            1959..2930
                     /locus_tag="VN041_14280"
     CDS             1959..2930
                     /locus_tag="VN041_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30247.1"
                     /translation="MTTEDEYLRSVERMLRRIDAAHRASVLDDLRGHFADAEDAGRPV
                     EETIRGLGTPEEIAERASEEFGGSDEVDAAAERAWRVLQGAATVSALIIGVAVGFIVP
                     THTIATSEGGDSVLQHLTILQNDGLWVALLLLIPAVFAAVPLVVARAARIAVAVICGM
                     LLIAVSLIAVFTVGEFFLPVLLLSWASLIVWARLRGSGFGRGWRIAGGVLAAAPVLLS
                     IGSLVGPFGFEFGQRRRYADYSEVGSGPSIGIEAPGWLLVGAIVALAVLIAIGYRWAG
                     WALAAVGFVALITGLVAGSLLALLFVWLGGWWLTIGLAHAVTASRRA"
     gene            3088..4008
                     /locus_tag="VN041_14285"
     CDS             3088..4008
                     /locus_tag="VN041_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWU30248.1"
                     /translation="MPSNLRWMRLRPQELALIAITAVWGSTFLLVHWAMQHSDPWFFV
                     GLRFLVAGLVSILIFRHALRGMRWTDIAAGASIGVMIYGGYGLQTLGLQTINSSTSAF
                     LTALYVPMVPFAQWIFFRKRPPAMAFLGAGLAFVGLLLIAGPDAFSMSLGVGETVTLM
                     STIPIVGEIILISVFAGRVDLGRITIVQLFTAGLLGLATMPFVGESLPAFDWAWVIAG
                     VGLGVASCIIQLTMNWAQKSVSPTRATIIYSGEPVWAAVIGRIAGERLPAAALVGGLL
                     VVLGILASELRIVRRRSRGGSGEGHTPIRG"
     gene            4061..>5564
                     /locus_tag="VN041_14290"
     CDS             4061..>5564
                     /locus_tag="VN041_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWU30249.1"
                     /translation="MTRDATAATNAVREMRDFLFAHGDDYDGARAGFAWPQVDSFNFA
                     LEWFDVVAGENPDRPAVQIVSADLSVQSWSYGRLSERSDQVANWLRALGIRRGDHLIV
                     MLNNTIELWEIMLAITKIGAIAIPTSTLLSASDLAYRIDHGRARAVVALGGLADRVED
                     ADVLRIAVGDATAGWHDFAASADAAAVFEPDGPTPASDTALLYFTSGTTSRPKLVQHT
                     HVSYPVGHLSTMWWLGVRPDDVHLNISSPGWAKHAWSSFYSPFLAEATVFVYNYDRFD
                     ANTLMQVMDTHHVSTFCAPPTVWRMLIQADLSRLTNPPRELVGAGEPLNPEVINRVRE
                     AWGSTIRDGYGQTEMTACVGNSPGQLVKDGSMGRALPGYPVVLLDPVTGEVTDAEGEI
                     ALDLSEAPLGLMSGYYEDPEKTAESRAGGFHHTGDIAARDADGYLTYVGRADDVFKAS
                     DYKISPFELESALLEHELVVEAAVVPSPDPTRLAVPKAYVILHGSAGSGAE"
BASE COUNT          771 a         1965 c         1927 g          901 t
ORIGIN      
        1 accggcggag cgttcgccct cgccgcctga ggcatccgac cgcgcgtccc ccgccatccg
       61 cggcatcccg gatccccggt ccgtcgcggg cgcggttcca ccaccgcagg gacacctgca
      121 cgacgcggga acggatgcac gacgatcggg gcgctcccgt cgtgcatccg tacccggatc
      181 gtgcatccgt acccggccgc aggtcgccac cagcacccgg cggatgcgat cagcgcggcg
      241 cgagcacctg gtcgatcagg ccataatcca gcgccgtctg cgcggtgaag acccggtcac
      301 ggtcggtgtc ggcacgcagc gcctcgaccg tgcgcccggt gtgatgcgcg agcacctgct
      361 ccatctcgga gcgcacgcgc accacctcgt ctgcggcgag gatcaggtcg ggcacggccc
      421 cgcgggtctg cgccgccggc tgatgcagga cgatgcgtga gtgcgggagc gcggatcgca
      481 tgccgggggc gccggctgcc acgagcaggg cacccacgcc gatcgcctga cccacgcacg
      541 tggtcgtgac ccgcgggcgg atgtgctgca tcgtgtcgta gatcgcgagg gcggcgctcg
      601 ggtctccccc ggcgcagttc accgagagct gcacgtcgct gtcggggctg tccgcgtcca
      661 ggtgcagcag ctgggcgatg atcgtgttcg cgaccccggc atccacctcc gttccgaggt
      721 agacgatccg ctccgagagc aggtgcgagt agacgtccat gatccggtcg ccgcgcgggt
      781 tctgggcgat cacgttcggg atcgtgtagc cgctcatcgc gactcctcct tcttcgggtc
      841 cgtgggggcg gcactcgccg ccctccccag agccgcatcg gcgccgtcgg cgcccagccc
      901 gatccgcgcc cggcgccgcg ggaacacgtc ggcgagcgag gtgacgacgc cgtcgatgaa
      961 gccgtactcc agcgcctcgt ccgcggtgta ccagtggtcg tgcagcgagt cctcgaacac
     1021 gcgctcgcgc ggctgcccgg tgtcgtcggc gatcaggccg atcacggtgt cgcgcatgtg
     1081 ccggaggtcg tcggcctgca cctcgatctc gaccgcggaa ccaccgatgc ccgacgaccc
     1141 ctgatgcagc aggatgcggg agtgcgccag cgcccgccgc ttgcccttcg tgcccgccga
     1201 gagcaggaac tgcccggcgc tgcaggccag tcccagtgcg acggtcgcga cgtcgttggg
     1261 gatcgcgtgg atgatgtcgc ggatgccgag catggccggc accgatccgc cgggcgagtg
     1321 gatccagagc gcgatgtcgg cgaccggatc ctcggcggcc aggctgagta gctgggtcat
     1381 gagcagggtg ccgttgtcgt cgtcgagcgg tccgtcgagc acgaggacgc gctcgtggaa
     1441 cagcgcgcgg cgcgcctcgg cgccgaactg cgggatgggc ttgtcatcgg tcatggctcc
     1501 agcgtgcgct ggacgggtcg ccgctgtcgc gggaatccgc cccgggcgga tccgccgtgg
     1561 gcggaggggt cgaatctaaa accttgcatt gcaatacttg ccatgcatga ttagtctgtt
     1621 ggcatgacag aaagcgccga gcgcatcgcc accaacctcc gcaagggggt gctggagttc
     1681 tgcgtgctcg gcctgctcgc cgccggcgag cggtacggcc tggaactggc atccgagctg
     1741 cagagccgcg ggctcatcgc gggagaggga agcctctatc cgctgctggc gaggatgcgc
     1801 gatggcggac tcgtagacac ccgcaccgag gcggtcggcg gcgggcgacc gcggaagtac
     1861 tacacgatca cacccgccgg gcgcgatcag ctgaccacct tcactgcagt ctggcgagcc
     1921 gtcggccacg aggtcgacac catcatcgag ggggcatcat gacgaccgaa gacgaatacc
     1981 tgcggtcggt ggagcggatg ctgcgtcgca tcgacgcggc gcaccgcgct tctgtgctcg
     2041 atgacctgcg cgggcatttc gcggatgccg aggacgccgg ccggccggtg gaagagacca
     2101 tccgcggcct gggcaccccg gaggagatcg cggagcgcgc atccgaggag ttcggagggt
     2161 ccgacgaggt ggacgccgcg gccgagcgcg cctggcgggt actgcagggt gcggccaccg
     2221 tgagcgccct gatcatcggc gtcgctgtgg gattcatcgt gccgacgcac acgatcgcga
     2281 cctccgaggg tggggactct gttctgcagc acctgacgat cctgcagaac gatggcctgt
     2341 gggtcgccct tctcctcctg atccccgccg tcttcgccgc cgtccccctg gtggtcgcgc
     2401 gagccgcgcg cattgctgtc gccgtcatct gcgggatgct gctgatcgca gtcagcctga
     2461 tcgcggtgtt caccgtcggg gagttcttcc tgcccgtcct cctgctgtcg tgggcctcgc
     2521 tgatcgtgtg ggctcgtctg cgcggatcgg gcttcgggag gggctggcgc atcgcgggcg
     2581 gtgtgctcgc cgcggcgccg gtgctgttgt cgatcggttc gctcgttggc ccgttcgggt
     2641 tcgagttcgg ccagcgtcgc cgctacgcgg actacagcga ggtcggcagt ggcccgtcca
     2701 tcgggatcga ggcaccgggc tggctcctcg tcggcgccat cgtcgcgctc gcggtactga
     2761 tcgccatcgg ataccgctgg gcggggtggg cactcgccgc tgtcggcttc gtcgcactca
     2821 tcaccggcct cgtcgcaggc tccctcttgg ccctcctgtt cgtctggctg ggcggctggt
     2881 ggttgaccat cggactcgcg cacgccgtaa cggcatcccg ccgcgcctga ttccagcatc
     2941 cgcccccccc gatcccggca tccgaggaac acctgcacga tccgggaaca cgtgcacgac
     3001 cggatcgcgg gatcgaccgt gcatccgttc ccggatcgtg catccgttcc cgcgtctgca
     3061 cccctcccgg atgcgggaga atcggctgtg ccctcgaatc tccgctggat gcggctgcgc
     3121 ccgcaggaac tcgccctcat cgccatcacg gcggtctggg gcagcacgtt cctgctcgtg
     3181 cactgggcga tgcagcactc cgacccctgg ttcttcgtgg ggctgcggtt cctcgtggca
     3241 gggctggtca gcatcctgat cttccgtcac gcactgcgcg ggatgcgctg gaccgacatc
     3301 gccgcgggcg cctcgatcgg cgtgatgatc tacggcggct acggtctgca gaccctcggc
     3361 ctgcagacca tcaacagcag cacctcggcg ttcctcacgg cgctgtacgt gccgatggtg
     3421 ccgttcgcgc agtggatctt cttccgcaag cgcccgcccg ccatggcctt cctcggcgca
     3481 gggctcgcct tcgtcggact gctgctgatc gccgggccgg acgcgttctc gatgtcgctg
     3541 ggtgtcggcg agacggtgac gctgatgagc accatcccga tcgtcggcga gatcatcctc
     3601 atcagcgtgt tcgccggtcg cgtcgacctg gggcgcatca ccatcgtgca gctgttcacc
     3661 gcgggcctgc tggggctcgc gaccatgccc ttcgtcggcg agagcctccc cgccttcgac
     3721 tgggcgtggg tgatcgccgg cgtcggcctg ggcgtcgcga gctgcatcat ccaactgacg
     3781 atgaactggg cgcagaagtc ggtctccccc acgcgcgcca ccatcatcta ctcgggcgag
     3841 ccggtgtggg ccgccgtgat cggccgcatc gccggggagc gcctgcccgc ggccgcgctg
     3901 gtgggcggcc tgctggtggt gctgggcatc ctcgccagcg agctgcgcat cgtgcggcgc
     3961 cgcagccggg gcggatccgg agaaggccat acccccattc gggggtgaac tcccccgctc
     4021 ttcgggtcga agatggaggg atcgagcaag ggagcctgag atgacacgcg acgccaccgc
     4081 cgccacgaac gccgtccgcg agatgcgcga cttcctcttc gcgcacggcg acgactacga
     4141 cggcgcccgc gccgggttcg cctggccgca ggtggactcc ttcaacttcg cgctggagtg
     4201 gttcgacgtg gtcgcgggcg agaaccccga ccgcccggcc gtgcagatcg tatcggccga
     4261 tctgtccgtg cagagctgga gctacggccg gctctccgaa cgatcggacc aggtcgcgaa
     4321 ctggctgcgc gccctgggca tccgtcgcgg cgaccacctg atcgtgatgc tgaacaacac
     4381 gatcgagctg tgggagatca tgctcgccat caccaagatc ggcgcgatcg cgatccccac
     4441 ctcgaccctc ctgtcggcat ccgacctcgc ctaccgcatc gaccacggcc gcgcccgcgc
     4501 cgtggtcgcg ctgggcgggc tggcggatcg cgtcgaggat gccgacgtgc tccgcatcgc
     4561 ggtcggcgac gccaccgccg gctggcacga cttcgccgcg tccgccgatg ccgccgcggt
     4621 cttcgagccg gatggcccca ccccggcatc cgacaccgcc ctgctgtact tcaccagcgg
     4681 caccacgagc cggccgaagc tcgtgcagca cacgcacgtc tcctacccgg tcggccactt
     4741 gtcgaccatg tggtggctgg gcgtgcggcc cgacgacgtg catctgaaca tctcctcgcc
     4801 cggctgggcc aagcacgcct ggtcgagctt ctactcgccg ttcctcgccg aggcgaccgt
     4861 gttcgtctac aactacgacc gcttcgacgc gaacaccctc atgcaggtga tggacacgca
     4921 ccacgtgtcg accttctgcg caccgccgac cgtgtggcgg atgctgatcc aggccgacct
     4981 gtcgcgactg acgaacccgc cccgcgagct cgtcggagcc ggcgagcccc tgaaccccga
     5041 ggtcatcaac cgcgtgcgcg aggcgtgggg cagcacgatc cgcgacgggt acgggcagac
     5101 cgagatgacc gcgtgcgtgg gcaactcccc cggccagctc gtcaaggacg gctcgatggg
     5161 ccgcgcgctg cccggctatc cggtggtgct gctcgatccg gtcaccggcg aggtgacgga
     5221 tgccgagggc gagatcgcgc tcgacctgtc ggaggcgccg ctgggcctga tgtcgggata
     5281 ctacgaggat ccggagaaga ccgccgagtc ccgcgccggc ggcttccacc acaccggcga
     5341 catcgccgcg cgtgacgccg acgggtatct gacgtacgtc gggcgcgcgg acgacgtctt
     5401 caaggcatcc gattacaaga tctcgccgtt cgagctggag tccgcgctgc tcgagcacga
     5461 gctggtcgtc gaggcggcgg tcgtgccgag ccccgacccc acgcgcctgg cggtgccgaa
     5521 ggcgtacgtg atcctgcacg ggtccgcggg cagcggcgcg gagg
//
LOCUS       DATMSX010000446         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_167417,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000446 DATMSX010000000
VERSION     DATMSX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167417"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VN041_14295"
     CDS             <1..348
                     /locus_tag="VN041_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30250.1"
                     /translation="LLAARLRAGEVRRVDDRPLTRLVTELGDDHRLQEHSERMLAPLI
                     RHDEQHDGDLVRVLRAVVSHPGSRTAAAAASHLSRSVFYQRIALIADLLGADLDDGET
                     LSALHLALLASGR"
     gene            complement(364..720)
                     /locus_tag="VN041_14300"
     CDS             complement(364..720)
                     /locus_tag="VN041_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU30251.1"
                     /translation="MSALPLEVAGIVTDALSPTLPHEPLPADVVHDGTPGTGIRELGA
                     FRDVELGVWEMSEGAAVDTEADEVFIVLAGAARVDFIAPALPSIEVRTGSVVRLTEGM
                     RTLWTVHETLRKVYVA"
     gene            complement(717..2114)
                     /locus_tag="VN041_14305"
     CDS             complement(717..2114)
                     /locus_tag="VN041_14305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30252.1"
                     /translation="MGTTVFERFRPADAAITASLRDAKHAPFWLDDVERPSYPALTGA
                     AQADLVIVGGGYVGLWTAVRAKERDPQRSVILLEAARVGWAASGRNGGFCEASLTHGY
                     ENGLSRWPEEIEQLERLGLENLDGIEQTVQRYGMDVDWERTGQLAIATEPHQIEWLEG
                     DGFLDQDAVRSEVHSPTYLAGNWDREGCVMVHPAKLAIELARVAVELGVVIHEQSRVT
                     GISGNGRQTLTVAGGATVTADHVALATNVFPSLLKRNRLMTVPVYDYALMTEPLTTEQ
                     YDAIGWRNRQGLADSANQFHYYRLSADNRILFGGYDAIYHFGGKVRAEYEERPESYRR
                     LASHFFTTFPQLEGLTFTHRWAGAIDSCSRFCAFYGTARGGRVAYATGFTGLGVGASR
                     FGADVMLDKLAGMETERTRLKMVRTKPIPFPPEPAASIGVNLVRASMDRADHNEGRRD
                     LLLKTLDAVGMGFDS"
     gene            complement(2114..2581)
                     /locus_tag="VN041_14310"
     CDS             complement(2114..2581)
                     /locus_tag="VN041_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU30253.1"
                     /translation="MSDKTKSVALDDVSKRIIELLQQDGRRPYAEIGRAVGLSEAAVR
                     QRVQRLTDAGVMQIVAVTDPRQLGFARMAMIGIRTAGDPREVAEAVKAFDEIAYVVVT
                     LGTFDVLAEAICETDEDLLELVSNRVRAIPGVTHTESLIYAGLHKDFYNWGTR"
     gene            2681..4111
                     /locus_tag="VN041_14315"
     CDS             2681..4111
                     /locus_tag="VN041_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HWU30254.1"
                     /translation="MLTTLQNHIGGSFRDAHGTGTLDLIDPATGEVDGRIPLSDADDL
                     DAAYAAAAAAFPIWRDTTPAERQLALFRIADAMAARAEEFADLESQDTGKPRATLVTD
                     EIEQSVDQLRFFAGAARSLEGRAAGEYLAGHTSYVRREPIGVIGQVTPWNYPLNMAVW
                     KIAPALAAGNTVVLKPAESTPRSTLLLAEIVAEHTPAGTLNVVLGARETGATLVAHPV
                     PQMVAITGSVRAGMAVASSAANDLKRVHLELGGKAPAVVFADAPLQKAAEGIVAAAFF
                     NAGQDCTAAARVLVHSSVHDELVRMMVDRTRTHARTGGPHEEGVFFGPLSSPAQLAQV
                     QGFVDRLPAHARIETGGRRQGERGYFFEPTIVSGMRQDDEAVQDEVFGPLLTVQSFET
                     DDEALAMANGVRYALAASVWTRDHGRALRFTRDLDFGCVWVNTHIPFVSDMPHGGFKH
                     SGYGKDLSQYGFDDYTRIKHVMTALD"
     gene            complement(4125..4658)
                     /locus_tag="VN041_14320"
     CDS             complement(4125..4658)
                     /locus_tag="VN041_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU30255.1"
                     /translation="MVLELRPATVADADGIAEVHVRSWQEAYRGLMPQEVLDGLSVSE
                     RAANWARILGETGRASQTLVVLDGSRVVGWASFGDARDADADGAGSAVGTGELWGIYA
                     HPDAWSTGVGHLLISAVEDTLREAGHETAYLWVLAGNDRASGFYERHGWHADGASKVD
                     ERPGLVLNELRHVKRLA"
BASE COUNT          699 a         1707 c         1637 g          712 t
ORIGIN      
        1 ctgctcgcgg ctcgcctgcg cgcgggggag gtgcgccggg tcgacgaccg tccgctgacc
       61 cggttggtca ccgagctcgg cgatgaccac cgcctgcagg agcacagcga gcgcatgctg
      121 gccccgctca tccgccacga cgagcagcac gacggcgacc tggtgcgggt gctgcgcgcg
      181 gtcgtctcgc atcccggcag ccgcacggcc gcggcagcag cctctcacct ctcgcggtcg
      241 gtgttctacc agcgcatcgc gctgatcgct gacctgctgg gcgccgacct cgacgacggc
      301 gagaccctct cggccctgca cctggcgctg ctggcctccg gccgctgacc gaggccggcc
      361 acgtcaggcc acgtacacct tgcgcagggt ctcatgcacg gtccacaggg tgcgcatgcc
      421 ctcggtcagc cgcacgacgg accccgtccg cacctcgatc gagggcagcg ccggtgcgat
      481 gaagtcgacg cgcgcggcgc cggccagcac gatgaacacc tcgtcggcct cggtgtcgac
      541 ggcggcgccc tcactcatct cccagacacc gagctccaca tcgcggaagg ccccgagctc
      601 gcggatgccg gtgccggggg tgccgtcgtg caccacgtcc gcaggcagcg gctcgtgcgg
      661 aagcgtcggg gacagggcat ccgtcacgat gcccgcgacc tccagcggaa gcgcgctcac
      721 gagtcgaagc ccatgccgac ggcgtcgagc gtcttcagca gcaggtcgcg cctgccctcg
      781 ttgtggtcgg cgcgatccat cgaggcgcgg acgagattga cgccgatcga cgcggccggc
      841 tcgggcggga acgggatggg cttggtgcgc accatcttca gccgtgtgcg ctcggtctcc
      901 atcccggcca gcttgtcgag catcacgtcg gcgccgaacc ggctggcgcc gacgccgaga
      961 ccggtgaatc ccgtcgcata ggcgacgcgc ccgccgcggg cggtgccgta gaaggcgcag
     1021 aaccggctgc acgagtcgat cgcgccggcc cagcggtgcg tgaacgtcag cccctccagc
     1081 tgggggaaag tcgtgaagaa gtgcgaggcg agccggcggt acgactcggg ccgctcctcg
     1141 tactcggcgc gcaccttgcc gccgaagtga tagatggcgt cgtagccgcc gaacaggatg
     1201 cggttgtcgg cgctgagccg gtagtagtgg aactggttgg cgctgtcggc caggccctgc
     1261 cggttcctcc agccgatcgc gtcgtactgc tcggtggtga gcggctcggt catcagcgcg
     1321 tagtcgtaca ccggcacggt catcagccgg ttgcgcttga gcagcgaggg gaagacgttg
     1381 gtggcgagcg ccacatggtc ggcggtcacc gtcgcgccgc cggcgacggt cagggtctga
     1441 cggccgttgc ccgagatgcc ggtcacccgg ctctgctcgt ggatcacgac gcccagctcg
     1501 acggccaccc gggccagttc gatcgcgagc ttcgccggat gcaccatgac gcaaccttca
     1561 cgatcccagt tgccggcgag gtaggtgggg gagtgcacct ccgagcgcac ggcatcctga
     1621 tcgaggaagc cgtcgccctc cagccactcg atctggtgcg gttcggtcgc gatcgccaac
     1681 tggccggttc gctcccagtc cacatccatg ccgtagcgct gcacggtctg ctcgatgccg
     1741 tcgaggttct ccaggcccag gcgctcgagc tgctcgatct cctcgggcca gcggctcagg
     1801 ccgttctcgt agccgtgcgt caggctcgcc tcgcagaacc cgccgttgcg cccggacgcc
     1861 gcccagccga cgcgcgcggc ctcgagaagg atgaccgagc gctgcggatc gcgttccttg
     1921 gcacgcacgg cggtccacag accgacgtac ccgcctccga cgatgaccag gtcggcctgc
     1981 gcggcgcccg tgagcgccgg gtaggaggga cgctcgacgt cgtcgagcca gaacggcgcg
     2041 tgcttcgcgt cccgcagcga tgcggtgatc gcggcatccg cgggtcggaa ccgttcgaag
     2101 acggtggtgc ccatcagcgc gtgccccagt tgtagaagtc cttatggagg cccgcgtaga
     2161 tcagactctc ggtgtgcgtg acgcccggga tggcgcgcac gcggttgctg acgagctcga
     2221 gcaggtcctc gtcggtctcg cagatcgcct cggcgagcac gtcgaaggtg cccagcgtca
     2281 cgaccacgta ggcgatctcg tcgaacgcct tcaccgcctc ggcgacctcg cgcggatccc
     2341 cagcggtgcg gatgccgatc atcgccatcc gtgcgaagcc gagctgacgg ggatcggtga
     2401 ccgctacgat ctgcatgact ccggcatccg tcagccgctg cacgcgctgg cggacggccg
     2461 cctcgctgag accgaccgca cggccgatct cggcgtaggg gcgccggccg tcctgctgca
     2521 gcagttcgat gatccgcttg gagacgtcgt ccagcgccac gctcttcgtc ttgtcgctca
     2581 tgtgctgatt ctgacactat ctggatgccg aatcaaccga atccattgtt tggagagaag
     2641 ttttcttctg attccgcatt cgcatgggta gagtgtcggc atgctgacga cgctgcagaa
     2701 ccacatcggt ggctccttcc gggatgccca cggcaccggc accctcgacc tcatcgatcc
     2761 cgcgaccggc gaggtcgacg gccgcatccc cctctccgac gccgacgacc tggacgccgc
     2821 ctacgcggcc gccgctgcgg cgttcccgat ctggcgcgac acgacgccgg ccgagcgcca
     2881 gctcgcgctg ttccggatcg cggatgccat ggcagcgcgg gccgaggagt tcgccgacct
     2941 ggagtcgcag gacaccggaa aaccgcgcgc cacactcgtt accgacgaga tcgagcagtc
     3001 cgtcgaccag ctgcgcttct tcgccggggc cgcccgcagt ctggaggggc gcgccgccgg
     3061 ggagtacctc gccggacaca cctcctacgt gcggcgcgag ccgatcgggg tgatcgggca
     3121 ggtcacgccc tggaactacc cgctgaacat ggcggtgtgg aagatcgccc cggccctcgc
     3181 cgcaggcaac acggtcgtgc tcaagcccgc cgagtccact ccccgctcca ccctgctgct
     3241 cgccgagatc gtcgccgagc acaccccggc cggcacgctg aacgtcgtgc tcggggcgcg
     3301 ggagaccggg gcgacgctgg tcgcgcatcc ggtgccgcag atggtggcga tcaccggatc
     3361 ggtgcgcgcg ggcatggcgg tcgcctcatc cgccgcgaac gatctgaagc gcgtgcacct
     3421 ggagctgggc ggcaaggctc ccgccgtggt cttcgccgac gccccgctgc agaaggcagc
     3481 cgagggcatc gtggcggcgg cgttcttcaa tgccgggcag gactgcacgg ccgcggcccg
     3541 cgtgctcgtg cactcctcgg tccacgacga gctggtgcgg atgatggtgg accgcacgcg
     3601 cacgcacgcc cgcaccggcg ggccgcacga ggagggtgtg ttcttcgggc cgctctccag
     3661 ccccgcgcag ctcgcgcagg tgcagggctt cgtcgatcgc ctccccgccc acgcgaggat
     3721 cgagaccggc ggccgccggc agggcgagcg cgggtacttc ttcgagccga ccatcgtctc
     3781 gggtatgcgg caggacgacg aggccgtgca ggacgaggtg ttcggaccgc tgctcaccgt
     3841 gcagtccttc gagaccgacg acgaggcgct cgcgatggcg aacggcgtgc ggtacgcgct
     3901 ggcggcatcc gtgtggaccc gcgatcacgg ccgcgccctg cggttcaccc gcgacctgga
     3961 cttcggatgc gtgtgggtga acacccacat ccccttcgtg tccgacatgc cgcacggcgg
     4021 cttcaagcac tccggctacg gcaaggacct gtcgcagtac ggcttcgacg actacacgcg
     4081 catcaagcac gtgatgaccg cactggactg agcgggcggg tgcctcaggc caggcgcttc
     4141 acgtggcgga gctcgttgag cacgaggccg gggcgctcgt cgaccttgct cgcgccgtcg
     4201 gcgtgccagc cgtggcgctc gtagaagccg gatgcccggt cgttgcccgc gagcacccac
     4261 aggtacgcgg tctcatgacc ggcctcccgc agagtgtcct cgacggcgga gatcagcaga
     4321 tgcccgacgc cggtggacca ggcatcgggg tgcgcgtaga tgccccacag ctcgccggtg
     4381 ccgaccgctg agccggcacc atcggcatcc gcatccctgg cgtcgccgaa gctcgcccac
     4441 cccacgaccc gggagccgtc gagcaccacg agcgtctgcg atgcgcgccc ggtctcgccc
     4501 aggatgcgcg cccagttcgc ggctcgctca gagacggaca ggccgtcgag aacctcctgc
     4561 ggcatcaacc cgcgataggc ctcctgccag gagcgcacgt gcacctcggc gatgccgtcg
     4621 gcgtccgcga cggtcgcggg gcgcagctcg aggaccatcg gagctcagcc cgcgttgcgc
     4681 cgggcctccc agcccgagcg caccatctcg tcgaccgtgt accgcatggt ccagtccagg
     4741 tcgcgggcgg cgagg
//
LOCUS       DATMSX010000447         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_177292,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000447 DATMSX010000000
VERSION     DATMSX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177292"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1195
                     /locus_tag="VN041_14325"
     CDS             143..1195
                     /locus_tag="VN041_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWU30256.1"
                     /translation="MSLLELESVRRFYKQKHTMFSRGAHGQVRAVDGVDFTVEEGETV
                     GLVGESGCGKSTLARIICGLEAPSEGTVRWNGADVSAMSHSERKTLLHRQVQMVFQDP
                     HASLDPRRSVGQSIAEGITTHRLLPASKVEARVGELLTQVGLNPDHAHRYPHEFSGGQ
                     LQRIGIARALAVEPKLLVCDEPVSALDISVRAQVLNLLNRLKRELGLSIIFIAHDLDV
                     VQHMSDRIVTMYLGKVVEQGECDDVYGATAHPYTRALLSAIPRKLAPNERLERVVLEG
                     DPPSPIDPPPGCPFHTRCYAAIERCATDVPALDAVPGGRPEHRASCHLAAQITLSRVG
                     MVSTISSSPSPSERNT"
     gene            1195..2784
                     /locus_tag="VN041_14330"
     CDS             1195..2784
                     /locus_tag="VN041_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30257.1"
                     /translation="MKSTTSRIAVGIVAAAACATMLTGCLGQTADAGDGKTSFVAVNN
                     GEIGRITGAQNPGSQIAMALCEPLTGIEEDGKVFMRGAESLESEDQVHWTVKVADSHT
                     FSDGTPITAKTYVDTFNFVGAGKNGMPSNYAYGPIKGYDELNPAEGEATATEFSGIKL
                     EDEHTFTIEMKTPNNDVPYILSQLAFCPMPESAFADPVAYDKKPIGNGVYTLEKLDPQ
                     VEAVLKKDPKYKGWVPKGAADQITFRVYTDTNTAYQDVVAGNADVLRSLPPGLIAQGR
                     NSLGEKGLTAIDRNTLETYFTWPTYLDKTYPLEVRQAFSMIVDRESISKDLFKDATVP
                     AGSLMPNSVSAYREDACGQYCTFDPAAAKALIAKSGFTGSIPLNYSSENTTDAATALA
                     VSNSAKEIGLDVEPKPVPAAALSETINSYKLDGPVIQLWGSSFPAASEWIASVYVDAN
                     YRLQYTDPEGQAAVDGALAAATPEESDALWQKAEDRILADQVIQPLYQQVMYIAHTEC
                     VTPHAAGGDMQIYRTQVTCGK"
     gene            complement(2849..>3510)
                     /locus_tag="VN041_14335"
     CDS             complement(2849..>3510)
                     /locus_tag="VN041_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWU30258.1"
                     /translation="RFEAAPSGVVTVSGFLSAAQALFGPLVRELGTGDDAPTIRFTDE
                     DVAQSEFPALTARYDLVLAHRMEHSAPWPRSGIRSVPLAIEPLDVALATSHPLAGRES
                     LTPEQVVGERWVTSRTGYSPDDVLRTISAVTNRPVEIVHRINDYGVAAAVISAGGVVG
                     MLPRYTSVAAGVTGVVRLPLDGVSTNRMIDVLARPENLHRRSVRLVAEALERVMATLA
                     A"
BASE COUNT          598 a         1229 c         1183 g          500 t
ORIGIN      
        1 atcgcgcagc gcccgcccgg ctgcagcttc aacccgcgct gcgggtacgc gacggccgag
       61 tgccgcaccg agctgccgct gctcgacgtt ttcgccccgg agcgggccgc ggcctgccac
      121 cacaagcagg aggtgatcgc cgatgtcgct cttggagctt gaatcggtgc gacgcttcta
      181 caagcagaag cacacgatgt tctcgcgcgg agcgcacggc caggtgcgcg cagtggacgg
      241 cgtcgacttc accgtcgagg agggtgagac cgtcggcctg gtgggggagt ccggctgcgg
      301 caagtccacg ctcgcacgca tcatctgcgg gctggaggcg cccagcgagg gcacggtgcg
      361 ctggaacggc gccgacgtct cggccatgtc gcactccgag cgcaagacgc tgctgcaccg
      421 tcaggtgcag atggtcttcc aggatccgca cgcctcactc gacccgcgcc gctcggtcgg
      481 tcagtcgatc gcagagggga tcaccacgca ccggctgctg ccggcgtcga aggtggaggc
      541 gcgcgtgggg gagctgctca cgcaggtggg cctgaacccc gaccacgcgc accgctatcc
      601 gcacgagttc tccggcgggc agctccagcg catcggcatc gccagggcac tggcggtcga
      661 gccgaagctg ctggtctgcg acgagccggt ctcggcgctg gacatctccg tgcgcgcgca
      721 ggtgctcaat ctgctgaacc ggctgaagcg cgaactcggt ctgtcgatca tcttcatcgc
      781 gcacgacctc gacgtcgtgc agcacatgtc cgaccgcatc gtcacgatgt acctgggcaa
      841 ggtcgtcgag cagggcgagt gcgacgacgt gtacggcgcc accgcgcatc cctacacccg
      901 agcgctcctg tcggccattc cccgcaagct ggcgccgaac gagcgcctgg agcgcgtcgt
      961 gctcgagggt gacccgccct cgccgatcga ccctccgccc ggatgcccgt tccacacgcg
     1021 ctgctacgcg gcgatcgaac ggtgcgccac ggacgtgccc gctctcgacg cggtcccggg
     1081 cggacggccg gagcaccggg catcctgcca tctcgccgct cagatcaccc tatcccgcgt
     1141 cggcatggtc tccacgatct cttcctcacc ctcaccatcc gaaaggaaca cctgatgaaa
     1201 agcaccacca gccgcatcgc ggtcggcatc gtcgccgccg cagcgtgcgc gacgatgctg
     1261 accggatgtc tcggtcagac cgccgacgcc ggcgatggca agacgtcctt cgtcgccgtc
     1321 aacaacggcg agatcggccg catcaccgga gcgcagaacc ccggaagcca gatcgccatg
     1381 gcgctctgcg agccgctgac gggcatcgag gaggacggca aggtcttcat gcgcggtgcc
     1441 gagtcgctcg agagcgagga ccaggtgcac tggaccgtga aggtcgcgga cagccacacc
     1501 ttctcggatg gcacgccgat caccgcgaag acctatgtcg acaccttcaa cttcgtcggc
     1561 gcaggcaaga acgggatgcc ctcgaactac gcgtacggcc cgatcaaggg ctacgacgag
     1621 ctgaaccccg ccgagggcga ggccacggcc acggagttct cgggcatcaa gctcgaggac
     1681 gagcacacgt tcacgatcga gatgaagacg ccgaacaacg acgtgccgta catcctctcg
     1741 cagctggcgt tctgtccgat gccggagagc gcgttcgcgg atccggtggc ctacgacaag
     1801 aagccgatcg gcaacggcgt gtacacgctg gagaagctgg acccgcaggt cgaggccgtg
     1861 ctgaagaagg atccgaagta caagggctgg gttccgaagg gcgccgcgga ccagatcacg
     1921 ttccgcgtct acaccgacac gaacaccgcg taccaggacg tggtcgcggg caacgccgac
     1981 gtgctgcgca gcctgccgcc ggggctcatc gcccagggca ggaactccct cggcgagaag
     2041 ggcctgaccg cgatcgaccg caacacgctg gagacgtact tcacctggcc gacctacctc
     2101 gacaagacct atccgctcga ggtgcgccag gcgttctcga tgatcgtcga ccgcgagagc
     2161 atctcgaagg acctgttcaa ggatgccacc gtgccggccg gttcgctgat gccgaactcc
     2221 gtctccgcat accgtgagga tgcgtgcggg cagtactgca cgttcgaccc tgctgccgcg
     2281 aaggcgctca tcgcgaagtc gggcttcacc ggcagcattc ccctgaacta ctcgtccgag
     2341 aacacgacgg atgccgcgac cgcactcgcg gtctcgaact ccgcgaagga gatcgggctc
     2401 gatgtcgagc cgaagccggt gcctgccgcc gcgctctcgg agacgatcaa ctcctacaag
     2461 ctggacggcc ccgtcatcca gctctggggc tcgtccttcc cggccgcgtc ggagtggatc
     2521 gcctcggtgt acgtcgacgc gaactaccgc ctgcagtaca ccgacccgga gggccaggcc
     2581 gctgtggacg gcgcactcgc cgcggcgacg ccggaggagt ccgacgccct ctggcagaag
     2641 gccgaggacc gcatcctcgc ggaccaggtg atccagcccc tgtaccagca ggtgatgtac
     2701 atcgcccaca ccgagtgcgt gaccccgcac gcggccggcg gtgacatgca gatctaccgc
     2761 acgcaggtca cctgcggcaa gtgacccgct gacggcggat ccggcgaggg ccgggacgga
     2821 actcctccgt cgcggccctc acgccggttc aggcggccag cgtcgccatc acccgttcga
     2881 gcgcctcggc gaccaggcgc accgaccgcc ggtggaggtt ctcggggcgg gccagcacgt
     2941 cgatcatgcg gttggtgctg acgccgtcga gcggcaggcg caccacgccg gtgacgcccg
     3001 ccgcgaccga ggtgtagcgc ggcagcatcc cgaccacgcc gcccgcggag atcaccgcag
     3061 ccgcgacgcc gtagtcgttg atgcggtgca cgatctcgac gggccggttg gtgacggccg
     3121 agatggtgcg cagcacgtcg tcgggggagt agccggtgcg actggtgacc cagcgctcgc
     3181 cgaccacctg ctcgggcgtc agcgactccc tgccggccag aggatgcgat gtggcgagcg
     3241 cgacgtcgag cggttcgatc gccagcggca cggagcggat gccgctgcgc ggccacggtg
     3301 cgctgtgctc catgcggtgg gcgagcacca ggtcgtagcg cgcggtgagt gcggggaact
     3361 cgctctgtgc gacatcctcg tcggtgaacc ggatggtggg ggcgtcgtcg ccggtgccga
     3421 gctcgcgcac gagcgggccg aacagggcct gcgccgcgct gaggaacccg ctcaccgtga
     3481 ccactcccga cggcgccgcc tcgaagcggt
//
LOCUS       DATMSX010000448         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_54148,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000448 DATMSX010000000
VERSION     DATMSX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54148"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VN041_14340"
     CDS             complement(<1..863)
                     /locus_tag="VN041_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWU30259.1"
                     /translation="MSQIDPGTSAWLRTRNDREALHLFLEHGALTRTRLGELSGMSKP
                     TATQMISRLESAGLIGPVGEVAGSRGPSAVSWGVRSDRVAGVAVSMLEDCIQAVVVDA
                     LDRRHPIVELEVRGEQRSPERDIDRAIDAACEASGIERSTVEVVTAGVQAAVSSESDA
                     LSLTDSLPGWPSVGARARIESELGIAVVLENDVNLATMAERAFGVAQHAGSFALLWLG
                     VGLGVGVDLGGRIHRGAHGGAGEIGYLVVAGRSQHSGAVTTQLLGSDSILALVDAPAG
                     ARYPDGIAALAG"
     gene            complement(950..1807)
                     /locus_tag="VN041_14345"
     CDS             complement(950..1807)
                     /locus_tag="VN041_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30260.1"
                     /translation="MRTTDARTQPPTLEARHLVKDFHLRSGMRTNVLHAVKDVSFTIE
                     AGRTVALVGESGSGKSTIARMLMKLENPTSGEILLDGRPTGRGGRARAAYRSEVQMVF
                     QDPFASLNPFHTIMHHLERPLRIHHPGLSGARVRERALELLERVRLTPAESFIERRPH
                     ELSGGQRQRVAIARALAPGAQFIVADEPVSMLDVSIRLGVLNLLADLQREDHLGVLYI
                     THDLATARHFSDEIMVLYKGDVVERGPADEVILNPKHEYTRTLLAAAPEPENLGRLRD
                     EVRAELAAG"
     gene            complement(1804..2736)
                     /locus_tag="VN041_14350"
     CDS             complement(1804..2736)
                     /locus_tag="VN041_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30261.1"
                     /translation="MTDLIISPAAEPGAVPVDTEPVLTARGVTIEYEVDPPVQAVREV
                     SLTLNRGEILGLAGESGCGKTTLAYGLNRLLKAPALMTSGEIVFHDRSGADIDVAALG
                     GEALRAFRWDKISMVFQGAMNSLNPVITVRAQIFDIFETHRPGMSRRDKQTRAEELLT
                     LVGVDPARLASFPHELSGGMRQRMMIAMALALDPQVMIMDEPTTALDVVVQRGIIREI
                     MRLREKLGFAVIFITHDLPMLIEISDRIAVMLDGRIVEQGPAEQIYRAPEHEYTRRLL
                     SSFPSLRGQRGGFVRAASGVVGAEAAGPTGEGSR"
     gene            complement(2733..3797)
                     /locus_tag="VN041_14355"
     CDS             complement(2733..3797)
                     /locus_tag="VN041_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30262.1"
                     /translation="MAELIEAPMGADADTGAVTPTSARTHTGAYDAPADTHTVAVARM
                     GRSGRALPRITGKLLSGLIIIGAIALFGLLGPLVVGDPHDASHEPLLPPSPAHLLGTT
                     KLGHDVLAQIATGTQGSLFVGVVAGLVALLLALVFGVLAGYFGGALDEVLMLITNIML
                     VIPGLPLVMIIATYVQHTEGLGALARSSLLVALVLGITGWAGSAVVLRGTARSLRTRD
                     YVAASVVAGERPLRIIFVEILPNLVPLLAAQLIFGVIFAVLGEAGLSYLGLGPTGSIT
                     LGTVLNDAQTGQAVGSGAWWWFVPPGAIIALIGTGLSLINFAIDEIVNPKLRLAPAAA
                     RRQRRAQRAEAAARREKVAA"
     gene            complement(3790..4794)
                     /locus_tag="VN041_14360"
     CDS             complement(3790..4794)
                     /locus_tag="VN041_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30263.1"
                     /translation="MRFWTRRVVFYVITLWAAVSLNFLLPRMMPGDPAAIMIGKLRRA
                     SGGRPLSEATITSIHALLGTGKDTSLWAQYLVYWGRMLQGDLGVSSTRYPASVGDLIA
                     DALPWTVTLVGTATVISFVIGIVAGAWVGWRRGTWVDQLVPVTTFLQSVPYFWLALVL
                     VAVFSVQLGLLPIFGGYGVFEYPAGPEWSLGFIGSVVVHALLPATTIVISSVGGWLLG
                     MRNMMVQEMAEDYVLTAEAKGLRPSRIRRAYAARNAAIPSLAGFGIALGFVVAGSIVT
                     EQVFSYPGIGKLMIQAVQGLDYALMQGVFLVITLTVLAANVLVDLLYGFIDPRVRRNG
                     "
     gene            complement(4833..6362)
                     /locus_tag="VN041_14365"
     CDS             complement(4833..6362)
                     /locus_tag="VN041_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30264.1"
                     /translation="MTPIANNFNPFAVDTAAHLTFGAIYEPLFFFNQLSAEAPVGLIG
                     DSFEFNADGTVLTIRIKPDQKWSDDADLTAEDVAFSLGYGSNRDPDTVSVEATDATTV
                     VVTYSTPKFTAASMILGATFIVPEHIWSKITDYMSETNPKPVGSGPYVLKTFTDAAYT
                     LEANPLFREGAPDIKKVQDVGIDSNQSAEDLLKTGELDWVGQFVANPDGVTGGGRVAT
                     MNQQQDPTVIMTCADAALGCTGAQTDPAVRQALNAAMDRASISKKAFAGLAGESSPSF
                     ALLPRDEKWLSDPSLATSPQHADAAEAGRILEAAGYTKDSDGFYGKDGTAIELDLFSP
                     DGWTDYNDAAKLIAAQAAKAGIRVNARTVSEAEYWTPVSNGDFQLALYGLTQSLVADP
                     FSNYDQYFATGATAKVGEMPAKGQNYARYSNADVDAAVAAAGATQDEGVKKEAYARIQ
                     AQIAQDLPYIPVVLNASQAFYNTAEFAGWPTADDLYANPLPYLSTASAVILTHLHPVE
                     K"
     gene            complement(6647..>7338)
                     /locus_tag="VN041_14370"
     CDS             complement(6647..>7338)
                     /locus_tag="VN041_14370"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HWU30265.1"
                     /translation="FARAGQIAGWRGRWLGQVGLGAGSKKDASARGVHYRDVDGVVRG
                     AIAYTLKELPGSFRNSMTIRQLVAETDEALSALWGFAVNHDLVDRIEADLRPVDDPVV
                     RLVANPHAVDVAVRDHGWLRILDVPAALRARRYAASVDTAIRVEDGYGFAEGTWRLSI
                     DAAGAAEVEATDAAPDVVLDVATLSSAYAGGVPLTQLAAAGRLQGDAVAVMALGRALH
                     ADPAPTLSIWY"
BASE COUNT         1125 a         2573 c         2494 g         1146 t
ORIGIN      
        1 cccgcgaggg ccgcgatgcc gtccgggtac cgagcgcccg cgggcgcgtc cacgagcgcg
       61 aggatgctgt ccgatccgag cagctgcgtg gtcaccgcgc cactgtgctg ggagcgtccg
      121 gccacgacga ggtagccgat ctcaccggcc ccgccgtggg ctccgcgatg gatccggccg
      181 cccagatcga cgcccacgcc cagcccgacg cccagccaca gcagggcgaa gctgcctgcg
      241 tgctgcgcga cgccgaaggc ccgctccgcc atggtggcga gattgacgtc gttctccaga
      301 acgacggcga tgccgagttc ggactcgatt cgcgcacggg ccccgacgga gggccatccg
      361 ggcagggagt cggtcaggga gagggcgtcg gactcgctgc tcaccgcggc ctggacgcct
      421 gcggtcacca cctccacggt gctcctctcg atgccggacg cctcgcacgc cgcgtcgatc
      481 gcccggtcga tgtcgcgctc cggcgaccgc tgctcgccgc gcacctcgag ttccacgatc
      541 ggatgcctgc ggtccagcgc gtcgacgacc accgcctgga tgcagtcctc gagcatgctc
      601 accgccacgc ctgcgacgcg gtcgctccgc acaccccagc tcacggcgct ggggccacgg
      661 ctgcctgcga cctcgcctac tggaccgatc agccccgcgc tctccagccg cgagatcatc
      721 tgcgtcgcgg tgggtttcga catcccggag agctcgccga gtcgcgtgcg agtgagcgcg
      781 ccgtgctcga ggaacaggtg gagcgcctcg cggtcgttcc gcgtgcgcag ccacgcggac
      841 gtgcccgggt cgatctgcga catgagtgct cctctcgggg gttcgtcgtc gtgcggggac
      901 accgcacgac gaacggtcgc ctgcggcggg aacggaatcg attcggccgt cagcccgcgg
      961 cgagttccgc gcggacctcg tcgcgcagcc ggccgaggtt ctcgggttcg ggcgcggcgg
     1021 cgaggagcgt cctggtgtac tcgtgcttcg gattgaggat gacctcgtcc gccgggccgc
     1081 gctctacgac gtcgcccttg tagagcacca tgatctcgtc gctgaaatgg cgagccgtgg
     1141 cgaggtcgtg ggtgatgtac agcacgccca gatggtcctc gcgctgcagg tcggccagca
     1201 ggttcagcac gccgagcctg atcgacacgt cgagcatcga caccggctcg tccgccacga
     1261 tgaactgtgc gcccggggcg agcgcgcggg cgatcgcgac ccgctgccgt tggcctccgg
     1321 agagctcgtg cggcctgcgc tcgatgaacg actccgcggg agtcagccgc acccgctcca
     1381 gcagctcgag cgctcgctcg cgcacgcgcg ccccggacag ccctggatga tggatccgca
     1441 gcgggcgctc caggtggtgc atgatcgtgt ggaacgggtt cagcgacgcg aacgggtcct
     1501 ggaacaccat ctgcacctcg gagcggtacg cggccctggc ccgcccgccg cgaccggtcg
     1561 gccgcccgtc gagcaggatc tccccgctgg tggggttctc cagcttcatc agcatccgcg
     1621 cgatcgtcga cttcccgctg ccggactcgc cgaccagcgc gacggtcctc cccgcttcga
     1681 tggtgaacga gacgtccttc acggcgtgca gcacgttcgt ccgcattccc gagcgcaggt
     1741 ggaagtcctt gaccaggtgt ctggcctcca gcgtcggcgg ctgggtcctg gcgtccgtcg
     1801 tcctcatcgc gatccctctc cggtcggtcc ggccgcttcc gcgccgacga cgcctgacgc
     1861 ggcgcggacg aatccgcccc gctgccccct caggctcggg aagctcgaca gaagccgccg
     1921 ggtgtactcg tgctcgggtg cacggtagat ctgctcggcg ggaccctgct cgacgatccg
     1981 tccgtccagc atcaccgcga tccgatcgct gatctcgatc agcatcggaa gatcgtgggt
     2041 gatgaagatc acggcgaagc cgagcttctc ccgcagccgc atgatctcgc ggatgatccc
     2101 ccgctgcacc acgacgtcca gcgctgtggt cggctcgtcc atgatcatca cctgcggatc
     2161 cagggcgagc gccatcgcga tcatcatgcg ctggcgcatg ccgccggaca gctcatgcgg
     2221 gaagctggcg agccgagcgg gatcgacgcc gaccagcgtc agcagctcct cggcccgcgt
     2281 ctgcttgtcc cgccgcgaca tgccgggccg atgcgtttcg aagatgtcga agatctgcgc
     2341 gcggaccgtg atcaccgggt tgagggagtt catcgcaccc tggaagacca tcgagatctt
     2401 gtcccagcgg aacgcccgga gcgcttcgcc gccgagggcg gcgacgtcga tgtccgcacc
     2461 ggatcgatcg tggaagacga tctcgcccga ggtcatcagc gccggcgcct tcagcagccg
     2521 gttgagcccg tacgcgagcg tcgtcttgcc gcatccggac tctcccgcga gacccaggat
     2581 ctcaccgcgg ttcagcgtca gcgagacctc gcggaccgcc tgcacgggcg gatccacctc
     2641 gtattcgatc gtcacgccgc gcgccgtgag aacgggttcc gtgtccacag gtacggcccc
     2701 cggctccgcg gcgggcgaga tgatgaggtc ggtcatgccg ccaccttctc tcgcctggcg
     2761 gctgcttcgg cgcgctgggc gcggcgctgc ctgcgggcgg ccgccggggc gagccgcagc
     2821 ttcgggttca cgatctcgtc gatcgcgaag ttgatcagcg agagccccgt ccctatcagg
     2881 gcgatgatcg cgcccggtgg cacgaaccac caccaggcac cgctgcctac cgcctgtccg
     2941 gtctgcgcat cgttcagcac ggtgcccagc gtgatcgatc cggtcgggcc gaggcccaga
     3001 taggacagac ctgcctcgcc gagcacggcg aagatcacgc cgaagatcag ctgagccgcc
     3061 agcagcggaa cgagattggg caggatctcc acgaagatga tgcgcagagg acgctcccct
     3121 gcgacgacgg atgccgcgac gtagtcccgc gtgcgcaggg acctcgcggt cccgcgcagc
     3181 accacggccg atcccgccca tccggtgatg ccgagcacca gtgccaccag cagcgagctg
     3241 cgggccagcg ctccgagccc ttcggtgtgc tgcacgtagg tggcgatgat catcaccaga
     3301 ggcagccccg ggatgacgag catgatgttc gtgatcagca tcagcacctc gtccagtgca
     3361 ccaccgaagt atccggccag cacgccgaac accagtgcga gcagcagggc cacgagtccg
     3421 gcgaccaccc cgacgaagag cgacccctgg gtccccgtcg cgatctgcgc cagcacgtcg
     3481 tgaccgagct tggtggtgcc gagcaggtgc gcgggagacg gcgggagcag cggttcgtgg
     3541 gacgcatcgt gcggatcccc cacgaccagt ggacccagca ggccgaacag cgcgatcgcg
     3601 ccgatgatga tcagaccgct cagcagcttg ccggtgatcc tcggcagagc gcgcccggag
     3661 cggcccatcc gcgcgaccgc gacggtgtgc gtgtccgcgg gcgcgtcgta cgcgcccgtg
     3721 tgagtgcgtg cgctcgtggg cgtgactgcg cccgtgtccg cgtcggcgcc catgggcgcc
     3781 tcgatcagtt cagccatttc tccgcaccct tggatcgatg aacccgtaca gcaggtcgac
     3841 gagcacattg gcggcgagca ccgtgagggt gatcaccagg aacacgccct gcatgagcgc
     3901 gtagtccagt ccctgcacgg cctggatcat cagcttgccg atgccggggt agctgaacac
     3961 ctgctcggtg acgatggacc ccgccacgac gaacccgagc gcgatgccga atcccgccag
     4021 gctcgggatg gccgcgttcc gcgcggcgta ggcgcggcgg atccgagacg gccgcagccc
     4081 cttcgcctcg gcggtgagga cgtagtcctc ggccatctcc tgcaccatca tgttgcgcat
     4141 gcccagcagc catccgccca ctgaggagat cacgatcgtc gtcgccggca gcagcgcatg
     4201 cacgacgacg ctgccgatga agccgaggct ccactccgga cccgccggat actcgaacac
     4261 gccatagccg ccgaagatcg gcagcaggcc gagctgcacc gagaagacgg cgaccagcac
     4321 cagggcgagc cagaagtacg ggaccgactg caggaacgtc gtcacgggga cgagctggtc
     4381 gacccaggtc ccccgtcgcc atccgaccca ggctccggcg acgatcccga tgacgaagga
     4441 gatcacggtc gccgtcccca cgagggtcac ggtccacggc agcgcgtcgg cgatgaggtc
     4501 acccaccgac gcgggatacc gcgtcgagga gactccgagg tccccctgca gcatccgccc
     4561 ccagtagacg aggtactgag cccacaggct cgtgtccttc ccggtgccca gcagcgcgtg
     4621 gatcgacgtg atcgtggcct ccgagagcgg gcgccctccg ctggcgcggc gcagcttgcc
     4681 gatcatgatc gccgcggggt cgcccggcat catcctcggc aggaggaagt tgagcgagac
     4741 ggcggcccag agcgtgatca cgtagaagac gactcgacgc gtccagaacc tcatgcgctc
     4801 ctcctcagga cccgaaccat gtgcagaggc ccctacttct cgaccgggtg cagatgcgtc
     4861 aggatgacgg cggaggccgt ggacaggtac ggcagcgggt tcgcgtacag gtcgtcggcg
     4921 gtgggccagc cggcgaactc ggcggtgttg tagaacgcct gcgaggcgtt gagcaccacc
     4981 gggatgtacg gcagatcctg cgcgatctgc gcctggatcc tcgcgtacgc ctccttcttg
     5041 acgccttcgt cctgggtcgc accggccgcg gcgacggccg cgtcgacatc ggcgttgctg
     5101 tagcgcgcgt agttctgccc cttggccggc atctcgccga ccttcgccgt ggcccccgtg
     5161 gcgaagtact ggtcgtagtt cgagaaggga tccgcgacca acgactgcgt caggccgtac
     5221 agcgcgagct ggaagtcgcc gttggagacc ggcgtccagt actcggcctc ggagacggtt
     5281 cgcgcgttga ccctgatccc ggccttcgcc gcctgcgcgg cgatgagctt cgccgcgtcg
     5341 ttgtagtcgg tccagccgtc cggcgagaac aggtcgagct cgatggcggt gccgtccttg
     5401 ccgtagaagc cgtcggagtc cttcgtgtac ccggcggctt ccaggatccg gcccgcctcg
     5461 gcggcatcgg cgtgctgcgg gctggtggcg agcgacgggt cgctcagcca cttctcgtcc
     5521 cgcggcagca gggcgaagct cgggctggac tcccctgcca ggcccgcgaa cgccttcttg
     5581 ctgatcgatg cacggtccat cgccgcattg agcgcctggc ggacggcggg atcggtctgc
     5641 gcgcccgtgc agcccagcgc cgcgtcggcg caggtcatga tcacggtggg gtcctgctgc
     5701 tggttcatgg tggcgaccct gccgccgccc gtgacgccgt ccgggttggc cacgaactgc
     5761 ccgacccagt cgagctcgcc ggtcttcagc aggtcctcgg ctgactggtt ggagtcgatc
     5821 ccgacgtcct gcaccttctt gatgtcgggc gcaccctcgc ggaacagcgg gttggcctcg
     5881 agcgtgtagg cggcgtcggt gaaggtcttg aggacgtacg ggccggatcc gaccggcttc
     5941 gggttggtct ccgacatgta gtcggtgatc ttcgaccaga tgtgctcggg cacgatgaag
     6001 gtggccccga ggatcatcga ggcggcggtg aacttcggcg tcgagtaggt cacgaccacg
     6061 gtggtcgcgt ccgtggcctc gaccgagacg gtgtccggat cgcggttgga gccgtagccc
     6121 aggctgaacg ccacgtcctc ggcggtgagg tcggcgtcat cactccactt ctgatccggc
     6181 ttgatcctga tcgtgaggac cgtgccgtcc gcgttgaact cgaaggagtc gccgatgagt
     6241 cccaccggcg cctcggcgga gagctggttg aagaagaaca gcggctcgta gatcgcgccg
     6301 aaggtcaggt gcgccgccgt gtcgacggcg aaggggttga agttgttcgc gatcggcgtc
     6361 atcgaccccg accacacacg cagcggccgt tcgccggtcg actgggcgcc tgaaggcgcg
     6421 catcctccca atgccagggc gagtgcggca gtgcctgcga caagaggaat cgtcttcctc
     6481 caccggatgt cgtgcatggg gacctcgtct caagtgcttc gttggcttgt cgagcggtgc
     6541 ggagaggact ccgccagaag cgaggttata gtaaaggttc tttccggtaa agacctttcc
     6601 aattgatttc tgcagagaag ttcgcagggc ccccgtgcct gcgggctcag taccagatgc
     6661 tgagcgtggg tgccgggtcg gcgtgcaggg cgcgtccgag agccatgacg gccaccgcgt
     6721 cgccctgcag ccggccggcg gctgcgagct gggtgagcgg cactcccccg gcatacgcac
     6781 tggacagcgt cgcgacgtcg agcacgacgt cgggcgcggc atccgtcgcc tcgacctcgg
     6841 cagcacctgc cgcgtcgatg ctcagccgcc acgtcccctc cgcgaagccg tacccgtcct
     6901 cgacgcggat cgccgtgtcg acggatgccg catagcggcg cgcgcgcagg gccgcaggca
     6961 cgtccaggat gcgcagccag ccgtggtccc gcaccgcgac gtccaccgcg tgcggattcg
     7021 cgaccaggcg caccaccggg tcgtcgaccg gacgcaggtc ggcctcgatc cgatcgacga
     7081 ggtcgtgatt caccgcgaag ccccacagag cgctcagcgc ttcgtcggtc tccgcgacga
     7141 gctggcggat cgtcatgctg ttccggaacg atccggggag ctccttcagt gtgtaggcga
     7201 tcgcgccgcg cacgacgccg tcgacgtccc ggtagtgcac gccgcgcgcg ctggcatcct
     7261 tcttcgagcc cgcgcccagt cccacctgac cgagccagcg tccgcgccag cccgcgatct
     7321 ggccggcccg cgcgaagc
//
LOCUS       DATMSX010000449         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_120927,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000449 DATMSX010000000
VERSION     DATMSX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120927"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..827)
                     /gene="dapB"
                     /locus_tag="VN041_14375"
     CDS             complement(81..827)
                     /gene="dapB"
                     /locus_tag="VN041_14375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWU30266.1"
                     /translation="MTTQVALVGGSGKLGRIIHSVIDELDGFEVRNVLGSASDLSELD
                     GADLVVDASTPAVSIEVVRAALERGISILVGTSGWSQERMSLVRPLQASAGTGVVFIP
                     NFSLGSALGTALAAAAAPFFPFAEIVEAHRDSKIDSPSGTAVRTAEQMAEAREERGPF
                     DAPHVDQRARGQQVAGIPVHSLRRPGVIARQEAILSGAGESLTIVHDTVDSAAAYAPG
                     IRLAVPFARDNRDLVIGLENIIDLGLRPKA"
     gene            complement(881..1483)
                     /locus_tag="VN041_14380"
     CDS             complement(881..1483)
                     /locus_tag="VN041_14380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWU30267.1"
                     /translation="MTHYIYLVRHGEHQDAEHGIDDGPLSARGRRQAELIADRLSGLP
                     LDAVWHSPLLRATETARAIAERLPSVDPVPSALLFDCVPTGMTEDTPAAYESFFGSVS
                     EAEIEAGSAQMSDAVGEFLRRRNGEVHEVLITHNFVISWFVREVLGAPDWRWMTLNQA
                     HCGLSVIAQKQGRPWTLISHNDLGHLPMELRTGLPDVPSV"
     gene            complement(1564..2589)
                     /locus_tag="VN041_14385"
     CDS             complement(1564..2589)
                     /locus_tag="VN041_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU30268.1"
                     /translation="MRIFGVGTADQRSRPDEALDEHPSAPIPVVGPGVGEGIRVPLGE
                     TGFSIFPLMLGAAEFGWNVDLPTSHALLNRYVEFGGNAIHTADGFSGGRSEHIIGQWM
                     RSRADHDGLVLSVRIGAHADNPGLGSVNLVRAVEGSLTRLGVERIDVLYLDASLDETT
                     NLEDTLATTEWLRDAGKIASVGAFSFAPERLVEARILASAGYPRLDVLDEPYNLVRRT
                     EFEGDLRLVAAAQGLAVTPSHALEHGFLSGQHRSKSAMSGVRGQQLRSQLNRRGIRVL
                     RALDVIAAELSVPVAAVSIAWLLAQRTVVAPIVNAFATTHVDELMQGAGITLSRAQIA
                     ELTRAGA"
     gene            complement(2799..5072)
                     /locus_tag="VN041_14390"
     CDS             complement(2799..5072)
                     /locus_tag="VN041_14390"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWU30269.1"
                     /translation="MEGPEITATEAVLDNGRFGTRTVRFETGRLAQQAQGSVAAYLDD
                     ETMLLSATSAGKHPREGFDFFPLTVDVEERSYAAGKIPGSFFRREGRPSTEAILVCRL
                     IDRPLRPSFVEGLRNEVQIVITVLSIAPGEFYDALAINAASASTQISGLPFSGPIAGV
                     RLAFIPGHGEHADQWVAFPNAAQVEEAVFDLVVAGRVVTKADGSEDVAIMMVEAEATE
                     NSWNLIKAGATKPSEDIVAQGLEAAKPFIAQLVKAQAELASHASKEPGVYPVFLPYSE
                     ETFAFVNERAHDELVGVYQIADKIERQSADDAIKDRVKGELAAAVEAGTLPESALAEF
                     SGAYKSVTKKIVRGRILTEGVRIDGRGLADIRPLDAEVQVIPRVHGSAIFQRGETQIL
                     GVTTLNMLKMEQQIDSLSPTTSKRYMHHYNFPPYSTGETGRVGSPKRREIGHGFLAER
                     ALVPVLPSREEFPYAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPLRAPVAGIAMG
                     LVSDEVDGETRYAALTDILGAEDALGDMDFKVAGTSEFVTAIQLDTKLDGIPSSVLAG
                     ALTQARDARLTILNVLNAAIDAPDEMAPTAPRVISVQIPVDKIGELIGPKGKTINAIQ
                     DETGAQISIEEDGTVYIGAVDGPSAEAARAQVNAIANPTNPEVGEQFLGTVVKIASFG
                     AFISLLPGKDGLLHVTEVRKLAGGKRVENVDDVLSVGQKILVKITKIDDRGKLSLEPV
                     LDDAPAEGEKVEEPAEA"
     gene            complement(5334..>5741)
                     /locus_tag="VN041_14395"
     CDS             complement(5334..>5741)
                     /locus_tag="VN041_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWU30270.1"
                     /translation="ASAVYARRFAAYASALAAVAQRTGAILIDADLHPMLGRRPNWAE
                     DLVHLSSRGHRFLAYRVGEALGVPHAEALGVLDESLHDHEHIPRRTWWRRHALPWVWR
                     RLHGRTAGDGRVAKHDDWVYIARNGARVREHAG"
BASE COUNT          896 a         1922 c         2027 g          896 t
ORIGIN      
        1 ggcgagcagc gctctcatcg ccgcgccgcc cgcgtcgtgg ccggccacct gcacggggcc
       61 gagcacgccg acggggaagg tcatgccttc gggcgcaggc cgaggtcgat gatgttctcg
      121 agtccgatga ccaggtcgcg gttgtcgcgt gcgaacggaa ccgccagccg gatgccgggt
      181 gcataggcgg ctgcggagtc caccgtgtcg tgcacgatcg tcagggactc ccctgccccg
      241 gacagaatcg cctcctgccg ggcgatcact cccggacgac gcagcgagtg caccgggatg
      301 cctgcgacct gctgcccccg ggcgcgctga tcgacgtgcg gggcgtcgaa cggtccccgc
      361 tcctcgcgcg cttcggccat ctgctccgcg gttcgcaccg cagtgccgct gggagagtcg
      421 atcttggagt cgcgatgggc ttcgacgatc tcggcgaagg ggaagaacgg cgcggcggcg
      481 gccgccagcg ccgtgcccag cgcggagccc agcgagaagt tggggatgaa gaccacgccg
      541 gtcccggcgg aggcctgcag cgggcgaacc agcgacatgc gctcctggga ccagcccgag
      601 gtgccgacca ggatgctgat gccgcgctcg agggccgcgc ggaccacctc gatgctcacg
      661 gcaggggtgg acgcgtcgac gacgaggtcg gcgccgtcga gttcgctgag atcgctcgcg
      721 gagccgagga cattgcgcac ctcgaaaccg tcgagctcgt cgatcaccga gtggatgatg
      781 cgcccgagct tgcctgatcc gccgacgagg gcaacctgag tggtcatgtc accagtctag
      841 attcgccgcg cgagcgtctc cgacccgtga cgggcgcccg tcagaccgag gggacatcgg
      901 ggagacccgt gcgcagctcc atcggcagat ggccgagatc gttgtggctg atcagcgtcc
      961 agggtcggcc ctgcttctga gcgatcacgc tcagcccgca atgcgcctga ttcagcgtca
     1021 tccagcgcca gtccggcgca cccagcacct cgcggacgaa ccacgagatc acgaagttgt
     1081 gcgtgatgag cacctcgtgc acctcgccgt tgcggcggcg gaggaactcg cccaccgcgt
     1141 ccgacatctg cgccgagccg gcctcgatct cggcctcgct gaccgacccg aagaaggact
     1201 cgtaagccgc aggtgtgtcc tcggtcatgc cagtcggaac gcagtcgaac agcagcgccg
     1261 aaggcacggg gtcgaccgag ggcagccgct ccgcgatcgc gcgcgcggtc tccgttgcac
     1321 gcagcagcgg ggagtgccag acggcatcca gcggaaggcc cgagagacgg tcggcgatga
     1381 gctcggcctg ccgcctacct cgagccgaga ggggtccgtc gtcgatgccg tgctcggcat
     1441 cctgatgttc accgtgtctg accagatata tgtagtgcgt cacaaccctg ctcgcttcgt
     1501 gccgccgcat cgcggcttct ccagcttagc cgcccgaccg acacccgcac cggtgaagtc
     1561 ggatcaggcg cccgcacggg tgagttcggc gatctgcgca cgtgagagcg tgatgcccgc
     1621 tccctgcatg agctcatcga catgcgtggt ggcgaacgcg ttgacgatcg gcgccaccac
     1681 tgtccgctgg gccagcagcc aggcgatcga caccgccgca acaggtacgg agagctcggc
     1741 ggcgatcacg tccagcgccc ggagcacgcg gatgccgcgg cggttcagct ggctgcgcag
     1801 ctgctgccct cgcaccccgc tcatggcgga cttgctgcga tgctgtcccg acaggaaccc
     1861 gtgctccagt gcgtgcgaag gcgtgaccgc cagcccctgg gcggcggcca ccagtcgcag
     1921 atcgccctcg aactcggtgc ggcgcaccag gttgtacggc tcgtccagca cgtcgagacg
     1981 gggatacccg gctgaggcca ggatgcgcgc ctcgaccagg cgctcgggcg cgaagctgaa
     2041 cgcgccgacg gacgcgatct tgccggcgtc ccgcagccat tcggtcgttg ccagggtgtc
     2101 ctcgaggttc gtggtctcgt ccagcgacgc gtccagatac agcacgtcga tccgctcgac
     2161 gccgagccgc gtcagcgacc cctcgacggc gcgtacgagg ttcaccgacc ccagcccggg
     2221 gttgtcggcg tgcgctccga tgcgcacact cagcacgagt ccgtcgtggt ccgcgcgcga
     2281 gcgcatccac tggccgatga tgtgctcgct gcgtcctccg gagaagccgt cggcggtgtg
     2341 gatcgcgttg cccccgaact cgacgtagcg gttcagcagt gcatggctgg tgggcaggtc
     2401 gacgttccac ccgaactctg cggcgcccag catgagcggg aagatcgaga agccggtctc
     2461 ccccagtggc acccggatgc cctcgccgac gccgggtccg acgacgggga tcggcgcgga
     2521 gggatgctcg tccagggcct cgtcagggcg cgaccgctga tcggccgtgc cgactccgaa
     2581 gatcctcatc tctcaccccc gcgagatccg caacccaccc cggggtgcgt acttcgaggg
     2641 taagcgaggt tttccacggc gatgaacact gcgacagcgg cgacgagaag ttctcgtaac
     2701 gatccgatca catcggatac gacacagggc ccggagccgt gctcatgcac tgctccgggc
     2761 cctgctcacg gatgccgggt caccggcatc cgacgggatc acgcctcagc gggctcctcg
     2821 accttctcgc cctcggcagg cgcgtcatcg agcacgggct cgagggacag cttgccgcgg
     2881 tcgtcgatct tcgtgatctt gacgaggatc ttctggccga ccgagaggac gtcgtcgacg
     2941 ttctcgacgc gcttgccacc ggcgagcttg cgcacctcgg tgacgtgcag caggccgtcc
     3001 ttgccgggca gcagcgagat gaaggcgccg aagctggcga tcttcacgac ggtgccgagg
     3061 aactgctcgc cgacctcagg gttggtgggg ttggcgatcg cgttgacctg ggcacgggcg
     3121 gcctcggccg acgggccgtc gacggcgccg atgtagacgg tgccgtcctc ctcgatggag
     3181 atctgcgcgc cggtctcgtc ctggatcgcg ttgatcgtct tgcccttcgg gccgatcagc
     3241 tcaccgatct tgtcgaccgg gatctgcacg ctgatgacgc gcggcgcggt gggcgccatc
     3301 tcgtcgggag cgtcgatcgc ggcgttcagc acgttgagga tcgtgagacg cgcgtcgcgg
     3361 gcctgggtca gggcaccggc caggaccgac gacgggatgc cgtcgagctt ggtgtccagc
     3421 tggatcgcgg tgacgaactc gctcgtaccg gcgaccttga agtccatgtc gcccagcgcg
     3481 tcctcggcgc cgaggatgtc ggtgagggcc gcgtagcggg tctcaccgtc gacctcgtcg
     3541 gagaccaggc ccatggcgat gccggcgacg ggggcgcgca gcggcacacc cgcgttcagc
     3601 agcgacaggg tggacgcgca gacggagccc atcgaggtgg agccgttgga gctcagcgcc
     3661 tcggagacct gacggatcgc gtaggggaac tcctcgcgag agggcagcac cggcacgagg
     3721 gcgcgctcgg ccaggaagcc gtgcccgatc tcgcgacgct tcggcgaccc gacacgaccg
     3781 gtctctccgg tggagtaggg cgggaagttg tagtggtgca tgtagcgctt gctcgtggtg
     3841 ggagacagcg agtcgatctg ctgctccatc ttgagcatgt tcagcgtggt gacgcccagg
     3901 atctgggtct caccgcgctg gaagatcgcc gagccgtgca cgcgcgggat gacctgcacc
     3961 tctgcgtcca gcggacggat gtcggccagg ccgcgaccgt cgatgcgcac gccctcggtg
     4021 aggatgcggc cgcgaacgat cttcttggtg accgacttgt aggcgccgga gaactcggcc
     4081 agtgccgact cgggcagggt gcccgcctcg acggcggcgg cgagctcgcc cttgacgcgg
     4141 tccttgatcg cgtcgtcggc gctctgacgc tcgatcttgt cggcgatctg gtagacgccc
     4201 accagctcgt cgtgcgcgcg ctcgttgacg aacgcgaagg tctcctccga gtacggcagg
     4261 aagacggggt agacacccgg ctccttggag gcgtgcgagg cgagctcggc ctgtgccttg
     4321 accagctggg cgatgaacgg cttggcggcc tcgagaccct gcgcgacgat gtcctcgctg
     4381 ggcttggtcg cgccggcctt gatcaggttc cagctgttct cggtggcctc cgcctcgacc
     4441 atcatgatcg cgacgtcctc ggagccgtcc gccttggtga cgacgcgacc ggcgacgacg
     4501 aggtcgaaga cggcctcctc gacctgggcg gcgttcggga aggcgaccca ctggtcggcg
     4561 tgctcaccgt gaccggggat gaacgccagg cgcacacccg cgatcggacc ggagaacgga
     4621 agaccggaga tctgggtcga cgcggatgcc gcgttgatgg ccagcgcgtc gtagaactcg
     4681 cccggggcga tcgacagcac ggtgatgacg atctggacct cgttgcgcag gccttcgacg
     4741 aaggacgggc gcagcgggcg gtcgatcagg cggcagacca ggatcgcctc ggtggaggga
     4801 cggccctcgc gacggaagaa cgagccgggg atctttccgg cggcgtacga gcgctcctcg
     4861 acgtcgacgg tcagcgggaa gaagtcgaag ccttcgcgcg ggtgcttgcc tgcgctggtg
     4921 gccgacagga gcatggtctc gtcgtcgagg tacgcggcga cggagccctg cgcctgctgg
     4981 gcgaggcgtc cggtctcgaa ccggacggtg cgggtgccga agcggccgtt gtcgagaacg
     5041 gcctcggtgg cagtgatttc aggaccttcc aagaggtctc tccttctttg tttaggctcg
     5101 cgcgtccgta tgacgcgcga gttctcgcga aggagcggat gcgggcagat ataagcgcgc
     5161 gggcattccc gcgaatctcg cctgcgctgg ccaccagtag aagatcaccc ggcgccgtgt
     5221 gcgacgagga acccaccaca ggggaccagc ttctcagccg accgctccgt actaagtcga
     5281 tatgcagttt gacggatgcc acaggcatcc tccataagcc taccagtgcg atctcaaccg
     5341 gcgtgttcgc gcacgcgggc gccgttgcgc gcgatgtaca cccagtcgtc gtgcttggcc
     5401 acgcgtccgt cgcccgccgt gcgtccgtgc agccgccgcc agacccaggg cagcgcgtgc
     5461 cgtcgccacc acgtgcggcg cgggatgtgc tcatggtcgt gcagggactc gtccagcacg
     5521 cccagcgcct cggcatgcgg cacgcccagc gcctcaccca cccggtaggc gaggaagcgg
     5581 tggccgcggc tggagaggtg cacgaggtcc tcggcccagt tcgggcgacg tccgagcatc
     5641 ggatgcagat ccgcgtcgat caggatcgca ccggtgcgct gcgcgactgc ggcgagcgcg
     5701 ctggcgtatg cggcgaagcg ccgggcgtag accgcggagg c
//
LOCUS       DATMSX010000450         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_177435,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000450 DATMSX010000000
VERSION     DATMSX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177435"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..714)
                     /locus_tag="VN041_14400"
     CDS             complement(37..714)
                     /locus_tag="VN041_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HWU30271.1"
                     /translation="MRRMLRDNALGLFFLLLFLLALAGQSVAGYFRQVEEMTEHGRAP
                     ADFLAFVLSPDFVVDVAENWQSEFLQFFLFIGATVWLVQRGSPESKKPGDEGPESDED
                     QLVGRYAPDRAPRWARASGWRRTVYGNSLLIVMGTVFALSWLAQSLGGTIVAGEEAAM
                     HGMPAPSWAEYVVSADFWDRTLQNWQSEFLAVGTMVAFSIYLRQRGSAESKPVGTPHA
                     VSAVESE"
     gene            complement(717..857)
                     /locus_tag="VN041_14405"
     CDS             complement(717..857)
                     /locus_tag="VN041_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30272.1"
                     /translation="MSQVDSRETVERGEPAGRGPAVVFWVWMTVTFGGLAIMFAIIIG
                     GR"
     gene            1021..1449
                     /locus_tag="VN041_14410"
     CDS             1021..1449
                     /locus_tag="VN041_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWU30273.1"
                     /translation="MTLTTEVLVKDIMTPGARCIGENDSLETAARLMADLDIGSLPIC
                     GENGKLKGMLTDRDIVVKAIAHGRSAAETRAGALAEGTPLLVDAEDGVSAVLELMQKH
                     QVRRIPVLDDHVLVGMVAEADIARSLAPQHTGETVEQITR"
     gene            1476..2030
                     /locus_tag="VN041_14415"
     CDS             1476..2030
                     /locus_tag="VN041_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWU30274.1"
                     /translation="MDADTDATENESRTHADTEAMAKDMSRRPADATTTHAAWGSGDP
                     HLLVSREDVRNVFPLTADETRIGSAPDCELVLDGADPVHATIVHDDRDEYVLTMHGEG
                     EMNAWSEDDTRTEILRTGARFTVAGWSLVFMREEYADHGRPWGGREGGELSDQPPQPQ
                     RPDYAAQRSGEGDEDSGGWEVRNG"
     gene            complement(2049..2252)
                     /locus_tag="VN041_14420"
     CDS             complement(2049..2252)
                     /locus_tag="VN041_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30275.1"
                     /translation="MTDPEIHTWWPLLSADAKHTLEALDGEIIPDLVREEVEALTGVR
                     MPREERLSERDWDFIRTQREAVD"
     gene            2465..2938
                     /locus_tag="VN041_14425"
     CDS             2465..2938
                     /locus_tag="VN041_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30276.1"
                     /translation="MAEKLHGYAPTTTHAAWGAGDPHLLISRNDEDRSVLHLGAETVT
                     IGSDEECTVRLEGAAPLHATIVHDDRDEFVLTMHGPGRMNASSPEGERGERVETLRTG
                     AQFTIGDWMLVFSRDEYADHGRPYGGRNGGEGTHQERQPERPDYPATGEIPVHDR"
     gene            2996..3271
                     /locus_tag="VN041_14430"
     CDS             2996..3271
                     /locus_tag="VN041_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6412 domain-containing protein"
                     /protein_id="HWU30277.1"
                     /translation="MIQTLLQLLLTALRVVPLPAGTSAGGMLGIAAVLIAVAVLAIAL
                     ALVRPIFGIHSVPHPRRAIDVSALLAQSDPDAAGHPRPRAPGAAFAA"
     gene            3347..4084
                     /gene="yidC"
                     /locus_tag="VN041_14435"
     CDS             3347..4084
                     /gene="yidC"
                     /locus_tag="VN041_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWU30278.1"
                     /translation="MDIFAFPPLALLLDGAYTALLSLSHLLEPFAGAAAAALAVVLVT
                     LLVRTCLIPVGVSQAKAEQMRARLAPRMRELQQKHGKDRESLQRETMALYRDEKASPF
                     AGCLPLLVQAAVVGILYTLFLRPEIAGHANALLSHDLFGAPLGISLLGAIGTGIDAAT
                     AIVWAVALLIIVVIADITRRVFPAQVPDEGPLAVPGMEGTMNALHYLTAVFAVFVPLA
                     AALYLVVTVTWTLVQRAVLRRRYPLPA"
     gene            complement(4101..>4472)
                     /locus_tag="VN041_14440"
     CDS             complement(4101..>4472)
                     /locus_tag="VN041_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWU30279.1"
                     /translation="RSAAADAGGREPDIVIEASGHAVTDAIEGVAVGGTAVLVGSVFP
                     ADPVPLDAESVVRRLVTVTGVHNYTGRDLSDAVSFLAGPGRVHPFAEVVSAVHPLSEL
                     DRALAAASAPDAPLRIAVAAR"
BASE COUNT          710 a         1618 c         1533 g          611 t
ORIGIN      
        1 gaccacggcc gcgaccgctg agcgggccgc gcccggtcat tccgactcca ccgccgacac
       61 ggcgtgcggc gtgccgaccg gcttggactc cgcagagccg cgctggcgca ggtagatcga
      121 gaatgcgacc atcgtgccga ccgcgaggaa ctcggactgc cagttctgca gagtgcggtc
      181 ccagaagtcg gcagacacca catactcggc ccaggacggc gcgggcatgc cgtgcatggc
      241 cgcctcctcc cccgcgacga tcgtgccgcc gagcgactgc gccagccagg agagggcgaa
      301 gaccgttccc atcacgatca gcagggagtt gccgtacacc gtccggcgcc agcccgatgc
      361 ccgcgcccag cgcggcgcac ggtcgggtgc ataccgcccg accaactgat cctcgtcgct
      421 ctcaggtcct tcgtcccccg gcttcttcga ctcgggtgac ccgcgctgca cgagccacac
      481 ggtcgcgccg atgaacagga agaactgcag gaactccgac tgccagttct ccgccacgtc
      541 caccacgaag tcgggcgaga gcacgaacgc gaggaagtcg gcgggtgccc gcccgtgctc
      601 cgtcatctcc tcgacctgcc ggaagtagcc ggcgaccgac tgacctgcga gcgccagcag
      661 gaacagcagt aggaagaaca gtcccagcgc gttgtcgcgc agcatccgcc gcatggtcat
      721 cgacctccga tgatgatcgc gaacatgatc gccagacctc cgaaggtcac cgtcatccac
      781 acccagaaca ccaccgccgg gccgcgcccg gccggctcgc ccctctccac cgtttccctg
      841 ctgtccacct ggctcatccg gtcctcctca ctgtcaagac gagacgactt cccgatgctg
      901 gccgggcgac agccggatgc ccagcccctt gacgccggcg gcgcggcctg tcaaccccct
      961 cagcgacccg ccgagcactc cgtatcctcg gccttccgag caccaggaga ggaagagatc
     1021 atgacgctga cgacggaggt cctggtgaag gacatcatga cgccgggggc acgctgcatc
     1081 ggcgagaacg actcgctgga gaccgcggcc cgcctgatgg ccgatctgga catcggctct
     1141 ctgcccatct gcggggagaa cggcaagctg aagggcatgc tgaccgaccg tgacatcgtc
     1201 gtcaaggcga tcgctcacgg tcgctccgcc gcggagacgc gggcgggcgc cctcgccgag
     1261 ggcactcctc tgctcgtcga cgccgaggat ggagtgagcg cggtgctcga actcatgcag
     1321 aagcatcagg ttcgccgcat cccggtgctc gacgaccacg tgctggtcgg catggtcgcc
     1381 gaggccgaca tcgcacgcag cctcgccccg cagcacaccg gcgagaccgt cgagcagatc
     1441 acgcgctgag cagacccgca cggaaggaga cagacatgga tgctgacacc gacgcgacgg
     1501 agaacgagtc ccgcacgcac gccgacaccg aggcgatggc gaaggacatg agccggcgtc
     1561 ctgccgacgc caccaccacc cacgccgcct ggggctcggg cgatcctcac ctgctcgtct
     1621 cccgggagga tgtcaggaac gtcttccccc tgacggcgga cgagacgcgg atcggctcag
     1681 cgcccgactg cgagctcgtg ctggacggcg ccgatccggt gcacgcgacc atcgtgcacg
     1741 acgaccgcga cgagtacgtc ctgacgatgc acggcgaggg cgagatgaac gcctggtccg
     1801 aggacgacac ccgcacggag atcctccgca cgggcgcgcg cttcaccgtg gccggctggt
     1861 cgctggtgtt catgcgcgag gaatacgccg accacggccg cccgtggggc ggccgtgagg
     1921 gcggtgaact ctccgatcag ccgccgcagc ctcagcgccc ggactacgcc gcgcagcgct
     1981 ccggcgaagg ggatgaggac agcggcggct gggaggtgcg caacggctga ggcctcaggg
     2041 cccaggcctc agtcgaccgc ctcgcgctga gtgcggatga agtcccagtc gcgctcgctc
     2101 agccgctcct cgcgcggcat ccggacgccg gtcagggctt ccacctcttc ccgcacgagg
     2161 tcggggatga tctcgccgtc cagggcctcc agcgtgtgct tcgcgtccgc ggacagcaac
     2221 ggccaccagg tgtggatctc ggggtcggtc atttcggact cctctcgcga tgtgccgatg
     2281 ctcgcatctg acgttccctc ggcgcagggg gttgacagcc cggcatccgc agatcaccgc
     2341 ccggtggggt gtggacgggc gtatcgtcga gacagaggct tacgcgacct tctggcgtca
     2401 ggagggtcga cgagagcacc ttcccgccgg cccgaggagt catccacgga taggaggaag
     2461 ggtcatggct gagaagctgc acggatacgc gccgacgacg acgcacgccg cctggggtgc
     2521 gggcgatccg catctgctga tctcccgcaa cgacgaggac cgctcggtgc tccatctcgg
     2581 agcggagacc gtgacgatcg gctcggacga ggagtgcacg gttcggctgg agggggcggc
     2641 gcccctgcac gccacgatcg tgcacgacga ccgcgacgag ttcgtgctca cgatgcacgg
     2701 cccggggcgg atgaacgcca gcagcccgga gggcgagcgc ggtgagcggg tggagacgct
     2761 gcgcaccgga gcgcagttca cgatcgggga ctggatgctg gtgttcagcc gtgacgagta
     2821 cgcggaccac ggccggccct acggcgggcg caacggcggt gagggcaccc accaggagcg
     2881 tcagcccgag cgccccgact acccggcgac cggggagatc ccggtccacg atcgctgagc
     2941 tccgtcgacc tgcaggggcg gctggtagcc tcggcctgag ggaaggggga cgaccatgat
     3001 ccagacgctg ctgcagctgc tgctgaccgc gctgcgcgtg gtgccccttc cggccgggac
     3061 ctcagcgggc gggatgctgg gcatcgccgc ggtcctcatc gcggtcgccg tgctcgcgat
     3121 cgccctggca ctggtccggc cgatcttcgg catccactcc gtgccgcatc cgagacgcgc
     3181 gatcgacgtg tccgccctgc tcgcgcagag cgatccggat gccgcggggc atccgcgtcc
     3241 gcgagccccg ggagccgcgt tcgccgcgtg accccgccga cgccctgagc gtccgcagcg
     3301 ggacaccgcg gcatccgccg acctcccgcg accacgaacg gacaccatgg acatcttcgc
     3361 cttcccgccg ctcgccctcc tcctggacgg cgcctacacc gcactgctct cgctctcgca
     3421 cctgctcgaa ccgttcgccg gcgccgcggc ggccgcgctc gccgtcgtgc tcgtgacgct
     3481 gctcgtgcgc acctgcctca tccccgtcgg cgtctcgcag gcgaaggccg agcagatgcg
     3541 cgcccgcctc gcccccagga tgcgggagct gcagcagaag cacggcaagg accgcgagag
     3601 cctgcagcgc gagaccatgg cgctgtaccg cgatgagaag gcatcgccgt tcgccggatg
     3661 cctgccgctg ctcgtgcagg cggccgtcgt cggcatcctg tacacgctgt tcctgcgtcc
     3721 cgagatcgcc gggcacgcga acgcactgct gagtcacgac ctgttcggcg ccccgctcgg
     3781 catctcgctg ctcggggcga tcggcaccgg tatcgacgcg gccaccgcga tcgtgtgggc
     3841 ggtggcgctg ctgatcatcg tggtcatcgc cgacatcacc cgacgggtgt tccccgcgca
     3901 ggtgccggac gaaggtccgc tggcggtgcc gggcatggag ggcacgatga acgccctgca
     3961 ctacctgacc gcggttttcg ccgtgttcgt gccgctcgcc gccgcgctct acctggtggt
     4021 gaccgtcacc tggacgctgg tgcagcgcgc ggtgctgcgc cgcaggtacc cgctgccggc
     4081 gtgaggcggg tcggcgcggc tcagcgtgcg gcgacggcga tgcgcagcgg cgcgtcggga
     4141 gcggatgccg cggcgagcgc gcggtcgagc tcggagagcg gatgcacagc gctgaccact
     4201 tccgcgaacg gatgcacccg gccggggccg gcgaggaacg agaccgcgtc ggacaggtcc
     4261 cgcccggtgt agttgtgcac tccggtcacc gtgaccagcc ggcgcacgac ggactcggcg
     4321 tcgagcggaa cggggtcggc ggggaacacc gaacccacga gcaccgcggt cccgcccacc
     4381 gcgacgccct cgatggcgtc ggtgacggca tggccggagg cctcgatcac gatgtccggc
     4441 tcgcgaccac cggcatccgc cgccgcagat cg
//
LOCUS       DATMSX010000451         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_54291,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000451 DATMSX010000000
VERSION     DATMSX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54291"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VN041_14445"
     CDS             complement(<1..250)
                     /locus_tag="VN041_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30280.1"
                     /translation="MSSEPAAVFTPPSRVRGTLALVAGRDETAAVYRRFGSRISADGY
                     AVGVFGTADAASGWLSAQDGAPRVLVGSDTGASAVLTAL"
     gene            371..1885
                     /locus_tag="VN041_14450"
     CDS             371..1885
                     /locus_tag="VN041_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30281.1"
                     /translation="MTSRNRLPALAVLAVATLAMAGCSASAGATPSENTSLTWGWRLP
                     TSWDPVTSTAGWDTHALALVYDGLTQLDPAGEVVPGIAESWDYSADGTAITFHLRKDA
                     EFSDGSPIDAEAVKENLERGRDQENSTLAGQLRIVTSVDVKDATTVTVHLDQADYQVP
                     YLFAGKTGFIVNPAAFKDVASLATQPEGSGPFELTEYVPNARADLQKNDHYWNADHIF
                     IDQFHVVPVTDDATVVAGIDTGQWDVAIIPPSQAKAAEGAGLDVEEIKALTVRALDVN
                     STVAPFDDQRVLQAISHATDRKALIKAAYFGRGTPNWQPFPDGYSAHDKSLDDLYPYD
                     VAAARKLLAESGHPDGLKLELALSEDDTALAEVLQAQWAKAGIDVKLSVLPATAISQN
                     YVQRTHPFVLDSFSGRQSPLQALEVLYGPEGLMNLGRQTPAELTAAIDAARATPTDSP
                     EYEKRIQHAVELGVKLQPNTFLFSWPRILAHPSKVTGIRHWLDVQRWEDVKVKG"
BASE COUNT          328 a          717 c          649 g          260 t
ORIGIN      
        1 tgagcgcggt gagcaccgcc gacgctccgg tgtcggagcc gaccagcacg cgaggggcgc
       61 catcctgggc ggagagccag ccggaggccg cgtccgcggt cccgaacacg ccgacggcgt
      121 acccgtcggc gctgatgcgc gatccgaagc ggcggtacac cgcggcggtc tcatcgcgcc
      181 cggcgaccag cgcgagcgtc cctcggacgc gggagggcgg ggtgaagacg gcggcgggct
      241 cggaactcat gcgtcccatc ttcgggacag gtgctcgggt gcgcctcgga tgtgtcgaac
      301 tgttgcgtac cgtgacctcg cgcttcagat cgcttgtggt cccggcttcc gcgctcctag
      361 cttcactgcc atgacctcac gcaaccgact ccctgccctc gccgtgctcg ccgtcgcgac
      421 cctcgcgatg gccggctgca gcgcgtcggc gggtgcgaca ccctcggaga acacctcgct
      481 cacctggggc tggcgcctgc cgaccagctg ggacccggtc acctcgaccg ccggctggga
      541 cacccacgcg ctcgccctcg tctacgacgg actcacccag ctcgaccctg ccggcgaagt
      601 cgtccccggc atcgccgaga gctgggacta cagcgcggac ggcacggcca tcaccttcca
      661 cctgcgcaag gacgcggagt tcagcgacgg cagcccgatc gacgcggagg cggtcaagga
      721 gaacctcgaa cgcggccgcg accaggagaa ctccaccctg gccggccagc tgcgcatcgt
      781 gacctcggtg gacgtgaagg atgcgaccac ggtgaccgtg cacctggacc aggccgacta
      841 ccaggtgccc tatctgttcg ccggcaagac cgggttcatc gtgaacccgg ccgcgttcaa
      901 ggacgtcgcc tcgctcgcca cccagcccga gggctcggga ccgttcgagc tcaccgagta
      961 cgtccccaac gcccgcgccg acctgcagaa gaacgaccac tactggaacg ccgaccacat
     1021 cttcatcgat cagttccacg tcgtgccggt caccgatgac gcgaccgtgg tcgccggcat
     1081 cgacaccggc cagtgggatg tcgcgatcat cccgccctcg caggcgaagg cggccgaggg
     1141 tgcgggcctg gacgtcgagg agatcaaggc gctcaccgtg cgcgcactgg atgtcaacag
     1201 cacggtcgcg ccgttcgacg accagcgcgt gctgcaggcg atcagccatg cgaccgaccg
     1261 caaggccctc atcaaggccg cctacttcgg tcgcggcacg ccgaactggc agcccttccc
     1321 ggacggctac tccgcgcacg acaagagtct cgacgacctg tacccgtacg acgtcgccgc
     1381 ggccaggaag ctgctggccg agtccgggca tccggacggg ctgaagctcg agctcgcgct
     1441 cagcgaggac gacacggcgc tcgccgaggt gctgcaggcg cagtgggcga aagccggcat
     1501 cgacgtgaag ctcagcgtgc tccccgccac ggcgatctcg cagaactacg tgcagcgcac
     1561 ccatcccttc gtgctggaca gcttctccgg acggcagtcg ccgctgcagg cgctcgaggt
     1621 gctctacgga cccgaggggc tgatgaacct cggccggcag acgccggcag agctcaccgc
     1681 ggccatcgat gccgcacggg cgacgcccac ggactccccg gagtacgaga agcgcatcca
     1741 gcacgccgtc gagctcggcg tgaaactgca gccgaacacg ttcctgttca gctggccgcg
     1801 catcctcgcg cacccgtcga aggtcaccgg catccgtcac tggctcgacg tgcagcgctg
     1861 ggaagacgtc aaggtgaagg gctgaccatg agcgtcgtca ccgagccggg cacggagacg
     1921 gggcccgccg caggcgcggc acccgccgtg cggc
//
LOCUS       DATMSX010000452        16414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_139653,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000452 DATMSX010000000
VERSION     DATMSX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 16414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16414
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139653"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VN041_14455"
     CDS             <1..854
                     /locus_tag="VN041_14455"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU30282.1"
                     /translation="AHADVAPAYGLSGLEGLRAAQQKLKGVLDMQVVGFPQHGVLRAP
                     GTAELLERAAAEGLLDYVGGIDPTAFEGADRASEQLDVLFRIATSHSVGLDMHIHESG
                     GLGIATIRDILARARAAGLTGGEVTISHAYAIASLPISEQERIADEIAAVGVKLTTVS
                     PSPSSVLPHRRLREHGVHVGLGSDGVRDNWSPFGNADMLHRTWILGWSTGARLDDELE
                     SGFDLASAGGAEVMELPESTLKVGAPADLMVIDGENIPQVVIDQPRRDYVLRGGVVVA
                     QDGEMVV"
     gene            complement(921..1274)
                     /locus_tag="VN041_14460"
     CDS             complement(921..1274)
                     /locus_tag="VN041_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30283.1"
                     /translation="MTVRLNEDALSHARGLLRSGDVVHDERDDWSEHAPSTDDENDFI
                     DKHGMGEFARWHLGEDTDATEGTKGRYSFPFGDFRKLHRCAVISAESRAGQYDHASIE
                     KAFKELLEKIDEKSD"
     gene            1455..1838
                     /locus_tag="VN041_14465"
     CDS             1455..1838
                     /locus_tag="VN041_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30284.1"
                     /translation="MTQQSSGRRTRRTRVPGSPSDPTLVTNQPALRSSSGAVWLISSG
                     VFVAVCLVPLIGIVAVRSAAAPVAIVAIVLLVGLLAAEFVVRFLISAPLQRLRWLAAC
                     MLSMAVVALIAMMVCVSIVWSSALH"
     gene            complement(1853..2416)
                     /locus_tag="VN041_14470"
     CDS             complement(1853..2416)
                     /locus_tag="VN041_14470"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HWU30285.1"
                     /translation="MNAPLIVEYSAIPPTPWKNGRGVTRNLFDDATDAGVWTWRVSIA
                     EITGTQPYSPYPGVRRGQVALGPGAVDLRIDGRDLVLDPEGIVVFDGEAEVTASPREA
                     GFLDLNVMTRRDTWESDVQCVDDPGVTEAGDGIVILVALGDTALVDGRSLARLDSVML
                     GASASASADVRGRFAVARIAARRHPLG"
     gene            2460..3203
                     /locus_tag="VN041_14475"
     CDS             2460..3203
                     /locus_tag="VN041_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWU30286.1"
                     /translation="MSPSRRVGKRLIVIAASTVAALGILAVLVFNGVLWPARLFAASY
                     PVRGIDISSYQGDVDWDELASQDIDFAYVKATEGSKSTDSRFSANWAGASRTDLLIGA
                     YHFVSFESTGEAQAAHIIETVPTGATLPIALDLEYYGDFFAHPPTRTQVDAILEPLLR
                     LLEEHYGSPPVIYATSSAYDRYLSGGYADNPIWIRSIVLPPTLDDGRDWTIWQYSDHD
                     RLRGYVGVEQYIDMNVFHGTREELAALAK"
     gene            complement(3226..3636)
                     /locus_tag="VN041_14480"
     CDS             complement(3226..3636)
                     /locus_tag="VN041_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30287.1"
                     /translation="MRLRRWVLPVVMLLSSASVAACSSSEPAFPEAQVAPPGDAWVVV
                     VFDDTDLEFDLGVMDALLETELAADDALTEAGMGMIDGNEVGDHSYELYFVGGEGEAM
                     WRVLEPVFEDAPVPWTRVEIRSGLEDEAPRVITR"
     gene            3711..4259
                     /locus_tag="VN041_14485"
     CDS             3711..4259
                     /locus_tag="VN041_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWU30288.1"
                     /translation="MNICVFLSASDLDERYTRAAQQLGRLIGERGHTLVWGASDVGLM
                     KVLADAVDSAGGRLVGISVGFLAAKARQLQSPNELIVGADLAERKATLLTRSDAVVIM
                     VGGSGTLDEATDVLELRKHGIYDGPVVVLNTDGFYDGLQQQFARMDAEGFLPVPLAQL
                     CTFVDQPEEALAVIEQHAPTRR"
     gene            4339..4821
                     /locus_tag="VN041_14490"
     CDS             4339..4821
                     /locus_tag="VN041_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWU30289.1"
                     /translation="MENVTGGEASTIDEQTFIVTRSISIAAPIEKVWRAITEPEHISR
                     WFGQTVFDGAGRGAQGTMTFDGHGTVPIRIEQIDAPRAVTYRWCNDNALDSPPSTLDE
                     AHSTVFTFTLEVEGAGTRLTVVEAGFESTSAPLLNIQRHRVGWDEELDKLIALFEVPA
                     "
     gene            4818..5132
                     /locus_tag="VN041_14495"
     CDS             4818..5132
                     /locus_tag="VN041_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU30290.1"
                     /translation="MTRTLVPVLAALGDDTRWSILAALGEGDASASALAQRLPVTRQA
                     IAKHLAILSDVGVVEPVRVGREVRYRVVGATLSATANRLDAIGREWEQRLAAIKEIAE
                     DL"
     gene            5222..6016
                     /locus_tag="VN041_14500"
     CDS             5222..6016
                     /locus_tag="VN041_14500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30291.1"
                     /translation="MLLEDKVAVIHGGGGSIGAATARAFAREGARLFLAGRSLPRMEA
                     AAEEARAAGAKVEVAVVDASDQAAVDHHADEVAETAGRIDIALNAVGFDHAQGLAIAE
                     TSLEEYLYPIMGYLQTNFVTAKAAARHMVAQRSGVLLTVSTPGARLSGRGLIGNAAQS
                     AGLEGFSRALAGELGPDGVRVVCVRPHAISDALTTSYTHEMFGRIASASGLPMDDWLD
                     GLAGTTALGRLPVLDEVAEYLAFAASDRAASMTGVIANLSAGALVD"
     gene            complement(6106..6177)
                     /locus_tag="VN041_14505"
     tRNA            complement(6106..6177)
                     /locus_tag="VN041_14505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6142..6144),aa:Gln,seq:ctg)
     gene            complement(6277..6771)
                     /locus_tag="VN041_14510"
     CDS             complement(6277..6771)
                     /locus_tag="VN041_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWU30292.1"
                     /translation="MTDDVRSVPALELRRYLGLWFEVGRLPLRFEDDRATDVTAEYTM
                     AEDGTVTVDNRCRDEDGKPTRALGQAVPDAEHPGRLQVTFLPEALRWIPFTKADYWVL
                     RVDDEYRHALVGTPDRKHLWLLSREPQVEDAVERSFLDTARDQGFDLDPWIRPAQSGQ
                     VVAV"
     gene            complement(6864..8171)
                     /locus_tag="VN041_14515"
     CDS             complement(6864..8171)
                     /locus_tag="VN041_14515"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HWU30293.1"
                     /translation="MTDAAPPAVETDVILGDLGVLLLECGVSVTDVRGSLEQVGRRAA
                     STPQASLEFAILPEMVMVSRPGASSATTTVIGKGEALTFRQSAKASRLVHGLESGAVP
                     LAEAPARMAAIRATPRHRPALQSVIGCGLLSLSLAWLFRCPWWAIVLALLVGLLVGGL
                     TMLMMRVRAAAAVAPFVSAFVSTILVGTVAGWLDLGPVPLFAVCAPVAILVPGALITN
                     ALLELTSTDIVTGASRLMYGLIMLAFMAAGVYSGALLTGLRVDSASAALVGEAVELTT
                     DRGGWEALPPLWSAWLAVIVLAIGIGLAFGSGFRLTVVCVVVMAGTYAVLALFSPVVG
                     SVVATGIAAAVLFVAARVLERLTIAIPATVSFQPAFLLLVPGTVGLVSLASFDAQALV
                     SAPMMFLSLCIGTKVGALLADLARITRSTVFLRWAKPARMGEL"
     gene            complement(8177..9685)
                     /gene="gltX"
                     /locus_tag="VN041_14520"
     CDS             complement(8177..9685)
                     /gene="gltX"
                     /locus_tag="VN041_14520"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWU30294.1"
                     /translation="MATVHPLTTTATGSDIRVRFCPSPTGLPHVGMVRTALFNWAYAR
                     HNGGKLIFRIEDTDAARDSEESFRQLVDALTWMKIDWDEGVEVGGPHAPYRQSQRHDI
                     YREVIEKLKASGAVYESFSTAEEIDARNEANGRAKQLGYDNFDRDLTEEQKASFRAEG
                     RQPALRLRVPDEDLTYVDLIRGEVTFPAGSFPDYVIVRPNGVPLYTFVNPVDDALMGI
                     THVLRGEDLMPSTARQLVLYDALTKAGVTDFVPRFAHMPLVLGETGNKKLSKRDPQAD
                     LFLHRERGFIHEGLLNYLALLGWSIGPDRDVFSLEEFIQAFDIVDVNPNPARFDQKKA
                     ESINGDHIRQLDGKDFAERMLPYLADAGVFGAAEPTHEQIVLAFRAAPLVQERMQLLG
                     EAPGMLGFLFTDEVSYEADAMKGLPANAAEVLAACVAALEPVEEFTPDAVQTALSAKL
                     VDELGLKPRVAYGPPRVALTGRRVSPPLFESMELLGKDETLRRLRALADTLG"
     gene            complement(9730..11019)
                     /locus_tag="VN041_14525"
     CDS             complement(9730..11019)
                     /locus_tag="VN041_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30295.1"
                     /translation="MSAALTLDELAAIQRRTVRILSLGQVLGGIAFGATVSLGALLAA
                     DLSGSDALSGLATASVTLGAAAFAIPLARFAARRGRRLSLTAGNLLALIGVATVITAA
                     ATRLFPLLLIGIIMIGMGNAGNLQSRFAATDLAAPAHRARDLSVVVWSTTVGGVTGPL
                     LLTPGEALGHAIGMPPLTGSYLFSLVAQIAALILYLVALRPDPLLTALHLAKTTTTAS
                     PLGTGVDRPVVARYAMFAVAGSHAVMASVMAMTPIHLSHMAHGAHGSMPSSTDVTLLV
                     GITIALHVAGMYGLSPLFGILSDRWGRVRVVLLGQAVLAASLLTAIFAGRTPAGVMIA
                     LILLGLGWSAATVAGAALLTESSTVTMRPRRQGISDSLMSLTAAASAAAAGWVLTTFD
                     YAGLGIVALVFVIAITALSPLGRIRETAVEADPAATA"
     gene            complement(11016..11792)
                     /locus_tag="VN041_14530"
     CDS             complement(11016..11792)
                     /locus_tag="VN041_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU30296.1"
                     /translation="MKIARFSHSDGIRFGIVDEGDLVVLAGDPMFAGFDTTGERVPLA
                     DAALLAPVIPRSKVVCVGRNYHEHAAEFGNVAPEEPLLFLKPNTSVIGPGDAILRPAI
                     SERTEFEGELVVVIGRIAKNIKAEDALDYVFGYTVGNDFTARDIQRKDGQWTRGKGFD
                     TFCPLGPVIETEFDAASARIETRVNGEVRQQAPLTDMIHSVADIIAYASAAFTLLPGD
                     VIMTGTPAGVGPVVAGDTIEVEVSGIGTLRNPVRDAVPVS"
     gene            complement(11819..12907)
                     /locus_tag="VN041_14535"
     CDS             complement(11819..12907)
                     /locus_tag="VN041_14535"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWU30297.1"
                     /translation="MTDTTTAPLTFAVTKNLAAKSPAQREEILQNPGFGLHFTDHMVD
                     ICWSENGGWHRPRVQPYGPISLDPAAAVLHYGQEIFEGIKAYRHADGSVHTFRPDRNA
                     ARLQRSARRLALPELPTEHFIQSIRELVAVDGAWVPSGADQSLYLRPFMFAKEAFLGV
                     RPAKKVAYYLIASPAGAYFSGGVKPVRIWLSENYARAGKGGTGAAKTGGNYASSLLPQ
                     AEAYEKGCDQVVFLDQDNNVEELGGMNVVFVYKDGRVVTPESDSILEGITRDSLLQLA
                     EDRGLTVERRPVSLDEWRQGVASGDIVEVFACGTAAVVTPIGALVADGFEDAQPLGDL
                     ALSLREELTDIQYGRREDKHGWLLRLDG"
     gene            complement(12954..14003)
                     /locus_tag="VN041_14540"
     CDS             complement(12954..14003)
                     /locus_tag="VN041_14540"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWU30298.1"
                     /translation="MSRVVKLAVIPGDGIGREVIAQAERVLDAVTAGSDVAFEKTPFS
                     LGADRFLATGDTLTDADLDAIRSHDAILLGAVGGVPGDPRLKDANIERGLLLKLRFEL
                     DHYVNLRPSKLFAGAPGPLADPGEIDFVVVREGTEGPYVGNGGSIRKGTPHEVANETS
                     VNTAFGVERVVRYAFDLAERRRRKLTLVHKTNVLVHAGAIWKRIVDQVATEHPDVTVD
                     YLHVDAATIFLVTDPARFDVIVTDNLFGDILTDLAGAVTGGIGLAASGNINPDGAFPS
                     MFEPVHGSAPDIAGQQKADPTAAVLSVALLLDHLGLRDEAARVQRAVEQDIAARSGSR
                     STSETGDAVIALLQA"
     gene            complement(14122..15666)
                     /locus_tag="VN041_14545"
     CDS             complement(14122..15666)
                     /locus_tag="VN041_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30299.1"
                     /translation="MPATAPIELTGVDGPRVGLRGWAALAVLMLPVLLVSVDNTVLAF
                     ALPEISVSLQPSGAEQLWIIDVYSLVLAGLLVTMGVLGDRFGRRRMLLIGATGFAAVS
                     ALAAFAPSAGLLIAARAALGVFGAMLMPSTLSLLRSIFRNRDQRRLAIAVWASAFSAG
                     SALGPVVGGFLLEHFGWGSVFLIAVPVLIPLLIGGLWLLPESSDPNPGRIDPIGIVLS
                     MAAMIPVVFAIKSFAVDGPNLAAAIFLLVGVFAGFLFVRRMRRAENPMLDMTLFRRGG
                     FSGAILVNLLSVIALVGFLYFVSQQLQLVIGLSPMVAGLALVPGMVAMIIAGLGVVPI
                     SRRVAPHIVVPVGLSLSALGYVIVAATAGLNSVGWLLIAFMVLGVGIGAAETISNELI
                     LAHAPAEKAGAASAVSETAYELGAVLGTAVLGGIITAFYRGALVIPDGIPSELAAHAR
                     ETLAGAFTAAQELPGRLGSALWDAATAAFGSGVVTTSVIGALLVIGAIVVALTTLKPS
                     RNGSAG"
     gene            15767..16327
                     /locus_tag="VN041_14550"
     CDS             15767..16327
                     /locus_tag="VN041_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30300.1"
                     /translation="MSRPPLARERVLDAYESILIADGERAATLDATAQAAGVSKGGLL
                     YHFASKDDLAAGLAERLAVLTDQDLERMREAPDGAVAYYIRTSVMEDDALDRALIATT
                     RLAQGGSAAASAALREVRRRWADAIRPHVRDETALDLVMLLSDGLYFNNSLCEPVDGH
                     VSADDGFVPTGAALARLVELVRSSTA"
BASE COUNT         2648 a         5695 c         5687 g         2384 t
ORIGIN      
        1 gcgcgcacgc cgacgtcgcc ccggcctacg gtctgagcgg cctggaaggt cttcgcgccg
       61 cacagcagaa gctgaagggg gtcctcgaca tgcaggtcgt cggcttcccg cagcacggcg
      121 tgctgcgggc ccccggaacc gcagagctgc tcgagcgtgc ggccgccgag ggactgctcg
      181 actacgtcgg cggcatcgac ccgaccgcgt tcgagggcgc cgaccgcgca tccgagcagt
      241 tggatgtgct gttccgcatc gcgacctcac acagtgtcgg cctcgacatg cacatccacg
      301 agtcgggcgg gctcggtatc gcgaccatcc gcgacatcct cgcgcgcgca cgcgccgcag
      361 gcctcaccgg cggcgaggtg accatcagcc acgcctacgc gatcgcatcg ctgcctatct
      421 ccgagcagga gcggatcgcc gacgagatcg ctgcggtcgg ggtcaagctg accacagtct
      481 cccccagccc cagcagtgtg ctcccgcatc gccggctccg cgagcacggc gtgcacgtgg
      541 gcctggggtc cgacggagtc cgcgacaact ggagcccgtt cggcaacgcc gacatgctgc
      601 accggacgtg gatcctcggc tggtcgaccg gcgctcgcct ggacgacgag ctggagagcg
      661 gcttcgacct ggccagcgcc ggaggcgcgg aggtgatgga gctcccggag tccacgctga
      721 aggtgggcgc ccctgcggac ctgatggtca tcgacggcga gaacattccg caggtcgtga
      781 tcgatcagcc ccggcgcgac tacgtcctgc gcggcggcgt ggtcgtcgcc caggatggcg
      841 agatggtggt ctgagcgaag cgcgactgga ctcggggcgt cggatggcga gcatccggcg
      901 cccctgcgcc tgcgcggcgc ctagtcggac ttctcgtcga tcttctcgag cagttccttg
      961 aaggccttct cgatcgatgc gtggtcatac tgcccggcac ggctttccgc cgagatcacg
     1021 gcgcaacgat gcagcttccg gaagtcgccg aacgggaaac tgtagcgccc cttcgttccc
     1081 tcggtggcgt cggtgtcctc accgaggtgc catcgggcga actcccccat gccgtgcttg
     1141 tcgatgaagt cgttctcatc gtccgtgctc ggcgcgtgct cgctccagtc atcgcgctcg
     1201 tcgtggacga catctcccga gcgcagcagg ccgcgggcat gcgagagcgc gtcctcgttc
     1261 aatcggacgg tcatgttcca ctcctctccg cgcgcgtccc cgtctggacg tccgctgtct
     1321 ccgacgatgc ctcgggctgc ggtggtgccg aagggggtgg cacgcgccgc ccgggagtgc
     1381 taccggcata gcgcgaatcc agccccgcgt ccagcccgtc gacggcatcc gtctggtggc
     1441 ggatgctgag gcgcatgacc cagcagagct ccggacggcg cacacggcga acgcgtgtgc
     1501 ccggttcgcc gtccgatccg accttggtga ccaaccagcc ggcgctgcgg agttcgtccg
     1561 gagcggtgtg gctgatctcc tccggcgtgt tcgtggcggt ctgcctggtg ccgctgatcg
     1621 gcatcgtcgc cgtgcgcagc gcggcggctc ccgtcgcgat cgtcgcgatc gtgctgctcg
     1681 tggggcttct cgccgcggag ttcgtcgtgc gcttcttgat ctccgcgccg cttcagcggc
     1741 tgcgctggct cgccgcctgc atgctctcca tggccgtcgt ggcgctgatc gcgatgatgg
     1801 tctgcgtctc gatcgtgtgg tcttcggcgc tgcactgaac gcggcggagg ggtcagccga
     1861 gcggatgccg tcgggccgcg atccgggcga cggcgaacct gccacgcacg tcggcggatg
     1921 cggatgcgga tgccccgagc atcacggagt cgagtcgcgc cagcgacctt ccgtcgacca
     1981 gtgccgtgtc gccgagagcc acgaggatga cgattccgtc gcccgcttct gtgacgcccg
     2041 gatcgtcgac gcactgcacg tcgctctccc aggtgtcacg gcgggtcatc acgttgaggt
     2101 cgaggaagcc cgcctcgcgc gggctggcgg tcacctcggc ctcgccgtcg aagacgacga
     2161 tcccctcagg gtcaaggacc aggtcgcgcc cgtcgatgcg cagatccacc gcgccggggc
     2221 cgagtgcgac ctgacctcga cgcacgccgg ggtacgggct gtacggctgc gtgccggtga
     2281 tctccgcgat gctgacgcgc caggtccaca caccggcatc cgtcgcgtca tcgaacaggt
     2341 tccgcgtgac gccgcgcccg ttcttccacg gcgtcggcgg gatggcggag tactcgacga
     2401 tgaggggcgc gttcatgatg cgtccagtct cgcgcaaccg cgggtgccag actgtgccca
     2461 tgtccccgag ccgccgagtc ggaaagcgcc tgatcgtgat cgccgcgagc accgtcgcgg
     2521 ccctcggcat cctcgcggtc ctggtcttca acggagtgct ctggccggca cgcctgttcg
     2581 ccgcgtcata tcccgtgcgc gggatcgaca tctcgagcta tcagggcgac gtcgactggg
     2641 acgaactcgc gtcgcaggac atcgacttcg cgtacgtcaa ggcgacggag ggctcgaagt
     2701 cgaccgactc gcgtttctcc gcgaactggg cgggcgcctc tcgcaccgac ctgctgatcg
     2761 gcgcgtacca cttcgtgagc ttcgagagca ctggcgaagc gcaggccgcg cacatcatcg
     2821 agacggtccc gacgggagcc accctcccga tcgcactgga tctcgagtac tacggagact
     2881 tcttcgccca cccgccgacg cgcacgcagg tcgatgcgat cctggagccg ctgctgcggc
     2941 tgctggagga gcactacggc tcgccgcccg tcatctacgc cacgtcctcg gcgtacgacc
     3001 gctatctgtc cggcggctac gcggacaatc cgatctggat ccgatcgatc gtcctgcctc
     3061 cgacgctcga cgacggccgc gactggacga tctggcagta ctccgatcac gaccgcctcc
     3121 ggggctatgt cggtgtcgag cagtacatcg acatgaacgt cttccacggc acccgcgagg
     3181 agctggcggc gctcgcgaag tgagagggcg ccgctcccgc gcgtgtcagc gagtgatcac
     3241 gcgcggtgcc tcgtcctcga gtccgctgcg gatctccact ctcgtccacg gcacgggcgc
     3301 atcctcgaac acgggctcca gaacacgcca catcgcttcg ccctcgcctc ccacgaagta
     3361 gagctcgtag ctgtggtctc cgacctcgtt gccgtcgatc atccccatgc cggcctccgt
     3421 gagtgcgtcg tccgcggcga gctcggtctc cagcagcgca tccatgaccc ccaggtcgaa
     3481 ctcgaggtcg gtgtcgtcga agaccaccac gacccaggcg tctcccggcg gagccacctg
     3541 cgcctcgggg aacgcgggct ccgatgacga gcacgcagca acggacgcgg acgacagcag
     3601 catcaccacg ggcagtaccc aacgtcgaag gcgcatgcac agagactatg ggagaacggc
     3661 gtgtcccccg gtgagcgtgg cccgccgttc ccgcttccta agctgggagg gtgaacatct
     3721 gcgtcttcct ctccgcatcc gatctcgacg agcgctacac ccgtgctgct cagcaactcg
     3781 gacggctgat cggtgagcgg ggtcacaccc tggtgtgggg cgcgagcgat gtgggtctga
     3841 tgaaggtcct cgccgatgcg gtcgactccg cgggtggccg gctcgtgggc atctcggtcg
     3901 ggttcctcgc ggcgaaggca cggcagctgc agtcgccgaa cgagctgatc gtcggcgccg
     3961 acctcgccga acgcaaggcg acgctgctga cgcgcagcga tgccgtggtg atcatggtcg
     4021 gcggcagcgg cacgctcgac gaggcgaccg atgtgctcga actgcgcaag cacggcatct
     4081 acgacggccc ggtcgtcgtg ctgaacacgg acggcttcta cgacggcctg cagcagcagt
     4141 tcgcccgcat ggacgcggaa ggcttcctcc ccgttccgct cgctcagctg tgcaccttcg
     4201 tggatcagcc ggaggaggct ctcgcggtca tcgagcagca cgccccgaca aggcggtagg
     4261 cgactctctt cgcaccgctg cagcccagaa gttgcactag agtcggggcg agcgctggtg
     4321 cgcagaagga gcgatggcat ggagaacgtg accggcggcg aggcgtccac catcgacgag
     4381 cagaccttca tcgtcacccg gagcatctcg atagccgcac cgatcgagaa ggtctggcgc
     4441 gcgatcaccg agcccgagca catctcccgc tggttcgggc agaccgtctt cgacggcgcc
     4501 gggcgcggtg cacagggcac gatgacattc gacggccacg gcaccgtgcc gatccgcata
     4561 gagcagatcg acgcaccgcg cgccgtgacc taccgctggt gcaacgacaa cgccctcgac
     4621 tcgcctccga gcactctcga tgaggcgcat tcgacggtgt tcaccttcac cctcgaagtg
     4681 gaaggtgcgg gaacgcggtt gacggtcgtc gaagcgggct tcgagagcac ctccgccccg
     4741 ctgctgaaca tccagaggca tcgcgtcggc tgggacgagg aactcgacaa gctcatcgcc
     4801 ctgttcgagg ttccggcatg actcgcacgc tggttcccgt gctcgccgct ctgggcgatg
     4861 acacccggtg gagcatcctc gccgcgctcg gcgaaggcga cgcctctgcg tcggcactag
     4921 cgcagcgact cccggtgacc cgacaggcca tcgcaaagca cctggcaatc ctcagcgacg
     4981 tcggcgtcgt cgaacccgtg cgcgtcggcc gcgaagtccg gtaccgtgtc gtcggcgcga
     5041 cgctcagtgc cacggcgaac cgactcgacg cgatcggacg cgagtgggag cagcgcctgg
     5101 ccgccatcaa ggagatcgcc gaagacctct aggccgtggc cgattgatct gcaaccctgc
     5161 tattgcagaa gttcctccct agtgcaatcc tttagttgca caatatggag aggagcggca
     5221 catgctgctt gaggacaagg tggccgtgat ccacggtggg ggcggttcga tcggagctgc
     5281 gacggctcgc gccttcgcac gcgagggcgc gcggctgttt cttgcgggca gatcgctgcc
     5341 acgcatggag gcagctgccg aagaagccag agccgcgggc gcaaaggtcg aggtcgccgt
     5401 cgtcgacgcg tctgatcagg cggcggtcga ccaccacgcg gacgaggtgg cggagacggc
     5461 aggacgcatc gacatcgcgt tgaacgcggt cggcttcgac catgcacaag ggctggcgat
     5521 cgccgagacc tcgctggagg aatacctgta tccgatcatg gggtacctgc agacgaactt
     5581 cgtgacagcc aaggccgcgg cgcggcacat ggtcgcccag cgcagcggag tactcctgac
     5641 cgtctccacg cccggcgccc gcctgtcggg ccgggggctc atcggcaacg ccgcgcagtc
     5701 ggcgggactc gagggcttct cccgcgctct cgccggcgaa ctcggccccg acggtgtgcg
     5761 ggtggtctgc gtgcgcccgc acgccatctc cgacgcgctc acgacgtcgt acacccatga
     5821 gatgttcgga cgcatcgcgt ccgcgtcggg cctgccgatg gacgactggc tcgatggcct
     5881 cgccggcacg acggcgctcg gccggctgcc cgtgctcgac gaggtggccg agtacctcgc
     5941 attcgccgcc tccgatcgcg cagcctcgat gacaggcgtg atcgcgaacc tctcggcagg
     6001 agcactggtc gactgaacag aggcgagtgg cggtgccggg atcggcgcga tcccggcact
     6061 gccgggaata agaaaacccc cgcctgagcg ggggtttcat tcttgctggg gtacctggac
     6121 tcgaaccaag aacaactgaa ccagaatcag ccgtgttgcc aattacacca taccccaagg
     6181 gcgaaaccgg agcctcgcac cgagagtcaa gcttacctga ggcgctcgca gatggcgaat
     6241 cgacacgatc cacccgggcg tggcgagtct ccgccgtcag acggcgacca cctgcccgga
     6301 ctgcgccggc cggatccacg ggtccagatc gaagccctgg tcgcgcgcgg tgtcgagaaa
     6361 cgaccgctcc acggcatcct cgacctgcgg ttcgcgggag agcagccaca gatgcttccg
     6421 gtcaggcgtg ccgacgagcg cgtgccggta ctcgtcgtcc acccgcagca cccagtagtc
     6481 cgccttggtg aacgggatcc atcgcagcgc ctcgggtagg aacgtcacct gcaggcggcc
     6541 tggatgctca gcatcgggca ccgcctgccc cagagcccgc gtgggcttgc cgtcctcatc
     6601 acggcagcgg ttgtcgacgg tcaccgtccc gtcttcggcc atcgtgtact ccgcggtcac
     6661 atcggtggcg cggtcgtcct cgaagcgcag cggcagccgg ccgacctcga accacaggcc
     6721 cagataccgc cgcagctcca gtgcgggcac ggaacgcacg tcatccgtca tgatgctcct
     6781 cctctgctcc cctgtcacac tacggatcgc atgccggcga cagaacgggg ttgcacctct
     6841 cgcggctggc gactacaggg ccctcagagc tcgcccatcc gcgcgggctt cgcccagcgc
     6901 aggaacaccg tggaccgcgt gatccgcgcg agatccgcga gcagggcgcc gaccttggtg
     6961 ccgatgcaca ggctcaggaa catcatcggc gcggacacga gtgcctgcgc gtcgaagctg
     7021 gccagcgaga ccaggccgac ggtgcccggg acgagcagca ggaacgccgg ctggaaggac
     7081 accgtggccg ggatcgcgat cgtcagacgc tccagcactc gcgcggccac gaacagcacc
     7141 gccgccgcga tgccggtcgc gacgacgctg ccgaccacgg ggctgaacag tgccagcacc
     7201 gcataggtcc ccgccatcac gaccacgcac acgacggtga ggcggaatcc cgatccgaac
     7261 gcgagcccga tgccgatcgc cagcacgatc accgccagcc atgcggacca cagcggcggc
     7321 agcgcctccc atcccccgcg gtcggtggtc agctccacgg cctcccccac cagagcggcg
     7381 gatgccgagt caacgcgcag accggtgagc agcgctcccg aatagacacc cgcagccatg
     7441 aacgcgagca tgatcagccc gtacatcagg cgggacgcac ccgtgacgat gtcggtggag
     7501 gtcagctcga gcagagcgtt cgtgatcagt gcgccgggta cgaggatcgc caccggcgcg
     7561 cacaccgcga acagcggcac cggcccgaga tccagccagc ccgcgacagt gcccaccagg
     7621 atcgtcgaga cgaacgccga gacgaagggg gcgacggccg ccgcggcccg aacgcgcatc
     7681 atcagcatcg tgagaccgcc gaccagcagc cccacgagca gtgccagcac gatcgcccac
     7741 caggggcagc ggaacagcca tgccagagac agcgacagca gcccgcatcc gatcacgctc
     7801 tgcagcgcgg gacgatgcct cggcgtcgct cggatggcgg ccatccgggc cggggcctcg
     7861 gccagtggca cagcacccga ctcgagcccg tgcaccagcc tgctggcctt cgccgactga
     7921 cggaacgtca gcgcctcgcc cttgccgatg acggtggtcg tcgccgagga cgcgccggga
     7981 cggctcacca tcaccatctc cggcaggatc gcgaactcga gggacgcctg cggagtcgac
     8041 gccgcgcgcc gcccgacctg ctcgagcgag ccccgcacat cggtcacgga gacgccgcac
     8101 tccagcagca gaacgccgag gtcgccgagg atgacatccg tctcgactgc cggaggcgcg
     8161 gcgtccgtca ccggagtcag cccagggtgt cggcgagtgc ccgcaggcga cgcagcgtct
     8221 cgtccttgcc gagcagctcc atcgactcga acagcggcgg ggacacgcga cgaccggtca
     8281 gggcgacgcg cggcggcccg taggcgacgc gaggcttcag accgagctcg tcgacgagct
     8341 tcgccgagag cgccgtctgc acggcatccg gggtgaactc ctccaccggc tcgagcgcgg
     8401 cgacgcacgc ggcgagaacc tcggcggcgt tcgcgggcag gcccttcatg gcgtccgcct
     8461 cgtagctcac ctcgtcggtg aacaggaagc ccagcatgcc gggcgcctcg cccagcagct
     8521 gcatgcgctc ctgcacgagc ggggcggcac ggaacgccag gacgatctgc tcgtgcgtgg
     8581 gctcggccgc gccgaacacg cccgcatccg ccaggtaggg cagcatccgc tcggcgaagt
     8641 ccttgccgtc gagctgacgg atgtggtcgc cgttgatcga ctcggccttc ttctggtcga
     8701 agcgagccgg gttcgggttg acgtcgacga tgtcgaacgc ctgaatgaac tcctccagcg
     8761 agaacacgtc gcggtcggga ccgatcgacc agcccagcag cgccaggtag ttcagcaggc
     8821 cctcgtggat gaagccgcgc tcgcggtgca ggaacaggtc ggcctgcggg tcgcgcttgg
     8881 acagcttctt gttgcccgtc tcgccgagca ccagcggcat gtgcgcgaag cgcggcacga
     8941 agtcggtgac gcccgccttg gtcagcgcgt cgtacagcac cagctggcgg gccgtggagg
     9001 gcatgaggtc ctcgccgcgc agcacgtggg tgatgcccat cagcgcgtcg tccaccgggt
     9061 tcacgaacgt gtacagcggc acgccgttcg ggcgcacgat cacgtagtcc gggaacgaac
     9121 cagcggggaa ggtgacctcg ccgcggatca ggtcgacgta ggtgaggtcc tcatccggga
     9181 cgcgcaggcg cagcgcgggc tggcgcccct cggcgcggaa ggacgccttc tgttcctcgg
     9241 tgaggtcgcg gtcgaagttg tcgtagccga gctgcttggc ccggccgttg gcctcgttgc
     9301 gcgcgtcgat ctcctcggcg gtggagaagc tctcatacac ggcgccggac gccttcagct
     9361 tctcgatcac ctcacggtag atgtcgtgac gctgcgactg ccggtacggc gcgtgcgggc
     9421 cgcccacctc gacgccctca tcccagtcga tcttcatcca ggtgagcgcg tcgaccagct
     9481 gccggaagct ctcctcgctg tcgcgggcgg cgtcggtgtc ctcgatgcgg aagatgagct
     9541 tgccgccgtt gtgccgcgcg taagcccagt tgaacagggc tgtgcgcacc atgccgacgt
     9601 gcggcaggcc ggtgggcgaa gggcagaagc gcacgcggat gtcggatccg gtggcggtgg
     9661 tggtgagggg gtgcacagta gccatatccc ctcgattcta tcgggggcgg atgccgccgc
     9721 cgagacggct caggcggtcg cggccgggtc ggcctcgacc gccgtctcgc ggatccgtcc
     9781 cagcggcgag agcgccgtga tcgcgatcac gaacaccagc gccacgatcc cgagcccggc
     9841 gtagtcgaag gtcgtgagga cccagcctgc agcggctgcg ctcgcagccg ccgtgaggct
     9901 catcagcgaa tcgctgatgc cctgacggcg gggcctcatc gtgacggtcg acgactccgt
     9961 gagcagtgcg gccccggcga cggtcgcggc gctccagccg agtccgagca ggatcagggc
    10021 gatcatgacc ccggcgggcg tgcggccggc gaagatcgcc gtcagcagcg atgcggccag
    10081 gacggcctgc ccgagcagga ccacgcgcac gcgaccccag cggtccgaga ggatgccgaa
    10141 cagaggcgag agcccgtaca tgcctgccac gtgcagcgcg atcgtgatgc ccaccagcag
    10201 cgtgacatcc gtgctcgacg gcatgctgcc gtgcgccccg tgcgccatgt gcgataggtg
    10261 gatgggcgtc atcgccatca cagaggccat cacggcgtgc gaaccagcaa cggcgaacat
    10321 cgcgtaccgg gcgacgaccg gacggtcgac gccggttccg agcggcgagg cggtcgtggt
    10381 cgtcttcgcc aggtgcagcg cggtcagcag cgggtccggt cgcagggcga ccaggtacag
    10441 gatcagggcg gcgatctgcg cgaccagcga gaacaggtac gagcccgtca gcggcggcat
    10501 cccgatcgca tggccgagag cctcgcccgg cgtgagcagc agcggaccgg tgacgccgcc
    10561 gacggtcgtc gaccacacca cgacggagag gtcccgcgcg cggtgggcgg gcgcagccag
    10621 gtcggtcgcg gcgaaccgcg actgcaggtt gcccgcgttg cccatgccga tcatgatgat
    10681 cccgatcagc agcagcggga acagacgggt cgcggccgcg gtgatcaccg tggccacccc
    10741 gatcagggcc agcaggttgc ccgcggtcag cgagagccgg cgtccacggc gggccgcgaa
    10801 ccgggccagc gggatcgcga aggccgcggc cccgagggtg acggatgccg tcgccagtcc
    10861 cgacagcgcg tcgctgccgg agagatccgc agccagcagg gcaccgaggg acacggtggc
    10921 cccgaaggcg atgcccccga ggacctggcc gagggagagg atgcggacgg tgcggcgctg
    10981 gatcgccgcg agttcgtcga gggtgagcgc ggcgctcacg agacggggac cgcgtcgcgc
    11041 accgggttgc gcagggtgcc gatgccggag acctcgacct cgatcgtgtc gcccgcgacg
    11101 accggtccga cgcccgcggg ggtgcccgtc atgatcacgt cacccgggag cagcgtgaac
    11161 gccgccgagg cgtaggcgat gatgtcggcc acggagtgga tcatgtcggt cagcggcgcc
    11221 tgctggcgca cctcgccgtt gacgcgcgtc tcgatgcggg cggatgccgc atcgaactcg
    11281 gtctcgatca ccgggcccag cgggcagaac gtgtcgaagc ccttaccccg tgtccactgc
    11341 ccgtccttgc gctggatgtc gcgtgcggtg aagtcgttgc ccacggtgta cccgaacacg
    11401 taatcgagag cgtcctcggc cttgatgttc ttggcgatgc gcccgatcac cacgaccagc
    11461 tcgccctcga actccgtgcg ctccgagatc gccgggcgca ggatggcgtc gcccggcccg
    11521 atcaccgatg tgttcggctt caggaacagc agcggctcct cgggtgcgac gttgccgaac
    11581 tcggcggcgt gctcgtggta attgcgtccc acgcacacga ccttggagcg cgggatcacg
    11641 ggcgccagca gtgcggcatc cgcgagcggc acccgctccc ccgtcgtgtc gaatccggcg
    11701 aacatggggt cgcccgcgag cacgaccagg tcgccctcgt cgacgatgcc gaaccggatg
    11761 ccgtcactgt ggctgaaacg tgcgatcttc acccggccag cctagccgcc accgggcctc
    11821 agccgtcgag ccggagcagc cagccgtgct tgtcctcgcg acgcccgtac tggatgtcgg
    11881 tgagctcctc gcgcagcgac agcgccaggt cgcccagggg ctgcgcgtcc tcgaagccgt
    11941 cggcgaccag cgcgccgatc ggggtgacca cggcggccgt gccgcacgcg aacacctcga
    12001 cgatgtcgcc ggatgccacg ccctgacgcc actcgtccag cgagaccgga cggcgctcga
    12061 cggtcagacc gcggtcctcg gcgagctgca gcagcgagtc gcgcgtgatg ccctcgagga
    12121 tggagtcgga ttccggggtg acgacgcggc cgtccttgta cacgaagacc acgttcatgc
    12181 cgccgagctc ctcgacgttg ttgtcctggt cgaggaacac gacctggtcg cagcccttct
    12241 cgtacgcctc tgcctgcggg agcaggctgg aggcgtagtt gccgccggtc ttggcggctc
    12301 ccgtgccgcc cttgcccgcg cgggcgtagt tctcgctcag ccagatgcgc accggcttca
    12361 cgccgccgga gaagtaggcg ccggcgggcg aggcgatcag gtagtaggcc accttcttcg
    12421 ccgggcgcac gccgaggaac gcctccttgg cgaacatgaa cggccgcagg tacaggctct
    12481 ggtcagcgcc ggagggcacc caggccccgt cgaccgcgac cagctcgcgg atcgactgga
    12541 tgaaatgctc ggtgggcagc tcgggcagcg ccagtcggcg ggcactgcgc tgcaggcggg
    12601 ccgcgttgcg gtcgggccgg aaggtgtgca ctgaaccgtc ggcgtggcgg taggccttga
    12661 tgccctcgaa gatctcctgg ccgtagtgca gcaccgcggc ggccgggtcg agagagatcg
    12721 ggccgtaggg ctgcacgcgc ggacggtgcc agccgccgtt ctcggaccag cagatgtcga
    12781 ccatgtggtc ggtgaagtgc aggccgaatc cggggttctg caggatctcc tcacgctgag
    12841 ccgggctctt ggcggcgagg ttcttcgtga cggcgaaggt cagaggtgcg gtggtggtgt
    12901 cggtcatgtc tgtcctcgat cgaatgcggc ggcgatgcgc acgtactgtc agattacgcc
    12961 tgcagcaggg cgatcacggc gtcgccggtc tcgctggtgc tgcggctgcc cgaacgggct
    13021 gcgatgtcct gctcgacggc gcgctgcacg cgcgcggcct cgtcgcgcag cccgagatga
    13081 tcgagcagca gggcgacgga caggacggcg gccgtggggt cggccttctg ctgtcccgcg
    13141 atgtcgggcg ccgaaccgtg cacgggctcg aacatcgagg ggaaggcgcc gtccgggttg
    13201 atgttcccgg aggccgccag gccgatgccg cccgtgacgg cgccggcgag gtcggtgagg
    13261 atgtcgccga agaggttgtc ggtgacgatc acgtcgaagc gcgcgggatc ggtcaccagg
    13321 aagatcgtgg cggcgtcgac gtgcagatag tcgaccgtca cgtcggggtg ctcggttgcc
    13381 acctggtcga cgatccgctt ccagatcgcc ccggcgtgca cgagcacgtt ggtcttgtgc
    13441 acgagcgtga gcttcctgcg gcgacgctcg gcgaggtcga aggcgtagcg gacgacgcgc
    13501 tccacaccga acgcggtgtt cacgctggtc tcgttggcca cctcgtgcgg tgttcccttg
    13561 cggatgctgc cgccgttgcc gacgtacggg ccctcggtgc cctctcggac gaccacgaag
    13621 tcgatctcgc caggatccgc gagcggtccg ggtgcgccgg cgaacagctt cgagggacgt
    13681 aggttcacgt agtggtcgag ctcgaagcgc agcttcagca gcagtccgcg ctcgatgttg
    13741 gcatccttca gtcgcggatc gcccggcact ccgccgacgg cgcccagcag gatggcgtcg
    13801 tgcgaacgga tcgcgtcgag gtccgcatcc gtcagcgtgt ccccggtcgc gaggaagcgg
    13861 tcggcgccga gcgagaacgg cgtcttctcg aacgcgacgt cgctgcccgc cgtgacggcg
    13921 tcgagcacgc gctcggcctg ggcgatgacc tcacggccga tcccgtctcc ggggatgacg
    13981 gccagcttca cgacgcgcga catgggactc ctctgcacca cggcccgccg cgctggtcgc
    14041 cgcggacact gcctcccagc gtagcggcgc acgcacgccg ctcccgactc cgagtcaccg
    14101 tcgggagcgg gcggatgccg ctcagccggc cgagccgttc cgggacggct tcagcgtcgt
    14161 gagcgcgacg acgatcgcgc cgatcacgag gagcgctccg atgacggagg tcgtgacgac
    14221 gcccgagccg aaggctgccg tcgcggcgtc ccacagcgcc gagccgagcc ggccgggcag
    14281 ctcctgtgcg gcggtgaaag cgccggcgag tgtctccctg gcatgcgcgg ccagctccga
    14341 ggggatcccg tccgggatca cgagcgcacc gcggtagaac gcggtgatga tgccgccgag
    14401 cacagccgtg ccgagcaccg cgcccagctc gtacgccgtc tcagagaccg cactcgcagc
    14461 cccggccttc tcagccggtg catgagcgag gatcagctcg ttagagatgg tctcggccgc
    14521 gccgatgccg acaccgagca ccatgaaggc gatcagcagc cagccgacgc tgttcagtcc
    14581 cgcggtggcg gcgacgatca cgtagccgag cgccgacagc gacaggccga cgggaaccac
    14641 gatgtgcggc gcgacccgcc gcgagatggg cacgacgccc agacccgcga tgatcatcgc
    14701 caccatcccg ggcacgagag cgagacccgc gaccatgggc gagagcccga tcaccagctg
    14761 cagctgctgc gacacgaagt agaggaatcc gaccagcgcg atcacgctca gcaggttcac
    14821 gaggatcgca ccgctgaacc cgccgcgacg gaacagcgtc atgtcgagca tcgggttctc
    14881 cgcgcgccgc atccggcgga cgaagaggaa tccggcgaac accccgacga gtaggaagat
    14941 cgcggcggcg aggttggggc cgtccacggc gaaggacttg atcgcgaaga ccaccgggat
    15001 catcgccgcc atcgacagca cgatgccgat cgggtcgatc cgcccggggt tcgggtcgct
    15061 gctctcgggc agcagccaca ggccgccgat cagcagcggg atgagcacgg gcacggcgat
    15121 gaggaacacc gagccccagc cgaagtgctc gagcaggaag ccgccgacga ccggcccgag
    15181 cgcggagccc gccgagaacg ccgacgccca caccgcgatg gcgaggcgcc gctgatcgcg
    15241 gttgcggaag atggagcgca gcagcgacag ggtcgagggc atgagcatcg cgccgaacac
    15301 gccgagcgcg gcccgcgcgg cgatgagcag cccggccgag ggggcgaagg ccgcgagagc
    15361 ggagacggca gcgaagccgg tggcgccgat cagcagcatc cgccggcgtc cgaaccggtc
    15421 gccgagcacg cccatcgtga cgagcaggcc cgcgagcacg agcgagtaga cgtcgatgat
    15481 ccagagctgc tcggcgccgc tgggctgcag agacaccgag atctcgggca gcgcgaacgc
    15541 gagcaccgtg ttgtcgaccg acaccagcag caccgggagc atgagcaccg cgagcgcggc
    15601 ccagccgcgc agaccgacgc ggggtccgtc gactccggtg agctcgatgg gtgcagtggc
    15661 gggcatggtg tcctccgata acttaaccgt ccagctggta cagtaacaga tgtcggcctg
    15721 atcggatgct gtgcagccgg cgcccgcgaa gagcaaggag tccccgatgt cccgccctcc
    15781 cctggcgcgc gaacgcgtgc tcgacgccta cgagtcgatc ctcatcgccg acggcgagcg
    15841 tgccgcgacg ctcgacgcca cagctcaggc cgcaggcgtc tccaagggcg gtctgctcta
    15901 tcacttcgcc tcgaaggacg acctggccgc agggctcgcc gagcgcctcg ccgtgctcac
    15961 ggaccaggac ctggagcgca tgcgggaagc accggacggc gccgtcgcct actacatccg
    16021 cacctcggtg atggaggacg acgccctgga tcgggcgctc atcgcgacca cgcggctggc
    16081 tcagggcggc tccgccgccg catccgccgc gctgcgggag gtgcgccgcc gatgggcgga
    16141 tgcgatccgg ccgcatgtgc gcgacgagac cgctctggat ctggtgatgc tgctgagcga
    16201 cggcctgtac ttcaacaact cgctctgcga gcccgtggac ggccacgtct cggcggacga
    16261 cgggttcgtg ccgaccggcg cagcactggc gcggctcgtc gagctcgtcc gcagctcgac
    16321 ggcctgacct ccctagtcgc gcgcggcgag cagccgacgc tcgaggtggc ggcgcaccgc
    16381 cgagtcctcg gtgaggccga tcgcctcgcg caga
//
LOCUS       DATMSX010000453         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_177609,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000453 DATMSX010000000
VERSION     DATMSX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177609"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2871)
                     /locus_tag="VN041_14555"
     CDS             complement(<1..2871)
                     /locus_tag="VN041_14555"
                     /inference="COORDINATES: protein motif:HMM:NF025943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HWU30301.1"
                     /translation="MSYAHLSLRRRALTALTAAALVVPLGLIAQSGAAHAAATSGEQE
                     ASVLISQGRPTATSSIEDAGFEGAKAVDADVTSTRWASEEGVDPQWIRVDLGAGATVS
                     KVVLKWEAAYASRYRVEISADGTSWTTLATESAGDGGTDEFTALTGTGRYLRVYGTAR
                     GTGYGYSLYDLKVYGTTGTGGTDPSTGDVISVSTAAQLTIALAAVKPGQTIKLAAGTY
                     RGSFVAETAGTASAPITLTGPRDAVIINEASSGTLSGCPAPSADWNSGYGLWLFGASY
                     WNVTGLTVADSKKGIVLDGATHVTIDGVLVRDIAEEGVHFRRSSADGVLRNSEIVRTG
                     LVKPGYGEAVYLGSANSNYDCYADASGFDRSDRIQVLDNRIGPGVAAELIDVKEGTRD
                     GIVRGNTFNGTGLSGENSADSWADVKGNGYLFEGNTGTFASPGVFANGYETHNPLTGS
                     GCGNIWRGNSSDLGGVGQYAVNVTSTSKCADAPNVVMDTNTVANAVTGLTNIAVTPSG
                     TIPAEPGSAATVVSSISALQSAIDSADAGDTIVLKNGSYSVSGSIDVSGGGTAGVPVT
                     IKAETVGGVTLSGSKSFTFSGAHDVVIQGFRFTQSTTLEVPADSRRITFSRDEFVFAQ
                     SAEHNLMIRADDSVVEYSWFHGKNTIGVYLGIEGAGTTDMATGVRVHHNYFSDQNFTG
                     SNGGECIRLGVSPRALSVAGAVIEYNLFENANGDPEAISVKSSGNTVRYNTIRNSFGG
                     IVLRHGNDNTVMGNRIMDGTNGIRIYGNDHEIIDNYLAGLSKDGIVIGSGTERDHYEG
                     EPAESRKLNDAPDRIRIALNTIVGNGDGISGETNRTVPPLNVTIVDNIVQGDTGHLAD
                     VPLMQDFYWRGNILWGAAVNGNIPKVGFVRVNPQLVKDAAGTWRIGASSPAVDAATQT
                     NHANWVADDIDGRARTGAYDVGAHEVTTAPATRAPLTTADVRS"
     gene            complement(3031..3783)
                     /locus_tag="VN041_14560"
     CDS             complement(3031..3783)
                     /locus_tag="VN041_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010549301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWU30302.1"
                     /translation="MTMRPGLCSVTFRQLAPEEIVALAAEAQLEVIEWGGDVHVPPGD
                     QDRAADVSRVTRDAGLDVCSYGSYFRAGAYETLTPILDSAEALGADRVRIWAGRVDSA
                     DATPEQYAQIVARLRDAAAEASERGIGLALEYHRGTIADNPDAVLRLLADVPALSTYW
                     QPSVDAPEATALAEFDAIAAHTSAVHVFSWWPFNERLRLHERQGMWQALCSAASALPV
                     PPRDALLEFVPGDDPALLAPEAATLRGWLARA"
     gene            complement(3819..>4388)
                     /locus_tag="VN041_14565"
     CDS             complement(3819..>4388)
                     /locus_tag="VN041_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWU30303.1"
                     /translation="AAGSTAVLVLVAGGLPAGSRDLVGARERVRDALGELAPYAQQAG
                     VTLAIEPLHPMYASDRCVVSTLGQALDIAADFAPEVVGAAVDTFHIWWDPQVLDQIAR
                     AGHEGRIATYQVCDWKTPLAEDVLLSRHYTGDGVIDFGSLTRAVMATGYDRDIEVEIF
                     NADIWADAPEDVVRRTAETFSAAVSPHLA"
BASE COUNT          640 a         1500 c         1520 g          728 t
ORIGIN      
        1 cgatctcacg tcggccgtgg tgagcggggc acgggtcgcc ggtgcggtgg tgacctcgtg
       61 cgctcccacg tcgtacgcac cggtgcgggc gcggccgtcg atgtcgtcgg ccacccagtt
      121 cgcgtggttc gtctgggtcg ccgcgtcgac ggcggggctg gacgccccga tccgccaggt
      181 gccggcggca tccttcacga gctgcgggtt cacccggacg aagccgacct tcgggatgtt
      241 gccgttcacg gccgcgcccc acaggatgtt gccgcgccag tagaagtcct gcatgagcgg
      301 gacgtcggcg aggtgccccg tgtcgccctg gacgatgttg tcgacgatgg tgacattcag
      361 cggcggaacc gtgcggttcg tctcgccgct gatgccgtca ccgttgccga cgatcgtgtt
      421 cagtgcgatg cggatgcggt cgggggcatc gttcagcttg cgcgactcgg cgggctcgcc
      481 ctcgtagtga tcccgttccg tgcccgagcc gatcacgatg ccgtccttgc tgagacccgc
      541 gaggtagttg tcgatgatct cgtggtcgtt gccgtagatc cggatgccgt tggtgccgtc
      601 catgatcctg ttgcccatga cggtgttgtc gttgccgtgc cgcagcacga tgccgccgaa
      661 gctgttgcgg atcgtgttgt agcgcaccgt gttgccgctg gacttgaccg agatcgcctc
      721 cgggtcgccg ttggcgttct cgaacaggtt gtactcgatg acggctcccg cgacactcag
      781 cgcgcgagga ctgacaccga ggcggatgca ctccccgccg ttcgaaccgg tgaagttctg
      841 gtcggagaag tagttgtggt gcacccgcac accggtggcc atgtccgtgg tgcccgcacc
      901 ctcgatgccg agatacacgc cgatggtgtt cttgccgtgg aaccagctgt actccacgac
      961 ggagtcatcg gcacggatca tcaggttgtg ctcggcgctc tgcgcgaaga cgaactcgtc
     1021 tcggctgaac gtgatgcgcc tgctgtccgc cggcacctcc agggtggtcg actgggtgaa
     1081 ccggaagccc tggatgacga cgtcgtgcgc gcccgagaac gtgaagctct tcgagccgct
     1141 cagcgtcacg ccgccgacgg tctctgcctt gatcgtcacg ggcacccccg cggtgcctcc
     1201 tccggagacg tcgatgcttc cggagacgct gtaggagccg ttcttcagca cgatggtgtc
     1261 gccggcatcc gcggagtcga tcgccgactg cagcgcggag atcgagctga ccacggtcgc
     1321 ggcgctcccg ggctcggcgg ggatcgtccc cgacggcgtg acggcgatgt tcgtgagacc
     1381 tgtgacggcg ttcgcgaccg tgttcgtgtc catcacgacg ttgggggcat ccgcgcactt
     1441 ggacgtggag gtgacgttca cggcgtactg ccccacacct ccgaggtcgg agctgttgcc
     1501 gcgccagatg ttgccgcaac cggatccggt cagcgggttg tgcgtctcgt agccgttcgc
     1561 gaagacaccc ggcgaggcga aggtgccggt gttgccctcg aagaggtagc cgttgccctt
     1621 cacgtccgcc caggagtcgg cggagttctc gccggacagc ccggtgccgt tgaaggtgtt
     1681 gccgcgcacg atgccgtccc gcgttccctc cttgacgtcg atcagctcgg ccgcgacgcc
     1741 ggggccgatc ctgttgtcga gcacctggat gcggtcggag cggtcgaagc cgctcgcgtc
     1801 ggcgtagcag tcgtagttgg agttcgccga accgaggtag accgcttcgc cgtatcccgg
     1861 cttcaccagt ccggtgcgca cgatctcgga gttgcgcagc accccgtctg cggacgagcg
     1921 gcggaagtgc acgccctcct cggcgatgtc ccgcaccagc actccgtcga tggtgacgtg
     1981 ggtggcgccg tcgagcacga tgcccttctt cgagtcggcg accgtcagcc cggtgacgtt
     2041 ccagtaggag gctccgaaca gccacagccc gtatccggag ttccagtcgg cggacggcgc
     2101 cgggcagccc gacagggttc ccgaggaggc ctcgttgatg atcacggcgt cgcgaggacc
     2161 ggtcagggtg atcggcgcgg atgccgtgcc cgcggtctcg gcgacgaagc tgccgcggta
     2221 cgtgccggcc gccagcttga tggtctgccc gggcttcacg gctgcgaggg cgatggtcag
     2281 ctgggcggcg gtggagacgc tgatgacatc ccctgtcgag gggtcagtgc cgccggtgcc
     2341 ggtggtgccg tacaccttga ggtcgtagag cgagtatccg tagccggtgc cacgggcggt
     2401 gccgtagacg cgcagatagc ggcccgtgcc ggtcagggcg gtgaactcgt ccgtgccgcc
     2461 gtcgccggcg gactcggtcg cgagggtcgt ccacgacgtg ccgtccgcgg agatctcgac
     2521 gcggtacctg ctcgcgtacg cggcctccca cttcagcacc accttcgaca ccgtcgcgcc
     2581 cgcgcccagg tccacgcgga tccactgcgg atccacaccc tcctcactgg cccaccgcgt
     2641 cgacgtcaca tccgcatcga ctgccttcgc cccctcgaaa cccgcgtcct cgatcgacga
     2701 cgtcgccgtc gggcgaccct gcgagatcag gactgaggcc tcctgctctc cgctcgtggc
     2761 agccgcatga gcggctccgg actgcgctat gagccccagg gggacgacga gcgcggcggc
     2821 tgtcagagca gtcagcgccc ggcgccggag ggagaggtgg gcataggaca tcgtcgtctc
     2881 ctgcacggtt cgaagcggtt cacgatcagc ccggccggct gttcgtaaaa cttcttacca
     2941 taagggatcg acccagcacc agactttttg gcaaacgctt gccagattcc tcgagacagc
     3001 ccccgcctcc aggcgtcgac cccgcggtcg tcaggcgcgg gcgagccagc cccgcagcgt
     3061 cgcggcttcg ggagcgagca gcgccggatc gtcgccgggg acgaactcga gcagggcgtc
     3121 gcggggcggg acgggcagag cggatgctgc ggagcacagc gcctgccaca tcccctgtcg
     3181 ctcgtgcagc cgcaggcgct cgttgaacgg ccaccacgag aacacgtgca ccgcactggt
     3241 gtgcgcggcg atcgcgtcga actcggcgag cgccgtcgcc tccggggcat ccaccgacgg
     3301 ctgccagtag gtggacagcg ccggcacatc ggcgagcagg cgcagcacgg catccgggtt
     3361 gtcggcgatc gtgccgcggt ggtactccag cgcgaggccg atcccccgct cggacgcctc
     3421 ggccgcggca tccctcagcc gcgcgacgat ctgcgcgtac tgctcgggcg tcgcgtcggc
     3481 ggagtccacg cgcccggccc agatccgcac ccgatccgca ccgagcgcct cggcactgtc
     3541 gaggatcggg gtgagcgtct cgtaggcacc ggcacggaag tacgagccgt acgagcagac
     3601 gtcgagccct gcgtcccgcg tcacccgcga gacgtcggcg gcgcggtcct ggtcacccgg
     3661 gggcacgtgc acgtcgccgc cccactcgat cacctcgagc tgcgcctccg cggcgagcgc
     3721 gacgatctcc tccggggcga gctgccggaa ggtcaccgag cagagtcctg ggcgcatggt
     3781 catgtcttcc atcttcgtcc agtcgtcctt cgtgcgcgtc aggccaggtg cggggagacc
     3841 gccgcgctga aggtctcggc ggtacgacgc acgacatcct ccggcgcatc ggcccagatg
     3901 tccgcgttga agatctccac ctcgatgtcg cgatcgtagc cggtcgccat caccgcacgg
     3961 gtgagcgagc cgaagtcgat cacgccgtcg cccgtgtagt gccgcgacag cagaacatcc
     4021 tcggcgagcg gggtcttcca gtcgcacacc tgataggtgg cgatgcggcc ctcgtgaccg
     4081 gcacgcgcga tctggtcgag cacctgcgga tcccaccaga tgtggaacgt gtcgaccgcc
     4141 gcaccgacca cctcgggggc gaagtccgcc gcgatgtcga gagcctgtcc gagcgtcgag
     4201 acgacgcatc ggtcggaggc gtacatcgga tgcagcggct cgatcgccag cgtcacgccg
     4261 gcctgctggg catacggagc cagctcgccg agtgcgtcgc gcacccgctc gcgggcgccg
     4321 acgagatcac gggagcccgc gggaagaccc ccggcgacga gcacgagcac cgcggtggaa
     4381 ccagcggc
//
LOCUS       DATMSX010000454         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_64492,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000454 DATMSX010000000
VERSION     DATMSX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64492"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VN041_14570"
     CDS             <1..671
                     /locus_tag="VN041_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HWU30304.1"
                     /translation="ALLDALLRFRAGTASQAEVVDAFRPARVLVPLVAEKGDEGIGPT
                     GLKVDKTQELSIVTVAAPDGRRVQPAFSSVDTMRRWDAQARPIPVEAARVALSAAGEE
                     TDLIVLDPASDTEFVIRRPAVWAIAQGQPWEPSFLSPEVFSALRESIAHELAVIDVSV
                     ASGDPDARMRGPELIVTLELIDGLDRETLDAVLARLAQRWAADDRMAVLVDSLTVKLT
                     RSAG"
     gene            complement(827..1213)
                     /locus_tag="VN041_14575"
     CDS             complement(827..1213)
                     /locus_tag="VN041_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HWU30305.1"
                     /translation="MTIPAEHADDRHERWAEQEQAAAAATRDIADVPAVEVITTTAVH
                     LMSAAAVKLGLADDPDAQLDLDEARKLINALAGLITAGAPEISSAHASPLRDGLRSLQ
                     LAFREASPIQDPIGKGPGEKWTGPVT"
     gene            1426..2082
                     /gene="infC"
                     /locus_tag="VN041_14580"
     CDS             1426..2082
                     /gene="infC"
                     /locus_tag="VN041_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWU30306.1"
                     /translation="MAETTSKELRISDPRTNERIRVPEVRLVGPAGEQIGVVRIEAAL
                     RLAQEADLDLVEVAPNSKPPVVKIMDYGKFKYEAAQKAKEARRNQANTILKEVRFRLK
                     IEAHDYTTKLKRAEGFLKAGDKVKAMILFRGREQSRPEQGVRLLRKFAEDVAEFGTVE
                     SNPTIDGRNMVMVVAPLKNKSEAKAEQNAVRTANKQAAREAKSSTGAASAESTDETAA
                     "
     gene            2156..2353
                     /gene="rpmI"
                     /locus_tag="VN041_14585"
     CDS             2156..2353
                     /gene="rpmI"
                     /locus_tag="VN041_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWU30307.1"
                     /translation="MPKQKTHSGAKKRFKITGSGKLKKQQAGMRHNLEHKSSRQTRRL
                     NQDQVLSKADTKVANKLLGRG"
     gene            2386..2772
                     /gene="rplT"
                     /locus_tag="VN041_14590"
     CDS             2386..2772
                     /gene="rplT"
                     /locus_tag="VN041_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWU30308.1"
                     /translation="MARVKRAVNAHKKRRVILERASGYRGQRSRLYRKAKEQVTHSLV
                     YAYRDRRKRKGDFRRLWIQRINAAARQNGITYNRFMQGLGLAGVQVDRRMLAELAVTD
                     AAAFATLVDVAKKALPSDVNAPKAAA"
     gene            2852..3658
                     /locus_tag="VN041_14595"
     CDS             2852..3658
                     /locus_tag="VN041_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWU30309.1"
                     /translation="MLENPRSPRVRAVAKLTKRSARTETGLFLLEGPQSVREALNYLP
                     GAIVELFSTPSAWEKHSDVRALASEHGIDVEYVTEQVLAAMADTVTPQGIVAVARQTP
                     TSVREIFAAGPRLIAICEEVRDPGNLGTIIRAADAAGADAVVLTGRTVDPYNPKVVRS
                     TTGSLFHLPFSVGGDLNDVVSRAHDAGLQVLAADVKGDDLLAARAEGVLERPTAWLFG
                     NEARGLEDDMLDLADRALKLPIYGRAESLNLATAASVCLYESAFAQRVAG"
     gene            3663..4340
                     /locus_tag="VN041_14600"
     CDS             3663..4340
                     /locus_tag="VN041_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30310.1"
                     /translation="MRILIVEDDDRVAEALQAFLNRSGYATVRAVDGAQALGLLGSDT
                     EVVLLDLGLPDMDGIDVCRRIRAQSGVPVIIATARSDVQERIRGLRAGADDFVVKPYD
                     VRELVARIEAVTRRLHPLDSHHPASGDRSLIEVGDVSIDLVARRVLVDGAPVELTRKE
                     FDIIAVLARYPGVAVPRDRLIREVWNTDWQSFARSLEVHVASIRRKIGRASLIETVRG
                     VGYRLEG"
     gene            4344..>5319
                     /locus_tag="VN041_14605"
     CDS             4344..>5319
                     /locus_tag="VN041_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWU30311.1"
                     /translation="MRRRLIVVFLVPLVLVLMVLGGAYAWSAARSVQQEFYAEQLGDL
                     SYFTTTARQALLSGSAEVFDGEAQRYRELYGTRVVIVDRAGRPWAPSTAQPVFDSPEI
                     EAQIKLALSGRRGELPQNALPWQFSDLSLVEPVFDGGDVIGAVVMSASVDVPRAEIGR
                     QWLLITAVATLVIAVGLLVVFRLAGWVLRPVRRLDRAMEAMERGDMEARIADDTGPAE
                     LHRMILMFNRMADEIERVVARQQEFALNASHELRNPLNALLLRVEVLAADLGTAGAAD
                     VEKIREEGQRMTRILDTLLGFARGAVRASDFEDVDLTALVARRGEAWRE"
BASE COUNT          821 a         1806 c         1882 g          810 t
ORIGIN      
        1 ccgcgctgct ggacgcgctg ctgcgcttcc gcgccggcac tgcctcccag gcagaggtcg
       61 tcgacgcgtt ccggcctgcg cgcgtgctgg tgccgctggt cgcagagaag ggcgacgagg
      121 gaatcggccc gacgggcctg aaggtcgaca agacgcagga gctgtcgatc gtcaccgtcg
      181 ccgcgcccga cgggcgccgg gttcagcccg cgttctcctc ggtcgacacc atgcgccggt
      241 gggatgccca ggcccgtccc atcccggtgg aggcggcgcg cgtggcgctg tcggccgccg
      301 gcgaggagac cgacctgatc gtgctggatc cggcatccga caccgagttc gtgatccgcc
      361 gccccgcggt gtgggcgatc gcgcagggtc agccctggga gccgagcttc ctctcgcccg
      421 aggtcttctc ggcgctccgc gagagcatcg cgcacgagct ggccgtgatc gacgtgtcgg
      481 tcgcgtcagg ggatccggat gcccggatgc gcggccccga gctgatcgtg acgctcgagc
      541 tcatcgacgg cctggaccgc gagaccctgg acgccgtgct cgcgcggctc gcgcagcgct
      601 gggccgccga cgaccgcatg gcggtgctgg tcgactccct gaccgtgaag ctgacgcgct
      661 ccgccggctg acccttctcg cctcgcctca tccaaggtcg gatgcccttg gagcgcgggg
      721 ccgcaggaag gtccccggag cgagcatcgg gaggaggccc gcgcagcggg aaggaacccg
      781 ctctgcgagc gaagggaacc ttccggaggt cgtcagagca gagggctcag gtgaccgggc
      841 cggtccactt ctctcccgga cccttgccga tcgggtcctg gatgggggag gcctcgcgga
      901 aggcgagctg cagggagcgc aggccgtcgc gcagcgggct tgcgtgcgcg ctgctgatct
      961 cgggggctcc ggcggtgatc aggccggcca gtgcgttgat gagcttgcgg gcctcgtcga
     1021 ggtcgagctg cgcgtccggg tcgtccgcga gccccagctt cacggcggcc gcgctcatca
     1081 gatgcacggc ggtcgtggtg atcacttcga cggccggaac atcggcgatg tcgcgcgtcg
     1141 cggcggctgc ggcttgctcc tgctccgccc agcgctcgtg gcggtcatcg gcgtgctcag
     1201 caggaatcgt cacttcggga tgctttctgg tagacttctg cgggcttcag agtgtcatgc
     1261 tctgatacga aagaggattc attcccaccc gcgcttgccg ttccaggcta ccgggttcca
     1321 caccccgccg gactccggtg gaaagggtgt cacaagccgg tgcttctgaa tgcgtcggcg
     1381 ggtggggaga ctccgatttc gctccggaat gcagacagca tccggatggc cgaaaccacg
     1441 tctaaggagc tccgcatcag cgatccccgt accaatgagc gcatccgcgt ccccgaggtc
     1501 cgcctcgtcg gccccgcggg tgagcagatc ggtgttgtcc gcatcgaggc agcgctgcgt
     1561 ctggcccagg aggcagatct cgacctggtc gaggtcgccc cgaattccaa gccgcccgtg
     1621 gtcaagatca tggactacgg caagttcaag tacgaggccg cgcagaaggc gaaggaagca
     1681 cgccgcaacc aggcgaacac catcctcaag gaggtccgct tccgcctgaa gatcgaggct
     1741 cacgactaca cgaccaagct caagcgcgcc gagggcttcc tgaaggccgg cgacaaggtc
     1801 aaggccatga tcctgttccg tggtcgcgag cagtcgcgtc ccgagcaggg tgtgcgtctg
     1861 ctgcgcaagt tcgccgagga tgtcgccgag ttcggcaccg tggagtcgaa ccccacaatc
     1921 gacggccgca acatggtcat ggtcgtcgct ccgctgaaga acaagtccga ggccaaggcc
     1981 gagcagaacg ccgtgcgcac cgccaacaag caggcggccc gcgaggcgaa gagcagcacg
     2041 ggcgcggcat ccgccgagtc cacggacgag accgcggcgt aagccgcccc acagctcccg
     2101 ctcctggcgg gtaacccacc gtcgcccgcc agggcgccac acgaaggaag agaagatgcc
     2161 gaagcagaag acccactcgg gtgcgaagaa gcgcttcaag atcaccggca gcggaaagct
     2221 gaagaagcag caggccggca tgcgccacaa cctcgagcac aagtcgagcc gccagacccg
     2281 tcgcctgaac caggaccagg tcctctccaa ggccgacacc aaggtcgcca acaagcttct
     2341 cggccgcggc tgagcgcccg aacgcaggaa taggaacaca ggaaaatggc tagagtcaag
     2401 cgcgcggtca acgcgcacaa gaagcgtcgc gtcatcctcg agcgcgcctc gggctaccgc
     2461 ggtcagcgtt cgcgtctgta ccgcaaggcg aaggagcagg tcacccactc cctcgtctac
     2521 gcgtaccgcg accgtcgcaa gcgcaagggc gacttccgtc gtctgtggat ccagcgcatc
     2581 aacgccgctg cccgccagaa cggcatcacc tacaaccgct tcatgcaggg cctcggcctc
     2641 gcgggcgtgc aggtcgaccg tcgcatgctg gccgagctgg ccgtcaccga cgccgccgcg
     2701 ttcgccacgc tggtggacgt cgccaagaag gcgcttccct cggacgtcaa cgctccgaag
     2761 gccgctgcct gatctgaacg atcctgagaa cgggcgtctc cctgcgggga ggcgcccgtt
     2821 tccgcatccc ggcccccgat agaatgggcc cgtgctcgag aacccccgct ctccccgagt
     2881 ccgtgccgtc gccaagctga ccaagcgcag cgccaggacc gagacgggac tcttcctgct
     2941 ggagggaccg cagtcggtcc gcgaagcgct gaactacctg cccggcgcga tcgtcgagct
     3001 gttctccacg ccgtcggcgt gggagaagca ctccgatgtc cgggcgctgg cgtcggaaca
     3061 cggcatcgat gtcgagtacg tcaccgagca ggtgctcgcg gcgatggcgg acacggtcac
     3121 gccgcagggc atcgtcgcgg tcgcacggca gacgcccacc tctgtgcgcg agatcttcgc
     3181 ggcggggccg cggttgatcg cgatctgcga ggaggtgcgc gatcccggca acctgggcac
     3241 gatcatccgt gccgcggatg ccgcgggagc ggatgccgtg gtgctgaccg gtcgcaccgt
     3301 cgacccgtac aacccgaagg tggtccgctc gaccaccggt tcgctgttcc acctgccgtt
     3361 ctcggtgggc ggtgacctga acgacgtcgt ctcgcgcgcc cacgacgcgg gccttcaggt
     3421 gctcgccgct gacgtgaagg gtgacgacct cctggccgcg cgtgccgagg gcgtgctgga
     3481 gcgtcccacc gcatggctgt tcgggaacga ggcgcgcggg ctcgaggacg acatgctcga
     3541 cctcgccgac cgggcgctga agctgccgat ctacggccgg gccgagtcgt tgaacctcgc
     3601 caccgcggcc agcgtgtgcc tgtacgagag cgccttcgcg cagcgcgtgg ccggatgatc
     3661 cgatgcgcat cctgatcgtc gaggacgacg atcgtgtcgc cgaggcgctg caggccttcc
     3721 tgaaccgctc gggctatgcg accgtccgcg ccgtcgacgg cgctcaggcg ctcgggctgc
     3781 tcggctccga cacggaggtc gtgctgctgg atctggggct gcccgacatg gacggcatcg
     3841 acgtctgccg gcgcatccgt gcgcagagcg gcgtgcccgt gatcatcgcg accgcgcgca
     3901 gcgacgtgca ggagcgcatc cgcggtctgc gcgccggtgc agacgacttc gtcgtgaagc
     3961 cctacgacgt gcgggagctc gtcgcccgca tcgaggcggt cacgcgccgg ctgcatccgc
     4021 tggactcgca ccatccggcc tcgggcgacc ggtcgctgat cgaagtgggg gacgtgagca
     4081 tcgacctggt cgcgcggcgg gtgctcgtcg acggcgcccc ggtggagctg acgcgcaagg
     4141 agttcgacat catcgccgtg ctcgcccgct acccgggcgt cgccgtgccc cgcgaccggc
     4201 tcatccgtga ggtgtggaac accgactggc agagcttcgc tcgctcgctg gaggtgcacg
     4261 tcgcctcgat ccgccggaag atcgggcggg cgtcgctgat cgagaccgtc cgcggcgtcg
     4321 ggtaccgcct ggagggctga cccgtgcgcc gcaggctgat cgtcgtcttc ctcgtaccgc
     4381 tggtgctcgt gctcatggtg ctcgggggag cgtacgcgtg gagcgcggcg cgcagcgtgc
     4441 agcaggagtt ctatgccgag cagctgggtg atctcagcta cttcacgacc accgcccgtc
     4501 aggcactgct ctccggcagc gccgaggtgt tcgacggcga ggcgcagcgc tatcgggagc
     4561 tgtacggcac ccgcgtggtg atcgtggacc gcgccggccg gccctgggct ccgtccaccg
     4621 cgcaaccggt gttcgacagc cccgagatcg aggcgcagat caagctcgcg ctttcgggcc
     4681 gtcggggcga gttgccgcag aacgcgctgc cgtggcagtt ctccgacctg tcgctggtgg
     4741 agccggtgtt cgacggcggc gacgtcatcg gggcggtcgt catgtcggcg agcgtggacg
     4801 tgccgcgtgc cgagatcgga cggcagtggc tgctgatcac ggcggtcgcc acccttgtga
     4861 tcgcggtggg tctgctcgtg gtgttccggc tggcgggctg ggtgctgcgc ccggtgcggc
     4921 gtctggaccg ggcgatggag gcgatggagc gcggtgatat ggaggcccgg atcgcggacg
     4981 atacgggccc cgccgagctg catcgcatga tcctgatgtt caaccgtatg gccgacgaga
     5041 tcgagcgggt cgtcgcccgc cagcaggagt tcgccctgaa cgcctcgcac gagctgcgca
     5101 atccgctgaa cgcactgctg ctgcgggtgg aggtgctcgc cgccgacctc ggcacggcgg
     5161 gtgctgccga cgtggagaag atccgcgagg aggggcagcg gatgacgcgg atcctcgaca
     5221 ccctgctcgg cttcgcccgg ggtgcggtgc gcgcatccga cttcgaggac gtcgatctca
     5281 cggccctggt cgcccggcgg ggcgaggcgt ggcgcgagg
//
LOCUS       DATMSX010000455         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101782,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000455 DATMSX010000000
VERSION     DATMSX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101782"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2267)
                     /locus_tag="VN041_14610"
     CDS             complement(<1..>2267)
                     /locus_tag="VN041_14610"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30312.1"
                     /translation="TAQATGEDSLALGSGAGASEVGASAVGARAQASGAYSTATGTEA
                     NASGGQALASGFRSAASADGTTAVGGYAEASGRLGTALGYGSAASAANTTAVGFGAAA
                     AGTGAVAVGQSSEASGEESVAIGGSTFFGLIPSRASGTGAAAFGAGAWATEDYATAIG
                     WNSWAAGSSATALGANATANGSNSVALGAGSKADRDNTVAVGKAGGERQVTHVAAGTE
                     ATDAVNKGQLDAVASVADKTSRQFQASGNADGEAGALVDGDNALAAGNAANAIGNGAT
                     ALGSGANAMAANATAVGFDALATGNNAAALGNAAQAGGVDSVAVGSGASASELGASAT
                     GAGAQAKGAYSTASGAQATASGTQSQASGFRANASADGASALGSYAEASGRLGTAAGY
                     GSKATGANATAVGVSATASAAGSVALGAGSVADRANTVSVGSASSDRQITRVAAGTER
                     TDAVNRGQLDAVAATADGTARYVKATGAGTASANGSDAAAIGSAATASGARSNALGAN
                     AMAIGNGALALGTSAGASGANSVALGAGSRALDANVVSVGGGNGTDGPATRRIVNVAD
                     GRIAAGSSDAITGSQLNVTNQRVGAVETRVGDFDSRIATVETTAASAVSYDDAGRDRL
                     TLSGFQGTTIDNVGAGSIAAGSRQAINGGQLFQSLSDAASFLGGGASVGMQGVFVAPN
                     YVIQGATYSNVGDALGALDRKVSEIDRRTAGGTRAGTASLRAMSTPLDDSPLASAADA
                     PATADAAPASTARVQ"
BASE COUNT          294 a          822 c          789 g          362 t
ORIGIN      
        1 cctgcacgcg cgcggtgctg gccggtgccg cgtcggccgt tgcaggtgca tcagcggcgc
       61 tggccagcgg gctgtcatcc agcggggtac tcattgcgcg caggctggca gttccggcac
      121 gcgtgccacc ggcggtgcgg cggtcgatct cgctgacctt gcggtccagt gcgccgagcg
      181 catcgccgac gttgctgtag gtggcgccct ggatcacata gttcggcgcc acgaacacac
      241 cctgcatgcc aacgctggca ccaccgccga ggaagctggc ggcatcactg agcgactgga
      301 acagctggcc gccgttgatc gcctggcgac tgcccgcagc gatgctgccg gcaccgacgt
      361 tgtcgatggt ggtgccctgg aaaccggaca gggtcaggcg gtcacggccg gcgtcgtcat
      421 agctgaccgc gctggccgcc gtggtttcca cggtggcgat gcgcgaatcg aaatcaccga
      481 cgcgggtttc caccgcgccc acccgctggt tggtgacgtt gagctgcgaa ccggtgatgg
      541 catcgctgct gccggcggcg atgcggccat cggccacatt gacgatgcgg cgcgtggccg
      601 gaccatcggt gccattgcca ccgccgaccg acaccacgtt ggcatcgagt gcacgcgaac
      661 cggcacccag cgccaccgag ttcgcgcccg atgcgccggc gctggtgccc agtgccaggg
      721 caccgttgcc gatggccatc gcattggcac ccagggcatt gctgcgtgcg ccgctggcag
      781 tggctgccga cccgatcgcg gcggcatcgc tgccgttggc actggcggta cctgcgccgg
      841 tggccttgac gtagcgggcc gtgccatcgg cggtcgccgc gaccgcatcg agctggccgc
      901 gattgaccgc atcggtgcgc tcggtaccgg cagcgacacg ggtgatctga cgatcactgc
      961 tcgcgctgcc gaccgacaca gtattggcac ggtcggcgac cgagcccgcg cccaacgcca
     1021 cgctgcctgc ggcactggcg gtggcactca cacctaccgc cgtggcattg gcgccggttg
     1081 ccttgctgcc gtagccggcc gcggtaccca ggcgaccgct ggcctcggca tagctgccca
     1141 gcgcagacgc accatcggcc gaggcattgg cgcggaaacc actggcctgt gactgggtgc
     1201 cgctggctgt ggcctgcgca ccgctggcgg tactgtaggc acccttggcc tgtgcaccgg
     1261 cgccggtagc actggcgccg agttcgctgg cgctggcgcc actgcccacg gctacgctgt
     1321 cgacacctcc ggcctgcgcc gcgttgccga gcgccgccgc attgttgccg gtggccagcg
     1381 catcgaagcc cacggcagtg gcattggcgg ccatggcatt ggcgccactg cccagcgcgg
     1441 tggcgccgtt gccgatggcg ttggccgcat tgccggcagc cagtgcgttg tcgccgtcca
     1501 ccagtgcacc ggcttcgcca tcggcattgc cgctggcctg gaactgccgg ctggtcttgt
     1561 cggcgacgct ggcaaccgca tccagctggc ccttgttcac cgcatcggtg gcttcggtgc
     1621 cggccgcgac gtgggtcacc tggcgctcac cgccggcctt gccgacggct acggtgttgt
     1681 cacggtcagc cttggagccg gcgccgagcg cgacgctgtt gctgccattg gcagtggcgt
     1741 tggcacccag cgcggtggcg ctgctgccgg ccgcccacga gttccagcca atcgcggtcg
     1801 cgtagtcctc ggtggcccat gcaccggcgc cgaacgccgc cgcaccggtc ccactggcac
     1861 gcgacgggat caggccgaag aaagtgctgc cgccgatggc cacgctctcc tcgccactgg
     1921 cttcgctgga ctgacccacg gcaacggcac cggtaccggc cgccgcggca ccgaagccga
     1981 ctgcagtggt gttggcagcg ctggccgccg agccatagcc cagagcggtg ccgaggcgac
     2041 cgctggcttc ggcgtagcca cccaccgcgg tggtgccatc agccgaagcc gcgctgcgga
     2101 aaccgctggc gagcgcctgc ccgccgctgg cattggcttc tgtacctgtg gctgtgctgt
     2161 aggcgccgct ggcctgtgca cgcgcaccca ccgcgcttgc accaacctcg ctggcgccgg
     2221 caccgctgcc cagggccagg ctgtcctcgc cggtggcctg tgccgtg
//
LOCUS       DATMSX010000456         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_167814,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000456 DATMSX010000000
VERSION     DATMSX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167814"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VN041_14615"
     CDS             complement(<1..1051)
                     /locus_tag="VN041_14615"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWU30313.1"
                     /translation="MSTDRYSPAPARGAYRAPESGRPVHASVTIPGSKSLTNRELMIA
                     AIADGPGHLLRPLHSDDSRRMIDALRLLGVGVEEGASDGEFGPDLLVTPAPLKGGVTI
                     DCGQAGTVMRFIAPLAGLAAQDVHLTAHETALHRPMGALITGLRDLGVDIDDEGTWSL
                     PFTVRGHGRIRGGRVEIDASGSSQFVSGLLLAAPRFDVGLHLVHTGEHLPSLPHIDMT
                     IESLSRRGIRIERPAVGEWLVEAGVPRAKDVAIEPDLSNAAPFLAAAMVTGGEVTVAG
                     WPLHSTQPGALLPEILHAMGAHTSRHAGALTVRAGDGIRGLDIDLSAASELTPTIAGL
                     AAFADGPTTIRGVAHI"
     gene            1102..1770
                     /locus_tag="VN041_14620"
     CDS             1102..1770
                     /locus_tag="VN041_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWU30314.1"
                     /translation="MSATLTTDAVDSSAQELLARLDWPVMDETTTADPRRDFEEQAIP
                     FMDQLYAAAMRMTRNPADAADLVQETFVKAYGAWSSFTQGTNLKAWLYRILTNTYINI
                     YRKRQREPFQGTIDDLEDWQLGGAESTTASHSRSAEAEAIDRMPASAVKDALQQVPED
                     FRLAVYLADVEGFSYQEIADIMKTPIGTVMSRLHRGRRMLRELLADYAAERGIAAAQP
                     RSTK"
     gene            1767..2012
                     /locus_tag="VN041_14625"
     CDS             1767..2012
                     /locus_tag="VN041_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWU30315.1"
                     /translation="MTDCGCEKARADLEEYLRNEICTTEHSEIREHLENCEFCRSEAL
                     VATTLTEVVARACKESAPEELREQVFARLRAAQAAAH"
BASE COUNT          327 a          684 c          739 g          272 t
ORIGIN      
        1 ggatgtgcgc caccccgcgg atcgtcgtgg ggccgtcggc gaacgccgcg agtccggcga
       61 tcgtcggtgt cagctcgctg gccgcggaca ggtcgatgtc cagaccgcgg atcccgtcgc
      121 ccgccctgac cgtgagcgca cccgcatgcc tgctggtgtg cgcgcccatg gcgtgcagga
      181 tctccggcag cagcgcgccc ggctgcgtgg agtgcagcgg ccatcccgcc accgtgacct
      241 cgccaccggt gaccatcgcc gcggccagga acggggccgc gttggacaga tccggctcga
      301 tcgcgacatc cttcgcgcgg gggaccccgg cctcgacgag ccactcgccg acggcggggc
      361 gctcgatgcg gatgccgcga cggctgagcg actcgatggt catgtcgatg tgcgggaggc
      421 tggggaggtg ctcgccggtg tggacgaggt gcagcccgac atcgaaccgc ggcgccgcga
      481 ggagcaggcc agagacgaac tggctcgagc cggaggcgtc gatctccacc cggcctccgc
      541 ggatgcggcc gtgcccgcgc accgtgaagg gcagcgacca ggtgccctcg tcatcgatgt
      601 cgacgcccag atcgcgcagg cccgtgatga gtgcgcccat ggggcggtgc agcgcggtct
      661 cgtgcgcggt gaggtgcacg tcctgcgcgg cgaggccggc cagcggcgcg atgaagcgca
      721 tcacggtgcc ggcctggccg cagtcgatgg tcactccgcc cttcaggggg gcgggggtga
      781 cgagcaggtc ggggccgaac tcgccgtcgg aggcgccctc ctcgacgccg acgccgagca
      841 gccggagcgc gtcgatcatg cgacgcgagt cgtccgagtg cagcggccgc agcaggtggc
      901 ccggtccgtc ggcgatggcg gcgatcatca gctcgcggtt cgtcagcgac ttcgaaccgg
      961 ggatcgtcac ggaggcgtgc acggggcgtc cgctctccgg cgcgcggtac gcaccgcggg
     1021 cgggggcggg ggaataccgg tcagtgctca tcggttctaa gcctagtgaa gcctgcgaca
     1081 cacggagaga agaggtgacg gatgtccgcg acgctgacga cggatgccgt cgactcctcc
     1141 gctcaggagc tcctcgcccg cctagactgg ccggtgatgg acgagacgac aaccgcggac
     1201 cctcgacggg atttcgagga gcaggcgatc cccttcatgg atcagctcta cgcggcggcg
     1261 atgcgcatga cgcgcaatcc cgccgacgcg gctgacctcg tgcaggagac cttcgtgaag
     1321 gcgtacgggg cgtggtcctc gttcacgcag ggcacgaacc tgaaggcgtg gctgtaccgc
     1381 atcctgacga acacctacat caacatctac cgcaagcgcc agcgggagcc gttccagggc
     1441 acgatcgacg acctcgagga ctggcagctg ggcggggcgg agtcgaccac agcatcccac
     1501 agccgctcgg ccgaggccga ggcgatcgac cggatgccgg cgtcggccgt caaggacgcg
     1561 ctgcagcagg tgcccgagga cttccgcctc gcggtgtacc tcgcagacgt cgaggggttc
     1621 tcctaccagg agatcgccga catcatgaag acccccatcg gcaccgtgat gagccgtctg
     1681 caccgtggca ggcgcatgct ccgcgagctg ctggcagact atgccgccga gcgaggaatt
     1741 gcggcggctc aaccgaggag cacgaaatga ccgactgcgg ctgcgagaag gcgcgagctg
     1801 acctggagga gtacctccgc aacgagatct gcacgacgga gcattccgag atccgcgagc
     1861 acctggagaa ctgcgagttc tgccggagcg aggcgctggt cgcgacgacg ctcaccgagg
     1921 tcgtggcgcg cgcctgcaag gagagcgctc ccgaggagct gcgcgagcag gtcttcgcgc
     1981 gtctgcgcgc cgcccaggcc gccgcgcact gacccgctcc gc
//
LOCUS       DATMSX010000457         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_121163,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000457 DATMSX010000000
VERSION     DATMSX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121163"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VN041_14630"
     CDS             <1..615
                     /locus_tag="VN041_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWU30316.1"
                     /translation="RALAREPQLMLLDEPFSALDTGLRSATRQAVSAILAAAGTTAIL
                     VTHDQSEALSFADQVAVMRDGDLVQTGHPFVVYTRPVDRGVAEFLGEAIVLDAWLDGS
                     LASCALGYIPVRNPPAQGHVKLMLRPEQVQIVADAPVRGTVEDGVYFGAESSVRIRLE
                     SRADADPRLSAEPPTITIRHWNAALAKPGRRIALRVVGEGVVLQ"
     gene            complement(671..1699)
                     /gene="ilvC"
                     /locus_tag="VN041_14635"
     CDS             complement(671..1699)
                     /gene="ilvC"
                     /locus_tag="VN041_14635"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWU30317.1"
                     /translation="MSTEIFYDDDADLSIIQGKKVAIVGYGSQGHAHAMNLRDSGVEV
                     AIALKEGSKSIAKAEEAGFPVKTVADATAWADVIMILAPDQHQRGIYTESIRDNLTAG
                     KALAFAHGFNIRFGYIDAPEGVDVILIAPKAPGHTVRREFVAGRGIPDIIGVERDATG
                     GAWALALSYAKAIGGTRAGVIKTTFTEETETDLFGEQAVLCGGVSHLVQAGFETLTEA
                     GYQPQIAYFEVLHELKLIVDLMWEGGIAKQRWSISDTAEFGDYVSGPRIIDAGVKERM
                     KEVLGDIQSGAFAKRFIDDQDNGAAEFLALREKEQGHPIETTGKELRALFAWKQSDED
                     YVEGSAAR"
     gene            complement(1741..2250)
                     /gene="ilvN"
                     /locus_tag="VN041_14640"
     CDS             complement(1741..2250)
                     /gene="ilvN"
                     /locus_tag="VN041_14640"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWU30318.1"
                     /translation="MASHVLSLLVEDTPGILTRVAGLFARRGFNIESLAVGVTEVSGI
                     SRITVVVDVEDLPLEQVTKQLNKLINVIKIVELEQSSSVQREHVLIKVRTDNNTRSNV
                     LEVVNLFRASVVDYAPDALVVEVTGDQGKVQALLRALEPFGIKELAQSGLLAIGRGGK
                     SITERVLRG"
     gene            complement(2253..4058)
                     /locus_tag="VN041_14645"
     CDS             complement(2253..4058)
                     /locus_tag="VN041_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWU30319.1"
                     /translation="MTPPDSAPAVPRPPARTASAPEISGAEAVVRSLEKLGVTDVFGI
                     PGGAILPVYDPLMDASELRHILVRHEQGAGHAAEGYASASGRVGVCIATSGPGATNLV
                     TAIADAYMDSVPLVAITGQVFSTLMGTDAFQEADIVGITMPVTKHSFLVKDAADIPGA
                     IAAAFEIASTGRPGPVLVDITKDAQQDIVPFIWPPKVELPGYRPVTKAHGKQIQAAAT
                     LLAEAKKPVLYVGGGVIRGRASDELRILAESTGAPVVTTLMARGAFPDSHPQHLGMPG
                     MHGTVPAVLALQEADLIVSLGARFDDRVTGKVALFAPNAKVVHVDIDPAEISKIRTAD
                     VPIVGDVRDVLVDLDTAFRSTVSSDGPDTDEWWSYLDGLKSEFPLGFAPTSDGLLSPQ
                     HVIQRIGELTGPEAVYAAGVGQHQMWAAQFIKYERPNSWLNSGGAGTMGYSVPAAMGA
                     KVAEPDRVVWSIDGDGCFQMTNQELATCVINNIPIKVAIINNSSLGMVRQWQTLFYDG
                     RHSNTDLNTGAGTVRIPDFVKLGEAYGCLAIRVEKEDEVDAAIQLALETNDRPVLIDF
                     VVSADAMVWPMVPQGVSNSYVQYARDHAPAFEQED"
     gene            complement(4126..5859)
                     /gene="ilvD"
                     /locus_tag="VN041_14650"
     CDS             complement(4126..5859)
                     /gene="ilvD"
                     /locus_tag="VN041_14650"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWU30320.1"
                     /translation="MSSQEAPSGAPGAPAEIDIKPRSRVVTDGIEATTSRGMLRAVGM
                     GDADWDKPQIGIASSWNEITPCNLSLDRLAQGAKEGVHSGGGYPLQFGTISVSDGISM
                     GHEGMHFSLVSREVIADSVETVIMAERLDGTVLLAGCDKSIPGMLMASARLGLSSVFL
                     YAGSIAPGWVKLSDGTEKDITIIDSFEGVGACRAGRMTEEDLKRIECAFAPGEGACGG
                     MYTANTMASVAEALGLSLPGSAAPPSADRRRDYFAHRSGEAVVNLLRQGITTKDILTK
                     ESFENAVALAMALGGSTNVVLHLLAIANEAGVELSLHDFNRIGDRTPHVGDLKPFGKY
                     VMNDVDRHGGIPVIMKAMLDEGLLHGDALTVTGKTVAENLRELNPDPIDGEVIRTFDN
                     PIHTSGGITILHGSMAPEGAVVKSAGFDADVFEGPARVFERERAAMDALAAGEINAGD
                     VVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLCIGHIAPEA
                     VDAGPIAFVRDGDLIRVDIAARSLELLVEEAELSSRREGWEPLPPRYTRGVLAKYSRL
                     VRSAAQGAVTG"
     gene            complement(5869..>6141)
                     /locus_tag="VN041_14655"
     CDS             complement(5869..>6141)
                     /locus_tag="VN041_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWU30321.1"
                     /translation="RTGDRILEFSSRHEGKDAAITALRARTGATGILFAGDDVTDEDA
                     LRVLTEHDLGVRVGPGETAATLRVGSPQQIGELLGVIATERAAGRE"
BASE COUNT          890 a         2036 c         2200 g         1015 t
ORIGIN      
        1 cgcgccctgg cgcgcgagcc gcagctgatg ctcctggacg agccgttctc cgcgctcgac
       61 acgggcctgc gctccgccac caggcaggcg gtctccgcca tccttgccgc ggcggggacg
      121 accgcgatcc tggtgacgca cgaccagtcc gaggccctga gcttcgcgga ccaggtcgcg
      181 gtcatgcgcg acggcgatct ggtgcagacc gggcatccgt tcgtggtcta cacccgcccg
      241 gtcgaccgcg gcgtcgccga gttcctcggc gaggcgatcg tgctggacgc gtggctggac
      301 ggctctctgg cgtcctgcgc actcgggtac atcccggtgc ggaacccgcc ggcgcagggg
      361 catgtgaagc tcatgctgcg ccccgagcag gtgcagatcg tcgccgacgc gcccgtgcgg
      421 ggcaccgtcg aggacggcgt gtacttcggc gcggagagct ctgtgcgcat ccggctggag
      481 tcgcgggcgg acgccgaccc gcgcctgtcg gcggagccgc cgaccatcac catccggcac
      541 tggaacgccg cgctcgcgaa gccagggcgg cgcatcgcgc tgcgcgtggt cggcgagggc
      601 gtcgtcctgc agtgatctga acaagaaagg gcatccccga agggatgccc tttctcactc
      661 ggacggtggg tcagcgcgca gcgctgccct cgacgtagtc ctcgtcggac tgcttccagg
      721 cgaacagggc gcgcagctcc ttgcccgtgg tctcgatcgg gtggccctgc tccttctcgc
      781 gcagcgcgag gaactcggca gcgccgttgt cctggtcgtc gatgaagcgc ttggcgaagg
      841 cgccggactg gatgtcgccc agcacctcct tcatgcgctc cttgactccg gcgtcgatga
      901 tgcgcgggcc ggagacgtag tcgccgaact cggcggtgtc ggagatcgac cagcgctgct
      961 tggcgatgcc gccctcccac atcaggtcga cgatcagctt gagctcgtgg agcacctcga
     1021 agtaggcgat ctgcggctgg tagcccgcct cggtgagggt ctcgaagccc gcctgcacga
     1081 ggtggctgac gccaccgcag agcacggcct gctcgccgaa caggtcggtc tcggtctcct
     1141 cggtgaaggt cgtcttgatg acgccggcac gggtgccgcc gatggccttc gcgtaggaca
     1201 gggcgagcgc ccaggcaccg ccggtcgcgt cgcgctcgac tccgatgatg tccgggatgc
     1261 cgcgacccgc gacgaactcg cgacgcacgg tgtggcccgg tgccttcgga gcgatgagga
     1321 tgacgtcgac gccctcgggc gcgtcgatgt agccgaagcg gatgttgaag ccgtgcgcga
     1381 aggcgagggc cttgcccgcg gtcaggttgt ccctgatcga ctcggtgtag atgccgcgct
     1441 ggtgctggtc cggcgcgagg atcatgatga cgtcggccca ggcggtggcg tcggcgaccg
     1501 tcttgacggg gaagcccgcc tcctcggcct tggcgatcga cttggagccc tccttgagag
     1561 cgatggcgac ctcgacaccc gagtcgcgca ggttcatcgc gtgggcgtgg ccctgcgagc
     1621 cgtagccgac gatggcgacc ttcttgccct ggatgatcga gaggtcagcg tcgtcgtcgt
     1681 agaagatctc ggtgctcacg gtgttgtttc tccttggttg tggttcgtga agttgggggg
     1741 tcagccgcgc aggacgcgct cggtgatgct cttgccgccg cggccgatgg cgagcaggcc
     1801 cgactgggcg agctccttga tgccgaaggg ctcgagagcc ctcagcagcg cctgcacctt
     1861 gccctgatcg ccggtgacct cgaccaccag cgcgtcgggc gcgtagtcca cgacggaggc
     1921 gcggaacagg ttcacgacct cgagcacgtt cgagcgggtg ttgttgtcgg tgcgcacctt
     1981 gatgagcacg tgctcgcgct gcacggacga cgactgctcg agctcgacga tcttgatcac
     2041 gttgatcagc ttgttcagct gctttgtgac ctgttcgagc ggcaggtcct cgacgtcgac
     2101 gacgaccgtg atccgggaga tcccggacac ctccgtcacg cccacggcga gggattcgat
     2161 gttgaagccg cggcgggcga agagcccggc gacacgggtc aggatgccgg gggtgtcctc
     2221 caccagcagg ctcagcacgt gcgatgccat ggtcagtcct cctgctcgaa ggccggcgcg
     2281 tggtcgcgcg cgtactggac gtagctgttg ctgacgccct gggggaccat cggccacacc
     2341 atggcatccg cgctcacgac gaagtcgatc agcaccgggc ggtcgttggt ctccagcgcc
     2401 agctggatcg cggcgtccac ctcgtcctcc ttctcgaccc ggatcgcgag gcagccgtac
     2461 gcctcaccga gcttgacgaa gtcggggatg cggacggtgc cggcccccgt gttgaggtcg
     2521 gtgttcgagt ggcggccgtc gtagaacagc gtctgccact gccgcaccat cccgagcgac
     2581 gagttgttga tgatggcgac cttgatcggg atgttgttga tgacgcaggt cgcgagctcc
     2641 tggttggtca tctggaagca tccgtcaccg tcgatcgacc acacgacgcg gtcgggctcg
     2701 gcgaccttcg cgcccatcgc ggccgggacc gagtagccca tcgtgccggc gccgccggag
     2761 ttcagccagg agttggggcg ctcgtacttg atgaactgcg ccgcccacat ctggtgctgc
     2821 cccacgcccg ccgcgtacac ggcctccggc ccggtcagct cgccgatgcg ctggatcaca
     2881 tgctgcggcg agagcagtcc gtcactggtg ggcgcgaagc cgagcgggaa ctccgacttc
     2941 aggccgtcga ggtacgacca ccattcgtcg gtgtccggcc cgtcgctgct caccgtgctg
     3001 cggaaggcgg tgtcgaggtc gacgagcacg tcgcgcacgt cgcccacgat cggcacgtcc
     3061 gccgtgcgga tcttggagat ctcggccgga tcgatgtcga cgtgcacgac cttcgcgttc
     3121 ggggcgaaca gcgccacctt gccggtgacg cggtcgtcga agcgcgctcc gagcgagacg
     3181 atcagatccg cctcctgcag cgcgagcacg gccgggaccg tgccgtgcat gccgggcatg
     3241 ccgaggtgct gcgggtgcga gtcagggaag gcgccgcggg ccatcagcgt cgtcacgacc
     3301 ggcgcgccgg tcgattcggc gaggatgcgc agctcgtcgg acgcgcggcc gcggatcacg
     3361 ccgccgccga cgtacagcac gggcttcttg gcctcggcga gcagggtcgc cgcggcctgg
     3421 atctgcttgc cgtgcgcctt ggtcaccgga cggtagccgg gaagctccac cttgggcggc
     3481 cagatgaacg gcacgatgtc ctgctgcgcg tccttggtga tgtccacgag cacgggaccg
     3541 ggacgacccg tcgaggcgat ctcgaacgcc gccgcgatcg cgcccgggat gtcggccgcg
     3601 tccttcacga ggaaggagtg cttggtcacc ggcatcgtga tgcccacgat gtcggcctcc
     3661 tggaacgcgt ccgtgcccat cagcgtggag aacacctggc cggtgatcgc gaccagcggc
     3721 acggagtcca tgtacgcgtc ggcgatggcg gtgaccaggt tggtcgcgcc gggaccggag
     3781 gtggcgatgc agacaccgac gcggcccgag gccgaggcgt agccctccgc cgcgtgcccg
     3841 gcgccctgct cgtggcgaac caggatgtgc cgaagctcgg atgcgtccat cagcgggtcg
     3901 tacaccggga ggatcgcacc gcccgggatg ccgaagacgt cggtgacgcc cagcttctcg
     3961 agtgagcgga cgaccgcctc ggcacccgag atctcgggag cggatgcggt gcgcgccggg
     4021 ggccgcggca cggccggtgc ggaatcggga ggtgtcattt cattcctctg atgatcgtga
     4081 tgagcaggta cccgaacggg agcggagggt gtgcctgtct caggctcagc cggtcaccgc
     4141 tccctgtgcg gcagagcgca cgagacggga gtacttggcg aggacgccac gggtatagcg
     4201 cgggggtagg ggctcccagc cctcacggcg ggagctcagc tcagcctcct cgacgagtag
     4261 ctcgagggag cgagccgcga tatcgacccg tatcagatca ccatcgcgca cgaaggcgat
     4321 aggacctgcg tccaccgctt cgggtgctat gtggccgatg cacaggccgg ttgtgccgcc
     4381 tgagaatcgt ccgtccgtca agagtagtac atcttttccg agccccgcgc ccttgatggc
     4441 tccggtgatc gcgagcatct cgcgcatgcc cgggccgccc ttcgggccct cgtagcggat
     4501 gacgaccacg tcgccggcgt tgatctcgcc cgcggcgagg gcgtccatcg cggcgcgctc
     4561 gcgctcgaac acgcgggccg ggccctcgaa gacgtcggca tcgaaaccgg ccgacttcac
     4621 gacggcaccc tcgggagcca tcgatccgtg caggatcgtg atgccgccgg aggtgtggat
     4681 cgggttgtcg aaggtgcgga tgacctcgcc gtcgatcggg tcggggttca gctcgcgcag
     4741 gttctccgcg acggtcttgc cggtgacggt gagcgcgtcg ccgtgcagca ggccctcgtc
     4801 gagcatggcc ttcatgatca cggggatgcc gccgtgacga tcgacgtcgt tcatgacgta
     4861 cttcccgaac ggcttgaggt cgccgacgtg cggggtccgg tcgccgatgc ggttgaagtc
     4921 gtgcaggctc agctcgacgc cggcctcgtt ggcgatggcc agcaggtgca gcacaacgtt
     4981 cgtcgagccg ccgagggcca tggcgagggc gacggcgttc tcgaaggact ccttcgtcag
     5041 gatgtccttg gtggtgatgc cctggcgcag caggttgacg acggcctcgc cggagcggtg
     5101 cgcgaagtag tcgcggcggc ggtcggcgga gggcggcgcg gcggagccgg gaaggctcag
     5161 gccgagggcc tcggcgacgg acgccatggt gttggcggtg tacatgccgc cgcacgcgcc
     5221 ctcacccgga gcgaacgcgc actcgatgcg cttgaggtcc tcctcggtca tccggccggc
     5281 gcgacacgcg cccacgccct cgaacgagtc gatgatcgtg atgtccttct cggtgccgtc
     5341 cgagagcttc acccagcccg gtgcgatgga accggcgtac aggaacacgc tcgacaggcc
     5401 caggcgggcg cttgccatca gcatgccggg gatggacttg tcgcagccgg ccagcagcac
     5461 ggtgccgtcc aggcgctcgg ccatgatgac cgtctcgacg gagtcggcga tgacctcgcg
     5521 gctcaccagc gagaagtgca tgccctcgtg gcccatcgag atgccgtcgg agacggagat
     5581 ggtgccgaac tgcagcgggt atcccccacc ggagtgcacg ccctccttgg cgccctgtgc
     5641 gagccggtcg aggctcaggt tgcacggggt gatctcgttc cagctggacg cgatgccgat
     5701 ctggggcttg tcccagtccg cgtcgcccat gcccacggca cggagcatgc ctcgggacgt
     5761 ggtcgcctcg atgccgtcgg tgacgacccg gctgcgcggc ttgatgtcga tttcagcggg
     5821 cgcgccgggc gcgccggagg gggcttcttg ggaggacatg acggaagtct attcccgacc
     5881 ggcggcgcgc tctgtcgcga tgacgccgag gagttcgccg atctgctgtg gggatcccac
     5941 acgcagggtc gccgcggtct cccccgggcc gacccgcacg ccgagatcgt gttcggtgag
     6001 cacccgcagc gcgtcctcgt cggtgacgtc atcgccggcg aacaggatgc cggttgcccc
     6061 ggtgcgggcg cgcagcgccg tgatcgccgc gtccttgccc tcgtgccggg aggagaactc
     6121 caggatgcga tcgcccgtgc g
//
LOCUS       DATMSX010000458         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_111206,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000458 DATMSX010000000
VERSION     DATMSX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111206"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VN041_14660"
     CDS             complement(<1..179)
                     /locus_tag="VN041_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30322.1"
                     /translation="MSSTARRATIADVAREAGVSTSTASVVFSGKVNVADATREKVLA
                     AAAALGYTGPDPRAAS"
     gene            238..1461
                     /locus_tag="VN041_14665"
     CDS             238..1461
                     /locus_tag="VN041_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30323.1"
                     /translation="MDTALTRSQFVRWRAAIFAIFLASGLSISTWASRVPDIADSLGI
                     DRAQIGLLLLGMGISSIVGISTGPLVMARTGARLGMLITMLMFGSGVCLIGLGADQFG
                     SIPVVVLGMVLFGFGNGSVDVMMNVEATAIEQQMGRTILPVFHAFFSFGTVIGAGAGV
                     LATGLGLTVAAHAGIMSAIIITAAIVCFFQVPVREAALDPTPDVKPSFGQRVHTALEA
                     WREPRTYALGIVMLGMSFAEGGANDWIALGVVQGHHQESGMGALALAVFSVGMTIVRL
                     FGGPLVDRFGRVAVLRVLACAAASGILLFILGPNLPLVLIGAALWGIGASLGFPLGMS
                     AAADDPAKAAARVSAAATIGYVAFLGGPPVLGVISEHIGLLNTLFILVALVVMSGLFS
                     GAARPLRTGEPVATK"
     gene            1471..2166
                     /gene="nucS"
                     /locus_tag="VN041_14670"
     CDS             1471..2166
                     /gene="nucS"
                     /locus_tag="VN041_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HWU30324.1"
                     /translation="MRLVIARCSVDYTGRLNAHLPLATRLLVHKGDGSLLVHSDGGSY
                     KPLNWMSPPCTLAPEVPGEEEASAGVVEVWRVTHKKTGDALRVQIYEILHDSNHDLGI
                     DPGLQKDGVEADLQRLLAEQVDLISEGGTLVRREYPTAIGPVDLMIRDAGGAAIAVEV
                     KRRGDIDGVEQLTRYLELLGRDPHLAPITGVFAAQEIKPQARVLAEDRGIRCVVLDYE
                     DMKGTASGAPRLF"
     gene            2257..2706
                     /locus_tag="VN041_14675"
     CDS             2257..2706
                     /locus_tag="VN041_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30325.1"
                     /translation="MNTRPVIRALALGAILLPAALLATGCSSVIDVVVHHQQDLTFED
                     RAAMKEGWNRFEEWVPADATHIIGTAGTDGPHAAIMFRSEEDLDPAVCAEVPRRSAPV
                     YALKDAPAVYEMDKVFACGDWSVVRTDDGWLGWTPNAPDEAAESPAS"
     gene            2827..3495
                     /locus_tag="VN041_14680"
     CDS             2827..3495
                     /locus_tag="VN041_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWU30326.1"
                     /translation="MPSSPYTCILWDVDGTIADASAGILPRLRQVFASYGLPEPDAGE
                     LALWIGPPMYVSFQERAGMDEEQAHEAVSRYRELAAADGYAASVDIYPGVPEVLRAVQ
                     DAGIPQATASTKPENQVRAILEHYELIDAFTALSGARPTPEGRADGKSFVIGQALERL
                     RAAGADTSRPVLVGDRHHDVEGGAEHDIPVIFVGWGFGGEGEADAAAFRASDPASLRT
                     LLLH"
     gene            3613..>3948
                     /locus_tag="VN041_14685"
     CDS             3613..>3948
                     /locus_tag="VN041_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30327.1"
                     /translation="MAEAPTTREVQLSVPTADTTVAEWLDLQYSASESIRRLIRESVA
                     REGFTDVANRPVRPPANPVYVAESFPADVEEPADEPAAPKATAPKTAKSPASEASAPE
                     GAAPKPAARK"
BASE COUNT          556 a         1436 c         1357 g          599 t
ORIGIN      
        1 gacgcggccc gcggatcggg cccggtgtag cccagcgcgg cagcggcggc gagcaccttc
       61 tcgcgggtgg catcggcgac gttcaccttg ccgctgaaca cgaccgaggc cgtcgacgtc
      121 gagacgccgg cctcgcgggc cacgtcggcg atggtcgcac ggcgtgcggt gctgctcatc
      181 ctccgaggat aacccgccgc gtctcgcatc atcgaatcga ttcgatacac tggcccgatg
      241 gacaccgcgc tcacccgttc gcagttcgtg cgctggcgcg cggcgatctt cgcgatcttc
      301 ctcgccagcg gcctctcgat ctcgacctgg gcctcgcgcg tgcccgacat cgcggactcc
      361 ctcggcatcg accgcgccca gatcggcctg ctgctgctcg gcatgggcat ctcgtcgatc
      421 gtgggcatct ccacgggccc actggtcatg gcgcgcaccg gagcgcgtct cgggatgctg
      481 atcacgatgc tcatgttcgg ctcgggtgtc tgcctgatcg gcctgggagc cgaccagttc
      541 ggctccatcc cggtcgtcgt gctcggcatg gtgctgttcg gattcggcaa cggctcggtc
      601 gacgtgatga tgaacgtcga ggccacggcc atcgagcagc agatgggccg cacgatcctg
      661 ccggtgttcc acgccttctt cagcttcggg acggtgatcg gtgcgggcgc cggcgttctc
      721 gccaccggcc tgggcctcac cgtggcggcg cacgccggca tcatgagcgc gatcatcatc
      781 accgccgcga tcgtctgctt cttccaggtt ccggtgcgcg aggccgccct cgacccgacg
      841 cccgacgtga agccgagctt cggtcagcgc gtgcacaccg cgctcgaggc ctggcgggag
      901 ccgcgcacct acgccctggg catcgtgatg ctgggcatgt ccttcgccga gggcggagcg
      961 aacgactgga tcgcgctggg ggtcgtgcag ggacaccacc aggagtcggg catgggcgcg
     1021 ctcgcgctgg cggtcttctc ggtgggcatg accatcgtgc ggctattcgg cggaccgctc
     1081 gtggaccgct tcggccgcgt ggcggtgctg cgcgtgcttg cctgcgccgc ggcatccggc
     1141 atcctgctct tcatcctcgg cccgaacctg cccctcgtgc tgatcggtgc cgcgctgtgg
     1201 ggcatcggcg cctccctcgg cttcccgctg ggcatgtccg cggctgccga cgatcccgcc
     1261 aaggccgctg cacgagtgag cgcggcggcc acgatcggct acgtcgcctt cctcggcggc
     1321 ccgccggtgc tcggcgtgat cagcgagcac atcgggctgc tcaacacgct gttcatcctc
     1381 gtggcgctgg tcgtcatgtc ggggcttttc tcgggcgcgg cgcgcccgct gcgcaccgga
     1441 gagcccgtcg cgacgaagta ggctcttcgg gtgcgtctcg tcatcgcccg ctgctccgtc
     1501 gattacaccg ggcggctcaa cgcccatctg cccctcgcca cgcgcctgct ggtgcacaag
     1561 ggcgacggca gcctgctcgt gcactccgac ggcggcagct acaagccgct gaactggatg
     1621 agcccgccgt gcaccctggc tcccgaggtg ccgggcgagg aggaggccag cgccggcgtc
     1681 gtcgaggtgt ggcgcgtcac ccacaagaag accggcgacg ccctgcgcgt gcagatctac
     1741 gagatcctgc acgactccaa ccacgacctg ggcatcgacc ccggcctgca gaaggacggc
     1801 gtcgaggccg acctgcagcg cctgctggcc gagcaggtgg atctgatcag cgagggcggc
     1861 acgctcgtgc gccgcgagta cccgacggcc atcggccctg tcgacctgat gatccgggat
     1921 gccggcggtg cggcgatcgc cgtggaggtc aagcgccgcg gcgacatcga cggcgtggag
     1981 cagctcaccc gatacctcga actgctgggc cgcgacccgc acctggcgcc catcaccggc
     2041 gtgttcgccg cgcaggagat caagccgcag gcgcgcgtgc tcgccgagga ccgcggcatc
     2101 cgctgcgtcg tgctcgacta cgaggacatg aagggcaccg cctccggcgc cccgcgcctg
     2161 ttctgagcga cgacgcgcag ggatcatccc ggcggcgtac gcgggatcac tccgcaggct
     2221 gacgatcggc gcgcgccgct ccggaaggct ggaagcatga acacgcgtcc cgtcatccgc
     2281 gccctcgcgc tcggtgccat cctgctcccc gccgccctcc tcgccaccgg ctgctcatcc
     2341 gtgatcgacg tggtggtgca ccaccagcag gatctgacct tcgaggaccg cgccgcgatg
     2401 aaggaggggt ggaaccgctt cgaggagtgg gtgccggccg atgccacgca catcatcggc
     2461 acggcgggca ccgacggccc gcacgcggcg atcatgttcc gcagcgagga ggacctcgac
     2521 ccggcggtgt gcgccgaggt tccgcgacgg tccgcccccg tgtacgcgct gaaggacgcc
     2581 cccgccgtct acgagatgga caaggtcttc gcctgcggtg actggtccgt cgtccgcact
     2641 gacgacggct ggctcggctg gacgccgaac gccccggacg aggccgccga gtcccccgca
     2701 tcctgatccg gcatcacggc ggaaggccat gactccggag aatctcgccc ccgaagcccg
     2761 aatcggctcc gacgaggcgg gaccacgccg ggttctccgg agttgtgtcc gccgtaggct
     2821 ggacacatgc cctcctcgcc ctacacctgc atcctctggg acgtggacgg caccatcgcc
     2881 gacgcgtcgg ccggcatcct gccgcggctg cgccaggtgt tcgcctcgta cggactgccg
     2941 gaaccggatg ccggtgagct ggcgctctgg atcggaccgc ccatgtacgt gtccttccag
     3001 gagcgggctg gcatggacga ggagcaggcg cacgaggcgg tgtcccggta ccgggagctc
     3061 gccgcggcgg acggctacgc ggcatccgtc gacatctacc cgggcgtccc cgaagtcctg
     3121 cgagcggtgc aggatgccgg catcccgcag gccaccgcca gcaccaagcc cgagaaccag
     3181 gtgcgcgcga tcctcgagca ctacgagctg atcgacgcct tcaccgcgct gtccggcgcg
     3241 cgcccgacgc ccgagggccg cgccgacggc aagtcattcg tgatcgggca ggcgctggag
     3301 cggctgcgcg cggccggtgc cgacacctca cggcccgtgc tggtgggcga ccgccaccac
     3361 gacgtcgagg gcggcgccga gcacgacatc ccggtgatct tcgtcggctg gggcttcggc
     3421 ggggagggtg aagcggatgc cgcggcattc cgagcatccg atcccgcatc gctgcgcacc
     3481 cttctgctgc actgatccgc cccgcacgac cggtcggttc tgcgacggta tcggctcggc
     3541 ggacagcctc gtgctgtacc gtgattatgc gcgtgccctg gtaatcgcgc gacgaggaga
     3601 gagggaaccg cagtggctga agcaccgacc acccgcgagg tgcagctcag cgtccccacc
     3661 gccgacacca ccgtggccga atggctcgat ctgcagtact ccgcttcgga gtcgatccgc
     3721 cgcctgatcc gcgagtccgt ggcccgcgag gggttcaccg acgtggcgaa ccggccggtg
     3781 cgtccgccgg cgaatccggt gtacgtggcc gagagcttcc ccgctgacgt cgaggagccc
     3841 gccgacgaac ccgcggcgcc gaaggcgacc gccccgaaga cggcgaagag ccctgcatcc
     3901 gaggcatccg ctccggaggg cgcagcgccg aagcccgccg cccgcaag
//
LOCUS       DATMSX010000459         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_177753,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000459 DATMSX010000000
VERSION     DATMSX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177753"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VN041_14690"
     CDS             <1..705
                     /locus_tag="VN041_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWU30328.1"
                     /translation="LQLPALGGGVLAGVASALGGRFAFRTGIRLSTRDVASLLFAFLV
                     IGTLFAVLTATDTTWWDRSFSQLGIGAGGWAFNGTVIIAGLLIATTGSYVGRDLHRML
                     GDTALRGIAVVVVAWAGAGLALAAVGLLPLDQVPVPHAIAAFATLGLIVAAAIATTVL
                     MGELRMLRVLTVVLVVLIAVAVVLAFVLRMLSVAALEAVAVGLVLLWLSTLVQLLGIL
                     APDESRPSSRRSPLRA"
     gene            complement(723..1385)
                     /locus_tag="VN041_14695"
     CDS             complement(723..1385)
                     /locus_tag="VN041_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWU30329.1"
                     /translation="MSMTAPEALAELAALEDPKARAVNERHGDAHGVNLSKMRGLAKR
                     IGLSTDLARSLWDSDDVAGQLLALLIAKPRELSADEIDRMLRSTRSAKAHDWLVNYIA
                     KKSPLAEELRLRWSDDADADARAAHWSLTSTRVQKTPDGLDLDALLDRIERELKDAPE
                     REQWAMNETLAYIGIHHPALRDRAVTIGERLQVLADYPTPPNCTSPFAPIWIAEMVRR
                     QG"
     gene            1533..>2814
                     /locus_tag="VN041_14700"
     CDS             1533..>2814
                     /locus_tag="VN041_14700"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30330.1"
                     /translation="MSTSTATPRIRSALFILMLAMVAEGLDLVVASFVFPRVMEEWGT
                     SIEDITTLVTLSVVAGAVGGYLAGPLADRFGCRPVIVVCALEFSALTIATAAVPGMAG
                     LMIVRLAACLGLGALVPVVIAQVSELVPGRTRSHMVSLVWSGGAFGFILGSVLAAVVI
                     PAAGWRVLVIVSGVLSIVLVPFLLRVLPRRAARLEEPIPATSHAEAGTAVGTLFTRRY
                     LASTLLTWICFAVGLGVAYLIATYLPLMVEQYGMTAGVAALVTGLFGLCGLLGQLGLG
                     FALRRWDPRSVLAVVWGVGTLGIVLLAVVDLGIAGFLIAISAIAFTIAGSNAVLPTLA
                     TALYPARAKATGVSWANGAGQIGRLGGGWAGGAMLAAGFSTTQLFLAIAVPTVAGIVG
                     AVLLRRARRTAEGPGDDTGLAPAESPIVAGTTTTE"
BASE COUNT          319 a          975 c         1011 g          509 t
ORIGIN      
        1 ctgcaattgc cggcgctcgg cggaggggtg ctggcgggcg tggcgtcggc gctcggcggg
       61 cggttcgcgt tccgcaccgg catccggctg agcacccgtg acgtcgcctc gctgctgttc
      121 gcgttcctcg tgatcggcac cctcttcgcc gtgctcaccg ccaccgacac gacctggtgg
      181 gaccgcagtt tctcgcagct cgggatcggc gcgggaggct gggcgttcaa cggcacggtg
      241 atcatcgcgg gtctgctgat cgcgacgacc ggttcgtatg tgggccggga tctgcatcgg
      301 atgctgggcg acaccgcgct gcgcggcatc gccgtcgtcg tggtcgcgtg ggcgggagcc
      361 ggactcgcgc tcgccgccgt cgggttgctg ccgctggatc aggtgccggt gccgcatgcg
      421 atcgccgcgt tcgcgacgct gggcctgatc gtcgcggcgg cgatcgcgac caccgtgctg
      481 atgggggagc tgcggatgct gcgcgtgctc acggtggtgc tggtggtgct catcgccgtc
      541 gcggtggtgc tcgcgttcgt cctgcggatg ctgtcggtgg ccgcgctcga ggccgtagcc
      601 gtcggactgg tgctgctgtg gctgtcgacg ctggtccagc tgctcggcat cctcgctccc
      661 gacgagtcgc gcccctcgtc ccgccgctct ccgctgcggg cgtagtggcg gcgcctgccg
      721 ggtcagccct ggcggcgcac catctccgcg atccagatcg gcgcgaacgg gctcgtgcag
      781 ttcggcgggg tggggtagtc ggcgagcacc tgcagccgct cgccgatcgt gacggcgcgg
      841 tcgcggagcg cggggtgatg gatgccgatg taggcgagcg tctcgttcat cgcccactgc
      901 tcgcgctcgg gagcatcctt cagttcgcgc tcgatgcggt cgagcagagc gtcgaggtcc
      961 aggccgtccg gggtcttctg cacccgcgtc gaggtcagcg accagtgcgc ggcccgggca
     1021 tccgcatccg catcgtccga ccagcgcagc cgcagctcct cggcgagagg cgacttcttg
     1081 gcgatgtagt tgaccagcca gtcgtgcgcc ttcgcgctgc gtgtgctgcg cagcatccgg
     1141 tcgatctcgt cggccgagag ctccctcggc ttcgcgatca gcagagcgag cagctgcccg
     1201 gccacgtcgt cgctgtccca gagggagcga gccaggtcgg tcgacagccc gatccgcttg
     1261 gcgaggccgc gcatcttcga caggttcacg ccgtgcgcat cgccgtgccg ttcgttcacg
     1321 gcccgtgcct tcgggtcctc cagcgctgcg agctcggcga gggcttcagg ggcggtcatc
     1381 gacatgagaa cagggtaggc gagaggggcc ggaacctcct gtgggatcga cgggtccggg
     1441 ttggggaatc gccgatgcga ggcgcagatc cggctcgtta gcgtgcgtgc acggcactgt
     1501 tcgtgccgac cgacccgagc acggagcttc acatgtcaac gtcgactgca acaccccgga
     1561 tccgttccgc tctcttcatc ctgatgctgg ccatggtggc cgaaggcctc gacctcgtgg
     1621 tcgcgagctt cgtcttcccc cgcgtcatgg aggagtgggg cacatccatc gaggacatca
     1681 cgaccctcgt caccctcagc gtcgtggcgg gcgcggtggg cggctatctc gccgggccgc
     1741 tcgccgacag attcggctgc cgcccggtca tcgtcgtctg cgcgctcgag ttctctgcgc
     1801 tcacgatcgc gaccgcggcc gtacccggga tggccgggct gatgatcgtc cggctcgccg
     1861 catgcctggg gctcggcgcc ctggtgcccg tcgtgatcgc gcaggtgtcc gagctcgtcc
     1921 cgggacgcac ccggtcgcac atggtgagcc tggtgtggtc gggcggcgca ttcgggttca
     1981 tcctcggaag cgtgctggcg gccgtcgtga tccccgccgc cggctggcgc gtcctcgtca
     2041 tcgtgagcgg ggtgctctcg atcgtgctgg tgccgttcct tctccgcgtg cttccgcgcc
     2101 gcgccgcgcg tctcgaggag ccgatccccg ccacctccca tgccgaagcc ggcaccgcag
     2161 tgggcaccct gttcacacgt cgttacctcg cctccacgct gctgacatgg atctgcttcg
     2221 ccgtgggact cggcgtcgcc tacctcatcg cgacgtatct gccgctcatg gtcgagcaat
     2281 acggcatgac cgcaggcgtg gccgcactgg tcaccgggct gttcggcctg tgcggactgc
     2341 tcggccagct cggtctcgga ttcgcgctga ggcgctggga cccgcgctcc gtactcgccg
     2401 tggtgtgggg cgtcggcacc ctcgggatcg tgctgctcgc cgtggtcgac ctcggcatcg
     2461 cgggtttcct catcgcgatc tcggcgatcg cgttcaccat cgctggatcc aatgccgtgc
     2521 ttccgactct cgccaccgcc ctgtaccccg cacgcgcgaa ggcgacgggc gtctcgtggg
     2581 ccaacggagc ggggcagatc ggccggctcg gcggcggctg ggccggggga gccatgctcg
     2641 ctgccggctt cagcaccacg cagctgttcc tggccatcgc ggtgccgacc gtggcgggga
     2701 tcgtcggcgc ggtgctgctg cgacgtgccc gccggacggc agaaggaccg ggggatgaca
     2761 cgggcctcgc gcctgcggag agcccgatcg tcgccgggac caccacgacc gagg
//
LOCUS       DATMSX010000460         6457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_92707,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000460 DATMSX010000000
VERSION     DATMSX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92707"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VN041_14705"
     CDS             complement(<1..805)
                     /locus_tag="VN041_14705"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWU30331.1"
                     /translation="MMSTAPQARSAGLRYVFPVLAGKLARFATRLRGGGSAFPGYLTN
                     RLAPSLLPTLADQFRYGVVFVLGSNGKTTTTHMVSEVLRAHGLTVFTNPTGANLPQGV
                     TSALLADATLTGRIKADVAVLEIDEGYAADLADRLSPSVILSLNVQVDQLYRFYETER
                     VADMMLDASTRASAHVVVNRDDPYLSKIDVDAMRAPVSFFGVAPEIIAASAHGLVNAA
                     DTRSGNAGTAERDAYSEVRSVNGREVSIRIGDADAAVRLPARGLHYAADE"
     gene            complement(802..2049)
                     /locus_tag="VN041_14710"
     CDS             complement(802..2049)
                     /locus_tag="VN041_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWU30332.1"
                     /translation="MTPATTKKTRTTTKKTAAAEVETPEVEEAAEAAAAKAPAKKAPA
                     KKAPVKRASKKKDETPEVDDEAVDAADADDEENDKKPAFTEPLPTGAIVISSADEDDV
                     PVYSTQITGATADPVKDYLKQIGKVALLNAAEEVELAMRIEAGLFAEEKLSTMSAAEK
                     SSQLGLDLQWVARDGQRAKSHLLGANLRLVVSLAKRYTGRGMQFLDLIQEGNLGLIRA
                     VEKFDYTKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQD
                     LGREPTPEELSRELDMTPEKVVEVQKYGREPISLHTPLGEDGDSEFGDLIEDTEAVVP
                     ADAVGFTMLQRQLEQLLDSLSEREAGVIRMRFGLGDGQPKTLDQIGDTFGVTRERIRQ
                     IESKTMAKLRHPSRSQSLRDYLE"
     gene            complement(2216..3148)
                     /locus_tag="VN041_14715"
     CDS             complement(2216..3148)
                     /locus_tag="VN041_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWU30333.1"
                     /translation="MPFSDELYESIAGAPDVPTGLPLVILLTGFTDAGNAVSGLIEHL
                     HDTTDPQPLIVFDNDVLLDYRARRPVIVFDQDHLTEFRPARLELALAHDALGQPFLLL
                     SGYEPDFAWNAFTAAVVSFAEGFQVSAVTWVHSIAMPVPHTRPLGTTVSGNRRDLIAA
                     HSVWRPRTQVPATAGHLLEYRLIQQDVRVAGFVLLVPHYLADTEYPDTVITAADKLMI
                     ATGLVLRMDEVQERREEYLARVEEQVHGSEELMQMVHTLERRYDAYMAGRDDEGDEAD
                     ESFDERDLPSADELAAELERYLANRRPGDDEKRG"
     gene            3261..4736
                     /locus_tag="VN041_14720"
     CDS             3261..4736
                     /locus_tag="VN041_14720"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWU30334.1"
                     /translation="MTLPVISHTTDPFPGSAADAAVLVIPDPAGAHDALDGYEGLTGT
                     LSAIGYKGTASAYTRVHLPAVTDLPLAVVSAGATPSAENVRDAVATAVRSLTGFATVS
                     IGLAPGLEQFADAAAEGALLGGYRFDDYRTEKKPARADALVLHAPSIGDGALENARIV
                     AEAVALVKDLVSVPAEWQSPAQLAQSAADAVASLPVQVEILDEKQLEEQGFGGIIGVG
                     RGSDRPPRLVKLSYSPEGAGRHIALVGKGITFDTGGLSLKPAASMLGMKFDMAGSATV
                     LAAIRAIAALALPVRVTAWMCIADNMPSGRATRPGDVLRMLDGQTVEVLNTDAEGRLV
                     MADGLVAASRENPDVVFDVATLTGAIITALGKRHTGVMGEDAAVAEYLAASEQTGEPA
                     WQLPLPEYMEELLESPIADMINSNMSDRSGGSMFAGLFLQRFVGRTSDAEDAPRIPWV
                     HLDIAGSGEHTGSPYGFTEKGPTGATVRSLIAFARASVKEA"
     gene            4736..6109
                     /gene="lpdA"
                     /locus_tag="VN041_14725"
     CDS             4736..6109
                     /gene="lpdA"
                     /locus_tag="VN041_14725"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWU30335.1"
                     /translation="MAVHEFDLVILGGGSGGYAAALRAAELGKKVALIEKDKVGGTCL
                     HRGCIPTKALLHAAEVADHVRTAASVGVTATLGGIDAAGVTAYREGIVAKKFKGLEGL
                     IKARGITVIAGEGRLEKDRSVSVGDDRYVGADVVLATGSYSRSLPGLEIGGRILTSEQ
                     ALALEEIPAKVIVLGGGVIGVEFASVWHSFGAEVTIVEALPHLVPNEDIVLSKGLERA
                     FRRKGISYNLGVRFQKAEQDENQVTVTLEDGKTFTADYLLVAVGRGPATAGLGFEEAG
                     ATVDRGFVIVDEQLRTGVPGLWAVGDIVPGLQLAHRGFLQGIAVAERIAGMEVPALPE
                     SQIPRVTYCSPEVASVGITEEAAIAAHGADAVVSYEYNLAGNGKSEIIGTGGTVKVVR
                     QKDGPVLGVHLLGDRVGELITEGQLAVAWEAHPEDIAPLIHAHPTQSEALGEAFLALA
                     GKPLHAL"
     gene            6177..>6457
                     /locus_tag="VN041_14730"
     CDS             6177..>6457
                     /locus_tag="VN041_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWU30336.1"
                     /translation="MSTPVVLPALGESVTEGTVTRWLKQVGDTVQADEGLLEISTDKV
                     DTEIPSPVSGVIEEILVAEDETVEVGALLARIGDGSGAGSTGDAPAAAAP"
BASE COUNT          966 a         2214 c         2270 g         1007 t
ORIGIN      
        1 tctcgtcggc ggcgtagtgc agaccgcgcg cgggcaggcg cacagcggca tccgcgtcgc
       61 cgatcctgat cgacacctcg cgtccgttca cgctgcgcac ctcggagtac gcgtcgcgct
      121 ccgcggtgcc ggcgttgccg ctgcgggtgt ccgcggcgtt gacgagaccg tgcgcggatg
      181 ccgcgatgat ctcgggtgcg acgccgaaga acgacaccgg ggcgcgcatg gcgtcgacgt
      241 cgatcttgct gaggtacggg tcgtcgcggt tcacgacgac gtgggcggat gcccgcgtcg
      301 aggcgtcgag catcatgtcg gcgacgcgct cggtctcgta gaagcgatac agctggtcga
      361 cctgaacgtt cagcgacagg atgaccgacg gggacagccg gtcggccaga tcggccgcgt
      421 agccctcatc gatctcgagc accgctacat cggccttgat ccgcccggtg agggtggcgt
      481 cggcgagcag tgcactggtc acgccctgcg gcaggttggc tccggtcggg ttcgtgaaca
      541 cggtgagccc gtgtgcgcgc agcacctcgc tgaccatgtg cgtggtcgtg gtcttgccgt
      601 tcgagcccag cacgaacacc acgccgtagc ggaactggtc cgcgagcgtg ggcagcagcg
      661 acggggcgag ccggttggtg agatagccgg ggaaggcgga gcctcccccg cgcagacggg
      721 tcgcgaagcg cgcgagcttt cccgccagca cggggaagac gtaccgcagg ccggctgagc
      781 gcgcctgagg tgccgtcgac atcattcgag gtagtcccgc agcgactgcg accggctcgg
      841 gtggcggagc ttggccatgg tcttcgactc gatctggcgg atgcgctcgc gcgtcacgcc
      901 gaacgtgtcg ccgatctggt cgagcgtctt cggctggccg tcgcccaggc cgaagcgcat
      961 ccggatcaca ccggcctcgc gctcggacag ggagtccagg agctgctcga gctgacgctg
     1021 aagcatcgtg aagcccacgg cgtcggcggg cacgacggcc tcggtgtcct cgatcagatc
     1081 accgaactcg ctgtcaccgt cctcgccgag gggcgtgtgc agcgagatcg gctcacggcc
     1141 gtacttctgc acctcgacga ccttctcggg cgtcatgtcc agctcgcggg agagctcctc
     1201 gggcgtgggt tcgcgaccca ggtcctgcag catctggcgc tgaacgcggg cgagcttgtt
     1261 gatgacctcg accatgtgca ccgggatgcg gatggtgcgg gcctggtcgg ccatggcgcg
     1321 ggtgatcgcc tgacggatcc accaggtggc gtacgtggag aacttgaagc ccttggtgta
     1381 gtcgaacttc tcgactgcgc ggatgaggcc caggttgccc tcctggatga gatccaggaa
     1441 ctgcatgccg cggccggtgt agcgcttggc cagcgagacg accagacgca ggttggcgcc
     1501 gagcagatgg ctcttggcac gctgaccgtc gcgggcgacc cactgcaggt cgagaccgag
     1561 ctggctggac ttctcggcgg ccgacatcgt cgacagcttc tcctcggcga acaggccggc
     1621 ctcgatgcgc atggcgagct cgacctcttc ggccgcgttc agcagcgcga cctttccgat
     1681 ctgcttcagg tagtccttga ccgggtcggc cgtggcgccg gtgatctggg tggagtagac
     1741 cgggacgtcg tcctcgtcgg cggacgagat cacgatcgca ccggtcggca gcggctcggt
     1801 gaacgccggc ttcttgtcgt tctcctcgtc gtccgcatcc gcggcgtcga cggcctcgtc
     1861 gtcgacctcg ggcgtctcgt ccttcttctt ggacgcgcgc ttgacaggag ccttcttggc
     1921 gggcgccttc ttggcgggcg ccttcgcagc agccgcctct gcggcctctt cgacctcggg
     1981 ggtctcgacc tctgcggcgg ccgtcttctt ggtggtcgtc cgggtcttct tggtcgtggc
     2041 aggagtcacg tttcgccttt cacggagcgg tgcacgcgct ccgggccatt tcggacacta
     2101 gtaagaccct tgtcaagccg gatcgcgagg atcgatggtt gacaacgggt cagacgtcca
     2161 ttatcacaca catgacggca tgcggcgtcc caggtcgtga tctccgcgcg tcggctcagc
     2221 cgcgcttctc gtcgtcgccg ggtctgcggt tcgccagata ccgctccagc tccgcggcga
     2281 gctcgtcagc gctgggcagg tcgcgctcgt cgaagctctc gtcggcctca tcgccctcgt
     2341 cgtcgcgccc ggccatgtag gcgtcgtagc gccgttcgag ggtgtgcacc atctgcatca
     2401 gctcctcgct gccgtgcacc tgctcctcga cacgggccag gtactcctcg cggcgctcct
     2461 gcacctcatc catccgcagc acgaggcccg tcgcgatcat gagcttgtct gcggcggtga
     2521 tcacggtgtc cgggtactcc gtgtcggcca ggtagtgcgg cacgagaagc acgaacccgg
     2581 cgacgcgcac gtcctgctgg atgagccggt actcgagcag gtggccggcc gtcgcgggga
     2641 cctgcgtgcg cggacgccac accgagtgcg cggcgatcag gtcgcgccgg ttgccgctga
     2701 cggtcgtgcc cagcggacgg gtgtgcggca cgggcatggc gatcgagtgc acccaggtga
     2761 ccgcggaaac ctggaatccc tccgcgaagc tcaccacggc ggccgtgaag gcgttccagg
     2821 cgaagtcggg ctcatagccc gacagcagca ggaagggctg gccgagcgcg tcgtgagcca
     2881 gcgcgagctc gaggcgggcg ggacggaact cggtcaggtg gtcctggtcg aagacgatca
     2941 cgggacgccg cgcgcggtag tccagcagca cgtcgttgtc gaacacgatc agcggctgcg
     3001 ggtcggtggt gtcgtggagg tgctcgatga ggccggagac cgcgttgccc gcatccgtga
     3061 agccggtgag caggatcacc aggggaaggc cggtgggcac atcgggcgcg ccggcgatcg
     3121 actcgtagag ctcatcggag aagggcatgt gtcaatgcta cgagccgggg tgccatcggg
     3181 gcgggcgcca ctcatgctga cagcgaacac gggcgccgcg gtgatcccgg gagcaaccgg
     3241 cggatcccta ggatggagac atgacgcttc ccgtgatctc gcacacgacc gatccgttcc
     3301 ctggaagtgc tgcagacgcc gcagtgctcg tcatccccga tcccgcaggc gcacacgacg
     3361 cgctggacgg ctacgagggc ctgaccggaa ccctgtccgc catcggctac aagggcaccg
     3421 catccgctta cacgcgcgtg cacctgccgg ccgtgaccga cctcccgctg gccgtcgtga
     3481 gcgcaggtgc gactccgagc gccgagaacg tacgcgatgc cgtggccacc gcggtccgca
     3541 gcctgacggg cttcgcgacc gtctcgatcg ggctcgcccc cggtctcgag cagttcgcgg
     3601 atgccgcagc cgagggcgcc ctcctcggcg gataccgctt cgacgactac cgcaccgaga
     3661 agaagcccgc ccgcgcggac gccctcgtgc tgcatgcgcc gtcgatcggc gacggcgcgc
     3721 tggagaacgc ccgcatcgtc gcggaggccg tcgccctggt gaaggacctc gtgtccgtgc
     3781 ccgccgagtg gcagagcccc gcacagctcg cccagtccgc cgcggacgcc gtcgcctccc
     3841 tgcccgtgca ggtggagatc ctcgacgaga agcagctcga ggagcagggc ttcggcggca
     3901 tcatcggcgt cggccgcggc tcggaccgcc cgccccgcct cgtcaagctc agctactccc
     3961 ccgagggcgc gggccgccac atcgcactgg tcggcaaggg gatcacgttc gacaccggcg
     4021 gcctgtcgct caagcccgcc gcgagcatgc tcggcatgaa gttcgacatg gccggctccg
     4081 ccacggtgct cgccgccatc cgcgcgatcg ccgccctggc gctgcccgtc cgggtcacgg
     4141 cgtggatgtg catcgccgac aacatgccgt cgggtcgcgc cacgcgcccc ggcgacgtgc
     4201 tgcggatgct ggacggccag acggtcgagg tgctgaacac ggacgccgag ggccgcctgg
     4261 tgatggccga cggcctggtc gccgcgagcc gggagaaccc ggatgtcgtc ttcgatgtcg
     4321 cgaccctgac cggtgcgatc atcaccgcac tcggcaagcg ccacaccggc gtcatgggcg
     4381 aggacgccgc ggtcgccgag tacctcgccg cctccgagca gaccggagag ccggcctggc
     4441 agctgccgct gccggagtac atggaggaac tgctggagtc ccccatcgcc gacatgatca
     4501 actcgaacat gtccgaccga tcgggcggct cgatgttcgc gggtctcttc ctgcagcgct
     4561 tcgtcggccg cacctcggat gccgaggacg cgccccgcat cccctgggtc cacctcgaca
     4621 tcgccggctc cggcgagcac accggctcgc cgtacggctt cacggagaag ggccccaccg
     4681 gcgccaccgt gcgctccctc atcgcgttcg cgcgcgcatc ggtcaaggag gcctgatggc
     4741 tgtacacgag ttcgacctcg tcatcctcgg cggcggcagc ggcggctacg ccgcggccct
     4801 gcgcgcagcg gagctcggga agaaggtggc gctgatcgag aaggacaagg tcggtggcac
     4861 ctgcctgcat cgcggatgca tccccaccaa ggcgctgctg cacgcggccg aggtcgccga
     4921 tcacgtgcgc accgcggcct ccgtcggcgt gaccgccacc ctcggcggca tcgacgccgc
     4981 cggcgtgacc gcgtaccgcg aggggatcgt cgcgaagaag ttcaagggcc tggagggtct
     5041 gatcaaggcg cgcgggatca ccgtgatcgc cggtgagggc cgcctcgaga aggaccgctc
     5101 ggtgagcgtg ggcgacgacc gctacgtcgg cgccgacgtg gtgctggcca ccggctcgta
     5161 cagccgcagc ctccccggtc tggagatcgg cggacgcatc ctcaccagcg agcaggcgct
     5221 cgcgctcgag gagatccccg ccaaggtcat cgtgctcggc ggcggcgtga tcggtgtcga
     5281 gttcgccagc gtctggcact cgttcggcgc ggaggtcacg atcgtcgagg ctctgccgca
     5341 cctggtgccc aacgaggaca tcgtgctgag caagggcctg gagcgcgcgt tccgccgcaa
     5401 gggcatctcg tacaacctcg gcgtgcgctt ccagaaggcg gagcaggacg agaaccaggt
     5461 cacggtcacg ctggaggacg gcaagacgtt cacggccgac tacctgctgg tcgccgtggg
     5521 tcgtggtccc gccactgccg ggctgggctt cgaggaggcg ggcgccacgg tggaccgcgg
     5581 cttcgtgatc gtggacgagc agctccgcac cggcgttccc ggcctgtggg ccgtgggcga
     5641 catcgtcccg ggccttcagc tcgcgcaccg cggattcctt cagggcatcg cggtcgccga
     5701 gcgcatcgcg ggcatggagg tgccggcact ccccgagtcg cagatccccc gagtgaccta
     5761 ctgcagcccg gaggtggcgt cggtgggcat caccgaggag gccgcgatcg cggcccacgg
     5821 cgcggacgcc gtcgtctcct acgagtacaa cctggccggc aacggcaaga gcgagatcat
     5881 cggcaccggt ggcacggtca aggtcgtgcg ccagaaggac ggccccgtcc tcggcgtgca
     5941 cctgctcggc gaccgcgtcg gcgaactcat caccgagggc cagctggccg tcgcgtggga
     6001 ggcgcatccg gaggacatcg ctccgctgat ccacgcgcac cccactcaga gcgaggcgct
     6061 gggcgaggct ttcctcgctc tggccggaaa accgctccac gcactctgaa ccgcaccaac
     6121 agggcgcccg ggtcactaag cttggggcgt cacagatttc tgaaggagac tccgtcatga
     6181 gcacacccgt ggtccttccc gcacttgggg agagcgtcac cgagggtacg gtcactcgct
     6241 ggctgaagca ggtcggcgac acggtgcagg cggatgaggg cctgctcgag atctcgaccg
     6301 acaaggtcga caccgagatc ccctcgcccg tcagcggcgt catcgaggag atcctcgtgg
     6361 ccgaggacga gaccgtcgag gtgggcgctc tgctcgcccg catcggcgat ggcagcggag
     6421 ccggctcgac cggcgacgcg cccgcagccg cggcacc
//
LOCUS       DATMSX010000461         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_73586,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000461 DATMSX010000000
VERSION     DATMSX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73586"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..965)
                     /locus_tag="VN041_14735"
     CDS             complement(162..965)
                     /locus_tag="VN041_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU30337.1"
                     /translation="MTGILERGTAAIRYSDGGGSGRPVILTHGAGMDSASFAAQAEAV
                     QDAGFRSIRWDLRAHGASTLAPGARFTAQDALADLGALIDELRLESPVLVGHSLGGNL
                     SQAYVRTQPGRASGLIAVGTTWNTGPLGRGERFGLRMAAPLLRLIPGAKLPALMADAS
                     AVTPEAVESIRATFARMPKSVFLEVWRATASFVAPDPEYRSPVPLGLVVGAEDRTGNI
                     RTAMQAWADAEGVRLHAIPAAGHVVMADAPDETSAAIVGILREWDAAAR"
     gene            complement(992..>4136)
                     /gene="mfd"
                     /locus_tag="VN041_14740"
     CDS             complement(992..>4136)
                     /gene="mfd"
                     /locus_tag="VN041_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWU30338.1"
                     /translation="ALQPVASNLGDAAPLELRVGGRGIELEDAVEQLVERAYSRVDMV
                     SRRGEFAVRGGILDVFPPTADHPARVEFFGDEVDQIRAFSVADQRSLPGDVASVELPA
                     SRELLLTADVRERAAALVGRFPAISGMLEKMAEGIPVEGMESLLPAVAGPLMTLAEYL
                     PEGSATAIVDPERASTRAQNLGETNREFLDAAWSAATSGASAPIDLGAGDFRTIAQLR
                     DIVRGREGVWWRLSPFAIDDADAANLDSSVIPSFHGNVDGAIAFVEARLGEGWRVVVI
                     ASGHGLVERARDVLSDRGLAARIVEDLVDAPEGGIASLVVGSVESGFQVPEAKLAVLT
                     DSEFYGRTIGGDQRVVKKLASRRRNVVDPLQLKQGDYVVHTTHGIGRFVEMTKREVSS
                     GGRNAVKSTREYLVLEYAPSKRGYPGDKLFVPTDQLDLLSKYVGGEAPALSKMGGSDW
                     SAAKGKARRAVRDIAVELVKLYSARVNSKGYAFGPDTPWQRELEEAFPFAETHDQLQT
                     IDEIKADMERPIPMDRLLSGDVGFGKTEVAVRAAFKAIQEGKQVAMLVPTTLLVKQHL
                     ETFTERFAGFPVKVRPLSRFQTDKEARLTLQGLVDGSVDMVIGTHRILTDQVMFKDLG
                     LMIIDEEQRFGVEHKDALKKMKTNVDILAMSATPIPRTLEMAVTGIREMSTLQTPPED
                     RHPILSFVGPRSDKQIAAAIRREILREGQVFFVHNRVQSIQRVASELAELVPEARIAV
                     AHGRMGEHQLEQVVDDFWERKYDVLVSTTIIETGLDISNANTIIIDKADKYGLSQLHQ
                     LRGRVGRGRERAYAYFLYDDAKPLSETAADRLQTIAVNNDLGSGMQVALKDLELRGAG
                     NLLGAEQAGHIAGVGFDLYLRMIGEAVATFRGEETSSDVELRLELPVDARIPEHYIDS
                     ERLRLEAYQKLSAAAGAGAAEDAIDLVAEELIDRYGALPEEVETLVTVARLRRRAARA
                     GLTDVVAMGSNLRIAPARFEDSMKVRLARLYPKAKLVGGGEALVVPMPLDVELIEWVG
                     NLFTAMFPEPAKAAPVA"
BASE COUNT          618 a         1491 c         1405 g          622 t
ORIGIN      
        1 gacgcccagc gctccgccag caggcgggcg tagccccagg gcggacgctc ctccgtggcc
       61 cgatcgtcga gccagccgaa gccccagccc gtgacgtccg cggcctccgc atccgcgatg
      121 agttcctcga aggtgccgtc catccttcga tcctgacacg ctcagcgcgc tgccgcgtcc
      181 cactcccgca ggatgccgac gatcgccgcc gacgtctcgt ccggggcgtc cgccatgacc
      241 acgtggccgg cggcggggat cgcgtgcagt cgcacgccct cggcgtcggc ccacgcctgc
      301 atggctgtcc ggatgttgcc cgtgcggtcc tccgcaccga cgaccagccc cagcggcacc
      361 ggcgagcggt actccggatc gggggcgacg aatgaagccg tcgcgcgcca cacttccagg
      421 aacacggact tcggcatccg ggcgaatgtc gcacggatcg actcgaccgc ctcgggtgtc
      481 accgccgagg catccgccat gagcgcgggc agcttcgccc cagggatgag gcggagcagg
      541 ggcgccgcca tacgcagccc gaatcgctcg ccacgaccga gcgggcccgt gttccaggtc
      601 gtgccgacgg cgatcagccc actcgcgcgg ccgggctgcg tacgcacgta cgcctgagag
      661 aggttgcccc cgagggagtg cccgaccagg accgggctct ccagcctcag ctcgtcgatg
      721 agagcgccca gatcggccag cgcgtcctgt gctgtgaaac gcgcgccggg cgccagcgtc
      781 gaggcgccgt gcgcgcgcag atcccagcgg atgctgcgga atccggcatc ctgcacggcc
      841 tccgcctgcg cggcgaacga ggcggagtcc atgcccgcgc cgtgcgtgag gatgaccggc
      901 cgtccagagc cgcccccgtc gctgtaacgg atggccgccg tgccgcgctc gaggatgccc
      961 gtcatgcctc gagcctagga gatcccgttg ctcaggcgac cggcgcggcc ttcgccggct
     1021 cggggaacat cgcggtgaac aggttgccga cccactcgat cagctccacg tccagcggca
     1081 tcggcacgac gagcgcctct cccccgccga ccagcttggc cttggggtac aaccgtgcca
     1141 gccgcacctt catggagtcc tcgaaacgcg ccggcgctat gcgcaggttc gaacccatcg
     1201 cgacgacatc cgtgagaccc gcgcgagccg ccctgcgtcg caggcgtgcg acggtcacga
     1261 gcgtctcaac ctcctccggc agcgcgccgt agcggtcgat gagctcctcg gcgaccaggt
     1321 cgatcgcgtc ctcggccgcg ccggcaccgg ccgcggcgga gagcttctga taggcctcca
     1381 gacgcagccg ctcgctgtcg atgtagtgct cggggatccg ggcatccacc ggcagctcca
     1441 ggcgaagctc gacatccgac gaggtctcct cgccgcggaa cgtcgccacg gcctcgccga
     1501 tcatgcgcag gtacaggtcg aagccgacac cggcgatgtg accggcctgc tcggcgccca
     1561 gcagattgcc cgccccgcgc agctcgaggt ccttcagcgc gacctgcatg cccgagccga
     1621 gatcgttgtt caccgcgatc gtctgcagcc ggtcggccgc cgtctcgctg agcggcttgg
     1681 cgtcgtcgta gaggaagtac gcgtacgccc gctcacgacc gcggcccaca cgaccgcgca
     1741 gctggtgcag ctgcgacagc ccgtacttgt cggccttgtc gatgatgatc gtgttggcgt
     1801 tggagatgtc caggcccgtc tcgatgatcg tcgtcgagac gagcacgtcg tacttgcgct
     1861 cccagaagtc gtcgaccacc tgctccagct gatgctcgcc catcctgccg tgcgccacag
     1921 cgatgcgcgc ctcgggcacg agctccgcga gctcggatgc cacgcgctgg atggactgca
     1981 cgcgattgtg cacgaagaac acctggccct cgcgcaggat ctcgcggcgg atcgccgcgg
     2041 cgatctgctt gtcgctgcgc ggcccgacga aggagaggat cggatgccgg tcctcgggcg
     2101 gcgtctgcag cgtcgacatc tcacggatgc cggtgaccgc catctcgagc gtgcgcggga
     2161 tcggcgtcgc gctcatcgcg aggatgtcga cgttggtctt catcttcttc agcgcgtcct
     2221 tgtgctcgac gccgaagcgc tgctcctcgt cgatgatcat caggcccagg tccttgaaca
     2281 tcacctggtc ggtgaggatg cggtgcgtgc cgatgaccat gtcgaccgag ccgtccacga
     2341 gcccctgcag cgtgagccgg gcctctttgt cggtctggaa acgcgagagg ggtcgcacct
     2401 tcaccgggaa gccggcgaag cgctcggtga aggtctccaa gtgctgcttg accagcaggg
     2461 tggtcggcac gagcatcgcg acctgcttgc cctcctggat ggccttgaac gcggcgcgca
     2521 cggccacctc ggtcttgccg aagccgacgt cgccggagag cagtcggtcc atcgggatgg
     2581 gccgctccat gtcggccttg atctcgtcga tcgtctgcag ctggtcgtgc gtctcggcga
     2641 acgggaacgc ctcttccagc tcgcgctgcc agggggtgtc cggcccgaag gcgtagccct
     2701 tcgagttgac gcgcgcggaa tacagcttga ccagctccac ggcgatgtcg cgcacggccc
     2761 tgcgcgcctt gcccttggcg gccgaccagt cgctgccgcc catcttcgac agtgcgggcg
     2821 cctcgccgcc gacgtacttc gagagcaggt cgagctggtc ggtgggcacg aacagcttgt
     2881 cgcccgggta gccgcgcttg gagggcgcgt attccagcac caggtactcg cgggtggact
     2941 tgacggcgtt gcgtccgccc gaggacacct cgcgcttggt catctcgacg aaccggccga
     3001 tgccgtgggt ggtgtggacg acgtagtcgc cctgcttgag ctgaagcggg tcgacgacgt
     3061 tgcgccgccg ggacgccagc ttcttcacga cgcgctggtc gccaccgatc gtgcggccgt
     3121 agaactcgct gtcggtgagc acggcgagct tcgcctccgg cacctggaag ccggactcga
     3181 cggaaccgac cacgagcgag gcgatcccgc cctcgggtgc gtcgacgagg tcctccacga
     3241 tgcgcgcggc caggccgcgg tcgctcagca cgtcccgggc gcgctccacg agcccgtgcc
     3301 cggacgcgat cacgacgacg cgccagcctt cgcccagccg cgcctcgacg aaggcgatcg
     3361 caccgtcgac gttgccgtgg aaggacggga tgaccgagga atcgaggttc gcggcatccg
     3421 catcgtcgat cgcgaacgga ctcagccgcc accacacgcc ctcgcgcccg cgcacgatgt
     3481 cgcgcaactg tgcgatggtg cggaagtcac cggcgccgag atcgatcgga gcggatgccc
     3541 cggatgtggc cgcgctccac gcggcatcca ggaactcccg gttggtctcc ccgaggttct
     3601 gcgcgcgcgt gctggcgcgc tcggggtcga cgatcgccgt cgcggatccc tcgggcaggt
     3661 actcggccag tgtcatcagc ggccccgcga cggcaggaag caacgactcc atgccctcga
     3721 ccgggatgcc ctcggccatc ttctcgagca tccccgagat cgccgggaac cggccgacca
     3781 gtgcggcggc gcgctcgcgc acgtcggcgg tcagcagcag ctcgcggctg gcgggcagtt
     3841 cgacggatgc cacatcgccc gggagggagc gctggtcggc gaccgagaag gcacggatct
     3901 gatcgacctc gtcaccgaag aactccacgc gggccgggtg atcggccgtg ggcgggaaga
     3961 cgtccaggat gccgccgcgc acggcgaact caccacgacg cgagaccatg tccacgcggg
     4021 agtacgcgcg ctcgacgagc tgctccacgg cgtcctcgag ctcgatcccg cgccctccga
     4081 cgcggagctc gagcggagcc gcgtcgccga ggttcgaggc gacgggctgc agcgcg
//
LOCUS       DATMSX010000462         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_167882,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000462 DATMSX010000000
VERSION     DATMSX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167882"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN041_14745"
     CDS             <1..228
                     /locus_tag="VN041_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWU30339.1"
                     /translation="VLAVLDPELIVLGGPTSLAGGERLAAMVADELRSDERPDAEVRL
                     SVTGDSSVLAGARRLLVEQIRTRLEDRIPTW"
     gene            265..1521
                     /locus_tag="VN041_14750"
     CDS             265..1521
                     /locus_tag="VN041_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HWU30340.1"
                     /translation="MKLAIVGGGSTYTPELIDGFIRLQAELPIEELVLVDTDPSRLEL
                     VGGVAKRMLARGGHPTRFSTTDDLVTGVAGADAVLIQLRIGGQDARDQDETWPHDAGC
                     IGQETTGPGGLAKALRTVPVVLRIAETVRAHAKPDAWIIDFTNPVGIVTRALLQAGHR
                     AVGLCNVAIGFQRRFAAEAGVSPDRVALAHVGLNHLTWERGVFVDGVDTLPQALQGNL
                     QELADDVQLAPALLAQLGVIPSYYLRYYYAHDEVLHEQLHKSTRAHEVRAIEQELLAL
                     YADSSVDTKPEILEKRGGAYYSEAAIELLLAIRGGSELPRVVNLRNDGVLPFLPDDHV
                     IEVPARHVDGRFVAERVAPLPDDIRGLISAVAGYERLALDAAVNGGRDRVLRAMRSHP
                     LVVQHDRAEKLTDLLMAANSRFLEWA"
     gene            1518..>1966
                     /locus_tag="VN041_14755"
     CDS             1518..>1966
                     /locus_tag="VN041_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWU30341.1"
                     /translation="MTVAADLVIAVDGGGSKTDVVLLDLSGRMLGWQRGSGSSPQVEG
                     LRNSVHVIDDLVDRVLDGRDPRRLAQVGVYLSGLDMDEEIEAFRAAIAHLPWAAGPTV
                     AENDLHALLRAGTDSRNAVAVICGTGMNAIGVREDGTDVRFLALGPLS"
BASE COUNT          296 a          678 c          707 g          285 t
ORIGIN      
        1 gtgctcgccg tgctcgaccc cgagctcatc gtgctcgggg ggccgacctc ccttgccggc
       61 ggcgagcggc tcgcggcgat ggtcgccgac gagctgcgca gcgatgaacg gcccgacgct
      121 gaggtgcgtc tgagcgtcac cggcgacagc tcggtgctcg caggcgcgcg acggctgctc
      181 gtcgagcaga tccgcacccg tctcgaagac cgaatcccca cctggtgagc cacggcgcaa
      241 ccggtacgaa cacaaggaga gcacatgaaa ctggccatcg tcggcggcgg atcgacatac
      301 acgcccgaac tcatcgacgg cttcatccgc ctgcaggccg aactgccgat cgaggaactc
      361 gtcctggtcg acaccgaccc gtcgcggctc gagctcgtcg gcggtgtcgc gaagcggatg
      421 ctggcgcgcg gtggccaccc gacccggttc agcaccacgg acgacctcgt cacgggggtg
      481 gcgggtgcgg atgccgtgct gatccagctc cgcatcggcg ggcaggatgc ccgtgatcag
      541 gacgagacct ggccgcatga cgcgggctgc atcggccagg agaccaccgg gccgggcggt
      601 ctcgcgaagg cgctgcgcac ggtgcccgtc gtgctccgga tcgccgagac cgtgcgcgcg
      661 cacgccaagc cggatgcctg gatcatcgac ttcaccaacc cggtgggcat cgtcacccgg
      721 gctctgctgc aggcagggca tcgcgcggtg ggtctgtgca atgtggccat cggcttccag
      781 cgccgcttcg cggccgaggc cggtgtctcg cccgatcgcg tggcactcgc gcacgtcggg
      841 ctgaaccacc tcacctggga gcgcggggtc ttcgtcgacg gcgtcgacac cctgccgcag
      901 gctctgcagg gcaatctcca ggagctggcc gacgacgtgc agctggcgcc cgcgctgctc
      961 gcacagctgg gggtgatccc ctcgtactac ctgcgctact actacgcgca tgatgaggtg
     1021 ctgcatgagc agctgcacaa gtccaccagg gcgcacgagg tccgcgcgat cgagcaggaa
     1081 ctgctggcgc tgtatgcgga cagcagcgtc gacaccaagc ccgagatcct ggagaagcgc
     1141 ggcggcgcgt attactccga agccgcgatc gagctgctgc tggcgatccg cggcgggtcc
     1201 gagctgccgc gcgtggtcaa cctccgcaac gacggcgtgc tgcccttcct gcccgacgac
     1261 cacgtgatcg aggtgccggc gcggcacgtg gacggacgct tcgtcgccga gcgggtcgct
     1321 ccgctgcccg acgacatccg agggctcatc agcgcggtcg cgggctacga gcggctcgcg
     1381 ctggatgcgg ccgtgaacgg cggccgcgac cgcgttctcc gcgccatgcg ctcgcatccg
     1441 ctggtggtgc agcacgaccg cgcggagaag ctcaccgacc tgctcatggc ggcgaactcg
     1501 cgcttcctgg aatgggcatg accgtggcgg cggatctggt gatcgcggtc gacggcggag
     1561 gctcgaagac cgacgtcgtg ctgctcgacc tgagcggcag gatgctgggc tggcagcgcg
     1621 gctcgggatc gagcccacag gtggagggac tgaggaactc ggtgcacgtc atcgacgatc
     1681 tcgtcgaccg ggtgctggac gggcgggacc cgaggcggct cgcgcaggtg ggggtgtacc
     1741 tgtccgggct ggacatggac gaggagatcg aggccttccg cgcggccatc gcccacctgc
     1801 cctgggcggc gggccccacg gtcgcggaga acgacctgca cgccctgctg cgcgcgggca
     1861 ccgattcccg caacgccgtg gccgtgattt gcggcaccgg gatgaacgcc atcggcgtgc
     1921 gcgaggacgg gaccgacgtg cgcttcctcg ccctcggacc gctgtc
//
LOCUS       DATMSX010000463         7344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_82668,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000463 DATMSX010000000
VERSION     DATMSX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82668"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VN041_14760"
     CDS             complement(<1..452)
                     /locus_tag="VN041_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWU30342.1"
                     /translation="MTSTTTSLSAGEARRITLAAQGFSRRMPDAVASRHLHRAMDRLG
                     VLQIDSVNVFARSHYMPMFSRLGAYDTALLDRSFLSRTTHYVEYLAHEAAFMPVEDWA
                     LWDFRMRAWRDRASRPDSWMHANARTLEWVRAELRDRGPLRPADLRDDA"
     gene            522..1718
                     /locus_tag="VN041_14765"
     CDS             522..1718
                     /locus_tag="VN041_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWU30343.1"
                     /translation="MSDNPTPRFRNPFRHYPPVTDRTVTTEVDEAVPKPLRVTAAYAW
                     RLLVIAGAIALFIWLVMLLKALVIPLMVGILITALLWPAFQIMLRARFPRWLAIATTV
                     IGTIAIVAGLLWLVIWQVRAQWQDVQERTLVAIEQLKVLLLDSPFHLTASDIDGYIAQ
                     LQTLLTEQQNWLWSAAGAVTGTAAEVLTGAVLSLFILICLLADGGGIWKWTLRLFPRT
                     ARPAVDAAARNGWETVINYARTQMLVAGIDAVGIGVGAALLGVPMAIPVAVLVFLGSF
                     VPIVGAVVTGAVAVFLALVYNGPWIALAMLGVVLLVQQLEGHILQPILMGSAVKVHPL
                     AVVLVVAGGAMIARIPGALFAVPLAAFINVAAVTISTGAWRTGEEPAADLIWSTVPRE
                     RTRRNR"
     gene            1715..2944
                     /gene="ilvA"
                     /locus_tag="VN041_14770"
     CDS             1715..2944
                     /gene="ilvA"
                     /locus_tag="VN041_14770"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWU30344.1"
                     /translation="MTAVPSLAEFSAAAESLSGVISHTPTELSRALSDIVGAPVLLKM
                     ENLQRTGSFKIRGATYRLAQLTAEERSRGVVAASAGNHAQGVALAAQSLGIPATIFMP
                     LGVPVPKLLATRGYGAEVVLEGETVATSLRLAAEFAERTGAVLIPPFNHRDVIIGQGT
                     LGLEIFQDVPDVDTVIMGIGGGGLIAGVAAAIKQAAAEAGRTVRVIGVQAENAAAVPP
                     SLQAGEPVEITTHPTIADGILVARPGDIPFEIIQELVDEVVTVTEDDIARALLVLLEH
                     AKVVVEPAGAVGVAAILAGKVKGTGKTVPILSGGNIDPMLLQRVVSHGLAASGRYATV
                     RIALPDRPGQLAKVSEILAQVGANVMEVLHTRHGHGLQISEMILQLSIETRGPEHTQL
                     ALDTLRAAGFEPEIVAD"
     gene            complement(3017..3505)
                     /gene="greA"
                     /locus_tag="VN041_14775"
     CDS             complement(3017..3505)
                     /gene="greA"
                     /locus_tag="VN041_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWU30345.1"
                     /translation="MSTDAQVPFLTQEAYDRLAEELERLSTTGREEIAARIEAAREEG
                     DLKENGGYHAAKDEQGKQEARIVTLQQLLKTAKVGEAPTSRGIVEPGTVVTAIVAGGE
                     EVFLLGSREIAVGADMDVYSEASPLGQAILGLKIGEKTSYEAPNGRAISVEITGVETY
                     TG"
     gene            complement(3628..4014)
                     /locus_tag="VN041_14780"
     CDS             complement(3628..4014)
                     /locus_tag="VN041_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HWU30346.1"
                     /translation="MTTARELDERYGRTGRRRLPWIIGGTVAAVLVGAFGWMTVVNEM
                     STVSADDMGFTLNDDHSVDVRFQYTAPRGADVVCVVQALDEEFGIVGWKVLEIPASQK
                     HTSALDVRIPTVAAATTGLVKSCWVA"
     gene            4110..4400
                     /locus_tag="VN041_14785"
     CDS             4110..4400
                     /locus_tag="VN041_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30347.1"
                     /translation="MILFGDNTPMPTPTSTVPPEIVTPGFAGFAVVIVVVAAVILLIW
                     DMNRRIRRVRYREEVRDELDAELAAASDGPAGADAPAETVAPTEPDDDGAKR"
     gene            complement(4420..4893)
                     /locus_tag="VN041_14790"
     CDS             complement(4420..4893)
                     /locus_tag="VN041_14790"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HWU30348.1"
                     /translation="MADVESFTLDHTAVHAPYVRLIGVERGPKGDAISNFDVRFVQPN
                     EGEIPTAGLHTIEHTMASVLRDEIDGVIDFSPFGCRTGFHLIMWGEPAVVDVVAAVRA
                     GLEFIAGPAQWSDVPGVSAVECGNYRDHSLHSAKEWAKRILDQGISEDAFRRVGV"
     gene            complement(4968..5702)
                     /locus_tag="VN041_14795"
     CDS             complement(4968..5702)
                     /locus_tag="VN041_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWU30349.1"
                     /translation="MSTRAAGDPDVPQLPLLEAAAKDAAVEIKPSWRGWIHAGTFPVA
                     IAAGIVLIVLAHGAPGKWAAAVFMATSLLLFGNSALYHRFNWGPKTKMVLKRIDHANI
                     LLLIAGTYTPLAVLALEPGKGTLLLIVVWSGAILGILFRVFWINAPRWLYVALYLALG
                     WAAVMYMVDLVHANVAMMVLVCVGGVLYTIGAVIYAMKKPNPWPGHFGFHEIFHVCTV
                     LAFLCHWTACLLISLAPHAPSLGLAG"
     gene            5792..6574
                     /locus_tag="VN041_14800"
     CDS             5792..6574
                     /locus_tag="VN041_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWU30350.1"
                     /translation="MSARESSGRGPLYRLYGNRLRRRIDPATVPHHVAMMIDGNRRWA
                     RQLGYESAADGHRAGAAKMREFLGWCDELGVDVVSLYLLSADNLTKRDSKEIGDLIEI
                     IAELADTLSKHGDWRVQHVGRADILPPELARVLADAEERTRDHTGLHVNLAVGYGGRN
                     EIVDAVRSIIGKHNASGGTLEDLAAHLTPEMIGEHLYTGGQPDPDLVIRTSGEQRLSD
                     FLLWQSAHSEFYFVEALGPDLRRVDFLRAIRDFADRDRRFGR"
     gene            6621..>7344
                     /locus_tag="VN041_14805"
     CDS             6621..>7344
                     /locus_tag="VN041_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWU30351.1"
                     /translation="MSALDDYLASFTGETGYLNWAAFGPLAPAVRSAMHDAVEVMGGY
                     SPREHATVTARTGRAVAGIAEMLGAKRAEVVLRPSSTDALQHALYGLSGAVIAGSGEF
                     PSISLTLQRAADASSGALAPRWIDPADGRVTTDAVATALDDDVTAVAVSHVDYRTGYR
                     ADLTALRELIGPDRLLIVDAVQSFGVVDEDWSTADVVAGHGYKWLRSGRGTGFARFSD
                     RAVERLRPVLSGITGIDADGPFS"
BASE COUNT         1087 a         2547 c         2566 g         1144 t
ORIGIN      
        1 gcatcgtcgc gcagatccgc cggccgcagc gggccccgat cacgcagttc ggcgcgcacc
       61 cattcgagcg tgcgcgcgtt cgcatgcatc cagctgtccg gtcgggatgc ccggtcccgc
      121 cacgcccgca tccggaagtc ccagagagcc cagtcctcga cgggcatgaa ggcggcttcg
      181 tgggcgaggt actcgacgta gtgcgtcgtg cgggagagga acgaccgatc gagcagcgcc
      241 gtgtcgtaag cgcccagccg cgagaacatc ggcatgtagt gcgagcgcgc gaacacgttc
      301 acggagtcga tctgcagcac gcccaggcga tccatggccc ggtgcaggtg gcgggatgcc
      361 acggcatccg gcatccgccg ggagaagccc tgtgcggcga gcgtgatccg gcgcgcctct
      421 ccggcgctga gggaggtggt cgtcgacgtc acccgttcag gctatccggg ccctccgaca
      481 cggagcgggg cgctgccggg gagcatacgt agaattgcac aatgagcgac aaccccacac
      541 cgaggttccg caatccgttc cgccattatc cgccggtgac ggaccgcacc gtcaccaccg
      601 aggtcgacga ggcggtgccc aagccgctgc gcgtcaccgc cgcgtatgcc tggcgtctgc
      661 tcgtcatcgc cggggccatc gccctgttca tctggctggt gatgctgctc aaggcgctgg
      721 tgatcccgct tatggtcgga atcctgatca cggcactgct gtggccggca ttccagatca
      781 tgctccgtgc gcggttcccg cgctggctgg ccatcgccac cacggtcatc ggcacgatcg
      841 ccatcgtggc gggcctgctg tggctggtca tctggcaggt gcgtgcgcaa tggcaggacg
      901 tccaggagag gacgctggtg gccatcgagc agctcaaggt cctgctgctc gacagtccgt
      961 tccacctcac cgcctccgac atcgacggct acatcgcaca gctgcagacg ctgctcaccg
     1021 agcagcagaa ctggctgtgg agcgctgccg gggccgtcac cggcacggcg gcggaggtgc
     1081 tcacgggcgc cgtgctctcg ctgttcatcc tgatctgcct gctggccgac ggcggcggca
     1141 tctggaagtg gacgctgcgc ctcttccccc gcacggcgag gcccgccgtc gacgccgcgg
     1201 cgcgcaacgg ctgggagacc gtgatcaact acgcccgcac gcagatgctg gtcgcgggca
     1261 tcgacgccgt gggcatcggc gtcggagccg ctctcctcgg ggtgccgatg gccattccag
     1321 tggcggttct ggtgttcctc ggatcgttcg tcccgatcgt cggcgccgtc gtgaccggcg
     1381 ctgtggcggt gttcctggca ctcgtgtaca acggcccctg gatcgccctg gcgatgctcg
     1441 gtgtcgttct gctcgtgcag cagctggaag ggcacatcct gcagccgatc ctgatgggct
     1501 cggcggtcaa ggtgcatccg ctcgccgtcg tgctcgtcgt cgcgggaggc gcgatgatcg
     1561 cccgcatccc gggtgccctg ttcgccgtcc cgttggcggc cttcatcaac gtcgcggcgg
     1621 tgaccatcag caccggcgcc tggcgcacgg gcgaggagcc cgccgccgat ctcatctgga
     1681 gcacagtccc gcgtgagcgg acgaggagga atcgatgacc gcagtcccga gcctggcgga
     1741 gttctcagcc gcagccgaga gcctgtccgg ggtgatctcg cacacgccga ccgagctgtc
     1801 gcgcgcgctc agcgacatcg tcggggcgcc ggtgctgctg aagatggaga atctgcagcg
     1861 cacgggctcg ttcaagatcc gcggtgcgac gtaccggctc gcccagctca ccgctgagga
     1921 gcgctcgcgc ggcgtggtcg cggcgtcagc cggcaaccac gcgcagggtg tggcactcgc
     1981 cgcgcagtcc ctcgggatcc ccgcgacgat cttcatgccg ctgggcgtgc cggtgccgaa
     2041 gctgctggcg acgcgtggtt acggcgccga ggtggtgctg gagggggaga cggtggcgac
     2101 ctcgctgcgc ctcgcggccg agttcgccga gcgcactggt gcggtgctga tcccgccgtt
     2161 caaccaccgc gacgtgatca tcggccaggg gacgctcgga ctggagatct tccaggacgt
     2221 ccccgacgtc gacaccgtga tcatgggcat cggcggcggc gggctgatcg ccggcgtcgc
     2281 cgccgccatc aagcaggccg ccgcggaggc cggacgcacg gtgcgggtca taggcgtgca
     2341 ggctgagaac gccgccgccg tgccgccatc cctgcaggcc ggggagccgg tcgagatcac
     2401 cacgcacccc accatcgccg acgggatcct cgtcgcccgg cccggcgaca tccccttcga
     2461 gatcatccag gagctcgtgg acgaggtcgt cacggtcacc gaggacgaca tcgcccgggc
     2521 gctgctggtg ctgctcgagc acgccaaggt cgtcgtcgag ccggccggag cggtcggcgt
     2581 cgcagccatc ctcgcgggga aggtcaaggg cacaggtaag accgtcccga tcctctcggg
     2641 cggcaacatc gaccccatgc tgctccagcg cgtggtctcg cacgggctcg cggcctccgg
     2701 ccgctacgcg accgtgcgga tcgcgctgcc ggaccgcccc ggccagctcg cgaaggtctc
     2761 cgagatcctc gcgcaggtcg gtgcgaacgt catggaggtg ctgcacaccc ggcacggtca
     2821 cggccttcag atcagcgaga tgatcctgca gctctcgatc gagacccggg gccccgagca
     2881 cactcagctc gcgctggaca cgctgcgcgc ggcgggattc gagccggaga tcgtggcgga
     2941 ctagcattcg cccgccgaga cgcggaaggc gggatgcccc tcaggggcat cccgccttct
     3001 gtcgtgctgg tcgcggtcag ccggtgtagg tctccacgcc ggtgatctcg acggagatcg
     3061 cgcggccgtt gggagcctca tagctggtct tctcgccgat cttcaggccg aggatggcct
     3121 gtccgagcgg gctggcctcg ctgtagacgt ccatgtcggc gcccaccgcg atctcgcggc
     3181 tgccgagcag gaacacctct tcgccgccgg cgacgatcgc ggtgacgacg gtgccgggct
     3241 cgacgatgcc gcggctggtg ggggcctcgc cgaccttcgc ggtcttcagc agctgctgga
     3301 gcgtgacgat gcgcgcctcc tgcttgccct gctcgtcctt ggctgcgtgg tagccgccgt
     3361 tctccttgag gtcgccctct tcacgggcag cctcgatgcg tgcggcgatc tcctcgcgac
     3421 cggtggtcga gaggcgctcg agctcttcgg cgagccggtc gtaggcctcc tgcgtgagga
     3481 acggtacctg agcgtctgtg gacacagcgt gctccttcgg tgcgggcccc ttcaggggct
     3541 gaggattccg aaccgtctgc tgcggctcgg cggatatgcc aagacgcccc ggcgtgggcc
     3601 ggggcgtcgg tcgtcttgcg gacgagtcta agcgacccag caggacttca ccaaaccggt
     3661 cgtcgcagcc gcgacggtcg ggatgcgcac gtccagcgcc gaggtgtgct tctgcgaggc
     3721 cgggatctcg agcaccttcc agcccacgat gccgaactcc tcgtcgagcg cctgcaccac
     3781 gcacacgacg tcggcgccgc gcggcgcggt gtactggaag cggacgtcca cgctgtgatc
     3841 gtcgttgagc gtgaagccca tgtcgtcggc gctcacggtg ctcatctcgt tcaccacggt
     3901 catccagccg aaggcgccca cgagcacggc cgcgacggtg ccgccgatga tccagggcag
     3961 ccgtcggcga ccggtgcgac catagcgctc gtcgagttcg cgtgcggtgg tcacgggtct
     4021 cctgatcctt cgtggaggga cgggccgttc cgcatcggcg gataggctgg tactcccagg
     4081 ttattcgacc tgaggatgaa aggcgcacca tgatcctgtt cggcgacaac accccgatgc
     4141 ccacgccgac ctcgaccgtc ccgcccgaga tcgtgacccc gggtttcgcc ggcttcgccg
     4201 tcgtgatcgt ggtggtcgcg gccgtcatcc tgctgatctg ggacatgaac cgccgcatcc
     4261 gccgggtccg ctaccgcgaa gaggtgcggg acgaactgga tgccgagctg gccgcggcat
     4321 ccgatggtcc cgccggcgcc gacgcgcctg ccgagaccgt cgcgcccacc gagcccgacg
     4381 acgacggcgc caagcgctga cccgcgggct ctccccgcgt cagacgccga cgcgccggaa
     4441 ggcgtcctcg ctgatcccct gatccaggat gcgcttcgcc cactccttgg cggagtgcag
     4501 gctgtggtcg cggtagttgc cgcactccac cgccgagacg ccgggcacgt cactccactg
     4561 cgcggggccc gcgatgaact ccaggcccgc gcggaccgca gcgacgacgt cgacgaccgc
     4621 cggctcgccc cacatgatca ggtggaaccc ggtacggcat ccgaacggcg agaagtcgat
     4681 gacgccgtcg atctcgtcgc gcagcacgct cgccatggtg tgctcgatgg tgtgcagtcc
     4741 cgcggtgggg atctcgccct cgttgggctg cacgaagcgc acgtcgaagt tcgagatggc
     4801 gtcgcccttc gggccgcgct cgacgccgat caggcgcacg tagggcgcgt gcacggcggt
     4861 gtggtccagg gtgaagctct cgacgtcggc catggtgatc ctccagatct ttcagctgtc
     4921 aggatgccac gccgcaccgc ggaggcggca gcggatgacg cccggtgtca gccggcgagc
     4981 cccagcgagg gggcgtgggg cgccagcgag atgagcaggc aggccgtcca gtggcacagg
     5041 aacgccagga cggtgcagac gtggaagatc tcgtggaagc cgaagtggcc gggccacggg
     5101 ttgggcttct tcatcgcgta gatcaccgcg ccgatcgtgt acagcacgcc gcccacgcag
     5161 accagcacca tcatggcgac gttggcgtgc accaggtcga ccatgtacat cacggcggcc
     5221 cagccgaggg cgagatacag cgcgacgtac agccagcgcg gcgcattgat ccagaagacg
     5281 cggaacagga tgcccaggat cgcgccgctc cacacgacga tcagcagcag cgtgcccttg
     5341 ccgggctcca gcgccagcac cgccagcggc gtgtacgtgc ccgcgatcag cagcaggatg
     5401 ttcgcgtggt cgatgcgctt gagcaccatc ttggtcttcg gaccccagtt gaagcggtgg
     5461 tacagcgcgg agttgccgaa cagcagcagc gaggtggcca tgaacaccgc ggcggcccat
     5521 ttgcccgggg cgccgtgcgc gagcacgatc aggacgatgc ccgccgcgat cgcgacgggg
     5581 aaggtgcccg cgtggatcca tccccgccag ctcggtttga tctcgacggc ggcgtccttc
     5641 gccgcggcct cgagaagcgg gagctggggg acatcgggat cgcctgcggc tcgggtgctc
     5701 acgtgctcac tctaggcgcg gccgcattcc gtcagccgta catatcctgc gcgctccggt
     5761 gcctcccggt ctgtggaggt agcgtaggga tgtgagtgca cgcgagagct cggggcgggg
     5821 acccctctat cggctctacg ggaaccgtct tcgacgtcgg atcgacccgg cgaccgtgcc
     5881 gcaccacgtc gcgatgatga tcgacggcaa ccgccgctgg gcccgtcagc tcggctacga
     5941 gtcggcggcc gacggccatc gcgcgggtgc agcgaagatg cgggagttcc tcggctggtg
     6001 cgacgagctc ggcgtggacg tggtctcgct ctacctgctg tccgccgaca acctgaccaa
     6061 gcgggattcg aaggagatcg gcgacctcat cgagatcatc gccgaactcg cggacaccct
     6121 gtcgaagcac ggcgactggc gagtgcagca cgtcggcagg gcagacatcc tgccgccgga
     6181 gctggcgcgc gtgctcgcgg atgccgagga gcgcacccgc gaccacaccg ggctgcacgt
     6241 gaacctcgcg gtcggctacg gcggtcgcaa tgagatcgtg gacgcggtgc gcagcatcat
     6301 cggcaagcac aacgcgtccg gcggcaccct ggaggacctc gcggcccatc tcacccccga
     6361 gatgatcggc gagcacctgt acaccggcgg acagcccgac cccgatctgg tgatccgcac
     6421 cagcggcgag cagcgcctca gcgacttcct gctctggcag agcgcccaca gcgagttcta
     6481 cttcgtcgag gcgctcggcc cggacctccg gcgggtggac ttcctgcgcg ccatccgcga
     6541 cttcgccgac cgcgaccgcc gcttcggtcg ctgacacgtc gcggcgcgcc gacctccgcc
     6601 ggatgcgcaa gactggacgg atgagcgctc tggatgacta cctcgcgtcg ttcacggggg
     6661 agaccggcta tctgaactgg gcggcgttcg gtccgctcgc gccggcggtc cgatccgcga
     6721 tgcacgatgc ggtcgaggtg atgggcggat acagtccacg cgagcatgcg accgtcaccg
     6781 ctcgcaccgg gcgcgccgtc gccggcatcg cggagatgct cggcgcgaag cgggcggagg
     6841 tcgtgctgcg tccgagctcg acggatgccc tgcagcacgc cctgtacggg ctgagcggcg
     6901 cagtgatcgc gggatccggt gagtttccca gcatcagcct cacgctgcag cgcgccgccg
     6961 acgcgtcgtc cggtgcgctc gcgccgcgct ggatcgaccc ggccgacggg cgggtcacga
     7021 cggatgccgt cgcgaccgca ctcgacgacg acgtgacggc ggtggcggtc agccatgtcg
     7081 actaccgcac cggctatcgc gcggacctga cggcgctgcg agagctcatc ggccccgatc
     7141 ggctgctcat cgtggacgcg gtccagtcgt tcggcgtcgt cgacgaggac tggagcaccg
     7201 ccgacgtggt ggccgggcac gggtacaagt ggctgcgttc ggggcggggc accggcttcg
     7261 cgcgcttctc cgaccgcgcc gtggagcgcc tccgtcccgt gctcagcggg atcaccggca
     7321 tcgacgccga cgggccgttc tccg
//
LOCUS       DATMSX010000464         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_16439,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000464 DATMSX010000000
VERSION     DATMSX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16439"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VN041_14810"
     CDS             <1..934
                     /locus_tag="VN041_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWU30352.1"
                     /translation="SAWRDEVQHTLAAPPAVPPVAPVEAREATARHGVAEYMDMVERC
                     RDAIERGDAYQLCLTTRFTVPGTVEPLAAYRRLRTATPAHHGGYIRIGGRHLLSASPE
                     QFLSVGGGIASTSPIKGTRPRAADPVEDARLAEELRTDAKERAENVMIVDLMRNDLSH
                     VGEPGTVRVDRLWEVESYPAVHQLVSTVSVRLREGVTLGDLADAAFPAGSMTGAPKLS
                     AMTILHGLERAPRGVYSGCFGHVGVDGGVDLAMVIRSIVVEPEVTYVGAGGGITWLSK
                     ADAEAAEVAVKARAPLAALGAAVPAEWAGILQSR"
     gene            1034..3526
                     /gene="leuS"
                     /locus_tag="VN041_14815"
     CDS             1034..3526
                     /gene="leuS"
                     /locus_tag="VN041_14815"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWU30353.1"
                     /translation="MSAHDIQAKWQQYWAENETFLAGGEDDDRPRKYILGMFPYPSGD
                     LHMGHAINYLYPDIVSRYWRHRGHNVLNPIGWDSFGLPAENAAIKRGADPREWTYKNI
                     DQHKAGFRGYGMSYDWSRVLHTSDPEYYRWNQWLFLKLYERGLAYRKKSAVNWCPNDQ
                     TVLANEQVVDGRCERCGAEVVKKKLTQWYFRITEYADRLLDDLDALEGHWPHKVLQMQ
                     RNWIGRSTGADVDFRIEGREEPVTVFSTRPDTLHGATFFVVAPDSDLAAELASGSSAE
                     VRMRFQDYLEQVQKQTEIERQSTDRPKTGVFLERFAINPVNGEKLPIWAADYVLADYG
                     HGAVMAVPAHDQRDLDFARAFDLPVKIVVDTTVAGGANEGGDLAELDPSVTGTALVGN
                     GRLVNSGEIDGLDKAEAIATTIERLQAAGTGRAAKNFRLRDWLISRQRFWGTPIPMLH
                     LQDGEVVPVPEDRLPVTLPSVEGLDLRPKGTSPLGAAESWVRTIDPASGEPALRDPDT
                     MDTFVDSSWYFLRFLSPNSDQFAFDPAEAKRWAPVDGYFGGIEHAILHLLYSRFITKV
                     FFDLGLIDFQEPFSNLVNQGMVLMDGSKMSKSKGNLVELSDLLAQYGADSLRLALAFA
                     GPVEDDKEWNGVSLSGAHKFLARVLRVGDETSSPVGSDAAAGDVTLRRITHRLLADAP
                     GLVEQTKFNVLVARLMEVVNGIRKTIDAGAGAADPAVREAAETVAMMIETIAPHTGEE
                     LWARLGHEPSVSAVQWPEADPALLVDDTVTTAVQVNGKVRATIDVPADISEADLEAAA
                     RADEKVQRALEGKEIVRVIVRAPKIVNFAVKG"
BASE COUNT          595 a         1290 c         1330 g          552 t
ORIGIN      
        1 atccgcctgg cgcgacgagg tgcagcacac cctggcggct cctcccgccg tgccgccggt
       61 cgcgccggtc gaagcacggg aggcgaccgc ccggcacggc gttgccgagt acatggacat
      121 ggtggagcgc tgccgcgacg cgatcgagcg cggggacgcc taccagctgt gcctcaccac
      181 ccgcttcact gtgccgggga cagtcgagcc gttggcggcg taccggcgcc tgcgcaccgc
      241 gacgcccgcg caccatggcg ggtacatccg catcggcggg cggcatctgc tcagcgccag
      301 ccccgagcag ttcctctccg tcggcggcgg gatcgcgtcc accagcccga tcaagggcac
      361 ccggccccgc gccgccgatc ccgtggagga cgcacgtctc gccgaggagc tgcggacgga
      421 tgccaaggag cgggccgaga acgtgatgat cgtcgatctc atgcgcaacg atctgtcgca
      481 cgtcggcgag ccgggcaccg tgcgcgtgga ccggctgtgg gaggtggagt cgtaccccgc
      541 cgtgcatcag ctggtcagca cggtgagcgt gcggctgcgg gagggcgtca cgctgggaga
      601 cctcgcggat gccgccttcc cggcgggcag catgacggga gcgccgaagc tgtcggcgat
      661 gacgatcctg cacgggctcg agcgcgctcc gcgaggggtg tactccggat gcttcgggca
      721 tgtcggcgtc gacggcgggg tcgacctggc gatggtcatc cgctcgatcg tggtcgaacc
      781 ggaggtgacg tacgtcggcg ccgggggcgg gatcacctgg ctgtcgaagg cggatgccga
      841 ggcggcggag gtcgccgtca aggcgcgcgc accgctggcg gcgctgggcg cggcagtgcc
      901 cgccgagtgg gcgggcatcc tccagtcgcg ataacctgga atatcccgct tctcgcgggc
      961 tccctcgcat cgtcagattg gtcgttcctt gtccgagcac gcgtccgaaa cccaccccga
     1021 ttccgctgag gcgatgtccg cgcacgacat ccaggccaag tggcagcagt actgggctga
     1081 gaacgagacg ttcctcgccg gcggcgaaga cgacgaccgg ccgcgcaagt acatcctggg
     1141 catgttcccg tacccctccg gcgacctgca catggggcac gcgatcaact acctgtaccc
     1201 cgacatcgtc tcgcgctact ggcggcaccg cgggcacaac gtgctcaacc cgatcggctg
     1261 ggactccttc ggcctgccgg ccgagaacgc cgccatcaag cgcggtgcgg acccgcgcga
     1321 gtggacctac aagaacatcg accagcacaa ggccggcttc cgcggatacg gcatgtcgta
     1381 tgactggtcg cgcgtgctgc acacctccga ccccgagtac taccgctgga accagtggct
     1441 gttcctgaag ctgtacgaac gcggcctggc gtaccgcaag aagagcgccg tgaactggtg
     1501 cccgaacgac cagaccgtgc tggccaatga gcaggtcgtc gacgggcgct gcgagcgctg
     1561 cggcgccgag gtcgtgaaga agaagctcac gcagtggtac ttccggatca ccgaatacgc
     1621 cgaccggctg ctggatgacc tggacgcgct cgaggggcac tggccgcaca aggtgctgca
     1681 gatgcagcgc aactggatcg gccgctccac cggtgctgac gtcgacttcc ggatcgaggg
     1741 ccgcgaggag ccggtgacgg tgttctccac ccgccccgac accctgcacg gagcgacgtt
     1801 cttcgtggtc gcgcccgact ccgacctggc tgcggaactg gcatccggtt cctccgccga
     1861 ggttcgcatg cggttccagg actacctgga gcaggtgcag aagcagaccg agatcgagcg
     1921 ccagtccacc gaccggccca agaccggcgt gttcctggag cggttcgcga tcaacccggt
     1981 caatggcgag aagctgccga tctgggccgc cgactacgtg ctggccgact acggtcacgg
     2041 cgccgtgatg gccgtgcccg cgcacgatca gcgcgacctg gacttcgccc gcgccttcga
     2101 cctgccggtg aagatcgtcg tcgacacgac ggtcgccggc ggcgcgaacg agggcggcga
     2161 tctggccgag ctcgacccgt cggtgaccgg caccgcgctg gtcggcaacg gccggctggt
     2221 gaactcgggc gagatcgacg ggctggacaa ggcggaggcg atcgccacga ccatcgagcg
     2281 gctgcaggcg gccggcacgg gccgtgcggc caagaacttc cgcctgcgcg actggctgat
     2341 ctcgcgccag cgcttctggg gcacgccgat cccgatgctg cacctgcagg acggcgaggt
     2401 cgtcccggtg cccgaggacc ggctgccggt cacgctgccg agcgtggagg gactggacct
     2461 gcggcccaag ggcacctcgc cgctgggtgc ggcggagtcg tgggtgcgca cgatcgatcc
     2521 ggcatcgggc gagcccgcac tgcgcgatcc ggacaccatg gacaccttcg tggacagctc
     2581 ctggtacttc ctgcggttcc tgtcgccgaa cagcgaccag ttcgcgttcg acccggccga
     2641 ggccaagcgc tgggcaccgg tcgacggcta cttcggcggc atcgagcacg cgatcctgca
     2701 cctgctgtac tcgcgcttca tcaccaaggt gttcttcgac ctcgggctga tcgacttcca
     2761 ggagccgttc tccaacctgg tcaaccaggg catggtgctg atggacggct cgaagatgtc
     2821 gaagagcaag ggcaacctgg tcgagctctc cgatctgctg gcgcagtacg gtgccgattc
     2881 gctgcgtctg gctctggcct tcgccggccc ggtggaggac gacaaggagt ggaacggcgt
     2941 ctcgctgtcg ggagcgcaca agttcctcgc ccgtgtcctg cgggtcggcg acgagacctc
     3001 cagcccggtc gggtcggatg ccgccgcggg cgacgtcacg ctgcgccgca tcacgcaccg
     3061 tctgctcgcc gacgcaccgg gcctggtcga gcagaccaag ttcaacgtgc tcgtcgcgcg
     3121 cctgatggag gtggtgaacg gcatccgcaa gacgatcgac gcgggcgcgg gtgctgccga
     3181 ccctgcggtg cgcgaggccg ccgagacggt ggcgatgatg atcgagacca tcgctccgca
     3241 caccggcgag gagctgtggg cgcggctcgg acacgagccg tcggtcagcg ccgtgcagtg
     3301 gccggaggcc gatcccgcgc tcctggtcga cgacaccgtc accaccgccg tgcaggtcaa
     3361 cggcaaggtg cgcgccacga tcgacgtgcc ggccgacatc tccgaggccg acctggaggc
     3421 cgccgcgcgc gccgacgaga aggtgcagcg ggctctggag ggcaaggaga tcgtgcgcgt
     3481 catcgtgcgc gcgccgaaga tcgtgaactt cgccgtcaag ggctgaacac tccgacacga
     3541 cgtccgagag ctcctgcaca tcctcgtgat gcgcaggagt tctccacatc cgtcgcgttc
     3601 gccggcatcc ggcgtccgag cggatgcgta gcgtgacgga gtgcccgcac ccgaacccat
     3661 cgccgaggac gcgccgtccg aagatgccgc accgcgccgg cggctgaggc tgggcatcgg
     3721 ggcggcgatc accctgggcc tggtggtgct gtccgcggcg gtcggct
//
LOCUS       DATMSX010000465         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_139802,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000465 DATMSX010000000
VERSION     DATMSX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139802"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VN041_14820"
     CDS             complement(<1..693)
                     /locus_tag="VN041_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30354.1"
                     /translation="MGISTMTALSPADERAGDVLRLRREIARMQRRRSDDAVLPLHPA
                     LQGLLPERGLQTGAVYSVSPSPSLIFALMSTVSQRGSWCAAVGMPTLGLEAAASFGVE
                     LSRLILVPEPGERWLAVVSALAEVVPLIAVNPGSRVGDADAARLAARLRDRGCTVLAT
                     TGGISASSWPQSEGMISLHDPHWEGLGEGWGLLSDRTVTLTAQTRTTPRPQSLRVQLP
                     GALGSVEAVRVPD"
     gene            796..1086
                     /locus_tag="VN041_14825"
     CDS             796..1086
                     /locus_tag="VN041_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HWU30355.1"
                     /translation="MRIEFESEVTRWSARVDSWFFATLPEDLSLEIRELPAPPRGFGS
                     LRVRARIGLTSWDTSIFFSGTAFVLPLKKQVRDAQGIEEGDVVLVDLDILEV"
     gene            1154..1624
                     /locus_tag="VN041_14830"
     CDS             1154..1624
                     /locus_tag="VN041_14830"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWU30356.1"
                     /translation="MTLHAVAVLLVHLALAGVGLWLVVIDAREHRLPNRIVLPVLGAL
                     VVLMIVEALVTEDAGRMLRALLGGLALGVFYALMHLGSKQGMGGGDVKLAAVIGVVLA
                     WHGWWTLLLGAAAAFVLGALFAIVLMMLKRANRSTRIAFGPWMILGAALAIAAT"
     gene            1679..1894
                     /locus_tag="VN041_14835"
     CDS             1679..1894
                     /locus_tag="VN041_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU30357.1"
                     /translation="MALARLHGGPLDGQIIPLDSADQTSLILPYSETQLTYRREGEAT
                     NTGAHDGPTEVGFRFVESGDDISPHDD"
     gene            1884..2513
                     /locus_tag="VN041_14840"
     CDS             1884..2513
                     /locus_tag="VN041_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HWU30358.1"
                     /translation="MTTDPASEPQRSFEIERKYDADADTALPDWTSVPGVTAVSEGEL
                     RELDARYLDTSDRALAQRGFALRRRTGGPDAGWHIKGPRLGDGRVELGWPLDEDGRMP
                     EAVAETVAQLTSGELVQIARIENSRTAYHLEGDAGVIAEFVDDHVRATDLTTGIQRVW
                     REWEMELGQAAPADEPGRETFFAAVEQAVLAAGGRPPSSGSKLARALGV"
     gene            complement(2609..4006)
                     /gene="lpdA"
                     /locus_tag="VN041_14845"
     CDS             complement(2609..4006)
                     /gene="lpdA"
                     /locus_tag="VN041_14845"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWU30359.1"
                     /translation="MPHYDVVILGAGPGGYVAAVRSAQLGLSTAIIEEKYWGGVCLNV
                     GCIPSKSLLKNAEIAHTFNHKAEFFGISGDVHFDFGAAFDRSRTVANTHVKGIHFLMK
                     KNKVTEYDGRGTFTGPKAISVAKSDGTTEEVTFDNAIIATGSTVRLLPGVQLSENVVT
                     FEEQILTRDLPESIVIVGAGAIGMEFGYVLSNYGVKVTIIEFLDRALPNEDVEVSKEI
                     QKQYKKLGVDILTSTAVKTVTDNGSSVHVTYETRDGQPGEITAGKVLMSVGFAPNVDG
                     YGLENTGVKLTERGAIDIDDHMRTNVEGIYAIGDVTAKLQLAHVAEAQAVVAAETIGK
                     AETQTLGDYRMMPRATFCQPQVASFGLTEEQARAEGREIKVAKFPFSANGKANGLGEP
                     VGFVKLIADAEHLELIGGHLIGPDVSELLPELTLAQKWDLTALEAARNVHTHPTLSEA
                     LQEGFHGLAGHMINL"
     gene            4183..4611
                     /locus_tag="VN041_14850"
     CDS             4183..4611
                     /locus_tag="VN041_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWU30360.1"
                     /translation="MRHDVTQTFGHESDLSFVPFGADLTEAESAAIAALPAGSALLLV
                     RAGALAGARYLLDADVTTVGRHPEADIFFDDVTVSRRHAEITRAGGAFEIIDQRSLNG
                     TYVNGERVDRSELANGYELRIGKFRMNFFASPIDLAAAND"
     gene            4611..>4964
                     /locus_tag="VN041_14855"
     CDS             4611..>4964
                     /locus_tag="VN041_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWU30361.1"
                     /translation="MAAAGAARERTAPTGLLSIGQVLARLTPEFSQLTSSKLRFLEVQ
                     GIVTPSRTESGYRKFSAHDVERLRTALTLQRDHYLPLSVIREQLDEAEAKGQGWADIV
                     PRSITPPARRHRRDEL"
BASE COUNT          738 a         1726 c         1724 g          776 t
ORIGIN      
        1 atccgggacc cgcaccgcct cgacgctgcc cagtgctccg ggcagctgca cgcgcaggct
       61 ctgcggccgc ggcgtcgtgc gggtctgcgc ggtgagggtc acggtgcggt ccgacagcag
      121 accccatccc tcgccgagcc cctcccagtg cgggtcgtgc aggctgatca tgccctcgct
      181 ctgcggccag gacgaggcgg atatcccacc cgtggtggcc agcaccgtgc atccgcggtc
      241 gcgcagccgg gcggccagcc gggcggcatc cgcatccccg accctcgacc cgggattgac
      301 cgcgatcagc ggcacgacct cggccagagc cgagacgacc gccagccatc tctccccggg
      361 ctcggggacc aggatcagcc gggacagctc gacgccgaag gatgcggcgg cctcgagacc
      421 cagcgtgggc atgcccaccg cggcgcacca gctgccgcgc tgcgagaccg tgctcatcag
      481 ggcgaagatc aggctcggcg agggcgacac cgagtagacc gcgccggtct gcagcccccg
      541 ctcggggagc agcccctgca gcgcgggatg cagcgggagc accgcgtcat cgctgcgccg
      601 gcgctgcatc cgcgcgatct cgcggcgcag tcgcagcaca tcccccgcgc gttcgtcggc
      661 gggggagagc gccgtcatcg tactgatccc catgcatcca tcctcgaaga aagattcgaa
      721 caattcaagt ccatttcgaa gcgtagatcg cacatccgac atcgcagagc cggtcgtctc
      781 gtctacgctg gatccatgcg gatcgagttc gaaagcgagg tcacgcgctg gagtgcgcgg
      841 gtggactcgt ggttcttcgc gacgctgccc gaggacctct cgctcgagat ccgcgagctg
      901 cccgcaccgc cgcgcggctt cggctcgctg cgcgtccggg cgaggatcgg cctgaccagc
      961 tgggacacgt cgatcttctt cagcgggacg gcattcgtgc tgccgctgaa gaagcaggtg
     1021 cgcgatgcgc aggggatcga ggagggcgac gtcgtcctcg tcgatctcga catcctcgag
     1081 gtctgaggcc cttcgccgcc gggttgtcca cagggcatcg gcgggcctcc gcggattcgc
     1141 ctagccttcc agcatgaccc tccacgccgt cgccgtgctg ctcgtgcacc tcgcgctggc
     1201 gggcgtgggg ctgtggcttg tcgtcatcga cgcccgcgag caccggctgc ccaaccgcat
     1261 cgtgctgccc gtgctcggcg cactggtcgt gctgatgatc gtcgaggcac tggtcacgga
     1321 ggatgccgga cggatgctgc gcgctctgct cggcgggctc gcgctcggcg tcttctacgc
     1381 cctgatgcac ctcggatcga agcaggggat gggcggcggt gatgtgaagc tcgccgccgt
     1441 gatcggggtg gtgctcgcct ggcacggctg gtggacgctg ctgctgggag cggcagcggc
     1501 gttcgtgctg ggagccctgt tcgcgatcgt gctcatgatg ctcaagcggg cgaaccgctc
     1561 cacccgcatc gccttcgggc cgtggatgat cctcggcgcc gccctcgcga tcgcggcgac
     1621 ctgacgacgg gcgggccggc ctcgccgcgt tgtccccgca gggggctact ctgagcacat
     1681 ggcactcgca cgactccacg gcggcccgct cgacgggcag atcatcccgc tcgacagcgc
     1741 ggaccagacc tcgctgatcc tgccctacag cgagacccag ctgacgtatc gccgcgaggg
     1801 cgaggcgacc aacacgggcg cccatgacgg gcccaccgag gtggggttcc ggttcgtcga
     1861 atccggtgac gacatctccc cccatgacga ctgatcccgc atcggagccg cagcgctcct
     1921 tcgagatcga gcgcaagtac gacgcggacg ccgacacggc actgccggac tggaccagcg
     1981 tccccggcgt gaccgcggtg agcgagggtg agctgcgcga gctcgacgcc cgctacctcg
     2041 acacctctga ccgcgctctc gcgcagcgcg gtttcgcgct gcgccggcgc accgggggac
     2101 cggatgccgg ctggcacatc aagggcccgc gcctgggcga tggccgggtg gagctcggct
     2161 ggccgctgga cgaggacggc cggatgccgg aggccgtggc cgagacggtc gcacagttga
     2221 cctcgggcga gctggtgcag atcgcccgca tcgagaacag caggacggcc taccacctgg
     2281 agggcgacgc cggtgtgatc gccgaattcg tcgacgacca tgtgcgcgcc acggacctga
     2341 cgacgggcat ccagcgcgtg tggcgggagt gggagatgga gctcggccag gcggcacccg
     2401 ccgacgagcc cggccgagag acgttcttcg cggccgtcga gcaggcggtc ctcgctgccg
     2461 gcgggcgtcc gccttcctcc ggctccaagc tcgcccgcgc actcggggtc tgagaccgac
     2521 acggagtcga agccgatccg gctccgcccg cggaacgacg agagcccggg tcgtgctcac
     2581 gcacgcccgg gctctcgtcg agtggaactc agaggttgat catgtgaccc gcgaggccgt
     2641 ggaagccctc ctgcagggcc tccgacaggg tcgggtgggt gtgcacgttg cgtgcggcct
     2701 cgagagcggt gaggtcccac ttctgcgcga gcgtcagctc ggggagaagc tcggagacgt
     2761 ccggtccgat caggtggccg ccgatgagct cgaggtgctc ggcatccgcg atcagcttga
     2821 cgaagccgac cggctcgccc aggccgttgg ccttgccgtt ggcgctgaac gggaacttcg
     2881 cgaccttgat ctcccggccc tcggcgcgcg cctgctcctc ggtgagaccg aagctggcga
     2941 cctgcggctg gcagaacgtg gcgcggggca tcatccggta gtcgcccagc gtctgggtct
     3001 cggccttgcc gatggtctcg gcggccacga cggcctgcgc ctcggccacg tgggccagct
     3061 gcagcttggc ggtcacgtcg ccgatggcgt agatgccctc gacgttcgtg cgcatgtggt
     3121 cgtcgatgtc gatcgcgccg cgctcggtga gcttgacgcc ggtgttctcc aggccgtagc
     3181 cgtccacgtt cggtgcgaag ccgacggaca tgagcacctt gccggcggtg atctcgccgg
     3241 gctggccgtc gcgcgtctcg taggtgacgt gcacggacga gccgttgtcg gtgacggtct
     3301 tcaccgcggt ggaggtgagg atgtcgacgc cgagcttctt gtactgcttc tggatctcct
     3361 tggagacctc gacgtcctcg ttcgggaggg cgcggtcgag gaactcgatg atcgtgacct
     3421 tcacaccgta gttcgacagc acgtagccga actccatgcc gatggcaccg gcgccgacga
     3481 tgacgatgga ctcgggcagg tcgcgggtga ggatctgctc ctcgaaggtg acgacgttct
     3541 cgctcagctg cacgcccggc agcaggcgca cggtcgaacc ggtggcgatg atggcgttgt
     3601 cgaaggtgac ctcttcggtc gtgccgtccg acttggcgac cgagatcgcc ttcgggccgg
     3661 tgaaggtgcc gcggccgtcg tactcggtga ccttgttctt cttcatcagg aagtggatgc
     3721 ccttgacgtg ggtgttcgcc accgtacggc tgcggtcgaa cgccgcaccg aagtcgaagt
     3781 gcacgtcacc cgagatgccg aagaactcgg ccttgtggtt gaacgtgtgc gcgatctccg
     3841 cgttcttcag gagcgacttc gaggggatgc agcccacgtt gaggcagaca cctccccagt
     3901 acttctcctc gatgatggcg gtggacaggc cgagctgcgc gctgcggacg gcggcgacgt
     3961 agccgccagg gcccgcgccg aggatgacga catcgtaatg tggcatgtca tcaagcctat
     4021 cgtctgcacc gcgcgcggga gcggccgtgc aacccgatcg ctacgcagat cgccctcccg
     4081 agcacccgat cgcatcagat aggctgaagg accggaggag ggagccccgt gacagacgac
     4141 agcacgatcg gcgccggagg cgcgatccac cgcaacggcg aagtgcggca cgacgtgacg
     4201 cagacattcg gccatgagtc cgacctgtcc ttcgtgccgt tcggcgcgga tctgacggag
     4261 gcggagagcg cggccatcgc cgccctgccc gcaggctccg cgctgctgct ggtgcgcgcc
     4321 ggtgccctgg ccggcgcccg ctaccttctc gacgccgacg tgacgaccgt cggccggcac
     4381 cccgaggccg acatcttctt cgacgacgtg accgtgtcgc gccgtcacgc cgagatcacc
     4441 cgtgcgggcg gcgccttcga gatcatcgac cagcgctccc tgaacggcac ctacgtgaac
     4501 ggcgagcggg tcgaccgcag cgagctggcc aacggctacg agctgcgcat cggcaagttc
     4561 cggatgaact tcttcgcatc cccgatcgat ctcgcggcgg cgaacgactg atggcggcgg
     4621 ccggcgccgc ccgggagcgc accgcgccca ctgggctcct cagcatcggc caggtcctgg
     4681 cccggctcac ccccgagttc tcccagttga cctcgagcaa gctccgcttc ctcgaggtgc
     4741 agggcatcgt caccccgtcc cgcacggagt ccgggtaccg gaagttctcg gcccacgacg
     4801 tcgaacgcct gcgcaccgcg ctcacgctgc agcgtgacca ctacctcccg ctcagcgtga
     4861 tccgcgagca gctcgacgag gccgaggcca aggggcaggg ttgggccgac atcgtgcccc
     4921 gctcgatcac cccgcccgcg cgccggcacc ggcgtgacga gctg
//
LOCUS       DATMSX010000466         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_45628,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000466 DATMSX010000000
VERSION     DATMSX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45628"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VN041_14860"
     CDS             <1..222
                     /locus_tag="VN041_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWU30362.1"
                     /translation="GGQAEELPDGLRITPVPLTGAVWPAHHDHRMATTGALIGLRVPG
                     VEIDDIGTTAKTLPEFTMLWERMLTGATA"
     gene            219..1262
                     /gene="rsgA"
                     /locus_tag="VN041_14865"
     CDS             219..1262
                     /gene="rsgA"
                     /locus_tag="VN041_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWU30363.1"
                     /translation="MSWLGDDDEDSDFEEFDESSIRSRPNPKANRPRTKRRPAHEDAE
                     IGRILGVDRGRYSVLIGEDRPDERTVTSARARELRRTPIVTGDRVRVVGDTSGDEGTL
                     SRIVGIVDRTSLLRRSADDTDQVERVIVANADQMLIVVAAADPEPRARLVDRYLVAAL
                     DAGIRPLLVVTKTDLADPSDFLSHFEGLDLTVFTSARGQMPVDEIGAALIGHSTVFVG
                     HSGVGKSTLVNALVPGAGRATGHVNEVTGRGRHTSSSTVSLRYVSDAGRGWVIDTPGV
                     RSFGLGHVDRANILAAYTELAEIAEDCPRGCTHLPDAEDCALNLAAADGTLGEAGRSR
                     LESLQRLLETFGG"
     gene            1272..1748
                     /gene="bcp"
                     /locus_tag="VN041_14870"
     CDS             1272..1748
                     /gene="bcp"
                     /locus_tag="VN041_14870"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWU30364.1"
                     /translation="MTTTRLEPGTPAPDFTLLDQDGTSVSLSDLRGKKVILYFYPAAM
                     TPGCTTQACDFRDSISSLQGAGYTVVGVSRDEPAKLKKFQERDGLTFELLSDPDHAVH
                     TAYGVWGEKMNYGKVVEGVIRSTFVIDEQGVIELAQYNVKATGHVARLRKALGVDA"
     gene            complement(1761..2171)
                     /locus_tag="VN041_14875"
     CDS             complement(1761..2171)
                     /locus_tag="VN041_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30365.1"
                     /translation="MRRSGPAAIAAVILGLEGVALAVVGIIELFALGAGDASSAASGI
                     ALIGLTLIGAAALIAFGFGVLRRASWARSGGIVFQVLAIALSLAALTIRPTPWTFVLV
                     LGIVGVVGLVTLIATVRREGADDPRLQERPSDDA"
     gene            2378..2626
                     /locus_tag="VN041_14880"
     CDS             2378..2626
                     /locus_tag="VN041_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HWU30366.1"
                     /translation="MDWRDKAACLTVDPELFFPVGNTGPAVDQIEKAKAVCATCTVTE
                     ICLQYALESGQDSGVWGGLSEDERRALKRRAARARRAG"
     gene            complement(2689..4161)
                     /locus_tag="VN041_14885"
     CDS             complement(2689..4161)
                     /locus_tag="VN041_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HWU30367.1"
                     /translation="MSTLSDLVHAQGRLNDADVEWLHRLAGDGQLLADLASADIVMWI
                     ETADGSFMAVAHSRPSGAATLFYRDIVGEKVRPQWRTQVQSAFESGEIVDSSSPDWFE
                     ETPTRVRAVPIKRKHDGGAIVGVITRHTNLGEARMPSRQQISFDECANDLFRMIASGE
                     FPDLAAPTAPRRGAPRASDGLIRLDVDGITTFASPNALSAFNRMGFDDELEGESLAEV
                     TTRIVPPSRQVDESLPVVVTGRAPWRTDMEARGVTVSLRAIPLKDHGTRIGAIVLCRD
                     VSELRHQEQELITKDATIREIHHRVKNNLQTVASLLRIQARRTRSDDARDALTQAMRR
                     VESIAVVHDTLASGLSQTVDFDEVFDRVLKLVAEVAAAPNTRARTQLEGRFGVLPSEY
                     ATPLALALTEVVTNAVEHGLAGQEGIVRIEATRTSELLTVTVTDTGHGLPEGRVGQGL
                     GTQIVRTLIQGELSGTIEWKGTEGEGTQVTIAIPMRWIAA"
     gene            complement(4214..4360)
                     /locus_tag="VN041_14890"
     CDS             complement(4214..4360)
                     /locus_tag="VN041_14890"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HWU30368.1"
                     /translation="MLSIRVTEPVDGIVEAVVIVAGPGRTRAVAVRLEGLDGRWRASS
                     FAVL"
BASE COUNT          693 a         1554 c         1490 g          640 t
ORIGIN      
        1 ggcggccagg ccgaggagct gcccgacggc ctgcgcatca cccccgtgcc tctgaccggc
       61 gcggtgtggc ccgcccatca cgaccaccgg atggcgacca cgggcgcgct gatcggcctg
      121 cgtgttcccg gcgtcgagat cgacgacatc ggcaccaccg cgaagaccct tcccgagttc
      181 acgatgctct gggagcggat gctgacgggc gccaccgcgt gagctggctg ggcgacgacg
      241 acgaggactc cgacttcgag gagttcgacg agagcagcat ccggtcgcgt ccgaacccga
      301 aggccaaccg accccgcacc aagcgccgcc ctgcgcatga ggatgccgag atcgggcgca
      361 tccttggcgt cgaccgcggc agatacagcg tgctgatcgg cgaggaccgt cccgatgaac
      421 gcaccgtgac gtcggcccgc gcgcgcgagc tgcgccgcac gccgatcgtc acgggcgatc
      481 gggtcagggt cgtcggcgac acctcgggcg atgaagggac gctgtcccgc atcgtgggca
      541 tcgtcgatcg cacctcgctg ctgcgccgca gcgccgacga caccgatcag gtggagcggg
      601 tcatcgtcgc caacgccgac cagatgctga tcgtggtggc cgctgcagac cccgagccgc
      661 gcgcgcgtct ggtcgaccgc tacctcgtgg cggccctgga tgccggcatc cgcccgctcc
      721 tggtcgtgac caagaccgat ctcgccgacc cctcggactt cctgtcgcat ttcgagggcc
      781 tcgacctcac cgtgttcacc agcgcgcgcg ggcagatgcc cgtggacgag atcggcgcgg
      841 cgctgatcgg ccactccacg gtgttcgtcg gccactccgg ggtaggcaaa tcgacgctgg
      901 tgaacgcgct ggtcccggga gcgggacgcg ccaccgggca cgtcaacgag gtcaccggcc
      961 gcggacggca cacgtcctcg tcgacggtct cgctgcgcta cgtctcggat gcggggcgcg
     1021 gctgggtgat cgacacaccc ggcgtgcgct cgttcgggct cgggcatgtg gaccgtgcca
     1081 acatcctcgc cgcctacacg gaactcgcgg agatcgccga ggactgcccg cgcggctgca
     1141 cgcacctgcc cgacgccgag gactgcgcac tgaacctggc cgcggcggac gggacgctgg
     1201 gcgaagcggg acgctctcgc ctggagtcgc tgcagcgtct gctggagacg ttcggcggat
     1261 aggctggtgg cgtgaccacg acgcgcctgg aacccggtac ccccgccccc gacttcaccc
     1321 tgctcgatca ggacgggacc agcgtctcgc tctccgatct gcgcgggaag aaggtcatcc
     1381 tgtacttcta ccccgcggcg atgacgcccg gatgcaccac gcaggcctgc gatttccgcg
     1441 acagcatctc ctcgctgcag ggtgccggtt acacagtcgt cggcgtctcg cgggacgagc
     1501 ccgccaagct gaagaagttc caggagcgcg atggcctcac cttcgagctg ctcagcgatc
     1561 ctgaccacgc cgtgcacacc gcctacggcg tgtggggcga gaagatgaac tacggcaagg
     1621 tcgtcgaggg tgtcatccgc tcgaccttcg tcatcgacga gcagggcgtc atcgagctcg
     1681 ctcagtacaa cgtcaaggcc accggccacg tggcccggtt gcgcaaggcg ctcggcgtgg
     1741 acgcctgagc gcgggcgtcc tcacgcgtcg tcggacgggc gctcctgcag tcggggatcg
     1801 tcggcgccct ccctgcgcac cgtcgcgatc agcgtcacca gccccacgac gcccacgatc
     1861 ccgaggacga gcacgaacgt ccagggcgtg ggccggatgg tgagcgccgc aagcgacagc
     1921 gcgatggcga gcacctggaa gacgatgccg cccgaacgcg cccaggacgc acggcgcagc
     1981 acgccgaagc cgaaggcgat cagcgcggcg gcgccgatca gggtcaatcc gatcagtgcg
     2041 atgccgctcg ccgccgacga cgcatcgccc gcaccgaggg cgaacagctc gatgatgccg
     2101 accaccgcga gtgcgacgcc ctcgagtccc aggatgacgg ctgcgatggc ggcggggccg
     2161 gatcggcgca cgggaactcc tcagggatga atgtcacaaa aacccttgat ttcgcacatt
     2221 ccatgtaaca gaatagacaa agccatgtgc tcccacatcg gagtggggcg ggtgcaacac
     2281 ccgcatgacc agggtagctg actcccgatc cgcttcccga actcgagtgc cgcgtctgcg
     2341 gccaccacac cgaaaatcta tctctgagga gccatccatg gattggcgcg acaaagcggc
     2401 ctgccttacc gtcgaccccg aactgttctt cccggtcggc aacaccggcc ccgcagtcga
     2461 ccagatcgag aaggccaagg ccgtctgcgc cacctgcacc gtcaccgaga tctgcctgca
     2521 gtacgccctg gagtccggcc aggactcggg cgtgtggggc ggcctctccg aggacgagcg
     2581 tcgcgcactg aagcgccgtg ccgcacgcgc acgccgcgcc ggctgaactc ccccgcaccc
     2641 gacgaagaag cgccggtccc catcgggacc ggcgcttctc tcatgacgtc aggcggcgat
     2701 ccaccgcatg gggatcgcga tggtcacctg cgtgccctcg ccctcggtgc ccttccactc
     2761 gatcgtgccg ctgagctcgc cctggatcag ggtgcgcacg atctgcgtac cgagtccctg
     2821 gccgacccgc ccctcgggaa ggccgtgccc cgtgtcggtc accgtcacgg tcagcagctc
     2881 cgacgtgcgc gtcgcctcga tgcggacgat gccctcctga cccgcgaggc cgtgctcgac
     2941 cgcgttggtg acgacctcgg tcagcgcgag cgcgaggggt gtggcgtact cgctgggcag
     3001 cacgccgaag cgtccttcca gctgcgtgcg cgcccgggtg ttcggagcag ccgcgacctc
     3061 ggcgacgagc ttgagcacgc ggtcgaagac ctcgtcgaag tcgacggtct gggacagccc
     3121 gctggcgagg gtgtcgtgca cgaccgcgat ggactcgaca cggcgcatgg cctgggtgag
     3181 cgcgtccctg gcgtcgtcgg agcgcgtcct gcgcgcctgg atgcgcagca gcgaggcgac
     3241 cgtctgcagg ttgttcttca cacggtgatg gatctcgcgg atcgtcgcat ccttggtgat
     3301 gagctcctgc tcctggtggc gcagctccga cacgtcacgg cacagcacga tcgcgccgat
     3361 ccgggtgccg tggtccttca gcgggatcgc gcgcagcgac accgtcacgc cgcgggcctc
     3421 catgtcggtg cgccaggggg cgcggccggt cacgaccacg gggagcgact cgtccacctg
     3481 gcgggagggc ggcacgatcc gcgtggtcac ctcggccagc gactcgccct cgagttcgtc
     3541 gtcgaatccc atccggttga acgccgacag ggcattgggg ctcgcgaagg tggtgatgcc
     3601 gtcgacgtcg agccggatca ggccgtcgga tgcacggggg gcgccacgcc ggggcgcggt
     3661 cggcgccgcg agatccggga actcgccgga tgcgatcatg cggaacaggt cgttcgcgca
     3721 ttcgtcgaag ctgatctgct ggcgggacgg catccgcgcc tcgcccaggt tggtgtgccg
     3781 ggtgatcacg cccacgatgg cgccgccgtc gtgcttgcgc ttgatgggca ccgcgcgcac
     3841 gcgcgtggga gtctcctcga accagtccgg tgacgaggag tccacgatct ccccggactc
     3901 gaatgccgac tgcacctgcg tgcgccactg cggacgcacc ttctccccca cgatgtcgcg
     3961 gtagaagagg gtcgcggctc cactgggccg ggagtgcgcg acggccatga aggacccgtc
     4021 ggcggtctcg atccacatca cgatgtccgc ggaggcgagg tcggccagaa gctgcccgtc
     4081 gcccgcgagg cgatgcagcc attcgacatc cgcgtcgttc aggcggccct gggcgtgtac
     4141 gagatcactg agcgttgaca ctccctcagc ctactggggg cgttcggcgc cgaatgcgtc
     4201 cgccgcgcac gcgtcacagc acggcgaacg agctcgcccg ccatctgccg tccaggccct
     4261 ccagccgcac cgcgaccgca cgcgtccggc cgggacctgc gacgatgacg accgcctcga
     4321 cgatgccgtc caccggctcg gtgacgcgga tcgagaggat ctggaagttc ggccgcg
//
LOCUS       DATMSX010000467         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101980,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000467 DATMSX010000000
VERSION     DATMSX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101980"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VN041_14895"
     CDS             <1..201
                     /locus_tag="VN041_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30369.1"
                     /translation="IWSAAAVLMPLAGDIHPSWPELERALTVTARPDSVDADPGPERQ
                     LPERESRAADPGTAESDPKETR"
     gene            198..1067
                     /locus_tag="VN041_14900"
     CDS             198..1067
                     /locus_tag="VN041_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWU30370.1"
                     /translation="MTLVSARDLMRASAAAGTGLGAFNVIHLETAEALASASERAQLP
                     VILQISQNCADYHGGLEPIGWATLAIARRAKTPVAVHLDHAERPELVDEAIELGFGSV
                     MFDGGKLDYEENVELTADIAGRARAAGVYIEAELGEVGGKDGAHAPGVRTDPDEARAF
                     VEATGVDSLAVAVGSSHAMLDRSAALDIDLIGRLKSALRGIGRNGGDVPLVLHGSSGV
                     ADDVIVRAVQAGMTKINVSTHLNGFFTRAIRETLTADEKLVDSRKYVAPGREAVAEEA
                     ARMLRLFALAERA"
     gene            1073..1849
                     /locus_tag="VN041_14905"
     CDS             1073..1849
                     /locus_tag="VN041_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HWU30371.1"
                     /translation="MNRAARLTAILDLLAAQGEVSVEQLVDRFGASAATTRRDLDSLA
                     ERRLLTRTHGGAVAQSVAYELPIRYKSHLGTGAKEQIAVLAADLVSPGDVVGLSGGTT
                     TTAIATALAARDDLGAEGLTVVTNAVNIAAQLAMRPEFKVVVTGGAIHSRSFELVGPF
                     AEQLLGGIRLDIAFIGVNGLSAAEGATTHDEAEAAVNRMMAERAARAVIVADASKLGR
                     VSFAHVGGPALFPLVLTDAGVADVDRTALADAGFEVRTAG"
     gene            complement(2083..2583)
                     /locus_tag="VN041_14910"
     CDS             complement(2083..2583)
                     /locus_tag="VN041_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU30372.1"
                     /translation="MPASDSSTAHRTAMADAGLDAVDRTIIAELSRNGRLSNTELAAR
                     AGIAESTCLKRVRALQANGVIVGFHAEISPAAIGLHLEALITIRLHAHARGDLRRFQA
                     YVEDLPATQRVYFLAGDRDFLVHVAVPDAAALRELVSDTLSLRPEVASTSTSLIFAHA
                     AGSQVL"
     gene            2756..3979
                     /locus_tag="VN041_14915"
     CDS             2756..3979
                     /locus_tag="VN041_14915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWU30373.1"
                     /translation="MTRTIVVGAGMVGLATAWHLQERGVEVTVIDRVGVAAGSSWGNA
                     GWLTPGKTIPLADTGLWTYGPKALLDPDAALHVPFRVDAGLWSFLAQFAAHGTNRAWD
                     RTMASLTPIDKMALECFDELLDGGVDSWTREGPFVIGFKQEADSKGFLHEIEGVVRHG
                     QEVPFERLENPRELAPVLSDAVTHVYRLDGQRFFEPAPFVEALGEAVVARGAELITGL
                     EVIDVSSTRRPVVALSNGERMEADSVVIATGAWMPKLARRLGVRTRVQAGRGYSFTVA
                     TAQPQEHPVYFPSQRIACTPYQGRFRIAGTMEFRAPDEPFQPRRIQAIIAQAREMLQG
                     IDFEERQDEWMGSRPVTPDGLPLVGATQAPNVYVAGGHGMWGIVLGPATGKLLAEQIT
                     TGRIHPAIEPFDPLR"
BASE COUNT          604 a         1457 c         1441 g          603 t
ORIGIN      
        1 atctggtcgg ccgccgccgt gctgatgcct ctcgccggcg acatccaccc ctcctggccc
       61 gagctcgagc gcgccctcac cgtcaccgca cgacccgact ccgtggacgc ggacccgggc
      121 cccgagcgac agcttcccga acgagaatcc cgcgccgccg accccggcac cgcagaatcc
      181 gacccgaagg aaacccgatg accctcgtct cagcacgcga cctgatgcgc gcgtccgccg
      241 ccgccggcac cggcctcggc gccttcaacg tgatccacct cgagaccgcc gaggcgctcg
      301 cctcggcatc cgagcgggca cagctgcccg tgatcctgca gatctcgcag aactgcgccg
      361 actaccacgg cgggctcgag ccgatcggct gggcgaccct cgccatcgcc cgcagggcga
      421 agaccccggt cgccgtgcac ctcgaccacg ccgagcgccc cgagctcgtc gacgaggcca
      481 tcgaactcgg cttcggatcg gtgatgttcg acggcggcaa gctcgactac gaggagaacg
      541 tcgagctcac ggccgacatc gccggccggg ctcgcgccgc aggtgtctac atcgaagccg
      601 agctgggtga ggtcggcggc aaggacgggg ctcacgcacc cggagtccgc accgaccccg
      661 acgaggcgcg cgccttcgtc gaggccaccg gcgtcgactc gctggccgtc gcggtcggat
      721 cctcgcacgc gatgctcgac cgctcggctg cgctcgacat cgacctgatc ggacgcctga
      781 agagcgcact gcgcggcatc ggccgcaacg gcggagacgt gccgctcgtg ctgcacggat
      841 cgtccggcgt cgccgacgac gtgatcgtgc gcgcggtgca ggccgggatg acgaagatca
      901 acgtctccac gcacctcaac ggattcttca cccgcgcgat ccgcgagacg ctcaccgccg
      961 acgagaagct cgtcgactcc cgcaagtacg tcgctcccgg ccgcgaggcc gtcgccgagg
     1021 aggccgcacg gatgctgcgc ctgttcgctc tcgccgagcg ggcgtgatga gcatgaaccg
     1081 cgccgcccgc ctgaccgcga tcctcgacct gctcgccgca cagggcgagg tgtcggtcga
     1141 gcagctcgtc gaccgcttcg gcgcatccgc cgcgacgacc cgtcgcgatc tggacagtct
     1201 cgctgagcgg cgcctgctca cccgcacgca cggcggtgcc gtggcgcagt cggtcgccta
     1261 cgagctgccg atccggtaca agagccacct cggcacgggc gcgaaggagc agatcgcggt
     1321 tctggcggct gacctcgtgt cgcccggcga cgtggtcggg ctctccggcg ggaccacgac
     1381 gaccgcgatc gccaccgcgc tcgccgcgcg cgacgatctc ggtgccgagg ggctgacggt
     1441 cgtgacgaat gcggtgaaca tcgctgccca gctcgcgatg cggcccgaat tcaaggtggt
     1501 cgtcacgggc ggtgcgatcc actcgcgcag cttcgagctg gtcggcccgt tcgctgagca
     1561 gctgctcgga ggcatccgcc tggacatcgc cttcatcggc gtgaacgggc tgtcggccgc
     1621 ggagggcgcg acaacccacg acgaggccga ggccgccgtg aaccggatga tggccgagcg
     1681 cgccgcgcgg gccgtgatcg tggcggatgc gtccaagctc ggacgcgtgt cgttcgcgca
     1741 cgtgggcggc ccggcgctgt ttccgctggt gctcacggat gccggcgtcg ccgatgtcga
     1801 tcgcacggcg ctggcggatg ccggcttcga ggtgcgcacc gcgggctgac cccgcgtcgg
     1861 caaccgccgc gcatccaggt cgcgatggat gccgcttcgt aggggcttgc cgtgtccagg
     1921 tcgcgatgga tgccgcttga tcgacccttc ggcggcaacc agtgcgacct ggagctgccg
     1981 gagcgacacc gtagcggcaa ccaatgcgac ctgaagctgc cgaagtgatc ccgcgtcggc
     2041 gaccagtgcg acctggaagg tcttgtggat cagcgcgcag cgctacagca cctgcgatcc
     2101 ggcggcgtgc gcgaagatca ggctcgtcga ggtcgacgcc acctccggcc gaaggctgag
     2161 cgtgtccgac accagctcgc gcagcgcggc agcatccgga accgcgacgt gcaccaggaa
     2221 gtcccggtca ccggcgagga agtacacccg ctgcgtggcg ggcagatcct ccacgtaggc
     2281 ctggaagcgg cgcaggtcgc cgcgggcgtg cgcgtgcagc cggatcgtga tcagcgcctc
     2341 gagatgcagg ccgatcgcgg ccggcgagat ctcggcgtgg aagcccacga tcaccccgtt
     2401 cgcctgcagc gcgcgcacgc gcttgagaca cgtggactcg gcgatcccgg cgcgcgccgc
     2461 gagctcggtg ttggacagcc gcccgttgcg gctgagctcg gcgatgatcg tgcggtcgac
     2521 agcgtcgagc ccggcgtccg ccatcgctgt gcggtgggcc gtcgaagaat cggatgccgg
     2581 catgctcgcg ttcccattcg gagtgaagat tctgcggtgt gcgcatcgat cttacgcagg
     2641 aactttcgac cttggccacg ctgatgagcg agatctgcat aatcgtggat gagttggggt
     2701 tcgtcgtgcc tcgactcttc tttccgctta acgacgggtt cttcaggagg acgcaatgac
     2761 gcgcacgatc gtcgtcgggg ccggaatggt cggtctcgcc acggcatggc acctgcagga
     2821 gcgcggggtc gaggtgacgg tcatcgaccg ggtcggtgtc gccgccggct cgtcgtgggg
     2881 caacgccggc tggctgacgc cgggcaagac catcccgctg gccgacaccg gcctgtggac
     2941 ctacgggccg aaggcactgc tcgacccgga tgcggccctg cacgtgccgt tccgcgtgga
     3001 cgcgggcctg tggtcgttcc tggcgcagtt cgccgcgcac ggcacgaaca gggcgtggga
     3061 ccgcaccatg gcatccctca cgccgatcga caagatggcg ctcgagtgct tcgacgagct
     3121 gctcgacggc ggtgtggatt cgtggacccg cgaggggccg ttcgtgatcg gcttcaagca
     3181 ggaggccgac tcgaagggct tcctgcacga gatcgagggc gtcgtccggc acgggcagga
     3241 ggtgccgttc gagcggctgg agaatccgcg cgagctcgcg ccggtgctgt cggacgccgt
     3301 cacgcacgtc taccggctgg acggccagcg cttcttcgag ccggcgccct tcgtcgaggc
     3361 gctcggcgag gcggtcgtcg ctcgcggcgc cgagctgatc accggactgg aggtcatcga
     3421 cgtgtcctcc acccgtcgcc cggtggtggc gctgtcgaac ggcgagcgga tggaggccga
     3481 ctcggtcgtc atcgcgaccg gcgcctggat gccgaagctc gcccgcaggc tcggggtgcg
     3541 cacgcgcgtg caggccggcc gcggctactc gttcactgtc gcgactgcgc agccgcagga
     3601 gcatccggtg tacttcccgt cgcagcgcat cgcctgcacc ccttaccagg ggcgcttccg
     3661 catcgccggc accatggagt tccgcgcacc ggacgagccg ttccagccgc gtcgcatcca
     3721 ggcgatcatc gcccaggccc gggagatgct tcagggcatc gacttcgagg agcgccagga
     3781 cgagtggatg ggctcgcgcc cggtcacacc cgacggcctg ccgctggtcg gcgcgactca
     3841 ggctccgaac gtgtacgtcg ccggtggcca cggcatgtgg ggcatcgtgc tcggccccgc
     3901 gaccggcaag ctgctggccg agcagatcac gacgggccgc atccaccccg cgatcgagcc
     3961 cttcgacccg ctgcgctgag cggcgtccgg gccctcccgg gcacggcgtc ggcagactgg
     4021 atcagaatcg cctcggaagc gcgattcccc gaggcgattc tgttccggcc cggtgcgcgg
     4081 ggcggagcgg atgccggcgc tcgac
//
LOCUS       DATMSX010000468         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_101985,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000468 DATMSX010000000
VERSION     DATMSX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101985"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1386
                     /locus_tag="VN041_14920"
     CDS             166..1386
                     /locus_tag="VN041_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWU30374.1"
                     /translation="MVDSSTRIAIIGGGMGGLTAAIALTKIAGAQVTVFEQASRLGEV
                     GAGVTLAPNAARVLDRLGVLDGIRAVGAIPDGHGVYLDGLGNLVTDAAWEDSAQRYQN
                     IGMYRPDLIDGLAKHVDPDSIRLGHRLKSVQTVDAGVRVEFENGTEEIFDAVVGADGI
                     HSVVRGAVGQHPEPVYSGYLVYRGVMDASRLPDDWPMISQVWMANRRHFMAYPLQQRR
                     LFNFVAGVPSDRPLHGAWSGPAEVSELAAEFSGEDWDPRLQHFISLIDKTFWWGLFDH
                     DPLTNWSRGPITLLGDAAHTMLPHQGQGVSQAIEDSVALATFLAAADTIADIPEAFRR
                     YTAVRMQRTAILQNGSRRAGAMFDAQYEFQNLTKRDADIRVGRDFRRSAVFDYDAHKV
                     AEEALARFRRVQVA"
     gene            1383..2003
                     /locus_tag="VN041_14925"
     CDS             1383..2003
                     /locus_tag="VN041_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWU30375.1"
                     /translation="MTSISIIGSGKMSGAIAEVAARAGAGIQIIKRSATSTSAARPGL
                     EYGVMGDELTGDLVVLAVPYGAYEGILEHYRDQLSEKVVIDISNPIDFSTYDRLRSPA
                     DSSTAAELAKLLPEGSAVVKAFNVNLGDTLVSGTNGTTRTTVLYAGDYADAKMSVAAL
                     IEAAGMRAVDVGPLTRARELEAMGFLQIIMAALGRTRYESGFTLLE"
     gene            complement(2106..3035)
                     /locus_tag="VN041_14930"
     CDS             complement(2106..3035)
                     /locus_tag="VN041_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU30376.1"
                     /translation="MDSSHDTFGAFVEELAAAMRDDEVATLTGDELATRVGFSRFHFD
                     RVIRSAAGEPPAALRRRILLERAAFRILTTDASLIDVAVDAGYGSHEAFTRAFAKAFG
                     APPAEWRQHPGQIRIPAANDVHFHPPGSIRLPSRGAVSDMDLLTRMVEHHVWLTGEMI
                     RVAQRLEPDALERPIEIDIDDDPQTIRSLLARLTGQLAMWNAVMAGRGYDIAQERDQS
                     LGELRRVLDVEGPAFLAHVREVIAEDRLDETFVDAGGEPVQMISYGGNIAHILTFAAY
                     RRTLVCLALAAHGHDELGWGDPRHWVIGPEPTR"
     gene            complement(3130..3402)
                     /locus_tag="VN041_14935"
     CDS             complement(3130..3402)
                     /locus_tag="VN041_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWU30377.1"
                     /translation="MSAAPDDEIRKVVNRLKRARGQLTAVIDAMETDADCRDVVIQLA
                     AVGKAIDRAGFAVIGTALRHCVADGEDARDGAPTMEELQKLFLTLA"
BASE COUNT          552 a         1210 c         1217 g          526 t
ORIGIN      
        1 gcctgcgcga tcgccgcggc atcgcactcg gccccgacaa cgaggacgag caggccgtcg
       61 gagccacggt cttcgcatcc gccgcgcccg caggcgaggt cgccggccag ctcgcgggaa
      121 cgcgatgagg cccgccccgg cacgcacgtc agtcaaggag aagcaatggt agacagcagc
      181 accaggatcg cgatcatcgg cggagggatg gggggcctca ccgccgccat cgctctcacg
      241 aagatcgccg gagcacaggt gacggtgttc gagcaggcga gccgtctggg cgaggtcggc
      301 gcaggggtca cgctcgcgcc gaacgccgcg cgagtgctcg acaggctcgg tgtgctcgac
      361 gggatccgag cggtgggcgc catccccgac gggcacggcg tgtacctcga cggtctgggc
      421 aacctcgtca ccgatgccgc ctgggaggac tcggcccaga ggtatcagaa catcgggatg
      481 taccgacccg acctcatcga cggcctcgcg aagcacgtcg accccgattc gatccgcctc
      541 ggccacaggc tgaagtcggt gcagacggtg gatgcgggcg tgcgggtcga gttcgagaac
      601 gggacggagg agatcttcga cgcggtcgtc ggcgccgacg gcatccactc ggtcgtgcgg
      661 ggcgccgtgg gccagcatcc cgaacccgtc tactcggggt acctcgtcta ccgcggagtc
      721 atggacgcct cgcgcctgcc ggacgactgg cccatgatca gccaggtgtg gatggcgaat
      781 cgccggcact tcatggccta cccgctgcag cagcgcaggc tgttcaactt cgtcgccggc
      841 gtcccgagcg accggccgct ccacggcgcc tggtcgggcc cggccgaggt cagcgaactc
      901 gccgccgagt tctcgggaga ggactgggac ccgcggctgc agcacttcat cagcctgatc
      961 gacaagacgt tctggtgggg tctgttcgac catgatccgc tcaccaactg gtcgcgaggg
     1021 cccatcacgc tgctcggcga cgcggcccac acgatgctgc cgcaccaggg ccagggcgtg
     1081 agccaggcga tcgaggacag cgtcgcgctc gccacgttcc tcgcggcggc cgacacgatc
     1141 gccgacatcc ccgaggcgtt ccgacgctac accgcggtgc gcatgcagcg caccgcgatc
     1201 ctgcagaacg gctcgcgccg tgccggtgcg atgttcgacg cgcagtacga attccagaac
     1261 ctcaccaagc gcgacgccga catccgcgtc gggcgcgact tccgccgcag cgcggtgttc
     1321 gactacgacg cgcacaaggt cgccgaagag gcactcgcac gattcagaag ggttcaggtc
     1381 gcatgacttc gatcagcatc atcggcagcg gcaagatgag cggggcgatc gccgaggtcg
     1441 ccgcgcgcgc gggggccggc atccagatca tcaagcgcag cgcgaccagc acgtcggccg
     1501 cgcgtcctgg cctggagtac ggcgtcatgg gcgacgagct cacgggcgac ctcgtggtgc
     1561 tcgccgtccc ctacggggcg tacgagggca tcctggagca ctaccgcgac cagctcagtg
     1621 agaaggtcgt gatcgacatc agcaacccga tcgacttctc cacctacgac cggctgcgct
     1681 cccccgcgga ctcgtccacc gcggccgagc tggcgaaact gctgcccgag ggatcggcgg
     1741 tggtgaaggc gttcaacgtc aacctcggcg acaccctggt cagcggcacc aacggcacga
     1801 cccgcaccac ggtgctgtac gcgggagact atgcggatgc caagatgagc gtcgccgcgc
     1861 tgatcgaggc cgccgggatg cgcgccgtcg acgtcggacc gctgacccgc gcccgtgagc
     1921 tcgaggcgat gggcttcctg cagatcatca tggcggcgct cggccggacg cgctacgagt
     1981 cggggttcac gctgctggag tgatcgcacg cacgcacgtt cgggaggagt tctcaccttc
     2041 gggaggagtc ctgggacagg atgctcctcc cgttgcggag ttctcctccc gagcgtgtgg
     2101 tgcgatcagc gagtcggctc cggcccgatc acccagtgcc gcgggtcgcc ccagccgagt
     2161 tcgtcgtgtc cgtgcgcggc caacgccagg cacacgaggg tacggcgata tgcggcgaac
     2221 gtcaggatgt gggcgatgtt tccaccgtac gagatcatct gcacgggctc gccgccggca
     2281 tcgacgaagg tctcatccag cctgtcctcg gcgatgacct cacgcacgtg cgcgaggaat
     2341 gccggccctt cgacgtcgag cacgcgccgc agctctccga gggactgatc ccgctcctgc
     2401 gcgatgtcgt agccgcgccc ggccatcacg gcgttccaca tcgccagctg cccagtcagc
     2461 cgcgccagca gtgaccggat ggtctgcggg tcgtcgtcga tgtcgatctc gatgggcctc
     2521 tccagggcgt ccggttcgag ccgctgggcg acgcggatca tctccccggt cagccagacg
     2581 tgatgctcga ccatgcgtgt cagaaggtcc atgtcgctca ccgctcctct cgaggggaga
     2641 cgaatgctcc ccggtggatg gaaatggacg tcgttcgcgg cggggatgcg gatctgtccc
     2701 ggatgctgcc gccattcggc gggcggcgcc ccgaacgctt tcgcgaacgc gcgggtgaat
     2761 gcctcgtgcg agccgtaccc ggcgtcgacg gcgacgtcga tgagggaggc atccgtggtc
     2821 aggatccgga acgccgcgcg ctcgagcagg atgcgcctgc ggagcgccgc cggcggctcg
     2881 cccgcggccg agcggatcac ccgatcgaag tggaatcggg agaatcccac ccgcgtcgcg
     2941 agctcgtcgc cggtgagcgt cgccacctcg tcgtcgcgca tggccgcagc cagctcctcg
     3001 acgaaggcgc cgaacgtgtc gtgtgacgag tccatggaag aatcatcgcc tcggcgcggg
     3061 accgcgcgct tgatcctgtt tgcgggatgc cggagttccg cggctgtcct cggggtgccg
     3121 gccgcgagct caggccaggg tgaggaacag cttctgcagc tcctccatcg tcggcgcccc
     3181 gtcccgggcg tcctccccgt ccgcgacgca gtggcgcagt gcggtgccga tcaccgcgaa
     3241 accggcgcga tcgatggcct tgccgactgc cgcgagctgg atgaccacat cgcggcagtc
     3301 ggcatcggtc tccatcgcgt cgatcacggc ggtgagctgg ccgcgcgccc gcttgaggcg
     3361 gttcacgacc ttgcggatct catcgtcggg tgcggcgctc atgcgggctc cttcacgctc
     3421 gggcgggacg cggcgagcat ccgcgttccg gcggaccagg tgagataccc gccgtcgagg
     3481 ttgcgcgccg gccggccgtg ctgac
//
LOCUS       DATMSX010000469         5997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_64854,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000469 DATMSX010000000
VERSION     DATMSX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64854"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN041_14940"
     CDS             <1..207
                     /locus_tag="VN041_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWU30378.1"
                     /translation="GNVSVQFQGNRPRFSARRAEAEAALSQALGGASVSVSATTTDGL
                     GFPGRGEGISVTAVALVLPRGGAG"
     gene            204..713
                     /locus_tag="VN041_14945"
     CDS             204..713
                     /locus_tag="VN041_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30379.1"
                     /translation="MNELTFRQPRRTLIMVFGLLYLVVVLTAMIVNFDALAEIVNGTA
                     AGAGAKATGMIVGALVFVPLIALGALATQKVRLRQDAETLNVRIGRSEKHLRRRDIVG
                     YTVNEPRVGSIRLRAEDGGVLQDLHPRMQEHAAVLAFIDGDDRYVEAGRTTAFRGRVN
                     VVSYALRGH"
     gene            768..2177
                     /gene="cysS"
                     /locus_tag="VN041_14950"
     CDS             768..2177
                     /gene="cysS"
                     /locus_tag="VN041_14950"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWU30380.1"
                     /translation="MTIRLYDTRAQELRDFVPLDPSNVTMYVCGPTVQSGPHIGHVRA
                     ALSFDILRRWLELRFGHVTFVRNVTDIDDKILANATEVEPWWALAYRMEKEFTEAYAA
                     VGILPPTYEPRATGFIPQMHDLIAALIERGHAYPAADGSGDVYFDVRSWPAYGELTRQ
                     SVDAMEAAADADPRGKRNPQDFALWKGAKPEEQADAVWASPWGAGRPGWHIECSAMSK
                     RYLGAEFDIHGGGLDLRFPHHENELAQSTAAGDGFARYWVHNGLVTVGGQKMSKSLGN
                     FTLAKDFLDGVDPVVARYALAAAHYRSSLDLTESTLAEAEAALGRIRGFIERAVRVTG
                     GVGWMAQQSLPQAFSAAMDDDLGVPQALAVLHETVRAGNAALDKGDADGVRTALQAVK
                     AMTQVLGVDPLSSERRTGGSDAAASALDTLVQTMIAQRAQARADKDWAAADRIRDAIA
                     AAGITLEDTPDGTHWSIDG"
     gene            2170..3171
                     /gene="rlmB"
                     /locus_tag="VN041_14955"
     CDS             2170..3171
                     /gene="rlmB"
                     /locus_tag="VN041_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWU30381.1"
                     /translation="MAKPGRPGAGNSKKKGASKGTGGLGRKALEGRGPTPKAEDRSWH
                     VAGKRKAAAERYAAAGGKGKPGQKPQSSLNRQSRGSKPADDTETVTGRNSVLEALRTK
                     IPATAFYIAQRVEMDDRVKEMLAITRHREIPVLEVTRQELDRMAGFDGVHQGVALKVP
                     PYEYAHPQDLLEQVIDRGQTPLFVALDGITDPRNLGAIIRSTAAFGGHGVILPQRRSA
                     SVNSAAWKTSAGAAARLPVALASNLTSMLKEFKKQGVFVLGMDGDGDVMLPQLELADR
                     PVVIVIGSEGKGLSRLVAETCDQIVSIPISAATESLNAGIAASVALYQVSTVRGGRE"
     gene            3229..3870
                     /locus_tag="VN041_14960"
     CDS             3229..3870
                     /locus_tag="VN041_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWU30382.1"
                     /translation="MARIVVIGGTGYAGAHFVREGVSRGHDVVAVARKAPADPIEGAT
                     YVQGSALDVAGLGDVLDGADAVISTISPRGDMADKALDALSALIAKLTGTSTRLGVVG
                     GAGGSLVAPGGPRLFDQGFPEEYKHEAQVGIDSLELLQESGQGVDWFFVHPAEVFGPW
                     AEGERTGAYRDGGDVIVRDADGNSTISGADFAVAFLDEIENPKHRRERFAVGY"
     gene            complement(3965..4882)
                     /locus_tag="VN041_14965"
     CDS             complement(3965..4882)
                     /locus_tag="VN041_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU30383.1"
                     /translation="MILVTGGLGAIGAHTALALVESGADVVVTGHRRTDVPSFLAGRV
                     TVEVLDVTDREAFLALARRHPITDIVHLAGSIPGSDTVDFFRHDLTGLLNALDAALAW
                     KVRRFAVASSIGVYIGRDERPWREELALPAVEVPLSIIAFKKAVEPIVTDALHGSGIQ
                     PVILRIGSTWGPLMDPETPFSAVPPIVGAVQRGEEPAPLDADGGGDFGYAPDVGRAIA
                     LLMTTETLDHTIYNVSSGSTFTNRELADAIAQALPGSRPRLIGGGDGTAQHPYLDISR
                     LSATTGFVPEFDVGKAVAHYVAWRRENPR"
     gene            complement(4879..5472)
                     /locus_tag="VN041_14970"
     CDS             complement(4879..5472)
                     /locus_tag="VN041_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU30384.1"
                     /translation="MSRVITALSVSADGFITGRDPRPGQGLGDGGVLFDWYTSGDVAS
                     GLFPGFTLTAASARFFDAVGGRVGASLAGHTTYSDSEDFAGGAPHPTAPLVVLSHHDL
                     APASERQHIVTTGLADAVSLAKRLAGDKDVALMGGVITTEALKAGLVDELVLHVVPVL
                     LGAGRRYFHELPEHVGLDIVQVVPAPDVTHIHYEVRR"
     gene            complement(5621..>5997)
                     /locus_tag="VN041_14975"
     CDS             complement(5621..>5997)
                     /locus_tag="VN041_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30385.1"
                     /translation="WRPVDVRSAADRLALSESQLRRRCLRVVGLSPKVLQRTLRFQGF
                     LALAQAGPAAPLPHGTRSIAGLAAVAGYADQAHLSRECVRLTGVTPAQLLHGGTGGCA
                     CGHDHSASYLPFLRVRPAGTNH"
BASE COUNT          930 a         2069 c         2155 g          843 t
ORIGIN      
        1 ggcaacgtgt cggtgcagtt ccagggcaac cgcccgcgct tcagcgcccg tcgagccgag
       61 gcggaggccg cgctctcgca ggccctgggc ggagcatccg tctcggtctc cgcgacgacc
      121 accgacggcc tcggcttccc cggccgtggc gagggcatct cggtcaccgc ggtcgcgctg
      181 gtgctgcccc gagggggcgc cggatgaacg agctcacttt ccgccagccc cggcgcacac
      241 tgatcatggt gttcgggctc ctgtacctcg tcgtcgtcct gacggccatg atcgtgaact
      301 tcgatgcgct ggccgagatc gtgaacggca ccgccgccgg cgcgggggcg aaggccacag
      361 gcatgatcgt cggggcgctc gtcttcgtgc cgctgatcgc cctcggagca ctggcgacac
      421 agaaggtgcg gctgcgccag gacgccgaga cgctgaacgt gaggatcggc cgaagcgaga
      481 agcatctgcg acgccgggac atcgtcggat acacggtgaa cgagcctcgt gtcggcagca
      541 tccggctccg ggccgaggac ggcggcgtgc tgcaggacct ccacccccgg atgcaggagc
      601 acgcggctgt gctcgcgttc atcgacggag acgacagata tgtcgaagcc ggacggacca
      661 cggccttccg gggcagggtg aacgtcgtct cctacgcgct gcggggtcac tgagcgaaat
      721 cgcgcaacgc ccaggtgcgt ggaccacggc cccggtagtc ttgtgcggtg acgatccgcc
      781 tctacgacac ccgcgcgcag gaactgcgcg acttcgttcc gctcgacccc tcgaacgtga
      841 cgatgtacgt gtgcgggccg accgtccagt cggggcccca catcgggcat gtccgcgcgg
      901 cgctgagctt cgacatcctg cgccgctggc tcgagctgcg cttcgggcac gtgaccttcg
      961 tgcgcaacgt caccgacatc gacgacaaga tcctggcgaa cgccaccgag gtcgagccct
     1021 ggtgggcgct cgcctaccgg atggagaagg agttcaccga ggcgtatgcc gccgtcggca
     1081 tccttccgcc cacctacgag ccgcgcgcca cgggcttcat cccgcagatg catgacctga
     1141 tcgccgcgct catcgagcgg ggccacgcct atcccgccgc cgacggctcg ggcgacgtct
     1201 acttcgacgt gcgctcgtgg ccggcatacg gcgagctcac gcggcagtcc gtcgacgcca
     1261 tggaggccgc agccgacgcc gacccgcgcg gcaagcgcaa ccctcaggac ttcgcgctct
     1321 ggaagggcgc gaagccggag gagcaggcgg atgccgtgtg ggcctcgccc tggggagccg
     1381 ggcgcccggg gtggcacatc gagtgctccg ccatgtcgaa gcgctatctg ggcgccgagt
     1441 tcgacatcca cggcggcggc ctcgacctgc gcttcccgca ccacgagaac gagctcgcgc
     1501 agtccaccgc agccggcgac ggcttcgcgc gatactgggt gcacaacggc ctggtgaccg
     1561 tgggcgggca gaagatgtcg aagtcgctgg gcaacttcac gctcgcgaag gacttcctcg
     1621 acggcgtcga cccggtcgtg gcgcgctacg cgctcgcggc cgcgcactac cggtcgagcc
     1681 tggatctcac ggagtcgact ctggccgagg ccgaggcggc gctcggacgc atccgcgggt
     1741 tcatcgagcg tgccgtccgg gtcaccggcg gcgtcggctg gatggcccag cagtcgctcc
     1801 cgcaggcgtt cagcgccgcg atggacgacg atctgggcgt gccgcaggcg ctggccgtcc
     1861 tgcacgagac cgtgcgcgcg ggcaacgcgg cgctggacaa gggcgatgcc gacggagtgc
     1921 gcactgcact ccaggctgtg aaggcgatga cccaggttct gggcgtcgac cctctgtcga
     1981 gcgagcggag gacagggggc tcggatgctg cggcatccgc tctggacacg ctcgtgcaga
     2041 cgatgatcgc gcagcgtgcg caggcgcgcg ccgacaagga ctgggccgca gccgatcgca
     2101 tccgcgatgc gatcgcggcg gcaggaatca ctctggagga cactccggac ggaacacatt
     2161 ggagtatcga tggctaagcc tggacgcccc ggagcgggca acagcaagaa gaagggcgcc
     2221 tccaagggca ccggcggtct gggccgcaag gctctggagg gccgcggacc cacacccaag
     2281 gcggaggacc gcagctggca cgtcgccggc aagcgcaagg ccgccgcgga gcgctacgcc
     2341 gcggccggcg gaaagggcaa gccgggccag aagccgcagt cgagcctgaa ccgtcagtcg
     2401 cgcggctcga agcccgcaga cgacaccgag accgtcaccg ggcgcaactc ggtgctcgag
     2461 gcgctgcgca ccaagatccc cgccacggcg ttctacatcg cccagcgcgt ggagatggac
     2521 gaccgcgtca aggagatgct ggcgatcacg cggcaccgcg agatccccgt gctcgaggtg
     2581 acccgccagg agctcgaccg gatggccggc ttcgacggcg tgcaccaggg cgtggcgctg
     2641 aaggtgccgc cgtacgagta cgcgcatcct caggacctgc tggagcaggt catcgaccgc
     2701 ggccagacgc cgctgttcgt ggcgctcgac ggcatcaccg acccccgcaa cctgggagcc
     2761 atcatccgct ccacggccgc cttcggcggg cacggcgtca tcctgccgca gcgccgctcg
     2821 gcgagcgtga actccgccgc ctggaagacg agtgccggag ccgcagcccg tcttcccgtc
     2881 gcgctcgcgt cgaacctgac gtccatgctg aaggagttca agaagcaggg cgtgttcgtg
     2941 ctcggcatgg acggcgacgg tgacgtcatg ctcccgcagc tcgagctggc cgaccgcccc
     3001 gtggtgatcg tcatcggatc cgagggcaag ggcctctcgc gcctcgtggc cgagacctgc
     3061 gaccagatcg tgtcgatccc gatctcggcg gccaccgagt cgctgaacgc cggtatcgcg
     3121 gcatccgtcg cgctgtacca ggtttcgacg gttcgcggcg gacgggaata gcgcgggagg
     3181 ccggagcctt gaccctgacg gactcattcg tcagcggaag gaacgcacat ggctcgcatc
     3241 gtcgtcatcg gaggaaccgg ctacgccgga gcgcacttcg tccgtgaggg ggtgagccgg
     3301 gggcacgacg tggtcgctgt cgcccgcaag gcgccagccg acccgatcga gggtgcgacc
     3361 tacgttcagg gttcggcact cgacgtcgcc ggcctcggtg acgtgctcga cggtgcggat
     3421 gccgtgatct cgacgatctc gccccgcggc gacatggcgg acaaggcgct cgacgcgctg
     3481 agcgcgctga tcgcgaagct gaccggcaca tcgacccgcc tcggcgtcgt cggcggcgcg
     3541 ggcggcagcc tggtcgcgcc cggcggcccg cggctgttcg atcagggctt cccggaggag
     3601 tacaagcacg aggcgcaggt cggcatcgac tcgctcgagc tgctgcagga gtccggtcag
     3661 ggcgtggact ggttcttcgt gcacccggca gaggtcttcg gcccgtgggc cgagggcgag
     3721 cgcaccgggg cgtaccgcga cggaggcgac gtgatcgtgc gcgacgccga cggcaactcg
     3781 accatctcgg gcgccgactt cgcggtcgcg ttcctcgacg agatcgagaa cccgaagcac
     3841 cgccgcgagc gcttcgccgt cggctactga tcccgaaccg ccggaggggc acgggcccgt
     3901 ccggcggctg tgatcgtctt cgatcagccg aggtcggggc gctccgcatg gcgtgcggcg
     3961 agagtcaccg cgggttctcc cgccgccatg cgacatagtg ggccacggcc ttcccgacgt
     4021 cgaactccgg gacgaagccc gtggtcgcgc tcagccgcga gatgtcgaga tacggatgct
     4081 gcgcggtgcc gtctccgccc ccgatcaggc gcggacgcga accgggcagc gcttgagcga
     4141 tcgcgtcggc gagctcgcgg ttcgtgaacg tcgatccgct cgagacgttg tagatggtgt
     4201 ggtcgagcgt ctccgtcgtc atcagcaggg cgatcgcgcg gcccacgtcg ggggcgtacc
     4261 cgaagtcgcc gccgccgtcg gcgtccaggg gtgcgggctc ctccccgcgc tgcacggcgc
     4321 ccacgatcgg cgggacggcg ctgaacggcg tctcgggatc catcagcggg ccccaggtgc
     4381 tgccgatccg caggatcacc ggctggatgc cgctgccgtg cagcgcatcg gtgacgatcg
     4441 gctccacggc cttcttgaaa gcgatgatcg acagcggcac ctcgacggcc gggagggcga
     4501 gttcctcgcg ccagggccgc tcgtcccgtc cgatgtagac gccgatgctg ctggcgacgg
     4561 cgaacctgcg cactttccag gccagagccg cgtccagggc attgagcagt ccggtcaggt
     4621 cgtgccggaa gaagtccacg gtgtcggacc ccgggatgct tcccgccaga tgcacgatgt
     4681 cggtgatggg atgccggcgc gcgagcgcaa ggaaggcctc ccggtcggtg acgtcgagga
     4741 cctcgacggt cacgcggcct gccaggaagg acggaacgtc ggtcctgcgg tggccggtca
     4801 cgacgacgtc cgcgccggac tcgaccaggg ccaacgccgt gtgcgccccg atcgccccca
     4861 gaccgccggt gacgaggatc atcggcggac ctcgtagtgg atgtgggtga catcgggagc
     4921 gggcacgacc tggacgatgt ccaggccgac gtgctcggga agctcgtgga agtagcgccg
     4981 cccggcgccc agcagcaccg gaaccacgtg cagcaccagc tcgtccacca ggcccgcctt
     5041 cagcgcctca gtggtgatga caccgcccat gagcgccacg tccttgtctc ctgcgagacg
     5101 cttcgcgagg cttacagcat cggcgagtcc ggtggtcacg atgtgctgac gctccgaggc
     5161 gggggcgagg tcgtggtgac tcagcacgac gagcggcgcg gtgggatgcg gtgcgccacc
     5221 tgcgaagtcc tcggagtccg agtaggtcgt gtgcccggcc aggctggcgc cgacccggcc
     5281 gccgaccgcg tcgaagaagc gggcgctggc agcggtcagg gtgaagccgg ggaacagtcc
     5341 gctcgccacg tcaccgctgg tgtaccagtc gaagaggacg ccgccgtcgc cgagcccctg
     5401 tcccgggcga ggatcacgac ccgtgatgaa cccgtcggcc gagacggaca gtgcggtgat
     5461 gactctgctc atgtgcttcc ctctgctcga tctctgttcg gcacgtcggt gcgtcggatg
     5521 ctcggtccac gccgtgctgc gagtgccgct cggcccgaat ctacggcccc tcccggcgcc
     5581 gcggattgaa cgaatcgtgc atgagcggtc ccgccgcgcc tcagtggttc gtcccggccg
     5641 gccgcacccg caggaaggga aggtacgacg cggagtgatc gtgtccgcag gcgcagccgc
     5701 cggtgccgcc gtgcagcagc tgtgccgggg tcacaccggt cagccgcacg cactcgcgac
     5761 tcaagtgcgc ctggtccgcg tagcctgcga cggcggcgag gccggcgatg ctccgggtcc
     5821 cgtgggggag cggagcggcc gggcccgcct gggcgagcgc gaggaacccc tggaatcgca
     5881 gagtgcgctg cagcaccttc gggctcagcc cgacgacccg aaggcaccgg cgacgcagtt
     5941 ggctctcgga gagcgcgagc cgatcggccg cggagcgcac gtcgaccggc cgccacg
//
LOCUS       DATMSX010000470         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_26102,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000470 DATMSX010000000
VERSION     DATMSX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26102"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VN041_14980"
     CDS             <1..579
                     /locus_tag="VN041_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30386.1"
                     /translation="PDRTLVATAHLLASIAPGPRSLMWSWAMPQGDRSGITEKLRAYG
                     AEHGIPELTQGEVAFPADTGEDIQAWVADLAHAIAGAAVEITGLSPYYSAPAGGARAV
                     LLLDAPIEPLTVAAAVTALPRILSGLTLSDPRASVWDLGRLAGWRLEWADEAFSAATL
                     TDATGSATFRFDEYARITGIEGSLGGATTPAP"
     gene            complement(684..1160)
                     /locus_tag="VN041_14985"
     CDS             complement(684..1160)
                     /locus_tag="VN041_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HWU30387.1"
                     /translation="MTAQVEPGRFTAASDRADITVFLIGMRANRWWRVGSVVRTASRM
                     GPMLKHLMTHPDAGLLGFHQWLGRTTVLLSYWESPEHLQRFASDRNAPHLAAWREFVR
                     TSQGSGDVGVWHETYQVPFADLEVVYNDMPPFGLAAATTRVPVGAGSRTARQRLGR"
     gene            1225..1782
                     /locus_tag="VN041_14990"
     CDS             1225..1782
                     /locus_tag="VN041_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30388.1"
                     /translation="MARPTVYDDDLRTRLLEETAEIIDRDGPDRVVLREIAHAAGTST
                     SAIYTLFGGKTELLAAVIEHAFRSFGDAQLTAEPQGLRALGEAYRAWSLAHPALFRLM
                     FGDRMPAEHAAVEAVAVQSLAPLVRTVSGIRPGPRETAQRDAVIIWSQVHGAVALELA
                     QAAPAGVDWDAVYAGLLDVVERSLA"
     gene            complement(1885..2766)
                     /locus_tag="VN041_14995"
     CDS             complement(1885..2766)
                     /locus_tag="VN041_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HWU30389.1"
                     /translation="MTAKVLDGKAAAAAIKDELTERVAALRERGVVPGIATVLVGADP
                     ASQLYVGMKHKQSEAIGMNSIQRELPADATQEQVEALIDELNADPACHGYIVQLPLPK
                     HLDTDAILERIDPAKDADGLHPTNLGRLVLNVNGPIHTPLPCTPRGVIELLLRNDYDL
                     NGKHVVVVGRGVTIGRPMGLLLTRRVINATVTQVHTGTPDMTPYLREADVIVAGAGVK
                     HLITASDVKPGAAVLDVGVTRETDAETGKSKVYGDVHPDVAEVAGFISPNPGGVGPMT
                     VALLMTNVVEAAERSLA"
     gene            complement(2763..4037)
                     /gene="glyA"
                     /locus_tag="VN041_15000"
     CDS             complement(2763..4037)
                     /gene="glyA"
                     /locus_tag="VN041_15000"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWU30390.1"
                     /translation="MTDRYFDAPLAEVDPEIAEVLERELNRQRTYLEMIASENFVPVS
                     VLQSQGSVLTNKYAEGYPGRRYYGGCEEVDVAEELAIARAKALFGSEFANVQPHSGAS
                     ANAAVLHAIARPGDTLLGLSLDQGGHLTHGMKINFSGRLYNIVAYGVNPETSTIDMDE
                     VRALALEHKPKVIIAGWSAYPRTLDFAAFRAIADEVGALLWVDMAHFAGLVAAGLHPN
                     PVPHAHVVSSTVHKTIGGPRSGFILTNDADIAKKINSAVFPGQQGGPLMHVIAAKATA
                     FKLAATPEFKERQERVLRGAAIIAERLTQQDVKDAGIAVRSGGTDVHLVLVDLRDAEI
                     DGKQAEDLLHEIHITVNRNAVPNDPRPPMVTSGLRIGTPALATRGFGDAEFTEVADII
                     AVALQPGADIEALRTRVDALATAFPLYPGLQQ"
     regulatory      complement(4075..4156)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            4229..5749
                     /locus_tag="VN041_15005"
     CDS             4229..5749
                     /locus_tag="VN041_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HWU30391.1"
                     /translation="MVLPDLLPLVPAPVSVVIGEGRMPLADPLRIIGDDAAADQLAAA
                     IGHRTGIRPVRVAAEARPGDVVVRIDPDAAAEAYALTIGEIVELTGSADGLLHGIRTL
                     MQLLADEGDGRWAWRRATIADAPRFAYRGVMLDVARHFFPVDEVEKVIDRIAELKFNH
                     LHLHLSDDQGWRIHIDSWPLLSQLSSTVSATGDGRATGGGCYSKDDYRAIVSYAASRG
                     ITIVPEIDMPGHTHAIGIAYPELVEAPVMNEHLLDDAQRLGQALPEAGVPYTGWGVGH
                     SSLRIHEERTYEFVRDVLAELAELTPGPYLHIGGDESLGTSPADFASFIERATGIVQE
                     LGKTPVAWHEAGAAEVAPGTVGQYWGLLAPDPSHAEHTAHFVERGGRVILSPSDAAYL
                     DMKYDHDFPLGLAWAGTVDLHRAYDWQPTALLDIPADAVAGVEAPLWTETVESLEEAD
                     QLMFPRIAAIAEIAWSASDPTRDWNDFRERLAAQAPAWRADGIRFHPVPEIDWSDR"
     gene            5746..>5985
                     /locus_tag="VN041_15010"
     CDS             5746..>5985
                     /locus_tag="VN041_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30392.1"
                     /translation="MTPTTDFRGSRVIHSARIVDGDGSTTENGWVRFEGGVVIARGTG
                     ADWTDADDVIDASAIGGADAVLTPGFIDIHGHGGAG"
BASE COUNT          850 a         2126 c         2100 g          909 t
ORIGIN      
        1 cccgaccgca cgctggtcgc gaccgcgcac ctgctcgcgt cgatcgcacc ggggccgcgg
       61 tcactgatgt ggtcgtgggc gatgccgcag ggcgatcgct ccggcatcac cgagaagctg
      121 cgcgcgtacg gcgccgagca cgggatcccc gagctgacgc agggcgaggt ggccttcccg
      181 gccgacacgg gtgaagacat tcaggcgtgg gtggccgatc tggctcatgc gatcgcggga
      241 gccgccgtgg agatcaccgg actctctccg tactactcgg ccccggccgg cggcgcccgc
      301 gccgtgctgc tgctggacgc cccgatcgag ccgctcacgg tcgccgctgc ggtcacggca
      361 ctcccccgca tcctctccgg cctcacgctc tccgacccgc gcgcatcggt gtgggacctc
      421 gggcgcctcg cgggatggcg gctcgagtgg gcggacgagg cgttctcggc tgcgacgctg
      481 acggatgcca ccggctcggc gaccttccgc ttcgacgagt acgcgcgcat cacgggcatc
      541 gagggctcac tgggcggcgc cacgactccg gctccctgac gagcttccaa ggcgctcccg
      601 gcgtggccgc ccctgctcag ttacctcgcc cctgctcatt tgcgtagctc agcggggggg
      661 cgaggagcac cccagtcgga cagtcagcgc ccgagacgtt ggcgcgccgt gcgactcccc
      721 gcaccgacgg gcacgcgcgt tgtcgcggcg gcgagcccga acggcggcat gtcgttgtac
      781 acgacctcga ggtcggcgaa cggcacctga taggtctcgt gccagacgcc gacgtcgccg
      841 gagccctgcg acgtgcggac gaactcgcgc cacgcggcga ggtgcggcgc attgcggtcc
      901 gacgcgaagc gctgcaggtg ctccgggctc tcccagtacg agagcagcac ggtggtgcgc
      961 ccgagccact gatggaagcc gagcagcccg gcatcgggat gggtcatcag atgcttcagc
     1021 atcggcccca tccgcgaagc ggttcgcacg acgctgccga cacgccacca gcgattcgcc
     1081 cgcatcccga tcaggaacac cgtgatgtcc gcgcgatcgg atgccgccgt gaagcgcccc
     1141 ggttccacct gagcggtcat gatctctccc gtcgaagctt tacgtaacgt tgttatgaaa
     1201 cgacgttacg ctacgatcgt gaccatggca agacccaccg tctacgacga cgacctgcgc
     1261 acgagactgc tggaggagac cgccgagatc atcgaccgcg acggccccga tcgcgtcgtc
     1321 ctgcgtgaga tcgctcacgc ggcaggcacc tcgacgtcag cgatctacac gctgttcgga
     1381 ggcaagaccg agctgctcgc cgccgtcatc gagcatgcgt tccgctcctt cggggacgcg
     1441 cagctcaccg cggagccgca gggcctccgc gcgctcggtg aggcgtatcg cgcctggtcg
     1501 ctggcccatc ctgccctctt ccggctgatg ttcggcgatc ggatgcccgc cgagcacgca
     1561 gcggtggagg ccgtggccgt gcagtcgctc gccccgctcg tgcgcacggt ctccggcatc
     1621 cggccaggac cccgcgagac cgcgcagcgc gacgccgtga tcatctggtc acaggtgcac
     1681 ggcgccgtcg ccctcgagct cgcgcaggcc gctcccgccg gggtcgactg ggacgcggtc
     1741 tacgccgggc tgctcgatgt cgtggagcgc tcgctcgcat agtcccgcca cgctccgaga
     1801 cccccggtca gcgtcgagac ccccagctgc gtgcgtctgc agctgggggt ctcggcaccg
     1861 agcaggggtc tcggcgaaac ctggtcaggc caggctgcgc tcggcggcct cgaccacgtt
     1921 cgtcatgaga agcgccaccg tcatcggacc gaccccgccg gggttcgggg agatgaagcc
     1981 ggcgacctcg gcgacgtccg ggtgcacgtc gccgtagacc ttcgacttgc cggtctcggc
     2041 atccgtctcc ctggtcacgc ccacgtcgag caccgcggcg ccgggcttca cgtcggatgc
     2101 cgtgatcagg tgcttcacac cggcgcccgc gacgatcacg tcagcctcgc gcaggtacgg
     2161 cgtcatgtcc ggagtgccgg tgtgcacctg cgtgactgtg gcgttgatca cgcgacgcgt
     2221 cagcagcagc cccatcggac ggccgatcgt gacacctcgg ccgacgacca ccacgtgctt
     2281 gccgttcagg tcgtagtcgt tgcgcagcag cagctcgatc acgccccgcg gggtgcacgg
     2341 cagcggcgtg tggatcgggc cgttgacgtt cagcaccagg cggccgaggt tggtcgggtg
     2401 cagtccgtcg gcatccttgg ccgggtcgat ccgctccagg atcgcgtcgg tgtcgaggtg
     2461 cttcggaagc ggcagctgca cgatgtagcc gtggcaggcg gggtcggcgt tgagctcgtc
     2521 gatcagcgcc tcgacctgct cctgcgtggc atcggcgggc agctcgcgct ggatcgagtt
     2581 catcccgatc gcctcggact gcttgtgctt catgccgacg tacagctggg acgccgggtc
     2641 ggcgcccacc aggaccgtgg cgatgcccgg gacgacgccg cgttcgcgca gcgcggccac
     2701 gcgttcggtg agctcgtcct tgatcgccgc cgcggcggcc ttgccgtcga ggaccttcgc
     2761 ggtcactgct gcaggcccgg gtacagcggg aaggccgtgg cgagagcgtc cacgcgcgtg
     2821 cgcagcgcct cgatgtcggc accgggctgc agcgcgaccg cgatgatgtc ggccacctcg
     2881 gtgaactccg cgtcgccgaa gccgcgggtc gcgagggccg gcgtgccgat gcgcagaccc
     2941 gaggtcacca tcggcggacg cgggtcgttc gggaccgcgt tgcggttcac ggtgatgtgg
     3001 atctcgtgca gcagatcctc tgcctgcttg ccgtcgatct cggcgtcgcg caggtcgacg
     3061 agcaccaggt gcacgtcggt tccgccggag cgcacggcga tgccggcatc cttcacgtcc
     3121 tgctgggtga ggcgctcggc gatgatcgcg gcaccgcgca gcacgcgctc ctgacgctcc
     3181 ttgaactcgg gggtcgcggc gagcttgaac gcggtcgcct tggcggcgat cacgtgcatg
     3241 agcgggccgc cctgctggcc ggggaacacg gccgagttga tcttcttggc gatgtcggcg
     3301 tcgttggtga ggatgaagcc cgagcgggga ccgccgatgg tcttgtgcac ggtcgaggag
     3361 accacgtggg cgtgcggcac cgggttcggg tgcagacctg ctgccacgag gccggcgaag
     3421 tgcgccatgt cgacccagag cagggcaccc acctcgtcgg cgatcgcgcg gaacgcggcg
     3481 aagtcgaggg tgcgcgggta ggcggaccag ccggcgatga tcaccttcgg cttgtgctcg
     3541 agggcgagcg cgcgcacctc gtccatgtcg atggtggagg tctcggggtt cacgccgtac
     3601 gcgacgatgt tgtacagccg gccggagaag ttgatcttca tgccgtgcgt gaggtggccg
     3661 ccctggtcga gggagagacc gagcagcgtg tcgccggggc gcgcgatggc gtgcagcacg
     3721 gcggcgttgg cggaggcgcc ggagtgcggc tggacgttcg cgaactcgga gccgaacagc
     3781 gccttggcgc gggcgatcgc cagctcctcg gcgacgtcga cctcttcgca gccgccgtag
     3841 tagcgacggc ccgggtagcc ctcggcgtac ttgttcgtca gcaccgagcc ctgcgactgc
     3901 agcacggaga cgggcacgaa gttctcggag gcgatcatct cgaggtacgt gcgctggcgg
     3961 ttcagctcgc gctcgagcac ctcggcgatc tcggggtcga cctcggcgag cggggcgtcg
     4021 aagtagcggt cggtcatggc ggtctcctgt tcacacgggg taaggggtgt gttcctcatc
     4081 ggcccaggcg cgcggtcgaa tcctcagagc cgctccccgg tggtgaccca cccagacgcc
     4141 agtcgcgacg caagaaagct tagcggatgg cgatgtccgc ggcccacggt cctcgcccgt
     4201 gtgacggtcg gcgcgctagg ttttgttcat ggtccttcct gatctcctcc cgctggtccc
     4261 ggcacccgtc tccgtcgtca tcggcgaggg ccggatgccg ctggcggatc ccctgcgcat
     4321 catcggcgac gacgccgcgg ccgatcagct cgccgccgcg atcgggcacc gcaccggcat
     4381 ccgcccggtc cgcgtggccg cggaggcgcg ccccggcgac gtcgtcgtgc ggatcgatcc
     4441 ggatgccgcg gccgaggcgt atgcgctgac gatcggcgag atcgtcgagc tgaccggctc
     4501 cgcggacggg ctgctgcacg gcatccgcac cctgatgcag ctgctggcgg acgaagggga
     4561 cggtcggtgg gcctggcgcc gcgcgacgat cgccgacgcc ccgcgcttcg cgtaccgcgg
     4621 cgtcatgctc gacgtcgccc gccacttctt cccggtcgac gaggtcgaga aggttatcga
     4681 ccggatcgcc gagctgaagt tcaaccacct gcacctgcac ctcagcgacg accagggctg
     4741 gcgcatccac atcgactcct ggccgctgct ctcgcagctc tcctcgacgg tgagcgccac
     4801 gggcgacggc cgggcgaccg gcggcggctg ctacagcaag gacgactacc gcgcgatcgt
     4861 ctcctacgcg gcgtcccgcg gcatcacgat cgtccccgag atcgacatgc ccggccacac
     4921 ccatgccatc ggaatcgcct atcccgagct cgtcgaggcg cccgtcatga acgagcacct
     4981 gctcgacgac gcgcagcgac tgggccaggc cctccccgag gcgggcgtgc cgtacaccgg
     5041 ctggggtgtc ggccactcca gcctgcgcat ccacgaggag cgcacgtacg agttcgtccg
     5101 ggacgtgctc gcggagctcg ccgagctcac gcccggcccg tacctgcaca tcggcggcga
     5161 cgagtccctg ggcacctccc ccgcggactt cgcctccttc atcgagcgcg cgaccgggat
     5221 cgtgcaggag ctcggcaaga cgcctgtcgc ctggcacgag gccggcgccg cagaggtcgc
     5281 acccggcacg gtgggccagt actggggcct gctcgcaccg gacccctcgc acgccgagca
     5341 caccgcgcac ttcgtggagc gcggcggacg cgtgatcctc tcgccgtcgg acgccgccta
     5401 cctcgacatg aagtacgacc acgacttccc gctcggcctc gcatgggcgg ggacggtgga
     5461 cctgcaccgc gcctacgact ggcagccgac cgcgcttctc gacatccccg cggatgccgt
     5521 cgccggcgtc gaggccccgt tgtggaccga gacggtggag agcctcgagg aggccgatca
     5581 gctgatgttc ccgcgcatcg cggccatcgc cgagatcgcc tggagcgcct ccgaccccac
     5641 tcgcgactgg aacgacttcc gcgagagact ggcagcgcag gcgccggcct ggcgcgccga
     5701 cggcatccga ttccaccccg tgcccgagat cgactggagc gaccgatgac cccgaccaca
     5761 gacttccgag gctcccgcgt gatccactcc gcgcggatcg tcgacggcga cggcagcacc
     5821 accgagaacg gctgggtgcg gttcgagggc ggcgtggtca tcgcacgcgg cacgggcgcg
     5881 gactggacgg acgccgacga cgtgatcgac gcgagcgcga tcggcggcgc ggatgccgtc
     5941 ctcaccccgg gcttcatcga catccacggg cacggcggag cgggc
//
LOCUS       DATMSX010000471         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_82810,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000471 DATMSX010000000
VERSION     DATMSX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82810"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VN041_15015"
     CDS             complement(<1..406)
                     /locus_tag="VN041_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30393.1"
                     /translation="MLTIRDLSVEITHGDRIVRPVTGVDLELHRGETLGIVGETGSGK
                     SMTGLAVMGMLPGGGRVVSGSIDFDGTELVGLGDAAYRRIRGNDISMVFQDSMTSLNP
                     TRSVGDQVAEPLQLHRGMSLRAARRSAAEMLAL"
     gene            complement(547..1587)
                     /locus_tag="VN041_15020"
     CDS             complement(547..1587)
                     /locus_tag="VN041_15020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWU30394.1"
                     /translation="MMNDLHRRAVVADAHNDLLCAVASRPPEEWSSFFRTRWLPQLQA
                     GGVDLQVLPVFIDDPYRPEGALRRTLRMIEAAHRLAEGNADAVALCLTGEDVDGAIAG
                     GRIALVLALEGMPGIGEDVELLQTVHRLGVRIGSIAHFGRAAFGDGSGEDAADSRLTR
                     AGVRAVAEMERLGMLFDLSHLGAGGVDHVLELATRPVMATHSSARALRDHHRNLSDEQ
                     IRGIAATGGVVCVNFFAPFLHESEYTLDVLVDHLEHVASVAGVDRVGLGPDFVREVLD
                     DTTPPCCVVDTVEGVPADVYLPGLEGPEGLPLVTDALLRRGWAESEVLAVLGGNLQRL
                     LRAELGVPGPGV"
     gene            complement(1584..2963)
                     /locus_tag="VN041_15025"
     CDS             complement(1584..2963)
                     /locus_tag="VN041_15025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWU30395.1"
                     /translation="MPELARIREWVEERMPGLLAEHRVPALSLAVAAGDEICEYATGL
                     LSTTTGVTADTDSVFQIGSVTKVLTATLVMQLVEEGILDLDAPVREVLPGFRVADAAA
                     SEATTVRHLLTHTSGFEGDVFVDTGAGDDCLERYVEVLAGTPQLFAPGTMFSYNNAGF
                     STLGRIIEVLRGEPYERVLRRRLLDPLGLRHASIDAGEAILHRAAVGHLVSAEGALEP
                     TAVWSMERSGAPAGSRLAMTARDLLAFGRMHLAGGAAGDRARLLSEDSVSLMRAEQVR
                     LPDIHQGSGWGLGWELFERSGTTVVGHDGSTIGQSARLRLLPSEGVVIAILANGGETH
                     AVFAEIIDRMLHDLAGLAPEPPLVPTGPAPRDATRFLGRYESSTAITTVSRTDDGRVW
                     LDREPCGVVAELGDQPFRTELLGWHEGVLLPVEAEGGIRQPVAFLGDDGTGRASHLHT
                     GRADRRREA"
     gene            complement(3066..3569)
                     /locus_tag="VN041_15030"
     CDS             complement(3066..3569)
                     /locus_tag="VN041_15030"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWU30396.1"
                     /translation="MNALIQTIDTPDGALTILADETQRVLASGWSDDHAAIIARVHAS
                     LRPDSIAEGRTDAADAAQAYYAGDLDAITTVAVRQHGTEMQRVGWEALRRITPGQPLS
                     YTEFAIELGSPRAVRAAASVCARNAPALFVPCHRVLRSDGSLGGFAWGLDVKRSLLER
                     EAAGSAR"
BASE COUNT          531 a         1308 c         1344 g          530 t
ORIGIN      
        1 ccagcgcgag catctcggcg gcgctgcggc gggccgcccg cagcgacatg cctcggtgca
       61 gctgcagcgg ctcggcgacc tgatcgccga cgctgcgggt cgggttgagc gaggtcatcg
      121 agtcctggaa gaccatggag atgtcgttgc cgcggatgcg ccggtaggcg gcgtcgccca
      181 ggcccacgag ctccgtgccg tcgaaatcga tggatccgct caccacgcgg ccgccgccgg
      241 gcagcatgcc catgaccgcg agtccggtca tcgatttgcc ggagccggtc tcgccgacga
      301 tgccgagggt ctcgccgcga tgcagctcga ggtctacgcc ggtcaccggg cgcacgatgc
      361 ggtcgccgtg cgtgatctcg accgacaggt cccgaatcgt cagcatgcgc gcgctccctt
      421 gcgactccct gcagctcccg gacgtgcgga caggtgctgc gactcttcga tactgcggca
      481 acgggcagtc gacgcgttcg tccgagcctg cgaacgacgt cgcgtccgtt cgtcgacctc
      541 gacgaatcag acgccggggc ccgggacgcc gagctcggcg cgcagcagcc gctgcagatt
      601 gccgccgagc accgccagga cttcggactc ggcccagccg cggcgcagca gggcatccgt
      661 gacgagcggc agcccctcgg ggccctcgag cccggggagg tagacgtcgg caggcacgcc
      721 ctcgacggtg tccaccacgc agcagggcgg ggtggtgtcg tcgagcactt cgcgcacgaa
      781 gtccggcccg agtcccaccc gatcgacgcc ggccaccgag gcgacgtgtt cgaggtgatc
      841 gacgagcacg tcgagcgtgt actcgctctc gtgcaggaag ggcgcgaaga agttcacgca
      901 gacgaccccg ccggtggcgg cgattccgcg gatctgctcg tcgctgaggt tgcggtgatg
      961 gtcgcgcagc gcccgtgcgg acgagtgggt ggccatgacc ggccgtgtgg cgagctcgag
     1021 cacgtggtcg acgccgccgg cacccaggtg actgaggtcg aacagcatcc cgagccgctc
     1081 catctccgcc acggcccgca cccccgcgcg ggtgagcctg ctgtccgccg cgtcctcccc
     1141 gctgccgtca ccgaacgccg cccgtccgaa gtgcgcgatc gatccgatgc gcacgccgag
     1201 acggtgcacg gtctgcagca gctcgacgtc ctccccgatg cccggcatcc cctccagcgc
     1261 gaggacgagt gcgatgcgtc cgccggcgat ggcaccatcg acgtcctcgc cggtgagaca
     1321 gagcgcgaca gcatccgcgt tcccctcggc caggcgatgg gcggcctcga tcatgcgcag
     1381 cgtgcgccgc agcgcgcctt cgggccggta cgggtcgtcg atgaacacgg ggagcacctg
     1441 cagatcgacg ccgccggcct gcagctgcgg caaccagcgg gtccggaaga acgacgacca
     1501 ctcctccggc gggcggctcg cgacggcgca gagcaggtcg ttgtgcgcgt cggcgacgac
     1561 ggcccggcga tgcaagtcgt tcatcatgct tcccttcttc tgtccgcacg tccggtgtgc
     1621 aggtgcgatg cccgtccagt gccgtcgtcg cccaggaagg cgaccggctg gcggatgccg
     1681 ccctcggcct cgacggggag gagcacgccc tcgtgccagc cgagcagttc ggtgcggaag
     1741 ggctgatcgc cgagctccgc cacgacaccg catggttcgc ggtccagcca cacccggccg
     1801 tcatcggtcc ggctgacggt ggtgatcgcc gtgctcgact cgtagcgacc caggaagcgc
     1861 gtcgcatccc gcggggcggg gccggtgggt acgagcggcg gctcgggggc caggcccgcc
     1921 aggtcgtgca gcatccggtc gatgatctcc gcgaacacgg catgcgtctc accgccgttg
     1981 gcgaggatcg cgatcacgac tccctccgag ggcagcaggc gcagtcgtgc cgactggccg
     2041 atggtgctgc cgtcgtgccc gacgaccgtc gtcccggatc tttcgaacag ctcccagccg
     2101 agtccccagc ctgacccctg gtggatgtcg ggcaggcgca cctgctcggc acgcatgagc
     2161 gagacggagt cctccgagag caggcgcgcc ctatcgccgg cggcaccgcc cgcgaggtgc
     2221 atgcgtccga acgcgagcag atcgcgcgcc gtcatcgcga gcctcgatcc tgccggggca
     2281 cccgagcgct ccatcgacca caccgctgtc ggctcgagcg cgccctcggc ggagacgagg
     2341 tggccgacgg cggcacggtg caggatcgcc tcgcccgcat cgatcgatgc gtgccgcagg
     2401 ccgagcggat cgagcagcct ccgacgcagc acacgctcgt atggctcgcc gcggagcacc
     2461 tcgatgatgc gcccgagcgt cgagaacccc gcgttgttgt acgagaacat ggttcccggc
     2521 gcgaacagct ggggcgtgcc ggcgagcacc tcgacgtacc gctccaggca gtcgtcgccg
     2581 gcgccggtgt cgacgaagac gtcgccctcg aacccggagg tgtgggtcag caggtggcgg
     2641 accgtggtcg cctcggatgc cgcggcatcc gccacccgga atcccggcag cacctcgcgc
     2701 accggggcat cgaggtcgag gatcccctcc tcgaccagct gcatgacgag cgttgcggtc
     2761 agcaccttcg tgacggagcc gatctggaag acggagtccg tgtcggcggt cacaccggtc
     2821 gtggtgctga gcagtccggt ggcgtactcg cagatctcgt ctccggcggc gacggccagc
     2881 gacagcgcgg gcacgcggtg ctcggcgagc agccccggca tccgctcctc gacccattcg
     2941 cggatccgtg ccagctccgg catgcgccct cctcgatgac tgagacgtct cgagcctagg
     3001 ggcgtcgtcc tgccggagtt tcgtccggca ggacgactgc gggcggtccg agttgtcgag
     3061 atcgctcagc gagcggagcc ggcggcctcc ctctcgagca ggctgcgctt gacatcgagg
     3121 ccccaggcga agcctccgag gctcccgtcc gagcgcagca cgcgatggca gggcacgaac
     3181 agggcgggcg cgttgcgggc acagacggag gcggcggcgc gcaccgcccg cgggctgccg
     3241 agctcgatgg cgaactcggt gtaggacagg ggctgtcccg gagtgatgcg tcgcagcgcc
     3301 tcccagccga cgcgctgcat ctcggtgccg tgctggcgca cggcgacggt ggtgatggcg
     3361 tcgaggtcgc cggcgtagta cgcctgcgcc gcgtcggcgg catccgtcct cccctccgcg
     3421 atgctgtcag ggcgcagcga ggcgtgcacg cgcgcgatga tcgcggcgtg gtcgtcgctc
     3481 cagccggagg cgagcacgcg ctgcgtctca tcggcgagaa tcgtgagggc gccgtccggg
     3541 gtgtcgatcg tctggatgag ggcgttcatg agagctcctt gctcgaggtc ttcggctttg
     3601 cggttcgcgc gacgcggacg ggtgcggcgc gccagaggtg ggcggtgaga tagctgcgcc
     3661 agggggcgat gcgctcggcc caggccgtga gggccgccgg ttcggcgggc agg
//
LOCUS       DATMSX010000472         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_16601,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000472 DATMSX010000000
VERSION     DATMSX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16601"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VN041_15035"
     CDS             <1..669
                     /locus_tag="VN041_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30397.1"
                     /translation="GAPSPSASVAAAARPDPAGSCTSIDLDAETATGAQLGACFSDAL
                     HVLGTFRAKVDTGDELQQAEVRLRPDVAIHGKTTGADPDEVVFVDDTAYKNDGGGWIK
                     GDPGSDDPEKMVIGNAGRLLVAVFAGDVLRQSVEACPVWNLLPAKEKMTLPDDMVVEA
                     RVFECAAPYVMIGSTITDSRLLFEQDWTPVSATYTATGFGQTVRAEQWWYDFGADLEI
                     TAPI"
     gene            694..912
                     /locus_tag="VN041_15040"
     CDS             694..912
                     /locus_tag="VN041_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30398.1"
                     /translation="MGLFSQKQEDPGAWAALPGEPLEEKANERLDEAPTMDLMDVGLG
                     ALYTSTVIPVAPPAPEASDITESGEPED"
     gene            complement(920..1423)
                     /locus_tag="VN041_15045"
     CDS             complement(920..1423)
                     /locus_tag="VN041_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30399.1"
                     /translation="MSGFAAKVVIILIVVTLVVVLVARFFRRVTPAKPGTRRQRMPLL
                     VPLGGAVLIVAGSLLALASFSSRYTPELLPARIASAVVILAGLAALLAYRNWYLEVFS
                     DAVRFRTLLGREKRIDYRDIASFRLKKAAGRERLTVRSRDGVRLSVDTGRYSVAPLLA
                     AARSAAA"
     gene            1479..>1991
                     /locus_tag="VN041_15050"
     CDS             1479..>1991
                     /locus_tag="VN041_15050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HWU30400.1"
                     /translation="MTIIAAADGSALGNPGPNGWAWYIDDANWAAGGSPHGTNNQGEL
                     QAVLELLRATVGTDEKLLIHCDSRYVIDSVTKWMPGWKRRGWRKSDGGPVLNRDLLEG
                     IDEALRGRDVEFDWVKGHAGHPLNEAADERANAAAKAYQQKQEPKRGPGFSSASDGGA
                     ALAGAMALEAD"
BASE COUNT          350 a          659 c          735 g          247 t
ORIGIN      
        1 ggagcgccgt cgccctcggc atcggtcgcc gcggccgcac gacccgaccc cgccggcagc
       61 tgcacgagca tcgacctgga cgccgagacc gcgacgggag cgcagctcgg cgcgtgcttc
      121 agtgacgcgc tgcacgtgct cggcaccttc cgggcgaagg tcgacacggg agacgagctg
      181 cagcaggccg aggtgcggct gcgccccgac gtcgcgatcc acggcaagac cacgggagcc
      241 gatcccgatg aggtcgtctt cgtcgacgac accgcgtaca agaacgacgg cggcggctgg
      301 atcaagggcg atcccggctc cgacgacccc gagaagatgg tcatcggcaa tgccggccgg
      361 ctgctcgtcg cggtgttcgc cggcgatgtg ctacgccagt cggtggaggc atgcccggtg
      421 tggaacctgc tgccggcgaa ggagaagatg accctcccgg acgacatggt cgtggaggcc
      481 cgggtgttcg agtgcgccgc tccctacgtg atgatcggct cgaccatcac cgacagccgg
      541 ctgctcttcg agcaggactg gacgcccgtg agcgcgacct acacggcgac cggattcggc
      601 cagaccgtac gcgccgagca gtggtggtac gacttcggcg ccgatctgga gatcaccgcc
      661 ccgatctgag cgggccgggg cgagaatgga cgcatgggac tgttctcgca gaagcaggag
      721 gatccgggcg cgtgggcggc attgcccggg gaaccgctcg aggagaaggc gaacgagcgc
      781 ctcgatgagg ccccgaccat ggacctgatg gacgtcgggc tcggggcgct ctacaccagc
      841 acggtcattc cggtcgcacc acccgccccc gaggcatccg acatcacgga gtccggcgag
      901 cccgaggact gagccgcgct caggctgccg cgctgcgcgc ggcggcgagg agcggcgcca
      961 cgctgtaccg gcctgtgtcg acggacaacc gcacgccgtc gcgggatcgc acggtgagac
     1021 gctcccggcc ggcggccttc ttcagccgga acgaggcgat gtcgcggtag tcgatgcgct
     1081 tctcgcgccc gagaagggtg cggaatcgca cggcatccga gaacacctcg aggtaccagt
     1141 tgcggtaggc gagcagcgct gccagcccgg cgaggatcac gaccgccgag gcgatgcggg
     1201 cggggagcag ctcgggcgtg taccgcgacg agaacgaggc gagcgcgagc aatgaaccgg
     1261 ccacgatcag cacggctccc ccgagcggga cgagcagcgg catccgctgc ctcctcgtcc
     1321 ccggcttcgc gggcgtcacg cgccggaaga agcgggcgac gaggacgacg accagcgtga
     1381 ccacgatgag gatgatcacg actttggcgg cgaacccgga catccctcga cgataccgac
     1441 tgtcgggccg tgtcggatcg acgccctacg gttgaaggat gacgatcatc gccgccgcag
     1501 acggctccgc gctgggcaac ccgggcccga acggatgggc ctggtacatc gacgacgcga
     1561 actgggctgc cggtggatcg cctcacggca ccaacaacca gggtgagctg caggcggtgc
     1621 tcgagctgct gcgggcgacg gtgggcaccg atgagaagct gctcatccac tgcgacagcc
     1681 ggtacgtgat cgactccgtc accaagtgga tgccgggatg gaagcgccgc ggctggcgca
     1741 agtccgacgg cggtccggtg ctcaaccgcg acctgctgga gggcatcgac gaagcgctgc
     1801 gcggacggga cgtggagttc gactgggtga aggggcacgc cgggcatccg ctcaacgagg
     1861 ccgcggacga gcgcgccaac gccgcggcga aggcgtacca gcagaagcag gagccgaagc
     1921 gcgggcccgg gttctcgtcg gcatccgacg gtggcgcggc gctcgcggga gcgatggcgc
     1981 tcgaggcgga t
//
LOCUS       DATMSX010000473        10116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_7879,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000473 DATMSX010000000
VERSION     DATMSX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7879"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VN041_15055"
     CDS             complement(<1..1290)
                     /locus_tag="VN041_15055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30401.1"
                     /translation="MGVGSALLPESASELPDAVYLVLSSRLIPGMDGGYTIATLARAR
                     QLAGAGVGDGAGPQLLTFDPGTAADHAEHRRVFAEQGMLLDPARMRNLFDEAVAADGG
                     AAGWLLDAADPAVTADPDVEYRELTDAEGRRFAALPVIPANPDWHLTEEPVLVYSAAG
                     DVAGALHGFRGLYRAWLEHVTASFGDHRIIVICESRQLGELVADWSDPRVRLIHTIHT
                     MHVEAPYTPDASMNTLWTRWFRVADRFDAVVWPTTTQRDDVVARFGASANHVVVPNPI
                     APVESRQADREGGLVVVLGRLASGKRVDHAIRAFLAADVPGARMEIWGGGPEQDRLQT
                     LIDELGAGERVVLAGYTDAPGTVLDRASLLVTSTAFEGQPLGIVEALLHGTPVVSYDV
                     RYGIRDVLGHGGGVLVPAGDVDALSLAIRSLLTDPERL"
     gene            complement(1281..2867)
                     /gene="guaA"
                     /locus_tag="VN041_15060"
     CDS             complement(1281..2867)
                     /gene="guaA"
                     /locus_tag="VN041_15060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWU30402.1"
                     /translation="MTEQSETQQRPALVVDFGAQYAQLIARRVREAGVYSEIVPHTAT
                     AQEIAAKNPVAIILSGGPSSVYEEGAPKLDPAVFDLGVPTLGICYGFQYMAQTLGGEV
                     AHTGLREYGATDAVISGDGGTLLGGQPAEQNVWMSHGDQVAKAPEGFEVLATTEATKV
                     AAFANEERRFYGVQWHPEVKHSDHGQRVLENFLHKGAGLASDWNPDNVIAEQIERIRE
                     QVGDARVISALSGGVDSAVSTALVHKAIGDQLTAVFVDHGLLRKGEREQVEKDYVEST
                     GVRLITVDAAETFLGHLKGVTDPEEKRKIIGREFIRAFEKVQLDLVEEAKAAGGAPVK
                     FLVQGTLYPDVVESGGGAGTANIKSHHNVGGLPEDLDFELIEPLRALFKDEVRAIGRE
                     LGIPEAIVGRQPFPGPGLGIRIIGEVTADRLEILREADAIAREELTKAGLDQEIWQCP
                     VVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRLPYDVLSKISNRITNGVR
                     DVNRVVLDVTSKPPGTIEWE"
     gene            complement(2864..3376)
                     /locus_tag="VN041_15065"
     CDS             complement(2864..3376)
                     /locus_tag="VN041_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HWU30403.1"
                     /translation="MPEPKVASFPAIRGALKFYQIASIITGVMLLLLLAEMVLKYTPI
                     HLELFAGGSGGPLWFAGVIAGPDCQWWSLFAPWTNSCEMTSLGDGFNISLFILVAHGW
                     FYVVYLFACFRMWSLMRWPFPRFILLALGGVIPLLSFFMEAIVAREVKSYLATREAEE
                     ASVTAPEGVR"
     gene            3480..4103
                     /locus_tag="VN041_15070"
     CDS             3480..4103
                     /locus_tag="VN041_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HWU30404.1"
                     /translation="MNTTIDSVTLDVPDADAARSFYAQAFDLGDRLHVREADADSSGF
                     RGYTLSLVVSQPANVHALVDAAVAAGATVIKPVSKSLWGVGGTVQAPDGAIWKVATSA
                     KKDTAAPSRAVDEVILLLGAADVVASKTFYAERGIPIGKSFGRSYVQFDTGTSPIGLG
                     LYRHASLAKDAGVPADGSGSHRLTINGVLGAAVDPDGFVWAPVTVTA"
     gene            complement(4184..8761)
                     /locus_tag="VN041_15075"
     CDS             complement(4184..8761)
                     /locus_tag="VN041_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HWU30405.1"
                     /translation="MTTVFALGFGSLAASPAFASTDGTGVVINEAYLSGGSAGAAFKN
                     KFVELYNPTAAAVSLDGMSLQYRSATGTGSFGGVVALSGTIPAHGYFLVQGGTNSGTS
                     TAPALPTADVTGTALNPSGTTGTVALVKGTAPLTLAAGNAAGAEGVIDLLGYGSSNTF
                     EGAVATAPSSNTDVKSLNRAGGKDTDSNKDDFSLSASVSPTNSKGETGATDPGTDPGP
                     QPGEKVSIADVQGTGDTSPLVGKTVTVEGVITADYRVGGYNGVVIQTQGTGGETKTAG
                     KSDGVFVYLNGKTVPGKIGDLVSVNGAVSEYYGQTQITPASASDVSVVTAGVGVPAPV
                     ALPTSVVGTDREQYENMLVAPAGDYLLASSHQLYNYGTLWLNPGELNVKSTELKRPGA
                     EADAIAAANRADRILLDDGYSVGIGKAGHTGDQPYFTKDVVVRNGDAVDFGGKGFVLQ
                     YDHDDWRLQPVTPLNSTSPASEKVTFKTNNPRPSVPSVGGDVQIASFNVYNYFTTLSS
                     ENPDARGAKDAAQFAIQKSKIVAAINGTGAEIVSLMEIENGPKLGEPLDTALADLVSG
                     LNAAAGSEVWAYVPTPKALDDAAITDYITSAIIYKKAAVTPKGAAQTIIDETVWGNAR
                     EPIAQAFDIDGRTVTVVANHLKSKSGTGTQPADGQGFFNADRVAQAKSLLAFTEKLKT
                     DSGSDDMLLIGDFNAYGHEDPIDVFTSQGWLDTVPSKAKGQYTYSFDGELGSLDHVIA
                     SPSLAASVTGAGVWGINSPEWSDRGYAFGATEAGTPFRSSDHDPILVGVSATAPPVSI
                     DIATINDFHGRIEADGAAAGAAVLAGAVRDVRAKNPNTIFAAAGDLVGASTFTSFIQD
                     DNPTIDALNAAGLEVSAAGNHEFDKGWADLRDRIVPRADWEYISSNVFMKGADKTALA
                     PSWVKDVDGVRVGFVGAVTEELDNLVSPDGIADLDVRDIVASVNTAAAALRDGDPSNG
                     EADVIILLVHEGATTTSVDSITPDSALGKIVYGVGKDVNAIVSAHTHLAYNHVIDGRP
                     VVSAGQYGENMGVMNLQVDPKTHKLLSISNEIKPLAHQNPDKTWTANYPAVPEVQTIV
                     DTAKKEADVLGNVKVGEITADFNRALQSDGKTENRGGESTIGNFIADVQQSSTGAELA
                     LMNPGGIRTVMAYKGTSASDPDGNLTYREAASVQPFANTLVTLSLTGEQLKAVLEEQW
                     QPAGSSRPFLKLGVSKGLTYTYDPNAAQGSHITSITLNGTPVDPAKSYLVAANSFLAA
                     GGDNFFTLAKGTGKKDTGKVDLQSMVDWFTANKTATPDLGQRAVGAVVSPAAGTDGYK
                     AGDQVTVDLSSLAFSAREQVPTEVAFSIGDTQLGTAPVTVAVKDAYDEAGAATFTFSV
                     PADAAGKTLTATPNVGTAVVLPVTFAADEEPGEFAGTISLGSSKVAAGQALTITGEGY
                     QPGETVAVRLQPKKGDAIDLGTVKVGEDGSFSASVTVPKATKPGPYTVSVSQADGDAA
                     TAVVHVNRKGGLGNVVQGIVDWVLGLISGWF"
     gene            complement(9042..9884)
                     /locus_tag="VN041_15080"
     CDS             complement(9042..9884)
                     /locus_tag="VN041_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HWU30406.1"
                     /translation="MTNSAPLAPQEFPPTLREVMLRRRWIGVLALCLVVAGVFAWLGQ
                     WQLERAIDTTPPAPGATEKVRPLTDVIEPGKYLDQSLGGQRVRTSGTWVAGDFIVISQ
                     RINDGTEGYWVTGQLRVADRTSIAVAVGWAATRKQADAAVAALEKQGAVTAEITGRLI
                     DDEGVEVPPRSDPMELNRMSPAALLSRWHDIDGLDVYRPYLASATASAGLVDISSPPP
                     QEQSPINWLNIFYAAEWAVFAGFAFYLWYRLAKDAWEREVEEFEDAANAGEPAAAPED
                     EQAS"
     gene            complement(9881..>10116)
                     /locus_tag="VN041_15085"
     CDS             complement(9881..>10116)
                     /locus_tag="VN041_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWU30407.1"
                     /translation="QGFHWGAEAHHPQLPRGRRVAVDGVANLEEILYGPAPVADGTAN
                     LIGALRKSMATTGYSDLKEFQRVEVVLAPYELG"
BASE COUNT         1485 a         3414 c         3560 g         1657 t
ORIGIN      
        1 aagacgctcg gggtcggtca gcagcgagcg gatggcgagg ctcaacgcgt cgacgtcccc
       61 cgcggggacg agcaccccgc cgccgtggcc caacacgtcg cggatgccgt aacgcacgtc
      121 atacgacacg acaggcgtgc cgtgcagcag cgcctcgacg atccccagcg gctgcccttc
      181 gaacgcggtc gacgtgacca gcagggaagc ccgatccaga actgtccccg gtgcatcggt
      241 gtagcccgcg agcacgaccc gttctcccgc accgagctcg tcgatcagag tctgcaggcg
      301 gtcctgctcg ggccctccgc cccagatctc catgcgagcg cctggtacgt ccgcggcgag
      361 gaaggcacgg atggcatggt cgacgcgttt ccccgaggcc aggcgaccga gcaccaccac
      421 gagcccgccc tcgcggtcgg cctgacgtga ctcgaccggg gcgatcggat tcggcacgac
      481 cacatgattc gccgaagccc cgaaccgcgc gaccacatcg tcacgctggg tggtggtcgg
      541 ccagaccacg gcgtcgaagc ggtcggccac gcggaaccag cgcgtccaca gcgtgttcat
      601 gctcgcgtcc ggcgtgtagg gcgcctcgac gtgcatggtg tggatcgtgt ggatgagacg
      661 cacgcgcgga tcggaccagt ccgccaccag ctcgccgagc tgccgggact cgcagatcac
      721 gatgatgcgg tgatcaccga aggaggcggt cacgtgctcg agccacgccc ggtacagccc
      781 gcggaagccg tgcagggcgc cggcgacatc gccggccgcg ctgtacacga gcaccggctc
      841 ctcggtgagg tgccagtccg ggttcgcggg gatgaccggg agggccgcga accggcgtcc
      901 ctccgcatcg gtcagctccc ggtactcgac atcggggtcg gccgtcacgg cggggtcggc
      961 ggcatcgagc agccatcccg ctgctcctcc gtcggctgcg accgcctcgt cgaagaggtt
     1021 gcgcatgcgg gcggggtcga gcagcatccc ctgttcggcg aacacgcggc gatgctccgc
     1081 atggtcggcg gccgttcccg gatcgaacgt cagcagctgc ggtcccgcgc cgtcgcccac
     1141 tccggctccg gccagctgcc gggcacgggc gagcgtcgcg atcgtgtacc cgccatccat
     1201 cccgggaatg agccggctgg acagcaccag atacacggcg tcgggaagct ccgacgccga
     1261 ctcggggagc agggcgctcc ctactcccac tcgatcgtcc ccggcggctt cgaggtgacg
     1321 tcgaggacga cgcggttcac gtcgcggacg ccgttggtga tgcggttcga gatcttcgac
     1381 agcacgtcgt acggcagtcg cgtccagtcg gcggtcatgg cgtcctcgct ggacaccggt
     1441 cgcagcacga tcgggtgacc gtacgtgcgg ccgtcgccct gcacgcccac cgaacgcacg
     1501 tcggcgagga gcacgacggg gcactgccag atctcctggt cgagcccggc cttggtcagc
     1561 tcctcgcggg cgatggcgtc ggcctcacgc agaatctcga gacggtcagc ggtgacctca
     1621 ccgatgatcc ggataccgag gccgggcccc ggaaacggct ggcggccgac gatcgcctcg
     1681 gggatgccga gctcacggcc gatcgcccgc acctcgtcct tgaacagggc gcgcagcggc
     1741 tcgatgagct cgaaatcgag gtcttcggga agcccgccca cgttgtggtg ggacttgatg
     1801 ttcgcggtcc ccgctccccc tccggactcg accacatcgg ggtagagcgt gccctgcacg
     1861 aggaacttca ccggagcgcc gcctgcggcc ttcgcctcct cgacgaggtc gagctgcacc
     1921 ttctcgaacg cgcggatgaa ctcgcggccg atgatcttgc gcttctcctc ggggtcggtc
     1981 acacccttca ggtggccgag gaacgtctct gccgcgtcga cagtgatgag acgcacgccg
     2041 gtggactcga cgtagtcctt ctccacctgc tcgcgttcgc ccttgcggag cagaccgtgg
     2101 tcgacgaaca cggccgtgag ctggtcgccg atggccttgt gcacgagggc cgtggagacc
     2161 gcggagtcca caccgccgga gagcgcggag atgacgcgtg catcgccgac ctgttcgcgg
     2221 atgcgctcga tctgctcggc gatcacgttg tcggggttcc agtcggaggc gagccccgcg
     2281 cccttgtgga ggaagttctc cagcacgcgc tgaccgtgat cggagtgctt gacctcgggg
     2341 tgccactgca caccgtagaa ccggcgctcc tcgttcgcga acgccgcgac cttcgtggcc
     2401 tccgtcgtcg cgaggacctc gaagccctcc ggcgccttgg cgacctggtc gccgtggctc
     2461 atccacacgt tctgctccgc gggctggccg cccagcagcg tgccgccgtc gcccgagatc
     2521 acggcatccg tcgcaccgta ctcgcgcagg ccggtgtggg cgacctctcc gccgagggtc
     2581 tgcgccatgt actggaagcc gtagcagatg ccgagcgtgg gcacgccgag gtcgaagacc
     2641 gccgggtcga gcttcggggc gccctcctcg tacaccgacg atggaccgcc ggagaggatg
     2701 atcgcgacgg ggttcttggc ggcgatctcc tgggccgtgg ccgtgtgcgg cacgatctcg
     2761 ctgtagacgc ccgcttcacg cacgcgacgg gcaatcaact gcgcatactg tgcgccgaaa
     2821 tcgacgacga gcgcggggcg ctgctgggtc tcggactgtt cggtcaacgg acaccttccg
     2881 gggcggtgac ggaggcctcc tcggcctccc gggtggcgag ataggacttc acttcacggg
     2941 cgacgatcgc ctccatgaag aacgacagga gcgggatcac accgccgagc gcgagcagga
     3001 tgaagcgcgg gaacggccag cgcatcaggc tccacatgcg gaagcacgcg aagaggtaga
     3061 cgacgtagaa ccagccgtgt gccaccagga tgaacagtga gatgttgaac ccgtccccga
     3121 gggaggtcat ctcgcacgag ttcgtccacg gcgcgaacag cgaccaccac tggcagtccg
     3181 ggcccgcgat cacgccagcg aaccacagcg gtccgccgga gcctcccgcg aagagctcga
     3241 ggtggatcgg cgtgtacttc agcaccatct cggcgagcag caggagcagc atgactcccg
     3301 tgatgatcga cgcgatctgg tagaacttca gcgcaccgcg gatcgccgga aagctggcga
     3361 ctttcggttc gggcatgcct ccagtctagt cggcgggaaa tactgccgaa tccggcacct
     3421 tccaccatcg gtcaggaatc gagaagcatc gcagatccgt cgtccgtagc gtcgacgaca
     3481 tgaacaccac gatcgattcc gtcaccctcg acgtcccgga tgcggacgcc gcacgctcct
     3541 tctacgcgca ggcgttcgac ctcggagacc gactgcacgt gcgcgaagcg gacgccgaca
     3601 gctccggctt ccgcggatac accctgtcgc tggtcgtctc gcagccggcg aacgtccatg
     3661 ccctggtcga tgccgccgtc gccgcgggcg ccaccgtcat caagccggtg tcgaagtcgc
     3721 tctggggcgt cggcggtacg gttcaggcac cggacggcgc gatctggaag gtcgccacct
     3781 cggcgaaaaa ggacaccgca gcgccgagcc gcgctgtgga cgaggtcatc cttcttctcg
     3841 gcgccgctga cgtcgtcgcg tcgaagacgt tctacgccga acggggcatc cccatcggga
     3901 agagcttcgg ccgcagctac gtgcagttcg acaccgggac gagcccgatc gggctcggcc
     3961 tctatcggca cgcgtccttg gccaaggacg ccggcgtccc cgccgacgga tccggctcgc
     4021 accgtctcac catcaacggc gtgctgggcg ccgccgtcga ccccgacggc ttcgtctggg
     4081 ctccggtcac cgtcaccgcc tgacggacca acggacgacg gaggccgccg ggatcgctcc
     4141 cgacggcctc tccgtctgcg ctgtcagctg gcgccgagcc cgatcagaac catccggaga
     4201 tcaggccgag gacccagtcg acgatgccct gcacgacgtt cccgaggccg cccttgcggt
     4261 tgacgtggac caccgcggtc gcggcgtcgc cgtcggcctg cgagaccgag accgtgtacg
     4321 gaccaggctt ggtcgccttg ggcacggtga cggatgccga gaacgagccg tcctcgccga
     4381 ccttcacggt gccgaggtcg atcgcgtcgc ccttcttcgg ctgcagccgg acggccaccg
     4441 tctcgccggg ctggtagccc tcaccggtga tcgtcagcgc ctgaccggcg gccaccttgc
     4501 tcgatccgag cgagatcgtg ccggcgaact caccgggctc ctcgtcagcg gcgaaggtca
     4561 ccggcagcac cacggccgtg ccgacgttcg gcgtcgccgt gagcgtcttg cccgcggcat
     4621 ccgccggaac gctgaaggtg aacgtcgcgg caccggcctc gtcgtaggcg tccttcacag
     4681 cgaccgtgac gggggcggtt cccagctgcg tgtcgccgat cgagaacgcc acctcggtcg
     4741 gcacctgctc gcgtgcgctg aacgccagcg aggagaggtc gaccgtcacc tggtcgccgg
     4801 ccttgtagcc gtcggtgccg gccgccgggg agacgaccgc gcccacggcg cgctgaccga
     4861 ggtcgggggt cgccgtcttg ttggccgtga accagtcgac catcgactgc aggtcgacct
     4921 tgccggtgtc cttcttaccg gtgcccttgg cgagcgtgaa gaagttgtct ccacccgccg
     4981 cgaggaacga gttcgccgcg accaggtacg acttcgccgg gtcgacgggg gtgccgttca
     5041 gggtgatcga ggtgatgtgc gagccctgag ccgcgttcgg gtcgtacgtg taggtcagac
     5101 ccttcgagac gccgagcttg aggaacgggc gcgacgagcc cgcgggctgc cactgctcct
     5161 cgagcacggc cttcagctgc tcacccgtga ggctgagcgt gaccagcgtg ttggcgaacg
     5221 gctgcaccga tgccgcctca cggtaggtca ggttgccgtc ggggtcgctc gcagaggtgc
     5281 ccttgtacgc catgaccgtg cggatgccgc ccgggttcat cagcgcgagc tccgccccgg
     5341 tggacgactg ctgcacgtcg gcgatgaagt tgccgatggt ggactcgcca ccgcggttct
     5401 ccgtcttgcc gtcgctctgc aatgcccggt tgaagtcggc cgtgatctcg cccaccttga
     5461 cgttgccgag cacgtcggct tccttcttgg cggtgtcgac gatcgtctgg acctcgggga
     5521 cggccgggta gttcgccgtc cacgtcttgt cggggttctg atgcgccagc ggcttgatct
     5581 cgttggagat cgagagcagc ttgtgcgtct tcgggtcgac ctgcaggttc atcacgccca
     5641 tgttctcgcc gtactggccc gccgagacga ccgggcggcc gtcgatgacg tggttgtagg
     5701 cgaggtgggt gtgcgccgag acgatcgcgt tgacatcctt gccgacgccg tagacgatct
     5761 tgccgagcgc cgagtcgggc gtgatgctgt cgaccgacgt ggtggtggct ccctcgtgga
     5821 ccaggaggat gatgacatcc gcctcgccgt tgctcgggtc tccgtcgcgc agagccgcgg
     5881 cggcggtgtt gaccgaggcg acgatgtcgc gcacgtcgag gtcggcgatg ccgtccggcg
     5941 agacgaggtt gtcgagctcc tcggtgaccg cgcccacgaa gcccacacgg acgccgtcga
     6001 cgtccttcac ccacgacggg gcgagagcgg tcttgtcggc gcccttcatg aacacgttgg
     6061 agctgatgta ctcccagtcg gcccgcggga cgatgcggtc gcgcaggtcg gcccagccct
     6121 tgtcgaactc gtggttgccc gccgcgctga cctcgaggcc ggcggcgttc agcgcgtcga
     6181 tggtggggtt gtcgtcctgg atgaacgagg tgaacgtcga agcgccgacc aggtcgccgg
     6241 cggcggcgaa gatcgtgttc gggttcttgg cgcgcacgtc gcggacggca ccggcgagca
     6301 ccgcggcgcc tgccgcagca ccatcggcct cgatgcgacc gtggaagtcg ttgatcgtgg
     6361 cgatgtcgat gctcaccggg ggcgccgtgg ccgagacgcc gacgaggatc gggtcgtggt
     6421 cactggagcg gaacggggtg cccgcctcgg tggcgccgaa cgcgtaaccg cggtcgctcc
     6481 actccggcga gttgatgccc cacacgccgg cgccggtgac cgacgcggcg agcgacggcg
     6541 aggcgatgac gtggtcgagc gagccgagct cgccgtcgaa ggagtaggtg tactgcccct
     6601 tcgccttgga cggcacggtg tcgagccagc cctgcgacgt gaacacgtcg atcgggtcct
     6661 cgtggccgta ggcgttgaag tcgccgatca gcagcatgtc gtcgctgccg ctgtcggtct
     6721 tcagcttctc ggtgaatgcg agcagggact tcgcctgcgc gacgcggtcg gcgttgaaga
     6781 agccctggcc gtcggccggc tgcgtgccgg tgccggactt ggacttgagg tggttggcga
     6841 cgaccgtgac ggtgcgtccg tcgatgtcga aagcctgggc gatcggctcg cgggcgttgc
     6901 cccagacggt ctcgtcgatg atggtctgcg ccgcgccctt gggcgtgacg gcggccttct
     6961 tgtagatgat cgcgctggtg atgtagtcgg tgatcgcggc gtcgtcgagc gccttcggcg
     7021 tcggcacata ggcccacacc tcggagccgg cggcggcgtt cagtccgctg acgagatcgg
     7081 ccagcgcggt gtcgagcggc tcgccgagct tgggcccgtt ctcgatctcc atcagcgaga
     7141 cgatctcggc gccggtgccg ttgatggccg cgacgatctt cgacttctgg atcgcgaact
     7201 gagcggcatc cttcgcaccg cgcgcatcgg ggttctcaga gctgagcgtc gtgaagtagt
     7261 tgtacacgtt gaacgacgcg atctgcacgt cgccgccgac cgacgggacg gacggacgcg
     7321 ggttgttcgt cttgaacgtg accttctcgg aggccgggga ggtgctgttc agcggcgtga
     7381 ccggctgcag acgccagtcg tcgtggtcgt actgcagcac gaagcccttg ccgccgaagt
     7441 ccacggcgtc gccgttgcgc accacgacgt ccttggtgaa gtagggctgg tcgccggtgt
     7501 ggccggcctt gccgatcccc acgctgtaac cgtcgtcgag caggatgcgg tcggcacggt
     7561 tggccgcggc gatggcgtcc gcctcggcac ccgggcgctt cagctccgtg ctcttgacgt
     7621 tgagctcacc cgggttcagc cacagggtgc cgtagttgta cagctggtgg ctggaggcga
     7681 ggaggtagtc gccagcgggg gcgacgagca tgttctcgta ctgctcgcgg tcggtgccga
     7741 cgacggaggt cggcagcgcg acgggtgcgg ggacgccgac accggccgtg acgacggaga
     7801 cgtcgctcgc agaggccggc gtgatctgcg tctgaccgta gtactcgctg acggcgccgt
     7861 tgaccgagac caggtcgccg atcttgccgg ggacggtctt gccgttcagg tagacgaaga
     7921 cgccgtcgga cttgcccgcg gtcttggtct cgccgccggt gccctgtgtc tggatcacga
     7981 cgccgttgta gccgccgacg cggtagtcgg cggtgatgac gccctccacg gtgacggtct
     8041 tgccgaccag cggcgaggtg tcgcccgtgc cctgcacgtc ggcgatggag accttctcac
     8101 ccggctgcgg ccccgggtcg gtgccggggt cggtcgcgcc cgtctcgccc ttggagttgg
     8161 tcggagagac ggacgccgac agcgagaagt cgtccttgtt gctgtcggtg tccttgccgc
     8221 cggcgcggtt cagggacttc acgtcggtgt tgctcgaggg agccgtggcg actgcgccct
     8281 cgaaggtgtt cgaggagccg tagccgagca ggtcgatcac gccttcggcg ccagcggcgt
     8341 tccccgcggc cagcgtgagg ggtgcggtgc ccttgacgag cgcgacggtg ccggtggtgc
     8401 cggacgggtt cagcgcggtg ccggtcacat cggccgtcgg cagcgccgga gcggtcgacg
     8461 tgccgctgtt ggtgccgccc tggacgagga agtagccgtg cgcgggaatg gtgccggaca
     8521 gagcgacgac tccgccgaag ctgcccgtgc ccgtcgcgga gcggtactgc agcgacatgc
     8581 cgtcgagcga cacggccgcc gccgtagggt tgtacagctc gacgaacttg ttcttgaagg
     8641 ctgcgccggc gctgccgccg gacaggtacg cctcgttgat gaccacgccg gtgccgtcgg
     8701 tgctcgcgaa tgcgggcgat gcggcgagcg agccgaagcc cagcgcgaac acggtggtca
     8761 tggcgagggc gcatgtgcgc cctcggtttc tgtgctgtgc gagcggaacc gtccggttct
     8821 cgtcgccggg ggcggacatc ttcactgctg tctcctcggt cagggcaaag agcgcaggtc
     8881 tcgtggacct gcgcgacgtg tgcaccgggc gcacacgcat ggttgcgacg atagggctcg
     8941 cctccgacat ccggcaagac gattcgccgt tgttggagct tcggtcatcc gatggtcacc
     9001 gaatggccag ccgcggatgg tcgttcctcc cgccgaggag ctcaggacgc ctgttcgtcc
     9061 tcgggcgcgg ccgccggctc tcccgcgttc gcggcgtcct cgaactcctc gacctcgcgc
     9121 tcccaggcat ccttcgccag tcggtaccag aggtagaagg cgaagccggc gaacacggcc
     9181 cactcggcgg cgtagaagat gttcagccag ttgatcggcg actgctcctg cggcggtggc
     9241 gagctgatgt cgacgaggcc ggccgacgcc gtggcggatg ccaggtacgg gcggtacacg
     9301 tccagcccgt cgatgtcgtg ccagcggctc agcagcgccg cgggcgacat ccggttgagc
     9361 tccatcggat cggagcgcgg aggcacctcg acaccttcgt cgtcgatcag ccgtccggtg
     9421 atctcggctg tgacggcgcc ctgcttctcc agcgctgcga cggcggcatc cgcctgcttc
     9481 ctggtggcgg cccagccgac cgcgacggcg atcgaggtac gatcggcgac ccggagctga
     9541 ccggtgaccc agtagccctc ggtgccgtcg ttgatgcgct gcgagatgac gatgaagtcc
     9601 ccggcgaccc acgtccccga ggtccggacg cgctgcccgc cgaggctctg gtcgaggtac
     9661 ttccccggct cgatcacgtc ggtcagcgga cgcaccttct cggttgcgcc gggcgcgggg
     9721 ggcgtcgtgt cgatggctcg ctcgagctgc cactggccga gccatgcgaa cacgcccgcg
     9781 acgaccaggc acagcgcgag gacgccgatc caccgcctgc gcagcatgac ctcgcgcagg
     9841 gtgggcggga actcctgcgg cgcgagcggc gcgctgttcg tcatcccagc tcgtagggcg
     9901 cgagcacgac ctcgacgcgc tggaactcct tcaggtcgga gtatccggtc gtcgccatcg
     9961 acttgcgcag agcgccgatc aggttggcgg tgccgtccgc gacgggcgcg gggccgtaga
    10021 ggatctcctc gaggttcgcg acgccgtcca cggcgacgcg gcgcccgcgg ggcagctgcg
    10081 ggtggtgcgc ctcggcaccc cagtggaagc cctggc
//
LOCUS       DATMSX010000474         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149040,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000474 DATMSX010000000
VERSION     DATMSX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149040"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VN041_15090"
     CDS             <1..1153
                     /locus_tag="VN041_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division initiation protein"
                     /protein_id="HWU30408.1"
                     /translation="VAGNDARLARLEDELAAARAQAEDAAAQVSTLATELSDAPKGAD
                     EPQSRQQFEAVLRVAEDQASVLIHNAATQAERLLQAAREEAESKRAELAADVARITAQ
                     AEHDADQVRLKIDTELTAHQARLERESSHAAEKVTQAEREAATVRTEAEKGAAALRAM
                     VTRETTDQRSDAEREVREMNARVLEFEETLTRRQDDAQQEFLVLHNQAVAHAERITTD
                     ANEQVAASLEHAQRISAKAEDYERLMRSQAQAIEAEAQVRSRETLDRARVKAQKIIDS
                     VTGHATAALRDAEDRTRQLRWQQQQLNSFMAEVRELIRPEGVLAKPASAPAPDAAAPE
                     VEADAEPDDALEAELDDLIEADATDEDEADSDEDDVEEAELDEDETARA"
     gene            1372..1797
                     /locus_tag="VN041_15095"
     CDS             1372..1797
                     /locus_tag="VN041_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWU30409.1"
                     /translation="MAQRNTWQRERVRDALTDARGFVSAQTLHADLRQANTGIGLATV
                     YRALAGLAASGEADSLQSPEGEALYRACSTQGHHHHLICRSCGRAVEIEAKDVEQWAQ
                     RTAAAYGFTQAEHVVDIFGLCAECAARLASETEPAGAGE"
     gene            1794..2849
                     /locus_tag="VN041_15100"
     CDS             1794..2849
                     /locus_tag="VN041_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWU30410.1"
                     /translation="MTSAPSRMPETASSGAVHAHRHPPRRIPTGRAIGIGVGILAVLV
                     LIDVFVPNLFTKALPDRAQDGLTLTLSVLIEALPWVILGVVLSIVVQVWLPADAVQRW
                     LPQNAWVRRAVLSLLGMFIPVCECGNVPFARGLMMRGLAPAEALTFLVAAPIVNPIVI
                     LTTHAAFGWDGGILIARLVGGYLIANLIGWIYSRHPDPQSLLTERFVETCEQVVHEPG
                     SPTRRSLIQFLVELRAVMPALVIGSALAGAVQVLVPRDVLLAIGSNPVLSILAMVALA
                     MTVAICSNVDAFFALSFASTFSSGAIIAFLLVGPLVDVKMLALLRTTFVARVLAGLVG
                     LVVLSACAIGIGVNVFG"
     gene            2854..3621
                     /locus_tag="VN041_15105"
     CDS             2854..3621
                     /locus_tag="VN041_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HWU30411.1"
                     /translation="MDEPRRLHALASRWLGLGLATVVAVVTLGLAVAGRLTMYISPDT
                     VWFACLAAVVMLAAAIWSCTLPLGVEGDHDHTHGASGPDAAAHRRRVLQTAGVVAGGI
                     VSSAVVLAALLLPPASLSAQLAVSRAGENPVLFAGADDVTLGVADTTTFGVGDWASAF
                     ATSTRPEEYDGASVTLTGFVTPGKGGEAGLTRMIITHCVIDAQPATVPVGGVAGKYST
                     GQWIEVTGTVRAEADGTLRIEPTSVKKVAEPKDPYEY"
     gene            3611..>4184
                     /locus_tag="VN041_15110"
     CDS             3611..>4184
                     /locus_tag="VN041_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30412.1"
                     /translation="MSTDGEAPLTRAQLRALRAAAEAAEPAQDPEAAGAAEPSAAEHS
                     EGDPGATAARAEESWRAATSEEATAAPPPAPPAAPPTIRHPVPGIVPRPAWPAPVSQH
                     PEQPFPTPATTDAADLPGHTPGADTPDVGPIRGIRPESGVSGPDLGPAHPADPVIDEN
                     ASDENASEDAPPADRHPLPAIPAPTSPITTG"
BASE COUNT          596 a         1519 c         1488 g          581 t
ORIGIN      
        1 cgtcgccggc aacgacgccc gcctggcccg gctggaggac gagctcgccg ccgccagggc
       61 gcaggccgag gatgccgccg cgcaggtgag caccctcgcg accgagctga gcgatgctcc
      121 caagggcgcc gacgagccgc agagccgcca gcagttcgag gccgtgctgc gcgtggccga
      181 ggaccaggcg agcgtgctga tccacaacgc cgccacccag gccgagcgcc tgctgcaggc
      241 cgcacgcgag gaggccgagt ccaagcgagc cgagctcgcc gccgacgtcg cccgcatcac
      301 cgcgcaggcc gagcacgacg cggaccaggt gcgtctgaag atcgacaccg agctgaccgc
      361 gcaccaggcg cgtctcgagc gcgagtcctc gcacgcggcg gagaaggtca cgcaggcgga
      421 gcgcgaggcc gcgaccgtgc gcaccgaggc cgagaagggc gctgccgcgc tgcgcgccat
      481 ggtcacccgt gagaccaccg atcagcgatc ggatgccgag cgcgaggtcc gcgagatgaa
      541 cgcgcgcgtg ctggaattcg aggagaccct cacccgccgt caggacgacg cgcagcagga
      601 gttcctggtg ctgcacaacc aggccgtcgc gcacgccgag cgcatcacca ccgacgccaa
      661 cgagcaggtg gccgcgtcgc tcgagcacgc gcagcgcatc tcggcgaagg ccgaggacta
      721 cgagcgcctg atgcgcagcc aggcgcaggc catcgaggcc gaggcgcagg tgcgctcgcg
      781 cgagaccctc gaccgcgccc gcgtcaaggc gcagaagatc atcgactcgg tcaccggcca
      841 cgccaccgcc gcactgcggg atgccgagga ccgcactcgc cagctgcgct ggcagcagca
      901 gcagctgaac agcttcatgg ccgaggtgcg cgagttgatc cgtcccgagg gagtgctggc
      961 gaagccggca tccgctccgg cgccggacgc cgccgctccc gaggtcgagg cggatgccga
     1021 gcccgacgat gccctggagg cggaactcga cgacctgatc gaggccgatg cgaccgacga
     1081 ggatgaggcc gactccgacg aggacgacgt cgaagaggcc gagctcgacg aggacgagac
     1141 cgcccgcgcc tgaccgccgc gtgctgcggg tgcccccggc cggctgtagt ggcgccgcct
     1201 gtctcagaac tggcgggaga ggcgggctgc gacccgctcg ttgtggcaca cgaatgcgcg
     1261 cgtgtctcag aaagggcggg tgcgtgccgc atccactcgc cgtttctgag acacgcgccc
     1321 tcggacgatc gtctctgagc ggctcacagg cgcccggcct agactcgagg catggctcag
     1381 cggaacacct ggcagcggga gcgcgtgcgc gatgcgctca ccgacgcgcg cggtttcgtg
     1441 agcgcgcaga ccctgcacgc cgatctgcga caggccaaca ccggcatcgg cctggccacc
     1501 gtgtaccggg cgctcgccgg gctcgcggca tccggtgagg ccgactccct gcagagcccc
     1561 gagggcgagg cgctgtaccg cgcctgctcc acccagggac atcaccacca cctgatctgc
     1621 cgctcctgcg gacgcgccgt cgagatcgag gccaaggacg tcgagcagtg ggcgcagcgc
     1681 accgccgccg cgtacggctt cacgcaggcc gagcacgtcg tcgacatctt cggcctgtgc
     1741 gccgaatgcg ccgcacggct cgcgagcgag acagagcccg caggagcggg cgagtgacct
     1801 cagccccgtc ccggatgccg gagacggcat cctccggtgc ggtgcacgcc catcggcatc
     1861 caccccggcg cattcccacg ggacgggcga tcgggatcgg agtcggcatc ctcgcggtgc
     1921 tggtgctcat cgacgtcttc gtgccgaatc tgttcacgaa ggcgctgccc gaccgcgctc
     1981 aggacgggct caccctcacg ctcagcgtgc tgatcgaggc gctgccgtgg gtgatcctcg
     2041 gcgtggtgct gtcgatcgtg gtgcaggtgt ggctgcccgc ggatgccgtg cagcgctggc
     2101 tgccccagaa cgcctgggtg cgtcgcgccg tgctgtcact gctgggcatg ttcatccccg
     2161 tgtgcgagtg cggcaacgtg cccttcgccc gtggactgat gatgcgcggg ctggctccgg
     2221 cggaggcgct gacattcctc gtggcggctc ccatcgtgaa cccgatcgtg atcctcacca
     2281 cccacgcggc gttcggctgg gacggcggca tcctgatcgc gcgcctcgtg ggcggctacc
     2341 tgatcgcgaa cctgatcggc tggatctaca gccgccatcc cgacccgcag tcgctgctca
     2401 cggagcggtt cgtcgagacc tgcgagcagg tggtgcatga gcccggttcg ccgacccggc
     2461 gcagcctgat ccagttcctg gtcgagctgc gtgccgtgat gccggccctc gtgatcggct
     2521 cggcgctggc cggggcggtg caggtgctcg tcccgcgcga cgtgctgctg gcgatcgggt
     2581 cgaaccccgt gctgtcgatc ctggcgatgg tggcgctcgc gatgaccgtg gcgatctgct
     2641 cgaacgtgga cgcgttcttc gcgctgtcgt tcgcgtcgac gttctcgtcg ggcgcgatca
     2701 tcgcgttcct gctggtcgga ccgctggtcg acgtgaagat gctcgccctg ctgcgcacga
     2761 cgttcgtcgc gcgagtactg gccgggctgg tgggcctggt cgtgctcagc gcctgcgcca
     2821 tcgggatcgg ggtgaacgtc tttggctgat cacgtggacg agccccgccg cctgcacgcc
     2881 ctggcctccc gctggctcgg gctgggcctt gcgaccgtgg tcgcggtcgt gacgctcggg
     2941 ctcgccgtcg ccggacggct gacgatgtac atcagccccg acaccgtgtg gttcgcgtgc
     3001 ctggcggcgg tggtgatgct cgccgcagcg atctggtcgt gcaccctgcc gctcggcgtg
     3061 gagggcgacc acgaccacac tcacggagcc tcgggcccgg atgctgcggc gcatcgccgg
     3121 cgggtgctgc agacggcggg cgtcgtggcc ggcggcatcg tgtcgtcggc tgtcgtgctc
     3181 gctgcgctgc tgctgccacc ggcatccctc tccgcccagc tggcggtgtc gcgcgcgggg
     3241 gagaacccgg tgctcttcgc gggtgcggat gacgtgacgc tcggtgtcgc ggacaccacg
     3301 acgttcggcg tgggcgattg ggcgagcgcg ttcgccacct cgacccgccc cgaggagtac
     3361 gacggcgcct cggtcaccct gaccggtttc gtcacgccgg gcaagggggg agaggccggc
     3421 ctcacccgca tgatcatcac gcactgcgtg atcgacgcgc agcctgccac cgtccctgtc
     3481 ggcggtgtgg cgggcaagta ctcgaccggc cagtggatcg aggtcacggg caccgtgcgc
     3541 gcggaggccg acggcaccct gcgcatcgag ccgacgtcgg tgaagaaggt cgccgagccg
     3601 aaggacccct atgagtactg acggcgaggc tccgctcacc cgggctcagc tgcgtgccct
     3661 gcgtgccgcg gcggaggcgg ctgaacccgc acaggacccg gaggctgccg gcgcggccga
     3721 gccctccgct gccgagcact ccgagggcga tccgggtgcc acggcagccc gtgcagagga
     3781 gtcctggcgt gccgcgacgt ctgaagaggc gaccgctgca ccgccgcctg cacccccggc
     3841 tgcgccgccc accatccggc atcccgtacc cgggatcgtc ccccgcccgg cgtggccggc
     3901 tcccgtctcg cagcatccgg agcagccgtt tccgacgccc gcgaccaccg acgcagccga
     3961 cctgcccggt cacactccgg gggccgacac gccagatgtc ggaccgattc gcgggattcg
     4021 tccggaatct ggcgtgtcgg ggcccgatct ggggcccgcc catcccgccg accccgtcat
     4081 cgacgagaac gcgtccgacg agaacgcgtc cgaggatgcg ccgcccgccg accggcatcc
     4141 gctcccggcg atccccgctc ccacgtcgcc catcacgacc ggcc
//
LOCUS       DATMSX010000475        17511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_168101,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000475 DATMSX010000000
VERSION     DATMSX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168101"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VN041_15115"
     CDS             <1..999
                     /locus_tag="VN041_15115"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWU30413.1"
                     /translation="VLAAAGPLALLALRRLPGPTVAVVAALALLDVLTTPVGGPPYIA
                     LAFAVIGAVARGAIVWAAISVGVGWASAILIGTLIDRTWFPGVVVAATVALAACFAIG
                     TGLRSRGARRAAMHAEIQHRRRSAEEQERVRIARELHDVLGHALSQMNVQAGVGLHLF
                     DRDPEQARAALQNVKDTSKLALEEVRGVLGVLRDGEVPLVPQAELAELPRLIAGITAP
                     GLEVALDDRLEGQVPGRAAQFAAYRIVQEALTNVVRHADATHARVVLDRREASLIVTV
                     SDDGAGFGGVDPATAGHGGVLGMRERAELLGGGIRFSDRPGGGAEVVAELPWGRTS"
     gene            996..1658
                     /locus_tag="VN041_15120"
     CDS             996..1658
                     /locus_tag="VN041_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30414.1"
                     /translation="MIRVALADDQLLVRAGFRALLDAEDDIEVVAEAAGGRELLDRIR
                     SAPADVVLMDIRMPDGDGLWATEQIAEDPALQDVHVVIVTTFELDEYVARAVRAGAAG
                     FLVKDTEPADLVRAVRAVAEGDALLSPGVTRRLLDRLAVGLREAPDAAQLDVLTERER
                     EVLRLVGLGLSNDEIAAKLFLSPLTAKTHVSRILSKLQARDRVHLVVIAYETGLVAPG
                     WQ"
     gene            1784..2095
                     /locus_tag="VN041_15125"
     CDS             1784..2095
                     /locus_tag="VN041_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30415.1"
                     /translation="MLAATAAVAAHAGWGVAPFWPFFFIGPVMFLIIVALLITLIVRR
                     RRYWGGPWQAAPGGGAPWMRGAQSRSAEQILADRFARGDVDETEYRARLEVLRANRPE
                     A"
     gene            2327..2671
                     /locus_tag="VN041_15130"
     CDS             2327..2671
                     /locus_tag="VN041_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU30416.1"
                     /translation="MGKFIYESAHKVEIEDRALLHMQLVITAKLRRSEPFPFSWREDV
                     SVGGGRTTVWMHANSALVFKYYGSRQPSVNRAWIETLAFTANSPGGLYLTPEPASTGP
                     IQRETADADAFA"
     gene            complement(2795..3562)
                     /locus_tag="VN041_15135"
     CDS             complement(2795..3562)
                     /locus_tag="VN041_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWU30417.1"
                     /translation="MEPGAVKSAGRALEIIEHLAAVEAQTFPQLLKALELPRSSAHGL
                     LRTLVAAGWVELDPATKRYTLGLKAWQIGQRYDGHRLLLHAGPSLMAKLTDETGETVQ
                     MARLDGVENVYIAISPSPNPMRLASNVGMRLHAHATGIGKALLSTLDDDDVLRRLHQV
                     AIPRLTARTLTQPDEILSAIRRGRDLGFFVDDEEFIDGCRCVAIPITTTEETGVAAAM
                     SITMPTSRTDDLWPYSMVEPLRRTVQHLRADMALELA"
     gene            3696..5039
                     /locus_tag="VN041_15140"
     CDS             3696..5039
                     /locus_tag="VN041_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30418.1"
                     /translation="MSSQSSTRPELAAIVRKVTLRLIPILLLMYILAFLDRANLGFAK
                     VAWQADTGISDAAYALGAGIFFVGYAIFEIPSNLLMRRVGAKIWLARIMISWGTVSSL
                     MIFATNEWAFYILRVLLGITEAGFFPGVILFLTYWIPLKYRARVNGLFYFGAPLAFIF
                     GGPLSGMLLDLDDKVAIHGWQLMFLVTGILTVLVGIVAFFYLDNGPKDAKWLNGHERE
                     ALLSALEDENTAKENHSPRGALRALGNPVVLYFAAIYLTIQMSVYGVTFYLPTQIATI
                     VGQPVGLEVGLLTAVPWTFALIVTFVLSRLADSTGQRRLIATLALAAAGVGIVLSAVI
                     QDPIVALAALSVGAAGFISVQPVFWTLPTSFLLGAAAASGIALINSLGSLGGFLAPIL
                     KNWADATFGDSAGLIVLAVIALLGAALIALSRVVSPAVRETDGVGLAASAPTHQR"
     gene            5072..6109
                     /locus_tag="VN041_15145"
     CDS             5072..6109
                     /locus_tag="VN041_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWU30419.1"
                     /translation="MRELVLSEIGRLDVRDAPTPHPGPDEVLIRVSATGICGSDVHGY
                     TGENGRRHPGQVMGHESSGTVAAVGADVDGLVEGAPVTFNPVVVPADDAAEYAGREQH
                     HPRKYVIGVAAEIPAAFADYVVVPARNVVPLPDSLPIELGALIEPLAVAVHAVRKLPT
                     DSLRRVLVIGGGPIGQSVVIALRDAGAGTILVSEMDAARRELVQKLGAEVIDPADGPL
                     SEQLRERGGLADAAVDAVGITPTLQDALTSTVLGAPVCLVGMGAPQVSLDAFLISTEE
                     RSLIGSFTYGSDDFTHAAHTIAGDPDVYRVLISREIDVADADNAFAGLAAGDGTPGKV
                     LVRFDRDGETR"
     gene            6106..7284
                     /gene="rhmD"
                     /locus_tag="VN041_15150"
     CDS             6106..7284
                     /gene="rhmD"
                     /locus_tag="VN041_15150"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HWU30420.1"
                     /translation="MTGTPTIREVRAYTVRGGGADYHDQNAGHWIDDHIATPMSVYPE
                     YRQSRQSFGINVLGTLVVEIEASDGTVGFSVTTAGEIGAFIVEKHLARFLEGRAVTDI
                     ERIWDQMYKATLYYGRRGVVLNAISGVDLALYDLLGRIRQVPVFELLGGPVRDELQFY
                     ATGARPDRAKELGFIGGKMPLHHGPAEGEEGLRKNIELLADMRERVGDDFWLMYDCWM
                     SLDVDYATRLAHAASDHGLKWLEEALIPDDYWGYAELRKRAPSSMLITTGEHEATRWG
                     FRQLLETGVDLIQPDVGWCGGITELIKISALADAHGTMVVPHGSSVYSYHFVVTCTNS
                     PFAEFLMMHPTAEEVVPMFSPMLTGEPVPVNGRLKVPETPGFGVELSDAIQKHRPYTH
                     "
     gene            7300..8154
                     /locus_tag="VN041_15155"
     CDS             7300..8154
                     /locus_tag="VN041_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU30421.1"
                     /translation="MEFLRLGDPGAETPAVRHEGRVLRLDGLTGDIDGAFLAGGGIAR
                     TRAALDAGELPSFEGAELLRVGAPIARPQAVLCIGQNYAAHAAESGDAPPSVPILFHK
                     HPNTVVGPYDDVLIPPGAEKVDWEVELGVVIGTRARYLASPEDALAHVAGYVVSHDVS
                     ERAFQVEQSGGQWSKGKNAETFNPLGPALVPAEEVDPQNLRLWSTVNGEPRQDSNTAD
                     MIFTVTQIVHHLSQYLVLEPGDLINTGTPQGVALSGRFPYLRAGDVVEIGIDGLGSQR
                     QTLVPAER"
     gene            complement(8714..11686)
                     /locus_tag="VN041_15160"
     CDS             complement(8714..11686)
                     /locus_tag="VN041_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HWU30422.1"
                     /translation="MPAFEQAITEADVVFIVGTGLSAATTNGAPTSTWVGLVRSGAER
                     AAKLNNRVDEQWLTIVQSQLDYPSTDMVINAASSVASAIRELGDTAYAAWLREDVGDL
                     VASDEAVASALLSYPFPVLTTNYDKLLEQVGGRETRDWTDSRGFQDVVTRSSSAIGHI
                     HGVWDRPDSVILTAADYARLSTHESMQALEKAMATLKSIVYVGFGSGLGDPNFASLLE
                     WHRRVFPQSSTTHYRLCRTDELDELRRLHANDHVVPVAYGDQHADLAPFLARHAPASE
                     SLITNAVGLARDVVQEARELIRDSMTNESVLVDTGADDLVRRDLTIPPILLPVPHATF
                     VRDRLRRGDTAEVERLDPHREIESHDFFVVVGDEGSGLTTAVKWLATQSSELLGTAAP
                     IYVRFSECRHRSDPLGAATRNAAMNCGLIHDRGASLPDHIIAIDDFRASVPRLSDTVL
                     GQVVASDAIVKIIGCHQGEEDELATKLNSLGVSPRILFLGRMRKTDVKNLAERLAPGQ
                     GNRLADEALKVLEAEGLRRTPLTVSLLLYLALRGSAHDTRNQTSMLDAYTLLLLGIGN
                     PHENTTGLTETDLEAVLGKFAESLIWDEKPSLSEPEAVRVIADVIQKFGWQANATHVL
                     DFFLQRRLLRRHGDSIEFARYAYFTLFAAKRALVDPEFRDLVVNDLFYYQPVAVKLSA
                     LSRADEDLLQRIRPLIDEELAVAVSPGSPYEPTPLITIDELPPPEEVHRSTDALERVE
                     DGDDLEFPESDSVGSFGLVKASMSPPARLHRVLTLISTVIRDLDQIENLELKKELLVD
                     TVELWGRFITVLSSDSSLGDLRSAVARHLETEGGTDGDDNADLVDFLSRSIPAGTVIG
                     GMEMTLSSPKLASVLESAINAGELSRTNERLTATLLLLFLQRSPGWAKKAASLIEEAE
                     PTWVVTHFFHALCQDAYAQGATPEEDALHLCKTVYLKQQSFASPDIRSAHLDRYAHRL
                     RTMRAKNKYSPPTSHTDT"
     gene            complement(13417..13489)
                     /locus_tag="VN041_15165"
     tRNA            complement(13417..13489)
                     /locus_tag="VN041_15165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13454..13456),aa:Phe,seq:gaa)
     gene            complement(13558..13631)
                     /locus_tag="VN041_15170"
     tRNA            complement(13558..13631)
                     /locus_tag="VN041_15170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13595..13597),aa:Asp,seq:gtc)
     gene            complement(13658..13730)
                     /locus_tag="VN041_15175"
     tRNA            complement(13658..13730)
                     /locus_tag="VN041_15175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13694..13696),aa:Glu,seq:ttc)
     gene            13973..15349
                     /locus_tag="VN041_15180"
     CDS             13973..15349
                     /locus_tag="VN041_15180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWU30423.1"
                     /translation="MNEQVKADAAASEECGCAPTPAERRAFWPSAQVTRRGAITVGAL
                     SAIALTAFGVTAGTNAAHAEPGYPSWDDVQRAKANEAAKGAEVTRIEGLIQTLTQQAA
                     AAEQAARVAGDEYYAAQQAYFEAADRADSLQSQADEQAKIADESSRKAAQVATQLYRT
                     GGDQAALELFLSKSSNGADDLLSRLGSMDKLLEYNRTVADTAAAARDSAQHLSDQAKS
                     ARDERDRLQKVAQDKMVKAQAASDAAQAALDEKQNNLQTLQAQLAALKDTTTATVAGY
                     QQGEAVRKAREAAEAEARRKAAEEAAAKNHNNGGGGGGGGGTGGSGSGAWRRPSGGRI
                     TSYYGRRNVQCTSGYCSSREHGGIDIANGCGAPIYAAHSGTVDFAGYNGGYGNYTRIN
                     HGGGVGTGYGHQSQFAVRRGQYVNAGQVIGYAGNTGNSFGCHLHFEVYVNGSRVDPYP
                     FMTARGAL"
     gene            complement(15462..15953)
                     /gene="ppa"
                     /locus_tag="VN041_15185"
     CDS             complement(15462..15953)
                     /gene="ppa"
                     /locus_tag="VN041_15185"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWU30424.1"
                     /translation="MGAHDAVIEIPRGSRVKYEVDHETGRVHLDRVLYTTFGYPADYG
                     YFDNTLGEDGDPLDVLVLLDHAIYPGVVVNVRPVAVLKMSDEAGGDDKLVAVLSKDPR
                     WAHIQDVDDLAEYTKKEIAHFFEHYKDLEPNKWVKVDEWGDAAEAQRILDEAIARFGA
                     EGH"
     gene            complement(16071..16931)
                     /locus_tag="VN041_15190"
     CDS             complement(16071..16931)
                     /locus_tag="VN041_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30425.1"
                     /translation="MSRGDSVFVIALLVAALFTGCTPTPPPASPKPSPHASSSPTPAA
                     DAIRAVDFGSMTFTWDTLGVQYPVKLSGGRAEVEDPYYKQTALFQLGEPTFADANGDG
                     LIDAAVPLQFESGNGIMNNWFIWLAQADAPDQPQQIPFPIASGARCADRVTGVEAIAG
                     GFRVHEVMRNSFEATGPCAQSGTMERTRDVGAAGDGTGAGSWPVTLDGQGFGGYCPVK
                     VYTEGDFAQAQGRVGPSDDAPQTSATGEKLYTPIQHYPFLEPDGWHLTGFLPDQQTDD
                     SVVCVWVPKA"
     gene            17029..>17511
                     /gene="tilS"
                     /locus_tag="VN041_15195"
     CDS             17029..>17511
                     /gene="tilS"
                     /locus_tag="VN041_15195"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWU30426.1"
                     /translation="MPSLDPAVAEVRRAVRATLAALEAGPVVVALSGDADSLALTAAT
                     VFEARALGLPVTAAIVDHGLQPGSDAVAQRAADQARELGADARVLSVHVDQQHPDGLE
                     AAARAARYNVLRQCAAELGASAVMLGHTLDDQAETVLLGLARGSGAASLQGMAQLRED
                     A"
BASE COUNT         2866 a         5849 c         5789 g         3007 t
ORIGIN      
        1 gtgctcgccg ccgcggggcc cctggcgctt ctggcactac gcaggcttcc cggaccgacg
       61 gtcgcggtcg tggcagcgct cgcgctgctc gacgtgctca ccaccccggt cggcggcccg
      121 ccgtacatcg cgctggcgtt cgccgtgatc ggtgcggtgg cccggggcgc gatcgtgtgg
      181 gcggcgatct cggtcggcgt cggctgggca tccgcgatcc tgatcggcac cctgatcgac
      241 cgcacctggt tcccgggcgt ggtcgtcgcg gcgacggtcg cgctcgccgc gtgcttcgcg
      301 atcggaacgg ggctgcgctc ccgtggagcg aggcgcgcgg ccatgcacgc cgagatccag
      361 catcgccgcc gcagtgccga ggagcaggag cgggtgcgca tcgcccgcga gctgcacgac
      421 gtgctgggtc acgccctgtc gcagatgaac gtgcaggcgg gcgtggggct gcacctgttc
      481 gatcgcgatc cggagcaggc gcgcgccgcg ctgcagaacg tgaaggacac ctccaagctc
      541 gcgctcgagg aggtgcgcgg cgtgctcggc gtgctgcgcg acggggaggt gcccctcgtc
      601 ccgcaggccg agctcgccga gctgcccagg ctgatcgccg gcatcaccgc gccgggcctg
      661 gaggtcgcgc tcgacgaccg cttggagggg caggtgcccg gtcgcgctgc gcagttcgcc
      721 gcctaccgga tcgtgcagga ggcgctcacc aacgtcgtcc ggcacgcgga tgccacgcat
      781 gcacgggtcg tgctggatcg gcgcgaggcg tcgctgatcg tcacggtcag cgacgacggc
      841 gccgggttcg gcggtgtcga cccggcgacc gccggccatg gcggcgtgct cgggatgcgt
      901 gagcgcgcgg agctgctggg cggcggcatc cgcttctccg atcgacccgg cggtggtgcc
      961 gaggtcgtgg ccgaactgcc ctgggggagg acgtcatgat ccgcgtcgcg ctggccgacg
     1021 atcagctgct cgtgcgcgcc ggattccgtg cgctgctgga cgccgaggac gacatcgagg
     1081 tcgtcgccga ggcggccggc ggccgcgaac tgctcgaccg catccgatcg gcccctgccg
     1141 acgtggtgct gatggacatc cggatgcccg acggcgacgg cctgtgggcg acggagcaga
     1201 tcgccgagga cccggcgctg caggacgtgc acgtggtgat cgtgacgacg ttcgagctgg
     1261 acgagtacgt cgctcgcgcc gtgcgcgccg gcgctgcggg gttcctggtg aaggacaccg
     1321 agcccgccga cctggtgcgg gcggtgcggg cggtcgcaga gggcgacgcg ctgctctctc
     1381 ccggggtgac gaggcggctg ctggaccggc tcgccgtcgg cctgcgggag gcgccggatg
     1441 ctgcgcagct ggatgtgctc accgagcgcg agcgcgaggt gctgcggctg gtgggcctcg
     1501 ggctcagcaa cgacgagatc gctgcaaagc tcttcctcag cccgctcacg gcgaagacgc
     1561 acgtctcccg catcctgtcc aagctgcagg cgcgcgaccg ggtgcacctg gtcgtgatcg
     1621 cgtacgagac cggcctggtg gcgcccggct ggcaatagcg ctgctgctgc gggtactccc
     1681 gcgggagcat ccggagtcac tccccggggc ggattcgccc ggcgaccgat cggacgacgc
     1741 tggagtcatc ggtcgagaca cggccgatga aaggaagggc atcatgctcg cagcaaccgc
     1801 ggccgtcgcc gcacacgccg gatggggcgt cgccccgttc tggccgttct tcttcatcgg
     1861 cccggtgatg ttcctcatca tcgtggccct gctcatcacc ctcatcgtgc gccgtcggcg
     1921 gtactggggt gggccctggc aggcggctcc cggtggaggg gctccctgga tgcgcggcgc
     1981 gcagagccgc agcgccgagc agatcctcgc cgaccggttc gccaggggcg acgtcgacga
     2041 gaccgagtac cgtgcgcgcc tggaggtgct gcgcgcgaac cgtccggagg cttgagctct
     2101 ccgttcggct gccgagacgt ccgtcccgat atgagatcct cgcttccgcg ggccgtctca
     2161 tgctggggcg gatgtctcct tccggggcgc ccggtcacac tgcgcggcac gagatcggga
     2221 cccattccgg cgtgaagtgt gcgagggtgt ggatatcccc acatgtcgga tttcaggccg
     2281 cacctcttgg caacgggttt ccgtggagaa gaccagagga cgcatgatgg gcaagttcat
     2341 ctacgagagc gcacacaagg tggagatcga ggatcgcgcg ctgctgcaca tgcagctcgt
     2401 gatcaccgcc aagctccgtc gcagtgagcc gttccccttc tcgtggcgcg aggacgtcag
     2461 cgtcggcggc ggccggacca ccgtctggat gcatgcgaac agcgccctcg tgttcaagta
     2521 ctacggcagc cgtcagccga gcgtgaaccg cgcgtggatc gagaccctcg ccttcaccgc
     2581 gaactccccg ggcgggctgt acctcacgcc ggagccggcg tcgacgggcc cgatccagcg
     2641 ggagaccgcg gacgccgacg cgttcgcgta ggcgtcccgg cactcgccgt tcctgctcag
     2701 cgcgagcaat gccgcgtagg tcaggctgaa agcggcctcg gctgccagcg gggcgtctgc
     2761 ttcggtgttc cgccagccct gccgctggag tggatcacgc gagttcgagt gccatgtccg
     2821 cccgtaggtg ctggacggtt ctgcgcaggg gctcgaccat cgaatacggc cacaggtcgt
     2881 cggtgcggct ggtcggcatg gtgatgctca tggctgcggc gacgcccgtc tcctccgtcg
     2941 tcgtgatcgg gatggcgacg cagcggcatc cgtcgatgaa ctcctcgtcg tcgacgaaga
     3001 aaccgagatc tcgtcctctg cgaatcgcag acaggatctc gtccggctgc gtgagcgttc
     3061 gggccgtgag ccgcgggatg gcgacctggt gaagtcgccg caggacatcg tcatcgtcga
     3121 gtgtgctcag cagcgccttc ccgattcccg tggcgtgggc gtgcaggcgc atgcccacgt
     3181 tcgaggccag ccgcatcgga ttcggtgaag ggctgattgc gatgtagacg ttctccacgc
     3241 cgtcgagcct ggccatctgc acagtctcac ctgtctcgtc agtgagcttc gccatgagag
     3301 acggcccggc atggaggaga agccgatgtc cgtcgtagcg ctgcccgatc tgccaggcct
     3361 tgagcccgag cgtatatcgc ttggtcgccg gatccagctc gacccacccc gcggccacga
     3421 gcgtgcgcaa cagtccgtga gcgctcgacc gcggcaactc cagggccttc aacagctggg
     3481 ggaacgtctg cgcctcgacc gcggcaaggt gctcgatgat ctcgagcgcc cggccggcgg
     3541 acttgaccgc ccccggttcc atctcggtca ccctcacctc cgcgctgtgt tgtgtgaaca
     3601 ggagtgttgt gccacactat cgcgcgttca ggtctgagac accaagttca tatacgtgaa
     3661 cgccacgaat acccctcgtc aatggagatg tcccgatgtc ctctcagtcc tccacgcggc
     3721 cggaactcgc cgccatcgtc cgcaaggtca cgctccggct gatcccgatc ctgctcctca
     3781 tgtacatcct ggcgttcctc gaccgcgcca acctcggctt cgccaaggtc gcctggcagg
     3841 ccgacaccgg aatctccgat gccgcatacg ccctcggcgc cggcatcttc ttcgtcggct
     3901 acgccatctt cgagatcccg tcgaacctgc tcatgcgccg agtcggcgcg aagatctggc
     3961 tcgcccgcat catgatctcc tggggcaccg tctcctcgct gatgatcttc gccacgaacg
     4021 agtgggcctt ctacatcctg cgcgtgctgc tcggcatcac tgaagccggc ttcttccccg
     4081 gggtcatcct cttcctgacc tactggattc cgctgaagta ccgggcccgg gtgaacggcc
     4141 tgttctactt cggtgcaccg ctggcgttca tcttcggcgg gccgctgtcc ggcatgctcc
     4201 tggacctcga tgacaaggtc gccatccacg gctggcagct gatgttcctc gtcaccggaa
     4261 tcctcaccgt cctcgtgggc atcgtggcat tcttctacct cgacaacggc ccgaaggacg
     4321 cgaagtggct gaacgggcac gagcgcgaag cgctgctgag cgccctggaa gatgagaaca
     4381 cagccaagga gaatcactcc ccgcgcggtg ccctgcgcgc cctgggcaac cccgtggtgc
     4441 tctacttcgc cgccatctac ctcaccatcc agatgagtgt ctacggcgtg acgttctacc
     4501 tgccgaccca gatcgcgacc atcgtcgggc agcccgtcgg actcgaggtc ggactgctca
     4561 ccgccgtgcc gtggacgttc gcgttgatcg tgacgttcgt gctgtcccgc ctcgccgaca
     4621 gcacgggcca gcggcgcctc atcgccaccc tcgctctcgc ggccgccggt gtggggatcg
     4681 tgctctccgc tgtcatccag gacccgatcg tcgctctcgc cgccctgagt gtcggggccg
     4741 ccggattcat ctcggtgcag ccggtgttct ggacccttcc gacgtcgttc ctgctcggtg
     4801 cggccgccgc gagcggcatc gccctgatca actcgctcgg cagcctcggc ggattcctcg
     4861 ccccgattct gaagaactgg gccgacgcga cgttcggtga cagcgccgga ctcatcgtgc
     4921 tcgccgtcat cgccctgctg ggagcggcac tcatcgcact cagccgagtc gtcagccccg
     4981 cagtgcggga gaccgacggc gtcggactgg cagcatccgc gcccacccac cagcgctgac
     5041 ccccggtcgg caagatcaag gagacctact catgcgcgag ctcgtcctca gcgagatcgg
     5101 acgcctcgac gtccgggacg cccccacgcc ccatccgggg cccgacgaag tcctcatccg
     5161 agtgtccgcg accgggatct gcggatccga cgttcacggt tacacaggcg agaacggacg
     5221 ccgtcacccg gggcaggtga tgggccatga gtccagcggc acagtcgccg cggtgggcgc
     5281 cgacgtggac gggctcgtcg agggcgcccc ggtgaccttc aacccggtcg tcgttccggc
     5341 cgacgacgcg gcggagtacg ccggccgcga gcagcaccac ccgcgcaaat acgtcatcgg
     5401 cgtggcggcg gagatcccgg cggccttcgc cgactacgtg gtcgtccccg cgcggaacgt
     5461 cgttcctctc cccgattcgc tgcccatcga gctcggcgcg ctcatcgagc ctcttgccgt
     5521 cgccgtccac gccgtgagga agcttcccac cgacagcctc agacgagtgc tggtcatcgg
     5581 cggtggccct atcggacagt ccgtcgtcat cgcgctgcgc gatgccgggg cgggaaccat
     5641 cctcgtcagt gagatggatg ccgcccggcg cgagctcgtg cagaagctcg gcgccgaggt
     5701 catcgacccc gccgatggtc ctctctccga acagctccga gagcgcggcg ggctcgccga
     5761 cgccgcggtg gatgccgtcg gcatcacccc cactctgcag gacgcgctga ccagcacggt
     5821 gctcggtgct cccgtctgcc tggtcggcat gggtgcgcca caggtgtctc tcgacgcctt
     5881 cctcatcagc accgaggagc gctccctcat cggcagtttc acttacgggt cggacgactt
     5941 cacccacgcg gcgcacacca tcgccggcga ccccgacgtc tatcgtgtgc tgatcagccg
     6001 cgagatcgac gtcgccgacg cagacaacgc cttcgccgga cttgccgccg gcgacggcac
     6061 cccgggcaag gtgcttgtcc ggttcgaccg agacggagag acccgatgac aggcaccccc
     6121 accatccgcg aagtccgcgc ctacaccgtg cgcggtggcg gagccgatta ccacgaccag
     6181 aacgccgggc actggatcga tgaccacatc gcgacaccca tgagcgtcta ccccgagtac
     6241 cgccagtcac gtcagagctt cggcatcaat gtcctcggca ccctcgtcgt ggagatcgag
     6301 gccagcgacg gcactgtcgg gttctcggtc accaccgccg gcgagatagg cgcattcatc
     6361 gtcgagaagc acctcgcgcg gttcctcgaa gggcgcgcgg tcacggacat cgaacgcatc
     6421 tgggaccaga tgtacaaggc cacgctgtac tacggccgac gcggcgtcgt gctcaacgcc
     6481 atcagcggcg tcgacctcgc cctgtacgac ctgctcggcc gcatccgcca ggtccccgtg
     6541 ttcgagctgc tcggcggccc ggtccgcgac gaactgcagt tctacgccac aggcgcccgc
     6601 cccgacaggg cgaaggaact cggcttcatc ggcggcaaga tgcccctgca ccacggtcct
     6661 gctgaagggg aggagggtct tcgcaagaac atcgaactgc tcgccgacat gcgcgagagg
     6721 gtcggagacg acttctggct catgtacgac tgctggatgt cgctcgacgt cgactacgcg
     6781 acccggctcg cacacgcggc atccgaccac ggtctgaagt ggctcgaaga agctctcatc
     6841 cccgacgact actggggata cgccgagctg cgcaagcgcg caccctcgtc gatgctcatc
     6901 accaccggcg agcacgaggc cacgcgctgg ggcttccgtc agctgctcga gaccggcgtc
     6961 gacctcatcc agcccgacgt cggctggtgc ggcggcatca ccgagctcat caagatctcg
     7021 gcactcgccg acgcacacgg gacgatggtc gtgccgcacg gctcgtcggt gtactcgtat
     7081 cacttcgtcg tgacgtgcac gaacagcccg ttcgcggagt tcctgatgat gcaccccacc
     7141 gccgaggagg tggtgccgat gttctccccg atgctgaccg gtgagcccgt tcctgtgaac
     7201 ggccggctga aggttcccga gacgcccggc ttcggagtcg aactctcgga tgccattcag
     7261 aagcaccgtc cctacacaca ctgatctgga gcacgacaca tggaattcct ccgcctcggc
     7321 gaccccggtg ccgagacccc tgcagtccgc catgagggcc gcgtcctgcg cctcgacggc
     7381 ctcaccggcg acatcgacgg cgccttcctc gcgggcggcg gcatcgctcg aacccgcgca
     7441 gcgctggacg cgggcgagct gccctccttc gagggcgccg agctgctgcg cgtcggcgcg
     7501 cccatcgccc gtccgcaggc ggtgctctgc atcggtcaga actatgctgc ccatgcggcc
     7561 gaatccggtg acgccccgcc atcggtgccg atcctcttcc acaagcaccc gaacacggtc
     7621 gtcggtccgt acgacgacgt tctcatcccg ccgggagccg agaaggtcga ctgggaggtc
     7681 gaactggggg tggtcatcgg cacccgtgcc cgctacctcg ccagtcctga ggacgcgctc
     7741 gcgcacgtcg caggctacgt cgtctcgcac gacgtctcgg agcgcgcgtt ccaggtcgag
     7801 cagtcgggcg gtcaatggtc gaagggcaag aatgccgaga cgttcaaccc gctcgggccg
     7861 gcgctcgttc ccgctgaaga ggtcgacccg cagaacctgc gcctgtggtc cacggtcaac
     7921 ggtgaaccgc ggcaggactc caacaccgcc gacatgatct tcaccgttac gcagatcgtg
     7981 catcacctgt cgcagtacct cgtgctcgag ccgggcgacc tcatcaacac gggtacaccc
     8041 cagggcgtcg ctctatcggg tcggttcccc tacctgcgtg ccggggatgt cgtcgagatc
     8101 ggcatcgacg gactcggctc ccagcggcag acgctggtgc ccgccgagag gtagagaccc
     8161 tcgcccatcc gattcgccgt ggtcgacgca ggcgggcgtc accgaggtat ggtgtgccgc
     8221 ccgcctgctt cagcgaacgc gtcgattgcc ctgaggacct cggtgcttcg ccaggacggc
     8281 ccagtcgtgt tccgatttcg cggtcagtgt ggttcgacgt gggtgaccca cgctcatcgc
     8341 cggctgcgct cgctcaacga acgggcttcg catgctcgac gcacagggat cggacagccg
     8401 tgaagagcgc cacagaaacc gcccctcacc agggcaaagc aaaaccctcg cgatgtagaa
     8461 gctacgcgag ggtttctggg ctgattgtaa tgatcagccg tgtggctccg accggcatcg
     8521 atccggtgac ctttcgattt tcagttggat gcggtggggt agcgggtgct tgctggtcgc
     8581 cgtttggtgc atgattccgc gggagtcgtc gtggttggtc gcggctggtt tggggtggtt
     8641 tcagcggagt taccggcacg gaaccggcac gaggtacctg acgggtgttc agcgtgctga
     8701 tctgcgcggc ccttcacgtg tcggtatgac tagtgggcgg agagtacttg ttcttggcgc
     8761 gcatcgttcg gaggcggtgt gcatagcgat caaggtgtgc ggaccggata tcggggctag
     8821 cgaacgactg ctgcttcaga tacacggtct tgcagagatg tagcgcgtcc tcttcaggtg
     8881 tggcgccttg tgcatacgcg tcttggcaga gtgcgtggaa gaagtgtgtg accacccagg
     8941 tgggctcagc ttcttcgatg aggctcgcgg ccttctttgc ccagcccggc gatcgctgca
     9001 ggaacagcag gagtagcgtc gcggtcaacc tctcattcgt cctactaagt tcgcccgcat
     9061 tgatggcgct ttcgagaact gacgcgagct tgggggacga cagtgtcatc tccatgccgc
     9121 caatcacggt gccggcgggg atagaacggc tcaggaagtc gacaaggtcc gcgttgtcat
     9181 cgccgtcggt tcccccttca gtttcgaggt gtcgggcaac ggcgctccta aggtctccca
     9241 aagacgagtc agagctcagc acggtgatga accgccccca cagttcgaca gtgtccacga
     9301 ggagctcctt cttgagctca aggttctcaa tttggtcgag atctcgaatc acagtggaga
     9361 ttagggtgag cacgcggtga agccgcgcgg gtggtgacat gcttgccttg acgagcccaa
     9421 acgatcccac cgagtcgctt tcggggaact caagatcgtc cccatcttca acgcgctcga
     9481 gcgcatcggt ggatcggtgc acctcttccg ggggaggtag ttcgtcaata gtgatgagag
     9541 gggtgggctc atagggtgac cctggactga ctgccaccgc tagctcctcg tctatgaggg
     9601 gccggatgcg ttggaggagg tcttcgtcag ctcgcgacag agcggacagt ttcaccgcga
     9661 ctggctgata gtagaacagg tcgttaacga ccaagtcgcg gaactccgga tcgactagcg
     9721 cgcgcttcgc cgcaaacaga gtgaagtatg catatctggc gaactcaatg gagtctccgt
     9781 ggcgcctgag gagtcttcgc tggaggaaga agtcgagtac gtgagtcgca ttagcttgcc
     9841 acccgaactt ctggatgaca tccgctatga cgcggacggc ctccggttca ctgagacttg
     9901 gtttctcgtc ccaaatgagc gattcggcaa actttccgag caccgcttcg aggtcggtct
     9961 cggtgagccc ggttgtgttc tcatgggggt tgcctatgcc caataggagc aaggtatagg
    10021 catcaagcat gctggtctga ttgcgtgtgt cgtgcgcgct ccctctgagt gcaaggtata
    10081 ggaggagtga gaccgtgagg ggtgttcggc gtagaccttc ggcctccaag accttgagcg
    10141 cctcatcagc gaggcggttg ccctgccctg gggcgaggcg ttcagctagg ttcttgacat
    10201 ccgtcttccg cattcggcca agaaagagaa tccgggggct gactccaaga gaattgagct
    10261 tagtagcgag ctcgtcttcc tctccttggt gacaaccgat gatcttgacg atggcgtcgg
    10321 aggcaacaac ctgtccgagc acggtatccg agaggcgcgg cacggacgcg cggaagtcgt
    10381 caattgcgat gatgtggtcg ggtagcgatg cgcccctgtc gtggatgagc ccacagttca
    10441 tggcggcgtt tcgcgtggcg gcccctagtg gatcgctgcg atggcggcac tccgagaatc
    10501 tgacgtagat cggcgcggcc gttccgagca attcggaaga ttgggtggct agccacttca
    10561 cggcggtggt gagtcccgaa ccttcgtctc caacaacaac gaagaagtcg tgggattcaa
    10621 tttcgcgatg gggatcaagc cgctcgacct cggcagtgtc tccgcgtcgg agccggtcgc
    10681 ggacgaatgt ggcatgcggt actggcagca gaatcggcgg aatcgtgagg tctctgcgga
    10741 cgagatcatc ggcaccggtg tcgactagta ccgattcgtt ggtcatcgaa tctcggatga
    10801 gttctctcgc ctcctgcacc acatcccggg caaggcccac ggcattagta atcagagact
    10861 cactggcggg cgcgtgcctc gccagaaagg gcgcgagatc ggcgtgctga tcaccgtagg
    10921 ccacggggac cacatgatca ttggcgtgaa gtcggcgcaa ctcgtccaac tcatcggtcc
    10981 gacacagccg ataatgagtg gtgctcgact gcgggaaaac gcggcggtgc cactccagta
    11041 gggaagcgaa gttggggtcg cctaggccgc tgccgaatcc cacgtacaca atcgacttga
    11101 gcgtcgccat cgccttttca agcgcttgca tggattcgtg agtactgagt cgtgcgtaat
    11161 ctgccgctgt aagaatcacg gagtcgggcc ggtcccagac tccgtggatg tggccgattg
    11221 cgctgctcga gcgggtgacc acatcctgaa agccgcgcga gtcggtccag tctcgcgtct
    11281 cgcggccacc tacctgttca agaagcttgt cgtagtttgt cgtgaggacg gggaacgggt
    11341 acgacaggag ggcacttgcg actgcttcgt cgctagccac gaggtccccg acatcttccc
    11401 tgagccatgc ggcgtatgca gtgtcgccta gttcgcgtat cgcgctggcc acggagctag
    11461 ctgcgttaat gaccatgtct gtcgatgggt agtcgagctg gctctgcaca atggtcagcc
    11521 attgttcgtc aaccctgttg ttcagtttgg cggcgcgctc ggctccgctc ctcactaagc
    11581 ccacccacgt ggatgtcggt gcaccgttcg tagtcgctgc ggacagcccg gtcccgacga
    11641 tgaagacgac atcggcttcg gtgatcgctt gttcgaaggc tggcacttag ttgctcctgg
    11701 gcggtatggg catgattgat cagaacgacc cgctagacag tcaacctagc ggaccgggaa
    11761 gtgacgccga ggagcgcccg gaccgcagcg gagcgaggac cggacgcacc gcaggcggcg
    11821 cagctggccg ttaggccggc cttgaacgag taaagaaagt tctcacagct tgctttccgc
    11881 ggctcgaagt gcctggccca cggctcggct cttttcggtc agacctggcg aagggtgaaa
    11941 cttcggatct gcctcggcgc acgccatggc gcgatctagc ggatctgaaa cgccgcggaa
    12001 gtcatggagt ccgagcgcgc ggatcccgac tcggagggcc tcaaggtctg gggctggcga
    12061 gaggacggcc tcaaccgccg cgacggtttt gggcgtgtac cgctatgcga acgactgcgt
    12121 aatcgcacct tgcgcggccg ccaactcgcc gaagggtggc ttcggctggt gaaacccatg
    12181 cgtgtacgcg ttgaacaggc ttcgatagtc gcgtgttgcg cggtccgcgg cctcggacgc
    12241 ggcggagatc tcgatgaatg acgcgtcgtg gtctcgtggg tcggctcccg ccgagcatcg
    12301 ctgtgtccat ggccccgact gcctctgctg cgcgctcctg gaggccctgg agcaccgagc
    12361 gaacggcgtc ggacacgact ccccatgtga cgagcgcagc ggccccgtgg ccgagttcgc
    12421 cgcccttgga gtcgttcgac ctggagttgg tcgtgacgga gcaagtcatc accactgatc
    12481 ggggaagcct ccatccggct tagcacggac tgctccttgg aatgagttca gcgcccacca
    12541 ttgatcgcct gattccatca ggaagacgta tacgctctct tcgccttcga gatcttcgaa
    12601 cacatacttc tgacccggga ccccacaggt atcgaatccg acgtggatcg tgagagcctc
    12661 gggccccgat cccttttccc actgcttgac atctgcgctc cacagatccg ctttgacccc
    12721 agagacttcg gtgctgccag tcctcgatag atcgtgaacg acggctaccg cgtccgcgga
    12781 agcaacggct gagctaggcg tggagtatga ggccatcgcg ggacagccgg aggagcctcc
    12841 cgaggagcac cccgtcaagg ctgcagacgc aatgatcaga cacgcgatgc gggagaagaa
    12901 gcgtcgagtg gaggtcatgg tgccatcttc tgataagcgg aacgcggttg aaaggcgatc
    12961 accccgactt ggttcccgcg agttttcgcc ggcgttcctc gtgcggttaa ccaccagctg
    13021 cgcgaacaac ggcagtaccg agacgatcaa ccggcgtcct cgctgacggc gggttcgaac
    13081 caccagatgg cggcgctggc ctcctgcgga tcgaaccggc atgaccggca ggcgcctccg
    13141 caccgtcgcc agtcagcacg agctggaggg gccgtgttcg tcacgtcgtg gatgaacgaa
    13201 cctcaagccg accggcacaa aacaggcaca ctcctggaga agcccaagga cgcgtctcgg
    13261 cggagacgcc ttcgagcggc tcgggcattc gatgtgaaca ggagggagag ccgggggagt
    13321 gggctggaga atccgcccct caccagagca aagcaaaacc ctcgcgatgt agaagctacg
    13381 cgagggtttc tgggctgatt gtaatgatca gccgtgtggc tccgaccggc atcgatccgg
    13441 tgacctttcg attttcagtc gaacgctcta ccaactgagc tacagagccg agcggcacat
    13501 cttgtctgcc gcttcttaga cgaagagcct cctcgaaaga aggctcaacg ctaggagcga
    13561 ccctgacggg acttgaaccc gcgacctccg ccgtgacagg gcggcacgct aaccaactgc
    13621 gctacagggc cataactttt caattgtatc ggaagagtga ccccaacggg attcgaaccc
    13681 gtgctaccgc cgtgaaaggg cggcgtccta ggccgctaaa cgatggggcc gcaggtgaac
    13741 cctgggggtt tcccttgccg acgctcaagc ataagcgaag cttcggccaa aaacgaaatc
    13801 gagggcgcgt cttcttccat cccgggcgtt tcgggaggac gatgggagtg tctcctgtgc
    13861 ccgcaggagc gcggatcacc ggtcccgaag ccgcatccgt cgcaccctct tctgttgcgg
    13921 atgtgacgaa tgttgttagt gtggcacttg tgaaaccggc ggaaggggcc gcgtgaacga
    13981 gcaggtcaag gcggatgctg ccgcatccga ggaatgcggg tgtgctccca cgcccgccga
    14041 gcggcgcgcc ttctggccct ccgcgcaggt gacccgtcgt ggcgccatca ccgtcggcgc
    14101 actcagcgcc atcgcgctca ccgcattcgg tgtcaccgcc gggacgaacg ccgcgcacgc
    14161 ggagcccggc tatcccagct gggatgacgt gcagcgcgcc aaggctaacg aggccgccaa
    14221 gggcgccgaa gtcacccgca tcgagggcct gatccagacc ctcacccagc aggccgccgc
    14281 cgcagagcag gccgctcggg tcgccggtga cgagtactac gcggcccagc aggcctactt
    14341 cgaagcggcc gaccgtgccg actcgctgca gtcgcaggcc gacgagcagg cgaagatcgc
    14401 cgacgagagc tcacgcaagg ccgctcaggt ggcgacgcag ctctaccgca ccggtggcga
    14461 ccaggccgcc ctcgagctgt tcctctccaa gtcctccaac ggtgccgacg acctgctctc
    14521 gcggttgggc agcatggaca agctgctgga gtacaaccgc accgtcgccg acacggccgc
    14581 ggccgcgcgc gactccgctc agcacctgag tgaccaggcc aagagcgcac gcgacgagcg
    14641 cgaccgactg cagaaggtcg cccaggacaa gatggtcaag gcgcaggcgg catccgacgc
    14701 cgctcaggcc gccctggacg agaagcagaa caacctgcag accctgcagg cccagctcgc
    14761 cgcgctcaag gacaccacca ctgcgacggt cgccggctat cagcagggcg aggccgttcg
    14821 caaggcccgg gaggccgccg aggccgaggc tcggcgcaag gccgccgagg aggccgccgc
    14881 caagaaccac aacaacggcg gaggcggagg cggtggtggt ggaaccggcg gctctggctc
    14941 cggtgcctgg cgtcgcccgt ccggcggccg catcacctcc tactacggcc gccgcaacgt
    15001 ccagtgcacc agcggctact gctcttcccg cgagcacggc ggcatcgaca tcgccaacgg
    15061 ctgcggtgcc ccgatctacg ccgcgcactc cggcaccgtc gacttcgccg gctacaacgg
    15121 cggctacggc aactacaccc gcatcaacca cggcggcggc gtcggcaccg gctacggcca
    15181 ccagtcgcag ttcgccgtcc gtcgcggcca gtacgtcaac gcgggccagg tcatcggcta
    15241 cgcgggcaac acgggcaact cgttcggatg ccacctgcac ttcgaggtgt acgtcaatgg
    15301 atcgcgggtc gatccgtatc cgttcatgac cgcacgcggc gctctctgag catccgctcg
    15361 ccgcacgccg gcatccgctc gacccgctga tacgacgaga cccccggcgc gaaccggggg
    15421 tctcgtcgct tcgacaggct cagcgaccca tcggatgaag gtcagtggcc ctcggcgccg
    15481 aagcgcgcga tcgcctcgtc gaggatgcgc tgcgcctcag cagcgtcgcc ccactcgtcg
    15541 accttgaccc acttgttggg ctcgaggtcc ttgtagtgct cgaagaagtg cgcgatctcc
    15601 ttcttggtgt actccgccag gtcgtcgacg tcctggatgt gcgcccagcg cgggtccttc
    15661 gacagcacgg ccacgagctt gtcgtcccca ccggcctcgt cgctcatctt cagcaccgcg
    15721 acggggcgca cgttcacgac gacgcccggg tagatggcgt ggtcgagcag cacgagcacg
    15781 tccagcgggt cgccgtcctc gccgagggtg ttgtcgaagt agccgtagtc ggccgggtat
    15841 ccgaacgtcg tgtacagcac gcggtcgagg tgcacgcgac ccgtctcgtg gtcgacctcg
    15901 tacttcacgc ggctgccgcg cgggatctcg atgacggcgt cgtgtgcgcc catgtgcttc
    15961 tccttggttc ggatgccggg atcgagcgga tgccggcggc ggaagttcgc ggggagccca
    16021 gcggccgccc cgcacaagga tactcgcgcg ccgtgcggaa ctttccgggg tcaggccttc
    16081 ggcacccaga cgcagacgac ggagtcgtcg gtctgctggt cggggaggaa gcccgtcaga
    16141 tgccacccgt ccggttcgag gaacgggtag tgctggatgg gcgtgtagag cttctcaccc
    16201 gtcgcggagg tctgcggcgc gtcatccgag gggcccaccc tgccctgcgc ctgcgcgaag
    16261 tcgccctcgg tgtacacctt caccgggcag tagccgccga agccctgccc gtcgagggtg
    16321 accggccagg agccggcacc ggtgccgtcg cccgcggcac cgacgtcgcg tgtgcgctcc
    16381 atggtgccgg actgcgcgca cggtcccgtg gcctcgaacg agttgcgcat cacctcgtgc
    16441 acccggaacc cgcccgcgat cgcctcgacc ccggtgaccc ggtcggcgca gcgcgcgccg
    16501 gacgcgatcg ggaacgggat ctgctgcggc tggtccggag catccgcctg cgccagccag
    16561 atgaaccagt tgttcatgat cccgttgccg gactcgaact gcagcggcac ggcggcgtcg
    16621 atcaggccgt ccccgttcgc atcggcgaac gtcggctcgc cgagctggaa cagcgcggtc
    16681 tgcttgtagt acggatcctc cacctcggcc ctgccgccgc tgagcttcac cgggtactgc
    16741 acgcccagcg tgtcccaggt gaaggtcatc gatccgaagt ccacagcgcg gatggcatcg
    16801 gcggcgggcg tgggggagga gctcgcgtgc gggctcggct tcggcgacgc cggtggcggc
    16861 gtgggcgtgc accccgtgaa cagcgcggcg acgagcagcg cgatgacgaa gactgaatcc
    16921 ccccggctca tgctggcagg ctagcgaagg tcgcggatcg cgtgcgggcg gcgccgccgg
    16981 atcgtgccgg atctgtgacc cggccgaccg gccccgtacg gtggaggaat gccatcgctc
    17041 gatcccgccg tcgccgaagt ccgccgtgcc gtgcgcgcga ccctcgccgc tctggaggcg
    17101 ggtcccgtgg tcgtcgcgct ctccggcgac gccgactcgc ttgccctcac agcggccacc
    17161 gtcttcgagg cgcgggcgct cggcctcccg gtcacggccg cgatcgtcga tcacggtctg
    17221 caacccggat cggatgccgt cgcccagcgc gcggccgacc aggcgcggga gctcggagcg
    17281 gatgcccggg tgctgagcgt gcacgtggat cagcagcatc cggacggcct cgaggccgcc
    17341 gcacgtgccg cccgctacaa cgtgctgcgg cagtgcgctg cggaactcgg cgcatcggcc
    17401 gtgatgctcg gccacaccct cgacgatcag gccgagaccg tgctgctggg actcgcccgc
    17461 ggctccgggg cggccagtct gcagggcatg gcacagctgc gggaggacgc c
//
LOCUS       DATMSX010000476         8808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_92983,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000476 DATMSX010000000
VERSION     DATMSX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92983"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..798
                     /locus_tag="VN041_15200"
     CDS             259..798
                     /locus_tag="VN041_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU30427.1"
                     /translation="MAPTDHGRPTPSRFTTERLLLRARTMDDFEACLEMDRDPEVTAY
                     VDGPWNDPDEHRAFLTQRILATYPPGHGYWAITTHDSPEDLLGWVLVLPTDEAGVAEI
                     GWRLVRRAWGRGYATEAARRILDHVWADTAVTRVTADIHPDNRASIAVATKLGLRLEG
                     ARPDGALRHSMAAPLARRR"
     gene            824..2278
                     /locus_tag="VN041_15205"
     CDS             824..2278
                     /locus_tag="VN041_15205"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWU30428.1"
                     /translation="MLNMAEGLSRLGALAAHPVVRTLGEAFQTAGFELAVVGGPVRDA
                     LLGRETHDLDFTTNAQPDEILAIVKPLASAHWDIGRAFGTIGARVQGEQVEITTYRAD
                     SYDGLTRKPVVEFGDSIDGDLLRRDFTVNAMALSVATPAQRQTGPGPKLVDPTGGVED
                     LVAGILRTPIDPAVSFGDDPLRMLRAARFSSQLEFAIDDATFAAIEQLRQTLSIVSPE
                     RVQSELIRLMSTSDPVRGLRVLVDSGLIDQFLPEVSALRLEVDEHHHHKDVYEHSLTV
                     VRQAIELERTRNPGADPDVPLRLAALLHDIGKPRTRKLEPGGGVTFHHHDVVGSRMAR
                     KRLQALRFDGETTDAVAKLIELHLRFFGYAEGAWTDSAVRRYVRDAGDLIERLHILVR
                     ADVTTRNKRKAARLATAYDDIERRIAELREQEELDSIRPELDGNDIQRILDIPPGREV
                     GEAYRFLLDVRLDEGVIGADAAAQRLREWWAARE"
     gene            2423..4051
                     /locus_tag="VN041_15210"
     CDS             2423..4051
                     /locus_tag="VN041_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30429.1"
                     /translation="MKISRGRLGLAIGALGVAAVVLSGCATSTADKGGDSGSAKGGDL
                     IVGTTDKITSLDPAGSYDNGSFAVMNQVYPFLLNSKYGDAKVSPDIAESAEFTSPTEY
                     TVKLKKGLTFANGHKLTSSDVKFTFDRMVKINDPNGPASLLGNLASVAAPDDTTVVFT
                     LKNGNDQIWPLILSSPAAPIIDEEVFSPDAVTPDADIVKGNPFAGQYTIDSYKVNELI
                     SYKPNKDYQGNLGAAENSGIQAKYYADASNLKLAIGNGDIDVAFRSLSATDVESLKDN
                     SKVKIVDGPGGEIRYIVFNFDTMPFGAKTPEADPAKALAVRQAMADLIDRDAVAKDVY
                     KDTYSPLYSFVADGMPGATTVLKDMYGDGKGGPDQAKAEKALKDAGVTTPVTLNLQYN
                     GDHYGPSSGDEYAAYKTQLEKGGVFKVNLQQTEWVQYSKDRTADVYPMYQLGWFPDYS
                     DPDNYLSPFFVKDNFLANHYDNPAVNDAIVKQQTETDSAKREEQIGQIQEMVAKDLST
                     LPILQGKQVAVVGKDVQGTTLDASFKFRYAPLKK"
     gene            4137..5213
                     /locus_tag="VN041_15215"
     CDS             4137..5213
                     /locus_tag="VN041_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30430.1"
                     /translation="MAVDGAVLAPVQPRSRSKGGGLWRYILIRLLLIIPTVFILVTTV
                     FFLMRITGDPITAALGGRLPPEQLTQRIHEAGYDRPLVVQYLEYLGNVFRGDFGTTIT
                     DRRPVTEILLQYGSATLELAIYALVVALIISIPLGLIAAYRRDRWQDASLRVMAILAY
                     ATPIFFAGLLLKLIFSVWLDWLPVSGRGSTRTELLMGGIDTPTGIYLLDAIRLGNGAA
                     IADVLQHAILPAIALGLLTAGVFLRLIRTNVIGTLGQQYVTSGRSRGVSEYRLVTKHA
                     YRPALIPIITVMGMQIAMLLAGAVLTETTFEWKGIGFMLADYLKSRDFVAVQGIVVMI
                     AVVVAVTNFLVDIIAAFIDPRVRY"
     gene            5242..6249
                     /locus_tag="VN041_15220"
     CDS             5242..6249
                     /locus_tag="VN041_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017791946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30431.1"
                     /translation="MPTPQTAVLAAAPVRKKRWTERVPVIKELRQSVGLQRGMLITGL
                     VITLLFVVIALLAPLLAPYTWAQQQTADGHSFGTQQAPNALNLLGTTVSGFDVLSRVL
                     WGAQTALLVIIAAIACSIFIGIALGLISGYFGGWLDRLLVVVADAIYAFPSLLLAIVM
                     SIVISRGQSTLGGGIMATAIAITVVFVPQYFRVIRAEVVRVRAEPFVDSAKVIGVPTT
                     RILLRHVLRNSTRSLPVIITLNASEALLTLAGLGFLGFGIEATAAAEWGYDLNRAVGD
                     VTSGIWWTSVFPGVAIVLVVLGVTLVGESLNDLADPRLRLRRRAGGKKKVGAANEGAK
                     A"
     gene            6246..7988
                     /locus_tag="VN041_15225"
     CDS             6246..7988
                     /locus_tag="VN041_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30432.1"
                     /translation="MSAAEKNRMPADLAPAVAEIHDLKVMFATDDEPVEAVKGISLSA
                     YAGEVLAIVGESGSGKTVTANTLLGLLPETATTSGAVIVRGRAGDETDIVHADHARMR
                     RMRGRDASMVFQEPSTALNPVYTVGWQIAEGIRAHATVSKKDARARAIEIMQRVGIPD
                     AANRVDDYPHQFSGGQKQRIVIAMALVLDAGLIIADEPTTALDVTVQAEILDLLRTAR
                     DEFGATIVLITHNMGVVADLADRVIVMYQGDIVEEAPVRELFANPREEYTKKLLAAVP
                     HVGQGKSAAGSAARVTEPDAAPHTAPPVPEGSLIVASAAEIGYPGRFGRNAVVAVKGV
                     DFHVGPGEVVGLVGESGSGKTTIGRAVVGLTTVLGGSLTVLGQEMRGVKPRTLTPLRP
                     KVGFVFQDPATSFNPLLSIAECIAEPLIVHGRASSLQAARPRVNELLDAVRLPTSFGD
                     RYPHELSGGQRQRASLARALALDPELLIADEPTSALDVSVQATVLELFVHLQAELGFA
                     SLFISHDLAVIDAVSDRIIVLQQGRIQEQGTTAQVLGDPRDPYTRELLAALPVPDPVA
                     QAERRAQWEAVRRH"
     gene            8192..>8808
                     /locus_tag="VN041_15230"
     CDS             8192..>8808
                     /locus_tag="VN041_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU30433.1"
                     /translation="MPSGWSSSRDPSPEASRLLIERAHEELVAGNLDDRRLQQVRPIV
                     RDSWQRAWRDRVGAEGLPRLDFTREALETYRTAHPLAGAMDLIRTLLLPGAPEDSGVI
                     VAVGDHAGRLLWVEGDLQQRMLSGEMGFVEGANWSEGVVGTAAPGTALALGESVQIHH
                     AEHYNRLVQPWSCTAAPVFDPETRRILGVLDVTGGSEAATPQARML"
BASE COUNT         1418 a         3139 c         2926 g         1325 t
ORIGIN      
        1 cccgcccggg cgatctggcg accaccggcg gctccgccct gtggccgatc gggctcgcgg
       61 ccggtgcact gctgacagtg accgtcggcg gcatcctcgt ccgccggggt cgccgggacg
      121 cctgaccctc gcagttctga aggatggatg ccgttctgaa ggatgccgtg acccggatcg
      181 tccttcagaa cgtgcatcct ccttcagacc cgggacaggt cctgccgggc ggtgggcaac
      241 gccgtagaat ctgcggtcat ggctccgacc gatcacgggc gcccgacgcc gagcaggttc
      301 accaccgagc gcctgctcct gcgcgcgcgg acgatggacg acttcgaggc ctgtctggag
      361 atggaccgcg accccgaggt gaccgcgtat gtggatggcc cctggaacga tcctgacgag
      421 caccgcgcct tcctcacgca gcggatcctc gcgacctatc cgccgggcca cgggtactgg
      481 gcgataacca ctcacgattc gcccgaggat ctcctcggct gggttctggt cctgccgacc
      541 gacgaggcgg gcgtggcgga gatcggctgg cgactggtcc gcagggcgtg gggccgcgga
      601 tacgccaccg aggccgcccg gcgcattctc gaccacgtct gggcggacac cgcggtgacc
      661 agggtcacgg cggacatcca ccccgacaat cgcgcttcga tcgcggtggc caccaaactc
      721 ggcctcaggc tcgagggcgc ccgtcccgat ggcgcgctgc gccattcgat ggccgcgccc
      781 ctggcgagac gccgctgact cgacccccgg taatctggaa cccatgctca acatggccga
      841 gggcctgtcc cgactcggcg cgctcgccgc gcatccggtc gtccgcaccc tcggcgaggc
      901 cttccagacg gcgggcttcg agcttgcggt ggtcggcggc ccggtccgcg acgccctgct
      961 cggccgcgag acgcacgacc tcgacttcac cacgaacgcg cagcccgacg agatcctcgc
     1021 gatcgtcaaa ccactggcat ccgctcattg ggacatcggc cgcgccttcg gcacgatcgg
     1081 cgcgcgcgtg cagggcgagc aggtggagat caccacctac cgtgccgaca gctacgacgg
     1141 tctcacccgc aagccggtcg tcgagttcgg cgacagcatc gacggcgacc tgctgcgtcg
     1201 cgacttcacg gtcaacgcca tggcgctcag cgttgccaca ccagcgcagc ggcagacggg
     1261 gccgggtccg aagctcgtgg acccgaccgg tggcgtggag gacctcgtcg ccggcatcct
     1321 gcgcaccccc atcgacccgg ccgtcagctt cggcgacgat ccgctgcgga tgctgcgcgc
     1381 ggcgcgcttc agctcccagc tggagttcgc gatcgacgac gccaccttcg ccgccatcga
     1441 gcaactgcgc cagacgctca gcatcgtcag ccccgagcgc gtgcagagcg agctgattcg
     1501 gctgatgagc acctccgacc cggtgcgcgg actgcgcgtg ctggtcgaca gcgggctgat
     1561 cgatcagttc cttcctgagg tcagcgcgct gcgcctcgag gtcgatgagc accaccacca
     1621 caaggacgtc tacgagcact cgctcacggt cgtgcggcag gccatcgagc tcgagaggac
     1681 ccgcaacccc ggcgccgatc ccgacgtgcc gctgcggctc gccgcgctgc tgcacgacat
     1741 cggcaagccg cgcacccgca agctcgagcc gggtggggga gtgaccttcc accatcacga
     1801 cgtggtgggc tcgcgcatgg cgcgcaagcg actgcaggcg ctgcgcttcg acggcgagac
     1861 gacggatgcc gtggcgaagc tcatcgagct gcacctgcgc ttcttcgggt acgccgaggg
     1921 cgcctggaca gacagcgcgg tgcgccgcta cgtccgcgac gccggagacc tgatcgagcg
     1981 cctgcacatc ctggtgcgcg ccgacgtcac cacccgcaac aagcgcaagg cggcgcgcct
     2041 cgccaccgcc tacgacgaca tcgagcgccg catcgccgag ctccgcgagc aggaggagct
     2101 ggacagcatc cgcccggagc tcgacggcaa cgacatccag cgcatcctcg acatcccgcc
     2161 gggtcgcgag gtcggggagg cgtaccgttt cctgctcgac gtgcggctgg acgagggcgt
     2221 catcggggcg gatgccgccg cgcagcgcct gcgcgaatgg tgggccgcgc gcgaatgatt
     2281 cggccgtgta aacgccgggt gtcggtctaa tcacgatcct gaccgctcgg ttgggtatgt
     2341 acatgcgggt gattagattg acaatcatga gccgcgccga cgacgtgcgc ggaaacccga
     2401 catcacaggg caggtcatat caatgaagat ctccagaggc cgactcggcc ttgccatcgg
     2461 cgcgctcggc gtcgcggcag tcgttctctc cggttgcgct acgagcacgg ccgacaaggg
     2521 cggcgacagc ggcagtgcga agggcggtga cctcatcgtc ggaaccaccg acaagatcac
     2581 ctcgctcgac ccggccggtt cgtacgacaa cggctccttc gcggtgatga accaggtgta
     2641 cccgttcctg ctgaacagca agtacggcga cgccaaggtc tcgcccgaca tcgcggagtc
     2701 ggcggagttc acctctccga ccgagtacac cgtcaagctc aagaagggcc tgaccttcgc
     2761 gaacggccac aagctcacct cgagcgacgt gaagttcacc ttcgaccgca tggtcaagat
     2821 caacgacccc aacggcccgg cctcgctgct cggcaacctg gcctcggtcg ccgcacccga
     2881 cgacaccacc gtggtcttca cgctgaagaa cggcaacgac cagatctggc cgctgatcct
     2941 ctccagcccc gctgctccga tcatcgacga agaggtcttc tcgcccgacg ccgtcacccc
     3001 cgacgccgac atcgtcaagg gcaacccctt cgccggtcag tacacgatcg actcgtacaa
     3061 ggtcaacgag ctcatctcgt acaagccgaa caaggactac cagggcaacc tcggcgcagc
     3121 cgagaactcg ggcatccagg cgaagtacta cgccgacgcc tccaacctga agctcgcgat
     3181 cggcaacggc gacatcgacg tggccttccg cagcctgtcg gccaccgacg tcgagagcct
     3241 gaaggacaac tcgaaggtga agatcgtcga cggccccggc ggcgagatcc gctacatcgt
     3301 cttcaacttc gacacgatgc cgttcggcgc gaagacccct gaggccgacc cggccaaggc
     3361 gctcgccgtg cgtcaggcga tggccgacct gatcgaccgc gacgccgtgg cgaaggacgt
     3421 ctacaaggac acctacagcc cgctgtactc gttcgtcgca gacggcatgc ccggcgccac
     3481 cacggtgctg aaggacatgt acggcgacgg caagggcggc cccgaccagg ccaaggccga
     3541 gaaggcgctg aaggatgccg gtgtcaccac gccggtcacc ctgaacctgc agtacaacgg
     3601 cgaccactat ggcccctcct cgggtgacga gtacgccgcg tacaagacgc agctcgagaa
     3661 gggcggcgtg ttcaaggtca acctgcagca gaccgagtgg gtgcagtact ccaaggaccg
     3721 cacggccgat gtctacccga tgtaccagct cggctggttc ccggactact ccgacccgga
     3781 caactacctg tcgccgttct tcgtgaagga caacttcctc gcgaaccact acgacaaccc
     3841 ggcggtcaac gacgccatcg tcaagcagca gaccgagacc gactccgcca agcgcgagga
     3901 gcagatcggc cagatccagg agatggtcgc gaaggacctc tccaccctgc cgatcctgca
     3961 gggcaagcag gtcgcggtcg tgggcaagga cgttcagggc accacgctcg acgcctcgtt
     4021 caagttccgc tacgcgccgc tgaagaagta acgcgttccg cattcggggg tgctgcccag
     4081 cgcagcaccc ccgaatgcgc tcgccccgaa gaacgcaaca gttaggtagt acccccatgg
     4141 ccgtcgacgg cgccgtcctc gcccctgtcc agccccgaag ccgctcaaaa ggcggcgggc
     4201 tgtggcgtta catcctgatc agactcctgc tgatcatccc cacggtcttc atcctggtga
     4261 ccaccgtctt cttcctgatg cggatcaccg gagatccgat cacagccgcg ctcggcggcc
     4321 ggctcccacc ggagcagctg acgcagcgca tccacgaggc cggctacgac cggcccctgg
     4381 tcgtgcagta tctcgagtac ctcggcaacg tgttccgcgg tgacttcggc accacgatca
     4441 ccgatcgtcg ccctgtcacc gagatcctgc tgcagtacgg ctcggccact ctcgagctcg
     4501 cgatctacgc gctggtcgtg gcgctgatca tcagcatccc gctcggcctg atcgccgcgt
     4561 accgccgcga ccgctggcag gacgcctcgc tgcgcgtcat ggcgatcctc gcctacgcga
     4621 ccccgatctt cttcgcgggt ctgctgctca agctcatctt ctcggtgtgg ctggactggc
     4681 tcccggtctc aggacggggg agcacccgca ccgagctgct gatgggcggc atcgacaccc
     4741 ccaccggcat ctacctgctc gacgccatcc gcctcggcaa cggcgccgcc atcgccgacg
     4801 tgctgcagca cgccatcctc cccgccatcg cgctcgggct gctcaccgcg ggcgtcttcc
     4861 tgcgcctgat ccgcacgaac gtgatcggca cgctcggtca gcagtacgtc acctcgggcc
     4921 gctcgcgcgg cgtgagcgag taccgtctgg tcaccaagca cgcctaccgt cctgcgctca
     4981 tcccgatcat cacggtcatg ggcatgcaga tcgccatgct gctggcggga gcggtgctga
     5041 cggagaccac cttcgaatgg aagggcatcg gcttcatgct ggccgactac ctgaagtccc
     5101 gtgacttcgt ggccgtgcag ggcatcgtcg tgatgatcgc cgtggtggtc gccgtcacga
     5161 acttcctcgt cgacatcatc gcggcgttca tcgacccgag ggtgaggtac tgagatgtcg
     5221 atccccgact cctacgacga gatgcccacg cctcagaccg cggtgctggc cgccgccccg
     5281 gtgcggaaga agcgctggac cgagagggtc ccggtcatca aggagctgcg ccagagcgtc
     5341 ggcctgcagc gcgggatgct gatcacgggc ctcgtgatca ccctgctgtt cgtcgtgatc
     5401 gccctgctcg ccccgctgct cgccccgtac acctgggcgc agcagcagac cgccgacgga
     5461 cactccttcg gcacgcagca ggctccgaac gcgctgaacc tgctcggcac caccgtctcc
     5521 ggcttcgacg tgctctcccg cgtgctgtgg ggagcgcaga cagcgctgct ggtgatcatc
     5581 gccgccatcg cctgctcgat cttcatcggc atcgcgctcg gcctgatctc cggctacttc
     5641 ggcggatggc tggaccggct gctggtggtc gtcgccgacg cgatctacgc gttcccttcg
     5701 ctgctgctgg cgatcgtgat gtcgatcgtg atcagccgcg ggcagtcgac cctcggcggc
     5761 ggcatcatgg ccaccgcgat cgcgatcacc gtggtgttcg tgccgcagta cttccgcgtc
     5821 atccgagccg aggtcgtgcg tgtgcgggcc gagccgttcg tggactccgc gaaggtgatc
     5881 ggcgttccga ccacccgcat cctgctgcgc cacgtgctgc gcaactcgac ccggtcgctg
     5941 ccggtgatca tcacgctcaa cgcctccgag gcgctgctga ccctcgcagg cctgggcttc
     6001 ctgggcttcg gcatcgaggc caccgccgca gccgagtggg gttacgacct gaaccgcgcc
     6061 gtgggagacg tgaccagcgg catctggtgg accagtgtgt tccccggcgt cgcgatcgtg
     6121 ctggtcgtgc tgggcgtcac tctggtcggc gagagcctga acgacctggc cgacccgcga
     6181 ctgcggctgc gccgccgcgc gggcggcaag aagaaggtcg gggctgcgaa tgagggagcg
     6241 aaggcatgag cgccgctgag aagaaccgga tgccggccga cctggcgccc gcggtcgcgg
     6301 agatccacga cctgaaggtc atgttcgcca ccgacgacga gccggtcgag gcagtcaagg
     6361 gcatctcgct gagcgcctac gccggcgagg tgctcgcgat cgtcggcgag tccgggtcgg
     6421 gcaagaccgt cacggccaac accctgctgg gcctgctgcc cgagaccgcc accacctcgg
     6481 gggcggtcat cgtgcggggt cgcgcggggg acgagaccga catcgtgcac gccgaccacg
     6541 cccgcatgcg ccgcatgcgc gggcgcgacg cgtcgatggt gttccaggag ccgtccacgg
     6601 cgctgaaccc ggtgtacacc gtgggctggc agatcgccga gggcatccgc gcgcacgcga
     6661 cggtctccaa gaaggatgcc agggcgcgcg cgatcgagat catgcagcgc gtcggcatcc
     6721 cggacgccgc caaccgcgtg gacgactacc cgcaccagtt ctcgggcggg cagaagcagc
     6781 gcatcgtcat cgcgatggcg ctggtgctgg atgccgggct gatcatcgcc gatgagccga
     6841 cgacggcgct cgacgtgacc gtgcaggccg agatcctcga cctgctccgc acggcgcgcg
     6901 acgagttcgg cgcgaccatc gtgctgatca cgcacaacat gggcgtggtc gccgatctcg
     6961 ccgaccgcgt gatcgtgatg taccagggcg acatcgtgga ggaggcgccg gtccgcgagc
     7021 tgttcgcgaa cccccgcgag gagtacacga agaagctgct cgctgcggtc ccgcacgtgg
     7081 gccagggcaa gtcggccgcc ggctccgccg cgcgggtgac ggaaccggat gccgcgccgc
     7141 acaccgcccc gccggtgccg gagggttcgc tgatcgtggc atccgctgct gagatcggct
     7201 acccgggccg cttcggccgc aacgcggtgg tggcggtgaa gggcgtcgac ttccacgtcg
     7261 gccccggcga ggtcgtcggc ctggtcggag agtccggctc cggcaagacc accatcggcc
     7321 gtgccgtggt gggtctcacg accgtgctgg gcggctcgct gaccgtgctc gggcaggaaa
     7381 tgcgcggcgt gaagccgcgc accctcaccc cgctgcgccc gaaggtcggg ttcgtgttcc
     7441 aggacccggc gaccagcttc aacccgctgc tgtcgatcgc cgagtgcatc gccgagccgc
     7501 tgatcgtgca cggccgggcg tcgagcctgc aggcggcgcg cccgcgcgtg aacgagctgc
     7561 tcgacgcggt gcggctgccg acgagcttcg gcgaccgcta tccgcacgag ctctcgggcg
     7621 gtcagcgtca gcgggcgtcg ctggcgcgcg cgctggcact cgacccggag ctgctgatcg
     7681 ccgatgagcc gaccagcgcg ctggacgtct cggtgcaggc gacggtgctg gagctgttcg
     7741 ttcacctgca ggccgagctg gggttcgcgt cgctgttcat cagccacgac ctggcggtga
     7801 tcgatgccgt gtccgacagg atcatcgtgc tgcagcaggg ccgcatccag gagcagggca
     7861 ccaccgccca ggtcctcggc gacccgcgcg acccctacac ccgcgagctg ctcgccgccc
     7921 tgcccgtgcc cgacccggtc gcccaggccg agcgtcgtgc ccagtgggag gcggtgcggc
     7981 ggcactagcc ggcgccttcg ctcgggagga gaactcacgg atgggaggag ggttccggag
     8041 gatttctcct cccatccatg agaagtcctc ccgagcagga gcaggagcag gagcaggcac
     8101 ggacgcgggc tcggccgcgc gccacccgtt ccccgtgacc gggcatccga cctacactgg
     8161 gcacaccccg tcagcgccga tcggaggctc cgtgccgtct ggatggtcat cgtcgcgcga
     8221 cccctcgccg gaggcgtcgc ggctgctcat cgagcgcgcg cacgaggagc tcgtcgccgg
     8281 caacctcgac gatcggcgcc tgcagcaggt gcgtccgatc gtgcgggact cgtggcagcg
     8341 cgcctggcgg gaccgggtcg gtgccgaagg gctgccacgg ctcgacttca cacgggaggc
     8401 gctggagacc taccgcaccg cgcaccccct ggcgggggcg atggatctga tccgcacgct
     8461 gctgctgccc ggcgcgccgg aggactccgg cgtgatcgtc gccgtcggcg accacgcggg
     8521 gcggctgctg tgggtggagg gcgatctgca gcagcggatg ctctcgggcg agatgggctt
     8581 cgtcgagggg gcgaactggt cggagggtgt cgtgggcaca gccgcaccgg gcaccgcgct
     8641 cgcgctgggc gagtcggtgc agatccacca cgccgagcac tacaaccggc tcgtgcagcc
     8701 gtggtcctgc accgccgcac ccgtgttcga ccccgagacg cgccgcatcc tgggcgtgct
     8761 cgacgtcacc ggcgggagcg aggcggcgac gccgcaggcg cggatgct
//
LOCUS       DATMSX010000477         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_73865,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000477 DATMSX010000000
VERSION     DATMSX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73865"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VN041_15235"
     CDS             complement(<1..432)
                     /locus_tag="VN041_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30434.1"
                     /translation="MTEDADPVRTRSRILDAAEALFARHGFDGTSTARLADAAGVPKG
                     LLFYYFRTKPAILSALLEERLDPAALDPAVLAVPGDPVQTLVNVGERVLGDRAASGVL
                     REVIWHEAHTRPDVRAVLTRYRHALHETIEHALRASLTAAVD"
     gene            complement(512..886)
                     /locus_tag="VN041_15240"
     CDS             complement(512..886)
                     /locus_tag="VN041_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HWU30435.1"
                     /translation="MKRWTRWQDWVAVAAGLYAVLATMWTTPRTGASVSMMVVLGILM
                     IAAAVWSLAAPGLVSMEWILAAIGALLFLSPWFGSFAGSAGPAWTAWICGGVGVVAGL
                     WALAPAEKAHHATPGGHRAAHA"
     gene            1014..>1708
                     /gene="dinB"
                     /locus_tag="VN041_15245"
     CDS             1014..>1708
                     /gene="dinB"
                     /locus_tag="VN041_15245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWU30436.1"
                     /translation="MGRGDGSGRLVSPEGAEDAGTGILHVDMDAFYASVEILYDPSLR
                     GLPLIIGAPDGRSVVSSASYEARRFGVRSAMPVSQALRLCPQARVVPPDFTKYRAVSS
                     AVMDVFHSVTPLVEQLSIDEAFLDVRGVRRLWGSPGRIAVLLRRRVLEEVGITCSVGV
                     AATKHVAKMASTISKPDGMLIVPAARTQEFLDPRPVGAMWGVGPKAAEALERRGIRLI
                     RDVRETPLDALSRA"
BASE COUNT          243 a          589 c          617 g          259 t
ORIGIN      
        1 gtccaccgcg gcggtcaggc tggctcgcag ggcgtgctcg atcgtctcgt gcagcgcatg
       61 gcggtagcgg gtgagcacgg cgcgcacatc cgggcgggtg tgcgcctcgt gccagatcac
      121 ctcccgcagc acgccggacg cggcgcggtc gccgaggaca cgttcgccca cgttgaccag
      181 cgtctgcacc ggatcgccag gcactgccag tactgcaggg tcgagcgccg cggggtcgag
      241 ccgctcctcg agcagtgcgc tgagaatcgc gggcttggtg cggaagtagt agaacagcag
      301 gcccttgggt actcccgcgg catccgcgag gcgcgccgtg gaggtcccgt cgaagccgtg
      361 gcgggcgaag agcgcctccg ccgcatccag gatgcggctg cgggtgcgca ccggatcggc
      421 gtcctcggtc atctcgccct ccccggcgtc atcgtgtcgt gatgcggggc ggcggtcgcc
      481 cgtcgccccg atcgtcgtcg cgcatcgcag gtcaggcgtg cgctgcccga tgtccgcccg
      541 gagtcgcgtg gtgcgccttc tccgcgggtg cgagcgccca gaggcctgcg accaccccga
      601 caccaccgca gatccacgcg gtccacgcgg gaccggcgct tccggcgaag gagccgaacc
      661 acggcgagag gaacagcagc gcaccgatcg cggcgaggat ccactccatc gacacgagtc
      721 ccggcgccgc caggctccac accgcggccg cgatcatcag gatgcccagc acgaccatca
      781 tggagaccga cgcgcctgtg cgcggagtcg tccacatggt cgccaggacg gcgtacagtc
      841 cggcggcgac ggccacccag tcctgccagc gtgtccacct cttcattgaa ctcatcctcc
      901 ttgcagaaag aatggctccc gaatcggggg tcgatgccca gtgtacgccg ggttttgacc
      961 gaacggtcaa gattcgtgca tccggtgtcg gcggcctccg ggacaatgga gggatgggac
     1021 gtggagacgg atcgggacgg ctcgtctcgc cggagggggc ggaggatgcc gggaccggca
     1081 tcctgcacgt cgacatggac gcgttctacg cctcggtcga gatcctgtac gacccgtcgc
     1141 tgcgcggact gccactgatc atcggcgcgc cggacggtcg ctccgtggtc tccagcgcgt
     1201 cctatgaggc gcgacgcttc ggtgtccgca gtgcgatgcc ggtgtcgcag gcgctgcgtc
     1261 tgtgcccgca ggcgcgcgtg gtcccgcccg acttcacgaa gtaccgcgcc gtctcctccg
     1321 cggtgatgga cgtgttccac agtgtcaccc cgctggtgga gcagctctcc atcgacgagg
     1381 cgttcctcga cgtgcgcggc gtgcggcggc tgtgggggag tcctgggcgg atcgcggtgc
     1441 tgctgcgccg gcgcgtgctg gaggaggtgg gcatcacctg cagcgtgggc gtcgctgcga
     1501 cgaagcacgt cgcgaagatg gcatccacca tctccaagcc ggacgggatg ctcatcgtgc
     1561 cggccgcgcg cacccaggag ttcctcgatc cgcggccggt cggcgcgatg tggggggtgg
     1621 gtcccaaggc cgccgaagcg ctggagcgac gcggcatccg cctcatccgc gatgtgcggg
     1681 agacgccgct cgacgcgctg tcccgggc
//
LOCUS       DATMSX010000478         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_187360,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000478 DATMSX010000000
VERSION     DATMSX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187360"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VN041_15250"
     CDS             complement(<1..781)
                     /locus_tag="VN041_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWU30437.1"
                     /translation="MTTTETPLVRVLEADGSFAPSPASEQYLPVIDALTDAELEQFYR
                     DMVIIRAIDTQATNLQRQGQLALWPPSRGQEAAQVGSAHAARRQDTIFPSYREHAVTR
                     IRGVDPVDILKVMRGISHGGWDPTDSKNGNTRIYTLVLGSQTLHAAGYGMGLAFDGKT
                     GSGDPERDEAVVVYYGDGASSQGDVHEAMVFAASYDAPVLFFLQNNHWAISVPVETQS
                     KVPLVGRGAGYGIPSVRVDGNDVLASYAVSRALLDESRAGLG"
     gene            complement(869..2047)
                     /locus_tag="VN041_15255"
     CDS             complement(869..2047)
                     /locus_tag="VN041_15255"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HWU30438.1"
                     /translation="MTYDFETVVDRTGTGASKWQHMLTANPDVPAGISPFSVADIDLK
                     LAPEILDGLRDHLGDVVLGYTVATDDYWDAVTGWFQRRHDWTVDREQIVLAPGVVPAF
                     NTAVRAVTAPGDGVIVQTPAYYPFFGAIEGNDRVVVRNPLVLQDGRWRLDLDDLAAKA
                     ADPRTTALLFCSPHNPTGRVWEREELEAVWRIARDNDLTVISDEIHFDLLMPGIRHTV
                     FSTLNEDAAARTIVCTAPSKTFNLAGMATSNIVIPNPELREKYAAEQSRTGFFTLNAL
                     GFQACRLAYTKAEGWLDGLIDLVHGNHELVRSFLAERMPEVTVYDLEGTYLQWMDFRG
                     LDCTHEELKRIHEQEALVFFDEGAMFGPEGEGFERMNLAAPRHAIAPALERLAQAHGR
                     "
     gene            complement(2044..>2293)
                     /locus_tag="VN041_15260"
     CDS             complement(2044..>2293)
                     /locus_tag="VN041_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU30439.1"
                     /translation="SVIVERRTRLIAALRAQGWDVPDSQANFVWLPSGERTDEVAAAF
                     DAADLIVRPFSGDGIRISVGEEESLDKVLAVAAAQVRA"
BASE COUNT          337 a          764 c          831 g          361 t
ORIGIN      
        1 ggccgagccc ggcgcgcgac tcgtcgagca gggcgcggga gacggcgtag ctggcgagca
       61 cgtcgttgcc gtcgacgcgg accgacggga tgccgtagcc ggcaccgcgg ccgacgaggg
      121 gcaccttgga ctgggtctcg acgggcaccg agatcgccca gtggttgttc tgcaggaaga
      181 acagcacggg cgcgtcgtaa ctggccgcga acaccatcgc ctcgtgcacg tcaccctggc
      241 tggaggcgcc gtcgccgtag tagaccacga cggcctcgtc gcgctccggg tcgccggagc
      301 ccgtcttgcc gtcgaaggcg aggcccatgc cgtagcccgc ggcgtgcagc gtctgcgagc
      361 cgagcaccag cgtgtagatg cgggtgttgc cgttcttcga gtccgtcggg tcccagccgc
      421 cgtgagagat cccgcgcatc accttgagga tgtcgaccgg gtcgacgccg cggatgcggg
      481 tgaccgcgtg ctcccggtag gaggggaaga tcgtgtcctg ccgacgcgcg gcgtgggccg
      541 agccgacctg ggccgcctcc tgcccgcggc tcggcggcca gagggccaac tggccctgcc
      601 gctgcaggtt ggtggcctgg gtgtcgatgg cgcggatgat caccatgtcg cggtagaact
      661 gctcgagctc ggcgtcggtc agggcgtcga tgaccggcag gtactgctcg gatgccggcg
      721 acggagcgaa tgatccatcc gcttccagga cgcgaacgag gggtgtttcg gtcgtggtca
      781 cggctccacg ctacccacgt cggcatgccc cgtcacgcat agttcggggg ccctagtaag
      841 tggccgggat ctggataaac gctcgcgctc agcgcccgtg cgcctgcgcg agcctctcca
      901 gcgccggggc gatcgcgtga cgcggggcgg cgaggttcat gcgttcgaac ccctcgccct
      961 cgggaccgaa catggcgccc tcgtcgaaga acaccagcgc ctcctgctcg tggatgcgct
     1021 tgagctcctc gtgcgtgcag tccagcccgc ggaagtccat ccactgcagg tacgtgccct
     1081 ccaggtcgta gacggtgacc tcgggcatcc gctcggcgag gaacgaccgc accagctcgt
     1141 ggttgccgtg caccaggtcg atcaggccgt cgagccagcc ctccgccttc gtgtacgcca
     1201 gccggcacgc ctggaatccc agggcgttca gcgtgaagaa tccggtgcgg ctctgctctg
     1261 cggcgtactt ctcgcgtagc tcggggttcg ggatgacgat gttgctcgtc gccatgcctg
     1321 cgaggttgaa cgtcttgctg ggcgcggtgc acacgatcgt gcgcgcggcg gcatcctcgt
     1381 tcagcgtgga gaagacggtg tgccggatgc cgggcatcag caggtcgaag tggatctcgt
     1441 cgctgatgac ggtgaggtcg ttgtcgcggg cgatgcgcca gacggcctcg agctcctcac
     1501 gctcccagac ccggccggtg gggttgtgcg ggctgcagaa cagcagcgcg gtggtgcgcg
     1561 ggtcagcggc cttcgcggcg aggtcgtcga ggtcgaggcg ccagcgaccg tcctgcagca
     1621 cgagcgggtt gcgcacgacg acgcggtcgt tcccctcgat cgcgccgaag aacgggtagt
     1681 acgccggggt ctgcacgatc acgccgtcac cgggcgcggt cacggcccgc acagcggtgt
     1741 tgaacgcggg gaccacgccc ggcgccagca cgatctgctc gcgatcgacc gtccagtcat
     1801 ggcgccgctg gaaccatccg gtcacggcat cccagtagtc atcggtcgcg acggtgtagc
     1861 cgaggacgac gtcaccgagg tggtcgcgca gcccgtccag aatctcgggg gcgagcttca
     1921 ggtcgatgtc ggcgaccgag aacggcgaga tgcccgcagg cacgtcgggg ttcgcggtca
     1981 gcatgtgctg ccacttggac gcaccggtgc cggtccggtc gacgaccgtc tcgaagtcgt
     2041 acgtcatgca cgcacctgcg ccgcggccac ggcgagcacc ttgtcgagcg attcctcctc
     2101 gccgacggag atgcggatgc cgtcgcccga gaacggccgc acgatcaggt cggcggcgtc
     2161 gaacgctgcg gcgacctcat cggtgcgctc gcccgagggc agccacacga agttggcctg
     2221 gctgtccggc acgtcccagc cctgcgcgcg cagggctgcg atcaggcggg tgcggcgctc
     2281 gacgatcacg gag
//
LOCUS       DATMSX010000479         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_121478,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000479 DATMSX010000000
VERSION     DATMSX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121478"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VN041_15265"
     CDS             <1..1122
                     /locus_tag="VN041_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWU30440.1"
                     /translation="VAASAGTTPPATPSGRPGPKARELRFRPDGTFKVVQFNDTQDDE
                     LTDRRTVELIEKVLDQEKPDFVVINGDVISGGCASRLEVKQAINNVVWPMESRRIPWA
                     VTYGNHDEDSLPESGVDEAGMLKIYRSYDFNVNADNAAGLTGTSNTIVSIASSAKKNR
                     DAFALWLIDSGRYAPGAINGQNFDGYPTWDWIRMDQVAWYREQSQQLEKRLGRKVPGL
                     MFIHIALWEHRFMWWGGVDTRTAADAARGKARHGIVGERNEDECPGPINSGMYNAILE
                     RGDVKGVFVGHDHVNDYVGDYYGVMLGYAPGTGFGAYGLDGAERNRLRGGRVFELTEN
                     GDDVSIKTRVVYARDLGIDLTANDQPMEPLPLAPKQERI"
     gene            complement(1186..1863)
                     /locus_tag="VN041_15270"
     CDS             complement(1186..1863)
                     /locus_tag="VN041_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HWU30441.1"
                     /translation="MSEPISIDIWSDIACPWCYIGKRNLEAGLASTAGDPDAPQVEIT
                     FHSFELSPDTPVDFDGDELDFLAGHKGMAREQVEQMLSHVTKVAAGAGLQYRFDLLQH
                     TNTVKAHELLHFAKAKGLQHEMEERLMSAYFTEGKHVGRVDDLVALAAEVGLDADETR
                     SALESAEYLDAVRADQAQARAYGINGVPFFVIDGQYGVSGAQPPEAFENVIRDIWAQK
                     QGASAGA"
     gene            1919..2488
                     /locus_tag="VN041_15275"
     CDS             1919..2488
                     /locus_tag="VN041_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU30442.1"
                     /translation="MLSASDPIPFRPRRVLVAGVSGSGKTTFAARLADAVDLPRYELD
                     ALHHGPEWTPRESFLDEVRAFAATDRWISEWQYTSRGVDDLLPPRAELCAWLDYPDRI
                     VRGRLWRRTLTRSILRRELWNGNREPTLWQLATTTAPEENVLRWQQQTLNVWRERMPE
                     YEARFPHLTIVRFAHPREAEEWLSRVPAG"
     gene            complement(2501..3304)
                     /locus_tag="VN041_15280"
     CDS             complement(2501..3304)
                     /locus_tag="VN041_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWU30443.1"
                     /translation="MTPKPYTHKDAVPFRPLDWTGAHPPQFPAVPNHVAIVMDGNGRW
                     ANRRGLSRIEGHKAGEEVLLDVVAGAIQAGVKHLSVYAFSTENWARSPEEVRFLMGYN
                     RDVLHRRRDQLNEWGVRVRWAGRKPRLWGSVIKELQFAEQLTRGNDVLTLTMCVNYGG
                     RVELVDAMRALADEVKAGRLKPSAITEKQIRRHLYVPDMPDVDLFLRSSGEQRTSNFL
                     LWQSAYAEMVFLDTLWPDFSREELWRAIGIYLSRDRRFGGAVDAPDASE"
     gene            complement(3301..>3570)
                     /locus_tag="VN041_15285"
     CDS             complement(3301..>3570)
                     /locus_tag="VN041_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HWU30444.1"
                     /translation="ISHSHFGPQMGGTVCSDCAPAGSPRISASTLALLRALMGGDWAT
                     VDAAAPGDLAAASGTVAAYVQYQLERGIRSLEHVTSISSDRQGIR"
BASE COUNT          566 a         1213 c         1244 g          547 t
ORIGIN      
        1 gtggccgcga gtgccggcac gaccccgcct gccacgccgt ccggccggcc cggcccgaag
       61 gcgcgcgagc tgcggttccg tcccgacggg accttcaagg tcgtgcagtt caacgacacg
      121 caggacgacg agctcaccga ccggcgcacg gtcgagctga tcgagaaggt gctcgaccag
      181 gagaagcccg atttcgtggt gatcaacgga gacgtcatct ccggcggctg cgcgtcgcgc
      241 ctcgaagtca agcaggcgat caacaacgtg gtctggccga tggagtcgcg gcgcatcccg
      301 tgggcggtca cctacggcaa ccacgatgag gactcgctgc ccgagtcggg tgtcgacgag
      361 gccgggatgc tgaagatcta ccgctcgtac gacttcaacg tgaacgccga caacgcggcg
      421 ggcctgaccg gcacgagcaa cacgatcgtg tcgatcgcct cgtccgcgaa gaagaaccgc
      481 gatgcgttcg cgctgtggct gatcgactcc ggccgctacg cccctggcgc gatcaacggc
      541 cagaacttcg acggctaccc cacctgggac tggatccgca tggatcaggt ggcctggtac
      601 cgcgagcagt cgcagcagct ggagaagcgg ctgggccgca aggtccccgg cctcatgttc
      661 atccacatcg cgctgtggga gcaccgcttc atgtggtggg gtggcgtcga cacccgcacg
      721 gcggcggatg ccgcgcgcgg caaggcgcgt cacggcatcg tcggtgagcg caacgaggac
      781 gagtgccccg gtccgatcaa ctcgggcatg tacaacgcca tcctcgagcg cggcgatgtg
      841 aagggcgtgt tcgtgggtca cgaccacgtc aacgactacg tgggcgacta ctacggcgtg
      901 atgctcggat acgcgcccgg caccggcttc ggcgcctacg gtctggacgg cgctgagcgc
      961 aaccgcctcc gcggcggccg ggtcttcgag ctgaccgaga acggcgacga cgtgtcgatc
     1021 aagacgcgcg tcgtgtacgc acgcgacctg ggcatcgacc tcacggcgaa cgaccagccg
     1081 atggagccgc tgccgctggc tccgaagcag gagcggatct gacgctcccc tgcgacgagt
     1141 gcggcgggtc ccggaaaggg gcccgccgca ctcgtgatcg gtggctcagg cccccgcgga
     1201 agcgccctgc ttctgcgccc agatgtcgcg gatcacgttc tcgaacgcct ccggcggctg
     1261 cgcgccgctg acgccgtact ggccgtcgat cacgaagaac gggacgccgt tgatgccgta
     1321 ggcgcgcgcc tgcgcctggt cggcacggac ggcgtccagg tactcggcgg actccagagc
     1381 ggagcgggtc tcatccgcgt ccagtccgac ctcggcggcg agggcgacca ggtcgtcgac
     1441 gcggccgacg tgcttgccct cggtgaagta ggcggacatg agccgctcct ccatctcgtg
     1501 ctgcagaccc ttcgccttgg cgaagtgcag gagctcatgc gccttgaccg tgttggtgtg
     1561 ctgcagcaga tcgaagcggt actgcaggcc cgcgccggct gcgaccttgg tcacgtgcga
     1621 cagcatctgc tcgacctgct cccgcgccat gcccttgtgc ccggcgagga agtcgagctc
     1681 gtcgccgtcg aagtcgacgg gggtgtcggg cgagagctcg aaggagtgga aggtgatctc
     1741 cacctgcggg gcgtcgggat cccccgccgt ggaggcgaga cccgcctcga ggttgcgctt
     1801 gccgatgtag caccaggggc aggcgatgtc ggaccagatg tcgatggaga tgggttcgct
     1861 cacaacacca ttcaacggca tggaacgtga tcgtattccg tgccggtaat cttctctggt
     1921 gctcagcgcc tccgatccga tccccttccg tccgcggcgg gtgctcgtcg ccggagtctc
     1981 cggatccggg aagacgacgt tcgccgcccg gcttgcggat gccgtggatc tgccgcgcta
     2041 cgagctcgat gcgctgcatc acggcccgga gtggacgccg cgggagtcgt tcctcgacga
     2101 agtgcgcgcg ttcgccgcga cggaccgctg gatcagcgag tggcagtaca cgtcgagggg
     2161 cgtcgacgac ctgctgccgc cgcgagccga gctgtgcgcg tggctcgact accccgaccg
     2221 gatcgtgcgc gggcgcctgt ggcgccggac gttgacgcgc agcatcctgc ggcgggagct
     2281 gtggaacggc aaccgtgaac cgacgctctg gcaattggcg accacgaccg cgcccgagga
     2341 gaacgtgctg cgctggcagc agcagacgtt gaacgtgtgg cgggagcgga tgccggagta
     2401 tgaggcgcgc ttcccgcacc tgacgatcgt gcgcttcgcg catccgcgcg aggcggagga
     2461 gtggctcagt cgggtgccgg cgggctgagc ccgcacgccg ctactccgac gcgtccggcg
     2521 cgtccacggc cccgccgaac cggcgatcgc gggacaggta gatcccgatc gcgcgccaca
     2581 gctcctcgcg ggagaagtcc ggccacagcg tgtccaggaa caccatctcg gcgtacgcgg
     2641 actgccagag caggaagttc gaggtgcgct gctcgcccga gctgcgcagg aacaggtcga
     2701 cgtcgggcat gtccggcacg tacaggtgcc ggcggatctg cttctcggtg atggcgctcg
     2761 gcttcagccg cccggccttc acctcgtccg cgagagcgcg catcgcgtcc acgagctcca
     2821 cacgaccgcc gtagttgacg cacatcgtca gcgtgagaac gtcgttcccg cgggtcagct
     2881 gctcggcgaa ctgcagctcc ttgatcaccg atccccacag ccggggcttg cgccccgccc
     2941 aacgcacccg cacgccccac tcgttgagct gatcgcgccg gcggtgcagc acgtcgcggt
     3001 tgtagcccat caggaagcgc acctcctcgg gtgagcgcgc ccagttctcc gtggagaacg
     3061 cgtacaccga gaggtgcttg acgccggcct ggatggcgcc ggcgacgacg tcgagcagca
     3121 cctcctcacc ggccttgtga ccctcgattc ggctgagccc cctgcggttc gcccagcgtc
     3181 cgttgccgtc catgacgatc gcgacgtggt tcggcactgc ggggaactgc ggcgggtggg
     3241 ccccggtcca gtccagcggg cggaacggca ccgcgtcctt gtgcgtgtag ggcttcgggg
     3301 tcaccggatt ccttgtcggt cggaagagat ggaggtcaca tgctccaggg atcggatgcc
     3361 gcgctccagc tgatactgca cataggccgc caccgtgcct gacgccgcgg cgaggtcgcc
     3421 cggagcagca gcatccacgg tcgcccagtc gccgcccatc agggcacgca gcagcgcgag
     3481 tgtcgacgcc gagatccgcg ggcttcccgc cggggcgcag tcgctgcaga cggtcccgcc
     3541 catctggggc ccgaaatgcg agtgcgagat
//
LOCUS       DATMSX010000480         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_26190,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000480 DATMSX010000000
VERSION     DATMSX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26190"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="hemE"
                     /locus_tag="VN041_15290"
     CDS             complement(<1..864)
                     /gene="hemE"
                     /locus_tag="VN041_15290"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWU30445.1"
                     /translation="MSDLLRALAGESVERTPVWFMRQAGRSLPEYRELRVGTRMLDAC
                     LTPDVAAEITLQPVRRHGVDAAVFFSDIVIPLRLAGVEVEIEPGRGPVFANPVRTAED
                     VARITAIDPADLDGTAIAEAVRIVTAELGETPLIGFAGAPFTLAAYLIEGGPSKEHLR
                     ARAMMHADPESWDRLAGWLSRVSRRFLEIQRDAGASVVQLFDSWAGSLSPADYRAHIA
                     PHSHAALDGIGLPSIHFGVGTGPFLGDMRLDGVASAVGVDWRMPLDEAIAVLGPDVAV
                     QGNIDPALLQAR"
     gene            954..2255
                     /locus_tag="VN041_15295"
     CDS             954..2255
                     /locus_tag="VN041_15295"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWU30446.1"
                     /translation="MLLCVTASHKTASFELLERLSRHPETVAPTLVEAGAQGAVVLAT
                     CNRFEAYVESDGLDTDAVLDVVAQATGIPSADLDGSYRVIDDRRVAEHLFAVASGLES
                     VVSGEGEIAGQVRRALTGARKQGTTSPALERLFQRASQAQRKVKNVTALQRAGRSLVR
                     LSLELADSRIADWAAERVLLVGTGAYAAVTLATLRERGAVDISVYSPSGRAELFAKKH
                     GIRPVFDDDYAAVAQRSSLLITCTTATEPVLGPAHLQRITAAQVADAKNGCPVASPGS
                     TPHVSSQLVIDLGMPRNVDPAIAQLEGVALLDLETIRLHAPLEELQATDAARAIVHEA
                     AEDFHAVGSRATFTPGVVALRTHIFGLLEAEIERARNRGDENGLVEQAMRHLAGVLLH
                     TPTTRAHELAADGRGGEFLSALETLYGLVPDAGEATESEIA"
     gene            2302..2709
                     /gene="arr"
                     /locus_tag="VN041_15300"
     CDS             2302..2709
                     /gene="arr"
                     /locus_tag="VN041_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HWU30447.1"
                     /translation="MSDALDDGPFFHGTAAALQPGELLTAGFPSNYRPEITMNHVYFT
                     ALRDGAGLAAEIAAVDGDPHVYLVEPTGEFENDPNVTDKKFPGNPTRSYRTREPLRVI
                     EEVHDWTRLTPEALQEWRDRIAAIVETRGEIIN"
BASE COUNT          416 a         1003 c          924 g          366 t
ORIGIN      
        1 tctcgcctgc agcagagcgg ggtcgatgtt gccctgcacg gccacgtcgg ggccgagcac
       61 ggcgatggcc tcgtccagcg gcatccgcca gtcgacgccc acggccgagg cgacgccgtc
      121 caggcgcatg tcgccgagga agggtccggt ccccacgccg aagtggatgc tcggcagccc
      181 gatcccgtcg agcgcggcgt gcgagtgcgg cgcgatgtgc gcgcggtagt cggcggggct
      241 gagggaaccc gcccaggagt cgaacagctg cacgacggat gctccggcgt cgcgctggat
      301 ctcgaggaag cggcgcgaga cgcgggagag ccagcccgcc agccggtccc acgactccgg
      361 gtcggcatgc atcatggcgc gggcgcgcag gtgctccttg gacggcccgc cctcgatgag
      421 gtatgcggcg agcgtgaacg gggcgccggc gaagccgatc agcggggtct cccccagctc
      481 cgcggtgacg atgcgcacgg cctccgcgat cgcggtgccg tcgaggtcgg cggggtcgat
      541 ggcggtgatg cgcgccacgt cttcggcggt gcgcaccgga ttcgcgaaca ccggcccgcg
      601 gcccggctcg atctccacct cgacgcccgc caggcgcagc gggatcacga tgtcgctgaa
      661 gaacaccgcg gcgtccacgc cgtgccggcg caccggctgc agggtgatct cggcggccac
      721 gtccggggtg aggcaggcat ccagcatccg cgtgccgacg cgcagctcgc ggtactcggg
      781 cagcgaacgg cccgcctgac gcatgaacca gacgggggtg cgctcgacgg attcaccggc
      841 gagcgcgcgc agcagatcac tcatgctccg atcctttcac cgtcaaccta tgcggatgct
      901 cggttaggca cccctcagat gccgatcaca gataccaggc gtaccctgga gaggtgctgc
      961 tctgcgtcac ggcgagtcac aagaccgcct ccttcgaact gctcgagcgc ctcagccgcc
     1021 atcccgagac cgttgccccg accctggtcg aagccggtgc gcagggagcc gtcgtgctcg
     1081 ccacctgcaa ccgcttcgag gcctacgtcg agagcgacgg gctcgacacg gatgccgtgc
     1141 tggacgtcgt cgcgcaggcc accggcatcc cctccgccga cctcgacggc tcgtaccgcg
     1201 tgatcgacga ccgacgcgtc gccgagcacc tgttcgccgt ggcatccggc ctggagtcgg
     1261 tcgtctccgg cgagggcgag atcgccggcc aggtgcgccg agctctgacc ggcgcccgca
     1321 agcagggcac cacctccccc gcgctggagc gactcttcca gcgggcaagt caggcacagc
     1381 gcaaagtcaa gaacgtcacg gctctgcagc gcgccggccg ctcgctcgtg cggctctcgc
     1441 tggagctcgc cgacagccgc atcgccgact gggccgccga gcgcgtgctg ctggtcggca
     1501 cgggcgccta cgccgccgtc accctcgcga cgctgcgcga gcgtggtgct gtcgacatct
     1561 cggtctactc accctcgggc cgggcggagc tgttcgccaa gaagcacggc atccgaccgg
     1621 tgttcgacga tgactacgcc gccgtcgcgc agcgctcctc gctgctgatc acctgcacca
     1681 ccgccaccga gccggtgctg ggtccggcgc acctgcagcg gatcacggcc gcgcaggtcg
     1741 cggacgccaa gaacggatgc cccgtcgctt cccccggctc aacgccccat gtctcgtcgc
     1801 agctggtgat cgacctgggg atgccgcgca acgtcgaccc cgcgatcgca cagctcgagg
     1861 gcgtcgcgct gctggacctc gagacgatcc ggctgcacgc gccgctcgag gagcttcagg
     1921 cgacggatgc cgcccgcgcg atcgtgcacg aggcggccga ggacttccac gccgtcggct
     1981 cccgcgcgac cttcaccccc ggcgtcgtcg cgctgcgcac ccacatcttc ggtctgctcg
     2041 aggccgagat cgagcgcgcg cgcaaccgcg gcgatgagaa cggcctggtc gaacaggcca
     2101 tgcggcacct cgccggcgtg ctgctgcaca ccccgaccac gcgcgcgcac gagctcgcgg
     2161 ccgatggtcg cggcggcgag ttcctcagcg cactcgagac gctgtacggc ctggttccgg
     2221 atgccggtga ggccacggaa tccgagatcg cctgacgcac ggcatccgcc cctccgaccg
     2281 catctgagtg acactggatg catgagcgac gcactggatg acggaccctt cttccacggc
     2341 accgcggcgg ccctgcagcc cggcgagctg ctgaccgcgg gcttcccctc gaactaccgg
     2401 cccgagatca cgatgaacca cgtgtacttc accgcgctgc gcgacggagc gggtctcgcc
     2461 gccgagatcg ccgctgtcga cggcgacccg cacgtctacc tggtcgagcc gacgggcgag
     2521 ttcgagaacg acccgaacgt caccgacaag aagttccccg gcaatccgac ccgctcgtac
     2581 cgcacgcgcg agccgctgcg cgtgatcgag gaggtgcacg actggacgcg cctcacgccc
     2641 gaggcgctgc aggagtggcg cgaccggatc gcggcgatcg tcgagacccg cggcgagatc
     2701 atcaactga
//
LOCUS       DATMSX010000481         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_93010,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000481 DATMSX010000000
VERSION     DATMSX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93010"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..>2068)
                     /locus_tag="VN041_15305"
     CDS             complement(76..>2068)
                     /locus_tag="VN041_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C14"
                     /protein_id="HWU30448.1"
                     /translation="PMARDAGSASTVASLQKVEAKADDIVAVAGYAEPGDGGGGLFRC
                     VTADQPKVDGGTVVAGKGGDVWVRVHSGTMDFRAFGIFGPDVPADDALDAMVADAGIH
                     RIEAHTDLNFVKRHRFSRSNIVFDFGNHLMTTEGIENAGKDDPFAAVMFFQGELTGEE
                     RTAVLGAPVADLADVYPVGDSSFFQVGEWYAAEVNALSGRWERELQRLVQVTQIVDGS
                     RIRVNYQNGWPLGKDRSITWRHVKPVQNVVVSNLRFRGKGTDEYTGSHPLAFEYAVRC
                     DVDHIDGTATFWPLIMRRWNTYFTTESCTLKNPTSVTWGGAGYMTQQIYCLYGYVANC
                     HTSNARHLNDWTASAYGLVENCHGDGDDQGPFVTHGQYEHDLTYVGNSGLMTFANSGA
                     AWGGAAKRITVRKHVCSWFVARVKVTDLTLEDMRVIGKPDLAGSGMLWINADGAQLRG
                     CSATDTLIITQASDASKRPTVIEASEFMFVAAGDLTNATVTKPVTFVDTRLNGVNGLK
                     LGGAGVVEFRGGQLIGGDRDAAFSTSSARLRLTGVEADGIRIDAASGKAQAVEILGGS
                     AVRTAAGTAVSHSGGGELTVVLGDSTFSGKGTHVSLTSGANHYRATGCRFDGGTLELA
                     AAAFGAGSTLLHSANVETAVKRVVPDAADNVLTTGNVIV"
BASE COUNT          308 a          746 c          690 g          324 t
ORIGIN      
        1 ggcatccgcc gcgaccgccg gccgcaggat cgggtcagcg ggcatccggc cccgcaccct
       61 tcacgaaccg tccgctcaca cgatcacgtt cccggtggtc aggacgttgt ccgccgcgtc
      121 ggggacgacg cgcttcaccg ccgtctcgac gttggcggag tgcagcagcg tgctgcccgc
      181 gccgaacgcc gctgcggcga gctccagggt gccgccgtcg aatcggcatc cggtggcccg
      241 gtagtgattc gcgcccgagg tgagcgagac gtgcgtgccc ttgcccgaga acgtgctgtc
      301 gccgagcacg acggtcagct ccccgccgcc gctgtgcgac accgccgtac ccgccgcggt
      361 gcgcaccgcg gatccgccga ggatctcgac cgcctgcgcc ttgccgctcg cggcgtcgat
      421 gcggatgccg tcggcctcga cgccggtgag ccgcagacgg gccgacgagg tgctgaaggc
      481 tgcatcgcgg tcgccgccga tcagctgccc accgcggaac tcgacgacgc cggcgccgcc
      541 gagcttcagc ccgttcacgc cgttcagccg ggtgtcgacg aacgtcaccg gcttggtcac
      601 ggtggcgttg gtcagatcac ccgccgccac gaacatgaac tcggacgcct cgatcacggt
      661 cggccgcttc gacgcatccg atgcctgcgt gatgatcagc gtgtcagtgg cggagcagcc
      721 gcgcagctgc gcgccgtcgg cgttgatcca gagcatgccg gagcccgcca ggtcgggctt
      781 gccgatcacc cgcatgtcct cgagggtcag gtcggtgacc ttcacccgcg cgacgaacca
      841 ggagcagacg tgcttgcgca ccgtgatgcg cttcgccgcg ccgccccagg ccgccccgga
      901 gttggcgaac gtcatcaggc cggagttccc cacgtaggtg aggtcgtgct cgtactgccc
      961 gtgcgtcacg aacgggccct gatcgtcgcc gtcgccgtgg cagttctcga ccaggccgta
     1021 ggcactggcg gtccagtcgt tcaggtgccg ggcgttcgag gtgtggcagt tcgccacgta
     1081 gccgtacagg cagtagatct gctgcgtcat gtagcccgcc ccgccccacg tgaccgaggt
     1141 cgggttcttc agcgtgcagc tctcggtggt gaagtaggtg ttccaccggc gcatgatcag
     1201 gggccagaag gtggcggtgc cgtcgatgtg atccacgtcg cagcgcaccg cgtactcgaa
     1261 ggccagcggg tgggagccgg tgtactcgtc cgtgcccttg ccgcggaagc gcaggttcga
     1321 caccaccacg ttctgcacgg gcttcacatg ccgccacgtg atcgagcggt ccttgccgag
     1381 gggccagccg ttctggtagt tcacgcggat gcgggagccg tcgacgatct gcgtcacctg
     1441 cacgagccgc tgcagctcgc gctcccagcg gccggagagg gcgttcacct cagcggcgta
     1501 ccactcgccg acctggaaga acgacgagtc gcccacgggg tagacgtcgg ccagatccgc
     1561 gaccggcgca ccaagcacag cggtgcgctc ctcaccggtc agctcgccct ggaagaacat
     1621 cacggcggcg aacgggtcgt ccttgcccgc gttctcgatc ccctcggtcg tcatcaggtg
     1681 attgccgaag tcgaacacga tgttcgaccg cgagaacctg tgccgcttca cgaagttcag
     1741 atccgtgtgg gcctcgatgc gatggatgcc cgcatccgcc accatcgcgt ccagagcgtc
     1801 gtccgcgggg acatccggcc cgaagatgcc gaacgcgcgg aagtccatcg tcccggagtg
     1861 cacgcgcacc cagacatcgc cacccttgcc ggcgacgacc gttccaccgt cgaccttcgg
     1921 ctgatcggcg gtcacgcagc ggaagaggcc gccaccgccg tcacccggct cggcgtagcc
     1981 cgcgaccgcg acgatgtcgt cggccttggc ctcgaccttc tgcagcgagg cgaccgtcga
     2041 cgccgacccg gcgtccctcg ccatcggc
//
LOCUS       DATMSX010000482         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_130889,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000482 DATMSX010000000
VERSION     DATMSX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130889"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VN041_15310"
     CDS             <1..512
                     /locus_tag="VN041_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU30449.1"
                     /translation="LGRLRRRGRPAPVVPSIDFTEATDAPVRTPPIVLGEENKVRQVV
                     TNLLGNARRFSPAESPIELVVGADRRRGVGTIAIVDHGEGIPPQIREHIFERFWRADT
                     SRARETGGAGLGLAIVASILESLHGSVHVEETPGGGATFVVSLPLAPAQATPAHLLEE
                     TQPLDRLDL"
     gene            629..916
                     /locus_tag="VN041_15315"
     CDS             629..916
                     /locus_tag="VN041_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HWU30450.1"
                     /translation="MSVFTVDTDAVHAANGATRATMERLQSESAALMAQLTQLQSSWT
                     GSASLAFQQCSEQWRGAQLHVEQVLASIGTALGSAASQYADADQYSASLFR"
     gene            complement(988..2604)
                     /gene="groL"
                     /locus_tag="VN041_15320"
     CDS             complement(988..2604)
                     /gene="groL"
                     /locus_tag="VN041_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039166.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWU30451.1"
                     /translation="MAKIIAFDEEARRGLERGLNILADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGADPISLKKGIEKAVAAITADLLENAKEIESKEQIAATASISAADPEIGALI
                     AEAIDKVGKEGVVTVEESQTFGTELELTEGMRFDKGYLNPYFVTDPERQEAVFEDPYI
                     LIANQKIGNIKDLLPVVDKVIQDGKELVIIAEDVEGEALATLVLNKIRGIFKSVAVKA
                     PGFGDRRKAQLQDIAILTGGQVITEEVGLKLENTTLDLLGRARKVIVTKDETTIVEGA
                     GEQSAIEGRVTQIRREIENTDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVPGGGVALLQSAKVLDTLTLEGDEATGANIVRVAIEAPLKQ
                     IAINAGLEPGVVAHKVSELPAGQGLNAATGEYGDMFAQGIIDPAKVTRSALQNAASIA
                     GLFLTTEAVVADKPEKAPAMPADPSGGMDF"
     gene            complement(2763..3350)
                     /locus_tag="VN041_15325"
     CDS             complement(2763..3350)
                     /locus_tag="VN041_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HWU30452.1"
                     /translation="MSKSPHDRFDDIPRTSGRVGAHRAEQPGINGPIALLWSAAVALV
                     LIVIGIFVSMVLMGRIDLFGTAEPAAPQTPGVLAEIDTSYQVLILNATPETGVVAQVR
                     QKLIDQGWAADAVFGSDGSTTKFDQTTVFYVKKADESAALGVAEVLGGAKTEQSDHYQ
                     SVSGDGSPQLTVVIGMDHVGTTSPTPGGGEKTPAG"
     gene            complement(3399..3656)
                     /locus_tag="VN041_15330"
     CDS             complement(3399..3656)
                     /locus_tag="VN041_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HWU30453.1"
                     /translation="MLGELSDRDRAILTLEAAWPRHGGMKEETIRAQLGMSPARYYQL
                     LGRLIDTEEALEFDPLLVRRLRRLRDARGRQRTARMRGFAG"
     gene            complement(3666..>3979)
                     /locus_tag="VN041_15335"
     CDS             complement(3666..>3979)
                     /locus_tag="VN041_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HWU30454.1"
                     /translation="AAQRLGELVASAPPQATLVITTPGVLVYLPREARKALIGAIRSS
                     GGRWVTIDPVRLQEGWSSGEGWSGVDGAFAVGLDGDQVADADPLGRWWEWRAGVGPDR
                     A"
BASE COUNT          549 a         1411 c         1352 g          667 t
ORIGIN      
        1 cgctgggccg actgcgccgc cgcggaaggc ccgcaccggt cgtgccgtcg atcgacttca
       61 ccgaggcgac ggatgctccg gtccgcacgc ccccgatcgt gctcggcgag gagaacaagg
      121 tgcgtcaggt cgtgacgaac ctgctcggca acgcgcgccg cttctccccc gccgagagcc
      181 ccatcgagct cgtcgtcggc gcggaccgcc gccgcggcgt cggcacgatc gcgatcgtgg
      241 atcacggcga gggcatcccg ccgcagatcc gcgagcacat cttcgagcgc ttctggcgcg
      301 ccgacacgtc ccgcgcacgg gagaccggcg gggcgggcct gggcctggcg atcgtcgcct
      361 ccatcctgga gtcgctgcac ggctccgtgc acgtcgagga gacgcccggg ggcggcgcga
      421 ccttcgtcgt ctcccttccg ctcgcccccg cacaggcgac cccggcgcat ctgctcgagg
      481 agacccagcc gctggaccgc ctggacctct gagcgacttc ccctgcacat ctcccgtccg
      541 ccacgggttg tgcacggatg ccggtgtccc ggcatccgct tccccctccg cgcttctagt
      601 ctcggaagcc caccgagagg agaaccccat gtccgtcttc accgtcgaca ccgatgcggt
      661 ccacgccgcc aacggcgcca cccgcgccac catggagcgt ctgcagagcg aatcggccgc
      721 cctcatggcg cagctcacgc agctgcagtc gtcctggacc ggctccgcgt cactggcgtt
      781 ccagcagtgc agcgagcagt ggcgcggcgc acagctccac gtcgagcagg tgctggcctc
      841 catcggcacc gcgctcggat ctgccgccag ccagtatgcg gatgccgatc agtactccgc
      901 gagcctgttc cgctgagcca tcgccccaga cgagcagaac gccccctgcc ggagcagggg
      961 gcgttctgtc tgtcgatcag gccggactca gaagtccatg ccgcccgacg ggtcagccgg
     1021 catggccgga gccttctcgg gcttgtccgc gacgacggcc tccgtggtga ggaacaggcc
     1081 ggcgatcgag gctgcgttct gcagcgccga gcgggtgacc ttggccgggt cgatgatgcc
     1141 ctgtgcgaac atgtcgccgt actcgccggt cgcggcgttc aggccctggc ccgcggggag
     1201 ctcggagacc ttgtgcgcga ccacacccgg ctcgaggccg gcgttgatgg cgatctgctt
     1261 gagcggcgcc tcgatggcga cgcggacgat gttcgcgccg gtggcctcgt cgccctcgag
     1321 ggtgagggtg tcgagcacct tggccgactg cagcagcgcg acgccaccac cggggacgat
     1381 gccctcctcg acggcagcct tcgcgttgcg gacggcgtcc tcgatgcggt gcttgcgctc
     1441 cttgagctcg acctcggtgg ccgcgcccgc cttgatgacg gccacgccgc cggcgagctt
     1501 ggcgaggcgc tcctgcagct tctcgcggtc gtagtcgctg tcggtgttct cgatctcgcg
     1561 acggatctgg gtcacgcggc cctcgatcgc ggactgctca ccggcaccct cgacgatcgt
     1621 ggtctcgtcc ttggtgacga tgaccttgcg ggcgcgtccg agcaggtcga gggtggtgtt
     1681 ctcgagcttg agaccgacct cctcggtgat gacctggccg ccggtcagga tcgcgatgtc
     1741 ctgcagctgg gccttgcgac ggtcaccgaa gcccggagcc ttgacggcga cggacttgaa
     1801 gatgccgcgg atcttgttca gcaccagcgt cgcgagagcc tcgccctcga cgtcctcggc
     1861 gatgatgacg agctccttgc cgtcctggat caccttgtcg acgaccggca gcaggtcctt
     1921 gatgttgccg atcttctggt tggcgatgag gatgtacggg tcctcgaaga cggcctcctg
     1981 gcgctccggg tccgtgacga agtacgggtt caggtagccc ttgtcgaagc gcatgccctc
     2041 ggtgagctcg agctcggtgc cgaaggtctg cgactcctcg acggtgacga caccctcctt
     2101 gccgaccttg tcgatcgcct cggcgatgag cgcgccgatc tcggggtcag cggcggagat
     2161 cgacgcggtg gccgcgatct gctccttgga ctcgatctcc ttggcgttct ccagaaggtc
     2221 ggcggtgatg gccgcgacgg ccttctcgat gcccttcttc aggctgatcg ggtcggcgcc
     2281 ggctgcgacg ttgcgcagac cctcgcggac cagtgcctga gcgaggacgg tcgcggtggt
     2341 ggttccgtca ccggcgacgt cgtcggtctt cttggcgacc tccttgacga gctccgcacc
     2401 gatcttctcg tacgggtcgt cgagctcgat ctccttggcg atggagacgc cgtcgttcgt
     2461 gatcgtgggg gcgccccact tcttctccag tacgacgttg cgaccgcgcg ggccgagggt
     2521 caccttgacg gcgtcggcca ggatgttcag gccgcgctcg aggccgcggc gggcctcctc
     2581 atcgaaagcg atgatctttg ccatgagtct ttcgtccctc ccggacgtag gcgatgtggt
     2641 tagcactcag ggtgatcgag tgctaacact cagtctggca ctcgacccat gagagtgcaa
     2701 gccgcgtccg ccgagcgcgg atcaggccac ggattcggcc cgtgggggca gcgcgtgagg
     2761 ggtcagcccg cgggggtctt ctccccgccg ccgggtgtgg ggctcgtcgt gccgacgtgg
     2821 tccatgccga tcacgacggt cagctgaggg gagccgtcgc cgctgaccga ctggtagtgg
     2881 tcgctctgct cggtcttcgc gccgcccagg acctccgcga cgccgagcgc tgcggactcg
     2941 tccgccttct tcacgtagaa gacggtggtc tggtcgaact tcgtggtgga gccgtcgctg
     3001 ccgaacacgg catccgccgc ccagccctga tcgatcagct tctgacgcac ctgggcgacc
     3061 acaccggtct ccggggtcgc gttgaggatg agcacctggt acgaggtgtc gatctcggcc
     3121 agcacgccgg gcgtctgggg agctgcgggc tcggccgtgc cgaacaggtc gatgcgcccc
     3181 atcagcacca tcgagacgaa gatcccgatg acgatgagca ccagcgcgac cgccgccgac
     3241 cacagcagag cgatgggtcc gttgatgccc ggctgctcgg cgcggtgcgc gccgacgcgt
     3301 ccggaggtgc gggggatgtc gtcgaatcgg tcgtgtgggg actttgacac cccacgatgc
     3361 tatcggccga accccgggaa accgctggag cggtcggctc agccggcgaa tccgcgcatg
     3421 cgcgccgtgc gctgccggcc tcgggcgtcc ctcagccgtc gcaggcggcg caccagcaga
     3481 gggtcgaact ccagcgcctc ctcggtgtcg atcaggcgcc cgagcagctg gtaatagcgc
     3541 gccgggctca tgcccaactg cgcgcggatc gtctcctcct tcatgccgcc gtgccgcggc
     3601 cacgccgctt ccagcgtcag gatcgcgcga tcgcgatcgg agagttcacc cagcatggca
     3661 tcaggctacg cgcgatcggg cccgacgccc gcgcgccact cccaccagcg ccccagtggg
     3721 tcggcatccg cgacctgatc gccgtcgagg cccaccgcga aggcgccgtc gaccccggac
     3781 cagccctccc ccgacgacca gccctcttgc aggcgcaccg gatcgatcgt cacccagcgc
     3841 ccaccggacg agcggatggc cccgatcagt gccttacgcg cctcgcgcgg caggtagacg
     3901 agcacgccgg gggtggtgat cacgagggtc gcctgcggcg gtgcggatgc cacgagctcg
     3961 cccagccgct gcgcggcgt
//
LOCUS       DATMSX010000483         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_178080,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000483 DATMSX010000000
VERSION     DATMSX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178080"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VN041_15340"
     CDS             <1..990
                     /locus_tag="VN041_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWU30455.1"
                     /translation="LQVGRPVLPMLAASAATPTAALEITGRASVEYKLDGARIQVHRH
                     GDEVGVFTRSLADITHRVPELVEIVRALPVHDAILDGETLSLDEDGGPRPFQETMSRF
                     GADASRELALRPWFFDVLHLDGRDLIDEPLSTRLAELERVAGEWRMPGIITDDAEAAE
                     QLSREALAAGHEGVVVKAVDSPYSAGRRGKSWVKVKPVLTFDLVVLAAEWGSGRRRGM
                     LSNLHLGALDPVGEFVEPGGLVMVGKTFKGLTDELLRWQTETFPGIESHRTPHAVFLR
                     PEIVVEIAIDGVQRSPRYPGGIALRFARVKGYRPDKKPAEADTIQALRALLRG"
     gene            complement(1010..1519)
                     /locus_tag="VN041_15345"
     CDS             complement(1010..1519)
                     /locus_tag="VN041_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HWU30456.1"
                     /translation="MTDTAIERETLTWDGFGDATRELARNILDSGFVPEVVVAIARGG
                     LLPAGAIAYGLGAKNCGAINMEFYTGIGTVLDAPEVLPPELDMDYLNGRRVLLVDDVA
                     DSGRTLALAVKLLQDKGADVRSVTIYTKPTTIIQPDYSFKDTDLWINFPWSFKGTVVE
                     EDQGLPPSA"
     gene            complement(1524..>1809)
                     /locus_tag="VN041_15350"
     CDS             complement(1524..>1809)
                     /locus_tag="VN041_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30457.1"
                     /translation="PLLATMASELTDAGDGESLGSALRMLSAANLTGLPGLGIPSGEP
                     DGRPIGMHLLGRMYGDAELIAVAHRLESISPWRARVPLDPVISRAPDARE"
BASE COUNT          257 a          634 c          640 g          278 t
ORIGIN      
        1 ctgcaggtgg gccgccctgt gctgccgatg ctcgcggcga gcgccgccac cccgacagcg
       61 gcgctcgaga tcacgggccg cgcctcggtg gagtacaagc tcgacggcgc ccggatccag
      121 gtgcaccggc acggcgatga ggtgggcgtg ttcacccgca gcctcgccga catcacccac
      181 cgggtacccg agctcgtcga gatcgtgcgt gcgcttcccg tgcacgatgc gatcctcgac
      241 ggcgagacgc tgtcgctcga cgaggacggc gggccgcgcc cgttccagga gacgatgtcg
      301 cgcttcggcg cggacgcgtc tcgcgagctc gcgctgcgcc cctggttctt cgacgtgcta
      361 cacctggacg gtcgcgacct gatcgacgag ccgctctcca cccggctcgc cgagctcgag
      421 cgcgtcgcgg gcgaatggcg gatgccgggg atcatcaccg acgacgccga ggccgccgag
      481 cagctgtccc gcgaggccct ggccgccggt cacgaaggcg tcgtcgtgaa ggccgtcgat
      541 tcgccgtaca gcgctggccg ccgcggaaag tcctgggtca aggtcaagcc cgtgctcacg
      601 ttcgacctcg tcgtgctggc cgccgagtgg ggatcgggcc gccgccgcgg gatgctgtcc
      661 aacctgcacc tgggtgcgct cgaccccgtg ggcgagttcg tcgagccggg agggctcgtg
      721 atggtcggca agacgttcaa gggcctcacc gacgagttgc tgcgctggca gaccgagacg
      781 ttccccggta tcgagagcca tcgcacgccg catgcggtct tcctgcgccc cgagatcgtc
      841 gtcgagatcg ccatcgacgg cgtacagcgc tccccgcgct accccggcgg catcgcgctg
      901 cgcttcgctc gcgtcaaggg ctaccgcccc gacaagaagc ccgccgaggc ggacaccatc
      961 caggcgctgc gcgctctgct gcgcggctga cgaccgcgca cgcgaaggct caggcgctgg
     1021 gcggcaggcc ctggtcctcc tcgacgaccg tgcccttgaa ggaccagggg aagttgatcc
     1081 acaggtcggt gtccttgaag gagtagtcgg gctggatgat cgtggtcggc ttcgtgtaga
     1141 tggtgaccga gcggacgtcg gcgcccttat cctgcagcag cttcacggcc agcgcgaggg
     1201 tgcggcccga gtcggcgacg tcgtcgacga gcagcacgcg acgcccgttc aggtagtcca
     1261 tgtcgagctc gggcggcagc acctcggggg cgtccagcac tgtgccgatg ccggtgtaga
     1321 actccatgtt gatcgcaccg cagttcttgg cgccgaggcc gtacgcgatg gcgcccgcgg
     1381 gcagcagtcc gccgcgagcg atcgccacga cgacctcggg cacgaagccg ctgtcgagga
     1441 tgttccgggc gagctcgcgc gtcgcgtctc cgaagccgtc ccaggtgagc gtctcgcgct
     1501 cgatcgctgt atccgtcatt gctctattct cgtgcatccg gtgcccgcga gatcaccgga
     1561 tcgagcggca cacgcgcacg ccagggcgag atcgactcga ggcggtgggc gacggcgatc
     1621 agctcggcgt cgccgtacat ccgcccgagc aggtgcatgc cgatcggccg gccgtcgggt
     1681 tcaccgctcg ggatgccgag gcccggcagt cccgtgaggt tggcggcgct gagcatccgc
     1741 agggcactgc ccagtgactc gccgtcgccg gcgtccgtca gctcgctggc catcgtggcc
     1801 agcagcggg
//
LOCUS       DATMSX010000484        10271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_159208,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000484 DATMSX010000000
VERSION     DATMSX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159208"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VN041_15355"
     CDS             complement(<1..930)
                     /locus_tag="VN041_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWU30458.1"
                     /translation="METAALVVVLVIALALFFDFTNGFHDTANAMATPIATGALKPKT
                     AVLLAAVLNLVGAFLSTEVSKTVSGGIINESAVGHTLLPELIFAGLIGAITWNMLTWL
                     LGLPSSSSHALFGGLIGATIVGFSASGINFGVVLSKVILPALIAPLTAGIIAYVATKV
                     AYGMTRRYDGKPDGRSGFRWGQIFTSSMVALSHGTNDAQKTMGVITLALITVGWQSGD
                     PGSPEYHNPEFWVIVACAGTIALGTYLGGWRIIRTLGKGLTEVKPAQGFAAESSTAAT
                     ILASSAFGFALSTTQVASGSVIGSGLGRRGSTVR"
     gene            1127..3238
                     /locus_tag="VN041_15360"
     CDS             1127..3238
                     /locus_tag="VN041_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWU30459.1"
                     /translation="MSELSIQPPVADRRPAARTHHGDTFEDPYEWLRAKEDAEVVAHL
                     EAENAYTDARTAHLSGLRDEIFQEVKSRTLETDLSVPTRRGDWWYYGRTEEGAQYGIQ
                     CRTAISGPDDWTPPVLEPGVAVPGEQVLLDGNVEAEGHEFFSMGAFDLSDDGTRMLWS
                     VDFEGDELYTAHVRDLVTGERLADEIPNTGQAFFTPDGLSVVYTTRDEAWRPDTLWLH
                     RLGTPSDADVKLFHEPDERYWLGAGITRSKKYLVIGLGSKITSEELLLDLTDLTTAPQ
                     PVWPRVEGVEYSVDHAVIDGEDRLFILHNDGALDFELVSVAASDPQGERRVVLPHTPG
                     RRLLDVDCFRDFATVEYRTEGMPRAALLDYRTDALDAVEFDEPLYETYFSGNPEWHAP
                     FLRMGYTSFVTPSTVLELELATGEKHVRKQMPVLGGYDSSDYGQQRVWATAPDGAQVP
                     ISLVWKRSFGEPGAEVRPVHLYGYGSYEHSIDPGFSTMRLSMMDRGVIFAVAHVRGGG
                     EMGRHWYEDGKELTKRNTFTDFVAAGRHLVESGVTSPERMVAEGGSAGGLLMGAVTNL
                     APELFAGVLAAVPFVDALTTILDPSLPLTVIEWDEWGDPLHDPEVYAYMKSYTPYENI
                     AADASAYPRILAMTSLNDTRVLYVEPAKWVQALRHAGAADVIMKCEMSAGHGGVSGRY
                     NAWRERAFELAWMLDVLGLAG"
     gene            3757..4530
                     /locus_tag="VN041_15365"
     CDS             3757..4530
                     /locus_tag="VN041_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30460.1"
                     /translation="MLQKRLRAEVDGFAPALTPHQFFSHETGLALLGVPLPYTRDAHR
                     EVHVSTRRPHPLPRRRGVTGHRLQSREPAYWHRESLRIEHPARLWRQAAAHWELDDLI
                     VAGDFLVHPRNRLLTVNDLRQEVAEAGDVRGLLSAALEEIRVGAESPEETRLRLLLTR
                     AGLPEPELNVELFDRRGGFIARLDLAYPEYRVAPEYDGRGHADEGQFAKDADRWDAIR
                     ERGWQHVRILSHHMRPNPQLAVDKVAVALFAAGWRPGRG"
     gene            complement(4690..5718)
                     /locus_tag="VN041_15370"
     CDS             complement(4690..5718)
                     /locus_tag="VN041_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWU30461.1"
                     /translation="MKIGILTSGGDCPGLNAVIRGIVLKGTTTYNLEFVGIRDGWRGV
                     VDGDFFPLTRHEVKGLSKVGGTILGTSRTNPYEGVRGGAENIAKTLAAHGIDGIVAIG
                     GEGTLAAADRLSKDGINVLGVPKTIDNDLRATDYSFGFDTAVNIATDAMDRLRTTGDS
                     HQRCMVAEVMGRHVGWIALHAGMAAGAHVICIPEVPMSIDEICALVTRAFDRGRAPLV
                     VVSEGFKLSGMDEAYSDKGLDAFNRPRLGGISEILAPEIERITGIETRSTVLGHIQRG
                     GSPSGFDRVLATRLGLNAADALVDECWGQMVAMRGTDIVRVPFEEALGELNTVPLSRY
                     EEASALFG"
     gene            complement(5941..8088)
                     /locus_tag="VN041_15375"
     CDS             complement(5941..8088)
                     /locus_tag="VN041_15375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWU30462.1"
                     /translation="MPTTATAASRRKKTSRRDEEAPLIPILARKVREIEAKAQRSKLG
                     PTNRVKFQVIAFLVREERARVKSDAELGDSARAELLKRLDGVATILAKTAARDTSLIQ
                     LLEADQATSPVAKRMRRDWLLESGAELAPEELIITDIAPVRTTQVVPAALAEKQVTPP
                     SVESRQLANPFLAPDLTPRPDSVPRRRLDGWELMGPLYKAFESGAGGSAASMELPPLP
                     EYDHISPKGREVMVHQSRFVESVRAGHRSFLLADEPGLGKTAQSVLAASVAEAYPLLV
                     VVPNVVKMNWAREVELWTPQRRATVIQGDGDDIDAFADVFIVNYEILDRHLSWLGAIG
                     LKGMVVDEAHFIKNLSSQRSQNVLALASRIREREQHPLMMALTGTPLINDVEDFDAIW
                     RFLGWTNGEKPEAPLMEKLDATGLTPADKSFYAEAREAVISMGIVRRKKKDVAADLPD
                     KLIADLPVQLDDEYGRSIRAAERELGERMAAKYRRIVEARAAAHGPSGSHEIDDDIVR
                     LVAHNELEESKAAGTGGDNVFTMVRRIGQAKAMLAADYAAQLQRSVEKVVFFAKHIDV
                     MDQAEAHFAASGIKAVSIRGDQSTPARQQAIDAFNNDPSVGIAVCSLTAAGVGVNLQV
                     ASNVVLAELSWTAAEQTQAIDRVHRIGQDEPVTAWRIIAAHTIDTKIAELIDQKQGLA
                     ARALDGEAVEEAPGESVQLAALMHLLREALGAA"
     gene            complement(8260..9207)
                     /locus_tag="VN041_15380"
     CDS             complement(8260..9207)
                     /locus_tag="VN041_15380"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWU30463.1"
                     /translation="MIVFRDPSEVPADFGPSVVAIGKFDGVHVGHRAIIERMRVDAAA
                     VGAKTVAVTFDRNPLEILRPELCPENVVATDRKVQLLAELDLDATLVLTFDRALASLA
                     AEDFVRSILVDALHVVTVLVGRDFRFGRGGAGTPELLRELGPTYGFTVDVIDDVHPDG
                     EQRRVSSTWIRELLAAGDVVEAAKALGRPVAVTGEVVHGLKRGRELGFPTANLSELID
                     ALAPADGVYAGWLDDHDIGIRHPAAISVGTNPTFDDVERRQVEAHVLDESGLELYGHR
                     VTVEFTDHLRGMVAFEGMDALIAQIAADVAEARTRLGLG"
     gene            complement(9204..9596)
                     /locus_tag="VN041_15385"
     CDS             complement(9204..9596)
                     /locus_tag="VN041_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30464.1"
                     /translation="MTGIGRVLVIVYAVMALGATGRSFVQIVRRFDEAPLAYSLSALA
                     AVVYILATLALVLAHRRGWYAVAWIAILFELTGVVTVGLLSLLVPTLFGHDSVWSYFG
                     MGYVFIPLVLPLFGLWWLRTHRPQEVSA"
     gene            complement(9702..10070)
                     /locus_tag="VN041_15390"
     CDS             complement(9702..10070)
                     /locus_tag="VN041_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30465.1"
                     /translation="MILWFTIVQIAIACASGLFCLIAGLVGRRPSDYTVGSLLLVEVL
                     LIVQVVIAIIAPLAGNPPTGDLLEYWVYLIAAVLLPVGGTAWALLDRTRWSTVVLGVV
                     GLSLAIMLWRMQSIWTLQIG"
     gene            complement(10067..>10271)
                     /locus_tag="VN041_15395"
     CDS             complement(10067..>10271)
                     /locus_tag="VN041_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWU30466.1"
                     /translation="ATRVMPALDVTAEEARDLRHGKRLVGQRDRLDAAEVAAIDPAGF
                     LVGILEARGHDIKSAMNMPEVQA"
BASE COUNT         1597 a         3489 c         3597 g         1588 t
ORIGIN      
        1 gcgcaccgtg gagccgcgac gccccaggcc cgagccgatc accgagccgg aggccacctg
       61 cgtggtggac agcgcgaagc cgaaggcgct ggaggcgagg atcgtggcgg cggtcgagct
      121 ctccgcggcg aagccctgtg cgggcttgac ctcggtcagt cccttgccca gcgtgcggat
      181 gatgcgccat ccgccgaggt acgtgcccag cgcgatggtg ccggcgcacg cgacgatcac
      241 ccagaactcc gggttgtggt actcggggct gcccgggtcg ccgctctgcc atccgaccgt
      301 gatcagggcg agcgtgatca cgcccatggt cttctgggca tcgttggtgc cgtgcgagag
      361 cgcgaccatg gacgatgtga agatctgccc ccaccggaag ccggagcggc cgtcgggctt
      421 gccgtcgtag cggcgcgtca tgccgtaggc gaccttggtc gccacgtacg cgatgatgcc
      481 cgcggtcagc ggcgcgatga gcgccggcag gatgaccttg gagagcacga cgccgaaatt
      541 gatgccgctg gcgctgaagc cgacgatggt ggcgccgatc aggcctccga agagggcgtg
      601 cgaggagctg gacgggagcc ccagcagcca ggtgagcatg ttccacgtga tggcgccgat
      661 caggccggcg aagatcagct ccggcagcag cgtgtggccg accgccgact cgttgatgat
      721 gccgccggag acggtcttgg acacctccgt cgacaggaac gcgccgacga ggttcagcac
      781 cgcggccagc agaacggccg tcttgggctt gagggctccg gtcgcgatcg gcgtcgccat
      841 cgcgttcgcc gtgtcgtgga agccattggt gaaatcgaag aagagtgcca gtgcgatgac
      901 cagcacgacg acgagggctg cggtttccac cgttctcttt cgttgggact gtcaggaggg
      961 gatgccggtg cgccggcgtg acgaacgaag agttcaccgt gggttcatgt tccgtatacg
     1021 ggcccgcgag aatcctccca tcctcccccg cctcggtcaa cttcgcaccc cctcgtgcgt
     1081 catggccggc gcgcccgggg cagggctgct ccgatagcgt ggacccgtga gcgaactcag
     1141 catccaaccg cccgtcgccg accgccgtcc cgccgcccgc acccaccacg gcgacacctt
     1201 cgaggacccg tacgaatggc tgcgcgccaa ggaagacgcc gaggtcgtgg cgcacctcga
     1261 ggcggagaac gcgtacacgg acgcccgcac cgcccacctg agcggacttc gcgacgagat
     1321 cttccaggag gtgaagtcgc gcacgctcga gacggacctc tccgtgccga ctcgccgggg
     1381 cgactggtgg tactacggcc gcaccgagga gggcgcccag tacggcatcc agtgccgaac
     1441 cgcgatctcc ggcccggacg actggactcc cccggtgctc gagccgggcg tcgccgttcc
     1501 cggcgagcag gtgctgctgg acggcaacgt cgaggccgag gggcacgagt tcttctcgat
     1561 gggcgccttc gacctctccg acgacggcac gcggatgctc tggtcggtgg acttcgaggg
     1621 cgacgagctg tacaccgccc acgtgcgcga tctcgtgacg ggcgagcgcc tcgcggacga
     1681 gatccccaac accggtcagg cgttcttcac cccggacggg ctgtccgtcg tctacacgac
     1741 ccgcgatgag gcgtggcggc cggacaccct gtggctgcac cggctcggaa cgccgtcgga
     1801 cgcggatgtg aagctgttcc acgagcccga cgagcgctac tggctgggcg cgggcatcac
     1861 gcgcagcaag aagtacctcg tcatcgggct gggctccaag atcaccagcg aggagctgct
     1921 gctcgacctg accgatctga ccaccgcacc gcagcccgtc tggccccgcg tggaaggcgt
     1981 ggagtactcc gtggatcacg ccgtcatcga cggcgaggac cgcctcttca tccttcacaa
     2041 cgacggcgca ctcgacttcg agctcgtctc ggtcgcggca tccgacccgc agggcgagcg
     2101 gcgcgtggtg ctgccgcaca cccccggccg gcgactgctc gacgtggact gcttccgcga
     2161 cttcgcgacc gtcgagtacc gcaccgaggg catgccgcgt gcggcgctgc tggactaccg
     2221 caccgacgcg ctcgacgccg tggagttcga cgagccgctg tacgagacgt acttctccgg
     2281 gaaccccgag tggcacgcac cgttcctgcg gatgggctac acctcgttcg tgacgccgtc
     2341 gaccgtgctg gagctggagc tcgcgaccgg cgagaagcac gtgcgcaagc agatgccggt
     2401 gctcggcggc tacgactcgt ccgactacgg tcagcagcgt gtgtgggcca ccgcccccga
     2461 cggcgcccag gtgccgatct cgctggtgtg gaagcggtcg ttcggcgaac ccggcgccga
     2521 ggtgcgtccc gtgcacctgt acggctacgg gtcgtacgag cactccatcg accccggctt
     2581 ctcgacgatg cgcctgtcga tgatggaccg cggcgtgatc ttcgccgtcg cgcacgtccg
     2641 cggcggcggc gagatgggcc ggcactggta tgaggacggc aaggagctga ccaagcgcaa
     2701 caccttcacc gatttcgtcg ccgccggccg ccacctggtc gagagcgggg tgaccagccc
     2761 ggagcggatg gtcgccgagg gcggcagcgc gggcgggctg ctgatgggcg cggtcacgaa
     2821 cctcgccccg gagctgttcg ccggcgtcct ggccgccgtg ccgttcgtcg acgcgctgac
     2881 cacgatcctc gacccgtcgc tgccgctcac cgtgatcgag tgggatgagt ggggcgaccc
     2941 gctgcacgat cccgaggtgt acgcgtacat gaagtcgtac acgccgtacg agaacatcgc
     3001 ggccgatgcc tcggcctatc cgcgcatcct cgcgatgacc tcgctcaacg acacccgcgt
     3061 gctgtacgtc gagccggcga agtgggtgca ggcgctgcgc cacgcgggtg ccgccgacgt
     3121 gatcatgaag tgcgagatgt cggccggcca cggcggcgtg agcggccggt acaacgcgtg
     3181 gcgcgaacgc gcgttcgagc tcgcctggat gctggacgtg ctgggcctgg ctggctgaac
     3241 tcctcctcac tcccgttgcc gtggtcggtt ccgcgcgtcc ggatggcgtg ttccggtagt
     3301 ggtcggtgcg ttgagtccgg agccaccagt gctgggacac gccagaggca tgtcccagca
     3361 ccggtgggtg tgggagtctg tcaccgaccg attctgaggc cggagagctg ccctgtcgca
     3421 gaacgggtcg agttcgggcg cagcagttcg acgtgacgcc gagccgttct ccctgcacat
     3481 cgtcggaatc cgaagcggtt ctccactggc cccggctttc ggggcatgtg ccggatgcga
     3541 acgaggttcg atggcgggat gacacgccga aacgaacttc cggattcgtt gcgcagcacg
     3601 cccttcacta ccgcggaagc gcgcgcgatg ggggtcggcc ggtcacgtct gcgggcgaag
     3661 gacctgtcag ctcccacacg cggcgtgcga cgcctgcggg aggtgggggc gtcgacgcag
     3721 gatgacgacg actccacgcc aagcggacgg atgcggatgc tgcagaagcg actgcgtgcc
     3781 gaggtcgacg gcttcgctcc cgcgctcacg cctcaccagt tcttcagcca tgagaccggt
     3841 ctcgctctgc tcggcgttcc gctgccctac acgagggatg cccatcgcga ggtgcacgtg
     3901 tccacccgtc ggccacaccc gctgccacgt cgccgcggcg tcaccggcca tcgtcttcaa
     3961 tcacgcgaac cggcgtactg gcacagggag tcgctgcgga tcgaacatcc ggctcgactc
     4021 tggcggcaag ctgccgccca ctgggagctg gacgatctca tcgtcgccgg cgacttcctc
     4081 gtccacccgc ggaacaggct gctcaccgtg aacgaccttc gtcaggaagt ggccgaggcc
     4141 ggcgatgtgc gcggcctgct gagcgccgcc ctcgaagaga tccgggtcgg cgccgagagc
     4201 cccgaggaga cccggctccg cctgctgctc acacgcgccg gtcttcccga gccggagctc
     4261 aacgtcgagc tgttcgatcg tcgcggcgga ttcatcgccc ggctggacct cgcctacccc
     4321 gagtatcggg tcgcacctga gtatgacggc cgtggccatg cggacgaagg ccagttcgcg
     4381 aaggacgccg accgctggga cgccatccgc gagcggggct ggcagcacgt ccgcatcctc
     4441 agccaccata tgcgcccgaa tccacagctc gccgtagaca aggtcgcagt cgcgctgttc
     4501 gccgcggggt ggcgtccggg acgcgggtga cgtcgagtct cacaagatac gggtggcgcg
     4561 gcatccgacc cgccgttgcg gcgacacggc cagccgcatg tcccagaagc ggcgggtgcg
     4621 ggcgccgtac acccgccata tgtgcgacat gccctggctc cgctcgacgc gcgccggtgt
     4681 cgaagaacct cagccgaaca gcgcggaggc ctcttcgtag cggctgagcg gcacggtgtt
     4741 cagctcgccg agcgcctcct cgaagggcac gcgcacgatg tcggtgccgc gcatcgcgac
     4801 catctgaccc cagcactcgt ccacgagggc gtcggccgcg ttcaggccga ggcgagtcgc
     4861 gagcacgcgg tcgaagcccg acggggaacc gccgcgctgg atgtggccga gcaccgtcga
     4921 gcgggtctcg atgccggtga tccgctcgat ctcgggtgcc aggatctcgc tgatgccgcc
     4981 gagccgcggg cggttgaacg cgtccaggcc cttgtcgctg tacgcctcgt ccatgccgct
     5041 cagcttgaag ccctcggaga cgaccaccag cggcgcgcga ccgcgatcga aggcacgggt
     5101 gacgagcgcg cagatctcat cgatcgacat cggcacctcg gggatgcaga tgacgtgggc
     5161 acccgccgcc atcccggcgt gcagtgcgat ccagccgacg tgccggccca tgacctcggc
     5221 gaccatgcag cgctggtggg agtcgccagt ggtgcgaagc ctgtccatgg cgtcggtggc
     5281 gatgttcacg gcggtgtcga atccgaacga gtagtccgtc gcccgcaggt cgttgtcgat
     5341 cgtcttcggc acgccgagca cgttgatgcc gtccttggag agccggtccg cggcggccag
     5401 cgttccctct ccgccgatcg cgacgatgcc gtcgatgccg tgcgcggcaa gggtcttcgc
     5461 gatgttctcc gctcccccgc gcacgccctc gtaagggttg gtgcgactgg tgccgaggat
     5521 cgtgccgccg accttggaca gacccttcac ctcgtgacgg gtgaggggga agaagtcgcc
     5581 gtcgacgacg ccgcgccagc cgtcacggat gccgacgaac tccaggttgt acgtggtggt
     5641 gcccttgagc acgatgccgc ggatgacggc gttgagtccg gggcagtcgc cgccgctggt
     5701 gaggatgccg atcttcatgc tgctgccttc gtgcttcggg aggagagggg aaccggcgac
     5761 gttgcctgtt ctcgacccta gtggggatgc accgcggatg ccattcctga aggcgcgaaa
     5821 accaagaatc ttgacgtcgg atcccaggca tccgatgagt tcttgacgaa acctgccgat
     5881 atcgacgaaa gagggcgcca agaacgtgtg ttcttaacgc cctcttcggg aatgtgacga
     5941 tcaggcggcg ccgagcgcct cccgcagcag gtgcatgagg gccgcgagct gcacggattc
     6001 gcccggggcc tcctccaccg cctcgccgtc cagggcgcgc gcggcgagcc cctgcttctg
     6061 gtcgatgagc tcggcgatct tcgtgtcgat cgtgtgcgcg gcgatgatcc gccacgcggt
     6121 gaccggctcg tcctgcccga tgcggtgcac ccggtcgatg gcctgggtct gctccgccgc
     6181 ggtccagctg agctcggcca ggacgacgtt cgaggccacc tgcaggttga cgccgacgcc
     6241 cgcagcggtc agcgagcaga ccgcgatgcc caccgagggg tcgttgttga acgcgtcgat
     6301 ggcctgctga cgtgccgggg tggactggtc gccgcggatc gacaccgcct tgatcccgga
     6361 cgccgcgaaa tgcgcctcgg cctggtccat gacgtcgatg tgcttggcga agaagacgac
     6421 cttctccacc gagcgctgaa gctgggcggc gtagtcggcc gcgagcatcg ccttggcctg
     6481 gccgatccgg cgcaccatcg tgaagacgtt gtcgccgccg gtgccggcgg ccttcgactc
     6541 ctcgagctcg ttgtgcgcga ccagacggac gatgtcgtcg tcgatctcgt gcgagccgga
     6601 cggaccgtgg gcggccgcgc gggcctcgac gatgcgtcgg tacttggcgg ccatccgctc
     6661 tcccagctcg cgctcggcgg ctcggatgct gcgcccgtat tcgtcgtcca gctggacggg
     6721 aaggtcggcg atgagcttgt cggggaggtc ggcggcgacg tccttcttct ttcggcgcac
     6781 gatgcccatc gagatgacag cctcgcgcgc ctcggcgtag aaggacttgt cagccggcgt
     6841 gagtccggtg gcgtcgagct tctccatcag aggcgcctcg ggcttctcgc cgttggtcca
     6901 gcccaggaac cgccagatcg cgtcgaagtc ctcgacgtcg ttgatcagcg gggttccggt
     6961 cagcgccatc atcagcggat gctgctcgcg ctcgcggatg cgggaggcca gcgcgagcac
     7021 gttctgagac cgctgcgagg acaggttctt gatgaagtgc gcctcgtcga cgaccatgcc
     7081 cttcaggccg atcgcgccga gccaggacag gtgcctgtcg aggatctcgt agttcacgat
     7141 gaacacgtcg gcgaaggcgt cgatgtcgtc gccgtcgccc tggatgaccg tggcacggcg
     7201 ctgcggcgtc cacagctcca cctcgcgcgc ccagttcatc ttcaccacgt tcgggacgac
     7261 gacgagcagc gggtaggcct cggcgacgga tgccgcgagc accgactgcg cggtcttgcc
     7321 caggcccggc tcatctgcca gcaggaagct gcggtgcccg gcgcgcacgg actccacgaa
     7381 ccgcgactgg tggaccatga cctcgcgccc cttgggcgag atgtggtcgt actcgggcag
     7441 cggcggcagc tccatggagg ccgcgcttcc gcccgcaccg gactcgaacg ccttgtacag
     7501 cgggcccatg agctcccagc cgtccaggcg gcgccgaggc acggagtccg ggcgcggcgt
     7561 gagatcaggg gcgaggaacg ggttcgcgag ctgacgcgac tcgaccgacg gcggggtgac
     7621 ctgcttctca gcgagggcag cgggcacgac ctgcgtggtg cgcaccggcg cgatgtcggt
     7681 gatgatgagc tcctcgggcg cgagctcggc gcccgactcg agcagccagt cgcgacgcat
     7741 ccgcttggcg accggcgagg tcgcctggtc cgcctccagc agctggatca gcgacgtgtc
     7801 gcgtgctgcg gtcttggcga ggatcgtggc cacaccgtcc aggcgcttga gcagctcggc
     7861 gcgcgcggag tcgccgagct cggcgtccga cttcaccctg gcgcgctcct cgcgcacgag
     7921 gaacgcgatc acctggaact tgacgcggtt cgtggggccg agcttggatc gctgggcctt
     7981 cgcctcgatc tcgcggacct tgcgcgcaag gatcgggatc aggggtgcct cttcgtctcg
     8041 acgagaggtc ttcttgcgcc gcgatgcggc ggtcgccgtg gtcggcatgc tcctcctgag
     8101 cgtgaatgcc gaagtgccga agaggccttc ggttcctact gtgttccgcg tgtggcgcca
     8161 gccaggaccg tgcggagtgc ggctctcgga ctgcgcgcgc agcggcggct tcgagtgcca
     8221 ggcgccagtt tacggcatcc ggatgccgaa tgcggggttt cagcccaggc cgagtcgcgt
     8281 ccgcgcctcg gcgacatccg ccgcgatctg cgcgatcagc gcatccatgc cctcgaacgc
     8341 gaccatgccg cgcaggtggt cggtgaactc caccgtgacc cggtggccgt acagttcgag
     8401 gcccgactcg tcgagcacgt gcgcctcgac ctgacgtcgt tcgacgtcgt cgaaggtcgg
     8461 gttggtgccc accgagatcg cggcgggatg ccggatgccg atgtcatgat cgtccagcca
     8521 gccggcgtac acgccgtccg cgggtgcgag cgcgtcgatg agctcggaga ggttcgcggt
     8581 cgggaagccg agctcgcgcc cgcgcttgag cccgtgcacg acctcgccgg tgacggcgac
     8641 cgggcgcccg agcgccttcg ccgcctcgac gacgtcaccc gccgccagca gttcgcggat
     8701 ccacgtcgac gagacgcgac gttgctcgcc gtccggatgc acgtcgtcga tgacgtcgac
     8761 ggtgaagccg tacgtcgggc cgagctcgcg cagcagctcg ggcgtgcccg cacccccgcg
     8821 gccgaagcgg aagtcccgtc cgacgagcac ggtcaccacg tgcagcgcat ccaccaggat
     8881 gctgcgcacg aagtcctccg cggcgagcga cgccagggcc ctgtcgaagg tgagcacgag
     8941 ggtggcgtcg aggtcgagct cggccagcag ttggaccttg cggtcggtgg ccacgacgtt
     9001 ctcggggcac agctcggggc gcaggatctc gagcgggttg cggtcgaagg tcaccgcgac
     9061 cgtcttggca cccacggccg ccgcatcgac ccgcatccgc tcgatgatcg cgcggtggcc
     9121 gacgtgcacg ccgtcgaact tgccgatggc gacgacactg gggccgaagt cggcggggac
     9181 ctcggacggg tctcggaaga cgatcatgcg gagacttcct gcggtcggtg cgtgcgcagc
     9241 caccacagac cgaacagcgg cagcacgaga gggatgaaga cgtagcccat gccgaagtac
     9301 gaccacacgc tgtcgtggcc gaacagcgtc ggcaccagca ggctcagcag gcccacagtg
     9361 accacgccgg tcagctcgaa caggatggcg atccaggcga ccgcgtacca gccgcgccga
     9421 tgcgcgagga caagcgcgag cgtggcaagg atgtacacga ctgcagcgag ggcggagagc
     9481 gagtacgcca gcggggcttc gtcgaagcgc cgcacgatct gcacgaagct gcgcccggtc
     9541 gcgcccagtg ccatgacggc gtagacgatc acgagaaccc gcccgatacc cgtcatccgg
     9601 gtgcgcgcgg ggggctgggg cggagcatcg gtgtgggaca tcgcatccac catcctatgc
     9661 cgccgcgggc agcacgagcc gcggcgggtc gcagcgggcg gtcatccgat ctgcagggtc
     9721 cagatgctct gcatccgcca cagcatgatc gccagcgaca ggccgaccac gccgagcacg
     9781 accgtgctcc agcgggtgcg gtcaagcagc gcccaggcgg tgccgccgac aggaagcagg
     9841 acggcggcga tcaggtacac ccagtactcg agcaggtccc cggtgggcgg attgccggcc
     9901 agcggcgcga tgatcgcgat cacgacctgc acgatcagca gcacctcgac cagcagcagg
     9961 ctgcccacgg tgtagtcgct gggtcgacgt ccgacgaggc cggcgatcag gcagaagagc
    10021 ccggatgcgc aggcgatcgc gatctgcacg atcgtgaacc acaggatcac gcctgcacct
    10081 ccggcatgtt catggcgctc ttgatgtcgt gcccgcgggc ctcgaggatg ccgacgagaa
    10141 atccggccgg gtcgatcgcc gccacctcgg cggcatcgag ccggtcgcgc tgcccgacga
    10201 gtcgcttgcc gtggcggagg tctcgcgcct cctccgcagt gacatcgagc gcgggcatga
    10261 cgcgggtcgc g
//
LOCUS       DATMSX010000485         4263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_130904,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000485 DATMSX010000000
VERSION     DATMSX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130904"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VN041_15400"
     CDS             complement(<1..529)
                     /locus_tag="VN041_15400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30467.1"
                     /translation="MGALSLEGLVAIVTGGASGIGAAVARRLHGEGAQIAVLDRDTSG
                     ADAAFAAFTADVSDRASVDAAVAAVAERFGRIDIVINNAGIGAQGDVTANDDDEWARV
                     LSINVTGIARVTSAALPWLRKSPSAAVCNTASIASTTGLPQRALYSASKGAVSALTRA
                     MAADHLREGIRDSETS"
     gene            complement(517..1371)
                     /locus_tag="VN041_15405"
     CDS             complement(517..1371)
                     /locus_tag="VN041_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU30468.1"
                     /translation="MKFARLGEKGSEIPVVIDEGRTFDLRPVTSDVNGEFLASDPVGR
                     TRAALDAGTLPELADADALRIGAPIARPSAVICIGMNYAAHAAESGSEPPTIPILFLK
                     TPNTVVGPNDAVTIPRGSEKTDWEVELGVVIGTRTAYLDSPEEADAHIAGYVLANDVS
                     ERDFQMAVSGGQWSKGKIAAGFNPTGPWLVPASEVDSTNLRLRSWVNGEPRQDSNTSD
                     LIFDVRTIVHHLSQYVTLEPGDLILTGTPQGVALSGRFPYLSPGDVVEVEIDGLGRQK
                     QEFVAWAR"
     gene            1494..2786
                     /locus_tag="VN041_15410"
     CDS             1494..2786
                     /locus_tag="VN041_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HWU30469.1"
                     /translation="MSRIVALETSDIRFPTSLSLDGSDAMNPDPDYSAAYVIVRTDAA
                     DDIAGHAFVFTIGRGNDVQVAAIDALAGHLVGREIEPLLDDMGGTFRDIIGDSQLRWL
                     GPEKGVMHMAIGAVINALWDIKAKRAGLPLWQLLGRMSPEELVDLVDFRYLTNALTRD
                     EALEILRAAVPGREEREAQLLASGYPGYTTSPGWLGYSDEKLERLAREAMADGFTQIK
                     LKVGADLQDDIRRFRKAREVCGPDFPIAIDANQRWEVSEAIEWVNALAEFHPAWIEEP
                     TSPDDILGHAEIARGVAPIRVATGEHAQNRMMFKQLLQADAIKIMQIDAVRVAGVNEN
                     IANLLLAAKFGVPVCPHAGGVGLCEAVQHLSMFDFVAVSGSREGRLIEYVDHLHEHFV
                     VPTDIQGGSYMAPTAPGTSMEMKADSIAKYTWTGQHVA"
     gene            2776..3732
                     /locus_tag="VN041_15415"
     CDS             2776..3732
                     /locus_tag="VN041_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU30470.1"
                     /translation="MSLELPALGYGAANVGNLFRALSDDEAWAVLEAAWDAGIRYFDT
                     APHYGLGLSERRLGAFLQTKPRDDFVISTKAGRLLRPNPAHQAGGLDTDNDFHVPDDL
                     RREWDFSEAGIRTSIAESQERLGLDRIDLLYLHDPERHDLDLALAEAFPALEKARSEG
                     LIGAIGIGSMVSDALSRAVREADLDLIMVAGRYTLLEQPAAADVLPACAETGTGIVAA
                     SVFNSGLLAQSEPRRDGRYEYGQLPEELWDRLVRIASICRAHDVPLPAAAIQFPLQSD
                     LVRSVVVGGSRPAQLRQNAEYAALEIPADLWAELAEDGLIPA"
     gene            3775..4194
                     /locus_tag="VN041_15420"
     CDS             3775..4194
                     /locus_tag="VN041_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HWU30471.1"
                     /translation="MLEGINPLLTGELLLHLDRMGHSDSVVVADAHFPAWALGERVID
                     LPGTTTPEVVAAIRSVLPLDDAPGMDLMESADAEVLDVQHELMAAAGTALDTTRFVER
                     FAYYDVAQGAYLMVRTGETRKYGNALLRKGVVGHPSA"
BASE COUNT          640 a         1469 c         1516 g          638 t
ORIGIN      
        1 tgctggtctc gctgtcgcgg atgccctcgc gcaggtggtc cgcggccatc gcccgggtca
       61 gtgcggagac cgcgcccttc gacgcggagt acagcgcacg ctgcggcagg ccggtcgtgg
      121 aggcgatgga tgccgtgttg cagaccgccg ccgagggcga cttccgcagc cagggcaggg
      181 ccgccgaggt cacgcgggcg atgccggtga cgttgatgga gagcacgcgg gcccactcgt
      241 cgtcgtcgtt cgcggtcaca tcgccctgtg cgccgatgcc ggcgttgttg atcacgatgt
      301 cgatgcgtcc gaaccgttcc gcgaccgccg cgacggcggc gtcgacggac gcgcggtcgg
      361 agacgtcggc cgtgaacgcc gcgaatgcgg catccgctcc ggacgtgtcg cggtcgagga
      421 cggcgatctg cgcgccctcc ccgtgcaggc ggcgggccac cgccgcgccg attccggatg
      481 ctccgccggt gacgatcgcc accaggcctt cgagactcag cgcgcccatg ccacgaactc
      541 ctgcttctga cggcccaggc cgtcgatctc gacctcgacg acatcgccgg ggctcaggta
      601 ggggaagcgc cccgacaggg cgacgccctg cggggtgccg gtgaggatca ggtcgcccgg
      661 ctcgagcgtg acgtactgcg acaggtggtg cacgatcgtg cgcacgtcga agatcaggtc
      721 gctggtgttc gagtcctggc gcggctcccc gttgacccag ctgcgcaggc ggaggttcgt
      781 cgagtcgacc tcggatgccg gcaccagcca ggggccggtg gggttgaagc ctgcggcgat
      841 cttgcccttg gaccactgcc cgccggacac ggccatctgg aagtcgcgct ccgacacgtc
      901 gttcgcgagc acgtagccgg cgatgtgggc atccgcctcc tcgggcgagt ccaggtaggc
      961 ggtgcgggtg ccgatcacga cgcccagctc gacctcccag tcggtcttct cgctgccgcg
     1021 cggaatggtg acggcgtcgt tggggccgac cacggtgttc ggcgtcttca ggaacaggat
     1081 cgggatggtg ggcggctcgg aacccgactc cgcggcgtgc gcggcgtagt tcatgccgat
     1141 gcagatcacc gcgctcggtc gtgcgatggg ggcgccgatg cgcagggcgt ccgcgtccgc
     1201 cagctcggga agagtgcccg cgtccagcgc ggctcgcgtc cgtccgacgg gatcggatgc
     1261 caggaattcg ccgttcacat cggatgtcac cggacgcaga tcgaaggtgc gaccctcgtc
     1321 gatgacgacg gggatctcgc ttcctttctc gccgagtcgc gcgaacttca tggttctcct
     1381 tctcctggat cgacagcggg tgctgacgcg accccgccgt tgacagtata gacatcggat
     1441 gtttacactc caagagtccg cgtcatattc gtccgacgcg ccgaaggagc cccgtgagcc
     1501 gtatcgtcgc gctggagacc agcgacatcc gcttccccac ctcgctgagc ctggacggct
     1561 cggatgcgat gaaccccgac cccgactact cggcggcgta cgtcatcgtg cgcaccgacg
     1621 ccgcggacga catcgcgggc cacgcgttcg tgttcacgat cggccgcggc aacgacgtgc
     1681 aggtcgccgc gatcgacgca ctcgccggac acctcgtcgg ccgcgagatc gagccgctgc
     1741 tggatgacat gggcggcacc ttccgcgaca tcatcggcga ctcgcagctg cgctggctcg
     1801 ggcccgagaa gggcgtgatg cacatggcca tcggcgccgt gatcaacgcc ctgtgggaca
     1861 tcaaggccaa gcgcgcgggc ctgcccctct ggcagctgct cggccgcatg agccccgagg
     1921 agctcgtcga cctggtcgac ttccgctacc tgaccaacgc gctcacccgc gacgaggcgc
     1981 tcgagatcct ccgcgccgcg gtgccggggc gtgaggagcg cgaggcccag ctgctggcat
     2041 ccggctaccc gggctacacc accagccccg gctggctggg ctactccgac gagaagcttg
     2101 agcgcctcgc ccgcgaggcc atggccgacg gcttcacgca gatcaagctc aaggtcggcg
     2161 ccgacctgca ggacgacatc cgccgcttcc gcaaggcgcg cgaggtgtgc ggacccgact
     2221 tcccgatcgc gatcgatgcc aaccagcgct gggaggtgtc cgaggcgatc gagtgggtca
     2281 acgcgctcgc cgagttccac cccgcctgga tcgaggagcc caccagcccc gacgacatcc
     2341 tcggccacgc cgagatcgcc cgcggcgtcg ccccgatccg cgtggccacc ggcgagcacg
     2401 cccagaaccg gatgatgttc aagcagctgc tgcaggccga cgcgatcaag atcatgcaga
     2461 tcgacgccgt gcgggtcgcg ggcgtgaacg agaacatcgc gaacctcctg ctcgccgcga
     2521 agttcggcgt gccggtgtgc ccgcacgccg gaggcgtggg cctgtgcgag gccgtgcagc
     2581 acctgtcgat gttcgacttc gtcgccgtca gcggctctcg cgagggccgg ctgatcgagt
     2641 acgtcgacca cctgcacgag cacttcgtgg tgccgaccga catccagggc ggctcgtaca
     2701 tggcgcccac cgcaccgggc acgagcatgg agatgaaggc cgacagcatc gcgaagtaca
     2761 cctggacggg gcagcatgtc gcttgagctt cccgcgctcg gctacggcgc cgcgaacgtc
     2821 ggcaacctct tccgcgcgct gagcgacgac gaggcctggg cggttctcga ggccgcctgg
     2881 gatgccggca tccgctactt cgacaccgcc ccgcactacg ggctcgggct gagcgagcgg
     2941 cgtctgggcg cgttcctgca gaccaagcca cgcgacgact tcgtgatttc gaccaaggca
     3001 gggcgcctgc tgcggccgaa cccggcgcat caggcgggcg gactcgacac ggacaacgac
     3061 ttccacgtgc cggacgatct gcgccgcgag tgggacttca gcgaggcagg cattcgtacg
     3121 agcatcgccg agtcgcagga gcggctcgga ctcgaccgga tcgacctgct gtacctgcac
     3181 gaccccgagc gccacgacct cgacctcgcg ctcgccgagg cgttccccgc gctggagaag
     3241 gcgcggtccg aggggctgat cggagcgatc ggcatcgggt cgatggtgtc ggatgccctg
     3301 tcgcgcgccg tgcgcgaggc cgacctcgac ctgatcatgg tcgcgggacg gtacacgctg
     3361 ctggagcagc ccgccgcggc cgacgtgctg ccggcgtgcg cggagaccgg gacggggatc
     3421 gtcgcggcat ccgtgttcaa ctccggcctg ctcgcgcaga gcgagcccag gcgcgacggc
     3481 cggtacgagt acggccagct tcccgaggaa ctgtgggacc gactggtgcg gatcgcgtcg
     3541 atctgcaggg cgcacgacgt gccgcttcct gcggccgcca tccagttccc gctgcagtcg
     3601 gacctcgtac gctcggtcgt ggtcggcggc agccggcccg cgcagctgcg gcagaatgcc
     3661 gagtacgccg cgctggagat ccccgccgat ctctgggcgg agctggccga ggacgggctg
     3721 atcccggcgt gacgccgttc ggatcgtccg cgagagggag aagaggactg acggatgctt
     3781 gaggggatca acccgctgct gaccggtgag ctgctgctgc acctggatcg gatggggcac
     3841 tcggactcgg tcgtcgtggc cgatgcgcat ttcccggcgt gggcgctggg cgagcgggtg
     3901 atcgacctgc cgggcacgac gacgcccgag gtggtcgccg cgatccgctc ggtgctgccg
     3961 ctggacgatg cgcccggcat ggacctgatg gagtcggcgg atgccgaggt gctggacgtg
     4021 cagcacgagc tgatggccgc cgccgggacg gcgctggaca cgacccggtt cgtggagcgc
     4081 ttcgcctact acgacgtcgc ccagggggcg tacctgatgg tgcgcaccgg cgagacccgc
     4141 aagtacggca acgccctgct gcgcaagggc gtcgtggggc acccctcggc ctgaacgttg
     4201 ccgggcggcg gtcagccgac cgtcgaggct cggacgacca gctcggcggc gaagggcgtg
     4261 acc
//
LOCUS       DATMSX010000486        12698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_16657,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000486 DATMSX010000000
VERSION     DATMSX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16657"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VN041_15425"
     CDS             complement(<1..415)
                     /locus_tag="VN041_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30472.1"
                     /translation="MPKTVTPRWRTRLLRADPEAGWEDHAWTSAVLEPGFAADQAVAS
                     WNVDAGVTAHVEIRARGVDGSWSPWLPLADWGEPGARRSPEPTSGDRAEASPFVDTDV
                     LMAAPRHPFDAAQLRITVDGRSGGDARLHLAAVSFT"
     gene            747..2600
                     /locus_tag="VN041_15430"
     CDS             747..2600
                     /locus_tag="VN041_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30473.1"
                     /translation="MRTRHRKHLVRALCVAAAAAMVVAPAAAANAAAPTGIHAAQSSA
                     QPSDKSDAKTLRVATSGFVDSFNPFTSIYLLPTNILRYTYEFLVQNSQEDGSPTKGLA
                     EKWEPSDGGKVWTFTLQDGLKWSDGKPITSADVAYTYQAMMDDPAMGVANGGLLSNLD
                     SIETPDEKTVVLNMKEPQAPNPGVEIPVVPKHVWEKIDKPAEYANDKDVVGSGPFLLE
                     SYKANQSIVLKANKDFWRGAPKIDRIQYIYYTNSDAQVQALRSGDVDMVTGLTATQFE
                     ALDGVKGIATHSGQGRRYSAISFNVGLKTRDGQAYGNGSPALQDETVRQAIRLGTDTD
                     TLLEKVLGGYGEPATSFIPASYPKWTLPADDKVIMKFDPEAAKQKLEEAGWKAGADGI
                     REKDGKKLELRLYVDSTSATEQAEAEFFVPWMKDIGVAIKVESTDTDTITAETTKGNY
                     DMYFSGWSQGADPDYQLGINTCANMPTATDGSGGTSQDGWCDPEFDKLYQEQHTELDQ
                     SKREAIVRKMLEMNYTATPQIATWYANSLEAYRSDRFTDFSLMPKKDGIIANQSGYWG
                     YLTVRPAGDDTAATDGGPNVGLIVTGVVIAVLVIGGIVFVAMRRRNSADIE"
     gene            2681..3721
                     /locus_tag="VN041_15435"
     CDS             2681..3721
                     /locus_tag="VN041_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30474.1"
                     /translation="MSNAVPATEAVILEEQGEVRPRGVPALKYASSKIGGALISLVMV
                     ILLGFFAFKILPGDPARAAARDRQMTPEQLEQLRHQMGLDKPLWQQFTDYLGNVFTLN
                     FGQSYVYKQSVSSLIGEYFWNTILLTGTAAVIAIALGLWLGQKAGWLHGSTFDKLTTG
                     TSLVLWSVPTFWLGLLLLMIFGGTLHWFPTGGMTSPNPPADAPGLVLDVISHMVLPVI
                     TMVAVVYAQYVLVMRASVMEERTADYLVTARAKGLRDDLVRSRHAVPNALLPAVTLVF
                     MHLGGLIAGAVTVETVFSWPGLGKLTFQAIQGPDLPLLQGTFVVFSAIIIVMNLAADF
                     VYRWLDPRVRRA"
     gene            3718..4635
                     /locus_tag="VN041_15440"
     CDS             3718..4635
                     /locus_tag="VN041_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30475.1"
                     /translation="MSAQIATIARSPRRLAWTRRRKALGSFCRQFSHNRTGMAGLILL
                     MLVIIIATFAPVIAPAWMLDATKLIDAPRFAPPSWEHPLGTDHLGREIWVRMVWGAQV
                     SLVVGFAATAVSMLIGTIVGIAAGHFTGWTGGLLMRIIDFFLVLPSLILAIVLSSVLQ
                     RGVWTIVIAIGLTSWAGTARVVRSQTLSVEARDYIERSRVLGAGHWHIIVKHLLPGVL
                     PLVLANTTLTVGSAIIAESTLSFLGLGDTTMQSWGAVLKNSMDYSAATAGYWWYVLVP
                     GIAIVLVVLAFTLVGRAVENIVNPTLRSR"
     gene            4639..6288
                     /locus_tag="VN041_15445"
     CDS             4639..6288
                     /locus_tag="VN041_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30476.1"
                     /translation="MPDLTFDDVSITYRASSSGDGRGEVHAVKNISLTLPAGGTLGVA
                     GESGSGKSTLAMSVLRLLPANAKLTGRVLVGDTDITQLNFGALRALRWADASIIFQGA
                     MHSLNPVRTVGQQILEALELHVTDSWRTDKERRARVAELLRVVDLAPQKIDAYPHEMS
                     GGQKQRVMIAMALACEPDIIIADEPTTALDVIVQKQILEMISELVTDRGISMLMISHD
                     LSVLATACDRIAVMRDGELLEVGESLQVCLEPEHPYTRQLADAFPTIGDPASRLNPVT
                     RRERPAHEREQGMTDEVLLEAKGLNVTYHGRGSALRAVKNVDLEVHRGEILALVGQSG
                     SGKSTLARTLVGLQRPDDGATVRFRGRDLPRKGRALRAFRSEVQMVLQDPTAALNPKL
                     SIYESVAEGLRVQHYPGDEAERVVQSLIDAELTPPERFMAAIPQELSGGQRQRAVIAG
                     ALALGPQMLVADEPVASLDASVRGEILALLLSLRDRLGLSALVITHDLGLAWNIADTV
                     AVMLEGEIVEYGTTEQVLLDPQHEYTRKLLAAAPTIERSGA"
     gene            6285..7271
                     /locus_tag="VN041_15450"
     CDS             6285..7271
                     /locus_tag="VN041_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWU30477.1"
                     /translation="MSGTAVAVDRHAYLTEAPLQPGDRVHFITPSGLGTAESLERAVG
                     YYRDWGLEVTIGEHVLEPHPRAKYLAGPDAGRRGDLVDAWLDPDTAAVVCVRGGYGAM
                     RLLDGIDWELLRGAALRRDGRPKLLTGSSDITALHEAFRVHLDVPTLFCPMPGNDVFR
                     DSAAIRDDVHRWLFEPWGGRGIVGPAAEVLVPGRAAGRFTGGNLALLAGGLGASESAV
                     APEGILFLEDIDEELYRLDGFLLALLRAGRIAAASGIVLGSWHNCAVADGVAQLDGVK
                     ALMTEYLAGLGKPVLWEQGFGHDPNALSVPLNVDGVLEAGDAGIRLAVVEGA"
     gene            7271..8110
                     /locus_tag="VN041_15455"
     CDS             7271..8110
                     /locus_tag="VN041_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWU30478.1"
                     /translation="MSVELPSLDERVSWSIRIVDAGTGELLAEHAPHTQCETASIGKI
                     FLLIEVARRLAEGSLSADQIVPIPAEHVVEDSGLLYRMLKQSLCVHDAALLVGAFSDN
                     LATNALIALCGLEEVRAVAPSLGYADTSLHDYIRSERTPDLPWTPSYGTGAELADVMR
                     RLGAGDVISPTVSGQVMTWLAANADTSMVADSLLLDPLAHVDAEYQDMVLRHKTGSTS
                     FARVDVGHLTGPAGALAYAVAANWKGSAEDLRAPVTDAMRAIGEQLRRHVTGRDRADA
                     TMD"
     gene            8153..8851
                     /locus_tag="VN041_15460"
     CDS             8153..8851
                     /locus_tag="VN041_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HWU30479.1"
                     /translation="MTRNGRLGIGIIGAGKVGPVVGAALAGAGHAITGITSGSDDDRA
                     AAVLPGVPVLDALEVVRRSELVVIAVPRDELPGLVAGIAELGGWQMGQLVLHTDPSYG
                     IEVLRPAAERGAIPLAVHPAIAFTGTTMDLRQLQAAFAAVTAPAPVLPIAQALAVEMG
                     CEPVVIAEQDRAAYGEAIATASEFSRSIVGQATSLLRGVGVENPGGYLSALVSSTVEQ
                     ALREASGPLEPPLP"
     gene            complement(8866..10680)
                     /locus_tag="VN041_15465"
     CDS             complement(8866..10680)
                     /locus_tag="VN041_15465"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWU30480.1"
                     /translation="MRPHLLRPRPHAPRRILVVLALAVGTIAAALLPATAASADVDDF
                     SYSSWVSDYRVSLDADGHAVAHVTETVVAEFPQTDQNKGIIRGYPERYEGAGLSLRIL
                     SVKDAEGRDVPFETESEDGMLLVLTGKDDYVHGSNTYVIESTMRDFMIHGSKSRNDEF
                     YWNLLPLNSTQAIGRFRATVTFAPPLADALTGDTACYSGTTGQKGACTLDGPASSAAG
                     ASFSVESGERAAGDGMTIAIGFDGGTVTQPEARMPDPVADFGPAVVGLGAMATASGAW
                     FSFAALKRRRQTATGIIVAQFDVPAELPPLAAAPLIPGAPNPIPAQMVHLAVQGVLRL
                     EEEQDAKRRPSLRLIDRSRVSQPLDRAAVDSLFLGEETVRRIPKSSTKFAKRMEKLVQ
                     KGRAAAKQNGWLTEERSRAAMAFGWASIALLAATAVFLTWSAMKDRELLGLSIVALIG
                     AGVVVAISSIVAFSKHTVMTYEGALRHEHLMGVKEFIRVAEADRLRMLQSYQGAERRS
                     DGTVDVVHLYEKLLPYAMLLGEEKSWTKVLETGYSASGSGPGWVDVAAGVSLHSHLAN
                     YSTSMNSAATYTASSSSGGSTGGGFSGGGGGGGFSGGR"
     gene            10775..12295
                     /gene="lysS"
                     /locus_tag="VN041_15470"
     CDS             10775..12295
                     /gene="lysS"
                     /locus_tag="VN041_15470"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWU30481.1"
                     /translation="MTDASAPIPSPEFEEDVFEQKAVRLAKRDKLIANRADAGGGAFP
                     VGVPVTHGIPALRAQYGDLEPGAETGEIVGVAGRVVFSRNTGKLCFATLQAGDGSRIQ
                     AMVSLANVGEESLAGWKELVDLGDHVFVHGEVISSRRGELSIMADDWAIASKAILPLP
                     NVYGELNEETRVRSRFLDLIVRDQARTTVRARAAVNASLRATFTSHDYLEVETPMLQV
                     QHGGASARPFITHSNAFDTELYLRIAPELYLKRAVVGGIERVYEINRNFRNEGADSTH
                     SPEFAMLEAYQAYGDYNQMAELTQELVQNAAIAVAGSTTVTWADGTEYDLGGEWDRIS
                     MYDSLSEVSGRTVTPQDSVEDLIAFANENGVEIPPHATHGKLVEELWEHFVKGDLVRP
                     TFVMDFPVDTSPLVREHRSIAGVVEKWDLYVRGFELATGYSELIDPVIQRERFVEQAK
                     LAAGGDLEAMRVDEEFLRALEHGMPPSGGMGMGIDRLLMAVTGLGIRETILFPLVK"
     gene            12311..>12698
                     /locus_tag="VN041_15475"
     CDS             12311..>12698
                     /locus_tag="VN041_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWU30482.1"
                     /translation="MFGIVDLAIAVLLLAAVVAGFGAGLFSILGALCGLVAGALAVPF
                     VLPLIARALPESEWQGLIIVAAAVVLLAVGMGIGSAIGALLRRGADKLRLKFLERMLG
                     GVAALVAGALAITMAGSAIASAGIPGL"
BASE COUNT         1985 a         4285 c         4409 g         2019 t
ORIGIN      
        1 cggtgaagct cacggcggcg agatgcaggc gcgcatcgcc gccggatcgg ccgtcgacag
       61 tgatgcgcag ctgcgcagcg tcgaacggat gccgcggtgc ggccatcagc acgtccgtgt
      121 ccacgaacgg cgaagcctcc gccctgtcgc ccgaggtggg ctcggggctg cgacgcgcac
      181 cgggctcacc ccagtcggcg aggggaagcc acggcgacca gctcccgtcg acaccacggg
      241 cgcggatctc gacgtgcgcc gtgaccccgg catccacgtt ccacgacgcg actgcctgat
      301 cggcggcgaa gccaggctcc agtacggcgg aggtccaggc gtggtcttcc catcctgcct
      361 ccggatcggc gcgcagcagt cgggtgcgcc acctcggcgt cacggtcttc ggcacggtgg
      421 ctcctcgttt gttaggaccg atgatctgtt acggatcatc ggtcgcatcc aggggtgctg
      481 tcaaggctgg cgccagtcat cccagtcacg cgaaggcggg tcttcccagg gtgattccca
      541 gagcatgaca cgtgtcgtag gctgtacgcg cgtcggtgtc gccaccgaga ccctgttcgc
      601 cagatgtcgg ggaagacatc gcgtgagacc cttcgatggc aacgatctgg tacgaatctg
      661 ttgaccagag tgcctgacat gcggttctat aactgaatcc cacgggaccg tctccgcacg
      721 gctcccgcac cgagagaagg atgctgatgc gaactcgtca ccgtaaacat ctggtccgcg
      781 ccctgtgcgt ggccgcggcc gccgcgatgg tcgtcgctcc tgcagcggcc gcgaatgccg
      841 ccgcccctac tggcatccac gcagcccagt cgtcggcaca gccctctgac aagtccgacg
      901 cgaagacgct tcgggtggcg acctcgggct tcgtggactc gttcaacccg ttcacctcga
      961 tctacctgct gccgaccaac atcctgcgct acacgtacga gttcctcgtg cagaactctc
     1021 aggaggacgg ctcgcccacc aagggcctgg cggagaagtg ggagccctcc gacggcggaa
     1081 aggtctggac cttcacactg caggatgggc tgaaatggtc cgacggcaag ccgatcacct
     1141 cggccgacgt cgcctacacc taccaggcga tgatggacga tccggcgatg ggcgtggcca
     1201 acggcggtct gctcagcaac ctcgattcga tcgagacgcc ggacgagaag accgtcgtgc
     1261 tgaacatgaa ggagccgcag gcgccgaacc ccggtgtcga gatcccggtg gtccccaagc
     1321 acgtgtggga gaagatcgac aagcccgccg agtacgcgaa cgacaaggat gtcgtgggat
     1381 cagggccgtt cctgctggag agctacaagg cgaaccagtc catcgtcctg aaggcgaaca
     1441 aggacttctg gaggggtgct ccgaagatcg atcggatcca gtacatctat tacacgaact
     1501 ccgacgccca ggtgcaggcg ctgcgctccg gcgacgtcga catggtcacc ggtctcaccg
     1561 ccacgcagtt cgaggcgctg gacggcgtga agggcatcgc cacgcactcc ggtcagggcc
     1621 ggcgctattc ggcgatcagc ttcaacgtcg ggctgaagac ccgcgacggg caggcgtacg
     1681 gcaacggcag ccccgcactg caggacgaga ccgtgcgaca ggcgatccgc ctcggcaccg
     1741 acaccgacac gctgctcgag aaggtgctcg gcggctacgg cgagccggcc accagcttca
     1801 tcccggcctc ctatccgaag tggacgctgc cggccgatga caaggtcatc atgaagttcg
     1861 atcccgaggc ggcgaagcag aagctggagg aggcaggctg gaaggccggt gccgacggca
     1921 tccgcgagaa ggacggcaag aagctcgagt tgcgcctgta cgtggactcc accagcgcca
     1981 ccgaacaggc cgaggccgag ttcttcgtgc cgtggatgaa ggacatcggc gtcgcgatca
     2041 aggtggagtc gaccgacacc gacacgatca cggccgagac caccaagggc aactatgaca
     2101 tgtacttcag tggctggagc cagggggcgg accccgacta ccagctcggc atcaacacgt
     2161 gcgcgaacat gcccacggcg accgacggtt ccggcggcac ctcgcaggac ggctggtgcg
     2221 acccggagtt cgacaagctg taccaggagc agcacaccga gctcgaccag agcaagcgcg
     2281 aggcgatcgt gcgcaagatg ctcgagatga actacacggc gactccgcag atcgcgacct
     2341 ggtacgccaa cagcctcgag gcctaccgct ccgaccggtt cacggacttc tcgctcatgc
     2401 cgaagaagga cggcatcatc gccaaccagt cgggctactg gggctacctg acggtgcggc
     2461 cggcaggtga cgacaccgcc gcgacggatg gcgggccgaa cgtgggtctg atcgtcacgg
     2521 gagtcgtgat cgccgtcctc gtgatcggcg gcatcgtctt cgtcgcgatg cgacgccgca
     2581 acagcgccga catcgaatga cccgatgatc gtcgtggcgg gagtccgcag ggctcccgcc
     2641 acgacgtctg ttcctgacgg agaaggacaa ggagacgcag atgtcgaatg cggtgccggc
     2701 cacagaggcc gtcatcctgg aggagcaggg tgaggtccgg cctcgcggag tccctgcact
     2761 gaagtacgcg tcgagcaaga tcggcggagc gctgatcagc ctcgtgatgg tgatactgct
     2821 cggcttcttc gcgttcaaga tcctccccgg cgacccggcc cgcgcggcgg cccgagaccg
     2881 ccagatgaca ccggagcagc tcgagcagct gcgccaccag atgggactcg acaagccgct
     2941 ctggcagcag ttcaccgact acctcggcaa cgtgttcacg ctgaacttcg gtcagagcta
     3001 cgtgtacaag cagtcggtgt cctctctcat cggcgagtac ttctggaaca cgatcctgct
     3061 caccggcacc gccgcggtca tcgccatcgc gctcggtctc tggctcgggc agaaggctgg
     3121 atggctgcac ggatcgacct tcgacaagct gaccaccggc acgtcactgg tgctgtggtc
     3181 ggtgccgacg ttctggctcg ggcttctgct gctgatgatc ttcggcggca cgctgcactg
     3241 gttccccacc ggcgggatga cctcgccgaa tccccccgcc gatgcacctg ggctggtcct
     3301 cgacgtcatc tcgcacatgg tgctgccggt gatcaccatg gtcgcggtcg tgtacgccca
     3361 gtacgttctg gtgatgcggg catccgtcat ggaggagagg acggccgact acctggtcac
     3421 ggcgcgggcg aagggcctgc gcgacgatct cgtgcgcagt cggcacgccg tgccgaacgc
     3481 cctgctgccc gcggtcacgc tggtgttcat gcacctcggc gggctcatcg cgggcgcggt
     3541 gaccgtcgag acggtgttct cctggcccgg cctcggcaag ctcaccttcc aggcgatcca
     3601 gggccctgat ctgccgctgc tgcagggcac cttcgtcgtc ttctcggcga tcatcatcgt
     3661 gatgaacctc gccgccgact tcgtctaccg ctggctcgac ccgagagtga ggcgcgcatg
     3721 agtgctcaga tcgccaccat cgcgcgctcg ccgcgcagac tggcatggac gcggcgtcgc
     3781 aaggcgctcg gttccttctg caggcagttc tcgcacaacc gcacgggcat ggcagggctg
     3841 atcctcctga tgctcgtgat catcatcgcg acgttcgcgc cggtcatcgc cccggcatgg
     3901 atgctggatg ccacgaagct gatcgatgcg cctcgcttcg cccctccgtc ctgggagcac
     3961 ccgctgggca cggaccacct gggccgtgag atctgggtgc gcatggtgtg gggcgcgcag
     4021 gtgtcgctgg tcgtcggctt cgccgccacc gccgtgtcga tgctcatcgg cacgatcgtg
     4081 gggatcgccg ccggtcactt caccggctgg accggtggtc tgctgatgcg catcatcgac
     4141 ttcttcctcg tgctgccttc gctgatcctc gcgatcgtgc tgtcgtcggt gctgcagcgc
     4201 ggcgtgtgga cgatcgtcat cgcgatcggt ctcacctcat gggcgggcac ggcccgggtg
     4261 gtgcgctcgc agacgctgtc ggtggaggcg cgcgattaca tcgagcgctc ccgggtgctc
     4321 ggcgccgggc actggcacat catcgtcaag cacctgctgc cgggggtgct gccgctcgtg
     4381 ctcgcgaaca ccacgctcac tgtcgggtcg gccatcatcg ccgagtcgac gttgtcgttt
     4441 ctcggactcg gcgacaccac catgcagtcc tggggtgcgg tgctgaagaa ctcgatggac
     4501 tactccgcag cgaccgccgg ctactggtgg tacgtgctcg tgcccggcat cgcgatcgtg
     4561 ctcgtggtgc tcgccttcac tctggtgggc cgcgccgtgg agaacatcgt caaccccaca
     4621 ttgaggagcc gttgagagat gccggacctg actttcgacg acgtctcgat cacgtaccgc
     4681 gcctcgagca gcggtgacgg ccgcggtgag gtgcatgccg tcaagaacat ctcactcacc
     4741 ctgcccgccg gcggcacgct cggcgtggca ggtgaatccg gctcgggcaa gtcgacgctc
     4801 gcgatgagcg tgctgcgtct gctgccggcc aacgcgaagc tgaccggccg tgtgctcgtc
     4861 ggcgacaccg acatcaccca gctgaacttc ggcgcgctgc gtgcgctgcg ctgggcggat
     4921 gcgtcgatca tcttccaggg cgcgatgcac tcgctcaacc cggttcgcac ggtgggtcag
     4981 cagatcctcg aggctctgga actccacgtg acggactcct ggcgaaccga caaggagcgg
     5041 agggcccgcg tggccgagct gctgcgggtc gtcgacctcg cgccgcagaa gatcgacgcc
     5101 tacccgcacg agatgtcggg cggccagaag cagcgcgtga tgatcgccat ggcgctggcc
     5161 tgcgagccgg acatcatcat cgcggacgag ccgaccacgg cactcgacgt gatcgtgcag
     5221 aagcagatcc tggagatgat cagcgagctg gtcaccgatc gcggcatctc catgctgatg
     5281 atcagtcatg acctgtcggt gctcgccacc gcctgtgatc ggatcgccgt gatgcgcgac
     5341 ggcgagcttc tcgaggtcgg tgaatcgctg caggtgtgcc tcgaaccgga gcatccgtac
     5401 acccggcagc tggcagacgc cttcccgacg atcggcgacc cggcatcccg gctgaatccg
     5461 gtgacccgtc gcgaacgtcc cgcgcatgag cgcgaacagg ggatgaccga cgaggtgctg
     5521 ctcgaggcga aggggctgaa cgtcacctat cacggccgcg ggtccgctct gcgggccgtg
     5581 aagaacgtgg acctcgaggt gcatcgtggc gagatcctcg cgctggtggg tcagtccggc
     5641 tcgggaaaga gcacgctggc gcgcacgctc gtgggcctgc agcgcccgga tgacggagcg
     5701 acggtgcgct ttcgcggccg ggacctgccg aggaaggggc gcgcgctgcg tgctttccgc
     5761 agcgaggtgc agatggtgct gcaggacccc accgcagccc tgaacccgaa actgagcatc
     5821 tacgagtccg tcgccgaggg actgcgcgtg cagcactatc cgggcgacga ggcggagcgg
     5881 gtcgtgcaga gcctgatcga cgccgagctg actccgccgg agcggttcat ggctgcgatc
     5941 ccgcaggagc tgtccggcgg tcagcggcag cgggcggtga tcgcgggagc cctggcgctc
     6001 ggcccgcaga tgctggtggc cgacgagccc gtcgcctcgc tggatgcgtc ggtgcgcggc
     6061 gagatcctcg ccctgctgct ctcgctgcgc gatcgcctcg gactcagcgc cctggtgatc
     6121 acgcacgacc tggggttggc gtggaacatc gccgacaccg tcgcagtgat gctggagggc
     6181 gagatcgtgg agtacggcac gaccgagcag gtgctgctgg acccgcagca cgagtacacc
     6241 aggaagctgc tcgccgccgc tccgacgatc gagcggagcg gagcatgagc ggaaccgccg
     6301 tcgcagtgga ccggcacgcc tacctgaccg aggcgccgct gcaaccgggg gaccgggtgc
     6361 atttcatcac cccgtccgga ctcggcaccg ccgagagcct ggagcgcgca gtcggctact
     6421 accgcgactg gggcctcgag gtcaccatcg gcgagcacgt gctcgagccg catccgcggg
     6481 cgaagtatct cgccggtccg gatgccgggc gccgcggcga cctggtggat gcctggctcg
     6541 accccgacac cgccgctgtc gtatgcgtgc gcggcggcta cggggccatg cgcctgctgg
     6601 acgggatcga ctgggaactt ctgcgaggcg cggccctgcg gcgcgacgga cggccgaagc
     6661 tgctcaccgg gtcatccgac atcaccgcgc tgcacgaggc gttccgcgtg cacctcgacg
     6721 tgccgacgct gttctgcccc atgccgggca acgacgtgtt ccgggactcg gcggcgatcc
     6781 gcgacgacgt gcaccgctgg ctcttcgagc cgtggggcgg gcgtgggatc gtcgggccgg
     6841 ccgcggaggt gctcgtgccg ggtcgggcag cgggacggtt cacgggcggc aacctggcgc
     6901 tgctcgccgg tgggctcgga gcctccgagt ccgccgtggc gccggagggc atcctgttcc
     6961 tcgaggacat cgacgaggag ctctaccgcc tggacgggtt cctgctcgcc ctgctgcgcg
     7021 cggggcgcat cgccgcggca tccgggatcg tgctcggctc gtggcacaac tgcgccgtgg
     7081 cggacggggt ggcgcaactc gacggagtga aggccctgat gaccgagtac ctcgccggcc
     7141 tcggcaagcc cgtgctctgg gagcagggct tcgggcatga cccgaacgcc ctgagcgtgc
     7201 cgctgaacgt ggacggcgtg ctggaggccg gggatgccgg catccgcctg gctgtggtgg
     7261 agggagcctg atgagcgtgg agctgccgtc gctcgatgag cgcgtctcct ggtcgatccg
     7321 catcgtggat gccggcaccg gcgagctgct cgccgagcac gcgccccaca cccagtgcga
     7381 gaccgcgagc atcggcaaga tcttcctgct gatcgaggtg gctcgccgcc tcgcggaggg
     7441 atcgctgtcg gcggatcaga tcgtcccgat ccccgcggaa cacgtggtcg aggactccgg
     7501 actcctgtac cggatgctga agcagtccct gtgcgtgcat gacgcggccc tgctggtcgg
     7561 agcattcagc gacaacctgg cgaccaacgc cctgatcgcg ctgtgcgggc tggaggaggt
     7621 gcgcgcagtc gcgccatcac tcggctatgc ggacacgtcg ctgcacgact acatccgttc
     7681 ggagcgcacg cccgacctcc cgtggacacc gtcgtacggc acgggcgccg agctcgccga
     7741 cgtgatgcgc aggctgggag ccggcgatgt gatctcgccg accgtcagcg ggcaggtgat
     7801 gacctggctc gcggccaacg cagacacgtc gatggtggcc gactcactgc tgctcgatcc
     7861 gctggcgcat gtcgacgcgg agtaccagga catggtgctg cgccacaaga ccgggagcac
     7921 ctccttcgcg cgtgtcgatg tcgggcacct gaccggcccc gcgggagccc tcgcctatgc
     7981 ggtggctgcg aactggaagg ggtccgcgga ggacctgcgc gcgccggtga ccgacgcgat
     8041 gcgcgcgatc ggtgagcagc tgcgccggca cgtcacgggt cgggaccggg cagacgcgac
     8101 gatggattga ccatgggggc gccggtgcgg cgctcctcga tagcatcgac ggatgactcg
     8161 aaacggacgg ctcggcatcg gcatcatcgg cgccggcaag gtcggcccgg tggtcggggc
     8221 ggccctcgcc ggggctgggc acgcgatcac cggcatcacc tccggctcgg acgacgaccg
     8281 cgcggcggcc gtgctgccgg gagtccccgt actggacgct ctcgaggtgg tgcgtcgcag
     8341 tgagctggtc gtgatcgcgg tgccgcgcga tgagctcccc ggtctggtcg ccggcatcgc
     8401 cgagctcggc ggatggcaga tggggcagct cgtgctgcac acggatccgt cgtacggcat
     8461 cgaggtgctg cgccccgccg ccgagcgcgg cgccatcccc cttgcggtgc acccggcgat
     8521 cgcgttcaca ggcacgacga tggacctgcg gcagctgcag gcggcgttcg ccgccgtgac
     8581 cgcgcccgcc ccggtgctgc cgatcgcgca ggcactcgcc gtcgagatgg gctgcgagcc
     8641 ggtcgtcatc gccgagcagg atcgcgccgc ctacggggag gccatcgcca cggcatccga
     8701 gttctcccgc tccatcgtcg ggcaggctac ctcgctgctg cgcggagtcg gcgtggagaa
     8761 cccgggcggc tacctgtcgg cgctggtgtc gtccacggtc gagcaggcgc tgcgcgaggc
     8821 ttccggcccg ctggaaccgc cgctgccctg acctcggcgc tgacctcagc gtccgcccga
     8881 gaagcctccg ccccctccgc cgccggagaa gcccccgccg gtcgatccgc cggacgagct
     8941 cgacgcggtg taggtcgcgg cggagttcat cgacgtcgag tagttcgcca ggtgcgagtg
     9001 cagcgagacg cccgcggcca cgtccaccca gccggggccg ctccctgatg cggagtagcc
     9061 ggtctccagc accttcgtcc agctcttctc ctcgccgagc agcatggcgt aggggagcag
     9121 cttctcgtac aggtgcacca cgtccaccgt gccgtcggac cggcgttcgg cgccctgata
     9181 ggactgcagc atccggagcc ggtcggcctc ggcgacgcgg atgaactcct tcacgcccat
     9241 cagatgctcg tggcgcagcg cgccctcgta ggtcatcacc gtgtgcttgc tgaaggcgac
     9301 gatggaactg atcgccacga cgacgcccgc gccgatgagc gcgacgatgg acaggccgag
     9361 cagctcacgg tccttcatcg ccgaccaggt caggaacacg gcggtcgctg ccagcagggc
     9421 gatggatgcc cagccgaacg ccatcgcggc gcggctgcgc tcctcggtga gccagccgtt
     9481 ctgcttcgcc gcagcacggc ccttctgcac gagcttctcc atgcgcttcg cgaacttcgt
     9541 gctggacttc gggatcctcc gcacggtctc ctcgcccagg aacagggagt cgaccgccgc
     9601 ccggtcgagc ggctgcgaca cgcgtgaacg atcgatcagt ctcagcgacg gacgtcgctt
     9661 ggcatcctgt tcctcctcca ggcgcagcac cccctgcacg gcgagatgca ccatctgcgc
     9721 cgggatcggg ttcggcgccc cggggatcag cggtgcggcg gccagcgggg gcagctccgc
     9781 gggcacatcg aactgcgcga cgatgatgcc tgtcgcggtc tggcgcctcc gcttcagcgc
     9841 agcgaacgag aaccacgccc cgctcgccgt cgccatcgca ccgagcccga cgacggcggg
     9901 cccgaagtcc gcgaccggat cgggcatccg cgcctcgggc tgggtgaccg tgcccccatc
     9961 gaagccgatc gcgatcgtca tcccgtcgcc ggcggcgcgc tcgccggatt ccaccgagaa
    10021 gctcgcgccc gcggcggacg atgcgggccc gtcgagtgtg caggccccct tctggcctgt
    10081 cgtgccgctg tagcaggcgg tgtcgccggt gagcgcgtcg gccagcggcg gtgcgaaggt
    10141 gacggtggcc cggaaccggc cgatggcctg agtgctgttc agaggcagca ggttccagta
    10201 gaactcgtcg ttgcgggact tcgagccgtg gatcatgaag tcgcgcatcg tcgattcgat
    10261 gacgtacgtg ttcgagccgt gcacgtagtc gtccttccca gtgagcacga ggagcatgcc
    10321 atcctcgctc tcggtctcga acggcacatc ccgtccctcg gcgtccttga ccgacaggat
    10381 gcgcaggctg agcccggcac cctcgtaccg ctccggatag ccgcggatga tgcccttgtt
    10441 ctgatccgtc tgggggaact cggccaccac ggtctcggtc acgtgtgcca ccgcatgacc
    10501 gtcggcgtcg agggacaccc ggtagtcgga gacccaggac gagtaggaga agtcgtcgac
    10561 gtcggccgag gcggcggttg ccgggagcag tgcggcggcg atggtgccga cggccagggc
    10621 gagcaccacg aggatgcgcc ggggagcgtg cggccgtgga cggagcaggt ggggtcgcat
    10681 gctcacgagg ctatcccggc ccccgccgga gtctcaggga gggcatcggc tcgccggtag
    10741 acttgggggg actttctgca aggagccccc acccatgact gacgcgtctg cgccgatccc
    10801 ctcgcccgag ttcgaagagg acgtcttcga gcagaaggcc gtgcgtctcg ccaagcgcga
    10861 caagctgatc gcgaaccggg cggatgccgg tggcggagcg ttcccggtcg gggtgccggt
    10921 cacgcacggc atccccgcac tgcgggcgca gtacggcgac ctcgagcccg gcgccgagac
    10981 cggcgagatc gtcggcgttg cgggccgcgt ggtgttcagc cgcaacaccg gcaagctctg
    11041 cttcgccact ctgcaggccg gtgacggctc acgcatccag gcgatggtgt ccctcgcgaa
    11101 cgtcggcgag gagtcgctgg cggggtggaa ggaactcgtc gacctgggcg accacgtgtt
    11161 cgtgcacggc gaggtgatct ccagccgccg cggtgagctg tcgatcatgg ccgacgactg
    11221 ggcgatcgcg tcgaaggcca tcctgccgct gccgaacgtg tacggggagc tgaacgagga
    11281 gacgcgcgtg cgcagtcgct tcctcgacct gatcgtgcgc gaccaggccc gcaccaccgt
    11341 gcgcgcccgc gccgcggtga acgccagcct gcgcgcgacg ttcaccagcc acgactacct
    11401 cgaggtggag acgcccatgc tgcaggtgca gcacggtggg gcatccgctc gtcccttcat
    11461 cacccactcc aacgcgttcg acaccgagct gtacctgcgc atcgcgccgg agctgtacct
    11521 caagcgcgcg gtcgtcggcg gcatcgagcg ggtctacgag atcaaccgca acttccgcaa
    11581 cgagggcgcc gactcgacgc actctcccga gttcgcgatg ctcgaggcct accaggcgta
    11641 cggcgactac aaccagatgg cagagctcac gcaggagctc gtgcagaacg cggccatcgc
    11701 ggtcgccgga tccaccacgg tcacctgggc agacggcacc gagtacgacc tgggtggcga
    11761 gtgggatcgc atctccatgt acgactcgct gtccgaggtg tccggtcgca cggtcactcc
    11821 gcaggactcc gtggaggacc tcatcgcctt cgcgaacgag aacggcgtgg agatcccgcc
    11881 gcacgccact cacggcaagc tcgtcgagga gctgtgggag cacttcgtga agggtgacct
    11941 ggtccgcccc accttcgtga tggacttccc cgtcgacacc tcgccgctcg tgcgcgagca
    12001 ccgttcgatc gcgggcgtcg tggagaagtg ggacctgtac gtgcgcggct tcgagctggc
    12061 cacggggtac tccgagctga tcgacccggt gatccagcgc gagcggttcg tcgagcaggc
    12121 gaagctggcg gccggcggcg acctcgaggc catgcgcgtg gacgaggagt tcctgcgcgc
    12181 gctcgagcac ggcatgccgc cgtccggtgg catgggcatg ggcatcgacc gcctgctgat
    12241 ggccgtcacg ggtctgggca tccgcgagac catcctcttc ccgctggtca agtaggagcc
    12301 tgcggccgtg atgttcggga tcgtcgatct tgccatcgcg gtgctgctgc tcgccgccgt
    12361 ggtggcgggg ttcggtgccg gactgttctc gatactcggg gcgctgtgcg gactggtcgc
    12421 gggcgcgctc gccgtcccct tcgtgctgcc gctgatcgcg cgagcactgc cggagagcga
    12481 gtggcagggg ctgatcatcg tcgccgcggc cgtggtgctg ctggctgtcg ggatgggcat
    12541 cggttcggcg atcggggcgc tgctgcgccg cggggcggac aagctgcggc tgaagttcct
    12601 cgagcggatg ctgggcggcg tcgccgctct ggtcgccggg gcgctcgcga tcacgatggc
    12661 gggttcggcg atcgcctcgg ccggtatccc cgggctgt
//
LOCUS       DATMSX010000487         7039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_35916,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000487 DATMSX010000000
VERSION     DATMSX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35916"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="alr"
                     /locus_tag="VN041_15480"
     CDS             <1..928
                     /gene="alr"
                     /locus_tag="VN041_15480"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWU30483.1"
                     /translation="GTATLEESFALRRAGITAPLIAWLHEPGRRFDDAVEAEIEIGVS
                     SFAQLNAASETGAQASVHLKVDTGLSRNGIPAEQLEQVLAEAARLERIGRIRIVGVFS
                     HLSGASPDDDRAQLARYQEILAQAESFGIRPEIRHLAATAGAIALPEARLDAVRVGLG
                     IYGLSPFADRSSADLGLRPAMKLSAAVANVRRVTAGTGVSYDYAYHCDRDTTLALVPL
                     GYADGVPRQASGVGPVLINGRPHTVAGRIAMDQFVVDVGDERVEVGDEVVLFGDPALG
                     HPSATDWADAAGTINYEIVTRIGDRVPRRTVS"
     gene            925..1407
                     /gene="tsaE"
                     /locus_tag="VN041_15485"
     CDS             925..1407
                     /gene="tsaE"
                     /locus_tag="VN041_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWU30484.1"
                     /translation="MSIDPAFLGRHEIATSDAMERLGLHIGEQLKAGDLLVLTGPLGA
                     GKTTFTRGLAEGLGVRGPVQSPTFVIARTHPSLVGGAPLVHVDAYRLGSAAELDDLDI
                     DFGRSVVVIEWGRPMASSVADAWWDIELERPVGGVADLADEDLDADAPRFVTIERVEG
                     "
     gene            1551..2138
                     /gene="tsaB"
                     /locus_tag="VN041_15490"
     CDS             1551..2138
                     /gene="tsaB"
                     /locus_tag="VN041_15490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWU30485.1"
                     /translation="MILAVDTSLGTAVAVIDADGRTVAEASTPDPLGHAEVIGDLLAE
                     VARPGITHVIAGMGPGPFTGLRIGIAAARTFALGRGIPVIPIPSHFAIAQTADIQGRF
                     AVVTDARRREIAVSVFDGADADGIPNLVLPTRLEKRGAELAEPMHEVAELSASALAVV
                     GARAIASGRTLADAEPLYLRSPDVVQPGAPKRVSA"
     gene            2135..2623
                     /gene="rimI"
                     /locus_tag="VN041_15495"
     CDS             2135..2623
                     /gene="rimI"
                     /locus_tag="VN041_15495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWU30486.1"
                     /translation="MTLRLATGDDLAAIMDLERRSFPTDAWSEDTMRAEVASAHNHYL
                     VDEEDGRVVGYGGLRALRGGSDADIQTIALDEAVRGQGRGRALLRALIAEAVGRGARE
                     LFLEVRADNPVAEGLYRSEGFAELGRRPRYYQPDDVDAVVMRLDLRAWTVGAASEQEA
                     SA"
     gene            2620..3684
                     /gene="tsaD"
                     /locus_tag="VN041_15500"
     CDS             2620..3684
                     /gene="tsaD"
                     /locus_tag="VN041_15500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWU30487.1"
                     /translation="MTTTEPLVLGIETSCDETGIGIVRGRTLLSNTIASSMDEHARYG
                     GVVPEVAARAHLEALQPSIERALAEAQVTLDDLDAIAVTSGPGLAGALMVGVGAAKGL
                     AVSLGKPLYAVNHLVGHIAADIVAGDAEPIEYPTIALLVSGGHTSLLHVRDLTTDVEL
                     LGETVDDAAGEAFDKVARLLGLPYPGGPQIDKAAVGGDPKAIRFPRGLSKASDLEKHS
                     YDFSFSGLKTAVARWIERAEANGEEVPIADVAASFREAVVDVLVTKALAACQDRGVPR
                     LLLGGGVIANRRLREVTLERAAAAGVTVRIPPLSLCTDNGAMIAGLAAELISSGRGAS
                     TLAFGADSTLPVTEIQVSPA"
     gene            3681..4127
                     /locus_tag="VN041_15505"
     CDS             3681..4127
                     /locus_tag="VN041_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30488.1"
                     /translation="MTDPRIEGPTPEVERPEGSAALPGASKGYGKLPTAPIPVERTNH
                     EPDAEEFATRSGWEPHAESDPVPVDDARLAPWALFAAIVALATSMFVGWGIPVAIVAV
                     ISAIMSLRRPLESRAIAMWALVLGLCATLFSAGWLIWATMQFERLG"
     gene            4479..5231
                     /locus_tag="VN041_15510"
     CDS             4479..5231
                     /locus_tag="VN041_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30489.1"
                     /translation="MVAVIAIVVGLLAGRAMFGDHGPVMTAEQQDTWVRLQSMTQYDA
                     GSVELVGSKDGVDVWKATQNEGASECLLLTRDPGSPGLPGGTGVNCLNPKTALGETLG
                     ASLTYVEKGTEYSVWASSAEDINGNPFIAVQRQSMSEEWDWHVQYDKAELKTISVIEA
                     TGMDGQSLSILGYDGDTPVWLGFESQPCIVIVQPQDVVAKKCGNLALGSPPLELTVGD
                     STYSVLAGDMNSPGSGNVLTIYRAGGAAGGQG"
     gene            5228..6286
                     /locus_tag="VN041_15515"
     CDS             5228..6286
                     /locus_tag="VN041_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30490.1"
                     /translation="MSAADATRRRELQRRLYAPGGALTQEESVELQMLSAPAAVELAS
                     PQAAGGGEPGREDPSTASSALTSASAADAPELRTSEASGGSERQQDGKAHASTIDPTP
                     TRWGLIAIVAVAALALGLGIGWLTVPRSERISVPEMTSAQQKSQQAIAKTGKVDPGSL
                     EFAGTKHGAAVWTAAHGKEACLVLVLDGQRSIGCADPAKGMWGAGIIAVGAETTLDDG
                     IRRSVSATMMKDLRGERVIGIDYWDTDPDDTSWEQQYPPEDRRLIGALKEAHLAGPNL
                     MLVGRDDGVAVWAYYLDDKCVAVVDSDTLQVSKSCEPGDTSQAVQVRYGDATYSVKWT
                     ERGGATLTVFRDVAEKRI"
     gene            complement(6338..>7039)
                     /locus_tag="VN041_15520"
     CDS             complement(6338..>7039)
                     /locus_tag="VN041_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWU30491.1"
                     /translation="GAVWLDPARRTAGHSETRRLSASDWSPSLDWCFEVARSHPTGIK
                     LGPGLDRDLIPDDVEAQWVSADGSVVELVLWSGALARDGIRRSALIVAGGQTHELTAE
                     ADAEDAPVRELGAFVHEPDGAVIRARLIGDAARMLDAGMLDEQIAYLTGDAAVTSPFV
                     QSFRVRETMPAHPKTINAALRANDIGRLEIKKRGMDIDPAAFRKKLTLKGSQQATLIL
                     TRTPAGRVAILADRV"
BASE COUNT         1036 a         2434 c         2513 g         1056 t
ORIGIN      
        1 cggcacggcg actctggagg agtccttcgc cctgcgccgc gcgggcatca ccgcgccgct
       61 gatcgcctgg ctgcacgaac ccggccggcg gttcgacgac gcggtcgagg ccgagatcga
      121 gatcggcgtc tcctcattcg cacagctcaa cgcggcatcc gagaccggag cccaggcatc
      181 cgtccacctc aaggtggaca ccgggctctc ccgcaacggc atccccgccg agcagctcga
      241 gcaggtgctc gccgaggccg cccggctgga gcggatcgga cgcatccgca tcgtcggcgt
      301 cttcagccat ctctccggcg cgagccccga cgacgaccgg gcgcagctgg cccgctacca
      361 ggagatcctc gcgcaggccg agtccttcgg catccgtccg gagatccgcc acctcgccgc
      421 caccgcaggc gcgatcgcgc tgcccgaggc gcggctcgac gcggtgcgcg tgggcctcgg
      481 catctacggg ctgtcgccct tcgccgatcg gtcctcggcc gatctgggac tgcgcccggc
      541 gatgaagctc agcgcggctg tcgccaacgt ccgccgggtc accgccggca ccggcgtctc
      601 gtacgactac gcgtaccact gcgaccgcga caccacgctc gcgctcgtgc cgctcggcta
      661 tgccgacggc gtgccgcgtc aggcatccgg cgtcggcccg gtgctgatca acggacgtcc
      721 gcacaccgtc gcgggccgca tcgccatgga ccagttcgtc gtcgacgtcg gcgacgagcg
      781 cgtcgaggtg ggcgacgagg tcgtgctgtt cggcgacccg gcactcggtc atccgtcggc
      841 gaccgactgg gcggatgccg cgggcaccat caactacgag atcgtcacgc gcatcggcga
      901 ccgcgtgccc cggcggaccg tgtcatgagc atcgatcccg cgttcctggg gaggcacgag
      961 atcgccacct cggatgccat ggagcggctc ggcctgcaca tcggcgagca gctgaaggcc
     1021 ggcgacctgc tcgtgctgac ggggccgctc ggtgccggca agacgacctt cacacgcggc
     1081 ctcgccgagg ggctcggcgt gcgcggaccc gtgcagagcc cgacgttcgt gatcgcccgc
     1141 acgcatccct cgctggtcgg gggagcgccg ctcgtgcacg tggacgccta ccgtctgggc
     1201 tcggccgccg agctcgacga cctggacatc gacttcggcc gctcggtggt cgtgatcgag
     1261 tgggggcggc cgatggcctc gtcggtggcg gatgcctggt gggacatcga gctggagcgt
     1321 ccggtcggag gcgtcgccga cctggcggat gaggatctgg atgccgatgc gccgaggttc
     1381 gtgacgatcg agcgcgtcga gggctgaggc gcttgcatgt gttcgcatgg cgcggatgct
     1441 cgcttcgagc gtggcgacgc ggcgagttgg gccacgtgaa gccccaggac ccgctggtgg
     1501 agcgtgtggc tcccggccga ggcccagcgg ccgccgacta atctggaggg gtgatcctcg
     1561 ccgtcgacac ctccctgggc accgccgtcg ccgtcatcga cgccgacggg cgcaccgtcg
     1621 ccgaggcctc cacacccgac ccgctcggac acgccgaggt gatcggcgat ctgctcgccg
     1681 aggtcgcgcg ccccggcatc acgcacgtga tcgcgggcat gggtccagga cctttcaccg
     1741 gcctgcgcat cggcatcgcg gcggcgcgca cgttcgcgct cgggcgcggg atcccggtca
     1801 tcccgatccc cagccacttc gcgatcgcgc agacggcgga catccagggc cgattcgcgg
     1861 tcgtgaccga cgcccgccgt cgtgagatcg cggtgagcgt cttcgacgga gcggatgccg
     1921 acggcatccc gaacctggtg ctccccactc gcctggagaa gcgcggtgcc gaactcgccg
     1981 agccgatgca cgaggtcgcc gaactctcgg cctccgccct cgccgtcgtc ggcgcccgcg
     2041 cgatcgcctc cggccggacc ctcgccgatg ccgagcctct ctacctgcgc tcgcccgatg
     2101 tcgtgcagcc cggcgctccg aagcgggtga gcgcatgact ctccgcctcg cgaccggcga
     2161 cgacctcgcc gcgatcatgg acctcgagcg ccgcagcttc cccacggacg cgtggagcga
     2221 ggacaccatg cgtgccgagg tcgccagcgc tcacaaccac tacctcgtcg acgaggagga
     2281 cggccgcgtg gtcggctacg gcgggctgcg cgcactgcgc ggcggatcgg atgccgacat
     2341 ccagaccatc gcgctcgatg aagctgtccg cgggcagggg aggggtcgtg ccctgctgcg
     2401 cgcgctgatc gccgaggcgg tcggccgcgg tgcccgcgag ctgttcctcg aagtgcgcgc
     2461 cgacaatccc gtggccgagg gcctgtaccg gtcggagggc ttcgcggagc tggggcgccg
     2521 cccgcgctac taccagcccg acgatgtgga cgccgtggtg atgcggctcg acctgcgcgc
     2581 ctggaccgtc ggcgccgcat ccgagcagga ggcctccgca tgaccaccac cgaacccctg
     2641 gtgctcggca tcgagaccag ctgcgacgag accggcatcg gcatcgtgcg cggccgcacc
     2701 ctgctctcga acacgatcgc gtcgagcatg gacgagcacg cccggtacgg cggcgtcgtg
     2761 cccgaggtcg ccgcgcgtgc gcatctggag gccctgcagc cgtcgatcga gcgcgctctc
     2821 gccgaggcgc aggtgaccct cgacgacctg gacgccatcg ccgtgaccag cggtcctggc
     2881 ctggccggag ccctcatggt cggcgtgggc gctgccaagg gcctcgcggt gtctctgggg
     2941 aagccgctgt acgcggtgaa ccatctggtc gggcacatcg ccgctgacat cgtcgccggg
     3001 gatgcggagc ccatcgagta cccgacgatc gccctgctcg tcagcggagg ccacacctcg
     3061 cttctgcacg tgcgcgatct cacgaccgat gtcgagctgc tcggcgagac cgtcgacgac
     3121 gccgccggcg aggccttcga caaggtcgcg cgtctgctgg ggctcccgta ccccgggggg
     3181 ccgcagatcg acaaggccgc ggtcggcggc gacccgaagg cgatccgctt cccacgaggg
     3241 ctgtccaagg catccgatct ggagaagcac agctacgact tctcgttctc ggggctgaag
     3301 accgcggtcg cccgctggat cgagcgcgcc gaggccaacg gcgaggaggt ccccatcgcc
     3361 gatgtcgccg ccagcttccg cgaagcggtc gtcgacgtgc tggtgaccaa ggcgctcgcc
     3421 gcctgccagg accgaggcgt ccctcgcctg ctgctgggcg gtggcgtgat cgccaatcgg
     3481 cgtctgcgcg aggtgacgct ggagcgtgcc gccgccgcgg gcgtcacggt gcgcatccct
     3541 ccgctgagcc tgtgcacgga caacggcgcg atgatcgccg gcctcgcggc cgagctgatc
     3601 tcgagcggcc gtggcgcgtc gacactggcg ttcggggcgg actcgacgct gcccgtgacc
     3661 gagatccagg tgagcccggc atgaccgatc cgcgcatcga gggacccact cccgaggtcg
     3721 agcgacctga ggggagtgcg gcgctgcccg gtgcgtcgaa gggctacggc aagctgccga
     3781 ccgcgccgat cccggtcgag cggaccaacc acgagccgga tgccgaggag ttcgcgacgc
     3841 ggtcgggctg ggagccgcac gcggagtcgg acccggtgcc ggtggatgac gcccggctcg
     3901 cgccgtgggc cctgttcgcc gcgatcgtcg cgctggcgac gtcgatgttc gtggggtggg
     3961 ggataccggt ggcgatcgtc gccgtgatct cggcgatcat gtcgctgcgg cgcccgctgg
     4021 agagccgcgc gatcgcgatg tgggctctgg ttctcggact gtgtgccacg ctgttcagtg
     4081 cgggctggct gatctgggcg accatgcagt tcgagagact ggggtgacgg atgctggagg
     4141 gggcggaagc tgcacgacga cgtgagcttc aggtgcgggc ctacgcaccc gggggctcgc
     4201 tgacggatgc cgaggccgct gagctgcggg atctggacgc gcgcaatctg acggctcccg
     4261 tgatcgcgcc cccggtcccc gagccgacgc cgactcgcga tgacgggttc cgagcctccg
     4321 cgctccggac ggatccttcc actctgaagg ggtcatctgt tgagcacgcg gagatgcctc
     4381 ctgcttcgga gactccgaac cttccgccgg acgaagttcc cgccgctggc cgaacgacga
     4441 tgagcctccg cagcatcccc cgccgcgtaa tcgtgccggt ggtcgccgtg atcgcaatcg
     4501 tggtgggatt gctcgcgggt cgggccatgt tcggcgacca cgggcccgtg atgacggctg
     4561 aacagcagga cacctgggtg aggctccagt cgatgactca atatgacgcc ggttctgtcg
     4621 agctcgtcgg cagcaaagac ggcgtggatg tctggaaagc gacgcagaac gagggtgcat
     4681 cggagtgcct gcttctgacg cgcgacccgg gctctccggg tctgccaggt ggcacgggcg
     4741 tcaattgcct caacccgaag accgctttgg gggagaccct gggtgcaagc ctgacttacg
     4801 tcgagaaggg caccgagtat tcagtatggg catcctcggc cgaggacatc aacggcaacc
     4861 cgttcatcgc ggtgcagcgc cagagcatgt ctgaggagtg ggactggcac gtgcaatacg
     4921 acaaggcgga gctgaagacg atcagtgtga tcgaggccac cgggatggac ggacagagcc
     4981 tcagcatcct cggctacgac ggcgacactc ccgtgtggct cggcttcgag agccagccgt
     5041 gcatcgtgat cgtgcagccg caggacgtgg tcgccaagaa gtgtggaaat ctcgcgctcg
     5101 gctcgccgcc gctggaactg accgtggggg acagcaccta ctcggtgctc gccggcgaca
     5161 tgaacagtcc cgggagcggc aacgtcctga cgatctatcg cgccgggggc gcggcggggg
     5221 gacagggatg agtgcagcgg atgccacgcg ccggcgcgag ctgcagcgcc gtctgtacgc
     5281 ccctggcggt gctctgacgc aggaggagag cgtggagttg cagatgctct cggctcccgc
     5341 ggccgtcgag ctcgcgagcc cgcaggcagc gggcggcggg gagccggggc gggaggatcc
     5401 gtccacagcc tcgtcagccc tgacctccgc ctccgcggcc gacgcgcctg agcttcgcac
     5461 atcggaggcg tcaggcggct cggagcggca gcaggacggg aaggctcacg catccacgat
     5521 cgaccccacc cccacccgct gggggctgat cgcgatcgtg gccgtcgctg cactcgcgct
     5581 cggtctcggc atcggttggc tgacggtgcc acggtctgag cggatatccg tccccgagat
     5641 gacctcggcg cagcagaagt cccagcaggc gatcgcgaag acgggaaagg tcgatcccgg
     5701 ctctctcgag ttcgccggca cgaaacatgg ggcggctgtc tggacggccg ctcatggcaa
     5761 ggaagcatgt ctcgttctcg tgctcgacgg ccagcggagt atcggctgcg ccgaccccgc
     5821 caaggggatg tggggtgcgg gcatcatcgc ggtcggtgcc gagaccactc tggacgacgg
     5881 catcaggcga tctgtgtctg cgacgatgat gaaggatctg cgcggcgagc gggtgatcgg
     5941 aatcgactac tgggacaccg acccggacga cacctcctgg gagcagcagt atccgccgga
     6001 ggaccggcgg ctcattggcg cgctgaaaga ggcccatctc gccggcccga atctgatgct
     6061 cgtcggccga gacgacggcg tcgccgtgtg ggcgtactac ctcgacgaca agtgcgtcgc
     6121 ggttgtcgat tctgacactc tccaggtgtc gaagagctgc gagcccggcg atacatccca
     6181 agctgtgcag gtgcggtacg gcgatgcgac ctacagcgtg aagtggaccg aacgaggcgg
     6241 agcaaccttg acggtcttcc gcgacgtggc cgagaagagg atctgacccg agctcggctg
     6301 cagggccgac ctggtggctg acgggagaga ccgctgatca gacccggtct gcgaggatcg
     6361 ccacgcgtcc ggccggtgtc cgggtgagga tcagcgtcgc ctgctgtgag cccttcagcg
     6421 tcagcttctt ccggaacgcc gccgggtcga tgtccatgcc gcgcttcttg atctcgagcc
     6481 ggccgatgtc gttcgccctg agcgccgcgt tgatggtctt gggatgcgca ggcatggtct
     6541 cccgcacccg gaacgactgc acgaacgggc tcgtcacggc ggcgtcgccg gtgagatagg
     6601 cgatctgctc gtcgagcatc ccggcatcca gcatccgtgc cgcgtcgccg atcagccgcg
     6661 cacggatcac ggccccgtcc ggttcgtgca cgaatgcgcc gagctcgcgg accggtgcgt
     6721 cctcggcatc cgcttccgcg gtcagctcgt gggtctggcc gccggccacg atcagcgccg
     6781 accggcggat gccgtcgcgg gcgagcgcgc cgctccacag cacgagttcg accacgctgc
     6841 cgtcggcgct gacccactgc gcctcgacgt cgtcggggat gagatcgcgg tccagccccg
     6901 gtccgagctt gatccccgtc gggtggctgc gtgcgacctc gaagcaccag tccagggacg
     6961 gcgaccagtc ggatgcggac agccggcgcg tctcgctgtg cccggcggtg cgccgggcgg
     7021 ggtccagcca caccgcgcc
//
LOCUS       DATMSX010000488         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_130925,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000488 DATMSX010000000
VERSION     DATMSX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130925"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VN041_15525"
     CDS             complement(<1..560)
                     /locus_tag="VN041_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30492.1"
                     /translation="MTTMQDVARHAGVSVMTVSNVINEHPHVRDSTREKVIASIAELG
                     YHVNTSARSLRQGRTGVIALAVPEIDRPYFGLLASLLIERAAARGFELVVEQTAARRD
                     RELDAISRSRLRNYDGLILSAVELHDDDATLLRGDFPTVILGERGFTGPLDHVTMANE
                     EGAALAAAHLVERGCRTVAMLGGYHSP"
     gene            complement(597..2042)
                     /locus_tag="VN041_15530"
     CDS             complement(597..2042)
                     /locus_tag="VN041_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWU30493.1"
                     /translation="MIALSRAAGAPVIDRRIYGQFAEHLGRCIYEGVWVGQDSAIPHR
                     NGLRIDVIDALRHIKVPLVRWPGGCFADEYHWLKGVGPERQPMVNTHWGGVVESNEFG
                     THEYFELLDQLDAEAYINGNVGSGTVAEMQDWIEYMTLDSTAPMAELRKRHGREQPWK
                     LEFFGVGNESWGCGGNMLPLHYANQYRQFQTYVRKYGEHPISKIACGANSDDYEWTEV
                     LMARAAEHMNGLSLHYYTLPTSDWERKGAALDFTDFEWHETLRQARRMDELITRHSRI
                     MDRYDPAKRVALIVDEWGTWYDVEEGTNPGFLYQQSTMRDAAVAAITLNIFHRHADRV
                     RMTNIAQMVNVLQAMILTDGARMLTTPTYHVFDLYTDHQDAEYVDVPVGAFGADVDAT
                     ASFKDGAYTVSITSTALDREIDVEFELPSTPIEVLRAQVLGGPSATAHNTFDAPSAVA
                     PVELAVAVQGRTVKTTLPPHAVATVRLAVAD"
     gene            2098..>3465
                     /locus_tag="VN041_15535"
     CDS             2098..>3465
                     /locus_tag="VN041_15535"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWU30494.1"
                     /translation="MKFLPLDRVRLLEGPFARALETDLGYALALDPDRLLAPFRREAG
                     LPAKAPGYGNWESSGLDGHIAGHLLSALAFLWAGTGDAEARRRLDAMLAGLAECQASN
                     GGGYVGGIPGGQALFEALATGGTAVAELGSSEHWVPWYNLHKTFQGLIDAHIVTGDEQ
                     PLRVVTALADWWLPIADRIDDAAFERMLDTEFGGMNDAFARLAQLTGRAEYAAQAERF
                     SHRAILDPLLRGEDALTGLHANTQIPKAVGYASTAVATGEDSYLRAAEAFWRTVVEHR
                     TVAIGGNSVREHFHDAHDFTPMIDDREGPEFCNTYNMLGLTRALAERELRPELLDFAE
                     RAIFNHVLASQHPERGGFVYFTPLRPRHYRVYSQAKLGFWCCVGTGLEAQARSAAEWV
                     FGVEDGSPAVNLYLPAEASIDLPDAALPGVRIRVDADLPHSGEVGISVDPDGSRAFAL
                     RLRVPA"
BASE COUNT          497 a         1190 c         1206 g          572 t
ORIGIN      
        1 gggctgtgat agccgccgag catcgcgacg gtgcggcagc cgcgctcgac gaggtgcgcc
       61 gccgcgagcg cggcaccctc ctcgttggcc atggtgacgt gatcgagcgg gcccgtgaac
      121 ccgcgctcgc ccaggatgac cgtggggaag tcgccccgca gcagcgtcgc gtcgtcgtcg
      181 tgcagctcga ccgcgctgag gatcaggccg tcgtagttgc gcaggcgcga ccgcgagatg
      241 gcgtccagct cccggtcgcg cctggcggcg gtctgctcca ccaccagctc gaacccgcgt
      301 gcggcggcgc gttcgatgag caggctggcc agcaggccga agtaggggcg atcgatctcg
      361 ggcacggcaa gggcgatgac gccggtgcgg ccctgccgca ggctgcgcgc ggaggtgttg
      421 acgtggtagc cgagctcggc gatcgaggcg atcaccttct cgcgggtgct gtcgcgcacg
      481 tgcggatgct cgttgatcac gttcgacacc gtcatcaccg acacccccgc gtgcctggcc
      541 acgtcctgca tcgtcgtcat gtcgtctctt cctccgggcc tctcgatccc agggaatcag
      601 tctgcgacag cgagacgcac ggtggcgacc gcgtgcggcg gcagagtcgt cttcacggtg
      661 cggccctgca cggcgacggc cagctcgacc ggagccacgg cggacggcgc gtcgaaggtg
      721 ttgtgcgccg tggcggatgg gccgccgagc acctgtgcgc gcagcacctc gatgggggtc
      781 gagggcagct cgaactccac gtcgatctcg cgatccagcg cggtgctcgt gatgctcacc
      841 gtgtacgcgc cgtccttgaa cgatgcggtc gcgtcgacgt cggcgccgaa cgcccccacg
      901 ggcacgtcga cgtactctgc atcctggtga tccgtgtaca ggtcgaacac gtggtaggtg
      961 ggtgtggtca gcatccgcgc gccgtcggtg aggatcatgg cctgcagcac gttgaccatc
     1021 tgcgcgatgt tggtcatccg cacccggtcg gcatggcggt ggaagatgtt cagcgtgatc
     1081 gccgcgaccg cggcatcccg catcgtgctc tgctggtaca ggaagcccgg gttggtgccc
     1141 tcttcgacgt cgtaccaggt gccccactcg tccacgatca gcgcgacccg cttcgccggg
     1201 tcgtaccggt ccatgatgcg actgtgccgg gtgatcagct cgtccatgcg acgcgcctga
     1261 cgcagcgtct cgtgccactc gaagtcggtg aagtcgaggg cggcgccctt cctctcccaa
     1321 tccgaggtgg gcagcgtgta gtagtgcagg ctcaggccgt tcatgtgctc ggcggcgcga
     1381 gccatgagca cctcggtcca ctcgtagtcg tcggagttcg cgccgcaggc gatcttcgag
     1441 atcggatgct cgccgtactt gcgcacgtag gtctggaact gccggtactg gttcgcgtag
     1501 tgcagcggga gcatgttgcc gccgcagccc cagctctcgt ttccgacgcc gaagaactcc
     1561 agcttccacg gctgctcacg gccgtggcgc ttgcgcagct cggccatcgg cgccgtggag
     1621 tcgagggtca tgtactcgat ccagtcctgc atctcggcca ccgtgccgct gccgacgttc
     1681 ccgttgatgt acgcctcggc atcgagctga tccagcagct cgaagtactc gtgggtgccg
     1741 aactcgttcg attcgacgac gccgccccag tgcgtgttca ccatcggctg acgctccgga
     1801 ccgacgccct tgagccagtg gtactcgtcg gcgaagcagc cgccgggcca gcgcaccagc
     1861 ggcaccttga tgtgccgcag ggcgtcgatg acgtcgatgc gcagtccgtt gcggtgcggg
     1921 atggcggagt cctgccccac ccacacgccc tcgtagatgc accggccgag atgctcggcg
     1981 aactggccgt agatgcgtcg gtcgatgacg ggtgcgcctg cggcacggga gagagcgatc
     2041 atgcgcgtcc tcggaagaag tgggtcgata cgggaattgt aacgctacag tattcgcatg
     2101 aagttcctcc ccctggatcg ggtccgcctg ctcgagggcc cctttgccag ggcgctcgag
     2161 accgacctgg gctatgccct cgctctggat cccgaccggc ttctcgctcc gttccggagg
     2221 gaggcgggcc tgcccgcgaa ggcgccgggc tacggcaact gggagagctc cgggctcgac
     2281 ggccacatcg ccgggcatct gctctcggcg ctcgcgttcc tctgggcggg caccggcgac
     2341 gccgaggccc gccgacgcct cgacgcgatg ctcgccggac tcgcggagtg ccaggcctcg
     2401 aacgggggcg gctacgtcgg cggcatcccg ggcggacagg cgctgttcga ggcgctcgcc
     2461 accggcggga ccgccgtcgc cgagctcggc tccagcgagc actgggtccc ctggtacaac
     2521 ctgcacaaga cgttccaggg cctcatcgac gcgcacatcg tgaccggtga cgagcagccg
     2581 cttcgggtcg tgacggcgct ggcggactgg tggctgccga tcgcggaccg gatcgacgac
     2641 gccgcgttcg agcggatgct ggacaccgag ttcggcggaa tgaacgacgc cttcgcccgg
     2701 ctggcgcagc tgaccggtcg cgcggagtac gccgcacagg ccgagcgctt ctcgcatcgc
     2761 gcgatcctcg atccgctgct gcgcggcgag gacgccctga ccggcttgca cgcgaacacc
     2821 cagatcccga aggcggtcgg ctacgcgagc acggcggtcg cgaccggcga agactcgtac
     2881 ctgcgagccg ccgaggcgtt ctggcgcacc gtcgtcgagc accgcacggt cgcgatcggc
     2941 ggcaacagcg tgcgcgagca cttccacgac gcgcacgact tcaccccgat gatcgacgat
     3001 cgcgagggcc ccgagttctg caacacctac aacatgctcg ggctcacgcg agccctcgcc
     3061 gagcgggagc tgcgaccgga actgctggac ttcgccgagc gcgcgatctt caatcatgtg
     3121 ctcgcctcgc agcatcccga gcgcggcggc ttcgtgtact tcaccccgct gcggccgcgg
     3181 cattaccggg tgtactcgca ggcgaagctc gggttctggt gctgcgtggg caccgggctc
     3241 gaggcgcagg cgcggtcggc cgcggaatgg gtgttcggcg tcgaggacgg ctcgcccgcg
     3301 gtcaacctgt acctgcctgc ggaggcgtcg atcgatctgc cggatgccgc tctccccggc
     3361 gtccgcatcc gggtcgacgc cgatctgccg cacagcggag aggtcggcat cagcgtcgac
     3421 ccggatggct cgcgcgcgtt cgcgctgcga ctgcgcgtgc cggca
//
LOCUS       DATMSX010000489         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_102226,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000489 DATMSX010000000
VERSION     DATMSX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102226"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VN041_15540"
     CDS             complement(<1..1098)
                     /locus_tag="VN041_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HWU30495.1"
                     /translation="MARSTTKSERASATPASRPKRQTTSRSGAKAAPAPKKYVGEADK
                     PPVGVRMWLGIAHGVGALFRAFGPESLEKDDRRDGFPFLLVLLACAGAVVEWFFIGNE
                     IAQSISAYTWGLLVGRVAFVLPVLLLFLAGWLFRHPSSVHDNGRIGIGFGFFVLAMAG
                     FCHVAAGTANSLGEIVRPQPKDGMPALSAAGGLFGWMLGEPLAHLTAVVAYIVLGILA
                     VLSILILTKTPPNRIGERLGDLYSWMFGAERPLRAAAVDEAPKDEDDSAKDELPWWRR
                     NKTGREEDPDDGVDSKAITALFDEPAGDGAYEQAVVVDPAPQDAATEVLSDISALTGL
                     LDEQNTTALLDDTGSTGELPGLDGFGTAGPGA"
     gene            complement(1158..2834)
                     /locus_tag="VN041_15545"
     CDS             complement(1158..2834)
                     /locus_tag="VN041_15545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWU30496.1"
                     /translation="MSIPIAAPEPLAQGTLRVTPLGGLGEVGRNMTTFEFGGKILIVD
                     CGVLFPEEHQPGVDLILPDFEPIKHRLDDIVGVVLTHGHEDHIGAVPYLLRLKQDIPL
                     IGSGLTLALVEAKLKEHRIKPYTLTVTEGQQESVGPFDLEFIAVNHSIPDALAVAIRT
                     PAGLALATGDFKMDQLPLDGRITDLRAFARLGEEGVDLFLVDSTNADVPGFTPTERSI
                     GPVLDQVIGKAPRRVIVASFSSHVHRVQQVIDAAHAHGRRVAFLGRSMVRNMTIAEQL
                     GYLHVPDNVLIDYKKARDLPDDRIVYMSTGSQGEPMAVLSRMANLDHAIEPGPGDTVI
                     LASSLIPGNENAVYRVIDGLTKLGANVVHKANARVHVSGHAAAGELLYCYNILKPKNV
                     LPVHGEYRHLMANARLAQDTGIAEENTIIGSNGTVVDLKDGKATVAGQHDIGFVYVDG
                     STVGEITDADLKDRRILGEEGFVSVIVVVDSATGRIISGPEIHARGVAEDDSVFDDVA
                     PKIIAALKEAAGNGVRDNHALSQIVRRTIGRWVNQKLRRRPMIVPLVIEA"
     gene            complement(2899..3876)
                     /gene="dapA"
                     /locus_tag="VN041_15550"
     CDS             complement(2899..3876)
                     /gene="dapA"
                     /locus_tag="VN041_15550"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWU30497.1"
                     /translation="MTHIDNPFGQVLVALVTPMTADGEVDWPAVEKHLDRVISDGADG
                     IVVTGTTGETSTLTDAEKIKLVEVGKDVSAGRAKIITGGGSNETAHAIELYKASEKAG
                     ADGIMIVTPYYNKPTQAGILTHFRLVADATDLPVILYDIPGRTGVPIKYETILRLAKH
                     PNILAVKDAKGDFSEVSRVLNQTDLMYFSGDDANVLPHLAIGATGMIGVTANITSAPY
                     RTIVDAVNRGDLKTATAEHKRLEPLVRAVMTHVPGTVSAKYILHGLGRIGSPRVRLPL
                     VGPEEWEAAIIEDELALVHDVPGADFSNFRPDRNAAAGGALPKVHGTTR"
     gene            complement(3968..4729)
                     /locus_tag="VN041_15555"
     CDS             complement(3968..4729)
                     /locus_tag="VN041_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU30498.1"
                     /translation="MPTALITGASAGLGAEFARQLAAKGADLVLVARGTDALNALAGE
                     LRARHGVAVEMLAADLAQESDVTRVAERIADAEHPIDLLVNNAGFGLPLQFADNDIDD
                     EVRHLRVHVEASMRLMHAGIRAMRGRGGRIINVASVAGFISRSTYSACKSWLIGFSRW
                     ANAEYSRDGVSVTAVCPGFTHTSFHARMGLSPGHEGVPGPMWLDAADVVREGLRDAAR
                     GKSVSIPSLRYKVIVALSRVLPSSLTAGVARRGRV"
     gene            complement(4729..5205)
                     /locus_tag="VN041_15560"
     CDS             complement(4729..5205)
                     /locus_tag="VN041_15560"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWU30499.1"
                     /translation="MSWAGLIWAEAEGGVIGAAGGMPWHVPEDLAHFKEITLGDPVIM
                     GRRTWESLPERFRPLPGRENIVITRQQDWAAEGVRRAADIDEAVRGLDRMWFIGGSDI
                     FRQVIGQADRLEVTELDLRVDGDAFAPSREGWRLVDEGEWETSRTGIRYRFLGYER"
     gene            complement(5202..6011)
                     /locus_tag="VN041_15565"
     CDS             complement(5202..6011)
                     /locus_tag="VN041_15565"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWU30500.1"
                     /translation="MSAAVPTPYEDLLRDVLEHGTHKDDRTGTGTTSVFGRQIRFDLS
                     QGFPLITTKRVHFKSIAYELLWFLRGESNVRWLQEHGVSIWDEWADADGDLGPVYGVQ
                     WRSWPTPDGESIDQLSQVIEQIRATPDSRRLIVSAWNPADIPDMALAPCHAMFQFYVA
                     DGKLSCQLYQRSADLFLGVPFNIASYALLTLMVAQQTGLEPGDFVWTGGDCHIYDNHV
                     DQVREQLTREPFPYPTLNITRTPESILDYRYEDFEVVGYQHHPAIRAAVAV"
     gene            6108..6533
                     /locus_tag="VN041_15570"
     CDS             6108..6533
                     /locus_tag="VN041_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWU30501.1"
                     /translation="MSPIPALLVLAALVVIAAVAGVVWRARDGRRRRGSDVVDLTDLG
                     VTPGRVTLVQFGTETCARCPQVRRMLQGIASALPTVDHVDVDLTHRADLARAHRVLGT
                     PTTFLIGSDAVLVARFNGVPRRADVEAALSELPVLQEAS"
     gene            6530..>6825
                     /locus_tag="VN041_15575"
     CDS             6530..>6825
                     /locus_tag="VN041_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HWU30502.1"
                     /translation="MTETAPATPRGIDPRAPRFAAWITSVLLLVATFLGLIGIGGSRF
                     DPAFVLTVVIALLFLWGVLSPTTSPWSVLFRRVVRPRLAPPTDLEDPRPPRFAQG"
BASE COUNT          980 a         2328 c         2407 g         1110 t
ORIGIN      
        1 cgcgccgggg ccggccgtgc cgaaaccgtc cagcccggga agctcgccgg tggagccggt
       61 gtcgtcgagc agcgccgtgg tgttctgctc gtcgagcagc ccggtcagcg cgctgatgtc
      121 actgaggacc tcggtcgcgg catcctgcgg agccggatcg accacgacgg cctgctcgta
      181 cgcgccgtcg ccggcgggtt cgtcgaagag ggcggtgatc gccttcgagt cgaccccgtc
      241 gtcgggatcc tcctcgcggc ccgtcttgtt gcgtcgccac cagggcagct cgtccttggc
      301 gctgtcgtcc tcgtccttcg gggcctcgtc gacggcggcg gcacgcagag ggcgctccgc
      361 cccgaacatc caggagtaca ggtcacccag ccgctcgccg atgcggttcg gcggggtctt
      421 ggtgaggatc aggatgctga gcaccgcgag tatgccgagc acgatgtacg ccacgacggc
      481 ggtgaggtgg gccagcggct cgcccagcat ccagccgaac agaccgccgg ctgcgctgag
      541 cgccggcatc ccgtccttcg gctgcggtcg gacgatctcc cccaggctgt tcgccgtgcc
      601 ggcagccaca tggcagaagc cggccatggc gagcacgaag aagccgaagc cgatcccgat
      661 ccggccgttg tcgtgcaccg acgacggatg acggaacagc cacccggcga ggaacagcag
      721 cagcacgggc agcacgaacg ccacccgccc gacgagcagg ccccaggtgt aggcgctgat
      781 ggactgcgcg atctcgttgc cgatgaagaa ccactcgacg accgctccgg cgcatgccag
      841 cagcaccagc aggaacggga agccgtcgcg ccggtcgtcc ttctcgaggg actccggacc
      901 gaaggcgcgg aacagggcac cgacgccgtg cgcgatgccg agccacatcc gcacgccgac
      961 gggcggcttg tcggcctcgc ccacgtactt cttcggtgcc ggcgcggcct tcgcaccgga
     1021 gcgcgaggtc gtctgccgct tcggacgcga cgcgggcgtc gccgacgcgc gctcagactt
     1081 cgtggtgctc ctggccatgc ctcaacggta cggccaggag ccgacatccg gccgtaatga
     1141 cgcgggtttc cgcgggatca ggcctcgatg acgagcggca cgatcatcgg gcgccgacgc
     1201 agcttctggt tcacccagcg gccgatcgtg cgacgcacga tctgcgagag cgcatggttg
     1261 tcgcgcacgc cgtttccggc ggcctccttg agggccgcga tgatcttcgg cgccacgtcg
     1321 tcgaagacgc tgtcgtcctc ggcgacgccc cgggcgtgga tctcgggacc ggagatgatg
     1381 cgtccggtgg cggagtccac caccacgatc accgacacga agccctcctc gccgaggatg
     1441 cggcggtcct tcagatcggc gtccgtgatc tcgccgaccg tggacccgtc gacgtacacg
     1501 aacccgatgt cgtgctgccc cgcgacggtc gccttgccgt ccttcaagtc gacgaccgtg
     1561 ccgttggagc cgatgatggt gttctcctcg gcgatgccgg tgtcctgcgc gagccgggcg
     1621 ttcgccatca gatggcggta ctcgccgtgc acgggcagca cgttcttcgg cttgaggatg
     1681 ttgtagcagt acagcagctc gccggcggcg gcatggccgg agacgtgaac cctggcattg
     1741 gccttgtgca cgacgttcgc gccgagcttg gtgagcccgt cgatcacgcg gtacacggca
     1801 ttctcgttgc cggggatcag gctggaagcg aggatgacgg tgtcgccggg ccccggctcg
     1861 atggcgtggt cgaggttggc catccggctg agcacggcca tcggctcgcc ctgcgagccg
     1921 gtcgacatgt agacgatccg gtcgtcggga aggtcgcggg ccttcttgta gtcgatgagg
     1981 acgttgtcgg ggacgtgcag gtagcccagc tgctcggcga tggtcatgtt gcgcaccatg
     2041 ctgcggccga ggaacgcgac acggcggccg tgcgcgtgcg cggcgtcgat gacctgctgc
     2101 acgcggtgca cgtggctcga gaagctcgcc acgatcacgc ggcgcggcgc cttgccgatc
     2161 acctggtcga gcaccgggcc gatcgagcgc tcggtgggcg tgaagccggg gacgtccgcg
     2221 ttcgtggagt cgaccaggaa caggtcgacg ccctcctcgc ccaggcgggc gaaggcgcgc
     2281 aggtcggtga tgcggccgtc cagcggcagc tggtccatct tgaagtcgcc ggtcgcgagc
     2341 gccagacccg cgggcgtgcg gatcgcgacg gcgagtgcat ccgggatgga gtggttcacc
     2401 gcgatgaact cgaggtcgaa aggaccgacg ctctcctgct gcccctcggt caccgtcagc
     2461 gtgtacggct tgatgcgatg ctccttgagc ttcgcctcca ccagcgcgag ggtcagcccg
     2521 gagccgatca gcgggatgtc ctgcttgagc cgcagcaggt agggcaccgc gccgatgtgg
     2581 tcctcgtggc cgtgcgtgag cacgacgccg acgatgtcgt cgaggcggtg cttgatgggc
     2641 tcgaagtcgg ggaggatcag gtcgacgccg ggctggtgct cctcggggaa cagcacaccg
     2701 cagtcgacga tgaggatctt gccgccgaat tcgaaggtgg tcatgttgcg gccgacctcg
     2761 ccgagaccgc cgaggggcgt cacgcggagc gtgccctggg cgagcggttc gggagcggcg
     2821 atggggatgg acatgggtgt ctcctcagtc ggacaccgag tgcccgttcg tggtcgggcg
     2881 ctccacgcgc ccgggatgtc agcgcgtggt gccgtgcacc ttcggcagtg cgccgccggc
     2941 ggcggcgttg cggtcgggac ggaagttgga gaagtctgcg ccgggcacgt cgtggaccag
     3001 cgccagctcg tcctcgatga tcgcggcttc ccactcctcc ggtcccacca gcggcaggcg
     3061 gactcggggg ctgccgatgc ggcccagccc gtgcaggatg tacttggccg agacggtgcc
     3121 cggcacgtgg gtcatgaccg cgcgcaccag cggctcgagg cgcttgtgct cggcggtcgc
     3181 ggtcttcaga tcgccgcggt tcacggcgtc gacgatcgtg cggtacggcg ccgacgtgat
     3241 gttcgccgtg acgccgatca tcccggtcgc tccgatcgcg aggtgcggga gcacgttcgc
     3301 gtcgtcaccg gagaagtaca tgaggtctgt ctggttgagc acgcggctga cctcggagaa
     3361 gtcgcccttg gcgtccttca cggcgaggat gttcgggtgc ttggccaggc gcaggatcgt
     3421 ctcgtacttg atcggcacac ccgtgcgtcc cgggatgtcg tacaggatga ccggcagatc
     3481 ggtcgcgtcc gcgaccagcc ggaagtgggt caggatgccg gcctgggtcg gcttgttgta
     3541 gtacggcgtg acgatcatga tgccgtcggc tccggccttc tcgctggcct tgtagagctc
     3601 gatcgcgtgc gcggtctcgt tggagccgcc cccggtgatg atcttcgcgc gaccagcgga
     3661 gacgtccttg ccgacctcga cgagcttgat cttctcggcg tccgtcagcg tggaggtctc
     3721 gccggtggtt ccggtgacga cgatgccgtc ggcgccgtcg ctgatgacgc gatccaggtg
     3781 cttctcgaca gcgggccagt ccacttcgcc gtcggcggtc atcggggtga ccagcgcgac
     3841 gaggacctgc ccgaacggat tgtcgatgtg cgtcatggat tcaggttatc gcgtcggatg
     3901 ccgtgacatg cgcccgatgc ggtgcgcatg acggcctgct cccactgccg ggaccgcgcg
     3961 gagaggctca gacgcgtccc cggcgggcga cgcctgcggt caacgagctc ggcagcacgc
     4021 gcgacagggc gacgatgacc ttgtaccgga gcgacgggat cgacacggat ttgcctcgcg
     4081 ccgcgtcccg cagtccctcg cgcacgacgt ccgcggcgtc cagccacatc ggccccggca
     4141 cgccctcgtg tcccggcgag agccccatcc gcgcgtggaa gctggtgtgc gtgaaaccgg
     4201 ggcagaccgc ggtcacgctc acgccgtccc tcgagtactc ggcattcgcc cagcggctga
     4261 acccgatgag ccacgacttg caggccgagt aggtggagcg cgagatgaac cctgccaccg
     4321 aggcgacgtt gatgatgcgt ccgccgcggc cccgcatggc gcggatcccg gcgtgcatga
     4381 gacgcatcga cgcctcgacg tgcacgcgca gatggcgcac ctcgtcatct atgtcgttgt
     4441 cggcgaactg cagcggcagc ccgaagccgg cgttgttgac gagcaggtcg atcggatgct
     4501 cggcatccgc gatccgctcg gcgacacggg tgacgtcgga ctcctgagcg agatcagccg
     4561 cgagcatctc gaccgcgacg ccgtgccgcg cgcgcagttc acctgccagg gcgttcaggg
     4621 catccgtccc gcgcgccacg agaacgagat ccgcgccctt cgccgccagc tgacgcgcga
     4681 actcggcgcc cagaccggcg ctggcgcccg tgatcagcgc ggtgggcatc agcgctcgta
     4741 tcccaggaag cggtagcgga tgcccgtgcg ggaggtctcc cactccccct cgtcgaccag
     4801 tcgccagccc tcccgcgagg gggcgaatgc gtcgccgtcg acccgcaggt ccagctcggt
     4861 gacctccagc cgatcggcct gaccgatcac ctggcggaag atgtcggaac cgccgatgaa
     4921 ccacatgcga tcgagcccgc ggaccgcctc gtcgatgtcg gccgcgcgcc gcacgccttc
     4981 cgccgcccag tcctgctgcc gggtgatgac gatgttctcc cggcccggga ggggacggaa
     5041 ccgctccggg agcgactccc aggtgcgccg gcccatgatc accgggtcgc cgagagtgat
     5101 ctccttgaag tgcgcgaggt cctcgggcac atgccagggc atcccgcctg cagcgccgat
     5161 gacgccgcct tcagcctcgg cccagatcag cccggcccag ctcatacggc caccgccgcg
     5221 cggatcgcgg ggtggtgctg gtagccgacc acctcgaagt cctcgtagcg gtagtcgagg
     5281 atcgactccg gggtgcgcgt gatgttcagc gtcggatacg ggaacggctc gcgcgtgagc
     5341 tgctcgcgca cctggtcgac gtggttgtcg tagatgtggc agtcaccgcc ggtccacacg
     5401 aagtcaccgg gctccaggcc cgtctgctgg gcgaccatca gggtcagcag ggcgtaggag
     5461 gcgatgttga agggcacgcc gaggaacagg tcggcgctgc gctggtagag ctggcaggag
     5521 agcttcccgt cggcgacgta gaactggaac atggcgtggc agggcgccag ggccatgtcg
     5581 gggatgtcgg ccgggttcca ggcggagacg atcaggcggc gcgagtccgg cgtcgccctg
     5641 atctgctcga tcacctggga gagctggtcg atgctctcac cgtccggcgt cggccacgaa
     5701 cgccactgca cgccgtacac ggggccgaga tcgccgtccg catccgccca ttcgtcccag
     5761 atcgagacgc cgtgctcctg cagccagcgc acgttcgact cgccgcgcag gaaccacagc
     5821 agctcgtacg cgatcgattt gaagtgcacg cgcttggtcg tgatcagcgg gaagccctgg
     5881 gacagatcga agcggatctg cctcccgaac acactggtcg tccccgtgcc ggtgcgatcg
     5941 tccttgtgcg tgccgtgctc gagcacgtcg cggagcaggt cttcataggg cgtggggacg
     6001 gcggcgctca tgcctgccac gatacccgcc ggggcggggt cggaggcgtt ccgtcatgat
     6061 ccgtcacatc gggtcgtgca tccagcggac ggacttatcc tcggctcatg tccccgatcc
     6121 ccgcgctgct cgtcctggcg gcgctcgtcg tcatcgccgc cgtggccggc gtcgtctggc
     6181 gcgcccgcga cggccgtcgt cgccgtggat ccgatgtcgt cgacctcacc gacctgggag
     6241 tcactcccgg gcgcgtcacg ctggtgcagt tcggcaccga gacgtgcgcc cgctgcccgc
     6301 aggtgcgacg gatgctgcag ggcatcgcat ccgctctgcc gaccgtcgac cacgtcgacg
     6361 tggacctgac gcaccgcgcc gatctggccc gggcgcaccg tgtgctcggc acaccgacga
     6421 cgttcctcat cggatcggac gccgttctgg tcgcgcgttt caacggcgtg ccgcgccgcg
     6481 cggatgtcga ggccgcactc agcgaactgc ccgtgctcca ggaggcatca tgaccgagac
     6541 cgctcccgcc actccgcgcg gcatcgatcc gcgcgcgccg cgcttcgccg cctggatcac
     6601 ctccgtgctg ctgctggtgg cgaccttcct gggcctgatc gggatcggcg ggtcgcgctt
     6661 cgatcccgcg ttcgtgctga cggtcgtgat cgcgctgctg ttcctgtggg gcgtgctctc
     6721 ccccacgacg tctccgtgga gcgtgctgtt ccgccgcgtg gtgcggccgc gcctcgcgcc
     6781 gccgaccgac ctcgaggatc cccgaccgcc gcgcttcgcg caggg
//
LOCUS       DATMSX010000490        10540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_8041,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000490 DATMSX010000000
VERSION     DATMSX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8041"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6167
                     /locus_tag="VN041_15580"
     CDS             <1..6167
                     /locus_tag="VN041_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWU30503.1"
                     /translation="LAPAQDGSWRFDFGTASSPVADGYQQVLTSSLYTADAGWGVTTA
                     DGVSLFDRDRTGNRTPADPVAEDFVAGTDWGFLLDGVPNGEYDVTVSIGDALAGTSGT
                     NATVSLEGVAQTRMTTDKAATTTQTFRTAVEDGQLTLGFTGSGLGAYVNGLVVTPVVP
                     GVPADLTLARVAWNAVDLSWAAADGASAYRVLRAPVTGDAVGDFQQVAETDRTAFTDS
                     DVTEGDSFAYAVQAVNSYGVTSARTEPVRSGVIPALAAPTTPADLTVTKVSKTSVSLS
                     WSAATNAEEYVVERAGADGVFAAIATTASVSYLDEADTTASWSYRVTARNAAGSSEAS
                     APVVSPVYTAPAPLPAGDQVVFDFGPGAVADGALGVTSTTTYSDEWGYGFSTAPTSDA
                     PDVDRGGDDPLRSDHVAAQGGTFEVKLGAGDYTVNLLAGDATAASEISVTAEGMAKVQ
                     TTAKPAGEYLEMAFPIALVDGVLSLKVDGSAAALDALTITRQQARLAGALPTVYIAGD
                     STVQTYDAAAYAPQAGWGQMIDRFFGDGVAFSNHAIGGRSSKNFITQGRLDDILRGIR
                     PGDYLFVQFGHNDATQGVDDRYASPADYKEYLRTYVHGARQRGATPVLVTPVSRRSFD
                     ATTGLANISFPEYVDKMKELAAEEGVELVDLSASSRAYLNEIGAEAAKSVFLWVDPGI
                     FPNRPSGTQDDTHFQEYGAIQMARLIAQDYAEIDDPLAAKVEDIAPPAAVPPAPERLV
                     AGSVSNAGAVLQWQPAPTADIYRIERQAVADPDQVWSLVTTTTQTSAIVQGLAEGTGY
                     RYRVFAVNGRGTSEASNTVTFTTKKAKWKFDFQLAGNPLMAGYTEVNPDDAYSKEAGY
                     GFATALPATAGRDRGAAEGSNDLLRDFVLPGDSSEFTLDVPNGTYSVKTYSGDWIGST
                     KTSFDIEGKAFGSGNAGKGSVNESVRGPALVTDGQLNIRVYGAAAGTRLNGLEITPIL
                     LGPAGLKVADINADPTAPAVQLEWTTDSDVTWNVYRTSEFEKKATKVGETTTASFTDT
                     TARIGLDYTYYVTAVDQTGLESVPSASVPVSFVDPDVATPSAPTGLAVDRLEKREVEL
                     SWEQPSTALYAMVFRSEVAGERGELVGFASTNHFVDNSVLTTIPYHYTVVAVNAGGAG
                     KASAQLTTDAATVLQRQAEYLDRAPVAVSTADGTLVSWRMLGTDPDAIAFHVYRDGKR
                     ITDEPITSSTNYLDKKGSTDSQYFVTSVLDGREVTVTKTFGVQKGDYLSVPLDRPADT
                     YSKDGQPITYRANDASVGDVDGDGQYELFVKWDPSRSKDNSQSGYTGNVFLDAYRLDG
                     TRLWRVDLGANIRAGAHYTQFQVFDYDGNGRAEVIMKTADGTIDGTGQVIGNASADYR
                     NSSGYVLTGPEYLTVFDGATGRAIDTIDYVPARGDVGAWGDTYGNRVDRFLAATAYLD
                     GEHPSAIFSRGYYTRAVMAAYDFDGSQLVQRWVLDSNDPGNEGFYGMGNHNLSVADVD
                     GDQKDEIVYGSATVDDDGTLLYSTGLGHGDALHVSDLIPSRPGLEVFAAHEEMHASGN
                     RGATMRDARTGEVLWSIPATKDTGRAAAADIDPRHEGAEGWAVGGDAAWNSPVGQLKS
                     ATGELISENIPAANFLAWFDGDPLREIVDHDFDTDTRIGVPTVSKWNWETEKSDVILK
                     ADGARTNNDTKGTPNLQADLFGDWREEIAWRSDDSSELRIYSTTDATDLRLRTLMHDP
                     VYRLSVAWQNTGYNQPPQTSFFLGEGMEQPAAPRIAVTGDHTGATDTTAPVLAGLPAD
                     GALLASSASFTVGVTASDPESGVRNLDIAFDGEPVAPGQVLPLDGLVGPHTLTVNAVN
                     HDGLVTRASVSLLVFEDEGAKSAPGRGVLSTDSGWDTGLHDGDYTVSMNLWSGVNGAV
                     FKLYENGVLISTTLLDANSPKAQLTTVAVSGKPDGTYVYTGELINAKGSTATTSVTVK
                     VKDAAPGVPVVSHDNGDRNGDYTVSADLWWGTNATSYRLYENGKVIDEQSLVAASPNA
                     QHASTAVTGRARGTYEYVAEFINAAGATTSKAVTVTVR"
     gene            6286..6636
                     /locus_tag="VN041_15585"
     CDS             6286..6636
                     /locus_tag="VN041_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HWU30504.1"
                     /translation="MTRVGFQLQVRPELLDEYLARHSPVWPEMLAEIAAAGRRDYSLF
                     LAPGGVLFGYYETDDDAAAQAYLAASEVAARWEAEMARFFVGLEGRPDQAATPLTEVF
                     HLETQLAAAESTES"
     gene            6633..7796
                     /gene="rhaI"
                     /locus_tag="VN041_15590"
     CDS             6633..7796
                     /gene="rhaI"
                     /locus_tag="VN041_15590"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HWU30505.1"
                     /translation="MSILTPEILSELEQQSIELPSWAFGNSGTRFRVFGTPGTPRDPF
                     EKIADAAQVHKHTALAPTVALHIPWDTCDFGDLRKHAEDLGVKLGTVNSNTFQDEDYK
                     FGALTHEDAAVRRKAIDHHLACIDVMDATGSHDLKIWLAEGSNYPGQADMRGRQDRLN
                     ESLREIYSRLGDDQRLVLEYKFFEPAFYHTDVPDWGTSYAQVSTLGEKAMVCLDTGHH
                     APGTNIEFIVMQLLRLGKLGSFDFNSRFYADDDLIVGAADPFQLFRILFEVVRGGGLN
                     NPDVAFMLDQCHNIEDKIPGQIRSVLNVQEMTARALLVDREALAAAQKTGDVLGANAV
                     FMDAFYTDVRPALAEWRESRGLPADPMAAFAASGYLERIAADRVGGVQAGWGA"
     gene            7805..9094
                     /locus_tag="VN041_15595"
     CDS             7805..9094
                     /locus_tag="VN041_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010525855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30506.1"
                     /translation="MQKRTLTAWKATIAVAMSNYIEAGAIIALATSLTLWQGEFGFDD
                     GAVGLVAALSANAFGAAIGAAIGGPLCDRLGRKFIYTYDLIVFMIGALMVTFSPNFAV
                     LLIGVVLMGIAVGAGVPASWTYIAEEAPSEQRAAHVGTAQLAWSIGPAIGFLLAVVLG
                     PLGIVGSRIIFFHLFVIAAVTWWVRRGLPESARWKAQRETAQEQGEKVSLFSGIRGLL
                     SDRRNLGALLFLLGVYGLWNTVAGQAGIFQPRVYDAAGLHDATQQNLLQVLVWTLTAA
                     ATYFGFMRFGDRVSRRWLYFGGALLGVIAWVVLVFAPPGLASLLFFAIAWGISAGIGA
                     QAFYGLWTAELFATKYRASAQGMLFMLARVMVGLLSLVFPVLLTSWGLPQLGMLIIGL
                     LIAALLIGTIWAPRTEGKSLEQIERERYGERVDADLV"
     gene            9128..>10540
                     /gene="rhaD"
                     /locus_tag="VN041_15600"
     CDS             9128..>10540
                     /gene="rhaD"
                     /locus_tag="VN041_15600"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HWU30507.1"
                     /translation="MTNPTAAALIARSNRLGADPKNTNYAGGNTSAKGTETDPVTGQP
                     VELLWVKGSGGDLGTLTEQGLAVLRLDRMRSLVNVYPGIDREDEMVAAFDYCLHGKGG
                     AAPSIDTAMHGLVDAAHVDHLHPDSGIAIATAADGPELTAKIFGDKVVWVPWRRPGFQ
                     LGLDIADIKAKNPQAVGCILGGHGITAWGDTSEESEKNSLWIIDTAAAYIASHGKEQP
                     FGGIRPGFEALPDAERRARAAALAATVRGIASTDKPMVGHFTDAPVVTDFLASEKAPA
                     LAALGTSCPDHFLRTKVKPLILDLPATASVDEQIARLHELHAEYRADYQAYYDAHATA
                     DSAGASGAWPAIRGADPLIVLIPGVGMFSYGANKQTARVAGEFYVNAINVMRGAEALS
                     TYSPISDAEKFRIEYWALEEAKLQRMPKPKSHQGRIAFVTGAASGIGKAIATRLAAEG
                     ACVVVADLDLEKAQAAAAELG"
BASE COUNT         1737 a         3788 c         3504 g         1511 t
ORIGIN      
        1 cgctcgcgcc cgcgcaggac ggcagctggc ggttcgactt cggcacagca tccagtcccg
       61 tcgccgacgg ctaccagcag gtgctcacga gctcgctgta cacggccgac gccggctggg
      121 gcgtgaccac ggcggatggc gtcagcctct tcgaccgcga ccgcaccggc aaccgcaccc
      181 ccgccgaccc cgtcgccgag gacttcgtcg ccggcaccga ctggggcttc ctgctcgacg
      241 gcgtgccgaa tggcgagtac gacgtcaccg tctcgatcgg cgacgctctc gccggcacct
      301 ccggcacgaa cgccacggtg tccctggaag gggtcgcgca gacccgcatg accacggaca
      361 aggccgccac gaccacccag acgttccgca ccgccgtcga ggacgggcag ctgaccctcg
      421 gcttcacggg cagcggactc ggcgcctacg tgaacggcct cgtcgtcact ccggtcgtgc
      481 ctggagtgcc cgccgacctc accctcgccc gcgtcgcctg gaacgccgtc gacctcagct
      541 gggccgccgc tgacggggca tccgcatatc gcgtgctccg cgcacccgtc accggcgacg
      601 ccgtcggcga cttccagcag gtcgccgaga ccgacaggac cgccttcacc gactcggacg
      661 tcaccgaggg cgactccttc gcctacgccg tccaggccgt gaactcctat ggcgtcacct
      721 ccgcacggac cgagccggtg cgcagcggcg tcatccccgc tctggcagcg cccaccaccc
      781 ccgccgatct gaccgtgacg aaggtctcca agacctccgt cagcctcagc tggagcgccg
      841 cgacgaacgc cgaggagtac gtcgtcgagc gtgcgggtgc cgatggcgtc ttcgcggcca
      901 tcgccaccac cgcgtcggtg tcgtacctcg acgaggcgga caccaccgcg tcgtggtcct
      961 accgcgtcac cgcgcgaaac gcggccggct cgtccgaggc atccgctcca gtcgtctcgc
     1021 ccgtctacac cgcgcccgcg cccctccctg caggcgacca ggtcgtgttc gacttcggtc
     1081 ccggcgcggt ggccgacggc gccctcggcg tcacatcgac gaccacatac tccgacgagt
     1141 ggggctacgg cttcagcacc gcgccgacct cggatgcccc ggatgtggac cgcggcgggg
     1201 acgacccgct gcgcagcgac cacgtcgcgg cgcagggcgg caccttcgag gtcaagctcg
     1261 gagccggtga ctacacagtg aacctcctcg ccggcgacgc gacggcggcc agcgagatct
     1321 ccgtcaccgc cgaggggatg gcgaaggtgc agaccaccgc gaagcccgcc ggcgagtacc
     1381 tcgagatggc gttcccgatc gcactggtcg acggcgtgct ctcgctcaag gtcgacggtt
     1441 cggccgccgc tctcgacgct ctcacgatca cccgtcagca ggcgcgcctc gccggtgcgc
     1501 tgcccaccgt gtacatcgcg ggcgactcga cggtgcagac ctacgacgcc gcggcgtacg
     1561 caccacaggc cgggtggggc cagatgatcg accggttctt cggcgacggc gtcgccttca
     1621 gcaaccacgc gatcggaggc cgcagctcga agaacttcat cacgcagggc cggctcgacg
     1681 acatcctgcg cggcatccgg cccggcgact acctgttcgt ccagttcggg cacaacgacg
     1741 ccacccaggg cgtcgacgac cgctacgcat cgccggccga ctacaaggag tacctgcgca
     1801 cctacgtgca cggcgcgcgc cagcgaggcg ccacgccggt gctggtgacc ccggtctccc
     1861 gccgcagctt cgacgccacc acgggcctcg ccaacatcag cttccccgag tacgtcgaca
     1921 agatgaagga gctggcggcc gaggagggcg tggagctcgt cgatctgtcg gcttccagcc
     1981 gcgcctacct gaacgagatc ggcgcggagg ccgccaagtc ggtgttcctg tgggtcgacc
     2041 cgggcatctt cccgaaccgt ccgtccggca cgcaggacga cacccacttc caggagtacg
     2101 gcgccatcca gatggcccgc ctgatcgccc aggactacgc cgagatcgac gacccgctcg
     2161 ctgcgaaggt cgaggacatc gcacctccgg ccgccgtgcc gcccgcaccc gagcgactcg
     2221 tcgccggctc ggtgtccaac gcgggagcgg tgctgcagtg gcagccggcc ccgaccgccg
     2281 acatctaccg gatcgagcgt caggcggtcg ccgaccccga ccaggtctgg tcgctggtga
     2341 ccacgacgac gcagaccagt gccatcgtgc aggggctcgc cgagggcacc ggctaccgct
     2401 accgggtgtt cgccgtcaac ggccgcggca cctccgaggc atcgaacacg gtcaccttca
     2461 ccaccaagaa ggcgaagtgg aagttcgact tccagctcgc aggcaacccg ctgatggccg
     2521 gttacaccga ggtgaacccg gacgacgcgt acagcaagga ggccggatac ggcttcgcga
     2581 cggcgcttcc cgcgacggcc ggccgtgatc gcggcgccgc cgagggctcg aacgatctcc
     2641 tgcgtgactt cgtgctcccc ggcgactcga gcgagttcac gctcgacgtg ccgaacggca
     2701 cctactcggt caagacgtac tcgggcgact ggatcggatc cacgaagacg agcttcgaca
     2761 tcgagggcaa ggccttcgga tccggcaatg ccgggaaggg ctccgtcaac gagtccgtcc
     2821 gcggccccgc gcttgtcacc gacgggcagt tgaacatccg cgtctacggc gccgcagcgg
     2881 gtacccgcct gaacggtctg gagatcacgc cgatcctgct cgggccggcc ggcctgaagg
     2941 tcgccgacat caacgcagac ccgaccgcac cggcggtgca gctggagtgg acgaccgatt
     3001 ccgacgtcac gtggaacgtc tatcgcacct cggagttcga gaagaaggcg accaaggtcg
     3061 gcgagaccac gacggcgtcg ttcaccgaca ccacggcgcg catcggcctg gactacacgt
     3121 actacgtcac cgcggtcgac cagaccggac tggagtcggt gccctcggca tccgtccccg
     3181 tctcgttcgt cgaccccgac gtcgccaccc cgtccgctcc gaccgggctc gccgtggatc
     3241 gcctcgagaa gcgcgaggtc gagctctcct gggagcagcc gagcaccgcg ctgtacgcga
     3301 tggtgttccg ctccgaggtc gccggcgagc gcggtgagct ggtcggtttc gcctcgacca
     3361 accacttcgt cgacaacagc gtcctcacca ccatcccgta ccactacacg gtggtcgcgg
     3421 tgaacgcggg cggtgcagga aaggcatccg ctcagctgac gacagatgcg gccaccgtgc
     3481 tgcagcgcca ggccgagtac ctggatcgcg cgccggtcgc ggtgagcacc gccgacggca
     3541 cgctggtgag ctggcggatg ttggggaccg accccgacgc gatcgccttc cacgtctacc
     3601 gggacggcaa gcgcatcacc gacgagccga tcacgagctc gacgaactac ctcgacaaga
     3661 agggctcgac ggactcgcag tacttcgtca cgagcgtgct ggatggccgc gaggtcaccg
     3721 tcaccaagac gttcggcgtg cagaagggcg actacctctc cgtgccgctg gaccggcccg
     3781 ccgacacgta ctcgaaggac ggccagccga tcacgtaccg cgcgaacgac gcgagcgtgg
     3841 gcgacgtgga cggcgacggg cagtatgagc tgttcgtgaa gtgggacccc tcccgctcca
     3901 aggacaactc gcagtccggt tacaccggca atgtcttcct ggatgcctac cgcctcgacg
     3961 ggacccggct gtggcgggtc gatcttgggg cgaacatccg ggccggcgcg cactacacgc
     4021 agttccaggt gttcgactac gacggcaacg gccgcgccga ggtgatcatg aagaccgccg
     4081 acggcacgat cgacggcacg ggccaggtga tcggcaacgc cagcgccgac taccgcaaca
     4141 gctcgggcta cgtgctcacc gggccggagt acctgacggt gttcgacggt gcgacaggca
     4201 gggcgatcga cacgatcgac tacgtgccgg cgcgcggcga cgtcggcgcc tggggtgaca
     4261 cctacggaaa cagggtcgac cgcttcctgg cggcgaccgc gtacctcgac ggcgagcacc
     4321 cgagcgcgat cttcagccgc gggtactaca cccgcgcggt gatggcggcg tacgacttcg
     4381 acggctccca gctcgtgcag cggtgggtcc tcgactcgaa cgaccccggc aacgaggggt
     4441 tctacgggat gggcaaccac aacctgtcgg tggccgacgt cgacggcgat cagaaggacg
     4501 agatcgtcta cggttcggcg acggtcgacg acgacggcac gctgctgtac tcgacgggcc
     4561 tcggccacgg cgacgcgctg cacgtgtccg acctgatccc gtcccgtccc ggcctggagg
     4621 tgttcgccgc gcacgaggag atgcacgcat ccggcaaccg cggtgcgacc atgcgcgacg
     4681 cccgcacggg cgaggtgctg tggtcgatcc cggccacgaa ggacaccggg cgcgcggccg
     4741 ccgcggacat cgacccgcgg cacgagggcg ctgagggctg ggccgtgggc ggcgacgccg
     4801 cgtggaactc cccggtcggt cagttgaagt cggccacggg cgagttgatc tccgagaaca
     4861 tcccggcggc gaacttcctc gcctggttcg acggcgaccc gctgcgggag atcgtcgacc
     4921 acgacttcga caccgacacc cgcatcggcg tgccgaccgt gtcgaagtgg aactgggaga
     4981 cggagaagtc cgacgtcatc ctgaaggcgg acggagcccg cacgaacaac gacacgaagg
     5041 gcaccccgaa cctgcaggcc gacctgttcg gcgactggcg cgaggagatc gcgtggcggt
     5101 cggatgactc cagcgagctg cggatctact cgacgacgga tgccacggat ctgcgcctgc
     5161 gcacgctgat gcacgacccg gtgtaccgcc tgtcggtcgc ctggcagaac accggctaca
     5221 accagccgcc gcagaccagc ttcttcctcg gcgaggggat ggagcagccc gcggcgccgc
     5281 ggatcgcggt gaccggtgac cacaccggcg cgaccgacac cacggccccc gtcctggccg
     5341 gcctcccggc ggacggtgcg ctgctggcca gctcggcgtc gttcacggtg ggggtcacgg
     5401 cctccgatcc ggagtccggc gtgcgcaacc tcgacatcgc gttcgacggc gagcccgtcg
     5461 caccgggtca ggtgctcccg ctcgacggcc tggtcggccc gcacacgctc acggtgaacg
     5521 ccgtcaatca cgacgggctg gtcaccaggg catccgtctc gctgctggtg ttcgaggacg
     5581 agggggccaa gtcggctcca ggtcggggcg tgctctccac ggactccggc tgggacaccg
     5641 ggctgcacga cggcgactac accgtgtcga tgaatctgtg gagcggcgtg aacggggcgg
     5701 tgttcaagct ctacgagaac ggcgtgctga tctcgaccac gctgctggac gcgaactcac
     5761 ccaaggcgca gctgaccacg gtcgcggtga gcggcaagcc cgacggcacc tacgtctaca
     5821 ccggcgagct gatcaacgcg aagggcagca cggcgacgac atcggtcacc gtgaaggtga
     5881 aggatgccgc gcccggcgtg cccgtggtga gccacgacaa cggagacagg aacggcgact
     5941 acaccgtgtc agccgatctg tggtggggta ccaacgccac ctcgtaccgg ctgtacgaga
     6001 acggcaaggt gatcgacgag cagagcctgg tcgcggcctc gccgaacgcc cagcacgcga
     6061 gcacggcggt caccggacgc gcccgcggga cgtatgagta tgtcgccgag ttcatcaacg
     6121 cggccggcgc cacgacgtcg aaggcggtga ccgtcacggt gcgctgagaa cgcaggaggt
     6181 actccggggt cggatcgctt gacagggcga tccgaccccg gatatgttta catgaaacga
     6241 ttcaagaaac atcggaccat cacatcgatg tgagaggacg tcaccatgac ccgagtcggg
     6301 ttccagctgc aggtgcggcc cgaactgctc gacgagtacc tcgcacggca ctccccggta
     6361 tggccggaga tgctggcgga gatcgccgcc gccggccgcc gcgactactc gctcttcctc
     6421 gcgccgggag gtgtgctgtt cgggtactac gagaccgacg acgatgccgc cgcgcaggcc
     6481 tacctcgcgg cgtcggaggt cgccgcccgg tgggaggcag agatggcgcg gttcttcgtc
     6541 ggcctcgaag ggcgtcccga ccaggcggcc acgccgctca ccgaagtctt ccacctcgag
     6601 acacagctcg ccgcagcaga aagcaccgaa tcatgagcat cctcaccccc gagatcctgt
     6661 ccgagctgga gcagcagtcg atcgagctcc cctcctgggc gttcggcaac tccggcaccc
     6721 ggttccgcgt gttcggcacc cccggcacgc cccgcgatcc cttcgagaag atcgcggatg
     6781 ccgcgcaggt gcacaagcac accgcgctgg cgcccaccgt ggctctgcac atcccctggg
     6841 acacgtgcga cttcggcgac ctgcgcaagc acgccgaaga cctcggcgtg aagctcggca
     6901 cggtcaactc gaacacgttc caggacgagg actacaagtt cggcgccctc acgcatgagg
     6961 acgccgcagt gcgccgcaag gccatcgacc accacctggc gtgcatcgac gtgatggacg
     7021 ccaccggcag ccatgatctc aagatctggc tggccgaggg ctcgaactat ccgggccagg
     7081 ccgacatgcg cggtcgccag gaccggctga acgagtcgct ccgcgagatc tactcccgcc
     7141 tcggcgacga tcagcggctc gtgctggagt acaagttctt cgagcccgcg ttctaccaca
     7201 ccgacgtccc cgactggggg acgagctacg cgcaggtgtc cacgctgggc gagaaggcga
     7261 tggtgtgcct ggacaccggt caccacgcgc ccggcacgaa catcgagttc atcgtcatgc
     7321 agctgctgcg gctgggaaag ctcggctcct tcgacttcaa ctcgcgcttc tacgccgacg
     7381 acgacctgat cgtcggtgcc gccgacccgt tccagctgtt ccgcatcctg ttcgaggtcg
     7441 tccgcggcgg cggcctgaac aaccccgacg tcgcgttcat gctcgaccag tgccacaaca
     7501 tcgaggacaa gatccccggg cagatccgct cggtgctgaa cgtgcaggag atgaccgccc
     7561 gcgcgctgct ggtcgaccgc gaggctctcg ccgcagcgca gaagaccggc gacgtgctcg
     7621 gcgccaatgc cgtgttcatg gacgccttct acacggacgt gcgcccggcg ctggccgagt
     7681 ggcgcgagtc gcgaggactg cctgcagacc cgatggcggc gttcgcggca tccggctacc
     7741 tggagcggat cgcggccgac cgggtcggcg gcgtgcaggc gggttggggg gcctgagcat
     7801 gagcatgcag aagcgaaccc tcactgcatg gaaggccacg atcgccgtgg ccatgtccaa
     7861 ctacatcgag gccggcgcga tcatcgcact ggccaccagc ctgacgctct ggcagggcga
     7921 gttcgggttc gacgacgggg ccgtcggcct ggtcgcggcg ctcagcgcga atgccttcgg
     7981 cgcggccatc ggcgcggcca tcggaggacc gctctgcgac cggctcggac gcaagttcat
     8041 ctacacgtac gacctgatcg tcttcatgat cggcgcgctg atggtgacct tctcgccgaa
     8101 cttcgccgtg ctgctgatcg gcgtcgtgct catgggcatc gccgtcgggg cgggcgtgcc
     8161 ggcgtcgtgg acctacatcg ccgaggaggc gccgagcgag cagcgcgcgg cgcacgtcgg
     8221 caccgcgcag ctggcctggt cgatcggccc ggccatcggc ttcctgctgg cggtcgtgct
     8281 cggcccgctc gggatcgtgg gctcccggat catcttcttc cacctgttcg tgatcgcggc
     8341 cgtgacctgg tgggtgcgcc gcggcctgcc cgagtccgcg cgctggaagg cgcagcgcga
     8401 gaccgcgcag gagcagggcg agaaggtgtc gctgttctcc ggcatccgcg ggctgctctc
     8461 ggaccgccgc aacctgggcg cgctgctgtt cctgctcggc gtgtacggac tctggaacac
     8521 ggtggccggc caggccggca tcttccagcc acgcgtctac gacgcggcgg gtctgcacga
     8581 cgcgacccag cagaacctgc tgcaggtgct ggtgtggacg ctcaccgcgg cggcgaccta
     8641 cttcggcttc atgcgcttcg gtgaccgcgt cagccgccgg tggctgtact tcggaggcgc
     8701 attgctgggc gtcatcgcct gggtcgtgct cgtcttcgcc cctcccgggc tcgcgtcgct
     8761 gctgttcttc gccatcgcgt ggggcatctc ggcgggcatc ggcgcgcagg ccttctacgg
     8821 cctgtggacc gcggagctgt tcgccacgaa gtaccgcgcg agcgcacagg ggatgctctt
     8881 catgctcgcc cgcgtcatgg tcggcttgct gagcctcgtc ttccccgtgc tgctcaccag
     8941 ctggggcctc cctcagctcg gcatgctgat catcggcctg ctgatcgcgg cgctgctgat
     9001 cggcaccatc tgggctcccc gaaccgaagg caagtctctc gagcagatcg aacgcgagcg
     9061 ctacggcgag cgcgtcgacg ccgatctcgt ctgacaccat ctccgaatcc acgaaggact
     9121 cctcctcatg acgaacccca ctgccgccgc tctcatcgcc cggtcgaacc ggctcggcgc
     9181 cgacccgaag aacaccaact acgccggcgg caacacgtcc gccaagggca ccgagaccga
     9241 ccccgtcacc gggcagcccg tcgagctgct gtgggtgaag ggctccggcg gcgacctcgg
     9301 cactctcacg gagcagggtc tggccgtgct gcgactggat cgcatgcgct cgctggtgaa
     9361 cgtctacccc ggcatcgacc gcgaggacga gatggtcgcc gcgttcgact actgcctgca
     9421 cggcaagggg ggagccgccc cgtcgatcga caccgcgatg cacggactcg tggatgccgc
     9481 acatgtcgac cacctgcacc ccgacagcgg catcgccatc gcgaccgccg ccgacggccc
     9541 ggaactgacg gcgaagatct tcggcgacaa ggtggtctgg gtgccgtggc ggcgccccgg
     9601 cttccagctg ggcctggaca tcgcggacat caaggcgaag aacccgcagg cggtcggctg
     9661 catcctgggc ggccacggca tcaccgcctg gggcgacacc tcggaggagt ccgagaagaa
     9721 ctccctgtgg atcatcgaca ccgccgccgc gtacatcgcg tcccacggca aggagcagcc
     9781 cttcggcggc atccgcccgg gcttcgaggc actgccggat gcagagcgcc gggcccgcgc
     9841 cgcggccctc gccgccaccg tccgcggcat cgcgtccacc gacaagccca tggtcgggca
     9901 cttcacggat gcccccgtcg tcaccgactt cctggcatcc gagaaggcgc ccgcgctggc
     9961 ggcgctcggc acaagctgcc ccgatcactt cctgcgcacc aaggtcaagc cgctgatcct
    10021 cgacctgccg gcgaccgcgt cggtcgacga gcagatcgcc cggctgcacg agctgcacgc
    10081 cgagtaccgc gccgactacc aggcgtacta cgacgcgcac gccaccgcgg acagtgctgg
    10141 tgcctcgggc gcctggcccg cgatccgcgg cgccgacccg ctgatcgtgc tgatccccgg
    10201 cgtgggcatg ttctcctacg gggcgaacaa gcagaccgca cgcgtcgccg gggagttcta
    10261 cgtcaacgcc atcaacgtca tgcgcggtgc ggaggcgctc tccacctact cccccatctc
    10321 ggatgccgag aagttccgca tcgagtactg ggcgctggaa gaggcgaagc tgcagcggat
    10381 gccgaagccg aagagccacc aggggcgcat cgcgttcgtc acgggcgcgg catccggcat
    10441 cggcaaggcg atcgcgaccc gtctcgccgc cgagggcgcg tgcgtggtgg tcgccgacct
    10501 cgacctggag aaggcgcagg ccgccgccgc cgagctcggt
//
LOCUS       DATMSX010000491         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_159265,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000491 DATMSX010000000
VERSION     DATMSX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159265"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..120)
                     /locus_tag="VN041_15605"
                     /pseudo
     CDS             complement(37..120)
                     /locus_tag="VN041_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
     gene            632..775
                     /locus_tag="VN041_15610"
     CDS             632..775
                     /locus_tag="VN041_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30508.1"
                     /translation="MPRCPDDGILMRDIPGGWVCPHCGHVQQAQDVEMPPEFDGPDLD
                     QRR"
     gene            796..921
                     /locus_tag="VN041_15615"
     CDS             796..921
                     /locus_tag="VN041_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30509.1"
                     /translation="MPAHTISPDTMLSIRVGCVMTRNQFTQNPARRDDRTSRDGR"
     gene            complement(1001..1333)
                     /locus_tag="VN041_15620"
     CDS             complement(1001..1333)
                     /locus_tag="VN041_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30510.1"
                     /translation="MKALFDSLVRTYVPWFVGIAVGWLVSLGIPLDPDVEPQITAALM
                     LVSSMLWYFLARVFETYVHPKLGWLIGLPKQPLYDGKSRDQILADFARLDPDEQNADL
                     QAALARRR"
     gene            complement(1317..1772)
                     /locus_tag="VN041_15625"
     CDS             complement(1317..1772)
                     /locus_tag="VN041_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30511.1"
                     /translation="MPPEIQEFFEGLSVWTVIVWLVAIGISFPILRKLWRLFKQLADF
                     LDDVMGEPARPGVEARPGLMERVMRVEHELFPNSGRSLRDQTNRMEEKLDRDNARITE
                     LAGQVEDVVGKVDDVAVNVGGLTERVDEHITQSTQIIETLKGDNHEGPV"
     gene            complement(1777..1914)
                     /locus_tag="VN041_15630"
     CDS             complement(1777..1914)
                     /locus_tag="VN041_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30512.1"
                     /translation="MKRGTNIQHGRRRRYPPSMLIPTAIIVVCYPVLIWLLIEMFKAV
                     I"
     gene            complement(1911..>2291)
                     /locus_tag="VN041_15635"
     CDS             complement(1911..>2291)
                     /locus_tag="VN041_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30513.1"
                     /translation="PAPRPSTPGQLDVDGDLGVATIRRLQEVLGTPVDGKISTPKSTV
                     VAELQRRLNAAGARDWDGKPLVVDGVGLRSNHLARVPLVGRWRTIWALQAHLGTTKDG
                     VLNRRSSEVVRELQRRLNAGTLGL"
BASE COUNT          365 a          802 c          765 g          359 t
ORIGIN      
        1 ccggcgcccg cgcgagacgc gcccggtgcg cgcgcatcac gacaccgtca gcgcccgccg
       61 cacgaggtcg gtgaggatgc cgcgcacctc gtccgacctc atgatcaccg tcatccgacc
      121 atttcagacg aaccgcttcg ccctacgcgt cccactcccc tatgtcgaag ccgtcctcgc
      181 cgggcccgcc ttcgggctgc atccaggagt catcgacctc gcggcggagc acgcaggtga
      241 gctggtcgtc gggaggcatc cggtgatcga ggacgacgtc gatcatgtcg cccgcggtga
      301 gtgggtgctc ggtcacgagc tcgcggccgt ccttgcgcgt catgctgccg ccctcccccg
      361 ccggccgacc atcgagcgga cacgtctcgc cgtgatcgga tccgcggcca tcccctcgat
      421 cgaccgcgcc gcccgcatca gcatctggta gaaccgagcc ggcgggatgc cgagctcggc
      481 gatgatcgcg gcttccttgc cgggcggatg gcctgggtgt cgtcgctcga acgcgagaag
      541 ggcagatggc gtcatggcct ctaccctgcc gacgaccacc gacatacgct gtcgggatgg
      601 acgacgatct cgactggggc gcgcagcccc gatgccccga tgccccgacg atggcatcct
      661 catgcgcgac atccccggcg ggtgggtgtg cccgcactgc ggccacgtcc agcaggctca
      721 ggacgtggag atgccgccgg agttcgacgg gccggatctc gaccagcgtc ggtgacacgg
      781 cgtaccgtcc agcgcatgcc cgcacacacc atctcccccg acacgatgct cagcatccgc
      841 gtcggctgcg tcatgacccg caaccagttc acacagaacc cggcccgccg tgatgaccga
      901 acttcgcgag acggccggtg accgcctcga catcgccgac tgactcaacg acgaaaccgc
      961 ccctcgctat cccgaaggat gcgaggggcg gttcagcgtg tcagcggcgt cgcgcgagag
     1021 cagcctgcaa gtcggcgttc tgctcgtcgg ggtccaggcg cgcgaagtcc gcgaggatct
     1081 gatctcggga cttgccatcg tagagcggct gcttcgggag gccgatcagc cagccgagct
     1141 tcgggtgcac gtacgtctcg aacaccctgg cgaggaagta ccagagcatc gaggacacga
     1201 gcatcagcgc cgcggtgatc tgcggttcga cgtcggggtc gagcgggatg ccgagcgaca
     1261 cgagccagcc gacggcgatg ccgacgaacc agggcacgta ggtgcgcacg agcgaatcaa
     1321 acagggcctt catggttgtc ccccttcagg gtttcgatga tctgtgtgga ctgggtgatg
     1381 tgctcgtcga cgcgctcggt cagcccgccg acattcacgg ccacgtcgtc gaccttcccg
     1441 acgacgtcct cgacctgccc ggcgagctcc gtgatgcgag cgttgtcgcg gtcgagcttc
     1501 tcctccatgc ggttcgtctg gtcgcggagt gaccggccac tgttggggaa cagctcgtgc
     1561 tcgacccgca tgacgcgctc catcaggccc gggcgggctt cgactccggg acgggcgggt
     1621 tcacccatca cgtcgtcgag gaagtcggcg agctgcttga acagccgcca cagcttccgc
     1681 aggatcggga agctgatgcc gatcgcgacg agccagacga tcaccgtcca cacggacagc
     1741 ccttcgaaga actcctggat ctcgggaggc atcgggtcag atcaccgcct tgaacatctc
     1801 gatcagcaac cagatcagga ccgggtagca gaccacgatg atcgccgtgg ggatgagcat
     1861 gctcggcggg tatcgccgcc ggcgtccgtg ctggatgttc gtgccgcgct tcacagcccg
     1921 agcgtccctg cgttcaggcg gcgctgcagc tccctgacca cctcggaact gcgccggttg
     1981 aggacgccgt ccttggtggt gccgaggtgc gcctgaagcg cccagatcgt gcgccaccgt
     2041 ccgacgagcg gcacccgtgc gaggtggttc gagcggagcc cgacgccgtc gacgacgagc
     2101 ggcttgccgt cccagtcgcg ggcgccggcc gcgttcaggc ggcgctgcag ctcggcgacg
     2161 accgtcgact tcggagtcga gatcttcccg tcgacagggg tcccgaggac ctcctgcagg
     2221 cggcggatcg tcgcgacccc gaggtcgccg tcgacgtcca gctgcccggg cgtcgacggt
     2281 cgcggggccg g
//
LOCUS       DATMSX010000492         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_187528,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000492 DATMSX010000000
VERSION     DATMSX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187528"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VN041_15640"
     CDS             <1..824
                     /locus_tag="VN041_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30514.1"
                     /translation="MTTLHSAAEATPAERPSNPTPADAAAALLAANRRRRSIRRWVAI
                     GTLPLTLAALLFVGKLLSMYAFAHQSISSFVTGGYAESESAARGQDFLNWFEQFKAPY
                     NIGTALAGADKLPQARTELERALPLAQGLDVCYVRINLSLVVERQGDAAQAKGDAAKA
                     AQFYGEALQITAETPKECSSDEAQNQSPDQNRDMKDTLDQNQQRQQEKQQQSQQQKPD
                     QDPQPQPEESEQPQDQPSQSDLDEIEKKLNEGAQDRENQTGGDQEGSGGGTDKPW"
     gene            818..1933
                     /locus_tag="VN041_15645"
     CDS             818..1933
                     /locus_tag="VN041_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30515.1"
                     /translation="MVEQQRSRYTGGAEGAPPLPPPGGYQGARRTSTGLDAAAGLAPA
                     SGDVVYTAAPPAEKKGVNALGLVAITATGLFVLVMLLMMGAGGTDALYGASMIVVQFV
                     VLGVIVAALFTVRGRTLGVIALAVTLVFNVATVGAMGALRSAATHAYGGTKSDKQKHE
                     EAYPGIKGVPERQALDNPSLEDVQAQGDQVMAEVRDRLTQQFGFTWVQTGKVDVSPER
                     NGYGGESMLQKYTSTVWTTEQPVQDTRRKRDVMRVVNQVLAENGMSDLYALNEPGVMG
                     ISNDMLTKLYGSDDPERQHTWEYYSDTYPDPLRFYTYVYDLSKDQTGDFRTQREAFKQ
                     QSGEPLEGVQLVVFARQVLQEKDRAAYEQKLKDYPGF"
     gene            2084..>2987
                     /locus_tag="VN041_15650"
     CDS             2084..>2987
                     /locus_tag="VN041_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HWU30516.1"
                     /translation="MTEVVVDKTEQRGSEQRAVERMYAHLDAEKSALEAAQARVAASP
                     AEGPESRLARDAELARLSGELGRLRLAERSLCFGRIDSSAGASMHVGRVGLRTESGEI
                     LLVDWRAEAARPFYAATPASPSGLRRRRHLRLDGRLVVGVSDEILDGTDPTSEDVVGD
                     GPIVSALSGARTGRMREAASTLQREQDEIVRSEHRGVMVVDGGPGTGKTIVALHRAAY
                     VLYAFPSVAERGVLVFGPNRRFLDYISDVLPSLGENDVDLATTADLVAVGATRCEPDA
                     VARAKGRSAFAEAIARKVRERQPFG"
BASE COUNT          507 a          971 c         1098 g          411 t
ORIGIN      
        1 gcatgaccac cctccactcg gcagccgagg ccactcccgc cgagcggccc tcgaacccga
       61 cgcccgccga cgccgccgcg gcgctcctgg ccgcgaaccg ccgcaggcgc agcatccgcc
      121 gctgggtcgc gatcggcacc ctcccgctca cgctcgccgc gctgctgttc gtcggcaagc
      181 tgctgagcat gtacgcgttc gcgcaccagt cgatcagctc gttcgtcacc gggggatacg
      241 cggagtcgga gtcggccgca cgcgggcagg acttcctgaa ctggttcgag cagttcaagg
      301 cgccgtacaa catcggcacg gcgctggccg gcgccgataa gctgccccag gcccgcaccg
      361 agctcgagcg cgcactgccc ctcgctcagg ggctggacgt ctgctacgtg cgcatcaacc
      421 tgtcgctggt ggtggagcgg cagggcgacg ccgctcaggc gaagggcgac gccgcgaagg
      481 ccgcccagtt ctacggcgag gcgctgcaga tcacggcgga gactccgaaa gagtgcagca
      541 gcgacgaggc gcagaaccag tcgcccgatc agaaccgcga catgaaggac acgctggatc
      601 agaaccagca gcgtcagcag gagaagcagc agcagagcca gcagcagaag ccggatcagg
      661 acccgcagcc gcagcccgag gagagcgagc agccgcagga ccagccctcg cagagcgacc
      721 tcgacgagat cgagaagaag ctcaacgagg gcgcccagga ccgcgagaac cagaccggcg
      781 gcgaccaaga gggctccggc ggcgggacgg acaagccgtg gtagagcagc agcgcagcag
      841 gtacacgggt ggcgccgagg gcgcgcctcc cctgccgccg cccggcggct atcagggcgc
      901 gcgtcgcacg tcgacggggc tggacgcggc agcgggtctc gcccctgcgt ccggcgacgt
      961 ggtctacacg gcggcacccc ccgccgagaa gaagggcgtg aacgcgctgg gcctggtcgc
     1021 gatcacggcg acggggctct tcgtgctggt catgctgctg atgatgggcg cgggcggaac
     1081 ggatgccctg tacggggcgt cgatgatcgt ggtgcagttc gtcgtgctcg gcgtgatcgt
     1141 cgccgcactg ttcaccgtgc gcgggcgcac gctgggcgtc atcgccctgg cggtgacgct
     1201 ggtcttcaac gtcgcgacgg tcggcgcgat gggcgcgctg cgctcggccg ccacgcacgc
     1261 ctacggcggc acgaagtcgg acaagcagaa gcacgaggag gcgtacccgg ggatcaaggg
     1321 cgtccccgag cgccaggcgc tggacaaccc gtcgctggag gatgtgcagg cgcagggcga
     1381 tcaggtgatg gccgaggtgc gcgaccgtct cacgcagcag ttcgggttca cctgggtgca
     1441 gaccggcaag gtcgatgtga gccctgagcg caacggctac ggcggcgagt cgatgctgca
     1501 gaagtacacc tccacggtgt ggacgaccga gcagcccgtg caggacaccc gccgcaagcg
     1561 cgacgtgatg cgggtcgtga accaggtgct cgccgagaac ggcatgtcgg atctgtacgc
     1621 gctcaacgag ccgggcgtga tgggcatctc gaacgacatg ctcaccaagc tctacgggtc
     1681 cgacgacccc gagcgtcagc acacctggga gtactactcc gacacctatc cggatccgtt
     1741 gcggttctac acctacgtgt acgacctgtc gaaggaccag accggggact tccgcaccca
     1801 gcgcgaggcc ttcaagcagc agagcggcga acccctcgag ggcgtgcagc tggtcgtctt
     1861 cgcccgccag gtgctgcagg agaaggaccg cgcggcgtac gagcagaagc tgaaggacta
     1921 ccccgggttc tgacagttgc gctcagcgcg tttccatgcc ctcggcgacg cccgacgctg
     1981 ggcgcggatg cgctggtcag gaggccaaat ccgggccgag aagtcaacgt tgtctttgcc
     2041 ggccgaaccc ttcaaggtgg acgctggacg aagggagtca cgggtgacgg aagtggtcgt
     2101 ggacaagacg gagcagcggg gatccgaaca gcgtgcggtg gagcggatgt atgcgcacct
     2161 ggatgcggag aagtccgccc tcgaggccgc acaggcgcgc gtggctgcgt cgccggccga
     2221 aggaccggag tctcgtctgg cgagggatgc cgaactcgca cgtctgagcg gggagctcgg
     2281 tcgtcttcgg ctggcggagc gctcgctgtg cttcggacgg atcgacagca gtgcaggagc
     2341 gtcgatgcat gtgggccgtg tcgggttgcg caccgagtcg ggtgagatcc tcctggtgga
     2401 ttggagggcg gaggccgctc gaccgttcta cgctgccacg ccggcgtctc cttcgggcct
     2461 gcgccgtcgt cgtcacctgc gtctcgatgg gcgcctggtc gtcggcgtct ccgacgagat
     2521 cctggacgga accgacccga ccagcgagga cgtggtgggc gatggaccga tcgtgtccgc
     2581 gctgagcggg gcgcggacgg ggcgcatgcg cgaggcggca tcgacgctgc agcgggagca
     2641 ggacgagatc gtgcgctcag agcatcgcgg cgtgatggtg gtcgatggcg gccccggaac
     2701 gggcaagacg atcgtcgccc tgcatcgggc cgcctacgtg ctctacgcgt ttccgtccgt
     2761 cgctgagcgg ggtgtgctcg tgttcgggcc caacaggcgt ttcctcgact acatctccga
     2821 cgtgctcccc tcgctcgggg agaacgatgt cgacctggcc acgacagcgg acctcgtcgc
     2881 ggtcggggcg acgaggtgcg agccggatgc ggtggcacgg gccaagggcc gcagcgcctt
     2941 cgcggaggcg atcgcgcgaa aggtgcggga gcggcagccg ttcggcg
//
LOCUS       DATMSX010000493         5659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_55047,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000493 DATMSX010000000
VERSION     DATMSX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55047"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..855)
                     /locus_tag="VN041_15655"
     CDS             complement(67..855)
                     /locus_tag="VN041_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU30517.1"
                     /translation="MRELWSTSGGAFIRYLDLPGDEVPIVFIHGHGCSGSHDYPQVAA
                     QPGLAGHRRVLVDLLGCGYSDRPDDFGYRVSDHAAVLEELIEHLNLNDFVLFGHSAGG
                     AVALELGRRVESRLRGLVLSEANLDPSPPDAGSYQVASQSEEAFIDRGYAQLISAARS
                     AGIDNWASAVTHWSPRAMWRLADSLKRGQQPSGRSVLYGLSVPRAYLIGENSLPDPDQ
                     SELPPRGVEVLIVPAVGHSMAWEDPAGVATAMAAAIEPWSPTPG"
     gene            complement(912..1526)
                     /locus_tag="VN041_15660"
     CDS             complement(912..1526)
                     /locus_tag="VN041_15660"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30518.1"
                     /translation="MTTERGYTTGRARREQIIAAATALFGRVGYNSATMLELSKICEI
                     SRAGLAHHFPTKESLMEAVLETRDREDRERFRRNGSQGQDGIGILRGMVDLARHNSGV
                     GGIIALYAVLSAEAADPTHPAHDYFVRRYARIRSGTAKALRDARAAGYLAEHVDEERA
                     AAELTALMDGLQLQWLLDPTSVDMAEAVRLRIEELLVVPLGGTD"
     gene            1654..2961
                     /locus_tag="VN041_15665"
     CDS             1654..2961
                     /locus_tag="VN041_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30519.1"
                     /translation="MSSTSSDAPELRPPITETTFVATASGEDDPPAPLKGVRRLLWWI
                     VPANFAIFVIWGAVPGILLQAQLIAMFPDEKTQVANIAIVMTIGALGAMIAQPAAGQL
                     SDRTRAKSGRRAVWIVGGAIVGGIALIVLGFMNSLIGIIVAWTLIQVAYNFAQGPLSA
                     VLPDRVPVARRGTFAALSGIGLMLGALGGQIVGSQLAHTITLGYVIFAAFAVVVLIGF
                     VIANPDHPSTNFQNEPFSFAEFLRTFWVNPVKHPDFFWAFTGRLLLYTGYFAVTGFQL
                     LILKNYLGVKDPFAVIPLLGILSLAGVLIATVISGPLSDKVGRRKPFVFGSSLLMAVA
                     LVVPWITPSYTSWMIMTVISGFGFGMFQAVDTALMSQVLPSAKNFAKDLGVVNIAATL
                     PQTFAPGVAGAIVLAFGYGALFPIAIVLSVLGAISVWFIKSVK"
     gene            2977..4737
                     /locus_tag="VN041_15670"
     CDS             2977..4737
                     /locus_tag="VN041_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU30520.1"
                     /translation="MTDTEVRFPYQDAALDIEQRVEDLLSRMDAADKAGMLFHTMVGI
                     GDPSEGNPTFGLPSAHSMIRKRRMNHFNLLGSAPSGREFAAWSNALQRFAREDGLGIP
                     ITFSTDPRHAFSDNPLTSMFAGPFSQWPETLGFAALASPERLERFSDIARREYLAVGL
                     RVALHPQIDLATEPRWSRINGTFGESADLTSELVVGYIRGFMGSELGPESVSTMVKHF
                     PGGGPQLDGEDPHFEWGKEQVYPGGEQEYHLRPFIAAIGAGATQMMPYYGKPVGTDWE
                     EVGFGFNKDVLTTLLRDRLGFDGIVCTDWGPLTDADILGTPMPARAWGVEHLTRPERM
                     VKALDAGIDQFGGELCTDVLLELLASGAVAEDRLDVSVRRLLREKFRLGLFDDPFVDE
                     EHAEAVVGRADFVQEGLDAQSDSLTLLTNTDAILPLTRDVKVYAEGVASDVLAGYATV
                     VETPAEADVAILRIKAPFEDRDRGGFSAMFHSGSLEFPAEEHARLTAIAEAVPTVIDI
                     YLDRPAVLGGLEESARAVLADYGASDEAVLRVLFGERGPQGSLPFDLPRSDAAVVASR
                     SDVAFDTEDPTFRFGHGLRY"
     gene            complement(4844..>5659)
                     /locus_tag="VN041_15675"
     CDS             complement(4844..>5659)
                     /locus_tag="VN041_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30521.1"
                     /translation="AAQAGSSAPAARAESILEVRGLGKTYGVRGGSFLAADDVSFAVP
                     RGKTLSIVGESGSGKTTSARIAARLVQPDAGTILLDGEDITRATGESLRQLRRRMQVV
                     YQNPFGSLDPRMTVERIVAEPLRSFRVGDRSSQRLLVRELLERVRLSSELADRRPGEL
                     SGGQRQRVAIARALAIGPELMILDEPVSALDVSVQEQILQLLVDLQAEFGLSYLFISH
                     DLGVVRQISDRIVVMQKGRVVESGDAADVLGAPAHEYTRELIAAIPGSSAVAG"
BASE COUNT          826 a         2055 c         1857 g          921 t
ORIGIN      
        1 gccctgcggg ccctggcgca cgccgcccgc tgagcgactc caggccctgg cgtggtcagt
       61 ggcgcgtcac ccgggcgtcg gactccacgg ctcgatcgcc gctgccatcg ccgtggccac
      121 accggcggga tcctcccatg ccatcgagtg gccgactgcc ggcacgatca gcacctcgac
      181 tccgcgcggc ggcagctccg actggtcggg gtcgggcagg gagttctcgc cgatgaggta
      241 cgcccgcgga acgctcaaac cgtacaggac gctccgaccg ctcggctgct gcccccgctt
      301 gagggaatcg gccagtcgcc acattgcgcg aggcgaccaa tgggtcactg ccgaggccca
      361 gttgtcgatg ccggcacttc gagctgctga gatcagctgt gcataaccgc gatcgatgaa
      421 cgcctcttcg gactggctgg cgacctgata cgagccggcg tccggcgggc tcgggtccag
      481 gttcgcctcg ctgagcacca ggccgcgcaa ccggctttcc actcgtcgac cgagctccaa
      541 tgcgaccgcg ccgcctgcgc tgtggccgaa gagcacgaag tcgttcagat tcaggtgctc
      601 gatcagttct tccagcacgg cggcatggtc gctcacgcgg tagccgaagt cgtcgggacg
      661 gtcgctgtaa ccgcatccga gcagatcgac caagactctc cgatgccccg ccaatcccgg
      721 ttgggcggcg acctgcggat agtcgtggct tcccgagcat ccatgcccgt ggatgaacac
      781 gatcggcacc tcgtcgcccg gtaggtcgag gtatctgatg aacgctccgc ccgaggtcga
      841 ccacagttca cgcacgcggc cagtattcca cggtcgccga ttcgccggac caggccattg
      901 ccagccccct gctagtcggt cccgcccagc ggcacgacga gcagctcctc gatccggagc
      961 cgcacggcct cggccatgtc gacggacgtc ggatcgagca gccactgcag ctgcagtccg
     1021 tccatgaggg cggtgagctc cgcggcggcg cgctcctcgt cgacgtgctc ggcgaggtag
     1081 cccgcggccc tggcatcccg cagggccttc gccgtgccgg agcggatccg cgcataacga
     1141 cggacgaagt agtcgtgcgc ggggtgggtt gggtcggccg cctccgccga cagcaccgcg
     1201 tacagcgcga tgatgccgcc gacgccgctg ttgtgtcggg cgaggtcgac catgccgcgc
     1261 aggatcccga taccgtcctg cccctgtgac ccgttgcggc ggaaccgctc gcggtcctcg
     1321 cggtcgcgcg tctccagcac cgcctccatg agggattcct tggtggggaa gtggtgcgcg
     1381 aggccggcgc gcgagatctc gcagatcttc gacagttcga gcatggtcgc actgttgtag
     1441 ccgacgcggc cgaagagcgc ggtcgcggcc gcgatgatct gctcccgccg ggctcggccg
     1501 gtggtgtatc cgcgttctgt cgtcatcgat gtcccatcgt aggcgtcaga tgaaaaccta
     1561 cacttgtgcc ggttttcatg ctagcgtgcc atcaatcgga accgagcacc ctgtccggcc
     1621 acccgaaccc gcccataccc gcaaggactg acaatgtcgt cgacttcatc tgacgccccc
     1681 gagctgcgtc ccccgatcac cgagaccacc ttcgtcgcca ccgcctccgg cgaggacgac
     1741 ccgcccgcgc ccctcaaggg cgtgcggcgc ctgctgtggt ggatcgtccc cgccaacttc
     1801 gcgatcttcg tgatctgggg cgcggtcccc ggcatcctgc tccaggccca gctgatcgcg
     1861 atgttccccg acgagaagac ccaggtcgcg aacatcgcca tcgtcatgac gatcggcgcc
     1921 ctcggcgcca tgatcgccca gcccgccgcg ggccagctct ccgaccgcac ccgggcgaag
     1981 tccggccgcc gcgcggtgtg gatcgtcggc ggagcgatcg tgggcggcat tgccctcatc
     2041 gtcctcggct tcatgaacag cctcatcggg atcatcgtcg cgtggaccct gatccaggtc
     2101 gcctacaact tcgcgcaggg cccgctctcc gcggtgcttc ccgaccgcgt gccggtcgcc
     2161 cgccgcggca ccttcgccgc cctgtccggc atcggactca tgctcggtgc gctgggcgga
     2221 cagatcgtcg gctcccagct cgcgcacacc atcaccctcg gctatgtgat cttcgcggcg
     2281 ttcgccgtcg tcgtgctgat cggcttcgtc atcgccaacc ccgaccaccc gagcaccaac
     2341 ttccagaacg agccgttcag cttcgcggag ttcctgcgca ccttctgggt caacccggtc
     2401 aagcaccccg acttcttctg ggcgttcacc ggccgcctgc tgctgtacac cggctacttc
     2461 gccgtgaccg gcttccagct gctgatcctg aagaactacc tcggcgtgaa ggacccgttc
     2521 gcggtcatcc cgctcctggg catcctcagc ctcgccggcg tgctcatcgc cacggtcatc
     2581 tcgggcccgc tctccgacaa ggtcggccgc cgcaagccct tcgtcttcgg atcctcgctg
     2641 ctgatggccg tcgccctcgt cgtgccctgg atcactccct cgtacacctc gtggatgatc
     2701 atgaccgtga tcagcggatt cgggttcgga atgttccagg cggtggacac cgcgctgatg
     2761 agccaggtgc tcccgtcggc gaagaacttc gcgaaggatc tcggggtcgt gaacatcgcc
     2821 gccacgctgc cccagacgtt cgcccccggt gtcgccggtg cgatcgtgct ggccttcggc
     2881 tacggcgcgc tcttcccgat cgcgatcgtg ctcagcgtgc tcggcgcgat cagcgtctgg
     2941 ttcatcaagt cggtcaagta gtcaaggaga gaacacatga cggacaccga ggtgcgcttc
     3001 ccgtaccagg acgccgcgct cgacatcgag cagcgcgtcg aggacctgct cagccggatg
     3061 gatgccgccg acaaggccgg catgctcttc cacaccatgg tcggcatcgg cgacccgtcc
     3121 gagggcaacc ccacgttcgg gctgccctcg gcgcactcga tgatccgcaa gcgccggatg
     3181 aatcacttca acctgctcgg ctcggcgccc agcggccgcg agttcgccgc gtggagcaac
     3241 gcgctgcagc gcttcgcccg cgaggacggc ctcggcatcc cgatcacgtt ctcgaccgat
     3301 ccgcggcacg cgttcagcga caaccccctg acctcgatgt tcgccggtcc cttctcgcag
     3361 tggcccgaga cgctcggctt cgccgccctc gcctcgccgg agcggctcga gcgcttcagc
     3421 gacatcgcac ggcgggagta cctcgcggtg gggctgcgcg tcgcactgca tccgcagatc
     3481 gacctcgcca ccgagccgcg ctggtcgcgg atcaacggca cgttcggcga gagcgccgac
     3541 ctcaccagcg agctcgtggt cggctacatc cgcgggttca tggggtcgga gctcggcccg
     3601 gagtcggtct cgaccatggt caagcacttc cccggcggcg gtccgcagct cgacggcgag
     3661 gacccgcact tcgagtgggg gaaggaacag gtctaccccg gcggtgagca ggagtaccac
     3721 ctgcgtccgt tcatcgcggc catcggcgcc ggcgccaccc agatgatgcc gtactacggc
     3781 aagccggtcg gcaccgactg ggaggaggtc ggcttcggct tcaacaagga cgtgctgacc
     3841 acgctgctgc gcgaccgcct cggcttcgac ggcatcgtct gcaccgactg gggcccgctc
     3901 accgacgccg acatcctggg gacgccgatg ccggcgcggg cctggggcgt cgagcacctc
     3961 acgcggcccg agcggatggt gaaggcgctg gacgccggca tcgaccagtt cggcggcgag
     4021 ctgtgcaccg acgtgctgct ggagttgctc gcctcgggtg cggtggccga ggaccgtctc
     4081 gacgtctcgg tgcggcgcct gctgcgggag aagttccgcc tggggctgtt cgacgacccg
     4141 ttcgtcgacg aggagcacgc cgaggccgtc gtcggccgtg cggacttcgt gcaggagggc
     4201 ctggacgcgc agagcgattc gctgacgctg ctcacgaaca cggatgcgat cctgccgctg
     4261 acccgcgacg tgaaggtcta cgccgaaggc gtggcatccg acgtcctcgc cgggtatgcg
     4321 accgtcgtcg agacgccggc ggaggccgac gtcgcgatcc tgcgcatcaa ggcgcccttc
     4381 gaggaccgcg accgcggcgg cttctcggcg atgttccact ccgggtcgct ggagttcccc
     4441 gctgaggagc acgcgcgtct gacggcgatc gccgaagccg tgccgacggt gatcgacatc
     4501 tacctcgatc gcccggccgt gctgggcggc ctggaggagt cggctcgcgc cgtgctcgcg
     4561 gactatggcg cctcggacga ggccgtgctg cgggtgctgt tcggcgagcg gggtccgcag
     4621 ggctcgctac cgttcgatct gccgcgttcg gatgccgcag tcgtcgcgag ccgttccgat
     4681 gtcgccttcg acacagagga ccccaccttc cgcttcggcc atggcctgcg gtactgacgc
     4741 cgggagcgcg cgcacgttcg ggaggagttc tcacgttcgg gaggagctct gagccaggat
     4801 gctcctccca tcgcggagtt ctcctcccga acgtcagccc tgctcagccg gcgaccgcgc
     4861 tgctgccggg gatggccgcg atgagctcgc gtgtgtactc gtgcgccggg gctcccagca
     4921 cgtcggcggc gtcgccgctc tcgacgaccc gccccttctg catcacgacg atgcggtcgg
     4981 agatctgccg caccacgccc aggtcgtgcg agatgaacag gtagctcagc ccgaactcgg
     5041 cctgcaggtc gaccagcagc tgcaggatct gctcctgcac ggagacgtcc agcgccgaga
     5101 cgggctcgtc gaggatcatc agctcggggc cgatggcgag cgccctggcg atcgccacgc
     5161 gctgccgctg accgcccgac agctcgccgg gacgccgatc cgccagctcc gacgacaggc
     5221 gcacccgctc gagcagttcg cggaccagca ggcgctgcga agagcggtcc cccacgcgga
     5281 acgaccgcag cggctccgcg acgatccgct cgaccgtcat ccgcgggtcg agcgacccga
     5341 acgggttctg gtagaccacc tgcatccggc ggcgcagctg gcgcaggctc tcaccggtgg
     5401 cacgggtgat gtcctcgccg tcgagcagga tcgtgccggc atccggctgc acgagccgcg
     5461 ccgcgatccg ggcgctcgtg gtcttgcccg aacccgactc gcccacgatc gacagcgtct
     5521 tgccccgcgg gaccgcgaag gacacgtcgt cggcggcgag gaacgatccg ccacgaacgc
     5581 cgtacgtctt gccgaggcct cgcacctcga ggatgctctc ggcgcgcgcc gcgggcgcgg
     5641 aggagcccgc ctgcgccgc
//
LOCUS       DATMSX010000494        21218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_16723,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000494 DATMSX010000000
VERSION     DATMSX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16723"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VN041_15680"
     CDS             complement(<1..659)
                     /locus_tag="VN041_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWU30522.1"
                     /translation="MDLWEMRSLPGGTVTLHDARREQHRTVRLEAFEVGVFAVTEEQF
                     AEVLGIPASRPRHPARDLSWLRAIRFCNAASEWEGLDPAYTFDGEEVTWHVDSDGYRL
                     PTEAEWEFACRAGSTEPQYGPLAEIAWTGADGGSEPHDVGLKLPNLHGLFDTLGNVWE
                     WCWDLLDPERYDDYRVFRGGGFADDSWSVRASVRRGGAPRMHHDDVGMRLARGGFDGV
                     D"
     gene            complement(663..1319)
                     /locus_tag="VN041_15685"
     CDS             complement(663..1319)
                     /locus_tag="VN041_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30523.1"
                     /translation="MRSLAGRRMLRRVPQLLIGLFLYGIGIAFIVRGVLGAAPWDVLS
                     QGIATHLPLSFGTITIIVSGIVLLMWIPLRQKPGVGTVLNALLVGPAADVGFLIIPET
                     DVLWLRVIFFAIGLTVLAAATGLYIGTHFGPGPRDGLMTGLHRVTGRPIWLVRTALEV
                     SVVLVGWLLGGNVGLGTVAFALLVGPMCQFFMRVFEVRLPGEPLTLPGDDLRPDAVAG
                     "
     gene            complement(1316..2071)
                     /locus_tag="VN041_15690"
     CDS             complement(1316..2071)
                     /locus_tag="VN041_15690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU30524.1"
                     /translation="MAEVRFVAIGDSFTEGVGDELPDGTVRGWADIAAQGWADASGHS
                     IRYANLAIRGKLAWPIVEQQLEPALALRPTHLSFNGGGNDMLRPNADIEHLADAFTRV
                     LRRCDEEGVQLIVLSGANPSARLPLGRMVQRRGDELSAAVMRRIEGRPDVVRALNWPD
                     AELSRPQYWSEDRLHMNAHGHHRVAARVLHALGYEPPSEWWALAQHPAVGPSGYAYYR
                     EFVGPWIRRRVTRTSSGDGRTAKYGSWVERTPR"
     gene            complement(2114..3466)
                     /locus_tag="VN041_15695"
     CDS             complement(2114..3466)
                     /locus_tag="VN041_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30525.1"
                     /translation="MPEPSLTSRSSLAARLDALPFTRSHLRVLTGSGVGWALDAMDVG
                     LISFIIAVLAREWSLDSFTTGWIASAGFIGMAIGASVGGLLADRLGRRYVFAVTLLIY
                     GVATGASALVGGVAVLIVLRFFVGLGLGAELPVASTYVSEFAPARIRGRLIVILEAFW
                     AVGWTASALIGYFVIPASESGWRWAFALGAIPAVYALFVRWGLPESPRWLASRGRMGE
                     AERIVSRLETDADLQRTPAIRKEPVRETLAPTTSRRVALLWAPEFRVRTASVWLVWFC
                     VNFAYYGAFIWIPSILFAAGYDLVKSFGFTLLITLAQLPGYAAAAWLIEVWGRRLTLS
                     VFLAGSAVSAVLFGVAGSEGMIIATGMALSFFNLGAWGALYAVTPEIYPTSLRATGAG
                     WAAGIGRIASIIAPLVVPLLLARGGAPLAFVVFSVFFVIAAAAAWGLVDKRGASLDDR
                     "
     gene            complement(3532..5205)
                     /locus_tag="VN041_15700"
     CDS             complement(3532..5205)
                     /locus_tag="VN041_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWU30526.1"
                     /translation="MTAEPRTPSAIDQVAERWVDTLVDLSPTLATYIGRNEANGKLDD
                     LSPEGHERSVAAARATLAELDALEPVDEIDRVTKTDLSSELRLQVELHDAQWHLRDLN
                     VIASAPQDFRQAFDLMPTATAEDWSVVAERLAALPAAIKGYTETLREGIRQGVVPARR
                     QVTEVATQIGRYTADDGFFAAFVAEAAPEEGQLPASLAKELADRSGAARVAYDELRAF
                     LTGELASVAPEQDAVGRELYALNSRRFLGATIDLDETYEWGRDELERMIAEQNSIANE
                     ILPGATVEEAVAHLEADPSRKLHGTEALQRWMQETSDKAIADLGASHFDIPEQIRELE
                     CMIAPTQEGGIYYTGPTDDFSRPGRMWWSVPEGVTEFDTWRELTTVYHEGVPGHHLQI
                     AQATYNRAELNTWRRVLAGTSGHAEGWALYAERLMEQLGYLDDPADRLGMLDGQRMRA
                     LRVVLDIGVHLGKPRLDGDGVWDHDYALDMMRRNVNMSDEFRQFEVNRYLGWPGQAPS
                     YKVGQRIWEQVRDAVRAQEGDAFSFKDFHKRALDMGGVGLDTLRSVFHV"
     gene            complement(5245..7878)
                     /gene="polA"
                     /locus_tag="VN041_15705"
     CDS             complement(5245..7878)
                     /gene="polA"
                     /locus_tag="VN041_15705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWU30527.1"
                     /translation="MTDSAKPTLMVVDGHSLAYRAFFALPVENFTTRDNQHTNAIYGF
                     LSMLVNLIKAEKPTHMAIAFDTSRHSFRTDEYPEYKATRSETPNEFKGQIPLLQDCLA
                     AMSIPVLTKEGIEADDILATLARQGSEKGYDVLVVSGDRDSIQLVNDEVTLLYPSVQG
                     VSQLKRYDPIAVQERYGVRPEQYPDIAALVGETSDNLPGVPKVGEKTAVKWLTQFGTL
                     DELLERSGEIKGVVGGNLREHIEDVRRNRRLNRLLNDVELPVGPDELAVAPIDAQAVR
                     DIFARLEFRTLLPRVFEAVGAADEPVHDEPAVKVPDPTEAAASAAAEWLSAQDGEIGV
                     RLVLNGSRITRFGFASATELHEGDWNDDAAAALRSWLESDAPKVLDDAKPQVKALLRS
                     GIRIGGIAYDTLLAGWLMRPSFPDKTLAHLVGRYLDEKLPEADPTQLVPETDGATPSQ
                     EAWFTLRTAAAIREAMPESVTTVLTEIELPTLRTLADMEVAGVAVSHDVLSTFSGELL
                     TRTDGLAADAFTLVGREFNLGSPKQLQEVLFDDLQLPKTRKTKTGYSTDAAVLADLQE
                     SNPHPFLDLLLQHREATKLRQIIESLDTAIGDDHRIHTTYVQTGSQTGRLSSTDPNLQ
                     NIPVRTEESRRIRSAFEVGEGYESLLTADYSQIEMRIMAHLSGDAGLIEAFNSGEDLH
                     RFVGARVFRVEPEEVTSAMRTKVKAMSYGLVYGLSAFGLSKQLRIEQSEAKQLMMEYF
                     ARFGAVRDYLRASVLKAKEDGYTETIFGRRRPFPDLSSPNRVLRENAERAALNAPIQG
                     SAADIMKIALFRIHADLRQAELRSRALLQIHDELVVEVAPGEWDAVEAIVRDRMGGAA
                     ELSVPLDVQVGRGRDWNEAAH"
     gene            7942..8364
                     /locus_tag="VN041_15710"
     CDS             7942..8364
                     /locus_tag="VN041_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HWU30528.1"
                     /translation="MDEQARMTGMEWATQRGMGALAQKMGIEFTEFSVERSVGTMPVE
                     GNTQPVGLLHGGAYVVLGESLGSMAANLHAGPGRLAVGVDINATHTRSATSGVVTGVC
                     TPIHLGRSMTVHEIVVSDDQGRRCSTIRITNMIKDMPA"
     gene            complement(8426..9043)
                     /locus_tag="VN041_15715"
     CDS             complement(8426..9043)
                     /locus_tag="VN041_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWU30529.1"
                     /translation="MTDVEQPEQPTASAPRRVVVAEDESLIRLDIVEILRDNGFDVVG
                     EAGDGETAVALATELRPDLVVMDVKMPQLDGISAAEKLHKNNIAPVVLLTAFSQKELV
                     ERASEAGALAYVVKPFTPNDLLPAIEIALARHEQIITLEAEVADMVERFETRKLVDRA
                     KGLLNEKMGLSEPEAFRWIQKASMDRRLTMQDVAKAIIEQLAPKK"
     gene            complement(9070..9152)
                     /locus_tag="VN041_15720"
     tRNA            complement(9070..9152)
                     /locus_tag="VN041_15720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9117..9119),aa:Leu,seq:caa)
     gene            complement(9215..10663)
                     /gene="pyk"
                     /locus_tag="VN041_15725"
     CDS             complement(9215..10663)
                     /gene="pyk"
                     /locus_tag="VN041_15725"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWU30530.1"
                     /translation="MRRAKIVATLGPATSTYETVRALIEAGVDVARLNLSHGDYSVHE
                     ANYANVRRAADDAGRAVAVLVDLQGPKIRLGRFEDGPYELAVGDIFKITTEDIIGNRE
                     ICGTTFKGLPQDVKAGDFLLIDDGKVRVEVVDTDGTVVTTRVVVAGAVSNNKGINLPG
                     VAVNVPALSEKDEDDLRWGLRTGADLIALSFVRNASDVERVHEIMAEEGVRVPVIAKI
                     EKPQAVDALEEIVDAFDGIMVARGDLGVELPLEAVPIVQKRAVELARRNAKPVIVATQ
                     MLESMINSPVPTRAETSDVANAVLDGADAVMLSGETSVGEYPIVVVETMARIIESTEE
                     HGLERISPLTNKPRTQGGAVTLAALEVAEFVDAKFLCVFTQSGDSARRLSRLRSRIPM
                     LAYTPEPGIRRRMALTWGIRSTLVEMVQHTDAMYHQVDEHLLGSGLAKEGDKVVVISG
                     SPPGIIGSTNDIRIHKVGDAVGGAAPIYKSGV"
     gene            complement(10720..12186)
                     /locus_tag="VN041_15730"
     CDS             complement(10720..12186)
                     /locus_tag="VN041_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWU30531.1"
                     /translation="MADPKGFLKTTQRELPARRPVAVRIMDWKEVYEDTDRTVLRRQA
                     GRCMDCGVPFCHSGCPLGNIIPEWNDLTWRDERQAASDRLHATNNFPEFTGRLCPAPC
                     ESACVLGINQPAVTIKHIEQSIADDAIESGWVQPRPPQRLTGKTVAVVGSGPAGLAAA
                     QQLTRAGHTVVVYERDDRIGGLLRYGIPDFKMEKRHVDARLRQMEEEGTRFRPGVEIG
                     VQLSWSDLRARYDAVLIATGSTVPRDLPIPGRDLPGVHFAMDYLVQSNHAVAGDEVPG
                     QISAAGKHVVVIGGGDTGADCIGTAHRQGARSVTNLAIGRRPGTERSEHQPWPTQPTL
                     FEVQSAHEEGGERTYLASTVEFLSDDDGALRALRVAETAFVDGKRVPQSGTEREIPAE
                     LVLIAMGFTGPEADVLGERDVPALTGRGVYQRDARFASSEPGVYVAGDAGRGQSLIVW
                     AIAEGRAAAAAIDRDLMGATTLPAPVAATDVALSVQPA"
     gene            complement(12179..16684)
                     /gene="gltB"
                     /locus_tag="VN041_15735"
     CDS             complement(12179..16684)
                     /gene="gltB"
                     /locus_tag="VN041_15735"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWU30532.1"
                     /translation="MYNPAHEKDACGLAMVATLRGEPGHDIIALALEALRNLEHRGAI
                     GSDAGTGDGAGILTQMPDAFLRAVTDFDLPPVGEYAAGLAFLPLESSARREQKAGIER
                     IATEEGLVVLGWRVVPTANEHLGKLADEARPAFEQLFITAPGAVSGIALDRLAYRLRK
                     RAGHEVGAYFVSLSARTLGYKGMVTTLQLEPFYPDLQDERFASELAVVHSRYSTNTFP
                     SWPLAQPLRMLAHNGEINTIGGNRNWMRARQSQLESELLGDMAPLLPICTEGASDSAS
                     FDEVLELLTLTGRSLPHAVMMMVPAAWEKQTDLDPDLRAFYEYHANQMEPWDGPAALI
                     FTDGVQVGATLDRNGLRPGRWTETTDGLVVIGSETGVLEFAPERIKRRGRLQPGRMFL
                     VDTAQRRIIEDDEVKRDLAALHPWADWLAEGAVRLADLPEREHIVHPAASITRRQRTF
                     GYTEEEVRILLTPMGQNGTEPLGAMGSDAPIAALSERPRLLFDYFVQQFAQVTNPPLD
                     AIREEVVTSLRLGLGPERNLLDWGPDHARTVSLDFPVIDNDELAKIRHIDRALPGRSS
                     ATIRGLYHFDAGPHTLADRLAAMCAEADAAIERGAELLILSDRDSNKDLVPIPSLLMV
                     AAVHHHLIRRENRMKVGLVVEAGDVREVHHVATLIGYGASAVNPYLAMETVEHLVRTG
                     YITGISPEEAVHNVIHALGKGVLKIMSKMGISTVGSYAGAQVFEAIGLSQELVDAYFT
                     GTETKLGGIGIEDVFAENQARHDYAYPEDEAARAHERLWIGGEYQWRRDGSPHLFNPD
                     TVFRLQHSTRTRRYDIFREYTKLVDDQAHELKTLRGLFELRTGERPPVPLDEVEPVSA
                     IVKRFSTGAMSYGSISQEAHETLAIAMNRLGAKSNTGEGGEDPERLVDPERRSAIKQV
                     ASGRFGVTSQYLTEATDLQIKLAQGAKPGEGGQLPPQKVYPWVARTRHATPGVGLISP
                     PPHHDIYSIEDLKQLIFDLKRANPKARIHTKLVSQSGIGAVAAGVAKALSDVILVAGH
                     DGGTGASPLNSLKHAGTPWELGLAETQQTLMLNGMRDRVVVQVDGQLKTGRDVIIGAL
                     LGAEEFGFATAPLVVSGCIMMRVCHLDTCPVGVATQNPLLRSRFTGKPEFVVNFMEFI
                     AEEVREHLAALGFRSLDEIIGHTELLRTDAAVHHWKTEGLDLSPILTGPVFPSGEPRK
                     SGRTQDHELHKHFDTALIALSGDALEQGDPVRIDLPIRNTERAVGTMLGHHVTERYGA
                     AGLPPETIDVTLRGTAGQSLGAFMPAGIMLRLEGDANDYVGKGLSGGDIVIRPPRDAA
                     FEPHRNVIAGNVIGYGATSGTMFISGVVGERFLVRNSGATAVVEGVGDHALEYMTGGL
                     AVILGTTGRNLGAGMSGGVAYVHALRETQVNAEALESGELQLVPLDDADAATLHHLLT
                     EHVERTGSPLATELLADADALRTAFVKVLPRDYAAVQEIRREAQAAGDDPDGENIWTR
                     ILEVTRG"
     gene            complement(16846..17844)
                     /gene="lgt"
                     /locus_tag="VN041_15740"
     CDS             complement(16846..17844)
                     /gene="lgt"
                     /locus_tag="VN041_15740"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWU30533.1"
                     /translation="MSLALHSLISGVPASIPSPPVSTFPIFGITIHYYALCIIAGIIA
                     ATLITNHRLTKRGAEKWVVIDIAIIAVPLAIIGARIFHVLTHFDFYFGPGKNTWNPFE
                     PGSVWAIWEGGIAIFGALMAGAVGAWLGCRWTGIRFWTFADALAPGLLLAQAMGRFGN
                     WFNHELFGLPTDLPWGLQIESTNPAFPAGLPEGTLFHPTFLYEVIWNTLGVLVLLWLG
                     RRLRLQWGRLFACYLIWYSAGRIVWESIRIDPSQVYLGLRSNVWAAIIGVLVGLVILI
                     VQTRRHTGLEASPYQPGREPADADVDSDHDSHDYVDLSEPPVDGIDAEAPATSTAS"
     gene            complement(17855..18658)
                     /gene="trpA"
                     /locus_tag="VN041_15745"
     CDS             complement(17855..18658)
                     /gene="trpA"
                     /locus_tag="VN041_15745"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWU30534.1"
                     /translation="MSRVEAAIRNAHDAGRGAFIGYLPIGFPDLETGVEAALTLARNG
                     VDVIELGPPYSDPVMDGEVIQQATQQALANGYRTRDLFTVVKEITSRTDVPVVVMTYW
                     NPVLQYGVDRYADDLLAAGGAGLITPDITPEAAGEWIAASTRTGLDRIFLAAPTSTDE
                     RLDLVISSSTGFVYTVSTMGITGERAALDRAARTLVDRMRARGAASACVGIGISNPEQ
                     IAGVVEYADGAIVGTALVKALRDGGLEGLAETTRSLAGGTPSARFATGN"
     gene            complement(18655..19863)
                     /gene="trpB"
                     /locus_tag="VN041_15750"
     CDS             complement(18655..19863)
                     /gene="trpB"
                     /locus_tag="VN041_15750"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWU30535.1"
                     /translation="MALRDQHGPFFGEYGGRFMPESLIAAIDELTVAYEAAIVDPAFR
                     TELATLLHSYAGRPSPITEVPRFAEHAGGARVFLKREDLNHTGSHKINNVLGQALLTK
                     RLGKTRVIAETGAGQHGVATATAAALFGLDCTIYMGEVDTERQALNVARMRLLGAEVV
                     PVKTGSRTLKDAINDAYRDWVATVETTNYIFGTAAGPHPFPAMVRDFQKIIGEEAREQ
                     LLAEAGRLPDAVVACVGGGSNAIGMFDAFLDDEGVKLYGVEAAGDGVDTPKHAASIER
                     GRPGVLHGAKTYVLQDEDGQTVESHSISAGLDYPGVGPEHAWLSDIGRAEYIPATDDE
                     AMQALRLLSRTEGIIPAIESAHALAGALRIGRELGPDAVIAVCLSGRGDKDMDTAARY
                     FDLYDEEDAS"
     gene            complement(19866..20669)
                     /gene="trpC"
                     /locus_tag="VN041_15755"
     CDS             complement(19866..20669)
                     /gene="trpC"
                     /locus_tag="VN041_15755"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWU30536.1"
                     /translation="MVLADLTAGAVQDAERRAATRPLADVEREALARPAARDALAALT
                     PADRVKIIAEVKRASPSRGALAEIPDPALQASLYEQGGASAISVLTEERRFGGSLADL
                     EAVTARVDLPVLRKDFIATPYQVLEARAAGADLALLIVAGLDVKTLRELYALIRELGM
                     TPLIETHSAEELEVAIDLGAELIGVNARNLKTLELDRDLFASLSERIPDTAIRVAESA
                     VLAPEDVRRYREAGADVVLIGEALVTGEPVATLNAFLAAGDLPVRGGEE"
     gene            complement(20669..20908)
                     /locus_tag="VN041_15760"
     CDS             complement(20669..20908)
                     /locus_tag="VN041_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HWU30537.1"
                     /translation="MTNPIADPGHGHSPAAWTAVVIMLVGFTAGTVFFCFPDGSGVVG
                     VWISAVVVVIGAIVGWVMAKAGWGVKGPKYTPKAH"
     gene            complement(20943..>21218)
                     /locus_tag="VN041_15765"
     CDS             complement(20943..>21218)
                     /locus_tag="VN041_15765"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HWU30538.1"
                     /translation="LTKATGLSGDKALGAMVTDLEPSGWGVAALCAWVVVALASLFVL
                     ATAHRWKAGTRRYDQLSDGRHADAGPLDAVDSWDDLSHGTDPTGPSR"
BASE COUNT         3206 a         7215 c         7505 g         3292 t
ORIGIN      
        1 ctgtccaccc cgtcgaatcc gccacgggcg agccgcatgc cgacgtcgtc gtggtgcatg
       61 cggggcgcgc cgcctcgccg gaccgacgcc cgcacgctcc agctgtcatc cgcgaagccg
      121 ccaccgcgga agacgcggta gtcgtcgtag cgttcggggt cgagcaggtc ccagcaccac
      181 tcccagacgt tcccgagcgt gtcgaacagc ccgtggaggt tgggcagctt cagcccgacg
      241 tcgtgcggct cggagcctcc gtcggccccg gtccaggcga tctccgcgag cgggccgtac
      301 tgcggctctg tcgagccggc ccggcacgcg aactcccatt cggcctccgt cgggagccga
      361 tacccgtcgc tgtcgacgtg ccaggtgacc tcctcgccgt cgaacgtgta ggccgggtcg
      421 agcccctccc actccgacgc ggcattgcag aatcggatcg cgcgcagcca gctgagatcc
      481 cgcgccgggt gccgcggacg ggaggccggg atgccgagca cctccgcgaa ctgctcctcg
      541 gtcaccgcga acacgccgac ctcgaacgcc tcgagcctga ccgtgcgatg ctgctcccgc
      601 cgcgcgtcgt gcagggtgac cgttccgccg ggaagcgacc gcatctccca cagatccatg
      661 tctcagcccg cgaccgcgtc gggacggaga tcgtcacccg gcagagtcag tggttcgcca
      721 ggcagccgca cctcgaagac gcgcatgaag aactgacaca tcgggccgac cagcagggcg
      781 aacgccactg tgccgagccc gacgttcccg cccagcagcc agccgacaag caccacagac
      841 acctccagtg ccgtgcggac caaccagatc gggcgaccgg tgacgcggtg caggccggtc
      901 atcagcccat cccgcggacc gggaccgaaa tgcgtcccga tgtacaggcc cgtggccgcg
      961 gcgagcacgg tgagtccgat cgcgaagaag atcacgcgaa gccacagcac gtcggtctcg
     1021 gggatgatca ggaagccgac atctgcggcg ggtcccacca gcagtgcgtt cagcaccgtg
     1081 ccgacgcccg gtttctgacg caacgggatc cacatcagca gcacgatgcc gctgacgatg
     1141 atggtgatcg tcccgaagga aagcgggagg tgcgtcgcga tgccctggga caggacgtcc
     1201 cagggagcgg cgccgagcac gccccgcacg atgaaggcga tgccgatccc gtacaggaaa
     1261 aggccgatga gcaactgcgg cacccggcgc agcatccggc gtccggcgag tgacctcatc
     1321 gaggtgtccg ctcgacccag gatccgtact tcgcggtgcg cccgtcaccg gacgaggtgc
     1381 gggtgacgcg tcgtctgatc cacgggccga cgaactcgcg gtagtacgca tagccgctcg
     1441 gccccacggc cggatgctgt gccagagccc accactcgct cggcggctcg tagccgagtg
     1501 cgtgcagcac gcgcgccgcg acgcggtgat gaccgtgcgc gttcatatgc agtcggtcct
     1561 cggaccagta ctgcggacgg gacagctcgg cgtcgggcca gttcagcgcg cggacgacat
     1621 cgggtcggcc ctcgatgcgg cgcatcacgg cagccgacag ctcgtcccca cgtcgctgca
     1681 ccatccggcc cagcgggaga cgcgcactcg ggttcgcgcc cgagagcacg atgagctgca
     1741 cgccctcctc atcgcacctg cgcagcacgc gggtgaaggc gtcggccagg tgctcgatgt
     1801 cggcgttcgg gcgcagcatg tcgttgccgc cgccgttgaa ggagagatgt gtcggccgca
     1861 gagcgagtgc gggctcgagc tgctgctcga cgatcggcca ggccagtttg ccccggatgg
     1921 cgaggttcgc atagcggatg ctgtgccctg acgcatcggc ccagccctgc gccgcgatgt
     1981 cagcccagcc ccgcacggtg ccgtccggca gctcgtcgcc gacgccctcc gtgaaggagt
     2041 cgccgatcgc cacgaatcgc acttccgcca tcgttccagc ctatggccgc gccggaccgc
     2101 tcggcgccaa gggtcagcgg tcgtcgaggc tcgcaccgcg cttgtcgacg agcccccagg
     2161 ccgccgccgc cgcgatcacg aagaacaccg agaacaccac gaaggccaac ggcgcaccgc
     2221 cccgtgcgag cagcagcggc accaccagcg gcgcgatgat ggacgcgatg cgtccgatgc
     2281 cggcggccca gcccgctccg gtcgcgcgca gcgaggtcgg gtagatctcg ggcgtcacgg
     2341 cgtacagcgc accccaggct ccgagattga agaacgagag cgccataccg gtcgcgatga
     2401 tcatgccctc gctcccggcg acgccgaaga gcacggccga caccgcagag cccgccagga
     2461 acaccgacag cgtcagacgc ctgccccaca cctcgatcaa ccacgccgcg gcggcgtacc
     2521 ccggcagttg cgcaagcgtg atcagcagag tgaaaccgaa cgacttcacc aggtcgtagc
     2581 cggcggcgaa gaggatgctg gggatccaga tgaacgcacc gtagtaggcg aagttcacgc
     2641 agaaccacac gagccagaca ctggcggtgc ggacacggaa ctcgggcgcc cacagcagcg
     2701 ccactcgccg ggatgtggtg ggggccaggg tctcgcgcac cggctccttg cggatcgccg
     2761 gggtgcgctg cagatcggca tccgtctcca gacgggagac gatccgctcg gcctcgccca
     2821 tccggccgcg cgacgccagc cagcgcggtg actccggcag accccaccgc acgaagaggg
     2881 cgtagaccgc cgggatcgcg ccgagcgcga acgcccaccg ccagccgctc tcggaggcgg
     2941 ggatcacgaa gtagccgatc agggcggatg ccgtccagcc gaccgcccag aacgcctcca
     3001 ggatcacgat gagtcgaccg cggatgcgcg cgggcgcgaa ctcgctgacg tacgtggatg
     3061 ccaccggaag ctcggctccg agccccaggc ccacgaagaa ccggagcacg atgagcaccg
     3121 ccacgccgcc gaccagcgca ctcgccccgg tcgccacgcc gtagatcagc agtgtgacgg
     3181 cgaagacgta ccggcgcccc agccggtcgg cgagcagccc gccgacgctg gctccgatcg
     3241 ccattccgat gaacccggcc gatgcgatcc acccggtcgt gaacgaatcg agcgaccact
     3301 cccgggcgag caccgcgatg atgaacgaga tgaggccgac gtccatggca tccagggccc
     3361 agccgacgcc ggagcccgtc agcacgcgca gatgcgaccg cgtgaacggc agagcgtcga
     3421 gtcgtgcagc cagggacgac cggctcgtca gcgagggttc gggcatgatc tcatcgtaga
     3481 ggtgaggtca gcatgaccac acctctacga cgagaagctc gcgcgcgagt ctcagacgtg
     3541 gaacaccgac cgcagcgtgt ccaggccgac gccacccatg tcgagggcgc gcttgtggaa
     3601 gtccttgaac gagaacgcgt cgccctcctg ggcgcggacg gcgtcgcgga cctgctccca
     3661 gatgcgctgg ccgaccttgt acgacggcgc ctggcccggc catccgaggt agcggttcac
     3721 ctcgaactgg cggaactcgt cggacatgtt cacgttgcgc cgcatcatgt ccagcgcgta
     3781 gtcgtggtcc cagacaccgt cgccgtcgag gcgcggcttg ccgaggtgaa cgccgatgtc
     3841 cagcacgacg cgcagggcgc gcatgcgctg accgtcgagc atgccgagcc ggtccgcagg
     3901 gtcgtcgagg tagccgagct gctccatcag acgctcggcg tacagggccc acccctcggc
     3961 gtggccggag gtgccggcga gcacacgccg ccaggtgttc agttcggcac ggttgtaggt
     4021 cgcctgcgcg atctgaaggt ggtggccggg gaccccctcg tggtacaccg tggtgagctc
     4081 gcgccaggtg tcgaactcgg tgacgccctc gggaacggac caccacatcc ggccgggacg
     4141 cgagaagtcg tcggtcgggc cggtgtagta gatcccgccc tcctgggtag gggcgatcat
     4201 gcactccagc tcgcggatct gctccgggat gtcgaagtgg ctggcgccga ggtcggcgat
     4261 ggccttgtcg ctggtctcct gcatccagcg ctgcagtgcc tcggtcccgt gcagcttgcg
     4321 ggacggatcg gcctcgaggt gcgcgaccgc ctcctcgacc gtggctccgg ggaggatctc
     4381 gttcgcgatc gagttctgct cggcgatcat gcgctcgagc tcgtcgcggc cccactcgta
     4441 ggtctcgtcg aggtcgatcg tggcgccgag gaagcggcgg gagttcagcg cgtagagctc
     4501 gcggccgaca gcatcctgct ccggtgccac cgaagccagt tcgccggtga ggaaggcgcg
     4561 cagctcgtcg taggcgacgc gggcggcgcc ggagcggtcg gcgagctcct ttgcgaggga
     4621 cgccggcagc tgcccctcct cgggagcggc ctcggcgacg aacgccgcga agaagccgtc
     4681 atccgcggta tagcggccga tctgggtggc cacttcggtc acctgccggc gggcggggac
     4741 gacgccctgg cggatgccct cgcgcagcgt ctcggtgtag cccttgatgg ccgcgggcag
     4801 tgcggccagg cgctccgcga cgaccgacca gtcctcggca gtcgccgtgg gcatcagatc
     4861 gaaggcctgt cggaaatcct gaggcgcgga tgcgatcacg ttcaggtcgc gcaggtgcca
     4921 ctgcgcgtcg tgcagctcca cctgcaggcg cagctcggag gacaggtcgg tcttggtgac
     4981 ccgatcgatc tcgtcgacgg gctccagtgc gtcgagctcg gcgagtgtcg cgcgtgcggc
     5041 ggcgaccgag cgctcgtggc cctcggggct gaggtcgtcg agctttccgt tcgcctcgtt
     5101 acggccgatg taggtcgcca gcgtcggcga cagatccaca agggtgtcca cccagcgctc
     5161 ggcgacctgg tcgatcgcgg agggggtgcg gggttcagca gtcatggatt cgagcctagc
     5221 cccgcacggc cgtgtgtccg gggatcagtg cgctgcttcg ttccagtcgc gtccacggcc
     5281 gacctgcacg tccagcggca ccgacagctc ggcggccccg cccatgcggt cgcgcacgat
     5341 cgcctccacg gcatcccact cccccggtgc gacctccacg acgagttcgt cgtggatctg
     5401 cagcagggcg cgggaacgaa gctccgcctg gcgcaggtcg gcgtggatgc ggaacagggc
     5461 gatcttcatg atgtcggccg cgctgccctg gatcggagcg ttgagcgcgg cgcgctcggc
     5521 gttctcgcgg agcacccggt tggggctgct caaatccggg aacggacgcc ggcggccgaa
     5581 gatcgtctcg gtgtagccgt cttccttcgc cttcagcacc gaggcccgca ggtagtcgcg
     5641 caccgcgccg aatcgcgcga agtactccat catcagctgc ttggcctcgg actgctcgat
     5701 gcgcagctgc ttggacaggc cgaaggccga gagcccgtag acgagaccgt acgacatcgc
     5761 cttgaccttg gtgcgcatgg ccgaggtgac ctcctcgggc tccacacgga agaccctcgc
     5821 gccgacgaac ctgtgcaggt cctcaccgga gttgaacgcc tcgatcagcc ctgcgtcgcc
     5881 ggacagatgc gccatgatgc gcatctcgat ctgcgagtag tcggcggtca gcagggactc
     5941 gtagccctcc ccgacctcga aggcgctgcg gatgcgacgg gactcctcgg tgcgcaccgg
     6001 gatgttctgc aggttggggt cggtgctgga cagacgtccg gtctggctcc cggtctgcac
     6061 gtaggtggtg tggatgcgat ggtcgtcgcc gatggcggtg tccagcgact cgatgatctg
     6121 gcggagcttg gtggcctcgc ggtgctgcag cagcagatcc aggaacgggt gcggattgct
     6181 ctcctgcaga tcggcgagca ccgccgcatc cgtggagtag ccggtcttgg tcttgcgggt
     6241 cttgggcagc tggaggtcgt cgaacagcac ctcctgcagc tgcttgggcg agccgaggtt
     6301 gaactcccga ccgaccagcg tgaacgcgtc cgcggcgagc ccgtcggttc gggtgagcag
     6361 ctcgcccgag aacgtggaca gcacgtcgtg ggagacggcg acgcccgcga cctccatgtc
     6421 ggcgagcgtg cgcagggtag gcaactcgat ctcggtcagc accgtcgtca ccgactccgg
     6481 catcgcctca cggatcgcgg cggcagtgcg cagcgtgaac cacgcctcct gcgagggcgt
     6541 cgcgccgtcg gtctcgggca cgagctgcgt gggatcggcc tcgggcagct tctcgtcgag
     6601 gtagcggccg acgaggtgcg cgagggtctt gtccgggaag ctcgggcgca tcagccagcc
     6661 cgcgagcagc gtgtcgtagg cgatgccgcc gatgcggatg ccggaccgca gcagggcctt
     6721 cacctgcggc ttcgcgtcgt cgagcacctt gggcgcgtcg gactccagcc aactgcgcag
     6781 cgcggccgcg gcgtcgtcgt tccagtcgcc ctcgtgcagt tccgtcgcac tggcgaagcc
     6841 gaagcgcgtg atcctcgagc cgttgagcac gaggcgcacg ccgatctcgc cgtcctgagc
     6901 ggagagccac tcggctgcgg ccgacgcggc cgcctcggtg gggtcgggca ccttcaccgc
     6961 gggctcatcg tgcacgggct cgtcggcggc cccgaccgcc tcgaagacgc gcggcagcag
     7021 ggtgcggaac tcgaggcgtg cgaagatgtc acgcaccgcc tgggcatcga tgggggccac
     7081 agccagctcg tcggggccga caggcagctc gacgtcgttc agcaggcggt tcagccgccg
     7141 gttgcggcgc acgtcctcga tgtgctcacg gaggttgccg ccgacgacgc ccttgatctc
     7201 gccggagcgc tcgagcagct cgtcgagggt cccgaactgc gtcagccact tgacggccgt
     7261 cttctccccc accttcggca cgccggggag gttgtcgctg gtctcgccga cgagcgccgc
     7321 gatgtcggga tactgctccg gccgcacacc gtagcgctcc tgcacggcga tcgggtcgta
     7381 gcgcttgagc tgtgacaccc cctggacgga ggggtacagc agcgtcacct cgtcgttcac
     7441 gagctggatc gagtcgcggt cgccggagac gaccagcacg tcgtacccct tctcggagcc
     7501 ctgacgggcg agggtcgcga ggatgtcgtc ggcctcgatg ccctccttgg tgagcaccgg
     7561 gatggacatc gcggccaggc agtcctggag cagcgggatc tggcccttga actcgttcgg
     7621 agtctcggag cgcgtcgcct tgtactccgg gtactcgtca gtgcggaacg agtgccgtga
     7681 ggtgtcgaag gcgatcgcca tgtgggtggg cttctcagcc ttgatgaggt tcaccagcat
     7741 cgacaggaag ccgtagatgg cgttcgtgtg ctgattgtcc ctggtcgtga agttctcgac
     7801 cggaagggcg aagaaggccc ggtacgcgag cgagtggccg tcgacgacca tgagggtagg
     7861 ctttgcggag tccgtcaccc tgcaagccta acgagagcga cggacactcg ccgaggagga
     7921 cgtgtgacgg atcaggctca gatggacgag caggctcgga tgactggcat ggagtgggcc
     7981 acgcagcgcg gcatgggcgc gctggcgcag aagatgggga tcgagttcac cgagttctcc
     8041 gtcgagcgca gcgtcggcac gatgcccgtg gagggcaaca cgcagccggt cggcctgctg
     8101 catggcggcg cctacgtcgt gctgggcgag tcgctcggtt ccatggctgc caatctgcac
     8161 gccgggccgg gccgtctcgc cgtcggcgtc gacatcaatg cgacccacac gcgctccgcg
     8221 acctccggcg tcgtcaccgg tgtctgcacg ccgatccacc tcggtcgcag catgaccgtg
     8281 cacgagatcg tggtctccga cgatcagggc cgacgctgct ccacgatccg catcaccaac
     8341 atgatcaagg acatgcccgc ctgacgcgga tgcgagtgtg gccccgccat caggcggggc
     8401 cacactcgat gtcagtggat cgtgctcact tcttgggagc gagctgctcg atgatcgcct
     8461 tggcgacgtc ctgcatcgtg agacggcggt ccatcgatgc cttctggatc cagcggaacg
     8521 cctccggctc gctcaggccc atcttctcgt tcaacagtcc cttggcacgg tcgacgagct
     8581 tgcgggtctc gaagcgctcg accatgtcgg cgacctctgc ctcgagcgtg atgatctgct
     8641 cgtgccgggc cagcgcgatc tcgatcgcag gcagcaggtc gttcggcgtg aagggcttga
     8701 ccacgtacgc cagtgcgccg gcctcgctgg cgcgctcgac gagctccttc tggctgaatg
     8761 cggtcagcag cacgacgggc gcgatgttgt tcttgtggag cttctcggcc gcgctgatgc
     8821 cgtcgagctg aggcatcttc acgtccatca cgaccaggtc ggggcgcagc tcggtggcaa
     8881 gggccactgc ggtctcgccg tcgcccgcct cgccgacgac gtcgaagccg ttgtcacgga
     8941 ggatctcaac gatgtcgagg cggatcagcg actcgtcctc ggcgacgacc acgcgtcgcg
     9001 gggcggatgc cgtgggctgc tcaggctgtt ctacgtcagt cacggtaaga atcctatctg
     9061 ttcaccttgt gccggcggtg ggactcgaac ccacacgtcc tctcggacaa agcattttga
     9121 gtgctccgcg tctgccattc cgccacgccg gcccggatcg actttagcgc acgacgaagg
     9181 ggcggacccg caggcccgcc ccttcgaaag cgtctcagac gccgctcttg tagatcggcg
     9241 ccgctccacc cacggcatcc cccaccttgt ggatgcggat gtcgttcgtg gagccgatga
     9301 tgccgggagg ggaaccggag atcacgacga ccttgtcccc ttccttcgcc aggccagagc
     9361 cgagcaggtg ctcgtccacc tggtggtaca tcgcgtcggt gtgctgcacc atctcgacga
     9421 gggtcgagcg gatgccccag gtcagcgcca tgcggcggcg gatgccgggc tcgggcgtgt
     9481 acgcgagcat cgggatgcgc gagcgcagcc gcgagaggcg acgtgcggag tcgccggact
     9541 gcgtgaacac gcacaggaac ttcgcgtcga cgaactccgc gacctccagc gccgcgagcg
     9601 tcaccgcgcc gccctgagtg cgcggcttgt tggtgagcgg ggagatccgc tccaggccgt
     9661 gctcctcggt cgactcgatg atgcgggcca tggtctccac gaccacgatc gggtactccc
     9721 ccacgctcgt ctcgccggac agcatcacgg cgtccgcgcc gtcgagcacg gcgttggcga
     9781 cgtccgaggt ctcggcgcgg gtcgggacgg ggctgttgat catagactcc agcatctgcg
     9841 tggcgacgat caccggcttc gcgttgcgac gggcgagttc caccgcgcgc ttctgcacga
     9901 tcggcaccgc ctcgaggggc agctcgacgc ccaggtcgcc acgggcgacc atgatgccgt
     9961 cgaacgcgtc gacgatctcc tcgagggcgt ccacggcttg cggcttctcg atcttggcga
    10021 tcaccgggac gcgcacgcct tcctcggcca tgatctcgtg cacgcgctcc acgtcgctcg
    10081 cgttgcggac gaacgacagc gcgatcaggt cggcgccggt gcgcagaccc cagcgcaggt
    10141 cgtcctcgtc cttctcgctc agagcgggga cgttcaccgc gacgccgggc aggttgatgc
    10201 ccttgttgtt cgacacggcg ccggcgacga ccacgcgggt cgtgacgacg gtgccgtcgg
    10261 tgtccacgac ctcgacgcgc accttgccgt cgtcgatgag caggaagtcg cccgccttga
    10321 cgtcctgcgg gaggcccttg aacgtcgtgc cgcagatctc ccggttgccg atgatgtcct
    10381 cggtggtgat cttgaagatg tctccgacgg cgagctcgta gggtccgtcc tcgaaacggc
    10441 cgaggcggat cttcgggccc tgaaggtcga ccagcacggc gacggcgcgt ccggcgtcgt
    10501 cggcagcccg gcgcacgttg gcgtagttgg cctcgtgaac ggagtagtcg ccgtggctga
    10561 ggttcagccg tgcgacgtcc acaccggcct cgatcagtgc acggaccgtt tcataggtcg
    10621 aggttgcagg cccgagtgtg gccacgatct tcgcgcgtct caacggcatc tccagggtga
    10681 gaaggggtac ggggacttgg cgacgttgcc acgctcagcc tacgcaggct ggacgctgag
    10741 tgcgacatcc gttgcagcga ccggtgcggg cagcgtcgtg gcgcccatca ggtcgcgatc
    10801 gatggcggct gcggcggcac gaccctcggc gatcgcccag acgatcaggg actgaccgcg
    10861 cccggcgtcg ccggcgacgt agacgccggg ctcggaggat gcgaaccgcg cgtcgcgctg
    10921 gtacacgccg cgaccggtga gggcgggcac gtcccgctcg ccgagcacgt cggcctccgg
    10981 tccggtgaag cccatcgcga tgagcacgag ctcggcgggg atctcccgct ccgtcccgct
    11041 ctgcggaacc cgcttgccgt cgacgaacgc ggtctccgcg acgcgcaggg cgcgcagggc
    11101 cccgtcgtcg tcggagagga actcgacggt cgaggcgagg taggtgcgct caccgccctc
    11161 ctcgtgcgcg gactgcacct cgaacagggt gggctgcgtc ggccagggct ggtgctcgct
    11221 gcgctccgtg ccggggcgcc gtccgatggc caggttggtc acgctgcgcg cgccctggcg
    11281 gtgtgcggtc ccgatgcagt cggcgccggt gtcaccgccg ccgatgacca cgacgtgctt
    11341 cccggccgcg ctgatctggc cggggacctc gtcgccggcg acagcgtggt tggactgcac
    11401 caggtagtcc atcgcgaaat gcacgccggg cagatcgcgg ccggggatcg gcagatcacg
    11461 cggcactgtg gagcctgtgg cgatcagcac ggcgtcgtag cgcgcccgca ggtcgctcca
    11521 ggacagctgc accccgatct cgacgcccgg gcggaaacgg gtgccctcct cctccatctg
    11581 acgcagacgc gcgtcgacgt ggcgcttctc catcttgaag tcggggatgc cgtagcgcag
    11641 cagcccgccg atgcggtcgt cacgctcgta cacgaccaca gtgtgcccgg cgcgcgtgag
    11701 ctgctgggcg gccgcgagcc cggcgggacc ggacccgacc accgcgaccg tcttccccgt
    11761 gagccgctgc ggcggacgag gctgcaccca gccgctctcg atggcatcgt cggcgatcga
    11821 ctgctcgatg tgcttgatcg tgacggcggg ctggttgatc cccagcacgc aggcgctctc
    11881 gcacggtgcc gggcacagcc gtccggtgaa ctccgggaag ttgttcgtgg cgtgcagccg
    11941 gtcgctcgcg gcctgccgct cgtcgcgcca ggtcaggtcg ttccactccg ggatgatgtt
    12001 gcccagcggg catccggagt ggcagaacgg cacgccgcag tccatgcagc gccccgcctg
    12061 gcggcgcagc acggtgcggt cggtgtcctc gtagacctct ttccagtcca tgatgcgcac
    12121 cgccaccgga cgtcgcgccg gcagctcgcg ctgagtcgtc ttcaggaagc ccttcgggtc
    12181 agccacgggt cacctccagg atccgggtcc agatattctc gccgtcgggg tcgtccccgg
    12241 cggcctgcgc ctcgcggcgg atctcctgca cggcggcata gtcccgcggc agcaccttga
    12301 cgaaggccgt cctcagcgcg tcggcgtcgg cgagcagctc ggtcgccagc ggcgaacccg
    12361 tgcgctcgac gtgctcggtc agcaggtggt gcagggtcgc ggcgtcggcg tcgtcgagcg
    12421 gcacgagctg gagctcgccg ctctcgagcg cctccgcgtt cacctgcgtc tcgcgcagtg
    12481 catgcacata tgcgacaccg cccgacatgc cggccccgag gttgcgtccc gtggtgccga
    12541 ggatcacggc aagtccgccc gtcatgtact cgagggcgtg gtcgccgaca ccctccacga
    12601 ctgccgtcgc gcccgagttg cgcaccagga accgctcccc cacgacaccg gagatgaaca
    12661 tcgtccccga ggtggcgccg tacccgatca cgttgcccgc gatcacgttc cgatgcggtt
    12721 cgaaggcggc atccctgggc gggcggatca cgatgtcccc accggagagg cccttgccga
    12781 cgtagtcgtt ggcgtcccct tcgagccgga gcatgatgcc cgccggcatg aacgcgccga
    12841 gcgactgccc ggccgtgccc cggagggtca cgtcgatggt ctccggcggg aggccggcgg
    12901 cgccgtagcg ctccgtgacg tggtggccca gcatcgtccc cacagcgcgc tccgtgttgc
    12961 ggatcggcag gtcgatgcgc accgggtcgc cctgctcgag ggcgtccccg gacagggcga
    13021 tcagcgcggt gtcgaagtgc ttgtgcagct cgtggtcctg cgtccggccg ctcttgcgcg
    13081 gctcgccgga agggaacacc ggcccggtga ggatcggcga caggtccagg ccctcggtct
    13141 tccagtggtg gacggcggcg tcggtgcgca gcagctcggt gtgaccgatg atctcgtcga
    13201 gcgatcggaa gccgagcgcc gcgaggtgct cgcgcacctc ttcggcgatg aactccatga
    13261 agttgacgac gaactccggc ttgccggtga acctcgaccg cagcagcggg ttctgggtcg
    13321 cgacgcccac ggggcaggtg tccaggtggc agacccgcat catgatgcat ccgctcacga
    13381 ccagcggtgc ggtcgcgaag ccgaactcct cggcccccag cagggcgccg atgatcacgt
    13441 cgcggccggt cttgagctgt ccgtcgacct gcaccaccac gcggtcgcgc atgccgttga
    13501 gcatgagcgt ctgctgcgtc tcggcgagtc ccagctccca cggcgtcccg gcgtgcttga
    13561 gcgagttcag cgggctcgcg ccggtccctc cgtcgtgccc cgcgacgagg atgacgtcgc
    13621 tgagcgcctt cgccacgccc gccgcgaccg cgccgatgcc ggactggctg accagcttcg
    13681 tgtggatgcg ggccttcggg ttcgcgcgct tcagatcgaa gatgagctgc ttgaggtcct
    13741 cgatcgagta gatgtcgtgg tgtggcggag gagagatgag acccacgccc ggcgtcgcgt
    13801 gacgcgtgcg cgccacccac ggatacacct tctgcggcgg cagctgcccg ccctcacccg
    13861 gcttggcgcc ctgggcgagc ttgatctgca gatcggtcgc ctcggtgagg tactggctgg
    13921 tcacgccgaa ccggccggac gccacctgct tgatggcgct gcgacgctcc gggtcgacca
    13981 ggcgctccgg gtcctcgccg ccctcgccgg tgttcgactt ggcgccgagg cggttcatcg
    14041 cgatggcgag cgtctcatgc gcctcctgcg agatcgagcc gtagctcatc gcgccggtgg
    14101 agaaccgctt cacgatcgcg ctgacgggtt cgacctcgtc cagcgggacg ggcggccgct
    14161 cccccgtgcg cagctcgaac aggccgcgga gggtcttcag ctcgtgcgcc tgatcgtcca
    14221 cgagcttggt gtactcgcgg aagatgtcgt accggcgggt gcgggtggag tgctgcagac
    14281 ggaacaccgt gtccgggttg aacaggtgcg gggatccgtc gcgccgccac tggtactcgc
    14341 cgccgatcca cagccgctcg tgggccctgg cggcctcgtc ctccgggtag gcgtagtcgt
    14401 gccgcgcctg gttctcggcg aacacgtcct cgatgccgat gccgccgagc ttggtctcgg
    14461 tgccggtgaa gtaggcgtcg acgagctcct ggctcaggcc gatcgcctcg aacacctgcg
    14521 ctcccgcgta ggagccgacc gtggagatgc ccatcttgga catgatcttc agcacgccct
    14581 tgccgagcgc gtggatcacg ttgtggacgg cctcctcggg gctgatgccc gtgatgtagc
    14641 cggtgcgcac caggtgctcc acggtctcca tggccaggta cgggttcacg gccgaggcgc
    14701 cgtagccgat cagcgtggcc acgtgatgga cctcgcgcac atcgcccgcc tccaccacga
    14761 gaccgacctt catccggttc tcgcggcgga tgaggtggtg gtgcacggcg gcgaccatca
    14821 gcagcgacgg gatcgggacg aggtccttgt tcgagtcgcg gtccgacagg atcagcagct
    14881 cggcgccgcg ctcgatcgcg gcatccgcct cggcgcacat cgccgccagc cggtcagcca
    14941 gggtgtgggg gcccgcgtcg aagtggtaga gaccgcgaat cgtggcgctg gagcggccgg
    15001 gaagagcccg gtcgatgtgc cggatcttcg cgagctcgtc attgtcgatg accgggaagt
    15061 cgaggctcac ggttcgggcg tgatcgggac cccagtcgag caggttgcgc tcgggtccga
    15121 ggcccagacg caggctggtg accacctctt cgcggatcgc gtcgaggggc gggttcgtga
    15181 cctgcgcgaa ctgctgcacg aagtagtcga agagcaggcg cgggcgctcg ctgagcgccg
    15241 cgatcggcgc atcgctgccc atggcaccga gcggctcggt gccgttctgg cccatcgggg
    15301 tgagcaggat gcgcacctcc tcctccgtgt agccgaaggt gcgctggcga cgggtgatgg
    15361 atgccgcggg gtgcacgatg tgctcgcgct cgggaaggtc ggccaggcgc acggcgccct
    15421 cggccagcca gtcggcccag gggtgcaggg cggcgagatc gcgcttgacc tcgtcgtcct
    15481 cgatgatgcg gcgctgcgcc gtgtcgacca ggaacatccg gcccggctgc aggcgcccgc
    15541 ggcgcttgat gcgctccggg gcgaactcca gcacgcccgt ctcgctgccg atcacgacga
    15601 ggccgtcggt ggtctcggtc cagcgcccgg ggcgcagccc gttgcggtcg agggtggcgc
    15661 ccacctgcac gccgtcggtg aagatcaggg cagcggggcc gtcccagggc tccatctggt
    15721 tcgcgtggta ctcgtagaac gcgcgcagat ccgggtcgag atcggtctgc ttctcccacg
    15781 cggcgggcac catcatcatg acagcgtgcg gcaggctgcg accggtcagc gtgagcagtt
    15841 ccagcacctc gtcgaaggat gccgagtcac tggcgccctc ggtgcagatc gggagcagcg
    15901 gggccatgtc gccgagcagc tcggactcga gctgcgactg gcgggcgcgc atccagttgc
    15961 ggttgccgcc gatcgtgttg atctcgccgt tgtgcgcgag catccgcagc ggctgtgcga
    16021 gcggccagga cgggaacgtg ttggtcgagt agcgcgagtg caccacggcg agctcggagg
    16081 cgaaccgctc atcctgcaga tcggggtaga acggctccag ctgcagggtg gtgaccatgc
    16141 ccttgtaccc gagggtgcgg gccgacaggg agacgaagta ggcgccgacc tcgtgtccgg
    16201 cgcgcttgcg cagccggtag gccaggcgat ccagcgcgat cccggagacg gcgccggggg
    16261 cggtgatgaa cagctgctcg aacgccgggc gggcctcatc ggcgagcttg cccaggtgct
    16321 cgttggcggt ggggacgacg cgccagccga ggaccacgag tccttcttcg gtggcgatgc
    16381 gctcgatgcc ggccttctgc tcgcggcggg cgctcgactc cagagggagg aacgcgaggc
    16441 ctgcggcgta ctcgccgacc ggggggaggt cgaagtccgt gacggcccgg aggaacgcgt
    16501 ccggcatctg cgtgaggatg ccggcgccat ctcctgtgcc ggcatccgag ccgatcgcgc
    16561 ctcggtgctc gaggttgcgc agcgcttcca gcgccagcgc gatgatgtcg tggccgggtt
    16621 ccccgcggag cgtcgcgacc atggccaggc cgcaggcatc cttctcgtgg gcggggttgt
    16681 acatcccctg cttcgggggg taggcgccgg aggcgccgta gggaggctgg aagtacacca
    16741 gtcaccgtcc tcagtgtcga aagtgaatcc ggggacagcg tcggcccgct cgttcagtat
    16801 gaaggatcac gcacggcggg agtgctcaga tgacgtcctc gcggtttatg aagccgtgct
    16861 tgtggcgggt gcttcagcgt cgatcccgtc caccggaggc tcactcaggt cgacgtagtc
    16921 gtgggaatcg tgatccgagt ctacatccgc atcggcgggc tctcgcccgg gctggtacgg
    16981 cgacgcctcc agcccggtgt gacggcgcgt ctgcacgatg aggatgacca gaccgacgag
    17041 cacgccgatg atcgccgccc acacgttgct gcgcaggccc aggtagacct ggctcgggtc
    17101 gatccggatc gactcccaga cgatgcgacc ggcgctgtac cagatcaggt agcaggcgaa
    17161 cagccggccc cactgcaggc gcagccgacg gcccagccac agcagcacga gcacgccgag
    17221 cgtgttccag atcacctcgt acaggaacgt ggggtggaac agtgtgccct cgggcaggcc
    17281 tgcagggaac gccgggttgg tggactcgat ctgcaggccc cagggcaggt cggtgggcag
    17341 gccgaacagc tcgtggttga accagttgcc gaagcggccc atggcctgcg cgagcaggag
    17401 ccctggtgcg agggcgtccg cgaaggtcca gaagcggatg cccgtccacc ggcatccgag
    17461 ccaggcgccg acggcgcccg ccatgagcgc tccgaagatg gcgatgccgc cctcccagat
    17521 cgcccacacc gagcccggct cgaaggggtt ccaggtgttc ttgcccgggc cgaagtagaa
    17581 gtcgaagtgc gtcaggacat ggaagatgcg cgctccgatg atggccagcg gcaccgcgat
    17641 gatcgcgatg tcgatgacga cccacttctc cgcaccgcgc ttggtgaggc ggtggttggt
    17701 gatcagcgtc gcagcgatga tccccgcgat gatgcacagc gcgtagtagt ggatcgtgat
    17761 cccgaagatc gggaacgtgc tgaccggagg gctcgggatg ctggcgggca cgcccgagat
    17821 gaggctgtga agcgcaaggg acatgagagt gagtctagtt tcctgtcgcg aatcgcgccg
    17881 acggggtgcc gccggcgaga gaacgggtgg tctcggcgag gccctccagg ccgccgtcgc
    17941 gcagcgcctt gacgagtgcc gtgccgacga tggcgccgtc ggcgtactcg acgacgccgg
    18001 cgatctgctc ggggttggag atgccgatgc ccacgcatgc ggatgccgcg ccgcgggcgc
    18061 gcatgcggtc gacgagcgtc cgcgcagcgc ggtcgagagc agccctctcc cccgtgatgc
    18121 ccatggtgga gaccgtgtag acgaagccgg tggacgagga gatgaccagg tcgaggcgct
    18181 cgtccgtgga ggtcggagct gcgaggaaga tgcggtcgag gccggtgcgg gtgctcgcgg
    18241 cgatccactc gccggcggcc tcgggcgtga tgtcaggggt gatgaggccg gcgcctcccg
    18301 cggcgagcag atcgtcggcg tagcggtcga cgccgtactg cagcaccggg ttccagtagg
    18361 tcatcacgac cacgggcaca tcggtgcggg aggtgatctc cttcaccacc gtgaacaggt
    18421 cgcgggttcg gtagccgttc gccagcgcct gctgcgtggc ctgctggatg acctcgccgt
    18481 ccatcacggg gtcggagtag ggcgggccga gctcgatgac gtcgacgccg ttgcgcgcga
    18541 gggtcaacgc cgcctcgacg ccggtctcca ggtcggggaa gccgatcggc aggtagccga
    18601 tgaacgcgcc cctgcctgcg tcgtgggcat tgcggatggc ggcctcaaca cggctcacga
    18661 cgcgtcctcc tcgtcgtaca ggtcgaagta gcgcgccgca gtgtccatgt ccttgtcgcc
    18721 gcggccggag aggcagacgg cgatcacggc atccgggccg agctcgcggc cgatgcgcag
    18781 tgcgccggcg agggcgtgcg ccgattcgat cgcggggatg atgccctcgg tgcggctgag
    18841 caggcgcagg gcctgcatgg cctcatcgtc ggtggcggga atgtactccg cacgcccgat
    18901 gtcgctcagc caggcgtgct ccgggccgac gccggggtag tcgagaccgg ccgagatcga
    18961 gtgcgactcg acggtctggc cgtcctcgtc ctgcagcacg tacgtcttcg cgccgtggag
    19021 cacgccggga cgaccgcgtt cgatcgaggc cgcgtgcttc ggggtgtcga cgccgtcgcc
    19081 ggcggcctcc actccgtaga gcttcacgcc ctcgtcgtcc aggaacgcgt cgaacatgcc
    19141 gatcgcgttg gagccgccgc cgacgcaggc gaccacggcg tccggaagcc ggccggcctc
    19201 ggcgagcagc tgctcacggg cctcctcgcc gatgatcttc tggaagtcgc ggaccatcgc
    19261 cgggaagggg tgcggaccgg ccgcggtccc gaagatgtag ttggtggtct cgacggtcgc
    19321 cacccagtcg cggtaggcgt cgttgatggc gtccttcagg gtccgggacc cggtcttcac
    19381 cgggacgacc tcggcgccga gcaggcgcat ccgcgcgacg ttcagcgcct gacgctccgt
    19441 gtcgacctcg cccatgtaga tcgtgcagtc caggccgaac agggccgccg cggtcgccgt
    19501 cgccacgccg tgctggccgg cgccggtctc ggcgatcacg cgggtcttgc cgagccgctt
    19561 cgtcagcagg gcctgtccga gcacgttgtt gatcttgtgc gagccggtgt ggttgaggtc
    19621 ctcgcgcttg aggaacacgc gtgcgccgcc ggcgtgctcc gcgaagcgcg gcacctccgt
    19681 gatgggcgac gggcgtccgg cgtaggagtg cagcagcgtc gcgagttcgg tgcggaacgc
    19741 ggggtcgacg atcgctgcct cgtacgcgac cgtgagctcg tcgatagcgg cgatgagcga
    19801 ctccggcatg aaccgcccgc cgtactcgcc gaagaacggt ccgtgctggt cgcgcagcgc
    19861 catgctcact cctctccccc gcgcaccggc aggtcgccgg ccgcgaggaa tgcgttcagg
    19921 gtggcgacgg gttcgccggt gaccagcgcc tcgccgatca agacgacgtc cgcccccgcc
    19981 tcgcggtagc gacgcacgtc ctcgggcgcg agcaccgcgg actccgcgac gcggatcgcg
    20041 gtgtcgggga tgcgctcgga cagggacgcg aacaggtccc gatcgagctc cagggtcttc
    20101 aggttgcgtg cgttcacgcc gatcagctcg gcgcccagat cgatcgcgac ctcgagctcc
    20161 tccgcggagt gggtctcgat cagcggggtc atgcccagct cgcggatcag cgcgtacagc
    20221 tcgcgcagcg tcttcacgtc gagccccgcg acgatgagga gcgccagatc cgctccggcc
    20281 gcgcgggcct cgagcacctg gtacggcgtc gcgatgaagt ccttgcgcag cacggggagg
    20341 tccacgcgtg cggtgacggc ctcgaggtcg gcgagactgc cgccgaagcg gcgctcctcg
    20401 gtcagaacgc tgatggcgga cgcaccgccc tgctcgtaca gcgaagcctg cagcgcggga
    20461 tcggggatct cggcgagagc gccgcgcgac ggactcgcgc gcttgacctc cgcgatgatc
    20521 ttcactcggt cggcgggagt cagcgccgcg agcgcatcgc gggcggcggg gcgcgccaga
    20581 gcttcacgct ccacgtcggc cagcggccga gtcgcagcac gccgctcggc gtcctgcacc
    20641 gcgccggccg tgaggtcggc gaggaccatc agtgcgcctt cggggtgtac ttcggaccct
    20701 tcactcccca gcccgccttc gccatgaccc agccgacgat cgcgccgatg acgacgacga
    20761 cggccgagat ccagacaccc accactcccg aaccgtcggg gaagcagaag aagacagtgc
    20821 cggcggtgaa gccgaccagc atgatcacga cggcagtcca ggctgcaggc gagtgtccgt
    20881 gaccggggtc tgcgatcggg ttggtcatgt tctcctccgg gagggtcgac gatgtcatca
    20941 gtctatcggg aagggccggt cggatccgtg ccatgggaca ggtcgtccca ggaatcgacg
    21001 gcgtcgaggg gcccggcatc cgcgtgccgt ccgtccgaga gctggtcgta gcggcgggtg
    21061 cccgccttcc agcggtgcgc ggtcgccagg acgaagaggg atgcgagcgc caccacgacc
    21121 cacgcgcaca gggcggcgac gccccagccg ctgggctcga ggtcagtcac catcgcgccg
    21181 agcgccttgt caccgctgag gccggtcgcc ttggtgag
//
LOCUS       DATMSX010000495         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_36029,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000495 DATMSX010000000
VERSION     DATMSX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36029"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VN041_15770"
     CDS             complement(<1..785)
                     /locus_tag="VN041_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30539.1"
                     /translation="MNLEEHVQAYADRFGRPVIVFDADLNVTAFSIHDEDVDHARLAI
                     ILSHRGSSRAKDSIREYRVAQADGPVRIPPIDGGRSRVVAPVRHEGHVVGYVSSAIPD
                     DLPDDAIVEDDAIRESRAQIGVILAAMALGTREDEDRTRRLVTALLDADGDHGIRAAD
                     ELLTDRLISPAPHYTVIVLSPPPHSDVNTATLRLALDRALAAIPAFPTLKAVGTVFEG
                     EAVLVVPYEIDGKRLSTLLALPAFAELRAGVGGAHSPLADARAS"
     gene            947..2566
                     /locus_tag="VN041_15775"
     CDS             947..2566
                     /locus_tag="VN041_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWU30540.1"
                     /translation="MAKMTGGDALTQALISEGTETVFGIPGVQLDGLTDGMYRRQDEL
                     NLLVPRHEQSTTYMADGFHRVTGKPGVAMVVPGPGVLNAGAGMATAYACSSQVMLLAG
                     TIPSKLVGGGLGALHEIPKQSETIEGLTKWTARPTRVEEIPDLVHEGFRQMRTGRPRP
                     VALELGPDILHAEADFEVGEPFAVETPSAPEDGVSEILALLAASRRPVIHVGGGVRGA
                     RAFDLLPRLAELLDAPVVMSENGRGAIDARDPHALPAFSLWELRDTADAVVSFGSRFL
                     TPFGQQLNIGEARLALVNVDPDDVRAPRRPDVTVLADATAVLEALVARLEGQEHRTGW
                     GDELARLRRECEERIRAQVAPQMAFVDAIRASLPADGIFVNELTQIGYVSTYGFPVQA
                     PRSYVWPGYQGTLGYAMPTALGAAVGAGDRPVVVVTGDGGIGWSLQEFATAKRYGIPL
                     ITVLFEDNRFGNVWRIQRDTYGGRFIGSDLVNPDFELLVKAFGIDFALAEDADAVQRA
                     IGEAVTARRPAVIKVPVDVFPSPWPLIHEKH"
     gene            complement(2604..>3225)
                     /locus_tag="VN041_15780"
     CDS             complement(2604..>3225)
                     /locus_tag="VN041_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU30541.1"
                     /translation="RVIGRGGHGSRPHQAVDPVPALAEIVLALQTFATRRFSPFDPVV
                     ITVTKLHASDIVNAIPDEATLEATVRTLSPHTTTVLAQELPALVEGIAAAHGCRGEAV
                     FRAVYPVTTNDADAARHALSVLEQTFGSDRVEELAHPAMASEDFSYVLDEVPGAFVFL
                     GATPAGTDPEEAEMNHSPRAVFDDGILGDQAAALAALALSHVGSTR"
BASE COUNT          453 a         1108 c         1184 g          480 t
ORIGIN      
        1 gaggcgcgcg catcggcgag aggggagtgg gcgccgccca cgccggcgcg cagctcggcg
       61 aacgccggca gggcgagcag agtgctgagg cgcttgccgt cgatctcgta gggcaccacg
      121 agaaccgcct cgccctcgaa gacggtgccg acggccttca gggtggggaa ggccggaatg
      181 gcggccaggg cgcggtcgag tgccagccgg agggtggccg tgttgacgtc cgagtgcggc
      241 ggaggcgaca gcacgatgac ggtgtagtgc ggggcgggcg agatgaggcg gtccgtcagc
      301 agctcgtccg cggcccggat gccgtggtcg ccgtcggcgt cgagcagagc ggtcaccagg
      361 cgacgtgtgc ggtcctcgtc ctcgcgggtg cccagcgcca tcgcggccag gatcacgccg
      421 atctgggcgc ggctctcgcg gatggcgtcg tcctcgacga tcgcgtcatc tggcaggtcg
      481 tcagggatcg ccgaggagac gtagccgacg acgtgcccct cgtggcgcac gggtgcgacg
      541 acacgcgagc gtccgccgtc gatcggcggg atgcgcaccg ggccgtcggc ctgcgcgacc
      601 cggtactcgc ggatcgagtc cttcgcgcga gaggatccgc ggtgggagag gatgatcgcg
      661 agccgcgcgt gatcgacgtc ctcgtcgtgg atgctgaacg cggtgacgtt gaggtcggcg
      721 tcgaagacga tgaccggcct tccgaagcgg tcggcgtacg cctggacgtg ctcttcgaga
      781 ttcaccggtc gtctccctct gcgcgtgacg gtccgtgcgc cggctctgcg acaccgtgtc
      841 cgatcgcacc gattctatgc cgcgcgccct cccggatccg gccgagttgt ggcgccgagc
      901 acgctgatct atattcatta tagtcaattc aggaaggcgt tcacctatgg cgaagatgac
      961 gggcggcgat gcgctcactc aggctctgat cagcgagggg acggagacgg tcttcggtat
     1021 ccccggcgtg cagctggacg gcctcaccga cggcatgtac cggaggcagg acgagctgaa
     1081 cctgctggtc cctcgccacg agcagagcac cacctacatg gcggacggat tccaccgcgt
     1141 caccggcaag ccgggcgtcg cgatggtcgt ccccggtccg ggcgtcctca acgccggcgc
     1201 cgggatggcg acggcctacg cctgctcctc gcaggtgatg ctgctggccg gcacgatccc
     1261 cagcaaactc gtcggagggg gactgggcgc actgcacgag atcccgaagc agagcgagac
     1321 gatcgagggc ctcaccaagt ggaccgcgag gcccacgcgc gtcgaggaga tccccgacct
     1381 ggtgcacgag ggcttccgcc agatgcgcac cggtcgtccg cgacccgtcg cgctcgagct
     1441 cggccccgac atcctccacg ccgaggccga cttcgaggtg ggggagccgt tcgccgtcga
     1501 gacgccgagc gcgcccgagg acggcgtgtc cgagatcctc gcactgctgg cggcgagccg
     1561 gcgccccgtc atccacgtgg gcggcggcgt gcgcggagct cgcgccttcg acctgctgcc
     1621 gcgtctcgcg gagctgctcg acgctccggt cgtgatgagc gagaacggcc gcggcgcgat
     1681 cgacgcccgc gacccgcacg cgctgcccgc cttctccctc tgggagctgc gcgacacggc
     1741 ggatgcggtc gtctccttcg gcagccgttt cctcactccg ttcgggcagc agctgaacat
     1801 cggcgaggcg cgcctcgcgc tggtgaacgt cgatcccgat gacgtgcgag cccctcgccg
     1861 tcccgacgtg accgtgctcg ccgacgcgac cgcggtgctc gaggcgctcg tggctcggct
     1921 ggagggacag gagcaccgca cgggctgggg cgacgagctg gcgcgtctcc gccgcgagtg
     1981 cgaggagcgc atccgcgctc aggtcgcgcc gcagatggcg ttcgtggacg ccatccgtgc
     2041 ctcgctgccg gccgacggca tcttcgtcaa cgagctcacc cagatcggct acgtgtcgac
     2101 ctacggattc ccggtgcagg cgccgcgttc ctacgtctgg cccgggtatc agggcacgct
     2161 cggctatgcc atgcccaccg cgctcggcgc ggccgttggt gcgggcgatc ggccggtcgt
     2221 ggtcgtcacg ggcgacggcg gcatcggctg gtctctgcag gagttcgcga ccgcgaagcg
     2281 gtacgggatc cccctgatca ccgtgctctt cgaggacaac cgcttcggca acgtctggcg
     2341 catccagcgg gacacctacg gcggccggtt catcggctcc gacctcgtca atcccgactt
     2401 cgagctgctc gtgaaggcct tcggaatcga cttcgcgctc gccgaggatg cggacgcggt
     2461 gcagcgcgcg atcggcgagg ccgtgaccgc acgccgcccg gctgtcatca aggtgccggt
     2521 cgacgtgttc ccgtcgccct ggcccctcat ccacgagaag cactgactct ccgcgggccg
     2581 gcctcgtgcc ggcccgccgt cagtcagcgc gtgctgccga cgtggctcag cgcgagcgcg
     2641 gcgagcgccg cggcctggtc gccgaggatg ccgtcgtcga acaccgcccg cggtgagtgg
     2701 ttcatctcgg cctcctcggg gtcggttccc gccggggtgg ctccgaggaa cacgaacgcc
     2761 ccgggaacct cgtcgagcac gtacgagaag tcctcggagg ccatcgccgg atgggcgagc
     2821 tcctcgacgc ggtcggatcc gaaggtctgc tcgagcacgg acagcgcgtg gcgcgcggca
     2881 tccgcatcgt tcgtcgtgac ggggtagacc gcacggaaga ccgcttcgcc gcggcagccg
     2941 tgcgcggcgg cgatgccctc gaccagcgcc gggagctcct gcgcgagcac ggtggtggtg
     3001 tgcggggaga gcgtgcgcac cgtcgcctcc agcgtcgcct cgtccgggat cgcgttgacg
     3061 atgtcgctcg cgtgcagctt cgtcacggtg atgaccaccg ggtcgaaggg ggagaagcgg
     3121 cgggtggcga acgtctggag ggccagcacg atctccgcga gggcgggcac cgggtcgacg
     3181 gcctggtggg gacgcgagcc gtgcccgccg cgaccgatca cgcgc
//
LOCUS       DATMSX010000496        31565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149306,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000496 DATMSX010000000
VERSION     DATMSX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 31565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31565
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149306"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VN041_15785"
     CDS             complement(<1..348)
                     /locus_tag="VN041_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWU30542.1"
                     /translation="MRILPRLRPRTRVVRAIVSGALLLLVVSAVALSLGDRATNPVDL
                     WHALQGTGAENIVYAVQEVRLPRVALAVLVGAAFGMAGGLSQGVLRNPLASPDVLGIS
                     AGASVGAVAVIAAG"
     gene            complement(345..1367)
                     /locus_tag="VN041_15790"
     CDS             complement(345..1367)
                     /locus_tag="VN041_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWU30543.1"
                     /translation="MTETKLRPVRRAPLIGALVTASALLLLAAVLSLLIGARVLSVRE
                     MLGGLTGTDHVASAIIWQLRLPRTVVGLVVGAALGAAGVLAQAVTRNPLADPGLLGIS
                     AGAAVAIVAGSVMFGVGGGVPRVLLAIAGAAVATALVYLFAHRAPDGLTPVNMTLAGM
                     AVTACLGGVVSGIVLFSASTMDQYRFWVVGALSIPHANVLIPAGAAVLVGAGIALVVA
                     GALDALALGDDAAAALGVHVVRTRLLTGVAVVLLSGAAVAVSGPIGFVGLIVPHAVRM
                     LVGGDVRRALALSAIVGPLLLLTADVIGRVVVRPAELQVGVVTALVGTPVFIWLTRRT
                     RAGSRS"
     gene            complement(1376..2443)
                     /locus_tag="VN041_15795"
     CDS             complement(1376..2443)
                     /locus_tag="VN041_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HWU30544.1"
                     /translation="MPLSPRLAAPLIAILAVAVALLGCAADAESTGGGGAAANKSNVA
                     TGQEGFSDVRKLAAQLGTDAKDGEFPRDVRNIVANGKVGITTIPERPERVIVLDTGEL
                     DNMLALGVKPIGIAHPAEAAIAPDYLDAGDAVDLGPYDALDLEQIKNLEPDLIIGSSL
                     RIDDDGLKKKLQEIAPTVLSPRPGVLWKEDFLLFAAAVGEKAKAQSLLDAYEERAAEI
                     GEKVVEANGGKAPTISMLRFMAGRIRLYANDSFIGIILADAGLPRTETEDVDELAVEI
                     SAEEITRAEGDWLLWGTYGDAAATEQAAVVDGPLWGQVKAVADDGVVKQVSDETWYLG
                     LGVEGAQLVLDDLEAIAAGRR"
     gene            2635..3543
                     /locus_tag="VN041_15800"
     CDS             2635..3543
                     /locus_tag="VN041_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWU30545.1"
                     /translation="MSRRSSGILAVAVTSVLWGTTGTAATFAPSASPLAIGSAALGIG
                     GLLQFAVAWHAVTGGRTRLAQHRGIILAAGLAVAVYPLAFYASMHLAGVAIGSVVSLA
                     SAPLFSGALEAVIDRHRPDGRWTAAVVLAVGGGAALILSHEVGSGSNVVLGVALGLVA
                     GATYALYSWAARRLMDAGIERAAAMGAIFGLGGLLLMPVLFVTGAPLVASPQAFAVSA
                     YMALVPMFLGYLLFGFGLARIPASTATTVTLLEPAVAALLAVLVVGERLSLVGWVGMA
                     AIAASLLVLSLPARAGGAGDRSAPMP"
     gene            complement(3584..4321)
                     /locus_tag="VN041_15805"
     CDS             complement(3584..4321)
                     /locus_tag="VN041_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HWU30546.1"
                     /translation="MARSSHRRCPRRLTVVAVSWVIGAAAALCAIGGLVMLWWFGRDD
                     PRQYEGQVDAVEKRFADGHPQGGVLLSGSSFFEYWEDSTADLAPLHTTNIGIGGTKVA
                     DHLAHFDRMVVPFHPRALVLYIGSNDISGIPLFTKSAQETVSLIDEYIARVRAELPDT
                     RIYYVAITEAPSRARVRDEIRAANRMLAERAERSGDFVFIDTAPALLTADGSIDGSLF
                     RADRLHFNEKGYARFAAAVRAGLAPEY"
     gene            complement(4431..6929)
                     /locus_tag="VN041_15810"
     CDS             complement(4431..6929)
                     /locus_tag="VN041_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HWU30547.1"
                     /translation="MTENSRAVFISPPFAQGADEEPASYLRKEFTVATRPARAVLRTT
                     AVGLIEPHLNGAVVGDEVLAPGWTSYRHRLIVSTHDVTDRIAVGVNVVGAVLGEGWAV
                     GRLGWEGKRRHYSDRPAAWFELELQYESGTTEFVVSDTGVVAARGGVRANSIYDGEVF
                     DAREELEGWDRAGFGAAEWVRVDSFDWPLASLAEPIAPPIRRIEELAPQQVITTPSGR
                     LVLDFGQNISGWVRLTVDGERGTTITLRHVEVMVDGEIEAETLRTAAATDRYILRGGA
                     AETWEPRFTFHGFRYVEVDGWPGDFSADAVRAVVVHSDMERIGWLETSDPLLNQLHSN
                     AVWSMRDNFVGVPTDCPQRDERLGWTGDLNAFAPAAAFLYDVRGVLGSWLRDLAAEQR
                     EKGFVPWVVPDVLSTPSSPTALWSDVAVSLPCVLHREYGDEDILRDAYPSMTTFIRQV
                     ADLLDENGLWSTGFQYGDWLDPDAPIDNPAGGKTDRALVASAYLCKTTQEMADTAQIL
                     GHEEDAVEFGALAKRVRDAFRSEYVTPMGRVAGETVTGYALALAFGILDEAQRAHAGD
                     LLAQLVAKAGYRISTGFAGTPLVADALSGTGHLAEAYLLLQETGCPSFLYPVTMGATT
                     IWERWDSVRPDGTVNPSGMTSLNHYALGAVIDWMHRSIGGLTAIEPGYRRMRIAPLPG
                     GGLTWARLRHVTVQGMVEIAWEISGGTVSLTVDIPENASAEVILPLHPDGETHHIASG
                     VHTWSYELPAGAGPRRYSLDSTLKDLADAPAAWRAFTEAFAAHFPGVPLDGTAPEAAF
                     MTVATMLEYVPGASDELRNDLASALEAVSEGAPA"
     gene            complement(6958..8301)
                     /locus_tag="VN041_15815"
     CDS             complement(6958..8301)
                     /locus_tag="VN041_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30548.1"
                     /translation="MNNPTDAEPGPAPLPAVMPQPPADPAALVHGEEQANPDLPKVDA
                     RYIWFMVLAQFGVFMAFITPLAISLTIRVNTLAPGHPEYLGFITGAGALFVMLSSPFM
                     GIWSDRTRSRIGRRRPFMIGGMLVGVLSLVVMAIAPNAVVVGLGWILAQWGWGTTLSN
                     LQISTADRLPESQRGKVAGLTSFATQIAPVFGVILAQFFTGDALLLFLVPGAVGVLFV
                     LLFVTLVHEDDSRGLPKDPITVVQVLAKYLYRPRRYADFSWNWLGRFFFYTGITLNTT
                     YTAYFFADRLGITVESVAGIMAGLSLAGILAVTAGAIGGGFLSDRLRRRRAFVLLGGV
                     VMAAGMVMQAFAPDLTLLIVASLIGSVGLGLFAAVDQALLLDVLPERETDAGRFMGIT
                     GFATSIPQSVMPLVASGLLLVGVSGGARNYPLVFIVGAVAVVLGGASVLRIRSVR"
     gene            8488..9465
                     /locus_tag="VN041_15820"
     CDS             8488..9465
                     /locus_tag="VN041_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30549.1"
                     /translation="MTAADVAHALGISRATVGFVLNNTPGQKISAVTRERVLAEAKRL
                     GYRPHTAARALRSGQSRIVLLILPDWPMDYSMRTHLDEASLVLDRAGYSLVTMTPHPG
                     GQAQPLWETLRPDVVLGMAPLPREMTSEIRESGVQHVLAAVLEDQPEPAQGDLGFVEG
                     PRLQVEHLLSTGRRMLAFAGSADPRIADLVRIRRTLAGRTLTERTGTGFAADADIDQG
                     NAAEVLEDWLSRGVDGVVAYNDDIAALVAGSVLRSGRSVPGDVAVVGHDDSPLASLFV
                     PGLTSVRIDTAGLGRYVAELALSAVTDSPKPEAGPETEAHLVIRQSTGG"
     gene            complement(9836..10873)
                     /locus_tag="VN041_15825"
     CDS             complement(9836..10873)
                     /locus_tag="VN041_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWU30550.1"
                     /translation="MSTALSTQIQLAKRPVGWPTLDDFQTTQVVYEAPSAGEVRVRNE
                     FVSVDPYMRGRMNDVRSYVAPFALGETITGGAIGRVIVSEAPELPVGTLVLHQHGWSD
                     VVQADASTFRAVPDVPGLPSSLRLHILGMTGLTAYVGLTAIAGLKPGDTVFVSGAAGA
                     VGTAVGQIAKLLGAGRVIGSAGTPEKVALLTEKYGYDAAFNYRDGDVRAQLAAAAPEG
                     IDVFFDNVGGDHLEAALDAFNDGGRAALCGAIAGYNTTDRTPGPDNMANIITRALTLK
                     GFTLAAHLGLAPEFQEKMTAWFAEGSIAYDETIVDGIDNTVHAFLEMMRGANTGKMLV
                     RITTDEGDARA"
     gene            complement(10907..11293)
                     /locus_tag="VN041_15830"
     CDS             complement(10907..11293)
                     /locus_tag="VN041_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HWU30551.1"
                     /translation="MVVAGLILAGLAALVHVYIFVMESLIWTTPRVRATFGVTEEEAL
                     ATKSMAFNQGFYNLFLAISVFTGIVLFAVGAHAVGATLVFVGAGSMAAAAIVLLLSSP
                     DKRGAALKQGVIPAVAVIILALGVTL"
     gene            11373..11957
                     /locus_tag="VN041_15835"
     CDS             11373..11957
                     /locus_tag="VN041_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30552.1"
                     /translation="MARTPSFDREAVVRAARTLFWTVGYESASIPDLEAATGLSRSSI
                     YNAFGSKRGLFDAAVQSYLDEVVRPRLRPLSGEVVDPEAIIAYLQGLRDAFGTLTSMP
                     ASHGCLLVNAAGAPIAHDPKVAQVISDYRAELHDAFARGVDARTPDLDAAERERRVTA
                     ITSSVVAAFALARIDPAEAGRGIASAIELVARGA"
     gene            complement(12501..13838)
                     /locus_tag="VN041_15840"
     CDS             complement(12501..13838)
                     /locus_tag="VN041_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30553.1"
                     /translation="MAVLPKDEFKRLVTGLTDDQYLRVLEALARADVDLPGWRDRSLV
                     SEDQLCASDPVSVHVPFVAWYRALELARRVATTERGVWIVTDADSVRVVGADNRAAAC
                     SSITATVSVPGIALVDGVEAFLVTDALAKGMTAAVRRSASITLHAEDNLHLRLSVNGR
                     SHRARLHRRGEESPSMPPVAEDEVVVGDAAALRRAIFHVCPASDSRGSDDVLRLDRVG
                     MRAVDGVLTIDATDRYRAAEANLPVSHEHAFEAFVDATWLREVSDALTHGHVQIGISQ
                     EHGTSLLMIAGDRWSAWTTAVGAWRRRYHPKLAAQRVSATVARNDLQRFLRDAHRLAT
                     AGSRGPSDGCVRATVEPGTLRISGMNDDDESANIGSVRLPADTAGLTRTTVVYLDIKR
                     TIDALRSFANEVVTVHLDDDRMVHVTPSESTPDGQPALMWSMVGSLPPDERDI"
     gene            14387..14938
                     /locus_tag="VN041_15845"
     CDS             14387..14938
                     /locus_tag="VN041_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWU30554.1"
                     /translation="MTARTELMAIAAEQDGYVTLDDARALGIAERDLQQLAHRGKIIR
                     EATGVYRFEEFPVTRAASYRFAVLWTGRPEAALSHDTALSLLELSDINPPKTHVTVGT
                     GERIRRAGGVGIVVHKEDLAPKDLSWWEGIRCVKPFTAIRQAIDTHVPFQFVNQAITE
                     ARARGSITDAERVTLRSRLERER"
     gene            14935..15891
                     /locus_tag="VN041_15850"
     CDS             14935..15891
                     /locus_tag="VN041_15850"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWU30555.1"
                     /translation="MSDPFSALPEKSRTPTSASILNQWVSHAEQIVGARGDRTRWVLA
                     STIVAAALQRALMADDTPLFVVKGGVFIERALNLQSRATKDLDTLFRGAVEEFEQAAD
                     DALDEPWGMFDLTRTAFTVIEGAKTSRKPRRFNVLLDIKGKRWANIKVEVSFPEGSID
                     KHVGTIPAPSTTFFGIEQPSDLATITMAYQVAQKLHACTDPHNPPDESNLRVRDLVDL
                     YLIKQTFYPDDADLTEVRSAAVDVFSARAEEAKAVGSPSRTWPPVIVSNELWESEWAK
                     PAGEAGVVLSLGDVLEALNKWVAAIEQAEGASTGSVDELTEQ"
     gene            16085..16504
                     /locus_tag="VN041_15855"
     CDS             16085..16504
                     /locus_tag="VN041_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30556.1"
                     /translation="MAKTRSLFVPYVTDRVMPEAVEYLPLGHDARLTAAKVSAFVEET
                     DSLPDVRLAVLGVLRAHSMNGAETPTAVISVVFAALAVLISVVATIGGFGSAVGYAFG
                     VGLVVLGGVFTRVAFTAHVRRMTCGVWLAAYEDALRR"
     gene            complement(16514..18460)
                     /locus_tag="VN041_15860"
     CDS             complement(16514..18460)
                     /locus_tag="VN041_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30557.1"
                     /translation="MKQTVFDFLDTLEGAPSVANLGGEHTIYASSDALAGDTRVVEVA
                     TALCAEWGDLQDQKSACALLISAASAIPALEVRALYDVLLAAETALPQIAPGLEGAFR
                     ARAAGRGFERDIALEGWTRLALGRWAKNTLQLRASLHEGSEAADATPPLVRALGAACS
                     MWGDAELLGALTALTTHDDLDSDAAMELGFRKIASAAASTDVEAVRSTLDDALRWFDT
                     AYHDNARPDAAAFKAVITGVTEQVAGHSISEERFDAISGAVYEYLDGYRGTQPDWRGA
                     RAGTTDAWLDLLAQLRVADADGWFDTETTVRALAHVFAAEQTMVLVVNPGMTEQASQL
                     GVRELVWPRVEEIAQGNSSFITHMQRWLLVSAEPEGSDTRAAVESLLARLQERPPKKA
                     GGESIRLPDVIRENLRLTDDAFSELEIAAASAPSLIPVFEQVAFRMGSLIIAENDLLT
                     TLLAQCDEDAEGGIGTYRTELTTLLIDLIRYSSHHLNISQSPSRLPPWMAKGQPWPEE
                     HQLADDLNVALLMAGRDAAVERPNVAGGRVDIAVTFPGRCTIYIEVKTIDQDRTDEQL
                     VDDFGTQSVQYAATNIPVAILVVADYVPRKIRRDLTGTFRVTPVRLDPTSRQHALVGV
                     RLQANVEPPSATSAKRITRTKPSP"
     gene            complement(18474..20630)
                     /locus_tag="VN041_15865"
     CDS             complement(18474..20630)
                     /locus_tag="VN041_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30558.1"
                     /translation="MEATAVDWVDTVAERVEADRDVVAEVLTRYGVVGPKPVPTRRPL
                     RVDALHFAGVKNLADDVDGETRQFVPFSFYRAFTTPVTAFVSDGKNDAGKSSTLDILK
                     WGLRGSARVAGDVQKWLRQACLLLTIGGERVLIAWRVDDGNPRGQILILPSGVGADLD
                     DFNFLSSAAMTELSEEAEVAGEPVFELNVPVDDLRDRLLADGALLVDTFDGHDEMELV
                     VDDFMLQRLGLDALTQWTRVANATDADDGNMVDHGWHLWSQALAITKPSESSVIGETP
                     FQARNVLNTFLSTDWGATSLSARVRRKGVDAQLSGIARRRNRDTEAREASTLALRAER
                     AEAAAELESIPVSGMTAEEVEAAMAAVTAATTEITRTQRAMSDAALAYGDAERDLDAA
                     ERDLVAFKEAAVTKRFWHALKPSCCPRCDAKVQPEQWQREQEGSCSLCNNPIDDVVDE
                     AHGAPHDDDAAEEVNDDNLDPLALAEERVAQHTADVHRFSTLHDEAMAQAKAAHDEFE
                     RASEVMRNVHGDPIERRALERRIAVLDGRIQERSRPSEVEPEEAALTTDSQILEAAEV
                     LAAASAKSDFAEALTSVSAQITTLGKTLGISNLEKATLQANAQLPVIKGGEKTAFGKL
                     TDGEKLRLKIALVVALLDVGTASGMGRHPGLLIVDSLATEELNRDNGELLLRELDRVA
                     ETHGLQVITSTAHSELVAAVLPDEAVQRPTAGDLMW"
     gene            complement(20630..21301)
                     /locus_tag="VN041_15870"
     CDS             complement(20630..21301)
                     /locus_tag="VN041_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30559.1"
                     /translation="MQAVSSDARRRGGSTSQTATRVLILLDELGLAVPASCDVEDAVT
                     VVSSLIRLQKLDFWLRNPDYLADELLTEFESGRVTLDQIKPHVVRMLEGDAPQLHTYP
                     MERYIYGAYEFIDDALAVLKLYGQIEHRRAADSGPMSRRDYFLLQKGRDTVAAMRTEI
                     PELDWYVQQASAIGLIADAAQGASAKRRQYLQTEYAGTAHGDVIPSILDRVRERAVAL
                     KVMEG"
     gene            complement(22188..22279)
                     /locus_tag="VN041_15875"
     tRNA            complement(22188..22279)
                     /locus_tag="VN041_15875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22241..22243),aa:Ser,seq:gct)
     gene            complement(22368..23630)
                     /locus_tag="VN041_15880"
     CDS             complement(22368..23630)
                     /locus_tag="VN041_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWU30560.1"
                     /translation="MSESPRRRTPVARHGQLRTPGATGQLFKLLGIALAVILVSGLGV
                     GTFVVGGLLNTVSANAVELENQKELPPDISAYQGGFDILLTGVDTCEPKYAYLFEKRC
                     TGPDSKGTLNDVNLLVHVSAAPRRVTVVSFPRDLMIPIPSCTDANGKTTSAMSKQQLN
                     VAYEHGGDKGGLNCVAKTISSLVGDEDIEFAAKVTFGGVIEITDAIGGVDVCLANPIK
                     DRFTGLDMSAGDHTIQGLQALQFLRTRHGVGDGSDLGRIGNQQQYMSSLVRKLVSGQV
                     LGNVGTMLKLADVGLNNLEASSSLANPMTIAQIALAVKDVPFEDIVFVQYPTVYDPAD
                     TDRVIPDKRNAKILWDAITANKQLQITHENTKNDGVVVKEPDAGTTAPPATPTPTGTP
                     ADVVELPSSITGTSAAQVTCSNGNVRGR"
     gene            complement(23652..23736)
                     /locus_tag="VN041_15885"
     tRNA            complement(23652..23736)
                     /locus_tag="VN041_15885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23700..23702),aa:Ser,seq:tga)
     gene            complement(23853..24647)
                     /locus_tag="VN041_15890"
     CDS             complement(23853..24647)
                     /locus_tag="VN041_15890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWU30561.1"
                     /translation="MSSRGLVGLDIDGTILLQDETLSPGVTEAVTEVRDAGYDVMLAT
                     GRSWSGTRRYVEVLGLSAEFTVCSNGAVIMRRVGDEWVRWHVEVFDPAPVLALLRDRL
                     PEARYMVELESGQRLYTAVLDDWTLDGGREVDFDELISQPVSRVVVVSPEHDEDDFHQ
                     LVTEVGLNEVSYAIGWTAWLDIAPQGVDKSTALERVRAELGIPRDRVLVAGDGRNDLG
                     MFAWALAGEGRAVAMGQAPDEVKDAAGEETTVVDDGGLAIALRTLL"
     gene            complement(24644..25954)
                     /gene="serS"
                     /locus_tag="VN041_15895"
     CDS             complement(24644..25954)
                     /gene="serS"
                     /locus_tag="VN041_15895"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWU30562.1"
                     /translation="MIDLTLLRDEPELVRRSQLARGNAPETVDAAIEADRSRRDALNA
                     FESLRAEQNAFGKTVAKAPKEEKAALVAQAKDLAERVKAAQQASNEAADAAASALAKI
                     ENVIIDGVPAGGEEDFVELRRVGDVPAFDFEPRDHLEIGELLGAIDMERGVKVSGSRF
                     YFLKGIGARLEIALMNLALDKALQNGFTPMIVPTLVRPEIMQGTGFLGEHADEVYHLD
                     KEDLYLVGTSEVPLAGFHKDEILDMSSGAHRYAGWSTCYRSEAGSYGKDTRGIIRVHQ
                     FNKLEMFVYTTPEDAEAEHDRLVALQEEMLTALGLAYRVIDVAAGDLGSSAARKFDIE
                     AWVPTQGAFRELTSTSNCTTYQARRLDVRYRPESDDASQAKTRNVATLNGTLATTRWI
                     VAILETHQQADGSVTVPEVLRPYLGGIEVLRPLGWVDPAAQAAE"
     gene            26057..27055
                     /locus_tag="VN041_15900"
     CDS             26057..27055
                     /locus_tag="VN041_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWU30563.1"
                     /translation="MSASGNARTHAALVLNPVKVDARRLRSTLTALSKKHGWAPPRFY
                     ETSIDDPGRIITRTALDEGAAAVLAAGGDGTVRSVSEAMAGTGVPFAIAPGGTGNLFA
                     RNLGLPLLDPEKVMEAVFTGFTHPVDIGWASLTRENGTETEHGFVVLAGMGLDADMIA
                     NTRPDLKKTVGWIAYLDGAARSLPGAKPFRVVYQLDDGRLHSTKVHSMLFANCGALPA
                     GIALMPDASLDDGTMDIAVIQPTGPLGWLGIWRKVWWDNSVLRRSRAGRRVLERRGRN
                     SSIRYLRGRTADTAMVSPTSIELDGDELGTAVRMSCRIEQGGLSIVLPKGHDVSNF"
     gene            complement(27065..27802)
                     /locus_tag="VN041_15905"
     CDS             complement(27065..27802)
                     /locus_tag="VN041_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30564.1"
                     /translation="MSLPRLRLLPSMLALTAVAFLAAGCSTTPTSSPPTSTGGTDGTA
                     PSQSKDPSGEDVEAAWLAGGASIGIVTWGSSSKDCQPSQAEVKADGQTLQVTLKDRAD
                     GKTPCTADFGPRAIAFPVPAGVDVTKDVEVDVTYGELKADADLDALPSSPNDTGMGQP
                     SAGWFDDQGILLMTYGSSSCPPMPEQVLQTKDQISVSFATIDRVCTMDMAPRVTVIQL
                     PQEREGDGAFTLKLSGDHLDAEVPVIG"
     gene            complement(27845..29299)
                     /gene="cls"
                     /locus_tag="VN041_15910"
     CDS             complement(27845..29299)
                     /gene="cls"
                     /locus_tag="VN041_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HWU30565.1"
                     /translation="MNNWPWWAVLGYLVIDITVRVVSIIVVPRNRRPTSAMAWLLAIF
                     LIPVVGVLLFLLIGNPKLPRARRRKQEQVNEYIAEAASHLNFGSLRPNAPEWFPPLVA
                     MNQHLGALPLSGGNEAHLISDYQQSLDDMADAMREAEHYVHVEFYILQSDASTDNFFR
                     AMEEVAARGIPVRVLLDYWANRWKPKYRETLKRLDAMGADWHLMLPIQPLRGRFQRPD
                     LRNHRKLLVVDGSVGFLGSQNVTDSSYNLPKNIKRGLHWVDLMVRVKGPVVASLNAVF
                     LSDYYAETDRVPDAGELTRVEHGAGELGCQIVPSGPGFEVENNLRLFLALLYAAKERI
                     MIVSPYFVPDEALLLAVTAAVDRGVQVELFVSEEGDQAVVYHAQRSYYEALLRAGIRI
                     WMYPKPYILHTKSLTIDDQVAVIGSSNMDMRSFGLNMEVSMLVRGEEFVDEVRVVEDE
                     YRSLSRELTLEEWDKQPLRSTVLDNLARLTSALQ"
     gene            complement(29404..29580)
                     /locus_tag="VN041_15915"
     CDS             complement(29404..29580)
                     /locus_tag="VN041_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30566.1"
                     /translation="MDDIWMAVGWAILPTLAVSAVFVIVLRGILRFDRTERKVHARIE
                     AEERAARGLPPKAS"
     gene            29732..30844
                     /locus_tag="VN041_15920"
     CDS             29732..30844
                     /locus_tag="VN041_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 family protein"
                     /protein_id="HWU30567.1"
                     /translation="MTTIIHATEPAELLGIVPALAGFTPRRSVVLLPFRGARTHGALR
                     VDLPAPDADPEAFADAAVHALLQVSGVDAAALVVYTDDHPLPVPDGVLLPQLVLAEAL
                     GDVILEAGLHLVDALCVTPDGWGDYREDDPAIQPLESIPVGHGIPGVGDVRGDQLAGA
                     ELPQSDLADRERVGRALTELEELLDRRPSTSPARDANPIALITAMEVLDDLPGFVEEL
                     IEQPAEDDPYCCAALLWCLARPALRDAILVQWATDREFGCRALEAQMEFSRQGGDIPR
                     TIGEVFLGRGARPDPDRLGCALELVRLAAARAPRQTKAGALTAAAWLAWALGRSSHAG
                     VYVDEALRIDPEHSMASLISTMLSAAVLPEWVLRRG"
     gene            complement(30856..>31565)
                     /locus_tag="VN041_15925"
     CDS             complement(30856..>31565)
                     /locus_tag="VN041_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWU30568.1"
                     /translation="RALVFGSVPLPTVQGPDSPAAPPPDPNGIDLSTPGLKAAQASVA
                     KISGVASQCSIISTGSGFVIAPDRIMTNAHVVAGVTSPVVELPGEPARDGRVVYFDPV
                     DDIAVVAADVTAKPLGLSDALAPGAGGAVMGYPFGGPFRVVPAGVLSSSTASIQSIDG
                     SSSDERSIYALQAQVEPGNSGGPLLTQDGQVAGMVFAKDEVKQNIGYAMTNAELLPVI
                     AQAENATKGVATGSCRR"
BASE COUNT         5150 a        10729 c        10529 g         5157 t
ORIGIN      
        1 gccggcggcg atcaccgcga ctgctccgac cgaggcaccc gcgctgattc ccagcacgtc
       61 ggggctggcg agcggattgc gcagcactcc ctggctgagg ccgcccgcca tcccgaaagc
      121 cgcgcccacg agcacggcga gcgcaaccct gggcaggcgc acctcctgca cggcgtagac
      181 gatgttctcg gccccggtgc cctgcagggc gtgccacagg tcgacaggat tcgtcgcccg
      241 atcgccgagg ctcagcgcca cggcactcac gacgagcagc agcagcgcgc cggagacgat
      301 cgcccggacc acacgggtcc gcgggcgcag tctgggcagt attctcacga ccgactccct
      361 gcacgcgtgc ggcgcgtgag ccagatgaac accggagtgc ccacgagtgc ggtgaccacg
      421 cccacctgca gttcggcggg tcgcacgacg acgcgtccga tcacgtcggc ggtcaacagc
      481 agcagcggac ccacgatcgc cgacagcgcc agcgcccgtc gcacgtcgcc gccgacgagc
      541 atccgcaccg catgcgggac gatcagaccg acgaagccga tggggccgga gacggcgacg
      601 gcggcgccgg agagcagcac gacggcgacg cctgtgagca ggcgggtccg taccacgtgc
      661 acgccgagtg cggcggcagc gtcgtctccg agagcgagcg cgtcgagagc tccggccacg
      721 acgagcgcga tgcccgcgcc gacgagtacg gcggcaccgg cggggatcag cacgttcgcg
      781 tgcgggatgc tcagcgcgcc gacgacccag aagcggtact gatccatcgt cgacgcgctg
      841 aacagcacga tgcccgagac gacgccaccc aggcaggcgg tgacggccat gcctgcgagg
      901 gtcatgttga cgggggtgag cccgtcggga gctcgatggg cgaacagata caccagtgcc
      961 gtcgcgaccg ctgcgcccgc tatggcgagc agcactcgcg gtaccccgcc cccgacaccg
     1021 aacatcaccg atccagcgac gatcgcaacc gccgcgcccg ccgagatgcc gagcagcccg
     1081 gggtccgcga gcgggttccg ggtcaccgcc tgcgccagca ctcccgccgc tcccagagcc
     1141 gcgccgacca cgaggccgac caccgtacga gggaggcgca gctgccagat gatcgcggac
     1201 gcgacatgat cggtccccgt cagcccgccg agcatctccc tgacgctcag cacgcgtgct
     1261 ccgatgagca ggctcagcac tgccgccagg agcagcagcg cggaggccgt gacgagggct
     1321 ccgatgagcg gcgcccgccg caccggtctc agcttcgtct cggtcactgc gggtgtcaac
     1381 gccgaccggc ggcgatggcc tccaggtcgt cgagcacgag ttgagcaccc tcgacgccga
     1441 ggcccaggta ccaggtctcg tcggacacct gcttgaccac gccatcgtcg gcgacggctt
     1501 tcacctgtcc ccagagcggg ccgtccacga cagcggcctg ctcggtcgca gcagcatcgc
     1561 cgtacgtgcc ccacagcagc cagtcgcctt cggcacgggt gatctcctcg gccgagatct
     1621 cgacggcgag ctcgtcgacg tcctcggtct cggtgcgcgg cagaccggcg tcggcgagga
     1681 tgatgccgat gaacgagtcg ttcgcgtaga gccggatgcg gccggccatg aagcggagca
     1741 tcgagatcgt cggagccttg cctccgttgg cctcgacgac cttctcgccg atctcggccg
     1801 cgcgctcctc ataggcatcc agcagcgact gcgccttcgc cttctcaccg accgctgcgg
     1861 cgaacagcag gaagtcctcc ttccagagca cgccgggccg cggtgagagc accgtggggg
     1921 cgatctcctg cagcttcttc ttcagcccgt cgtcgtcgat gcgcagcgag gagccgatga
     1981 tgaggtcggg ctcgagattc ttgatctgct cgaggtcgag tgcgtcatag ggcccgaggt
     2041 ccaccgcatc acccgcgtcg agatagtcgg gcgcaatcgc ggcctcggcg gggtgtgcga
     2101 tgccgatcgg cttcactccc agagcgagca tgttgtccag ctcgccggtg tccagcacga
     2161 tcacccgctc cggtcgctcg ggaatggtcg tgatgccgac cttgccgttc gcgacgatgt
     2221 tgcgcacgtc gcgcgggaac tcgccgtcct tcgcatccgt cccgagttgg gcggcgagct
     2281 tcctgacgtc ggagaagccc tcctgaccgg tcgcgacgtt ggacttgttc gctgccgcac
     2341 cgccaccgcc tgtcgattcc gcgtccgcag cgcaccctaa cagggcgacg gcgactgcga
     2401 ggatcgcgat gagtggagcg gcgagacgag gggagagcgg catgcggctt cgagtttcga
     2461 gtcggggatc gggaagggtg cggcgatcta gtaaggatcg ccttgcctaa gattacacga
     2521 gcgtagggcc cactccggat gccctacgct cggggtcgta ggcctgatcc atcccgagag
     2581 gagccgcatg gaaccccgtc gcttccctgc tggacatcca tcgggccggt catcgtgagc
     2641 cggcgctcca gcggcatcct cgccgtcgcc gtcacctccg tgctgtgggg caccaccggc
     2701 acagccgcca cgttcgctcc gtccgcgagt ccgctcgcga tcggatccgc cgcgctcggc
     2761 atcggcggac tgctgcagtt cgcggtcgcc tggcacgccg tgaccggcgg ccggacgcgg
     2821 ctcgcgcagc accgaggcat catcctcgcc gcgggtctcg ccgtggccgt gtatccgctc
     2881 gcgttctacg cgtcgatgca tctggcgggc gtcgcgatcg ggtcggtcgt gtcgctggca
     2941 tccgctcccc tgttctccgg cgccctggag gctgtcatcg accgccatcg tcccgacgga
     3001 cggtggacag ccgcggtcgt cctcgcggtc ggcggcggcg ccgcgctgat cctctcgcac
     3061 gaggtcggaa gcggctcgaa cgtcgttctc ggcgtcgcgc tcggcctggt ggccggtgcg
     3121 acgtacgcgc tgtactcctg ggccgcgagg cggctgatgg atgccggaat cgagcgcgcc
     3181 gcggcgatgg gcgcgatctt cgggctcggc gggctgctgc tgatgcccgt actgttcgtc
     3241 accggcgcgc cgctggtcgc atcgccgcag gccttcgcgg tctcggccta catggcactc
     3301 gtgccgatgt tcctcggcta cctgctgttc ggcttcgggc tggcacgtat cccggcgagc
     3361 accgcgacca cggtgacact gctggaaccg gccgtcgcgg cgctgctcgc cgtgctcgtc
     3421 gtcggcgagc ggctctcact cgtcggctgg gtcgggatgg ccgcgatcgc cgcctcgctg
     3481 ctggtgctga gtctccccgc gcgtgcgggt ggggccggag atcggtccgc gccgatgccg
     3541 tgaagcgggc atccgctgat cgggccgcga cggcccgttc cgatcagtac tccggggcca
     3601 gccccgcgcg caccgcggcg gcgaatcggg cgtatccctt ctcgttgaag tgcagccgat
     3661 ccgcgcggaa cagggagccg tcgatcgacc cgtcggcggt gagcagcgca ggcgcggtgt
     3721 cgatgaacac gaagtctccg ctgcgctccg cccgctccgc cagcatccga ttcgccgccc
     3781 tgatctcgtc gcgcactcgc gcccgggacg gcgcctcggt gatcgctacg tagtagatcc
     3841 tggtgtccgg cagctcggcc cgcacccggg cgatgtactc gtcgatgagc gagacggtct
     3901 cctgcgcgga cttcgtgaac agcgggatgc cgctgatgtc gttcgatccg atgtacagca
     3961 cgagcgcccg cgggtggaac ggcacgacca tgcgatcgaa gtgggcgagg tggtcggcga
     4021 ccttcgtccc gccgatcccg atgttcgtcg tgtggagagg ggcgagatcc gcggtcgaat
     4081 cctcccagta ctcgaagaac gagctgccgc tgagcagcac cccgccctgc ggatgtccgt
     4141 cggcgaaccg cttctcgacc gcatcgacct ggccctcgta ctgccgggga tcgtcgcggc
     4201 cgaaccacca cagcatgacg agaccgccga tcgcgcatag tgcagccgcg gcgccgatga
     4261 cccacgagac cgccacgacg gtcaggcgcc tcggacacct gcgatgtgat gaccgagcca
     4321 tggaactcct tcgaggtcgg gacgggctct cagagcccct cgagaatagt cgtcaccttc
     4381 tgttccaatg tgtgggacct actccagtgt gaggtgtgag acgtcgacgg tcatgcgggc
     4441 gccccttcgg agaccgcctc gagggcggac gcgaggtcgt tcctgagctc gtccgacgct
     4501 cccgggacgt attcgagcat cgtcgcgacg gtcatgaaag ccgcctcggg ggcggtcccg
     4561 tccagtggca ctcccgggaa gtgcgccgcg aacgcctcag tgaaggcgcg ccacgccgcc
     4621 ggcgcgtcgg cgagatcctt gagcgtcgaa tcgagggagt accgccgggg gccggcgccg
     4681 gcgggaagct cgtagctcca ggtgtgcacg ccgctcgcga tgtgatgcgt ctcgccatcc
     4741 ggatgcagcg gcaggatgac ctccgccgac gcgttctcag gaatgtcgac cgtcagcgag
     4801 acagtcccgc ccgagatctc ccaggcgatc tcgaccatgc cctgcaccgt cacgtgccgc
     4861 agacgcgccc aggtcaggcc gccgcccggg agcggcgcga tgcgcatccg ccggtaaccc
     4921 ggttcgatcg cggtgagacc tccgatgctg cgatgcatcc agtcgatgac ggcacccagc
     4981 gcgtagtggt tgagcgaggt catccccgag ggattgacgg ttccgtccgg gcggacggag
     5041 tcccatcgct cccagatcgt ggtggcgccc atcgtgacgg ggtagaggaa tgacgggcat
     5101 ccggtctcct gaagcagcag gtaggcctcg gcgaggtggc cagtgccgga gagggcgtcg
     5161 gcgacgagcg gggtgccggc gaagccggtg gagatccggt agccggcctt ggcaaccagc
     5221 tgggccagca ggtcgccggc atgcgctcgc tgtgcttcat cgaggatgcc gaaggcgagc
     5281 gcgagcgcgt agccggtgac cgtctcgcct gcgactcgtc ccatcggggt gacgtactcg
     5341 ctccggaacg catcccgcac ccgcttcgcg agcgccccga actcgaccgc atcttcctcg
     5401 tgcccgagga tctgcgcggt gtccgccatc tcctgcgtcg tcttgcacag gtacgccgaa
     5461 gcgacgaggg cgcggtcggt cttgcctccc gcggggttgt cgatcggggc gtcggggtcg
     5521 agccagtcgc cgtactggaa gccggtgctc cacaggccgt tctcgtcgag caggtctgcg
     5581 acctgacgga tgaaggtcgt catcgacggg taggcgtcgc gaaggatgtc ctcgtcgccg
     5641 tactcgcgat ggagaacaca cggcaggctg acggcgacgt cgctccacag cgcagtcggt
     5701 gaggacgggg tggagagcac atcgggaacc acccacggga cgaatccctt ttcccgctgc
     5761 tccgcggcca ggtcgcgcag ccaggagccg agcacgccgc ggacgtcgta gagaaaggcg
     5821 gctgcgggcg cgaacgcgtt caggtcgccg gtccatccca gccgctcgtc gcgctgcggg
     5881 cagtcggtcg gcacgccgac gaagttgtcg cgcatcgacc agacggcgtt ggagtgcagc
     5941 tggttcagca gcgggtcgga tgtctccagc cagccgatcc gttccatgtc cgagtggacg
     6001 acgaccgccc gcacggcgtc ggccgagaag tccccaggcc agccgtcgac ctcgacgtag
     6061 cggaatccgt ggaacgtgaa gcgcggctcc cacgtctccg ccgcgccacc gcgcaggatg
     6121 taccggtccg tcgccgcagc ggtgcgcagg gtctcggcct cgatctcgcc gtcgaccatc
     6181 acctcgacat ggcggagagt gatggtcgtt ccgcgctcac catcgacggt cagccgcacc
     6241 caaccggaga tgttctgccc gaagtcgagg acgagcctgc ccgagggcgt ggtgatcacc
     6301 tgctgcgggg cgagctcctc gatccgccgg atcggcggcg cgatgggctc ggcgaggctg
     6361 gcgagaggcc agtcgaagga gtcgacccgc acccactcag ccgcaccgaa acctgcgcga
     6421 tcccagccct cgagctcctc ccgggcatcg aagacctcgc cgtcgtagat gctgttggcg
     6481 cggactccgc ctcgggctgc gacgacgccg gtgtcgctga cgacgaactc ggtcgtgccg
     6541 ctctcgtact gcagctcgag ctcgaaccat gccgcggggc gatcggagta gtgccgtcgc
     6601 ttcccctccc acccgagccg gcccaccgcc catccctctc cgagcactgc gccgacgacg
     6661 ttcacaccga cggcgatgcg atcggtcacg tcgtgtgtgc tgacgatgag acggtgccga
     6721 tacgaagtcc acccgggagc cagcacctcg tcaccgacga cagccccgtt caggtggggc
     6781 tcgatgaggc ccaccgccgt ggtgcgcagc acagcccgag ccggccgggt cgcgacggtg
     6841 aactccttgc ggagatatga tgcgggctct tcatccgcgc cctgcgcgaa cggcgggctg
     6901 atgaagacgg cgcgagagtt ctcagtcatg gatgcgggca tcctttccgg gatggagtca
     6961 gcgaacagag cgaatgcgga gcacggaggc gccgccgagg accacggcga ccgcgccgac
     7021 gatgaagacg agcggatagt tccgggcgcc gcctgacacg ccgaccagga gcagcccgga
     7081 tgcgaccagc ggcatcaccg actgcgggat cgacgtcgcg aagccggtga tccccatgaa
     7141 cctgccggcg tccgtctcgc gttccggcag cacgtccagc agcagcgcct gatcgaccgc
     7201 ggcgaacagg ccgaggccca ccgatccgat cagcgatgcc acgatcagca gtgtgaggtc
     7261 cggggcgaac gcctgcatga ccatgcccgc cgccatgacc actccgccga gcaggacgaa
     7321 ggcgcgtcgc cggcgcagcc tgtcggacag gaagccgccg ccgatcgctc cggcggtcac
     7381 cgcgaggatc cccgccaggc tcaggcccgc catgatcccc gccacggact cgaccgtgat
     7441 cccgagccga tcggcgaaga agtacgcggt gtacgtcgtg ttgagcgtga tgcccgtgta
     7501 gaagaagaac cggccgagcc agttccacga gaagtcggcg tacctgcggg gtctgtagag
     7561 gtacttcgcc agcacctgca cgacggtgat cgggtccttg ggcagcccgc ggctgtcgtc
     7621 ctcgtgcaca agcgtcacga agaggagcac gaagagcacg cccaccgccc cggggaccag
     7681 gaacaacagc agggcgtccc cggtgaagaa ctgcgccagg atgacaccga agaccggcgc
     7741 gatctgggtc gcgaaactgg tgagtcccgc gaccttgccg cgctgcgatt ccggcagccg
     7801 gtcggcggtc gagatctgca ggttgctcag agtcgttccc cagccccact gcgcgaggat
     7861 ccaccccagc ccgacgacca ccgcgttcgg ggcgatcgcc atgaccacca gcgacaacac
     7921 tcccacgagc atcccgccga tcatgaacgg acgacgacgt ccgatgcggc tgcgggtgcg
     7981 atcgctccag atgcccatga acggcgagct cagcatcacg aacagggcac cggcgccggt
     8041 gatgaagccg aggtattcgg gatgcccggg ggcgagggtg ttcacgcgga tcgtcagcga
     8101 gatcgccagg ggcgtgatga aggccatgaa gacgccgaac tgcgccagca ccatgaacca
     8161 gatgtaacgg gcatcgacct tgggcaggtc cgggttggcc tgctcctcgc cgtggacgag
     8221 agcagctgga tcggctggtg gctggggcat gaccgccggg aggggcgcgg gacccggttc
     8281 ggcgtcggtg gggttgttca tcaagactcc atcgtcaggt acgggtgtcg gcgggacggg
     8341 cacgagcgag taggcgctca cccgcgatcg ggctactagc acgagctagt gcagtcacta
     8401 tagacgcgct aagctagcac gtgcaagtgc tagctggagt cgagacatcg gaggaagcct
     8461 gtggcgagtt cggaaaccct caagcgggtg acagcggccg atgtcgcgca cgctctgggc
     8521 atctcccgcg ccacggtcgg gttcgtcctc aacaacactc caggtcagaa gatctccgct
     8581 gtgacacggg agcgcgtgct ggcggaggcg aagcggctcg ggtaccggcc gcacacggcc
     8641 gcacgcgcgc tccgcagcgg tcagagccgc atcgtgctgc tgatccttcc ggactggccc
     8701 atggactaca gcatgcgcac ccacctggat gaggcttccc tcgtcctgga ccgggcgggg
     8761 tactcattgg tcacgatgac tccgcatccc ggcgggcagg cgcagccgct ctgggagacg
     8821 ctgcggcccg atgtcgtgct cggaatggca ccccttcccc gcgagatgac atccgagatc
     8881 cgcgaaagcg gggtgcagca tgtgctggcg gcagtgctcg aggaccagcc ggaacccgcg
     8941 cagggcgatc tcggtttcgt ggaaggaccc cggcttcagg tggagcacct actgtcgacg
     9001 ggtcgcagga tgctggcgtt cgccggctcg gcggacccgc gcatagccga cctggtgagg
     9061 atccggcgca ccctcgcggg gcggactctc acggaacgga ccggcaccgg gttcgcagcc
     9121 gacgcggaca tcgaccaggg caacgcggcc gaggtcctgg aggactggct gagccgagga
     9181 gtcgatggag tggtcgccta caacgacgac atcgctgccc tcgtcgccgg ctcggtgctt
     9241 cgctccgggc gctctgttcc gggcgacgtg gcggtggtcg gccacgacga cagcccgctg
     9301 gcatccctct tcgtccccgg tctgacgagc gtgcgcatcg acaccgcggg actcggacgt
     9361 tacgtcgcag aactggcgct gagcgccgtg accgattccc ccaagcccga ggccgggccc
     9421 gagaccgagg cgcatctcgt catccgccag tccaccggcg gatgacgagc cggttccggc
     9481 acctctggcc gcctgcgatt cacgccgtgc gtcggtccgc cgcaggatcg acgccagcgc
     9541 gccattggcg agatcgagcc aattcgcggg gagtacgtgt agacaaagca ggcagaagca
     9601 gagatccatc ccacgactct tacggcactg ggacgcatgc gaccccctgg gagcgagttg
     9661 cgctccacgt gttcagccgc gcgaccggct cagctgacgg aggcgagggc gctgagccct
     9721 cgcgcaaaga cgcgggggcc gcctccgagc atgttctcgt tctcggatgc cgcggccttc
     9781 ggcggctccg ctcgcgccgc gtcggaacgc cgaagaccta cagcatcgag cacgctcagg
     9841 cgcgcgcgtc gccctcgtca gtagtgatgc ggacgagcat cttgccggtg ttcgcgccgc
     9901 gcatcatctc gaggaacgcg tgcacggtgt tgtcgatgcc gtcgacgatc gtctcgtcgt
     9961 aggcgatgct cccctcggcg aaccacgccg tcatcttctc ctggaattcg ggagcaaggc
    10021 cgagatgtgc cgcgagcgtg aagcccttca gagtcagagc tcgggtgatg atgttggcca
    10081 tgttgtcggg gcctggggtg cggtctgtcg tgttgtaccc ggcgatcgct ccgcagagcg
    10141 cggcacgacc gccgtcgttg aacgcatcga gggccgcctc caggtggtcg cctccgacgt
    10201 tgtcgaagaa cacgtcgatg ccttccgggg ccgccgcggc gagctgagct cgcacgtcac
    10261 cgtcacggta gttgaaggcc gcgtcgtagc cgtacttctc ggtcaggagc gcgaccttct
    10321 cgggcgtacc cgcggaaccg atgacccgcc cggcaccgag cagcttggcg atctgtccga
    10381 ccgcggtgcc caccgcgcct gcggcgccgg agacgaacac ggtgtccccc ggcttcaacc
    10441 ccgcgatcgc ggtgagcccg acataggcgg tcaggccggt catcccgagg atgtgcagtc
    10501 gcagggacga agggaggccg ggcacgtcgg gcacggcgcg gaacgtcgac gcgtcagcct
    10561 ggaccacatc tgaccaaccg tgctgatgca ggacaagcgt cccgaccggg agttccgggg
    10621 cctcggagac gatgacacgg ccgatggcgc ctccggtgat cgtctcaccc agagcgaacg
    10681 gcgcgacata gctgcggacg tcgttcatgc gtccccgcat gtaggggtca acggagacga
    10741 actcgttgcg cacccgcact tcgccggcgg agggggcctc gtagaccacc tgcgtcgtct
    10801 ggaagtcgtc gagcgtcggc cagccgaccg ggcgcttcgc gagctggatc tgagtgctca
    10861 gggcagtgga catggaactc cttggtgatg gtgctgtcgg ccggggtcag agcgtgacgc
    10921 cgagcgcgag gatgatgacc gcgaccgcgg ggatgacgcc ctgcttcagc gccgcgcccc
    10981 gcttgtccgg cgaggagagc aggagcacga tcgcggccgc ggccatggag cccgccccga
    11041 cgaacacgag cgtcgcgccg accgcgtgcg cgccgacggc gaagaggacg attcccgtga
    11101 agaccgagat cgcgaggaac aggttgtaga acccctggtt gaaggccatg gacttcgtcg
    11161 ccagagcctc ctcctccgtg acgccgaacg tcgcacgcac tcgcggcgtg gtccatatca
    11221 gcgactccat gacgaagatg tagacgtgca cgagcgccgc gagaccggcg agtatcagac
    11281 ctgcgacaac catggggact cctccgtgaa gcgtttctgt aatgacctgt ccaatatata
    11341 ctggactgac cgttacacaa aaaggagcgc tcatggcacg cacaccgagc ttcgaccgcg
    11401 aagcggtggt ccgcgctgcc cgcacgctgt tctggacggt cggctacgag agcgcctcga
    11461 tccccgatct cgaggccgcc acaggcctca gccgctccag catctacaac gccttcggct
    11521 cgaagcgcgg cctgttcgac gccgccgtgc agagctacct cgacgaggtc gtccgcccgc
    11581 ggctgcgacc actgtccggc gaggtcgtcg atcccgaggc gatcatcgcg tacctgcagg
    11641 gcctgcgcga cgcattcggc acactcacct cgatgccggc atcgcacgga tgcctcctcg
    11701 tgaacgccgc cggcgccccg atcgcccacg accccaaggt cgcccaggtg atctccgact
    11761 atcgcgcgga actccacgac gccttcgccc gcggggtgga tgcgcgcacg cccgacctcg
    11821 acgccgccga gcgggaacga cgggtcaccg cgatcacgag cagcgtcgtc gccgcctttg
    11881 ccctggctcg gatcgacccg gccgaggccg ggcgaggcat cgcctcggcg atcgagctcg
    11941 tcgcccgggg cgcctgaacg cgccggaacg aaccgagccc cgagacttca tcccggggct
    12001 cgatttcttc ggcggagacg gagggattcg agtccctcgc ggccactcca cgcgccgccc
    12061 cgtctcagag gtcggtctcg tcgggcgcga agggcggcgc gcggagcctc cggccgcgtg
    12121 gggccctgtt gtcgaaagtg ccacccgtca cagcgaaaaa tccggacccg gggcttcgtc
    12181 ccaggatccg gattcttggc ggagacggag ggatttgaac cctcggtccc cttgcgagga
    12241 ctccacctta gcagtgcctt ttcgggcact tgcgccgtgt gatcctgcgg gcgaatgcag
    12301 atcatgcatg cggtatttcg caccgcacag acccctcact cccctgagac tcccccggga
    12361 caacgcgacg gtttcagcac ctactgggct tccaagacgg cgtgcgtgac tgggcgaaag
    12421 tggtcggaga gactccagct ccctgctctt cagcagtcca tccagtccca agtcaccgcc
    12481 atgatccgtt cggaagctgt ctagatatca cgttcgtcag gaggaagcga tccgaccatg
    12541 gaccacatca gcgcaggctg cccgtccggt gtcgattcgg acggagtcac gtgaaccatt
    12601 cgatcgtcgt cgagatggac cgtgacgacc tcattcgcga aactgcgtag tgcgtcgatc
    12661 gtcctcttga tatcgaggta gacgactgtg gtgcgagtca gaccggctgt gtcggcagga
    12721 agtcgaacgc tgccgatatt cgcgctctca tcgtcgtcgt tcattccgga gatgcgtagc
    12781 gttccgggtt cgaccgttgc gcgcacgcag ccgtcgcttg gccctcgtga tcctgccgtc
    12841 gccaagcgat gggcgtcccg caagaagcgt tgaaggtcgt tccgcgccac tgttgcactg
    12901 acgcgctggg ccgcgagctt ggggtggtag cggcgacgcc aggcgccgac tgcagtggtc
    12961 cacgcgctcc accggtcgcc ggcgatcatg agcagactcg tcccgtgctc ctgcgagatt
    13021 ccgatctgaa cgtgaccgtg agtcagagcg tctgacactt cacgcaacca agttgcgtcc
    13081 acgaaagcct cgaacgcatg ctcgtgggag accggcaggt tcgcctcggc cgcacggtat
    13141 cgatccgttg catcgatggt gagcacgcca tccacggcgc gcatgccgac tcgatcaagg
    13201 cggagtacgt catcggatcc tcgggaatcc gaagctgggc agacgtgaaa tattgctctg
    13261 cgcagggccg cggcgtcccc cacgacgact tcgtcctcag caaccggcgg catcgaaggg
    13321 gattcctcgc cgcgcctgtg gagcctagct cgatgagagc gaccgttcac cgagagacgc
    13381 aggtgcaagt tgtcttccgc atggagcgtg atcgatgcgg agcgacgaac ggccgccgtc
    13441 atccccttcg cgagagcatc agtgaccaag aacgcttcta caccatctac gagagcgatc
    13501 cccggaacgc tgacagtcgc cgtgatcgag ctgcaggcgg cagctcgatt gtccgcgccc
    13561 acgacccgca ccgagtccgc gtctgtcacg atccagactc ctcgttctgt ggtagccaca
    13621 cgccttgcaa gctccagcgc tcgataccac gcgacgaaag gcacatggac cgagaccgga
    13681 tcgctagcgc aaagctgatc ttccgagacc agggatcgat ctcgccaacc cggtaggtcg
    13741 acgtccgccc gcgcgagcgc ttcgaggacc ctcagatact ggtcgtccgt gaggccagtc
    13801 accagcctct tgaactcgtc tttgggaagc accgccacag atcacattct cgcggtcgac
    13861 agggtattca ggccagctct acccaccccg aggcgacggg acggtgagac tcaaggtgga
    13921 tcccgtcgaa tccgccacga acccgatcgg gcctgcattc gctcttgtgg acacccaatt
    13981 cccgtcatcg tcgccgatgc gcagctgtgg agttccgttc tggtcgtggt cgtcgagggc
    14041 ggttcatggg gacaaggccg acattagcga ttgcagcgac actactgatg actcccgacg
    14101 tcactgcacg cccgataggt ccaaggccac agacggcggt tgagcttggt tgatgggtgg
    14161 ggctccgatc gcggagtggt tcctgtgatg atgcgactca ggttcgccat ctagaccaag
    14221 catccgcgcg atcgtctcgc tggaactgtt cccggagtga ccttgtcccc cacgcgagtg
    14281 cacctctcgc cgcccctgca cggcgccgtt ggtacgtgta gtacgaaatt gctctgtcaa
    14341 aaccatctca tttttgctat gtttttgaca ccgaggtgtt ggggacatga ccgctcgcac
    14401 agaactaatg gccatcgcag ccgaacaaga cggctacgtc actctcgacg acgcgcgcgc
    14461 cctcgggatc gctgagcgtg atctgcaaca gctcgcccac cgcggcaaga tcatacgtga
    14521 agccactggc gtgtatcggt tcgaggagtt ccctgtgact cgtgccgcca gctaccgctt
    14581 tgccgtgctg tggaccggtc ggcctgaagc ggcgctctct cacgacacgg ctctgagtct
    14641 cctcgagctc tcggacatca acccgcccaa gacgcacgtg actgtgggaa cgggcgagcg
    14701 cattcgacga gcgggaggcg tggggatcgt cgtccacaag gaagaccttg cccccaagga
    14761 cctcagctgg tgggagggta ttcgctgcgt gaagccattc acggcgatcc ggcaggctat
    14821 cgacacgcat gtgccgttcc agttcgtcaa ccaggcgatc acggaggccc gagctcgagg
    14881 cagcatcacg gacgccgagc gagtcacgct ccgcagcaga ctcgaaaggg aacgatgagc
    14941 gatccattct ctgcgctgcc tgagaaaagc cgcacgccga ccagtgcatc gatcctgaac
    15001 caatgggtga gccatgcgga gcagatcgtc ggtgcacgcg gggaccgaac gcgctgggta
    15061 ctcgcctcga ccatcgtcgc agccgcgctg caacgcgcgt tgatggcgga cgacacacct
    15121 ctattcgtgg tgaagggcgg cgtgttcatc gagcgggcct tgaacctgca gtcacgcgcg
    15181 acgaaagacc tcgacacgct gttccgtggc gccgtggagg agttcgagca agccgcggac
    15241 gacgcccttg atgagccttg gggcatgttc gacctcactc gcaccgcgtt cacggtcatc
    15301 gagggcgcta agacaagtcg caagcctcgt cgattcaacg tattgctcga catcaagggc
    15361 aagcgctggg cgaacatcaa ggttgaggtc tcgttcccag aaggatcgat cgacaagcac
    15421 gtcggtacca tccctgctcc atccacgacg tttttcggca tcgagcaacc gtcggacctc
    15481 gcaaccatca cgatggccta ccaagtcgcc cagaagctcc acgcatgcac cgaccctcac
    15541 aatccaccgg acgagtccaa ccttcgggtg cgcgacctgg tcgaccttta cctaatcaag
    15601 cagacgttct acccggacga tgctgacctc acggaggtcc ggtcggccgc cgtcgacgtc
    15661 ttcagcgccc gtgccgaaga agccaaggcg gtcggcagcc cttcgcggac gtggccacct
    15721 gtcatcgtca gcaatgaatt gtgggagtct gagtgggcca agcctgcagg tgaagccggc
    15781 gtggtcctgt cgctggggga tgtgcttgag gccctcaaca aatgggtcgc cgccattgag
    15841 caggcggaag gcgcatcgac aggtagcgtc gacgagctca ccgaacagta gccgcattcc
    15901 cgattcgctc gtgtggcgca tccgaccgat ctgcatcaca atcactctgc cggcaatgct
    15961 cgatggacat cgcatcacgt gccagtggat ccctccgaga cgccggtgga gcgctgtggg
    16021 acccagcacc ttgcctgatc gcgaacgtgt cgacgatgtc ggaggtgcct agcagaatga
    16081 cggcgtggcc aagactcgct ccctgttcgt cccgtatgtg accgaccgcg tcatgcccga
    16141 agccgtcgaa tacctgccct tgggtcacga cgcgcggctc acagcggcga aggtctcggc
    16201 attcgttgaa gagaccgata gcctcccgga tgtgcggttg gctgttctgg gtgtgctccg
    16261 tgcgcacagt atgaacgggg cagagactcc gaccgcggtg atctccgtcg tcttcgctgc
    16321 gctcgctgtc ctcatcagcg tggtcgcaac gataggcggt ttcggcagcg ccgtcggcta
    16381 cgccttcggc gtgggactgg tggtactggg aggcgtgttc acgcgcgtcg cgttcaccgc
    16441 ccacgttcgc cggatgacgt gcggagtatg gctcgcagcc tacgaggacg ctcttaggcg
    16501 ctgacaccga acgctagggc gaaggcttcg tgcgcgtgat ccgcttcgcc gaggtagctg
    16561 agggcggttc cacgttcgcc tgcagacgga ccccgacgag ggcatgctgc cgcgacgtag
    16621 ggtccagccg caccggagtg acacggaacg taccggtgag atctcgacgg atcttccgcg
    16681 ggacgtagtc ggcgacgacg aggatggcga ctgggatgtt ggttgcggcg tactgaacgg
    16741 actgtgtgcc gaagtcatcc acgagttgct cgtcggtgcg atcttggtca attgttttca
    16801 cctcgatgta gatggtgcag cgtccgggga aggtgacggc gatatccacc cggccaccgg
    16861 caacgttcgg ccgttcgacg gccgcgtccc tgccggccat caacagggcc acgttcaagt
    16921 cgtccgcgag ttgatgttcc tccggccagg gctgcccctt ggccatccac ggcggcagcc
    16981 ggctcggtga ctgactgatg ttcaggtgat gggaggaata ccggatgagg tcgatcagca
    17041 acgtcgtcag ctcagtgcgg taggtgccga tgcctccctc ggcatcctcg tcgcattggg
    17101 cgagcaaggt ggtgaggagg tcattctcgg cgatgatcag ggagcccatc cggaacgcga
    17161 cctgctcgaa gacagggatg agggacggag ctgatgccgc cgcgatctcc agttcagaga
    17221 aggcgtcgtc cgtcaggcgg aggttctcgc gaatcacatc cggcagacgg atcgactcgc
    17281 caccggcttt ttttggtggg cgctcctgca gccgtgcaag gagactctcc accgcagcgc
    17341 gggtgtccga gccttcgggt tcggcgctta ccagcagcca ccgctgcatg tgcgtgatga
    17401 acgacgaatt cccctgcgcg atctcctcga cgcgcggcca gaccagctcc cgaacaccga
    17461 gctgtgatgc ctgttcggtc atgcccgggt tcaccacgag gaccatggtc tgctcggcgg
    17521 cgaacacgtg cgcgagcgcg cgcaccgtgg tctcggtgtc gaaccacccg tccgcgtcgg
    17581 ccacccggag ctgtgcaaga aggtccagcc acgcatccgt tgtgccggca cgggctcctc
    17641 gccagtccgg ctgcgtgccc cggtacccat cgaggtactc gtagacggcg ccggagatgg
    17701 cgtcgaaccg ctcctcggaa atggagtgtc ccgcgacctg ctcggtgacg ccggtgatga
    17761 cggctttgaa cgcggcggcg tccgggcgcg cgttgtcgtg gtaggccgtg tcgaaccagc
    17821 ggagtgcgtc gtcgagagtg ctgcgcacgg cctccacatc ggtggaggcc gccgcggatg
    17881 cgatcttccg gaaaccgagt tccatcgcgg catcggagtc gagatcgtcg tgcgtggtga
    17941 gcgcggtcag cgcaccgaga agctccgcgt ccccccacat cgagcaagcg gcgccgagcg
    18001 ctcgcactag cgggggcgtg gcgtccgccg cttcgcttcc ttcgtgcagc gaagctcgta
    18061 gctggagggt gttcttcgcc cagcgcccca gcgcaagccg cgtccatcct tccaacgcga
    18121 tgtctcgctc gaatccgcgc ccggcggcgc gggctctgaa cgctccttcc agacccggtg
    18181 cgatctgggg tagcgccgtc tccgcagcga gcagaacatc gtagagcgcg cgcacctcga
    18241 gcgccgggat ggcgcttgcc gcgctgatga gcagcgcgca cgccgacttc tgatcctgca
    18301 ggtcgcccca ctcggcgcac agcgcggtgg caacctccac gactcgggtg tcgccggcca
    18361 gcgcgtcaga tgaggcgtag atggtgtgct cgccgccgag attggcgacg gatggagcac
    18421 cctcgagcgt gtcgaggaag tcgaacaccg tctgtttcac agtggacctc tcctcaccac
    18481 atcagatctc cggcggtcgg ccgctgcacc gcctcgtcgg gaaggaccgc ggccaccaac
    18541 tcactgtgcg ccgtgctcgt aatgacctgg aggccgtgcg tctccgcaac ccggtccagc
    18601 tcccgcagca gcagctcgcc gttgtcgcgg ttgagctctt ccgtggcgag ggagtcaacg
    18661 atgagcagtc ccgggtgacg gcccattccg gaagccgttc cgacgtccag cagagcaacg
    18721 accagagcga tcttgagtcg tagcttttca ccgtcggtga gcttcccgaa cgccgtcttc
    18781 tcgccgccct tgatgacggg gagctgggcg ttcgcctgca gcgtcgcctt ctcgaggttg
    18841 ctgatgccca gcgtcttccc gagagtggtg atctgtgcgc ttaccgaggt gagcgcctct
    18901 gcgaagtcac tcttcgcgga cgctgcggcc aacacctcag cggcttcgag gatctgcgag
    18961 tcggtggtca acgcagcctc ctccggttcg acctctgatg gtcgggagcg ttcctgaatg
    19021 cggccatcga gtaccgcgat gcgacgttcg agcgcgcgcc tttcgatggg gtccccatgg
    19081 acgttacgca taacttccga cgcgcgttcg aactcgtcgt gcgcggcctt ggcctgtgcc
    19141 atagcctcgt cgtggagcgt cgagaaccgg tggacgtcag cggtgtgctg ggctacgcgc
    19201 tcttcggcca gcgcgagtgg gtcaaggttg tcgtcgttga cctcttcggc agcgtcgtcg
    19261 tcgtgaggag caccgtgagc ctcgtcgacg acgtcgtcaa tcgggttgtt gcagagcgaa
    19321 caggaccctt cctgctcacg ctgccactgc tccggctgaa ctttcgcatc gcaccgcggg
    19381 cagcacgacg gcttgagggc gtgccagaac cgcttggtga ccgcggcctc cttgaatgcg
    19441 acgaggtccc tctccgccgc atccaggtcc cgctccgcgt caccgtacgc gagagcggca
    19501 tccgacatcg cacgctgcgt ccgagtgatc tcggtcgtcg cggccgtcac ggccgccatc
    19561 gccgcctcaa cctcctcggc cgtcatcccg gataccggaa tggactcgag ttcggcggcc
    19621 gcttcagcgc gctcagctcg aagggctagc gtactcgcct cacgtgcttc ggtatcgcga
    19681 ttgcgtcgcc gagcgatgcc ggacagctgc gcgtcgacgc ctttcctgcg aacacgggcg
    19741 ctcagcgacg tcgcacccca gtcggtggag aggaacgtgt tgagcacgtt ccttgcctgg
    19801 aacggcgtct caccgatgac agaggactca ctcggtttgg taatagcgag cgcctgcgac
    19861 cataggtgcc agccatggtc gaccatattg ccgtcgtcgg cgtccgtcgc attcgccacc
    19921 cgcgtccact gcgtcagggc gtctagtccg aggcgctgca gcatgaaatc gtcgacaacc
    19981 aactccatct cgtcgtgccc atcgaaggtg tccacgagga gagcgccatc tgcgaggagc
    20041 cggtcacgca ggtcatcgac cggaacgttc agttcgaaca ccggctcacc ggccacttcc
    20101 gcctcttcgc tgagctctgt catcgcggcc gacgacagga agttgaagtc atcgaggtcc
    20161 gcacccacac ccgatgggag gatcaggatc tgcccgcgcg ggttcccgtc gtccacccgc
    20221 cacgcgatga gaacccgctc gccgccgatg gtgagcagca ggcacgcctg gcggagccac
    20281 ttctgcacat ccccggccac cctcgccgag ccacgcaggc cccacttcag gatgtcgagg
    20341 gtcgagctct ttcccgcgtc gttcttcccg tcgctgacga aggctgtcac cggcgtcgtg
    20401 aaggcgcggt agaaagagaa ggggacaaac tggcgagtct ctccatcgac gtcgtcggcg
    20461 aggttcttca ccccggcgaa gtggagcgcg tcgacgcgca gcgggcgacg cgtcggaacc
    20521 ggcttcggtc cgacgacccc gtagcgcgtg agcacctccg caacgacgtc acgatccgcc
    20581 tccacacgct ccgcgacggt gtccacccag tccactgcgg tcgcctccat cagccctcca
    20641 tgactttcaa cgcgacagct cgctcacgta cgcggtcgag aatcgagggg atgacatccc
    20701 cgtgtgcggt gccggcgtac tcggtctgca ggtactgtcg gcgcttggcg gacgcgccct
    20761 gagccgcatc ggcgatgagc ccgatggcgc tcgcctgctg cacgtaccag tccagctcgg
    20821 ggatctccgt gcgcatcgca gcgacggtat cgcggccctt ctgcaacagg aagtaatcac
    20881 gccgagacat cggtcccgaa tcggcagcgc gtcggtgctc gatctgtccg tagagcttga
    20941 gtaccgcgag agcatcgtcg atgaactcgt acgccccata gatgtagcgc tccatggggt
    21001 aggtgtgcag ctgcggcgcg tcaccttcca gcatgcgaac gacgtgcggc ttgatctggt
    21061 cgagcgtcac tcgcccgctc tcgaactccg tgagcaactc gtcggcgagg tagtcggggt
    21121 tgcggagcca gaagtcgagc ttctgaagtc ggatgagtga cgagacaaca gtgaccgcat
    21181 cctcgacgtc gcaggacgcg ggaaccgcca gacccagttc gtcgaggaga attagaaccc
    21241 gcgtcgcggt ctggctggtg gatccaccgc ggcggcgcgc gtcagatgag acggcctgca
    21301 ccggcgtccc cctcggcgcg aggttcggcc gctgcggcgc cgtgcacggg cttagagccc
    21361 agcatcgatc cataaaacgc gcgggccatg gctggtgagt tcgtgatcgg attcctcgtc
    21421 tcgacagtga atgccagcca tccgctccga gcccgtttgg gtagttggac gtgccgctat
    21481 tagatagtgc cacccgaggg gccattcgtg aggaccctcg ccgtcggcga cacggctcga
    21541 gcgggactgc cttcccttga tggcagttgc gttatcggtg atcgctacga ctgagcgcga
    21601 tacgacgaca ctctgtcact gcgcaggccc catagtggca gacattgccg agtggccacc
    21661 caccgcacgt gggtcatcct gtgagcagaa atcgttgtcg ccgaccctca tcgcacatca
    21721 ccgccgcgat tcgtccacac actccgcgcc gtgaatagcc cggccgccgc accccgaacg
    21781 aacgcagatt cgatgaccgc cccaggctgt tctagggaca ccacccctgc ggcgaggtgc
    21841 acgttgccgc gaaccatgga tcgaaaccca tgaatacgca gcgaatcacc tatccaacgc
    21901 gagcccgcac tcccccgtgg tcaccccggg ggagtctgtg cgaccagtcc tcacgtcaaa
    21961 tgaaggcttg gcggcgctgg acaaaaggga ggaaaacccg cactgaccag gaaccatgtg
    22021 gcggagacgg aggggtcacg tcgtccctcc acccgtcgct gcgcgccggg cggagcctct
    22081 gacgacgcgg ggccgaaatc cacgcgaggc tgcgcctcgc tggttcaaat cccactctgc
    22141 ccgggaaatg ttcaagcccc cagactccgt ccgggggctt gaacatttgg cggagacgga
    22201 gggatttgaa ccctcggtcc ccttgcgagg actccacctt agcagggtgg tgcactaggc
    22261 ctgactatgc gacgtctcca tgtgctcgaa ccgaatcgag cacgtcatgc aatcataacg
    22321 ggtcggacgg agtgcggcga actcgcctcc tgcatcgggc gtggcggtca gcgaccgcgc
    22381 acgttgccgt tcgagcaggt cacctgggcc gccgaggtgc ccgtgatcga cgagggcagc
    22441 tccacgacat ccgcgggggt cccagtcggc gtgggcgtgg ccggcggcgc ggtggtgccg
    22501 gcatccggct ccttcacgac cacaccgtcg ttcttcgtgt tctcgtgcgt gatctgcagc
    22561 tgcttgttgg ccgtgatcgc atcccacagg atcttggcgt tgcgcttgtc cgggatgact
    22621 cggtccgtgt cggccgggtc gtacaccgtc gggtactgca cgaagacgat gtcctcgaac
    22681 ggcacatcct tgaccgccag cgcgatctgc gcgatggtca tcgggttcgc gagagacgag
    22741 gacgcctcga ggttgttcag gcccacgtcg gcgagcttca gcatggtgcc gacgttgccg
    22801 agcacctgac cgctgaccag cttgcgcacg aggctcgaca tgtactgctg ctggttgccg
    22861 atgcgcccca ggtcgctgcc atcacccacg ccgtggcgcg tgcggaggaa ctgcagagcc
    22921 tgcaggccct ggatcgtgtg gtcgcccgcg gacatgtcca gaccggtgaa gcggtccttg
    22981 atcggattcg ccaggcagac atcgacgccg ccgatggcat ccgtgatctc gatcacgccg
    23041 ccgaaggtga ccttcgcggc gaactcgatg tcctcgtcgc cgaccaggct cgagatggtc
    23101 ttggcgacgc agtttaggcc gcccttgtcg ccgccgtgct cataggccac gttgagctgc
    23161 tgcttgctca tggccgaagt cgtcttgccg ttggcatccg tacaggacgg gatcgggatc
    23221 atcaggtcgc gcgggaagct caccacggtc acgcgccgcg gcgcggcgga gacgtgcacg
    23281 agcaggttca cgtcgttcag tgtgcccttc gagtccggcc cggtgcagcg tttctcgaac
    23341 aggtaggcgt acttcggctc gcaggtgtcc acacccgtga gcaggatgtc gaatccgccc
    23401 tggtacgccg agatgtccgg cgggagctcc ttctggttct ccagctccac ggcattggcg
    23461 ctgacggtgt tcagcaggcc acccacgacg aacgtgccca cgccgagccc gctgaccagg
    23521 atgacggcca gggcgatgcc gagcagcttg aacagctgcc cggtggcgcc gggcgtgcgc
    23581 agctgaccat gacgagcgac gggggtgcga cgacgggggg actcgctcac tcggtgcctt
    23641 tcgaacagat gcggagggtg tgggattcga acccacggga cattgctgcc cactggtttt
    23701 caagaccagg tccatcggcc gctcggacaa ccctcccgat cccggctcgc accggacgat
    23761 caggcgtcga gtctatcggt tccgggggag gagccgatgg tcgatgtctt cgattctccc
    23821 gtgtccacct ggggaactac tgggcggatg cctcagagca gcgtgcgaag cgcgatcgcg
    23881 agaccgccgt cgtcgaccac ggtggtctcc tcgccggcgg cgtccttcac ctcgtccggc
    23941 gcctgtccca tcgccaccgc gcggccctct ccggcgagcg cccaggcgaa cattcccagg
    24001 tcattgcgcc cgtcgccggc gaccagcacc cggtcgcggg ggatgccgag ctcggcgcgc
    24061 acgcgctcca gtgccgtgct cttgtcaacg ccctgcgggg cgatgtcgag ccacgccgtc
    24121 cagccgatcg cgtaggagac ctcgttgagt ccgacctcgg tgaccagctg atggaagtcg
    24181 tcctcgtcgt gctcgggcga gaccacgacg acacgggaca cgggctgcga gatgagctcg
    24241 tcgaagtcga cctcgcgtcc gccgtccagg gtccagtcgt ccagcaccgc cgtgtacagc
    24301 cgctggccgg actccagttc caccatgtac cgcgcctccg gcagccggtc gcgcagcagc
    24361 gcgagcacgg gcgcgggatc gaagacctcc acgtgccagc gcacccattc gtcgccgacc
    24421 cggcgcatga tcacggcacc gttcgagcac accgtgaact ccgcgctcag gccgagcacc
    24481 tcgacgtacc gccgggtgcc cgaccagctg cgcccggtgg cgagcatgac gtcgtagccg
    24541 gcatcccgca cttccgtgac ggcctcggtc accccggggc tcagcgtctc gtcctgcagc
    24601 aggatcgtgc cgtcgatgtc cagcccgacc aggccgcgcg agctcactcc gctgcctgcg
    24661 ccgcggggtc gacccagccg agcgggcgca gcacctcgat gccgcccagg tacgggcgca
    24721 gcacctcggg caccgtcacc gacccgtcgg cctgctggtg cgtctccagg atcgcgacga
    24781 tccagcgcgt ggtggccagc gtgccgttga gggtggcgac gttgcgcgtc ttcgcctggc
    24841 tcgcgtcgtc cgactcgggc cggtatcgca cgtcgagacg gcgcgcctgg tacgtcgtgc
    24901 agttcgaggt ggaggtcagc tcgcggaacg cgccctgcgt gggcacccag gcctcgatgt
    24961 cgaacttgcg cgcggcgctg gagcccaggt cgccggcggc gacgtcgatc acgcggtagg
    25021 ccaggccgag tgcggtgagc atctcctcct gcagcgcgac cagccggtcg tgctcggcct
    25081 cggcatcctc cggcgtcgtg tagacgaaca tctccagctt gttgaactgg tgcacgcgga
    25141 tgatgccgcg ggtgtccttg ccgtacgacc cggcctcgct gcggtagcag gtggaccagc
    25201 cggcgtagcg gtgggcgcct gaggacatgt cgaggatctc gtccttgtgg aagcccgcga
    25261 gcggcacctc gctggtgccg accaggtaca ggtcctcctt gtcgaggtgg tacacctcgt
    25321 cggcgtgctc gccgaggaat ccggtgccct gcatgatctc cggccgcacc agcgtcggga
    25381 cgatcatggg cgtgaagccg ttctgcagcg ccttgtcgag cgcgaggttc atcagcgcga
    25441 tctccaggcg cgcgccgatg cccttcagga agtagaagcg ggatccggag accttcacgc
    25501 cgcgctccat gtcgatggcg cccagcagct cgccgatctc gaggtggtcg cggggttcga
    25561 agtcgaaggc ggggacgtcg ccgacgcgac gcagctccac gaagtcctcc tcgccgccgg
    25621 ccggaacgcc gtcgatgatg acgttctcga tcttcgcgag agcggatgcc gcggcatccg
    25681 ccgcctcgtt cgaagcctgc tgcgcggcct tgacccgctc ggccaggtcc ttggcctgcg
    25741 cgacgagcgc ggccttctcc tccttgggcg ccttggcgac cgtcttgccg aaggcgttct
    25801 gctcggcgcg cagcgactcg aacgcgttca gcgcgtcacg acgcgatcgg tccgcctcga
    25861 tcgcggcgtc gacggtctcg ggcgcgttcc cgcgcgcgag ctgtgaacga cggacgagct
    25921 cgggctcgtc gcggaggagg gtgagatcga tcattgtgca agtctaccga gcggctccga
    25981 cgcccctgag cggtcggtgc ggcggcggat gcggtcccta cgcgcccgtg atcgtgttca
    26041 gccgtagagt tgcgacatgt cggcatccgg gaacgcgcgc acgcacgcgg cgctcgtcct
    26101 caaccccgtg aaggtcgacg ccaggcggct gcgctcgacg ctgacggccc tgtcgaagaa
    26161 gcacggctgg gcaccgccgc ggttctacga gaccagcatc gacgaccccg gccggatcat
    26221 cacccgcacg gctctcgacg agggggcggc cgcggtgctc gccgccggag gcgacggcac
    26281 ggtgcgctcc gtgtccgagg ccatggccgg caccggcgtg ccgttcgcga tcgccccggg
    26341 tggcacgggc aacctcttcg cacgcaatct cggtctgccg ctgctcgatc ccgagaaggt
    26401 catggaagcc gtgttcaccg gcttcacgca tccggtggac atcggctggg catccctcac
    26461 ccgcgagaac ggcaccgaga ccgagcacgg attcgtcgtg ctggcgggca tgggcctgga
    26521 cgccgacatg atcgccaaca cccgcccgga tctgaagaag accgtcggct ggatcgcgta
    26581 cctcgacggt gcggcgcgct ccctccccgg cgcgaagccg ttccgcgtcg tgtaccagct
    26641 cgacgacggc cgcctgcact ccacgaaggt gcacagcatg ctgttcgcca attgcggcgc
    26701 cttgcccgcg ggcatcgccc tgatgccgga tgcctcgctc gacgacggca cgatggacat
    26761 cgcggtcatc cagcccaccg gaccgctcgg atggctgggc atctggcgca aggtctggtg
    26821 ggacaactcg gtgctgcgcc gctcgcgtgc cgggcgccgc gtgctggagc gccgcgggcg
    26881 gaactcctcg atccgctacc tgcgcggacg gacggccgac accgccatgg tctcccccac
    26941 ctccatcgag ctggacggag acgagctggg caccgccgtg cggatgtcgt gcaggatcga
    27001 gcagggcggc ctcagcatcg tgctgccgaa ggggcacgac gtctcgaact tctgagccgg
    27061 ccgctcagcc gatgacggga acctcagcgt cgaggtggtc gccgctcagc ttcagggtga
    27121 aggctccgtc cccctcccgc tcctgcggca gctggatcac ggtgacgcga ggtgccatgt
    27181 ccatggtgca tacgcggtcg atcgtcgcga aggagaccga gatctgatcc ttcgtctgca
    27241 gcacctgctc cggcatcggc gggcaggacg acgagccgta cgtcatcagc aggatgccct
    27301 ggtcgtcgaa ccagcccgcg gagggctgtc ccatgcccgt gtcgttcggc gaagacggga
    27361 gcgcatcgag atcggcatcc gccttgagct cgccgtaggt gacgtccacc tcgacatcct
    27421 tcgtgacgtc gacgccggcg ggaacgggaa acgcgatcgc ccgaggcccg aagtccgcgg
    27481 tgcagggcgt cttgccgtca gcacgatcct tcagcgtgac ctgcagggtc tgtccatccg
    27541 ccttcacctc ggcctgcgac ggctggcagt ccttcgagga tgagccccag gtgacgatgc
    27601 cgatcgacgc tcccccggcg agccacgccg cctcgacgtc ctcgccactg gggtccttcg
    27661 actgcgaggg ggcggttccg tccgtcccac ccgtgctggt gggcggcgat gatgtcggtg
    27721 tcgtcgagca gccggcggcg aggaaggcca cggcagtgag ggcgagcatg gacggaagca
    27781 ggcggagtcg ggggaggctc atacctccca gtgtcgccac acgggcatcc gtctcacaac
    27841 cgcactactg cagtgcagag gtgagacggg cgagattgtc gagcaccgtg gaccgcagcg
    27901 gctgcttgtc ccactcctcc agcgtcagct cgcggctgag tgaacggtat tcgtcctcga
    27961 cgacccgcac ctcgtcgacg aactcctcgc cgcgtacgag catggacacc tccatgttca
    28021 gaccgaagga gcgcatgtcc atgttgctcg agccgatcac ggcgacctgg tcgtcgatcg
    28081 tgaggctctt ggtgtgcagg atgtacggct tcgggtacat ccagatccgg atgccggcgc
    28141 gcagcagcgc ctcgtagtag ctgcgctgcg cgtggtagac gacggcctgg tcgccctcct
    28201 cggagacgaa cagctccacc tgcacaccac ggtcgacggc tgccgtcacg gcgagcagca
    28261 gcgcctcgtc ggggacgaag taggggctca cgatcatgat ccgctccttc gccgcgtaca
    28321 gcagcgccag gaacagtcgc aggttgttct cgacctcgaa tccgggcccg gacggcacga
    28381 tctggcagcc gagctcaccg gcgccgtgct ccacgcgggt cagctcgccg gcatccggca
    28441 cccgatcggt ctcggcgtag tagtcggaca ggaacaccgc gttcaggctg gccacgaccg
    28501 gacccttcac ccgcaccatc aggtcgaccc agtgcagccc gcgcttgatg ttcttgggca
    28561 ggttgtagga ggagtcggtg acgttctgcg agcccaggaa gccgaccgag ccgtccacga
    28621 cgaggagctt gcggtggttg cgcaggtcgg ggcgctggaa gcgtccgcgc agcggctgaa
    28681 tggggagcat cagatgccag tcggcgccca tcgcgtcgag gcgcttcagc gtctcgcggt
    28741 acttcggctt ccagcggttg gcccagtagt ccagcagcac gcgcaccggg atgccgcgtg
    28801 ccgcgacctc ctccatggca cggaagaagt tgtccgtcga ggcgtcgctc tgcaggatgt
    28861 agaactccac gtgcacgtag tgctcggcct cccgcatggc gtcggccatg tcgtcgaggg
    28921 actgctggta gtcgctgatc aggtgcgcct cgttgcctcc ggacagcggc agggccccca
    28981 gatgctggtt catcgcgacc agcggcggga accactccgg cgcgttcgga cgcaatgagc
    29041 cgaagttcag gtggctggcg gcctcggcga tgtactcgtt gacctgctcc tgcttgcggc
    29101 gccgcgcgcg cggcagcttg gggttgccga tcagcaggaa cagcagcacc ccgacgaccg
    29161 ggatgaggaa gatcgccagc agccaggcca tggccgaggt cggacgcctg ttgcggggca
    29221 cgacgatgat cgacacgacg cgcacggtga tgtcgatgac gaggtagccg agcacggccc
    29281 accagggcca gttgttgatg tcgaacaccg agccccctcc gtcacgacat tgatcactct
    29341 ggatcagagt agccgcagac gaggacagaa gccgcggatg ccgcgttcag cggtgcgagg
    29401 tggtcaggat gccttgggcg ggagcccgcg cgcggcacgt tcctcggcct cgatccgggc
    29461 gtgcaccttg cgctcggtgc gatcgaatcg caggatgccg cggagcacga tcacgaacac
    29521 cgcgctcacc gcgagggtcg gcagaatggc ccaccccact gccatccaga tgtcgtccac
    29581 ccctccatcg tacgcggagt tcctgtcgat ctccccgggc cgcccgttct ccctgcacag
    29641 tccgtgatcg cggatcactt ctgcacggat gccgaggatc acgcggatcc cgcctcgacg
    29701 cacgtgggcg cgcgacgggc acactcaccg catgaccacg atcatccatg ccaccgaacc
    29761 cgccgagctg ctcggcatcg tcccggcgct ggccggtttc accccgcggc gcagcgtcgt
    29821 gctgctgccg ttccgcggtg cgcgcacgca cggcgcgctg cgggtcgacc tgcccgcacc
    29881 ggatgccgac cccgaggcgt tcgcggatgc cgccgtgcac gcgctcctgc aggtcagtgg
    29941 cgtcgacgcc gccgcactgg tggtgtacac ggacgaccac ccgcttccgg tccccgacgg
    30001 cgtgctcctg ccgcagctcg tcctcgccga agccctgggc gacgtgatcc tggaggccgg
    30061 cctgcacctg gtcgacgcgc tgtgcgtgac cccggacggg tggggcgact atcgggagga
    30121 cgaccccgcg atccagccgc tggagagcat ccccgtcggt cacgggatac ccggcgtcgg
    30181 cgacgtccgc ggcgaccagc tcgcgggcgc cgagctgccg cagagtgacc tcgccgaccg
    30241 cgaacgggtc ggtcgcgccc tgacagagct ggaggaactc ctggatcggc gaccgtcgac
    30301 atcaccggcg cgagacgcga acccgatcgc gctgatcacc gccatggaag tgctcgacga
    30361 cctgcccggg ttcgtcgagg agctcatcga gcaaccggcc gaagacgacc cgtactgctg
    30421 cgcggctctg ctgtggtgcc tggctcggcc cgcgctgcgg gacgcgatcc tggtgcagtg
    30481 ggcgacggat cgggagttcg gatgccgtgc gctggaggcc cagatggagt tctcccgaca
    30541 gggcggagac atcccccgca cgatcggaga ggtgttcctg ggccgcgggg cacggccgga
    30601 ccccgatcgc ctcgggtgtg cgctggagct ggtgcgcctg gcggccgcac gggcaccccg
    30661 ccagaccaag gcgggcgcct tgaccgccgc cgcctggctc gcctgggcgc tcggccgctc
    30721 atcgcatgcc ggcgtctacg tggatgaggc gctccggatc gaccccgagc acagcatggc
    30781 ctctctcatc tcgacgatgc tgtccgcagc cgtgctgccc gagtgggtgc tacgacgcgg
    30841 gtgaccggtc gcgcgtcagc gccggcagga gcccgtcgcc acgcccttcg tcgcgttctc
    30901 ggcctgcgcg atgaccggca gcagctcggc gttcgtcatc gcgtagccga tgttctgctt
    30961 gacctcgtcc ttggcgaaca ccatgcccgc gacctggccg tcctgcgtga gcagcgggcc
    31021 gccggagttg ccgggctcga cctgcgcctg cagcgcgtag atgctgcgct cgtcgctgct
    31081 gctgccgtcg atgctctgga tcgacgcggt cgacgaggag agcacgcccg cggggacgac
    31141 ccggaaagga cctccgaacg ggtaccccat cacggcccct ccggcacccg gcgccaaggc
    31201 gtcggagagc ccgagcggtt tcgccgtgac atccgccgcg accaccgcga tgtcgtcgac
    31261 cggatcgaag tagaccactc gaccgtcgcg cgccggctcc cccggcagtt ccacgaccgg
    31321 cgaggtcacg cccgcgacca catgcgcgtt cgtcatgatg cggtcgggcg cgatcacgaa
    31381 accggatccg gtggagatga tgctgcactg gctggcgaca cccgagatct tcgcgaccga
    31441 ggcctgcgcg gccttcagac ccggcgtcga caggtcgatg ccgttggggt ccggcggcgg
    31501 agccgcaggg ctgtcggggc cctggactgt cggcaggggc acgctgccga acacgagcgc
    31561 tcgca
//
LOCUS       DATMSX010000497         4743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_55115,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000497 DATMSX010000000
VERSION     DATMSX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55115"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VN041_15930"
     CDS             <1..1155
                     /locus_tag="VN041_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWU30569.1"
                     /translation="ALRRVARWASRVRVVSNRVDAPALVEEFAQTCREEIDYLHEAAN
                     AERFAENFADDPRVASPAVVWERTTRKVLTLEDVTAIKINDTDALRAAGIDPAEVAQV
                     FGDVMLDQVFTHSFVHADPHPGNVFVTPVEGDAGGHPWRLTFIDFGMMAEVPDSIRAG
                     LRTLVIAVAGRDSHGLVTAAQQIGVLLPSARTAELERALTVLFARFGGMGFNELRDVD
                     PRELTDFGVEFGETMRSLPVQFPERMLLLIRAVSLTSGLCSALDPAFNIWEPVEPYAA
                     RLLRDESGGLLKDVGAQTVSNLGSLWRLPARLDSTLDRLDAGSLTFDISSLENRLDRI
                     IRVAHRAVSAVLFGGLLIGGILLLAAAPPLGIVLMCVSVVPLLHAVFGRR"
     gene            1234..2541
                     /locus_tag="VN041_15935"
     CDS             1234..2541
                     /locus_tag="VN041_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30570.1"
                     /translation="MAKMTSRALPALALAIGAVLALTACGAGSRTDNENATKVSCSYK
                     APAEKTTVNVLAYNSSAIDPFTDTMVKSCTTDKVTLKHDPIDFGGQVTKTTATLAGDT
                     GTYDIIETYGFVIPGFATDKKLVPLNDLWSKYADQYGLDKISESMRAGMSYDGKLYAI
                     PMQAQMFVMAYRTDIFKELGLEVPKTFDDMIAAAEKIKAAGKMEYPIALPWLATSDIA
                     TSFQATMNSLGADYVTADGKVTLNTPEAKKALESLIALKPYMDPQVTTFDQPKVQQQL
                     FNGTAAMAVMFSGRMIDLTKDSNSKFAKDFGFAAAPKASADAKYSYNRLSIDGWSIPA
                     NAKLDHDMLFQMMASAVSEDASKASVPAAYPAREGMVTAENSPYGEAANESMSTAMPP
                     VVSPILADVSNAITPIIVEVLTGKTSVDDGMAKMQAAGEKIAG"
     gene            2611..3486
                     /locus_tag="VN041_15940"
     CDS             2611..3486
                     /locus_tag="VN041_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30571.1"
                     /translation="MKAREFWLLVLPSLIVMGGLLVLPLLRTIQWSFEEVHYGVPGSF
                     VGIDNFVEALTDVRFGRAVMFTVVVTLVTTAILLVLGYLVAIGVNRLTTSRPVVLGIM
                     LVSYVLPQLVGAVAYSWLFDDNFGGLVNRLIGVFSGTQVLWFTDQVPNAILVISNVVW
                     QMLPFAMLIILAGLQGVPVELREAAKIDGASPFQTHVSVIIPTIKGVLGFVTLITIMD
                     VLRMFDNLIPLSPQAQSIGNESIMMYVYRMAFADGAQKLGLGSAINVLTIILILVMLT
                     PFIRGIFKEAKAEQR"
     gene            3483..4379
                     /locus_tag="VN041_15945"
     CDS             3483..4379
                     /locus_tag="VN041_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30572.1"
                     /translation="MTLPDLDMSTRAVTTAGRPSNARRTGRGKPPVITGILLGVLCVV
                     MLSPFIWMTLSVTKPTDVAFANPPVVWDYEPTLRAFVDLWQTTYFADYLVNTLVVAVI
                     STVIALIIGIPAAYALSRFPSYVSALLLVLALIFRALPRFAVVLPMYDISRALGIYDT
                     TFALAIALVAINQPFTIWLLRNFFADIPRELDEAAMIDGCTRIGMLRRVMIPLMGPGI
                     LTAGIFVFLFAFQEYLTALVLTDTSSKTVPVFIATQLGQTLPMLQQAGAASMLLTIPV
                     IVIAFIAQKYLVAGLSDGAVKG"
     gene            4384..>4743
                     /locus_tag="VN041_15950"
     CDS             4384..>4743
                     /locus_tag="VN041_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU30573.1"
                     /translation="MHPLVLLPGMNCTADLWAGADLEGAITPALDVPDIEAQVDRLLD
                     ELPPRFVLVGLSLGAIVGMALAQRAPARVAGLCVMSTNAKAPTGAQRSAWQDWLDRLD
                     AGESPRALQHGILPALLS"
BASE COUNT          762 a         1617 c         1524 g          840 t
ORIGIN      
        1 gccctgcggc gggtcgctcg gtgggcgagc cgcgtgcgcg tggtctcgaa ccgtgtggac
       61 gcccccgcgc tcgtcgagga gttcgcgcag acctgccgcg aggagatcga ctacctgcac
      121 gaggccgcga acgccgagcg cttcgcggag aacttcgccg acgatccgcg ggtcgcctcc
      181 cccgcggtcg tctgggagcg caccacgcgc aaggtgctca ccctcgagga cgtcacggcc
      241 atcaagatca acgacaccga cgcactgcgc gcggccggca tcgacccggc cgaggtcgcg
      301 caggtgttcg gcgacgtgat gctcgaccag gtgttcacgc actccttcgt gcatgccgat
      361 ccgcacccgg gcaacgtgtt cgtgaccccg gtggagggcg acgcgggcgg gcatccctgg
      421 cgtctcacct tcatcgactt cgggatgatg gcggaggtgc cagacagcat ccgcgccggc
      481 ctgcgcacgc tggtgatcgc agtcgccggg cgcgacagcc acgggctcgt cacggccgcg
      541 cagcagatcg gcgtgctgct cccctcagcc cgcacggccg agctggagcg cgccctcacc
      601 gtgctgttcg cgcgcttcgg cggcatgggc ttcaacgagc tgcgcgatgt cgatccgcgc
      661 gagctgaccg acttcggcgt ggagttcggc gagaccatgc ggtcgctgcc ggtgcagttc
      721 ccggagcgga tgctgctgct gatccgcgcc gtctcgctga cctccgggct gtgcagcgcc
      781 ctagaccccg ccttcaacat ctgggagccg gtcgagccgt acgccgcgcg cctgctgcgc
      841 gacgagagcg ggggactgct caaggacgtc ggcgcgcaga ccgtgtccaa cctcgggagc
      901 ctgtggcgac tccccgcccg cctggactcg accctcgaca ggctggatgc cggcagtctc
      961 accttcgaca tctcgagcct ggagaaccgc ctcgaccgga tcatccgggt ggcccaccgc
     1021 gcggtgtcgg ccgtgctgtt cggcgggctg ctgatcggcg gcatcctgct gctggcggcc
     1081 gcgccgccgc tggggatcgt gctgatgtgc gtctcggtcg ttccgctgct gcatgcggtg
     1141 ttcgggcgac gctgaccgta tacgtattca gaatctgacg cgttccgaaa aataggcttt
     1201 cctaacctcg gtgcatacgt ataccatcag ggcatggcaa agatgactag ccgggctctc
     1261 ccggcgctcg cactggcgat cggtgctgtg ctcgctctca ccgcgtgcgg tgcgggatcg
     1321 cgcaccgaca acgagaacgc gacgaaggtg tcgtgctcgt acaaggctcc cgctgagaag
     1381 acgacggtca acgtcctcgc atacaactcc tcggcgatcg accccttcac cgacaccatg
     1441 gtcaagagct gcacgaccga caaggtgacg ctcaagcacg acccgatcga cttcggcggc
     1501 caggtgacca agaccaccgc gacgctcgcg ggcgacaccg gcacgtacga catcatcgag
     1561 acgtatggct tcgtcatccc tggcttcgcc accgacaaga agctcgtgcc gctgaacgac
     1621 ctgtggtcca agtacgccga tcaatacggc ctcgacaaga tcagcgagtc gatgcgggcc
     1681 ggcatgtcct acgacggaaa gctgtacgcg atcccgatgc aggcgcagat gttcgtcatg
     1741 gcgtatcgca ccgacatctt caaggagctc ggtctcgagg tgccgaagac cttcgacgac
     1801 atgatcgcgg cggccgagaa gatcaaggcc gcgggcaaga tggagtaccc gatcgcgctg
     1861 ccgtggctgg cgacgtccga catcgcgacc agcttccagg cgaccatgaa ctcgctcggc
     1921 gccgactacg tcaccgccga cggcaaggtc accctgaaca cgcccgaggc caagaaggcc
     1981 ctggagtctc tgatcgcgct caagccctac atggatccgc aggtgacgac gttcgaccag
     2041 ccgaaggtgc agcagcagct gttcaacggt acggcggcga tggcggtgat gttctcgggc
     2101 cggatgatcg acctgaccaa ggacagcaac tccaagttcg ccaaggactt cggcttcgcc
     2161 gcggccccga aggcgagcgc ggatgcgaag tactcctaca accgtctgtc gatcgacggc
     2221 tggtccattc cggccaatgc caagctcgat cacgacatgc tgttccagat gatggcctcg
     2281 gccgtcagcg aggacgcgtc gaaggcatcc gtgcctgcgg cctaccccgc ccgtgaaggc
     2341 atggtcaccg ccgagaactc tccgtacggc gaagcggcga acgagtccat gtcgaccgcg
     2401 atgccgccgg tggtctcccc catcctcgcc gatgtgagca atgcgatcac cccgatcatc
     2461 gtcgaggtgc tcacaggcaa gacctcggtg gatgacggga tggccaagat gcaggcggcc
     2521 ggcgagaaga tcgccggctg acagattccg aagcgatgag tgcatggccc gggctctcac
     2581 ccggaccatg cactcggaga agggcgctcc atgaaggcac gtgaattctg gctgctggtc
     2641 ctccccagcc tgatcgtgat gggcgggctg ctggtgctgc cgctgctgcg caccatccag
     2701 tggagtttcg aggaagtgca ctacggcgtt cccggctcct tcgtcggaat cgacaacttc
     2761 gtcgaggcgc tcaccgatgt gcggttcggg cgggcggtga tgttcaccgt cgtcgtcacc
     2821 ctggtgacga cggccatcct tctcgtgctg ggctatctcg tcgcgatcgg cgtcaaccgg
     2881 ctgacgacgt cccgtccagt cgtgctgggc atcatgctcg tctcctacgt gctgccgcag
     2941 ctggtcggtg cggtcgcata ctcgtggctg ttcgacgaca acttcggcgg tctcgtcaac
     3001 cgactgatcg gtgtcttctc cgggacgcag gtgctgtggt tcacggatca ggtgcccaac
     3061 gcgatcctgg tgatctcgaa tgtcgtgtgg cagatgctgc cctttgcgat gctcatcatc
     3121 ctcgccggcc tgcagggagt tcccgtcgag ctgcgcgagg ccgcgaagat cgacggcgca
     3181 tcccccttcc agacccacgt cagcgtgatc atcccgacca tcaagggcgt gctcgggttc
     3241 gtgacgctga tcacgatcat ggacgtgctg cggatgttcg acaatctgat cccgctgtcc
     3301 ccgcaggcgc agtcgatcgg caacgagtcg atcatgatgt acgtctaccg gatggcgttc
     3361 gccgacggtg cgcagaagct cgggctcggc agtgcgatca acgtgctcac catcatcctg
     3421 atcctggtga tgctgacccc cttcatccgg ggaatcttca aggaagcgaa ggcggaacag
     3481 cgatgacgct ccccgatctc gacatgtcca cgcgagctgt caccacggcg gggcggccct
     3541 ccaatgcgcg tcgcaccggt cggggcaagc cgccggtgat caccggcatc ctgctgggcg
     3601 tcctgtgcgt ggtgatgctc agcccgttca tctggatgac cctgtcggtc acgaagccga
     3661 ccgatgtggc tttcgcgaac ccgccggtgg tctgggacta cgagccgacg ctgcgagcgt
     3721 tcgtcgacct gtggcagacg acctatttcg cggactacct cgtgaacacg ctggtcgtcg
     3781 ccgtgatctc gactgtgatc gcgctgatca tcggcatccc ggccgcctac gcgctctcgc
     3841 gctttcccag ctacgtgtcc gcgctgctgc tcgtcctggc gctgatcttc cgcgcgctgc
     3901 cgcgcttcgc cgttgtgctg ccgatgtacg acatcagccg tgccctcggg atctacgaca
     3961 ccaccttcgc gctcgcgatc gccctcgtgg cgatcaacca gccgttcacc atctggctgc
     4021 tgcgcaactt cttcgccgac atcccgcgcg agctggacga ggcggcgatg atcgacggct
     4081 gcacccgcat cgggatgctg cgccgggtga tgatcccgct gatgggcccc ggcatcctga
     4141 ccgcgggcat cttcgtcttc ctgttcgcgt tccaggagta cctcaccgcc ctcgtgctga
     4201 ccgacacctc ttccaagacc gtgccggtgt tcatcgccac gcagctgggg cagaccctgc
     4261 ccatgctgca gcaggccggt gcggcgtcga tgctgctgac gatccccgtg atcgtgatcg
     4321 cgttcatcgc gcagaagtac ctggtcgcgg gcctcagcga cggcgccgtg aagggctgac
     4381 cccgtgcacc ccctggtgct gctcccgggc atgaactgca ccgccgacct gtgggcgggt
     4441 gcggatctcg agggggcgat cactcccgcc ctggacgtgc ccgacatcga agcccaggtc
     4501 gaccgcctgc tcgatgagct gccaccgagg ttcgtgctgg tcggcctctc gctgggggct
     4561 atcgtcggca tggccctcgc ccagcgagcg cccgcgcggg tggccggcct gtgcgtgatg
     4621 tcgaccaacg cgaaggctcc caccggcgcg cagcgcagcg cctggcagga ctggctcgat
     4681 cgactggatg ccggggagag cccgcgcgcg ctgcagcacg gcatcctgcc ggccctgctc
     4741 tcg
//
LOCUS       DATMSX010000498         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_74155,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000498 DATMSX010000000
VERSION     DATMSX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74155"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..2015
                     /locus_tag="VN041_15955"
     CDS             327..2015
                     /locus_tag="VN041_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30574.1"
                     /translation="MGHIDVNAIALTLPDGRPLLDDTTFRVGAGSTSALIGPNGAGKS
                     TLLRIIRGDLTPDGGAVVIDGSLGVMDQFVGHGDAGTTVHDLLVRVAPARIRAAAEAL
                     EAAENALIEDDTERTQMRYATAIADYADAGGYEHETVWDQCTIAALGVPFERARYRDL
                     DTLSGGEQKRLALEALLRGPDDVLLLDEPDNYLDVPGKRWLEDRLRETPKTVLLVSHD
                     RELLARTADRIITLEPGGAGATAWVHGGGFATYHRAREDRLDRLDELRRRWDEQHEKL
                     RTLVATLKIKASANDGFASRYQAAQTRLRRFEEAGPPEERPPAQDFDMRLRGARTGKR
                     AVVCTRLELTGLMKPFDAEIWFGDRVAVLGSNGSGKSHFLRLLARGGTDPDRTLGHLV
                     ATSEALEPVAHTGAATLGARVVPGLFAQTHAHPEFAGRTLLEILHRGDDRRAGMPRDA
                     ASSALDRYGLVRQAQQAFDQLSGGQQARFQVLLLELSGATLLLLDEPTDNLDLESAEA
                     LEQAIARFEGTVVAVTHDRWFARSFDRFLVFGQDGEVYESDAPVWDERRVVRAR"
     gene            2053..>2950
                     /locus_tag="VN041_15960"
     CDS             2053..>2950
                     /locus_tag="VN041_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWU30575.1"
                     /translation="MDIEIGRGKRARRAYTFDDIAVVPSRRTRNPEDVSTAWTIDAFS
                     FDIPVLGAPMDSVVSPRTAIMLGQLGGLGVLDLEGLWTRYDDPQPLLDEIAALPAERA
                     TSRMQQLYSEPIKPELIKQRLAEIREAGVTVAGSLTPQRTQELYETVVAAGVDLFVIR
                     GTTVSAEHVSSVAEPLNLKKFIYDLDVPVIVGGASTYTAALHLMRTGAAGVLVGFGGG
                     AASTTRVTLGIHAPMATAVSDVAAARRDYLDESGGRYVHVIADGGVGTSGDMVKALAM
                     GADAVMLGVALARATDAPGQGFH"
BASE COUNT          439 a         1063 c         1036 g          412 t
ORIGIN      
        1 ctggcggctg ctgcgcctcg ccggtgcagg cagcgtgtcc gccgaccagc ccatgtcggt
       61 gctgatggcg ttgatcgagg cgctgccaca tccggtcgcc gaggcgttgc tgaccgagct
      121 gctcgcgcgg ctgtccgagc cccgctgagc gcggcccggc ccccatggaa gaagacaaag
      181 gacggttctg gggtcggatg accggcataa ggctccttcc atggagacgg aaggtcaggg
      241 aacgggcggc ggcgcacgcc tgcgcgtcgg gaagctgata gcctggtcgg ctcgctgctc
      301 gacagggcgc gcggaaggag cccgctgtgg gccacatcga cgtcaacgcg atcgcgctga
      361 ccctccctga cggacgaccc ctgctcgacg acacgacgtt ccgcgtcggc gccggatcca
      421 ccagtgcgct gatcgggccg aacggagccg gcaagtccac cctgctgcgg atcatccgcg
      481 gtgacctcac ccccgacggc ggtgccgtcg tgatcgacgg atccctcggc gtcatggacc
      541 agttcgtcgg acacggcgat gccggcacga ccgtgcacga cctgctcgtg cgggtggcac
      601 cagctcgcat ccgcgcggcg gccgaggcgc tggaggccgc ggagaacgcc ctcatcgagg
      661 acgacaccga gcgcacccag atgcggtacg ccaccgcgat tgccgactac gccgacgccg
      721 gcggctacga gcacgagacc gtgtgggatc agtgcacgat cgccgccctg ggcgtcccgt
      781 tcgagcgcgc ccgctaccgc gacctcgaca cgctctccgg cggtgagcag aagcggctcg
      841 ccctcgaggc gctgctgcgc ggtcccgacg acgtgctgct gctggacgaa ccggacaact
      901 atctggacgt gccgggcaag cgctggctcg aagaccggct gcgcgagacg ccgaagaccg
      961 tgctgctcgt ctcgcacgac cgggagctgc tcgctcgcac cgccgaccgc atcatcaccc
     1021 tcgagcccgg gggagcaggt gccaccgcgt gggtgcacgg aggcggcttc gccacctatc
     1081 accgggccag ggaggacagg ctcgatcggc tcgacgagct gcgccggcgc tgggacgagc
     1141 agcacgagaa gctcaggacg ctggtcgcga cgctcaagat caaggcgtcc gccaacgacg
     1201 gcttcgcgtc gcgataccag gcggcgcaga ctcggctgcg aaggttcgag gaggcgggac
     1261 cgccggagga gcgtccgccc gcccaggact tcgacatgcg cctgcgcggg gcccgcacgg
     1321 gcaagcgcgc cgtcgtgtgc acccgcctgg agctgaccgg gctgatgaag ccgttcgatg
     1381 ccgagatctg gttcggcgat cgagtcgcgg tgctcggctc gaacggctcg ggaaagtcgc
     1441 acttcctgcg tcttctcgcc cgcggtggca ccgatcccga ccgcacgctg gggcatctgg
     1501 tggcgacatc cgaggccctg gaaccggtcg cccacaccgg cgcagccacg ctgggggcgc
     1561 gggtcgtgcc gggtctgttc gcgcagaccc acgcgcatcc ggagttcgcc ggccgcaccc
     1621 tgctggagat cctgcaccgc ggcgacgacc gtcgcgccgg catgccgcgc gacgcggcaa
     1681 gctcggcgct cgacaggtac ggactggtgc gtcaggccca gcaggcgttc gatcagctct
     1741 ccggcggaca gcaggcgcgc ttccaggtgc tgctgctcga gctgagcggt gcgaccctgc
     1801 tgctgctgga cgagcccacc gacaacctcg acctggagtc ggccgaggcg ctcgagcagg
     1861 ccatcgcccg cttcgagggg accgtcgtcg cggtcacgca cgaccgctgg ttcgccaggt
     1921 ccttcgaccg cttcctggtg ttcggccagg acggcgaggt ctacgagtcg gacgcgccgg
     1981 tgtgggacga gcgacgtgtg gtgcgggcgc ggtgagcgcg tcgacggcgc cgggtagtct
     2041 ggtggggtga gcatggacat cgagatcggc cgaggcaagc gcgcgcgtcg cgcgtacacg
     2101 ttcgacgaca tcgcggtcgt gccatcgcga cgaacccgca acccggagga cgtctccaca
     2161 gcctggacga tcgacgcgtt ctcgttcgac atcccggtgc tcggcgcgcc catggactcc
     2221 gtggtcagcc cgcgcaccgc gatcatgctc ggtcagctcg gtggcctcgg agtgctcgac
     2281 ctcgagggac tctggacgcg ctacgacgac ccgcagccgc tgctcgacga gatcgcggcg
     2341 ctccccgccg agcgcgccac gagcaggatg cagcagctgt actccgagcc gatcaagccg
     2401 gagctcatca agcagcgcct ggccgagatc cgcgaggcgg gcgtcaccgt cgccggctcc
     2461 ctcaccccgc agcgcacgca ggagctgtac gagaccgtcg tcgccgccgg cgtcgacctg
     2521 ttcgtgatcc gcggtaccac ggtgtccgcc gagcacgtct ccagcgtcgc cgagccgctg
     2581 aacctcaaga agttcatcta cgacctcgac gttcccgtca tcgtcggcgg cgcctcgacc
     2641 tacaccgcag ccctccacct gatgcgcacc ggcgccgcgg gcgtactggt cggcttcggc
     2701 gggggtgccg cgtccaccac gcgcgtcacc ctcggcatcc acgctccgat ggccaccgcc
     2761 gtctccgacg tcgcggccgc gcgccgcgac tacctcgacg agtcgggcgg acgctatgtg
     2821 cacgtgatcg ccgacggcgg cgtcggcacc tccggcgaca tggtcaaggc cctcgcgatg
     2881 ggtgcggacg ccgtgatgct gggcgtcgcg ctcgcgcgcg ccacggacgc gcccggccag
     2941 ggcttccact
//
LOCUS       DATMSX010000499         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_46023,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000499 DATMSX010000000
VERSION     DATMSX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46023"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VN041_15965"
     CDS             complement(<1..596)
                     /locus_tag="VN041_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30576.1"
                     /translation="MPRVKITEVAREAGVSLSTVSRVMNGNPTVDPVLAERVREVAAR
                     LGYTANPLARSLVLGRTQTVSVVIPDLANPTFQAILRGLSHAAAKDGYHVLISDTEEQ
                     VHEEREIATTTRRRTDGVILCAPRMPQQELSALLPGIAPAVVINRPPLAEAPTVSADY
                     GVAITELIDHLRGLGHTRIVFLSGAVASASGEARLRAIT"
     gene            687..2558
                     /locus_tag="VN041_15970"
     CDS             687..2558
                     /locus_tag="VN041_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30577.1"
                     /translation="MPQKALSDPAALIDGQHPTRSVIRLIARRPWRLAVAVLAFAVKE
                     IPVWFLPVITAAIIDIVASHSDVTGVLWWFALAALLLVQNYPNHLIYTRSFMTVVRDL
                     GADLRNALTARLQSLSIGYHTRVSASIVQTKLVRDVENVELMLQQVTHPLLSSTMVML
                     GALTMTAIAVPQFLPVYALAVPIAIGIRYGMRKRSQLRNEEFRREIETLSARVGEMAS
                     LIPVTRAHGLEQTAITRVADGADGVRRAGLDLDLLNGRVASISWVAMQLLGVACLMLA
                     AVFAITGILPISPGEVVMLSTYFTLLTGGLTQLLMLIPVGARGLESVRSISEVLQEPD
                     VEQNSGRRIVGRVDGEIVLDAATHRYPDADDDALHAIDLRIAPGDTVAFVGSSGSGKS
                     TLLNLVLGFVRPSSGRILIDGQDMQELDVRTVRHSISVVPQESVLFEGSIFDNVTYGL
                     PEATPERVESALRDANAWEFVREQPHGWDTVVGERGARLSGGQRQRLAIARALVRDPR
                     ILLLDEATSALDPESEALVKEALERLMRGRTTLIVAHRLSTVRQADRIVVLEHGRIVE
                     QGSHDELMALDGRYVQLHRAQNGRPPVITGAIPLPTASEAARDPEPQGMIVPREARS"
     gene            2555..3451
                     /locus_tag="VN041_15975"
     CDS             2555..3451
                     /locus_tag="VN041_15975"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HWU30578.1"
                     /translation="MTLALTRTAAALSVTDGETELMRYVFVPDTPQLEAPKPYIDPIR
                     TRAGRVVSLFRPWDHVWHKGIAWSLPVVGDENFWGGPTYLRDQGYVQQPNDGTQRHVG
                     VVVAVVEHDVATFSHDLEWISERGQRMFTERRSLTARLLDDTAWALTFETSMTNATDG
                     TIAIGSPTTRGRENAGYGGLFWRGPRSFTDGLLVTAQGTGTGSALRGQHHEWMGYEGR
                     HDEVDAASLVVMLDAASNPHHPPQWFARSEEFAALNPAPFFSEEVWVPAGEAVSFRYG
                     VGVADADAGDARRIADRLRTLL"
     gene            3519..>3840
                     /locus_tag="VN041_15980"
     CDS             3519..>3840
                     /locus_tag="VN041_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU30579.1"
                     /translation="MPSHIPPAGALRCVLVGTGAVANLHAKAVAAHPRAELVAVADIS
                     ADRAAGFAEQYGIPASYGDYDDMLAAEHPDVVLICTPPAPHRAQALAAFAAGAHVIVE
                     KPPAP"
BASE COUNT          553 a         1390 c         1301 g          596 t
ORIGIN      
        1 gtgatggcgc gcaggcgcgc ctcgcccgag gcgctggcga ccgcgccgga gaggaacacg
       61 atccgcgtgt gtccgagacc gcgcaggtgg tcgatcagtt cggtgatcgc gacgccgtag
      121 tcggcggaca cggtcggtgc ctcggccagt gggggacggt tgatcacgac cgccggggcg
      181 atgccgggca gcagggcgga cagctcctgc tgcggcatgc gcggcgcgca gaggatcaca
      241 ccgtcggtgc gcctgcgggt ggtcgtcgcg atctcccgct cttcgtgcac ctgctcctcg
      301 gtgtccgaga tcagcacgtg gtagccgtcc ttcgccgccg cgtggctgag gccgcgcagg
      361 atcgcctgga acgtcgggtt ggcgagatcg gggatcacga ccgacaccgt ctgggtgcgg
      421 ccgagcacca ggctgcgggc gagcgggttc gccgtgtagc cgaggcgtgc cgccacctcg
      481 cgcacgcgct cggcgaggac ggggtccacg gtcgggttgc cgttcatcac gcgggagacg
      541 gtcgacagcg agacgcccgc ctcgcgggcg acctcggtga tcttcacccg tggcatccgt
      601 cctcctgtcg tcgggtcgag cctagctttc ggcgccgtcg tccccggatc ttgtccccgc
      661 atcgtgctcg gtcctacgat ggaaccatgc ctcagaaagc gctttccgac ccggctgcgc
      721 tcatcgacgg acagcatccg acccgttctg tcatccgctt gatcgcacgg cgtccctggc
      781 gcctggccgt cgccgtgctg gcgttcgccg tcaaggagat cccggtgtgg ttcctgccgg
      841 tcatcaccgc ggcgatcatc gacatcgtcg cctcgcacag cgacgtcacg ggcgtgctct
      901 ggtggttcgc gctggccgcc ctgctgctcg tgcagaacta tccgaatcac ctcatctaca
      961 cgcgcagctt catgaccgtc gtgcgcgacc tcggcgccga cctgcgcaac gccctcacgg
     1021 cgcggctgca gagcctctcc atcggctacc acacgcgcgt gagcgcctcg atcgtgcaga
     1081 ccaagctggt ccgagacgtc gagaacgtcg agctcatgct gcagcaggtc acccacccgc
     1141 tgctctcctc gacgatggtc atgctcggcg cgctcaccat gaccgccatc gccgtaccgc
     1201 agttcctgcc ggtgtacgcg ctggcggtgc ccatcgcgat aggcatccgc tacggcatgc
     1261 gcaagcgctc gcagctgcgc aacgaggagt tccgccgtga gatcgagacc ctgtccgcgc
     1321 gcgtcggcga gatggcatcg ctcatccccg tcacccgcgc gcacggcctc gagcagacgg
     1381 ccatcacccg ggtcgccgac ggcgccgacg gcgtacgccg cgcaggtctg gatctggatc
     1441 tgctcaacgg cagggtcgcc tcgatctcgt gggtcgcgat gcagctgctc ggagtcgcct
     1501 gcctcatgct cgccgcggtg ttcgcgatca ccggcatcct gccgatctcg cccggcgagg
     1561 tcgtgatgct ctcgacctac ttcacgctgc tcacgggcgg cctcactcag ctgctcatgc
     1621 tcattcccgt cggcgctcgc ggcctggagt ccgtgcgctc gatctccgaa gtgctgcagg
     1681 agcccgacgt cgagcagaac tccggccggc ggatcgtcgg ccgcgtcgac ggcgagatcg
     1741 tgctggatgc cgccacgcac cgctatccgg atgccgacga cgacgcgctg cacgcgatcg
     1801 acctgcgcat cgcacccggc gacacggtcg ccttcgtcgg ctcatcgggt tcgggcaagt
     1861 cgaccctgct caacctcgtg ctcggcttcg tgcgacccag cagcgggcgc atcctcatcg
     1921 acggtcagga catgcaggag ctcgacgtgc gcaccgtgcg tcactcgatc tcggtcgtgc
     1981 cgcaggagtc ggtgctgttc gagggctcga tcttcgacaa cgtgacctac gggcttccgg
     2041 aagcgacgcc ggagcgggtc gagagcgcgc tgcgggatgc caacgcgtgg gagttcgtgc
     2101 gagagcagcc gcacgggtgg gacaccgtgg tcggcgagcg cggcgcccgg ctctccgggg
     2161 gacagcgcca gcgcctcgcg atcgcccgcg cgctggtgcg cgacccgcgc atcctgctgc
     2221 tcgatgaggc gacgagcgcc ctcgatcccg agtccgaggc gctcgtgaag gaggcgctcg
     2281 aacggctgat gcgcgggcgg accaccctga tcgtcgccca ccgactctcc accgtccgcc
     2341 aggcggaccg catcgtggtc ctggagcacg ggcgcatcgt cgagcagggg tcgcatgacg
     2401 agctgatggc gctcgacggc cgctacgtgc agctgcaccg cgcccagaac ggcaggccgc
     2461 cggtgatcac gggcgcgatc ccgctgccga cggcatcgga agccgcgcgc gacccggagc
     2521 cacagggcat gatcgtcccc agggaggccc gctcatgaca ctcgcgctca cgcgcaccgc
     2581 cgccgcgctc agcgtgaccg acggcgagac cgaactgatg cgctacgtct tcgtgccgga
     2641 cacgccccag ctcgaggccc ccaagcccta catcgacccg atccgcaccc gggccggacg
     2701 agtggtgagc ctgttccgtc cctgggacca cgtctggcac aagggcatcg cctggtccct
     2761 gccggtggtc ggcgacgaga acttctgggg tgggcccacg tacctgcgtg atcagggtta
     2821 cgtgcagcag ccgaacgatg gtacgcagcg gcacgtcggc gtcgtcgtcg cggtcgtcga
     2881 acacgacgtg gcgaccttct cgcatgatct ggagtggatc tccgagcgtg ggcagcgcat
     2941 gttcaccgag cgccgatcgc tcacggcccg gctgctcgac gacacggcct gggcgctcac
     3001 gttcgagacc tcgatgacga acgccaccga cggaaccatc gcgatcggtt cgcccacgac
     3061 tcgcgggcgc gagaacgccg gatacggcgg cctgttctgg cgcggaccgc gttccttcac
     3121 ggacggcctc ctcgtcacgg cccaggggac ggggacgggc agcgcgctgc gcgggcagca
     3181 tcacgagtgg atgggctacg agggccggca cgacgaggtc gatgccgcgt ccctcgtggt
     3241 catgctcgat gcggcatcca acccgcatca cccgccgcag tggttcgccc ggtccgagga
     3301 gttcgccgca ctcaaccccg cacccttctt cagcgaggag gtgtgggtgc cggcagggga
     3361 ggccgtgtcg ttccggtatg gcgtcggcgt cgcggacgcc gacgccggcg acgcccgtcg
     3421 catcgccgat cggctgcgga cgctgctgta gccggccgtc cacgcgtccg gccggacgta
     3481 cttgaaacgt tatgatcaac ccaatccgag gaggatcgat gccatcccac atcccgcccg
     3541 ccggcgcact gcgctgcgtg ctcgtcggca ccggcgccgt cgccaacctg cacgcgaagg
     3601 ccgtcgccgc gcacccgcgc gccgagctcg tcgcggtcgc cgacatcagc gccgatcggg
     3661 ccgccggctt cgccgagcag tacgggatcc cggcgtccta cggtgactac gacgacatgc
     3721 tcgcggccga gcatccggac gtcgtcctga tctgcacccc gcccgcaccg caccgtgcgc
     3781 aggcgctcgc cgcgttcgcc gcaggcgcgc acgtgatcgt cgagaagccg ccggcaccag
//
LOCUS       DATMSX010000500         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149353,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000500 DATMSX010000000
VERSION     DATMSX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149353"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VN041_15985"
     CDS             <1..442
                     /locus_tag="VN041_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU30580.1"
                     /translation="VAAQQGLSARDRIEVPHADRTLAVPGLGVCVPERLPEGWLIRPV
                     ADTSVVAVLDLTGTQACIEVRADESAWRVPLTPRHADMLALLSDAGPEGVTAADLSRR
                     LHGDVDHQVTVRAEFSRLRRSAGALVAGAPYRIADGVLLSVRRA"
     gene            complement(447..1133)
                     /gene="lexA"
                     /locus_tag="VN041_15990"
     CDS             complement(447..1133)
                     /gene="lexA"
                     /locus_tag="VN041_15990"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWU30581.1"
                     /translation="MSENATPASEAPRTRRRKSLSAKQIAILEVIQTSIARYGYPPSM
                     REIGDAVGLKSLSSVTHQLGQLELSGYLRRDPGKTRAMEVLIDLPGTSTENPADSAPA
                     VGDAALVPLVGQIAAGIPITAEQQVEEIFPLPRQLVGKGELFMLKVNGESMIDAAICD
                     GDWVVVRTQSNAENGEIVAAMIDGEATVKTFRRRDGHTWLLPRNSAFEPILGDEAVVL
                     GKVVAVLRAV"
     gene            complement(1174..1437)
                     /locus_tag="VN041_15995"
     CDS             complement(1174..1437)
                     /locus_tag="VN041_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30582.1"
                     /translation="MRLLFVFPSIGGTGTAFASGIRPGTPDAKLCSESIFDIEHLSRI
                     LRNPSENVADTLEQIFRFPRILGYGFDMDSTPLATDIRTGSPR"
     gene            1438..1836
                     /locus_tag="VN041_16000"
     CDS             1438..1836
                     /locus_tag="VN041_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWU30583.1"
                     /translation="MSTISIQSPAILRASAGAASGASTRAVRSTKLRLTVRGRRVLAA
                     VAAAPLVAGIAFSVLAGGSALASGEQNAGVSFETVTVLPGDTLWSIASEVAPGVDPRD
                     VIDDIMSLNNLSSGAIQAGSEIAIPTQYSH"
     gene            1875..2966
                     /locus_tag="VN041_16005"
     CDS             1875..2966
                     /locus_tag="VN041_16005"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWU30584.1"
                     /translation="MGRVTSLDELPLRDDLRGLTPYGAPQAPLPVALNVNENTHPVPE
                     SVVGDILDDIALALSDVNRYPDREFTQLREALAAYLGHDLTADQIWAGNGSNEVLQHI
                     MQAFAGPGRTAFGFAPTYSMYPLITQGTGARWISGTREGDYTISPEDAAAQVAAADPD
                     VVLLCSPNNPTGTPLGLDVVEAVYEAARGIVVVDEAYHEFAPKDAASALSLLPGRPRL
                     AISRTMSKAFAFAGARVGYLAADPAFIDALRLVRLPYHLSALTQAAATAALRNSVTML
                     RMVDEIVEQRDRISATLEALGYAPHPSWSNFVLFGGIDDPKAVWQALYDRGVLIRDVG
                     IPGHLRVTAGTEAETTAFLDALASIGSPA"
     gene            2963..3568
                     /gene="hisB"
                     /locus_tag="VN041_16010"
     CDS             2963..3568
                     /gene="hisB"
                     /locus_tag="VN041_16010"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWU30585.1"
                     /translation="MTAPRTARRTRSTSESTVEVELNLDGTGRSDISTSVPFFDHMLT
                     AFAKHSLTDLTVRATGDTHIDAHHTVEDVSIVLGQAIREALGDKSGISRYGDALVPLD
                     EALAQAVVDISGRPFLVHEGEPAGFEMHLIGGHFTGSLVRHVFEAITFNGGLTVHVRV
                     VGGRDPHHIAEAEFKAFARAFRQAKALDPLVDGVPSTKGAL"
     gene            3565..4203
                     /gene="hisH"
                     /locus_tag="VN041_16015"
     CDS             3565..4203
                     /gene="hisH"
                     /locus_tag="VN041_16015"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWU30586.1"
                     /translation="MSTGPRVVVFDYESGNVHSAVKALAAAGADVELTGDRDRALEAD
                     GLFVPGVGAFSAVVDALRAHGGDEIIDRRLAGGRPVLGVCVGMQVMFERGVERGLNTE
                     GLGEWPGTVAELDAPVLPHMGWNTVEAGEGSVLFRGLEQERFYFVHSFAAKTWGIQVE
                     PPFPQPALTWATHGERFLAAVENGPLSATQFHPEKSGEAGIRMLRNWVESLG"
     gene            4302..5048
                     /gene="priA"
                     /locus_tag="VN041_16020"
     CDS             4302..5048
                     /gene="priA"
                     /locus_tag="VN041_16020"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HWU30587.1"
                     /translation="MNDFAQSPSLILLPAVDVAGGKAVRLTQGEAGTETSYGDPIDAA
                     GEWVDQGAQWIHLVDLDAAFGRGSNAPILRKVIKQYRGVNIELSGGIRDDASLEAALE
                     SGANRINLGTAALENPEWAADVISRYGEAVAVGLDVRGTTLAARGWTKDGGDIWEVLE
                     RLEEAGCSRYVVTDVTKDGTLRGPNLELLREITSRTPKPVIASGGVSNLDDIAALREL
                     VPLGVEGAIVGKALYAGQFTLAEALDVAGF"
     gene            5032..>5163
                     /locus_tag="VN041_16025"
     CDS             5032..>5163
                     /locus_tag="VN041_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HWU30588.1"
                     /translation="MSPASDGACDHGDTEGNTADSAGVPWAGRSFEPNTRAADDGSAD
                     "
BASE COUNT          762 a         1812 c         1792 g          797 t
ORIGIN      
        1 ggtcgccgcg cagcaggggc tgtccgcccg ggaccggatc gaggttccgc acgcggatcg
       61 caccctcgcc gtgccggggc tcggcgtgtg cgtgcccgag cgcctgccgg agggctggct
      121 gatcagaccc gtggcggaca cttcggtggt ggcggtgctc gacctcacgg gcacgcaggc
      181 gtgcatcgag gtgcgggccg atgaatccgc gtggcgcgtg ccgctcaccc cgcggcacgc
      241 cgacatgctc gctctgctct cggatgccgg tcccgagggc gtcacagccg ccgacctcag
      301 ccgtcgactg cacggcgacg tcgatcatca ggtgaccgtg cgcgcggagt tctcccgcct
      361 gcgacgctct gcgggcgcac tggtcgccgg cgcgccgtac cggatcgcgg acggagtcct
      421 gctgagcgtg cggcgggcct agccggtcag acggcgcgca gcaccgccac gaccttgccg
      481 agcacgacgg cctcgtcgcc gagtatcggc tcgaacgccg agttgcgggg gagcagccag
      541 gtgtggccgt cacgtcggcg gaaggtcttg accgtcgcct cgccgtcgat catggccgcg
      601 acgatctcgc cgttctcggc gttgctctgc gtgcgcacga ccacccagtc gccgtcgcag
      661 atcgcggcgt cgatcatgga ctcgccgttg accttgagca tgaacagctc gcccttgccg
      721 acgagttggc gggggagcgg gaagatctcc tcgacctgct gctccgcggt gatcgggatg
      781 ccggccgcga tctggccgac cagggggacc agggccgcgt ctccgacggc gggagcggag
      841 tcggccggat tctccgtgct cgtgccgggg aggtcgatca gcacctccat ggcacgcgtc
      901 ttgcccggat cgcggcggag gtagccgctg agctcgagct ggcccagttg gtgggtgacg
      961 ctggagaggg acttgagtcc gacggcgtcc ccgatctcgc gcatgctcgg cggatagccg
     1021 taccgggcga tggaggtctg gatgacctcg aggatcgcga tctgcttcgc gctgaggctc
     1081 ttgcggcggc gcgtgcgcgg agcctcggat gcgggggtgg cgttctcgct catcggtgct
     1141 ccttctcagc ggccggaccg ggcgggatcc gcgtcatcgc gggcttccgg ttcgaatgtc
     1201 ggtggccagt ggtgtgctgt ccatgtcgaa accgtatccg agaatccgcg gaaaacggaa
     1261 gatctgttcg agcgtgtcgg cgacattctc ggacgggttt cgaagaattc ttgacagatg
     1321 ttcgatatcg aagatagatt cggaacacag cttcgcatcc ggcgtccccg gccggatgcc
     1381 ggatgcgaaa gctgtaccgg tcccgccgat cgacgggaag acgaagagga gcctcacatg
     1441 agcaccatca gcatccagag ccccgcgatc cttcgcgcct ccgcaggtgc cgcctccggt
     1501 gcctccacgc gtgcggtgcg ctcgacgaag ctgcgcctca cggtgcgtgg tcggcgcgtg
     1561 ctggcggcag tcgccgcagc ccctctcgtc gcgggcatcg cattctcggt gctggcgggc
     1621 ggttcggcgc tcgcctcggg tgagcagaac gccggagtct ccttcgagac cgtgacggtg
     1681 ctcccggggg acaccctgtg gtcgatcgcg tcggaggtcg cacccggtgt cgacccgcgg
     1741 gatgtgatcg acgacatcat gagcctgaac aacctcagca gcggtgcgat ccaggccggc
     1801 tccgagatcg cgatcccgac gcagtactcg cactgaccgt catccgggga ctggcccgga
     1861 ttccgcgctc taccatggga agagtgacct ctctcgacga gctgcctctc cgcgacgatc
     1921 tgcgcgggct cacaccctac ggcgctccgc aggctcctct gcccgtggcg ctgaacgtga
     1981 acgagaacac gcatccggtg ccggagtcgg tggtcgggga catcctcgac gacatcgccc
     2041 tggcgctcag tgatgtcaac cgctaccccg accgcgagtt cacccagctg cgtgaggcct
     2101 tggccgccta cctcggccac gacctgaccg ccgaccagat ctgggcggga aacggctcca
     2161 acgaggtgct gcagcacatc atgcaggcgt tcgcagggcc gggccgcacg gccttcggct
     2221 tcgcgccgac ctattcgatg tatccgctga tcacgcaggg gaccggtgcg cgctggatct
     2281 ccggcacgcg cgagggcgac tacaccatct ctccggagga cgcggctgcg caggtcgcag
     2341 cagccgatcc cgacgtcgtg ctgctgtgct cgccgaacaa ccccaccgga acgcctctcg
     2401 ggctcgacgt ggtcgaagcc gtgtacgagg ccgcacgcgg catcgtggtg gtcgacgagg
     2461 cgtatcacga gttcgcgccg aaggacgcgg catccgctct ctccctgctg cccgggcgcc
     2521 ctcggctggc gatctcccgc accatgagca aggcgttcgc cttcgcgggc gcgcgggtcg
     2581 ggtatctggc ggccgacccc gccttcatcg acgcgctgcg actggtgcgc ctgccgtacc
     2641 acctgagcgc cctcacgcag gcggccgcga cggccgcact gcgcaactcc gtgacgatgc
     2701 tgcggatggt cgacgagatc gtcgagcagc gcgaccggat ctcggcgacc ctggaggcgc
     2761 tcggctatgc accgcacccg tcctggtcga acttcgtgct cttcggcggg atcgacgacc
     2821 ccaaggcggt gtggcaggcg ctctacgacc ggggcgtgct gatccgcgac gtcgggatcc
     2881 ccggccacct gcgggtcacg gccggcacgg aggccgagac caccgccttc ctggacgcac
     2941 tcgcatcgat aggatcacca gcatgaccgc accccgcacc gcccgccgca cccgcagcac
     3001 gtccgagtcg acggtcgagg tcgagctgaa cctcgacggc accggacgga gtgacatcag
     3061 cacttccgtg ccgttcttcg atcacatgct caccgccttc gcgaagcact cgctcaccga
     3121 cctcacggtg cgcgcgaccg gcgacacgca catcgacgcc caccacacgg tcgaggacgt
     3181 ctccatcgtt ctcggccagg cgatccgcga ggcgctcggc gacaagtccg gcatctcgcg
     3241 gtacggcgac gcgctcgtgc ccctcgacga ggccctcgcg caggccgtcg tggacatctc
     3301 cggccgtccc ttcctcgtgc acgagggcga gcccgcaggt ttcgagatgc acctcatcgg
     3361 cgggcacttc accggctcgc tcgtgcggca cgtcttcgag gccatcacct tcaacggcgg
     3421 cctcaccgtg cacgtgcgcg tggtcggcgg ccgcgacccc caccacatcg ccgaggcgga
     3481 gttcaaggcg ttcgcacgcg cgttccgcca ggccaaggcc ctcgacccgc tcgtcgacgg
     3541 cgtcccgtcc accaagggcg cgctgtgagc accggccccc gcgtcgtcgt cttcgactac
     3601 gagtccggca acgtccactc ggcggtcaag gccctggccg ccgccggagc ggatgtcgag
     3661 ctcaccggtg accgggatcg cgcgctcgag gccgacgggc tgttcgtccc cggcgtcggc
     3721 gcgttctcgg ccgtggtcga cgcgctgcgc gcgcacggcg gtgatgagat catcgaccgc
     3781 aggctcgcgg gcggccggcc ggtgctcggt gtctgcgtcg ggatgcaggt gatgttcgag
     3841 cgcggcgtgg aacgcgggct gaacaccgag ggactggggg agtggccggg cacggtcgcc
     3901 gagctcgacg ccccggtgct gccgcacatg ggctggaaca cggtcgaggc gggtgagggc
     3961 agtgtgctgt tccgtggtct cgagcaggag cgcttctact tcgtgcactc cttcgccgcg
     4021 aagacctggg ggatccaggt cgagccgccg ttcccgcagc cggcgctgac ctgggcgacc
     4081 cacggcgaga ggttcctcgc ggccgtggag aacggcccgc tgtcggcgac gcagttccac
     4141 ccggagaagt ccggggaggc gggcatccgg atgctgcgca actgggtgga atcgctcggc
     4201 tgagcgtcac ccgacgccac tcatgttgaa ccgcgcacag gcgcggacta gtgtcttctc
     4261 tcgtgtccgc acgtcgcgga catccccgaa ccaaggacgt catgaacgac ttcgcacagt
     4321 ccccctcgct gatcctgctg cccgcagtcg acgtcgcggg agggaaggcg gtgcgtctca
     4381 cccagggtga ggcaggcacg gagaccagct acggcgatcc gatcgacgcc gcaggcgagt
     4441 gggtcgacca gggtgcgcag tggatccacc tcgtggacct cgacgcggcc ttcggacgcg
     4501 gcagcaacgc gcccatcctg cgcaaggtca tcaagcagta ccgcggcgtg aacatcgagc
     4561 tctccggcgg catccgcgac gacgccagcc ttgaggccgc gctcgagtcc ggcgccaacc
     4621 gcatcaacct cggcacggcg gcgctggaga accccgagtg ggcggccgac gtcatcagcc
     4681 ggtacggcga ggcggtcgct gtcggactcg acgtgcgtgg caccaccctg gccgcacgcg
     4741 gctggaccaa ggacggcggt gacatctggg aggtgctgga gcgcctcgag gaggccggct
     4801 gcagccgtta cgtcgtcacc gacgtgacca aggacggcac gctgcgcggc ccgaacctgg
     4861 agctgctgcg tgagatcacc tcgcgcacgc ccaagccggt gatcgcgtcc ggcggcgtct
     4921 cgaacctcga cgacatcgcg gctctccgcg agctcgtgcc gctcggcgtc gagggcgcca
     4981 tcgtcggcaa ggcgctctac gccgggcagt tcacgctggc agaggcgctg gatgtcgccg
     5041 gcttctgacg gcgcctgcga ccacggggac accgaaggca acaccgcaga ctccgccggc
     5101 gtgccctggg ccggccggag cttcgagccg aacactcgcg ccgcggatga cggctcggcc
     5161 gac
//
LOCUS       DATMSX010000501        14515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_74206,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000501 DATMSX010000000
VERSION     DATMSX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74206"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VN041_16030"
     CDS             <1..838
                     /locus_tag="VN041_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU30589.1"
                     /translation="LAPVLATARYAMDRTVTDSGFGPAPWLLGVALLALCAVAVLMGL
                     LRRAELRTTALLEERLDLVRRDQDNRQRQAASAERSRISGELHDVLGHSLTVIVAQAR
                     VARFTPARTAPALEVIEETARTSLRELRQTLRTLHEGHADASSAAPVTPDALAERMRG
                     LGLTVERRVQGVPRQLNSDSELALGRFLQEGLTNALRHGTGGVEWIEDWRDDLVVIRL
                     SNPVAGDATTGDGTGLGLPAMQKRIAAVGGHVEVASDGPFTVIAVLPYAESTALGTEA
                     VR"
     gene            835..1482
                     /locus_tag="VN041_16035"
     CDS             835..1482
                     /locus_tag="VN041_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30590.1"
                     /translation="MIRILVADDQPLVRAGIVATIDAQDDMEVVGEAEDGNGAIDSAG
                     RLAPDVVLMDIRMPGVDGIEATRAVRERDPDRRVLILTTFDADDHVYAALRAGACGFL
                     VKDAPVEELVAAVRAAARGDAVLSPAITARVVEQLLAAPVPAVPSLPSGEILTPREIE
                     LVRLVADGLSNLEIGERLHLSEPTIKTHMGHVLAKLGMRDRLQVAVWVHRSGIAR"
     gene            1579..2274
                     /locus_tag="VN041_16040"
     CDS             1579..2274
                     /locus_tag="VN041_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30591.1"
                     /translation="MNDPVLNAHALTKHYGPTRALAGVDAAVHRGESLAIMGASGSGK
                     TTLLHALAGIISPDSGSVAFTSASGERIDVTGQSESVRSRLRRERFGFVFQQGLLIPE
                     LTAIENVALALMINGTKRQDAIGPAAAWLQSLGLAGMEERRIGELSGGQAQRVAIARA
                     QVTGAELIFADEPTGALDSHTSAEVMDALLWSTTGQGRTLVVVTHDSQVAARCTRVLR
                     MRDGVIVDAEVSA"
     gene            2271..3593
                     /locus_tag="VN041_16045"
     CDS             2271..3593
                     /locus_tag="VN041_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWU30592.1"
                     /translation="MNASVMRMLLRPAPGNAPTLVIPATAFAIVTALVLTVVGGAQSF
                     WTWDDEMALIYQVLAAVALVLLVVPLASLGGAAARLSARRRDERLSTLRLLGVTPAGV
                     GALTVVESTLIATAGVVAGVIGHLLLAPLIGLIPFRGEPLGLGSVLLSPAMIAVVCAG
                     AVLLAAVSAILGLRRVIVSPLGVRMRAQVTRVHWVRGVVAVLVVAAAFLTAQLVPALT
                     GVVTITVVLAGIFAGTLAVLNLVGPWALAVFSRSRLKRAQTPERLLAARTVLDDPKAA
                     WRQVGGIAMASFMAVFAGTGVSLMNVMSASDAGDSNLALVVDMRTGLIITLVASFVMV
                     AASVGVTQASDVLDQRPLHRSLHQLGVPAETVDAARRRAIMSPLLFTALGSALCAGIV
                     VFPLLGITLIVAPVSLLTIVAVLAAGILTVWLSTLATRPLLRRAFTTA"
     gene            3610..4131
                     /locus_tag="VN041_16050"
     CDS             3610..4131
                     /locus_tag="VN041_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU30593.1"
                     /translation="MILRTPRLDLREMTEEDLPALRAILQDPITMTAYEGAFDEDQVQ
                     TWLQRTLDRYRDDGYALWAVVLRETGEMIGQCGPTVQHILDEDVLEVGYLFNRAHWHR
                     GYATEAAAGVRDHAFASLGAERVYAQIRDTNIASMNVAIRLGMTVRGRFVKNYRDVDM
                     PHLAFAVDRPSAV"
     gene            complement(4120..4824)
                     /locus_tag="VN041_16055"
     CDS             complement(4120..4824)
                     /locus_tag="VN041_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30594.1"
                     /translation="MTTTSTALVTGATRGIGRAIAAALAATHHVHLIGRNRGALDELA
                     EVLPSAEVHVADLADGAQLAGIAGGIDRLDVLVHSAGVLHMGSTEDLDPAQWRESLEV
                     NVLAPVELTRVLLPALRRASGQVILINSGLGHRSIAGSGAYSASKFALRAFADALRQE
                     EAENGVRVTSIHPGRVATPMQEQLRAWEGLPYDGDAWIRPEQVADAVLTAVNLDRNAV
                     IGTLDISPTPRARSDR"
     gene            4908..6065
                     /locus_tag="VN041_16060"
     CDS             4908..6065
                     /locus_tag="VN041_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30595.1"
                     /translation="MTGAPDDLDLLAAGDPATPPQRLADIAARRHDLHAVIAANPSTY
                     PELRQWIGQVNPGYAQQIAVPSTSVVPPYAGAPYGSGFAAPPVAPRRKGLGWIFAGCG
                     CLTLVAGIVIAAVVIGGLGASTAGGGGSDGGRPTASTVDEQLAVIRTESTEYEKLAAQ
                     LDGNPVAPLVAQPTRFQRLQADAEALKASTALEKVRQAQARTLAERATKYRSDLQRLI
                     TAAEGRRANASGTSEEQLVDSAGNGFIDIQWNADSECSTSENPESTTAGCVTNTPEQL
                     IVHILPREKMRGGDEAARITTLHELAHLYQHTDVETTADGEVGPATKLKEAGLFQGSG
                     EVMSDCYALTYLGLNSLTVGGVTLGYGYVCDDSERQAIRDWAKAIHAPMPG"
     gene            complement(6113..7492)
                     /locus_tag="VN041_16065"
     CDS             complement(6113..7492)
                     /locus_tag="VN041_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWU30596.1"
                     /translation="MSVRIVLSHIEERQCVEVSVNPIVVIGAGPQGLAAAAHLLERGL
                     VPLVLESGDAAASATAEWGHVRLFSRWAELIDPASRRLLEPTGWTAPVQGYPTGAQWI
                     EHYLAPLAAVLGDRVRYGARVVGVGRKGRDLSVDAGRADQPFVIHVERSDGAEERIEA
                     SAVIDASGTWRMPNPAGADGLPALGERAAADAGVLAHRMPASEEASALAGRHVVVVGN
                     GHSAATTIGMLSRVAKSAPGTRVTWVLRRGAVGNTFGGGSSDELPERGALGQRAKDAV
                     DSGLVRLVTGFRTEQIVLDGGQAVLVAEDGRRLEPAARIFVATGFRPDLSFLSEMRVD
                     LDVRLQAPAKIAAEVDPNVHSCGSVRATGAADLQQPEPGFYIVGAKSYGRAPTFLALT
                     GFEQVRSVVAAIAGDREAAERVELVLPDTGVCGGSGLFDAPGDQSGSGSCCVAEPQLI
                     QLGRAPAAR"
     gene            7529..7870
                     /locus_tag="VN041_16070"
     CDS             7529..7870
                     /locus_tag="VN041_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWU30597.1"
                     /translation="MNALPVTIDIASGSCCTPGEAMDDDAAQGLAKVFKALGDPTRVK
                     LLSMIAGSSAGELCVCDLTEPVGLSQPTVSHHMKLLVEAGLVTREQRGKWAYYRPTTG
                     TLADAAKALIG"
     gene            7894..8415
                     /locus_tag="VN041_16075"
     CDS             7894..8415
                     /locus_tag="VN041_16075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU30598.1"
                     /translation="MSPSDWPDVEQIYRAGIATGQATFESEPPPSWQAFASGKLQHLM
                     LVALEDSIVVGWAAAGPVSARAVYRGVVEHSVYVAPRAAGRGVGRLLLAELIRAAERS
                     GVWTIQSSVFPENTASLRLHERAGFRRVGRRERIALMSYGPFAGRWRDTVLIERRSDD
                     PPVTVPASGAPLS"
     gene            complement(8506..9405)
                     /gene="mutM"
                     /locus_tag="VN041_16080"
     CDS             complement(8506..9405)
                     /gene="mutM"
                     /locus_tag="VN041_16080"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWU30599.1"
                     /translation="MPELPEVEVVRSGLAPAVVGARVTSVDVLDERALTRHPLGAADF
                     VGRLEGRVVTAAARRGKFLWLPLEGETDAVVAHLGMSGQMLLRAPDAVAERHERVRLH
                     IEHPQHGRLAVVFADQRTFGSLAIDGLVPTGDDAAGGFGTDRALVPSQVVHIARDALD
                     PAFDDARFTASVRRKASAIKRTLLDQTLVSGIGNIYADESLWAARIHPETEGRALSSQ
                     AVRRLLAEVRLVLQKALAEGGTSFDAQYVNVNGQAGYFAHSLNAYGRGGEACPRCGGP
                     IRRTAFMNRSSHFCPRCQRRRSS"
     gene            complement(9414..10124)
                     /gene="rnc"
                     /locus_tag="VN041_16085"
     CDS             complement(9414..10124)
                     /gene="rnc"
                     /locus_tag="VN041_16085"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWU30600.1"
                     /translation="MAEDSAGAQALIEKLGVDIDAELLERALTHRSYAYEHGGIPHNE
                     RLEFLGDSVLGIAVTVMLFTTHPDLDEGELAKRRASVVSTVALAEVARAIGLGAHLRL
                     GRGEEQTGGRDKDSILADTMEAVFGATYLSAGAKAAEALVLRLTEPLLADPERYGAAM
                     DPKTSLQELAARLGRTPPEYTVESSGPDHNRVFTATVTVGELASTGQGTSKKTAEMAA
                     ALTAWRELSLRVTESGAA"
     gene            complement(10135..10344)
                     /gene="rpmF"
                     /locus_tag="VN041_16090"
     CDS             complement(10135..10344)
                     /gene="rpmF"
                     /locus_tag="VN041_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWU30601.1"
                     /translation="MAGNPPKRKVSRSNTRSRRAQWKAAPVALVKTVENGQVVYSRPH
                     QAKVVTDSQGTELFLEYKGRKVADV"
     gene            complement(10347..10841)
                     /locus_tag="VN041_16095"
     CDS             complement(10347..10841)
                     /locus_tag="VN041_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWU30602.1"
                     /translation="MREHRFSVTLADKWGKGIVVVEAGESVDLDVRLESVHEGILVSG
                     TADSDYVGVCGRCLTDITAPVEVEFQELFAYPGEEETDFEVQDDHVDLETLVRDAVVL
                     SLPFQPVCQPDCPGLDPSTGEKLTVSTDAESTAPIDPRWSALRQITDQDAPAGSRGAE
                     KEES"
     gene            complement(10965..11462)
                     /gene="coaD"
                     /locus_tag="VN041_16100"
     CDS             complement(10965..11462)
                     /gene="coaD"
                     /locus_tag="VN041_16100"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWU30603.1"
                     /translation="MNSRIAVVPGSFDPPTLGHLDVIRRAARLYEELHVLVVHNPGKA
                     AMLPIAQRLSLLEQSIAEAGIEGHIVVGSWSMGLLVDYARDVDAGVLVKGIRSQVDVA
                     YESPMAIVNRHLADIETVFLLPDPAHALVSSSLVRQVASLGGDVSPFVPPAVAAFLDT
                     GARGI"
     gene            complement(11488..13659)
                     /locus_tag="VN041_16105"
     CDS             complement(11488..13659)
                     /locus_tag="VN041_16105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWU30604.1"
                     /translation="MSFTLETPLAEAVGTSTAKTLERAFGMGTVGELLGHYPRRYADR
                     GELTPILELPVGETVTIVAEVLSTRARQMRNRRGAMLEVIIGDGVGQMSLTFFAKNLG
                     QAEWRAKELRVGRRGIFSGKVSQFRDTVQFAHPDYQLFEDSEAAYRQADALQDVPIPI
                     YPATSAMQSWQIAKVIDAVLDKLGEVPDPLPEDLRRREGLLTAGEALQSIHRPHRRAD
                     VDPAVRTLRMHEALVLQTALLQQRNLVRSLIAQPRPAHAGGLLEQFDAKLPYTLTADQ
                     QRVGAEIADDLLLERPMNRLIQGEVGSGKTLVALRAMLQVAESGGQAALIAPTEVLAA
                     QHLRSITKMLGPDLAPQLIPTLLTGQMSAPERRKAALRVASGQSLLVIGTHALLSEKT
                     TFADLALVVVDEQHRFGVEQREMLRAKGTAPHAIVLTATPIPRTVAMTVFGDLDTSVI
                     RSMPAGRAGISTFVAPLAEHPEWFQRVWERSAEEIAQGRQVFAVCAAIDAAEDSVESD
                     DAPPPDVEGKGPRWGVVQLEQLLATHPRLGEVRRAILHGKMPADEKDATMRAFARGEI
                     DLLVATTVIEVGVDVPNASTMIIMDADRFGVSQLHQLRGRVGRGEFPGLCLLVTEAEG
                     GSIARERVEAVAATLDGFELAEVDLELRGEGDVLGAAQAGAKSSLRLLRVVKDAGLIA
                     HARDIAADILKDDPMLVKHPGLRVAIAGRVSDADRAALSKN"
     gene            13724..14287
                     /gene="rsmD"
                     /locus_tag="VN041_16110"
     CDS             13724..14287
                     /gene="rsmD"
                     /locus_tag="VN041_16110"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWU30605.1"
                     /translation="MTRIIAGAARGIRIDVPDAGTRPTSDRVRESVFGALEAMDAIDS
                     ARVLDLYAGSGALGLESWSRGAASVDLVERSRPAATIIGRNTAAVAKAVGGPNAAKVH
                     QSAVHAFLGRATGTYDLVFTDPPYDLDDSAMTADLVALAPLLSADAIVVIERAKRSTA
                     PDLDAAGLELVRERTYGDTAVWWAQPA"
BASE COUNT         2140 a         5138 c         5039 g         2198 t
ORIGIN      
        1 gctcgcgccc gtcctggcca ccgcgcggta cgccatggac cggacggtga ccgacagcgg
       61 tttcgggccg gctccctggc tgctgggcgt cgccctgctg gcgttgtgcg cggtcgccgt
      121 cctgatggga ctgctgcgcc gggcggagct gcgcaccacg gcgctgctcg aggaacggct
      181 cgatctcgtc agacgcgatc aggacaaccg ccagcgacag gccgccagcg ccgagcgctc
      241 ccggatctcg ggcgaactgc acgacgtact cggacactcc ctcaccgtca tagtcgccca
      301 ggcgcgcgtc gcccggttca cccccgcccg cacagccccg gcactggagg tgatcgagga
      361 gacggcccgc acctcgctcc gcgagctgcg gcagacgctg cggacactcc acgagggcca
      421 tgcggatgcc tcttccgcag cacccgtgac cccggacgca ctggccgagc ggatgcgcgg
      481 cctgggcctg accgtggagc gtcgcgtcca gggtgtccct cgtcagctga actcggactc
      541 tgagctcgcg ctcggacgct tcctgcagga ggggctcacg aacgccctcc ggcacggcac
      601 cggcggcgtc gagtggatcg aggactggcg agatgatctg gtcgtcatcc ggttgagcaa
      661 ccctgtggcc ggcgacgcga cgacgggcga cgggacgggc cttggcctcc cggcgatgca
      721 gaagcggatc gccgcggtgg gcggtcacgt cgaggtcgca tccgacggac ccttcaccgt
      781 catcgccgta ctcccctacg cggagtccac tgctctgggc acggaggcgg tccgatgatc
      841 cgcatcctgg tcgccgacga tcagccgctg gtgcgtgcgg gcatcgtcgc gacgatcgat
      901 gcgcaggacg acatggaagt cgtcggggag gccgaagacg gcaacggcgc gatcgactcc
      961 gccggcagac tcgcgccgga tgtcgtgctg atggacatcc ggatgcccgg ggtcgatggg
     1021 atcgaggcca cgcgtgcggt gcgggaacgc gatcctgatc ggcgggtcct catcctcacc
     1081 acgttcgacg ccgacgatca cgtgtatgcc gcactccggg caggggcgtg cggattcctc
     1141 gtgaaggacg cccccgtgga ggagctggtc gccgcggtgc gcgcggccgc acgcggagac
     1201 gccgtgctct cgcccgcgat cactgcgcgc gtggtcgagc agctgctggc cgcaccggtt
     1261 ccggccgtgc cgtctctccc ctcgggcgag atcctcactc cacgggagat cgagctggtg
     1321 cgcctggtcg cagacggcct cagcaacctg gagatcggcg aacggctgca cctgtccgaa
     1381 cccaccatca agacccacat gggtcatgtc ctggccaagc tcgggatgcg cgatcggctc
     1441 caggtggccg tgtgggtgca ccgttcaggc atcgcccggt gaggcggacg gcggatcatc
     1501 cgagaggatg accgctccgc acccgagtca tcctccgggc cgacgacgtg cgcatgcccc
     1561 ggatcggatg ctggaatcat gaacgatccg gtcctgaacg cgcacgcact cacgaagcac
     1621 tacggcccca cccgcgctct ggcaggcgtc gacgccgccg tccaccgcgg ggagtccctc
     1681 gccatcatgg gcgcgtcggg ctccggcaag accacgctgc tgcacgcgct ggcgggcatc
     1741 atcagccctg acagcgggtc ggtcgcgttc acgtcggcat ccggtgagcg catcgacgtc
     1801 accggccagt ccgaatctgt gcggtcgcgg ctgcgacggg agcggttcgg gttcgtgttc
     1861 cagcaggggc tgctgatccc ggagctcacc gccatcgaga acgtcgcgct cgcgctgatg
     1921 atcaacggca cgaagcgcca ggacgccatc ggcccggcgg ccgcctggct gcagtcgctg
     1981 ggcctcgccg ggatggagga gcgccgcatc ggcgagctct ccggcgggca ggcccagcgg
     2041 gtcgcgatcg cccgcgctca ggtgaccggg gcggagctga tcttcgccga cgagccgacc
     2101 ggagctctgg actcgcacac ctctgcggag gtgatggacg cgctcctctg gtcgaccacc
     2161 ggccaggggc gcacgctcgt ggtcgtcaca cacgactcgc aggtggcggc gcgctgcacg
     2221 cgcgtcctgc ggatgcgcga cggcgtgatc gtggatgcgg aggtgtcggc atgaacgcat
     2281 cggtgatgcg gatgctgctg cgccccgcac cggggaacgc accgacactc gtgatccccg
     2341 ccacggcgtt cgcgatcgtc acggctctcg tgctgacagt cgtaggcggc gcgcagtcgt
     2401 tctggacgtg ggacgacgag atggcactca tctatcaggt cctcgcggcc gtggcgctgg
     2461 tgctgctggt ggtccccctg gcgtccctgg gcggtgccgc cgcccgcctc tcggcccgtc
     2521 gccgcgacga gcgactctcc acccttcggc tgctgggcgt gactcccgca ggcgtgggcg
     2581 cgctgaccgt cgtcgaatcg accctcatcg ccaccgccgg cgtcgtggcg ggcgtcatcg
     2641 ggcatctgct gctcgcgccg ctgatcggtc tgatcccctt ccgcggcgag ccgctcggac
     2701 tcgggtccgt gctgctctcg cccgccatga tcgccgtcgt gtgcgccggc gcggtgctgc
     2761 tggccgcggt cagcgcgatc ctgggactcc ggcgcgtgat cgtctcaccg ctcggagtgc
     2821 gcatgcgcgc ccaggtcacc cgggtgcact gggtgcgcgg cgtcgtcgcg gtgctcgtgg
     2881 tggcggccgc attcctcacc gcccagctgg tccccgccct caccggcgtc gtgacgatca
     2941 ccgtcgtgct cgccggaatc ttcgccggca cgctcgcggt gctcaacctc gtgggcccgt
     3001 gggcgctcgc cgtgttctcc cgcagccggc tcaagcgggc gcagacccct gagcgcctgc
     3061 tcgccgcgcg cacggtgctc gacgatccga aggcggcgtg gcgccaggtc ggaggcatcg
     3121 cgatggcgag cttcatggcc gtgttcgccg gcaccggcgt gtcgctgatg aacgtcatga
     3181 gcgcgtcgga tgccggggac tcgaacctcg ctctggtcgt ggacatgcgc accggcctca
     3241 tcatcacgct ggtggcgtcg ttcgtgatgg tcgcggcatc cgtcggggtg actcaggcct
     3301 ctgacgttct cgaccagcgt ccgctgcacc gcagcctgca ccagctcggc gtccccgccg
     3361 agacagtcga cgcggcacgg cgtcgcgcga tcatgtcgcc cctgctgttc accgccctgg
     3421 gatccgcatt gtgtgccggg atcgtcgtgt tcccgctgct ggggatcacg ctgatcgtcg
     3481 cgcccgtctc gctgctgacg atcgtggccg tgctcgcagc cggcatcctc accgtgtggc
     3541 tcagcacgct cgccaccagg ccgctgctgc ggcgcgcgtt cacgacggcg tgagtgagag
     3601 gatcgaagga tgatcctccg cacgccccgc ctcgacctcc gtgagatgac cgaagaggat
     3661 ctgcccgcgc tgcgtgcgat cctgcaggat ccgatcacga tgaccgccta cgagggcgcg
     3721 ttcgacgagg atcaggtgca gacctggctg cagcggacat tggatcggta ccgcgacgac
     3781 gggtacgccc tgtgggccgt ggtcctgcgc gagaccggcg agatgatcgg acagtgcggg
     3841 cccacggtgc agcacatcct cgacgaggac gtgctcgagg tcgggtatct cttcaatcgg
     3901 gcgcattggc accgggggta cgcgaccgag gcggccgcgg gcgtgcgcga tcatgccttc
     3961 gcgtcactcg gcgccgagcg cgtgtacgcc cagatccgcg acaccaacat cgcctcgatg
     4021 aacgtcgcga tccggctcgg catgaccgtt cgcggccggt tcgtgaagaa ctatcgcgac
     4081 gtcgacatgc cgcacctggc cttcgcggtg gaccgtccgt cagcggtctg aacgggctct
     4141 cggcgtcgga ctgatgtcga gggtcccgat caccgcgttg cgatcgaggt tcaccgcggt
     4201 gagcacggca tccgccacct gctccggccg gatccaggcg tcaccgtcgt acgggagccc
     4261 ctcccaggca cggagctgct cctgcatcgg cgtggcgaca cgaccgggat ggatggaggt
     4321 gacgcgaacg ccgttctccg cctcctcctg tcgcagcgca tcggcgaacg cgcgcaaggc
     4381 gaacttgctc gcgctgtatg ccccggaacc ggcgatcgag cggtggccga gcccggagtt
     4441 gatcagaatc acctgccccg acgcgcgccg cagtgcgggg agcagcacgc gcgtcagctc
     4501 cacgggcgcg agcacgttga cctcgaggct ctcgcgccac tgggcggggt cgaggtcctc
     4561 ggtcgagccc atgtgcagga ctccggcgct gtgcacgagc acatcgagcc ggtcgatccc
     4621 gcctgcgatt ccggcgagct gcgcgccgtc ggcgaggtcg gccacgtgca cctcggccga
     4681 tggcagcacc tccgcgagtt cgtcgagcgc tccgcggttg cgcccgatca ggtgaacatg
     4741 gtgcgtcgcc gcgagtgcgg cggcgatcgc acggccgatt cccctggtcg caccggtgac
     4801 gagggctgtg ctggtcgtgg tcatcctccc agcctgtcac agcgcatctc ccgccggcgc
     4861 atgggccgcg cccgagcggc acgtcgcctg tggttaggct tgcggacatg accggagccc
     4921 ctgacgacct cgatctcctc gccgccggcg atcccgccac tccccctcag cgactcgccg
     4981 acatcgccgc ccgcaggcac gatctgcacg cggtcatcgc cgccaacccg agtacctacc
     5041 ccgaactgcg tcagtggatc ggacaggtga accccggcta cgcgcagcag atcgccgttc
     5101 cgtcgacctc ggttgtcccg ccctacgcag gcgcaccgta cgggagcggt ttcgcagcac
     5161 caccggtcgc accgcgccgc aaggggctcg gctggatctt cgccggctgt ggatgcctga
     5221 ccctcgtcgc ggggatcgtg atcgccgccg tcgtgatcgg cgggctgggc gcctccaccg
     5281 cgggcggtgg agggtcggac ggcggccgtc ccaccgcctc gacggttgac gagcagctcg
     5341 ccgtcatacg caccgagagc acggagtacg agaagctcgc ggcccagctc gacggcaacc
     5401 ccgtcgcccc gctggtcgct cagcccacca ggttccagcg gctccaggcc gatgcggaag
     5461 cgctcaaggc gagcacggcc ctcgagaagg tcagacaggc gcaggcgcgg acgctcgccg
     5521 agcgggcgac caagtaccgc tccgatctgc agcggctcat caccgccgcg gagggccgtc
     5581 gcgcgaacgc gtccgggacc agcgaggagc agctcgtcga cagcgcgggg aacggcttca
     5641 tcgacatcca gtggaacgcg gactcggagt gcagcacgtc cgagaacccg gagagcacga
     5701 cagcgggctg cgtgacgaac acccccgagc agctgatcgt gcacatcctc ccacgcgaga
     5761 agatgcgcgg cggggacgag gccgcccgca tcacgacact gcacgagctg gcgcacctgt
     5821 accagcacac cgatgtggag accaccgccg acggcgaagt gggaccggcg acgaagctca
     5881 aggaggccgg gctgttccag ggcagcggcg aggtcatgtc ggactgctac gccctcacct
     5941 acctcggcct gaactcgctc accgtcggag gcgtcacgct cggctacggc tacgtctgcg
     6001 acgactccga gcggcaggcg atcagggact gggcgaaggc gatccacgcc cctatgccgg
     6061 gctgatcgag gtgggagcag gtcgattccc accccgttcc cgctgccggc gcctaccgcg
     6121 cggcgggcgc cctacccagc tggatgagct gcggttcagc tacgcagcag gatcccgagc
     6181 ctgattggtc gccgggtgca tcgaacagcc ccgagccgcc gcacacgccg gtgtcgggga
     6241 ggaccagttc cacgcgctcc gcggcctcgc ggtcgccggc gatcgctgcg acgacgctgc
     6301 gcacctgctc gaacccggtg agggcgagga acgtcggcgc gcggccgtac gacttcgcgc
     6361 cgacgatgta gaagcccggc tcgggctgct ggagatcggc cgcgccggtg gcgcggaccg
     6421 acccgcagga gtgcacgttc gggtcgacct cggcggcgat cttcgcgggt gcctggagcc
     6481 gcacgtcgag atcgacacgc atctcagaca ggaaggacag gtcgggtcgg aaccctgtgg
     6541 cgacgaagat ccgcgctgcg ggctccagtc tgcgcccgtc ctccgccacg agcactgcct
     6601 gccctccgtc gaggacgatc tgctcggtgc ggaaccccgt cacgaggcgg acgagtcccg
     6661 aatcgacggc atccttggcc cgctgcccca gggcgccgcg ctcgggcagc tcatccgaac
     6721 tgccgccgcc gaaggtgttt ccgacggcgc cgcgacgcag cacccaggtg acccgcgtcc
     6781 cgggggcgct cttggccacc cgtgagagca tgccgatggt cgtcgcagcc gagtgaccgt
     6841 tgccgaccac gacgacgtgt cgacccgcga gcgcgctcgc ttcctcggat gcgggcatgc
     6901 ggtgcgcgag cacaccggcg tccgccgccg ctctctcgcc gagagcagga agcccgtcag
     6961 caccggcagg gttgggcatc cgccaggttc ctgacgcgtc gatcacggcg ctcgcctcga
     7021 tccgctcctc tgcgccgtcc gaccgctcga cgtggatgac aaaaggctga tcggcgcggc
     7081 ctgcatcgac ggagaggtcc cggcctttgc gccccacgcc caccacgcgc gcaccgtacc
     7141 ggacgcgatc gccgaggacc gcggcgagag gtgcgaggta gtgctcgatc cactgggcgc
     7201 ccgtcggata tccctggacg ggggcggtcc agccggtcgg ctccaacaga cggcgcgatg
     7261 cggggtcgat gagctccgcc cagcgagaga acagccgcac gtggccccat tcggcggtgg
     7321 ccgaggcggc agcgtcgccc gattccagca ccagcggcac gagcccgcgc tcgagcaggt
     7381 gagcggcggc ggccagcccc tgggggccgg cgccgatgac gacgatggga ttcacggaga
     7441 cctccacgca ttgacgttct tcgatgtgag acaggacgat cctgacgctc atatcgacag
     7501 atgtcaatac gtggggcaga ctgtacccat gaatgccctg ccggtcacca tcgacatcgc
     7561 ctccgggtcg tgctgcacgc ccggtgaggc gatggacgac gacgcagcgc agggcctggc
     7621 gaaggtcttc aaggcgctgg gagatccgac ccgggtgaag ctgctctcga tgatcgcggg
     7681 aagcagtgcc ggggagttgt gcgtctgcga cctcacggaa cccgtgggtc tgtcccagcc
     7741 gacggtctcc caccacatga agctcctcgt ggaggcgggc ctggttaccc gcgagcagcg
     7801 cggcaagtgg gcttactaca ggcccaccac cggcacgctc gccgacgccg cgaaagcact
     7861 catcggctga tggggacgat cgggatccgg cccatgtcgc cgtccgactg gccggacgtc
     7921 gagcagatct accgcgccgg gatcgcgaca gggcaggcca cattcgagtc ggagccgccg
     7981 ccgtcatggc aggccttcgc gtcaggcaag ctgcagcatc tcatgctcgt cgctctcgag
     8041 gactcgatcg tcgtgggttg ggcggcggca ggtcccgtct ccgcccgcgc cgtgtaccgc
     8101 ggggtggtcg agcattcggt ctacgtcgcg ccccgagccg ccggccgcgg cgtcggtcgc
     8161 ctgctgctcg cagaactgat ccgggctgcc gaacgctcag gcgtctggac catccagtcg
     8221 tcggtgttcc ccgagaacac cgccagcctc cgccttcacg agcgcgccgg cttccggcgc
     8281 gtgggacggc gcgagcgaat cgcgctcatg tcgtacggtc ccttcgcggg ccggtggcgc
     8341 gacacggtcc tgatcgaacg tcggtctgac gatccgccgg tcaccgtacc ggcctccggc
     8401 gccccgctga gctgagtcct cgccaccgtg atgtgcatgg tgcctcacgc gggggacgcg
     8461 tgcgcgacac tgcttgagat cctctgccga caggtcggtt gaggatcagg aggagcgcct
     8521 gcgctgacag cgcgggcaga aatgactcga gcggttcatg aacgccgtcc gacggatcgg
     8581 tccgccgcag cgcgggcacg cctctccccc gcgaccatag gcgttcaggg agtgggcgaa
     8641 gtaaccggcc tgcccgttca cgttcacgta ctgggcgtcg aaactggtgc cgccctcggc
     8701 gagggccttc tgcagcacca gccgcacttc ggccaggagc cgacgcacgg cttgggatga
     8761 gagtgcgcgg ccttcggtct cggggtggat ccgcgctgcc cacagcgact cgtccgcata
     8821 gatgttcccg attccgctga ccagcgtctg gtcgagcagc gtgcgcttga tggcgctggc
     8881 cttacggcgc acggatgcgg tgaagcgggc atcgtcgaat gcgggatcga gggcgtcccg
     8941 cgcgatgtgc acgacctggg acgggacgag cgcgcggtcc gtgccgaagc cacccgccgc
     9001 atcgtctcct gtgggcacga gtccgtcgat cgccagcgag ccgaacgtcc gctgatcggc
     9061 gaacacgaca gccaggcgtc cgtgctgagg gtgctcgatg tgcagacgga cgcgctcgtg
     9121 acgctccgcc acggcatccg gcgcgcggag cagcatctgg ccgctcatgc cgagatgcgc
     9181 gaccacggca tccgtctccc cctccagggg aagccagagg aacttgccgc ggcgcgccgc
     9241 cgctgtcacg acacgaccct cgaggcgccc gacgaagtcg gcggcgccga gcggatgccg
     9301 ggtcagcgcg cgttcgtcga ggacgtccac cgaggtgacg cgcgcgccga cgaccgccgg
     9361 cgcaagcccg gatcggacga cctcgacctc aggaagctcg ggcacggcgc gattcaggcg
     9421 gcgccggact cggtgacgcg cagactcagc tcgcgccagg cggtgagagc tgcggccatc
     9481 tcggcggtct tcttgctggt gccctgtccg gtgctggcaa gctcgccgac cgtgaccgtc
     9541 gccgtgaaga cacggttgtg gtcgggcccc gacgactcca cggtgtactc cggtggcgtg
     9601 cgccccagac gcgcggccag ctcctgcagg ctcgtcttgg ggtccatcgc cgcgccgtag
     9661 cgctcggggt cggccagcag cggctcggtc agtcgcagca cgagagcctc ggcggccttc
     9721 gccccggcgg acagataggt cgcaccgaag acggcctcca tggtgtccgc gaggatcgaa
     9781 tccttgtcgc ggccgccggt ctgctcttcg ccgcgtccca gccgaaggtg cgcgcccaac
     9841 ccgatggcac gggccacctc ggccagcgcg acggtcgaga cgacgctggc gcgccgcttg
     9901 gcgagctcgc cctcgtcgag atccgggtgc gtcgtgaaca gcatcaccgt cacggcgatg
     9961 cccagcacgg agtccccgag gaactccagc cgttcgttgt gcgggatccc gccgtgctcg
    10021 tacgcgtacg aacgatgcgt cagtgcccgc tcaagaagct cggcgtcgat atcgacgccg
    10081 agcttctcga tgagagcctg tgcccccgcg gaatcctccg ccatgatcgc ctgatcagac
    10141 gtcggcgacc ttgcggccct tgtactcgag gaacagctcg gtgccctgcg agtcggtgac
    10201 gaccttcgcc tggtgcgggc ggctgtagac gacctgaccg ttctcgacgg tcttcaccag
    10261 ggcgacggga gccgccttcc actgcgcacg acggctgcgg gtgttggaac gggagacctt
    10321 gcgcttcggg gggttaccgg ccatgactag ctctcttctt tctcggcgcc gcggctccct
    10381 gccggggcgt cctggtctgt gatctggcgg agcgcgctcc atcgaggatc gatgggagcg
    10441 gtgctctctg cgtcggtgct cacggtcagc ttctcgcccg tgctcgggtc gagacccggg
    10501 caatccggct gacacaccgg ctgaaatgga agcgacaata cgaccgcatc cctgaccaga
    10561 gtttcaagat ccacgtggtc gtcttgaact tcgaagtcag tttcttcctc accaggatac
    10621 gcgaaaagct cctggaactc gacttcgaca ggcgcagtga tgtcggtgag gcagcgaccg
    10681 cacactccta cgtagtcgga atcggccgtt ccggagacca gaatgccttc gtggaccgac
    10741 tccagacgca cgtcgaggtc gacgctctcg cctgcttcga cgaccacgat gcccttcccc
    10801 cacttgtcgg caagggtcac cgagaagcgg tgctcgcgca tctcccccgg cttgcgggtg
    10861 atgtcgcgga cgggcaggac gaaaggtccg ttgactcggg tcttcacctg acgattctac
    10921 cggctcggat gctgtgaagg cccgatgcgc tcgggccggg cccctcagat gccccgcgca
    10981 cccgtgtcga ggaacgccgc gaccgccggg ggcacgaacg gcgagacgtc gccgccgagc
    11041 gacgccacct gccgcacgag cgagctggag acgagggcgt gcgccgggtc cggcagcagg
    11101 aacaccgtct cgatgtcggc gaggtgccgg ttcacgatcg ccatcggcga ctcgtaggcg
    11161 acgtcgacct gggagcggat gcctttgacc agcacccccg cgtccacgtc gcgcgcgtag
    11221 tcgaccagga ggcccatgct ccacgagccg acgacgatgt gcccctcgat gccggcctcg
    11281 gcgatcgact gctccagcag gctcagacgc tgcgcgatcg gcagcatggc cgccttgccc
    11341 gggttgtgca ccacgagcac gtgcagttcc tcgtacaggc gcgccgcgcg gcggatcaca
    11401 tcgaggtgac cgagcgtagg cgggtcgaag gaaccgggga cgacggcgat ccgactgttc
    11461 acgctctcag cctaggcccc cggaccctca gttcttgctg agagccgcac ggtcggcgtc
    11521 gctgacgcgt cccgcgatcg ccacgcgcag tccggggtgc ttcacgagca tgggatcgtc
    11581 cttcaggatg tcggcggcga tgtcgcgcgc gtgcgcgatg agtccggcat ccttcaccac
    11641 tctcagcagc cgcagggacg acttcgctcc ggcctgcgcc gcgccgagca cgtcgccctc
    11701 gccgcgcagc tccaggtcga cctcggccag ctcgaagccg tcgagtgtcg cggccaccgc
    11761 ctccacccgc tcacgcgcga tcgagccgcc ttcggcttcg gtgacgagca ggcacagccc
    11821 cgggaattcc ccacggccga cgcgaccgcg cagctggtgc agctgggaga cgccgaatcg
    11881 gtcggcatcc atgatgatca tcgtcgaggc gttcggcacg tcgaccccga cctcgatcac
    11941 tgtcgtcgcg acgagcaggt cgatctcccc gcgcgcgaac gcccgcatcg tggcgtcctt
    12001 ctcatcggcg ggcatcttgc cgtgcaggat cgcgcgacgc acctcgccga gccgaggatg
    12061 cgtggccagg agctgctcga gctgcacgac gccccagcgc gggcccttgc cctcgacgtc
    12121 gggcggcggc gcgtcatcgg actcgactga gtcctcggcg gcgtcgatcg ccgcgcagac
    12181 agcgaacacc tggcggccct gtgcgatctc ctccgccgac cgctcccaga cccgctggaa
    12241 ccactcgggg tgctcagcga ggggtgcgac gaacgtgctg attcccgccc tgccggccgg
    12301 catcgagcgg atcaccgagg tgtcgaggtc gccgaacacc gtcatcgcga cggtgcgcgg
    12361 gatcggtgtg gccgtcaaca cgatcgcgtg cggggcggtg cccttggcgc gcagcatctc
    12421 gcgctgctcg acgccgaagc ggtgctgctc gtcgacgacc acgagtgcga ggtcggcgaa
    12481 ggtggtcttc tccgagagca gcgcgtgcgt gccgatcacc aggagcgact gcccggatgc
    12541 cactcgcagg gcggccttgc gccgctcggg cgcggacatc tgcccggtca gcagagtggg
    12601 gatgagctgc ggcgccagat ccggcccgag catcttggtg atcgaccgga ggtgctgggc
    12661 ggcgagcacc tccgtcggcg cgatcagggc ggcctgtccc ccggattccg cgacctgcag
    12721 catcgcccgc agcgccacca gcgtcttgcc ggaaccgacc tcgccctgga tcagccggtt
    12781 catcgggcgc tccagcagca ggtcgtcggc gatctcggca ccgacgcgct gctgatccgc
    12841 cgtgagcgtg tacgggagct tcgcgtcgaa ctgctcgagc agaccgcccg catgcgccgg
    12901 gcgcggctgc gcgatcagcg atcggacgag gttgcgctgc tgcaggagcg ccgtctgcag
    12961 cacgagcgcc tcgtgcatcc gcagcgtgcg gacggccgga tcgacgtcgg cgcgacggtg
    13021 cgggcgatgg atgctctgca gcgcctcccc cgcggtcaga agtccctcgc gacgacgcag
    13081 gtcctcggga agcgggtccg gcacctcgcc gagcttgtcg aggacggcgt cgatcacctt
    13141 ggcgatctgc cagctctgca tcgcggaggt cgccgggtag atcgggatcg gcacgtcctg
    13201 cagggcatcg gcctgacggt acgcggcctc actgtcctcg aagagctggt agtcgggatg
    13261 cgcgaactgc acggtgtcgc ggaactggga gaccttgccg gagaagatgc cccggcgtcc
    13321 cacccggagc tcctttgcgc gccattccgc ctggccgagg ttcttcgcga agaacgtcag
    13381 cgacatctgc ccgacgccgt cgccgatgat cacctcgagc atcgcccctc gacgattgcg
    13441 catctgccgc gcccgggtcg agagcacctc ggcgacgatc gtgacggtct cgccgacggg
    13501 cagctcgagg atcggggtga gctcgccgcg gtccgcgtag cgacgcggat agtgcccgag
    13561 cagctcgccg acggtaccca tcccgaaggc gcgctcgagg gtcttcgccg tagatgtgcc
    13621 gacggcctcg gccagcggcg tctcgagggt gaaggacatg ctccgagtct agggagcgcc
    13681 acggaccccg gccgacgccc ctgccgcccg atatcgtgaa cgggtgacca ggatcatcgc
    13741 cggcgccgcc cggggcatca gaatcgacgt gccggacgcc ggcactcggc cgaccagcga
    13801 ccgcgtgcgc gagtcggtgt tcggcgcgct ggaggcgatg gatgccatcg acagtgcgcg
    13861 cgtgctcgac ctctacgccg gcagcggcgc cctggggctg gagtcctgga gccgtggcgc
    13921 ggcatccgtc gatctggtcg agcgctcgcg cccggctgcg acgatcatcg gacgcaacac
    13981 ggcagcagtc gccaaggcgg tcggcgggcc gaacgctgcc aaggtgcacc agagcgcggt
    14041 gcacgcattc ctggggcggg cgacggggac ctacgacctc gtcttcacgg atccgcccta
    14101 cgacctggac gactcggcga tgaccgcgga tctggtcgcg ctggcgccgc tgctgtcggc
    14161 ggacgccatc gtcgtgatcg agcgcgcgaa gcgctccacc gcgccggatc tggatgccgc
    14221 ggggctcgag ctcgtccgcg agaggacgta cggcgacacg gcggtgtggt gggcgcagcc
    14281 ggcctgatcg ccccttccac aggctcaggg cccctgggac ttcatggtgc cgcgtgtcag
    14341 ccgacgacgc cgtcccagtc gcgataaggg tcccagcccg tgtgggcgat gggtgcgccg
    14401 tccagcagca cgccgtccgc gccccgcgcg gtcacgctcc cgagcgcacg gaagccctcc
    14461 ggaagcgggg cgtctgcggg gaagaccgcc agcagaccgt ggtcctctcc ccccg
//
LOCUS       DATMSX010000502         6537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149409,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000502 DATMSX010000000
VERSION     DATMSX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149409"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VN041_16115"
     CDS             <1..1679
                     /locus_tag="VN041_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWU30606.1"
                     /translation="ALGAAIAAAERARTSFAFSSAVRFGELALELWDQVPDAEAVAGR
                     SHLELLKGLGSLLRNAGHDERAIAVVNLALDEVDDTTPPIVRVKLLRDKALYVQNSGR
                     PGAAGLVRQALAAMDDGVEDERLRAVLLNLLAGRLMVEGDLAAAVDAGEQALRIGREG
                     GFENVVSVAANLIASSRLHLGEVDRGLELYELSWEHARDTDAKLRYWVNHSDSLSTLG
                     RYREALSAAERGVEHAKELGLERSSGSILTENMVEPLLELGEVDRAEELLSKDLTLRS
                     FRIFRVYTTASRIRALIWRGRIDEASALLEEWRPTFLAASEVERQVWYSIIDVEMAIG
                     LASGRLADAAQALERMLDHETPRLAHSARRLLDGALIVAGMRATGDAARASALAERIR
                     EAWSEHHAYFEDWGVLVEGLLNGDAVSLREAIAVADRIDGPVMFRSALRLELARALVA
                     VQGDRGEAATALGEAARIAERTGHARLRRETREFAEASRLGASSARGDGSELTEREQQ
                     VLELIAEGLSNRQIAERLFISIKTVSVHVSAVLRKLGASSRTEAAARHRA"
     gene            1790..1999
                     /locus_tag="VN041_16120"
     CDS             1790..1999
                     /locus_tag="VN041_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWU30607.1"
                     /translation="MTTTEFQVTGMTCGHCEMSVREEVAKIAGVDSIDVSAQTGRLVV
                     SSASAVDSDAVIAAVDEAGYRAVAA"
     gene            2057..4300
                     /locus_tag="VN041_16125"
     CDS             2057..4300
                     /locus_tag="VN041_16125"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWU30608.1"
                     /translation="MTIAEPSRIDLEIGGMTCASCAMRIEKKLNRLDGVTATVNYATE
                     KAQVVVDGPLDPALLITTVENAGYTASVPAPPADADGAGAEPDAEGPELRALRHRLIG
                     SVVLSVPVILLAMIPALQFTYWQWASLALAAPVIVWAAWPFHRAAWVNLRHGAATMDT
                     LISLGTSAAFLWSLYALFLGTAGMPGMTHGFSFALERTDGAGSIYLEVASGVTMFVLA
                     GRYFEKRAKRQAGAALRALMELGAKDVAVLRGGAEVRIPIAELRVGDEFVVRPGEKIA
                     TDGVVVSGTSAVDQSMITGESVPVEVSAGDAVTGATVNAGGRLVVRATRVGADTQLAQ
                     MARLVEDAQTGKAEVQRLADRISGIFVPIVIGIALVTLAAWLLLGFPASMAFTAAVAV
                     LIIACPCALGLATPTALLVGTGRGAQLGILIKGPEVLESTRRVDTIVLDKTGTVTEGR
                     MSVVSVVPEQGTDAAELLRLAGAIEHASEHPIARAIASAATADGAVVELPPVESFQNH
                     EGRGVTGVVEGRAMLVGRLSLLEDWSVHPSAELLSAKADAESAGQTAILVAWDGAPRG
                     MIVVADEVKATSAEAVAQLKALGLTPVLLTGDNQAAARHIAAQVGIDEVIAEVLPVDK
                     VAVVARLQAEGRVVAMVGDGVNDAPALAQADLGMAMGTGTDAAIQASDITLVRGDLRA
                     ASDAIRLSRRTLGTIKGNLFWAFAYNTAAIPLAALGLLNPMLAGAAMAFSSVFVVGNS
                     LRLRSFR"
     gene            complement(4376..4975)
                     /locus_tag="VN041_16130"
     CDS             complement(4376..4975)
                     /locus_tag="VN041_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWU30609.1"
                     /translation="MRIRTLTISTITLATALTLAGCSAPSDMGGMDHGSSSTSPAPSG
                     PSGSTAAAQADIDFAMNMIAHHQQAVEMSDIVLGKDGTAPQVTELAERIKNAQQPEID
                     TMREWLTGWGQDPDMGGMDHGGGMMSPDDLAALEDADGTTASRLFLEQMIMHHEGAVE
                     MARKELAAGKDGDALDLAQRVVDDQTAEITEMRKMLNTL"
     gene            complement(5043..5462)
                     /locus_tag="VN041_16135"
     CDS             complement(5043..5462)
                     /locus_tag="VN041_16135"
                     /inference="COORDINATES: protein motif:HMM:NF040012.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6153 family protein"
                     /protein_id="HWU30610.1"
                     /translation="MQRSTGMPLLLAIALGVVLGLLGMHTFSADPVGHGPGLVSHAIE
                     ADDAAHDSPLSAATHGEPGSPAQGVSSAAQPEPSHSGMVMTCVLALLAGLLLVIRPSL
                     AQRVIRRIRSAVRSRASASRTAPRRPPSHIALCISRT"
     gene            5677..>6537
                     /locus_tag="VN041_16140"
     CDS             5677..>6537
                     /locus_tag="VN041_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWU30611.1"
                     /translation="MAENSSGHGAHAGHAGHGAHAEHDAHAGHDVHPEHGDHSGHAGH
                     GDHSGHGGHAGHGDHVGQFRRLFWINLLIAVPVVASSGMFAMILGYDVPGWARWIAPI
                     LGTVMYVWGGRPFLTGAVSELRSRKPGMMLLIGLAITVAFLASWGASLGILHHELEFW
                     WELALLIVIMLLGHWLEMRSLAQTTSALDSLAALLPDEAERVDGDDITVVSPADLRVG
                     DIVRVRPGGSIPADGVIVDGRADMDESMLTGESRTVSRTTGDSVTAGTVATDSGLRVE
                     VTATGDDTALA"
BASE COUNT          927 a         2234 c         2371 g         1005 t
ORIGIN      
        1 gcgcactcgg cgcggccatc gccgcagccg agcgggcgcg cacgagtttc gcgttctcga
       61 gcgcggtccg cttcggcgag ctcgcgctgg agctgtggga ccaggtgccg gatgccgagg
      121 cggtcgccgg ccggtcgcac ctcgaactgc tcaagggtct cggatccctg ctgcgcaacg
      181 caggacacga cgagcgcgcg atcgcggtcg tgaatctcgc gctcgacgag gtcgacgaca
      241 ccacgccgcc gatcgtgcgc gtgaagcttc tccgcgacaa ggcgctgtac gtgcagaaca
      301 gcggacgtcc cggcgccgcc gggctcgtgc gccaggcgct ggccgcgatg gacgacggag
      361 tcgaggacga gcggcttcgc gcggtgctgc tgaacctgct cgcggggcgg ctcatggtcg
      421 agggggatct ggcggcggcg gtggatgccg gcgagcaggc gctgcggatc gggcgcgaag
      481 gcggcttcga gaacgtggtc tcggttgcgg cgaacctgat cgccagcagc cggttgcacc
      541 tcggtgaggt cgatcgagga ctggaactgt acgagctgtc gtgggagcac gcccgcgaca
      601 cggacgccaa gctgcgctac tgggtcaatc actccgattc gctgagcacg ctgggccgct
      661 accgcgaggc gctgagcgcc gcggaacgcg gcgtcgagca cgccaaggag ctcggactgg
      721 agcgctcgag cggatcgatc ctcaccgaga acatggtcga gccgctgctc gaactgggcg
      781 aggtcgatcg tgccgaggag ctgctgtcga aggatctcac gctgcgcagc ttccgcatct
      841 tccgggtgta caccacggca tcccgcatcc gcgcgctgat ctggcgcggc cggatcgacg
      901 aggcgagcgc gctcctggag gagtggcgtc cgaccttcct ggcggcgtcc gaggtggagc
      961 gtcaggtctg gtactcgatc atcgacgtcg agatggcgat cggcctcgcc tcgggacggc
     1021 tcgccgacgc cgcgcaggct ctcgagcgga tgctggacca cgagaccccg cgcctcgcgc
     1081 acagcgcccg gcggctgctc gacggggcgc tcatcgtggc gggtatgcgc gccacgggtg
     1141 acgctgcccg agccagtgcc ctcgccgagc ggatccgcga ggcgtggtcc gagcaccatg
     1201 cgtacttcga ggactgggga gtgctggtcg aggggctgct gaatggcgac gccgtctcac
     1261 tgcgagaggc gatcgcggtg gcggatcgca tcgacgggcc cgtgatgttc cgctcggcac
     1321 tgcggctcga gctggcgcgg gctctcgtcg ccgtgcaggg cgatcggggt gaggccgcca
     1381 cggcattggg cgaggcggcg aggatcgccg agcgcaccgg gcatgcccgg ctgcgacggg
     1441 agacgaggga gttcgcggag gccagcaggc tgggcgcctc gtcggcccgg ggcgacggat
     1501 cggagctcac cgagcgggag cagcaggtgc tcgagctgat cgccgagggc ctgagcaacc
     1561 ggcagatcgc cgagcgcctg ttcatcagta tcaagaccgt gagcgtgcac gtctccgcag
     1621 tgctccgcaa gctcggtgca tccagccgca ccgaggccgc ggcccgccac cgcgcctgag
     1681 cgcctcgctt ctgaggcttc tcacgatctc gggaataccc cctgggggta tttggtagta
     1741 ctctgatgga agtaccctgc gggggtactc gagacacaga ggagacgcca tgaccaccac
     1801 cgagttccag gtgaccggaa tgacctgcgg gcactgcgag atgtccgtcc gcgaagaggt
     1861 cgcgaagatc gccggggtcg acagcatcga cgtgagcgcc cagaccggcc gtctggtcgt
     1921 cagctcggca tccgccgtcg actccgacgc cgtgatcgcg gccgtcgacg aggccggcta
     1981 ccgcgccgtc gccgcctgac gcaccttccg cacgacaccg attccgccga tccagcctga
     2041 acctgaggag catgacatga ccatcgcaga gccgtcccgc atcgatctcg agatcggcgg
     2101 aatgacctgt gcgtcctgtg ccatgcgcat cgagaagaag ctgaacaggc tcgacggcgt
     2161 gaccgccacc gtcaactacg cgaccgagaa ggcgcaggtc gtggtcgacg gaccgctcga
     2221 cccggccctg ctgatcacca ccgtcgagaa cgccggctac acggcatccg tgcccgcgcc
     2281 gcccgccgat gcggacggcg cgggcgccga accggacgcg gagggtcccg agctgcgcgc
     2341 gctgcgacac cgcctgatcg gatcggtcgt gctgagcgtg ccggtgatcc tgctcgcgat
     2401 gatccccgcg ctgcagttca cgtactggca gtgggcgtcg ctggcgctcg cggcaccggt
     2461 gatcgtgtgg gcggcctggc cgttccaccg agccgcatgg gtgaacctgc gccacggcgc
     2521 ggccaccatg gacacgctga tcagcctcgg cacgtcggcc gcgttcctgt ggtcgctgta
     2581 cgcactgttc ctcggcaccg ccgggatgcc cggcatgacg cacgggttct cgttcgcact
     2641 cgagcgcacc gacggtgccg ggagcatcta cctcgaggtc gcctcgggcg tgacgatgtt
     2701 cgtgctcgcc gggcgctact tcgagaagcg cgccaagcgc caggcggggg cggcgctgcg
     2761 cgcgctgatg gagctcgggg cgaaggatgt cgcggtgctc cggggtggcg ccgaggtgcg
     2821 catcccgatc gccgagcttc gcgtcggcga cgagttcgtc gtccggcccg gcgagaagat
     2881 cgcgacggac ggcgtggtcg tctccggcac gtcggcggtg gaccagtcga tgatcaccgg
     2941 cgagtccgtc ccggtcgagg tgtcggcggg ggatgccgtg accggcgcca ccgtgaacgc
     3001 cggcgggcgc ctcgtcgtgc gagccacccg ggtcggcgcc gacacgcagc tcgcccagat
     3061 ggcgcgcctc gtcgaggacg cgcagaccgg caaggccgag gtgcagcgac tggccgaccg
     3121 gatctcgggg atcttcgtgc ccatcgtcat cggcatcgcc ctggtgacgc tcgccgcatg
     3181 gctgctgctg ggcttccccg catccatggc cttcaccgcc gcggtcgcgg tgctcatcat
     3241 cgcctgcccg tgcgccctgg gcctcgcgac ccccacggcg ctgctggtcg ggacgggtcg
     3301 cggcgcgcag ctgggcatcc tgatcaaggg cccggaggtg ctcgagtcca cccgccgcgt
     3361 cgacacgatc gtcctcgaca agaccggcac ggtcaccgag ggccggatgt ccgtcgtctc
     3421 cgtcgtgccc gagcagggca cggatgccgc ggagctgctg cggctggccg gagcgatcga
     3481 gcacgccagc gagcacccga tcgccagggc catcgcatcc gctgcgaccg cggacggggc
     3541 ggtcgtcgag ctcccgcccg tcgagtcgtt ccagaaccac gagggtcgcg gcgtcacggg
     3601 cgtcgtcgaa ggacgcgcca tgctcgtcgg gcgtctgtcg ctgctggagg actggtccgt
     3661 gcatccgtcc gccgaactgc tgtcggcgaa ggcggatgcc gagtccgcag ggcagaccgc
     3721 gatcctggtc gcctgggacg gtgcgccgcg cgggatgatc gtggtcgccg acgaggtcaa
     3781 ggcgaccagc gccgaggccg tcgcgcagct gaaggccctc gggttgaccc ccgtgctgct
     3841 gaccggtgac aaccaggctg ccgcccggca catcgccgcg caggtcggca tcgacgaggt
     3901 gatcgccgag gtgctgccgg tggacaaggt cgccgtcgtg gcgcggctgc aggccgaggg
     3961 ccgcgtcgtc gcgatggtcg gcgacggcgt caacgacgct cccgccctcg cgcaggccga
     4021 tctgggcatg gcgatgggca cgggcacgga tgcggcgatc caggcatccg acatcaccct
     4081 cgtgcgcggg gatctgcgcg cggcatccga tgccatccgc ctctcgcgcc gcacgctcgg
     4141 caccatcaag ggcaacctgt tctgggcgtt cgcgtacaac accgcggcga tcccgctcgc
     4201 ggcgctgggc ctgttgaacc cgatgctggc gggagccgcg atggccttct cgagcgtgtt
     4261 cgtcgtgggc aacagcctgc gtctgcgctc cttccgctga acggcgtcac cgagaggacg
     4321 acgatgcaaa ggggcgggcc gcgatcgcga cccgcccctt tcgtgcgccc gagagtcaga
     4381 gcgtgttgag catcttccgc atctcggtga tctcggccgt ctgatcatcg accacgcgct
     4441 gcgcgaggtc cagggcgtcg ccgtccttcc cggcagccag ttccttccgg gccatctcga
     4501 cggcaccttc gtggtgcatg atcatctgct ccaggaacag tcgagaggcg gtggtgccgt
     4561 cggcgtcctc gagtgccgcg aggtcgtcgg gcgacatcat cccgccgccg tggtccattc
     4621 cgcccatgtc ggggtcctgc ccccacccgg tcagccattc ccgcatggtg tcgatctcgg
     4681 gctgctgggc gttcttgatc cgttcggcga gttcggtcac ctgcggggcg gtgccgtcct
     4741 tcccgagcac gatgtcggac atctcgaccg cctgctggtg atgggcgatc atgttcatcg
     4801 cgaagtcgat gtcggcctgg gccgcggcgg tggagccgct cggaccgctc ggtgcgggtg
     4861 aggtgctcga ggagccgtga tccatcccgc ccatgtcgga cggagccgag catccggcca
     4921 gcgtgagggc cgtcgcgagc gtgatggtgc tgatggtgag tgtgcggatt cgcatctgag
     4981 tctccttggt gtctgtcgtc tgggtcgtgg gggtgatgcc gcgcgcggcg tccatcccgg
     5041 actcaggtcc gactgatgca gagcgcgatg tgcgagggcg gacggcgtgg cgcggtccgg
     5101 gaagcggatg ctctgctgcg gaccgccgag cggatgcggc ggatcacgcg ctgcgcgagc
     5161 gaggggcgga tcacgagcag cagacctgcg agcagggcca gcacgcacgt catgaccatg
     5221 ccggagtgcg agggttcggg ctgtgcggcg gacgagacgc cctgtgcggg ggatccgggc
     5281 tcgccgtgtg tcgccgcgct caggggtgag tcgtgggcgg cgtcgtcggc ctcgatggcg
     5341 tggctgacga ggcccggccc gtgtcccacc gggtcggcgc tgaaggtgtg catcccgagc
     5401 aggccgagga ccacgccgag tgcgatcgcc agcagcaggg gcatgccggt actgcgctgc
     5461 atggtccgcc tccccttctc gacatcttcg ggactcttgt cgggaatacc ccctgggggt
     5521 atcttagttg gaagacatca gaagccggta cccccggcac cggatgccga ggaggccccg
     5581 atggacccgc acaaccacgc cgagcatatg cgtcacgcgc ccgttcggga ggagaactcc
     5641 tcgatgggag gagcctccga gcacgagact cctcccatgg ctgagaactc ctccggccat
     5701 ggtgcgcatg cggggcatgc ggggcatggc gcgcacgcag agcacgacgc gcacgcgggc
     5761 cacgacgttc acccagagca cggcgaccac tccggccacg ccggccacgg cgaccactcc
     5821 ggtcacgggg gccacgccgg ccacggcgac cacgtcggcc agttccgccg gctcttctgg
     5881 atcaatctgc tgatcgccgt cccggtggtc gcctccagcg gaatgttcgc gatgatcctc
     5941 ggctacgacg ttcccggctg ggcgcgctgg atcgctccga tcctcggcac ggtcatgtac
     6001 gtctggggcg ggcggccgtt cctcaccggc gccgtctccg agctcaggtc gcgcaagccc
     6061 ggcatgatgc tgctgatcgg cctggcgatc acggtggcgt tcctcgcctc ctggggcgcg
     6121 agcctgggca tcctgcacca tgagctcgag ttctggtggg agctcgccct gctgatcgtg
     6181 atcatgctgc tcgggcactg gctggagatg cgctcgctcg cgcagaccac ctcggccctc
     6241 gactcgctcg cggccctgct gcccgacgag gccgagcggg tggacggcga cgacatcacc
     6301 gtcgtcagtc ccgccgatct gcgggtgggt gacatcgtgc gggtgcgccc cggcggcagc
     6361 atcccggccg acggcgtgat cgtcgacggt cgcgccgaca tggacgagtc catgctcacc
     6421 ggggagtccc gcacggtcag ccgcaccacc ggcgactccg tcaccgccgg caccgtcgcg
     6481 accgattccg ggctgcgcgt cgaggtcacc gccaccggcg acgacaccgc gctcgcc
//
LOCUS       DATMSX010000503        16164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_102437,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000503 DATMSX010000000
VERSION     DATMSX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102437"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VN041_16145"
     CDS             <1..120
                     /locus_tag="VN041_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWU30612.1"
                     /translation="VIDALGAGERRLRVLAADRSRAFSWASSAERLWALHAEL"
     gene            264..2255
                     /locus_tag="VN041_16150"
     CDS             264..2255
                     /locus_tag="VN041_16150"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HWU30613.1"
                     /translation="MATLLAIFALVLGAAVPANAASSPVSTLAGHPVKTSLAGFSAGN
                     IISDAVFTARSTMTEAQIQAFFNSKVKTCRGGIDENGRPIVCLKDFKQTTVNRPADAY
                     CKGYTGAANESAARIVYRVSQSCGINPQVLIVMLQKEQSLVTHTWPSAWRYDKALGQA
                     CPDTAPCDPKFVGFFHQVYGAARQMQLYMEGRYFTWYAPGKTWNILYNPNKACGSSPV
                     YVANKATSALYYYTPYQPNAAAIKAGYGTGDSCSSYGNRNFYNYFTDWFGSTQAQLPK
                     TFTMGSVAVEGKAIVGQTLRASVTVSPQPASLGFQWYRNGTMVSGATASAYSLTNADR
                     GAAIKVSVTAKSSGYVDGTATSASANIAAVSVERISGANRVETAIAVSRAAWPSGTGT
                     VMLATGDDFPDALSAAAAAGRAAGSLLLTPARALPAEVIAEIKRLGPSRIVLVGGEGV
                     LSSNVAAQAAAAAPGAKVERLAGADRYATSRAVAEASGASTQLLIATGNDFPDAISAA
                     AAGNSIGAPVLLVDGVRSQADAATIATIKKIGAKTATIVGGEGVISRGISVQLASIGL
                     STSRLSGADRFSTNSAVAQKFFPTTALKVLLASGQDFPDALSASVLAGRWKVPLLLTT
                     RSCVVVSAADFMQRAGAESVAVIGGAGVLDDRVVALQRC"
     gene            2409..3314
                     /locus_tag="VN041_16155"
     CDS             2409..3314
                     /locus_tag="VN041_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30614.1"
                     /translation="MSRTEFDIPGRSHGLIDVVRWRYLLRLLVRTGVTTRYRNSVLGW
                     AWSYVRPAAQFLVFWIVLGIFMNLDRVPNYAVYLFSGIVVINLFSEGFKNATTSIVGN
                     APLVRKVFLPRQLFAVSAVLVSFVHFLPQVALLLFVCLLMGWAAHISVLSILAVLAGM
                     AIVMVFSLGLGLFFGAVNVRFRDAENIVELMLLLATWASPVLYAWTMVETAIVEKLGW
                     PHWIVELYLLNPITQGVELFHYAFWRPASQEIVNSAGYAGDALTAIPPDLAVNTLWTA
                     LIAIGTLLIGQFVFRRLEGKFAQDL"
     gene            3311..4045
                     /locus_tag="VN041_16160"
     CDS             3311..4045
                     /locus_tag="VN041_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30615.1"
                     /translation="MSATASRPSIIINGVRKTFTLNHAFSLKDTVVSWVRRRKLTSTF
                     EALKGVDLVIGEGESVAILGMNGSGKSTLLKLISGVMEPDAGEVLTRGRVAGLIEVGA
                     GFHPELSGRENVFLNAAILGMKKHEIEALYDEIVAFSEIEEFIDQEVKHYSSGMFLRL
                     AFAVAIHVHLDVLLMDEILSVADVPFREKCRRKFEELIAQGKTLIVVSHDMEMVRELC
                     TRGVVISKGEVIYDGELEGAIALVDA"
     gene            4042..5934
                     /locus_tag="VN041_16165"
     CDS             4042..5934
                     /locus_tag="VN041_16165"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HWU30616.1"
                     /translation="MSADWLAQTPAFLATLALLILPGMPMALLLRLRGVLVPGAAIVG
                     SLASIGAGSVVAPLLGMRWSPLPVLIVALTITAIAAVLWAIKRDKALQLRGADDRWVW
                     AGIGAAALGWALIVILGVSSASHPSQLFDGIFHLDAVEFILQHGDASPFHMTMVVPGD
                     ASALYPTLWHALVALVVPFSGAVVPATNVVTVVVVAVVWPVAIAVLTSVLFPSARRAA
                     VWAPLAAFGFSVFPLGFLNWGVLYPNLIGMLQVPLTLGFVLLAARPRVPWPQRFGLVL
                     LAVASAGATAMGHPSAMLGAVVLLVPWAAWHGWRVARGHGARGRVLFTAAAVLVLVAL
                     IGVWRSLNVSTGEWLPNMTMAQGIGEVAFLSPVGRTAGLLIGPLAAIGIWRTARSRRW
                     WILWSHAAAAGFYLAAVWLPILSIRTAIVGIWYDDATRVASLLGVLGLPLAGLGAAVV
                     LEWLRHLRAAGRRRRAAAVLTAAILLGATHLVAVWNDISFMRNTSFRFDSASQGLTPD
                     EAALFEKADRILESDSRVIGDPLTGASLLYAYTGHDVVFPHVSGRYGPDAMLLARSMN
                     TGSTEVCEAVRRLGVTYAVDFGGKELFENYYTTFDGLHDLKDSKILTEVARVGDAALF
                     EVTGCS"
     gene            complement(5947..6306)
                     /locus_tag="VN041_16170"
     CDS             complement(5947..6306)
                     /locus_tag="VN041_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HWU30617.1"
                     /translation="MWIQFLLIIAVVVLGGVVMRRTGADSHLAIRRLLMMLFVLAAVL
                     SILFPQWLTWVATLIGVGRGTDLLLYALVVMFLAFVYTQFRRNAQLERRLTLLSRRIA
                     LMDAEVHATDDEIETGS"
     gene            complement(6309..6980)
                     /locus_tag="VN041_16175"
     CDS             complement(6309..6980)
                     /locus_tag="VN041_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU30618.1"
                     /translation="MIDESAWVVIPLYNEGTVIGGVIRDLLQHFSHVVCVDDGSSDDS
                     AAAARAAGALLVQHPINLGQGAALQTGFEFALGQPKCEYVVTFDADGQHRIEDALQML
                     EVARRDDIAIVFGSRFLDKRTNPGLIKRVVLKTAVWVTNMTTGMHLTDAHNGLRVIRR
                     DALSQVDLKQDRMAHATEIVIALGSTGLPWREHPVELLYTDYSKGKGQSVLNSINILV
                     DLVVR"
     gene            7163..7774
                     /locus_tag="VN041_16180"
     CDS             7163..7774
                     /locus_tag="VN041_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWU30619.1"
                     /translation="MATSASPRIVDSADVSPDASIGDGSSIWHLAQVREGVQMGENCI
                     VGRGAYIGTGVEMGDNCKVQNYALVYEPAKLADGVFIGPAVVLTNDTYPRAINADGTI
                     KSAHDWEPVGVTIERGAAIGARATCVAPVTIGAWATVAAGAVVVKDVPAYALVAGVPA
                     RRIGWVGESGVPLVAGDDETTWICPTTGDRYFETNGTLTKEAA"
     gene            7771..8868
                     /locus_tag="VN041_16185"
     CDS             7771..8868
                     /locus_tag="VN041_16185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWU30620.1"
                     /translation="MSEFIPPAKPIIGDEEREAVDRVLRSGMVAQGPEVAAFEQEFAA
                     HFVPGRPAVAVNSGTAGLHLGLLAAGVGAGDEVIVPSFTFAATGNSVALTGGTPVFVD
                     IEPETFTLDPEAVAAAITPRTKGILPVHLYGHPAHMRELEKLAADRGVALYEDAAQAH
                     GASLDGRPVGSFGEFAMFSLYPTKNMTSGEGGMVTTATAEIARRVKLLRNQGMERQYE
                     NEVIGFNARMTDIHAAIGRVQLTKVDAWTKTRQQNAAFLDANLRGVVAPPVAEGAVHV
                     YHQYTIRVPEDRDGFVKALKEEHQVGAGVYYPIPNHRLPSLAPYAPGLDLPETERAAR
                     EVVSLPVHPSLSQGDLDRIVAAVNAVAGAGA"
     gene            8868..9854
                     /locus_tag="VN041_16190"
     CDS             8868..9854
                     /locus_tag="VN041_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWU30621.1"
                     /translation="MAVLRAGLLGVGMMGRHHARVLRELDGVDLVGIADPGGDPHGVA
                     GNLEVLPDIDALIAVGIDIAVVAVPTRFHEDAALKLAAAGVHTLVEKPIAHSLEAGQR
                     MVDAFKAGGLVGAVGHIERFNPALQEMRRRIESGELGAVYQVATRRQGPFPSRIADVG
                     VAKDLASHDVDLTAWVVQSDYDHVFAQTAFKSGREHEDMIVITGRTKSGVIVSNTVNW
                     LSPMKERVTVVTGEKGAFVADTSTGDLTFYANGTIPLEWESVSTFRGVSEGDVTRYAF
                     AKREPLKVEHEAFRDAVLGQSTDVVTMEQGQQTLAVVEAALESARTAAAHRL"
     gene            9895..10947
                     /gene="wecB"
                     /locus_tag="VN041_16195"
     CDS             9895..10947
                     /gene="wecB"
                     /locus_tag="VN041_16195"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWU30622.1"
                     /translation="MKILSVVGARPQFVKLAPVAHAMVGRAEHIVVHTGQHYDELMSD
                     VFFRDLGIPAPDVNLAIGSGNHGAQTGAMLEGIEEVLLERRPDWVLVYGDTNSTLAAA
                     VAAIKLHIPVAHLEAGLRSFNRRMPEEHNRVLTDHASDLLLAPTAVAAAHLSNEGLAE
                     KTVVVGDVMTDVLTGVRDRVAGDPLPDLVSEGDYYVATIHRPDNTDDADHLRGIVDGL
                     AALDKPVLLLAHPRLVHTAERLGVALEGGSLQTRAPLAYPQLVRAVMGAAGVITDSGG
                     LQKEAFLLRTPATTVRPETEWVETVDLGWNVLVAPERDAIVEAVHRPAPHATEETPYG
                     DGSAAVRVVDELLARS"
     gene            complement(10981..12138)
                     /locus_tag="VN041_16200"
     CDS             complement(10981..12138)
                     /locus_tag="VN041_16200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30623.1"
                     /translation="MTAAAAAHARPRIAILSPAPLARSPRALKQVRALRGDHDIVTAS
                     RGDSPFPEIEHVRLPDREQRWGALGRILYLMVLALHLYPLITRLSAQDHAIRAALGDR
                     DWDLVIAHDVQTATVAETLRSRRGVLVDLHEYFPRQNEHSLAWRLLIAPYFRWILSGP
                     VHRAAATTTVSSGIVEEYRREHDLDPTLIINASPYQERQAAPVHAPLRLVHSGVAAPH
                     RHVEILIDAVKASEADVTLDLYLVGADSDYGRGLRERASADARIRVHEPVPYDELIAT
                     LADHDLGLHVLPPINFNHLHALPNKLFDYVQARLGVVIGPSPEMARIVREAGVGVVAD
                     DFTADSLHAAIDALTPEAVAEYKAASERSARDLSSERQTEILLNLVSRLLD"
     gene            complement(12135..13301)
                     /locus_tag="VN041_16205"
     CDS             complement(12135..13301)
                     /locus_tag="VN041_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU30624.1"
                     /translation="MQGSARKKILVFTFTPINREPRALKQIELLCDDHDVTTAGFGPA
                     PVEGVPHIELESLPTSHGLLAIRGMYSLIILLRQYRLLSKVLPRNASALARLTPHEWD
                     LIIAHDVSTVPVARKLRSRFGVLVDLHEYATRQGENSLRWRLVEGGYFRWILKRHVRR
                     AAAVSTVGQGIVDEYRREFGIECDLVVNATPYRDIEPSKTPEPIRMVHSGAVAATRKL
                     EIMIEAVKLTRTPVTLDLYLVGAEDAYFASLRKMAEGDPRIRFRDPVPYDQLVTTLSQ
                     YDIGLAMIAPSTFNLLWCLPNKFFDYIQARLAIITGPSPEMARITREHDLGAVTEEFT
                     GESLAALLDRIDAAKVDAWKASAHRQARELSSEVQVKVWRRMVDRIFSRSDASA"
     gene            complement(13324..14088)
                     /locus_tag="VN041_16210"
     CDS             complement(13324..14088)
                     /locus_tag="VN041_16210"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HWU30625.1"
                     /translation="MTLFPRVIPVLLVSDGYLVKPVKFSGDTYIGDPINAVRIFNEKQ
                     VDELLICDIDASAKGTGVNYTLIEEIASEAFMPVGYGGGVASADEARRITGIGIEKVV
                     LNTAAFARPEVITEVSEALGSSSTVVSVDAKRRLTGGWDTYTARASRKTGLTPAAAAA
                     QAQQYGAGEVMISSIDRESSRSGYDLKLIQSVADAVTIPIVALGGAGSLADFAPALAA
                     GASAVAAGTMFVMTGKHRAVLITYPPAADIRSLRPS"
     gene            complement(14085..14690)
                     /gene="hisH"
                     /locus_tag="VN041_16215"
     CDS             complement(14085..14690)
                     /gene="hisH"
                     /locus_tag="VN041_16215"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWU30626.1"
                     /translation="MTVGIVDYGLGNLGSVRNMLKRVGAQSVSVRTADEIAEVDKVLL
                     PGVGAFDTGVTRLAEAGLIDALRDFAGTGRPLLGICLGMQLLLDGSDEGVKSGLGLIP
                     GRSVRFDEKLGIRVPHMGWNLANPAREDPLVAELPEESRFYFVHSYHVVPERAEDTLA
                     ITDYGIPFSSMVRTGNVMGAQFHPEKSHTFGMTIMRNFAAL"
     gene            complement(14687..15847)
                     /locus_tag="VN041_16220"
     CDS             complement(14687..15847)
                     /locus_tag="VN041_16220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HWU30627.1"
                     /translation="MTGGTRPYQRCTRTIMDTTDPNITFDAEGVSNYALDYDQYMGKV
                     VDRALSGEAEPELQALVARIKESGRGKPYDCIIGVSGGVDSTYLLMRAVELGLRPLAV
                     HFDSGWNSELAVGNIQNLINATGVDLYTHVVDWREMKDLQLSFFKASVANCDIPTDHA
                     FGWVAFDQAKKYGIKYILSGSNYASESILPSAWGYSSDDAKHLKAIQKRFGSVKLKTY
                     PIIGRFKRHIWYPYVRGIRTERPLNLMPYVYKDAKAAITERVGWRDYGGKHYESVFTR
                     YFQGYYLPHKFGFDKRLAHYSSLIVSHQLGRDEALTLMETENYPEDLRAQDHEFIAKK
                     LGVSTAELERIFDAPPVDYTAYPNSEKSWNLLLSVLRVPARIMKSLGMKKAA"
     gene            15935..>16164
                     /locus_tag="VN041_16225"
     CDS             15935..>16164
                     /locus_tag="VN041_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30628.1"
                     /translation="MSESQQAHSESRPQILSISFSPLDRDARVLRQIAVLSEFGDVTT
                     VGFGPAPAGAARHIEVPAGLPSLPQTPAGVLKL"
BASE COUNT         2570 a         5294 c         5476 g         2824 t
ORIGIN      
        1 gtcatcgacg cgctcggcgc gggggagcgg cgcctgcgcg tgctcgccgc ggaccggtcg
       61 agggccttct catgggcgag ctccgcggag cgcctctggg cgctgcacgc ggagctctga
      121 gagtctcctc tcaagttcgc gacacgccgg cgcggatgcg ccaatattcc gccccactgg
      181 catcatccgt caacttcagt cacactattc acatgcatgc cagcggtttt cccggtccgc
      241 gctcgttccg aagtgcccga tgggtggcga ctcttctcgc catcttcgcg cttgtgctcg
      301 gggcggcggt gccggcgaac gccgcatcca gccccgtcag caccctcgca ggccaccccg
      361 tgaagacctc gctcgcaggc ttctccgccg gcaacatcat cagcgatgcg gtgttcaccg
      421 ccagatccac gatgactgag gctcagatcc aggccttctt caactccaag gtgaagacgt
      481 gccgaggcgg catcgatgag aacggacggc cgatcgtctg cctcaaggac ttcaagcaga
      541 ccaccgtgaa ccgtcccgcc gacgcgtact gcaaggggta caccggcgcc gccaacgagt
      601 cggccgcgcg catcgtctac cgcgtctcgc agtcgtgcgg aatcaacccg caggtgctca
      661 tcgtcatgct ccagaaggag cagagcctgg tcacgcacac ctggcccagc gcgtggcggt
      721 acgacaaggc gctcgggcag gcctgccccg acaccgcgcc gtgcgacccg aagttcgtcg
      781 gcttcttcca ccaggtgtac ggggccgcgc gccagatgca gctgtacatg gaaggccgct
      841 acttcacctg gtacgcgccg ggcaagacgt ggaacatcct ctacaacccc aacaaggcct
      901 gcgggagctc gccggtctac gttgcgaaca aggcgacgtc agcgctctac tactacacgc
      961 cgtatcagcc gaacgcggcg gcgatcaagg cgggttacgg cacgggtgac tcctgctcca
     1021 gctacgggaa caggaacttc tacaactact tcaccgactg gttcggctcg acgcaggcgc
     1081 agcttcccaa gaccttcacg atgggaagcg tcgctgtcga gggtaaggcg atcgtcgggc
     1141 agacgctgag agcatcggtc acggtatctc cgcagccggc ttccctcgga ttccagtggt
     1201 atcggaacgg aaccatggtg tcgggtgcga ctgcatcggc atactcgctg acaaacgcgg
     1261 acaggggcgc tgcgatcaag gtcagcgtga ccgcgaagag ctccggctat gtggacggga
     1321 cagcgacttc ggcgtcggcg aacattgcgg cagtgagcgt ggagaggatc tccggggcga
     1381 atcgcgtcga gacggcgatc gccgtctcac gagccgcgtg gccgtcaggt actggcacgg
     1441 tcatgctggc caccggtgat gattttcctg acgcgctcag tgcggctgcc gctgcgggta
     1501 gagcggcggg ctcgctactg ctcacccccg cgcgtgcact ccctgctgag gtgatcgccg
     1561 agatcaagcg cctgggcccg agtcgtatcg tgctggtcgg tggagaagga gttctttcgt
     1621 ccaacgtcgc cgcacaggcc gcggccgccg cgccgggcgc gaaggtcgag cggcttgctg
     1681 gggccgaccg ctatgcaacg tcccgagcgg tggcggaggc gagcggagca tccactcagc
     1741 tgctgatcgc aacaggaaac gacttcccgg atgcgatcag cgcggcggcc gccggcaaca
     1801 gcatcggcgc tccggtgctg ctcgtcgacg gcgtgcggtc gcaggctgat gctgcgacga
     1861 tcgccacaat caagaagatc ggtgccaaga cggcgaccat cgtcggcgga gaaggcgtca
     1921 tctcgcgcgg aatcagcgtt cagctcgcat cgatcggtct gtccacgtca cggctgtccg
     1981 gtgctgatcg attctcgact aacagtgcag tcgcccagaa gttcttccca acgacagcgc
     2041 tgaaggtgct tctggcctcc ggccaggact tcccagatgc gctatccgcc tcagtgctgg
     2101 ctggacggtg gaaagtgccc ctgctgctga ccacgagatc gtgcgtggtc gtgagcgcgg
     2161 ccgacttcat gcagagggcg ggagccgagt cggtcgcagt catcggtgga gctggcgtcc
     2221 tcgatgatcg ggtcgtggcg ctgcaacgct gctgatcgct tcgcgtcgct ggtgtggcgc
     2281 ccttggaggg atcgaaaccg ccgcactggt actcccgggc tcaggcaatt gcccgaggca
     2341 tcgcggtaac atgatcagcg acctttcggc tggagccctt cttgacaacc aatgatcaat
     2401 tcgctcttgt gtcgcgcact gaattcgata tccccggccg gagccatggg ctgatcgacg
     2461 tggtgcgctg gcgctatctg ctgcgcctgc tcgtgcgtac cggtgtgacg acccggtacc
     2521 gcaactccgt gttgggctgg gcatggtcgt atgtccgtcc ggccgcgcag ttcctggtct
     2581 tctggatcgt cctcgggatc ttcatgaatc tcgatcgggt tcccaattac gcggtgtatc
     2641 tgttctcggg catcgtcgtc atcaacctgt tctccgaagg attcaagaac gccacgacct
     2701 cgatcgtcgg caacgctcct ctggtgcgca aggtgttctt gcctcgtcag ctgttcgcgg
     2761 tctcggccgt gctcgtgtcg ttcgtgcact tcctgccgca ggtggccctg ctgctgttcg
     2821 tctgcctgct gatgggctgg gccgcgcaca tctccgtgtt gagcatcctc gcggtgctcg
     2881 cgggcatggc gatcgtgatg gtgttttcgc tcggcctggg gctgttcttc ggcgcagtga
     2941 acgtgcggtt ccgcgacgcg gagaacatcg tcgagctcat gctgctgctg gcgacgtggg
     3001 catctccggt gctgtacgcc tggacgatgg tcgagaccgc catcgtggag aagctcggct
     3061 ggccgcactg gatcgtcgag ctctacctgc tgaacccgat cacacaggga gtcgagctgt
     3121 tccattacgc cttctggcgt ccagccagtc aggagatcgt gaactccgcc ggctatgccg
     3181 gcgacgcgct caccgccatc ccgccggatc ttgcggtcaa cactctctgg accgcgctga
     3241 tcgccatcgg aaccctgctg atcggccagt tcgtcttccg ccgccttgag ggaaagttcg
     3301 cccaggatct atgagtgcca cagcaagccg gccgagcatc atcatcaacg gtgtgcggaa
     3361 gacattcacg ctcaatcacg cgttctcgtt gaaggacacc gtcgtctcgt gggtgcgtcg
     3421 ccgcaaactc acctcgacgt tcgaggccct caaaggcgtc gacctcgtga tcggcgaggg
     3481 ggagtccgtc gcgatcctcg ggatgaacgg ctcgggaaaa tccacgctcc tcaagctcat
     3541 ctcgggcgtc atggagcctg acgcaggcga ggtgctcacc cgtggtcgcg tcgccggatt
     3601 gatcgaggtg ggcgcaggat tccaccccga gctctccggt cgcgagaacg tgtttctcaa
     3661 cgcggcgatc ctgggaatga agaagcatga gatcgaagcg ctctacgacg agatcgtcgc
     3721 gttcagcgag atcgaggagt tcatcgatca agaggtcaag cactactcgt ccgggatgtt
     3781 ccttcgcctg gccttcgctg ttgcgatcca cgtccatctg gacgtgctcc tgatggacga
     3841 gattctctct gtcgccgatg tgccgttccg tgagaagtgc cgccggaagt tcgaggagct
     3901 gatcgcgcag ggcaagaccc tgatcgtcgt gagccacgat atggagatgg ttcgcgaact
     3961 ctgcaccagg ggagtggtga tcagcaaggg cgaggtcatc tatgacgggg aactcgaggg
     4021 cgcgatcgcg ctggtggacg cgtgagcgcg gactggctgg cgcagacgcc cgcattcctc
     4081 gcaaccttgg cgctgctgat tctgcccggg atgccgatgg cgctgttgct gcgtcttcga
     4141 ggcgtgctgg tgccgggggc ggcgatcgtc ggctcgctgg ccagcatcgg cgccggctct
     4201 gtcgtcgctc ccttgctggg catgcgctgg tcgccgcttc cggtcctgat cgtggccctg
     4261 acgatcacgg cgatcgctgc ggtgctctgg gcgattaagc gcgacaaggc gctccagctt
     4321 cgcggcgccg acgatcgatg ggtatgggct ggcatcggcg ctgccgcgct cggctgggcg
     4381 ctcatcgtca tcctgggcgt gagcagcgcc tcgcacccgt cgcagctgtt cgacggcata
     4441 ttccacctgg acgccgtcga gttcatcctc cagcacggtg acgcgtcgcc gttccacatg
     4501 accatggtgg tgcccggcga tgcgtccgcg ctgtacccca ccctgtggca tgcgctcgtc
     4561 gcgctcgtcg tgcccttctc gggggcggtg gtccctgcga ccaacgtggt cactgtcgtc
     4621 gtggtggccg tcgtatggcc cgtcgcaatc gcagtgctga caagcgtgct cttcccgtcg
     4681 gcgcgccgag ccgctgtctg ggcgcctctc gccgcgtttg gcttcagcgt gtttcccctc
     4741 ggattcctga actggggcgt gctctatccc aacctgatcg ggatgctgca ggtccctttg
     4801 acgctcggct tcgtgctcct ggcggcgcgt ccccgtgttc cgtggccgca gcgtttcggg
     4861 ctcgtgctgc tcgcggtggc ctcggcaggg gcgacggcca tggggcaccc gtcggcgatg
     4921 ctgggggcag tcgtgctcct agtgccatgg gcagcctggc acggctggcg agtcgcccga
     4981 ggtcacgggg cacgtggtcg tgtgctcttc acagcagccg cggtactcgt gctggtcgcg
     5041 ctcatcggcg tgtggcgatc gctgaacgtc agcacgggcg aatggctgcc gaacatgacc
     5101 atggcacagg gaatcggcga ggtggccttc ttgagcccgg tcgggcgtac ggcgggtcta
     5161 ctgatcggcc cgcttgccgc gatcgggatc tggcgaactg cgcgttcgag gcgatggtgg
     5221 atcctctgga gccacgcagc ggccgccggg ttctacctcg ccgccgtatg gcttccgatc
     5281 ttgtccatcc gcactgcgat cgtcggcatc tggtacgacg atgcgacgcg cgtcgcatcg
     5341 ttgctcggcg tgctgggcct cccgctcgcc ggactcggcg ctgcggtggt gctcgaatgg
     5401 ctccggcacc tgcgcgcggc tggacgtcgt cgtcgggccg cagccgtcct gaccgcggcg
     5461 atcctgctgg gtgccactca cctggtcgcc gtgtggaacg acatttcgtt catgcgcaac
     5521 accagcttcc gattcgactc cgcgtcgcag gggctcacgc ccgatgaggc agccctgttc
     5581 gagaaggcgg accgaattct tgaatcggac agcagagtca tcggtgaccc cttgaccgga
     5641 gcgtcgctgc tgtacgccta cacaggtcat gatgtcgtct tcccgcacgt gtcaggtcgc
     5701 tatggcccgg atgcgatgct cctggcccga tcgatgaaca ccggctcaac cgaggtatgc
     5761 gaagcggtgc ggcgcctcgg cgtcacctac gccgtcgact tcggcggcaa ggagctgttc
     5821 gagaactact acacgacgtt cgatggactg cacgacctca aggattccaa gatcctgacc
     5881 gaggtcgcga gagtagggga tgctgcgctg ttcgaggtga ccggctgcag ttagatcccg
     5941 cagaggtcag gacccggtct cgatctcatc gtctgttgcg tgaacctcag catccatcag
     6001 cgcgatcctc cgcgacagaa gcgtgagacg acgctcgagc tgggcgttgc ggcggaactg
     6061 cgtgtacacg aacgccagga acatcactac cagggcatag agcagaaggt cggtgccacg
     6121 cccgacgccg atcagggtcg ccacccacgt gagccactgg gggaacagga tcgacagcac
     6181 ggccgcgagc acgaacagca tcatcagcag gcggcggatt gccaggtggc tgtccgcacc
     6241 ggtgcgtcgc atgaccacac cgccgaggac gaccacggcg atgatcagca ggaactggat
     6301 ccacatggtc acctcaccac caggtcgacg aggatgttga tggagttgag cacgctctgg
     6361 cccttgccct tcgagtagtc cgtgtacagg agctcgaccg gatgctcacg ccatggcagg
     6421 cctgtgctgc cgagtgcgat cacgatctcc gtggcgtgcg ccatccggtc ctgtttgaga
     6481 tcgacctgag acagcgcgtc ccgccggatc acgcgtagcc cgttgtgggc gtccgtcagg
     6541 tgcatcccgg tggtcatgtt cgtcacccag acggctgtct tcagcacgac gcgcttgatc
     6601 aaccccgggt tcgtgcgctt gtcgaggaag cgcgagccga agacgatggc gatgtcatcg
     6661 cgacgtgcga cctcgagcat ctggagtgcg tcttcgatcc gatgctgtcc gtctgcatcg
     6721 aaagtcacga catactcgca tttcggttgg ccaagggcga actcgaagcc ggtctgcagc
     6781 gcggcacctt ggccgagatt gatcggatgc tggaccagca gcgcgcccgc cgcgcgggcc
     6841 gctgcggcgg agtcgtcgga tgagccgtcg tccacgcaca ccacatgcga gaagtgctga
     6901 agcagatcgc ggatgacgcc gccgatgaca gtgccctcgt tgtagagcgg gatcaccacc
     6961 caggcgctct cgtcgatcat cgcgggcgcg tgcacaccgg tgccgtcggg ggacgtggat
     7021 gatggggtca cccccacatt ctcgcaggcc tcgtattgat cagcgactcg ggcgccttcc
     7081 gtgtctgaga tggaggccga tacgggcacg gtaagctgtg agggcgatcc ggcagcatcc
     7141 gagtcggcgc agaaagctga acgtggcaac atctgcttcc ccgcgcatcg tcgattccgc
     7201 cgacgtctcg cccgatgcct ccatcggcga cggctcctcg atctggcacc tcgcgcaggt
     7261 gcgcgagggt gttcagatgg gcgagaactg catcgtcggg cgtggcgcat atatcggtac
     7321 aggtgtcgag atgggcgata actgcaaggt gcagaactac gcgctggtgt atgagcccgc
     7381 caagctcgcc gacggcgtgt tcatcggccc tgccgtcgtg ctgaccaacg acacctaccc
     7441 gcgtgcgatc aacgccgacg ggacgatcaa gagcgctcat gactgggagc cggtcggcgt
     7501 cacgatcgag aggggcgcgg cgatcggcgc acgtgcaacg tgtgtggccc ccgtgacgat
     7561 cggcgcctgg gccaccgtcg ccgccggcgc cgtcgtcgtc aaggacgtgc ccgcctatgc
     7621 gctcgtcgcg ggggtccccg cccgccgcat cggctgggtc ggggagtccg gcgtgccgct
     7681 ggtggcggga gacgacgaga cgacctggat ctgcccgacc acgggcgatc gctacttcga
     7741 aaccaatgga accctgacca aggaggccgc gtgagcgagt tcattccccc ggcgaagccg
     7801 atcatcggtg atgaggagcg cgaggccgtc gatcgcgtgc tccgcagcgg catggtcgct
     7861 cagggtcccg aggtggccgc gttcgagcag gagttcgcag cgcacttcgt tcccggacga
     7921 cccgctgtag cggtgaactc gggcacggcg ggcctgcacc tgggcctgct cgccgccggc
     7981 gtgggagcag gcgacgaggt catcgtgccg tcgttcacct tcgccgcgac gggcaactcg
     8041 gtcgcgctca cgggtgggac gccggtgttc gtcgacatcg agccggagac attcacgctg
     8101 gatcctgagg cggtcgctgc ggcgatcacc cccaggacca agggcatcct gcccgtccat
     8161 ctgtacggtc acccggctca catgcgcgag ctggagaaac tcgccgccga ccgcggtgtc
     8221 gcgctgtatg aggacgccgc gcaggcgcac ggcgcctcgc tggatggacg cccggtgggc
     8281 tccttcggcg agttcgcgat gttcagcctg tacccgacca agaacatgac cagcggcgag
     8341 ggtggcatgg tcaccaccgc gaccgccgag atcgcgcgtc gggtgaagtt gctgcgcaac
     8401 cagggcatgg agcgccagta cgagaacgag gtcatcggat tcaacgcccg catgaccgac
     8461 atccacgcag cgatcggccg cgtgcagctg accaaggtgg acgcctggac gaagacccgt
     8521 cagcagaacg cggctttcct ggacgcgaac ctgcgcggcg tcgtagcgcc gccggtcgcc
     8581 gagggcgccg tgcacgtgta ccaccagtac accatccggg tccccgagga ccgcgacggg
     8641 ttcgtgaagg ccctcaagga ggagcaccag gtcggcgcgg gcgtgtacta cccgatcccg
     8701 aaccaccggc tgccctcgct cgcgccgtac gcccccggcc tggacctgcc ggagaccgag
     8761 cgcgccgccc gcgaggtcgt ctccctcccc gtgcacccct ctctgtcgca gggtgacctc
     8821 gaccgcatcg tcgccgcggt gaacgccgtc gccggagcgg gcgcctgatg gcggtgctgc
     8881 gggcgggcct gctcggagtc gggatgatgg gtcgtcacca cgcgcgtgtc ctgcgcgagc
     8941 tggatggcgt cgacctggtc gggatcgccg acccgggcgg agacccgcac ggggtcgccg
     9001 ggaacctgga ggtgctcccg gacatcgatg cgctcatcgc ggtcggcatc gacatcgcgg
     9061 tcgtcgcggt tccgacgcgg ttccacgagg acgccgcgct gaagctcgcg gccgcgggcg
     9121 tgcacacgct cgtggagaag ccgatcgcgc acagcctgga ggccggccag cgcatggtcg
     9181 acgccttcaa ggccggcggc ctggtgggcg ccgtggggca catcgagcgc ttcaaccccg
     9241 cgcttcagga gatgcgccgt cgcatcgagt caggtgagct gggcgccgtg taccaggtcg
     9301 ccacgcgtcg ccaggggccg ttcccgtcgc gcatcgccga cgtcggcgtc gcgaaggatc
     9361 tcgcctcgca cgacgtcgac ctcaccgcgt gggtggtgca gtccgactac gaccacgtct
     9421 tcgcgcagac cgcgttcaag tccggtcgtg agcacgagga catgatcgtg atcacgggcc
     9481 gcaccaagtc gggcgtgatc gtgagcaaca ctgtcaactg gctctccccg atgaaggagc
     9541 gcgtcacggt cgtcacggga gagaagggcg cattcgtcgc cgacacctcc accggcgacc
     9601 tgaccttcta cgccaacggc accatcccgc tggagtggga gtcggtgtcg acgttccgcg
     9661 gcgtgtccga gggcgacgtc actcgctacg cgttcgccaa gcgcgagccc ctcaaggtag
     9721 agcacgaggc cttccgcgat gcggtgctgg gccagagcac ggacgtcgtg accatggagc
     9781 agggccagca gacccttgcg gtcgtcgagg ccgcgctgga gtcggcccgc accgccgccg
     9841 cgcatcgtct ctgatccgtc ccgcatcact catcagaagg cagaccacac gatggtgaag
     9901 atcctcagcg tcgtcggcgc gcgcccgcag ttcgtcaagc tcgcgccagt cgcgcacgcg
     9961 atggtcggca gggccgagca catcgtggtg cacaccgggc agcactacga cgagctcatg
    10021 agcgacgtgt tcttccgcga cctcggtatc ccggctccgg acgtgaacct cgccatcggc
    10081 tcggggaacc acggcgcgca gaccggtgcg atgctggagg gcatcgagga ggtgctcctc
    10141 gagcgccggc ccgactgggt gctcgtgtac ggcgacacca actcgaccct cgcggccgcc
    10201 gtggcggcca tcaagctgca catcccggtc gcgcatctcg aggccgggct gcggtcgttc
    10261 aaccgccgga tgccggaaga gcacaaccgc gtgctcaccg atcacgcctc cgatctgctg
    10321 ctggcgccca ccgcggtcgc ggcggcccac ctgagcaacg agggcctggc cgagaagacc
    10381 gtcgtcgttg gcgacgtgat gaccgacgtg ctcaccggcg tccgcgatcg tgtcgcaggc
    10441 gacccgctcc ccgacctcgt ctcggagggc gactactacg tggccacgat ccaccgtccc
    10501 gacaacacgg acgacgccga ccacctccgc ggcatcgtcg acggtctggc tgcactggac
    10561 aagcccgtac tgctgctcgc gcacccccgc ctcgtgcaca ccgcggagag gctgggtgtg
    10621 gcgctggagg gcggttccct ccagacgcgc gcacccctgg cctacccgca gctggtccgg
    10681 gctgtcatgg gcgcggctgg cgtgatcacc gactccggcg gcctgcagaa ggaggcgttc
    10741 ctgctccgca cgccagcgac cacggtgcgt cctgaaaccg aatgggtgga gaccgtcgac
    10801 ctcggctgga acgtgctcgt cgcgccggag cgcgacgcga tcgtggaggc cgtacatcgt
    10861 cctgcgccgc acgccacgga ggagaccccg tacggcgacg gcagcgccgc cgtacgggtc
    10921 gtggatgagc tcctcgcccg atcctgacgg tcgctgtcgg ctctgaaggc cgtcggtggc
    10981 tcagtcgaga agtcgggaga ccaggttcag gaggatctca gtctgccgct ctgaggagag
    11041 gtcgcgcgcg gacctctccg aggccgcttt gtactcggcg acggcctccg gcgtcagcgc
    11101 gtcgatcgcc gcgtgcaggg agtcggccgt gaagtcgtcg gcgacgacac ccacgcccgc
    11161 ctcccgcacg atccgcgcca tctcggggga gggcccgatc accacgccga gccgggcctg
    11221 cacgtagtcg aagagcttgt tcggcaacgc gtgcaggtgg ttgaaattga tcggcgggag
    11281 cacgtgcagc cccagatcat ggtcggccag cgtcgcgatg agctcgtcgt acggcaccgg
    11341 ctcgtgcacg cggatgcggg cgtcggcgga ggcgcgctcc cggaggccgc ggccgtaatc
    11401 ggagtccgct ccgaccaggt acaggtcgag cgtgacgtcg gcttcggacg ccttcacggc
    11461 gtcgatgagg atctcgacgt gccggtgcgg cgccgcgacc ccactgtgca ccaggcgcag
    11521 cggggcgtgc acgggcgcgg cctggcgctc ctggtacggc gaggcgttga tgatcagcgt
    11581 cgggtcgagg tcgtgctctc ggcggtactc ctcgacgatg cctgagctca cggtcgtggt
    11641 tgccgcggca cggtgcaccg gaccgctcag gatccaccgg aagtacggcg cgatcagcag
    11701 acgccaggcg agggagtgct cgttctgccg ggggaagtac tcgtgcaggt cgacgagcac
    11761 tccgcggcgg gacctcagag tctccgcgac cgtcgcggtc tgcacgtcgt gggcgatcac
    11821 gaggtcccag tcgcggtcgc cgagcgcggc gcggatcgcg tgatcctgcg cgctgagacg
    11881 cgtgatcagc ggataaaggt gcagcgcgag caccatcagg tacagtatcc ggcccagggc
    11941 gccccaccgc tgctcgcggt ccggcaggcg cacgtgctcg atctccggga acggcgagtc
    12001 cccgcgggac gcggtcacga tgtcgtgatc accgcgcagg gcgcgcacct gcttgagggc
    12061 ccgcggagat cgcgccagcg gggccgggct gaggatcgcg atccgggggc gtgcgtgcgc
    12121 agcagccgcg gccgtcatgc cgaggcgtcc gagcgggaga agatgcggtc gaccatgcgg
    12181 cgccacacct tcacctggac ctcgctcgag agctcgcgcg cctgacggtg agcgctggcc
    12241 ttccaggcat ccaccttggc ggcgtcgatc ctgtccagca gagcggccag cgattcgcca
    12301 gtgaactcct cggtcaccgc gccgaggtcg tgttcgcgcg tgatcctggc catctccggc
    12361 gacggtccgg tgatgatggc cagtcgggcc tgaatatagt cgaagaactt gttcgggagg
    12421 caccacagca ggttgaacgt cgacggggcg atcatcgcca ggccgatgtc gtactggctc
    12481 agggtcgtga cgagctggtc gtacgggacc ggatcgcgga agcggatgcg ggggtcgccc
    12541 tccgccatct tccgcaggga cgcgaagtac gcgtcctccg cgccgaccag atacaggtcc
    12601 agtgtcactg gggtacgggt gagcttgacg gcctcgatca tgatctcgag cttgcgcgtc
    12661 gcggcgacgg cgccactgtg caccatgcgg atcggctcag gtgtcttcga gggctcgatg
    12721 tcgcgatacg gggtggcgtt gaccaccaga tcgcattcga tcccgaactc gcgccggtac
    12781 tcgtcgacga tgccctggcc gaccgtgctg accgcggcgg ccctgcgcac atggcgcttg
    12841 aggatccatc ggaagtagcc gccctcgacg aggcgccatc gcagcgagtt ctcgccctgc
    12901 cgggtcgcgt actcgtgcag gtcgacgagc acgccgaagc gggagcgcag cttgcgcgcg
    12961 accgggacgg tggacacatc gtgggcgatg atcaggtccc actcgtgcgg ggtcagccgt
    13021 gccagggcgg aggcgttccg cgggagcacc ttggacagca gccggtactg gcgaagcagg
    13081 atgatcaggg agtacatgcc ccggatcgcg agcagtccgt gggaggtggg gagcgactcg
    13141 agttcgatgt gcgggacgcc ctccaccggg gccgggccga atccggcggt ggtcacatcg
    13201 tggtcgtcgc acagcagctc gatctgcttc agcgcacggg gctcccgatt gatcggggtg
    13261 aaggtgaaga ccaggatctt cttccgtgcg gatccctgca tgtcggctcc tgttcgtcgg
    13321 ttgtcagctc gggcggagcg atcggatgtc ggcggcgggg ggataggtga tcagcacggc
    13381 gcggtgcttt cccgtcatca cgaacatggt ccccgcggcg acagcggatg ctccggcggc
    13441 cagtgccggc gcgaagtcag ccagggatcc tgcgccgccc agggcgacga tcgggatggt
    13501 cacggcatct gccacgctct ggatcagctt cagatcgtac cctgagcggc tgctctcacg
    13561 gtcgatggac gagatcatca cctcgccggc gccgtactgc tgcgcctgcg ccgcggccgc
    13621 agccggggtg agccccgtct ttcgggaggc gcgagccgtg taagtgtccc agccgccggt
    13681 gagccgccgc ttggcgtcga cgctcaccac cgtgctcgat gagcccagcg cctcggacac
    13741 ctcggtgatc acctcgggac gcgcgaaggc ggcggtgttg agcaccacct tctcgatgcc
    13801 gatccccgtg atgcgccggg cctcatccgc tgacgcgaca ccgcccccgt agccgactgg
    13861 catgaacgcc tcgctcgcga tctcctcgat cagcgtgtaa ttgactccgg tgcccttggc
    13921 cgaggcatcg atgtcgcaga tcagcagctc gtcgacctgt ttctcgttga agatacgcac
    13981 cgcgttgatc gggtcgccga tgtatgtgtc gccgctgaac ttcacgggct tgacgaggta
    14041 tccatcgctg acgagcagca cggggatcac gcgcgggaag agagtcatag tgcggcgaag
    14101 ttcctcatga tcgtcatgcc gaaggtgtgg ctcttctcgg ggtggaactg cgcgcccata
    14161 acgttccccg tcctgaccat cgaggagaac gggatgccgt agtcggtgat cgcgagagtg
    14221 tcctcggccc gctcgggaac cacgtggtag ctgtgcacga agtagaaccg gctctcctcg
    14281 ggcagctccg ccaccagcgg gtcctcgcgg gccgggttcg ccaggttcca tcccatgtgc
    14341 ggcacgcgga tgccgagctt ctcgtcgaag cgcacactgc gtccggggat gagtccgagc
    14401 ccgctcttca cgccctcgtc gctcccgtcc aggagcagct gcatgcccaa gcagatgccc
    14461 agcagcggac gtccggtgcc cgcgaagtcg cggagcgcgt cgatcagccc cgcttcggcc
    14521 agacgcgtca cgcccgtgtc gaaggcgccc accccgggca ggagtacctt gtcgacctcg
    14581 gcgatctcgt ccgcggtgcg gaccgagacg gactgcgcgc cgacgcgctt gagcatgttc
    14641 cggacggagc cgaggttgcc gagtccgtaa tcgacgatgc cgaccgtcac gcggccttct
    14701 tcataccgag gctcttcatg atcctggctg gcacccgcag cacgctgagc agcaggttcc
    14761 acgacttctc cgagttcggg tacgccgtgt agtccaccgg gggagcatcg aagatgcgtt
    14821 ccagctccgc tgtcgagacc ccgagcttct tggcgatgaa ctcgtgatcc tgcgcccgca
    14881 gatcctccgg gtagttctcg gtctccatca gagtgagagc ctcgtcccgg ccgagctggt
    14941 gggagacgat cagcgaggag tagtgcgcga ggcgcttgtc gaacccgaac ttgtgaggca
    15001 ggtagtagcc ctggaagtag cgggtgaaca ccgactcgta gtgcttgccg ccgtagtcgc
    15061 gccagccgac ccgctccgtg atggcggcct tggcgtcctt gtagacgtag ggcatcaggt
    15121 tcagcggacg ctcggtcctg atcccgcgga catacgggta ccagatgtgc cgcttgaagc
    15181 ggccgatgat cgggtaggtc ttcagcttca ccgagccgaa gcgcttctgg atcgccttga
    15241 ggtgcttggc gtcgtcggac gagtagcccc aggcggaggg caggatcgat tcgctggcgt
    15301 agttcgatcc cgagaggatg tacttgatgc cgtacttctt ggcctggtcg aacgcgaccc
    15361 agccgaaggc gtgatcggtg gggatgtcgc agttcgcgac cgacgccttg aagaacgaga
    15421 gctggaggtc cttcatctcg cgccagtcga cgacgtgggt gtagaggtcg accccggtgg
    15481 cgttgatcag gttctggatg ttcccgacgg cgagctcgga gttccagccg ctgtcgaagt
    15541 gcacggcgag cggacgcagc ccgagctcga ccgcgcgcat aagcaggtag gtgctgtcga
    15601 caccgcccga gacgccgatg atgcagtcgt acggcttgcc gcgaccggac tccttgatcc
    15661 tcgcgacgag cgcctgcagc tcgggctcgg cctccccgga gagcgcccgg tcgacgacct
    15721 tgcccatgta ctggtcgtag tcgagcgcgt agttgctcac gccctcagcg tcgaacgtga
    15781 tgttcggatc ggttgtgtcc atgatggtgc gagtgcaccg ctgatagggg cgtgttccac
    15841 ccgtcatccg gggatcctcc aagggtcgac tcgtcgccgc gtcagtcgga cgatgccgac
    15901 aaaatcacaa ctcttcacga taccatcggg ggttgtgtca gagagtcagc aagcacacag
    15961 cgaatcccgg ccgcagatcc tcagcatctc cttctcgccc ctcgatcggg acgcccgtgt
    16021 gctccgtcag atcgcggtgc tgagcgagtt cggcgatgtg accacggtgg ggttcgggcc
    16081 cgcgcccgcg ggcgcggcac gacacatcga ggtgcccgcg ggactgccgt ccctgccgca
    16141 gacgccggcc ggcgtgctga agct
//
LOCUS       DATMSX010000504         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_26532,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000504 DATMSX010000000
VERSION     DATMSX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26532"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN041_16230"
     CDS             <1..265
                     /locus_tag="VN041_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HWU30629.1"
                     /translation="GGVVVMLGLQRAGEVPAPIATAITRELTLRGSFRFAAEFDDVIE
                     ALATGRIDVTGIVSDAMPAAHALPAFALAADASRSCKVLLDFR"
     gene            265..762
                     /locus_tag="VN041_16235"
     CDS             265..762
                     /locus_tag="VN041_16235"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HWU30630.1"
                     /translation="MRVVVMGPSGCGKSTVGRALADELGARFVDGDDLHPSANVEKMA
                     AGVPLTDDDRMPWLATVGAELGSDARIVIACSALRRVYRDAIRESEPGVFFAELTASR
                     DELVRRMGERGAHFMPTSLLDSQLATLESLQPDESGVRIGGLAPADAVAASIAGELTR
                     TQSLR"
     gene            complement(747..872)
                     /locus_tag="VN041_16240"
     CDS             complement(747..872)
                     /locus_tag="VN041_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30631.1"
                     /translation="MPVAAVITHVLFWLIGAMAVVGGIGAFAAMFTMGRSGYRKD"
     gene            1062..1673
                     /locus_tag="VN041_16245"
     CDS             1062..1673
                     /locus_tag="VN041_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWU30632.1"
                     /translation="MSDDNKVHADLRTSFGKGFARRLRAEGKIPAVIYGHGTEPVHVA
                     LPGHQVSLIIRRANALLDLDIEGKSQLALVKDVQKDPVRQIIEHIDLLVVKKGEKVSI
                     DLPVVLVGESFAGTIANLDATTLAVEAEATHIPEHVEVSVEGLEEGAHITAADVKLPK
                     GVSLVADPETLVVAISVPAAPAEEEAPAEEAAAEAPAEEAAAE"
     gene            1743..2324
                     /gene="pth"
                     /locus_tag="VN041_16250"
     CDS             1743..2324
                     /gene="pth"
                     /locus_tag="VN041_16250"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWU30633.1"
                     /translation="MASTWLIVGLGNPGPRYAATRHNIGQMVVDELAERRGERFREHK
                     AGARVVETWLRPGGDKLVLAKLNSFMNVSGAPVAALARFYSVEPDHVVVIHDELDIPF
                     DSIRLKTGGGHGGHNGVRDIARALGTPEFPRVRAGIGRPPGRQDPADWVLGNFGSVEQ
                     KTLPIFLSDVADATELLLEEGLLAAQQKHHARG"
     gene            complement(2347..2889)
                     /locus_tag="VN041_16255"
     CDS             complement(2347..2889)
                     /locus_tag="VN041_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30634.1"
                     /translation="MTDGSGPVPSGQVPPPPVPVPPPAAPPGAYGVPVGGYQMPVGGY
                     EVPSDTPPASRRTGLLALVAALIAVVVAPIVAGILAVQIGMQVPLESLITVTGDVVWA
                     ALTPVRTLVLWIEILFWAATAIGILALALGIVATVRRRGRGAGITAMILAVLGPVVFF
                     LLTSVLLGVGNGMAAVPPAG"
     gene            complement(2886..3896)
                     /gene="trpS"
                     /locus_tag="VN041_16260"
     CDS             complement(2886..3896)
                     /gene="trpS"
                     /locus_tag="VN041_16260"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWU30635.1"
                     /translation="MTQKPRLYSGMQPSADSLQAGNYIGALLQWREMQSSFDAFFSVV
                     DLHAITVPQDPAELREKTRRTAAQYIAAGIEPSKSTLYVQSHVRAHAELAWILSTITG
                     FGEAGRMTQFKDKSARYGTDSTSVGLFTYPVLMAADILLYQTELVPVGDDQKQHVELT
                     RDLAERFNKRFGETFTIPKAVIQKDTARIYDLQNPTSKMSKSAESDAGVLWLLDDPAK
                     SAKKIMRAVTDAEGSVHYDRENKPGVSNLLTIYAALTGRQVGSIEDEYAGRGYGDFKK
                     GLAEVVVDEFGPVRERANALLDDPAELDRLLAVNAAKADEVANATLADVYDKVGLLRR
                     VL"
     gene            complement(3913..>4165)
                     /locus_tag="VN041_16265"
     CDS             complement(3913..>4165)
                     /locus_tag="VN041_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWU30636.1"
                     /translation="SEAGGTGLGWVDVGRAAHGEVAGPYTWWSMRGQAFDNDSGWRID
                     YHLATPPLAARATGYTVARATTYAERWSDHAPVIVDYTY"
BASE COUNT          637 a         1438 c         1469 g          621 t
ORIGIN      
        1 gggcggcgtc gtcgtgatgc tgggcctgca gcgcgcgggc gaggtgccgg cgccgatcgc
       61 caccgcgatc acccgcgagc tcacgctgcg aggctcgttc cggttcgccg cggagttcga
      121 cgacgtcatc gaggcgctgg ccacaggccg catcgacgtg acgggcatcg tcagcgacgc
      181 gatgcctgcc gcgcatgcac tccccgcgtt cgcactcgcc gcggacgcat cgcgctcctg
      241 caaggtgctg ctggacttcc ggtgatgagg gtcgtcgtga tggggccgag cggatgcggc
      301 aagtccacgg tgggcagggc cctcgccgat gagctcggcg cccgcttcgt ggacggcgat
      361 gatctgcatc cctccgcgaa cgtggagaag atggcggccg gtgtgccgct gacggacgac
      421 gaccggatgc catggctggc gacggtcgga gccgagctcg gctccgacgc ccggatcgtg
      481 atcgcctgct cggcgctgcg ccgggtctac cgcgacgcca tccgggagag cgaaccaggg
      541 gtgttcttcg ccgagctgac cgcgtcccgc gacgaactcg tgcgacgcat gggggagcga
      601 ggcgcccact tcatgccgac atcgctgctg gactcccagc tggcgaccct cgagtcgctt
      661 cagccggacg agtcaggagt gcggatcggc ggactggccc cggccgacgc cgtggcggcg
      721 tcgatcgcgg gcgaactgac gaggactcag tccttgcggt agcccgagcg acccatggtg
      781 aacatcgcgg cgaacgcgcc gatgccgccg acgacggcca tcgcgccgat gagccagaac
      841 aggacgtggg tgatgactgc agcaacgggc atggcacaca gcctatcgag gattcgggta
      901 ccatggagaa gttcctcggc gagggatgcc gtctccgcgc gcgcatccgt tatcgacgcg
      961 gtgatgtgag cactgatgtc tcccttcttc gtgtccgcgc tcgaggcgcc gaaacccacg
     1021 accaccgcac ggccagcccc gtgtgcttga aggagaacat catgtccgac gacaacaagg
     1081 tccacgcaga cctccgcacc agcttcggca agggcttcgc tcgtcgcctg cgcgccgagg
     1141 gcaagatccc cgcggtcatc tacggccacg gcacggagcc cgtgcacgtc gcgctgcccg
     1201 gccaccaggt ctcgctgatc atccgtcgcg ccaacgcgct gctcgacctc gacatcgagg
     1261 gcaagtcgca gctcgcgctg gtcaaggacg tgcagaagga cccggtgcgc cagatcatcg
     1321 agcacatcga cctgctggtc gtcaagaagg gcgagaaggt ctcgatcgac ctccccgtcg
     1381 tcctggtggg cgagtcgttc gcgggcacca tcgccaacct cgacgccacg accctcgcgg
     1441 tcgaggccga ggccacgcac atccccgagc acgtcgaggt ctcggtcgag ggcctcgagg
     1501 agggcgctca catcaccgcc gccgacgtga agctccccaa gggcgtctcg ctggtcgccg
     1561 acccggagac cctggtcgtc gcgatctcgg ttccggcggc gcccgccgag gaggaggctc
     1621 cggccgagga ggctgctgct gaggctccgg ccgaggaggc cgccgcggag tgatccgctg
     1681 atgcattcgg agggggcgcg agcgatcgcg cccccttctt catcccacga gaggaatctg
     1741 gcatggcatc cacgtggctc atcgtcggtc tcgggaaccc gggtccgcgg tatgcggcga
     1801 cccgccacaa catcggtcag atggtcgtgg acgagctcgc cgagcgccgg ggcgagcggt
     1861 tccgcgagca caaggcgggc gcccgcgtcg tggagacctg gctgcgtccc ggtggcgaca
     1921 agctcgtgct cgccaagctc aactcgttca tgaacgtctc gggcgcgccg gtcgcggcgc
     1981 tcgcccgctt ctactcggtc gagccggatc acgtcgtcgt gatccacgat gaactcgaca
     2041 tccccttcga cagcatccgc ctgaagaccg gcggcgggca cggcggccac aacggcgtcc
     2101 gggacatcgc ccgtgctctc ggcactcccg agttcccgcg cgtccgggcc ggtatcgggc
     2161 gtccgcccgg tcgccaggac cccgcggact gggtgctcgg gaacttcgga tcggtcgagc
     2221 agaagacgct cccgatcttc ctctcggatg tcgcggacgc caccgaactg ctcctcgagg
     2281 aggggctgct cgccgcgcag cagaagcacc acgctcgcgg ctgagcgcgc ctcggggagg
     2341 tggaggtcag cccgccggcg gaaccgctgc catcccgttt ccgacgccca gcagcacgga
     2401 cgtcagcagg aagaagacga cgggacccag cacggccagg atcatggccg tgatcccggc
     2461 gccgcgcccg cgacggcgca ccgtcgcgac gatcccgagg gcgagcgcga ggatgccgat
     2521 ggccgtcgcc gcccagaaca ggatctcgat ccacagcacc agggtgcgca ccggcgtgag
     2581 cgcggcccac acgacgtccc ccgtgacggt gatcagggac tccagcggca cctgcatgcc
     2641 gatctgcacg gcgaggatgc ccgcgacgat cggcgcgacg accacggcga tcagcgccgc
     2701 gacgagcgcg agcaggcccg tgcgccggct cgccggagga gtgtcggacg gcacctcgta
     2761 gccgccgacg ggcatctgat agccgcccac cggcacgccg tacgcaccgg gaggagcggc
     2821 gggcggcgga acgggcacgg gcggcggggg aacctgaccg gacgggaccg gccctgatcc
     2881 gtcggtcaca gcaccctccg cagcagcccg accttgtcgt agacgtcggc gagggtggcg
     2941 ttcgcgacct cgtcggcctt cgcggcgttc acggccagca gccggtcgag ctcagcgggg
     3001 tcgtcgagca gcgcgttcgc gcgctcgcgg acggggccga actcgtcgac cacgacctcg
     3061 gcgaggccct tcttgaagtc tccgtagccg cggcccgcgt actcgtcctc gatcgaaccg
     3121 acctggcgtc cggtgagggc cgcgtagatg gtgagcaggt tggagacgcc gggcttgttc
     3181 tcgcggtcgt agtgcaccga gccctcggcg tcggtcaccg cgcgcatgat cttcttcgcc
     3241 gacttcgccg ggtcgtcgag cagccacagc accccggcgt cgctctccgc cgacttcgac
     3301 atcttcgacg tcgggttctg caggtcgtag atgcgggcgg tgtccttctg gatcacggcc
     3361 ttcgggatcg tgaacgtctc gccgaagcgc ttgttgaacc gctccgcgag gtcgcgggtc
     3421 agctcgacgt gctgcttctg gtcgtcgccg accgggacca gctcggtctg gtacagcagg
     3481 atgtcggcgg ccatcagcac cgggtaggtg aacagtccca ccgaggtcga gtcggtgccg
     3541 tagcgcgccg acttgtcctt gaactgcgtc atgcgcccgg cctcgccgaa gcccgtgatg
     3601 gtgctgagga tccaggccag ctcggcgtgc gcgcggacgt gcgactgcac gtacagagtg
     3661 gacttggacg gctcgatgcc cgcggcgatg tactgcgccg cggtgcgacg ggtcttctcg
     3721 cggagctcgg cagggtcctg cgggaccgtg atcgcgtgca ggtcgacgac cgagaagaag
     3781 gcgtcgaacg aggactgcat ctcacgccac tgcaggagcg ccccgatgta gttgccggcc
     3841 tggagggagt cggcggaggg ctgcattccg gagtagaggc gtggtttctg agtcaccgtt
     3901 caatcctatg gttcagtacg tgtagtccac gatcaccggt gcgtggtcgc tccaccgctc
     3961 ggcgtaggtg gtggcgcgcg cgacggtgta cccggtggcg cgggccgcca gcgggggagt
     4021 ggcgaggtgg tagtcgatgc gccatccact gtcgttgtcg aacgcctggc cccgcatcga
     4081 ccaccacgtg tacgggccgg cgacctcgcc gtgagcggcg cgcccgacgt cgacccaccc
     4141 gagccccgtg ccgccggcct cggac
//
LOCUS       DATMSX010000505         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_187764,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000505 DATMSX010000000
VERSION     DATMSX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187764"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VN041_16270"
     CDS             complement(<1..636)
                     /locus_tag="VN041_16270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU30637.1"
                     /translation="MTTDLTRLRTLLDEGTPVNWVCTGDSITHGLIHTQGARNYVDHL
                     HELIRGDMSRVQDVIIDTAISGWRIVQLLEDFDRRVAAWHPHVVTLMIGTNDCSTAGV
                     FPVIEPAEFAASVTEFVARVRALGAIPVLQTQPAIDVRNAPERARIAEFAQAIRDVAA
                     AQDVILIDQFARFAEMGRGHVNDVAWGLLGDPFHPNAAGHAVLALGVATALE"
     gene            complement(709..1947)
                     /gene="manD"
                     /locus_tag="VN041_16275"
     CDS             complement(709..1947)
                     /gene="manD"
                     /locus_tag="VN041_16275"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HWU30638.1"
                     /translation="MTIDKAEVIVTSPNRNFVTLKITTSDGVTGLGDATLNGRELAVV
                     SYLRDHVVPLLIGSDENRIEDTWQFLYRSAYWRRGPVTMAAIASVDMALWDIKAKKAG
                     MPLYQLLGGVSRTGLLAYGHASGTDLPSLFDSVRDHLEQGYRAIRIQTGVPGLNAIYG
                     IASQSAAAGGGDERYDHEPARRGAKPVEEDWDTRAYLRHLPGVFEAVRNEFGPELPLL
                     HDGHHRMTPIQAAKLGKELEPYDLFWLEDCTPAENQEALRLVRRHTTTPLAIGEIFNT
                     VWDFKDIIREQLIDYVRGAVTHMGGISPLKKTLEYAAMYQIKSGMHGPTDISPVGMAA
                     AMHLGLSIHNFGIQEYMKHGERTNQVFQQSFTWQNGLLHPGDAPGLGVELDLDEAGKY
                     PYEQAYLPYNRLADGTVHDW"
     gene            complement(2045..>2522)
                     /locus_tag="VN041_16280"
     CDS             complement(2045..>2522)
                     /locus_tag="VN041_16280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWU30639.1"
                     /translation="LTVLAHDPFADADQAASLGVTLVTLEELFERSDVVSLHAPLLPE
                     TTGTIGRALLERMPQGAVLINTARGGLIDEQALVDVLRRRPDLTAQLDVTVVEPPAAD
                     SELWGLTNIELTPHVAGSMGDDRGAMGRLVADELGRLAQGLPLQHRVGRDQLARMA"
BASE COUNT          360 a          836 c          919 g          407 t
ORIGIN      
        1 ctcgagcgcc gtcgcgacgc cgagggcgag cacggcgtgc ccggccgcgt tcgggtggaa
       61 cgggtcgccc agcagtcccc aggcgacgtc gttcacgtgg ccacggccca tctcggcgaa
      121 gcgcgcgaac tggtcgatga ggatcacgtc ctgcgcggcc gcgacatccc tgatcgcctg
      181 ggcgaactcg gcgatgcggg cgcgctccgg ggcgttgcgc acgtcgatcg cgggctgggt
      241 ctgcagcacg gggatggcgc cgagtgcgcg cacccgtgcg acgaactcgg tgacggatgc
      301 cgcgaactcg gccggctcga tcacggggaa cacgcccgcg gtggagcagt cgttcgtgcc
      361 gatcatgagc gtgacgacgt gcggatgcca ggcggccacg cgccggtcga agtcctcgag
      421 cagctgcacg atgcgccagc cgctgatggc ggtgtcgatg atgacgtcct gaacgcggga
      481 catgtcgccg cggatcagct cgtgcaggtg gtcgacgtag ttgcgcgcac cctgggtgtg
      541 gatgagtcca tgcgtgatcg aatcgccggt gcacacccag ttcacgggcg tgccctcgtc
      601 gagcagggtg cgcaggcggg tcaggtcagt ggtcacgcgg atgctcctgt cgggatggtg
      661 cttccatcct ggcgcatccg gcttccgggc ctcgccgggc acgccccgtc accagtcgtg
      721 gaccgtcccg tcggcgagcc ggttgtaggg caggtacgcc tgctcgtacg gatacttgcc
      781 cgcctcgtcc aggtcgagct ccacacccag acccggggcg tcgccggggt gcagcaggcc
      841 gttctgccag gtgaacgact gctggaacac ctggttcgtg cgctcgccgt gcttcatgta
      901 ctcctggatg ccgaagttgt ggatcgacag gccgaggtgc atggcggcgg ccatgccgac
      961 cggggagatg tcggtcggcc cgtgcatgcc cgacttgatc tggtacatcg ccgcatactc
     1021 gagggtcttc ttcagcgggc tgatgccgcc catgtgggtg acggcgccgc gcacgtagtc
     1081 gatcagctgc tcgcggatga tgtccttgaa gtcccacacc gtgttgaaga tctcgccgat
     1141 cgccagcggg gtggtggtgt gccggcgcac gaggcgcagc gcctcctggt tctcggccgg
     1201 ggtgcagtcc tccagccaga acaggtcgta gggctcgagc tccttgccga gcttggcggc
     1261 ctggatcggc gtcatgcggt ggtggccgtc gtgcagcagg gggagctcgg ggccgaactc
     1321 gttgcgcacc gcctcgaaga cgccggggag gtggcgcaga taggcacggg tgtcccagtc
     1381 ctcctccacc ggcttcgcac cgcgacgtgc gggctcgtgg tcgtagcgct catcgccacc
     1441 gcctgccgcg gcggactgcg aggcgatgcc gtagatcgcg ttcaggccgg ggacgccggt
     1501 ctggatgcgg atggcgcgat agccctgctc gagatggtcg cgcaccgagt cgaagagcga
     1561 gggcaggtcg gtgccggacg cgtggccgta ggcgagcagg ccggtgcggg acaccccgcc
     1621 gagcagctgg tacagcggca tcccggcctt cttcgccttg atgtcccaca gcgccatgtc
     1681 gacggaggcg atcgccgcca tcgtgacggg gccgcggcgc cagtacgccg agcggtacag
     1741 gaactgccag gtgtcctcga tgcggttctc gtcggaaccg atcagcagcg ggacgacgtg
     1801 gtcgcgcagg tacgagacca cggccagctc gcggccgttg agggtcgcgt cgcccaggcc
     1861 ggtcacgccg tcggaagtgg tgatcttcag cgtcacgaag ttgcggttcg ggctggtgac
     1921 gatcacctcg gccttgtcga tggtcatgga ttctccggat gctgagggcg cgctgctgcg
     1981 cgcggcgatg ctggcatcga catgaaacca cggatcacca cggtaaggca atcggttgcc
     2041 aacctcaggc catgcgagcc agctgatcgc gccccacccg gtgctgcagg ggcaggccct
     2101 gtgcgagccg tccgagctcg tccgcgacca gccttcccat cgcgccgcgg tcgtcgccca
     2161 tggagcctgc gacgtgcggc gtgagctcga tgttcgtcag cccccacagc tccgaatcgg
     2221 ctgccggcgg ctccacgacg gtgacgtcca gctgggccgt cagatcgggg cgccggcgca
     2281 gcacgtccac cagggcctgc tcgtcgatca ggccgccgcg ggcggtgttg atgagcacgg
     2341 cgccctgcgg catccgctcc agcagcgcgc gcccgatcgt ccccgtggtc tccggcagca
     2401 gcggggcgtg caggctcacc acgtccgacc gctcgaacag ctcctccagg gtgaccaggg
     2461 tcacgccgag cgacgccgcc tggtcggcat ccgcgaacgg gtcgtgcgcg aggacggtga
     2521 gc
//
LOCUS       DATMSX010000506        13419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_93339,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000506 DATMSX010000000
VERSION     DATMSX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93339"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN041_16285"
     CDS             <1..726
                     /locus_tag="VN041_16285"
                     /inference="COORDINATES: protein motif:HMM:NF018390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 family protein"
                     /protein_id="HWU30640.1"
                     /translation="RTSPTSRPTTASTAQPDRIGYVSDADGSTVAMAAALRLLRLAAA
                     GAQLPGDVIVTTHIATHVSITPREPADFMGMPVSSETMNLHEVDAAMDAILSIDASKG
                     NRLINHPGVAISPTAMAGYLLPVAPGLLTILEQATGAPARTFPLALQDITPYSNGYRH
                     FNSIMQPTVATSAPVVGVALTAAAVVPGSSTGASYEPALQEATRFVVETAKQFTQDRV
                     SFFDIDEYDRLVAQYGDLTRFQS"
     gene            793..2436
                     /locus_tag="VN041_16290"
     CDS             793..2436
                     /locus_tag="VN041_16290"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWU30641.1"
                     /translation="MTSSPSTEQLPPKTHPRAFEPITLIVTVVLSVLGAVIGVHLITT
                     LGISANTSVIGAVIAMLLGRITFFGFAKFRSKHRQNLIQSAVSAATFAAANGLLAPIA
                     IPWAMGKPELVWPMLLGASLGVLVDVFVLFKAFGSKFLPASAAWPPGVAAAETILAGK
                     EGGRRALILVGGGVIGVIGVIGSLFALPMSAAGVALIGNIWALLMFAVGLLISQYAPV
                     LFQINLGALYIPHGVMIGAGVVALLQVMWIIFGARTKQQQAQEARHTEAIKLDETLDY
                     TISRKGLGRALGLGYVAFVVGALVIALVGGVASDLSIPALIGFVLFAGFAAIVHELIV
                     GLAAMHAGWFPAFAVTLIFLILGLFLQMPQVPLLVLVAYCSATGPAFADMGYDYKAGW
                     ILRREGRPWKAYELQGRREQLKASLVAIVIAIGIVAISWQGLFADGKIPPSSKVFADT
                     LAVGISDPHVALTMALWAIPGALIQLIGGSKRQMGVLLATGLLVATANAGWLVLAALL
                     FRVIWLKYRGAKGEQEIALIGAGVIAADSLMSVGKVIKF"
     gene            complement(2561..3457)
                     /locus_tag="VN041_16295"
     CDS             complement(2561..3457)
                     /locus_tag="VN041_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HWU30642.1"
                     /translation="MSGSTDPASEDPFVVHRGLLFTVAYEILGSAADAEDVLQESWLR
                     WADVDRGRVHDPRAYLVRIVTRQSLNQLRTVSRRREEYVGPWLPEPLLTSPDVADDVV
                     LAESVSLAMLTVLETLTPTERAVFVLREVFDVPFDEIADAVDKTAATVRQIAHRARDH
                     VAARRPRVEVDASEHDRVVRRFIAALDSGDMQGLMDVLAPDVVSTTDSGGLVRHAARR
                     PLFGADRVVAFLRGSIAKLDIAYRVSAMTVNGRLAARIEVGGVLMAAVSFEVEDGRIS
                     RVFSMANPRKLEALGAEHPLAR"
     gene            complement(3454..4047)
                     /locus_tag="VN041_16300"
     CDS             complement(3454..4047)
                     /locus_tag="VN041_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU30643.1"
                     /translation="MNETRIPAIPVTGLYGAVVKAAAKKMLGKVPDSLGVMWQHRPVM
                     MDMMKFGGRTEKWRELDQSLGTLASMACAATVGCSFCLDLHYFMSRDHGLAEEKAREV
                     PIWRESTRFTPLERRVMEYAEAMSQTPPAVTDELSAALLAELGAPALLELAAKVGYLN
                     ATARMNVALGVHSDGLADACGLAPLATATPAGVASHS"
     gene            complement(4241..7231)
                     /locus_tag="VN041_16305"
     CDS             complement(4241..7231)
                     /locus_tag="VN041_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HWU30644.1"
                     /translation="MHEETSLTVGRVNRVLTERVRPAIHSSRVPLEIAAHQLPGEPIA
                     PVEGLALEFEPFTAGSMWGPAWSTTWFRVRGSVPAEWSGRRIEALIDLGFDINMPGFQ
                     CEALAYRPDGTPLKSINPRNQWVAVEAEPGGAVEFYLEGAANPVLLDYHPFLPTQEGD
                     IRTSSPEPLYRVRHMDLAVFEEAVFELALDLEVLIELQAELPETSPRRMRILQALDDA
                     LDVLDLQHIVETAPDARARLADVLASPADASAHRIAAIGHAHIDSAWLWPVRETIRKV
                     ARTTSSMTTLIDEQPDFLYGMSSAQQYAWIKEHRPEVFARVKDAVAAGRFLPLGGMWV
                     ESDTVMPTGESLVRQFSHGQRFFEREFGIRSKGVWLPDSFGYSPALPQLMRRAGFEWF
                     FTQKISWNQRNVFPHHSFLWEGIDGSRMFTHFPPMDTYNSQLSGSEVAKASRQFKENR
                     KATMSIAPVGWGDGGGGTTREMTGKAARMASLEGAAQVEWKHPDDFFDDARAELTDPA
                     VWVGELYLELHRGTLTSQHATKALHRWAEQALIEAELWAATATVQRGVEYPHDELDRL
                     WQTVLLHEFHDILPGTSIAWVHREAVEVLSGVLSDAEKLSSTARQALAGEGDRELVFT
                     PTSVGGGRALGAAPVVLSTASASLTESPDGFVLSNDLVAVIVSAEGLITSAVDKATGR
                     EAIPAGKPANLFQLHQDFPNMWDAWDIDRYYKNRVDDLTAVSSISASVVDGAAVVVVE
                     RAFSESRLTQTITLRPGSRTVLLQNDIDWHETEKLLKLAFPLDVFARETLAETQFGFQ
                     RRVTHVNTSWEAAKFETSMHRFVLAQEDDGFGVALVNDSIYGYDTSREVDGDDVTTTV
                     RLSLLRAPRFPDPDTDHGRHEIEVGLVIGADAEIATAEGIRLNAPAHVVRGANEVEPL
                     VSATGSGIVVSSVKLADDRSGDVIVRVYESLGRRTTGTLTVGFEHTGIREVSLIEDEL
                     DSPRTGGELTLKPFEVRTLRIARA"
     gene            7322..8347
                     /locus_tag="VN041_16310"
     CDS             7322..8347
                     /locus_tag="VN041_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30645.1"
                     /translation="MSRSKRVTIADIARQAGVSPGAVSFALNGRPGVSEETRQRILAI
                     ADEHQWRPSSTARALVGARAGVVGFAVNRPARTLGTEAFFTDLIAGVQSALAARRIGM
                     QLVVVPSMDEEMDTYRRWRRSNQVDGVLVLDPRVDDPRPGLLRSLGLPSITIGGQPSP
                     DDMHATLWLDDADAARTVFGYLAGLGHRSIVYISGPAEHEHVRLRSSVLAAMAERDIV
                     GEVIQTDYSPAQVSTVMRTLLSRAERPTAVVFDNDMMAIAGLRITQEMGVAVPAGLSL
                     ASFDDSIVTGLVRPSITCLTRDTFTLGETAADFLIEQLDSAEVLPDRTGPQPRLTVRE
                     STAPPRS"
     gene            complement(8372..9676)
                     /locus_tag="VN041_16315"
     CDS             complement(8372..9676)
                     /locus_tag="VN041_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWU30646.1"
                     /translation="MPAPTSADAPLRFGANYTPSKDWMHSWLSLDLDDVRRDFEGLAS
                     LGLDHVRIFPLWTVLQPNRTLIRGKAVADVRAVVDVAAEFGLDAGVDVIQGHLSSFDF
                     VPSWLFTWHDKNMFTHPDALSGQVALVETLGAALNDAGNFLGFTLGNETNQFSAETHP
                     HQWPVTEAEAANWIASLLDAAERAAPGRDHLHSEYDAAWYMDGHGFTPALASRLGAMT
                     TVHSWIFNGTAQRYGGRSAASDRHAEYMIELSRAFATDPARPIWLQEVGAPSNCLTPE
                     ETPGFLEATVRSAARTENLWGVTWWCSHDVSRELADYPELEYSLGLLENGGAVKPIGR
                     RFAEIIPELRTRGVAPARTTGIVVDVDDADVPVSRAALSPGGAVFQAWVDACETGVDA
                     SFVTSKDAADAAVLASRGITELVRPDLSTGVGVYSSNNTVVS"
     gene            9818..11119
                     /locus_tag="VN041_16320"
     CDS             9818..11119
                     /locus_tag="VN041_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30647.1"
                     /translation="MKIRSTLAVGLTAATVLALTACTGGGGTPDAGGSGPIDTSGELS
                     GTIQFQTWSLKNEKFTPYFTSLIKAFEKEHPKVKVDWIDQPGDGYQDKILSQANSNTL
                     PDVLNLPPDIAFPLVKANKLLDLAKADPKLKTVYNAGGWEAYSKFSGVEGTYGLPWYL
                     GSDESWWNLEQLQPYGVTEADLPTTTDGWLDLAKKVAQESNGKVQLVSSMPGLDTFVA
                     ADIPIMKDDGTFVFNTPEAVDIVQKYADAYKAGAMPPESLTGSYGGNAEMYLQGKVAY
                     STGGSGFAGDLRTKAPTLLEHTVATQRPGTPPLFVQGVNVSADSKNKAAALAFAEFVT
                     NEKNQVDFAKIAVGFAPGTAAGAEDVVKAVESSVDPAQAKAMKVMFDALPKAKSTPFQ
                     WTGAMTDYLNQQMALAIRGDATAKDTLDKIVKYANDNRIDK"
     gene            11174..12061
                     /locus_tag="VN041_16325"
     CDS             11174..12061
                     /locus_tag="VN041_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30648.1"
                     /translation="MRSHRWFTPWLLLAPAVIWVLVFSLWPFLNTVVLSFTDARPLQP
                     VKGVGFAQYERLLTDEQFRYALITCLIYVIVCVPLLTVLPLLLALLVEKNIPGIALFR
                     TTYYFPVIASVVVVALIWGWLFDSRGIINEGLQWMGLTDQPVAFLVDRWWMLAVSILL
                     TVWKGLGYYMVVYLAALGNVGKDLHEAAMLDGAGSFRRFVSVTIPSVRGAMLLISVLV
                     AVSAMRVFTELDVLSNGTGGPGGYNMSIVMLIRQVGSGLSGNLGYAAAISVALFLLTL
                     IPLVAIALMNREKKAKVPA"
     gene            12058..12987
                     /locus_tag="VN041_16330"
     CDS             12058..12987
                     /locus_tag="VN041_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30649.1"
                     /translation="MSVIEDRPAASAPQPAEQRIRPDRRFRRKGSGDFTKPTVGGLIG
                     RYALLTLVLILFIGPFVWQLATSFKGPADDLYTFPPNLIPTDPTIQNYIKVTDLIPVY
                     TYAWHSLLVATGAVVTNVVLSVFGGYALACMKFRGKFIALGILVSTMLLPGEVTVTAN
                     LLTINSLGLANSLWGVFLPGAIGAMNVLLIATACRMIPQDVLDAATVDGATTWQRIRH
                     IVWPNVRGMASVVAIFTFIGQWDDYLWPLMVLTDPNNYTLTVGMAYLNGNFSPDPRLI
                     AAGTMVALIPIILLFSVMQRFFFQGVQEGAVKG"
     gene            12984..>13419
                     /locus_tag="VN041_16335"
     CDS             12984..>13419
                     /locus_tag="VN041_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-mannosidase"
                     /protein_id="HWU30650.1"
                     /translation="MTSATLSPAEGPADPPSSRPRAGVLGGRVHAFRFDPVAGVQRMP
                     LPGGFEVAPDAVLHWAFYADGPDATLAPHAALAVTIDVAFERGPGSAEERLSQHPDAR
                     DRYGVPLTAAAQFDAAWSMPEQWNADSVSLEAWAGCAARVEVV"
BASE COUNT         2050 a         4708 c         4539 g         2122 t
ORIGIN      
        1 aggacatcgc cgacctcgcg gcctactaca gccagcacgg cgcagccgga ccgcatcggc
       61 tacgtctcgg acgccgacgg ctccaccgtc gccatggccg cggcccttcg cctgctgcgc
      121 ctcgccgccg cgggtgcgca actgcccggc gacgtgatcg tgaccaccca catcgccacg
      181 cacgtcagca tcaccccgcg cgagcccgcc gacttcatgg gcatgcccgt gtcatcggag
      241 acgatgaacc tgcacgaggt ggatgccgcg atggacgcga tcctgtccat cgacgcctcc
      301 aagggcaatc ggctcatcaa ccacccgggc gtggccatct caccgaccgc gatggccggt
      361 tacctgctgc ccgtggcgcc agggctgctg accatcctgg agcaggcgac gggggcaccc
      421 gctcgcacct tcccgctcgc cctgcaggac atcacgccgt actccaacgg gtaccggcac
      481 ttcaactcga tcatgcagcc gaccgtcgcc acgagcgctc cggtcgtcgg cgtggcattg
      541 acggccgcgg ccgtcgtccc cggttcatcc acgggcgcca gctacgagcc cgcgctgcag
      601 gaggcgaccc gattcgtcgt cgagaccgcc aagcagttca cccaggaccg cgtgtccttc
      661 ttcgacatcg acgagtacga ccgtctcgtc gcccagtacg gggacctcac ccggttccag
      721 agctgacccg cccgatcacg ggcatccgcc ggcgatgaag ccgacgtccg aaagtcatca
      781 gaaagtctgc acatgacctc ctctccctcc acggagcagc tgcctcccaa gacgcacccc
      841 cgcgccttcg aaccgatcac cctcatcgtc accgtcgtgc tgagcgtgct cggcgccgtc
      901 atcggcgtgc acctgatcac cacgctcggc atctcggcga acacgagtgt catcggcgcc
      961 gtgatcgcca tgctgctcgg ccgcatcacg ttcttcgggt tcgcgaagtt ccgcagcaag
     1021 caccgtcaga acctcatcca gtcggcggtg tccgctgcga cgttcgccgc cgccaacggc
     1081 ctgctcgcgc ccatcgcgat cccgtgggcg atgggcaagc ccgagctggt gtggcccatg
     1141 ctgctcggtg catcgctcgg cgtgctggtc gacgtgttcg tgctgttcaa ggccttcggc
     1201 tcgaagttcc tgccggcgtc cgccgcgtgg ccccccggcg tcgccgcggc cgagaccatc
     1261 ctcgcgggca aggagggcgg ccgtcgtgcg ctcatcctcg tcggcggcgg cgtgatcggc
     1321 gtgatcggcg tgatcggctc gctgttcgcg ctgcccatgt cggcagccgg cgtcgcgctc
     1381 atcggcaaca tctgggccct gctgatgttc gcggtgggcc tgctcatctc gcagtacgca
     1441 cccgtgctct tccagatcaa cctcggcgcg ctgtacatcc cgcacggcgt gatgatcggt
     1501 gcgggcgtgg tcgccctgct gcaggtgatg tggatcatct tcggcgcgcg gaccaagcag
     1561 cagcaggcgc aggaggcgcg ccacaccgag gcgatcaaac tcgacgagac cctcgactac
     1621 acgatctccc gcaaggggct ggggcgggcg ctcggactgg gctacgtcgc attcgtcgtc
     1681 ggtgccctcg tgatcgcgct ggtcggtggc gtggcatcag acctgtcgat tcccgcgctg
     1741 atcgggttcg tgctgttcgc cggcttcgcg gccatcgtgc acgaactgat cgtgggcctg
     1801 gcggcgatgc acgcgggatg gttcccggct ttcgccgtca cgctgatctt cctgatcctg
     1861 ggcctgttcc tgcagatgcc gcaggtgccg ctgctggtcc tggtggcgta ctgctccgcc
     1921 accggtcccg ctttcgccga catgggctac gactacaagg ccggatggat cctgcgccgc
     1981 gagggccggc cgtggaaggc gtacgagctg cagggccgtc gcgagcaact gaaggcgtcg
     2041 ctggtcgcga tcgtgatcgc catcggcatc gtcgcgatct cctggcaggg tctcttcgcc
     2101 gacgggaaga tcccgccgtc ctctaaggtg ttcgcggaca ccctcgcggt gggcatctcc
     2161 gacccgcacg tggcgctgac catggcgctc tgggccatcc ccggcgcgct catccagctg
     2221 atcggcggct ccaagcgcca gatgggcgtg ctgctggcga ccggcctgct ggtcgccacg
     2281 gcgaacgccg gctggctggt gctcgcggcc ctgctgttcc gcgtgatctg gctgaagtac
     2341 cgcggcgcga agggcgagca ggagatcgcg ctgatcggcg ccggcgtcat cgccgcggac
     2401 tccctgatga gcgtcggcaa ggtcatcaag ttctgatcaa cggcaccccg cggccggcct
     2461 gctgagaaca ggagaaccgc ccggaacagg acagaatcgc acgaggttct cctgtttcga
     2521 atggttctcc tgttctggga agcgcgatcg ggtgggcgcg tcagcgggcc agcggatgct
     2581 ccgcccccag cgcctcgagc ttgcgcgggt tggccatcga gaacacccgg gagatccgcc
     2641 cgtcctcgac ctcgaagctc accgccgcca tcagcacgcc gccgacctcg atccgcgccg
     2701 cgagccggcc gttcacggtc atcgcggaca cccggtaggc gatgtcgagc ttggcgatgc
     2761 tgccgcgcag gaatgccacc accctgtccg caccgaagag cgggcgacgg gcggcatggc
     2821 gcaccaggcc gccgctgtcg gtggtcgaca ccacgtccgg cgccagcacg tccatcagcc
     2881 cctgcatgtc gcccgaatcg agcgcggcga tgaagcgccg caccacgcgg tcgtgctcgg
     2941 aggcgtccac ctccacgcgc ggacgtcgcg cggccacgtg gtctcgggcc cggtgcgcga
     3001 tctggcgcac cgtggctgcg gtcttgtcca cggcatccgc gatctcgtcg aagggcacgt
     3061 cgaagacctc gcgcagcacg aacaccgctc gctcggtcgg ggtgagcgtc tcgagcacgg
     3121 tgagcatcgc gagcgagacg ctctccgcga gcaccacgtc gtcggcgacg tcgggggagg
     3181 tcagcagcgg ctcgggcagc cacggcccca cgtactcctc acgccggcgc gacaccgtcc
     3241 gcagctggtt cagcgactgc cgtgtgacga tccgcaccag gtacgcccgc ggatcgtgca
     3301 cgcggccgcg gtcgacatcc gcccaccgca gccacgactc ctgcagcacg tcctcggcat
     3361 ccgccgcaga gccgaggatc tcgtaggcga cggtgaacag cagcccgcgg tggacgacga
     3421 acgggtcctc ggatgccgga tcggtggagc cgctcatgag tgcgacgcta cccccgcggg
     3481 cgtcgccgtg gccagcggcg ccagcccgca ggcatccgcg agtccgtcgg agtggacgcc
     3541 gagggcgacg ttcatgcggg ccgtggcgtt gaggtacccg accttcgccg ccagctcgag
     3601 cagggcgggt gcgccgagct cggcgagcag cgcggcggag agctcgtcgg tgaccgcagg
     3661 tggggtctgg ctcatcgcct cggcgtactc catcacgcgg cgctccagcg gggtgaaccg
     3721 cgtcgattcg cgccagatcg gaacctcgcg cgccttctcc tcggcgaggc cgtggtcgcg
     3781 cgacatgaag tagtgcagat ccaggcagaa gctgcagccc accgtcgcgg cgcacgccat
     3841 gctggcgagc gtgcccaggc tctgatcgag ctcgcgccac ttctcggtgc ggccgccgaa
     3901 cttcatcatg tccatcatca ccgggcggtg ctgccacatc accccgaggg agtccggcac
     3961 cttgccgagc atcttcttcg cggcggcctt caccaccgcg ccgtacaggc cggtcaccgg
     4021 gatggcggga atgcgggtct cgttcatgat cgctgctcct cagccggcac gtctctcgtg
     4081 ccgtcggcat gaagacaccg cccgtcccgg aagtgtgaca tccgggacgg gcggtgcggt
     4141 ctgatccgtt cgggaggaga actccggctc gggaggagga atccgaggga cgcctcctcc
     4201 cgtccgtgag atctcctccc gagagagcgg atgctgcgcc tcaggcgcgc gcgatgcgga
     4261 gcgtgcgcac ctcgaacggc ttgagcgtca gctcaccgcc ggtgcgcggg gagtccagct
     4321 catcctcgat caggctcacc tcgcggatgc cggtgtgctc gaagccgacg gtcagcgtgc
     4381 ccgtcgtgcg gcgtccgagc gactcgtaca cgcggacgat gacgtcgccc gagcggtcat
     4441 cggcgagctt cacgctggac acgacgatcc cggagccggt cgccgagacg agcggctcca
     4501 cctcgttcgc gccgcgcacc acgtgcgcgg gagcgttcag gcggatgcct tcggccgtcg
     4561 cgatctcggc atccgcgccg atcaccagac cgacctcgat ctcgtgccgg ccgtggtcgg
     4621 tgtccgggtc ggggaagcgc ggcgcgcgca gcagcgacag gcgcacggtg gtggtgacgt
     4681 cgtcgccgtc cacctcgcgg gaggtgtcgt agccgtagat cgagtcgttc acgagcgcga
     4741 cgccgaagcc gtcgtcctcc tgggcgagca cgaagcggtg catggaggtc tcgaacttgg
     4801 cggcctccca gctggtgttc acgtgcgtga cgcggcgctg gaagccgaac tgcgtctcgg
     4861 ccagggtctc ccgggcgaac acgtcgagcg ggaacgcgag cttgagcagc ttctcggtct
     4921 catgccagtc gatgtcgttc tgcagcagca cggtgcgcga gcccgggcgc agcgtgatcg
     4981 tctgcgtcag gcgcgattcg gagaatgcgc gctcgaccac cacgacggcg gcgccgtcga
     5041 ccacggatgc cgagatcgac gagaccgccg tgaggtcgtc gacgcggttc ttgtagtacc
     5101 ggtcgatgtc ccatgcgtcc cacatgttcg ggaagtcctg gtgcagctgg aacaggttcg
     5161 ccggctttcc ggcggggatc gcctcgcggc cggtggcctt gtcgacggcc gaggtgatca
     5221 ggccctcggc cgagacgatg acggcgacga ggtcgttgga gagcacgaag ccgtcgggcg
     5281 actccgtgag gctggcggat gctgtgctca ggacgaccgg tgccgcgccc agcgcccgtc
     5341 cgccgccgac cgaggtcggg gtgaacacca gctcgcggtc gccctcgccg gccagcgcct
     5401 gtcgggccgt ggacgacagc ttctcggcat ccgacagcac tcccgagagg acctccacgg
     5461 cctcgcggtg cacccatgcg atcgaggtgc cgggcaggat gtcgtggaac tcgtgcagca
     5521 gcacggtctg ccacagccgg tccagctcgt cgtgggggta ctcgacaccc cgctgcacgg
     5581 tcgcggtcgc ggcccacagc tcggcctcga tcagggcctg ctcggcccag cggtgcagcg
     5641 ccttggtggc gtgctggctg gtgagcgtgc cgcggtgcag ctcgaggtac agctcgccga
     5701 cccagacggc ggggtcggtc agctcggccc tggcgtcgtc gaagaagtcg tccggatgct
     5761 tccactcgac ctgcgcggcg ccttccagcg acgccatacg ggccgccttg cctgtcatct
     5821 cgcgggtcgt gccaccgccg ccgtcgcccc agccgaccgg tgcgatcgac atggtggcct
     5881 tgcggttctc cttgaactgc cggctggcct tcgcgacctc cgagccgctc agctgcgagt
     5941 tgtaggtgtc catcggcggg aagtgcgtga acatccgcga gccgtcgatg ccctcccaca
     6001 ggaacgagtg gtgcgggaag acgttgcgct ggttccagga gatcttctgc gtgaagaacc
     6061 actcgaaccc ggcccggcgc atcagctgcg gcagcgcggg cgagtaaccg aagctgtcgg
     6121 gcagccacac gcccttcgaa cggatgccga actcgcgctc gaagaagcgc tggccgtgcg
     6181 agaactggcg caccagcgac tcgccggtcg gcatgaccgt gtcggactcc acccacatgc
     6241 cgcccagggg caggaagcgt ccggccgcga ccgcgtcctt cacccgtgcg aacacctcgg
     6301 ggcggtgctc cttgatccag gcgtactgct gcgcgctgga catgccgtag aggaaatcgg
     6361 gctgctcgtc gatcagcgtc gtcatcgacg aggtggtgcg ggcgaccttg cggatggtct
     6421 cacgcaccgg ccacagccac gccgagtcga tgtgcgcgtg accgatcgcg gcgatgcggt
     6481 gcgcactggc atccgccggt gaggcgagca cgtcggccag ccgggcgcgg gcatccggcg
     6541 ccgtctcgac gatgtgctgc aggtcgagca catcgagggc gtcgtcgaga gcctgcagga
     6601 tgcgcatgcg gcgcgggctg gtctcgggca gctccgcctg cagctcgatc aggacctcga
     6661 ggtcgagggc gagctcgaac accgcctcct cgaacacggc gagatccatg tgccgcacgc
     6721 ggtacagcgg ctcgggggag gaggtgcgga tgtcgccttc ctgcgtcggc aggaacgggt
     6781 ggtagtccag cagcaccggg ttggcggcgc cctccaggta gaactccacg gctccgcccg
     6841 gctcggcctc gacagccacc cactggttgc gcggattgat gctcttcagc ggcgtgccgt
     6901 cggggcggta ggcgagcgcc tcgcactgga aaccgggcat gttgatgtcg aagccgaggt
     6961 cgatgagggc ctcgatccgc cggccggacc actccgcagg aaccgacccg cgcacgcgga
     7021 accaggtggt gctccaggcg ggcccccaca tgctgcctgc ggtgaacggc tcgaactcga
     7081 gcgcgaggcc ctccaccggg gcgatcggct cgccgggcag ctggtgcgcc gcgatctcga
     7141 gcggcacccg ggaggagtgg atcgcggggc gcacgcgctc ggtgagcacg cggttcacac
     7201 ggccgacggt gagggaggtc tcttcgtgca cgggggctcc ttgcagatgg aagaatgagg
     7261 gcgatcggct gcggggttca ctgggacggc cgatactaaa acgatctagt acggtgagtc
     7321 tatgtcacgg tccaagcgtg tcaccatcgc ggatatcgcg cgacaggcgg gagtctcgcc
     7381 cggtgcggtg tccttcgccc tgaacgggcg ccccggggtg agcgaggaga cgcggcagcg
     7441 gatcctggcg atcgcagacg agcaccagtg gcggcccagc tccaccgccc gcgcactggt
     7501 cggcgcgagg gcgggagtcg tcggcttcgc ggtgaaccgc cccgcccgca ccctcggcac
     7561 cgaggcgttc ttcaccgacc tgatcgccgg tgtgcagtcg gcgctcgccg cccgtcgcat
     7621 cggcatgcag ctggtcgtcg tgcccagcat ggatgaggag atggacacgt accggcggtg
     7681 gcgccggtcc aaccaggtcg acggcgtcct cgtgctcgac ccgcgcgtcg acgacccgcg
     7741 accagggctg ctgcgctcgc tcgggctgcc ctcgatcacg atcggcggcc agccgtcccc
     7801 ggacgacatg cacgccactc tgtggctgga cgacgcggac gccgcccgca cggtcttcgg
     7861 gtacctggcg ggcctcggcc atcgcagcat cgtctacatc tccggccccg ctgagcacga
     7921 gcacgtccgg ctgcgcagca gcgtcctggc cgccatggcc gagcgggaca tcgtcggcga
     7981 ggtcatccag accgactact cgccggcgca ggtgtccacg gtcatgcgca cgctgctcag
     8041 ccgagccgaa cggccgacgg cggtcgtctt cgacaacgac atgatggcga tcgcggggct
     8101 ccgcatcacc caggagatgg gcgtcgccgt gcctgccggg ctctccctgg catccttcga
     8161 cgactcgatc gtgaccggcc tcgtccgtcc gtcgatcacc tgcctcaccc gcgacacgtt
     8221 cacgctcggg gagaccgcgg ccgacttcct gatcgagcag ctggattccg ccgaggtgct
     8281 gccggaccgc accgggccgc agccgaggct gacggtgcgc gagagcacgg cgccgccgcg
     8341 ctcctgagcg ccccgctgct gagctccgca ctcaggagac gacggtgttg ttggacgagt
     8401 agacgcccac ccccgtcgaa aggtccggcc gcaccagctc ggtgatgccg cgggacgcca
     8461 gcaccgcggc atccgcggca tccttcgacg tgacgaacga tgcgtccacg cccgtctcgc
     8521 acgcgtcgac ccaggcctgg aagaccgcgc cgcccgggct gagcgcggcg cggctcaccg
     8581 gcacgtcggc gtcgtcgacg tcgaccacga tgccggtcgt tcgcgccggt gcgacgccgc
     8641 gtgtacgcag ctcggggatg atctcggcga accgccggcc gatcggcttg acagccccgc
     8701 cgttctccag cagacccagc gagtactcca gctcggggta gtccgcgagc tctcggctca
     8761 catcgtgcga gcaccaccag gtgacgcccc acaggttctc ggtgcgagca gcggagcgca
     8821 cggtcgcctc caggaagccc ggcgtctcct ctggggtgag gcagttcgac ggggcgccga
     8881 cctcctgcag ccagatcggc ctggcgggat cggtcgcgaa ggcgcgggag agctcgatca
     8941 tgtactcggc atgacggtcg gatgccgcgg agcggccgcc gtagcgctgc gccgtgccgt
     9001 tgaagatcca ggagtgcacg gtggtcatgg cgcccaggcg cgaggcgagc gcgggggtga
     9061 agccgtgccc gtccatgtac caggcggcgt cgtactcgct gtgcaggtga tcgcggccag
     9121 gggccgcgcg ctcagcggca tccagcagcg aggcgatcca gttcgccgcc tcggcctcgg
     9181 tgacgggcca ctgatgcggg tgcgtctcgg cggagaactg gttggtctcg ttgccgagcg
     9241 tgaacccgag gaagttgccg gcgtcgttca gtgccgcgcc gagcgtctcc accagggcga
     9301 cctggccgct gagcgcgtcc gggtgcgtga acatgttctt gtcgtgccag gtgaacagcc
     9361 acgaggggac gaagtcgaag ctcgacaggt ggccctggat cacgtcgacc ccggcgtcca
     9421 gcccgaactc cgccgcgacg tcgaccacgg cgcgcacatc cgcgaccgcc ttcccgcgga
     9481 tcagggtgcg attcggctgc agcacggtcc agagcgggaa gatccgcacg tggtcgagcc
     9541 cgagcgatgc cagcccctcg aagtcgcggc gcacgtcgtc gaggtccagg gacagccacg
     9601 agtgcatcca gtccttcgac ggggtgtagt tggcgccgaa acgcagcggt gcgtcggcgg
     9661 acgtgggtgc tggcatgggg gaagccttcc tgagcgcgtc acccttcgag gtggacgtcg
     9721 tcgctctcct gtgcaatgct atggtcacta tagcggttta gtaatggatg cgaaccccag
     9781 cgggtgcgca gccggacccc ttcgaaggag aacgtccatg aagatccgat cgactctcgc
     9841 cgtcggcctc accgcggcga cggtgctcgc gctgacggcc tgcaccggag gcggcggcac
     9901 accggatgcc ggcggttccg gccccatcga cacgtcggga gagctcagcg gcaccatcca
     9961 gttccagacc tggtcgctca agaacgagaa gttcacgccg tacttcacct cgctcatcaa
    10021 ggcgttcgag aaggagcacc ccaaggtcaa ggtggactgg atcgaccagc ccggcgacgg
    10081 ctaccaggac aagatcctca gccaggccaa ctcgaacacc ctgcccgacg tgctcaacct
    10141 gccgccggac atcgcgttcc cgctggtcaa ggcgaacaag ctcctcgacc tcgccaaggc
    10201 cgatcccaag ctgaagaccg tctacaacgc gggcggctgg gaggcctaca gcaagttcag
    10261 cggcgtcgag ggcacctacg gtctgccctg gtacctcggc agcgacgagt cgtggtggaa
    10321 cctggagcag ctgcagccct acggcgtgac cgaggccgac ctgcccacca ccaccgacgg
    10381 ctggctcgac ctcgccaaga aggtcgccca ggagtcgaac ggaaaggtgc agctggtctc
    10441 gtccatgccc ggtctggaca cgttcgtcgc cgccgacatc ccgatcatga aggacgacgg
    10501 gacgttcgtc ttcaacaccc ccgaagccgt cgacatcgtg cagaagtacg cggacgccta
    10561 caaggccggc gccatgccgc ctgagtcgct gacgggcagc tacggcggca acgccgagat
    10621 gtacctgcag ggcaaggtcg cctactcgac cggtggttcc ggcttcgccg gcgacctgcg
    10681 caccaaggca cccaccctgc tcgagcacac cgtcgccact cagcgccccg gcaccccgcc
    10741 gctgttcgtc cagggcgtga acgtctccgc cgactcgaag aacaaggccg ccgcactcgc
    10801 gttcgcggag ttcgtcacca acgagaagaa ccaggtcgac ttcgccaaga tcgccgtcgg
    10861 cttcgccccc ggcaccgccg cgggtgcgga ggacgtcgtc aaggccgtcg agagcagtgt
    10921 cgacccggcg caggcgaagg ccatgaaggt catgttcgac gcactgccga aggccaagtc
    10981 gaccccgttc cagtggaccg gggcgatgac cgactacctc aaccagcaga tggcgctcgc
    11041 catccgcggc gacgcgacgg cgaaggacac gctcgacaag atcgtgaagt acgccaacga
    11101 caaccgcatc gacaagtgac cggaccccga gtccgatgat cgagaacacc agcaccccga
    11161 agaggtacgc gccatgagat cgcacagatg gttcaccccc tggctgctcc tggcccccgc
    11221 cgtcatctgg gtgctggtgt tctcgctctg gccgttcctg aacaccgtcg tgctgagctt
    11281 caccgacgcg cgacccctgc agccggtcaa gggggtcggc ttcgcgcagt acgagcggct
    11341 gctgaccgat gagcagttcc ggtacgcgct gatcacctgc ctgatctacg tcatcgtctg
    11401 cgtgccgctg ctgaccgtgc tgcccctgct gctggctctt ctcgtggaga agaacatccc
    11461 cggcatcgcc ctcttccgca ccacgtacta cttcccggtg atcgcgtcgg tcgtggtggt
    11521 cgcgctgatc tggggatggc tgttcgacag tcgcggcatc atcaacgagg ggctgcagtg
    11581 gatgggcctc acggatcagc cggtcgcgtt cctggtcgac cggtggtgga tgctggccgt
    11641 cagcatcctg ctgaccgtgt ggaaaggtct cggctactac atggtcgtgt acctcgcggc
    11701 cctcggcaac gtcggcaagg acctgcacga ggccgccatg ctcgacggcg caggcagctt
    11761 ccgccgattc gtcagcgtca ccatccccag cgtccgcggg gcgatgctgc tgatctcggt
    11821 gctcgtcgcc gtgtcggcca tgcgcgtctt caccgagctg gatgtgctct ccaacggcac
    11881 cggcgggccc ggcggctaca acatgtcgat cgtcatgctg atccggcagg tcggatcggg
    11941 cctgagcggc aacctcggct acgccgccgc gatcagcgtc gcgctgttcc tgctgacgtt
    12001 gatcccgctg gtcgccatcg ccctgatgaa ccgtgagaag aaggcgaagg tgccggcatg
    12061 agcgtcatcg aagaccgccc agcggcatcc gctccgcagc ccgctgagca gcggatccgc
    12121 cccgaccgcc ggttccgccg caagggctca ggcgacttca ccaagccgac cgtcggcggc
    12181 ctgatcggcc gttacgcgct gctgacgctg gtgctgatcc tgttcatcgg cccgttcgtg
    12241 tggcagctcg cgacctcgtt caagggtccc gccgacgacc tctacacctt cccgccgaac
    12301 ctcatcccca ccgaccccac gatccagaac tacatcaagg tcaccgacct gatcccggtc
    12361 tacacctacg cctggcactc cctgctggtc gccaccggcg cggtcgtcac caacgtcgtg
    12421 ctctcggtgt tcggcggcta tgcgctggcg tgcatgaagt tccgcggcaa gttcatcgcc
    12481 ctcggcatcc tggtctccac catgctgctg ccgggagagg tgacggtcac cgcgaacctg
    12541 ctcacgatca actcgctggg tctggcgaac tcgttgtggg gcgtgttcct gcccggcgcg
    12601 atcggcgcga tgaacgtgct gctgatcgcg accgcgtgcc gcatgatccc gcaggacgtg
    12661 ctcgacgccg caaccgtcga cggcgccacc acctggcagc ggatccgtca catcgtgtgg
    12721 ccgaacgtgc gcggcatggc gagcgtcgtg gcgatcttca ccttcatcgg ccagtgggac
    12781 gactacctgt ggccgctgat ggtgctcacc gacccgaaca actacacgct cacggtcggc
    12841 atggcttacc tgaacggcaa cttctcgccc gacccgcgtc tgatcgcggc gggcaccatg
    12901 gtggcgctga tcccgatcat cctgctgttc tcggtgatgc agcgcttctt cttccagggc
    12961 gtgcaagagg gagccgtcaa gggatgacgt cagcgacgct gagccccgcc gaaggccccg
    13021 cggatccgcc ctcgtcccgt ccccgggccg gcgtgctggg cgggcgcgtg cacgcgttcc
    13081 ggttcgatcc cgtcgctggg gtgcagcgga tgccactgcc gggcggattc gaggtggcgc
    13141 cggatgccgt gctgcactgg gcgttctacg ccgacggccc ggacgcgacg ctcgccccgc
    13201 acgcggcgct cgccgttacg atcgacgtgg cgttcgagcg tgggcccggc agcgcggagg
    13261 agcggctgag tcagcacccg gatgcccgtg atcgatacgg cgtgccgctg acagcggctg
    13321 cgcagttcga tgcggcctgg tcgatgcccg agcagtggaa cgccgactcg gtgtcgctcg
    13381 aggcgtgggc cggctgcgcg gcgcgggtcg aggtcgtgc
//
LOCUS       DATMSX010000507         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_111885,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000507 DATMSX010000000
VERSION     DATMSX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111885"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1318
                     /locus_tag="VN041_16340"
     CDS             263..1318
                     /locus_tag="VN041_16340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWU30651.1"
                     /translation="MTSAALSVLDLVPVRSGVDGSTQTSAQAVAASMALAQRADELGY
                     RRYWFAEHHNMPSVASTTPPVLIAAAASRTSRIRVGSGGVMLPNHAPLVVAEQFAALE
                     AIAPGRIDLGIGRAPGSDPVITQLLRQSGTSGDVERFPSHVQDIALMLRHEGATVRFT
                     SGGEYTVRATPMASDAPEIWLLGSSDYSAQLAASLGLPYVFANHFSGQGLQRALDLYR
                     SSFQPSSVLETPRTFLTANVVAAPTADEAEARALPQLRMMTRLRRNQPLTPLETVEQA
                     QRGLAEADAGEQTLVDGIRANWFIGEAGEVRTTLDAFAAQYGVDEIMISPVAGSFESE
                     PLDATPGRVQTLELLAR"
     gene            complement(1323..2267)
                     /gene="pheA"
                     /locus_tag="VN041_16345"
     CDS             complement(1323..2267)
                     /gene="pheA"
                     /locus_tag="VN041_16345"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWU30652.1"
                     /translation="MTERRTYSYLGPAGTFTEAALDQVPEARGHEWKPVHNVGEALAD
                     VLSGVSDAAMIAIENSIEGGVSTTLDALATNPGLRIIGEYLVKVNFVLVGVPGTKLED
                     VQVIAAHPVAYAQCHGWLGAHLPRHAHVPAASNVASALGILDGTLPAQAAIAAPGIVQ
                     HQDVEVLAEQIGDNDNAVTRFVLVSRTTAAGAPTGADKTSLIVELPDDRPGALLEMLE
                     QFSTRGINLSLIESRPIGDELGRYRFVLDADGHIQDERMADALLGIRRFSPRVVFLGS
                     YPRADRRIVQYPDRYADEIFVEARDWLRGLIAGEPDET"
     gene            2500..4143
                     /gene="pgm"
                     /locus_tag="VN041_16350"
     CDS             2500..4143
                     /gene="pgm"
                     /locus_tag="VN041_16350"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HWU30653.1"
                     /translation="MTSRAGLPAEEKDLIDVDELIAAYYDRHPDPAIATQRVVFGTSG
                     HRGSALSSSFNEDHILATTQAIVDYRNGQGITGPLFLGRDTHALSLPAERSAIEVLIA
                     NGVDVRVDSRDAWVPTPALSHAILTYNRGRAADDSGRADGIVVTPSHNPPRDGGFKYN
                     PPHGGPADTDATGWIAQRANELIEAKLDGVKRESFADVDWDAITGYDFRDAYVRDLPN
                     IIDIDAIKRAGVRIGADPLGGASVEYWQLIKDMHGLDLTVVNPEVDPTWRFMTLDWDE
                     KIRMDPSSPSAMASLVAKKGDFDVLTGNDADADRHGIVTPDAGLMNPNHYLAVAIDYL
                     FSHRASWPRDAAIGKTLVSSMIIDRVAESLGRRLLEVPVGFKWFVPGLLDGSVAFGGE
                     ESAGASFLRHDGSVWSTDKDGILLCLLAAEIIAVTGKSPSERYAELEAAFGASAYQRV
                     DAPATPEQKATLGKLAPDAVTATDLAGEEITAKLSHAPGNGAAIGGLKVQTEHAWFAA
                     RPSGTEDVYKLYAESLLGADHLAQVQEEARAVVSAALGG"
BASE COUNT          664 a         1595 c         1524 g          656 t
ORIGIN      
        1 acgggcacgc cgccgcgcgg caggccgagc acgacgcagt cctcatgggc gcgatgggtc
       61 agctcgcggg cgagctctcg gcccgcgtcg gctctgtcct cgaagttcca catggcgtca
      121 cctgaccccc ggattacgcc gggcggatgc ggtgcgtcaa gcccctggcg cagggggctc
      181 cgccgtgtgc cacgcgatcg gcggacgtgg ttgagaccga gagcgcggaa tgccacggca
      241 tccgcttcgg ttggccctga tgatgacttc tgcagcgctc tccgtcctcg atctcgttcc
      301 cgtccgctct ggcgtcgacg gctccaccca gaccagcgcg caggcggtgg ccgcatcgat
      361 ggcgctcgcc cagcgcgccg atgagctcgg ctaccgacgc tactggttcg cggagcacca
      421 caacatgccg tccgtggcat ccacgactcc cccggtgctg atcgcggccg cggcgtcgcg
      481 cacgtctcgg atccgggtcg gctcaggcgg cgtcatgctg cccaaccacg cgcctctggt
      541 ggtggccgag cagttcgccg cgctcgaggc gatcgcgccc ggacgcatcg acctcggcat
      601 cggccgcgcc ccgggcagcg atccggtgat cacgcagctg ctgcgccaat cgggcacctc
      661 cggtgacgtg gagcgcttcc cgtcgcacgt gcaggacatc gcgctgatgc tgcgtcacga
      721 gggtgcgacc gtgcgcttca ccagcggcgg cgagtacacc gtgcgtgcga cgccgatggc
      781 atccgatgcg cccgagatct ggctcctggg ttcgagcgac tactcggccc agctcgcggc
      841 atccctcggc ctgccctacg tgttcgcgaa ccacttctcc ggtcagggac tgcagcgcgc
      901 gctcgacctc taccgctcgt cgttccagcc gtcttccgtg ctcgagacac cgcggacgtt
      961 cctgaccgcg aacgtcgtgg ccgcgccgac cgccgatgag gcggaggcgc gcgcactgcc
     1021 ccagctgcgc atgatgacgc ggctgcgccg caaccagccg ctcaccccgc tggaaacggt
     1081 cgagcaggcg cagcgcggcc tggccgaggc ggatgccggc gagcagacgc tcgtcgacgg
     1141 catccgcgcc aactggttca tcggcgaagc cggtgaggtg cgcaccacgc tggacgcctt
     1201 cgccgcgcag tacggcgtgg acgagatcat gatctctccg gtggccggat cgttcgagtc
     1261 cgaaccgctg gacgcgacac ccggccgcgt gcagaccctg gagctgctcg cgcgctgagc
     1321 gcctacgtct cgtcgggctc gccggcgatc aggccgcgca gccagtcgcg ggcctcgacg
     1381 aagatctcgt cggcgtaccg gtcggggtac tgcacgatgc ggcggtcggc gcgcggatag
     1441 gagccgagga acaccacgcg ggggctgaac cggcggatgc cgagcagcgc gtcggccatg
     1501 cgctcgtcct ggatgtgccc gtcggcgtcg agcacgaagc ggtaccgccc cagctcgtcg
     1561 ccgatgggcc gcgactcgat cagcgacagg ttgatgccgc gggtggagaa ctgctccagc
     1621 atctccagca gggcgcccgg tcggtcgtcg ggcagctcga cgatcagcga ggtcttgtcg
     1681 gctccggtcg gcgccccggc ggcggtcgtg cgagagacca gcacgaagcg ggtcacggcg
     1741 ttgtcgttgt caccgatctg ctcggccagc acctcgacgt cctggtgctg cacgatgccg
     1801 ggcgccgcga tcgcggcctg cgccggcagc gttccgtcca ggatgcccag ggcggatgcc
     1861 acattgctgg ccgccggaac atgagcgtgc ctggggaggt gcgctccgag ccagccgtgg
     1921 cactgcgcgt aggcgacggg gtgcgccgcg atgacctgca catcctccag cttcgtcccg
     1981 ggcacaccga ccagcacgaa gttcaccttg acgaggtact ccccgatgat ccgcagaccc
     2041 gggttcgtcg ccagggcgtc caacgtcgtg gagacgccgc cctcgatcga gttctcgatc
     2101 gcgatcatgg ccgcatccga cacgccggag agcacgtcgg cgagcgcctc gcccacgttg
     2161 tgcaccggct tccactcgtg accgcgggcc tcgggcacct ggtccagggc cgcttcggtg
     2221 aaggttccgg cagggcccag gtagctgtac gtgcggcgtt cggtcacccg gcaagcctag
     2281 tcccgggaat ccgccgggtt acggccggcg atccttgagg gctgcgcgtg cgcttcgcgc
     2341 ggagatgtac gcctgccgcg gacgaaatcc ggcatccgat ccgcgcgaag cgcacattcc
     2401 cgcgtgaacc gtacggcaca ggcgaccctg aggtccgccc gactgcatcc atccgactcc
     2461 gaagatgcgc gacggccacc cgcggggcag actatccaca tgacgagccg tgcaggcctc
     2521 cccgcggagg aaaaggacct catcgatgtc gacgagctga tcgccgcgta ctacgaccgg
     2581 catcctgatc cggcgatcgc gacgcagcgc gtcgtgttcg ggaccagcgg ccatcgcggc
     2641 tcggcgctgt cgtccagctt caatgaggac cacatcctcg cgaccacgca ggcgatcgtc
     2701 gactaccgca acgggcaggg catcacgggt ccgttgttcc tcggccgcga cacccacgcg
     2761 ctgtcccttc ccgccgagcg cagcgccatc gaggtgctga tcgcgaacgg cgtggacgtg
     2821 cgggtcgact cgcgggatgc ctgggtgccg acgccggcgc tcagccacgc catcctcacc
     2881 tacaaccgcg gccgcgccgc tgacgattcc ggccgcgccg acggcatcgt cgtgaccccg
     2941 tcgcacaacc cgccccgcga cggcggcttc aagtacaacc cgccgcacgg cggacccgcc
     3001 gacacggacg ccaccgggtg gatcgcccag cgcgccaacg aactgatcga ggcgaagctg
     3061 gacggcgtca agcgcgagag cttcgccgac gtcgactggg acgccatcac cggatacgac
     3121 ttccgcgacg cgtacgtgcg ggacctgccg aacatcatcg acatcgacgc gatcaagcgt
     3181 gcgggcgtgc ggatcggcgc cgacccgctg ggcggcgcct cggtggagta ctggcagctc
     3241 atcaaggaca tgcacgggct cgacctgacg gtcgtcaacc ccgaggtcga cccgacctgg
     3301 cgcttcatga cgctggactg ggacgagaag atccggatgg atccgtcgtc gccgtcggcg
     3361 atggcctccc tggtcgccaa gaagggcgac ttcgacgtgc tcaccggcaa cgacgccgac
     3421 gccgaccggc acggcatcgt gaccccggat gccgggctga tgaaccccaa ccactacctg
     3481 gccgtcgcga tcgactacct gttctcgcac cgcgcctcgt ggccgcgtga cgccgcgatc
     3541 ggcaagaccc tggtgtcgtc gatgatcatc gaccgggtcg cggagtcgct gggccgccgc
     3601 ctgctggagg tgccggtcgg cttcaagtgg ttcgtccccg gtctgctgga cggatccgtc
     3661 gcgttcggcg gcgaggagtc cgccggtgcg tcgttcctgc gccacgacgg gagcgtgtgg
     3721 tcgaccgaca aggacggcat cctgctgtgc ctgctggctg ccgagatcat cgcggtgacc
     3781 ggcaagtccc cctctgagcg gtacgccgag ctggaggccg ccttcggggc atccgcctac
     3841 cagcgcgtgg acgcgccggc gacaccggag cagaaggcga cgctcggcaa gctggcaccg
     3901 gatgccgtga ccgccacaga cctcgccggt gaggagatca cggcgaagct ctcgcacgcc
     3961 cccggcaacg gtgcggcgat cggcggcctg aaggtgcaga ccgagcacgc ctggttcgcc
     4021 gcgcgcccgt cgggcacgga ggacgtctac aagctgtacg ccgagagcct gctcggggcg
     4081 gatcacctcg cccaggtgca ggaggaggcg cgcgcggtcg tcagcgcagc cctcggcggc
     4141 tgacgccctg cccgcggccg actgcccgcg agacttactt tgtgcggtga cgcttccgca
     4201 ggccggccaa gtgtcacccc gcaacgcaag tctcgcgggc gggtcaatgc ccaccttcgc
     4261 gagacttaac ttgtgcggtg aggcttgtcc aggctggccc tgtctcaccc tgcaacgtaa
     4321 gtctcgcggg tgtgtgaggg ccggtcagtg cgcgcgggcg agtgcagtac ccgcagcgcg
     4381 cagccactcg gcggcgtccg cctgcgccgc ctcaccggag ccggccaggt ccgtcaggg
//
LOCUS       DATMSX010000508         5380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_83266,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000508 DATMSX010000000
VERSION     DATMSX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83266"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VN041_16355"
     CDS             <1..930
                     /locus_tag="VN041_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30654.1"
                     /translation="DAAASAPAASGVPTPTASRSGRRFAARMQLPGMWFALPAAALLL
                     IFFAYPLAVSLLQSFFKTSGGVSTFVGFDQYARMLRDPLILKSLGNALLILVVQVPIM
                     IALAVGLAYLLNQAWLRFRSGFRLITFLPAITTLVAYSVVFRVIMTTDGGLLNQALAV
                     FGVAPIDWLNNELWARVAVIASITWRWTGYNMVIILAGLQAIPQELYEAARIDGAGRW
                     QIFWHVVVPQLRPVLIFTSVTSTIGALQLFDENFILTGGGPNNATLTPVLYLYKIGFQ
                     QFDFGYASAIAWMLVILTAIIAVVQFRIMREKP"
     gene            927..1820
                     /locus_tag="VN041_16360"
     CDS             927..1820
                     /locus_tag="VN041_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30655.1"
                     /translation="MSTQLITTGRAPRTGTPARSGEPLARKMILRGPVYLLLAGAGIL
                     ALVPFLWMVIASTHKTSDLFVTPLPLLPGGEFWQNLGRLQESVGFGQVMLNSVLVAVI
                     YTLFSTIVSVMCGYGLATYRFRGRGVLLGVILVTMMIPMQVLLVPLFQMMASVGWIDS
                     YQALIVPFLANAFGIFLMRQGFLDFPTSLIEAARIDGASELRTFYRIVLPVARPQIAA
                     LIIYTFISQWNSFIWPLLMLNTEEKYTIPVALNTMIGLSRVDYSGLMLGSLLATLPLL
                     VLFLVFQKQFVAGLLGGAVKG"
     gene            complement(1874..2530)
                     /locus_tag="VN041_16365"
     CDS             complement(1874..2530)
                     /locus_tag="VN041_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU30656.1"
                     /translation="MAIERKNLRSQVREELLARMRTGLVSPGESINEVQLAGELGVSR
                     TPLREALIALESEGQITSENGKGFRFVPLSAGEFEDLAPVMATLESLALELSPVEDLR
                     RVGAQLVEMAEAFTDEHVEHALVMTKDDEWHALMLSVCPNKRLIDVIESVRGSFHRYE
                     ALLVADDVKVDRVAAEHAAIAHALAAGDVPAAVEALKANWMHGMRRILENSSSAYFSA
                     "
     gene            2653..4755
                     /locus_tag="VN041_16370"
     CDS             2653..4755
                     /locus_tag="VN041_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWU30657.1"
                     /translation="MTQHRDDERIREIRIDALAYGGDYNPDQWSEETWHEDIRLMKEA
                     GVTMVSLPIFSWPQLEPEPGVYDWGWLDRIIDLLHEAGIRIDLATATATPPSWLLRAH
                     PEMGPSDSEGHRLEFGSRQTYCPSSPIWRENVARMTRAMADRYGEHPGLAMWHISNEY
                     GDHVSRCWCPESARHFRRWLRDRYGDLDGLNEAWGVNVWGQRYTSWEHIETPKKAPGP
                     INPTQLLDFERFSSDAMLELFQVEIDVLREVTPDIAVTTNFMSMFRDLDYWDFAERED
                     IVTDDAYPEPADPTSHVGAALNYGLMRSLKGGRPWLLLESSASATSWRDVNVPKAPGK
                     IRVESLQTVAHGSDGVMFFQWRQAKYGQEKFHSSMLGHRGERSRSYQETKALGNELRL
                     LEPVRGTRVRSRVALVVDWDSWWGSSAVESLPSQRLQWAQQARAWHRALHTLGHPVDA
                     VRATGPFDGYDVVVVPNLYIADEAQAVALRAFVENGGHLVVGPFSGVVDATEKVHDGG
                     APGPLRDLLGIEVDEQWPISDGLLERIAYGEDLLDVPSWAEWVDADADVEVRGCYASG
                     VLSGRAAVTRRAVGAGSAWYVSAMLAHDGMLAVFRDILRSSGLPARDVVNVDAEAVTR
                     TDGSTDYTFLLNHGSSPLAFELPASGTDLLSGTDLLSGADASGTVELGPYGAAVIASP
                     HAEAIPFLSLTDPTDPTE"
     gene            4764..>5380
                     /locus_tag="VN041_16375"
     CDS             4764..>5380
                     /locus_tag="VN041_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWU30658.1"
                     /translation="MTEAFVHPYIPNTAPAVKQAMLDAVGAESVEDFYADVPEDLRLR
                     RLLDVPAPLPAEQDLARHVKGILAANRSTEERLSFLGAGTYNHYVPAVVDEVIRRSEF
                     LTAYAGEPYEDHGRFQALFEYQSLMAELLAMDVVNVPTYDGYQATATGLAMAGRMTGR
                     RRVLALSDVLPEKLSKVVDYVRANLDVEAVATAGGVADLDALATAL"
BASE COUNT          793 a         1924 c         1755 g          908 t
ORIGIN      
        1 gacgccgcgg cgtccgcgcc cgcagcatcc ggagtcccga caccgaccgc ttcgcgatcc
       61 gggcgacgct tcgccgcccg gatgcagctc cccggcatgt ggttcgcgct gccggcggca
      121 gcgctgctgc tgatcttctt cgcgtacccg ctcgccgtct cgctgctgca gagcttcttc
      181 aagacctccg gcggcgtctc gacgttcgtc ggcttcgacc agtacgcgcg gatgctgcgc
      241 gacccgctca tcctgaagag cctgggcaac gccctgctga tcctggtggt gcaggtcccg
      301 atcatgatcg cgctcgcggt cgggctggct tatctgctca accaggcctg gctgcgcttc
      361 cgctccggct tccgcctgat caccttcctg ccggcgatca ccacactcgt ggcgtactcg
      421 gtggtgttcc gcgtgatcat gaccaccgac ggcggtctgc tcaaccaggc gctcgccgtg
      481 ttcggagtgg cgcccatcga ctggctcaac aacgagctct gggcgcgtgt ggccgtgatc
      541 gcctcgatca cctggcgctg gacgggctac aacatggtca tcatccttgc gggcctgcag
      601 gccatcccgc aggagctgta cgaggccgcc cggatcgacg gcgcaggccg atggcagatc
      661 ttctggcacg tggtcgtccc gcagctgcgg ccggtgctga tcttcacgag cgtgacgtcc
      721 accatcgggg cgctgcagct gttcgacgag aacttcatcc tgaccggtgg cggtcccaac
      781 aacgccacgc tcacccccgt gctgtacctg tacaagatcg ggttccagca gttcgacttc
      841 ggctacgcca gcgcgatcgc ctggatgctc gtcatcctca ccgcgatcat cgccgtcgtg
      901 cagttccgca tcatgaggga gaagccgtga gcacgcagct gatcaccacc ggccgggctc
      961 cgcgcaccgg cacacccgcg cgcagcggcg agcctctcgc gaggaagatg atcctgcgcg
     1021 gacccgtgta cctgctcctg gccggagcgg gcatcctcgc cctggtgcct ttcctgtgga
     1081 tggtcatcgc ctccacgcac aagacctccg acctgttcgt cacaccgctc ccgctgctgc
     1141 ccggcggcga gttctggcag aacctgggcc gtctgcagga gagcgtcggc ttcggccagg
     1201 tgatgctcaa cagcgtgctg gtcgccgtca tctacacgct gttcagcacg atcgtcagcg
     1261 ttatgtgcgg ctacggcctc gccacgtacc gcttccgcgg tcgcggcgtg ctgctcggcg
     1321 tcatcctcgt cacgatgatg atcccgatgc aggtgctgct ggtgccgctg ttccagatga
     1381 tggcgagcgt cggctggatc gactcgtacc aggcgctgat cgtgccgttc ctggcgaacg
     1441 ccttcggcat cttcctgatg cgccagggat tcctcgactt cccgaccagt ctcatcgagg
     1501 cggcccggat cgacggggca tccgagctgc gcaccttcta ccggatcgtg ctgccggtcg
     1561 cgcggcccca gatcgcggcg ctgatcatct acacgttcat cagtcagtgg aactcgttca
     1621 tctggccgct gctcatgctg aacaccgagg agaagtacac gatcccggtg gcgctgaaca
     1681 ccatgatcgg cctgagccgc gtggactact cgggtctgat gctgggctca ctgctggcca
     1741 ccctgccgct gctcgtgctg ttcctggtgt tccagaagca gttcgtcgcg ggtctgctgg
     1801 gcggcgcggt caagggctga gaaccgagca gggggcggat gccgttcggc atccgccccc
     1861 tgctccatcc gcctcaggcg gagaagtatg cgctggagga gttctccagg atgcggcgca
     1921 tgccgtgcat ccagttggcc ttgagcgcct cgacggccgc cggcacgtcg ccggcggcga
     1981 gggcgtgcgc gatcgcggcg tgctcggcgg ccacgcggtc gaccttgacg tcgtcggcga
     2041 ccagcaacgc ctcgtagcgg tggaacgaac cgcgcacgct ctcgatcacg tcgatcagcc
     2101 gcttgttggg gcacaccgac agcatcagcg cgtgccactc gtcgtccttg gtcatcacga
     2161 gcgcgtgctc gacgtgctcg tcggtgaacg cctcggccat ctcgaccagc tgcgcgccga
     2221 cgcggcgcag gtcctcgacg ggggagagct cgagcgcgag gctctcgaga gtcgccatca
     2281 cgggggcaag gtcctcgaac tcgccggcgc tgagcggcac gaagcggaag cccttgccgt
     2341 tctcgctggt gatctgcccc tcggactcga gggcgatcag cgcttcgcgc aggggggtgc
     2401 gactgactcc gagctccccg gccagctgca cctcgttgat gctctcgccc gggctgacca
     2461 ggccggtgcg cattcgcgcg aggagctcct cgcgtacctg tgagcggaga ttcttgcgct
     2521 cgatcgccat gttgtggccc cttcccctgc gtcagcttag agcttgaccc gatccatagt
     2581 tcggcatacg cttgctgtat acagaataca ttgaacagtg attctggcga agcatctcag
     2641 agaggagtcc tcgtgaccca gcaccgcgac gacgagcgca tccgggagat ccggatcgat
     2701 gctctcgcct atggcggcga ctacaacccc gaccagtggt cggaggagac gtggcacgag
     2761 gacatccgcc tcatgaagga ggccggggtc accatggtga gcctgccgat cttcagctgg
     2821 cctcagctcg agcccgagcc cggcgtgtac gactggggct ggctcgaccg catcatcgac
     2881 ctgctgcacg aggccggcat ccgcatcgac ctcgcgaccg cgaccgcgac cccgccgtcg
     2941 tggctgctgc gcgcccatcc tgagatggga ccctccgact ccgaggggca ccggctcgag
     3001 ttcggctcgc gccagaccta ctgcccgtcc tcgccgatct ggcgtgagaa cgtcgcgcgc
     3061 atgacccgcg ccatggccga tcgctacggc gagcatccgg gtcttgccat gtggcacatc
     3121 tccaacgaat acggcgacca cgtctcccgc tgctggtgcc ccgagtccgc ccgccacttc
     3181 cgccgctggc tgcgggaccg ctacggcgac ctcgacggcc tcaacgaggc ctggggcgtg
     3241 aacgtctggg gtcagcgcta cacgtcgtgg gagcacatcg agacgccgaa gaaggcaccg
     3301 ggcccgatca acccgaccca gctgctggac ttcgagaggt tctcgtcgga tgccatgctc
     3361 gagctcttcc aggtcgagat cgacgtgctc cgcgaggtca ccccggacat cgccgtgacg
     3421 accaacttca tgagcatgtt ccgcgacctg gactactggg acttcgccga gcgcgaggac
     3481 atcgtcaccg acgacgccta cccggagccc gccgacccca cctcgcacgt gggcgccgct
     3541 ctcaactacg ggctgatgcg ctcgctcaag ggcggccgcc cctggctgct gctggagtcg
     3601 tcggcgagcg ccaccagctg gcgcgacgtc aacgtgccga aggccccggg gaagatccgc
     3661 gtggagagcc tgcagaccgt cgcgcacggc tcggacggcg tgatgttctt ccagtggcgc
     3721 caggccaagt acggccagga gaagttccac tcctcgatgc tcggccaccg gggcgagcgc
     3781 tcgcgcagct accaggagac caaggcgctc ggcaacgagc tgcgcctgct ggagcccgta
     3841 cgcggcaccc gggtgcgctc gcgcgtcgct ctggtcgtgg actgggactc ctggtgggga
     3901 tcgagcgcgg tcgagtcgct gccgtcccag cgcctgcagt gggcccagca ggcccgcgcc
     3961 tggcatcgtg cgctccacac gctcgggcat ccggtcgacg cggtgcgcgc caccggcccg
     4021 ttcgacgggt acgacgtggt cgtcgtcccc aacctctaca tcgcggacga ggcgcaggcg
     4081 gtggcgctgc gcgccttcgt cgagaacggc ggccacctgg tggtcggccc gttctcgggt
     4141 gtggtggacg ccaccgagaa ggtgcatgac ggcggtgccc ccggcccgtt gcgtgatctg
     4201 ctcggcatcg aggtcgacga gcagtggccg atctcggatg gacttctcga gcgcatcgcc
     4261 tacggcgagg acctgctcga cgtgcccagc tgggcggagt gggtcgacgc ggacgccgac
     4321 gtcgaggtgc gcggatgcta cgcctcgggc gtgctctcag gtcgcgccgc cgtcacccgg
     4381 cgcgcggtcg gcgcgggctc ggcctggtac gtcagcgcga tgctcgcgca cgacgggatg
     4441 ctggcggtct tccgcgacat cctgcgcagt tcgggcctgc ccgcacggga cgtcgtgaac
     4501 gtcgacgccg aggcggtcac ccgcacggac ggcagcaccg actacacgtt cctgctcaac
     4561 cacggctcgt cgcccctcgc gttcgagctg ccggcatccg gcaccgatct gctcagcggt
     4621 accgatctgc tcagcggggc ggatgcctcg ggcaccgtcg agctcggccc ctacggcgcc
     4681 gcggtgatcg catctccgca cgccgaggcg atcccgttcc tctccctgac cgaccccacc
     4741 gaccccaccg aatgaaggat gtcgtgaccg aagcattcgt ccacccgtac attcccaaca
     4801 cggcgccggc cgtgaagcag gccatgctcg acgccgtcgg cgcggaatcc gtcgaggact
     4861 tctacgccga cgtccccgag gacctgcgcc tgcgccggct cctcgacgtg ccggcgccgc
     4921 tgccggccga gcaggacctc gcgcgccacg tgaagggcat cctcgccgcg aaccgcagca
     4981 ccgaggagcg gctcagcttc ctcggggcgg gcacctacaa ccactacgtg cccgccgtcg
     5041 tcgacgaggt catccgccgc agtgagttcc tcaccgccta cgcgggcgag ccctacgagg
     5101 accacggccg cttccaggcg ctgttcgagt accagtcgct catggccgag ctgctggcga
     5161 tggacgtcgt gaacgtcccc acctacgacg gctatcaggc cacggcgacg gggctcgcca
     5221 tggccggccg catgaccggc cgccgccgcg tgctcgcgct cagcgacgtg ctgccggaga
     5281 agctgtccaa ggtcgtcgac tacgtccgcg cgaacctcga cgtggaggcg gtcgccacgg
     5341 ccggcggcgt cgccgacctc gacgctctcg ccaccgcgct
//
LOCUS       DATMSX010000509        11273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_93363,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000509 DATMSX010000000
VERSION     DATMSX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93363"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VN041_16380"
     CDS             <1..764
                     /locus_tag="VN041_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30659.1"
                     /translation="PQFAPAPQPTIHPQSAAAQVITPPMGWYAFPPSGQPRWWNGVDW
                     TAYKLKNGRPRADAYAVEPPGMGWVLGGMFALIGLMNLLMGLLNPGMGALSIVWFAVS
                     IVWFIGAGRDHARRRVPPPTTAPSVDAELRPFPGEADAAGAGWYVVTGAVLRWWTGTR
                     WAHYVLDRGRVRPTHFAPRAFRASMITCAVIGGLGLLSLVGGIVLANTTEESDAGIAL
                     AFLGGAFIFIALILLISVYARRYALILPAKPPTQR"
     gene            complement(768..3464)
                     /locus_tag="VN041_16385"
     CDS             complement(768..3464)
                     /locus_tag="VN041_16385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWU30660.1"
                     /translation="MTTDTTTAPRRSIGSRIGRIAFVVLASLLVIATAAAFFLVWTIQ
                     RSFPETSGELSVKGLEKNVTVQRDALGIPTITASSTDDLFFAQGFTHAQDRFFEMDFR
                     RHVTSGRVAEMFGASQVPTDKFLRTLGWRKVAEQEVKALDDTTRGYYEAYAAGVNAYL
                     ASRSGADLSLEYAVLGVQNPDYRPEPWQPADSVAWLKAMAWDLRTNIGDETDRALLAA
                     QLSKAGESDADAKTLIDRVYPAYPFEQNPVIVPKISTVEPLAANADDEGGAAPASFST
                     KPGAGTDVSDAVTTVSDAISTVQWQQADSVIEAASMLLGPVGEGIGSNSWVVSGDLTA
                     SGKPLLANDPHLGAALPSVWYQMQLKCSTVSEKCPFDTAGFSFSGLPGIVIGHNEKIA
                     WGFTNLTTDVADLYIEKVQGDQYWRDGALQPIEVEKDVIKVAGGKDVPLTIRRTVHGP
                     IISGLTDDFTAIAGAPVVGTGKGSGEGVLPLADRPEIPQGDAAVSIRWTALDPGTTAK
                     AIFALDTAKDFTDFRRAASLFDVPAQNLIYADKKGNIGYQAPGRLPIRGAGDGWLPQP
                     GWDSAYDWTGYIPFEDLPVSYNPSDGYIVTANNAIVTDDYPYFLSRDWDYGYRAARIA
                     HLIERKAAAAPLKPQDLRDIQMDDEMWIGKQLAVAMADVKVEGEGPKAAVDLLRTWDA
                     QNRADSPAAAYANVLWSNLVRNVFIDREVPLPTGDQGRLFTVVGAMLDDPSDPLWTND
                     RLKADGMDAVLALSAKQAYSELSKLQGDDVTRWNWGSLHALTLTSSTLGSSGIAPIEM
                     LFNRGPYPVSGGSSLVNATGWKVGESYATTTVPSMRMVVDLADFDDSSWIHLTGASGH
                     AFNEHYTDQTEDWAAGVQQPWAFTKKAVAKATVDTLVLKPGS"
     gene            complement(3631..5814)
                     /locus_tag="VN041_16390"
     CDS             complement(3631..5814)
                     /locus_tag="VN041_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HWU30661.1"
                     /translation="MRNILQVLRFDLRRATSSVMAVIVLFGLVVIPSLFTWFNVIASW
                     NPFDNTKNLKVAVASTDEGYQSDLVPIRLNVGDQVLSTLRANHDLDWVFTSKDDAIDG
                     TRSGAYYAAIVLPPTFSTDMMTFYSNGAERTRIEYYTNEKKNALAPKITGQGAGEVAT
                     TINEAFTSTLSEAGLGIITSLSKYLDDADTQAALSRLQAHVGEVAAQLRAGASTADMF
                     TALIASSRPLVDSASGLASASGDALRSATGALGDGKQAAKSLKQTLASTTSALGDALS
                     GTAASYQAVADSVDDVFASMNAQATSSADSLRAVATRVQTQVDQHQRLRDDLVNTVRP
                     LLPESTLDAFDAAVSRIDAAIAREQALQDRLEEGATQIADGNADSQAMHAEISRLIAE
                     AKAAVQDARSAYTGSLQPKLDALAGTLASIGRDVDSIGGELSAAARSLTGSGSLDSAL
                     TDAESLTSGISQSLSETADRFDELDAALTKAIDTGDLSELSKVIGSDPKALATFIAEP
                     VELTRVPVYSVVSFGAAMTPLYTVLALWVGALLTAVSIRVDPPQGEGSPLPPLSPAET
                     YLGRFGIFAIVGFLQSSLVCMGSILFTQVQPQHPLLLLLAGWVSSLVFMLIIYTFVVT
                     FGNAGKALAVLLLVIQISGSGGAYPLQVLPQWFQNISPFLPATHAVNAMRSAIAGIYR
                     NDYWVSLGFLALFAVPALLLGLALRLPFIHANQKMLQALHSTKLM"
     gene            complement(5811..8684)
                     /locus_tag="VN041_16395"
     CDS             complement(5811..8684)
                     /locus_tag="VN041_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HWU30662.1"
                     /translation="MPNAWSVFRRDLGRLARVSKAWIIIIGVIVTPSLYAWFNIVAFW
                     DPYSNTQHVSVAIVNQDDGASSDLTGSIDVGGELVDQLKKNDQLGWEFLDEDEAMDAV
                     RSGRSYAAIIIPPDFSRDFLSITTGDFIRPELKYYVNEKANAIAPKITDVGASTLDNQ
                     INGTFVSVVSKAVTEQLKGVGADVEDRLGDARDSSVAALDDAVAKVSSARGRITELQS
                     GLTDAQSRLDSAAGTLGRVDKTLGKVQTAVAQAQSLAAEAQQELLTVTDKVTSAYVSG
                     ATLIADASSKMNASIATLSAGLQQANVAVGTAIDDLSAVVKANGAALDELQSVLDQTD
                     PGSEAAQKLRQAIDDLKQRNAHDQQVLDQLKALNGDVTNTIGSVKTAADKVNAATSQA
                     ATSAGAIRDALTKSVPELNRAMSALSASADGFSAALGAQRSQITQAQNLLAGLKTQLA
                     STTTALGALDGNLATAESGLSGVRTDVLALSAADIWNRLSTITGLDPEQIADFMASPV
                     EVHENAMFPTKAYGSAMAALFTNLTLWIGAFVLMVIFRLEVDTEGVEGVTVRQAYLGR
                     WMLFAAIGVCQAVLVSVGNLFIGVQTANAFAFVGTSVLIGLAYLSVIYALSVCFGYVG
                     KGLCILLVIFQIPGASGLYPIEMMPDFFRGLYPFLPFTYGIDALRETISGFYDGAWWR
                     AMGTLAIYVVLAFALGLFLRRRLGNFALLFNRRLSESQLLVSEDVQITGRRHTPQIIR
                     ALTDGDGYRAQVAERARRFTERYSTRLRLTLIVGAAVMAVIGVAAWLLPGAKATMLGL
                     WALWCLILIAFLVALEYIKQSIEQAAELGQMPDAELRQALIDETAGDGVLLAGAGASV
                     ASAGGTDAVRTAVLERTDVTTETDDDETDDDDTAVLDELFGPDAETDADVEAEADVET
                     EPDPADTEAVDTPEDAAAQHPEPDDAPLHDSEDHDAQEGGERA"
     gene            complement(8726..9358)
                     /locus_tag="VN041_16400"
     CDS             complement(8726..9358)
                     /locus_tag="VN041_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU30663.1"
                     /translation="MTTRAPRADALANRAGILDAARTALATDPHASVDVIARSAGLSR
                     RTLYGHFDDRDALIRELISTGAQRFNAIAESISDADARLALARLAARLWQEAAHVQFA
                     AALALDEAHVEHTADALAPLRRAVANLVRRGQDDGNFRTDLAAPTLARLIEEMARTVV
                     ARTDASSAGAANLAVRTVLSIAGLSWREADALLAAHPDIALASNAAEPAA"
     gene            complement(9598..11028)
                     /gene="argG"
                     /locus_tag="VN041_16405"
     CDS             complement(9598..11028)
                     /gene="argG"
                     /locus_tag="VN041_16405"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWU30664.1"
                     /translation="MSKVLQSLPVGEKVGIAFSGGLDTSVAVAWMREKGAIPFTYTGD
                     LGQYDESDIASIPGRALEYGAEASRLVDAKTALVEEGFVALSCGAFHIRSGGKTYFNT
                     TPLGRAVTGTLLVRAMREDGVDIWGDGSTYKGNDIERFYRYGLLANPRLRIYKPWLDA
                     DFVTELGGRQEMSEWLVERGFPYRDSAEKAYSTDANIWGATHEAKTLEHLNVSLETVD
                     PIMGVKYWDESVSIPTEDITVTFEGGRPVAINGVEYSDPVALVMEANTIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLFIAYERLVNGILNEDTLATYHEQGRRLGRLMYEG
                     RWLEPQSLMLRESIQRWVGLTISGSVTIRLRRGDDWTILDTVSPNLSYGPEKLSMERV
                     GDAAFGPVDRIGQLTMRNLDIADSRSRLEQYAGLGLVGGATGELVGKVTAGGANEIAA
                     SETVDEELAEAVDAASEGAAFDSGTD"
BASE COUNT         1659 a         3722 c         4054 g         1838 t
ORIGIN      
        1 ccccgcagtt cgcccctgcg ccgcagccca ccatccaccc gcagtccgcg gcggcgcagg
       61 tgatcacccc accgatgggc tggtacgcct tcccgccgtc cgggcagccg cgctggtgga
      121 acggcgtgga ctggaccgcc tacaagctca agaacggccg gccgagggcc gatgcatacg
      181 ccgtcgagcc cccgggaatg ggctgggtgc tcggtgggat gttcgcgctc atcggcctga
      241 tgaacctgct gatggggctg ctgaatcccg gcatgggggc gctgtccatc gtgtggttcg
      301 ccgtctcgat cgtgtggttc atcggtgcgg gccgcgatca cgcgcgtcgc cgggtcccgc
      361 cgcccacgac cgccccgtcg gtggacgccg agctgcgtcc gttccccggt gaggcggatg
      421 ccgccggagc cggctggtac gtcgtcaccg gcgcggtgct gcgctggtgg acgggaacgc
      481 gctgggcgca ctacgtactg gatcggggcc gcgtgcgccc gacgcacttc gctccgcggg
      541 cgttccgcgc ctcgatgatc acgtgcgcgg tcatcggcgg gctgggactg ctcagcctcg
      601 tcggaggcat cgtgctggcg aacacgaccg aggagtccga tgccggcatc gcgctcgcgt
      661 tcctgggcgg tgcgttcatc ttcatcgccc tgatcctgct gatcagcgtg tacgcccgcc
      721 gttacgcact gatcctgccg gcgaagccgc ccacgcagcg ctgacgctca ggaaccgggc
      781 ttcagcacca gggtgtcgac ggtcgccttc gcgacggcct tcttcgtgaa cgcccagggc
      841 tgctggacgc cggccgccca gtcctcggtc tggtccgtgt agtgctcgtt gaacgcatgg
      901 ccgctggcgc cggtgagatg gatccagctc gagtcgtcga agtcggcgag gtcgaccacc
      961 atgcgcatgg acggcacggt ggtggtcgcg tacgactcgc ccaccttcca gccggtggcg
     1021 ttcaccagtg acgagccgcc actgaccggg tacgggccgc ggttgaagag catctcgatc
     1081 ggggcgattc cggacgagcc cagcgtggaa ctggtcagcg tcagagcgtg cagcgagccc
     1141 cagttccagc gcgtcacgtc atcgccctgc agcttcgaca gctcgctgta cgcctgcttc
     1201 gccgagagcg cgagcacggc gtccatgccg tcggccttca gcctgtcgtt cgtccacagc
     1261 ggatcggagg ggtcgtccaa catggcaccc accaccgtga acagccgccc ctggtcgccg
     1321 gtgggcagcg gcacctcgcg gtcgatgaag acgttgcgca ccaggttcga ccacagcacg
     1381 ttggcgtagg ccgcggcggg ggagtcggcg cggttctggg catcccaggt ccgcagcagg
     1441 tcgaccgcgg ccttcgggcc ctcgccctcg accttcacat cggccatcgc cacggcgagc
     1501 tgcttgccga tccacatctc gtcgtccatc tggatgtcgc gcagatcctg cggcttgagg
     1561 ggagcggcgg ccgccttccg ctcgatcagg tgggcgatgc gcgccgcgcg gtagccgtag
     1621 tcccagtccc gtgacaggaa gtacgggtag tcgtcggtga cgatcgcgtt gttggccgtg
     1681 acgatgtagc cgtcactcgg gttgtacgag accggcaggt cctcgaaggg gatgtacccg
     1741 gtccagtcgt aggcgctgtc ccagccgggc tgcggcagcc agccgtcgcc cgcaccgcgg
     1801 atcggcagcc gcccgggggc ctgatagccg atgttcccct tcttgtccgc gtagatgagg
     1861 ttctgcgctg gcacgtcgaa cagggatgcc gcgcgacgga agtccgtgaa gtccttcgcg
     1921 gtgtcgagcg cgaagatcgc cttcgccgtg gttcccgggt cgagggcggt ccagcggatg
     1981 ctgacggccg cgtcgccctg cgggatctcg ggcctgtccg ccagcggcag cacgccctca
     2041 cccgagccct tgcccgttcc gaccacgggc gcgccggcga tcgccgtgaa gtcgtccgtg
     2101 agaccggaga tgatcgggcc gtgcacggtt cgtcggatgg tgagtggcac gtccttgccg
     2161 cccgcgacct tgatcacgtc cttctcgacc tcgatcggct gcagcgcacc gtcgcgccag
     2221 tactggtcgc cctgcacctt ctcgatgtag aggtcggcca cgtcggtggt gaggttggtg
     2281 aagccccagg cgatcttctc gttgtgtccg atcacgatgc cgggcaggcc cgagaaggag
     2341 aagccggctg tgtcgaacgg gcacttctcg ctgacggtgg agcacttcag ctgcatctgg
     2401 taccagaccg aggggagcgc ggcgcccaag tgcgggtcgt tcgcgagaag gggcttgccg
     2461 ctcgcggtga ggtcgccgga gacgacccac gagttggagc cgatgccctc gcccaccgga
     2521 cccagcagca tgctcgccgc ctcgatcacg ctgtcggcct gctgccactg cacggtgctg
     2581 atcgcgtcgg agacggtcgt cacggcgtcg gacacgtcgg tgccagcgcc cggcttcgtc
     2641 gagaaggatg ccggggccgc gccgccctca tcgtcggcat tcgccgccag tggttcgacg
     2701 gtggagatct tcggaacgat caccgggttc tgctcgaaag ggtacgccgg gtacacgcgg
     2761 tcgatgagcg tcttggcatc ggcatccgat tcgccggcct tgctcagctg cgccgcgagc
     2821 agcgcgcggt cggtctcgtc gccgatgttg gtgcgcaggt cccatgccat cgccttcagc
     2881 cacgccaccg agtcggcggg ctgccagggc tcgggcctgt agtccgggtt ctgcacgccg
     2941 agcaccgcgt actcgagcga cagatcggcg cccgagcgcg aggccaggta ggcgttcacc
     3001 ccggctgcgt acgcctcgta gtagccgcgg gtggtgtcgt cgagcgcctt gacctcctgc
     3061 tcggcgacct tgcgccagcc cagcgtgcgc aggaacttgt cggtggggac ctgagatgcc
     3121 ccgaacatct ccgcgacgcg gcccgaggtg acgtgccgcc ggaagtccat ctcgaagaac
     3181 cggtcctgcg cgtgggtgaa cccctgggcg aagaagaggt cgtcggtgga cgatgcggtg
     3241 atcgtgggga tgccaagggc atcgcgctgc acggtgacgt tcttctcgag ccccttcacg
     3301 ctgagctccc cggaggtctc ggggaaggag cgctggatgg tccagaccag gaagaaggcg
     3361 gcggcggtgg cgatgaccag cagggatgcc agcaccacga aggcgatgcg gccgatccgg
     3421 ctgccgatcg atcggcgggg tgcggtcgtg gtgtctgtcg tcatcggcgg gttccccagg
     3481 agtggatcgt gggaccccgt gtcacggagt ccgggtgcgc gtctcgggtg acgcgcaccc
     3541 cggcatccta tccactcgtt cggatgctgt cggttcgggg ttccgggtgc ggaatatctg
     3601 cactcggtcc gacaatctcc tggtcaggac tcacatgagc ttcgtggagt gcagcgcctg
     3661 cagcatcttc tggttggcgt ggatgaacgg cagacgcagc gccagcccga gcagcagcgc
     3721 cggaacggcg aacagggcca ggaagcccag cgagacccag tagtcgtttc ggtagatgcc
     3781 ggcgatcgcc gagcgcatcg cgttcaccgc gtgcgtggcg ggcaggaacg ggctgatgtt
     3841 ctggaaccac tgcggcagca cctgcagagg gtacgcgccg ccggagccgg agatctggat
     3901 gaccagcagc agcaccgcca gtgccttgcc cgcgttgccg aacgtcacca cgaacgtgta
     3961 gatgatcagc atgaacacca gggagctgac ccagccggcc aggagcagca ggagcggatg
     4021 ctgcggctgc acctgcgtga acaggatgct gcccatgcac accagcgagc tctgcaggaa
     4081 tcccacgatc gcgaagatcc cgaagcggcc gaggtaggtc tcggcggggg agagcggcgg
     4141 aagcggagag ccctcgccct gcggcggatc gacgcggatc gagaccgcgg tgagcagcgc
     4201 cccgacccac agggcgagca cggtgtacag cggcgtcatc gcggcgccga agctcacgac
     4261 cgagtacacc ggcacccgcg tcagctcgac gggctcggcg atgaacgtgg cgagggcctt
     4321 cgggtcggag ccgatcacct tgctgagctc gctcaggtcg cccgtgtcga tggccttggt
     4381 cagcgcggca tccagctcgt cgaagcggtc ggcggtctcg gagagcgact gcgagatccc
     4441 gctggtgagt gactcggcat ccgtcagcgc actgtccagc gaccccgatc cggtgagcga
     4501 cctcgcggcg gcggagagtt cgccgccgat cgagtcgaca tcgcggccga tcgaggcgag
     4561 cgtgccggcg agggcgtcga gcttcggctg caggctgccc gtgtatgcgc tgcgggcatc
     4621 ctgcaccgcc gccttcgcct cggcgatcag ccgggagatc tcggcgtgca tggcctggct
     4681 gtcggcattg ccgtcggcga tctgcgtggc gccctcctcc agccggtcct gcagggcctg
     4741 ctcgcgggcg atcgcggcgt cgatccggga cacggccgca tcgaacgcgt ccagcgtgct
     4801 ctccgggagc agcggacgca cggtgttcac caggtcgtcg cgcagcctct gatgctgatc
     4861 gacctgcgtc tgcacccggg tcgcgacggc ccggagcgag tccgccgagc tcgtcgcctg
     4921 cgcgttcatc gacgcgaaca cgtcatcgac gctgtcggcg accgcctgat agctggcggc
     4981 cgtgccggag agcgcgtcgc cgagcgcgga ggtggtcgag gcgagcgtct gcttcagcga
     5041 cttcgcggcc tgcttgccgt cgcccagcgc gccggtggcg ctgcgcaggg catcgccgga
     5101 agcggatgcc agccccgagg cgctgtcgac aagaggacgg ctcgacgcga tcagcgcggt
     5161 gaacatgtcg gcggtcgacg cacccgcgcg cagctgcgcg gccacctcgc cgacgtgggc
     5221 ctgcaggcgc gacagcgcgg cctgggtgtc ggcatcgtcg aggtacttgg acagcgacgt
     5281 gatgatgccg aggcccgcct cgctgagcgt ggacgtgaac gcctcgttga tggtcgtggc
     5341 gacctcgccg gcgccctgtc ccgtgatctt cggggccagc gcgttcttct tctcgttggt
     5401 gtagtactcg atgcgggtgc gctcggcacc gttcgagtag aaggtcatca tgtcggtgct
     5461 gaacgtcggc ggcagcacga tcgcggcgta gtacgcgccc gatcgggtgc cgtcgatcgc
     5521 gtcgtccttc gacgtgaaca cccagtccag atcgtggttc gcgcgcagag tggacagtac
     5581 ctggtcgccc acgttcaggc ggatcggcac gaggtcgctc tgatacccct cgtcggtgct
     5641 ggcgaccgcg accttgaggt tcttggtgtt gtcgaacggg ttccagctgg cgatcacgtt
     5701 gaaccacgtg aacagcgagg ggatcacgac caggccgaag agcacgatca cggccatgac
     5761 gctgctcgtg gcacggcgca gatcgaagcg gagcacctgc aggatgttcc tcatgcgcgc
     5821 tcccctcctt cctgggcgtc gtggtcttca gaatcgtgca gtggtgcgtc gtcgggttcc
     5881 ggatgctgtg cggcggcgtc ttcgggggtg tcgaccgcct cggtgtccgc aggatccggt
     5941 tccgtctcga cgtcggcctc cgcctcgaca tcggcgtccg tctcggcatc cgggccgaac
     6001 agctcatcca gcacggcggt gtcgtcgtcg tccgtctcgt cgtcgtccgt ctcggtggtg
     6061 acgtccgtcc gctcgagcac cgcggtgcgc acggcatccg tcccgcccgc cgatgcgacg
     6121 gatgccccgg cacccgcgag cagcacgccg tcgccggcgg tctcgtcgat cagcgcctgc
     6181 cgcagctcgg catccggcat ctgtcccagc tcggccgcct gctcgatgct ctgcttgatg
     6241 tactccaggg cgacgaggaa ggcgatcagg atcaggcacc acagcgccca caggccgagc
     6301 atggtcgcct tggcgcccgg cagcagccag gcggcgacgc cgatcacggc catcaccgcg
     6361 gcgccgacga tcagggtcag ccgaagccgg gtcgagtacc gctcggtgaa cctgcgcgcg
     6421 cgctccgcga cctgggcgcg gtagccgtcg ccgtcggtca gggcgcggat gatctgcggt
     6481 gtgtgacggc ggccggtgat ctgcacgtcc tcgctcacca gcagctggct ctctgaaagc
     6541 ctgcggttga acagcagagc gaagttgccg agccggcggc gcaggaacag cccgagcgcg
     6601 aacgccagca cgacgtagat cgcgagggtc cccatggccc gccaccaggc gccgtcgtag
     6661 aacccgctga tggtctcgcg cagggcatcg atgccgtagg tgaacggcag gaacgggtag
     6721 agcccgcgga agaagtccgg catcatctcg atcgggtaca ggccggatgc cccggggatc
     6781 tggaagatca ccagcaggat gcacaggccc ttgcccacgt agccgaagca caccgagagc
     6841 gcgtagatga cgctgaggta cgccaggccg atgagcacgc tggtgcccac gaacgcgaag
     6901 gcgttcgccg tctgcacgcc gatgaacagg ttgcccaccg agacgagcac ggcctggcac
     6961 acgccgatcg cggcgaacag catccagcgg ccgaggtagg cctgccgcac ggtgacgccc
     7021 tcgacgccct cggtgtcgac ctcgagacgg aagatcacca tcagcacgaa cgcgccgatc
     7081 cacagggtga ggttcgtgaa cagtgcggcc atcgccgagc cgtacgcctt ggtcgggaac
     7141 atcgcgttct cgtgcacctc gaccggtgag gccatgaaat cggcgatctg ctcggggtcg
     7201 agcccggtga tggtgctgag ccggttccag atgtcggcgg cgctgagcgc gagcacgtcg
     7261 gtgcgcacgc cgctcaggcc ggactccgcg gtggcgaggt tgccgtccag tgcgccgagc
     7321 gccgtggtgg tcgaggccag ctgggtcttc agccccgcca gcaggttctg cgcctgcgtg
     7381 atctgggagc gctgcgcacc gagagccgcc gagaatccgt ccgcgctggc cgacagcgcg
     7441 gacatcgcgc ggttgagctc ggggacgctc ttcgtcagcg catcgcggat ggctccggcc
     7501 gaggtcgccg cctgggaggt cgcggcatta accttgtcgg ccgcggtctt gaccgatccg
     7561 atcgtgttcg tgacgtcgcc gttcagcgcc ttcagctggt cgagcacctg ttggtcgtgg
     7621 gcattgcgct gcttcaggtc gtcgatggcc tggcggagct tctgtgcggc ctcggagccg
     7681 ggatcggtct gatccagcac ggactgcagt tcgtcgagcg cggcaccgtt cgccttgacg
     7741 accgccgaga ggtcgtcgat cgcggtgccg accgccacgt tcgcctgctg gagccccgcc
     7801 gagagcgtcg cgatcgaggc gttcatcttc gaggaggcgt cggcgatgag cgtggcaccc
     7861 gacacgtatg cggaggtcac cttgtcggtg acggtgagca gctcctgctg ggcctcggcg
     7921 gcgagggact gcgcctgcgc gaccgcggtc tgcaccttgc cgagcgtctt gtcgacccgg
     7981 cccagggtgc ccgccgcgct gtcgagccgg ctctgggcgt ccgtcaggcc gctctgcagc
     8041 tcggtgatgc gcccacgcgc ggaggagacc ttcgcgacgg cgtcgtcgag cgcggcgacc
     8101 gagctgtcgc gtgcgtcgcc gaggcgatcc tcgacatccg ctccgacgcc cttcagctgc
     8161 tcggtgacgg ccttcgagac cacggacaca aaggtgccgt tgatctggtt gtcgagggtg
     8221 gaggcgccga cgtcggtgat cttcggggcg atcgcgttcg ccttctcgtt gacgtagtac
     8281 ttcagttcgg ggcggatgaa atcgccggtg gtgatgctga ggaagtcgcg actgaagtcc
     8341 ggggggatga tgatcgcggc atagctgcgg ccgctgcgca cggcatccat cgcctcgtcc
     8401 tcgtcgagga actcccagcc gagctggtcg ttcttcttga gctgatcgac cagctcccca
     8461 cccacgtcga tgctgccggt gaggtcggag gaggcgccgt cgtcctggtt gacgatcgcg
     8521 acgctgacgt gctgcgtgtt cgagtagggg tcccagaacg cgacgatgtt gaaccatgcg
     8581 tacagcgagg gggtgacgat gacgccgatg atgatgatcc atgccttgct gacgcgtgcg
     8641 aggcggccga gatcgcggcg gaacacgctc cacgcgttgg gcatcgcacc tcccggcagg
     8701 ccagcggcat tccgagcata ggggctcacg cggcgggctc cgcagcattc gaggcgaggg
     8761 cgatgtcggg gtgggcggcg agcagggcat ccgcttcgcg ccaggagagc ccggcgatgc
     8821 tcagcaccgt gcgcacggcg aggttcgcgg cgcccgcgct cgacgcatcc gtgcgggcca
     8881 ccaccgtgcg cgccatctcc tcgatgaggc gggcgagggt gggagcggcg aggtcggtgc
     8941 ggaagttgcc gtcgtcctgc ccgcggcgca ccagattggc cacggcgcgg cggagaggtg
     9001 cgagcgcgtc ggcggtgtgc tcgacgtggg cttcgtccag ggcgagcgcg gccgcgaact
     9061 ggacatgtgc ggcctcctgc cacaggcgag cggccaggcg ggcgagggcg agtcgggcgt
     9121 cggcatcgga gatcgactcc gcgatggcgt tgaagcgctg ggccccggtc gagatcagct
     9181 cgcggatgag ggcgtcacga tcgtcgaagt ggccgtacag ggtgcggcgc gaaagcccgg
     9241 cgctgcgtgc gatcacgtcg acggaggcgt gcggatcggt ggccagcgcc gtgcgggcgg
     9301 catccaggat gccggcacgg ttcgccagcg cgtcggctcg gggcgcacgg gtggtcatgc
     9361 atccagggta cgtaacttgc acagtagtgt gcaagttgat tcgggggact cacagcatcc
     9421 ggcgccgtcc gccccgcggc atccggcacc ggccccgcca tcccgggtac agaaaggggg
     9481 ccgacacgcc agatgacgga cgttctcgct ggaaacgtcc gcaatctggc gtgtcggccc
     9541 ccaaagtgga aggagcagcc ggggccggga gggcaccggg ggcgtcggaa acgtgcgtca
     9601 gtcggtgccg gagtcgaagg ctgcgccctc ggatgccgca tccacggcct ctgcgagctc
     9661 ctcatcgacg gtctcggagg cggcgatctc gttcgctccg cccgcggtga ccttgccgac
     9721 gagctcgccg gtggcgccgc cgaccaggcc gaggcccgcg tactgctcca ggcgcgagcg
     9781 cgagtcggcg atgtcgaggt tgcgcatggt cagctggccg atgcggtcga ccgggccgaa
     9841 ggcggcgtcg cccacgcgct ccatcgagag cttctcgggg ccgtacgaca ggttcggcga
     9901 gacggtgtcg aggatggtcc agtcgtcgcc gcggcgcagg cggatggtga ccgagccgga
     9961 gatcgtcagg cccacccagc gctggatcga ctcgcgcagc atgagcgact gcggctccag
    10021 ccagcggccc tcgtacatca ggcgtcccag gcggcgtccc tgctcgtggt acgtggcgag
    10081 ggtgtcctcg ttcaggatgc cgttgaccag gcgctcgtag gcgatgaaca gcagtgccat
    10141 gcccggtgcc tcgtagatgc cgcgcgactt ggcctcgatg atgcggttct cgatctggtc
    10201 gctcatgccc agaccgtgac ggccgccgat cgtgttggcc tccatcacca gggccaccgg
    10261 gtcgctgtac tcgacgccgt tgatggcgac cgggcggccg ccctcgaacg tgaccgtgat
    10321 gtcctcggtg gggatcgaga ccgactcgtc ccagtacttc acgcccatga tcgggtcgac
    10381 ggtctcgagc gagacgttca ggtgctcgag cgtcttcgcc tcgtgcgtgg cgccccagat
    10441 gttggcgtcc gtggagtacg ccttctcggc cgagtcgcgg tacgggaagc cgcgctcgac
    10501 gagccactcg ctcatctcct gccggccgcc gagctcggtg acgaagtcgg cgtcgagcca
    10561 cggcttgtag atgcgcagac gcgggttggc gagcaggccg tagcggtaga accgctcgat
    10621 gtcgttgccc ttgtaggtgg agccgtcgcc ccagatgtcc accccgtcct cgcgcatggc
    10681 gcgcacgagc agcgtgccgg tgacggcacg gcccaggggg gtggtgttga agtacgtctt
    10741 gccgcccgag cggatgtgga acgcgccgca ggaaagggcg acgaagccct cctcgaccag
    10801 ggcggtcttc gcgtcgacca ggcgcgacgc ctcggcgccg tactccagcg cgcggccggg
    10861 gatcgaggcg atgtcgctct cgtcgtactg gcccaggtcg cccgtgtagg tgaaggggat
    10921 cgcacccttc tcgcgcatcc acgcgacggc gacggaggtg tcgagaccac cggagaatgc
    10981 gatgccgacc ttttcgccga cggggaggga ctggagcacc ttggacatgc gctcaagtct
    11041 atcggcgtgt cgcgtactgc cctcgcgtcg agtcgcggcg gggacggtgc agaacggggg
    11101 gccgacatgc cacatggcgg acgcatccag cgaaaacgtc cgtaatctgg cgtgtcggcc
    11161 cccgaagcgt aaaggcgggc gagcggcctc tgccgggggc ggggctcagc cccggctgag
    11221 gtccgcgaag cgctcgcgga gcggatgctg cgccccgagc cccgcgaggg aga
//
LOCUS       DATMSX010000510         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_16878,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000510 DATMSX010000000
VERSION     DATMSX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16878"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1079
                     /locus_tag="VN041_16410"
     CDS             165..1079
                     /locus_tag="VN041_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HWU30665.1"
                     /translation="MPTPKIVLFYAFAPLADPDAIRLWQRDLGEALRLRGRIIISKDG
                     INGTLGGDLPALKKWVRRFRDHAAFKDADIKWSEGSGIDAEGFSQDFPKLSVKVRDEI
                     VSFGAPGELRVDEHGVVGGGERLTPAQLHELMDERGDEVVFFDGRNALEAEIGRFRNA
                     IVPDTETTRDFVRLLDSGEYDDLKGKPVVTYCTGGIRCEVLSSLMVSRGFGEVYQLEG
                     GIVRYGEAYGDDGLWEGSLYVFDKRGSVDFSDHAAVIGECVGCGASTSRTANCPDSSC
                     RRQFVVCADCGAVPCAEHAAAAPLSASA"
     gene            complement(1063..3261)
                     /locus_tag="VN041_16415"
     CDS             complement(1063..3261)
                     /locus_tag="VN041_16415"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWU30666.1"
                     /translation="MSDRAREGASERRPVSPAGAGALTALAAFVSLWSYTGAVLPGVW
                     TFVLGVLIIVIVLTGVLMRLATARVRAGVRAVVVLIAQVIAAGAALTTMLAGETALLG
                     IIPTDQSVRLIGLRLQQSVDEIMNGVAPIAASMPMATLLGLAFAVVVILLDQLIAHRQ
                     VMLAVLFTSVVGVMPMLISFGAVNIAWFLMQAIVILLLLRFGASHDRRAPQGVSYSVA
                     TSAGVVAVVLALVLAPVLPVASALPGTGPMLTVNADLRLGEDLRRPEGIEALTLVTSA
                     ALPPYLRMATLSRFDGDVWRPDSGDRVPLGDGFGERDWAGPIKTVDTTVSIRVTGVSS
                     DRLPVPYAPQRVTDVGSGWSAMPGNRTVVSRTQDAAGADYTVQVATAAPTLEQIRASS
                     ATGPMAELPADLPPIISQLAHQVTANAGTDYDRLIALQDWFRTDFTYSLEAPVTEGFD
                     GTGADAVATFLEKRTGYCIHFAGAFALMASSLGMPVRIVVGYLPGTTTDEKRGDDVVY
                     SVSSDQLHSWPEAHFEGIGWVPFEPTATLGVPTDFASETAGGSNGDGPDAPQPSAGPS
                     SSATPTPTGGPAEERDPGGTGAVPLQRLDPTPVTLTVFGILIVLLLPMLVRELRRAAR
                     VGRARGGDAMAAWRELSDTLVDLGLAAPEAQTARVRADVLAMTRGVDPAALAPLVRAV
                     EHTSYAQSRPDAGNLANPLKIVIAQLAVSVDSRQRVLARLLPASLFTRRR"
BASE COUNT          502 a         1148 c         1225 g          445 t
ORIGIN      
        1 ggtcggcgtt cccggtgccg gcgcgggcct gcccgaagcc gacggcgtcc tcgcggccgt
       61 ggcaggactg ccatcggatc tgagcgcgga gctgatcccg gtcatcgccc cgaggtagcg
      121 tccgtcacgg ggcgtcctca gcccgcgcgg cctagaatct cctggtgccc acccccaaga
      181 tcgtgctgtt ctacgcgttc gcgccgctcg ccgacccgga tgccatccgg ctgtggcagc
      241 gcgacctcgg tgaggcgctg cgcctgcgcg gccgcatcat catctcgaag gacggcatca
      301 acggcacact cggcggcgat cttcccgcgc tgaagaagtg ggtgcgccgg ttccgcgacc
      361 acgcggcgtt caaggacgcg gacatcaagt ggagcgaggg ctccgggatc gacgcggagg
      421 ggttctccca agacttcccg aagctcagcg tgaaggtgcg ggacgagatc gtctcgttcg
      481 gcgctcccgg cgagctgcgg gtcgacgagc atggcgtggt cggcggcggc gagcggctga
      541 ctccggcgca gctgcacgag ctgatggacg agcgcggcga cgaggtggtc ttcttcgacg
      601 ggcgcaacgc gctcgaggcg gagatcggac ggttccggaa cgcgatcgtg ccggacaccg
      661 agaccacccg cgacttcgtg cgcctgctgg actcggggga gtatgacgac ctgaagggca
      721 agccggtcgt cacctactgc accggcggga tccgctgtga ggtgctctcc agcctgatgg
      781 tgtcgcgcgg cttcggagag gtgtaccagc tcgagggcgg catcgtccgc tacggcgagg
      841 cctatggcga cgacggcctg tgggaggggt cgctgtacgt gttcgacaag cgcggctcgg
      901 tcgacttctc cgaccacgcc gccgtcatcg gcgaatgcgt cggatgcggg gcatccacca
      961 gtcggacggc gaactgcccc gactcatcct gccgacggca gttcgtggtg tgcgcggact
     1021 gcggcgccgt gccctgcgcc gagcacgccg ccgcagcgcc gctcagcgcc tccgcgtgaa
     1081 cagcgacgcg ggcagcagcc gggccagcac gcgctgacga gagtccacgc tcacggccag
     1141 ctgcgcgatc acgatcttca gcgggttcgc caggttcccg gcgtccggcc tcgactgcgc
     1201 gtagctggtg tgctcgaccg cccgcaccag cggtgcgagg gctgcggggt ccaccccgcg
     1261 ggtcatggcg agcacgtcag cgcgcacgcg cgcggtctgc gcctcgggtg ctgccagccc
     1321 cagatccacg agtgtgtcgc tcagctcccg ccaggcggcc atcgcatccc cgccccgcgc
     1381 acgccccaca cgcgcggcgc gacgcagctc gcgcaccagc atcggcagca gcagcacgat
     1441 caggatgccg aacacggtca gcgtcaccgg ggtcggatcc agccgctgca gggggaccgc
     1501 cccggttccg cccggatcgc gctcctcggc cggaccgccg gtgggggtgg gcgtcgcgct
     1561 ggaggagggc ccggcgctcg gctgcggcgc atccggtccg tcgccgttgc tgccgcctgc
     1621 cgtctcggac gcgaagtcgg tcggcacgcc caacgtggcg gtcggctcga agggcaccca
     1681 gccgattccc tcgaagtgcg cctccggcca ggagtgcagc tggtcgctcg agacggagta
     1741 gacgacgtcg tcgccgcgct tctcgtccgt tgtcgtgccg ggcaggtaac cgaccacgat
     1801 ccgcaccggc atccccagcg acgaggccat cagcgcgaag gcgccggcga agtggatgca
     1861 gtagccggtg cgcttctcga ggaaggtcgc caccgcatcc gctcctgtgc cgtcgaagcc
     1921 ctcggtcacg ggagcctcga gcgagtaggt gaagtcggtg cggaaccagt cctgcagggc
     1981 gatcagccgg tcgtaatcgg tgcccgcgtt cgcggtgacc tgatgggcga gctgcgagat
     2041 gatcggcggg aggtcggcgg ggagctcggc catcgggccg gtcgcggagg atgcgcggat
     2101 ctgctccagc gtgggcgcgg cggtcgcgac ctgcacggtg tagtcggcgc cggcggcatc
     2161 ctgcgtgcgg ctgacgacgg tgcggttccc gggcatcgcc gaccagccgc tgccgacatc
     2221 ggtcacccgc tgcggagcgt acggcaccgg cagacggtcg ctggagacgc cggtgacccg
     2281 gatggagacg gtggtgtcga ccgtcttgat cgggcccgcc cagtcgcgct cgccgaatcc
     2341 gtcgccgagc ggcaccctgt cgccgctgtc cggccgccac acgtcgccgt cgaagcggga
     2401 cagcgtcgcc atgcgcaggt acggcggcag cgcggccgag gtcaccaggg tcagcgcctc
     2461 gatgccctcc gggcggcgca gatcctcgcc gagccgcagg tcggcgttga cggtcagcat
     2521 cggtccggtg ccgggaagag cggatgccac cggcaggaca ggcgcgagca cgagcgcgag
     2581 caccacggcg acgaccccgg ctgaggtggc cacggaatag gagacgccct gcggcgcgcg
     2641 ccggtcgtgg ctggcgccga accgcagcag cagcaggatg acgatggcct gcatcaggaa
     2701 ccaggcgatg ttcacggcgc cgaaggagat cagcatcggc atgacgccga ccaccgaggt
     2761 gaacagcacg gcgagcatca cctgccggtg cgcgatcagc tggtcgagca ggatcacgac
     2821 caccgcgaag gccaggccca gcagggtcgc catcggcatg gatgccgcga tcggggcgac
     2881 gccgttcatg atctcgtcga cgctctgctg cagccgcagt ccgatcaggc gcacggactg
     2941 atcggtgggg atgatgccca gcagcgcggt ctcgccggcc agcatggtgg tgagtgccgc
     3001 gcccgccgcg atcacctgcg cgatcagcac cacgacggcg cggacgccgg cgcgcacgcg
     3061 cgcggtcgcg aggcgcatga ggacgccggt gagcacgatc acgatgatca gcactccgag
     3121 cacgaacgtc cacacgccgg gcagcacggc gcccgtgtac gaccacagtg agacgaaggc
     3181 ggccagcgcc gtcagcgcgc cggcgccggc cgggctgacc ggtcgacgct cggatgcgcc
     3241 ttcgcgggca cggtcagaca tgcgacaccg tcgagaaggg gaggacggat gcccaggtgt
     3301 cggcgaggtc cacggcggag
//
LOCUS       DATMSX010000511         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_46204,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000511 DATMSX010000000
VERSION     DATMSX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46204"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN041_16420"
     CDS             complement(<1..455)
                     /locus_tag="VN041_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWU30667.1"
                     /translation="MTLAARLSAIDDRIAEAARRAGRDPGEITRIVVTKFHPAQLVRD
                     LRALGVTEVGENRQQELTAKRDDLVDLDDVRWHFIGQAQTNKAAAIRRASDVVHSVDR
                     TRLADALHRAADDDGVLDVLVQVNLTEDAGRGGSTLADAEALAEHVLGL"
     gene            complement(452..1597)
                     /gene="ftsZ"
                     /locus_tag="VN041_16425"
     CDS             complement(452..1597)
                     /gene="ftsZ"
                     /locus_tag="VN041_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWU30668.1"
                     /translation="MSQNQNYLAVIKVVGVGGGGVNAVNRMIDLGLRGVEFIAVNTDA
                     QALLMSDADVKLDVGRELTRGLGAGADPEVGRRAAEDHAEEIEQALTGADMVFVTAGE
                     GGGTGTGGAPVVARIAKSIGALTIGVVTKPFSFEGRRRQSQAEAGVAKLKEEVDTLIV
                     VPNDRLLEISDRGISMIEAFATADQVLLAGVQGITDLITTPGLINLDFADVKSVMQGA
                     GSALMGIGSARGADRAIKAAELAVESPLLEASIEGAHGVLLSIQGGSNLGIFEIHDAA
                     DLVKEAAHPEANIIFGTVIDDTLGDEVRVTVIAAGFDGGEPAPRLEPFDVKRPEAAPL
                     PEVRVDEKAVEDVVVEPVKPTVSVSAPTSIEPAFPDDGDLDIPEFLK"
     gene            complement(1748..>2477)
                     /locus_tag="VN041_16430"
     CDS             complement(1748..>2477)
                     /locus_tag="VN041_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWU30669.1"
                     /translation="AARARRKALRAEIRRFTQRSRRRRIVWWTAIGAVVALVLGSVLA
                     AYSPLFAVEKITVAGVKSLDPAAVQKALEGEIGSPLALIDDSAVKSALTEFPMIESYS
                     LEARPPHELLVRIVERTPVGVIRTDGGYTLVDAAGVVLATTADKPEGQPVLSITGGTD
                     SAAFRSAGLVIRSLPADLRGQVTAVSASTLDDVTLTLADGKTVLWGSEDDSVTKALVL
                     VALIKSSPKGQSFDVSAPSVPVVR"
BASE COUNT          361 a          869 c          860 g          387 t
ORIGIN      
        1 tcgaggccga gcacgtgctc ggccagcgcc tcggcatccg ccagcgtgct tccgccgcgc
       61 ccggcgtcct ccgtgaggtt cacctgcacg agcacgtcga gcacgccgtc gtcgtcggcg
      121 gcgcgatgca gcgcatccgc cagtcgcgtc cgatcgaccg agtggaccac gtcggatgcc
      181 cgccggatgg cggcggcctt gttcgtctgc gcctggccga tgaagtgcca gcgcacgtcg
      241 tccaggtcca cgaggtcgtc gcgcttggcg gtgagctcct gctggcggtt ctcgcccacc
      301 tcggtcacgc cgagggcgcg cagatcgcgg accagttggg cggggtggaa cttggtgacc
      361 acgatgcggg tgatctcccc cgggtcgcga ccggcccgcc gcgcggcctc ggcgatgcgg
      421 tcatcgatcg ccgagagccg cgctgcgaga gtcacttcag gaactcgggg atgtcaaggt
      481 cgccgtcgtc cgggaacgcc ggctcgatgc tggtgggagc cgagacggac accgtcggct
      541 tcaccggctc gaccacgacg tcctcgacgg ccttctcgtc cacgcgcacc tcgggcagcg
      601 gagcggcctc gggacgcttg acgtcgaacg gctccaggcg cggcgccggc tcgccgccgt
      661 cgaacccggc cgcgatgacg gtcacgcgca cctcgtcgcc gagggtgtcg tcgatgacgg
      721 ttccgaagat gatgttcgcc tcggggtgcg cggcctcctt gacgaggtcg gccgcgtcgt
      781 ggatctcgaa gatacccagg ttcgacccac cctggatcga cagcagcacg ccgtgggcgc
      841 cctcgatgga cgcctccagc agcggggact cgacggcgag ctcggcggcc ttgatcgcac
      901 gatccgcgcc gcgcgcggat ccgatgccca tcagggcgga gccggcgccc tgcatgaccg
      961 acttgacgtc ggcgaagtcg aggttgatca gaccgggggt cgtgatgagg tcggtgatgc
     1021 cctgcacacc ggcgaggagc acctggtcgg ccgtggcgaa cgcctcgatc atggagatgc
     1081 cgcggtcgct gatctcgagg aggcggtcgt tcggcaccac gatgagggtg tcgacctctt
     1141 ccttgagctt ggcgacgccg gcctcggcct ggctctggcg gcgacgtccc tcgaacgaga
     1201 acggcttggt gacgacgccg atggtcagcg caccgatcga cttggcgatc cgcgcgacga
     1261 cgggcgctcc accggttccc gtgccaccgc cctctcccgc ggtgacgaag accatgtcgg
     1321 cgccggtaag cgcctgctcg atctcctcag cgtggtcctc ggcggcgcga cggcccacct
     1381 cggggtccgc accggcgccc agcccgcgcg tgagctcccg gcccacgtcg agcttcacgt
     1441 cggcgtcgct catgagcagc gcctgcgcgt cggtgttcac ggcgatgaac tcgaccccgc
     1501 ggagcccgag atcgatcatg cggttgacgg cgttgacgcc gccaccgccg acgccgacga
     1561 ccttgatcac ggcgaggtag ttctggttct ggctcatggc cggcctccgt tattcgagcc
     1621 tgacgaggga gaaaccttaa acctcaagaa gagggttaaa ctatttcccg gtattgcgtt
     1681 ctcgtcatcg acgctaagcg caaccgacgc cgctgcccgc accgacacgg cgtgtcgtcg
     1741 cgatccctca gcggacgacc ggaacagagg gtgccgacac gtcgaagctc tgccccttgg
     1801 gagagctctt gatgagggcg acgagtacca gcgccttcgt gacggagtcg tcctcgctcc
     1861 cccacagcac ggtcttgccg tcggcgaggg tcagtgtgac gtcgtcgagg gtggatgcgg
     1921 ataccgcggt gacctgcccc cgcagatcgg ccggaaggga gcggatgacg agcccggcgc
     1981 tgcggaacgc ggcggagtcc gtgccgccgg tgatactcag gaccggctgt ccctccggct
     2041 tgtccgcggt ggtggccagc acgacccccg ccgcgtcgac gagcgtgtag ccgccgtcgg
     2101 tgcggatcac tcccaccggc gtgcgctcca cgatgcggac cagcagctcg tgcggcgggc
     2161 gggcctccag ggagtacgac tcgatcatcg ggaactccgt gagcgcggac ttcacggcgc
     2221 tgtcgtcgat cagcgcgagc ggagacccga tctcgccttc gagcgccttc tgcaccgctg
     2281 ccggatcgag ggacttcacc ccggccaccg tgatcttctc cacggcgaac agcgggctgt
     2341 atgcggcgag gacgctgccg agcacgagcg cgaccacggc accgatcgcg gtccaccaga
     2401 cgatcctcct gcgacgcgac cgctgggtga agcggcggat ctccgcgcgc agcgccttcc
     2461 gccgcgcgcg ggccgcg
//
LOCUS       DATMSX010000512         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_93397,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000512 DATMSX010000000
VERSION     DATMSX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93397"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..242)
                     /locus_tag="VN041_16435"
     CDS             complement(24..242)
                     /locus_tag="VN041_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30670.1"
                     /translation="MPAPHGFEHLVRGDEIVILHHGIRAAVLRGAKAADFLARIDTED
                     PQLLMARLTGNYRRGNERAARRHPRNQS"
     gene            complement(247..1812)
                     /locus_tag="VN041_16440"
     CDS             complement(247..1812)
                     /locus_tag="VN041_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWU30671.1"
                     /translation="MTSPASLLPPLDDRATLWERLRDRAMGNPSTARMLGWLTPLLIT
                     LLAGVLRLVNLAHPHQIMFDETYYVKDAWSLWNLGYEAKWGDNADAHLLTGDDSAMQT
                     AGSFVVHPPLGKWIIALGIAAFGPGSSLGWRLTTAILGTLTVLLVYLIALLLTRSRTV
                     AAIAGGLLAVDGLGIVMSRIALLDGILAFFVMLGVLFVLLDRRRTMPLLAMIGSGRDP
                     NDPPLMNGPVLWARPWLIAAGVVFGASAAVKWSGVYALAVFGLYVVVTDALARRRAGV
                     ALWPTDAVFRQGPASFLLSVGPALVTYLVAWTGWLVTSGGYGRQADADPLVALWKYHE
                     AILKFHVGLHSPHPYASPAWQWPLLLRPTAIWVGDKPTGCGTDHCIAVISSVPNPLIW
                     YASIAAALYLLYRFVRGLIDRRPAGPALTLALVGLVATYVPWLLVGNRTIFQFYTIAM
                     VPFLVIGLAVALRELAGRKDAPLHRRQAGQRTVVVYLCVVVLVSVFYYPVWTGMSVPY
                     EFWLIHNWLPGWI"
     gene            1861..2676
                     /gene="rsmI"
                     /locus_tag="VN041_16445"
     CDS             1861..2676
                     /gene="rsmI"
                     /locus_tag="VN041_16445"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWU30672.1"
                     /translation="MIILAATPIGNLGDASRRLIEVLENAEIVAAEDTRTTQRLLQAL
                     KIENRPRLIALHDHNEKHKAAELAALAEEQDVVVVSDAGMPAVSDPGYGLVAAAVERG
                     VTVTAIPGPSAVLMALAISGLPTDRFTFEGFLPRKPGERRSTLSALASEPRTMVFFES
                     PSRLASSLADMGAAFGADRRIAVCRELTKLYEEVRRGTASELAEWAGQGVKGEIVVVV
                     SGATPVAVSAEAAVAQVQALVASGVRLKDACAEVAAATGLSSRDLYQAALAAR"
     gene            2673..3281
                     /locus_tag="VN041_16450"
     CDS             2673..3281
                     /locus_tag="VN041_16450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30673.1"
                     /translation="MSDVIGGAYDARAAEYIALIGEVDQLAERDREEIGGWADAVPGR
                     ILDAGCGPGLWTRFLHDRGHEALGIDLSDQFIAHARARNPHLEFLHGSFERVPLPDAS
                     LGGILAWYSLIHTPPEELPAILAAFARLLAPGGGILIGFFDGVPREPFPHAVTTAYFW
                     SAESLAALLDAAGFTVTAVEHRGRMPGEVSARPHGAVTAIRS"
     gene            3580..>5052
                     /gene="metG"
                     /locus_tag="VN041_16455"
     CDS             3580..>5052
                     /gene="metG"
                     /locus_tag="VN041_16455"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWU30674.1"
                     /translation="MPAGESFYITTPIYYPSDVPHIGHGYTSVAVDTLARWHRQAGDD
                     TWMLTGTDEHGQKMLRAAAANNVTPQEWVDKLVGESWFPLLKTLDVANDDFIRTTQER
                     HEKNVQVFFQKLYDAGYIYAGEYEALYCVGCEEFKPESEIVDGTGPFEGLKVCAIHSK
                     PLELLQEKNYFFKLSEFQDRLLELYKTEPDFVRPDSARNEVVSFVSQGLKDLSISRST
                     FDWGIPLPWDESHVIYVWVDALLNYVTAVGYGSDESTFDRRWPAYHVVGKDILRFHAV
                     IWPALLMAAGLEVPKGVFAHGWLLVGGEKMSKSKLTGIAPTEITDVFGSDAYRFYFLS
                     AIAFGQDGSFSWEDLSARYQAELANGFGNLASRTIAMIEKYFDGVVPEASDYTDADLA
                     IQKTVADAASKADAAIGHFRIDEAIHSIWTIVDALNGYITENEPWALARDEAQRARLG
                     TVLYTCAEGLRALAVLLSPVMPQSTGTLWEALGVEDSLGRS"
BASE COUNT          817 a         1808 c         1692 g          735 t
ORIGIN      
        1 ccgcgcactg agccctgctc ggctcaggac tggttgcgcg gatgccggcg ggcggcgcgc
       61 tcgttgccgc gccggtagtt cccggtcagg cgcgccatca gcagctgcgg atcctcggtg
      121 tcgatccgcg ccaggaagtc ggccgccttc gcgcccctca ggacggccgc gcggatgccg
      181 tgatgcagga tcacgatctc gtcgccgcgg accagatgct cgaagccatg aggtgcgggc
      241 atgggctcag atccagcccg gtagccagtt gtgaatgagc cagaactcat agggcacgct
      301 catgcccgtc cacaccgggt agtagaacac cgacaccagc acgacgacgc acagatacac
      361 gacgacggtg cgctggccgg cctgccggcg gtgcagcggg gcatccttcc ggcctgcgag
      421 ctcccgcagc gcgaccgcga gcccgatcac caggaacggc accatcgcga tcgtgtagaa
      481 ctggaagatc gtgcggttgc cgaccagcag ccacggcaca tacgtcgcga ccagtcccac
      541 cagcgcgagg gtcagagcgg ggccggcggg cctccggtcg atgagcccgc gcacgaaccg
      601 gtacagcagg tacagcgcgg cggcgatgct cgcgtaccag atcagcgggt tcggcaccga
      661 ggagatcacc gcgatgcagt ggtcggtacc gcagcccgtc ggcttgtcgc cgacccagat
      721 cgccgtcggc cgcagcagca acggccactg ccaggccggg ctcgcgtacg ggtgcgggct
      781 gtgcagcccg acgtggaact tgaggatcgc ctcgtggtac ttccacaggg cgaccagcgg
      841 atcggcatcc gcctgccggc cgtacccgcc cgaggtgacc agccatcccg tccaggccac
      901 caggtaggtc accagtgcgg gtcccaccga gaggaggaag gatgccgggc cctgccggaa
      961 cacggcatcc gtcggccaca gcgcgacgcc cgcccgtcgc cgcgcgagcg catcggtgac
     1021 gaccacgtac aggccgaaga cagccagcgc gtacacgccc gaccacttga ccgcggcgga
     1081 tgctccgaag acgacgccgg ctgcgatcag ccacggtcgc gcccacagga ccggcccgtt
     1141 catcagcggc ggatcgttgg ggtcgcggcc tgagccgatc atcgccagca gcggcatcgt
     1201 gcgccgcctg tccagcagca cgaagagcac gcccagcatc acgaagaagg cgaggatgcc
     1261 gtccagcagc gcgatgcggc tcatcacgat cccgagcccg tcgaccgcga gcagcccgcc
     1321 ggcgatcgcg gcgaccgtgc gggaccgggt cagcagcagt gcgatcaggt acaccagcag
     1381 cacggtcagc gtgcccagga tcgcggtcgt cagccgccag cccagactgg aaccagggcc
     1441 gaacgcggcg attcccagcg cgatgatcca cttgcccagc ggcggatgca ccacgaacga
     1501 acccgcggtc tgcatcgcgg agtcgtcgcc ggtcagcagg tgcgcatcgg cgttgtcacc
     1561 ccacttcgcc tcgtagccca ggttccacag cgaccatgcg tccttgacgt agtacgtctc
     1621 gtcgaacatg atctggtgcg gatgggcgag gttcaccagc cgcagcacgc cggcgagcag
     1681 cgtgatcagc aggggcgtga gccagcccag catccgcgcc gtcgaagggt tccccatcgc
     1741 gcggtcgcgc agccgctccc agagcgtcgc acgatcgtcc agcggcggca gcagggaggc
     1801 gggcgatgtc acctgatcag cctaggcaat cccgtcggtc cgcccgcata agctgatcgg
     1861 gtgatcatcc tggcggcgac cccgatcggc aacctcggcg atgcgtcccg gcgcctgatc
     1921 gaggttctcg agaacgccga gatcgtcgcc gccgaggaca cccgcaccac gcagcggctg
     1981 ctgcaggcgc tgaagatcga gaaccggccg cggctgatcg ccctgcacga ccacaacgag
     2041 aagcacaagg ccgctgagct cgccgcgctt gccgaggagc aggacgtcgt cgtcgtcagc
     2101 gatgccggga tgcccgccgt gagcgacccc ggctacgggc tggtggccgc cgccgtggag
     2161 cggggcgtga cggtgaccgc catccccggg ccgagcgcgg tgctgatggc actggcgatc
     2221 tccggactcc ccaccgaccg cttcaccttc gagggcttcc tgccgcgcaa gccgggggag
     2281 cggcggtcga ccctttcggc gctcgcctcc gaaccccgca ccatggtgtt cttcgagtcg
     2341 ccgtcgcgcc tcgcctcctc gctcgcggac atgggtgccg ccttcggcgc cgaccggcgc
     2401 atcgcggtgt gccgcgagct caccaagctc tacgaggagg tgcgccgggg cacggcatcc
     2461 gagctcgcgg agtgggccgg gcagggggtg aagggtgaga tcgtcgtggt cgtgtcggga
     2521 gcgacgcccg tcgccgtctc cgccgaggcc gccgtcgccc aggtgcaggc actggtggcg
     2581 tccggcgtcc ggctcaagga cgcctgcgcc gaggtggccg ccgcgaccgg cctgagctcc
     2641 cgcgacctct accaggccgc cctcgccgcc cgatgagcga cgtcatcggg ggtgcgtacg
     2701 acgcgagggc tgcggagtac atcgcgctca tcggcgaggt ggatcagctc gcagagcggg
     2761 atcgcgagga gatcggcgga tgggcggatg ccgttcccgg gcgcatcctc gatgccggtt
     2821 gcggaccggg cctgtggacg cggttcctgc acgatcgcgg gcacgaggca ctcggcatcg
     2881 acctctccga tcagttcatc gcgcacgcgc gggcgcggaa tccgcacctc gagttcctgc
     2941 acggatcgtt cgagcgggtt ccgctgccgg atgcctcgct cggcgggatc ctcgcctggt
     3001 actcgctgat ccacacgccg cccgaggagc tccccgcgat cctcgccgcg ttcgcccgcc
     3061 tgctcgcccc gggcggcggc atcctgatcg gcttcttcga cggcgtcccg cgcgagccct
     3121 tcccgcacgc cgtcacgacc gcgtacttct ggagcgccga gtccctggct gcgctcctcg
     3181 acgccgcggg tttcacggtc acggccgtcg agcatcgagg tcggatgccg ggtgaggtca
     3241 gcgcgcgccc gcacggcgca gtgacagcaa tacgatcctg atcatcggcg ccgtcggatc
     3301 cccggcatcc atgcccacag catcccaccg ccgtgctcct ccagcggaga tgtgcgccaa
     3361 acgcggccta atcgtcaccg tccgtccgcg cgaagcgcac ttctccgcgg gagctgccca
     3421 ctttctcacc ccatctccgc acgccgaggg cagacccgca accgcgtcca cccgctctgc
     3481 gagcgtaggg aacctccgga tgcccgcgtc cggcccgcgg catccgcaca gcaggtcaca
     3541 cgacaatcgc cctcccgctt cccgcctaga atcgatccca tgcctgcagg cgagtctttc
     3601 tacatcacca cgccgatcta ctaccccagc gacgtgccgc acatcggcca cggctacacg
     3661 tccgtcgccg tggacactct tgcgcgctgg caccgccagg cgggggacga cacctggatg
     3721 ctgaccggca ccgacgagca cgggcagaag atgctccgtg ccgccgcggc gaacaacgtc
     3781 accccgcagg agtgggtcga caagctggtc ggcgagagct ggttcccgct gctgaagacc
     3841 ctcgacgtcg ccaacgacga cttcatccgc accactcagg agcggcacga gaagaacgtg
     3901 caggtgttct tccagaagct gtacgacgcg ggctacatct acgcgggcga gtacgaggcg
     3961 ctgtactgcg tgggctgtga ggagttcaag ccggagtccg agatcgtcga cggcaccggt
     4021 cccttcgagg ggctgaaggt ctgcgcgatc cactccaagc cgctcgagct gctgcaggag
     4081 aagaactact tcttcaagct cagcgagttc caggaccgcc tgctcgagct ctacaagacc
     4141 gagcccgact tcgtgcgccc cgactcggcg cgcaacgagg tcgtctcgtt cgtcagccag
     4201 gggctgaagg acctgtcgat ctcgcgctcg acgttcgact ggggcatccc gctgccctgg
     4261 gacgagtcgc acgtcatcta cgtgtgggtc gacgcgctgc tcaactacgt caccgccgtc
     4321 ggctacggtt ccgacgagtc gaccttcgac cgccgctggc cggcgtatca cgtggtgggc
     4381 aaggacatcc tgcgcttcca cgccgtgatc tggcccgcgc tgctgatggc ggcagggctg
     4441 gaggtgccca agggcgtctt cgcacacggc tggctgctgg tcggcggcga gaagatgtcg
     4501 aagtccaagc tcaccggcat cgcgcccacc gagatcaccg acgtgttcgg ctcggacgcg
     4561 taccgcttct acttcctctc ggcgatcgcg ttcggccagg acggctcgtt ctcctgggag
     4621 gacctgtcgg cccgctacca ggccgagctc gcgaacggct tcgggaacct cgcgtcgcgc
     4681 acgatcgcga tgatcgagaa gtacttcgat ggcgtcgtcc ccgaggcatc cgactacacc
     4741 gacgccgacc tcgcgatcca gaagacggtc gcggatgccg cgtcgaaggc ggatgccgcg
     4801 atcggccact tccgcatcga cgaggcgatc cactcgatct ggacgatcgt ggacgcgctg
     4861 aacggctaca tcacggagaa cgagccgtgg gcgctcgcgc gcgacgaggc tcagcgtgcg
     4921 cgcctcggca ccgtgctgta cacctgcgcc gagggcctgc gggcgctcgc cgtgctgctc
     4981 tcgccggtca tgccgcagtc gaccggcacg ctgtgggagg cgctgggcgt cgaggactcg
     5041 ctgggtagat cg
//
LOCUS       DATMSX010000513         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_74365,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000513 DATMSX010000000
VERSION     DATMSX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74365"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN041_16460"
     CDS             <1..495
                     /locus_tag="VN041_16460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30675.1"
                     /translation="TRLHPRGVAVVRTTPGGPLPFADGGFDLVTSRHPVSPDWQEITR
                     VLAPGGTYLAQHVGPASGFELIEYFLGPLPSERQGRDPQVEADAARAAGLEIVDLRTA
                     RLRIEIRDIGAVVYLLRKLVWWVPGFTVEKYRDRLRDLHEQIQRDGVFEDHSSRHLIE
                     ARRL"
     gene            complement(502..1329)
                     /locus_tag="VN041_16465"
     CDS             complement(502..1329)
                     /locus_tag="VN041_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWU30676.1"
                     /translation="MALQPYTSLPLDQGAVSYAYGPDSFPRPGVEPGRIDRFTIDDST
                     AYPGTTRSVWVHAPAGHDPLRPASVMLFNDGWWYLDPEGDVRAGVVLDNLAAQGAIPS
                     VVGVFVDPGIRTASDEPKNRNVEYDADDDRYAGFLIDEVLPRVAALHTLEPDGHGICG
                     GSSGGDAAFTAGWQRPEVFRRVIAFNPSFAQMPQGNPYPRLLSGGERRDLRIVLHAAH
                     RDIGWNDPEWNWFAESLETAAALTRTGHDVRLVVGDGGHSPNHGGVLLPDALRWLWR"
     gene            complement(1340..2176)
                     /locus_tag="VN041_16470"
     CDS             complement(1340..2176)
                     /locus_tag="VN041_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30677.1"
                     /translation="MHRLLPLSGQTALVTGVSRRKGIGFAVARELASLGADVFIHHFR
                     PHDTAQPWGGDDLDAVRSGIRDALAPGAVTADQHADLRDAQEIETLLDAATALTGALD
                     ILVCNQALSGSDGSILEMTADRLDAHWRTNARASLLLTAGFARRKRDAGASAPFDAPT
                     GRVIWMTSGQGDGPMRDEVAYATSKAGLAGVTRTVAAELLDLGIVLNTINPGPVNTGY
                     LDPETTDRDLSWLPGFLETTPFGRFGRPEDPARLIGWLCSDAGSWVVGQVLSSDGGFA
                     LR"
     gene            2351..>3383
                     /locus_tag="VN041_16475"
     CDS             2351..>3383
                     /locus_tag="VN041_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWU30678.1"
                     /translation="MSVDPIPNDPWAAEAERAQRAPGFRRPDLGPVPKPSIYLLGVLA
                     ASRAVGLVLIAEAVARGVGALAGSGITPQATRIILLLGLLGILLRVGGEWATSVVARR
                     IATTVKRDLRSRLWRRIVLGGMDQGGGDSGGGTAVLAADGLDDLDDYYVQTLPALIAV
                     AVVPLIVGVRILGADWLSALIIVLTIPLVPLFMILIGKHTQQRTDAALTALTRLADHL
                     TELARGLPVLVGLGRVDEQTRALDSIQQRYRARTEETLRWAFLSSLALELIATISVAL
                     VAVVLGLRLLDRSMTLEPALVALILAPECFQAMRDVGAAFHASQDGLSALDRARGLLA
                     MPRPRGARTE"
BASE COUNT          461 a         1200 c         1220 g          502 t
ORIGIN      
        1 acccggctgc atcctcgcgg cgtcgccgtc gtgcggacca cgccgggagg accgctcccg
       61 ttcgcagacg gcgggttcga cctcgtcacc agccggcacc cggtctcgcc ggattggcag
      121 gagatcacgc gcgtcctggc cccgggcggc acctacctcg cgcagcacgt gggtcccgcg
      181 agcggattcg aactgatcga gtacttcctc ggcccgctcc cgagcgagcg ccagggccgc
      241 gatccgcagg tcgaggcgga tgccgcgcgc gccgccggac tggagatcgt cgacctgcgg
      301 accgcccggc tgcggatcga gatcagggac atcggcgcgg tcgtgtacct gctgcgtaag
      361 ctggtctggt gggtgccggg attcacggtc gagaagtatc gcgatcgcct tcgtgacctg
      421 cacgagcaga tccagcggga cggcgtgttc gaggatcact cctcgcggca cctcatcgag
      481 gcccgcaggc tctgacggcg ctcagcgcca gagccagcgc agggcatccg gaagcagcac
      541 gccgccgtgg ttcgggctgt gcccgccgtc gccgacgacc agtcgcacgt cgtgcccggt
      601 gcgggtgaga gcggccgcgg tctccagcga ctcggcgaac cagttccact ccgggtcgtt
      661 ccagccgatg tcccggtgcg cggcgtgcag cacgatgcgc agatcgcgtc gctcgccgcc
      721 gctcagcagc cgagggtagg gattgccctg cggcatctgc gcgaagctgg ggttgaacgc
      781 gatgacgcgt cggaagacct ccggccgctg ccagccggcc gtgaaggccg cgtcgccgcc
      841 cgagctcccc ccgcagatgc cgtggccgtc cggctcgagg gtgtgcagcg cggcgacccg
      901 cggcagcacc tcgtcgatga ggaacccggc atagcggtcg tcgtcggcgt cgtactcgac
      961 gttgcggttc ttcggttcat cggatgccgt gcggatgccc gggtcgacga agacacccac
     1021 cacagacggg atcgcgccct gagcggcgag gttgtccagc acgactccgg cgcgcacatc
     1081 gccctcgggg tcgagatacc accagccgtc gttgaagagc atcacggatg ccggccgcag
     1141 cgggtcgtgg ccggccggcg catgcaccca gaccgagcgg gtcgtgcccg ggtaggcggt
     1201 gctgtcgtcg atcgtgaacc ggtcgatccg gccgggttcg acgccgggac gcgggaacga
     1261 gtccggaccg taggcgtagc tcaccgctcc ctggtcgagg gggagcgagg tgtagggctg
     1321 cagggccatg gcatcctcct catcgcaggg cgaagccgcc gtcgctgctg agcacctgac
     1381 cgacgaccca cgaacccgca tccgagcaca gccacccgat cagacgggcg gggtcctccg
     1441 gccggccgaa ccttccgaac ggggtggtct ccaggaaacc cggaagccag ctcaggtcgc
     1501 ggtcggtggt ctccgggtcg aggtagccgg tgttcacggg ccccgggttg atcgtgttca
     1561 gaacgatgcc gaggtcgagg agctcggcgg cgaccgtgcg ggtcacgcct gccagaccgg
     1621 ccttgctggt cgcgtaggcg acctcgtcgc gcatcggacc gtccccctga cccgaggtca
     1681 tccagatcac gcggccggtg ggcgcgtcga agggggcgga tgctccggca tcccgcttcc
     1741 ggcgcgcgaa cccggccgtg agcagcaggc tcgcccgcgc gttcgtccgc cagtgcgcgt
     1801 cgagccggtc ggccgtcatc tcgaggatgc tgccgtccga tccgctcagg gcctggttgc
     1861 acaccaggat gtcgagggcg ccggtcagcg ccgtcgccgc gtcgagcagc gtctcgatct
     1921 cctgcgcgtc ccgcagatcg gcgtgctggt cggccgtgac ggcgccgggt gcgagcgcat
     1981 cgcggatgcc gctgcgcacg gcatcgaggt cgtctccgcc ccacggctgc gcggtgtcgt
     2041 gcggacggaa atggtggatg aagacgtcgg cgccgagcga ggcgagttcg cgggcgacgg
     2101 cgaagccgat gcccttgcgg cgggagacgc cggtgacgag tgcggtctgt ccggaaaggg
     2161 gcagcaggcg gtgcatggtg ggtccttcgg tcgaggatgc cgcggcacac cggagggcgg
     2221 catgacggcg gggacggagg cgactcacct gcactgcatg ccgccaccgt agcatcctgc
     2281 gcactgaacg cgtcgtccgg ggcggaacga tcgggcgcga ccactcttct acaggcggta
     2341 gaattcaggg gtgagcgtcg atccgatccc gaacgatccc tgggcggccg aggccgagcg
     2401 ggcgcagcga gcgcccggat tccgccgacc cgatctcggg ccggttccga agccatcgat
     2461 ctacctgctg ggagtgctgg ccgcctcgcg tgccgtcggg ctcgtgctca tcgccgaggc
     2521 cgtcgcgcgc ggtgtcgggg cactcgcggg gtccggcatc accccgcagg ccacccggat
     2581 catcctcctg ctcgggctcc tcggcatcct gctgcgcgtc ggaggcgagt gggcgacctc
     2641 ggtcgtcgca cggcgcatcg ccaccaccgt caagcgcgac ctgcggagcc ggctgtggcg
     2701 acgcatcgtc ctcggcggga tggaccaggg cggcggtgac tccggcggcg gcaccgccgt
     2761 gctggccgcc gacgggctcg acgacctcga cgactactac gtgcagaccc tccccgcgct
     2821 gatcgcggtg gccgtggtgc cgctcatcgt cggtgtgagg atcctcggcg cggactggct
     2881 cagcgcgctc atcatcgtgc tcaccatccc gctggtgccg ctgttcatga tcctgatcgg
     2941 caagcacacc cagcagcgga cggatgctgc gctcaccgcg ctcacccggc tcgccgatca
     3001 cctcaccgag ctcgcccgag ggcttcccgt gctggtcgga ctcggccgcg tcgacgagca
     3061 gacgcgcgcc ctcgacagca tccagcagcg ctatcgcgcc cgcaccgagg agaccctgcg
     3121 ctgggcgttc ctgtcgtcgc tcgcgctgga gctcatcgcc acgatctcgg tcgccctcgt
     3181 cgccgtcgtc ctcggtctgc ggctgctcga ccgcagcatg acgctggagc cggcgctggt
     3241 cgctctgatc ctcgcgccgg agtgcttcca ggccatgcgc gatgtggggg cggcgttcca
     3301 cgcctcccaa gacggcctgt ccgccctcga ccgcgcccgg ggcctgctgg cgatgccccg
     3361 cccgcgcggg gcccggacgg aag
//
LOCUS       DATMSX010000514         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_159551,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000514 DATMSX010000000
VERSION     DATMSX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159551"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VN041_16480"
     CDS             complement(<1..212)
                     /locus_tag="VN041_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30679.1"
                     /translation="MNITTPHVLVSGAGIAGLALALQLIRGGLRTTVVERAEAPRPAT
                     GTLRVQRWLPDSETMEKARALSKDAPA"
     gene            318..884
                     /locus_tag="VN041_16485"
     CDS             318..884
                     /locus_tag="VN041_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30680.1"
                     /translation="MGNREDLLAGARKVIFERGVAKATARDIAGAAGVSLAAIGYHFG
                     SKEQLITEALTEALGTGIGDRMDAVLHEKSSLPPLEAFAAVWDDMPTAFAENREALIA
                     SLENVVRMLRSPDASKEFASAISGAYAGTAEQLRATRPELDEAQADAVAKLYFTLSQS
                     LGILWLVAPESLPTGEELARAVAALASP"
     gene            complement(995..1273)
                     /locus_tag="VN041_16490"
     CDS             complement(995..1273)
                     /locus_tag="VN041_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30681.1"
                     /translation="MSASEQSEKQSSAQHITTKTGRILRVTDEQLAAAAAAKAAASAI
                     PDPARRKDVLFRVRREEGHELSSWWMIGAFLLTASIVVALLSGVPGGA"
     gene            complement(1401..2387)
                     /gene="glpX"
                     /locus_tag="VN041_16495"
     CDS             complement(1401..2387)
                     /gene="glpX"
                     /locus_tag="VN041_16495"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWU30682.1"
                     /translation="MVSLTADLSPLRPDRNLAMELVRATEAAAIRAVPFIGRGAKEAA
                     DGAAVDAMRAFLGTVDFRGRVVIGEGEKDNAPMLFNGEEVGTGVGPECDIAVDPIDGT
                     SLTAAGRQNALSVLAVSDRGSMLDASSVFYMDKLVTGPAGVGVVDIRLPIGENVRRLA
                     KALDKPVEEMVVSVLHRPRHEQLIQEIRDAGAGTRLMSDGDVAGGINAARHNARTDMC
                     VGIGGSPEGIVTACAIKALGGHIQGILWPRDDEERQRGIDAGLKMDTVYEADDLVKGN
                     NTIFVATGVTDGQLVRGVRREGRYLYTESIVLRSASGTLRRITSDHLVSKWL"
     gene            2571..3599
                     /gene="fbaA"
                     /locus_tag="VN041_16500"
     CDS             2571..3599
                     /gene="fbaA"
                     /locus_tag="VN041_16500"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWU30683.1"
                     /translation="MPVATPEQYAEMLDRAKAGGFAYPAFNVSSSQTINSVLQGLTEA
                     GSDGIIQVTTGGADYFAGHTVKGRATGALAFARFATEVAKNYPITVALHTDHCPKDAL
                     AGFVEPLIAASEEEVKAGRNPIFQSHMWDGSAVPLAENIEIAKELLPRMKAINAILEV
                     EIGVVGGEEDGVAHEGSNDALYTTFGDVDQAVQALGLGEQGRYIAALTFGNVHGVYKP
                     GGVKLRPELLGDIQAQVAEKYGTGKNPLDLVFHGGSGSSDAEIATAVANGVIKMNVDT
                     DTQYAYTRAIADYMFKNYDGVLKVDGEVGNKKQYDPRAWGKIAETAMAARVIESTRQL
                     GSYGQSKS"
     gene            complement(3666..4205)
                     /locus_tag="VN041_16505"
     CDS             complement(3666..4205)
                     /locus_tag="VN041_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30684.1"
                     /translation="MMLVRAYGHRAHGRHALAIGGIALALLLAGCSGGTSGSGGSGGS
                     GSDSGGYGAPSSQAPEPAASGLGVADSSLGKIVVDGEGMTAYMFDKDTQGTKTSACTG
                     ECLAKWPPILSDSATPKADGVTGTLATIDTPDGKHQVTLDGWPLYTFAGDAAKGDVNG
                     QGVGGIWWVLSPAGEKIGG"
     gene            complement(4279..>4642)
                     /locus_tag="VN041_16510"
     CDS             complement(4279..>4642)
                     /locus_tag="VN041_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30685.1"
                     /translation="AAVVVATVVSVALLMPRPAEAVALEPVGGAPVVATVALTTVPWG
                     TRLDVVCAYEGYSGWSGTYELAVTSRDGTTTTLSNWNVQSGATARLSAGTGLPESDIR
                     SVEIRDAAGKVVVKHEFG"
BASE COUNT          740 a         1611 c         1564 g          727 t
ORIGIN      
        1 gccggggcgt cctttgacaa ggcgcgcgcc ttttccatcg tttcgctgtc gggcagccag
       61 cgctggacgc gcaatgtgcc ggtggcgggc ctgggcgcct ctgcccgttc gaccaccgtc
      121 gtgcggagac cgccgcggat cagttgcagt gccagggcga ggccggcaat gccggctccc
      181 gagacaagga cgtggggcgt ggtgatgttc atgtcgtgat tcctctccgc gttagacatt
      241 tgtctgacac aactgtttta cacaggactc atacgaatgt ctagtacgca cgtctaaatc
      301 atggctagga tggctccatg ggaaatcgag aggacctgtt ggccggcgcc cggaaggtga
      361 tcttcgagcg cggcgtcgca aaggcgaccg cgcgcgacat cgccggcgca gccggggtca
      421 gcctggcggc gatcggctac cacttcggct cgaaggagca gctgatcacc gaagccctca
      481 ccgaggcgct cgggacgggc atcggcgaca ggatggacgc cgtgctccac gagaaatcct
      541 cgctgccgcc gctcgaggcg ttcgccgcgg tgtgggacga catgcccacc gccttcgctg
      601 agaaccgcga ggccctgatc gccagcctcg agaacgtggt gcggatgctg cgctcccccg
      661 atgcgtcaaa ggagttcgcg tccgcgatat ccggggccta cgcaggtacc gcagaacagc
      721 tccgcgcgac gcggcccgaa ctcgacgagg cccaggcgga tgccgtcgcg aagctgtact
      781 tcacgctctc gcagagcctc ggcattctgt ggctggtcgc cccggagtcg ttgccgacag
      841 gcgaggaact cgctcgcgcc gtggccgctc tcgcctcgcc gtgacgtcgt tggccgcgtg
      901 catcatgggg atatgaatgc gtcggccgtc gtcagacatc ggcgagcttc gcgcccgcct
      961 cgatcacggc agtgagttga accggaggcc gggctcaggc gccgccgggc actcccgaca
     1021 gcagggcgac gacgatgctc gcggtcagca ggaaggcgcc gatcatccac cacgagctga
     1081 gttcgtggcc ctcttcgcga cgcacgcgga acagcacgtc cttgcggcgg gccggatcgg
     1141 gaatggcact ggccgcggcc ttcgccgcgg cggctgcagc gagctgctca tcggtgacgc
     1201 gcaggatacg accggtcttc gtggtgatgt gctgagcgct gctctgcttc tcggactgct
     1261 cgctcgctga catcccgagc tctcctgttc tgaccaactc cagcgcgctg agggcgccgg
     1321 atccacactc attgtgccag ccgcggctgg gaatcgcggg atgggcgggc caccgcggtc
     1381 agaacgttac ctcgtcgtgc tcacagccac ttcgacacga ggtggtcgga cgtgatgcgg
     1441 cgcagcgtgc ccgaggcgga gcgcagcacg atgctctcgg tgtagagata gcgtccctcg
     1501 cgacgcacgc ctcggaccag ctggccgtcg gtcacgccgg tggcgacgaa gatcgtgttg
     1561 ttgcccttga cgaggtcgtc ggcctcgtac acggtgtcca tcttcagtcc ggcgtcgatg
     1621 ccgcgctgcc gctcctcgtc gtcgcgcggc cagaggatgc cctggatgtg cccgccgagc
     1681 gccttgatcg cgcatgcggt gacgatgccc tccgggctgc cgccgatgcc gacgcacatg
     1741 tcggtgcggg cgttgtgacg agcggcgttg atgcctcctg cgacgtcgcc gtcgctcatc
     1801 agccgggtac cggcgccggc atcccggatc tcctggatca gctgctcgtg acgcgggcgg
     1861 tgcagcaccg agacgaccat ctcctcgacc ggcttgtcga gcgccttggc gaggcggcgg
     1921 acgttctcgc cgatgggcag ccggatgtcg acgacgccga cgcccgcggg gccggtgacg
     1981 agcttgtcca tgtagaagac gctggacgcg tcgagcatcg agccccggtc ggagaccgcg
     2041 agcacggaca gcgcgttctg gcggcccgcc gcagtcaggg aggtgccgtc gatcgggtcg
     2101 accgcgatgt cgcactcggg gccgacgccg gtgccgacct cctcgccgtt gaacagcatc
     2161 ggggcgttgt ccttctcgcc ctcgccgatc acgacacggc ctcggaagtc gaccgtgccg
     2221 aggaacgcgc gcatggcgtc gacggcggcg ccgtcggccg cctccttggc gccgcggccg
     2281 atgaagggca ccgcccggat cgcggccgct tcggttgcac ggaccagctc catcgcgagg
     2341 ttgcggtcgg gacgaagcgg gctgagatcg gctgtaaggc tcaccatgcg gtcagcctag
     2401 tgatccggcc gtgcggaaca ggcgagtttt cccgttttcc cgacgaagga ttcgacgttc
     2461 ttaacaggtc ttcaagatcc gcggcccgac gccgcgaaac agccgagaag aaccggcatc
     2521 cgcgcggata gagtgagcat gacccccgca catcagaagc aggagaatcc atgcccgtcg
     2581 ccactccgga acagtacgca gagatgctcg accgcgcgaa ggccggtggc ttcgcgtacc
     2641 ccgctttcaa cgtctccagc tcgcagacga tcaactctgt gctgcagggt ctgaccgagg
     2701 ccggctccga cggcatcatc caggtcacca ccggtggcgc ggactacttc gccgggcaca
     2761 ccgtcaaggg ccgtgccacg ggtgccctcg ccttcgcgcg cttcgcgacc gaggtcgcca
     2821 agaactaccc catcaccgtg gcgctgcaca ccgaccactg cccgaaggac gcgctggccg
     2881 gcttcgtcga gccgctcatc gccgcctccg aggaggaggt caaggccgga cgcaacccga
     2941 tcttccagtc gcacatgtgg gacggctcgg ccgtgccgct cgctgagaac atcgagatcg
     3001 ccaaggagct gctgccccgc atgaaggcga tcaacgcgat cctcgaggtg gagatcggcg
     3061 tcgtcggcgg cgaggaggac ggcgtcgcgc acgagggctc caacgacgcc ctctacacga
     3121 ccttcggtga cgtcgaccag gccgtgcagg cgctgggcct gggtgagcag ggccggtaca
     3181 tcgccgccct caccttcggc aacgtgcacg gcgtctacaa gccgggtggc gtcaagctgc
     3241 gccccgagct gctgggcgac atccaggcgc aggtcgccga gaagtacggc accggcaaga
     3301 acccgctcga cctggtcttc cacggcggat cgggctcctc ggacgctgag atcgcgaccg
     3361 ccgtcgccaa cggcgtcatc aagatgaacg tcgacaccga cacccagtac gcgtacacgc
     3421 gtgcgatcgc cgactacatg ttcaagaact acgacggcgt gctcaaggtg gacggcgagg
     3481 tcggcaacaa gaagcagtac gacccgcgcg cctggggcaa gatcgccgag accgccatgg
     3541 cggcccgcgt gatcgagtcc acccgccagc tcggctcgta cggccagtcc aagagctgac
     3601 acgcacgaag aagagtggat gccccggatc gtcctgatcc ggggcatccg tcgtcccgcg
     3661 ccgggtcagc cgccgatctt ctcaccggcg ggcgagagca cccaccagat gccgccgacg
     3721 ccctgaccgt tcacgtcgcc cttcgccgcg tcgcccgcga aggtgtacag cggccagccg
     3781 tccagcgtga cctggtgctt gccgtcgggg gtgtcgatcg tggcgagcgt gcccgtgacg
     3841 ccgtcggcct tcggcgtcgc ggagtcggac aggatcggcg gccacttcgc caggcactcg
     3901 ccggtgcagg cgctggtctt cgtgccctga gtgtccttgt cgaacatgta ggcggtcatg
     3961 ccctcgccgt ccaccacgat cttcccgagc gagctgtccg cgacgccgag gccggatgcc
     4021 gccggctccg gggcctgcga cgacggcgcc ccgtatccgc cggagtccga accggaaccg
     4081 ccggaacctc cggagccgct cgtgccgccg gagcagccgg cgaggaggag tgcgagggcg
     4141 atgcccccga tggcgagcgc gtgacgcccg tgtgccctgt gcccgtatgc cctgaccaac
     4201 atcatgccgt ccctttcgtc tgagcagcct ccgcgatggc ggctgttccc gtgacacgag
     4261 accgacaggg cggatggttc agccgaactc gtgcttcacg acgactttcc cggcggcatc
     4321 ccggatctcg acggaccgga tgtcggactc cggcagcccc gtgcccgccg acagtcgtgc
     4381 cgtggccccg gattgcacgt tccagttcga cagcgtggtg gtggtgccgt cccgcgaggt
     4441 gaccgccagc tcgtaggtgc cgctccatcc cgaatacccc tcataggcgc acaccacgtc
     4501 gagcctggtg ccccatggca cagtcgtgag cgccaccgtc gccacgacgg gcgctccccc
     4561 gacgggctcc agcgccacgg cctcagccgg gcggggcatc agcagtgcga ccgacaccac
     4621 ggtggccacg acgaccgcgg cc
//
LOCUS       DATMSX010000515         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_121856,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000515 DATMSX010000000
VERSION     DATMSX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121856"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VN041_16515"
     CDS             complement(<1..1571)
                     /locus_tag="VN041_16515"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWU30686.1"
                     /translation="MTTQPSLPPVLRPADPPRRALAELADRFATEVRGDLAGVQISGI
                     TLATADLRAGEAFVAIQGVNRHGAEFAQTAADQGAVAVITDAAGADIAASAGLPIVIV
                     DDPRGVLGELSAWVYGTGASDPLPLLFGTTGTNGKTSVSHLLQGIFDQLGVVSGLSST
                     AERHIAGEVIVSRLTTPEASEMHALLALMRERNVEAVAVEVSAQALSRHRVDGIVFDV
                     AGFTNLSHDHLDDYADMEEYFQAKLPLFTPERARRGVISLDSAYSPRVVEAASIPVVT
                     VGTPSIAADPDAAAAADWVVTIDDERQAGTVFTLTGRDGRTLTTMVPVIGQHMAANAG
                     LAIVMLLEAGYDWDRITAAFERDGQILAYLPGRTQFISGETGPAVYVDFGHSPDAFEK
                     TLAAVRRVTPGKVLMLFGADGDRDATKRYDMARTAVEGSDILVVTDHHPRFEDPDSIR
                     ATLVAGARKARPDAEIHEFSPPEKAIVAAVGMVGEGDAILWAGPGHQDYRDIRGVRTP
                     YSARELARRALIAAG"
     gene            complement(1658..2335)
                     /locus_tag="VN041_16520"
     CDS             complement(1658..2335)
                     /locus_tag="VN041_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HWU30687.1"
                     /translation="MSHTLRRGAIAAATLAVFLIAAPTAASAHVGVSPDRIPAGQVTP
                     LTFSFSHGCGESPTTSLRVAMPKGIGSAWPAFDGDWAADVEKDAGGAITAVTFTAAQP
                     VPVDLRGSVELAVITDKNAADELVFPVEQRCVSGTNEWTQVAKDGENAHDLDSPAPVV
                     ALTAADASGDHGQESAHGQHTAAPEASSSSSEQPAGILPMLLGGGGLVLALAALVVSI
                     RAFRRRA"
BASE COUNT          360 a          825 c          961 g          355 t
ORIGIN      
        1 cagccggcgg cgatgagcgc gcgccgggcg agctcgcgcg ccgagtacgg ggtgcggacg
       61 ccacggatgt cgcgatagtc ctggtggccc ggaccggccc agaggatcgc atcgccttcg
      121 ccgaccatgc cgacagccgc cacgatcgcc ttctccggcg gggagaactc gtggatctcg
      181 gcatccggtc gcgctttgcg cgctccggcg acgagcgtcg cgcggatgga gtccgggtct
      241 tcgaagcgcg ggtgatggtc cgtgacaacc aggatgtcgc tgccctcgac ggccgtgcgg
      301 gccatgtcgt agcgcttggt ggcgtcgcgg tcgccgtcgg cgccgaacag catcagcacc
      361 ttgcccggcg tgacccggcg gaccgcggcg agggtcttct cgaaggcgtc cggggagtgg
      421 ccgaagtcga cgtacacggc ggggccggtc tcgccggaga tgaactgggt gcggccgggg
      481 aggtacgcca ggatctggcc gtcgcgctcg aacgccgcgg tgatgcggtc ccagtcgtat
      541 ccggcctcga gcagcatgac gatcgcgagt ccggcattcg cggccatgtg ctggccgatc
      601 accggcacca tggtcgtcag cgtgcgtccg tcgcggccgg tgagcgtgaa caccgtgccc
      661 gcctgccgtt cgtcgtcgat ggtgacgacc cagtcggccg ctgcggcggc atccgggtcg
      721 gcggcgatcg acggagtccc gacggtgacg acggggatgc tcgcggcctc gacgacgcgg
      781 gggctgtacg ccgagtccag gctgatcacg ccgcggcggg cgcgctcggg cgtgaacagc
      841 ggcagcttcg cctggaagta ctcctccatg tcggcatagt cgtcgaggtg gtcgtgcgag
      901 aggttcgtga agccggcgac gtcgaacacg atgccgtcca cgcggtggcg ggagagggcc
      961 tgcgcactga cctccacggc gacggcctcg acgttccgct cgcgcatcag cgccaggagg
     1021 gcgtgcatct cggatgcctc gggggtggtg agccgggaga cgatgacctc accggcgatg
     1081 tgccgctccg cagtggaaga gaggcccgag acgacgccca gctggtcgaa gatgccctgc
     1141 agcaggtgcg agacgctggt cttgccgttg gtgccggtgg tgccgaacag cagcggcagc
     1201 ggatccgatg ccccggtgcc gtacacccac gcgctcagct cgcccagcac gccgcgagga
     1261 tcgtcgacga tcacgatcgg cagacccgct gatgccgcga tgtccgcgcc cgcggcgtcc
     1321 gtgatgaccg cgacagcgcc ctgatcggcg gcggtctgcg cgaactcggc accgtggcgg
     1381 ttcacgccct ggatcgcgac gaaggcctcc ccggcgcgga gatcggcggt ggcgagggtg
     1441 ataccgctga tctgaacgcc cgcgagatca ccgcggacct ccgttgcgaa gcggtctgcg
     1501 agctccgcca gagcgcgacg gggtgggtcg gcggggcgga gcacgggcgg cagtgaaggc
     1561 tgagtggtca tagcgtcatc catgatctca cgcacagcgg agggatcctg cggattggag
     1621 gcgcccggcg tgcgccggac gcctccgaca gggtgggcta tgcgcggcgc cggaaggcgc
     1681 ggatggacac cacgagcgcc gccagggcga ggaccagtcc gccgccgccc aggagcatcg
     1741 gcaggatccc ggccggctgc tcggagctcg acgacgatgc ctccggagcg gccgtgtgct
     1801 gaccgtgcgc gctctcctgg ccgtggtcgc ctgaggcgtc cgcagcggtc agcgcgacga
     1861 cgggagccgg cgagtccaga tcgtgggcgt tctcgccgtc cttcgccacc tgggtccact
     1921 cgttcgtccc ggagacgcag cgctgctcga cggggaacac cagttcgtcg gcggcgttct
     1981 tgtccgtgat cacggccagc tccaccgaac cgcgcagatc gaccggcacg ggctgcgcgg
     2041 cggtgaacgt gacggcggtg atcgccccgc ccgcatcctt ctctacgtct gccgcccaat
     2101 cgccgtcgaa cgccggccag gcgctgccga tgcccttcgg catcgcgacg cgcagcgacg
     2161 tggtcgggga ctcgccgcag ccgtggctga acgagaaggt cagcggagtg acctggccgg
     2221 cggggatccg gtccggtgag acgcccacgt gcgcggaggc cgcggtgggt gctgcgatga
     2281 ggaacacggc gagggtggcc gcggcgatgg cgccgcgacg gagggtatga gacatggatt
     2341 cgtccttcat ggatgagacg ccgcgcacgc gcgcagcgag aagatgctcc gcgcgtgagt
     2401 gcgcggacgg atgccgtcgc ggggcgcgat cggcgggacg cggtccggtg gccggacggc
     2461 gatgacgggc tggatgcccg gagggtcagc tgagcgctga c
//
LOCUS       DATMSX010000516         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_178585,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000516 DATMSX010000000
VERSION     DATMSX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178585"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /gene="metE"
                     /locus_tag="VN041_16525"
     CDS             <1..1108
                     /gene="metE"
                     /locus_tag="VN041_16525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWU30688.1"
                     /translation="KVFGAVRARALSEADFSRDSYADREAAQLALDLPALPLTTIGSF
                     PQTADIRRARAQFGRGEVPQDDYEAFLKSEIDRVVALQEDLGLDVLVHGEAERNDMVQ
                     YFAENLDGFAVTENGWVQSYGSRATRPSILWGDVSRPAPITVSWASYAQSLSTKHVKG
                     MLTGPVTILAWSFVRDDQPLGETANQVALALRDEITDLEAAGIAIIQVDEPALRELLP
                     LKKADQPAYLDWSVNSFRLATGGAAAATQVHTHLCYSEFGVVIDAIRALDADVTSIEA
                     ARSRMEVVADIARYGFDHGVGPGVYDIHSPRVPSVDEIESLLRRAVDELPLRQLWVNP
                     DCGLKTRGYDETVASLRNIVEATRRVREDVVVPA"
     gene            complement(1193..2998)
                     /locus_tag="VN041_16530"
     CDS             complement(1193..2998)
                     /locus_tag="VN041_16530"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWU30689.1"
                     /translation="MPTMTASDDPARTWLAAFEDALLARDVARAAGLFAATSFWRDLI
                     AFSWNITTVENPAGVADLLTATLERTAPHGFRFTEEPETADGVTSAWFEFETAVGRGR
                     GLVRIVDEDGPKAWTLLTTMYELKGHEEPLRERRPKGAEHGARRDRVSWLERRQAEEA
                     SLGTETQPYALVIGGGQGGIALGARLRQLGVPALVIDRYPRPGDQWRGRYKSLCLHDP
                     VWYDHLPYIKFPENWPVFAPKDKVGDWLESYVKVMEVPYWSNTTATSAAFDEASGTWT
                     VEIERDGAPLTLHPTQLVFATGMSGKPNVPVFPGQDVFRGEQQHSSQHPGPDAYSGKK
                     VVVIGSNNSAFDICGALWEHGADVTMVQRSSTHIVKSDTLMDIGLGDLYSERALAAGV
                     TTEKADLIFASLPYRIMHEFQIPLYERMRERDKDFYDRMTAAGFDLDWGDDGSGLFLK
                     YLRRGSGYYIDVGSAELVADGEVALAHGDVDHLTEDAVVLKNGTVLPADLVVYATGYG
                     SMNGWVADLISQEVADTVGKCWGLGSDTTKDPGPWEGEQRNMWKPTQQPNLWFHGGNL
                     HQSRHYSLYLALQLKARHAGIDTPVYGLQQVHHLR"
     gene            3108..>3803
                     /locus_tag="VN041_16535"
     CDS             3108..>3803
                     /locus_tag="VN041_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWU30690.1"
                     /translation="MATSPDLADLARRLVRVHDAVLSGSAPPQHPREVVARSWRRTMS
                     LGVDADAVRRAEPIAASALEDLRRRSRLSDVIDELRAPLLAAADASSYLVVVTDADGL
                     VLWRSGAPRVKLHADRLGFVDGARWTESVVGTNAIGTALVEDAPVQLFSAEHYENAQV
                     PWYCAAEPIHDPIDGTLLGVVDVSGPALTLHPAIRALVSAAARIAEASLWRRHSMRLE
                     RLRRAAQPLLAGVQ"
BASE COUNT          533 a         1320 c         1350 g          600 t
ORIGIN      
        1 gaaagttttc ggcgccgtgc gtgcccgcgc tctctcggag gccgacttct cccgcgactc
       61 ctacgcggac cgtgaggccg cccagctggc gctggacctg cccgcgctgc cgctgaccac
      121 catcggctcg ttcccccaga ccgccgacat ccgtcgcgcc cgcgcgcagt tcggccgcgg
      181 cgaggtgccc caggacgact acgaggcgtt cctgaagtcg gagatcgacc gcgtggtcgc
      241 gctgcaggag gacctggggc tggacgtgct cgtgcacggc gaggccgagc gcaacgacat
      301 ggtgcagtac ttcgccgaga acctggacgg cttcgcggtg accgagaacg gctgggtgca
      361 gtcctacggc tcgcgcgcca cgcgcccgtc gatcctgtgg ggcgacgtgt cgcgtccggc
      421 gccgatcacc gtctcctggg cgtcgtacgc ccagtcgctg agcaccaagc acgtcaaggg
      481 catgctcacc ggtcccgtca cgatcctggc ctggtcgttc gtgcgcgacg accagccgct
      541 cggggagacc gccaaccagg tcgccctcgc cctgcgcgac gagatcaccg acctcgaggc
      601 cgccggcatc gcgatcatcc aggtcgacga gccggccctg cgggagctgc tgccgctgaa
      661 gaaggccgac cagcccgcct acctggactg gtccgtgaac tccttccgtc tggccacggg
      721 cggcgctgcg gcggccacgc aggtgcacac gcacctctgc tactcggagt tcggcgtcgt
      781 gatcgacgcg atccgcgcgc tggacgccga cgtcacctcg atcgaggccg cgcgcagccg
      841 catggaggtc gtcgccgaca tcgccaggta cggcttcgac cacggcgtgg gccctggcgt
      901 ctacgacatc cactcaccgc gggtgccgag cgtcgacgag atcgagtcgc tgctgcgccg
      961 cgccgtcgac gagctgccgc tgcgtcagct ctgggtcaac ccggactgcg gtctgaagac
     1021 ccgcgggtac gacgagaccg tcgcgtcgct gcgcaacatc gtcgaggcga cgcgccgcgt
     1081 ccgcgaggac gtcgtcgtcc cggcctgaga cgcgtccccg cagggcctgc acaagccctg
     1141 cgcaggtacg gatgggcccc gctccgtgga ggagcggggc ccatccgggt cttcagcgca
     1201 ggtgatgcac ctgctgcagg ccgtacaccg gggtgtcgat gccggcgtgc ctggccttca
     1261 gctgcagcgc caggtacaac gagtagtgcc gcgactggtg caggttgccg ccgtggaacc
     1321 agaggttcgg ctgctgcgtc ggcttccaca tgttgcgctg ctcgccctcc cagggcccgg
     1381 ggtccttggt cgtgtccgag ccgagccccc agcacttgcc gacggtgtcg gcgacctcct
     1441 ggctgatgag gtctgccacc cagccgttca tcgagccgta gccggtggcg tagacgacca
     1501 gatccgccgg gagcacggtg ccgttcttca gcaccacggc atcctcggtg agatggtcga
     1561 cgtcgccgtg cgcgagtgcg acctcgccgt cggcgaccag ctcggcggag cccacgtcga
     1621 tgtagtagcc ggagccgcgt cgcaggtact tcaggaacag gcccgagccg tcgtcgcccc
     1681 agtcgaggtc gaatccggcc gccgtcatcc ggtcgtagaa gtccttgtcg cgttcgcgca
     1741 tccgttcgta cagcgggatc tggaactcgt gcatgatccg gtagggcagc gacgcgaaga
     1801 tgagatcggc cttctcggtg gtgacgccgg cggcgagagc acgctcggag tacaggtcgc
     1861 cgagaccgat gtccatcagg gtgtcgctct tgacgatgtg cgtcgaggag cgctgcacca
     1921 tggtcacatc ggcgccgtgc tcccacagcg ccccgcagat gtcgaaggcg gagttgttgc
     1981 tcccgatcac gacgaccttc ttgccgctgt aggcgtcggg accgggatgc tgggacgagt
     2041 gctgctgctc gccgcggaac acgtcctggc cggggaagac cggcacgttc ggcttgcccg
     2101 acatgcccgt cgcgaagacc agctgggtcg gatgcagagt cagcggggcg ccgtcgcgct
     2161 cgatctcgac ggtccaggtg ccggaggcct cgtcgaaggc cgcggaggtc gcggtcgtgt
     2221 tcgaccagta cggcacctcc atgaccttca cataggactc gagccagtcg ccgaccttgt
     2281 ccttcggggc gaagacgggc cagttctcgg ggaacttgat gtacggcagg tggtcgtacc
     2341 agaccgggtc gtgcaggcag agcgacttgt agcgcccgcg ccattgatct ccagggcgcg
     2401 ggtaccggtc gatcacgagc gcagggacgc cgagctgacg cagccgggcg ccgagcgcga
     2461 tgccgccctg tccgccgccg atgacgagcg cgtagggctg ggtctcggtg cccagcgacg
     2521 cctcctcggc ctgccggcgc tccagccatg acacgcggtc gcgccgggcc ccgtgctcgg
     2581 cgcccttcgg gcgtcgttcg cgcaggggct cctcgtgtcc cttgagctcg tacatggtgg
     2641 tcagcagcgt ccacgccttc gggccatcct cgtcgacgat ccggacgaga ccccggccac
     2701 ggcccacggc ggtctcgaac tcgaaccagg cggacgtcac tccgtcggcg gtctcgggct
     2761 cctcggtgaa ccggaagccg tgcggggcgg tgcgctcgag cgtggcggtg aggaggtcgg
     2821 cgacgcctgc ggggttctcg accgtggtga tgttccagga gaaggcgatc aggtcgcgcc
     2881 agaaactggt cgccgcgaag agcccggcgg cgcgcgcgac atcgcgggcg agcagggcat
     2941 cctcgaaggc cgccagccag gtgcgggccg gatcgtcgga tgcggtcatg gtcggcatcg
     3001 tcgccgaccg ctcatcgagt tgggtcatgc gtacagtgca ctcggccgcg ctcgcgccgt
     3061 gaagcgttgc acggtgttgc aacgtggccc gggatacgct ccccgccatg gcaacgtcgc
     3121 ctgatctcgc agacctcgcc cgccgtctcg tgcgtgtgca cgatgccgtg ctctccggca
     3181 gcgcgcctcc gcagcatccg cgggaggtgg tcgcgcggtc gtggcgccgc acgatgagcc
     3241 tgggcgtgga tgccgatgcc gtgcgccgcg ccgagccgat cgccgcctcg gcgctggagg
     3301 atctgcggcg tcgttcgcgg ctgtccgacg tgatcgacga gctgcgcgcc cctctgctcg
     3361 ccgccgccga cgcgtcgtca tacctcgtcg tcgtgacgga tgccgacgga ctggtgctgt
     3421 ggcgcagcgg cgcgccgagg gtgaagctgc acgccgatcg gctcggcttc gtggacggtg
     3481 cgcgatggac ggagagcgtc gtggggacca acgcgatcgg caccgcgctc gtcgaggatg
     3541 cgcccgtgca gctgttctcg gccgagcact acgagaacgc gcaggtgccc tggtactgcg
     3601 ccgccgagcc gatccatgac ccgatcgacg ggaccctgct cggcgtcgtg gatgtcagcg
     3661 gccctgcact gacgctgcat cccgccatcc gcgcgctggt gtcggcggca gcgaggatcg
     3721 ccgaggcgtc gctctggcgc cggcacagca tgcggctgga acggctgcga agggccgcgc
     3781 agcctctgct ggccggcgtg cag
//
LOCUS       DATMSX010000517         5630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_8403,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000517 DATMSX010000000
VERSION     DATMSX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8403"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VN041_16540"
     CDS             complement(<1..245)
                     /locus_tag="VN041_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HWU30691.1"
                     /translation="MTEARMTFDERYRAISARDTRFDGQFVTAVRSTGIYCRPSCPAR
                     TPKPANVTFYPTSAAAHEAGYRACKRCLPEATPGSPA"
     gene            complement(319..1236)
                     /locus_tag="VN041_16545"
     CDS             complement(319..1236)
                     /locus_tag="VN041_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HWU30692.1"
                     /translation="MRAASAQAPNAVVLIRPHHFRPNPQTAADNTFQSEADHDVSREA
                     YDACTRVADALRAEGVRVHLFEDETDKHPDSVFPNNWFSTHAGGRVAVYPMYAPNRRG
                     ERRADVIEMLKSEYRVQEVIDYSGLEPDDLFLEGTGAMVLDHVSRIAYAVRSHRCDPV
                     LVERFCTVFGYEPVVFDAADPDGVPIYHTNVMMCIGTDIALIGLDSIVGDVRREQIAQ
                     RIRDTGRTLVPLTAEQVASFAGNAIELRDHNGERILVMSQTARRSLTEEQVRVISASC
                     RILALDVAPIELAGGSARCMIAGIHLDPR"
     gene            complement(1233..2270)
                     /locus_tag="VN041_16550"
     CDS             complement(1233..2270)
                     /locus_tag="VN041_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HWU30693.1"
                     /translation="MVRFVDVPNMRRWLVDTGIERAIEGIAETLVEDFKRWEDFDKTP
                     RVASHSHIGVIELMPASDGRQYGFKYVNGHPSNPARGLQTVTAFGVLADVDSGYPTLW
                     SEMTILTALRTAATSAMVARVLAPEGATTMAMIGTGSQSEFQALAFRSLLGIQSLRIW
                     DTDPHAMDVFEANMRPLGFDIVRAASGAEAVQGAQIITTCTADKANATVLTDDMVGPG
                     VHVNAIGGDCPGKTELDARILHRGDIFVEYPPQTRIEGEIQQLDPEYPVIELWRVLRG
                     EAAGRTSAEQITIFDSVGFAIEDFSALRYLDSAIDGTDYFTEVDIIAEPADPKNLFAL
                     VAEGASAHSLA"
     gene            2394..2825
                     /locus_tag="VN041_16555"
     CDS             2394..2825
                     /locus_tag="VN041_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU30694.1"
                     /translation="MSSLDDLDRRLLSLLRANSRESVVNLARRLGVTRATVDSRLKRL
                     VDSGVIVGFSVRVRDPAAASVVRAVMLIAVEGRNTREVIRSLRGVPQIAALHTTNGRW
                     DLVAEISCDGLASFDETLGTIRGIDGIRDSETSILLSSATV"
     gene            complement(2947..4554)
                     /gene="purH"
                     /locus_tag="VN041_16560"
     CDS             complement(2947..4554)
                     /gene="purH"
                     /locus_tag="VN041_16560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWU30695.1"
                     /translation="MAGPRLDPSLYRHRDLVPIRRALVSVSDKTGLVELASALAEAGV
                     EIVSTGSTAQTVRDAGFEVTDVAAVTGVAEMLDGRVKTLHPKIHGGLLADLRLEDHER
                     QLADLGIEPFELVVVNLYPFVETVASGAEPDAIVEQIDIGGPAMVRAAAKNHANVAIV
                     VSPESYPAIIESVKGGGTDLAQRRELAARAFSHTATYDTAVAQWFSEGTLSDGADLPA
                     HLTIKAERLETLRYGENSHQRAAIYTRTGGHGIAQATQLQGKEMSYNNYVDADAALRA
                     AYDMVLPSVAIIKHANPCGIATTAPNALDPIASAHLRAHECDPVSAYGGVIAANGTVT
                     LKMAENLKDIFTEVIVAPDFEPAALEVFKAKKNLRLLKLPEDWQQERMDVRLVSGGLL
                     LQDADRFPDDIVSVAKDWELVSGERPDEAAMENLIFAWKACRAVKSNAIVLAKDNATV
                     GVGMGQVNRVDSCRLAVERAGARAAGAVAASDAFFPFADGPQVLLDAGITAIVQPGGS
                     VRDGEVIDAARKAGVTMFFTGERHFFH"
     gene            complement(4592..5167)
                     /gene="purN"
                     /locus_tag="VN041_16565"
     CDS             complement(4592..5167)
                     /gene="purN"
                     /locus_tag="VN041_16565"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWU30696.1"
                     /translation="MLTLAVLISGTGSNLRALLEAASHPDFPARVVAVGADRDADGLA
                     HAEEFGIPTFTVPYSAYPSREAWGEELAEQLDAWQPDLIVLSGLMRLLPAAVVARYAP
                     RIVNTHPAYLPEFPGAHGVRDALAAGVEQTGASVIVVDDGVDSGPILAQERVAVLPDD
                     TEATLHERIKPVERRLLIDVVERIATGDLVL"
     gene            complement(5172..>5630)
                     /locus_tag="VN041_16570"
     CDS             complement(5172..>5630)
                     /locus_tag="VN041_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HWU30697.1"
                     /translation="VSPAGTSVGVVPGIPVLGLVPANSSMWMLVAVLLPIACGALAGW
                     IVRSRLVWERVGTGIWPRAAIAVGIAAVSAGVVALAAVLASGSIGPGRLSEVGPAVGP
                     FALAIGIEVLIGAGILLLTPRHRDELAEERTDRWREEMGGDVSDLTAPVD"
BASE COUNT          829 a         1910 c         2032 g          859 t
ORIGIN      
        1 cacgcgggcg agccgggcgt cgcctcgggc aggcaccgct tgcacgcgcg gtaccccgcc
       61 tcgtgcgccg ccgcactggt cggatagaac gtgacgttcg cgggcttcgg cgtgcgcgca
      121 gggcagctcg gacggcagta gatgccggtc gagcgcaccg ccgtcacgaa ctgtccgtcg
      181 aagcgcgtgt cacgcgcact gatcgcgcgg taccgctcgt cgaaggtcat cctggcttcg
      241 gtcatgcctc catgctgtca cgtccgaatg ccgccggctg gcggaaatcg gacacggcag
      301 tgacgcagcc cgagtgcgtc agcgcgggtc gaggtggatg ccggcgatca tgcagcgcgc
      361 cgaccctccg gccagctcga tcggcgcgac gtccagcgcc aggatgcggc acgaggccga
      421 gatcacccgc acctgctcct cggtgaggct gcggcgggcg gtctgggaca tcacgaggat
      481 gcgctcgccg ttgtggtcgc gcagttcgat cgcgttgcct gcgaacgagg ccacctgctc
      541 ggcggtgagc ggcaccagcg tgcggccggt gtcgcggatg cgctgggcga tctgctcgcg
      601 gcgcacgtcg ccgacgatgc tgtccagtcc gatcagggcg atgtcggtgc cgatgcacat
      661 catcacgttg gtgtggtaga tcggcacgcc gtcggggtcc gcggcatcga agaccacggg
      721 ctcgtagccg aacaccgtgc agaaccgctc cacgagcacc gggtcgcagc ggtgcgagcg
      781 cacggcatag gcgatgcgcg acacgtggtc gagcaccatc gcccccgtcc cctccaggaa
      841 caggtcgtcg ggctccaggc cggagtagtc gatgacctcc tgcacccggt actcggactt
      901 gagcatctcg atcacgtcgg cccggcgctc gccgcggcgg ttcggcgcgt acatcgggta
      961 caccgcgacc ctgccaccgg cgtgcgtgga gaaccagttg ttggggaaca cgctgtcggg
     1021 atgcttgtcg gtctcgtcct cgaacaggtg cacgcgcacg ccctcggccc gcagcgcgtc
     1081 ggccacgcgc gtgcacgcgt catacgcctc gcgcgacacg tcgtggtcgg cctcgctctg
     1141 gaaggtgttg tcggcagccg tctgcgggtt gggccggaag tggtggggcc ggatcagcac
     1201 caccgcgttc ggggcctgag cggatgccgc tctcacgcca ggctgtgcgc cgaggcgccc
     1261 tcggcgacca gcgcgaagag gttcttcggg tcggccggct cagcgatgat gtcgacctcg
     1321 gtgaagtagt cggtgccgtc gatcgcggag tccaggtagc gcagcgcgga gaagtcctcg
     1381 atcgcgaagc cgaccgagtc gaagatcgtg atctgctcgg ccgaggtgcg ccctgcggcc
     1441 tcaccacgga gcacgcgcca cagctcgatg accgggtact cggggtccag ctgctggatc
     1501 tcaccctcga tgcgggtctg cggcgggtac tccacgaaga tgtcgccgcg gtgcaggatg
     1561 cgggcgtcga gctcggtctt gcccgggcag tcgccgccga tcgcgttcac gtgcacaccc
     1621 gggccgacca tgtcgtccgt cagcacggtg gcgttggcct tgtctgcggt gcaggtggtg
     1681 atgatctgcg cgccctgcac ggcctccgcg ccgctcgcgg cgcggacgat gtcgaagccg
     1741 agtgggcgca tgttggcctc gaacacgtcc atggcgtgcg ggtcggtgtc ccagatgcgc
     1801 aggctctgga tgccgagaag cgagcggaac gccagtgcct ggaactcgga ctggctgccc
     1861 gtgccgatca tggccatcgt cgtggcgccc tcgggcgcga gcacgcgggc gaccatcgcc
     1921 gaggtggcgg cggtgcgcag cgcggtgagg atggtcatct cggaccacag cgtcgggtag
     1981 ccgctgtcca cgtcggccag cacgccgaac gcggtgacgg tctgcaggcc gcgcgcaggg
     2041 ttgctcgggt ggccgttgac gtacttgaag ccgtactggc ggccgtcact ggcgggcatc
     2101 agctcgatca cgccgatgtg ggagtggctg gcgacgcggg gggtcttgtc gaagtcctcc
     2161 cagcgcttga agtcttcgac gagcgtctcg gcgatcccct cgatcgcgcg ctcgataccg
     2221 gtgtcgacca gccagcggcg catgttcgga acatcgacaa aacggaccat cgtccatccc
     2281 ttcactcttc gtccctccaa tctacgcgcc tcgggtgtaa ctttcattca ccatcgtgag
     2341 tcattttcgc aatcgagcgc gcattgtgag catccatagg gtacattctg ggcatgagtt
     2401 ctctcgatga tctcgaccgc cgcctgctgt cgctgctgcg cgccaacagc cgcgagtcgg
     2461 tcgtgaacct cgcccggcgc ctcggagtga cccgcgccac cgtcgacagc cggctcaagc
     2521 ggctcgtcga cagcggcgtg atcgtcggct tctcggttcg cgtccgcgac cccgccgcgg
     2581 catccgtggt gcgcgcggtc atgctcatcg ccgtggaggg gcgcaacacc cgcgaggtga
     2641 tccgcagcct gcggggcgta ccccagatcg ccgcgctgca caccacgaac gggcggtggg
     2701 acctggtcgc cgagatcagc tgcgacggtc tggcgtcctt cgacgagacg ctcggcacca
     2761 tccgcggcat cgacggcatc cgcgacagcg agaccagcat cctgctgagc tcggcgacgg
     2821 tctgagcccg cagcacccgc cgacaccccg ggaaatcgcc gagaccccgc gctatggacg
     2881 tccatggcac ggggtctcga cggcaaggcg gggtgtcggc gaaggccgac gggtccccgc
     2941 gtgggctcag tggaagaagt ggcgctcccc cgtgaagaac atcgtcacgc cggccttgcg
     3001 ggcggcgtcg atgacctcgc cgtcgcgcac cgacccaccg ggctgcacga tggccgtgat
     3061 gcccgcgtcc agcagcacct gcggtccatc tgcgaacggg aagaacgcgt cggatgccgc
     3121 gaccgccccg gccgcgcgtg cgcctgcgcg ctccaccgcg aggcggcagg agtcgacgcg
     3181 gttgacctgg cccatgccca cgccgacagt ggcgttgtcc ttggcgagca cgatcgcgtt
     3241 cgacttgacc gcgcggcacg ccttccacgc gaagatgagg ttctccatgg cggcctcgtc
     3301 gggacgctca cccgagacga gctcccagtc cttggcgacc gagacgatgt cgtccgggaa
     3361 gcgatcggcg tcctgcagca gcaggccgcc cgagaccagg cgcacatcca tgcgctcctg
     3421 ctgccagtcc tcgggcagct tgagcagacg caggttcttc ttggccttga acacctcgag
     3481 cgcagcgggc tcgaagtcgg gcgcgacgat gacctcggtg aagatgtcct tgaggttctc
     3541 ggccatcttc agcgtgaccg tgccgttcgc ggcgatcacg ccgccgtacg cggacaccgg
     3601 gtcgcactcg tgagcgcgca ggtgcgcgct ggcgatcggg tcgagcgcgt tcggcgcggt
     3661 cgtggcgatg ccgcacgggt tggcgtgctt gatgatcgcg accgagggca gcaccatgtc
     3721 gtaggcggcg cgcagagcgg catccgcgtc gacgtagttg ttgtacgaca tctccttgcc
     3781 ctgcagctgc gtggcctggg cgatgccgtg gccgccggtg cgggtgtaga tcgcggcgcg
     3841 ctggtgcgag ttctcgccgt agcgcagggt ctccaggcgc tccgccttga tcgtcaggtg
     3901 cgcgggcagg tcggcgccgt cgctgagggt gccctcagag aaccactggg cgacggcggt
     3961 gtcataggtc gcggtgtgcg agaacgcgcg ggctgcgagc tcacggcgct gggcgaggtc
     4021 ggtgccgccg cccttcaccg actcgatgat cgccgggtac gactccggcg agacgacgat
     4081 ggccacgttg gcgtggttct tggccgcggc gcgcaccatg gccgggccgc cgatgtcgat
     4141 ctgctcgacg atcgcgtcgg gctcggcgcc cgaggcgacg gtctccacga acgggtagag
     4201 gttcaccacg accagctcga agggctcgat gccgaggtcg gcgagctgcc gttcgtggtc
     4261 ctccaggcgc aggtcggcga gcaggccgcc gtggatcttg ggatgcaggg tcttcacgcg
     4321 tccgtcgagc atctcggcca caccggtgac ggcggccacg tcggtgacct cgaaaccggc
     4381 gtcccgcacc gtctgcgcgg tcgagccggt ggagacgatc tccacgcccg cctcggcgag
     4441 agcggaggcg agctcgacca gaccggtctt gtcgctcacc gagaccagcg cgcgcctgat
     4501 cggcaccagg tcgcggtgac ggtacagcga gggatcgagg cgggggccgg ccatggtgat
     4561 gctccttcag acgtgcgttg tgagggggtg gtcagagaac gaggtcgccg gtggcgatgc
     4621 gctccacaac gtcgatcagc agccgccgct cgacgggttt gatgcgctcg tgcagcgtgg
     4681 cttcggtgtc gtcgggcagc accgcgacgc gctcctgggc gaggatgggg ccgctgtcga
     4741 caccgtcgtc gacgacgatg acgctcgcac cggtctgctc gacgccggcg gccagagcgt
     4801 cgcgcacacc gtgcgcgccg gggaactcgg gcaggtaggc cgggtgcgtg ttgacgatac
     4861 gcggcgcata gcgcgccacg acggcggcgg gcagcaggcg catgagcccg ctcagcacga
     4921 tgaggtcggg ctgccaggca tccagctgct ccgccagctc ctcaccccag gcctcccgcg
     4981 acgggtaggc ggagtacggc acggtgaagg tcgggatgcc gaactcctcg gcgtgcgcca
     5041 ggccgtcggc gtcgcggtcg gcgcccaccg ccaccacgcg tgcagggaag tcggggtggc
     5101 tcgcggcctc gagcagggct cggagattcg agccggtacc ggagatgagt acggcgagcg
     5161 tcagcacccg gtcagtctac cggggcggtc aggtcggaga cgtcgccgcc catctcctcg
     5221 cgccagcggt cggtccgctc ctcggcgagc tcgtcgcggt ggcgaggggt gaggagcagg
     5281 atgccggcgc cgatcagcac ctcgatgccg atcgccagcg cgaaaggacc gacggcaggg
     5341 ccgacctcgg acagccgccc cggcccgatg gacccggatg ccagcaccgc ggcgagcgcg
     5401 accacgcctg cggagacggc ggcgatgccg acggcgatcg ccgcgcgggg ccagatcccg
     5461 gtgccgacgc gctcccagac caggcgggac cgcacgatcc agccggccag cgcaccgcac
     5521 gcgatcggga ggagcaccgc cacgagcatc cacatcgacg agttcgcagg cacgaggccg
     5581 agcacgggga tgccggggac cacgcccacg ctggtgcccg cgggcgacac
//
LOCUS       DATMSX010000518         5718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_178593,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000518 DATMSX010000000
VERSION     DATMSX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178593"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VN041_16575"
     CDS             <1..610
                     /locus_tag="VN041_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWU30698.1"
                     /translation="AVAGGGDWWFGNMHKWPCAPRGSALLVTQAHDRQELWPLIDSWG
                     APESFPGRFDTQGTIDATTYLSTPAAIDFIEQEIGWAAAREAMTGMADAGAEAIAEAL
                     RPYSDTDPLTPLPSPVPSMRLIRLPQGLGATREDADELRMQLLDEVGVETAFTSFRGV
                     GYYRLSVHLYTEGSDFEAFVERCVPQLLRRAGIGIRSSASIS"
     gene            649..1962
                     /locus_tag="VN041_16580"
     CDS             649..1962
                     /locus_tag="VN041_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWU30699.1"
                     /translation="MKKRILTVAAGIGTVAALTLTGCSSGGAPAADGTVTLQMVESLT
                     NPARTKLIRSMLDDFQKENPKIKVNLVSPPTEQADQKIQQMLQSGRGVDVLEVRDITV
                     GPFSNNGWLYDLKSDLDKWDGWDDLTDNAKSASVAEDGKTYFVPYGFYGLSLFYRTDL
                     VEQAGFDGPPHSWTDLLEQASKIQDPGKNIYGYAFRGGQNANSNVVAAIEAYTIDDLN
                     VDDAFLLKDGKTMFSAPEAQQAVDDYFDLFKKASPPSAVSWGYPEMVAGFTNGSTAFL
                     LQDPEVIATVQSSSLKADQWDTAPLLVGPSGKAAQPLAVAGWGVAQKSANKEAATKLV
                     EFLSSKEPATKFAQANSLVPIIASAADDDFYKTGPWSSYVTMTKDPETYVNVRQPRGV
                     SWWTEWIQKSDQDVQKVLLGNMSTSELLKSWDEFWTEKYASQKKG"
     gene            1966..2904
                     /locus_tag="VN041_16585"
     CDS             1966..2904
                     /locus_tag="VN041_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30700.1"
                     /translation="MTSTHSKGGSAGTAGGASRRRPFRSRQALTLLAFMLPAIVFVCW
                     FTYWPMLQGARMAFHDWNLWDLTSTPWVGFDNFVAVFQDPLFPQVAWNSVLWVVGSLV
                     PQLVIGFLIALALRKRFRFRGLYQALVFFPWAVSGFLIGMLFRWMFNAEFGVVNDLLM
                     KAGLISQPLPWLADPKLAMFAVITANIWYGVTFFAIMILAALQSVPDEVLEAASLDGA
                     GKARQLFSIIIPYISVTLMLTILLRVIWIFNFPDIIWAMTNGGPAKQTHIITTWMIDY
                     TQQGNYGIASAIGLVVVAILFVFCAFYLVAMRKVQR"
     gene            2901..3809
                     /locus_tag="VN041_16590"
     CDS             2901..3809
                     /locus_tag="VN041_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30701.1"
                     /translation="MTSTMTSPINATRRLTVPNPASAPRGPRTVTVGGVVRVVGLALW
                     LIITLFPLYWIALTSFKAPGTINRYPIEYWPSEPSLANYVSLFQDSAFGVFLGNSALV
                     ALTAGAVATLIALLSAYVIARFEFRGKGAVLIAFLLTQMIPAFIALGPLYSMMSDLGL
                     VDTKPGLILVYIAICIPFSTVMLRGFFENVPDALEEAAMIDGCSRLGALFRVLVPVMT
                     PGIIAAFIFNFVNCWNELFLSVVLMNTDENRTVPSALNGFISTFNIDWGSMSAAAVLT
                     ILPTMLMFALASRWIVAGLTSGAVKE"
     gene            complement(3864..4877)
                     /locus_tag="VN041_16595"
     CDS             complement(3864..4877)
                     /locus_tag="VN041_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HWU30702.1"
                     /translation="MVNYRYLGNSGLKVSEITYGNWVTHASQVGDDAAVATVHAALDA
                     GITTFDTADTYANTAAEVVLGKALAGQRRAGLEIFTKVYFPTGPKGPNDTGLSRKHIR
                     DSIDGSLQRLGVEYVDLYQAHRYDHETPLEETMQAFADVVRQGKALYIGVSEWTAEQL
                     REGHALARELGVQLISNQPQYSMLHRVIEGKVVPASEELGISQIVWSPMAQGVLSGKY
                     LPGQPVPDGSRATDPHSGAHFIQRFLQDDILAAVQNLKPIAAEAGLTMPQLAIAWVLQ
                     NPNVAAALVGASRPEQLADNVKASGVKLDAEVMTAIDTALAGVVFDDPEDTYTVSPKE
                     RLV"
     gene            4936..5595
                     /locus_tag="VN041_16600"
     CDS             4936..5595
                     /locus_tag="VN041_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30703.1"
                     /translation="MPTLPRLLYVEDEAETAAIVVEVLSDEYLVDHARTGERGLDLAL
                     GQRYDVMVVDRRLPGMTGAELLQAVRTAHITTPAIMLTALGTVADRVSGLDAGADDYL
                     LKPFDFDELRARLRALRRGRGPGTRREIGDWLFSTTSLALYGPDGGRVPLTQAEADLL
                     ELLTSSPEHVFSREEIVASLFPGGSAGTVDAYVHYIRRKSTPEIIDTVRARGYRSGMP
                     R"
BASE COUNT          899 a         1949 c         1909 g          961 t
ORIGIN      
        1 cgccgtcgcc ggcggcggcg actggtggtt cgggaacatg cacaagtggc cgtgcgcccc
       61 gcgcggctcg gcgctgctgg tcacccaggc gcacgaccgc caggagctgt ggccgctgat
      121 cgactcgtgg ggagcgcccg aatcgttccc gggtcgcttc gacacgcagg gcacgatcga
      181 cgccaccacc tacctcagca cgcccgcggc gatcgacttc atcgagcagg agatcggctg
      241 ggcggccgcc cgcgaggcca tgacaggcat ggcggatgcc ggggcggagg cgatcgccga
      301 ggcattgcgc ccgtacagcg acaccgatcc gctgacgccg ctcccctcgc cggtgccgtc
      361 gatgcgcctg atccgcctcc cccagggcct gggcgccacc cgcgaggacg ccgacgagct
      421 gcgcatgcag ctgctggacg aggtgggcgt cgaaacggcg ttcaccagct tccgcggagt
      481 cggctactac cgcctgtcgg tgcacctcta caccgagggc tccgacttcg aggcgttcgt
      541 cgagcggtgc gtgccgcagc tgctccggcg ggccggcatc ggcatccgct cctcggcatc
      601 catctcctga cagacgcaac atcccacacc ccgattgaga ggcacaacat gaagaaaagg
      661 atcctgacgg tcgccgcagg catcggcacc gtcgccgccc tcaccctgac cggctgctcc
      721 tccggaggcg cccccgccgc cgacggcacc gtcacgctgc agatggtcga gagcctgacc
      781 aacccggccc gcaccaagct gatccgctcg atgctggacg acttccagaa ggagaacccg
      841 aagatcaagg tcaacctggt ctcgcctccc accgagcagg ccgaccagaa gatccagcag
      901 atgctgcagt ccggtcgcgg cgtcgacgtg ctcgaggtgc gcgacatcac ggtcgggccg
      961 ttctcgaaca acgggtggct gtacgacctg aagagcgacc tcgacaagtg ggacgggtgg
     1021 gacgacctga ccgacaacgc caagtccgca tcggtcgccg aggacggcaa gacctacttc
     1081 gtgccctacg gcttctacgg gctgtcgctg ttctaccgca ccgacctggt cgagcaggcc
     1141 ggcttcgacg gcccgccgca cagctggacc gacctgctcg agcaggcgtc caagatccag
     1201 gacccgggca agaacatcta cggctacgcg ttccgcggcg gccagaacgc caacagcaac
     1261 gtggtcgccg ccatcgaggc gtacacgatc gacgacctga acgtcgatga cgcgttcctg
     1321 ctgaaggacg gcaagacgat gttctccgcc ccggaggcgc agcaggcggt cgacgactac
     1381 ttcgacctgt tcaagaaggc ctcaccgccc tccgccgtct cctggggcta ccccgagatg
     1441 gtggccggct tcaccaacgg ctcgaccgcg ttcctcctgc aggaccccga ggtgatcgcc
     1501 accgtgcagt cctcgtcgct caaggccgac cagtgggaca ccgccccgct tctcgtgggt
     1561 cccagcggca aggccgccca gccgctggcc gtcgccgggt ggggcgtcgc gcagaagagc
     1621 gccaacaagg aggccgcgac caagctggtc gagttcctct cgtcgaagga gcccgcaacg
     1681 aagttcgccc aggcgaacag cctggtgccg atcatcgcat ccgccgcgga tgacgacttc
     1741 tacaagacgg gcccctggag cagctacgtc accatgacga aggacccgga gacctacgtc
     1801 aacgtgcgtc agccccgcgg cgtcagctgg tggaccgagt ggatccagaa gtcggaccag
     1861 gacgtgcaga aggtgctgct cggcaacatg tcgacgagcg agctgctgaa gtcctgggac
     1921 gagttctgga cagagaagta cgcgtcccag aagaagggct gagtcgtgac gagcacgcac
     1981 tcgaagggag gttccgccgg cacggccggc ggggcctccc ggcgccgtcc gttccgctcc
     2041 cggcaggcgc tgacgctgct ggcgttcatg ctgcccgcga tcgtgttcgt ctgctggttc
     2101 acgtactggc cgatgctgca gggcgcacgg atggcgttcc atgactggaa cctgtgggat
     2161 ctgacctcca caccgtgggt cggattcgac aacttcgtcg cggtcttcca ggatccgctg
     2221 ttcccgcagg tggcgtggaa ctcggtgctc tgggtggtcg gatccctggt cccccagctg
     2281 gtgatcggat tcctgatcgc gctcgcgctg cgcaagcgct tccggttccg cggcctctac
     2341 caggcgctgg tgttcttccc ctgggcggtg tcgggattcc tgatcgggat gctgttccgc
     2401 tggatgttca acgccgagtt cggcgtcgtc aacgacctgc tcatgaaggc cggtctcatc
     2461 tcgcagccgc tgccctggct ggccgatccg aagctggcga tgttcgcggt gatcaccgcc
     2521 aacatctggt acggcgtgac gttcttcgcc atcatgatcc tggcggctct gcagtcggtc
     2581 cccgacgaag tgctggaggc tgcgagcctc gacggcgccg gcaaggcccg ccagctgttc
     2641 tcgatcatca tcccgtacat ctcggtcacg ctgatgctga cgatcctgct gcgggtgatc
     2701 tggatcttca acttcccgga catcatctgg gcgatgacca acggcggtcc tgccaagcag
     2761 acgcacatca tcaccacctg gatgatcgac tacacgcagc agggcaacta cggcatcgcg
     2821 agcgcgatcg gactggtcgt cgtcgccatc ctgttcgtgt tctgcgcgtt ctacctggtc
     2881 gcgatgagga aggtgcagcg atgacctcca cgatgacgtc tccgatcaat gcgacacggc
     2941 gcctgaccgt gccgaacccg gcatccgctc cccgcggtcc acgcacggtg accgtcggcg
     3001 gcgtggtgcg cgtcgtcggg ctggcgctct ggctgatcat cacgctgttc ccgctgtact
     3061 ggatcgcgct gacctcattc aaggcgccgg gcacgatcaa ccgctacccg atcgagtact
     3121 ggccgagcga gccatccctg gcgaactacg tgagcctgtt ccaggacagc gccttcggtg
     3181 tcttcctggg caactccgcg ctggtcgcgc tgaccgcggg ggccgtggcc acgctgatcg
     3241 cgctgctgag cgcctacgtg atcgcacgtt tcgagttccg cggcaagggc gcggtgctga
     3301 tcgcattcct gctcacgcag atgatccccg cgttcatcgc gctgggaccg ctgtactcga
     3361 tgatgagcga cctgggcctg gtcgacacca agcccggtct gatcctggtg tacatcgcta
     3421 tctgcatccc gttctcgacg gtcatgctgc gcggcttctt cgagaacgtg ccggacgcgc
     3481 tcgaggaggc ggcgatgatc gacggatgct cgcgcctggg cgcactgttc cgggtgctgg
     3541 tcccggtgat gacgccgggc atcatcgccg cgttcatctt caacttcgtg aactgctgga
     3601 acgagctgtt cctgtcggtg gtgctgatga acaccgacga gaaccgcacg gtgccgtcgg
     3661 cactgaacgg cttcatctcg acgttcaaca tcgactgggg ctcgatgtcg gcggcggccg
     3721 tgctgacgat cctgccgacc atgctgatgt tcgctctggc gagccggtgg atcgtcgccg
     3781 gactgacgtc gggtgctgtg aaggagtgat gcaggactgc ccgggggcgc ttcaggcgaa
     3841 gcacccccgg gcagtcggat gcctcagacc aggcgctcct tcggggagac ggtgtaggtg
     3901 tcctcggggt cgtcgaacac gacgccggcg agcgcggtgt cgatggcggt catcacctcg
     3961 gcgtccagct tcacgccgga ggccttgacg ttgtcggcga gctgctcggg acgcgaagcg
     4021 ccgaccaggg ccgcggccac gttcgggttc tgcagcaccc aggcgatggc gagctgcggc
     4081 atcgtcaggc ccgcctcggc cgcgatcggc ttgaggttct gcacggccgc gaggatgtcg
     4141 tcctgcagga agcgctggat gaagtgcgca cccgagtgcg ggtcggtggc gcgcgagccg
     4201 tcaggcaccg gctgaccggg caggtacttg ccgctgagca cgccctgcgc catcggcgac
     4261 cagacgatct gcgagatgcc cagctcctcc gatgcgggca cgaccttgcc ctcgatgacg
     4321 cggtgcagca tggagtactg cggctggttc gagatgagct gcacgcccag ctcgcgagcc
     4381 agcgcgtggc cttcgcgcag ctgctcggcg gtccactccg agacgccgat gtacagcgcc
     4441 ttgccctggc gtacgacgtc ggcgaaggcc tgcatggtct cctcgagcgg ggtctcgtgg
     4501 tcgtagcggt gcgcctggta caggtcgacg tactccacgc cgaggcgctg cagcgagccg
     4561 tcgatcgagt cgcggatgtg cttgcggctc aggccggtgt cgttcgggcc cttcgggccg
     4621 gtcgggaagt acaccttggt gaagatctcg aggcctgcgc ggcgctgacc ggcgagcgcc
     4681 ttgccgagca cgacctcggc tgcggtgttg gcgtaggtgt ccgcggtgtc gaacgtggtg
     4741 atgccggcat ccagtgcggc gtgcacggtg gcgacagcgg catcgtcacc gacctgcgag
     4801 gcgtgcgtga cccagttgcc gtaggtgatc tccgagacct tgagaccgct gttgccgaga
     4861 tagcgatagt tgaccatggg acaacgctat cctcctccca gcctggcaag ggccgcgctc
     4921 tgtcacactg ttcccatgcc caccctgccg cggctgctgt acgtcgagga cgaggccgag
     4981 accgctgcga tcgtcgtgga ggtgctctcg gacgagtacc tcgtcgacca tgcgcgcacg
     5041 ggcgagcgcg ggctggacct cgcgctcgga cagcggtacg acgtgatggt cgtggaccgg
     5101 cgcctccccg gcatgacggg tgccgagctg ctgcaggccg tgcgcaccgc ccacatcacg
     5161 accccggcga tcatgctcac cgcattgggc acggtggccg accgggtgag cggcctggat
     5221 gccggggccg acgactatct gctcaagccc ttcgacttcg acgagctgcg cgcacggctg
     5281 cgcgcgctcc ggcggggacg cgggccgggg acgcgccggg agatcggcga ctggctgttc
     5341 tccacaacct ccctggcgct ctacggcccc gacggcggac gtgtgccgct cactcaggcc
     5401 gaggccgacc tgctcgagct gctgacctcc agccccgagc acgtgttctc gcgcgaggag
     5461 atcgtcgcga gcctcttccc gggcggctcg gcggggaccg tcgatgccta cgtgcactac
     5521 atccggcgca agtccacgcc cgagatcatc gacaccgtcc gcgcgcgcgg ctatcgcagc
     5581 ggaatgccgc gatgagcgcc cgcaccgacc acgccgtcgc cgccgatcgc acgcgggtgc
     5641 agcgctccgc actgcgcaca ggcctgtggg tcggcatcgc atccgctgcc gtggtgaccg
     5701 cggtgacggc cacgatca
//
LOCUS       DATMSX010000519         4263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_178611,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000519 DATMSX010000000
VERSION     DATMSX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178611"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VN041_16605"
     CDS             complement(<1..421)
                     /locus_tag="VN041_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWU30704.1"
                     /translation="MALVMGVDSSTQSCKVVIVDAVSGAIVRTGRASHPDGTSVDPEA
                     WWEALQAAIADAGGMEDVGAWAVGGQQHGMVALDSSGAVVRDALLWNDTRSAGAASDL
                     IAEFGASALAHRTGLVPVASFTIAKLRWLRDAEPANAE"
     gene            complement(475..1665)
                     /gene="xylA"
                     /locus_tag="VN041_16610"
     CDS             complement(475..1665)
                     /gene="xylA"
                     /locus_tag="VN041_16610"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HWU30705.1"
                     /translation="MSLTPTKADKFSFGLWTIGYNGADPFGGPTRPQLDVVHAVEKLA
                     ELGAYGLTFHDDDLFAFGSTDAERQTQIDRLKGALADTGLIVPMVTTNLFSAPVFKDG
                     GFTSNDRQVRRFALRKVLRQLDLGAELGAKTFVMWGGREGAEYDSAKDVRAALERYRE
                     AVNLLGDYVTDKGYDIRFAIEPKPNEPRGDILLPTLGHALAFIDSLERPELVGLNPEV
                     GHEQMAGLNFAAGIAQALYHGKLFHIDLNGQRGIKYDQDLVFGHGDLHNAFALVDLLE
                     NGGANGAPAYDGPRHFDYKPSRTEDEAGVWDSAAANMRMYLLLKERAAAFRADPEVQE
                     ALAAARVAELSQPTLGEGESYDDLLADRSAFEDFDPSAYFGARGFGFVRLQQLASEHL
                     MGAR"
     gene            complement(1742..2740)
                     /locus_tag="VN041_16615"
     CDS             complement(1742..2740)
                     /locus_tag="VN041_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30706.1"
                     /translation="MNRRTTIHDVAHAAGVSVATVSKAINGRDGVAPATLAHVLSVVD
                     ELGYASSLVATSMRRRRTNVIGVLVAEFEPFALQLLSGVSTELQGTRFDVLAYAGSVS
                     AGEHRGWERRSLSRLGGTLIDGAIVVTPTSTPADASVPIVAIDPHAGPDGPATVSVIN
                     VDGARAATEHLISLGHRRIAHLRGRTDLVSAQQREQGYREALDAAGIRFDPALIADGG
                     YRTADSTAGAHALLDLADRPTAVFAANDLSAIEMIRVAGERGLHVPDDLSVIGFDDIP
                     EAAANAPQLSTVRQPLTEMGAVAVRLLLRMLDGEAHEHVRLPSELVVRETTTAPRT"
     gene            2879..4174
                     /locus_tag="VN041_16620"
     CDS             2879..4174
                     /locus_tag="VN041_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30707.1"
                     /translation="MKIGKAWVAAAAAVATLGMLGLTGCSGGSDGAGADGATTLTMWH
                     NSTTGPGKQYWADAAAAFHEENPDVTIKVTSIQNEDMDGKLQTAVNSGDMPDIFMARG
                     GGKLADIVNAGKVKDLTKLIDPDVKKAFGDAPFSAFSVDGKVYGMPSAVLPGGFFYSK
                     DLFAQAGVSSEPTTMDELEAAVTKLKAAGIEPIALGAKAAWPAAHWYYFFALRACGQD
                     LIENLATKADFSDPCWLTAAENLQDFAGTEPFNKGFLTTSPQEGANSSAGLLANHKAA
                     MELMGAWDVGVIASLTPDEKPLPDLGWFPFPEVDGGDGGAGAMMGGVDGYSCSADSPA
                     ACEKFLNFVSSKEWQEKYAVAFQTIPANQDATGAVTDPSLKPLMEAYTKAPYVALWLD
                     TALGQNVGNALNTSVVEMLAGQGSPKKLVDTVTKAEARG"
BASE COUNT          687 a         1450 c         1507 g          619 t
ORIGIN      
        1 gctcggcgtt ggcgggctcc gcgtcgcgca gccagcgcag cttggcgatc gtgaaggacg
       61 ccacgggcac gagtccggtg cggtgcgcca gagcggatgc cccgaactcg gcgatcagat
      121 cggatgctgc gcccgcggaa cgcgtgtcgt tccacagcag cgcgtcgcgg accacggcgc
      181 ccgaggagtc cagcgccacc atgccgtgct gctgcccgcc gacggcccag gcgcccacgt
      241 cctccatgcc gccggcatct gcgatcgccg cctgcagggc ctcccaccac gcctcgggat
      301 cgaccgaggt cccgtccggg tgcgacgcac gccctgtgcg cacgatcgca ccggagacgg
      361 catccacgat cacgaccttg caggactgcg tcgatgagtc gacgcccatc acgagtgcca
      421 tgcggggcat cctctcatct catgttccgg tctcggcgat caggggcgag cgggtcagcg
      481 agcgcccatc aggtgctcgg atgccagctg ctggagtcgc acgaagccga agccacgcgc
      541 gccgaagtac gcggaggggt cgaagtcctc gaaggcgctg cgatcggcga ggaggtcgtc
      601 gtaggactcg ccctcgccca gggtgggctg cgacagctcg gcgacgcgcg cggcggccag
      661 ggcctcctgc acctccgggt cggcgcggaa cgcggcggcc cgctccttca gcagcaggta
      721 catgcgcatg ttggcggcgg cggagtccca cacgcccgcc tcgtcctcgg tgcggctggg
      781 cttgtagtcg aagtgccggg ggccgtcgta cgccggggcg ccgttcgcgc cgccgttctc
      841 gagcagatcc accagcgcga acgcgttgtg caggtcgccg tggccgaaca ccaggtcctg
      901 gtcgtacttg atgccgcgct gcccgttcag atcgatgtgg aacagcttgc cgtggtacag
      961 cgcctgggcg atgccggcgg cgaagttgag ccccgccatc tgctcgtgac cgacctcagg
     1021 gttcagcccg accagctcgg ggcgctccag cgagtcgatg aacgcgagcg cgtgcccgag
     1081 cgtcggcagc aggatgtctc cgcggggctc gttgggcttg ggctcgatgg cgaagcggat
     1141 gtcgtagccc ttgtcggtga cgtagtcgcc gagcaggttc acggcctcgc ggtagcgctc
     1201 gagagcggcc cgcacgtcct tcgcggagtc gtactcggcg ccctcgcggc cgccccacat
     1261 cacgaaggtc ttcgcgccca gctcggcgcc cagatccagt tggcgcagca ccttccgcag
     1321 cgcgaagcgg cgcacctgcc ggtcgttcga cgtgaagccg ccgtccttga agaccggggc
     1381 gctgaacagg ttggtggtga ccatcggcac gatcagcccg gtgtcggcca gggcgccctt
     1441 caggcggtcg atctgggtct gccgctcggc atccgtcgac ccgaacgcga acaggtcgtc
     1501 gtcgtggaag gtgaggccgt aggcgccgag ctcggcgagc ttctcgaccg cgtgcacgac
     1561 gtcgagctgc ggccgggtgg gcccgccgaa cgggtcggcg ccgttgtagc cgatggtcca
     1621 gagtccgaac gagaacttgt cggccttggt gggggtgagt gacatggtga tccgccttca
     1681 gcgacatcga cgaaatgttg tggatcagaa catatcaagt cgatgcggcg gatgcaacgg
     1741 atcaggtcct cggtgcggtc gtcgtctccc gcacgacgag ttcggacggc aggcgcacgt
     1801 gctcgtgcgc ctcgccgtcc agcatccgca gcagcaggcg cacggcgacg gcacccatct
     1861 ccgtcagcgg ctggcgcacc gtgctcagct gcggcgcgtt cgcggccgct tcggggatgt
     1921 cgtcgaagcc gatcaccgag aggtcatcgg gtacgtgcag gccgcgctcg cccgccacgc
     1981 ggatcatctc gatggcggag aggtcgttcg ccgcgaagac cgccgtggga cggtccgcga
     2041 gatcgagcag cgcgtgcgca cccgcggtcg aatccgccgt gcggtagccg ccgtcggcga
     2101 tgagggcggg atcgaagcgg atgcccgcgg catcgagcgc ctcgcgatac ccctgctcgc
     2161 gctgctgggc ggagacgagg tcggtgcggc cgcgcaggtg ggcgatgcgg cggtgcccca
     2221 gtgagatcag atgctcggtc gcggcccgtg cgccgtcgac gttgatcacg ctcaccgtcg
     2281 cgggcccgtc cggaccggca tgcgggtcga tcgcgacgat gggcaccgag gcgtccgcgg
     2341 gggtcgaggt cggcgtcacg acgatggctc cgtcgatcag agttccgccc aggcgggaga
     2401 gcgagcgacg ctcccagccg cggtgctcgc ccgccgagac gctgcccgcg taggccagca
     2461 cgtcgaagcg cgtgccctgc agctcggtgg agaccccgct cagcagttgc agcgcgaacg
     2521 gctcgaactc cgccaccagc acgccgatca cgttggtccg ccgtcgtcgc atcgacgtcg
     2581 ccaccagtga gctggcgtat ccgagctcgt cgaccacgct cagcacgtgc gccagcgtcg
     2641 cgggcgcgac gccgtcgcgg ccgttgatgg ccttggagac ggtggcgacg gagactccgg
     2701 cggcgtgagc gacatcgtgg atcgtcgtgc ggcggttcat ccgtccaatc tagtgatgga
     2761 aaacgatatc gataacgatt gacatggcga atcgaacctg ccagagtggc cggagcacat
     2821 tcggtgtagt cggcaacaaa tggggccgga ccgcgaactc gaagaggaga cacacggcat
     2881 gaagatcggc aaggcatggg tcgccgcagc agcggccgtg gcgacgctgg ggatgctcgg
     2941 gctcaccggc tgctccggcg gctcggacgg agccggcgcc gacggcgcga ccacgctcac
     3001 gatgtggcac aactccacca cgggtcccgg caagcagtac tgggccgacg cggcggcggc
     3061 cttccacgag gagaaccccg atgtgacgat caaggtcacg tcgatccaga acgaggacat
     3121 ggacggcaag ctgcagaccg ccgtcaactc gggcgacatg cccgacatct tcatggcgcg
     3181 cggcggcggc aagctcgccg acatcgtcaa cgcgggcaag gtgaaggacc tcacgaagct
     3241 catcgacccc gacgtgaaga aggccttcgg cgacgccccg ttcagcgcct tcagcgtcga
     3301 cggcaaggtc tacgggatgc cgagcgcggt gctccccggc ggcttcttct acagcaagga
     3361 cctgttcgcg caggcggggg tcagttccga gcccaccacc atggacgagc tcgaggcggc
     3421 cgtcaccaag ctgaaggcgg caggcatcga accgatcgcg ctcggtgcca aggccgcgtg
     3481 gccggccgcg cactggtact acttcttcgc cctgcgagcc tgcggtcagg acctgatcga
     3541 gaacctcgcg accaaggccg acttctccga tccgtgctgg ctcacggcgg ccgagaacct
     3601 gcaggacttc gccgggacgg agccgttcaa caagggcttc ctgacgacct ccccgcagga
     3661 gggagcgaac tcctcggccg gactgctggc caaccacaag gccgccatgg agctgatggg
     3721 cgcgtgggac gtgggcgtga tcgccagtct cacccccgac gagaagcccc tgcccgacct
     3781 gggctggttc cccttccccg aggtggacgg cggcgacggc ggggccggcg cgatgatggg
     3841 cggagtcgac ggctactcct gctcggccga ctccccggct gcgtgcgaga agttcctcaa
     3901 cttcgtctcc tcgaaggaat ggcaggagaa gtacgccgtg gcgttccaga ccatcccggc
     3961 gaaccaggat gccaccggag cggtcaccga tccgtcgctg aagccgctga tggaggcgta
     4021 caccaaggct ccctacgtcg ccctgtggct ggacaccgcc ctcgggcaga acgtcggcaa
     4081 cgcgctgaac acgagcgtcg tcgagatgct cgcggggcag ggctcgccga agaagctcgt
     4141 cgacacggtc accaaggccg aggcgagggg ctgaccggct catgaccgac acccaggcac
     4201 tgcagcgcac gacggtgcct gagaggatcg ccgtccccgg agcgggcccc gcgcccgctc
     4261 cgg
//
LOCUS       DATMSX010000520         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_102550,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000520 DATMSX010000000
VERSION     DATMSX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102550"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..140
                     /locus_tag="VN041_16625"
     CDS             15..140
                     /locus_tag="VN041_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30708.1"
                     /translation="MTLRGARATGSKTSTSALRGLLRDDPRSRSEFLALAADRAR"
     gene            186..1325
                     /locus_tag="VN041_16630"
     CDS             186..1325
                     /locus_tag="VN041_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30709.1"
                     /translation="MTIAIVGGGLAAATAATELREKGYEGDVVIYSAEDHLPYERPPL
                     SKEVLLGSKQVEEAQVHDSDWYADNDVQLERGVKVVSIDPAAHTLRAEPVAGGESTEH
                     SYEKLLLATGASPRHFPAADKVSDPVYLRTVEDNARLLEALKPGAKVLIVGAGWIGLE
                     VASAARQKGAEVTIYEFADLPLVAVLGPEVAQLFADLHRAHGVDLRLSTPVDEAAMAA
                     ADLVVVGIGAVPNTELAEQAGLDVDHGVLVDETLRTSDPDIYAIGDIASQQHPVLGRR
                     IRVEHWDTAIEQGKVAAHNLAGASEQYAHLPYFFTDQYDLGMEYFGSVGPEGYDRVDI
                     EGDTDVANGGAFRAYWVKDGTVRAAMHANDWDSSDAVRDSIGTAR"
     gene            1322..2260
                     /gene="folP"
                     /locus_tag="VN041_16635"
     CDS             1322..2260
                     /gene="folP"
                     /locus_tag="VN041_16635"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWU30710.1"
                     /translation="MTAASAASAVGAEAGTAGDRMLRRIGIRDFDFSRQVAVMAVINR
                     TPDSFYDKGATFALDRAVESALRAGDQGADWVDIGGVPFGRGPVVTTAEEIDRVVPAV
                     AAITAARPELVISVDTNNAEVAAAAIDAGAAVINDTSGLADPRMADVIARSQAHVVIT
                     HSVGPPRAEKPAAHYDDVVGEVRSFLVERMARAEAAGVPHERLIIDPGHDLDKNTLHT
                     LEITRRLDELASIDVPLLVAVSNKDFIGESIDRPQGERLPGSLAAMTACILKGARIVR
                     MHDIPQTVDAVRMTEAILGWRQPVRLEHNTHPTHNV"
     gene            2253..>2809
                     /locus_tag="VN041_16640"
     CDS             2253..>2809
                     /locus_tag="VN041_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU30711.1"
                     /translation="MSDTPSIDRIWPDPRSGLDDAALLALTAFPADRTWLRVNFISSI
                     DGAATRHGLSGGLGDDADHRLFALLRRSADVVLVGAGTLRDEGYGGLRVDDASVAWRT
                     DAGLPPHPVLATISRSLSLDPASPLFADAPERPIVYTVASAPDDRRDALAGVADVVTV
                     GETDADLVRVREDLAARGLHRIHSEG"
BASE COUNT          438 a          999 c          978 g          394 t
ORIGIN      
        1 cgacgcacac ctgcatgacc ctgcgcggcg cccgcgccac cggctcgaag acgtcgacct
       61 cggcactgag gggcctgctg cgggacgacc cgcgctcccg ctccgagttc ctcgccctcg
      121 ctgcggaccg cgcgcgctga gcgcccgcat ccgcaacagt tcagcatcat cgggaaggaa
      181 gaaagatgac catcgcgatc gtcggcggag gcctcgcggc cgccaccgca gcaacggaac
      241 tgcgtgagaa gggctacgaa ggcgacgtcg tgatctactc cgccgaggat cacctgccct
      301 atgagcgccc gccgctgtcg aaggaggtgc tgctgggcag caaacaggtc gaagaggccc
      361 aggtgcacga cagcgactgg tacgccgaca acgacgtgca gctcgagcgc ggcgtgaagg
      421 tcgtctcgat cgacccggcc gcgcacacgc tgcgcgccga accggtcgcg ggcggcgaga
      481 gcaccgagca ctcctatgag aagctgctgc tcgccaccgg cgcgtcgccc cggcacttcc
      541 ccgcggccga caaggtctcc gatccggtgt acctgcgcac cgtcgaggac aacgcccgcc
      601 tgctcgaggc cctcaagccc ggtgcgaagg tgctcatcgt cggcgccggc tggatcggcc
      661 tggaggtggc gtccgccgca cgccagaagg gcgccgaggt gacgatctac gagttcgccg
      721 acctgccgct ggtcgccgtc ctcggcccgg aggtcgcgca gctgttcgcc gacctgcacc
      781 gcgctcacgg cgtggatctg cggctgagca ctcccgtgga tgaggccgcc atggcggcgg
      841 ccgacctcgt cgtggtcggc atcggggccg tccccaacac cgagctcgcc gagcaggcgg
      901 gcctcgacgt cgatcacggc gtgctggtgg acgagacgct gcgcacctcc gacccggaca
      961 tctacgccat cggtgacatc gcctcgcagc agcatccggt gctcgggcga cgaatccgcg
     1021 tggagcactg ggacacggcg atcgagcagg gcaaggtcgc cgcgcacaac ctggccggag
     1081 cctcggagca gtacgcccac ctgccgtact tcttcacaga tcagtacgat ctgggcatgg
     1141 agtacttcgg cagcgtcggc cccgaggggt acgaccgtgt cgacatcgag ggcgacaccg
     1201 acgtcgcgaa cggcggcgcg ttccgcgcct attgggtcaa ggacggcacg gtccgcgcgg
     1261 ccatgcacgc caacgactgg gactcctccg acgcggtccg ggacagcatc ggcacggctc
     1321 gatgacggca gccagcgcag catccgccgt cggagcggaa gccggcacgg ccggcgatcg
     1381 gatgctgcgc cgcatcggca tccgcgactt cgacttctcc cgtcaggtgg cggtgatggc
     1441 cgtcatcaac cggacccccg actcgttcta cgacaagggt gcgaccttcg cgctggaccg
     1501 ggccgtggag agcgcgctgc gcgccggcga ccagggggcg gactgggtcg acatcggcgg
     1561 cgttccgttc ggacgcgggc cggtggtgac gaccgccgag gagatcgacc gggtggtgcc
     1621 ggcggtcgcg gcgatcacgg ccgcccggcc ggagctggtg atctcggtgg acaccaacaa
     1681 cgccgaggtg gccgccgctg cgatcgatgc cggcgccgcg gtgatcaacg acaccagcgg
     1741 cctggctgac ccgcgcatgg ccgacgtcat cgcacgcagc caggcgcatg tcgtgatcac
     1801 gcacagcgtc ggcccgccgc gcgccgagaa gcccgccgcg cactacgacg acgtggtcgg
     1861 cgaggtgcgc tccttcctgg tcgagcggat ggcgagggcc gaggcagcgg gggtgcccca
     1921 cgagagactg atcatcgatc cgggtcatga cctcgacaag aacaccctgc acacgctcga
     1981 gatcacccgg cggttggacg agctcgcctc gatcgacgtg ccgctgctgg tcgcggtgtc
     2041 gaacaaggac ttcatcggcg agtcgatcga ccgcccgcag ggcgagcggc tgcccggatc
     2101 actggcggct atgaccgcgt gcattctgaa gggcgctcgg atcgtgcgca tgcacgacat
     2161 cccgcagacc gtcgatgccg tgcgcatgac cgaagccatc ctgggctggc gtcagccggt
     2221 gcggctcgag cacaacaccc atcccacgca caatgtctga cacgccctcc atcgatcgca
     2281 tctggccgga tccgcggtcc ggactcgacg acgcggcttt gctcgcgctc actgcgttcc
     2341 cggctgaccg cacctggctg cgggtcaact tcatctccag cattgacggt gcggcgacca
     2401 gacacggact ctccggcggc ctcggcgacg acgcggatca ccgcctgttc gccctgctgc
     2461 gccggtcggc cgacgtggtg ctggtcgggg cgggcacgct gcgcgacgag gggtacggcg
     2521 gtctgcgggt cgacgatgcg tcggtcgcgt ggcgcacgga tgccgggttg ccgccgcatc
     2581 cggtgctcgc caccatcagc cgcagcctgt ccctggaccc ggcatccccg ctgttcgcgg
     2641 acgctccgga gcgtccgatc gtctacaccg tggcatccgc ccccgacgat cggcgtgacg
     2701 cgctggcggg cgtcgccgat gtcgtgacgg tgggggagac ggatgccgat ctcgtccgcg
     2761 tgcgcgagga tctcgcggca cgcgggctgc accgcatcca ctccgaggg
//
LOCUS       DATMSX010000521         8453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_26677,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000521 DATMSX010000000
VERSION     DATMSX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26677"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN041_16645"
     CDS             complement(<1..357)
                     /locus_tag="VN041_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HWU30712.1"
                     /translation="MADRSLRGIRLGAQSLQSEEGVVFMERRATTYKCDTCGHETTLM
                     FAADAEPPQTWECRSCSAEALLQVDGASVKLEENDEKAARTHWDMLLERRTRDELEEL
                     LADRLAYVRARRGAGDD"
     gene            complement(474..1241)
                     /locus_tag="VN041_16650"
     CDS             complement(474..1241)
                     /locus_tag="VN041_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWU30713.1"
                     /translation="MTHPYFAGSRHPRVLAHRGLVPAVGEDSSLWENTAGALAAAHAA
                     GAEYIETDCRVTADGDVVLFHDDTLARLTGDRRAVSEVRTAELSQLFAPHGGLMTVGD
                     ALDAFPDTRFNIDIKTEAAAEPIGPLLAPHSHRVLVTSFSERNRRAALASVMRAGAGI
                     RPATSGASSVILALRVLSWLRLSPARVLRDIDAVQIPERHLGIRLFTPALVRAAHGCG
                     TEVHVWTVNDPDDMRRLVDAGADAIVTDRADLALKTI"
     gene            complement(1238..2164)
                     /locus_tag="VN041_16655"
     CDS             complement(1238..2164)
                     /locus_tag="VN041_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWU30714.1"
                     /translation="MDDSSFIPTAIAWILAIAVIIFVLVTIARSIRIIPQATAGIVER
                     LGRYHKTLDPGLNLLVPFIDRLRPLIDMREQVVSFPPQPVITEDNLVVSIDTVVYFQV
                     TDARAATYEIANYLGAVEQLTTTTLRNVVGGLNLEEALTSRDNINGQLRVVLDEATGK
                     WGIRVSRVELKAIDPPVSIQDSMEKQMRAERDRRAAILTAEGSKQSQILEAEGQRQAA
                     ILKAEGDKQAAVLRAQGEAEAIQNVFASIHEGRPDDKLLAYQYLQMLPKISESSSSKL
                     WIIPSELTEALKGIGSAFTPRAGGSDASQPGA"
     gene            complement(2173..2634)
                     /locus_tag="VN041_16660"
     CDS             complement(2173..2634)
                     /locus_tag="VN041_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWU30715.1"
                     /translation="MDFIAGIEQWAWIGWLVLIALFLVIEMMTLDFTFLMLSFGSVLG
                     LVSDLVGLPIWAQVIIAAAAAALFIFFLRPPLLHRLRRGEDPTKSNVAALIDLRGTAL
                     QDITGVSGQVKLSNGDTWTARSLDGTPIIQGSRVAVAEISGATAIVRPVTE"
     gene            2751..3515
                     /locus_tag="VN041_16665"
     CDS             2751..3515
                     /locus_tag="VN041_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU30716.1"
                     /translation="MTQVLPAGSLQGKVALVTGSSRGIGADTVRYFAEAGADVVINFR
                     NKAPRAEKLAAELRELGVRALVVGADLTDPSSVAEMFDAVRTEFGKLDILVLNASGGM
                     ESGMGEDYALKLNRDAQVNVLQAGEPLLGDGSRVVFVTSHQAHFIRTTPTMPEYEPVA
                     KSKRAGEDALRELIPGLAEKGIGFTVVSGDMIEGTITATLLNRLNPGAIAERRESAGK
                     LYNVSEFAAEVAQAAVDPVPADNTRLVGDTSDFTAE"
     gene            complement(3580..4173)
                     /locus_tag="VN041_16670"
     CDS             complement(3580..4173)
                     /locus_tag="VN041_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWU30717.1"
                     /translation="MSDASPEVKNFFSAVRTSLAVSGVIMLVLGIFILVSPTKTAMFF
                     AGILAVYLIIQGLVYIGTGVFTKDRGGWSRLGHIVLGLLYVVGGILAFVNLFAFTATL
                     AIFFGIMLGITWIIDGVVSLSLLGDSRSRVWTVVYAILSIVAGVILLLSPLYAVMLWW
                     LAGISLVVLGVLQIVRAVTLKKDAALIADALRSEETL"
     gene            4250..4693
                     /locus_tag="VN041_16675"
     CDS             4250..4693
                     /locus_tag="VN041_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30718.1"
                     /translation="MIDEPTRSEDRLRELVILRRVRDRIDREYAKPLDVEALARGAHM
                     SSGHLSRRFKQTFGESPYSYLMTRRIERAMALLRRGDLSVTEVCFEVGFSSLGTFSTR
                     FTELVGMPPSTYRVDHEHGPGIPSWLEKQATRPIRNREARPSPDQ"
     gene            4704..5114
                     /locus_tag="VN041_16680"
     CDS             4704..5114
                     /locus_tag="VN041_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU30719.1"
                     /translation="MNITINASFLPHTDAEASLTFYRDVLGFEVRKDVGGGRMRWITV
                     GPAEQPDTAIVLTPPAVDPGINDAERSTILELMAKGSFGGIVLATGDVDEAFAAIEAR
                     GADVVQEPIDQPYGIRDCAFRDPAGNMIRLQQIA"
     gene            5138..7486
                     /locus_tag="VN041_16685"
     CDS             5138..7486
                     /locus_tag="VN041_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWU30720.1"
                     /translation="MVHAADAHDSIRVEGARENNLKDVSVVLPKRRLTVFTGVSGSGK
                     SSLVFDTIAAESRRMIDETYSAFVQGFMPSVPRPDVDVLEGLTTAIIVDQERLGANPR
                     STVGTVTDANAMLRILFSKLGTPYIGGPTAFSFNIPTQKASGVMTGPGGEKKIVKDAI
                     YLGGMCPRCEGRGAVSDIDLAQVVDESKSLDDGAIMVPGYTADGWMVKQFAGSGFYPA
                     DKPIASFTEKQRQLFLYGEVTKVKINGINITYEGLIPKITKSMLSKDLDALQPHIRRF
                     VEQVATFATCPECDGTRLTEGARSSKIAGVSIADACRMQVTDLAAWVRGLDLDEAGPL
                     LTALSANLDSFVALGLGYLSLDRPSGTLSGGEAQRIKLLRHLGSSLTDVTYVFDEPTI
                     GLHPHDIQRMNNLLRELRDKGNTVLVVEHKPETIEIADHVVDLGPGAGAGGGEICFEG
                     TVDGLRASGTLTGRHLDDRASLKPSVRSATGAIEVRGASANNLQDVDVDIPTGVLTVI
                     TGVAGSGKSSLIHGSVSPREGVVAIDQGAIRGSRRSNPATYTGMLEPIRKAFAKANGV
                     KPALFSANSEGACPTCKGAGVIVTELGFLDTIETPCEDCGGKRFQATVLEYKLGGLDI
                     TEVLELPVAKAREFFTAADTKIPAVASVLGRLEDVGLGYLTLGQPLSTLSGGERQRVK
                     LAIQMGEKGDVYVLDEPTTGLHLADVQQILGLLDRLVDAGKTVIVIEHHQAVMAHADW
                     IIDIGPGAGHDGGRVVFEGTPSALVEERSTLTGEHLAAYVGA"
     gene            complement(7527..>8453)
                     /gene="aspS"
                     /locus_tag="VN041_16690"
     CDS             complement(7527..>8453)
                     /gene="aspS"
                     /locus_tag="VN041_16690"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HWU30721.1"
                     /translation="VTWRHATRRATWELAAASLRGFRETLDAAGFREVHSPKLVESAT
                     ESGADVFEVDYFGRPAFLAQSPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYR
                     SLDVEFGFIEDHRDVLACLRTVLAGMVSAITSYAPEAVDVLSPAIPVVPDSIPVIHFR
                     DALSRVGAPEDEPDLAPEHERQLGRWARDEHGSDFLAVEGYPMAKRPFYTHPQPSDPR
                     WSNSFDLLFRGLELVTGGQRLHRHSDYVSALRARGEDPGDYASYLPAFAHGIPPHGGF
                     AIGLERWVARLIEAGNVRETALFPRDMHRLVP"
BASE COUNT         1437 a         2782 c         2942 g         1292 t
ORIGIN      
        1 gtcgtcgccg gcgccgcggc gggcgcgcac gtaggcgagg cggtcggcga ggagctcttc
       61 gagctcatcg cgggtgcgac gctccaggag catgtcccag tgcgtgcgcg cggccttctc
      121 gtcgttctcc tcaagcttca cggatgcgcc gtccacctgc agcagcgcct cagcactgca
      181 gctgcggcac tcccaggtct gcggcggctc ggcgtcggcc gcgaacatca gggtcgtctc
      241 gtggccgcac gtgtcgcact tgtaagtggt ggcgcgccgc tccatgaaca cgacgccctc
      301 ttcgctctga aggctctggg cacccagtcg gatgcctcga aggctgcgat ctgccattgt
      361 gtggtcctct cgtcgctgtc aggtataacg gtcgcgcctg tgcgcttcat ccacagcggc
      421 gagatcccgg tcgaactcac aggggaatcc cagcgccgac cggcgcgtga cggtcagatc
      481 gtcttgagcg cgagatccgc gcgatccgtg acgatcgcgt ccgctccggc gtcgaccagg
      541 cgcctcatgt cgtcggggtc gttgaccgtc cacacgtgca cctcggtgcc gcatccgtgg
      601 gccgctcgca cgagggccgg ggtgaacagc cgaatgccca gatggcgctc ggggatctgc
      661 accgcgtcga tgtcgcgcag cacccgcgcg ggggagaggc gcagccacga cagcaccctc
      721 agcgcgagga tcaccgagct cgcgccggag gttgcgggcc ggatgccggc gccggcgcgc
      781 atcacggatg ccagcgcggc acgccgattg cgctcggaga agctcgtgac gagcacgcgg
      841 tgagaatgag gcgcgagcag cggaccgatc ggctcggcag cggcctcggt cttgatgtcg
      901 atgttgaacc gggtgtcggg aaaggcatcc agcgcatctc cgaccgtcat cagcccgccg
      961 tggggcgcga acagctggct cagctcggcc gtgcgcacct ccgacaccgc gcgccggtct
     1021 cccgtcaacc gcgcgagcgt gtcgtcatgg aacaggacca cgtcgccgtc cgccgtaacc
     1081 cggcagtcgg tctcgatgta ttcggcaccg gccgcatggg cagccgccaa ggcgccagcg
     1141 gtgttctccc acagtgagga gtcctcgccg accgcgggga ccaatccgcg gtgggcgagg
     1201 actcggggat gccgggaacc ggcgaagtac gggtgcgtca cgcgccgggc tgggaggcgt
     1261 ccgacccacc ggcgcggggc gtgaacgcac tgccgatgcc cttgagcgcc tcggtgagct
     1321 cgctggggat gatccacagt ttgctcgacg agctctcgct gatcttgggc agcatctgca
     1381 ggtactgata cgcgagcagc ttgtcgtcag ggcgaccctc gtggatggac gcgaagacgt
     1441 tctggatcgc ctccgcctca ccctgagcgc gcagcaccgc agcctgcttg tctccctctg
     1501 ccttgaggat cgcggcctgc cgctgaccct cggcctcgag gatctgcgac tgcttggagc
     1561 cctcagcggt caggatggcg gcgcgacggt cgcgctcggc gcgcatctgc ttctccatcg
     1621 agtcctggat ggacacgggc gggtcgatgg ccttcagctc gacgcgcgag actcggatgc
     1681 cccacttgcc ggtcgcctcg tcgagcacga cgcgcagctg cccgttgatg ttgtcacggc
     1741 tcgtcagcgc ctcctcgagg ttcagaccgc cgacgacgtt gcggagcgtg gtcgtggtga
     1801 gctgctcgac cgcgccgagg tagttcgcga tctcatacgt cgcggccctg gcatccgtca
     1861 cctggaagta gaccacggtg tcgatcgaga cgacgaggtt gtcctccgtg atcaccggct
     1921 gcggcgggaa cgagacgacc tgctcgcgca tgtcgatcag cgggcgcagc cggtcgatga
     1981 acggcaccag caggttcagg ccggggtcga gcgtcttgtg gtagcgaccc aggcgctcga
     2041 cgatgccggc cgtcgcctgg gggatgatcc ggatggatcg ggcgatcgtg accagcacga
     2101 agatgatgac ggcgatcgcc agtatccagg cgatcgcggt gggaatgaac gaggagtcgt
     2161 ccatcagtgc tcctattcgg tgacggggcg gacgatggcg gtggcgccgc tgatctccgc
     2221 gacagccacg cggctgccct ggatgatcgg agtgccgtcg agactgcgtg cggtccaggt
     2281 gtcgccgttg ctgagcttga cctgcccgga gacaccggtg atgtcctgca gcgcggtccc
     2341 gcgaagatcg atgagtgcgg cgacgttcga cttggtggga tcctcgccgc ggcgcagccg
     2401 gtgcagcagc ggcgggcgca agaagaagat gaacagcgcc gcggcggccg cagcgatgat
     2461 gacctgcgcc cagatcggca ggccgacgag atcggagacg agccccagca cgctgccgaa
     2521 ggacagcatc aggaacgtga agtccagggt catcatctcg atgaccagga acagggcgat
     2581 cagcaccagc cagccgatcc aggcccactg ctcgatacct gcgatgaagt ccatgcgccc
     2641 ctcctgcggt caacctatca cgcgggtgat cccgcggtgc ggcaacgccg cgttgtaggg
     2701 ttgggaggtc gccgaaccgg cgatatcccg cgcatcacga ggagtcagga atgacccagg
     2761 ttctgcccgc cggatccctt cagggcaagg tggcgctcgt caccggctct tcccggggca
     2821 tcggcgccga caccgtccgc tacttcgccg aggccggtgc ggacgtcgtc atcaacttcc
     2881 gcaacaaggc gccgcgtgcc gagaagctcg ccgcggagct ccgggagctg ggagtgcggg
     2941 cgctggtcgt gggcgccgat ctcaccgatc cctcctccgt cgcggagatg ttcgacgcgg
     3001 tgcggacgga gttcggcaag ctcgacatcc tcgtgctcaa cgcctcgggc ggcatggagt
     3061 ccggcatggg tgaggactac gctctcaagc tcaaccgcga cgcgcaggtc aacgtgctgc
     3121 aggccggcga gcccctgctg ggcgacggct cgcgcgtcgt gttcgtgacc agccatcagg
     3181 cgcacttcat ccgcaccacg ccgacgatgc ccgagtacga gccggtcgcg aagtccaagc
     3241 gcgcgggtga ggacgccctg cgcgagctga tccccggtct cgcggagaag ggcatcggct
     3301 tcacggtcgt ctccggcgac atgatcgagg gcacgatcac cgccacgctt ctgaaccgcc
     3361 tcaaccccgg cgcgatcgcg gagcggcgcg agtccgcggg caagctctac aacgtgtccg
     3421 agttcgccgc ggaggtggcc caggccgctg tcgaccccgt ccccgcagac aacacccgtc
     3481 tggtcggaga cacctcggac ttcaccgccg agtgatccgc tgaacgagga aggccccgtc
     3541 cgatcggacg gggccttcct cgttcgctga cgagacgcgt cagagggtct cctccgaacg
     3601 aagggcgtcg gcgatgagcg cggcatcctt cttgagagtg acggcacgca cgatctgcag
     3661 gacaccgagc acgacgaggg agatgccggc gagccaccac agcatgaccg cgtacagcgg
     3721 cgagagcagc aggatgacgc cggcgacgat gctgaggatc gcgtagacca cggtccagac
     3781 cctcgagcgg gaatcgccca gcagcgacag cgagacgacg ccgtcgatga tccaggtgat
     3841 gccgagcatg atgccgaaga agatcgcgag cgtcgccgtg aacgcgaaga ggttgacgaa
     3901 cgcgaggatg ccgccgacga cgtagagcag gccgaggacg atgtggccga ggcgcgacca
     3961 gccgcccctg tccttcgtga agacgcccgt gccgatgtac accagaccct ggatgatcag
     4021 gtacacggcg aggatgcccg cgaagaacat cgcggtcttg gtcggggaga cgaggatgaa
     4081 gatgccgagc accagcatga tcacgccgga gacggccagc gaggtgcgca ctgcggagaa
     4141 gaagttcttc acttcggggg aagcgtcaga cacgatgatg tcctttcctg tcatctcctg
     4201 agagtgggag atggcgccca gcgtactggc tgcatgcgac gatcggtgtg tgattgatga
     4261 accgacacgc tccgaggaca ggctgcgaga gctcgtcatc ctgcgccggg tgcgggatcg
     4321 catcgaccgc gagtacgcga agccgctcga cgtggaggcg ctggcgcgcg gggcgcacat
     4381 gtcgtcgggc cacctcagcc gccggttcaa gcagacgttc ggcgagtccc cgtactccta
     4441 tctgatgaca cggcggatcg agcgggccat ggcgcttctg cgccgcgggg atctgtccgt
     4501 caccgaggtg tgcttcgagg tcggcttctc ctcgctcggc acgttcagca cgcgtttcac
     4561 cgagctggtg gggatgccac cgagcaccta tcgcgtcgat cacgagcatg gtccgggcat
     4621 cccgtcgtgg ctggagaagc aggcgaccag accgatcagg aatcgagaag cgcggccatc
     4681 gccggatcag tagcgtcggt gacatgaaca tcaccatcaa cgcgagcttc ctcccgcaca
     4741 cggatgccga ggcctcgctg accttctatc gcgacgtgct cggattcgag gtgcgcaagg
     4801 acgtcggagg gggccggatg cggtggatca ccgtcggtcc ggccgagcag cccgacacgg
     4861 cgatcgtgct gactccgccc gccgtcgacc ctggcatcaa cgacgccgag cgcagcacga
     4921 tcctcgagct catggccaag gggagcttcg gcggcatcgt cctcgcgacc ggcgacgtcg
     4981 acgaggcgtt cgccgccatc gaggcgaggg gcgccgacgt cgtgcaggag ccgatcgacc
     5041 agccgtacgg catccgcgac tgcgccttcc gggacccggc aggcaatatg atccgactgc
     5101 agcagatcgc ctgaccgcca cccagaagga gacgccgatg gtccacgccg ccgacgccca
     5161 tgacagcatc cgagtcgaag gggcccgcga gaacaatctg aaggacgtca gcgtggtgct
     5221 ccccaagcgt cgtctgaccg tgttcaccgg ggtgtccggg tcgggcaaga gctcgctggt
     5281 cttcgacacg atcgctgcgg agtcgcgacg gatgatcgac gagacctaca gcgccttcgt
     5341 gcaggggttc atgccttcgg tgccgcgtcc cgacgtggat gtgctggagg ggctgacgac
     5401 cgcgatcatc gtcgaccagg aacggctcgg agccaacccg aggtcgaccg tcggcaccgt
     5461 gaccgacgcc aatgcgatgc tgcgcatcct gttctccaag ctcggcaccc cctacatcgg
     5521 cgggcccacg gcgttctcat tcaacatccc gacccagaag gccagcggag tcatgaccgg
     5581 gccgggcggc gagaagaaga tcgtgaagga cgcgatctat ctcggcggca tgtgcccgcg
     5641 gtgcgagggc aggggagccg tatcggacat cgatctcgcg caggtggtcg acgagtcgaa
     5701 gtcattggac gacggcgcga tcatggttcc cggctacacg gccgacgggt ggatggtcaa
     5761 gcagttcgcg ggttcgggct tctacccggc cgacaagccc atcgcgtcgt tcaccgagaa
     5821 gcagcgtcag ctgttcctct acggcgaggt caccaaggtc aagatcaacg ggatcaacat
     5881 cacctacgag gggctcatcc ccaagatcac gaagtccatg ctgtccaagg acctcgatgc
     5941 gctgcagccg cacatccggc gcttcgtcga gcaggtcgcg accttcgcca cctgtccgga
     6001 gtgcgacggc acccgcctca cagagggagc gcggtcgtcg aagatcgccg gggtgagcat
     6061 cgcggacgcc tgccggatgc aggtgaccga cctcgccgcg tgggtgcgcg gactggatct
     6121 ggacgaggcg ggcccgctgc tgaccgcgct gagcgcaaac ctggactcct tcgtcgccct
     6181 ggggctcgga tacctgagcc tggatcgtcc gtccgggacg ctgagtgggg gagaggccca
     6241 gcgcatcaag ctgctgcgcc acctcggatc gtccctcacc gacgtcacct acgtgttcga
     6301 cgagccgacg atcgggctgc atccgcacga catccagcgg atgaacaacc tgcttcgcga
     6361 actccgcgac aaggggaaca cggtgctggt cgtcgagcac aagccggaga ccatcgagat
     6421 cgccgaccac gtcgtcgatc tcggccccgg agcgggtgcg ggcggcggcg agatctgctt
     6481 cgagggaacg gtggacgggt tgcgggcgag cggcacactg acgggtcgtc acctcgacga
     6541 ccgggcctcg ctgaagccct ccgtgcgcag cgcgacgggc gcgatcgagg tgcgcggagc
     6601 cagcgcgaac aacctgcagg acgtggacgt cgacatcccc accggggtgc tgacggtcat
     6661 cacgggtgtc gccggttcgg gcaagagctc gctcatccac ggctcggtct cgccgcggga
     6721 gggagtcgtc gcgatcgacc agggtgcgat caggggctcg cgacgcagca atccggcgac
     6781 gtacaccggc atgctggagc ccatccgcaa ggccttcgcc aaggcgaacg gggtcaagcc
     6841 cgcgctgttc agcgcgaact ccgagggcgc ctgcccgacc tgcaagggcg ccggcgtgat
     6901 cgtcaccgag ctcgggttct tggacacgat cgagaccccc tgcgaggact gcggcggcaa
     6961 gcgcttccag gccacggtgc tggagtacaa gctcggcggc ctggacatca ccgaggtgct
     7021 ggagctgccg gtggcgaagg cgcgtgagtt cttcacagcc gccgatacga agatcccggc
     7081 ggtggcgagc gttctcgggc ggctggaaga tgtcggcctg ggctatctca ccctcggtca
     7141 gccgctgtcg acgttgtcgg gcggtgaacg tcagcgggtc aagctcgcga tccagatggg
     7201 ggagaagggc gacgtctacg tcctcgacga gcccacgacg gggctgcacc tcgctgacgt
     7261 gcagcagatc ctgggcctgc tggaccggct ggtggatgcg gggaagaccg tcatcgtcat
     7321 cgagcaccac caggctgtca tggctcacgc cgactggatc atcgacatcg gtcccggtgc
     7381 ggggcacgac ggcggacgcg tcgtcttcga gggaaccccg tctgccctcg tcgaggagcg
     7441 gtcgacgctc acgggggagc acctcgcggc atacgtgggc gcttgagcga gatggggacg
     7501 gatgcgggca tccgcccccc tcctggtcac ggcacgagcc ggtgcatgtc cctggggaag
     7561 agcgccgtct cccggacgtt gcccgcttcg atgagccgcg cgacccatcg ctccagtccg
     7621 atcgcgaagc cgccgtgcgg cgggattccg tgcgcgaacg ccggcaggta cgacgcatag
     7681 tcgcccgggt cctcgccccg cgcccggagg gcggagacgt agtcggagtg ccggtgcagg
     7741 cgctggccgc ctgtcaccag ctcgagaccg cggaaaagca ggtcgaagct gttgctccag
     7801 cgcgggtcgg acggctgcgg gtgcgtgtag aacggacgct tcgccatcgg ataaccctcg
     7861 accgcgagga agtcgctgcc gtgctcgtcg cgcgcccagc gccccagctg ccgctcgtgc
     7921 tccggcgcga ggtcgggttc gtcctccggt gcgcctacgc gactcagtgc gtcgcggaag
     7981 tggatgaccg ggatgctgtc gggcacgacc gggatcgcgg gcgacagcac atcgaccgcc
     8041 tccggcgcgt acgaggtgat cgctgagacc atgccggcga gcactgtgcg caggcaggcg
     8101 agcacgtcgc ggtgatcttc gatgaacccg aactcgacat cgagggagcg gtactccgcc
     8161 agatgccgca cggtgtcgtg aggttcggcc cggaagaccg gaccgacctc gtagacgcgc
     8221 tcgaacacgc cgacgagctg ctgtttgtag aactggggac tctgcgccag aaaggccggg
     8281 cgaccgaagt agtccacctc gaacacgtcc gcgccggact cggtcgccga ctccacgagc
     8341 ttgggggagt gcacctcccg gaacccggcg gcgtccagcg tctcccggaa gccgcgcagg
     8401 ctcgcggccg cgagctccca ggtcgcgcgt cgggtggcat ggcgccacgt cac
//
LOCUS       DATMSX010000522         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_55529,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000522 DATMSX010000000
VERSION     DATMSX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55529"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2276
                     /locus_tag="VN041_16695"
     CDS             <1..2276
                     /locus_tag="VN041_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWU30722.1"
                     /translation="RMSQAPHAAVDSVEHVPAALALRQRPGVPGVRSTFGTSTELLNV
                     LRLMFSRLASHVCPNGHHVPPTIDVAAETPFACPVCGERVHAPGAEALAFNSEGACPT
                     CQGTGVVRTVDDATLVPDPSKTLDGGAVAPWQMFGFNVQPDIAREFGVRTDVPWSALT
                     DAEREIVLNGPEEKKHITVTSRKGVHELDFTFRNARLTVTEELARAADEKRLARVSRF
                     LTEGMCPDCGGTRLSPQARAPRIGEMGLAEVTAMTLDDVIAWAPGVTATLPREMQPLA
                     SDLIATLLAMARRLVELGLGYLSLDRAGATLSTGERQRAQLARAVRNRTTGVLYVLDE
                     PSIGLHPANVEGLLGVMRDLLADGNSVVFVDHDVQILAEADWLVEIGPGAGTEGGRVI
                     AQGAPRALGEASRLRGFLTGDEPVVVRERSAPDAVFEHGAIRMSTDAVHTVHPLEVAV
                     PVGRLTAVTGVSGSGKTTLVLESLVPALNASAGGRMPAHVRSLDVPSVLRVHVVDATP
                     IGLNVRSTVATYSGVMDELRKAYAATDAAKQAGLTASDFSYNTGSLSCPRCEGTGEIS
                     LDVQFLPDVDIVCPECEGSRFAPAASDYVRDGITLPALLRCTIAEALARTADLPRVHR
                     RLQAFSELGLGYLTLGEATPALSGGEAQRLKLVNQMHRDQSDAVFVLDEPSVGLHPLD
                     VRTLLSVFDRLVSRGATVIVIEHDLDVIANADHVIDMGPGGGAEGGRIVAVGAPDRIA
                     ADPASLTGVHLAHHLRQ"
     gene            2286..2801
                     /locus_tag="VN041_16700"
     CDS             2286..2801
                     /locus_tag="VN041_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30723.1"
                     /translation="MRFEALLRRLDRIAGGRHADLEVSAEMREHPKTRRAFTWITWLL
                     AVEVLLGIAAVVVAVVIVVNGGPVPFAVWMRSVIVLGMTVTLFYFSWRASLGWYWAYQ
                     RLRLFAQIFPIITLVMAAIPNLYPAWMVTEQIVFSLIMIGVADILTGDHMRTAFRRPE
                     RPQRHSPMKPR"
     gene            complement(2771..3664)
                     /locus_tag="VN041_16705"
     CDS             complement(2771..3664)
                     /locus_tag="VN041_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30724.1"
                     /translation="MTLSIDQFRAALLDSHQRAGLGIALTDTDAQLVVADPVSSQTWY
                     GYWISIGAPHETPAAAQQPHPDTEPVLPFTPPGQSFAAPTAMSGVDSQSGTLPFPAAH
                     PTVSVSPTAVGMTAVIESGPSGVFDAAPPRRRKNVGLWVTLALVGVLILAVAGTTVYA
                     FVTARHWDKVDVPAQAATTHSEQYETGRYDVTMDTVNPCAVGQDWTECTDAMARQYEL
                     ACAGVELTESAVPTCEQYSSAIAEMKRQDGDGYYVESLGDYGHLSRTPEIGTREVSNN
                     DARPAITHRAACYLGFIGECR"
     gene            complement(3678..4172)
                     /locus_tag="VN041_16710"
     CDS             complement(3678..4172)
                     /locus_tag="VN041_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWU30725.1"
                     /translation="MANTHGSTATVDALDLRILSALSAQPDAPVKQLAAMLGIPASTC
                     AFRVRSLRARGVIAGLDLVIDPAALGATVQAMIRVRLANHSKSGVDALFDALVSTPGV
                     LQVFHIAGADDFLVHVAVTDATALRDIVLEHITVHDVVRATETQLVFERRQGAGVLTR
                     RAPS"
     gene            4286..>5057
                     /locus_tag="VN041_16715"
     CDS             4286..>5057
                     /locus_tag="VN041_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWU30726.1"
                     /translation="MSDLPLHPETVAVHAGRSDFAAMGVHAAPIDLSSTNPLPDILHG
                     GDSYESMANGGHPLPDGGNVYARLWNPTVARFEEAFAELEHAEAAVAFSSGMAATTAA
                     ILSHTTAAGKHHVVAVRPLYGGTDHLLDSGLLGAEVTFCHEAEVAASIRPDTGLIVLE
                     TPANPTLDLVDIASVVAQAQGVPVMVDNTFATPLLQNPLDLGAAMSLHSATKYIGGHG
                     DVIAGVIACSEETAQALRRVRAITGALLHPLGAYLLHRG"
BASE COUNT          735 a         1725 c         1793 g          804 t
ORIGIN      
        1 ggcggatgtc gcaggcgccg cacgccgccg tcgactccgt cgagcacgtc ccggcggcgc
       61 tcgccctcag gcagcgtccg ggcgtgcccg gtgtgcgctc cacattcggc acctccacgg
      121 agctgctgaa cgtgctgagg ctgatgttct cgcgcctggc atcgcatgtc tgcccgaacg
      181 ggcatcacgt gccgccgacg atcgacgtag ccgccgagac gccgttcgcc tgcccggtct
      241 gcggtgagcg cgtgcacgcc ccgggcgccg aggcgctggc gttcaactcg gagggagcct
      301 gcccgacgtg ccagggcacg ggggtggtgc gcacggtcga cgacgcgacg ctggtgcccg
      361 acccgtcgaa gacgctcgac ggcggtgcgg tggcgccctg gcagatgttc ggcttcaacg
      421 tgcagcccga tatcgcccgc gagttcggcg tgcgcaccga cgtgccgtgg agcgcactca
      481 cggatgccga gcgcgagatc gtgctgaacg gtcccgagga gaagaagcac atcacggtga
      541 ccagccgcaa gggcgtgcac gagctggact tcacgttccg caacgcccgg ctcaccgtga
      601 cggaggaact cgcccgtgcg gcggacgaga agcgcctggc cagagtgagc cggttcctca
      661 ccgagggcat gtgcccggac tgcggcggga cgaggctgag tccgcaggcg cgagcgccgc
      721 ggatcggcga gatggggctg gcagaggtca ccgcgatgac cctggatgac gtgatcgcct
      781 gggctccggg agtgacggcg acgctgccac gcgagatgca gccgctggca tccgatctga
      841 tcgccaccct gctcgccatg gcgcggcggc tggtcgagct cggactcggc tacctgtccc
      901 tggaccgcgc cggcgccacc ctgtcgacgg gcgagaggca gcgcgcccag ctggcacgcg
      961 ccgtgcgcaa ccgcaccacc ggcgtgctct atgtgctgga tgagccttcg atcggtctgc
     1021 atcccgccaa tgtcgagggt ctgctcggcg tcatgcgcga cctgctggcg gacggcaact
     1081 cggtcgtctt cgtggaccac gacgtgcaga tcctcgccga ggccgactgg ctggtcgaga
     1141 tcggtccggg cgccggtacc gagggcggtc gcgtgatcgc gcagggcgct ccgcgcgccc
     1201 tgggcgaggc atcccgcctg cgtggtttcc tcacggggga cgagccggtg gtcgtgcggg
     1261 agcggagcgc gcccgatgcg gtgttcgagc acggggcgat caggatgtcg acggatgccg
     1321 tgcacaccgt gcatccgctc gaggtggccg taccggtcgg ccggctcacg gccgtcaccg
     1381 gcgtctcggg ctccggtaag acgacgctcg tgctggagtc gctggtgccg gcgctcaacg
     1441 catccgcggg cgggcggatg cccgcgcacg ttcgctccct cgatgtgccg tcggtcctgc
     1501 gggtgcacgt cgtggatgcg acgccgatcg ggctgaacgt gcgctcgacc gtggcgacct
     1561 actccggcgt gatggatgag ctgcgcaagg cctacgcggc gacggatgcc gcgaagcagg
     1621 cggggctcac ggcatccgac ttctcctaca acacgggctc gctgtcgtgt ccgcggtgcg
     1681 agggaaccgg cgagatctcc ctcgatgtgc agttcctgcc agacgtcgac atcgtctgcc
     1741 cggagtgcga gggcagcagg ttcgcgccgg ctgcctcgga ctatgtccgc gacgggatca
     1801 cgctgcccgc tctgctgcgc tgcacgatcg ccgaggcgct cgcacgcacc gccgacctgc
     1861 cgcgcgtgca cagacggctg caggcgttct ccgaactcgg cctcgggtac ctcacgctcg
     1921 gcgaggcgac gcccgcgctc tccggtggcg aggcgcagcg cctcaagctc gtgaatcaga
     1981 tgcaccgcga ccagtcggac gccgtgttcg tgctcgacga gcccagcgtc ggactgcatc
     2041 ctctggacgt gcgcacgctg ctgtccgtct tcgaccggct cgtctctcgc ggcgcgacgg
     2101 tgatcgtgat cgagcacgat ctggacgtga tcgcgaatgc cgatcacgtg atcgacatgg
     2161 gaccgggcgg cggcgcggag ggcgggcgga tcgtcgccgt cggagcgccg gaccgcattg
     2221 cggcggatcc tgcctcgctc accggcgtgc atctggctca tcacctgcgg cagtagcatc
     2281 gtccggtgcg cttcgaagcc cttctccgtc gtctggaccg gatcgccggc ggacgccacg
     2341 ccgatctcga ggtgtccgca gagatgcgag agcacccgaa gacgcgtcgc gcgttcacct
     2401 ggatcacctg gctgctggcc gtggaggtgc tcctcggcat cgcggccgtg gtcgtcgcgg
     2461 tcgtcatcgt cgtgaacggc ggaccggtgc cgttcgcggt gtggatgcgc agcgtgatcg
     2521 tgctcggcat gaccgtgacg ctgttctact tctcctggcg ggcatcgctc ggctggtact
     2581 gggcgtatca gcgtctgcgg ctcttcgcgc agatcttccc gatcatcacg ctcgtgatgg
     2641 cggcgatccc gaatctctac ccggcctgga tggtgaccga gcagatcgtg ttcagcctga
     2701 tcatgatcgg cgtcgccgac atcctgaccg gcgaccacat gcgcaccgcg ttccgacggc
     2761 cggagcggcc tcagcggcac tccccgatga aaccgaggta gcaggcggcg cggtgcgtga
     2821 tcgccggacg agcatcgttg ttcgacacct cccgggtacc gatctcgggc gtccgtgaca
     2881 ggtgcccgta gtccccgagc gactcgacgt agtagccgtc gccgtcctgg cgcttcatct
     2941 cggcgatcgc ggagctgtac tgctcgcatg tcggcaccgc ggactcggtg agctccactc
     3001 ccgcgcaggc gagttcgtac tgccgcgcca tggcatccgt gcattcggtc cagtcctgcc
     3061 cgaccgcaca ggggttgacg gtgtccatgg tgacgtcgta ccgcccggtc tcgtactgct
     3121 cggaatgcgt cgtcgccgcc tgcgcgggca catccacctt gtcccagtgg cgagcggtga
     3181 cgaacgcgta gaccgtggtg cctgcgacag ccaggatcag cacgccgacg agcgccagag
     3241 tgacccagag gccgacgttc ttccggcgcc gcggaggcgc ggcatcgaac accccggacg
     3301 gtcccgactc gatcacggcg gtcatgccga ccgcggtggg tgagaccgac acggtcgggt
     3361 gggcggcggg aaacggcaga gtgccggact gtgagtcgac gccagacatg gctgtcggcg
     3421 cggcgaagct ctgtcccgga ggggtgaacg gcaggaccgg ctcggtgtct gggtgcggct
     3481 gctgggcagc cgccggtgtc tcgtgcggtg cgccgatcga gatccagtac ccgtaccagg
     3541 tctgtgagga caccggatcg gcgacgacca gctgcgcgtc cgtgtcggtg agggctatcc
     3601 cgagtccggc gcgctggtgg gagtcgagca gtgcggcgcg gaactggtcg atggacagag
     3661 tcatgaaggt gatgatgtca tgacggggca cgcctggtca gcacgcccgc gccctgacgg
     3721 cgctcgaaca cgagttgcgt ctccgtggcg cgcacgacgt cgtgaacggt gatgtgctcc
     3781 agcacgatgt cgcgcagcgc cgtggcatcc gtcaccgcga cgtgcaccag aaaatcgtcc
     3841 gcgcccgcga tgtggaacac ctgcagcacg cccggagtgg acaccagcgc gtcgaacagg
     3901 gcgtccacgc cgctcttgct gtggttggcc agccgcacgc ggatcatcgc ctgaaccgtc
     3961 gcccccagcg ccgcggggtc gatcaccaga tccagcccgg cgatcacgcc gcgagcgcga
     4021 agcgagcgca cgcggaaggc gcaggtggat gccgggatgc cgagcatcgc ggcgagctgc
     4081 ttcaccgggg catccggctg agcggacaga gccgacagga tgcggagatc gagtgcgtcg
     4141 acagtggccg tcgatccatg cgtgttggcc aacgcgtcct ccgtccgggc agtcctggtt
     4201 gctctgcgag caagattatc gtcgtcagag cgtcgatgcg acaaggatgc tggattcgcg
     4261 tcgctctgcg acgcatcctg gtggcatgtc ggatctgccg ctgcatcccg agaccgtcgc
     4321 cgtccacgcc gggcgctccg atttcgccgc gatgggggtg cacgccgcac ccatcgacct
     4381 gtcgtcgacc aacccgctgc ccgacatcct gcacggtggc gactcctatg agtcgatggc
     4441 gaacggcggg cacccgctgc cggatggcgg caacgtgtac gcccggctct ggaacccgac
     4501 ggtggcgcgc ttcgaggagg cgttcgcgga gctcgagcac gccgaggccg cggtggcgtt
     4561 cagctcgggt atggcggcca cgaccgccgc gatcctgtcg cacacgacgg cagcaggcaa
     4621 gcaccatgtc gttgcggtcc gtccgctcta cggcggtacc gatcacctgc tggactcggg
     4681 cctcctcggc gccgaggtca cgttctgcca tgaggccgag gtcgctgcca gcatccggcc
     4741 cgacaccggg ctcatcgtgc tggagacgcc ggccaacccg actctcgacc tcgtcgacat
     4801 cgcgtccgtc gtcgcgcagg cgcagggcgt gccggtgatg gtcgacaaca ccttcgccac
     4861 cccgctgctg cagaacccgc tcgacctcgg cgcggccatg tccctgcaca gcgcgacgaa
     4921 gtacatcggc ggtcacggcg acgtgatcgc cggcgtgatc gcctgctcgg aggagaccgc
     4981 gcaggcgctg cgccgggtgc gtgcgatcac gggcgcgctc ctgcacccgc tcggcgcgta
     5041 cctgctgcac agaggac
//
LOCUS       DATMSX010000523         9474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149736,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000523 DATMSX010000000
VERSION     DATMSX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149736"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VN041_16720"
     CDS             <1..1215
                     /locus_tag="VN041_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWU30727.1"
                     /translation="IGAGILGLTRPDAGMLAWLPSVLAGAVAAFALRMLLRRVDDAGP
                     EGEGQPSRRAFLTWTIGTAAVGVAMAVVGGLGRAGSTVVTTVREALKLPKPAVPAPAI
                     PASASLDVAGITPVITPNADFYRIDTALVVPSVDPSSWKLRIHGMVAQEVTLTWDELL
                     ALPLVESVATLSCVSNEVGGNLIGNAVWLGYPIRELLKRAAPDADADMVLSTSVDGFT
                     AGTPLEALTDERAALLAIGMNGQPLPLEHGFPVRMVVPGLYGYVSATKWVTDLEVTRF
                     DRARAYWTDRGWSERGPIKLESRIDVPREGRTVAAGDSVIAGVAWQPGTGVSGVEVQV
                     DDGDWQPAELATAIADDTWVQWRVPWKAKAGEHSIRCRAIAGDGTVQTDTIAPPAPDG
                     ASGWHTISVSVS"
     gene            1305..2291
                     /locus_tag="VN041_16725"
     CDS             1305..2291
                     /locus_tag="VN041_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30728.1"
                     /translation="MNTIISTRGLTKTYGRVHALDGLDLEVEAGQVHGFLGPNGAGKS
                     TTIRILLGLARKSGGTASVFGLEPWGDAVQIHRRVAYVPGDVSVWPNLSGGEAIDFLA
                     RLRGTKTKDAAYRAERERLMTAFQFDPRKKGRAYSKGNRQKVALIAAFAVPADLYILD
                     EPTSGLDPLMEVVFRDEVQRLRRSGATVLLSSHILSEVELLCDRVSIIREGKIVESGT
                     LSELRHLTRTEVSFEAASADALAGIPEAHDGTVEEGRARFTVDSDSIPSVLPVLAQRA
                     VTGLRIEPPSLEELFLRHYGDDLAALRAAEEDSSDAGAGTRASRRAAAKGTR"
     gene            2291..3904
                     /locus_tag="VN041_16730"
     CDS             2291..3904
                     /locus_tag="VN041_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU30729.1"
                     /translation="MFRSLLRQRLRRDWLQLLLWILATVLMAYSGFAGVAQSYATHKD
                     RVEVLAAVMANPVIMMFRGLPSGASEGQFLSFEILPWLTMLAALMSTFLAVRHTRGDE
                     EAGRTELLAATPAGRALPTVATIVHGLAANVVLGVLVAFALAGSKLPAAGSWLTGAVS
                     ATTGIAFLGIGLIAAQLMRTSRGANSLTVWILVGTFLINGIGNVAGTPSDDLTRISSS
                     GLVWLSPFGWAEQARPFDTDDAWPALLGLGFGLVLAVGSVALQSLRDIGEGFIPARPG
                     RVFARPALSSPHALVWRLTNGAIIGWAVGGALTGILATKLSGLAGQISGQNPAVQQIL
                     DKIGKAGNLEETVVSVFFTMMGILAACAAVQILARARQEEAHGTAEIVLAQPVGRVRW
                     LLDYLLVATIAIAIIVAATMLAAFAGLAGNEDASALAKVVTVVGLGQGLAACVFTALT
                     ALAFVLLPRGTIAIAWALVMLATVFGLFGPLFGLPEWTTRFSPFAVTPIISDGTTDLR
                     GTWWLVIIVAVVATASLSLMRRRELRPAA"
     gene            3965..4447
                     /locus_tag="VN041_16735"
     CDS             3965..4447
                     /locus_tag="VN041_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30730.1"
                     /translation="MSEDVTHAGVDAAESAAAAITAVGFPRMPARVMMALIAAPSEGY
                     TASELGDRLGVSAAAVSGAVRYLQTLHVIRKVSRPDQRLARYEIIPDTWYVMMSNNSP
                     VYNRLAAYIDEIGAAHAEDHAARLRAEEMAEFFRFMSLRMPQLVDEWETLRAERRLQS
                     "
     gene            4606..6453
                     /locus_tag="VN041_16740"
     CDS             4606..6453
                     /locus_tag="VN041_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWU30731.1"
                     /translation="MTTAGNEAGTSRREPQVLRWVFWPAAVIVLAFALFALLAPHIAE
                     ALFKTVQTTIVNAFNWYYVLIAAFFVAFCLFLGFSRFGDIKLGRDDEEPEFSLMSWFS
                     LLFAAGMGIGLVFYGVSEPLSHFTNPRPGVTGTPEQLAQAALGQTYLHWGVHAWSIYV
                     VIGLALAYAIHRRRRPVSIRWTLEPLLGKRVEGGWGHAIDVIALVGTLFGVATSLGLG
                     VLQMSAGLHSAGFGNPDETTQVVLILIISVFVLMSVLSGVTKGMKWLSNANLVLAGLL
                     VIYLLAAGPTEFLLREFVQSLGYYIQNFVGLSFNVSAFQGAAGEQWQASWTSFYWGWW
                     ISWAPFVGIFIARVSKGRTVREFVIGVILVPTLIGVLWFSVLGGSALAMELAKPGSLT
                     GPDGTVDLQGALFAMLQNVPGATVVSIGAILLLAIFFITSADSGALVMGMIATGGKPE
                     PKRWIRTFFVVITALLAISLLLSGGLTALQTAAITIALPFSIVMLLICWSTVVAFTRE
                     RRAYAKAERAQFIDRIGEYYGLEVESHDATGVLGAQPRWMRRLQRRFGLPVAPTEPIR
                     ITPGALSLESHGDASPSTLDVDDLVGGDELAGLDDPDVKQQDAGPFLKE"
     gene            6505..6816
                     /locus_tag="VN041_16745"
     CDS             6505..6816
                     /locus_tag="VN041_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30732.1"
                     /translation="MDERQSSQDQDAGQQPHTPGQDPAGSGEPHEAPQSADAPDGSEG
                     AHTGIPEDNPAHNTAPDGMPGDEEGAPLGGDERTEEQLDADTAVERDALKSLDPDDTP
                     A"
     gene            complement(6886..7761)
                     /locus_tag="VN041_16750"
     CDS             complement(6886..7761)
                     /locus_tag="VN041_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-dimethylarginine dimethylaminohydrolase"
                     /protein_id="HWU30733.1"
                     /translation="MSTPETAATGTATRKAHHRRYLMCRPEHFTVDYKINPWMDPAAP
                     TDTAKAVAQWQKLYDLYLELGHEVELIEPLAGYPDMVYTANGGFTIGGKAYVPEFYYE
                     QRQGEAPAFAEWFRTHGFDTVLPQEVNEGEGDFLLAGDVILAGTGFRSAGDSHREVHE
                     VFDREVVTLTLVDPRFYHLDTALTVLDPVVGEENGGPEHANVAYLPNAFDEASRRILE
                     ERFPDAILVADEDGAVFGLNSASDGYNVIISPRATGFEKQLRERGYNPIMVDLSELLL
                     GGGGIKCCTLELRGA"
     gene            complement(7939..>9474)
                     /locus_tag="VN041_16755"
     CDS             complement(7939..>9474)
                     /locus_tag="VN041_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU30734.1"
                     /translation="AAVIAEALWQAGIPRDVLVLADVEEGELGQQLISHPDVDRVILT
                     GSWDTAALFRSWRSDLPLLAETSGKNAMIITPTADLDLAVADLVHSAFGHAGQKCSAA
                     SLAILVGPVGRSKRFARQLVDATRSLRVAWPGDPQAEVGPVIEKPQGKLAWALTQLEG
                     EEQWLVEPRSLDDSGRLWSPGIRVGVQPGSRTHREEFFGPVLGIMHASTLSEAIRMQN
                     AVAYGLTAGLHTQDPADLALWLDEVQAGNLYVNRGITGAIVQRQPFGGWKRSSVGPGA
                     KAGGPNYLIGLGSWRSRPRGKSSSTLHLRGLDVKISGLIESAQPSLDFDSFEWLRQAA
                     LSDAVAWDREFGQVRDVSQLGVERNLFRYRPLPVTVRAASDASLAAILRVVLAGIRSG
                     ADFFVSVPDGVPAGVRAELGALEVPVAVESDEEWIERIRRPAPEATRVGRVRLIGGQD
                     AVADLHRALADAVEGDPDLAVYDNEVTSAGRLELLPFVHEQAISITAHRFGTPDPWSA
                     EVI"
BASE COUNT         1356 a         3347 c         3289 g         1482 t
ORIGIN      
        1 atcggcgccg gcatcctcgg gctgacccga ccggatgccg ggatgctggc ctggctgccg
       61 tccgtgctcg ccggcgcggt cgcggccttt gcactgcgga tgctgcttcg ccgtgtggac
      121 gatgccgggc ctgaaggtga agggcagccg tcacgtcgag cgttcctcac ctggacgatc
      181 ggcacggccg ccgtgggcgt ggcgatggct gtcgtcggcg ggctggggcg ggcgggctcc
      241 acggtcgtca cgacagtgcg cgaggccctc aagctgccga agccggcggt tcccgccccg
      301 gcgatcccgg catccgcgtc gctcgacgtc gccgggatca cccccgtgat cacgccgaac
      361 gcggacttct accgcatcga cacggcgctc gtggtgccgt cggtcgaccc gtcgagctgg
      421 aagctgcgta tccacggcat ggtcgcgcag gaggtgacgc tcacgtggga cgaattgctc
      481 gctttgcccc tggtcgagag cgtcgcgacc ctgtcctgcg tgtcgaacga ggtcggcggg
      541 aacctgatcg gcaacgcggt ctggctgggc tatccgatcc gcgagttgct gaagcgcgcg
      601 gctcccgacg cggatgccga catggtgctc tccacgtcgg tcgacggctt caccgcagga
      661 acgcccctcg aggcgctcac cgacgagcgc gccgcactgc tcgcgatcgg gatgaacggg
      721 cagccgctcc ccctcgagca cggcttcccg gtgcggatgg tcgtgccagg cctgtacggg
      781 tacgtgtccg cgacgaagtg ggtgaccgat ctcgaggtga cccggttcga cagggcgagg
      841 gcgtactgga ccgaccgcgg atggtcggag aggggcccga tcaagctgga gtcccgcatc
      901 gacgtacccc gtgaggggcg caccgtggcc gcgggcgact ccgtgatcgc cggagtcgcc
      961 tggcagccgg gcaccggggt ctccggcgtc gaggtgcagg tggacgacgg cgactggcag
     1021 ccggccgaac tcgcgaccgc gatcgccgac gacacctggg tgcagtggcg cgtgccctgg
     1081 aaggcgaagg ccggtgagca cagcatccgc tgccgggcga tcgccggcga cggcacggtg
     1141 cagaccgaca cgatcgcgcc cccggcgccg gacggcgcga gcggctggca cacgatcagc
     1201 gtcagcgtct cctgatcgat cggcgacggc ccgtgtgagg gttccgtgag gatcgcactt
     1261 cacagtattc acgaattcgt gaaatgggtg taccgtcgcc tctcatgaac accatcatca
     1321 gcacgcgcgg tctgaccaag acctacggtc gcgtgcacgc cctcgacgga ctcgacctcg
     1381 aggtggaagc cggccaggtg cacggcttcc tcggcccgaa cggcgcggga aagtccacca
     1441 ccatccgcat cctgttgggg ctcgcccgca agtcgggcgg cacggcatcc gtcttcggcc
     1501 tcgagccctg gggcgacgcc gtgcagatcc atcgtcgcgt cgcgtacgtg cccggcgacg
     1561 tcagcgtctg gccgaacctc tccggcgggg aggcgatcga cttcctcgcc cgcctgcgcg
     1621 ggacgaagac caaggatgcc gcgtaccgcg ccgagcggga gcgcctgatg accgcattcc
     1681 agttcgaccc gcgcaagaag gggcgcgcgt actcgaaggg caaccgccag aaggtcgccc
     1741 tcatcgccgc gttcgccgtg ccggccgacc tgtacatcct cgacgagccg accagcggcc
     1801 tggacccgct catggaggtc gtgttccgcg atgaggtgca gcgactgcgc cgcagcggcg
     1861 ccactgtgct gctctccagc cacatcctct ccgaggtgga gctgctctgc gaccgggtct
     1921 cgatcatccg cgagggaaag atcgtcgagt cgggtacgct ctccgaactg cgccacctca
     1981 cgcgcaccga ggtgtcgttc gaggctgcat ccgccgacgc cctcgccggc atccccgagg
     2041 cgcacgacgg cacggtcgag gagggccgcg cgcgcttcac ggtcgacagc gactcgatcc
     2101 cttccgtgct ccccgtgctc gcccagcgcg ccgtcaccgg gctgcggatc gaaccgccct
     2161 ccctcgagga gctgttcctg cggcactacg gcgacgacct cgccgccctg cgcgccgccg
     2221 aggaagactc ttcggatgcc ggtgccggca cccgcgcctc acggcgcgcg gccgcgaagg
     2281 ggacgcgctg atgttccgct cgcttctgcg ccagcgattg cgccgcgact ggctgcagct
     2341 gctgctctgg atcctcgcga cagtgctgat ggcgtactcg ggcttcgcgg gcgtcgccca
     2401 gtcgtacgcc acgcacaagg accgggtcga ggtgctcgcc gccgtcatgg cgaacccggt
     2461 gatcatgatg ttccgcgggc ttccgtccgg ggcatccgag ggacagttcc tgtccttcga
     2521 gatcctcccc tggctcacca tgctcgccgc cctgatgagc accttcctcg ctgtccgcca
     2581 cacccgcggc gatgaggagg cgggccgcac cgagttgctc gccgccacac ccgcgggccg
     2641 ggcgctgccg accgtcgcca cgatcgtcca cggcctggca gcgaacgtcg tgctcggcgt
     2701 gctggtcgcg ttcgcactgg ccggctcgaa gctgcccgcc gcaggctcct ggctgacggg
     2761 cgcggtgtcg gccacgacgg gcatcgcgtt cctcggcatc ggcctcatcg ccgcgcagct
     2821 catgcgcacc tcgcgcggtg cgaactccct caccgtctgg atcctggtcg gaaccttcct
     2881 gatcaacggg atcggcaatg tcgcaggcac gccgagcgac gacctcactc gcatctcgag
     2941 ttcgggactc gtctggctct cgcccttcgg gtgggccgag caggcccgcc ccttcgacac
     3001 cgacgatgcg tggcccgccc tgctcgggct cggcttcggg ctcgtgctcg ccgtcgggtc
     3061 ggtggctctg cagtccctgc gcgacatcgg cgagggcttc atccccgccc gccccggacg
     3121 ggtcttcgcc cgacccgccc tgtccagccc gcatgcgctg gtctggcggc tcacgaacgg
     3181 cgcgatcatc ggctgggctg taggcggtgc gctcacgggc atcctcgcga cgaagctctc
     3241 cggcctggcc gggcagatct ccggccagaa tcccgcggtg cagcagatcc tcgacaagat
     3301 cggcaaggcc ggcaaccttg aggagaccgt ggtctcggtg ttcttcacga tgatgggcat
     3361 ccttgccgcg tgcgccgccg tgcagatcct cgcccgcgcg cgacaggagg aggcgcacgg
     3421 caccgccgag atcgtgctgg cacagcccgt gggtcgcgtg cgctggctgc tggactacct
     3481 gctcgtggcg acgatcgcga tcgcgatcat cgtcgccgcg accatgctcg ccgcattcgc
     3541 cggcctcgcc gggaacgagg atgcctcggc gctggcgaag gtcgtgaccg tcgtcgggct
     3601 cggccagggc ctcgccgcgt gcgtgttcac ggcgctcacg gccctggcgt tcgtgctgct
     3661 cccccgcggc acgatcgcca tcgcctgggc actcgtgatg ctggcgacgg tcttcggcct
     3721 gttcggcccg ctgttcggtc tgcccgaatg gacgacgcgc ttctcgccgt tcgccgtgac
     3781 gcccatcatc tcggacggga ccaccgacct gcgcggcacg tggtggctgg tgatcatcgt
     3841 cgcggtcgtg gccacggcat ccctcagtct catgcgccgg cgcgagctgc gccccgcagc
     3901 gtgaccgctg cgccggtggc cgcccacgga tcggccaccg gcatccggat gaaaggatga
     3961 acgcatgagc gaggacgtga cgcacgcggg cgtggatgcc gccgagagcg cagccgccgc
     4021 gatcaccgcg gtcggcttcc cgcggatgcc cgcgcgcgtc atgatggcgc tgatcgccgc
     4081 cccctccgag gggtacacgg catccgagct cggcgaccgg ctgggcgtca gcgccgccgc
     4141 ggtctcgggt gccgtgcgct acctgcagac gctgcacgtg atccgcaagg tctcgcgtcc
     4201 ggaccagcgg ctggcgcgtt acgagatcat ccccgacacc tggtacgtga tgatgtcgaa
     4261 caactccccc gtctacaacc ggctcgccgc ctacatcgac gagatcggcg cggcgcacgc
     4321 cgaggatcac gcggcccgcc tgcgcgccga ggagatggcc gagttcttcc ggttcatgtc
     4381 cctgcggatg cctcagctgg tcgacgagtg ggagacgctg cgcgccgagc gccggctgca
     4441 gagctgagag caccggcccg gcgtgcgcgc tcgggaactc gtcgacgagt gggagacgct
     4501 gcgcgccgag cgtcgcctgc agagctgaga gcaaccccct gtcccatcgg ggatgtgctg
     4561 tataccgtgg agggcatgag cggaaacagc agcgagccgg atccgatgac caccgcaggg
     4621 aacgaagcgg gtacttcgcg acgcgaacct caggtgctga gatgggtctt ctggcccgcc
     4681 gccgtgatcg tcctggcgtt cgccctgttc gctctgctcg cgccacacat cgcagaggcg
     4741 ctcttcaaga ccgtccagac gacgatcgtg aacgccttca actggtacta cgtgctgatc
     4801 gccgccttct tcgtggcgtt ctgcctcttc ctcggcttca gcaggttcgg cgacatcaag
     4861 ctgggccgcg acgacgagga gcccgagttc tcgctgatga gctggttctc cctgctgttc
     4921 gcggcgggaa tgggcatcgg cctggtcttc tacggggtca gcgaaccgct ctcgcacttc
     4981 acgaatccgc gccccggagt caccggcaca ccggagcagc tggcccaggc cgcgctcggc
     5041 cagacctacc tgcactgggg tgtgcacgcc tggtcgatct acgtggtgat cggactcgcc
     5101 ctggcctacg ccatccaccg gcggcgacgt cccgtgtcga tccggtggac cctggagccg
     5161 ctgctcggca agcgcgtcga gggtggctgg gggcacgcga tcgacgtgat cgccctggtc
     5221 ggcaccctgt tcggcgtcgc cacctcgctg ggactcggcg tgctgcagat gagcgccggc
     5281 ctgcactccg ccggcttcgg gaaccccgac gagaccaccc aggtggtgct catcctgatc
     5341 atctcggtgt tcgtgctgat gtcggtgctg tccggcgtga ccaagggcat gaagtggctg
     5401 tcgaatgcga acctcgtgct cgccggcctg ctggtgatct atctgctcgc ggcggggccg
     5461 acggagttcc tgctgcgcga gttcgtgcag tccctcggct actacatcca gaacttcgtg
     5521 ggcctctcct tcaacgtcag cgcgttccag ggcgccgccg gcgagcagtg gcaggcgtcc
     5581 tggacgtcgt tctactgggg ctggtggatc tcgtgggcgc cgttcgtggg gatcttcatc
     5641 gcccgcgtct cgaaggggcg cacggtgcgc gagttcgtga tcggggtgat cctggtgccc
     5701 accctgatcg gcgtgctgtg gttctcagtt ctcgggggct cggccctggc gatggagctc
     5761 gcgaagcccg gctccctcac cggccccgac ggaacggtcg atctgcaggg cgcgctgttc
     5821 gcgatgctgc agaacgtccc cggtgccacc gtggtgagca tcggcgccat cctgctgctc
     5881 gcgatcttct tcatcacctc ggccgattcc ggggcactgg tgatgggcat gatcgccacc
     5941 ggcggcaagc ccgagccgaa gcgctggatc cgcacgttct tcgtcgtgat caccgcactg
     6001 ctggcgatct ccctgctgct gtcgggcggg ctcaccgcgc tgcagaccgc ggccatcacc
     6061 atcgcgctgc cgttcagcat cgtgatgctg ctgatctgct ggtcgaccgt ggtcgcgttc
     6121 acccgcgaac ggcgtgccta cgccaaggcc gaacgggcgc agttcatcga ccgcatcggc
     6181 gagtactacg gtctcgaggt cgagtcgcac gacgcgaccg gcgtgctcgg cgcccaaccg
     6241 cgctggatgc ggcgactgca acgccggttc gggctgcccg tggcgccgac cgagccgatc
     6301 cgcatcacac ccggagcgct ctcgctggag agccacggcg atgcctcgcc ctccaccctc
     6361 gacgtcgacg atctggtggg cggcgatgag ctcgccggcc tcgacgatcc cgacgtgaag
     6421 cagcaggatg cgggtccgtt cctgaaagag tgacgcctgt caacgccctc cccgcggcga
     6481 gcgccaccgg ctaccgtcac gggcatggac gaacggcaga gcagtcagga tcaggacgcc
     6541 gggcagcagc cgcacacgcc ggggcaggat ccggcgggca gcggcgaacc gcacgaggca
     6601 cctcagtcgg cggatgctcc ggatgggagc gagggcgcgc acaccggcat ccccgaggac
     6661 aacccggcgc acaacaccgc ccccgacggc atgcccggcg atgaggaggg tgcgccgctc
     6721 ggcggggatg agcgcaccga ggagcagctc gacgccgaca ccgcggtcga gcgcgatgcg
     6781 ctgaagagcc tcgacccgga tgacacgccg gcctgaggcg tcctgaacgc acttcggcgc
     6841 cggcggtgag aaccgccggc gccgatgggg ccttctgaag ccgggtcagg ccccgcgcag
     6901 ctccagggtg cagcacttga tgccgccgcc gccgagcagc agctcggata ggtcgaccat
     6961 gatcgggttg tagccgcgct cgcgcagctg cttctcgaag cctgtggcgc gcggcgagat
     7021 gatcacgttg tagccgtcgg aagccgagtt gagaccgaac acggcgccgt cctcatcggc
     7081 gacgaggatc gcgtcgggga agcgctcctc gaggatgcga cgggatgcct cgtcgaaggc
     7141 gttcggcagg taggcgacgt tggcgtgctc cgggccgccg ttctcctcac cgacgaccgg
     7201 gtcgagcacg gtcagcgcgg tgtcgaggtg gtagaagcgc gggtcgacca gggtgagcgt
     7261 cacgacctcg cggtcgaaga cctcgtgcac ctcgcggtgg ctgtcgcccg cggagcggaa
     7321 gccggtaccg gccaggatca cgtcgccggc gagcaggaag tcaccctcgc cctcgttgac
     7381 ctcctggggc agcacggtgt cgaagccgtg ggtgcggaac cactcggcga acgcgggcgc
     7441 ctcgccctga cgctgctcgt agtagaactc gggcacgtac gccttgccac cgatggtgaa
     7501 gccgccgttg gccgtgtaca ccatgtcggg gtagccggcg agcggctcga tgagctcgac
     7561 ctcgtgaccg agctccaggt acaggtcgta cagcttctgc cactgcgcga cggccttggc
     7621 ggtgtcggtc ggtgcggccg ggtccatcca ggggttgatc ttgtagtcga ccgtgaagtg
     7681 ctcgggccgg cacatcaggt agcggcggtg gtgggccttg cgggtcgcgg tgccggtggc
     7741 ggcggtctca ggcgtggaca tgggtgctcc tctggagaag gtgcggcgga cgctgtccag
     7801 gggcccggcg gaagttcgac ccgcccacgg tgagtggagc gcggggaaga tgcgacacag
     7861 gcacgccagt tgtattttcg catccggatg ccgagatcgc cacttttttc gctgagagtc
     7921 gtgtcgggcg gtgtccgctc agatcacttc cgcgctccag ggatccgggg tgccgaagcg
     7981 atgcgcggtt atcgagatcg cctgctcgtg cacgaacggc agcagctcga gccggccggc
     8041 cgaggtcacc tcgttgtcgt acacggcgag gtcggggtct ccctcgacgg cgtccgcgag
     8101 cgcgcggtgc aggtcggcca cggcatcctg cccgccgatc agacgcaccc ggcccacgcg
     8161 ggtcgcctcc ggcgcaggac ggcggatccg ctcgatccac tcctcatcgc tctccaccgc
     8221 gacgggcacc tcgagcgcgc cgagctcggc gcgcaccccc gcgggcacgc cgtcgggcac
     8281 cgacacgaag aagtccgccc cggagcggat gccggcgagc accacccgca ggatcgcggc
     8341 gagggatgca tccgacgcgg cccgcaccgt caccggcagc ggccggtacc ggaacaggtt
     8401 gcgctccacg cccagctgcg agacgtcgcg cacctgaccg aactcgcggt cccaggcgac
     8461 ggcatccgag agcgccgcct gccgcagcca ctcgaaggag tcgaagtcga gcgagggctg
     8521 cgccgactcg atgagtccgg agatcttcac gtccagcccg cgcagatgca gcgtgctcga
     8581 cgacttgccc cgcgggcgcg agcgccagga gcccagcccg atcaggtagt tcggaccgcc
     8641 ggccttcgca ccggggccga ccgaggagcg cttccacccg ccgaacggct gacgctgcac
     8701 gatcgcgccg gtgatcccgc ggttcacgta caggttgccg gcctgcacct cgtccagcca
     8761 cagcgccaga tcggcggggt cctgggtgtg caggcccgcg gtcagtccgt acgccacggc
     8821 gttctgcatc cggatcgcct cggacagcgt cgaggcgtgc atgatgccga gcaccggccc
     8881 gaagaactcc tcgcggtggg tgcgagaacc gggctgaacg cccacgcgga ttccgggact
     8941 ccacagcctt ccgctgtcgt ccagtgagcg cggctcgacc agccactgct cctcgccctc
     9001 gagctgggtg agcgcccagg ccagcttgcc ctgcggcttc tcgatcaccg ggcccacctc
     9061 ggcctgcgga tccccgggcc aggccacgcg cagcgagcgg gtggcgtcga ccagctgccg
     9121 cgcgaagcgc ttggagcggc ccaccggccc gaccaggatc gccagggacg ccgccgagca
     9181 cttctgcccg gcgtgcccga acgcgctgtg caccaggtcg gcgacggcga ggtcgaggtc
     9241 ggcggtcggg gtgatgatca tggcgttctt gccgctggtc tccgcgagca gcggcaggtc
     9301 gctccgccac gaccggaaga gcgccgcggt gtcccacgag cccgtgagga tcacgcgatc
     9361 cacgtccggg tgcgagatca gctgctgccc gagctcgccc tcctccacgt cggcgagcac
     9421 cagcacgtcg cgcgggatgc cggcctgcca cagcgcctcg gcgatgaccg ccgc
//
LOCUS       DATMSX010000524         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_140509,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000524 DATMSX010000000
VERSION     DATMSX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140509"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN041_16760"
     CDS             <1..228
                     /locus_tag="VN041_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30735.1"
                     /translation="ARTVAASLPETGAEGILSSLRDRDDVTELRVDADRLTLRAADSD
                     AVAAALLAAGAHDLEITAPTLETAFTALTED"
     gene            231..1043
                     /locus_tag="VN041_16765"
     CDS             231..1043
                     /locus_tag="VN041_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30736.1"
                     /translation="MTTSTPAFTRTPRALRVPRKGISSTMLRIEALRQLRNPYTLAFT
                     LAMPVVMYILFGASAAYGAQSAGHGNVSFYVMISMAAYGTAVAMSSLTSLAATESKQG
                     WGRQLAMTPLGTVGYALTKLITSVTFAALALVAVFTAGLLTGAKADDAWRWVAAAAII
                     LGIGLMYGLFGLGVGLFFNADSAAALASISMTFFAFFGNVFMPLDGVMLSIARFTPLY
                     GFVALSRWPLTDGVLTTGQVDPLWMPLLNVVVWLTLFSVLVVAGVRRSRTRR"
     gene            1070..>1901
                     /locus_tag="VN041_16770"
     CDS             1070..>1901
                     /locus_tag="VN041_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HWU30737.1"
                     /translation="MMRETDETAAGRDAEAIRAAAGSLPATWMPKRGDSRPKSPWDRF
                     GWLMAVIWLVFLAYPVIALLGSTAPEGWIIVGWAAMAAFVTLYVLGFMRGMSSGGGLG
                     RPPAPGQWLFFAGLIACAVISIPAEGGSALSFLPFIMSFASYGLNRIAHWVTFAGAVT
                     VTVLWILLMPGGLSYVSVLAIIGLLGVVNTVSTWLIIRSADADRLGLELATSEGREAV
                     ARDVHDLIGHSLTVVKLKAQLARRLIDTEPERAKAELAAIESLTAEAIAGVRATVAGA
                     R"
BASE COUNT          240 a          672 c          661 g          328 t
ORIGIN      
        1 gccaggacgg tcgccgcgag cctgccggag accggcgccg agggcatcct ctcctccctg
       61 cgcgatcgtg acgacgtgac cgagctgcgc gtggacgcgg atcggctcac cctgcgcgcg
      121 gccgactcgg atgccgtggc cgctgcgctc ctcgccgcgg gagcgcacga tctggagatc
      181 acggctccca ccctcgaaac cgccttcacc gccctgacgg aggactgacc atgaccacct
      241 cgacccccgc cttcacccgc acgccgcgtg ctctgcgcgt cccgcgcaag ggcatctcat
      301 cgaccatgct ccgcatcgag gcgctgcgcc agctgcgcaa cccgtacacg ctcgcgttca
      361 ccctggcgat gccggtggtg atgtacatcc tcttcggcgc cagcgccgcg tacggggcgc
      421 agtccgcagg ccacggcaac gtgtccttct acgtgatgat ctccatggcc gcctacggca
      481 cagccgtcgc gatgagctca ctgacctcac tggccgccac cgagtccaag caggggtggg
      541 gtcgtcagct ggccatgacg ccgctcggaa cggtcggcta cgccctgacc aagctcatca
      601 cctcggtgac gttcgccgcc ctcgcgctgg tcgcggtgtt cacggccggc ctgctcaccg
      661 gagcgaaggc ggacgacgcc tggcgctggg tcgcggccgc cgcgatcatc ctcggcatcg
      721 ggctgatgta cggcctgttc gggctgggcg tcggactgtt cttcaacgcc gactccgcag
      781 ccgcactggc atccatctcg atgaccttct tcgccttctt cggcaacgtg ttcatgcccc
      841 tggacggcgt gatgctgtcg atcgcccgct tcaccccgct ctacggcttc gtggctctca
      901 gccggtggcc gctcaccgac ggcgtgctca ccacggggca ggtcgacccg ctctggatgc
      961 cgctgctgaa cgtggtcgtc tggctcaccc tgttctcggt gctggtcgtc gccggggtgc
     1021 gccgctcgcg gacacgccgg tgacacgcgc gcgtcagtag gttggactca tgatgcggga
     1081 gaccgacgag acggccgcgg gccgggacgc cgaggcgatc cgcgccgcgg ccggttcgct
     1141 gcccgccacg tggatgccga agcggggaga cagccgaccg aagagcccct gggaccggtt
     1201 cggctggctg atggccgtga tctggctggt cttcctggcg tatcccgtga tcgcgctgct
     1261 gggctcgacg gcgcccgagg ggtggatcat cgtcggctgg gcggcgatgg ccgccttcgt
     1321 caccctgtac gtcctcggct tcatgcgggg gatgagctcg ggaggcggtc tcgggcgtcc
     1381 gccggcgccc ggccagtggc tgttcttcgc cgggctgatc gcctgcgcgg tgatctcgat
     1441 cccggctgag ggcggctcgg cgctgagctt cctgcctttc atcatgtcgt tcgcgtccta
     1501 cgggctgaac cggatcgcgc actgggtgac cttcgccggt gcggtgaccg tgaccgtgct
     1561 gtggatcctc ctgatgccgg gagggctgag ctatgtctcg gtgctcgcga tcatcggcct
     1621 gctgggcgtg gtgaacacgg tctcgacgtg gctgatcatc cgctccgccg acgccgatcg
     1681 gctggggctc gaactggcga ccagcgaggg ccgtgaggcc gtggcgcgtg acgtgcacga
     1741 cctgatcggg cactcgctga ccgtggtgaa gctcaaggcg cagctcgccc gccgtctgat
     1801 cgacaccgag cccgagcgcg ccaaggccga gctcgccgcg atcgagtcgc tgacggcgga
     1861 ggcgatcgcg ggcgtgcggg cgacggtggc gggcgcacgg g
//
LOCUS       DATMSX010000525        12755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_149769,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000525 DATMSX010000000
VERSION     DATMSX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149769"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1059
                     /locus_tag="VN041_16775"
     CDS             367..1059
                     /locus_tag="VN041_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWU30738.1"
                     /translation="MTLQTVILAAGMGSRLGRALPKPLTELSDGRTIMQQQHDNIRAA
                     FGRDARITAVVGYRAETIIEAFPSANYVHNERYDVTNTSKSLLRALSATGKGGVLWMN
                     GDVVFDPRILGRAIEYIERDQSFVTVNTSKVSDEEVKYTVTAEGFIKELSKTVKGGLG
                     EAVGINYISSADKKAFMRQLQRVEDQDYFERGLELSIAEDGLLLEPMDVSDLYAVEVD
                     FAEDLERANLFV"
     gene            1159..2151
                     /locus_tag="VN041_16780"
     CDS             1159..2151
                     /locus_tag="VN041_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30739.1"
                     /translation="MPEKVHKLHILDEPALAPVPPSVADERSFSARGLDRVLSVQRPL
                     VVAHIRSIRLRHPDATPQEIVRILETRYLAAVTTGGAAVGATAVIPGIGTGITLALSG
                     VETVGFMESTALFAQSVAEVHGIAIQDPERARALVMALMLGKEGVDLIGQLTRQASGK
                     GDTRAAYWGELVTKSLPRAAVGPIVDRLKSAFIRQFASRGGASFVGKALPFGVGAVVG
                     GTGNHLLGRRVLSTSRRAFGAAPPWLPPELEPTSGTERLERRMLHGARLVGAGVATGA
                     AGLASKTAEIASKTAEAASKSAGIAKKITRRRRKTDAVPPSDPAPEASPSTATD"
     gene            2230..2535
                     /locus_tag="VN041_16785"
     CDS             2230..2535
                     /locus_tag="VN041_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30740.1"
                     /translation="MLHSIDISQPAPPTAYCVPWVVTRGDRTHPVVMNAGKEAVDFVK
                     VLRSDAQDGRPVDLWGQVLPTETIELCLCDSNVDDVVITIAWFRQRDGLEYVWRFVV"
     gene            2586..4106
                     /locus_tag="VN041_16790"
     CDS             2586..4106
                     /locus_tag="VN041_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWU30741.1"
                     /translation="MTGASTHRAGQQAALLAEWVEVRRQINALEARAAALLGDRLALM
                     DADVAETPLHRNAIERSMVAEYTAAGRMAKGSVEHAFADARSLRESFAAVRESFQAGA
                     ITASHVREILSAASPVQQAISDGDIQPHLFGLYEAAAVEFAEAEAPARTRAHVRELPA
                     VLAPRTVVERHADALTEQCVTVRPVTDELSLLSALMPTHRAQAILDRLTAMARQQKQQ
                     DREPTLPMDEQFEAEADRSVEVITGDGTFTVDPFAWNGPLEDDPADTPDAVHAYLEMM
                     DRAIDRGPVPIILPTDTRRIDQIRTEILTDLLLSGNPGEVLGDGAENITAHIQVTVAA
                     TTLSGADDNPAQLDGTGTLHPDTARTLAGTATSWTRLFLDPDGMVTATDTYQPTAGMK
                     RFLQARDQHCRFPGCRMPVHRTQLDHTHDWAQGGQTTLDNLAHLCLTHHALKHPSIPD
                     QHRWTARQLPDWTLQWTSPNDTLHHDRPPRRVMFVPSEPEPAPFDWNAPIHGDPAF"
     gene            4187..4741
                     /locus_tag="VN041_16795"
     CDS             4187..4741
                     /locus_tag="VN041_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30742.1"
                     /translation="MTLATSTVRALGALSAALLTLGMLSACTPAPEPDPKPTKSGLFA
                     SDEEAFKAAEETYRAYTDALNEVDTSDPETFEPVFEYTSGDFEVNDRKNLSIMHAEGF
                     VMSGRTIVEDFRGTSTTGDRNTVHGSVCVNVSDADIRDSDGASIVPDDRPDRYVADVT
                     FVATRGVLSISHAETDEEKTCDAL"
     gene            5023..5586
                     /locus_tag="VN041_16800"
     CDS             5023..5586
                     /locus_tag="VN041_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30743.1"
                     /translation="MVRCIDAVKKGEPLPGPTPGEPGTPGITITDLASFSPAPGTLTG
                     EPDNLGVAGLPTNFTTDAAAHTRTGTLFGYPVTARFTPASFVFRYGDGQTRTATTAGK
                     TWEALRVLQFTPTDTSHTYAERGTYQASVDTVYTAEIDLGTGYFPISGTLTIPGPPQS
                     IQIYEARTALVAHTCDEDPTAEGCPAS"
     gene            5679..7082
                     /locus_tag="VN041_16805"
     CDS             5679..7082
                     /locus_tag="VN041_16805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWU30744.1"
                     /translation="MSKGTSMSALTETPRISRLALLVGIAAATVGIIYGYDSSNIGGA
                     LLYLRKDFSLDAAAEGWVTSTVVFGEIAGALFGGALANRIGRKPTMVLVAATFTLFSL
                     LSGIAPTLIVLVIVRILLGVTIGISVVVVPVFVAESAPTRNRGAFLVLYQVATVAGII
                     LGYLIAMSLAPTHSWRWMLGLAAIPGLVITVLLLRLPDTARWYVMMGRHAEARKALVA
                     IEGPTVDVDKEIEVITAGFATSTTSSWRDLLRGPFLRATIFVVGLGFFVQITGINAIV
                     YYSPAIFESMGYTDDVTKLGLSALVQVAGLIAVFISLMVIDRMGRRPVLLIGIATMIV
                     ANILLIGVFAVGGGRFDGALTTLGFIGLVLFTAGFTFGFGSLVWVYAGEAMPAHLRSL
                     GASVMLTADLVANVIVGLAFRPILERSGMVAFLILGIMAVAAFVFVMKLAPETKGRPL
                     EDIAQFWENGGSWTAKK"
     gene            7173..8771
                     /locus_tag="VN041_16810"
     CDS             7173..8771
                     /locus_tag="VN041_16810"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWU30745.1"
                     /translation="MESVTEKPDLSTTAAKIANLRARFNDAVVEPEKIAQQKQHAKGK
                     MTARERIEMLVDNGSFVEFDEYVRHRTTAFGMDRNRPYGDSVVTGIGTIHGRTVAVYS
                     QDFTTFGGSLGEVSGDKIIKIMEFALTSGMPVVGILDSGGARIQEGVLALSKYGEIFK
                     MNTRSSGVIPQISIIMGPAAGGAVYGPALTDFVIMVDKTSQMFVTGPDVIKTVTGEDV
                     GMEELGGAYTHNTRSGVAHYLASDEDDAIDYARTLLSFLPDNNMAELPAYASEFEWET
                     TDADQRLNTIIPDSPNQPYDIHTVIDHVVDNGEFLEVQPLFAPNIVIGFGRVEGRSVG
                     IIANQPSQMAGTLNIEAGEKASRFVRFCDAFSIPIVTLVDVPGYLPGTDQEWTGVIRR
                     GAKLIYAYAEATVPLVTVILRKAYGGAYIVMGSKQLGADVNLAWPTAEIAVMGGQGAV
                     NILYRGEIKKAEEAGEDVAAVRSRLAAEYTYGVTSPFLAAERGEIDGIIEPANTRVSI
                     AKALRALKGKRAELPAKKHGNIPL"
     gene            8785..9003
                     /locus_tag="VN041_16815"
     CDS             8785..9003
                     /locus_tag="VN041_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HWU30746.1"
                     /translation="MTEHEDAPRIEIIRGNPTEEELAALLAVVSDAYTRETDEAVADE
                     PHVSVWERTQRGLRKPLRRDIPWGRFSG"
     gene            complement(9308..10324)
                     /locus_tag="VN041_16820"
     CDS             complement(9308..10324)
                     /locus_tag="VN041_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30747.1"
                     /translation="MRRTVRSMTTALALGFALYFAARAIWWIEQPTAPLLMVAAIGLY
                     LAAVLTAVLTSSAGSVRMPVGAAVFALVASAVVPAFASIALEREMRSAPFATWYIGAI
                     GLLAVVCVVRRNPIVGWVSLGILITAASAYLGIGNAFELGLVGSITWVVVAQLLVLFW
                     DRAVRDTERLADVQRAVSAWHATQLVRQRERRVRAQRALAIAGPVLSRTVAAHGELTP
                     EEKLEARLAEGTMRDELRGAVLLNDAVRQAISDARRNGTVVTVFDEGGLDGVDEDRRS
                     EIRDELATVLASVHSNRVIIRAGRDDEIAVTVVGRAAGGAHDDDAVDLWHEIRRTADD
                     SPAD"
     gene            complement(10321..11556)
                     /locus_tag="VN041_16825"
     CDS             complement(10321..11556)
                     /locus_tag="VN041_16825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU30748.1"
                     /translation="MPAEARSIRDAWSSLPTPGEVGIGLERFTGQRMERILAIVGAIG
                     STALGIQALLGAVTRLIRLDGPHLAIMLLVFLPLLWMIIACVTGRWVKQACGAFAVAY
                     ALSLMLWPAVVDRSVQEPAAQPWIFFLVNICVVAAMLAFPVRLQFAWALGVPLVYGYV
                     RLAQGDFTQGFWITTGFDVSFTIILGMVIVSLAWMFRQVATGVDDARDMAVETYAGAA
                     AASASEEERVAMAALMHDSVLAALIAAERAASDRERTLAVAMAREALTRLANTEASIA
                     QEGSDEPVNRAQIVAELRRTLSEQGADAVVEERGVPAEIPGKAARAMVLAARQAIGNA
                     LIHAGGRGLHVVAESADADRLTLTVQDTGPGFDPDAIGEDRLGIRASIIARMAAVAGS
                     SRITSDDRGTVVELSWEPS"
     gene            complement(11546..12208)
                     /locus_tag="VN041_16830"
     CDS             complement(11546..12208)
                     /locus_tag="VN041_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30749.1"
                     /translation="MSTVALIDDHESVRLGLEAACLRDGEQDVVFSGATVREYLAWRA
                     ASGARPADVVVLDLTLGDGTTVTENVATLVADGASVVIHSVADRPASVREALVAGAAG
                     VVSKSSALDDVLDAIRTVARGEALNNVEWASAVDGDREFADAQLSTREREVLRLYATG
                     LPLKAVAERLGVAYSTAKENITRIRVKYVEVGRPAPTKVDLLRRAMEDGIVAPEGLTS
                     AG"
     gene            complement(12239..>12755)
                     /locus_tag="VN041_16835"
     CDS             complement(12239..>12755)
                     /locus_tag="VN041_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HWU30750.1"
                     /translation="GGFWQVHPQAADTLHQAVGDALAGRVDADATHFDLYGGVGLFAA
                     TLGEQGASDIVTVESDRRATEHAQQNLAAFDAQAVTARVDRFLAGLPEGTRAGAVVLD
                     PPRAGAGRGVVESIHALEPEAIVYVACDPVALARDLGTFRGLGWGVTSLQAFDLFPHS
                     HHFEAVALLSR"
BASE COUNT         2168 a         4667 c         4009 g         1911 t
ORIGIN      
        1 tccggggcgt cgcggcacgc ttcgccggcg tgcgcgctgg cttcttcgcg gctgcggggg
       61 cagccgcggc gtccgcgctt cccggctggg aggtgtcgtc gcggtcggcc ccggagtcga
      121 gagaagctgt cacgggtact acggtatcaa gcggcaggca atgttcccgg agcggggtca
      181 tggtcggaaa ccgccgaatt cggccacgcg gaaccctgag tgttacgtgt gcgaaccatc
      241 acgaaacagc aacggcgacc acagcgaccc gggtcttccc aggtgtgatc agtagcatgt
      301 tcctggcacg aagaggtgtc ccgtcgatga atcgacagtg aacacaagtt ctttttagga
      361 gaaaccgtga ctcttcagac cgtcatcctt gcagcaggca tgggctcgcg cctcggtcgc
      421 gcgctgccca agccgctcac cgagctcagc gacggccgca cgatcatgca gcagcagcac
      481 gacaacatcc gtgctgcgtt cggacgcgac gcccgcatca ccgccgtcgt cggctaccgt
      541 gccgagacga tcatcgaggc cttcccgtcg gcgaactacg tgcacaacga gcgctacgac
      601 gtgaccaaca cctccaagag cctgctgcgc gcactgagcg cgaccggcaa gggcggcgtg
      661 ctctggatga acggcgacgt ggtcttcgac ccgcgcatcc tgggccgtgc gatcgagtac
      721 atcgagcgcg accagtcgtt cgtcaccgtc aacacctcca aggtgagcga cgaggaggtc
      781 aagtacaccg tgaccgccga gggcttcatc aaggagctgt cgaagacggt caagggcggt
      841 ctcggcgagg ccgtgggcat caactacatc tcgtccgcag acaagaaggc gttcatgcgc
      901 cagctgcagc gcgtcgagga ccaggactac ttcgagcgcg gcctcgagct gtcgatcgcc
      961 gaggacggcc tgctgctcga gccgatggac gtctccgacc tgtacgcggt cgaggtcgac
     1021 ttcgccgagg acctggagcg cgcgaacctc ttcgtgtgac ctccgggccg ccgagcctgc
     1081 cgaagcactg aatgccccgc cctgtcaagg ggcggggctt ttcggcgttc tcgcggatgc
     1141 cgcgcatagg atcaccccat gccagagaag gtccacaagc tccacatcct ggacgagccc
     1201 gcgctcgccc cggtgccacc gagcgtcgcc gacgagcgct cgttctcggc gcgcggcctc
     1261 gaccgcgtcc tctcggtgca gcgtccgctc gtcgtcgcgc acatccgcag catccgtctt
     1321 cggcatccgg acgcgacgcc gcaggagatc gtgcggatcc tcgagacccg ctatctcgcc
     1381 gccgtgacca ccggcggcgc ggcggtcggc gcgaccgcgg tgatccccgg catcggcacg
     1441 ggaatcacgc tggctctgtc cggtgtcgag acggtcgggt tcatggaatc gacggcgctg
     1501 ttcgctcagt cggtcgccga ggtccatggc atcgcgatcc aggatcccga gcgcgcacgc
     1561 gcactggtca tggcgctgat gctgggcaag gagggcgtcg atctgatcgg gcagctgaca
     1621 cgccaggcgt cgggcaaggg cgacacgcgc gcggcctact ggggcgagct ggtcacgaag
     1681 tcgctgcccc gcgccgcggt cggtccgatc gtggacaggc tgaagtcggc gttcatccgg
     1741 cagttcgcct cgcgcggcgg tgcatcgttc gtcggcaagg cgctgccgtt cggcgtgggc
     1801 gccgtggtgg gaggtacggg aaaccatctg ctcggcaggc gggtgctgag cacgtcgcgg
     1861 cgtgccttcg gcgccgctcc cccgtggttg ccaccggagc tggagccgac atccgggacc
     1921 gaacggctgg aacggcggat gctgcacggc gcgcgcctgg tcggtgcggg cgtcgcgaca
     1981 ggtgccgccg gcctcgcctc gaagacggct gagatcgcgt cgaagacagc ggaggccgct
     2041 tccaagtcgg cggggatcgc gaagaagatc acccgcaggc gccggaagac ggatgcggtg
     2101 ccgccaagcg atcccgcgcc ggaggcatcg ccctccacag cgaccgactg atcgcgactt
     2161 ctgcacagtc agggcgggcg tagctccgtc ctgaccggtg gcgtccgtcc ccgttcctac
     2221 cgtggcggga tgctgcattc catcgacatc tcccagcctg ctccgccgac cgcctactgc
     2281 gtgccgtggg tggtgacgcg cggcgaccgc acgcatccgg tcgtgatgaa cgccggcaag
     2341 gaggcggtgg acttcgtgaa ggtgctgcgc agcgacgcgc aggacggccg gccggtcgat
     2401 ctgtggggcc aggtactacc caccgagacc atcgagctgt gcctgtgcga ctcgaacgtg
     2461 gacgacgtcg tgatcacgat cgcgtggttc cgccagcgtg atgggctgga gtacgtgtgg
     2521 cggttcgtgg tgtgagatgc cgtggatctc ttccctcttt cgaagttttg ttctaaactg
     2581 gatgcatgac gggcgcttcc actcaccgtg cgggtcagca ggctgcgctg ctggccgagt
     2641 gggtggaggt gcgtcgtcag atcaacgcgc tcgaagcacg tgcggcggct cttctgggtg
     2701 accggctggc gctgatggat gccgatgtgg ccgagacccc attgcaccgg aatgcgatcg
     2761 aacggtcgat ggtcgccgag tacaccgcgg cggggcggat ggcgaaaggc agcgtggagc
     2821 acgcgttcgc ggatgcgcgc agccttcggg agtcgttcgc tgcagtgcgg gagtcgttcc
     2881 aggccggggc gatcaccgca tcccacgtcc gggagatcct cagcgcggct tccccggtgc
     2941 agcaggcgat cagcgacggc gatatccaac cccacctgtt cgggttgtat gaggcggcgg
     3001 ctgtggagtt cgccgaggcg gaagcaccgg cccgcacccg tgcgcacgtc cgggagctcc
     3061 ccgcggtcct cgcaccgcgc accgtggtcg agcggcatgc ggatgcgctg accgagcagt
     3121 gcgtgacagt ccgtcccgtc acagacgaac tgtcgctgct gagcgcgttg atgcccaccc
     3181 accgggccca ggccatcctg gaccgtctca ccgcgatggc cagacagcag aagcagcagg
     3241 atcgggagcc gaccctcccg atggatgagc agttcgaagc cgaagccgac cggtccgtgg
     3301 aagtcatcac cggcgacggg acgttcactg tcgacccgtt cgcctggaac ggtcccctcg
     3361 aggacgaccc cgccgacacc ccggacgccg tgcacgcgta cctggagatg atggaccgcg
     3421 ccatcgaccg cggtccggtc ccgatcatcc tccccaccga tacccgccgc atcgaccaga
     3481 tccgcaccga gatcctcacc gacctgctcc tcagcgggaa cccgggtgag gtgctcggcg
     3541 acggggcgga gaacatcacc gcccacatcc aggtcaccgt cgccgccacc accctcagcg
     3601 gcgcggacga caatcccgcg cagctcgacg ggaccggcac cctccacccc gacaccgccc
     3661 gcaccctcgc cggaacggcg acctcctgga cccggctgtt cctcgacccc gacggcatgg
     3721 tcaccgcaac agacacctac cagcccaccg ccggcatgaa acggttccta caggctcgag
     3781 accagcactg ccggttcccc ggatgccgta tgcccgtcca ccgcacccaa ctcgaccaca
     3841 cacacgactg ggcacaaggc ggccagacca ccctcgacaa cctcgcccac ctctgcctca
     3901 cccaccacgc cctgaagcac cccagcatcc ccgaccaaca ccgctggacc gccagacagc
     3961 tccccgactg gaccctccaa tggaccagcc ccaacgacac cctccaccac gaccgaccac
     4021 cacgccgcgt catgttcgta ccctcagaac ctgaaccggc cccgttcgac tggaatgctc
     4081 cgatccacgg cgaccccgcc ttctgacgtt cgaagcacga acgcgtccag cacctgcctg
     4141 tggataactc ctgcggcgac gtgccggttc gggttaccgt ggccgggtga ctctcgccac
     4201 ctccaccgtc cgcgcgctcg gcgcattgtc cgccgcgctg ctcacgctcg ggatgctgtc
     4261 ggcatgcacc cccgcgccgg agcccgaccc gaagcccacg aagtcgggac tgttcgcgtc
     4321 ggacgaggag gcattcaagg cggcggagga gacgtatcgg gcctacacgg atgcgctgaa
     4381 tgaggttgat acaagcgatc cggagacctt cgagcctgta ttcgagtaca cctctggcga
     4441 tttcgaggtc aacgacagaa agaacctcag cattatgcat gcggagggtt tcgtgatgtc
     4501 gggacggacg atcgtcgagg acttcagggg tacgtcaacc acgggtgacc gcaacacggt
     4561 tcatggttcc gtctgcgtca acgtcagtga cgcggatatc agggactcgg atggcgcctc
     4621 catcgttccc gacgacaggc ctgatcgcta tgtcgccgat gtaactttcg ttgccacacg
     4681 aggagtcttg agtatctcgc atgctgaaac cgacgaggag aagacgtgcg acgccttatg
     4741 accgctctcc tcatcgtcgg cgcgttgttt tgggcaccgc catcctttgc ggaatcatcg
     4801 cctcccccgt ccgaaccaaa gtcgggcaca agcggaacgg aatttgtggc gggcgtcggc
     4861 aaggacacac tcaacgtagc tgcgaagaag gaaacacagc atcagacagg ctcccagccc
     4921 gaatcctcgc acggtaaagg gacgacacgg acggtagccg acccgaagac gccgccacgg
     4981 aagccgaccc cgttcgagca gtgcaacacc aactggaacc tcatggtccg ttgcatcgac
     5041 gccgtcaaga agggcgagcc gctaccgggg ccgactccag gagaacccgg cacacccggc
     5101 atcacgatca ccgacctcgc gtccttctcc cccgcacccg gcactctcac cggcgaaccc
     5161 gacaacctcg gcgtcgcggg actgcccacc aacttcacca ccgacgcggc cgcgcacacc
     5221 cgcacgggga cactcttcgg ctacccggtc acggcccggt tcaccccggc atccttcgtc
     5281 ttccgctacg gcgacggaca gacccgcacc gccacgaccg ccggaaagac ctgggaggcg
     5341 ctgcgcgtgc tccagttcac gcccaccgac accagccaca cctacgcgga gcgcggcacc
     5401 taccaggcat ccgtcgacac ggtctacacc gcggagatcg acctcggcac gggctacttc
     5461 cccatcagcg gaaccctcac catccctggt ccgccgcaga gcatccagat ctacgaggcc
     5521 cgcaccgccc tcgtcgcaca cacctgcgac gaggatccga ccgccgaggg ctgcccggcc
     5581 tcctgacacg cccggcccgt ttgtgagggc gactgcgcat tgacaacccc gcagcccgag
     5641 ggacagtctc gcttcacagc gaatcttcag ctacatcgct gtcgaaggga acgagcatga
     5701 gcgcactcac cgagacacca cgcatctcac gattggcgct cctggtcggc atcgccgcgg
     5761 ccaccgtcgg catcatctac ggctacgact cctccaacat cggcggagca ctgctctatc
     5821 tgcgcaagga cttcagcctc gacgccgccg ccgagggctg ggtcaccagc accgtcgtct
     5881 tcggcgagat cgccggcgcg ctgttcggcg gagcactcgc caaccgcatc ggccgcaaac
     5941 cgaccatggt gctcgtcgcc gccacgttca cgctcttctc actgctcagc ggcatcgccc
     6001 cgacgctcat cgtcctggtc atcgtgcgca tcctgctcgg cgtcaccatc ggcatctcgg
     6061 ttgtcgtcgt ccccgtgttc gtcgcggaat cggccccgac acgcaaccgc ggcgccttcc
     6121 tcgtgctcta ccaggtcgcc accgtcgccg gaatcattct cggctacctg atcgcgatgt
     6181 ccctcgcccc cacccacagc tggcgctgga tgctgggact cgccgcgatc cccggcctcg
     6241 tgatcaccgt cctcctgctc cgcctccccg acacggcccg ctggtacgtg atgatgggcc
     6301 ggcacgccga ggccaggaag gccctcgtcg cgatcgaggg ccccaccgtc gacgtcgaca
     6361 aggagatcga ggtcatcacc gccggcttcg ccaccagcac cacctcctcg tggcgcgatc
     6421 tgcttcgcgg accgttcctg cgggccacga tcttcgtcgt cggcctcggc ttcttcgtgc
     6481 agatcaccgg catcaacgcg atcgtctatt acagccccgc catcttcgag tcgatgggct
     6541 acaccgacga cgtgaccaag ctgggcctct ccgccctcgt gcaggtcgcg ggcctgatcg
     6601 ccgtgttcat ctcgctcatg gtgatcgacc ggatgggccg ccgccccgtg ctgctcatcg
     6661 gcatcgcgac catgatcgtc gccaacatcc tgctcatcgg ggtcttcgcc gtcggcggcg
     6721 gcagattcga cggcgccctg accacgctcg gcttcatcgg actggtgctg ttcaccgccg
     6781 gcttcacctt cggcttcgga tcgctcgtct gggtctacgc cggcgaggcg atgcccgccc
     6841 acctgcgctc cctcggcgcc agcgtcatgc tcactgccga cctcgtcgcg aacgtgatcg
     6901 tcggcctcgc cttccgcccg atcctcgagc ggagcggcat ggtcgccttc ctcatcctcg
     6961 gcatcatggc cgtggccgcc ttcgtgttcg tgatgaagct cgcccccgag accaaggggc
     7021 gcccactcga ggacatcgcc cagttctggg agaacggcgg cagctggacg gcgaagaagt
     7081 gatccggatg ccggagaccg gagcgcatcg ccaaaggatc gccgaacccg ttgtggccgg
     7141 catccaacga gacgacgaga cccaccgata gggtggaatc cgtgacggaa aagcctgacc
     7201 tctcgaccac tgccgcgaag atcgccaacc tccgcgcgcg cttcaacgac gcagtcgtcg
     7261 agccggagaa gatcgcccag cagaagcagc acgccaaggg caagatgacc gcccgcgagc
     7321 gcatcgagat gctcgtcgac aacggcagct tcgtcgagtt cgacgagtac gtgcggcacc
     7381 gcaccaccgc cttcggcatg gacaggaacc gtccctacgg cgactccgtc gtcaccggca
     7441 tcggcaccat ccacggccgg accgtcgcgg tctactcgca ggacttcacc accttcggtg
     7501 gctcgctcgg cgaggtctcc ggcgacaaga tcatcaagat catggagttc gccctgacca
     7561 gcgggatgcc cgtggtcggc atcctcgact ccggtggagc ccgcatccag gagggcgtgc
     7621 tggccctcag caagtacggc gagatcttca agatgaacac ccgctcctcg ggcgtgatcc
     7681 cccagatctc gatcatcatg ggaccggccg ccggcggcgc cgtgtacggc cccgccctca
     7741 ccgacttcgt catcatggtc gacaagacca gccagatgtt cgtcaccggc cccgatgtca
     7801 tcaagaccgt caccggcgag gacgtcggca tggaggagct cggcggcgcc tacacgcaca
     7861 acacccgctc cggtgtcgcg cactacctcg cctccgacga ggacgacgcg atcgactacg
     7921 cgcgcaccct gctcagcttc ctgcccgaca acaacatggc cgagctcccg gcgtacgcct
     7981 cggagttcga gtgggagacc acggatgccg atcagcgcct gaacacgatc atcccggact
     8041 cccccaacca gccctatgac atccacacgg tcatcgatca cgtcgtcgac aacggcgagt
     8101 tcctcgaggt gcagcccctg ttcgcaccga acatcgtgat cggcttcggc cgcgtcgagg
     8161 gccgctcggt gggcatcatc gccaaccagc cgtcgcagat ggccggcacc ctgaacatcg
     8221 aggcgggcga gaaggccagc cgcttcgtgc gcttctgcga cgccttctcc atcccgatcg
     8281 tcaccctcgt cgacgtgccc ggctacctcc ccggcaccga ccaggagtgg accggcgtga
     8341 tccgtcgtgg cgccaagctc atctacgcct acgccgaggc caccgtgccc ctggtcaccg
     8401 tcatcctgcg caaggcttac ggcggcgcgt acatcgtgat gggctccaag cagctcggcg
     8461 ccgacgtgaa cctcgcctgg cccaccgccg agatcgccgt catgggcggc cagggcgccg
     8521 tcaacatcct ctaccgcggt gagatcaaga aggcggagga ggccggtgag gacgtcgccg
     8581 ccgtgcgcag ccgcctcgcc gctgagtaca cctatggcgt caccagcccg ttcctcgcag
     8641 cagagcgcgg cgagatcgac ggcatcatcg agccggcgaa cacccgcgtc tcgatcgcga
     8701 aggccctgcg cgcgctcaag ggcaagcgcg ccgagctgcc cgccaagaag cacggcaaca
     8761 tcccgctgtg accggcgagc catcatgacc gagcacgaag acgccccccg catcgagatc
     8821 atccgcggga accccaccga ggaggagctg gcagctctcc tcgcggtggt cagcgacgcc
     8881 tacacccgcg agaccgacga ggccgtcgcc gacgagccgc acgtgtccgt gtgggagcgc
     8941 acgcagcgcg gactccgcaa acccctacgt cgggatatcc cctggggccg tttctctggc
     9001 tgaacagcta aagcggatat ggactttcgt cgaaaagccg ccgattttgt cccccgaaat
     9061 acctacagac agagttattg acagccgcca gacttatatc aacgcttcgc gttaggcggc
     9121 tgctctgccc cagggtaggc agacgcagta ccgcccctag cggacagttt tcgggtagct
     9181 gttccgcact ggcgaggggc gggcggcttc gccgcccctc gcccactctc ttcgaaagtc
     9241 gtgtcccgcc ggagtccagc cggcgggatt tcgcttttca gcgtcggtgt atcagggcgc
     9301 gggcgaatca gtccgcgggt gaatcgtccg cggtgcggcg gatctcatgc catagatcga
     9361 cggcatcgtc gtcgtgcgcg ccgccagccg cacgccccac gaccgtcacg gcgatctcgt
     9421 cgtcgcgccc cgcgcggatg atcacccggt tcgaatgcac gctcgccagc accgtcgcga
     9481 gttcatcgcg gatctcgctg cggcgatcct catccacacc gtcgagtccg ccctcgtcga
     9541 acacggtcac gacggtcccg ttcctgcgag catccgagat ggcctggcgc acggcgtcat
     9601 tgagaagaac cgccccgcgc agctcgtccc gcatcgtgcc ctccgccagc cgggcctcca
     9661 gcttctcctc cggggtcagc tcgccatgcg ccgccacggt ccgcgagagc acgggccctg
     9721 cgatcgcgag cgcccgctgc gcacgcacgc gccgttcccg ctgccggacg agctgcgtcg
     9781 catgccaggc ggacaccgcc cgctgcacgt ccgccaggcg ctccgtgtcc cggacggcgc
     9841 gatcccagaa gagcaccagc agctgcgcca ccaccaccca cgtgatcgac cccacgagcc
     9901 cgagctcgaa ggcgttgccg atgcccaggt aggcggatgc cgcggtgatc aggatgccca
     9961 gcgacaccca gcccacgatc ggattccgcc gcaccacgca gacgacggcc agcagcccga
    10021 tcgcgccgat gtaccaggtc gcgaatggcg cgctgcgcat ctcccgctcc agtgcgatgc
    10081 tcgcgaaggc gggcaccacg gcgctcgcga ccagcgcgaa cacggctgcc cccaccggca
    10141 ttcgcacgga ccccgccgac gacgtgagca cggcggtcag caccgccgcg aggtacagtc
    10201 cgatggccgc gaccatcagc agcggcgcgg tgggctgctc gatccaccag atcgcacgcg
    10261 cggcgaagta cagcgcgaac cccagggcga gcgcggtggt catgctgcgt acggtgcgcc
    10321 tcatgatggc tcccagctca gctcgacgac ggtgccgcgg tcatctgacg tgatccgtga
    10381 ggagccggcg acggccgcca tgcgcgcgat gatcgaggcg cggatgccca ggcggtcctc
    10441 gccgatggca tccggatcga accccggccc ggtgtcctgc accgtgaggg tcagccgatc
    10501 ggcatccgcc gactccgcca ccacatgcag tccgcgacca cccgcgtgga tcagggcgtt
    10561 gccgatcgcc tgccgcgccg ccagcaccat cgcccgtgcc gccttgccgg ggatctccgc
    10621 cggcacaccg cgctcctcga ccacggcatc cgccccctgc tcagacaggg tccgacgcag
    10681 ctccgcgacg atctgggcgc ggttcaccgg ctcatcactg ccctcctggg cgatcgacgc
    10741 ctcggtgttg gccagccgcg tcagcgcctc gcgcgccatc gcgaccgcga gcgtgcgctc
    10801 ccggtcgctc gcggcccgct cggcggcgat cagcgcagcc agcacgctgt cgtgcatcag
    10861 cgccgccatc gcgacgcgct cctcctcgga cgcggatgcc gcggccgcgc ccgcgtacgt
    10921 ctcaacggcc atgtcgcgtg cgtcgtcgac gccggtcgcc acctgccgga acatccacgc
    10981 cagggacacg atcaccatgc cgaggatgat cgtgaacgac acgtcgaatc cggtggtgat
    11041 ccagaagccc tgcgtgaagt cgccctgcgc gagccgcacg tagccgtaca cgagcggcac
    11101 gccgagtgcc caggcgaact gcagccgcac ggggaacgcc agcatcgccg ccaccacgca
    11161 gatgttcacc aggaagaaga tccacggctg agcggccggc tcctggaccg agcgatccac
    11221 caccgcgggc cacagcatca gcgacagcgc gtaggccacg gcgaacgctc cgcacgcctg
    11281 cttgacccat cgtccggtca cgcacgcgat gatcatccac agcagaggca ggaacaccag
    11341 cagcatgatc gccaggtgcg gcccgtccag ccggatcagg cgggtcacgg cgccgagcag
    11401 cgcctggatg ccgagcgccg tcgagccgat cgccccgacg atggcgagga tgcgctccat
    11461 gcgctggccg gtgaaccgct ccagcccgat gcccacctcg ccgggggtcg ggaggctgct
    11521 ccaggcgtct cggatgctgc gggcttcagc cggcactcgt caggccctcc ggcgcgacga
    11581 tgccgtcctc catcgcccgg cgcagcagat cgaccttggt gggtgcaggg cgcccgacct
    11641 cgacgtactt cacccggatg cgggtgatgt tctccttcgc ggtcgagtac gccacaccca
    11701 gccgctccgc gaccgccttg agaggcagcc ccgtcgcata caggcgcagc acctcgcgct
    11761 cgcgggtgga cagctgggca tcggcgaact cgcggtcacc gtccacggcg ctggcccact
    11821 cgacgttgtt cagcgcctcg ccgcgagcga cggtgcggat cgcgtcgagc acgtcgtcca
    11881 gcgcggacga cttgctgacc acgcccgccg ctcccgcgac gagcgcctcc cgcacggatg
    11941 ccggacgatc ggcgacgctg tggatgacca cactcgcgcc gtcggccacc agcgtcgcga
    12001 cgttctcggt gacggtcgtg ccgtcgccga gcgtgagatc cagcaccacc acgtcggcag
    12061 gtcgggcgcc tgacgccgca cgccaggcga ggtactcccg gacggtggcg ccggagaaga
    12121 cgacgtcctg ctcgccgtca cgcagacagg cggcctcgag tcccagacga accgactcgt
    12181 ggtcgtcgat gagggcgacg gtgctcatgc cgccagccta cccagtgccc gcctggactc
    12241 agcgcgagag cagggcgaca gcctcgaagt ggtgcgagtg cgggaacagg tcgaacgcct
    12301 gcaacgaggt cacgccccag cccagtccgc ggaacgtgcc gaggtccctc gccagagcga
    12361 ccggatcgca cgccacgtag acgatcgcct ccggttcgag ggcgtggatg gactcgacga
    12421 ccccgcgtcc cgcgccggcg cgcggcggat cgagcaccac ggcgcccgcc ctggtgcctt
    12481 cgggaagacc ggcgaggaag cggtccaccc gagcggtcac ggcctgcgcg tcgaacgccg
    12541 cgaggttctg ctgggcgtgc tcggtggcgc gacggtcgga ctcgacggtg acgatgtcgc
    12601 tcgcgccctg ctcccccagc gtcgccgcga acagcccgac gcctccgtac aggtcgaaat
    12661 gcgtggcatc cgcgtcgacg cgaccggcga gcgcatcgcc gaccgcctgg tgcagcgtgt
    12721 cggcggcctg cggatgcacc tgccagaacc cgccg
//
LOCUS       DATMSX010000526         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_122038,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000526 DATMSX010000000
VERSION     DATMSX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122038"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VN041_16840"
     CDS             <1..936
                     /locus_tag="VN041_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30751.1"
                     /translation="PGRRSPRPDWRKRGEIALLSGPALIMFVGFVILPVAMAAFYGFF
                     KWNGFGWPTEFIGLDNYRMMWEDETFRQAVLHNGVIVVLSLLLQGPVAILFALLLNQK
                     IRGRSIIRVLIFVPYVISEVIVGIGWSLMLQDAGALNSLLEKWGLGFLQQSWIADPSI
                     AMGSLMIIITWKYLGFAVILMLAGMQSIPEELYEAAAIDGAGFWKIQWSITVPLLGPT
                     IRIWAFLSIIGSLQLFDLVNIIWGQYIAATAGTSTMATYMYQNGHLAGSYGYGNAVAV
                     MLFLITLIVALVYQRFVLRRDTEGALTGDGGRRRK"
     gene            966..1826
                     /locus_tag="VN041_16845"
     CDS             966..1826
                     /locus_tag="VN041_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30752.1"
                     /translation="MRAISPKGRHTREADVKWGSPAVYLVALVLITICITPVLYVIIG
                     GFRTNSQITLDPSGFPSPWQLGNYGDVLASSEFWTAMLNSTISAAGTTAGAVVLGVMA
                     SYVLARYEFAGRGVMYALFAAGLMFPVTVAITPLYILIRNLGLVNSLAGIILPQIAFA
                     LPTTIIILVPFLRAIPKELEEAASIDGASRLGFFWRMVVPLSLPGVITVGILAFVGAW
                     NGYMLPLFILNDQSLFTLPLGVQNFASQYSVDTARVLAYTSLSMLPALVFFSMFERRI
                     VGGLTGAVKG"
     gene            1830..4283
                     /locus_tag="VN041_16850"
     CDS             1830..4283
                     /locus_tag="VN041_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU30753.1"
                     /translation="MLQETDVTTYAPEGAVTIPAFPTASPRVSALLAEMTLEEKSAQL
                     VGYWLDQGGEVVAPMQNEMGAMRETGALAEITRHGIGHYTRVYGTRPVDPVERAAWLW
                     QEQRRLRSETRLGIPALVHEECLTGLAAWKAATFPTPLAWGASFDPELVADMGALVGE
                     SMRSLGIHQGLAPVLDVVRDPRWGRVDECIGEDPYLVGTVGAAYVRGLQSAGVHATLK
                     HFLGYSGSRAGRNHAPVSAGAREVADVFLPPFEMAIRDGGARGVMNSYAEIDGVPVAS
                     DPALLTGLLRERLGFDGVVVADYFAVAFLEIMHAVAADRGEAAQLALTAGIDVELPSG
                     DAYLAPLVERVRAGLLDEAYLDRAVIRVLLQKEQLGLLDADAFEEDPPARVDLDTPRH
                     RAVARRLAAESLVLLGNDGVLPLRGARRIALVGPNADRTAALQGCYSFANHVLAHHPD
                     HDLGFRIPTVREALTAALPDAELVHVDGCEVEGEDRSGFDTAVAAAADADVAVVVVGD
                     QAGLFGRGTVGEGNDVDSLDLPGVQRELVEALVATGTPVVMVLVTGRPYAIEWALNGD
                     VARPAAVLQAFFPGEAGGTAIADVLIGDVSPSGRMPVSLPRSSGSQPYSYLHPILGGP
                     SAVTALDSTPLRPFGFGLSYTTFAYDDFSVSEDAATDGFFTASVVVTNTGDRDGADVV
                     QLYGRDVSASLTRPVAQLLAYARVELAAGESRTVRFEVPAARFAFTNHGLTRVVEPGD
                     VHVWVASHAEASAGGEISDATGGAISNEREVVRREIAGIATDARVVALTGDVHEVTAA
                     DARIVSVVVEETSVAAEPR"
     gene            4293..>4615
                     /locus_tag="VN041_16855"
     CDS             4293..>4615
                     /locus_tag="VN041_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30754.1"
                     /translation="MNERATTVEGVRRTNLGEVLRLVHHHGARSRATITADTGLNRST
                     VADLVSELATRGLVVEQEPDATRRVGRPSPIVAPSPDVVAIAVNPEVDAIELGAISLG
                     GVVRER"
BASE COUNT          633 a         1562 c         1637 g          783 t
ORIGIN      
        1 ccggggcggc gctcgccgcg gcccgactgg cgcaagcgcg gcgagatcgc gctcctctcg
       61 ggaccggccc tcatcatgtt cgtcggcttc gtcatcctcc cggtggcgat ggcggcgttc
      121 tacggcttct tcaagtggaa cggcttcggc tggccgacgg agttcatcgg cctcgacaac
      181 taccgcatga tgtgggagga cgagaccttc cggcaggcgg tgctgcacaa cggcgtcatc
      241 gtcgtgctct cactgctgct gcagggaccg gtggcgatcc tgttcgcgct actgctgaac
      301 cagaagatcc gcggcaggtc gatcatccgc gtgctgatct tcgtgcccta cgtcatctcc
      361 gaggtcatcg tcggcatcgg ctggagcctg atgctgcagg atgccggcgc gctgaactcc
      421 ctcctggaga agtgggggct cgggttcctg cagcagtcct ggatcgccga tccctcgatc
      481 gcgatgggct cgctgatgat catcatcacc tggaagtacc tcggcttcgc ggtgatcctc
      541 atgctcgccg gcatgcagtc gatcccggag gagctgtacg aggccgccgc gatcgacggc
      601 gccggcttct ggaagatcca gtggagcatc acggtgcccc tgctggggcc gaccatccgg
      661 atctgggcgt tcctgtccat catcggctcg ctgcagcttt tcgacctggt caacatcatc
      721 tggggtcagt acatcgctgc gaccgccgga acctccacga tggcgaccta catgtaccag
      781 aacggtcatc tggccggcag ctacggatac ggcaacgccg tcgcggtgat gctcttcctg
      841 atcaccctga tcgtggctct cgtctaccag cgcttcgtgc tgcgtcgtga caccgagggt
      901 gcgctgaccg gcgacggcgg aaggaggcgc aagtgagcgc cgagacactg aacacccgcg
      961 tcgccatccg tgcgatctcc ccgaagggcc gccacacgcg cgaagccgac gtgaagtggg
     1021 ggagccccgc ggtctacctg gtcgcgctgg tgctgatcac gatctgcatc acgccggtgc
     1081 tgtacgtgat catcggcgga ttccgcacca actcgcagat cacgctcgat ccctccggct
     1141 tcccgtcccc gtggcagctc ggcaactacg gcgacgtgct ggccagctcc gagttctgga
     1201 cggccatgct gaactccacg atctccgccg cgggcaccac ggccggcgcg gtcgtgctcg
     1261 gcgtgatggc gagctacgtg ctcgcccgct acgagttcgc gggtcgcggc gtgatgtacg
     1321 cgctgttcgc ggccggcctg atgttcccgg tgacggtggc catcacgccg ctgtacatcc
     1381 tgatccgcaa tctggggctg gtgaacagcc tggccgggat catcctgccg cagatcgcgt
     1441 tcgcgctgcc gacgacgatc atcatcctcg tcccgttcct gcgcgccatt ccgaaggagc
     1501 tcgaggaggc cgcgtccatc gacggcgcgt cgcgtctggg cttcttctgg cggatggtgg
     1561 tgccgctcag cctgcccggt gtgatcaccg tcggcatcct ggcgttcgtg ggcgcctgga
     1621 acgggtacat gctgccgctg ttcatcctca acgaccagag cctgttcacc ctgccgctgg
     1681 gcgtgcagaa cttcgcgtcg cagtactcgg tggacaccgc gcgcgtgctg gcgtacacct
     1741 cgctgtcgat gctgcctgcg ctggtcttct tcagcatgtt cgagcgccgc atcgtcggcg
     1801 gactgaccgg agcggtgaag ggctgacgga tgctgcagga gaccgatgtg accacctacg
     1861 caccggaggg tgcggtgacg atccccgcct ttccgaccgc ctctccgcgg gtgagtgcgc
     1921 tgctggccga gatgaccctg gaggagaagt ccgcgcagct ggtgggctac tggctcgacc
     1981 agggcggcga ggtcgtcgcc ccgatgcaga acgagatggg cgcgatgcgc gagaccggag
     2041 cgctcgccga gatcacccgg cacgggatcg ggcattacac ccgcgtctac ggcacgcgtc
     2101 cggtcgatcc ggtggagcgg gcggcatggc tgtggcagga gcagcgccgg ctcaggagcg
     2161 agacccggct gggcatcccc gcgctcgtgc acgaggagtg cctgacgggg ctggcggcgt
     2221 ggaaggcggc gaccttcccc acgccgctgg cctggggcgc gtccttcgac cccgagctgg
     2281 tcgcggacat gggtgcgctc gtcggggagt cgatgcgcag cctcggcatc catcagggcc
     2341 tcgccccggt gctcgatgtc gtgcgagacc cgcgctgggg acgcgtcgac gagtgcatcg
     2401 gcgaggatcc gtacctggtc ggcaccgtcg gtgcggccta tgtgcgggga ctgcagtccg
     2461 cgggcgtgca cgccacgctg aagcacttcc tgggctactc gggctctcgg gccggccgca
     2521 accacgcccc ggtctcggcc ggcgccaggg aggtcgcaga cgtgttcctc ccgccgttcg
     2581 agatggcgat ccgggacggc ggagcgcgtg gcgtgatgaa cagctacgcc gagatcgacg
     2641 gggtgcccgt ggcatccgac ccggcgctgc tgaccggact gctccgggag cgactcggct
     2701 tcgacggtgt cgtggtggcg gactacttcg ccgtggcgtt cctcgagatc atgcacgcgg
     2761 tggccgcgga tcgcggtgag gccgcgcagc tggccctgac cgccggcatc gacgtcgagc
     2821 tcccctccgg tgacgcgtac ctggcgccgc tggtcgagcg cgtgcgcgcg gggctgctcg
     2881 atgaggccta tctggatcgc gccgtgatcc gcgtgctgct gcagaaggag cagctcgggc
     2941 tgctcgacgc cgacgcgttc gaggaggatc cgcccgcacg ggtcgacctc gacacccctc
     3001 ggcatcgcgc ggtggcccgc cggctggcgg cggagtcgct cgtgctgctc ggcaacgacg
     3061 gcgtcctgcc gctccgcggt gcgcgtcgca tcgccctggt cgggccgaac gccgaccgca
     3121 cggcggcact gcagggatgc tattcgttcg ccaaccatgt gctcgcgcat caccccgacc
     3181 acgacctcgg cttccgcatc ccgaccgtcc gcgaggcgct gacggcggcg cttcccgatg
     3241 ccgagctcgt gcacgtcgac ggttgcgaag tcgagggcga ggaccggtcc ggtttcgata
     3301 cggcggtcgc cgcggcggcg gatgccgatg tcgcggtggt cgtcgtcggc gatcaggccg
     3361 ggctgttcgg ccgcggcacg gtcggcgagg gcaatgacgt ggactcgctc gaccttccgg
     3421 gtgtgcagcg tgaactggtc gaggccctgg tcgccacggg gacgccggtc gtgatggtgc
     3481 tcgtcacggg gcggccctat gccatcgagt gggcgctgaa cggagacgtc gcacgcccgg
     3541 cggccgtgct gcaggcgttc ttccccggag aggcgggcgg cacggcgatc gccgatgtgc
     3601 tgatcggaga cgtctcgcca tcaggtcgga tgccggtgag cctgccgcgc tcgtccggat
     3661 cgcagccgta cagctatctc cacccgatcc tcggcgggcc gtcggcggtc accgcgctcg
     3721 actcgacgcc gctgcggccg ttcggcttcg gcctgtcgta cacgacgttc gcgtacgacg
     3781 acttctcggt gtcggaggat gccgcgaccg acggattctt cacggcatcc gtcgtggtga
     3841 cgaacaccgg cgaccgggac ggtgcggatg tcgtgcagct gtacggcagg gacgtctcgg
     3901 catccctcac gcgccccgtc gcccagctgc tcgcgtatgc acgcgtcgaa ctcgccgcgg
     3961 gcgagagccg caccgtgcgg ttcgaggttc ccgcggcgcg cttcgcgttc acgaaccacg
     4021 gcctcacgcg cgtcgtggag ccgggggacg tgcacgtgtg ggtcgcctct cacgccgagg
     4081 cgtcggccgg cggcgagatc tccgatgcga ccggtggcgc catctcgaac gagcgcgagg
     4141 tcgtgcgccg cgagatcgcg ggtatcgcga ccgacgcgcg cgtcgtcgcg ctgaccggag
     4201 acgtgcacga ggtgacggcc gcggacgcgc gaatcgtgtc ggtcgtggtc gaagagacga
     4261 gcgttgcggc ggagcctcga tagcctgggc ggatgaacga acgagcgacg acggtggagg
     4321 gcgtgcgccg cacgaacctg ggcgaggtcc tgcggctggt gcaccaccac ggcgcgcgct
     4381 cgcgcgcgac gatcacggct gacaccggat tgaaccgttc gaccgtggcc gatctcgtgt
     4441 cggaactggc cacgcgcggt ctcgtcgtcg agcaggagcc ggacgccacg cgccgcgtgg
     4501 ggcgtccgtc tccgatcgtc gcacccagtc ccgacgtcgt cgcgatcgcg gtcaaccccg
     4561 aggtcgatgc gatcgagctc ggtgcgatct cgctcggcgg cgtcgtccgc gagcg
//
LOCUS       DATMSX010000527         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_93661,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000527 DATMSX010000000
VERSION     DATMSX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93661"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VN041_16860"
     CDS             complement(<1..570)
                     /locus_tag="VN041_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWU30755.1"
                     /translation="MPSPAFDDYLKTVYAHTEWQDAPITPSQLATALGIAPSSVTEMV
                     KKLSAAGLVSHQPYGAVRLTDAGRERALQMLRRHRLIETWLVREFGYAWHEVHDEAEV
                     LEHTISDRLLEGIDQRLGRPRFDPHGDAIPDAEGRVDREPFVLLADAIDGHSGRVLRV
                     SDRDPELLQALEDAGVAVGVELTASPDGVR"
     gene            complement(572..898)
                     /locus_tag="VN041_16865"
     CDS             complement(572..898)
                     /locus_tag="VN041_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30756.1"
                     /translation="MPSAPAKKHRDDSQARRAMRLCTWFALIAGALGVVLIAWPRLLP
                     AGGPWVQLVLGAMTLFLAFRARAIGSRGVNDYDGRLSLLAAMAGFATMFFAGSAAFGV
                     LAALGG"
     gene            1061..3601
                     /locus_tag="VN041_16870"
     CDS             1061..3601
                     /locus_tag="VN041_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HWU30757.1"
                     /translation="MNPRSLPFRMSLAVVAVGCLLAGGVQSASASAFAAAPQPRSSAP
                     QLETLDRGLVAVSTSDGVFLSWRLLASEVTGTTGTGQAGPDFAVYRDGERVAVVTDST
                     NYADADGSASSEYTVAPVVNGVELAASDPVTAWAQGHHDLPLQKPADGVTPKGDAYTY
                     SANDVSVGDVDGDGQYEYVVKWDPSNSKDVSQRGYTGNVYLDTYELDGTLLNRLDLGV
                     NIRAGAHYTQFLVYDFDGDGRAETMLKTAPGTKTVRYSDDGSIASERYITMPKSDVKA
                     GYSNTDDYRLSAADYEQHLVDMFLGWSDRPEVRSGQWPATLEEAWGMPVTHEYPLSRA
                     DAEELADTFIDVYAPSRSAKNLLREFEGFIVDGPEYLSVFDSATGKELQTIAYPTERG
                     DDGLLWGDYAMARIEPANRVDRFLSGVGYFDGQHPSAVFARGYYTRTTITTLDWDGKR
                     LTQRWQVDSGQVPMTNPFNDSPHGRDGTDPVYGGITTQGDHSLSLADVDSDGRQELVY
                     GSATIDDDGSLLYSSHDVLPEGSADPGADVRLGHGDAMHVTDIDPSRSGLEIWTAHEG
                     GAYAPYGSVLRDAATGEALFGAYSGKDTGRAMIGDVRPDVPGIEVWSSMPGGTDASGL
                     LSAKGEILSTATPGTNMSIRWAGDLTTQIINGSGPQTPTIDDWTRGRLLTADGTLTNN
                     GTKGNPSLVADVLGDWREELLVRTADSSALRFYTTTEPTSHKLATLMQDVQYRVETAR
                     QQTVYNQPAYTSFYLASDIDWAKAPVLTAAVTPAAPVFKDRPGTAHDEVQLPRVAGIT
                     FYVNGVKADAANAKVRVTGEVSVVAVPHAWYRIADGAASQWTATLGDR"
     gene            complement(3692..4969)
                     /locus_tag="VN041_16875"
     CDS             complement(3692..4969)
                     /locus_tag="VN041_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWU30758.1"
                     /translation="MPKSARIEEHGVDQEVPRRGIRVPWTGLSLLGPALVAGVAYLDP
                     GNVASNMTAGARYGYLLVWVVLAGNVMAWLIQYLSAKLGVVTGRSLPEVLGARIRNPW
                     ARRAYWLQAELVAMATDIAEVLGGAVALNLLFGIPLLLGGVITGTVSIVLLAVHSRRG
                     ARHFEFVIIGLMALIVVGFVAGLVVAPPDPAGVVGGMLPRFADSGSVLLAASILGATI
                     MPHAIYAHSALARDRFPATVSRSHSIRTLLTATRWDVTIAMLIAGSVNLAILLVAAAN
                     LAGVEGTDSLEGAHAAITTNLGPLIGTLFAVGLLASGLASTSVGAYAGAEIMHGLLTV
                     RIPLLARRLVTLIPALVILGVGFDPTLALVLSQVVLSFGIPFALIPLVALTAQRRTLG
                     EHANRRLTTLLGILFSVLIIALNAVLLWLVATG"
     gene            5183..5821
                     /locus_tag="VN041_16880"
     CDS             5183..5821
                     /locus_tag="VN041_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWU30759.1"
                     /translation="MEEQAPDPTPERTTHGVGPWQGEWPVEDWYDPELLEHGDTRNVI
                     DRYRYWRMEVIVADLDLQRHPFHVAIENWQHDMNIGSIVRSANAFLADTVHIIGRRRW
                     NKRGAMVTDRYQHVVHHPDVESFAAWAAGEGIPIIAVDNVGDAVPVDRAELPERCVLL
                     FGQEGPGLSEEALTAASGHIEITQYGSTRSINASAAAAVVMYEWCRRYARQA"
BASE COUNT          974 a         2041 c         2093 g          800 t
ORIGIN      
        1 gcggacgccg tcgggggacg cggtgagctc cacgcccacg gcgacgccgg catcctccag
       61 cgcctgcagc agctcggggt cccggtcgct cacccggagc acgcgaccgg agtggccgtc
      121 gatcgcgtcg gcgagcagca cgaagggctc ccggtcgacc cggccctcgg catccgggat
      181 cgcatcgccg tgcgggtcga atcgcgggcg tcccagccgc tggtcgatgc cctcgagcag
      241 gcggtcgctg atggtgtgct cgagcacctc ggcctcgtcg tgcacctcgt gccaggcgta
      301 gccgaactcg cgcacgagcc aggtctcgat gaggcggtgg cgacgcagca tctgcagggc
      361 gcgctcacgc ccggcatccg tcagccgcac cgcgccgtac ggctggtgcg agacgagtcc
      421 tgcggccgag agcttcttga ccatctcggt cacgctcgac ggggcgatgc cgagtgcggt
      481 cgcgagctgc gagggcgtga tcggggcgtc ctgccactcg gtgtgcgcgt agaccgtctt
      541 cagatagtcg tcgaacgcgg gggagggcac gtcagcctcc cagcgcggcg agcacgccga
      601 aggcggcgct gccggcgaag aacatggtcg cgaagccggc catcgcggcc agcagcgaca
      661 ggcggccgtc gtagtcgttc acacccctgc tgccgatcgc ccgggcgcgg aacgcaagga
      721 acagcgtcat cgcgccgagc accagctgca cccacgggcc accggcgggc agcaggcgcg
      781 gccaggcgat gagcaccacg cccagcgcgc cggcgatgag cgcgaaccag gtgcacagcc
      841 gcatcgcgcg acgggcctga gagtcgtcgc ggtgcttctt ggcgggggcg gagggcatgg
      901 gttcgagcct acccgggacg ttcgggcaag cggacgtgaa cctttgtaca tcgatgtaat
      961 gaatcgtctc aaatctcttg atccccgacg ctgcgcacgg tagtttcggg tcgcgcggaa
     1021 agcgctatcc aacggggccc agcccagagg agtttcaatg atgaatccca gatcccttcc
     1081 cttccgcatg tcactggcgg tggttgcggt cggctgcctg ctcgccggcg gagtgcagtc
     1141 ggcatcggca tccgccttcg ccgccgcccc gcagccgcga tccagtgcgc cgcagctgga
     1201 gacgctcgac cgcggcctcg tggccgtctc gacgagcgac ggcgtcttcc tcagttggcg
     1261 cctgctcgcg tcggaggtca ccggcaccac cggcacgggt caggccggac ctgacttcgc
     1321 cgtctaccgc gacggcgagc gggtcgcggt ggtcaccgac agcacgaact acgcggatgc
     1381 cgacggatcg gcctcgtccg agtacaccgt cgcgcccgtc gtgaacggcg tcgagctggc
     1441 ggcatccgat cccgtcaccg catgggcgca gggccatcac gacctcccgc tgcagaagcc
     1501 cgccgacggg gtcacgccga agggcgacgc gtacacgtac tccgcgaacg acgtgtcggt
     1561 gggcgatgtc gacggcgacg gccagtacga gtacgtcgtg aagtgggacc cgtccaactc
     1621 caaggacgtg tcgcagcgcg ggtacaccgg caacgtctac ctcgacacct acgagctcga
     1681 cgggaccctg ctgaaccggc tcgacctcgg cgtgaacatc cgcgccggcg cgcactacac
     1741 gcagttcctc gtctacgact tcgacggcga cggcagggcc gagaccatgc tgaagacggc
     1801 gccggggacc aagacggtcc gctactccga cgacggctcg atcgcctccg agcgctacat
     1861 caccatgccc aagagcgacg tgaaggcggg gtactcgaac accgacgact accgtctcag
     1921 cgccgccgac tacgagcagc atctcgtcga catgttcctg ggctggagcg accggcccga
     1981 ggtgcgctcc ggacagtggc ccgccacgct cgaggaggcg tggggaatgc cggtcacgca
     2041 cgagtacccg ctcagccgtg cggatgccga ggagctcgcc gacacgttca tcgacgtgta
     2101 tgcgccgagc cgcagcgcca agaacctgct gcgcgagttc gagggcttca tcgtggacgg
     2161 gcccgagtac ctgagcgtct tcgacagcgc gaccggcaag gagctgcaga ccatcgccta
     2221 ccccaccgag cgcggagacg acggcctgct ctggggcgac tacgcgatgg cccgcatcga
     2281 gccggccaac cgcgtagacc gcttcctctc gggcgtcggg tacttcgacg gacagcatcc
     2341 ttcggccgtc ttcgcccgcg gctactacac gcgcaccacg atcaccacgc tcgactggga
     2401 cggcaagcgc ctgacccagc ggtggcaggt cgacagcggt caggtgccca tgacgaaccc
     2461 gttcaacgac tcgccgcacg ggcgcgacgg caccgacccg gtctacggcg gcatcacgac
     2521 gcagggcgac cattcgctga gcctggccga cgtcgactcc gacggcaggc aggagctcgt
     2581 ctacggttcc gcgacgatcg acgacgacgg cagcctgctg tacagctcgc acgacgtcct
     2641 gcccgagggc agcgccgacc ccggcgccga cgtccgcctc ggccacggcg acgccatgca
     2701 tgtgaccgac atcgatccct cccgctccgg cctggagatc tggacggcgc acgagggcgg
     2761 cgcatacgcg ccgtacggct cggtgctgcg cgacgccgcg accggcgagg ctctgttcgg
     2821 cgcgtactcc ggcaaggaca ccggtcgcgc gatgatcggc gacgtccgtc cggacgtgcc
     2881 gggcatcgag gtgtggtcga gcatgccggg aggcacggat gccagcggac tgctgagtgc
     2941 gaagggcgag atcctctcga cggcgacgcc tggcaccaac atgtccatac gctgggccgg
     3001 cgacctgacc acgcagatca tcaacggcag cggcccccag acaccgacga tcgacgactg
     3061 gacgcgcgga cggctgctga ctgcggacgg cacgctgacc aacaacggca cgaagggcaa
     3121 cccgtcgctc gtcgccgatg tgctcggaga ctggcgtgag gagctgctcg tgcgcaccgc
     3181 ggactcgagc gcgctgcgct tctacacgac caccgagccg acgtcgcaca aactggcgac
     3241 gctgatgcag gacgtgcagt accgcgtcga gaccgcacgg cagcagacgg tgtacaacca
     3301 gcccgcgtac acgtcgttct atctcgccag cgacatcgac tgggcgaagg ctccggtgct
     3361 gaccgcggcg gtcacgcctg cggcgccggt gttcaaggac aggccgggca ccgcgcacga
     3421 cgaggtgcag ctgcctcgcg tcgccggcat cacgttctac gtgaacggcg tgaaggccga
     3481 cgcggcgaac gcgaaggtgc gcgtcaccgg cgaggtgtcg gtggtcgcgg ttccgcacgc
     3541 ctggtaccgc atcgccgacg gcgccgcgtc gcagtggacg gcgaccctcg gcgaccgctg
     3601 atcggctgac ctccgcctgg ggcacgtctc gctcccgtcg cacgagcctg tgcgacggga
     3661 gcgagtgcgt gtcctcagtc tgccgggcgg gtcagcccgt ggcgaccagc cagagcagca
     3721 ccgcgttcag ggcgatgatc agcaccgaga acaggatgcc gagcagcgtc gtcaggcggc
     3781 ggttggcgtg ttcgccgagc gtgcgccgct gggcggtcag cgcgaccagc gggatcagcg
     3841 cgaacgggat cccgaacgag agcacgacct ggctgagcac cagcgcgagc gtggggtcga
     3901 agccgactcc gaggatgacc agcgccggga tcagcgtgac gagccggcgt gcgagcagcg
     3961 ggatgcgcac ggtgaggagc ccgtgcatga tctcggcgcc cgcgtaggcg cccaccgacg
     4021 tggaggcgag gccagacgcg agcagcccga ccgcgaacag ggtgccgatg agcgggccga
     4081 ggttcgtggt gatcgccgcg tgcgcgccct cgagggagtc ggtgccctcg acccccgcca
     4141 ggttggcggc ggccaccagg aggatggcga ggttcacgga gccggcgatg agcatcgcga
     4201 tcgtcacgtc ccagcgggtg gcggtgagga gggtccggat gctgtgggag cgggagactg
     4261 tggccgggaa ccggtccctg gccagggcgc tgtgggcgta gatcgcgtgc ggcatgatcg
     4321 tcgcgccgag gatggatgcc gccagcagca cggagccgct gtccgcgaag cggggcagca
     4381 tcccgccgac gactcccgcc gggtccggcg gggcgaccac caggcctgcg acgaagccga
     4441 ccacgatcag ggccatcagg ccgatgatga cgaactcgaa atggcgggca ccgcggcgcg
     4501 agtgcacggc cagcagcacg atcgagacgg ttccggtgat cactccgccc agcagcaggg
     4561 ggatgccgaa cagcaggttc agcgcgacgg cgccgccgag cacttcggcg atgtcggtgg
     4621 ccatcgccac gagctcggcc tgcagccagt aggcgcgacg cgcccacggg ttgcggatgc
     4681 gggcgccgag gacctcgggc aggctcctgc cggtgacgac gccgagcttg gcggacaggt
     4741 actggatcag ccaggccatc acgttgcccg ccagcacgac ccagaccagc aggtagccgt
     4801 agcgtgcgcc ggcggtcatg ttgctggcga cgttcccggg atcgaggtac gcgacgccgg
     4861 cgacgagcgc cggcccgagc aggctcagac cggtccacgg cacgcggatg ccgcgtcggg
     4921 gcacctcctg gtcgaccccg tgctcctcga tccgcgctga tttaggcatg ccgaaatcct
     4981 agcctgtttt cggtatgcct aaaagtcgtg tcggggttgg ccgggtccca gttcgtcccg
     5041 ctctctgcgc cgttcggatc tccacatcgc agttcgtccc gctctgactc gcgaattccg
     5101 ggacgaactg cgacctggac gcctgcgcag ccgggaatcc ggcacgaact gcgacccggc
     5161 ggccgcccga tagcctggcg gagtggaaga gcaggccccc gacccgacac ccgagcgcac
     5221 gacgcacggc gtcggtccgt ggcaggggga gtggccggtc gaggactggt acgaccccga
     5281 gctgctggag cacggcgaca cccgcaacgt gatcgaccgg taccgctatt ggcggatgga
     5341 ggtgatcgtc gccgatctcg acctgcagcg gcatccgttc cacgtcgcca tcgagaactg
     5401 gcagcacgac atgaacatcg gctcgatcgt gcgcagcgcc aatgcgttcc tcgccgacac
     5461 cgtgcacatc atcggccgtc gccgctggaa caagcggggt gccatggtca ccgaccggta
     5521 ccagcacgtg gtgcaccacc cggacgtgga gtccttcgcc gcctgggctg ccggcgaggg
     5581 catcccgatc atcgcggtcg acaacgtggg cgacgcggtc ccggtcgacc gggccgagct
     5641 tccggagcgc tgcgtgctgc tgttcggaca ggagggcccg gggctctccg aggaggcgct
     5701 gaccgcggca tccgggcaca tcgagatcac ccagtacggg tcgacccgat cgatcaacgc
     5761 cagcgcggcg gcggccgtcg tgatgtacga gtggtgccgc cggtacgccc ggcaggcctg
     5821 atcggggtcc gtcgcgattc cgcggcggtg cagggcggac ctcacccgcg cgggtgatcc
     5881 gctcgcgcag ggcgcgtgag agcatgag
//
LOCUS       DATMSX010000528         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_55661,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000528 DATMSX010000000
VERSION     DATMSX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55661"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2766)
                     /locus_tag="VN041_16885"
     CDS             complement(<1..2766)
                     /locus_tag="VN041_16885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWU30760.1"
                     /translation="MGWHNPDLSWTELERTLSAEHPVHAEPVRPRSDPGPVSRRRSRT
                     PLTPVPRPADAVPYAELHAHSWYSFLDGTSSPEDLVAEAERLGLTALALTDHDGFYGA
                     SRFAEVAEAMKAKVQTVYGAELSLGLSAPQRGTPDPEGDHLLVIVHGEEGYHRLSGAI
                     SAAQLRGGEKGRPVYDLEELASRADGHWTVLTGCRKGAVRRGLERGDARAPLQHLIDL
                     FGHDHVAVELFDHGDPLDTRRNDALAELANRMRLPVVATGNAHYAEPAGSRLAEAVAA
                     VRAVRSMDDLDGWLPSHSGAHLRSGAEMTARFRRYPGAISHGLRIAEECAFPLHRAKP
                     ALPRQEVPEGETPMSHLRALVRAAVPQKYPTLDEKGRQRIERELDVIEEKDFPGYFLI
                     VHGIVKEAKDRGILCQGRGSAAASAVCYLLGITAVDPILYRLPFERFLATTRSEEPDI
                     DVDFDSGRREEIIQWVYQEYGRERAAQVANVIQYRPKNAVRDMARALGYSPGQQDAWS
                     KQVDGWGAGLEPVEGHDIPDDVLAYADALIRAPRHLGIHSGGMVLTDRPVGEVVPVEH
                     ARMPNRTVIQWDKDDSASMGLVKFDLLGLGMLSALRHCFDLVRESTGEEWTLETLPKE
                     EPAVYDMLCRADSIGVFQVESRAQMGLLPRLQPREFYELAIQIALIRPGPIQGGAVHP
                     FVRRKAEKDRVDAENRERLKRGEEPVPFEIPYPHPDLEDILKRTLGVPIFQEQLIQMA
                     TAIGDCTADEADLLRRAMGSKRGLEKIERVREKLYAGMRRRGLDQDVSDRIYAQIQAF
                     SNFGFAESHSLSFALLVYASSWLKLHYPAAFLAGLLRSQPMGFYSASSLTADARHHGV
                     QVLRPDLHASGVLDGLERIEDGDAPTGLDSCLITPDPPAPQRFDRSAPDESAAHRRDG
                     RFAVRLGL"
     gene            complement(2766..>3058)
                     /locus_tag="VN041_16890"
     CDS             complement(2766..>3058)
                     /locus_tag="VN041_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWU30761.1"
                     /translation="PDPLPTEVFTPLRPVRVMGAEGAPIIIGERGELSCPPALIDDSE
                     VIGWAGPWPVHEHRWDAGRSRSGHRFQLLDRQHRAWLVLHSEGAWWAEGRYR"
BASE COUNT          463 a         1076 c         1063 g          456 t
ORIGIN      
        1 cagtcccaga cgcaccgcga agcggccgtc ccggcggtgc gcggcggact cgtcgggggc
       61 ggagcggtcg aagcgctgcg gcgccggcgg atcgggagtg atcaggcagg agtccaggcc
      121 ggtcggtgca tcgccgtcct cgatccgctc gagcccgtcc agcacccccg aagcgtgcag
      181 atcgggtcgc agcacctgta cgccgtgatg acgggcgtcg gcggtgagcg acgacgccga
      241 gtagaacccc atcggctgcg agcgcagcag gcccgccagg aacgccgccg ggtagtgcag
      301 cttcagccag gagctggcgt acaccagcag cgcgaaggac aacgagtgcg actcggcgaa
      361 gccgaagttc gagaacgcct ggatctgcgc ataaatgcgg tcggagacgt cctgatccag
      421 gcctctgcgc cgcatgccgg cgtagagctt ctcccggacc ctctcgatct tctccagacc
      481 gcgcttggaa cccatcgccc ggcgcagcag gtcggcctcg tcggcggtgc agtctccgat
      541 cgcggtggcc atctggatga gctgctcctg gaagatcggc acgccgagcg tgcgcttcag
      601 gatgtcctcc agatcagggt gcggataggg gatctcgaac gggacaggct cctcgccccg
      661 cttcaggcgc tcccggttct cggcatccac ccgatccttc tcggccttgc ggcgcacgaa
      721 gggatgcacc gccccgccct ggatgggccc cggccggatg agcgcgatct ggatggcgag
      781 ctcgtagaac tcccggggct gcaggcgggg cagcagcccc atctgcgcgc gggactcgac
      841 ctggaacacc ccgatcgagt ccgcccggca cagcatgtcg tagaccgcgg gctcctcctt
      901 ggggagggtc tccagtgtcc actcctcgcc ggtggactcg cggacgagat cgaagcagtg
      961 ccggagggct gacagcattc cgagcccaag caggtcgaac ttcaccagcc ccatcgaggc
     1021 ggagtcgtcc ttgtcccact ggatcaccgt gcggttcggc atccgagcgt gctcgaccgg
     1081 caccacctcg ccgaccggac gatcagtgag caccatcccg ccggagtgga tgcccagatg
     1141 ccggggagcc cggatcagcg cgtccgcata ggcgagcacg tcgtcgggga tgtcgtgtcc
     1201 ctccaccggc tcaagaccag caccccagcc gtcgacctgc ttggaccagg cgtcctgctg
     1261 cccgggcgag tacccgagtg cccgggccat gtctcgtacg gcgttcttgg gccggtactg
     1321 gatgacgttc gcgacctgcg ccgcgcgctc ccgtccgtac tcctggtaca cccactggat
     1381 gatctcctcc cgccggccgg agtcgaagtc cacatcgatg tccggttcct cgctgcgggt
     1441 cgtggcgagg aacctctcga agggcagtct gtacaggatg gggtcgaccg ccgtgatgcc
     1501 cagcagatag cagaccgcac tcgccgccgc cgagccgcgg ccctggcaga ggatgccgcg
     1561 atccttggcc tccttcacga tcccgtgcac gatgaggaag tacccgggga agtccttctc
     1621 ctcgatgaca tccagctcgc gctcgatgcg ctgcctgccc ttctcatcca gagtcgggta
     1681 cttctgcggc acggcggccc ggacgagcgc ccgcaggtga ctcatcgggg tctcgccctc
     1741 cgggacctcc tgccggggga gggccggctt cgcccggtgc agagggaagg cgcattcctc
     1801 tgcgatgcgc aggccgtgcg atatcgctcc cggatagcgc cggaaccgag ccgtcatctc
     1861 cgcgccgctg cgcagatgcg cgccgctgtg cgagggcagc catccgtcca ggtcgtccat
     1921 gctgcgcacc gcgcgcactg ccgccaccgc ctcggcgagc cgggaacccg caggctccgc
     1981 atagtgggcg ttgcctgtgg ccaccacggg caaccgcatc cggttcgcga gctcggcgag
     2041 cgcatcgttg cggcgggtgt cgagcggatc gccgtggtcg aacagctcca cggcgacgtg
     2101 atcgtggccg aacagatcga tcaggtgctg cagcggtgct cgggcgtcgc cgcgctccag
     2161 gccgcgccgc accgcccctt tgcggcatcc ggtgagcacc gtccagtgcc cgtccgcccg
     2221 tgaggcgagc tcctcgaggt cgtagaccgg ccgccccttc tcgccgccgc gcagctgagc
     2281 ggccgagatc gccccggaga gtcggtggta accctcctcg ccgtggacga tgacgagcag
     2341 atggtcgccc tccgggtcgg gggtgccacg ctgcggagcg gagagcccca gcgacagctc
     2401 ggcgccgtac acggtctgca ccttcgcctt catcgcctcg gccacttcgg cgaaacggga
     2461 ggcgccgtag aagccgtcgt ggtcggtgag ggcgagagcg gtgaggccca gcctctctgc
     2521 ttctgcgacg agatcttcag gggaggacgt gccgtcgagg aaggagtacc aggaatgcgc
     2581 gtgcagttcc gcatagggga cggcgtcggc ggggcggggg accggggtga gaggcgtgcg
     2641 cgaacggcgc cggctgacgg gtccgggatc ggaacgtgga cgcacgggct cggcgtgcac
     2701 cggatgctcg gcgctgagcg tgcgctccag ctccgtccag gagagatccg gattgtgcca
     2761 gcccatcagc ggtacctccc ctcggcccac caggcgccct cggagtgcag caccagccag
     2821 gcccggtgct gccggtccag caactggaag cgatgcccgg agcggctccg tccggcatcc
     2881 cagcggtgct cgtgcaccgg ccagggtccc gcccagccga tcacctcgct gtcgtcgatg
     2941 agcgcaggcg ggcaggacag ctcgccgcgc tcgccgatga tgatcggcgc gccctcggca
     3001 cccatgaccc gcaccgggcg caggggcgtg aacacctcgg tcggcagcgg gtccggca
//
LOCUS       DATMSX010000529         7218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_46488,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000529 DATMSX010000000
VERSION     DATMSX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46488"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VN041_16895"
     CDS             <1..1233
                     /locus_tag="VN041_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU30762.1"
                     /translation="TGVLVRAAAALRELQPEAEGMLVRESGSLPLKARYEVAKAIAEL
                     EAAARLTTMPQGDVLPVEDPGTLGIARRIPVGVVGVIAPWNAPLTLAIRSVAPAIALG
                     NAVLVKPDIKTPFSGGALIAEIFERAGLPEGLLHVLPGDAETGEAVVSSPHTAVIAFT
                     GSSAAGRRVGEIAGRMLKRVSLELGGNNAIIVLADADLDLAVRCGVGGAFRHQGQVCM
                     ATGRHLVHERIADEYVRRLTEAVATMAPADPSEPGAALGPLISTTQAERVQTIVDQAV
                     AGSARLVTGGAHDGPFYEPTVLDDVDEQNTAFRSEIFGPVAPVTRFSSDEEALRLADG
                     TDYGLTAAIHTRDVGRALGLAQRLRVGMIAVNGQTVYDAPHIPMGGMGASGNGSRHGD
                     RWNLDEFTHWQWVTVPTT"
     gene            1373..2821
                     /locus_tag="VN041_16900"
     CDS             1373..2821
                     /locus_tag="VN041_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU30763.1"
                     /translation="MTRWDRSEADGRIFSDGWREAGGGVLPIVAPGDGSIVGRAGIAD
                     DGDVERAGQRAVAAQAEWGRASYRTRADVLHRAAEAMREIRADVEDVLIRESGSLPGK
                     ARHEVDKAIDELVEAAGLVTRSQGDILPVEDPSTLGIARRIPVGVVGVIAPWNAPLML
                     AIRSVAPAIALGNAVIVKPDVKTAFSGGAAIAEAFARAGLPDGVLHVLPGGPETGEAV
                     VRSPHTNVISFTGSSAAGKRVGEVAGGLLKRVVLELGGNNAFIVMADADLDHAVASGL
                     GGSFRHQGQICMATGRHLVHESIADEYVERMRAATERMRLGDPLSPETTLGPLITVGQ
                     AERVQGIVDAAVADGARIVRGGTHEGVFYQPTILDGVTSDNAAFRQEIFGPVAPITRF
                     STEEEALALANASDYGLSAAIHSRDVARALGFASQLRAGMVAVNGQTIYDAAHIPMGG
                     IGASGNGGRHGGHWNLDEFTYWQWVTVPTISG"
     gene            3021..4892
                     /locus_tag="VN041_16905"
     CDS             3021..4892
                     /locus_tag="VN041_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30764.1"
                     /translation="MSASQNSRSLSLPPIDDDVWPESTEVLIMGGGPVGLSCAILLAQ
                     RGIDVLLVERRDFVFRFPRAHLLNTRTMEAFHDMGVADDIYAATPQHDRWRKAVWYTT
                     ATGPGRFDGLKIGEVPAWGGGEDAERYRSSSPRAFANMPQLRLDPLIHKHAVAAAPGR
                     IRQGQEVIGIDQDDEAATVRIRDRDTGEIREVRARYVVAADGGRNSADMLGIELEGPR
                     DMREVVNYHVSLDLSSWSEPDALLGHFLHPAGGARRRGTIQALGPTHYNRDSEEWLVS
                     VAAWMIEGDPDDESALLESIRRMLGLADDHEITMHSMTRWNYNGLVAKRFRAGRVFLA
                     GDAAHKHPPTGGLGLNSGIQDVQNLCWKLSAVLHDEAPEALLDSYQAERRPIVAWYTA
                     HSLENANRHPPIAEALGFSEDEAEGFRNLEVFVGEGPESDEMRRRVAAEVQHNAHDYS
                     QLGVEAGYHYWAGAFVPDGTPAPGDESDPVAFHPTSRPGHHLPHVWLADAAGRAPRST
                     HDLVRPHGLTLLTSADAAPDWQQAVDALTGTMPVTVVAVPDDEGWRAVRQIDDDGALL
                     LRPDRKVAWRAASAPADRASALRDALEVVLRGGDAPDHDPAEPFLKRIRAAASVLVQ"
     gene            5041..6042
                     /locus_tag="VN041_16910"
     CDS             5041..6042
                     /locus_tag="VN041_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU30765.1"
                     /translation="MIIDVHGHMTAPDSYYAWKASLLAARGSHGGKPPVISDDELIAS
                     YHAPHPSFGNVSHMEHIDGAGIDLQLISPRPFHAMHSERPTRIVEWFTAETNNIIKRT
                     TELFPGRFKGIAGIPQSPYLTVEQWLPELRRAITELGFVGAMVNSDPYEGTEVPPAMG
                     ERYWYPLYETLCELDVPMLLHSAGCKFPAREPYSLHFIQEETVSVWSMIQANVFADFP
                     DLKVIVAHGGGAIPYQMGRFLPSVVRTGVSYLDKLRNFYFDSCLYTQDSLELLMKAVG
                     TDRVLFGSEKPGTGSQIDPETGRWFDDIHLLIDDIEWLDADRRADVLENNARSIFRL"
     gene            6074..7090
                     /locus_tag="VN041_16915"
     CDS             6074..7090
                     /locus_tag="VN041_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30766.1"
                     /translation="MTEIVAGFGTSHGPQLKAAPPEAWETRGVADRRSKGLKFRGGTY
                     TFDELRELREDFSHEITHDVMQRRWDSCQGSMDKVAEHIKAQDVDVLVIVSSDHKETY
                     GDEWLAPFSVFWGDEVAHVPFTQEQLDAMAPGLAEAAMGDVPDHTIIRPTHRELGKHI
                     IQSTQAEDFDTGATEIVPAGRYENHSIPHGFGFIYQRFMGEDSTVPMVPIFINTFWEP
                     NPPSAKRCYDFGRAVGRAIQSFPGDLRVGVVASGGLSHFVIDEKLDREFIEAMKNHDA
                     EYLSQVPAGEMMSGASELRNWIAVAGIADEVGLQVTGVDYEACYRTEAGTGNAMGFVT
                     WGRA"
BASE COUNT         1136 a         2570 c         2504 g         1008 t
ORIGIN      
        1 accggcgtgc tcgtccgtgc ggcggccgcg ctccgcgagc tgcagccgga agcggagggg
       61 atgctggtgc gcgagtcggg atcgctgccg ctcaaggccc gctacgaggt cgccaaggcg
      121 atcgccgaac tggaggcggc agcgcgcctg accacgatgc cgcaaggcga tgtccttccc
      181 gtcgaggacc cgggcacgct gggcatcgcc cggcgcatcc cggtcggcgt cgtcggcgtg
      241 atcgcgccct ggaacgcgcc gctgacgctt gccatccggt ctgtggcccc ggcgatcgcg
      301 ctgggcaacg ccgtgctcgt caaacccgac atcaagacgc cgttctcggg cggtgcgctg
      361 atcgccgaga tcttcgagcg cgcaggcctt cccgagggac ttctgcacgt gctcccgggg
      421 gatgccgaga ccggcgaggc cgtggtgagc tcgccgcaca cggccgtgat cgcgttcacg
      481 ggctcatccg ccgccggccg ccgggtcggt gagatcgccg ggcggatgct gaagagggtc
      541 agcctggaac tcggcgggaa caacgcgatc atcgtgctgg cggacgccga tctcgacctc
      601 gccgtgcgct gcggcgtcgg cggtgcgttc cggcaccagg ggcaggtctg catggccact
      661 ggccggcacc tcgtgcacga gcgcatcgcc gacgagtacg tgcggaggct cacggaggcg
      721 gtcgccacca tggccccggc cgacccgtcg gagcccggag ccgctctcgg accgctgatc
      781 agcaccacgc aggccgagag ggtgcagacg atcgtcgatc aggcggtcgc cggcagcgca
      841 cgactcgtga ccggaggcgc gcacgacgga cccttctacg agccgacggt gctggacgac
      901 gtggacgagc agaacaccgc cttccgctcg gagatcttcg ggccggtggc gccggtgacg
      961 cgcttctcct ccgatgagga ggcgctgcgc ctggccgacg gcacggacta cgggctgacg
     1021 gccgcgatcc acactcgcga cgtggggagg gcgctgggac tggcccagcg tctgcgcgtc
     1081 gggatgatcg ccgtcaacgg tcagaccgtg tacgacgcgc cgcacatccc gatgggcgga
     1141 atgggcgcct ccggcaacgg cagcaggcac ggggatcgtt ggaacctcga cgagttcacg
     1201 cactggcagt gggtgacagt ccccacgacg tgacgaccac gcgcccgcgt cccgtgcgac
     1261 atggtgtcca tgctgcggag ggcgcttcgc tacgcgatgc ggagccggcg cttccggcga
     1321 gcggctagca tcgcaacggt tccgctcacg cacgcacagc tggaggtcga cgatgacgcg
     1381 atgggatcgc tccgaggccg acgggcgcat cttctcggac ggctggcgcg aggcgggcgg
     1441 cggggtgctc cccatcgtcg cgcccggaga cgggagcatc gtcggcaggg ccggcatcgc
     1501 cgacgacggc gacgtcgaac gggcaggaca gcgagcggtc gcggcgcagg cggaatgggg
     1561 gcgcgcctcc taccgcacca gggccgacgt gctccatcgc gcggccgagg cgatgcgcga
     1621 gatccgggcc gacgtcgagg acgtgctgat ccgggagagc gggtccctgc caggcaaggc
     1681 caggcacgag gtggacaagg ccatcgacga gctcgtagag gcggccggcc tcgtgacccg
     1741 gtcccagggt gacatcctgc ccgtcgagga cccgagcacg ctcggcatcg cccgccgcat
     1801 cccggtcgga gtcgtcggtg tgatcgcgcc ctggaacgca ccgctcatgc tcgcgatccg
     1861 gtcggtggct cccgccatcg cgctcggcaa tgccgtcatc gtcaagcccg acgtcaagac
     1921 cgccttctcc ggcggcgcag ccatcgccga ggccttcgcc agagccggtc tccccgacgg
     1981 cgtgctgcac gtcctccccg gcgggccgga gacgggcgag gccgtcgtgc gctccccgca
     2041 cacgaacgtc atctcgttca ccggatcctc ggccgcgggc aagcgcgtcg gagaggtcgc
     2101 cggaggactg ctcaagcggg tggtgctcga gctcggcggc aacaacgcct tcatcgtcat
     2161 ggccgatgcc gacctggacc acgccgtcgc gagcggtctc ggcggctcct tccgccatca
     2221 ggggcagatc tgcatggcca ccggccggca cctcgtgcac gagagcatcg ccgacgagta
     2281 cgtcgagcgg atgcgggccg caaccgagcg gatgcgcctc ggcgacccgc tctcgcccga
     2341 gacgacgctc ggaccgctga tcaccgtcgg ccaggcggag cgcgtgcagg gcatcgtcga
     2401 cgccgccgtc gcggacggcg cacggatcgt gcggggcggc acgcacgagg gcgtcttcta
     2461 ccagcccacg atcctggacg gcgtgacctc cgacaacgcc gccttccggc aggagatctt
     2521 cggacccgtg gcgccgatca cccggttctc gacggaggag gaggccctcg ccctcgccaa
     2581 cgcgagcgac tacggcctgt ccgccgcgat ccacagccgc gacgtcgcgc gggcgctggg
     2641 cttcgcctca cagctgcggg ccggcatggt cgccgtgaac gggcagacga tctacgacgc
     2701 cgcgcacatc ccgatgggcg gcatcggcgc atccggcaac ggcggacggc acggcggcca
     2761 ctggaacctc gacgagttca cgtactggca gtgggtgacg gtgccgacga tctcggggtg
     2821 agacccggcc gtgacccggc cgcgaccagg ggcgaggcgt gcggagaggc acggctcgcc
     2881 cctttgtcgt ccccggccgg gtgcggagcc gtcgatgcga catcgtgtcg gaatcggagc
     2941 ccgagtttca cgccggactc acgtatccgg gcgggggagg cccttcataa tctggggttc
     3001 ccgcagtcga ggaggagtcc gtgtccgcat cccagaacag tcgcagcctg tccctgcccc
     3061 ccatcgatga cgacgtctgg ccggagtcga ccgaggtgct gatcatgggc ggcggccccg
     3121 tcgggctgtc ctgcgcgatc ctgctggcgc agcgcgggat cgacgtgctc ctcgtcgagc
     3181 gtcgcgactt cgtgttccgc ttcccgcgcg ctcacctcct caacacgagg accatggagg
     3241 cgttccacga catgggcgtc gccgacgaca tctacgcggc gacgccgcag cacgaccgat
     3301 ggcgcaaggc cgtctggtac accaccgcca ccggccccgg ccgcttcgac ggcctgaaga
     3361 tcggcgaggt cccggcctgg ggtggcggtg aggacgccga gcgctaccgc tcgtcgagcc
     3421 cgcgggcgtt cgcgaacatg ccgcagcttc gcctcgaccc gctcatccac aagcatgccg
     3481 tcgccgcagc tccgggcagg atccgtcagg ggcaggaggt catcggcatc gaccaggacg
     3541 atgaggccgc gaccgtccgg atccgcgacc gggacaccgg tgagatccgc gaggtgcgcg
     3601 cgcgctacgt cgtcgccgcg gacggcgggc gcaacagcgc cgacatgctg ggcattgaac
     3661 tggagggtcc tcgcgacatg cgcgaggtgg tcaactacca cgtcagcctc gacctctcgt
     3721 cctggtcgga gccggatgcc ctgctcggcc acttcctgca ccccgccggc ggcgcccgca
     3781 ggcgcggcac gatccaggcg ctcgggccca cgcactacaa ccgcgactcg gaggagtggc
     3841 tggtctcggt ggccgcgtgg atgatcgagg gcgaccccga cgacgagagc gcgctcctcg
     3901 aatcgatccg gcggatgctg ggcctggccg acgaccacga gatcacgatg cactcgatga
     3961 ccaggtggaa ctacaacggg ctggtcgcca agcgcttccg agcggggcgg gtcttcctcg
     4021 ccggcgacgc cgcgcacaag catccgccga ccggaggtct cggcctcaac agcggcatcc
     4081 aggacgtgca gaacctgtgc tggaagctgt cggcggtgct gcacgacgag gcccccgagg
     4141 cgctgctgga cagctaccag gccgagcgtc gcccgatcgt cgcctggtac accgcgcact
     4201 cgctcgagaa cgcgaatcgg caccctccga tcgccgaggc gctcggtttc agcgaggacg
     4261 aggcggaggg attccgcaat ctcgaggtct tcgtcgggga gggccccgag agcgacgaga
     4321 tgcgccggcg cgtggcggcc gaggtgcagc ataacgcgca cgactacagc cagctgggcg
     4381 tcgaggcggg ctaccactac tgggccgggg cgttcgtgcc ggacggcacg cccgcgccgg
     4441 gcgacgaatc cgaccccgtg gccttccacc cgacgtcccg tcccgggcat caccttcccc
     4501 acgtgtggct cgcggatgcc gccgggagag cgccccggtc gacgcacgac ctggtccgtc
     4561 cgcacgggct caccctgctc acgtcagcgg atgccgcgcc ggactggcag caggcggtcg
     4621 acgcgctgac cgggacgatg cccgtcaccg tcgtcgccgt cccggatgac gagggctggc
     4681 gcgccgtgcg ccagatcgac gacgacggcg cgctgctgct gcgtcctgat cgcaaggtcg
     4741 cctggcgagc ggcatccgct cccgccgacc gcgcgtcggc actgcgcgac gcgctcgagg
     4801 tggtgctgcg cggcggcgat gctcccgatc acgaccccgc ggaaccgttc ctgaagcgga
     4861 tccgcgccgc cgccagcgtg ctggtgcagt gacaccgaag ggcgcggcgg cggccggccg
     4921 cgcccttcgt acaccgtgtc ggatcggcct gcgggagatt cccgcagcgg gccgatcctc
     4981 gccggatgcc gctctcgtag cgtcgaacag agtcgtacga cacattccga aggagtaccc
     5041 gtgatcatcg atgtccacgg ccacatgacc gcccccgact cgtactacgc gtggaaggcg
     5101 tcgctgctgg ccgcgcgagg atcacacggc ggcaagcccc ccgtgatcag cgacgatgag
     5161 ctgatcgcct cgtaccacgc gccgcacccg agcttcggaa acgtctcgca catggagcac
     5221 atcgacggcg ccggaatcga tctgcagctc atctcgccgc gcccgttcca cgccatgcac
     5281 tccgagcgtc cgacgcgcat cgtcgagtgg ttcacggccg agaccaacaa catcatcaag
     5341 cgcacgaccg agctgttccc cgggcgcttc aagggcatcg cgggcatccc gcagagcccg
     5401 tacctcaccg tcgagcagtg gctgcccgaa ctgcgccgtg cgatcaccga gctcggcttc
     5461 gtcggcgcca tggtcaactc cgacccgtac gagggcaccg aggtgccgcc ggcgatgggc
     5521 gagcggtact ggtatccgct gtacgagacg ctgtgcgagc tggacgttcc gatgctgctg
     5581 cactcggccg gctgcaagtt cccggcacgc gagccgtaca gcctgcactt catccaggag
     5641 gagaccgtct cggtctggag catgatccag gcgaacgtct tcgccgactt ccccgacctc
     5701 aaggtgatcg tcgcccacgg cgggggagcg atcccgtacc agatgggacg attcctgccc
     5761 tctgtcgtgc gcaccggtgt ctcgtacctc gacaagctgc gcaacttcta cttcgattcg
     5821 tgcctctaca cgcaggacag cctcgagctc ctgatgaagg ccgtcggaac cgaccgcgtg
     5881 ctgttcggct cggagaagcc cggcaccgga tcccagatcg acccggagac cgggcgctgg
     5941 ttcgacgaca tccacctgct catcgacgac atcgagtggc tcgacgcgga ccgtcgcgcc
     6001 gacgtgctcg agaacaacgc ccgcagcatc ttccgcctgt aggcaccgaa cgcaattcgg
     6061 gaaaggaccg accatgaccg agatcgtcgc cggattcggc acctcgcacg gaccgcagct
     6121 gaaggccgcc ccgccggagg cctgggagac ccgcggcgtc gccgaccgcc gcagcaaggg
     6181 actgaagttc cgcggcggaa cgtacacctt cgatgagctg cgcgaactgc gcgaggactt
     6241 ctcgcatgag atcacgcacg acgtgatgca gcgccgctgg gacagctgcc agggctcgat
     6301 ggacaaggtc gccgagcaca tcaaggcgca ggacgtggac gtcctggtca tcgtctccag
     6361 cgaccacaag gagacgtacg gcgacgagtg gctcgccccg ttctcggtgt tctggggcga
     6421 cgaggtcgcg cacgtgccgt tcacccagga gcagctcgac gcgatggcgc ccggcctcgc
     6481 cgaggcggcg atgggcgacg tgcccgatca cacgatcatc cgccccacgc atcgcgagct
     6541 gggcaagcac atcatccagt ccacccaggc cgaggacttc gacaccggcg ccaccgaaat
     6601 cgttccggcg ggccgctacg agaaccactc gatcccgcac ggcttcggct tcatctacca
     6661 gcgcttcatg ggcgaggact ccaccgtgcc gatggtgccg atcttcatca acaccttctg
     6721 ggagccgaac ccgccgagcg cgaagcgctg ctacgacttc ggccgcgccg tcgggcgggc
     6781 gatccagtcc ttccccggcg acctgcgggt cggcgtcgtc gcgtcgggcg ggctcagcca
     6841 cttcgtcatc gacgagaagc tcgaccgcga gttcatcgag gccatgaaga accacgacgc
     6901 ggagtacctc tcgcaggtcc ccgccggcga gatgatgtcg ggcgcgtccg agctgcgcaa
     6961 ctggatcgcc gtggccggca tcgccgacga ggtcggcctg caggtcacgg gcgtcgacta
     7021 cgaggcctgc taccgcaccg aggccggcac cggcaacgcg atgggattcg tcacctgggg
     7081 gcgtgcctga gatgacggat gccatcgtcg cggcgctgcg cgcgctggac agctgcgccg
     7141 tctcggatgc cctcgacacc ctcgggctcc ccggtgccgt gacggcgctc acgccgatgt
     7201 ggcccgccgg gcgcacgg
//
LOCUS       DATMSX010000530         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_65777,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000530 DATMSX010000000
VERSION     DATMSX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65777"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VN041_16920"
     CDS             complement(<1..241)
                     /locus_tag="VN041_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30767.1"
                     /translation="MDATTIVLPVVPAEPRRPSLPLMAAVVPVVAGVVLWLVTGSLFA
                     LCFAALGPLMLVASFLDGLRTRGRERRRAQQETDAG"
     gene            complement(300..1094)
                     /locus_tag="VN041_16925"
     CDS             complement(300..1094)
                     /locus_tag="VN041_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWU30768.1"
                     /translation="MPETTTQTHRVSDELQLAWAGVTDRGRRRENNQDSFLARFPLFV
                     VADGMGGHAGGEIASQSTVARLDEMVGDGEVSDAAIQTALHHAVDDIHLHPETTDEGT
                     GTTLTGAYLDHAEDGTPQWTVLNIGDSRVYLKRDDRLVQVTTDHSVVQELIASGKISP
                     EEADGHPYSNVITRAVGASELVPPDYVTVDLRDEDRLVICSDGLTKELTDYGILHFLR
                     EHEDPAEAAEAMLDAALENGGRDNVTLIVVRVLMPHSPSTDADPEE"
     gene            complement(1165..2148)
                     /locus_tag="VN041_16930"
     CDS             complement(1165..2148)
                     /locus_tag="VN041_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWU30769.1"
                     /translation="MGTSQRRVGASGLVVSATGLGCNNFGRAGTVTQTLEGTRPVIDA
                     ALANGVFFFDTADMYGAEPGSSEALMGEALEGRRDRVVLATKFGHARDMGYDFPGGRG
                     SRRYVRFAVEQSLRRLRTDWIDLYQLHLPDPQTPIAETIDALDELVDEGKIRYYGHSN
                     FDGWQIAEAEFTARARSTGRFISAQNHYSLLARASEREVLPAVNRYGLGFFPYFPLKN
                     GLLTGKFTREGGPAGSRIMETRPHIWADAPWDALEAYQAFCDARRITMLQATFGWLLA
                     QPGVASVIAGATTPAQVEANAAASDAWAPSVAELEEIDRLFPLVEDPASAM"
BASE COUNT          349 a          793 c          823 g          350 t
ORIGIN      
        1 agccggcatc cgtctcctgc tgcgcgcgcc gacgctccct gcccctggtg cgcagcccgt
       61 cgaggaacga cgcgaccagc atcagcgggc cgagcgcggc gaagcacagc gcgaacaggg
      121 agcccgtgac cagccagagg acgacacccg cgacgacggg cacgaccgcg gccatcagag
      181 gcagcgaggg gcgtctcggc tcggcgggaa caacgggaag cacgatcgtg gtggcgtcca
      241 tgcgcaccat tgcaccgcac gatcagcgcg ggaaccggcc tgtccacagg ccggaccagt
      301 cattcttctg gatcggcgtc tgtggaggga gagtgcggca tcagcacgcg cacgacgatc
      361 agcgtgacgt tgtcgcgccc accgttctcc agagccgcgt cgagcatggc ctccgccgcc
      421 tcggccgggt cctcgtgctc gcgcaggaaa tgcaggatgc cgtagtccgt gagctccttg
      481 gtcagcccgt cggagcagat gaccagcctg tcctcgtcgc gcaggtcgac cgtcacgtaa
      541 tccggcggca cgagctcact ggcgccgacg gcgcgcgtga tcacgttgct gtacgggtgg
      601 ccgtcggcct cctccgggct gatcttgccc gaggcgatca gctcctgcac aacggagtgg
      661 tcggtcgtga cctgcacaag ccggtcatcg cgcttgaggt agacgcgcga gtcgccgatg
      721 ttcagcacgg tccactgcgg cgtgccgtcc tcggcgtggt ccaggtatgc gccggtgagc
      781 gtggtgccgg tgccctcgtc cgtcgtctcc gggtgcaggt ggatgtcgtc gaccgcgtgg
      841 tgcagagcgg tctggatggc ggcatccgac acctcgccgt caccgaccat ctcgtcgagg
      901 cgggccaccg tgctctggct ggcgatctcg ccacccgcat gcccgcccat gccgtcggcg
      961 accacgaaga gcgggaaccg cgccaggaac gagtcctggt tgttctcacg gcgacgaccg
     1021 cgatcggtca ctccggccca ggcgagctgc agctcatcgc tgacgcggtg cgtctgcgtt
     1081 gtggtctcag gcacgggcgg tctccgtcga gtgggcggat gcaggggcat ccgcgggata
     1141 cagatacatg ctagtggtcg ccgctcacat cgccgacgcg gggtcttcga ccagcgggaa
     1201 cagccgatcg atctcctcca gctcggcgac ggagggcgcc caggcgtcgg aggctgccgc
     1261 attcgcctcg acctgggcgg gcgtggtggc tccggcgatc acgctggcca ctcccggctg
     1321 ggcgagcagc cacccgaacg tcgcctgcag catggtgatc cgccgcgcgt cgcagaacgc
     1381 ctggtaggcc tcgagcgcat cccacggggc atcggcccag atgtgcggac gggtctccat
     1441 gatgcggctg cccgccgggc ccccttcgcg ggtgaatttg ccggtgagca gaccgttctt
     1501 cagggggaag tacgggaaga atcccagccc gtagcgattc accgcgggca gcacctcccg
     1561 ctcggacgcc cgcgcgagca gcgagtagtg gttctgcgcg gagatgaacc ggcccgtgga
     1621 gcgcgcccgg gccgtgaact ccgcctcggc gatctgccat ccgtcgaagt tcgaatgacc
     1681 gtagtagcgg atcttcccct cgtcgacgag ctcgtccagg gcgtcgatcg tctcggcgat
     1741 cggtgtctgc ggatcgggca gatgcagctg gtacaggtcg atccagtccg tgcgcaggcg
     1801 gcgcagggac tgctcgacgg cgaagcgcac ataccggcgt gagcctcggc cacccgggaa
     1861 gtcgtagccc atgtcgcggg cgtgcccgaa cttcgtggcc agcacgacgc gatcgcgacg
     1921 cccctcgagg gcctcgccca tcagcgcttc gctgctgccc ggctcggcgc cgtacatgtc
     1981 ggccgtgtcg aagaagaaca ccccgttcgc gagggccgcg tcgatcacgg gcctggtgcc
     2041 ctccagcgtc tgcgtcacgg tgcccgcgcg cccgaagttg ttgcagccga ggccggttgc
     2101 ggagacgacg aggccggatg cgccgacgcg acgctgcgag gttcccatgc ctccaccgta
     2161 tcgaacgcgc ggacatgccg aggccgcacg caggaacgcc cccgaccgga gtcgggggcg
     2221 ttcgtggcga atcagatcac gccgggggct gcgtcggatc tgccggcggc tcagcggccg
     2281 gcggtgcggg cggagcagga ggcgcgggcg gagtc
//
LOCUS       DATMSX010000531         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_17198,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000531 DATMSX010000000
VERSION     DATMSX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17198"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            779..985
                     /locus_tag="VN041_16935"
     CDS             779..985
                     /locus_tag="VN041_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWU30770.1"
                     /translation="MSTQGTVKWFNSEKGFGFISPDDGGADVFAHYSAIQSSGYRSLE
                     ENQRVEFDVAQGPKGLQAENIRPV"
     gene            complement(1076..2392)
                     /locus_tag="VN041_16940"
     CDS             complement(1076..2392)
                     /locus_tag="VN041_16940"
                     /inference="COORDINATES: protein motif:HMM:NF044815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30771.1"
                     /translation="MSSDIIHLTGGIDVGNGYVKGMILNTDTGVRDRIDLPSAIVATP
                     RQSPKVPLEDAEAFQTMNDAEEDFYNRLDASFSSPLVSDSDRRIFGRSALSVRGSVFS
                     EFEVHDGHASKADQQLSKILVLGVYAAKALRDYVRANDKLPEQELRVKVRTGLALPIS
                     EFVARRYGYAGEFIGAKGSAVHLVTIKNFNTPVSVRIEFEDVQVVAEGASAQYAISEK
                     GEPLANALLTDLRSREPEMLPGVTGADLVAAQNTVGIDVGEGTVNFPVFTNARFNAEA
                     ASTLDMGYGTILENALDRQDEADLRFTSRKQLADFLQTTPSVLQRNRHQRAVQLVEPD
                     IKHLVDQIRRAFSETLSQSGESTEVVYVYGGGSGPIKEELYPELIKAAGDVPVLYLDA
                     RYSRHLNREGLFLAARAAEEAAAAAQVAADGDSSRRGGRKTAKASA"
BASE COUNT          396 a          841 c          878 g          448 t
ORIGIN      
        1 ggacgtagcc gggcagcagc acggcggccg ccacggcgat cgacacgacc agggcgcaca
       61 cgaccagcag caccgttgta ccgcgcgtcc aggtgcgtgc gcgcggcggg cggggaagcg
      121 tcatcggtct cctcggcgga aggtgatgac tgcaagatta gggcctgcgt gcgacgccgg
      181 atgcactccg agagtggagc ggcccggagg gggccggcca cctcgctgtg cagccattcc
      241 ctctccgagc ctgctgatct cccctcaaag tcctctcaca cctgtcgggc ttgaagtcgt
      301 ggacttcgaa ccacccctcc tctagtgtga aagagcatgc gcgtgggaaa tcgatcgcct
      361 ttcgctgaac cccagtcagc gacgggttcc ccagtcaccc ggcgatccac gcatgcactt
      421 cggccctctc gtctgatgca gtccccagac gcacctcgcg aggggtcgaa tccatgatga
      481 caccgtttgg gaaatttgtc ccccaaatgg gggacaaatc atccgaaacc tgtgtatgct
      541 gaaacctgac gcaccctccg gtcgtcttcg gccctcaccg ctccccccgc ggtgggggcc
      601 aaccaattcc cccggaggtc gctgcgaagg ccctgatctc ggccgctgac acgccgatcc
      661 gcatgcaaca tttagatcac cttctgccca ctgctgttgc ctggcgggtc gtctgggata
      721 gcgttagcgg cagtcggtgc atcctgcgtc ggcatcgcaa caagcagaac ggcagtaaat
      781 gagcacccag ggcacggtca agtggttcaa ctcggagaag ggcttcggct tcatctcccc
      841 cgacgacgga ggcgctgacg tgttcgcgca ctactccgca atccagtctt ccggctatcg
      901 ctccctcgag gagaaccagc gcgtcgagtt cgacgtcgcg cagggtccca agggcctgca
      961 ggctgagaac atccgccccg tctgaggcga atgcgaagaa ccccgccgga ctgctcagcg
     1021 cagtgcggcg gggttcttcc gtatccgggg cccgtctccg agccccggac cccggtcagg
     1081 cggatgcctt ggcggtcttc cggcctccgc gacggctgct gtcgccgtcg gcggcgacct
     1141 gagcagccgc agcggcctcc tcggccgcac gggctgcgag gaacaggcct tcgcggttca
     1201 ggtgccgcga gtaccgggca tccaggtaca gcacgggcac gtcgccggcc gccttgatca
     1261 gctcggggta cagctcctcc ttgatcgggc cggagccgcc gccgtagacg tagacgacct
     1321 cggtcgactc acccgactgg ctgagcgtct cgctgaaggc gcgacggatc tggtcgacga
     1381 ggtgcttgat gtcgggctcg accagctgca cggcacgctg gtgccggttg cgctgcagca
     1441 ccgagggcgt ggtctgcagg aagtcggcga gctgcttgcg gctggtgaaa cgcaggtcgg
     1501 cctcgtcctg acggtcgagc gcgttctcga ggatggtgcc gtagcccatg tcgagcgtcg
     1561 acgcggcctc ggcgttaaac cgggcgttcg tgaacaccgg gaagttcacg gtgccctcgc
     1621 ccacgtcgat gccgacggtg ttctgcgcgg cgacgaggtc ggcgccggtc acgccgggga
     1681 gcatctccgg ctcgcgcgag cgcaggtcgg tgagcagcgc gttcgcgagc ggctcgccct
     1741 tctcggagat cgcgtactgc gcggaggcgc cctctgcgac gacctggacg tcctcgaact
     1801 cgatgcgcac gctgacgggc gtgttgaagt tcttgatggt caccaggtgc acggccgagc
     1861 ccttggcgcc gatgaactcg ccggcatagc cgtagcggcg cgcgacgaat tcgctgatcg
     1921 gcagcgccag gccggtgcgc accttgacgc gcagctcctg ctcgggcagc ttgtcgttgg
     1981 cgcgcacgta gtcgcgcagc gccttggccg cgtacacgcc gagcacgagg atcttgctga
     2041 gctgctggtc ggccttgctg gcgtggccgt cgtgcacctc gaactcgctg aacaccgagc
     2101 cgcgcacgct gagcgcggaa cggccgaaga tgcggcggtc gctgtcggag acgagcggcg
     2161 acgagaacga ggcgtcgagg cggttgtaga agtcctcctc ggcgtcgttc atggtctgga
     2221 aggcctcggc gtcctccagc ggcaccttcg gcgactgacg gggcgtggcg acgatcgcgc
     2281 tgggcaggtc gatgcgatcc cgaacacccg tgtcggtgtt gaggatcatg cccttgacgt
     2341 agccgttgcc gacgtcgatg ccgccggtca ggtggatgat gtccgaactc atgggaaggc
     2401 ctttcgttcc gagtcgtcgg ttgaatggtg gtggtgatcg atcacaagcc gaggagatcg
     2461 tcgatggtcg ccgtggcgtc ggtgtcgtcc gagcgcgccg gtcgccgcgc ctgcgtggcg
     2521 ggctcgacgt cgtcgtgcgt ctcggtcttg cgggcggcgg gct
//
LOCUS       DATMSX010000532         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_55718,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000532 DATMSX010000000
VERSION     DATMSX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55718"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN041_16945"
     CDS             complement(<1..218)
                     /locus_tag="VN041_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dioxygenase subunit beta"
                     /protein_id="HWU30772.1"
                     /translation="MSPETTDPRWLDENGHEIDEDHRGLVYDIRTLIDRRRMLGIFGG
                     IAATTLLAACAPTTSASTGSGSGGSAGAP"
     gene            390..827
                     /locus_tag="VN041_16950"
     CDS             390..827
                     /locus_tag="VN041_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWU30773.1"
                     /translation="MDLAYELHDLVRTLDRWAEQILRPEGLSYNQYVALVILGEHPGI
                     TGRILAKAVGVTEAAGSGIVRALLADDLITDQAGPRTGNRRELHLTPRGVDRAQHCGR
                     LLGRSVDDNARTIGIDPAALAATIRALHDEVRTVRTPSGKDRS"
     gene            824..1429
                     /locus_tag="VN041_16955"
     CDS             824..1429
                     /locus_tag="VN041_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HWU30774.1"
                     /translation="MTFFLAAAAAVCGLARLAIFVSLHVIRNEYSPVRHAVSDYAVGS
                     TRRLSSIMTWVTVPFWGLLAAAVATGLNDWADATGIAVALAVLALIFLVLPFVPTDLE
                     GEKASLIGRLHYLLAIAWFAISYACMGNFTRLFSALGPEWLAVTLTVVSWIAAVSLAM
                     SVIVLVVKKLRTWVFGISERVFILSVNLFYLGVAVGLLLVG"
     gene            complement(1430..>2133)
                     /locus_tag="VN041_16960"
     CDS             complement(1430..>2133)
                     /locus_tag="VN041_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWU30775.1"
                     /translation="GGAGLGAVLVVTSVAASTFGMIAAAIAGALLAFLLVYSLSWRGG
                     LSADRFILIGIGVSYGATALTTFVLLRSNPWDTPKIYTWLSGTTYGRLWEQVIPLAIV
                     LAVALPFVIAQRRELDIVAMDEDTPRLVGVRLERARLGLLGAAALLAALSVSAVGVVG
                     FVGLVAPHAARALVGARHSRVIPVSVVLGAVLVGTADAIGRTVLAPAQLPAGLVVALI
                     GAPYFVWLLWRSRDA"
BASE COUNT          341 a          779 c          692 g          321 t
ORIGIN      
        1 ggagcgccgg ccgatccgcc cgatcccgac cccgtggacg cggatgtggt cggcgcgcag
       61 gccgcgagca gcgtcgtcgc cgcgatgccg ccgaagatgc ccagcatccg acggcggtcg
      121 atcagggtgc ggatgtcgta gacgagtccc cgatggtcct cgtcgatctc gtggccgttc
      181 tcgtccagcc agcgggggtc ggtggtctcg gggctcatga tctcgtcctc tcgtcgggcg
      241 ggtcgcccac gttccgaggc aaccagcacg actgatgacc gacgcaggag gatgccaagc
      301 ggctgccgtg agcaacctgt gcgaatcgaa tgaacgactt cgcagatcgg gtcttgcatc
      361 cgactaactt aagtagttaa gttagtgcca tggacctcgc ctacgaactg cacgatctgg
      421 tccgcacact cgacagatgg gcggagcaga tcctccggcc cgagggactc agctacaacc
      481 agtacgtcgc tctcgtcatc ctcggcgagc acccgggcat caccgggcgc atcctcgcga
      541 aggccgtcgg ggtcaccgag gcggcgggca gcgggatcgt ccgtgcgctg ctcgccgacg
      601 acctcatcac cgaccaggcc ggccctcgta ccggtaaccg tcgagagctt catctcaccc
      661 cgcgtggcgt cgacagagcc cagcactgcg gccggctgct ggggcgctcc gtcgacgaca
      721 acgcccgcac catcggcatc gatccggccg ctctggccgc caccatccgc gccctccatg
      781 acgaggtacg caccgtgcgc accccctccg gaaaggaccg ttcatgacct tcttcctcgc
      841 cgcggcagcc gctgtctgcg gactcgcccg cctcgcgatc ttcgtcagcc tccacgtgat
      901 caggaacgag tactcccccg tccggcacgc ggtcagcgac tacgccgtgg ggagcacgag
      961 aagactcagc agcatcatga cctgggtcac cgtgccgttc tgggggctgc tcgcggccgc
     1021 cgtcgccacg ggcctgaacg attgggcgga tgccacgggg atcgccgtcg ccctggccgt
     1081 cctggccctg atcttcctgg tgctgccgtt cgtccccacc gatctcgagg gcgagaaggc
     1141 gtcgctcatc gggcgcctgc actatctgct cgcgatcgcc tggttcgcca tcagctacgc
     1201 gtgcatgggc aacttcaccc gcctcttctc cgccctgggt ccggagtggc tggccgtgac
     1261 gctcacggtc gtctcctgga tcgccgccgt ctccctggcg atgagcgtca tcgtgctcgt
     1321 cgtgaagaag ctccgcacgt gggtgttcgg catctccgag cgggtcttca tcctctcggt
     1381 caacctgttc tacctcggcg tcgccgtcgg actcctcctc gtggggtgat caggcgtcgc
     1441 gcgagcgcca gagcagccag acgaagtacg gggcgccgat cagagccacg accagtccgg
     1501 ccggcagctg cgccggcgcg agcacggtgc gtccgatggc atccgccgtc cccacgagta
     1561 ccgcaccgag cacgaccgac accgggatca cgcggctgtg ccgcgccccc accaatgcgc
     1621 gcgcggcgtg cggtgcgacc aggcccacga agccgaccac gccgaccgcc gagacgctga
     1681 gcgccgccag cagagcagca gcgcccagca gaccgagccg ggcgcgctcg aggcgcactc
     1741 cgaccagccg aggcgtgtcc tcgtccatcg cgacgatgtc gagctcgcga cgctgagcga
     1801 tcacgaacgg cagcgcgacg gccagcacga tcgccagcgg gatcacctgc tcccacagcc
     1861 gcccataggt ggtgcccgac agccaggtgt agatcttcgg ggtgtcccac gggttggagc
     1921 gcagcagcac gaacgtggtc agcgccgtcg ctccgtagga gacgccgatg ccgatcagga
     1981 tgaacctgtc cgcgctgagg ccgcccctcc acgacagcga gtacacgagc aggaaggcca
     2041 gcagcgcgcc ggcgatcgcc gcggcgatca tcccgaaggt ggaggccgcg acgctggtga
     2101 ccaccagcac ggcgccgagc ccggcgccgc ccg
//
LOCUS       DATMSX010000533         1904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_140646,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000533 DATMSX010000000
VERSION     DATMSX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140646"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VN041_16965"
     CDS             <1..1416
                     /locus_tag="VN041_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30776.1"
                     /translation="ELAPEPASTRTETRPRSRRFALALFSVLGVLALVVAALGVVSLT
                     QGPRISSTSVNAAQAIQTSGSRLTLTLNQRVDSLDAKQVSVTPAVPFTVDAAGRSIGI
                     RFTTALNDDTTYSVKVSGVVGAGGGPASDLATTFRTPASRILLLQRAPGGKDDKIFST
                     DLTGERAKPVFEHPRIDDYRVSGNSLVVAVEEKGGSRLMVMDLDGSHQRELKLPGVGF
                     VSSVQLSDRGGYVGYSYSDRDLTETSGRASVLVTQSLNGKGKPRIVEVGGKEASVAEW
                     QFVPDTSSLLFVDFDGALGLQDVRSDGAVKSMGVAASILGVSRGTYTAIVERADGSIM
                     QVDLTDGSETPLPASTPDYGDADAIVPFPGGTVRHVTQRDEGGMPTGQAVVRVDDKGT
                     AKIVTEVSGSDAIVQTCVSPSGQYAAVVVAPDLPSNPYDELLLPLPGTLHTQLIDLDG
                     KRELPTLNGFDISWCAQAPHP"
     gene            1449..>1904
                     /locus_tag="VN041_16970"
     CDS             1449..>1904
                     /locus_tag="VN041_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWU30777.1"
                     /translation="MTHLADRAELVDLPVDVAPRLLGAQLVTVVDGVETRLRITEVEA
                     YHGKGTGAVPDPGSHARMGPTARNATMWGEPGHLYVYLSHGIHSCVNVVCGPEGEAGG
                     VLLRAGEVVGGTDAAAARRGVATPLARMTLRDLARGPGRLGQAVGLRHAL"
BASE COUNT          272 a          688 c          678 g          266 t
ORIGIN      
        1 gagctcgccc ccgagcccgc atccactcgc accgagaccc ggccccgcag ccgtcgcttc
       61 gctctggcgc tgttctcggt gctcggcgtg ctcgccctgg tggtggcggc gctcggcgtc
      121 gtcagcctca cccaggggcc gcggatctcg tcgacgtccg tgaacgcggc acaggcgatc
      181 cagacgtcgg gcagccgcct caccctcacg ctgaaccagc gggtcgactc gctcgatgcg
      241 aagcaggtca gcgtcacgcc cgctgtcccg ttcaccgtcg acgcggcggg gcgcagcatc
      301 ggcatccgct tcaccacggc gctgaacgac gacaccacgt actccgtgaa ggtgtccggc
      361 gtcgtcggcg cgggaggcgg accggcatcc gacctggcca cgacgttccg cactccggca
      421 tcccgcatcc tgctgctgca gcgagcgccc ggcggcaagg acgacaagat cttcagcacc
      481 gacctcaccg gtgagcgcgc caagccggtg ttcgagcacc cgcgcatcga cgactaccgc
      541 gtcagcggga acagcctggt cgtggccgtc gaggagaagg gcggctcgcg gctgatggtc
      601 atggatctgg acggctccca tcagcgcgag ctgaagctcc cgggcgtcgg cttcgtgtcc
      661 tcggtgcagc tctccgaccg cggtggctac gtcggctaca gctactccga ccgcgacctg
      721 accgagacct ccgggcgggc gagcgtgctg gtcacgcagt cgctgaacgg caaggggaag
      781 ccccgcatcg tggaggtggg cggcaaggag gccagtgtcg ccgagtggca gttcgtgccg
      841 gacacctcgt cgctgctgtt cgtcgacttc gacggagccc tgggtctgca ggacgtccgc
      901 tccgacggtg cggtgaagtc gatgggtgtc gccgccagca tcctgggcgt gagccgcggc
      961 acctacaccg ccatcgtcga gcgggccgac gggagcatca tgcaggtcga cctgacggac
     1021 ggctcggaga cgccgctgcc ggcctcgacg ccggactacg gcgacgccga cgcgatcgtg
     1081 ccgttccccg gcggcacggt gcgtcacgtc acccagcgcg acgagggcgg catgcccacc
     1141 ggccaggccg tcgtgcgggt cgacgacaag ggcaccgcga agatcgtcac cgaggtcagc
     1201 ggctcggatg ccatcgtgca gacgtgcgtg tcgcccagcg ggcagtacgc ggctgtggtg
     1261 gtcgcgcccg acctgccgag caacccctac gacgagctgc tgctgccgct gcccggcacg
     1321 ctgcacacgc agctgatcga cctcgacggc aagcgggaac tgcccacgct gaacggcttc
     1381 gacatctcct ggtgcgcgca ggcgcctcac ccgtgagcgc tcgcagcgcc gaccgccgag
     1441 gaggaggcat gacgcatctc gccgatcgcg ccgagctggt cgacctgcca gtggacgtgg
     1501 ccccgcggct gctcggcgcg cagctggtca ccgtcgtcga cggcgtcgag acccggctgc
     1561 ggatcaccga ggtcgaggcg taccacggca agggcaccgg cgccgtgccc gatcccggct
     1621 cgcacgcgcg gatgggaccg accgcacgca atgccacgat gtggggtgag cccggtcacc
     1681 tctacgtgta cctcagccat ggcatccact cctgcgtcaa cgtcgtgtgc ggcccggagg
     1741 gcgaggcggg cggtgtgctg ctgcgcgccg gggaggtggt cggagggacg gatgccgcgg
     1801 ctgcgcgccg cggcgtcgcg accccgctcg cccgcatgac gctgcgcgat ctcgcccgcg
     1861 gtccggggcg gctgggccag gccgtcggcc tgcggcatgc gctg
//
LOCUS       DATMSX010000534         8438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_122160,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000534 DATMSX010000000
VERSION     DATMSX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122160"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3122)
                     /locus_tag="VN041_16975"
     CDS             complement(<1..3122)
                     /locus_tag="VN041_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWU30778.1"
                     /translation="MSNLSVLSLRNRALIALITIVSAVFGGLALTNLKQELIPSLELP
                     ALMVMTTYPGASPEVVENDVSTPIEDAIQGVPGLESTTATSTTNASIVQATFTYGTNL
                     ATAEQKMQQAINRIGQQLPKDVTPQVLSVSIDDFPVIQIAVTGFSDAEAAQNDLQTTV
                     IPDLEDVKGVNAAQIVGGVGQRITITPDAAKLAEARQSTQAISQALQQNGVLLPGGDI
                     TQGDETLTVQTGSKITSASELASLPLVGTTLTIGDVATVEQTTDPVTSISRVDGKDAL
                     SIAVTKLPSANTVDVSKGVLSKLDDLKKTFPDTTFTVVFDQAPFIEQSIETLATEGLL
                     GLAFAVIVILIFLLSIRSTLVTAISIPTSVLITFIGLQAFGYSLNVLTLGALTIAIGR
                     VVDDSIVVIENIKRHYVQGADKGDAIRLAVREVVAAITASTITTVAVFLPIVFVGDMV
                     GELFRPFAMTVTIAMVASLLVALTIVPVLAYWFLKPGKPLLDAAGAQIDPEHPDAPPS
                     RLQRAYRPILGWTLKHSATTVIIAVLVLAGTLAAAPLMKINFLSDSGQNTMTVTQDVG
                     STASLEAKSDAAKKVEKALEDVDGITHVQASIGSSGSALRDAFSGGAGITYSIQTSDS
                     ADQEKLRTTVQKRIDELKDAGDVKVGASSGGFGSSDITISVTAPTADVLQEATDALTK
                     DLKGRDGVGEVTDNLAASLPYIAVVVDRAKAAEHGLSEVAVGSIVSNTMRPEQLGTVE
                     VDGTGLTVYLVAPEPPTSEDALKELAIPTPTGIVKLSDIAQVEQSNGPTAITTEKGRR
                     TATITVPPASDDLAVATRSVTASLGHVKLPEGARAEIGGVAQQQADSFSQLGLAMLAA
                     ILIVYVVMVATFKSLRQPLLLLVSVPFAATGAILLQIATGVPLGVASLIGVLMLIGIV
                     VTNAIVLVDLVNQYREKGLATADAVMAGGEKRLRPILMTALATIFALTPMALGITGHG
                     GFISQPLAIVVIGGLVSSTVLTLIVLPTLYNLVEGAKERRAARRAARGGGASGPDGSG
                     PGGSGPAGPE"
     gene            complement(3133..3714)
                     /locus_tag="VN041_16980"
     CDS             complement(3133..3714)
                     /locus_tag="VN041_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWU30779.1"
                     /translation="MSDAVTRRRRDPETRRREIVAAAAELIVEVGVGALTHRMIAARA
                     GVPLGATTQYFATLDELRSEALRHLVSDVDARMEGIRSVFAERGATPAVIAELIVQGL
                     ADARAMEADRAVVTAAVNDPQLRELARRWSAQMAEFLTGDYGPERATAAAIFIDGVLW
                     HSRINDEPLSGTFIESALATILGESIRPSSVSD"
     gene            complement(3784..4143)
                     /locus_tag="VN041_16985"
     CDS             complement(3784..4143)
                     /locus_tag="VN041_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HWU30780.1"
                     /translation="MSPLVFLLAAACGGLGAATRYLVDLGIARLTGTRFPWGVMVINI
                     TGSFALGLVIGGLPGAAFVLGTGFLGGYTTFSTAMIDTVALWRDGERRASALYAGGML
                     LAGLAAATLGLALGHAL"
     gene            complement(4140..4520)
                     /locus_tag="VN041_16990"
     CDS             complement(4140..4520)
                     /locus_tag="VN041_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HWU30781.1"
                     /translation="MASPMSVSTVLRRAALVALGGMLGTAARMGLGMLIPDAALGVLA
                     ANIVGALALGVLTARLPAGDLRVLLGTGVLGGFTTYSSFMVDSVQLWDASPLLAVGYI
                     VVSLVGGLAAAALGLLLGRKRAAS"
     gene            4745..6913
                     /locus_tag="VN041_16995"
     CDS             4745..6913
                     /locus_tag="VN041_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30782.1"
                     /translation="MRDHRTARRSIRWRRLTPALAIGAIGAVALAGAARADTTPPPPQ
                     YVAAGTTTMKGGLTSDIAGSAFVGKDGRFHWTNALTAYSTNRGSAWARTFTNDDMSAV
                     SAGMGTTTERLQAPAYYGDPGTICYQLDKHPVFPIPSPQQDDHCDVIGVWVDPQGVWH
                     ALLNDEYQFDPWQTKGANATIDEKISTGHHNNRILLATSDDEGATWQYAGPALTSAWD
                     DDQVINSTASPGQSYPFGNSGCRLFIDYSTGYFYITYNVKIYKKPASSTLASWTEMAR
                     APISGGMASGTWQKWHNGSWSQPGIGGIDGNVNSIGGLNVFYDPATDLIAWQGSGTNA
                     DASYEAAKVAADHLIRFSDASGNSYTANTLAHTIVNDATGQSAPDRTVSYHDAITGQD
                     MTLKAVDDRYEGGVKVVTGGIYVTQTDPATGVSQTVDLITNSAYYKDPDTGHLYLPAQ
                     NNESAISYNAFSGLYRIVGYDGNVYETADLGDPNSWKAVGLMPAGSNGGYLTSLDNGS
                     LTNQNVTGRSFLTISDLTAKVVDIAPAPGAPGASVSTEYTPRDTAGNAVAAGSSYVLR
                     VGQRAVVTDRGPGMDGGQGAATKATAWRLEPVADQSAKGENSGFYRLVDATTGKVLRV
                     SGTGAAAPRAFDAVVTAGGRQADAVPLTSSSLGTPGGSDQWYLQRVAVNGTSLDGSTT
                     YRLVNRNSGLALQYVDDRMRLEQQKPGDAAQYVMLTPSAG"
     gene            7058..7774
                     /locus_tag="VN041_17000"
     CDS             7058..7774
                     /locus_tag="VN041_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HWU30783.1"
                     /translation="MLTRRIFGWLRLLAAAVCIVSLTHRMLWGMGSQTLASDNFFAYL
                     TIQSNVAFALVTILAGVIALRRAEDPVWLTSARALVLSWTVTAGLAFSIIVWQAGVRG
                     IPITVPWSDVVLHYLLPGCAVIAWLLGPGRHAASWRVVPFVLAYPVVWGVFTIVRGRI
                     IGWYPYYFLDPRQVSGLPEMSVSCLAALAIFALVAAALVLLSHVRVRLPPWGVISSRP
                     PEWARRRRVPLWRRPGRRGR"
     gene            complement(7698..8312)
                     /locus_tag="VN041_17005"
                     /pseudo
     CDS             complement(7698..8312)
                     /locus_tag="VN041_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(8363..8428)
                     /locus_tag="VN041_17010"
                     /pseudo
     CDS             complement(8363..8428)
                     /locus_tag="VN041_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1357 a         2872 c         2945 g         1264 t
ORIGIN      
        1 ctctcggggc cggcaggacc cgagccaccg ggaccggatc cgtccggtcc ggaggctccg
       61 ccgccgcgtg cggcccgccg tgcagcacgc cgctccttcg caccctcgac caggttgtag
      121 agcgtcggca gcacgatcag cgtcagcacc gtcgacgaca ccaggccgcc gatgaccacg
      181 atcgcgagcg gctgggagat gaacccgcca tgtccggtga tgccgagggc catcggcgtg
      241 agcgcgaaga tcgtggcgag cgccgtcatc aggatcggcc gcagacgctt ctcaccgccg
      301 gccatcacgg catccgcggt cgcgaggccc ttctcacggt actggttgac gaggtcgacg
      361 agcacgatcg cgttcgtgac cacgatgccg atcagcatca gcacgccgat cagcgaggcg
      421 acgcccagcg gcacgcccgt ggcgatctgc agcaggatcg caccggtcgc cgcgaacggc
      481 accgacacga gcagcagcag cggctgtcgc agcgacttga aggtcgcgac catcaccacg
      541 tagacgatca ggatcgcggc gagcatcgcg aggcccagct gcgagaacga gtcggcctgc
      601 tgctgcgcga cgccgccgat ctcggcgcgg gcgccctcgg gcagcttcac gtgaccgagc
      661 gaggcggtca ccgagcgcgt ggcgacggcc aggtcgtcgg aggccggcgg caccgtgatc
      721 gtggcggtgc gacgcccctt ctcggtggtg atcgcggtgg gcccgttgct ctgctcgacc
      781 tgggcgatgt ccgacagctt cacgatgccg gtcggggtcg ggatcgccag ctccttcagg
      841 gcgtcctccg atgtcggagg ttcgggagcg acgaggtaca cggtcaggcc ggtgccgtcg
      901 acctccaccg tcccgagctg ctccgggcgc atcgtgttgg acacgatcga gcccaccgcg
      961 acctcggaga ggccgtgctc ggcggccttc gcccggtcga cgaccaccgc gatgtacggc
     1021 agcgacgccg cgaggttgtc ggtgacctcg ccgacgccgt cgcggccctt gaggtccttc
     1081 gtcagggcgt ccgtcgcctc ctgcagcaca tcggccgtgg gagccgtgac gctgatggtg
     1141 atgtcgctgg agccgaagcc gcccgacgac gccccgacct tgacgtcacc ggcgtccttc
     1201 agctcgtcga tcctcttctg cacggtggtg cgcagcttct cctgatcggc cgaatcgctg
     1261 gtctggatgg agtaggtgat gcccgcgccg ccggagaagg cgtcgcgcag cgccgagccg
     1321 ctggacccga tcgacgcctg cacgtgcgtg atgccgtcga cgtcctccag cgccttctcg
     1381 accttcttcg cggcatccga cttcgcctcg aggcttgcgg tgctgccgac gtcctgggtg
     1441 acggtcatgg tgttctggcc ggagtcgctg aggaagttga tcttcatcag cggggcggcg
     1501 gccagggtac cggcgagcac cagcacggcg atgatgacgg tggtcgccga gtgcttcagc
     1561 gtccagccca ggatcggccg gtaggcgcgc tgcagacggc tgggcggagc atccggatgc
     1621 tccggatcga tctgcgcccc cgcggcgtcc agcagcggct tgccgggctt gaggaaccag
     1681 tacgccagca ccggcacgat cgtcagcgcg accagcagag aggcgaccat cgcgatcgtc
     1741 acggtcatcg cgaagggccg gaacagctcg ccgaccatgt cgccgacgaa cacgatcggc
     1801 aggaacaccg cgacggtcgt gatcgtggac gccgtgatgg ccgcaaccac ctcgcgcacc
     1861 gccagccgga tcgcatcgcc cttgtcggcg ccctgcacgt agtgacgctt gatgttctcg
     1921 atcaccacga tggagtcgtc gaccacgcgc ccgatcgcga tggtgagcgc gccgagcgtc
     1981 agtacgttga gcgagtagcc gaacgcctgc agtccgatga aggtgatcag cacgctggtc
     2041 gggatcgaga tcgcggtgac cagtgtggag cggatcgaca gcaggaagat caggatgacg
     2101 atcactgcga acgccaggcc cagcaagccc tcggtggcga gcgtctcgat ggactgctcg
     2161 atgaacggcg cctgatcgaa cacgacggtg aaggtcgtgt cggggaaggt cttcttcagg
     2221 tcgtcgagct tgctcagcac gcccttggac acgtcgaccg tgttggcgga cggaagcttg
     2281 gtgacggcga tcgacagggc gtccttgccg tccacgcgcg agatcgaggt gaccgggtcg
     2341 gtggtctgct ccacggtcgc gacgtcgccg atggtcagtg tcgtgccgac cagcggcagg
     2401 gatgccagct cgctcgcgct cgtgatcttc gacccggtct gcacggtgag cgtctcgtca
     2461 ccctgggtga tgtcgccgcc gggcagcagc acgccgttct gctgcagcgc ctggctgatc
     2521 gcctgcgtgc tctgacgcgc ctcggccagc ttcgccgcgt cgggcgtgat cgtgatgcgc
     2581 tgcccgacgc cgccgacgat ctgggcggcg ttgacgccct tgacgtcctc gaggtccggg
     2641 atgacggtgg tctggaggtc gttctgcgcg gcctcggcat cgctgaagcc ggtgacggcg
     2701 atctggatca ccgggaagtc gtcgatcgag acgctgagca cctgtggcgt gacgtccttg
     2761 ggcagctgct ggccgatgcg gttgatggcc tgctgcatct tctgctcggc ggtcgccagg
     2821 ttggtgccat aggtgaaggt cgcctgcacg atcgaggcgt tggtggtgct cgtcgcggtc
     2881 gtcgactcga gcccaggcac gccctggatg gcatcctcga tcggcgtgga gacgtcgttc
     2941 tcgacgacct cgggcgaggc gccggggtac gtggtcatga ccatcagcgc cggcagctcg
     3001 agcgagggga tgagctcctg cttgaggttg gtcagcgcca gtccgccgaa aacggcggag
     3061 acgatcgtga tcagggcgat gagcgcgcgg ttgcgcaggc tgaggacgga gagattcgac
     3121 acgatgctcg tttcagtcgc tcacggacga aggacggatg ctctcgccga ggatggtggc
     3181 gagagcggat tcgatgaagg tgccgctgag cggctcgtcg ttgatgcggg agtgccacag
     3241 caccccgtcg atgaagatgg cggccgcggt cgcgcgctcg ggaccgtagt cgccggtgag
     3301 gaactccgcc atctgcgccg accagcgccg cgcgagctcg cgcagctgcg ggtcgttgac
     3361 cgcggcggtg accacggcac ggtcggcttc catggcgcgg gcgtcggcga gcccctgcac
     3421 gatgagctcg gcgatgacgg cgggagtggc accgcgctcg gcgaagacgg atcggatgcc
     3481 ctccatcctg gcgtccacat cggacaccag gtgtcgcagt gcctccgacc gcagctcgtc
     3541 gagcgtcgcg aagtactgcg tggtcgcgcc gaggggcacg cccgcgcgcg cggcgatcat
     3601 gcggtgggtg agcgcgccga ccccgacctc gacgatcagc tcggccgcgg cagcgacgat
     3661 ctcgcggcgg cgggtctccg gatcgcgcct gcggcgcgtg acagcatccg acatcccgac
     3721 ccccttatgt acatctgtac attattggcc cttggaaccg gctgagagcg ggactggagc
     3781 cgatcagagc gcgtgaccga gcgcgaggcc cagagtcgcc gcggcgagtc cggccagcag
     3841 catcccgccc gcgtagagag cggatgcccg gcgctcaccg tcgcgccaca gcgcgacggt
     3901 gtcgatcatc gccgtgctga aagtggtgta cccgccgagg aagccggtgc ccagcacgaa
     3961 ggcggctccc ggcagcccgc cgatcaccag gccgagggcg aacgacccag tgatgttgat
     4021 caccatcacg ccccacggga agcgcgtgcc ggtcagccgt gcgatgccga gatcgacgag
     4081 gtaacgcgtc gcggccccga gtccgccgca cgcggcggcc agcaggaaca ccagcgggct
     4141 catgacgccg cccgcttccg cccgagcagc agtccgagag cggcggccgc gagcccgccg
     4201 accaggctga cgacgatgta cccgacggcg agcagcggag atgcatccca cagctgcacg
     4261 ctgtcgacca tgaacgagct gtaggtcgtg aagccgccga gtacgcccgt gccgaggagc
     4321 acccgcaggt ccccggcggg caggcgggcc gtcaggacac ccagggccag ggcgccgacg
     4381 atgttcgcgg ccagcacgcc cagcgcggca tccgggatga gcatcccgag acccatgcgc
     4441 gcggcggtgc ccagcatccc gccgagcgcg accagtgccg cccgccggag gacggtggag
     4501 acggacatcg gggaggccac cgtccaactc tagccagagc gggttcccgt cgtccggcgc
     4561 gtgtgcgcgg ggcatgcggc tggcgcacgc cggccaccat tggccaaaag ttcagtaatc
     4621 ggtttctaga aacgagttac cgttgacagc ctcagaatct ctcggatacg ttcacccaac
     4681 agtaagcgat ttctatcgcc tgcacccagc actccgcttc gaagacgaac aggaaggcac
     4741 gacaatgaga gaccatcgca ccgcccgccg cagcatccgc tggcgacgcc tcaccccggc
     4801 actggccatc ggagcgatcg gcgcggtcgc actcgcaggt gccgcccgcg cggacacgac
     4861 tccccctccc ccgcagtacg tcgcagcggg gacgaccacg atgaagggcg ggctgaccag
     4921 cgacatcgcg ggctccgcct tcgtcggcaa ggacggccgg ttccactgga ccaacgcact
     4981 cacggcgtac agcacgaacc ggggcagcgc ctgggcgcgc accttcacga acgacgacat
     5041 gagtgccgtc tcggcgggca tgggcaccac cacggagcgg ctgcaggcgc ccgcgtacta
     5101 cggagacccc ggcacgatct gctatcagct cgacaagcac cccgtcttcc cgatcccgtc
     5161 accgcagcag gacgatcact gcgacgtgat cggcgtgtgg gtcgacccgc agggcgtctg
     5221 gcatgcgctg ctgaatgacg agtaccagtt cgatccgtgg cagaccaagg gtgcgaacgc
     5281 gacgatcgac gagaagatct cgaccggtca ccacaacaac cgcatcctgc tcgccacgtc
     5341 ggatgacgag ggcgcgacct ggcagtacgc gggtcccgcg ctcacctcgg cctgggatga
     5401 cgaccaggtc atcaacagca cggcgtcgcc cgggcagtcg tacccgttcg gcaactccgg
     5461 ctgccgcctg ttcatcgact actccaccgg ctacttctac atcacgtaca acgtgaagat
     5521 ctacaagaag ccggcctcgt cgaccctggc cagctggacc gagatggcgc gcgcgccgat
     5581 cagcggcggc atggccagcg gcacgtggca gaagtggcac aacggatcgt ggtcgcagcc
     5641 gggcatcggc gggatcgacg gcaacgtcaa ctccatcgga ggactgaacg tcttctacga
     5701 ccccgccacc gatctgatcg cctggcaggg atcggggacg aatgcggacg cctcgtacga
     5761 ggccgcgaag gtcgcagcgg accacctcat ccggttctcg gatgcctcgg ggaacagcta
     5821 cacggccaac acgctggcgc acacgatcgt gaacgacgcg accgggcagt ccgcccccga
     5881 tcgcaccgtg tcgtaccacg atgcgatcac ggggcaggac atgaccctca aggccgtgga
     5941 cgacaggtac gagggcggcg tcaaggtcgt caccggcggc atctacgtga cgcagaccga
     6001 tccggcgacc ggcgtctcgc agaccgtcga tctcatcacg aacagcgcct actacaagga
     6061 cccggacacc ggccacctgt acctgcccgc gcagaacaac gaatcggcca tctcgtacaa
     6121 cgcgttctcc ggcctgtacc ggatcgtcgg ctacgacggc aacgtgtacg agaccgccga
     6181 tctgggagac ccgaactcgt ggaaggccgt cgggctcatg cccgccggct ccaacggcgg
     6241 ttacctcacc tcgctcgaca acggcagcct gaccaaccag aacgtcaccg gccgctcgtt
     6301 cctgacgatc tccgacctga ccgccaaggt cgtggacatc gctccggcgc ccggcgcccc
     6361 tggcgcctcg gtgagcacgg agtacacccc gcgggacacg gcggggaacg cggtcgccgc
     6421 gggctcctcg tacgtgctgc gagtgggaca gcgggccgtg gtcacggatc ggggccccgg
     6481 catggacggc ggccagggtg cggccaccaa ggccacggcc tggaggctcg agcccgtcgc
     6541 cgatcagagc gcgaaggggg agaacagcgg cttctaccga ctcgtggacg cgacgaccgg
     6601 caaggtcctg cgcgtctcgg gcaccggtgc cgccgcgccg cgtgcattcg acgccgtggt
     6661 gaccgccggc ggccggcagg cggatgccgt gcccctgacc tcgtcgagcc tgggcacccc
     6721 cggtggcagt gaccagtggt acctgcagcg cgtcgccgtg aacggcacct cgctggacgg
     6781 ctcgaccacg taccgtctgg tcaaccgcaa cagcgggctg gcgctgcagt acgtggacga
     6841 ccgcatgcgg ctcgagcagc agaagcccgg agatgcggcc cagtacgtga tgctgacacc
     6901 gtccgccggc tgagcggcct gatccgacgc gacacacgcg cgggagtgcg gcgtcctgca
     6961 cagcggggcg ccgcacttct gcgcgttacg tagactcggg ggacgactga gccgctccgc
     7021 ccgagggagc ggaagccgcc aggaggacga cgcccgcgtg ctgacgagac ggatcttcgg
     7081 atggctgcgt ctgctcgccg cagccgtgtg catcgtgtcc ctcacgcatc ggatgctgtg
     7141 gggcatgggc tcgcagaccc tggccagcga caacttcttc gcgtacctga cgatccagtc
     7201 gaacgtggcg ttcgccctgg tgacgatcct cgcgggcgtc atcgccctgc ggcgggcgga
     7261 ggatccggtg tggctgacgt cggcccgagc gctggtgctg agctggacgg tgaccgccgg
     7321 cctggcgttc tcgatcatcg tgtggcaggc aggcgtgcgg ggcatcccga tcacggttcc
     7381 gtggtcggat gtggtgctgc actacctgct gcccgggtgc gcggtgatcg cctggctgct
     7441 cggccccggg cggcacgcgg cctcgtggcg agtggtgccc ttcgtgctgg cgtacccggt
     7501 ggtctgggga gtgttcacga tcgtgcgggg ccggatcatc ggctggtacc cgtactactt
     7561 cctcgacccg cggcaggtgt cgggccttcc ggagatgtcc gtgtcgtgcc ttgccgcgct
     7621 ggcgatcttc gctctcgtcg cggccgcgct cgtgctgctc agccacgtgc gcgtgcggct
     7681 gcccccgtgg ggcgtgatca gctcgcgtcc gccggagtgg gcgcgaagaa gacgagtgcc
     7741 gctctggcgg cgacctgggc gtcgcggtcg ctgagcgccg cgaagcgcgc agcatccgct
     7801 ccgcccacga gccccacgag caccggctcg ccggtggccg gcatgaggtt gatccaggtg
     7861 cggatcaggc cgtcgccgcc gacgacgtgc cagatcgtct gctcggcatc ccagaacggc
     7921 tcctcgaacc gcatccacgc cgtctccacg aaaccggatg ccagggcggc gatcgctccg
     7981 cgatgcggct tcggcagcgc gggggcgaac tcgatgccct gccgctgcag cactcccagg
     8041 ggtgccgtga tgatgacgcg gtcgaaggac agcgcctcgc ccgtgcccag ccgcaggctc
     8101 actccgctgt cgtcgtaggc gacgcgcacg accggcgatg ccagcgtgac ctgctgatct
     8161 tcgaggtgct tgtcgaagaa cgaaccgagg tcctcggcca tgccggtgag cgtgtcgggt
     8221 gcgaacgccg gcgggtacca gctcgacgcc ttctcggggt cggcgcccgt ggtggcggag
     8281 agccaggcga gggacgccgc gagcgcgggg tcgtcgaggt cggcgccgtt ctgcgtgagg
     8341 gcctcggcga gcgcttctgg atttcgaaag cgacttcatt ggcaccgccg tgcttcgggc
     8401 cgcgcagtgc accgatcgcg ccggtgatgg ccgaatag
//
LOCUS       DATMSX010000535         7128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_112319,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000535 DATMSX010000000
VERSION     DATMSX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112319"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="VN041_17015"
     CDS             complement(<1..1749)
                     /locus_tag="VN041_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWU30784.1"
                     /translation="MQTFTVDNWRFALADHREAWMKGFDDASWRAVAVPHDWSIEQDF
                     APEHSSGTGYLPGGIGWYRAHVPLSSLALASDSRVELAFGGVYKNADVWVNGYHLGGR
                     PNGHVPFSFELTELLSYAPDDDLVISVRVDRTEISDSRWYNGAGLVRPVALEVHGPVR
                     VLSTAFSTAEADAAEATVHVEQRIDGALDEPRTVQVRHELRSLASDRVHAVETSVDLP
                     AGGTADVRSELRIADPDLWSETAPELYRLTTTLSWDGASVSCQETVGIRTFAFDPDHG
                     FSINGEPRILKGVCLHEDAGCFGVAVPAAVWLRRLLKLKEMGCNAVRMAHNPHASELH
                     ALCDALGLYVIDEAFDEWESPKNKWWQGHNVYPPRHEGYATHFPDWHERDLRAQIEAH
                     RNHPSIIAWSIGNEIDYPNDPYASPLFEEMTGNNDAAKPAEERRYDPNRPDIRRLTTI
                     ARRLGAIVRDADPTRPVTLAAAFPELSSRTGLLDDLDVVGYNYKEHLYQEDHLRFPEK
                     PFLGSENGHGYAQWRAVADNAFIAGQFLWTGIDYLGEAHGWPVHGSGAGVLTMAGFAK
                     PGWHLRRSWWAGEPVAH"
     gene            complement(1754..2395)
                     /locus_tag="VN041_17020"
     CDS             complement(1754..2395)
                     /locus_tag="VN041_17020"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HWU30785.1"
                     /translation="MGSIFAADSPLMRTLTRIADVMILNILFIVTALPVITIGAALTA
                     LNFTAMRIATGTDRTITGDYLRSFRGNLRQATAIWGILLGLAAVLVAWYGVISALVTD
                     PVVQFVLYAGWYIIVFLVVVMTLWVFPYLAKFEGSIRDTLRNARLLSWRHPLTTLFAL
                     TVIVLCATVTVLSPQATGYGLLWLLIGFGGIALVDGTLFARIFDRYIHSSEEG"
     gene            complement(2463..4022)
                     /locus_tag="VN041_17025"
     CDS             complement(2463..4022)
                     /locus_tag="VN041_17025"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30786.1"
                     /translation="MKRRILKAGAVIATLGIGMTMLAGCTASGEDDPSKPTKITMLVL
                     GDKPTNGRLEAMLKKLNANLKKQANATLDLFYVEWADWQTQYNVQLLSGDDKVDLITT
                     ATDWLFGWENAEKGAFLPLSEDMLKANAPKTWAQVEKSGDWDLTKLDGKIQFIPEDDY
                     TQYTNHGFFYRGDWAKEAGFADGTISTFEDFTKYFQWIKKNKPEAYPWDVASTGGNDA
                     VLTGYLQGHTDQMPLQGVSAGNYAPFTTSESDPGEVTSWYMEGDELEKAAELAKEWNR
                     LGVWREDALNYDGDTREEFYSGLSGTDQHHTQTFIGQIWNNMTTKQPGSEPKMFYWGQ
                     ENGNLYKDIKTHGAMAVSANSKHPEKALQVYDLIRNDKADYDLLNFGIEGTDYVMKDG
                     KLGYPEGYDASKDSLGSNFWAGRRDEFEPAKVTDDPDRDKIYGALDKVARDYPYSTLI
                     IDKSSIDPTLAAMGAVLSEYIPQLNYGKFDDPAKAIKEMRQKLKDAGYEDVRASLQKD
                     VDAWAKDHPLK"
     gene            complement(4117..5118)
                     /locus_tag="VN041_17030"
     CDS             complement(4117..5118)
                     /locus_tag="VN041_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30787.1"
                     /translation="MPSSDLTMNPPVTDTGATKTLLLPTRDKSLFARSPRPRSIRLSA
                     SDRWLRGISYVWITLFALFCLLPFVLIVSASFSSEAAIRKDGFGLLPKGFSLAAYELI
                     FQAPRQIIGAYVVTVTMTVLGTAIGLFIIAMTGFALYRQDFPFRNHISFFIYFTTLFS
                     AGLAPTFLWITQGLHLRGSYLAVFLQLLMTPWLIILMKNFMRTVPYEIVESGKIDGAG
                     DFRIFAQLTLPMMKPALATIGLFLALAYWNEWYLSSLYLGSTVEFKPLQYYLYNIINT
                     ANALRNSVAGANVSISDLPSNTLKMASAVVATGPILLFYPFVQKYFVSGITIGAVKG"
     gene            complement(5105..5983)
                     /locus_tag="VN041_17035"
     CDS             complement(5105..5983)
                     /locus_tag="VN041_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWU30788.1"
                     /translation="MLILMCVPAIAFFLVFNYAPLPGIWIAFTDFNYRDGIFGSPFVG
                     FKNFEFLVKSGMLWDLTRNTILYNVAFIVLGNVLQIAIAIMFNEVRLKFFKKITQGVM
                     FLPYFISVVLIGVIAFNLLNYDSGALNTLIKQTGGDPIKIYSMAGLWPLIIIIVHLWQ
                     STGYGSIVYFAAIMGIDKGLFEAAEIDGASAWQRIRYVILPSLKPTFIILFLFSLGGI
                     MSGNFGLFWNLVGNNAALFSTTDIIETSVYRMVMSQNNFTTSTAVGLYQSLFGFAIVM
                     TANWLVRRMNKDYALF"
     gene            complement(6183..>7128)
                     /locus_tag="VN041_17040"
     CDS             complement(6183..>7128)
                     /locus_tag="VN041_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HWU30789.1"
                     /translation="SAALAGCAPAVAAPPQLSGDVFVHDPAYVHTDDGDFVYSTGDGR
                     IADGNIQIRRSDDGADWEYAGEVWQQKPAWLEEAVPGVDNLWAPELYRHDGTWYLYYA
                     ASTFGSNRSVIALATTTTLDPDDPDYEWVDRGEVIGSAGTDFNAIDAGIVEDADGTPW
                     MAFGSFSSGIRMVELEWPSGLRADDAEPLRLADRGLVENAIEAPYIVPHHGWFYLFAS
                     RGFCCKGVDSTYEIIVGRSKDVRGPYLDADGGRLLEDGGTVVLTTDGDRIGPGGQSVS
                     GGMLAFHWYDGAAEGVPTLGILPLEWTDGWPAVHPSAG"
BASE COUNT         1152 a         2246 c         2480 g         1250 t
ORIGIN      
        1 gtgcgcgacc ggctcgcccg cccaccagct gcgtcggaga tgccagcccg gcttggcgaa
       61 gccggccatc gtgagcacac cggcgccgga gccgtgcacg ggccagccgt gcgcctcgcc
      121 caggtagtcg atcccggtcc agaggaactg ccccgcgatg aacgcgttgt cggccacggc
      181 acgccactgc gcgtagccgt gtccgttctc gctgccgagg aacggcttct ccgggaagcg
      241 cagatggtcc tcctggtaga ggtgctcctt gtagttgtag ccgaccacgt cgaggtcgtc
      301 gagcagcccg gtgcggctgc tcagctcggg gaacgccgcg gcgagcgtca ccggccgggt
      361 cggatcggcg tcccgcacga tcgcgccgag tcgccgcgcg atcgtggtca gccggcggat
      421 gtcaggacgg ttcgggtcgt atctgcgctc ctcggcaggc ttcgcggcgt cgttgttgcc
      481 cgtcatctcc tcgaacagag ggctggcgta gggatcgttg gggtagtcga tctcgttgcc
      541 gatgctccag gcgatgatcg acggatggtt gcggtgcgcc tcgatctgcg cgcggagatc
      601 ccgctcgtgc cagtcgggga agtgcgtggc gtatccctca tgccggggcg ggtagacgtt
      661 gtgcccctgc caccacttgt tcttcgggct ctcccactcg tcgaacgcct cgtcgatgac
      721 gtacaggccc agcgcgtcgc acagcgcgtg cagttcggag gcgtgcgggt tgtgcgccat
      781 gcgcacggcg ttgcagccca tctccttgag cttcagcaga cggcgcagcc agacggcggc
      841 gggcacggcg acgccgaagc atccggcgtc ctcgtgcagg cagacgccct tcaggatgcg
      901 gggctcgccg ttgatcgaga agccgtgatc gggatcgaag gcgaaggtgc ggatgccgac
      961 ggtctcctgg caggacaccg aggcaccgtc ccagctcagc gtggtggtca gccggtacag
     1021 ctcaggcgcg gtctccgacc acaggtccgg atctgcgatc cgcagctcgg agcggacatc
     1081 ggccgtcccg cccgcgggaa gatcgaccga ggtctcgacg gcgtgcacgc ggtcggatgc
     1141 cagcgagcgc agctcatgcc gcacctgaac cgtcctgggc tcgtcgaggg cgccgtcgat
     1201 ccgctgctcg acgtgcacgg tggcctcagc ggcatccgct tcggccgtcg agaatgcggt
     1261 gctgagcacc cgcaccggcc cgtgcacctc cagcgcgacg ggccgcacga gccccgcccc
     1321 gttgtaccag cgcgagtcgg agatctcggt gcggtccacg cgcacgctga tcacgaggtc
     1381 gtcgtccggg gcgtacgaga ggagctcggt cagctcgaac gagaacggga catgaccgtt
     1441 cggccggccg ccgaggtggt agccgttgac ccagacgtcg gcgttcttgt acacgccgcc
     1501 gaaggcgagc tcgacgcgcg agtcagaggc cagggcgagc gatgacagcg ggacgtgcgc
     1561 gcggtaccag ccgatgccgc ccggcaggta gccggtgccg ctggagtgct cgggcgcgaa
     1621 gtcctgctcg atgctccagt cgtgcggcac ggcgaccgcg cgccaggacg cgtcgtcgaa
     1681 gcccttcatc caggcctcgc gatgatcggc gagcgcgaag cgccagttgt cgaccgtgaa
     1741 cgtctgcacg ccctcagccc tcctcggatg agtggatgta cctgtcgaag atccgcgcga
     1801 agagcgtgcc gtcgaccaag gcgatgccgc cgaagccgat cagcagccag aggaggccgt
     1861 agcctgtggc ctgaggcgag agcaccgtga ccgtcgcgca gaggacgatc acggtcagag
     1921 cgaagagggt cgtcaggggg tgccgccagg agagcaggcg tgcgttgcgc agggtgtcgc
     1981 ggatgctgcc ctcgaacttg gcgagatacg ggaacaccca cagcgtcatg accacgacga
     2041 ggaagacgat gatgtaccag ccggcgtaca ggacgaactg cacgacggga tcggtgacga
     2101 gagccgagat gaccccatac caggcgacca gcaccgcagc gagaccgagc aggatgcccc
     2161 agatcgcggt cgcctgacgg aggttgccgc ggaacgaccg caggtagtcg ccggtgatgg
     2221 tccggtcggt ccctgtcgcg atgcgcatcg cggtgaagtt cagggcggtc agcgcggccc
     2281 cgatcgtgat caccgggagt gcggtcacga tgaacaggat gttgaggatc atgacgtcgg
     2341 cgatgcgcgt cagcgtcctc atcagcggcg agtcggccgc gaagatgctt cccattcctg
     2401 gtcctctcgt ggtggccggg tgggcccgcg cggcgggccc acccggcagt ggcgtgcagc
     2461 ggctacttga gcgggtggtc cttcgcccag gcgtcgacat ccttctgcag cgacgcgcgc
     2521 acgtcctcgt agccggcgtc cttcagcttc tggcgcatct ccttgatggc cttggccggg
     2581 tcgtcgaact tgccgtagtt cagctgcggg atgtactccg agagcacggc gcccatggcg
     2641 gcgagcgtcg ggtcgatgct gctcttgtcg atgatcagcg tggagtacgg gtagtcgcgg
     2701 gcgaccttgt cgagggcgcc gtagatcttg tcgcggtcgg ggtcgtcggt gaccttcgcc
     2761 ggctcgaact cgtccctgcg gccggcccag aagttggagc ccagcgagtc cttcgacgcg
     2821 tcgtagccct cggggtaccc gagcttgccg tccttcatca cgtagtcggt gccctcgatg
     2881 ccgaagttga gcaggtcgta gtccgccttg tcgttgcgga tcaggtcgta cacctgcagc
     2941 gccttctcgg ggtgcttgga gttcgcgctg acggccatcg cgccgtgcgt cttgatgtcc
     3001 ttgtagaggt tgccgttctc ctggccccag tagaacatct tgggctcgga gccgggctgc
     3061 ttggtggtca tgttgttcca gatctgcccg atgaaggtct gggtgtggtg ctggtcggtg
     3121 ccggacagcc cggagtagaa ctcctcgcgg gtgtcgccgt cgtagttcag cgcgtcctcg
     3181 cgccagacgc cgagcctgtt ccactccttg gccagttcgg cggccttctc cagctcgtcg
     3241 ccctccatgt accaggaggt cacctcgccc gggtcggatt ccgaggtggt gaacggcgcg
     3301 tagttgcccg cgctcacgcc ctgcagcggc atctggtcgg tgtgcccctg caggtagccg
     3361 gtgagcacgg cgtcgttccc gccggtcgag gcgacgtccc acgggtaggc ctcgggcttg
     3421 ttcttcttga tccactggaa gtacttcgtg aagtcctcga aggtgctgat cgtgccgtcg
     3481 gcgaagccgg cctccttcgc ccagtcgccg cggtagaaga acccgtggtt ggtgtactgg
     3541 gtgtagtcgt cctcggggat gaactggatc ttcccgtcga gcttggtgag gtcccagtcg
     3601 ccggacttct cgacctgcgc ccaggtcttc ggcgcgttgg ccttgagcat gtcctcgctg
     3661 agcgggagga aggcgccctt ctcggcgttc tcccatccga acagccagtc ggtcgcggtc
     3721 gtgatcaggt cgaccttgtc gtcacccgag agcagctgca cgttgtactg cgtctgccag
     3781 tcggcccact cgacgtagaa caggtcgagc gtggcgttgg cctgcttctt caggttcgcg
     3841 ttgagcttct tgagcatcgc ctccagacgc ccgttggtcg gcttgtcgcc gagcacgagc
     3901 atcgtgatct tggtcggctt gctcgggtcg tcctcgccgg atgcggtgca gccggccagc
     3961 atggtcattc cgatcccgag ggtcgcgatg accgctccgg ctttgaggat ccttctcttc
     4021 attgcgagtc tcctagtggt gttcagggtg agtgatttca ggggtgaggg gattgcgggg
     4081 tggcgcccgc acatgcgggc agggacgtcg gcgggatcag cccttgaccg ctccgatcgt
     4141 gatgccgctg acgaagtact tctggacgaa cgggtagaag agcaggatcg ggccggtggc
     4201 gaccacggcg ctggccatct tcaaggtgtt ggagggcagg tcgctgatgc tgacgttggc
     4261 tccggcgacg gagttccgca gcgcgttggc cgtattgatg atgttgtaca ggtagtactg
     4321 cagcggcttg aactcgaccg tgctgcccag atagagggac gacaggtacc actcgttcca
     4381 gtaggccagg gcgaggaaga gtccgatggt cgcgagcgcg ggcttcatca tcggcagcgt
     4441 cagctgcgcg aagatccgga agtcgccggc gccgtcgatc ttgcccgact cgacgatctc
     4501 gtagggcacc gtgcgcatga agttcttcat caggatgatc agccagggcg tcatcagcag
     4561 ctgcaggaac accgcgaggt agctgccccg cagatgcagc ccctgcgtga tccacaggaa
     4621 cgtgggggcg agccccgcgg agaacagcgt cgtgaagtag atgaagaacg agatgtggtt
     4681 ccggaacggg aagtcctgcc ggtacagcgc gaaccccgtc atcgcgatga tgaacaggcc
     4741 gatcgccgtg ccgagcacgg tcatggtgac cgtgacgacg taggcgccga tgatctgcct
     4801 gggcgcctgg aagatcagtt cgtaggcggc gaggctgaac cccttcggga gcaggccgaa
     4861 cccgtccttg cggatggcgg cctcgctcga gaacgaggcc gagacgatca gcacgaacgg
     4921 cagcaggcag aacagcgcga agagcgtgat ccacacgtac gagatgccgc gcagccagcg
     4981 gtcgctcgcc gagagcctga tcgagcgcgg ccgcggggag cgggcgaaca ggctcttgtc
     5041 ccgcgtcggc agcagcaggg tcttcgtcgc gccggtgtcg gtgacgggcg ggttcatcgt
     5101 caggtcagaa gagggcatag tccttgttca tccttcgaac gagccagttc gccgtcatga
     5161 cgatggcgaa gccgaacagc gactggtaca gccccacggc cgtggaggtc gtgaagttgt
     5221 tctgcgacat gaccatccgg tacacggagg tctcgatgat gtcggtggtg gagaacagcg
     5281 cagcgttgtt gccgaccagg ttccagaaca gaccgaagtt gccgctcatg atcccgccca
     5341 gcgagaacag gaagaggatg atgaacgtcg gcttcaggct gggcaggatc acgtaccgga
     5401 tgcgctgcca ggcggatgcc ccgtcgatct cggccgcctc gaacagtccc ttgtcgatgc
     5461 ccatgatcgc cgcgaagtag acgatggatc cgtagccggt gctctgccac aggtgcacga
     5521 tgatgatgat cagcggccag agcccggcca tgctgtagat cttgatcggg tcgccgccgg
     5581 tctgcttgat cagcgtgttc agcgctccgc tgtcgtagtt gagcaggttg aaggcgatca
     5641 cgccgatgag cacgaccgag atgaagtagg gcaggaacat gacgccctgc gtgatcttct
     5701 tgaagaactt cagccgcacc tcgttgaaca tgatcgcgat cgcgatctgc agcacgttgc
     5761 cgagcacgat gaacgcgacg ttgtacagga tcgtgttgcg cgtgagatcc cacagcatcc
     5821 cggacttcac gaggaactcg aagttcttga accccacgaa ggggctgccg aagatgccgt
     5881 cgcggtagtt gaagtcggtg aacgcgatcc agatgccggg cagcggcgcg tagttgaaga
     5941 cgaggaagaa cgcgatggcg gggacgcaca tcaggatcag catcctgctg ctcctgatct
     6001 gcccccacag cgacttccgt cgtcgtggtg cttgcgtcgg tatcatcccc cgcctctctg
     6061 cgcgacccgg tcggcgtcga ccgggtggaa tgaacaggga taaaactaac ccctgattaa
     6121 actagggcca cggtagcaga ggccgggtcg gcgagacaag gactttctcg cggcgggtgc
     6181 tgtcagcccg cagacggatg gaccgccggc cagccgtccg tccactccag cggcaggatg
     6241 ccgagggtcg gcacgccctc ggcggcgccg tcgtaccagt ggaaggccag catcccgccc
     6301 gagaccgact ggccgccggg cccgatgcgg tcgccgtcgg tcgtgagcac gaccgtgccg
     6361 ccgtcctcga gcagcctgcc gccgtcggca tccaggtagg ggccgcggac gtccttcgac
     6421 cggccgacga tgatctcgta ggtgctgtcc acgcccttgc agcagaagcc gcgcgaggcg
     6481 aacaggtaga accagccgtg gtggggcacg atgtagggcg cttcgatggc gttctcgacc
     6541 aggccccggt cggccagccg cagcggctcg gcgtcgtcgg cgcgcagtcc gctcggccac
     6601 tcgagctcca ccatccggat gccggaggag aacgacccga acgccatcca gggcgtgccg
     6661 tctgcgtcct cgacgatgcc cgcgtcgatc gcgttgaagt cggtgccggc gctgccgatc
     6721 acctccccgc ggtcgaccca ctcgtagtcg gggtcatcgg ggtcgagggt cgtggtcgtc
     6781 gccagcgcga tcaccgaccg attgctgccg aaggtggacg ccgcgtagta caggtaccag
     6841 gtgccgtcgt gccggtagag ctccggcgcc cacaggttgt cgacgccggg gaccgcctcc
     6901 tccagccacg ccggcttctg ctgccacacc tcccccgcgt actcccagtc cgcgccgtcg
     6961 tcggaacggc ggatctggat gttcccgtcg gcgatgcggc cgtcgccggt ggagtacacg
     7021 aagtcgccgt cgtcggtgtg cacgtaggcg gggtcgtgca cgaacacgtc accgctcagc
     7081 tgcggcggtg cggcgaccgc gggtgcgcag ccggccagcg cggcagat
//
LOCUS       DATMSX010000536         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_168819,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000536 DATMSX010000000
VERSION     DATMSX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168819"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VN041_17045"
     CDS             complement(<1..585)
                     /locus_tag="VN041_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWU30790.1"
                     /translation="MTARHVQVCPVMGTVASAHVIGGAADGDVRAAIESCFDVLRRAD
                     RIFSMYDADSDIRRIARDELRIADADPWVAEVEAACAGAEIETRGLFSAHLGGLFDPT
                     GYVKGWAAERAARGHLLPLLNVPGVQAVGLDVGGDLQVFTAEDSDWTWNIGIVDPSDR
                     AQVIATVPVRNGAVATSGGAERGAHIVGARCWSTY"
     gene            complement(582..1151)
                     /locus_tag="VN041_17050"
     CDS             complement(582..1151)
                     /locus_tag="VN041_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWU30791.1"
                     /translation="MERPMKRIAYAMLATVSGLVLLFSYRTSTGAEIPTANAAAPAAS
                     TGTSGAGTGSGSGTTSNDSASGTDTGSGSGSGSTSGTDGTGSTTGSSTTSSGLKDGTF
                     TGDAADTRYGPVQVQITVSGGSISSVDVVEYPNENPRDRQINQQAVPMLVSETTSAQS
                     AQIDMISGATYTSQGYQQSLQSALDQAKS"
     gene            complement(1191..>2581)
                     /locus_tag="VN041_17055"
     CDS             complement(1191..>2581)
                     /locus_tag="VN041_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HWU30792.1"
                     /translation="ARARRPSAGVRRRRAELWSLGATVVVWTTCLYVVALWVAGGGVQ
                     ALFTSRADLLSSAGRLTGLVASNLLLLQVMLMARVPLFERGFGRDGMTRMHRLVGFWS
                     FALLLTHIVLQTLGYAASAGLNPLVQLWQFVVDYPGMLLAAAGTALVILVALTSMKRA
                     RRRLRYESWHLLHLYGYLGAGLALPHQLWAGSDFTASAAATWYWWGLWILTAVAVIAY
                     RVIVPLVRSRRHLLRVASVARDGADGVTVRMRGEGIARLGAQPGQFFVWRFMDGPGWT
                     RGHPFSLSAAPTGDELTISARLVGDGTSRLAALRPGTRVHVEGPYGGFTGERRRGRRM
                     LMIGAGAGVAPLVALLEGESYAPGEAVIISRDTTPDVALRTDALAGLVRDRGVRHIAL
                     PGRRARSGAVWLPESHSRWNGVDALHHLAPDVIEHEVYVCGPPDWADAVLHGLRAAGF
                     PPERIHREAFAI"
BASE COUNT          392 a          927 c          916 g          346 t
ORIGIN      
        1 gtaggttgac cagcaccttg ccccgacgat gtgcgctccg cgctccgcgc cgcccgaggt
       61 cgcgacggcg ccattgcgca cgggaacggt cgcgatcacc tgagcgcgat cggaggggtc
      121 gacgatgccg atgttccagg tccagtccga gtcctcggcg gtgaagacct gcaggtcgcc
      181 gccgacgtcg agccccacgg cctgcacgcc tggcacgttc agcagcggca ggaggtgccc
      241 gcgggcggcc cgctcggccg cccagccctt cacgtatccg gtcggatcga acagcccgcc
      301 cagatgcgcg ctgaacagcc cgcgggtctc gatctccgct ccggcgcagg cggcctcgac
      361 ctcggccacc caggggtcgg catctgcgat ccgcagctcg tcacgtgcga tgcggcggat
      421 gtcggagtcg gcgtcgtaca tggagaagat gcgatcggca cgacgcagca cgtcgaagca
      481 ggactcgatc gcggccctga cgtcgccgtc ggcggctccg ccgatcacat gcgcactggc
      541 caccgtgccc atcaccgggc agacctgcac gtgccgggcg gtcatgactt cgcctggtcg
      601 agcgcgctct gcagcgattg ctgatacccc tggctcgtgt acgtcgcgcc ggagatcatg
      661 tcgatctgcg ccgactgcgc ggacgtggtc tcgctgacca gcatcggcac ggcctgctgg
      721 ttgatctgcc gatcgcgagg attctcgttc gggtactcga ccacgtcgac ggaggagatg
      781 ctcccgccgc tcaccgtgat ctgcacctgc accgggccgt agcgggtgtc ggccgcgtcg
      841 ccggtgaagg tgccgtcctt caggccggat gaggtcgtgc tggagccggt cgtgctgccg
      901 gttccgtcgg ttccgctcgt ggagccggag ccggagccgg agccggtgtc ggtgccggac
      961 gcggagtcgt tggacgtcgt tcccgagccg gaccctgtcc ccgcaccgga cgtgccggtc
     1021 gacgcggccg gtgcggcggc gttcgccgtg gggatctcgg cccccgtgga ggtgcggtag
     1081 ctgaacagca gcaccagccc gctgacggtg gcgagcatcg cgtacgcgat gcgcttcatc
     1141 ggtcgttcca tgatcttctc ctcgtcgtca ggttccgggt gtcgggatgc tcagatcgcg
     1201 aatgcctcgc ggtggatgcg ctcgggcggg aagccggccg cgcgcaggcc gtgcagcacg
     1261 gcatccgccc agtccggagg gccgcacacg tacacctcgt gctcgatcac gtcgggagcg
     1321 aggtgatgca gagcgtcgac accgttccag cgggagtgcg actccggcag ccacacggca
     1381 ccggaccgcg cgcggcggcc gggcagggcg atgtgacgca cgccgcggtc gcgcaccagc
     1441 ccggccagcg catccgtgcg cagcgccacg tcgggagtgg tgtcgcggct gatgatcacg
     1501 gcctcgccgg gggcgtacga ctctccttcg agcagcgcca ccaggggcgc gacgccggcg
     1561 cccgcgccga tcatcagcat ccgccgccca cgcctgcgct ccccggtgaa tccgccgtaa
     1621 gggccctcga cgtgcacccg agtccctggc cgcagagcgg cgagccgcga cgtcccgtcg
     1681 ccgacgagcc gggccgagat ggtcagttcg tcgccggtcg gcgcagccga gagcgagaag
     1741 ggatgaccgc gcgtccagcc cggcccgtcc atgaagcgcc agacgaagaa ctggcccggc
     1801 tgcgcgccga gccgcgcgat gccctcgccg cgcatccgca cggtcacccc gtcggcaccg
     1861 tcgcgggcga cgctcgcgac gcgcagcaga tgccggcggg agcgcaccag cggcacgatc
     1921 acgcgatagg cgatcaccgc gacggcggtg agtatccaca gcccccacca gtaccacgtg
     1981 gccgcggcgg atgccgtgaa gtccgacccc gcccacagct gatggggcag cgcgagtcct
     2041 gcgccgaggt agccgtacag gtgcagcaga tgccacgact cgtagcgcag ccggcgccgt
     2101 gcgcgtttca tcgaggtgag tgcgacgagg atgacgagcg cggtacccgc ggccgccagc
     2161 agcatccccg ggtagtccac gacgaactgc cacagctgca cgagcgggtt cagcccggcg
     2221 gatgccgcat agccgagcgt ctgcagcacg atgtgcgtga gcagcagcgc gaacgaccag
     2281 aagccgacga gccggtgcat ccgcgtcatc ccgtcgcggc cgaatccgcg ctcgaacagc
     2341 gggacgcgcg ccatcagcat cacctgcagg agcagcaggt tcgaggcgac cagacccgtg
     2401 aggcggccgg ccgagctcag cagatcggcg cggctcgtga acagcgcctg cacacctccg
     2461 ccggcgaccc acagagccac gacgtacagg caggtcgtcc agacgacgac ggtggcgccg
     2521 agactccaca gctcggcacg gcgccgccgg accccggcgc tggggcggcg ggcacgtgcg
     2581 c
//
LOCUS       DATMSX010000537         4633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_46649,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000537 DATMSX010000000
VERSION     DATMSX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46649"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VN041_17060"
     CDS             <1..897
                     /locus_tag="VN041_17060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWU30793.1"
                     /translation="TPPVRAYAKRRGVDLVLVAAEVGDRVITRSDVDAYADRVGAATA
                     GPAGHDDAGASALTVPSAPAGERAQTRIPIKGVRKATAQAMVRSAFTAPHVTCFHTVD
                     VTATMELIEQLRTDRSLSEHRIGPLVLIAKAVCLALGRNPSLNATWDEEAGEIVQNHF
                     VDLGIAAATERGLIVPMIRDAERLSLTGMADALTQLTQTARSGKTSPAELAGGTFSIT
                     NIGVFGIDAGTPILPPGQSGILAVGAVRRQPWEYRGEIALRQMMTLSVSFDHRLVDGA
                     EGSRFLKDVADLLEQPGRAMLF"
     gene            complement(983..1576)
                     /locus_tag="VN041_17065"
     CDS             complement(983..1576)
                     /locus_tag="VN041_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30794.1"
                     /translation="MTTPGTARDRAKAERTDAILSAAAELFAARGYTGVSLEDIGAAV
                     GVSGPAVYRHFAGKQALLGALLIGVSERLVAGGQDVASAAASEQDRMRALVVFHVDFA
                     LGNADVIRVQDRDFVYLTDESRADVRRLQRAYIELWIDSLAALVAEDRDALRLRVQAC
                     FGLLNSTPHSTSTALRARVDTARLLTAMAVSALLADS"
     gene            1674..3227
                     /locus_tag="VN041_17070"
     CDS             1674..3227
                     /locus_tag="VN041_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWU30795.1"
                     /translation="MALTQQELAEELRERLATAALGGPERSRERHVARGKMLPRDRVA
                     RLLDEGSPFIEIAPLAADGLYGGEAPGAGVIAGIGLVHGRHVMVVCNDPTVKGGTYFP
                     MTVKKHLRAQEIALENRLPCLYLVDSGGAFLPKQDEVFPDRDHFGRIFFNQARMSAER
                     IPQLAAVLGSCTAGGAYVPAMSDETVIVRNQGTIFLGGPPLVKAAIGEVVTAEELGGG
                     ELHAKRSGVVDHLAEDDEHALEILRDIVATLPKPAAPAWDVVASRPAAQTGSLHDVVP
                     VDVNAAYDVHEVIDRLVDADSFSEFKPEYGTTLVTGFAVLHGHPIGIVANNGVLFSES
                     ALKGAHFIELCDQRGIPLLFLQNITGFMVGRDAEAGGIAKDGAKMVTAVATTRVPKLT
                     VIIGGSFGAGNYSMCGRAYSPRFLWSWPASRISVMGGPQAASVLATVKEEQLEGRGEP
                     WTPEERAEFEAPIRQQYESQGEPYYATARLWDDGIVDPDRTRDLLGLALDVVSRSPLP
                     EPRFGLFRM"
     gene            3227..>4633
                     /locus_tag="VN041_17075"
     CDS             3227..>4633
                     /locus_tag="VN041_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWU30796.1"
                     /translation="MQMSTFESVLVANRGEIARRVIRTLRDLGIRSVAVYSDADADAP
                     HVREADDAVWIGGAAATDSYLRVDAIIDAARRTGAQAIHPGYGFLSESVALAQACDDA
                     GIVFIGPHVRALEIMGDKARARDHVSQHGVPVVPGFNAAGMDDAQIRARADEVGYPLL
                     VKPSAGGGGKGMEIVRSGEELASALATARRVARAAFGDDAMVLERLVQRPRHIEVQVF
                     GDTHGTVIALGERECTLQRRHQKVIEEAPAAHLPDAVRQRLLDAAVAAAQCVDYVGAG
                     TVEFLVEADSVDDIFFIEMNTRLQVEHPVTEEVTRLDLVALQVQVAAGGALPEPPEVR
                     GHAVEARVYAESPERGFLPSTGHVLLFDAPRGVRVDAAVETGSEVTGFYDPMIAKVIA
                     FGPDRESALRRLDAALARTVVLGVETNIAFLRALSTHERVVAGDLDTGLIETMLPLPA
                     QQPTASMLAAAAHAVHRPR"
BASE COUNT          699 a         1631 c         1627 g          676 t
ORIGIN      
        1 acgcccccgg tgcgtgccta cgcgaagcgt cgcggagtgg acctcgtgct ggtcgccgcc
       61 gaggtggggg atcgcgtgat cacccgctcc gacgtcgacg cgtatgcgga ccgtgtcgga
      121 gctgcgacgg cgggccctgc cgggcacgac gacgccgggg cgtccgcact gaccgttccg
      181 tccgcaccgg cgggcgagcg tgcacagacc cgcatcccca tcaagggcgt gcggaaggcg
      241 accgcccagg ccatggtgcg cagcgccttc accgccccgc acgtcacttg cttccacacg
      301 gtcgacgtga cggcgacgat ggagctgatc gagcagctgc gcacggaccg gtcactgtcc
      361 gagcaccgca tcggcccgct cgtgctgatc gcgaaggccg tctgcctcgc actggggcgc
      421 aatccctctc tcaacgcgac ctgggacgag gaggcgggcg agatcgtgca gaaccacttc
      481 gtggatctgg gtatcgccgc agccaccgag cgcggcctca tcgtcccgat gatccgcgac
      541 gccgagcgcc tgtcgttgac ggggatggcc gatgccctga cccagctcac gcagaccgcg
      601 cgctccggta agacgagccc ggccgagctc gcgggcggga cgttctcgat cacgaacatc
      661 ggcgtcttcg gcatcgacgc cggtacgccg atcctgccgc ccgggcagtc cggcatcctc
      721 gctgtgggtg cggtcaggcg tcagccgtgg gagtacaggg gagagatcgc gctgcgtcag
      781 atgatgacgc tgagtgtgtc gttcgatcac cgtctggtgg acggcgccga gggatcgcgc
      841 ttcctgaagg acgtcgcaga cctgctggag cagcccggcc gggcgatgct cttctgagcg
      901 actgacagga cgagcggatg ctgagggcgg gactcccggg agtcccgtcc tcagccgccc
      961 gtgccctcag ccgcccgcga cctcagctgt ccgcgaggag ggcggagacg gccatcgccg
     1021 tgagcaggcg tgcggtgtcg acgcgggctc gcagcgccgt gctggtggag tgcggcgtgg
     1081 agttcagcag cccgaagcag gcctggaccc gcaatcgcag cgcgtcgcgg tcctcggcga
     1141 ccagcgccgc cagcgaatcg atccagagct cgatgtaggc gcgctgcagt cgtcgcacgt
     1201 ccgcgcggct ctcgtctgtg agatagacga agtcgcggtc ctgcacgcgg atgacgtcgg
     1261 cattgcccag ggcgaagtcg acgtggaaca ccaccagtgc ccgcattcgg tcctgctccg
     1321 aggccgccgc ggaggccacg tcctggccgc cggccaccag ccgctcgctc actccgatga
     1381 gaagcgcgcc gaggagtgcc tgcttgcctg cgaagtggcg gtagaccgca ggtcccgaga
     1441 cgccgaccgc ggcgccgatg tcctccaggc tcacgccggt gtagccgcgc gcggcgaaga
     1501 gctccgcggc cgcggagagg atggcgtcgg tgcgctcggc cttcgcacga tcgcgggcgg
     1561 tcccaggcgt tgtcatttcg gttaatcctc gctaacctga attatccggt taatgaacac
     1621 taaccaactc agcgtgaaat atcagcactg aggtgtgtcg aggaggaccc acgatggctc
     1681 tcacccagca ggaactggcc gaagagctgc gcgagcgact cgccacggcc gctctgggcg
     1741 gcccggagcg ctcccgggag aggcacgtcg cccgcggcaa gatgctcccg cgggaccgcg
     1801 tcgcgcggct gctggacgag gggagcccct tcatcgagat cgcgcccctc gccgcggacg
     1861 gcctctacgg cggcgaggcg cctggtgcgg gcgtgatcgc ggggatcggg ctcgtgcacg
     1921 gcaggcacgt gatggtggtc tgcaacgacc cgaccgtcaa gggtggcacg tacttcccga
     1981 tgaccgtcaa gaagcacctg cgcgcgcagg agatcgcgct ggagaaccga ctgccgtgtc
     2041 tgtacctggt cgattcgggc ggcgccttcc tgcccaagca ggacgaggtc tttcccgatc
     2101 gtgaccactt cggccgcatc ttcttcaacc aggcccgcat gtctgcggag cgcattccgc
     2161 agctcgccgc cgtcctgggg tcctgcacgg cgggcggcgc atacgtcccg gcgatgagcg
     2221 atgagaccgt gatcgtgcgc aaccagggca cgatcttcct cggcgggccg cccctggtga
     2281 aggccgcgat cggcgaagtg gtgacggcgg aggagctcgg cgggggagag ctgcatgcca
     2341 agcgctcggg cgtggtcgac cacctggccg aggatgacga gcatgccctg gagatcctgc
     2401 gcgacatcgt cgcgacgctg ccgaagcccg cggcgcccgc ctgggatgtc gtcgcctccc
     2461 gcccggctgc gcagacgggg agcctgcacg acgtcgttcc cgtcgatgtg aacgccgcct
     2521 acgacgtgca cgaggtgatc gaccggctgg tcgacgcaga cagcttctcg gagttcaagc
     2581 cggagtacgg cacgacgctc gtcaccgggt tcgccgtgct gcacgggcat ccgatcggga
     2641 tcgtcgccaa caacggcgtg ctcttcagcg agtccgcact gaagggcgcg cacttcatcg
     2701 agctgtgcga ccagcggggc atcccgctgc tgttcctgca gaacatcacc ggcttcatgg
     2761 tcggccgcga cgcggaggcc ggcggcatcg ccaaggacgg cgcgaagatg gtcaccgccg
     2821 tcgccaccac gcgcgtcccc aagctcacgg tcatcatcgg cggctccttc ggagccggca
     2881 actactcgat gtgcgggcgg gcctactcgc cccggttcct gtggagctgg ccggccagcc
     2941 gcatctcggt gatgggcggt ccgcaggcgg cgtccgtgct cgccacggtc aaggaggagc
     3001 agctcgaagg acgcggtgag ccctggacac ccgaggagcg cgcggagttc gaggccccga
     3061 tccgccagca gtacgagagc cagggcgagc cctactacgc caccgcccgt ctctgggacg
     3121 acggcatcgt cgaccccgac cggacccgcg acctgctggg cctcgccctc gacgtcgtct
     3181 cccgcagccc cctgcccgaa ccgcgcttcg gcctcttccg gatgtgatgc agatgagcac
     3241 cttcgaatcc gtcctcgtcg ccaaccgcgg cgagatcgcc cgccgcgtca tccgcaccct
     3301 gcgcgacctc ggcatccgca gcgtcgccgt gtacagcgac gcggatgccg acgccccgca
     3361 cgtccgcgag gccgatgacg ccgtgtggat cggcggagca gccgcaacgg actcctacct
     3421 gcgcgtggac gccatcatcg acgccgcccg gcgcaccgga gcgcaggcga tccaccccgg
     3481 ctacggcttc ctgtccgaga gcgtcgcgct cgcgcaggcc tgcgacgacg ccggcatcgt
     3541 gttcatcggc ccgcacgtgc gagccctgga gatcatgggc gacaaggccc gtgcccgcga
     3601 ccacgtgtcg cagcacggcg tgcccgtcgt gcccggattc aacgcggcgg gcatggacga
     3661 cgcgcagatc cgcgcccgtg ccgatgaggt cggctacccg ctgctcgtca agcccagcgc
     3721 cggcggcggc ggaaagggca tggagatcgt gcgctcgggc gaggagctgg catccgctct
     3781 cgcgaccgcc cgacgggtcg ccagggccgc cttcggcgac gacgccatgg tgctggagcg
     3841 cctggtgcag cgcccgcggc acatcgaggt gcaggtcttc ggcgacaccc acggcacggt
     3901 catcgcgctg ggcgagcgcg agtgcacgct gcagcgccgt caccagaagg tgatcgagga
     3961 ggcgccggcc gctcacctgc cggatgccgt gcggcagcga ctgctcgatg ccgcggtcgc
     4021 cgcggcgcag tgcgtcgact acgtcggcgc cgggactgtg gagttcctcg tcgaggccga
     4081 ttcggtcgac gacatcttct tcatcgagat gaacacccgc ctccaggtgg agcacccggt
     4141 gaccgaggag gtcacccggc tggacctggt cgcactgcag gtgcaggtcg ccgcgggtgg
     4201 tgcgctgccc gagcctcccg aggtgcgcgg gcatgccgtg gaggcacgcg tctacgccga
     4261 gtcccccgag cggggcttcc tgccctcgac ggggcacgtc ctgctgttcg acgcgccgcg
     4321 tggcgtgcga gtggatgctg cggtggagac gggatccgag gtcaccggct tctacgaccc
     4381 gatgatcgcg aaggtgatcg cgttcggacc cgaccgggag agcgccctgc ggcggctgga
     4441 tgctgccctc gcacggacgg tcgtcctggg ggtggagacc aacatcgcct tcctgcgtgc
     4501 tctcagcacc cacgagcgag tcgtcgccgg cgacctcgac accgggctga tcgagaccat
     4561 gctgccgctg ccggcgcagc agccgaccgc atcgatgctc gcagccgccg cacacgccgt
     4621 gcaccgcccg cgt
//
LOCUS       DATMSX010000538         8762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_36536,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000538 DATMSX010000000
VERSION     DATMSX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36536"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VN041_17080"
     CDS             <1..540
                     /locus_tag="VN041_17080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWU30797.1"
                     /translation="GMSAVLGGDADEVLALLDDLELAPANYNVAGQIVAAGALESLAK
                     LAESAPRGVRVMPLQVAGAFHTSYMGPAVETLRAAVDDVEANDPTITLWSNRDGAVVA
                     DGRDALDRIVTQVSSPVRWDLCMESMAQAGITGFIEVAPAGALVGLAKRGLKGVPNVA
                     VKTPDDLDAAVALLNGEAA"
     gene            537..1538
                     /locus_tag="VN041_17085"
     CDS             537..1538
                     /locus_tag="VN041_17085"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWU30798.1"
                     /translation="MSATITQATGAAHTRIYSVGAARGENAVPNDDLVGPIDSSDEWI
                     RQRTGIITRARAVPETDAIDLSSAAAAEAVQRSGVDPADIDLVIVATISNPKQTPSVS
                     AIVADRIGANPAAAYDVNAACAGYAYAVAQADALIRAGAAHYAVVVGAEKLSDIVDPT
                     DRSISFLLGDGAGAVVIGPSETPGISATVWGSDGSKADLVGMNHTLTEFRDGTSPWPT
                     LRQEGPRVFRWAVWEMAKVAREALEKAGIEASDLAAFIPHQANLRIVEEFAKQLKLPE
                     TTVIARDIETTGNTSAASIPLATHRLLEEHPELSGGLALQIGFGAGLVFGAQVVVLP"
     gene            1602..1850
                     /locus_tag="VN041_17090"
     CDS             1602..1850
                     /locus_tag="VN041_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWU30799.1"
                     /translation="MALTTDEVLAGLAELVTDETGIDASEVAMEKSFTDDLDIDSISM
                     MTIVVNAEEKFGVTIPDDEVKNLKTVADAVNFIVAGQE"
     gene            1973..3208
                     /locus_tag="VN041_17095"
     CDS             1973..3208
                     /locus_tag="VN041_17095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWU30800.1"
                     /translation="MTKRIVVTGIGATSALGGTAPENWANLLAGKSGTRTLEHDWIEK
                     YQIPVHFAAEATVRPEEVLPRHEAKRLDPSTQFAMIAAREAWEDAGSPEVAPERLGID
                     WATGIGGVWTLLDAWDTLREKGPRRVMPLTVPMLMPNAGAGNLSLHFNAKAFARTVVS
                     ACASSTESIANAYDHLQDGLADVIIAGGAESAIHPITMASFASAQALSRRNDDPATAS
                     RPGAIDRDGFVMGEGGAALVLETEEHAKARGAKIYAYLVGGGVSADSYHITGNDPEGA
                     GAARAVEQALQQAGASADEVSHINAHATSTPVGDPNEYEALKRVFGDRIEEIPVSATK
                     ASTGHLLGGTGALEAVYTILALRDRVAPPTINMTEPDPAVPFKLSTSPQPLGDGDLLA
                     ISNSFGFGGHNAVVAFRSA"
     gene            complement(3272..3934)
                     /locus_tag="VN041_17100"
     CDS             complement(3272..3934)
                     /locus_tag="VN041_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30801.1"
                     /translation="MNARSILMPIAAASRRELVERLTQLLSGLILYGAAMALMVRAGT
                     GVAPWDVLALGVADRTGLAFGVVTCLVAAVVLLLWIPLRQRLGIGTLLNAVLIGPSAD
                     LTLGLVPPAPSVWIGVPMFFGGLVLLAFATGLYISADFGPGPRDGLMTGLVARTGRPV
                     WMVRTVLEGGVLLIGFLLGGAVGVGTVLFAFGVGPLVGWFLAMFTRIRGSRALALARP
                     VA"
     gene            4030..5433
                     /locus_tag="VN041_17105"
     CDS             4030..5433
                     /locus_tag="VN041_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWU30802.1"
                     /translation="MTSRLVDQLGAQSVAGATAASLADQIRALILDGRITVGERLPSE
                     RALATELRRSRSTVTRAYDLLTAAGYATRVHGGGTRVALPNGSVAPTSSDDAGAIDLS
                     IASMDSTPGLYDATVRSLPRLAALRGTSGYSLQGLPELRDAVAQRFTQRGVPTSAEEI
                     MITSGALNAMNLILAAIGRRGERALIEQPTFPHALESLRRHGYRLVPTPVDVDGWDTA
                     HLMDLLLRSRPHMAYLIPDFHNPTGATLPDGARPRVAATARSAGTHLIVDETTAELDI
                     DRSWTPVPMAAFGPHVLTIGLMSKIAWGGMRLGWIRAEPGIIARLLAVRPSFELGTAL
                     LEQCIAIELLDDMPALLGHVRERLHAGREAVAAGVARLHGLTMPTTPGGLSTWLDLGA
                     PISTRLALRAREHGLILPPGPRFALGGVLERRLRIPITLTPERTAAAMDRLAAAWTAV
                     RDHAVESALPLQRTAVI"
     gene            complement(5575..6078)
                     /locus_tag="VN041_17110"
     CDS             complement(5575..6078)
                     /locus_tag="VN041_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HWU30803.1"
                     /translation="MATGYARGVVYVHSAPRALCPHLEWAVGRAIGRAVSFDWSDQPV
                     QSGTRRAEFYWDGPVGTGSAIATAIRGWEHLRFEVSEDPTAGYDGGRWMHTPDLGIHY
                     AQTDSAGNVVIGEDRIRYAMEIAAGSMVELQRELDVALGAAWDEELEPFRHAGDDARV
                     VWLHKVG"
     gene            complement(6256..7029)
                     /locus_tag="VN041_17115"
     CDS             complement(6256..7029)
                     /locus_tag="VN041_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30804.1"
                     /translation="MGYIKSEALEEKGFVILDSYAQELDPKEWLDLEYNDWKSSGDTR
                     FAPLASAKGEIECNGFWNHKPPRTDKDGVWIDSQVEKAPNLTRRAKEPGANVGRCRVI
                     ELQPTPYGDCLYNLHQDDNNRLNPDGTGWVVRGFFNLTDDKDSFFVLRENRTDPQGEV
                     RIALPAGAQLIIDTQRLWHATTHNGTEPRYNLITSWTSGPELDAYIEKYHGTDQHTQF
                     EVPQDVLEHGYAEQARKDAARAAYYAAKGQQAKLAMSEA"
     gene            7202..>8762
                     /locus_tag="VN041_17120"
     CDS             7202..>8762
                     /locus_tag="VN041_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HWU30805.1"
                     /translation="MATATNVDATATGTIEWLAAPTTNIVVPVYQRQYRWDIGACEKL
                     LSDVRIVAGEGTDHRHFIGSILSAEDTAGGDLVLIDGQQRVTTIMLLIAALHHTIRET
                     DPVMASDLERVLVRADDASRTKLRPHHAWADLYESVVLDRRDDGDRESRFDDNYAFFR
                     SQVHADEAAGIWQGLQRLEHVSITLGSSANAQQIFESLNSTGQPLRDHELIHNYILMG
                     LSHAEQLDVEEQFWLPIERHTGETIGAFWRHYLMMTMGREIDAAGEHGVYAAFRGSFP
                     RLDVEHLRADAGLWREYAEIYGILLDPSGEPDDGIRLQLEHVNAFGRTSYPLVMSAYG
                     DHRRGLISREELIEVLEWVQALFLRRAIVGLPTDRLIARLCRARAQGRESLMRAIARI
                     TPSDERVSAVLKYGELPHPAYVLGRIEGVQDVTDFDVEAIVPPVPATTWSGDGIRPWT
                     EYSEDEQNSHRALSPTLGNLTLLEESLTMRVFGASFPDKRDQAYARSAVPATRELADV
                     AAWGTAAISART"
BASE COUNT         1400 a         3197 c         2868 g         1297 t
ORIGIN      
        1 ggcatgagcg cggttctcgg cggcgacgcc gacgaggtgc tcgccctcct cgacgacctg
       61 gagctcgccc ccgccaacta caacgtcgcc gggcagatcg tggccgcagg cgccctggag
      121 agcctcgcga agctcgcgga gagcgctcca cgcggcgtcc gcgtcatgcc gctgcaggtg
      181 gccggcgcct tccacacctc ctacatggga cccgcggtgg agacgcttcg cgccgccgtc
      241 gacgacgtcg aggcgaacga cccgacgatc actctctggt cgaaccgcga cggcgccgtc
      301 gtggccgacg gacgcgacgc tctcgaccgc atcgtcaccc aggtgtcctc gccggtccgc
      361 tgggacctgt gcatggaatc gatggcccag gcgggtatca ccggcttcat cgaggtcgcc
      421 ccggccggcg cgctcgtcgg cctcgcaaaa cgcggcctga agggcgtgcc caacgtcgcc
      481 gtgaagaccc ctgacgacct cgacgcagca gtcgcgctgc tgaacggaga agcagcatga
      541 gcgccaccat cacacaggcc accggagccg cgcacacccg gatctactcc gtcggtgccg
      601 cgcgcgggga gaacgccgtc ccgaacgacg acctggtcgg ccccatcgac tccagcgatg
      661 agtggatccg ccagcgcacc ggcatcatca cgcgcgctcg cgccgtgccc gagacggacg
      721 cgatcgatct ctcctccgcc gcggcggccg aggccgtgca gcgctccgga gtcgacccgg
      781 ccgacatcga cctcgtcatc gtcgccacca tcagcaaccc caagcagacg ccctcggtgt
      841 cggcgatcgt cgccgaccgc atcggcgcga atcccgctgc cgcgtacgac gtcaatgccg
      901 cctgtgccgg ctacgcctat gccgtggcgc aggccgacgc gctcatccgc gccggcgccg
      961 cgcactacgc cgtcgtggtc ggcgcggaga agctgtccga catcgtcgac ccgaccgacc
     1021 gcagcatctc cttcctgctc ggagacggcg cgggcgcggt ggtcatcggc cccagcgaga
     1081 cccccggcat ctccgccacc gtgtggggct ccgacgggtc caaggccgac ctcgtcggca
     1141 tgaaccacac gctcaccgag ttccgtgacg ggacctcccc gtggccgacc ctccgccagg
     1201 aaggaccccg cgtgttccgc tgggcggtct gggagatggc caaggtcgcc cgtgaggccc
     1261 tcgagaaggc cggcatcgaa gcatccgacc tcgccgcctt catcccgcac caggcgaacc
     1321 tgcgcatcgt ggaggagttt gccaagcagc tgaagctccc cgagaccacc gtcatcgcac
     1381 gtgacatcga gacgaccggc aacacctcgg ccgcctcgat cccccttgcc acccaccgtc
     1441 tgctcgaaga gcaccccgag ctcagcggtg gtctggcgct gcagatcggc ttcggcgcag
     1501 ggctcgtctt cggcgcgcag gtggtggtgc tcccctgatc gggagcgcct tccctagact
     1561 gttcaccggt tccgaaacca ttccgagaaa gaggaaacac catggctctc accaccgacg
     1621 aggtcctcgc aggacttgct gagctggtca ccgacgagac cggcatcgac gcatccgagg
     1681 tcgcgatgga gaagtcgttc accgacgacc tcgacatcga ctcgatctcg atgatgacga
     1741 tcgtcgtcaa cgccgaggag aagttcggcg tgaccatccc cgacgacgag gtcaagaacc
     1801 tcaagacggt cgccgacgcc gtcaacttca tcgtcgccgg ccaggagtaa gcaccaccgc
     1861 ggatgccacc cccgccggcc ctgcactcga caggctcagc agccggtcga tgggcgcggg
     1921 gcgtggcatc ctccttttcc ctgaaccacc tccctcctca cgaaggacct tcatgaccaa
     1981 gcgcatcgtc gtcaccggta tcggcgccac gtccgctctg ggcggcaccg ccccagagaa
     2041 ctgggccaat ctgctcgcag gcaagtccgg tacgcgcacg ctggagcacg actggatcga
     2101 gaagtaccag atccccgtgc acttcgccgc cgaggcgacc gtccgtcccg aggaggtgct
     2161 cccccgtcac gaggccaagc gcctcgaccc ctcgacgcag ttcgcgatga tcgccgcccg
     2221 cgaggcctgg gaggacgccg gttcccccga ggtcgccccc gagcgcctcg gcatcgactg
     2281 ggccacgggc atcggcggcg tgtggacgct gctggacgcc tgggacacgc tgcgcgagaa
     2341 gggaccgcgc cgggtcatgc cgctgacggt tccgatgctg atgcccaacg ccggcgcggg
     2401 aaacctgtcg ctgcacttca acgccaaggc cttcgcacgc acggtcgtca gcgcgtgcgc
     2461 ctccagcacg gagtcgatcg cgaacgccta cgaccacctg caggacggtc tcgccgacgt
     2521 gatcatcgcc ggtggcgcgg agtccgcgat ccacccgatc accatggcgt ccttcgcgtc
     2581 ggcacaggcc ctgtcccgtc gcaacgacga cccggccacc gcctcccgcc ccggcgccat
     2641 cgaccgcgac ggattcgtca tgggcgaggg cggtgcggca ctggtactcg agaccgagga
     2701 gcacgccaag gcccgcggtg cgaagatcta cgcctacctg gtgggcggcg gcgtctccgc
     2761 cgactcgtac cacatcaccg gcaacgaccc cgagggcgcg ggcgcggccc gtgccgtcga
     2821 gcaggcgctg cagcaggcag gtgcctccgc ggacgaggtc tcgcacatca acgcgcacgc
     2881 cacgtccacc ccggtcggcg acccgaacga gtacgaggcg ctcaagcgcg tgttcggcga
     2941 tcgcatcgaa gagatccccg tctccgcgac caaggcgtcg accgggcacc tgctgggcgg
     3001 caccggcgcg ctcgaggccg tgtacacgat cctcgcgctg cgcgaccgtg tcgcgccgcc
     3061 gaccatcaac atgaccgagc ccgacccggc ggtcccgttc aagctctcga cctcgccgca
     3121 gccgctcggc gacggcgatc tgctcgcgat cagcaactcg ttcggcttcg gcggccacaa
     3181 cgccgtcgtc gcgttccgca gcgcctgagc cggctcctgc agcagtacgc ccccggggat
     3241 tccccggggg cgtactgctt ttctcgcgcc gtcaggccac cggacgagcg agcgcaaggg
     3301 cgcgggatcc ccggatgcgg gtgaacatcg cgaggaacca gccgacgagc ggccccactc
     3361 cgaacgcgaa cagcacggtc cccacgccga ccgcgccgcc cagcagaaag ccgatgagca
     3421 ggacgcctcc ctcgagcacg gtgcgcacca tccacaccgg acgtcccgtt cgcgccacca
     3481 gcccggtcat caatccatcg cggggcccgg gaccgaagtc ggcggagatg tacagtccgg
     3541 tggcgaacgc cagcagcacc agcccgccga agaacatggg cacgccgatc cagaccgagg
     3601 gcgcgggagg aaccagcccg agcgtgagat cggcgctggg cccgatcagc acggcgttga
     3661 gcagcgtgcc gatccccagc cgctgacgca gcggtatcca cagcaggagc acgacggcgg
     3721 ccaccaggca ggtcaccacc ccgaacgcga ggccggtgcg atcggcgacg cccaaggcca
     3781 gcacgtccca gggcgccacg ccggtgcccg cccgcaccat cagggccatg gccgcgccgt
     3841 acaggatgag cccggagagc agttgcgtga gtcgctcgac gagttcgcgt cggctggcgg
     3901 ctgcgatggg catgaggatc gaccgtgcgt tcatgagtcc atgctgaggc cgcatccgac
     3961 gccgccgacg agaccactcc tcacaaagtg gcctgctgat ctcggtccac tttgcggcat
     4021 gctggggaca tgacatcacg actggtcgat cagctcggcg cccagagcgt ggctggggcg
     4081 actgccgcga gtctggcgga tcagatccgc gccctgatcc tggacggacg gatcaccgtc
     4141 ggcgagcgac tgcccagtga acgcgcgctg gcgacggagc tgcgccgctc acgctccact
     4201 gtgacccgag cctacgatct gctcacggcc gccggatacg cgacgcgtgt gcacggaggc
     4261 ggcacccgcg tggcgctgcc gaacggatcc gtcgcgccga cctcgtccga cgacgcgggc
     4321 gcgatcgacc tctccatcgc gtcgatggac tccacccccg ggctctacga cgccaccgtg
     4381 cggtcactgc cccggctcgc cgcgctgcgc ggcacgagcg gctactcgct gcaggggctg
     4441 ccggaactgc gggacgccgt cgcgcagcgc ttcactcagc gcggcgttcc gacatccgcc
     4501 gaggagatca tgatcacctc gggggcgctg aacgccatga acctcatcct ggcggcgatc
     4561 ggtcgacgag gagagcgcgc cctgatcgag cagcccacct tcccgcacgc gctggagtcg
     4621 ctgcgccgcc acggctatcg gctggtgccg accccggtcg atgtcgatgg ctgggacacc
     4681 gctcacctca tggatctgct gctgcgcagt cgtcctcaca tggcgtacct catccccgac
     4741 ttccacaacc ccaccggggc caccctgccc gatggtgcac gcccgcgggt cgccgccaca
     4801 gcacgcagcg cgggaacgca cctcatcgtc gacgagacga cggcggaact cgacatcgac
     4861 cggagctgga ccccggtgcc gatggcagca ttcggtcccc acgtgctcac gatcggattg
     4921 atgtcgaaga tcgcctgggg cggtatgcgc ctgggctgga tcagagcgga acccgggatc
     4981 atcgcgcgac tgctcgccgt tcgcccctcg ttcgaactgg ggaccgcgct gctggagcag
     5041 tgcatcgcca tcgaactgct cgacgacatg cctgcgctcc tcgggcatgt gcgcgagcgg
     5101 ctgcacgcgg gccgcgaggc cgtggccgcg ggcgtcgccc gactgcacgg tctcacgatg
     5161 cccaccaccc ccgggggcct ctcgacctgg ctggatctcg gcgcaccgat ctcgacgcgg
     5221 ctggcgctcc gcgcgcggga gcacgggctg atcctgccgc ccggtccgcg cttcgcgctg
     5281 ggaggggtcc tggagcgacg cctccggatc ccgatcacgc tcacgcccga gcgcacggcc
     5341 gcggccatgg accggctggc cgcagcatgg accgccgtac gcgaccacgc cgtcgagagt
     5401 gccctccccc tccagcgcac agcggtgatc tgaggcggat cgtccgggca tgacgaagcc
     5461 ccctgcttcc ccgcaggggg ctccagtcgt tatgcggagg attcgctttc tcggcatccg
     5521 ggaggtgatc accggatccc gttactcggg cctcctccgc acgtctctgg gtcgctaccc
     5581 gaccttgtgg agccacacga cacgcgcatc gtcgccggca tgacggaagg gctccagctc
     5641 ctcgtcccac gcggcgccga gggcgacgtc gagctcccgc tgcagctcga ccatgctgcc
     5701 cgccgcgatc tccatcgcgt agcggatgcg gtcctcgccg atcaccacgt tgcccgcgga
     5761 gtccgtctgc gcgtagtgga tgccgaggtc gggcgtgtgc atccagcgac cgccgtcgta
     5821 cccggccgtc gggtcctcgg agacctcgaa gcgcaggtgc tcccagccgc ggatggccgt
     5881 cgcaatggcg gagcccgtac cgaccggccc gtcccagtag aactcggctc gtcgggtgcc
     5941 cgactgcacc ggctgatcgc tccagtcgaa gctgacggcg cgaccgatag cgcgtccgac
     6001 cgcccattcg aggtgcgggc agagcgcgcg tggggcggag tggacgtaca ccactccgcg
     6061 tgcgtatccc gtcgccatga tctctccgtt tcatcaggtg cgtcttcccc tacgacctga
     6121 ccgaagaacc tcggcgagct atgcggttat gaggcgatta tcgcccagat cccggggaat
     6181 gacaagcatg tgattccccg gatgcaacag cggccccgat ccgcgagggg accggggccg
     6241 ctgcgatgcg gagactcagg cctcgctcat ggcgagcttg gcctgctggc ccttggccgc
     6301 gtagtaggcg gcacgggcgg cgtccttgcg agcctgctcg gcgtagccgt gctcgagcac
     6361 gtcctgcgga acctcgaact gcgtgtgctg gtcggtgccg tggtacttct cgatgtaggc
     6421 gtccagctcc ggacccgagg tccacgaggt gatcaggttg tagcgcggct cggtgccgtt
     6481 gtgcgtggtc gcgtgccaca ggcgctgcgt gtcgatgatc agctgcgcgc cggcgggcag
     6541 cgcgatgcgc acctcgccct gggggtcggt gcggttctcg cgcaggacga agaagctgtc
     6601 cttgtcgtcg gtcaggttga agaagccgcg cacgacccag ccggtgccgt ccgggttgag
     6661 gcggttgttg tcgtcctggt ggaggttgta gaggcagtcg ccgtaggggg tgggctgcag
     6721 ctcgatcacg cggcagcggc cgacgttcgc acccggctcc ttggcgcggc gcgtcaggtt
     6781 gggagccttc tcgacctgcg agtcgatcca gacgccgtcc ttgtcggtgc gcggcggctt
     6841 gtggttccag aagccgttgc actcgatctc gcccttggcg ctcgcgagcg gagcgaagcg
     6901 ggtgtcaccc gaggacttcc agtcgttgta ctccaggtcc agccactcct tggggtcgag
     6961 ctcctgggcg tagctgtcga ggatgacgaa tcccttttcc tcgagggctt cggacttgat
     7021 gtatcccatg atccgagtca tgtcctttcg tagcgggcca gcacgtttta acaggcccta
     7081 cttaggtaag cctacatcgg cctcctgaac acgccccaga ggccgaccgg agaaaatcgg
     7141 gccggactag actcgtcccg gcagcgttca actgcccgct gatcggagga ttcacgcagt
     7201 gatggcaacg gcgaccaatg tcgacgcgac cgcgacgggc accatcgaat ggctcgcagc
     7261 ccccacgacc aacatcgtcg tcccggtgta tcagcgccag taccgctggg acatcggcgc
     7321 ctgcgagaag ctgctctccg atgtccgcat cgtcgcgggc gaagggaccg accaccggca
     7381 tttcatcggg tcgatcctgt ccgccgagga caccgccggc ggagatctgg tgctgatcga
     7441 cggacagcag cgcgtgacca cgatcatgct gctgatcgcg gctctgcacc acaccatccg
     7501 cgagaccgac ccggtgatgg catcggatct ggagcgggtc ctggtgcgcg ccgatgacgc
     7561 ctcgcgcacc aagctgcgcc cgcatcatgc ctgggccgat ctctacgagt ccgtggtcct
     7621 ggaccgacgc gacgacggcg accgggagtc gcgcttcgac gacaactacg cgttcttccg
     7681 gagccaggtg cacgccgacg aggccgcagg catctggcag ggcctgcagc gactggagca
     7741 cgtctcgatc acgctcggtt cctcggccaa cgcgcagcag atcttcgaga gcctgaactc
     7801 caccggccag ccgctgcggg accacgagct catccacaac tacatcctga tgggcctctc
     7861 gcacgcggag cagctcgacg tcgaggagca gttctggctg ccgatcgagc ggcataccgg
     7921 cgagaccatc ggcgcgttct ggcggcatta cctgatgatg acgatgggcc gcgagatcga
     7981 cgccgccggc gagcacggcg tgtacgccgc gttccgcggg tcgttccctc ggctcgacgt
     8041 cgagcacctg cgcgcggacg ccggtctctg gcgcgagtac gccgagatct acggcatcct
     8101 gctggacccc tccggagaac cggacgacgg catccggctg cagctggagc acgtgaacgc
     8161 cttcgggcgc acctcctatc cgctggtgat gagtgcctac ggcgaccatc gacgcgggtt
     8221 gatcagccgc gaggagctga tcgaggtgct cgagtgggtg caggcgctgt tcctgcgccg
     8281 cgccatcgtc ggcctgccca ccgatcgcct gatcgcccgc ctctgccgcg cccgcgcgca
     8341 gggccgggag tccctcatgc gcgcgatcgc ccggatcacc ccgtctgacg agcgcgtcag
     8401 cgccgtgctc aagtacggcg agcttcccca cccggcatat gtgctcggcc gcatcgaggg
     8461 cgtgcaggac gtcaccgact tcgacgtcga ggcgatcgtg ccgccggtgc ccgccaccac
     8521 gtggagtggc gacggcatcc gcccgtggac cgagtacagc gaagacgagc agaacagcca
     8581 ccgcgcactg tccccgacgc tcggcaacct cactctgctc gaggagtcgc tcaccatgcg
     8641 cgtgttcggg gcatccttcc ccgacaagcg cgaccaggcc tacgcccgca gcgccgtccc
     8701 cgccacgcgc gagctggcgg acgtcgcagc ctggggcacc gccgcgatct cggcgcggac
     8761 cg
//
LOCUS       DATMSX010000539         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_27004,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000539 DATMSX010000000
VERSION     DATMSX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27004"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VN041_17125"
     CDS             <1..1181
                     /locus_tag="VN041_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HWU30806.1"
                     /translation="DTAHGAVVLDSRRVDAARRSALQGGVGHNRARELFLEYVVDDLV
                     DELEQQTAKEMSDFEEELKEVGVDLDRMFARQHGEPEPHGLESSPGGLDIDWDRIRDD
                     LLDDAGIGRIVSEVWPALRAEDVLLGLLADPEALALAAPELSDEQIASVVSGAGAGWS
                     RADLAALDEARALVDGIPEVTYGHVVVDEAQQLSEMEWRMLMRHCPSRSMTIVGDLAQ
                     AGPTTTITRWEEALEPFVDGRYAHHTLTVNYRTTAEILDATRPVLARIAPSQRLSNSI
                     RHGEVPSHHTVADVDRALGDLVARVRDEHPQGLIGIIAAPRRIRALDAGFAGDGVTVV
                     PAPDARGLEFDTVIVVDPDEIEGHSEAGLRDLYVAQTRATQRLITLAIAPRGGAAVAS
                     "
     gene            complement(1193..2764)
                     /locus_tag="VN041_17130"
     CDS             complement(1193..2764)
                     /locus_tag="VN041_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30807.1"
                     /translation="MASLWLDTQREPVPSDAFDPDARYDAVVVGAGLTGLTTALLLAR
                     AGLAVAVLEARSIGAVTTGHSTAKLSLLQGGVLSGIRSRGSRRVLQAYVDANREGRDW
                     MLRYLDEHDVELQRRDAFTYAGSPEGTELLDEEYEACRDAGLDVERVTETGLPFETHG
                     ALRLPDQAQFDPMDVLHALAADLRSRGAVIVEGVRVKDVDTGDPCRVITPLGDVHATT
                     VVLATGTPILDRGLYFAKLKPSRSYAQAYRVPGGGLPHGMYLSIDTPGRSLRTAPVGA
                     EELLLVGGNGHTVGREEDTRRLADDLEDWTRQRFPGAERTHAWSAQDYESMNLVPFVG
                     WMPRTGHRVALATGYNKWGMTNAVAAALSIAADVLGGSIPWAKTLHHRVTLPKDVAHG
                     VAANAQVAGRLASGWVASGLHPLPEDPPAEGEGAVGAYDMEPTAVSTVDGTTCRLSAV
                     CTHLGGIVTWNSAERSWDCPLHGSRFDAQGRLLEGPAVEDLHAIDEPELPGDAHEPVE
                     DPDENADPPDAADAR"
     gene            2901..>3442
                     /locus_tag="VN041_17135"
     CDS             2901..>3442
                     /locus_tag="VN041_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWU30808.1"
                     /translation="MSQEQRALLAIDAGQTGIKTRLIRADGTATDALFSGVLTDRPLL
                     PQLAEVVASAGGSPDTITFGVSGLTDADAEASALLAHPALAGVRRVVVTHDSVTSFLG
                     VLGDRYGAVVAAGTGVVTLGVGPEHSTRVDGWGYIMGDAGSGYWIGRSALDAVMRAYD
                     GRGPATALTAVVQDRWPDLDS"
BASE COUNT          501 a         1256 c         1193 g          492 t
ORIGIN      
        1 tcgacaccgc gcacggagcg gtggtgctgg actcccggcg cgtcgacgcg gcccgccgca
       61 gcgcgctgca gggcggcgtc ggacacaacc gcgcacggga actgttcctg gagtacgtcg
      121 tggacgacct tgtggacgaa ctggagcagc agaccgcgaa ggagatgtcg gatttcgagg
      181 aggagctcaa ggaggtcggt gtcgatctcg accgcatgtt cgcgcgtcag cacggcgagc
      241 cggagccgca cggtctcgaa tcgtcccccg gcggcctgga catcgattgg gatcgcatcc
      301 gcgacgatct gctcgacgac gccggcatcg gtcggatcgt ctccgaggtg tggcccgcgc
      361 ttcgcgcgga ggacgtgctg ctcggcctgc tcgcggatcc tgaggcgctc gcgctcgccg
      421 cgccggagct ctcggacgag cagatcgcct cggtcgtgtc cggggcgggc gcgggatgga
      481 gcagggctga tctcgcggca ctcgacgaag cgcgtgcgct cgtggacgga atccccgagg
      541 tgacctacgg gcacgtcgtc gtcgacgagg cgcagcagct ctccgagatg gagtggcgca
      601 tgctcatgcg ccactgcccc agccgctcga tgaccatcgt cggcgacctc gcccaggccg
      661 gcccgacgac gacgatcacg cggtgggagg aggcgctgga gccgttcgtg gacggtcgat
      721 acgcgcatca cacgctgacc gtcaactatc ggacgaccgc cgagatcctc gacgcgaccc
      781 gcccggtgct cgcccgcatc gccccgtcac agcgtctgtc gaactcgatc cggcacggcg
      841 aagtcccgag ccaccacacc gtcgccgatg tcgatcgcgc gctcggcgac ctggtcgctc
      901 gggtgcgcga cgagcatccc cagggtctga tcgggatcat cgccgcgcca cgccgcatcc
      961 gagcgctgga cgcggggttc gcgggagacg gcgtcacggt cgtgcccgcg cccgacgcac
     1021 gggggctgga gttcgacacg gtgatcgtcg tcgatcccga cgagatcgaa ggccacagcg
     1081 aagccggcct ccgcgatctc tacgtcgcgc agacccgagc gacccagcgc ctgatcactc
     1141 tcgcgatcgc accgcgcggc ggagcagcag tcgcgtcctg aggccgcgag ggtcagcgag
     1201 cgtccgccgc gtcgggagga tccgcgttct cgtccggatc ctccacgggc tcatgcgcgt
     1261 cgccgggcag ttccggttcg tcgatcgcgt gcagatcctc gaccgccggc ccctccagca
     1321 gccggccctg cgcgtcgaag cgggacccgt gcagcggaca gtcccagctg cgctccgcgc
     1381 tgttccaggt gacgatgccg ccgaggtgcg tgcacacggc ggacaggcgg caggtggtgc
     1441 cgtccacggt cgagaccgcg gtgggctcca tgtcgtacgc ccccacggcg ccctcgccct
     1501 cggcgggcgg atcctccggc agcggatgca gaccggaggc cacccagccc gaggcgagcc
     1561 ggcccgccac ctgggcgttc gccgcgacgc cgtgggcgac atccttcggc agagtcaccc
     1621 gatgatgcag cgtcttcgcc cacgggatgc tgccgcccag aacgtccgcg gcgatgctca
     1681 gggccgcggc gacggcgttc gtcatccccc acttgttgta gccggtcgcc agggcgacgc
     1741 gatgccccgt gcgcggcatc cacccgacga acggcacgag gttcatcgac tcgtagtcct
     1801 gcgccgacca cgcgtgcgtg cgctcggcac ccgggaacct ctgccgcgtc cagtcctcca
     1861 ggtcgtcggc gagccgccgg gtgtcctcct cacgaccgac cgtgtgcccg ttgccgccga
     1921 cgagcagcag ctcctctgcg ccgaccgggg ccgtgcggag ggagcggccc ggcgtgtcga
     1981 tggagaggta catgccgtgc gggagtccgc cgcccggaac ccggtacgcc tgcgcgtagg
     2041 agcgcgacgg cttcagcttg gcgaagtaca ggccgcgatc gaggatcggc gtgcccgtgg
     2101 cgagcaccac tgtcgtcgcg tgcacatcac cgagcggcgt gatcacgcga cacgggtcgc
     2161 ccgtgtcgac gtccttcacc cgcacgccct ccacgatcac cgcgccgcgg gagcgcagat
     2221 cggcggccag cgcgtgcagc acgtccatcg ggtcgaactg cgcctgatca ggcagccgca
     2281 gggccccatg cgtctcgaag gggaggccgg tctcggtcac ccgctcgaca tcgagtcccg
     2341 catcccggca cgcctcgtac tcctcatcca gcaactccgt cccctccggt gagccggcat
     2401 aggtgaacgc gtcacggcgc tgcagttcga catcgtgctc gtcgaggtag cgcagcatcc
     2461 agtcgcggcc ctcccggttc gcatcgacgt acgcctgcag cacccgcctc gagccgcggg
     2521 agcggatgcc cgacagcacg ccgccctgca gcagactcag cttcgcggtg ctgtggcccg
     2581 tcgtgaccgc gccgatcgag cgggcctcga gcacggccac ggcgagcccc gctctggcca
     2641 gcagcagtgc ggtggtcagg ccggtgagtc ccgccccgac gacgaccgcg tcgtagcggg
     2701 catccggatc gaatgcatcc gagggaacgg gttcgcgctg ggtgtcgagc cacagtgatg
     2761 ccacaggagc ctccttcacg ggaccggagc tcaagtacac cgcgctccgc gccgaaccca
     2821 tcggggcttg acttcgacgg acatcccacc cagtcgatgt cctacgctgg cacggggccc
     2881 gccacggaga ggacacgtct ttgtcacagg aacagcgcgc gctgctcgcg atcgacgccg
     2941 ggcagaccgg catcaagacc cgcctgatcc gtgccgacgg cacggcgacg gacgcgctgt
     3001 tctccggtgt gctcaccgat cggccactgc tgccgcagct ggccgaggtc gtggcttccg
     3061 ccggcggttc ccccgacacc atcaccttcg gagtctccgg actcacggac gcggatgccg
     3121 aggcatccgc cctgctcgcg catcccgcac tcgcgggcgt gcgccgcgtg gtcgtgacgc
     3181 acgattcggt cacctcgttc ctgggcgtgc tgggcgacag gtacggcgcc gtggtcgcag
     3241 caggcaccgg ggtggtcact ctcggcgtcg gccccgagca ctccacccgc gtcgacggct
     3301 ggggctacat catgggagac gccggcagcg ggtactggat cgggcgctcc gcgctggatg
     3361 ccgtgatgcg cgcgtacgac gggcgcggtc ccgccaccgc gctgaccgcc gtggtgcagg
     3421 accgctggcc ggacctcgac tc
//
LOCUS       DATMSX010000540         9162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_8851,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000540 DATMSX010000000
VERSION     DATMSX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8851"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VN041_17140"
     CDS             complement(<1..222)
                     /locus_tag="VN041_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30809.1"
                     /translation="MSEPATHTPVFVDSAGRRLRWVRAGLWVAVLAAIVYIALAASAL
                     LGGPRIDAPFLPQPITADAPPPAQHVEVPA"
     gene            339..1217
                     /locus_tag="VN041_17145"
     CDS             339..1217
                     /locus_tag="VN041_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HWU30810.1"
                     /translation="MPTTLSRDEWNARARAHEARADALTAAHRERAVRGERHPVWDFL
                     FTYYSYKPAMLRRWHPGAGVVLQDAPERIGWRWYSSADGVAVAPDADLFRAEKPQLAD
                     LVERMLRRTASRPGQFGCFGLHEWAMVYREEEHRHPVPLRLGQAGTDAVVDTHELRCT
                     HFDAFRFFTADAVPRNRRPLTRDSQPDLEQPGCLHAGMDLYRWAVKLGPLIPGELMLD
                     AFELARDIRLLDMEAAPYDLSAWGVRPVRIETADGKAEYVRRQREFAERGTVMRERIL
                     DAWLGTSTGSAAPIGH"
     gene            complement(1211..2377)
                     /locus_tag="VN041_17150"
     CDS             complement(1211..2377)
                     /locus_tag="VN041_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU30811.1"
                     /translation="MRRSAWGTAIAALAVIALVGCAPSTASPQASASRTAAPTPSPSP
                     TPTVDPAKAAVARMSLAQRASSVIMGTVPGTDAAALRAFMSSGPEGSSLGGFILMGSN
                     IAADAAQEKAVTDALVIDPALPPLIAIDQEGGYVSRLPWDTLPGGRRLQSADPAQVQQ
                     VFAQRAALVAATGANLNFGIVADVPANPKSFIAPRALGTDPASSSARVVAAVEGEKGV
                     VLSTLKHFPGHGATPGDSHVSIPSTPMGLDEWRAGPAAPFAAGIGAGAEVLMFGHLAY
                     TAVDAAPASLSPRWHEIARNDLGFTGLMITDDIGMLTSSGVAAYRDPVVDAVTALAAG
                     NDMVLMIAGSDARTAGRMAQAIVTAVQDGTLPAERLQDAAEHVMAQRMALRPVQ"
     gene            complement(2387..3457)
                     /locus_tag="VN041_17155"
     CDS             complement(2387..3457)
                     /locus_tag="VN041_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWU30812.1"
                     /translation="MSILQSVVIGAGQAGLSASYRLKRLGIEHIVLDANDRPGGAWQH
                     RWDALTMQDVHRVAELPGDTPPAHTFERANQAVPRTFGSYERAHDLPVLRPIAVRQVV
                     DEDGILVVRADQGEWRTRTLVNATGTWTKPFLPHYPGMETFLGEQLHTVDYPGPEHFL
                     GKRVLVVGGGASAVQFLGALAPITDTLWVTRRPPVWRTDDFSPEAGAAAVALVEKRVA
                     QRLPPESVVSVTGLMLRPQEREAERLGAYAARREMFQSIEPDGVRWADGSFERVDVIL
                     WATGFRPAIAHLAPLHLRSPLGGIQLDRAGRGTTAVADPRIQLVGYGPSASTIGANRA
                     GRTAARGVQRSLAARETVSVVA"
     gene            3678..4730
                     /locus_tag="VN041_17160"
     CDS             3678..4730
                     /locus_tag="VN041_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019618956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HWU30813.1"
                     /translation="MLDGDLPAWLLWLLLGGIVAVITVSAFGVVTEAEHLAHRLGDPY
                     GTLVLTISIAAIEVILIAAVMLGPGEHTTIARDSVMAVSMIIMNLVIGIALLVGGLRH
                     RRLRANPTGVSLYLGMLIVLLSAAFALPALIGSEGAYDPAQAVPLVLLTVGLYAFFLL
                     RQMGAQRTDFQETADDGDAASSSSSPDAVRAPIGAVLVEHRAELTARTLLLIAMVLPI
                     VLLSHDMATLLDEGLDRLGAPVALSGILIAMIVFLPETITTIRAALGGEMQRVSNLCH
                     GALVSTAGLTIPAVLTIGLLTGQHVLLAESPVNLLLLGMTLLLTMATFLGRRLTALHG
                     AAHLFVFVLYGMSVFS"
     gene            4765..5202
                     /locus_tag="VN041_17165"
     CDS             4765..5202
                     /locus_tag="VN041_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU30814.1"
                     /translation="MAIMRMDNVGIVVDDLDLAVEFFTVLGMELEGRQLIEGEWAGRV
                     VGLSGMRSEIAMMRIPGAPGKLELATYHAPELLGRGVQAIPHTLGFHRVMFAVDDLDD
                     TIARLGPLGGELLGEVVDYRGLVRLCYFRGPGGIVIGLAEDAA"
     gene            complement(5246..5839)
                     /locus_tag="VN041_17170"
     CDS             complement(5246..5839)
                     /locus_tag="VN041_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30815.1"
                     /translation="MTSATFVPGPAPTRLTRLRDLPRRGLVAGGGHVREICGQWGPGG
                     ELHWMLQLVELRRPEGVLHLPPDAAHTLAGIGGPQVGVGPGAAVGLRKERVLHYAGAF
                     LELRRPQLRAAELSRVLVLSSVPARATPLLVFSDLHGAPALPADVRAIVVRSGRVSAG
                     GSTATAMEALVVPDEARDEGAGLGVVADDARVLMVVG"
     gene            complement(5844..7112)
                     /locus_tag="VN041_17175"
     CDS             complement(5844..7112)
                     /locus_tag="VN041_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWU30816.1"
                     /translation="MDQGNTAFVLICAALVLLMTPGLAFFYGGLVKAKSVISMMMMSF
                     GAIGLIGVLWVVFGYAIAFPSGTGLIAPWTIDWSAIGLNSLLEVPKGAAYPPLAFVAF
                     QATFAIITVALVSGAIADRARFGAWMVFAGVWATVVYFPVASWVFNFGLAEDGSFAYG
                     GWVTKGLQDMLGVGAIDFAGGTAVHINAGAAGLALALVLGRRIGFQKGADVPHNPPFV
                     LLGAGLLWFGWFGFNAGSELGADGTASLAFVNTLAAPAAALLSWLVMEKIRNGKPTSV
                     GAASGAVAGLVAITPACAALSPIWAVVLGLLAGAVCQLAIDLKFKLGFDDSLDVVGIH
                     LIGGIIGTLYLGFFANDTGLIYSGSFAQLAAQALAAGAVLVYSFGLALVIGFVVQKTM
                     GFRVKDEDEIAGLDTVVHGEEGYVLIGARV"
     gene            complement(7305..7985)
                     /locus_tag="VN041_17180"
     CDS             complement(7305..7985)
                     /locus_tag="VN041_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30817.1"
                     /translation="MSAVLRAEGLSRTFTSAGGDVVAVREAELAVEPGELVVIAGRSG
                     AGKTTLLMMLGGLDRPTAGRVLVDGVDLADPKTDVAALRGSRIASIFQTAGLIPVLSA
                     AENVEVPLRIRRTEPAERDRRVADALRDVGLEDHARQRPGELSGGQQQRVGIARALVM
                     SPGILLADEPTAQLDSETGGQIMELIARLVHDRGTAAVVATHDAAMFARADRVLELHD
                     GVLRRREG"
     gene            complement(7982..8911)
                     /locus_tag="VN041_17185"
     CDS             complement(7982..8911)
                     /locus_tag="VN041_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30818.1"
                     /translation="MSDDGSIVCEGLVRIFTAQGVEVQALQGLELRMSRGEMVALIGA
                     SGSGKSTLLGILAGLDRPTAGSARVAGHDLVTMRGAERLRYRRQSVGFVWQQSARNLL
                     PYLSARENIAMVHEVAGVVPRMRRAAAGDELLELLGAADVADRHPAEMSGGQRQRVAI
                     AVALANGPRVLLADEPTGELDEQTSGEVLAAMETVNRERGVTTLIVTHDAGVTEHVER
                     TVRIRDGRTSTETLRRTHLDDEGRSVRMAQEYAVLDRAGRMQLPADFVSALELRERVR
                     LTLEPDHVRVAPGHGHPAEGTSDDAPDTREGGA"
     gene            complement(8938..>9162)
                     /locus_tag="VN041_17190"
     CDS             complement(8938..>9162)
                     /locus_tag="VN041_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30819.1"
                     /translation="GVGVATAWLVIPQLDLHGFVGGADQPPVLLGGWWPVLAVIAFSG
                     LSALAVLVATGLASRLGAAEAIRADDERGQ"
BASE COUNT         1315 a         3335 c         3179 g         1333 t
ORIGIN      
        1 ggcggggacc tcgacgtgct gcgcgggagg cggggcgtcg gctgtgatcg gctgcggaag
       61 gaacggggcg tcgatccgcg gaccgcccag cagtgcgctg gccgcgaggg cgatgtagac
      121 gatcgccgcg aggacggcga cccacagccc tgcgcgcacc caccgcaggc gtcggcccgc
      181 ggagtcgacg aacacagggg tgtgcgttgc gggttctgac atgcggggac tccctcgggt
      241 gggcatcacc atggtacgac ggcatcccgg acgctcgctg gacggtgtcc tagggcggac
      301 ccggcgttcc gatcggcgcg gccgcgtatc ctcgtgtggt gcccaccact ctctcccgcg
      361 acgagtggaa cgcgcgcgcc cgcgcgcatg aggcgcgagc ggatgcgctg accgcggcgc
      421 accgcgagcg ggcggtgcgc ggcgagaggc atccggtgtg ggacttcctc ttcacgtact
      481 actcgtacaa gccggcgatg ctgcgtcgct ggcacccggg cgccggagtc gtgctgcagg
      541 acgcaccgga acggatcggg tggcgctggt actcctccgc ggacggcgtc gccgtcgccc
      601 cggacgcaga tctgttccgc gcggagaagc cgcagctcgc ggacctcgtg gagcggatgc
      661 tgcgccgcac cgcctcgcgc cccggccaat tcggatgctt cggcctgcac gagtgggcca
      721 tggtctaccg ggaggaggag caccgccatc cggttcccct tcggctgggc caggccggca
      781 cggatgcggt ggtcgacacg cacgagctgc gctgcacgca cttcgacgcc ttccggttct
      841 tcactgccga cgccgtgccg cgcaaccgcc gcccgctcac gcgcgactcc cagcccgacc
      901 tggagcagcc cggctgcctg catgcgggca tggacctgta ccggtgggcg gtgaagctgg
      961 gcccgctgat ccccggtgag ctcatgctgg acgccttcga gctcgcgcgc gacatccgtc
     1021 tgctcgacat ggaggccgca ccctacgacc tgtccgcctg gggcgtgcgg ccggtgcgca
     1081 tcgagaccgc cgacggcaag gcggagtacg tgcgccggca gcgtgagttc gccgagaggg
     1141 gaacggtcat gcgggagcgg atcctggacg cctggctcgg tacctcgacg ggctcagcgg
     1201 cccccatagg tcactgaacc ggtcgaagcg ccatccgctg ggccatgacg tgctcggcag
     1261 cgtcctggag ccgctccgcg ggcagcgtgc cgtcctgcac cgccgtgacg atcgcctggg
     1321 ccatccgccc ggccgtccgc gcatccgacc ccgcgatcat cagcaccatg tcgttgcccg
     1381 cggcgagcgc ggtgacggca tccaccaccg gatcgcggta ggcggcgacc cccgatgagg
     1441 tgagcattcc gatgtcgtcg gtgatcatga ggcccgtgaa ccccaggtcg ttccgcgcga
     1501 tctcgtgcca gcgcggtgag agcgaggccg gtgcggcatc caccgccgtg tatgcgagat
     1561 gcccgaacat gagcacttcg gcgcctgcgc cgatgccggc cgcgaacggc gccgccggtc
     1621 ccgcccgcca ctcgtcgagg cccatcggtg tcgaggggat gctgacgtgc gagtccccgg
     1681 gtgtcgcccc gtggccgggg aaatgcttca gcgtagagag cacgaccccc ttctccccct
     1741 cgacggcggc cacgactcga gctgaagagg atgccgggtc cgtgccgagc gctcgcggcg
     1801 cgatgaacga cttcgggttc gcgggcacat cggcgacgat gccgaagttg aggttcgccc
     1861 cggtcgcggc gaccagggcc gctcgctgcg cgaacacctg ctgcacctga gccgggtctg
     1921 cggactgcag gcggcgcccg cccggcaggg tgtcccaggg caggcgggag acgtagccgc
     1981 cctcctggtc gatcgcgatc agagggggaa gggcgggatc gatgacgagc gcgtcggtga
     2041 ccgccttctc ctgggccgca tcggcggcga tgttcgatcc catcaggatg aagccgccga
     2101 gggaggagcc ttccggcccg gagctcatga acgcgcgcag tgcggcggcg tcggtgccgg
     2161 gcaccgtgcc catgatcacg ctcgacgccc gctgggcgag gctcatcctc gcgaccgcgg
     2221 ccttcgccgg gtcgactgtc ggggtgggcg acggcgacgg cgtgggcgcg gcggtgcgcg
     2281 aagcggacgc ctggggcgac gccgtcgatg gcgcgcagcc caccagggcg atcacggcga
     2341 gcgcggcgat cgctgttccc caggcggatc tgcgcatgcg tccacgctac gcgacgacgc
     2401 tgaccgtctc ccgggcggcg agactgcgct gcacgcccct ggccgcggtg cggccagcac
     2461 ggttggcgcc gatggtgctc gcggagggcc cgtagccgac cagctgtatg cgcggatcgg
     2521 ccaccgccgt cgtgccccgc cccgcacggt cgagctggat gccgccgagc gggctgcgca
     2581 ggtgcaacgg tgcgagatgc gcgatggcgg gccggaagcc cgtcgcccag aggatcacgt
     2641 ccacgcgttc gaaggagccg tccgcccagc gcacgccgtc gggttcgatg ctctggaaca
     2701 tctcgcggcg ggcggcatag gcgccgagcc gctcggcctc gcgctcctgc gggcggagca
     2761 tgagcccggt gacgctgacc acgctctccg gcggcagccg ctgcgcgacg cgcttctcga
     2821 cgagggcgac ggcggcggcc ccggcctcgg ggctgaagtc gtccgtgcgc cacaccggcg
     2881 ggcgccgggt gacccacagg gtgtcggtga tcggcgccag cgcgccgagg aactgcacag
     2941 ccgaggcgcc gccgcccacc accagcaccc gcttgccgag gaaatgctcg gggcccgggt
     3001 agtcgacggt gtgcagctgc tccccgagga acgtctccat gcccgggtag tgcggcagga
     3061 agggcttggt ccacgtgccg gtcgcgttca cgagggtgcg ggtccgccac tccccctggt
     3121 ccgcacgcac cacgaggatg ccgtcctcgt cgacgacctg gcgcaccgcg atgggccgga
     3181 ggaccggcag gtcgtgagcg cgctcgtagg agccgaaggt gcggggcacc gcctggttcg
     3241 cccgctcgaa agtgtgcgcg ggcggggtgt ccccgggaag ctcggccacc ctgtgcacgt
     3301 cctgcatggt gagggcgtcc cagcggtgct gccacgcgcc gcccgggcgg tcgttcgcgt
     3361 ccagcacgat gtgctcgatg ccgagccgct tcaggcggta cgaggccgac agaccggcct
     3421 ggcccgcccc gatgacgacg ctctggagga tgctcacacc gcttcccaac gtccgcctgc
     3481 gtcgggatgt tcccttggta gaatcggggt gaagcgaccg ccgggacgac ccgcgcggag
     3541 tcatcacagg cggggacggc cctgcggatt cggcatccgc catacgaacc ctgctccgca
     3601 ccgtcctcac cccgaccgcc atcctccggc tggtcctcgg ctggggctcc ttcgtcctgc
     3661 tgctcgcgct cgcccccgtg ctggacggcg acctgcccgc ctggctgctg tggctgctgc
     3721 tcggcggcat cgtcgccgtg atcacggtga gcgcgttcgg cgtcgtcacc gaggcggagc
     3781 atctcgcgca ccggctcggc gatccgtacg gaaccctcgt gctgacgatc tcgatcgccg
     3841 cgatcgaggt gatcctcatc gccgcggtga tgctcggccc gggcgagcac accacgatcg
     3901 cccgcgactc cgtgatggcc gtgtccatga tcatcatgaa tctcgtgatc ggcatcgcgc
     3961 tgctggtggg tggcctgagg catcggcgtc tccgcgcgaa ccccaccggc gtctcgctgt
     4021 atctcgggat gctgatcgtg ctgctctccg cggcgttcgc gctgcccgcg ctgatcggga
     4081 gtgagggcgc atacgacccg gctcaggcgg tccctctcgt cctgctgacg gtgggtctgt
     4141 acgccttctt cctgctgcgg cagatgggcg cgcagcggac ggacttccag gagacggcgg
     4201 atgacggtga tgcggcctcc tcttcttcct cccccgatgc ggtgcgggct ccgatcggcg
     4261 cagtgctcgt cgagcatcgt gccgagctga ccgcccgcac cctgctgctc atcgcgatgg
     4321 tgctgccgat cgtgctgctc tcgcacgaca tggcgacgct gctggatgag gggctggaca
     4381 ggctgggagc acccgtcgca ctgtcgggca tcctcatcgc gatgatcgtg ttcctgcccg
     4441 agacgatcac cacgatccgg gctgctctcg gcggtgagat gcaacgcgtc agcaacctgt
     4501 gccacggggc cctcgtctcc acggcggggc tgaccatccc cgccgtgctc acgatcgggc
     4561 tgctcaccgg tcagcacgtg ctgctggccg agtcgccggt gaacctgctg ctgctcggca
     4621 tgacgctgct gctgaccatg gccacgttcc tgggtcggcg tctcaccgcg ctgcacggcg
     4681 cggctcatct gttcgtgttc gtgctgtacg ggatgagcgt gttcagctga tggctccgcg
     4741 cgcgcaccgg agtacggtgg ccgcatggcg atcatgcgca tggacaatgt gggaatcgtg
     4801 gtggacgacc tcgacctcgc cgtcgagttc ttcaccgtgc tcgggatgga gctcgaggga
     4861 cggcagctga tagagggcga atgggccgga cgggtcgtcg ggctgagcgg gatgcgctcg
     4921 gagatcgcca tgatgcgcat ccccggtgct ccgggaaagc tggagctcgc gacctatcac
     4981 gcgccggaac tgctcggccg gggtgtgcag gcgatccccc acacgctcgg gttccatcgg
     5041 gtgatgttcg ccgtcgatga tctcgacgac acgatcgcgc gcctcggccc gctcggcggc
     5101 gaacttctgg gcgaggtcgt cgactaccgc ggactcgtcc gcctgtgcta cttccgcgga
     5161 ccgggcggaa tcgtcatcgg cctcgcggag gacgccgcct gacggtgagc cgagcagatc
     5221 cgctggactc ggatccgctc ggctctcagc cgacgaccat cagcacccgg gcatcgtcgg
     5281 cgacgacccc gagccctgcg ccctcgtccc gtgcctcgtc cgggacgacc agcgcctcca
     5341 tcgccgtcgc ggtgctgcca cctgcgctga ccctccccga gcgcaccacg atggctcgca
     5401 cgtcggcggg gagcgccggc gcgccgtgca gatccgagaa gaccagcaag ggtgtcgcac
     5461 gcgccggcac cgaggagagc acgagcacac ggctcagctc tgcggcccgc agctgcgggc
     5521 gccgcagttc gaggaacgcg cccgcgtagt gcagcacgcg ctctttgcgg agccccaccg
     5581 cggctcccgg cccgacgccc acctgcggcc cgccgatgcc ggcgagcgtg tgcgcggcgt
     5641 ccggcgggag atggagcacg ccctccggac gccgcagctc gaccagctgc agcatccagt
     5701 gcagttcccc gcccggcccc cactgcccgc agatctcacg gacgtgtccc cctccggcca
     5761 cgagccctcg ccggggcagg tcccgcaggc gcgtgagcct ggtcggggcc ggccccggaa
     5821 cgaaggttgc actcgtcatc ggatcagacc cgcgctccga tgaggacgta gccctcttcg
     5881 ccgtggacca ccgtgtcgag accggcgatc tcgtcctcgt ccttgacgcg gaagcccatg
     5941 gtcttctgga ccacgaagcc gatcacgagt gccagcccga acgagtacac cagcaccgct
     6001 ccggcggcga gcgcctgagc cgcgagctgc gcgaagctgc cgctgtagat gagtcccgtg
     6061 tcgtttgcga agaagccgag gtacagcgtc ccgatgatgc cgccgatgag gtggatgccc
     6121 accacgtcga gcgagtcgtc gaagccgagc ttgaacttga ggtcgatcgc gagctggcag
     6181 accgcgccgg cgagcagacc gagcacgacg gcccagatcg gcgagagggc ggcgcacgcc
     6241 ggggtgatcg cgacgagacc ggcgaccgca ccggatgcgg cccccaccga cgtcggcttg
     6301 ccgttgcgga tcttctccat cacgagccag ctgagcagcg ctgcggcggg ggcggcgagg
     6361 gtgttcacga acgcgaggga ggcggttcca tcggcgccga gctcggagcc cgcgttgaac
     6421 ccgaaccagc cgaaccacag caggcccgca ccgaggagca cgaacggcgg gttgtgcggc
     6481 acgtcggcgc ccttctggaa gccgatgcga cggccgagca ccagggcgag cgcgagtcct
     6541 gcggctcccg cgttgatgtg gaccgctgtt cctcccgcga agtcgatcgc gccgacgccg
     6601 agcatgtcct gcagaccctt ggtgacccag ccgccgtagg cgaagctgcc gtcctcggcc
     6661 aggccgaagt tgaagaccca gctcgcgacc gggaagtaga cgacggtcgc ccagacgccg
     6721 gcgaagacca tccaggcgcc gaaccgggcg cggtccgcga tcgcgcccga cacgagggcg
     6781 acggtgatga tcgcgaacgt cgcctggaat gcgacgaagg cgagcggcgg gtaggcggcg
     6841 cccttcggaa cctcgagcag gctgttcagc ccgatcgccg accagtcgat cgtccacggg
     6901 gcgatcagcc ccgtaccgct cgggaacgcg atcgcatagc cgaagacgac ccacagcacg
     6961 ccgatcagtc cgatcgcgcc gaagctcatc atcatcatgc tgatcacgct cttggccttg
     7021 acgaggccgc cgtagaagaa cgccagacct ggcgtcatca gcagcacgag ggctgcgcat
     7081 atcaaaacga aggcggtgtt cccctgatcc atgggtccgc tcctctctca gtgagaacgc
     7141 tcctgcagcg tcgggtgtgg aaagactccc accggcgcgt ttcgctccga cgcgcactgt
     7201 gtttccgggg cgtttcggcg cagacccggc gcgtgaacac ctcgttacag ggaccggatc
     7261 agcggcatcc ccgcgctctc gaccgctcga cgcggccgtg gcgttcagcc ctcacgtcgg
     7321 cgcagcaccc cgtcgtgcag ttccagcacg cggtcggccc gggcgaacat cgccgcgtca
     7381 tgagtggcca cgacggctgc ggtgccccga tcgtgcacca gccgggcgat gagctccatg
     7441 atctgcccac cggtctcgct gtccagctgc gcggtgggct catcggcgag caggatgccc
     7501 ggagacatga ccagtgcccg tgcgatgccg actcgctgct gctgccctcc cgacagctcc
     7561 cccggccgct gcctggcgtg atcctcaagg ccgacatcgc gcagggcatc cgcgacccgg
     7621 cgatcccgct cggccggctc ggtgcggcgg atgcgcagcg gcacctccac gttctcggcg
     7681 gccgagagca ccgggatgag gcccgcggtc tggaagatcg acgcgatgcg cgatccccgc
     7741 agggcggcga cgtccgtctt cgggtcagcg agatcgacgc cgtcgacgag cacccggccc
     7801 gctgtgggac ggtcgagtcc gcccagcatc atgagcagcg tcgtcttccc cgctccggat
     7861 cgcccggcga tcaccacgag ctcccccggt tcgacggcca gctcggcctc gcgcaccgcc
     7921 acgacatctc ccccggcact cgtgaacgtc ctcgacagcc cctcggcccg cagcacagcg
     7981 ctcacgctcc gccctcccgc gtatcgggcg catcatccga tgtgccctcc gcgggatgcc
     8041 cgtgcccggg cgcgacccgt acgtgatcgg gctcgagagt gagcctcacc cgctcacgca
     8101 gctcgagcgc gctgacgaag tcggccggca gctgcatgcg cccggcgcga tcgagcacgg
     8161 cgtactcctg cgccatccgc accgaccgtc cctcgtcgtc caggtgcgtg cgacgcagcg
     8221 tctccgtcga ggtgcgcccg tcgcggatcc ggaccgtgcg ctcgacgtgc tcggtcaccc
     8281 ccgcatcgtg cgtgacgatg agggtcgtca caccgcgctc gcggttcacg gtctccatgg
     8341 cggccagcac ctcgccgctg gtctgctcgt cgagctcccc ggtcggctca tccgccagca
     8401 gcacgcgagg cccgttcgcg agggcgaccg cgatcgccac ccgctgccgc tgtccgccgg
     8461 acatctcagc gggatgacga tcggcgacgt cggcggcgcc cagcagctcc agcagctcgt
     8521 ccccggcggc ggcacgccgc atacgcggca cgacgccggc gacctcgtgc accatcgcga
     8581 tgttctcccg cgcggacagg tagggcagca gattgcgcgc cgactgctgc cacacgaagc
     8641 cgacactctg gcggcggtag cgcagccgct cggcacccct catcgtcacc aggtcgtggc
     8701 ccgccacccg ggcggacccc gccgtcggcc ggtcgagacc cgccaggatg cccagcagcg
     8761 tgctctttcc cgatccggac gcaccgatga gcgcaaccat ctccccgcgc gacatccgca
     8821 gctccagccc ctgcagcgcc tgcacctcca cgccctgcgc cgtgaagatg cgcaccagtc
     8881 cctcgcatac gatcgagccg tcgtccgaca tgtggtccat cctctctcct gcgtgcatca
     8941 ctgcccccgc tcgtcgtcgg cgcggatcgc ctcggccgca ccgagtcgcg aggcgagtcc
     9001 cgtggccacc agcacggcaa gcgccgacag accggagaag gcgatcacgg cgagcaccgg
     9061 ccaccagcct ccgagcagga ccggcggctg gtccgcgccg cccacgaacc cgtgcagatc
     9121 cagctgcggg atcaccagcc aggccgtggc gactccgacg cc
//
LOCUS       DATMSX010000541         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_131843,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000541 DATMSX010000000
VERSION     DATMSX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131843"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VN041_17195"
     CDS             complement(<1..1813)
                     /locus_tag="VN041_17195"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWU30820.1"
                     /translation="MHADDTRTLPPLGEDEWPEQTDVLIMGGGPVGLSCALLLAQRGI
                     DVLLVDRRDFVFRFPRAHLLNMRTMEAFSDMGVADDIYAATPQHDRWRKAVWYTTALG
                     DGPFDGMKIGEVVAWGGGDDAERYASAGPRRHANMPQIRLDPLLHRHALADAPGRIRK
                     LQEVVAIEQDADSITATVQGVETVEQHRIRAKYLIAADGGRDSGRLLGIEQEGRADLR
                     EVVNYHVKMDLSEWAEEDALLAHFMHPAGGARRRGTIQAIGPEHYDRHSEEWLVSVAA
                     WMVEGDRDDEAVLFDSIRRMLGLPDDHDIEMISTTHWTYNGLIAERFRSGRAFLAGDA
                     GHQHPPTGGLGVNGGIQDAHNLCWKLAAVLHGQAPDSLLDSYDAERRPTVAWYTAHSL
                     ENANRHPPIALALGFGEDEAAGFRSLERYVSDTPEGERMRQEVEAAVQHNAYDYGQLA
                     VECGYHYWAGAFVPDGTPLPGDQSDPITFHSTSRPGHHLPHAWLAPSAGESGRRAVHD
                     LVRAEGLTLLTSPTAAPRWKAAVSAVGGAMPVTVVPVFDGDGSWASVRQITESGALLV
                     RPDRKVAWRATHLPDDPESAFRDALQTVVCGGDIPADD"
     gene            complement(1872..>2886)
                     /locus_tag="VN041_17200"
     CDS             complement(1872..>2886)
                     /locus_tag="VN041_17200"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWU30821.1"
                     /translation="RLAERAGAPVLVESRRDLERWSFRTDHPAFAGLFEPSAPLLADC
                     DLLVLAGMPTPLEFSSERPSVPPEVPILHVAEDAAEPGRRHRTACAVVGDTRLSLELL
                     AEGFDAPASGPEADPTASAARAAWLATVLDHSAGQRRRWEDEVPLTAEGAAFSAASAM
                     RVLADTVGGEPLLVLDAVTSTLPLLRFLTRTRRDTLFATASGSLGWGMGAALGVALAR
                     EERTLGVVGDGVVQFGVPALWTAVRSHLPVTFVAVNNGRYQAVVSGLRQSGGAARQHD
                     DYPLTDISGVDIAALAASFGIPSVVVDGASSLRAELRAHLALDETDGPRLIEIRVNDD
                     LWA"
BASE COUNT          391 a         1020 c         1058 g          417 t
ORIGIN      
        1 ggtcgtcggc ggggatgtcg ccgccgcaca cgaccgtctg cagcgcgtcg cggaacgccg
       61 actccggatc gtccgggagg tgcgtcgccc gccaggcgac cttcctgtcg ggtcgcacga
      121 gcagggcgcc gctctccgtg atctgacgga cggatgccca ggagccgtcg ccgtcgaaca
      181 cggggacgac cgtgaccggc atcgcaccgc ccacggcgct caccgcggcc ttccaccgcg
      241 gcgcggcggt cggagaggtc agcagcgtca gtccctccgc cctcacgagg tcgtgcacgg
      301 ctcgccggcc gctctcaccg gcggacggcg cgagccaggc gtgcggcaga tgatgcccgg
      361 gacgcgaggt ggagtggaag gtgatcgggt cggactggtc accgggcagc ggggtcccgt
      421 ccggcacgaa ggcgccggcc cagtagtggt acccgcactc gacggcgagc tggccgtagt
      481 cgtaggcatt gtgctgcacg gccgcctcca cctcctggcg catgcgctcg ccctcagggg
      541 tgtccgagac gtacctctcc aggctgcgga agcccgcggc ctcgtcctcg ccgaagccca
      601 gggcgagggc gatgggaggg tgccggttcg cattctcgag cgagtgcgcc gtgtaccagg
      661 cgacggtcgg gcggcgctcg gcgtcatagc tgtcgagcag cgagtcgggg gcctggccgt
      721 gcagcacggc ggcgagcttc cagcacaggt tgtgggcgtc ctggatgccg ccgttgacgc
      781 cgagccctcc tgtcggcgga tgctgatgtc cggcgtcgcc tgccaggaac gcccggcccg
      841 agcggaagcg ctccgcgatc aggccgttgt acgtccagtg ggtcgtggag atcatctcga
      901 tgtcgtggtc gtccggcagt cccagcatcc gccggatcga gtcgaagagc acggcttcgt
      961 cgtcacggtc gccctccacc atccaggcgg cgacggagac cagccattcc tccgagtgcc
     1021 ggtcgtagtg ctcgggcccg atggcctgga tcgtgccgcg acggcgcgcg ccgccggcgg
     1081 gatgcatgaa gtgcgccagc agcgcgtcct cctccgccca ttccgacagg tccatcttca
     1141 cgtggtagtt gacgacctcg cgcagatcgg cgcggccctc ctgctcgatg ccgagcaggc
     1201 gcccgctgtc ccggccgccg tcggcggcga tcagatactt cgcgcggatg cggtgctgct
     1261 caaccgtctc gacgccctgg acggtcgcgg tgatcgagtc cgcgtcctgc tcgatggcca
     1321 cgacctcctg cagcttgcgg atgcgcccgg gcgcgtcggc gagcgcgtgc cggtgcagca
     1381 gcggatccag gcggatctgg ggcatgttgg cgtgccggcg cgggccggcc gatgcgtagc
     1441 gctcggcatc gtcgccgccg ccccaggcca ccacctcgcc gatcttcatg ccgtcgaacg
     1501 gaccgtcgcc gagcgccgtg gtgtaccaga cggccttgcg ccagcgatcg tgctgcggtg
     1561 tcgccgcgta gatgtcgtcg gcgacgccca tgtcgctgaa cgcctccatg gtccgcatgt
     1621 tcagcaggtg cgcccgcggg aaccggaaca cgaagtcccg cctgtccacg agcagcacgt
     1681 cgatgccgcg ctgcgcgagg agcagggcgc acgagagtcc gaccgggccg ccgcccatga
     1741 tcaggacgtc ggtctgctcg ggccattcgt cctcgccgag gggcgggagg gttcgggtgt
     1801 cgtcggcatg catgcgctgc tccttctctg tcgtctcgga cgtctctgtc gtctcggcat
     1861 cgtttccggg ctcacgccca gaggtcgtcg ttcacgcgga tctcgatcag ccgcggtccg
     1921 tcggtctcgt cgagcgccag gtgcgcccgc agctcggccc gcagcgagga ggcaccgtcc
     1981 acgaccacgg aggggatgcc gaaggacgcg gcgagcgcgg cgatgtcgac accgctgatg
     2041 tcggtgagcg ggtagtcgtc gtgctgccgg gctgcgccgc cgctctggcg gaggccggag
     2101 accaccgcct gatagcggcc gttgttcacg gcgacgaacg tcaccggcag gtgcgagcgc
     2161 accgcggtcc agagcgcagg cactccgaac tgcaccacac cgtctccgac gaccccgagc
     2221 gtgcgctcct ctcgggcgag ggcgacgccg agggcggctc ccatccccca gccgagcgag
     2281 ccgctggccg tcgcgaacag cgtgtcgcgc cgggtgcggg tgaggaaccg gagaagcggc
     2341 agcgtggagg tcacggcatc caggacgagc agtggttcgc cgccgaccgt gtcggcaagc
     2401 acccgcatcg ccgaggcggc gctgaacgcc gcgccctccg ccgtcagcgg cacctcgtcc
     2461 tcccatcgtc ggcgctgtcc ggcggaatgg tcgagaacgg tggccagcca ggccgcccgt
     2521 gcagcggatg ccgtcgggtc ggcttccggg ccggatgccg gtgcgtcgaa gccctcggcg
     2581 agcagctcga gcgagagccg ggtgtcgccg acgacggcac aggcggtgcg atgccttctg
     2641 ccgggttcgg cggcatcctc ggcgacgtgg aggatcggca cctccggcgg cacgctgggg
     2701 cgctcggacg agaactccag cggcgtgggc atccctgcca gcaccagcag atcgcagtcg
     2761 gccaggagcg gggcggacgg ctcgaagagc cccgcgaacg cggggtggtc cgtgcggaag
     2821 gaccacctct ccagatcgcg ccgggactcc accagcaccg gggcgccggc gcgctcggcc
     2881 agtcgc
//
LOCUS       DATMSX010000542         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_131873,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000542 DATMSX010000000
VERSION     DATMSX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131873"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VN041_17205"
     CDS             complement(<1..658)
                     /locus_tag="VN041_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30822.1"
                     /translation="MSATPQLPSDLLLWRARQRSGDPLPWDDLGIRFLEDDDLPALLD
                     TSHLDEDDLADPGIRANVAAMAETNALIVWVAEEEDGAVFGYWAGPDGPPVHDGFADA
                     MVVTLDTEGQYMALPGRNITEALCAHASDFDDDAFVALAVRARELAAETDAATAADLI
                     EADALAALPTRAIEGPGRFRDALFTARRAQEEDHGEGRDPKATPHAPIPTPSLSELPP
                     D"
     gene            complement(697..769)
                     /locus_tag="VN041_17210"
     tRNA            complement(697..769)
                     /locus_tag="VN041_17210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(734..736),aa:Leu,seq:taa)
     gene            873..2126
                     /locus_tag="VN041_17215"
     CDS             873..2126
                     /locus_tag="VN041_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HWU30823.1"
                     /translation="MLDLTAELSPTADEGRAARDLAWQDPARYWRGLTEATAHLPAPV
                     AVIERDALRHNAMDLLVRAGGIPIRVATKSVRVRSVLDAVLAIPGFRGILSFTLAEAL
                     WLAEEHDDIVLGYPTVDRAGLATLLSDERLASRITLMIDDLGHLDLIDSIAAPGRRPE
                     VRVAIDVDASFKAPGLGHLGVRRSPLFTAAEVAGFAREVVRRPGFRLVGLQMYEAQIA
                     GQGDAAGPDAPVIRAMQSRSRAELRRRRAEIADAMLSVAPLEFLNGGGTGSLEFTGSD
                     ESLTEATAGSGLFAGRLFDGYRSFRPAPATGFAFDVVRRPAPDIATVLGGGWIASGPA
                     VESRQPEPVWPPRLRTLPREAAGEVQTPLQGEAARELKIGDRVWFRHAKSGEPAERIL
                     SYHLVAGGSVVDELPTYRGEGKAFL"
     gene            2123..3436
                     /locus_tag="VN041_17220"
     CDS             2123..3436
                     /locus_tag="VN041_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HWU30824.1"
                     /translation="MTRPGGTWQNWGRSASVRPVRVERPRSPEGVQRAVKAAASQGLT
                     VKAIGAGHSFTGIAVAPGVLLELDDMQGLVSADAATGRVTLLAGTRLHRIPSLLRPYG
                     LAMENLGDIDRQSIAGAISTGTHGTGTGFGGIATQVRGLTLITADGEFLRIDETHDSE
                     LLAGAVVGLGALGVVVEVTLQCVPAFVLHAVDAPAPLDETLETVHEQAAAADHLEFYW
                     HPHTDVALKKTQTRLPESAKRNPIPPMQHWLDETVLTNGVFGAFCAVSRVVPAIIPPF
                     NRLAVKLTGDGEYSDESHRVLIHHRNVRFREMEYAIPAGNLVPAFEEVRALVADRGWR
                     IEFPVEVRFAASDDLWMSTASGRDSAYIAVHRYWRTDPRPYFDAVEQIMLRYGGRPHW
                     GKLHSLDAEQLRERYPRFDDFIALRDRLDPKRVFGNRHLERILGA"
     gene            3509..4075
                     /locus_tag="VN041_17225"
     CDS             3509..4075
                     /locus_tag="VN041_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWU30825.1"
                     /translation="MEWLWPVLIIVGIVVLIGIYLWSTYNSLVQLNVRVDEAWSGITV
                     QLKRRADLIPNLIETVRGYAAHEKSVFEGVTRARAETLSATGPAEAGVAEGHLQQALR
                     SLFAVAEAYPQLQASQNYLQLQHSLVDTEDKIQAARRFYNGGVRELNTKIKVFPNNMF
                     ARGLGFTEREFFEVADGNAISEPPRVQF"
BASE COUNT          626 a         1514 c         1447 g          636 t
ORIGIN      
        1 ggtccggcgg gagctccgag agcgagggcg tgggaatcgg cgcgtgcggg gtggccttcg
       61 gatcgcgacc ctcgccatgg tcctcctcct gcgctcgccg tgcggtgaag agggcgtccc
      121 ggaagcggcc agggccctcg atggcgcgcg tcgggagcgc ggcgagtgca tccgcttcga
      181 tcaggtccgc cgcggtcgca gcgtcggtct ccgcggcgag ttcccgcgct cggaccgcca
      241 gcgcgacgaa cgcgtcgtcg tcgaaatccg acgcgtgggc gcacagagcc tcggtgatgt
      301 tgcgtcctgg cagcgccatg tactggccct cggtgtcgag tgtgaccacc atggcgtcgg
      361 cgaacccgtc gtggaccggc ggcccgtcgg ggccggccca gtacccgaag accgcaccgt
      421 cttcttcttc ggccacccag acgatcaggg cgttcgtctc cgccatcgcg gcgacgttcg
      481 cgcggatgcc gggatcggca aggtcgtcct cgtccaggtg cgaggtgtcg agcagggccg
      541 gaaggtcgtc gtcctcgagg aaccggatgc ccaggtcatc ccaggggagt ggatcaccgg
      601 aacgctgacg cgcccgccac aggagaaggt cggacggcag ctgcggcgtc gcactcatga
      661 cggtcatcgt agcggtgcgg gaaccagcct tcttcgtgcc cccaccggga ttcgaacccg
      721 gactgaagcg tgtttaagac gcctgcctct gccgttgggc tatgggggcc cgcgccgcaa
      781 gcctagccgc gcccgcgcac ggagcgggat cagtattgaa ccgggtgtca acaatggtga
      841 ttccgggttc cggaacgtcg tagggtgggg gagtgctcga cctcaccgcc gaactgagcc
      901 ccaccgccga cgagggccgc gcggcccgcg acctcgcctg gcaggatcct gcgcgctact
      961 ggcgcggcct cacggaggcc acagcgcacc ttcccgcgcc cgtcgcggtg atcgagcggg
     1021 atgccctccg gcacaacgcg atggacctgc tggtgcgcgc cggcggcatc cccatccgcg
     1081 tcgccaccaa gtcggtgcgg gtgcgctcgg tgctggacgc cgtgctcgcg ataccgggat
     1141 tccgaggcat cctgtccttc accctcgccg aggccctctg gctcgccgag gaacacgacg
     1201 acatcgtgct cggctacccg acggtggatc gcgccgggct ggcgacgctg ctctccgacg
     1261 agaggctcgc atcgcgcatc accttgatga tcgacgacct ggggcacctc gacctgatcg
     1321 actccatcgc cgcgcccggg aggcgacccg aggtgcgcgt cgcgatcgac gtggacgcct
     1381 cgttcaaagc cccggggctc ggccacctcg gcgtccggcg ctcgccgctg ttcaccgcgg
     1441 cggaggtggc gggcttcgcc cgcgaggtcg tgcgccggcc cggcttccgc ctggtcgggc
     1501 tgcagatgta cgaggcgcag atcgctggtc agggggacgc ggccggcccc gacgcgcccg
     1561 tgatcagggc catgcagtcc cgctcccggg cggagctgcg ccgccgtcgc gcggagatcg
     1621 cggatgccat gctcagcgtc gctccgctcg agttcctcaa cggcggcggc accggatccc
     1681 tggaattcac cggtagcgac gagtctctga ccgaggcgac agccgggagc gggctgttcg
     1741 ccggccggct gttcgacggc taccggtcct tccggcctgc gccggccacc ggcttcgcct
     1801 tcgacgtcgt gcgcagaccc gcccccgaca tcgccaccgt cctcggcggc ggatggatcg
     1861 cctccggacc cgcggtggag tcccgccagc cggaacccgt ctggccgccg cgcctgcgca
     1921 cgctgccgcg cgaagccgcc ggagaggtgc agacccccct ccagggcgaa gcggcgcgcg
     1981 agctgaagat cggcgaccgg gtctggttcc ggcacgccaa gagcggcgaa cccgcagagc
     2041 ggatcctgag ctaccacctc gtcgccggcg gctccgtcgt cgacgaactg cccacctatc
     2101 gcggtgaggg gaaggccttc ctatgacgcg tcccggcgga acctggcaga actgggggcg
     2161 ctccgcatcc gtgcgccccg tgcgggtgga acgcccgcgc tcgcccgagg gcgtgcagcg
     2221 cgcggtcaag gcggcggcat cccagggcct cacggtcaag gcgatcggcg caggccacag
     2281 cttcaccggc atcgcggtcg ctccgggcgt gctgctggaa ctggacgaca tgcagggcct
     2341 cgtctcggcc gacgcggcca ccggacgtgt cacgctgctc gcgggcaccc ggctgcaccg
     2401 catcccgtcg ctgctgcgcc cctacggcct cgccatggag aacctcggag acatcgaccg
     2461 gcagtccatc gccggcgcga tctccaccgg cacgcacggc accggcacgg gattcggcgg
     2521 gatcgccacg caggtgcgcg gcctgactct catcacggcc gacggcgagt tcctgcgcat
     2581 cgacgagacc cacgacagcg agctgctggc cggtgccgtc gtcggcctcg gggcgctcgg
     2641 cgtggtcgtg gaggtgaccc tgcagtgcgt gccggcgttc gtgctgcacg cggtggacgc
     2701 gcccgcgccg ctcgacgaga cgctcgagac cgtgcatgag caggccgccg cggccgacca
     2761 cctcgagttc tactggcatc cgcacacgga cgtggcgctg aagaagacgc agacacgact
     2821 gccggaatcc gcaaagcgga atccgatccc tccgatgcag cactggctcg acgagaccgt
     2881 gctgaccaac ggcgtcttcg gcgccttctg cgcggtcagc cgcgtcgtcc cggcgatcat
     2941 cccgccgttc aaccggctcg cggtcaagct gaccggtgac ggcgagtact ccgatgagtc
     3001 gcaccgtgtg ctcatccacc accgcaacgt gcgattccgc gagatggagt atgcgatccc
     3061 ggccgggaac ctcgttcccg cgttcgagga ggtgcgggca ctcgtcgccg atcgcggttg
     3121 gcggatcgag ttccccgtcg aggtgcgctt cgcggcatcc gatgatctgt ggatgtccac
     3181 cgcctcgggt cgtgacagtg cgtacatcgc cgtgcaccgc tactggcgca ccgatccgcg
     3241 cccctacttc gacgccgtcg agcagatcat gctccgttac ggcggacgcc cgcactgggg
     3301 caagctgcac agcctggatg ccgagcagct tcgggagcgg tacccgcgct tcgacgactt
     3361 catcgccctg cgcgaccggc tcgacccgaa gcgcgtgttc ggcaacaggc atctcgagcg
     3421 cattctcgga gcctgagaac cgcctgtcgt ccagccgatg cgcagagtcg cgcacatagg
     3481 atggatcaag atccaagagg gggcatccat ggaatggctg tggccggtac tgatcatcgt
     3541 gggcatcgtg gtgctcatcg ggatctatct gtggtcgacc tacaactcgc tcgtgcagct
     3601 gaacgtgcgc gtggacgagg cgtggagcgg catcaccgtg cagctcaagc gccgagccga
     3661 cctcatcccg aacctcatcg agaccgtgcg cggctacgct gcccatgaga agtccgtgtt
     3721 cgagggcgtc acccgggcac gtgctgagac gctctccgcg acgggccccg ctgaggcggg
     3781 agtcgccgag ggacatctcc agcaggcgct gcgcagtctc ttcgcggtgg ccgaggccta
     3841 cccgcagctt caggccagcc agaactacct gcagctgcag cactcgctgg tcgacaccga
     3901 ggacaagatc caggcggccc gtcgcttcta caacggcggc gtgcgcgagc tgaacaccaa
     3961 gatcaaggtg ttccccaaca acatgttcgc ccgcggcctc ggcttcaccg agcgcgagtt
     4021 cttcgaggtc gccgacggga acgcgatctc agaaccaccc cgcgtgcagt tctgacggaa
     4081 aggccccgct ccggcggggc ctttctcatt cccgcgggtg cgcgtgacgg tcgaggggcg
     4141 cctcgccgcc gtgcagcgcc cgcgccaccg catccgtcgc gtcaccccac cccgacacgc
     4201 tgatgcgctc caggccctgc cag
//
LOCUS       DATMSX010000543         4316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_150144,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000543 DATMSX010000000
VERSION     DATMSX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150144"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VN041_17230"
     CDS             complement(<1..557)
                     /locus_tag="VN041_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30826.1"
                     /translation="MTDTVSAIQDFIDPLLSDRFKGYPRTQPPRLRSEVGAAGWNVLA
                     GDLPLPLAVLKREALEHNLQWMQSRVREWGIDLAPHGKTTMSPQLFQRQLDAGAWGLT
                     FATVTQLAVGVAAGARRTLIANQVVSDEDLAGIQMLLAQHADLRVVFLVDSTAQLALV
                     EDWAKRNPASVPFDVMLEIGVQGART"
     gene            complement(554..1564)
                     /locus_tag="VN041_17235"
     CDS             complement(554..1564)
                     /locus_tag="VN041_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30827.1"
                     /translation="MTAVSVSSSPSSPPLIEVRGLKKYFGSSERPVRAVDDVSFAIHP
                     GETLGLVGESGSGKSTIGRTVLRLVERTEGQVLYRGDDIGGLSGERMRKLRSKLQIIF
                     QDPYASLNPKMRISAILGEALSTHGLHKGAAARDKRIAELLETVGLRPEHASRFPHEF
                     SGGQRQRIGVARALAVEPEFIVADEPLSALDVSIQSQVINLLADLRERLGLTMLFISH
                     DLDVVEYLCDRVVVLYLGKVMEVATTEELFANPSHPYTRALLAASPKPDPEVATERIA
                     LQGDIPSPISPPSGCVFRTRCPHAIEACAQTVPSLDEISPAHYSACIRKELLSNTAVA
                     RP"
     gene            complement(1561..2607)
                     /locus_tag="VN041_17240"
     CDS             complement(1561..2607)
                     /locus_tag="VN041_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30828.1"
                     /translation="MTNANTTSQSARLTVTNLSTSFPTEDGLIRSVADVSFAIQPGKT
                     TALVGESGSGKSVTSLTLMRLLPKTANAQVSGSACFVTREGKTLDLLQIGGPEMRSLR
                     GNQLSMIFQEPMTSLNPVFTIGEQIAESVRLHKGLDRKAALAHALRMLELVEIPAAAQ
                     RIHEYPHQLSGGMRQRVMIALAMACDPTLLIADEPTTALDVTIQAQILQLMRRLQAET
                     GMSILFITHNLGVVAHHADDVVVLYSGRVVEQAPVRPLFAQPEHPYTQGLLACLPGKA
                     RVPGQPKPKRLFAIRGQVSSPLAPPPGCAFEPRCDQALASCKDAMPPLIDIRQDRQAR
                     CVRVQPDAQLARVA"
     gene            complement(2607..3440)
                     /locus_tag="VN041_17245"
     CDS             complement(2607..3440)
                     /locus_tag="VN041_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30829.1"
                     /translation="MPRMLRQLMYRRIVMVSALVLLVIAVLAVGAPLFASADPNDTAV
                     LQRLKAPSAEHLLGTDELGRDMYARIVHGARYSLAIAALTALGSVIAGTVLGLMAGFF
                     RRLDAPLMRVVDAMMSFPDILLAIALVAILGPSLMNTVLALVLVYTPRVARVVRASTL
                     VVRELLFVEAVRALGVRTSRILWRHILPNLMSPILVQVSFIFAYAILAEAGLSFLGVG
                     VPPEIPTWGTMVAGSQQYAHQAFWVVLFPGLAIIFTALSLQLLGDGVRDLLDPKLKKT
                     S"
     gene            complement(3450..>4316)
                     /locus_tag="VN041_17250"
     CDS             complement(3450..>4316)
                     /locus_tag="VN041_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30830.1"
                     /translation="LTRAASGDPISVLLGDQATAADIARVQKEYGLDKPLPVQFGYWL
                     REVLQGNLGTSIFLQRPVTQALWERAEPTTLLALLAVAIAALIGVPCGIVSAVFRARA
                     VDQFFTGIAMLGASIPSFWLGIVLIQIFAVSFGWFPVSGYGAPDAPFAERLHALVLPA
                     TVLGLLNSALIIRFTRASMLDVLGEDYVRTARSKGLSESVVVLKHALRNALVPIVTVI
                     GLTVALMIGGAVITETVFGLPGVGNLVVNAVLRRDYPVIQGALLVIAAIYVLINFSID
                     LLYAVVDPRVKV"
BASE COUNT          747 a         1405 c         1528 g          636 t
ORIGIN      
        1 gtgcgtgcgc cttgcacacc gatctcgagc atcacgtcaa aaggcacgct ggcaggattg
       61 cgcttcgccc agtcctcgac gagcgccagc tgcgccgtcg aatcaacgag gaacacgaca
      121 cgcaggtccg catgctgggc cagcagcatc tggatgcctg ccaggtcctc atcgctcacc
      181 acctggttgg cgatgagcgt gcgccgcgcg ccggccgcca cgcccacggc gagttgggtg
      241 acagtcgcaa aggtcagccc ccacgcaccg gcatcgagct ggcgctggaa cagctgcggc
      301 gacatggtcg tcttgccatg cggtgcgaga tcgatgcccc attcacgcac gcgcgattgc
      361 atccattgga ggttgtgttc gagcgcctcg cgcttgagca cggccagcgg aagcggcagg
      421 tcgcccgcca gcacgttcca gccggcagcg ccgacttcgc tgcgcaggcg cggcggctgg
      481 gtgcgcgggt agcccttgaa acggtcgctc agcagcgggt cgatgaagtc ttggatagca
      541 gaaacggtgt cggtcatggt cgtgcaacgg cggtgttgga gagcagttct ttgcggatgc
      601 aggcgctgta gtgtgctggg gaaatctcgt ccaggctggg caccgtctgc gcacacgcct
      661 cgatcgcatg cggacagcgc gtgcggaaca cgcagcccga aggcggcgag atcgggctcg
      721 gaatgtcgcc ctggagcgcg atgcgctcgg tggcaacttc ggggtcgggc ttcggactcg
      781 cggccagcag cgcccgggtg taggggtgcg agggattggc gaacagctcc tcggtggtgg
      841 ccacttccat caccttgccc aggtacagca ccaccacgcg gtcgcacagg tactccacca
      901 catccaggtc gtgcgagatg aacagcatcg tgagacccag gcgctcgcgc aggtccgcca
      961 gcaggttgat gacctgcgac tggatcgaca catccagcgc cgacagcggc tcgtcggcca
     1021 cgatgaactc cggctccacc gccagcgcgc gcgccacgcc gatgcgctgg cgctggccgc
     1081 ccgagaactc gtgcggaaag cggctcgcat gctcgggacg caggcctacg gtctccagca
     1141 actcggcgat gcgcttgtcg cgtgcggccg cgcccttgtg cagcccgtgc gtggacagcg
     1201 cctcgcccag gatcgcgctg atgcgcatct tcgggttcag gctggcgtac gggtcctgga
     1261 aaatgatctg cagcttgctg cgcagcttgc gcatgcgctc gcccgacagg ccgccgatgt
     1321 cgtcgccgcg atagagcacc tggccctcgg tgcgctcgac cagccgcagc acggtgcgcc
     1381 cgatggtgct cttgcccgag cccgattcgc ccaccaggcc cagcgtctcg ccggggtgga
     1441 tcgcgaagga cacgtcgtcc accgcgcgca cggggcgctc gctgctgccg aagtatttct
     1501 tcaggccgcg cacttcgatg aggggcgggg aagaaggaga agaggaaaca gaaacagcgg
     1561 tcatgcgacc ctcgcgagtt gggcgtcggg ctgcacgcgc acgcagcgcg cctggcgatc
     1621 ctggcggatg tcgatcagcg gcggcatggc gtccttgcag cttgccagcg cctggtcgca
     1681 gcgcggctcg aaggcacagc ccggcggcgg cgccaacgga ctggacacct gcccgcgaat
     1741 ggcgaacaag cgcttgggct tgggttgccc gggcacccgc gccttgcccg gcaggcaggc
     1801 cagcaggccc tgcgtgtacg ggtgttcggg ctgcgcgaac agaggccgca cgggcgcttg
     1861 ctcgaccaca cggcccgagt acagcaccac cacgtcgtcg gcatggtgcg ccaccacgcc
     1921 caggttgtgc gtgatgaaca ggatgctcat gcccgtctcg gcctgcaggc ggcgcatgag
     1981 ctgcaggatc tgcgcctgga tggtcacgtc cagggctgtg gtgggttcgt cggcgatcag
     2041 cagcgtcggg tcgcaggcca tcgccagcgc gatcatcacg cgctggcgca tgccgcccga
     2101 cagctggtgc gggtactcgt ggatgcgctg ggccgcggcg ggaatctcca ccagctccag
     2161 catgcgcagc gcatgggcca atgccgcctt gcggtccagc cccttgtgca gacgcacgct
     2221 ctcggcgatc tgctcgccga tggtgaagac cgggttcagg ctcgtcatgg gctcctggaa
     2281 gatcatcgac agctggttgc cgcgcagcga gcgcatctcg ggcccgccga tctgcaggag
     2341 gtccagcgtc ttgccttcgc gggtcacgaa gcaagccgat ccgctgacct gcgcgttcgc
     2401 ggtcttgggc agcaggcgca tcagcgtgag gctggtgacc gacttgcccg agcccgactc
     2461 gcccaccagc gcggtggtct tgccgggttg gatggcaaaa ctcacatcgg ccaccgagcg
     2521 gatcaggccg tcttcggtgg ggaagctggt gctcaggttg gtgaccgtga ggcgtgcgct
     2581 ttgagaggtg gtgttcgcgt tcgtcatcac gaggtcttct tgagcttcgg atcgagcagg
     2641 tcgcgcacgc catcgcccag cagctgcagc gacagcgccg tgaagatgat cgccagcccc
     2701 gggaacagca cgacccagaa ggcctggtgc gcatactgct ggctgcccgc gaccatggtg
     2761 ccccaggtgg ggatctcggg cggcacgccg acgcccagga acgacaggcc agcctcggcc
     2821 aggatggcgt acgcaaagat gaacgacacc tgcacgagga tcggcgacat caggttgggc
     2881 aggatgtggc gccacaggat gcgcgaggtg cgcacgccca gggcgcgcac ggcttcgacg
     2941 aagagaagtt cgcgcaccac cagggtcgag gcccgcacca cacgtgcgac acgcggggtg
     3001 tagaccagca ccagcgcgag cacggtgttc atcagcgagg ggccgaggat cgccaccagc
     3061 gcgatggcca gcaggatgtc gggaaaggac atcatggcgt cgaccacgcg catcaggggg
     3121 gcgtcgaggc ggcggaagaa cccggccatg aggcccagca ccgtgccggc gatgacggat
     3181 cccaaggcgg tgagcgcggc gatggccagc gagtagcgcg cgccgtgcac gatgcgcgcg
     3241 tacatgtcgc gccccagttc atcggtgccc aagaggtgct cggcgctggg cgccttcagg
     3301 cgctggagca cggcggtgtc gttggggtcg gcggaggcga acagcggcgc accgacggcg
     3361 agcaccgcaa tcaccagcag caccagggcg gacaccatca cgatgcggcg gtacatcagc
     3421 tggcggagca ttcgaggcat gcgcatcgtt cagaccttca cgcgcggatc gaccaccgca
     3481 tacagcaggt cgatcgagaa attgatgagc acatagatcg cggcgatcac gagcagcgcg
     3541 ccttgaatga cggggtagtc gcgccgcagc acggcgttga ccacgaggtt gcccacgccg
     3601 ggcaggccga agacggtctc ggtgatgacg gcgccgccga tcatgagcgc gaccgtcagg
     3661 ccgatcacgg tgacgatggg caccagggcg ttgcgcagcg cgtgcttcag aacgacgacg
     3721 ctctcgctca gccccttgga gcgcgcggtg cgcacgtagt cttcaccgag cacgtcgagc
     3781 atcgaggcgc gcgtgaagcg gatgatgagt gcggagttca gcaggcccag cacggtggcc
     3841 ggcagcacca gtgcgtgcaa tctctcggca aacggcgcat cgggcgcccc gtagcccgac
     3901 accgggaacc aaccgaagga caccgcgaag atctggatca gcacgatgcc cagccagaag
     3961 ctggggatgc tggcgccgag catcgcgatg ccggtgaaga actggtcgac cgcgcgggcg
     4021 cggaacacgg ccgagacgat gccgcagggc acgccgatga gcgccgcgat ggccaccgcc
     4081 agcagggcca gcaaggtggt gggctcggcg cgctcccaca gcgcctgcgt gaccgggcgc
     4141 tgcaggaaga tcgaggtgcc caggttgccc tgcagcactt cgcgcagcca gtagccgaac
     4201 tgcacgggca gtggcttgtc caggccgtac tccttctgca cgcgtgcgat gtcggcggcg
     4261 gtggcctggt cgcccagcag caccgagatc gggtcgccgg aggctgcgcg tgtgag
//
LOCUS       DATMSX010000544         9932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_112644,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000544 DATMSX010000000
VERSION     DATMSX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112644"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..977
                     /gene="tsf"
                     /locus_tag="VN041_17255"
     CDS             150..977
                     /gene="tsf"
                     /locus_tag="VN041_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWU30831.1"
                     /translation="MANFTIADIKALREQLGTGMVDTKKALEEADGDVEKATEILRLK
                     GAKGNAKRADRSTSEGLIAARESAGAVTLIELACETDFVAKNERFIALADKVADAVAA
                     VGADSVEAALAAPAGAQTVEQLISDEAAIIGEKVELRKVRTVKGENLSVYMHRTSKDL
                     PPQIGVAVAYSGTDAETARSIAQHISFANPSYLTREDVPAADVEKEREIVTEISRNEG
                     KPEAALPKIVEGRVTAYFKQVALLEQDYAKDNKLSVSQVAKDAGITVTDFARFKVGA"
     gene            1069..1785
                     /gene="pyrH"
                     /locus_tag="VN041_17260"
     CDS             1069..1785
                     /gene="pyrH"
                     /locus_tag="VN041_17260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWU30832.1"
                     /translation="MTERTGRRRVLLKLSGEAFGGGSLGVNPDVVSAIARDIAAAVDR
                     VEIAVVVGGGNFFRGAELSQRGMDRGRADYMGMLGTVMNALALQDFLEQAGAPTRVQS
                     AIQMTQVAEPYIPLRAERHMEKGRVVIFGAGAGLPYFSTDTVAAQRALEIGAQEVLVA
                     KNGVDAIYTADPNKDPDAERIERVTYRDALQRGLKVVDSTAFSLCMDNAMDMRVFGME
                     PAGNVTRALLGEPIGTLVTA"
     gene            1858..2412
                     /gene="frr"
                     /locus_tag="VN041_17265"
     CDS             1858..2412
                     /gene="frr"
                     /locus_tag="VN041_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWU30833.1"
                     /translation="MIADVLAETTGRMTRAVDAAKEDFATVRTGRANPQMFQKVLVDY
                     YGTPTALAQLASLANPEARSLIITPYDKSALKAIEQAIRDMPNLGANPSNDGNIVRVT
                     MPELTQERRKEYVKLVRSKGEDAKVHVRGIRRKAKDELDALKAEVGEDEIARGEKELD
                     ALTRQYVDAIDEALKRKEAELLEV"
     gene            2418..3671
                     /locus_tag="VN041_17270"
     CDS             2418..3671
                     /locus_tag="VN041_17270"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWU30834.1"
                     /translation="MSGENHSEEPRTGSPGSQNPDAASSAGRPAASGNEDAATPARGT
                     RRVDGDVPVTARPLSDAAFPEFDAARVPPRPPLPAAEPDPHTGPLSTADHSAIREQWR
                     ARRDEIESHVSQARDQLETHVSQARDHFDQANERIKQRTGRDLILAIVIGVAFGAALV
                     ASLLFIKWLFVPIALAAGLLGTYELSRALRAGGRRVDVVPQLIAATGVLLAGASGEFW
                     ISWVVLIVAVSFIAVWRMIAQMVVKDGRTYGDVLSDVMVGAFVQVYVPFLTSIAIMLL
                     FRENGQWWVLSFVAIAVAADTCAYAAGLAFGKHPMAPRISPKKTWEGFGGAVLGSLVA
                     GVLLATYLLHLQWWCGLVLGAVILLSATLGDLGESMLKRDLGIKDMSSWLPGHGGLLD
                     RLDSILPSTIPALALSYLLAPWMVS"
     gene            3671..4258
                     /locus_tag="VN041_17275"
     CDS             3671..4258
                     /locus_tag="VN041_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWU30835.1"
                     /translation="MSDSDLDVFDGDDEAAPPAFPLTSGRTRGYHRAAVDTFLDAARG
                     AFEDDRNDFGAEDVRDASFPLVRHGYDIAAVDAALGRVEDAFAARTRERAVRSLGVTE
                     WVARAREDAQRILDHLARPAHHRFARTGRLTFGYRVDEVDHVADRISGFLRDGDPLTV
                     EQVRGAAFRMQRGGYREEQVDALLDATVEVMLAVR"
     gene            4323..4991
                     /locus_tag="VN041_17280"
     CDS             4323..4991
                     /locus_tag="VN041_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWU30836.1"
                     /translation="MTPDNESISNPTRPNASGSTQKTPWRRRLSAVGAGLAVVGVSAA
                     LLAPTGTAIADPSTAADKAVTTFSLASEDTQNITVTVEGATIQAVDRGAFEVYVKPKP
                     TPKPTVASAPAKSGGGGGGGAARPKYTGGGTKEEWMAAAGIAPSDYPYVDYIVSRESG
                     WNPNATNKSSGACGLVQALPCSKVPGNGYNPVDNLRWGTGYATGRYGSWAGAYAFWTR
                     NHWW"
     gene            5001..5288
                     /locus_tag="VN041_17285"
     CDS             5001..5288
                     /locus_tag="VN041_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30837.1"
                     /translation="MARSHRRRPDRSRADESFDRLLAGWKRTESRRGQEWTVQPVSGA
                     SAVKEYICPGCGRSIAPGTAHLVAWRADGVMGDSADLAARRHWHTHCWRIG"
     gene            5285..6001
                     /locus_tag="VN041_17290"
     CDS             5285..6001
                     /locus_tag="VN041_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWU30838.1"
                     /translation="MTFEIRGPVLLPAERTDVELSTADGLTLVGELSMPSDREPTATL
                     VTLHPLPTAGGCMDTHILRKAAARLPAQAGMAVLRFNTRGTSSPRGRSEGAFDGGEAE
                     QFDVAAAMDFVRERGLPRPWLVGWSFGTELALKYGRDHEVEGAILLSPPLHRATDADL
                     SAWRGDERELVVLVPELDDYLRPDEARARFGLVPHARLIAVDGGRHLWVGESQTRRVL
                     TEIVAAVNPDALPLATVWDA"
     gene            complement(6005..7084)
                     /locus_tag="VN041_17295"
     CDS             complement(6005..7084)
                     /locus_tag="VN041_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWU30839.1"
                     /translation="MKIHSPFRTALVATLGVGLGIMLLTSVQMLSTVLLYIGTALFLS
                     LGLDPLVSFLERRGMPRWASVLTTIVAVLGVFTAIVLIILPVVIEQISQLVTKITDLI
                     VNGDQTIQNLKDWMTDTFPNLRVNDVFAYVEDWFSKEDWAKTVTEWGGSIGQGVLIVG
                     GAVLTGLFGAFIVLVLTIYLTASTPSLKRAVYQLVPASRRERFIDIADQITDSVGHYV
                     MGQVTLGVINGILSSIYLSIINAPFTAVLAVVAFFFSLIPLVGTLTGSTLIVLACLIP
                     GVGSPATALAAAIYYLIYMQIEAYFIAPRIMSRAVAVPGAVVVIAALSGGALLGLLGA
                     LIAIPVAASILIIYRQVLIPRMNER"
     gene            7246..9045
                     /locus_tag="VN041_17300"
     CDS             7246..9045
                     /locus_tag="VN041_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWU30840.1"
                     /translation="MRFVWAVVAFVLATVLIGAGIAQRTIFMGPTEQRMELTVDQPTP
                     YVLVDADVLRAHTGLQTLLVRGKGDVFVAYARTDDMKSWLSDTAYDHVSLTKSGKPKT
                     ARVEAEQPPADGGETSGRSPVGSDLWLDSFADKDSLVANLQLTKGNSVLIARDGVKPA
                     PTDILVSWALDTRTPWAGPLMAAGGLLLAVGIVLYILGIRHQRRGRGPRRKGPGPLPE
                     TQPITMPERAEIDQPGDAGTAQRTMTTARRRLAIALPALALTGLLATGCTADSWPQFG
                     GDAPSPTPSPSVVAPENQKQPVVTDKQGQRIVADVASTLAKADTDLDDKLAETRLTGA
                     ALDARRLEYQLRGKIEGREGALMMPLGKVKILLPEATEGWPRTVLALTVDEKDAKKPP
                     VLLTMVQSDPWANYRVTSMADMLAKSTFPNVAPAWLGTTRVPAESAFLSLPPSELAGA
                     FSDFVDADDKSQYATDFDEASKEFAKSVRDSRAAFVKKAADAGAGQTSAVAFKMAASD
                     DEPLSLATLDSGAVVSVSVTDTQTITPTNPDAVIKITDEPSKALTGVSEAAKGYTTTY
                     SIQLFFAVPAQGSNEQIRLLAYKQDLLSVQVIK"
     gene            9042..>9932
                     /locus_tag="VN041_17305"
     CDS             9042..>9932
                     /locus_tag="VN041_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWU30841.1"
                     /translation="MTEISAAALRGAVDLSSLRGRAAQPATAPDGAASAASGGVVVDV
                     TDASFGEILELSRSVPVVVDLWAEWCGPCKQLSPIIEKVTREQGGRVVLGKVDVDANP
                     QIAQAFRAQSIPLVVALIGGQPVPMFTGAVPEEQVREVFAKLLEVAAQNGVTGSVPTD
                     GEEPAAEPEEAPLPPLHAEAFAAIEAGDYARAIQAYQHALAENPRDEDAKAGLGQVRL
                     LDRVQGLDLQQVREAAAAAPLDVEAQFRVADLDLAGGHVDDAFSRLLDLFSAVDADQR
                     TPVRERLVELFDLIGAADPRV"
BASE COUNT         1616 a         3424 c         3422 g         1470 t
ORIGIN      
        1 ggctccggcc gctgacgagg cgccggccgc tgaggtcgtc gaggctccgg ccgctgacga
       61 ggctccggcc gccgaggccg ctgccgacgc agacgccaag taagcaacca gcacaccacg
      121 cacagacacg aagcaaggag ccaccacaca tggccaactt caccatcgcc gacatcaagg
      181 cgctgcgcga gcagctcgga acgggaatgg tcgacaccaa gaaggcgctc gaggaggctg
      241 acggcgacgt cgagaaggcc accgagatcc tgcgtctgaa gggtgccaag ggcaacgcca
      301 agcgcgctga ccgctcgacc agcgagggcc tgatcgccgc tcgtgagtcc gccggtgccg
      361 tgacgctgat cgagctcgcc tgcgagaccg acttcgtcgc caagaacgag cgcttcatcg
      421 ctctggccga caaggtcgcc gacgccgtcg cggctgtcgg cgccgactcg gtcgaggccg
      481 ctctggccgc ccccgcgggc gctcagaccg tcgagcagct catctccgac gaggccgcga
      541 tcatcggcga gaaggtcgag ctgcgcaagg tccgcaccgt caagggcgag aacctgtcgg
      601 tgtacatgca ccgcaccagc aaggacctgc ccccgcagat cggcgtcgcc gtcgcgtact
      661 ccggtaccga tgcggagacc gctcgctcga tcgctcagca catctcgttc gcgaacccct
      721 cctacctgac gcgcgaggat gtccccgcag ccgacgtgga gaaggagcgc gagatcgtca
      781 ccgagatctc ccgcaacgag ggcaagcccg aggccgccct gccgaagatc gtcgagggcc
      841 gcgtgaccgc gtacttcaag caggtcgccc tgctcgagca ggactacgcg aaggacaaca
      901 agctgtccgt gagccaggtc gcgaaggatg ccggtatcac cgtcaccgac ttcgcccggt
      961 tcaaggtcgg cgcgtaacac ctcggaaggg gttcggatcc acacgatccg aaccccttct
     1021 tcatgcccag aaggcactat cttgaatccg gacgagagga cccaccccat gactgaacgc
     1081 accggacgcc gtcgcgtcct tctcaagctc tccggcgagg ccttcggcgg cggatcgctg
     1141 ggtgtgaacc ccgacgtcgt cagcgccatc gcgcgcgaca tcgcagcggc ggtcgaccgg
     1201 gtcgagatcg ccgtcgtcgt cggcggcggc aacttcttcc gcggtgcgga gctgagccag
     1261 cgcggcatgg accgcggtcg cgccgactac atgggcatgc tgggcaccgt catgaacgcc
     1321 ctcgccctgc aggacttcct cgagcaggcc ggcgccccca cgcgtgtgca gtccgcgatc
     1381 cagatgactc aggtcgccga gccctacatc ccgctgcgcg cggagcgcca catggagaag
     1441 ggtcgcgtcg tgatcttcgg ggcgggcgcg ggcctcccgt acttctccac cgacacggtc
     1501 gccgcgcagc gagctctcga gatcggcgcg caggaggtcc tggtcgcgaa gaacggcgtg
     1561 gatgccatct ataccgccga cccgaacaag gaccccgacg cggagcggat cgaacgcgtg
     1621 acctaccgtg acgccctgca gcgcggcctg aaggtcgtcg actccaccgc gttcagcctg
     1681 tgcatggaca acgctatgga catgcgcgtg ttcggcatgg agccggccgg caacgtcacc
     1741 cgcgccctgc tgggcgagcc gatcggcacg ctcgtcacgg cctgaggcct ttcctccacg
     1801 gtccgccgag gcgtgccgga tagaatcttc tgaaaccccc gaacctaagg agcccccgtg
     1861 atcgcggatg tcctcgctga aaccaccgga cgcatgaccc gcgcggtcga tgccgccaag
     1921 gaggacttcg ccacggtccg caccgggcgt gccaacccgc agatgttcca gaaggtgctc
     1981 gtcgactact acggcacgcc gacggcgctg gcgcagctcg cgtcgctcgc gaaccccgag
     2041 gctcgctcgc tgatcatcac tccgtacgac aagtccgcgc tcaaggccat cgagcaggcg
     2101 atccgcgaca tgccgaacct gggcgcgaac cccagcaacg acggcaacat cgtccgcgtc
     2161 accatgcctg agctcacaca ggagcgccgc aaggagtacg tgaagctggt gcgctcgaag
     2221 ggcgaggacg cgaaggtgca cgtgcgcggc atccgccgca aggcgaagga cgagctggat
     2281 gcgctcaagg ccgaggtcgg cgaggacgag atcgctcgcg gcgagaagga gctggacgct
     2341 ctgactcgcc agtacgtcga cgccatcgac gaggccctca agcgcaaaga ggcagagctc
     2401 ctcgaggtct gatcgtcatg tccggcgaga atcactccga ggaaccgcgg accgggagtc
     2461 ccgggtcgca gaacccggat gcggcatcgt ccgcagggcg gcctgcggca tccgggaacg
     2521 aggatgccgc aacccccgcg cgcggcacgc gccgggtgga cggcgatgtg ccggtgacgg
     2581 cccgtccgct gtcggatgca gccttcccgg agttcgacgc ggcccgcgtg ccgccgcgtc
     2641 ccccgctgcc ggcggcggag cccgatccgc acacgggtcc gctgtcgacc gctgatcaca
     2701 gcgcgatccg cgagcagtgg cgggccaggc gcgatgagat cgagtcgcac gtgtcgcagg
     2761 cgcgagacca gttggagacg cacgtctccc aggcgcgtga ccacttcgac caggcgaacg
     2821 agcgcatcaa gcagcgcacc gggcgcgatc tgatcctcgc gatcgtgatc ggcgtggcct
     2881 tcggcgccgc actggtggca tcgctgctgt tcatcaagtg gctcttcgtg ccgatcgcgc
     2941 tggccgccgg cctgctgggc acctatgagc tctcccgcgc gctgcgcgcc ggcggccggc
     3001 gggtcgacgt cgttccgcag ctgattgcgg cgacgggcgt gctgctggcc ggagcatccg
     3061 gcgagttctg gatcagctgg gtggtgctca tcgtcgccgt gtcgttcatc gccgtgtggc
     3121 ggatgatcgc gcagatggtc gtcaaggacg gccgcaccta cggtgacgtg ctctccgatg
     3181 tcatggtcgg cgcgttcgtg caggtctatg tgccgttcct cacctcgatc gcgatcatgc
     3241 tgctgttccg tgagaacggt cagtggtggg tgctctcctt cgtggccatc gccgttgctg
     3301 ccgacacctg cgcctacgcg gccggtctcg ccttcgggaa gcatccgatg gcgccgagga
     3361 tcagccccaa gaagacctgg gagggcttcg gcggggcggt gctcggatcc ctggtcgccg
     3421 gtgtgctgct ggcgacctat cttctgcacc tgcagtggtg gtgcggtctc gtcctgggcg
     3481 ccgtgatcct gctctccgcc acgctcggcg atctcgggga gtcgatgctc aagcgcgatc
     3541 tcggcatcaa ggacatgagc tcctggctgc ccggtcacgg cggtctgctc gatcgtctgg
     3601 acagcatcct gccgtcgacg atccccgccc tcgccctctc atacctgctc gctccgtgga
     3661 tggtctcgtg atgtctgatt ctgatctcga tgtgttcgac ggcgatgacg aggccgcccc
     3721 tccggcgttc ccgctcacgt cggggcgcac ccgcggctat caccgcgcag ccgtggacac
     3781 gttcctcgac gccgcccgcg gcgcgttcga ggacgaccgg aacgacttcg gagccgagga
     3841 cgtgcgcgat gcctccttcc cgctggtccg gcacggctac gacatcgccg cggtggatgc
     3901 cgcgctggga cgcgtcgagg atgcgttcgc cgcacgcacc agggagcgcg ccgtgcgctc
     3961 gctgggggtc accgagtggg tcgcgagggc ccgcgaggac gcgcagcgca tcctcgacca
     4021 tctcgcgcgt cctgcccacc accggttcgc ccgcacgggg cgcctcacct tcgggtaccg
     4081 cgtcgacgag gtcgatcatg tcgccgaccg catcagcggt ttcctgcgcg acggcgaccc
     4141 cctcacggtg gagcaggtgc gcggcgcggc gttccggatg cagcgcgggg gataccgcga
     4201 ggagcaggtg gacgcactgc tcgacgccac cgtggaagtg atgcttgcgg tccgctgatc
     4261 tgccgccacg cggcggactc tcatggacgt cccagcgagt tctgcgtaga ctgaccacca
     4321 tcgtgactcc cgataacgaa tcgatatcga acccgacgcg cccgaacgcg tccggttcga
     4381 cgcagaagac tccctggcga cggcggctca gtgccgtcgg cgcgggtctc gcggtcgtcg
     4441 gcgtctccgc cgcactgctg gcgcccacag ggacggccat cgccgatccg agcaccgcgg
     4501 ccgacaaggc ggtgaccacg ttctccctcg cctccgagga cacgcagaac atcaccgtca
     4561 cggtcgaggg cgcgaccatc caggccgtcg atcgcggcgc gttcgaggtc tacgtcaagc
     4621 cgaagccgac ccccaagccc acggtcgcct cggcgcccgc gaagagcggc ggcggcggcg
     4681 gtggcggtgc agcacgcccg aagtacaccg gcggcggcac caaggaggag tggatggccg
     4741 cggcggggat cgccccgagc gactacccgt acgtcgacta catcgtctcg cgggagagcg
     4801 gctggaaccc gaacgcgacc aacaagtcct ctggggcctg cggtctcgtg caggcgctgc
     4861 cctgcagcaa ggtccccgga aacggctaca acccggtgga caacctgcgc tggggcaccg
     4921 gctacgcgac gggtcgttac ggcagctggg cgggcgcgta cgcgttctgg acccgcaacc
     4981 actggtggta gaccggagga atggcacgat cccaccggcg gcgtcctgac cgctcccgcg
     5041 cagacgagtc cttcgaccgc ctcctggcgg gctggaagcg caccgagagt cgtcgtggcc
     5101 aggaatggac ggtgcagccc gtgtcgggtg catcggccgt caaggagtac atctgcccgg
     5161 gctgcggtcg cagcatcgcg ccgggcacgg cgcatctggt cgcctggcgc gccgacggcg
     5221 tgatgggcga ctcggccgat ctcgctgcac gtcgccactg gcacacgcac tgctggagga
     5281 tcggatgacc ttcgagatcc gcgggcccgt cctgctgcct gctgagcgca ctgacgtcga
     5341 gctgtccacc gcggatggac tgacactggt cggcgagctg tcgatgccgt cggacaggga
     5401 accgacggcg acgctggtga cgctgcatcc gctgcccacg gccggcggat gcatggacac
     5461 ccacatcctg cgcaaggcgg ccgcccggct gcccgcccag gcggggatgg ccgtgctgcg
     5521 cttcaacacc cgcggaacct cgtcgcctcg tggcagaagc gagggcgcat tcgacggggg
     5581 agaggccgag cagttcgatg tggccgcggc gatggacttc gtccgtgagc gcgggcttcc
     5641 ccgcccctgg ctggtcggct ggtcgttcgg cacggagctg gcactcaagt acggccgcga
     5701 ccacgaggtc gagggcgcga tcctcctctc cccgcccctg caccgtgcga ccgacgccga
     5761 cctgtccgcg tggcggggtg acgagcgcga gctggtcgtg ctcgtccccg agctggacga
     5821 ctacctgcgt cccgatgagg cccgcgcgcg gttcgggctg gtgccgcacg cccggctcat
     5881 cgctgtcgac ggcggacggc acctgtgggt cggcgagagc cagaccaggc gggtgctgac
     5941 cgagatcgtc gcagcagtga accccgatgc gctgccgctg gcgacggtct gggacgcctg
     6001 agagtcagcg ctcgttcatg cgcgggatca gcacctgccg gtagatgatc aggatgctgg
     6061 ccgcgacggg gatcgcgatg agcgcgccga gcagtcccag cagcgcacca ccggacagcg
     6121 ccgcgatgac gacgacggcg ccgggcacgg ccacggcgcg gctcatgatg cgcggggcga
     6181 tgaagtacgc ctcgatctgc atgtagatga ggtagtagat cgccgccgcc agcgccgtgg
     6241 ccggggagcc gaccccgggg atcaggcagg cgagcacgat cagagtcgag cccgtgagtg
     6301 tgcccaccag cgggatcagc gagaagaaga acgccaccac ggcgagcaca gccgtgaagg
     6361 gcgcattgat gatcgagagg tagatcgagc tcaggatgcc gttgatcacg ccgagcgtga
     6421 cctgacccat cacgtagtgc ccgacggagt cggtgatctg gtcggcgatg tcgatgaacc
     6481 gctcccggcg cgacgccggg acgagctggt acaccgcgcg cttgagcgac ggggtggacg
     6541 cggtcaggta gatcgtgagc acgagcacga tgaacgcgcc gaagagaccg gtcagcacgg
     6601 cgccgccgac gatcagcacg ccctgtccga tcgagccgcc ccattcggtg acggtcttgg
     6661 cccagtcctc tttggagaac cagtcctcca cgtaggcgaa gacgtcgttc acacgcaggt
     6721 tcgggaacgt gtccgtcatc cagtccttga ggttctggat cgtctggtcg ccgttcacga
     6781 tcaggtcggt gatcttcgtg accagctggg agatctgctc gatgacgacg ggcaggatga
     6841 tcagcacgat cgcggtgaac accccgagca cggcgacgat cgtggtgagg acggatgccc
     6901 agcggggcat gcccctgcgc tccaggaacg acaccaacgg gtccaggccc aggctgagga
     6961 acaaggccgt gccgatgtac agcagcaccg tcgagagcat ctgcacgctg gtgagcagca
     7021 tgattcccag gcccacgccg agcgtggcca ccagagcggt gcggaaaggg ctgtggatct
     7081 tcatcgttct cccggtcgga tgcgagcaag cctaatcgcc tgcgatcctc aagccgtcca
     7141 cgacgggccg atgcgctcgt gggcaatgct caggtgcttt cgatagtctg gaacgtcgag
     7201 cggggacccc tggaacgcgt ccggggtcac gtaaggagtt gattcgtgcg attcgtatgg
     7261 gccgtcgtgg ccttcgtgct ggccacggtt ctcatcggag cgggaatcgc tcagcggacc
     7321 atcttcatgg gaccgaccga gcagcggatg gagctgacag tcgatcagcc cactccgtat
     7381 gtgctcgtcg atgccgacgt gctgcgtgcc cacaccggtc tgcagacgct gctggtgcgc
     7441 ggcaagggcg atgtcttcgt ggcctacgcc cgcaccgacg acatgaagtc ctggctgtcc
     7501 gacaccgcct acgaccacgt gtccctgacc aagtcgggga agccgaagac ggcgcgcgtc
     7561 gaggcggagc agccgcccgc cgacggcggt gagacgtccg gccgctcgcc ggtcggatcg
     7621 gacctctggc tcgattcgtt cgccgacaag gactcgctgg tcgcgaatct gcaattgacc
     7681 aagggcaaca gcgtgctgat cgcccgcgac ggcgtcaagc cggcgccgac cgacatcctc
     7741 gtctcctggg cgctggacac gcgcacaccc tgggcgggtc cgctgatggc cgccggcgga
     7801 ctgctgctgg ccgtcggcat cgtgctgtac atcctcggca tccgtcacca gcgccgcggg
     7861 cgcggcccgc gccgcaaggg tccgggaccg ctgcccgaga cgcagccgat cacgatgccg
     7921 gagcgcgccg agatcgatca gccgggagac gcgggcactg ctcagcggac catgacgacg
     7981 gcgcgtcgcc gtctggcgat cgctctgccg gctctcgcgc tgaccgggct gctcgccacc
     8041 gggtgcacgg ccgactcctg gccccagttc ggcggtgacg ccccctcgcc gacgccgtcg
     8101 cccagtgtgg tcgcgccgga gaaccagaag cagcccgtcg tcaccgacaa gcagggccag
     8161 cggatcgtcg ccgacgtcgc ctcgacgctg gccaaggccg acaccgacct ggacgacaag
     8221 ctcgcggaga cgcgcctcac cggagccgcg ctcgacgccc gccggctgga gtaccagctg
     8281 cgcgggaaga tcgagggtcg cgaaggcgcc ctcatgatgc cgctgggcaa ggtgaagatc
     8341 ctgctccccg aggcgaccga ggggtggccg cgcaccgtgc tcgcgctgac cgtggacgag
     8401 aaggatgcca agaagccacc ggtgctcctg accatggtgc agagcgaccc gtgggccaac
     8461 taccgggtca cctcgatggc tgacatgctg gccaagagca ccttcccgaa tgtggcgccc
     8521 gcctggctgg gcaccacccg cgtcccggcc gagtcggcgt tcctgtcgct gcccccgtcg
     8581 gagctcgccg gtgcgttctc cgatttcgtg gacgccgatg acaagagcca gtacgccacc
     8641 gacttcgacg aggcgtccaa ggagttcgcg aagtcggtcc gggacagtcg cgccgcgttc
     8701 gtcaagaagg cggcggatgc cggggcgggc cagacctctg ccgtcgcgtt caagatggcg
     8761 gcatccgacg acgagccgtt gtcgctggcg accctcgaca gcggcgcggt ggtcagcgtg
     8821 agcgtcaccg acacgcagac gatcaccccg acgaacccgg acgcggtgat caagatcacc
     8881 gatgagccct cgaaggcgct gaccggcgtc tccgaagccg cgaagggcta cacgacgacg
     8941 tacagcatcc agctgttctt cgccgtgccc gcgcagggct cgaacgagca gatccgtctg
     9001 ctcgcctaca agcaggacct tctcagtgtg caggtgatca agtgaccgaa atctccgctg
     9061 ccgcacttcg cggcgccgtc gacctctcct cgctgcgcgg ccgcgcagcc cagcccgcga
     9121 ccgctccgga cggggccgcg tcggcggcgt ccggtggggt cgtcgtcgat gtgaccgacg
     9181 cgagcttcgg cgagatcctc gagctctcgc ggtccgtgcc cgttgtcgtc gatctgtggg
     9241 ccgaatggtg cggcccgtgc aagcagctca gccccatcat cgagaaggtg accagggagc
     9301 agggcggccg ggtggtgctc ggcaaggtcg acgtcgacgc gaacccgcag atcgcgcagg
     9361 cgttccgcgc gcagtcgatc ccgctggtcg tggccctcat cggcggtcag ccggtgccga
     9421 tgttcacggg cgcggtgccc gaggagcagg tgcgcgaggt gttcgcgaag ctgctcgagg
     9481 tcgccgccca gaacggcgtg accggaagcg tccccacgga cggcgaggag ccggctgcgg
     9541 agcccgagga ggcgccgctg ccgccgttgc atgccgaggc gttcgccgcg atcgaggcgg
     9601 gcgactacgc acgcgccatc caggcgtatc agcacgcgct ggctgagaac ccccgcgacg
     9661 aggatgccaa ggccgggctc ggccaggtgc gtctgctgga ccgtgtgcag ggtctcgacc
     9721 tgcagcaggt tcgcgaggcc gccgcggccg ctccgctcga cgtcgaggcg cagttccgtg
     9781 tcgccgacct ggatctcgcc gggggacacg tggacgacgc gttctcccgc ctgctggacc
     9841 tgttcagcgc ggtggacgcc gaccagcgga cgcccgtgcg tgagcgcctc gtcgagctgt
     9901 tcgatctgat cggcgccgcg gatccgcgcg tc
//
LOCUS       DATMSX010000545         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_94115,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000545 DATMSX010000000
VERSION     DATMSX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94115"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VN041_17310"
     CDS             <1..139
                     /locus_tag="VN041_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HWU30842.1"
                     /translation="SQDARSVLRFSLGRTSTDADVDAVLAVIADAYARASGRRAGLAA
                     G"
     gene            254..1351
                     /gene="mnmA"
                     /locus_tag="VN041_17315"
     CDS             254..1351
                     /gene="mnmA"
                     /locus_tag="VN041_17315"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWU30843.1"
                     /translation="MRVLAAMSGGVDSAVAAARAVEAGHDVVGVHLALSRAGGTLRTG
                     SRGCCTIEDALDARRVADLLGIPFYVWDFSERFRDDVIEDFISEYRAGRTPNPCLRCN
                     EKIKFAALLERALELGFDAVCTGHYATLVDGAGGLELHRASDSAKDQSYVLGVLNAQQ
                     LAHTYFPLGSTPSKAIVRAEAAERGISVAQKPDSHDICFIPDGDTRGWLAEKVGAETG
                     EVVDRTGAVVGTHEGAHAFTVGQRRGLRLGVPAADGKPRFVLEVRPVTNTVVVGPKEA
                     LAIAEISGERFSWAGPAPRESEFACEVQIRAHADPVPATAFVTDEGVRVVPEHPLDGV
                     APGQSAVIYVGTRVLGQFTIDTTVSAVPVEA"
     gene            1512..2567
                     /locus_tag="VN041_17320"
     CDS             1512..2567
                     /locus_tag="VN041_17320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWU30844.1"
                     /translation="MMTPRRILEAVTTIALIAAGLAGAPGAASAATPPPETPIPYAGT
                     VAYRAVGLDLGTAKFTVLSVARLKNATVLYYALTDGTPGADSWLYTFEPHLGTPYRPG
                     SSYQIGLIDAAGGKMYMPLEDEGTCLCVLPASLEAPSGGTEPVVGYAVMPALPASVKR
                     VTVQIGGNAIIPDVPVSKAPPKGEHGKDPVLLGTWPALPSASAIEAADPGRVTLDLFE
                     NIADEQAATSHSSTQTAVALNSDVLFEFNRSDLTDAAREALEKVAADIDAHAAGPVSI
                     TGYTDDVGTDSYNLDLSQRRADAVLAALKPLVKNTSITFTTAGRGEQDPVAENGTDQG
                     RAQNRRVTVAYDTKEEQ"
     gene            2567..3157
                     /locus_tag="VN041_17325"
     CDS             2567..3157
                     /locus_tag="VN041_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30845.1"
                     /translation="MKRTHGALAGGLAVIGMLLAGCTTTPSPVPKPTTTQGAGLTAKQ
                     VAKKIKDTVLPANSLASTTGTISAPGISTQVRIDVEEVRALADSTMLVWRLSTASGEQ
                     EDVTSFQFAVKPFLDTRNVALMLDGGAKTLRPFTFRYENDSEAAMSCLCGKPAHASDG
                     TGLQLFALMPPLPKGARAVDVSMPGFDVMKDVPVSR"
     gene            3198..>4651
                     /locus_tag="VN041_17330"
     CDS             3198..>4651
                     /locus_tag="VN041_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWU30846.1"
                     /translation="MPENISLEDARSEAEELTTRILDAKDAYYGRDTSLVDDATYDAW
                     MRRLEELERLHPELQGQDSPTQMVGAAESTGLDTIEHAERMLSLDNVFSLDELRDWEA
                     KTRAAAGRDVAWLTELKIDGLAINLRYENGVLTSAATRGDGRVGEIVTENALRLPEIP
                     ERLSGTGHPEIVEVRGEVFIPVAAFERLNAAQAAFRDRAYADALTRWEARGGAGKKPF
                     DEEKARAAAARRFPSFANPRNAASGGLRQQLDKKNGLELEAGLLRTESLALYVHGIGA
                     WPNPPVAAQSEVYELLAEWGLPTSPHTKVCHSTDEVAEFVAHYGEHRHDIEHELDGIV
                     VKVDELALHAELGETSRAPRWAIAYKYPPEEVQTKLLDIVVSVGRTGRATPFAVMAPA
                     HVAGSVVRQATLHNKDVVKAKGVLIGDTVVLRKAGDVIPEVLGPVVERRDGTEREFVM
                     PVGCPECGTPLRPMKEGDIDLRCPNARSCPAQVRG"
BASE COUNT          745 a         1583 c         1667 g          656 t
ORIGIN      
        1 atctcaggac gcgcgcagcg tgctgcgctt ctcgctcggc cgcacctcca cggacgcaga
       61 cgtcgacgcc gtgctcgccg tgatcgcgga cgcgtacgcg cgggcatccg gccgtcgggc
      121 cgggctcgcg gccggctgaa ccactccgtg acgagcgcac cgcttgtcga cgtcggcagg
      181 tggttcggtc gtcggcaggt ggtgctctcg gtatccggcc ctcggtcagg tgcgcgttcg
      241 tagactggat gtcatgcgtg ttctggcggc gatgagcggt ggggtcgatt cggcggtggc
      301 agcggcccgc gccgtcgaag ccggccacga cgtcgtcggc gtgcacctgg cgctgtcccg
      361 tgccggcggg acgctgcgca ccggcagccg cggatgctgc acgatcgagg atgccctgga
      421 tgcgaggcgc gtggccgacc tgctcggcat cccgttctac gtgtgggact tctccgagcg
      481 cttccgcgat gacgtgatcg aggacttcat ctcggagtac cgcgccggtc gcaccccgaa
      541 cccgtgcctg cgctgcaacg agaagatcaa gttcgccgcc ctcctggagc gcgcgctgga
      601 gctcggcttc gacgcggtct gcacggggca ctacgcgacg ctggtcgatg gcgccggcgg
      661 cctggagctg caccgggcgt ccgacagcgc caaggaccag tcctacgtgc tcggcgtgct
      721 gaacgcgcag cagctcgcgc acacctactt cccgctgggc tccacgccgt ccaaggcgat
      781 cgtgcgcgct gaagcggccg agcgcggcat cagcgtggcg cagaagcccg acagccacga
      841 catctgcttc atccccgacg gcgacacgcg cggctggctg gctgagaagg tcggcgcgga
      901 gaccggcgag gtggtcgacc gcaccggagc cgtggtgggc acgcacgagg gcgcgcacgc
      961 gttcacggtg ggtcagcgac gagggctgcg cctgggcgtt cccgccgccg acgggaagcc
     1021 gcggttcgtg ctggaggtgc gtccggtcac gaacacggtc gtggtcggtc ccaaggaggc
     1081 gctcgcgatc gcggagatct cgggagagcg gttctcctgg gcgggccccg cgccccggga
     1141 atccgagttc gcctgcgagg tgcagatccg cgcccacgcc gacccggtgc ccgcgaccgc
     1201 gttcgtgacg gatgaggggg tgcgcgtcgt gcccgagcat ccgctggacg gcgtcgctcc
     1261 cggccagagc gccgtgatct atgtcggcac gcgcgtgctc ggacagttca cgatcgacac
     1321 caccgtgtcg gccgttcccg tggaggcctg agactgccga cagcacgagc cgccgcggcg
     1381 atggggagaa gaccccatcg acatcggcgg gctccgcgat gacgctggtg aacgcacgtg
     1441 tcgccggagc atctgcggcg gcgatggtgc gagaccgcgc ggcgagccgg agggctcagc
     1501 acggggggag catgatgaca ccacgacgga ttctcgaggc agttacgacg atcgcgctga
     1561 tcgcggcagg actggcgggg gcgccgggtg cggcatccgc tgccacaccg ccgccggaaa
     1621 ccccgatccc gtacgcgggc accgtcgcct accgtgcggt gggactcgac ctgggcaccg
     1681 cgaagttcac cgtgctctcg gtggcgcggc tgaagaacgc gaccgtgctc tactatgcgc
     1741 tgacggacgg gacgccggga gctgactcct ggctgtacac gttcgagccg cacctgggta
     1801 ctccataccg tccgggatcg tcttaccaga tcggtctgat cgacgccgcc ggcgggaaga
     1861 tgtacatgcc actggaggac gagggaacgt gcctgtgcgt cctgccggcg tctctcgagg
     1921 ccccgtccgg cggcaccgag ccggtcgtcg ggtacgcggt gatgccggcg ctgccggcat
     1981 ccgtgaagcg cgtcacagtg cagatcggcg ggaacgcgat catcccggat gtgccggtgt
     2041 cgaaggcgcc cccgaagggc gagcacggca aggaccccgt gctgctcggt acgtggcccg
     2101 cgctgccctc ggcatccgcc atcgaggctg cagacccggg cagagtcacg ctggacctgt
     2161 tcgagaacat cgccgacgag caggcggcga cctcgcacag cagcacgcag acggcggtcg
     2221 ctctgaactc cgatgtgctg ttcgagttca acaggtcaga tctcacggac gcagccagag
     2281 aggccctcga gaaggtcgcc gccgacatcg atgcgcacgc ggcaggtccc gtctcgatca
     2341 ccggctacac cgacgacgtc ggcaccgatt cgtacaacct cgacctgtcg cagcgcagag
     2401 cggatgccgt gctggcggcg ctcaagcctc tcgtgaagaa cacgtcgatc accttcacca
     2461 ccgccggacg cggcgagcag gacccggtgg ccgagaacgg cacggatcag ggtcgggcgc
     2521 agaaccgccg cgtgaccgtg gcgtacgaca cgaaggagga gcagtgatga agcgcacgca
     2581 cggcgctctc gcaggcggac tcgccgtgat cggcatgctg ctcgccgggt gcacgacgac
     2641 gccgtccccg gtgccgaagc cgacgacgac gcagggcgcc ggtctcacgg cgaagcaggt
     2701 cgcgaagaag atcaaggaca cggtgctgcc ggcgaactcg ctggccagca ccaccggcac
     2761 gatcagcgcg cccgggatct ccacgcaggt gcggatcgat gtcgaggaag tccgcgcgct
     2821 ggcggactcc accatgctcg tctggcggtt gtcgacggca tccggggagc aagaggacgt
     2881 cacctccttc cagttcgcgg tgaagccgtt cctcgacacc cgcaacgtgg cgctcatgct
     2941 ggacggtggc gcgaagaccc tgcgtccgtt caccttccgg tacgagaacg actcggaggc
     3001 ggcgatgtcg tgtctgtgcg gaaagccggc gcacgcctcc gacgggacgg gcctgcagct
     3061 gttcgcgctc atgccgccgc tgccgaaggg cgccagagct gtcgacgtct cgatgcccgg
     3121 tttcgatgtg atgaaggacg tgccggtgag tcgctgaggt ccccacccgt gtcggtgctc
     3181 gctcgtagac ttgcggggtg ccggagaaca tctcactgga agacgcccgc agcgaggccg
     3241 aggagctgac cactcgcatc ctcgacgcca aggacgcgta ttacggacgc gacacatcgc
     3301 tggtcgacga cgccacctac gacgcctgga tgcgcaggct ggaggagctc gaacggctgc
     3361 accccgagct gcagggccag gattcgccca ctcagatggt gggcgcggcc gagtccacgg
     3421 ggctggacac catcgagcat gccgagcgga tgctcagcct ggacaacgtg ttctcgctcg
     3481 acgagctgcg ggactgggag gccaagacgc gcgccgcggc cggacgcgac gtcgcgtggc
     3541 tcaccgagct gaagatcgac ggcctggcga tcaacctgcg ctacgagaac ggcgtcctca
     3601 cttcggccgc cacccgcggt gacggacgag tcggcgagat cgtcaccgag aatgcgctgc
     3661 gcctgcctga gatccccgaa cggctctccg gcaccgggca tccggagatc gtcgaggtgc
     3721 gcggcgaggt cttcatcccg gtcgccgcct tcgagcgtct caacgccgcg caagcggcct
     3781 tccgcgaccg cgcctacgcc gacgcgctga ctcgctggga agcgcgcggc ggcgcaggca
     3841 agaagccgtt cgacgaggag aaggccaggg cggccgccgc acgccgtttc ccgtccttcg
     3901 ccaacccgcg caacgccgcc agcggtggcc tccgtcagca gctcgacaag aagaacggcc
     3961 tcgagctgga ggcggggctg ctccgcaccg agtcgctcgc gctgtacgtg cacggcatcg
     4021 gcgcctggcc gaacccgccg gtcgcggcgc agagcgaggt gtacgagctg ctcgcggaat
     4081 gggggctgcc gaccagtccg cacacgaagg tgtgccactc gaccgacgag gtcgccgagt
     4141 tcgtcgcgca ctacggcgag caccggcacg acatcgagca cgaactggac ggaatcgtcg
     4201 tcaaggtcga cgagctggca ctgcacgcgg agctggggga gaccagccgg gctccgcgct
     4261 gggcgatcgc ctacaagtac ccgcctgagg aggtgcagac gaagctgctc gacatcgtcg
     4321 tctcggtcgg tcgcaccggg cgcgcgacgc cgttcgcggt gatggcgccc gcgcacgtcg
     4381 cggggtccgt ggtgcgccag gcgaccctgc acaacaagga cgtcgtcaag gccaagggcg
     4441 tgctgatcgg cgacaccgtg gtgctgcgca aggcgggaga cgtgatcccc gaggtgctcg
     4501 gccctgtggt cgagcgccgc gacggcaccg agcgcgagtt cgtcatgccc gtcggatgcc
     4561 cggagtgcgg cacgccgctg cgccccatga aggagggcga catcgacctg cgctgcccga
     4621 acgcgcgctc ctgcccagcg caggtgcgcg g
//
LOCUS       DATMSX010000546         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_56072,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000546 DATMSX010000000
VERSION     DATMSX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56072"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1193
                     /locus_tag="VN041_17335"
     CDS             342..1193
                     /locus_tag="VN041_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU30847.1"
                     /translation="MTSTQNATMSLALAQAPGEPNAFVRWWQRSWHPVVFVIAVLFIW
                     WLVTTLKWVKPFIIPSPGDTWAAFANNAAYLAQHTWVTTYETLVGFVIAVIVGLFVAV
                     IMVYSRGLEQTLYPVILFAQVIPKIAIAPLFIVWLGFGMEPKILVAVLMAFFPVVISG
                     LAGLRSVDPEILQLTSTMGAGRWRTFTKVRLPAALPELLSGLKVAATLAVTGAVVGEF
                     VGANEGLGYVILQANGNLDTAMLFAALIIMSLMGVLLFAIIQIAEKFLIPWHASKRDV
                     GAATVRL"
     gene            1241..2281
                     /locus_tag="VN041_17340"
     CDS             1241..2281
                     /locus_tag="VN041_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30848.1"
                     /translation="MKKSASILGTLAVITASALALVGCGASAPAGDGGAPAKGDKQLT
                     DVTLMLNWYPYGEHAPFYYGVEKGIFKDHGINLTIKAGQGSTKTAQAVGQKQVDFGWA
                     DTPAVLANIDKGVAIKSVGVFLQTTPSAVQVFADSGIEKPKDLEGKTIAVSAGDAPTT
                     TFPLYLEAVGLKESDVKQQNLDPAGKIAAVLSGQVDGLIGFAHDQGPTIAAKSGKKMT
                     YLRYSDAGLNFYSNGLIAPDDTIKDKPELVKEMVAATSEAFAAAQKDPEAAVASMAGK
                     DPQMPAKDVLLDQWNETIKLLSTKATEGKAPGTNATEDWQATLDVLSKAGLISGDGDV
                     KTYFDASFAPGK"
     gene            2320..3099
                     /locus_tag="VN041_17345"
     CDS             2320..3099
                     /locus_tag="VN041_17345"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30849.1"
                     /translation="MTQTSAALEITDVSITFASKRGRVSALEGVDLDVQAGEFITIAG
                     PSGCGKSTLLKAVAGLTLPSSGRVRLNGEDVTGPRQDIGYVFQRAALLEWRSVRGNIL
                     LQAEMRGMDSKKAQARADELIEMTGLTGFEKALPHELSGGMQQRVSLCRALLHEPRVL
                     LMDEPFGALDALTREKMNVELNRIWAQTGTTVLLVTHSVAEAVYLASRVVVMSPRPGR
                     ILEEHRVDLPAERGYAHTLETPEFTRISSRVRELLGSPGGD"
BASE COUNT          542 a         1153 c         1065 g          484 t
ORIGIN      
        1 cgcgactgac cggcagcccg gacgccggtc caggcccgcc tgcgtgcgct gcgcgcggcc
       61 gacaggtacg cggtgcggag aggcgacgaa cgggtacgga tgcacgaccg gggaacacct
      121 gcacgatcga ttcggcgatc cggtcgtgca atcgttcccg cgtcgtgcag ctgtgccggg
      181 tcagcgcgca cgtgcaggtc cgtgcccgcc tgtgcgcgcc tgcgcgggcc cgcacccggt
      241 cggattgaca ctccccgata cgcgacatag actcgcagca acggttcgga atacgctttc
      301 ccgaaatcga tctcatcatt gacgacgacg tcgaggaccc catgacttca acgcagaacg
      361 ccaccatgtc gctcgcgctc gcccaggcgc cgggtgaacc gaacgccttc gtccgctggt
      421 ggcagcgcag ctggcatccg gtcgtgttcg tgatcgccgt gctgttcatc tggtggctcg
      481 tcaccaccct gaagtgggtc aagccgttca tcatcccctc gcccggcgac acctgggcgg
      541 cgttcgcgaa caacgcggcg tacctcgcgc agcacacctg ggtcaccacc tacgagacgc
      601 tggtcggatt cgtcatcgcg gtgatcgtcg gcctgttcgt cgccgtgatc atggtgtact
      661 cccgcgggct cgagcagacc ctctaccccg tcatcctttt cgcgcaggtc atccccaaga
      721 tcgccatcgc gccgctgttc atcgtgtggc tcggattcgg catggagccc aagatcctgg
      781 tcgccgtgct gatggcgttc ttccccgtcg tgatctccgg cctcgcggga ctgcgctcgg
      841 tcgaccccga gatcctgcag ctgacgtcca cgatgggcgc cggtcgctgg cgcaccttca
      901 ccaaggtgcg actgcccgcg gcactgccgg agctgctctc cggactcaag gtggcggcca
      961 cgctcgccgt caccggcgcc gtggtcggcg aattcgtcgg tgcgaacgag ggcctcggct
     1021 acgtcatcct gcaggccaac ggcaacctcg acacggcgat gctgttcgcc gcgctcatca
     1081 tcatgtcgct gatgggcgtc ctgctgttcg cgatcatcca gatcgccgag aagttcctca
     1141 tcccgtggca cgcgtccaag cgcgacgtcg gcgccgcgac cgtccgcctc tgacccctgt
     1201 cagcacccgt accctgcacc atcacacaca aggagcactg atgaagaagt cagcatccat
     1261 cctcggcacc ctcgccgtca tcacggcatc cgctctcgcg ctggtcggct gcggcgcctc
     1321 cgcgccggca ggcgacggcg gtgcgcccgc gaagggcgac aagcagctca ccgacgtcac
     1381 gctcatgctc aactggtacc cgtacggcga gcacgccccg ttctactacg gcgtcgagaa
     1441 gggcatcttc aaggaccacg ggatcaacct gaccatcaag gccggtcagg gatccaccaa
     1501 gaccgcgcag gccgtcggcc agaagcaggt cgacttcggc tgggccgaca cgcccgccgt
     1561 gctcgccaac atcgacaagg gcgtggcgat caagagcgtc ggcgtcttcc tgcagaccac
     1621 gccgtcggct gtgcaggtct tcgccgactc cggcatcgag aagccgaagg acctcgaggg
     1681 caagacgatc gcggtctcgg cgggcgacgc cccgaccacg acgttcccgc tgtacctcga
     1741 ggcggtcggg ctgaaggagt ccgacgtcaa gcagcagaac ctcgacccgg ccggcaagat
     1801 cgcggccgtg ctctccggcc aggtcgacgg gctgatcggc ttcgcgcacg accagggccc
     1861 gacgatcgcg gcgaagagcg gcaagaagat gacctacctg cgctactcgg acgccgggct
     1921 gaacttctac agcaacggcc tgatcgcgcc cgacgacacc atcaaggaca agcccgagct
     1981 ggtcaaggag atggtggccg ccacctccga ggcgttcgcc gccgcgcaga aggaccccga
     2041 ggcggccgtg gcgtcgatgg caggcaagga cccgcagatg ccggcgaagg acgtgctgct
     2101 ggaccagtgg aacgagacca tcaagctgct cagcaccaag gcgaccgagg gcaaggcgcc
     2161 cggaaccaac gccaccgagg actggcaggc cacgctcgac gtgctgtcga aggccggcct
     2221 gatctcgggc gacggcgacg tgaagaccta cttcgacgcg tccttcgccc ccggcaagtg
     2281 accgcggcac gcccgtaccc cgatcaggag atgaccgaca tgacgcagac gagcgcggcg
     2341 ctggagatca ccgatgtctc gatcaccttc gcctccaagc gcggacgggt gtcggcgctg
     2401 gagggcgtcg acctggacgt gcaggcgggg gagttcatca ccatcgccgg gccctcgggc
     2461 tgcggcaagt ccaccctgct gaaggccgtc gccggcctga ccctgccgag ctccggcaga
     2521 gtcaggctca acggcgagga cgtgaccggc cccaggcagg acatcggcta cgtgttccag
     2581 cgcgcggctc tgctcgagtg gcgcagcgtg cgcgggaaca tcctgctgca ggccgagatg
     2641 cgcggcatgg actcgaagaa ggcgcaggcc cgcgccgacg agctgatcga gatgaccggc
     2701 ctgaccggct tcgagaaggc cctcccgcac gagctgtccg gcggcatgca gcagcgtgtg
     2761 tcgctgtgcc gtgccctgct gcacgagccg cgggtgctgc tgatggacga gccgttcggc
     2821 gcgctcgatg ccctgacgcg cgagaagatg aacgtcgagc tcaaccgcat ctgggcgcag
     2881 accggcacca ccgtgctgct ggtcacccac tcggtggccg aggcggtcta cctcgccagc
     2941 cgggtggtcg tgatgagccc gcgccccggg cgcatcctcg aggagcatcg cgtggacctg
     3001 cccgccgagc gcggctacgc gcacacgctc gagacgcccg agttcacccg gatctccagt
     3061 cgcgtgcgcg aactgctggg ctcgcccggc ggcgactgac gggcacggat gcacgacccg
     3121 ggaacacctg cacgatcgaa gccgtgatcc ggtcgtgcat ccgttcccgg gtcgtgcagg
     3181 tgctcccgcg cgtgcggcgc gggccgtgcg cgtgcgtcag cccccgagcg cggcgctgac
     3241 cacc
//
LOCUS       DATMSX010000547         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_122491,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000547 DATMSX010000000
VERSION     DATMSX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122491"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1337)
                     /locus_tag="VN041_17350"
     CDS             complement(45..1337)
                     /locus_tag="VN041_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30850.1"
                     /translation="MNRRILAVGSIAAVAALALAGCSGSTEADKPAASSGPVTLTMSA
                     WSVDTTPEFKALADAFHEKNPDITINLKEYDATDYNTLITADLAAGSGPDIITQKEVK
                     YVTTLQEGGQLLDVSDVKLPKGIGGADSYKVDGKAYATPYRQDSWVLFYNKDLFKKAG
                     VAEPDGSWTWDDFDKAAAELTAGAGGAKGAYLHRWQSVVQGFANSQTGSNVLKGKYDY
                     MTPFYERVLKLQKDGDVVDFNTSSANQLTYQGEFGKQKAAMMPMGTWFVATLIAQQAS
                     GDADAFSWGIAPIPQLSAKTAGLDNTPVTFGDPTGFGVNAAASPEKAAAAKKFLAFAS
                     SEDAAQVLAGIGITPALNSSTVADTYFGVKGAPTDDLSKFAWSTHKVLAENPTSSKTA
                     AIQGILSDMHTAIMSGSTPVDAAIKEAQDRVSNEVGLD"
     gene            1336..>1602
                     /locus_tag="VN041_17355"
     CDS             1336..>1602
                     /locus_tag="VN041_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30851.1"
                     /translation="MLFVLLRDCGLAAVRVSQSSFEGSRLVVRRPSGQTTVAASASRV
                     RGLPTPVEGRDRPPLFRPMVDHFEIFLDHVTASAQDEHPSALPVP"
BASE COUNT          199 a          519 c          571 g          313 t
ORIGIN      
        1 gcgggaagcg gggccggcct ccgcggccgg ccccgcgcag cgggtcagtc caggccgacc
       61 tcgttggaga cgcggtcctg cgcctccttg atcgcggcgt cgaccggcgt ggagccggac
      121 atgatcgccg tgtgcatgtc gctcaggatg ccctggatcg ccgcggtctt gctcgaggtg
      181 gggttctcgg cgagcacctt gtgcgtcgac catgcgaact tcgacaggtc gtcggtcggt
      241 gcgcccttga ctccgaagta ggtgtcggcg acggtgctgc tgttcagcgc cggggtgatg
      301 ccgatgccgg cgagcacctg cgcggcgtcc tcgctggacg cgaaggcgag gaacttcttc
      361 gcggccgcgg ccttctccgg gctggcggcg gcgttgacgc cgaagccggt cgggtcgccg
      421 aaggtgaccg gggtgttgtc gagccccgcc gtcttcgcgc tgagctgcgg gatcggcgcg
      481 atgccccacg agaaggcatc cgcgtcgccc gaggcctgct gcgcgatgag cgtggcgacg
      541 aaccaggtgc ccatcggcat catcgcggcc ttctgcttgc cgaactcgcc ctggtaggtg
      601 agctggttgg cgctggaggt gttgaagtcg acgacgtcgc cgtccttctg cagcttcagc
      661 acgcgctcgt agaacggcgt catgtagtcg tacttgccct tgagcacgtt cgacccggtc
      721 tgcgagttgg cgaagccctg aacgaccgac tgccagcggt gcaggtaggc gcccttcgcg
      781 ccgccggcgc cggcggtcag ctcggcggcg gccttgtcga agtcgtccca tgtccacgag
      841 ccgtcaggct cggcgacgcc ggccttcttg aacaggtcct tgttgtagaa gagcacccag
      901 gagtcctgcc ggtacggggt cgcgtacgcc ttgccgtcga ccttgtagga gtcggcgccg
      961 ccgatgccct tgggcagctt cacgtcggag acgtcgagca gctggccgcc ctcctgcagg
     1021 gtggtgacgt acttgacctc cttctgcgtg atgatgtcgg ggccggagcc ggcggcgagg
     1081 tcggcggtga tcagcgtgtt gtagtcggtc gcgtcgtact ccttcaggtt gatcgtgatg
     1141 tcggggttct tctcgtggaa cgcgtccgcg agcgccttga actccggcgt ggtgtcgaca
     1201 ctccatgccg acatcgtgag ggtgacgggt ccgctggaag ctgcgggctt gtcggcctcg
     1261 gtgctgccgc tgcatccggc gagcgccagc gctgccacag cggcgatgct gccgacggcg
     1321 aggatccttc tgttcatgct gttcgtgctc cttcgtgatt gcggtctggc cgcggttcgg
     1381 gtgtctcagt cttcgttcga ggggagtcgg ctggtggtgc gcaggccgtc cggccagacg
     1441 accgtcgccg catcggcttc gagggtgagg ggcctgccca cgccagtcga aggccgtgac
     1501 cgcccacccc tgttccgccc aatggtggat cacttcgaga tatttctcga tcatgtcacc
     1561 gcgtccgccc aggacgagca tccgtccgcg ctccccgtcc cc
//
LOCUS       DATMSX010000548         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_122492,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000548 DATMSX010000000
VERSION     DATMSX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122492"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /gene="mltG"
                     /locus_tag="VN041_17360"
     CDS             <1..1251
                     /gene="mltG"
                     /locus_tag="VN041_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWU30852.1"
                     /translation="EEPADPAASAPRAADPVASTLPGPAASDPEPDHDDPSQDEPEPG
                     GSLDALFAPEQHRSVPKKKRGRGCLIGLIVVLVVLGGIAAGGAWVYNTYQDKINDMMG
                     WGEPKDYAPGEATGEVQVTILKGDTGSPVSTTLYKAGVTKTDRVFYEYLLAERPTVTF
                     YPGVYKLQKKMTAAAALKALDDPKNRMANTVRIAEGSTVESSLPRIVQGVGLKLEDLQ
                     AAVQDPSVYGVQAPRLDGWLFPATYTFDPGVTAKEVIQRMVDETRAQLKKAGVPDSDA
                     QRVLTIASIVEREGRTPDFAKVSRVIQNRLDTGMMLQMDSTAQYGYGQLHAGSASTSK
                     EAQHAENEWNTYVIKGLPATPIANPGARAIDAAMHPADGSWLYFVTVNMSTGETVFSN
                     TYAEHQQAIKQMQAWCKANPGTGC"
     gene            1253..>1660
                     /locus_tag="VN041_17365"
     CDS             1253..>1660
                     /locus_tag="VN041_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWU30853.1"
                     /translation="MSTRSRLAVWGDPIGHSRSPALHGAAYAQLGLDWQYGRQRVDVA
                     GFPAALNALDDSWRGLSLTMPLKEIAHDSAAELDGHARLTGAVNTLLLGERLRGFNTD
                     VGGIVDAFRNAGVSGIRSVRILGAGATAASALVA"
BASE COUNT          283 a          570 c          578 g          229 t
ORIGIN      
        1 gaggagcctg ctgaccccgc cgcttccgcg ccgagagcgg ccgacccggt ggcatccacg
       61 ttgccgggcc ccgctgcgtc ggacccggag cccgaccacg acgacccgtc gcaggacgaa
      121 cccgaacccg gcggttcgct cgacgccctg ttcgcgcccg agcagcaccg ctcggtgccg
      181 aagaagaagc gcggacgcgg ctgcctgatc gggctgatcg tcgtgctggt cgtgctcggc
      241 ggcatcgccg ccggaggcgc gtgggtgtac aacacctacc aggacaagat caacgacatg
      301 atgggctggg gcgagcccaa ggactacgcc ccgggcgagg cgaccggcga ggtgcaggtc
      361 accatcctga agggcgacac cggttctccg gtgtcgacga cgctgtacaa ggccggtgtc
      421 accaagaccg atcgcgtctt ctacgagtac ctgctggcgg agcggccgac ggtcacgttc
      481 taccccggcg tgtacaagct gcagaagaag atgaccgcgg ctgccgccct gaaggctctg
      541 gacgacccga agaaccgcat ggccaacacc gtgcggatcg ccgagggcag caccgtcgag
      601 tcgtcgctgc cgcgcatcgt ccagggggtc ggtctgaagc tcgaggacct tcaggcggcc
      661 gtgcaggacc ccagcgtcta cggcgtgcag gcgccgcggc tggacggctg gctgttcccg
      721 gcgacctaca cgttcgatcc cggcgtcacg gcgaaggagg tcatccagcg gatggtcgac
      781 gagacgcgtg cgcagctgaa gaaggccggt gtcccggatt ccgacgctca gcgcgtgctg
      841 accatcgcct cgatcgtgga gcgcgagggc cgcacgcccg acttcgcgaa ggtgtcccgg
      901 gtgatccaga accggctcga cacgggcatg atgctgcaga tggactccac cgcgcagtat
      961 ggctacgggc agctgcacgc cggaagtgcg agcacctcga aggaggcgca gcacgccgag
     1021 aacgagtgga acacgtacgt catcaagggg ctgccggcga cgccgatcgc caaccccggt
     1081 gctcgtgcga tcgacgccgc catgcatccc gccgatggca gctggctcta cttcgtgacg
     1141 gtgaacatga gcacgggcga gaccgtcttc tcgaacacct acgccgagca ccagcaggcc
     1201 atcaagcaga tgcaggcatg gtgcaaggcg aaccccggca caggatgctg aggtgagcac
     1261 gcgctctcgt ctggccgtct ggggcgaccc gatcggccac agccggtcgc ccgccctgca
     1321 cggtgccgcc tacgctcagc tggggctgga ctggcagtac ggccggcagc gcgtcgacgt
     1381 ggcgggtttc cccgccgcgc tgaacgcgct ggacgactcg tggcgcggac tctcgctgac
     1441 catgccgctc aaggagatcg cgcacgactc ggcagccgaa ctggacggac acgcccgcct
     1501 gaccggagcg gtcaacacgc ttctgctggg ggagcggctg cgcggcttca acaccgatgt
     1561 cggcggcatc gtcgacgcgt tccggaacgc cggcgtctcc ggcatccggt cggtgcggat
     1621 cctcggtgcg ggggcgaccg cggcatccgc gctcgtcgcc
//
LOCUS       DATMSX010000549         7406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_17503,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000549 DATMSX010000000
VERSION     DATMSX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17503"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VN041_17370"
     CDS             complement(<1..408)
                     /locus_tag="VN041_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30854.1"
                     /translation="MNGIAEALTARERAAQEYLTENGWTQGDFKNNVGEVCLHGAVMY
                     CQPQNGDDQIITQVLNRRGFDYDWNDTIGREPAEVIEALSADITDAELQDVFGPQWQE
                     IVALIRRARVITPTEADAARDAARDAARDAARAA"
     gene            complement(405..584)
                     /locus_tag="VN041_17375"
     CDS             complement(405..584)
                     /locus_tag="VN041_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30855.1"
                     /translation="MTELISTLEAATMLRKSVRTVHRMISRGDLPATKLPGATGAYVL
                     ERSAVEIERSKGTAG"
     gene            complement(957..1029)
                     /locus_tag="VN041_17380"
     tRNA            complement(957..1029)
                     /locus_tag="VN041_17380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Arg,seq:ccg)
     gene            complement(1075..1515)
                     /locus_tag="VN041_17385"
     CDS             complement(1075..1515)
                     /locus_tag="VN041_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWU30856.1"
                     /translation="MSSSDLNGLYQELILDHSRAPYGYGLREPIAAQSHQLNPTCGDE
                     ITLQMHRAPDGTIEAVAWEGHGCAISQASASLFAELVEGMPEDEVIRRIDAFREAMRS
                     RGRIEPDPELLGDATALGGVSKYVARVKCAMLAWVAAEDALRKG"
     gene            complement(1515..2813)
                     /locus_tag="VN041_17390"
     CDS             complement(1515..2813)
                     /locus_tag="VN041_17390"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWU30857.1"
                     /translation="MHNTASIVDTLTEAEVLRLREDFPILQTEVNGHPLVYLDSGATA
                     QRPLAVLDAERDYLLNLNAAVHRGAHTLAAEATDAFEDARETLARFVGADADEIVWTS
                     NATEAVNLVAYSLSNASAGRGGAEADRLRLAEGDEIVTTEMEHHANLIPWQELAARTG
                     ATLRVIPLDDEGALRMEEAARLIGPRTRIVAVTHVSNVLGVVNPIEDVIALAREVGAM
                     VLLDACQSAPHLPLDLHALDVDFAVLSGHKMLGPTGIGALYGRRELLEAMPPFLTGGS
                     MITTVTTTEAAYLPPPQRFEAGTQRVSQAIALAAAVRYLERVGMPRIAAHEAELGSRL
                     VDGLAAIDGVRVLGSGIARPRVGLASFDVAGIHAHDVGQILDDEGIAVRVGHHCAQPL
                     HRRLGITASTRASTYLYTTRDEVDAVIAGVARAIDFFRRR"
     gene            complement(2887..3756)
                     /locus_tag="VN041_17395"
     CDS             complement(2887..3756)
                     /locus_tag="VN041_17395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWU30858.1"
                     /translation="MTAVAWRRLGPAALVCLAAPAFFVLGWTWVGWVLIAIGVGTAAL
                     VERRPPESGGAVFAGHRPTWRIGERRAPSLARDLVLIAAGMLIVHAISLEAQLDDAAM
                     LRFTLALGGAVLVPYLVSRFVFRDRAISFPWRTRRRWARWQWAWLVAVLVLGWLILPY
                     YFISSGVYRNWPVVDSPELIGRLFVGVGAVGIWDELFFICTVFALLRRHFPDMTANLL
                     QAIVFVSFLWELGYRAWGPVLTVPFALLQGWIFLRTHSLAYVVTVHLLFDAVVFAVLV
                     HAHNPGLLPVFLI"
     gene            complement(3753..4478)
                     /gene="trmB"
                     /locus_tag="VN041_17400"
     CDS             complement(3753..4478)
                     /gene="trmB"
                     /locus_tag="VN041_17400"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWU30859.1"
                     /translation="MPEPRTFREEPVSFVRRSGRMSEGQERAFEELAPSYLIDVPRDV
                     AFTSVHPDARLDVASVYGRTAPLIVEIGSGQGHAIVSAASSRPDDDFLAVEVFRAGLA
                     RTMLDADREGARNLRLVEANAPEVLSTFLPEGAASEVWIFFSDPWHKSRHNKRRMIRP
                     GFPPIAARALADGGLLRLATDWEDYALQMREVLDEAPEFERAFDGEWAERFDGRVMTA
                     FERKGIAKGRDIRDLTYRRVLRV"
     gene            complement(4538..5377)
                     /locus_tag="VN041_17405"
     CDS             complement(4538..5377)
                     /locus_tag="VN041_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HWU30860.1"
                     /translation="MDDRYGSDVLAAGWREHGFAKASDVPAVKDLVVEVAADGYCGAI
                     TRVEAGMVELEDWKGRRRSFPMGGGFLVEGRPVRLVVPKAAPQGTRRTASGSFVVADD
                     RAKVALPSRILVEGRHDAELVEKVWGADLRVEGVVVEYLQGIDLLDELLTEAPPNATR
                     RYGVLVDHLVPGSKETRMVEQIIRGPHGKHLKIVGHPYIDVWQCVTPAAMGIRAWPQI
                     PRGTDWKTGICRAFGWPAEDQADIARAWQRILSRVTTFRDLEPSLLGRVEELIDFVTA
                     PAS"
     gene            5501..>7406
                     /locus_tag="VN041_17410"
     CDS             5501..>7406
                     /locus_tag="VN041_17410"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWU30861.1"
                     /translation="MRQRWLVWVAVLLGAVLGGFSLSAASATAPGPLDGHITDRSGVV
                     SSSDRAQAESTLEKLRTEAGIDLFFVMIPEYTDPASMEQWTTATAQGNSFRGDQYLIS
                     VATEGRSYTMYRPDAGRMTTSMRDRILSAMYPDLAASDFSHAIVAGADEAYDIYVLGP
                     QRAATGWTVFGIIVLIAVIIVVVVLLIRRARKRAAEQAKRRAQLAEIGQQANIALVRT
                     DDLVRSSEQEMEYARAQFGDDVIGDFVTALQTSRTNLNEAFSLQQKFDDEVPDTDEQK
                     LEWSQRILELCSESTKVLEERKADFDELRKLEQNAPAALDNVRKLRSAAGAEVDRADQ
                     ILAALTAAYASAAISPVADNPAQARSRLAFTDTQVEAADGFIAAGKTGDAAVSVRAAE
                     GAVQQATQLEDAVEQLQKSLKDADDRAAALIAELGSDLQTARTLPDAQGAVAQAVAAT
                     EQGIRQAQSQLGASGRNPIEALRVLEAANIAIDGVIAHVRDEQARIQRAAAMLGDAMQ
                     RAQMQISSAESFILNRRGSIASTARTRLSEAQGSLDQAQSLAGTDPVQALALAQRADT
                     LAAEALRLAQNDYGGWSGGGGGNDNLGAFIGGVLIGSAGNHHGGGGGWGDGGIFGGGG
                     GGGFSGGGSGG"
BASE COUNT         1197 a         2612 c         2517 g         1080 t
ORIGIN      
        1 ggcggccctc gcggcgtccc tcgcggcgtc cctcgcggcg tccctcgcgg cgtccgcctc
       61 agtcggcgtg atgacgcgag cgcggcggat gagcgccacg atctcctgcc actgcggacc
      121 gaacacgtcc tgcagctccg cgtccgtgat gtccgcactg agcgcctcga tcacctcggc
      181 gggctcacgc cctatcgtgt cgttccagtc gtagtcgaaa ccgcgacggt tcagcacctg
      241 agtgatgatc tgatcatcac cgttctgcgg ctggcagtac atcacagcac cgtgcagaca
      301 cacctcgccg acgttgttct tgaaatcgcc ctgcgtccac ccgttctccg tcagatactc
      361 ctgcgccgca cgctcacgcg ccgtcagcgc ctcagcaatc ccgttcatcc tgccgttccc
      421 ttcgatcgct cgatctcgac agcagagcgc tccagcacgt acgcgccggt ggcaccgggc
      481 agtttcgtgg ctggcaggtc gccgcgcgag atcatccggt gaaccgtccg cacgctcttg
      541 cgaagcatcg tcgccgcttc gagcgttgat atcagttcgg tcatatgacc aagttaggtt
      601 gttatccctt attggtcaaa tacccaggat cagcgtgttg acgcgagcgc gctaagtgac
      661 ctagtttgga cgtatgagca tgcaaagtgc agcgggcatc cgcccctcgt tcacgatcgg
      721 cgaccgcttg cggaaggcgc ggtcactcct cggtccggat atggatgtca agcgcttctc
      781 cgagctgctc acggtgagca agaacaccgt gacgaactac gagctggaga acaccgaccc
      841 ggcgcgtatg aagccgattg tgcttcgaca gtgggcgctc gcgaccggcg tcgactatga
      901 atggctgctt aacggcgaaa ccccgcccac tggggacggg gtttcgccgg atggtgtgcc
      961 cccgacagga atcgaacctg cgacctacgg taccggaaac cggcgctcta tccgctgagc
     1021 tacggaggcg aactcatcga gcctatcatt cagcggagcc cgtcccgttc cgcctcaccc
     1081 cttgcgcaga gcatcctccg cggcgaccca ggcgagcatc gcgcacttca ccctggcgac
     1141 gtacttcgac actcccccca gggcggtcgc gtccccgagc agctcgggat ccggctcgat
     1201 ccggcccctc gatcgcatcg cctcgcggaa cgcgtcgatc cgcctgatca cctcgtcctc
     1261 gggcatcccc tcgacgagct cggcgaacag cgacgccgag gcctgcgaga tcgcgcagcc
     1321 gtggccctcc caagcgacgg cctcgatcgt gccgtccggc gcgcggtgca tctgcagggt
     1381 gatctcgtcg ccgcaggtgg gattgagctg gtgggactgg gcggcgatcg gctcgcgcag
     1441 cccgtagccg tacggcgcac gggagtgatc gaggatgagc tcctggtaga gcccgttgag
     1501 atcggaagag gacatcagcg cctccggaag aagtcgatcg cgcgggcgac ccctgcgatc
     1561 accgcgtcga cctcgtcgcg ggtcgtgtac aggtaggtgc tggctctggt ggatgccgtg
     1621 atgcccaggc gccggtgcag cggctgagcg cagtgatggc ccacgcgcac cgcgatgccc
     1681 tcgtcgtcca ggatctgacc gacgtcgtgg gcgtggatgc cggcgacgtc gaagctcgcg
     1741 agcccgacgc ggggacgtgc gatgcccgat cccagcactc gcacgccgtc gatcgcggcg
     1801 agcccgtcga cgagtcgaga tcccagctcg gcttcgtgcg cggcgatccg cggcatcccg
     1861 acgcgctcca ggtagcggac ggcggcggcc agcgcgatcg cctgcgagac gcgctgcgtt
     1921 ccggcctcga agcgctgcgg cgggggcaga tacgcggcct cggtggtcgt gacagtcgtg
     1981 atcatcgaac cgccggtgag gaacggtggc atggcctcga gcagctcccg ccgcccgtac
     2041 agggcgccga tcccggtcgg gccgagcatc ttgtgcccgg acagcaccgc gaagtccacg
     2101 tcgagagcat gcaggtccag aggcaggtgg ggcgcggact ggcaggcatc cagaagcacc
     2161 atcgccccga cctcgcgggc gagcgcgatg acgtcctcga tcgggttgac cacgccgagg
     2221 acgttcgaca cgtgggtgac ggcgacgatc cgggtgcgcg gcccgatcaa ccgggcggcc
     2281 tcctccatgc gcagcgcgcc ctcatcgtcc agtgggatga cgcgcagcgt cgcgccggtg
     2341 cgcgccgcca gctcctgcca cgggatgagg ttggcgtggt gctccatctc ggtggtcacg
     2401 atctcgtcgc cctcggcgag gcgaagcctg tccgcctccg ctccgccacg acccgccgag
     2461 gcgttcgaca gcgagtacgc gacgaggttc acggcctcgg tggcgttgga cgtccagacg
     2521 atctcgtccg catcggcgcc cacgaagcgc gccagggtct cacgcgcgtc ctcgaaggca
     2581 tccgtcgcct cggcggcgag cgtgtgcgcg ccccggtgca cggccgcgtt gaggttcagg
     2641 aggtagtcgc gctcggcgtc gagcaccgcg agcggccgtt gcgcggtcgc gccggagtcg
     2701 aggtagacga gcggatgccc gttcacctcg gtctgcagga tcgggaagtc ctcgcggagg
     2761 cggagcacct ccgcttcggt gagcgtgtcc acgatggagg cggtgttgtg cacccctcca
     2821 gtctcccacc tgcgaggacc gagacggccg tcgtgcctca ggagctgtcg tgcccccggg
     2881 gcgacctcag atcaggaaca ccgggagcag gcccgggttg tgggcgtgca cgaggacggc
     2941 gaacacgacc gcgtcgaaca gcagatgcac cgtgacgacg taggcgagcg aatgcgtgcg
     3001 caggaagatc cagccctgca gcagggcgaa cgggacggtc agcactggcc cccacgcccg
     3061 gtaaccgagc tcccacagga acgacacgaa cacgatcgcc tgcagcaggt tcgcggtcat
     3121 gtcggggaag tgcctccgca gcagcgcgaa caccgtgcag atgaagaaca gctcgtccca
     3181 gatgcccacc gctcccacac cgacgaagag ccggccgatc aactccggcg agtcgacgac
     3241 cggccagttg cggtacacgc cgctggagat gaagtagtac ggcaggatca gccagccgag
     3301 caccagcacg gccaccagcc acgcccactg ccagcgcgcc cagcgcctgc gagtgcgcca
     3361 tgggaacgag atcgcccgat cccggaacac gaaccgcgag acgaggtacg gcacgagcac
     3421 cgctcccccg agcgccaggg tgaatcgcag catcgccgcg tcgtcgagct gagcctccaa
     3481 cgagatggcg tgcacgatca gcatcccggc cgcgatcagc acaagatccc gcgcgagcga
     3541 cggcgcgcgt ctttcgccga tgcgccacgt cggccggtga ccggcgaaca ccgctccgcc
     3601 gctctcgggt ggccggcgct ccaccagggc ggcggttccg acgccgatcg cgatcagcac
     3661 ccatccgacc caggtccagc cgagcacgaa gaaggccggc gccgcgaggc acaccagtgc
     3721 ggcgggcccg agccgccgcc aggcgacggc agtcacaccc gcagaacccg gcggtaggtc
     3781 aggtcccgga tgtcgcggcc cttggcgatg cccttgcgct cgaaggcggt catcactcgg
     3841 ccgtcgaaac gctcggccca ttcgccgtcg aaggcgcgct cgaactcggg cgcctcgtcg
     3901 agcacctcgc gcatctgcag cgcgtagtcc tcccagtcgg tcgccagccg cagcagcccg
     3961 ccatcggcga gagcgcgtgc ggcgatgggc gggaatccgg ggcggatcat gcggcgcttg
     4021 ttgtgacgtg acttgtgcca cgggtcggag aagaagatcc acacctcgga tgccgcgccc
     4081 tccggcagga acgtcgacag cacctccggc gcgttcgcct ccaccagacg caggttgcgg
     4141 gcgccctcgc gatccgcgtc gagcatcgtc cgggccaggc cggcgcggaa gacctcgacg
     4201 gcgaggaagt cgtcatcagg gcgcgacgac gccgcggaca cgatcgcatg tccctggccg
     4261 gatccgatct cgacgatcag cggggcggtg cggccataga cggatgccac gtccagccgg
     4321 gcatccggat gcaccgaggt gaacgccacg tcacgcggga cgtcgatcag ataggacgga
     4381 gcgagctcct cgaacgcgcg ctcctgaccc tcggacatgc ggccgctgcg gcgcacgaac
     4441 gagaccggtt cttcgcggaa ggtgcggggt tctggcatcc ctccagggta ccggggcgag
     4501 ccctcccgcc ccgtcgggat gccgcgcgac cggccgctca tgatgcgggc gcggtgacga
     4561 agtcgatcag ctcctccacc cgccccagca gggagggctc gaggtcgcgg aaggtcgtca
     4621 cgcgggacag gatgcgctgc cacgcccgcg cgatgtcggc ctgatcctcc gcgggccagc
     4681 cgaacgcacg gcagatcccc gtcttccagt cggtgccgcg cgggatctgc ggccaggcac
     4741 ggatgcccat cgcggccggc gtgacgcact gccagacgtc gatgtagggg tggccgacga
     4801 tcttgaggtg ctttccgtgc gggccgcgaa tgatctgctc gaccatccgg gtctccttcg
     4861 acccgggcac gaggtggtcg accagcacgc cgtagcggcg ggtcgcgttc ggcggcgcct
     4921 cggtcaacag ctcgtcgagc aggtcgatgc cctgcaggta ctcgaccacg acgccctcga
     4981 cccgcaggtc ggcgccccac accttctcca ccagctcggc gtcatgccgg ccctcgacca
     5041 ggatgcggct gggaagcgcg accttcgcgc ggtcgtcggc gacgacgaac gagccggatg
     5101 ccgtgcgtcg agtgccctgc ggcgcggcct tcggcactac gaggcgcacg ggcctgccct
     5161 ccacgaggaa gccaccgccc atcgggaagc tgcgtcggcg ccccttccag tcctccagct
     5221 cgaccatccc ggcttcgacg cgggtgatcg ctccgcagta gccgtccgct gccacctcga
     5281 cgacgagatc cttcacggcg ggcacgtcgc tcgccttcgc gaagccgtgc tcccgccatc
     5341 ccgccgcgag cacatcgctg ccgtacctgt cgtccatgcc ttccagcgta tgcgcctccg
     5401 cggacagcga acgggcagcg cccagcggat gctgtcgcgt gtcggccgct gtgcatagac
     5461 tcgtcgcatg ggggcacggc tgccggtcgg agggaacagt atgcgtcaac ggtggctggt
     5521 ctgggtcgcg gtcctgctcg gcgccgtcct gggcgggttc tcgctgagcg cggcctccgc
     5581 cacggccccg ggtccgctcg acgggcacat caccgaccgc agtggcgtcg tgtcgagctc
     5641 cgatcgcgcg caggcggaga gcacgctcga gaagctgcgc accgaggccg gcatcgatct
     5701 gttcttcgtg atgatcccgg agtacaccga ccccgcctcc atggagcagt ggaccaccgc
     5761 cacggcgcag ggcaacagct tccgcggcga tcagtacctg atctccgtgg caacggaggg
     5821 ccgctcctac acgatgtacc gtccggacgc cgggcggatg accacctcga tgcgagaccg
     5881 gatcctctcc gcgatgtacc ccgacctggc ggcgagcgac ttcagccatg ccatcgtcgc
     5941 gggggcggac gaggcgtacg acatctatgt cctgggtcct cagcgtgctg cgacggggtg
     6001 gacagtgttc ggcatcatcg tgctgatcgc cgtgatcatc gtcgtcgtcg tgctgctgat
     6061 ccgacgggcc cgcaagcgcg ctgccgaaca ggcgaagcgc cgcgcgcagc tcgcggagat
     6121 cggccagcag gcgaacatcg cgctcgtgcg caccgacgat ctcgtgcgct ccagtgagca
     6181 ggagatggag tacgcgcgcg cccagttcgg cgatgacgtg atcggtgact tcgtcacggc
     6241 gctgcagacc tctcgcacca atctcaacga ggccttctcc ctgcagcaga agttcgacga
     6301 cgaggtcccc gacaccgacg agcagaaact ggagtggtcg cagcgcattc tcgaactgtg
     6361 ctccgagtcg acgaaggtgc tcgaggagcg caaggccgac ttcgacgaac tgcggaagct
     6421 ggagcagaac gcccccgccg cgctggacaa cgtgcgcaag ctccgatccg ccgccggtgc
     6481 cgaggtcgat cgcgccgatc agatcctcgc cgcgctcacg gccgcctatg cgtccgcggc
     6541 gatctcaccc gtcgcagaca accccgcgca ggcccgatcc cgcctcgcgt tcaccgacac
     6601 gcaggtcgag gcggccgacg ggttcatcgc cgcgggcaag accggtgacg cggccgtctc
     6661 ggtgcgcgcc gcggagggcg ccgtgcagca ggcgacgcag ctggaggatg ccgtcgagca
     6721 gttgcagaag tcgctgaagg atgccgatga tcgtgccgcg gccctcatcg ctgagctggg
     6781 cagcgacctg cagacggccc gcacgctgcc cgatgcacag ggcgctgtcg cccaggccgt
     6841 cgcggccacg gagcagggca tccgccaggc gcagagtcag ctcggcgcat cgggtcgcaa
     6901 tcccatcgag gcgctgcgtg tcctggaggc ggcgaacatc gcgatcgacg gcgtcatcgc
     6961 gcacgtgcgc gatgagcagg ccaggatcca gcgcgccgcc gccatgctgg gcgatgcgat
     7021 gcagcgtgcg cagatgcaga tctcgtcggc ggagagcttc atcctcaatc gccgcggctc
     7081 gatcgcctcg accgcgcgca cacggctctc cgaggcccag ggaagcctcg accaggcgca
     7141 gagcctcgct gggacggatc cggtgcaggc cctcgcgctc gcgcagcgcg ccgacaccct
     7201 cgccgccgag gccctgcgcc tcgcccagaa cgactacggc gggtggagcg gcggcggagg
     7261 cgggaacgac aatctcggcg ccttcatcgg cggcgtcctc atcggctcgg ccggcaacca
     7321 ccacggcggt ggcggcggct ggggagatgg cggcatcttc ggcggtggcg gaggcggagg
     7381 cttcagcggc ggcgggagcg gcggag
//
LOCUS       DATMSX010000550         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_94156,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000550 DATMSX010000000
VERSION     DATMSX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94156"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VN041_17415"
     CDS             complement(<1..335)
                     /locus_tag="VN041_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWU30862.1"
                     /translation="MRAVVYPRFSQDLDTLEVREVPEPKLSPASVIIEVRAAGVNPVD
                     WKLMAGGLDALMPTQFPVIPGWDVAGVVVAVGLDTPEFQVGDEVMAYARTDTVGGGTF
                     AERVDVPVRA"
     gene            complement(423..>1593)
                     /locus_tag="VN041_17420"
     CDS             complement(423..>1593)
                     /locus_tag="VN041_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HWU30863.1"
                     /translation="PRRRTTSGIRRSGTSASRRSTASPVPASDCRRGSNAQTYRIFTR
                     LDEHDRPGVGLNLTAAVRAAVLKYPWRRGEGGARRGGASKFNFYAIDEQDARGALSAY
                     PLIEPGQQTVECSIMDISDDIAYSLHDLDDFYRAGLLNPATLSAEFRTWHRELATLRA
                     SDVAALAADSRMPGSSLELLWRRLKEKDAWIADPDAFSEAVAKVKTEVIDGLVAEPFD
                     GSLASERTLARFTSGWIARLQSSVEVHRHPHIRCGHVSLNRQAWHEVAVLKFLHERFI
                     LERPDLTVYQRGQASVLERLVDGFTAWLDDPRDARRAPRRLIDLVDLSTDDYRRLRKD
                     APELVESRTDADLDALARGRGIIDYVASLTDAQAISLDALLAGRTERLWDAGQGL"
BASE COUNT          234 a          550 c          582 g          227 t
ORIGIN      
        1 gcccgcacgg gcacgtcgac gcgctcggcg aacgtgccgc cgccgaccgt gtcggtgcgc
       61 gcgtaggcca tcacctcgtc gccgacctgg aactcggggg tgtccagccc cacggccacg
      121 accacgccgg cgacatccca accggggatc acggggaact gcgtgggcat gagggcgtcc
      181 aggccgccgg ccatgagctt ccagtccacc gggttcacgc ccgcagcccg cacctcgatg
      241 atcacggatg ccggagacag cttcggctcg gggacctcac gcacctcgag ggtgtcgaga
      301 tcctggctga aacggggata gacgacggcg cgcatgctga cctcctgagg gtgtgtccgt
      361 ccccactctc gcacgcgtgc gcacccatcg ggccgaagtt ccgggatgag gaacgccccc
      421 ggctacagtc cctgcccggc gtcccacagc cgctcggtgc ggccggcgag cagcgcatcc
      481 agcgagatcg cctgcgcgtc ggtgagcgag gcgacgtagt cgatgatgcc gcggccgcgc
      541 gcgagcgcat cgagatcggc atccgtgcgc gactcgacca gttccggcgc gtccttccgc
      601 agccgccggt agtcgtcggt ggacaggtcg accaggtcga tcagccgtcg cggggcgcgg
      661 cgggcgtccc gcggatcgtc gagccaggcg gtgaagccgt cgaccagacg ctccagcacg
      721 ctggcctgac cgcgctggta gacggtgagg tcggggcgct ccaggatgaa ccgctcgtgc
      781 aggaacttca gcaccgcgac ctcgtgccag gcctgccggt tcagcgagac gtggccgcag
      841 cggatgtgcg gatgccggtg cacctccacc gacgactgca ggcgcgcgat ccagccgctg
      901 gtgaacctcg ccagggtgcg ctcgctcgcc agggagccgt cgaacggctc ggcgacgagc
      961 ccgtcgatga cctctgtctt gaccttcgcg accgcctcgc tgaaggcgtc ggggtcggcg
     1021 atccaggcgt ccttctcctt cagccgtcgc cagaggagtt ccagcgagga gcccggcatg
     1081 cgggagtcgg cggccagcgc cgcgacgtcg gatgcccgca gcgtcgcgag ttcgcggtgc
     1141 caggtgcgga actcggccga cagggtcgcg gggttgagca gaccggcacg gtagaagtcg
     1201 tcgaggtcgt gcagcgagta ggcgatgtcg tcggagatgt ccatgatcga gcattcgacg
     1261 gtctgctgac ccggctcgat cagcgggtac gccgagaggg cgccgcgggc atcctgctcg
     1321 tcgatcgcgt agaagttgaa cttgctcgcg ccgccccgcc gtgctccgcc ctcgccgcgc
     1381 cgccagggat acttcagcac ggcggcgcgc acggccgcgg tgaggttgag gccgacgccg
     1441 gggcggtcgt gctcgtccag gcgggtgaag atgcggtagg tctgcgcgtt cgaacccctc
     1501 ctgcagtccg aagcgggtac gggcgaggcg gtcgagcgcc tgctcgccga ggtgcccgaa
     1561 cggcggatgc ccgaggtcgt gcgccgccga ggc
//
LOCUS       DATMSX010000551        11970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_122496,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000551 DATMSX010000000
VERSION     DATMSX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122496"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VN041_17425"
     CDS             <1..480
                     /locus_tag="VN041_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWU30864.1"
                     /translation="NAETHATVALAPQSAGGRDLDVVRLALHEPGAIARANAAVEEEK
                     DEEAGVVVDISRKRVLLDGESAAFTYKEFELLQYLVLREGRTIERSELVSALWQASTD
                     EDAPGERTIDVHVRRLRAKLGRHEDIVRTVRGVGYRFDRHADVSIRYGHGTPSPDRF"
     gene            562..1509
                     /locus_tag="VN041_17430"
     CDS             562..1509
                     /locus_tag="VN041_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HWU30865.1"
                     /translation="MTLTDAAPTAHAVPRESVHRPRHPLDLMATVGMLQRGRNDPTMV
                     VEGGRIWMAFRTGAGVATLCLRATSGGVHATAWGPGAAEALDRVPALCGADDDQSGFD
                     VSGHPRLTEVARRNPGIRLTRTGRVFDALTCAIIEQKVTGMQAFGAWRHLVSRNGERA
                     PGPTPRPMFAAPHSEVWRAIPSWGFHRAGLEPPQARTIARAAMRGPSIERAVLAASDG
                     DGRERVLTSLPGIGPWTSAETRIRALGDPDAVSVGDFHLAHEVGFALTGSRTDDDGML
                     ELLAPWAGHRQRIIRLIFASGVREPRRGARLAPEDHRSR"
     gene            complement(1522..2280)
                     /locus_tag="VN041_17435"
     CDS             complement(1522..2280)
                     /locus_tag="VN041_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30866.1"
                     /translation="MIRLSNVIKRYSGDVEIGPISIDLPAGGIIALVGPNGAGKSTVL
                     TMMGRLLAPDSGTVEVGGLDVHRMPSKQVARTVSILRQENHFVTRLTVRQLVGFGRYP
                     YSGGRLTQRDEDKISEAIDFLNLTDLEQRFLDQLSGGQRQRAYVAMVLAQDTEYLLLD
                     EPLNNLDAQHSVQMMRQLRRAADRLGRTIVVVLHDINFAAAYSDYIVAMESGRIAVHG
                     TPEEIIRDEVLTAVFHTPVTVVDGPGGKFAVYHR"
     gene            complement(2277..3350)
                     /locus_tag="VN041_17440"
     CDS             complement(2277..3350)
                     /locus_tag="VN041_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWU30867.1"
                     /translation="MRSDVSFTPAPMSATPLGASAPSSDRRVGPFSGRAHRRRYLLIL
                     AGLIVASLALVVLVLTWGNSQIPFTERWWRVSRMRSESLIVIALVTLCQSFATVSFQT
                     VTGNRILSPSIMGFESLYILIQTAVVFFFGVSGLAEIDGFAQFVIQAAGMVVFAVLLY
                     SWLFSGRLGNLHVMLLVGIIIGTGLGALSAFMQRMLDPNEFDVLRARMFGNVGNANTE
                     YLWFVIPVCVGAAGGIWLLSRRLDAVALGRDISTSLGVGHKRQVMITLTLVTVLMAMS
                     TSLVGPMTFLGFLVATLAYAVTDTYSHRRILPVAWLIGFVILGGAYFLLRHVLPMVDT
                     VTIIVELIGGLVFLIVVLRRGRL"
     gene            complement(3340..4386)
                     /locus_tag="VN041_17445"
     CDS             complement(3340..4386)
                     /locus_tag="VN041_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWU30868.1"
                     /translation="MASPGATTPRVTHVDALASRRTTRDDRHRSWVIRSLPLTIAIVL
                     TAGLLTLSVFTGVADITDTAGRGQEFAWITRIPRTVSLLLAGASMALAGLIMQLLTQN
                     RFVEPTTVGTTEWAGLGLLITYIALPMPSVMVRMIVAIVFAFAGTLIFFLFLRRVTLR
                     SSLIVPIVGIMFGAVVSAVSTFIALEFDLLQTLGAWFAGRFTGIEAGRYEPLWIVAVV
                     AVIVVLAADRFTVAGLGKDVATNAGLNYERVVLLGVGLVSIVTGVVTVVIGSLPFLGL
                     IVPNIVSMIRGDNLRTNIPWVFLTGIWIVTICDLIGRVVIMPFEVPVSLVLGAVGSVV
                     FILLLLRTRRTVAL"
     gene            complement(4462..5448)
                     /locus_tag="VN041_17450"
     CDS             complement(4462..5448)
                     /locus_tag="VN041_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWU30869.1"
                     /translation="MSVPRITSVVAIGAAALLALAGCSSAPTGQEAKEKPAAAAKAQY
                     PVTVTDMAGNEVTIESADSVVITDNRLFRLVADWGIDLSAAPRALMSPENPLKADESI
                     LDLGTHAEPDFEKVVEADPDLIVNGYRYGGHAEDMKKAAPDAAFIDMTNDELSTDQYL
                     VDSVELLGEVFGKQDEAKAVIDDFHAAVAAAKEAYDPDTTVMGLVTSGNEIRYSSPKD
                     GRGSSIFFDLVGLTPALQTDGSTNDKGDDISIEAIAQSDADFFLVLDREAAFADSESS
                     PALELINSSSALANVPAVQNKAIYVMPDDFYLTEDIVAYTTVLDGLTKAFSA"
     gene            5550..6314
                     /locus_tag="VN041_17455"
     CDS             5550..6314
                     /locus_tag="VN041_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HWU30870.1"
                     /translation="MTEVKSAFTIERRGLDLRFRALALTAREWLAPHYVRVRLSGPEL
                     EGFDSLGADDHMRLFFPEGPVPAEQLRASPSREYTPLAWGDDWLDVEFAVHGDEGVGA
                     PWAASAPLGSFVGVGGPRGSAVITGAPGSWLLAGDETAIPAIRRFAAAIPAGTPARIV
                     IEVATEADQVAIDAPVEVEWLHRSDAPAGSALITFLDGLETADAVSEDPFCFIAAEQS
                     VVKPGRALLTRWGVDPAQAIVKGYWKRGEAEYHAPH"
     gene            6340..6756
                     /locus_tag="VN041_17460"
     CDS             6340..6756
                     /locus_tag="VN041_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU30871.1"
                     /translation="MALLDHLGVSVLDIEQGRRQFHPVLTALGYTLGIEAPNGISWDN
                     GDETEIIIFPIRDEADAAPHRHGEAGWQHLAFSVASREEVDRLHAIAMDAGWTAVREP
                     KLYPRFNERYYASFVEDASGVRLEFVHNPPRDAAAG"
     gene            6846..7259
                     /locus_tag="VN041_17465"
     CDS             6846..7259
                     /locus_tag="VN041_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU30872.1"
                     /translation="MAVLTPYLSFRDNAREAMTFYQSVLGGKLDLVEFSDFADMPQDP
                     ADADKIMHSWLATEDGMVLAGSDTPTGMEYQPPQNISISISSDDEPRMQAIWDGLSDG
                     GTVTIPFETAPWGGRFGMLVDRFGMSWMLSVDEAQ"
     gene            complement(7263..7667)
                     /locus_tag="VN041_17470"
     CDS             complement(7263..7667)
                     /locus_tag="VN041_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HWU30873.1"
                     /translation="MTAVTLIRASALPDAAQYAYAATAPKEARLIFLAGACPLNDDGS
                     TAAVGDYPGQAAKCIETLEGALAAAGADLNDVISTRVLVASNRQRDLVTAWEVVRDAF
                     AAHDVPSTLLGVTVLGYDDQLVEIEAVAAVVD"
     gene            7730..8131
                     /locus_tag="VN041_17475"
     CDS             7730..8131
                     /locus_tag="VN041_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWU30874.1"
                     /translation="MATFDHLGISVDDVPAASAQFNPVFEALGYTRAYDAEEHAYWRR
                     TDEPEILLYKSREPGQHVHGRVGWQHLAFAVESREEVERVHAIALAAGWTEVRAPKEY
                     PRFTDRYYAAFIEDDNGIRLEIMHNPPRDAG"
     gene            8446..9615
                     /locus_tag="VN041_17480"
     CDS             8446..9615
                     /locus_tag="VN041_17480"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWU30875.1"
                     /translation="MERLSARLLSWASLIDEKTLDQAHTTARMPFIHPHLALMPDAHL
                     GKGATVGSVIPTLGAIIPAAVGVDIGCGMIAVRTQFTASEFAQRDLAPLREAIERAIP
                     LSAGRYNRKVVATAEPRIAELEGLAEKNGFDPASYAGNWMLQLGTLGSGNHFIEVSLD
                     ELDRVWLFLHSGSRGVGNKIATHHISVAQRLNRQWWIDLPDPDLAYLVEGTPEFTRYI
                     RELRWAQHFALLNREEMMDRVIRQVSEFAGTAVDEQERINCHHNFTESEKHFGKQVWV
                     SRKGAIQADAGRHGLIPGSMGTASYVVEGLGNPLSLNSSPHGAGREYSRSAARRTFTH
                     DQLRTAMAGIEFRDTDAFIDEIPQAYKPIDTVMADASDLVAIRHTLRQIVNVKGD"
     gene            complement(9734..11194)
                     /locus_tag="VN041_17485"
     CDS             complement(9734..11194)
                     /locus_tag="VN041_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30876.1"
                     /translation="MTRTASARATLGALAALVGFLAFVEFTSGVLQGYYTPMLTDIAR
                     HLGIHDADVNWLEGAQLMLSALVVPAFAKLGDMIGHKRMLLVSTALTAAAALVLPFTD
                     SFIVFLIGWALMGFYVVWLPLEIALIWSRSRSMEGRSVITARAAGMLVAALETGAILG
                     ALAGGALIDVLPLWVVLLIPALLIIVCFFVILFGVKESPELTGGRLDTVGIVLISLAL
                     IAFTGGLSLLRLPGGVSSFWSWGVVVLGILLVIPFVRWELRHDDPVIDVRMFRSPALG
                     PVFLTAGLFGVSVLGAQAPLSTFARTDPEVNGYGLGTTGFATSLIIGIYLIAMITGAL
                     LFPLIARLTAPRLTLMGASVLVGVGFLLFLPFHGTYLQVITNMVIVGLGSGALVAALP
                     AAAASAAPSTQTGVATGLTNSVKTVGGAIASCVFGIALLNGVVAGGAEGTAGSFSGYM
                     TVWIVCGATALAAAVLLAFVPKQAFTDRVAEDVPGV"
     gene            complement(11286..>11970)
                     /locus_tag="VN041_17490"
     CDS             complement(11286..>11970)
                     /locus_tag="VN041_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWU30877.1"
                     /translation="PPTLTAVRRIARAVDRLEPSMFPARTPKAISRMLAQFAARTPGA
                     AGFGLRALSRMPMITAQLFARLGGEPAALVRTTVAATMQSGGTAANVIPSQASATINL
                     RLALGETVDGTVLRVRDRIRDPKVAIEVLDGSDPSPESATDGAPWELLAGALAVSHPG
                     IPATPYVMMAATDSRHFHRFAPAVYRFAPLEMSSVQRATIHGVDEYVEIDALERGERF
                     HRALLERLG"
BASE COUNT         2008 a         4084 c         4137 g         1741 t
ORIGIN      
        1 aacgccgaga cccacgccac ggtcgccctc gctccgcagt ccgccggcgg ccgcgacctc
       61 gacgtcgtgc gtctcgccct gcacgagccc ggagcgatcg cccgtgccaa cgccgccgtc
      121 gaggaggaga aggacgagga ggccggcgtc gtcgtcgaca tctcccgcaa gcgcgtgctg
      181 ctggacggcg agtccgccgc cttcacctac aaggagttcg agctgctgca gtacctggtg
      241 ctgcgcgagg gccgcacgat cgagcgctcc gagctcgtct ccgccctgtg gcaggcgtcg
      301 accgacgagg acgcccccgg tgagcgcacg atcgatgtgc acgtccgccg cctgcgtgcc
      361 aagctcggcc gtcacgagga catcgtccgc accgtccgcg gcgtcggcta ccgcttcgac
      421 cgtcacgccg acgtcagcat ccgctacggc cacggcaccc cctcgcccga ccgcttctga
      481 ccgatcgcag ccacggcgca ggtgcgagaa ctgcagctgt cgcaggcagg cgatgtcagg
      541 cgtcgcgcgt agggtttcgg tatgaccctg acggatgccg cgccgaccgc gcacgcggtc
      601 ccgcgcgaat ccgtccatcg gcctcggcac ccgctggatc tgatggcgac ggtggggatg
      661 ctgcagcgcg gccgcaacga cccgaccatg gtcgtcgagg gcggccgcat ctggatggcg
      721 ttccgcacgg gtgccggtgt cgccacgctc tgcctccgtg cgacgtccgg cggcgtgcac
      781 gccacggcct ggggcccggg cgcggccgag gcgctcgatc gggtacctgc gctgtgcggt
      841 gctgatgacg atcagagcgg attcgacgtc tccgggcatc cgaggctcac ggaggtcgcg
      901 cggcgcaatc ccggcatccg cctgacccgc accggccggg tcttcgatgc gctcacctgc
      961 gcgatcatcg agcagaaggt caccggcatg caggcgttcg gcgcgtggcg gcatctggtc
     1021 agccggaacg gcgagcgtgc tcccgggccg acgccgcggc ccatgttcgc ggcaccgcac
     1081 tcggaggtgt ggcgggcgat cccatcgtgg gggttccatc gcgcagggct cgagccgcct
     1141 caggcgcgca cgatagcccg cgcggcgatg cgcggtccga gcatcgagcg cgccgtgctc
     1201 gcggcatccg acggcgacgg acgtgagcgc gtgctgacca gcctgcctgg catcgggccg
     1261 tggacttcgg ccgagacccg catccgcgcg ctcggtgacc ccgacgccgt gagcgtcggg
     1321 gacttccatc tcgcgcacga ggtcggcttc gcgctgaccg gcagccggac cgatgacgac
     1381 gggatgctgg agctgctggc cccttgggca gggcaccggc agcgcatcat ccggctgatc
     1441 ttcgcctccg gcgtccgcga gccgcgccgt ggcgcacgcc tcgctcccga ggatcatcgc
     1501 agccgctgag tcggcggaga ctcatcggtg gtacacggcg aacttcccgc cgggcccgtc
     1561 gacgacggtc acgggggtgt ggaacaccgc ggtcagcacc tcgtcgcgga tgatctcctc
     1621 gggagtgccg tgcacggcga tacgcccgct ctccatcgcg acgatgtagt cgctgtacgc
     1681 ggccgcgaag ttgatgtcat gcagcacgac cacgatcgtg cgcccgagcc gatctgcggc
     1741 ccggcgcagc tggcgcatca tctgcacgga atgctgtgcg tcgaggttgt tcagcggctc
     1801 gtccagcagc agatactcgg tgtcctgcgc gagcaccatc gcgacgtagg cgcgctgtcg
     1861 ctgccctccg gagagctggt cgaggaagcg ctgctccagg tccgtgagat tcaggaagtc
     1921 gatcgcctca gagatcttgt cctcgtcgcg ctgggtgagc cggccgccgc tgtaggggta
     1981 gcgcccgaac ccgaccagct gccgcaccgt gagcctggtg acgaagtggt tctcctgtcg
     2041 caggatcgac acggtcctgg cgacctgctt cgacggcatc cggtgcacgt ccaggccgcc
     2101 gacctccacg gttcccgagt ccggtgccag gagccggccc atcatggtga gcaccgtcga
     2161 cttgcccgcg ccgttgggtc ccaccagggc gatgatgccg ccggcgggca gatcgatcga
     2221 gatcgggccg atctcgacgt cgccgctgta gcgcttgatg acgttggaca gacggatcac
     2281 aggcgtcccc ttctcagaac gacgatgagg aagacgagcc cgccgatgag ctcgacgatg
     2341 atggtgaccg tgtcgaccat cggcagcacg tgcctcagca ggaagtaggc gccgccgagg
     2401 atgacgaagc cgatcagcca cgccaccggc aggatgcggc gatggctgta ggtgtcggtg
     2461 accgcgtagg ccagggtggc gacgaggaag ccgaggaagg tcatgggccc caccagggac
     2521 gtgctcatcg ccatcagcac ggtcaccagg gtgagcgtga tcatgacctg cctcttgtga
     2581 cccaccccca ggctggtgct gatgtctcgg ccgagcgcca cggcgtcgag gcgtctcgag
     2641 agcagccaga tcccgcccgc ggctcccacg cacaccggga tcacgaacca caggtactcg
     2701 gtgttcgcgt tgccgacgtt gccgaacata cgggcgcgca gcacatcgaa ctcgttagga
     2761 tccagcatcc gctgcatgaa cgccgacagc gccccgaggc cggtgccgat gatgatcccc
     2821 accaggagca tgacatgaag gttgccgagc cgccccgaga acagccagga gtagagcagc
     2881 acggcgaaga ccaccatccc cgcggcctgg atcacgaact gcgcgaaccc gtcgatctcg
     2941 gccaggccgc tgacgccgaa gaagaacacg accgccgtct ggatgaggat gtacagcgac
     3001 tcgaacccca tgatcgacgg agagaggatg cgattgccgg tgacggtctg gaaggacacg
     3061 gtcgcgaagg actggcacag cgtcacgagc gcgatcacga tgagggattc ggatcgcatc
     3121 cgcgagaccc gccaccaccg ctccgtgaac gggatctgac tgttgcccca ggtcagcacc
     3181 agcacgacca gcgcgagcga ggcgacgatg aggcccgcga ggatcagcag gtagcggcgg
     3241 cgatgcgcac gcccggagaa cgggccgaca cgacggtcgg acgagggagc ggaggctccg
     3301 agaggcgtcg ccgacatggg agcgggcgtg aacgagacgt cagagcgcaa cggtcctcct
     3361 tgtgcgcagc agcaagagga tgaacacgac ggagcccacc gccccgagga ccagggagac
     3421 cggcacctcg aagggcatga tcaccacgcg gccgatcagg tcgcagatcg tgacgatcca
     3481 gatgccggtc aggaacaccc aggggatgtt ggtgcggaga ttgtccccgc ggatcatcga
     3541 gacgatgttc ggcacgatca gccccaggaa cggaagcgaa ccgatcacga ccgtgaccac
     3601 gcccgtgacg atcgagacga gcccgacccc cagcagcacg acgcgttcgt agttcagtcc
     3661 ggcgttggtg gcgacatcct tgccgagccc cgcgaccgtg aagcgatcgg cggccagcac
     3721 cacgatgacc gcgacgaccg ccacgatcca caacggctcg taacggccgg cctcgatgcc
     3781 ggtgaaccgg ccggcgaacc aggcgccgag cgtctgcagc agatcgaact ccaaggcgat
     3841 gaaggtggag accgcgctca cgaccgcacc gaacatgatg ccgacgatgg gcacgatgag
     3901 ggacgagcgc agcgttaccc ggcgcaggaa gaggaagaag atcagggtcc ccgcgaaggc
     3961 gaacacgatc gcgacgatca tgcgcaccat gaccgagggc atgggcagcg cgatgtaggt
     4021 gatcagcagc ccgagacccg cccactcggt cgtgccgacg gtggtgggct cgacgaaccg
     4081 gttctgcgtc aggagctgca tgatcaggcc cgccagcgcc atgctcgctc ccgcgagcag
     4141 cagcgagacc gtgcgcggga tgcgggtgat ccaggcgaac tcttggccgc ggcccgcggt
     4201 gtcggtgatg tcggcgacgc cggtgaacac ggagagggtc agcaggcccg ccgtcagcac
     4261 gatcgcgatc gtgagcggca ggctcctgat cacccacgac cgatgtcggt catcgcgggt
     4321 ggttcttcgg gaggcgagcg cgtccacgtg cgtcacgcgc ggtgtcgtcg cgccgggcga
     4381 agccatgaag gggtcctttg tcatagggca cgtgcatggc cgcgatccgg tggaacgcgg
     4441 ccatgcatgg ctgtggccgc gttacgcgga gaacgccttc gtcaggccgt cgagcacggt
     4501 ggtgtacgcg acgatgtcct cggtgaggta gaagtcgtcg ggcatcacgt agatggcctt
     4561 gttctgcact gcggggacgt tcgcgagggc gctggagctg ttgatgagct cgagggccgg
     4621 cgacgactcg ctgtccgcga aggcggcctc gcggtcgagc acgaggaaga agtcggcgtc
     4681 ggactgggcg atggcctcga tcgagatgtc atcgcccttg tcgttcgtcg acccgtcagt
     4741 ctgcagtgcg ggagtcagac ccaccaggtc gaagaagatg ctcgaccctc gaccgtcctt
     4801 cgggctcgag tagcggatct cgttgccgct ggtcaccagg cccatgacag tcgtgtccgg
     4861 gtcgtatgcc tccttcgccg ccgcgacggc cgcgtggaag tcgtcgatca ccgccttcgc
     4921 ctcgtcctgc ttgccgaaca cctcgcccag cagctcgacg ctgtccacca ggtactggtc
     4981 ggtgctgagc tcgtcgttgg tcatgtcgat gaaggcggcg tcgggtgcgg ccttcttcat
     5041 gtcctcggcg tgcccgccgt agcggtagcc gttcacgatc aggtcggggt cggcctcgac
     5101 gaccttctcg aagtcgggct ccgcgtgggt gccgagatcg aggatcgact catccgcctt
     5161 gagcgggttc tcggggctca tcagagcgcg aggcgctgcg gacaggtcga tgccccagtc
     5221 cgccacgagc cggaagagac ggttgtccgt gatgacgaca ctgtccgcgc tctcgatcgt
     5281 gacctcgttc cccgccatgt ccgtcacagt caccggatac tgggccttgg ccgccgcggc
     5341 aggcttctcc ttggcttcct gtccggtcgg cgcgctgctg cagccggcga gagcgaggag
     5401 tgcggcggcg ccgatggcga cgaccgaggt gattctgggc acggacatgg agagtctcct
     5461 gttctgcttc tgagaggtgc gcgaaggatg cgcggacgcc ggaatttagg taacccttac
     5521 ccggctccga attaggttag ccttacttta tgaccgaagt gaaatccgcc ttcacgatcg
     5581 aacgccgcgg cctcgatctg cgcttccgcg ccctcgccct gaccgcccgc gagtggctcg
     5641 cgccgcatta cgtgcgcgtg cgcctgagcg gtccggagct cgagggcttc gactccctcg
     5701 gcgccgacga ccacatgcgg ctgttctttc ccgagggccc cgtgccggcg gagcagctgc
     5761 gcgcgtcgcc cagccgtgag tacacgccgc tggcctgggg cgatgactgg ctcgacgtgg
     5821 agttcgccgt gcacggcgac gagggcgtgg gcgcgccgtg ggcggcatcc gctcccctcg
     5881 gctcgttcgt gggcgtcggc ggtccgcgtg gatccgccgt aatcaccggc gccccgggct
     5941 cctggctgct cgccggagac gagacggcca tccccgcgat ccgccgcttc gccgcggcca
     6001 tccccgccgg cactccggcg cgaatcgtca tcgaggtcgc gacggaggcc gatcaggtgg
     6061 caatcgatgc gccggtcgag gtggagtggc tgcaccgcag cgacgctcca gccggctccg
     6121 cgctgatcac gttcctggac gggctcgaga cggcggatgc cgtcagcgag gacccgttct
     6181 gcttcatcgc cgccgagcag tcggtcgtga agcccggtcg cgcgctgctg acccgctggg
     6241 gtgtggaccc ggcgcaggcg atcgtgaagg gctactggaa gcgcggcgag gccgagtacc
     6301 acgcaccgca ctgacggacg cgtcggccag gatggtcgca tggctcttct ggatcacctc
     6361 ggcgtctcag tgctcgacat cgaacagggc agacggcagt tccacccggt gctgacggcg
     6421 ctcggctaca ccctcggcat cgaggcgccg aacggcatct cgtgggacaa cggcgacgag
     6481 accgagatca tcatcttccc catccgggac gaagcggatg ccgcaccgca ccgccacggc
     6541 gaggccggat ggcagcacct cgcgttctcc gtcgcgtccc gcgaggaggt cgaccgcctg
     6601 cacgcgatcg cgatggacgc ggggtggacc gcggtgcgcg agccgaagct ctacccgcgg
     6661 ttcaacgagc gctactacgc gtcgttcgtg gaggacgcga gcggcgtccg tctggagttc
     6721 gtgcacaacc cgccgcggga cgccgcagca gggtgagaac cccttgccga tgtcggtggc
     6781 cggtcgcaga ctgatctgcg tcgggcgatc acgctcgacg atccgcctct ccggaaggtg
     6841 aaccgatggc tgtcctgacc ccctacctct cgttccgcga caacgcgcgc gaggcgatga
     6901 cgttctatca atcggtgctc ggcggcaagc tcgacctggt cgagttctcg gacttcgccg
     6961 acatgccgca ggatcccgcc gacgcggaca agatcatgca ctcgtggctc gccaccgagg
     7021 acggcatggt gctcgccggc tccgacaccc ccaccggcat ggagtaccag ccgccgcaga
     7081 acatctcgat ctcgatcagc agcgatgacg agccgcggat gcaggcgatc tgggacggcc
     7141 tgagcgacgg cggcaccgtg acgataccgt tcgagaccgc tccgtggggc gggcgcttcg
     7201 gcatgctcgt cgatcgcttc ggcatgtcct ggatgctctc cgtcgacgag gcgcagtagc
     7261 actcagtcca cgacggcggc gacggcctcg atctcgacga gctggtcgtc atagccgagc
     7321 accgtcaccc ccaggagcgt gctcgggacg tcgtgcgcgg cgaacgcgtc gcgcacgacc
     7381 tcccaagccg tgacgagatc gcgctgtcgg ttcgatgcga cgagcacccg ggtgctgatc
     7441 acgtcgttca ggtctgcgcc ggcggcggcg agcgcaccct ccagcgtctc gatgcacttc
     7501 gcggcctgac ccggatagtc gccgacggcc gcggtcgagc cgtcgtcgtt caacgggcag
     7561 gcgccggcga ggaagatcag gcgcgcctcc ttcggagcag tggcggcgta ggcgtactgg
     7621 gcggcgtcgg gcagggcgga ggcccggatg agggtcacag cggtcatggg atcagtctgc
     7681 cgcgccgctg gatcgagcgg atgtcggcgg catccggcac actgtgggga tggcgacctt
     7741 cgatcacctc ggcatcagcg tcgatgacgt tcccgcggca tccgctcagt tcaatccggt
     7801 cttcgaggcc ctcggataca cccgcgcgta cgacgcggag gagcacgcgt actggcgacg
     7861 caccgacgag cccgagatcc tgctctacaa gtcgcgcgag cccggccagc acgtgcacgg
     7921 ccgggtcggc tggcagcacc tcgcgttcgc cgtcgagtcc cgcgaagagg tcgagcgcgt
     7981 ccacgcgatc gccctggccg cgggctggac cgaggtgagg gcacccaagg agtacccgcg
     8041 gttcaccgac cgctactacg cggccttcat cgaggatgac aacggcatcc ggctcgagat
     8101 catgcacaac ccgccgcgcg acgcgggctg atcaccctgc cgggccgcac cccagctcct
     8161 tcgagaactc gcccttgacc tgccgcgcga tcctccccta tggttgcaaa gctgcgccga
     8221 ccggtgcagc agccagccga ggagacgacg gatgaccgcc ctgcgcgccc ttcccacggt
     8281 ccttgccttc gagcagaagc gcaacgaccg cgacgagtcg taccctccgg cgcgcgccgt
     8341 gtgacagcat ccgctgctcg catgccccgc accgtcaggt ccggggcatt tttcatgaat
     8401 cctccgtgac ctgtgaccgg cacacatcac agaaggaaag gaatcatgga gaggctctcc
     8461 gcacggctgc tgtcgtgggc gtcgctgatc gacgagaaga cgctcgatca ggcgcacacc
     8521 acggcgcgca tgccgttcat ccacccgcac ctggcgctca tgccggatgc gcacctcggc
     8581 aagggggcga cggtcggctc ggtcatcccg acgctcggcg cgatcatccc cgccgccgtc
     8641 ggtgtcgaca tcggctgcgg gatgatcgcc gtccgcacgc agttcacggc atccgagttc
     8701 gcccagcgcg acctcgcgcc gctgcgggag gcgatcgagc gcgccatccc gctctcggca
     8761 ggccggtaca accgcaaggt cgtcgccacc gccgagccga ggatcgcgga gctggagggc
     8821 ctcgccgaga agaacggctt cgaccccgcg tcgtacgccg gaaactggat gctgcagctg
     8881 ggcacgctcg gctcgggcaa ccacttcatc gaggtgtcgc tcgacgagct cgaccgggtc
     8941 tggctgttcc tgcactccgg ctcccggggc gtgggcaaca agatcgccac gcaccacatc
     9001 tcggtcgcgc agcggctgaa caggcagtgg tggatcgacc ttcccgaccc cgacctggcg
     9061 tacctggtcg agggcacccc cgagttcacc cggtacatcc gggagctgcg gtgggcccag
     9121 cacttcgccc tgctgaaccg ggaggagatg atggacaggg tgatccggca ggtctcggag
     9181 ttcgccggca ctgcggtgga cgagcaggag cggatcaact gccatcacaa cttcaccgag
     9241 tcggagaagc acttcggcaa gcaggtgtgg gtgtcgcgga agggcgcgat ccaggcggac
     9301 gcaggtcggc acgggctcat ccccggttcg atgggcaccg cctcgtacgt ggtggaggga
     9361 ctcggcaacc cgctgtcgct gaactcctca ccgcacggcg cgggccggga gtactcccgg
     9421 tcggcggcgc ggcgcacctt cacacacgat cagctgcgga cggcgatggc gggcatcgag
     9481 ttccgtgaca cggacgcctt catcgacgag atcccgcagg cgtacaagcc gatcgacacc
     9541 gtgatggcgg acgcatccga cctcgtggcg atccggcaca cgctgcgcca gatcgtcaac
     9601 gtgaagggcg actgaccggc atccgctcgg gcactgcctg gtacggatgc acgacccggg
     9661 aacgaacgca cgatggatca cgcgattcag tcgtgcaggt gttcccgggt cgtgcgggtg
     9721 ttcccgagcg cggtcagacg cccgggacat cctccgcgac gcgatcggtg aacgcctgct
     9781 tcggcacgaa cgccagcagc acagcggcgg cgagcgcggt cgcaccgcac acgatccaca
     9841 ccgtcatgta acccgagaag gatcccgccg tgccctccgc gccgccggcg accacgccgt
     9901 tcagcagcgc gatgccgaac acgcacgacg cgatcgcccc gcccacggtc ttcaccgagt
     9961 tcgtcaggcc ggtcgcgacg cccgtctggg tggagggggc agcggatgcc gcggcggccg
    10021 gcagcgcagc caccagcgca cccgatccga ggccgacgat caccatgttg gtgatcacct
    10081 gcagatatgt gccgtggaac ggcaggaaca gcaggaagcc gacgccgacc agcaccgagg
    10141 cgcccatcag cgtgagccgc ggcgcggtca gccgcgcgat cagcgggaac agcagtgcgc
    10201 cggtgatcat cgcgatcagg tagatgccga tgatcagcga ggtcgcgaat cctgtcgtgc
    10261 cgaggccgta gccgttcacc tccgggtcgg tgcgggcgaa ggtcgacagg ggtgcctgtg
    10321 cgccgagcac gctcacgccg aacagcccgg cggtgaggaa caccggaccg agcgcgggcg
    10381 agcggaacat gcgcacgtcg atcaccggat cgtcgtgacg cagctcccag cgcacgaacg
    10441 ggatcaccag caggatgccg agcaccacca cgccccacga ccagaagctc gacacgccgc
    10501 ccggcagccg cagcaggctg aggcctcccg tgaaggcgat gagcgccagc gagatgagca
    10561 cgatgccgac ggtgtccagc cgtccgccgg tcagctccgg cgattccttc accccgaaca
    10621 ggatgacgaa gaagcagacg atgatcagca gggcggggat gagcagcacc acccacagcg
    10681 gcaggacgtc gatcagcgcc ccgccggcga gcgcaccgag gatcgcaccg gtctccaggg
    10741 ccgcgacgag catgcccgcc gcccgtgccg tgatcaccga gcggccctcc atcgagcgcg
    10801 accgcgacca gatcagcgcg atctccaacg gcagccacac cacgtagaac cccatcagcg
    10861 cccagccgat caggaagacg atgaaggagt cggtgaacgg cagcaccagc gctgccgccg
    10921 cggtgagcgc ggtcgagacc agcagcatcc gcttgtgccc gatcatgtcg ccgagcttcg
    10981 cgaacgccgg cacgacgagc gccgagagca tcagctgggc gccctcgagc cagttcacgt
    11041 ccgcgtcgtg gatgccgagg tgccgggcga tgtccgtgag catgggcgtg tagtacccct
    11101 gcagcacgcc gctggtgaac tcgacgaagg cgaggaagcc gaccaaggcg gcgagtgcgc
    11161 ccagggtcgc gcgggcggat gcggtgcgcg tcatcgcgcc tcctgttcgg gttcagggac
    11221 gacccttcga caggctcagg gacccgggaa agaggcagca gggaccggtc gcagggcggg
    11281 tgagatcacc ctagccgttc gaggagcgcg cgatggaacc gctcaccgcg ctccagcgcg
    11341 tcgatctcga cgtactcgtc caccccgtgg atggtggctc gctgcacgga tgacatctcc
    11401 agcggcgcga atcggtagac ggcgggcgcg aagcggtgga agtgccggga gtcggtcgcc
    11461 gccatcatca cgtacggcgt cgccgggatg ccgggatgcg acaccgccag cgcaccggcg
    11521 agcagctccc acggggcgcc atcggtcgcg gactccgggg aggggtcgct gccgtcgagc
    11581 acttcgatcg ccaccttcgg atcgcggatg cggtcacgca cccgcagcac ggtgccgtcc
    11641 acggtctcgc cgagagcgag gcgcaggttg atcgtggcgg atgcctgcga ggggatgacg
    11701 ttcgccgcgg ttccgccgga ttgcatggtc gcggcgaccg tggtgcgcac cagcgccgcg
    11761 ggttcgccgc cgaggcgggc gaacagctgc gccgtgatca tcggcatccg gctcagcgcc
    11821 ctcagtccga agccggccgc acccggtgtg cgagcggcga actgcgccag catccgactg
    11881 atcgccttcg gcgtgcgcgc ggggaacatc gatggttcca ggcggtcgac ggcgcgggcg
    11941 atgcggcgta ctgccgtgag cgtcggcggg
//
LOCUS       DATMSX010000552         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_179218,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000552 DATMSX010000000
VERSION     DATMSX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179218"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN041_17495"
     CDS             <1..185
                     /locus_tag="VN041_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWU30878.1"
                     /translation="SATVVAESLTRADVWATAIVAAGADLDWLPRATGTSGLLVDAAG
                     RIRRWIEGVTVEVQPA"
     gene            complement(386..>1732)
                     /locus_tag="VN041_17500"
     CDS             complement(386..>1732)
                     /locus_tag="VN041_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HWU30879.1"
                     /translation="PEPTRVRRPRRERVPGSRLRTLAILGGADGPILDRVPGETPRFV
                     QMFFVLAGTALVSAISMLFALTTGVQVAIWLAVPLAIVWAAIIFNLDRFLTATMSSSR
                     SIGKLIALAVPRVAMAALIGFLVAEPLVLQVFHNDIAREVASTNISQSQSDQKALEKG
                     PEKKALDAATKRVAALENQAATGIVAGTDKGSASTAAAKSTVDDLTGKMAEQQKVIDS
                     ARALYQCELTGEGAGTVPGCTGVHGQGSSSDAAKAQLAQAQQTYDALAAQLRAANGDL
                     SSAETAEKANTTASESTNRQQAKDQLPAAKKTYENALAAYNARADSVAQGNAGAIGLL
                     SQITGLNRLADKEPAIFWAHWLIAALFFMIELLPVLVKVLTSYGDPSLYEKTAAIRKQ
                     VELDRVTADGFRDRAAIVAEDAPAPTASAPPRLPDDHDGTPTVPLTRADIRESVSV"
BASE COUNT          238 a          588 c          635 g          271 t
ORIGIN      
        1 gctcggccac ggtcgtcgcc gagagcctca cccgtgcgga tgtctgggcc accgcgatcg
       61 tcgccgcggg cgccgatctg gactggcttc cccgcgcgac gggcacatca ggcctgctcg
      121 tcgacgccgc cggccgcatc cgccgctgga tcgagggcgt gaccgtcgag gtgcagcccg
      181 cctgagctga tgcttgtcga gtgacctcgg ccctccatgt cgcagtggat gccgctaccg
      241 cccgcgcgcc cttcgctcag gtcgcaatag atgccgtcgg gatcgcgaat cggcggcatc
      301 catcgcgacc tggagagcga ccgtctgcag aacggcccgc gtggcgcagg gtggccgacg
      361 cataatcgtg acctgggcgg cgaagtcaga cggacacgga ctcgcggatg tcggcgcggg
      421 tcagcggcac ggtcggggtg ccgtcgtgat cgtccggcaa ccgtggcggt gcggatgccg
      481 tcggagccgg ggcatcctcc gcgacgatcg ccgcgcggtc gcggaagccg tcggcggtca
      541 cccggtcgag ctcgacctgc ttccggatcg cggcggtctt ctcgtacagc gacgggtcgc
      601 cgtaactggt gagcaccttc accaggaccg gcagcagctc gatcatgaag aagagtgcag
      661 cgatcagcca gtgggcccag aagatcgcgg gctccttgtc ggccaggcgg ttcaggccgg
      721 tgatctggct gagcagcccg atcgcccccg cgttgccctg cgcaaccgaa tccgcccgcg
      781 cgttgtacgc ggcgagcgcg ttctcgtagg tcttcttcgc cgcgggcagc tggtccttgg
      841 cctgctggcg gttggtcgac tcggatgccg tggtgttggc cttctcggcc gtctcggcgc
      901 tggacaggtc gccgttggcg gcgcgcagct gcgcggccag agcgtcgtag gtctgctggg
      961 cctgggcgag ctgggccttc gcggcatccg agctggaacc ctgaccgtgc acgccggtgc
     1021 acccgggcac cgtgcccgcg ccctccccgg tcagctcgca ctggtacagc gcacgggcgg
     1081 agtcgatcac cttctgctgc tcggccatct tgccggtgag atcgtcgacc gtggacttgg
     1141 cggcggcggt cgaggccgag cccttgtcgg tgccggcgac gatgccggtc gcggcctggt
     1201 tctcgagcgc ggcgacccgc ttggtcgcgg cgtcgagggc cttcttctcc ggccccttct
     1261 cgagggcctt ctggtcggac tgcgactggg agatgttggt ggacgccacc tcgcgggcga
     1321 tgtcgttgtg gaacacctgc agcacgagcg gctccgcgac gaggaagccg atcagcgccg
     1381 ccatcgccac gcgcgggacg gccagcgcga tcagtttgcc gatgctgcgc gacgacgaca
     1441 tggtcgccgt gaggaagcgg tcgaggttga agatgatcgc cgcccacacg atcgccagcg
     1501 gcacggccag ccagatcgcc acctgcacgc cggtcgtgag cgcgaacagc atcgagatcg
     1561 cagagaccag cgcggtaccg gcgagcacga agaacatctg cacgaagcgg ggcgtctcgc
     1621 ccggaacgcg gtcgaggatc gggccgtcgg caccgccgag gatcgcgagc gtgcgcaggc
     1681 gggaacccgg tactcgctcg cggcggggac gccggacgcg ggtgggctcc gg
//
LOCUS       DATMSX010000553         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_188367,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000553 DATMSX010000000
VERSION     DATMSX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188367"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VN041_17505"
     CDS             <1..748
                     /locus_tag="VN041_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWU30880.1"
                     /translation="SHAATLIADGLVRVAGHPVVKASTPVTDDTEIRVDGADHYVSRG
                     AHKLIAALDGFALDLQGRLALDMGASTGGFTQVLRERGARRVLAVDVGHGQLAPSIAA
                     DAGVTSVEGFNVRHMTAESLADATGEPARPELVVGDLSFISLEHVLPAVAGVIAPGGD
                     VVLLVKPQFEVGRTAIKGGLVTNPALRVDAVERTLWTAHDSGLGMLGILASPILGTHG
                     NAEYLVHLAPDRGTDPSEWSEQIADVAGGR"
     gene            745..1650
                     /locus_tag="VN041_17510"
     CDS             745..1650
                     /locus_tag="VN041_17510"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010202091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HWU30881.1"
                     /translation="MNERRILVVAHAKRPDTVDAALRVIDALHQAGAVPVLAAPDREE
                     LSDVDARFAGIAQLGEDVAAEDLELAIVLGGDGTILRAAELVRDSGAPVLGINMGHVG
                     FLAEIDRDDMDAAVHRVIARDYEVEERMALSVRVKDVEGTVVYETWALNEATVEKASR
                     ERMIEVVIEIDGLPLSSFGCDGMVVSTPTGSTAYNFSAGGPVIWPTVEAIAVVPLSAH
                     ALFAKPLVVGPEASVAIEMLERTSGTGILWCDGRRSHDLPPGARVIVRRSSRPVRLAR
                     LHPTAFTNRLVRKFQLPVAGWRGAS"
     gene            1647..>2711
                     /locus_tag="VN041_17515"
     CDS             1647..>2711
                     /locus_tag="VN041_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU30882.1"
                     /translation="MIDEMRIRGLGVIDDAVLPLGPGFTAVTGETGAGKTMVVTGLGL
                     LLGARADSSAVRAGASQASVAGVWLVPPHGPIADIVTDAGGELEPAGDASELYVSRTL
                     SAEGRSRASVGGRAAPASVLSALADELVVVHGQSDQLRLRSASAQRDALDRFGGERIA
                     TALSEYTERFQAWRALDAEIAELTENRDRRAAEAAQLREQLAEIEQLEPEAGEDVSLN
                     ERAERLANAEELRLSASQARTALSSEDDEPDVAGLIAEARRSLERSADPQLTEIAETL
                     ADLGYRAADLAGQLSTYLADLDESGPHELAAVEERRAAIGGLIRQHGSLEQALEVWRT
                     GSLRLAELDDDGDRIERLAAQ"
BASE COUNT          390 a          991 c          961 g          369 t
ORIGIN      
        1 ctcgcacgcc gcgaccctga tcgccgacgg gctcgtccgg gtggccggac accccgtcgt
       61 caaggcttcg acgccggtca ccgacgacac cgagatccgc gtcgacggcg ccgaccacta
      121 cgtcagccgc ggtgcccaca agctcatcgc cgccctcgac ggcttcgcgc tcgacctcca
      181 gggcagactc gccctcgaca tgggagcctc caccggcgga ttcacgcagg tgctgcgcga
      241 gcgcggcgcg cgccgcgtgc tggcggtgga cgtcggccac ggtcagctcg ccccgtccat
      301 cgccgcggat gccggcgtca cctcggtcga gggattcaac gtgcggcaca tgaccgcgga
      361 gagcctcgcg gacgccacgg gcgagcccgc ccgacccgag ctcgtggtcg gcgacctctc
      421 cttcatctca ctggagcacg tgctgccggc cgtcgccgga gtgatcgcgc ccggcggtga
      481 cgtcgtgctg ctggtgaagc cccagttcga ggtgggccgc acggccatca agggcgggct
      541 cgtcaccaac cccgcactgc gggtggacgc ggtcgagcgc accctgtgga ccgcgcacga
      601 cagcgggctg ggcatgctcg gcatcctggc ctcgcccatc ctcggcacgc acggcaacgc
      661 cgaatacctg gtgcacctcg cgcccgaccg cggcacggat ccgtcagaat ggtcggagca
      721 gatcgcagac gtggcaggag gacgatgaac gagcgccgga tcctggtcgt cgcccacgcg
      781 aagcggcccg acacggtcga tgcggccctg cgcgtgatcg acgcactgca ccaggcgggt
      841 gcggttcccg tgctcgcggc gcccgatcgt gaggagctct ccgacgtcga cgcccgtttc
      901 gccgggatcg cccagctggg cgaggacgtg gcggccgagg acctcgagct ggcgatcgtc
      961 ctcggcggcg acggaacgat cctgcgcgcg gccgagctgg tccgcgactc cggagcgccc
     1021 gtgctcggca tcaacatggg acacgtcggc ttcctggccg agatcgaccg cgacgacatg
     1081 gatgccgccg tgcaccgggt catcgcgcgc gattacgagg tcgaggagcg catggcgctg
     1141 tcggtgcggg tgaaggacgt cgagggcacc gtcgtctacg agacgtgggc cctgaacgag
     1201 gcgaccgtcg agaaggccag ccgcgagcgc atgatcgagg tcgtcatcga gatcgacggc
     1261 ctgcccctat cgagcttcgg atgcgacggg atggtggtct ccacgccgac aggctccacc
     1321 gcctacaact tctccgccgg cgggcccgtg atctggccga ccgtggaggc catcgccgtc
     1381 gtgccgctgt ccgcgcacgc gctgttcgcg aagccgctcg tcgtcgggcc agaggcgtcc
     1441 gtcgcgatcg agatgctcga gcgcacgagc ggcaccggca tcctgtggtg cgacggcaga
     1501 cgctcccacg acctgccgcc cggagcgcgc gtcatcgtgc gccggtcgtc acggccagtg
     1561 cggctcgcgc gtctgcatcc gacggccttc acgaaccgac tggtccgcaa gttccagctg
     1621 cccgtcgccg gatggcgagg tgcctcgtga tcgatgagat gcgcatccgc ggcctgggcg
     1681 tgatcgacga cgccgtgctg ccgctgggtc ccgggttcac cgccgtcacc ggcgagaccg
     1741 gagcgggcaa gaccatggtc gtcaccggac tcggcctgct gctcggggcc agggcggatt
     1801 cgtccgccgt gcgggccgga gcctcgcagg cgtccgtcgc gggcgtctgg ctggtgccgc
     1861 cgcacgggcc gatcgccgac atcgtcacgg atgccggcgg cgagctcgaa cccgcaggcg
     1921 atgcctccga gctgtacgtc tcgcgcacgc tcagcgccga ggggcgcagt cgcgcgagtg
     1981 tgggcggccg tgccgccccc gcgtccgtgc tctccgccct cgccgatgag ctggtggtcg
     2041 tgcacggcca atccgatcag ctgcgcctgc ggtcggcatc cgctcagcgc gacgccctcg
     2101 accgcttcgg cggcgagcgg atcgccaccg cgctgtccga gtacaccgag cgtttccagg
     2161 cctggcgtgc actggatgct gagatcgccg agctcaccga gaaccgtgac cgtcgcgccg
     2221 ccgaggccgc gcagctgcgc gagcagctcg ccgagatcga gcagctcgaa cccgaagcgg
     2281 gggaggacgt ctcactgaac gagcgcgccg agcgcctcgc gaacgccgag gagctgcggc
     2341 tgtcggcgtc ccaggcacgc accgcgctct ccagcgagga cgacgagccc gacgtggcag
     2401 gactcatcgc cgaggcacgg cgttcactgg agcgctccgc ggacccgcag ctcaccgaga
     2461 tcgcggagac cctggccgac ctcggctacc gcgccgcaga cctcgcgggg cagctgtcca
     2521 cctatctcgc cgacctcgac gagtcgggtc cgcacgagct ggccgccgtc gaggagcgcc
     2581 gtgcggcgat cggcggactg atcagacagc acggctccct ggagcaggcg ctcgaagtgt
     2641 ggcggacggg ctccctgcgc ctggccgagc tcgacgacga cggcgaccgc atcgagcggc
     2701 tcgccgcaca g
//
LOCUS       DATMSX010000554         5389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_9094,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000554 DATMSX010000000
VERSION     DATMSX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9094"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..109)
                     /locus_tag="VN041_17520"
     tRNA            complement(37..109)
                     /locus_tag="VN041_17520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Thr,seq:tgt)
     gene            complement(165..1133)
                     /locus_tag="VN041_17525"
     CDS             complement(165..1133)
                     /locus_tag="VN041_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWU30883.1"
                     /translation="MSSDETPNVPAARNSREAVREKAQRVHAQQSRARVMRRVIIGLV
                     AAVAVGSIGTAVAFAVSSSMSKPMLSPSGMTDDGVTVSDVTLASGGADISPNASPTPS
                     EAAATPTPKTTEKAAEPTQIHIYVDYLSAGAAEFEKANARQLAGWIKQGAVTVTYHPV
                     ALLMASSNGTKYSLRAAAAAACVATYSPDQFFDYNHELLTDQPKIDSDGRSDVELADL
                     AVAVGVQNAKKVRSCIEGQDFVSWAKDATARALEGPLPGSKDLKLNNEALIVVGGQAY
                     VGALNNPAEFSQFVLTTASDEYYGETDPTPTPTPPASPAPTETATP"
     gene            1349..2452
                     /gene="ugpC"
                     /locus_tag="VN041_17530"
     CDS             1349..2452
                     /gene="ugpC"
                     /locus_tag="VN041_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWU30884.1"
                     /translation="MATVTFDEATRIYPGGTRPAVDKLNIDIADGEFLVLVGPSGCGK
                     STSLRMLAGLEEVNAGRILIGDRDVTDVPPKDRDIAMVFQNYALYPHMTVAENMGFAL
                     KIAGVGKEERAQRVLEAAKLLDLEEYLNRKPKALSGGQRQRVAMGRAIVRQPQVFLMD
                     EPLSNLDAKLRVQTRTQIASLQRRLGVTTVYVTHDQTEALTMGDRIAVLKDGLLQQVG
                     SPRDLYEKPNNVFVAGFIGSPAMNLFPADLAEGGVRFGTEVVPLDRDTVGRANGSQVM
                     VGVRPEDITVGPADGKGISVVVDLVEELGADGYLYGHADINGKRADIVARVDGRSHPS
                     AGETVTLAATPGHVHAFDLESGLRLNDKPIVSA"
     gene            2562..3869
                     /locus_tag="VN041_17535"
     CDS             2562..3869
                     /locus_tag="VN041_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HWU30885.1"
                     /translation="MKKQSLRINASSIDPGLLTLPWSTPLGKWPSEHIVSLPKGLSRH
                     LVRFADLSGRVVAVKETTEEMAQREYEMLGSLAKLDVPCVDRVAVIAGRRDSDGRELP
                     AALVTSHLRFSMPYRALFTQVLRPDTADRLVDALALLMVRLHNVGFYWGDVSLSNALF
                     RRDAGAFAAYLVDAETGELHEEGLTDGQRSYDLDLARTNIAGEIMDLAAGGRLERGVD
                     ALEIADGIVSSYRSLWNALTDEESFGVDEAWRITERVQKLNALGFDIDEMSMVTTPDG
                     TAVRIQPKVVDAGHHQRRLIRLTGLDVEENQARRLLNDLDEFRVRSKKDLGDEEIYAH
                     EWLTRVFEPVVRAIPYDLRAKLEPAEVYHQVLEHRWYLSQAQGRSVPLAEVLTSYIND
                     VLRHRRDEATIMGPPTETMSLPVITGSVEVSDDTAAIDWRDLV"
     gene            3915..4514
                     /locus_tag="VN041_17540"
     CDS             3915..4514
                     /locus_tag="VN041_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWU30886.1"
                     /translation="MTPETKTYARTEGRRRQIVAATIQALAEGGYAAMTFSRIREVGR
                     LSSTRLISYHFDTKDRLAEAVVMQVVSDAAEIMRPAMDAAPDLRAKLSAYIRSNLEYL
                     SSHPAEAIAVTEIVRGSGRPRSGEDVSVMLLSVLLSQGQHAGEFGEFDAEVMARSIRA
                     TIDEFAYRRTLSPRDDAAEIAEIVAIYDRAAARAEGNQP"
     gene            4511..4933
                     /locus_tag="VN041_17545"
     CDS             4511..4933
                     /locus_tag="VN041_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30887.1"
                     /translation="MTTDRLLDAGAVRDLDHVKRLWAAAGIVSFLGLAVTAVLAFTDP
                     ADVNWVMWLRGIAVTVACFAFILVTAAVRRGSRRAYTRMRLVATIAPIGVALLIVAPD
                     SGYPGWMKVEQAVLAIPVIVIAVLLWRRRMRQALRPAR"
     gene            4952..>5389
                     /locus_tag="VN041_17550"
     CDS             4952..>5389
                     /locus_tag="VN041_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HWU30888.1"
                     /translation="MSSASYLERLPIPALRGVARTAWIQDTGHAVYQQRHLPTGGVEL
                     HVPVGGDPVLLGALTRPQVELIPAHTTMLGVRFLAGSPLPLPVALDELVDARVLLRDL
                     WGSRADALAEAVALATTSAAALAATGLQCGRSPSARPANHHPGR"
BASE COUNT          839 a         1901 c         1808 g          841 t
ORIGIN      
        1 ccggagatga tctcgggcgg gccttccgct gcgcagagcc ccctgccgga ctcgaaccgg
       61 ccacctgcgg tttacaaggc cgctgctcta ccagatgagc taaggaggcg agggtggccg
      121 aagccgctca taagcatagc gacggccagg acgcgctccg cgcgtcaggg cgtggcggtc
      181 tccgtgggcg cgggcgaagc cggcggcgtc ggagtcgggg tggggtcggt ctcgccgtag
      241 tactcgtcgc tggccgtggt cagcacgaac tgcgagaact ccgcggggtt gttcagcgcg
      301 ccgacatacg cctggccgcc cacgacgatc agcgcctcgt tgttcagctt gaggtccttc
      361 gagcccggca gcggaccctc gagagcgcgg gccgtggcgt ccttcgccca gctcacgaag
      421 tcctgaccct cgatgcagga gcgcaccttc ttggcgttct gcacgccgac ggcgaccgcc
      481 aggtcggcca gttcgacatc cgagcggccg tcgctgtcga tcttgggctg gtcggtgagc
      541 agctcatggt tgtagtcgaa gaactgatcc ggcgagtagg tcgccacgca tgcagcggcg
      601 gccgcagccc gcagcgagta cttcgtgccg ttcgagctgg ccatcaggag cgccaccggg
      661 tgataggtca cggtgacggc gccctgcttg atccagccgg ccagctgacg ggcgttggcc
      721 ttctcgaact cggcggcgcc cgccgacagg tagtcgacgt agatgtggat ctgggtcggc
      781 tccgcggcct tctccgtggt cttgggcgtc ggcgtcgcag ccgcctcgga gggcgtgggc
      841 gacgcgttcg gggagatgtc cgcaccgccg gaggcgaggg tcacgtcgct cacggtcacg
      901 ccgtcgtcgg tcatgccgct cgggctgagc atgggcttgg acatcgagga gctgacggcg
      961 aaagcgacgg ccgtgccgat cgaccccacg gccaccgccg cgacgagccc gatgatcacg
     1021 cgtcgcatga cgcgggcacg cgactgctgc gcatgcactc gctgggcctt ctcccgcacg
     1081 gcttcgcgtg agttgcgagc ggcagggacg ttcggcgttt cgtcgctcga catcgttcct
     1141 cttgaaagat cggatgggac gggggatccc gctgccgcta cgggtgcggc accggatccg
     1201 atgttacgga ggcagtctgg gaaataccca gacgcgcacg cgccgtgcca tactgatctc
     1261 gacccgatgg cgggtcacgg cgggcgaacg cccaccgctt ccatcacaac ggatcgtccg
     1321 gcacgtacct gccggtgaag gagaaacaat ggcaaccgtt acgttcgacg aggccacccg
     1381 catctacccg ggcggcaccc gccccgcggt cgacaagctg aacatcgaca tcgccgacgg
     1441 cgagttcctg gttctggtcg gcccctccgg ttgcggaaag tccacgtcgc tgcgcatgct
     1501 cgcgggcctc gaagaggtca acgccggccg catcctgatc ggcgaccgcg acgtcaccga
     1561 cgtcccgccg aaggaccgcg acatcgcgat ggtcttccag aactacgcgc tgtacccgca
     1621 catgaccgtc gccgagaaca tgggcttcgc gctgaagatc gccggtgtcg gcaaggagga
     1681 gcgcgcgcag cgggtgctcg aggccgccaa gctgctcgat ctcgaggagt acctgaaccg
     1741 caagccgaag gccctctcgg gtggtcagcg tcagcgcgtc gccatgggcc gcgccatcgt
     1801 ccgccagccg caggtgttcc tcatggacga gccgctgtcg aacctcgacg ccaagctgcg
     1861 cgtgcagacc cgcacccaga tcgcgtcgct ccagcgtcgc ctgggcgtca ccaccgtcta
     1921 cgtcacgcac gaccagaccg aggcgctcac catgggtgac cgcatcgcgg tgctcaagga
     1981 cggcctcctg cagcaggtcg gctcgccccg tgacctgtac gagaagccga acaacgtctt
     2041 cgtcgccgga ttcatcggct cgcctgcgat gaacctgttc cccgccgacc tcgccgaggg
     2101 cggcgtgcgc ttcggcaccg aggtcgtgcc gctggaccgc gacacggtcg gccgcgccaa
     2161 cggcagccag gtcatggtcg gcgtgcgtcc ggaggacatc accgtcggac cggccgacgg
     2221 caagggcatc tccgtggtcg tcgacctggt cgaggaactc ggcgccgacg gctacctgta
     2281 cgggcacgcc gacatcaacg gcaagcgcgc cgacatcgtc gcgcgcgtcg acggtcgcag
     2341 ccaccccagc gccggcgaga ccgtcaccct ggcggcgacg cccggccacg tgcacgcatt
     2401 cgacctcgag tcgggtctgc gcctgaacga caagccgatc gtctcggcct gatcatctga
     2461 tcgcagcggc gcgggtggac ctgtatggac cacccgcgcc gcagtgtttc cgcaggacat
     2521 cgcaccgccg gcatccactt ccccacacac gcggaggacg catgaagaag cagtcgctgc
     2581 gcatcaacgc gagctcgatc gatcccggcc tgctcacact gccgtggagc actcctctgg
     2641 gcaagtggcc gtctgagcac atcgtctccc tccccaaagg gctgtcgcgc catctggtgc
     2701 ggttcgccga cctgtccggc cgcgtggtcg ccgtcaagga gaccaccgag gagatggcgc
     2761 agcgcgagta cgagatgctc ggcagcctgg cgaagctcga cgtgccctgc gtggaccgtg
     2821 tggccgtcat cgcgggtcga cgcgacagcg acggcaggga gctgcccgcc gcgctggtga
     2881 catcgcacct gcggttctcg atgccctatc gcgcgctgtt cacgcaggtg ctgcgccccg
     2941 acaccgccga ccggctggtg gacgcgctgg ccctgctgat ggtgcgactg cacaatgtcg
     3001 gcttctactg gggcgacgtg tcgctgtcga acgcactgtt ccgccgcgat gccggggcat
     3061 tcgccgcgta cctggtggat gccgagaccg gcgagctgca cgaggagggc ctcaccgacg
     3121 ggcagcgctc ctacgatctg gatctggccc ggacgaacat cgccggcgag atcatggatc
     3181 tcgccgcggg aggcagactc gagcgcggcg tggatgcgct cgagatcgcc gacggcatcg
     3241 tctcgtcgta caggtctctg tggaacgcgc tcaccgacga ggagtcgttc ggcgtggatg
     3301 aggcgtggcg gatcacggag cgcgtgcaga agctcaacgc actcggcttc gacatcgacg
     3361 agatgtcgat ggtcaccacg ccggacggca ccgcggtgcg gatccagccg aaggtcgtgg
     3421 acgccgggca ccaccagcgt cggctgatcc gcctgaccgg tctcgatgtc gaggagaacc
     3481 aggcgcgccg gctcctgaac gatctggacg agttccgggt gcgctccaag aaggacctcg
     3541 gagacgagga gatctacgcg cacgagtggc tgacccgcgt gttcgagccc gtggtgcggg
     3601 ccatccccta cgaccttcgc gcgaagctcg aacccgccga ggtgtaccac caggtgctcg
     3661 agcaccgctg gtacctgtcc caggcgcagg gccgatccgt gccgctcgcc gaggtgctca
     3721 cgagctacat caacgatgtg ctgcggcatc gtcgcgacga ggccacgatc atgggcccgc
     3781 cgaccgagac gatgagcctg cccgtgatca ccggctcggt cgaggtctcc gacgacaccg
     3841 cggcgatcga ctggcgcgac ctggtctgac ccagacccct tgcacaaatt actcatgtga
     3901 gtaatatctt cagtgtgaca ccggagacca agacatacgc ccgcaccgag gggcgccgcc
     3961 gtcagatcgt cgcggcgacg attcaggccc tcgccgaggg cgggtacgcc gcgatgacct
     4021 tctcgcgcat ccgcgaggtg gggcgcctca gcagcacccg gctcatctcg taccacttcg
     4081 acaccaagga caggctggct gaggccgtgg tgatgcaggt ggtgtcggat gccgcggaga
     4141 tcatgcgacc ggccatggac gccgcaccgg atctccgcgc caagctgtcg gcgtacatcc
     4201 gctccaacct cgagtatctg tccagccatc cggccgaggc gatcgccgtc accgagatcg
     4261 tccgcgggag cggccgcccc cgttctggcg aagacgtctc ggtgatgctc ctctccgtcc
     4321 tcctctcgca gggccagcac gccggggagt tcggcgagtt cgatgcggag gtcatggcgc
     4381 gcagcatccg ggccaccatc gacgagttcg cctaccggcg caccctctcc ccccgcgacg
     4441 acgccgcgga gatcgccgag atcgtcgcca tctacgatcg cgccgctgcg cgcgcggaag
     4501 ggaaccagcc atgaccaccg accgcctcct cgatgcgggt gccgtccgcg acctcgacca
     4561 cgtgaagcgg ctctgggccg cggcggggat cgtctcgttc ctcgggctcg ccgtcaccgc
     4621 cgtgctggcc ttcaccgacc cggccgacgt gaactgggtg atgtggctgc gcgggatcgc
     4681 cgtgaccgtc gcgtgcttcg cgttcatcct cgtgacggcc gcagtgaggc gcggcagtcg
     4741 tcgcgcctac acccggatgc ggctggtggc gacgatcgcg ccgatcggcg tcgcgctgct
     4801 catcgtcgcc cccgactcgg gatatcccgg ctggatgaag gtggagcagg cggtcctcgc
     4861 gatacccgtg atcgtgatcg cggtgctcct ctggcgccgc aggatgcgtc aggctctgcg
     4921 gccggcgcgc tgagacgacg ggtacccgcc cgtgtcctcc gcctcctacc tggagcgcct
     4981 cccaatcccg gcgctgcgcg gtgtcgcccg cacggcctgg atccaggaca ccgggcatgc
     5041 ggtgtaccag cagcgacatc tgcccaccgg cggggtcgag ctccacgtgc cggtcggcgg
     5101 cgatccggtc ctgctcggcg cgctcacccg cccccaggtg gagctgatac ccgcacacac
     5161 caccatgctc ggcgtgcgct tcctcgccgg ctcgccgctc cccctgccgg tcgccctcga
     5221 cgagctcgtc gacgcccgcg tgctcctgcg cgacctctgg ggatcgcgtg cggacgcgct
     5281 cgcggaggcg gtcgctctcg caaccaccag cgcagccgca ctcgcagcca ctggacttca
     5341 atgtgggcgc tccccaagcg ctcgacccgc caaccatcac ccaggtcgt
//
LOCUS       DATMSX010000555        39239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_169198,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000555 DATMSX010000000
VERSION     DATMSX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 39239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39239
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169198"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VN041_17555"
     CDS             complement(<1..1649)
                     /locus_tag="VN041_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWU30889.1"
                     /translation="MRSSAPLLRVRDLSVTHADAARSSPQQVSFDVHAGEVVILMGPS
                     GSGKSTLTLALNGLIPQSVEAEMTGTVEVGGRDTRTTPVAQLSTDISMVFQDPDSQLV
                     TGTVLDEVAFALENLLLRASEVLERAEDALRRVGLWHRRSWNPDLLSGGGRQRLAIAC
                     ALAMRSRVIVLDEPTANLDPQGVDDVYAALAALTTDGRERAIVLVEHNLEAALRFATR
                     VIVLDDEGRMLWDGPAHEVLEEHAVELEELGVWGPRSLNELPRHDGPEAPQAAITVRE
                     LVVEKRGTEVLSIPALDIVAGSFTAIVGANGAGKTTLLQALGGVVAPPRGTVMIGDLD
                     AGRASPRALSSQVGFVFQNPEHQFIAHTVREELAHGLRLQRVGSTEIGARVDEMLDRF
                     GLRYRADEHPFLLSGGEKRRLSVGTALITRPRVVALDEPTFGQDRARALELLSLLQDL
                     QRSGTTVVVVTHDLDLVAAYATHTVLLDAGALVDAGPTAEVLAGLSDGGSADAEPAAS
                     ATPAGTLSQKAPLPGHPTHQTWDADTARVAPSAPHSPATEPT"
     gene            complement(1730..2275)
                     /locus_tag="VN041_17560"
     CDS             complement(1730..2275)
                     /locus_tag="VN041_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWU30890.1"
                     /translation="MPPAASLSTRTLLVCAAIGVATGILGGIAGVVTLGVLAVTPYLY
                     GLVLGVHVLPGIIAQEVLRRPLVALITHVIAALISSAFNPAWAMRFIGTALLFGAIQE
                     GVAALTRYRAWGRWRFFVSAVIIGAIVAVVVGFVVDLGRFDLWVQVVYLTISVLGPVA
                     WTAVGIAVGNALRRAGIAPKR"
     gene            2520..5456
                     /locus_tag="VN041_17565"
     CDS             2520..5456
                     /locus_tag="VN041_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWU30891.1"
                     /translation="MTPTPVDGTPGHYIVLMKADPLASYDGGTAGFKATKPAKGDKLD
                     AHSANSQKYIAHLKNEQRKLAAEQGVTPDADYQVVANGFSAQLTGAQVDKLRASKDVL
                     GVYADEVRHPQAQTSTDFLGLGDDATGKGGVWEKTGGVGTAGEGIVVGVVDTGIAPEN
                     PSFAGAKLKNGNSPKSKGQPYTDGKYVYFDKADGNTFKSPIVSGQEWDKHDYSTKLVG
                     GQYFSAGAKAAGFDFQYDLLSPRDGAGHGSHTSSTAAGDFGVKASVSGVDFGTVSGVA
                     PAAKVAMYKACYEGPDPAVTTDDICALSDLVSAINAAVTDGVDVINYSIGGGAATGIL
                     EPDDIAFFNAAAAGIFVSVSAGNDGPDPVTADHASPWYTTVAASTIPTWEGTVKFPGF
                     EKAGASVSVPFGSEVTGPSIYAGDAAAGADANLCLPGTLDAAKVKDHIVVCDRGSNAR
                     AEKSEVVKAAGGIGMVLVNVPGGADSLDNDFHLVPTVHLAAANRAAVLAYVQSGTDLP
                     ITLIGENTTGVTTPVPQIAGFSSRGPMNAAGSDIIKPDVAAPGVAILAATNNRQDEQP
                     TFNILSGTSMASPHVAGLAALYLGEHPDASPAEIKSALMTTAYDTVNADGSRSTDVFA
                     QGAGQVDAKRYLQPGLLYLNGAQDWAEYIEGVLPGTFDDVKPIDASDLNVASIAIGTL
                     ASEQTVTRTVTATAPGMYTAVASIPGVKVTVEPSVLSFAKAGESKSFTVTFDNSSAPV
                     EKWATGSLTWKGQSGDVRSPLAVFPVTAAAPAEVSGMGVDGSASVKITSGVNGTMALN
                     VNGLAPLQLLTDPDNPVDGHSGDGKSGDENKDVSWIVDVPAGTTLSRFDLDSSDDAGS
                     DLDLTVYRVVSPTDLRYYQKWQSATASADERVTIPAPTAGTYMVVANIYSAPNPMTWD
                     MTYANVAPGAGQGAFTATPNPLTVTRGEDVSYDLSWSGLAAGKYLGVVQYGDSDVRTI
                     VTVDAK"
     gene            complement(5520..5816)
                     /locus_tag="VN041_17570"
     CDS             complement(5520..5816)
                     /locus_tag="VN041_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWU30892.1"
                     /translation="MPDITVSRNDDASRYEIRLDSTLAGYAEFDRRPGEFRFTHTEID
                     HAFQGRGLAQQLAAEALADAATTGDAIAPYCPFIASYLTKHPLDGVEIRLPNQG"
     gene            5920..7929
                     /gene="recQ"
                     /locus_tag="VN041_17575"
     CDS             5920..7929
                     /gene="recQ"
                     /locus_tag="VN041_17575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HWU30893.1"
                     /translation="MPTPSDPYDDIPYPDEPWGEPEEMDWVPPEDGWEPPLDWGSPAE
                     AAPSPSNGSGTASVPTAAKAAPSRFGTALEALRTVFGYDAFRGDQAAIVEQVIGGRDA
                     VVLMPTGGGKSITYQVPALVREGTGLVISPLIALMHDQVDALRANGVRAAYLNSTQAI
                     DERREVERAYVAGELDLIYVAPERLSSAQTTQLLQRGTLSVIAIDEAHCVSQWGHDFR
                     PDYLALGDLAERFPGVPRMALTATATKATHQELTERLHLDRAAHFVASFDRPNIQYRI
                     VPKVDPRKQLVEFIRSQPESAAGIVYALSRKSVEQTAEYLASRGFDALPYHAGLPAEV
                     RAANQSRFLREDGVIMVATIAFGMGIDKPDVRFVAHIDLPKSVEGYYQETGRAGRDGE
                     PSVAWMAYGLGDVVQQRRLIDQSPGDRTFKMRMGQHLDAMLALCETVACRRQNLLRYF
                     GQDSEPCGNCDTCLEKPDTFDGLVPAQKLLSTIVRLKRERNQAFGAGHLIDILRGSSN
                     DRIRKMRHEQIATYGIGADLSDQDWRSVVRQLLARGILVAQGEYGTLAPGDAAGGVLR
                     GDTPVPLRKDTIGRVTASRTRKTSAADALDAEDQPLFEALRTWRAATAREQGVPAYIV
                     FGDATLRALAEHRPASVDALSGISGIGEKKRDAYGEAVLEVIAAH"
     gene            complement(8045..8455)
                     /locus_tag="VN041_17580"
     CDS             complement(8045..8455)
                     /locus_tag="VN041_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWU30894.1"
                     /translation="MSARYRTEASNGVDGRSRVPDGLDVAVASPLKADADPAATNPEQ
                     LLALAWATCLNGTAQVLVREQRRTHVRVEVELHPVAPGPGYAFHVDAFLSAEGMTDAE
                     AQDLLERAHARCPVSKLIGGAATVHVHTEEFAAA"
     gene            8559..9989
                     /locus_tag="VN041_17585"
     CDS             8559..9989
                     /locus_tag="VN041_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30895.1"
                     /translation="MSDLRTTTPTSIRTARDVHALLAEKGSQGARTKAIIVLALGGIF
                     MDAYDFSSLAFGITAVKEEFGLDPLMTGVVNAAIMVGSVVGAIFGGMLVDRFGRYKLF
                     MADMAFFVVAAIGCALAPNEWVLILFRFVMGIGVGLDLPVAMAFLAEFSKLRGSGSRS
                     QRVNAWSPAWYIATGFGYLLVLAVFLLLPVAQHGILWRVVVGFGAVPAIIVLLVRRKY
                     MAESPQWLADQGDLRGAVDVMRSHHGLDVELAPDADTARTTKARTGHWRQYGELFAAR
                     YRLRTIAALCVSVFSTFGYNAVAYGTPLIIAMLFQQGPLVTILSALVINLGFGAAGGL
                     LGVGLINRVGARRMTIIGFAIQAASLLILAFVGIPTGALVIISIAMLAAFVFAQAGGP
                     GANLMSYATLSYPTRLRGVGVGFNEAVKRVFSIVSLVFFPVLAAALSTGVFWIVALAP
                     LAGLISLLVIRWDPTGKDVDAEDQEA"
     gene            10145..12205
                     /locus_tag="VN041_17590"
     CDS             10145..12205
                     /locus_tag="VN041_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWU30896.1"
                     /translation="MVDAAVRPTAASAPEAGIDVARVNELLMGTWAHTRREARELIKD
                     PAFWRDDSLGKDEHRERVLTQLHLLVENNAVHRAFPKAFGGEEDNGANIAGFEELVAA
                     DPSLQIKSGVQWGLFGGAVLQLGTEEHHKRWLPGIMDLSIPGAFAMTEIGHGSDVAAV
                     GTTATYDPQTEEFVINTPFRAATKEYLGNAALHGIAATVFAQLITNGVNHGVHCFYVP
                     LRGEDGEDLPGIGREDDGLKGGLNGIDNGRLSFDHVRVPRTNLLNKYGDVAADGTYTS
                     PIDSPGRRFFTMLGTLVQGRVSLDGASSWASALGLHIAITYATQRRQFDGADGQETVL
                     MDYGKHQRRLLPRLATTYAQIFAHDEFLQKFDGVFSGRTDTPEDREDLETLAAALKPL
                     STWHALDTLQEAREACGGAGFMFENRLVGLRADLDIYATFEGDNNILLQLVGKRLLTD
                     YARQFKAKDAAALAKYAVGQTADRVFHGAGLRALGQAVADLGSTARSVENGLRAEQQH
                     ELLTERVQQMVADIAGRLRPAGKDRALGEKLFNQNQAELIEAARAHGELLQWEAFTDA
                     VNAIEDEDTRQVMTWLRDLFGLTLIEKHLAWHLINGRLSTQRAAAVSSYIGRLCHRLR
                     PHALELVGAFGFEPEHVRAPIASGIEQQRQDEARAHYAALAASGEAPISEKELRKQAK
                     QKNG"
     gene            12360..12758
                     /locus_tag="VN041_17595"
     CDS             12360..12758
                     /locus_tag="VN041_17595"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWU30897.1"
                     /translation="MSNVTQIGELASWGGEKGKQFLDKEIGTEFAGVSVNTTAPGSES
                     PFWHAHAKIEEIYIVVDGRGEFALDDEVLPLEAGTAVRVAPGVMHALRALPESPSPMR
                     WLCIRAAGQELASVGHDAELDRERPFPWNA"
     gene            12762..13337
                     /locus_tag="VN041_17600"
     CDS             12762..13337
                     /locus_tag="VN041_17600"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWU30898.1"
                     /translation="MASVLTGPDGRGRCAWVGDDAEYRRYHDEEWGTALHGDRALFEK
                     MSLEGFQAGLSWITILRKRPRFREVFAGFDPALVAEFGEEDVERLMADAGIIRNRAKI
                     LATISNAALVREMADGELDALMWSFAPAASPRPRTFDDVPAVTAESTALSKALRKRGF
                     RFVGPTTMYALMQSAGMVDDHVDGCWRASGR"
     gene            13458..19541
                     /locus_tag="VN041_17605"
     CDS             13458..19541
                     /locus_tag="VN041_17605"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU30899.1"
                     /translation="MRVLSWMRARPKRLAAGAGVAIAAVALTGMAVAYDGLPTTKVDL
                     NDAGVWLTKSSSLLVGHFNHESTVLDGGLRTSSESYDVLQDQASVLIVDDGSGTMSPV
                     DPARVSLSGAVKLPGDAQVALRRNTVAIMERSSGRLWVMPFSGVANFEPGSAEPTATL
                     GKNAAVTVGADGTVYGVAGGKGEILTVTTDAQGLPLEEKRESVGKIDGAARPTVTAVG
                     STPVVLDSSGTVIAPGGVRAEVASDGSAVLQQASTETGAVTVATSNALVRVPLDGAEP
                     TRTTVPSTGNPAAPVFLDGCTYGAWAGSARFIRDCAGKSSDVATKVPGAEASTQLTFR
                     VNRDVIVLNDIIGGAAWLADESMQRVDDWSILTPPEGEAEDEEKTTEETVETTLPERK
                     EENTPPTAEDDPGLGVRPGSTTMLSVLDNDNDPDGDVLVASLVGSQPSVGEVQPVLDG
                     AALQIAVPEDATGTASFQYQADDGRSGTDTATTTVSVHAWSENSAPKTKRVTKLAVES
                     GGTISYNVLPDWIDPDGDDIYLREVVAAPGDEVEFTTGGQITYRAVGSLQGRKEVQVT
                     VSDGLGEEANGTILLDVQPPGSTLPKTNADHVVTRPGVAVTVSPLTNDASSGREQLRL
                     AGVDDVDGAVISSDLPNKQFTFTSQTAGVYYVQYLVAAGPNAVPGLVRVDVLDTADSE
                     LPPVAVRDVALLPAGGEVLLDPLANDSDPSGGVLVLQSASLETDSRGISVSVLEHQTL
                     RIGDQGGLSGPARITYRISNGTRSAEGEVVVLPIPAPDRILAPVAVDDQAVVRAGDVV
                     TIPVLANDSHPSGDAIHVVPALVEPLVDPADGEIFVSQDTVRFRAGPEPKTVNATYEI
                     IDSMGQKAAAYIRIQIVPIDAEKNAAPVPRDLTVRTLSGATQRIAIPLDGLDADGDSV
                     ELLGLASSPQKGRVVETGQDFLSYEAFDDATGVDSFTYRVRDRLGREGTGTIRVGIAP
                     RETVNQAPYAVKDSVVVRPDREVAVPVLINDSDPEGGKISLVKNGVSQPDVDGLKARV
                     SGDRLLIRVPDRELETSLRYTIVDELGATATAVVQVTVDEDVPLKAPIARDDRVLVTD
                     VKDSATIDLEVLANDEDPDGTTQALSLEAGAGGTVRPEGKVRVTVGEKAQIVRYTVTD
                     RDELQASAFIYVPALSDMRPVLSRVEPVTVKSGETKVLPLKDYVTVFGGGRAIITEAA
                     KVVAPHSNGANLIQDESTLVYTSAEGYFGKDSLTFEVTDGTRVDDPKGRKATLTIPIT
                     VLPPDNQPPTFTDAQMQVAPGEDATILDLAALASDPDPGDKVSFRLTGGAGEGITARI
                     DGTKLSVEAAKDVRKGTVANLEITATDGTTEPVIGRVVVTVTASTRDLAIATDDVLDE
                     VDQGKPLTVPVLENDINPFPDTPLKMVSASLETGRGEVTTKGDQVVVTPAADFVGVLV
                     VRYRIQDATKDPDREVDGRVRLTVQGVPDAPGTPRVTSVQDRTVVLSWASPVNNGAEI
                     TKYTVRSVQGGAYSKDCESTTCTLDRLTNNVEYVFQVTATNRVGESKPSPSSEVARPD
                     TRPDAPSAPRLAFGDHELKVAWTTPSTSGSPVESYTLQISPAPPSGPAEISGVTGTST
                     VWKGLENGRAYQVRIRAHNRAPEPSDWSLYSLAETPAGPPMAPAQPTTAELDPVGSKA
                     QIQVNWVAPDSNGDKISGYELQVLQGSTVVRTLKPAAGATSQAVQVDPSETGYTFRIR
                     ALNKAPLPGEWSPPSAERRGVLAPGAPTSVKATPGDNTVTVAYTPGPLNGAKASEVTY
                     QYRVGAGGSWNAMPGDRVIRSGVNNNGTYQIYVRAISNVAGTTSKAGPASAASNDVAP
                     YGRPNVPSLSASVSGQTITWHIGATARNGRDFTVTWADNRGHSGSIGAGGGNVPLSYD
                     WSTPVSFTVTVTDKPSALQPAGAQTNAVTGNSGTGPKPLPEVWVSRGGPATWGPSTCQ
                     NVRVTVSSDFPAGRYVVDAYNNGVWFGNNKGNAVQVPGDLGSYVCPGEAGHTISVSIQ
                     GVNATVHPVIW"
     gene            19685..25783
                     /locus_tag="VN041_17610"
     CDS             19685..25783
                     /locus_tag="VN041_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU30900.1"
                     /translation="MRARPKTLAATAGVLAGTVVLTGMALAYDGQPTTKVDLNDAGVW
                     LTKTSSLLVGHFNHESTVLDGGLRTSGENYDILQAGATVLVADQGNSTLTAIDPANVA
                     LTDSAAIPSDAKVALGSHTAAVLDRKSGDLWVVGASSVSGFDVKGTKPLAELGKDADV
                     AVAQDGTVFGLSVERGEIVTIPVDRQGDPLAASTASVGEIGASPRPSITAVGSTPVVL
                     DAAEGVVSTPGGIRVDLADLGAGSLKDAVLQQASAPADAVALATGQTLVEIPLDGAKP
                     VVRKAGGTGIPAAPVFLQGCTYGAWGGSGRFLRDCAGDADDVAADIPGAEQSTHLTFR
                     VNRDVIVLNDVVGGEAWLADDSLQKVDDWSILTPPEGDAEDEEKTTEETVETSLPERK
                     PENTPPVAEDDPDLGARPGGTTLLPVLDNDNDPDGDVLVATLDDTQPQVGEVQPILNG
                     AALQILVPDDAKGTATFRYKVDDGRPSGTDTANATVTVYPWDVNTAPKPKRKAKLSVE
                     SGGTLSYGVLADWIDPEGDDLYLLNVTAAPGDEVDFTTDGQMTYRAVGSLQGRKEIEV
                     VVADALGETATGKITLDVKPAGTTVPVTNADHVVTRVGETATVSPTANDTSTGRDELR
                     LTRVEPLDGVQIVPDYPKKQFGFKASAPGVYYVQYLVSAGQADAKGIVRVDVLDEKAS
                     ELPPVAVRDVALLPKGKEALVDVLANDSDPAGGVLVVQSVTVPPHSGIDVSVLNHSTL
                     RIGDQGALDQPVKIRYTISNGSKSAQGDVTVIPIPAPTKLAAPVTLDDEAVVRVGDVV
                     TIPVLDNDVHPNGDVLHVAPDLIEPLVDPKDGEAFVSQDSVRFRAGSEPRTVYLTYEA
                     VDSMNQKAGGHVTIQILPVDEEANAAPRPRDLTARALSGGSIRIPIPLDGIDADGDSV
                     ELLGQDVAPVKGRISETGSNFLEYQAFADATGVDTFTYRVRDRLGAEGTATVRIGIAP
                     PESTNQSPYAVKDAVIVRPGRSVAVPALANDSDPDGDEIRLVKDGIEVPADTGITAKA
                     SGDRVVVKVPDHELEASLQYTIRDARGAEATAPILVKVDKNVPLMTPIARDDRVRVED
                     LKDSLTTDVDILGNDEDPDGTVEGLKVAVEDGGRLLGDRKVRVTVTDKRQLVRYTVTD
                     QDGLTASAFIFVPSISELRPVLRSTKPVEVKSGETKELKLSEYVMVAGGGAVRLTEAS
                     KISAGHGNGDSLIKDETTLVYTSKKGYFGDDAVSFEVTDGKTVEDPAGRKSTLSIPIT
                     VLPPENQPPVFVNGQVDVAPGEKATPLDLAALTTDPDKADADRIQYRITGDPGEGVSA
                     RLDGKRLLVEAGTSTPKGTSTSVRLSITDGTTEAIEGTVAVRVIASTRSLPVASPDTI
                     DEADQGKSYRIPVLENDINPFADEGTPLKVLSATVESGDGGANVVGDQVEVTPGKKFV
                     GVMVVRYRIQDATKDPDREVDGRITMTVQGVPDAPGVPRVTTVEDRTVVLNWSAPSNN
                     GAEIDHYTVTSVGGRPYTKTCESTTCTLDGLTNNVEYTFKVTAHNRVGDGPESPVSEP
                     ARPDVRPERPLPPTLVFGDQSLKVSWTTPQSNGSPVDSYTLQISPAPLRGASEKTGVT
                     GNSIVWDGLENGTSYQVRVQAHNQAPDPSDFSVYSQPEIPAGKPGTVAAPTVSSAPSV
                     GSEAQMTVRWADADPNGDAVKNYDLLVYRGGSLFQTLAVGTATSKTVSVPTNSSDYTY
                     AVKATNKAGTSALSPQSTPQRAFGTPGAPTNVVAKEGDTQITVTFTKPDFNGAKDSEI
                     RYQYSLNSGTWTNWNGTSAIPANNGTAYTVKLRAYSVVGSQQSQPGKESAPSNQVTPY
                     GAPHAPTGSATNLGQAIRLAWNASGSDNGRPVTTYISIDGGGWQQVAISGSTDVGNGY
                     SQTHSIRVRAVANPGGEALSPVYSATTNPPPQPSAQTVRGTLAVDHGWLNGYSCAQDT
                     CYYVAADTANFPAGNYRVTCYANGSAFSSSYSTQYVPANGRVQATCWTGGNSGGDYNI
                     QLYIEGWGLAAPTNWQ"
     gene            25914..31982
                     /locus_tag="VN041_17615"
     CDS             25914..31982
                     /locus_tag="VN041_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU30901.1"
                     /translation="MTWVRARPKTLAATAGVLVGSVVLTGMALAYDGLPTAKVELNDA
                     GVWLTKSSKLLVGHFNHESTVLDGGLRTSSENYDILQNGPTVLVVDHGNSTLTAVDPA
                     NVSLTDSATIPGDAKVALGDRTAAVLDRASGSLWVLGASEIAGFDIKGADPVAKLGAD
                     ADVTVAHDGTVFAVSPKDGDIVTVPVDDQGAALDPKTSALDGVKGASEPSITAVGDVP
                     VVLNPSDGTVLAAGVRTELKGAKDAVLQQTSAATDAVAVATSAGLVRVPLHGGEATTV
                     KAGGTGTPAAPVFLEGCTYGAWGGSARFVRDCAGERDDVTAKIPGAESSTRLMFRVNR
                     DVIVLNDVIGGEAWLADESLQRVDDWSILTPPEGETEDTDESTQETVETSLPERKEEN
                     TPPVAEDDRLGVRPGGTTLLPVLDNDNDSDGDVLVATLAGAQPSVGEVQPILNGAALQ
                     IMAPDDASGTAAFTYQVDDGRANGTDTATVTATVYDWDTNTAPKQKRKPKTSVESGGT
                     LSYNVLQDWIDPEGDDLYLKSVVAADGDEVEFTTDGQMTYRAVGSLQGRKEIEVAVAD
                     ALGEVATGTIILDVKPAGTTAPVTNADHIVTRVGETATVAPLANDSSSGRDELRLTRV
                     EPLDGVTVLPDYPKKQFAFKSNAPGVYYVQYQASAGQADAKGIVRVDVLDKQESELPP
                     VAVRDVALLPTGKEALVGVLANDSDPAGGVLVVQSVSVPPHAGVAVSVLNHETLRITD
                     QGALDEPVKITYRISNGSKSAEGDVTVVPIPAPSKITPPVTHDDEAVVRVGDVVTIPV
                     LANDVHPNGDVMHVAPDLVEPLVDPKDGEAFVSQDTVRFRAGKQPGTVYLTYEAVDSM
                     GQKAGGHVTVQVLPVDGEVNAPPRPRDLTARVLAGTSIRIPVPLDGIDVDGDSVELLG
                     QDIAPTKGRITETGGDFLEYEAFKGSTGVDTIVYRVRDRLGGEGTATIRVGIAPAEAT
                     NQAPFAVKDTVVVRPGRSVAVPVLANDSDPEGDEIRLVKDGIETPKDSGITARASGDR
                     VVVKVPDRELDASLQYTIRDSKGAEADAPILVTVDKDVPLMTPVARDDRPRVEDLEDS
                     LSADIDILANDEDPDGTVEGLKVAVDGGAKLVGDRKIRVSVTDQRQLIRYTVTDQDEQ
                     TASAFIFVPALSELRPALRSTKPVEVKSGETKVLELADYVTVAGGGNVRLTEASKVSA
                     GHGNGDTLIQDETTLVYTSEKGYFGKDSLSFEVTDGKTVEDPEGRKSTLSIPITVLPP
                     DNQPPVFVNGQIDVAPGEEATSIDLAALTTDPDKGDAGKIRYSITDRPGDGLNARLDG
                     SKLLVDASATTPKGTTATVGLSITDGATEAVEGSVTVTVTASTRPLAVAGPDNVDEAD
                     QGKTITVPVLQNDINPFEADGKPLKVLSADRESGNGDARVVGDRVEVTPASDFVGVMV
                     VRYRIQDATKDADREVDGRITVTVQGVPDAPGVPRVTTVEDRTVVLNWSAPSNNGAEI
                     DHYTVTSAGGRPYTKTCTSTTCTLDGLTNNVEYTFRVTAHNRVGDGPASPASEPARPD
                     VRPETPLPPKLVFGDKSLQVWWTPPQSNGSPVDAYTLQISPAPLSGSSERKVGNVTSL
                     TWDGLENGTNYQVRVQAHNQAPDPSDYSVWSLGETPAGVPGTVAAPTVSSAPSVGSEA
                     QMTVRWADANPNGDAVKNYDLLIYRGGSLFQTLAVGTATSKTVSVPTNSSDYTYAVKA
                     TNKAGTSAASPQSTPQRAFGSPGAPTNVKATPGDQKIAVTFTKPDYNGAKDGEVRYQY
                     SLSGAAWTNWNGSSTIPANNGTDYTVKLRAYSVVGGQQSQPGAASAASNTVRPYGTPH
                     APTGSASQSGDQQIKLTWNAGGSDNGYPVTTYISVDGGGWQKVATTGSRIVGNGYNQT
                     HTIRVKAVANPGGESLSPTYSATTSAPPSPKIWVTKGDYVSGCVNGCYKFKVNWENAN
                     LGSVTVKCMKDGGQVGNYAHPVTMNGTGSSPSLDCWQGSDNVNVWVDVVGWGDSVDTE
                     KRYWAK"
     gene            32125..38199
                     /locus_tag="VN041_17620"
     CDS             32125..38199
                     /locus_tag="VN041_17620"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU30902.1"
                     /translation="MRLLSWVRARPKALAATAGVVVGSVVLTGMALAYDGLPTAKVDL
                     NDAGVWLTKSSALLVGHFNHESTVLDGGLRTSGENYDILQQSQTVLVVDHGNSTLTAI
                     DTANVSLTDSTTIPGDAKVALGDHTAAVLDRKSGALWVLGAADVAGFDVKATDPIAKL
                     GKDADVTVGQDGTVFAVSAEDGEIITIPVDAQGSPLEPKTSSLDGVKDASEPTITAVG
                     DVPVVLNPSDGVVAAPGGVHARIDDAKDAVLQQASAATDAVAVATPTGLIRVPLNGGE
                     ASTVKAGGSGIPAAPVFLQGCTYGAWGGSARFLRDCGGKQDDVSAKIPGGERSSRLMF
                     RVNRDVIVLNDVIGGEAWLADDSLQRVDDWSILTPPEGEAEDQEDTTQETVETSLPER
                     KPENTPPVAEDDKLGVRPGGTTMLPVLDNDNDPDGDVLVATVAGAQPSIGEVQPILNG
                     GALQIMAPKDASGTASFTYQVDDGRENGTDTATVTATVYDWDTNSAPKPKRKTKLSVE
                     AGGTLSYNVLPDWIDPEGDDVYLQQVVAAPGDEVEFTTDGQITYRAVGSLQGRKEVSI
                     VVADALGETANGTIQLDVKPAGGTVPVTNADHVVTRVGETATVSPTANDTSTARDDLR
                     LTRVEPLSGVTIVPDYPKKQFTFESNAPGVYYVQYQVSAGQADAKGIVRIDVLDKQES
                     ELPPVAVRDVALLPKGSEALVGVLANDSDPAGGVLVVQSVTVPPHSGIDVSVLNHETL
                     RIGDQGALDQPVKITYTISNGSKSAQGDVTVIPIPAPSKIAAPVTHDDEAVVRVGDVV
                     TIPVLENDVHPNGEVMHVAPKLIEPLVAPKDGEAFVSQDTVRFRAANQPGTVYLTYEA
                     VDAMGQKAGGHITIQVLAKDADVNAPPHPHDLTARALAGTSIRIPIPLDGIDTDGDSV
                     ELLGQDVAPIKGRITETGGNFLQYDAFQGSTGVDTVVYRVRDRLGAEGTATIRIGIAP
                     PEATNQAPYAVKDAVVVRPGRSVAVPVLANDSDPEGDEIRLVKDGIEVPEDSGMTAKA
                     SGDRVVVKVPDKEMEASLEYTIRDAKGAEATAPILVTVDKDVPLIPPIARDDRLRVED
                     LKDSLTADIDILANDEDPDGTVDGLKVTVDAGAKLVDARKARVTVTDQRQLIRYTITD
                     QDEQEASAFIFVPSLDELGPALRSTKPVEVKSGETKQLKLSDYVAVAGGGSVRLTEAS
                     KVSAAHANGDNLIKDETTLVYTSKKGFFGDDALNFEVTDGKTLEDPKGRRSTLSIPIT
                     VLPPENQPPVFVNGQVDVAPGEKATSLDLAALTTDPDKADAGKIQYRISKQPGDGINA
                     RLDGTRLLVDASSSTPKGTNAMVSLSITDGTTEPVEGNVAVNVTASTRSLAVASPDTI
                     DEADQGKTYKIPVLTNDINPFADEGTPLKLLSAERESGDGTARVVGDQVEITPGAKFV
                     GVLVVRYRIQDATKDPDREVDGRITVTVQGVPDAPGVPRVTTVEDRTVVLNWSAPSNN
                     GAEIDHYTVTSVGGRPYTKTCQTTTCTLDGLVNNVEYTFKVTAHNRVGDGPASPLSEP
                     ARPDVRPETPQPPKLAFGDKSLQVSWTPPQSNGSPVDSYTLQISPAPLSGSSERKVGN
                     VTSLTWDGLENGTNYQVRVQAHNQAPDPSSYSIWSLGETPAGKPGTVAAPTVNSAPSV
                     GTEAQMTVRWTAADPNGDAIKNYDLLVYRGGALYQTLSAGTATSKTVTVPTNSSDYTY
                     AVKATNKAGTNDAGPQSAPQRAFGKPGAPSNVVIREGDRQVAIASYTKPDYNGANESE
                     IRYQYSLSGGTWTNWDGSSVIAADNGNGYTVRLRAYSVVGGQQSQAGDPSAPSNNVIP
                     YGAPFAPTGSASKSGDKAILLTWNANGSYNGRPITTYINVDGNGWEQVAKSGSRTVGN
                     GYDQTHKIRVKAVASEGGEALSPVYDATTSPQPIKASLGPGAAVSGCSAVGGGSCHKF
                     RITTTSAFKAGTYTVKCFAGGSEFGGYALKIPSGSWVDVDCYNGFSRDKMAKIVGGDP
                     ASISATFG"
     gene            38250..39221
                     /locus_tag="VN041_17625"
     CDS             38250..39221
                     /locus_tag="VN041_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWU30903.1"
                     /translation="MTMTPEQAAWFQSTFTRLVENIDKALQGKPEVVSLVLSAMLAEG
                     HVLLEDAPGTGKTSLAKALAATVQGTNSRIQFTPDLLPSDVTGVTIYDQQNHKFEFHK
                     GPIFASIVLADEINRASPKTQSALLEVMEESRVTVDGVTHEAGRPFLVIATQNPVEQA
                     GTYKLPEAQLDRFLIKTSIGYPSLAVTESILAGAAQRNPSAGLAAVITTSAVADMADL
                     AATVHVEPAVLRYAAELAEATRGDSAIKLGVSVRGAIAMIRIAKVWAAAHGRHYVLPD
                     DIKALARPVWQHRLMLDAEAEFAGTTADTVIARVLDTVAAPQARTNA"
BASE COUNT         6612 a        13790 c        13445 g         5392 t
ORIGIN      
        1 gtcggctcgg tcgcggggga gtggggtgcg ctgggggcga ctctcgcggt gtccgcgtcc
       61 caggtctggt gggtcggatg ccccggcaga ggcgccttct gggacagggt gccggccggt
      121 gtcgcagagg ccgcgggttc ggcgtccgct gacccgccgt cactgagacc cgcgagcacc
      181 tcggcggtcg gcccggcgtc gaccagagcc cccgcatcca gcaggacggt gtgggtcgca
      241 taggcggcca cgaggtccag gtcatgcgtg acgaccacga ccgtcgtgcc cgagcgctgc
      301 agatcctgca gcaggctcag cagctcgagc gctcgggccc ggtcctgccc gaacgtgggt
      361 tcgtcgaggg cgaccacgcg tgggcgggtg atgagcgcgg tgccgaccga gaggcggcgc
      421 ttctcgccgc cggagagcag gaacggatgc tcgtcggccc tgtaccgcag gccgaaccga
      481 tcgagcatct cgtcgacgcg cgcgccgatc tccgtcgagc cgacgcgctg cagccgcagc
      541 ccgtgcgcca gttcctctcg cacggtgtgc gcgatgaact ggtgctccgg gttctggaac
      601 acgaatccga cctgcgagct cagcgctcgg ggcgaggccc gtccggcgtc caggtccccg
      661 atcatgaccg tcccgcgcgg aggcgccacg accccgccca gtgcctgcag cagcgtcgtc
      721 ttccccgcgc cgttcgcccc gacgatggcc gtgaaggacc ccgcgacgat gtcgagggcg
      781 gggatgctga ggacctcggt gcccctcttc tccacgacca gttcgcggac agtgatggca
      841 gcttgcggag cttctggtcc atcgtggcgc gggagctcat tcaggctccg cggcccccac
      901 acgcccagct cctccagctc cacggcgtgc tcctccagca cctcgtgcgc cggcccgtcc
      961 cacagcatcc gcccctcgtc gtcgagcacg atgacgcgcg tcgcgaaccg cagcgcggcc
     1021 tcgagattgt gctccacgag cacgatcgcg cgttccctgc catccgtcgt cagcgcggcg
     1081 agcgcggcgt agacgtcgtc gacgccctgc gggtccaggt tggcggtggg ctcgtcgagc
     1141 acgatcaccc gcgagcgcat cgcgagtgcg caggcgatcg cgagccgctg ccggccgccg
     1201 ccggagagca ggtcggggtt ccagctgcgg cggtgccaga gacccacgcg gcgcagtgcg
     1261 tcctcggcgc gctcgagcac ctcggatgcc cgcagcagca ggttctcgag agcgaacgcg
     1321 acctcgtcga gcaccgtgcc ggtgacgagc tgcgagtcgg gatcctggaa caccatcgag
     1381 atgtcggtgc tgagctgcgc gaccggagtc gtgcgggtgt cgcgtccgcc gacctcgacg
     1441 gtgccggtca tctcggcctc gaccgactgc gggatgagcc cgttcagcgc gagcgtgagc
     1501 gtggacttgc cggatccgct cggccccatc aggatgacga cctcgcccgc atgcacgtcg
     1561 aacgacacct gctgagggga ggagcgcgcc gcgtcggcgt gcgtgacgct gaggtcgcgc
     1621 acacggagca ggggcgcgga tgagcgcacg tcgggtcctc tgtcggcggc ggatgctgtg
     1681 aacttagcct acccttacct gggtcggcct gccctcggca tccgctgtct cagcgcttcg
     1741 gcgcgatgcc cgctcggcgc agcgcgttgc ccacggcgat gcccacggcg gtccatgcga
     1801 ccggaccgag gaccgagatc gtcaggtaga ccacctgcac ccagaggtcg aagcggccga
     1861 ggtccaccac gaacccgacc acgaccgcca cgatcgcgcc gatgatcacg gcagagacga
     1921 agaagcgcca gcgcccccag gcgcggtacc gggtgagggc ggcgacgccc tcctggatcg
     1981 cgccgaacag cagcgccgtg ccgatgaacc gcatggccca ggcgggattg aaggcgctcg
     2041 agatcagcgc ggcgatcacg tgcgtgatca gggcgaccag tggccggcgc agcacctcct
     2101 gcgcgatgat gccgggcagc acgtgcacgc cgagcaccag accgtacaga tacggggtca
     2161 cggcgaggac gccgagggtg accacacccg cgattccgcc gaggatgccg gtggcgacgc
     2221 cgatggccgc gcagacgagc agtgtgcgcg tgctcaggct ggcggcaggg ggcaccgatc
     2281 caggctacgc gatcggcggg tgacggggcc cgtcgggagt tccacacctg gccgtaagtc
     2341 gctggtgcga tgtccggatg ccgggttacg ttgagacgcg gatgccgcag agcagcggcg
     2401 tccggactca acaggagcaa ctcatgagac gaacacccct cagagtgaca gcggccgtat
     2461 ccctggccgc gttgttcatc ggatccactg ccacctccgg tttcgcggcc gagggggtga
     2521 tgacccccac tcctgtcgac ggaacgccag gccactacat cgtgctcatg aaggcggatc
     2581 cgctggccag ctacgacggc ggcaccgccg gcttcaaggc caccaagcct gccaagggcg
     2641 acaagctcga cgcgcactcc gcgaactcgc agaagtacat cgcgcacctg aagaacgagc
     2701 agcgcaagct cgcggcggag cagggagtga cgccggacgc cgactaccag gtcgtcgcga
     2761 acggcttctc cgcacagctc accggcgcgc aggtcgacaa gctgcgcgcc tcgaaggacg
     2821 tgctcggggt ctacgccgat gaggtgcgcc acccgcaggc ccagacctcc accgacttcc
     2881 tcggcctcgg cgacgacgcc acgggcaagg gcggcgtctg ggagaagacc ggcggagtcg
     2941 gcacggccgg cgagggcatc gtggtcggtg tcgtcgacac cggcatcgct cccgagaacc
     3001 cgtcgttcgc gggggccaag ctgaagaacg ggaacagccc gaagtccaag gggcagccgt
     3061 acaccgacgg caagtacgtg tacttcgaca aggccgacgg caacacgttc aagagcccga
     3121 tcgtcagcgg acaggagtgg gacaagcacg actactccac caagctcgtc ggcgggcagt
     3181 acttctccgc cggcgccaag gccgccggct tcgacttcca gtacgacctg ctgtccccgc
     3241 gcgatggcgc cgggcacggc tcgcacacct ccagcaccgc ggcgggcgac ttcggggtga
     3301 aggcctcggt gtcgggcgtc gacttcggca ccgtctccgg tgtcgctccg gcggccaagg
     3361 tcgccatgta caaggcctgc tacgagggtc ccgacccggc ggtcaccacg gacgacatct
     3421 gcgccctcag cgacctggtg tcggcgatca acgcggccgt gaccgacggc gtcgacgtga
     3481 tcaactactc gatcggcgga ggggccgcca ccggcatcct cgagcccgac gacatcgcgt
     3541 tcttcaacgc ggcggccgcg ggcatcttcg tctcggtcag cgccgggaac gacggccccg
     3601 acccggtcac ggccgaccac gcctcgccct ggtacaccac ggtcgcggca tccaccatcc
     3661 cgacgtggga gggcacggtg aagttccccg gcttcgagaa ggccggcgct tccgtgagcg
     3721 ttccgttcgg atccgaggtc accggcccgt cgatctacgc cggtgatgcg gcagcgggcg
     3781 ccgacgcgaa cctgtgcctg cccggcacgc tcgacgcagc caaggtcaag gaccacatcg
     3841 tggtctgcga tcgcggcagc aatgcgcgcg ccgagaagtc cgaggtcgtc aaggccgccg
     3901 gcggcatcgg catggtcctg gtgaacgtgc ccggcggcgc tgactcgctc gacaacgact
     3961 tccacttggt cccgaccgtg cacctggccg ccgcgaaccg cgcggccgtg ctggcgtacg
     4021 tgcagagcgg caccgacctg cccatcacgc tgatcggcga gaacaccacc ggggtcacca
     4081 ccccggtgcc gcagatcgcc ggcttctcca gccgcggccc gatgaacgcg gccggttccg
     4141 acatcatcaa gcccgatgtc gcggcgcccg gcgtcgccat cctcgccgcg acgaacaacc
     4201 ggcaggacga gcagcccacc ttcaacatcc tctccggaac ctcgatggcg tcgccgcacg
     4261 tcgccggtct cgccgcgctg tacctcggcg agcaccccga cgcgtcgccc gccgagatca
     4321 agtcggcgct gatgaccacc gcctacgaca cggtgaacgc cgacggctcg cgcagcaccg
     4381 acgtgttcgc gcagggtgcc ggtcaggtcg acgcgaagcg gtacctgcag ccgggcctgc
     4441 tgtacctgaa cggtgcgcag gactgggcgg agtacatcga gggcgtcctg ccgggcacct
     4501 tcgacgacgt gaagcccatc gacgcgagcg acctgaacgt ggcgtcgatc gccatcggca
     4561 cgctcgccag cgagcagacg gtcacccgca cggtcaccgc gaccgcgccg ggcatgtaca
     4621 cggccgtcgc atcgatcccg ggcgtgaaag tcaccgtcga gccctcggtg ctcagcttcg
     4681 ccaaggcggg cgagtcgaag agcttcacgg tgacgttcga caactcctcc gccccggtgg
     4741 agaagtgggc gacgggctcg ctgacctgga aggggcagtc cggcgacgtg cgctcgccgc
     4801 tggccgtctt cccggtcacc gccgcagcac cggccgaggt ctcgggcatg ggcgtcgacg
     4861 gctcggcatc cgtgaagatc acctccggcg tgaacggcac gatggcgctg aacgtcaacg
     4921 gcctggcacc gctgcagctg ctcaccgatc cggacaaccc cgtcgacggc cactccggcg
     4981 acggcaagtc cggcgacgag aacaaggacg tcagctggat cgtcgacgtg cccgcgggca
     5041 cgacactgag ccgcttcgac ctggactcgt cggatgacgc gggcagcgac ctcgacctca
     5101 cggtctaccg agtggtctcg cccaccgacc tgcgctacta ccagaagtgg cagtcggcca
     5161 cggcatccgc cgacgagcgg gtgacgatcc cggcgcccac cgcgggcacc tacatggtcg
     5221 tggcgaacat ctactccgca ccgaacccga tgacgtggga catgacctac gccaacgtcg
     5281 ctccgggcgc ggggcagggg gcgttcaccg cgactccgaa cccgctcacc gtcacccgcg
     5341 gcgaggacgt ctcgtacgac ctctcgtgga gcggcctggc ggccgggaag tacctcggcg
     5401 tcgtgcagta cggcgactcc gacgtgcgca ccatcgtgac ggtcgacgcg aagtagcccg
     5461 acacgcgcgg cggggccggt ccttcgggac cggccccgtt cgcatgcgcg gagcggcgtt
     5521 cagccctggt tgggcaggcg gatctcgacg ccgtcgagcg gatgcttggt caggtagctc
     5581 gcgatgaacg ggcagtacgg ggcgatggcg tcgccggtcg tcgcggcatc agcgagtgcc
     5641 tccgcggcga gctgctgggc gagaccccgc ccctggaagg cgtggtcgat ctcggtgtgg
     5701 gtgaagcgga actcgcccgg acgccggtcg aactcggcgt acccggcgag ggtgctatcg
     5761 aggcggatct cgtaacgcga ggcgtcgtcg ttgcgactga cggtgatgtc gggcattgct
     5821 cctccttcgg ttccgtccaa cctacgcgca ccccaccggg aggggcggat gctgtggaga
     5881 gccggatccg aggtcggcgg cggtcgttac gctggatgga tgccgacgcc atccgatccg
     5941 tacgacgaca tcccgtaccc ggatgagcca tggggtgagc cggaggagat ggactgggtg
     6001 ccgccggagg acggctggga gccgccgctg gactgggggt cgccggcaga agcggccccg
     6061 agcccgtcga acgggtcggg cacggcatcc gtgcccaccg cggcgaaggc cgctccctcc
     6121 cggttcggga ccgccctcga ggcgctgcgg acggtgttcg gctacgacgc cttccgcggc
     6181 gaccaggccg cgatcgtcga gcaggttatc ggcggcaggg acgccgtcgt gctgatgccc
     6241 acgggcggcg gcaagtccat cacctaccag gtgcccgcgc tggtgcggga ggggacgggg
     6301 ctggtcatca gcccgctgat cgcgctgatg cacgaccagg tcgacgcgct gcgcgccaac
     6361 ggcgtgcgcg cggcctacct gaactccacg caggcgatcg acgagcggcg tgaggtggag
     6421 cgcgcgtacg tcgccggcga actcgacctc atctatgtcg cccccgagcg gctgtcgtcc
     6481 gcgcagacca cgcagctgct gcagcgcggc acgctcagcg tgatcgcgat cgacgaggcg
     6541 cactgcgtgt cgcagtgggg ccacgacttc cgtcccgact acctcgcgct cggcgatctg
     6601 gccgagcgct tccccggggt gccgcggatg gcgctcaccg ccaccgccac gaaggccacg
     6661 caccaggagc tcaccgagcg gctgcacctg gatcgcgccg cgcacttcgt cgccagcttc
     6721 gaccggccca acatccagta ccggatcgtg ccgaaggtcg acccgcgcaa gcagctggtg
     6781 gagttcatcc gctcacagcc cgagtccgcc gcgggcatcg tctacgccct cagccgcaag
     6841 tcggtcgagc agaccgccga gtacctggca tcccgcggct tcgacgccct gccgtaccac
     6901 gccggcctgc cggcagaggt gcgcgcggcg aaccagtcgc ggttcctgcg cgaggacggc
     6961 gtgatcatgg tcgccacgat cgcgttcggc atgggcatcg acaagcccga tgtgcgcttc
     7021 gtcgcccaca tcgacctgcc caagtccgtg gaggggtact accaggagac cggtcgcgcc
     7081 gggcgcgacg gcgagccctc ggtcgcatgg atggcctacg gcctggggga cgtcgtgcag
     7141 cagcgccgcc tcatcgacca gagcccgggc gatcgcacct tcaagatgcg gatggggcag
     7201 cacctggatg ccatgctcgc cctctgcgag accgtggcct gccgacggca gaacctgctg
     7261 cggtacttcg gtcaggacag tgagccgtgc ggcaactgcg acacctgcct ggagaagccc
     7321 gacaccttcg acggcctcgt gccggcgcag aagctgctgt ccacgatcgt gcggctcaag
     7381 cgcgagcgca accaggcctt cggcgcgggc cacctgatcg acatcctgcg cggctcctcg
     7441 aacgaccgca tccgcaagat gcgccacgag cagatcgcca cctacggcat cggcgccgac
     7501 ctgtccgacc aggactggcg cagcgtcgtg cggcagctgc tggcccgtgg catcctggtc
     7561 gcgcagggcg agtacggcac cctcgcaccg ggggacgccg ccgggggagt gctgcggggc
     7621 gacaccccgg tgccgctgcg caaggacacg atcgggcgcg tgaccgcgtc ccgcacccgc
     7681 aagacgagtg ccgcggatgc cctggatgcc gaggatcagc cgctcttcga ggcactgcgc
     7741 acctggcgtg cggccaccgc gcgcgagcag ggggtgcccg cctacatcgt gttcggcgac
     7801 gcgaccctgc gcgctctcgc cgagcatcgg ccggcgtccg tcgacgcgct cagcggcatc
     7861 agcggcatcg gcgagaagaa gcgcgacgcc tacggcgagg ccgtgctcga ggtgatcgcg
     7921 gcgcactgac gccggagcag gaggaaaatg ccggtctcgg ctgtttcagg ccgacgttct
     7981 ccttctttcg caatggatcg aagccgttca gcgcgcgcca cgtggacgcc cgtgaggagc
     8041 agcttcacgc cgccgcgaac tcctccgtgt gcacgtgcac ggtcgcggcg ccgccgatca
     8101 gcttcgacac cgggcagcgc gcgtgtgcgc gctcgagcag gtcctgcgcc tcggcatccg
     8161 tcatcccctc cgccgacagg aacgcgtcga cgtggaaggc gtatccgggc ccgggcgcga
     8221 ccggatgcag ttcgacctcg acgcggacgt gggtgcgccg ctgctcccgc accagcacct
     8281 gggcggtgcc gttcaggcag gtcgcccagg ccagggcgag cagctgctcg gggttcgtgg
     8341 ctgcgggatc ggcatccgcc ttcagcggcg aggcgaccgc gacgtccagc ccatccggca
     8401 cccggctccg cccgtcgacg ccgttgctcg cttccgtccg atacctggca ctcaccattt
     8461 cagcctaggt gtgggcggac cgagtcggct cctggttgtg acgtttcatg acgccccgat
     8521 gcggaaccct gtcatgacag gcaatagcct ctcagtccat gagcgacctg cgcaccacga
     8581 cgcccaccag catccgcacc gcacgcgatg tgcacgccct gctcgccgag aagggttcgc
     8641 agggtgcgcg gaccaaggcg atcatcgtgc tggcgctggg cggcatcttc atggacgcct
     8701 atgacttctc gtcgctggcc ttcggcatca cggccgtcaa ggaggagttc ggcctcgacc
     8761 cgctgatgac cggggtcgtc aacgcggcga tcatggtcgg ctccgtcgtc ggcgccatct
     8821 tcggcgggat gctggtggac cgcttcgggc gctacaagct gttcatggcc gacatggcct
     8881 tcttcgtcgt ggcggccatc ggatgcgccc tcgcgccgaa cgagtgggtg ctcatcctct
     8941 tccggttcgt gatgggcatc ggcgtgggtc tcgacctccc ggtcgccatg gccttcctcg
     9001 ccgagttctc caagctgcgc ggctcgggca gtcgatccca gcgcgtgaac gcctggtcgc
     9061 cggcgtggta catcgccacc ggcttcggct accttctggt gctcgcggtc ttcctgcttc
     9121 tgcctgtggc gcagcacggc atcctgtggc gagtcgtcgt cgggttcggg gcggtgccgg
     9181 cgatcatcgt gctgctcgtc cggcgcaagt acatggccga gtcgccgcag tggctcgccg
     9241 atcagggcga cctgcgtggc gccgtcgacg tgatgcgctc gcaccacggc ctcgacgtcg
     9301 agctggcgcc ggatgccgac accgcccgca ccacgaaggc gcgcaccggt cactggcgtc
     9361 agtacggcga gctgttcgcc gcccgctacc gcctgcgcac catcgcggcg ctgtgcgtgt
     9421 cggtgttctc caccttcggc tacaacgccg tcgcctacgg aacgccgctg atcatcgcga
     9481 tgctcttcca gcagggtccg ctcgtgacca tcctgtccgc gctcgtcatc aacctcggct
     9541 tcggcgccgc gggcggcctg ctcggcgtcg gcctgatcaa ccgggtcggc gcacgccgga
     9601 tgacgatcat cggcttcgcg atccaggcgg cctccctgct gatcctcgcg ttcgtcggca
     9661 tcccgaccgg cgcgctggtg atcatctcga tcgcgatgct cgcggcgttc gtcttcgcgc
     9721 aggccggggg tcctggtgcc aacctgatga gctacgcgac cctgtcgtac cccacgcgcc
     9781 tgcgcggggt cggcgtcggc ttcaacgagg cggtcaagcg ggtcttctcg atcgtgtcgc
     9841 tcgtgttctt cccggtgctc gccgcggcac tctccaccgg cgtgttctgg atcgtcgccc
     9901 tcgcgcccct cgccggcctg atctcgctgc tcgtgatccg ctgggacccc acgggcaagg
     9961 acgtggacgc cgaggaccag gaggcctgat ctgcggcgat cccggagcgg atgctgtggg
    10021 gtgacgacaa cgcggacgga gaccggggcg cggcctgctt ggtgaacatc cacaggcgag
    10081 ttccccggaa gttgtcgcag acctaccatt gaggcatccc tgctccctct tgagaggaat
    10141 cgccatggtc gacgccgccg tccgccccac tgccgcatcc gcccccgaag ccggcatcga
    10201 tgtcgcgcgc gtgaacgagc tgctcatggg cacgtgggcg cacacgcgcc gcgaggcgcg
    10261 cgagctgatc aaggatccgg cgttctggcg cgacgacagc ctcggcaagg acgagcatcg
    10321 cgagcgcgtg ctcacgcagc tgcatctgct ggtcgagaac aacgccgtgc accgcgcctt
    10381 ccccaaggcg ttcggcggcg aggaggacaa cggcgccaac atcgccggct tcgaggagct
    10441 cgtcgcggcc gatccgagcc tgcagatcaa gtcgggcgtg cagtggggac tgttcggcgg
    10501 cgcggtgctg cagctgggca ccgaggagca tcacaagcgc tggctcccgg ggatcatgga
    10561 cctctccatt cccggcgcgt tcgcgatgac cgagatcggg cacggctccg atgtcgcggc
    10621 cgtcggcacc accgcgacct acgacccgca gaccgaggag ttcgtcatca acacgccgtt
    10681 ccgcgcggcg accaaggagt acctcggcaa cgcggcgctg cacggcatcg cggcgacggt
    10741 gttcgcgcag ctgatcacga acggcgtgaa ccacggcgtg cactgcttct acgtcccgct
    10801 tcgcggcgag gacggcgagg acctccccgg catcggccgc gaggacgacg ggctcaaggg
    10861 tggactgaac ggcatcgaca acggccggct cagcttcgac cacgtgcggg tgccgcgcac
    10921 caacctgctc aacaagtacg gcgacgtcgc ggccgacgga acctacacga gccccatcga
    10981 cagccccggc cgccgcttct tcaccatgct cggcaccctg gtgcaggggc gcgtctcgct
    11041 cgacggcgcc tcgtcctggg catcggctct cggcctgcac atcgcgatca cctacgcgac
    11101 ccagcgccgc cagttcgacg gcgcggacgg gcaggagacg gtcctgatgg actacggcaa
    11161 gcatcagcgc cgtctgctgc cgcgtctggc gaccacgtac gcgcagatct tcgcccacga
    11221 cgagttcctg cagaagttcg acggtgtctt ctcggggcgc accgacaccc ccgaggaccg
    11281 tgaggacctc gagacgctgg ccgccgcgct caagcctctc tcgacctggc acgccctcga
    11341 caccctgcag gaggcccgcg aggcctgcgg cggtgcgggc ttcatgttcg agaaccgcct
    11401 ggtcggactg cgcgccgacc tggacatcta cgccacgttc gagggcgaca acaacatcct
    11461 gctgcagctg gtcggcaagc gcctgctcac cgactacgcc aggcagttca aggccaagga
    11521 cgccgccgcg ctggccaaat acgcggtcgg gcagaccgcc gaccgggtgt tccacggtgc
    11581 ggggctgcgc gccctcggcc aggccgtcgc cgacctgggc tccacggcac gctcggtgga
    11641 gaacggcctg cgcgccgagc agcagcacga gctgctcacc gagcgcgtgc agcagatggt
    11701 cgccgacatc gccggccggc ttcgtcccgc gggcaaggac agggccctcg gcgagaagct
    11761 gttcaaccag aaccaggccg agctcatcga agccgcccgc gctcacggtg agctgctgca
    11821 gtgggaggcg ttcaccgacg cggtcaacgc gatcgaggac gaggacacca ggcaggtcat
    11881 gacctggctg cgcgacctgt tcggcctgac cctgatcgag aagcacctgg cctggcacct
    11941 gatcaacggc cgcctgtcca cgcagcgtgc ggccgccgtc tcgagctaca tcggccggct
    12001 gtgccaccgc ctgcgcccgc acgccctcga gctggtcggc gccttcggct tcgagcccga
    12061 gcacgtgcgc gccccgatcg cgagcggcat cgagcagcag cgccaggacg aggcccgcgc
    12121 gcactacgcc gcgctcgccg cctcgggtga ggcgccgatc tcggagaagg aactgcgcaa
    12181 gcaggccaag cagaagaacg gctgagtcgc ggggcatccg cagcacacct gcacgacccg
    12241 gaaacacctg cacgaccgat tcgccgatcc ggccgtgcat ccgttcccgg gtcgtgcatc
    12301 cgtatccggc tcggggcccg cggatgctga ccgtccgacc cccgcgatag cctgaacgca
    12361 tgagcaacgt gacgcagatc ggcgaactcg catcctgggg cggcgagaag ggcaagcagt
    12421 tcctggacaa ggagatcggc acggagttcg ccggcgtctc ggtgaacacc accgccccgg
    12481 gcagcgagtc ccccttctgg cacgcgcacg cgaagatcga ggagatctac atcgtcgtgg
    12541 acggtcgcgg cgagttcgcg ctggatgacg aggtgctccc gctcgaggcc ggcaccgcgg
    12601 tgcgcgtggc tcccggcgtc atgcacgcgc tgcgcgccct gcccgagagc ccttcgccga
    12661 tgcgctggct gtgcatccgt gcggcgggcc aggagctcgc ttcggtcggc cacgacgccg
    12721 agctcgaccg cgagcgtccg ttcccctgga atgcctgacg catggcgtcg gtcctgaccg
    12781 gtcccgacgg ccgcggccgt tgcgcgtggg tcggtgacga cgccgagtac cgccgctacc
    12841 acgacgagga gtggggcacc gcgctgcacg gtgaccgcgc gctgttcgag aagatgtcgc
    12901 tcgagggctt ccaggcgggg ctcagctgga tcacgatcct gcgcaagcgc ccgcgcttcc
    12961 gcgaggtgtt cgccggcttc gatcccgcac tggtcgcgga gttcggcgag gaggacgtgg
    13021 agcgactgat ggcggatgcc gggatcatcc gcaaccgcgc caagatcctc gcgacgatct
    13081 ccaacgccgc gctcgtgcgc gagatggccg acggcgagct ggatgctctg atgtggtcgt
    13141 tcgcgcccgc ggcatccccc cgtccccgca ccttcgacga cgttcccgcc gtcacggccg
    13201 agtcgaccgc gctcagcaag gcgctgagga agcgcggctt ccgcttcgtc ggtcccacga
    13261 ccatgtacgc gctcatgcag tccgccggga tggtcgacga ccacgtcgac ggatgctggc
    13321 gggcctcagg tcgctgaccc cggccggagc cgccggcatc cacgtcggca tggtcacttc
    13381 tccccatggc cccgtcgtag ccccggggta aggtgggcgt ggcgcgctgt atgcgcgttc
    13441 aaggggggaa catcagtatg agggtactga gctggatgcg cgcgcgcccg aaaagattgg
    13501 ctgccggagc cggggtcgcg atcgccgcgg tggcgctgac cggcatggcc gtggcctatg
    13561 acggcctgcc gacgacgaag gtcgatctga acgacgccgg cgtctggctg accaagtcgt
    13621 cgagcctgct ggtggggcac ttcaaccacg agtcgaccgt cctcgacggc gggctgcgca
    13681 cctcgagcga gagctatgac gtcctgcagg accaggcctc ggtgctgatc gtcgacgacg
    13741 gcagtggaac gatgtccccc gtcgatcccg cacgggtctc cctctccggc gcggtcaagc
    13801 tgcccggtga cgcccaggtc gcgctgaggc ggaacacggt cgcgatcatg gagcgctctt
    13861 caggtcgcct ctgggtgatg ccgttctccg gcgtcgcgaa cttcgagccg ggcagcgctg
    13921 agcccaccgc cactctcggc aagaacgccg cggtcactgt cggtgccgat ggcacggtgt
    13981 acggcgtcgc aggcgggaag ggcgagatcc tcaccgtcac cacggatgca cagggcctgc
    14041 cgctggagga gaagcgcgag tccgtcggca agatcgacgg tgccgcgcgg ccgacggtga
    14101 cggcggtcgg ctcgaccccc gtcgtgctgg actcctccgg caccgtgatc gcgcccggtg
    14161 gtgtgcgcgc cgaggtcgcg tccgacgggt cggcagtgct gcagcaggcg tccaccgaga
    14221 cgggtgccgt cacggtcgcc acctcgaacg ccctggtgcg cgtgccgctg gacggcgccg
    14281 agcccactcg caccacggtt cccagcaccg gaaacccggc cgcgcccgtg ttcctggacg
    14341 gctgcacgta cggcgcatgg gcgggctccg cgcgcttcat ccgtgactgc gccggcaaga
    14401 gctccgatgt ggcgacgaag gtcccgggcg ccgaggcgtc cacccagctc accttccgcg
    14461 tgaaccgcga tgtgatcgtg ctgaacgaca tcatcggcgg cgccgcctgg cttgccgacg
    14521 agagcatgca gcgggtggat gactggagca tcctcacccc tcccgagggc gaggcggagg
    14581 acgaggagaa gaccaccgag gagaccgtcg agacgaccct cccggagcgc aaggaggaga
    14641 acaccccgcc gaccgccgag gacgaccccg ggctgggcgt gcgccccggt tccaccacca
    14701 tgctgtcggt gctggacaat gacaacgacc ccgacggcga cgtcctggtg gcgtcgctgg
    14761 tcggctccca gccctccgtc ggcgaggtgc agccggtgct cgacggcgct gcgctgcaga
    14821 tcgccgtgcc ggaggacgcc acgggcacgg catccttcca gtaccaggcc gacgatggcc
    14881 ggagcgggac cgacaccgcg acgacgaccg tctccgtgca cgcctggagc gagaactccg
    14941 cgcccaagac gaagcgcgtc accaagctcg ccgtggagtc cggcggcacg atctcgtaca
    15001 acgtccttcc cgactggatc gaccccgacg gggatgacat ctacctgcgc gaggtcgtcg
    15061 ccgctcccgg cgacgaggtc gagttcacga ccggcggaca gatcacctac cgcgccgtgg
    15121 gcagcctgca gggccgcaag gaggtgcagg tcaccgtctc cgacgggctc ggcgaagagg
    15181 cgaacggcac catcctgctt gacgtgcagc cgccggggtc gacgctgccg aagacgaacg
    15241 ccgaccacgt ggtgacccgt cccggagtcg ccgtcacggt atcgcccctg accaacgacg
    15301 ccagctcggg ccgggagcag ctgcgcctcg cgggtgtcga cgacgtcgac ggcgccgtga
    15361 tctcgtcgga cctcccgaac aagcagttca ccttcacgtc gcagacggcc ggggtctact
    15421 acgtgcagta cctggtcgcc gccgggccca atgcggtgcc cggcctggtg cgcgtggatg
    15481 tgctggacac cgccgacagc gagctgccgc ccgtcgcggt gcgcgatgtg gcgctcctgc
    15541 ctgcgggcgg cgaggtcctg ctggacccgc tggccaatga ctccgatccg tcgggcggcg
    15601 tgctggtgct gcagtccgcg agcctcgaga cggactcccg gggcatctcc gtctcggtcc
    15661 tcgagcatca gaccctgcgc atcggcgacc agggcggact gagcgggccc gccaggatca
    15721 cctaccgcat ctccaacggc acccgctcgg cggagggtga ggtcgtcgtg ctgccgatcc
    15781 cggccccgga tcggatcctc gcgccggtgg ccgtcgacga tcaggccgtg gtgcgcgccg
    15841 gcgacgtcgt gacgatcccg gtgctcgcga acgactcgca cccgagcggt gacgcgatcc
    15901 atgtggtccc ggcactcgtg gagcccctcg tcgatccggc cgacggcgag atcttcgtgt
    15961 cgcaggacac ggtgcgcttc cgcgccggac ccgagccgaa gacggtgaac gccacctacg
    16021 agatcatcga ctcgatgggc cagaaggctg cggcctacat ccgcatccag atcgtcccga
    16081 tcgacgcgga gaagaacgca gcgcccgtgc cgcgcgacct cacggtgcgc acgctcagcg
    16141 gggcgacgca gcgcatcgcg atcccgctgg acggcctcga cgcggacggc gactccgtgg
    16201 agctgctggg gctcgcctcc agtccgcaga agggccgggt ggtcgagacc gggcaggact
    16261 tcctcagtta cgaggcgttc gacgacgcga ccggcgtgga cagcttcacc taccgggtgc
    16321 gcgatcgact cggccgcgag gggaccggca ccatccgggt cggcatcgcc ccgcgcgaga
    16381 ccgtgaacca ggcgccttac gccgtgaagg actccgtggt cgtgcgcccc gaccgcgagg
    16441 tcgccgtgcc ggtgctgatc aatgactccg accccgaggg cgggaagatc tcgctggtga
    16501 agaacggcgt ctcccagccg gatgtggacg gactgaaggc ccgtgtgtcc ggcgaccgcc
    16561 tgctgatccg cgtgccggat cgcgaactgg agacctcgct gcgctacacg atcgtcgacg
    16621 agctcggcgc cacggcgacg gccgtcgtcc aggtcaccgt cgacgaggac gtgccgctga
    16681 aggctccgat cgcacgcgac gacagggtgc tggtgacgga cgtcaaggac agcgccacga
    16741 tcgacctgga ggtgctcgcc aacgacgagg accccgacgg cacgacgcag gccctctccc
    16801 tcgaggcggg cgcgggcgga acagtgcgcc ccgaaggcaa ggtgcgcgtc accgtgggcg
    16861 agaaggcgca gatcgtgcgt tacaccgtca ccgatcggga cgagttgcag gcatccgcct
    16921 tcatctacgt ccccgcgctg tccgacatgc gcccggtgct ctcgcgcgtc gagcccgtca
    16981 cggtcaagag cggcgagacg aaggtgctgc cgctgaagga ctacgtgacg gtcttcggag
    17041 gcggccgcgc catcatcacc gaggcggcga aggtcgtcgc accgcacagc aacggcgcca
    17101 acctcatcca ggacgagagc accctggtgt acacctcggc cgagggctac ttcggcaagg
    17161 attcgctgac cttcgaggtc acagacggga ccagggtcga cgacccgaag gggcggaagg
    17221 ccaccctgac gatcccgatc accgtgcttc cgcccgacaa ccagccgccg accttcaccg
    17281 acgcccagat gcaggtcgct cccggcgagg atgcgacgat cctggacctg gccgccctgg
    17341 cctccgaccc cgacccgggc gacaaggtgt cgttccgtct gacgggaggg gcgggcgagg
    17401 gcatcaccgc ccggatcgac ggcacgaaac tgagcgtcga ggcggcgaag gacgtgcgca
    17461 agggcacggt cgcgaacctg gagatcactg ccacagacgg caccaccgag cccgtgatcg
    17521 gacgggtcgt cgtcaccgtg acggcatcca cccgcgacct cgcgatcgcg acggacgatg
    17581 tgctcgacga ggtggatcag ggcaagccgc tcaccgtccc ggtgctcgag aacgacatca
    17641 acccgttccc ggacactccg ctgaagatgg tctccgcatc gctggagacc ggtcgtggcg
    17701 aggtcacgac gaagggcgac caggtcgtcg tcaccccggc cgcggacttc gtcggggtgc
    17761 tcgtcgtgcg ctaccgcatc caggatgcga cgaaggaccc cgaccgcgag gtcgacggtc
    17821 gcgtccggct caccgtgcag ggagtgccgg atgctcctgg gacgccgcgg gtcaccagcg
    17881 tccaggaccg caccgtggtc ctgtcatggg cgtcgccggt gaacaacggc gccgagatca
    17941 cgaagtacac ggtccgctcc gtgcagggcg gtgcgtattc gaaggactgc gaatcgacga
    18001 cctgcacgct ggacaggctc accaacaacg tcgagtacgt gttccaggtc accgcgacca
    18061 accgggtcgg agaatccaag ccctcgccct ccagcgaggt ggcgcgtccc gacacccgtc
    18121 ccgatgcgcc cagtgcaccg cggctggcgt tcggcgacca cgaactgaag gtcgcgtgga
    18181 ccacgccgag cacgtcggga tcgccggtcg agtcgtacac gctgcagatc agccccgctc
    18241 cgccctccgg gcccgcggag atcagcggcg tcaccggcac cagcactgtg tggaaggggc
    18301 tcgagaacgg gcgcgcctac caggtgagga tccgcgcgca caaccgtgcg ccggagccgt
    18361 ccgattggag cctctactcg ctcgccgaga ccccggccgg tccgccgatg gcgcccgcgc
    18421 agccgaccac ggcggagctg gatccggtgg gatcgaaggc gcagatccag gtgaactggg
    18481 tcgcgccgga cagcaacggc gacaagatct ccggttacga gctgcaggtc ctgcagggca
    18541 gcacagtggt caggaccctc aagccggccg cgggggcgac ctcgcaggcc gtgcaggtcg
    18601 acccctcgga gacgggctac acgttccgca tccgcgcgct caacaaggct ccgctgccgg
    18661 gcgagtggag tccgccctcg gccgagcgtc gcggcgtgct cgccccgggc gcacccacga
    18721 gcgtgaaggc gactcccggt gacaacaccg tcacagtggc ctacacaccg gggccgctca
    18781 acggcgcgaa ggcgagtgag gtcacgtacc agtaccgcgt cggtgcgggc ggctcgtgga
    18841 atgccatgcc cggcgaccgg gtgatccgca gtggcgtgaa caacaacggc acgtatcaga
    18901 tctacgtgcg ggcgatctcg aacgtcgcgg ggaccacctc gaaggcaggg cccgcgtccg
    18961 cggcctcgaa tgatgtggcc ccctacggga ggccgaacgt gccgagcctg tcagccagcg
    19021 tgagcgggca gacgatcacc tggcacatcg gcgcgaccgc acgcaacggg cgcgatttca
    19081 ccgtgacctg ggccgacaac cgcgggcatt ccggcagcat cggtgcaggc ggcggcaacg
    19141 tgccgctgtc gtacgactgg agcaccccgg tgtccttcac cgtgacggtg acggacaagc
    19201 catccgcctt gcagcctgcc ggcgcgcaga cgaacgccgt caccgggaac agcggcaccg
    19261 gacccaagcc gcttcccgag gtatgggtca gtcgcggagg tccggcgacc tgggggccga
    19321 gcacgtgcca gaacgtgcgc gtgaccgtct ccagcgactt cccggcgggc aggtacgtcg
    19381 tggatgcata caacaacggc gtctggttcg ggaacaacaa gggcaatgcg gtgcaggtcc
    19441 ccggagacct gggatcctac gtgtgccccg gcgaggcggg tcacacgatc tcggtgagca
    19501 tccagggagt gaacgcgacc gtccatccgg tcatctggtg agtcccgcag ggtgatcggg
    19561 aggggcagcg ctccctcccg atcgtcgttc tcgggcggcg tcgtctagga tgaagccgcg
    19621 cgttcgggat gacgcgcggc aactgacatt gtgcggggga atgccggtga gagtcctgtc
    19681 gtgggtgcgc gcacgtccga agacgcttgc ggcgactgcg ggcgtgctgg ccggcacggt
    19741 ggtgctgacc ggcatggcgc tggcctatga cgggcagccg accaccaagg tcgatctgaa
    19801 cgacgcgggc gtctggctga ccaagacctc gagcctgctg gtggggcact tcaaccacga
    19861 gtcgacggtg ctcgacggcg gtctgcgcac cagcggcgag aactacgaca tcctccaggc
    19921 cggtgcgacg gtgctggtcg ccgaccaggg gaactccacg ctcacggcga tcgacccggc
    19981 caatgtcgcg ctcaccgaca gcgccgcgat cccctccgac gcgaaggtcg ctctgggctc
    20041 gcacacggcg gcggtgctgg accgcaagtc cggtgacctc tgggtggtgg gcgcctcatc
    20101 ggtgtcgggt ttcgacgtga agggcaccaa gccactcgcg gagctcggca aggacgccga
    20161 cgtagctgtc gcgcaggacg gcacggtgtt cggcctgtct gtcgagcgcg gcgagatcgt
    20221 gacgatcccc gtcgaccggc agggcgaccc gctggccgcc agcacggcat ccgtcggcga
    20281 gatcggagcc tcgccgcgtc cctcgatcac ggcggtcgga tcgacgccgg tggtcctcga
    20341 cgccgcggag ggcgtggtgt ccacgcccgg cggcatccgg gtcgacctcg ccgacctcgg
    20401 ggccgggtca ctgaaggatg ctgtcctgca gcaggcatcg gcgcccgcgg atgccgtggc
    20461 cctggccacc gggcagacgc ttgtcgagat cccgctggac ggggcgaagc ccgtcgtgcg
    20521 gaaggccggc ggcacgggga tccctgccgc gccggtgttc ctgcaggggt gcacctacgg
    20581 cgcctggggc ggctcgggcc gtttcctgcg cgactgcgcg ggagacgcgg acgacgtggc
    20641 cgccgacatc ccgggcgcgg agcagagcac gcacctgacc ttccgcgtga accgcgacgt
    20701 gatcgtgctc aacgacgtcg tgggcgggga ggcctggctc gcggacgaca gcctgcagaa
    20761 ggtcgacgac tggagcatcc tgaccccgcc cgagggtgac gccgaggacg aggagaagac
    20821 caccgaggag acggtggaga cctcgctgcc cgagcgcaag cccgagaaca cgccccccgt
    20881 ggcggaggac gaccctgatc tgggtgcgcg tcccggcgga accaccctgc tgccggtgct
    20941 ggacaacgac aacgaccccg acggggatgt gctggtcgcc acgctcgacg acacccagcc
    21001 tcaggtcggc gaggtgcagc cgatcctcaa cggcgcagcg ctgcagatcc tggtgcccga
    21061 cgacgcgaag ggcacggcga cgttccgcta caaggtggac gacgggcgcc cttcaggtac
    21121 cgacaccgcg aacgccacgg tcacggtcta cccctgggac gtcaacacgg cgcccaagcc
    21181 caagcgcaag gccaagctgt ccgtcgagtc gggcggcacg ctctcgtacg gcgtactcgc
    21241 cgactggatc gaccccgagg gcgacgacct gtacctgctg aacgtgacgg cggcgcccgg
    21301 cgacgaggtg gacttcacca ccgacggcca gatgacctac cgcgcggtgg gcagcctgca
    21361 gggtcgcaag gagatcgagg tcgtggtggc ggatgctctg ggcgagaccg ccacgggcaa
    21421 gatcaccctg gacgtcaagc ccgccgggac caccgttccg gtgacgaacg ccgaccacgt
    21481 cgtgacccgc gtgggcgaga ccgccacggt gtccccgacc gccaacgaca cgagcacggg
    21541 tcgtgacgag ctgcggctca cccgcgtcga gccgctggac ggcgtgcaga tcgtgcccga
    21601 ctatccgaag aagcagttcg ggttcaaggc atccgctcct ggcgtctact acgtgcagta
    21661 tctggtctcg gccgggcagg cggacgccaa gggcatcgtg cgcgtcgacg tgctggacga
    21721 gaaggcatcc gagctgccgc cggtcgctgt gcgtgacgtc gcgctgctgc ccaagggcaa
    21781 ggaggcgctc gtcgatgtgc tcgcgaacga ctccgatccc gccggcggcg tgctggtcgt
    21841 gcagtccgtc acggtgccgc cgcacagcgg catcgacgtc tccgtgctga accacagcac
    21901 acttcgcatc ggcgaccagg gcgccctcga ccagcccgtg aagatccggt acacgatctc
    21961 gaacggctcc aagtcggcgc agggcgacgt caccgtgatc ccgatcccgg cacccacgaa
    22021 actcgccgcc cccgtcacgc ttgatgacga agcggtggtc cgcgtcggcg acgtggtcac
    22081 gatcccggtg ctggacaacg acgtgcatcc gaacggcgac gtgctgcatg tcgcgccgga
    22141 cctgatcgag ccgctggtcg acccgaagga cggcgaggcg ttcgtctcgc aggactcggt
    22201 gcggttccgg gcgggcagcg agccccgcac cgtctacctg acctacgagg ccgtcgattc
    22261 gatgaatcag aaggccggcg gacacgtcac cattcagatc ctccccgtgg acgaggaggc
    22321 caacgcggcc ccgcggccgc gcgatctgac cgcacgggcg ctgtccggcg gcagcatccg
    22381 catcccgatc ccgctcgacg ggatcgatgc cgatggcgac tcggtcgagc tgctcggtca
    22441 ggacgtcgcg cccgtcaagg gccgcatctc cgagaccggc agcaacttcc tggagtacca
    22501 ggccttcgcc gacgcgacgg gagtggacac cttcacctac cgcgtgcgcg accgtctcgg
    22561 cgccgagggc acggccacgg tccgcatcgg catcgcgccg ccggagtcga cgaaccagtc
    22621 gccgtacgcg gtgaaggacg ccgtcatcgt gcgccccggc cgctccgtcg ccgtccccgc
    22681 gctcgccaat gactccgatc cggatggcga cgagatccgc ctcgtgaagg acggcatcga
    22741 ggtgccggcg gacaccggca tcaccgccaa ggcatccggc gaccgcgtgg tcgtgaaggt
    22801 gcccgaccat gagctcgagg catcgctgca gtacacgatc cgcgacgcga ggggagcgga
    22861 ggcgaccgct cccatcctgg tgaaggtcga caagaacgtc cccctgatga cgccgatcgc
    22921 ccgcgacgac cgcgtgcgag tcgaagacct gaaggactcc ctcaccacgg acgtcgacat
    22981 cctcggcaat gacgaggacc cggacggcac ggtcgagggg ctgaaggtcg cggtcgagga
    23041 cggcgggcgg ctgctgggcg accgcaaggt gcgcgtgacg gtcaccgaca agcgtcagct
    23101 ggtgcgttac acggtcaccg accaggacgg tctgacggcg tccgcgttca tcttcgtccc
    23161 ctccatcagc gagctgcgcc ccgtgctgcg ttcgaccaag ccggtcgagg tcaagagcgg
    23221 cgagacgaag gagctcaagc tctccgagta cgtgatggtc gcgggcggag gcgcggtgcg
    23281 cctgaccgag gcgtcgaaga tcagcgccgg ccacggcaac ggcgactcgc tcatcaagga
    23341 cgagaccacg ctcgtctaca cctcgaagaa gggctacttc ggcgacgacg cggtgagctt
    23401 cgaggtcacc gacggcaaga ccgtcgaaga ccctgccggc cgcaagagca ccctgtccat
    23461 cccgatcacc gtgctgccgc ccgagaacca gccgccggtg ttcgtgaacg ggcaggtgga
    23521 cgtcgccccc ggcgagaagg cgacgcccct ggatctcgcc gcgctcacga cggatcccga
    23581 caaggcggat gccgacagga tccagtaccg catcaccggt gaccccggcg agggcgtgtc
    23641 cgcacgactg gacggcaaga gactgctggt cgaggcgggc acgtcgacgc cgaaggggac
    23701 cagcacctcg gtcaggctga gcatcaccga cggcacgacc gaggcgatcg aggggacggt
    23761 cgcggtgcgc gtgatcgcat cgacccgcag ccttccggtg gcgagccctg acacgatcga
    23821 tgaggccgat cagggcaagt cctacaggat ccccgtgctg gagaacgaca tcaacccgtt
    23881 cgccgatgag ggcactccgc tcaaggtgct ctccgcgacg gtggagagcg gcgacggcgg
    23941 cgcgaacgtc gtgggcgacc aggtcgaggt gacccccggc aagaagttcg tgggcgtgat
    24001 ggtggtgcgc taccgcatcc aggatgcgac gaaggacccc gaccgcgagg tcgacggccg
    24061 catcacgatg acggtgcagg gcgtgccgga tgctccgggt gtgcctcgcg tgaccacggt
    24121 cgaggaccgc acggtggtgc tgaactggtc ggcaccgtcg aacaacggcg ccgagatcga
    24181 ccactacacg gtcacctccg tgggcggccg cccctacacg aagacctgcg agtcgacgac
    24241 gtgcaccctg gacggcctga ccaacaatgt cgagtacacg ttcaaggtca ccgcgcacaa
    24301 ccgcgtcggt gacgggccgg agtctccggt gtccgaaccc gcgcgccccg acgtgcgccc
    24361 cgagcggccg ctgccgccga cgctggtgtt cggcgaccag tcgctgaagg tgtcctggac
    24421 cacgccgcag tccaatggct cgccggtcga ctcctacacg ctgcagatct ccccggcgcc
    24481 gctgcgcgga gcatccgaga agaccggtgt gaccgggaac agcatcgtct gggacgggct
    24541 ggagaacggc accagctatc aggtgcgggt gcaggcgcac aaccaggcac cggatccgtc
    24601 cgacttcagc gtctattcgc agcccgagat cccggccgga aagcccggca cggtcgcggc
    24661 tccgacggtg agcagcgcgc cctcggtggg cagcgaggcg cagatgacgg tgcgctgggc
    24721 cgatgccgac ccgaacggtg atgcggtcaa gaactacgac ctgctggtct accgcggcgg
    24781 ttcgctgttc cagaccctgg ccgtgggcac ggcgacgtcg aagacggtct cggttccgac
    24841 gaactcgtcc gactacacgt acgcggtgaa ggcgacgaac aaggcgggta ccagcgcgct
    24901 gagcccgcag tccactccgc agcgcgcgtt cggcacgccg ggggcgccga cgaacgtcgt
    24961 cgcaaaggaa ggcgacacgc agatcaccgt caccttcacc aagcccgact tcaacggcgc
    25021 caaggacagc gagatcaggt atcagtactc gctgaacagc ggtacctgga cgaactggaa
    25081 cggcacgagc gcgattccgg cgaacaacgg aaccgcctac accgtcaagt tgcgcgcata
    25141 ctcggtggtc ggcagccagc agtcccagcc gggcaaggag tccgccccct cgaatcaggt
    25201 gaccccgtac ggggcacccc atgcgccgac cggcagcgcg acgaacctcg gccaggcgat
    25261 ccggctcgcc tggaacgcaa gcggctccga caacggacgt cccgtgacca catacatcag
    25321 catcgacggc ggcggttggc agcaggtcgc gatctcgggc agcactgacg tcggcaacgg
    25381 ctacagccag acgcacagca tccgggtgcg cgcggtcgcg aaccccggtg gtgaggcgct
    25441 gtccccggtg tactcggcca cgaccaatcc gccgcctcag ccgtccgcgc agaccgtgcg
    25501 aggaaccctg gcggtcgatc acggctggtt gaacggctac agctgcgcgc aggacacctg
    25561 ctactacgtc gcggcggaca ccgcgaactt ccccgccggc aactatcgcg tcacctgcta
    25621 tgcgaacgga tccgccttca gctcgtcgta cagcacgcag tacgtcccgg ccaacggccg
    25681 ggtccaggcg acgtgctgga cgggcggcaa ctccggtggc gactacaaca tccagctcta
    25741 catcgagggc tggggactcg ccgcgccgac gaactggcaa tgacggcccg cggcggtggc
    25801 cgcggggacg ggtagtactc cccatccgct cgcctgggcg gcgtgtcgcg cgacacgtaa
    25861 gctgacacgg gcgccggaag gcgcgccgag ggagagctac cggtatgaga ctgatgacgt
    25921 gggtgcgtgc gcgtccgaag acccttgccg ccaccgccgg cgtgctcgtc ggcagtgtgg
    25981 tcctcacggg catggccctc gcctatgacg gcctccccac ggccaaggtc gagctcaacg
    26041 acgcgggcgt ctggctgacc aagtcctcga aactgctcgt cggccacttc aaccatgagt
    26101 ccacggtgct cgacggcggc ctgcgcacga gcagcgagaa ctacgacatc ctccagaacg
    26161 gcccgactgt actggtcgtc gatcacggca actccacgct gaccgcggtc gaccccgcca
    26221 acgtctccct caccgacagc gcgacgattc ccggagacgc gaaggtcgcg ctcggcgacc
    26281 gcaccgcggc cgtgctggat cgcgcatccg ggtcgctctg ggtgctcggc gcgagcgaga
    26341 tcgccggctt cgacatcaag ggcgcggacc cggtcgccaa actcggcgcg gacgccgacg
    26401 tcaccgtcgc gcacgacggc accgtgttcg cggtgtcccc gaaggacggc gacatcgtca
    26461 ccgtccccgt cgacgatcag ggcgccgcgc tcgatccgaa gacctccgct ctggacggcg
    26521 tcaagggcgc ctccgagccc tccatcaccg ccgtcggcga cgtgccggtc gtgctgaacc
    26581 cctcggacgg gaccgtcctg gccgccggcg tgcgcaccga gctcaagggt gcgaaggatg
    26641 ccgtgctgca gcagacatcc gccgccacgg atgccgtggc cgtcgcgacc tccgccggtc
    26701 tggtccgcgt tcccctgcac ggcggcgagg ccacgacggt gaaggcgggc ggcacgggca
    26761 cgcccgcggc cccggttttc ctcgagggct gcacctacgg cgcctgggga ggctcggccc
    26821 ggttcgtgcg cgactgcgcg ggggagcgcg acgacgtcac ggcgaagatt cccggtgcgg
    26881 agagcagcac ccgtctgatg ttccgcgtca atcgcgacgt gatcgtgctc aacgacgtga
    26941 tcggcggcga ggcctggctg gccgacgaga gcctgcagcg cgtcgacgac tggagcatcc
    27001 tgaccccgcc cgagggtgag accgaggaca ccgacgagtc cactcaggag accgtcgaga
    27061 cctcgctccc cgagcgcaag gaggagaaca cgcctccggt ggcggaggac gacaggctcg
    27121 gcgttcgtcc cggaggcacg acgctgctgc cggtgctcga caacgacaac gactccgacg
    27181 gcgacgtgct ggtcgcgact ctggccggcg cgcagccgtc ggtcggcgag gtgcagccga
    27241 tcctgaacgg tgcggcgctg cagatcatgg cacccgacga tgcgtcggga acagcggcgt
    27301 tcacctacca ggtcgatgac ggccgggcca acggcaccga caccgccacg gtcaccgcga
    27361 cggtgtacga ctgggacacc aacaccgcgc ccaagcagaa gcgcaagccc aagacctccg
    27421 tcgagtcggg cggaacgctg tcgtacaacg tgctgcagga ctggatcgac cccgagggcg
    27481 acgacctcta cctcaagagc gtcgtcgcgg ccgacggcga cgaggtcgag ttcacgaccg
    27541 acggccagat gacctaccgg gccgtcggca gcctgcaggg ccgcaaggag atcgaggtcg
    27601 ctgtcgcgga tgccctgggc gaggtcgcca ccggcacgat catcctcgac gtcaagcccg
    27661 ctgggacgac cgcgccggtc accaacgccg accacatcgt gacccgggtg ggcgagaccg
    27721 cgacggtggc gccgctggcc aacgactcca gctccggccg cgacgagctg cggctcaccc
    27781 gcgtcgaacc gctcgacggc gtgaccgtcc tgccggacta cccgaagaag cagttcgcct
    27841 tcaagtcgaa cgcgccgggc gtctactacg tgcagtacca ggcgtcggcg ggccaggcgg
    27901 atgccaaggg catcgtgcgc gtcgatgtgc tcgacaagca ggaatcggag ctccccccgg
    27961 tcgccgtgcg cgatgtcgcc ctgctgccga cgggcaagga ggcgctggtc ggcgtgctcg
    28021 cgaacgattc tgaccccgcg ggcggcgtgc tggtcgtgca atccgtctcc gtgcctccgc
    28081 acgcgggcgt cgcggtgtcg gtgctgaacc acgagacgct gcggatcacc gatcagggcg
    28141 ctctcgacga gccggtcaag atcacgtacc gcatctcgaa cggatcgaag tcggcggagg
    28201 gcgacgtgac ggtggtgccg atccctgctc cgtccaagat cacgccgccg gtcacgcacg
    28261 acgatgaggc cgtcgtgcgc gtcggcgacg tcgtgacgat ccccgtgctc gccaacgacg
    28321 tgcaccccaa cggcgacgtg atgcacgtcg caccggacct ggtcgagccg ctggtggacc
    28381 cgaaggacgg cgaggcgttc gtctcgcagg acaccgtgcg cttccgtgcc ggcaagcagc
    28441 ccggcacggt ctacctcacc tacgaggccg tcgactcgat ggggcagaag gcgggcggtc
    28501 acgtcaccgt gcaggtcctc cccgtcgacg gcgaggtcaa cgcaccgccg cgtccgcgcg
    28561 acctgaccgc gcgcgtcctg gccggtacca gcatccgcat cccggtcccg ctcgacggga
    28621 tcgacgtgga cggcgactcg gtcgaactgc tcggccagga catcgccccg accaaggggc
    28681 ggatcaccga gaccggcggc gacttcctgg agtacgaggc cttcaagggc tccacaggcg
    28741 tcgacaccat cgtctaccgg gtgcgcgatc gcttgggagg cgagggcacg gcgacgatcc
    28801 gggtcggcat cgcccccgca gaggcgacca accaggcgcc gttcgcggtg aaggacaccg
    28861 tggtggtgcg tcccggccgc tcggtggcgg tcccggtgct ggcgaacgac tccgatcccg
    28921 agggcgatga gatccgcctg gtcaaggacg gcatcgagac gccgaaggac tccggcatca
    28981 cggcgagggc gtccggcgac cgcgtcgtcg tcaaggtccc cgaccgcgag ctcgatgcat
    29041 cgctgcagta cacgatccgc gactccaagg gcgccgaggc ggacgctccg atcctcgtca
    29101 ccgtcgacaa ggacgtcccg ctcatgaccc cggtcgcacg cgacgaccgg ccgcgcgtcg
    29161 aggacctcga ggactcgctc tccgccgaca tcgacatcct cgccaacgac gaggacccgg
    29221 acggcacggt cgagggactg aaggtggccg tcgacggcgg tgcgaagctg gtcggcgacc
    29281 gcaagatccg cgtgagcgtg accgaccagc gtcagctcat ccgctacacc gtgaccgacc
    29341 aggacgagca gacagcttcg gcgttcatct tcgtccccgc gctcagcgag ctgcgtccgg
    29401 cgctgcgctc gaccaagccg gtcgaggtca agagcggcga gacgaaggtg ctcgagctcg
    29461 ccgactacgt caccgtcgcc ggcggcggga acgtgcgtct cacggaggcg tcgaaggtga
    29521 gcgccggtca cggcaacggg gacaccctga tccaggacga gaccacgctg gtctacacgt
    29581 ccgagaaggg ctacttcggc aaggactcgc tgagcttcga ggtcacggac ggcaagaccg
    29641 tcgaagaccc cgagggtcgc aagagcaccc tgtcgatccc gatcacggtg ctgccgcccg
    29701 acaaccagcc gccggtgttc gtgaacggcc agatcgacgt cgccccgggc gaggaggcga
    29761 cgtccatcga cctcgccgcg ctgaccaccg atccggacaa gggcgatgcg gggaagatcc
    29821 gctacagcat caccgatcgc cccggcgacg gcctgaacgc gcgcctggac gggagcaagc
    29881 tcctggtcga tgcgtcggcg accaccccca agggcacgac cgcgacggtc gggctgagca
    29941 tcacggacgg cgccacggag gctgtcgaag gcagcgtgac ggtcacggtg acggcatcca
    30001 ctcgcccgct cgccgtcgcc ggtcccgaca acgtcgacga ggcggaccag ggcaagacga
    30061 tcaccgtccc ggtgctgcag aacgacatca atcccttcga ggccgacggg aagccgctga
    30121 aggtcctctc cgcggatcgc gagagcggca acggcgacgc gagggtcgtg ggagaccgcg
    30181 tcgaggtcac cccggcatcg gacttcgtgg gcgtgatggt ggtgcgctac cgcatccagg
    30241 atgcgacgaa ggacgcagat cgtgaggtcg acggccgcat caccgtgacg gtgcagggtg
    30301 tgccggatgc tccgggtgtg ccgcgggtga cgacggtcga ggatcgcacg gtggtgctga
    30361 actggtcagc accgtcgaac aacggcgccg agatcgacca ctacacggtg acctctgcgg
    30421 gcgggcgtcc gtacaccaag acgtgcacgt cgacgacgtg caccctggac gggctgacga
    30481 acaacgtcga gtacaccttc cgggtgaccg cgcacaaccg cgtcggcgac ggccccgcgt
    30541 ctccggcatc cgagcccgca cgcccggatg tgcgtccgga gacgccgctg ccgccgaagc
    30601 tggtgttcgg tgacaagtct ctgcaggtgt ggtggactcc tccgcagtcg aacggttcgc
    30661 cggtggatgc gtacacgctg cagatctcgc cggcgccgtt gagcggttcc tcggagcgga
    30721 aggtcggcaa cgtcacgtct ctgacctggg acggtctgga gaacggcacg aactatcagg
    30781 tgcgggtcca ggcgcacaac caggctcccg acccctccga ctacagcgtc tggtccctgg
    30841 gcgagactcc ggcaggcgtc ccgggcacgg tcgcggctcc gacggtgagc agtgctccgt
    30901 cggtgggcag cgaggcgcag atgacggtgc gctgggccga tgccaacccg aacggtgacg
    30961 cggtcaagaa ctacgacctg ctgatctacc gcggcggttc gctgttccag accctggcgg
    31021 tgggcaccgc gacgtcgaag acggtctccg tcccgacgaa ctcctccgac tacacctacg
    31081 cggtgaaggc gacgaacaag gccgggacga gcgccgcgag cccgcagtcc actccgcagc
    31141 gcgcgttcgg atccccgggc gccccgacga acgtgaaggc gacgccgggg gatcagaaga
    31201 tcgccgtgac cttcaccaag cccgactaca acggggcgaa ggacggtgag gtccgctacc
    31261 agtactcgct ctcgggagct gcgtggacga actggaacgg cagcagcacg atcccggcga
    31321 acaacggaac cgactacacc gtcaagctgc gcgcgtactc ggtggtcggc ggtcagcagt
    31381 cccagccggg tgcggcgtcc gctgcatcca acactgtgcg tccctatggc actccgcatg
    31441 cgcccaccgg ttcggcgtcc cagtccggcg atcagcagat caagctcacc tggaacgccg
    31501 gcggctccga caacggctac cccgtcacca cgtacatcag tgtggacggc ggtggctggc
    31561 agaaggtggc caccaccggc agcaggatcg tcggcaacgg ctacaaccag acccacacga
    31621 tccgggtcaa ggccgtcgcc aaccccggtg gggagtcgct ctcgccgacg tacagcgcca
    31681 ccacgagtgc gccgccttcg cccaagatct gggtcacgaa gggcgactac gtgagcggct
    31741 gcgtgaacgg ctgctacaag ttcaaggtca actgggagaa cgccaacctc ggcagcgtca
    31801 ccgtcaagtg catgaaggac ggcggacagg tcggcaacta cgctcacccg gtgaccatga
    31861 acggcaccgg aagcagcccc tccctggact gctggcaggg cagcgacaac gtcaacgtgt
    31921 gggtcgacgt cgtcggatgg ggcgacagcg tcgacaccga gaaacggtac tgggccaaat
    31981 agccgggccc gacgcgacgc agtccgacga cacccgcggg ggagcgccca ggtgcacggg
    32041 tagtcctgcc catgccccag cagtggggcg gggccggtat gccggttaca atgacacggg
    32101 cctatcccgg ggggagacaa cggaatgaga ctgctgtcgt gggtgcgcgc gcgtccgaag
    32161 gcgctcgctg cgacggccgg cgtcgtcgtc ggcagcgtgg tgctcaccgg catggccctc
    32221 gcctatgacg gactccccac cgctaaggtc gacctcaacg acgcgggtgt ctggctgacc
    32281 aagtcctcgg cactgctcgt ggggcacttc aatcacgagt ccaccgtgct cgacggcggt
    32341 ctgcgcacga gcggcgagaa ctacgacatc ctgcagcaga gtcagaccgt gctcgtggtc
    32401 gaccacggca actccacgct gaccgcgatc gacaccgcca acgtctcgct caccgacagc
    32461 accacgatcc ccggcgacgc gaaagtcgcg ctcggcgatc acaccgcggc cgtgctggat
    32521 cgcaagtcgg gtgcgctgtg ggtgctcggc gctgccgacg tcgccggttt cgacgtgaag
    32581 gcgaccgatc cgatcgcgaa gctcggcaag gacgccgatg tcacggtcgg ccaggacggc
    32641 accgtgttcg ccgtgtcggc ggaggacggc gagatcatca cgatccctgt cgacgctcag
    32701 ggctcgccgc tggaacccaa gacctcctcc ctggacggcg tcaaggacgc gtccgagccc
    32761 acgatcacgg ccgtcggtga cgtgcccgtg gtgctcaacc cctccgacgg cgtcgtcgcg
    32821 gcgcccggcg gggtgcacgc gcggatcgac gacgcgaagg atgccgtgct gcagcaggca
    32881 tccgccgcga ccgatgccgt cgcagtcgcg acgccgacgg gactgatccg cgtgccgctg
    32941 aacggcggcg aggccagcac cgtcaaggcc gggggatcgg gaatccccgc ggcgccggtg
    33001 ttcctccagg gctgcaccta cggcgcctgg ggcggctcgg cgcggttcct ccgcgactgc
    33061 gggggcaagc aggacgatgt ctccgcgaag atccccggcg gtgagcgcag ctcgcgcctg
    33121 atgttccggg tgaaccgcga cgtgatcgtg ctcaacgacg tgatcggagg cgaggcctgg
    33181 ctggccgacg acagccttca gcgcgtcgac gactggagca tcctgacgcc tcccgagggc
    33241 gaggccgagg accaggagga caccacgcag gagacggtgg agacctcgct tccggagcgc
    33301 aagcccgaga acacgccccc ggtggcggag gacgacaagc tcggcgtgcg cccggggggc
    33361 accaccatgc tgccggtgct ggacaacgac aacgaccccg acggcgacgt gctggtcgcg
    33421 accgtcgccg gcgctcagcc gtccatcggc gaggtgcagc cgatcctgaa cggcggagcc
    33481 ctgcagatca tggcgccgaa ggatgccagc ggcacggcgt ccttcacgta ccaggtcgac
    33541 gacggccgtg agaacggcac cgacaccgcc acggtcaccg cgacggtgta cgactgggac
    33601 accaacagcg ctcccaaacc caagcgcaag accaagctgt cggtcgaggc cggcggcacg
    33661 ctgtcgtaca acgtgctccc cgactggatc gaccccgagg gcgacgacgt ctacctgcag
    33721 caggtcgtgg cggcacccgg cgatgaggtc gagttcacca ccgacggcca gatcacgtat
    33781 cgcgccgtgg gcagcctgca gggccgcaag gaggtctcga tcgtcgtggc ggatgccctc
    33841 ggcgagaccg cgaacggcac gatccagctc gatgtgaaac cggccggcgg cacggtgccg
    33901 gtcacgaacg ccgaccacgt cgtcacccgt gtcggggaga ccgcgacggt ctctcccacc
    33961 gcgaacgaca ccagcaccgc acgcgacgac ctgcgcctga cgcgcgtcga gccgctcagc
    34021 ggcgtgacga tcgtgccgga ctacccgaag aagcagttca cgttcgagtc gaacgcgccg
    34081 ggcgtctatt acgtgcagta ccaggtgtcg gcgggtcagg cggatgccaa gggcatcgtg
    34141 cgcatcgatg tgctcgacaa gcaggagtcc gagctgccgc ccgtcgcggt gcgcgacgtg
    34201 gccctgctcc ccaagggcag cgaggcgctg gtcggcgtgc tcgcgaacga ctccgaccct
    34261 gccggcggcg tgctggtcgt gcagtcggtc acggtcccgc cgcacagcgg catcgacgtc
    34321 tcggtgctga accacgagac gctccgcatc ggcgatcagg gcgctctgga ccagcccgtg
    34381 aagatcacct acacgatctc gaacggctcc aagtcggcgc agggcgacgt cacggtcatc
    34441 ccgatcccgg cgccctccaa gatcgcggct ccggtcacgc acgacgacga agcggtcgtg
    34501 cgcgtcggcg acgtcgtgac gatccccgtg ctcgagaacg acgtgcatcc gaacggcgag
    34561 gtcatgcatg tcgcaccgaa gctgatcgag ccgctggtcg ccccgaagga cggcgaggcg
    34621 ttcgtctcgc aggacaccgt gcgcttccgc gccgcgaacc agcccggcac cgtctacctc
    34681 acctacgagg ccgtcgacgc gatggggcag aaggccggcg gtcacatcac gatccaggtg
    34741 ctggcgaagg atgccgacgt caacgcgcct ccgcacccgc atgacctcac ggcgcgggca
    34801 ctggcgggca ccagcatccg catcccgatc ccgctcgacg ggatcgacac cgacggcgac
    34861 tcggtcgagc tgctcggtca ggacgtcgcg ccgatcaagg gccgcatcac cgagaccggt
    34921 ggcaacttcc tgcagtacga cgcgttccag ggctccaccg gcgtcgacac cgtcgtgtac
    34981 cgggtgcgcg atcgcctcgg cgccgagggc acggccacga tccgcatcgg catcgcacct
    35041 cccgaggcga ccaaccaggc cccgtacgcc gtgaaggatg ccgtggtcgt gcgtcccggc
    35101 cgctcggtgg cggtcccggt gctcgcgaac gactccgatc ccgaaggcga tgagatccgc
    35161 ctggtgaagg acggcatcga agtccccgag gactccggca tgaccgcgaa ggcatccggc
    35221 gaccgcgtgg tcgtgaaggt gccggacaag gagatggagg cgtcgctgga atacacgatc
    35281 cgcgacgcga agggcgccga ggcgaccgcc cccatcctcg tgacggtgga caaggatgtt
    35341 ccgctgatcc cgcccatcgc ccgtgacgac cggcttcggg tcgaggatct gaaggactct
    35401 ctcaccgccg acatcgacat cctcgccaac gacgaggacc ccgacggcac ggtcgacggg
    35461 ctcaaggtca cggtcgacgc cggcgccaag ctcgtcgacg cccgcaaggc ccgcgtcacg
    35521 gtgacagacc agcgtcagct gatccggtac acgatcaccg accaggacga gcaggaagcg
    35581 tccgcgttca tcttcgtgcc ctcgctcgac gagctcgggc cggcgctgcg ctcgaccaag
    35641 ccggtcgagg tcaagagcgg cgagacgaag cagctcaagc tctccgacta tgtcgcggtc
    35701 gcgggtggcg gcagcgtgcg cctgaccgag gcatccaagg tcagtgccgc ccatgccaac
    35761 ggcgacaacc tgatcaagga cgagaccacg ctcgtgtaca cgtcgaagaa gggcttcttc
    35821 ggcgacgacg cgctgaactt cgaggtcacc gacggcaaga cgctggagga cccgaagggc
    35881 cgccgcagca cgctgtcgat cccgatcacc gtgctgcccc cggagaacca gccgcccgtg
    35941 ttcgtcaacg gtcaggtcga cgtcgcgccg ggggagaagg cgacgtcgct ggatctcgcc
    36001 gcgctcacca ccgacccgga caaggcggat gccgggaaga tccagtaccg gatctcgaag
    36061 cagcccggcg acggcatcaa cgcgcggctg gacggcaccc ggctcctcgt ggatgcctcc
    36121 tcgagcaccc ccaagggcac gaacgcgatg gtgtccctga gcatcaccga cggcacgacc
    36181 gaacccgtcg agggcaacgt cgccgtgaac gtcacggcat ccacgcgcag cctcgccgtc
    36241 gcgagccccg acaccatcga cgaggcggat cagggcaaga cctacaagat cccggtcctg
    36301 accaacgaca tcaacccgtt cgccgatgag ggcacgcccc tgaagctgct ctcggccgag
    36361 cgcgagagcg gcgacggcac cgcgcgcgtg gtcggcgacc aggtggagat cacgcccggt
    36421 gcgaagttcg tgggcgtcct ggtggtgcgc taccgcatcc aggatgcgac gaaggacccc
    36481 gaccgtgagg tcgacggccg catcaccgtg acggtgcagg gcgtccccga cgcacccggt
    36541 gtgccgcggg tgacgaccgt cgaggaccgc accgtggtgc tgaactggtc cgcgccgtcg
    36601 aacaacggcg cggagatcga tcactacacc gtcacatccg tcggcggccg gccgtacacg
    36661 aagacctgcc agaccaccac gtgcacgctg gacgggctcg tcaacaacgt cgagtacacg
    36721 ttcaaggtca cggcgcacaa ccgtgtcggc gacgggccgg cctccccgct gtcggagccg
    36781 gcgcggcccg atgtgcggcc cgagacccct cagccgccga agctggcatt cggcgacaag
    36841 tctctgcagg tgtcgtggac tcctccgcag tcgaacggtt ctccggtcga ctcctacacg
    36901 ctgcagatct cgcccgcccc gctcagcggt tcttcggagc gcaaggtcgg caatgtcacc
    36961 tcgctgacct gggacggtct ggagaacgga acgaactacc aggtgcgggt gcaggcgcac
    37021 aaccaggctc ccgacccctc cagttacagc atctggtcgc tcggagagac gcccgccgga
    37081 aagcccggca cggtcgccgc gccgaccgtg aacagcgcgc cgtccgtcgg caccgaagcc
    37141 cagatgaccg tccggtggac ggcggcggac ccgaacggcg acgcgatcaa gaactacgat
    37201 ctgctcgtgt accgcggcgg tgcgctctac cagaccctct ctgccgggac cgcgacgtcg
    37261 aagacggtga ccgtcccgac gaactcctcc gactacacct acgcggtgaa ggcgacgaac
    37321 aaggccggca cgaacgatgc cggtccgcag tccgcaccgc agcgcgcgtt cggcaagccg
    37381 ggggcgccgt cgaatgtcgt gatcagggaa ggcgatcgtc aggtcgccat cgcctcgtac
    37441 acgaagcccg actacaacgg cgccaacgag agcgagatcc gatatcagta ctcgctcagc
    37501 ggcgggacct ggacgaactg ggacggcagc agtgtgatcg ccgccgacaa cggcaacggc
    37561 tataccgtgc ggctgcgcgc gtactccgtg gtcggcggac agcagtcgca ggccggggac
    37621 ccgtccgccc cgtcgaacaa cgtgatcccc tatggtgccc ccttcgctcc gaccggatcg
    37681 gcatcgaagt cgggtgacaa ggccatcctg ctgacctgga acgccaacgg ctcgtacaac
    37741 ggccgaccca tcacgacgta catcaacgtc gacggcaatg gatgggagca ggtggcgaag
    37801 agcggcagcc gcacggtcgg caacggctac gaccagacgc acaagatccg ggtcaaggca
    37861 gtcgcgtcag agggtggcga ggcactgtcg ccggtgtacg acgcgaccac gagcccacag
    37921 ccgatcaagg cgagcctcgg acccggcgcc gcggtaagcg gatgctccgc cgtgggcggc
    37981 ggcagctgcc acaagttcag gatcacgacg accagcgcct tcaaggccgg aacgtacacg
    38041 gtgaaatgct tcgccggcgg ttcggagttc ggaggctatg ccctgaagat cccttccgga
    38101 agctgggtgg acgtcgactg ctacaacgga ttctcccgcg acaagatggc caagatcgtc
    38161 ggcggcgatc cggccagtat cagcgccacc ttcggctagc cgaccaccag attcagactt
    38221 cccaccactc gagaagaaga gacaccgaca tgacaatgac ccccgaacag gccgcctggt
    38281 tccagagcac cttcacccgc ctggtggaga acatcgacaa ggcgctgcag ggcaagcccg
    38341 aggtcgtgag cctggtgctc tccgccatgc tcgcagaggg ccacgtgctg ctcgaggacg
    38401 cgcccggcac cggcaagacc agcctcgcca aggcgctggc cgccaccgtg cagggcacca
    38461 actcgcgcat ccagttcacc cccgaccttc tgccgtccga cgtgacgggc gtgacgatct
    38521 acgaccagca gaaccacaag ttcgagttcc acaagggacc gatcttcgcg tcgatcgtgc
    38581 tcgcggatga gatcaaccgc gcctcgccga agacccagtc ggcactgctg gaggtcatgg
    38641 aggagtcgcg cgtcaccgtc gacggcgtca ctcacgaggc cggccgtccg ttcctggtga
    38701 tcgcgaccca gaaccccgtc gagcaggccg gaacctacaa gctgcccgag gcgcagctgg
    38761 accgcttcct catcaagacc tcgatcggct accccagcct ggcggtcacc gagagcatcc
    38821 tcgccggtgc ggcgcagcgc aacccctccg cgggactcgc cgcggtgatc accaccagtg
    38881 ccgtcgccga catggcggac ctggcggcta ccgtgcacgt cgagcccgcc gtgctgcggt
    38941 acgccgccga gctcgccgag gcgacgcgcg gcgacagcgc catcaagctc ggcgtctcgg
    39001 tgcgcggtgc gatcgccatg atccgcatcg ccaaggtgtg ggccgccgcg cacggccgcc
    39061 actacgtgct gccggacgac atcaaggctc tcgcgcgtcc cgtgtggcag caccgtctca
    39121 tgctcgacgc ggaggcggag ttcgccggca ccaccgccga caccgtcatc gcacgggtcc
    39181 tcgacaccgt cgcggcaccc caggcgcgga cgaacgcctg atggcggcgg atgcgatcg
//
LOCUS       DATMSX010000556         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_83954,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000556 DATMSX010000000
VERSION     DATMSX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83954"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VN041_17630"
     CDS             <1..320
                     /locus_tag="VN041_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30904.1"
                     /translation="IGQRDSAAVEEMLAEDFVGPDGLDRKGARRLAVASFMRYSDLSV
                     TLGPLDLQTSAGHATVNFSAVLTGGSGQVLPDAARMYQVSTGWREEDGEWRMTSAKWT
                     PGL"
     gene            complement(451..3201)
                     /locus_tag="VN041_17635"
     CDS             complement(451..3201)
                     /locus_tag="VN041_17635"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWU30905.1"
                     /translation="MSTVNSFGAKSTLTVGSTDYEIFRIDTVAGHEKLPFSLKVLLEN
                     QLRTEDGKNVTKAQIEALGSWDATAEPNTEIQFTPARVLMQDFTGVPCIVDLATMREA
                     VTALGGDPSRINPLSPAEMVIDHSVIADLFGAENALQRNVEIEYERNGERYQFLRWGQ
                     TAFDDFKVVPPGTGIVHQVNIEHLAKVVYDRTVDGVLRAYPDTLVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPAGVTATDVVLTITDMLRQHGV
                     VGKFVEFYGAGVASVPLANRATIGNMSPEFGSTAAIFPVDDVTLDYLRLTGRSDEAIA
                     LVESYAKIQGLWHDAAKEPVYSEYMELDLGTVVPSIAGPKRPQDRILLSEAKTQFEKD
                     ILNYATPSTSDELADLESKHSFPASDPGQVPGEEEVHTRPVHISSGAPAASSKPVPVT
                     TSAGDQYILDNGAVTLAAITSCTNTSNPSVMVAAGLVARKALKKGLKQKPWVKTTLAP
                     GSKVVTDYYEKSGLNEDLEGLGFYTVGYGCTVCIGNSGPLIDEVSAAVNEHDLAVTAV
                     LSGNRNFEGRISPDVKMNYLASPPLVIAYALAGSMHFDFENDALGQDTEGNDVFLRDI
                     WPTSEEVQEIIDSSISREQFIKQYATVFDGDERWTGLPTPTGPIFEWDEDSTYVRKAP
                     YFDGMTMELTPVTDITGARVMAALGDSVTTDHISPAGAIKPGTPAAQYLQEHGVERKD
                     FNSFGSRRGNHEVMIRGTFANIRLKNTLVKAVNDGQQIEGGYTRDFTQPGGPQAFIYD
                     ACMNYQEQGVPLVVFGGKEYGSGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMG
                     VLPLQFKDGENAQSHGLDGSEAIDVIGLDDGKAKTATVVAKKADGSETKFEVKVLLLT
                     PK"
     gene            complement(3376..>4000)
                     /locus_tag="VN041_17640"
     CDS             complement(3376..>4000)
                     /locus_tag="VN041_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HWU30906.1"
                     /translation="GLGADSSESTQKVVWTAMGGWRGILESVLPSLAFVVLFTVRPEP
                     LILSLGVSVGLAAVFTFIRLLQKSPPSAAIGGLVAAGAAAVLALWTGRGADNFVPGLI
                     TNAVYGTAMIVAALVGWSLIGLAVGFLMGEGTAWRQDRRKRRAFFWLGMAWGVLFFAR
                     LGVQLPLYLTDQVTLLGTLKLIMGLPLFAPLVAVTWLVVRALYRRGE"
BASE COUNT          630 a         1272 c         1410 g          688 t
ORIGIN      
        1 cgatcggtca gcgcgattcg gcggcggtgg aagagatgct ggccgaggat ttcgtcggtc
       61 ccgacgggct cgaccggaag ggcgcgcgcc ggctcgcggt ggcgagcttc atgcgctata
      121 gcgatttgag cgtgaccttg gggccgctgg atctgcagac gtcggccggg catgcgacgg
      181 tgaacttcag cgcggttttg accggtgggt cggggcaggt gttgcccgac gcggcgcgga
      241 tgtaccaagt cagtactggc tggcgcgaag aggacgggga atggcggatg acgtcggcga
      301 agtggacgcc ggggttgtag caggggcgat gacgtgtcct tctcccgctt gcgggagagg
      361 ggcttcaaag cccattgctc aggcggcttt cttcgccgca agttgccgca acacgtaatg
      421 caagatgccg ccatgccgga agtactccac ttacttcggc gtcaacagca acaccttcac
      481 ctcgaacttc gtctcgctgc cgtcggcctt cttggccacc accgtcgcgg tcttggcctt
      541 gccgtcgtcc agcccgatca cgtcgatcgc ctccgagccg tccagcccgt ggctctgcgc
      601 gttctcgccg tccttgaact gcaacggcag cacgcccatg ccgaccaggt tggagcggtg
      661 gatgcgctcg aagctctcgg cgatcaccgc cttcacgccc agcaggttgg tgcccttggc
      721 cgcccagtcg cgcgaggatc cggagccgta ctccttgccg ccgaagacca ccagcggcac
      781 gccctgctcc tggtagttca tgcaggcgtc gtagatgaac gcctgcggac caccgggctg
      841 ggtgaagtcg cgggtgtacc cgccctcgat ctgctgaccg tcgttgacgg ccttgaccag
      901 cgtgttcttc aggcggatgt tcgcgaaggt gccgcggatc atcacctcgt ggttgcctcg
      961 acgcgatccg aaggagttga agtccttgcg ctccacaccg tgctcctgca ggtactgcgc
     1021 agcaggggtg cccggcttga tggcgccagc cggcgagatg tggtcagtgg tgaccgagtc
     1081 gcccagggcc gccatcacgc gggcgccggt gatgtcggtg accggcgtga gctccatggt
     1141 catgccgtcg aagtacggcg ccttgcgcac gtaggtcgag tcctcgtccc actcgaagat
     1201 cggaccggtc ggcgtgggca ggcccgtcca gcgctcgtcg ccgtcgaaca cggtggcgta
     1261 ctgcttgatg aactgctcac gcgagatcga tgagtcgatg atctcctgca cctcttcgga
     1321 ggtgggccag atgtcccgca ggaacacgtc gttgccctcg gtgtcctgac ccagcgcgtc
     1381 gttctcgaag tcgaagtgca tcgagccggc gagggcgtag gcgatgacca gcggcggcga
     1441 ggccaggtag ttcatcttca cgtcggggct gatgcggccc tcgaagttgc ggttgccgga
     1501 gagcacggcg gtgaccgcga ggtcgtgctc gttgacggcg gccgagacct catcgatcag
     1561 cgggcccgag ttgccgatgc agaccgtgca gccgtagccg accgtgtaga agcccaggcc
     1621 ctcgaggtcc tcgttcaggc ccgacttctc gtagtagtcg gtgacgacct tggagccggg
     1681 agccagcgtg gtcttgaccc acggcttctg cttcaggccc ttcttcagtg ccttgcgagc
     1741 gaccaggccg gccgcgacca tgacggaggg gttcgaggtg ttggtgcacg aggtgatcgc
     1801 cgcgagcgtc acggcgccgt tgtccaggat gtactggtcg ccggcggacg tggtcacggg
     1861 aaccggcttg gacgacgcgg cgggcgcgcc gctgctgatg tgcaccggac gcgtgtgcac
     1921 ctcctcctca ccggggacct gaccggggtc ggatgccggg aaggagtgct tcgactcgag
     1981 gtcggccagc tcgtccgagg tggacggggt ggcgtagttg aggatgtcct tctcgaactg
     2041 cgtcttcgcc tcggagagca ggatgcggtc ctgcgggcgc ttcgggccgg cgatcgaggg
     2101 gaccacggtg ccgaggtcga gctccatgta ctcgctgtac accggctcct tggcggcgtc
     2161 gtgccagagg ccctggatct tggcgtacga ctcgaccagc gcgatcgcct cgtcgctgcg
     2221 gccggtgagg cgcaggtagt cgagggtgac gtcgtccaca gggaagatcg cggcggtgga
     2281 gccgaactcc ggcgacatgt ttccgatggt ggcgcggttg gcgagcggca cggatgccac
     2341 accggcgccg tagaactcga cgaacttgcc gaccacgccg tgctggcgca gcatgtcggt
     2401 gatcgtcagc acgacatcgg tcgcggtgac gccggcgggg atctcgccgg tgagcttgaa
     2461 gccgacgacg cgcgggatga gcatggagac cggctggccc agcatggccg cctcggcctc
     2521 gatgccgccg acgccccagc ccagcacgcc caggccgttg accatggtgg tgtgcgagtc
     2581 ggtgccgacg agcgtgtcgg ggtaggcgcg cagcacgccg tcgacggtgc ggtcgtagac
     2641 gaccttggcg aggtgctcga tgttcacctg gtgcacgatg ccggtcccgg gcgggacgac
     2701 cttgaagtcg tcgaaggcgg tctggcccca gcgcaggaac tggtagcgct caccgttgcg
     2761 ctcgtactcg atctcgacgt tgcgctgcag cgcgttctcc gcgccgaaga ggtcggcgat
     2821 cacggagtgg tcgatgacca tctcggcggg cgagagcggg ttgatgcggc tcgggtcgcc
     2881 gccgagggcg gtgacggcct cgcgcatggt ggcgaggtcg acgatgcagg gcacgccggt
     2941 gaagtcctgc atgaggacgc gggcgggcgt gaactggatc tcggtgttgg gctcggccgt
     3001 ggcatcccac gaacccagcg cctcgatctg cgccttcgtg acgttcttgc cgtcctcggt
     3061 gcgcagctgg ttctccagaa gcaccttcag gctgaagggg agcttctcgt gaccggcgac
     3121 cgtgtcgatg cggaagatct cgtagtcggt gctgccgacc gtcagggtgc tcttggcacc
     3181 gaagctgttc accgtggaca cggatccgtc tccttctgtt cggatgggag cgacaggcga
     3241 ctccatcttg ctcgcctccg gatgccgcgg ctagcaaggt tcgccttacc agtgtgctcc
     3301 gcggaatcgc tcggcaaaag cccgagattt atcttgatgt cgagataaat ctaccagagg
     3361 tgaacggcgc ctgcgtcact cgccgcgccg atacagcgcc cggacgacga gccaggtgac
     3421 ggcgacgagc ggcgcgaaca gcggcagccc catgatgagc ttcagcgttc ccagaagggt
     3481 cacctgatcg gtgagataga gcggcagctg cacgcccagg cgcgcgaaga agagcacgcc
     3541 ccatgccatg ccgagccaga agaacgcccg ccgcttgcgg cggtcctgac gccacgccgt
     3601 gccctctccc atcaggaagc cgacggccag cccgatcagc gaccagccca ccagcgcggc
     3661 cacgatcatg gcagtgccgt ataccgcgtt cgtgatcagc ccgggcacga agttgtccgc
     3721 tccgcgtccg gtccacagcg ccagcaccgc cgcggcgccg gcggcgacga gaccgccgat
     3781 cgcggcggac ggcggcgact tctgcagaag acggatgaac gtgaacactg cggccaggcc
     3841 gaccgacacg ccgagcgaga ggatcagcgg ctcggggcgc acggtgaaga ggacgacgaa
     3901 ggcgaggctc ggcaggaccg actccaggat gccgcgccag ccgcccatcg cggtccagac
     3961 gaccttctgc gtgctctcgc tgctgtccgc gccgagtccg
//
LOCUS       DATMSX010000557         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_103365,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000557 DATMSX010000000
VERSION     DATMSX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103365"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1372)
                     /locus_tag="VN041_17645"
     CDS             complement(161..1372)
                     /locus_tag="VN041_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWU30907.1"
                     /translation="MSWLIGSLADAWAEIRVHKLRVLLSLIGIAVSVAALTAVVAVSE
                     LAMQSQTEEMDRYGGREATIAVTAMGENGPVDAAAFDERFTQVSERYGFSDVARIAEG
                     LMLPVQAPDGLHDTSARLLDPAYSRIHREKLLTGREFRADDAEALAPPVIVSEALWKV
                     LGSVPMAQHPTLTLAGPAGGTYQIVGVRPRQGTWDEEKRVDVLFDSYASRVDRLPEDA
                     QVRYDIWVGRKDVAAVGPALAMDLRAGLPEGQQVIVNRTDWGANPGYLEGQRMVELLI
                     GGVAALILGLGALSLINVQLVAMRQRVREIGVRRAFGATAGRIFTTVLLESLVATTVA
                     GVIGIAIVVTALRMPMLTEWLFPALVDVPPFPMRAAITGLVAAVIVGAVAGFVPALVA
                     LRVKVIDAIRF"
     gene            complement(1369..2181)
                     /locus_tag="VN041_17650"
     CDS             complement(1369..2181)
                     /locus_tag="VN041_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30908.1"
                     /translation="MTLVALQDVTKSVLLPDDSTLEILRGVTLEVGAGDHVSIVGRSG
                     SGKSTLLNILGMLDTPTTGTVSFAGREVRRMRQGRLDRLRGDHVGFVFQQFNLLPGRT
                     ALDNVMMPLGHAKGRLFWQRRRIAADMLERVGLGHRMEQIADRLSGGEQQRVAIARAL
                     VRRPVLILADEPTGALDIDTGASVMALLDEVATETDAALVTITHDLHVAARARRHYRL
                     DGGVLATADLRRAFEASTLQDDAPAGLALAASVPAGDVPADAVAADAAERAS"
     gene            complement(2178..3107)
                     /locus_tag="VN041_17655"
     CDS             complement(2178..3107)
                     /locus_tag="VN041_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWU30909.1"
                     /translation="MVVWRRVILPLVFVLVLGAAAAALVKLAFFPDVSQAAPADPSAG
                     VADPITAVERGSVVSALKLDATIARDEPYAVRSETDGTVTAVHVADGAAVAKGQLLVT
                     IKQVDPAKTVELRAPEAGDISELALVKGQTTSVGGEVLKLTPARHHVLATVKPAELYR
                     LVNAPTEATVSINGGPAPFTCTGVGVQIAEDGTASIRCAVPGDQKVFAGLPASVELAL
                     GKVDDALVIPVTAVKGGAGSGTVWVDAGDGAKPEERKVTLGVNDGEKVEVTDGLAEGD
                     SIRQFVPGFAAPSEEECYDDGQGGQYCESGTSW"
     gene            complement(3256..3480)
                     /locus_tag="VN041_17660"
     CDS             complement(3256..3480)
                     /locus_tag="VN041_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HWU30910.1"
                     /translation="MDDSLRTDERLCSKVACAREAVATLTFDYGDQMAALGPLGPGGD
                     PHAHDLCALHAERLSVPAGWLVVRHEAMRG"
BASE COUNT          521 a         1348 c         1258 g          501 t
ORIGIN      
        1 gacggaggag ctgctgcgcg gggtgaccgt gctgcgccgc cagcgcgagc agcgcgcgct
       61 ggtcatcgcc gacgagcgcg ccgagcggct caccgggcgg cgcgtacggg tctgacccac
      121 agcatccgct ctgcgtccga gctgggcgac cggagacgcc tcagaaccgg atcgcgtcga
      181 tcaccttcac ccgcagcgcg accagcgccg ggacgaaccc ggcgacggct ccgacgatca
      241 cggcggccac gagcccggtg atcgccgcgc gcatggggaa cggcggcacg tccacgagcg
      301 ccgggaacag ccactcggtc agcatcggca tccgcagcgc cgtgacgacg atcgcgatcc
      361 cgatcacccc ggcgacggtc gtggcgacca ggctctccag cagcaccgtg gtgaagatcc
      421 gccccgcagt ggctccgaac gcccgccgca cgccgatctc gcgcacccgc tgccgcatcg
      481 cgaccagctg cacgttgatg aggctcagcg caccgagacc gaggatcagg gccgcgacgc
      541 cgccgatcag gagctcgacc atgcgctgac cctccaggta gcccgggttc gcaccccagt
      601 ccgtgcgatt gacgatcacc tgctgcccct cagggaggcc ggcgcgcagg tccatcgcga
      661 gggcggggcc gacggcggcg acgtccttcc tgccgaccca gatgtcgtag cgcacctgcg
      721 cgtcctcggg cagcctgtcc acgcgcgaag cgtaggagtc gaagagcacg tcgacgcgct
      781 tctcctcgtc ccacgtcccc tgccggggtc gcactccgac gatctggtac gtgccgccgg
      841 cgggaccggc cagggtgagc gtcggatgct gcgccatcgg cacgctgccg agcaccttcc
      901 agagcgcttc cgacacgatc acgggcggag cgagcgcctc ggcgtcgtcg gcgcggaact
      961 cgcggcccgt gagcagcttc tcgcggtgga tcctggagta cgcgggatcc agcagccgcg
     1021 ccgaggtgtc gtgcagaccg tccggtgcct ggaccgggag catcagcccc tcggcgatgc
     1081 gggccacatc actgaacccg tagcgctcgg acacctgggt gaagcgctcg tcgaaggcgg
     1141 cggcgtcgac cgggccgttc tcccccatcg cggtgacggc gatggtggcc tcgcggccgc
     1201 cgtagcggtc catctcctcg gtctgcgact gcatggcgag ctccgacacc gccacgaccg
     1261 cggtcagcgc cgcgaccgac acggcgatgc cgatcaggct gagcaggacg cgcagcttgt
     1321 gcacgcggat ctccgcccag gcatcggcga gcgatccgat cagccagctc atgacgcccg
     1381 ctccgcagca tccgccgcga cggcgtccgc aggtacgtca ccggccggca cgctcgcggc
     1441 gagagcgaga cccgccggcg catcgtcctg cagagtggac gcctcgaagg cgcgacgcag
     1501 atcggcggtg gcgagcaccc cgccgtcgag ccggtagtgc cgccgagcgc gggctgccac
     1561 gtgcagatcg tgcgtgatcg tgaccagtgc ggcgtccgtc tcggtggcga cctcatccag
     1621 cagcgccatc acgctggcac cggtgtcgat gtccagggcg cccgtgggct cgtcggcgag
     1681 gatcagcacc ggcctgcgca ccagcgccct ggcgatggcg acgcgctgct gctcgccgcc
     1741 ggagagccgg tcggcgatct gctccatgcg gtgcccgaga ccgacgcgct ccagcatgtc
     1801 ggcggcgata cggcgccgct gccagaacag ccggcccttc gcgtgcccca gcggcatcat
     1861 cacgttgtcc agcgcggtgc ggccgggcag caggttgaac tgctggaaca cgaacccgac
     1921 gtggtctccg cgcagccggt ccaggcgccc ctgcctcatg cgccgcacct cgcggccggc
     1981 gaaggacacc gtgccggttg tgggcgtgtc cagcatgccg aggatgttca gcagcgtcga
     2041 cttcccggat ccggagcgcc cgacgatgga gacatggtcg ccggcgccga cctcgagcgt
     2101 gactccgcgc aggatctcca gcgtgctgtc gtccggcagg agcacgctct tggtgacgtc
     2161 ctgcagcgcg acgagcgtca ccagctcgtc cccgactcgc agtactggcc gccctgaccg
     2221 tcgtcgtagc actcctcctc ggagggcgcg gcgaagccgg ggacgaactg gcggatgctg
     2281 tcgccttcgg cgagcccgtc cgtgacctcc accttctcgc cgtcgttcac gccgagcgtg
     2341 accttgcgct cctcgggctt ggccccgtcg ccggcatcca cccagacggt cccgctgccc
     2401 gcaccgccct tgacggcggt gacggggatc acgagcgcgt cgtcgacctt gccgagcgcg
     2461 agttccacgg acgcggggag cccggcgaac accttctggt cgcccggcac cgcgcagcgg
     2521 atgctcgccg tgccgtcctc ggcgatctgc acgcccactc cggtgcaggt gaacggcgcc
     2581 ggaccgccgt tgatgctgac ggtcgcctcg gtgggcgcgt tgacgagccg gtagagttcg
     2641 gccggcttga ccgtggccag cacgtggtgg cgcgcagggg tcagcttcag gacctctccc
     2701 ccgacgctgg tcgtctgccc cttcacgagg gccagctcgg agatgtcccc tgcctccggg
     2761 gcgcgaagct cgaccgtctt ggcaggatcc acctgcttga tcgtcacgag cagctgcccc
     2821 ttagcgacgg ccgccccgtc tgcgacgtgc accgccgtga ccgtgccgtc ggtctcgctg
     2881 cggactgcgt agggctcgtc gcgcgcgatg gtggcatcca gcttcagcgc gctgaccacc
     2941 gacccgcgct ccacggccgt gatcggatcg gcgacgccgg cggacggatc cgcgggagcg
     3001 gcctgcgaga catcggggaa gaacgcgagc ttgacgagcg ccgcggctgc tgcgcccaga
     3061 accaggacga acacgagcgg gaggatcaca cggcgccaga cgaccacggt tgcctttcct
     3121 gggcagccgc cctggcgccc ccttggcatc aggctagggg aaggctcgcc ggaaacggat
     3181 cagggttcac ccccatctgt cgtcgcgtcg cccctgagtg cgatcgacgc gcgccgccgg
     3241 cctcggcggg atgcgtcagc cgcgcatcgc ctcatgccgc acgacgagcc agccggcggg
     3301 aaccgagagc cgctccgcgt gcagtgcgca caggtcatgc gcgtgcgggt ctccgcccgg
     3361 accgagcggg ccgagtgccg ccatctggtc gccgtagtcg aaggtgagcg tggcgaccgc
     3421 ctcgcgcgcg caggccacct tggagcagag ccgttcgtcg gtgcggagtg agtcgtccat
     3481 ctccctcagc gtaggaccgc ggatgccgga tgcgcgggtg ccgcgcggca tccgcaccga
     3541 gccgccggga cttcgaagac gacctagact ttcagacatg ccccgccgtc gttcctcccg
     3601 cgctcccggc cgccgcggcg cccgccac
//
LOCUS       DATMSX010000558         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_46956,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000558 DATMSX010000000
VERSION     DATMSX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46956"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="ribB"
                     /locus_tag="VN041_17665"
     CDS             complement(<1..1005)
                     /gene="ribB"
                     /locus_tag="VN041_17665"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWU30911.1"
                     /translation="MSLSTIDAALDALRAGRPVIVADDENRENEGDIIISAELASPEV
                     MAFMVRWTSGLICAPMPADLADSLNLPPMVESNEDARSTAYTVSVDAAEGVTTGISAH
                     DRAHTLNVLADPSSTATSIIRPGHILPLRAVDGGVRERGGHTEAAVDLMKLAGLQPVG
                     AIAEVVAEDGSMMRLPGLKDLGARDGVPVITIEQLIAHLNEIDPQDGPVVSSRAGKRR
                     VSLRADATVPTDHGTFRFLAYKDRVTGTDHIAVVSGELGETALVRVHSECLTGEAFGS
                     LKCECGPQLDAALDAIEQEGGVVIYMRGHEGRGIGLINKLRAYSLQEEGLDTVDANLA
                     L"
     gene            complement(1002..1631)
                     /locus_tag="VN041_17670"
     CDS             complement(1002..1631)
                     /locus_tag="VN041_17670"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWU30912.1"
                     /translation="MFTGIIEEIGEIAGIAPSGDGWRLTVRAPKAASDAVHGESIAVS
                     GVCLTVVGSTADTFDADVMKQTLDVSALGALNVGSRVNIEKAMPVGARLGGHIVQGHV
                     DGTGAVLEVRPGDQWRVLRISLPADLAPLVVDKGSISVAGTSLTVSAVSDVDEPSAWF
                     EVSLIPETLEATILGALAPGDVVNLETDILARHVERLLAFRTLPEGGSR"
     gene            complement(1631..>1850)
                     /locus_tag="VN041_17675"
     CDS             complement(1631..>1850)
                     /locus_tag="VN041_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU30913.1"
                     /translation="GPTLASAFIAAGLADTVLAYLAPVLLGGGRLALTDIGVDTIADA
                     RRLTVDEWLPLGDDLLAVARPSVLKERS"
BASE COUNT          276 a          619 c          668 g          287 t
ORIGIN      
        1 cagtgcgaga ttcgcgtcga cggtgtcgag tccctcttcc tgaaggctgt aggcgcgcag
       61 cttgttgatc aggccgatcc cgcgaccctc gtggccgcgc atgtagatga cgaccccgcc
      121 ctcctgctcg atcgcgtcga gtgcggcgtc gagctgcggc ccgcactcgc acttgagcga
      181 tccgaacgcc tcacccgtga ggcactccga gtgcacgcgc accagggcgg tctctccgag
      241 ttcgccggag acgacggcga tgtgatcggt gccggtcacc cggtccttgt acgcgaggaa
      301 gcggaacgtg ccgtgatcgg tcggcacggt ggcgtctgca cgaagactca cacgccgctt
      361 gccggcacgg gaggacacga ccgggccgtc ctgcggatcg atctcgttca agtgcgcgat
      421 gagctgctcg atcgtgatga caggcacacc gtcgcgcgcg ccgaggtcct tcagcccggg
      481 caggcgcatc atgctgccgt cctctgcgac cacctctgcg atcgcgccga cgggctgcag
      541 cccggccagc ttcatcagat cgacagccgc ttcggtgtgt ccgccgcgct cgcgcacgcc
      601 gccgtcgacg gcgcgcagcg gcaggatgtg ccccgggcgg atgatgctcg tcgcggtgga
      661 ggacgggtcg gcgagcacgt tcagggtgtg cgcgcggtcg tgcgcgctga tgcccgtggt
      721 cacgccctcg gcagcatcca cgctgaccgt gtaggcggtt gagcgggcat cctcgttgct
      781 ctccaccatg ggcggcaggt tgaggctgtc ggcgaggtcg gcgggcatcg gcgcacagat
      841 cagacccgac gtccagcgca ccatgaaagc catcacctcg gggctcgcaa gctccgcgga
      901 gatgatgatg tcgccctcgt tctcgcggtt ctcatcgtcg gcgacgatca cgggacggcc
      961 ggcgcgcagc gcgtcgaggg cggcgtcgat ggtggaaagg ctcatcggga gcctccttcc
     1021 gggagagtgc ggaacgcgag caggcgctcg acgtgacggg cgaggatgtc ggtctcgagg
     1081 ttgacgacgt cgccgggagc gagtgccccg aggatcgtcg cctcgagggt ctcggggatg
     1141 agggagacct cgaaccacgc ggacggctcg tcgacgtcgc tgacggcgct caccgtgagc
     1201 gaggtgcccg cgacgctgat cgagcccttg tcgacgacga gcggggcgag gtcggcgggc
     1261 agcgagatgc gcagcacgcg ccactggtcg ccggggcgca cctccagcac ggcgccggtg
     1321 ccgtcgacgt gcccctgcac gatgtggccg cccaggcgcg caccgaccgg catggccttc
     1381 tcgatgttca cgcgggagcc gacgttgagc gcaccgaggg cggagacgtc gagggtctgc
     1441 ttcatgacat cggcgtcgaa ggtgtcggct gtggagccga ccacggtcag gcacacgccg
     1501 ctcacggcga tcgattcgcc gtgcactgca tcggatgccg ccttcggggc acgcacggtc
     1561 agacgccagc cgtctcccga cggcgcgatc ccggcgatct cgccgatctc ctcgatgatt
     1621 ccggtgaaca tcagcttctc tccttcagaa cggaagggcg ggcgaccgcg agcaggtcgt
     1681 cgccgagtgg cagccattcg tcgaccgtga ggcgacgggc atccgcgatc gtgtcgacgc
     1741 cgatgtcggt gagcgcgagg cgcccgccgc cgagcagcac cggggcgagg tacgccagca
     1801 cggtgtccgc gagtcctgcg gcgatgaacg cgctggccag cgtcggtccg
//
LOCUS       DATMSX010000559         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_150413,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000559 DATMSX010000000
VERSION     DATMSX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150413"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1656
                     /locus_tag="VN041_17680"
     CDS             238..1656
                     /locus_tag="VN041_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU30914.1"
                     /translation="MAVTDSLTTQQTPPDDPEYAANLRRATLASSIGSALEYFDFALY
                     GLSTALIFNVLFFPQGDPAMATVAAFATFGVGFLARPFGGLFFGVLGDKIGRKWVLVV
                     TILLMGGASTAIGLLPTYDAVGLWAPILLVLMRLLQGFGAGAEQAGATVLMAEYAPVR
                     RRGFFSALPFIGIQAGTLLAALVFSFISLLPKDALLSWGWRVPFLASFVLILLALFIR
                     MRLRETPTFIDLEKHEQIADRPIREIFTHGLPGVIVGIGLRMAENGGSYMFQTLGLAF
                     FVSVVGDAADKSLLTWGVTIGSLIGMFSVPFAGHLSDRFGRRTIYRFGAVFMLIYCFP
                     AWWMLSLGNHILAIAVIAVGIGVAVNTMLGPQCAMLPELFGNRHRYLGVAMAREISAV
                     LAGGLAGVLGAYLIAVSGGNWILLGTYMGVLALITTASTFLAPETLRRDLTRVDDAIK
                     VSRAEGGDDVFATTVSITAVGR"
     gene            1656..>1754
                     /locus_tag="VN041_17685"
     CDS             1656..>1754
                     /locus_tag="VN041_17685"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775094.1"
                     /note="Involved in the nonphosphorylative, ketogenic
                     oxidation of glucose and oxidizes gluconate to
                     5-ketogluconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HWU30915.1"
                     /translation="MARGVAAFDLTGRTALVSGSSQGIGRALAEGLA"
BASE COUNT          230 a          624 c          578 g          322 t
ORIGIN      
        1 atccgggccg ccgaggcgga cttcgagcgc atcgaacgcg tcggcgaggc tctgcgcgaa
       61 gcagcgcagg cgctgacgaa cccgttcagc gcggctgcgc gccgctgagc tcggccatcc
      121 gggcgtcgcg tcggcgacgt cggagttcgt gcgcccgttg tgagtccaaa gtattgaaca
      181 tcgtccaaca ggctggtaac gtcagtgcca cgccgatggc gaagccgatc gaaggagatg
      241 gcagtgaccg attcactcac cacgcagcag accccgcccg atgaccccga gtacgcggcc
      301 aacctgcgcc gcgcgaccct cgcgtccagc atcggcagcg cactggagta cttcgacttc
      361 gcgctctacg ggctctccac agcgctgatc ttcaacgtgc tgttcttccc gcagggcgac
      421 ccggccatgg cgacggtcgc agccttcgcc accttcgggg tcggcttcct cgcgcgtccg
      481 ttcggcggac tgttcttcgg cgtgctcggc gacaagatcg gccgcaagtg ggtcctggtc
      541 gtcaccatcc tgctgatggg cggcgcctcc accgcgatcg gcctgcttcc cacgtacgac
      601 gcggtcggcc tctgggcgcc gatcctgctc gtgctcatgc gcctgctgca gggcttcggc
      661 gccggagccg agcaggccgg tgcgaccgtg ctgatggccg agtacgcgcc ggtgcgccgc
      721 cgcggcttct tctcggcgct gcccttcatc ggcatccagg ccggcacact gctcgccgcc
      781 ctcgtcttca gcttcatcag cctgctgccg aaggacgcgc tgctcagctg gggctggcgc
      841 gtgccgttcc tggcatcgtt cgtgctgatc ctgctcgcgc tgttcatccg gatgcgcctc
      901 cgcgagacgc cgacgttcat cgatctggag aagcacgagc agatcgccga ccgcccgatc
      961 cgcgagatct tcacgcacgg tcttcccggc gtgatcgtcg gcatcggcct gcgcatggcc
     1021 gagaacggcg gctcctacat gttccagacg ctggggctcg ccttcttcgt gtccgtcgtc
     1081 ggcgacgctg ccgacaagag cctgctgacc tggggcgtga cgatcggctc gctgatcggc
     1141 atgttctccg ttccgttcgc cggtcacctc tccgatcgct tcggacgccg cacgatctat
     1201 cgcttcggcg ccgtgttcat gctgatctac tgcttccccg cctggtggat gctgtcgctg
     1261 ggcaaccaca tcctcgcgat cgccgtgatc gcggtcggca tcggtgtggc ggtgaacacc
     1321 atgctcggcc cgcagtgcgc gatgctgccc gagctgttcg gcaaccgtca ccgctacctg
     1381 ggcgtggcga tggcgcgcga gatctcggcc gtgctggccg gtggcctcgc gggtgtgctg
     1441 ggtgcctacc tcatcgccgt cagcggcgga aactggatcc tgctgggcac ctacatgggc
     1501 gtgctcgcgc tgatcaccac tgcgtcgacg ttcctcgccc cagagacgct gcgccgcgac
     1561 ctgacccgtg tggacgacgc gatcaaggtg tcgcgtgccg agggcggcga cgacgtgttc
     1621 gccacgaccg tctcgatcac ggcggtcgga cggtgatcgc ccgcggcgtc gcggccttcg
     1681 acctgaccgg acggaccgcg ctggtctccg gatccagcca gggcatcggc cgggcgctgg
     1741 ccgaggggct cgcc
//
LOCUS       DATMSX010000560        17997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_141316,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000560 DATMSX010000000
VERSION     DATMSX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 17997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17997
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141316"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VN041_17690"
     CDS             <1..373
                     /locus_tag="VN041_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWU30916.1"
                     /translation="NFGAEHVRRASERLAAAGLTDRLIVDASHGNSGKDHIRQSEVVA
                     ELSDQIATDGRAVAGVMLESNLVAGAQKHDVRNGAAGLVRGQSITDACMGWEATVDAL
                     ATLAAASAARTAARSGMPVTS"
     gene            422..1000
                     /locus_tag="VN041_17695"
     CDS             422..1000
                     /locus_tag="VN041_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU30917.1"
                     /translation="MIITPPARDGASGHVAEMYEEDISGDGFIHTYTAAMAVNPEAHE
                     AFMALVRAVVPSIGVRMYEAATLGAARGIGSAHCLIAHARKSLKAGVVDEAGLRGFAE
                     DDDSGFDAAEQAVIRYAEKLSTAPASMTDTDSQALRAVGFTDRQILDITLAAGLRNHF
                     SRSLLALAVPLDDDLQLDAEVAAALMRRAGRV"
     gene            complement(1022..1171)
                     /locus_tag="VN041_17700"
     CDS             complement(1022..1171)
                     /locus_tag="VN041_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30918.1"
                     /translation="MADQQTDDTTVTTLAAPPSLQLLHTDADAGLCTDGYCVLPGSQA
                     PRPAE"
     gene            1281..1754
                     /locus_tag="VN041_17705"
     CDS             1281..1754
                     /locus_tag="VN041_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HWU30919.1"
                     /translation="MEAAQQRPGETPLERADRNWNELMQELRVMQTGTQILTGFLLAV
                     AFQPRFADLDAVQRTLYVVLVLLAATATVLALAPVGMHRLLFHQHRKIALVRTANRLV
                     KANLVVIGAVTVGVVALILDVTLNRPSAIVASALGVIAILLLWVGLPAMLKRGQD"
     gene            complement(1780..2424)
                     /locus_tag="VN041_17710"
     CDS             complement(1780..2424)
                     /locus_tag="VN041_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWU30920.1"
                     /translation="MDDQAVRASVRVRTSVSVVIGILAGAGIGIWLGPAAGILGGWGT
                     LALVNAVWVLLIVWPMDAAATRAHALAEDPGRRVARIVAIIGSVVSLAAVGVVVVQAR
                     HAPEIQSYLLAGTAVLSVVASWVLIQADYMLRYARMYYSEPAGGISFNQHDDPQYSDF
                     AYFAVGLGMTYQVADTNVSNGAIRRVVVAQTMLAYLFGAVILATVINLVTSLGS"
     gene            2569..2859
                     /locus_tag="VN041_17715"
     CDS             2569..2859
                     /locus_tag="VN041_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HWU30921.1"
                     /translation="MLTLTENANTIAATIVAQQSTTPDAGLRIHSSGTEAEPRFAVTV
                     APTPEPGDEVIGDDEIHVFLEHAAAEALNDKVLDATVDEQGAVSFTLLPQAA"
     gene            2948..3526
                     /locus_tag="VN041_17720"
     CDS             2948..3526
                     /locus_tag="VN041_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWU30922.1"
                     /translation="MLRRLISRVFWTFSRWTLTTEPAPSRPTVLIGAPHTSNWDFVLM
                     LAIAWRLGIDVRWLGKKSLFEGWRGPIMRALGGIPVDRSDPGRVVGDVVAKVQDGEVF
                     GLVVTPDGTRKGHSHWKSGFYRIARETGMPVTLGYVDRTTMTTGLGPTIELTGDVAAD
                     MDRIRAFYADKSGLRPEHRVEPRLREEQPFTS"
     gene            complement(3516..4613)
                     /locus_tag="VN041_17725"
     CDS             complement(3516..4613)
                     /locus_tag="VN041_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HWU30923.1"
                     /translation="MSAKTDLGAEPLSPIRHAVADLSPGYFAAVMGTGIVSIGLHAVG
                     IGPLSAVLMWIAAILYVILWTLYIWRAVRFWHLVVADLRDPEVAFAYFTVVAATDVLG
                     VRVALEGRTVVAGWLFLCAALIWFVFGYVLPWQVLMIRDGKTILGHANGTWFVWAVAS
                     QSLAIGMAQLRPQVDTAIAQWIGLLAVLAWSVGVILYAAMAMLVLLRVVHFGLAPQQF
                     DPAYWVAMGALAIAVVAGAGIVQMEHTPLVDAVRPLIAATLVIFWVFCLWLIPLLIGA
                     GIWRHALHRVPLRYVPTLWSMVFPMGMFAVASLSLNEADALPAAGAVGGVALVVAVIV
                     WAVVLAGMLHQGLNVLWATLRRGATAQRLTR"
     gene            complement(4680..5006)
                     /locus_tag="VN041_17730"
     CDS             complement(4680..5006)
                     /locus_tag="VN041_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HWU30924.1"
                     /translation="MTGQEEPATAEELMRSRFAAFKSADAACLLRTWHPSTRPDDLDL
                     GDNPRWRGLQIVDVVDGGADAQSGVVEFRATYLQDDGELGILHERSRFVREGGRWYYV
                     DGDLFE"
     gene            complement(5014..5553)
                     /locus_tag="VN041_17735"
     CDS             complement(5014..5553)
                     /locus_tag="VN041_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU30925.1"
                     /translation="MAESSTFEPDGRAVDYVDEGEGPVGLVLIHGIGRTGEALGVIAH
                     YLAEEAGFRVVQIGADAVDDVIAVMDHLGLEDAWIGGHGSGGTLARHVVAAHHERSNG
                     LLLLGVEDEDIALAPALPVLIIQGAEDDVTPPANGDRLQATAPERASVKTLAGADHHF
                     PVTHPIETAYIIEEYLDWD"
     gene            complement(5671..6537)
                     /locus_tag="VN041_17740"
     CDS             complement(5671..6537)
                     /locus_tag="VN041_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWU30926.1"
                     /translation="MIEVLNHLVDEIEAHLGDETDVDALAARLGTTGYHARRMFSSLA
                     GMPVSEYVRRRRMTVAASDLIGGDDLLTIAVRYGYGSTEAFGRAFRAVHGASHGDVRR
                     DGGPLRSQPQLRFRLTVEGSTPMDARITERPAFRLIGHAARVPLIHEGINPHIQAHIA
                     SLPIEEHARLKQLSDTEPAGLLQVSADVDPDYAQGSELTYLHGVAITADTDVPADLDA
                     IDVPAGAWAVFRTSGAYPAALQSTWAATATEWFPSNRWRLRPGPSIVAVLDRADDFST
                     ATTELWLPIERS"
     gene            complement(6616..6978)
                     /locus_tag="VN041_17745"
     CDS             complement(6616..6978)
                     /locus_tag="VN041_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30927.1"
                     /translation="MGRRKTPEERADEERRYALASAAHTDEDFEPFFTDTNQAIRNAA
                     AMNPDASAAVLDRFASDRFWSVRFAVAEHPRTDRATLLRMLEADPRRRGVVHHETRKR
                     LEREGVRFGEDGMPVPED"
     gene            complement(7024..7758)
                     /locus_tag="VN041_17750"
     CDS             complement(7024..7758)
                     /locus_tag="VN041_17750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30928.1"
                     /translation="MSALQDYDARIVDLYDLDNPDGPDHDFYRALADSRDARSILDLG
                     CGTGILTVTLAAEGRRVVGVDPSATMLAFARTRVGGEQVEWVEGDTRMLPEGRFDLML
                     MTGNVAQHIPDPAWMLTLRHLRAVAAPGAMLAFESRNPAARAWESWTREEPSVRETMH
                     GALTEWSEIEERGGGVVLLRAFNRFERTGETVVEEQVLTFRTEERIIQDLQTAGFDTA
                     AVWGDWARAPFDGRQRLMIFEATPTA"
     gene            7912..8568
                     /locus_tag="VN041_17755"
     CDS             7912..8568
                     /locus_tag="VN041_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU30929.1"
                     /translation="MSQVRVHNFSVSLDGFGTGDGITVDAPFGHAGERLHEWMFATRF
                     WRSMVGEPGGSTGIDNVFAEQHGLGFGAEIMGRGKFSASTGPWTDIGTDDEWRGWWGP
                     NPPFHTPVFVLTHHARPSIEMEGGTVFHFVDASPAEALDLAREAAGDLDVRIGGGPAL
                     LREFLAAGLIDVAHVVQVPILLGRGVRLWDGLDALDERYAIEAVSSPSGVTHLTLTRR
                     "
     gene            8586..9029
                     /gene="arfB"
                     /locus_tag="VN041_17760"
     CDS             8586..9029
                     /gene="arfB"
                     /locus_tag="VN041_17760"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWU30930.1"
                     /translation="MDDLHVLPGPGAPRGIVVPAADLVEQFSRASGPGGQGVNTTDSR
                     VQLMIDLGTTSALDETQRRRVLVRLAPRLTGTTLIISASEQRSQRLNRIAARERLAAL
                     LRDAVVPAVARRATRPTRSSQRRRLDEKKRRSETKDARRRPGAAD"
     gene            complement(9053..9874)
                     /locus_tag="VN041_17765"
     CDS             complement(9053..9874)
                     /locus_tag="VN041_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWU30931.1"
                     /translation="MPESPAVQALVEFLDERITGREITEVDVVEFRIIKTRDRLPASL
                     IGARINGLRRFGKHAGLETTAGWLMIGFGRNGWIRWQAPGDPALDPRGPEVARIILDD
                     GAVMQLVDTGDFLAVTASVVDAPTDVAGIAALGPDPLSADFTADHLERALGTRRKQIK
                     ALLQEQTSLAGIGNAYSDEILHRARIPLATHAALLGTADRERLFTAVVETMTTAARER
                     RGLRPDELKQAKVSAMAVHGRGGEACPVCGATIVDQTFGGASAQSCPQCQPEHDN"
     gene            complement(10015..10485)
                     /locus_tag="VN041_17770"
     CDS             complement(10015..10485)
                     /locus_tag="VN041_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWU30932.1"
                     /translation="MTEGEKTRLVAWSEELRSVHARLRKALRIAQDAAAEGGSVETAS
                     RDLLLFCHGFCAALTGHHVAEDRELFPAIAAEHPHLRETLRKLEQDHSMIAHLMGGLQ
                     AAVDSRAAPDELARHLDGVSAIMESHFRYEERQLLTVLETLALDAEPSRVLGPL"
     gene            complement(10689..10859)
                     /locus_tag="VN041_17775"
     CDS             complement(10689..10859)
                     /locus_tag="VN041_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30933.1"
                     /translation="MGQFDQLPGETREHFVRRFSQRPGVVADGVTLVDLYNRIRMLEA
                     RVDELEERDAEG"
     gene            complement(10941..12014)
                     /gene="ychF"
                     /locus_tag="VN041_17780"
     CDS             complement(10941..12014)
                     /gene="ychF"
                     /locus_tag="VN041_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWU30934.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNDVLAANYPFATIEPNVGVV
                     SLPDKRLDVLAGIFGSERILPAAVSFVDIAGIVRGASEGEGLGNQFLANIREADAIAQ
                     VVRGFADDDVVHVEGAVNPASDMETINAELMLADLQTLEKAIVRLEKEVKGKKTEPVV
                     LEMAKAAQDALERGILLSVSGLDLAPIKELGLLTAKPVIFVFNVDESVLTDAARKAEL
                     EALVAPAKAIFLDAKIESELIDLDPEDAAELLASTGQEESGLDQLARIGFDTLGLQTY
                     LTAGPKEARAWTIHKGDKAPQAAGVIHTDFEKGFIKAEVISFEDLVETGSVAEARAKG
                     KARLEGKDYVMQDGDVVEFRFNV"
     gene            complement(12040..12699)
                     /locus_tag="VN041_17785"
     CDS             complement(12040..12699)
                     /locus_tag="VN041_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HWU30935.1"
                     /translation="MRLLLTSGGVTNPSIRTALVEMLGKPIEDSTALFIPTAQYGHPS
                     CTPEVTLRSASSDWSMTALGWKSAGLLELTALPSMEGERWVSWVEQADALLVDGGDAT
                     YLAHWIRRSGLFKLFPRLERTVWVGVSAGSMVMTPRIGAEFERWTPPEPGEGTLGIVD
                     FSLFPHLDYPDFPENTLAHAERWAEHIDGPAYAIDDQTAIRVVGEDVDVISEGHWKRL
                     E"
     gene            12815..13351
                     /locus_tag="VN041_17790"
     CDS             12815..13351
                     /locus_tag="VN041_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30936.1"
                     /translation="MPDDVNADEITDPFWSIVRRRHPDLDIVLLPPAKPAPDTSGLEP
                     QAPETFAHEHLAEVDRAWADLVGHGMPLSTAQWIPGPTRDSVCHSVTLTLGEVEESIA
                     LGHLRAAAELLAPGHWRVFLPPRGMPRVTADRDGRLGDEHLLFGYAIENRNLFLRLES
                     TGVPVGERTAYELIGAAA"
     gene            13348..13650
                     /locus_tag="VN041_17795"
     CDS             13348..13650
                     /locus_tag="VN041_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30937.1"
                     /translation="MTDGVEFNPALLFTVIPQWRTQASQMRSAASSLGGAPTGGLPAS
                     AAGAAKTFLSMWENTTRKGSVAADVYADELESTGTSYQNIDAEIARRMDAMGAGAR"
     gene            13647..15419
                     /locus_tag="VN041_17800"
     CDS             13647..15419
                     /locus_tag="VN041_17800"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU30938.1"
                     /translation="MTTVQIPAPLHYPDRPDGDPAAAETLAGTLLHGSAVTTEFADVA
                     ALLSDPGYMWRGAASEAYTAHSARFAKQYEPMGETLKRVARGVDVFAAELRELQSEHT
                     TLVGQVTAYDRDRVALIADVDAGTEADVPALQDRARRLSTQRTGIADDITDFQKRTTD
                     NEQYLTRLFTGADTEAEAQAPGGGVDDMATLAILTMPLFKDPKAMANWWNGLSEAEQN
                     AVIAAYPEKIGSADGLPASARDGANRLLLESDLAELHQKEADGTLTDQEKQRLKNAEA
                     TQKALKYSDDYVVPGTNDHPGGQLWLYDPDAFNGDGRVAIALGDLDTADDVSVSVPGI
                     QTQMDGVAGNMKDTAHLYESARHSGDGSSVATMFWLGYDTPAGDLLEWGEVPSEDRAT
                     DGGARLADAVDGLRASRPDNPAHMTAIGHSYGSTTTSYAATNHHLAVDDIALIGSPGT
                     GPADHASDFSVGADHVYDGRNSRDLVAALGEQGWLRKSQLLGLGLGVDPSSEDFGAHR
                     FEAESVNRADHIRNIDDHTRYYDRDSESLYNLGRIVDGHGDDVVGADQSYDPWWGPPQ
                     DPEIDRTPTTDVPGRSDTGFGNEP"
     gene            15419..15970
                     /locus_tag="VN041_17805"
     CDS             15419..15970
                     /locus_tag="VN041_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30939.1"
                     /translation="MRKAVMMGVVGLLAFLLGGCSSVPSDPRAVLEEKAATIDAAVQD
                     LFETLAVAGLHHVSAASAVDTCQSEPAPGVSYRAGMTVKAGEDLAGGFDALVKQLDAA
                     GWKSTDAYRDVKIDPARPMGRFVRDDITLDVKTGGASSGGVWYGADEMQLGVTIDDDC
                     VRVPDGGYISKVEDLGKDILPRS"
     gene            complement(15992..16702)
                     /locus_tag="VN041_17810"
     CDS             complement(15992..16702)
                     /locus_tag="VN041_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWU30940.1"
                     /translation="MTESIRAVFFDLDDTLVGDGAVDAALLAIDELAARHPDLDTDAM
                     RTANATVWSGCWASQGEQWMRGELEDDALTREVWRRTLSGAGAGGELVDEAVELHLEA
                     ERRTFALFEDSRDVLVELRRRGIPIGLITNGPSAFQREKLRTVGIDGLFDVVVASGDI
                     GVLKPDAEIFRHALDRAGVLSAASLHVGDSFPADVMGAVGAGLSAFWLNRERIAAPPN
                     GPRHVEGDSLRAVLKLVG"
     gene            complement(16695..17438)
                     /locus_tag="VN041_17815"
     CDS             complement(16695..17438)
                     /locus_tag="VN041_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30941.1"
                     /translation="MSDVRATSFGSEADTYEAARPDYPFEAVAWMLEKLPHEARRIAD
                     VGAGTGKLTRVIADAPDAQVVAVDPDAAMLAKLRDTVPGVPTYVGTAESLPLPDASLD
                     AVVLGQAWHWVDPETASAEIGRVVRPGGTLGLIWNARDNRVDWVRRFTAIMHASAAEE
                     MLDAGGPTVAAPFGELEAERWEWVRPMTRAQLHRMADSRSYLITAAEQERASIHEGMD
                     ALFDELGVTGEDTIDLPYVTTAFRARRRD"
     gene            17499..>17997
                     /locus_tag="VN041_17820"
     CDS             17499..>17997
                     /locus_tag="VN041_17820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWU30942.1"
                     /translation="MPLDFTAIDFETANSSPASACSVGLVRVRDGKVVAQTGWLIRPP
                     AGHDEFNEWNTRIHGLYAHDVVNAATWVQQIGSLCAFAGADVLVAHNAGFDMNVLRRA
                     CEATGIDAPPYRSLCSLAVARKTYDLDSYRLPKAAAAAGFGDFPHHDALADARACAQI
                     VIDAAR"
BASE COUNT         2818 a         6404 c         6090 g         2685 t
ORIGIN      
        1 gaacttcggc gccgagcatg tgcggcgggc gtccgagcgg ctcgccgccg cgggactcac
       61 cgaccggctg atcgtcgatg ccagccacgg caacagcggc aaggaccaca tccggcagtc
      121 ggaggtggtc gcggagctct cggatcagat cgcgaccgac ggccgcgcgg tggccggcgt
      181 catgctcgag agcaacctgg tcgcgggcgc gcagaagcac gacgtccgga acggcgcggc
      241 gggtctggtg cgcggtcaga gcatcaccga cgcgtgcatg ggatgggagg ccaccgtgga
      301 cgcactcgcc accctcgccg ccgcgagcgc agcccgcacc gccgcgcgtt ccgggatgcc
      361 tgtgacgtcc tgacgatccg aggcttcccc gcacggcgac agcggagtac tgtgcggtgc
      421 catgatcatc actcccccgg cgcgggacgg cgccagcggt cacgtcgccg agatgtatga
      481 ggaggacatc tccggcgacg gcttcatcca cacgtacacc gccgcgatgg cggtgaatcc
      541 ggaggcgcac gaggcgttca tggcgctcgt gcgagcagtc gtcccgtcca tcggcgtccg
      601 catgtacgag gcagcgaccc tgggggcggc gcgtggcatc gggtcggcgc actgcctgat
      661 cgcccacgcg cgcaaatcgc tgaaggcggg ggtcgtagac gaggccgggc tgcgcggttt
      721 cgccgaggac gacgacagcg ggttcgacgc ggctgagcag gcggtgatcc gctatgccga
      781 gaagctctcg accgcgccgg cctcgatgac ggataccgac tcacaggccc tgcgggccgt
      841 gggattcacg gatcggcaga tcctggacat caccctggcc gcggggctgc gcaatcactt
      901 cagccggtcg ctgctcgccc tggccgtgcc tctggatgac gacctccagc tggacgcgga
      961 ggtcgcggcc gcgctgatgc ggcgcgccgg gcgggtctga gcgcagcgac tcgacgggag
     1021 gtcactccgc gggtcggggc gcctggctgc cgggcagcac gcagtagccg tcggtgcaca
     1081 gccccgcgtc ggcgtccgtg tgaagcagct gcagggacgg aggcgcagcg agcgtcgtca
     1141 ccgtcgtgtc atctgtctgc tgatcagcca tccttcgatg gtacgccgcc ccgccgggac
     1201 gggctccggc accggccgag ctacgcatcg ttgcgcgcgc cccgtcgctc tcccgttcgg
     1261 tcacgagtag cctgccgctc atggaagcag cgcagcagcg ccccggcgag acgccgctcg
     1321 aacgggccga ccgcaactgg aacgagctca tgcaggaact gcgcgtcatg cagaccggca
     1381 cgcagatcct caccggcttc ctgctggcgg tcgccttcca gccgcggttc gcagacctgg
     1441 atgcggtgca gcggacgctg tacgtggtgc tcgtgctgct ggctgcgact gcgaccgtgc
     1501 tggcgctggc gccggtcggc atgcatcgcc tgctgttcca ccagcaccgc aagatcgccc
     1561 tggtgcggac ggcgaaccgg ctggtcaagg cgaacctcgt ggtcatcggc gcggtgacgg
     1621 tcggcgtggt ggcgctgatc ctcgacgtca cgctgaaccg gcccagcgcg atcgtggcat
     1681 cggcactcgg cgtgatcgcg atcctgctgc tgtgggtggg actgcctgcg atgctcaagc
     1741 gcggccagga ctgacgcccc ggcgccaccg ccccggcgct cagctgccga ggcttgtgac
     1801 gagattgatg acggtggcga ggatcaccgc gccgaacaga taggcgagca tcgtctgagc
     1861 cacgacgacc cgcctgatcg ccccgttcga cacgttcgtg tcggcgacct gataggtcat
     1921 gccgagcccg accgcgaagt aggcgaagtc gctgtactgc gggtcgtcgt gctggttgaa
     1981 cgagatgccc ccggcgggct cggagtagta catccgggcg tagcgcagca tgtagtcggc
     2041 ctggatcagc acccacgagg cgacgacgct gagcaccgcg gttccggcga gcagatagct
     2101 ctggatctca ggcgcgtgcc gtgcctgcac gacgaccacg ccgacggcgg cgaggctgac
     2161 gacgctgccg atgatggcga cgatccgcgc cacgcggcgc cccgggtcct ccgccagcgc
     2221 gtgagcccgg gtcgcggcgg catccatcgg ccacacgatc agcagcaccc agaccgcgtt
     2281 gaccagtgcg agggtgcccc agccgcccag gatgcctgcg gcggggccga gccagatgcc
     2341 gatgccggcg ccggccagga tgccgatcac gaccgagacg gaggtcctca ctcgcacgga
     2401 ggcgcgaacg gcttgatcgt ccatgagcga atgctacgcg ccggatccgg ctcagatgtg
     2461 aatggcctcc gagtcgggcg gttcgggttg tccgtcgacg cctggtgcgg aatcgtggct
     2521 cggacaccgt gctggacggt gcggtgtccg caaccggaag gagagctcat gctcaccctg
     2581 accgagaacg caaacacgat cgcggcgacc atcgtcgccc agcagagcac gacgccggat
     2641 gcgggccttc gcatccactc gtcggggacg gaggcggagc cgcgcttcgc cgtcacggtc
     2701 gctccgacgc ccgagcccgg tgacgaggtc atcggggacg acgagatcca cgtgttcctc
     2761 gagcacgccg ccgccgaggc tctgaacgac aaggtgctcg acgccacggt cgacgagcag
     2821 ggtgcggtgt cgttcacact gctcccgcag gcggcttgac cgtccgcacc acgaagagcc
     2881 gtcgccttcc cgggcggcgg ctcttcgtat gccgcgtggc gcatgtgccc ggctctagcg
     2941 tggaatcatg ctccgccgcc tgatctcccg ggtgttctgg acgttcagcc gctggacgct
     3001 gacgaccgaa cccgccccct ctcgaccgac ggtgctgatc ggcgctcccc acacctccaa
     3061 ctgggacttc gtgctgatgc tcgcgatcgc ctggcggctg gggatcgacg tgcggtggct
     3121 cgggaagaag agcctgttcg aggggtggcg aggtccgatc atgcgcgcac tcggcggcat
     3181 cccggtggac cgcagcgatc ccgggcgcgt ggtcggcgac gtggtcgcga aggtgcagga
     3241 cggcgaggtc ttcggcctgg tcgtcactcc ggacggcaca cgcaaggggc actcgcactg
     3301 gaagtccggc ttctaccgca tcgcgcgcga gaccgggatg ccggtgaccc tcggctatgt
     3361 cgaccgcacc acgatgacga cgggactcgg gccgaccatc gaactgaccg gcgatgtggc
     3421 cgccgacatg gaccgcatcc gcgccttcta cgccgacaag tcgggcctgc ggcccgagca
     3481 ccgcgtcgag ccgcgactgc gcgaggagca gccgttcacc tcgtgagccg ctgcgctgtg
     3541 gcgcctctcc tcagggtcgc ccacagcaca ttcagcccct ggtgcagcat cccggccagc
     3601 accactgccc agacgatcac cgcgacgacg agcgcgaccc cgccgaccgc tcccgccgcc
     3661 ggtagcgcgt ccgcctcgtt cagggacagc gatgcgacgg cgaacatgcc catcgggaac
     3721 accatcgacc acagtgtcgg cacgtagcgc agcggcaccc ggtgcagcgc gtgccgccag
     3781 atgccggcgc cgatcagcag cggtatgagc cacaggcaga acacccagaa gatcacgagg
     3841 gtcgccgcga tgagcggccg gaccgcgtcg acgagcggcg tatgctccat ctgcacgatt
     3901 ccggcgccag cgaccacagc gatcgcgaga gcccccatgg caacccagta ggcggggtcg
     3961 aactgctgcg gtgcgagacc gaaatgcacc accctcagca gaacgagcat cgccatcgcc
     4021 gcgtagagga tcacccccac agaccaggcg agcacggcga gcagcccgat ccactgcgcg
     4081 atggcggtgt ccacctgcgg acgcagctgc gccatcccga tcgccagcga ctggctggcg
     4141 accgcccaga cgaaccacgt gccgttggcg tggccgagga tggtctttcc gtccctgatc
     4201 atcagcacct gccacggcag cacgtagccg aacacgaacc aaatcagcgc ggcgcacagg
     4261 aacagccacc cggcgacgac ggtgcggccc tcgagtgcga cgcgcacccc gagcacatcg
     4321 gtcgccgcga ccacggtgaa gtaggcaaac gcgacctcgg gatcgcgcag gtcggcgacg
     4381 acgaggtgcc agaaccggac cgccctccag atgtagagcg tccagaggat gacgtacagg
     4441 atcgcggcga tccacatgag caccgcggag agcggcccga tgccgacggc gtgcagtccg
     4501 atcgagacga tgccggtgcc catcaccgcg gcgaagtagc cgggggacag atcggcgacc
     4561 gcgtggcgga tcggcgacag gggttccgcc ccgagatccg tcttcgcgct catctcgttc
     4621 ctgctcccgg tcatcggcgg cgtatgccgc cttctccagt atcccgcgca gctgccgcgc
     4681 tactcgaaca ggtcgccgtc cacgtaatac cagcgaccgc cctcacggac gaagcgcgag
     4741 cgctcgtgca ggatgcccag ctcgccgtcg tcctgcaggt aggtggcgcg gaactcgacg
     4801 acgccggatt gggcatccgc tcccccgtcc acgacgtcga cgatctgcag cccgcgccag
     4861 cgcgggttgt cgccgaggtc gagatcgtcc ggccgggtgg acggatgcca ggtgcgcagc
     4921 aggcacgccg catccgcact cttgaacgcg gcgaaccgcg agcgcatcag ttcctcagcg
     4981 gtggcaggct cctcctggcc ggtcatgacg cggtcagtcc cagtccaggt actcctcgat
     5041 gatgtacgcg gtctcgatgg ggtgcgtcac cgggaagtgg tggtcggcgc cggcgagagt
     5101 cttcacgctc gcgcgctccg gcgcagtggc ctgcaggcgg tcgccgttcg ccggcggggt
     5161 gacgtcgtct tccgcgccct ggatgatgag cacgggcagt gccggggcga gcgcgatgtc
     5221 ctcgtcctcc acgcccagca gcagcaggcc gttggagcgc tcgtggtgcg ccgcgacgac
     5281 gtggcgcgcg agcgtcccac ccgagccgtg accgccgatc caggcatcct ccaggcccag
     5341 gtgatccatc accgcgatga cgtcgtcgac ggcatccgcg ccgatctgca cgacacggaa
     5401 gcccgcctcc tcggcgaggt agtgcgcgat cacgccgagc gcctcacctg tgcggccgat
     5461 gccgtggatg agcacgagac cgacgggtcc ctcgccctcg tcgacgtagt cgacggcacg
     5521 gccgtccggc tcgaaggtgc tgctctctgc cactggatgc tcctcagggt cgtgaacggg
     5581 ttgcgcccca tcaaccctac ttggccaccc cgcgacggtg cggccgcgta cgcccggcat
     5641 ccggtgcatc cgcggccgca cggcctcgat tcagctccgc tcgatcggca gccacagctc
     5701 cgtcgtcgcg gtgctgaagt cgtcggcgcg gtcgagcacg gcgacgatcg acggccccgg
     5761 acgcagacgc cagcggttgg acgggaacca ctcggtcgcg gtcgcggccc aggtggactg
     5821 cagtgcggcc ggatatgcgc cagaggtgcg gaacaccgcc cacgcccccg cgggcacgtc
     5881 gatggcgtcc agatccgccg gcacgtcggt gtcggcggtg atcgcgactc cgtgcaggta
     5941 cgtcagctcg ctcccctgcg cgtagtcggg gtcgacgtcg gcgctgacct gcaggagccc
     6001 ggcgggctcg gtgtcgctca gctgcttgag gcgcgcgtgc tcctcgatcg gcagcgaggc
     6061 gatgtgcgcc tggatatgcg gattgatccc ctcgtggatc agcggcacgc gcgcggcgtg
     6121 tccgatcagg cggaaggcgg gtcgttcggt gatgcgggca tccatggggg tgctcccttc
     6181 tacggtcagg cggaacctga gctgcggttg actgcgaagg gggccgccgt cacgccggac
     6241 atcaccgtgg ctcgcaccgt gcacggcgcg gaacgcccgg ccgaaggcct cggtcgagcc
     6301 gtatccatag cgaacagcga tggtcagcag atcgtcgccg ccgatcaggt cggatgccgc
     6361 gaccgtcatc cgacgccgtc gcacgtactc cgacacgggc atgcccgcga gcgacgagaa
     6421 catccgcctg gcgtgatacc cggtggtgcc caggcgcgcg gccagcgcgt ccacgtccgt
     6481 ctcgtcgccg agatgcgctt cgatctcgtc gaccaggtgg ttgagcactt cgatcatggc
     6541 tgctcccttc ggcatccact ctcgtctccg cgaccgtcgc ggcgcccgat catcgcggtc
     6601 cgattcgatc ggatgctaat cctccgggac cggcatcccg tcctccccga atcggacgcc
     6661 ttcgcgctcc aggcgcttcc gcgtctcgtg atgcaccaca ccgcggcggc gcgggtcggc
     6721 ctcgagcatg cgcagcaggg tcgcgcggtc ggtgcgagga tgctcggcga cggcgaatct
     6781 cacgctccag aagcggtccg acgcgaagcg gtcgagcacg gccgcgctgg catccgggtt
     6841 catcgccgcg gcgttccgga tcgcctggtt cgtgtcggtg aagaacggct cgaagtcctc
     6901 gtccgtgtgc gcggcgctcg cgagcgcgta gcggcgctcc tcgtcggccc gctcctccgg
     6961 tgtcttgcgc ctgcccatgc acatccccct cgttctcgcg cccctaccag ggacgattcg
     7021 atgctatgcg gtcggcgtgg cctcgaagat catgagcctc tgccgaccgt cgaacggcgc
     7081 acgcgcccag tctccccaca ccgcggcggt gtcgaagccg gcggtctgca gatcctggat
     7141 gatgcgctcc tcggtgcgga aggtcagcac ctgctcctcg acgaccgtct caccggtgcg
     7201 ctcgaagcga ttgaacgcgc gcagcagcac gacgccgccc ccgcgttcct cgatctcgct
     7261 ccattccgtc agtgccccgt gcatcgtctc cctgacggac ggctcctccc gggtccacga
     7321 ctcccaggcg cgcgccgcgg ggttacggct ctcgaaggcg agcatcgcgc caggagccgc
     7381 gacggcacgc aggtgccgca gcgtgagcat ccacgccggg tcggggatgt gctgggcgac
     7441 gttgcccgtc atcagcatca ggtcgaatcg tccctcgggc agcatccggg tgtcgccctc
     7501 gacccactcc acctgctccc cgcccacgcg agtccgcgcg aaggcgagca tcgtcgcgga
     7561 ggggtcgacg cccacgacgc gccttccctc ggccgccagg gtgacggtga ggatgccggt
     7621 gccgcatccc aggtccagga tgctccgcgc gtcacgggag tcggccagcg cgcggtagaa
     7681 gtcgtgatcc ggcccgtcgg gattgtcgag gtcgtagaga tcgacgatcc gcgcgtcgta
     7741 gtcctgcagg gcactcatcc gaacaggcta gccggatgcc gtcgtctcga acgcccgccc
     7801 cgcgcggcgc tcacgccgag cacggcgccg ccgagagctg cgggaagtcg gagagtagat
     7861 tacccggagc acgattcgca gatcaccgtc gctcaaccgt gggagtcgct catgtcgcag
     7921 gttcgtgtcc acaacttctc cgtctcgctc gacggcttcg gcacaggcga cggcatcacg
     7981 gtcgacgccc cgttcggcca cgccggcgag cgactgcacg agtggatgtt cgccacgcgg
     8041 ttctggcggt ccatggtcgg ggagccgggc ggttccacgg ggatcgacaa cgtgttcgcc
     8101 gaacagcacg gtctcggttt cggcgccgag atcatgggcc gcggcaagtt cagcgcgtcc
     8161 accggcccct ggaccgacat cggcaccgac gacgagtggc gcggctggtg ggggcccaac
     8221 cctcccttcc acacacccgt cttcgtcctc acccaccacg cccgcccctc gatcgagatg
     8281 gagggaggca cggtgttcca cttcgtcgac gcctcaccgg cagaagcgct cgatctggcg
     8341 cgcgaggcgg cgggcgacct ggacgtgcgg atcggcggcg gacccgccct gctgcgagag
     8401 ttcctcgccg caggactcat cgatgtcgcc cacgtggtgc aggtgccgat cctcctcgga
     8461 cgcggcgtcc ggctgtggga cggtctggat gctctcgacg agcgctacgc gatcgaggcc
     8521 gtgtcctccc cgagcggggt gacgcacctg accctcaccc gacggtgatg tgacagcctg
     8581 gacacgtgga cgatctgcac gtgctgccgg gccctggtgc gccccggggc atcgtcgtgc
     8641 ccgctgcgga tctcgtcgag cagttctccc gggcatcagg accgggcggg cagggtgtga
     8701 acaccacgga ttcgcgcgtg cagctcatga tcgatctggg aacgacgagc gccctcgatg
     8761 agacgcagcg tcgccgcgtg ctggtgcggc tcgcaccgcg gctgaccgga acgactctca
     8821 tcatcagcgc gagcgagcag cgctcccagc gattgaatcg catcgccgcg agggaacggc
     8881 tcgccgcgct gctgcgtgat gccgtcgtgc cggccgtggc acgacgggcc acccgcccga
     8941 cgaggagttc ccagcgacgg cgcctcgacg agaagaagcg gcgctccgag acgaaggacg
     9001 cgcggcggcg tcccggtgct gccgactgag cagtcgtgat cggcgcgcgc cgtcagttgt
     9061 cgtgctccgg ctggcactgc gggcacgact gcgcggatgc cccgccgaac gtctggtcga
     9121 cgatcgtggc gccgcatacc ggacacgcct ctccgccccg gccatgcacg gccatggcgg
     9181 agaccttcgc ctgcttgagc tcatcagggc ggagcccgcg gcgctcccgc gcagctgtgg
     9241 tcatggtctc gacgaccgcg gtgaagagcc gctcccggtc cgccgtgccg agaagggcgg
     9301 catgggtcgc gagcggaatc cgtgcccgat gcaggatctc atcggagtac gcgttgccga
     9361 tgcccgcgag tgaggtctgc tcctgcagca gcgccttgat ctgcttgcgg cgggtgccga
     9421 gtgcacgctc gaggtgatcg gcggtgaaat cggcggagag cgggtccggg ccgagcgcgg
     9481 cgatgccggc gacatccgtc ggggcatcca ccaccgatgc cgtcacggcg aggaagtccc
     9541 ccgtgtccac gagctgcatc acggcgccgt cgtcgaggat gatcctggcg acctccggcc
     9601 cgcgcggatc gagagccgga tctccgggcg cctgccatcg tatccagccg ttgcggccga
     9661 atccgatcat gagccagccc gccgtcgtct cgagcccggc gtgcttgccg aatcgccgca
     9721 gcccgttgat gcgggcgccg atcagcgagg cgggaaggcg gtcgcgcgtc ttgatgatgc
     9781 ggaactcgac tacgtccacc tcggtgatct cacgacctgt gatccgctca tcgaggaact
     9841 cgacgagcgc ctggaccgcc ggcgattcgg gcatgcgtac atcctcgcac cgtgccctcg
     9901 tgttcggccc gccggggtcg aggtctcaac cttcacggcc tgggcaggag tcgtggccgg
     9961 gggcgggccc gcttcccggt gcgggcgacg ctcatcgccc gcaccgggaa aacctcagag
    10021 aggtccgagc acgcggctcg gctcggcgtc cagggcgagc gtctccagca cggtgagcag
    10081 ctgccgctcc tcgtagcgga agtggctctc catgatcgcg gacaccccgt cgaggtgccg
    10141 tgcgagctcg tccggcgcag cccggctgtc gaccgccgcc tgcagcccgc ccatcaggtg
    10201 ggcgatcatc gaatgatcct gctcgagctt gcgcagcgtc tcccgcaggt gcggatgctc
    10261 cgcggcgatg gcggggaaca gttcccggtc ctccgccaca tggtgcccgg tcagcgcagc
    10321 gcagaatcca tggcagaaca gaagcagatc acgactcgcc gtctcaaccg atccgccttc
    10381 ggctgcggca tcctgcgcga tccgcagcgc cttgcgcagc cgtgcatgca cgctccgcag
    10441 ctcttcactc caggccacca gcctggtctt ctcgccctca gtcacgctgg ggcgaaggga
    10501 tagacatcgt caacgtccgt ctccttcagg ttccacgcct ccatgcctga cacggtctgc
    10561 caccggcacg cgacgctgaa ggccagcgta gtgcaccgct gcgatggctc cgggttcatt
    10621 gagcgcgttc ctcgacacga cctgcccgca atgccatgag cgcgccagag catcgctcga
    10681 gacgcgagtc agccctcagc gtcccgctcc tcgagttcgt cgaccctggc ctcgagcatc
    10741 cggatccggt tgtagaggtc cacgagcgtg acgccgtcgg cgacgacgcc ggggcgctga
    10801 gagaagcggc ggacgaaatg ctcgcgtgtc tctcccggca gctgatcgaa ctggcccatg
    10861 aatcccctcc gcgtcgagtg tctgttcagg atagcgatcg ctggggagat gcgatgcgca
    10921 gccgcatccg gctgctcgag ctagacgttg aagcggaact ccaccacgtc gccgtcctgc
    10981 atgacgtagt ccttgccctc caggcgcgcc ttgcccttcg cgcgcgcctc ggcgaccgag
    11041 ccggtctcga ccaggtcctc gaacgagatg acctcggcct tgatgaagcc cttctcgaag
    11101 tcggtgtgga tcacgccggc ggcctgcggg gccttgtcgc ccttgtggat cgtccaggcg
    11161 cgggcctcct tcggccccgc ggtcaggtag gtctgcagac caagggtgtc gaacccgatg
    11221 cgtgccagct ggtccaggcc ggactcctcc tgacccgtgg aggcgaggag ctcggcggcg
    11281 tcctccgggt ccaggtcgat gagctcggac tcgatcttcg cgtccaggaa gatcgccttc
    11341 gcgggggcga ccagcgcctc cagctcggcc ttgcgcgcgg catccgtcag caccgactcg
    11401 tcgacgttga agacgaagat cacgggcttc gcggtcagca gacccagctc cttgatcggc
    11461 gcgaggtcga gaccgctcac cgacaacagg atgccgcgct ccagagcgtc ctgagcggct
    11521 ttggccatct ccaggacgac cggctcggtc ttcttgccct tgacctcctt ctcgaggcgc
    11581 acgatggcct tctcgagggt ctgcaggtcg gcgagcatca gctcggcgtt gatggtctcc
    11641 atgtcggatg ccgggttcac cgcgccctcg acatgcacca cgtcgtcgtc ggcgaagccg
    11701 cgcacgacct gcgcgatggc gtcggcctca cggatgttgg cgaggaactg gttgcccagg
    11761 ccctctccct cgctcgcgcc gcgcacgatg ccggcgatgt cgacgaacga caccgcggcg
    11821 ggcaggatgc gctcgctgcc gaagatcccg gcgagcacgt cgaggcgctt gtcgggcagg
    11881 ctgacgaccc ccacgttcgg ctcgatggtc gcgaacgggt agttcgccgc gagcacgtcg
    11941 ttcttggtca gcgcgttgaa gagggtcgac ttgccgacat tcggcagtcc cacgattccg
    12001 atggtaaggg ccacgggcat ccagtctacc ggcggcccgc tactccaacc gcttccagtg
    12061 cccctccgag atcacgtcga cgtcctcccc gacgacgcgg atcgcggtct ggtcgtcgat
    12121 cgcatacgcg ggcccgtcga tgtgctcggc ccaccgctcc gcgtgcgcga gcgtgttctc
    12181 cgggaaatcg gggtagtcca gatgcgggaa cagcgagaag tccacgatgc cgagcgtccc
    12241 ctcccccggc tccggcggcg tccagcgctc gaactccgcg ccgatgcgcg gcgtcatcac
    12301 catgctccct gcgctcacgc cgacccacac ggtccgctcc agacggggga agagcttgaa
    12361 cagccccgaa cgccggatcc agtgcgccag ataggtcgcg tctccgccgt ccacaagcag
    12421 ggcgtcggcc tgctccaccc acgagaccca ccgctccccc tccatcgacg gcagggcggt
    12481 gagttcgagc agaccggcgg acttccagcc gagcgccgtc atcgaccagt cgctgctcgc
    12541 cgagcggagg gtcacctcgg gcgtgcacga cggatgcccg tactgggcgg tcgggatgaa
    12601 cagcgccgtc gaatcctcga tcggcttgcc gagcatctcc accaatgccg tgcggatgct
    12661 ggggttcgtc actccaccgg aggtgagcag cagtctcatc gcgccagcct acgaccgcga
    12721 cgggacaccg ggccgactcg atggggacta gtccccctgc cgtcagacgc cgacgggccg
    12781 ctagcgtggt gccatccgag cagaggagcc catcgtgcca gacgacgtca atgctgacga
    12841 gatcacagac ccgttctggt cgatcgtgcg gcgccgccac cccgacctgg acatcgtgct
    12901 cctcccgccg gcgaagcccg caccggacac atccgggctc gagcctcagg ctccggagac
    12961 gttcgcgcac gagcacctcg ccgaggtcga ccgggcgtgg gccgacctgg tcggtcacgg
    13021 gatgccgctc agcacggccc agtggatccc cgggcccaca cgggactcgg tctgccacag
    13081 tgtcacgctg acgctcggcg aggtcgaaga gtccatcgcg ctggggcatc tgcgcgcggc
    13141 tgccgaactg ctggcacccg gccactggcg ggtcttcctc cctccgaggg ggatgccgcg
    13201 ggtgaccgcc gatcgggacg gccggctcgg cgacgagcac ctgctcttcg gctacgcgat
    13261 cgagaaccgc aacctgttcc tgcgcctcga gtccaccggt gtcccggtcg gcgagcgcac
    13321 cgcgtacgag ctgatcgggg cggcagcatg accgacggcg tggaattcaa tccggcactg
    13381 ctgttcaccg tcatcccgca gtggcgcacg caggcttcgc agatgcgcag cgcggcgagt
    13441 tcactgggag gcgctcccac gggtggcctc ccggcaagcg cggcaggcgc ggcgaagacg
    13501 ttcctgagca tgtgggagaa caccacccgc aagggatccg tcgccgccga cgtgtacgcc
    13561 gatgaactcg agtcgaccgg tacgtcgtac cagaacatcg acgccgagat cgcgcgccgc
    13621 atggatgcga tgggggcggg tgcacgatga cgaccgtcca gatccccgcg ccgctccact
    13681 accccgaccg gccggacggc gatcccgcag ccgcggagac gctggccggc acgctgctgc
    13741 acggttccgc cgtcaccacc gagttcgccg acgtggccgc cctgctctcg gaccccgggt
    13801 acatgtggcg aggtgccgct tcggaggcct acaccgcgca ctcggcgcgc ttcgcgaagc
    13861 agtacgagcc catgggcgag actctcaagc gcgtagcccg cggtgtggac gtcttcgcag
    13921 cagagcttcg cgaactgcag agcgagcaca ccacgctcgt cgggcaggtg acggcctacg
    13981 accgggatcg ggtcgcactg atcgccgatg tcgatgccgg caccgaggcc gatgtgccgg
    14041 cgctgcagga ccgtgcgcgc cgcctgagca cccagcgcac ggggatcgcg gatgacatca
    14101 ccgatttcca gaagcgcacc accgacaacg agcagtatct gactcgcctg ttcaccgggg
    14161 ccgacaccga ggctgaggct caggcacccg gaggcggagt cgacgacatg gcgactctcg
    14221 cgattctgac catgccgctt ttcaaggacc ccaaggccat ggcgaactgg tggaacggcc
    14281 tctcggaggc cgagcagaac gccgtcatcg ccgcctaccc ggagaagatc ggcagcgccg
    14341 acgggcttcc ggcatccgca cgcgacggcg caaaccgtct gctgctggaa tccgacctcg
    14401 ccgaactgca ccagaaggag gccgacggca ccctgaccga ccaggagaag cagcggctca
    14461 agaacgccga ggccacgcag aaggcgctga agtactccga cgactacgtg gtgccgggaa
    14521 cgaacgatca cccgggcgga cagctctggc tgtacgaccc cgacgcgttc aacggcgacg
    14581 ggcgggtggc gatcgccctc ggcgacctcg acaccgccga tgatgtctcg gtctcggttc
    14641 cgggaatcca gacgcagatg gacggcgtgg cggggaacat gaaggacacc gcacacctgt
    14701 acgagagcgc gcggcacagc ggcgacgggt cgagcgtcgc gacgatgttc tggctgggct
    14761 acgacacccc cgccggagac ctgctcgagt ggggcgaggt cccatccgag gatcgcgcga
    14821 cggatggcgg cgcgaggctg gcggatgccg ttgacggcct gcgcgcctcc cgcccggaca
    14881 accccgcgca catgaccgcg atcgggcaca gctacggttc gaccacgacc tcttacgccg
    14941 cgacgaacca ccacctggcc gtcgacgaca tcgcgctcat cggcagcccc ggcaccgggc
    15001 ccgcagatca tgcatccgac ttcagcgtcg gagcggacca cgtctacgac ggacggaaca
    15061 gccgggacct cgtcgcggcg ctcggcgagc agggctggct ccgcaagagc cagctgctcg
    15121 ggctcgggct gggtgtggac ccgtcttcgg aggacttcgg tgctcaccgg ttcgaagccg
    15181 agtccgtcaa ccgcgcggac cacatccgca acatcgacga tcacacccgc tactacgacc
    15241 gtgacagcga gtccctgtac aacctcggcc ggatcgtgga cggccacggt gacgacgtcg
    15301 tgggcgccga ccagtcctac gacccgtggt ggggcccgcc ccaggacccg gagatcgatc
    15361 gcacgcccac cacggacgtg ccgggccgat ccgacacggg cttcggcaac gagccctgat
    15421 gaggaaagca gtcatgatgg gagtcgtggg actcctcgcc ttcctcctcg gcggatgctc
    15481 gtccgtgccg tcggaccctc gagcagtgct cgaggagaag gcggcgacga tcgacgccgc
    15541 cgtgcaggac ctcttcgaga ctctcgccgt ggccggactc caccacgtgt cggcagcgag
    15601 cgccgtcgac acctgtcaga gcgagcccgc gcccggagtc tcgtatcggg cggggatgac
    15661 tgtgaaggcg ggcgaagatc tcgccggcgg cttcgacgcc ctggtgaagc agctcgacgc
    15721 ggccggctgg aagtccacgg atgcctatcg cgacgtcaag atcgatccgg ccaggcccat
    15781 gggccggttc gtccgggacg acatcactct cgatgtgaag accggagggg cctccagcgg
    15841 aggcgtctgg tacggcgccg acgagatgca actcggcgtc acgatcgacg acgactgcgt
    15901 acgggttccc gacggcggct acatcagcaa ggtcgaggac ctcgggaagg acatcctccc
    15961 ccgctcctga gcgcctctcg gtgcgcgcgg gtcagccgac cagtttcagc accgctcgca
    16021 gcgagtcgcc ctcgacatgc cgcgggccgt tcggcggcgc cgcgatccgc tctcggttca
    16081 accagaacgc ggagagcccc gcccccacag cgcccatgac gtcggccggg aagctgtcgc
    16141 cgacatgaag agatgcggcc gagagcactc cggcccgatc gagagcatgc cggaagatct
    16201 cggcgtccgg cttcagcacg ccgatgtccc ccgaggcgac gaccacgtcg aacaggccgt
    16261 cgatcccgac cgtgcgcagc ttctcgcgct ggaacgccga gggcccgttg gtgatcagcc
    16321 cgatcgggat gccccgtcgg cgcagctcca ccagcacgtc acggctgtct tcgaacagcg
    16381 cgaacgtgcg tcgctccgcc tccaggtgca gttccaccgc ctcgtccacg agctccccgc
    16441 ccgcaccggc gcccgacagc gtgcgccgcc acacctcccg cgtcagggcg tcgtcctcca
    16501 gttcgccgcg catccactgc tcgccctgtg atgcccagca tccgctccat accgtcgcgt
    16561 tcgccgtgcg catcgcgtcc gtgtcgagat caggatgccg tgcggcgagc tcgtcgatgg
    16621 cgagcagcgc ggcgtcgacg gcgccgtctc cgaccagagt gtcgtcgagg tcgaagaaga
    16681 cggcgcggat ggattcagtc acggcgtcgc gcccggaacg cggtcgtcac atacggcagg
    16741 tcgatcgtgt cttcgccggt gacgcccagc tcgtcgaaca gggcgtccat gccctcgtgg
    16801 atgctcgcgc gctcctgctc ggcggccgtg atgagatagc tgcgcgaatc cgccatgcgg
    16861 tgcagctgcg cgcgcgtcat ggggcgcacc cactcccagc gctccgcctc cagctcgccg
    16921 aacggcgcgg cgaccgtcgg gccgccggcg tccagcatct cctccgcggc actcgcgtgc
    16981 atgatggcgg tgaaccggcg cacccagtcg acgcggttgt cgcgcgcgtt ccagatcagc
    17041 ccgagcgtcc cgccggggcg cacgacgcgg ccgatctcgg ccgatgccgt ctccgggtcc
    17101 acccagtgcc acgcctgccc gagcacgacg gcatccagac tcgcatccgg aaggggcagc
    17161 gactcggccg tgcccacgta cgtcgggacg ccggggacgg tgtcgcgcag cttggccagc
    17221 atcgccgcat ccggatccac cgccacgacc tgcgcatccg gagcatccgc gatcacccgg
    17281 gtgagcttgc ccgtgccggc tcccacgtct gcgatcctcc gcgcctcgtg cggcagcttc
    17341 tccagcatcc aggcaacggc ctcgaagggg tagtccggcc gcgccgcctc gtaggtgtcg
    17401 gcctcggatc cgaaggaagt cgctcgcacg tcactcatat cgctcagcct acggcgagcc
    17461 tgcgacgtcg gcgccgaggg cggcgcgaga gtaggaccgt gccattggac ttcaccgcga
    17521 tcgacttcga gacggcgaac tccagccccg cctccgcctg ctccgtcgga ctcgtccgcg
    17581 tgcgcgacgg gaaggtcgtc gcgcagaccg gttggctgat ccgcccgcct gcgggacatg
    17641 acgagttcaa cgagtggaac accaggatcc acgggctgta cgcgcacgat gtcgtcaatg
    17701 cggccacctg ggttcagcag atcggcagcc tctgcgcgtt cgccggggcc gacgtgctcg
    17761 tcgcccacaa cgccggcttc gacatgaacg tgctgcgccg cgcctgcgag gcgacgggca
    17821 tcgatgcccc gccgtaccgg tcgctgtgct cgctcgcggt ggcgcgcaag acctacgatc
    17881 tcgactccta ccggcttccc aaggccgctg cggcggccgg cttcggcgac ttcccgcacc
    17941 atgacgccct ggcggatgcg cgggcctgcg cacagatcgt gatcgacgcg gcccggc
//
LOCUS       DATMSX010000561         6746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_132213,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000561 DATMSX010000000
VERSION     DATMSX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132213"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VN041_17825"
     CDS             <1..1239
                     /locus_tag="VN041_17825"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWU30943.1"
                     /translation="IAPAERARLLRSFASVVDAHIDELADLEVAGAGHTIGNARWEAG
                     NVRDVLNYYSAAPERHFGRQIPAPGGVDITFHEPLGVVGIIVPWNFPMPIAGWGFGPA
                     LAAGNTVVLKPAELTPLTAMRLGELALEAGIPEGVFTVIPGKGRIVGDRFVTHPLVRK
                     VCFTGSTDVGKGIMRGCADQVKRLTLELGGKNANIVFEDADLERAAASAPGAGLDNAG
                     QDCCARSRILVQQSVYDRFLELLQPAVESFRVKDPAAEDAEMGPLISAKQRDSVASAL
                     TGADVAFRGQAPDASSGYWLAPTVVMPRGASDPVWSQELFGPVLAVMPFTDEADAIAK
                     ANDTEYGLSGSIFTRDVGRALRVARGVESGNLSVNSHSSVRYWTPFGGFKQSGLGREL
                     GPDAPDSFSEVKNVFISTDN"
     gene            1272..2051
                     /locus_tag="VN041_17830"
     CDS             1272..2051
                     /locus_tag="VN041_17830"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HWU30944.1"
                     /translation="MGKLDGKVGVITGGCSGIGLATARKFVAEGAKVVIGDLDEVNGP
                     RIAEELDGVFVKVNVTDQEQVENLFKVANDTYGHIDIAFNNAGISPASDDSILKTGID
                     AWNLVQDVNLTSVYYCCKAVLPYMLAQGKGSIINTASFVAIMGAATSQISYTASKGGV
                     LAMTRELGVQFARKGIRVNALCPGPVNTPLLQELFAKDPERAARRLIHVPMGRFAEPE
                     EIANAVAFLGSDESSFITAMDFLVDGGLSNAYVTPLESDFE"
     gene            2074..2790
                     /locus_tag="VN041_17835"
     CDS             2074..2790
                     /locus_tag="VN041_17835"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWU30945.1"
                     /translation="MRPVIGISTYRQLANWGDWRHIPADLLPADYALSVERAGGVPVL
                     LPPLGSAESARIALSRIDGLLIAGGADINPARYGQEPHEHVTTWYDDRDAWELWLLAE
                     ADRERLPTLGICRGMQMMAVAAGGSLVQHLPDVVGHGDHGGTPAGYSSTDVTVDGEGR
                     IAALIADRLTVPSHHHQAVDAHPGFRVVARDGDGVVQAMEADGDRFAVGVQWHPETAD
                     DSGVFDGLVAAARERAARRG"
     gene            2843..3733
                     /locus_tag="VN041_17840"
     CDS             2843..3733
                     /locus_tag="VN041_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWU30946.1"
                     /translation="MADKVTKTKSKATKEGDLPIGPTGRPYHGFDVPAPLDSHGPARI
                     IALCNQKGGVGKTTTSINLAASLAEYGRRVLAVDFDPQGALSAGLGITVHDVPTVYDL
                     LLDTKRDPREAIVQSSTPNLDVLPANIDLSAAEVHLVNEVARETILARVLRHVAGEYD
                     VILIDCQPSLGLLTVNALTAAHGVLIPLECEFFALRGVALLIETIDKVRDRLNPSIKL
                     DGLLATMYDPRTLHSREVLERVVEAFGDDVLETVIGRTVKFPDASVSGVPITEFAPEH
                     AAAQAYLRLARELVARGAVA"
     gene            3717..4553
                     /locus_tag="VN041_17845"
     CDS             3717..4553
                     /locus_tag="VN041_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWU30947.1"
                     /translation="MAPSPEDTSTGSAAQEADAPGFRVSLSNFDGPFDLLLNLISKHE
                     LDITEVSLSKVTNEFISYLRELDGPDELDQASEFLVVAATLLDMKVAGLLPQGELVDA
                     ESVALLEARDLLFARLLQYRAFKEVSAWFARWLQREDRRHARAAPLDEKHRKKTPELV
                     WSLSVDDFAALALLAFAPKEIPSVGLEHLHAPLVSIREQAAVVVTLLRSTDSLTFREL
                     VSGVSEPGIVVARFISVLELYRHAALSFEQLEPLGELTLRWSAENWSDEQLATLGADY
                     DR"
     gene            4582..5172
                     /gene="scpB"
                     /locus_tag="VN041_17850"
     CDS             4582..5172
                     /gene="scpB"
                     /locus_tag="VN041_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWU30948.1"
                     /translation="MSEDAVLETTLETPLSERVEAILLVVDEPIALVALAAAVHAPVP
                     AVRQTLETLVDDYDGRGTGPRRGFELREVGGGWRLYVREDHDDLVADFIGGQAPARLS
                     QAALETLAVIAYKQPVTRGQVASIRAVNVDSVVRTLLARGLITELFADSETGAINYGT
                     TDQLLQHLGINSIDELPPISPLLDDGADGFDEGVTR"
     gene            5169..5963
                     /locus_tag="VN041_17855"
     CDS             5169..5963
                     /locus_tag="VN041_17855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWU30949.1"
                     /translation="MTTAGNQDAEGVRLQKALANAGVASRRVIEQYIVEGRIRVNGRT
                     VSELGTRIDPETDLIDVDGTAVQLDVSKRYVMLNKPTGIYSTMRDEKGRPDLRRFTKE
                     WEERLYNVGRLDAETSGLLILTNDGELAHVLAHPSFGVTKVYIAKVAGTVTAQTIAKL
                     TRGFDLEDGPIKADKARLLDASRGSSLVELTLHSGRNRIVRRMMAAVGHPVEELVRRQ
                     FGPLHLGTLPVGKARELTKIERGALLTLSRQDTPAAAGDDDKQETE"
     gene            5960..>6746
                     /locus_tag="VN041_17860"
     CDS             5960..>6746
                     /locus_tag="VN041_17860"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HWU30950.1"
                     /translation="MSDTTNARASRVSGTVRIVGAGLLGASIGHALRSHDVDVVLSDA
                     SPSQLRLAIDYGAGRAARDDDAPNLIVVAVPPDVTADVIQAELEAHPDAVVTDVASVK
                     LEPYQELRRRGVDLTRYIGSHPLAGRERGGAISARADLFIGRPWVVCRDEETRAADLA
                     LVEALALDLGATPLEMTPEEHDRSVALTSHVPQVVASLLAARLADADADALQLAGQGV
                     RDTTRIAASAPELWVQILSANAEPVVEVLDALASDLRAVSDALR"
BASE COUNT         1068 a         2310 c         2365 g         1003 t
ORIGIN      
        1 atcgcgccgg cggagcgggc ccggctgctg cgctcgttcg cctcggtcgt cgacgcgcac
       61 atcgacgagc tcgccgacct cgaggtcgcg ggagcggggc acaccatcgg caacgcccgc
      121 tgggaggccg gcaacgtccg cgacgtgctg aactactaca gcgctgcgcc cgagcgtcac
      181 ttcggcaggc agatcccggc accgggcggt gtggacatca cgttccacga gccgctcggc
      241 gtggtcggca tcatcgtgcc gtggaacttc cccatgccga tcgccggctg gggcttcggc
      301 ccggctctcg cggcgggcaa caccgtcgtg ctcaagcccg ccgagctcac cccgctcacc
      361 gccatgcggc tcggcgagct ggcactcgaa gcaggcatcc cggagggcgt gttcaccgtc
      421 atcccgggca agggccggat cgtcggcgac cgtttcgtga cgcatccgct ggtgcgcaag
      481 gtgtgcttca ccgggtccac cgacgtcggc aagggcatca tgcgcggctg cgccgaccag
      541 gtcaagcgcc tgacgctgga gctcggcggc aagaacgcga acatcgtctt cgaggacgct
      601 gacctcgaac gggccgcggc atccgcgccc ggagcaggcc tcgacaacgc ggggcaggac
      661 tgctgcgccc gctcccgcat cctcgtgcag cagagcgtgt acgaccggtt cctggagctg
      721 ctgcagcccg ccgtggagtc gttccgcgtg aaggaccccg ctgccgagga cgcggagatg
      781 gggccgctca tctcggcgaa gcagcgcgat tcggtggcat ccgctctgac gggcgccgac
      841 gtggcgttcc gcggtcaggc cccggacgcc tccagcgggt actggctcgc cccgaccgtg
      901 gtcatgccgc ggggagcgtc cgacccggtg tggtcgcagg agctgttcgg gccggtgctg
      961 gccgtcatgc cgttcaccga cgaagcggat gccatcgcca aggccaatga caccgagtac
     1021 ggcctgtccg gctcgatctt cacccgcgac gtcggccgtg cgctgcgcgt cgcgcgcggc
     1081 gtggagagcg gcaacctgtc ggtgaactcg cactcctcgg tgcggtactg gaccccgttc
     1141 ggcgggttca agcagtcggg tctggggcgc gagctcggac cggacgcacc ggactccttc
     1201 tccgaggtga agaacgtctt catctcgacc gacaattgat cagatctcag gaaaggcaac
     1261 agggagaaca gatgggaaag ctggacggca aggtcggcgt catcaccggc ggatgcagcg
     1321 ggatcggcct cgcgacggcg aggaagttcg tcgcggaagg tgccaaggtc gtgatcggcg
     1381 acctcgatga ggtgaacggt ccgcgcatcg ccgaggagct cgacggcgtg ttcgtgaagg
     1441 tgaacgtcac agaccaggag caggtcgaga atctgttcaa ggtcgcgaac gacacctacg
     1501 gtcacatcga catcgccttc aacaacgccg gcatctcccc ggcatccgac gactcgatcc
     1561 tgaagaccgg tatcgatgcc tggaacctgg tgcaggacgt caatctcacg agcgtctact
     1621 actgctgcaa ggccgtgctc ccgtacatgc tcgcgcaggg caagggatcc atcatcaaca
     1681 ccgcctcgtt cgtcgcgatc atgggagccg ccacctcgca gatctcgtac acggcgtcca
     1741 agggcggcgt gctggcgatg acccgtgagc tgggcgtgca gttcgcgcgc aagggcatcc
     1801 gcgtgaacgc gctctgcccc ggcccggtga acacgccgct gctgcaggag ctgttcgcga
     1861 aggaccccga gcgggccgcg cgccgactga tccacgtgcc catgggacgc ttcgccgagc
     1921 cggaggagat cgcgaacgcg gtagccttcc tgggcagcga cgaatccagc ttcatcaccg
     1981 cgatggactt ccttgtggac ggaggtctgt ccaacgccta cgtcacaccc ctggagagcg
     2041 actttgagtg atccttcgcc ggagcaggcg gccgtgcggc ccgtcatcgg catcagcacc
     2101 tatcggcagc tggccaattg gggcgactgg cgccacatcc cggctgacct gctgcccgcc
     2161 gactacgcgc tctcggtgga gcgcgccggt ggtgtgccgg tgctgctccc gccgctcggc
     2221 tcggccgagt ccgcgcggat cgcgctgagc cggatcgacg gtctgctgat cgcgggcggg
     2281 gcggacatca accccgcccg ctacgggcag gagccccacg agcacgtcac cacctggtac
     2341 gacgacaggg atgcctggga gctgtggctg ctcgccgagg cggatcggga gcggctgccg
     2401 acgctgggca tctgccgcgg gatgcagatg atggcggtgg ccgccggcgg gtcgctggtg
     2461 cagcacctgc cggatgtcgt cgggcacggc gatcacgggg gcaccccggc cggatacagc
     2521 tcgaccgatg tcacggtcga cggggaaggg cgcatagccg cgctgatcgc cgatcgtctg
     2581 acggtgccga gccatcatca tcaggccgtc gacgctcacc ccggcttccg tgtggtcgcc
     2641 cgcgacggcg acggggtcgt gcaggccatg gaggccgacg gcgaccgctt cgcggtgggg
     2701 gtgcagtggc acccggagac cgccgacgat tcgggcgtgt tcgacgggct cgtggcggcg
     2761 gccagggagc gcgccgcgcg ccgaggctga gccggagggc cctgccgcta gactcgagca
     2821 agcacgacga gcaggagaac cggtggcgga caaggtgacg aagaccaagt cgaaggcgac
     2881 gaaggagggc gacctcccga tcggcccgac gggtcgcccg taccacggct tcgacgtccc
     2941 tgcgccgctg gactcgcacg gccccgcgcg catcatcgcc ctgtgcaacc agaagggcgg
     3001 cgtcggcaag acgaccacgt cgatcaacct ggccgcttca ctcgcggagt acggtcgccg
     3061 ggtgctggcc gtcgacttcg acccgcaggg cgcgctgtcg gcaggcctcg gcatcaccgt
     3121 gcacgatgtg ccgaccgtct acgacctgct gctggacacc aagcgcgacc cgcgcgaggc
     3181 gatcgtgcag tcctcgaccc ccaacctcga cgtgctgccg gcgaacatcg acctgtccgc
     3241 cgccgaggtg cacctggtca acgaggtcgc ccgagagacg atcctcgccc gcgtgctgcg
     3301 gcacgtcgcg ggggagtacg acgtcatcct gatcgactgc cagccctcgc tcggactgct
     3361 caccgtcaat gccctgaccg ccgcgcatgg cgtgctcatc cctctggagt gcgagttctt
     3421 cgcactgcgc ggtgtggccc tgctgatcga gaccatcgac aaggtgcgcg accggctgaa
     3481 cccgtcgatc aagctcgacg gactgctcgc caccatgtac gacccgcgca cgctgcactc
     3541 ccgcgaggtg ctggagcgcg tcgtcgaggc cttcggcgac gacgtgctgg agaccgtgat
     3601 cggccgcacc gtgaagttcc cggacgcctc ggtgtcgggc gtgcccatca ccgagttcgc
     3661 gccggagcac gcagccgccc aggcctacct gcgactggcg cgggagctgg tcgcccgtgg
     3721 cgccgtcgcc tgaggacact tcgacgggct cggcggccca ggaggcggat gcgcccgggt
     3781 tccgggtctc gctgtcgaac ttcgacggcc cgttcgacct gctgcttaac ctgatctcca
     3841 agcacgagct ggacatcacc gaggtgtcgt tgagcaaggt gaccaacgag ttcatctcgt
     3901 atctgcgcga gctcgacggg cccgacgaac tggatcaggc atccgagttc ctcgtcgtcg
     3961 ccgcgaccct gctcgacatg aaggtcgcgg ggctgctgcc gcagggcgaa ctggtggatg
     4021 ccgagtccgt cgcgctgctc gaggcccggg acctgctgtt cgcccggctc cttcagtacc
     4081 gcgccttcaa agaggtctcc gcctggttcg cgcgctggct gcagcgggag gaccgccggc
     4141 acgcacgggc ggctccgctc gacgagaagc accggaagaa gactccggag ctggtgtggt
     4201 cgctgagcgt cgacgacttc gcggcgctcg ccctgctggc gttcgcgccg aaggagatcc
     4261 cctcggtcgg gctcgagcac ctgcacgcgc cgctggtcag catccgggag caggccgcgg
     4321 tcgtcgtcac gctgctgcgc agcaccgact cgctgacgtt ccgtgaactg gtgagcgggg
     4381 tgagcgagcc cggtatcgtc gtggcgcgct tcatctcggt gctcgaactg taccggcacg
     4441 ccgcgctctc gttcgagcag ctggagcctc tgggcgagct gaccctgcgc tggtcggcgg
     4501 agaactggtc ggatgagcag ctggcgacgc tcggcgcgga ctacgacagg tgagcggatc
     4561 atgacagatg aggaattccg gatgagtgag gatgcggtgc tcgagacgac cctcgagacc
     4621 ccgttgagcg agcgcgtcga ggcgatcctg ctggtcgtcg acgagccgat cgcgctggtc
     4681 gccctcgcgg cggccgtgca cgcgcccgtc cccgccgtgc ggcagacgct cgagacgctg
     4741 gtcgacgact acgacgggcg cggcaccgga ccgcggcgcg gattcgagct gcgcgaggtg
     4801 ggcggcggct ggcggctcta cgtccgcgag gaccatgatg acctggtcgc ggacttcatc
     4861 ggcggacagg cgcccgcacg cctgtcgcaa gccgcgctgg agaccctcgc cgtcatcgcg
     4921 tacaagcagc cggtcacgcg agggcaggtg gcatccatcc gcgccgtgaa cgtggactcg
     4981 gtcgtgcgca cgctgctggc gcgcggactg atcaccgagc tgttcgccga ctccgagaca
     5041 ggcgcgatca actacggcac caccgaccag ctgctgcagc atctggggat caactcgatc
     5101 gacgagctgc ccccgatctc gcccctgctc gatgacggtg cagacggctt cgacgaggga
     5161 gtgacccgat gaccaccgca ggaaaccagg acgccgaggg cgtgcggctg cagaaggcgc
     5221 tggcgaacgc gggtgtcgca tcgcgacgcg tgatcgagca gtacatcgtc gagggtcgca
     5281 ttcgagtcaa cggccgcacg gtcagcgagc tcggcacgcg cattgacccg gagaccgatc
     5341 tgatcgacgt ggacggcacg gccgtgcagc tggacgtgtc caagcggtac gtcatgctca
     5401 acaagcccac cggcatctac agcaccatgc gcgacgagaa gggccgcccc gacctgcgcc
     5461 gcttcacgaa ggagtgggag gagcgcctct acaacgtcgg ccggctggat gccgagacca
     5521 gcgggctgct gatcctcacc aacgacggcg aactcgcgca cgtgctcgcg cacccctcat
     5581 tcggggtgac gaaggtgtac atcgcgaagg tcgccggcac ggtgaccgcg cagaccatcg
     5641 ccaagctgac ccgcggcttc gacctggagg acggccccat caaggccgac aaggcgaggc
     5701 tgctggacgc gtcgcgcggg agcagcttgg tcgagctgac cctgcactcc gggcggaacc
     5761 ggatcgtgcg tcgcatgatg gccgccgtcg gtcatccggt cgaggagctc gtgcgtcgcc
     5821 agttcggtcc gctccacctg ggaactctcc cggtcgggaa ggccagggag ctgactaaaa
     5881 tcgaacgagg cgcattgctg actctgtcgc gccaggacac acctgcggcc gcgggagatg
     5941 acgacaagca ggagaccgag tgagcgacac cacgaacgcc cgcgcatcgc gcgtgtcggg
     6001 caccgtgcgc atcgtcggcg cgggcctgct cggcgcgagc atcggacacg cgctgcgctc
     6061 gcacgacgtg gatgtcgtgc tctccgacgc ttcgccctcg cagttgcggc tcgcgatcga
     6121 ctacggggca gggcgcgcgg cgcgcgacga cgacgctccg aacctgatcg tggtggccgt
     6181 gccgccggac gtgaccgccg acgtcatcca ggcagagctc gaggcgcacc cggatgccgt
     6241 ggtgaccgac gtcgccagcg tgaagctgga gccgtaccag gagctgcgcc gtcggggcgt
     6301 cgacctcacc cgttacatcg gctcgcatcc gctcgcggga cgtgagcgcg gcggtgcgat
     6361 ctcggcgcgc gccgacctgt tcatcggtcg tccgtgggtg gtgtgccgcg acgaggagac
     6421 ccgcgccgcg gacctcgcac tggtcgaggc gctcgccctg gacctcggcg ccacgccgct
     6481 ggagatgacg ccggaggagc acgatcgctc ggtcgcgctg acctcgcacg tgccccaggt
     6541 ggtggcgagc ctgctggccg cccgcctggc ggatgccgat gcagacgcgc tgcagctggc
     6601 gggccagggt gtgcgcgaca cgactcgcat cgcggcatcc gcccctgagc tgtgggtgca
     6661 gatcctgagc gcgaacgccg agcccgtcgt cgaggtgctc gacgccctcg catccgacct
     6721 gcgtgccgtc tccgacgcgc tgcgcg
//
LOCUS       DATMSX010000562         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_103466,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000562 DATMSX010000000
VERSION     DATMSX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103466"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VN041_17865"
     CDS             complement(<1..989)
                     /locus_tag="VN041_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU30951.1"
                     /translation="MVSIDEVAKLAGVSTATVSRALSGRGHVSAGARDRVHVAAETLG
                     YVVSSRASSLASGRTQNVGVIVPFLDRWFFSTVLSGATSALMRAGYDITLYNITADAA
                     VRREVFSTFLRRQRVDAVVAVSIELDDDETGQLLDLGLPVIAIGGPNPRLRTLTVDDT
                     AVARLATDHLIGLGHRDIAHIGANPEFDIDFHIPTQRRLGFEQALTDAGITPNHAFLE
                     PADFTVDGGFRAAKQLLGRPGPRPTAIFAASDEMAIGAILAARDLGFRVPQDLSIVGI
                     DGHELGEFFQLTTVDQFPMAQGERAAQAILAQLEGGADAPGGELPYELIVRGTT"
     gene            complement(1096..2757)
                     /locus_tag="VN041_17870"
     CDS             complement(1096..2757)
                     /locus_tag="VN041_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HWU30952.1"
                     /translation="MAQLEQVASPGSEWWRTAVIYQIYPRSFADASGDGIGDLPGITA
                     RLDDLKSLGVDAIWLSPFMTSPQKDAGYDVADYRDVDPLFGTLADFDAMLGAAHARGI
                     RVIVDLVPNHSSDQHVWFQQALKAAPGSPERARYVFRDGRGENGELPPNNWESVFGGG
                     MWERVTEADGTPGQWYLHIFDASQPDFDWTNEEVREEFRSILRFWLDRGVDGFRVDVA
                     HGMVKEAGLPDYAPVADADSMGGGEENVPYWGQDEVHDIYRDWHKVMAEYDGDRALCG
                     EAWLPTLKKTALWVRPDEMHQTFNFPYLMTEWDAAALRAVIRDSLDAFGEVGAPSTWV
                     LSNHDVIRHASRLALTADSPQGDGIGPKSKGKPDPVVGLSRGRAATTVMLSLPGSAYV
                     YQGEELGLPEAMEIPDEFRQDPTWFRTNGERYGRDGCRVPLPWTASGTAFGFNDTGAS
                     WLPQPAEWAGFARDAEEQDPTSTLNLYKALLAERRARALGSGSLVWDDVDPAAVAFHR
                     GDLHVVANLGAEPIELGEGVTFVIQSEPFAGTALPPNTAAWFTRA"
BASE COUNT          435 a         1034 c         1114 g          459 t
ORIGIN      
        1 gtcgtgccgc ggacgatcag ctcgtagggc agctcgccgc cgggggcatc cgccccgccc
       61 tcgagctgcg cgaggatcgc ctgcgcggcg cgctcgccct gcgccatcgg gaactggtcg
      121 acggtcgtga gctggaagaa ctcgcccagc tcgtgcccgt cgatcccgac gatcgagagg
      181 tcctgtggca cccggaagcc cagatcgcgg gcggcgagga tcgcgccgat cgccatctcg
      241 tcggatgccg cgaagatcgc cgtcgggcgc gggcccgggc gccccagcag ctgcttggcg
      301 gcccggaagc cgccgtcgac ggtgaagtcc gcaggctcca ggaaggcgtg gttcggtgtg
      361 atcccggcat ccgtcagcgc ctgctcgaag ccgagccggc gctgggtcgg gatgtggaag
      421 tcgatgtcga actccgggtt cgcgccgatg tgcgcgatgt cgcggtgtcc gaggccgatc
      481 aggtgatcgg tcgccaggcg ggcgaccgcg gtgtcgtcga cggtgagcgt gcgcagtcgc
      541 ggattcggcc cgccgatcgc gatcaccggc agccccaggt cgagcagctg cccggtctcg
      601 tcgtcgtcga gctcgatgga caccgcgacg accgcgtcca cgcgctggcg acgcaggaac
      661 gtggagaaca cctcgcgccg gacggcggca tcggccgtga tgttgtagag cgtgatgtcg
      721 tagcctgcgc gcatcagcgc gctggtcgcg ccggagagca ccgtgctgaa gaaccagcgg
      781 tccaggaacg ggacgatcac gccgacgttc tgcgtgcggc cggatgccag gctcgacgcc
      841 cgcgaggaga cgacgtaccc gagggtctcg gctgcgacgt gcacgcgatc gcgggcgccg
      901 gccgagacgt gtccccgccc gctgagcgct cgcgacacgg tcgcggtcga cacccccgcg
      961 agcttcgcga cctcgtcgat gctcaccatg tcgtcactcc tgctcttctg gtgccgcggg
     1021 accggcctcg cggcaggagg cggccaccgg gaggggtggc cgtctcacgc cgcaaggcaa
     1081 gtctcgcgca cgggatcagg cccgggtgaa ccaggctgcg gtgttcggag gcagtgcggt
     1141 gccggcgaac ggctcggact ggatcacgaa cgtgacgccc tcgccgagct cgatcggctc
     1201 ggcgcccagg ttcgccacca cgtgcaggtc gccgcggtgg aacgccacag cagcgggatc
     1261 cacgtcgtcc cacaccagcg agccggagcc gagcgcgcgc gcacggcgct ccgcgagcag
     1321 cgccttgtac aggttcaggg tcgaggtcgg gtcctgctcc tcggcatccc gcgcgaagcc
     1381 cgcccactcg gccggctgcg gcagccagga tgcgccggtg tcgttgaatc cgaacgccgt
     1441 gccggatgcc gtccacggca gcggcacgcg gcagccgtcg cggccgtagc gctcaccgtt
     1501 cgtgcggaac caggtggggt cctggcggaa ctcgtccggg atctccatcg cctcggggag
     1561 gcccagctcc tcgccctggt agacgtacgc cgaaccgggg agggacagca tgacggtggt
     1621 cgccgcacgc ccgcgggaga gtccgacgac cgggtcgggc ttgcccttcg acttcgggcc
     1681 gatgccgtcg ccctgcgggc tgtccgcggt cagggccagg cgcgaggcgt gccggatcac
     1741 atcgtggttc gacagcaccc aggtgctggg ggcgcccacc tcgccgaagg cgtcgaggga
     1801 gtcccggatg acggcgcgca gtgccgcggc atcccactcc gtcatcaggt acgggaagtt
     1861 gaaggtctgg tgcatctcgt cggggcgcac ccagagcgcg gtcttcttca gggtgggcag
     1921 ccaggcctcc ccgcacagtg cgcggtcgcc gtcgtactcg gccatcacct tgtgccagtc
     1981 gcggtagatg tcgtgcacct cgtcctggcc ccagtacggc acgttctcct cgccgccgcc
     2041 catggagtcc gcgtcggcca cgggcgcgta gtccggaagg ccggcctcct tgaccatccc
     2101 gtgggcgacg tccacgcgga agccgtcgac gccgcggtcg agccagaagc gcaggatgct
     2161 gcggaactcc tcgcgcacct cctcgttggt ccagtcgaag tcgggctggc tggcatcgaa
     2221 gatgtgcagg taccactgtc cgggcgtgcc gtcggcctcg gtcacgcgct cccacatgcc
     2281 gccgccgaac acgctctccc agttgttcgg gggcagttcg ccgttctcgc ctcgtccgtc
     2341 gcggaacacg tagcgggcgc gctcggggct gccgggcgcg gccttgagag cctgctggaa
     2401 ccatacgtgc tggtcgctgg agtggttggg gacgaggtcg acgatcacgc ggatgccgcg
     2461 ggcgtgcgcc gcaccgagca tggcgtcgaa gtcggcgagc gtgccgaaca gcgggtcgac
     2521 gtcgcggtag tcggccacgt cgtagccggc gtccttctgc gggctcgtca tgaacgggct
     2581 cagccagatc gcgtcgacgc cgaggctctt caggtcgtcg agtcgcgcgg tgatgcccgg
     2641 caggtcgccg atgccgtcgc ccgaggcgtc ggcgaacgag cgcggataga tctggtagat
     2701 gacggccgtg cgccaccatt cggaaccggg ggatgcgacc tgctcgagct gtgccatgcg
     2761 aacaggctac tggaagcgct tacagttccg gaagtgcagg gatcgaatcg gttcgattcg
     2821 ggcgtggcgg ccggggagag gtgtaagtcg agtgcgcgcc acggtcactt ctgaaggatg
     2881 aacgcggttc tgaaggacgc tcggcgccgg tgcatccttc agaaccgcga gtgtccttca
     2941 gaagagcgga tgctgcgccc gccggcgact gcggatgcag cgctctgccg tcatgggcgc
     3001 ccgcgcaggg catccgtccg cggctcaggc cgaacgtcgc gc
//
LOCUS       DATMSX010000563         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_17801,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000563 DATMSX010000000
VERSION     DATMSX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17801"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VN041_17875"
     CDS             complement(<1..118)
                     /locus_tag="VN041_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU30953.1"
                     /translation="MNLQQLQILRELGALGSVTAVAEALRVSPSAVSQQLAAL"
     gene            206..1249
                     /gene="tdh"
                     /locus_tag="VN041_17880"
     CDS             206..1249
                     /gene="tdh"
                     /locus_tag="VN041_17880"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWU30954.1"
                     /translation="MKALFKPAAAAGLELVDRPDPTAGADEVVIRVLRTGICGTDLHI
                     LRWDDWAASAVNAPLTPGHEFYGEVVEIGPLVHDIKVGDRVSGEGHIVCGTCRNCRAG
                     RRHMCIRTIGLGVQRDGAFAEYVSLPATNVWVHHDDVTPELGAIFDPLGNAVHTALTY
                     PLVGEDVLVTGCGPIGLMSIAVARHAGARFITATDVSGPRLEMALTMGADQAIDVSQH
                     DIREAQTALGMREGFDIGFEMSGAPSALPAMIDNMNHGGRIAMLGLPSAGFDIDWGKL
                     VTHMLTIKGIYGREMFETWNAMGAMLQTSAVLRDSISRVIAEVIPARDWEKGFAAAAS
                     AGTGKIILDWTEL"
     gene            1259..2437
                     /locus_tag="VN041_17885"
     CDS             1259..2437
                     /locus_tag="VN041_17885"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWU30955.1"
                     /translation="MYTTFKDHVAAELAGIEEAGLTKHERGIRGPQSAEITADGTEVL
                     NFCANNYLGLADDPRIVEAAKSALDEWGYGMASVRFICGTQEQHLELERRLSAFLGTD
                     DAILYSSCFDANGGVFETLFGAEDAIISDELNHASIIDGIRLSKARRFRYRNSDMADL
                     EQQLKDAADARFRVIVTDGVFSMDGYIAKLPEICDLAEKYDALVFVDDSHAVGFIGEN
                     GRGTPELCGVSDRVDIYTGTFGKALGGASGGYVASHRDIVALLRQRSRPYLFSNTLAP
                     AIVAGTLTALDLVEGSADLRARLRENAELFRRRMTEEGFDLQPGEHPIVPVMFGDAAL
                     TARIAKAMQAHGVYVTAFSFPVVPRGLARIRVQLSAAHTPEQVERCVAAFVAARAAVD
                     "
     gene            2478..3659
                     /locus_tag="VN041_17890"
     CDS             2478..3659
                     /locus_tag="VN041_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30956.1"
                     /translation="MDAELTARRGLIDQLTEQGSIRSARVRDAFERVRRTAFIPDTAL
                     EEVYADRTHTIAEDDGVKVSTVSAPSMQALMLEIAAPLPGEDVLEIGSGGTNAALLAE
                     MVAPGGSVWTVDIDDRVVERARASLGRHPAPVPVRVDRIDAWEAGTPIAAGSFDLVEF
                     TVDTWTFPSWLPEVLPTGGRVVAPMSICGVGCGWALEKDDDRLIGRTSFGGGFVGARG
                     AGAEGDEPEEIRSDDVVLAPDRDLETGWLASVLSREGEATGVVTHRAIGSLYLPLFLR
                     LLCGSERAGSIRSASRSKANGFPGASWMRTPVWLADDGLAVIDHRVAGDGVDIFVRSF
                     GSDGPFLAERMAERLRTWQEGEGGPVFEAIPVDAVRKAAPEGVRVLRRGSWEFRLSWL
                     A"
     gene            3786..4394
                     /locus_tag="VN041_17895"
     CDS             3786..4394
                     /locus_tag="VN041_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU30957.1"
                     /translation="MTEHEEVRTLDAGALKALAHPLRVRIYDLLSERGPQTASSLAAL
                     IGETSGATSYHLRALAAKDLIREVEGRGTARERWWERPKGRINLPGPDDRMSPANRAA
                     AQIVTSEFFRLRHQTLMDYLNRPDSEQPEEWRDAGLVMTTTLDLTPEQMSELKQELSD
                     AVDAAIERYRGQEGAPGTRRVSLRAEIFDLPAAISRGTTDQE"
     gene            4397..4618
                     /locus_tag="VN041_17900"
     CDS             4397..4618
                     /locus_tag="VN041_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30958.1"
                     /translation="MTAATLHLASPTTTERALLKIADAITAYTENRRLLRAERREIAL
                     DLLREQQARKQDPRALEYAMLSIGSRPRR"
     gene            complement(4688..4903)
                     /locus_tag="VN041_17905"
     CDS             complement(4688..4903)
                     /locus_tag="VN041_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30959.1"
                     /translation="MQLGTRWATGSEPPASVPADLRPAIAEVEERGLTGHWTLTWLEG
                     RPLAELDAGWEVLLTASGDVIARPFED"
     gene            complement(4906..>5109)
                     /locus_tag="VN041_17910"
     CDS             complement(4906..>5109)
                     /locus_tag="VN041_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30960.1"
                     /translation="IRRAKTEAKASQKTPVSTATIAAPAAKIEALRAAADDLRAVGRI
                     AELSFAEAEELAVTAIELAPVEA"
BASE COUNT          819 a         1748 c         1776 g          766 t
ORIGIN      
        1 gcagcgccgc gagctgctgt gagacggcgg acggcgagac gcgcagcgcc tcggccacgg
       61 ccgtgacgct gccgagcgcg ccgagctcac gcaggatctg gagctgctgc aggttcatgg
      121 cctcagtcaa tcatgaagtg gatcttcatc gattatccag agagtgccga ttgatcttca
      181 agttcggatg cggtctcatc gaagtatgaa ggcccttttc aagccggcag ccgccgccgg
      241 actcgagctc gtcgatcgcc cggaccccac cgcaggagcc gacgaggtcg tcatccgggt
      301 gctgcgcacc ggcatctgcg gcacggatct gcacatcctc cgctgggacg actgggcggc
      361 ctccgccgtc aacgccccgc tcacccccgg ccacgagttc tatggcgagg tcgtggagat
      421 cggcccgctg gtacacgaca tcaaggtcgg cgaccgggtg tccggcgagg gtcacatcgt
      481 ctgcggcacc tgccgcaact gccgtgccgg ccgccggcac atgtgcatcc gcacgatcgg
      541 cctgggcgtg cagcgcgacg gcgcgttcgc cgagtacgtg tcgctgcccg cgaccaacgt
      601 ctgggtgcat cacgacgacg tgacgcccga gctcggggcg atcttcgacc cgctcggcaa
      661 cgccgtgcac accgcgctga cgtacccgct cgtcggcgag gacgtgctcg tcaccggctg
      721 cggtccgatc ggcctgatgt cgatcgccgt cgcccgccac gccggagcgc gcttcatcac
      781 ggcgaccgac gtcagcggcc cgcgtctgga gatggcgctg accatgggcg ccgatcaggc
      841 catcgacgtc tcccagcacg acatccgcga agcgcagacc gccctcggca tgcgcgaggg
      901 cttcgacatc ggcttcgaga tgagcggtgc cccgagtgcg ctgcccgcca tgatcgacaa
      961 catgaaccac ggcggccgga tcgccatgct cggcctgccc agcgccggat tcgacatcga
     1021 ctggggcaag ctggtcacgc acatgctcac catcaagggc atctacggcc gcgagatgtt
     1081 cgagacctgg aacgccatgg gggccatgct gcagaccagt gccgtgctgc gcgactcgat
     1141 cagccgcgtc atcgccgagg tcattccggc gcgcgactgg gagaagggct tcgctgccgc
     1201 ggcatccgcc ggcaccggca agatcatcct cgactggacc gagctgtaag gaaccgagat
     1261 gtacacgact ttcaaggacc acgtggccgc agagctggcc ggcatcgaag aggcggggct
     1321 caccaagcac gagcgcggca tccgcggccc ccagagtgcc gagatcaccg ccgacggcac
     1381 cgaggtgctg aacttctgcg ccaacaacta cctcggcctc gccgacgacc cccgcatcgt
     1441 ggaggcagcc aagagcgccc tcgacgagtg gggctacggc atggccagcg tgcgcttcat
     1501 ctgcggcacg caggagcagc acctcgagct cgagcgacgc ctgtcggcgt tcctcggcac
     1561 cgacgacgcc atcctgtact cctcgtgctt cgacgcgaac ggcggcgtct tcgagacgct
     1621 gttcggcgcg gaggacgcca tcatctcgga cgagctgaac cacgcctcga tcatcgacgg
     1681 catccgcctg tccaaagccc gccgcttccg ttaccggaac agcgacatgg cggatctcga
     1741 gcagcagctg aaggatgccg cggatgcccg cttccgcgtg atcgtgaccg acggcgtgtt
     1801 ctcgatggac ggctacatcg ccaagctgcc ggagatctgc gacctcgcgg agaagtacga
     1861 cgccctggtg ttcgtcgacg actcgcacgc ggtgggcttc atcggtgaga acggccgtgg
     1921 cactcccgag ctgtgcgggg tgtcggaccg cgtcgacatc tacacaggca ccttcggcaa
     1981 ggcgctgggc ggggcatccg gcggctacgt cgcctcccac cgcgacatcg tggcgctgct
     2041 gcggcagcgc tcgcgtccgt acctgttctc gaacaccctg gccccggcga tcgtcgcggg
     2101 cacgctcacc gcgctggacc tggtggaggg ctccgccgat ctccgcgcgc gcctgcgcga
     2161 gaacgccgag ctgttccgga ggcggatgac ggaggagggc ttcgacctgc agccggggga
     2221 gcaccccatc gtgcccgtga tgttcggcga cgccgcgctg accgcgcgca tcgcgaaggc
     2281 gatgcaggcg cacggcgtgt acgtcacggc gttcagcttc ccggtggtgc cgcgcgggct
     2341 cgcccgcatt cgcgtgcagc tctccgcggc gcacacgccg gagcaggtgg agcgctgcgt
     2401 agccgccttc gtcgcggcgc gcgcagcagt ggactgagcc cgagcgggat gtcggtgacc
     2461 ccgtgcagga tcggctcatg gatgccgaac tcaccgcgcg acgaggactg atcgaccagc
     2521 tgaccgagca ggggagcatc cgctccgcgc gtgtgcggga tgcgttcgag cgggtgaggc
     2581 gcacggcgtt catccccgac acggcgctgg aggaggtcta cgcggatcgc acccacacca
     2641 tcgcggagga cgacggcgtc aaggtcagca cggtctcggc tccctcgatg caggcgttga
     2701 tgctcgagat cgccgcgcct ctgccgggcg aagacgtcct cgagatcggc tccgggggta
     2761 cgaacgccgc gctgctggcc gagatggtcg caccaggcgg atccgtgtgg acggtcgaca
     2821 tcgacgaccg ggtggtcgaa cgcgcacggg cgtcgctggg tcggcatccc gcgcccgtgc
     2881 cggtgcgggt cgaccgcatc gacgcctggg aggcgggtac gccgatcgcc gctgggtcat
     2941 tcgacctggt cgagttcacc gtcgacacct ggaccttccc ctcctggctg ccggaggtgc
     3001 tgccgacagg cggccgagtg gtcgcaccga tgagcatctg cggtgtcgga tgcggctggg
     3061 cgctcgagaa ggatgatgac cggctgatcg gaaggacctc gttcggcggg ggattcgtcg
     3121 gcgcgcgcgg tgcgggtgcg gagggcgacg agccggagga gatccggagc gacgacgtcg
     3181 tgctcgctcc cgatcgcgac ctggagacag gatggctggc ctcggtgctg tcgcgggagg
     3241 gcgaagcgac cggggtcgtg acgcatcggg ccatcgggtc gttgtatctg ccgctcttcc
     3301 tgcgtctgct gtgcggatcc gagcgcgccg gatcgatccg cagcgcatcg cggagcaagg
     3361 cgaacggatt tcccggagcg tcgtggatga ggacgccggt ctggctcgcc gacgacgggc
     3421 tcgcggtgat cgatcatcgt gtggccggtg acggcgtcga catcttcgtg agatccttcg
     3481 ggtccgacgg tcccttcctc gccgaacgca tggcggagcg gttgcgcacc tggcaggagg
     3541 gcgagggagg tcccgtcttc gaggcgatcc ccgtcgacgc tgtgcgaaag gcggcgccgg
     3601 agggcgtccg cgtgctgcgc cgcgggtcgt gggagttccg gctgtcctgg ctggcgtgac
     3661 cttccggatg cgcgggcatc cgcagcggag cgctgtgcga agacgcggcg cagaaggacg
     3721 gctgcaaaga aactcttgca aagaaacact tgcaaagaaa agcttgcata ggtatctttg
     3781 catctatgac agagcacgaa gaggtacgga cgctcgatgc cggagcgctc aaggcactcg
     3841 cacatccact gcgtgtgcgc atctacgatc tgctcagcga acgaggcccc cagaccgcga
     3901 gcagcctcgc ggcgctgatc ggcgagacct cgggggcgac gagctaccac ctgcgggcgc
     3961 tggcggccaa ggacctcatc cgcgaggtcg agggacgggg gacggcgcgc gagcggtggt
     4021 gggagcgccc gaaggggcgc atcaacctcc ccgggcccga cgacaggatg tcgcccgcca
     4081 accgggcagc ggcgcagatc gtcacctccg agttcttccg gctgcgtcac cagactctga
     4141 tggactacct caaccgtccg gactccgagc agccggagga atggcgggat gccggcctcg
     4201 tgatgacgac gaccctcgat ctcacccccg agcagatgtc cgagctcaag caggagctct
     4261 ccgacgcggt cgacgccgcc atcgagcgct atcgcggaca ggagggcgcg ccgggaaccc
     4321 ggcgcgtctc tctgcgcgcc gagatcttcg accttcccgc ggcgatcagc cgcggcacga
     4381 ccgaccagga gtgatcatga ccgccgcaac gcttcacctt gcttcaccga cgaccaccga
     4441 gcgcgctctg ctgaagatcg cggacgccat caccgcgtac accgagaacc gccgcctgct
     4501 gcgcgcggag cgccgggaaa tcgcactcga cctgctgcgc gagcagcagg cgcgcaagca
     4561 ggacccgcgg gctctcgagt acgcgatgct gtcgatcggc tcgcgcccgc gtcgttgacc
     4621 ggcggagcag acgagaagac cccggaccgc tgatcgcgtt ccggggtctc ctgcgtgtcg
     4681 ggctgcgcta gtcctcgaac ggtcgcgcga tcacgtcgcc ggatgccgtg agcaggacct
     4741 cccacccggc atccagctcc gcgaggggac ggccctcgag ccaggtcagg gtccagtggc
     4801 ccgtcagtcc tcgctcctcg acctcagcga tcgcagggcg aaggtcggca gggacggatg
     4861 caggcggctc cgaaccggtc gcccagcgcg tgccgagctg catgatcagg cctccaccgg
     4921 ggcgagctcg atcgcggtca ccgcgagctc ctcggcctcg gcgaagctca gctcggcgat
     4981 ccggcccaca gcgcgcagat cgtcggccgc cgcacgcagc gcctcgatct tcgccgccgg
     5041 tgccgcgatc gtcgcggtcg acaccggggt cttctgcgag gccttcgcct cggtcttggc
     5101 gcggcggat
//
LOCUS       DATMSX010000564         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_179590,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000564 DATMSX010000000
VERSION     DATMSX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179590"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..883)
                     /locus_tag="VN041_17915"
     CDS             complement(83..883)
                     /locus_tag="VN041_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU30961.1"
                     /translation="MSARTSRLRADNISLAYGDRRVVEGLSLAVPDSEITVIVGPNAC
                     GKSTVLRALARLISPASGAVLLDGRAIAEQPSRMVARTLGLLPQSPIAPEGITVRDLV
                     ARGRHPHQSWWRQWSEADAVAVDAALQATGMRELADRDVDELSGGQRQRAWIAMAVAQ
                     ETELLLLDEPTTYLDVAHQLDVLELVARLNSEHGRTIVMVLHDLNLASRYAHHMIAMR
                     EGAILAEGAPADVVTTDLLRDVFHVEGHVILDPHTGAPVVLPHRTIGD"
     gene            complement(880..>1547)
                     /locus_tag="VN041_17920"
     CDS             complement(880..>1547)
                     /locus_tag="VN041_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HWU30962.1"
                     /translation="IGVVPIPVAALVGGLLAAVVIAALASDRALRGRRILLVGIAVST
                     ALMGVTQYLLTAIDVNDAQRSTAWLTGSLNARGWDEAMPVAIGLCAALPVVLVLAHGG
                     RALLFGDDLATGWGVRTGRVRVVLLLTAVCLAALATSAAGPVAFVALASGQIARRIAG
                     VPDIPPILAAIVGAATVLVADTVARTALPYPVPVGVLTAVFGAPYLLHLLQQGRPRKG
                     APA"
BASE COUNT          258 a          546 c          548 g          195 t
ORIGIN      
        1 aggccccggt ccggtacgac ccgcgctgag cggggcaggc gcaccgggcg gcggcgccga
       61 cggcacctgc tcgacagacc cctcagtccc cgatcgtccg gtgcggcaac acgacgggcg
      121 cgcccgtgtg cggatcgagg atcacgtggc cctccacgtg gaagacgtca cggagcagat
      181 cggtcgtgac gacgtcggcc ggggcgccct cagcgaggat cgcgccctca cgcatcgcga
      241 tcatgtggtg tgcgtaccgg ctggcgaggt tcagatcgtg cagcaccatc acgatcgtgc
      301 ggccgtgctc gctgttcagc cgcgccacga gctccaacac gtcgagttga tgggcgacat
      361 cgagataggt ggtgggttcg tcaagcagca gcagctcagt ctcctgtgcg accgccatcg
      421 cgatccaggc gcgctgacgc tgaccgccgg acagctcgtc gacgtcgcgg tcggcgagct
      481 cgcgcatccc cgtcgcctga agcgctgcgt ccacggctac cgcatcggcc tcgctccatt
      541 gccgccacca ggactggtga gggtgacggc cgcgggcgac caggtcgcgc acggtgatcc
      601 cctcgggcgc gatcggcgac tgcggcagca gaccgagcgt ccgtgcgacc atgcgcgacg
      661 gctgctccgc gatggcgcgt ccgtccagca ggaccgcacc ggatgccggg gagatgagac
      721 gcgccagcgc gcgcagcacg gtcgacttac cgcaggcgtt cggaccgacg atgaccgtga
      781 tctcgctgtc cggcacggcg agggacaggc cctccacaac ccggcggtca ccgtaggcga
      841 gggagatgtt gtccgctcgc agacgcgatg tacgagcact cacgcgggtg ctcccttcct
      901 gggacgtccc tgctggagca ggtggagcag atagggtgcg ccgaagacgg cggtcagcac
      961 gccgacgggc accggatacg gcagtgcggt gcgagccacg gtgtcggcga cgaggaccgt
     1021 cgccgcaccc acgatcgcgg cgaggatcgg cggaatgtcg ggaacgccgg cgatcctcct
     1081 cgcgatctga ccggatgcca gtgcgacgaa cgccaccgga ccggccgcgg acgtcgcgag
     1141 cgcggcgagg cataccgcgg tgagcagcag cacgactcgc acccgaccag tgcgcacgcc
     1201 ccaaccggtg gcgaggtcgt ctccgaacag cagcgcacgc ccgccatgag caagcaccag
     1261 cacgacgggc aacgccgcac agagccctat cgcgacgggc atcgcctcat cccacccgcg
     1321 ggcgttgagg ctcccggtga gccaggcggt cgagcgctgc gcgtcgttca cgtcgatggc
     1381 ggtcaggagg tattgcgtca cgcccatcag tgccgtcgac acggcgatcc ccaccagcag
     1441 gatgcgtcgc ccgcggagtg cacgatcgct ggcgagggcc gcgatgacca ccgccgccag
     1501 cagaccgccg acgagcgcgg cgacggggat cggcaccacg ccgatcg
//
LOCUS       DATMSX010000565        11102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_75341,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000565 DATMSX010000000
VERSION     DATMSX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75341"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VN041_17925"
     CDS             <1..386
                     /locus_tag="VN041_17925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWU30963.1"
                     /translation="LRPEQLGGAAALASRHPDTAIVLCHLGLGHGEPDAEWLGGLTAF
                     AERPNAHAKFSGLLSPTRTDAGLADLADTALRLFRADRLMFGSDWPMSARTHSYADVV
                     AATRRALPHPDTTDFWAGTVTRLYG"
     gene            532..837
                     /locus_tag="VN041_17930"
     CDS             532..837
                     /locus_tag="VN041_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWU30964.1"
                     /translation="MPHVYILKCSDGSLYTGSTWSLELRVAQHESGEGCDYTRRRRPV
                     QLVWSAEFERVAEAYDWERRIHGWSRAKKQLLIDGRYEDLPGWSRRQRAAKRAEESG"
     gene            complement(923..1579)
                     /locus_tag="VN041_17935"
     CDS             complement(923..1579)
                     /locus_tag="VN041_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU30965.1"
                     /translation="MTDVMGAAGLQRGLLSDQIYAMIKAMIKDSSLAPGEQLVESQLA
                     RQLQVSQAPVRDALKRLAHEGLVSHVRHQGNFVASYSAEEAAQAKVARVELEALAGRL
                     ACGALDAERRGLLDDLIAGMHEAAESGDLARFRELDFAFHRGVIESSGNVYLPKMWDL
                     LEPSLRSLHVLGDPKFTGDWHEVADWHRGLLDVLEAGDPQAASDLFRAHAAGTLLDPA
                     "
     gene            complement(1599..2198)
                     /locus_tag="VN041_17940"
     CDS             complement(1599..2198)
                     /locus_tag="VN041_17940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWU30966.1"
                     /translation="MSFIGDSITDAGRDRTDPGSLGDGYVSLLAPELQAGGAEVLNLG
                     IAGDRAVDLAARWASDLMPTAPQVLTVYVGINDVWRRFDSDDPTPTDRFEETLRGLLA
                     SAVDAFDPALILMEPYLLPVRDEQRGWLEELDEKRGAVRRLAVEFGAQLVPLHEVLTA
                     AAEGRDAAELAPDGVHPFPEASRLIADAWRTAWAISDSR"
     gene            complement(2195..6259)
                     /locus_tag="VN041_17945"
     CDS             complement(2195..6259)
                     /locus_tag="VN041_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWU30967.1"
                     /translation="MKRALAAFAIAAVIGTALTATPASAADGPALYVAPGGDDAAAGT
                     IDAPLATLEGARDRIRQLKAGAGLPEGMTVFLREGQYDRSASFALGKEDSGTAEHPIV
                     YRSYPGEQATLTGGRELPREDFAKVTDSAVTGRIVDVAARDKVLEVDLKALGLNDYGQ
                     LSRHGYWKANDVSTTPPMELYIAGQGMTLARWPNADAATPTVQMGDIIDPGPTRKDAD
                     LQDRGGTFTYGYDRPKYWTEAKDVWLDGIFGYSWEWSYNKIEKIDTAAKTITLRYGEM
                     SGIMKSWFPDFHFAQNLLEELDAPGEYYIDRDAGKLYLMPNAAFTSGEGAVTVTTLSE
                     PMLRTDDTSYVTFDDIVMEYGRATAAVILGGSHVTVSHADIRNFTDGGVLINSPGRYT
                     YDGIPVNRGGRDHAVTDSRITHVGGIGVVLQGGDKTTLEPGRNRVENSEIADFAYYHK
                     AYNPGVMFDGVGNIAKNNKIHDAPHPGIIVYGNDHLFERNEVYDVCKMFNDLGAIYMN
                     SGKTPQQRGHVFRENYFHDIGVGIAGVEGIYADNFTWDLTIEKNVFANMGNSAIKSGS
                     ADYINASNNVFIDAYVPYDNYEQWMGNQEGNVVDRDYMPAWKQVFADNNDFVGTPYLE
                     KYPELSHFFEDDHYYPNHSTFAKNVVWNPNRKPIAGLNDQGAKDGKNLLNYGTNLVAT
                     ADPGFVDAAKGDYALKADSEVFSKIPGFEAIAFDKIGVSGHIGQSQSPDAVALKAIAF
                     DSDQLRIDAGDQARVRAVPLPWNAADSAVTYASADTAVATVDAKGLVLGMGPGTTTIT
                     ATAKSDPAKQATIEVVVSEGDGVLHFTDFQSGANGWPTDPNRSIQVDAAGNKVYRIVK
                     GANSLLPRDFTEFVLDFDVTPPAGDIATSGLVIYNRNQNGGGYIRYRNAAAGPTWDIF
                     DSNWKTVAEKVLPADQALPNGKTSHVRIAVQDGSIRIVVNGAIVLEGKDPAPGAAGRV
                     GFYVEGYASLDFDNIGFSLSGVPVTGIALDAEKLALNVGERHTVKATAAPEDASDTRV
                     SWSSADPAIATVGDDGRIAAVGGGTTTVTATSVSDPSLTASVQVTVADAAYPTVRLDR
                     QLTDAANWSTADGITVDADGVKLAAQGVYGYKAERFGSTLLKFRADFGKFDGGWYGFQ
                     VRSDQTGIPAWQNSNTGYLFIVKEDTFEFQSWTPGQTMLDVIPNTVIAPGSTHEIEVG
                     AVAEEGGARVVLRVDGKTVWNVRDARANLRIGADGFFNLYHYGKTNTFSVSAETQDAT
                     VTGICWSADAAPKTRYLRGEGLDTTGMLLDVAWSDGSSSQHQVTADMVSGFDSSKPDH
                     AQKLTVAYLGATTTLNITVRPKLKQEEVDVPRCG"
     gene            6589..7509
                     /locus_tag="VN041_17950"
     CDS             6589..7509
                     /locus_tag="VN041_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU30968.1"
                     /translation="MTELLNSSQPVAGRRPRRMSNGMFALLLTAPGLALLAAVVVYPL
                     ITALVTAFFKQSLVVPGREFVGLQNIIDVLTGDFPRLLGQTIVFTLGTTIAPFVIGFG
                     LALALNSRIRGAKVLRGIMLIPWLIPGVVVSFLWMWIFNANYGVMNALLQSIGLIDDP
                     QAWLAHPATAMIAVIVAKTWQSFPWMMVMLLAGLQTVPPELHEAAEIDGAGTVRRFFS
                     ITVPQLSGIIGLVLLLEFIWNFQHFDIIYVLTGGGPAGSTQTFATAVYETAFTGFDLG
                     HAGALGLLWMVLLMGLVVVYVRFSEKGEKR"
     gene            7506..8414
                     /locus_tag="VN041_17955"
     CDS             7506..8414
                     /locus_tag="VN041_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU30969.1"
                     /translation="MTALLTETPAVASDRPAPQQLRKPKMRADRRAVTISAWVAVVVF
                     GGFALLPVYWLLATSLTPRTEVFSYPPRLFPKQITFDAYLALLDNPALFGYLRNSIIV
                     SVITAVLSVIVSAYMGYAFSKFRYRGRRSLMYFVLASQMFPQALLLITLYTVFSAYGL
                     LNTYAALVLSFTTFTLPLCVWMLKGFFDTIPDDLIEAALVDGASRMRIIHSVVLPLAA
                     PGLVAAGLFAFVRGWNDFIFALTLAGPDKQTLPPGLVNTFIGEASTAWPELMAASLVV
                     SLPVAIAFIALQRFLVGGMTAGAVKG"
     gene            8473..9801
                     /locus_tag="VN041_17960"
     CDS             8473..9801
                     /locus_tag="VN041_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU30970.1"
                     /translation="MSQNTPSVSRRQLLQFAGLGAAGLMLAGCMPSGGTTGGGAPAAG
                     DAFAPGKFKATDFKFSSWSLTEEAAAPAIKALLEGYEKKSKVDITEVSFPYNEYFKQL
                     MLQVRGGQFSGAAHVDVAWLASLAALGKLQDVSAFTKGRGYTASALEAAQFDGKQYGF
                     PWTIGAIGLLTNSELIQKAGIDEFPVTVDEYEGALKKLKGLGNGLIPYAASTKAAQLK
                     DILIWMQTFGSDLVKDGKVTIGDDASIEAVTWYKSLYDQGLIAADVDRFDARSLFSQG
                     RTAIYDDAPVGRNSVIKDSPDAQLGEKIVSESRPVLKKGDTPRALVWGGAIVVVGGAS
                     DDSTGTAAEFGQWATSDLDAVLADYKVRALPPVTEKAQASDAVASDAFGSRFAEKITA
                     TATVNPFWQFPQYAQVETVVAERVQAVLVGQQSPKDAMAQAGEAAQKLIG"
     gene            9801..>11102
                     /locus_tag="VN041_17965"
     CDS             9801..>11102
                     /locus_tag="VN041_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HWU30971.1"
                     /translation="MGIPVTRRAVAGVTAGIAVALATALAPAASAGVHPSTVETVPVT
                     VDGSNQSGWWNPLAVVDGVTYFAYNVPAGTNRHEVHLASRTASGEWTSGCLRLATGAC
                     ANYLNDNGHNQPSVAVDGAGEIHVFASMHHEDWNYFRSTVPGDVTSLVEVSEEMPDQG
                     STISYPITAVGPDGDVWLMVRTGPDAQARRDGVLYRYDLDTRTWARETVVASATNYSV
                     YPDDVTVDGNGQVHLLWEWHAWAAGPFRHLGSYAVWNPVAKAFSDVAGGALPTPIRPT
                     TPGPVVFQPFTDGETMTDTTPAVQTAKMAIQDGVLRGVTYRYASGGSRLFDLEWASWN
                     GTDWSRETLIDPPSLGSGVDTVAAMDATSYGSKTRVYGVVTLQTCNVTRSQVVMFERA
                     DGRSDWAVEAVGDPMLGQQRLRSATRDDGTDVLYLSAPATPG"
BASE COUNT         1644 a         3840 c         3723 g         1895 t
ORIGIN      
        1 tgctgcggcc cgagcagctc ggcggcgccg ccgcgctggc atcccggcat ccggacacgg
       61 cgatcgtgct ctgccacctc gggctgggcc acggtgaacc ggacgctgaa tggctgggcg
      121 gtttgacggc gttcgccgag cggccgaacg cgcacgccaa gttctccggc ctgctgtccc
      181 cgacgaggac ggatgccggg ctcgcagacc tcgccgacac ggcgctccgg ctgttccgcg
      241 ccgaccggct gatgttcggc agcgactggc cgatgtcggc tcgcacgcat tcctatgccg
      301 acgtcgtcgc cgccacccgg cgggcgctgc cccaccccga caccaccgac ttctgggccg
      361 gcaccgtcac gcgcctgtac ggctgagcgc ccgagacgaa ggggcgccgt caggaccgca
      421 acaccccggt ccctgagcga gcgaagcgag acgaagggcg ctcaggaacc gacgaagggc
      481 gcctgtggat aaccgcggtc gcctgtcggc cgatcggcct agcgtcgggt catgccacac
      541 gtctacatcc tcaagtgctc cgacgggagc ttatacaccg ggagcacctg gagcctcgag
      601 cttcgagtcg ctcagcacga gagcggagag ggatgcgact acacgcggcg acgacgcccc
      661 gttcagctcg tctggagcgc ggagttcgaa cgtgtcgcgg aggcgtacga ctgggagcgc
      721 cggatccatg gctggtcgcg agcgaagaag cagctactca tcgacgggcg gtacgaggat
      781 cttccgggtt ggagcagacg gcagcgtgca gcgaagcgcg ccgaggaatc cggctgacgc
      841 ccttcatctc gggcgcgtag gcgcccttcg tctcgtcgct gcgctcctcg ctcaggggcc
      901 gggtcaggtc cttgagcgca cttcaggcgg ggtcgaggag ggtacccgcg gcgtgggcgc
      961 ggaacaggtc ggacgcggcc tgcggatcgc cggcctcgag cacgtcgagc agaccgcggt
     1021 gccagtcggc gacctcgtgc cagtcgccgg tgaacttcgg gtcgccgagc acgtgcagcg
     1081 aacgcaggct gggctccagc agatcccaca tcttgggcag gtagacgttc ccggatgact
     1141 cgatcacgcc ccggtggaac gcgaagtcca gctcgcggaa acgggcgaga tcgccggact
     1201 cggcggcctc gtgcatcccg gcgatgaggt cgtcgagcag gccgcggcgc tcggcgtcca
     1261 gcgccccgca cgcgagccgg ccggcgagcg cctccagctc cacgcgcgcg accttggcct
     1321 gcgcggcctc ctccgccgag tagctggcga cgaagttgcc ctggtgacgc acgtgagaga
     1381 ccaggccctc gtgcgcgagc cgcttgagcg catcgcgcac cggcgcctgg ctgacctgca
     1441 gctggcgcgc cagctgcgac tcgaccagct gctcaccagg ggccagcgag gagtccttga
     1501 tcatcgcctt gatcatggcg tagatctgat ccgagagcag tccccgctgc aggcctgctg
     1561 cgcccatcac gtccgtcata gcttccattg tactttcatt atcgactatc gctaatggcc
     1621 cacgcggtgc gccaggcatc ggcgatcagg cgcgaggcct ccgggaacgg atgcacaccg
     1681 tcaggggcca gctcagcggc atcccgcccc tcggccgctg cggtgagcac ctcgtgcagc
     1741 ggtaccagct gcgctccgaa ctcgacggcg agccgccgga cggctcctcg cttctcgtcg
     1801 agctcctcca gccagccccg ctgctcatcc cgcaccggaa gcagatacgg ctccatcagg
     1861 atcagcgcgg ggtcgaaggc gtcgacggcg gatgccagaa gcccccgcag cgtctcctcg
     1921 aaccggtccg tgggtgtcgg gtcgtcgctg tcgaaccggc gccacacgtc gttgatgccg
     1981 acgtagacgg tgagcacctg cggcgcggtg ggcatcagat ccgatgccca ccgcgccgcg
     2041 agatccacgg cccggtcacc ggcgatgccg aggttgagca cctcggcacc gccggcctgc
     2101 agctcgggcg ccagcagcga cacgtacccg tctccgagcg atccggggtc ggtcctgtcg
     2161 cgtccggcat ccgtgatgga gtcgccgatg aagctcatcc gcatcgcggc acgtccactt
     2221 cctcctgctt gagcttgggg cggaccgtga tgttcagcgt ggtcgtcgcg cccaggtagg
     2281 cgacggtcag cttctgcgcg tgatccggct tgctgctgtc gaatccgctc accatatcgg
     2341 cagtcacctg atgctgcgag ctgctgccgt cactccaggc gacatccagg agcatcccgg
     2401 tggtgtcgag gccctctccg cgcaggtaac gggtcttggg tgcagcatcc gccgaccagc
     2461 agatcccggt gacggtggca tcctgcgtct ccgcactcac cgagaaggtg ttggtcttgc
     2521 cgtagtggta caggttgaag aagccgtcgg cgccgatgcg caggttcgcc ctggcgtccc
     2581 gcacgttcca cacggtcttg ccgtcgaccc tcagcaccac gcgggcgccg ccctcctccg
     2641 ccacggcgcc gacctcgatc tcgtgcgtgc tgccgggcgc gatcaccgtg ttggggatga
     2701 catcgagcat ggtctgaccg ggggtccagc tctggaactc gaacgtgtcc tccttcacga
     2761 tgaacaggta gccggtgttg gagttctgcc aggccgggat gccggtctgg tccgagcgca
     2821 cctggaatcc gtaccagccg ccgtcgaact tgccgaagtc cgcgcggaac ttcagcagcg
     2881 tcgacccgaa gcgctcggcc ttgtagccgt acacgccctg cgcggcgagc ttcacgccgt
     2941 ccgcgtcgac ggtgatcccg tcagcggtgc tccagttcgc ggcatccgtc agctgccggt
     3001 cgagccgcac cgtcgggtag gcggcgtcgg ccacggtgac ctgcacggat gcggtgagcg
     3061 acgggtccga gacggaggtc gcagtcacgg tggtcgtgcc gcccccgacc gccgcgatgc
     3121 ggccgtcgtc cccgaccgtc gcgatcgcgg ggtcggcact ggaccagctc acgcgggtgt
     3181 cactcgcgtc ctcgggcgcg gccgtggcct tgaccgtgtg ccgctcgccg acgttcagcg
     3241 ccagcttctc ggcatccagg gcgatgccgg tgaccggcac ccccgagagc gagaacccga
     3301 tgttgtcgaa gtcgagcgag gcgtagccct cgacgtagaa gcccactcgg cccgcagccc
     3361 cgggcgccgg atccttgccc tcgagcacga tcgcaccgtt cacgacgatc cggatcgagc
     3421 cgtcctgcac cgcgatgcgc acgtgcgagg tcttcccgtt cgggagcgcc tgatcggccg
     3481 gcagcacctt ctcggcgacg gtcttccagt tgctgtcgaa gatgtcccag gtcggccccg
     3541 cggcggcgtt gcggtagcgg atgtacccgc ctccgttctg attgcggttg tagatgacca
     3601 gacccgaggt ggcgatgtcg ccggcgggcg gggtgacgtc gaagtccagc acgaactcgg
     3661 tgaagtcgcg cgggagcagg gagttcgcgc ccttcacgat ccggtacacc ttgttgccgg
     3721 cggcatcgac ctggatgctg cggttcgggt cggtcggcca tccgttcgca cccgactgga
     3781 agtcggtgaa gtgcagcacg ccgtcgccct cggagacgac gacctcgatg gtcgcctgct
     3841 tcgcggggtc ggacttcgcg gtcgccgtga tggtcgtcgt tcccgggccc atgccgagca
     3901 ccaggccctt ggcgtccacc gtggccacgg ccgtgtcggc cgaggcgtag gtcacggcgg
     3961 agtcggccgc gttccacggc agcggcacgg cgcgcacccg cgcctggtcg ccggcgtcga
     4021 tgcgcagctg gtcgctgtcg aaggcgatgg ccttcagcgc cacggcgtcc ggggactgcg
     4081 actgcccgat gtgcccggac acgccgatct tgtcgaacgc gatcgcctcg aacccgggga
     4141 tcttcgagaa gacctcggag tcggccttga gggcgtagtc gcccttggcg gcgtccacga
     4201 aaccggggtc ggcggtggcg accaggttgg tgccgtagtt cagcaggttc ttgccgtcct
     4261 tcgcgccctg gtcgttcagc ccggcgatgg gcttgcggtt ggggttccac accacgttct
     4321 tcgcgaaggt gctgtggttc gggtagtagt ggtcgtcctc gaagaagtga ctcagttcgg
     4381 ggtacttctc caggtacggg gtgccgacga agtcgttgtt gtcggcgaac acctgcttcc
     4441 aggccggcat gtagtcgcgg tcgacgacgt tgccctcctg gttccccatc cactgctcgt
     4501 agttgtcata cggcacgtag gcgtcgatga agacgttgtt gctggcgttg atgtagtcgg
     4561 ccgagccgct cttgatggcg gagttgccca tgttcgcgaa cacgttcttc tcgatggtga
     4621 ggtcccacgt gaagttgtcg gcgtagatgc cctcgacccc tgcgatgccc acgccgatgt
     4681 catggaagta gttctcgcgg aagacgtgtc cgcgctgctg cggggtcttg ccggagttca
     4741 tgtagatcgc gccgagatcg ttgaacatct tgcagacgtc gtacacctcg ttgcgctcga
     4801 acaggtggtc gttgccgtag acgatgatgc ccggatgcgg ggcgtcgtgg atcttgttgt
     4861 ttttggcgat gttgccgacc ccgtcgaaca tcacacccgg gttgtaagcc ttgtggtagt
     4921 acgcgaagtc ggcgatctcg gagttctcga cccggttgcg tcccggctcg agcgtggtct
     4981 tgtcaccgcc ctgcagcacc acgccgatgc cgccgacgtg ggtgatccgg gagtcggtca
     5041 cggcgtggtc gcggccgccg cggttcacgg ggatgccgtc gtaggtgtag cggccgggag
     5101 agttgatcag cacgccgccg tcggtgaagt tgcggatgtc ggcgtgcgac accgtcacgt
     5161 gcgaaccgcc gaggatgacg gcggccgtgg cgcgcccgta ctccatcacg atgtcgtcga
     5221 aggtgacgta cgaggtgtcg tcggtgcgca gcatcggctc gctgagcgtg gtcaccgtca
     5281 cggcgccctc acccgaggtg aacgccgcgt tgggcatgag gtacagcttt ccggcgtccc
     5341 ggtcgatgta gtactcgccc ggggcgtcca gctcctccag caggttctgc gcgaagtgga
     5401 agtcggggaa ccagctcttc atgatcccgg acatctcgcc gtagcgcagc gtgatggtct
     5461 tggccgccgt gtcgatcttc tcgatcttgt tgtacgacca ctcccagctg tagccgaaga
     5521 tgccgtccag ccagacgtcc ttcgcctcgg tccagtactt cgggcggtcg tagccgtagg
     5581 tgaacgtgcc gccgcggtcc tgcaggtcgg catccttgcg ggtgggaccg ggatcgatga
     5641 tgtcacccat ctgcacggtg ggggtcgcgg cgtcggcgtt gggccagcgc gccagcgtca
     5701 tgccctgacc tgcgatgtag agctccatcg ggggcgtggt gctgacgtcg ttggccttcc
     5761 agtagccgtg ccggctgagc tggccgtagt cgttcaggcc gagcgccttc agatcgacct
     5821 cgagaacctt gtcacgtgcg gcgacgtcga cgatgcggcc ggtgaccgcg ctgtcggtca
     5881 ccttcgcgaa gtcctcgcgc ggcagctcgc ggccgcccgt cagggtcgcc tgctcgcccg
     5941 ggtaggagcg gtagacgatc ggatgctcgg ccgtgccgga gtcctccttg ccgagcgcga
     6001 acgaggcgct gcggtcgtat tgcccttcgc gcaggaacac ggtcatgccc tcgggcaggc
     6061 ccgcgcccgc cttgagctgg cggatgcgat cacgcgcacc ttcgagtgtg gcgagtgggg
     6121 cgtcgatggt tcccgcggcg gcgtcatcac cccctggcgc cacgtagagg gcgggcccgt
     6181 cggcggcgga tgccggggtg gcggtcagcg ccgtcccgat caccgccgct atggcgaatg
     6241 ctgccagtgc acgcttcatc gcgtctccgt ctgctcgtgg tgagagttgc tcttcatgag
     6301 ggttgcgtcg agggactgcg cctcgaggcc ggctggaggc ccgcccgatc gctggggcga
     6361 tcgatcgaga gcctagctaa tcgataatcg ctaatcaagc atcgatctgg cgtgggatca
     6421 cgcccgtgac gactcgacac attttctaac gtgttttaac atgttctctc gttactcgat
     6481 cgtgacgacg cgaggttgac gaaacgggcg atcaccggaa acactggaaa ctccgcgccg
     6541 caacacccgg atgtcgcgga gagcccgaag gtcaaggatg ccccagtcat gacagaactt
     6601 ctcaacagtt cacagccagt agcaggacga cgcccccggc gaatgtcgaa cggcatgttc
     6661 gctctgctgc tgacggcacc cggtctggcg ctgctggccg cggtggtcgt ctacccgctg
     6721 atcaccgcgc tcgtgaccgc gttcttcaag cagagcctgg tcgtgcccgg ccgcgagttc
     6781 gtcggcctgc agaacatcat cgacgtgctc accggcgact tcccccgcct gctcggccag
     6841 acgatcgtct tcacactcgg caccaccatc gcgccgttcg tgatcggctt cggtctggcg
     6901 ctcgcgctga actcccgcat ccggggcgcg aaggtgctgc gcggcatcat gctcatcccc
     6961 tggctgatcc ccggcgtcgt cgtgtcgttc ctgtggatgt ggatcttcaa cgccaactac
     7021 ggcgtgatga acgccctgct gcagtccatc ggcctcatcg acgacccgca ggcctggctg
     7081 gcgcacccgg ccaccgccat gatcgccgtg atcgtcgcca agacgtggca gtcgttcccc
     7141 tggatgatgg tcatgctgct ggccggtctg cagaccgttc cgcccgagct gcacgaggcc
     7201 gctgagatcg acggcgccgg cacggtccgc cgcttcttct cgatcaccgt gccgcagctg
     7261 agcggcatca tcggcctggt gctgctgctc gagttcatct ggaacttcca gcacttcgac
     7321 atcatctacg tgctcaccgg cggaggaccg gccggctcca cccagacctt cgccacggcc
     7381 gtgtacgaga ccgcgttcac cgggttcgac ctcggccacg ccggtgccct cgggctgctc
     7441 tggatggtgc tgctgatggg tctcgtcgtc gtctacgtcc gattctcgga gaagggagag
     7501 aagcgatgac cgcgctgctc accgagacgc ccgctgtggc atccgaccgt cccgcgccgc
     7561 agcagctgcg caagccgaag atgcgcgccg accgtcgcgc cgtcacgatc agcgcctggg
     7621 tggccgtcgt cgtcttcggc ggcttcgcac tgctgcccgt gtactggctg ctggcgacct
     7681 cgctcacccc gcgcaccgag gtgttctcgt acccgccgcg cctgttcccg aagcagatca
     7741 cgttcgacgc gtacctggcg ctgctcgaca accccgcgct cttcgggtat ctgcgcaaca
     7801 gcatcatcgt gtcggtgatc acggccgtgc tctcggtgat cgtctcggcg tacatgggct
     7861 acgcgttctc gaagttccgc taccgcgggc gccgcagcct gatgtacttc gtgctggcct
     7921 cgcagatgtt cccgcaggcc ctgctgctga tcaccctgta cacggtgttc tctgcctacg
     7981 gcctgctgaa cacctatgcc gcactggtgc tgtcgttcac caccttcacg ctgccgctgt
     8041 gcgtgtggat gctgaagggc ttcttcgaca cgatccccga cgacctgatc gaggccgccc
     8101 tcgtcgacgg cgcctcgcgg atgcgcatca tccactccgt ggtgctgccc ctcgccgcgc
     8161 ccggtctggt cgccgccggc ctgttcgcct tcgtccgcgg ctggaacgac ttcatcttcg
     8221 cgctgaccct cgccggcccc gacaagcaga ccctgccccc gggcctggtg aacacgttca
     8281 tcggcgaggc ctccaccgcc tggcccgagc tgatggccgc ctcgctcgtg gtctccctgc
     8341 cggtggccat cgcgttcatc gccctgcagc gcttcctcgt cggcggcatg accgccggcg
     8401 cggtcaaggg ctgaccccgc ccgctccgac accctcccac gcagaacccc ctcgaagaag
     8461 agagagaaaa ccatgtctca gaacaccccc tccgtctcgc gccgccagct cctgcagttc
     8521 gcaggactgg gagccgcggg actgatgctg gccggctgca tgcccagcgg cggcaccacc
     8581 ggcggaggcg cccccgccgc gggtgacgcg ttcgcccccg gcaagttcaa ggccaccgat
     8641 ttcaagttct ccagctggtc gctgaccgag gaggccgcgg ccccggcgat caaggcactc
     8701 ctggagggct acgagaagaa gagcaaggtc gacatcaccg aggtctcgtt cccctacaac
     8761 gagtacttca agcagctcat gctgcaggtg cgcggcggcc agttctccgg cgccgcccac
     8821 gtcgacgtcg cctggctcgc ctcgctggcg gccctgggca agctgcagga cgtctcggcc
     8881 ttcaccaagg gccgcggcta cacggcatcc gctctggagg ccgcgcagtt cgacggcaag
     8941 cagtacggct tcccgtggac gatcggtgcg atcggcctgc tcaccaactc cgagctgatc
     9001 cagaaggcgg gcatcgacga gttccccgtc acggtcgacg agtacgaggg cgcgctgaag
     9061 aagctcaagg gtctcggcaa cgggctcatc ccctacgccg cgtcgaccaa ggcggcgcag
     9121 ctgaaggaca tcctcatctg gatgcagacc ttcggcagcg acctggtgaa ggacggcaag
     9181 gtcaccatcg gcgacgacgc cagcatcgag gccgtcacct ggtacaagtc cctgtacgac
     9241 cagggcctga tcgccgctga tgtcgaccgc ttcgacgccc gctcgctgtt ctcgcagggg
     9301 cgcacggcga tctatgacga cgccccggtc ggccgcaact cggtgatcaa ggactcgccg
     9361 gacgcccagc tcggcgagaa gatcgtgtcg gagtcgcgcc cggtgctgaa gaagggcgac
     9421 accccgcgcg ccctcgtctg gggcggtgcg atcgtggtgg tcggcggtgc gagcgacgac
     9481 tccacgggca ccgccgcgga gttcgggcag tgggccacca gcgatctgga tgccgtgctg
     9541 gccgactaca aggtgcgcgc cctgcctcct gtgaccgaga aggcgcaggc ctccgacgcg
     9601 gtcgcctcgg acgccttcgg ctcgcgcttc gcggagaaga tcactgccac cgccacggtg
     9661 aacccgttct ggcagttccc gcagtacgcc caggtcgaga ccgtggtcgc cgagcgcgtg
     9721 caggcggtgc tggtcggcca gcagagcccg aaggacgcca tggcccaggc gggcgaggcc
     9781 gcacagaagc tgatcggctg atgggcatcc cggtcacgcg ccgcgcagtg gcaggggtga
     9841 cggcgggcat cgccgtcgcc ctggccaccg ctctggcgcc cgcggcgagc gcgggcgtgc
     9901 acccgagcac ggtcgagacc gtgccggtga ccgtcgacgg gtccaaccag tccggctggt
     9961 ggaatccgct ggccgtcgtc gacggggtca cgtacttcgc ctacaacgtg cccgcgggca
    10021 cgaaccgcca cgaggtgcat ctcgcctcgc gtaccgcgtc gggcgagtgg acctccggat
    10081 gcctgcggct ggccaccggc gcgtgcgcga actacctgaa cgacaacggg cacaatcagc
    10141 cctcggtcgc ggtggacggc gcgggcgaga tccacgtgtt cgcctccatg caccacgagg
    10201 actggaacta cttccgctcc accgtgcccg gcgacgtcac ctcgctggtc gaggtgagcg
    10261 aggagatgcc cgaccagggc tcgacgatct catacccgat caccgcggtc ggcccggacg
    10321 gcgacgtctg gctgatggtg cgcaccgggc ccgacgccca ggcgcgccgc gacggcgtgc
    10381 tctaccgcta cgacctggac acgcgcacct gggcgcgcga gaccgtggtc gcctcggcga
    10441 cgaactactc ggtgtacccc gacgacgtga ccgtcgacgg gaacgggcag gtgcacctgc
    10501 tctgggagtg gcacgcctgg gccgccggcc ccttccgtca cctgggctcg tacgccgtgt
    10561 ggaaccccgt cgcgaaggcg ttcagcgatg tcgccggcgg ggcgctcccc acgccgatcc
    10621 ggccgaccac ccccggcccg gtggtcttcc agccgttcac cgacggcgag accatgaccg
    10681 acaccacccc ggccgtgcag accgccaaga tggcgatcca ggacggcgtg ctgcggggcg
    10741 tgacgtaccg ctacgcctcg ggcggctcgc gcctgttcga cctggagtgg gcgagctgga
    10801 acggcaccga ctggagccgc gagacgctca tcgacccgcc gtccctcgga tccggagtcg
    10861 acaccgtggc cgcgatggat gccacctcgt acggttcgaa gacccgcgtg tacggcgtcg
    10921 tgacgctgca gacctgcaac gtcacgcgca gccaggtcgt catgttcgag cgcgccgacg
    10981 gccgctcgga ctgggcggtc gaggccgtcg gagacccgat gctcggccag cagcgactgc
    11041 gctccgcgac ccgcgacgac ggcaccgatg tgctgtacct cagcgccccg gccacgccgg
    11101 gc
//
LOCUS       DATMSX010000566         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_17914,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000566 DATMSX010000000
VERSION     DATMSX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17914"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..911
                     /locus_tag="VN041_17970"
     CDS             285..911
                     /locus_tag="VN041_17970"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWU30972.1"
                     /translation="MATYTLPDLPYDFAALEPHISGKIMELHHDKHHAAYVAGANAAL
                     DALAEARDSGNLANVNKLEKDLAFNLGGHVNHSIFWTNLSPNGGGQPEGELKAAIDEF
                     FGSFEKFQAHFAAAAAGIQGSGWAVLSWDPIGARLIIQQLFDQQANTAQGTVPLFQLD
                     MWEHAFYLDYLNVKADYIKAAWNIANWENVAQRFETAREKTSGLLVLS"
     gene            1012..2022
                     /gene="gap"
                     /locus_tag="VN041_17975"
     CDS             1012..2022
                     /gene="gap"
                     /locus_tag="VN041_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWU30973.1"
                     /translation="MSVKIGINGFGRIGRNFLRAALAQNADLEVVAVNDLTDNAALAH
                     LLKYDSVGGPLGVEVSHEGDVITVGGKAIKVFEERDPANLPWGELGVDIVIESTGRFT
                     KAADAAKHIAGGAKKVIISAPGTDVDGTFVMGVNEDTYDAANHNIISNASCTTNCLAP
                     LAKVFNDTFGIERGFMMTAHAYTADQNLQDGPHSDLRRARAAAINIVPASTGAAKAIG
                     LVLPELNGKLSGSSYRVPVPTGSIVDLTIISDRDDLTVDEINAAYREAAAEGRLNGIL
                     EYTSDEIVSSDIQLNAHSSIFDSGLTNVSGNLVKVSAWYDNEWGYSNRLVDLTEYVAE
                     RL"
     gene            2033..3247
                     /locus_tag="VN041_17980"
     CDS             2033..3247
                     /locus_tag="VN041_17980"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWU30974.1"
                     /translation="MTLRTLESLGSLEGRRVIVRCDLNVPLRDGVITDDGRVRASLPT
                     LNALINSGARVIVCSHLGRPDGAPDPQYSLEPVAQRLSELLGKPVAFARDTVGESARE
                     AVSSLEDGGVVVLENLRFNAGETAKDDATRQAFARELAELGDALVSDGFGVVHRKQAS
                     VYDLAQILPSAAGLLIATELDVLDRLTENPERPYTVVLGGSKVSDKLGVISHLLPRVD
                     RLLVGGGMLFTFLAAEGHGVASSLLEKDQLDTVRGYIAEAKERGVELVLPTDVVVASG
                     FAADAEHETVAADAIEQSSFGASGIGLDIGPDTAARFADAIRDSKTVFWNGPMGVFEF
                     PAFAAGTKAVASALTEVDGLGVVGGGDSAAAVRQLGFSDDQFGHISTGGGASLEFLEG
                     KKLPGLEVLGWQ"
     gene            3238..4029
                     /gene="tpiA"
                     /locus_tag="VN041_17985"
     CDS             3238..4029
                     /gene="tpiA"
                     /locus_tag="VN041_17985"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWU30975.1"
                     /translation="MAMTSRTPLIAGNWKMNLDHLQAVATVQKLHWTLKDAGHENGSV
                     EVAVFPPFTDIRSVQTLIDADKIPFGLGAQDLSAHDSGAYTGEISGAFLSKLGVGSVI
                     IGHSERREYHQESDELVNAKVKAALKHGLVPVICVGETAEDLERFGSSAVPVGQLEKA
                     LEGVPASADIVVAYEPVWAIGSGQAATPAQAQEVCAALRAVIARVLDDEAAARTRILY
                     GGSVKSGNIASFMREPDVDGALVGGASLVVDEFAAIVRFQKHVGV"
     gene            4113..4361
                     /gene="secG"
                     /locus_tag="VN041_17990"
     CDS             4113..4361
                     /gene="secG"
                     /locus_tag="VN041_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWU30976.1"
                     /translation="MLVLEFVLQVLLGISSVLLTLLILLHKGRGGGLSDMFGGGMSAA
                     VGSSGLAERNLNRFTVILALVWFVSIVALGLITKFSVI"
     gene            4361..4717
                     /locus_tag="VN041_17995"
     CDS             4361..4717
                     /locus_tag="VN041_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HWU30977.1"
                     /translation="MATGGNAIRGTRVGSGPMGEQDHGYHADRIAVSYWDGLGNETVR
                     YFAAGLPEEEIPDTIDHPHTGLPAGRDRQNPPALAKAEPYKTHLAYVKERRTDEEAEQ
                     LLEDALTQLRERRGQR"
     gene            complement(4811..>5281)
                     /locus_tag="VN041_18000"
     CDS             complement(4811..>5281)
                     /locus_tag="VN041_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30978.1"
                     /translation="GLLCLTGVLLPGLRTTEAAWTADAVTNTTVTAVPAPTTATGATC
                     KPTSAIVVGLQRVDLAWSSTLPLADQRVDITKNSTTARDTSQITLTGQTGGVYSYAAT
                     YDTAKLLGLLNLSDLLGGTYGISIQTGYPNTTWYAPPKTFTLNVILLGLGSTCS"
BASE COUNT          863 a         1803 c         1769 g          846 t
ORIGIN      
        1 atccgccgcc tcctgaccat ggccgacaag cgtgcccagc aggacggcat ccccggtacc
       61 gaggcggccg tcccggccgg actcgaggac tagcgggaac tggccaggcg gcccctgccg
      121 acgcgttcga cacgcgcggg caggggccgt tctcgtcatc cggggaaggt tccctccgcc
      181 atcacggttg ccctcagtag gatgtgaaac gtctgcttcg cggcgcacac ggcgccgtgg
      241 agaacgcaag cgccgcggcg cggcgcggat tggatgaaat cgacatggcg acttatacgc
      301 tgcccgacct tccctacgac ttcgcggctc tggagcccca catcagtggc aagatcatgg
      361 agcttcacca tgacaagcac cacgccgcct acgtcgccgg cgccaacgcc gccctcgacg
      421 cactcgccga ggcccgcgac tccggcaacc tggccaacgt gaacaagctc gagaaggacc
      481 tggcgttcaa cctcggtggt cacgtcaacc actccatctt ctggacgaac ctgtcgccga
      541 acggcggcgg tcagcccgag ggtgagctga aggccgccat cgacgagttc ttcggctcgt
      601 tcgagaagtt ccaggcccac ttcgcggctg cggctgccgg catccagggc tccggctggg
      661 ccgtgctgag ctgggacccg atcggcgcgc gcctgatcat ccagcagctg ttcgaccagc
      721 aggccaacac cgctcagggc acggtgccgc tgttccagct cgacatgtgg gagcacgcct
      781 tctacctcga ttacctgaac gtcaaggcgg actacatcaa ggcggcgtgg aacatcgcca
      841 actgggagaa cgtcgcgcag cggtttgaaa cagcgcgtga gaagacctcc ggtctgctgg
      901 tactgtcgta aaagatgggc gtcctgatgg gcgatttgcc catcaggacg ccgttgtccg
      961 cgcaatccgc gaaaattacg caattccctt gttaagaacc aggagacttt cgtgtctgtc
     1021 aagatcggta tcaacggctt cggtcgtatc ggaagaaact tcctccgcgc agctctcgcg
     1081 cagaacgctg acctcgaggt cgtcgctgtc aacgacctca ccgacaacgc ggcactcgcc
     1141 catctcctga agtacgactc ggtcggcggc cccctgggcg tcgaggtctc gcacgagggc
     1201 gatgtcatca ccgtcggcgg caaggccatc aaggtcttcg aagagcgcga ccccgccaac
     1261 ctcccgtggg gcgagctggg cgtggacatc gtcatcgagt cgaccggacg cttcaccaag
     1321 gctgctgacg ccgccaagca catcgccggt ggcgccaaga aggtcatcat ctccgcgccg
     1381 ggcaccgacg tcgacggcac cttcgtcatg ggtgtgaacg aggacaccta cgacgcggcg
     1441 aaccacaaca tcatctccaa cgcgtcctgc accacgaact gcctcgcgcc gctcgcgaag
     1501 gtgttcaacg acaccttcgg catcgagcgc ggcttcatga tgaccgcaca cgcgtacacg
     1561 gctgaccaga atctgcagga cggcccgcac agcgacctgc gtcgcgcccg cgccgccgcg
     1621 atcaacatcg tcccggcctc gaccggcgcc gccaaggcca tcggcctggt cctccccgaa
     1681 ctgaacggca agctcagcgg ctcctcctac cgcgtcccgg ttcccaccgg ctcgatcgtg
     1741 gacctgacga tcatctccga ccgcgacgac ctgaccgtcg acgagatcaa cgccgcgtac
     1801 cgcgaggccg ctgcggaggg ccgcctgaac ggcatcctgg agtacacctc ggacgagatc
     1861 gtgtcgagcg acatccagct caacgcgcac tcctccatct tcgactccgg gctgaccaac
     1921 gtcagcggca acctggtcaa ggtctccgcc tggtacgaca acgagtgggg ctactccaac
     1981 cgcctggtcg acctgaccga gtacgtggca gagcgcctct aagctcatac acatgactct
     2041 gcgcaccctg gaatctctgg gttccctcga gggcaggcgc gtcatcgtcc gttgtgacct
     2101 caacgtcccc ctgcgggacg gggtcataac ggacgatggt cgtgttcggg cctcgttgcc
     2161 gaccctcaac gccctcatca actccggcgc acgcgtcatc gtgtgctcgc acctgggccg
     2221 tcccgacggg gcgcccgacc cgcagtacag cctggagccg gtggcccagc ggctgtcgga
     2281 gctgctcggc aagcccgtgg ccttcgcccg cgacaccgtg ggcgagtccg cacgtgaggc
     2341 ggtctcctcg ctggaggacg gcggggtcgt cgtcctcgag aacctgcgtt tcaacgcggg
     2401 ggagacggcg aaggacgacg cgacgcgtca ggccttcgcc cgtgagctcg cggagctcgg
     2461 cgacgcactc gtctcggacg gattcggcgt cgtgcaccgc aagcaggcca gcgtgtacga
     2521 cctcgcgcag atcctgccgt cggccgccgg tctgctcatc gcgaccgagc tcgatgtgct
     2581 cgaccgtctg accgagaacc ctgagcgccc gtacacggtg gtgctcggcg gctcgaaggt
     2641 cagcgacaag ctgggcgtca tctcgcacct gctgccgcgc gtggaccggc tgctggtcgg
     2701 cggtgggatg ctgttcacct tcctggcggc ggagggccac ggcgtggcat ccagccttct
     2761 cgagaaggat cagctcgaca cggtccgcgg gtacatcgcc gaggccaagg agcgcggggt
     2821 ggagctggtg ctcccgaccg acgtggtcgt cgcgtccggc ttcgcggcgg atgccgagca
     2881 cgagacggtc gccgcggacg ccatcgagca gagctcgttc ggcgcatccg gcatcggcct
     2941 ggacatcggc ccggacaccg cggcgcgctt cgccgacgcg atccgcgact cgaagacggt
     3001 gttctggaac ggcccgatgg gcgtgttcga gttccccgcc ttcgccgcgg gtacgaaggc
     3061 ggtcgcctcg gcgctgaccg aggtcgacgg tctcggcgtc gtcggcggcg gcgactccgc
     3121 cgcggccgtc cgtcagctcg ggttctccga cgatcagttc ggacacatct cgacaggcgg
     3181 cggtgcaagc ctcgagttcc tcgagggcaa gaagctaccc ggactggagg tcctcggatg
     3241 gcaatgacga gccggacccc gctgatcgcg gggaactgga agatgaacct cgaccacctg
     3301 caggcggtcg ccaccgtgca gaagctgcac tggacgctga aggacgccgg gcacgagaac
     3361 gggtccgtcg aggtggcggt gttcccgccg ttcaccgaca tccgatcggt gcagaccctg
     3421 atcgacgcgg acaagattcc gttcggtctc ggcgcgcagg acctctccgc gcacgactcg
     3481 ggcgcataca ccggcgagat ctcgggcgcg ttcctgtcca agctgggcgt gggctcggtg
     3541 atcatcgggc actccgagcg ccgtgagtat caccaggaga gcgacgagct cgtgaacgcc
     3601 aaggtgaagg cggcgctgaa gcacggcctg gtgccggtga tctgcgtcgg cgagaccgcc
     3661 gaggacctgg agaggttcgg cagcagcgcc gtccccgtgg gccagctgga gaaggcgctc
     3721 gagggtgtgc cggcatccgc cgacatcgtc gtcgcctacg agccggtctg ggcgatcggc
     3781 tccgggcagg cggccactcc cgctcaggcg caggaggtct gcgcggcact gcgtgcggtc
     3841 atcgcccgcg tcctggacga cgaggcggcc gcgcgcaccc gcatccttta cggcgggtcg
     3901 gtgaagtccg gcaacatcgc cagcttcatg cgcgagccgg acgtggacgg cgcgctggtg
     3961 ggcggtgcga gcctggtcgt cgacgagttc gcggccatcg tgcgcttcca gaagcacgtc
     4021 ggcgtgtgag tcggtggggc gccggcggcg ccccaccgta taatcgacag ctaggcgggt
     4081 cgtttccgac cctggcgaaa ggcttcaatc tcgtgcttgt tctcgagttc gtgctgcagg
     4141 tcctgctggg tatcagcagc gttctgctga cgctcctcat cctgctccac aagggccgcg
     4201 gcggcggtct gtccgacatg ttcggcggtg gtatgtccgc ggccgtcggc tcgtccggcc
     4261 tcgcggagcg gaacctcaac cggttcaccg tgatcctcgc gctggtctgg ttcgtctcca
     4321 tcgtcgcgct cggcctcatc acgaagttct cggtgatctg atggccaccg gaggaaacgc
     4381 gatccgcggc acgcgggtcg gatcgggccc catgggggag caggaccacg gctaccacgc
     4441 ggaccgcatc gcggtgtcgt actgggacgg cctgggcaac gagaccgtgc gctacttcgc
     4501 cgcgggcctg cccgaggagg agatccccga cacgatcgac cacccgcaca ccggtcttcc
     4561 ggccggtcgc gaccgtcaga acccgccggc tctggccaag gccgagccgt acaagacgca
     4621 cctcgcctac gtgaaggagc gtcgcacgga cgaagaggcc gagcagctgc tcgaggacgc
     4681 gctcacgcag ctccgcgagc gtcgcggcca gcgctgacgc gcaccacgca gttcgcacag
     4741 gacccccggg actcggttcc gggggtcctc tgcgttcgga gcgggtcccg ccgtccacgg
     4801 gatcgcgcgc tcagctgcag gtgctgccga ggccgaggag gatcacgttg agcgtgaagg
     4861 tcttgggagg cgcgtaccag gtcgtgttcg gatagccggt ctggatgctg atgccgtagg
     4921 ttccgccgag aaggtcgctg aggttcagca gcccgagcag cttcgcggtg tcgtacgtgg
     4981 cggcgtagga gtacacgccg cccgtctgac ccgtcagcgt gatctggctc gtgtcgcggg
     5041 cggtggtgga gttcttggtg atgtccacgc gctgatcagc cagcggcagc gtcgacgacc
     5101 aggcgaggtc gaccctctgc agcccgacca cgatggcgct cgtgggtttg caggtcgctc
     5161 cggtcgccgt ggtgggggcg gggaccgcgg tgacggtcgt gttcgtcacc gcgtcagccg
     5221 tccaggccgc ctccgtcgtg cggaggccgg gcagcagcac gcccgtcagg cagagaagcc
     5281 c
//
LOCUS       DATMSX010000567        20188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_75373,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000567 DATMSX010000000
VERSION     DATMSX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75373"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN041_18005"
     CDS             <1..445
                     /locus_tag="VN041_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWU30979.1"
                     /translation="ALFALAACLVLIVGGGIATAVVVSQLQRPASVVALDEIRSAPDA
                     AQATVKLGSGATATAHWSADLGEAVLVASGLERPEAGKTYELWFVRGDKPVSAGVFTP
                     EGGSATALLNGSMRAGDVIAVTVEQSGGSPSGAPTSEPIIVIPTA"
     gene            467..1096
                     /locus_tag="VN041_18010"
     CDS             467..1096
                     /locus_tag="VN041_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWU30980.1"
                     /translation="MPEQFHRPVRRPTAAFDNLVGAADPAEKSRIAHATASALLERAR
                     KDVTGETTERLIAFASEHGIDEIAELWSHAPARSLPGALWRLYLLQIAIRSDAATTAL
                     LYERGRVELQSADSLIAGAPTPASPEELAELVDTILRGVFQGDFAVALERAAAYCRVQ
                     ASGATHTADDYEPTEPGRASELTRRALRLSGYADDLSASAAAWRRGALT"
     gene            1276..2157
                     /locus_tag="VN041_18015"
     CDS             1276..2157
                     /locus_tag="VN041_18015"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HWU30981.1"
                     /translation="MAQRYALMIDPADVADTRDDYSDTFTDVEVEAPALSVGEFSTQR
                     GDGVFESIGVIDGHANEVEAHVQRLAHSAQICDLPAPNLEQWRQAVAAAAARIPQGEA
                     VVKLILSRGVEHGPAPTAWVTAAPAADFSAVRRDGVRVVTLDRGYDLGAGERAPWLLL
                     GAKTLSYAVNMAALREAHRRGADDAVFVTREGFVLEAPTASLIIRRGDEFLTPAPSGG
                     ILHGTTQLSVYELLESRGFRTTYATLSATDLATADAAWLVSSIRLAVAITAVDGAPLA
                     SDPDLTAELNAFLLSPR"
     gene            complement(2224..3003)
                     /gene="pstB"
                     /locus_tag="VN041_18020"
     CDS             complement(2224..3003)
                     /gene="pstB"
                     /locus_tag="VN041_18020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWU30982.1"
                     /translation="MSKSIEVNDLNVYYGDFLAVEGVSLEIQPRSVTAFIGPSGCGKS
                     TFLRTLNRMHEVIPGARVEGEVLLDGNDLYGPGVDPVLVRRQVGMVFQRPNPFPTMSI
                     KENVLAGVKLNNKRMSRSDEDALVEKSLIGANLWNEVKDRLDKPGSGLSGGQQQRLCI
                     ARAIAVSPEVILMDEPCSALDPISTFAIEELIQELKNDYTVVIVTHNMQQASRVSDKT
                     AFFNIAGTGKPGKLIEYDETATIFTTPSVQATEDYVSGRFG"
     gene            complement(3029..4120)
                     /gene="pstA"
                     /locus_tag="VN041_18025"
     CDS             complement(3029..4120)
                     /gene="pstA"
                     /locus_tag="VN041_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWU30983.1"
                     /translation="MTITENAPAAVQLAPSARITNGQLPAWSPWALLAASLVVSAAVF
                     GVVAAGGGGFNLVGTVIVGVLLYVVLIGVISSLVEGRRKATDRIVTALVASAFGIAMV
                     PLVSVAYTALQNGIAGLNAEFFSSSMRNVVGEGGGALHAIMGTVLITLAAAVISIPIG
                     LLTSIYLVEYGRGRLARGITFLVDVMTGIPSIVAGLFIYALFALILTPGIRMGLMGAL
                     SLAVLMIPVVVRSSEEMLRLVPNELREASYALGVPKWLTILKIVLPTSLAGIVTGVTL
                     SIARVIGETAPLLVTAGFTASMNYSLTDGRMMSLPVFVYTQYMNQGLPAEAYVNRAWA
                     AALVLIVIVMVLNLIARLVARYFAPSKSR"
     gene            complement(4122..5054)
                     /gene="pstC"
                     /locus_tag="VN041_18030"
     CDS             complement(4122..5054)
                     /gene="pstC"
                     /locus_tag="VN041_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWU30984.1"
                     /translation="MTTTATAVSAKQRPGDRWFSGAALSAGTMILVTLAAVAVFLIIQ
                     SIPGITATNEDASILKSDFWSYVGPLAFGTVWAAAIALLIAIPLSIAVALFISHYAPR
                     RLAQGLGYIVDLLAAVPSVVFGIWGIAVLAPAALPVYTWMTENLGWIPLFDGNASGRN
                     ILTAAIVLAVMVLPIITAICREIFLQTPPLHEEAALALGATRWEMIRMAVLPFARGGI
                     VSASMLGLGRALGETMAVAMVLSASGGITWRLLTAQNPNTIPANIAQTFPEAYGVNIN
                     VLIATGLILFVVTFLVNAVARWIVSRRKEFSGAN"
     gene            complement(5161..6270)
                     /gene="pstS"
                     /locus_tag="VN041_18035"
     CDS             complement(5161..6270)
                     /gene="pstS"
                     /locus_tag="VN041_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWU30985.1"
                     /translation="MKISRIARITAIGAVAALALAGCAANENSTPAGSEKPSSGPALS
                     GNLVGGGATSQEVAIAAWTKAFQTANGDVTVDYDPQGSGAGRDSFAAGAYNFAGSDRA
                     FKTDELSSMEFKGCEADSPIVEIPAYISPIAVIFNLDGVDKLNLDADTLAGIFAGTIT
                     KWNDEKIAATNPDVTLPDLAINPVHRADKSGTTGNFTDYLSAAAGSVWTHGSVEEWPI
                     QSGEAAQGTSGVVQAVEGGKGTIGYADASRAGKLGTVSVKVGDKFVPYSPEAAAAIVD
                     HSPLEERSGKADLAVKLDRTSTEAGVYPVVLVSYLIGCEKYADPAKAALVKGFFTEAI
                     SAEGQKAAAEAAGSAPISDTIRTKAQAAIDLIVTK"
     gene            complement(6394..7332)
                     /locus_tag="VN041_18040"
     CDS             complement(6394..7332)
                     /locus_tag="VN041_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWU30986.1"
                     /translation="MTDTAVFAAGAVVWRLVEDKLRILLIHRTKYRDVTLPKGKVDPG
                     EMLAQTAVREVREETGIKVTLGVPVGVSRYWMRPKRQKVVHYWAAEATDEAIRASSFV
                     PNGEISGVEWVSLKKARSRLSYPVDVEILDNFARLVDDGVLHTFPLIALRHAKAVGRD
                     DWDGADAERPLTDRGQRQAAAVVAPLRAFGVRRIVSSDALRCRQTVAPLEKKLSRTAR
                     VTAKISQDAWENGTEDIRAVVGRRVRARKAAVLCSHGPVLPGILSELALATGTMHGSY
                     VDSASSLGMAGFSVVHLSATNPGSGIISIETHEPKI"
     gene            complement(7329..9488)
                     /locus_tag="VN041_18045"
     CDS             complement(7329..9488)
                     /locus_tag="VN041_18045"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HWU30987.1"
                     /translation="MIEPVVVDAGLGDAEDDDFDAHEAPDAVLPDHRYHDRELSWLAF
                     NQRVLELAEDPALPELERANFLAIFASNLDEFFMVRVAGLKRRILTGLAVPTNVGRAP
                     TDVLSDISAEAHALQLRHADAWTEQVRPALADAGIEITEWGDLTDAERGALGEYFQAQ
                     VFPVLMPLAVDPAHPFPYISGLSLNLAIRIRNARTGRQDFARLKVPPMLPRFVEVPGA
                     GEILRFIRLEELIANHLDDLFPGMEVLDHHAFRLTRNEDVEIEEDESENLIQALEAEL
                     LRRRFGPPIRLEITDDMDDVTLELLIKELDITDQEVYRLPGPLDLRGLFDLARIDRPD
                     LRYPPHLPTTAVAFQPGDSNERPDIFKAIGRADVLVHHPYESFATSVQAFLEQAARDP
                     QVLAIKQTLYRTSGDSPIVQALIDAAEAGKQVLALVEVKARFDEANNIVWARKLEKAG
                     VHVVYGLVGLKTHCKLALVIREEDGVLRHYSHVGTGNYNPKTSRIYEDFGLFTADPQI
                     GKDLTRLFNELSGYAIEKKFKRLLVAPLHLRKGLLRLIDRERRNAEEGKAARVRIKVN
                     SMVDEQIIDALYRASMAGVKVEVWVRGICSLRADLPGVSDNITVRSILGRYLEHSRIF
                     AFDNDGDPIVYIGSADMMHRNLDRRVEALVRLSAPEHIQELHDLFDLAMDDGTNSWHL
                     EDGAWTRAVGDEDAPLIDLQDRTMVAVQSRRRKRTVR"
     gene            complement(9529..10209)
                     /locus_tag="VN041_18050"
     CDS             complement(9529..10209)
                     /locus_tag="VN041_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30988.1"
                     /translation="MALLLVLSTSADAESLLPALGLLSHGIRRHPLDTGHLLTLPDAD
                     AILVDARSDLVAARTACRFLRTATVELPVLLVVTEGGLSAVAADWGFDDVVLHDAGPA
                     EIDARLRLALSRAAADDEPTRIQASGITIDEQSYSARLHGRPLDLTYKEFQLLHFLAT
                     HPSRVFTREQLLSDVWGYDYFGGTRTVDVHVRRLRAKLGEAEQIIGTVRNVGYRFNLN
                     DEDHRAND"
     gene            10282..10575
                     /locus_tag="VN041_18055"
     CDS             10282..10575
                     /locus_tag="VN041_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30989.1"
                     /translation="MSEPALAPRNAFHGVVAVWIASLVTAIVLGVVLPEQVRVTWLLI
                     AFGAMVLVAFAVQLAYGRAQGFIVRVAGSVVGALVVMGLVSAVFGLTALAAAL"
     gene            10730..11338
                     /locus_tag="VN041_18060"
     CDS             10730..11338
                     /locus_tag="VN041_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HWU30990.1"
                     /translation="MIDLPTDLPADLAPLSWLLGVWEGTGVIEYTAGETRFQGEFSHR
                     VSFSHDGGPFLNYAATASFLGLEGQDEPVQLLAETGFWRLARPRTDADPGPALLPPAV
                     AGPERTVDDVEQLRSADGFPIEVSVAHSDGTLELYLGRIAGPRIDISTDAVVRPAGAA
                     TKEYTAASRMYGLVDGHLLWAWDITALGEQLKSHASARLAKV"
     gene            11341..12435
                     /locus_tag="VN041_18065"
     CDS             11341..12435
                     /locus_tag="VN041_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HWU30991.1"
                     /translation="MSAFDPLPGAVRDGDLITHFGNPIIEQRQLAHGEALAPLTDRVL
                     IEVAGEDRLSWLDSFTSQSLARLRPGASTELLILDPQGRVEHAAGVLEDGASVWLIAD
                     AGDAEQLATWLKRMVFRSRVTVTVQADAALVGFFAGGDAEGRITASAPHSVPLVWRDP
                     WREVQPGGHQYALVDGHPAADYAWSVAVVENASALTGPFAGALAAEALRVAAWRPRWS
                     HEVDERTIPHESDWIRSAVHLNKGCYRGQETVAKVHNLGHPPRRLAALHLDGSDVVLP
                     APGDVVLAGDDEVGHITSAVLHYEEGPIALAILSRRAPVGDLVVRSEGVDIAAAQQVI
                     VPADAGATADVPRLTRLSRRPVGEDPRNAR"
     gene            12634..13566
                     /locus_tag="VN041_18070"
     CDS             12634..13566
                     /locus_tag="VN041_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU30992.1"
                     /translation="MTGELKTRMLWSDLPDEVVREVERVLDAPVVEAFSQAEGFSPGS
                     ADRVVAADGRRAFVKTVHRDRNEGAYELHQREIQVMRLLPGTVSAPALLGSLVTDEWA
                     VLVLADVEGRHPGGEGDGSDVIAVLDAFSTFPRLEGESLSMLPSAVDEFVEEQDSWRV
                     IEAEDVELPAWAAANRGRLRAAAERVLEVVAGDHLQHFDGRADNVLMDAEGTAWIIDW
                     PWASVGARWMDGLFYLLDVRLRGEAVDVEEVLAQHPLFDGVAPADVDSALAAVTGHFF
                     VKAQLPAPPGMPTLRDFQYREALAGMEWLQQRWA"
     gene            complement(13568..14389)
                     /locus_tag="VN041_18075"
     CDS             complement(13568..14389)
                     /locus_tag="VN041_18075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWU30993.1"
                     /translation="MSGPVGQPTRGTTGTNRLRRNDRWIAASAAFRRAADPLVVDLGY
                     GASGVTAFELAQRLRQNRADAEVIGLELDPERVATARSQLRDVRAGRTSFASDLPVSF
                     ERGGFEVPLPGGRSPAVIRAMNVLRQYDEADVADAWRLVASRLAPDGMLFEGTCDEVG
                     RIASWVDVASDGEPVRFTISLRLADLERPGIVAERLPKALIHRNVPGERIHDLLTALD
                     REWDRAAPLSTFGPVQRWLAAVTALRAQGIPVQGTKSRWRLGELTVPWPTVAPAL"
     gene            14625..15368
                     /locus_tag="VN041_18080"
     CDS             14625..15368
                     /locus_tag="VN041_18080"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HWU30994.1"
                     /translation="MTRTLILLRHGQSEWNQLNLFTGWVDVRLTEQGKTEARRGGELL
                     AESGILPDVLHTSVLSRAIQTANIALDAADRLWIPVTRSWRLNERHYGALQGKDKAQT
                     LEEFGQEQFMLWRRSFDVPPPLLDDASEFSQVNDPRYVGIDGEVPRTESLKLVIDRLL
                     PYWESAIVPDLEAGKTVLVTAHGNSLRGLVKHLEGISDDDIAELNIPTGIPLVYELDD
                     DNVPTGPGRYLDPEAAAAGAAAVAAQGKK"
     gene            complement(15429..16145)
                     /gene="phoU"
                     /locus_tag="VN041_18085"
     CDS             complement(15429..16145)
                     /gene="phoU"
                     /locus_tag="VN041_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWU30995.1"
                     /translation="MREVFHQSLEELQSRLAEIADLVTVAIEKATTAFAESDVALAEE
                     VIADDAKIDELAVALDEQAIEILARQQPVARDLRIVITALRVSASLERMGDMAEHIAQ
                     LARLRFPERAIPKGLKGTFRRMGELDVEISRTLAELLRTQDLRFADAIRNADDDVDEL
                     HASVFEKVLSDNWKGEATATVDATLASRYHERFADHAVAVAKKVVYLATGDWAVDEQD
                     IALAAEAQSSASDAPEAPAN"
     gene            16331..17572
                     /locus_tag="VN041_18090"
     CDS             16331..17572
                     /locus_tag="VN041_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU30996.1"
                     /translation="MPSPQLALFALVIGIVIGAGVVLLVTWAYRMRMQAIQDTSQSIP
                     AGITEVLAGMDDAACVLDPSGLVVATSRAAARFDIRPGAVLENGELRRLVRDVRSSGI
                     SGTETLRITAGNVLDPRLVSARASVVGPRLTLLIVRDVTERERLDQMRTDFVSNTSHE
                     LKTPVASVSLLAEAIESAADDPDQVRIFASRIMAEAGRLGQLTGRIMSLSRLQAAESL
                     AQVEPVSIDEVVHASIEAHAVQADSAGVELTRGGDHGAWVRGDAQVLVEAVGNLLANA
                     IQYSPHGSRVGIGIRVDGDLVEIAVTDQGIGISEEDRERIFERFYRADDARSRRTGGT
                     GLGLSIVKHATQRHGGEVRVWSRAGKGSTFTMRLPRIDAPGADGAGASKKSKKKHKKK
                     TAKRARTDHGSVGAARNGENA"
     gene            17569..18252
                     /locus_tag="VN041_18095"
     CDS             17569..18252
                     /locus_tag="VN041_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU30997.1"
                     /translation="MTRILLVEDEPDLADPLAYLLRREGYEVEISEDGPGALEAFRAR
                     GADIILLDLMLPGMPGTEVCRQVRQASGVPIIMLTAKDSEVDIVVGLELGADDYITKP
                     YSSRELLARMRAVLRRVAQAETEIDERVLDGGRVTLDIDRHTVAVDGTEINMPLKEFE
                     LLEVLMRNAGRVLTRGQLIDRVWGSDYFGDTKTLDVHIKRIRSRIEQNPSDPVMLVTV
                     RGLGYRFEA"
     gene            complement(18320..18862)
                     /locus_tag="VN041_18100"
     CDS             complement(18320..18862)
                     /locus_tag="VN041_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HWU30998.1"
                     /translation="MNSRSAASLAPRLIAAAALGVTIALGATGCTFMTPQNTVDIKNI
                     SDGVDVSSTGGPVIVRNALIVADEDGSAGNLVAVLVNDTDESATLTIDVDGKQREVRV
                     PAGEHVSLGADEDPLLFENLDVKPGATVEVLFRSGDDDAEPTQVPVLDGTLPYLKDLV
                     PSPKPSVEPATPTPTGTPAS"
     gene            19017..19499
                     /locus_tag="VN041_18105"
     CDS             19017..19499
                     /locus_tag="VN041_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWU30999.1"
                     /translation="MLFEVGETVVYPHHGAATIIEVKDRVIKGETKKYLKLNVTQGDL
                     IIEVPAENVDLVGVRDVIGQEGLDHVFEVLRAPFTEEPTNWSRRYKANLEKLASGDVI
                     KVSEVVRDLWRRDQDRGLSAGEKRMLAKARQILVSELALAEKIDEDKAGGLLDEVLAS
                     "
     gene            19594..>20188
                     /locus_tag="VN041_18110"
     CDS             19594..>20188
                     /locus_tag="VN041_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWU31000.1"
                     /translation="MTPYSAPTTAIIIVAAGSGTRLGAGVPKALVDIDGRTVLRHALE
                     GVFAAQPMHVILVAPPGHEQAAADELEDVNSEHDWRATVVVGGETRQQSVAAGLAALG
                     DGIEVVLVHDAARALTPSAQIDAVAATVTADAGVIPALPVVDTLKRVEGDAVLEAVDR
                     SVLAAAQTPQGFPRGPLEAAYARALETGEDHTDDAALF"
BASE COUNT         3007 a         6764 c         7283 g         3134 t
ORIGIN      
        1 ggcgctcttc gcgctcgccg cgtgcctcgt gctgatcgtc ggcggtggca tcgccaccgc
       61 ggtggtcgtc tcccagctgc agcgccctgc ctccgtcgtc gcgctcgacg agatccgctc
      121 ggcgccggat gccgctcagg cgacggtgaa gctgggatcc ggagcgacgg ccaccgcgca
      181 ctggtcggcc gacctgggcg aggcagtgct cgtggcatcc gggctcgagc ggccggaggc
      241 cggcaagacc tacgagctct ggttcgtgcg cggtgacaag cccgtctccg cgggggtctt
      301 cacacccgag ggcggcagtg cgacggctct gctgaacggg tccatgcgcg ccggtgacgt
      361 gatcgccgtc acggtggagc agtccggcgg ctcgccctcc ggcgcgccga cgagcgagcc
      421 gatcatcgtg atccccacag cctgaggctg gcgtaccctg gatcgagtgc cagagcagtt
      481 ccaccgtccc gtccggcgtc cgaccgcggc cttcgacaac ctcgtcggag ctgcggaccc
      541 cgccgagaag tcgcgcatcg cgcacgccac ggcatccgct cttctcgagc gggctcgcaa
      601 ggacgtcacg ggggagacga ccgagcgact gatcgccttc gcgtccgagc acggcatcga
      661 cgagatcgcc gagctctggt cgcacgcgcc cgcgcgctcg ctgcccggtg cactgtggcg
      721 actgtacctg ctgcagatcg cgatccgcag cgatgccgcg accaccgccc tgctgtacga
      781 gcgcggcagg gtcgagctgc agtccgcgga cagcctgatc gcgggggcac cgacaccggc
      841 cagcccggag gagctcgccg agctcgtcga cacgatcctg cgcggggtgt tccagggcga
      901 cttcgccgtg gcgctcgagc gcgcggccgc ctactgccgg gtgcaggcgt ccggcgccac
      961 gcacaccgcc gacgactacg agcccaccga gcccggccgt gccagcgagc tcacccgccg
     1021 tgctctgcgc ctgagcggct atgccgacga cctgtccgcc agtgcggccg cctggcgtcg
     1081 cggcgcgctg acctgaggcg acggacgcgc tcgcctgaga cgtcgtccgg acagagaaag
     1141 accgggccgc aagaacgcct ctcggcggaa ggccgctcgc agcggcagaa gttggagccc
     1201 ggggttatgc ggcccggccg atcaagtgta acgcgtcttc ggcggaggta ttcccgaccg
     1261 tcctaagctg tggacatggc tcagcgctac gccctgatga tcgaccccgc cgacgtcgcg
     1321 gatacccgtg acgactactc cgacaccttc acggacgtgg aggtcgaagc acccgcgctc
     1381 agcgtggggg agttcagcac ccagcgcgga gacggcgtct tcgagtcgat cggcgtgatc
     1441 gacggacatg cgaacgaggt cgaggcgcac gtgcagcggc tggcgcattc cgcgcagatc
     1501 tgcgacctgc cggcaccgaa cctcgagcag tggcggcagg ccgtcgcggc cgccgccgca
     1561 cggattccgc agggcgaggc cgtcgtcaag ctgatcctga gccggggcgt ggagcacgga
     1621 cctgcgccga ccgcgtgggt caccgccgcg cccgccgccg acttctccgc agtccgccgc
     1681 gacggcgtgc gcgtcgtcac tctcgaccgc ggctacgacc tcggagcggg ggagcgggcg
     1741 ccgtggctgc tgctcggcgc gaagaccctc tcctacgcgg tgaacatggc cgcgctccgc
     1801 gaggcgcacc ggcgcggagc cgacgacgcc gtgttcgtca cccgcgaggg gttcgtgctc
     1861 gaggcaccca cggcatcgct gatcatccgc cgcggcgacg agttcctcac gcctgcgccg
     1921 tccggcggca tcctgcacgg cacgacgcag ctcagcgtgt acgagctgct ggagtcgcgc
     1981 ggcttccgca cgacgtacgc gacgctctcg gccaccgacc tggcgaccgc ggatgccgcc
     2041 tggctggtct ccagcatccg cctggccgtg gcgatcaccg ccgtggacgg cgcaccactg
     2101 gcatccgatc ccgacctcac ggcagagctg aacgccttcc tgctgtctcc gcgctgaggc
     2161 ggacgccgca gcggcccctg atcgttcacc gatcaggggc cgctgccgtt cagacgccga
     2221 agatcacccg aagcgaccgg agacgtagtc ctcggtggcc tgcacggacg gggtggtgaa
     2281 gatcgtggcg gtctcgtcgt actcgatgag cttgccgggc ttgccggtgc cggcgatgtt
     2341 gaagaacgcc gtcttgtcgc tcacccgcga ggcctgctgc atgttgtgcg tgacgatcac
     2401 gaccgtgtag tcgttcttga gctcctggat gagctcctcg atcgcgaagg tcgagatcgg
     2461 gtcgagcgcc gagcagggct cgtccatcag gatgacctcg ggggagaccg cgatcgcacg
     2521 ggcgatgcac agacgctgct gctggccgcc cgacagccca gagccgggct tgtcgaggcg
     2581 gtccttgacc tcgttccaga ggttcgcgcc gatcagcgac ttctcgacca gcgcgtcctc
     2641 gtccgagcgc gacatgcgct tgttgttcag cttgacgccc gcgagcacgt tctccttgat
     2701 cgacatcgtc ggaaacgggt tcgggcgctg gaagaccatg cccacctggc ggcggaccag
     2761 caccgggtcg acaccggggc cgtacaggtc gttgccgtcc agcagcacct caccctcgac
     2821 gcgggcgccg gggatgacct catgcatacg gttcagggtg cgcaggaacg tggacttgcc
     2881 gcagccggaa gggccgatga acgcggtgac gctgcggggc tggatctcga gggagacgcc
     2941 ctcgacggcg aggaagtcgc cgtagtagac gttgaggtcg ttgacttcga tgctcttgga
     3001 cacgatgttc cttcgggttg gatcaggctc agcggctctt ggacggagcg aagtagcggg
     3061 cgacgagtcg cgcgatgagg ttgaggacca tgacgatgac gatcagcacc agggcggccg
     3121 cccaggcgcg gttcacgtat gcctcggcgg gcaggccctg gttcatgtac tgggtgtaga
     3181 cgaagaccgg cagcgacatc atccggccgt cggtcaggct gtagttcatg gatgccgtga
     3241 atccggcggt gaccagcagc ggcgcggtct cgccgatcac gcgtgcgatc gagagcgtca
     3301 cgccggtcac gatgcccgcc agggacgtgg gcagcacgat cttgaggatc gtgagccact
     3361 tcggcacgcc cagcgcgtag gaggcctcgc gcagctcgtt gggcaccagt cgcagcatct
     3421 cctcgctgga gcgcacgacg accgggatca tcaggacggc cagcgacagc gcgcccatca
     3481 ggcccatccg gatgccgggg gtgaggatca gcgcgaagag ggcgtagatg aacagacccg
     3541 cgacgatcga cgggatgccc gtcatcacgt cgacgaggaa cgtgatgccg cgcgcaagcc
     3601 gaccgcggcc gtactcgacc aggtagatcg aggtgagcag gccgatcggg atggagatca
     3661 cggcggccgc cagggtgatc agcacggtgc ccatgatggc gtgcagtgcg ccgccgccct
     3721 cgcccacgac gttgcgcatg gagctggaga agaactcggc gttcaggccg gcgatgccgt
     3781 tctgcagcgc ggtgtaggcg accgacacga gcgggaccat ggcgatgccg aaggccgagg
     3841 cgaccagcgc cgtcacgatg cgatcggtgg ccttgcggcg gccctcgacg agcgaggaga
     3901 tcacgccgat caggaccacg tacagcagga cgccgacgat gaccgtcccg acgaggttga
     3961 atccgccgcc gccggctgcg accacgccga acacggcggc ggagacgacc agggatgcgg
     4021 ccaggagcgc ccacggggac caggcgggca gctgcccgtt ggtgatgcgg gcggacggtg
     4081 cgagctgcac cgcggcgggg gcgttctcgg tgatggtcat gtcagttcgc tcccgagaat
     4141 tccttgcggc ggctcacgat ccagcgcgcg acggcgttga ccaggaacgt gaccacgaag
     4201 aggatcaggc ccgtggcgat caggacgttg atgttcacgc cgtacgcttc ggggaaggtc
     4261 tgtgcgatgt tcgcggggat ggtgttgggg ttctgtgcgg tgagcaggcg ccaggtgatg
     4321 ccgccggacg ccgagagcac catggcgacg gccatggtct cgccgagcgc gcggccgagg
     4381 cccagcatcg atgcggacac gatgccgccg cgggcgaagg ggagcaccgc catgcggatc
     4441 atctcccagc gcgtggcgcc cagcgcgagc gcggcctcct cgtgcagcgg cggggtctgc
     4501 aggaagatct cgcggcagat ggcggtgatg atcggcagca ccatgacggc cagcacgatc
     4561 gccgcggtca ggatgttgcg tccggaggcg ttgccgtcga acagcgggat ccagcccagg
     4621 ttctcggtca tccaggtgta gaccgggagg gcggctgggg cgagcacggc gatgccccag
     4681 atgccgaaga cgacggaggg cacggcggcc agcaggtcga cgatgtaacc gagcccctgt
     4741 gcgagcctgc gcggtgcgta gtgcgagatg aacagcgcca cggcgatgga gagcgggatc
     4801 gcgatcagca gggcgatcgc cgctgcccag accgtgccga aggcgagcgg gccgacgtag
     4861 gaccagaagt cgctcttcag gatcgaggcg tcctcgttgg tcgccgtgat gccggggatc
     4921 gactggatga tcaggaacac cgcgacggcg gcgagcgtca cgaggatcat cgtgccggcg
     4981 gacagcgcgg ctccggagaa ccagcggtcg cctggacgct gtttcgccga caccgcggtg
     5041 gcagtggtgg tcatctcggg atgtcctcgc tcgtctgagt ggtgttttcc gtgcggatgc
     5101 gggatgtgga gcagacggcg ggccgacgac gctgtgcgtc gtcgacccgc cgtccgaagg
     5161 tcacttggtg acgatcaggt cgatcgcggc ctgtgccttg gtacggatcg tgtccgagat
     5221 cggggcggag cctgcggcct cggccgcggc cttctggccc tcggcgctga tcgcctcggt
     5281 gaagaagccc ttcaccagtg cggccttggc cgggtcggcg tacttctcgc agccgatcag
     5341 gtagctgacc aggacgacag ggtagacgcc ggcctcggtg ctggtgcggt cgagcttgac
     5401 ggccaggtcg gccttgcccg agcgctcctc cagcggcgag tggtcgacga tggcggcagc
     5461 agcctcgggg gagtacggca cgaacttgtc gccgaccttc accgagacgg tgccgagctt
     5521 gccggcgcgc gaggcgtcgg cgtagccgat ggtgcccttg ccgccctcga cggcctggac
     5581 gacgcccgag gtgccctgtg cggcctcgcc ggactggatc ggccactcct cgaccgagcc
     5641 gtgggtccag acggagcctg cggcggccga caggtagtcg gtgaagttgc ccgtggtgcc
     5701 ggacttgtcc gcgcggtgca ccgggttgat ggccaggtcg ggcagcgtga cgtcggggtt
     5761 ggtggcggcg atcttctcgt cgttccactt cgtgatggtg ccggcgaaga tgccggcgag
     5821 ggtgtcggcg tccaggttca gcttgtcgac gccgtcgagg ttgaagatca cggcgatcgg
     5881 cgagatgtac gccgggatct cgacgatcgg ggagtcggcc tcgcagccct tgaactccat
     5941 ggaggagagc tcgtcggtct tgaacgcgcg gtcggagccg gcgaagttgt aggcgccggc
     6001 ggcgaaggag tcgcggcccg cgccggagcc ctgcgggtcg tagtcgacgg tgacgtcgcc
     6061 gttggcggtc tggaaagcct tggtccaggc tgcgatcgcg acctcctggg aggtggcgcc
     6121 gccgccgacg aggttgccgc tcagcgccgg gccggaggac ggcttctcgg agccggcggg
     6181 ggtgctgttc tcgttcgcgg cacagccggc gagggcgagg gcggcgaccg cgccgatggc
     6241 ggtgatgcgg gcgatacggg agatcttcac tgtggatccg ttcgatcggg agatgtgtgc
     6301 ggcccggtgc agggcacaca gtgacgttag gaggaccggt taacgagact ctccccgtcg
     6361 ggtgaacgga aggtgaacgg atcgcggcgg gagtcagatc ttgggttcgt gcgtctcgat
     6421 cgagatgatc ccggagccgg ggttggtggc ggagaggtgc acgaccgaga agcccgccat
     6481 ccccaggctg ctggcgctgt cgacgtagga gccgtgcatg gtcccggtcg ccagggccag
     6541 ctcggagagg atgccgggaa gcaccgggcc gtggctgcag agcacggccg ccttgcgggc
     6601 ccggacccga cggcccacga ccgcccggat gtcctcggtg ccgttctccc aggcatcctg
     6661 gctgatcttc gcggtgaccc gcgccgtgcg gctgagcttc ttctccagcg gtgccaccgt
     6721 ctggcggcag cgcagcgcat cgctggagac gatgcgccgc acgccgaagg cgcggagcgg
     6781 ggcgacgacg gcagccgcct ggcgctggcc gcgatcggtg agcgggcgct cggcatccgc
     6841 tccgtcccag tcgtcgcgcc ccacagcctt cgcgtgccgc agtgcgatca gcgggaaggt
     6901 gtgcagcacg ccgtcgtcca cgagccgtgc gaagttgtcg aggatctcca cgtcgaccgg
     6961 gtagctgagg cgcgaacgcg ccttcttcag gctcacccac tcgaccccgg agatctcgcc
     7021 gttgggcacg aaactggagg cccggatggc ctcgtccgtc gcctccgccg cccagtagtg
     7081 cacgaccttc tggcgcttcg gccgcatcca gtaccggctg acgccgaccg gcacgccgag
     7141 ggtgaccttg atgcccgtct cctcgcgcac ctcgcgcacg gcagtctgag ccagcatctc
     7201 gccggggtcg accttgccct tgggcagcgt gacgtcacgg tacttcgtgc ggtggatcag
     7261 caggatgcgc agcttgtcct cgaccaggcg ccacaccacc gcacccgccg cgaagaccgc
     7321 ggtgtccgtc atcggaccgt tcgcttgcgg cggcggctct gcacggcgac catcgtgcgg
     7381 tcctgcaggt cgatgagcgg ggcgtcctcg tccccgaccg cgcgggtcca ggcgccgtcc
     7441 tccaggtgcc aggagttcgt gccgtcgtcc atcgccagat cgaacaggtc gtgcagctcc
     7501 tggatgtgct cgggagccga cagcctgacc agcgcctcga cgcggcgatc gaggttgcgg
     7561 tgcatcatgt cggcgctgcc gatgtacacg atcgggtcgc cgtcgttgtc gaaggcgaag
     7621 atgcgggagt gctcgaggta gcggcccagg atgctgcgca ccgtgatgtt gtcgctgaca
     7681 ccgggcaggt ccgcccgcag gctgcagatg ccgcgcaccc acacctcgac cttcacaccc
     7741 gccatgctcg cgcggtacag agcgtcgatg atctgctcgt cgaccatcga gttgaccttg
     7801 atgcgcacgc gcgccgcctt gccctcctcg gcgttgcggc gctcgcggtc gatgagccgc
     7861 agcaggccct tgcgcaggtg caggggcgcg acgagcaggc gcttgaactt cttctcgatc
     7921 gcgtagccgc tcagctcgtt gaagagcctc gtgaggtcct tgccgatctg cggatcggcg
     7981 gtgaacagcc cgaagtcctc gtagatgcgg ctggtcttcg ggttgtagtt gcccgtgccg
     8041 acgtgcgagt agtggcgcag cacgccgtcc tcctcgcgga tcaccagtgc cagcttgcag
     8101 tgcgtcttca ggcccaccag gccgtacacg acgtgcacgc cggccttctc gagcttgcgc
     8161 gcccagacga tgttgttcgc ctcgtcgaag cgcgccttca cctccaccag cgccagcacc
     8221 tgcttgccgg cctctgcggc gtcgatcagc gcctgcacga tcgggctgtc gcccgaggtg
     8281 cggtacaggg tctgcttgat ggccagcacc tgcgggtcgc gggccgcctg ctccaggaac
     8341 gcctgcacgc tggtcgcgaa cgactcgtac ggatggtgca ccagcacgtc ggcgcggccg
     8401 atcgccttga agatgtcagg gcgctcgttg ctgtcgccgg gctggaacgc gaccgccgtg
     8461 gtgggcaggt ggggcgggta gcgcaggtcg ggccggtcga tgcgggccag atcgaacagg
     8521 ccgcgcagat cgagcgggcc ggggaggcgg tagacctcct ggtcggtgat gtcgagctcc
     8581 ttgatcagca gctcgagcgt cacgtcgtcc atgtcgtcgg tgatctcgag gcggatcggc
     8641 gggccgaagc gacggcgcag cagctcggcc tcgagagcct ggatgaggtt ctcggactcg
     8701 tcctcctcga tctccacgtc ctcgttcctg gtgaggcgga acgcgtggtg atcgagcacc
     8761 tccatgccgg ggaagagatc gtccaggtgg ttggcgatca gctcctccag gcggatgaac
     8821 cggaggatct cgccggcgcc cggcacctcc acgaaccggg ggagcatcgg cggcaccttc
     8881 aggcgcgcga agtcctgacg tccggttcgg gcgttgcgga tacggatggc gaggttcagc
     8941 gacagtccgg agatgtaggg gaacgggtgc gccgggtcga cggccagcgg catgagcacc
     9001 gggaacacct gggcctggaa gtactctccc agcgcgcccc gctcggcatc cgtcagatcc
     9061 ccccactcgg tgatctcgat accggcatcc gccagtgccg gcctgacctg ttcggtccac
     9121 gcatcggcgt ggcgcagctg cagggcgtgc gcctcggcgg agatgtcgga gagcacatcg
     9181 gtcggcgcgc ggccgacgtt ggtcggcacc gcgagccccg tgaggatgcg gcgcttgagc
     9241 cccgcgacgc gcaccatgaa gaactcgtcg aggttgctgg cgaagatcgc caggaagttg
     9301 gcccgctcca gctccggcag cgccgggtcc tcagccagct ccagcacgcg ctgattgaac
     9361 gccagccagc tcagttcgcg gtcgtggtag cgatgatcgg gcagcacggc gtcgggcgcc
     9421 tcgtgcgcat cgaaatcgtc gtcctcggca tcgcccaggc cggcatccac aactacgggt
     9481 tcgatcatgg ggtacatcct cgcatccgcc ggtgacgtgt gggtgaactc agtcgttcgc
     9541 ccgatgatcc tcgtcgttca ggttgaaccg gtaccccacg ttgcgcaccg tgccgatgat
     9601 ctgctcggcc tcgccgagct tggcccgcag ccgtcgcacg tgcacgtcga ccgtgcgcgt
     9661 gcccccgaag tagtcgtacc cccacacgtc gctgagcagc tgctcgcgcg tgaacacgcg
     9721 ggacggatgg gtggcgagga agtgcaggag ctggaactcc ttgtaggtga gatccagcgg
     9781 gcggccgtgc agccgggcgg agtacgactg ctcgtcgatc gtgatgccgg atgcttggat
     9841 ccgggtgggt tcatcgtccg cggcggcgcg ggagagggcg agacgcagcc gtgcgtcgat
     9901 ctcggcggga ccggcgtcgt gcagcacgac atcgtcgaag ccccagtcgg cggcgacggc
     9961 gctgaggccg ccctcggtca cgacgagcag cacgggcagt tccacggtgg cggtgcgcag
    10021 gaaccggcag gcggtgcggg ccgcaacaag gtccgacctg gcgtccacga ggatcgcgtc
    10081 ggcgtcgggc agcgtgagca ggtggcccgt gtcgagcggg tgccggcgga tgccgtgaga
    10141 gagcagtccg agcgcgggca gaagcgactc cgcgtcggcg gaggtgctga gcacgagcag
    10201 cagggccacg cgttgagcat acttgccgtc gcggcgcggg tccgcagtaa tgtcacagcc
    10261 gtggatgagt acgatggccg catgtccgaa ccggctctcg ccccgcgcaa cgccttccac
    10321 ggcgtcgtcg ccgtgtggat cgcgtcgctg gtgacggcga tcgtcctggg cgtcgtgctt
    10381 cccgagcagg tccgtgtgac ctggctcctg atcgcgttcg gcgccatggt gctggtggcg
    10441 ttcgccgtgc agctcgccta cgggcgcgca cagggattca tcgtgcgggt cgcgggcagc
    10501 gtcgtgggcg cgctcgtcgt gatggggctg gtgtcggccg tgttcggcct gaccgcgctc
    10561 gccgccgccc tctgactccg caggtcgact agagtgatgc acatggatcc cgtcgcgctc
    10621 gaattcttct tcctcggtct gctgggactg gcgagccttg cgatcgcctt cgtcgccggc
    10681 gtcgtgctcg tcaacctgtt ccgcggccag cgctgatcag acgtccgtcg tgatcgatct
    10741 gcccacggat cttcccgccg acctcgcgcc gctctcgtgg ctgctgggcg tctgggaggg
    10801 caccggtgtc atcgagtaca ccgcgggcga gacgcgcttc cagggcgagt tctcgcaccg
    10861 cgtgagtttc agccatgacg gcggtccgtt cctgaattac gcggcgacgg cctccttcct
    10921 cggtctcgag ggccaggacg agcccgtgca actgctcgcc gagaccggct tctggcgtct
    10981 cgcgcggccg cgcacggatg ccgatcccgg cccggctctg ctgcccccgg ccgtcgcggg
    11041 cccggagcgc accgtcgacg acgtcgagca gctgcgcagc gccgacgggt tcccgatcga
    11101 ggtgtcggtc gcgcactccg acggcacgct cgagctgtac ctgggccgta tcgccggtcc
    11161 ccggatcgac atctccacgg atgccgtggt gcgccccgcg ggggctgcga ccaaggagta
    11221 cacggcggcg agccgcatgt acggcctggt cgacggtcac ctgctgtggg cctgggacat
    11281 caccgccctc ggcgagcagc tgaagtcgca cgcgtccgcg cgtctcgcga aggtctgagc
    11341 atgtccgctt tcgacccgct ccccggcgct gtgcgcgacg gcgacctgat cacgcacttc
    11401 ggcaacccga tcatcgagca gcggcagctc gcgcacggcg aggcgctcgc accgctgacc
    11461 gatcgtgtgc tgatcgaggt ggcaggggag gatcggctca gctggctgga ctcgttcaca
    11521 tcccagtcac tggcgcgact ccgtcccggc gcgagcacgg agctgctgat cctggatccg
    11581 cagggtcgcg tcgagcacgc ggccggcgtg ctcgaggacg gcgcctcggt atggctgatc
    11641 gcggatgccg gagacgccga gcagcttgcg acgtggctga agcgcatggt gttccgttcc
    11701 cgtgtcacgg tcacggtgca ggccgatgcc gccctggtcg gcttcttcgc cggaggcgac
    11761 gccgagggcc ggatcacggc atccgctccg cacagcgtcc ccctggtctg gcgtgatccg
    11821 tggcgcgagg tgcagcccgg aggccaccag tacgcgctcg tggacgggca tccggccgcc
    11881 gactacgcct ggagcgtggc cgtggtcgag aatgcatccg ctctgaccgg gcccttcgct
    11941 ggtgcgctcg ccgccgaggc gctgcgcgtc gccgcctggc gcccacgctg gtcgcacgag
    12001 gtcgacgagc gcacgatccc gcacgagtcc gactggatcc gcagcgccgt gcacctgaac
    12061 aagggctgct accgcgggca ggagacggtc gccaaggtgc acaacctcgg tcatccgccg
    12121 cgtcgcctgg ccgccctgca cctggacggc agcgacgtcg tgctgcccgc gcccggcgac
    12181 gtcgtgctcg caggcgatga cgaggtcgga cacatcacct cggccgtgct gcactacgag
    12241 gagggcccga tcgcgctggc gatcctgtcc cgccgggcac ccgtcggcga tctggtggtg
    12301 cgttccgagg gcgtcgacat cgcggccgcg cagcaggtca tcgtgccggc ggacgccggt
    12361 gcgactgcgg acgtgccccg tctgacgcgt ctctcccgcc gcccggtcgg cgaggacccc
    12421 cgcaacgccc gctgagtcct cggtccggcg gctcgcggtg ctgttccgcc gagtccgtga
    12481 gcccgtcgca agagtgttgc ggtcatccgc tgcccgcccg gcggtgtgag gggaggaaaa
    12541 ggtcggtgaa acgtcgcctt ggccgacgtt ttcctccttt cctgggcagg tcgatgtgct
    12601 ttcgcacccg aagatcccgg atagggtcga cggatgacgg gcgagttgaa gacgcggatg
    12661 ctgtggagcg acctccccga cgaggtcgtg cgcgaggtcg agagggtgct ggacgcgccg
    12721 gtggtcgagg ctttcagcca ggccgaggga ttctcgccgg gaagcgcgga ccgcgtcgtc
    12781 gccgcggacg ggcgccgggc gttcgtgaag accgtgcatc gcgaccgcaa cgagggcgcg
    12841 tacgaactgc accagcggga gatccaggtc atgcgcctgc tgcccggaac ggtgagcgcg
    12901 ccggctctgc tcggttcgct ggtcacagac gagtgggcgg tcctcgtgct cgccgacgtc
    12961 gaggggcgcc atcccggcgg ggagggggac gggtcggacg tgatcgccgt gctggacgcg
    13021 ttctcgacgt tcccgagact cgagggcgag tcgctctcga tgctgccgtc ggcggtcgat
    13081 gagttcgtcg aggagcagga cagctggcgg gtgatcgagg cggaggacgt ggagctgccg
    13141 gcctgggccg ccgccaaccg cgggcgactc cgcgcggcgg cggagcgtgt gctcgaggtc
    13201 gtggcaggcg atcatctcca gcacttcgac ggtcgtgcag acaacgtgct gatggatgcc
    13261 gagggcacgg cctggatcat cgactggccg tgggcgtcgg tcggtgcgcg ttggatggac
    13321 gggctgttct acctgctcga cgtgagactg cgcggagagg ccgtcgacgt cgaggaggtg
    13381 ctggcgcagc atccgctgtt cgacggcgtt gcgccggccg acgtggactc cgcgttggcg
    13441 gccgtcacag ggcacttctt cgtgaaggct cagctgcccg ccccgcccgg catgccgacg
    13501 ctgcgcgact tccagtaccg cgaggccctc gccgggatgg aatggcttca gcagcgctgg
    13561 gcctgagtca gagggcaggg gcgacggtgg gccacgggac ggtcagctcg ccgaggcgcc
    13621 agcgcgactt cgtgccctgc accgggatgc cctgagcccg aagcgccgtc acggcggcga
    13681 gccatctctg cacgggtccg aaggtcgaca ggggagcggc gcggtcccac tcccggtcga
    13741 gcgcggtgag caggtcatgg atccgctcgc cggggacgtt gcgatggatg agcgccttcg
    13801 gcaggcgttc ggcgacgatc ccggggcgct cgagatccgc gagccgcagc gagatcgtga
    13861 atcgcacggg ctcaccgtcg gatgccacgt cgacccagct cgcgatgcgc cccacctcgt
    13921 cacaggtccc ctcgaacagc atgccgtccg gggcgaggcg ggaggcgaca agccgccacg
    13981 catccgcgac gtccgcctcg tcgtactggc gcagcacgtt catcgcgcgg atcacggccg
    14041 ggctccgtcc gccgggcagc ggcacctcga agccgccccg ctcgaaggac accggcaggt
    14101 cggaggcgaa ggaggtccga cccgcgcgca cgtcccgcag ctgcgagcgg gccgtcgcca
    14161 cgcgctccgg gtcgagttcg aggccgatga cctcggcatc cgctctgttc tgccgcagtc
    14221 gctgggcgag ctcgaacgcc gtcacgccgc tggcgccgta acccaggtct acgaccagcg
    14281 gatccgccgc cctccggaac gcagcggatg ccgcgatcca gcggtcgttc cgccgcagcc
    14341 ggttcgtccc cgtcgtcccc cgtgtgggct gtccgacggg tccgctcata ccctcattct
    14401 cccttacccc accacacccc ccatccaggt cgctcggccc cctcctaacg ccagggttca
    14461 cgctcgtgca cgtgagggcg cagcgacgtt cccggagcga gcatcgggga gggacccgcg
    14521 cagcgggagg ggcacccgct ctgcgagcga agggaacgtc gctgcgcccg ccatccaagg
    14581 tcacggaccc catcccacgg catccgaaaa gatagtctgg acgcatgacc cgcactctga
    14641 ttcttctgcg tcacggccag agcgaatgga accagctcaa cctcttcaca ggctgggtcg
    14701 atgtccgcct caccgagcag ggcaagaccg aggcacgccg cggcggcgaa ctgctcgcgg
    14761 agtccggcat cctgcccgac gtgctgcaca cttcggtgct gagccgcgcg atccagaccg
    14821 cgaacatcgc tctggatgcc gccgaccggc tgtggatccc ggtgacccgc tcgtggcgcc
    14881 tgaacgagcg tcactacggc gccctgcagg gcaaggacaa ggcccagacg ctggaggagt
    14941 tcggtcagga gcagttcatg ctgtggcgcc gctcgttcga cgtgccgccg cccctgctcg
    15001 acgatgccag cgagttcagc caggtgaacg atccgcgtta cgtcggcatc gacggcgagg
    15061 tcccgcgcac cgagtcgctc aagctcgtca tcgaccgcct gctgccgtac tgggagtccg
    15121 cgatcgtccc cgatctcgag gcgggcaaga ccgtgctggt caccgcccac ggcaactcgc
    15181 tgcgcggtct ggtcaagcat ctcgaaggca tcagcgacga cgacatcgcc gagctgaaca
    15241 tccccaccgg catcccgctg gtgtacgagc tcgacgacga caacgtcccg accggccccg
    15301 gccgctacct cgacccggag gccgcggccg ccggtgccgc tgcggtcgcc gcgcagggca
    15361 agaagtaacg cgacagacga ggaagggcgg atgctgatgc agcatccgcc cttctttcga
    15421 ctcgacggtc agttcgccgg ggcctccgga gcgtcggatg ccgaggactg cgcctcggcg
    15481 gccagggcga tgtcctgctc gtccacggcc cagtcgccgg tggcgaggta cacgaccttc
    15541 ttcgcgaccg cgacggcgtg gtcggcgaag cgctcgtggt agcggctggc gagcgtggcg
    15601 tcgacggtgg cggtcgcctc gcccttccag ttgtcgctga gcaccttctc gaacacgctg
    15661 gcgtgcagct cgtccacgtc gtcgtcggcg ttgcggatcg catccgcgaa gcgcaggtcc
    15721 tgcgtgcgga gcagttccgc gagggtgcgg gagatctcga cgtccagctc gcccatgcgg
    15781 cggaaggtgc ccttcaggcc cttcgggatc gcgcgctccg ggaagcgcag ccgggcgagt
    15841 tgcgcgatgt gctcggccat gtcgcccatg cgctccagcg atgcgctgac gcgcagggcc
    15901 gtgatgacga tgcgcaggtc gcgggcgacc ggctgctggc gggcgaggat ctcgatggcc
    15961 tgctcgtcga gagcgacggc gagctcatcg atcttcgcgt cgtcggcgat gacctcctcg
    16021 gccagcgcga cgtcgctctc ggcgaaggcg gtggtggcct tctcgatggc caccgtgacg
    16081 agatcggcga tctcggccag ccgggactgg agctcctcga gggactggtg gaagacttcg
    16141 cgcatgtcgg cgcaaccttt cgtcgtgggg tcggcacgga tgccgagcgg gcacgcgcac
    16201 acgcgaaccg ggtcgatcat ccccatcgaa ggttaacgaa tggtgtcgtg atcgcgaaca
    16261 ctgcctcgcc cggcccgtga cggcggtctg aacggcggaa ggacggggat gctgccccta
    16321 ctctggaacc atgccctcgc cccagctcgc gctgttcgcg ctcgtcatcg gcatcgtcat
    16381 cggcgccggt gtcgtgctgc tggtgacctg ggcgtaccgc atgcggatgc aggcgatcca
    16441 ggacacgtcg cagtcgatcc cggccgggat caccgaggtg ctcgcgggaa tggacgacgc
    16501 cgcgtgcgtg ctggatccgt ccggactggt cgtcgcgacc tcccgcgcgg cggcgcgctt
    16561 cgacatcagg cctggagcgg tgctggagaa cggcgagctg cgacggctgg tgcgggatgt
    16621 gcgcagctcg ggcatcagtg gcacggagac gctgcggatc accgccggga acgtgctgga
    16681 tccgcgcctg gtgtccgcgc gggcgagcgt ggtgggcccg cgtctgacgc tgctgatcgt
    16741 gcgcgacgtc acggagcggg agcgcctgga ccagatgcgc accgacttcg tctccaacac
    16801 cagtcatgag ctgaagacgc cggtcgcgtc ggtcagcctt ctcgccgagg ccatcgaatc
    16861 cgccgccgac gaccccgatc aggtgcgcat cttcgcgtcg cgcatcatgg ccgaggcggg
    16921 tcgtctgggc cagctcaccg gacggatcat gagcctgtcg cggctgcagg cggccgagag
    16981 cctcgcccag gtggagcccg tctccatcga tgaggtcgtg cacgcctcga tcgaggcgca
    17041 cgcggtgcag gccgactccg ccggggtcga gctgacgcgc ggcggcgacc acggcgcctg
    17101 ggtgcgcggc gacgcgcagg tgctcgtcga agccgtcggg aacctgctcg ccaacgccat
    17161 ccagtactcg ccgcacgggt cgcgcgtcgg gatcggcatc cgggtcgacg gcgacctcgt
    17221 cgagatcgcc gtgaccgacc agggcatcgg catctcggaa gaggatcggg agcgcatctt
    17281 cgagcgcttc taccgcgccg acgacgcgcg ctcccgccgg acgggcggaa cggggctcgg
    17341 cctgtcgatc gtcaagcacg ccacgcagcg ccacggcggc gaggtgcgcg tctggtcgcg
    17401 cgcgggtaag gggtcgacct tcaccatgcg gctgccgcgc atcgatgcgc cgggagccga
    17461 cggggcaggc gcctcgaaga agtccaagaa gaagcacaag aagaagaccg ccaagcgcgc
    17521 ccgcaccgat cacggttcgg tcggcgccgc ccggaacgga gagaacgcat gacccggatc
    17581 ctgctcgtcg aggatgagcc cgacctcgcc gaccccctcg cctacctgct gcgccgcgag
    17641 ggctacgagg tggagatcag cgaggacgga cccggcgcgc tggaggcgtt ccgcgcacgc
    17701 ggcgccgaca tcatcctgct cgacctgatg ctccccggca tgccgggcac cgaggtgtgc
    17761 cggcaggtgc gccaggcctc gggcgtgccg atcatcatgc tcactgcgaa ggactccgag
    17821 gtcgacatcg tggtggggct cgagctcggc gccgacgact acatcaccaa gccctactcg
    17881 tcgcgcgagc tgctggcgcg catgcgcgcg gtgctgcgcc gggtcgcgca ggcggagacg
    17941 gagatcgacg agcgcgtgct ggacggcggc cgcgtcacgc tggacatcga ccggcacacg
    18001 gtcgccgtcg acggcaccga gatcaacatg ccgctgaagg agttcgagct gctcgaggtg
    18061 ctgatgcgca acgcgggccg cgtgctcacc cggggtcagc tgatcgaccg ggtgtggggg
    18121 agcgactact tcggcgacac caagacgctg gacgtgcaca tcaagcgcat ccgctcccgc
    18181 atcgagcaga acccgagcga cccggtgatg ctggtcaccg tgcgaggtct cgggtaccgt
    18241 ttcgaggcct gagcgggcag ggaatgcaga agaggccggt cccttcgggg accggcctct
    18301 tctgcatgca gagcggatgt caggacgcgg gcgttcctgt cggcgtcggc gtcgcgggct
    18361 cgaccgaggg cttcggcgag gggacgagat ccttgaggta ggggagtgtg ccgtcgagca
    18421 cgggcacctg agtgggctcg gcgtcgtcgt cgccggagcg gaacagcacc tcgacggtgg
    18481 cgcccggctt cacgtccagg ttctcgaaga gcagcgggtc ctcgtcggcg cccaggctca
    18541 cgtgctcgcc cgcgggcacg cgcacctccc gctgcttgcc gtccacgtcg atggtgaggg
    18601 tggcgctctc gtcggtgtca ttgaccagca cggccacgag gttcccggcg gagccgtcct
    18661 cgtcggcgac gatgagagcg ttgcgcacga tgaccggacc gccggtcgac gagacgtcca
    18721 cgccgtcgga gatgttcttg atgtccaccg tgttctgcgg ggtcatgaac gtgcagccgg
    18781 tggcgccgag tgcgatcgtc acgccgagcg cggcggcggc gatgaggcgc ggagcgaggg
    18841 acgcagcaga gcgcgagttc acggatcctc ctgagatgcg acgggtgtcc tgcctcagtc
    18901 tagtgggttc gcgcccgttc cccgggaccg gcggaccgcc gcacggcgtc caccggcgcg
    18961 aaccttagta cataacggct gtggtatcct ggagattgcc gaaaggacac gtttttatgc
    19021 tttttgaggt tggcgaaacc gtcgtctatc cgcaccacgg ggctgcgacg atcatcgagg
    19081 tcaaggaccg cgtgatcaag ggggagacga agaagtacct gaagctgaac gtcacgcagg
    19141 gggatctcat catcgaggtt cctgctgaga acgtcgacct ggtcggcgtc cgcgacgtga
    19201 tcggccagga gggtctggac cacgtcttcg aggtgctgcg cgcgccgttc accgaggagc
    19261 cgaccaactg gtcgcgtcgc tacaaggcga acctcgagaa gctcgcgtcg ggcgatgtca
    19321 tcaaggtgag cgaggtcgtc cgcgacctgt ggcgccgtga ccaggaccgc ggcctgtccg
    19381 caggtgagaa gcggatgctg gccaaggccc gtcagatcct cgtctcggag ctggctctcg
    19441 ccgagaagat cgacgaggac aaggccggcg gcctgctcga cgaggtgctc gcttcctgag
    19501 cgcgatcgac tgattccacg aagagggcgg atgctgcggc atccgccctc ttcgcgttct
    19561 cggacgcctg cggccgcgcg gtagcgtgtg agcgtgactc cgtactcggc ccccacgacg
    19621 gcgatcatca tcgtcgccgc cggttccggc acgcgcctcg gcgccggcgt gccgaaggcg
    19681 ctcgtcgaca tcgacggccg caccgtgctg aggcacgcgc tcgaaggcgt gttcgccgcg
    19741 cagccgatgc acgtcatcct cgtcgcgcca cccgggcacg agcaggcggc ggcggacgag
    19801 ctcgaggacg tcaacagcga gcacgactgg cgggccacgg tcgtcgtcgg cggcgagacg
    19861 cggcagcagt cggtggcggc aggccttgcc gctctcgggg acggtatcga ggtcgtcctc
    19921 gtgcacgatg ccgcccgcgc cctgacgccc tccgcgcaga tcgatgcggt cgcggccacc
    19981 gtgaccgcgg atgccggcgt gatccccgcc ctcccagtgg tcgacaccct gaagcgcgtc
    20041 gaaggggatg ccgtgctcga ggccgtcgac cggtcggtcc tggccgcggc gcagactccg
    20101 cagggattcc ctcgcggacc gctcgaggcc gcatacgccc gggcgctcga gacgggggag
    20161 gaccacaccg acgacgcggc cctgttcg
//
LOCUS       DATMSX010000568         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_9466,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000568 DATMSX010000000
VERSION     DATMSX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9466"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VN041_18115"
     CDS             <1..607
                     /locus_tag="VN041_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWU31001.1"
                     /translation="LLTRTGPLLPQQRVLLYAPTWRDGAADPAVPDAAQWVRIVRMLE
                     QHDAILFIRSHPLGEGSYSPPWSSGRVRMLNSELLPDVTPALPRVDLLVTDYSSMAYD
                     VGLLAIPVVYLAPDVEDYARERGFYGRYEDVAGDDYAHDWDGAIEQLDAVLSDDDVRA
                     ERAERSAALSEDMHAYRDGRNAHRVYRSIRARGIPAPKGAR"
     gene            604..2040
                     /locus_tag="VN041_18120"
     CDS             604..2040
                     /locus_tag="VN041_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWU31002.1"
                     /translation="MTTARIDEQTQTLIVAGTGPRPASASLTGSRAQVTAKVTGGGRT
                     WKATFPLRASRWGGAELPLPAGEYQLSAEGEDLSEVDIEPTLLPILRVEARGPVVTIA
                     APLDPVYETAEGQRTLEERYVSQIGAGENAVFFESFYGQTSGCNPRAIDRVLAERAPS
                     VTRYWAVTDLSVEVPEGAVAVVEGSPEWWRARADARMLIVNDWLRRRFVRKPGQRVLQ
                     TWHGTPLKRLALHRPGFDPRRMAAVVKESRRWDALLAQNVYAARVLKKAYAFFGKPIW
                     TDGYPRNDLLITGDPAPVREALGIGPDERVLLYAPTWRDDRSEMVDFVDPEKLASKTD
                     SVVLVRGHSRTIKPGRDRAGSRVIDVTGYPETSRLLLIADALITDYSSVMFDFSVTGR
                     PMYFLVPDLEHYRGELRGFYFDLAERAPGPLVRSQEELIRALADAEGPDAYREHYAAW
                     TSQFNRLDDGGAADRVVSRILDQGWLTA"
     gene            complement(2077..>2786)
                     /locus_tag="VN041_18125"
     CDS             complement(2077..>2786)
                     /locus_tag="VN041_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWU31003.1"
                     /translation="DLRADGRTPFQSAVARLYNSPVGLGGGAYHGGAPEGEAESAYLG
                     VMRREVLDEVGLFDESIRRGEDWELNLRIRQAGHLVWFDPALSVTYWPRESWWRLARQ
                     FRATGAWRGELVRRYGRSNSLRFFAPPALVVLTALAVIVGILQLTGAVHGIASLIASA
                     IYLPLVAYVLLVLAVGLGGLGIARGPRGLHERLWTLAVLPTMHFAWGLGFLAGVLRGA
                     GDTVDASRLGSQNTPLP"
BASE COUNT          414 a          986 c         1006 g          380 t
ORIGIN      
        1 cctgctcacc cgcaccggtc cgctgctgcc acagcagcgc gtgctgctgt acgcgcccac
       61 ctggcgagac ggcgcagccg accccgccgt gccggacgcc gcgcagtggg tgcggatcgt
      121 gcggatgctg gagcagcacg acgccatcct gttcatccgc tcgcatccgc tgggcgaggg
      181 atcgtactcg ccgccgtggt ccagcggcag ggtccggatg ctgaactcgg agctgctgcc
      241 cgacgtcacc cccgcgctgc cacgggtgga cctgttggtg acggactact cctcgatggc
      301 ctatgacgtg ggactgctgg ccatccctgt ggtgtacctg gcgccggatg tcgaggacta
      361 tgcgcgcgag cgcggcttct atgggcgcta cgaggatgtc gcaggcgacg actacgctca
      421 cgactgggac ggcgcgatcg agcagctcga cgccgtgctg agcgacgacg acgtgcgcgc
      481 ggagcgtgcg gagcggtcgg cggcgctcag cgaggacatg cacgcctacc gcgacgggcg
      541 caacgcccac cgcgtctaca ggtccatccg cgcgcgaggg atccccgcgc cgaagggagc
      601 acgatgacca ccgctcgcat cgatgagcag acgcagacgc tgatcgtcgc gggaaccgga
      661 ccgcgcccgg cgtccgcgtc actgaccggg tcgcgggcgc aggtcacggc gaaggtgacc
      721 ggcgggggca ggacctggaa ggcgacgttc ccgctgcgcg cctcgcgctg gggcggcgcg
      781 gagctgcccc ttcccgcggg cgagtaccag ctttccgccg agggggagga cctctccgag
      841 gtcgacatcg agcccacgct gctgccgatc ctgcgcgtgg aggcgcgggg tcccgtcgtg
      901 acgatcgccg cgccgctgga cccggtgtac gagactgccg aggggcagcg caccctcgag
      961 gagcggtacg tgtcgcagat cggcgccggc gagaacgcgg tgttcttcga gagcttctac
     1021 gggcagacct ccggctgcaa cccgcgggcc atcgatcggg tgctcgccga gcgggcaccg
     1081 tcggtgacgc ggtactgggc cgtcaccgac ctgtcggtgg aggtgcccga gggcgccgtc
     1141 gccgtcgtcg agggcagccc ggagtggtgg cgggcacggg ctgacgcgcg gatgctgatc
     1201 gtcaacgact ggctgcggcg gcgcttcgtg cgcaaacccg gccagcgggt gctgcagacc
     1261 tggcacggca cgccgctcaa acggctcgct ctgcaccggc cgggcttcga cccccggcgg
     1321 atggccgccg tggtgaagga gtcccgccgc tgggacgccc tgctggcgca gaacgtgtat
     1381 gccgcgcgcg tgctgaagaa ggcctacgcg ttcttcggca agccgatctg gacggacggc
     1441 tacccgcgca acgacctgct catcaccggg gatcccgcac ccgtgcgcga ggcgctcggc
     1501 atcggacccg acgagcgcgt gctgctgtac gcgcccacct ggcgcgatga ccgctccgag
     1561 atggtggact tcgtcgatcc ggagaagctg gcgtcgaaga ccgactcggt ggtgctggtg
     1621 cgcggacact cccgcacgat caagcccggt cgcgacagag cgggctcccg tgtcatcgac
     1681 gtgaccggtt accccgagac ctcgcgactg ctcctcatcg cggatgccct gatcaccgac
     1741 tactcgtcgg tgatgttcga cttcagcgtg accgggcggc cgatgtactt cctggtcccc
     1801 gacctggagc actaccgggg cgagctgcgc ggcttctact tcgacctcgc cgagcgcgcc
     1861 cccgggccgc tcgtgcgctc gcaggaggaa ctgatccgtg cgctggcgga tgccgagggc
     1921 cctgacgcgt atcgcgagca ctacgcggca tggacctcgc agttcaatcg tctcgacgac
     1981 gggggagccg cggatcgggt ggtctcccgc atcctcgacc agggctggct gaccgcctga
     2041 gcgaccgccg ggtccccggg cccttgatgg ggctcgtcag gggagcgggg tgttctgcga
     2101 cccgagacgc gaggcgtcca ccgtgtcgcc ggcgccgcgg agcacgccgg cgaggaaccc
     2161 gagaccccag gcgaagtgca tggtgggcag cacggcgagg gtccacagcc gctcgtgcag
     2221 cccgcgcggt ccccgtgcga tgccgagacc gcccaggccg acggcgagca ccagcaggac
     2281 gtacgcgacc agcggcaggt agatcgcgct cgcgatgagc gaggcgatgc cgtgcacggc
     2341 gccggtcagc tgcaggatgc cgacgatcac cgccagcgcg gtgagcacca ccagcgcagg
     2401 cggggcgaag aagcgcaggc tgttgctgcg cccgtagcgg cgcaccagct caccgcgcca
     2461 ggcgcccgtc gcgcggaact gacgggccag acgccaccag ctctcccgcg gccagtacgt
     2521 gaccgacagt gccggatcga accacaccag gtggccggcc tgacggatgc gcagattcag
     2581 ctcccagtcc tcgccgcggc ggatgctctc atcgaacagg ccgacctcgt ccagcacctc
     2641 gcgccgcatc acgccgaggt acgcggactc cgcctcgccc tcgggcgcac cgccgtggta
     2701 cgcgccgcct ccgagtccga cgggggagtt gtacaaccgc gccacggcgc tctggaacgg
     2761 ggtccggccg tcggcgcgca ggtcga
//
LOCUS       DATMSX010000569         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_160701,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000569 DATMSX010000000
VERSION     DATMSX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160701"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VN041_18130"
     CDS             <1..733
                     /locus_tag="VN041_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWU31004.1"
                     /translation="LVGGVPGGGEQPAATGENTLSECDTGQDANTNDACRMAGAQVLL
                     DDYWSKHVDGYVKPSLTIVDGQTQTQCGTASNAVGPFYCPPEQGVYLDPTFFQLMRQQ
                     FGASAGELAQLYIVGHEWGHHIQNITGVMQKYPDNGTGPTSNGVRTELQADCYAGAWL
                     KDAVTQKDDNGQNYLQPYTDAQLKDAVNAAYTVGDDHIQEQSGFVNPESFTHGTSEQR
                     EYWFANGYKNGLGVCDTFQVAGDKL"
     gene            817..1782
                     /gene="pip"
                     /locus_tag="VN041_18135"
     CDS             817..1782
                     /gene="pip"
                     /locus_tag="VN041_18135"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HWU31005.1"
                     /translation="MELDGLYPPIEPYETGDLLVGDGNRIHWEQSGNPDGKPVVFLHG
                     GPGGGTSPWQRRFFDPEVYRIVLFDQRGCGLSTPHASEPDADLRYNTTWHLVADIELL
                     RKNLGIPKWQVFGGSWGSTLALAYAQSHPEAVTELVLRGIFTLRRHELEWFYEGGAAA
                     LFPDLWADYEAVIPPVERGRMMEAYHRRLFDADPEVHVPAAVAWTKWEASTVTLRPDP
                     EHVDEATDAAHATAFARIENHYFVNGGWLEEGQLIENVGVIRDIPAVIVQGRYDVCTP
                     MMTAWDLHTAWPEAELVIVDDAGHSASEPGIAAALRAATDRFASL"
BASE COUNT          348 a          706 c          680 g          271 t
ORIGIN      
        1 cctcgtcggc ggcgtgcccg gcggcggcga gcagcccgcg gcgaccggcg agaacaccct
       61 ctccgaatgc gacaccggcc aggacgcgaa cacgaacgac gcctgccgca tggccggcgc
      121 acaggtgctg ctcgacgact actggagcaa gcacgtcgac ggctacgtga agccctcgct
      181 gacgatcgtg gacggacaga cccagacgca gtgcggcacg gcatccaacg ccgtcggtcc
      241 cttctactgc ccgcccgagc agggcgtcta cctcgacccg accttcttcc agctcatgcg
      301 ccagcagttc ggagcgtccg cgggcgagct cgcccagctg tacatcgtcg ggcatgagtg
      361 gggtcaccac atccagaaca tcaccggggt catgcagaag taccccgaca acggcaccgg
      421 ccccaccagc aacggcgtgc gcaccgagct gcaggccgac tgctacgcgg gcgcctggct
      481 gaaggacgcc gtcacgcaga aggacgacaa cggccagaac taccttcagc cgtataccga
      541 cgcgcagctg aaggacgccg tgaacgccgc ctacaccgtg ggcgacgacc acatccagga
      601 gcagtccggc ttcgtgaacc ccgagagctt cacccacggc accagcgagc agcgtgagta
      661 ctggttcgcc aacggctaca agaacggtct cggcgtctgc gacacgttcc aggtcgcggg
      721 cgacaagctg tagtcacagg cggttctccc acgcggtata ccggatccgg gatacggtga
      781 aggggcccga gccacgggca ctcgggggag tgaagcatgg aacttgacgg actgtatccg
      841 cctatcgaac cgtacgagac cggtgatctg ctggtcggcg acggcaaccg catccactgg
      901 gagcagagcg ggaaccccga cggcaagccc gtcgtcttcc tgcacggcgg tcccggcggc
      961 ggcacctcgc cctggcagcg gcgcttcttc gatccggagg tgtaccggat cgtgctgttc
     1021 gaccagcgcg gatgcggtct cagcacgccg catgccagcg agccggacgc cgatctgcgc
     1081 tacaacacga cctggcacct ggtcgccgac atcgagctgc tgcgcaagaa cctcggcatc
     1141 ccgaaatggc aggtgttcgg cggatcgtgg gggagcaccc tcgcgctcgc gtacgcgcag
     1201 tcgcatccgg aggccgtgac cgagctggtg ctgcgcggca tcttcacgct gcgccggcac
     1261 gagctggagt ggttctacga gggcggcgcg gccgcgctgt tccctgacct gtgggccgac
     1321 tacgaggcgg tcatcccgcc cgtcgagcgc ggccggatga tggaggcgta ccaccgccgc
     1381 ctgttcgacg ccgacccgga ggtgcacgtg ccggcggcgg tcgcgtggac gaagtgggag
     1441 gcgtcgaccg tgactctgcg cccggatccc gagcacgtcg acgaggcgac ggatgccgcg
     1501 cacgccacgg cgttcgcgcg catcgagaac cactacttcg tcaacggcgg ctggctcgag
     1561 gagggccagc tgatcgagaa cgtcggcgtc atccgcgaca tccccgcggt gatcgtgcag
     1621 gggcgctacg acgtgtgcac gcccatgatg accgcctggg atctgcacac cgcctggccc
     1681 gaggccgagc tggtgatcgt cgacgacgcc gggcactcgg catccgagcc gggcatcgcc
     1741 gcagcgctgc gcgccgcgac cgaccgcttc gcctcgctct gaccccgaac cgcgccgagc
     1801 ccgcgctgag gttcgatgcg cgggtcgcgt tcgtcctcgc ccctgatcag cgtccacgcc
     1861 cccgctcatt cacgtgaatc agcaggggcg aggacgacca gcaggggcga ggacggaacg
     1921 gtgtcaggcg cgcagcgcct ggagcagcgt cttcacgagg ccgagcacgc cgccgcgggc
     1981 gcggaagcgc gtgaggccct cgctc
//
LOCUS       DATMSX010000570         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_84324,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000570 DATMSX010000000
VERSION     DATMSX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84324"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VN041_18140"
     CDS             <1..137
                     /locus_tag="VN041_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWU31006.1"
                     /translation="APSGPTGGHGLVGMRERAQLVGGTLNAQREGGFFVVRATLPIGA
                     "
     gene            240..875
                     /locus_tag="VN041_18145"
     CDS             240..875
                     /locus_tag="VN041_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU31007.1"
                     /translation="MIKVVLVDDQALFRAGVRMLVGSQPDMDVVGEAGDGREALDVVA
                     RTRPDVVLMDIRMPVMDGLTATAELLKQPNAPRIVMLTTFDLDEAAARAIQQGASGFL
                     LKDADPEFLLAAIRTVESGSSVIAASATRELFAHFTSETKPVPPSYADLTDREREIFA
                     LAARGLSNSEIATREFLSEATVKTHISRILTKLALRDRVQLVVFAFEHGLA"
     gene            complement(888..1742)
                     /locus_tag="VN041_18150"
     CDS             complement(888..1742)
                     /locus_tag="VN041_18150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWU31008.1"
                     /translation="MEIHQLRYAVAVAETGSFTAAAEAVNVSQSGVSTQVQKLERELG
                     VALFDRGARRVTLTADGERMLPAIRESLRALGEIRTTAADLRGLLLGSLRVGTVSGLT
                     WPRLFDALGELHRAHPGLEVRLHEDTSEDLVSALRHDELDIAVVAWAQHPPAGLETAT
                     LFDDPLVAVVAPDHPWASRRSIRPAELDMVDVISLPRGTGDRAALAQLRPALEPRWEV
                     STPWFIERLAARGLGVGVVSDATRRRWEEVRAVPVDAPDVRSRLGIAWRPSPPRAAEA
                     LRALLVAP"
     gene            1813..2286
                     /locus_tag="VN041_18155"
     CDS             1813..2286
                     /locus_tag="VN041_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWU31009.1"
                     /translation="MTTTDQPVPVLVRLDIDTVAPHFSKALAHLDNAMVRELDAVGFD
                     TRLRELVRLRASQLNGCSYCVDLHTGDAQKAGDSLARIAAVAVWRESSLFTARERAAF
                     TLTESITRASETRVPDADWAAAALVFSPEELGALVSLIVTINAWNLVGVSTHAWV"
     gene            2381..3142
                     /locus_tag="VN041_18160"
     CDS             2381..3142
                     /locus_tag="VN041_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU31010.1"
                     /translation="MEITTSDLGLAARVQHLTKSYGAGAAGVRALDDISVGIRRGQFT
                     AIMGPSGSGKSTLMHIMAGLDAPTEGRAWIGDTEITGLNDLELTILRRRRVGFIFQAF
                     NLVPTLDALGNILLPFELDGRRPSVIEKARIDGLVERLGLATRLNHRPHELSGGQQQR
                     VAIARALATAPDLVFADEPTGNLDSRSGREVLQLLASASREHGQSIAMVTHDAIAASH
                     ADRVLFLGDGRITADHPQQSAEQIAAYMLAAEVAA"
BASE COUNT          467 a         1177 c         1078 g          440 t
ORIGIN      
        1 ccgcgccctc gggcccgacc ggcggacacg ggctggtcgg gatgcgtgag cgcgcgcagc
       61 tggtcggcgg aacgctgaac gcgcagcgcg agggcggctt cttcgtcgtc cgagcgacgc
      121 tcccgatcgg tgcgtaggcg ccggcggtac cgcgaccgtg cggtaatcct ggatgtcgcg
      181 gtcaccgcga cccgccaaga tggatgccgg ggttcgttcc ccggccagga ggggtcatca
      241 tgatcaaggt cgtgctcgtc gacgaccagg cgctgttccg cgcgggggtg cggatgctcg
      301 tcggctcgca gcccgacatg gacgtcgtgg gggaggccgg cgacggccgc gaggcgctcg
      361 acgtcgtcgc gcgcacccgg cccgacgtcg tgctgatgga catccggatg ccggtgatgg
      421 acggcctcac ggccacggcc gagctgctga agcagccgaa cgctccccgc atcgtcatgc
      481 tcaccacgtt cgacctcgac gaggccgccg cccgcgccat ccagcagggc gccagcggat
      541 tcctgctcaa ggacgccgac cccgagttcc tgctggccgc catccgcacg gtcgaatccg
      601 ggtcgagcgt gatcgcggca tccgccaccc gcgagctgtt cgcgcacttc acctccgaga
      661 cgaagccggt gccgccgagc tacgccgacc tcacagatcg cgagcgggag atcttcgctc
      721 tcgcggcgcg ggggctgtcg aactccgaga tcgcgacgcg cgagttcctc agcgaggcga
      781 ccgtgaagac gcacatcagc cgcatcctca ccaagctggc tctgcgcgac cgggtgcagc
      841 tggtcgtctt cgccttcgag cacggcctgg cctgagcacg gcgtctctca gggcgcgacg
      901 agcagagcgc gaagcgcctc tgcggcgcgt gggggcgagg ggcgccacgc gatgccgagc
      961 cgcgagcgca cgtcgggcgc gtccacggga acggcccgga cctcctccca gcgtctccgc
     1021 gtggcgtcgc tgacgacccc cacgcccagg ccacgtgcgg cgagccgctc gatgaaccac
     1081 ggggtcgaca cctcccatcg cggctccagg gcgggtcgca gctgcgcgag ggcggcgcgg
     1141 tctccggtgc cgcgcggcag ggagatcacg tcgaccatgt cgagctcggc ggggcggatg
     1201 ctgcgccgtg acgcccaggg gtggtccggt gcgaccacgg cgaccagcgg gtcgtcgaag
     1261 agggtggcgg tctcgagccc agccggcgga tgctgcgccc aggcgaccac ggcgatgtcc
     1321 agctcatcgt ggcgcagcgc ggagacgagg tcctccgagg tgtcctcgtg cagccggacc
     1381 tccagcccgg ggtgcgccct gtgcagctcc ccgagcgcgt cgaacagccg cggccaggtc
     1441 agccccgaca ccgttccgac gcgcaacgat cccagcagca gtccccgcag gtccgcggcg
     1501 gtcgtgcgga tctcgccgag ggcgcggagc gactcgcgga tcgccggcag catccgctcc
     1561 ccgtcggcgg tgagcgtcac gcgacgcgcc ccgcgatcga acagggcgac accgagctcc
     1621 cgctcgagct tctgcacctg cgtgctgacg cccgactggc tgacgttgac cgcctcggcg
     1681 gccgcggtga acgagccggt ctcggcgacg gcgacggcgt agcgcaactg atggatctcc
     1741 atactcagaa cagatgatag cgatctgaat cagctgttgg acagatgact catctcgcgt
     1801 gaggctggat gcatgaccac gacagatcag cccgtccccg ttctcgtccg cctggacatc
     1861 gacaccgtcg ccccgcactt ctcgaaggcc ctcgcgcatc tcgacaacgc gatggttcgg
     1921 gagctggacg ccgtcggctt cgacacccga ctccgcgagc tggtgcgcct gcgcgcctcg
     1981 cagctgaacg gatgcagtta ctgcgtcgac ctgcacaccg gcgacgcgca gaaggccggc
     2041 gactccctcg ccaggatcgc cgcggtggcc gtctggcgcg agtcgtcgct cttcaccgcg
     2101 cgcgagcgcg cggcgttcac tctcaccgag agcatcaccc gggcatccga gacccgcgtg
     2161 cccgacgccg actgggccgc ggccgcgctg gtcttctcgc cggaggagct gggcgcactc
     2221 gtctcgctga tcgtcacgat caacgcctgg aacctcgtcg gcgtctccac gcacgcctgg
     2281 gtctgaggct cgcccgccgc ggggatcatc ctccagatgt acgcgggatg ctccccgcgg
     2341 gcgatgcggc gccgtggagg ccgcgaatag cgtcgatgac atggagatca cgacttcgga
     2401 cctcggcctc gccgcacgcg tccagcacct caccaagtcc tacggcgcgg gggccgccgg
     2461 agtgcgcgcc ctcgacgaca tcagcgtggg catccgccgc ggccagttca cggccatcat
     2521 gggtccgtcc ggatccggca agtccacgct catgcacatc atggcgggtc tcgacgcacc
     2581 gaccgagggg cgcgcgtgga tcggtgacac cgagatcacc ggcctgaacg acctcgagct
     2641 gaccatcctg cgccgtcgcc gcgtgggctt catcttccag gcgttcaacc tcgtccccac
     2701 cctcgatgca ctcggcaaca tcctgctgcc gttcgagctc gacggccgcc ggccctccgt
     2761 catcgagaag gcccgcatcg acggcctggt ggagcgcctg ggcctggcca cccgcctgaa
     2821 ccaccgcccg cacgagctgt ccggcggtca gcagcagcgc gtcgcgatcg cccgtgcgct
     2881 cgccaccgcg cccgacctcg tcttcgccga cgagccgacc ggaaacctcg actcccgcag
     2941 cggacgcgag gtgctgcagc tgctggcatc cgcgagccgc gagcacggcc agtccatcgc
     3001 catggtcacg catgacgcga tcgccgcgag ccacgccgac cgcgtgctgt tcctcggcga
     3061 cggccgcatc accgccgacc acccgcagca gagcgccgag cagatcgcgg cgtacatgct
     3121 cgccgccgag gtggcggcat gaccgccgtc gcctcgggca cc
//
LOCUS       DATMSX010000571        42607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_27678,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000571 DATMSX010000000
VERSION     DATMSX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 42607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42607
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27678"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1437
                     /locus_tag="VN041_18165"
     CDS             127..1437
                     /locus_tag="VN041_18165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWU31011.1"
                     /translation="MMNQTFTVTDYKGVRNIELSPTGRLVVIAGGNGAGKSSFIDAIT
                     ELFDPKGTKLTPKPIREGAAEARAEYVDHDLDLRISRVWKKDGTAGTLTVEALDGAKY
                     STPSKIVADLLGGAIFDPVAFLNLDEKKQRDALLEKVDLPFDLDALAREKAGAEERRL
                     IAGREVKRLEGALASLPKPTAGTPDEEVSGQDILSEIETAQDIERQRIQTRSRYDEAA
                     NLVADYDQQIEDLKRKREEAADRQEVAWGQLRELPDPIDIAPLRERLAAVDDTNRAVR
                     AARQYFEVSGELQVADADHAAAQADLDRIAARKAEGLAAAKFPVDGLSVDDDGVTFRG
                     IPFSQVNSAMRRRIAFAIATSGDPKLKLVIVKDGDLLDADSLQDIAEVAEERGYTVLV
                     ERDRDESRRIGFTVRDGALDEGLPPIEGIAAAAGLNVTPVGGAR"
     gene            1434..1727
                     /locus_tag="VN041_18170"
     CDS             1434..1727
                     /locus_tag="VN041_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31012.1"
                     /translation="MKVSVTVIGLGGKSRQRRKPTLREIATAVQMLQLAEGAMLHANR
                     DGCFMHGIAAATARRMFIKDIPKRLRDNPDWMHRHVEAPGMRIQARVDDGAHS"
     gene            1724..2329
                     /locus_tag="VN041_18175"
     CDS             1724..2329
                     /locus_tag="VN041_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31013.1"
                     /translation="MSALAVLDRTLADTADPSSWDRAHGRVIGSYQAGAFAKPSSVET
                     YIRQMLAPRDFKGNEATRSGNRWEPMLLAWAGAEPNSLLIHHPDEKQFGATVDGTRGA
                     EIFETKAKHNKVVTGPTPREIRQMAWQLFVLPEYEAVNYTWGELVRAFGPEGWELRRD
                     PQNIRFPRDHPQIVAALNLIVPIAHEVLAGVNAARLERSPF"
     gene            2329..3273
                     /locus_tag="VN041_18180"
     CDS             2329..3273
                     /locus_tag="VN041_18180"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HWU31014.1"
                     /translation="MSTELALPSSVRPESWNADTAAMMEFAGLTWIEQRPDPQNPGQM
                     TPVRCYAPSGIMAAFIAACSRTGLDPTAKQIYAALMGGKWTVLVGIDGMRLVAQRTGE
                     YDGQDPIEWLASEDGAWTTVPPKTPFAARARIYRKGVGRPLEQTVTMAEFGGTNGNWK
                     TRPSHQLGIRAESHALRRQFPMELAGLYTPEDFEGGVDTSDAIAAVDSEPWGDLIAAA
                     EDRAELDAIAERLAACGEGTDKLRAAWLARAGVLSREENTEDADVVPDDAETPSEGGE
                     AAPSAPHPPLSEGLTEEEYETAEAARFDAAMDAEEVRP"
     gene            3270..3941
                     /locus_tag="VN041_18185"
     CDS             3270..3941
                     /locus_tag="VN041_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31015.1"
                     /translation="MSVTYGAAPTALTVRDLAFEEYMHFMYLPVLIPEQDPGTPCLAL
                     RLPDRLEFAAQLVHDIVHYEMCTLGNEFTHVYVTARRGYATPGNPLNRPGWHSDGFGT
                     TDVNYVWTDRYPTLFAEQSFVGISTDHIESARQFEEQVDPSRIRTYPDKTLMRLDPSV
                     IHAAPEIPAPGGERSFFKVSFANARYNLRGNSHNYLLDYDWPMHDRAAVRNDPAHTGQ
                     DGGRA"
     gene            3938..4381
                     /locus_tag="VN041_18190"
     CDS             3938..4381
                     /locus_tag="VN041_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31016.1"
                     /translation="MSEIVDGRTGELRLSAAEQAVTEMLAVPSGIYGVPLTPDQANQM
                     ITETADTIEHIAKVIVILLEDKDRAEEVYLAKFADYMIMHEKSGTQMARQYAIQKTKD
                     ERHQLNLKKEMLRYAEEKAKGVQNRSFALMNIGKRVTAAFFGSTR"
     gene            4378..4728
                     /locus_tag="VN041_18195"
     CDS             4378..4728
                     /locus_tag="VN041_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31017.1"
                     /translation="MSAFTTSAPGSRHAATPPWEKADAGFMAALPGAGAWMATMTDGE
                     LHVIRTQDGGLWHLSVSHTTRLPSWDEIADARYRFVPDRARMAMLLPPRAEWIDVHPR
                     TLHLWEIDDPGSTR"
     gene            4725..4856
                     /locus_tag="VN041_18200"
     CDS             4725..4856
                     /locus_tag="VN041_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31018.1"
                     /translation="MKTAVVRLPWWLDFDRPWMVISAGTIGWFATWAEAQHHATRIA"
     gene            4881..5267
                     /locus_tag="VN041_18205"
     CDS             4881..5267
                     /locus_tag="VN041_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31019.1"
                     /translation="MDEKKKDEKPPSFAAMLAQVRPGTDIEAAETLRKVVAAVKETGK
                     TGSLVIRLDVKAADGGLDAVVVTDKISQKIPEKTRVGAMAFITPSGDLSRTDPSAMPL
                     FTDEDIRTAGGDVDLKTGEIKEAPGA"
     gene            5264..6145
                     /locus_tag="VN041_18210"
     CDS             5264..6145
                     /locus_tag="VN041_18210"
                     /inference="COORDINATES: protein motif:HMM:NF021574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HWU31020.1"
                     /translation="MTTIEDTKTEAAVVADLQRQADGEAHEAIRAGEIYLVPDGDGSL
                     EVVDTDDYAEHPRRKEAKRSVTDATSFVTYVNRHADARTEVYAHTNTSKVVAVIDSHD
                     EAGAYPGWQKHRVSLDLEHSKAWIAWAEADGKLFEQDVFADFIDDRYLDVIDPTAARM
                     IDIARSFQAHTKVTFDSTIREASGDVKLNFTEDTAARAGQKGEIEIPAKIQLALRPYV
                     GGPIYSLWANFRYRLRGGAVLLGFKLERPELILEAAFADIVTEIRDGRTDKRDGVETV
                     VHDGIGDVPIFMGRPSA"
     gene            6254..6649
                     /locus_tag="VN041_18215"
     CDS             6254..6649
                     /locus_tag="VN041_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31021.1"
                     /translation="MSVPIIPDPKPRRNRASARKAGTAFERGQADYLAVALNDDRIDR
                     RVKTGAADKGDIAGVRTLNGARVVIECKNTATLSLPAWLREAEAERINDGARIGVVMH
                     KRRGNADPSQQYVTMTTQTFAALLKGGTL"
     gene            6646..7428
                     /locus_tag="VN041_18220"
     CDS             6646..7428
                     /locus_tag="VN041_18220"
                     /inference="COORDINATES: protein motif:HMM:NF015340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HWU31022.1"
                     /translation="MSAIALSAQLDDLKIITLTDGEEWSARDLMPFAGYDRWENFSKA
                     VNRAIASVNASGLNADDHFRGTTKMVQIGSGARREVEDVQLTRYACYILFQNADGSKP
                     EIAALQQYFAVQTRAAEIAGAPLTDDEIIARALQISTSRVQALESHVAELTPRAEAWD
                     ELASAEGDYAVADAAKILARAGIQTGPQRLFADLARFGWVYRGSDGKARAYARAVDDG
                     YLAEKPQSHHHPRTGELVLDVPQVRVTVRGLERLRSRLGGAA"
     gene            7425..8060
                     /locus_tag="VN041_18225"
     CDS             7425..8060
                     /locus_tag="VN041_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31023.1"
                     /translation="MSAWPETMKVGPIGDWPGELTRDRQASKFSATFSDTLRILRREI
                     YHLVDTKAQQDSAEVLIAIPAGAFRLDGRPYANAKAEHPGIIFSIDSRHGHLSYPCDT
                     FTTWQDNLRAVALALEALRKVDRYGVTKRGEQYRGFLAIEATAAPAGFATADAALTYL
                     EQLVDGWADRREPARLLRGAQRIAHPDSGGAAVTFQRVSLAEAKLREAGIL"
     gene            8057..10690
                     /locus_tag="VN041_18230"
     CDS             8057..10690
                     /locus_tag="VN041_18230"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HWU31024.1"
                     /translation="MTLLTLNTGNDGRPYDDFLREKVAFDRTFGFDVRDEWLSPIMRP
                     GHPDFKPHQADIVKWAVKGGRRAIFARYGLGKSVMQLEILRLIVDYGPARERDFYAPF
                     QEVRRGLIVAPLGVRFDIIADGRDLLDTEVRFVRSTAEVDPTWSGLYVTNYESVRDGK
                     LDLSLFVATSLDEAAVLRSFGSETYQRFLPLFEQVPYRFVATATPAPNRHKELIHYAG
                     FLGIMDTGQALTRFFKRDSSKAGNLKIHPHKRREFMLWLNTWACFIQRPSDLGYSDEG
                     YDLPPLEVLWEEVEVGLLSDQVEKDGQGVLVRTGAKSAVESAREKRHTLDARIARAMG
                     IVTKHYADSAEGLNQIIIWCDLNDEQDALEAGLRARGMSFSSIRGGQSDQEEEQQLRA
                     WLAGETYALIGKPQMLGRGLNLQRCSTAVFVGVTHKYEQTVQAIHRIHRFGQTNACKV
                     HLLYGETESDVRDNLLTKWQEDDALTDTMSDILREFGLNATAVSAELARAMGVERETW
                     GGQDWQIALNDSVVEWRDHVAAESMGLIVTSIPFGGKYEYSANYADFGHVDDNGQFWW
                     QMDYLTPSLYRALMPGRILAIHVKDFPLYGSVTGKGVYSFDTMHAEAIAHYTGHGFDY
                     FGMITVTTDVVRENNQTYRLSYSEMTKDHSKMGVGSPEYVLLFHKPQTNRSRGYADLP
                     VTKAKADYSVGQWQIDAAAEWRTGGDRLLSGDELAALEVGVRSKLFTAQSERTVYDYD
                     AHVALADRLAARNALPGTFASLVPGSWRDDIWTDVLRIDTLNSEQRKREVEMHICPFP
                     LDIPRRLIRMYSNPGELVGDPFSGLGSTVLEAVRQGRRGFGSELNPVSTADSVVYLTR
                     HDNEANAPTLFDLLDLEGSAA"
     gene            10687..10920
                     /locus_tag="VN041_18235"
     CDS             10687..10920
                     /locus_tag="VN041_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31025.1"
                     /translation="MTSPFQTMTKRFQIKAYADDVSEITVDLNHDDFQAVMKVLYALN
                     QSAVAYAPRFRVSEVDAEGTERRMLLDPTEAVR"
     gene            10917..12821
                     /locus_tag="VN041_18240"
     CDS             10917..12821
                     /locus_tag="VN041_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HWU31026.1"
                     /translation="MTPSAATIDQSHDAALSAAFDAAHQVTKVKGYVHPVVPWNGLTV
                     TDLFCGAGGSSSGLVEAGYRVIIAANHWALAIESHQVNHPDTDHSQADISQVDPRYFP
                     TTDILWASPECTNHSIAKGVKRQRAMNEALFELDGTRPLVDEAANRSRATMWDVPRFA
                     EHHMYRAIIIENVVDAAKWVQFPAWLMAMELLGYRHETVWLNSMHAQQGGLPAPQSRD
                     RMYIVFWRQDQASKKNPAPRLGRWTQPLALCLEHGEIRATRAFKKKDPWGRYRAQYVY
                     RCPECWQVVEPGWLAADSIIDWSLPAPRIGDREKPLAEKTRERIRRGIERYWAPLVVK
                     AAGNTYDGVNTGSNYLRVSELDTPLPVQTGTAEHGVAIPSLIVRGEGNTVPAKPASGP
                     LGALTASGSMFVAQPLIVNNLSGADASRSQPVSAALPSLVAGGNHASLLVPVEGRDGL
                     KARPSTDAMRTQTARLVDGLVVPAGGTWRDAATSTAAPLSALTTREADGLVVPLRNHG
                     VAKPTDQPIDTVAANGNHHALIMRNNLGGAEMSTPVTEPLRTLTTGGHQSLLEPILPI
                     SLDVDDAGFRMLEPHEIQAGMGFARDYLLLGTKRDKVKQAGNAVTPPCARDLGLAVAE
                     FLNWSEAVAA"
     gene            12982..13356
                     /locus_tag="VN041_18245"
     CDS             12982..13356
                     /locus_tag="VN041_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWU31027.1"
                     /translation="MIGQRPVKLTAAQEKHAYEVATERDGGRCQRCGRVGTVERDHRQ
                     GRDAYNTVPSNLQCLCPGCHRWKTEHPQQAILDGFGVSRWARPTLWPAWRVAVGWVLY
                     DDEGGWQEISQATADLLMNGGA"
     gene            13377..13730
                     /locus_tag="VN041_18250"
     CDS             13377..13730
                     /locus_tag="VN041_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31028.1"
                     /translation="MRIDSDIAQNPKVLDLVAEHGQRGMAAAFMFVCSIGYSAAHNTD
                     GSIRKAALASIHGTAALARLLVEVGLWEQAEKGWNVANYAEHQPTVATREAIEKARSE
                     AGRRAANARWGNDGA"
     gene            13717..14244
                     /locus_tag="VN041_18255"
     CDS             13717..14244
                     /locus_tag="VN041_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU31029.1"
                     /translation="MTERDALRMRPASDGDATGMHAPMRTRCNVTGRYGTERKSYQDT
                     DENPSVPDARTPVDNRYPSLDPEWEAAFLGCEQERLTTSEIAARFRVSTRTISRWRTH
                     LNRNRLPAPVRRPDSDHELAKRLLDDGCSLAEVGRTVGVAASTIHAWFPGVQGWTRVQ
                     SGEYAAMVRLGGMAA"
     gene            14241..14735
                     /locus_tag="VN041_18260"
     CDS             14241..14735
                     /locus_tag="VN041_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31030.1"
                     /translation="MSERTICPPDHAHGKNSTCRAQHHCKCEDCTTFGREYEFWRQGQ
                     LKAGRPLQVPSIGAIRRLRALQRLGWSQSAIGHAAGHTESWPHSITRQTVISTRTDKE
                     VRAVYDALSMRVPVGTTPNEIAAITRARRYAERQGWPPPLAWDDDAIDLPDNDETNER
                     KAAA"
     gene            14732..14929
                     /locus_tag="VN041_18265"
     CDS             14732..14929
                     /locus_tag="VN041_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31031.1"
                     /translation="MTTTKTALELTPGDTVQTEQGPSHVTSITPDGDSVAIDFKTVAK
                     RIGWGDSFPADRTFELVEEAN"
     gene            14929..15420
                     /locus_tag="VN041_18270"
     CDS             14929..15420
                     /locus_tag="VN041_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWU31032.1"
                     /translation="MAGETVITLVGNLTADPELRYTQNGLPVANFTIASTPKTLDKTT
                     NQWKDGEALFLRCSVWREFAEHVAGSLAKGMRVIAQGRLRQHSYQDRDGNNRTAIELE
                     VDEIGPSLRYATAQVTRAQAPQGGQAGRPSAPVTDEPWATPGSSQSDAWSTTGGFGDE
                     TPF"
     gene            15404..15976
                     /locus_tag="VN041_18275"
     CDS             15404..15976
                     /locus_tag="VN041_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31033.1"
                     /translation="MKLRSERDRYAHQRAQFVSETSEHEMRVLHDEGLYRHLRFQRPG
                     TGIWYWDLITWPGSLAIRGDIGEGHIFTRTDDMLRFFDHGQREHEINARYWAEKLDLG
                     RDSVKGFSTDRFTEWCREHAPQITEDDAMVDHEQAAIQVLDEHEIEWDFEDVESWRDY
                     NHHFLIALHAILWGAKRYHAATARPEGGAS"
     gene            15973..16206
                     /locus_tag="VN041_18280"
     CDS             15973..16206
                     /locus_tag="VN041_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31034.1"
                     /translation="MTYFHDNDAPPRPFAARYNGACAAECEHRIHEGDMVQYVDGQLV
                     HVGCIPDEKPEPEPRPICPSCFMEIALNGACSC"
     gene            16200..16604
                     /locus_tag="VN041_18285"
     CDS             16200..16604
                     /locus_tag="VN041_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31035.1"
                     /translation="MLTYPHVCEALTSLGIDPDMVMAAGATEDGYISTARHQDGTELP
                     SESDARRLYRVPWPSRDAWLKFREAFALDTVANGGTPDPRLADGIEAAADWLEARRDQ
                     TWNGGVRRPSNFGRAAAELRIYAAELREADHG"
     gene            16597..16914
                     /locus_tag="VN041_18290"
     CDS             16597..16914
                     /locus_tag="VN041_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31036.1"
                     /translation="MADELPGFDPPPPPKDAPKGERLPQRERDQLAIAAGIHPATRMP
                     LAGNGKTCGDCAALRLKGHPSGKHWWKCGTPDPRSKNHRGDGRDMTKRWPACIAFTER
                     TEP"
     gene            16911..17141
                     /locus_tag="VN041_18295"
     CDS             16911..17141
                     /locus_tag="VN041_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31037.1"
                     /translation="MSDRRPLVPLPVDRRSNLNWDLRSSELSALDDNARFIIRRMVAE
                     SYQAGYEHGWGAGQDDAHTDKAIARDQEEKLS"
     gene            17138..17662
                     /locus_tag="VN041_18300"
     CDS             17138..17662
                     /locus_tag="VN041_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31038.1"
                     /translation="MRILTVRNPWAEAIIYAGKDVENRARNVAGDYRGPVAIYCAKRV
                     NTLDEWDAFRRVHPAAQRRLAQVVREGLPLSFGKIIGVVDLVDVHPVANPHFHPICHD
                     LTGPNDLAAAAARGDGACSPWAHPEGRWHLVLANPRPLAEPIPYRGALGLRNLAVNDP
                     ITTARILAQIGETA"
     gene            17659..18483
                     /locus_tag="VN041_18305"
     CDS             17659..18483
                     /locus_tag="VN041_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31039.1"
                     /translation="MICITNTDLPGICRCTITGAHKVTCRDHEGWAERPGTCRGCLPR
                     LAEVGHLCKGCYERVVAAVAAWPAFREKLDATEGRAVASFGGGGSALGFANLTLVFLA
                     VDECERHLKSRFGTVDNWVQTAPCAADAIQFSHAAERAWNALPVEPTEERAPAPHRCH
                     NCGFLPTWGNDTRKERGADVVTCRNCGTELARIRPDIERWAGSATCEDQLHADCSNLA
                     CTCQCHDLGARSRPAGAQALWDADQHTISPSTSGRADWSISDALTITPTTKETRAA"
     gene            complement(18572..18940)
                     /locus_tag="VN041_18310"
     CDS             complement(18572..18940)
                     /locus_tag="VN041_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31040.1"
                     /translation="MSVGDHIKSASEGASHLTQRVVDAIEKKVNDVTEAVEGAAATTA
                     RKVRDIAGDNDAEREQEADAVSSDDGDRDASEHWADDGGRGPRPAGRSDASTARTGDE
                     TRARSLKPNHIPSQSPDERG"
     gene            complement(18994..19155)
                     /locus_tag="VN041_18315"
     CDS             complement(18994..19155)
                     /locus_tag="VN041_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31041.1"
                     /translation="MWTVLVILLVVWAVLAVTGFAIEGLLWLAIIGVILFVGTVIFGF
                     VRNRSRKDR"
     gene            complement(19293..19754)
                     /locus_tag="VN041_18320"
     CDS             complement(19293..19754)
                     /locus_tag="VN041_18320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU31042.1"
                     /translation="MDVEALTLVELRPRPKGRYWRVFAHSSEFPAPWWDEEHRVDDSD
                     HWISARRDGVEVARCKFVLDARPLDDTPLGPMPHGQLDILAFEVAVSARRQGIGRATL
                     QAIRKLYPAPRLTALNDDEVSRRFWDGIGWVRHQPTNPFSEDVERVAYSEP"
     gene            complement(19816..20088)
                     /locus_tag="VN041_18325"
     CDS             complement(19816..20088)
                     /locus_tag="VN041_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31043.1"
                     /translation="MESEEEIMREMRLNGGAQRVIEDDIEAAEWRKVARAAARRLGRP
                     VETMRAGRIVVAALKDWPGNELEHQLNQAQMRNVMERMSQHLESGS"
     gene            complement(20098..20307)
                     /locus_tag="VN041_18330"
     CDS             complement(20098..20307)
                     /locus_tag="VN041_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31044.1"
                     /translation="MDEELAELKATVAKLEAVEARRANLIEYRDRQLLAMAEQRRLTW
                     VQMQKIAGLSPRGLQLALERARGRQ"
     gene            20372..20842
                     /locus_tag="VN041_18335"
     CDS             20372..20842
                     /locus_tag="VN041_18335"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWU31045.1"
                     /translation="MSFERYRCPACDHLSVIFDDQPFACQNPTCGIDAAVLREPPAEG
                     QTREQFMREHALVYYLALGNRVKIGTTTDLPSRMKALPHEELLAFEFGSYDVERTRHN
                     QFAHARAAGEWFDRADAELTAWISALRADLEDPSDVPRSASRAVKRRILGLEPV"
     gene            20851..21078
                     /locus_tag="VN041_18340"
     CDS             20851..21078
                     /locus_tag="VN041_18340"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWU31046.1"
                     /translation="MKPWLTTAEAAALFGRSKRTIRLWVHDGRNPKNPTLRIRTETTG
                     RRTLLNSEDLRRVAGFKDAYLDAPTFGRQRV"
     gene            complement(21183..21869)
                     /locus_tag="VN041_18345"
     CDS             complement(21183..21869)
                     /locus_tag="VN041_18345"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HWU31047.1"
                     /translation="MGDMHLDVKVTERMYGYIKTDSSEAFRMSTCPMCGRNQALVIAQ
                     TTGGRVFWLRCSNCGRGLVEVDRTVYPSMKPLAEPQGLPEEVAAAWDEVRSCLGVGAN
                     TSAVLMARKILLHVAVGFGLPEKNERGRAPDFTVAIAYLESEGLITRRMKPWVDRIRK
                     VGNSATHELPAITEGEAADVASFTEQLLKLAYEMDHLMSQPNGGEVEDDGSSNRIPTI
                     LMAPVSGWVS"
     gene            21938..22453
                     /locus_tag="VN041_18350"
     CDS             21938..22453
                     /locus_tag="VN041_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31048.1"
                     /translation="MPRITDAQRAEVLRLHTAGKSRNDIARSVGISAGSVTNICKTAG
                     VGFDRSATKHATEARQVDLAAARIRLAEKMLHRSERMLDDLDGSYLVYNFGGKDNTYE
                     EHRLERPPVEVQRNAVVTAGITFDKLTRIVERESGGTESALGLLDHAAAAFSAAADAL
                     RDEGSTDAGSE"
     gene            22437..23714
                     /locus_tag="VN041_18355"
     CDS             22437..23714
                     /locus_tag="VN041_18355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HWU31049.1"
                     /translation="MLDLSSLTRHLSRAQILSVVDSAKRSLALWYGSVSAGKTIASLI
                     AFLLAVRVAPKSGLIVIVGRTLQTVYQNVIVPLQDPLLFGTVIASQVVYTPGATKAII
                     LGREVMIVGAHNVESVGRIQGATIGLAYVDEATLLPEVFWNMLVTRLRVRGARLLATM
                     NPASRNHWLRRNWLLKADAVDLIAFHFTMLDNPSLEQGYIERMKRSYTGVFYDRFIKG
                     EWTNAEGAVYPMWDPNRHQIRFEDMPPIWRVLGIGIDFGTTHATSGIMAGITREEKPR
                     LVLMDEYRYDPRDHDNRRQAPSTTAAQLKAWMGKDHEPRPSSLVPEFHIIDPAAAHFQ
                     EELFKLGVTTWHADNDVLAGIGTTARLLDNDQMIVTNRCAGWLSEVTEYAWDAKATAN
                     GNDEPVKENDDSLDAGRYVVHSTRQSYEYDIAA"
     gene            23883..25490
                     /locus_tag="VN041_18360"
     CDS             23883..25490
                     /locus_tag="VN041_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31050.1"
                     /translation="MPQIVRIATDTRGESSMPLPADGSIFPPKQLAPGFARMREHGAW
                     YTGDVDELRAIYGGAQSATHQRGGRPMQGGVAGFFSRMWWGRPLASGEERTSLHIPMA
                     SNIATLSSDLINAEPPTFGVAGDRDKTQRDATEHRLDVIMNSPQSAMTQNEAGELEAA
                     LGGTYWVADWDTTIADHVFYRSVDADAAVPTFRGNQLAEVSFWTRIDDGKKVYRHIEH
                     HQPGAIIHALYVSDDSDRIGIRVPLTMRPETAHLAELPGAVHDDLQTVIPTYIDRLTA
                     VYEPNIRKPRKLRRKGTFAAWGRSDFEGNEPLLNAFDETWSSWMRDLKVGRARIFVPE
                     AMIQSQGRGQGGFFDAEREVYALLNALPNQAEGKAIVAQQFEIRVEQHERTAFNLKKE
                     ILQATGYSLSSYGEHTQEAQMTATEVQDRRSDTERTRDKKIRYATLARTRIASICLEL
                     DGIIFRSRGGRPGVDVVAEFPAVSQIDPEKEVRIVSLLDAASAISTETKVRRANPDWD
                     DIQVGKEVAKIQTEKGLTAPDPATFRG"
     gene            complement(25491..25919)
                     /locus_tag="VN041_18365"
     CDS             complement(25491..25919)
                     /locus_tag="VN041_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31051.1"
                     /translation="MAVENDEILAPAYAQYRDFDGSVTADTHGGGTLELANKIGLDPD
                     RFWIVGIDVWGADLETARLNVIAIDREATGIADYASLAAHHENSGPIPVTRFLVHNIS
                     PIEVLKVGLKRLNFQLLSRSLPEGASLNVTLDDDLNYDAD"
     gene            26046..26459
                     /locus_tag="VN041_18370"
     CDS             26046..26459
                     /locus_tag="VN041_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31052.1"
                     /translation="MAREQITHNHIVSRPLPPAVEYPPGHPGELLEAVVQVEVPRRNV
                     HVQWNRTGTTDAAGLETGWLQLGIDITIAEIRDLLKYAEEEAQAAKLSSAAFGDYDTE
                     QHQVRVYSSELSRPEINKAINALRRGRDVAYGKDA"
     gene            26459..26719
                     /locus_tag="VN041_18375"
     CDS             26459..26719
                     /locus_tag="VN041_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31053.1"
                     /translation="MLTPAVRARISAEADRIFAEALTVSPREVGALTASVPRPPFVAR
                     RRSLRCWLGFHQWKYFTLVLFGVIDMGERRRSCERCGKWARA"
     gene            26716..26967
                     /locus_tag="VN041_18380"
     CDS             26716..26967
                     /locus_tag="VN041_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31054.1"
                     /translation="MTLYLPAEGEKVTALLGAELGMVTGVYGIVQWIDDSGEQCIGVL
                     SAPGQTQVTTQGLLVVANAVADTELTTFVMDSLGSADGD"
     gene            26957..27163
                     /locus_tag="VN041_18385"
     CDS             26957..27163
                     /locus_tag="VN041_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31055.1"
                     /translation="MGTDDIHVSPVCDLIEHDVDGGDCPCGPTTKPVPRDDGSMGWVI
                     LHHALDGREFAEHDYTGPAMPRES"
     gene            27353..27424
                     /locus_tag="VN041_18390"
     tRNA            27353..27424
                     /locus_tag="VN041_18390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27385..27387,aa:Gly,seq:ccc)
     gene            27601..28311
                     /locus_tag="VN041_18395"
     CDS             27601..28311
                     /locus_tag="VN041_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31056.1"
                     /translation="MSDDQTTNDETSTDQTSAETDVTTDTATADTADASGESTTDATD
                     QGSTAPETFSKEYVEKLRREAAGYREKAKTDAAAAAEAARSEVAQQIGKALGLVQDDT
                     PPDPAALVAQAETERNQAIEREQSLKRQLAVLTGSDKHDANTAELLDSNAFGSKLKAL
                     DPDADDFASLVDALIKETVDGNPTKFKRVQLAQRTGGGDHSGEGASQLSREQLAALPP
                     AERLKAAKEGRLASLLKN"
     gene            28328..29227
                     /locus_tag="VN041_18400"
     CDS             28328..29227
                     /locus_tag="VN041_18400"
                     /inference="COORDINATES: protein motif:HMM:NF040375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HWU31057.1"
                     /translation="MAGIANFIPELWEAAVQVPFEKALVYGQETVANRDYEGEIQQQG
                     DTVNVTTIADPTIRTYDKNADIEIEDVSDGNVKMVIDQGDYYAFRVNDIDKVQAAGDF
                     QSPALARAAFGLRDKVDKYIASLAYAGADAANRVGRLAVVDAEPEYAGTGQTSAYAVL
                     VKLREKLDDQSVPIDGRFVVVNPAFISALSYDPRFTNASASGSTETLRNGFVKQVQGF
                     DVLVSNNVPTVGGAGADKDDKVIVAGVPAAWSFANQLAETEAIRQEKRFANLVRGLNI
                     YGAKVFESKGLATATVTFAAPAV"
     gene            29286..29870
                     /locus_tag="VN041_18405"
     CDS             29286..29870
                     /locus_tag="VN041_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31058.1"
                     /translation="MDDTTETPAETPEPATPDAPNDEGIASETEDDTTETPAEVVPLT
                     EITLPRRMNVVLSGVRFRFGSARARITPLQRLYLTRRGARFEEVAEDPEPPVEPAAPA
                     DTPMTEAELAEAASREAAPESSAQPVNQSTDETAADAPDDAQTEESETPPAEVPVPDE
                     TWTDEALNALGKERGITWRSNASKATKVAKLTEG"
     gene            29933..31801
                     /locus_tag="VN041_18410"
     CDS             29933..31801
                     /locus_tag="VN041_18410"
                     /inference="COORDINATES: protein motif:HMM:NF017919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HWU31059.1"
                     /translation="MPQFLPDPEVTLDDLIEQLGAGLAARYAQVELVLIEAAARRMRK
                     ILALEQAGEEAAALAARQRALRDAELERAAALAELRRIAQQEVARIRKEGLAQRIVAV
                     ASQAGQAAAAAQLAGVRGRAPRAIGTTAANAVASLTLDLESKLDVLNERITRFPDDVY
                     KQVMSQYSPRVVLGAQTTRAAQAAAVQDFLSQGIGHIDYLRKDGSVHMRMPIGSYAEM
                     VARTSSQRAWEDATVYRLQQSGVNLGTIAGGADACAKCAPWIGAVVSFDGTSGTVAVP
                     SAVDGTMITVDVKGSIGDARAAGWGHPNCRDRVVMYSPGLSNPQAVEHAPAAEKERAE
                     QRRLEREIRAAKRDEAAAMNDTDRKRAEQAVRDAQGNMRDFIKQTGRHRQSYREQLGF
                     ADGLGGPRPEYSARPPKMPPTVQFRAGKGTPVGDAARHAVTQIRSVHTIPQTIEPVSI
                     SRMSELQMRFSPTTLGRYTRADSALILRPDAEVEFTAVHELGHWLDNKLFGNDRMYGS
                     DQVASDPWRRWQAAVFESRSSQALRNVLALSSDPRITPELTATAEYLLDPREQFARSY
                     AQWIAMRAADRTLLDQLSTYTTATHPILRAYHWADDDFEAIGSALDDIFRERGLLK"
     gene            31798..31944
                     /locus_tag="VN041_18415"
     CDS             31798..31944
                     /locus_tag="VN041_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31060.1"
                     /translation="MITPANYHDGITFEEAVDGYLQEGLDRARAEVYARILTSPGDPE
                     LPII"
     gene            32025..32201
                     /locus_tag="VN041_18420"
     CDS             32025..32201
                     /locus_tag="VN041_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31061.1"
                     /translation="MLRITHPHPEEGRQVTFGVEFVDGVATVESLHPERALALTQHGH
                     TITKLSNPRRKKAK"
     gene            32198..32620
                     /locus_tag="VN041_18425"
     CDS             32198..32620
                     /locus_tag="VN041_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31062.1"
                     /translation="MNLRTYATLGDYNDVAEVEWPATDVDTLNKRLRAASIEVDKLTR
                     LSVFAADDDGYPTDPDIADAFTEAACAIVEYWQETDDPTGADAFAGAVKIGSVSLGTT
                     SSSQDSMSDAEKLRRRIGSRAIDILMNAGLVPVAVAHT"
     gene            32629..32973
                     /locus_tag="VN041_18430"
     CDS             32629..32973
                     /locus_tag="VN041_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31063.1"
                     /translation="MTRLRAKHLPHRVTIQQLEGEGAEGDIWADPRPDRPAYVEQKTR
                     LVVDRRATSPTVGQEVTATTFVVILPDDDCQPRSRVTVWSGTTRERTAEVIDSAFFDY
                     HGTPSHVELYLE"
     gene            32983..33333
                     /locus_tag="VN041_18435"
     CDS             32983..33333
                     /locus_tag="VN041_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31064.1"
                     /translation="MGVSASVNLTMNFPSDTELNQRIMRGENLAAERLLALSAAEAPF
                     DLGTLVGSGSVENATSPEDGAAVTYDTPYAARLHEHPEFNFQNGRKGKYVEDPALQHK
                     DELGAIIAKEIADG"
     gene            33326..33739
                     /locus_tag="VN041_18440"
     CDS             33326..33739
                     /locus_tag="VN041_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31065.1"
                     /translation="MADAPEIILNRALAQLLNDHDLAVYRPEGAIGELGIRTDGVMPT
                     TVSEFTLITPLRPIPDGRADLIYRAQIYTRRKGTLLTVRTWAADLRAVLDQAEYTPNI
                     LGISFATEFSVLDFEPDSQGRAAVACTYHFRGRRP"
     gene            33784..34539
                     /locus_tag="VN041_18445"
     CDS             33784..34539
                     /locus_tag="VN041_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWU31066.1"
                     /translation="MADSTLYDTTAPSAGSLVLAHQRLIRLKLGGVFINITGDVNNLA
                     LNPTKIPVPREVYGTKGRSSEDIIGYNYAPSFNVEVVRDPVTKQIVAAQAWFKDLVKA
                     AFSEGAANKREFQLFTDALDEDMPVLQGTFSVAYSEANTGYADKGVVTFTLANDGVVN
                     QITPSPLAGSGVPILESATPTLQAPGKIIKVRGYKLGNAVSATVGGKAVLELRVIDEY
                     TVAILIPATVTGSSPIIVTNAAGASNSLPYQAA"
     gene            34562..35005
                     /locus_tag="VN041_18450"
     CDS             34562..35005
                     /locus_tag="VN041_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31067.1"
                     /translation="MITAEKQGRNLVLTMEGVDHPFVIRPLPGHAGRQITDTYLATMG
                     GRPDVDLLAALQMAVDGAILDGDRWAPRPESEQENFTRIGMELSQDEAEQILMPAFFW
                     QTILGMDGVRAYIEGGEGLAGTLKASGALSVRLGFLVPRTSPSSD"
     gene            35291..38233
                     /locus_tag="VN041_18455"
     CDS             35291..38233
                     /locus_tag="VN041_18455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HWU31068.1"
                     /translation="MFDAGALVFKLQTVGAQVFKQDQADAKAAVEKTGQASKESADKV
                     TGLGTATDATGKKAKAAKGPLDEQAASTKKVGDESDNSSKKQDKQKYSTEQQIAAAKE
                     LSKVLLIAGAAAAAMVTLAVAKNTEFDQAMSQTRAATMATAAEQKKLGEAALDAGADT
                     AYSASEAAAAEEELAKAGLSVSNIVGGSLNGALALAAAGQLQVARSAEIMATTLTQFG
                     LTADKSGHVADVLAAGAGKAQGEVEDLALALTYVGPLAKSAGWSLEETGGTLAYFASQ
                     GILGEKAGTSLRGVLAALQAPSTIAAKTMDQYGLSVYDAHGKMLSASQMAAQLQHAFG
                     GLTDQERNAAMGRIFGNESLTAATLLYNGGAQAISKWTDEVNDSGFAAEQAAMRQDNL
                     AGDIEKLGGAFDTALIRTGSNANDVLRAMVQNVTMLVDMYGDAPEPVQALGLVLGVAT
                     AAILLFAGGAVGARAKFVELKATLDATNASMGKTALVGGAAGLALTGIIAVVGLLMSV
                     QAEGRQIAESYADAIDASTGKVTKAARDIAVENLSKAGAWFTFNWQSAYDAADKLGLS
                     LDTVTDAALGNKKALAELDIYLRAAEEDHDALNQVVEKTGLSQQDALFAVGALKTGID
                     QQNNALKEGKKLHDQKSRAEDEATEVTQTATEAYLDQAGSIDKLQKQLSDLIDTINKA
                     NGVNQDAITSNIDYQETLRTVDEQIAKIKSGADGYARSLDITTKAGSDNKAMLVELAQ
                     KAWDAAEAQLQLDGNTDGFTSRLEASRQKLYDTAIAMGATDAEAAALRDSILAIPSKK
                     EIQMIADTASAQNRIDQFVRLNNGRTVRVHVAVDGSQSYRVGSKTVDSGFANGALVQY
                     NANGNFTPSEHHVAQMLRGGAIRVWNEPETQGETYVPHAMSKRSRSEAIMAATAELFG
                     GVYIPAGAKAFADGGGVSVGPSGGADVVALLRMIAAKLDRPNLSIVNPVSRDAKSDAW
                     EAAQIVGL"
     gene            38234..39043
                     /locus_tag="VN041_18460"
     CDS             38234..39043
                     /locus_tag="VN041_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31069.1"
                     /translation="MFSINGIALDNAPLGWVLRAPTSLLFEMSWERPSLRIPGTDGVV
                     SGLPADRNPVVVPFMVQTPRLNLESLLALVGSNGVVTRTTAPTRQAVYETISVRQVGY
                     GPAEAIVDVTFQLRFPEPAWRDMTPVENTYPVTAAIHDNLSVLPGLSAPVGDALIRFV
                     GPFTYANVESTSLAGRSFATLTRTVLAGQCVVFDAATSVWVQNAFPTQWSLVGTDVTG
                     DSFLGGPRDRFEIGPSFTDPTVRTGRVKVNVNGYGTGTSFGVRGRRAYLDV"
     gene            39053..>42607
                     /locus_tag="VN041_18465"
     CDS             39053..>42607
                     /locus_tag="VN041_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWU31070.1"
                     /translation="MLRVRLMNDDLTRGKYLRAAKVEVTQVSSASATIAVTVSERVAG
                     RMDSPQLVSVEVAGAQIRTNLFMLDEDDSDDADQTKMVTLRGVDYVTWMLQRTLLHWS
                     ASAKNGERQWVQFPSGSTGSATPGYTLNGMLVESKNRGWGANVTWDFTGLKDSAGVAW
                     SLAGDDLWWSAWTLLTPLSKVIEMLAEQGFCEWWSEGGKLRLFRPGTADDHTSIVLGG
                     AGFKSRPVKSSFKNVITNFTVVPEKARNWLYLPNPGASTRFGRLEASISQSGVADHVT
                     ATRLAQPALTAARAAQQQFSFDWDLVEGMPVPGVDFNIGDLVTTNTRAGRAVQRVVGI
                     QFTRESSSTSVRAITGDRLLTDALKRARKSASAVIGDIIGGSGNGLPTSKGPTFPVPA
                     APTGLHVETDASQWGADGSAVTSVTLAWDAVSQAEDGSGVDVSAYEVGIRTDGQRGVV
                     ATTDALEWTATGWPVGVTVQVSVRAVAPNGDRGAWSAEIPVTPTDPARPVKVTGLTVT
                     SQQGSWRADGTAQTAVSLAWDAVSVDTGGHVIPVTYEVQSRLPVQSDANTDQTTVTIT
                     GWEPGKTRYVKVRAVSGNGKGDWSDELAVPVAVPASIVPKPPANLQLVVNIGSWGEGG
                     LAIGAVYVQWDRVTESVDGAPVTIVEYEALDVSSYERLQVFDIDPADLPYALGVFPSK
                     SMAQVAVRALTSTGVWSDFAVLDPPVEVASPTLSVISPSALVTSTGLGGASGRWDGKL
                     AGGVDASTVAGFASVQVETATAAAGPWSPAGSRLARAGTVSVQSQTGATVYFRATAYD
                     TLGRAMGTSTVSSVVVQGVSLDDIPGLEGDLDDIRFTADGKNRVYMSATEPVGDTPTV
                     VKRRNAALNPQQVASGSDREAQPRYSWSRSWTTGIMDHPLGLDTALRVTLTAASQTGA
                     GRGFDWYGNADTAPASSGVWLSAVEVTPGQTMHATVWARTNRNGLPFAFGVRFFDHVT
                     LTWKSTLAYSPVVVSIANGWAMLELSAVVPAGATRATFRGVSNRDSVAFVQNDWMEQT
                     GIMIGPGTYLDGRTAPKPGLGYKWEGTPDASASVEVESAFAPGDLWLQLDPTGASIIS
                     ILVWNGTAWAPQVLYADSIIAAESITSQLVRAGAIEVNHVSPSFGDSLNLQGNGSINL
                     LAGRADAAAEQLTSQQAALDQQSAQLAAAQAAADAAQAG"
BASE COUNT         7559 a        14677 c        14061 g         6310 t
ORIGIN      
        1 cggtcgcggc gacgcgatgc ccgcaggcat gaagtactcc ccgctcgccc cgcggtcgcc
       61 gcggcgtgcg caggcgttca tcgcctccgg cggccgcccg aacgtcgccc tcccgaaggg
      121 tcgtgcatga tgaaccagac cttcaccgtg acggactaca agggcgtccg gaacatcgag
      181 ctctccccca ccggccgcct ggtcgtcatc gccggcggga acggcgcggg caagtcgtcg
      241 ttcatcgacg cgatcactga gctgttcgat ccgaagggca ccaagctcac tccgaagccg
      301 atccgggagg gcgccgccga ggcgcgcgcc gagtacgtcg accacgacct cgacctgcgg
      361 atctcgcgcg tgtggaagaa ggatggcacc gcgggcaccc tgaccgtgga ggcgctggac
      421 ggcgcaaagt actccacccc gtcgaagatc gtcgctgacc ttctcggcgg tgcgatcttc
      481 gacccggtcg cgttcctgaa cctcgacgag aagaagcagc gcgacgccct gctcgagaag
      541 gtcgatctcc cgttcgacct ggatgcgctc gcgcgggaga aggcgggcgc ggaagagcgc
      601 cgcctgatcg cgggtcgcga ggtgaagcgg cttgagggcg cgctcgcgtc gctgccgaag
      661 ccgactgctg gcaccccgga tgaggaggtg tccgggcagg acatcctcag cgagatcgag
      721 acggcccagg acatcgagcg ccagcgcatc cagacgcgga gccggtacga cgaggccgcg
      781 aacctcgtcg ccgactacga ccagcagatc gaagacctca agcggaagcg cgaggaggct
      841 gcggatcggc aggaggtcgc gtggggccag ctgagggagc tgcccgatcc gatcgacatc
      901 gcaccgctcc gcgagcgcct cgcggccgtc gacgacacga accgcgccgt gcgcgccgcc
      961 cgccagtact tcgaggtgtc gggggaactg caggtcgcgg atgcggacca tgccgccgcg
     1021 caggccgacc tcgaccgcat cgccgcgcgg aaggctgagg gtctcgccgc cgcgaagttc
     1081 ccggtcgacg gcctctcggt cgatgacgat ggcgtgactt tccgaggcat cccgttctca
     1141 caggtgaact cggcgatgcg tcgccgcatc gcattcgcga tcgccacctc tggtgacccg
     1201 aagctcaagc tcgtcatcgt caaggacggt gacctgctcg acgctgactc cctgcaggac
     1261 atcgccgagg tcgccgagga gcgcggctac accgtgctcg tggagcgcga ccgcgatgag
     1321 tcccgccgga tcgggttcac cgtccgcgac ggtgcgctcg atgagggtct gccgccgatc
     1381 gagggcatcg cggccgccgc gggcctgaac gtcaccccgg tcggaggtgc gcgatgaagg
     1441 tctcggtcac cgtgatcggt ctcggtggga agtcccgtca gcgccgcaag ccgaccctgc
     1501 gcgagatcgc gaccgccgtg cagatgctgc agctggccga gggcgcgatg cttcacgcga
     1561 accgtgacgg gtgcttcatg cacgggatcg cggcggcgac cgcgcgccgc atgttcatca
     1621 aggacatccc gaagcgactc cgtgacaacc ccgactggat gcaccggcat gtcgaggcgc
     1681 ccggcatgcg cattcaggcc cgcgtcgatg acggagcgca ctcgtgagcg cgctcgcggt
     1741 ccttgaccgc accctcgcgg acaccgcgga cccgtcgtcg tgggaccgtg cacacggccg
     1801 cgtgatcggc tcgtaccagg ccggggcgtt cgcgaagccc agcagcgtgg agacctacat
     1861 ccgccagatg ctcgccccgc gcgacttcaa gggcaacgag gccacccgct cagggaaccg
     1921 gtgggagccg atgctgctcg cgtgggcggg cgccgagccg aactcgcttc tcatccatca
     1981 cccggacgag aagcagttcg gggccacggt cgacggcacc cgtggcgcgg agatcttcga
     2041 gacgaaggcc aagcacaaca aggtcgtcac cgggccgacg ccgcgtgaga ttcgccagat
     2101 ggcgtggcag ctgttcgtgc ttcccgagta cgaagccgtc aactacacct ggggcgagct
     2161 ggtgcgcgca ttcggccccg agggctggga gcttcgccga gacccccaga acatccgctt
     2221 ccctcgcgac cacccgcaga tcgtggccgc cctcaacctc atcgtgccga tcgcacacga
     2281 agtactcgcc ggcgtcaacg ccgcccgcct cgaacggagt cccttctgat gtccaccgaa
     2341 ctcgcactcc caagctctgt ccgccccgag tcctggaacg ccgacacggc cgcgatgatg
     2401 gagttcgccg gcctgacctg gatcgagcag cgcccagacc cgcagaaccc cggccagatg
     2461 accccggtcc gctgctacgc cccgtccggc atcatggccg cgttcatcgc cgcgtgctcg
     2521 cgcaccggcc ttgacccgac agccaagcag atctacgccg ccctcatggg tgggaagtgg
     2581 actgtgctcg tcggcatcga cggcatgcgc cttgtcgcgc agcgcaccgg cgagtacgac
     2641 ggccaggacc ccatcgagtg gctggcctct gaggacggcg catggaccac ggtccccccg
     2701 aagacgccgt tcgccgcccg cgcgcgcatc taccgtaagg gtgtcggccg tcccctcgag
     2761 cagaccgtca ccatggctga gttcggcggc acgaacggca actggaagac gcgcccatca
     2821 caccagctcg gcatccgcgc agagtctcac gccctgcgtc gccagttccc catggaactg
     2881 gcaggcctct acacgcccga ggacttcgag ggtggcgtgg acaccagcga cgcgatcgcg
     2941 gccgtcgact cggagccgtg gggcgatctg atcgccgccg cagaggatcg cgccgaactc
     3001 gatgcgatcg ccgagcgctt ggccgcatgc ggagagggta ccgacaagct ccgcgccgcc
     3061 tggctggccc gcgccggcgt gctctcccgc gaggagaaca ccgaggacgc cgacgtcgtc
     3121 cccgacgacg ctgagactcc ctccgagggc ggagaggcgg ccccctccgc tccccatcct
     3181 ccgctctcgg agggcctgac cgaggaggag tacgagaccg cggaggccgc gcgcttcgac
     3241 gccgccatgg atgccgagga ggtgcgcccg tgagcgtcac ctatggcgcc gcgcccaccg
     3301 ctttgaccgt gcgggacctc gccttcgagg agtacatgca cttcatgtac ctcccggtcc
     3361 tcatccccga gcaggatccg ggcactccgt gcctggcact ccgcctccct gaccgtctgg
     3421 agttcgccgc gcagctggtc cacgacatcg tgcactacga gatgtgcacg ctcggaaacg
     3481 agttcacgca cgtctacgtg accgcgcgtc gcgggtacgc gaccccgggc aaccctctca
     3541 accgtcccgg gtggcactct gacgggttcg gcacgaccga cgtcaattac gtctggaccg
     3601 accgctaccc gactctgttc gccgagcagt cgttcgtggg catcagcacc gaccacatcg
     3661 agtccgctcg ccagttcgag gagcaggtcg atccctcccg gatccgcacc taccccgaca
     3721 agacgctcat gcgcctggac ccgtctgtga tccacgccgc cccggagatc ccggcgcccg
     3781 gcggcgagag gtcgttcttc aaggtgtcgt tcgcgaacgc gcggtacaac ctgcgaggga
     3841 acagccacaa ctacctcctc gactacgact ggccgatgca cgatcgcgcc gcagtccgca
     3901 acgatcccgc gcacacggga caggacggag gccgcgcatg agcgagatcg tcgatggccg
     3961 taccggcgaa ctgcgcctct cggccgccga gcaggctgtg accgagatgc tcgcggtccc
     4021 ctcggggatc tacggcgtcc ccctgacacc ggaccaggcg aaccagatga tcaccgagac
     4081 ggccgacacc atcgagcaca tcgcgaaggt catcgtcatc ctgctggaag acaaggaccg
     4141 ggcggaggag gtgtacctgg cgaagttcgc ggactacatg atcatgcacg agaagtcggg
     4201 tacgcagatg gcccgccagt acgcgattca gaagaccaag gacgagcgcc accagctgaa
     4261 cctcaagaag gagatgctcc gctacgccga ggagaaagcg aagggcgtgc agaaccgcag
     4321 cttcgcgctg atgaacatcg gtaagcgggt caccgcggcg ttcttcggga gcacgcggtg
     4381 agcgcgttca ccacctcggc gcccggctcg cggcacgctg ccacgccgcc ctgggagaag
     4441 gccgatgcgg gcttcatggc cgcactccct ggtgcaggcg cgtggatggc gacgatgacc
     4501 gacggtgagc ttcacgtgat ccgcacccag gacggcgggc tgtggcatct gtccgtgtcg
     4561 cacaccaccc gcctcccatc gtgggacgag atcgcggatg cccgataccg gttcgttccg
     4621 gaccgcgcgc gcatggccat gttgctgccg ccgcgcgccg agtggatcga cgtgcacccg
     4681 cggactctgc acctgtggga gatcgacgat cccgggagca cgcgatgaag accgccgtgg
     4741 tccgcctgcc ctggtggctc gacttcgacc gtccgtggat ggtcatcagc gcgggcacga
     4801 tcggctggtt cgccacgtgg gctgaggccc agcaccacgc gacacggatc gcatagacca
     4861 ccacgagaaa ggacatccag gtggatgaga agaagaagga cgagaagccg ccgagcttcg
     4921 cggcgatgct cgcgcaggtc cggcccggca ccgatatcga ggccgccgag acgctccgca
     4981 aggtcgtcgc cgcggtcaag gagacgggca agaccggctc gctcgtgatc cgcctcgacg
     5041 tgaaggccgc cgacggcggg ctggatgcgg tcgtcgtgac ggacaagatc agccagaaga
     5101 tccccgagaa gacccgtgtc ggagcgatgg cgttcatcac tccctcgggc gacctgtcgc
     5161 gtacggatcc gtccgcgatg ccgctgttca ccgatgagga catccgcacc gccggcggcg
     5221 atgtcgacct gaagaccggc gagatcaagg aggcccctgg ggcatgacca ccatcgagga
     5281 caccaagacc gaggcggctg tcgtcgccga cctgcagcgg caggccgacg gcgaagcgca
     5341 cgaggcgatt cgcgccggcg agatctacct ggtgccggac ggagacgggt cgctcgaggt
     5401 cgtcgatacc gacgactacg ccgagcaccc ccgccgcaag gaggcgaagc gcagcgtcac
     5461 cgacgcgacg tcgttcgtca cctacgtgaa ccggcacgca gacgctcgca cggaggtcta
     5521 cgcccacacg aacacctcca aggtcgtcgc ggtcatcgac tcgcatgacg aggcgggcgc
     5581 gtaccccggc tggcagaagc accgcgtcag cctcgacttg gagcactcca aggcgtggat
     5641 cgcatgggca gaggccgacg gcaagctgtt cgagcaggac gtgttcgctg acttcatcga
     5701 cgaccgctac ctggacgtca tcgacccgac ggccgcacgg atgatcgaca tcgcacggtc
     5761 cttccaggcg cacacgaagg tcaccttcga ctccacgatc cgcgaggcca gtggcgatgt
     5821 gaagctcaac ttcaccgaag acacggccgc aagggctggg cagaagggcg agatcgagat
     5881 ccccgccaag atccagttgg cgctgcgccc gtacgtcggc ggcccgatct acagcctctg
     5941 ggccaacttc cggtaccggc tgcgtggcgg ggctgtcctc ctgggattca agctcgaacg
     6001 ccccgagctg atccttgagg cggcgttcgc cgacatcgtg accgagatcc gcgacggtcg
     6061 caccgacaag cgcgacggcg tggagaccgt cgtgcacgac ggcatcggcg acgtccccat
     6121 cttcatggga cggccgtccg cctgaccggt cgctgcacgt agcaggtgca gcgcccacaa
     6181 gaacggggac cggccgagta gcagtcggcc ggtcccccac ccacacatcg cattccgtag
     6241 caggagactc agcgtgagcg tacccatcat ccccgatccc aagccccgac gaaaccgcgc
     6301 gagcgcgcgc aaggcgggca ccgcattcga gcgtggacag gccgactacc tggccgtcgc
     6361 gctgaacgac gaccgcatcg accgtcgcgt gaagaccggc gccgccgaca agggcgatat
     6421 cgccggcgtc cgcaccctca acggcgcccg cgtcgtcatc gagtgcaaga acaccgcgac
     6481 gctgagtctc ccggcctggc tgcgggaggc cgaagccgag cgcatcaacg acggtgcgcg
     6541 catcggcgtc gtcatgcaca agcgcagggg gaacgctgac ccgtcccagc agtacgtgac
     6601 gatgaccacc cagaccttcg ccgccctcct gaagggcggc accctgtgag cgccattgca
     6661 ctgtccgcgc agctggacga cctcaagatc atcaccctca ccgacggtga ggaatggtcc
     6721 gcccgcgacc tcatgccctt cgcgggctac gaccggtggg agaacttctc gaaggccgtc
     6781 aaccgggcga tcgcgtccgt gaatgcctcc ggtctgaacg ccgacgacca tttccgtggc
     6841 accacgaaaa tggtgcaaat cggctccggc gcccgccgcg aggtcgaaga cgtccagctg
     6901 actcgctacg cctgctacat cctgttccag aacgcggacg gttcgaagcc agagatcgct
     6961 gcgctgcagc agtacttcgc ggtccagact cgtgccgcgg agatcgccgg cgcgccgctc
     7021 acggacgacg agatcatcgc gcgtgccctg cagatcagca cgtcgcgtgt gcaggcgctg
     7081 gaatcgcacg tcgccgagct cacaccgcgc gcggaagcgt gggacgagct cgcgtccgcc
     7141 gagggtgact acgccgtcgc ggatgccgcg aagatccttg cccgcgccgg tatccagacg
     7201 ggcccgcagc gcctgttcgc agacctggcg cggttcggat gggtctaccg agggagcgac
     7261 gggaaggcgc gagcgtacgc ccgtgccgtc gacgacggat acctcgctga gaagccgcag
     7321 tcgcaccacc acccgcgcac gggcgagctg gtgctcgacg tgcctcaggt gcgtgtcacg
     7381 gtgcgcggtc tggaacgtct ccgctcccgc ctgggaggtg ccgcatgagc gcctggccgg
     7441 agacgatgaa ggttggtccg atcggagact ggccgggcga gctcacccgc gatcgacagg
     7501 cgtcgaagtt cagcgccacg ttctcggaca ctctgcggat ccttcgccgt gagatttacc
     7561 acctggtcga cacgaaggcg cagcaggact ccgcagaggt gctcatcgcc atccccgcgg
     7621 gcgcgttccg cctcgacggc cgcccgtacg cgaatgcaaa ggccgagcac ccgggcatca
     7681 tcttctcgat cgactcccgc cacggccacc tgtcgtaccc gtgcgacacg ttcacgacct
     7741 ggcaagacaa cctccgcgcc gtcgctctcg cactcgaagc gctgcgcaag gtcgaccggt
     7801 acggcgtcac gaagcgaggc gagcagtacc gcgggttcct cgcgatcgaa gcgaccgcgg
     7861 cgccggccgg gttcgccacg gcggatgccg cgctcaccta cctcgagcag ttggtcgacg
     7921 gatgggcgga ccgccgcgag cctgcgcggc tgctgcgcgg ggcgcagcgc atcgcgcacc
     7981 ccgacagcgg cggcgccgcg gtgacatttc agcgtgtgtc gctcgctgag gcgaagctgc
     8041 gagaggcggg catcctgtga ctctcctcac cttgaatacc ggcaacgacg gtcgcccgta
     8101 cgacgacttc ctgcgcgaga aggtcgcgtt cgaccgcacg ttcgggttcg acgtgcgcga
     8161 cgagtggctg tcgccgatca tgcggcccgg gcacccggac ttcaagccgc accaggccga
     8221 catcgtgaag tgggcggtca agggcggccg gcgtgcgatc ttcgcccgct acggtctcgg
     8281 gaagtccgtc atgcagctgg aaatcctgcg gctgatcgtg gactacggcc cggcccggga
     8341 gcgcgacttc tatgccccgt tccaggaggt acgccgggga ctgatcgtcg cgccgctcgg
     8401 tgtccgcttc gacataatcg ccgacggccg cgatctcctc gacacggagg tgcgattcgt
     8461 ccggtcgacg gcggaggtgg acccgacttg gtcgggcctg tacgtcacca actacgagtc
     8521 cgtgcgggac gggaagctcg acctgtcgct gttcgtcgcg acgtcgctcg acgaggccgc
     8581 cgtgctgcgc tcgttcgggt ccgagaccta ccagcggttc ctgccgctgt tcgagcaggt
     8641 tccctaccgg ttcgtcgcga ccgcgacgcc ggcgccgaac cgtcacaagg aactgatcca
     8701 ctacgccggg ttcctcggca tcatggacac cggccaggcc ctgacgcggt tcttcaagcg
     8761 ggactcgtcg aaggcgggga acctcaagat ccacccgcac aagcgccgcg agttcatgct
     8821 gtggctgaac acgtgggcgt gcttcatcca gcggccctcg gatctcggct actccgacga
     8881 gggatacgac ctgccgccgc tcgaggtgct ctgggaggag gtcgaggtcg gtctcctgtc
     8941 tgatcaagtc gagaaggacg ggcagggcgt gctcgtacgc accggggcga agtcggctgt
     9001 cgagtcggct cgggagaagc ggcacacgct cgatgcgcgg atcgcgcgcg cgatgggcat
     9061 cgtgaccaag cactacgccg actctgccga aggcctgaac cagatcatca tctggtgcga
     9121 cctgaacgac gagcaggacg cgctcgaagc cgggctccgc gcccgcggca tgtcgttctc
     9181 ctcgatccgt ggcggacagt ccgaccagga ggaagagcag cagctgcgcg cctggctggc
     9241 aggtgagacg tacgcgctga tcgggaagcc gcagatgctc gggcgcggtc tgaacctgca
     9301 gcggtgctcc accgcggtgt tcgtcggcgt gacgcacaag tacgagcaga ccgtccaggc
     9361 gattcaccgc atccaccggt tcgggcagac gaacgcctgc aaggtgcacc tgctgtacgg
     9421 cgagaccgag tccgacgtgc gcgacaacct gctcacgaaa tggcaggagg acgacgccct
     9481 cacggacacg atgagcgaca tcctccgtga gttcgggctg aacgccacgg ccgtgtcggc
     9541 ggagctcgcc cgagcgatgg gcgttgagcg tgagacgtgg ggcggccagg actggcagat
     9601 cgccctcaac gattcggtgg tcgagtggcg ggaccacgtc gccgcggagt cgatgggcct
     9661 catcgtcacg tcgatcccgt tcggtgggaa gtacgagtac tcggctaact acgccgactt
     9721 cgggcacgtg gacgacaacg gccagttctg gtggcagatg gactacctca cgccgtcgct
     9781 gtaccgcgcg ctcatgccgg gccggatcct cgcgatccac gtgaaggatt tcccgctgta
     9841 cgggtcggtc accgggaagg gcgtctactc gttcgacacc atgcacgccg aggcgatcgc
     9901 ccactacacc gggcacggat tcgactactt cgggatgatc acggtcacca ccgacgtcgt
     9961 gcgcgagaac aaccagacct accggctctc gtactcggag atgacgaagg accactcgaa
    10021 gatgggcgtc ggctccccgg agtacgtgct gctgttccac aagccgcaga cgaaccggtc
    10081 ccgcgggtac gccgaccttc ccgtgacgaa ggcgaaggcc gactactccg tcggtcagtg
    10141 gcagatcgat gccgccgctg agtggcgcac gggcggcgat cggctgctgt ccggcgacga
    10201 gctcgccgct ctcgaggtcg gtgtccgctc gaagctgttc accgcgcagt ccgagcgcac
    10261 cgtgtacgac tacgacgcgc acgtcgcgct cgctgaccgc ctggcagcgc gcaacgccct
    10321 ccctggcacg ttcgcgtcgc tcgtgcccgg gtcgtggcgt gacgacatct ggaccgacgt
    10381 actgcgcatc gacacgctca acagcgagca gcgcaagcgc gaagtcgaga tgcacatctg
    10441 cccgttcccg ctcgacatcc cccgccggct gatccgcatg tactccaacc ccggcgagct
    10501 cgtcggtgac ccgttcagcg gactcggctc caccgtcctc gaagcggtcc gtcagggccg
    10561 ccgcgggttc ggatccgaac tcaacccggt gagcacggcc gactcggtcg tctacctcac
    10621 ccgccacgac aacgaggcga acgcacccac gctgttcgac ctcctcgacc tcgaagggag
    10681 cgcggcatga cgtcgccatt ccagaccatg accaagcggt tccagatcaa ggcgtacgcg
    10741 gatgacgtct cagagatcac cgtcgacctc aaccacgacg acttccaggc cgtcatgaag
    10801 gtgctgtacg cgctcaacca gagcgccgtg gcctatgcgc cgcggttccg cgtctccgaa
    10861 gtcgacgctg agggcaccga gcgccggatg ctgctggacc cgacggaggc cgtccgatga
    10921 ccccgtcagc agcgaccatc gatcagtcgc acgacgccgc cctgtcggcc gcgttcgacg
    10981 ccgcccacca agtcaccaag gtcaagggct acgtgcatcc cgtggtgccg tggaacggcc
    11041 tcacggtcac ggatctgttc tgtggtgccg gcggttcgtc cagcggtctc gtggaggccg
    11101 gataccgggt catcatcgcg gcgaaccact gggcgctggc gatcgagtcg caccaggtga
    11161 atcaccccga cacggatcac tcgcaggcgg atatctcgca ggtcgatccg cggtacttcc
    11221 cgacgacgga catcctgtgg gcgtcgccgg agtgcacgaa ccactcgatc gcgaagggcg
    11281 tgaagcggca gcgcgcgatg aacgaggccc tgttcgagtt ggacggcacc cgcccgctgg
    11341 tcgatgaggc cgcgaaccgc tcccgcgcga cgatgtggga cgtgccgcgg ttcgcggagc
    11401 accacatgta ccgggcgatc atcatcgaga acgtcgtgga tgcggcgaag tgggtgcagt
    11461 tcccggcctg gctgatggcg atggaactgc tcggctaccg tcacgagacg gtctggctga
    11521 actcgatgca cgcgcagcag ggcggccttc ccgcgccgca gtcccgcgac cgcatgtaca
    11581 tcgtcttctg gcggcaggat caggcgtcca agaagaaccc ggccccgcgt ctcggccggt
    11641 ggacgcagcc gctcgccctg tgcctggaac acggcgagat ccgcgcgacg cgcgcgttca
    11701 agaagaagga tccgtggggc cgataccggg cgcagtacgt ctaccggtgc cccgagtgct
    11761 ggcaggtcgt ggagccgggt tggctcgccg cggactcgat catcgactgg tcgctgcccg
    11821 cgccgcgcat cggcgacagg gagaagccgc tggcggagaa gactcgcgag cggatccgcc
    11881 gcggtatcga gcgctactgg gcgccgctgg ttgtgaaggc cgccgggaac acctacgacg
    11941 gcgtcaacac ggggtcgaac tacctgcgcg tcagcgagct cgataccccg ctccccgtgc
    12001 aaacaggaac cgcagagcac ggcgtcgcga tcccgagcct catcgtgaga ggcgagggga
    12061 acacggttcc cgcgaagcct gcaagcgggc ccctcggcgc gctgacggca tctgggtcga
    12121 tgttcgtggc gcagccgctg atcgtgaata acctcagcgg cgcagacgcg tcgcggtctc
    12181 agcctgtcag cgctgctctg ccgtcgctcg tcgctggcgg caaccacgcc tcgctgctgg
    12241 tcccggtcga gggccgcgac gggttgaagg cgcgaccctc aacggacgcc atgcgcacgc
    12301 agacggctcg actcgtcgat gggctggtcg tccccgccgg cggaacgtgg cgcgatgccg
    12361 caacgtcgac ggccgctccg ctgtctgccc tcacgacccg cgaggccgac ggcctggtcg
    12421 tgccgctgcg gaatcacggg gtggcgaagc cgaccgatca gccgatcgac acggtcgccg
    12481 cgaacggcaa ccaccacgcc ctgatcatgc gcaacaacct cggcggcgcg gagatgtcca
    12541 cgcccgtgac cgagccgttg cgcacgctga ccacgggcgg ccaccagtcc ctgctcgagc
    12601 cgatcctccc gatcagtctc gacgtggatg acgccggctt ccggatgctc gaaccgcacg
    12661 agatccaggc ggggatgggc ttcgcccgcg actacctcct gctcggcacg aagcgcgaca
    12721 aggtcaagca ggcaggcaac gcggtcacgc cgccgtgcgc acgagatctc gggctcgcgg
    12781 tcgccgagtt cctgaactgg tccgaggcgg tggctgcgtg atgatcctcc gctacgcgcc
    12841 ggacacgcgc gcccacgacg ccccgaagag ccggtgcggg cgtgagtgct gctggacccc
    12901 gatggggcac tccaccgtcc gcgacgggct ccctgcgcac caccccggcg cgtgccactg
    12961 ccacgacaag gagaagggcc gatgatcggg cagcgtccgg tgaagctcac cgccgcccag
    13021 gagaagcacg cctacgaagt cgccacggaa cgtgacggtg ggaggtgcca gcgatgcggg
    13081 cgcgtcggca ccgtggagcg ggatcaccgg cagggccggg acgcgtacaa cacggtcccg
    13141 tcgaacctgc agtgcctctg cccgggctgc caccggtgga agaccgagca ccctcagcag
    13201 gcgatcctcg acggtttcgg ggtgtctcgg tgggcgcgcc cgaccctgtg gcccgcatgg
    13261 agggtcgccg tcggctgggt gctctacgac gacgagggcg gctggcagga gatctctcag
    13321 gccaccgcgg atctgctcat gaacggcggt gcgtgatggc gacaggactc ccctgggtgc
    13381 gcatcgacag cgacatcgcc cagaacccga aggtgctcga tctggtggcc gaacacgggc
    13441 agcgcgggat ggccgcggcg ttcatgttcg tctgctcgat cgggtactcc gcggcccaca
    13501 acacggacgg atccatccgg aaagcagcgt tggcgtcgat tcacggcacc gccgctctgg
    13561 cccggctgtt ggtcgaagtc ggcttgtggg agcaggcgga gaagggctgg aacgtcgcga
    13621 attacgccga gcaccagccg accgtggcaa cacgggaggc gatcgaaaag gccaggtcag
    13681 aagcaggaag acgggcagcg aatgcgaggt ggggaaatga cggagcgtga tgcgctgcgc
    13741 atgcgccccg catccgatgg ggatgcgacg ggcatgcatg cgccgatgcg aactcgatgc
    13801 aacgtaacgg gacggtacgg gacggaacgt aagagctacc aagacaccga tgaaaatcct
    13861 tctgttcctg atgcgcgaac ccctgtggat aaccggtatc cgtcgctcga tcctgagtgg
    13921 gaagccgcgt tcctgggatg cgagcaggag cgtctcacga cgtctgagat cgctgcccgg
    13981 ttccgtgtct cgacgcggac gatcagtcgg tggcgcactc acctgaatcg aaaccgactg
    14041 cccgcgccgg tgcgacgccc cgactccgac cacgagctcg cgaagcgcct cctcgatgac
    14101 ggatgctcgc ttgccgaggt cgggcggact gtcggcgtcg ccgcgagcac gatccacgca
    14161 tggttccccg gcgtgcaggg ctggacgcgt gtccagtccg gcgagtacgc ggccatggtg
    14221 cgactcggag ggatggcggc atgagcgagc gcaccatctg tccgcccgat cacgcccacg
    14281 gcaagaactc cacctgccgg gcccagcacc actgcaagtg cgaggactgc accacgttcg
    14341 gtcgcgaata cgagttctgg cggcaggggc agctcaaggc cggtcggccg ctgcaggtcc
    14401 cctcgatcgg cgcgatccgg cggctgaggg ccctgcagcg cctcgggtgg tcgcagtctg
    14461 cgatcgggca tgccgcgggg cacacggagt cctggccgca cagcatcacg cgtcagaccg
    14521 tcatcagcac gcggaccgac aaggaggttc gggcggtgta tgacgcactg tccatgcggg
    14581 tcccggtcgg caccaccccg aacgagatcg ccgcgatcac cagggcgcgc cgctacgccg
    14641 agcgccaggg atggccaccc cctctcgcct gggatgacga cgccatcgac ctccccgaca
    14701 acgacgaaac gaacgaacga aaggcagcag catgaccacc acgaagaccg cactcgagct
    14761 caccccgggc gacaccgtcc agaccgagca ggggcccagc cacgtcacca gcatcacccc
    14821 ggacggcgac agcgtcgcga tcgacttcaa gaccgtcgcc aagcggatcg gctggggcga
    14881 ctccttcccc gccgaccgca ccttcgagct cgtcgaggag gcgaactgat ggccggcgaa
    14941 accgtgatca ccctcgtcgg caacctcacc gccgatcccg agctgcggta cacgcagaac
    15001 ggactcccgg tggcgaactt caccatcgca tcgaccccga agacgctcga caagaccacg
    15061 aaccagtgga aggacggcga ggccctgttc ctccgctgca gcgtgtggcg cgagttcgcg
    15121 gagcacgtcg ccggcagcct cgcgaagggc atgcgcgtca tcgctcaggg ccgactccgt
    15181 cagcactcct accaggaccg tgacggcaac aaccgaacgg caatcgagct cgaggtcgat
    15241 gagatcggcc cgtcgctccg ttacgcgacg gcgcaggtca cccgcgcgca ggccccgcag
    15301 ggcggccagg cgggccgacc gtccgctccc gtgactgatg agccgtgggc gactcccgga
    15361 agtagccaga gcgacgcatg gagcacgacg ggagggttcg gcgatgaaac tccgttctga
    15421 gcgggaccga tacgcacacc agcgcgcaca gttcgtcagc gagacgtcgg agcacgagat
    15481 gcgggtgctg cacgacgagg gcttgtaccg tcacttgcgc ttccagaggc ctggcaccgg
    15541 catctggtac tgggatctga tcacctggcc gggttcgctc gcgatccgcg gcgacatcgg
    15601 cgaagggcac atcttcacgc ggaccgatga catgctgcgg ttcttcgatc acggccagcg
    15661 cgagcacgag atcaacgcca gatactgggc ggagaagctc gacctcggcc gcgactcggt
    15721 caaggggttc tcaaccgatc ggttcacgga gtggtgccgc gagcacgcgc cacagatcac
    15781 cgaagacgac gcgatggtcg atcacgagca ggctgcgatc caagtactcg atgagcacga
    15841 gatcgagtgg gacttcgagg acgtcgagtc gtggcgcgac tacaaccacc acttcctcat
    15901 cgcgctgcac gcgatcctct ggggcgcgaa gcgctaccac gccgcgacgg cgcgtcccga
    15961 aggcggtgca tcgtgacgta cttccacgac aacgacgccc caccgcggcc gttcgccgcc
    16021 cgctacaacg gcgcatgcgc cgccgagtgc gagcaccgca tccacgaggg cgacatggtc
    16081 cagtacgtcg acgggcaact ggtgcacgtc ggctgcatcc cggacgagaa gccggaaccg
    16141 gagccccgcc cgatctgccc gtcctgcttc atggagatcg cactgaacgg agcctgctca
    16201 tgttgaccta cccgcacgtc tgcgaggccc tcaccagcct tggcatcgac cccgacatgg
    16261 tcatggccgc cggcgcgacc gaggacggct acatctcgac cgcgcgacac caagacggca
    16321 ccgaactgcc gtcagagtcc gacgcccgcc ggctgtaccg cgtgccgtgg ccatcacgcg
    16381 acgcctggct gaagttccgg gaggcgttcg cgctcgacac cgtcgcgaac ggaggcacac
    16441 cagatccacg tctcgccgat ggcatcgagg cggcggccga ctggctcgaa gcccggcgtg
    16501 accagacctg gaacggtggc gtgcgccgac cgagcaactt cggtcgggcc gccgccgaac
    16561 tgcggatcta cgccgcggag ctgagggagg ccgaccatgg ctgacgaact ccccggcttc
    16621 gacccacctc ccccgccgaa agacgccccg aagggcgagc ggctgcccca gcgggagcgc
    16681 gaccagctcg cgatcgccgc cggcatccac cccgcaacgc ggatgccgct tgccggcaac
    16741 ggcaagacgt gcggcgactg cgcggccctc cgcctcaagg ggcacccgag cgggaagcac
    16801 tggtggaagt gcggcacccc tgacccgaga tcgaagaacc accgcggcga cggccgcgac
    16861 atgaccaagc ggtggcctgc gtgcatcgct ttcaccgaga ggaccgaacc atgagcgatc
    16921 gccgcccgct tgtacctctt cccgtcgacc gacgatcgaa cctgaattgg gatctccgtt
    16981 cctcggaatt gtcggcgctc gacgacaacg ccagattcat catccgccgc atggtcgctg
    17041 agtcgtacca ggccgggtac gagcacggct ggggcgccgg ccaggacgac gcccacaccg
    17101 acaaggcgat cgcccgagac caagaggaga agttgtcatg aggatcctga cagttcgaaa
    17161 cccctgggcc gaggcgatca tctacgccgg caaggacgtc gagaaccgcg cgcggaacgt
    17221 cgcgggcgac taccgcggcc cggtggcgat ctattgcgcg aagagggtca acaccctcga
    17281 tgagtgggat gcattccgca gagtgcatcc ggcggcgcag cgtcgtctcg cacaggtggt
    17341 gcgtgagggg ctcccgctgt cgttcgggaa gatcatcggt gtcgtagacc tcgtggacgt
    17401 gcaccccgtg gcgaacccgc acttccatcc gatctgtcac gacctcaccg ggcccaacga
    17461 cctcgccgcg gccgccgcgc gaggggatgg cgcgtgctcg ccgtgggcgc acccagaggg
    17521 tcggtggcat cttgtgctcg cgaacccgcg cccgctggct gagccgatcc cgtaccgggg
    17581 tgcgctcggc ctccgcaacc tcgccgtcaa cgaccccatc acgaccgcgc ggatcctcgc
    17641 gcagatcgga gagaccgcgt gatctgcatc accaacacgg acctgcccgg catctgccgc
    17701 tgcacgatca ccggcgctca caaggtcacc tgccgggacc acgaaggctg ggcggagcgc
    17761 cccggcacct gccggggatg cctcccccgc ctcgccgagg tcggccacct gtgcaagggc
    17821 tgctacgagc gggtcgtagc agccgtcgcg gcatggccag cgttccggga gaagctggac
    17881 gccaccgagg gccgcgcggt cgcgtcattc ggcggcggcg ggtcggcgct cgggttcgcg
    17941 aacctcacgc tggtgttcct cgccgtcgac gaatgcgaac ggcacctcaa gtcgaggttc
    18001 ggcaccgtcg acaactgggt acagacggcg ccgtgcgcag ccgacgcgat ccagttctcg
    18061 cacgccgccg aacgcgcctg gaacgccctc cccgtcgaac cgaccgagga gcgcgccccg
    18121 gcaccgcacc gttgccacaa ctgcgggttc ctccccacct ggggcaacga cacccgcaag
    18181 gaacgcggcg cggacgtcgt cacctgcagg aactgcggca ccgaactcgc ccgcatccgg
    18241 cccgacatcg aacgatgggc cggatccgcg acctgcgagg accagctgca cgccgactgc
    18301 tccaacctcg cctgcacctg ccagtgccac gacctcggcg cccgctcacg tcccgccggc
    18361 gcgcaggcgc tctgggacgc cgaccagcac accatcagcc cgagcacctc gggccgcgcc
    18421 gactggagca tcagcgacgc actcaccatc acacccacca cgaaggagac acgagcagca
    18481 tgatccagat gggatgccac gcccgaggct cgaatgcgag ccgctctacg tgctgccgcc
    18541 gcagtgaagg gaggaggacg tggctgaacg ctcagccacg ctcgtctggc gactggctcg
    18601 ggatgtggtt gggtttcaaa gaccgcgccc gggtttcgtc tccggttctt gcggtcgacg
    18661 cgtccgaacg gccggctggc cgcggaccac gtcctccgtc gtccgcccag tgctcggacg
    18721 cgtctcggtc cccgtcatca gaagacaccg cgtcagcttc ttgctcgcgc tcggcgtcgt
    18781 tgtcccctgc tatgtcacgc accttcctcg ccgtggtggc ggcggcgcct tcgacagcct
    18841 ccgtgacgtc gttgaccttc ttctcaatgg catccaccac ccgttgcgtg agatgggagg
    18901 cgccctcgga cgccgacttg atgtggtctc caacggacat ggcgtgctcc ttcttcgtta
    18961 cccgccgaaa gcaggcgggc gtactcaggg ccgttaccgg tccttgcggc tgcggttcct
    19021 cacgaacccg aagatcacgg ttccgacgaa caggatcact ccgatgatcg cgagccagag
    19081 gaggccctcg atggcgaatc cggtgaccgc caggacggcc cacacgacaa gcaggataac
    19141 gaggacggtc cacatgttgc tcacgctacg cgcatccacc accgatagat atgggttctc
    19201 gattcgccca ggccaggagg accaatgacg aagccgaagg agtgcagcac ccgcccctgg
    19261 cgccctatcg aggaaggaga gacgctgtga ggtcagggct ccgagtacgc cacccgctcg
    19321 acatcctcag agaacggatt cgtcggctga tgacgcaccc agccgatccc gtcccagaac
    19381 cgccgcgaca cctcatcgtc attcaacgca gtcagacgcg gcgctgggta cagcttgcgg
    19441 atggcctgca acgtcgctct gccgatgccc tgccgccgcg ccgacaccgc aacttcgaac
    19501 gccagaatgt cgagctgccc gtgtggcatc ggtcccagcg gtgtatcgtc cagggggcgc
    19561 gcatccagga cgaacttgca gcgggccacc tcgacaccgt cgcggcgggc ggagatccaa
    19621 tggtcgctgt cgtcgactcg atgctcctca tcccaccacg gcgcaggaaa ctccgacgag
    19681 tgggcgaata cccgccagta gcggcccttc ggtcgcggac ggagctcaac gagcgtcagg
    19741 gcttcaacgt ccatacaccg acggtagcgg aaggacagcc agatgaagga gtgatcaccg
    19801 tcagggaagc cgccgctacg atccgctctc gaggtgctga gacatgcgct ccatgacgtt
    19861 ccgcatctga gcctggttca actggtgctc aagctcgttc cctggccaat ccttcagcgc
    19921 cgcgacaacg attcgtccgg ctcgcatcgt ctccactggc cgtccaagtc gtcgtgcggc
    19981 cgcgcgcgca acctttcgcc actccgccgc ttcgatgtca tcttcgatca cgcgctgagc
    20041 gccgccgttg aggcgcatct cgcgcatgat ttcctcttcg gattccatgg ctccaagcta
    20101 ctggcgccca cgcgcgcgtt ccagagcaag ctgcaagccc cgcggtgaca gcccagcgat
    20161 cttctgcatc tgcacccacg tgagccgccg ctgctcagcc atcgcgagta gctgccggtc
    20221 acggtactcg atgaggttcg ctcgccgagc ttcgacggct tcgagcttcg cgaccgtcgc
    20281 cttgagctcg gcgagttcct catccacccg cccactatac ccagtagcac tatagccggt
    20341 atagtgtaag aagatcggaa cggaggcagc gatgagcttc gagcggtaca gatgccctgc
    20401 gtgcgaccac ctatctgtga tcttcgatga ccagcccttc gcctgtcaga atccgacctg
    20461 cggcatcgac gccgctgtgc tccgcgaacc gcccgctgag ggacagacgc gcgagcagtt
    20521 catgcgagag cacgctctcg tctactacct cgcgctcggc aaccgagtga agatcggcac
    20581 caccaccgat ctccccagca ggatgaaagc cctcccccac gaggaactcc tcgcgttcga
    20641 gttcggcagc tacgacgtcg agcggacacg ccacaaccag ttcgcccatg cacgggctgc
    20701 tggcgagtgg ttcgaccgcg ccgacgccga gctcactgcc tggatctccg cactgcgggc
    20761 cgaccttgaa gacccgtcgg acgtgccccg atcagcctct cgtgcggtga agcggcgcat
    20821 cctgggattg gagcccgtgt gatgcttcgc atgaaaccct ggctcaccac agccgaagcc
    20881 gccgccttgt tcggccgatc caagcgcacc atccgactct gggtgcacga cggccgcaac
    20941 ccgaagaacc cgacgctccg catccgcacc gagaccaccg gccgtcgaac cctgctgaac
    21001 agcgaagacc tccgccgagt ggccgggttc aaggacgcgt acctcgacgc gccgacattc
    21061 ggacgtcaga gagtttgaat gttgccaatg ttgccgcgtt agtgtgtaag agtcggcata
    21121 ttgccgcgag cgaaagcccc cggaacgatc cctgacctgc ggtcacatcg ccgggggctt
    21181 cgtcacgaca cccagcccga caccggtgcc atgagaatgg tcgggatgcg gttggaggaa
    21241 ccgtcgtcct cgacctcacc gccattcggc tggctcatga gatggtccat ctcgtaggcg
    21301 agcttgagca gctgttcagt gaaactagcg acgtcggccg cctccccctc ggtaatcgcc
    21361 ggcagttcat gcgtcgcgga gttcccgacc ttgcggatgc gatccaccca aggcttcatg
    21421 cgccgagtga tcagaccctc cgattcgagg taggcgattg ccaccgtgaa gtcaggggca
    21481 cgacctcgtt cgttcttctc gggaaggcca aacccgacgg cgacatgcaa gaggatcttg
    21541 cgcgccatca gcaccgcgct cgtgtttgcg ccaacgccca gacacgagcg cacctcatcc
    21601 catgccgccg ccacctcttc gggtagaccc tgaggctctg ccagcggctt catgctcgga
    21661 tacaccgtcc ggtccacctc gaccagccct cgaccacagt tggaacagcg gagccagaac
    21721 acacgaccgc cggtcgtctg agcgatgacc agagcttgat tgcgtccgca catcggacac
    21781 gtcgacattc tgaacgcctc ggagctgtcg gtcttgatgt acccgtacat ccgctcagtg
    21841 accttcacgt caaggtgcat gtctcccata cggcgactct agccaacccc aacgacgctg
    21901 ctgatactgg gcacgaggcc ctgaagcgga ggaccacatg ccacgcatca ccgacgcgca
    21961 acgcgccgaa gtcctccgcc tccacaccgc cggaaaatcc cgtaacgaca tcgcccgctc
    22021 agtcggcatc agcgccggat ccgtcaccaa catctgcaag accgcgggcg tcggctttga
    22081 ccggtcagcg acgaaacacg cgactgaggc gcggcaggtg gacctggccg cagcgcggat
    22141 ccgactggcg gagaagatgc tgcaccggtc ggagcggatg ctcgatgatc tggacggttc
    22201 gtacctggtg tacaacttcg gcggcaagga caacacgtac gaggagcacc gccttgagcg
    22261 gcctccggtg gaggtgcagc ggaatgccgt ggtgaccgcg gggatcacgt tcgacaagtt
    22321 gacgcggatc gtggagcgcg agtcgggtgg cacggagtcg gcgctgggtc tgctggatca
    22381 tgcggcggcg gcgttcagtg cggcggcgga tgcgctgcgg gatgagggct caaccgatgc
    22441 tggatctgag tagcctcacc aggcacctgt cgcgggcgca gatcctgtcg gtggtggact
    22501 cggcgaagcg gtcgctggcg ctctggtacg ggtcggtgtc ggcggggaag acgatcgcgt
    22561 cgctgatcgc gttcctcctt gccgtgcgcg tcgcaccgaa gtcgggactg atcgtgatcg
    22621 tcgggcgaac cttgcagacc gtctatcaga acgtgatcgt gccgctgcag gatccgctgc
    22681 tgttcgggac tgtcatcgcg tcccaggtgg tctacacgcc cggcgcgacg aaggcgatca
    22741 tcctgggccg tgaggtcatg atcgtcggtg cccacaacgt ggagtccgtc ggccggatcc
    22801 agggtgcaac gatcggtctc gcctatgtcg atgaggcgac gctgctgccc gaggttttct
    22861 ggaacatgct cgtcacgcgt ctccgtgtcc gcggcgcccg cctgctcgcg acgatgaacc
    22921 ccgcgtcccg caaccactgg ctgcgccgga actggctact gaaggcggat gccgtcgacc
    22981 tgatcgcgtt ccacttcacg atgctcgaca acccgtccct cgagcagggg tacatcgagc
    23041 ggatgaagcg ctcctacacg ggcgtgttct acgaccggtt catcaagggc gagtggacga
    23101 acgccgaggg tgcggtctac ccgatgtggg atccgaaccg gcaccagatc cgcttcgagg
    23161 acatgccgcc gatctggcgc gtcctcggca tcgggatcga cttcgggacc acgcacgcga
    23221 catccgggat catggccggc atcacccgcg aggagaagcc ccgcctggtc ctgatggacg
    23281 agtaccggta cgacccccgc gatcacgaca accgccggca ggcgccgtcg acgaccgcgg
    23341 ctcagctgaa ggcgtggatg ggcaaggacc acgaaccgcg cccgtcgtca ctggtccccg
    23401 agttccacat catcgacccc gcggcggctc acttccagga agagctgttc aaactcggcg
    23461 tgaccacatg gcacgctgac aacgacgtgc tcgccgggat cggcacgaca gcccgcctcc
    23521 tcgacaacga ccagatgatc gtcaccaacc ggtgcgccgg gtggctgtcc gaggtcaccg
    23581 agtacgcctg ggatgcgaag gcgaccgcga acggcaacga cgagcccgtc aaggagaacg
    23641 acgactcgct cgacgccggc cgttacgtgg tccactccac gcgccagtcg tacgagtacg
    23701 acatcgcggc gtgaggctac tcccgctcga cgggagactc ccccatgccg agacgatgcc
    23761 cgtgctcgca ttcgtaatcc gcgacctgcg gtgcgcgcgc ggtgcgacgc ccaggccgca
    23821 tccccgtcga tgtcaccagc acgggtgtga cttctccccc atctcgggga cagaccagtt
    23881 ccatgccgca gatcgtacgc atcgccaccg acacccgagg ggagtccagc atgccgcttc
    23941 ccgctgacgg atccatcttc ccgcccaagc aactcgcccc cggcttcgcg cgcatgcgtg
    24001 agcacggcgc ctggtacacg ggcgacgtcg acgagctccg cgccatctac ggtggggcgc
    24061 agtccgcgac gcaccagcgc ggcggccgtc cgatgcaggg cggtgtggcg gggttcttct
    24121 cccgcatgtg gtggggtcgc ccgttggcgt ccggggaaga gcgcacgtcg ctgcacatcc
    24181 cgatggcgtc gaacatcgcc acgctgtcca gcgatctgat caacgcagaa ccgcccacgt
    24241 tcggtgtcgc tggcgaccgg gacaagaccc agcgtgacgc gaccgagcat cgcctcgacg
    24301 tgatcatgaa cagcccgcag tccgccatga cgcagaacga agccggcgag ctcgaggcgg
    24361 ctctcggcgg cacctactgg gtggccgact gggacaccac gatcgcggac catgtcttct
    24421 accggtcggt ggacgccgac gcggcggtgc cgacgttccg cggcaaccag ctggcggagg
    24481 tatcgttctg gacccgcatc gatgacggca agaaggtcta ccgtcatatc gagcaccacc
    24541 agcccggcgc gatcatccac gccctgtacg tgtcggacga ctccgaccgg atcggcatcc
    24601 gtgtcccact gaccatgcgt ccggagaccg cccacctggc ggagcttccc ggcgccgtgc
    24661 acgacgacct gcagacggtc atccccacgt acatcgaccg cctcacggcc gtgtacgagc
    24721 cgaacatccg caagccccgg aagctgcgcc gcaaggggac gttcgccgct tggggtcggt
    24781 cggacttcga gggaaacgaa ccgctgctga acgcgttcga tgagacctgg tcttcgtgga
    24841 tgcgtgacct gaaggtcggc cgggcccgca tcttcgtccc cgaggcgatg atccagtcac
    24901 agggccgagg gcagggcggg ttcttcgacg cggagcgcga ggtgtacgcg ctgctgaacg
    24961 ccctcccgaa ccaagccgag ggcaaggcga tcgtcgctca gcagttcgag atccgtgtcg
    25021 agcagcacga gcgcaccgcg ttcaacctga agaaggagat cctgcaggcc actggctaca
    25081 gcctctcctc ctacggtgag cacacccagg aagcgcagat gacggccacc gaggtgcaag
    25141 accgtcgttc ggacaccgag cgcacccgcg acaagaagat ccgctacgcc accctcgcac
    25201 gaacgcgcat cgcgtcgatc tgcctcgaac tcgacgggat catcttccgc agcaggggcg
    25261 gccggcccgg ggtggacgtc gtcgcggagt tccccgcagt gtcgcagatc gaccccgaga
    25321 aggaagtccg gatcgtgtcc ctcctggatg ccgcatcggc gatctccacg gagacgaagg
    25381 tgcgccgcgc gaatcccgac tgggacgaca tccaggtggg caaggaggtc gcgaagatcc
    25441 agacagagaa ggggctcacg gctcccgatc cggctacctt ccgcggctga tcagtcggcg
    25501 tcgtagttca ggtcgtcatc cagcgtgacg ttgagcgagg ctccctcagg gaggctccgc
    25561 gagagcagct ggaagttgag gcgcttcaga ccgaccttca gtacctcgat cggcgagatg
    25621 ttgtggacga ggaagcgagt cacgggaatc gggccgctgt tctcgtgatg cgccgcaaga
    25681 gacgcatagt cggcgatgcc ggtcgcctcg cggtcgatgg cgatgacgtt cagccgcgcg
    25741 gtctcaagat cggcgcccca cacgtcgata ccaacgatcc agaaccggtc aggatcgagc
    25801 ccgatcttgt tggcgagctc aagagtccca ccgccgtgag tgtcggcggt gacgctcccg
    25861 tcaaagtcac ggtactgcgc gtatgccggg gcaaggatct cgtcgttctc aacagccatt
    25921 gcggctcctc tcgtcagatg gccctgagcg tatcggtggc caccgacatg tgaatcgcag
    25981 tgtcacatct tcccggcgcg atgccgggca ggccccgtga cggggcgtca caggaaggca
    26041 ggcccatggc ccgcgaacag atcacccaca accacattgt gtcccggccg ctgccgccgg
    26101 ccgtcgagta cccgcccggg catcccgggg agctgctcga agctgtcgtg caggtggagg
    26161 tgccgcgccg gaacgtccac gtccagtgga accgcacggg cactacggac gctgcggggc
    26221 tcgagacggg ctggctgcag ctcggtatcg acatcaccat tgccgagatc cgcgacctcc
    26281 tgaagtacgc cgaggaggaa gctcaggccg ccaagctcag cagcgcggcc ttcggcgact
    26341 acgacactga gcagcatcag gtgcgcgtct actcgtcgga gctgtctcgc cccgagatca
    26401 acaaggcgat caacgctctg cgtcgcggac gtgacgtcgc ctatgggaag gacgcctgat
    26461 gctcacgccg gccgtgcgtg cgcgcatctc ggcggaggcc gatcggatct tcgctgaggc
    26521 tctcacggtc tccccgcgcg aggttggcgc gctcacagca tccgtgccac ggccgccgtt
    26581 cgtcgcccgt cgccgatcgc tccgctgctg gctcggcttc catcagtgga agtacttcac
    26641 gctcgtgctg ttcggcgtga tcgacatggg cgagcgacgc cgcagctgcg agcgttgcgg
    26701 gaagtgggcc cgcgcatgac gctctacctc cccgccgagg gcgagaaggt cacagcgctg
    26761 ctcggcgccg agctcggcat ggtcaccggc gtgtacggga tcgtgcagtg gatcgacgac
    26821 agcggcgagc agtgcatcgg tgtcctgtcc gctcccggac agacgcaggt gaccacccaa
    26881 ggtctgctcg tcgtggcgaa tgccgtcgcg gacacggaac tgaccacctt cgtcatggat
    26941 tcgctcggca gcgccgatgg ggactgacga catccacgtc tcccctgtct gcgacctcat
    27001 cgagcacgac gtcgacggcg gcgactgccc gtgcggcccg accaccaagc ccgtcccgcg
    27061 tgacgacggc tcgatgggct gggtgatcct ccaccatgcg ctcgacggcc gcgagttcgc
    27121 cgagcacgac tacaccggcc ctgcgatgcc gcgggagtcg tagagcgtcc aggggccctc
    27181 ctccccggtt gcgggcacgc gtcgcccaag aagacgagaa gagcgcaccg agcgcgcgcc
    27241 aggtaactca accctgccgg ctggtatccg cggtgagcgg gccgagtcag cccgcgcgct
    27301 cccacaactc aacacgccag agccggatcc cccaggtccg tggctggtat ccgcatccgt
    27361 agctcagcgg tagagagagc cgttcccaac ggtttggtcg cgggttcgat ccccgccgga
    27421 tgctcacacc acatgcccgg aggggcgtca acgcggtccc gagaatcccg cgaaggctcc
    27481 cacgaaagca cttacctccg ccggggacaa cctgcgcccc tcccctactc gtccgtgcac
    27541 gcccggtgct gcacggctca tccccgaaca ccgtcccgga ggcggagaag gagaaccacc
    27601 atgtccgacg accagaccac caacgacgag acctcgaccg accagacgtc ggccgagact
    27661 gacgtgacga cggacacggc aaccgccgac accgccgacg catcaggcga gagcaccacg
    27721 gatgcgaccg accaggggtc gaccgcaccg gagacgttct caaaggagta cgtcgagaag
    27781 ctgcgccgtg aggctgcggg gtatcgggag aaggcgaaga ccgacgcggc agccgccgcc
    27841 gaagccgccc gttccgaagt cgcacagcag atcggcaagg ccctcgggct ggtccaggac
    27901 gacactcccc cggatcctgc cgcgctggtc gcgcaggccg agacggagcg caaccaggcc
    27961 atcgagcgtg agcagtcgct gaagcggcag ctcgcggtcc tgaccggatc cgacaagcac
    28021 gacgcgaaca ccgccgagct gctcgactcc aacgccttcg gctcgaagct caaggcgctc
    28081 gaccccgacg ccgacgactt cgcatccctg gtggacgcgc tgatcaagga aacggtcgac
    28141 gggaacccca ccaagttcaa gcgcgtccag ctggcgcagc ggactggcgg cggcgaccac
    28201 tccggcgagg gagcatccca gctctcccgc gaacagctcg ccgcgcttcc gccggcagag
    28261 cgtctcaagg cagcgaagga ggggcgactc gcgtccctcc tcaagaacta agaaatgagg
    28321 gcctgacatg gccggaattg ccaacttcat ccccgagctc tgggaggccg ccgtccaggt
    28381 gcccttcgag aaggcgctcg tgtacggtca ggagaccgtc gcgaatcgcg actacgaggg
    28441 cgagatccag cagcagggtg acacggtcaa cgtgaccacc atcgctgatc ccacgatccg
    28501 cacctacgac aagaacgccg acatcgagat cgaggacgtc tccgacggca acgtcaagat
    28561 ggtcatcgac cagggcgact actacgcctt ccgggtcaac gacatcgaca aggtgcaggc
    28621 tgccggcgac ttccagtcgc ccgcgctcgc tcgcgccgca ttcggtctcc gcgacaaggt
    28681 cgacaagtac atcgccagcc tcgcctacgc gggcgcggac gccgccaacc gggtcggccg
    28741 gctcgccgtg gtggacgccg agccggagta cgccggcacc ggccagacct cggcgtacgc
    28801 cgtgctggtg aagctgcgcg agaagctcga cgaccagtcg gtgccgatcg atggacgctt
    28861 cgtcgtcgtc aacccggcgt tcatctccgc gctgtcctac gacccgcgct tcacgaacgc
    28921 ctcggcgagc ggctccaccg agacgctccg caacggcttc gtgaagcagg tgcagggctt
    28981 cgacgtcctc gtgtcgaaca acgtccccac ggtcggcggt gccggagcgg acaaggacga
    29041 caaggtcatc gtcgccggcg tccccgccgc gtggtcgttc gccaaccagc tggccgagac
    29101 cgaggccatc cgccaggaga agcggttcgc gaacctcgtc cgcggactga acatctacgg
    29161 cgcgaaggtc ttcgagtcga agggcctggc caccgcgacc gtcacgttcg cggcccccgc
    29221 cgtctgagca actggtgggg cgaggtatct cgcctcgccc caccacctga tctgagaggt
    29281 tcatgatgga cgacaccacc gagaccccgg ccgagacgcc ggaacccgcg acgcccgatg
    29341 ccccgaacga cgagggcatc gcgagcgaga ccgaggacga caccaccgag accccggccg
    29401 aggtggtgcc gctcaccgag atcactctgc cccgacggat gaacgtggtt ctctccggcg
    29461 ttcggttccg tttcggctct gcgcgagcac ggatcacacc gctgcagcgc ctctacttga
    29521 cccggcgcgg cgcgcgcttc gaggaagtgg cagaggatcc ggaacctccg gtggagcctg
    29581 cggcgcctgc tgacacgccg atgaccgaag ctgaactcgc cgaggccgcc tcgcgcgaag
    29641 ctgcgcccga gtccagcgct cagccggtca accagagcac ggacgagact gcggctgatg
    29701 ccccggacga cgctcagacc gaagagagcg agacgccccc ggcagaggtc ccggtccccg
    29761 acgagacgtg gaccgacgaa gctctcaacg ccctcggcaa ggagcgcggc atcacgtggc
    29821 gctcaaacgc atcgaaggcc acgaaggtcg cgaagctcac cgaaggctga gggaagcgcg
    29881 cgcccaggct tgaccccgtg tcgcctgggc gcgcgcccga aggaggcacc gcatgccgca
    29941 gttcctcccc gaccccgaag tcacgctcga cgacctgatc gagcagctcg gcgcgggcct
    30001 cgctgcccgc tacgcgcagg tcgagctcgt gctcatcgaa gccgccgcac gacggatgcg
    30061 gaagatcctc gcgctcgagc aggccgggga ggaagcggcg gccctagccg ctcggcagcg
    30121 tgcgctccgc gacgccgagc ttgagcgcgc tgcggcgctc gcagagctgc gacgaatcgc
    30181 ccagcaggaa gtggcgcgga tccgcaagga gggtctggcg cagcggatcg tcgctgtcgc
    30241 atcgcaggct ggccaggccg ccgccgcggc acaactcgca ggtgtgcgcg gtcgtgcgcc
    30301 ccgtgcgatt gggacgaccg ccgcgaacgc ggtggcgtcg ctcaccctcg acctggaatc
    30361 gaagctggat gtgctgaatg agcgcatcac ccggttccct gacgacgtgt acaagcaggt
    30421 gatgtcgcag tactcgccca gagtggtcct cggcgcgcag accacgcgcg cggctcaggc
    30481 cgccgctgtg caggacttcc tctcccaggg catcggccac atcgactacc tccgcaagga
    30541 cggatccgtg cacatgcgga tgccgatcgg ctcctacgcg gagatggtcg cccggacctc
    30601 atcccagcga gcatgggaag acgccaccgt ctatcggctg cagcagtcgg gtgtgaacct
    30661 cggcacgatc gccggcggcg cggacgcgtg cgcgaagtgt gctccgtgga tcggcgccgt
    30721 ggtctccttc gacggcacca gcggcaccgt ggccgtgccc tccgcggtcg acgggacgat
    30781 gatcaccgtc gacgtgaagg gcagcatcgg cgacgcccgc gcggctggat ggggacaccc
    30841 gaactgccgg gaccgcgtgg tcatgtactc ccccgggctc agcaacccgc aggcagtgga
    30901 gcacgccccg gcggcggaga aggaacgcgc ggaacagcgc cgcctcgagc gcgagatccg
    30961 cgcggcgaag cgtgacgagg ccgccgcgat gaacgacacc gaccggaagc gcgctgagca
    31021 ggccgtccgc gacgcgcagg gcaacatgcg cgacttcatc aagcagaccg gccggcaccg
    31081 ccagtcgtac cgggagcagc tcgggttcgc tgacggcctt ggcggtccgc gccccgagta
    31141 ctccgctcgc ccgccgaaga tgccgccgac ggtccagttc cgcgcaggga agggcacgcc
    31201 agtgggcgac gcggcgcggc atgcggtcac gcagatccga tccgtgcata ccatcccgca
    31261 gacgatcgag ccggtctcga tctcgcggat gagcgagctg cagatgcgat tcagcccgac
    31321 gacgctcgga cggtacacgc gagcggactc cgcgctgatc ctgcgcccgg acgccgaggt
    31381 ggagttcacg gccgtgcacg agctgggcca ctggctcgac aacaagctgt tcggcaacga
    31441 ccgcatgtac ggttccgatc aggtcgccag tgacccgtgg cggcgctggc aggcggccgt
    31501 gttcgagtcg cggtcgtcgc aggcgctgcg caatgtgctc gccctgtcct ccgatccgcg
    31561 gatcacgccc gagctcacgg cgacggcaga gtacctactg gaccctcgcg agcagttcgc
    31621 ccgctcatac gcccagtgga ttgcgatgcg cgccgcggac cggacgttgc tggaccagct
    31681 cagcacgtac acgaccgcca cgcacccgat cttgcgggcc taccattggg cagacgacga
    31741 cttcgaggcg atcgggtcgg cgctcgatga catcttccgt gagagagggc tgctgaagtg
    31801 atcacgcccg cgaactacca cgacggcatc acgttcgagg aagccgtcga cgggtacctg
    31861 caagagggcc tcgaccgtgc gcgcgccgag gtgtacgcga ggatcctcac ctcaccaggt
    31921 gaccccgaac ttccgatcat ctgagccccc gccccgtgcg gggctttctc attcccgccc
    31981 cgccgtgccc tgcctacggc ggggcggggc cgtttggagg catcatgctg cgcatcaccc
    32041 acccgcaccc cgaagagggc cgtcaggtca cgttcggcgt cgagttcgtc gacggtgtcg
    32101 ccaccgtcga gagcctgcac ccggagcgtg ccctggcact cacccagcac gggcacacca
    32161 tcaccaagct gtcgaacccg cggcggaaga aggcgaagtg aacctccgca cctacgcgac
    32221 ccttggtgac tacaacgacg tggccgaggt cgagtggccc gcgacggacg tggacacgct
    32281 gaacaagcgc ctccgtgccg cgtcgatcga ggtcgacaag ctcaccagac tgtcggtgtt
    32341 cgctgccgac gacgacggct atccgaccga tccggacatc gcagacgcgt tcaccgaagc
    32401 ggcctgcgcg atcgtcgagt actggcagga gaccgacgac cctaccggcg cggacgcctt
    32461 cgctggcgca gtgaagatcg gctccgtgtc ccttggcacc acgtcttcct cgcaggactc
    32521 gatgtctgat gccgagaagc tgcgccgccg gatcggttcc cgggcgatcg acatcctgat
    32581 gaacgcgggg ctggtccccg tggccgtcgc ccacacgtag gaggcgccat gacccgcctg
    32641 cgcgcgaagc atctccccca ccgggtcacg atccagcagc tcgaaggtga aggcgcagag
    32701 ggcgacatct gggctgaccc ccggccggat cgtcctgcgt acgtggagca gaagacccga
    32761 ctggtcgtgg accgccgcgc gacgtccccc accgtgggcc aagaggtcac cgcgacgacg
    32821 tttgtcgtga tcttgcccga cgacgactgc cagccgcgct ctcgagtgac cgtctggtcg
    32881 ggcacgacgc gcgagcgcac cgccgaggtc atcgacagtg cgttctttga ctaccacggc
    32941 accccgtcac acgtggagct gtacctggaa tgaggtgatc ccgtgggcgt cagcgcatcc
    33001 gtcaacctca ccatgaactt cccgagcgac accgagctca accagcgcat catgcgcggt
    33061 gagaacctcg ccgccgagcg tctgctcgct ctctcggccg ccgaagcacc cttcgacctc
    33121 ggcacgctgg tcggatctgg ctccgtcgag aacgccacca gccccgagga cggcgcggcc
    33181 gtgacctacg acaccccgta cgcggcccgc ctgcatgagc accccgagtt caacttccag
    33241 aacggccgca agggcaagta cgtcgaggat ccggcgctgc agcacaagga cgagctcggc
    33301 gcgatcatcg cgaaggagat cgcagatggc tgatgcaccg gagatcatct tgaaccgcgc
    33361 gctcgcgcag cttctcaatg accatgacct cgccgtctac cgcccggagg gtgcgatcgg
    33421 agaactcggg atccgcaccg acggtgtcat gccaacgacc gtaagcgagt tcacgctgat
    33481 caccccgctc cggcccatcc ccgatggccg cgctgatctg atctaccgcg cgcagatcta
    33541 cacgcgccgg aaaggcactc tgttgacggt ccggacctgg gccgccgacc tgcgggccgt
    33601 gctcgatcag gccgagtaca cgccgaacat cctcggcatc tcgttcgcca ccgagttctc
    33661 cgtgctcgac ttcgaaccgg actcgcaggg ccgcgccgcg gttgcctgca cctatcactt
    33721 ccgcggacgc cgtccgtagg aaccacagcc ggaagcgccg gcaccacaac gaaaggaccg
    33781 gtcatggccg actccaccct gtatgacacc acggcgccca gcgcgggttc cctggtcctc
    33841 gcccaccagc gtctgatccg cctaaagctc ggcggcgtct tcatcaacat cacgggcgac
    33901 gtgaacaacc tcgccctgaa cccgacgaag atccccgtcc cccgcgaggt ctacggcacc
    33961 aagggccgtt cctccgagga catcatcgga tacaactacg ccccgtcgtt caacgtcgag
    34021 gtcgtccgcg atccggtgac caagcagatc gtcgccgcgc aggcgtggtt caaggatctc
    34081 gtcaaggcgg cgttctcgga gggcgccgcg aataagcgcg agttccagct gttcaccgac
    34141 gcgctcgacg aggacatgcc ggtcctgcag ggcacgttct ccgtcgcgta cagcgaggcg
    34201 aacaccggct acgccgacaa gggcgtcgtc accttcaccc tcgcgaacga cggcgtggtc
    34261 aaccagatca ccccgtcgcc tctcgccgga tccggcgtcc cgatcctgga gtccgcgacc
    34321 ccgacgctgc aggcgcccgg gaagatcatc aaggtccgcg gctacaagct tggcaacgcg
    34381 gtctccgcga ccgtcggcgg caaggcggta ctcgagctgc gcgtcatcga cgagtacacg
    34441 gtcgcgatcc tcatcccggc caccgtcacc ggctcctcgc ccatcatcgt gaccaacgct
    34501 gccggggcgt ccaactcgct gccctaccag gcagcctgat cgaagggatg gcagggatcc
    34561 catgatcaca gcagagaagc agggccggaa cctcgtcctc accatggagg gggtggatca
    34621 cccgttcgtg attcgccccc tccccgggca cgccggccgc cagatcaccg acacctatct
    34681 cgcaacgatg ggcggccgtc cggatgtcga cttgctcgcc gcgctgcaga tggccgtcga
    34741 cggcgcaatc ctcgacggag accgctgggc gcctcgcccg gagagcgagc aggagaactt
    34801 cacccgtatc ggtatggaac tctcccagga cgaggccgag cagatcctca tgcccgcgtt
    34861 cttctggcag acgatcctcg gcatggacgg cgttcgcgcc tacatcgaag gcggtgaggg
    34921 cctcgccggc accctaaaag catcgggggc gctgtccgtc cggttgggct tcttggtccc
    34981 gcggacatcg cccagttcgg attaggcgaa cccgacgaga acggcatcta tcccgagtac
    35041 ctctacccgc ctgggtggcg agagtcgcgc gagcaggcga ccgccgaacc aacgcgaggg
    35101 ctcacagctg ccgaactgtg gaccatcgcg cttcccgagc tgttcgggga gatcgaactc
    35161 gacctcgccc aacaccacct gatcaccgac ctcgaccgcg ctctcgacac gcacacgtgg
    35221 cacttcgtgc gcggcgccgt ggagcgactc atcgacattg acgcctcatg gctcggaagg
    35281 gcggtggctg atgttcgacg cgggcgcact cgtgttcaag ctccagacgg tcggggctca
    35341 ggtcttcaag caggatcagg ctgacgcgaa ggccgcggtc gagaagaccg gtcaggcgtc
    35401 caaggagtcc gcagacaagg tcacgggact cggcacggcg accgatgcca cgggcaagaa
    35461 ggcgaaggcc gcgaagggtc cgctcgacga gcaagcggca tcgaccaaga aggtgggtga
    35521 cgagtccgac aactcctcga agaaacagga caagcagaag tactccaccg agcagcagat
    35581 cgcagccgcg aaggaactgt cgaaggtcct gctgatcgcg ggcgccgccg cggcggcgat
    35641 ggtcaccctc gcggtggcga agaacaccga gttcgaccag gcgatgtcgc agacccgcgc
    35701 cgcgacgatg gcgacagccg ccgagcagaa gaagctcggc gaggctgccc tcgacgctgg
    35761 cgcggacacc gcgtacagcg cgtccgaggc tgccgccgcc gaggaggagc tcgcgaaggc
    35821 gggtctgtcg gtctcgaaca tcgtcggcgg ttcgctgaat ggcgcgctgg ctctcgcggc
    35881 cgccggacag ctgcaggtcg cacgctccgc ggagatcatg gcgaccacgc tcacccagtt
    35941 cggactcaca gctgacaagt ctgggcacgt cgcggacgtc ctcgccgctg gcgctggcaa
    36001 ggcgcagggc gaggtcgagg atctcgccct cgcgctcacc tacgtgggcc cgctggcgaa
    36061 gtccgccggg tggtcgctcg aggagacggg cggcacgctc gcctacttcg cctcccaggg
    36121 aatcctgggc gagaaggcag gcacctctct gcgcggtgtc ctcgcggccc tgcaggcccc
    36181 ctcaacgatc gcggcgaaga cgatggacca gtacggcctg tccgtctacg acgcgcacgg
    36241 gaagatgctg tccgcatcgc agatggcagc gcaattgcag cacgcattcg gtggcctcac
    36301 ggaccaggag cggaacgctg cgatggggcg catcttcggc aacgagtcgc tcaccgccgc
    36361 aaccctgctc tacaacggcg gcgcacaggc aatctcgaag tggaccgacg aggtcaacga
    36421 cagcgggttc gccgccgagc aggccgccat gcggcaggac aacctcgccg gcgacatcga
    36481 gaagctcggt ggcgcgttcg acaccgcgct gatccgcacc ggctccaacg ccaacgacgt
    36541 gctgcgcgcc atggtccaga acgtgaccat gctcgtcgac atgtacgggg acgcgccaga
    36601 gccggtgcag gcgctcgggc tcgtgctcgg cgtcgccact gcggccatcc tgctgttcgc
    36661 cggtggggct gtcggtgccc gggcgaagtt cgtcgagctc aaggcgacgc tcgacgctac
    36721 gaacgcgagc atgggtaaga ccgcgctcgt gggtggcgcg gcgggtcttg ctctcaccgg
    36781 aatcatcgcc gtcgtagggc tcctgatgag cgtgcaggcc gagggccgac agatcgccga
    36841 gtcgtatgcc gatgcgatcg atgcgagcac cggcaaggtc accaaggctg cacgggacat
    36901 cgccgtcgag aacctgtcca aggcgggagc ctggttcacg ttcaactggc agtcggcgta
    36961 tgacgccgct gacaagctcg gtctcagcct tgacaccgtc accgatgcag cgctcgggaa
    37021 caagaaggct ctcgccgagc tggacatcta cctccgtgcc gcggaggaag atcacgacgc
    37081 actgaaccag gtagtggaga agacaggtct cagccagcag gacgcgctgt tcgccgtcgg
    37141 tgccctcaag acgggcatcg atcagcagaa caatgccctg aaggagggca agaagctcca
    37201 cgatcagaag tccagggccg aggacgaagc caccgaggtc acgcagacag cgactgaggc
    37261 gtacctggac caggccggat cgatcgacaa gctccagaag caactgtccg atctgatcga
    37321 cacgatcaac aaggcgaacg gcgtcaacca ggacgcgatc acctcgaaca tcgactacca
    37381 ggagacgctc cgcacggtcg atgagcagat cgcgaagatc aagtccggcg cggacggcta
    37441 cgcgaggtcg ctcgacatca ccaccaaggc ggggtcggac aacaaggcca tgctggtgga
    37501 gctcgcgcag aaggcgtggg acgcggctga ggcgcagctg cagctggacg ggaacacgga
    37561 cggcttcacg tcccgcctcg aggcgtcccg gcagaagttg tacgacactg cgattgccat
    37621 gggcgcaacg gacgcggagg ccgcggcgct gcgtgacagc atcctcgcga tcccttctaa
    37681 gaaggagatc cagatgatcg cggacacggc gtccgcgcag aatcggatcg atcagttcgt
    37741 gcggctgaac aacggccgca ccgtgcgcgt gcacgtggcg gtcgacgggt cgcagtcgta
    37801 cagagttggc agcaagaccg tcgattcggg cttcgcgaac ggcgctttgg tgcagtacaa
    37861 cgcaaacggc aacttcaccc cgtccgagca ccacgtggcg cagatgctcc gcggcggggc
    37921 gatccgcgtc tggaacgaac ccgagactca gggtgagacg tacgtcccgc atgcaatgtc
    37981 gaagcgctcc cgttccgagg cgatcatggc cgcgaccgcg gagctcttcg gtggcgtcta
    38041 catcccggcc ggcgcgaagg cgttcgcgga cggcggcggg gtgtcggtcg gcccgagcgg
    38101 cggcgcggac gtagtcgccc tgctacgcat gatcgcggcg aagctcgacc ggccgaacct
    38161 gtcgatcgtg aatccggtga gtcgtgatgc gaagtccgat gcgtgggagg ctgcgcagat
    38221 cgtggggttg tgaatgttca gcatcaatgg catcgcgctg gacaacgccc cgctcgggtg
    38281 ggtgctgcgc gcaccgacgt ccctgctgtt cgaaatgtca tgggagcgcc cgtcgctacg
    38341 cattccggga acggatggcg tggtgtcggg cctccccgca gaccgtaacc cggtcgtggt
    38401 cccgttcatg gtgcagacgc cgaggctgaa ccttgagtcg ctgctcgcgc tcgtcggcag
    38461 caacggcgtc gtgacccgca cgaccgcgcc gacccgtcag gccgtgtacg agacgatctc
    38521 cgtgcgccaa gttgggtacg ggccggcaga ggcgatcgtc gatgtgacgt tccagcttcg
    38581 tttcccggaa cccgcatggc gagacatgac cccggtggag aatacctacc cggtcaccgc
    38641 ggcgatccac gacaacctgt cggtgctgcc cgggctgtcg gcccctgtcg gggatgcact
    38701 gatccggttc gtcggcccgt tcacgtacgc gaacgttgag tcgacgtcgc tggccggcag
    38761 gtcgttcgcg accctcactc gcactgtcct ggccgggcag tgtgtcgtgt tcgacgccgc
    38821 gacgagcgtg tgggtgcaga acgcgttccc gacccagtgg tcgctcgtcg gaacggacgt
    38881 caccggggac tcgttcctcg gcgggccccg cgatcggttc gagatcggac cgtccttcac
    38941 cgatccgacg gtgcgcacgg gccgggtgaa ggtcaacgtc aatggttacg ggaccgggac
    39001 gagcttcggt gtgcgcggga gacgcgcata cctcgacgtg taggagacct tcatgctgcg
    39061 tgttcgcctg atgaacgacg acctgacccg ggggaagtat ctccgggcgg cgaaggtgga
    39121 ggtcacgcag gtgtcgtcgg cgtcggcgac gatcgcggtg accgtgtcgg agcgggtcgc
    39181 ggggcgcatg gacagcccgc agttggtgtc ggtcgaggtc gctggtgcgc agatccgcac
    39241 gaacctgttc atgctcgacg aggacgattc ggacgacgcc gaccagacga agatggtcac
    39301 cctgcgaggg gtcgactatg tgacctggat gctgcagcgc acgctgctgc actggtcggc
    39361 gtcggcgaag aacggtgaac gccagtgggt gcagttcccg tccgggagca ccggctccgc
    39421 gacccctggg tacacgctga acgggatgct cgtcgagtcg aagaaccgcg ggtggggcgc
    39481 gaacgtgacg tgggacttca cggggctgaa ggattccgcc ggtgtcgcgt ggtccctcgc
    39541 gggcgatgac ctgtggtgga gcgcgtggac gctcctgacc ccgctgtcga aggtcatcga
    39601 gatgctggct gagcagggct tctgcgagtg gtggtctgag ggtgggaagc tccgcctgtt
    39661 ccgccctggc accgccgatg accacacgag catcgtgctc ggcggggccg ggttcaagtc
    39721 ccgcccggtg aagtcgtcgt tcaagaacgt gatcacgaac ttcaccgtcg tgccggagaa
    39781 ggcccgcaac tggctgtacc tgccgaaccc gggcgcgagc acgcgcttcg gccgcctcga
    39841 ggcctcgatc tcccagtcgg gggtcgctga ccacgtcacg gcgacacgcc tcgcccagcc
    39901 ggcgttgacg gcggcgcgcg ccgcgcagca gcagttctcc ttcgactggg atctggtcga
    39961 aggcatgccc gtccccggcg tcgacttcaa catcggcgac ctggtgacca cgaacacccg
    40021 cgccggccgc gcggtgcagc gagtggtggg catccagttc acccgcgaat catcatccac
    40081 cagcgtgcgc gccatcaccg gtgaccggct gctgaccgac gcgctcaagc gcgctcggaa
    40141 gagcgcctcc gcggtcatcg gcgacatcat cggcggctcg ggcaacgggc tgcccacgtc
    40201 gaagggcccg acgttcccgg tccctgcggc gccgacgggc ctgcatgtgg agacggatgc
    40261 atctcagtgg ggtgcggatg gctccgcggt cacgtcggtg acgctcgcgt gggacgcggt
    40321 ctctcaggct gaggatgggt ccggtgtgga cgtgtcggcc tacgaggtcg gcatccgcac
    40381 ggacgggcag cgcggcgtcg tcgcgacgac ggacgcgctg gaatggaccg cgaccggctg
    40441 gcccgtcggc gtgaccgtgc aggtgtccgt gcgtgcggtc gcgccgaacg gtgaccgggg
    40501 tgcatggtcg gccgagatcc ccgtgacgcc caccgacccg gcccggcccg tgaaggtgac
    40561 cgggctgacc gtcacctcac agcagggctc ctggcgtgcg gatgggaccg cgcagaccgc
    40621 ggtctctctc gcatgggatg cggtatcggt ggataccggc gggcacgtga tccctgtcac
    40681 ctacgaggtg caatcccgcc ttcccgtgca gtcggatgcc aacacggatc agacgacggt
    40741 caccatcacc gggtgggagc ccgggaagac ccggtacgtg aaggtccggg ccgtgtccgg
    40801 aaacgggaag ggcgactggt ccgacgagct cgccgtgcct gtcgcggttc ctgccagcat
    40861 cgtcccgaag cctcccgcga acctgcagct ggtcgtgaac atcggcagct ggggtgaggg
    40921 gggcctcgcg atcggtgccg tgtatgtcca gtgggatcgc gtcaccgagt cggtggacgg
    40981 cgcgccggtc accatcgtcg agtacgaggc gctggatgtg agctcgtacg aacggctgca
    41041 ggtcttcgat atcgacccgg cagacttgcc gtacgcgctc ggtgtcttcc cgtcgaagag
    41101 catggcgcag gtcgccgtgc gggccctcac cagcacgggc gtttggtcgg acttcgccgt
    41161 cctggacccg cccgttgagg tggcctcccc gacactctcg gtgatctccc cttcagcgct
    41221 ggtcacgtcg accgggctgg gtggggcatc gggccggtgg gatgggaagc ttgccggcgg
    41281 cgtggacgcg tccacagtcg cggggttcgc gtcggtgcag gtcgagacgg cgacggctgc
    41341 ggcaggcccg tggtcccctg cgggctcgcg actggcccgc gcgggcacgg tctcggtgca
    41401 gtcgcagacc ggcgcgacgg tctacttccg ggcgacggcg tacgacacgc tggggcgggc
    41461 catggggacg tccacggtgt cgtcggtggt cgtgcagggc gtgagcctgg acgacatccc
    41521 tggcctcgag ggtgacctgg acgacatccg gttcaccgcg gatggcaaga accgtgtcta
    41581 catgtccgca acggagccgg tcggtgatac gccgacggtc gtgaagcgcc gcaacgcggc
    41641 actgaacccg caacaggtcg ccagcgggtc ggaccgcgag gctcagccac ggtactcctg
    41701 gtcgaggtcg tggacgacgg gaatcatgga tcaccccctc gggctcgata ccgcgctgcg
    41761 cgtgaccctg acggctgcgt cgcagacggg cgcggggcgg ggcttcgact ggtacggcaa
    41821 cgcggacacg gccccggcgt cgtcgggcgt gtggctcagc gcggtcgagg tcaccccggg
    41881 gcagacaatg catgcgaccg tctgggcgcg cacgaaccgc aacggcctgc cgttcgcgtt
    41941 cggtgtccgg ttcttcgacc acgtcaccct gacatggaag agcactctgg cgtactcgcc
    42001 ggtagtcgtc tcgatcgcca acggctgggc gatgctcgag ctttccgcgg tcgttcccgc
    42061 cggcgcgact cgtgccacct tccgtggcgt gtcgaaccga gactcggtcg cgttcgtcca
    42121 gaacgactgg atggaacaga ccgggatcat gatcggcccc ggcacctacc tggatgggcg
    42181 caccgctccg aagcctgggc tcgggtacaa gtgggagggg acgccggatg cctccgcatc
    42241 ggtggaggtg gagtcggcgt tcgctcccgg agacctgtgg ttgcagctgg acccgacggg
    42301 cgcatcgatc atctcgatcc tcgtctggaa cgggactgca tgggcgccgc aggtgctcta
    42361 cgcggacagc atcatcgctg cggaatcgat cacctcccag ctcgtccggg cgggcgcgat
    42421 cgaggtgaac cacgtgtcgc cgtcgttcgg ggactcgctg aacctgcagg gcaacggttc
    42481 gatcaacctc ctggctggtc gggcggatgc cgcggcagaa cagctcacct cccagcaggc
    42541 ggcgcttgac cagcagtcgg cgcagctcgc ggcggcgcag gcggcggcag atgccgcgca
    42601 ggctggg
//
LOCUS       DATMSX010000572         5170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_132560,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000572 DATMSX010000000
VERSION     DATMSX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132560"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VN041_18470"
     CDS             complement(<1..850)
                     /locus_tag="VN041_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31071.1"
                     /translation="MSVPVVVMRSVQAVQRTSVDAAFESVSADVGKVAHAEGFGGPLG
                     EAINNNLTKLIADLDEVSAYHKELGKLVGLADQAMHETATGVAGLPAPELTSDQQDTV
                     KTASETNSPVQVSPGVMMTSAEAKQYYLDQAEAEQEEAARKMAVALDARLQELIDQMP
                     VSKYDPPKPSTEDPTGGDGTGDGSSNYGGVSGGTDGTGSGTGSGGGGQYIGPGGTGGT
                     DNPRPPHYVVDPRPPHYYVDPPYRPPYDPPYNPNDPPNVDGGGDGTVPGGPGGPGGPR
                     VVSPGGP"
     gene            complement(908..1261)
                     /locus_tag="VN041_18475"
     CDS             complement(908..1261)
                     /locus_tag="VN041_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31072.1"
                     /translation="MSMDKDVVEYQVKRVVDSAEQIGKAKEALASLQDGQTPVLWTAD
                     GKCVDLTKVLKERYHSSELAVNDILKRLIEIRDNLKKAIDETDQLDADQKQGYQVILA
                     NALGSVTKPSGPIAV"
     gene            complement(1391..2206)
                     /locus_tag="VN041_18480"
     CDS             complement(1391..2206)
                     /locus_tag="VN041_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWU31073.1"
                     /translation="MSTPAGWYDDGSGRQRWWDGSQWTDNYAPAGDSAPVDGSAASEG
                     DSAAPAAGSDAYAAPAYAPVAPASTAPTQAPTLGFIGLGLAVLGTILACIPSFVTFGI
                     GSFVLLAAFVVSLIAVFKRGTVKWPSIVGIALSIIGGIIGAIVLTITIATALANIPVP
                     DLPTAPSSSPADEPSDPPATGDSEGRPSPEAIGDGWKISLNAGGFHQYDDNPEFFACI
                     GEYLYDSDLSDEVLSDIAQGVDNTTGAERDHAIQVGQDATYECDPSLPRPTDQ"
     gene            complement(2304..3113)
                     /locus_tag="VN041_18485"
     CDS             complement(2304..3113)
                     /locus_tag="VN041_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HWU31074.1"
                     /translation="MSTPAGWYDDGSGRQRWWDGVQWTDQYAPGSDAGTGGASPMATP
                     YVSAQYAAPRYAAPQYAAPQYAAPGYGPALTPVAPRQPPTLGFVGLGLAALGTALACT
                     PILVTFVLGALVLIAAFVVSLVAVFRKNTAKWPSIVGICLSIIGGIVGAVVFTVVLLV
                     TLTTAIIESVPSAFPTSAVSEEPSTDTGVGRPSPEEIGRGVQTIFHDRGDTSQDGDPE
                     FFPCMGQFFYDSDLSDRALQTMAEAGDITGPERESAEELTRAGKVACGGQQ"
     gene            complement(3166..3924)
                     /locus_tag="VN041_18490"
     CDS             complement(3166..3924)
                     /locus_tag="VN041_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31075.1"
                     /translation="MTDLSQPGGAEATQGGALMLDFGGRRERIPAGATFVIGRGTDLS
                     IDDNPYVHRRFLEIAQRDGMWWLTNAGSGLTASVASADGLAQAWLAPGATMPLVFGAT
                     TVMFTAGETTYELSLAAEAPYYQVSASWNPGAGIDGVTEMLSPVQRILLTALAEPMLR
                     PDAEGAVQLPSMEQVAVRLGWSLEKLERRVATLCSKFARMGIQGLERNAQGELLASAR
                     SRIVEHAVGARIVTAEDLELLDAFVAQDAVGATL"
     gene            complement(3927..4718)
                     /locus_tag="VN041_18495"
     CDS             complement(3927..4718)
                     /locus_tag="VN041_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31076.1"
                     /translation="MTRNTGSGGRSSSRRHADGNDTTAQNAEKSKSDFDRFTGFIDTV
                     VDIINGGISGLLDLVLPDGIMDRINAWLDKMFAKVNEAIDKAAQVFAYVGSPSTLRST
                     GLDWVQSVGTPAQQCTSTLLPTALPTTGNWEGKAYNAYRLRVDQQKPAADDVLSKCQM
                     INTQLDSFAGAIENFWIGFLGAVGSLVVGVIATAAELAGVVTAPAAIVTIVGAVGLFI
                     TFLVDAISAITSASQATSSFVVNVTNALASTSAFPGGAWPRHTAA"
     gene            complement(4752..>5170)
                     /locus_tag="VN041_18500"
     CDS             complement(4752..>5170)
                     /locus_tag="VN041_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31077.1"
                     /translation="STGTDGSTGTDGSTGTDGSTGTDGSTGTDGSTGTDGSTGTDGST
                     GTDGSTTPVGNVDGGPEPVPGPDGRIVGEPLPDVRPGIDDLVGDSRPDVRPGFNVDGT
                     IGTPAAESHLSASIDAESRPLPADPAAPAWSSRLGN"
BASE COUNT          826 a         1792 c         1690 g          862 t
ORIGIN      
        1 gcgggccgcc ggggctgacg actcgcgggc cgccggggcc accagggcca ccgggaaccg
       61 tcccgtcgcc gccaccgtcg acgttcggcg ggtcattggg gttgtagggc gggtcgtacg
      121 gcgggcgata tgggggatcc acgtaatagt gcggcggacg cgggtcgacc acgtagtgcg
      181 gcggacgcgg gttgtcggtg ccaccggtcc cacccgggcc gatgtactga ccgccaccac
      241 cggacccggt tcccgagccc gtgccgtccg tgccaccgct cacgccaccg tagttcgagg
      301 agccgtcgcc ggtgccgtcg ccgcccgtag ggtcctcagt cgagggcttg ggcgggtcgt
      361 acttcgacac cggcatctgg tcgatgagct cctggagccg cgcgtcgagt gcgaccgcca
      421 tcttgcgggc ggcctcctcc tgctccgcct ccgcctgatc caggtagtac tgcttcgcct
      481 ccgcggaggt catcatgacg ccgggcgaga cctgaacggg gctgttggtc tcactcgccg
      541 tcttgacggt gtcctgttgg tcagacgtca actccggcgc gggcagaccc gccactccgg
      601 tagcggtctc gtgcatcgcc tggtccgcaa ggccgaccag cttgcccagt tctttgtgat
      661 aggccgagac ctcgtcgaga tcggcgatca gcttggtgag attgttgttg atcgcctcgc
      721 cgagcgggcc gccgaaaccc tccgcgtgcg cgaccttgcc cacatcggcg gacacggatt
      781 cgaacgccgc atcgaccgac gtcctctgca cggcctggac gctccgcatg acgacgaccg
      841 ggacgctcat cctgttgagc tggagcctac cgaggcgctc tacgttctta cccacgatgt
      901 tctccgttca gaccgcgatg gggccgctgg gcttcgtgac cgagccgagg gcgttggcga
      961 ggatcacctg gtatccctgc ttctggtcgg catccagctg atcggtctcg tcgatggcct
     1021 tcttgaggtt gtcgcggatc tcgatgagtc gcttcaggat gtcgttgacc gcaagctccg
     1081 acgagtggta ccgctccttg aggaccttcg tgagatcgac gcacttgcca tcggcggtcc
     1141 agagcacggg cgtctgcccg tcctgaaggc tcgccagagc ctccttcgcc ttgccgatct
     1201 gctccgcgct gtccaccaca cgcttgacct gatactcgac cacgtctttg tccatcgaca
     1261 tggatgaatc cagtggtgtc accttgatga ccacgacacg cccccctctg ctgttgctgt
     1321 ccgcgacgat actaacccgg tgtcgaggtg tcttccatgg gtagcattac ccaccctcgg
     1381 caccgagcgg tcactgatcc gtcggtctcg gaagggaggg atcgcactcg taggtcgcgt
     1441 cctgtccgac ctgaatggca tgatcccgct ccgcgccggt cgtgttgtcg acgccctgag
     1501 cgatgtcgct cagcacctcg tcagacagat ccgagtcata gaggtactcg ccgatgcatg
     1561 cgaagaactc ggggttgtcg tcgtactggt ggaagccgcc cgcattcagg ctgatcttcc
     1621 acccgtcgcc gatggcttcg ggcgatggcc gaccctcgct gtcgcctgtc gcaggcgggt
     1681 cggagggttc gtccgccgga gaggaggatg gcgccgtcgg gaggtcggga accgggatgt
     1741 tcgcgagggc ggtcgcgatc gtgatcgtca gcacgatcgc cccgatgatt cctccgatga
     1801 tcgacagcgc gatacccacg atcgagggcc acttcacggt gcctctcttg aagaccgcga
     1861 tcaaggacac cacgaaagcc gccagcagca cgaaggaccc gatcccgaac gtcacgaagc
     1921 tcgggatgca cgccaggatc gtgccgagca cggcgagccc caggccgatg aaaccgagcg
     1981 tcggtgcctg cgtcggcgcc gtggatgccg gtgcgacggg cgcgtaagcc ggagcggcgt
     2041 acgcgtcgga acccgccgcg ggagcagcgg agtcaccctc cgatgctgcg gagccatcca
     2101 ccggggcact gtcacccgca ggggcgtagt tgtccgtcca ctgcgaaccg tcccaccagc
     2161 gctgccgccc ggaaccgtcg tcataccaac ccgccggagt gctcatccac atcctcctcg
     2221 tcgaaaccat ccgcacgcct cagacgcaca gcccccatga cgcgaaagcg tccctacgtg
     2281 tatatcaggc gaactcgaat ccctcactgc tgcccgccgc aggcgacctt ccccgcgcgg
     2341 gtcagctctt cggcgctctc acgctcgggc cccgtgatgt cgccggcttc agccatggtc
     2401 tgcagggcgc gatccgacag atccgaatcg tagaagaact gccccatgca ggggaagaac
     2461 tcagggtcgc cgtcctggct cgtgtcgccg cggtcgtgga agatcgtctg gacgccccgc
     2521 ccgatctcct ccggcgacgg ccttcccacg ccggtgtccg tcgaaggctc ctcgctcaca
     2581 gcagacgtgg ggaacgctga cggcacggac tcgatgatgg ctgtcgtgag tgtgaccagg
     2641 agtacgaccg tgaagaccac ggcaccgacg atccctccga tgatcgacag gcagatgccc
     2701 acgatcgacg gccacttcgc ggtgttcttc ctgaagaccg cgacgagcga gaccacgaat
     2761 gcggcgatca gcacgagcgc gccgagcacg aaggtgacca ggatgggggt gcacgccagc
     2821 gcagtgccga gggcagccag tcccagaccg acgaaaccga gcgtcggcgg ctgtcttggc
     2881 gcgaccggtg tcagagccgg accatatccc ggcgccgcat actgaggcgc cgcatactga
     2941 ggcgccgcat accggggcgc cgcgtactgc gctgacacat acggcgtcgc catcggcgat
     3001 gcaccacctg tgccggcatc cgagcccggc gcatactggt cggtccactg aacgccgtcc
     3061 caccagcgct gccggccgga gccgtcgtcg taccagcctg cgggggtgct catccggacc
     3121 ttcctcgccg tcgtgttgaa cgcccggccc cctcgatgct caggctcaga gcgtcgctcc
     3181 gacggcatcc tgagccacga acgcgtcgag cagttcgagg tcctcggctg tgacgatccg
     3241 cgcgccgacc gcgtgctcga cgatgcgcga gcgtgcggag gcgagcagct cgccctgcgc
     3301 gttgcgctcc aggccctgga tgcccatccg cgcgaacttg ctgcacagcg tggcgacccg
     3361 gcgctccagc ttctcgagcg accatccgag ccggaccgcg acctgctcca tcgacggcag
     3421 ctgcacggca ccctcggcat ccgggcgcag catcggctcg gcgagcgctg tgagcaggat
     3481 ccgctgcacg ggcgagagca tctcggtgac cccgtcgatc cccgcaccgg ggttccacga
     3541 ggcggagacc tggtagtacg gagcctcagc cgcgagcgac agctcgtacg tcgtctcacc
     3601 ggcggtgaac atcaccgtcg tcgcgccgaa aaccagaggc atcgtcgcac ccggcgcgag
     3661 ccaggcctgc gccagtccgt cggccgaggc gaccgacgcg gtcagccccg acccagcgtt
     3721 cgtgagccac cacatgccgt cgcgctgcgc gatctcgagg aaccgccggt gcacgtacgg
     3781 gttgtcgtcg atcgacaggt ccgttccgcg cccgatcacg aacgtggccc ctgcggggat
     3841 gcgctcgcgg cgcccgccga agtcgagcat cagggcgccg ccctgtgtcg cctcggcgcc
     3901 ccccggctgc gagagatccg tcatgctcac gccgccgtgt gacgcggcca ggccccaccg
     3961 gggaatgcgc tcgtcgacgc cagggcgttc gtgacgttca ccacgaacga cgacgtggcc
     4021 tggctggccg acgtgatggc gctgatggcg tccaccagga aggtgatgaa caggcctacc
     4081 gcacccacga tcgtcacgat cgcggccggc gcggtcacga caccggcaag ctcggcggcg
     4141 gtggcgatca cgccgacgac cagagagccg acagcaccga ggaagccgat ccagaagttc
     4201 tcgatcgcgc cggcgaagct gtcgagctgg gtgttgatca tctggcactt cgagagcacg
     4261 tcgtccgcgg ccggcttctg ctggtcgaca cggagccggt aggcgttgta ggccttgccc
     4321 tcccagttcc cggtcgtcgg cagtgcggtc ggcagcagag tggaggtgca ctgctgcgcc
     4381 ggcgtgccga cgctctgcac ccagtccagc cccgtgctgc gcaacgtgga cgggcttccg
     4441 acgtaggcga agacctgcgc ggccttgtcg atcgcctcat tcaccttggc gaacatcttg
     4501 tcgagccagg cgttgatccg atccatgatc ccgtcgggaa gcacgaggtc gagcagaccg
     4561 gagatcccgc cgttgatgat gtcgacgacc gtgtcgatga agccggtgaa gcggtcgaag
     4621 tccgacttcg acttctccgc gttctgcgcg gtcgtgtcat tgccatcggc gtgcctgcgg
     4681 ctcgaggagc ggccaccgct tccagtgttg cgagtcatgt cagaccttct tcctgcttgt
     4741 gctgttcacg ttcagttgcc gagacggctc gaccaggccg gagccgccgg gtcagccggc
     4801 agcgggcggg actcagcatc gatcgaggcc gagaggtgcg actccgccgc gggcgttccg
     4861 atggtcccgt cgacattgaa tcctgggcgg acatccggtc gcgagtcgcc gaccagatcg
     4921 tcgatcccgg ggcggacgtc ggggagaggc tcgcccacga tgcggccgtc agggccgggc
     4981 accggctcgg ggccgccatc gacgttgccg acgggggtgg tggaaccgtc cgtgccggtc
     5041 gaaccatccg taccggtcga accatccgta ccggtcgaac catccgtacc ggtcgaacca
     5101 tccgtaccgg tcgaaccatc cgtaccggtc gaaccatccg taccggtcga accatccgta
     5161 ccggtcgaac
//
LOCUS       DATMSX010000573        10634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_179798,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000573 DATMSX010000000
VERSION     DATMSX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179798"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN041_18505"
     CDS             complement(<1..249)
                     /locus_tag="VN041_18505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWU31078.1"
                     /translation="MTTYLLAGGGTAGHVNPLLAVGDLLRERDGDDVLVLGTREGLES
                     RLVPARGYRLLIVDKVPFPRRPNRAALAFPARFRRAVAQ"
     gene            complement(246..1574)
                     /locus_tag="VN041_18510"
     CDS             complement(246..1574)
                     /locus_tag="VN041_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HWU31079.1"
                     /translation="MTQTAHPPRTESADSAERAGAASRGLSARVSLGRLLTPPSTEFV
                     MIASTAVLLTLFGVVMVLSATNANPKDPYGEALRQGMFAAIGIPLMFIVSRLPVSFFK
                     KIAWPALIFGVLFQLLVFVPGLGVSSYGNKNWIKIAGVQLQPSEFLKLALAVWIAFVL
                     MRKQSKLGTWHQVFIPVVPVAVLAIGTVLAGDDLGTVMVLVLVMLGCLFFSGAKLRLF
                     ILPMILGVIGVLGFALSSKNRMARILAAFDPSTCDAEKECYQAVHGMWGMANGGIFGV
                     GLGNSQEKYWLPAASSDYIFSIVGEELGLLGCLLVLVLFGVFAVGAFHIIRRTSDPFV
                     RIAAGGIVVWIIGQTIFNVGVVLRVFPVMGVPLPFMSKGGTSLLSAMMACGILLAFAR
                     TIPAKQKDAVAAPRNATGSRGSAAAGKTTARRESIAPRKGTASRKGKVAR"
     gene            complement(1543..3078)
                     /gene="murD"
                     /locus_tag="VN041_18515"
     CDS             complement(1543..3078)
                     /gene="murD"
                     /locus_tag="VN041_18515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWU31080.1"
                     /translation="MASERLDGLTSWHSDWTGLRVAVLGLSVTGFSAADTLTELGAEV
                     LVLSEGASEEYARLVPVIGARLELGPLSEVPAVLKDFAPDVIVASPGFPPSHPVIRWA
                     HENDIALWGDVELAWRVRDKVLRADGTPAEWVLITGTNGKTTTTQLTATLLAAGGLRA
                     APCGNIGVPVLDAVRDPSGFEVLVVELSSHQLWYIGQSAPAGQLVPYSATCLNLADDH
                     LVWHGSAEAYRAAKAFVYRHTKVACVYNKSDLATLRMVEEADVTEGARAIGFDLGTPG
                     PSDLGVVDGILVDRAFHDDRRHSALELTTVDALRAAGLAAPHVVQNILAASALARSLG
                     VQPADIRDALQTFRMDAHRIEVVAEHHGIRWIDDSKATNPHAAASSLRAYPGAIWIVG
                     GDLKGVDLGDLVAHAGGTAGGAVVIGVDREPVLAAFRRHAPHVPVQEVVPAETGGVMD
                     RVVEIASGMIDGEGTVLLAPAAASFDQFSSYADRGERFATAVREWIGRGSADDADGSP
                     TAH"
     gene            complement(3083..4192)
                     /gene="mraY"
                     /locus_tag="VN041_18520"
     CDS             complement(3083..4192)
                     /gene="mraY"
                     /locus_tag="VN041_18520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWU31081.1"
                     /translation="MRSLLMATAISLAFSLFLTPVFLKLFRRIGWGQVIRTPEDIANP
                     SHQAKRGTPTMGGVIFILGTIVGYFAGTFFGGMTPGLSAILVLWLMVGFGVVGFIDDY
                     MKVRSQRSLGLSGWRKIIGQLAVLIPFGVVALNFPNSFGQTPASGSVSLFRDIAWLNL
                     FAFGAVLGWILYLIWISVIGVATSNSVNLTDGLDGLAAGSGIFVVGSYGLIAFWQFKQ
                     SCVGEGANTVGCYEVREPLNLAIVAAAFAGGLIGFLWWNAPKAKVFMGDVGSMAIGGV
                     VAAMAILTRTELLLFVIAGIFVLSSGSVILQRAYFKLTRGKRLFLMSPFHHHLEMRGW
                     AEVTIVVRLWIIAGLLAATAVGLFYVNWFAQASNG"
     gene            complement(4189..5601)
                     /gene="murF"
                     /locus_tag="VN041_18525"
     CDS             complement(4189..5601)
                     /gene="murF"
                     /locus_tag="VN041_18525"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWU31082.1"
                     /translation="MIALSLAEIAEILGGELRLTGADTAATVVDGSVDTDSREMTAGS
                     IFVAKPGEITDGHRFVGAAVEAGAVLAIVEHPVDVEVSQIVVADAVQALADLAKAVVA
                     RVRDAGSLRIVGITGSNGKTTTKNLLARILQDEGETVAPIRSYNNEVGAPLTMLRVTD
                     TTRFLVSEFGAAGEGSIARLAGLVEPDVAVVLMVGLAHAGGFGGIEATARAKSELVLA
                     ARAGGTVVLNIDDPRVWAMHDIADGRGMTTVAFGQAPDADVRAEDIAVSADGTSCTVI
                     RGDERLPLHLQVLGAHHVSNALAAIAAAGVLGVPVATAIARLETVEVAERWRMQPMGN
                     ERVRIINDAYNASPDSMAAALRTLAQITGPDERTVAVLGAMSELGESAGEEHDRIGLL
                     AVRLNIQRIVVVGPDARRLYLAAVGEGSWDSEAVHLPDQDAAFEYLKNELRDGDRVLV
                     KSSNSVGLRHLGDRLGELFS"
     gene            complement(5632..7440)
                     /locus_tag="VN041_18530"
     CDS             complement(5632..7440)
                     /locus_tag="VN041_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWU31083.1"
                     /translation="MTTRATRGTRRRTVVALAVILAVLSAFVFRLIDIQVVHADDNVA
                     ASLSKLGGSSPVPGARGDIVDEDGTVLASDAFVYDAQLDPKLIADYEKQTDPKKKPKM
                     PWAEASEKIAAIVGMTGDDVRKLVSDALAKDPNSQYAKLKNGLSTEKYLALRDLGLAY
                     IANKARAIRVYPNGAVAGNIVGFLNGEGKAQYGIERMDQQCLAATNGSISYKRGQGGV
                     RIPGSERIVGAVDGGTVKLTIDSDLNWYLQQMIAEQAQRLGAKAATATVVEVKTGKIR
                     AAAEYPSVDPNNVNTVAPEYWRSHIFSDAYEPGSTFKAITAATVIDQGKATPLSTMTV
                     RSREKFPNGTVINDAFPHPVYNYTLAGALIDSSNVGLSKFGTTVDQTTRADYLKRFGV
                     SQKTAIGFPAEQSGTLPRGRDGKVLDPAKWDGATKYTTTFGQAFTVTPAQIAGAYQAI
                     ANDGKKIDLSLVESCTDADGTVHKATAPQSEQIIKPETAAAVRQMLENVAVQGGNAKT
                     TKVDGYRIGLKTGTAEKSNGQGGYKKGVFFTSMVGIAPIEDPQYVVVVTLDEPTKMRS
                     SAANAAAFQQAMTQVLKTYHVPPSSEPMGPLLPKFN"
     gene            complement(7462..8088)
                     /locus_tag="VN041_18535"
     CDS             complement(7462..8088)
                     /locus_tag="VN041_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31084.1"
                     /translation="MSLLNVRVQPRTETRAAPSRRLRAVIAPAGLRRPKLAYAALAVG
                     GALAIGAAQMIVSLASTQDAFALAKLNNQQHELSLQKQALGDELVALNSPQALAARAD
                     AMGLVVAGAASYLRLSDGAVLGAGDAASWVSTVTPNGPTKVGNALLAPPPAASTETSA
                     DGAGAEGSTADGVPADGAPAADATAQDAATQSDLPPAITDGLPSPTTR"
     gene            complement(8085..9023)
                     /gene="rsmH"
                     /locus_tag="VN041_18540"
     CDS             complement(8085..9023)
                     /gene="rsmH"
                     /locus_tag="VN041_18540"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWU31085.1"
                     /translation="MSLRDIHTPVLLERCIELLAPALAHDGAVLVDATLGMGGHSEAF
                     LERFPHIRLIGLDRDTDALRIAGERLAPFGDRVTLVHSVYDEIGLHAQGASAILFDLG
                     VSSLQLDEADRGFAYSKDAPLDMRMDQTKGRTAATILAEYSEGQLRRIFERYGEEKLA
                     GRYARFIVAARAEKPLTRSGELVDLLVAATPAAAQRAGHPAKRVFQALRIEVNSELSV
                     LADAIPSAMDALNVGGRIAIMAYQSLEDRLVKQAFAAASASTAPAGLPVELPEHAPRF
                     RILTKGAELAGEEERARNPRAIPVRLRAAEKLRESA"
     gene            complement(9122..9553)
                     /gene="mraZ"
                     /locus_tag="VN041_18545"
     CDS             complement(9122..9553)
                     /gene="mraZ"
                     /locus_tag="VN041_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWU31086.1"
                     /translation="MLLGTHTPKLDDKGRVILPAKFREDLAGGVVVTRGQEHCLYVFS
                     TAEFEAQHEKIRQAPLTDKQARSFLRMFLSGASQEMPDSQNRITIPGHLRQYAGLEKE
                     LVVTGVGAHAEIWDASAWNAYLESNEESYSDLEQEVIPGLF"
     gene            complement(9827..10216)
                     /locus_tag="VN041_18550"
     CDS             complement(9827..10216)
                     /locus_tag="VN041_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HWU31087.1"
                     /translation="MPLSEQEQRMLDEMERHLLQHDADVVTAPSGDRALSYRNLIYGA
                     LLLLVGVGGLIAAVVASGRIGAVGSIIIGVVAFLAMLGGAILAFTPVRRPLDGSRPDG
                     APRAQRSRDSSFMDRMNDRWDRRQDEH"
     gene            10310..>10634
                     /locus_tag="VN041_18555"
     CDS             10310..>10634
                     /locus_tag="VN041_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWU31088.1"
                     /translation="MVPSTLVSDAVAARLESFLIRMQADTADYGPDAALFLDAARTTL
                     LGGKRLRARFCHGGWQAAAPAPHTDADPLWGLCAALEIFQSAALVHDDLIDNSDTRRG
                     RPASHR"
BASE COUNT         1685 a         3599 c         3685 g         1665 t
ORIGIN      
        1 ctgcgcgacg gcacggcgga agcgggccgg gaaggccagc gcggcccggt tcgggcgacg
       61 cgggaagggt accttgtcga cgatcagcag ccggtacccg cgggcgggaa ccagacgcga
      121 ctcgagcccc tcccgggtgc ccagcacgag cacgtcgtcg ccgtcgcgct cgcggagcag
      181 atcaccgacg gcgagcagcg gattgacatg gccggcggtg cctccgccgg ccagaagata
      241 ggtggtcacc tggcgacctt accctttcgg gatgcggtgc cctttcgggg tgcgatgctc
      301 tcgcggcgtg ccgtggtctt gccggctgcg gcgctgccgc gcgacccggt cgcgttccgg
      361 ggcgctgcga cggcgtcctt ctgcttcgcc ggtatcgtgc gcgcgaaggc cagcaggatg
      421 ccgcacgcca tcatggccga gagcagcgac gtgccgccct tcgacatgaa cggcagcggc
      481 acgcccatca ccgggaagac gcgcaggacg acgccgacgt tgaagatcgt ctggccgatg
      541 atccagacga cgatgccgcc cgcggcgatc cgcacgaacg ggtcggaggt gcggcggatg
      601 atgtggaagg cgccgaccgc gaagacgccg aacagcacga gcaccagcag gcagcccagc
      661 agtcccagct cctcgccgac gatcgagaag atgtagtcgc tcgatgcggc gggcagccag
      721 tacttctcct gggagttgcc gagcccgacg ccgaagatcc ccccgttggc catgccccac
      781 atgccgtgca cggcctggta gcactccttc tcggcgtcgc acgtcgacgg gtcgaacgcc
      841 gccaggatgc gcgccatgcg gttcttgctg ctgagcgcga aacccagcac gccgatcacg
      901 ccgaggatca tcgggaggat gaacagccgc agcttggcac ctgagaagaa caggcagccg
      961 agcatgacga ggacgagcac catcacggtg ccgaggtcgt ctccggcgag caccgtcccg
     1021 atcgccagca ccgcgacggg cacgacggga atgaacacct gatgccaggt gccgagcttg
     1081 gactgcttgc gcatcagcac gaacgcgatc cacacggcca gtgcgagctt gaggaactcg
     1141 gagggctgga gctggacgcc cgcgatcttg atccagttct tgttgccgta ggacgacacg
     1201 cccaggccgg ggacgaagac gagcagctgg aacagcacgc cgaagatcag tgcgggccat
     1261 gcgatcttct tgaagaagga gaccggcagc ctgctgacga tgaacatcag cgggatcccg
     1321 atggcggcga acatcccctg ccgcagcgcc tccccgtagg ggtccttcgg attcgcattc
     1381 gtggccgaca gcaccatcac cacaccgaag agggtgagca gcacggcggt cgacgcgatc
     1441 atcacgaact cggtcgaggg cggcgtgagc agccttccga gcgagacgcg ggcggacagg
     1501 cccctgctgg cggcgccggc gcgctcggcg ctgtcagcgc tctcagtgcg cggtgggtga
     1561 gccgtctgcg tcatcagcgc tcccccgtcc gatccattcg cgcaccgccg tggcgaagcg
     1621 ctctccccga tccgcgtaac tggagaactg gtcgaaggat gccgccgcgg gagccagcag
     1681 aaccgtgccc tcgccgtcga tcatccccga cgcgatctcc accacgcggt ccatgacccc
     1741 tccagtctca gcggggacga cctcctggac cggcacgtgc ggcgcgtgtc gccggaatgc
     1801 cgccaggacg ggctcgcggt ccaccccgat tacgaccgcg ccgcctgcgg tgccgccggc
     1861 gtgcgcgacg agatcgccga ggtcgacgcc cttgaggtca ccgcccacga tccagatcgc
     1921 gccgggatag gcccgcagcg aggaggccgc ggcatgcggg ttggtcgcct tggagtcgtc
     1981 gatccagcgg atgccgtggt gctccgccac gacctcgatg cggtgggcgt ccatcctgaa
     2041 ggtctgcagc gcatcgcgga tgtccgccgg ctgcacaccc agcgacctgg cgagggcgga
     2101 tgccgcgagg atgttctgca cgacgtgcgg tgcggccagg cccgcggcgc gcagcgcgtc
     2161 caccgtcgtg agctccagcg cgctgtgcct gcggtcgtcg tggaaggccc ggtcgacgag
     2221 gatcccgtcg acgacgccca ggtcgctcgg ccccggagtc ccgaggtcga agccgatggc
     2281 gcgggcgccc tcggtgacat ccgcctcctc gaccatccgc agggtggcga gatcggactt
     2341 gttgtagacg caggcgacct tcgtgtgccg gtagacgaac gccttggccg cacggtacgc
     2401 ctcggcgctg ccgtgccaga ccaggtggtc gtcggcgagg ttcaggcagg tcgcggagta
     2461 cggcacgagc tggccggcgg gagccgactg cccgatgtac cagagctgat ggctggagag
     2521 ctcgaccacc aggacctcga agccgctcgg atcccgaacg gcatccagca ccggcacccc
     2581 gatgttgccg cacggcgccg cgcgcagccc gcccgcggcg agcagggtcg cggtcagctg
     2641 cgtggtggtg gtcttgccgt tggtgccggt gatcagcacc cactcggccg gggtgccgtc
     2701 ggcgcgcagc accttgtcgc ggacgcgcca ggcgagttcg acgtcgcccc acagggcgat
     2761 gtcgttctcg tgggcccacc ggatcaccgg atgcgagggc gggaacccgg gtgaggcgac
     2821 gatgacgtcg ggcgcgaagt ccttcagcac ggcgggcacc tcggagagcg gacccagctc
     2881 gagccgggcg ccgatcacgg gcaccaggcg cgcgtactcc tcggacgcgc cctcggagag
     2941 gacgagcacc tcggcgccga gttcggtgag cgtgtcggca gcggagaagc cggtgacgga
     3001 caggccgagc acggcgacgc gcaggccggt ccagtcggag tgccagctgg tcaggccgtc
     3061 gagacgttcg gatgccatcg cgtcagccgt tcgaggcctg ggcgaaccag ttgacgtaga
     3121 acagcccgac cgcggtcgcg gccagcaggc cggcgatgat ccacagccgc acgacgatcg
     3181 tgacctctgc ccagccgcgc atctccagat ggtgatggaa cgggctcatc aggaacagtc
     3241 gcttgccgcg ggtgagcttg aagtacgccc gctgcaggat gaccgaaccg gaggacagca
     3301 cgaagatgcc ggcgatcacg aacagcagca gttcggtgcg ggtgaggatc gccatcgcgg
     3361 cgaccacgcc gccgatcgcc atcgagccga cgtcacccat gaagaccttg gccttgggcg
     3421 cgttccacca caggaatccg atcaggccgc cggcgaacgc cgccgccacg atcgccaggt
     3481 tcagaggttc gcgcacctcg tagcatccga cggtgttcgc cccctcgccc acgcaggact
     3541 gcttgaactg ccagaacgcg atgaggccgt aggagccgac gacgaagatg ccggagccgg
     3601 cggcgaggcc gtccaggccg tcggtgaggt tgacgctgtt ggacgtggcg acaccgatca
     3661 cggagatcca gatgaggtag aggatccagc cgagcaccgc gccgaaggcg aacaggttga
     3721 gccaggcgat gtcccggaac agcgacacgg agccgctggc aggggtctgg ccgaaggagt
     3781 tcgggaagtt cagcgccacc acgccgaacg ggatgaggac cgccagctgg ccgatgatct
     3841 tgcgccagcc ggacagcccc aggctgcgct gacttcggac cttcatgtag tcgtcgatga
     3901 agccgaccac gccgaagccg accatcagcc acagcacgag gatcgcggac aggccggggg
     3961 tcatgccacc gaagaaggtt ccggcgaagt agccgacgat ggtgccgagg atgaagatca
     4021 cgccgcccat ggtgggggtg ccccgcttgg cctggtggct ggggttggcg atgtcctcag
     4081 gggtgcggat gacctgcccc cagccgatcc tgcggaacag cttcaggaac accggggtca
     4141 ggaagagcga gaaggcgagc gaaatcgccg tcgccataag caaggacctc acgagaacaa
     4201 ttctcccaga cgatcgccga gatgccggag cccgacggag ttcgacgact tcacgagcac
     4261 gcgatcgccg tcgcgcagct cgttcttcaa atattcgaag gcggcatcct gatcgggcag
     4321 gtgcaccgcc tcgctgtccc aggacccctc gcccacggcg gcgagataga gacggcgcgc
     4381 gtcggggccg acgacgacga tgcgctggat gttcagcctc acggccagca gcccgatccg
     4441 gtcgtgctcc tcccctgcgg actcgccgag ctcgctcatg gcgccgagga cggcgaccgt
     4501 gcgctcgtcg gggcccgtga tctgcgcaag cgtgcgcagg gcggcagcca tggagtcggg
     4561 gctggcgttg tacgcgtcgt tgatgatgcg cacgcgctcg ttgcccatcg gctgcatgcg
     4621 ccagcgctcc gccacctcga cggtctcgag acgggcgatc gccgtggcca cgggaacgcc
     4681 gagcacgccc gccgccgcga tggcggcgag ggcgttggag acgtgatgcg caccgagcac
     4741 ctgaagatgc agcggcaggc gctcgtcgcc gcggatgacg gtgcaggagg tcccgtcggc
     4801 cgagaccgcg atgtcctctg cgcgcacgtc ggcgtccgga gcctggccga aggcgacggt
     4861 cgtcatgccg cgcccgtcgg cgatgtcgtg catagcccag acccgcgggt cgtcgatgtt
     4921 cagcaccact gttccgcccg cgcgggcagc gaggacgagc tcggacttcg cccgcgcggt
     4981 ggcctcgata ccgccgaatc ccccagcgtg ggcgagaccc accatgagca ccacggcgac
     5041 gtccggctcg acgagccctg ccaagcgtgc gatgctgccc tcacccgccg cgccgaactc
     5101 gctgacgagg aagcgggtgg tgtcggtgac ccgcagcatg gtcagcggtg cgccgacctc
     5161 gttgttgtac gagcggatgg gggcgacggt ctcaccctcg tcctgcagga tgcgggcgag
     5221 caggttcttg gtcgtcgtct tgccgttgga tccggtgatg ccgacgatcc ggagggagcc
     5281 tgcgtcgcgc acgcgcgcga cgactgcctt ggcgagatcg gccagcgcct gcacggcatc
     5341 cgcgacgacg atctgcgaca cctcgacgtc gaccgggtgc tccacgatgg cgagcacggc
     5401 gcccgcctcg accgcggcgc cgacgaagcg gtgtccgtcg gtgatctctc cgggcttggc
     5461 gacgaagatc gaccctgccg tcatctcccg ggagtccgtg tcgacgctgc catcgacgac
     5521 ggtcgcagcg gtgtccgcac cggtgagtcg cagctcaccg cccaggatct ccgcgatctc
     5581 agcgagtgag agagcgatca tgtggcatct ccatgcacgg cagaacgccg ttcagttgaa
     5641 tttgggaagc agcgggccca tcggctccga cgagggcggg acgtgatagg tcttgagcac
     5701 ctgggtcatc gcctgctgga acgccgcggc gttcgcggcg gaactcctca tcttagtcgg
     5761 ctcgtccaga gtgaccacga cgacgtactg cgggtcctcg atcggcgcga tgcccaccat
     5821 gctggtgaag aagacgccct tcttgtatcc gccctgaccg ttcgacttct cggcggtacc
     5881 ggtcttgagc ccgatgcgat atccgtcgac cttggtcgtc ttggcgttgc cgccctgcac
     5941 ggccacgttc tccagcatct gccggaccgc ggccgcggtc tcgggcttga tgatctgctc
     6001 gctctgcggc gcggtggcct tgtgtaccgt gccgtcggcg tcggtgcagg actccaccag
     6061 cgacaggtcg atcttcttcc cgtcgttcgc aatcgcctga taggcgccgg cgatctgcgc
     6121 cggagtcaca gtgaacgcct gcccgaacgt cgtggtgtac ttggtcgcac cgtcccactt
     6181 cgccgggtcg agaaccttcc cgtcccggcc ccggggcagg gttcccgact gctcggcagg
     6241 gaagccgatc gcggtcttct gactcacgcc gaagcgcttc aggtagtcgg cacgcgtggt
     6301 ctggtcgact gtcgtgccga acttggagag accgacgttc gaggagtcga tcagcgcacc
     6361 cgcgagcgtg tagttgtaca ccgggtgcgg gaaggcgtcg ttgatgaccg tgccgttggg
     6421 gaacttctcc cgcgagcgaa cggtcatcgt cgacagcggc gtcgccttgc cctgatcgat
     6481 cacggtcgcg gccgtgatgg ccttgaacgt cgacccgggc tcatacgcgt ctgaaaagat
     6541 gtgcgagcgc cagtactccg gggccaccgt gttgacgttg ttggggtcga cggagggata
     6601 ttccgccgcg gcccggatct tgccggtctt cacctcgacc accgtcgccg tcgcggcctt
     6661 ggcgcccagt cgctgcgcct gctcagcgat catctgctgc aggtaccaat tcagatcgct
     6721 gtcaatcgtc agcttcaccg ttccgccgtc cacggctccg acgatccgct cgctgccggg
     6781 gattcgcacg ccgccctggc ccctcttgta actgatcgaa ccgttcgtgg cggccaggca
     6841 ctgctgatcc atccgctcga tgccgtactg cgccttgccc tcgccgttga ggaagccgac
     6901 gatgttcccg gcgaccgcgc cgttcgggta cacccggatg gcgcgcgcct tgttggcgat
     6961 gtaggccaga cccaggtctc gaagtgcgag gtacttctcg gtcgacagtc cgttcttgag
     7021 cttggcgtac tggctgttcg ggtccttcgc gagggcgtcc gagacgagct tgcgcacgtc
     7081 gtcgccggtc atcccgacga tcgcggcgat cttctcgctg gcctccgccc acggcatctt
     7141 cggcttcttc ttgggatcgg tctgcttctc gtagtcggcg atgagcttgg gatccagctg
     7201 agcgtcgtac acgaacgcgt ccgaggccag caccgtgccg tcctcgtcga cgatgtcgcc
     7261 gcgggcgccg ggcacggggc tcgagccgcc cagcttgctc agcgaggcgg cgacgttgtc
     7321 gtcggcgtgc acgacctgga tgtcgatgag ccggaagacg aaggccgaga gcacggccag
     7381 gatgacggcg agtgcgacga ccgtgcgccg tcgcgttccg cgtgttgctc gagtcgtcat
     7441 gcggcggtct ccatcctggg actagcgggt ggtcggcgag ggcaggccgt cggtgatggc
     7501 gggaggcaga tcggactggg tcgcggcgtc ctgcgcggtc gcatcggccg ccggggcacc
     7561 gtccgccggc acaccgtcag ccgtgctgcc ttcagcgccg gcgccgtccg ccgacgtctc
     7621 ggtggacgct gcggggggcg gcgcgagcag ggcattgccg accttcgtgg ggccgttcgg
     7681 cgtgaccgtc gagacccagg acgcggcatc cccagcgccg agcacggcgc cgtcgctgag
     7741 ccggaggtac gacgcggctc ccgcgacgac gagacccatg gcgtccgccc ttgccgcgag
     7801 cgcctgcggc gagttcagcg ccaccagctc gtcgcccagc gcctgcttct gcaggctcag
     7861 ttcgtgctgc tggttgttca gcttcgccag cgcgaaggca tcctgcgtcg aggcgagcga
     7921 cacgatcatc tgcgcggcgc cgatcgccag tgccccgccg acggcgagag ccgcataggc
     7981 gagcttcggc cggcgcagcc cggcgggcgc gatgaccgcc cgcagccgac gtgagggtgc
     8041 ggcgcgcgtt tctgtgcgcg gctggacgcg gacgttcaac aggctcatgc cgactccctg
     8101 agcttctcgg cggcgcgcaa tcgcaccggg atggcacgcg ggttgcgagc gcgctcctcc
     8161 tcgcccgcga gctcggcgcc cttggtcagg atgcggaagc ggggggcgtg ctcgggcagc
     8221 tcgacgggga gccctgcggg tgcggtcgag gcggatgccg cggcgaacgc ctgcttgacg
     8281 agccggtcct cgagggactg gtaggccatg atcgcgatgc gaccgccgac gttcagggca
     8341 tccatcgccg acgggatcgc gtcagccagc acactcagct cggagttgac ctcgatccgc
     8401 agcgcctgga agacgcgctt cgcggggtgt ccggcgcgct gcgccgccgc gggcgtcgca
     8461 gcgaccagca ggtcgaccag ctccccggag cgggtgagtg gcttctcggc gcgggccgcg
     8521 acgatgaacc gggcgtaccg tcccgcgagc ttctcctcgc cgtaacgctc gaagatgcgg
     8581 cgcagctgcc cctcgctgta ctcggccagg atggtcgcgg ccgtgcggcc cttggtctga
     8641 tccatccgca tgtccagcgg cgcgtccttc gagtatgcga aaccgcggtc ggcctcatcg
     8701 agctgcagcg aggacacgcc cagatcgaac aggatggccg acgcgccctg cgcgtgcagg
     8761 ccgatctcgt cgtacacgct gtgcaccaga gtgacgcggt cgccgaacgg cgccagccgc
     8821 tcccccgcga tgcgcagtgc atcggtgtcg cggtccaggc cgatcaggcg gatgtgcggg
     8881 aagcgctcca ggaaggcctc ggagtgccct cccatgccga gagtggcgtc gaccagcacg
     8941 gcgccgtcgt gcgccagcgc cggggcgagc agttcgatgc agcgctcgag gagcactggg
     9001 gtgtggatgt cgcggaggct catcgtcttc tatcccgtga cctccggctc tgattcctct
     9061 ccgctcgacc cggcaccggg gaagtgtgtc ggggcagagc ggcgaggcat cacagccgtg
     9121 gtcagaacaa tccgggaatc acctcctgtt ccagatctga gtaggactct tcgttgcttt
     9181 ccaggtacgc gttccaggcg gacgcgtccc agatctcggc gtgggcgccg actccggtga
     9241 cgaccagttc cttctccagt ccggcgtact gacgcagatg gccggggatg gtgatgcggt
     9301 tctggctgtc gggcatctcc tggctggccc ccgagaggaa catgcgcagg aagctgcggg
     9361 cctgcttgtc ggtgagcggt gcctggcgga tcttctcgtg ctgcgcctcg aactcggcgg
     9421 tgctgaagac gtacaggcag tgctcctgac ctcgggtcac gacgacgccg ccggcgaggt
     9481 cctcacggaa cttcgcagga aggatcacac ggcccttgtc atcgagcttc ggagtgtgcg
     9541 tccccagcaa catcggccaa atcaccccct ctgcgtccgg ccaactcgag gtgcgcacca
     9601 ctttactcca cttccctcca ctttcctacg acgatctggg ctcattccca gacttccgcc
     9661 gactcgggac acagaaaccc ccgtgatctc gcgggatcac gggggtggag gacggtggag
     9721 ggccggtgga gggaaaaggg aggatgcggc gcgtcaggac atgaaaaagc ccggatgctc
     9781 tgcatccggg cttcgtctga gtgtcagatc agcactgggg atcggatcag tgctcgtcct
     9841 gtcgtctgtc ccatcgatcg ttcatccgat ccatgaacga ggagtcgcgt gatcgctgcg
     9901 cgcggggggc gccatcgggg cgggacccgt cgagcggacg gcggacgggc gtgaaggcga
     9961 ggatcgctcc acccagcatc gcgaggaacg cgaccacgcc gatgatgatg ctgccgacgg
    10021 ctccgatgcg cccgctcgcg acgacggcgg cgatcagacc gcccacgccg accagcagca
    10081 ggagcgcacc gtagatcagg ttgcggtagc tgagtgcacg gtcacctgac ggcgccgtca
    10141 cgacatcggc atcgtgctga aggagatggc gttccatctc gtcgagcatg cgctgctctt
    10201 gttcggaaag aggcatctcc gacccccttg gttcgttcta tccactctac tcgcctcgga
    10261 gagcgagcgg ctagccaggc gccgtcgaat tcctgcatct aggctgggaa tcgtgccctc
    10321 caccctcgtc tcggacgccg tcgccgcacg ccttgagagc tttctcatcc gaatgcaggc
    10381 cgacacggcc gactacggcc cggatgccgc cctcttcctg gacgcggcgc gcaccactct
    10441 gctcgggggg aagcgcctgc gcgcccgctt ctgccacggc ggctggcagg cggccgctcc
    10501 tgcgccgcac accgacgccg acccactgtg gggcctctgt gcggcgctgg agatcttcca
    10561 gtccgcggcg ctcgtgcacg acgacctgat cgacaactcc gacacccgac gcggacgccc
    10621 ggcgtcccac cgcg
//
LOCUS       DATMSX010000574        13366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_113325,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000574 DATMSX010000000
VERSION     DATMSX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113325"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VN041_18560"
     CDS             complement(<1..1285)
                     /locus_tag="VN041_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU31089.1"
                     /translation="MRAAVRDAAPEPLFLGRDSELADLVRTGRAGLTSGRFLLVRGEG
                     GIGKSTLIDAAAAQAPPDRHVLRGAADAMDRRLTYGLLLDALGPVLTDEDRRVATEHD
                     ERIAGERLLTVIDTATREPTVLILEDLHWADSASLRLLARLSRRLPRLPLVIVASLRP
                     QARHEVVPALDHLLDELGERGLLHSFELGPLPESACIAIAEHLAGAPVGESLARYAAT
                     AGGNPLFLTEMVRSLLRDGAVAVGPDRAELRISPMGPSPSLAMVMMRHLSRLSIDTRE
                     LLSTAALLGARFPAAQLRLVADRPMSDLLPLLREALAAGFLTEIDEDALGFRHQLIQE
                     VLLHDLPHSVRAELHRDVALKLDAAGVPAATVAEHLLRASTARTDLAWMLRLAERTTA
                     SAPGIAVELWGRVLTHSDDADPMHLIATGGIARVAR"
     gene            1499..1654
                     /locus_tag="VN041_18565"
     CDS             1499..1654
                     /locus_tag="VN041_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31090.1"
                     /translation="MAERLARRGRVGAQLLSDTLDLTLATVLAPPFPLTVDRKCDEKD
                     TPPYDER"
     gene            1799..2794
                     /locus_tag="VN041_18570"
     CDS             1799..2794
                     /locus_tag="VN041_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31091.1"
                     /translation="MIQIVWFASACLILSMVLAHFGLRRQRRIDSYDAPAHMSTAGLP
                     ASAVIPSDRRARHRGLAVAWWGLVVVSTAAVGWGLVEMVSNSADEFNLRDLVVLVLAV
                     LWIVGVATFFWTRPALTRGALLAERKPDARVMTVLVGVMNGIACGSALSTACGVTGNW
                     WRARLPMHTTLVVDHRGIEFWRGWIRPRQQYSVPWPHIGGVSPAFVDVGDTNAADMGL
                     GVSVGLRVGTEGWLDIEFRPTRSTFWMTYPYRKSSVVEAFIEAIDELRPARDELFRGV
                     SLKEWKEIVDSAEAEGDQESVDEVLADLREWRDSRSGPLPAGGDWLSGRFADQAD"
     gene            2902..3711
                     /locus_tag="VN041_18575"
     CDS             2902..3711
                     /locus_tag="VN041_18575"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWU31092.1"
                     /translation="MSADLASALLALHHGPGFVIPNAWDAGSARILAQAGFPAIATTS
                     AGIAWSYGLTDGGGTGAERMLQRIAEIVDAVDVPVTADLEDGYGSNPAEVAVTIGRAL
                     EAGAVGANLEDAADGRLFDAGLADEKIAAAREAAPRGTFVINARTDAYFAPNSHVEDP
                     FAETVARAQRYVDAGADCVFVPGVDDAESIRRLASEIPAPLNIVAGLAPTLIDAPTLF
                     SLGVKRVSIGGGLARAALAFVERAGRELREAGTLGFLDGALRYAEVQERFR"
     gene            complement(3714..4745)
                     /locus_tag="VN041_18580"
     CDS             complement(3714..4745)
                     /locus_tag="VN041_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU31093.1"
                     /translation="MSRRVTLADVSQAAGVGIATVSRALGDHPDVSESTRERVREIAT
                     RLGYRPSVAARALRTGGFHAISAIVPDAGWGWWEPVMRAAHATATARGYQLLVHPVDA
                     EGGLTATVDGLANIPTEGVIVVSVPDQDAVRAACDRIGLPAVAIDDTSRATRFPTISA
                     ANRDGARLVVEHLIAQGRRSIALAHGFLGGASPEWGDGLFLEERALGYRDALESAGIA
                     VDERLIIDVADPFDETVTTFPQIDALLTDGPPIDAIFCAADLMAAPVLRSLRASGRAV
                     PDDVAVAGFDDERAALLVDPQLTTVRQPYEQMGATAVDMLLQTIAGEQVPEHRTEIAP
                     RLVVRASSV"
     gene            4864..6129
                     /locus_tag="VN041_18585"
     CDS             4864..6129
                     /locus_tag="VN041_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU31094.1"
                     /translation="MSSPQTAAGTAPAPKPVGAPFIGLYALAMFGIWMSIMLPASVTL
                     ALRISQIDPEGKNVSYSIAAGVGTLVALIANPLFGRLSDRTRSRFGRRRPWIVVGLAG
                     TIVGAIIIGLSGSFPMLLLGWIIMQAFVNAAIAATLAIVPDRVPVKQQGLVGALSGTA
                     SSAAVLVGVFFVQWFPTNVLAQFGLPVLVAVLFAGLLVAMFRDDEPAPADIEPFDFRE
                     FFGSFYINPRTSPDFAKFLVALFLVACGMGVISTYTVYLLQDRVSPPEAELPGLITLA
                     YVIPGVVAVLSAPVAGWLNDRTGRRKAVLGAAAVFIAAGILVLAFATTVPMFMIGLVL
                     VAGLGTGIAYGSYIGFAVATMNNPATAARDLGVVNIALSLPYSVMPFIAPLLLGIGGG
                     GSNYPALLIVSAVLTLLAFVPMLMVRSTR"
     gene            6170..7921
                     /locus_tag="VN041_18590"
     CDS             6170..7921
                     /locus_tag="VN041_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWU31095.1"
                     /translation="MNDYRDSALPIERRVDDLLGRMTVAEKAAMMFHPSTAPAGDGLS
                     QADAVAAAERYAGEVGISHFNVLGGEDSVAVAEWHNTLQEIAEGTRLGIPVTLSTDPR
                     HGFRSNLFTGQAMDSLSRWPDYPGVAAIGETDAARVFGDTMRQELLAMGVRVLLGPMA
                     DIFSEPRWSRGSGTFGEDPERVAELTVAFIEALRGGGELGPHSVAAVVKHFPGGGPQL
                     RGDDAHDPRYPEQVYPGGQQELHLKPFERAFAAGASQVMTYYGKPVGTEWPEVGFAFN
                     EPVVRDILRRRLGFDGIVVTDWNLLESEDMAGMEFGPNGWGLEHLTPLERAAIALDVG
                     VDQFGGDRNAALIEELVDSGRISESRIDESIRRLLREKFRLGVFENRRVDTAATAEIC
                     GSPAFTAAGVEAQRKSLVLLADRGDRIADGARVLLDGVDARTATDAGLVPVHDIAEAD
                     AVVVRLDAPFEPGKGSLGDFFRGGSLEFDAATVAKIARYSASAPVYLSVYLERPAVLT
                     SMTDSSTMLIGDFGAADAVVLDAFRGIAPLTGILPFDIPSSMAAVEASREDVPFDTAD
                     PLFRHGFGLRRESHPRR"
     gene            8046..9050
                     /locus_tag="VN041_18595"
     CDS             8046..9050
                     /locus_tag="VN041_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31096.1"
                     /translation="MTLEKVEAEVRPRPWRPWRSPWAWGALLLLIALPWYLSGVGLDL
                     LPYLLTLVGGWTFGFSFVNLTLRMPQQRGLVLHVVVAVLAAVVVATVIFGDIIPPAGL
                     PDRFRALLVLLQMAAIPAIGWTWLALIGRVTSAVQRPRVEKIVPGFGRENDGSIVRFP
                     AVRMPIRDLRIAVVVLVVVLGGGAVALLIAFDAFVLGPKMTVILLGTVIGLPAYYGLM
                     AYYHRRTIQASVWFAPGRMRIVADAERFEVDFADIRMLRWRTDSEEARVEVRTDAVEL
                     SLIAGLARVPKGAAAALPGLPRHVRRSLELAGLSPGTGRAKGMTTWTRPDSSAPPPPA
                     "
     gene            9062..9601
                     /locus_tag="VN041_18600"
     CDS             9062..9601
                     /locus_tag="VN041_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWU31097.1"
                     /translation="MATGSTIHTFDVQLADVDRGVYDDFTLRMARHPSETDAYMLTRL
                     LAYCLEYEEGIVFTEGVAATDEPAVLVRDLTGRVTAWIEVGAPDATRLHFGSKLADRT
                     TVYTYRDPAKVVANWAGKTIHEADAIVLHSFDPGFLDDAAVALARRNTMTVSVTERQL
                     YLELNGTHLSTTVHDHGIR"
     gene            9660..10166
                     /locus_tag="VN041_18605"
     CDS             9660..10166
                     /locus_tag="VN041_18605"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HWU31098.1"
                     /translation="MPTVTAVHGDITTQQVDAIVNAASNAMRGGGGVDGAIHRAGGPA
                     ILRDCIARFPRGLATGDAGWTTGGDLPSRWVVHTVGPNWNAGQRDRELLVSCYRRSLQ
                     VADELGARSIAFPLISAGVYGWPREDAIRVAVDTILATEVQLDDARIVAFDESTFQAV
                     QATIRAVH"
     gene            complement(10172..11542)
                     /locus_tag="VN041_18610"
     CDS             complement(10172..11542)
                     /locus_tag="VN041_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU31099.1"
                     /translation="MPTFTPLQRLVVSVAILASFVTFLDGTVVNVALPAISRELGGGI
                     ATQQWVVDAYLITLSSLILLAGSVSDAYGRVLVMRIGLIAFGVASVAVAAAPDPLILI
                     IARAAQGAAGALLVPSSLALITATMRGEVQSRAIGVWTAFTTGAQLVGPVMGGMFVDL
                     LSWRFVFLINVLPIAVNLILLGRLHLPAHQRTTRVDWWSGVLCAIGLGGLVFALIEQE
                     RMGWSAAAIWIPGALGILLLVAFLLRQRSSDTALMPLSLFTVRNFAWGNLATLFIYAA
                     LSLNGFVIGVYLQQGPGLSATAAGLASLPITILMILLSSRAGAWAGKLGPRLFMTVGP
                     LLMAVGAALLLTVGVEFDYWWQVLPAMLVLGVGLSLTVAPLTSAILGAIDENRSGIAS
                     AVNNAVSRVAGLLVVALLSTIVGGALDLNGFHSAALVTAGLFALGGVVSWIGIRRNPV
                     EEPAAS"
     gene            11693..12094
                     /locus_tag="VN041_18615"
     CDS             11693..12094
                     /locus_tag="VN041_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31100.1"
                     /translation="MDSMMMDMMSKDMQMPGMEMMDMAAMQACMDACSAGEQACTVCS
                     MQMMDCGPACMNCADMCNTMMRGMMRMQGMTPAVMMSMLDACIAMCQYCMDKCMEHAD
                     MSEVCKMCAQACKACMDACEAMRESMMKMAS"
     gene            complement(12155..12499)
                     /locus_tag="VN041_18620"
     CDS             complement(12155..12499)
                     /locus_tag="VN041_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31101.1"
                     /translation="MTSPQTRLSPLEVRERAACMCDHGYACSSFAPGHALHLIQARLA
                     SATPLGWVDALVDSADATTGEIVLRTLDGDVVTVWNAGGAALEASSGAPVALHRDYAV
                     LAVGATKYNVAR"
     gene            complement(12598..12912)
                     /locus_tag="VN041_18625"
     CDS             complement(12598..12912)
                     /locus_tag="VN041_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31102.1"
                     /translation="MTLPNDHPHREPGQRGIGTGLLIGLAFHAFGLLLALGSLSVMSQ
                     IGLFALLWPFIAIVVISVIVMATAGAHHERRRIAGGVLIISAAVWLIIIGPCVGLVYS
                     IP"
     gene            complement(12926..>13366)
                     /locus_tag="VN041_18630"
     CDS             complement(12926..>13366)
                     /locus_tag="VN041_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31103.1"
                     /translation="DPAPPPPPAYDPSQVVPPPPPDPPAPGYPNAPDVPAPGYPPQHP
                     SPFPVDPAQAAPGYYDPNQQPGYYPGANQQPGYYDPAYGVNPLGLVDERGYPIGYTPP
                     QPKGMSGGVKGVLIGCGVVAAVGLIGVFGFVIVILGPCIGLNTY"
BASE COUNT         2018 a         4559 c         4751 g         2038 t
ORIGIN      
        1 cacgcgcgac gcgggcgatc cccccggtgg cgatcaggtg catcgggtcg gcgtcgtcgc
       61 tgtgggtgag cacccgcccc cacagctcca cggcgatgcc cggggccgat gcggtggtgc
      121 gctcggcgag ccgcagcatc cacgccagat ccgtgcgggc cgtggatgca cgcagcaggt
      181 gctcggcgac ggtggccgcg ggcacgccgg cggcatccag cttcagagca acatcgcggt
      241 gcagctcggc gcgcaccgag tgcggcaggt cgtgcagcag cacctcctgg atgagctgat
      301 gccggaaccc gagggcatcc tcgtcgatct cggtgaggaa tcccgctgcg agcgcttccc
      361 gcagcagcgg gagcaggtcg ctcatcggcc gatcagccac cagtcgcagc tgcgcggcgg
      421 ggaaccgcgc gccgagcagc gccgcggtgg acagcagttc gcgcgtgtcg atgctgagcc
      481 ggctgagatg gcgcatcatc accatggcga gtgaggggga ggggcccatc gggctgatgc
      541 gcagctccgc tcgatccgga cccacagcga cggcgccgtc gcgcagcagc gatcgcacca
      601 tctcggtgag gaacagcggg ttgccgcccg ccgtcgccgc ataacgcgcg agggattcac
      661 ccactggggc tcccgcgagg tgctcggcga tcgcgatgca ggcgctctcc ggcagcggac
      721 cgagctcgaa ggagtgcagc aggccgcgct cgcccaactc gtcgagcagg tggtccagcg
      781 cgggaacgac ttcgtggcgg gcctgcggcc gcagcgaggc cacgatcacc agaggcagcc
      841 gcggcaacct gcgcgagagg cgagcgagca ggcgcagcga ggcgctgtcc gcccagtgca
      901 gatcctccag gatcagcacc gtcggctccc gggtggccgt gtcgatcacc gtcagcagcc
      961 gttcgcctgc gatgcgctcg tcgtgctccg tcgcgacgcg tcgatcctcg tctgtgagca
     1021 cgggcccgag cgcgtccagc agcaggccgt acgtgagacg tcgatccatc gcatccgccg
     1081 cgccgcgcag cacgtgccgg tccggcgggg cctgggctgc cgcggcgtcg atgagcgtgc
     1141 tcttgccgat gccgccctcg ccgcgcacca gcaggaaacg gccgctcgtg agaccggcgc
     1201 ggccggtcct gaccaggtcg gcgagctcgg agtcgcggcc gaggaagagg ggctcggggg
     1261 cggcgtcacg cacggctgcg cgcatcggtg gtaccgcctc tctgccccag acagcccccc
     1321 ggatatctgg attctagcga gggaggttac ccgtgtggca tgaatcttgc gacggcgcag
     1381 atgcggctgc ggcgttcgcc ccgaggctgg taggcgaatg gctggccgac cggcccgagc
     1441 agcggcatcg gcggatgccc gggacgatgg tgttctccga catctcggat tcacgcgcat
     1501 ggccgagcgc ctggcccggc gcggacgcgt cggcgcacaa ctgctcagcg acaccctcga
     1561 cctgaccctc gcgacggtgc tcgccccgcc cttccccctc acggtcgaca gaaagtgcga
     1621 cgagaaggac actccgccct acgatgagcg atgatggcgc gatggagcgg catggacagg
     1681 agaattcgtg actcgcccac cgacgagatc ctccgagttc ctgcgcttct acctgcggtc
     1741 ggggtatccg ctcatggtgc tgggagtcat cgtcgtcggt ctcctgaggg gactgaccat
     1801 gatccagatc gtctggttcg cgtccgcctg tctcatcctc agcatggtcc tggcgcactt
     1861 cggtctgcgc aggcagaggc gcatcgactc ctacgacgcc ccagctcata tgtcgacagc
     1921 cgggcttccg gcgtccgccg tcatcccatc ggaccgtcgc gcgaggcacc gtggcctggc
     1981 cgtcgcgtgg tggggtctcg tcgtcgtgag caccgcagcg gtcggctggg ggctggtcga
     2041 gatggtctcg aactcggccg acgaattcaa cctgcgcgac ctcgtagtgc tcgtcttggc
     2101 cgtgttgtgg atcgtgggtg tagcgacgtt cttctggact cgcccggcgc ttactcgagg
     2161 tgcccttctc gccgagagga agccggatgc ccgggtgatg actgtccttg tcggcgtaat
     2221 gaacggaatt gcctgcggtt ctgctctcag caccgcgtgc ggcgtcaccg ggaactggtg
     2281 gcgtgcgcga ctgccgatgc acaccacgct ggtcgtcgat catcgaggga tcgaattctg
     2341 gcgcggttgg atccgtcctc gtcagcagta ctccgtcccg tggccacaca ttggcggggt
     2401 ttctccggcg ttcgtcgacg tcggagacac caatgccgcc gatatgggcc tgggggtcag
     2461 cgtgggtctc cgggtcggaa ccgaggggtg gctcgacatc gaattccgcc ccacacgcag
     2521 cacgttttgg atgacctatc cgtaccggaa gtcgtcggtg gtggaagcct tcatcgaggc
     2581 gatcgacgag ttgcggcctg ctcgcgatga actgttccgc ggagtgagtc tcaaagagtg
     2641 gaaggagatc gttgattcag cggaggccga aggcgaccag gagagcgtcg acgaggtcct
     2701 ggccgacctg cgagagtggc gcgactcgcg cagcggcccg ttgcctgctg gcggcgactg
     2761 gctgtctggg aggttcgcgg accaggccga ctgagcgtgg ccagcgtccg ctgccggagt
     2821 gcgttcgacc cgcgaggcga gaagcttgac ctgactgcgc acggggcgtg tgatggtcgt
     2881 gcacgattcg aaggggtcac catgtccgca gatctcgcct ccgccctgct cgccctgcat
     2941 cacgggcccg gcttcgtgat cccgaacgcc tgggacgcgg ggtccgcgcg catcctcgcg
     3001 caggctggct ttccggcgat cgcgaccacc agcgccggga tcgcgtggtc gtacgggctg
     3061 accgacggtg gtgggaccgg agccgagcgg atgctgcagc gcatcgccga gatcgtcgac
     3121 gcggtcgacg tgcccgtcac cgccgatctc gaagacggct acggctcgaa cccggcggag
     3181 gtggcggtca cgatcggtcg tgctctcgag gccggggcgg tcggcgccaa cctggaggat
     3241 gccgcggacg gccgcctgtt cgacgcgggt ctggcggacg agaagatcgc cgccgcgcgg
     3301 gaagcggctc ctcggggcac gttcgtgatc aacgcccgca ccgacgccta cttcgcgccg
     3361 aactcgcacg tcgaggatcc gttcgcggag accgtggctc gcgcacagcg ctatgtcgat
     3421 gccggtgccg actgcgtctt cgtcccgggc gtggacgacg ccgagagcat ccgccgactg
     3481 gcatccgaga ttcctgcacc gttgaacatc gtcgcggggc tcgcgccgac gctcatcgac
     3541 gcgccgaccc tgttctcact gggagtcaag cgggtgagca tcggcggcgg gctcgcccgc
     3601 gctgccctcg cgttcgtcga gcgcgccggt cgtgaactgc gtgaggcggg caccctcggg
     3661 ttcctcgacg gagctctccg ctacgccgag gtgcaggaac ggttccgctg agctcagacg
     3721 gaggacgcgc gaacgaccag cctcggcgcg atctcggtgc ggtgctccgg tacctgctcg
     3781 ccggcgatgg tctgcagcag catgtcgacc gcggtcgcgc ccatctgctc gtagggctgc
     3841 cgcaccgtcg tgagctgggg atcgacgagc agcgccgcgc gctcgtcgtc gaagccggcg
     3901 acggcgacat cgtcgggcac cgcccggccg gaggcacgca gggaccgcag caccggcgcg
     3961 gccatcagat cggcggcgca gaagatcgcg tcgatcggcg gtccgtcagt gaggagcgcg
     4021 tcgatctgcg ggaaggtcgt gaccgtctcg tcgaaggggt cggcgacgtc gatgatcagg
     4081 cgctcgtcga cggcgatccc ggccgattcc agggcgtcgc ggtagccgag cgcgcgctcc
     4141 tcgaggaaga ggccgtcgcc ccactccggg ctcgcgccgc cgaggaagcc gtgcgcgagg
     4201 gcgatggagc gccgcccctg ggcgatgagg tgctcgacca ccaggcgggc gccgtcgcgg
     4261 ttggccgccg agatggtcgg gaaccgcgtg gcacggctgg tgtcgtcgat cgcgacggcg
     4321 ggcaggccga tgcgatcgca cgccgcacgc acggcatcct gatccggcac gctcaccacg
     4381 atcacgccct cggtggggat gttggcgagc ccgtccacgg tggcggtgag tccgccctcg
     4441 gcgtcgacgg ggtgcacgag cagctggtac ccgcgggcgg tcgcagtggc gtgcgcggcg
     4501 cgcatcaccg gctcccacca tccccacccc gcgtcgggca cgatcgcgct gatggcgtgg
     4561 aagccgccgg tgcgcagggc gcgcgccgcc acggacgggc gatagccgag gcgggtcgcg
     4621 atctcgcgca cgcgctcccg ggtggactcg ctcacatcgg ggtggtctcc gagcgcgcgt
     4681 gagaccgtgg cgatgccgac tccggcggcc tgggagacat cggccagggt gacccgtcgc
     4741 gacatgtcgg ctccttcggc tcttgacacg gaatcgtcct tcgacatatc ttatggtgac
     4801 gattccggaa acgttaccgg taacgtcatc aatacccgat catcgctcaa cgacgaggac
     4861 cctatgtcat ctccccagac cgccgccggc acggcacccg ctcccaagcc ggtcggcgct
     4921 cccttcatcg ggctgtacgc cctggccatg ttcggcatct ggatgtcgat catgctgccc
     4981 gcctcggtga ctctcgccct gcgcatctcg cagatcgacc ccgagggcaa gaacgtcagc
     5041 tactcgatcg ccgccggcgt cggcacgctc gtcgcgctca tcgccaaccc gctgttcgga
     5101 cgactgagcg atcgcacccg cagccgcttc gggcgtcgcc gcccgtggat cgtggtcggg
     5161 ctcgccggca ccatcgtcgg cgcgatcatc atcggcctga gcggctcgtt cccgatgctg
     5221 ctgctgggct ggatcatcat gcaggcgttc gtgaacgcgg cgatcgccgc gacgctcgcg
     5281 atcgtccccg accgcgtgcc ggtgaagcag cagggcctgg tcggcgcgct ctccggcacc
     5341 gcctcctcgg ccgcggtcct ggtcggcgtc ttcttcgtgc agtggttccc caccaacgtg
     5401 ctcgcccagt tcggactgcc cgtgctcgtc gccgtgctgt tcgccgggct gctggtggcc
     5461 atgttccgcg acgacgagcc cgctccggcg gacatcgagc ccttcgactt cagggagttc
     5521 ttcggcagct tctacatcaa cccccgtacg tcgcccgact tcgcgaagtt ccttgtcgcg
     5581 ctgttcctgg tggcatgcgg aatgggcgtg atctccacct acacggtgta cctgctgcag
     5641 gaccgcgtct ccccgcccga ggccgagctg cccggcctga tcaccctcgc ctacgtcatc
     5701 cccggcgtcg tcgcggtgct cagcgccccg gtcgcgggct ggctcaacga ccgcaccggc
     5761 cgtcgcaagg ccgtgctcgg tgccgccgcc gtgttcatcg ccgccggcat cctggtgctc
     5821 gcgttcgcca ccacggttcc gatgttcatg atcggactcg tcctcgtcgc cggactcggc
     5881 acgggcatcg cctacggcag ctacatcggg ttcgccgtgg ccaccatgaa taaccccgcc
     5941 accgcggccc gcgacctggg cgtcgtgaac atcgccctct cgctgccgta ctccgtcatg
     6001 ccgttcatcg cgccgttgct cctgggcatc ggcggaggcg gatcgaacta ccccgcgctg
     6061 ctgatcgtca gcgccgtgct cacgctgctc gcgttcgtgc cgatgctgat ggtgcgctcc
     6121 acgcgctgac cggcgcacgg caaccgaccc gacgacctga aggactgaca tgaacgacta
     6181 ccgcgattcc gctctgccca tcgagcgccg cgtcgacgac ctcctcggac gtatgaccgt
     6241 ggccgagaag gcggcgatga tgttccaccc ctcgacggcg ccggccggtg acggcctgag
     6301 ccaggcggat gccgtcgccg cggcggagcg gtacgcggga gaggtcggca tcagccactt
     6361 caacgtcctc ggcggcgagg acagcgtcgc cgtggccgag tggcacaaca ccctgcagga
     6421 gatcgcggag ggaacccgtc tcggcatccc ggtcacgctg tcgaccgatc cccggcacgg
     6481 cttccgcagc aacctgttca cgggacaggc catggacagc ctgtcgcgct ggcccgacta
     6541 cccgggtgtc gcggcgatcg gcgagacgga cgccgcgcgc gtcttcggcg acactatgcg
     6601 ccaggagctg ctggcgatgg gcgtgcgggt gctgctcggt ccgatggccg acatcttcag
     6661 cgagccgcgg tggtcgcgcg gttcgggcac cttcggtgag gaccccgaac gcgtcgccga
     6721 gctcacggtc gccttcatcg aggcgctgcg cggcggtggc gagctggggc cgcactcggt
     6781 cgcggcggtc gtgaagcact tccccggagg cggtccccag ctgcgcggag acgacgcgca
     6841 cgacccgcgc taccccgagc aggtgtaccc gggcggacag caggagctgc acctgaagcc
     6901 gttcgagcgc gcgttcgccg cgggcgcgag ccaggtgatg acctactacg gcaagcccgt
     6961 cggaaccgaa tggcccgagg tgggcttcgc gttcaacgag cctgtcgtcc gcgacatcct
     7021 gcgccggcgc ctcgggttcg acggcatcgt cgtgaccgac tggaacctgc tggagtccga
     7081 ggacatggcg ggcatggagt tcggtccgaa cggttggggg ctcgagcatc tcaccccgct
     7141 ggagcgcgct gcgatcgcgc tcgacgtggg ggtggatcag ttcggcggcg accgcaatgc
     7201 ggccctgatc gaggagctcg tcgactccgg gcgcatctcc gagtcccgca tcgacgagtc
     7261 gatccgccgg ctgctgcgcg agaagttccg cctgggcgtg ttcgagaacc gacgggtcga
     7321 caccgcggcg accgcggaga tctgcggttc gccggcgttc acggcggccg gggtggaggc
     7381 gcagcggaag tcgctcgtgc tgctcgccga tcgcggcgac cgcatcgcgg acggggcgcg
     7441 cgtgctgctc gacggcgtcg acgcccggac cgcgaccgat gcgggacttg ttcccgtcca
     7501 cgacatcgcc gaagcggatg ccgtggtcgt gcgcctggat gcccccttcg aaccagggaa
     7561 gggatcgctc ggcgacttct tccgcggcgg cagcctggag ttcgatgcgg ccaccgtcgc
     7621 gaagatcgcc cggtactcgg cctcggcgcc ggtctacctc tcggtgtact tggagcgacc
     7681 ggccgtgctc acgtcgatga ccgacagctc gacgatgctc atcggtgact tcggcgcggc
     7741 cgacgccgtc gtgctggatg ccttccgcgg catcgcgccg ctcaccggca tcctgccctt
     7801 cgacatcccg tcgtcgatgg ccgcggtgga ggcgtcccga gaggacgtgc cgttcgacac
     7861 cgccgatccg ctgttccgtc acggattcgg gctgcgcagg gagagccacc cgcgtcgctg
     7921 acgctcgggg cggtgctgtg ccatcctgat cggatgactc tcgcccgcct gcacgcccgt
     7981 ggcgagacag ccgtgctcgc gtggctggtg ctgtggccgc gcgatcgcgc gacggagctc
     8041 gccgagtgac gctcgagaag gtggaggccg aggtccgtcc ccggccctgg cgtccgtggc
     8101 gcagtccgtg ggcgtggggt gccctgctgc tgctcatcgc gttgccgtgg tacctctccg
     8161 gtgtcggcct cgacctgctg ccgtacctgc tcacgctcgt cggcggctgg accttcgggt
     8221 tctcgttcgt gaacctcacc ctgcggatgc cgcagcagcg cgggctcgtg ctgcacgtgg
     8281 tggtcgccgt gctggccgcc gtcgtcgtgg ccacggtgat cttcggtgac atcatcccgc
     8341 cagcgggcct cccggaccgg ttccgggcgc tgctcgtgct gctgcagatg gcggcgatcc
     8401 cggcgatcgg ctggacctgg ctcgccctga tcggccgcgt cacgagcgcc gtgcagcgac
     8461 cgcgggtgga gaagatcgtg ccgggcttcg ggcgcgagaa cgacggctcg atcgtgcgct
     8521 tccccgccgt gcgcatgccc atccgagacc tgcggatcgc cgtggtcgtc ctcgtcgtcg
     8581 tgctcggtgg aggcgccgtc gccctgctga tcgcgttcga cgcgttcgtg ctggggccca
     8641 agatgacggt catcctgctc ggcacggtga tcgggctccc cgcctactac ggactgatgg
     8701 cctactacca tcggcgcacg atccaggcgt cggtgtggtt cgcgccgggc aggatgcgga
     8761 tcgtcgcgga cgccgagcga ttcgaggtgg acttcgccga catccggatg ctgcgctggc
     8821 gcaccgacag cgaggaggcg cgcgtggagg tgcgcacgga tgccgtggag ctctcgctca
     8881 tcgcgggtct cgcccgcgtg ccgaagggcg ccgcggcggc gctgcccggg ttgccgcgac
     8941 atgtgcggcg ctcgctggaa ctggccgggc tgtcgcccgg gaccgggcgt gcgaagggga
     9001 tgacgacctg gacgcgtccc gattcgagcg ccccgccacc tcccgcgtag gctcgacact
     9061 catggcgaca ggctccacga tccacacgtt cgacgttcag ctggcggatg tggaccgcgg
     9121 tgtctatgac gacttcacgc tgcgcatggc gcggcatccc tccgagaccg acgcctacat
     9181 gctcacccgc ctgctggcgt actgcctgga gtacgaggag ggcatcgtgt tcaccgaggg
     9241 cgtcgccgcg accgacgaac ccgccgtgct cgtgcgcgat ctcaccggac gggtgaccgc
     9301 gtggatcgaa gtgggcgcgc ccgacgccac ccgcctgcac ttcggcagca aactcgccga
     9361 ccgcaccacg gtgtacacct accgcgaccc cgccaaggtc gtcgcgaact gggcgggcaa
     9421 gaccatccac gaggcggatg ccatcgtgct gcacagcttc gatccgggct tcctcgacga
     9481 tgccgcggtg gcgctggcgc gccgcaacac gatgaccgtg tcggtcaccg agcggcagct
     9541 gtacctggaa ctcaacggca cgcacctcag cacgaccgtg cacgaccacg gcatccgctg
     9601 acctcagccc gacgcgggca cgggttccgc cgggcatccg gccggagcag actggtcgca
     9661 tgcccaccgt caccgccgtc cacggcgaca tcacgaccca gcaggtcgat gcgatcgtca
     9721 acgccgcgag caacgcgatg cgcggcggcg ggggagtcga cggcgcgatc caccgtgccg
     9781 ggggaccggc gatcctgcgg gactgcatcg cccgattccc acggggactc gcgaccggcg
     9841 acgccggctg gacgaccggc ggcgacctgc cctcgcgatg ggtggtgcac acggtcggtc
     9901 cgaactggaa cgccgggcaa cgcgatcgcg agctgctggt ctcctgctat cgccgctccc
     9961 tgcaggtcgc cgacgagctg ggagcgcgca gcatcgcgtt cccgctgatc agtgccggcg
    10021 tgtacggttg gccgcgggag gacgcgatcc gggtcgcggt ggacacgatc ctggcgaccg
    10081 aggtgcagct ggacgatgcc cggatcgtgg cgttcgacga gtcgaccttc caggcggtgc
    10141 aggccacgat ccgcgcggtg cactgagccg atcaggaagc ggcgggctcc tcgaccgggt
    10201 tgcggcggat gccgatccac gacaccacgc ctcccagcgc gaacagaccg gccgtgacca
    10261 gcgcggcgct gtggaagccg ttcagatcga gcgctccgcc gacgatcgtg gacagcagcg
    10321 ccaccaccag cagtccggcg acgcgcgaga cggcgttgtt gacggctgaa gcgatgccgc
    10381 tgcggttctc gtcgatcgcc ccgaggatcg ccgaggtgag gggtgccacg gtcagcgaga
    10441 gccccacgcc cagcacgagc atcgccggca ggacctgcca ccagtagtcg aactcgacgc
    10501 cgacggtcag cagcagcgcg gcgccgaccg ccatcagcag ggggccgacg gtcatgaaca
    10561 ggcgcggccc gagcttgccg gcccaggcac ccgcgcggga gctgagcagg atcatcagga
    10621 tcgtgatggg gaggctcgcg agccccgccg ctgtcgcgct cagcccgggg ccctgctgca
    10681 ggtagacgcc gatcacgaag ccgttcagcg agagggcggc gtagatgaac agcgtggcca
    10741 ggttgcccca ggcgaagttg cgcacggtga acagcgagag gggcatgagc gcggtgtccg
    10801 aggaccgctg gcgcagcagg aaggcgacga gcagcaggat gccgagcgct ccggggatcc
    10861 agatcgcggc ggccgaccag cccatccgct cctgctcgat cagcgcgaac acgagcccgc
    10921 cgagaccgat cgcgcagagc acgccgctcc accagtccac cctcgtggtg cgctggtgcg
    10981 cgggcaggtg aagcctgccg agcaggatca ggttcaccgc gatcggcagc acgttgatca
    11041 ggaacacgaa ccgccacgag agcagatcca cgaacatgcc gcccatcacg ggtccgacga
    11101 gttgcgcacc ggtcgtgaac gccgtccaca cgccgatcgc gcgggactgc acctcgccgc
    11161 gcatcgtggc ggtgatgagc gcgagcgagc tcggcaccag cagggcaccc gccgcgccct
    11221 gcgctgcgcg cgcgatgatg aggatcagcg ggtcgggcgc ggcggcgacg gcgacggatg
    11281 ccacgccgaa ggcgatcagg ccgatccgca tcacgagcac gcggccgtag gcatccgaga
    11341 ccgagcccgc cagcagaatc agcgagctga gcgtgatcag gtacgcgtcc accacccact
    11401 gctgcgtggc gatgccgcca ccgagctccc ggctgatggc cggcagcgcc acgttgacca
    11461 ccgtgccgtc gaggaaggtc acgaacgagg cgaggatcgc gaccgagacg accagtcgct
    11521 gcagcggggt gaacgtcggc acggaccgac cctacatccg gtgagggacg tcgggggcgg
    11581 aagagcggaa tcggccatcg acggagtgat ccggcaggcg taatctgacc atcaggcggg
    11641 tccacctgcc cgcccgaagc cggcgagagc cgatgatgag gagcggtgcg cgatggactc
    11701 gatgatgatg gacatgatgt ccaaggacat gcagatgccg ggcatggaga tgatggacat
    11761 ggccgccatg caggcctgca tggatgcgtg ttcggccggt gagcaggcct gtacggtctg
    11821 ttcgatgcag atgatggact gcggtcccgc ctgcatgaac tgcgccgaca tgtgcaacac
    11881 gatgatgcgc ggcatgatgc ggatgcaggg catgaccccg gccgtgatga tgtcgatgct
    11941 cgacgcctgc atcgccatgt gccagtactg catggacaag tgcatggagc acgccgacat
    12001 gagcgaggtc tgcaagatgt gcgctcaggc gtgcaaggcc tgcatggacg cgtgcgaggc
    12061 catgcgcgag tcgatgatga agatggcgtc ctgaccgcgg ttccctgagc cgcgggtccc
    12121 tgaatcctgg gtccctgagc ccgtcgacgg gccctcagcg cgcgacgttg tacttcgtcg
    12181 cgccgaccgc cagcacggcg tagtcgcggt gcagcgccac gggagcacca gaggatgcct
    12241 cgagcgcggc gccgccggcg ttccagacgg tgacgacgtc gccgtcgagc gtgcgcagca
    12301 cgatctcgcc ggtcgtggca tctgcggagt cgaccagagc atccacccag ccgagcggcg
    12361 tcgccgaggc gagacgcgcc tggatcaggt gcagggcgtg ccccggggcg aacgaggagc
    12421 aggcgtaccc gtggtcgcac atgcacgcag cacgctcacg cacctcgagg ggcgaaaggc
    12481 gggtctgcgg gctggtcacg gtgatctcct cacgatcggt tgatccgagg ctacgaccgc
    12541 tctgccggcg cgtcgatcac gcgcgaaacg tgaggaaaca cttctcccag gccgtgctca
    12601 ggggatcgag tacacgagtc cgacgcacgg cccgatgatg atcagccaca cggccgcaga
    12661 gatgatgagc acgccgcccg cgatacgccg gcgctcgtgg tgcgcaccgg cggtcgccat
    12721 gacgatcacg gatatgacga cgatcgcgat gaacggccag agaagggcga agaggccgat
    12781 ctgagacatc acgctcagcg acccgagggc gagcagcagg ccgaacgcgt ggaaggcgag
    12841 cccgatcagc agcccggtgc cgatgccgcg ctggccgggc tcgcggtgag gatgatcgtt
    12901 gggcagggtc acgcgccggc tccgatcagt aggtgttcag accgatgcag gggccgagta
    12961 tgacgatcac gaatccgaac accccgatga ggccgaccgc cgcgacgacg ccgcagccga
    13021 tcagtacgcc cttgactccg ccggacatcc ccttcggctg gggtggcgtg tacccgatcg
    13081 ggtagccgcg ttcatcgacg agcccgagcg gattgacgcc gtacgcggga tcgtagtagc
    13141 cgggctgctg attcgctccg ggatagtagc cgggctgctg attcggatcg taatagccgg
    13201 gagccgcctg ggccggatcg acagggaacg gcgacgggtg ctgcggggga tagcccggcg
    13261 cgggcacgtc gggagcgttc ggatagccgg gcgccggtgg atccggggga ggaggcggca
    13321 ccacctggga gggatcgtag gcaggcggcg gaggcggcgc ggggtc
//
LOCUS       DATMSX010000575         8280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_141729,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000575 DATMSX010000000
VERSION     DATMSX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141729"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2565)
                     /locus_tag="VN041_18635"
     CDS             complement(<1..2565)
                     /locus_tag="VN041_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWU31104.1"
                     /translation="MNRTKGIAAALCAATALTLSPAGPAHADVPVGDPVAYTAYQPIH
                     DPGANAPDYFQPSWFDDNGRHIQAHGGAVVSAQELGVEGGDVVAGSEQGRTVYYWYGE
                     DRSSGYYGSPGVHAYKSYDTLNWTDEGIVLRSVSSAAELEDDYFDALYDTVDESGQPR
                     TDRIAELNYHLNTADAADYTTIFERPKVLHNESTGKWVLWWHSDGQTSPGGSMYARSM
                     AGVAVSDSPTGPFRMTGVYRLPNRTDYKACISAAVPGQARDMTLFQDADGTAYIVYSS
                     EENRSLYIAQLDASYTNVTHTTGTDMANAHQYSEDGRYPYLFADGTADAPVRGEDFQI
                     VKECGMLEAPALFTHGGRYYAVASGATGWAPNPQTYHTADSILGTWIRGVEADDPFEN
                     VSYSSVPEGGDGLLSVGDTRRSTFGSQSTNVLDLGGGRFVYMGDRWNSGAANSTYVWL
                     PITIGENGRAEMRNPATEDPARWGAGWDASYFDAHGRGTEIWSVVDDGLPKTVEPGED
                     FSDALPATVPVKVGSTTVDTAVTWSATSFAERGTQRIVGTLAADAQFTAGRTFTRTIE
                     VATPGIVDLALESAVSASSRQSLAATVADGDVKGKGWDDWVGGGVYPKSSWLSFTWPL
                     TQKVDEVVVHMYKDGSGATWPSRIVAEYLDASGDWVPTGAGVDVAQDPSASAPVAALD
                     VSGIPQTSGIRLHLTTATNTWQSISEVQIWGADGVEDVCSAEGTTISASFHQTEWETM
                     PPQNACDGRASTSWSTWTGGTPKDSATFTVQPKTATVVDRVGFTNIEGTIRDVSVEYR
                     DAQGTWHPTSAQHVAPSGNGRPTQLAFDPVLASAVRLAFSTPGSYLKIPTLAVGARD"
     gene            complement(2701..4221)
                     /locus_tag="VN041_18640"
     CDS             complement(2701..4221)
                     /locus_tag="VN041_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HWU31105.1"
                     /translation="MSSSRITIDREFTIADVPRRLFGSFVEHMGRCVYTGIFEPGHPQ
                     ADERGFRRDVLELVREMGPTVVRYPGGNFVSGYRWEDGVGPVADRPVRIDGAWHTIET
                     NAFGLHEFVDWARDADVEVMEAINLGTRGVEEARALVEYANHPGGTYWSDLRRANGAA
                     DPFDIKLWCLGNELDGPWQIGHKTAHEYGRLAQETAKAMKLVDDSIELVAVGSSSRGM
                     PTFGTWEHTVLTHAYDEVDYISMHAYYQEHDGDAESFLAEAVEMDAFIDGVVSTIDAV
                     KAAGKHTKQVDISFDEWNVWYQSGLDTEDQPHQVSKTWREHPRLIEDTYSVTDAVVVG
                     TFLNSLLRHGDRVRIANQAQLVNVIAPIRSEEGGAAWRQTSFWPFERMARLARGRILR
                     LAVSASQIGTRRYGDVDSVDAAATWDEESGRIVVFVANRSLTDANDLTVDLRGFGDLR
                     VLKAETLTIPDGGDRHTANLEATPDAVHMVPLQADVVDGAVRTALPALSWSVIELG"
     gene            complement(4250..5167)
                     /locus_tag="VN041_18645"
     CDS             complement(4250..5167)
                     /locus_tag="VN041_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU31106.1"
                     /translation="MSTATLSPTEAVVVPSGRSARSRRPRLPGQKPFAPLRVVAFVIL
                     ALLAIGWLLPFLWAVATGFKTETDAASGDPSWIGASGPTLDAFGAILSQGNVYLWAWN
                     SLLTSVAITVITVGISALAAYAFSRLDFRGRKWLFVAIIAAIVVPPQVLIIPLFYEML
                     AFNLVDTYWGLILPQVVAPAMVFILKKFFDAVPIELEDAARVDGASRLRVFWSIILPL
                     SRPILASVAIFVFIGAWNNFLWPFLVINDTTLMTLPVGLQTVISAYGVQYAQVMAQAV
                     LAALPLIVVFLVFQKQIVKGVATSGFGGQ"
     gene            complement(5167..6111)
                     /locus_tag="VN041_18650"
     CDS             complement(5167..6111)
                     /locus_tag="VN041_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU31107.1"
                     /translation="MMTTTASTRAVVTGRKSSDPFRPAGPRRREQLVSWAFLAPFLIA
                     FALFLVWPILHGIYLSFTDQSLTGAGGDLVGFANYAEALSDPVMWQSLWNTVWFTLLS
                     TVPLVALSLIMAALVDRGLPGQWLWRLSFFMPYLLASTVISQIWVWIFNPQIGAANNI
                     LKALGLQPLAWLQNPDTNMLSIVIATVWWTVGFNFLLYLAAMQNIPPQQYEAASLDGA
                     GAWRQFWSITLPQLGPATVLILILQILASLKLFDQAYQMLGGVASDTTRSIVQYIFES
                     GFVNYRFGYSAAISYVFFALIIIIGVAQALVTRRRKVQ"
     gene            complement(6108..7442)
                     /locus_tag="VN041_18655"
     CDS             complement(6108..7442)
                     /locus_tag="VN041_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU31108.1"
                     /translation="MTPPLDLSRRQFLTAASLTAGAALLAGCAPGTASGSTTQTLQFW
                     HLLSGGDGVTMSQLLDGVNKAQSAYRIRPTVLAWGTPYYTKLAMAGAGGRAPDVAIMH
                     ATRVPGWAPGGLLDDWDLDRLAELGVDESTFPEPIWKKGIVGGKRFSIALDAHPFVLM
                     YNTDICDAAGELDSDGQLKKTSSPEEFLDQLRAVGGSAKGHALSYGYLGDGAQMWRLF
                     YGLYAQHGLAIELPAGGQMGIDKDAAVETLAFMKTLLDGEIAAAQNDYGTAVAEFATG
                     KSGMLMTGVWELRTMQNAKLPFDATMIPTLYGTPAAYADSHSFVLPHQAHADEHRRDL
                     TYRFVSDMLKGSFGWAEAGHIPAYLPVTESPDYAKLLPQAHYADAAQHVVYDPEAWFT
                     GSGSNFQGEFGAAVQGVLLSGDDPGAAIDRFIRRVNTLLAQPNPADPEGKWK"
     gene            complement(7525..>8280)
                     /locus_tag="VN041_18660"
     CDS             complement(7525..>8280)
                     /locus_tag="VN041_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWU31109.1"
                     /translation="AVQFEETALSPEREGELLARARTRQVDGLILNPVRLEDSAIPLA
                     DDLPPLVVIGEVEQSRADHVGIDSRLAAADATRHVIAQGARRIAVIGGATGQSTATAT
                     SRLRLQGVHDALQEAGIPRDPALEADRLPWSMVSGADAMQELLDRRVAFDAVVAFTDS
                     LAFGALHTLAARGIRVPDDVLLTGFDDVELSAFTTPSLTTIAFDRDALASAALSLLTA
                     RMDDRAMPPRAVTLPHRLHPRGSTAGSPGGYRP"
BASE COUNT         1288 a         2775 c         2934 g         1283 t
ORIGIN      
        1 gtcgcgcgcg ccgacggcga gcgtcgggat cttcaggtac gaacccggcg tgctgaacgc
       61 gagccgcacg gcggaggcca gcacggggtc gaaggcgagc tgcgtgggcc gcccgttccc
      121 gctcggggcc acgtgctgtg ccgaggtcgg atgccaggtg ccctgcgcgt cgcggtactc
      181 cacgctcacg tcgcggatcg tgccctcgat gttcgtgaac ccgacccggt cgaccacggt
      241 cgcggtcttc ggctgcacgg tgaacgtcgc cgagtccttc ggcgtcccgc cggtccacgt
      301 cgaccaggag gtcgacgcgc ggccgtcgca ggcgttctgc ggcggcatcg tctcccactc
      361 cgtctggtgg aagctcgcgg agatcgtcgt cccctcggcg gagcagacgt cctccacgcc
      421 gtcggcgccc cagatctgca cctccgagat cgactgccag gtgttcgtcg ccgtcgtcag
      481 gtgcagccgg atgccgctcg tctgcgggat gccggacaca tcgagtgcgg ccaccggagc
      541 cgatgcggac ggatcctgtg cgacgtcgac accggcgccg gtgggcaccc agtcgcccga
      601 ggcgtccagg tactcggcga cgatcctcga cggccaggtc gccccgctgc cgtccttgta
      661 catgtgcacg acgacctcgt cgaccttctg ggtgagcggc caggtgaacg acagccagct
      721 ggacttgggg tacacacccc cgcccaccca gtcatcccag cccttgccct tcacgtcgcc
      781 gtcggcgacc gtcgccgcga ggctctgacg gctggacgcc gatacggcag actccagcgc
      841 gaggtcgacg atcccgggag tggccacctc gatggtccgg gtgaacgtgc ggccggcggt
      901 gaactgcgcg tcggcggcga gcgtgccgac gatccgctgc gtcccgcgct cggcgaacga
      961 ggtcgcgctc caggtcacgg cggtgtccac ggtcgtgctt ccgaccttca cggggacggt
     1021 ggcgggcagg gcatccgaga agtcctcgcc gggctccacg gtcttgggca gcccgtcatc
     1081 gaccacactc cagatctcgg tgccgcggcc gtgcgcgtcg aagtacgagg cgtcccaacc
     1141 ggcgccccag cgcgccgggt cctcggtggc cgggttgcgc atctcggcgc gaccgttctc
     1201 gccgatcgtg atcggcagcc acacgtaggt ggagttcgcc gcgccggagt tccagcggtc
     1261 gcccatgtac acgaagcgtc cgccgcccag gtcgagcacg ttcgtcgact gggagccgaa
     1321 ggtcgaacga cgggtgtcgc cgaccgacag caggccgtcg ccgccctccg ggaccgagct
     1381 gtaggacacg ttctcgaacg ggtcgtcggc ctcgacgccg cggatccacg tgcccagaat
     1441 gctgtcggcg gtgtggtagg tctgcgggtt cggagcccag ccggtcgcac cggaggccac
     1501 cgcgtagtat cggccgccgt gggtgaacag tgcgggcgcc tcgagcatcc cgcactcctt
     1561 cacgatctgg aagtcctcgc cgcgcaccgg cgcatccgcc gtgccgtcgg cgaacaggta
     1621 cgggtagcgg ccgtcctccg agtactggtg ggcgttcgcc atgtcggtgc cggtcgtgtg
     1681 agtgacgttc gtgtacgagg cgtcgagctg cgcgatgtac agcgagcggt tctcctcgga
     1741 cgagtagacg atgtacgcgg tgccgtcggc gtcctggaag agcgtcatgt cgcgggcctg
     1801 gccgggcacg gcggccgaga tgcaggcctt gtagtccgtg cggttcggca gccggtagac
     1861 gccggtcatc cggaacggtc cggtggggct gtccgacacg gcgacgcccg ccatcgagcg
     1921 ggcgtacatg ctgccgccgg gcgaggtctg cccgtcggag tgccaccaga gcacccactt
     1981 gcctgtgctc tcgttgtgga gcaccttggg ccgctcgaag atggtggtgt agtcggcggc
     2041 atccgccgtg ttcaggtggt agttcagctc ggcgatgcgg tcggtgcggg gctgaccgga
     2101 ctcgtcgacg gtgtcgtaca gcgcgtcgaa gtagtcgtcc tccagctccg cggcgctgga
     2161 gacggagcgc aggacgatgc cctcgtcggt ccagttgagg gtgtcgtacg acttgtaggc
     2221 gtgcacgccg gggctgccgt agtagccgct gctgcggtcc tcgccgtacc agtagtacac
     2281 cgtgcggccc tgctcagagc ccgcgaccac gtcgccgccc tcgacgccca gctcctgcgc
     2341 cgagacgact gcgccgccgt gtgcctggat gtgcctgccg ttgtcgtcga accaggaagg
     2401 ctggaagtag tcgggcgcgt tcgcgccggg gtcgtggatc ggctgatacg ccgtgtacgc
     2461 gaccgggtct ccgaccggca cgtcggcgtg ggccgggccg gcgggggaga gggtcaatgc
     2521 ggtggcggcg cacagcgccg cggcgatgcc cttcgttctg ttcatgtgca ctccttcgtg
     2581 actcatggtc ttcgcctgca atgttcacgt aaacattgtg acgttaaaaa ccatgcaatc
     2641 agagaagcgg ggagttgcgc aagcatctcc ccgcttctct gctcgaagcc ccgagcgcgt
     2701 tcagcccagt tcgatcaccg accacgacag cgccggcagc gccgtgcgca ccgcgccgtc
     2761 gacgacatcg gcctgcagcg gcaccatgtg cacggcatcc ggagtcgcct cgaggttcgc
     2821 cgtgtgccga tcacccccat cggggatcgt gagcgtctcg gccttcagca cccgcaggtc
     2881 cccgaagccg cgcaggtcca cggtcaggtc gttcgcatcc gtgagcgaac ggttcgcgac
     2941 gaacaccacg atgcgcccgg actcctcgtc ccaggtcgcc gcggcgtcga cggagtcgac
     3001 atcgccgtag cgcctggtcc cgatctgcga tgccgacacc gcgaggcgca ggatgcggcc
     3061 gcgtgcgagc cgggccatgc gctcgaacgg ccagaagctg gtctgccgcc acgcggcgcc
     3121 gccctcctcg gagcggatcg gagcgatcac gttcaccagc tgcgcctggt tggcgatccg
     3181 cacgcggtcg ccgtgccgca gcaggctgtt caggaaggtg ccgacgacca cggcatccgt
     3241 caccgagtag gtgtcctcga tcaggcgggg gtgctcgcgc cacgtcttcg acacctgatg
     3301 cggctgatcc tcggtgtcga gccccgactg gtaccagaca ttccactcgt cgaacgagat
     3361 gtccacctgc ttggtgtgct tgcccgcggc cttcaccgcg tcgatcgtcg acacgacgcc
     3421 gtcgatgaag gcatccatct ccaccgcctc ggccaggaac gactcggcgt cgccgtcgtg
     3481 ctcctggtag taggcgtgca tcgagatgta gtccacctcg tcgtaggcgt gcgtcagcac
     3541 ggtgtgctcc caggtgccga acgtgggcat cccgcggctc gacgacccca ctgcgacgag
     3601 ctcgatcgag tcgtcgacga gcttcatcgc cttcgcggtc tcctgcgcga gccggccgta
     3661 ctcgtgcgcg gtcttgtggc cgatctgcca cggaccgtcc agctcgttgc ccaggcacca
     3721 cagcttgatg tcgaaggggt cggcggcgcc gttcgcgcgc cgcaggtccg accagtacgt
     3781 gccgccgggg tggttggcgt actccaccag cgctcgagcc tcctccaccc cgcgcgtgcc
     3841 gaggttgatc gcctccatca cctcgacatc cgcatcccgg gcccagtcga cgaactcgtg
     3901 caggccgaag gcgttcgtct cgatcgtgtg ccacgcgccg tcgatgcgca ccggacggtc
     3961 ggcgaccgga ccgaccccgt cctcccagcg gtagcccgag acgaagttgc cgccggggta
     4021 gcgcaccacc gtcgggccca tctcgcgcac cagctcgagc acgtcgcggc ggaagccgcg
     4081 ctcgtcggcc tgcggatgcc ccggctcgaa gatgcccgtg tacacgcagc gtcccatgtg
     4141 ctcgacgaac gagccgaaca ggcgtcgcgg gacgtcggcg atggtgaact cgcggtcgat
     4201 ggtgatgcgg gacgaagaca ttgctctcct tgtgaggggt gggtggatgc tactgaccgc
     4261 cgaagccgct ggtcgcgact cccttgacga tctgcttctg gaagacgagg aagacgacga
     4321 tcagcggcag ggcggccagc acggcctgcg ccatgacctg cgcgtactgc accccgtagg
     4381 cgctgatcac ggtctgaagc ccgaccggaa gggtcatcag cgtggtgtcg ttgatgacca
     4441 ggaacggcca gaggaagttg ttccacgcgc cgatgaagac gaagatcgcg acggatgcca
     4501 ggatcggccg cgacagcggc aggatgatcg accagaacac gcgcagccgg ctcgcgccgt
     4561 ccacgcgggc ggcgtcctcg agctcgatcg gcacggcgtc gaagaacttc ttcaggatga
     4621 aaaccatcgc cggtgcgacc acctgcggca ggatcagccc ccagtaggtg tcgaccaggt
     4681 tgaacgcgag catctcgtag aacagcggga tgatcagcac ctgcggcggc acgacgatcg
     4741 cggcgatgat ggcgacgaac agccacttcc ggccgcggaa gtcgagccgc gagaaggcgt
     4801 acgccgccag tgccgagatc ccgaccgtga tcacggtgat ggccaccgag gtgagcaggc
     4861 tgttccacgc ccacaggtac acgttcccct gcgacaggat cgcgccgaac gcgtcgaggg
     4921 tggggccgga cgcgccgatc caggacgggt cgccggaggc ggcatccgtc tcggtcttga
     4981 atccggtcgc caccgcccac aggaacggca gcagccagcc gatcgcgagc agcgcgagga
     5041 tcacgaaggc caccacccgc agcggcgcga acggcttctg gccgggcagg cgcggacggc
     5101 gggagcgtgc ggagcgcccc gacgggacga cgacggcctc ggtcggggag agcgttgcgg
     5161 tggacatcac tgcaccttcc tgcggcgggt cacaagggcc tgggcgacgc cgatgatgat
     5221 gatcagagcg aagaacacat aggagatggc ggccgagtag ccgaaccggt agttcacgaa
     5281 accggactcg aagatgtact gcacgatcga gcgggtggtg tcgctcgcga cgccgccgag
     5341 catctggtac gcctggtcga acagcttcag ggatgccagg atctgcagga tcaggatcag
     5401 cacggtggcc gggcccagtt gcggcagcgt gatcgaccag aactggcgcc aggctcccgc
     5461 gccgtccagt gacgccgcct cgtactgctg cggcgggatg ttctgcatcg cggccaggta
     5521 cagcaggaag ttgaaaccga cggtccacca gaccgtggcg atcacgatcg agagcatgtt
     5581 ggtgtcgggg ttctgcagcc aggcgagcgg ctgcaggccg agggccttca ggatgttgtt
     5641 ggcggcgccg atctgcgggt tgaagatcca cacccagatc tgcgagatga ccgtggaggc
     5701 cagcaggtac ggcatgaaga acgacagccg ccacagccac tggcccggca ggccgcggtc
     5761 gacgagcgcc gccatgatca gcgagagcgc cacgagcggc acggtcgaga gcagcgtgaa
     5821 ccacacggtg ttccacagcg actgccacat gaccgggtcg ctcagcgcct cggcgtagtt
     5881 ggcgaagccc accagatctc cgccggcgcc ggtgagcgac tggtcggtga agctcaggta
     5941 gatgccgtgc aggatcggcc agaccaggaa gagtgcgaac gcgatgagga acggggcgag
     6001 gaacgcccag ctgacgagct gctcgcgccg ccgcggaccc gccggacgga acgggtcgga
     6061 gctcttgcgg ccggtgacga ccgcgcgggt gctggctgtg gtggtcatca cttccacttc
     6121 ccttcggggt cggcgggatt cggctgggcg agcagggtgt tcacgcgccg gatgaaccgg
     6181 tcgatcgcgg cgccggggtc gtcgccgctg agcagcacgc cctgcacggc cgccccgaac
     6241 tcgccctgga agttcgatcc ggatccggtg aaccaggcct cggggtcgta caccacgtgc
     6301 tgcgcggcgt cggcgtagtg cgcctggggg agcagcttcg cgtagtcggg ggactcggtg
     6361 acgggcaggt acgccgggat gtgaccagcc tcggcccagc cgaacgagcc cttgagcatg
     6421 tcggagacga accggtacgt caggtcgcgg cggtgctcgt cggcgtgcgc ctgatggggg
     6481 agcacgaagg agtgcgagtc cgcgtaggcg gcgggagtgc cgtagagggt ggggatcatc
     6541 gtcgcatcga aggggagctt cgcgttctgc atggtgcgca gctcccagac gccggtcatc
     6601 agcatcccgc tcttgcccgt cgcgaactcg gcgaccgcag tgccgtagtc gttctgcgcg
     6661 gcggcgatct cgccgtcgag cagggtcttc atgaacgcga gcgtctcgac ggcggcatcc
     6721 ttgtcgatgc ccatctgccc gccggccggc agctcgatcg ccagcccgtg ctgcgcgtag
     6781 agcccgtaga acagacgcca catctgggcg ccgtctccga ggtagccata cgagagcgcg
     6841 tgacccttcg ccgacccgcc gaccgcgcgc agctgatcga ggaactcctc gggcgaggag
     6901 gtcttcttca gctgcccgtc ggagtcgagt tcgcccgccg cgtcgcagat gtcggtgttg
     6961 tacatgagca cgaaggggtg ggcgtccagc gcgatgctga accgcttgcc gccgacgatg
     7021 cccttcttcc agatcggctc ggggaaggtg gactcgtcca cgccgagctc ggccaggcgg
     7081 tccagatccc agtcgtcgag cagcccgccc ggcgcccagc cgggaacgcg cgtggcgtgc
     7141 atgatcgcga cgtcgggggc gcgtccgcct gcgcccgcca tggcgagctt cgtgtagtac
     7201 ggggtgcccc aggcgagcac ggtggggcgg atgcggtagg cgctctgcgc cttgttgacg
     7261 ccgtccagca gctgggacat ggtcaccccg tcgccgccgg agagcagatg ccagaactgc
     7321 agtgtctggg tggtggagcc ggatgcagtg ccaggcgcgc agccggccag gagggcggcg
     7381 ccggcggtga gggaggcggc ggtgaggaac tgccttcgag agagatctag cgggggtgtc
     7441 atgaatcact ccttcgtgaa cggcaccatc attacatcgt ggtaatcaat gcgcaagagc
     7501 ctctgcgcac gattgtcggc ggcgtcaggg acggtatccg ccgggactgc ccgcggtgct
     7561 gccccggggg tgcagccggt gcggcagcgt gaccgcccgg ggaggcatcg cgcgatcgtc
     7621 catgcgcgcc gtcagcagcg agagcgccgc gctcgccagc gcgtcccgat cgaatgcgat
     7681 cgtggtgagc gacggggtgg tgaacgctga gagctccacg tcgtcgaagc cggtcagcag
     7741 cacgtcgtcg ggaacgcgga tgccgcgtgc cgccagcgta tgcagggcgc cgaacgccaa
     7801 cgagtcggtg aaggcgacca cggcatcgaa ggcgacccgc cggtccagca gctcctgcat
     7861 cgcgtcggct ccgctcacca tcgaccacgg cagccgatcc gcctcgagcg caggatcgcg
     7921 ggggatcccc gcctcctgga gtgcatcgtg cacgccctgc agacgcaggc ggctggtggc
     7981 ggtggcggtg ctctggccgg tcgccccgcc gatcacggcg atccgccgcg caccctgggc
     8041 gatcacgtgc cgggtcgcat cggccgccgc cagccggctg tcgatgccga cgtggtccgc
     8101 ccggctctgc tccacctcgc cgatcaccac gagcggcggg aggtcgtccg ccagagggat
     8161 cgcgctgtcc tccaatcgca ccgggttgag gatgaggccg tccacctggc gcgtgcgtgc
     8221 gcgtgcgagc agctcgccct cgcgctcggg ggagagcgcg gtctcctcga actgcacggc
//
LOCUS       DATMSX010000576         7152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_18158,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000576 DATMSX010000000
VERSION     DATMSX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18158"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VN041_18665"
     CDS             complement(<1..654)
                     /locus_tag="VN041_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWU31110.1"
                     /translation="MQHRDGGAPDVEVVLLGDLNVDLHLDIPVYPGPGGDGVATRQRM
                     GFGGSAANTAVMLSRLGVRSAMIACVGDDAWGAQAIAGLAAEGVDADLVRRCATEPTS
                     LNVITVTPDGERTMFAYRGASAELGPEAVPADLRGARHLHISGYALLADPQRSAAIAA
                     AQHARRAGMTVSLDVPVDPVNAVPEVLLGFLASVDVVIVGAGDARRLTAEPDDERAAD
                     "
     gene            complement(659..1849)
                     /locus_tag="VN041_18670"
     CDS             complement(659..1849)
                     /locus_tag="VN041_18670"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWU31111.1"
                     /translation="MDNPQELSLASRAVFYSPEESTQSISYPIFMSANYQYDGDAYDQ
                     VVEGARRSVNIYSRCGNPNEYKLDDQMALISGATDALAVASGMAAVSHAVLGLLRVGD
                     HIVVDLTTYSSTHEFFDHRIQDFGIEVTFVDSADVEAVRAAIRPETKAVYVETIANPT
                     MKVAPLRAITELAHERGIVVICDNTFASPIVCRPHEHGVDIVLESATKFIGGHNDAVG
                     GIISINSEILPADWLEQIRWNTLTKLGGALSPFNAWLLLRGIQTLPLRVERMTENAGR
                     LVEWLEAHPKVRRVYYPGHPSHPQYDAAREQLDSPGAMLAFSVASEEEAAQVCKSLKL
                     ASFAASLGGVRTVTQLPATMAFLDIPEEERQQMGVTQGMIRVSAGIEKIDDLIADFAQ
                     ALGD"
     gene            complement(1896..2810)
                     /locus_tag="VN041_18675"
     CDS             complement(1896..2810)
                     /locus_tag="VN041_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU31112.1"
                     /translation="MSQATMAVTMPDETLTPRRQRRREREQLPRTRLSAGVVTVIALL
                     GVHLVVVLFPFVWMIYSSFKTNQQFQQSVWSLPSSLQWDNYATALADGSLALYAGNSL
                     IVTLVSVLITVAIATAAGYAFVVYRTRWMPLVEIVMLVAMAIPAYIALVPLVAIIREM
                     GMLDTLPGVILPTVAFNLPISVLIMRGFFATVPRDLIEAARIDGASELSTFARVMVPI
                     AKPAMFTTAIVNVIWVWNDFLFPLVILNSPERKTLPLGLTDFVGERTTNYPVLLAAIL
                     LAALGSFLVYLVFQRHVIGGLTSGALKQ"
     gene            complement(2807..3751)
                     /locus_tag="VN041_18680"
     CDS             complement(2807..3751)
                     /locus_tag="VN041_18680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU31113.1"
                     /translation="MTSALSDRARRGGLHRAKRRESLIALLFLVPALAFLAIFVAYPL
                     ANAGMLSFFKWDGFSPREWVGIDNFVRLAADGVFWSSLRNNVLIAVAAIVFQVGLGMV
                     IAYWLVRVIPRFKRAAMFLYVIPVVISEICIGLLWQFVYNPYFGLLNGFLRLIGLGDL
                     AKGWLGDKTLAMPAVLVVMNLTYLGLYILLFVAAFQDVDESVYEAASLDGAGHFRTFF
                     GISAPMIASNVQATVLLAVVTSFKTFSLVFVMTRGGPSNATDVISTYLFKTGFGNFEA
                     GYASAIGIAQLLITVIVGVFVLTATRPRRPKTIKEVAR"
     gene            complement(3789..5078)
                     /locus_tag="VN041_18685"
     CDS             complement(3789..5078)
                     /locus_tag="VN041_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU31114.1"
                     /translation="MNRHLRKAAAAALAVGALVALSGCVGSGSAPAAGGGADSLTIWH
                     AYAGQADKVEFMKWAMDGFKKEHPEAKIKEVSAEQSSYKTKLQTAMSTGDAPDVFYTL
                     PGGFLDAFVKSGQVADLDDALAKDDWGDSFIPAAMDSVTFDDSTYAVPIDIDAAVVWY
                     NKALFEKKGWTTPKTWDEFVQLSEKIAADGIVPVALGNKDSWPATFWFQYGQMRTEGS
                     GLVTDFLNGDGKIALDAKGAEPLQTLAQKKLLPTGANGMSDQEANLLFLNGQAAMVLN
                     GTWQIGMSADAPAGFELGYFPFPTVADGKGDQTDTLAGVAAAFAISEKAKDKKLATEF
                     LQYMTSPDVMKKYVELRKTMVTLKGATTPDVAGPVLSGIVSDIIEPAGHLDPFYDTAL
                     PPKATTVYYSTLQGLIEGSVDAQQAVDAINEAISAGK"
     gene            5196..6047
                     /locus_tag="VN041_18690"
     CDS             5196..6047
                     /locus_tag="VN041_18690"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HWU31115.1"
                     /translation="MASHERAGAMTLLRAALPGLSTTERRVADWILTRPEHLLEASMI
                     DVAKACEVSDTTVLRMCRNAGFAGYTDLKLALARDLATPMQLIHDDVADGDDPMTIAR
                     KVFAHAALSLQDTADVIAQDDFTAALELLETSRSVLVGGVGTSGVVGQSFYQRCRRLG
                     ISCDAPQDSQVQNIHAALLDPGDLVVVISYSGETRSVVSMAEGARRAGAKVLAVTGNR
                     ESHLAKVADVVLQSVSHETRNEPLAARLCQLALLDALCVAYSHRHLADVLSRDQRAGQ
                     SIVESSF"
     gene            6082..6759
                     /locus_tag="VN041_18695"
     CDS             6082..6759
                     /locus_tag="VN041_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWU31116.1"
                     /translation="MIVQIYTAQSADEAVALAERGVDHVGLTPTTVGLPGQISEAVAA
                     EAVAALRGRARSVALSVETELAEIARMAAAVRPDILHLCGEIGAVTPDDVRELRGMLP
                     AGMEIMQAIAVGAEDDIAMAREYAQVADYLILDSYTTDVQGVGAAGVTHDWSRSAEIV
                     ASVDIPVILAGGLSAANVAESIAAVRPWGVDSLTLTNRPLGDGRFEKDLDAVAAFVAG
                     ATAVPAV"
     gene            complement(6826..>7152)
                     /locus_tag="VN041_18700"
     CDS             complement(6826..>7152)
                     /locus_tag="VN041_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HWU31117.1"
                     /translation="LAALGRQVDVVVVTSPGLLFDAVQARGGQGSADAWILDQVLPAE
                     RAVPMVTVLDGHPHALAFLAGIQGVPARHLGVSRFGQAGDLADVYRYHGIDEDSMVRS
                     VLDLAG"
BASE COUNT         1178 a         2414 c         2436 g         1124 t
ORIGIN      
        1 atccgccgcc cgctcgtcat cgggctccgc cgtgagccgg cgcgcgtcgc ctgctccgac
       61 gatcacgacg tcgaccgaag cgaggaagcc cagcagcacc tcgggaaccg cgttgacggg
      121 gtcgacgggc acgtccagcg ataccgtcat ccccgcgcga cgggcgtgct gcgccgccgc
      181 gatcgcggcg gagcgctgcg ggtccgcgag cagggcatag cccgagatgt gcaggtgcct
      241 cgcgccgcgg agatccgccg gcaccgcctc cggtccgagc tcggcgctcg cgccgcggta
      301 cgcgaacatc gtccgctcgc cgtccggggt gaccgtgatc acgttcaggg aggtgggctc
      361 ggtggcgcag cgacgcacga ggtccgcatc cacgccttcc gccgccaatc cggcgatcgc
      421 ctgcgcaccc caggcgtcgt cgccgacgca ggcgatcatc gccgagcgga cgccgagcct
      481 cgacagcatc accgcggtgt tcgcggccga accgccgaag cccatccgct gccgggtggc
      541 gacgccgtcg ccgcccggcc ccggatagac cgggatgtcg aggtgcaggt cgacattgag
      601 gtcgcccaga agcacgacct ccacgtccgg cgccccgcca tccctgtgct gcacgtcgtc
      661 agtctcccag tgcctgcgcg aagtcggcga tgaggtcgtc gatcttctcg atgcccgccg
      721 acacgcggat catgccctgg gtgacgccca tctgctgacg ctcctcctcg gggatgtcga
      781 ggaaggccat ggtcgccggc agctgcgtca ccgtgcgcac gccgccgagg ctggctgcga
      841 acgaggcgag cttcaggctc ttgcacacct gcgccgcctc ctcctcgctg gcgacgctga
      901 acgccagcat ggcgccggga gaatcgagct gctcccgtgc cgcgtcgtac tgcgggtgcg
      961 acggatgacc cgggtagtag acgcgccgca ccttggggtg cgcctcaagc cactccacca
     1021 ggcggccggc gttctcggtc atccgctcga cgcgcagcgg cagggtctgg atgccgcgca
     1081 gcagcagcca ggcgttgaac ggcgagaggg caccaccgag cttggtcagc gtgttccagc
     1141 ggatctgctc cagccagtcg gcgggaagga tctcggagtt gatgctgatg atgccgccga
     1201 cggcgtcgtt gtgcccgccg atgaacttcg tggcactctc gagcacgatg tcgacgccgt
     1261 gctcgtgcgg acggcagacg atcgggctcg cgaaggtgtt gtcgcagatc acgacgatgc
     1321 cgcgctcgtg cgcgagctcg gtgatcgccc ggagaggggc caccttcatg gtcgggttcg
     1381 cgatggtctc gacgtagacc gccttggtct cggggcggat cgccgcgcgc accgcctcca
     1441 catcggcgga gtccacgaag gtcacctcga tgccgaagtc ctggatgcgg tggtcgaaga
     1501 actcgtgcgt tgagctgtac gtggtcaggt ccacgacgat gtgatccccc acccgcagca
     1561 ggccgagcac ggcatgcgac accgcggcca tcccggacgc caccgccagc gcgtccgtcg
     1621 cgccggagat cagcgccatc tggtcgtcga gcttgtactc gttggggttg ccgcagcggc
     1681 tgtagatgtt cacgctgcgc cgggcgccct cgaccacctg atcgtaggca tcgccgtcgt
     1741 actggtagtt ggccgacatg aagatcgggt agctgatcga ctgcgtgctc tcctcgggcg
     1801 agtagaacac ggcacggctg gcgaggctca gttcctgcgg gttgtccaca gaaggtcctt
     1861 tctgttcgag tgctgatgcg gcgattgccg tcagatcact gtttgagcgc gccgctggtc
     1921 agcccgccga tgacgtggcg ctggaacacc aggtacacca ggaacgagcc gagggcggcg
     1981 aggaggatcg cggcgagcag caccgggtag ttcgtggtgc gctccccgac gaagtcggtg
     2041 agtccgagcg gcagggtctt gcgctcgggg ctgttgagga tgacgagcgg gaagaggaag
     2101 tcgttccaca cccagatgac gttgacgatc gcggtggtga acatggccgg cttcgcgatc
     2161 ggcaccatca cccgcgcgaa cgtggacagc tcggatgccc cgtcgatgcg cgccgcctcg
     2221 atcaggtcgc gcggaacagt ggcgaagaac ccgcgcatga tcagcaccga gatcggcagg
     2281 ttgaacgcca cggtcggcag gatcaccccg gggagcgtgt ccaacatccc catctcgcgg
     2341 atgatcgcca ccagcggcac gagggcgatg taggccggga tcgccatcgc gaccagcatc
     2401 acgatctcga cgagcggcat ccagcgcgtg cggtagacca cgaaggcgta cccggccgcc
     2461 gtggcgatcg cgaccgtgat gagcacggag acgagcgtga cgatgagcga gttcccggcg
     2521 tacagggcga gggatccgtc ggcgagcgcc gtggcgtagt tgtcccactg caggctggac
     2581 ggcagcgacc agaccgactg ctggaactgc tggttcgtct tgaacgagct gtagatcatc
     2641 cagacgaacg ggaacagcac cacgaccaga tgcacgccca gcagcgcgat gacggtgaca
     2701 acgcccgcgc tgagcctggt gcgcggcagc tgctcgcgct cgcgtctgcg ctgccgacgc
     2761 ggcgtgagcg tctcgtcggg catggtgacg gccatggtcg cctggctcat cgtgcgacct
     2821 ccttgatcgt cttgggccgg cggggccgcg tggcggtgag cacgaacacg ccgacgatca
     2881 ccgtgatcag cagctgcgcg atgccgatcg ccgacgcgta gccggcctcg aagttgccga
     2941 agccggtctt gaacaggtac gtcgagatga cgtcggtcgc gttgctcggt ccgcccctcg
     3001 tcatcacgaa cacgagagag aaggtcttga acgaggtgac caccgcgaga agcacggtcg
     3061 cctgcacgtt cgaggcgatc atcggcgcgc tgatgccgaa gaaggtgcgg aagtgacccg
     3121 cgccgtccag ggacgcggcc tcgtacaccg actcgtcgac gtcctggaac gcggcgacga
     3181 acagcaggat gtagaggccc aggtaggtga ggttcatgac cacgagcacg gcgggcatcg
     3241 cgagggtctt gtcgccgagc catcccttcg cgaggtcgcc caggccgatg aggcgcagga
     3301 acccgttgag caggccgaag taggggttgt agacgaactg ccacagcagg ccgatgcaga
     3361 tctcgctgat cacgaccggg atgacgtaga ggaacatcgc ggcgcgtttg aaccgcggga
     3421 tgacccgcac cagccagtac gcgatcacca tgccgagccc gacctggaac acgatcgccg
     3481 cgaccgcgat gagcacgttg ttcctgagcg acgaccagaa caccccgtcc gccgcgagcc
     3541 tgacgaagtt gtcgattccg acccactccc gtggtgagaa gccatcccac ttgaagaacg
     3601 acagcatgcc cgcgttggcg agggggtagg cgacgaagat cgccaggaac gccagggcgg
     3661 ggacgaggaa cagcagggcg atcagcgact cacggcgttt ggcgcgatgc agcccaccgc
     3721 gccgggcgcg gtccgagaga gcgctcgtca tgagcgccct ctcggaccgg atctcgcagg
     3781 gcacccgctc acttccccgc gctgatcgct tcgttgatcg cgtcgacggc ctgctgggcg
     3841 tcgaccgagc cctcgatcag cccctgaagc gtcgagtagt agacggtcgt ggccttcggc
     3901 ggcagcgcgg tgtcgtagaa cggatcgagg tgccctgccg gctcgatgat gtcggacacg
     3961 atgcccgaga gcacggggcc cgcgacatcc ggcgtggtcg cgcccttcag cgtcaccatg
     4021 gtcttgcgca gctcgacgta cttcttcatc acgtcggggc tcgtcatgta ttgcaggaac
     4081 tcggtggcga gcttcttgtc cttcgccttc tcggagatcg cgaaggcggc ggcgacgccc
     4141 gcgagggtgt cggtctggtc gcccttgccg tccgcgacgg tcgggaacgg gaagtacccg
     4201 agctcgaagc ctgccggcgc gtcggccgac atgccgatct gccacgttcc gttgagcacc
     4261 atggccgcct gcccgttcag gaacagcagg ttcgcctcct ggtcggacat gccgttcgca
     4321 ccggtcggca ggagcttctt ctgcgcgagc gtctgcaggg gctcggcacc cttcgcgtct
     4381 agcgcgatct tgccgtcgcc gttcaggaag tcggtgacca gaccgctgcc ctccgtgcgc
     4441 atctgcccgt actggaacca gaaggtcgcc ggccagctgt ccttgttgcc gagcgccacg
     4501 gggacgatgc cgtcggctgc gatcttctcg ctgagctgca cgaactcatc ccacgtcttc
     4561 ggcgtcgtcc accccttctt ctcgaagagc gccttgttgt accagacgac ggcggcgtcg
     4621 atgtcgatcg gcaccgcgta ggtggagtcg tcgaaggtga ccgagtccat cgcggcagga
     4681 atgaagctgt cgccccagtc atccttggcg agagcatcgt cgaggtcggc gacctggccg
     4741 ctcttgacga aggcgtcgag gaacccaccc ggcagggtgt agaagacatc gggcgcatcg
     4801 cccgtggaca tcgcggtctg cagcttggtc ttgtacgacg actgctcggc ggagacctcc
     4861 ttgatcttcg cctccggatg ctccttcttg aagccgtcca tcgcccactt catgaactcg
     4921 accttgtcgg cctgcccggc ataggcgtgc cagatggtca ggctgtcggc gccgcctccc
     4981 gcggcggggg ccgatccact gcccacgcaa ccggacaacg cgaccagcgc gccgacggca
     5041 agcgccgccg cggcggcctt gcggaggtga cggttcatga ggactccttc tgacgacact
     5101 gtcgggaaac gtggtatcac catatactca tcgagttgga tgtggtagca ccacattacg
     5161 atccgccggc cagatcggaa gcgaggatag gacgcatggc atcccatgag agagctggag
     5221 cgatgacgct cctgcgcgca gcccttcccg gtctttccac caccgagcgc cgcgtcgccg
     5281 actggatcct cacccggccc gagcacctgc tcgaggcgtc gatgatcgac gtcgcgaagg
     5341 catgcgaggt gagcgacacc accgtgctgc ggatgtgccg caacgccggg ttcgcgggct
     5401 acaccgacct gaagctcgcg ctcgcccggg acctcgcgac tccgatgcag cttatccacg
     5461 acgatgtcgc ggacggggac gacccgatga ccatcgcgcg gaaggtgttc gcgcacgccg
     5521 ccctctcgct gcaggacacc gcggacgtca tcgcgcagga cgacttcacc gccgcgctcg
     5581 aactgctcga gacgtcgcgc agcgtgctcg tcggcggcgt cggcacgtcg ggtgtcgtcg
     5641 gccagtcgtt ctatcagcgc tgccggcgac tcggcatcag ctgcgacgcc ccccaggact
     5701 cgcaggtgca gaacatccac gccgcactgc tcgaccccgg cgatctcgtc gtcgtgatct
     5761 cgtactccgg cgagacgcgg tcggtggtca gtatggccga gggcgccaga cgcgcggggg
     5821 cgaaggtgct cgccgtcacc ggaaaccgcg aatcgcacct cgccaaggtc gccgacgtgg
     5881 tgctgcagag cgtgtcgcac gagaccagga acgagcccct cgctgcccgt ctctgccagc
     5941 tcgcccttct ggacgccctc tgcgtcgcgt acagccaccg tcatctcgcc gatgtgctga
     6001 gcagggatca gcgcgcgggc caatccatcg tcgagagctc gttctgactc tcgatcccac
     6061 ctcacagagg agtcgtacac gatgatcgtc cagatctaca cggcgcagtc cgccgatgaa
     6121 gcggtcgcgc tcgcagagcg gggggtcgac catgtcggtc tcacgccgac gaccgtcgga
     6181 ctgcccggcc agatcagcga ggccgtcgct gccgaggcgg tggcagcact gcgcggacgc
     6241 gcacgttccg tggccctcag cgtcgagacc gaactggccg agatcgcacg catggcggcg
     6301 gcggtgcgcc ccgacatctt gcatctgtgc ggtgagatcg gcgccgtcac gccggacgac
     6361 gtgcgggagc tgcgcggaat gctgcccgcc ggcatggaga tcatgcaggc gatagcggtc
     6421 ggcgccgagg acgacatcgc gatggccagg gagtacgcgc aggtcgccga ctacctgatc
     6481 ctggactcgt acaccaccga tgtgcagggc gtgggcgccg cgggtgtgac gcacgactgg
     6541 tcgcgcagcg cggagatcgt cgcgagcgtg gatatcccgg tgatcctggc agggggcctc
     6601 tcagcggcga acgtcgccga gtcgatcgcc gccgtgcgcc cgtggggcgt ggactcgctg
     6661 acgctgacga accgcccgct cggagacggc cggttcgaga aagatctcga cgccgtggcc
     6721 gcgttcgtcg caggcgcgac cgcggtcccc gcggtctgag agcgggcgcg gtcgcgcggg
     6781 catccgccta cccggcgcgc atccagttac ccggcgagta tccgcctacc ctgctagatc
     6841 gagcacggac cgcaccatgc tgtcctcgtc gatgccgtgg taccggtaga cgtcggcgag
     6901 gtcgccggcc tggccgaacc tgctcacgcc gagatggcgg gcgggaaccc cttggatgcc
     6961 ggcgaggaac gccagcgcat gcggatgccc gtccagcacc gtcaccatcg gcacggcgcg
     7021 ctccgcgggc agcacctgat cgaggatcca tgcgtccgcg ctcccctgcc cgccgcgtgc
     7081 ctgcaccgca tcgaacagaa ggccggggct ggtgaccacg accacgtcga cctgcctgcc
     7141 gagcgcggcg ag
//
LOCUS       DATMSX010000577         8734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_113348,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000577 DATMSX010000000
VERSION     DATMSX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113348"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..942
                     /locus_tag="VN041_18705"
     CDS             7..942
                     /locus_tag="VN041_18705"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWU31118.1"
                     /translation="MDARWGRAYFGAQAAAGALWWAAVALSPGIRMLTLGDLPAGLVA
                     AVDIPLFVIASALAALGVRFAAEITASWTVLITAAMALYSTITALAGWGSLLMIAASV
                     CSVGALLLVRLNRIPSEWLLIGPFRAREATSASPRTQLLRTAGQIVVFWGVCLVVVPA
                     GIALVELRWGLRMPLPPAVTITGVVLLAAASALGICSAVTMAGRGDGTPLPVATARLL
                     VVGGPYRFVRNPMAVAGIAQGVAVGLICGSWLVVIYALCGSLVWNDLIRPWEERDLVA
                     RFGAPYEAYRDRVSCWIPRRPRRRSSGSVSDPLVY"
     gene            948..2036
                     /locus_tag="VN041_18710"
     CDS             948..2036
                     /locus_tag="VN041_18710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWU31119.1"
                     /translation="MSEHTDSASSPAPATRAEARRRADRSGGADISRALDRHDRRIGW
                     MDLLRGLSILLVVLHHSTQITAYRIGEVPGFFEFVSNFFAPFRMPMLMFLSGLLVAGS
                     LHVPAGKYVWGKVRRILWPIFVWTLVYAAMEFVAEPSQVKFMPWELGFWNTYLWFMQF
                     VFAYYIIALLMKWLPIWVLFAVPFVLMFVIPADLELLRRFFYLMPFFFLGAFIEKHWD
                     EYAKRLSVPLAAGLAIIPIGVGLYSGLIDPLWYEPLSAVPAMLGIMILVRLTAAAPDV
                     RWLAPARFVGRYSLIYYVSHYPVFAALGWLALKVGFDNPTLGLLAIFLVTVAISTGFA
                     LLGRRMPVSLLFELPRRLWPARKPASAT"
     gene            complement(2100..2459)
                     /locus_tag="VN041_18715"
     CDS             complement(2100..2459)
                     /locus_tag="VN041_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWU31120.1"
                     /translation="MAEVRFDETIHAPVRLRICGLLRQVDRMDFALLRDTLEVSDATL
                     SKHLKIIITAGFASSAKEASAERKDARRLTWISLTPAGRRAFDAHLRALQQIAAPDGV
                     PQVAARAPQLRFEHLHP"
     gene            complement(2452..2919)
                     /locus_tag="VN041_18720"
     CDS             complement(2452..2919)
                     /locus_tag="VN041_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31121.1"
                     /translation="MERNSDATRRDAAIALSTLADDRSRLAARIRVPWVLLAALGGLA
                     ALGVVSPAIANPGDDYQPPLVVGMAVLIGVIIVGYLIQRGTGIRFRTMGVAANAALCG
                     VVATCLLLFMLSLALVSLELRWTLVLTGAAAFVVTTGLARVAYRSAAKRLRRG"
     gene            3077..4123
                     /locus_tag="VN041_18725"
     CDS             3077..4123
                     /locus_tag="VN041_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWU31122.1"
                     /translation="MTITAPALAGSALHPRGPLGEAVLDALIASEPADRTSLASQADS
                     ALRCCSDIMTDDDIQFALFLLYASAYGTLDGLDAGREWDPALIAVRLALESAFEQDLR
                     ARVAMPDLPEPTADAVARALFALVAADDGPSLSRHLARRATNEQAHEFLVQRSIYTLR
                     EADPHSWAIPRLSGSPKAALVEIQSDEYGGGRPDRVHAELFAKALRGAGLDDTYGAYV
                     DDVPAITLASHNMMSMFGLNRRLIGAIVGHLAAFEMTSSIPNRLYADGLRRLGFGDDV
                     TDYFDEHVEADAVHEQIAARDLAGGLAAQHPHLLPDIMFGAAACLAIDGAVAGHMLDA
                     WERQSSSLRTGVTA"
     gene            4120..4326
                     /locus_tag="VN041_18730"
     CDS             4120..4326
                     /locus_tag="VN041_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWU31123.1"
                     /translation="MSASAPQPTITPYPDGPLLLRGDVVLQTADGEVIPRHRRTVALC
                     RCGLSAIKPFCDGTHKAAGFRTEG"
     gene            complement(4336..4893)
                     /locus_tag="VN041_18735"
     CDS             complement(4336..4893)
                     /locus_tag="VN041_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWU31124.1"
                     /translation="MGTLTFSLNVTLDGCVDHREGIADDESHAFFTCLMDEAGAMLWG
                     RVTYEMMEGYWPAVARGEEEAPPAMREWAVKLDAKPKHVVSSTRTDFPWSNSHHLSGD
                     LREGVQRLKDETPAGVLLGSGSLATELDRLDLIDEYRFLVQPMIAGHGPTLFQAGLPA
                     TRRLELISATPLRSGAVDMHYRRAR"
     gene            complement(4941..5013)
                     /locus_tag="VN041_18740"
     tRNA            complement(4941..5013)
                     /locus_tag="VN041_18740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4978..4980),aa:Val,seq:tac)
     gene            5167..7893
                     /gene="aceE"
                     /locus_tag="VN041_18745"
     CDS             5167..7893
                     /gene="aceE"
                     /locus_tag="VN041_18745"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWU31125.1"
                     /translation="MTVHDQDPYSQGPLDSDPEETGEWQQSLDELVDAKGHGRGREIM
                     LSLLKRSKDLHLGVPMVPTTDYINTIAPENEPEFGGDEEIERRYRHWIRWNAAITVHR
                     AQRPGIGVGGHISTYAGAASLYEVGFNHFFRGQNHPGGGDQIFIQGHASPGIYARSFL
                     EGRLSEADMDGFRQEKSHEGHALPSYPHPRSMPDYWQFPTVSMGLGPINAIYQAQSNK
                     YLANRGIKDTSDQHVWAFLGDGEMDEVESRGQLQVAANEGLDNLTFVINCNLQRLDGP
                     VRGNGKIIQELESFFRGAGWNVIKVVWGREWDDLLARDTDNALLNIMNATPDGDYQTY
                     KAESGAYIREHFFGKDERAAALVKDYTDEQIWQLRRGGHDYRKVFAAYKAALEHKGQP
                     TVILAKTVKGYGLGPHFEGRNATHQMKKMTLENLKTFRDTMQIPITDAQLEENPYLPP
                     YYHPGENDETIQYMLERRRALGGFIPERRTKHTAITLPDDSTYALPKKGSGTQEIATT
                     MAFVRLLKDLLRSKDFGNRIVPIIPDEARTFGIDAYFPTAKIYNPNGQHYTSVDRELL
                     LAYKESPQGQIVHVGINEAGATAAFTAAGTSYATQGEPLIPIYIFYSMFGYQRTGDAL
                     WAAGDQMARGFVIGATAGRTTLTGEGLQHADGHSPLLASTNPATVSYDPAYGYEIAHI
                     MRSGLERMYGGNHPNPDVMYYLTVYNEPLVQPAEPEDVDVDGIIRGIHRISVDSGEGL
                     KAQLFASGVGVPWALEAQELLRKDWNISADVWSVTSWGELRNDGLAADEHNFLHPDEE
                     PRTAYLTEKLQGAEGPVIAASDFMHAVQDQIRQWVPNPYYTLGADGFGFSDTRAAARR
                     FFKIDGPSMVVRTLQALADQGKIQRSIVGDAIIKYALHDVNAGSSGNAGGES"
     gene            7922..>8734
                     /locus_tag="VN041_18750"
     CDS             7922..>8734
                     /locus_tag="VN041_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HWU31126.1"
                     /translation="MDKAATLAWLRRISGDIATVTIKRLEDTLPWYADMPPARRSAVG
                     LVAQAGITSFIQWYEDPSSTPWIAADIFAAAPRELLRSVSLQQTLQLIRVTVAVTEER
                     VAGRGEDLREAILLYSRDVAFASADVYARAAEARGLWDARLEALVVDSILTGEADEEL
                     PSRIAALGWHGHGEVCVLVGTTPPQFDVDQVRRTARRLSVDVLIGVQGSRLVLVVGRA
                     RVPGKETAEEEELPFAEIAQRLEPSFGPGYVVLGPPVAALVDAGQSARAALAG"
BASE COUNT         1344 a         3082 c         2930 g         1378 t
ORIGIN      
        1 gcacggatgg acgcccgctg ggggcgcgcg tacttcggcg cgcaggcggc ggcgggcgcc
       61 ctgtggtggg ctgctgtcgc gctctccccc ggcatccgga tgctgacgct cggcgacctc
      121 cccgcgggac ttgtcgccgc agtcgacatc cccctcttcg tcatcgcgtc tgcgctcgcc
      181 gcgctgggtg tgcggttcgc cgccgagatc acggcgagct ggaccgtgct gatcacggcg
      241 gccatggccc tgtactcgac gatcacggcg ctcgccggct gggggtccct gctcatgatc
      301 gcggcatcgg tctgctcggt gggcgcgctg ctgctggtcc ggctgaaccg gatcccctcg
      361 gagtggctgc tcatcggacc gttccgcgcc cgcgaggcga catcggcgtc gccgcgaacg
      421 cagctgctgc gcacggccgg ccagatcgtg gtcttctggg gagtctgcct cgtggtcgtc
      481 ccggcaggca tcgcgctcgt cgagctccgg tgggggctgc ggatgccgtt gccgcccgcc
      541 gtcacgatca ccggcgtcgt cctcctcgca gccgcctcgg cactcggcat ctgttcggcg
      601 gtcacgatgg cggggcgcgg tgacggcacc ccgctgccgg tcgcgaccgc ccggctgctc
      661 gtggtgggcg gcccgtaccg gttcgtgcgc aatccgatgg cggtggcggg gatcgcccag
      721 ggggtggctg tcggcctgat ctgcggctcg tggctcgtcg tgatctacgc gctgtgcggc
      781 tcactcgtgt ggaacgacct catccggccg tgggaggagc gggatctcgt cgcccggttc
      841 ggcgccccgt acgaggcgta tcgtgaccgc gtgtcctgct ggatcccgcg gcgcccacgc
      901 cgccgttcct ccggttccgt cagcgacccc ctcgtatact gaccgggatg tccgagcaca
      961 ccgattccgc gtcctccccg gcccctgcga cccgcgccga ggcgcgccgt cgcgccgacc
     1021 gcagcggcgg agccgacatc agtcgcgctc tggaccggca cgatcgccgg atcggttgga
     1081 tggacctgct gcgcggactc tcgatcctgc tggtggtcct gcaccacagc acgcagatca
     1141 ccgcgtaccg catcggcgag gtgccgggct tcttcgagtt cgtcagcaac ttcttcgcgc
     1201 cgttccggat gccgatgctg atgttcctct ccggactgct ggtcgcgggc tccctgcacg
     1261 tgcccgccgg caagtacgtg tggggcaagg tgcgccgcat cctgtggccc atcttcgtgt
     1321 ggaccctggt gtacgcggcg atggagttcg tggcggagcc gtcccaggtg aagttcatgc
     1381 cgtgggagct gggcttctgg aacacctacc tgtggttcat gcagttcgtc ttcgcgtact
     1441 acatcatcgc gctgctcatg aagtggctgc cgatctgggt cctgttcgcg gtgccgttcg
     1501 tgctgatgtt cgtgatcccc gccgacctcg agctgctgcg ccggttcttc tatctgatgc
     1561 cgttcttctt cctgggcgcg ttcatcgaga agcactggga cgaatacgcg aagcggctga
     1621 gcgtgccgct cgcggcggga ctcgcgatca tccccatcgg cgtcggcctg tactcgggcc
     1681 tgatcgaccc gctctggtac gagcccctct cggcggtgcc cgcgatgctg gggatcatga
     1741 tcctggtgag gctcaccgcg gccgcgcctg atgtccgctg gctggcgccc gcccggttcg
     1801 tgggcaggta ctccctgatc tactacgtct cgcactaccc cgtcttcgcc gccctcggct
     1861 ggctggcgct caaggtcggg ttcgacaatc cgacgctggg tctgctggcc atcttcctgg
     1921 tgaccgtggc gatctcgacg ggcttcgccc tgctggggcg ccggatgccg gtgagtctgc
     1981 tcttcgagct tccgcgccgc ctctggccgg cgaggaaacc cgcatcggcg acgtaggaac
     2041 ggcacgcctc gcgcgagtgg atccgagggg cactgccctg agcccggcgt gcggcgaggt
     2101 cagggatgca ggtgctcgaa gcggagctgc ggggcgcgcg cggccacctg tggaacgcca
     2161 tccggcgcgg cgatctgctg cagggcacgc agatgtgcat cgaacgcccg tcgccccgcc
     2221 ggggtgagcg atatccaggt cagacgtcgc gcgtccttcc gctctgcgga tgcttccttc
     2281 gccgaagacg cgaagcctgc cgtgatgatg atcttcaggt gcttggacag ggtcgcgtcg
     2341 gagacctcga gggtgtcgcg cagcagcgcg aagtccatcc ggtcgacctg ccggagcagt
     2401 ccgcagatcc gcagccgcac aggtgcgtgg atcgtctcgt cgaagcggac ttcagccacg
     2461 tcgcagtctc ttcgccgcgg agcggtacgc gaccctggcg agccccgtgg tgaccacgaa
     2521 cgctgcggcg ccggtcagca cgagcgtcca gcgcagctcg agggacacca gcgcgagtga
     2581 gagcatgaac agcagcagac aggtcgcgac gacgccacac agggcagcgt tcgcggcgac
     2641 ccccatggtc cggaaacgga tgccggtgcc gcgctggatc agatatccca cgatgatcac
     2701 cccgatcaga accgccatcc ccacgaccag cgggggctgg tagtcgtcgc cgggattggc
     2761 gatcgcaggt gacacgacgc ccaatgccgc gagacctcct agcgccgcca gcagcaccca
     2821 gggcacgcgg atgcgcgccg cgagcctcga tctgtcgtcc gccagcgtgc tcagagcgat
     2881 cgcagcgtcc cttcgagtcg catcggagtt tctttccata tcggcaagtc tatatgagac
     2941 tttccggtac ggcaaacgac atggtgatgt cgccggcatc cgaacccgta ccgcgacccg
     3001 aggtcctggg tcaaggccct gccccaggag cgtgggctca ccgagactga tcgagccgac
     3061 gcatgggagg gcatcgatga caatcaccgc acccgcactc gctggcagcg ctcttcatcc
     3121 acggggcccg ctgggcgagg ccgtgcttga cgcgctgatc gcatccgaac ccgccgatcg
     3181 cacgtcgctg gcctcgcagg cggactcggc cctgcgatgc tgttccgaca tcatgacgga
     3241 tgacgacatc cagttcgcgc tgttcctgct gtacgcctcg gcgtatggaa cgctggacgg
     3301 tctcgatgcg ggccgtgagt gggacccggc gctgatcgcc gtgcgcctgg cgctggagag
     3361 cgcattcgaa caggatctga gggcccgggt cgcgatgccg gatctcccgg aacccaccgc
     3421 cgacgcggtc gcgcgagcgc tgttcgccct cgtcgcggcg gacgacgggc ccagtctgtc
     3481 ccgtcatctg gcgaggcgcg ccaccaacga gcaggcgcac gagttcctgg tgcagcggtc
     3541 gatctacaca ctgcgggagg cggacccgca ctcctgggcg atcccccgcc tgtccggctc
     3601 ccccaaggcc gcactggtcg agatccagtc cgacgagtac ggcggcggac gccccgaccg
     3661 ggtgcatgcc gagctcttcg cgaaggcgct gcgcggcgcc gggctggacg acacctacgg
     3721 cgcatacgtc gacgatgtgc cagcgatcac gctggcctcc cacaacatga tgtcgatgtt
     3781 cgggctgaac cggcggctga tcggcgcgat cgtgggtcac ctcgccgcgt tcgagatgac
     3841 ctcgtcgatc ccgaaccgcc tctacgccga cggactgaga cgactcgggt tcggggacga
     3901 cgtcacggac tacttcgacg agcacgtcga ggcggatgcc gtccacgagc agatcgcggc
     3961 acgcgacctg gccggcggcc tggccgcgca gcatccgcac ctcctgccgg acatcatgtt
     4021 cggcgccgcg gcctgcctgg cgatcgacgg cgctgtcgcc ggtcacatgc tggatgcctg
     4081 ggagcggcag tcgtcctcgt tgcgcacggg ggtgaccgca tgagcgcgtc cgcgccgcag
     4141 ccgacgatca cgccctaccc ggacggaccc ctcctgctgc gcggcgacgt ggtgctgcag
     4201 accgccgacg gcgaggtcat cccccgtcat cgacgcacgg tggcactgtg ccggtgcggg
     4261 ctctcggcca tcaagccgtt ctgcgacggc acgcacaagg cggccggctt ccgcaccgag
     4321 ggctgaggcc gcccgtcagc gggcgcggcg gtagtgcatg tcgacagcac cgctgcgcag
     4381 cggggtcgcg gagatcagct cgagccgccg agtggccggc agcccggcct ggaacagtgt
     4441 cgggccgtgc ccggcgatca tcggctgcac gaggaagcgg tactcgtcga tcaggtccag
     4501 gcggtccagt tcggtggcga gcgatccgct gcccaggagc actccggccg gggtctcgtc
     4561 cttgaggcgc tgcacgccct cgcgcaggtc gccggagagg tgatggctgt tgctccacgg
     4621 gaagtccgtg cgcgtcgacg acacgacgtg cttgggcttg gcatcgagct tgaccgccca
     4681 ctcgcgcatc gcgggcggag cctcctcctc accgcgggcg acggcgggcc agtagccctc
     4741 catcatctcg taggtgacgc ggccccacag catcgccccg gcctcatcca tcaggcacgt
     4801 gaagaaggca tgcgactcgt cgtcggcgat cccctcgcga tgatcgacgc agccgtccag
     4861 cgtgacgttg aggctgaagg tcagggttcc catgacgcga gcctaggtcg ccgcggaggc
     4921 gcccgccagg ggcatcgatg tgggccctgc cgggatcgaa ccgacgacat ccacggtgta
     4981 aacgtggcgc tctaccagct gagctaaagg ccctcgggta tcgatcctag cgggagcccg
     5041 ttgcggcatc cgacccgcga acggatatag gctgagcaca gcgcgtgttg tgccgagccg
     5101 acaaggctcc cccacgtcgc ttcccgtttc cttggcacga cctgccagta agacgaaagg
     5161 ctccacgtga ccgttcacga tcaggatccg tactctcagg gccccctcga cagcgatccg
     5221 gaggagaccg gcgaatggca gcagtcgctc gacgagctgg tcgatgcgaa gggccacggt
     5281 cgcgggcgcg agatcatgct cagcctgctc aagcgctcca aggacctgca cctcggcgtg
     5341 ccgatggtgc cgaccaccga ctacatcaac accatcgccc cggagaacga gccggagttc
     5401 ggcggtgacg aggagatcga gcgccgctat cggcactgga tccgctggaa cgccgcgatc
     5461 acggtgcacc gcgcgcagcg tccgggcatc ggcgtgggcg gtcacatctc cacctacgcc
     5521 ggcgcggcct cgctctacga ggtcggcttc aaccacttct tccgcggcca gaaccacccc
     5581 ggcggcggag accagatctt catccagggc cacgcctccc ccggcatcta cgcccgctcc
     5641 ttcctcgagg ggcgtctgag cgaggccgac atggacggct tccgtcagga gaagtcgcac
     5701 gagggtcatg cgctgccctc ctacccgcac ccgcgctcca tgccggacta ctggcagttc
     5761 ccgaccgtgt cgatgggcct cggcccgatc aacgcgatct accaggcgca gtcgaacaag
     5821 tacctcgcca accgcggcat caaggatacc tccgaccagc acgtctgggc cttcctcggc
     5881 gacggcgaga tggacgaggt cgagtcccgc ggtcagctgc aggtcgccgc gaacgagggc
     5941 ctggacaacc tcactttcgt gatcaactgc aacctgcagc gcctggacgg tccggtccgg
     6001 ggcaacggca agatcatcca ggagctggag tcgttcttcc gcggcgccgg ctggaacgtc
     6061 atcaaggtgg tctggggccg ggagtgggac gacctgctcg cccgcgacac cgacaacgcg
     6121 ctgctgaaca tcatgaacgc caccccggac ggcgactacc agacctacaa ggccgagtcg
     6181 ggcgcgtaca tccgcgagca cttcttcggc aaggacgagc gcgccgcagc cctggtcaag
     6241 gactacaccg acgagcagat ctggcagctg cggcgtggcg gacacgacta ccgcaaggtc
     6301 ttcgccgcct acaaggccgc gctcgagcac aagggccagc ccaccgtcat cctcgccaag
     6361 accgtcaagg gctacggcct gggtccgcac ttcgagggcc gcaacgcgac ccaccagatg
     6421 aagaagatga cgctggagaa cctcaagacc ttccgcgaca ccatgcagat cccgatcacg
     6481 gacgcgcagc tcgaggagaa cccgtacctg ccgccgtact accacccggg cgagaacgac
     6541 gagacgatcc agtacatgct ggagcgccgt cgcgccctgg gcggcttcat cccggagcgc
     6601 cgcacgaagc acaccgcgat cacgctgcct gacgactcca cctacgcgct cccgaagaag
     6661 ggctcgggaa cgcaggagat cgccaccacc atggcgttcg tgcgactgct caaggacctg
     6721 ctgcgctcca aggacttcgg caaccgcatc gtgccgatca tcccggacga ggcgcgcacc
     6781 ttcggcatcg acgcgtactt cccgaccgcg aagatctaca acccgaacgg tcagcactac
     6841 acgtcggtcg accgcgagct gctgctggcc tacaaggaga gcccgcaggg ccagatcgtg
     6901 cacgtcggca tcaacgaggc gggcgccacg gccgcgttca cggctgccgg cacctcgtac
     6961 gccacccagg gcgagccgct catcccgatc tacatcttct actcgatgtt cggataccag
     7021 cgcacgggag acgcgctgtg ggcggccggc gaccagatgg cgcgcggctt cgtcatcggc
     7081 gccaccgcgg gacgcaccac cctcacgggt gagggcctgc agcacgccga cggccactcg
     7141 ccgctgctcg cgtcgaccaa cccggccacc gtgtcctacg accccgcata cggctacgag
     7201 atcgcgcaca tcatgcgctc gggcctggag cggatgtacg gcggcaacca cccgaacccc
     7261 gatgtcatgt actacctcac ggtgtacaac gagccgctcg tgcagccggc cgagcccgag
     7321 gatgtggacg tcgacggcat catccgcggc atccaccgca tctcggtcga ctccggcgag
     7381 ggcctgaagg ctcagctgtt cgcctcgggc gtgggtgtgc cgtgggcgct ggaggcgcag
     7441 gagctgctcc gcaaggactg gaacatcagc gccgacgtgt ggtcggtcac ctcgtggggc
     7501 gagctgcgca acgacggtct cgcggccgac gagcacaact tcctgcaccc cgacgaggag
     7561 ccccgcacgg cctacctcac cgagaagctg cagggcgccg agggtcccgt catcgccgcg
     7621 agcgacttca tgcacgccgt gcaggaccag atccgccagt gggtgccgaa cccgtactac
     7681 acgctcggcg ccgacggctt cggcttctcc gacacccgcg ccgcggcccg tcgcttcttc
     7741 aagatcgacg gtccgtcgat ggtcgtccgc accctgcagg cactggccga ccagggcaag
     7801 atccagcgct ccatcgtcgg cgacgcgatc atcaagtacg cgctgcacga cgtgaacgcc
     7861 ggctccagcg gaaacgcagg cggcgagagc tgaatctgtg acaggtccca ccacgtcggc
     7921 catggacaag gccgccacgc tcgcctggct gcgccggatc tccggtgaca tcgccacggt
     7981 gacgatcaag cggctggagg acaccctccc gtggtacgcc gacatgccac cggcccggcg
     8041 gtccgccgtc gggctggtgg cgcaggcggg catcacctcc ttcatccagt ggtacgagga
     8101 cccgtcctcc accccctgga tcgcggccga catcttcgcc gcggctcccc gcgagctgct
     8161 gcgcagcgtc agtctgcagc agacgctgca gctgatccgc gtgacggtcg cggtgaccga
     8221 ggagcgcgtc gccggccgcg gcgaggatct gcgcgaggcg atcctgctgt actcgcgcga
     8281 cgtcgcgttc gcgtcggcgg acgtgtacgc ccgcgcggcc gaggcccgtg gactgtggga
     8341 tgccaggctc gaggcgctcg tggtggactc catcctcacc ggcgaggccg atgaggagct
     8401 gcccagccgc atcgcggctc tgggctggca cggtcacggc gaggtgtgcg tgctggtcgg
     8461 cacgacgcct ccgcagttcg acgtcgacca ggtccgacgc accgcacggc gcctctcggt
     8521 cgacgtgctg atcggcgtgc aggggtcgcg gctggtgctc gtcgtcggac gcgcccgggt
     8581 tccgggcaag gagacggcgg aggaagagga gctgccgttc gccgagatcg cccagcgcct
     8641 ggagccgtcg ttcggccccg gttacgtggt gctcggtccc cccgtcgccg cactggtgga
     8701 tgcggggcag agcgctcgcg cggcgctggc cggg
//
LOCUS       DATMSX010000578         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_27925,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000578 DATMSX010000000
VERSION     DATMSX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27925"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VN041_18755"
     CDS             complement(<1..513)
                     /locus_tag="VN041_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31127.1"
                     /translation="MSAKTIRLTVADAHSRHDLVVPADSTVAELLTVGGVDLQRYVGT
                     TTSGAAIELDSEVSATPGDGGVIWLFDRTAAAETGRAARSAKEASPAARSRQHWTLLA
                     LLLAIALAMVACTVISPGPVTISVSAAVLLAGALVMLGQPVHGRNTYVAYFAPTLAAA
                     AGALALASFAD"
     gene            600..>1776
                     /locus_tag="VN041_18760"
     CDS             600..>1776
                     /locus_tag="VN041_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccC"
                     /protein_id="HWU31128.1"
                     /translation="MSTTSIRRAPKPPAGGQLTLQQAPELPKPEGASNTLMMALPMVG
                     SLGSVAVLSLTQGGDATRTYIMGGMFLFMALSMVGGQMWRQKSQHSTNVENTRREYLA
                     YLAETRDAVREVATRQRRYEEWMLPAPDTLPFIVEEGSRVWEHTTGSPEFLVTRIGTA
                     TQPLAMTLDEAPIPALAQLDPVSASAAHRFVLTHDQVPDLPFGLDLGTCGRLEIVGDT
                     VRGRGLARAIVAHLATFAAPEDLQIAVIAAGTDLPYWEWAKWLPHAQSDSMTDAVGGA
                     RRIGSSWHEIEPLVVDIRSRGVASGRGGTVLPHLLIVTDGVELPAGHPLAEEGGVDGV
                     TVIDIARDWAELTDPLTIRLIIEGEDGSQLTVARRGYGAVQATPDFLGIASAEAVARR
                     "
BASE COUNT          268 a          646 c          616 g          246 t
ORIGIN      
        1 gtcggcgaac gacgcgagcg cgagcgcgcc ggcggccgcg gccagcgtgg gggcgaagta
       61 ggcgacatag gtgttccggc cgtgcacggg ctgtccgagc atgaccagtg caccggccag
      121 aagcacggcg gcggacacgg agatcgtcac ggggccgggg gagatgacgg tgcaggccac
      181 catcgccagg gcgatggcga ggagcagcgc cagcagcgtc cagtgctggc gcgagcgagc
      241 ggccggcgag gcctccttcg cagatcgcgc cgccctgccg gtctcggctg cagcggtgcg
      301 gtcgaagagc cagatcacgc cgccgtcgcc gggggtggcg gacacctcgc tgtcgagttc
      361 gatggcggca cccgaggtgg tggttccgac gtatcgctgc agatcgacgc cgccgaccgt
      421 cagcagctcg gcgaccgtgg aatcggccgg gaccaccagg tcgtgacggc tgtgcgcgtc
      481 ggcgacggtg aggcgaatcg tcttcgctga catcccccgc ccctcgacgt tcctccgtgc
      541 gacaccggtt catgtcggca tccctgacat gatagtaggc gactcgagga gggccagtcg
      601 tgagcaccac cagcatccgc cgtgcgccga aaccgccggc cggcggccaa ctcactctgc
      661 agcaggctcc ggagctgccc aaaccggaag gcgcgtcgaa caccctgatg atggccctgc
      721 cgatggtggg cagtctcgga tccgtcgcag tcctctccct cactcagggc ggcgacgcca
      781 cgcgcacgta catcatgggc ggcatgttcc tgttcatggc cctgtccatg gtcggcggcc
      841 agatgtggcg gcagaagtcg cagcactcca ccaacgtcga gaacacccgt cgcgagtacc
      901 tcgcctacct cgccgagacc cgggatgccg tgcgcgaggt cgccacccgt cagcggcgct
      961 atgaggagtg gatgctgccg gcccccgaca cgctgccgtt catcgtggag gagggctccc
     1021 gggtctggga gcacaccaca ggcagcccgg agttcctcgt cacccgcatc ggcaccgcca
     1081 cgcagcccct cgccatgacg ctcgacgagg ccccgatccc cgcgctcgcg cagctcgacc
     1141 cggtcagcgc ctccgcagcg catcgtttcg tgctcacgca cgaccaggta cccgacctgc
     1201 ccttcggtct cgacctcggc acgtgcggcc ggctcgagat cgtcggcgac acggtccgcg
     1261 gacgcggcct ggcacgcgcc atcgtcgcgc acctggcgac gttcgcagcc ccggaggacc
     1321 tgcagatcgc ggtcatcgcg gccggcaccg acctgccgta ctgggagtgg gcgaagtggc
     1381 tcccgcacgc ccagtccgac agcatgacgg atgccgtggg cggcgctcgc cggatcggct
     1441 cgtcctggca cgagatcgaa cccctggtcg tggacatccg atcccgcggt gtcgcgagcg
     1501 gacgcggcgg caccgtgctc ccgcatctcc tgatcgtgac cgacggcgtg gagctgccgg
     1561 ccggacatcc gctcgccgag gagggcggcg tcgacggcgt gacggtcatc gacatcgcgc
     1621 gcgactgggc cgagctcacc gacccgctca ccatccgcct gatcatcgag ggtgaggacg
     1681 gctcgcagct cactgtcgcc cgccgtggct acggcgcggt gcaggcgacg cccgacttcc
     1741 tcggcatcgc gtcggccgag gccgtcgccc gccgcc
//
LOCUS       DATMSX010000579         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_75711,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000579 DATMSX010000000
VERSION     DATMSX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75711"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1674
                     /locus_tag="VN041_18765"
     CDS             136..1674
                     /locus_tag="VN041_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWU31129.1"
                     /translation="MTDPTPSPASATSGPSTGTYAHGHKPRSPWPALWALVIGFFMIL
                     VDTTIVSVANPAIKAALDPATNNLDNVVWVTSAYLLAYAVPLLITGRLGDRFGPKNIY
                     LIGLVIFTLSSLACGLSTSLGMLVAFRAVQGLGAAFMTPQTMAVITRTFPPERRGAAM
                     GLWGATAGVATLVGPLLGGLLVDAFGWEWIFYINVPVGIIGFVMAWIFVPKLQTHPHR
                     FDYIGVVLSAAALFLIVFGLQEGQKYDWGVITGPITVWGMIATGIVLLIVFVLQQWKT
                     KSEALVPLGLFKDRNFSASNLGIAAVGFTVTSMSLPFMFFLQLARGLTPTEAALLLIP
                     MAVISGVLAPFAGSLLDRVDPRFILIPGLLCVAGGVLWNSSLMNMDTPTWMFLLPSAL
                     LGIGNAGMWGPLATEATRNLPPRQAGAGAGIYNTTRTIGSVVGSSAIAAFMQARLEAN
                     LPGLDQAPAGLGSGGKMPEAVADGFSAGMSQSMLLPVSVMVVALIAALFIGRYKGKSA
                     DELR"
     gene            complement(1795..2319)
                     /locus_tag="VN041_18770"
     CDS             complement(1795..2319)
                     /locus_tag="VN041_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31130.1"
                     /translation="MSNTLYTPEDVIMQEALTEFTQSLPVWLQWAGVMLAAAIPFVES
                     YFGTLIGIVAGVPLPITLLAAVIGNAASMLAFVFAASAARAKVLARRSGGGAEHAEPS
                     PRRARVKRMFDRFGVPGVSLLGQAVLPSQITSGMMVSFGASRNAVIFWQIISIILWAI
                     IFALIGLGGLALFR"
     gene            2395..2589
                     /locus_tag="VN041_18775"
     CDS             2395..2589
                     /locus_tag="VN041_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU31131.1"
                     /translation="MRSRVKELRGEQGLSQQALATAVGVSRQTINAIETGRYDPSLTL
                     AVHLARHFGSTVEEVFDVDG"
     gene            2576..3016
                     /locus_tag="VN041_18780"
     CDS             2576..3016
                     /locus_tag="VN041_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31132.1"
                     /translation="MSMDEAHEDMKSSRGRKWPMSPVNTVLVGVCVVGAVACFVLGQT
                     IAGTALLLGGIGGGLGAIFARRGTSGDLERVNSLEYADERDRAAAVKGLAAVGVVALI
                     LGAGQLIMHVIVDTDPLSRWTSIGVFLALTVCWFLANWYYVRKG"
     gene            3059..3601
                     /locus_tag="VN041_18785"
     CDS             3059..3601
                     /locus_tag="VN041_18785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWU31133.1"
                     /translation="MTIALACPSLDLFDSWSATVAEFGGVHIDGAGLDRGVVPDRAAC
                     EEFIAKAELYGAADAELPEGHVPCDYFWIIDDGEVVGFIAFRRALNDWLRSYGGHIGY
                     SVRPSRRRQGVARAALDLVVDRARAQGYDRVMLTCDEENVGSYRTIEGAGGVLAGIVD
                     RPADMADDRTGRLRQYWIEL"
     gene            complement(3615..>3777)
                     /locus_tag="VN041_18790"
     CDS             complement(3615..>3777)
                     /locus_tag="VN041_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HWU31134.1"
                     /translation="PGGSLIATGGRVLNVVATGTDFADARARAYEAIGRIRLDGAHYR
                     HDIAARVAQ"
BASE COUNT          559 a         1279 c         1314 g          625 t
ORIGIN      
        1 tcgcctgggt gggcgaccgc ctcacgccgg cggccggcgc cgcggcatcc gatcccgact
       61 cgccgtgacc cctcggcatc cgaacctcca gacactcacc gcgggcagca ccgcacagcg
      121 aaaggcttca cgcccatgac agatcccacg ccgtctccgg catccgcgac cagcgggccg
      181 tcgacgggca cctacgcgca cggccacaag ccgcgcagcc cctggcccgc cctctgggcg
      241 ctggtcatcg gcttcttcat gatcctcgtc gacacgacga tcgtctcggt cgcgaacccg
      301 gccatcaagg ccgcgctcga ccccgccacc aacaacctcg acaacgtggt gtgggtcacc
      361 agcgcctacc tgctggccta cgcggtgccg ctgctgatca ccggacgcct cggcgaccgg
      421 ttcggcccga agaacatcta cctgatcggc ctggtgatct tcacgctctc gtcgctcgca
      481 tgcgggctgt cgacctcgct cgggatgctg gtggccttcc gcgctgtgca gggactcggc
      541 gccgcgttca tgacgccgca gaccatggcc gtgatcaccc gcaccttccc gccggagcgc
      601 cgcggcgccg cgatgggcct gtggggcgcg accgcgggcg tggccacgct ggtcggtcca
      661 ctgctgggcg gcctgctggt ggacgcgttc ggctgggagt ggatcttcta catcaacgtg
      721 cccgtcggca tcatcggctt cgtgatggcc tggatcttcg tgcccaagct gcagacgcat
      781 ccgcaccgct tcgactacat cggcgtcgtg ctcagcgccg cagccctgtt cctgatcgtc
      841 ttcggcctgc aggaggggca gaagtacgac tggggtgtca tcacgggtcc catcaccgtg
      901 tggggcatga tcgctaccgg catcgtgctg ctgatcgtgt tcgtgctgca gcagtggaag
      961 acgaagagcg aggcgctcgt gccgctcggc ctgttcaagg accgcaactt ctcggcatcc
     1021 aacctcggca tcgccgcggt cgggttcacg gtgacgagca tgtcgctgcc gttcatgttc
     1081 ttcctgcagc tggcgcgcgg gctcacgccc accgaggcgg cgctgctgct gatcccgatg
     1141 gcggtgatct cgggcgtgct ggcgccgttc gcgggctcgc tgctcgaccg cgtcgacccg
     1201 cgcttcatcc tgatccccgg gctgctgtgc gtggcgggcg gggtgctgtg gaactcctcc
     1261 ctgatgaaca tggacacccc gacctggatg ttcctgctgc cctccgcgct gctgggcatc
     1321 ggcaacgcgg gcatgtgggg tccgctggcg acagaggcga cgcgcaacct gccgccgcgt
     1381 caggccggcg ccggcgcggg catctacaac accacccgaa cgatcggatc cgtggtcggc
     1441 tcgtcggcca tcgccgcgtt catgcaggcg cggctcgagg cgaacctgcc cggactcgac
     1501 caggcccccg ccgggctcgg ctccggcggc aagatgcccg aggccgtggc tgacgggttc
     1561 tcggccggga tgtcgcagtc gatgctgctg cccgtcagcg tgatggtcgt cgcgctcatc
     1621 gcggccctgt tcatcggccg ctacaagggg aagagcgccg acgaactgcg ctgatcccct
     1681 gcgaacgcat cgagggccgc ccggcttcac gcctgggcgg ccctcgtcgt cttcagacgc
     1741 gactcgtcag aagggcgtca tcgccggaac gggaccatca tcggaacagg gccatcatcg
     1801 gaacagggcc aggccgccca ggccgatcag ggcgaagatg atcgcccaca ggatgatcga
     1861 gatgatctgc cagaagatga ccgcgttgcg gctggctccg aacgacacca tcatgcccga
     1921 ggtgatctgg ctcggcagga cggcctgacc gagaaggctg acgccgggga cgccgaagcg
     1981 gtcgaacatg cgcttgaccc tcgcgcggcg gggggacggc tctgcgtgct cggcgccgcc
     2041 gccgctgcga cgggcgagca ccttcgccct ggcagcggat gccgcgaaca cgaaggcgag
     2101 catggaggcg gcgttgccga tgaccgcggc cagcagggtg atcggcaacg ggacgccggc
     2161 gacgatgccg atcagggtgc cgaaatagga ctcgacgaag gggatcgctg ccgccagcat
     2221 gactccggcc cactgcagcc agacgggcag cgactgggtg aactcggtga gtgcttcctg
     2281 catgatgacg tcctccggtg tgtaaagcgt gtttgacatg acaagcatgc tttacacggc
     2341 gaggcggtgt caagtcagct ttacagatgg ggccgtctgg cctaccgtgg agacgtgcgc
     2401 agcagggtca aagagctcag gggcgagcag ggcctctccc agcaggcgct cgcgaccgcg
     2461 gtgggcgtct cgcggcagac gatcaacgcg atcgagaccg gccgatacga cccctcgctc
     2521 accctcgccg tgcacctggc ccggcacttc ggatcgaccg tggaggaggt cttcgatgtc
     2581 gatggatgag gcgcacgagg acatgaagag ctctcgtgga cggaaatggc cgatgtcgcc
     2641 cgtcaacacg gtgcttgtcg gggtgtgcgt ggtcggcgcc gtggcgtgct tcgtgctcgg
     2701 acagacgatc gccggcaccg cgctgctgct cggcggaatc ggcggtggac tcggcgcgat
     2761 cttcgcacgc cgcggcacct ccggtgatct cgagcgcgtg aactccctgg agtacgccga
     2821 tgagcgcgac cgcgcggccg ccgtgaaggg gcttgccgcg gtgggcgtcg tggcgctgat
     2881 cctcggcgcg ggacagctga tcatgcacgt gatcgtggac accgacccgc tctcccgctg
     2941 gacgagcatc ggcgtgttcc tcgccctcac cgtctgctgg ttcctggcga actggtacta
     3001 cgtgcggaag ggctgaggtc ggtcctcggc ggcgtggatc ctgtgatggg atgggcggat
     3061 gacgatcgcg ctcgcctgtc cgtccctgga cctcttcgac tcctggtctg cgaccgtcgc
     3121 cgagttcggc ggcgtgcaca tcgacggcgc ggggctcgac cgcggcgtgg ttccggatcg
     3181 tgccgcatgc gaggagttca tcgcgaaggc cgagctgtat ggcgcagcgg atgccgagct
     3241 gcccgagggg cacgtgccgt gcgactactt ctggatcatc gatgacggcg aggtggtcgg
     3301 cttcatcgcg ttccggcgtg cgctgaacga ctggctgcga agctacggcg gccacatcgg
     3361 atactcggtg cgaccgtcac gtcgccgaca gggcgtcgcc cgtgcagcgc tggatctcgt
     3421 cgtcgaccgt gcccgtgcgc agggctacga ccgcgtcatg ctgacctgcg acgaggagaa
     3481 cgtcggctcg taccgcacga tcgagggcgc aggcggtgtg ctggccggca tcgtcgaccg
     3541 tcctgccgac atggcggacg accgcaccgg acgcctgcgt cagtactgga tcgagctctg
     3601 acccgacgcc ccggttactg cgcgacgcgt gcggcgatgt cgtggcggta gtgggcgccg
     3661 tccaggcgga tgcgcccgat cgcctcgtac gcgcgcgccc tggcatccgc gaagtcggtg
     3721 ccggtggcga cgacgttcag tacgcgtccg ccagtcgcga tgagcgagcc gccgggt
//
LOCUS       DATMSX010000580        13631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_123160,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000580 DATMSX010000000
VERSION     DATMSX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123160"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1075
                     /locus_tag="VN041_18795"
     CDS             29..1075
                     /locus_tag="VN041_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWU31135.1"
                     /translation="MAAPRSSSRGSGSAAKGTKIPQVPWREPRPAPIVLVSGPEEVLA
                     ERAIAGVRDYLRAEDPALEVSDIRADDYAAGTLLSLTSPSLFGEPRLVRVAGVEKCTD
                     AFLQEAVRYVENPQEGATVILRHTGASVRGKKLLDAVRAGTGGGIEIPCAAIKRDGDR
                     VDFAAGEFKAAKKRIAPTALRSLVSAFADDLTELAAACQQLIGDVEGDITDDVINKYY
                     GGRVEVSAFVVADTAIAGHYGEALIAMRHALASGADPVPMVAAFAMKLRTMARVAGNR
                     EPSRQLAARLGMKDWQVDRARRDLQGWNERSLGMAIQATARADAEVKGAARDPIFALE
                     RMIAVIATREPFGH"
     gene            complement(1163..1423)
                     /gene="rpsT"
                     /locus_tag="VN041_18800"
     CDS             complement(1163..1423)
                     /gene="rpsT"
                     /locus_tag="VN041_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWU31136.1"
                     /translation="MANIKSQIKRNKTNLKAQERNKAVKSELKTLVRKTREAIAGGDK
                     EAAEAALKVASVKLDKAVSKGVVHKNQASNRKSAIAKQVAAL"
     gene            1681..3042
                     /locus_tag="VN041_18805"
     CDS             1681..3042
                     /locus_tag="VN041_18805"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWU31137.1"
                     /translation="MPDSLSAPRIPAGLRWSTATSAFQIEGSRTADGRGRSIWDDFVD
                     RQGAVIDGGTAEPACDSYWRTDDDIELLAGLGVDGYRFSIPWVRIQPGGSGPANSAAL
                     DHYEKLVDGLLARGVDPEPTLYHWELPSEVEAAGGWLSRDTVSRFADYAAMVADRLAD
                     RVATWYTINEAAMTTLQGYGTGELAPAKQLLFGALPTVHHQLLAHAHAAASLRAAGAT
                     RVGITNNHTQVLPAGGSDADLAAAHAYDLIHNRMFSEPLLAGAYPDVEALGLPPMPVQ
                     DGDLELIGDSLDFYGVNFYNPTTVAAASGDSPLPFDLLPTPGAPLTGFGWPIMPEALR
                     DFLVDLSVRHPDMPPIVIGENGASFPEPGDAVHVQDDDRIAFLSAHIDAVAEAVGQGV
                     RVEEYTVWSLMDNFEWADGYTQRFGLVHVDFESGIRTPKASYDWYRDLIRDARSGAEG
                     SAR"
     gene            3039..4274
                     /locus_tag="VN041_18810"
     CDS             3039..4274
                     /locus_tag="VN041_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWU31138.1"
                     /translation="MTGTVKVGAGWMSLFTLAWLAIWTVQLTPVQLLLPLQLDTPEDD
                     WIRGVVSSGLVLGVGGLFGIIAGPLVGAISDRSRSPRGRRRPWALMGVWVSAVFLVLT
                     GLVEGPWLVGACWVGVSIGVSVASAAFTALIADQLPSSQRGAASSAVGSSQAVGIVLG
                     VGLVVLLGLGVLAGYLLLAAIIAVVGTLTALMLPDPPASASEVPPRASDRPRLASLRD
                     RDFAWMLWGRLVANIGNALGTALFLFFLLHGLNQPNAEAQDNLLLLIVVYTVFVVIAS
                     VFTGILSDRTGRRRGLTVAATFVQAASGVAIVILPTFEVTMIAAALMGLGYGAFSTVG
                     LAFATDLLPDEQDHARDLGIVNVTAALGQLIGPVVGAGLVALVGGFWLVFLMAAVLSV
                     AGGLMTALARQPEPGPAPR"
     gene            complement(4225..4947)
                     /locus_tag="VN041_18815"
     CDS             complement(4225..4947)
                     /locus_tag="VN041_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU31139.1"
                     /translation="MTPQVVLVHGIRTSATMWRAQLAHLEAAGVPATAVDLPGHGTRM
                     DEDFTLHEALHTIDAAVREAAAEGPVLLVGHSMGGLLSLAYTGGEDPPPVTALIAASC
                     SALPRGAALTAYRMVAGAVNRLPDRGSWITERILDATIPAETRADFGAGGYALDAQDA
                     ALRSLAALDIATSVARIRIPLWLVNGQLDQLRTHEWLFRRLAPHAELVVVPRTTHLVT
                     AMRPRVFNAVLDLALAVVRERS"
     gene            complement(5828..7540)
                     /locus_tag="VN041_18820"
     CDS             complement(5828..7540)
                     /locus_tag="VN041_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HWU31140.1"
                     /translation="MKQHKLMGALAITGAFALALSGCAANNTGNTGDGGDKGGDTGKK
                     TAFADYNPQPRDNLKQGGTVNFPINEIPEQLNQFNSDGSADTARIAAWYMPQLILFKP
                     DGTPYKNDAYLDEWKNEVKDGNTVITFKFNDKAAWNDGTPMDWTAIDATWKANRSNDE
                     GFVPNSTSGYDNVESVTQGDTPKTAIVTYKGEYPWIAKGFTILLHPAINTPDLFNKAF
                     LSNPHPEWGAGPYTVDQFDANKGFISFKPNDKWWGDKPMLDKVTFTGMDAQASINAFK
                     NGEIDTVGVGTKDRLAQVADMKDIKIYRAQQTANTLVEVDSEKPQFKDVKVREAFFKA
                     INIDQQKKIAWNGLDYSEEPAGSFLMYSFQPGYSDSLAKAGYKYDPKAANKLLDDAGW
                     KKGSDGIREKDGVKLSVVYPVFSDDPTQAALAQSFQKMEKEVGIDLKIDVRPPNKFAA
                     DYNSKNWDVASLRFTSSDPYGVGDMFQLYGADQGLNLSGVWSDETDAEIHEIEKIGNG
                     KEQIAAAMKLEPKLLEGWGLISLYNGPSISAAKKGLANLTPEAYVGLDGFGIQPVENV
                     GWEK"
     gene            complement(7670..9613)
                     /locus_tag="VN041_18825"
     CDS             complement(7670..9613)
                     /locus_tag="VN041_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU31141.1"
                     /translation="MSTPTSKTILSVRDLKVSFASEAGRVDAVRGVSFDLEAGKTLGI
                     VGESGSGKSVTSLAIMGLLDENAKVTGSIQFEGQELLGKSDKQMSMIRGNGMAMVFQD
                     PLTSLTPVFTIGDQLVEALTVHRGLSKSEAWDRSLELLRVVGITNPEKRMKSFPHEFS
                     GGMRQRVVIAIAMANNPRLIICDEPTTALDVTIQAQILDLIEKAQDETGAAVIMITHD
                     MGVVARTADDVMVMYAGKPVEHAPARELFHNTRMPYSIGLLGAIPRVDKAEKEPLIPI
                     KGNPPLLIDLPDACPFASRCPIAIDACVRREPDLVPIASDSGETHLAACIRADQIDSD
                     GLIGGLPVYPSPELHESALTRTPREERGVTLEVRNMVKTFPLLKGALFKRKVGEVHAV
                     KGISFDVREGETMAIVGESGSGKTTTLLQIMDFEKQADGDIVIAGTSVNELHSRKVER
                     TLRRDIQIVFQDPMGALDPRMTVTDIIAEPMQAIGMNKDEIHSRVQELMDLVGLNPAH
                     RDRFPGAFSGGQRQRIGIARALSTNPKIVVLDEPVSALDVSIQAGVINLLDELKVKLG
                     LSYLFVAHDLSVIRHIADRVAVMYLGEFVEYGDVDDVFDNPQHPYTQALLSAIPVPDP
                     DIERTRQRVIFDPETMSTRPATV"
     gene            complement(9610..10542)
                     /locus_tag="VN041_18830"
     CDS             complement(9610..10542)
                     /locus_tag="VN041_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU31142.1"
                     /translation="MSTEMIDPAVQPPVGPDTTAERIPGKQLSKWTLYTRRFMRNKPA
                     VAGIVIFVLMVIYSIVVPLLTPYKIDDQDFMNLSTGPSPEHWFGTNASGNDNWLQTAV
                     GLQRSLMIAVTVSVGVTALSAIVGTAAAYFRGRVEQITLIVIHFLMVVPTFLILALVS
                     NDAGGDWRVISLALIFVSWFFPARVIWTLALSLREREYVDAARYMGVPGMRIVLRHLV
                     PNIGSLLVINFTLGIVGAVSTETGLSFIGFGVKAPDVSLGSLIGYGSASVTSAPWLFY
                     FPALALTLLTVSMALVADGLRDALDPTSAAGGRA"
     gene            complement(10544..11527)
                     /locus_tag="VN041_18835"
     CDS             complement(10544..11527)
                     /locus_tag="VN041_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWU31143.1"
                     /translation="MIKYLVRRALGWLLMIVVATNVTYILAWSFLDPRSNYVGRKPPL
                     SPEQIENLLRPRNLDNTVPLVERWWTWLTGIVLRWDWGVSPTGGSVNEQVAYRMWVSA
                     ELVLGATIIMTLLGIWIGVYTASRQYKLGDRIWQGISIITMNINIIVAALGVVILAIN
                     FNKWVDTRVFFVTGSSSEGVTGFFPVMIDAVQHLILPTIALVVIGYAQYHFLQRTLLL
                     DNISADYVRTARAKGLTKQQAIRRHALRTSLIPVATQVAFSIPGIFTGAILTERIFAW
                     QGMGQYFLTTINGNDVHGVVAVAAFGAVLTAIGALLADIAVVVLDPRVRVS"
     gene            11782..>13631
                     /gene="lepA"
                     /locus_tag="VN041_18840"
     CDS             11782..>13631
                     /gene="lepA"
                     /locus_tag="VN041_18840"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWU31144.1"
                     /translation="MSPRALSPLSPAATPASQIRNFCIIAHIDHGKSTLADRMLQITG
                     VVSDRDMRAQYLDRMDIERERGITIKSQAVRMPWQTDSATGATETFALNMIDTPGHVD
                     FTYEVSRSLAACEGAILLVDAAQGIEAQTLANLYLALENDLTIIPVLNKIDLPAADPD
                     KFAKELADLIGGRPEDVLRVSGKTGAGVEALLDRIVSEIPAPVGDADAPARAMIFDSV
                     YDAYRGVVTYVRMVDGRLEPRERIQMMSTRAAHDLLEIGVSSPEPTPTQGLGVGEVGY
                     LITGVKDVRQSKVGDTVTTLRRPASEALAGYTDPKPMVFSGIYPIDGSDYAELREALD
                     KLKLSDASLQYEPETSVALGFGFRCGFLGLLHLEIITERLSREFGLDLITTAPSVIYE
                     VTTDTGEKVVVTNPSEYPDGRVASVAEPMVKTAILLPKDYVGTVMELCQSRRGALLGM
                     EYFSEERVELRYRMPLGEIVFDFFDQLKSKTQGYASLDYEPDGQQEADLVKVDVLLQG
                     DKVDAFSAIVHREKAYAYGTMMAERLRKLIPRQNFEVPIQAAIGARIIARETIRALRK
                     DVLAKCYGGDISRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSGDVEG"
BASE COUNT         2149 a         4471 c         4620 g         2391 t
ORIGIN      
        1 cgtcggcgcc cgccggtagg ctggagcgat ggcagcacct cgatcgtcct cccgcggcag
       61 cggctccgca gccaagggca cgaagatccc gcaggtgccg tggcgtgagc cccgccccgc
      121 gccgatcgtg ctggtgtccg gacctgagga ggtgctcgcc gagcgggcga tcgccggcgt
      181 gcgcgactat ctgcgggcgg aggacccggc gctcgaggtc agcgacatcc gtgccgacga
      241 ctacgccgcc ggcacgctgc tctcgctcac ctctccctcg ctgttcggcg aaccgcgcct
      301 ggtgcgcgtg gcgggcgtgg agaagtgcac cgacgcgttc ctgcaggagg ccgtgcggta
      361 cgtggagaac ccgcaggagg gcgcgaccgt catcctgcgc cacaccggcg ccagtgtgcg
      421 cggcaagaag ctgctcgacg ccgtccgtgc gggtaccggc ggcgggatcg agatcccgtg
      481 cgcggcgatc aagcgcgacg gtgacagggt cgacttcgcg gcgggcgagt tcaaggcggc
      541 gaagaagcgc atcgcgccca ccgccctgcg ctccctggtc tcggcgttcg ccgatgacct
      601 caccgagctc gcagcagcct gccagcagct gatcggcgac gtcgagggcg acatcaccga
      661 cgacgtcatc aacaagtact acggcggtcg tgtcgaggtg tcggccttcg tcgtcgccga
      721 caccgccatc gccggccact acggggaagc gctgatcgcc atgcgccacg cgctggcatc
      781 cggtgccgat cccgtgccca tggtcgccgc cttcgcgatg aagctgcgca ccatggcgcg
      841 cgtcgccgga aaccgcgagc ccagccggca gctggcagcg cgcctcggca tgaaggactg
      901 gcaggtcgac cgcgcgcgcc gcgacctgca gggctggaac gagcggtcgc tcggcatggc
      961 gatccaggcc actgcccggg cggatgccga ggtcaagggc gccgcccgcg atccgatctt
     1021 cgccctggag cgcatgatcg ctgtcatcgc gacccgtgag ccgttcggac actgagccgc
     1081 ccgcttcctg gctgccgagc ctgtcgacgc aacgcagaag gcccgccccg aaaggggcgg
     1141 gccttctgcg tgaatcggtg gctcagagag cggcgacctg cttggcgatg gccgacttgc
     1201 ggttgctcgc ctggttcttg tgcacgacgc ccttgctgac ggccttgtcg agcttgacgg
     1261 aagcgacctt gagggcggcc tcagcggcct ccttgtcgcc gcccgcgatg gcctcacggg
     1321 tcttgcggac gagggtcttc agctcgctct tgacggcctt gttgcgctcc tgcgccttca
     1381 ggttggtctt gttgcgcttg atctgcgact tgatgtttgc cacgtgtgga cacgctttct
     1441 gtctggatgt tgatcggatg ctgcagacga gagagggcgc cgacagcggt cgtgagggac
     1501 gaacccacac gcaagccaag agtcaatgtt accagcaccc cggcgcagtg cgaaaacggc
     1561 ggctcccgcg cgtcgtgccg cgatcgggtg acgcgccgag tgcgggattc agtcgcgccg
     1621 aacgtggaac cgggtgcaga atcgtcggta cccgatagac gacgacgtca gaggagcccg
     1681 atgcccgatt cgctgtccgc gccccgcatt cctgcgggtc tgcgctggtc caccgccacc
     1741 tccgcattcc agatcgaggg gtcccgcacg gccgacggcc gagggcgctc gatctgggac
     1801 gacttcgtcg accggcaggg tgcggtgatc gacggcggca ccgccgaacc cgcctgcgac
     1861 agctactggc gcacggatga cgacatcgag ctcctcgcgg gcctcggcgt ggacggctac
     1921 cgcttctcga tcccgtgggt gcgcatccag cccggaggat cagggcccgc gaacagcgcg
     1981 gccctcgacc actacgagaa gctcgtggac gggctgctgg cgcgcggcgt cgaccccgag
     2041 cccacgctct accactggga acttcccagc gaggtcgagg cggccggcgg ctggctcagc
     2101 cgcgacaccg tgtcgcggtt cgccgactac gccgcgatgg tcgccgatcg cctggccgac
     2161 cgggtggcga cctggtacac gatcaacgag gccgcgatga ccaccctgca gggctacggc
     2221 acgggggagc tggcgcccgc gaagcagctc ctgttcggcg cgctgcccac cgtgcaccat
     2281 cagctgctcg cgcatgctca cgcggccgcc tccctccgcg cggctggggc gacgcgggtg
     2341 ggcatcacca acaaccacac ccaggtgctc cccgccggag ggtcggacgc cgatctcgcg
     2401 gccgcgcacg cgtacgacct catccacaac cggatgttct ccgagcccct tctggcgggc
     2461 gcgtatcccg acgtcgaggc gctcggcctc ccgccgatgc ccgttcagga cggcgatctc
     2521 gagctgatcg gagattccct ggacttctac ggcgtgaact tctacaaccc caccacggtc
     2581 gccgcagcat ccggcgacag ccctctcccg ttcgatctgc tacccacccc cggcgcgccg
     2641 ctcaccggct tcggctggcc gatcatgccc gaggcgctgc gcgacttcct ggtcgatctc
     2701 tccgtccggc accccgacat gccgccgatc gtcatcggcg agaacggcgc ctcctttccg
     2761 gagcccggtg acgccgtgca cgtgcaggac gacgaccgca tcgccttcct gtcggcgcac
     2821 atcgacgcgg tggccgaggc ggtggggcag ggcgttcgcg tcgaggagta cacggtctgg
     2881 tcgctgatgg acaacttcga gtgggccgac gggtacacgc agcggttcgg gctggtgcat
     2941 gtcgacttcg agtccgggat ccgcacgccc aaggcgtcct acgactggta ccgggatctc
     3001 atccgagacg ctcgctcggg cgctgagggg agcgcacgat gaccggcacg gtgaaggtcg
     3061 gcgccggctg gatgagcctc ttcaccctcg cgtggctcgc catctggacc gtgcagctca
     3121 ctcccgtgca gctgctgctg cccctgcagc tggacacacc cgaagacgac tggatccgcg
     3181 gtgtggtgtc ctccggcctg gtgctcggcg tcggagggct cttcggcatc atcgccggac
     3241 ccctggtcgg cgcgatctcc gaccgttcgc gctctccacg cggtcgtcgc cgtccctggg
     3301 cgctgatggg cgtgtgggtc tcggcggtct tcctcgtgct caccggcctc gtcgaagggc
     3361 cctggctggt cggcgcctgc tgggtgggtg tgtcgatcgg cgtctcggtg gcctccgcgg
     3421 ccttcaccgc gctcatcgcg gatcaactgc cgtcctcgca gcgcggtgcc gcatcctcgg
     3481 cggtgggctc gagccaggcc gtcggcatcg tgctgggcgt gggcctggtg gtgctgctcg
     3541 ggctcggtgt gctggccggc tacctgctgc tggccgcgat catcgcggtg gtcggcaccc
     3601 tgaccgccct gatgctgccc gatccgccgg cctcggcgtc cgaggtgccg ccgcgcgcga
     3661 gcgacagacc acgactcgcg tcactgcgtg accgcgattt cgcctggatg ctctggggca
     3721 ggctcgtggc caacatcgga aacgcgctcg gcacggcgct gttcctgttc ttcctgctgc
     3781 acgggctgaa ccagccgaac gccgaggcgc aggacaacct gctgctgctc atcgtcgtct
     3841 acacggtgtt cgtggtgatc gcctcggtgt tcaccggcat cctctcggat cgcaccggga
     3901 ggcgccgcgg gctgacggtc gccgcgacct tcgtgcaggc ggcgtccggt gtcgccatcg
     3961 tcatcctgcc gaccttcgag gtgacgatga tcgccgcggc gctgatgggt ctgggctacg
     4021 gagcgttctc gaccgtcgga ctcgcctttg cgaccgatct gcttccggat gagcaggacc
     4081 atgcgcggga cctggggatc gtgaacgtca ccgccgcgct cggccagctg atcggccccg
     4141 tggtcggtgc cggcctggtc gcactggtcg ggggattctg gctggtgttc ctgatggcgg
     4201 cggtgctctc tgtggccggc ggcctcatga ccgctctcgc acgacagcca gagccaggtc
     4261 cagcaccgcg ttgaacaccc gcggccgcat ggccgtgacc aggtgcgtag tgcgcggcac
     4321 cacgaccagc tcggcgtgcg gtgcgagccg gcggaacagc cattcgtgcg tgcgcagctg
     4381 gtccagctga ccgttgacca gccacagcgg gatgcggatg cgtgcgaccg acgtggcgat
     4441 gtcgagcgcc gccaggctgc gcagcgcggc gtcctgggca tccagggcgt agccgccggc
     4501 gccgaagtcc gcgcgggtct ccgcgggaat ggtcgcgtcg aggatccgct cggtgatcca
     4561 gctcccgcgg tcggggagcc tgttgacggc tccggcgacc atccggtagg cggtgagggc
     4621 ggcgccgcgg gggagtgcgc tgcacgacgc cgcgatcagc gctgtgaccg gcggcggatc
     4681 ctcgccgccg gtgtatgcca gcgagagcag cccgcccatc gaatgcccga ccaggagcac
     4741 cgggccttcg gccgccgctt cgcggaccgc ggcatcgatc gtgtgcaggg cctcgtgcag
     4801 cgtgaagtcc tcgtccatgc gcgtgccgtg cccgggaagg tcgacggccg tggcggggac
     4861 gcccgccgcc tccagatggg cgagctgcgc acgccacatc gtggcggagg tgcggatgcc
     4921 gtgcaccagg acgacctgcg gagtcacagt cccagcatag aaagaagcgg ggccggtgga
     4981 tgaaccaccg gccccgcttc ctgtgctggg tctgttcagc agatcttctg cccactcggt
     5041 gtgtaacaca tcgtcacggc accgcttccg ctgtagtggt ttccgccggg accagcgtta
     5101 tcgatcttga cgtctgcgct tgctgcggtt gcccccgcga acgtcgtgac gatagcgacg
     5161 actaccgccg cgaacccgaa tccaactcgt cgacttgtct tcatggaaag cttctccttg
     5221 ttgattttcg tgcgttagcg gttgcagttg aggaactgac ccgcttggta gcaatacgcc
     5281 gagtgcagag aaccccctgg cccagcgttg tcgatctttt gatctgcgct cgctgggccg
     5341 gccactgata tcgacgccgc aagcgtgaac actactgccg cgagcccaag aagcgcatgt
     5401 ttcgatctcc ccttcgtacg ctgagccatt ttcttgcctt cctctccggt cgatacgccc
     5461 atgtactgag cgatccgacg ggtcacacat tgaagagcgt tgacccgcca cgccagaata
     5521 tttactggag ttgcgggggc gcaatggttg gaagcgtctt gtcgtgttgc cgtcgcctct
     5581 tgtcgcggaa tccttgcgcg gagcttgccg acgccgaagg ctcgacaccg acggtcaagt
     5641 agtggcggct tgacgccgat tttcgacgaa gcaagtcagg tttcctgcga ggtgagccca
     5701 tcgaccacgt cgtccgtgtg caagcctcgc accggtcgtg tcgcgtcgcg aatacaacag
     5761 gcgaaagcgg ggccggtgga tgaaccaccg gccccgctcc gtgcgatcgg tcgagaaacc
     5821 ctaggggtta cttctcccag ccgacgttct caacgggctg gatgccgaag ccgtcgagac
     5881 ccacgtaggc ctcgggggtc aggttggcca gacccttctt ggctgcggag atcgagggac
     5941 cgttgtagag ggagatgaga ccccagccct cgaggagctt cggctcgagc ttcatcgctg
     6001 ctgcgatctg ctccttgccg ttgccgatct tctcgatctc gtggatttcg gcgtcggtct
     6061 cgtcgctcca gacacccgac aggttcaggc cctggtcggc gccgtagagc tggaacatgt
     6121 cgccgacacc gtacgggtcg gaagaggtga accgcaggct cgcgacatcc cagttcttgc
     6181 tgttgtagtc agccgcgaac ttgttgggcg ggcggacatc gatcttcaga tcgatgccga
     6241 cctccttctc catcttctgg aacgactgcg caagcgcggc ctgcgtcggg tcatcactga
     6301 agacggggta cacgaccgac agcttgacgc cgtccttctc gcggatgccg tcggagccct
     6361 tcttccagcc ggcgtcgtcg aggagcttgt tcgctgcctt cgggtcgtac ttgtaaccgg
     6421 ccttggcgag cgagtcgctg tagcccggct ggaacgagta catcaggaag gaacctgcgg
     6481 gctcctcgga gtagtccagg ccgttccagg cgatcttctt ctgctggtcg atgttgatcg
     6541 ccttgaagaa ggcctcgcgg accttgacgt ccttgaactg cggcttctcg gagtccacct
     6601 cgaccagcgt gttcgcagtc tgctgcgcgc ggtagatctt gatgtccttc atgtccgcga
     6661 cctgggccag gcggtccttc gtgccgacgc cgaccgtgtc gatctcgccg ttcttgaagg
     6721 cgttgatcga ggcctgggcg tccatgccgg tgaaggtcac cttgtcgagc atgggcttgt
     6781 cgccccacca cttgtcgttc ggcttgaacg agatgaagcc cttgttcgcg tcgaactggt
     6841 cgacggtgta cggtccggcg ccccactccg ggtgcgggtt gctcaggaac gccttgttga
     6901 acaggtcagg cgtgttgatc gccgggtgca gcaggatcgt gaatcccttt gcgatccacg
     6961 ggtactcgcc cttgtaggtc acgatggcgg tcttcggcgt gtcaccctgg gtgaccgact
     7021 cgacgttgtc gtagccgctg gtcgagttcg ggacgaagcc ctcgtcgttc gagcggttgg
     7081 ccttccaggt ggcgtcgatg gcggtccagt ccatcggggt gccgtcgttc caggctgcct
     7141 tgtcgttgaa cttgaaggtg atgaccgtgt tgccgtcctt gacttcgttc ttccactcgt
     7201 cgaggtaggc gtcgttcttg tacggcgtgc catccggctt gaacaggatc agctgcggca
     7261 tgtaccacgc tgcgatgcgt gccgtgtccg cgctgccgtc gctgttgaac tggttcagct
     7321 gctccgggat ctcgttgatc gggaagttga cagtgccacc ctgcttgagg ttgtcgcggg
     7381 gctgcgggtt gtagtcagcg aacgccgtct tcttgccggt gtcgccgccc ttgtccccgc
     7441 cgtcaccggt gtttccggtg ttgttggctg cgcacccgct cagagcgagt gcgaaagcgc
     7501 cggtgattgc cagcgctccc atcagcttgt gctgcttcat ggtgctcctt tcgcctttcg
     7561 gcgtgtcatt agggaggtta gttcgcatcg cgtcgcaatc gaggcgtgcg tgagtgaatc
     7621 gtgactaacc gttatcggac ggtgatccga cggttacatc gacgaaatct cagaccgtcg
     7681 cgggccgcgt actcatggtc tccggatcga agatcacgcg ctgacgcgtg cgctcgatgt
     7741 ccgggtcggg aaccgggatg gccgacagca gcgcctgcgt gtacgggtgc tgcgggttgt
     7801 cgaacacatc atccacgtcg ccgtactcga cgaactcgcc gaggtacatg accgcgacgc
     7861 ggtcggcgat atggcggatg accgagagat cgtgcgccac gaagaggtac gacaggccca
     7921 gcttgacctt cagctcgtcg agcaggttga tcacgccggc ctggatcgag acgtccagcg
     7981 ccgagacggg ctcgtcgagc acgacgatct tcgggttagt cgagagagcg cgagcgatcc
     8041 cgatgcgctg ccgctgacca cccgagaacg cgccggggaa gcgatcgcgg tgcgccgggt
     8101 tcaggccgac gaggtccatc agctcctgca cgcggctgtg gatctcgtcc ttgttcatgc
     8161 cgatggcctg catgggctcg gcgatgatgt cggtcacggt catgcgcggg tccagggcgc
     8221 ccatcgggtc ctggaacacg atctggatgt cgcggcgcag ggtgcgctcg actttccggc
     8281 tgtgcagctc gttgacgctg gtgcctgcga tgacgatgtc gccgtcggcc tgcttctcga
     8341 agtccatgat ctgcagcagc gtggtggtct tgccggaacc cgactcgccc acgatcgcca
     8401 tggtctcgcc ctcgcggacg tcgaagctga tgcccttcac tgcgtgcacc tcgcccacct
     8461 tgcgcttgaa cagcgcgccc ttgagcagcg ggaaggtctt gaccatgttg cgcacctcga
     8521 gcgtgacgcc acgctcctcg cgcggagtcc gtgtcagtgc gctctcgtgc aactccggcg
     8581 acgggtatac cggcaagccg ccgatgagac cgtcggagtc gatctgatcc gcgcggatgc
     8641 aggcggcgag gtgggtctcg ccggaatccg acgcgatggg cacgaggtcc ggctcgcggc
     8701 ggacgcacgc gtcgatcgcg atcgggcagc gtgacgcgaa cgggcaggcg tcgggcaaat
     8761 cgatcagcag cggtggattt cccttgatcg ggatcagggg ctccttctcg gccttgtcga
     8821 cgcgcgggat ggctccgagc aggccgatcg agtacggcat ccgggtgttg tggaacagct
     8881 cgcgcgcggg ggcatgctcg acgggcttcc ccgcgtacat caccatcacg tcgtcggccg
     8941 tgcgagccac cacgcccatg tcgtgcgtga tcatgatcac ggcggcgccc gtctcgtcct
     9001 gcgccttctc gatcagatcg aggatctgcg cctggatggt gacgtcgagc gccgtggtgg
     9061 gctcgtcgca gatgatcaga cgcgggttgt tcgccatggc gatcgcgatc accacgcgct
     9121 ggcgcattcc gcccgagaac tcgtgcggga aggacttcat ccgcttctct gggttggtga
     9181 tgccgacgac acgcagcagc tccagcgagc gatcccaggc ctcgctcttg gagagcccgc
     9241 gatgcacggt gagcgcctcg acgagctggt cgccgatcgt gaacaccggg gtgagcgagg
     9301 tcagcgggtc ctggaagacc atggccatgc cgttgccgcg gatcatcgac atctgcttgt
     9361 cggacttgcc aagcagctcc tggccctcga actggatgga tccggtcacc ttggcgttct
     9421 cgtcgagcag gcccatgatc gccagcgacg tgaccgactt gccggatccg gactcgccga
     9481 cgatgccgag ggtcttgccc gcctcgaggt cgaaggatac gccgcgcacg gcatccacac
     9541 gccccgcctc cgaggcgaag ctgaccttga ggtcacggac ggagagaatg gtcttcgaag
     9601 tgggggtgct catgcgcggc ctccagccgc cgaagtggga tcgagggcat cgcggaggcc
     9661 gtcggcgacg agcgccatcg agacggtcag cagcgtcagc gccagggcgg ggaagtagaa
     9721 cagccacggt gcgctggtca cgctggctga gccatatccg atcagggagc cgagggagac
     9781 gtcgggcgcc ttgacgccga agccgatgaa cgacaggccg gtttcggtac tgaccgcacc
     9841 gacgatgccg agggtgaagt tgatcaccag cagcgagccg atgttcggga ccaggtggcg
     9901 cagcacgatg cgcatgccgg ggacacccat gtagcgtgcg gcgtcgacgt actcgcgctc
     9961 ccgcagcgac agcgccagcg tccagatgac gcgggccggg aagaaccagc tcacgaagat
    10021 cagagccagc gagatcacgc gccagtctcc gcccgcgtca ttggagacga gggcgaggat
    10081 gaggaaggtc ggcacgacca tcaggaagtg gatgactatc agcgtgatct gctccacgcg
    10141 gccgcggaag taggcggcgg cggtgcccac gatggccgac aacgcggtga cgcccaccga
    10201 gaccgtgacg gcgatcatca gcgagcgctg caggccgacg gctgtctgca gccagttgtc
    10261 attgccggag gcattggtgc cgaaccagtg ctcgggagag gggccggtcg acaggttcat
    10321 gaagtcctgg tcgtcgatct tgtacggcgt caggagcggg accacgatgg agtagatcac
    10381 catcaggacg aagatcacga tgccggcgac agcaggcttg ttgcgcatga agcgacgcgt
    10441 gtacaacgtc cacttgctca gctgcttgcc cgggatgcgc tcagcggtgg tgtcggggcc
    10501 gacgggcggc tgcacggcgg ggtcgatcat ctcggtgctc atatcagctc accctcactc
    10561 gcgggtcgag gacgacgacg gcgatatcgg ccaggagagc gccgatggcg gtcagcacgg
    10621 cgccgaaggc cgccacggcg accacgccgt gcacgtcgtt tccgttgatg gtggtcagga
    10681 aatactgtcc cattccctgc caggcgaaga tccgctcggt caggatcgcg ccggtgaaga
    10741 tgccggggat ggagaacgcg acctgggtcg ctaccgggat gagcgaggtg cgcagtgcgt
    10801 ggcggcgaat ggcctgctgc ttggtcaggc ccttggcgcg cgcggtgcgc acgtagtcgg
    10861 cgctgatgtt gtccagcagc agggtgcgct gcaggaagtg gtactgcgcg tagccgatga
    10921 ccaccagggc gatcgtgggc aggatgagat gctgcacagc atcgatcatc accgggaaga
    10981 acccggtcac gccctctgag gaggagcccg tgacgaagaa gacccgagtg tccacccact
    11041 tgttgaagtt gatggccagg atcacgacgc cgagtgccgc gacgatgatg ttgatgttca
    11101 tcgtgatgat cgagatgccc tgccagatcc ggtcaccgag cttgtactgg cgggaggccg
    11161 tgtacacgcc gatccagatg cccagcagcg tcatgatgat cgtggcgccg agcacgagct
    11221 ccgcgctgac ccacatgcgg tacgccacct gctcgttgac cgagccgccc gtcgggctga
    11281 cgccccagtc ccagcgcagg acgatcccgg tgagccaggt ccaccaccgc tcgaccaggg
    11341 ggacggtgtt gtccaggttg cggggccgga gcagattctc gatctgctcc ggggagagcg
    11401 ggggctttct gccgacgtaa ttactgcgcg gatcgaggaa ggaccaggcc aggatgtagg
    11461 tgacattggt cgcgaccacg atcatcagca gccagccgag agctcggcgc acgagatact
    11521 tgatcaaggt ggtgattctt tctcttctac agcgcgctcg ggatctgccg acgcaacgct
    11581 gggtcgggat gacattcaag caccatccac gagataacgc acaccggcag cacgggcggc
    11641 tgtgacggag tcctgtgctc cgcggaaccc ggtctccggg tcggggcaac ttcgcgagac
    11701 tatcaccgga cgcgcggaat gcgggattcc ggcgcgctct cgtagacttg cgggaaccct
    11761 tcctgcagca tcaaggacct catgtcaccc cgcgctctct ctccgctgtc gcctgccgcg
    11821 acgcccgcct cgcagatccg caacttctgc atcatcgccc acatcgatca cggcaagtcc
    11881 acccttgccg atcggatgct gcagatcacc ggcgtcgtct ccgaccggga catgcgggcc
    11941 cagtacctgg accgcatgga catcgagcgc gagcgcggca tcaccatcaa gagccaggcc
    12001 gttcggatgc catggcagac cgactccgcc acgggggcga ccgagacgtt cgctctcaac
    12061 atgatcgaca cccccgggca cgtggacttc acctatgagg tgtcccggtc cctcgccgcg
    12121 tgcgagggtg ccatcctgct ggtcgatgcg gcgcagggca tcgaggcgca gacgctggcc
    12181 aacctgtacc tggcgctgga gaacgatctg acgatcatcc ccgtgctcaa caagatcgac
    12241 ctgcccgcgg ccgacccgga caagttcgcc aaggagctcg cggacctcat cggcgggagg
    12301 cccgaggacg tgctgcgcgt ctcgggcaag accggcgcgg gcgtcgaggc gctgctggac
    12361 cgcatcgtca gcgagatccc cgccccggtc ggcgacgccg acgcccccgc gcgcgccatg
    12421 atcttcgact ccgtgtacga cgcctaccgc ggcgtcgtca cctatgtgcg catggtcgac
    12481 ggccgcctcg agccgcgcga gcgcatccag atgatgtcga ccagggccgc gcacgacctt
    12541 ctcgagatcg gcgtctccag ccccgagccg acccccacgc aggggctcgg cgtcggtgag
    12601 gtggggtacc tcatcacggg cgtgaaggac gtgcgccagt ccaaggtggg cgacacggtc
    12661 acgacgctgc gccgaccggc atccgaggcc ctcgccggct acaccgaccc caagccgatg
    12721 gtgttctcgg gcatctaccc gatcgacggc agcgactacg ccgaactgcg agaggcgctg
    12781 gacaagctca agctgtctga tgcgtcgctg cagtacgagc cggagacgtc ggtcgcgctg
    12841 gggttcggct tccgctgcgg cttcctcggt ctgctgcacc tcgagatcat caccgagcgg
    12901 ctctcgcgcg agttcggcct ggacctcatc accacggcgc ccagcgtgat ctacgaggtc
    12961 accaccgaca cgggggagaa ggtagtcgtc accaacccca gcgagtaccc cgacggccgg
    13021 gtcgcctcgg tcgccgagcc gatggtgaag acggccatcc tgctgccgaa ggactacgtc
    13081 ggcacggtca tggagctgtg ccagtcccgg cgcggggcgc tgctgggcat ggagtacttc
    13141 tccgaggagc gcgtcgagct gcgctaccgg atgccgctcg gagagatcgt gttcgacttc
    13201 ttcgaccagc tgaagtccaa gacgcagggc tacgcgagcc tcgactacga gcccgacggc
    13261 cagcaggagg ccgacctcgt gaaggtcgac gtgctgctgc agggtgacaa ggtcgacgcg
    13321 ttcagcgcga tcgtgcacag ggagaaggcg tacgcctacg gcacgatgat ggccgagcgc
    13381 cttcgcaagc tcatcccgcg gcagaacttc gaggtcccga tccaggccgc gatcggtgcc
    13441 cgaatcatcg cccgtgagac catccgcgct ctgcgcaagg acgtgctcgc caagtgctac
    13501 ggcggcgaca tcagccgtaa gcgcaagctg ctcgagaagc agaaggaggg caagaagcgg
    13561 atgaagatgg tcggccgcgt cgaggtgccc caggaggcgt tcatcgccgc gctgtcgggc
    13621 gacgtcgagg g
//
LOCUS       DATMSX010000581         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_179908,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000581 DATMSX010000000
VERSION     DATMSX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179908"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1836
                     /gene="argS"
                     /locus_tag="VN041_18845"
     CDS             169..1836
                     /gene="argS"
                     /locus_tag="VN041_18845"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWU31145.1"
                     /translation="MNPEALAAAILAVLTPIATSLRPDEELGLTASDVVLDRPRNRDH
                     GDWSSNIAMRLAKKLGSNPRELAQRIADDLASVDGISSVEVAGPGFINIRLEAGAAGA
                     LAKTIVDAGAAYGTNDSQAGVSVNVEFVSANPTGPLHIAHTRWAALGDSIVRLLLASG
                     AHAVREYYINDAGAQMERFAQSVLAAAKGEPTPEGGYPGEYITELAQRVLAAHPGLLE
                     LPEDEQLVVARDQAYEYQLAEIKNSLERFNVPFDVWFSERTLHAASEDGGPSLVDQAV
                     DRLRAQGHVFDDEGAVWVRTTDFTDDKDRVIRRSNGEYTYFAADAAYYLNKGDRGFQN
                     KIYLLGADHHGYVNRLKAVAGAAGEDPEKNIQVLIGQMVSINGARLSKRAGNIIEMDD
                     LLNWLGADALRYSLERSPADSPLDLDPELLQKRTNDNPVFYVQYAHARTHNVARNAAD
                     SGVDRSEFAPELLVHETEGALLGVLQEFPRIVAFAAEVREPHRIARYLEELAGLYHRW
                     YDNCRVIPKGDDPIETVHRTRLWLNDAAGQVLRNGLELLGVSAPERM"
     gene            1838..2614
                     /locus_tag="VN041_18850"
     CDS             1838..2614
                     /locus_tag="VN041_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HWU31146.1"
                     /translation="MTAAAPGAEVRRRRARWPWVLLIVVAVIAALLVAAEMVARAVLP
                     GIVRSLVVEELKLPADQQLDVEASGVLLPQLISGTLDELQLSSKQVTIGGITGSAHVT
                     ATGVPTRGGALGSAKGTIAIDQSQFTTLLKNSQLPITGVVLAAPDATVQGELSLLGRE
                     IPVGLTVTPGAQDGDLLLTPVSATLGGAEIDLKRLSGMLGSLGAQLAAPQRICIAEHL
                     PAGITLTGLRIEGTSAVADISADGRIATDPALLENGTCPR"
BASE COUNT          414 a          963 c          913 g          370 t
ORIGIN      
        1 gccgcggccg acgacctgga gatgcgcgcg gtcagcgatg gtgcggtcgg cgcggcgatg
       61 ctcgccatcc gcgccgcggg cggcgtcccc gatgaccgga tcctggcccg tctgacggag
      121 acggtcgccg cctttcgctg agttccgggc cggtagactt gtgggtctat gaatcctgaa
      181 gccctcgccg cagccatcct cgccgtcctg acgccgatcg cgacgtcgct gcgacccgac
      241 gaggagctgg ggctcacggc atccgacgtc gtgctggacc gcccccgcaa tcgcgaccac
      301 ggcgactggt ccagcaacat cgccatgcgg ctggccaaga agctcggatc gaacccgcgg
      361 gagctcgcgc agcgcatcgc cgacgacctg gcgtccgtcg acgggatctc gagcgtcgag
      421 gtcgccggtc ccggattcat caacatccgc ctggaggccg gcgccgccgg agccctcgcg
      481 aagacgatcg tcgacgcggg cgccgcctac ggaaccaacg actcccaggc cggagtctcg
      541 gtgaacgtcg agttcgtctc ggcgaacccg accggcccgc tgcacatcgc acacacccgg
      601 tgggcggcgc tgggcgactc gatcgtgcgc ctgctgctgg ccagcggcgc gcatgccgtg
      661 cgcgagtact acatcaacga cgcgggcgcc cagatggagc gcttcgcgca gtcggtgctg
      721 gcagcagcca agggcgagcc gacgcccgag ggcggctacc cgggcgagta catcaccgag
      781 ctcgcccagc gggtgctcgc cgcgcacccg gggctgctgg agctccccga ggacgagcag
      841 ctcgtcgtcg cccgcgacca ggcctacgag taccagctcg ccgagatcaa gaactcgctc
      901 gagcgcttca acgtgccctt cgacgtgtgg ttctccgagc gcaccctgca cgccgcttcc
      961 gaggacggcg ggcccagcct ggtcgaccag gccgtcgacc ggctgcgggc gcagggacac
     1021 gtcttcgacg acgagggcgc cgtgtgggtg cgcaccactg acttcacgga tgacaaggat
     1081 cgcgtcatcc gccggtccaa cggcgagtac acctacttcg ccgccgatgc cgcctactac
     1141 ctgaacaagg gcgaccgcgg gttccagaac aagatctacc tgctcggggc cgaccaccac
     1201 ggctacgtga accggctcaa ggccgtcgcc ggtgcggcgg gtgaggatcc cgagaagaac
     1261 atccaggtgc tgatcgggca gatggtctcg atcaacgggg cgcgcctgtc caagcgcgcc
     1321 ggcaacatca tcgagatgga cgacctgctg aactggctgg gcgcggacgc gctgcgctac
     1381 tcgctggagc gctcgcccgc cgattcgccc ctggacctgg acccggagct gctgcagaag
     1441 cgcaccaacg acaacccggt cttctacgtg cagtacgcgc acgcgcgcac gcacaacgtc
     1501 gcccgcaacg ccgccgactc cggcgtcgac cgctcggagt tcgccccgga gctgctggtg
     1561 cacgagaccg agggcgcgct gctgggcgtg ctgcaggagt tcccgcgcat cgtcgcgttc
     1621 gcggccgagg tgcgcgagcc gcatcgcatc gcgcgctatc tcgaggagct cgccgggctg
     1681 taccaccgct ggtacgacaa ctgccgggtg atccccaagg gcgacgaccc gatcgagacc
     1741 gtgcaccgca cccgcctctg gctgaacgac gccgcgggcc aggtgctgcg caacggtctg
     1801 gagctgctgg gcgtctcggc gcccgagcgg atgtgacatg acggcagcgg caccaggagc
     1861 ggaggtacgc cgtcggcgtg cgcgctggcc ctgggtgctg ctgatcgtcg tcgcggtgat
     1921 cgcagctctg ctcgtcgccg ccgaaatggt ggcgcgcgcc gtgctgccgg ggatcgtccg
     1981 ctcgctcgtc gtcgaggaac tgaagctgcc ggccgaccag cagctcgacg tggaggcgtc
     2041 cggcgtgctg cttccgcagc tgatctccgg aacgctggac gagctgcagc tgtcatcgaa
     2101 gcaggtcacg atcggcggga tcaccggctc cgcgcacgtg accgccacag gtgtgccgac
     2161 ccgcggcggc gccctgggct cggcgaaggg caccatcgcc atcgatcaga gccagttcac
     2221 gacgctgctg aagaactcgc agctgccgat caccggggtc gtgctggcag caccggatgc
     2281 caccgtgcag ggcgagctca gcctgctcgg acgtgagatc cccgtgggcc tgacggtcac
     2341 ccctggcgct caggacggcg acctgctgct cacgcccgtg tccgcgacgc tcggcggcgc
     2401 tgagatcgac ctgaagaggc tctccgggat gctgggcagt ctcggcgcgc aactcgcggc
     2461 accgcagcgc atctgcatcg ccgagcacct gccggccggc atcacgctca ccggcctgcg
     2521 catcgaggga accagcgcgg tcgccgacat cagcgccgac ggccgcatcg ccacagaccc
     2581 tgccctgctc gagaacggca cctgcccgcg ctgacaggcg tcagggtgct gcgcgctccg
     2641 cacccgcgcg ggccgtccgc
//
LOCUS       DATMSX010000582         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_113463,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000582 DATMSX010000000
VERSION     DATMSX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113463"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN041_18855"
     CDS             <1..521
                     /locus_tag="VN041_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWU31147.1"
                     /translation="RRLAADGLAVQQSPRVTVVADLDADDIRSLFEIRRALEETAARL
                     AAQRGDAAAFTALAAEFAHVDLDGAEGRDAYYALIARLDAQVDASHGNDYLTSALRTV
                     RTHLVRVRRMARDKPERLAASASEHQLIARALASRDAELAAHATHVHLHNALDSILTS
                     LDSPSTLPEGTP"
     gene            518..2047
                     /locus_tag="VN041_18860"
     CDS             518..2047
                     /locus_tag="VN041_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWU31148.1"
                     /translation="MTVTHHVRVYRSEEDLPREEQLAWKIAQVAVDPVEVEPEVVEMI
                     INRIIDNASVAAASLTRGPIVAARAQAFSHPVSTGGKGSTVFGAKLDERTSPEWAAWA
                     NGVAVRELDYHDTFLAAEYSHPGDNIPPILAVAQHAGCDGRALVRGIATGYEIQVDLV
                     RAISLHAHKIDHVAHLGPSAAAGIGTMLGLDTETIYQAIGQALHTTTATRQSRKGEIS
                     TWKAHAPAFAGKMAVEAVDRAMRGQTSPSPIYEGEDGVIAWMLDGKDASYDVPLPAAG
                     EAKRGILDTYTKEHSAEYQAQAIIDLARRLGTARPELRDPANIASIVLHTSHHTHNVI
                     GSGANDPQKYDPKASRETLDHSVPYIFAVALQDGTWHHVDSYLPERAQREDTVALWHK
                     ITTAEDPEWTRRYHSIDPAEKAFGGRVEITLTNGETVIDEIAVADAHPLGARPFGREQ
                     YIRKFRILADPVLDASEIERFLELVQRLPELTAAEVGELSIIAKAGVLASAPEAPKGL
                     F"
     gene            2047..2949
                     /gene="prpB"
                     /locus_tag="VN041_18865"
     CDS             2047..2949
                     /gene="prpB"
                     /locus_tag="VN041_18865"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HWU31149.1"
                     /translation="MLYSSTPAHEKRRLFRERLATGELLRFPGAFNPLSARLIEQKGF
                     EGVYISGAVLSADLGLPDIGLTTLTEVAGRGQQIARMTDLPAIIDADTGFGEPMNVAR
                     TIQTLEDAGLAGAHIEDQINPKRCGHLDGKAVVDEQTALKRIRAAVDARRDENFLIMA
                     RTDIAAVEGLSAAKDRAKALVDAGADAIFPEAMRSLEEFAAIRDAVDVPILANMTEFG
                     KSDLFSVDQLRDVGVNIVIWPVSLLRIAMGAAGRALDTLNEEGHLTSKLGEMQHRADL
                     YDLIDYESYNHFDSGVFNFTVTKG"
     gene            2953..4140
                     /locus_tag="VN041_18870"
     CDS             2953..4140
                     /locus_tag="VN041_18870"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HWU31150.1"
                     /translation="MTEDIKKGLAGVVADVTAISKVNPETNSLLYRGYPVQELAATQP
                     FEAVAYLLWNGELPDADQLAQFRETERAHRALSPRVKDAIDALPLDAHPMDEVRTAVS
                     VIGAIETAGTGNVLDAVGTPEQNLQRSLQLFAVLPAIVAYGQRRRRGQALIESRDDLD
                     YAANFLWLTFGEEPDAVVVDAFNRSMILYAEHSFNASTFTARVITSTLSDLYSAVVGA
                     IGALKGPLHGGANEAVMHIFDEIGTADQVVPWLERALAEKRKIMGFGHRVYKRGDSRV
                     PTMKAALDTLVEHYDRPDVAELYEKLESEFVERKGIYPNLDYPSGPAYNLIGYDTLTF
                     TPLFIAARITGWTAHILEQQASNALIRPLSAYNGVDERHIDSYAPDTAAIEVQERPEE
                     AAG"
     gene            4289..>6343
                     /locus_tag="VN041_18875"
     CDS             4289..>6343
                     /locus_tag="VN041_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWU31151.1"
                     /translation="MTFDPVFDIDRIADPRFVSENRLPAHSDHRWFRDADEARTGVSS
                     FEQSLNGHWAFHCARNQREAPSDFADPGYDVSAWDMIRVPSHLQLEGYDRPQYVNVGY
                     AWDGQEGIVPGQVPERDNPTGCYVRTFTLDTPLQTGERLSVSFHGAESAIAVWLNGRY
                     IGYSTDGFTPGEFDLTDALVEGENRLAAQVFTYSAGSWLEDQDFFRFSGIFRDVVLYR
                     RPVVHAEHLRVRTVVADDLASADIELRVDLAGEGSASARIDGLGELTARGDGALVLHV
                     EDPHLWSPEDPHLHDLVIEVRDAGGAVTEVIPQRVGIRRFGIENGVLSLNGARVVFNG
                     INRHEFSLAGRVPSRELTEADLRVLKAHNVNAVRTSHYPNDSAFYELADEYGLLVIDE
                     MNMETHGRWTEIRQRGAAEDISIPGDHDEWRPAALDRARSMLERDRNHPSVVIWSCGN
                     ESYGGAVLRDVADWFRQADDRPVHYEGVTWDPRYPETTDITSQMYTPAAAVERQLSEH
                     RDKPFILCEYAHAMGNSFGGVAEYVELAYRDELFQGGFIWDFADQAIRMRDRYGREFL
                     GYGGDNGEAPHDHDFSGNGILFADRTLSPKAQEMKKLYQPFVIEVSPAEVAITSRMLF
                     TSSSAFEARVSVAREGRVVASGVLDTDVAPGESGSYPLRVAVPRMPGEYAIVVSLHLR
                     AAT"
BASE COUNT         1044 a         2312 c         2094 g          893 t
ORIGIN      
        1 tgcgccggct cgccgccgac ggtctggccg tgcagcagtc cccgcgggtc acggtcgtcg
       61 cggacctgga tgccgacgac atccgctcgc tcttcgagat acgccgcgcg ctggaggaga
      121 ccgccgcgcg actcgccgcc cagcgcggag atgcggcggc cttcaccgcg ctcgccgcag
      181 agttcgcgca cgtcgacctc gacggggcag agggccggga cgcctactat gcgctgatcg
      241 cccgcctgga cgcgcaggtc gacgcctccc acggcaacga ctacctcacc tccgccctgc
      301 gcaccgtgcg cacccacctc gtgcgcgtgc ggcggatggc gcgcgacaag cccgagcgcc
      361 tggcggcatc ggcatccgag catcagctca tcgcccgcgc cctggcctcc cgcgatgccg
      421 aactcgcggc acacgccacc cacgtgcacc tgcacaacgc cctagacagc atcctgacct
      481 cgctcgacag tccgagcacc ctcccggaag gaaccccatg accgtcaccc atcacgtccg
      541 cgtctaccgc tccgaagagg atctgccccg cgaggagcag ctggcctgga agatcgcaca
      601 ggtcgcggtc gaccctgtcg aggtcgagcc cgaggtcgtc gagatgatca tcaatcgcat
      661 catcgacaac gcgtccgtag ccgcggcatc gctcacccgc ggcccgatcg tcgccgctcg
      721 tgcgcaggcg ttcagccacc cggtatccac cggcggcaag ggctcgaccg tgttcggtgc
      781 gaagctcgac gagcgcacca gccccgagtg ggcagcgtgg gccaacggcg tcgccgtgcg
      841 cgagctcgac taccacgaca cgttcctcgc ggccgagtac tcgcacccgg gcgacaacat
      901 cccgccgatc ctcgcggtcg cccagcacgc cggctgcgac ggacgcgcgc tggtacgcgg
      961 catcgcgact ggctacgaga tccaggtcga cctggtgcgc gccatctcac tgcacgccca
     1021 caagatcgac cacgtcgccc acctgggccc ttcggccgcc gccggcatcg gcaccatgct
     1081 gggcctggat accgagacca tctaccaggc catcggccag gcgctgcaca ccaccaccgc
     1141 cacgcggcag agccgcaagg gcgagatctc cacctggaag gcgcacgcgc cggccttcgc
     1201 cgggaagatg gccgtcgagg ccgtcgaccg cgcgatgcgc ggccagacca gcccgtcgcc
     1261 catctacgag ggcgaggacg gcgtgatcgc ctggatgctg gacggcaagg acgcctccta
     1321 cgacgtgccg ctgcccgccg ccggcgaggc caagcggggc atcctggaca cctacaccaa
     1381 ggagcactcc gccgagtatc aggcgcaggc gatcatcgac ctcgcccgtc gcctgggcac
     1441 ggcccgtccc gagctgcgcg accccgcgaa catcgcgagc atcgtgctgc acaccagcca
     1501 ccacacccac aacgtgatcg gctcgggcgc caacgacccg cagaagtacg accccaaggc
     1561 gtctcgcgag acgctggacc actcggtgcc gtacatcttc gccgtcgccc tgcaggacgg
     1621 cacctggcac cacgtcgact cctacctgcc cgagcgcgcg cagcgcgagg acacggtcgc
     1681 cctctggcac aagatcacca cggccgagga cccggagtgg acccgccgct accactccat
     1741 cgaccccgcc gagaaggcgt tcggcggccg cgtggagatc acgctcacga acggcgagac
     1801 cgtcatcgac gagatcgccg tcgccgacgc ccacccgctg ggcgcccgtc cgttcgggcg
     1861 tgagcagtac atccgcaagt tccgcatcct ggccgacccg gtgctcgacg cctccgagat
     1921 cgagcgcttc ctcgagctgg tgcagcgcct gcccgagctg acagccgccg aggtggggga
     1981 gctgtccatc atcgcgaagg cgggcgtcct ggcatccgcc cctgaggccc cgaaggggtt
     2041 gttctgatgc tgtactccag cactccggcg cacgagaagc gccgtctctt ccgcgagcgt
     2101 ctcgcgaccg gcgagctgct gcgcttccct ggcgcgttca atccgctgtc ggcccggctc
     2161 atcgagcaga agggcttcga gggcgtgtac atctcgggcg ccgtgctctc ggccgatctg
     2221 ggcctccccg acatcgggct gaccaccctc accgaggtgg ccggccgcgg tcagcagatc
     2281 gcccggatga ccgacctccc ggccatcatc gacgccgaca ccgggttcgg tgagccgatg
     2341 aacgtcgccc gcacgatcca gacgctcgag gatgccggcc tcgccggcgc gcacatcgag
     2401 gaccagatca acccgaagcg ctgcggccac ctcgacggca aggccgtggt cgacgagcag
     2461 accgccctca agcgcatccg ggccgccgtc gacgcccgcc gggacgagaa cttcctcatc
     2521 atggcgcgca ccgacatcgc ggcggtggag ggactgtccg ccgcgaagga ccgtgcgaag
     2581 gcgctggtgg atgccggcgc cgacgcgatc ttccccgagg cgatgcgatc gctggaggag
     2641 ttcgccgcga tccgggacgc cgtggacgtg ccgatcctgg cgaacatgac cgagttcggc
     2701 aagagcgacc tgttctcggt cgaccagctg cgcgacgtgg gcgtgaacat cgtcatctgg
     2761 ccggtgtcgc tgctgcggat cgcgatgggc gcggcgggtc gcgcactcga tacgctgaac
     2821 gaggaggggc acctcacctc caagctcggc gagatgcagc accgcgccga tctgtatgac
     2881 ctcatcgact acgagtcgta caaccacttc gactccggcg tcttcaactt caccgtcacg
     2941 aagggctgag caatgaccga ggacatcaag aagggcctcg ccggcgtcgt cgccgacgtc
     3001 accgccatca gcaaggtcaa ccccgagacg aacagcctgc tctaccgcgg gtaccccgtc
     3061 caggagctgg cggcgaccca gccgttcgag gccgtcgcct acctgctgtg gaacggcgag
     3121 ctgccggatg ccgatcagct ggcgcagttc cgcgagaccg agcgggcgca ccgtgcactc
     3181 tcgccacggg tgaaggacgc catcgacgcg ctgccgctgg acgcgcaccc gatggacgag
     3241 gtgcgcaccg ccgtcagcgt gatcggcgcg atcgagacgg caggcaccgg caacgtgctc
     3301 gatgcggtcg gcacgcccga gcagaacctg cagcgcagcc tgcagctgtt cgccgtgctt
     3361 cctgcgatcg tcgcctacgg tcagcggcgc cgccgcggcc aggcgctgat cgaatcgcgc
     3421 gacgacctgg actacgccgc caacttcctc tggctcacct tcggggagga gccggatgcc
     3481 gtggtcgtgg acgccttcaa ccgctcgatg atcctgtacg cggagcactc cttcaacgcc
     3541 tccacgttca cggcccgggt gatcacctcg accctcagcg acctgtactc ggccgtggtc
     3601 ggtgcgatcg gcgcgctgaa gggcccgctg cacggcggcg ccaacgaggc cgtcatgcac
     3661 atcttcgacg agatcggcac ggccgatcag gtggtgccgt ggctggagcg ggcgctcgcc
     3721 gagaagcgca agatcatggg cttcgggcac cgggtgtaca agcgaggcga ctcgcgcgtg
     3781 ccgaccatga aggccgcgct ggacaccctg gtcgagcact acgaccgccc cgatgtcgcc
     3841 gagctgtacg agaagctcga gagcgagttc gtcgagcgga agggcatcta cccgaacctc
     3901 gactacccgt cgggtccggc gtacaacctg atcggctacg acaccctcac gttcaccccg
     3961 ctgttcatcg cggcgcgcat caccggctgg accgcgcaca tcctcgagca gcaggcctcc
     4021 aacgcgctga tccggccgct gtcggcgtac aacggcgtcg acgagcgcca catcgacagc
     4081 tacgcgcccg acaccgccgc gatcgaggtg caggagcgtc ccgaggaggc ggcgggctga
     4141 ccggcatccg cggctccgcc tcccggtgag gcatcgggca cgaggttcag cgttacggcg
     4201 cggacctcgt gcccgatctc gtccgggggt gacgccgagg aatgttcacg ttatcattct
     4261 ggtgtcatcc gagcagggga gccaccacgt gacattcgat cccgtcttcg acatcgaccg
     4321 catcgccgac ccgcgcttcg tctcggagaa ccgcctgccg gcgcactccg accaccgctg
     4381 gttccgggac gccgacgagg cgcgcaccgg cgtcagctcc ttcgagcagt ccctcaacgg
     4441 acactgggcg ttccactgcg cacgcaatca gcgcgaggcg ccgtcggact tcgccgaccc
     4501 gggatacgac gtctccgcct gggacatgat ccgggtgccc tcgcacctgc agctggaggg
     4561 gtacgaccgc ccccagtacg tcaacgtcgg gtacgcgtgg gatgggcagg agggcatcgt
     4621 ccccggtcag gtgcccgagc gcgacaaccc gaccggatgc tatgtccgca cgttcacgct
     4681 ggacaccccg ctgcagacag gcgaacgcct gagcgtgagc ttccacggcg cggagagcgc
     4741 gatcgcggtg tggctgaacg ggcggtacat cgggtacagc accgacggct tcacgcccgg
     4801 ggagttcgac ctgacggacg cactcgtcga gggcgagaac cgtctggccg ctcaggtctt
     4861 cacctacagc gccggatcgt ggctcgagga ccaggacttc ttccgcttct ccggcatctt
     4921 ccgcgacgtc gtgctgtacc gccgtcccgt cgtgcacgcc gagcacctgc gcgtgcggac
     4981 cgtggtcgcc gacgacctgg catccgccga catcgagctg cgtgtggatc tcgcaggcga
     5041 aggttcggca tccgcgcgca tcgacgggct cggcgagctg accgcccgcg gcgacggcgc
     5101 cctggtgctg catgtcgagg atccgcacct gtggagcccg gaggacccgc acctgcacga
     5161 cctggtcatc gaggtgcggg acgcgggcgg ggctgtgacc gaggtgatcc cgcagcgcgt
     5221 cggcatccgc cgcttcggca tcgagaacgg cgtgctctcg ctcaacggcg cgcgcgtcgt
     5281 gttcaacggc atcaaccgtc acgagttctc cctggccgga cgcgtgccgt cgagggagct
     5341 gaccgaggcc gacctgcggg tgctgaaggc ccacaacgtc aacgccgtgc gcaccagcca
     5401 ctaccccaac gactccgcgt tctacgaact ggccgacgag tacgggctgc tcgtgatcga
     5461 cgagatgaac atggagacgc acgggcgctg gaccgagatc cgccagcgcg gcgcggcgga
     5521 ggacatctcc atccccggcg accacgacga atggcggccc gcggcgctgg accgcgcgcg
     5581 cagcatgctc gagcgcgacc gcaaccaccc gagcgtcgtg atctggtcgt gcggcaacga
     5641 gtcgtacggc ggcgccgtgc tgcgggacgt ggccgattgg ttccgccagg cggatgaccg
     5701 gcccgtccac tacgagggtg tgacctggga cccgcggtat cccgagacga ctgacatcac
     5761 gagccagatg tacaccccgg ccgccgccgt cgagcggcag ctgagcgagc accgggacaa
     5821 gccgttcatc ctgtgcgagt acgcgcacgc gatgggcaac tccttcggcg gtgtggcgga
     5881 gtacgtcgag ctcgcgtacc gcgacgagct gttccagggc ggattcatct gggacttcgc
     5941 ggatcaggcc atccggatgc gcgaccggta cgggcgcgag ttcctgggct acggcggcga
     6001 caacggcgag gcgccgcacg accacgactt cagcggcaac gggatcctct tcgcggaccg
     6061 caccctctcg cccaaggcgc aggagatgaa gaagctgtac cagcccttcg tcatcgaggt
     6121 ctcgccggcc gaggtcgcga tcacgagcag gatgctgttc acctcgtcgt cggccttcga
     6181 ggcgcgcgtg tcggtcgctc gcgagggcag ggtcgtggcc tccggcgtcc tcgacaccga
     6241 cgtcgctccg ggcgagagcg gctcgtaccc gctgcgggtg gcggtgccgc ggatgccggg
     6301 ggagtacgcg atcgtcgtgt cgctgcacct gcgcgccgcg acg
//
LOCUS       DATMSX010000583         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_84660,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000583 DATMSX010000000
VERSION     DATMSX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84660"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1503
                     /locus_tag="VN041_18880"
     CDS             <1..>1503
                     /locus_tag="VN041_18880"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU31152.1"
                     /translation="SAAEMLALVGIPRPQERMGDFPHQLSGGMRQRVMIAMALICEPR
                     VLIADEPTTALDVTIQAEILELLGELKQQLGMAMILVTHDMGVVVRHADRVGVMYGGR
                     LVETGRTADLFRSTRHQYTHALLASIPTLAQDKDVELFSIPGSPPDPADPQIGCRFAP
                     RCAAATERCRTETPRPVAGPGEGHLFACWNPVDERQATREVRRFDASGIASSETDAQP
                     VRLSVVDLRKEHPVSAKGVFGRRRSVKAVAGVSFEVRAGEAFGLVGESGCGKSSLGRM
                     LVALDAPTSGEVLLDGDRVSGLSPRRLGSRRAKLQMMFQDSHASLDPKMRIDAILCEP
                     MIIQGIGTAEERAERARALLAGVGLGASILERYPHELSGGQRQRIGLARALALDPDVL
                     VADEPVSALDVSIRSQVLNLMRGIQRERGLSSVVISHDLAVVRYLVDRVGVMYLGALV
                     EVGATDEVYTRPAHPYTAGLLAAVPVADPDLADTPVDRVHGELPSPLDPP"
BASE COUNT          203 a          536 c          547 g          217 t
ORIGIN      
        1 gcagcgccgc cgagatgctc gcgctggtcg gcatcccgcg cccgcaggag cgcatgggcg
       61 acttcccgca ccagctgtcc ggcggcatgc gccagcgggt gatgatcgcg atggcgctga
      121 tctgcgagcc gcgcgtgctc atcgccgacg agccgaccac cgcgctggat gtgaccatcc
      181 aggccgagat cctggagctg ctgggcgagc tgaagcagca gctcgggatg gccatgatcc
      241 tcgtcacgca tgacatgggc gtggtggtcc ggcatgccga ccgcgtcggc gtcatgtacg
      301 gcggacgcct ggtcgagacc ggccgcacgg ccgacctgtt ccgcagcacg cggcatcagt
      361 acacgcacgc gctgctggcc tcgatcccga cgctcgctca ggacaaggac gtcgagctgt
      421 tcagcatccc cggatcgccg ccggacccgg ccgatccgca gatcggatgc cgtttcgccc
      481 cgcgctgcgc ggccgcgacc gagcggtgcc ggaccgagac tcctcggccg gtggccggcc
      541 ccggagaagg gcacctgttc gcgtgctgga acccggtgga cgagaggcag gcgacgcgcg
      601 aggtccgtcg cttcgacgca tcggggatcg cgtcatccga gaccgacgcg cagccggtgc
      661 gcctgagcgt cgtcgacctg cgcaaggagc atcccgtctc ggcgaagggc gtcttcgggc
      721 gccgccgctc cgtcaaggcc gtcgcgggcg tgagcttcga ggtacgcgca ggtgaggcgt
      781 tcgggctcgt cggcgagtcg ggctgcggca agtcctcgct cgggcggatg ctggtcgcgc
      841 tcgacgcacc tacctcgggc gaggtgctgc tggacggcga ccgggtgtcg gggctcagcc
      901 cgcgacgcct gggctcacga cgcgcgaagc tgcagatgat gttccaggac tcgcacgcgt
      961 cgctggatcc caagatgcgc atcgacgcga tcctgtgcga gccgatgatc atccagggca
     1021 tcggcaccgc ggaggagcgc gccgagcggg ccagagccct gctcgccggc gtcggcctcg
     1081 gggcctcgat cctggagcgc tatccgcacg agctgtccgg cgggcagcgt cagcgcatcg
     1141 gcctggcccg cgcgctggcc ctggaccccg atgtgctggt cgccgatgag ccggtcagcg
     1201 ccctcgacgt gtcgatccgc tcgcaggtgc tgaacctgat gcgggggatc cagcgggagc
     1261 gcggcctgtc cagcgtggtg atctcgcacg acctcgccgt ggtccgctac ctcgtcgacc
     1321 gggtcggcgt gatgtacctg ggcgcgctgg tcgaggtcgg cgcgacggac gaggtgtaca
     1381 cgcggccggc gcacccgtac accgccggtc tgctggccgc cgtgcccgtc gccgaccccg
     1441 atctggccga cacccccgtc gaccgcgtgc acggcgagct ccccagtccg ctggacccgc
     1501 cga
//
LOCUS       DATMSX010000584         8732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_179961,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000584 DATMSX010000000
VERSION     DATMSX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179961"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VN041_18885"
     CDS             complement(<1..204)
                     /locus_tag="VN041_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31153.1"
                     /translation="MADENDDKNTPTLDTTPAQPVVEEAPVAETDESVIAEPDAAPAA
                     DAEADAGSAADVSSTEATSDGESE"
     gene            474..1082
                     /locus_tag="VN041_18890"
     CDS             474..1082
                     /locus_tag="VN041_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HWU31154.1"
                     /translation="MTAQRTRHIGYGIWLILASVVGWWAAFQLTMDKFISLENPQAEL
                     GCNLSFFVQCGANLDSWQGSVFGFPNPIIGLTGWMAVLVMGVAVVAGVRFPRWFSALF
                     GVGITAAFAFIAWLIWQTFFALHTLCPWCGLTYLVMIPTFLTTVVHLGRNGTLSKKKS
                     VQEAFGRLMAWTPLASILCFAVIVAMAYMAGGDLIGELLRLL"
     gene            complement(1140..1556)
                     /gene="ndk"
                     /locus_tag="VN041_18895"
     CDS             complement(1140..1556)
                     /gene="ndk"
                     /locus_tag="VN041_18895"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWU31155.1"
                     /translation="MATEETLVLVKPDGVARGLTGTILARIEAKGYALVDIRLVEPDR
                     DLLAAHYAEHEGKPFYEPLLEFMMSGPSVAIRLAGNRVIEGFRSLAGTTDPTTAAPGT
                     IRGDFGRDWGLKVQQNLVHGSDSPESAARELGIWFS"
     gene            complement(1558..1968)
                     /locus_tag="VN041_18900"
     CDS             complement(1558..1968)
                     /locus_tag="VN041_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HWU31156.1"
                     /translation="MTASRAPRARTPRTLAQKLGAIVLGFEAIVVFLAGLTVYGLNVL
                     PQGIPPWWGIVGGIVLAVAMIALSGAIARPGAIAIGWGLQVLVLASALLVPAIALVAL
                     IFGGMWGYATIVGARMDRRQADTHAQPETHTESE"
     gene            complement(1965..3317)
                     /locus_tag="VN041_18905"
     CDS             complement(1965..3317)
                     /locus_tag="VN041_18905"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWU31157.1"
                     /translation="MSDVQRADAVYEKLLERAGERWVQPRKERVARILTLLDDPQRTY
                     RVVHITGTNGKTSTARMIESLLRAHGLRTGLFTSPHLKRFTERIMIDGEPIADAAVAD
                     AWDEIEPFVGIVDAELEAAGDAPLTFFELLTVLAFVAAADAPVDVLVLEVGMGGSWDS
                     TNTADGDVAVFAPIDMDHADRLGDTIEKIAEVKAGIIKDGAAVVSAEQNPEAEAVLRR
                     VAAEKNATIAFEGEDFGVSAQKLAVGGQMVSIRGLAGEYADEYLPLYGAHQGHNAALA
                     VVAVESLIGGGSQRIAGEIITDGLQNATSPGRLQLVGIAPTVVVDAAHNPHGAASLAR
                     ALDDSFDFDEWGLVLGVFADKDTAGIVDTLLPAVTEVFATSPDSERAEEADAIADLVE
                     ERGRRASVHPTLAEAVDAAREWASASERRAVVIAGSIVLAGEALLLSEEEDWKSGWRG
                     "
     gene            complement(3314..6640)
                     /gene="ileS"
                     /locus_tag="VN041_18910"
     CDS             complement(3314..6640)
                     /gene="ileS"
                     /locus_tag="VN041_18910"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWU31158.1"
                     /translation="MTYPRTSGTAFGPAAVTPSPRFPEIETEVLDFWKTDDTFRASIA
                     QREGADEWVFYDGPPFANGLPHYGHLLTGYAKDLFPRFQTMLGKKVDRVFGWDTHGLP
                     AELEAMKQLGLTEKSEIEDMGIGVFNGKARDSVLAYTREWQDYVTRQARWVDFERGYK
                     TLDLGYMESVLWAFKTLFDKGLAYEGYRVLPYCWRDETPLSNHELRMDDDVYQDRQDP
                     SVTVTFPLTGAKAESLGLTAVRALAWTTTPWTLPTNLALVVGPEIEYVVLPAGPNGAA
                     DVVTGDDPAETAAHRYLLARDLLGGYAKDLGYESAEEALAAVDRSVLGKELEDVTYDR
                     LFDYYADAETWGTEKAWRILVDDYVTTTDGTGIVHQAPAYGEDDQRVSNAAGIPTILS
                     LDDGGKFLPNVTDVAGVLWMEANTPLIRLLRAAGRMLREQSYVHSYPHCWRCRNPLIY
                     KAVSSWFIRVTDIKDRMLANNEQITWVPENVKHGQFGKWLEGARDWSISRNRYWGSPI
                     PIWKSDDPTYPRVDAYGSLEELERDFGTLPHNENGEVDLHRPYIDDLTRPNPDDPTGK
                     STMRRIEDVFDVWFDSGSMPYAQVHYPFENQEWFDSHAPADFIVEYIGQTRGWFYVMH
                     VLSTALFDRPAFTGVSCHGIVLGSDGYKMSKSLRNYPDVSEVLDRDGSDAMRWFLMSS
                     SVLRGGNLAVTEEGIRAGVREFLLPLWNSWYFFATYANAATPEGYEATWRTDSTDVLD
                     RYILARLGDLVRDVRADLEGLDSTTASARLRDFAEVLTNWYIRRSRDRFWEGTATEAF
                     DTLYTVLETLTRVAAPLVPLISERIWQGLTGGRSVHLTDWPDAEAFPAADDVRDAMDA
                     VRELSSVGNALRKKEKLRVRLPLARLTVVSPLDDDLAQFEGILREELNVKTVELVKLG
                     EDTAREYGISHRLSVNARAAGPRLGKDVQKVIRAAKEGFWTEDAGVVTADGIALEPHE
                     YELVLETTGRPEGEALAVLPTGGFVLLDTATTPELEAEGLARDVIRAVQDTRKKAGFD
                     VSDRIRLQLRFTDAADADAVESAFALADVAGETLAVEHSLLRADAELGESEFENVIGK
                     GTYANRGDFTVAVARTGAAQ"
     gene            complement(6863..7549)
                     /locus_tag="VN041_18915"
     CDS             complement(6863..7549)
                     /locus_tag="VN041_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31159.1"
                     /translation="MTTTVQQIVYSRHPQRWHALAEALGFVAPHPPEADWAEFDGGGV
                     LAVHREIDDHPDATVDLHLLVDDLDAAQQALAGRDVTRTTLEGVGNMLLVRLASGLGI
                     SISAGARATTGPMAVQPILFQEDLAEARGVLEDLGLRADIVADRGGWVELHASGGGSV
                     GVHEASEPAVGLGFLADGDLDALAARLRDAGFEASVVDEAYARTVRIAEPEAWINGVQ
                     TDLYGYHREA"
     gene            complement(7546..7698)
                     /locus_tag="VN041_18920"
     CDS             complement(7546..7698)
                     /locus_tag="VN041_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HWU31160.1"
                     /translation="MQSSDAKVQFNVYLPPGVVRAVKHRAIDEESSLSALVERALRQY
                     LEGVES"
     gene            7766..8416
                     /locus_tag="VN041_18925"
     CDS             7766..8416
                     /locus_tag="VN041_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWU31161.1"
                     /translation="MARRSEDLRPPLVSAPDLPDHLDPAPPRRSADLIAALIELEGDA
                     DLAHSSLEQCVLTGGADSLDLRGATLMDVELRDLRVADLPARTASLRRVRISGGRIGT
                     LDLTDTRIHELELHGVRIDYLTLSATKAVDVRIDGCRITALDLPGATLTRVAFDGTHA
                     DEVDSRGLQASHVDLRGLDALSFLDVASLRGTTLTVRQVELLAPAFAASAGISVRD"
     gene            complement(8442..>8732)
                     /locus_tag="VN041_18930"
     CDS             complement(8442..>8732)
                     /locus_tag="VN041_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWU31162.1"
                     /translation="AIARALAADPQVLVLDEPVSALDPTVRQRVLDLLGRLQRERALT
                     MVFVSHDLAVIAAVSDDVLVMQDGVIVEQGAVADVFADPRHPFTRELLAAGR"
BASE COUNT         1240 a         3047 c         3026 g         1419 t
ORIGIN      
        1 ctcggactcg ccgtccgacg tcgcctccgt cgacgagacg tccgccgcgg aaccggcgtc
       61 cgcctcggca tccgccgcag gcgcagcatc cggctccgcg atgacggact cgtccgtctc
      121 cgcgaccggg gcctcctcga ctaccggctg cgccggcgtc gtgtcgaggg taggggtgtt
      181 cttgtcatcg ttctcatcgg ccatctctgg ctagctccct gcgcgggcac tgcgtgcgcc
      241 gcgaaaactc gtgcggcgaa acagcagccg cgaactcaat cggcatgcga tccgctcagt
      301 ggctggatcg cgtgggacat ccggtcccga agtcttgtcg atgcggtcgt gacttctcac
      361 gacgacacct gcgtccatta tcgcatcccg atcgttcgac ggtgtcatcc gagtgtgcgg
      421 cgcgcgtcat gtcctcaccc gaaccgcata gcgaacgctg ggataatccc gatatgaccg
      481 cacagcgcac acggcacatc gggtacggca tctggctcat cctcgcgagc gtggtgggct
      541 ggtgggcggc cttccagctc acgatggaca agttcatcag cctggagaac ccccaggcgg
      601 agctgggctg caacctgagc ttcttcgtgc agtgcggcgc gaatctcgac tcctggcagg
      661 gctcggtgtt cggcttcccg aacccgatca tcggcctcac cggctggatg gccgtgctcg
      721 tcatgggcgt cgccgtcgtc gcgggagtgc ggttcccgcg ctggttctcg gccctcttcg
      781 gagtcggcat caccgcagcg ttcgcgttca tcgcgtggct gatctggcag acgttcttcg
      841 ccctccacac gctgtgcccc tggtgtggtc tgacctacct cgtgatgatt cccaccttcc
      901 tcaccacggt ggtgcacctc ggccggaacg ggacgctgtc gaagaagaag tccgtgcagg
      961 aggccttcgg ccgtctgatg gcctggactc cgctcgcctc gatcctgtgc ttcgccgtca
     1021 tcgtcgcgat ggcctacatg gcgggtgggg atctgatcgg cgagcttctg cgcctcctct
     1081 gatccgaacg cgaaagaggg acgccctgtc ggggcgtccc tctttcatgt tctcggggtt
     1141 cagctgaacc agatgccgag ctcgcgcgcc gccgactccg ggctgtccga gccgtgcacg
     1201 aggttctgct gcaccttcag tccccagtcg cggccgaagt cgccgcggat cgtgccgggc
     1261 gcggcggtgg tcgggtcggt ggtgcctgcg agggagcgga agccctcgat gacgcggttg
     1321 ccggccaggc ggatcgccac ggacggaccc gacatcatga attcgaggag cggctcgtag
     1381 aacggcttgc cctcgtgctc ggcgtagtgc gcggccagca ggtcgcggtc gggctcgacc
     1441 aggcggatgt cgacgagcgc gtagcccttc gcctcgatgc gggcgaggat cgtgccggtc
     1501 aggccgcggg cgacgccgtc gggcttgacg aggacgaggg tttcttcagt ggccatgtca
     1561 ttcgctctct gtgtgagtct cgggctgtgc gtgggtgtcc gcctgcctgc ggtccatccg
     1621 ggcccccacg atcgtcgcat atccccacat gccgccgaaa atcagcgcga cgagcgcgat
     1681 ggcggggacg agcagggcgg atgccaggac gagcacctgc agcccccacc cgatcgcgat
     1741 cgcaccgggg cgggcgatcg caccggacag ggcgatcatc gccacggcga ggacgatgcc
     1801 gccgacgatt ccccaccatg gggggatccc ctgcggcagt acgttcagcc cgtacaccgt
     1861 caggccggcg aggaagacga cgatcgcctc gaagcccagc acgatcgcgc cgagcttctg
     1921 cgcaagagtg cgtggtgtcc tggcgcgggg cgcgcgcgag gccgtcatcc gcgccatccg
     1981 ctcttccagt cctcttcctc ggagagcagc agcgcctcgc ctgcgagcac gatcgacccg
     2041 gcgatcacga ccgcacgccg ctcggaggcc gacgcccact cgcgcgcggc gtccacggcc
     2101 tcggcgagcg tggggtgcac gctcgcgcgg cgtccgcgct cctcgaccag gtcggcgatg
     2161 gcatccgcct cctcggcgcg ctcggagtcg ggcgaggtcg cgaacacctc ggtgacggcg
     2221 gggagcaggg tgtcgacgat gcctgcggtg tccttgtcgg cgaagacgcc cagcaccagc
     2281 ccccactcgt cgaagtcgaa gctgtcgtcc agggcgcgcg ccagcgacgc cgcaccatgc
     2341 gggttgtgcg cagcgtccac gaccacggtc ggcgcgatgc cgaccagctg gaggcggccc
     2401 ggagaggtgg cgttctgcag cccgtcggtg atgatctcac cggcgatgcg ctgtgagccg
     2461 ccgccgatca gcgattccac ggcgacgacg gcgagcgccg cgttgtggcc ctggtgcgcg
     2521 ccgtacagcg gcaggtactc gtcggcgtac tcgccggcga gcccgcggat gctgaccatc
     2581 tgaccgccca cggcgagctt ctgcgccgac acgccgaagt cctccccctc gaacgcgatg
     2641 gtcgcattct tctcggcggc gacgcggcgc agcaccgcct cggcctcggg gttctgctcc
     2701 gcggagacga cggccgcgcc gtccttgatg atccctgcct tgacctcggc gatcttctcg
     2761 atcgtgtcgc ccagccggtc ggcgtggtcc atgtcgatcg gggcgaacac cgccacgtct
     2821 ccgtcggcgg tgttcgtgga gtcccacgaa ccgcccatgc cgacctcgag cacgagcacg
     2881 tcgaccgggg catccgccgc cgccacgaag gcgagcaccg tgagcagctc gaagaaggtc
     2941 agcggggcat cgccggcggc ctcgagctcg gcgtcgacga tgccgacgaa cggctcgatc
     3001 tcatcccacg cgtcggcgac cgccgcatcc gcgatcggct cgccgtcgat catgatgcgc
     3061 tccgtgaagc gcttcaggtg cgggctggtg aagaggccgg tgcgcagccc gtgggcgcgc
     3121 agcaggctct cgatcatccg cgcggtcgac gtcttcccgt tggtgccggt gatgtgcacg
     3181 acgcggtagg tgcgctgcgg atcgtcgagc agggtgagga tgcgggcgac ccgctccttg
     3241 cggggctgca cccaccgctc ccctgcacgc tccagcagct tctcgtagac ggcgtccgcc
     3301 ctctgcacgt cgctcactgc gctgctcccg tccgggcgac ggcgaccgtg aagtcgccgc
     3361 ggttggcgta ggtgcccttg ccgatcacgt tctcgaactc gctctcgccg agctcggcgt
     3421 ccgcacgcag caggctgtgc tcgaccgcga gggtctcgcc tgcgacatcc gccagggcga
     3481 acgccgactc cacggcatcc gcatccgcag catccgtgaa gcgcagctgc aggcggatcc
     3541 ggtcgctcac gtcgaagccg gccttcttgc gggtgtcctg cacggcgcgg atcacgtcgc
     3601 gggccagacc ctcggcctcg agctccggtg tggtcgcggt gtcgagcagc acgaacccgc
     3661 ccgtgggcag caccgcgagc gcctctccct cgggtcgccc ggtggtctcc agcaccagct
     3721 cgtactcgtg cggctccagc gcgatgccgt cggctgtcac gacgccggcg tcctcggtcc
     3781 agaagccctc cttcgcggcg cggatgacct tctgcacgtc cttgcccagt cgggggcccg
     3841 cggcacgggc gttgacgctg agacgatggc tgatgccgta ctcccgggct gtgtcctcgc
     3901 cgagcttcac cagctccacc gtcttgacgt tgagctcctc gcggaggatg ccctcgaact
     3961 gcgcgaggtc gtcgtcaagc ggcgagacga cagtgaggcg ggcgagcggc agccgcacgc
     4021 gcagcttctc cttcttgcgc agggcgttgc cgacgctgga cagctcgcgc acggcgtcca
     4081 tggcatcccg cacgtcatcc gccgcaggga acgcctcggc gtccggccag tccgtcaggt
     4141 gcacgctgcg cccgccggtc agtccctgcc agatgcgctc gctgatcagc ggcaccagcg
     4201 gcgccgcgac gcgggtgagg gtctcgagca ccgtgtagag cgtatcgaac gcctcggtgg
     4261 cggtcccctc ccagaagcgg tcgcgcgagc ggcggatgta ccagttcgtc agcacctcgg
     4321 cgaagtcgcg caggcgagcg gacgccgtgg tcgagtccag gccctccaga tcagcgcgca
     4381 cgtcgcggac gaggtcgccg agccgggcga ggatgtaccg gtccaggacg tccgtggaat
     4441 cggtgcgcca ggtcgcctcg taaccctccg gggtcgcggc gttcgcgtag gtggcgaaga
     4501 agtaccacga gttccacagc ggcagcagga actcccgcac gcccgcgcgg atcccctcct
     4561 ccgtgacggc gaggttcccg ccgcgcagca ccgagctgga catcaggaac cagcgcatgg
     4621 cgtccgaacc gtcccggtcc agcacctcgc tgacgtcggg gtagttgcgc agcgacttcg
     4681 acatcttgta gccgtcggag ccgagcacga tgccgtggca gctgaccccg gtgaacgccg
     4741 ggcggtcgaa cagggcggtg gagagcacgt gcatcacgta gaaccagccg cgggtctgcc
     4801 cgatgtactc cacgatgaag tcggccggag cgtgcgagtc gaaccactcc tggttctcga
     4861 acgggtagtg cacctgcgcg tacggcatcg agccggagtc gaaccacacg tcgaagacgt
     4921 cctcgatgcg gcgcatcgtg gacttcccgg tcgggtcgtc ggggttcggc cgggtgaggt
     4981 cgtcgatgta gggccggtgc aggtcgacct caccgttctc gttgtgcggg agcgtgccga
     5041 agtcgcgctc cagctcctcc agcgagccgt aggcgtccac tcgggggtag gtcgggtcgt
     5101 cgctcttcca gatcgggatc ggcgagcccc agtagcggtt gcggctgatc gaccagtcgc
     5161 gcgcaccctc gagccacttg ccgaactggc cgtgcttgac gttctccggc acccaggtga
     5221 tctgctcgtt gttcgcgagc atccgatcct tgatgtcggt cacgcggatg aaccagctcg
     5281 agaccgcctt gtagatcagg gggttccggc agcgccagca gtgcgggtag gagtgcacgt
     5341 agctctgctc gcgcagcatc cgccccgccg cgcgcagcag gcggatcagg ggcgtgttgg
     5401 cctccatcca gagcacgccg gcgacgtcgg tgacgttcgg caggaacttg ccgccgtcgt
     5461 cgagcgagag gatggtcggg atgcccgcgg cattggagac gcgctggtcg tcctcaccgt
     5521 aggcgggtgc ctggtgcacg atgccggtgc cgtcggtggt ggtgacgtag tcgtcgacga
     5581 ggatgcgcca cgccttctcc gtgccccagg tctctgcgtc cgcgtagtag tcgaacagcc
     5641 ggtcgtaggt gacgtcctcg agctccttgc cgagcacgga gcggtcgact gcggcgagcg
     5701 cctcctcggc cgactcgtag ccgaggtcct tcgcgtagcc gcccagcagg tcgcgggcca
     5761 gcaggtagcg atgcgcggcg gtctcggccg ggtcgtcccc cgtcacgacg tcggctgcac
     5821 cgttcggccc ggccggcagc accacgtact cgatctcggg acccacgacg agggccaggt
     5881 tcgtcgggag cgtccacggg gtcgtggtcc aggcgagggc gcgcacggcg gtgaggccga
     5941 gggattcggc cttggccccg gtgagcggga acgtgacggt gacggagggg tcctgccggt
     6001 cctggtagac gtcgtcgtcc atgcgcagct cgtggttgga cagcggcgtc tcatcgcgcc
     6061 agcagtacgg cagcacgcgg tagccctcgt aggcgagccc cttgtcgaac agggtcttga
     6121 acgcccacag cacgctctcc atgtacccga ggtcgagcgt cttgtagccg cgctcgaagt
     6181 cgacccagcg ggcctgacgg gtcacgtagt cctgccattc gcgcgtgtac gcgagcacgg
     6241 agtcgcgcgc ctttccgttg aagacgccga tgcccatgtc ctcgatctcg ctcttctcgg
     6301 tgagtccgag ctgcttcatc gcctcgagtt cggcgggcag gccgtgcgtg tcccatccga
     6361 acacgcggtc gaccttcttg ccgagcatgg tctggaagcg cgggaacagg tccttggcgt
     6421 acccggtgag caggtgaccg tagtgcggca ggccgttcgc gaagggaggg ccgtcgtaga
     6481 agacccactc atcggcgccc tcgcgctgcg cgatggaggc gcggaaggtg tcatccgtct
     6541 tccagaagtc gaggacctcg gtctcgatct cggggaagcg cgggctcgga gtgacggcgg
     6601 ccgggccgaa ggccgtgcct gaggtgcgcg gataggtcat cgtgtctcgc agtggtcgtg
     6661 ggcggatgct gctgccggga cgacccgtgc tgcgacggac cgcggtacca cccgacgtgc
     6721 ttctcgcgaa gccactctca ctgcggcgat gacgggcctg ccccgctcgg ttctagtggc
     6781 cccgccgagg cgggacgttc ttccgagagc tccccggtga tggccggatc agtgcttgtc
     6841 cggccattct acgcgcatcc ggtcaggctt cgcggtggta gccgtacagg tcggtctgga
     6901 cgccgttgat ccaggcctcc ggttccgcga tgcggacggt gcgggcgtac gcctcgtcga
     6961 cgacggacgc ctcgaacccg gcatccctca accgcgcggc gagcgcgtcg aggtcaccgt
     7021 cggcgaggaa gcccaagccg acagcgggct cggatgcctc gtggactccg acggacccgc
     7081 cgccggacgc gtgcagctcc acccagccac cgcggtcggc gacgatgtca gcgcgaagtc
     7141 cgaggtcttc gaggacgcct cgggcctcag cgaggtcctc ctggaacagg atcggctgca
     7201 ccgccatcgg gccggttgtc gcgcgggcgc ctgccgagat gctgatgccg agaccggacg
     7261 cgaggcgcac gagaagcatg ttcccgacgc cctccagtgt cgtacgcgtg acgtcgcgcc
     7321 cggcgagcgc ctgctgcgcg gcgtcgaggt cgtcgaccag caggtgcaga tccacggtgg
     7381 cgtccgggtg atcatcgatc tcgcggtgca cggcgagcac gcctcccccg tcgaattcgg
     7441 cccagtccgc ctccggcggg tggggcgcga cgaatcccag ggcctcggcg agggcgtgcc
     7501 agcgctgcgg atgccgggag tagacgatct gctgcacggt cgtggtcacg actcgacccc
     7561 ttccaggtac tggcgcagtg cgcgttcgac gagggccgag aggctggact cctcgtcgat
     7621 cgcgcggtgc ttgaccgcgc ggaccacgcc gggcggcaga tagacgttga actgcacctt
     7681 cgcgtcgctg gactgcatgc tagcaatata gcgacgatca cgagggcgcg gcaagccgtc
     7741 ggtgccctcg cctagagtcg agcgcatggc ccgccgcagt gaggatctcc gcccgcccct
     7801 cgtgtccgca cccgatctgc ccgatcacct ggaccccgcg ccgccccgcc ggagcgcgga
     7861 tctgatcgcc gcgctgatcg agctggaggg cgacgccgac ctggcgcact cctccctgga
     7921 gcagtgcgtg ctgaccggcg gcgcggattc cctcgacctt cgcggcgcca ccctcatgga
     7981 tgtcgagctg cgggacctgc gcgtcgcgga tctcccggcg cgcacggcga gcctgcgccg
     8041 ggtccgcatc tccggcggac ggatcggcac cctcgatctg accgacaccc gcatccacga
     8101 gctcgaactg cacggcgtcc ggatcgacta cctgacgctg tccgccacga aggccgtgga
     8161 cgtgcgcatc gacggatgcc ggatcaccgc cctcgatctg cccggcgcca cgctcacccg
     8221 cgtcgcgttc gacggcacgc acgcggacga ggtcgactcc cgcgggctgc aggcctcgca
     8281 cgtggatctc cgcggtctgg acgcgctctc gttcctcgac gtcgcatcgc tgcgcggcac
     8341 gaccctcacc gtccggcagg tggagctgct ggcgcccgcg ttcgcggcat ccgccgggat
     8401 ctcggtgcgg gactgacgcg acccgatcgc gtgccgcggg atcagcgccc tgccgcgagc
     8461 agctcccggg tgaacggatg ccgggggtcg gcgaacacgt cggccaccgc gccctgctcc
     8521 acgatcaccc cgtcctgcat gaccagcacg tcgtcgctga ccgcggcgat gaccgcgagg
     8581 tcgtgcgaga cgaacaccat cgtgagcgcg cgctcccgct gcaggcggcc gagcaggtcg
     8641 agcacacgct gtcgcaccgt cgggtccagg gccgagacag gctcgtcgag gaccagcacc
     8701 tgcgggtcgg cggcgagcgc acgggcgatc gc
//
LOCUS       DATMSX010000585         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_169996,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000585 DATMSX010000000
VERSION     DATMSX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169996"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VN041_18935"
     CDS             <1..642
                     /locus_tag="VN041_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWU31163.1"
                     /translation="AAKAAVAAAEISAATGVEAHSVAFDITDAAAVDAGMTEVESVIG
                     TPDILVNNAGIQRRAPIAEFAEEDWDALVGTNLSGVFHLARRTSQGMIERGSGKIIQI
                     GSVQSQLARPSIAAYSATKGAIVMFTKGLCADLAPHGIQANAIAPGYFETALTQALVD
                     DEQFSAWVRGRTPAGRWGDVRDLVGALVFLASPASDFVNGQTLFVDGGMTAVV"
     gene            642..1373
                     /locus_tag="VN041_18940"
     CDS             642..1373
                     /locus_tag="VN041_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWU31164.1"
                     /translation="MLIGRVRTDDDIRWVRPDGARMQGTEDPFAALAAGRSPGDTEQE
                     VHGDVLPPCEPVVVVGIAQNGSGHASPVQAWLKSPRTVIASGRPVRLRRDAGQAVAEG
                     EIAVVIGRDTTGLTVENAHEYVLGVTAVNDLSSPDRGEFDPRNFESKSGEGYTPLGPW
                     IDTDADIDDIPLTVAVDGVVIASTGAVELPVTIRECLAYVASWSPLGPGDVVMTGAPY
                     SQAPIRPGQTVTITVGAVELRTPFS"
     gene            1404..>1829
                     /locus_tag="VN041_18945"
     CDS             1404..>1829
                     /locus_tag="VN041_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWU31165.1"
                     /translation="MSNIAVVAHAADDLRVEEIEEPHPAPDEAIVEIAYGGICGSDLH
                     YWMHGAAGASVLREPMILGHEVSGVVSAAAADGTGPAVGARVAVHPLTPHDDGRTPWP
                     HEHPNLAPASTYLGSAMLLPHTQGAFARRAALPARMLHEI"
BASE COUNT          270 a          679 c          626 g          254 t
ORIGIN      
        1 gccgcgaagg ccgcggtggc cgccgcggag atctcggcgg ccaccggggt ggaggcgcac
       61 agcgtggcgt tcgacatcac cgacgccgcc gccgtggacg ccgggatgac cgaggtcgaa
      121 tccgtgatcg gcacccccga catcctcgtc aacaacgccg gcatccagcg acgtgcaccg
      181 atcgccgagt tcgccgagga ggactgggat gccctcgtcg gcaccaacct ctccggcgtc
      241 ttccacctcg cccgccgcac ctcccaaggc atgatcgagc gcggctccgg caagatcatc
      301 cagatcggca gcgtgcagtc gcagctggcg cgtccgtcga tcgccgcgta ctccgcgacg
      361 aagggcgcga tcgtgatgtt cacgaaggga ctgtgtgcgg atctcgcgcc gcacggcatc
      421 caggccaacg ccatcgcgcc cggctacttc gagaccgccc tgacgcaggc gctcgtggat
      481 gacgagcagt tctccgcatg ggtcaggggc cgcactccgg ccggccgctg gggcgacgtg
      541 cgcgacctcg tcggcgccct cgtgttcctg gcgagccctg ccagcgactt cgtcaacggg
      601 cagacgctgt tcgtcgacgg cggcatgacg gcggtcgtgt gatgctcatc ggacgggtgc
      661 gcaccgacga cgacatccgc tgggtgcgtc cggacggcgc gcggatgcag ggcacggagg
      721 atccgttcgc ggcgctcgcc gcggggcgct cgcctggcga cacggagcag gaggtgcacg
      781 gcgatgtgct tccgccgtgc gagccggtcg tcgtggtcgg gatcgcgcag aacggatcgg
      841 ggcatgcctc tccggtgcag gcctggctga agagcccgcg caccgtcatc gcatccggcc
      901 ggcccgtgcg ccttcgaagg gacgccggac aggccgtcgc ggagggagag atcgccgtcg
      961 tcatcggccg cgacaccacc ggcctcaccg tcgagaacgc gcacgagtac gtgctgggcg
     1021 tcaccgccgt gaacgacctc tccagccctg accgcggcga gttcgacccg cgcaacttcg
     1081 agtcgaagtc gggggagggc tacacgcccc tcggcccgtg gatcgacacg gatgccgaca
     1141 tcgacgacat cccgctcacc gtcgcggtcg acggcgtcgt catcgcctcc accggtgcgg
     1201 tggagctgcc ggtcaccatc cgcgagtgcc tcgcctacgt cgcttcctgg tctccgctcg
     1261 ggcccggcga cgtcgtgatg accggcgccc cgtattccca ggcgccgatc aggcccgggc
     1321 agaccgtgac catcaccgtc ggtgcggtgg agctccgcac cccgttcagc tgacagccgc
     1381 cgcaaccgcg aaaggagcat gcgatgtcga acatcgccgt cgtcgcacac gccgcagacg
     1441 atctgcgcgt cgaggagatc gaagagccgc accctgcccc cgacgaggcg atcgtggaga
     1501 tcgcctacgg cggcatctgc ggctccgacc tgcactactg gatgcacggc gcggcggggg
     1561 cgtccgtgct gcgggagccg atgatcctcg gacacgaggt gtccggcgtc gtctccgccg
     1621 ccgcggccga cgggacgggc cccgccgtgg gcgcccgcgt cgccgtgcat ccgctcaccc
     1681 cgcacgacga cggacgcacc ccttggccgc acgagcatcc gaacctcgcg cccgcatcca
     1741 cctacctcgg ctcggcgatg ctgctgccgc acacccaggg cgccttcgcg cgtcgcgccg
     1801 cactgccggc ccgcatgctg cacgagatc
//
LOCUS       DATMSX010000586         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_151127,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000586 DATMSX010000000
VERSION     DATMSX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151127"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..>1593)
                     /locus_tag="VN041_18950"
     CDS             complement(11..>1593)
                     /locus_tag="VN041_18950"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HWU31166.1"
                     /translation="ADAVTGAQVKVDATLTAGAASGTTFAQLRVAGAVEATIRPLDEI
                     QEFRSWTEGLGMKQLDVLVPELFAVGQGRTRTLDVIVENFSAQSRSGSVSLTLPAGST
                     ASPTQQPYSGLAAGQTTTVSFEVTNTDTSIPTANRAPNKGSWPVKVTASSEAGTAERA
                     VTMNLVPTYAVQRAASAPVIDAVRGDGEYPGEAIPIDTIWDGSPMGGTNPSISAKTWI
                     THDDANFYLFVSVVDDVRGTILPASDNKRQRRTDSVEIYFDPRGTAGNTAQTFIAGIM
                     PSMDSMTGAPGVGRDRDNWQGEAAITAPGMQVAVKMAPTAAEYTGYDMEVKIPFAVLP
                     DNLDPQHVGFNVVINDSDTQNKAAQNRVGWSTFPGMRADPWRWGIITLDGVQDAGSKP
                     KSPTLPDTAARSVSSPQSILQSAADQVPLGGFSPADHALKVLKSSISGGFVTLKLQTP
                     RAGTARVFLWDGAQVLGSAEVKLAAGASDVRFSARTAATGGGSSLTGTKASPVLAVSF
                     ETSSGVYAARAPLGGRSA"
BASE COUNT          211 a          539 c          585 g          258 t
ORIGIN      
        1 cgtccgttcg tcaggccgat cggccgccca gcggcgcccg ggcggcgtac acgccgctgc
       61 tcgtctcgaa cgagacagcg aggaccggcg aggccttggt gccggtgagg ctgctgccgc
      121 cgcccgtggc cgccgtgcgc gcggagaacc gcacatccga ggcgcccgcc gccagtttca
      181 cctccgcgct gcccagcacc tgagcgccgt cccacaggaa cacccgcgca gtgccggccc
      241 gcggcgtctg cagcttgagc gtgacgaagc ctccggagat cgatgacttc agcaccttca
      301 gagcgtggtc ggcaggtgag aacccgccga gcgggacctg atcggccgcg gactgcagga
      361 tcgactgcgg cgacgacacg gaccgcgccg cggtgtcggg gagggtcgga ctcttcggct
      421 tcgagccggc gtcctggacg ccgtcgaggg tgatgatgcc ccagcgccac gggtcggcgc
      481 gcatgccggg gaacgtcgac cagcccacgc ggttctgcgc cgccttgttc tgcgtgtcgg
      541 agtcgttgat cacgacgttg aagccgacgt gctgcgggtc gaggttgtcg ggcagcactg
      601 cgaacgggat cttcacctcc atgtcgtagc ccgtgtactc agccgcggtc ggcgccatct
      661 tcaccgcgac ctgcatcccg ggtgcggtga tcgcagcctc accctgccag ttgtcacggt
      721 cgcggccgac gccgggcgca ccggtcatcg agtccatcga tggcatgatc cccgcgatga
      781 aggtctgggc ggtgttgccc gcggtgccgc gggggtcgaa gtagatctcc accgagtccg
      841 tgcggcgctg gcgcttgttg tccgaggcgg gcaggatcgt gccgcgcacg tcgtcgacga
      901 ccgagacgaa caggtagaag ttcgcgtcgt cgtgggtgat ccacgtcttc gcgctgatcg
      961 acgggttcgt cccgcccatc gggctgccgt cccagatcgt gtcgatgggg atcgcctcgc
     1021 cggggtactc gccgtcgccg cggacggcgt cgatcaccgg tgcggatgct gcgcgctgca
     1081 cggcgtacgt cggaacgagg ttcatcgtga cggcgcgctc ggcggtgccc gcctcgctgg
     1141 acgcggtgac cttcacgggc cagctgccct tgttgggtgc gcggttggcg gtggggatcg
     1201 aggtgtcggt gttggtcacc tcgaagctga cggtggtggt ctgcccggcg gcgagccccg
     1261 agtacggctg ctgggtgggc gacgcggtgg acccggcagg cagcgtcagc gagacggaac
     1321 cggagcgact ctgcgccgag aagttctcga cgatcacgtc gagcgtgcgg gtgcgtccct
     1381 ggccgacggc gaacagctcg gggacgagca cgtcgagctg cttcatgccc aggccctcgg
     1441 tccaggaccg gaactcctgg atctcgtcga gcgggcggat cgtcgcctcc acggcgccgg
     1501 cgacgcgcag ctgcgcgaac gtcgttccgc tggccgcgcc cgcggtgagc gtggcgtcca
     1561 ccttcacctg ggcgccggtg acggcatccg ctc
//
LOCUS       DATMSX010000587         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_180016,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000587 DATMSX010000000
VERSION     DATMSX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180016"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..153)
                     /locus_tag="VN041_18955"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="VN041_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
     gene            146..325
                     /locus_tag="VN041_18960"
     CDS             146..325
                     /locus_tag="VN041_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31167.1"
                     /translation="MQWFLVGVIGGFVAAHFMNKDPRGHDILAEVDARINGFTALIGN
                     AYREQEARLTEPGDN"
     gene            381..>2878
                     /gene="alaS"
                     /locus_tag="VN041_18965"
     CDS             381..>2878
                     /gene="alaS"
                     /locus_tag="VN041_18965"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWU31168.1"
                     /translation="MKTAEIAQRYLDFFEKNDHLIVPSASLVSDDPSLLFTVAGMVPM
                     IPYLTGVVPAPHPRIADLQKCIRTNDIEEVGRTARHGTFFQMLGNWSFGDYFKEGAIR
                     YAWELLTSSEADGGLGFDEKDLWVTVYETDDEAEAIWRDEIGLKPERIQRLGRADNYW
                     NTGQPGPGGPDSEIFFDRGPSYGKDGGPAADDTRFLEIWNLVFMQDFIENIRGKTEFD
                     IVGELPMKNIDTGMGLERVAFLKQGVENMYETDQVRPVLDRAVELSGKKYGSVHEDDV
                     RFRVIADHVRSSLMLLSDGVRPSNEGRGYILRRLMRRTVRAMRLLGVDEPVFPELFAT
                     SRDAMKSAYPVLETEWATLSSAAFAEEDTFRRTLAQGSTILDLALEDTKKSGGSTLSG
                     SEAFLLHDTYGFPIDLTLEVAEEAGLDVDRSAFDSLMKEQRERAKADARNRKRQLADV
                     SVYRDFRAAGETVFAGYTDLQTDSRILGIIVDGAPVRAASEGQVAEVILAETTLYAES
                     GGQVSDKGTIVGTGFELDVLDVQKPVPGLISHTVHVASGSVAVDDRATTVVDAPNRRA
                     ARQAHSATHLVHAALRDTLGKTATQAGSLNRAGYMRFDFAWSQPLSAETRSEIEDITN
                     RAVQDALEVTTRIVSLDEAKEAGAMALFGEKYGDVVRMVDIGGPWSRELCAGTHVSSS
                     SEIGLVSVVGESSVGASNRRIEALVGQDAFRELAAERTVVSQLSAALKAPRDQLSTRI
                     EELQANLKAAEKRIAQFEAKEREGRVPALADAAQQVGSFRVASTSLGEIGSADDVRTL
                     ALSVRERLGTDAAVVAFGGVANGRPIVVVATNDAA"
BASE COUNT          487 a         1008 c          941 g          442 t
ORIGIN      
        1 ctcgtagacg gtgacccaga ggtccttctc gtcgaagccc aggccgccgt cggattcgga
       61 gctcgtcagc agctcccagg cgtagaagat cgcgccttcc ttgaagtagt agccgaacga
      121 ccaattgcac agcatctgga agaacgtgca atggttcctg gtcggtgtga tcggcggctt
      181 cgtcgcggct cacttcatga acaaggatcc gcgcggtcac gacatcctcg ccgaggtcga
      241 cgccaggatc aacggattca ccgcgctcat cggcaacgcc taccgcgagc aggaggcacg
      301 gctcacggag ccgggcgaca actgaaccac cgcagcagcg ccacggcgcg gcatcttcac
      361 gcaaggacac ccggcactct atgaaaactg cggagatcgc gcagcgttac ctcgatttct
      421 tcgagaagaa cgaccacctc atcgtcccct cggcctccct ggtcagcgac gacccgtcgt
      481 tgctgttcac ggtcgccggc atggtcccga tgatcccgta cctcacgggc gtcgtccccg
      541 cgccgcatcc tcgcatcgcc gacctgcaga agtgcatccg caccaacgac atcgaagagg
      601 tcggccgcac ggcgcggcat ggcacgttct tccagatgct cggcaactgg tcgttcggcg
      661 actacttcaa agagggcgcg atccgctacg cgtgggagct gctgacctcg tccgaggcgg
      721 acggcggcct cggcttcgat gagaaggacc tctgggtcac cgtctacgag accgacgacg
      781 aggccgaggc gatctggcgc gacgagatcg gcctgaagcc cgagcgcatc cagcgcctgg
      841 gacgggcaga caactactgg aacaccggtc agcccggccc tggtggcccg gactcggaga
      901 tcttcttcga ccgcggcccg tcgtacggca aggacggtgg acccgccgcc gatgacacac
      961 ggttcctgga gatctggaac ctcgtgttca tgcaggactt catcgagaac atccgcggca
     1021 agacggagtt cgacatcgtc ggcgaactcc cgatgaagaa catcgacacc ggcatgggcc
     1081 tcgagcgcgt cgccttcctc aagcagggcg tcgagaacat gtacgagacc gaccaggtgc
     1141 gcccggtgct cgaccgcgcc gtggagctct ccggaaagaa gtacggcagc gtccacgagg
     1201 acgacgtgcg cttccgcgtg atcgccgacc acgtgcgctc ctcgctgatg ctgctctccg
     1261 acggcgtgcg cccgtccaac gaggggcgcg gctacatcct gcgccgcctg atgcgccgca
     1321 cggtgcgcgc catgcgcctg ctgggcgtcg acgagccggt cttccccgag ctcttcgcca
     1381 cgtcgcggga tgccatgaag tcggcctacc cggtgctcga gaccgagtgg gcgacgctgt
     1441 cctcggcggc cttcgccgag gaggacacct tccgccgcac gctggcgcag ggctcgacga
     1501 tcctggatct cgccctggag gacacgaaga agtccggcgg atcgacgctc agcggctccg
     1561 aggcgttcct gctgcacgac acctacggct tcccgatcga cctcaccctc gaggtcgcgg
     1621 aggaggcagg gctcgacgtc gaccgctccg cgttcgactc gctcatgaag gagcagcgcg
     1681 agcgcgccaa ggcggatgcg cgcaaccgca agcgccagct ggccgacgtc tcggtgtacc
     1741 gcgacttccg cgccgcgggc gagaccgtgt tcgccggcta caccgacctg cagaccgact
     1801 cgcgcatcct cggcatcatc gtcgacggcg cacccgtgcg ggcggcatcc gaagggcagg
     1861 tcgccgaggt catcctcgcc gagaccaccc tctacgccga gtccggcggt caggtctccg
     1921 acaaggggac gatcgtcggc accggcttcg agctggacgt cctggacgtg cagaagcccg
     1981 tccccggtct gatcagccac accgtgcacg ttgcctcggg cagcgtcgcg gtcgacgacc
     2041 gggccaccac ggtcgtcgac gccccgaacc gtcgcgccgc gcgccaggct cattcagcga
     2101 ctcacctcgt gcatgccgct ctccgcgaca cgctcggcaa gacggccaca caggccggtt
     2161 cgctgaaccg cgccggttac atgcgcttcg acttcgcgtg gtcgcagccg ctctcggccg
     2221 agacgcgttc cgagatcgag gacatcacga accgcgccgt ccaggacgcg ctcgaggtca
     2281 ccacgcgcat cgtgtcgctc gacgaggcca aggaagccgg ggccatggca ctcttcggcg
     2341 agaagtacgg cgacgtcgtg cgcatggtcg acatcggcgg tccgtggtcg cgcgagctct
     2401 gcgcgggcac gcacgtgtcc tcgagctccg agatcgggct ggtcagcgtg gtcggcgagt
     2461 cctcggtggg tgcatcgaac cgacgcatcg aggccctggt gggtcaggac gccttccggg
     2521 agctcgccgc ggagcgcacc gtcgtctcgc agctgtccgc tgctctgaag gcgccgcgtg
     2581 accagctctc cacccgtatc gaggagctgc aggcgaatct gaaggccgcc gagaagcgca
     2641 tcgcgcagtt cgaggccaag gagcgcgagg gccgtgttcc cgctcttgcc gacgccgcac
     2701 agcaggtcgg ttcgttccgt gtcgcgtcga cctcgctcgg cgagatcggc tcggccgacg
     2761 acgtgcgcac tctcgccctg agcgtgcggg agaggctcgg cacggatgcc gcagtcgtcg
     2821 ccttcggcgg cgtcgcgaac ggacggccga tcgtcgtcgt ggccacgaac gacgcggc
//
LOCUS       DATMSX010000588         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_104118,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000588 DATMSX010000000
VERSION     DATMSX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104118"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VN041_18970"
     CDS             <1..225
                     /locus_tag="VN041_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097434.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HWU31169.1"
                     /translation="QPDQQARILERVLLQSRLAEGLPIDDIAETHRERVAELISDGLV
                     DAASALRDRRVRLTLRGRLLADAVVRALTE"
     gene            306..1349
                     /gene="hrcA"
                     /locus_tag="VN041_18975"
     CDS             306..1349
                     /gene="hrcA"
                     /locus_tag="VN041_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWU31170.1"
                     /translation="MVTERGLEVLRAIVQDYVATHEPVGSKSIVERHAFGVSAATIRN
                     DMALLEDEDLIVAPHTSSGRVPTDKGYRLFVNHLAQVRPLSTGQRNAIETFLADPADL
                     DDLMARTVRVLTQLTGQVALAQYPSFARASITHIELVALAPNRMLVVLVTDAGGVSQR
                     IAPLPIDLDDADVAVLRAQLASLITGRAVGEATDRMQSVGQPDQTVTAPRLQPALITL
                     AAVISEELAEFRQERLVMAGAATLARREQDFRGSIHPLLEAIEEQVTLLRLMSEMVTD
                     EHGLATSIGTENAPFGLGEASIVASNYAAPGGTARVGVMGPTRMDYPSNLAAARAVAR
                     YLSRMLEEDGTTR"
     gene            1372..>1969
                     /locus_tag="VN041_18980"
     CDS             1372..>1969
                     /locus_tag="VN041_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWU31171.1"
                     /translation="MADHYEVLGVSRDASQDEIKKAYRKLARQLHPDVNPGEEASERF
                     KLVTHAYDVLGDEESRRRYDMGGDENGAGFGGFGGFGDIFETFFGAAGGGGRSARPRS
                     RRERGEDALVRVDLDLGDVVFGAHRDIEVDTAVLCETCQGTCCQPGTSPVTCDVCGGS
                     GHVQRQVRSLLGNVITTQPCGSCEGFGTRIESPCGGCAR"
BASE COUNT          305 a          697 c          698 g          269 t
ORIGIN      
        1 cagcccgatc agcaggcgcg catcctggag cgcgtgctgc tgcagagccg cctggccgag
       61 gggctgccga tcgacgacat cgcggagacc caccgcgagc gcgtggccga gctcatctcc
      121 gacggactcg tcgatgcggc atccgctctg cgcgatcggc gggtgcgact gaccctgcgc
      181 ggacgcctgc tggcagatgc cgtggtgcgg gcgctcacgg agtagcgggc ccgcctccgg
      241 gtaccgtgcc ggtccgacag agtaggattg gcactcgata ggcaagagtg ccagcgggag
      301 ggatgatggt cacagaacga ggactcgaag tccttcgcgc gatcgtgcag gactacgtcg
      361 cgacccacga gcccgtcggc agcaagtcga tcgtcgaacg gcacgccttc ggggtctccg
      421 ccgccacgat ccgcaacgac atggccctgc tggaggacga ggatctcatc gtcgctccgc
      481 acacctcgtc cggccgcgtg ccgacggaca agggctaccg gctgttcgtg aaccacctgg
      541 cgcaggtgcg tccgctctcc accgggcagc gcaacgcgat cgagacgttc ctcgccgacc
      601 ctgccgacct cgacgacctg atggcacgca ccgtgcgcgt gctcacccag ctgaccgggc
      661 aggtcgcgct cgcccagtac ccctcgttcg cgcgcgccag catcacgcac atcgagctgg
      721 tcgcgctcgc gccgaaccgg atgctcgtgg tgctcgtcac cgacgccggc ggagtgtcgc
      781 agcgcatcgc gcccctgccg atcgacctgg acgacgccga cgtggcggtg ctgcgcgccc
      841 agctggcctc cctcatcaca ggccgcgcgg tcggtgaggc gaccgaccgg atgcagtccg
      901 tcgggcagcc cgatcagacg gtgacggccc cacgcctgca gcccgcgctg atcacgctgg
      961 ccgcggtgat ctcggaggag ctcgccgagt tccggcagga gcggctcgtg atggcgggtg
     1021 ccgcgacgct cgcgcgtcgc gagcaggact tccgcggcag catccatccc ctgctcgaag
     1081 ccatcgagga gcaggtgacg ctgctgcggc tcatgagcga gatggtcacg gacgagcacg
     1141 gcctggccac cagcatcggc accgagaacg cgccgttcgg gctgggcgag gcctccatcg
     1201 tggccagcaa ctacgccgcc cccggcggca cggcgcgggt cggcgtgatg gggccgacgc
     1261 gcatggacta tccgagcaac ctcgcggcag cccgcgccgt ggcgcgctac ctgtcgcgca
     1321 tgctcgaaga agacggaacg acccggtgac cccggtgtca gaaaggtgat cgtggcggac
     1381 cactacgaag tcctcggggt gtcccgggac gccagccagg acgagatcaa gaaggcgtac
     1441 cgcaagctcg caaggcagct gcacccggac gtgaacccgg gtgaggaggc gtccgagcgg
     1501 ttcaagctcg tcacgcacgc ctacgacgtg ctcggcgacg aggagtcgcg ccgccgctac
     1561 gacatgggcg gcgacgagaa cggcgccggc ttcggcggtt tcggcggatt cggcgacatc
     1621 ttcgagacct tcttcggtgc cgccggcgga ggcggccgct cggcccggcc gcggtcgcgc
     1681 cgggagcgcg gtgaggatgc gctggtgcgc gtggacctcg acctcggcga cgtcgtcttc
     1741 ggcgcgcaca gggacatcga ggtggacacc gcggtgctct gcgagacctg ccagggcacg
     1801 tgctgccagc cgggcacctc gccggtcacc tgcgacgtct gcggcggctc cgggcacgtg
     1861 cagcgacagg tgcgcagcct gctcggcaac gtcatcacca cccagccctg cggctcctgc
     1921 gagggcttcg gcacgcggat cgagtcgccc tgcggcggct gcgccagat
//
LOCUS       DATMSX010000589         6102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_142045,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000589 DATMSX010000000
VERSION     DATMSX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142045"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VN041_18985"
     CDS             <1..603
                     /locus_tag="VN041_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HWU31172.1"
                     /translation="RTARRPMVETADGPRFQTSENRGAPRAWLVSGQRIKAEDRSILL
                     EVIVRVLFHTIIVVSIYLLFAGHNGPGGGFAGGLVAGMALVMRYIAGGRWELGAAAPT
                     DAGRLLGFGLILAVGAALMPMFFGMAPLESTVWFADVPLLGHLEFVSSTVFDIGVYLV
                     VIGLVLDVLRSLGAEVDRQAAERRGRFQPATERGGKGVQR"
     gene            603..1160
                     /locus_tag="VN041_18990"
     CDS             603..1160
                     /locus_tag="VN041_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HWU31173.1"
                     /translation="MDVSLTLIVIMAVLFAAGVYAMMERSLTRVLIGFLLLGNATNLL
                     LLIVMGVPGKAPFFGEEGAISDPLPQALTLTAIVITFAVSAFLLALIYRSWQLGQADT
                     VEDDVDDIALRERTDAEEELFDDESSPDEEEATTDFVGTATSPITVLHMRDHAAIDDD
                     APMDVPDPSPRSAGTADDDAEGDRS"
     gene            1157..2710
                     /locus_tag="VN041_18995"
     CDS             1157..2710
                     /locus_tag="VN041_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HWU31174.1"
                     /translation="MTSLVPLIVMLPLLGAAVTLVFGRNPRLQMLVTAATLAAVSIIA
                     AVLLVAVDAADTPFAVSVGGWPVPFGIVLYVDRLAALLVLVSSIVLLAVLLFSIGQGA
                     ADGVEETPISIFNPTYLILAAGIFDAFIAGDLFNLYVGFEILLVASYVLITLGSTESR
                     IRTGAVYIVVSLVSSILFLAAIAMIYGAVGTVNMAQVAERIAQLPPETQLVLHLMLVI
                     AFGIKAAIFPVSFWLPDSYPTAPAPVTAVFAGLLTKVGVYALIRTETQLFVSSDINTL
                     LLIVALATLVVGVLGAVAQAELKRILSFTLVSHVGYMIFGLAIATENAIGATVYYIVH
                     HIVVQTTLFLAVGLIERKAGSTSILRVSGLMKAAPLLAVLYFVPAINLGGLPPFSGFI
                     GKVALFESAAQVGTPLMIVLILGGILTSLLTLYALMRAWNLAFWREGDDLAEIEGRVG
                     YLGNAPAADVQTETRRIPAIMTVATAGMVAVTIALTVFAGPLYALCDRIGAGLLQPIT
                     LQQIDEEAG"
     gene            2707..3291
                     /locus_tag="VN041_19000"
     CDS             2707..3291
                     /locus_tag="VN041_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HWU31175.1"
                     /translation="MSPESKHLTKRGLLRDILLQLPFMLWLILIWMLLWHQFTPLALV
                     TGILVAIFVTRVFRLPTVELVGRINVWYSLVFIVRFLGAVVLGAVSVAIQVFDFRRQP
                     GAAIVEIPLRYADDIVTTHVAVTASLIPGSLVVESDRDRRILYLHVIGVRSRADVDAF
                     RDEVLRWERRIVRAVGTPEQYAALKADEKGGGAR"
     gene            3315..3560
                     /locus_tag="VN041_19005"
     CDS             3315..3560
                     /locus_tag="VN041_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HWU31176.1"
                     /translation="MVVFGLAAILSVVRIVRGPSILDRTVASDVLLTEVMCVLGADMA
                     INGHMRNVPVMLIIAAVAVFGSIAVARYVARRDNTAP"
     gene            3569..3961
                     /gene="mnhG"
                     /locus_tag="VN041_19010"
     CDS             3569..3961
                     /gene="mnhG"
                     /locus_tag="VN041_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HWU31177.1"
                     /translation="MTSFLEIALPQPIADAIVLVLVLIGAILCLSAAVGLLHFRDVPS
                     RLHAATKPQVLGLVLICLAIAVAQRTIGGMLLGLLIVIPVILLQFATAPLSAHMVGRQ
                     AYRNGTVDHQGLIVDEYADSKTTPPAAG"
     gene            complement(3992..4753)
                     /locus_tag="VN041_19015"
     CDS             complement(3992..4753)
                     /locus_tag="VN041_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWU31178.1"
                     /translation="MPERLLAPEGSRVQLGEFDHGGATLVFEDFGSGARPVVLLHGIG
                     MGRSVYIDLVQRLGGRVIGIDLPGFGEAPEPRRTLTMERHADLVAAFLRSREVADAVV
                     LGHSMGSQVAAEVAARHPDVVSALILAGPTVDSTARTIRVQFGYLLRDLIGERPTVLW
                     RGAREYLRGGPHLIRKMRATIVHEPEKAYPRVQVPTLVLRGQRDPLAPMTWCHEIVEL
                     VPDARFEEIPDHGHGTLISDVGPAAEKIRDFVDGL"
     gene            4869..5747
                     /locus_tag="VN041_19020"
     CDS             4869..5747
                     /locus_tag="VN041_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWU31179.1"
                     /translation="MSTHTRALILGGAGSAGNAWLIGIVAGLLERGVDVTDADLIVGT
                     SAGSTTAVQLVAGDPRELYEATIAPVPPRLPEGPAAGRVNGPPRVNHLERTTAIIAAS
                     VDAVDMRRRLGASALELDAADDGTAAQRWRAVAASRLPSQEWPERRIVITAVDARSGD
                     PVLFDRDSGVALADAVAASTSSGLPHRIGEDRYIDGGYRTNADNADLAAGYQRVLVLS
                     PFGGRTRSPKEWGLHLADQIAALHAAGSHVETVFPEDERLFGANAMDPSLRAPAARSG
                     HEQGVALADRIRALWA"
     gene            5891..>6102
                     /locus_tag="VN041_19025"
     CDS             5891..>6102
                     /locus_tag="VN041_19025"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31180.1"
                     /translation="MAAQPGWYPAGVPGRERWWDGLQWTAYEREVAAPVAPQTPPVQQ
                     APSTPQPTPQTLPVQQTSTPQAPAAQ"
BASE COUNT          824 a         2148 c         2130 g         1000 t
ORIGIN      
        1 cgcaccgcgc gccgtccgat ggtcgagacc gccgacggcc cgcgcttcca gacctcggag
       61 aaccgcggtg caccgcgcgc ctggctggtc agcggccagc ggatcaaggc ggaggaccgc
      121 tcgatcctgc tcgaggtcat cgtgcgggtg ctgttccaca ccatcatcgt ggtgtccatc
      181 tacctgctct tcgccgggca caacgggccg ggcggcggtt tcgctggcgg actcgtcgcc
      241 ggcatggccc tggtgatgcg gtacatcgcg ggaggccgct gggaactcgg tgctgcggct
      301 ccgacggatg ccggtcgtct gctgggcttc ggcctgatcc tggccgtggg cgcggcactc
      361 atgccgatgt tcttcggcat ggcgccgctg gagagcacgg tctggttcgc cgacgtgccc
      421 ctgctcggcc acctggagtt cgtgtcgtcg acggtcttcg acatcggcgt gtacctggtc
      481 gtgatcggcc tggtgctcga cgtgctgcgc agcctgggcg ccgaggtcga ccggcaggcc
      541 gctgagcgcc gcggccgctt ccagcccgcc accgagcgcg gcggcaaggg ggtgcagcgc
      601 tgatggacgt ctcactgacc ctgatcgtca tcatggccgt gctgttcgcc gccggcgtct
      661 acgccatgat ggagcgcagc ctgacccgcg tgctgatcgg cttcctgctg ctgggcaacg
      721 ccaccaacct gctgctgctg atcgtgatgg gcgtcccggg caaggcgccg ttcttcggcg
      781 aggagggcgc gatcagcgac ccgctgccgc aggcgctcac gctcacggcc atcgtgatca
      841 ccttcgccgt ctcggccttc ctgctggcgc tcatctaccg ctcctggcag ctgggccagg
      901 ccgacaccgt cgaggatgac gtcgacgaca tcgcgctgcg cgagcggacg gatgccgagg
      961 aggagctgtt cgacgacgag tcctccccgg acgaggagga ggcgaccacc gacttcgtcg
     1021 ggaccgccac ctcgccgatc accgtgctgc acatgcgcga tcacgcagcc atcgacgacg
     1081 atgccccgat ggacgtgccg gatccttcgc cccgatccgc gggaaccgcg gacgacgacg
     1141 ctgaagggga ccgctcgtga cctcgctcgt tcccctcatc gtgatgctgc cgctgctcgg
     1201 cgccgcggtc acgctcgtgt tcggccgcaa cccgcgcctg cagatgctgg tgacggcggc
     1261 caccctggcc gcggtctcga tcatcgccgc ggtcctgctg gtggcggtgg atgccgccga
     1321 caccccgttc gcggtgtcgg tcggcggctg gcccgtgccc ttcggcatcg tgctctacgt
     1381 cgaccggctc gccgcgctgc tggtcctcgt ctcgagcatc gtgctgctcg cggtgctgct
     1441 gttctcgatc ggtcagggtg ccgcggacgg cgtcgaggag acgccgatct cgatcttcaa
     1501 cccgacgtac ctgatcctgg cggcgggcat cttcgacgcg ttcatcgcgg gcgacctgtt
     1561 caacctgtac gtcgggttcg agatcctgct ggtcgcgtcg tacgtgctga tcacgctggg
     1621 cagcaccgaa tcgcggatcc gcaccggcgc ggtctacatc gtcgtctcgc tcgtctcgtc
     1681 gatcctgttc ctggcggcga tcgcgatgat ctacggcgcc gtcggcaccg tgaacatggc
     1741 gcaggtcgcc gagcgcatcg cacagctgcc accggagacg cagctcgtcc tgcacctgat
     1801 gctggtgatc gcgttcggca tcaaggccgc catcttcccg gtgtcgttct ggctgcccga
     1861 ctcctacccc acggcgccgg cgccggtcac cgcggtgttc gccggcctgc tgaccaaggt
     1921 cggcgtgtac gcgctgatcc gcaccgagac gcagctcttc gtgagcagcg acatcaacac
     1981 cctgctgctg atcgtggccc tggcgacgct ggtggtcggg gtgctcggcg ctgtcgcgca
     2041 ggccgagctc aagcgcatcc tgtcgttcac cctggtcagc cacgtcgggt acatgatctt
     2101 cggcctggcc atcgcgaccg agaatgcgat cggcgccacg gtgtactaca tcgtgcacca
     2161 catcgtcgtg cagaccacgc tgttcctggc cgtcggcctg atcgagcgca aggcaggaag
     2221 cacgtcgatc ctgagggtca gcggcctgat gaaggcggcg cccctgctgg ccgtgctcta
     2281 cttcgtgccg gccatcaacc tgggcggcct gccgcccttc tcgggcttca tcggcaaggt
     2341 cgcgctgttc gagtccgccg cgcaggtggg cacgcccctg atgatcgtgc tgatcctcgg
     2401 cggcatcctg acctccctgc tcacgctgta cgcgctgatg cgcgcctgga acctcgcgtt
     2461 ctggcgcgag ggcgacgacc tcgccgagat cgagggccgt gtcggctacc tgggcaatgc
     2521 gccggccgcc gacgtgcaga ccgagacccg ccgcatcccc gcgatcatga ccgtcgcgac
     2581 cgcaggaatg gtcgccgtca cgatcgccct gaccgtgttc gccggcccgc tgtacgcgct
     2641 gtgcgaccgg atcggcgcgg gcctgttgca gccgatcacc ctgcagcaga tcgatgagga
     2701 ggccggatga gccccgaatc gaagcatctg accaagcgcg gtctgctgcg ggacatcctg
     2761 ctgcagctgc cgttcatgct gtggctgatc ctgatctgga tgctgctgtg gcaccagttc
     2821 acgccactgg cgctggtcac gggcatcctg gtcgcgatct tcgtgacccg cgtgttccgc
     2881 cttccgactg tcgagctcgt cggacgcatc aacgtctggt actcgctggt gttcatcgtg
     2941 cggttcctgg gcgcggtggt gctgggcgcc gtgagtgtgg cgatccaggt gttcgacttc
     3001 cggcgccagc cgggggcggc gatcgtcgag atcccgctgc ggtacgccga cgacatcgtc
     3061 accacgcacg tcgcggtcac ggcatccctc atcccgggct cgctggtggt cgagagcgat
     3121 cgcgaccgcc gcatcctgta cctgcacgtg atcggcgtgc gcagccgcgc cgatgtcgac
     3181 gcgttccgcg acgaggtgct gcgctgggag cgccgcatcg tgcgcgccgt gggcacgccg
     3241 gagcagtacg cggctctgaa ggcggacgag aaggggggag gcgcccggtg aacgacatcc
     3301 tgttcatcgc gatcatggtc gtgttcggcc tggccgcgat cctgagtgtg gtccgcatcg
     3361 tgcgcggccc gtcgatcctc gaccgcacgg tggcctccga cgtgctgctc accgaggtga
     3421 tgtgcgtgct cggcgccgac atggccatca acgggcatat gcgcaatgtg ccggtcatgc
     3481 tgatcatcgc cgccgtcgcc gtgttcggct cgatcgcggt ggcccgctac gtcgcacgga
     3541 gggacaacac ggcaccatga ccccgaccat gacctcgttc ctcgagatcg ccctgcctca
     3601 gccgatcgcg gatgcgatcg tgctggtgct ggtgctgatc ggcgcgatcc tgtgcctgtc
     3661 ggccgccgtc gggctgctgc acttccgcga tgtgccgagc cgtctgcacg ccgccaccaa
     3721 gccgcaggtg ctcggcctgg tgctgatctg cctggcgatc gcggtcgccc agcgcaccat
     3781 cggcgggatg ctgctgggcc tgctcatcgt gatcccggtg atcctgctgc agttcgccac
     3841 ggcgccgctg tcggcgcaca tggtgggtcg tcaggcgtac cgcaacggca cggtcgacca
     3901 ccaggggctg atcgtcgacg agtacgcgga ctccaagacc accccgcccg cagcgggctg
     3961 aggcgcgcga ggccgccgca cgcggcagcg ctcagagccc gtcgacgaag tcgcggatct
     4021 tctcggccgc cgggccgacg tcgctgatca gggtgccgtg gccgtggtcg gggatctcct
     4081 cgaaccgggc atccggcacg agctccacga tctcgtggca ccaggtcatc ggcgcgagcg
     4141 ggtcgcgctg gccgcgcagc acgagcgtcg gcacctgcac gcgcgggtac gccttctcgg
     4201 gttcgtgcac gatggtcgcg cgcatcttgc ggatgaggtg cggaccgccg cgcaggtact
     4261 cgcgcgcgcc gcgccacaac acggtcggcc gttcgccgat caggtcgcgc agcaggtagc
     4321 cgaactgcac ccggatggtg cgggcggtgc tgtccaccgt ggggccggcc aggatcagcg
     4381 cggagacgac gtctggatgc cgagccgcca cctccgcggc gacctggctt cccatcgagt
     4441 ggccgagcac cacggcatcc gccacctcgc gcgagcggag gaatgccgcc accaggtcgg
     4501 cgtgacgctc catggtgagc gtccggcgcg gctcgggcgc ctcgccgaat ccaggcaggt
     4561 cgatgccgat cacgcggccg cccagacgct gcaccaagtc gatgtacacg ctgcgcccca
     4621 tgccgatgcc atgcagcagc acgacggggc gggcacccga tccgaaatcc tcgaagacga
     4681 gcgtcgcccc gccatggtcg aactcgccca gctgcacgcg actcccctcg ggtgcgagca
     4741 gacgttccgg catccgtcca cgctaccggg aacgcggcgg gaacgcgctg cacgggcccc
     4801 gttcggggcg ggatgccgat gggaggggag tgacggtctc agcccctgcg tgctagcgtc
     4861 ttccccgcat gagcacacac accagggcac tcatcctggg cggcgcgggt tcggcgggca
     4921 acgcctggct gatcggcatc gtcgcagggc tcctcgaacg cggggtcgac gtcacggacg
     4981 ccgatctcat cgtcggcacg tcggccggat cgacgaccgc cgtgcagctg gtcgccggcg
     5041 acccccgcga gctgtacgag gcgaccatcg cgcccgttcc gccccgactc ccggaggggc
     5101 cggctgcggg gcgcgtgaac gggcctccgc gcgtgaacca tctcgagcgc acgaccgcga
     5161 tcatcgctgc atccgtggat gccgtcgaca tgcgccgccg cctcggagcc tcggcgctgg
     5221 agctggatgc tgccgacgac ggaacggctg cgcagcgctg gcgcgcggtc gccgcttcgc
     5281 ggctgccgtc gcaggagtgg ccggaacggc ggatcgtgat caccgcggtg gacgcgcgct
     5341 ccggggaccc ggtgctgttc gaccgggaca gcggcgtcgc gctggcggat gccgtggccg
     5401 cgagcacgtc gagcggcctg ccgcaccgca tcggcgagga ccggtacatc gacggcggct
     5461 accgcacgaa cgcagacaac gccgacctgg cggcggggta tcagcgtgtg ctcgtgctct
     5521 cgccgttcgg tgggcgcacg cgctcgccga aggagtgggg cctgcacctc gccgatcaga
     5581 tcgcggctct gcacgccgcc ggaagccacg tcgagacggt gttcccggag gacgagcgcc
     5641 tgttcggggc gaacgcgatg gacccctcgc tgcgcgcgcc cgcggcacgc tcggggcacg
     5701 agcagggcgt ggcgctcgca gatcggatcc gcgccctctg ggcgtgaccg cttcgatccc
     5761 ggacgcacgg cgtccgtggg cggggagtag atgcgtcgtg acctactcca tcgcgacgaa
     5821 tccgggggcg cgcgggcttg tacgatcgag cccaggggga ggccgctcgg ccgatccgga
     5881 gaggacgagc atggcggcgc agccgggatg gtatccggcc ggggttcccg ggcgtgagcg
     5941 ctggtgggac ggactgcagt ggaccgcgta cgagcgcgag gtggccgcgc cggtggcgcc
     6001 gcagacgcct ccggttcagc aggcaccttc gactccgcag ccgaccccgc agacgcttcc
     6061 ggttcagcag acgtcgaccc cgcaggcgcc cgcggcgcag ca
//
LOCUS       DATMSX010000590         7335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_84812,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000590 DATMSX010000000
VERSION     DATMSX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84812"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VN041_19030"
     CDS             complement(<1..543)
                     /locus_tag="VN041_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWU31181.1"
                     /translation="MSSEAVVVDVIRTPVGRGKPGGVLSGVHPVDLAAGMLQSILERN
                     GLESGQIDDVMLGCVSQVGDQAMNIARQAVLAAGFDEHIPGTTIDRQCGSSQQAVHFA
                     AAGIAAGENDIVIVGGVESMSRVPLGSSASGGSPMSPRLSARYPRGLVNQGVSAELIA
                     QRWGFDRAALDAFAAESHARA"
     gene            612..1619
                     /locus_tag="VN041_19035"
     CDS             612..1619
                     /locus_tag="VN041_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWU31182.1"
                     /translation="MTSRSGRVPTIYDVAREAGVSHQTVSRYLRGYEGIRPATRERVQ
                     EALTRLEYRPNSAARLLRAGVVNRIGLVAFRMSAPGPARLVGGANAAARAGGYMLDIV
                     SVDEEDAESIDKAVSMLAEHQLAGVVLTAATEGLEDMLLGHGLDVPHVSGARVKLQGD
                     GMPLNEYAGRIAARHLIDLGHREIGYLSGPLSWVSARDRRAGFLTELQDHGLTVAAEA
                     EGDWLAATGAAAWSTLTAQSERLTAVGAGNDSMAVGLISAITASGRSVPGDVSVIGTD
                     DSPEAAYLNPPLSTVAMAFESEGAHLVAALLAKISGSRQRVPDVAAPELRPRSSTATP
                     R"
     gene            1727..3829
                     /locus_tag="VN041_19040"
     CDS             1727..3829
                     /locus_tag="VN041_19040"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWU31183.1"
                     /translation="MASTAPFPGILFGAAYYAEYQPDGTLDRDLDLMQEAGFTVIRVG
                     ESVWSTWEPREGEFHLDWLQPVLDGAHARGIGVILGTPTYAIPPWLQRRHPEIAAETR
                     SGERVGWGSRQEMDQSQPAYRFYAERIVRKVVERYAQHPAIIGYQVDNEPGNLLPHNA
                     ATFDTFVSWLRKRYGTVDRLNEEWGLVYWSHRISEWAELWRPDGNMMPQYQLEWRRFQ
                     ATLASDLIGWQADVVREYAHPGQFITTCISYSRPQVADDVLVEALDVTAGNPYYKMQD
                     GLDADVDASGQERWWHSGVWALHQWGDRAWSSAQAPFLVTETNAQSIGGSWQNHPPYP
                     GQLKQAAFALLSRGARMIEYWHWHTLHFGIETYWGGVLPHSQIPGRIHREVAELGSAL
                     RSLGGALDEFEPDADVVMLWSTDTKWSWDFYPPLAREDGSPDPDSYTRIFDAWYRGLF
                     ESGAQVRVHHVASFLAEDPAAFAQRHPVLVAPGLYVADDAVLDGLRAYAEAGGHLVLG
                     IRTGYGDELARARRERPPARLTDAAGVWYEEYSNRSEPLAVNAEQPFPSVLEEGAAGA
                     DWVDVLHVDDAEILLAFEPWELGTGAALTSKAFGRGRVSYLATVPNAPLARSLGRWLV
                     AHPAAAAWQAEQEVTVVTGASGTRRLAFASNWSGEASVVVAPTALTDLVDGVTHAAGS
                     RIVLGPRDARIFEVTSDR"
     gene            3863..5212
                     /locus_tag="VN041_19045"
     CDS             3863..5212
                     /locus_tag="VN041_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWU31184.1"
                     /translation="MKRSSKAMMLGGVALVSVLAIAGCTPSSPADAKKEAPVTQAQID
                     KAMNTDTDLTFWTWVPGIEKEVALFEAKYPKIHVTLVNTTGGSQHYPKLRAALKAGKG
                     APDVAQMEFQYISSFRQTGDLADLSPYGARKTQDEFVDWVWNQVADEKGVWAVPQDSG
                     PIGTLYRHDIFAAAGIDEAPATWAEFADAAAAIREKTDSYITDLPGNDPGQMVGLFWQ
                     AGAQPFGYDSGKTVTVNIDSPETQKVVDYWQDLIKRDLVATDADFNDEWYQGAARGKY
                     ASWLVAAWGPVFLQGTAKNTSGNWTAAKIPQWKEGEDVSGNWGGSTSVVLQSSQKKIA
                     AYEFAKFLNTSTESALALANDQFLFPTTKAVLNDPKFVDQTSEFYGGQKVNEFFAGVS
                     DTVSKKFEWLPFMDYVYSSYNETLGKAISDHGDMRAALTAWQDEVTSYAKQQGFTVK"
     gene            5282..6193
                     /locus_tag="VN041_19050"
     CDS             5282..6193
                     /locus_tag="VN041_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWU31185.1"
                     /translation="MSHTMNRRHARGLHSAQTRAAWMLVIPFLVVFATMFLVPLFYSA
                     YLSTFTSQLVGGEVFSAFANYSRALSDPSFWGGLSRVALFLFIQVPIMLIASLFFALA
                     LDSGRVRGGKIARLLIFLPYAVPGVVATLMWGYLYGNNFGLIAQVFRSLGLGSPELLS
                     PDKVLGSTMNIVCWEFVGYNMIILYAALRTIPRERYEAAEIDGAGQLRIAWSIKIPAV
                     KQALLLTVIFSIIGSFQLFNEPNLLYNIAPNAIGTDFTPNLYAYSVAFKNQDINYAAA
                     IAFLLGAVIVIISVVVQTIVNRKEREA"
     gene            6190..7104
                     /locus_tag="VN041_19055"
     CDS             6190..7104
                     /locus_tag="VN041_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWU31186.1"
                     /translation="MTATSTLHTAELPQRKRKRLITEDRRRSTLLTVIVWVCVAYFIV
                     PLVWLALSSTKDNTDLFTTFGLWFGNAFHLFDNIGTVFTNSGGLFARWLLNTLIYAAA
                     SALGATILAAMCGYALAKYRFRGRNAVFWLIIGAIMVPMTALAIPTYLLFSAMNLTDS
                     MWGIILPSLVSPFGVYLMRVYAQEAVDDSLLEAARIDGAGELRIFSSIGLRLLAPGLV
                     TVLLFQLVATWNNYFLPLIMLNSEDLFPITVGLANWQMAASGGGGAQVLFSTVITGSL
                     VSIAPLVIAFLFLQRYWQSGLTAGGVKG"
     gene            complement(7145..>7335)
                     /locus_tag="VN041_19060"
     CDS             complement(7145..>7335)
                     /locus_tag="VN041_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HWU31187.1"
                     /translation="VWDAGCPWVQVHTADLPDPAASRRGLAVEPMTCPPDAFNSGTDL
                     IVLEPGAAHTAGWSIRAL"
BASE COUNT         1207 a         2513 c         2408 g         1207 t
ORIGIN      
        1 ggcgcgggcg tgcgactccg ctgcgaaggc gtcgagcgcg gcgcggtcga agccccagcg
       61 ctgtgcgatc agctcggcgc tcaccccctg gttgaccagg ccccgcgggt accgggcgct
      121 cagccgtggc gacatcggcg agccgccgga tgccgaggag ccgagcggca cccggctcat
      181 ggactccact cccccgacga tcacgatgtc gttctccccg gcggcgatgc ccgcggcggc
      241 gaagtgcacg gcctgctgac tggagccgca ctgccggtcg atcgtggtgc cggggatgtg
      301 ctcgtcgaag cccgcggcca gcacggcctg ccgcgcgatg ttcatggcct ggtcgccgac
      361 ctggctcacg cagcccagca tcacgtcgtc gatctgcccg gactccaggc cgttgcgctc
      421 caggatgctc tgcagcatcc ccgcggcgag atccaccgga tgcacgcccg acagcacacc
      481 tcccggcttg ccgcgcccca ccggggtgcg gatgacatcg acgacgacgg cttcgctgct
      541 catacgtcca tcatgacgcc tcgcgaatgc gacggcgcga tcccgatcag tgcatcgcat
      601 acccttcggt tatgacaagc cgcagtggcc gcgtccccac gatctatgac gtcgcacgtg
      661 aagcaggcgt gtcccaccag accgtcagcc gctacctccg cggctatgag gggatccgtc
      721 ctgcgacgag ggagcgcgtg caggaggcgc tgacacgact cgagtaccgc cccaactcgg
      781 cggcacggct gctgcgggcc ggcgtcgtca accgcatcgg actggtcgcc ttccggatga
      841 gcgcgcccgg acccgcacgc ctggtcggcg gcgcgaacgc ggcagcgcga gccggcggct
      901 acatgctcga catcgtgtcc gtcgacgagg aggacgcgga gagcatcgac aaggccgtct
      961 ccatgctggc cgagcaccag ctcgccggag tggtgctcac cgcggcgacc gaaggtctcg
     1021 aggacatgct gctcggacac ggactcgacg ttccccacgt ctccggtgcc cgagtgaagc
     1081 tgcagggcga cgggatgcct ttgaacgagt acgcggggag gatcgcagcc cggcatctga
     1141 tcgatctggg gcatcgggag atcggctacc tgagcggccc gctcagctgg gtgtccgcac
     1201 gggatcgacg tgccggattc ctgaccgagc tgcaggatca cggtctgacg gtcgcagcgg
     1261 aggcggaggg cgactggctc gctgcgacgg gtgctgccgc gtggtccacg ctgaccgcgc
     1321 agtccgagcg cctcaccgcg gtcggagcgg ggaacgacag catggccgtc ggcctgatct
     1381 ccgcgatcac ggcgtccggg cggtcggtgc ccggcgacgt cagcgtgatc ggcaccgacg
     1441 actcgcccga ggcggcctat ctcaacccgc ccttgtcgac cgtcgcgatg gcattcgagt
     1501 ccgagggagc gcatctggtg gcagcgcttc tggccaagat ctccggaagt cggcagcgcg
     1561 tgcccgacgt cgccgccccc gagctccgtc cgcgcagctc caccgccacc ccgcgctgat
     1621 caagtcggcc ggggatgacg cacttcagac tcactcttgt tatcgacaac atttgctgct
     1681 aatttcaggt ccactgctct tgttatcgac aacattggag ttgcaggtgg cctctaccgc
     1741 gcccttcccc ggcatcctct tcggagccgc gtactacgcc gaataccagc cggacggcac
     1801 cctcgaccgc gacctcgacc tgatgcagga agcaggattc acggtcatcc gcgtcgggga
     1861 gtccgtctgg tcgacgtggg aaccgcgaga gggcgagttc cacctcgact ggctgcagcc
     1921 cgtgctcgat ggggcgcatg cccgcggcat cggagtgatc ctcggcacgc cgacctatgc
     1981 gatcccgccg tggctgcagc gacggcatcc cgagatcgct gcggagaccc ggtcggggga
     2041 gcgcgtcggc tggggatcgc gccaggagat ggaccagagt cagcccgcct accgcttcta
     2101 cgccgaacgg atcgtgcgca aggtcgtcga gcgctacgcg cagcatccgg cgatcatcgg
     2161 ctaccaggtc gacaacgagc cggggaacct cctgccgcac aacgccgcga cgttcgacac
     2221 cttcgtcagc tggcttcgaa agcggtacgg cacggtggac cggctgaacg aggagtgggg
     2281 gctcgtgtac tggtcccatc gcatcagcga gtgggcggag ctgtggcgcc cggacgggaa
     2341 catgatgccg cagtatcagc tggaatggcg caggttccag gccactctgg cgtcggatct
     2401 gatcggctgg caggccgacg tggtgcggga gtatgcgcac ccgggccagt tcatcacgac
     2461 ctgcatctcg tactcccgtc cgcaggtcgc cgacgacgtg ctggtggaag cgctcgatgt
     2521 caccgcggga aatccttact acaagatgca ggacggattg gatgccgatg tcgatgcgtc
     2581 cggccaggag cgctggtggc actccggcgt gtgggctctg catcagtggg gcgatcgtgc
     2641 gtggtccagc gcgcaggccc cgttcctcgt gaccgagacg aatgcgcagt ccatcggcgg
     2701 ctcgtggcag aaccacccgc cctatcccgg acagctcaag caggcggcgt tcgctctgct
     2761 ctcgcgcgga gcgcgcatga tcgagtactg gcactggcat acgctgcact tcggcatcga
     2821 gacgtactgg ggcggggtgc tcccgcacag ccagatcccc ggtcgcatcc atcgcgaggt
     2881 cgcggaactc gggtcggccc tgcgatcgct cggcggcgcg ctggacgagt tcgagccgga
     2941 cgccgacgtg gtgatgctgt ggtcgacgga cacgaaatgg tcgtgggact tctacccgcc
     3001 cctggcccgc gaggacggct cccccgatcc ggactcctac acacggatct tcgacgcctg
     3061 gtaccgggga ctgttcgagt ccggcgcgca ggtgagggtg caccatgtgg cgagcttcct
     3121 cgccgaggat ccggcagctt tcgcacaacg gcatccggtc ctcgtcgcac cgggactgta
     3181 cgtggccgac gatgccgtgc tggacggcct gcgggcctac gccgaggcag gcgggcacct
     3241 cgtgctcggc atccgcaccg gctacggtga cgaactggcc cgtgcgcgcc gcgagcgacc
     3301 gccggcacgg ctgacagatg cggcaggggt ctggtatgag gagtacagca accgctccga
     3361 gccgcttgcc gtgaacgccg agcagccgtt cccatccgtc ctcgaggaag gcgcagcggg
     3421 cgccgactgg gtcgatgtgc tgcacgtgga tgatgccgag atcctgctgg ccttcgagcc
     3481 gtgggaactc ggcaccggcg cggctctcac gtcgaaggcc ttcggccgcg gaagagtgag
     3541 ctacctcgcc acagtgccga atgctccact ggcacgcagc ctcggccgct ggctggtcgc
     3601 acacccggcg gccgccgcgt ggcaggccga gcaggaggtg acagtcgtca ccggcgcgtc
     3661 gggaacccga cgactggcct tcgcctccaa ctggtccggt gaggcatccg tcgtcgtggc
     3721 tcccaccgcg ctcaccgatc tcgtcgacgg cgtcacgcac gccgccggtt cccgtatcgt
     3781 cctcggcccg cgcgatgcgc ggatcttcga ggtcacctcg gaccgctgac cgcggcccgc
     3841 ccaccacagc agagagagaa ccatgaagcg ctctagcaag gcaatgatgc tcggcggcgt
     3901 cgcactggtg tcggtgctcg ccatcgccgg ctgcactccg tcttcgcccg ccgacgccaa
     3961 gaaagaggcg cccgtcaccc aggcgcagat cgacaaggcc atgaacaccg acaccgatct
     4021 gaccttctgg acctgggtgc ccggaatcga gaaggaggtc gccctcttcg aggcgaagta
     4081 tccgaagatc cacgtgacgc tcgtgaacac gaccggcggc tcccagcact atccgaagct
     4141 gcgcgccgct ctcaaggcgg gcaagggcgc accggatgtc gctcagatgg agttccagta
     4201 catctcctcg ttccggcaga ccggcgatct cgccgatctc tcgccgtacg gggcacgcaa
     4261 gacccaggac gagttcgtcg actgggtctg gaatcaggtc gccgacgaga agggcgtgtg
     4321 ggcggtgccg caggactccg gcccgatcgg aacgctgtac cggcacgaca tcttcgcggc
     4381 ggccgggatc gatgaggcgc ctgctacctg ggccgagttc gcggatgccg ccgcggccat
     4441 ccgggagaag accgactcgt acatcacaga cctgcccggc aacgacccgg ggcagatggt
     4501 cggactgttc tggcaggccg gcgcccagcc gttcggttac gacagcggca agaccgtgac
     4561 ggtgaacatc gactcccccg agactcagaa ggtcgtcgat tactggcagg acctcatcaa
     4621 gcgggacctg gtggcgacgg acgcggactt caatgacgag tggtaccagg gcgcggcccg
     4681 cgggaagtac gcgagctggc tcgtcgcggc atggggcccg gtcttcctgc agggcacggc
     4741 gaagaacacc agcggcaact ggacggccgc gaagatcccg cagtggaagg agggtgagga
     4801 tgtctccggc aactggggcg gatcgacgtc ggtcgtgctg cagtccagcc agaagaagat
     4861 cgcggcctac gagttcgcga agttcttgaa cacatcgacg gagtcggccc tcgcgctggc
     4921 gaacgaccag ttcctgttcc cgaccacgaa ggcggtgctg aacgacccca agttcgtcga
     4981 tcagacctcg gagttctacg gcgggcagaa ggtcaacgag ttcttcgcgg gcgtctccga
     5041 cacagtctcg aagaagttcg aatggctgcc cttcatggac tacgtgtact cgagctacaa
     5101 cgagacgctg ggcaaggcga tcagcgatca cggcgacatg cgcgcagcgc tgaccgcctg
     5161 gcaggacgaa gtcacgtcct acgcgaagca gcagggcttc accgtcaagt gaccggtcgg
     5221 gggcggtcgt gaaccgcccc cgacccctcg agcgcccgaa cacccacaga gagccgcagc
     5281 catgtcccac acgatgaacc ggcgccacgc caggggcctg cacagcgccc agacgcgcgc
     5341 cgcctggatg ctcgtcatcc cgttcctcgt cgtcttcgcg acgatgttcc tcgtcccgct
     5401 gttctactcc gcgtacctca gcacgttcac ctcccagctc gtcggcggag aggtcttcag
     5461 cgccttcgcc aactactcgc gggcgctgtc cgatccgagc ttctggggcg ggctgagccg
     5521 agtcgcgctc ttcctgttca tccaggtgcc catcatgctc atcgcgtcgc tgttcttcgc
     5581 attggcgctg gacagcggcc gagtccgggg cggaaagatc gcccgcctcc tgatcttcct
     5641 cccgtacgcg gtgccgggcg tcgtggcgac cctcatgtgg ggctatctgt acggcaacaa
     5701 cttcggtctc atcgcgcagg tcttccgatc gctcgggctg ggcagccccg agctgctgag
     5761 ccccgacaag gtgctcggct cgacgatgaa catcgtctgc tgggagttcg tcggctacaa
     5821 catgatcatc ctgtacgcgg cgctgcgcac catcccgcgc gaacgctacg aggctgcgga
     5881 gatcgacgga gccggccagc ttcgcatcgc ctggagcatc aagatccccg ccgtcaagca
     5941 ggcgctgctg ctcacggtga tcttctcgat catcggctcc ttccagctct tcaacgagcc
     6001 gaatctgctc tacaacatcg caccgaatgc gatcggcacc gacttcacac cgaacctgta
     6061 cgcgtacagc gtcgcgttca agaaccagga catcaactac gccgcggcga tcgcgttcct
     6121 gctcggtgcc gtcatcgtca tcatctccgt ggtcgtgcag acgatcgtca acaggaagga
     6181 gcgtgaggca tgaccgccac gtcgaccctt cacaccgctg aactgcctca gcggaagcgc
     6241 aagcgcctga tcaccgagga ccgtcgtcgc agcactctgc tcacggtgat cgtctgggtc
     6301 tgcgtcgcct acttcatcgt gccgctcgtc tggctcgcgc tctcgagcac caaggacaac
     6361 accgacctgt tcacgacctt cggcctgtgg ttcggcaacg ccttccatct cttcgacaac
     6421 atcggcaccg tgttcaccaa cagcggtggc ctgttcgcgc gctggctgct gaacaccctg
     6481 atctacgccg ccgccagcgc cctcggcgcg acgatcctgg ccgcgatgtg cggctatgca
     6541 ctcgcgaagt accgcttccg cggtcggaac gcggtgttct ggctcatcat cggagcgatc
     6601 atggtgccga tgaccgcgct cgcgatcccg acgtacctcc tgttcagcgc gatgaatctc
     6661 accgattcca tgtggggcat catcctcccc tcgctggtca gcccgttcgg cgtctacctg
     6721 atgcgcgtgt acgcgcagga ggcggtcgac gacagcctcc tcgaagcggc tcggatcgac
     6781 ggagccggcg agcttcggat cttcagctcg atcgggctcc ggctgctggc accagggctg
     6841 gtcaccgtgc tgctgttcca gctggtcgcc acctggaaca actacttcct cccgctgatc
     6901 atgctcaatt cggaggatct gttccccatc acggtgggtc tggcgaactg gcagatggcc
     6961 gcctcgggag gaggcggggc ccaggtgctc ttctcgacag tgatcaccgg ctcgctggtg
     7021 tcgatcgccc cgctggtgat cgccttcctc ttcctccagc ggtattggca gtccgggctc
     7081 accgctggcg gcgtgaaggg ctgacaccgg tcgagcggcg gccgctgccg tcgctcgacc
     7141 gcgatcagag cgcccggatg ctccagcccg ccgtgtgcgc ggcgccgggc tcgagcacga
     7201 tcaggtcggt gccggagttg aaggcgtcgg gagggcaggt catcggctcg acggcgaggc
     7261 cgcgccggct ggcggcaggg tccgggagat cggccgtgtg cacctggacc cacgggcatc
     7321 cggcgtccca cacca
//
LOCUS       DATMSX010000591         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_151283,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000591 DATMSX010000000
VERSION     DATMSX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151283"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VN041_19065"
     CDS             <1..337
                     /locus_tag="VN041_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31188.1"
                     /translation="CLTELAGPASWGALAPGRIGGPSGDELFARLFASFRANEVTSLT
                     GFRAPVRIDAGRVDEVAPAFLSARMLRTYRRAGVDLTARWWPTHHSGVMHARHAPSQA
                     ATWIAERFA"
     gene            complement(470..2620)
                     /locus_tag="VN041_19070"
     CDS             complement(470..2620)
                     /locus_tag="VN041_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWU31189.1"
                     /translation="MTNPAQNNEEQQSALEQFGINLTDRARQGKLDPVIGRDSEIRRV
                     SQVLTRRTKNNPVLIGEPGVGKTAVVEGLAQRIVAGDVAESLKDKELVSLDISALVAG
                     AMYRGQFEERLKSVLKEITESDGRVITFIDELHVLMGAGGGEGSVAASNMLKPMLARG
                     ELRMIGATTLNEYREFIEKDAALERRFQQVYVGEPSVGDTIAILRGLKGRYEAHHGVT
                     ISDSALVASAALSNRYLPSRQLPDKAIDLIDEAMSRLKMEIDSSPVEIDELKRQVDRM
                     KLEELALKREKDAASKERLATLREQLIGLEKELAALEERWARERSGLNRVGELKKQLD
                     DAITQRDLAMRNADYTKASKLEYETIKRLERDIAEAEQTEAAVSDEGRMVNEQVTDED
                     IAAVIAAWTGIPVGRLLQGESEKLLHLENELGKRLIGQKDAVKAVSDAVRRSRAGISD
                     PGRPTGSFLFLGPTGVGKTELAKALAEFLFDDEHAMVRIDMSEYGEKHSVSRLVGAPP
                     GYVGYEQGGQLTEAVRRRPYSVVLLDEVEKAHPEVFDVLLQVLDDGRLTDGQGRTVDF
                     SNVILILTSNIGSPILIDPIMSPDDKREAVMALVRQSFRPEFLNRLDDIVMFSALSQD
                     DLAQIVELSVDALQRRLGDRRLTLAVTPDARSWLAERGYDPVFGARPLRRLIQTEVQN
                     RLATALLSGSVHDGDTVRVDVAADGSGLALSTQA"
     gene            2760..>3254
                     /locus_tag="VN041_19075"
     CDS             2760..>3254
                     /locus_tag="VN041_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31190.1"
                     /translation="MSKIIGLIGMLAPLAALAVLIVHRRRAPLPAAIGMTGAILASLG
                     TVAEFLSARVAWLGGEGMHGVLERLEGWGLIRFGLVAGGLLLLLIAACLGHGRVVPRF
                     PLALAGAIAATLGIAMRLMPLDLGDLDGATRFFATMALETAQFGLLGAGTLLVAIAVV
                     SGRDD"
BASE COUNT          434 a         1118 c         1114 g          588 t
ORIGIN      
        1 ctgcctgacc gagctggcgg gccccgcgtc gtggggcgcg ctcgccccgg gacggatcgg
       61 cgggccctcc ggcgacgagc tcttcgcccg gctgttcgcc tcgttccggg cgaacgaggt
      121 cacgtctctg acgggattcc gcgcgccggt ccggatcgac gccggacggg tcgacgaggt
      181 cgccccggcg ttcctgtctg ctcggatgct gcgcacgtac cgccgcgcgg gagtcgacct
      241 caccgcgcga tggtggccga cgcaccactc gggtgtgatg cacgccaggc atgcgccctc
      301 ccaggcggcc acctggatcg cggagcggtt cgcctgacgc cggcatcccg cagctcccgc
      361 ccgacttcgg cacggatgtc ggccgagagt acggatgtcg gtcgatccag ccgatctgat
      421 ccgacatctg tgttctgagc cgacatccgt accgggacgg aagccggtgt caggcctggg
      481 tgctgagcgc cagtccggaa ccgtcggcgg ccacgtccac ccggacggtg tccccgtcgt
      541 gcacgctgcc cgagagcagc gcggtcgcga ggcggttctg cacctcggtc tggatgaggc
      601 ggcgcagcgg acgagcaccg aacaccgggt cgtagccgcg ctcggccagc caggaccggg
      661 catccggggt gaccgccagc gtgagccggc gatcgccgag gcgccgctgc agggcatcca
      721 ccgacagctc cacgatctgc gccaggtcgt cctgcgacag cgccgagaac atcacgatgt
      781 catcgagacg gttcaggaac tcggggcgga acgactgccg caccagcgcc atcacggcct
      841 cgcgcttgtc gtcgggcgac atgatggggt cgatcaggat gggcgagccg atgttgctgg
      901 tcaggatcag gatcacgttc gagaagtcga ccgtgcggcc ctgcccgtcc gtgaggcggc
      961 cgtcgtcgag cacctgcagc agcacgtcga acacctcggg gtgcgccttc tcgacctcgt
     1021 cgagcagcac caccgagtac gggcgccggc gcacggcctc ggtcagttga ccgccctgct
     1081 cgtagccgac gtatcccgga ggggcgccga ccagccgcga gacggagtgc ttctcgccgt
     1141 actccgacat gtcgatgcgc accatggcgt gctcgtcgtc gaagaggaac tcggcgagcg
     1201 ccttggcgag ctcggtcttg ccgacgccgg tggggccgag gaataggaag gatccggtgg
     1261 ggcgaccggg gtcgctgatc ccggcgcgcg agcgtcgcac ggcatccgac accgccttga
     1321 cggcgtcctt ctgcccgatc agacgcttgc ccagctcgtt ctccaggtgc agcagcttct
     1381 cgctctcgcc ctgcagcagc cggccgacgg ggatgccggt ccatgcggcg atcacggcgg
     1441 cgatgtcctc gtcggtgacc tgctcgttga ccatccggcc ctcgtcggag acggcggcct
     1501 cggtctgctc ggcctcggcg atgtcgcgct ccaggcgctt gatcgtctcg tactcgagct
     1561 tggacgcctt ggtgtagtcg gcgttgcgca tggcgaggtc gcgctgcgtg atggcgtcgt
     1621 cgagctgctt cttcagctca cccacccggt tcagccccga ccgctcgcgg gcccagcgct
     1681 cctcgagggc ggcgagctcc ttctcgaggc cgatcagctg ctcgcgcagg gtggccaggc
     1741 gctccttgga ggcggcgtcc ttctcgcgct tgagggcgag ctcttcgagc ttcatccggt
     1801 cgacctggcg cttgagctca tcgatctcca ccggcgagga gtcgatctcc atcttcagcc
     1861 gcgacatggc ctcgtcgatc aggtcgatgg ccttgtcggg cagctgccgg ctgggcaggt
     1921 agcggttgga gagcgcggcg gatgccacca gggcactgtc ggagatcgtc accccgtggt
     1981 gggcctcgta gcgtcccttg agtccgcgca ggatggcgat ggtgtcgccg accgagggct
     2041 cgccgacgta cacctgctgg aagcggcgct ccagcgcagc atccttctcg atgaactccc
     2101 ggtactcgtt gagcgtggtc gcgccgatca tgcgcagctc gccgcgggcg agcatcggct
     2161 tgagcatgtt ggatgccgcg accgacccct cgccgccgcc ggcgcccatg agcacgtgca
     2221 gctcgtcgat gaacgtgatg accctgccgt cggattcggt gatctccttg agcacgctct
     2281 tcaggcgctc ctcgaactga ccgcggtaca tggcgccggc gacgagggcg gagatgtcga
     2341 gactcaccag ttccttgtcc ttgagggatt cggcgacgtc tcccgcgacg atgcgctgag
     2401 cgagcccttc gaccacggcg gtcttgccca cgccgggctc cccgatcagg acggggttgt
     2461 tcttggtgcg ccgggtgagc acctggctca cacggcggat ctcgctgtca cggccgatca
     2521 cgggatcgag cttgccctgg cgggcgcgat ccgtgaggtt gatgccgaac tgctcgaggg
     2581 cgctctgctg ctcttcgttg ttctgtgcgg ggttggtcat tcgttcctcc tgaggagcct
     2641 ctccttcaaa gttgagtggt gatgactcaa gtttagccac agttcccctc ccgggcaaag
     2701 ccttcctttt ttggacatac agtcgtatgg ttttggttat acagacgtat ggaggcactg
     2761 tgtcgaagat catcgggctc atcgggatgc tcgctccgct cgccgccctg gccgtgctga
     2821 tcgtgcaccg ccgtcgcgct cctctcccgg ccgcgatcgg gatgaccggc gcgatactgg
     2881 cgagcctggg caccgtcgcc gagttcctca gtgcacgcgt ggcctggctc ggaggagaag
     2941 ggatgcacgg cgtgctcgag cggctggagg gctggggcct cattcgcttc ggcctggtgg
     3001 cgggcggcct tctcctgctg ctgatcgccg cctgtctcgg ccacggtcgc gttgttccgc
     3061 gattcccgct cgcgctcgcc ggggcgatcg ccgccaccct cggcatcgcg atgcgcctca
     3121 tgccgctcga cctcggcgac ctcgacggcg ccacgcgctt cttcgccacg atggccctcg
     3181 agaccgccca gttcggcctg ctcggcgccg ggaccctgct cgtggcgatc gccgtcgtca
     3241 gcggtcgcga cgac
//
LOCUS       DATMSX010000592         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_113682,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000592 DATMSX010000000
VERSION     DATMSX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113682"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VN041_19080"
     CDS             <1..823
                     /locus_tag="VN041_19080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWU31191.1"
                     /translation="PGGGSKQVIVAGQPLDDLHDEDCMLFSFMDMEPRRKAETALRQS
                     EERFAKSFRLTPVPTLVCSAEQQLLEINEAFLQTSGYASEELLGKTVEEIGFLDADAC
                     AALFATLEKTATVSSVDVKVHKKGGEQIDCEMAADTVVIQDKPCYLLVLMNITERKRS
                     ELELVAAIEEVMKDASWFSRTLIEKLANVKSINAQLPSTSFTELTARERDVLGLICQG
                     LADKEIAARLKLAPNTVRNHVATLYSKLDVHSRSEAIVWARERGLFANEWRMKAP"
     gene            836..1303
                     /locus_tag="VN041_19085"
     CDS             836..1303
                     /locus_tag="VN041_19085"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWU31192.1"
                     /translation="MEQVRARMVSLGMPRSAVLDHFRDKRGPDDAPHVAVGTTREFAH
                     LFAALVNASVVDAAVSAQVSEWLSLNQDLSLVAAATGLDPFAHDHDAHGLLFINKTGR
                     DRGVRAEAGVLAGPRAGIAYALTVCFDDLSISHRLRAHDAFRILGTDLMEYTH"
     gene            complement(1354..2007)
                     /locus_tag="VN041_19090"
     CDS             complement(1354..2007)
                     /locus_tag="VN041_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWU31193.1"
                     /translation="MIRVLIADDEAMILAGVRAILESDSEIEIVAEAADGREAIIAAQ
                     RHRPDVAVLDIRMPGLDGLSAAEEIIATLPGSRVVMLTTFDEDDYIERALGGGALGFL
                     LKAADPRELIAGVRAVADGGAYLSPAIARRVIAPYREGTRTGVVEARQAVAVLTDRER
                     EVLSLLGGGASNAEIGGRLHLVEGTVKGHVSAILLKLGVRNRVEAAVLAHRSGLIKP"
     gene            complement(2004..3419)
                     /locus_tag="VN041_19095"
     CDS             complement(2004..3419)
                     /locus_tag="VN041_19095"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWU31194.1"
                     /translation="MSRRADREHRMPTALGVLLLTFASATALPVARWGDSALVLMLIP
                     AALIGYLAGRRMLRLAPAVITFAVVLVLALPASYALPDGAPVGDWMAAVLLALLTVVL
                     PWWWGRYRLLRAQLRASDAIVLAERAQAEERARIADDLHDTIGHELALIAVQAGALEV
                     GPDLQPRQAVQFRELREAAVRASAHLREVVQLADQGGPARLEPAGGDLDALVDGARGA
                     GMSVETDIERGAIDRVHPLIADLAVRAVREGLTNAARHAGGSAVQVRVASADAGGMVT
                     TVGSDAAPLSGRAASASTGTRGSGHGIASLRRRAELLGAALAAGPGEDGGFLLELRAP
                     ASPQPAAATTTADDPELESNARAAHRSMLRTALVPVGVVAVGFVVFLVAQAMTSSQTA
                     LSSTTYARLELGMTQTELVHLLPAGISRPAPTVDEPAAPLGADCDYYEARDGWLHFTD
                     AAYRLCFRDGVLVEKLRLEDS"
     gene            3528..4187
                     /locus_tag="VN041_19100"
     CDS             3528..4187
                     /locus_tag="VN041_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWU31195.1"
                     /translation="MDLLALLTDAVTDLAASPWLYLVVFLVCLIDGFFPPVPSETVVV
                     GAAAIAATTGSPSLPLLIGIAAGGAIAGDNTAYLLGRLVGTTRFRWMRVGRVAATLDW
                     ARRGLDRRGALLIFTARYIPVGRIAVNMTAGATGYPPRRFVPLSIVAGLAWALYSALF
                     GLVAGQWLHEQPLLAVVIAVVVAAGVGAAVDALLRRLLASERHGEGEEQHAPTGANVE
                     A"
     gene            4184..>4517
                     /locus_tag="VN041_19105"
     CDS             4184..>4517
                     /locus_tag="VN041_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWU31196.1"
                     /translation="MIPDDWTPHRRDDGELLGWIRPDGEDWVAIDVLGRTASAPVDWL
                     DAEAALERVGLAWLADIWMLEGAAPEPLRVRFVEVTPSEGAAAGRVIVKVDDFGDMQR
                     PPAERFTLP"
BASE COUNT          731 a         1550 c         1535 g          701 t
ORIGIN      
        1 gccagggggc ggcagcaagc aagtgattgt cgcgggccag ccgctggatg acctgcatga
       61 cgaagactgc atgctgtttt ccttcatgga catggagccg cgccgcaagg ccgaaacggc
      121 gttgcgccag agtgaggagc gctttgccaa atcgttccgt ctcacgcccg tgccaaccct
      181 ggtgtgcagc gccgaacagc aattgctgga gatcaacgaa gcctttctcc agaccagcgg
      241 ttacgccagt gaagaactgc tgggcaaaac cgtggaggaa atcggctttc tcgacgcgga
      301 tgcctgcgcc gcactgtttg ccacgctgga aaaaaccgca accgtgtcca gcgtcgacgt
      361 caaggttcac aaaaaaggtg gggaacagat cgactgcgag atggcagccg atactgtcgt
      421 cattcaggac aagccgtgtt atttgctggt gttgatgaac atcaccgaac gcaaacgctc
      481 ggagctggag ctggtggcgg ccatcgaaga ggtgatgaaa gatgcctcct ggttcagtcg
      541 gaccctgatc gaaaaactgg ccaatgtgaa gagcatcaac gcccagttgc ccagcacttc
      601 atttaccgag ctcacagccc gggagcgtga tgtgctgggg ctgatctgcc agggcctggc
      661 cgacaaggaa atcgccgcgc gcttgaagct ggcccccaat accgtgcgca atcacgtggc
      721 gacgctgtac tccaaactcg atgttcacag ccgcagcgag gcaatcgtct gggcgcgcga
      781 acgcggcctg ttcgccaacg agtggcgaat gaaggcacca tgatagccag tgcaaatgga
      841 gcaggtgcgc gcccgcatgg tgagcctcgg catgccgcgc agcgccgtgc tcgaccactt
      901 ccgcgacaag cgcggtcccg acgacgcccc gcatgtggcg gtcggcacca cccgggagtt
      961 cgcccacctg ttcgccgctc tcgtgaacgc gtccgtcgtc gacgcggcgg tcagcgcgca
     1021 ggtctcggag tggctgagcc tgaaccagga cctcagcctc gtcgccgcgg cgaccggact
     1081 cgatccgttc gcgcacgatc acgacgcgca cggcctgctg ttcatcaaca agaccggccg
     1141 cgaccgcggc gtgcgcgccg aggccggagt gctcgcgggc ccgcgagcgg gcatcgccta
     1201 cgcgctgacc gtgtgcttcg acgacctgtc gatcagccac cgcctgcgcg cgcacgacgc
     1261 tttccgcatc ctcggcacgg atctcatgga gtacacgcac tgagatcgct gcccgcagcc
     1321 tgagcgcgga tggcctggcg cgcatgcgag cggtcagggc ttgatgaggc cggaacggtg
     1381 cgcgagcacc gccgcctcca cgcgatttcg caccccgagt ttgagcagga tcgcactgac
     1441 gtgccccttc accgtccctt cgaccaggtg cagccggcct ccgatctcgg cgttcgaggc
     1501 tccaccaccc agcagcgaca gcacctcgcg ctcccgatcg gtcaacacgg cgaccgcctg
     1561 tcgcgcttcg acgacgcccg tccgggtgcc ctcgcggtac ggtgcgatca cccgccgcgc
     1621 gatggcggga gacagatatg cgccgccgtc ggccacggcg cgcacgcccg cgatgagttc
     1681 acgggggtcg gcggctttca gcaggaagcc gagcgcccca ccgccgagcg ctcgctcgat
     1741 gtaatcgtcc tcgtcgaatg tggtcagcat cacgacccgg ctgccgggaa gcgttgcgat
     1801 gatctcctcc gctgcgctca gcccgtccag tccgggcatc cggatgtcga gcaccgcgac
     1861 gtcgggtctg tgtcgctggg cggcgatgat cgcctccctc ccatcggcgg cttcggcgac
     1921 gatctcgatc tcggaatcgg actccaggat cgcgcgcacg cccgcgagga tcatcgcctc
     1981 atcatcggcg atcagcaccc ggatcatgaa tcctccagac gcagcttctc gaccagcaca
     2041 ccgtcccgga agcacaagcg gtaggccgcg tcggtgaagt gcagccagcc atcacgcgcc
     2101 tcgtagtagt cgcagtctgc gcccagcggg gccgcgggct cgtccaccgt gggtgcaggg
     2161 cgcgagatac ctgcgggcag caggtggacg agttcggtct gcgtcatgcc gagctccagc
     2221 cgcgcgtatg tcgtcgacga gagtgcggtc tgggacgacg tcatcgcctg cgcgaccagg
     2281 aacacgacga acccgacggc gacgacgccg accggtacca aggcggtgcg cagcatgctg
     2341 cggtgtgcag cgcgcgcgtt ggactccagc tcggggtcgt ctgcggtggt ggtcgcagct
     2401 gcaggctgcg gggaggccgg agcgcgcagc tccagcagga aacctccgtc ctcgcccgga
     2461 cccgcggcga gtgcggcgcc gagcagttcc gcgcgtcggc gcagactcgc tatgccgtgc
     2521 ccgctcccgc gggttccggt gctggcggat gccgctctgc cggagagcgg tgcagcatcc
     2581 gagccgacag tggtgaccat gccaccggca tccgcggacg cgacccgcac ctgcacggcg
     2641 gagccgccgg cgtgacgtgc ggcgttggtg agaccttccc gcactgcgcg cacggcgaga
     2701 tcggcgatca ggggatgaac gcgatcgatc gcgccccgtt cgatgtctgt ctcgacgctc
     2761 ataccggcac cgcgcgctcc gtcgacgagg gcgtcgagat cgccgccggc cggctccaac
     2821 cgggccgggc cgccctggtc tgcgagctga acgacttcgc gcaggtgtgc actggcccgg
     2881 acggcggcct cgcgcagttc gcggaactgc accgcctggc gtggctgcag gtccggtccc
     2941 acttcgagcg caccagcctg cacagcgatc agtgcgagtt cgtggccgat ggtgtcgtgc
     3001 aggtcgtcgg cgatacgggc gcgctcctcc gcctgtgccc gctcagccag cacgatcgca
     3061 tccgaggcac gcagctgcgc gcgcagcaac cggtaccgac cccaccacca gggcagcacg
     3121 accgtgagca gcgccagcag cactgctgcc atccaatcgc cgaccggtgc gccatccggc
     3181 agggcgtatg acgctggaag tgcgagtacc agcaccactg cgaaggtgat cacggcgggt
     3241 gcgaggcgca gcatccgccg tccggcgagg tatccgatca gcgccgccgg gatcagcatc
     3301 agcacgagcg ccgagtcgcc ccagcgggct accggcagcg cggtcgccga cgcgaatgtc
     3361 agcagcagga cgccgagcgc ggtcggcatc cggtgctctc gatcagcgcg acgcgacatg
     3421 ctacccatcc taagaagcgg gatggccgtg cagacctggc gaaagtcagg gatgctgctg
     3481 gcttccgtca gatgtgcggg cccgcgaccg tccgtagcgt cgcagccatg gaccttctcg
     3541 ccctgctcac ggacgcggtc accgacctcg ctgcgtcgcc ctggctgtac ctcgtggtgt
     3601 tcctcgtctg cctgatcgac ggcttcttcc cgcccgtgcc cagcgagacc gtggtcgtcg
     3661 gtgccgcggc gatcgccgcg acgacaggtt cgccgagcct gcccctgctg atcgggatcg
     3721 ccgccggggg agcgattgcg ggagacaaca ccgcctatct gctgggtcga ctcgtgggca
     3781 ccacccggtt ccgctggatg cgcgtcggac gggtcgcggc cacgctggac tgggcgcgcc
     3841 gcgggctgga ccggcgcggg gccctgctga tcttcaccgc ccggtacatc cccgtcggcc
     3901 gcatcgccgt gaacatgaca gcgggcgcca ctggatatcc gccgcgtcgc ttcgtaccgc
     3961 tgtcgatcgt ggcgggcctg gcatgggcgc tgtactccgc attgttcgga ctggtggccg
     4021 ggcagtggct gcatgagcag ccgcttctcg ccgtcgtgat cgcggtggtg gtcgccgctg
     4081 gagtcggggc ggctgtggat gcgctgctgc gacgcctcct cgcgtcggaa cgacacggtg
     4141 aaggggagga gcagcatgcg ccgacggggg ctaacgtcga agcatgattc ccgacgactg
     4201 gaccccgcac cgccgcgacg acggagaact gctcggctgg atccgccccg acggcgagga
     4261 ctgggtcgcg atcgacgtgc tcggccgcac ggcatccgcg cccgtcgact ggctcgatgc
     4321 cgaggcagcg ctggaacggg tcgggctggc ctggctcgcc gacatctgga tgctggaggg
     4381 ggcggcgccc gagccgctgc gcgtgcggtt cgtcgaggtg acgccttcgg agggggcggc
     4441 cgccggacga gtcatcgtca aggtcgacga cttcggcgac atgcagcgtc cacccgccga
     4501 gcgcttcacg ctgccct
//
LOCUS       DATMSX010000593         6118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_170235,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000593 DATMSX010000000
VERSION     DATMSX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170235"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /gene="pheT"
                     /locus_tag="VN041_19110"
     CDS             complement(<1..1938)
                     /gene="pheT"
                     /locus_tag="VN041_19110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWU31197.1"
                     /translation="MRVPISWLREYVDVAADATPEDVLASFVSVGFEEEDIHRFEISG
                     PVVVGRVLSAEPEPQSNGKTINWCQVDVGEANGGVRGIVCGAHNFVAGDKVVVTLPGA
                     VLPGPFPIAARKTYGHVSDGMIASARELGLGDEHDGIIVLSTLGLDPEVGADAIELLH
                     LNDFAVDINVTPDRGYAFSLRGVAREYSHATGAAFRDPAERDFAELQPGSGFSISVDD
                     QSPIRGNVGAREFVARVVRNVDPSRPTPPWMIARLVLAGMRSLGVLIDITNYVMLELG
                     NPVHGYDLDKLQGGIVVRRAEQGERMTTLDGQDRALHTEDLLITDGSGPIGLAGVMGG
                     GTTEMSATTRNVLIEAATFDPVTIARSARRHKLPSEASKRFERGVDPLIPFVAARRVA
                     DLMVELAGGELTDEGGALFADVDIAGIDLPRGFAQGVIGVDYTDDEVTGALEMIGGEV
                     TATDGGWFVIPPSWRPDLTDKWTLVEEVARIHGLDRIPSVLPTPPSGRGYTPLQQGRK
                     RVADAVAAAGFVETPAFPFTTEEQNDLHGSASGEHLPSIRLANALDGQAPYLRRSLLP
                     GLLQTAHRNISRGLVDLAIFETGLVFTPEAGVTYGTDFVPPLAQRPSDETLAQLNAAI
                     PPQPRNIAVLLTGNVTPRQPEI"
     gene            complement(1938..2987)
                     /gene="pheS"
                     /locus_tag="VN041_19115"
     CDS             complement(1938..2987)
                     /gene="pheS"
                     /locus_tag="VN041_19115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWU31198.1"
                     /translation="MSDTPETPQITPEAVEYAVDAALAAIAAASDTAELKAARAAHVA
                     EGSPLAVLNASMRQVAPEHKAAFGKLVGQGRGRVTQALAAKETELAEAETAARLEAER
                     VDITAIASHARVGARHPISLMQEQISDIFVGMGWEIAEGPELEHEWFNFDALNFDEDH
                     PARQEQDTFYVDPSSRHLVMRTHTSPVQVRSMLDREVPIYVLCPGRVYRTDEFDATHL
                     PVFTQFEGLVVDKGITMAHLKGTLDHVAKQMFGAEAKTRFRTNYFPFTEPSAELDLWH
                     PTFKGGARWIEWGGCGMVNPNVLRAAGIDPEVYSGFAFGMGVERAVMFRSDVKDMRDM
                     AEGDVRFSEQFGMVV"
     gene            complement(3052..4455)
                     /locus_tag="VN041_19120"
     CDS             complement(3052..4455)
                     /locus_tag="VN041_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HWU31199.1"
                     /translation="MQLLAQTAPLLNGVLAADPVKPAAGTAQLIIAAVVSIAVIVVLI
                     VWVKMHPFFSLMLGAAVMALAANMGFEHAFTSFSTGLGSTVGGVGVLIVLGAVIGRFL
                     TESGGADEIVDTILSKTPLKRLGWAMALAAFIVGIPLFFEVGVVLLIPIVMLAAKRAK
                     LPVILIGIPALAGLSALHGFVPPHPGPLIAIDALKADLGLTLGLGLLVAIPTVIIAGP
                     VLAKPMARWVPVLASDKLLAPVTEREGKRPAFGVALSVVLLPVVLMLLMTVVTSAGLD
                     SSLFGQILVWIGTPLQALLITSLYAMIVLGTALGRSMGEVAKVTGDSFAPIASILLIV
                     GAGGGFKQTLVDSGVGQVIGGGIAGSGMPLLLAGWLVAVVIRLATGSATVATITAAGI
                     MAPLAGDLSGTHLALMVLAIGAGSVFLSHVNDAGFWLVKEYFGLTVGQTFKTWSLMEC
                     AVSVVGLLGVLLLGLVV"
     gene            4568..6073
                     /locus_tag="VN041_19125"
     CDS             4568..6073
                     /locus_tag="VN041_19125"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HWU31200.1"
                     /translation="MAEEPIPTTQAIALVSAPAPYVLAIDIGSGSTRGCLYDAYARPI
                     KKHLVKAEHAFIEAADGTAEVDADQMVREVGEVIDGALKGIDPGLVKAVVMDTFASSL
                     VCVDAEGDALTPCITYADSRSSEHLAKLRSQLDEDEVHQRTGTRLHTSYHPPRLLWLQ
                     DEFPEVFAKTAKFLSLGEYVYAKLAGIEGAATSTMAWAGILNRHTCELDEELLAAVGV
                     DRSRFAPVVDPSEPITDVSAEALRRWPALEGAAWFPAVPDGYASNIGVGASTPSVAAM
                     SAATSGAIRVIVDGTPDVLPTGLWAYRVSKTQSIVGGALNDVGRVTLWLQSTLAPLTY
                     EQIDEVFRAAPSEGTPLVLPFLTGERATGWAGDARAVLTGVSSASGPRELWRGAVEGI
                     AISYARVFEQLLEVAPGIRSVIASGGVTSAYPGFMHPVAQALGFPVEVVQVKRVTMRG
                     AAALALSVLQAEHPLATIPQTSLTEPDAAQGDYYDDLRRRFENVYQGVIAK"
BASE COUNT          977 a         2093 c         2167 g          881 t
ORIGIN      
        1 gatctcgggc tggcgaggcg tgacgttgcc ggtcagcagc accgcgatgt tccgcggctg
       61 cggcggaatg gccgcgttca gctgcgccag ggtctcatcg ctcgggcgct gcgcgagcgg
      121 cgggacgaag tcggtgccgt aggtcacgcc cgcctcgggg gtgaacacga gtcccgtctc
      181 gaagatcgcc aggtccacca gaccgcgcga gatgttgcgg tgcgcggtct gcagcaggcc
      241 gggcagcagc gagcgacgca ggtacggggc ctgcccgtcc agggcattgg ccaggcggat
      301 gctgggcagg tgctcaccac tggcggagcc gtgcagatcg ttctgctcct cggtggtgaa
      361 cgggaacgcc ggcgtctcga cgaagcccgc agcggcgacg gcgtcagcca cgcgcttgcg
      421 accctgctgc agcggggtgt agccacgacc ggagggcggg gtcggcagga cggacgggat
      481 gcggtcgaga ccgtggatgc gggcgacctc ctccacgagc gtccacttgt cggtgaggtc
      541 gggacgccag ctcggcggga tgacgaacca gccgccatcg gtcgcggtca cctcgccacc
      601 gatcatctcc agcgcgccgg tcacctcgtc gtcggtgtag tcgacgccga tcacgccctg
      661 tgcgaagcct cggggcagat cgatgcccgc gatgtcgacg tcggcgaaca gcgcgccgcc
      721 ctcgtcggtc agctcgccgc cggcgagctc gaccatcaga tcggccacgc ggcgggccgc
      781 gacgaacggg atgagagggt cgacgccacg ttcgaagcgc ttggatgcct cgctgggcag
      841 cttgtggcgt cgtgcggagc gtgcgatcgt gaccgggtcg aaggtcgcgg cctcgatcag
      901 aacgttgcgc gtggtcgcgc tcatctcggt ggtgccaccg cccatcacgc ctgccagacc
      961 gatcggtccc gagccgtcgg tgatcagcag gtcctcggtg tgcagcgcgc ggtcctgccc
     1021 gtcgagcgtg gtcatccgct cgccctgttc ggcgcggcgc acgacgatgc cgccctgcag
     1081 cttgtcgagg tcgtagccgt gcacggggtt gccgagctcg agcatcacgt agttggtgat
     1141 gtcgatgagc acgcccagcg agcgcatgcc ggccaggacc agccgcgcga tcatccacgg
     1201 cggggtcggc cgcgacgggt cgacgttccg cacgacgcgg gcgacgaact cgcgggcacc
     1261 gacgttgccg cggatggggc tctggtcatc gaccgagatc gagaacccgg agccaggctg
     1321 cagctcggcg aagtcgcgct ctgcggggtc gcggaaggcg gccccggtgg cgtgcgagta
     1381 ctcacgggcg acgccgcgca gcgagaacgc gtagccgcgg tcgggcgtca cgttgatgtc
     1441 gaccgcgaaa tcgttcaggt gcagcagctc gatggcatcc gccccgacct cggggtcgag
     1501 cccgagcgtc gacagcacga tgatgccgtc gtgctcatcg cccaggccca gctcacgcgc
     1561 cgaggcgatc atgccgtcgg agacgtggcc gtaggtcttg cgagccgcga tggggaacgg
     1621 gccgggcagc acggcaccgg gcagggtcac cacgaccttg tcaccggcga cgaagttgtg
     1681 cgcgccgcag acgatgccac gcacgccgcc gttcgcctcg ccgacgtcca cctggcacca
     1741 gttgatggtc ttgccgttgg actgcggctc gggctcggcc gagagcacgc gcccgaccac
     1801 gacgggaccg gagatctcga agcggtggat gtcctcctcc tcgaaaccca ccgacacgaa
     1861 ggacgccagg acgtcctccg gcgtggcgtc cgccgccacg tcgacgtact cgcgcagcca
     1921 ggagatcgga acgcgcatca caccaccatc ccgaactgct cgctgaagcg cacatcgccc
     1981 tcggccatgt cgcgcatgtc cttcacatcg ctgcggaaca tcaccgcccg ctcgacgccc
     2041 atgccgaacg cgaaaccgct gtacacctcg gggtcgatgc ccgccgcgcg cagcacgttc
     2101 gggttgacca tgccgcagcc gccccactcg atccagcgcg cgccgccctt gaaggtcggg
     2161 tgccacaggt cgagctcggc ggagggctcg gtgaacggga agtagttggt gcggaaccgg
     2221 gtcttcgcct cggcgccgaa catctgcttc gcgacgtggt cgagggtgcc cttgaggtgc
     2281 gccatcgtga tgcccttgtc gacgaccaga ccctcgaact gggtgaacac cggcaggtgc
     2341 gtcgcatcga actcgtcggt gcggtacaca cggccagggc acagcacgta gatcggcacc
     2401 tcgcggtcga gcatcgaccg cacctgcacg gggctggtgt gcgtgcgcat caccaggtgg
     2461 cgggagctcg ggtcgacgta gaacgtgtcc tgctcctgac gggcggggtg gtcctcgtcg
     2521 aagttcagcg cgtcgaagtt gaaccactcg tgctcgagct ccggcccctc cgcgatctcc
     2581 cagcccatgc cgacgaagat gtcgctgatc tgctcctgca tgagggagat cggatgccgt
     2641 gcaccgacgc gggcgtgcga ggcgatggcc gtgatgtcga cgcgctccgc ctccaggcgg
     2701 gcggcggtct cagcctcggc cagctcggtc tccttggcgg cgagcgcctg ggtcacacgg
     2761 cccctcccct gcccgacgag ctttccgaac gcggccttgt gctcgggggc gacctggcgc
     2821 atcgacgcgt tcagcacggc gagcggcgag ccctcggcga cgtgcgcggc gcgagccgcc
     2881 ttcagctcgg cggtatcgga tgccgcggcg atcgccgcga gcgcggcgtc gacggcgtac
     2941 tccaccgcct ccggggtgat ctggggtgtt tcgggagtgt ctgacacgag aaccgagtct
     3001 accggcgcga gggcggggcc cccgcgccgg tgaagaccgt tcctgcgagg ctcagaccac
     3061 cagtccgagc agcagaacgc cgagcaggcc gaccaccgag accgcgcact ccatgaggct
     3121 ccaggtcttg aaggtctgtc cgaccgtgag tccgaagtac tccttcacca gccagaagcc
     3181 ggcgtcgttc acgtgggaga ggaacaccga acccgcaccg atcgcgagca ccatcagtgc
     3241 caggtgggtt ccgctgagat caccggccag cggcgccatg atccccgccg cggtgatggt
     3301 ggccacggtc gccgagccgg tcgccaggcg gatgaccacc gcgaccagcc agccggcgag
     3361 cagcagcggc atgcccgagc ctgcgatgcc gccgccgatg acctggccga cgcctgagtc
     3421 gacgagcgtc tgcttgaagc cgccgcccgc gcccacgatc agcaggatgg aggcgatcgg
     3481 ggcgaacgag tcgcccgtga ccttcgcgac ctcgcccatg gagcgtccga gggccgtgcc
     3541 gagcacgatc atcgcgtaca gcgaggtgat cagcagcgcc tggagcggtg tgccgatcca
     3601 gaccaggatc tgaccgaaca ggctgctgtc caggcccgcg gacgtgacga ccgtcatcag
     3661 cagcatcagc acgaccggca gcagcacgac ggagagcgcc acgccgaacg cggggcgctt
     3721 cccctcgcgc tcggtgaccg gggcgagcag cttgtcggat gccaggacgg gcacccagcg
     3781 ggccatgggc ttcgcgagca cggggccggc gatgatgaca gtcgggatcg cgacaagcag
     3841 ccccaggccc agggtgaggc cgaggtcggc cttgagagcg tcgatcgcga tcagcggacc
     3901 gggatgcggc ggcacgaagc cgtgcagcgc cgacagacct gccagcgcgg ggatgccgat
     3961 caggatcacg gggagcttgg cgcgcttggc cgcgagcatg acgatgggga tcagcagcac
     4021 cacgccgacc tcgaagaaca gcgggatgcc gacgatgaac gcggcgagcg ccatggccca
     4081 gcccaggcgc ttgaggggcg tcttcgacag gatcgtgtcc acgatctcgt cggcgccccc
     4141 ggactcggtg aggaaacgtc cgatcacggc gccgagcacg atcagcacgc cgacgccgcc
     4201 gacggtggag cccagtcctg tggagaagga ggtgaaggcg tgctcgaagc ccatgttcgc
     4261 ggcgagcgcc atgacggcgg cgcccagcat cagggagaag aacgggtgca tcttcaccca
     4321 gacgatcagc acgacgatga ccgcgatcga gaccaccgcc gcgatgatca gctgagcggt
     4381 gcccgccgcg ggcttgacgg gatccgcggc aagcaccccg ttcaggagtg gtgccgtctg
     4441 tgcgagcagt tgcattctcg gactccttcg ctgcgtcggg gcggggccgt cccggaacgt
     4501 agcgcacacg atactcccag gtaggatcgg cgacgagaac gactcacacg cgcgagaggc
     4561 gaattccatg gccgaagaac cgataccgac gacgcaggcc atcgccctgg tatccgcacc
     4621 agcgccttat gtgctcgcga tcgacatcgg ctcgggttcg acacgtgggt gcctctacga
     4681 cgcctatgcc cgtccgatca agaagcacct ggtcaaggcc gagcacgcct tcatcgaggc
     4741 ggcggacggc acggccgagg tcgacgccga ccagatggtc cgcgaggtcg gcgaggtcat
     4801 cgacggcgca ctgaagggca tcgaccccgg cctggtcaag gccgtcgtga tggacacgtt
     4861 cgcctcatcg ctggtgtgcg tggatgccga gggagacgcg ctcaccccct gcatcaccta
     4921 cgccgactcg cggtcatcgg agcatctcgc gaagctgcga tcgcagctcg acgaggatga
     4981 ggtgcaccag cgcaccggca cccggctgca caccagctat cacccgccgc ggctgctttg
     5041 gctgcaggac gagttcccgg aggtgttcgc caagacggcg aagttcctct cgctcggcga
     5101 gtacgtgtac gcgaagctcg ccggcatcga gggcgcggcg acctcgacga tggcgtgggc
     5161 gggcatcctg aaccgccaca cctgcgagct cgatgaggag ctgctggccg ccgtgggcgt
     5221 cgaccgcagc cggttcgcgc ccgtggtcga cccgtccgag ccgatcaccg acgtctccgc
     5281 cgaggctctg aggcgctggc cggcgctcga gggcgcggcg tggttccccg ccgtccccga
     5341 cggctatgcc tcgaacatcg gcgtcggggc gagcaccccg tccgtcgcgg ccatgtcggc
     5401 agccacctcg ggggcgatcc gagtgatcgt cgacggcacg ccggacgtgc tcccgacggg
     5461 tctgtgggcg taccgcgtgt cgaagacgca gtccatcgtc ggcggtgcgc tcaacgatgt
     5521 cggccgtgtc acgctgtggc tgcagtcgac gctggccccg ctgacctacg agcagatcga
     5581 tgaggtcttc cgcgcggctc cctcggaggg cacgcccctc gtgctgccct tcctcacagg
     5641 cgagcgtgcg acgggctggg cgggtgacgc gcgtgccgtg ctcaccggcg tctcctcggc
     5701 ctccggtccg cgcgagctgt ggcgcggagc cgtcgagggg atcgcgatct cctatgcgcg
     5761 cgtcttcgag cagctgctcg aggtcgcgcc cggcatccgc agcgtgatcg cgtcgggcgg
     5821 cgtcaccagc gcctaccccg gcttcatgca tccggtggcc caggcgctgg gattcccggt
     5881 ggaagtggtc caggtcaagc gcgtgaccat gcgcggcgcg gccgcgctgg cgctgtcggt
     5941 cctccaggcg gagcatccgc tcgccaccat cccgcagacg agcctgaccg agccggatgc
     6001 cgcgcagggg gactactacg acgacctgcg ccgccggttc gagaatgtct accagggcgt
     6061 catcgcgaag tgatcgccgg ctgaccgccg ggtgacacga agagggcggg catccgat
//
LOCUS       DATMSX010000594         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_151454,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000594 DATMSX010000000
VERSION     DATMSX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151454"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VN041_19130"
     CDS             complement(<1..272)
                     /locus_tag="VN041_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HWU31201.1"
                     /translation="MNRARMYAVLGFLLAGGVGIISSTQTWITVHRSDAAAPLLVSGA
                     EAMPLLAPLSLAVLALGGALSIAGRALRYVFAVIGLGAVVALVTGTL"
     gene            complement(274..624)
                     /gene="hisI"
                     /locus_tag="VN041_19135"
     CDS             complement(274..624)
                     /gene="hisI"
                     /locus_tag="VN041_19135"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWU31202.1"
                     /translation="MTTLDADVSKVAWNADGLAPVVVQQFDTLEVLMLAWMDAEALRR
                     TLTGGRAVYWSRSRQEYWRKGDTSGNTQKVHAVAVDCDGDTILLTVDQHGPACHTGTR
                     TCFDGRGLTVAESE"
     gene            complement(621..1382)
                     /gene="hisF"
                     /locus_tag="VN041_19140"
     CDS             complement(621..1382)
                     /gene="hisF"
                     /locus_tag="VN041_19140"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWU31203.1"
                     /translation="MTLAARVIPCLDVAAGRVVKGVNFKDLRDMGDPVELAAHYAAQG
                     ADEITFLDVTATVDDRSTTFDVVQRTAEQVFVPLTVGGGVRSVADVARLLSVGADKVG
                     VNSAAIARPELIDEIADRFGAQVLVLSLDIKRATTTESGFVVTTHGGRAETTLDALDW
                     AREAIERGAGELLVNSIDADGTKDGFDLELVRLMREVSSVPVIASGGAGTAADFPPAI
                     EAGADAVLAASVFHSGQLTVGDVKDALRADGVVVR"
     gene            complement(1382..2224)
                     /gene="hisG"
                     /locus_tag="VN041_19145"
     CDS             complement(1382..2224)
                     /gene="hisG"
                     /locus_tag="VN041_19145"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWU31204.1"
                     /translation="MLRIAVPNKGSLSETAAEMLAEAGYAGRRDPKTLHVVDADSDVE
                     FFYLRPRDIATYVASGALDVGITGRDLLRDVRQDDAREIEQLGFARSTFRFAAPPGKY
                     STIQDLEGVRVASAYPGLVDDFLREHGVNAVLVPLDGAVESAVRLGVADVIADVVETG
                     TTLRQAGLEIFGPVIIESEAVLISRPGDVPGTARLLRRLRGVMVARRFVLLDYDLPAH
                     LVDQATAIAGGRESPTISPLRDPEWVAVRVMIPRKGMNQVMDDLYALGARAILVTAIH
                     AARL"
     gene            complement(2263..2526)
                     /locus_tag="VN041_19150"
     CDS             complement(2263..2526)
                     /locus_tag="VN041_19150"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWU31205.1"
                     /translation="MKTFDELFAELQRTAETRPEGSGTVAQLDRGVHAIGKKIVEEAA
                     EVWMAAEYESDENAAEEISQLLYHLQVMMLAKGLSLEDVYRHL"
     gene            complement(2523..3203)
                     /gene="rpe"
                     /locus_tag="VN041_19155"
     CDS             complement(2523..3203)
                     /gene="rpe"
                     /locus_tag="VN041_19155"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWU31206.1"
                     /translation="MSLPEAPRINPSILAADFVNMQADLARIATADFAHVDVMDNHFV
                     PNLTFGPQMVERIQATSPIPLDVHLMITDPDRWAPGYAELGAASVTFHLEAAADPVAL
                     ARRLRSIGSRAGVAIKPGTAGESLYEILDEFDQILVMTVEPGFGGQGFMAETMPKLRA
                     LRQEARRRGSDVWLQVDGGISESTIAQAAEAGADTFVAGSAVYGAEDTAAAVTRLREL
                     ARAASLES"
     gene            complement(3207..>3542)
                     /locus_tag="VN041_19160"
     CDS             complement(3207..>3542)
                     /locus_tag="VN041_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA small subunit methyltransferase B"
                     /protein_id="HWU31207.1"
                     /translation="RKSPADVAELVELQTQLLDAAIEALAPGGIVAYVTCSPHLAETT
                     GVVSEVLRRRDDVAPLDAQAVMTEVAEAPIDLAPNPDGITRHVQLWPHRHGTDAMFLA
                     LLQRTAQEG"
BASE COUNT          523 a         1227 c         1264 g          528 t
ORIGIN      
        1 agcgtgccgg tgaccagcgc gaccacggcc ccgaggccga tcaccgcgaa gacgtagcgc
       61 agcgcgcgtc cggcgatgga cagagctcct ccgagggcca gcactgccag tgacagcggc
      121 gcgagcagcg gcatggcctc ggcgccggag acgagcagcg gtgcggcggc gtcggagcgg
      181 tgcacggtga tccaggtctg cgtcgaggag atgatgccga cgccaccggc gagcaggaac
      241 cccagcaccg cgtacatgcg cgcgcgattc atctcactcg ctctctgcga cggtcagtcc
      301 gcgaccgtcg aagcaggtgc gcgtaccggt gtggcaggcg ggtccgtgct ggtcgaccgt
      361 gagcaggatc gtgtcgccgt cgcagtcgac ggccacggcg tgcaccttct gcgtgttgcc
      421 cgaggtgtcg cccttgcgcc agtactcctg ccgggaccgc gaccagtaca cggcacgacc
      481 gcctgtgagc gtgcgccgca gcgcctcggc atccatccat gccagcatca ggacctcgag
      541 ggtgtcgaac tgctgcacca ccacgggggc gagcccgtcg gcgttccagg cgaccttcga
      601 gacgtccgcg tcgagcgtcg tcatcgcacc accaccccgt ccgcgcggag cgcgtccttc
      661 acgtcgccga ccgtgagctg cccgctgtgg aacacgctcg cggcgagaac ggcatccgct
      721 cccgcctcga tcgccggcgg gaagtccgcg gccgtgcctg cgccgccgga ggcgatcacc
      781 ggcaccgagg acacctcgcg catgaggcgg acgagctcca ggtcgaaacc gtctttcgtg
      841 ccgtcggcgt cgatggagtt caccagcagc tcgccggcac cccgctcgat ggcctcacgc
      901 gcccagtcca gggcatccag ggtggtctcg gcgcgcccac cgtgcgtcgt caccacgaag
      961 cccgattccg tggtcgtggc gcgcttgatg tcgagcgaga gcaccagcac ctgtgcaccg
     1021 aagcggtcgg cgatctcgtc gatcagctcc ggacgcgcga tggcggccga gttcacgccg
     1081 accttgtccg caccgaccga gagcagtcgc gcgacgtccg cgacgctgcg cactcccccg
     1141 cccaccgtga gcggcacgaa gacctgctcg gctgtgcgct gcacgacgtc gaacgtcgtc
     1201 gagcggtcat cgaccgtggc ggtgacgtcg aggaaggtga tctcgtcggc gccctgcgcg
     1261 gcgtaatgcg cggccagctc caccggatcg cccatgtcgc gcagatcctt gaagttcacg
     1321 cccttgacca cgcggccggc ggccacgtcg aggcacggga tcacgcgtgc ggccagggtc
     1381 atcagagcct cgcggcgtgg atcgcggtga cgaggatggc ccgggcgccg agcgcgtaca
     1441 ggtcgtccat cacctggttc atgcccttgc gcgggatcat gacgcgcacg gcgacccact
     1501 ccgggtcgcg cagcggggag atcgtcggcg actcacggcc gccggcgatg gccgtggcct
     1561 ggtcgaccag gtgcgcaggc aggtcgtagt ccagcagcac gaagcgccgg gcgaccatga
     1621 cgccgcggag gcggcgcagc aggcgcgcgg tgccggggac gtcccccggc cggctgatca
     1681 gcacggcctc ggactcgatg atcacggggc cgaagatctc caggcccgcc tggcgcaggg
     1741 tggtgcccgt ctcgacgaca tccgcgatca catcggcgac gccgagcctc acggccgact
     1801 cgacggcgcc gtccagcggg acgagcaccg cgttcacgcc gtgctcgcgc aggaagtcgt
     1861 ccaccaggcc cgggtaggcg gacgccacgc ggacgccctc caggtcctgg atggtcgagt
     1921 acttcccggg aggcgcagcg aaccggaagg tcgagcgggc gaagcccagc tgctcgatct
     1981 cacgcgcgtc gtcctgtcgc acgtcgcgca gcaggtcgcg tccggtgatc cccacgtcga
     2041 gggcgccgga tgccacgtag gtggcgatgt cacgggggcg gaggtagaag aattcgacgt
     2101 cgctgtcggc gtcgacgacg tgcagggtct tgggatcgcg acggccggcg tacccggcct
     2161 ccgcgagcat ctcggcggct gtctcggaga gcgagccctt gttcggaacg gcgatgcgca
     2221 gcatggatgt ccttctgttc gagcggtctg aggggagcac gctcacagat gtcggtagac
     2281 gtcctccagg ctcagcccct tggcgagcat catcacctgc aggtggtaga gcagctggga
     2341 gatctcctca gctgcgttct cgtcgctctc gtactccgcg gccatccata cctcggcggc
     2401 ctcttcgacg atcttcttgc cgatcgcatg cacgccgcgg tccagctgcg cgaccgtgcc
     2461 cgatccttcc ggacgggtct cggcggtgcg ctggagctcg gcgaacagct cgtcgaatgt
     2521 cttcacgatt ccaggctagc cgctcgcgcg agctcccgga gccgggtgac ggccgcagcg
     2581 gtgtcctcgg cgccgtacac ggcggacccg gcgacgaacg tgtcggcccc ggcctcggcg
     2641 gcctgggcga tcgtggactc ggagatgccg ccgtcgacct gcagccacac gtccgagccg
     2701 cgacgccgcg cctcctggcg cagcgcgcgc agcttgggca tggtctcggc catgaagccc
     2761 tggccgccga agcccggctc gacggtcatc accaggatct ggtcgaactc gtcgaggatc
     2821 tcgtacaggg actcccctgc cgtgccgggt ttgatggcga cgccggcgcg tgagccgatc
     2881 gaccggagcc gtcgcgcgag cgccaccgga tcggccgcgg cctcgaggtg gaacgtgacg
     2941 ctcgcggccc ccagttccgc gtagccgggc gcccagcgat cgggatcggt gatcatcagg
     3001 tgcacgtcga gggggatcgg actggtcgcc tggatgcgct ccaccatctg cggcccgaaa
     3061 gtgaggttcg gcacgaagtg gttgtccatc acgtcgacat gggcgaagtc ggcggtcgcg
     3121 atgcgggcga ggtccgcctg catgttgacg aagtcggcgg cgaggatgct ggggttgatg
     3181 cggggtgctt ccgggaggct catggctcat ccttcctggg cggtgcgctg cagcagcgcg
     3241 aggaacatgg cgtcggtgcc gtgtctgtgc ggccacagct gcacgtgacg ggtgatgccg
     3301 tcagggttcg gggcgaggtc gatcggcgcc tcggccacct cggtcatgac ggcctgcgcg
     3361 tcgagcggcg cgacgtcatc gcgccggcgg aggacctcgc tcacgacgcc ggtggtctcc
     3421 gccaggtgtg gcgagcaggt cacgtaggcg acgatcccgc ccggagccag cgcctcgatg
     3481 gcggcgtcga gcagctgcgt ctgcagctcg accaactcgg cgacatcggc cggcgacttg
     3541 cg
//
LOCUS       DATMSX010000595         4632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_151542,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000595 DATMSX010000000
VERSION     DATMSX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151542"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VN041_19165"
     CDS             complement(<1..225)
                     /locus_tag="VN041_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWU31208.1"
                     /translation="MTVAVHVTQPVRATVDEIVPRLIADLVASRVTAGDPTLWGPDAE
                     AEASTRLGWVDAVSVSRPLVAEIVALRDDLA"
     gene            complement(222..1334)
                     /gene="tal"
                     /locus_tag="VN041_19170"
     CDS             complement(222..1334)
                     /gene="tal"
                     /locus_tag="VN041_19170"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWU31209.1"
                     /translation="MSTPTEDLSAAGVSIWLDDLSRTRIQSGNLQQLIETRSVVGVTT
                     NPTIFANSMTNPDDTSYDAQVHELAQSGASAADAVFAATTQDVRDALDVFRPVYDATN
                     HVDGRVSIEVSPDLAHDTEGTIAQAKELWERVDRPNLLVKIPATKAGLPAIAAAIGAG
                     ISVNVTLIFSLERYAEVIDAYLTGLETAKAAGIDLSTIHSVASFFVSRVDTETDKRLA
                     AIGTDEAAALRSKAGLANARLAYELYEKTFASDRAKALLDAGATLQRPLWASTGVKDP
                     ALPDTLYVTGLVAAGVVNTMPEKTLEATFDHGVVTGDTITDGYAQAHQVFEDLKSVGI
                     DFDDVTQVLEDEGVAKFIDSWHGLLEQVAKGLEAAR"
     gene            complement(1331..3427)
                     /gene="tkt"
                     /locus_tag="VN041_19175"
     CDS             complement(1331..3427)
                     /gene="tkt"
                     /locus_tag="VN041_19175"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWU31210.1"
                     /translation="MAELQWDEIDRRAVDTARVLAADAVQKVGNGHPGTAMSLAPVAY
                     LLYQRVMRHDPADVDWLGRDRFILSVGHSSLTQYIQLYLGGFGLELSDLEALRTWGSL
                     TPGHPEYHHTRGIEITTGPLGQGLASSVGFAYAARYERGLFDPDAAAGTSPFDHFVYV
                     IAGDGDLQEGVTSEASSLAGHQQLGNLIAIYDSNQISIEGDTDVAFTEDVRARYESYG
                     WQVLEVDWRRTGHYVEDVQELDDAISQAKAETEKPSLIILKTIIGWPAPNKSGTESVH
                     GNALGADEVAATKKVLGFDPEKSFVVEDAVIAHTRELGARAAEAHAAWQESFDAWAAA
                     NPERKALLDRLEAHALPENIEDALPVFESGTEVSTRAASGKVLNALAAELPELWGGSA
                     DLAGSNNTTIEGAASFIPAEWSTHDWKGSPYGRVLHFGIREHAMGSILNGITLHGPTR
                     AYGGTFLIFSDYMRPAVRLAALMNIPSTFVWTHDSIALGEDGPTHQPIEQIATLRAIP
                     NFTVVRPADGNETSAAWLEILRRHGGPVGIALTRQNVPNFARGEGAASGDVFASASNT
                     AKGAYVLAEAPNGTPDVILIGTGSEVQLAVAARETLAAEGINARVVSAPSLEWFAEQD
                     EAYRESVLPKAVTARVSVEAGSVLSWRGIVGDRGRSVGIDHFGASADYKTLFQKFGIT
                     ADAVVEAARESIKENA"
     gene            3660..4589
                     /locus_tag="VN041_19180"
     CDS             3660..4589
                     /locus_tag="VN041_19180"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWU31211.1"
                     /translation="MAISSTSQTAETTYSVGQKIRGYFVLTKPRVMELLLVSTVPVMI
                     LALGGLPDLRELWLIIATVIGGAMSAGSAASFNMYLDRDIDVHMKRTENRPLVTGEVS
                     PRGALVFSWALAVASTVWLLAFTNWLAASLSVFAIFFYVVVYTMILKRRTEQNIIWGG
                     IAGCFPVLIGWTAVTGSLDWPPFVLFVLIFLWTPPHYWPLSMKYKDDYDDVDVPMLGV
                     TRPAYQVGLQVILYAWATVACSLLLVPVAQMGIVYTASALVFGGWFIYESHRLYNRAL
                     RGTEPRPMRVFHASITYLTLIFLAVAIDPLLPF"
BASE COUNT          662 a         1545 c         1637 g          788 t
ORIGIN      
        1 cgcgagatcg tcgcggagcg cgacgatctc ggcgacgagc gggcgcgaca cggagacggc
       61 gtcgacccag ccgagccggg tggacgcctc ggcctcggca tccggacccc acagcgtggg
      121 atcgcctgcg gtgacacgtg acgcgacgag gtcggcgatg agccggggga cgatctcgtc
      181 caccgtggcc ctgacgggct gcgtcacgtg caccgcgacg gtcatcgtgc ggcttccagg
      241 cccttggcga cctgctccag cagaccgtgc caggagtcga tgaacttggc gacgccctcg
      301 tcctcgagca cctgggtcac gtcgtcgaag tcgatgccga cggacttgag gtcctcgaac
      361 acctggtgcg cctgggcgta gccgtcggtg atggtgtcgc cggtgaccac accgtggtcg
      421 aaggtggcct cgagggtctt ctccggcatg gtgttcacga cgcccgccgc gaccaggccg
      481 gtcacgtaca gggtgtcggg cagtgccggg tccttgacac cggtcgacgc ccacagcgga
      541 cgctgcaggg tggcgcctgc gtcgagcagc gccttggcgc ggtcgctcgc gaaggtcttc
      601 tcgtacagct cgtaggccag ccgcgcgttg gcgaggcccg ccttgctgcg cagcgcggcc
      661 gcctcgtcgg tgccgatggc ggccagccgc ttgtcggtct ccgtgtcgac gcgcgagacg
      721 aagaaggacg cgaccgagtg gatggtcgag aggtcgatgc cggcggcctt cgccgtctcg
      781 agaccggtca ggtacgcgtc gatgacctcg gcgtaccgct ccaggctgaa gatcagggtg
      841 acgttgacgc tgatgccggc tccgatcgcc gcagcgatgg cgggcaggcc ggccttggtc
      901 gcggggatct tcacgagcag gttgggacgg tcgacgcgct cccacagctc cttggcctgg
      961 gcgatcgtgc cctcggtgtc gtgcgcgaga tcgggggaga cctcgatgga gacgcggccg
     1021 tcgacgtggt tcgtggcgtc gtagacggga cggaacacgt ccagggcgtc gcggacgtcc
     1081 tgcgtggtcg ccgcgaagac ggcgtcggcg gcggatgcac cggactgggc cagctcatgc
     1141 acctgcgcgt cgtacgaggt gtcgtccggg ttggtcatcg agttggcgaa gatcgtcggg
     1201 ttggtggtga cgccgacgac gctgcgggtc tcgatcagct gctggaggtt gcccgactgg
     1261 atgcgggtgc gcgacaggtc gtcgagccag atgctgacgc cggcggcgga gagatcttcg
     1321 gtgggggtgc tcatgcgttc tccttgatgg actcgcgtgc cgcctcgacg acggcgtcgg
     1381 cggtgatgcc gaacttctgg aacagggtct tgtagtcggc ggaggcgccg aagtggtcga
     1441 tgccgacgga gcggccgcgg tcgccgacga tgccgcgcca gctgagcacg gagcccgcct
     1501 cgacggagac gcgggccgtg acggccttcg gcagcaccga ctcgcggtac gcctcgtcct
     1561 gctcggcgaa ccactccagc gagggagcgg agacgacgcg ggcgttgatg ccctcggcgg
     1621 cgagcgtctc gcgggcggcg acagccagct gcacctccga gcccgtgccg atcaggatca
     1681 cgtcgggggt gccgttcgga gcctcagcga gcacataggc gcccttggcg gtgttcgacg
     1741 ccgaggcgaa cacatcgccg gatgccgcgc cctcgccgcg cgcgaagttc ggcacgttct
     1801 ggcgggtcaa cgcgatgccg accgggccgc cgtgacggcg caggatctcc agccatgcgg
     1861 ccgaggtctc gttgccgtcg gccggacgca ccaccgtgaa gttcgggatg gcgcgcagcg
     1921 tggcgatctg ctcgatcggc tggtgcgtgg ggccgtcctc gccgagcgcg atcgagtcgt
     1981 gcgtccatac gaacgtgctc gggatgttca tcagcgcggc gagacgaacg gcgggccgca
     2041 tgtagtcgct gaagatcagg aacgtgccgc cgtaggcgcg cgtcggtccg tgcagcgtga
     2101 tgccgttcag gatcgagccc atcgcgtgct cgcggatgcc gaagtgcagc acgcggccgt
     2161 aagggctgcc cttccagtcg tgggtcgacc actcggcggg gatgaacgac gcggcgccct
     2221 cgatcgtggt gttgttggag ccggcgaggt cggcggagcc gccccacagc tcgggaagct
     2281 cagcggccag ggcgttcagc accttgccgg atgcggcgcg cgtcgacacc tcggtgccgc
     2341 tctcgaacac cggaagcgcg tcctcgatgt tctcggggag ggcgtgcgcc tcgagacggt
     2401 ccagcagcgc cttgcgctcg gggttcgccg cagcccaggc gtcgaacgac tcctgccagg
     2461 cggcatgcgc ctccgcggcg cgtgcgccca gctcgcgggt gtgcgcgatc accgcatcct
     2521 cgacgacgaa ggacttctcg gggtcgaagc ccagcacctt cttggtggcg gcgacctcgt
     2581 cggcgcccag cgcgttgccg tgcacggact cggtgccgga cttgttgggt gccggccagc
     2641 cgatgatggt cttcaggatg atcagcgacg gcttctcggt ctccgccttg gcctgggaga
     2701 tcgcgtcgtc cagctcctgc acgtcctcga cgtagtgccc ggtgcgacgc cagtcgacct
     2761 cgagcacctg ccagccgtag gactcgtagc gcgcgcggac gtcctcggtg aacgccacgt
     2821 cggtgtcgcc ctcgatggag atctggttgg agtcgtagat cgcgatcagg ttgccgagct
     2881 gctggtggcc ggcgagcgac gacgcctcgc tggtcacgcc ctcctgcagg tcgccgtcac
     2941 cggcgatcac gtagacgaag tggtcgaagg ggctcgtgcc cgccgcggca tccggatcga
     3001 acagaccgcg ctcatagcgg gcggcgtacg cgaagccgac cgacgacgcc aggccctgac
     3061 ccaggggacc ggtggtgatc tcgatgcccc tggtgtggtg gtactcgggg tgacccggcg
     3121 tgagcgatcc ccaggtgcgc agcgcctcga ggtcgctcag ctccaggccg aacccaccga
     3181 ggtacagctg gatgtactgg gtgagcgacg agtggcccac cgacaggatg aagcggtcac
     3241 ggccgagcca gtccacgtcg gccgggtcgt ggcgcatcac gcgctggtag agcaggtacg
     3301 ccaccggggc gaggctcatc gccgtcccgg ggtggccgtt gccgaccttc tggacggcat
     3361 ccgccgccag tactcgggcg gtgtccaccg ctcgacggtc gatctcgtcc cactgaagct
     3421 ctgccacggt catactcctt ctgacacatt cggaggcgcc cgtattcctg cgcgctccgc
     3481 gtcgtcacgg agaagtggtt gcacagcgca aggggcacgc gagtggtttc agcatacccg
     3541 cgcgatttca cccgccccag gtgcatttca ccgcgctgca ccgaccgctc cgggcgtctc
     3601 tccggcactc ggcacgcact agactggaag gagtgcccgc atgcacgcag aaggagggga
     3661 tcgcgatctc gtcgacatcg cagaccgctg agaccacgta ctcggtcggt cagaagatcc
     3721 gcggttactt cgttctcacg aagccccgtg tcatggagct gctgctcgtc tccaccgttc
     3781 ccgtgatgat cctcgccctc ggcggtcttc ccgacctgcg cgagctctgg ctgatcatcg
     3841 ccaccgtcat cggcggcgcc atgagcgcgg gttccgcggc gtcgttcaac atgtacctcg
     3901 accgcgacat cgacgtgcac atgaagcgca ccgagaaccg gcccctggtc accggcgagg
     3961 tgtcgccgcg cggagccctg gtgttctcgt gggccctcgc agtggcctcc acggtctggc
     4021 tgctcgcctt caccaactgg ctggcggcgt cgctgtcggt cttcgcgatc ttcttctacg
     4081 tcgtggtcta cacgatgatc ctcaagcgcc gcaccgagca gaacatcatc tggggcggca
     4141 tcgcgggatg cttccccgtg ctgatcggct ggaccgcggt gacgggttcg ctggattggc
     4201 cgccgttcgt gctgttcgtg ctgatcttcc tctggacgcc gccgcactac tggccgctgt
     4261 cgatgaagta caaggacgac tacgacgacg tcgacgtgcc catgctcggc gtgacgcgtc
     4321 cggcctacca ggtcgggctg caggtcatcc tctacgcgtg ggcgacggtc gcgtgctcgc
     4381 tgctgctcgt ccccgtcgcg cagatgggca tcgtgtacac ggcatccgcc ctcgtcttcg
     4441 gcggctggtt catctacgag tcgcaccgcc tgtacaaccg cgcgctgcgt ggcaccgagc
     4501 cgcgtccgat gcgcgtgttc cacgcgtcca tcacctacct gacgctgatc ttcctggccg
     4561 tcgcgatcga cccgctgctg ccgttctgat cgccgagcac acgaagaggc ccggctcgtc
     4621 catggacgag cc
//
LOCUS       DATMSX010000596         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_170362,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000596 DATMSX010000000
VERSION     DATMSX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170362"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VN041_19185"
     CDS             complement(<1..1392)
                     /locus_tag="VN041_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWU31212.1"
                     /translation="MGTIVRGLAHTLVLTGAARAEEVSAALAEVGDGDQLAPHLVRSG
                     AVTDAQVAEAVALATNHRYVDLSTYDLDPDIIALVPGNLCRRYRLLPLRLMRDELTVA
                     MLDPTDIIALDDVASLTDLRVTAVVAAADALSQAFERFLRSDGELIDLSEQIGESSSA
                     SDVVFTEHLDDQDADAPVVRFVNLLITQAINDRASDIHIEPGEHRLTVRFRIDGVLHE
                     MQSADRSIQDGVISRLKIMSSIDIAERRVPQDGRITVLHEGRSVDLRVATLPTVWGEK
                     IVMRILDNTGQVMTMEDLRFAPTNRERFHDAITRPHGMVLVTGPTGSGKSTTLYTALR
                     TVANPRVNVITVEDPVEYRIPGINQVQVNPRAGLTFATALRSILRSDPDVVLVGEIRD
                     RETAAISVEAALTGHLVLSTLHTNDAPSALTRLTEIGTEPFLVATALSAVVAQRLARR
                     LCTKCRRPFDGDAE"
     gene            1514..>2111
                     /locus_tag="VN041_19190"
     CDS             1514..>2111
                     /locus_tag="VN041_19190"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWU31213.1"
                     /translation="MTPEAPADQPAADDPALIRLARRIPQPVIAVLAVVAVVLGVVLV
                     VRPTTALGVLALLIGAGLVLGGLLELLFPAVRGGRVRMLLAIALMITGVVVLVLPGLT
                     VRAVAIIVGVTLIVRGAIGVSAIVTGREQPADRRVAEVLLGLAGIAFGILAIVWPDIT
                     LLVVAAVFGATLAIAGIAALWRMLRSRGRHPRAPRRDTM"
BASE COUNT          282 a          744 c          741 g          344 t
ORIGIN      
        1 ctcggcgtcg ccgtcgaacg gccggcgaca cttcgtgcac agccggcggg ccaggcgctg
       61 cgcgaccacc gcgctcaatg cggtcgccac caggaacggc tcggtgccga tctcggtgag
      121 ccgggtcagg gcgctcggag cgtcgttcgt gtgcagcgtg gagagcacca ggtggccggt
      181 cagtgcggcc tccaccgaga tcgccgcggt ctcgcggtcg cggatctcgc cgaccagcac
      241 cacatccggg tcggagcgca ggatcgaccg cagcgcggtc gcgaaggtca gccccgcacg
      301 cgggttcacc tgcacctggt tgatgccggg gatgcggtac tccaccgggt cctcgaccgt
      361 gatcacgttc acgcgcgggt tcgccaccgt gcgcagcgcg gtgtacagcg tggtggactt
      421 gccggatccg gtcgggcccg tcaccagcac catgccgtgc ggacgggtga tcgcgtcgtg
      481 gaatcgctcg cggttggtgg gcgcgaagcg cagatcctcc atcgtcatca cctggccggt
      541 gttgtcgagg attcgcatca cgatcttctc gccccacacc gtcggcagcg tcgccacgcg
      601 caggtcgacc gagcgcccct cgtgcagcac cgtgatgcgg ccgtcctgcg gcacgcgccg
      661 ctcggcgatg tcgatcgacg acatgatctt cagacgcgag atcactccgt cctggatgct
      721 gcggtcggcg ctctgcatct catgcagcac gccgtcgatg cggaacctca ccgtcagccg
      781 gtgctcgccc ggctcgatgt ggatgtcact cgcccggtcg ttgatggcct gggtgatcag
      841 caggttcacg aagcggacga cgggtgcatc ggcatcctgg tcgtcgagat gctcggtgaa
      901 gacgacgtcg gatgccgagg acgattcgcc gatctgctcc gacaggtcga tcagttcgcc
      961 gtccgaccgc aggaagcgct cgaaggcctg cgacagggcg tccgccgccg cgacgacggc
     1021 cgtcacccgc aggtcggtca ggctcgcgac gtcgtcgagc gcgatgatgt cggtcgggtc
     1081 gagcatcgcg accgtcagct cgtcgcgcat cagccgcagc gggagcaggc ggtacctgcg
     1141 gcacaggttg cccggcacca gggcgatgat gtccggatcg aggtcgtagg tcgacaggtc
     1201 gacgtagcga tggttggtcg ccagcgcgac cgcctcggcg acctgggcat ccgtcaccgc
     1261 gcccgagcgc accaggtgcg gcgcgagctg atcgccgtca ccgacctccg cgagcgcggc
     1321 ggagacttct tccgcccggg ctgccccggt cagcacgaga gtatgcgcga gtcctcgcac
     1381 gatggtcccc attcccgcag gcgtccccag cctgcagacg cccgcatccc cagcagagcg
     1441 cacgtcgtgg catcatctta cggcgacggg gcggttccga acaggggcga tcgcgcctgg
     1501 ctaggctgac cgcatgacgc ccgaagcgcc cgccgatcag cccgccgccg acgaccccgc
     1561 gctcatccgg ctggcccgcc ggatcccgca gccggtcatc gccgtcctcg ccgtggtggc
     1621 ggtcgtgctc ggcgtcgtgc tcgtcgtgcg cccgaccacc gcgctgggcg tgctcgcact
     1681 tctgatcggc gccggtctcg tgctcggcgg gctgctggag ctactgttcc cggctgtccg
     1741 cggcggacgt gttcggatgc tgctggcgat cgccctcatg atcaccggag tggtcgtgct
     1801 cgtgctcccc ggcctcacgg tgcgggccgt ggcgatcatc gtcggcgtca cgctgatcgt
     1861 gcgcggagcg atcggagtca gcgcgatcgt caccggtcgg gagcagcccg ccgaccgcag
     1921 ggtcgcagag gtgctgctgg gcctcgcggg catcgccttc ggcatcctgg ccatcgtgtg
     1981 gccggacatc accctgctcg tcgtcgcggc cgtgttcggt gcgaccctcg cgatcgccgg
     2041 gatcgcggcg ctgtggcgga tgctgcgttc gcgcggccgg catccgagag ccccgcgtcg
     2101 tgacaccatg g
//
LOCUS       DATMSX010000597         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Microbacterium sp. isolate SMAG_U12997 k141_170571,
            whole genome shotgun sequence.
ACCESSION   DATMSX010000597 DATMSX010000000
VERSION     DATMSX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943598
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Microbacterium sp. (soil metagenome)
  ORGANISM  Microbacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Microbacteriaceae; Microbacterium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:13:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,840
            CDSs (total)                      :: 3,775
            Genes (coding)                    :: 3,756
            CDSs (with protein)               :: 3,756
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 0 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Microbacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170571"
                     /isolate="SMAG_U12997"
                     /isolation_source="soil"
                     /db_xref="taxon:51671"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1287)
                     /gene="aztD"
                     /locus_tag="VN041_19195"
     CDS             complement(85..1287)
                     /gene="aztD"
                     /locus_tag="VN041_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone AztD"
                     /protein_id="HWU31214.1"
                     /translation="MTGSDRKARAVRAAVIASVAGAAILAGCSAQPAAQHDTKPVTET
                     GTSAQGPRIAVAYAGGVLVVDGGSLEVIDDFSSEEFVRVNPAGDDRHVVVTTSRGFQF
                     LDMREPELTDHVVSAAAAGHVVRHAGRTVLYDDATSDTTIFDSAQLAAMEDELPEGQV
                     IPGVTAHHGVSVLLDNGEFATTVGDEKGRTGIRITDADGTEIAVSAQCPGVHGEGAAQ
                     GEALVFGCEDGALLYRDGEITKLDAPDEFGRIGNAYASDDSSHVLMDYKDDTDAEGYL
                     LKNLALLDTQAKTLGTVALPDGIAYTWRGVVRSPSGDGLMIGSDGQLHVIDPATGEIT
                     ASYDAIEPWDSPAEWQQPHPAIAVSGDLVYVTDSANSEIVALDIATGDVVARGELPEV
                     PNEIAVVD"
     gene            complement(1332..>2204)
                     /locus_tag="VN041_19200"
     CDS             complement(1332..>2204)
                     /locus_tag="VN041_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWU31215.1"
                     /translation="VALAVAALSGCSADTGPRPVVYVTTNILGDVASALAGDDLEVVT
                     LMPPGADPHSFEISARQVAGMRDAELVVANGLGLEEGLLHHVDTAEADGVPVFVAGDH
                     VDVIEYDEGPAPDPHFWTDPAQMARVVEELGVELAALAGDGEVDVRARADAYLQSLRE
                     LEDRMATELGAIPREHRALVTNHHVFGYLARRFDVRILGAVIPGGTTLAAPSASDLQE
                     LVDAIDEAGVTTIFAESSQPDRLVQVLADEADRDVVVAELISESLAPPGDPGDTYLDM
                     MRVNTDRLVDGLSP"
BASE COUNT          312 a          774 c          773 g          345 t
ORIGIN      
        1 gcgcaccgcg ccgcggcgac ctgatcgccc cgtcgcggtc gcccacccgg atgccggtga
       61 gggcgaccac gacgggggca cggatcagtc gacgacggcg atctcgttcg gcacctcggg
      121 cagctcacct cgtgcgacca cgtcgcctgt ggcgatgtcg agcgccacga tctcgctgtt
      181 cgctgagtcc gtgacgtaca cgaggtcgcc cgagaccgcg atggcggggt ggggctgctg
      241 ccactcggcg gggctgtccc acggctcgat cgcgtcgtac gacgcggtga tctcgccggt
      301 ggccgggtcg atcacgtgca gctgaccgtc cgagccgatc atgagcccgt cgcccgacgg
      361 cgagcgcacg acgccgcgcc acgtgtaggc gatcccgtcg ggcagtgcga ccgtgccgag
      421 ggtcttcgcc tgagtgtcca gcagggcgag gttcttcagc aggtagcctt cggcatccgt
      481 atcgtccttg tagtccatca gcacgtgcga gctgtcgtcg gaggcgtacg cgttgccgat
      541 ccgcccgaac tcgtcggggg cgtcgagctt ggtgatctcg ccgtcacggt agaggagggc
      601 gccgtcctcg cagccgaaca ccagggcctc gccctgcgcc gcgccctcgc cgtgcactcc
      661 cgggcactgc gcgctgacgg cgatctccgt gccgtcagcg tccgtgatgc ggatgccggt
      721 gcgccccttc tcgtcgccca ccgtcgtcgc gaactcgccg ttgtccagca gcaccgagac
      781 accgtggtgc gccgtcacgc ccggaatcac ctgcccctcg gggagctcat cctccatggc
      841 ggccagctgg gcgctgtcga agatcgtcgt gtcgcttgtt gcgtcgtcgt agagaaccgt
      901 gcgacccgca tgccgcacga cgtgaccggc ggcggccgcg gagacgacat gatcggtcag
      961 ctccggttcc cgcatgtcga ggaactggaa tccccgtgac gtcgtgacca cgacatggcg
     1021 gtcatcgccc gccgggttga cacggacgaa ctcctccgag gagaagtcgt cgatcacctc
     1081 gagacttccg ccgtcgacca cgagcacgcc gcccgcatag gcgacggcga tgcgaggccc
     1141 ctgcgcggag gtgccggtct cggtcacagg cttggtgtcg tgctgcgctg cgggctgcgc
     1201 ggagcatccc gccaggatgg cggcgccggc gacgctcgcg atcacggccg cgcggacggc
     1261 gcgggccttc cggtcggatc ctgtcatggt gttgcttcct ttctgagtac cccgcgaagg
     1321 gggcgttgtg gtcaggggct gaggccgtcg acgaggcggt cggtgttgac gcgcatcatg
     1381 tcgaggtagg tgtcgccggg gtcacccggc ggggcgagcg actcggagat cagctcggcg
     1441 acgacgacat cccgatccgc ctcgtcggcg agcacctgca cgagacggtc cggctgcgag
     1501 gactccgcga agatcgtcgt cacgcccgcc tcgtcgatgg catccacgag ctcctggaga
     1561 tcggaggcgc tgggcgcggc cagcgtcgtg ccccccggga tcactgcgcc caggatgcgc
     1621 acgtcgaatc ggcgggcgag gtagccgaac acatggtggt tcgtgaccaa tgcacggtgc
     1681 tcacgcggga tggcaccgag ctccgtcgcc atccgatcct cgagctcccg caggctctgc
     1741 aggtaggcgt ccgccctggc gcgcacgtcc acttccccat cgccggcgag agccgccagt
     1801 tccacgccga gctcctcgac gacgcgcgcc atctgtgcag ggtccgtcca gaagtgcggg
     1861 tcgggcgctg gaccctcgtc gtactcgatg acgtcgacgt gatctcctgc gacgaagacc
     1921 ggcacaccgt cggcctccgc cgtgtcgacg tgatgcagca ggccctcttc gagccccagg
     1981 ccgttggcga cgacgagctc ggcatcgcgc atccccgcca cctgccgcgc cgagatctcg
     2041 aacgagtgcg gatcggcgcc gggcggcatg agcgtgacga cctcgaggtc gtcgccggcg
     2101 agggccgacg cgacgtcgcc gaggatgttc gtcgtcacgt agacgaccgg acgggggccg
     2161 gtgtccgccg agcagccgct caacgcggcg accgcgaggg cgac
//
